LOCUS       DAKTIO010000001        45591 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.001, whole genome shotgun sequence.
ACCESSION   DAKTIO010000001 DAKTIO010000000
VERSION     DAKTIO010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 45591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45591
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.001"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            102..1220
                     /gene="ald"
                     /locus_tag="O0293_000001"
     CDS             102..1220
                     /gene="ald"
                     /locus_tag="O0293_000001"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HCY0278917.1"
                     /translation="MLVAVVKELKQGEGRVACTPENVRKLTDAGHKVIVEKNAGIGSG
                     FSNDMYEKEGAKIVTHEQAWEADLVIKVKEPHESEYQYFKKNQIIWGFLHLASSKEIV
                     EKMQEVGVTAISGETIIKNGKAELLAPMSAIAGQRSAIMGAYYSEAQHGGQGTLVTGV
                     HENVDIPGSTYVIFGGGVAATNAANVALGLNAKVIIIELNDDRIKYLEDMYAEKDVTV
                     VKSTPENLAEQIKKADVFISTILIPGAKPPKLVTREMVKSMKKGSVLIDIAIDQGGTI
                     ETIRPTTISDPVYEEEGVIHYGVPNQPGAVPRTSTMALAQGNIDYILEICDKGLEQAI
                     KDNEALSTGVNIYQGQVTNQGLASSHDLDYKEILNVIE"
     gene            1315..2355
                     /gene="tdcB"
                     /locus_tag="O0293_000002"
     CDS             1315..2355
                     /gene="tdcB"
                     /locus_tag="O0293_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCY0278918.1"
                     /translation="MTTNTVTLQTAHIVSLGDIEEAKASIKPFIRRTPLIKSMYLSQS
                     ITKGNVFLKLENMQFTGSFKFRGASNKINHLTDEQKEKGIIAASAGNHAQGVALTAKL
                     LGIDATIVMPETAPQAKQQATKGYGAKVILKGKNFNETRLYMEELAKENGMTIVHPYD
                     DKFVMAGQGTIGLEILDDIWNVNTVIVPVGGGGLIAGIATALKSFNPSIHIIGVQSEN
                     VHGMAESFYKRDLTEHRVDSTIADGCDVKVPGEQTYEVVKHLVDEFILVTEEEIEHAM
                     KDLMQRAKIITEGAGALPTAAILSGKINNKWLEDKNVVALVSGGNVDLTRVSGVIEHG
                     LNIADTSKGVVG"
     gene            2386..3708
                     /locus_tag="O0293_000003"
     CDS             2386..3708
                     /locus_tag="O0293_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0278919.1"
                     /translation="MSNGKELQKNIGFFSAFAIVMGTVIGSGVFFKISNVTEVTGTAG
                     MALFVWFLGGIITICAGLTAAELAAAIPETGGLTKYIEYTYGDFWGFLSGWAQSFIYF
                     PANVAALSIVFATQLINLFHLSIGSLIPIAIASALSIVLINFLGSKAGGILQSVTLVI
                     KLIPIIVIVIFGIFQSGDITFSLIPTTGNSGNGFFTAIGSGLLATMFAYDGWIHVGNV
                     AGELKNPKRDLPLAISVGIGCIMAVYLLINATFLLTLPIELLAGNLNAASDTSKILFG
                     ENGGKIITIGILISVYGTINGYTMTGMRVPYAMAERKLLPFSHLFAKLTKSGAPWFGA
                     IIQLIIAIIMMSMGAFDTITNMLIFVIWLFYCMSFVAVIILRKREPNMERPYKVPLYP
                     IIPLIAILAGSFVLINTLFTQFILAIIGILITALGIPVYYYKKKQKAA"
     gene            3865..5256
                     /gene="norB"
                     /locus_tag="O0293_000004"
     CDS             3865..5256
                     /gene="norB"
                     /locus_tag="O0293_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorB"
                     /protein_id="HCY0278920.1"
                     /translation="MEKPSREAFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSF
                     NTDIGTVNIAVSITALFSGMFVVGAGGLADKYGRIKLTNIGIILNILGSLLIIISNIP
                     LLLIIGRLIQGLSAACIMPATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGA
                     VATLLGWRWIFILSIIISLIALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLSLNI
                     LITKGSELGVTSLLFITLLAIAIGSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASN
                     FLLNGVAGTLIVANTFVQRGLGYSSLQAGSLSITYLVMVLIMIRVGEKLLQTLGCKKP
                     MLIGTGVLIVGECLISLTFLPEIFYVICCIIGYLFFGLGLGIYATPSTDTAIANAPLE
                     KVGVAAGIYKMASALGGAFGVALSGAVYAIVSNMTNIYTGAMIALWLNAGMGILSFVI
                     ILLLVPKQNDTQL"
     gene            5654..36919
                     /gene="ebh"
                     /locus_tag="O0293_000005"
     CDS             5654..36919
                     /gene="ebh"
                     /locus_tag="O0293_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyperosmolarity resistance protein Ebh"
                     /protein_id="HCY0278921.1"
                     /translation="MNYRDKIQKFSIRKYTVGTFSTVIATLVFLGFNTSQAHAAETNQ
                     PASVVKQKQQSNNEQTENRESQVQNSQNSQNGQSLSATHENEQPNISQANLVDQKVAQ
                     SSTTNDEQPASQNVNTKKDSATAATTQPDKEQSKHKQNESQSANKNGNDNRAAHVENH
                     EANVVTASDSSDNGNVQHDRNELQAFFDANYHDYRFIDRENADSGTFNYVKGIFDKIN
                     TLLGSNDPINNKDLQLAYKELEQAVALIRTMPQRQQTSRRSNRIQTRSVESRAAEPRS
                     VSDYQNANSSYYVENANDGSGYPVGTYINASSKGAPYNLPTTPWNTLKASDSKEIALM
                     TAKQTGDGYQWVIKFNKGHAPHQNMIFWFALPADQVPVGRTDFVTVNSDGTNVQWSHG
                     AGAGANKPLQQMWEYGVNDPHRSHDFKIRNRSGQVIYDWPTVHIYSLEDLSRASDYFS
                     EAGATPATKAFGRQNFEYINGQKPAESPGVPKVYTFIGQGDASYTISFKTQGPTVNKL
                     YYAAGGRALEYNQLFMYSQLYVESTQDHQQRLNGLRQVVNRTYRIGTTKRVEVSQGNV
                     QTKKVLESTNLNIDDFVDDPLSYVKTPSNKVLGFYSNNANTNAFRPGGAQQLNEYQLS
                     QLFTDQKLQEAARTRNPIRLMIGFDYPDAYGNSETLVPVNLTVLPEIQHNIKFFKNDD
                     TQNIAEKPFSKQAGHPVFYVYAGNQGNASVNLGGSVTSIQPLRINLTSNENFTDKDWQ
                     ITGIPRTLHIENSTNRPNNARERNIELVGNLLPGDYFGTIRFGRKEQLFEIRVKPHTP
                     TITTTAEQLRGTALQKVPVNISGIPLDPSALVYLVAPTNQTTNGGSEADQIPSGYTIL
                     ATGTPDGVHNTITIRPQDYVVFIPPVGKQIRAVVYYNKVVASNMSNAVTILPDDIPPT
                     INNPVGINAKYYRGDEVNFTMGVSDRHSGIKNTTITTLPNGWTSNLTKADKNNGSLSI
                     TGRVSMNQAFNSDITFKVSATDNVNNTTNDSQSKHVSIHVGKISEDAHPIVLGNTEKV
                     VVVNPTAVSNDEKQSIITAFMNKNQNIRGYLASTDPVTVDNNGNVTLHYRDGSSTTLD
                     ATNVMTYEPVVKPEYQTVNAAKTATVTIAKGQSFSIGDIKQYFTLSNGQPIPSGTFTN
                     ITSDRTIPTAQEVSQMNAGTQLYHITATNAYHKDSEDFYISLKIIDVKQPEGDQRVYR
                     TSTYDLTTDEISKVKQAFINANRDVITLAEGDISVTNTPNGANVSTITVNINKGRLTK
                     SFASNLANMNFLRWVNFPQDYTVTWTNAKIANRPTDGGLSWSDDHKSLIYRYDATLGT
                     QITTNDILTMLKATTTVPGLRNNITGNEKSQAEAGGRPNFRTTGYSQSNATTDGQRQF
                     TLNGQVIQVLDIINPSNGYGGQPVTNSNTRANHSNSTVVNVNEPAANGAGAFTIDHVV
                     KSNSTHNASDAVYKAQLYLTPYGPKQYVEHLNQNTGNTTDAINIYFVPSDLVNPTISV
                     GNYTNHQVFSGETFTNTITANDNFGVQSVTVPNTSQITGTVDNNHQHVSATAPNVTSA
                     TNKTINLLATDTSGNTATTSFNVTVKPLRDKYRVGTSSTAANPVRIANISNNATVSQA
                     DQTTIINSLTFTETVPNRSYARASANEITSKTVSNVSRTGNNANVTVTVTYQDGTTST
                     VTVPVKHVIPEIVAHSHYTVQGQDFPAGNGSSASDYFKLSNGSDIADATITWVSGQAP
                     NKDNTRIGEDITVTAHILIDGETTPITKTATYKVVRTVPKHVFETARGVLYPGVSDMY
                     DAKQYVKPVNNSWSTNAQHMNFQFVGTYGPNKDVVGISTRLIRVTYDNRQTEDLTILS
                     KVKPDPPRIDANSVTYKAGLTNQEIKVNNVLNNSSVKLFKADNTPLNVTNITHGSGFS
                     SVVTVSDALPNGGIKAKSSISMNNVTYTTQDEHGQVVTVTRNESVDSNDSATVTVTPQ
                     LQATTEGAVFIKGGDGFDFGHVERFIQNPPHGATVAWHDSPDTWKNTVGNTHKTAVVT
                     LPNGQGTRNVEVPVKVYPVANAKAPSRDVKGQNLTNGTDAMNYITFDPNTNTNGITAA
                     WANRQQPNNQQAGVQHLNVDVTYPGISAAKRVPVTVNVYQFEFPQTTYTTTVGGTLAS
                     GTQASGYAHMQNATGLPTDGFTYKWNRDTTGTNDANWSAMNKPNVAKVVNAKYDVIYN
                     GHTFATSLPAKFVVKDVQPAKPTVTETAAGAITIAPGANQTVNTHAGNVTTYADKLVI
                     KRNGNVVTTFTRRNNTSPWVKEASAATVAGIAGTNNGITVAAGTFNPADTIQVVATQG
                     SGETVSDEQRSDDFTVVAPQPNQATTKIWQNGHIDITPNNPSGHLINPTQAMDIAYTE
                     KVGNGAEHSKTINVVRGQNNQWTIANKPDYVTLDAQTGKVTFNANTIKPNSSITITPK
                     AGTGHSVSSNPSTLTAPAAHTVNTTEIVKDYGSNVTAAEINNAVQVANKRTATIKNGT
                     AMPTNLAGGSTTTIPVTVTYNDGSTEEVQESIFTKADKRELITAKNHLDDPVSTEGKK
                     PGTITQYNNAMHNAQQQINTAKTEAQQVINNERATPQQVSDALTKVRAAQTKIDQAKA
                     LLQNKEDNSQLVTSKNNLQSSVNQVPSTAGMTQQSIDNYNAKKREAETEITAAQRVID
                     NGDATAQQISDEKHRVDNALTALNQAKHDLTADTHALEQAVQQLNRTGTTTGKKPASI
                     TAYNNSIRALQSDLTSAKNSANAIIQKPIRTVQEVQSALTNVNRVNERLTQAINQLVP
                     LADNSALKTAKTKLDEEINKSVTTDGMTQSSIQAYENAKRAGQTESTNAQNVINNGDA
                     TDQQIAAEKTKVEEKYNSLKQAIAGLTPDLAPLQTAKTQLQNDIDQPTSTTGMTSASI
                     AAFNEKLSAARTKIQEIDRVLASHPDVATIRQNVTAANAAKSALDQARNGLTVDKAPL
                     ENAKNQLQHSIDTQTSTTGMTQDSINAYNAKLTAARNKIQQINQVLAGSPTVEQINTN
                     TSTANQAKSDLDHARQALTPDKAPLQTAKTQLEQSINQPTDTTGMTTASLNAYNQKLQ
                     AARQKLTEINQVLNGNPTVQNINDKVTEANQAKDQLNTARQGLTLDRQPALTTLHGAS
                     NLNQAQQNNFTQQINAAQNHAALETIKSNITALNTAMTKLKDSVADNNTIKSDQNYTD
                     ATPANKQAYDNAVNAAKGVIGETTNPTMDVNTVNQKAASVKSTKDALDGQQNLQRAKT
                     EATNAITHASDLNQAQKNALTQQVNSAQNVQAVNDIKQTTQSLNTAMTGLKRGVANHN
                     QVVQSDNYVNADTNKKNDYNNAYNHANDIINGNAQHPVITPSDVNNALSNVTSKEHAL
                     NGEAKLNAAKQEANTALGHLNNLNNAQRQNLQSQINGAHQIDAVNTIKQNATNLNSAM
                     GNLRQAVADKDQVKRTEDYADADTAKQNAYNSAVSSAETIINQTTNPTMSVDDVNRAT
                     SAVTSNKNALNGYEKLAQSKTDAARAIDALPHLNNAQKADVKSKINAASNIAGVNTVK
                     QQGTDLNTAMGNLQGAINDEQTTLNSQNYQDATPSKKTAYTNAVQAAKDILNKSNGQN
                     KTKDQVTEAMNQVNSAKNNLDGTRLLDQAKQTAKQQLNNMTHLTTAQKTNLTNQINSG
                     TTVAGVQTVQSNANTLDQAMNTLRQSIANKDATKASEDYVDANNDKQTAYNNAVAAAE
                     TIINANSNPEMNPSTITQKAEQVNSSKTALNGDENLAAAKQNAKTYLNTLTSITDAQK
                     NNLISQITSATRVSGVDTVKQNAQHLDQAMASLQNGINNESQVKSSEKYRDADTNKQQ
                     EYDNAITAAKAILNKSTGPNTAQNAVEAALQRVNNAKDALNGDAKLIAAQNAAKQHLG
                     TLTHITTAQRNDLTNQISQATNLAGVESVKQNANSLDGAMGNLQTAINDKSGTLASQN
                     FLDADEQKRNAYNQAVSAAETILNKQTGPNTAKTAVEQALNNVNNAKHALNGTQNLNN
                     AKQAAITAINGASDLNQKQKDALKAQANGAQRVSNAQDVQHNATELNTAMGTLKHAIA
                     DKTNTLASSKYVNADSTKQNAYTTKVTNAEHIISGTPTVVTTPSEVTAAANQVNSAKQ
                     ELNGDERLREAKQNANTAIDALTQLNTPQKAKLKEQVGQANRLEDVQTVQTNGQALNN
                     AMKGLRDSIANETTVKTSQNYTDASPNNQSTYNSAVSNAKGIINQTNNPTMDTSAITQ
                     ATTQVNNAKNGLNGAENLRNAQNTAKQNLNTLSHLTNNQKSAISSQIDRAGHVSEVTA
                     TKNAATELNTQMGNLEQAIHDQNTVKQSVKFTDADKAKRDAYTNAVSRAEAILNKTQG
                     ANTSKQDVEAAIQNVSSAKNALNGDQNVTNAKNAAKNALNNLTSINNAQKRDLTTKID
                     QATTVAGVEAVSNTSTQLNTAMANLQNGINDKTNTLASENYHDADSDKKTAYTQAVTN
                     AENILNKNSGSNLDKTAVENALSQVANAKGALNGNHNLEQAKSNANTTINGLQHLTTA
                     QKDKLKQQVQQAQNVAGVDTVKSSANTLNGAMGTLRNSIQDNTATKNGQNYLDATERN
                     KTNYNNAVDSANGVINATSNPNMDANAINQIATQVTSTKNALDGTHNLTQAKQTATNA
                     IDGATNLNKAQKDALKAQVTSAQRVANVTSIQQTANELNTAMGQLQHGIDDENATKQT
                     QKYRDAEQSKKTAYDQAVAAAKAILNKQTGSNSDKAAVDRALQQVTSTKDALNGDAKL
                     AEAKAAAKQNLGTLNHITNAQRTDLEGQINQATTVDGVNTVKTNANTLDGAMNSLQGS
                     INDKDATLRNQNYLDADESKRNAYTQAVTAAEGILNKQTGGNTSKADVDNALNAVTRA
                     KAALNGADNLRNAKTSATNTIDGLPNLTQLQKDNLKHQVEQAQNVAGVNGVKDKGNTL
                     NTAMGALRTSIQNDNTTKTSQNYLDASDSNKNNYNTAVNNANGVINATNNPNMDANAI
                     NGMANQVNTTKAALNGAQNLAQAKTNATNTINNAHDLNQKQKDALKTQVNNAQRVSDA
                     NNVQHTATELNSAMTALKAAIADKERTKASGNYVNADQEKRQAYDSKVTNAENIISGT
                     PNATLTVNDVNSAASQVNAAKTALNGDNNLRVAKEHANNTIDGLAQLNNAQKAKLKEQ
                     VQSATTLDGVQTVKNSSQTLNTAMKGLRDSIANEATIKAGQNYTDASPNNRNEYDSAV
                     TAAKAIINQTSNPTMEPNTITQVTSQVTTKEQALNGARNLAQAKTTAKNNLNNLTSIN
                     NAQKDALTRSIDGATTVAGVNQETAKATELNNAMHSLQNGINDETQTKQTQKYLDAEP
                     SKKSAYDQAVNAAKAILTKASGQNVDKAAVEQALQNVNSTKTALNGDAKLNEAKAAAK
                     QTLGTLTHINNAQRTALDNEITQATNVEGVNTVKAKAQQLDGAMGQLETSIRDKDTTL
                     QSQNYQDADDAKRTAYSQAVNAAATILNKTAGGNTPKADVERAMQAVTQANTALNGIQ
                     NLDRAKQAANTAITNASDLNTKQKEALKAQVTSAGRVSAANGVEHTATELNTAMTALK
                     RAIADKAETKASGNYVNADANKRQAYDEKVTAAENIVSGTPTPTLTPADVTNAATQVT
                     NAKTQLNGNHNLEVAKQNANTAIDGLTSLNGPQKAKLKEQVGQATTLPNVQTVRDNAQ
                     TLNTAMKGLRDSIANEATIKAGQNYTDASQNKQTDYNSAVTAAKAIIGQTTSPSMNAQ
                     EINQAKDQVTAKQQALNGQENLRTAQTNAKQHLNGLSDLTDAQKDAVKRQIEGATHVN
                     EVTQAQNNADALNTAMTNLKNGIQDQNTIKQGVNFTDADEAKRNAYTNAVTQAEQILN
                     KAQGPNTSKDGVETALENVQRAKNELNGNQNVANAKTTAKNALNNLTSINNAQKEALK
                     SQIEGATTVAGVNQVSTTASELNTAMSNLQNGINDEAATKAAQKYTDADREKQTAYND
                     AVTAAKTLLDKTAGSNDNKAAVEQALQRVNTAKTALNGDERLNEAKNTAKQQVATMSH
                     LTDAQKANLTSQIESGTTVAGVQGIQANAGTLDQAMNQLRQSIASKDATKSSEDYQDA
                     NADLQNAYNDAVTNAEGIISATNNPEMNPDTINQKASQVNSAKSALNGDEKLAAAKQT
                     AKSDIGRLTDLNNAQRTAANAEVDQAPNLAAVTAAKNKATSLNTAMGNLKHALAEKDN
                     TKRSVNYTDADQPKQQAYDTAVTQAEAITNANGSNANETQVQAALNQLNQAKNDLNGD
                     NKVAQAKESAKRALASYSNLNNAQSTAAISQIDNATTVAGVTAAQNTANELNTAMGQL
                     QNGINDQNTVKQQVNFTDADQGKKDAYTNAVTNAQGILDKAHGQNMTKAQVEAALNQV
                     TTAKNALNGDANVRQAKSDAKANLGTLTHLNNAQKQDLTSQIEGATTVNGVNGVKTKA
                     QDLDGAMQRLQSAIANKDQTKASENYIDADPTKKTAFDNAITQAESYLNKDHGANKDK
                     QAVEQAIQSVTSTENALNGDANLQRAKTEAIQAIDNLTHLNTPQKTALKQQVNAAQRV
                     SGVTDLKNSATSLNNAMDQLKQAIADHDTIVASGNYTNASPDKQGAYTDAYNAAKNIV
                     NGSPNVITNAADVTAATQRVNNAETGLNGDTNLATAKQQAKDALRQMTHLSDAQKQSI
                     TGQIDSATQVTGVQSVKDNATNLDNAMNQLRNSIANKDDVKASQPYVDADRDKQNAYN
                     TAVTNAENIINATSQPTLDPSAVTQAANQVSTNKTALNGAQNLANKKQETTANINQLS
                     HLNNAQKQDLNTQVTNAPNISTVNQVKTKAEQLDQAMERLINGIQDKDQVKQSVNFTD
                     ADPEKQTAYNNAVTAAENIINQANGTNANQSQVEAALSTVTTTKQALNGDRKVTDAKN
                     NANQTLSTLDNLNNAQKGAVTGNINQAHTVAEVTQAIQTAQELNTAMGNLKNSLNDKD
                     TTLGSQNFADADPEKKNAYNEAVHNAENILNKSTGTNVPKDQVEAAMNQVNATKAALN
                     GTQNLEKAKQHANTAIDGLSHLTNAQKEALKQLVQQSTTVAEAQGNEQKANNVDAAMD
                     KLRQSIADNATTKQNQNYTDASQNKKDAYNNAVTTAQGIIDQTTSPTLDPTVINQAAG
                     QVSTTKNALNGNENLEAAKQQASQSLGSLDNLNNAQKQTVTDQINGAHTVDEANQIKQ
                     NAQNLNTAMGNLKQAIADKDATKATVNFTDADQAKQQAYNTAVTNAENIISKANGGNA
                     TQAEVEQAIKQVNAAKQALNGNANVQHAKDEATALINSSNDLNQAQKDALKQQVQNAT
                     TVAGVNNVKQTAQELNNAMTQLKQGIADKEQTKADGNFVNADPDKQNAYNQAVAKAEA
                     LISATPDVVVTPSEITAALNKVTQAKNDLNGNTNLATAKQNVQHAIDQLPNLNQAQRD
                     EYSKQITQATLVPNVNAIQQAATTLNDAMTQLKQGIANKAQIKGSENYHDADTDKQTA
                     YDNAVTKAEELLKQTTNPTMDPNTIQQALTKVNDTNQALNGNQKLADAKQDAKTTLGT
                     LDHLNDAQKQALTTQVEQAPDIATVNNVKQNAQNLNNAMTNLNNALQDKTETLNSINF
                     TDADQAKKDAYTNAVSHAEGILSKANGSNASQTEVEQAMQRVNEAKQALNGNDNVQRA
                     KDAAKQVITNANDLNQAQKDALKQQVDAAQTVANVNTIKQTAQDLNQAMTQLKQGIAD
                     KDQTKANGNFVNADTDKQNAYNNAVAHAEQIISGTPNANVDPQQVAQALQQVNQAKGD
                     LNGNHNLQVAKDNANTAIDQLPNLNQPQKTALKDQVSHAELVTGVNAIKQNADALNNA
                     MGTLKQQIQANSQVPQSVDFTQADQDKQQAYNNAANQAQQIANGIPTPVLTPDTVTQA
                     VTTMNQAKDALNGDEKLAQAKQEALANLDTLRDLNQPQRDALRNQINQAQALATVEQT
                     KQNAQNVNTAMSNLKQGIANKDTVKASENYHDADADKQTAYTNAVSQAEGIINQTTNP
                     TLNPDEITRALTQVTDAKNGLNGEAKLATEKQNAKDAVSGMTHLNDAQKQALKGQIDQ
                     SPEIATVNQVKQTATSLDQAMDQLSQAINDKAQTLADGNYLNADPDKQNAYKQAVAKA
                     EALLNKQSGTNEVQAQVESITNEVNAAKQALNGNDNLANAKQQAKQQLANLTHLNDAQ
                     KQSFESQITQAPLVTDVTTINQKAQTLDHAMELLRNSVADNQTTLASEDYHDATAQRQ
                     NDYNQAVTAANNIINQTTSPTMNPDDVNGATTQVNNTKVALDGDENLAAAKQQANNRL
                     DQLDHLNNAQKQQLQSQITQSSDIAAVNGHKQTAESLNTAMGNLINAIADHQAVEQRG
                     NFINADTDKQTAYNTAVNEAAAMINKQTGQNANQTEVEQAITKVQTTLQALNGDHNLQ
                     VAKTNATQAIDALTSLNDPQKTALKDQVTAATLVTAVHQIEQNANTLNQAMHGLRQSI
                     QDNAATKANSKYINEDQPEQQNYDQAVQAANNIINEQTATLDNNAINQAATTVNTTKA
                     ALHGDVKLQNDKDHAKQTVSQLAHLNNAQKHMEDTLIDSETTRTAVKQDLTEAQALDQ
                     LMDALQQSIADKDATRASSAYVNAEPNKKQSYDEAVQNAESIIAGLNNPTINKGNVSS
                     ATQAVISSKNALDGVERLAQDKQTAGNSLNHLDQLTPAQQQALENQINNATTRGEVAQ
                     KLTEAQALNQAMEALRNSIQDQQQTEAGSKFINEDKPQKDAYQAAVQNAKDLINQTNN
                     PTLDKAQVEQLTQAVNQAKDNLHGDQKLADDKQHAVTDLNQLNGLNNPQRQALESQIN
                     NAATRGEVAQKLAEAKALDQAMQALRNSIQDQQQTESGSKFINEDKPQKDAYQAAVQN
                     AKDLINQTGNPTLDKSQVEQLTQAVTTAKDNLHGDQKLARDQQQAVTTVNALPNLNHA
                     QQQALTDAINAAPTRTEVAQHVQTATELDHAMETLKNKVDQVNTDKAQPNYTEASTDK
                     KEAVDQALQAAESITDPTNGSNANKDAVDQVLTKLQEKENELNGNERVAEAKTQAKQT
                     IDQLTHLNADQIATAKQNIDQATKLQPIAELVDQATQLNQSMDQLQQAVNEHANVEQT
                     VDYTQADSDKQNAYKQAIADAENVLKQNANKQQVDQALQNILNAKQALNGDERVALAK
                     TNGKHDIDQLNALNNAQQDGFKGRIDQSNDLNQIQQIVDEAKALNRAMDQLSQEITDN
                     EGRTKGSTNYVNADTQVKQVYDETVDKAKQALDKSTGQNLTAKQVIKLNDAVTAAKKA
                     LNGEERLNNRKAEALQRLDQLTHLNNAQRQLAIQQINNAETLNKASRAINRATKLDNA
                     MGAVQQYIDEQHLGVISSTNYINADDNLKANYDNAIANAAHELDKVQGNAIAKAEAEQ
                     LKQNIIDAQNALNGDQNLANAKDKANAFVNSLNGLNQQQQDLAHKAINNADTVSDVTD
                     IVNNQIDLNDAMETLKHLVDNEIPNAEQTVNYQNADDNAKTNFDDAKRLANTLLNSDN
                     TNVNDINGAIQAVNDAIHNLNGDQRLQDAKDKAIQSINQALANKLKEIEASNATDQDK
                     LIAKNKAEELANSIINNINKATSNQAVSQVQTAGNHAIEQVHANEIPKAKIDANKDVD
                     KQVQALIDEIDRNPNLTDKEKQALKDRINQILQQGHNGINNAMTKEEIEQAKAQLAQA
                     LQDIKDLVKAKEDAKQDVDKQVQALIDEIDQNPNLTDKEKQALKYRINQILQQGHNDI
                     NNALTKEEIEQAKAQLAQALQDIKDLVKAKEDAKNAIKALANAKRDQINSNPDLTPEQ
                     KAKALKEIDEAEKRALQNVENAQTIDQLNRGLNLGLDDIRNTHVWEVDEQPAVNEIFE
                     ATPEQILVNGELIVHRDDIITEQDILAHINLIDQLSAEVIDTPSTATISDSLTAKVEV
                     TLLDGSKVIVNVPVKVVEKELSVVKQQAIESIENAAQQKINEINNSVTLTLEQKEAAI
                     AEVNKLKQQAIDHVNNAPDVHSVEEIQQQEQAHIEQFNPEQFTIEQAKSNAIKSIEDA
                     IQHMIDEIKARTDLTDKEKQEAIAKLNQLKEQAIQAIQRAQSIDEISEQLEQFKAQMK
                     AANPTAKELAKRKQEAISRIKDFSNEKINSIRNSEIGTADEKQAAMNQINEIVLETIR
                     DINNAHTLQQVEAALNNGIARISAVQIVTSDRAKQSSSTGNESNSHLTIGYGTANHPF
                     NSSTIGHKKKLDEDDDIDPLHMRHFSNNFGNVIKNAIGVVGISGLLASFWFFIAKRRR
                     KEDEEEELEIRDNNKDSIKETLDDTKHLPLLFAKRRRKEDEEDVTVEEKDSLNNGESL
                     DKVKHTPFFLPKRRRKEDEEDVEVTNENTDEKVLKDNEHSPLLFAKRRKDKEEDVETT
                     TSIESKDEDVPLLLAKKKNQKDNQSKDKKSASKNTSKKVAAKKKKKKAKKNKK"
     gene            complement(36978..37379)
                     /locus_tag="O0293_000006"
     CDS             complement(36978..37379)
                     /locus_tag="O0293_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HCY0278922.1"
                     /translation="MAKINFDAATKGNPGISTCAIVIKEDEQHYTYTHELGEMDNHTA
                     EWAACIYALEHARELNVQNALLYTDSKLIADSIEAGYVKNANFKPYFDQIEIFEKDFD
                     LLFVKWIPREQNKEANQHAQQALYKLIKKNK"
     gene            complement(37920..38624)
                     /locus_tag="O0293_000007"
     CDS             complement(37920..38624)
                     /locus_tag="O0293_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HCY0278923.1"
                     /translation="MYNEILGLVTFIATFVLMVLMYRFFGKQGLIAWVAIGTIIANIQ
                     VIKTVEIFGISATLGNVMFASIYLATDILNDIYGRRVAKRAVWLGFSSTLIMIIVMQL
                     SLHFIPAPEDMAQKALHAIFDVVPRIALGSIVAYIIGQHIDVFIFSLIKKVFSSDKTF
                     FIRAYGSTFLSSIIDTALFVAIAFIGSLPGTVVFEIFITTYVLKLASTVFNVPFGYIA
                     KSFYRKGKIDKLDQGY"
     gene            38869..39063
                     /locus_tag="O0293_000008"
     CDS             38869..39063
                     /locus_tag="O0293_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HCY0278924.1"
                     /translation="MSRILTFSEQNALAKIDDLMNTYCNQCPIKTRLRKLEGKTKAHH
                     FCINECSIGKEIKQLGNELQ"
     gene            39075..39326
                     /locus_tag="O0293_000009"
     CDS             39075..39326
                     /locus_tag="O0293_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HCY0278925.1"
                     /translation="MKIISISETPNHNTMKITLSESREGMTSDTYTKVDDSQPAFIND
                     ILKVEGVKSIFHVMDFISVDKENDANWETVLPKVEAVFE"
     gene            39364..40488
                     /locus_tag="O0293_000010"
     CDS             39364..40488
                     /locus_tag="O0293_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor C"
                     /protein_id="HCY0278926.1"
                     /translation="MEILRIEPTPSPNTMKVVLSYTREDKLSNTYKKVEETQPRFINQ
                     LLSIDGITSIFHVMNFLAVDKAPKADWEVILPDIKAAFSDANKVLESVNEPQIDNHFG
                     EIKAELLTFKGIPYQIKLTSADQELREQLPQTYVDHMTQAQTAHDNIVFMRKWLDLGN
                     RYGNIQEVMDGVLEEVLTTYPESQLPVLVKHALEENHATNNYHFYRHVSLDEYHATDN
                     WKTRLRMLNHFPKPTFEDIPLLDLALSDEKVPVRRQAIVLLGMIESKEILPYLYKGLR
                     DKSPVVRRTAGDCISDLGYPEALPEMVLLLDDPQKIVRWRAAMFIFDEGNAEQLPALK
                     AHINDNAFEVKLQIEMAISRIENGDEALGSVWKQMANRTI"
     gene            40504..40941
                     /locus_tag="O0293_000011"
     CDS             40504..40941
                     /locus_tag="O0293_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HCY0278927.1"
                     /translation="MNAYDAYMKEIAQQMRGELTQNGFTSLETSEAVSEYMNQVNADD
                     TTFVVINSTCGCAAGLARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQAP
                     SSPSYALFKGQDLVYFMPREFIEGRDINDIAMDLKDAFDENCK"
     gene            41365..42321
                     /locus_tag="O0293_000012"
     CDS             41365..42321
                     /locus_tag="O0293_000012"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCY0278928.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYKGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            42521..43000
                     /locus_tag="O0293_000013"
     CDS             42521..43000
                     /locus_tag="O0293_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCY0278929.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            43015..43854
                     /gene="fakB2"
                     /locus_tag="O0293_000014"
     CDS             43015..43854
                     /gene="fakB2"
                     /locus_tag="O0293_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HCY0278930.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSIGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            43940..44473
                     /gene="msrA"
                     /locus_tag="O0293_000015"
     CDS             43940..44473
                     /gene="msrA"
                     /locus_tag="O0293_000015"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCY0278931.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            44466..44894
                     /gene="msrB"
                     /locus_tag="O0293_000016"
     CDS             44466..44894
                     /gene="msrB"
                     /locus_tag="O0293_000016"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCY0278932.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMLRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            44906..45406
                     /locus_tag="O0293_000017"
     CDS             44906..45406
                     /locus_tag="O0293_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCY0278933.1"
                     /translation="MFKKLFGKGKEVQKDIAIYAPLTGEFVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEYINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            45406..>45591
                     /locus_tag="O0293_000018"
     CDS             45406..>45591
                     /locus_tag="O0293_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCY0278934.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDD"
BASE COUNT        17833 a         7756 c         8240 g        11762 t
ORIGIN      
        1 atagataaaa tatgaataat taattaatgg tatttacata ttcataacca aaggataaaa
       61 taattgtgaa attattcaca aatgaaagag gagtgttgca aatgttagtt gcagtagtca
      121 aagaattaaa acaaggtgaa ggacgtgtag cttgcacacc cgaaaatgtg cgtaagttaa
      181 cggatgcagg acataaagta attgttgaaa aaaatgctgg cattggttca ggattttcta
      241 acgatatgta tgaaaaagaa ggcgctaaga tcgtaactca cgaacaagca tgggaagctg
      301 atcttgttat caaagtaaaa gaacctcatg aaagcgaata tcaatatttc aaaaagaatc
      361 aaattatctg gggattttta catctagcat cttcaaaaga aatagtagaa aaaatgcaag
      421 aagttggtgt aactgcgatt agtggtgaaa ccattataaa aaatggaaaa gcagaattat
      481 tagcgccaat gagtgctata gcaggtcaac gctcagcaat tatgggagct tactactctg
      541 aagcacaaca tggtggtcaa ggtactttag tgactggtgt acatgaaaat gtggatatac
      601 ctggtagtac atatgtgatt ttcggtggtg gagtagcagc aacaaatgca gcaaatgttg
      661 ccttgggact aaatgctaaa gtaatcatta tcgagttaaa cgatgaccgc attaaatatc
      721 ttgaagatat gtatgcagaa aaagatgtca cagtagtcaa atcaacacca gaaaatttag
      781 cagaacaaat taagaaagca gatgtattta tttctacaat tttaattcca ggtgcgaaac
      841 cgccaaaatt ggttactcgt gagatggtta aatcaatgaa aaaaggttca gtattaatcg
      901 atatagctat tgaccaaggt ggaactattg aaacaattag accaactaca atttctgatc
      961 cagtgtatga agaagaaggt gtgattcatt atggtgtacc aaatcaacca ggagcagtcc
     1021 caagaacttc aacaatggca ttagcacaag gaaatattga ttatatatta gaaatttgtg
     1081 acaaaggctt agaacaagca attaaagata atgaagcctt aagtactggt gtaaacattt
     1141 accaaggaca agtgacaaat caaggattag cttcatcaca tgacctagat tataaagaaa
     1201 tattaaatgt tatcgaatag atagtaattt aaatgaaatt gagtgaaatg aatattttaa
     1261 atatagcatt atagtttgga ctaaaaattt acaaaacgga aggatgtaat tattatgaca
     1321 accaacacag ttacattaca aacagcacat attgtaagtt taggagatat cgaagaagct
     1381 aaagcaagca ttaaaccatt tattcgtcga acacctctaa ttaaatcaat gtatttaagc
     1441 caaagtataa ctaaagggaa tgtatttcta aaattagaaa atatgcaatt cacaggatct
     1501 tttaaattta gaggcgctag caataaaatt aatcacttaa cagatgaaca aaaagaaaaa
     1561 ggcattatcg cagcatctgc tgggaaccat gcacaaggtg ttgctttaac agctaaatta
     1621 ttaggcattg atgcaacgat tgtaatgcct gaaacagcac cacaagcgaa acaacaagca
     1681 acaaaaggct atggggcaaa ggttatttta aaaggtaaaa actttaacga aactagactt
     1741 tatatggaag aattagcgaa agaaaatggc atgacaatcg ttcatccata tgacgataag
     1801 tttgtaatgg caggccaagg aacaattggt ttagaaattt tagatgatat ttggaatgtg
     1861 aatacagtca tcgtaccagt tggcggtgga ggattaattg caggtattgc caccgcatta
     1921 aaatcattta acccttcaat tcatattatc ggtgttcaat ctgagaatgt tcatggtatg
     1981 gctgagtctt tctataagag agatttaact gaacatcgag tggatagcac aatagcagat
     2041 ggttgtgatg taaaagttcc tggtgaacaa acatatgaag tagttaaaca tttagtagat
     2101 gaatttattc ttgttactga agaagaaatt gaacatgcta tgaaagattt aatgcagcgt
     2161 gccaaaatta ttactgaagg tgcaggcgca ttaccaacag ctgcaatttt aagtggaaaa
     2221 ataaacaata aatggcttga agataaaaat gttgttgcat tagtttcagg cgggaatgtt
     2281 gacttaacta gagtttcagg tgtcattgaa catggactga atattgcaga tacaagcaag
     2341 ggtgtggtag gttaaaacat ttaatcttaa aaatgaggtg taattatgtc aaatggtaaa
     2401 gaattacaaa aaaatatagg tttcttctca gcgtttgcta ttgttatggg gacagttatt
     2461 ggttcaggag tattctttaa aatatcaaac gtaacagaag taacaggaac agcaggaatg
     2521 gccttgtttg tatggttcct aggcggcatc attaccattt gtgcggggtt aacagcagca
     2581 gaacttgctg ctgcaatccc tgaaacaggt ggcttaacga agtatataga atatacatac
     2641 ggtgatttct ggggcttcct atcaggttgg gcgcaatcat ttatttattt tccagctaac
     2701 gtagcagcat tgtctatcgt atttgcgaca cagctaatta atttattcca tttatctata
     2761 ggttcgttaa taccaatagc aatcgcatct gcgttatcta ttgtgttgat aaatttccta
     2821 ggttcaaaag caggcggaat tttacaatca gttactttag taattaaact gattccaatc
     2881 atcgttattg taatttttgg tatttttcaa tctggagata tcactttttc attaattcca
     2941 actacaggta attcaggaaa tggcttcttt acagcaattg gtagtggttt attagcaact
     3001 atgtttgcat atgatggttg gattcatgta ggaaatgttg cgggggaact taaaaatcct
     3061 aaacgcgatt tacctttagc gatttcagtt ggtatcggtt gtattatggc tgtgtattta
     3121 ttaattaacg ctacattctt attaacgttg ccaatagaac tacttgctgg taatttaaat
     3181 gcagcttcag atacatcaaa aatattattt ggtgaaaatg gcggtaagat tattacaatc
     3241 ggtatattaa tttctgttta tggtacgatc aatggctata ctatgactgg tatgcgcgta
     3301 ccatatgcaa tggctgaaag aaaattattg ccatttagcc atttattcgc aaaattaaca
     3361 aaatctggcg caccatggtt tggcgcaatt atacaactta taatcgctat catcatgatg
     3421 tcaatgggag catttgatac aattacaaat atgttaatct ttgttatttg gttgttctat
     3481 tgtatgtcat ttgttgcggt aataatttta agaaaacgtg aaccaaatat ggaacgacca
     3541 tataaagtac cgttatatcc gatcatacct ttaattgcta ttttggcagg atcatttgta
     3601 ttaattaata cactgtttac acaatttata ttagcaatca ttggaattct aataacagca
     3661 cttggtatac cagtttatta ctataaaaag aaacaaaaag cagcataagg taagataact
     3721 agcattgaga ataaatggat ggactactaa taaatttaaa gttttacaca ttaaaatcaa
     3781 aaaccattca attattctat ggaacagaca aatttctgtt atggaatttg tctgtttttc
     3841 aaaagtatag ggaggcaaat agagatggaa aagccgtcaa gagaggcatt tgaaggcaat
     3901 aataagttgt taataggaat tgttctaagt gtaataacgt tttggctatt tgcacaatca
     3961 ttggttaatg ttgtaccaat acttgaagat agtttcaata cagatattgg aacggttaat
     4021 atcgccgtta gtataactgc tttattttca ggaatgtttg tagtaggagc aggtggtctt
     4081 gctgataaat atggcagaat taaactcacg aacattggta ttatcttaaa tatattaggt
     4141 tcattattaa tcattatttc aaatattcct ttattactta ttataggaag attaattcaa
     4201 ggactttcag cagcatgtat tatgcctgca actttgtcta ttattaagtc atattacatt
     4261 gggaaagata gacaacgcgc tttaagttat tggtcaattg gctcatgggg cggctctggt
     4321 gtttgttcat tttttggagg tgcagttgca acgcttttag gttggcgttg gattttcatc
     4381 ctatcaatta taatttcatt aattgcactg tttcttatta aaggcacacc tgaaactaaa
     4441 tctaaatcga tttctctaaa taaatttgac attaaaggtc tggttctttt agtcattatg
     4501 ctcctcagtt taaatatttt aattactaaa ggatcagaat taggtgtaac ctcacttctt
     4561 tttattactt tattagctat tgcaattgga tcttttagtt tatttatagt tcttgaaaag
     4621 cgtgctacaa atcctttaat cgattttaaa ttatttaaaa ataaagctta cacaggtgca
     4681 acagcttcaa actttttgtt aaatggtgtt gcaggaacat taatagtagc caacacattt
     4741 gttcaaagag gtttaggata ttcttcattg caagcaggaa gtttatcaat cacttattta
     4801 gtaatggtac taattatgat tcgtgttggt gaaaagttac ttcaaacact cggatgcaag
     4861 aaaccaatgt taattggaac aggagttctt attgtcggag aatgtctcat ttcattaact
     4921 ttcttgccag aaatattcta tgtcatttgt tgtattatag gttatttatt ctttggttta
     4981 ggactaggga tatatgctac accatcaaca gatacagcaa ttgcaaatgc accgttagaa
     5041 aaagtaggcg ttgctgcagg tatctataaa atggcttctg cattaggtgg agcatttggc
     5101 gtcgcattga gtggtgcagt atatgcaatc gtatcaaata tgacaaacat ttatacaggt
     5161 gcaatgattg cattatggtt aaatgcaggt atgggaatat tatcattcgt tatcattttg
     5221 ttacttgtgc ctaaacaaaa cgatactcaa ttatgataat tgagaattaa attgaaatca
     5281 tacaagtcgc tacaatatta aacaaaaata taaaccgatt cttatgtgtc attattttaa
     5341 atgaacatag ggattggttt tttattactc ttttacgcta ctttatttat aattattata
     5401 aattgtcaca aattcaattt accttacaat atattttgtg ttattatatt ctggagcata
     5461 aataaattgt tcaacacata gttgtaatgt gtttcaatac tttttggata gattgcgaaa
     5521 ttgtattgaa tcgtcatcgt tttaaatttt taaatgagaa tggaatgagc attacaatac
     5581 acaagcaatc aaaagtaaat acattcacaa cacaacagag acataacaac aagataagga
     5641 gtgaacaata gctgtgaatt atcgtgataa aattcaaaag tttagtattc gtaaatatac
     5701 agttggtaca ttttcaactg tcattgcgac attggtattt ttaggattca atacatcaca
     5761 agcacatgct gctgaaacaa atcaaccagc aagcgtggtt aaacagaaac aacaaagtaa
     5821 taatgaacag actgagaatc gagaatctca agtacaaaat tctcaaaatt cacaaaatgg
     5881 tcaatcatta tctgctactc atgaaaatga gcaaccaaat attagtcaag ctaatttagt
     5941 agatcaaaaa gtagcgcaat catctactac taatgatgaa caaccagcat ctcaaaatgt
     6001 aaatacaaag aaagattcgg caacggctgc gacaacacaa ccagataaag aacaaagtaa
     6061 gcataaacaa aacgaaagtc aatctgctaa taaaaatgga aacgacaata gagcggctca
     6121 tgtagaaaat catgaagcaa atgtagtaac agcttcagat tcatctgata atggtaacgt
     6181 acaacatgac cgaaatgaat tacaagcgtt ttttgatgca aattatcatg attatcgctt
     6241 tattgaccgt gaaaatgcag attctggcac atttaactat gtaaaaggca tttttgataa
     6301 gattaatacg ttattaggca gtaatgatcc aataaacaat aaagacttgc aacttgcata
     6361 caaagaattg gaacaagctg ttgctttaat tcgtacaatg cctcaacgtc aacagactag
     6421 ccgacgttca aatagaattc aaacgcgttc ggttgagtca agagctgcag agcctagatc
     6481 agtatcagac tatcaaaatg caaattcatc atattatgtt gaaaatgcta atgatggttc
     6541 gggctatcct gttggtacat atatcaatgc ttctagtaaa ggggcgccat ataatttacc
     6601 aactacacca tggaatacat tgaaggcctc tgactcaaag gaaattgctc ttatgacagc
     6661 gaaacaaact ggagacgggt accaatgggt tattaagttt aataaaggac atgctccaca
     6721 tcaaaatatg atcttttggt ttgcattacc agcagaccaa gtgccagtag gaagaactga
     6781 ctttgtaaca gttaattcag atggaacaaa tgtacaatgg agtcatggag caggagcagg
     6841 tgcaaataaa ccacttcaac aaatgtggga atatggagta aatgatcctc atcgttcaca
     6901 tgactttaaa ataagaaata gaagtggcca agtaatatat gactggccaa ctgtccatat
     6961 ttattcttta gaagatttat ctagagcgag tgattatttt agtgaagctg gagcgacacc
     7021 tgctactaaa gcttttggta gacaaaattt tgaatatatt aatggtcaaa aacctgctga
     7081 atcaccgggt gttcctaaag tttatacttt catcggtcaa ggtgatgcaa gttatacaat
     7141 ttcatttaaa acacaaggtc caactgttaa taaattgtac tatgcagcag gtgggcgtgc
     7201 tttagagtac aatcaattat ttatgtacag tcaactatac gtcgaatcaa cgcaagacca
     7261 tcaacaacgt cttaatggtt taagacaagt ggttaatcgt acatatcgca taggtacaac
     7321 taaacgtgta gaagtgagtc aaggaaatgt acaaacgaaa aaggtattag aaagtacaaa
     7381 cctaaatata gatgattttg ttgatgatcc tttaagttat gttaagacgc cgagtaataa
     7441 agtgttagga ttttattcga ataatgcaaa tactaatgct tttagaccgg gtggagccca
     7501 acaattaaat gaatatcaat taagtcaatt atttactgat caaaaattac aagaagcagc
     7561 aagaactaga aacccaataa gattaatgat tggtttcgac tatcctgatg cttatggtaa
     7621 tagtgaaact ttagttcctg ttaacttaac ggtattacct gaaatccaac ataatattaa
     7681 attctttaaa aatgacgata ctcaaaatat tgctgaaaaa ccattttcaa aacaagctgg
     7741 gcatccagtt ttctatgtat atgcaggtaa ccaagggaat gcttccgtga atttaggtgg
     7801 tagcgtaaca tctattcaac cattacgtat taatttaaca agtaatgaga attttacaga
     7861 taaagattgg caaattacag gtattccgcg tacattacac attgaaaact cgacaaatag
     7921 acctaataat gccagagaac gcaatattga acttgttggt aacttattac caggggatta
     7981 ctttggaacg atacgttttg gacgtaaaga acaattattc gaaattcgtg ttaaaccaca
     8041 tacaccaaca attacaacga cagctgagca attaagaggt acagcattac aaaaagtgcc
     8101 tgttaatatt tcgggaatac cgttggatcc atcggcattg gtttatttag ttgcaccaac
     8161 aaatcaaact acgaatggtg gtagtgaggc agatcaaata ccatctggtt atacgatact
     8221 tgcgactggt acacctgatg gggtgcataa tacaattact atacgaccgc aagattatgt
     8281 tgtattcata ccacctgtag gtaaacaaat tagagcagta gtttattata ataaagtagt
     8341 tgcatctaat atgagtaatg ctgttactat tttgccagat gacattccac caacaatcaa
     8401 taatcctgtt ggaataaatg ccaaatacta tcgaggcgac gaagtcaact ttacaatggg
     8461 tgtctctgat agacattctg gtataaaaaa tacaactatt acgacattgc cgaatggttg
     8521 gacatcaaat ttaacaaaag cggataagaa taatggctca ttatctatta caggtagagt
     8581 gagtatgaat caggcattta acagtgatat tacatttaaa gtgtcagcga cagacaatgt
     8641 caataatacg acaaatgata gtcaatctaa acatgtttca attcatgtag gtaaaattag
     8701 tgaagatgct catccgattg tattaggaaa tactgagaaa gttgtagtag tcaatccgac
     8761 tgctgtatct aatgatgaaa agcaaagcat aattactgcc tttatgaata aaaaccaaaa
     8821 tataagagga tatttagcat caactgatcc agtaactgtc gataataatg gtaatgtcac
     8881 attacattac cgtgatggct catcgacaac gcttgatgct acaaatgtga tgacatacga
     8941 accagttgtg aaacctgaat accaaactgt caatgctgct aaaacagcaa cggtaacgat
     9001 tgctaaagga caatcattta gtattggtga tattaaacaa tattttactt taagtaatgg
     9061 acaacctatt ccaagtggca catttacaaa tattacatct gatagaacta ttccaactgc
     9121 acaagaagtt agtcaaatga acgcaggcac gcagttatac catataactg ctacaaatgc
     9181 gtatcataaa gatagtgaag acttctatat tagtttgaaa atcatcgatg tgaaacaacc
     9241 agaaggcgat caacgtgtat atcgtacatc aacatatgat ttaactactg atgaaatctc
     9301 aaaagtaaaa caagcattta ttaatgcaaa tagagatgta attacgcttg ccgaaggtga
     9361 tatttcagtt acaaatacac ctaatggtgc taatgtaagt actattacag taaatattaa
     9421 taaaggtcga ttaacgaaat cattcgcgtc aaacctagct aatatgaatt tcttgcgttg
     9481 ggttaatttc ccacaagatt atacagtgac atggacgaat gcaaaaattg caaacagacc
     9541 aacagatggt ggtttatcat ggtctgatga ccataaatct ttaatttatc gttatgatgc
     9601 tacattaggt actcaaatta cgacgaatga tattttaaca atgttaaaag caacaactac
     9661 agtgcctgga ttgcgaaata acattactgg taatgaaaaa tcacaagcag aagctggcgg
     9721 aagacctaac tttagaacga ctggttattc acaatcaaat gcgacaactg atggtcaacg
     9781 tcaatttacg ttgaatggtc aagtgattca agtgttagac atcatcaacc cttcaaacgg
     9841 ttatggtggg caacctgtta caaattcaaa tactcgtgca aaccatagta actcaactgt
     9901 tgttaacgta aacgaaccgg cagctaatgg tgctggcgca tttacaattg accacgttgt
     9961 aaaaagtaat tctacacata atgcaagtga tgcagtttat aaagcacagt tatacttaac
    10021 gccatatggt ccaaaacaat atgttgaaca tttaaatcaa aatacaggaa atactactga
    10081 cgctattaac atttattttg taccaagtga cttagtgaat ccaacaattt cagtaggtaa
    10141 ttacactaat catcaagtgt tctcaggtga aacatttaca aatactatta cagcgaatga
    10201 taactttggt gtgcaatctg taactgtacc aaatacatca caaattacag gtactgttga
    10261 taataaccat caacatgttt ctgcaacggc accaaatgtg acatcagcaa ctaataagac
    10321 aatcaattta ttagcaactg atacaagcgg caatacagct acaacttcgt tcaatgtaac
    10381 agtgaaacct ttgcgtgata aatatcgagt tggtacttca tcaacggctg ctaatcctgt
    10441 gagaattgcc aatatttcga ataatgcgac agtatcacaa gctgatcaaa cgacaattat
    10501 taattcgtta acgtttactg aaacagtacc aaatagaagt tatgcaagag caagtgcgaa
    10561 tgaaatcact agtaaaacag ttagtaatgt cagtcgtact ggaaataatg ccaatgtcac
    10621 agtaactgtt acttatcaag atggaacaac atcaacagtg actgtacctg taaagcatgt
    10681 cattccagaa atcgttgcac attcgcatta cactgtacaa ggccaagact tcccagcagg
    10741 taatggttct agtgcatcag attactttaa gttatctaat ggtagtgaca ttgcagatgc
    10801 aactattaca tgggtaagtg gacaagcgcc aaataaagat aatacacgta ttggtgaaga
    10861 tataactgta actgcacata tcttaattga tggcgaaaca acgccgatta cgaaaacagc
    10921 aacatataaa gtagtaagaa ctgtaccgaa acatgtcttt gaaacagcca gaggtgtttt
    10981 atacccaggt gtttcagata tgtatgatgc gaaacaatat gttaagccag taaataattc
    11041 ttggtcgaca aatgcgcaac atatgaattt ccaatttgtt ggaacatatg gtcctaacaa
    11101 agatgttgta ggcatatcta ctcgtcttat tagagtgaca tatgataata gacaaacaga
    11161 agatttaact attttatcta aagttaaacc tgacccacct agaattgacg caaactctgt
    11221 gacatataaa gcaggtctta caaaccaaga aattaaagtt aataacgtat taaataactc
    11281 gtcagtaaaa ttatttaaag cagataatac accattaaat gtcacaaata ttactcatgg
    11341 tagcggtttt agttcggttg tgacagtaag tgacgcgtta ccaaatggcg gaattaaagc
    11401 aaaatcttca atttcaatga acaatgtgac gtatacgacg caagacgaac atggtcaagt
    11461 tgttacagta acaagaaatg aatctgttga ttcaaatgac agtgcaacag taacagtgac
    11521 accacaatta caagcaacta ctgaaggcgc tgtatttatt aaaggtggcg acggttttga
    11581 tttcggacac gtagaacgat ttattcaaaa cccgccacat ggggcaacgg ttgcatggca
    11641 tgatagtcca gatacatgga agaatacagt cggtaacact cataaaactg cggttgtaac
    11701 attacctaat ggtcaaggta cgcgtaatgt tgaagttcca gtcaaagttt atccagttgc
    11761 taatgcaaag gcgccatcac gtgatgtgaa aggtcaaaat ttgactaatg gaacggatgc
    11821 gatgaactac attacatttg atccaaatac aaacacaaat ggtatcactg cagcatgggc
    11881 aaatagacaa caaccaaata accaacaagc aggcgtgcaa catttaaatg tcgatgtcac
    11941 atatccaggt atttcagctg ctaaacgagt tcctgttact gttaatgtat atcaatttga
    12001 attccctcaa actacttata cgacaacggt tggaggcact ttagcaagtg gtacgcaagc
    12061 atcaggatat gcacatatgc aaaatgctac tggtttacca acagatggat ttacgtataa
    12121 atggaatcgt gatactacag gtacaaatga cgcaaactgg tcagctatga ataaaccgaa
    12181 tgtggctaaa gtcgttaacg caaaatatga cgtcatctat aacggacata cttttgcaac
    12241 atctttacca gcgaaatttg tagtaaaaga tgtgcaacca gcgaaaccaa ctgtgactga
    12301 aacagcggca ggagcgatta caattgcacc tggagcaaac caaacagtga atacacatgc
    12361 cggtaacgta acgacatacg ctgataaatt agttattaaa cgtaatggta acgttgtgac
    12421 gacatttaca cgtcgcaata atacgagtcc atgggtgaaa gaagcatctg cagcaactgt
    12481 agcaggtatt gctggaacta ataatggtat tactgttgca gcaggtactt tcaaccctgc
    12541 tgatacaatt caagttgttg caacgcaagg aagcggagag acagtgagtg atgagcaacg
    12601 tagtgatgat ttcacagttg tcgcaccaca accgaaccaa gcgactacta agatttggca
    12661 aaatggtcat attgatatca cgcctaataa tccatcagga catttaatta atccaactca
    12721 agcaatggat attgcttaca ctgaaaaagt gggtaatggt gcagaacata gtaagacaat
    12781 taatgttgtt cgtggtcaaa ataatcaatg gacaattgcg aataagcctg actatgtaac
    12841 gttagatgca caaactggta aagtgacgtt caatgccaat actataaaac caaattcatc
    12901 aatcacaatt actccgaaag caggtacagg tcactcagta agtagtaatc caagtacatt
    12961 aactgcaccg gcagctcata ctgtcaacac aactgaaatt gtgaaagatt atggttcaaa
    13021 tgtaacagca gctgaaatta acaatgcagt tcaagttgct aataaacgta ctgcaacgat
    13081 taaaaatggc acagcaatgc ctactaattt agctggtggt agcacaacga cgattcctgt
    13141 gacagtaact tacaatgatg gtagtactga agaagtacaa gagtccattt tcacaaaagc
    13201 ggataaacgt gagttaatca cagctaaaaa tcatttagat gatccagtaa gcactgaagg
    13261 taaaaagcca ggtacaatta cgcagtacaa taatgcaatg cataatgcgc aacaacaaat
    13321 caatactgcg aaaacagaag cacaacaagt gattaataat gagcgtgcaa caccacaaca
    13381 agtttctgac gcactaacta aagttcgtgc agcacaaact aagattgatc aagctaaagc
    13441 attacttcaa aataaagaag ataatagcca attagtaacg tctaaaaata acttacaaag
    13501 ttctgtgaac caagtaccat caactgctgg tatgacgcaa caaagtattg ataactataa
    13561 tgcgaagaag cgtgaagcag aaactgaaat aactgcagct caacgtgtta ttgacaatgg
    13621 cgatgcaact gcacaacaaa tttcagatga aaaacatcgt gtcgataacg cattaacagc
    13681 attaaaccaa gcgaaacatg atttaactgc agatacacat gccttagagc aagcagtgca
    13741 acaattgaat cgcacaggta caacgactgg taagaagccg gcaagtatta ctgcttacaa
    13801 taattcgatt cgtgcacttc aaagtgactt aacaagtgct aaaaatagcg ctaatgctat
    13861 tattcaaaag ccaataagaa cagtacaaga agtgcaatct gcgttaacaa atgtaaatcg
    13921 tgtcaatgag cgattaacgc aagcaattaa tcaattagta cctttagctg ataatagtgc
    13981 tttaaaaact gctaagacga aacttgatga agaaatcaat aaatcagtaa ctactgatgg
    14041 tatgacacaa tcatcaatcc aagcatatga aaatgctaaa cgtgcgggtc aaacagaatc
    14101 aacaaatgca caaaatgtta ttaacaatgg tgatgcgact gaccaacaaa ttgccgcaga
    14161 aaaaacaaaa gtagaagaaa aatataatag cttaaaacaa gcaattgctg gattaactcc
    14221 agacttggca ccattacaaa ctgcaaaaac tcagttgcaa aatgatattg atcagccaac
    14281 gagtacgact ggtatgacaa gcgcatctat tgcagcattt aatgaaaaac tttcagcagc
    14341 tagaactaaa attcaagaaa ttgatcgtgt attagcctca catccagatg ttgcgacaat
    14401 acgtcaaaac gtgacagcag cgaatgccgc taaatcagca cttgatcaag cacgtaatgg
    14461 cttaacagtc gataaagcgc ctttagaaaa tgcgaaaaat caactacaac atagtattga
    14521 cacgcaaaca agtacaactg gtatgacaca agactctata aatgcataca atgcgaagtt
    14581 aacagctgca cgtaataaga ttcaacaaat caatcaagta ttagcaggtt caccgactgt
    14641 agaacaaatt aatacaaata cgtctacagc aaatcaagct aaatctgatt tagatcatgc
    14701 acgtcaagct ttaacaccag ataaagcgcc gcttcaaact gcgaaaacgc aattagaaca
    14761 aagcattaat caaccaacgg atacaacagg tatgacgacc gcttcgttaa atgcgtacaa
    14821 ccaaaaatta caagcagcgc gtcaaaagtt aactgaaatt aatcaagtgt tgaatggcaa
    14881 cccaactgtc caaaatatca atgataaagt gacagaggca aaccaagcta aggatcaatt
    14941 aaatacagca cgtcaaggtt taacattaga tagacagcca gcgttaacaa cattacatgg
    15001 tgcatctaac ttaaaccaag cacaacaaaa taatttcacg caacaaatta atgctgctca
    15061 aaatcatgct gcgcttgaaa caattaagtc taacattacg gctttaaata ctgcgatgac
    15121 gaaattaaaa gacagtgttg cggataataa tacaattaaa tcagatcaaa attacactga
    15181 cgcaacacca gctaataaac aagcgtatga taatgcagtt aatgcggcta aaggtgtcat
    15241 tggagaaacg actaatccaa cgatggatgt taacacagtg aaccaaaaag cagcatctgt
    15301 taaatcgacg aaagatgctt tagatggtca acaaaactta caacgtgcga aaacagaagc
    15361 aacaaatgcg attacgcatg caagtgattt aaaccaagca caaaagaatg cattaacaca
    15421 acaagtgaat agtgcacaaa acgtgcaagc agtaaatgat attaaacaaa cgactcaaag
    15481 cttaaatact gctatgacag gtttaaaacg tggcgttgct aatcataacc aagtcgtaca
    15541 aagtgataat tatgtcaacg cagatactaa taagaaaaat gattacaaca atgcatacaa
    15601 ccatgcgaat gacattatta atggtaatgc acaacatcca gttataacac caagtgatgt
    15661 taacaatgct ttatcaaatg tcacaagtaa agaacatgca ttgaatggtg aagctaagtt
    15721 aaatgctgcg aaacaagaag cgaatactgc attaggtcat ttaaacaatt taaataatgc
    15781 acaacgtcaa aacttacaat cgcaaattaa tggtgcgcat caaattgatg cagttaatac
    15841 aattaagcaa aatgcaacaa acttgaatag tgcaatgggt aacttaagac aagctgttgc
    15901 agataaagat caagtgaaac gtacagaaga ttatgcggat gcagatacag ctaaacaaaa
    15961 tgcatataac agtgcagttt caagtgccga aacaatcatt aatcaaacaa caaatccaac
    16021 gatgtctgtt gatgatgtta atcgtgcaac ttcagctgtt acttctaata aaaatgcatt
    16081 aaatggttat gaaaaattag cacaatctaa aacagatgct gcaagagcaa ttgatgcatt
    16141 accacattta aataatgcac aaaaagcaga tgttaaatct aaaattaatg ctgcatcaaa
    16201 tattgctggc gtaaatactg ttaaacaaca aggtacagat ttaaatacag cgatgggtaa
    16261 cttgcaaggt gcaatcaatg atgaacaaac gacgcttaat agtcaaaact atcaagatgc
    16321 gacacctagt aagaaaacag catacacaaa tgcggtacaa gctgcgaaag atattttaaa
    16381 taaatcaaat ggtcaaaata aaacgaaaga tcaagttact gaagcgatga atcaagtgaa
    16441 ttctgctaaa aataacttag atggtacgcg tttattagat caagcgaagc aaacagcaaa
    16501 acagcagtta aataatatga cgcatttaac aactgcacaa aaaacgaatt taacaaacca
    16561 aattaatagt ggtactactg tcgctggtgt tcaaacggtt caatcaaatg ccaatacatt
    16621 agatcaagcc atgaatacgt taagacaaag tattgccaac aaagatgcga ctaaagcaag
    16681 tgaagattac gtagatgcta ataatgataa gcaaacagca tataacaacg cagtagctgc
    16741 tgctgaaacg attattaatg ctaatagtaa tccagaaatg aatccaagta cgattacaca
    16801 aaaagcagag caagtgaata gttctaaaac ggcacttaac ggtgatgaaa acttagctgc
    16861 tgcaaaacaa aatgcgaaaa cgtacttaaa cacattgaca agtattacag atgctcaaaa
    16921 gaacaatttg attagtcaaa ttactagtgc gacaagagtg agtggtgttg atactgtaaa
    16981 acaaaatgcg caacatctag accaagctat ggctagctta cagaatggta ttaacaacga
    17041 atctcaagtg aaatcatctg agaaatatcg tgatgctgat acaaataaac aacaagagta
    17101 tgataatgct attactgcag cgaaagcgat tttaaataaa tcgacaggtc caaacactgc
    17161 gcaaaatgca gttgaagcag cattacaacg tgttaataat gcgaaagatg cattgaatgg
    17221 tgatgcaaaa ttaattgcag ctcaaaacgc agcgaaacaa catttaggta ctttaacgca
    17281 tatcactaca gctcaacgta atgatttaac aaatcaaatt tcacaagcta caaacttagc
    17341 tggtgttgaa tctgttaaac aaaatgcgaa tagtttagat ggtgctatgg gtaacttaca
    17401 aacggctatc aacgataagt caggaacatt agcgagccaa aacttcttgg atgctgatga
    17461 gcaaaaacgt aatgcataca atcaagctgt atcagcagcc gaaaccattt taaataaaca
    17521 aactggaccg aatacagcga aaacagcagt cgaacaagca cttaataatg ttaataatgc
    17581 gaaacatgca ttaaatggta cgcaaaactt aaacaatgcg aaacaagcag cgattacagc
    17641 aatcaatggc gcatctgatt taaatcaaaa acaaaaagat gcattaaaag cacaagctaa
    17701 tggtgctcaa cgcgtatcta atgcacaaga tgtacagcac aatgcgactg aactgaacac
    17761 ggcaatgggc acattaaaac atgccatcgc agataagacg aatacgttag caagcagtaa
    17821 atatgttaat gccgatagca ctaaacaaaa tgcttacaca actaaagtta ccaatgctga
    17881 acatattatt agcggtacgc caacggttgt tacgacacct tcagaagtaa cagctgcagc
    17941 taatcaagta aacagcgcga aacaagaatt aaatggtgac gaaagattac gtgaagcaaa
    18001 acaaaacgcc aatactgcta ttgatgcatt aacacaatta aatacacctc aaaaagctaa
    18061 attaaaagaa caagtgggac aagccaatag attagaagac gtacaaactg ttcaaacaaa
    18121 tggacaagca ttgaacaatg caatgaaagg cttaagagat agtattgcta acgaaacaac
    18181 agtcaaaaca agtcaaaact atacagacgc aagtccgaat aaccaatcaa catataatag
    18241 cgctgtgtca aatgcgaaag gtatcattaa tcaaactaac aatccgacta tggatactag
    18301 tgcgattacc caagctacaa cacaagtgaa taatgctaaa aatggtttaa acggtgctga
    18361 aaacttaaga aatgcacaaa acactgctaa gcaaaactta aatacattat cacacttaac
    18421 aaataaccaa aaatctgcca tctcatcaca aattgatcgt gcaggtcatg tgagtgaggt
    18481 aactgctact aaaaatgcag caactgagtt gaatacgcaa atgggtaact tggaacaagc
    18541 tatccatgat caaaacacag ttaaacaaag tgttaaattt actgatgcag ataaagctaa
    18601 acgtgatgcg tatacaaatg cggtaagcag agctgaagca attctgaata aaacgcaagg
    18661 tgcaaatacg tctaaacaag atgttgaagc ggctattcaa aatgtttcaa gtgctaaaaa
    18721 tgcattgaat ggtgatcaaa acgttacaaa tgcgaagaat gcagctaaaa atgcattaaa
    18781 taacttaacg tcaattaata atgcacaaaa acgtgactta acaactaaaa ttgatcaagc
    18841 aacaactgta gctggtgttg aagctgtatc taatacgagt acacaattga atacagcgat
    18901 ggctaacttg caaaatggta ttaatgataa aacaaataca ctagcaagtg aaaactatca
    18961 tgatgctgat tcagataaga aaactgctta tactcaagcc gttacgaacg cagaaaatat
    19021 tttaaataaa aatagtggat caaatttaga caaaactgcc gttgaaaacg cgttgtcaca
    19081 agttgctaat gcgaaaggtg ccctaaatgg taaccataat ttagagcaag ctaaatcaaa
    19141 tgcaaacact actataaacg gacttcaaca tttaacaact gctcaaaaag ataaattgaa
    19201 acaacaagtg caacaagcac aaaatgttgc aggtgtagat actgttaaat caagtgccaa
    19261 cacattaaat ggtgctatgg gtacgttaag aaatagcata caagataaca cagctacgaa
    19321 aaatggccaa aactatcttg atgctacaga acgtaacaaa acaaactata acaatgctgt
    19381 tgatagtgct aatggtgtca ttaatgcaac aagcaatcca aatatggatg ctaatgcaat
    19441 taaccaaatc gctacacaag tgacatcaac gaaaaatgca ttagatggta cacataattt
    19501 aacgcaagcg aaacaaacag caacaaatgc catcgatggt gctactaact taaataaagc
    19561 gcaaaaagat gcgttaaaag cacaagttac aagtgcgcaa cgtgttgcaa atgtaacaag
    19621 tatccaacaa actgcaaatg aacttaatac agctatgggt caattacaac atggtattga
    19681 tgatgaaaat gcaacaaaac aaactcaaaa atatcgtgac gctgaacaaa gtaagaaaac
    19741 tgcttatgat caagctgtag ctgctgcgaa agcaatttta aataaacaaa caggttcaaa
    19801 ttcagataaa gcagcagttg accgtgcatt acaacaagta acaagtacga aagatgcatt
    19861 gaatggtgat gcaaaactgg cagaagcgaa agcggcagct aaacaaaact taggcacttt
    19921 aaaccatatt acgaatgcac aacgtactga cttagaaggc caaatcaatc aagcgacgac
    19981 tgttgatggc gttaatactg taaaaacaaa tgccaataca ttagacggcg caatgaatag
    20041 cttacaaggt tcaatcaatg ataaagatgc gacattaaga aatcaaaatt atcttgatgc
    20101 ggatgaatca aaacgaaatg catatacgca agctgtcaca gcggctgaag gcattttaaa
    20161 taaacaaact ggtggtaaca catctaaagc agacgttgat aatgcattaa atgcagttac
    20221 aagagcgaaa gcggctttaa atggtgctga caacttaaga aatgcgaaaa cttcagcaac
    20281 aaatacgatt gatggtttac ctaacttaac acaattacaa aaagacaact tgaagcatca
    20341 agttgaacaa gcgcaaaatg tagcaggtgt aaatggtgtt aaagataaag gtaatacgtt
    20401 aaatactgcc atgggtgcat tacgtacaag tatccaaaat gataatacga cgaaaacaag
    20461 tcaaaattat cttgatgcat ctgacagcaa caaaaataat tacaatactg ctgtaaataa
    20521 tgcaaatggt gttattaatg caacgaacaa tccaaatatg gatgctaatg cgattaatgg
    20581 catggcaaat caagtcaata caacaaaagc agcgttaaat ggtgcacaaa acttagctca
    20641 agctaaaaca aatgcgacga acacaattaa caacgcacat gacttaaacc aaaaacaaaa
    20701 agatgcatta aaaacacaag ttaacaatgc acaacgtgta tctgatgcaa ataacgttca
    20761 acacactgca actgaattga acagtgcgat gacagcactt aaagcagcta ttgctgataa
    20821 agaaagaaca aaagcaagcg gtaattatgt caatgctgat caagaaaaac gtcaagcgta
    20881 tgattcaaaa gtgactaacg ctgaaaatat cattagtggt acaccgaatg cgacattaac
    20941 agtcaatgac gtaaatagtg cggcatcaca agtcaatgcg gctaaaacag cattaaatgg
    21001 tgataacaac ttacgtgtag cgaaagagca tgccaacaat acaattgacg gcttagcaca
    21061 attgaataat gcacaaaaag caaaattaaa agaacaagtt caaagtgcaa ctacattaga
    21121 tggtgttcaa actgttaaaa atagttctca aacgttgaat acagcgatga aaggcttaag
    21181 agatagtatt gcgaatgaag caacaattaa agcaggtcaa aactacactg acgcaagtcc
    21241 aaataatcgt aacgagtacg acagtgcagt tactgcagca aaagcaatca ttaatcaaac
    21301 atcgaaccca acgatggaac caaatactat tacgcaagta acatcacaag tgacaactaa
    21361 agaacaggca ttaaatggtg cgcgaaactt agctcaagct aagacaactg cgaaaaacaa
    21421 cttgaataac ttaacatcaa ttaacaatgc acaaaaagat gcgttaacgc gtagcattga
    21481 tggtgcaaca acagtagctg gtgtaaatca agaaactgca aaagcaacag aattaaataa
    21541 cgcaatgcat agtttacaaa atggtatcaa tgatgagaca caaacaaaac aaactcagaa
    21601 atacctagat gcagagccaa gtaagaaatc agcttatgat caagcagtaa atgcagcgaa
    21661 agcaatttta acaaaagcta gtggtcaaaa tgtagacaaa gcagcagttg aacaagcatt
    21721 gcaaaatgtg aacagtacga agacggcgtt gaacggtgat gcgaaattaa atgaagctaa
    21781 agcagctgcg aaacaaacgt taggtacatt aacacacatt aataatgcac aacgtacagc
    21841 gttagacaat gaaattacac aagcaacaaa tgttgaaggt gttaatacag ttaaagccaa
    21901 agcgcaacaa ttagatggtg ctatgggtca attagaaaca tcaattcgtg ataaagacac
    21961 gacgttacaa agtcaaaatt atcaagatgc tgatgatgct aaacgaactg cttattctca
    22021 agcagtaaat gcagcagcaa ctattttaaa taaaacagct ggcggtaata cacctaaagc
    22081 agatgttgaa agagcaatgc aagctgttac acaagcaaat actgcattaa acggtattca
    22141 aaacttagat cgtgcgaaac aggctgctaa cacagcgatt acaaatgctt cggacttaaa
    22201 tacaaaacaa aaagaagcat taaaagcaca agtaacaagt gcaggacgtg tatctgcagc
    22261 aaatggtgtt gaacatactg cgactgaatt aaatactgcg atgacagctt taaagcgtgc
    22321 cattgctgat aaagctgaga caaaagctag tggtaactat gtcaatgctg atgcgaataa
    22381 acgtcaagca tatgatgaaa aagttacagc tgccgaaaat atcgttagtg gtacaccaac
    22441 accaacgtta acaccagcag atgttacaaa tgcagcaacg caagtaacga atgctaagac
    22501 gcagttaaac ggtaatcata atttagaagt agcgaaacaa aatgctaaca ctgcaattga
    22561 tggtttaact tctttaaatg gtccgcaaaa agcaaaactt aaagaacaag tgggtcaagc
    22621 gacgacgttg ccaaatgttc aaactgttcg tgataatgca caaacattaa acactgcaat
    22681 gaaaggtcta cgagatagca ttgcgaatga agcaacgatt aaagcaggtc aaaactacac
    22741 agatgcaagt caaaacaaac aaactgacta caacagtgca gtcactgcag caaaagcaat
    22801 cattggtcaa acaactagtc catcaatgaa tgcgcaagaa attaatcaag cgaaagacca
    22861 agtgacagct aaacaacaag cgttaaacgg tcaagaaaac ttaagaactg cgcaaacaaa
    22921 tgcgaagcaa catttgaacg gcttaagtga cttaactgac gctcaaaaag atgcagtgaa
    22981 acgtcaaatc gaaggtgcaa cgcatgttaa tgaagtaaca caagcacaaa ataatgcgga
    23041 tgcattaaat acagctatga cgaacttgaa aaatggtatt caagatcaga atacgattaa
    23101 gcaaggtgtt aacttcactg atgccgacga agcgaaacgt aatgcatata caaatgcagt
    23161 gacgcaagct gaacaaattt taaataaagc acaaggtcca aatacttcaa aagacggtgt
    23221 cgaaactgcg ttagaaaatg tacaacgtgc taaaaacgaa ttgaacggta atcaaaatgt
    23281 tgcgaacgct aagacaactg cgaaaaatgc attgaataac ctaacatcaa ttaataatgc
    23341 acaaaaagaa gcattgaaat cacaaattga aggtgcgaca acagttgcag gtgtaaatca
    23401 agtgtctaca acggcatctg aattaaatac agcaatgagc aacttacaaa atggtattaa
    23461 tgatgaagca gctacaaaag cagctcagaa atatactgat gcagatagag aaaaacaaac
    23521 tgcatacaat gacgctgtaa cagcagctaa aacgttatta gataaaacag ctggttcaaa
    23581 tgacaataaa gcagctgttg aacaagcatt acaacgtgtg aatactgcta aaacagcatt
    23641 aaatggtgac gagcgattaa atgaagcgaa gaacacagct aaacaacaag tagcgacaat
    23701 gtcacactta actgatgctc aaaaagcaaa cttaacatcg caaatcgaaa gtggtacgac
    23761 tgttgcaggt gttcaaggta ttcaagctaa tgccggtact ttagatcaag caatgaatca
    23821 attaagacaa agtattgctt ctaaagatgc gactaaatca agcgaagatt atcaagacgc
    23881 gaatgcagat ttacaaaatg catacaatga tgcggtaact aatgctgaag gtattattag
    23941 tgcaacgaat aaccctgaaa tgaatcctga tacaattaac caaaaagcga gccaagtgaa
    24001 cagtgcgaag tctgcattga acggtgatga aaaattagca gcagcaaaac aaactgcgaa
    24061 atcagatatc ggtcgtttga cagacttgaa caatgcacaa cgaactgcgg caaatgctga
    24121 agtggatcaa gcaccaaatc ttgcagctgt cacagcggct aaaaataaag caacatcgtt
    24181 aaacacagcg atgggtaatt tgaaacatgc acttgctgaa aaggataata cgaaacgtag
    24241 tgtcaattac acagatgcgg atcaaccaaa acaacaagcg tatgatactg cggttacaca
    24301 agcagaagca attactaatg ctaatggcag caacgcgaat gaaacacaag ttcaagcagc
    24361 actaaaccaa ttgaatcaag ctaaaaatga cttgaatggt gataataaag ttgctcaagc
    24421 aaaagagtca gcgaaacgtg cattagcttc atatagtaac ttgaataatg cgcaatcaac
    24481 tgcagcaatt agtcaaattg acaatgcaac gacagtagca ggcgtaactg ctgcacaaaa
    24541 tactgctaat gaattaaata cagcaatggg tcaacttcaa aatggtataa atgaccaaaa
    24601 cactgttaaa caacaagtga actttacaga tgctgaccaa ggtaagaaag atgcttacac
    24661 aaatgctgtt acgaatgctc aaggtatttt agataaagca cacggtcaaa atatgacgaa
    24721 agcacaagtt gaagctgcat taaatcaagt aacgactgct aagaatgctt taaacggtga
    24781 cgcaaatgta agacaagcaa aatcagatgc gaaagcaaac ttaggtacat taacacactt
    24841 aaataatgca caaaaacaag atttaacatc acaaatcgaa ggcgcaacaa cagtcaacgg
    24901 tgtaaatggt gttaaaacga aagcacaaga cttagatggt gcaatgcaac gattacaatc
    24961 agctatagca aataaagatc aaactaaagc gagcgaaaat tacatcgacg cagatccaac
    25021 taagaaaaca gcatttgata atgctatcac acaagctgaa tcttacttaa ataaagatca
    25081 cggtgcgaat aaagataagc aagctgttga acaagcaatt caaagtgtaa cgtctactga
    25141 aaatgctttg aacggtgacg cgaacttaca acgcgctaaa actgaagcta tacaagctat
    25201 cgataacttg acacatttga atacaccaca aaaaacagca ttaaaacaac aagtgaacgc
    25261 tgcgcaacgt gtatcaggtg taactgatct gaaaaatagt gctacatcac ttaataatgc
    25321 gatggatcaa ttaaaacaag caattgctga tcatgacaca attgtagcta gtggtaatta
    25381 cactaacgcg agtcctgata agcaaggtgc ttatactgat gcatataatg ctgcgaaaaa
    25441 cattgtaaat ggttcgccta atgtgattac aaatgcagca gatgttacag cagcaacaca
    25501 acgtgttaat aatgctgaaa caggtttaaa cggtgataca aacttagcaa ctgcgaagca
    25561 acaagctaaa gatgcattac gtcaaatgac acatttatct gatgcacaaa aacaaagtat
    25621 tactggtcaa attgatagcg cgacacaagt aactggcgtt caaagtgtga aagacaacgc
    25681 gacaaatctt gataatgcaa tgaatcaact tcgaaatagt attgcgaata aagatgatgt
    25741 aaaagcgagt caaccatatg ttgatgcaga tagagataaa caaaatgcat acaatacagc
    25801 agttacaaat gctgaaaata tcattaatgc aacgagtcag ccgacacttg atccatctgc
    25861 agtaacacaa gcagctaatc aagtgagcac taacaaaact gcgcttaatg gtgcacaaaa
    25921 cttagcgaat aaaaagcaag aaacgactgc taacatcaac caattaagtc atttaaataa
    25981 tgctcaaaag caagatttaa atacgcaagt gacaaatgca ccaaatatta gcacagtaaa
    26041 tcaagtgaaa actaaagctg aacaattaga tcaagcaatg gaacgtttaa tcaacggaat
    26101 ccaagacaaa gatcaagtga aacaaagtgt taactttaca gatgcagatc cagaaaaaca
    26161 aacagcatac aacaatgcgg taactgctgc tgaaaatatt attaatcaag caaatggtac
    26221 aaatgcgaac caatcacaag ttgaagcagc actttcaact gtaacaacta cgaaacaagc
    26281 gttgaatggt gatagaaaag taacagatgc taaaaacaat gcaaaccaaa cattatctac
    26341 gttagataac ttaaacaatg cacaaaaagg tgctgttact ggaaacatca atcaagcgca
    26401 cactgtagct gaagtaacgc aagccattca aaccgctcag gaactgaata cagcgatggg
    26461 taacttgaaa aatagcttga atgataaaga cactacactt ggcagtcaaa actttgcaga
    26521 tgcagatcca gagaagaaaa atgcatacaa tgaagcggtt cataatgctg aaaatatttt
    26581 aaataaatct acaggtacga acgtgcctaa agatcaagtt gaagcagcta tgaatcaagt
    26641 gaatgctaca aaagcagcgc ttaatggtac tcaaaacctt gaaaaagcta aacaacacgc
    26701 aaatacagca attgacggtt taagccattt aacaaatgca caaaaagagg cattaaaaca
    26761 attggtacaa caatcgacta ctgttgcaga agcacaaggt aatgagcaaa aagcaaacaa
    26821 tgttgatgca gcaatggaca aattacgtca aagtattgca gataatgcga caacaaaaca
    26881 aaaccaaaat tatactgatg caagtcagaa taaaaaggat gcgtacaata atgctgtcac
    26941 aactgcacaa ggtattattg atcaaactac aagtccaact ttagatccga ctgttatcaa
    27001 tcaagctgct ggacaagtaa gcacaactaa aaatgcatta aatggtaatg aaaacctaga
    27061 ggcagcgaaa caacaagcgt cacaatcatt aggttcatta gataacttaa ataatgcgca
    27121 aaaacaaaca gttactgatc aaattaatgg cgcgcatact gttgatgaag caaatcaaat
    27181 taagcaaaat gcgcaaaact taaatacagc gatgggtaac ttgaaacaag cgatagctga
    27241 caaagatgct acgaaagcga cagttaactt cactgatgca gatcaagcaa aacaacaagc
    27301 atataacact gctgttacaa atgctgaaaa tatcatttca aaagctaatg gcggcaatgc
    27361 aacacaagct gaagttgaac aagcaatcaa acaagttaat gctgcaaaac aagcattaaa
    27421 tggtaatgcc aacgttcaac atgcaaaaga cgaagcaaca gcattaatta atagctctaa
    27481 tgaccttaac caagcacaaa aagacgcatt aaaacaacaa gttcaaaatg caactactgt
    27541 agctggtgta aacaatgtta aacaaacagc acaagagtta aacaatgcta tgacacaatt
    27601 aaaacaaggc attgcagata aagaacaaac aaaagctgat ggtaactttg tcaatgcaga
    27661 tcctgataag caaaatgcat ataatcaagc agtagcgaaa gctgaagcat taattagtgc
    27721 tacgcctgat gttgtcgtta cacctagcga aattactgca gcgttaaata aagttacgca
    27781 agctaaaaat gatttaaatg gtaatacaaa cttagcaacg gcgaaacaaa atgttcaaca
    27841 tgctattgat caattgccaa acttaaacca agcgcaacgt gatgaataca gcaaacaaat
    27901 cacgcaagca acacttgtac caaacgtcaa tgctattcaa caagcggcga caacgcttaa
    27961 tgacgcgatg acacaattga aacaaggtat tgcgaataaa gcacaaatta aaggtagcga
    28021 gaactatcac gatgctgata ctgacaagca aacagcatat gataatgcag taacaaaagc
    28081 agaagaattg ttaaaacaaa caacaaatcc aacaatggat ccaaatacaa ttcaacaagc
    28141 attaactaaa gtgaatgaca caaatcaagc acttaacggt aatcaaaaat tagctgatgc
    28201 caaacaagat gctaagacaa cacttggtac actagatcat ttaaatgatg ctcaaaaaca
    28261 agcgctaaca actcaagttg aacaagcacc agatattgca acagttaata atgttaagca
    28321 aaatgctcaa aatctgaata atgctatgac taacttaaac aatgcattac aagataaaac
    28381 tgagacatta aatagcatta actttactga tgcagatcaa gctaagaaag atgcttatac
    28441 taatgcggtt tcacatgcag aaggtatttt atctaaagca aatggcagca atgcaagtca
    28501 aactgaagtg gaacaagcga tgcaacgtgt gaacgaagcg aaacaagcat tgaatggtaa
    28561 tgacaatgta caacgtgcaa aagatgcagc gaaacaagtg attacaaatg caaatgattt
    28621 aaatcaagcg caaaaagatg cattaaaaca acaagtcgat gctgcgcaaa ctgttgcaaa
    28681 tgtaaacacg attaagcaaa cagcacaaga tttaaatcaa gcaatgacac aattgaaaca
    28741 aggtattgca gataaagacc aaactaaagc aaatggtaac tttgtcaatg ctgatactga
    28801 taagcaaaat gcttacaaca atgcggtagc acatgctgaa caaataatta gtggtacacc
    28861 aaatgcaaac gtggatccac aacaagtggc tcaagcgtta caacaagtga atcaagctaa
    28921 gggtgattta aacggtaacc ataacttaca agttgctaaa gacaatgcaa atacagccat
    28981 tgatcagtta ccaaacttaa atcaaccaca aaaaacagca ttaaaagacc aagtgtcgca
    29041 tgcagaactt gttacaggtg ttaatgctat taagcaaaat gctgatgcgt taaataatgc
    29101 aatgggtaca ttgaaacaac aaattcaagc gaacagtcaa gtaccacagt cagttgactt
    29161 tacacaagcg gatcaagaca aacaacaagc atataacaat gcggctaacc aagcgcaaca
    29221 aatcgcaaat ggcataccaa cacctgtatt gacgcctgat acagtaacac aagcagtgac
    29281 aactatgaat caagcgaaag atgcattaaa cggtgatgaa aaattagcac aagcgaaaca
    29341 agaagcttta gcaaatcttg atacgttacg cgatttaaat caaccacaac gtgatgcatt
    29401 acgtaaccaa atcaatcaag cacaagcgtt agctacagtt gaacaaacta aacaaaatgc
    29461 acaaaatgtg aatacagcaa tgagtaactt gaaacaaggt attgcaaaca aagatactgt
    29521 caaagcaagt gagaactatc atgatgctga tgccgataag caaacagcat atacaaatgc
    29581 agtgtctcaa gcggaaggta ttatcaatca aacgacaaat ccaacgctta acccagatga
    29641 aataacacgt gcattaactc aagtgactga tgctaaaaat ggcttaaacg gtgaagctaa
    29701 attggcaact gaaaagcaaa atgctaaaga tgccgtaagt gggatgacgc atttaaacga
    29761 tgctcaaaaa caagcattaa aaggtcaaat cgatcaatcg cctgaaattg ctacagtgaa
    29821 ccaagttaaa caaacagcaa cgagcctaga tcaagcaatg gatcaattat cacaagctat
    29881 taatgataaa gctcaaacat tagcggacgg taattactta aatgcagatc ctgacaaaca
    29941 aaatgcgtat aaacaggcag tagcaaaagc tgaagcatta ttgaataaac aaagtggtac
    30001 taatgaagta caagcacaag ttgaaagcat cactaatgaa gtgaacgcag cgaaacaagc
    30061 attaaatggt aatgacaatt tggcaaatgc aaaacaacaa gcaaaacaac aattggcgaa
    30121 cttaacacac ttaaatgatg cacaaaaaca atcatttgaa agtcaaatta cacaagcgcc
    30181 acttgttaca gatgtcacta cgattaatca aaaagcacaa acgttagatc atgcgatgga
    30241 attattaaga aatagtgttg cggataatca aacgacatta gcgtctgaag attatcatga
    30301 tgcaactgcg caaagacaaa atgactataa ccaagctgta acagctgcta ataatatcat
    30361 taatcaaact acatcgccta cgatgaatcc agatgatgtt aatggtgcaa cgacacaagt
    30421 gaataatacg aaagttgcat tagatggtga tgaaaacctt gcagcagcta aacaacaagc
    30481 aaacaacaga cttgatcaat tagatcattt gaataatgcg caaaagcaac agttacaatc
    30541 acaaattacg caatcatctg atattgctgc agttaatggt cacaaacaaa cagcagaatc
    30601 tttaaatact gcgatgggta acttaattaa tgcgattgca gatcatcaag ccgttgaaca
    30661 acgtggtaac ttcatcaatg ctgatactga taaacaaact gcttataata cagcggtaaa
    30721 tgaagcagca gcaatgatta acaaacaaac tggtcaaaat gcgaaccaaa cagaagtaga
    30781 acaagctatt actaaagttc aaacaacact tcaagcgtta aatggagacc ataatttaca
    30841 agttgctaaa acaaatgcga cgcaagcaat tgatgcttta acaagcttaa atgatcctca
    30901 aaaaacagca ttaaaagacc aagttacagc tgcaacttta gtaactgcag ttcatcaaat
    30961 tgaacaaaat gcgaatacgc ttaaccaagc aatgcatggt ttaagacaga gcattcaaga
    31021 taacgcagca actaaagcaa atagcaaata tatcaacgaa gatcaaccag agcaacaaaa
    31081 ctatgatcaa gctgttcaag ccgcaaataa tattatcaat gaacaaactg caacattaga
    31141 taataatgcg attaatcaag cagcgacaac tgtgaataca acgaaagcag cattacatgg
    31201 tgatgtgaag ttacaaaatg ataaagatca tgctaagcaa acggttagtc aattagcaca
    31261 tctaaacaat gcacaaaaac atatggaaga tacgttaatt gatagtgaaa caactagaac
    31321 agcagttaag caagatttga ctgaagcaca agcattagat caacttatgg atgcattaca
    31381 acaaagtatt gctgacaaag atgcaacacg tgcgagcagt gcatatgtca atgcagaacc
    31441 gaataaaaaa caatcctatg atgaagcagt tcaaaatgct gagtctatca ttgcaggatt
    31501 aaataatcca actatcaata aaggtaatgt atcaagtgcg actcaagcag taatatcatc
    31561 taaaaatgca ttagatggtg ttgaacgatt agctcaagat aagcaaactg ctggaaattc
    31621 tctaaatcat ttagatcaat taacaccagc tcaacaacaa gcgctagaaa atcaaattaa
    31681 taatgcaaca actcgtggcg aagtagcaca aaaattaact gaagcacaag cacttaacca
    31741 agcaatggaa gctttacgta atagcattca agatcaacag caaacggaag cgggtagcaa
    31801 gtttatcaat gaagataaac cacaaaaaga tgcttaccaa gcagcagttc aaaatgcaaa
    31861 agatttaatt aatcaaacta acaatccaac gcttgataaa gcacaagttg aacaattgac
    31921 acaagctgtt aaccaagcta aagataacct acacggtgat caaaaacttg cagacgataa
    31981 acaacatgcg gttactgatt taaatcaatt aaatggtttg aataatccgc aacgtcaagc
    32041 acttgaaagc caaataaaca acgcagcaac tcgtggcgaa gtagcacaaa aattagctga
    32101 agcaaaagcg cttgatcaag caatgcaagc attacgtaat agtattcaag atcaacaaca
    32161 aacagaatct ggtagcaagt ttatcaatga agataaaccg caaaaagatg cttaccaagc
    32221 agcagttcaa aatgcaaaag atttaattaa ccaaacaggt aatccaacac tcgacaaatc
    32281 acaagtagaa caattgacac aagcagtaac aactgcaaaa gataatctac atggtgatca
    32341 aaaacttgct cgtgatcaac aacaagcagt aacaactgta aatgcattgc caaacttaaa
    32401 tcatgcacaa caacaagcat taactgatgc tataaatgca gcgcctacaa gaacagaggt
    32461 tgcacaacat gttcaaactg ctactgaact tgatcacgcg atggaaacat tgaaaaataa
    32521 agttgatcaa gtgaatacag ataaggctca accaaattac actgaagcgt caactgataa
    32581 aaaagaagca gtagatcaag cgttacaagc tgcagaaagc attacagatc caactaatgg
    32641 ttcaaatgcg aataaagacg ctgtagacca agtattaact aagcttcaag aaaaagaaaa
    32701 tgagttaaat ggtaatgaga gagtcgctga agctaaaaca caagcgaaac aaactattga
    32761 ccaattaaca catttaaatg ctgatcaaat tgcaactgct aaacaaaaca ttgatcaagc
    32821 gacgaaactt caaccaattg ctgaattagt agatcaagca acgcaattga atcaatctat
    32881 ggatcaatta caacaagcag ttaatgaaca tgctaacgtt gagcaaactg tagattacac
    32941 acaagcagat tcagataaac aaaatgctta taaacaagct attgctgatg ctgaaaatgt
    33001 attgaaacaa aatgcgaata agcaacaagt ggatcaagca cttcaaaata ttttaaatgc
    33061 aaaacaagca ttaaatggtg atgaacgtgt agcacttgct aaaacaaatg gtaaacatga
    33121 catcgaccaa ttgaatgcat taaacaatgc tcaacaagat ggatttaaag gtcgcatcga
    33181 tcaatcaaac gatttaaatc aaatccaaca aattgtagat gaggctaagg cacttaatcg
    33241 tgcaatggat caattgtcac aagaaatcac tgacaatgaa ggacgcacga aaggtagcac
    33301 gaactatgtc aatgcagata cacaagtcaa acaagtatat gatgaaacgg ttgataaagc
    33361 gaaacaagca cttgataaat cgactggtca aaacttaact gcaaaacaag ttatcaaatt
    33421 aaatgatgca gtcactgcag ctaagaaagc attaaatggt gaagaaagac ttaataatcg
    33481 taaagctgaa gcattacaaa gattggatca attaacacat ctaaacaatg ctcaaagaca
    33541 attagcaatc caacaaatta ataatgctga aacgctaaat aaagcatctc gagcaattaa
    33601 tagagcaact aaattagata atgcaatggg tgcagtacaa caatatattg acgaacagca
    33661 ccttggtgtt atcagcagca caaattacat caatgcagat gacaatttga aagcaaatta
    33721 tgataatgca attgcgaatg cagcacatga gttagataaa gtgcaaggta atgcaattgc
    33781 aaaagctgaa gcagagcaat tgaaacaaaa tattatcgat gctcaaaatg cattaaatgg
    33841 agaccaaaac cttgcaaatg ccaaagataa agcaaatgcg tttgttaatt cgttaaatgg
    33901 attaaatcaa cagcaacaag atcttgcaca taaagcaatt aacaatgccg atactgtatc
    33961 agatgtaaca gatattgtta ataatcaaat tgacttaaat gatgcaatgg aaacattgaa
    34021 acatttagtt gacaatgaaa ttccaaatgc agagcaaact gtcaattacc aaaacgctga
    34081 cgataatgct aaaacaaact tcgatgatgc caaacgtcta gcaaatacat tgctaaatag
    34141 tgataacaca aatgtgaatg atatcaatgg cgcaatccaa gcagtcaatg atgcaatcca
    34201 taatcttaat ggtgatcaac gactacaaga tgctaaagac aaggcaattc aatctattaa
    34261 tcaagcttta gctaataagc taaaagaaat cgaagcttca aatgcgacgg atcaagacaa
    34321 gcttattgcg aaaaataaag cagaagaatt ggcaaacagc atcatcaaca acattaataa
    34381 agcaacaagt aatcaggctg tatctcaagt tcaaacagca ggcaaccacg cgattgaaca
    34441 agtgcatgcc aatgaaatac caaaagcaaa aattgatgcc aataaagacg ttgataagca
    34501 agttcaagca ttaattgacg aaattgatcg aaatccaaat ctaacagata aggaaaaaca
    34561 agcacttaaa gatcgtatta atcaaatact tcaacaaggt cataacggca ttaacaatgc
    34621 gatgactaaa gaagaaattg aacaagccaa agcacaactt gcgcaagcat tacaagacat
    34681 caaagattta gtgaaagcta aagaagatgc gaaacaagat gttgataaac aagttcaagc
    34741 tttaattgac gaaatcgatc aaaatccaaa tctaacagat aaggaaaaac aagcacttaa
    34801 atatcgaatt aatcaaatac ttcaacaagg tcataacgac attaacaatg cgctgactaa
    34861 agaagaaatt gagcaggcaa aagcacaact tgcacaagca ttgcaagaca tcaaagattt
    34921 agtgaaagct aaagaagatg cgaaaaatgc aataaaagcc ttagctaatg cgaagcgtga
    34981 tcaaatcaat tcaaatccag atttaacacc tgagcaaaaa gcaaaagcgc tcaaagaaat
    35041 tgacgaagct gaaaaacgag cactacaaaa cgttgagaat gctcaaacta tagatcaatt
    35101 aaatcgagga ttaaacttag gtttagatga cattagaaat acacatgtat gggaggttga
    35161 tgaacaacct gctgtaaatg aaatttttga agcaacacct gagcaaatcc tagttaatgg
    35221 tgaactcatt gtacatcgtg atgacatcat tacagaacaa gatattcttg cacacataaa
    35281 cttaattgat cagctttcag cagaagtcat cgatacacca tcaactgcaa cgatttctga
    35341 tagcttaaca gcaaaagttg aagttacatt gcttgatgga tcaaaagtga ttgttaatgt
    35401 tcctgtaaaa gttgtagaaa aagaattgtc agtagtcaaa caacaggcaa ttgaatcaat
    35461 cgaaaatgcg gcacaacaaa agattaatga aatcaataat agtgtgacat taacactgga
    35521 acaaaaagaa gctgcaattg cagaagttaa taagcttaaa caacaagcaa ttgatcatgt
    35581 taacaatgca cctgatgttc attcagttga agaaattcaa caacaagaac aagcgcatat
    35641 tgaacaattt aatccagaac aatttacgat tgaacaagca aaatcaaatg caattaaatc
    35701 gattgaagat gcaattcaac atatgattga tgaaatcaaa gctcgtactg atctaacaga
    35761 taaagagaag caagaagcta ttgctaagtt aaatcaatta aaagaacaag caattcaagc
    35821 gattcaacgt gcgcaaagca tcgatgaaat aagtgagcaa ttggaacaat ttaaagctca
    35881 aatgaaagca gctaatccaa cagcaaaaga actagctaaa cgcaagcaag aagctattag
    35941 tagaattaaa gacttttcaa atgaaaaaat aaatagtatt cgaaatagtg aaattggcac
    36001 agctgatgaa aaacaagcag caatgaatca aattaacgaa attgtgcttg aaacaattag
    36061 agatattaat aatgcgcata cattacagca agttgaggct gcattgaaca atggtattgc
    36121 tcgaatttca gcagtacaaa ttgtaacatc tgatcgtgct aaacaatcgt caagtactgg
    36181 aaatgaatct aatagccatt taacaattgg ttatggaact gcaaatcatc catttaacag
    36241 ttcgactatt ggacataaaa agaaacttga tgaagatgat gacattgatc cacttcatat
    36301 gcgtcacttt agtaataatt tcggtaatgt tattaaaaac gctattggtg tggtgggtat
    36361 ctctggttta ctagctagtt tctggttctt cattgccaaa cgtcgtcgta aagaagatga
    36421 agaggaagaa ttagaaataa gagataataa taaagattca ataaaagaga ctttagacga
    36481 tacaaaacat ttaccacttt tatttgcgaa acgtcgcaga aaagaagatg aagaagatgt
    36541 tactgttgaa gaaaaagatt cgctaaataa tggcgagtca ctcgataaag ttaaacatac
    36601 gccgttcttc ttaccaaaac gtcgtcgtaa agaagatgaa gaagatgtgg aagttacaaa
    36661 tgaaaacaca gatgaaaaag tgttgaaaga taacgaacat tcaccactct tattcgcaaa
    36721 acgacgcaaa gataaagagg aagatgttga aacaacaact agtattgaat ctaaagatga
    36781 ggacgttcct ttattattgg ctaaaaagaa aaatcaaaaa gataaccaat ccaaagacaa
    36841 aaagtcagca tcaaaaaata cttctaaaaa ggtagcagct aaaaagaaga aaaagaaagc
    36901 taagaaaaat aaaaaataat ttgtttcttt gataaataga ggagcaccga ttgacatcac
    36961 atcagtcggt gctcctttta tttattcttt ttaattaatt tatacaatgc ctgttgagcg
    37021 tgttgattcg cttctttgtt ttgttctctc ggtatccatt taacaaataa taaatcaaaa
    37081 tctttttcaa atatttctat ttgatcaaaa taaggtttga aatttgcgtt tttcacataa
    37141 ccagcttcaa tgctatctgc aattagcttt gagtctgtat ataatagtgc gttttgaaca
    37201 tttaattcac gtgcatgttc tagtgcataa atacatgcag cccattctgc agtgtggtta
    37261 tccatttcgc ctaactcatg tgtatatgta taatgctgct catcttcttt gattacaatg
    37321 gcacatgtac ttatgcctgg atttcctttc gtcgcagcat caaaatttat tttcgccata
    37381 ataaacctac tttctattca atacttagtt aaagttacta ttactgtaat acaaaatatg
    37441 ttgggtaatc cattaaaaaa cacgcatcac ttaaataagt aacacgtgtt taaaatactc
    37501 gctgattcaa agatgatttt ctaatacgta gtactgtaat atacttccta aaaaaatcat
    37561 cttcaggctg ggacataaat caatgttcta tgctctacga tgttatattg gcagtagttg
    37621 actgaacgaa aatgcgcttg taacaagctt ttttcaattc tagtcagggg ccccaacaca
    37681 gagaatttcg aaaagaaatt ctacaggcaa tgcgagttgg ggtgtgggtc ccaacacaga
    37741 agatgacgaa aagtcagctt acaataatgt gcaagtttgg gatgggcccc aacaaagaga
    37801 aattggattc ccaatttcta cagacaatgc aagttggggt gggacgacga aataaatttt
    37861 gcgaaaatat tatttctgtc ccactccctt aaaacttatt cttttgtgta gtaagtgcgt
    37921 taatagcctt gatctaactt atcaatctta cctttacgat aaaatgattt agcaatatat
    37981 ccaaatggta cattgaaaac tgttgaagct aattttaata cgtacgttgt aataaatatt
    38041 tcaaatacaa ctgtaccagg taaacttccg ataaatgcga tagctacaaa taaagctgta
    38101 tcaattattg agcttaaaaa tgtactacca tatgcacgga tgaaaaacgt tttatcagaa
    38161 ctaaatactt ttttaattag tgaaaagata aatacatcaa tatgttgacc aataatatat
    38221 gcgacgattg agcctaaagc aatgcgtggc acaacatcaa agattgcgtg taatgctttt
    38281 tgtgccatat cttctggtgc aggaataaaa tgtaatgaca attgcataac aataatcata
    38341 attaatgttg atgaaaagcc taaccaaact gctctttttg caactctacg cccataaata
    38401 tcgtttaata tatctgttgc taaataaata gaagcaaaca tgacattacc taaagttgct
    38461 gaaataccaa agatttctac agttttaatc acttgtatgt tggcaatgat tgtgccaatt
    38521 gcaacccatg caattaaacc ttgtttacca aaaaagcgat acataagtac cataagcacg
    38581 aacgttgcaa taaacgtaac tagtcctaaa atttcattat acatattaaa atgtcctcct
    38641 aaattttgat catgcgggtg tttagaaacc gctcaataaa taactaactc attttactga
    38701 aaaatcgttt gcgatgcaat agcatttgaa tataaataca ttttacgtat agaattgtaa
    38761 aaggtttcat tcaaatctta gggtcaaaaa tgttataata tttttatgtc aaatttaaaa
    38821 cagtaacact tatttacaag gttgcaatat tttgaagtaa taaaggaagt gtcgcgtatt
    38881 ttaacttttt cagagcaaaa tgcactcgcg aaaatagatg atttaatgaa tacttattgc
    38941 aatcaatgtc caatcaaaac tcgtctgcgt aaattagagg ggaaaacgaa ggcgcatcat
    39001 ttttgtatca atgagtgttc aatagggaaa gaaataaaac aattaggaaa tgaacttcaa
    39061 taggaggaag tcaaatgaaa attatatcta tatcagaaac accgaaccac aacacaatga
    39121 agattacact tagtgaaagc agagaaggta tgacatcaga tacgtatact aaagttgatg
    39181 attcacagcc agcatttatt aatgacatct taaaggttga aggcgttaaa tcaattttcc
    39241 atgttatgga ctttatttca gtagataaag aaaatgacgc aaattgggaa acagtattgc
    39301 caaaagtaga ggctgtattc gaataaattt ttcatcaact agtattcggg gggaataaag
    39361 tatatggaaa ttttacgtat agagccaaca ccaagtccaa atacaatgaa agttgttttg
    39421 tcatatacaa gagaagacaa gttatctaat acttataaaa aagtagaaga aacacaacca
    39481 agatttataa atcagttgtt atctatagat ggtatcactt ccatttttca tgtcatgaac
    39541 ttcttagctg ttgataaggc accaaaagct gattgggaag tcatattacc tgatattaaa
    39601 gctgcttttt ctgatgcgaa taaggtttta gaatctgtaa atgaacctca aattgacaat
    39661 cattttggtg aaattaaagc tgaattatta acttttaagg gtataccgta tcaaattaag
    39721 ctaacttctg ctgaccaaga attaagagaa caattaccac aaacatatgt tgaccatatg
    39781 actcaagcgc aaacagcaca tgacaatatt gtttttatgc gtaaatggct agatttagga
    39841 aatcgctatg gaaatattca agaagtaatg gatggtgtcc tagaagaagt gctaactacc
    39901 tatccagaat cacagttacc cgtattggta aaacatgctt tagaagaaaa tcacgcaact
    39961 aataattatc atttctatcg acatgtctct ttggatgaat atcatgcaac tgataattgg
    40021 aagactcgat tacgaatgtt aaaccatttt ccaaagccga cttttgaaga tataccgctg
    40081 cttgatttag ctttatctga tgaaaaagta ccggttagac gtcaagcgat tgtattatta
    40141 ggtatgattg aaagtaaaga aattttaccg tatttatata aggggcttcg tgataaaagt
    40201 cctgttgtaa gaagaacagc aggggattgc ataagcgatt tagggtatcc agaggcacta
    40261 ccagaaatgg tgctactatt agatgatcca cagaaaatcg ttaggtggcg tgctgctatg
    40321 tttatctttg atgaaggtaa tgcagagcag cttcccgcac taaaagccca tattaatgac
    40381 aatgcgtttg aagttaaatt acaaattgaa atggccatat ctcgtattga aaacggagat
    40441 gaagcacttg gttcagtatg gaaacaaatg gcaaacagaa caatttaatt ggaggaatta
    40501 aatatgaatg catatgatgc ttatatgaaa gaaattgcgc aacaaatgcg tggcgaatta
    40561 actcaaaatg gttttacaag tttagaaacg agcgaagcag tatcggagta tatgaaccaa
    40621 gtaaatgctg atgacactac ttttgtagtt attaactcta catgcggctg tgcagctgga
    40681 ttagcaagac cagctgcagt agcagttgca acacaaaatg aacatagacc tacaaataca
    40741 gttacagttt ttgctgggca agataaagaa gcaactgcta caatgcgaga attcattcag
    40801 caagcaccat ctagtccttc gtatgcttta ttcaaaggtc aagatttagt ttattttatg
    40861 cctagagaat ttatcgaagg tagagatatt aatgacattg caatggactt aaaggatgcc
    40921 tttgacgaaa attgtaaata gtacacataa ataaatataa aggttaacac attttataat
    40981 attaaaaatg gtgtctgtca ttgaaaatag agaatatagt tgtattctat ttgttaaata
    41041 aagtccgttt ttaccaacta tattttctag aaatttaact gttttaatag gacatcaaac
    41101 ataatattca aatcatgtgt taacctcttt tttaaaattt tttagcatta aagttataga
    41161 tttgggtaaa caattaccaa ttggaaacat atatcacgtt acgatggggt aggtacttaa
    41221 tcagcatttt ataaataaag taacggaatt catgatatta atatcatatt cctaaaatga
    41281 gtgataacaa aatgctacat aaagttaagt tatatcaaac taaatataca tactataaat
    41341 aatgaaaatg aggtgttatc gcatatgttg aattcatttg atgcagcata tcacagtctt
    41401 tgtgaagaag ttttagaaat aggaaataca cgaaatgatc gcacaaatac aggtacgatt
    41461 tcgaaatttg gtcatcaact tcgctttgac ttatctaaag gatttccact attaacgaca
    41521 aagaaagttt cttttaaatt agtagcaacc gaattattat ggttcattaa aggagataca
    41581 aacatccaat acttattaaa atataataat aatatatgga acgaatgggc ttttgaaaat
    41641 tatatcaaat cagacgagta taaaggtcca gatatgacag atttcgggca tcgtgcattg
    41701 agtgatcctg aatttaacga acaatataaa gaacaaatga aacaatttaa gcaacgtatt
    41761 cttgaagatg atacatttgc gaagcaattc ggggatttag gaaatgttta tggtaaacaa
    41821 tggcgagatt gggttgataa agatggtaat cattttgatc aacttaaaac agtaattgaa
    41881 caaattaagc ataatccaga ttcaaggcga cacatcgtat ctgcatggaa tccaacagaa
    41941 attgatacaa tggcacttcc gccttgtcat accatgttcc agttttatgt ccaagatggt
    42001 aagttaagtt gccagttata ccaacgtagc gcagatatct ttttaggtgt gccatttaat
    42061 atcgcaagct acgctttatt gacacacctt attgccaaag aatgtggact tgaagtgggt
    42121 gaatttgtgc atacatttgg agatgcacat atttattcaa atcatattga tgcgattcaa
    42181 acacaattag cacgtgaaag cttcaatcct ccaacattaa aaattaacag tgacaagtct
    42241 attttcgaca taaattatga agatttggaa attgttgact atgaatcaca tccagcaata
    42301 aaagctccaa tagcagtgta gtcattgcat agttagctaa ccatatagac atcaaaatga
    42361 catcatagta ttttcaagtg caaaaaagta cttttttgtg ttaaacgttt tcataaatta
    42421 tgcaaaatca ttatttctat cacactttat gataaaaatt gtgttaaatt aaagataact
    42481 tagtaataaa aaatgaaatg atagaagaag gaggataatt atgactttat ccattctagt
    42541 tgcacatgac ttgcaacgag taattggttt tgaaaatcaa ttaccttggc acctaccaaa
    42601 tgatttgaag catgttaaaa aattatcaac aggtcatact ttagtaatgg gtcgtaagac
    42661 atttgaatcg attggtaaac cactaccgaa tcgtcgaaat gttgtactta cttcagatac
    42721 aagtttcaac gtagagggcg ttgatgtaat tcactctatt gaagatattt accaactacc
    42781 gggccatgtt ttcatatttg gagggcaaac attatttgaa gaaatgattg ataaagtgga
    42841 cgacatgtat attactgtta ttgaaggtaa attccgtggt gatacgttct ttccacctta
    42901 tacatttgaa gactgggaag ttgcctcttc agttgaaggt aaactagatg agaaaaatac
    42961 aattccacat acctttctac atttaattcg taaaaaataa gggggaaaac gaccatgaca
    43021 aaacagatta tagtaacaga ctcaacatcc gatttatcta aagaatactt agaagcaaac
    43081 aacattcatg taattccttt aagtttaact attgaaggag cttcatacgt tgaccaagta
    43141 gatattacat cagaagaatt tattaatcat attgaaaatg atgaagatgt aaagacaagt
    43201 cagccagcca taggtgaatt tatatctgct tatgaagaac taggaaaaga tggctctgaa
    43261 atcataagta ttcatctttc ttcaggatta agtggtacat ataacactgc ttaccaagca
    43321 agtcaaatgg tagatgctaa tgtaactgtt attgattcaa aatctatttc ttttggttta
    43381 gggtatcaaa tacaacacct agtagagctt gtaaaagaag gtgtctcaac ttctgaaata
    43441 gttaaaaagt taaatcattt aagagaaaac attaaattat ttgtagttat agggcaattg
    43501 aatcaattaa ttaaaggtgg cagaattagt aaaacaaaag gtttgattgg taatcttatg
    43561 aaaattaaac caattggtac actagatgat ggtcgcttag agcttgtgca caatgcgaga
    43621 actcaaaatt ctagtatcca atacttgaaa aaggaaattg ctgaatttat aggagatcat
    43681 gaaatcaaat ccattggtgt cgcacatgct aacgtcattg aatatgttga taaattgaag
    43741 aaagttttta atgaagcttt tcatgtgaat aattacgata taaatgtaac tacaccagtt
    43801 atttctgcac atactggtca aggtgcgatt ggcctcgtag tccttaagaa gtaaatttaa
    43861 tcttttcagt gttaattact tccatttcaa tcctttatag actaaattta taattagata
    43921 gatagaggag gtaattcata tgacaaaaga atatgcaaca ttagcaggag gatgtttctg
    43981 gtgcatggtt aaaccattta catcatatcc aggcatcaag tcagtcgtat ctggttatag
    44041 tggcggtcat gttgacaacc caacttatga acaggtatgt acgaatcaaa ccggccatgt
    44101 cgaagcagta caaattacgt ttgatccaga ggttacttcc tttgaaaata tattagacat
    44161 atatttcaaa acatttgacc caactgatga tcaagggcaa tttttcgata gaggcgaaag
    44221 ctatcaacca gtcattttct atcatgatga acatcagaaa aaggctgctg agtttaaaaa
    44281 gcaacaatta aatgaacaag gtattttcaa gaaaccagtg attacaccta ttaaaccata
    44341 taaaaatttc tatccagctg aagactacca tcaagattat tacaaaaaga acccggtaca
    44401 ttattaccaa tatcaacgtg gttcaggtag aaaagcgttt atagaatcac attgggggaa
    44461 tcaaaatgct taaaaaagat aaaagtgaac taacagatat agaatatatt gttacacaag
    44521 aaaacggcac tgaaccacca tttatgaatg aatattggaa tcattttgct aaaggaattt
    44581 atgtagataa aatttctggt aaacctttat ttacatctga agaaaagttt cattctgaat
    44641 gtggatggcc tagcttttcc aaagcgcttg atgacgatga aattatagaa ttagtcgaca
    44701 aatcatttgg tatgttgaga actgaagtgc gttcagaaga atcaaatagt catttaggac
    44761 atgtctttaa tgatggacct aaagaaagtg gcggcttaag atactgtatc aattccgctg
    44821 caattcaatt tattccatat gaaaagttag aagaattggg ttatggcgat ttaatatcac
    44881 attttgataa gtaggagtgg aaagtatgtt taaaaaatta ttcggaaaag gcaaagaagt
    44941 tcagaaagat atcgcaattt atgcaccatt aactggagaa ttcgtgaaaa ttgaagatat
    45001 tccagatcct gtattcgcac aaaaaatgat gggcgaaggt tttggtatta atccaactga
    45061 aggagaagtt gtgtctccaa ttgcaggacg tgttgacaat gtctttccaa ctaagcatgc
    45121 gattgggtta aaagcagata acggattaga attattagtt catatcggtt tagacacagt
    45181 tcaattagac ggtgaaggat ttgaagtgtt agtgtctagt ggtgacgaag ttaacgtcgg
    45241 agatccatta gtaagattca accttgaata tattaataat aacgctaaat ctgtaatttc
    45301 accaattata attactaaca ctgatcaagc agcttcaatt aatatttatg atgaaaatgc
    45361 tgtgattaaa ggtgaaacaa aagtgattga tgtgacaatg aactaatgaa aaactattcg
    45421 ttttatcaat ttgtcatgac agttcgtggt cgacacgacg ataaaggtcg tctagcagaa
    45481 gagatatttg acgatcttgc tttcccaaaa cacgatgatg attttaacat actgtctgat
    45541 tatattgaga cacatggtga tttcacattg ccaatgtctg tatttgatga t
//
LOCUS       DAKTIO010000002        40653 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.002, whole genome shotgun sequence.
ACCESSION   DAKTIO010000002 DAKTIO010000000
VERSION     DAKTIO010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 40653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40653
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.002"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(408..506)
                     /locus_tag="O0293_000019"
     CDS             complement(408..506)
                     /locus_tag="O0293_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0278935.1"
                     /translation="MNWIIELDLWLWCVGAINLINAVNDYANEILL"
     gene            complement(711..1307)
                     /gene="recR"
                     /locus_tag="O0293_000020"
     CDS             complement(711..1307)
                     /gene="recR"
                     /locus_tag="O0293_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCY0278936.1"
                     /translation="MHYPEPISKLIDSFMKLPGIGPKTAQRLAFHTLDMKEDDVVQFA
                     KALVDVKRELTYCSVCGHITENDPCYICEDKQRDRSVICVVEDDKDVIAMEKMREYKG
                     LYHVLHGSISPMDGIGPEDINIPSLIERLKNDEVSELILAMNPNLEGESTAMYISRLV
                     KPIGIKVTRLAQGLSVGGDLEYADEVTLSKAIAGRTEM"
     gene            complement(1314..1631)
                     /locus_tag="O0293_000021"
     CDS             complement(1314..1631)
                     /locus_tag="O0293_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002479279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCY0278937.1"
                     /translation="MRGGGNMQQMMKQMQKMQKKMAQEQEKLKEERIVGTAGGGMVAV
                     TVTGHKEVVDVEIKEEAVDPDDIEMLQDLVLAATNEAMNKADELTQERLGKHTQGLNI
                     PGM"
     gene            complement(1721..3418)
                     /gene="dnaX"
                     /locus_tag="O0293_000022"
     CDS             complement(1721..3418)
                     /gene="dnaX"
                     /locus_tag="O0293_000022"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCY0278938.1"
                     /translation="MNYQALYRMYRPQSFEDVVGQEHVTKTLRNAISKEKQSHAYIFS
                     GPRGTGKTSIAKVFAKAINCLNSTDGEPCNECHICKGITQGTNSDVIEIDAASNNGVD
                     EIRNIRDKVKYAPSESKYKVYIIDEVHMLTTGAFNALLKTLEEPPAHAIFILATTEPH
                     KIPPTIISRAQRFDFKAISLDQIVERLKFVADAQQIECEDEALAFIAKASEGGMRDAL
                     SIMDQAIAFGDGTLTLQDALNVTGSVHDEALDHLFDDIVQGDVQASFKKYHQFITEGK
                     EVNRLINDMIYFVRDTIMNKTSEKDTEYRALMNLELDMLYQMIDLINDTLVSIRFSVN
                     QNVHFEVLLVKLAEQIKGQPQVIANVAEPAQIASSPNTDVLLQRMEQLEQELKTLKAQ
                     GVSVAPVQKSSKKPARGIQKSKNAFSMQQIAKVLDKANKADIKLLKDHWQEVIDHAKN
                     NDKKSLVSLLQNSEPVAASEDHVLVKFEEEIHCEIVNKDDEKRSSIESVVCNIVNKNV
                     KVVGVPSDQWQRVRTEYLQNRKNEGDDMPKQQAQQTDIAQKAKDLFGEETVHVIDEE"
     gene            complement(3487..4011)
                     /locus_tag="O0293_000023"
     CDS             complement(3487..4011)
                     /locus_tag="O0293_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCY0278939.1"
                     /translation="MQIYLSTLTELDYDKSLNSIEESFDDNPETSWQARAKVKHLRKS
                     PCYNFELEVIAKNENNDVVGHVLLIEVEINSDDKTYYGLAIASLSVHPELRGQKLGRG
                     LVQAVEERAKAQEYSTVVVDHCFDYFEKLGYQNAAEHDIKLESGDAPLLVKYLWDNLT
                     DAPHGIVKFPEHFY"
     gene            complement(4149..4250)
                     /locus_tag="O0293_000024"
     CDS             complement(4149..4250)
                     /locus_tag="O0293_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0278940.1"
                     /translation="MKTIQRIIRGTCLWEVAFLYVKFDSSELDVQFE"
     gene            complement(4319..4588)
                     /gene="ffs"
                     /locus_tag="O0293_000025"
     ncRNA           complement(4319..4588)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="O0293_000025"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(4650..5378)
                     /gene="treR"
                     /locus_tag="O0293_000026"
     CDS             complement(4650..5378)
                     /gene="treR"
                     /locus_tag="O0293_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HCY0278941.1"
                     /translation="MAKQKKFMKIYEALKEDILNGQIQYGEQIPSEHDLVQLYQSSRE
                     TVRKALDLLALDGMIQKIHGKGSLVIYQEVTEFPFSELVSFKEMQEEMGVAYLTEVVV
                     NEVVEAHEVPEVQHALNINSSESLIHIVRTRRLNQHVKIVDEDYFLKSIVSDIGNDVA
                     SDSIYDYLEKVLNLNISYSSKSITFEPFDEQAYQLFGDVSVAYSATVRSIVYLENTMP
                     FQYNISKHLANEFKFNDFSRRRIK"
     gene            complement(5403..7043)
                     /gene="treC"
                     /locus_tag="O0293_000027"
     CDS             complement(5403..7043)
                     /gene="treC"
                     /locus_tag="O0293_000027"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCY0278942.1"
                     /translation="MSKEIDWRKSVVYQIYPKSFNDTTGNGIGDINGIIEKLDYIKLL
                     GVDYIWLTPVYESPMNDNGYDISNYLEINEDFGTMDDFEKLIKVAHQKDLKVMLDIVI
                     NHTSTEHEWFKEARKSKDNPYRDYYFFRSSEDGPPTNWYSKFGGNAWKYDSETDEYYL
                     HLFDVSQADLNWDNPEVRQSLYRIVNHWIDFGVDGFRFDVINLISKGEFKDSDKIGKE
                     FYTDGPRVHEFLHELNRQTFGNTDMMTIGEMSSTTIENCIKYTQPERQELNSVFNFHH
                     LKVDYVDGEKWTNAKLDFHKLKEILMQWQRGIYDGGGWNAIFWCNHDQPRVVSRFGDD
                     TSEEMRIQSAKMLAIALHMLQGTPYIYQGEEIGMTDPHFTSIAQYRDVESINAYHQLL
                     SEGHAEADVLAILGQKSRDNSRTPMQWSDDVNAGFTAGKPWIDISENYHQVNVRQALQ
                     NKESIFYTYQKLIQLRHTHDIITYGDIVPRFMDHDHLFVYERHYKNQQWLVIANFSAS
                     AVDLPEGLAREGCVVIQTGTVENNTISGFGAIVIETNA"
     gene            complement(7107..8534)
                     /gene="treP"
                     /locus_tag="O0293_000028"
     CDS             complement(7107..8534)
                     /gene="treP"
                     /locus_tag="O0293_000028"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HCY0278943.1"
                     /translation="MAVKREDVKAIVTAIGGKENLEAATHCVTRLRLVLKDESKVDKD
                     ALSNNALVKGQFKADHQYQIVIGPGTVDEVYKQFIDETGAQEASKDEAKQAAAQKGNP
                     VQRLIKLLGDIFIPILPAIVTAGLLMGINNLLTMKGLFGPKALIEMYPQIADISNIIN
                     VIASTAFIFLPALIGWSSMRVFGGSPILGIVLGLILMHPQLVSQYDLAKGNIPTWNLF
                     GLEIKQLNYQGQVLPVLIAAYVLAKIEKGLNKVVHDSIKMLVVGPVALLVTGFLAFII
                     IGPVALLIGTGITSGVTFIFQHAGWLGGAIYGLLYAPLVITGLHHMFLAVDFQLMGSS
                     LGGTYLWPIVAISNICQGSAAFGAWFVYKRRKMVKEEGLALTSCISGMLGVTEPAMFG
                     VNLPLKYPFIAAISTSCVLGAIVGMNNVLGKVGVGGVPAFISIQKEFWPVYLIVTAIA
                     IVVPCILTIVMSHFSKQKAKEIVED"
     gene            complement(9005..9097)
                     /locus_tag="O0293_000029"
     tRNA            complement(9005..9097)
                     /locus_tag="O0293_000029"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9059..9061),aa:Ser,seq:tga)
     gene            complement(9366..10829)
                     /locus_tag="O0293_000030"
     CDS             complement(9366..10829)
                     /locus_tag="O0293_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HCY0278944.1"
                     /translation="MGEFKGFMKYDKQYLGELSLVDRLKHHKAYQQRFTKEDASIQGA
                     RCMDCGTPFCQTGQQYGRETIGCPIGNYIPEWNDLVYHQDFKTAYERLSETNNFPDFT
                     GRVCPAPCESACVMKINRESIAIKGIERTIIDEAFENGWVAPKVPSRRRDEKVAIVGS
                     GPAGLAAAEELNLLGYQVTIYERARESGGLLMCGIPNMKLDKDVVRRRIKLMEEAGIT
                     FINGVEVGVDIDKATLESEYDAIILCTGAQKGRDLPLEGRMGDGIHFAMDYLTEQTQL
                     LNGEIDDITITAKDKNVIIIGAGDTGADCVATALRENCKSIVQFNKYTKLPEAITFTE
                     NASWPLAMPVFKMDYAHQEYEAKFGKEPRAYGVQTMRYDVDDKGHIRGLYTQILEQGE
                     NGMVMKEGPERFWPADLVLLSIGFEGTEPTVPNAFNIKTDRNRIVADDTNYQTNNEKV
                     FAAGDARRGQSLVVWAIKEGRGVAKAVDQYLASKVCV"
     gene            complement(10847..15346)
                     /gene="gltB"
                     /locus_tag="O0293_000031"
     CDS             complement(10847..15346)
                     /gene="gltB"
                     /locus_tag="O0293_000031"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCY0278945.1"
                     /translation="MHNEKLIKGLYDYREEHDACGIGFYANMDNKRSHDIIDKSLEML
                     RRLDHRGGVGADGITGDGAGIMTEIPFAFFKQHVTDFDIPGEGEYAVGLFFSKERILG
                     SEHEVVFKKYFEGEGLSILGYRNVPVNKDAIAKHVADTMPVIQQVFIDIRDIEDVEKR
                     LFLARKQLEFYSTQCDLELYFTSLSRKTIVYKGWLRSDQIKKLYTDLSDDLYQSKLGL
                     VHSRFSTNTFPSWKRAHPNRMLMHNGEINTIKGNVNWMRARQHKLIETLFGEDQHKVF
                     QIVDEDGSDSAIVDNALEFLSLAMEPEKAAMLLIPEPWLYNEANDANVRAFYEFYSYL
                     MEPWDGPTMISFCNGDKLGALTDRNGLRPGRYTITKDNFIVFSSEVGVVDVPESNVAF
                     KGQLNPGKLLLVDFKQNKVIENNDLKGAIAGELPYKAWIDNHKVDFDFENIQYQDSQW
                     KDETLFKLQRQFAYTKEEIHKYIQELVEGKKDPIGAMGYDAPIAVLNERPESLFNYFK
                     QLFAQVTNPPIDAYREKIVTSELSYLGGEGNLLAPDETVLDRIQLKRPVLNESHLAAI
                     DQEHFKLTYLSTVYEGDLEDALEALGREAVNAVKQGAQILVLDDSGLVDSNGFAMPML
                     LAISHVHQLLIKADLRMSTSLVAKSGETREVHHVACLLAYGANAIVPYLAQRTVEQLT
                     LTEGLQGTVVDNVKTYTDVLSEGVIKVMAKMGISTVQSYQGAQIFEAIGLSHDVIDRY
                     FTGTQSKLSGISIDQIDAENKARQQSDDNYLASGSTFQWRQQGQHHAFNPESIFLLQH
                     ACKENDYAQFKAYSEAVNKNRTDHIRHLLEFKACTPIDIDQVEPVSDIVKRFNTGAMS
                     YGSISAEAHETLAQAMNQLGGKSNSGEGGEDAKRYEVQVDGSNKVSAIKQVASGRFGV
                     TSDYLQHAKEIQIKVAQGAKPGEGGQLPGTKVYPWIAKTRGSTPGIGLISPPPHHDIY
                     SIEDLAQLIHDLKNANKDADIAVKLVSKTGVGTIASGVAKAFADKIVISGYDGGTGAS
                     PKTSIQHAGVPWEIGLAETHQTLKLNDLRSRVKLETDGKLLTGKDVAYACALGAEEFG
                     FATAPLVVLGCIMMRVCHKDTCPVGVATQNKDLRALYRGKAHHVVNFMHFIAQELREI
                     LASLGLKRVEDLVGRTDLLQRSSTLKANSKAASIDVEKLLCPFDGPNTKEIQQNHNLE
                     HGFDLTNLYEVTKPYIAEGRRYTGSFTVNNEQRDVGVITGSEISKQYGEAGLPENTIN
                     VYTNGHAGQSLAAYAPKGLMIHHTGDANDYVGKGLSGGTVIVKAPFEERQNEIIAGNV
                     SFYGATSGKAFINGSAGERFCIRNSGVDVVVEGIGDHGLEYMTGGHVINLGDVGKNFG
                     QGMSGGIAYVIPSDVEAFVENNQLDTLSFTKIKHQEEKAFIKQMLEEHVSHTNSTRAI
                     HVLKHFDRIEDVVVKVIPKDYQLMMQKIHLHKSLHDNEDEAMLAAFYDDSKTIDAKHK
                     PAVVY"
     gene            15527..16411
                     /locus_tag="O0293_000032"
     CDS             15527..16411
                     /locus_tag="O0293_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0278946.1"
                     /translation="MEIKQLRYFIEVAKREHISETALELNIAQSAISRQITLLEQELN
                     VSLFKKQGRNITLTSEGKLLFNEALRIIEHLDSTIEQFQSHGLTKNKSIYIGYDESDV
                     SHMLLPLIQTFHLQNDTHVIPSLLEHDTIINSVLNGNIDIGFTELTPEIRKHKQLHML
                     PLFEEHYHLYAPSDDPITMATHPPLIQFEHSHIYCLAPFAETVKKQLRKITKSDVYTI
                     SSQPLAQYLLRQKEGYIISSQNIHLPESKSWIDIKLDHTELKRTICAITKEPYTKSDI
                     GILLTLIQQLMTKTSTFH"
     gene            16526..17644
                     /locus_tag="O0293_000033"
     CDS             16526..17644
                     /locus_tag="O0293_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCY0278947.1"
                     /translation="MFLKYRNLFTKPFNIILGLFCIAFIGAILFTFNNENFYNKPIGQ
                     IIDVKHVSSTPTKDAQNNRDIKYKNQLKVKILNGQFAGETTTINHQYVKSQADSEAFR
                     THEKVLLHISNKPSDAYIIEKKRDTLTVIITGLFLLTVLLVGRKVGLQSILSLILNSI
                     AILIAIYIHIQHSNINLFLLMTIAMICSTILTLLLVTGWHMRTLITIASTIIGTFLSI
                     GLTELIIYMADGKGIKYETMNFLSLPPKDIFLASVLIGSLGAIMDVAITIASGMHEIL
                     QRTPHISMRRWALAGRNIGQDIMGTMTNILLFSYLSGALPMFLIFLKNANTVTYTISM
                     NWSLEIARALTGGIGIVLTIPITILFMEIFETLRRAKQ"
     gene            17641..18423
                     /locus_tag="O0293_000034"
     CDS             17641..18423
                     /locus_tag="O0293_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCY0278948.1"
                     /translation="MNAVFILALLLLFLMIIFGGKKGFISYLTLFLNFAILIISIVLI
                     IFGVPIYVVTFFFCIIIGACNLFVLNSYNVKTQAAFISTIVTTILLITLIYLSVYFGH
                     LQGFSTEQQDETYVYSMNIGIDMVQFMVFTIILAVIAAVIDLTITISSPIYELHQVNP
                     TLTQYELFQSGMRVGREILATSANTIYLAFFGGQLALFIWFFKLKYSFGHIINSKIFA
                     QEFISIILGGIAVAISIPITAWITAFLIKRPSRQRKNDESIN"
     gene            complement(18592..19077)
                     /locus_tag="O0293_000035"
     CDS             complement(18592..19077)
                     /locus_tag="O0293_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0278949.1"
                     /translation="MTNNDTIMLRHYVPQDYSMLEAFQLSESDLKFVKTPEENITAAM
                     SDNERYPIVVMDGRQCVAFFTLHRGKGVAPFSDNQDAVFFRSFSVDQRYRNRGIGKVV
                     MEKLASFITSTFQDINEIVLTVNTDNPHAMALYRQQGYQYMGDSMFVGRPVHIMALTI
                     K"
     gene            complement(19067..19462)
                     /locus_tag="O0293_000036"
     CDS             complement(19067..19462)
                     /locus_tag="O0293_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCY0278950.1"
                     /translation="MIKCVCLVEETADKILLVQVRNREKYYFPGGKIEEGESQVHALL
                     REVKEELNLTLTMDEIEYVGTIVGPAYPQQDMLTELNGFRALTKIDWENVTINNEITD
                     IRWIDKDNDALIAPAVKVWIETYGGKHDK"
     gene            19611..19880
                     /locus_tag="O0293_000037"
     CDS             19611..19880
                     /locus_tag="O0293_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0278951.1"
                     /translation="MAKRSKSQRLSSLLNVAGFIVDGYNGYKYHAKNKKLVYLSLGLS
                     TVGTVLDFYISIKSPRKFKKAVAVVTLITNGARLFTSIRKVKHEY"
     gene            complement(20065..21069)
                     /gene="aaa"
                     /locus_tag="O0293_000038"
     CDS             complement(20065..21069)
                     /gene="aaa"
                     /locus_tag="O0293_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin/adhesin Aaa"
                     /protein_id="HCY0278952.1"
                     /translation="MQKKVIAAIIGTSAISAVAATQANAATTHTVKPGESVWAISNKY
                     GISIAKLKSLNNLTSNLIFPNQVLKVSGSSNSTSNSSRPSTNSGGGSYYTVQAGDSLS
                     LIASKYGTTYQNIMRLNGLNNFFIYPGQKLKVSGTASSSNAASNSSRPSTNSGGGSYY
                     TVQAGDSLSLIASKYGTTYQKIMSLNGLNNFFIYPGQKLKVTGNASTNSGSATTTNRG
                     YNTPVFSHQNLYTWGQCTYHVFNRRAEIGKGISTYWWNANNWDNAAAADGYTIDNRPT
                     VGSIAQTDVGYYGHVMFVERVNNDGSILVSEMNYSAAPGILTYRTVPAYQVNNYRYIH
                     "
     gene            complement(21401..22243)
                     /gene="gmpC"
                     /locus_tag="O0293_000039"
     CDS             complement(21401..22243)
                     /gene="gmpC"
                     /locus_tag="O0293_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter
                     glycylmethionine-binding lipoprotein"
                     /protein_id="HCY0278953.1"
                     /translation="MKRLIGLVIVALVLLAACGGNNDKKVTIGVASNDTKAWEKVKEL
                     AKKDDIDVEIKHFSDYNLPNKALNDGDIDMNAFQHFAFLDQYKKAHKGTKISALSTTV
                     LAPLGIYSDKIKDVKKVKDGAKVVIPNDVSNQARALKLLEAAGLIKLKKDFGLAGTVK
                     DITSNPKHLKITAVDAQQTARALSDVDIAVINNGVATKAGKDPKNDPIFLEKSNSDAV
                     KPYINIVAVNDKDLDNKTYAKIVELYHSKEAQKALQEDVKDGEKPVNLSKDEIKAIET
                     SLAK"
     gene            complement(22280..22939)
                     /locus_tag="O0293_000040"
     CDS             complement(22280..22939)
                     /locus_tag="O0293_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0278954.1"
                     /translation="MFGSDLDSAQLLQALYETLYMVSIALFLGAVIGIPLGVLLVITR
                     KQGIWPNIVIHQVLNPLINILRSLPFIILLIAIVPFTKLVVGTSIGTTAAIVPLTVYV
                     APYIARLVENSLLEVDEGIIEAAKAMGASPLQIIRYFLIPEALGSLVLAITTAIIGLI
                     GSTAMAGAVGGGGIGDLALVYGYQRFDTTVIIITVIVLVIIVQVIQTLGNVLARFIRR
                     H"
     gene            complement(22943..23968)
                     /locus_tag="O0293_000041"
     CDS             complement(22943..23968)
                     /locus_tag="O0293_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HCY0278955.1"
                     /translation="MIEFRQVSKTFNKKKQKIDALKDVSFTVNRNDIFGVIGYSGAGK
                     STLVRLVNHLEAASNGQVIVDGHDITNYSDKMMRDIKKDIGMIFQHFNLLNSATVFKN
                     VAMPLILSKKSKTEIKQRVTEMLEFVGLSDKKDQFPDELSGGQKQRVAIARALVTNPK
                     ILLCDEATSALDPATTASILTLLKNVNQTFGITIMMITHEMRVIKDICNRVAVMEKGK
                     VVETGTVKEVFSHPKTTIAQNFVSTVIQTEPSTSLIRRLNDEQVGDFKDYKIFVEETQ
                     VTQPIINDLIQICGREVKILFSSMSEIQGNTVCYMWLRFNMDQQFEDTAINQYFKEKN
                     IQFEEVH"
     gene            complement(24262..25404)
                     /locus_tag="O0293_000042"
     CDS             complement(24262..25404)
                     /locus_tag="O0293_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional cystathionine
                     gamma-lyase/homocysteine desulfhydrase"
                     /protein_id="HCY0278956.1"
                     /translation="MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYE
                     YSRTANPTRSSVESVIATLENGKHGFAFSSGVAAISAVVMLLDKGDHIILNSDVYGGT
                     YRALTKVFTRFGIEVDFVDTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEI
                     AKEHGLISVVDNTFMTPYYQNPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAE
                     RLAFISNSTGGILGPQDSYLLVRGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHP
                     SIESHLNHDVHMAQADGHTGVIAFEVKNTESAKQLIKATSYYTLAESLGAVESLISVP
                     ALMTHASIPADIRAKEGITDGLVRISVGIEDTEDLVDDLKQALDTL"
     gene            complement(25388..26302)
                     /locus_tag="O0293_000043"
     CDS             complement(25388..26302)
                     /locus_tag="O0293_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HCY0278957.1"
                     /translation="MITYDLIGNTPLVLLEHYSDDKVKIYAKLEQWNPGGSVKDRLGK
                     YLVEKAIQEGRVRAGQTIVEATAGNTGIGLAIAANRHHLKCKIFAPYGFSEEKINIMI
                     ALGAEVSRTSQSEGMHGAQLAARSYAEKYGAVYMNQFESEHNPDTYFHTLGPELTSAL
                     QQIDYFVAGIGSGGTFTGTARYLKQHHVQCYAVEPEGSVLNGGPAHAHDTEGIGSEKW
                     PIFLERRLVDGIFTIKDQDAFRNVKSLAINEGLLVGSSSGAALQGALNLKAQLSEGTI
                     VVVFPDGSDRYMSKQIFNYEENNNEQEN"
     gene            complement(26519..27856)
                     /locus_tag="O0293_000044"
     CDS             complement(26519..27856)
                     /locus_tag="O0293_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HCY0278958.1"
                     /translation="MKRQQSQWKSSTGFILASAGSAIGLGAMWKFPYMAGIYGGGAFL
                     AMFLIFTIFVGLPLLIMEFTVGKMGRTYTTQIYSKLTGKKWLNIIGWNGNLAVFILFG
                     FYSVIGGWIVIYIGQVLWQLVIFQRINHLQEMNFEAVISNPWLTVLGQGIFIFATMII
                     VMLGVEKGLEKASKVMMPLLFVFLIVIVIKSLTLDGALEGVKFILQPRVSEITADGIL
                     FALGQSFFTLSLGTTGMITYASYASKDMTIKSSAISIVVMNIFVSVLAGLAIFPALHS
                     FGYEPQEGPGLLFKVLPMVFSQMHLGTLFYLGFLVLFLFAALTSSISLLELNVSNFTK
                     NDNTKRKKVAVIGSILVFIISIPATLSFGILKDVRFGAGTIFDNMDFIVSNVLMPLGA
                     LGTTLVVGQLLDKKLLQQYFGKDRFRLFSGWYYLIKYAMPVVIILVFIVQLFS"
     gene            complement(28021..28134)
                     /locus_tag="O0293_000045"
     CDS             complement(28021..28134)
                     /locus_tag="O0293_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0278959.1"
                     /translation="MGIAPEVVMWRDVGDIDGVVCGEILAHVYSVEPENLM"
     gene            complement(28312..29046)
                     /locus_tag="O0293_000046"
     CDS             complement(28312..29046)
                     /locus_tag="O0293_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HCY0278960.1"
                     /translation="MRIKTPSPSYLKGTNGHAILLLHSFTGTNRDVKHLAAELNDQGF
                     SCYAPNYPGHGLLLKDFMTYNVDDWWEEVEKAYQFLVNEGYESISATGVSLGGLMTLK
                     LAQHYPLKRIAVMSAPKEKSDDGLIEHLVYYSQRMSNILNLDQQASSAQLAAIDDYEG
                     EITKFQHFIDDIMTNLNVIKMPANILFGGKDAPSYETSAHFIYEHLGSVDKELNGLKD
                     SHHLMTHGEGRDILEENVIRFFNALT"
     gene            complement(29083..29757)
                     /locus_tag="O0293_000047"
     CDS             complement(29083..29757)
                     /locus_tag="O0293_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCY0278961.1"
                     /translation="MIDKKLTSPKMTVPLFLIALIVFIGMFYSVVTNQEWLKNIDMGS
                     LTWFTDYFGEPQRQYVNNLFNYYMTFSAEIGDVKGVVLISIIVTIILFIKQRHLAVWF
                     VTYLVSGVIMNKLIKDTVLRPRPYNHLAVDTGFSFPSGHSNASTLLYFALMIIIISLA
                     AKTITKVLSALVMGILWLSILFCRLYFHAHYFSDVIGGTSLAIIWVALFLMVYPYFIN
                     HRRQRV"
     gene            29795..30211
                     /locus_tag="O0293_000048"
     CDS             29795..30211
                     /locus_tag="O0293_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0278962.1"
                     /translation="MLSIISMCHKFHFTDNIKIIKFILLCILQIKHCIIEITIFCYFQ
                     FSTIYIYYTEGVRRINRVIFVYIALIITLVRFFTPIHPSFSNLIYWIFVLYFIPIILC
                     VIGFKAEKLIATMVIIPNFLGILYRLYAYVTHILFM"
     gene            complement(30440..30802)
                     /locus_tag="O0293_000049"
     CDS             complement(30440..30802)
                     /locus_tag="O0293_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HCY0278963.1"
                     /translation="MKRTKGEIEAEISKAITQWEKDFLGRGSLSVKSDILRDMVIISL
                     QGILTPAEYRVCSTNEGLLNIKRTRSELVESGEQDLNDIIFKITGIKVMSFHSDLSTV
                     TGERIIVFKLEDNLEKHI"
     gene            complement(30964..33669)
                     /locus_tag="O0293_000050"
     CDS             complement(30964..33669)
                     /locus_tag="O0293_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcC family protein"
                     /protein_id="HCY0278964.1"
                     /translation="MTTQLNINSVIENAKRVITPLSPISIFAARNPWEGLEADTFEDV
                     AKWLRDVRDVDIFPNKALIESAVARGELDESVFNQLVTDMLLEHHYNIPQHYINLYID
                     NIKTLKDVPASYMNHSNVDVVADLLLEKSKRDMAESYHHYDVRPMSDAIIDEQGEPLS
                     EQVNRQMIKWTKLYIDQFLSSWTMPKREQSFYHAWLHLAQHDHSFTKAQRQVIKGLPN
                     DPEMTIESVLTHFSIDQEDYQAYVEGHLLALPGWAGMLYYRSQQHYFEQHLLTDYLAI
                     RLVVEQLLVGDEFKSVAKDCESRSENWFKQTVASWCYYSDMPSDVLLQHDVNEIQTFI
                     HFAATMNKNVFKNLWLIAWEMTYESQLKQKIKAGHESVAGALDVNQVNVSENDNANQP
                     HSVLLNDTQAVDENNSELNQMGTSTKAQIAFCIDVRSEPFRRHIEAAGPFETIGIAGF
                     FGLPIQKDAVDEQFKHDSLPVMVPPAYRIKEFADRYDMNVYRQQQQTMSSMFYTFKLM
                     KNNVMPSLLLPELSGPFLSLSTIVNSIMPRKSRASLQKIKQKWLKKPETKLTIDREFD
                     RTSDLPVGFTEQEQIDFALQALKLMDLTEAFAPFVVLAGHASHSHNNPHHASLECGAC
                     GGASSGFNAKLLAMICNRPNVRQGLKQSGVYIPETTVFAVAEHHTSTDTLAWVYVPDT
                     LSSIALDAYESLNDAMPMISEHANRERLDKLPTIGRVNHPVEEAQRFASDWSEVRPEW
                     GLAKNASFIIGRRQLTKGIDLEGRTFLHNYDWRKDKDGTLLNTIISGPALVAQWINLQ
                     YYASTVAPHFYGSGNKATQTVTSGVGVMQGNASDLMYGLSWQSVMAADRTMYHSPIRL
                     LVVIQAPDYVVARLLANNEHFARKVSNHWLRLMSVNEEGRFKSWI"
     gene            complement(33682..35166)
                     /locus_tag="O0293_000051"
     CDS             complement(33682..35166)
                     /locus_tag="O0293_000051"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit 5"
                     /protein_id="HCY0278965.1"
                     /translation="MLSFQLLFSLFVIALIIALISGLLFLAPVMPMRYIKLHLYILVM
                     PVLFAVIGFFGIHGQHVLGPFKIDRLSWLLAGFVMALGFIIQKFSMRYLLGDHHYRHY
                     FPLFTAITSFASLAWMSEDLRLMALCWGITLLCLTLLMNVNRFWKVPRESAKLSSMTF
                     LCGWLAFVGAIVTIYIATGEWRVPQHIVHPTWSLLTNVLLVLAVMIPAAQFPFHRWLI
                     ESVTAPTPVSAIMHAGIVNAGGVILTRFAPIFDNGFALSLLLILSSISVLLGSGISLV
                     QVDYKRQLVGSTMSQMGFMLVQCALGVYSAAIIHLILHGIFKATLFLQSGSIVKRFNI
                     PKQASAKDAYGWIVMGRVLAIIVAFLFWMSSDRSAYEVLSALILGWSLLVSWNQMVAF
                     SKGRMARLVGMILIAIVTFIYVITHNYFYAVLQNITTHATTPPTVSVIISVVILIFGS
                     LLSIWVARHRYSKAFAVLYVWLVNLGEARSKAIESHPNYLKKYL"
     gene            35687..35752
                     /locus_tag="O0293_000052"
     CDS             35687..35752
                     /locus_tag="O0293_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-1"
                     /protein_id="HCY0278966.1"
                     /translation="MGIIAGIIKVIKSLIEQFTGK"
     gene            35784..35849
                     /locus_tag="O0293_000053"
     CDS             35784..35849
                     /locus_tag="O0293_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-2"
                     /protein_id="HCY0278967.1"
                     /translation="MGIIAGIIKFIKGLIEKFTGK"
     gene            35901..35969
                     /locus_tag="O0293_000054"
     CDS             35901..35969
                     /locus_tag="O0293_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-3"
                     /protein_id="HCY0278968.1"
                     /translation="MEFVAKLFKFFKDLLGKFLGNN"
     gene            36033..36095
                     /locus_tag="O0293_000055"
     CDS             36033..36095
                     /locus_tag="O0293_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-4"
                     /protein_id="HCY0278969.1"
                     /translation="MAIVGTIIKIIKAIIDIFAK"
     gene            complement(36754..37956)
                     /locus_tag="O0293_000056"
     CDS             complement(36754..37956)
                     /locus_tag="O0293_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCY0278970.1"
                     /translation="MAKIPVTVLSGYLGSGKTTLLNHILQNREGRRIAVIVNDMSEVN
                     IDKDLVADGGGLSRTDEKLVELSNGCICCTLRDDLLKEVERLVKKGGIDQIVIESTGI
                     SEPVPVAQTFSYIDDELGIDLTAICRLDTMVTVVDANRFVHDINSEDLLMDRDQSVDE
                     TDERSIADLLIDQVEFCDVLIINKIDLISEEELAKLEKMLSALQPTAKIIKTTNSEVD
                     LKEVLNTQRFDFEKASESAGWIKELESGGHASHTPETEEYGISSFVYKRRLPFHAKRF
                     NDWLESMPNNVVRSKGIVWLAQYNHVACLLSQAGSSCNIHPVTYWVASMSEAQQTQIL
                     AERQDVAAEWDPEYGDRHTQFVIIGTELDEEKLTKELDACLVNAQEIDADWQQFEDPY
                     QWQIRPAR"
     gene            38075..38335
                     /locus_tag="O0293_000057"
     CDS             38075..38335
                     /locus_tag="O0293_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0278971.1"
                     /translation="MSALLYNGDKDISHERESRCINMLFVILVLYVTGIAFILLSVFG
                     SKTEGLSTKHTLYTIGSAIITIAIFISIGYAIQYLTAALYGL"
     gene            complement(38625..38939)
                     /locus_tag="O0293_000058"
     CDS             complement(38625..38939)
                     /locus_tag="O0293_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0278972.1"
                     /translation="MTLIEPDMTLRMPDISTTVETLNLISKMEAQKENIRTVIAPEHK
                     HKYKDIENGLKGEEKVLIEQMAQHCEAFKANFKGAAQGDWVKSAMSEIDSIKDDLKKI
                     NS"
     gene            complement(38958..39281)
                     /locus_tag="O0293_000059"
     CDS             complement(38958..39281)
                     /locus_tag="O0293_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0278973.1"
                     /translation="MMFNQINNKNELEESYESEKKRIENELQNLNELRHRTRKENERS
                     YDVFQYLKHEMNYSEDAQRKMTRNIEAYEQEINEIIRKQEWKLEEYKEDLKKSYEKQL
                     DKLSD"
     gene            complement(39278..40606)
                     /locus_tag="O0293_000060"
     CDS             complement(39278..40606)
                     /locus_tag="O0293_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0278974.1"
                     /translation="MLSRKYKIDLKAINQNTESTSAISKASYEVENANNNGLSKRDVI
                     NQFNDLKKMKKFPSNLEYVDSYTDSLTGVTTSAFLNKDTGKVTLGMTGTNLQDEAFKK
                     LKEGEFSRQNVTNALETVKDGYADLKILYSPASDQNYRYANTQEFINKIKSKYDIDFI
                     TGHSLGGRDAVVLGMSNGIPNIVVYNPAPISITSLNPNSPDGKRLLELYKNYKGNITR
                     FVAENDALTENLKKYKHYVFFGNDKVFKNGKGHEMEGFLTEEEQKAIKKELKKLQGYA
                     EENNKSFVKNSNNAISKLASIELLRANMMTTNGGGLSSSQQKVLESLTALTIAQSFSQ
                     LIDDEINQIKKMYNEKKKKFGKNWEDAQKAGKAVGEDLSVNGVLNALDEGQVNESSMV
                     REPEQMISAKERQLSTIGSSVSNYIMRVRLSINEIVDKDQVLASQIGGLL"
BASE COUNT        12747 a         8367 c         5893 g        13646 t
ORIGIN      
        1 aacatttaag tcaataactt tttaaaatta attttcgtta gtaattcaag ttatttctta
       61 agttgagttg ttttgtttgc ctaaacaaat aaatactata caggctttca aactgcttaa
      121 caacagtaaa aattacacct ttgtcatctg tactgaacgc ccttatccta aaaatcgaac
      181 ttaaaaaatc cccacttata aatagtacgt tttttagaac aagaaacaga tttttctcta
      241 accatatcaa tcaaaaaaaa cgcaaaacag aacgttattt ttttaccaga aattaaaaat
      301 aaatttacaa gttttaaata atctctcaaa acaccgctat acaggctgat ttaataagct
      361 ttagaccaaa gaagtacaac ttactaagcc agcatctatc atttcaatta caaaagaatt
      421 tcatttgcat agtcattaac cgcatttatc aaattaatag caccaacaca ccataaccat
      481 agatctaatt cgataatcca attcacatca ttactattag aagtaacaaa tcacaaccaa
      541 cggcttgctt cctaaagatt acccatccct cagtaaaact aggtaaacca taccaccgcc
      601 acaccgcaaa taaagtagta catcacacca caccataaaa aaagccacaa tgattacact
      661 gtgacttttc ttactatagt attaaaatca aaaatgttta atagaagaca ttacatttct
      721 gttctacctg cgattgcttt agataatgtt acttcgtcag catactctaa atcgccacct
      781 accgataacc cttgtgctaa tctcgtcact ttgataccta taggcttaac taatctagaa
      841 atatacatgg ctgtagattc cccctctaag ttcgggttca tagctaagat taattcgcta
      901 acttcatcgt ttttcaagcg ttcaatcaat gaaggaatat taatatcttc tggtccaatg
      961 ccatccatag gcgaaataga cccatgtaaa acgtgatata aacctttgta ttctctcatt
     1021 ttttccatag ctatgacatc tttgtcatct tccacaacac aaataactga acgatctctt
     1081 tgcttatctt cacaaatata acatggatca ttttcagtaa tgtgaccaca tacgctacaa
     1141 tatgttaatt ctctcttaac atctactaat gctttggcaa actgaacaac atcgtcttct
     1201 ttcatatcta aggtatgaaa agccagacgt tgggctgtct ttggaccaat gcctggcaat
     1261 ttcataaagc tatcaataag ttttgatata ggttctggat aatgcatcta tgatcacatt
     1321 ccagggatgt ttaagccttg agtatgttta cctaaacgtt cttgagtaag ctcatcagct
     1381 ttattcatcg cttcattagt agctgctaac actaagtctt gtagcatttc aatatcgtct
     1441 gggtctacag cttcttcttt gatttcaacg tcgacaactt ctttatgacc agttacagta
     1501 actgcaacca tgccaccgcc agctgttcct acaatacgct cttctttaag tttttcttgt
     1561 tcttgagcca ttttcttttg cattttttgc atttgtttca tcatttgttg catgtttccg
     1621 ccaccgcgca tattccattt cctccttgaa aatcaataaa tatttatcaa taaaatgatg
     1681 tttctttcat tatacatacg attatatcgc ttgtcatgta tcactcttca tctatcacat
     1741 gtacagtttc ttcaccgaaa agatcttttg ctttttgagc aatatctgtt tgttgtgctt
     1801 gttgctttgg catatcatcg ccttcgtttt tacgattttg taaatattcc gttcgaactc
     1861 tttgccattg atctgatggt acaccaacaa ctttaacgtt tttattaacg atattacata
     1921 caacactttc tatactacta cgtttctcgt cgtctttatt gacgatttca caatggatct
     1981 cttcctcaaa tttcacaagt acgtgatctt cacttgccgc cacaggttcc gaattttgca
     2041 ataaactaac gagtgatttt ttatcattat ttttggcatg atcaatcact tcttgccaat
     2101 gatctttcaa caatttgata tctgccttat tcgctttatc tagcactttt gcaatttgtt
     2161 gcattgaaaa tgcattttta gatttttgta tgcctctcgc aggctttttc gaagattttt
     2221 gaacaggagc gacactcact ccttgtgctt ttagtgtttt tagttcttgc tctaactgtt
     2281 ccatacgttg caacaataca tctgtgtttg gcgatgaagc aatttgtgct ggttcagcta
     2341 cattcgcaat cacttgtggt tgacccttaa tctgctcagc taattttact aacaacactt
     2401 caaaatgaac gttttgattc acactaaaac gaatcgacac taatgtatca ttaataagat
     2461 caatcatttg atataacata tctaattcta agttcatcag tgctcgatac tcagtatctt
     2521 tctcagatgt tttattcata atcgtatctc tgacaaaata aatcatatca tttattaggc
     2581 gattcacttc tttaccttct gttataaact gatggtattt tttaaaagat gcttgtacgt
     2641 caccttgtac aatatcatca aacaagtgat ccaacgcttc atcatgtacg ctacctgtga
     2701 cattcaacgc atcttgcaat gttaacgtac catcaccaaa tgcaatagcc tgatccataa
     2761 tacttaatgc atcacgcata cccccttcag acgctttagc gataaatgcc aaggcttcat
     2821 cttcacattc aatttgttgt gcatctgcta caaattttaa acgttcaaca atttgatcta
     2881 ggctaattgc tttaaaatca aaacgttgtg ccctagaaat gattgttgga gggattttat
     2941 gtggttctgt cgttgccaat ataaaaatag cgtgtgctgg aggttcttct aacgtcttta
     3001 aaagggcatt aaaagcacct gttgttagca tgtgcacctc atctataata taaactttat
     3061 atttcgattc acttggtgca tatttaactt tgtctctaat atttcttatt tcatcaaccc
     3121 cattattact agcagcatca atttctatca catctgaatt agtcccctgc gtaatgcctt
     3181 tacaaatatg acattcatta caaggttctc catcagtgct atttagacag ttgattgctt
     3241 tagcaaacac tttggcaata ctcgttttcc ccgtacctct cggaccacta aaaatataag
     3301 catgcgactg tttttctttc gaaatcgcat tgcgcaatgt cttcgtgaca tgttcttgtc
     3361 cgacgacatc ctcgaaactt tggggtctgt acatacgata taaggcttga taattcaagt
     3421 tagcacctcc ataaacaatt acctctcatt atagcatgat aataccttta cttcttaatt
     3481 gaacaattaa taaaaatgtt ctggaaattt tacgattccg tgtggtgcat ccgtcaaatt
     3541 atcccataaa tattttacaa gtaacggtgc atcaccagat tctaatttaa tgtcatgctc
     3601 agcagcattt tgataaccca acttttcaaa gtagtcaaaa caatggtcta caacaaccgt
     3661 actatactct tgtgctttgg cacgctcttc tactgcttga accaagccac gacctaattt
     3721 ttgtccacgt aattcaggat gaactgataa agaggcaatc gccaaaccat aatacgtctt
     3781 atcatcacta ttaatttcta cttcaattaa taaaacgtgt ccaacgacat cgttattttc
     3841 atttttcgct attacttcta attcaaaatt atagcaagga gattttctta aatgttttac
     3901 tttcgcacgt gcttgccaac tcgtttcagg attatcatca aaactttctt caatactatt
     3961 taaagattta tcataatcta actctgttaa agtacttaaa tatatttgca tatgtcctcc
     4021 gtaggcattt gattgtcaat aatcataacg tatctcatta ataattctat tgtaagatac
     4081 tccccctcat ttcaccatca tttctttatc atcaaattat aacttcttac ttttcattga
     4141 cacaaaaatc attcaaactg cacatcgagt tcacttgaat caaacttcac atataaaaaa
     4201 gctacttccc acaaacatgt tccacgtata atacgctgaa ttgtcttcaa gaaagtagct
     4261 tctataatta tatattttca actcttaata atcggtttaa tatttaaaaa ataaaaaacc
     4321 gtgcacctaa gcatcgaacg taattcacga acgtaaccaa agtcgttagc taaatctcgg
     4381 ctaccctacg gcacatatga tgatccactt aatgctgctt ccgtcaggac ctgacatggt
     4441 tcatgggttc atattgcata ggaccgaaat cttcaaacac tacgtgcttt gggcagactt
     4501 cgcaaaaata cggcctcaac aaaggaatta agcctcgcat aaagcggatt tcgagtacag
     4561 ggaaccgcta cctccccacc tagcacggca agatatatat tactatattt taatagttaa
     4621 ttgcaagtat aaatcattta tatcattgtt tactttatac gacgtcttga gaagtcatta
     4681 aatttaaatt catttgcaag atgttttgaa atattatatt gaaacggcat tgtattttct
     4741 aaatacacaa tacttcgaac tgttgctgaa taagccaccg atacatcacc aaacaattga
     4801 tatgcttgtt catcaaacgg ttcaaaagta atagacttac ttgaataact aatattaaga
     4861 tttaatacct tttccaaata atcataaata gaatcactcg caacatcatt acctatatct
     4921 gaaacaatcg actttagaaa ataatcttca tcaacaatct tcacatgttg gttaagccga
     4981 cgagttctaa caatatgaat gagtgattca ctagaattga tgtttaaagc atgttgaact
     5041 tctggaactt catgcgcttc aacaacctca ttcacaacaa cttcagttaa atatgcgacg
     5101 cccatttctt cttgcatttc tttaaaacta acaagttcag aaaatggaaa ctctgtaacc
     5161 tcctgataaa tgacaagtga ccctttacca tgaatctttt gaatcatgcc gtctaatgcc
     5221 aacaaatcta atgccttacg cacggtctct cgagatgact ggtacaattg caccaaatca
     5281 tgttcagacg gaatttgttc accatattga atctgcccgt ttaatatatc ttctttcaac
     5341 gcctcataaa tcttcataaa ctttttttgt ttcgccatat aaacgcatcc actcaattta
     5401 ttttacgcgt ttgtttcgat tacaattgca ccaaacccgc ttatcgtatt attttccact
     5461 gtgcctgttt gaatcacaac acaaccttct ctagccaatc cttctggcaa atcaacagcc
     5521 gatgctgaga aattcgcaat tactagccat tgttgattct tataatgacg ttcataaaca
     5581 aataaatgat catgatccat aaaacgtggc acaatgtctc catacgtaat aatatcatgc
     5641 gtatgtctta attgtattaa tttttgatac gtatagaaaa tagactcttt attctgaagt
     5701 gcttgtctaa cgttgacctg atgataattt tccgaaatat caatccaagg cttaccagct
     5761 gtaaatccag cattaacatc atcactccat tgcataggcg ttctcgaatt gtctcgtgac
     5821 ttctgtccta aaatcgctaa cacatccgct tcagcatgcc cttcacttaa caactgatgg
     5881 taggcattaa tcgattcaac atcacgatat tgtgctattg atgtaaaatg tgggtccgtc
     5941 ataccaattt cttcaccttg gtaaatatat ggcgtccctt gcaacatatg cagtgcgata
     6001 gctaacattt tagcactttg tatcctcatc tcttccgacg tatcatcacc aaatctagac
     6061 actacccgtg gctgatcatg attacaccag aaaatcgcgt tccatccgcc accgtcataa
     6121 atacctcgtt gccattgcat cagaatttcc tttaacttat gaaaatcaag cttcgcattt
     6181 gtccactttt caccatcaac ataatcaacc tttagatgat gaaaattaaa aacactattc
     6241 aattcttggc gttctggttg tgtatactta atacaatttt caatcgtcgt cgaagacatt
     6301 tctcctatag tcatcatgtc agtgttacca aacgtttgac gatttaattc atgcagaaac
     6361 tcatgcactc taggaccatc cgtataaaat tctttaccta ttttgtcaga gtccttaaat
     6421 tcacctttag aaattaagtt aatgacatca aatcgaaaac catcaacgcc gaagtctatc
     6481 caatgattga ctatgcgata taacgattga cgtacttccg gattatccca atttaaatca
     6541 gcttgactga catcaaataa atgtaaataa tattcatctg tctcagaatc atacttccat
     6601 gcattaccac cgaatttaga ataccaattt gttggcggcc cgtcttcaga tgatctgaaa
     6661 aagtaataat ctctataagg gttatcttta gatttacggg cttctttaaa ccattcatgc
     6721 tccgtcgacg tatgattaat gacaatatct aacatcactt tcaagtcttt ttgatgcgca
     6781 actttgatta acttttcaaa atcatccatc gttccaaagt cttcattgat ttctaaataa
     6841 ttgctgatat catagccatt atcattcatc ggtgattcat acactggtgt taaccaaata
     6901 taatcaacac ccaataactt gatataatcc aatttttcta taattccatt gatatctcct
     6961 ataccattcc ccgtcgtatc attaaacgac ttaggataaa tttgatatac aacggatttt
     7021 ctccaatcta tttctttcga cacacaatta ccaccttata acgtaataaa ggacgtccaa
     7081 ataacgaacg cccctttttt attttattaa tcttcaacaa tttctttcgc tttttgttta
     7141 ctaaaatgag acatcacaat tgttagtata catggtacaa caatagcaat agctgtcaca
     7201 ataagatata ctggccaaaa ttctttttga attgaaatga atgctggcac gccaccaaca
     7261 ccaacttttc caagtacgtt attcatacca acgattgccc ccaatacaca agacgttgat
     7321 atcgcagcga taaatggata tttcagaggt aagttcacac cgaacatggc tggttcagta
     7381 acacctaaca taccagaaat acaagatgtt aatgccaagc cttcttcttt aaccatttta
     7441 cgacgtttat agacaaacca tgctccaaat gctgcagagc cctgacaaat attggaaatc
     7501 gcaacaattg gccataaata cgtaccgcct aagctgctac ccatcaattg gaaatctact
     7561 gctaaaaaca tatggtgtag tcctgtaatt acaagtggtg catataacaa tccatatatt
     7621 gctccgccaa gccatcctgc atgttggaat ataaatgtaa caccagatgt aatacctgta
     7681 ccaatcaata acgcaactgg tccaatgata ataaatgcta aaaatccagt aactaaaagc
     7741 gctacgggtc caacgaccaa catttttatc gaatcgtgaa cgactttatt taatcctttt
     7801 tcaattttag ctagaacgta agctgcaatt aaaactggca acacttgacc ttggtaattc
     7861 aactgcttaa tctctaagcc aaataagttc cacgtcggaa tattcccttt tgccaaatca
     7921 tactgagata ctaattgcgg atgcattaaa atcaaaccta agactatgcc tagaatcgga
     7981 ctaccaccaa atacacgcat actactccaa ccaattaatg ctggtaagaa aataaatgcc
     8041 gtactcgcaa tcacattaat gatgtttgaa atatcagcaa tttgtggata catctcaata
     8101 agtgcttttg gaccaaataa acctttcatt gtaagtaaat tattgattcc cattaacaaa
     8161 ccagctgtca caatcgcagg taatattggt ataaaaatat cccccaacaa tttgatcaaa
     8221 cgttgtactg gattcccttt ttgtgcagct gcttgtttcg cttcatcttt cgaagcttct
     8281 tgagcacctg tttcatcaat aaactgctta tacacttcat cgactgttcc tggaccaatg
     8341 acaatttgat attgatggtc tgctttaaac tgccccttga ccaacgcgtt attacttaat
     8401 gcgtctttat caactttact ttcatccttc agcactaaac gtaatcgtgt tacacaatgc
     8461 gttgcagctt caagattttc ttttccccca atagcggtta cgatggcttt tacatcttct
     8521 ctttttacag ccatatattt cactccctta atgtcttaaa acgagtataa cttgtctaga
     8581 caagttatgt aaaggctttc aacaaaatat ttttattttg tcacaattat ctggggtagc
     8641 catttcagaa aatcaatgag ccataacgac atatgtagca ataactcctc gctacaattg
     8701 cgttattaac aatacctcac cacgctctca ccattataaa gtgtagatgc atggccaact
     8761 cctaaattga aaaatgcata tctataaata gcaaacttgt ccacacaagc catccatcat
     8821 atttcacaaa acaaaaaaga catttagcta tgtatattgc acaagccaaa tgcccttcca
     8881 atctcaatgc cttatcattt ctattatcaa aaattacgag ccattcaaag acaaataaaa
     8941 aaggtaaata ctcaaaattt gagcacttac cttcattata tatagaattt aatataaata
     9001 ttgatggcgg aggaagaggg attcgaaccc ccgcgagccg ttaagcccct gtcggttttc
     9061 aagaccgatc ccttcagccg gacttgggta ttcctcctaa acacaatatt tatcatatat
     9121 gaacgttctg taaaagtcaa tattgttttt aagcattaca tgattaccac aaatcattgt
     9181 cttccactac agttgaccta taaacgaatg taaaaaatca ctacttataa ttaatctatt
     9241 tgttgtctta caaacctttg tttttaggtt acaaagatgg catgtgacgt tgatagatac
     9301 tagatttttt caaactcgat tcagcatgtc acaacgtcaa cgtaaccacc atttccatac
     9361 aaagattata cacaaacttt actagctaaa tactgatcta ctgctttcgc tacgcctcta
     9421 ccttctttaa ttgcccatac aactaaactt tgaccacgtc tagcatctcc agcagcaaat
     9481 accttttcat tattagtttg atagtttgta tcatccgcca cgattcgatt tctatccgtt
     9541 ttaatgttaa aagcattcgg tactgttggt tctgtacctt cgaagccgat tgataataat
     9601 acaaggtcag caggccaaaa tctttcaggt ccttctttca tgaccatacc attttcgcct
     9661 tgctctaaaa tttgagtata caaaccacgt atgtgtcctt tatcgtcaac atcgtaacgc
     9721 attgtttgaa caccatatgc acgtggttcc ttaccaaact tagcttcgta ctcttggtgc
     9781 gcatagtcca ttttaaacac cggcattgct aaaggccatg atgcattttc tgtaaatgta
     9841 attgcttctg gcaatttcgt atatttatta aattgaacaa tcgatttaca attttctctt
     9901 aatgctgtcg ctacacagtc tgcccctgta tcaccagcac caatgataat gacattctta
     9961 tcttttgcag ttattgttat atcatcaatt tctccattta acaactgcgt ttgttcagtt
    10021 aaataatcca tagcgaaatg tataccatca cccatgcgtc cttctaaagg taaatctcta
    10081 cctttttgtg caccagtaca taatataatg gcatcatact cagattctaa cgttgcttta
    10141 tcaatatcaa cgccgacttc aacaccatta atgaaagtaa tgcccgcttc ttccattaac
    10201 ttaatacgac gtcgaaccac atctttatca agtttcatat tcggaatacc acacattaat
    10261 aaaccgcctg attctctagc acgttcataa atagttactt gatatcctag tagattaagt
    10321 tcttcagcag cagctaatcc tgctggaccg cttccaacga ttgccacttt ttcatctcta
    10381 cggcgactcg gaactttcgg cgctacccaa ccattttcaa aagcttcatc aataattgtg
    10441 cgttcaatac ctttaatcgc aatcgattct ctattaatct tcatcacaca agcactttcg
    10501 catggtgcag gacatacacg ccctgtaaag tcaggaaagt tatttgtttc gcttaagcgt
    10561 tcataagcag ttttaaaatc ttgatgatac actaagtcgt tccattcagg aatgtagttt
    10621 ccaattggac aacctattgt ttccctacca tactgttgtc cggtttgaca aaacggcgtt
    10681 ccacaatcca tacatcgtgc accttggata gaggcatctt ctttagtaaa tcgttgttga
    10741 tatgctttat gatgcttcaa acggtctacc agtgataatt cacctaagta ctgtttgtca
    10801 tacttcataa atcctttaaa ttcacccatc gtatctcccc ctttccttaa tacacaacgg
    10861 ctggtttatg tttagcatcg attgttttac tgtcatcgta aaatgcagct aacatcgctt
    10921 catcttcatt gtcatgtaat gatttgtgca aatgaatttt ttgcatcatt aattgataat
    10981 ctttaggaat aactttaacg acgacatctt caatgcgatc aaaatgtttt aacacatgaa
    11041 tcgctctcgt actattcgtg tgtgacacat gttcttccag catttgctta atgaatgctt
    11101 tttcttcttg gtgtttaatc tttgtaaacg aaagcgtatc tagttgatta ttttcaacaa
    11161 aagcttctac atcagacggg ataacgtaag caataccacc actcatacct tgaccgaagt
    11221 tcttacctac atcacctaaa ttaatgacat gtccaccagt catatactct aatccatggt
    11281 cgccgatacc ttcaacgaca acatctacac cactatttct aatacagaat ctttctcctg
    11341 cactaccgtt aataaatgcc ttaccacttg tcgcaccata gaatgagacg ttaccagcaa
    11401 taatttcatt ttgtcgttct tcaaaaggtg ctttgacaat gaccgtacca ccagataatc
    11461 ctttaccaac atagtcattc gcatctccag tatgatgaat cattaagcct ttcggtgcat
    11521 atgctgcaag actttgacca gcatgaccat tcgtataaac attaattgta ttttcaggaa
    11581 gtcctgcttc tccatattgt ttcgaaatct cactacctgt aataacccct acatcacgtt
    11641 gttcattatt tactgtaaag ctacctgtat agcgacgccc ttcagcaata tatggcttcg
    11701 ttacttcata taaatttgtt aaatcaaatc catgctcaag attatgattt tgttgaattt
    11761 cttttgtgtt tggcccatcg aaaggacata acagtttttc aacatcaata ctagccgctt
    11821 tgctattcgc ttttaatgtt gatgatcgtt gtaataaatc agttcttcca actaagtctt
    11881 ctacacgttt caaacctaaa gatgctaaaa tttctcttaa ttcttgtgca ataaaatgca
    11941 taaaattaac aacatgatgt gctttacctc tatataaagc acgtaaatct ttgttttgag
    12001 ttgcaactcc tactggacat gtatctttat ggcatacacg catcataata cagcccaaca
    12061 ccactaatgg tgcagttgca aatccaaatt cttccgctcc aagcgcacat gcgtacgcta
    12121 catctttacc agttaataac ttaccgtctg tttctaactt aacacgactt cttaagtcat
    12181 ttagttttaa tgtttgatgt gtttctgcta aaccaatctc ccaaggaaca ccggcatgct
    12241 gaatactcgt tttgggtgaa gcccctgtac caccatcgta accactgatg acaattttat
    12301 ctgcaaatgc ttttgccacc ccagatgcaa tggtaccaac acctgttttc gaaactaatt
    12361 ttaccgcgat atctgcatct ttattcgcat ttttcaaatc atgtatcagt tgcgctaaat
    12421 cttctattga ataaatatca tgatgtggcg gtggtgaaat cagaccgata cctggcgttg
    12481 accctcttgt cttcgcaatc cacggatata ccttagtacc aggtaattga ccaccttcac
    12541 caggctttgc accttgcgca actttaattt gaatttcttt ggcatgttgt aaataatcac
    12601 tagttacacc aaaacgccca gaagcaactt gtttaatcgc acttactttg ttgcttccat
    12661 caacttgtac ttcataacgt tttgcatctt cgccaccttc accactatta ctctttccac
    12721 ctaattggtt catggcttgt gctaacgttt catgtgcttc cgctgaaatc gatccataac
    12781 tcatcgcccc tgtattaaag cgtttgacaa tgtcacttac cggttcaact tggtcgatgt
    12841 caatcggtgt acatgcttta aattcaagta aatgtctaat gtgatctgtt ctatttttgt
    12901 tcaccgcttc agagtatgct ttaaattgcg catagtcatt ttctttacat gcgtgctgca
    12961 ataagaaaat agattccgga ttaaaagcat gatgttgacc ttgttgtctc cattggaatg
    13021 tactacctga tgcaagataa ttatcatcac tttgttgacg tgctttattt tcagcatcaa
    13081 tttgatcaat cgaaatacca gataacttag actgtgtccc agtaaaataa cgatcaatca
    13141 catcatgaga caagccaatc gcttcaaata tttgtgcccc ttgataactt tgcactgtcg
    13201 aaattcccat cttagccatt actttaatga caccttctga caatacatcc gtatatgtct
    13261 taacattatc gacaacggtg ccttgtaacc cttctgtcaa tgtcagttgt tcaactgtac
    13321 gttgcgctag gtatggcaca attgcattcg cgccatatgc gagtaaacaa gcaacatgat
    13381 gcacttctcg tgtctcacca gatttagcga ctaaacttgt agacatacgt aaatctgctt
    13441 taataagtaa ttgatgcaca tgacttattg cgagtaacat cggcattgca aagccattgc
    13501 tatcaactaa tccactatca tctaacacta gaatttgagc gccttgcttt acagcattca
    13561 ctgcttctcg gcctaatgct tctaacgcat cttccaaatc cccttcatat accgttgata
    13621 aataagttaa tttaaaatgt tcctgatcaa tcgctgctaa gtgtgattca ttcaataccg
    13681 gccttttcaa ttgaatacga tctaaaaccg tttcgtcagg tgctagtaag ttaccttcgc
    13741 cacctaaata agaaagttca ctcgttacga ttttttcacg atacgcatca attggtggat
    13801 tcgtaacttg tgcaaacagc tgtttaaagt aattaaatag tgattctggt cgctcgttca
    13861 acactgcaat tggcgcatca tatcccattg caccgatagg atccttctta ccttctacaa
    13921 gttcctgaat atacttatga atctcttctt tcgtgtatgc aaactgacgt tgtaatttaa
    13981 ataacgtctc atctttccat tgcgaatctt gatattgtat attttcaaaa tcaaagtcaa
    14041 ctttatggtt atcaatccac gctttatatg gtaattctcc agcaatcgca ccttttaaat
    14101 cattattttc aatgacttta ttctgtttaa aatcaacaag caataacttt ccaggattca
    14161 attgaccttt aaaagcaaca ttactttcag gtacgtccac aacacccact tcagatgaaa
    14221 agacaataaa gttatcttta gtaatcgtat aacgacctgg acgtaatcca tttctatctg
    14281 taagcgcgcc aagtttgtca ccgttacaga acgaaatcat tgtaggacca tcccacggtt
    14341 ccattaaata actataaaat tcataaaacg cacgtacatt tgcatcattc gcttcattat
    14401 ataaccaagg ttcaggtatg agtaacatcg ctgccttttc tggctccatg gctaacgata
    14461 agaactctag cgcattatct acaatggcag agtcactacc atcctcatcg acaatttgaa
    14521 acactttatg ttgatcctcg ccaaataatg tttcgattaa tttatgttgg cgtgctcgca
    14581 tccagtttac attaccttta atcgtgttaa tctcaccatt atgcattaac atacggttag
    14641 gatgtgccct tttccaactc gggaatgtat tcgtactaaa tctcgaatgc actaacccta
    14701 gctttgattg atataaatca tccgataaat ctgtatatag ttttttaatt tggtctgatc
    14761 gtaaccaacc tttatataca attgttttgc gtgataagct cgtaaaatac aattctaaat
    14821 cgcactgagt cgaatagaac tctaattgtt ttctcgctaa aaacaaacgc ttttcaacat
    14881 cttcaatgtc cctaatatca ataaacactt gttgaatgac tggcatcgta tctgctacat
    14941 gtttagcaat ggcatcttta ttaactggta cattacgata accaagaatt gataaccctt
    15001 cgccttcaaa atatttttta aaaactactt catgttcaga acctaaaatg cgttctttgg
    15061 aaaaaaataa ccccacggca tattcacctt cacctgggat atcaaagtcc gttacatgtt
    15121 gtttgaaaaa tgcaaaaggt atttcagtca taatacctgc gccatcacca gtgatgccat
    15181 ctgcgccgac cccgcccctg tgatctaagc gtcgcaacat ttcaagcgat ttatcaatga
    15241 tgtcgtgaga ccttttatta tccatattcg cataaaaacc aataccacac gcatcatgtt
    15301 cctcacgata gtcatataag cctttaatta atttctcatt gtgcatgatg cacacccctt
    15361 ttttagaata ttctgatatt agttattaat gataattact ataatctaaa aaatttatct
    15421 tttcaatata taaaatagaa gatattcatc taaaaaatag aacatttatc aataatattg
    15481 ctataatagt tacaaaacac tcatttttgg aggtgcctat atcgctatgg aaattaaaca
    15541 attaagatat tttatcgagg tcgctaaaag agaacatatc tccgaaacag cacttgaact
    15601 aaacattgct caatcagcga taagccgtca aattacatta ttagaacaag aacttaatgt
    15661 gtctttattt aaaaagcaag gacgcaatat tacgctcaca tctgaaggga aattattatt
    15721 taatgaagct ctgcgtatta ttgaacattt agatagcacg attgaacaat ttcaaagcca
    15781 tggattaact aaaaataaaa gtatctacat tggctatgat gagagtgacg tatcccacat
    15841 gttactacca cttatccaaa cgtttcattt acaaaatgat acacatgtca taccgagctt
    15901 attagaacat gacacaatta taaatagcgt attaaatggg aatatagaca ttggtttcac
    15961 ggaacttaca ccagaaatac gtaaacacaa acaattacat atgctacctt tattcgaaga
    16021 gcactatcat ttatatgcgc cttcagacga tccaattacg atggcgacac acccaccatt
    16081 aattcagttt gagcatagtc atatttattg cttggcacca tttgccgaaa ctgtaaaaaa
    16141 acaattgcgc aagattacta aatcagatgt atacacaatc agtagccaac cacttgcgca
    16201 gtatctcctt cgtcaaaaag aaggttacat catttcatca caaaatatac atttaccaga
    16261 atctaaatca tggatagata taaaattaga tcatactgaa ttaaaaagaa cgatatgtgc
    16321 tattacaaag gagccttata caaagagtga tattggcata ttattaacac ttattcagca
    16381 attaatgact aaaacgtcta cattccatta agtaattcga tgctatcaat gatgattttc
    16441 gttttacact tagcagtaat atctttgaaa ataaaaaatt aagggcgtat aatcaccata
    16501 ttaattcatt catagaattg gggttttgtt tttgaaatat agaaatttat ttacaaagcc
    16561 atttaatata attttaggtt tgttctgcat cgcattcatt ggtgctattt tatttacatt
    16621 caataatgag aacttttaca acaaaccaat tggtcaaatt atagatgtca aacatgtttc
    16681 ttcgacacct acaaaagatg cacaaaataa tcgtgatatt aaatataaaa atcaattaaa
    16741 agtcaaaata ttaaatggtc aatttgctgg agaaacgacg acgattaatc atcagtacgt
    16801 caaatcgcaa gcagattctg aggcatttcg cacacacgaa aaagtattat tacatatttc
    16861 taacaagccg agtgatgctt atattataga aaagaagcgt gataccttaa cggttatcat
    16921 cactggtttg ttcttattaa ctgtattatt ggttggtcgc aaagtcggac tgcaatcgat
    16981 tttatcgtta atcttgaatt ctattgccat attaatcgca atttacattc atattcaaca
    17041 tagcaatatt aatttattct tactcatgac cattgccatg atctgttcaa caatactcac
    17101 tttattgctc gttacaggct ggcatatgcg tacactcatt accatagcca gtacaattat
    17161 cggaactttt ttgagtatag gtttaacaga attaatcata tatatggcag atggtaaagg
    17221 cattaaatat gaaacgatga actttttatc attaccaccg aaagatatct tcctagcctc
    17281 agtgttaata ggttcacttg gcgctattat ggatgttgca attacaattg ctagtggtat
    17341 gcatgaaatt ttacagcgta cgccacacat ttcaatgagg agatgggcgc ttgctgggcg
    17401 aaacatcggc caagatatta tgggaacaat gactaacatc ttattattct catatttatc
    17461 cggtgcacta cctatgtttt taatcttttt aaaaaatgcc aatacagtga cttatactat
    17521 ttcgatgaat tggtcgttag aaattgcgcg cgcactaaca ggtggtatag gtatcgtact
    17581 tacaattcct atcacaatat tgtttatgga aatatttgaa acattaagga gggcgaaaca
    17641 atgaatgctg tatttatatt ggcgttattg ctcctctttc tgatgataat tttcggaggt
    17701 aaaaagggat tcatctctta tttaacccta tttttaaatt tcgctatttt aattattagt
    17761 attgtattga ttatttttgg cgtaccgatt tatgtcgtca cattcttttt ctgcattatt
    17821 attggcgctt gtaatttatt cgtactcaat agttacaatg ttaaaacaca agctgcattt
    17881 atcagcacta tcgttacaac aatattgttg attacactca tttatttatc ggtctacttc
    17941 ggtcatctac aaggcttttc aaccgaacaa caagatgaaa catatgtgta ttcaatgaat
    18001 attggcattg atatggttca atttatggtg ttcactatta ttcttgctgt tattgcagct
    18061 gtgatagatt taacaattac cattagttca cccatttatg aattgcatca agtgaatcca
    18121 acgcttacgc aatacgaatt attccaatct ggcatgcgtg ttggtagaga aattttagcg
    18181 acatctgcga acacgattta ccttgccttt tttggtggcc aacttgcttt atttatttgg
    18241 tttttcaaac ttaaatattc atttggtcat attataaact cgaaaatatt tgcccaagaa
    18301 tttatttcta ttattttagg cggtattgcc gttgcaatca gtattccgat tacggcttgg
    18361 attacggcat ttttaataaa aagaccatct cgacagcgca aaaatgatga gtctatcaat
    18421 taatcgcaac tagcaatata acttaagtta agcgacctgt ggatatatgt caattgttca
    18481 ctaggtcgct ttttactata gtaaattgtc cgatgatcta taacaaaaag cacctttatt
    18541 catcattaat tgttgtggtc gacgatgagt aaagatgctt ttaaatttaa tttattttat
    18601 agttaacgcc ataatatgaa caggtcttcc gacgaacata ctatctccca tatattgata
    18661 tccttgttgg cgataaagtg ccatggcatg tggattgtca gtattaaccg ttaacacaat
    18721 ctcattaata tcctgaaatg ttgaagtgat aaatgacgcc aatttttcca ttaccacttt
    18781 acctattcct ctattacgat aacgttgatc aacactaaat gacctgaaaa atactgcatc
    18841 ttggttatcg ctaaatggtg cgaccccttt tccacgatgt aatgtaaaaa aggccacaca
    18901 ttgcctgcca tccattacaa cgatgggata cctttcatta tcagacattg cagctgtaat
    18961 attttcctct ggcgttttaa caaacttcaa atcactttca cttaattgaa aagcttctaa
    19021 catcgaataa tcttgtggga cataatgtcg taacatgatg gtgtcattat ttgtcatgtt
    19081 taccaccata agtttcaatc caaactttga cagcaggcgc aatcaacgca tcattatctt
    19141 tatcaatcca gcgtatatcc gtaatttcat tattgatagt tacgttttcc caatcgattt
    19201 tggttaatgc gcgaaatcca tttaactcag ttaacatatc ctgttgtgga tatgcaggac
    19261 ctacaattgt cccgacatat tcaatttcat ccattgttaa tgttaaattt aattcttctt
    19321 ttacttctct taacagcgcg tgtacttgtg attccccttc ttctatttta ccacccggga
    19381 aataatactt ttcgcgattc cttacttgaa caagcaatat tttatcagct gtttcttcaa
    19441 ctaaacagac acatttaatc atctttgaca ccccaacttt gtgaaatcaa tttttcaaat
    19501 tatactgtac aattatgtta tcatatatga gtagttatag cgcaaaacgt tagcaattca
    19561 gcgcacccaa cttttcatat aaacagaaga tactaggggg aattattatt atggcaaaac
    19621 gttccaaatc acaacgttta tcaagtttac taaatgtcgc aggtttcata gtcgacggct
    19681 acaatggcta taaatatcat gctaaaaata aaaaattagt atatctttca ttaggtttaa
    19741 gcactgtagg aaccgtgtta gacttttaca tttcaattaa gtcaccacgt aagttcaaaa
    19801 aagcagtggc agttgttact ttaataacaa acggtgctag attatttaca agcattcgca
    19861 aagtaaaaca tgaatactaa ttcagaaaag gattggtcga acatagaaca tgaagttcat
    19921 tcgaccaatc ctttttcata tacaaaaatt cctattacaa caatcacgac tttgatagcc
    19981 ccgccataaa atttaggatt caatccaact tttcagcttg tgaaatgtaa taggaattca
    20041 ttatatattt atatacgtaa gactttagtg aatatatcta taattattta cttggtaagc
    20101 tggtaccgtt ctgtaagtta aaatacctgg tgcagctgaa tagttcattt ctgaaactaa
    20161 aatactacca tcgttattta cacgttctac aaacataaca tgaccatagt aacctacatc
    20221 tgtttgagcg atagaaccta cagtaggtct attgtcgata gtgtaaccat ctgctgccgc
    20281 tgcgttatcc cagttattag cattccacca ataagtacta atacctttac caatttcagc
    20341 acgacgatta aatacatgat atgtacattg accccatgta tataagtttt ggtgactgaa
    20401 tactggtgta ttgtaacctc tatttgtcgt tgttgcagat cctgagttcg tagatgcatt
    20461 accagttact ttcaattttt gacccggata tataaagaaa ttatttaagc catttaagct
    20521 cataattttt tgataagttg taccatattt tgatgcaatt aatgacaatg agtcacctgc
    20581 ttgtactgta tagtatgatc cgccacctga gttcgttgat ggacggctac tattgctcgc
    20641 agcgttactt gagctagcag tacctgatac ttttaatttt tgacctggat aaataaagaa
    20701 attatttaaa ccattaagtc gcataatgtt ttggtaagtt gtaccatatt ttgatgcgat
    20761 taatgataat gagtcgcctg cttgtactgt gtagtatgat ccgccacctg agttcgttga
    20821 tggacggcta ctattactcg tagaattact tgagccagat acttttagta cttggtttgg
    20881 gaaaattaga ttagatgtta aattgtttaa tgactttaat ttagcaatcg aaatcccata
    20941 cttatttgaa attgcccaca ctgattcacc cggttttact gtgtgagttg tagccgcatt
    21001 tgcttgagtt gccgcaacag cgctaatcgc gcttgtccca ataatagctg caattacttt
    21061 tttttgcact ttaaaatcct cctcttgctt aactttccta acattctttt atccgaattt
    21121 atgaatacta catcattata cgattttatt atgtataata ggttgatgtt tgatgacatt
    21181 atgattacaa aaaaatcata tactgtatca tcaaatttta taattacccc ttaaaattat
    21241 tacaacttat tagattttac aatatctaaa ttattacaat ttcataatat ttcactataa
    21301 aatgattaca atccctttct ctattggaaa taattttatt ctccaacaat aacgccctac
    21361 aaacataagc atgaactttg cttgtaggac gcaatataat ttattttgct aatgacgttt
    21421 ctattgcctt aatctcatct ttagataaat taacaggttt ctctccatct ttgacatctt
    21481 cctgcaacgc tttttgagct tcttttgaat gatacaattc tacgatttta gcatatgttt
    21541 tgttatccaa gtctttgtca ttaactgcaa caatattaat atatggcttt actgcatctg
    21601 aatttgattt ttctaaaaat atcggatcat ttttaggatc tttacccgct ttagttgcta
    21661 caccgttatt aataactgca atatcgacat cagataaagc acgtgcagtt tgttgtgcat
    21721 ctactgcagt aatttttaaa tgttttggat ttgacgttat atctttcacc gtgcctgcta
    21781 atccgaaatc ttttttcagt tttattaaac cagctgcttc taatagttta agtgcacgtg
    21841 cttggtttga cacatcattt ggaatgacaa ctttagcacc atctttaacc tttttgacat
    21901 ctttaatttt atctgagtaa atgcccaacg gtgctaaaac tgttgtactt aatgctgaaa
    21961 tctttgttcc tttatgcgcc tttttatatt gatctaaaaa tgcaaaatgt tggaatgcat
    22021 tcatatcaat atcaccatca tttaatgctt tattcggtaa attgtaatct gagaagtgct
    22081 taatctccac atcaatatca tcttttttag ctaattcttt aaccttctcc caagccttag
    22141 tgtcatttga tgcgacacca attgttactt ttttatcatt gttaccacca cacgctgcta
    22201 ataatacaag tgctacgata actaacccaa tcaatctttt cattctatca attcctttca
    22261 aaatcttcac tatatatcat taatgtctac gtatgaatct agctagaaca ttccctagcg
    22321 tttgaatcac ttggacaata atgactaata caataacggt aataataatg accgtcgtat
    22381 caaatctttg ataaccatac actaaagcta agtctcctat accaccaccg ccaacagctc
    22441 ctgccatcgc cgtacttcca ataagtccaa taatcgcagt ggtaattgct aatactaacg
    22501 aacctaaagc ttcaggaatt aaaaaatatc taatgatttg tagtggtgaa gcgcccatcg
    22561 ctttcgccgc ttcaataatc ccctcgtcta cttccaataa tgagttttca acaagtcttg
    22621 caatgtaagg tgccacatat actgttaaag gcacgatggc agcagtcgta ccaattgaag
    22681 tacctactac taattttgtg aatggcacaa tcgcaattaa caaaataata aatggtagtg
    22741 accttaaaat attgattaaa ggatttaaaa cttgatgtat cactatattg ggccatatgc
    22801 cttgttttcg agtaattacc aataagacac ctaatggaat accaatcact gctcctaaaa
    22861 ataaagcaat agataccata tatagcgttt cgtacaatgc ttgtaataac tgtgcactgt
    22921 ctaaatcaga accaaacata tgttaatgca cctcctcaaa ttgaatattt ttctctttga
    22981 aatattgatt tattgccgtg tcttcaaatt gttgatccat attaaatcga agccacatat
    23041 aacatacggt gttaccttgt atttctgaca tagatgaaaa taaaatttta acctctctgc
    23101 cacaaatttg aatcaagtca tttataatcg gttgtgtcac ctgagtttcc tcgacgaaga
    23161 ttttataatc tttaaaatcg ccaacttgtt cgtcattcaa tcgacgaatc aatgatgtac
    23221 ttggctcagt ctgtataact gtagacacaa aattttgagc aatcgtcgtt ttaggatgac
    23281 taaacacctc tttaacagtt cctgtttcaa ccactttccc cttttccatt acagcaacac
    23341 gattacaaat gtctttaata acgcgcattt catgtgtaat catcataatt gtaatgccaa
    23401 aggtttgatt gacattcttt aataacgtca atatcgaagc agtcgttgct ggatccaatg
    23461 cgcttgttgc ttcatcgcat aggagtattt tcggattagt aacaagcgct cttgcaatag
    23521 ccaccctttg cttctgccca ccagataatt catcaggaaa ttggtctttt ttatcactca
    23581 atcctacaaa ttcaagcatt tccgttactc gttgcttaat ttctgttttg cttttcttac
    23641 ttaaaatgag tggcattgct acatttttaa atacggtagc tgaatttaat aaattgaaat
    23701 gctgaaatat cataccgata tctttcttaa tatccctcat cattttatcg ctataattcg
    23761 taatatcatg tccatctaca atcacttgtc cattcgaggc agcttcaaga tgattcacga
    23821 gtcttaccaa cgtacttttt cctgcaccac tatatccaat cacaccaaaa atatcattgc
    23881 gattgaccgt aaatgatacg tccttcaaag catctatttt ttgcttcttt ttattaaagg
    23941 tcttacttac ctgtcgaaac tcaatcatca tcgcccctcc tacaaattct tattaatcca
    24001 ctaggaataa gagcattata tgtaaaattg catatatcgt caatacaatt tgccgaattt
    24061 tctaaaaaat taaaaaataa gtaattcatg tgacaatgac gaattgtgag actactatga
    24121 catttatcaa attaaatcca taaaaatgtc caccaatcct ccacaacgca attactaaat
    24181 attaacatcg cacaaaaaag cactagcata ttcaagaaca acaaacgttg aactcaaaat
    24241 atatgccagt gctgctatta tttataaagt atctagtgct tgttttaaat catcgactaa
    24301 atcttcagta tcttcaatac ctacagaaat tcttacaagt ccgtctgtaa taccttcttt
    24361 agctcgaata tctgctggaa tggatgcatg tgtcatcaat gcaggtactg aaattaaact
    24421 ttccactgca cctaaacttt cagctaatgt gtaatacgat gttgctttaa tcaattgttt
    24481 ggcactttct gtatttttca cttcaaatgc aatcacacct gtatggccat ccgcttgagc
    24541 catatggaca tcatgattta aatgactttc aatacttgga tggaacactt gttgcacagc
    24601 tggatgtgct tgtaacattt taataatttc aataacgctg cgattaattt gttccatacg
    24661 taaacctaat gttttaatac ccctcacaag taaatagcta tcttgaggtc ctaaaatgcc
    24721 acctgttgaa tttgaaataa atgctaaacg ttctgcaagc ttgtcatccg atgttgcaac
    24781 taaaccagca acgacatcac tatgtccacc taaatatttc gttgcagaat gtaagacaat
    24841 atcgatacct aaatctaatg gattctgata ataaggtgtc ataaatgtgt tatcaacaac
    24901 tgaaatcaaa ccgtgttctt tcgcaatttc agcagacttt ttaatgtcag taacacgtaa
    24961 taatggatta gaaggtgttt caataaacaa catctttgtt gttgggcgta tcgcttgtac
    25021 aattgaatct gtatgcgttg tatctacaaa atccacttca atgccaaatc gtgtaaatac
    25081 ttttgtcaat gcgcgataag taccgccgta tacatctgaa tttaaaataa tatgatctcc
    25141 tttgtccaac agcataacaa ctgcactgat tgctgcaaca cctgaactaa atgcaaagcc
    25201 atgtttgcca ttttctaatg tcgcaataac gctttctaca gaacttcttg ttggattcgc
    25261 agtacgagaa tattcatatc cttgacgtaa atcaccaata tcatcttgta aatatgtact
    25321 tgtttgataa attggtgttg taacggcacc tgtataatcg tctgttgtgt gcccaccatg
    25381 aattaattta gttttcttgt tcattattat tctcctcata attaaatatt tgcttagaca
    25441 tatatcgatc actaccatct ggaaatacga caacaatcgt accttcagat aattgcgctt
    25501 ttaaattcaa tgcaccttgt aatgctgcac ctgaagaact gcctactaac aacccttcat
    25561 ttatagccaa acttttgaca tttcgaaagg catcttgatc tttaatcgta aatatcccat
    25621 ctacaagacg tctctctaaa aatatcggcc atttctcaga accgatacct tcagtgtcat
    25681 gtgcatgagc tggccctcca tttaacacgg acccttctgg ctcaacggca taacattgca
    25741 cgtgatgttg ctttaaataa cgtgcggtac ctgtaaatgt accgccagag ccaataccag
    25801 ccacaaaata atcaatttgc tgtaatgctg aagtcaattc gggtcccaat gtatgaaaat
    25861 atgtatccgg attatgttcg gattcaaatt gattcatata aacggcacca tatttttcag
    25921 cataggaacg tgcagctaat tgtgccccat gcataccttc agactgactc gtccttgaaa
    25981 cttctgcacc aagcgctatc ataatattaa tcttttcttc tgaaaaacca tacggcgcaa
    26041 agatcttaca tttcaaatga tgtctattcg ctgcaatagc taaccctatg cctgtattac
    26101 cagcagtcgc ttcaacaata gtttgacctg cacgcacacg cccttcttga attgccttct
    26161 ctactaaata tttcccgagt ctgtctttaa cactgcctcc aggattccat tgttcaagct
    26221 tggcataaat tttaacttta tcatcactat aatgttctaa cagtactaat ggtgtattgc
    26281 caattaaatc ataagtaatc atagatgcac cctcatctga catgccgatc aaatgaatga
    26341 aacctttctt catgtctcaa ttttaattct tacttttcag ataagaatta taaacgacat
    26401 tttgttattt tgcaattatc taagtttcga ttaattcaga accagtacta aattttcaat
    26461 tccaaacaaa aaaacacctg agcaacacaa atacttgtgt gtcagatgct tctatatatt
    26521 aactaaataa ttgcacgata aagactaaaa taataacgac aggcatcgca tacttaatta
    26581 agtaatacca accactgaat aatctaaatc gatctttacc aaaatattgt tgtaataatt
    26641 ttttatctaa taattgtcct acgacaagcg tagtacctaa tgcgcctaat ggcatcaata
    26701 cattcgaaac gatgaaatcc atattatcaa aaatcgttcc cgcaccgaat cttacatctt
    26761 ttaagatacc aaaagataag gttgctggaa tactaatgat aaatactaaa atactaccga
    26821 tcactgcgac ttttttacgt tttgtattgt cattcttcgt gaagttagaa acatttaatt
    26881 ctaataaaga aatagatgac gttaaagccg caaataagaa cagcactaag aatcccaaat
    26941 agaataatgt gcctagatgc atttgactaa agaccattgg cagtacttta aataataatc
    27001 caggcccttc ttgtggttca tagccaaaac tatgtaaagc cggaaatata gctagacctg
    27061 ccaatacaga tacaaagata ttcataacaa cgatagaaat agctgatgac ttaatcgtca
    27121 tgtctttaga ggcataactc gcataagtaa tcatacctgt agttcctaat gataacgtaa
    27181 agaatgattg acctagcgca aacaagatgc catcagcagt aatctctgat actcttggtt
    27241 gtaaaataaa tttcacacct tctaaggcgc catctaatgt taaagactta atcacaatga
    27301 cgattaaaaa gacaaacagc aatggcatca taactttcga tgccttttct aatccttttt
    27361 caacacctaa catgacaata atcatcgtag cgaatatgaa tataccttgc cctagaacgg
    27421 ttaaccaagg atttgatatt accgcttcaa aattcatttc ttggagatga ttgatgcgtt
    27481 gaaatataac taattgccat aatacttgtc cgatgtaaat gacaatccaa ccaccgataa
    27541 cactatagaa gccaaataaa ataaacactg ccaaattacc gttccagcca atgatattga
    27601 gccatttttt accagttaat ttactatata tttgtgttgt atatgtccgt cccattttcc
    27661 caacagtgaa ttccataatg agtaatggca acccaacaaa aatggtgaat attaagaaca
    27721 tagctagaaa ggcaccgccg ccataaatcc ctgccatata tgggaatttc cacatggcac
    27781 caagaccgat tgcagaaccc gcactagcta aaataaatcc agttgatgac ttccattgtg
    27841 attgttgtct tttcatcatt cacatctcct ttaaagcgtt aaagcgtcta cgtgtttaag
    27901 taacactcta acatgtatgt aaataacatg caatacacct gcatcaaaat tgtttcatta
    27961 atacggtagc acctggattt atcataatat aatcaactct tgtaatgtgt tgtcattacg
    28021 ctacattaaa ttttctggtt caacgctgta tacgtgcgcc aatatctcgc cacatacaac
    28081 accatctata tccccaacat ctcgccacat aacaacttca ggtgcaatgc ccataaaatt
    28141 tccacgacat tacaatacaa atcaacttct ggtataactc cataatattt ccacgacatc
    28201 acactacaaa ttaactttag ctaagttccc tatactatca cgccacaaaa aacactcaaa
    28261 tgacaaatat aataacatcg tcactcaagt gttaattttt aaaatcttca attatgttaa
    28321 agcattgaaa aagcgaataa cattttcttc taaaatatct ctgccttctc catgcgtcat
    28381 taaatgatgc gaatccttca gaccatttaa ttctttgtct actgatccta aatgttcata
    28441 aataaaatgt gcacttgttt cataggatgg cgcatcttta ccaccaaata atatattagc
    28501 tggcatttta ataacattta aatttgtcat gatatcatca ataaaatgtt gaaacttcgt
    28561 aatttcacct tcataatcat caattgctgc taattgcgca ctcgatgctt gctgatctaa
    28621 atttaaaata ttcgacatgc gttgactata ataaactaaa tgttctatta agccatcgtc
    28681 actcttttcc tttggtgctg acatgacagc gatacgtttc aaaggatagt gttgcgccaa
    28741 ttttaatgtc attaatccac ctaaagacac acccgttgca ctgatagatt cataaccttc
    28801 attgactaaa aattggtaag ctttctcaac ttcttcccac caatcatcta cattatatgt
    28861 catgaaatct ttcaacaata aaccatgacc tggataattc ggtgcataac aactaaatcc
    28921 ttggtcattt aactcagctg caagatgctt cacatcccga tttgtacctg taaatgaatg
    28981 taataataat atcgcatgtc catttgtgcc ttttaaatac gatggactcg gtgttttaat
    29041 tctcattttt ctatatacct ccactatgtc taaagatgtt tgctaaacgc gttgtcgtcg
    29101 atgattaata aagtatgggt ataccattaa gaataacgct acccaaatga ttgctagtga
    29161 cgtgccgcca atgacatctg aaaagtaatg tgcatgaaaa taaaggcgac aaaataatat
    29221 gctaagccat aatattccca taaccaacgc actcaacact tttgttattg tcttagcagc
    29281 aagtgaaata ataatgatca ttaaggcgaa atataataat gtgctggcgt tggaatgtcc
    29341 actcggaaat gaaaagcctg tatcaacggc taaatgatta tatggtcttg gacgtaatac
    29401 agtatcttta attaatttgt tcatgatgac acctgaaacc aaatatgtca caaaccaaac
    29461 cgctaaatgc ctctgtttaa taaacagtat gattgtgacg ataatggaaa tcaacacgac
    29521 acctttgaca tctccaattt ccgcactaaa cgtcatatag taattaaaca aattgttaac
    29581 atactgacgt tgtggctcac cgaaataatc tgtaaaccat gttaatgatc ccatatctat
    29641 atttttaagc cattcttgat ttgtcactac actgtaaaac atacctataa atacaatcag
    29701 cgcgattaaa aataaaggca ctgtcatttt cggtgatgtt aattttttat ctatcatctt
    29761 acaatctcct cgtatcatca ttttcatttt acaaatgtta tccataatat caatgtgcca
    29821 caaatttcac tttaccgaca atatcaaaat tataaagttc atattgttat gtatattgca
    29881 aataaaacat tgtataattg aaataacaat attttgctat tttcaattta gtacgattta
    29941 tatttattat acagaggggg taaggcgtat caatagagtt atttttgtct atatagcgtt
    30001 aatcattaca ttagttagat tctttacccc tattcatcca tcattttcaa atttgattta
    30061 ctggatattt gtattatatt ttattcctat tatactatgc gttatcggtt tcaaggccga
    30121 aaaacttatt gcaacaatgg tcattatacc taatttttta ggaatacttt atcgattata
    30181 tgcctacgtc acacatatac tctttatgta aaaggatagt gggcatgtct cgcaacaaat
    30241 atgcttgcgt cgacttgtca ccattcgcaa attttatgac atacgccttt cacgggctat
    30301 attcagaccc acgcattcat ccacgtaata aacacatcat gtaatagaaa aacagcacac
    30361 ccaaatatat ggcgttgcgc tgtttaacca agcatacttc tatagcttta ataagccagc
    30421 agaagcatac ctaaccttct taaatatgct tttccaaatt atcctcaagt ttgaatacga
    30481 taatacgttc acctgtaact gtacttaaat cactatggaa gctcatcact ttgatacctg
    30541 taattttaaa aatgatatca ttcaaatctt gctcaccgga ttcaactaat tcagaacgtg
    30601 ttcgtttaat atttaataat ccttcattcg tactacatac acgatattca gctggcgtta
    30661 agataccttg taaactaata atcaccatat ctcttaaaat gtctgattta actgacaagg
    30721 aacctctgcc aaggaaatct ttttcccatt gcgtaatggc tttactgatt tcagcttcga
    30781 tttcaccttt cgttcttttc atatcactaa ctccaataat attaaaattg attacttcat
    30841 ctttgtatcg ttatcacgac atcaactatt acattaagtt tatcattttt agtatatttt
    30901 aagaagctag aacattgtag atatgatgat atattagtta cttagcatcg caacatatca
    30961 tcgttaaatc caacttttaa aacgcccttc ctcattaacg ctcattaaac gcagccaatg
    31021 attagacacc ttcctagcga aatgctcatt attcgcgagt agtcttgcta caacatagtc
    31081 gggtgcctga ataacgacaa gtaaacgaat tggcgaatga tacatcgtcc gatcagcagc
    31141 cataacagat tgccatgata agccatacat cagatcactc gcattacctt gcatgacacc
    31201 aacacctgac gtgacggttt gtgtcgcttt attcccactt ccgtaaaaat gcggcgcaac
    31261 tgtcgacgca taatattgta aattaatcca ttgtgccaca agtgccggac cagaaatgat
    31321 ggtatttaat aatgtgccat ctttatcttt acgccaatca taattgtgta aaaatgtccg
    31381 cccttctaaa tcaatgcctt ttgttaattg gcgtcgtcca attataaatg atgcattttt
    31441 agccaatccc cattctggac gtacctcact ccaatcactc gcaaaccgct gcgcttcttc
    31501 cactggatga ttcacacgac caatcgttgg cagtttgtcc aaacgttcgc gattcgcgtg
    31561 ttcagaaatc atcggcatcg cgtcattcaa tgattcatat gcatctaaag caatagaaga
    31621 taatgtgtct ggcacatata cccatgccaa cgtatcagta gacgtatgat gttctgctac
    31681 cgcaaaaaca gttgtctctg gaatatacac acctgattgt tttaatcctt gtctgacatt
    31741 tggacgatta catatcatcg ctaataactt agcattaaaa ccgcttgatg cgccaccaca
    31801 agccccacat tcaagtgatg catgatgtgg attattgtga gaatgactag catgacctgc
    31861 taacacaacg aacggcgcaa atgcttcggt taaatccatc aatttcaacg cttgtaacgc
    31921 gaaatcaatt tgctcttgct cagtaaatcc aacaggtaag tctgatgttc ggtcaaactc
    31981 acgatcaatc gtcaactttg tttcaggctt tttcaaccac ttttgtttta ttttttgtaa
    32041 agacgcgcga ctttttctag gcataatcga attgacaatg gtacttaagc ttaaaaatgg
    32101 cccacttaat tcaggcaata acagactagg catgacatta tttttcatca atttaaatgt
    32161 gtaaaacatc gatgacattg tctgttgctg ttgtcgataa acattcatat cgtagcggtc
    32221 tgcaaattct ttaatgcgat atgccggcgg taccatgaca ggtaatgaat catgtttgaa
    32281 ttgttcgtct acggcatctt tttgaatagg taatccaaag aagcctgcaa taccaatcgt
    32341 ttcaaagggc cctgctgctt cgatatgtct acgaaatggt tctgaacgaa catctataca
    32401 aaatgcaatt tgcgctttcg ttgatgtgcc catctgattt agctcgctat tattttcatc
    32461 aactgcttgt gtgtcattta acaatactga atgtggctga ttagcgttat cattttctga
    32521 gacatttact tggtttacat ctaatgcgcc cgccacactt tcatgacctg ctttaatttt
    32581 ttgttttaac tgagattcgt atgtcatttc ccaggcaatt agccataaat ttttaaatac
    32641 atttttattc atagttgctg caaaatgaat aaacgtttga atttcattga cgtcatgttg
    32701 tagtaataca tcgctaggca tatcactgta gtaacaccat gatgcaacag tttgcttaaa
    32761 ccaattttcc gatctacttt cacaatcttt agcgactgac ttaaactcat caccaactag
    32821 caattgttcg acaactaacc gaattgccaa ataatccgtt aacaaatgtt gttcaaagta
    32881 atgctgttgt gaacggtaat acaacatacc tgcccaaccc ggtaacgcca aaagatgtcc
    32941 ttcaacataa gcttggtagt cttcctgatc tattgaaaaa tgagttaata ctgactctat
    33001 cgtcatttca ggatcattgg gtaagccttt aatcacttgg cgctgtgctt tagtaaaact
    33061 atggtcatgt tgcgctaaat gcaaccatgc atggtaaaaa ctttgctcac gcttcggcat
    33121 tgtccaactc gatagaaatt gatcgatata aagtttcgtc catttaatca tttgacgatt
    33181 cacttgttcg ctaagtggct caccttgttc atctattatt gcatcactca tcggacgtac
    33241 atcatagtga tgatatgatt cagccatatc acgttttgat ttttctaata gtagatcagc
    33301 aacaacatca acatttgaat gattcatata tgatgcaggt acgtctttta atgttttaat
    33361 gttatcaata taaagattga tgtagtgttg cgggatattg tagtgatgtt caagtaacat
    33421 atcagtaaca agttgattaa agacactttc atctaattca ccacgtgcca cagcgctttc
    33481 tattaatgct ttatttggga aaatatccac atctcgaaca tcacgtaacc attttgcgac
    33541 atcttcaaac gtatccgctt ctaatccttc ccatggattt cgtgctgcaa aaatcgaaat
    33601 tggtgataat ggtgtaataa cacgtttcgc attttcaatg actgaattga tatttaactg
    33661 tgttgtcata cctttcacct cctataaata cttcttcaaa taattcggat gactttctat
    33721 cgctttcgag cgtgcttcac ctagattaac taaccacacg tacaataccg caaaagcctt
    33781 agagtatcga tgccgcgcca cccaaatact taataaactg ccaaagatta aaataacaac
    33841 actaatgatg acactcactg taggcggcgt tgtcgcatgt gttgttatat tttgtaatac
    33901 agcgtaaaaa taattatgtg tgatgacgta gataaatgtc acgattgcaa tcaaaatcat
    33961 accaacaaga cgtgccatgc gtcctttact aaaggctacc atttgattcc aagatacaag
    34021 taatgaccat cctagaatga gtgcacttaa cacttcatat gcacttctgt cactactcat
    34081 ccaaaataga aatgccacga taatagctaa tacacgtccc atgacaatcc agccataagc
    34141 gtctttagca gatgcttgtt ttggaatatt gaatcgcttc acgatagaac ctgattgtaa
    34201 aaataatgtt gctttaaaaa taccgtgcaa tattaaatga ataatcgctg ctgaatatac
    34261 acccaatgca cattgaacta acataaagcc catttgactc atcgtagagc ccactaattg
    34321 gcgtttatag tcaacttgaa ctaagctaat acccgatcct aacaatacag aaatactaga
    34381 aaggataagt aataatgata acgcaaatcc attatcaaat atcggcgcaa aacgagttag
    34441 aataacacca cctgcattca caattcctgc atgcataatt gccgatactg gcgttggtgc
    34501 cgttacagat tcaatcaacc atcgatgaaa aggaaattgt gctgccggta tcatgacagc
    34561 taatacaagt agtacattcg tcaacaatga ccatgtcgga tgaactatat gttgtggtac
    34621 ccgccactcg ccagtcgcaa tataaatagt tacaattgct ccaacgaatg caagccaacc
    34681 acataaaaat gtcatgcttg ataatttcgc agactcacgt ggcactttcc aaaaacgatt
    34741 aacgttcatc agcaatgtta aacataataa tgtaataccc cagcagagtg ccatcagtct
    34801 taagtcttca gacatccatg ctaaagatgc aaacgacgta atcgcagtga acaatggaaa
    34861 gtaatgtcta taatgatgat cacctagtaa atatcgcatt gaaaactttt gaataataaa
    34921 gccaagcgcc attacaaagc cagctaataa ccaagataaa cgatctattt taaatggacc
    34981 taagacatgt tgaccatgaa taccgaaaaa gccaatgact gcaaataata ctggcatgac
    35041 tagtatgtat aaatgtaatt taatatatct cattggcata actggtgcta aaaacaacaa
    35101 gccacttatc aatgcaatga taagcgcaat aacaaacagt gaaaatagca attgaaaact
    35161 taacactgca taacctcctt atttctaatc tctcgcataa ttgcttatgt ataaaaataa
    35221 aaacctacaa tagtagattc tgtacataat ggcagaaaat ttactattgc aggtttcagt
    35281 ttaactagac actgcatcac ggtacgttga tataccttgt tgcagtgttc tctttaagcg
    35341 tgctcccatg cacatatgta tataaaatgt tacttctgtc tgttcaattc atcttcataa
    35401 atatgctttg cctagacgag acctaacgtg ttattcgttt taaacttata acataaaata
    35461 taattaaatt tctgcttcat gtcaaattca tgagcttaac ctctattaaa ccaatgattg
    35521 taaagatttt gtaaatgcac ctgtacagtt aggcagtatt tcccgtcctt ttaaaataaa
    35581 aaattcgcag ttatgatcat aacaattcaa gttaggaaaa aaatcaatta cgcacaagat
    35641 aactatgtac aatgaagtta actcataagc aaaggaggta atcttaatgg gtatcatcgc
    35701 tggcatcatt aaagttatca aaagcttaat cgaacaattc actggtaaat aagatttcat
    35761 aacaaacaaa ggaggtcttt cacatgggta tcattgcagg aatcattaaa ttcattaaag
    35821 gattaattga gaaattcact ggtaagtaag ttataaaaat ctcatagata tgaacatctt
    35881 atttgaaggg ggccattcac atggaattcg tagcaaaatt attcaaattc tttaaagatt
    35941 tacttggtaa atttttaggt aacaactaat ctcaaacatt aacgatcaac aactcatcac
    36001 tatgttaaat caacatacag gaggacaaaa cgatggctat tgtaggtact atcattaaaa
    36061 tcatcaaagc aattatcgac attttcgcaa aataatttaa gcgaattgaa tacttaaaat
    36121 tctcaggcca ctataccaat agggatggtg gctttgcttt gtgttgattt attgatgtga
    36181 ggtgagtctt gttagtttgt tgcaaataaa tggtcttggt gttttttgta taggacgttc
    36241 ttagtgggac atacggaata ttcgtgatct ttgtagtctg acgcgttata tttttgtggc
    36301 gtgttttatg tttgatactc gagttctgag acattcatga tttggcatgc gaaatcttaa
    36361 tgattttcat gatctagcgc aagatatatt ggccacgtgc ggaattgcgt tgcacgttta
    36421 gactgaaaca ctcgtgtgac cgtaagtgtt aatagtacat tgatagctgc atttacttca
    36481 ctcattttta tgactgttaa acaatgattg taccttcaat taacagttgg tacgatggtt
    36541 ttgccatttt tcatcaacgt aaatataaaa aggactaaga cacatacatg tcctagccct
    36601 atggataaaa tgcaaatttc tgctttatca aaactatcac actttagata gattgaaaac
    36661 aaaaagatcc taagaacacc ttaacttttt attaattgtc ataaattgca aacaattaag
    36721 ccacaattca aaaatgatta tacttcattc aacttatcgt gctggtctaa tttgccattg
    36781 atatggatct tcaaattgtt gccaatctgc atcaatttct tgcgcattga ctaagcatgc
    36841 gtcgagttct tttgttaatt tttcttcatc taattctgta ccaataatga caaattgtgt
    36901 atgacgatcg ccatattctg gatcccattc agctgcgaca tcttgacgtt ctgctaatat
    36961 ttgtgtttgt tgcgcttcag acatactagc cacccaatat gtaactggat gaatattgca
    37021 agatgaccct gcttgagata ataaacatgc tacgtgattg tattgtgcta gccatacgat
    37081 accttttgat cgaacgacat tatttggcat gctttctaac caatcattga accttttagc
    37141 atggaaaggt agacgacgtt tatatacaaa cgatgatata ccatattctt ctgtttcagg
    37201 tgtatgcgat gcatgcccac cagactcaag ttctttgatc catcctgctg actcgctcgc
    37261 tttttcaaaa tcaaaacgct gcgtattcaa gacttctttt aaatctactt cagaatttgt
    37321 tgtcttaata attttagcag tcggttgcaa tgcgcttaac attttttcta acttcgctag
    37381 ttcttcttca ctaattaaat caattttatt aataatcaat acatcacaaa attcaacttg
    37441 gtcaattaat aaatcagcaa tcgaacgctc atctgtttcg tcaacgcttt gatcacgatc
    37501 catcaataaa tcttctgagt tgatgtcatg tacgaagcgg ttagcatcca caactgtaac
    37561 cattgtatct aaacggcaaa tcgctgtaag atcaatgcca agttcatcat caatatatga
    37621 gaaagtttgt gcaacaggta ctggctctga aatccctgtt gactcaataa caatttgatc
    37681 gatgccacct tttttcacta aacgctcaac ttcttttaat aaatcgtctc taagtgtaca
    37741 acagatacaa ccattagaaa gttcgactaa tttttcatct gtacgcgata gtcccccacc
    37801 atctgcgaca agatctttat cgatatttac ttcactcata tcatttacaa ttaccgcgat
    37861 acgtcgacct tctcgatttt gtaaaatatg atttaacaac gttgtcttcc ccgagcctaa
    37921 ataaccactt aataccgtaa ctggaatttt agccatatat ataccctcac ttcaatttat
    37981 ttgtaaatag gaataattcc gttttacatt atataggagc gtttcctctt tcgcaatctt
    38041 cgataataaa aaaatagtat acttaattaa attattgagc gctttacttt ataatggaga
    38101 caaagatata tctcacgaaa gagaatcgag gtgtataaac atgttatttg tcattttagt
    38161 tttatatgtt actggtattg catttattct actcagtgtt tttggttcaa agactgaagg
    38221 attatctacg aaacatactt tatataccat tggcagtgct attataacga ttgctatttt
    38281 catttcaatt ggctatgcca ttcaatactt aactgcagcg ctttatggtt tgtaaggtga
    38341 aggtgatgag taacgggtag ttcgggagag gttaacttgc gttgattttg ataaagtgat
    38401 catagctttt agtacttgag gatttttatt gttgctgtta cgaatgtggt catgtttaat
    38461 gcgggacagt aatttaagtt gtttttttac aattgagagt gtgatatttc gattcggttc
    38521 gaattacttt acatgggaat aatataaatt aaaaagaagc ggcctagtgt cagttgtgaa
    38581 tatactgaac attggtcgct ttatttagta gtatgatatg tagtttagct attaattttt
    38641 ttcaggtcat ccttaatgct gtctatctca gacatggcac ttttaaccca atctccttga
    38701 gctgcacctt taaaattagc tttaaaagct tcgcaatgtt gcgccatttg ttcaattaat
    38761 actttttctt caccttttaa tccgttttca atatctttgt atttatgctt atgttcaggt
    38821 gcaataactg tgcgaatatt ttctttttgc gcttccattt tagatatgag attaagtgtt
    38881 tctactgtag tacttatatc tggcattctt aaggtcatat ctggttctat tagagtcatt
    38941 taatctcctc caaattatca gtcacttagc ttatctaact gcttttcata agactttttt
    39001 aagtcttctt tatattcttc taatttccat tcttgctttc taattatctc attgatttct
    39061 tgctcatacg cttctatatt tctcgtcatt ttcctttggg catcttcact ataattcatt
    39121 tcgtgcttca aatattgaaa aacatcataa ctacgctcat tttcttttcg agttctatgc
    39181 ctaagttcat ttaaattttg cagttcattc tctatacgtt ttttctcaga ttcatatgat
    39241 tcttctaatt catttttatt attaatttga ttaaacatca tagtaatcca cctatttgtg
    39301 atgcaagcac ttgatcttta tcaacgattt cattaatact gagtctaact ctcataatat
    39361 aatttgatac agaagaccct atcgttgaaa gttgtctttc ttttgcagat atcatttgtt
    39421 caggttctct taccatacta ctttcattca cttgaccttc atctaaagca ttgagaactc
    39481 catttacact caaatcttca cctacagctt ttccagcttt ttgagcgtct tcccaatttt
    39541 ttccaaattt ctttttcttt tcattataca tttttttgat ttgattaatt tcatcgtcta
    39601 tcagttgact gaatgactgc gcaattgtta aagctgttaa actttctaaa actttttgct
    39661 gcgaagaaga taatcctcct ccatttgtag tcatcatatt tgctctaagt aattctatac
    39721 tagctaattt agagatagca ttatttgaat tctttacaaa tgacttatta ttttcttctg
    39781 cataaccttg tagtttttta agttcttttt ttatagcttt ttgttcttct tcggtcagaa
    39841 agccttccat ttcatgacct ttaccatttt taaagacttt atcattaccg aaaaaaacat
    39901 aatgcttata tttcttcaga ttttctgtca atgcatcatt ttctgcaaca aacctagtaa
    39961 tattaccttt ataattttta tataattcta ataaacgttt tccatctggg gaattaggat
    40021 tcaaactagt tatagaaata ggagctggat tataaaccac aatgttcgga ataccattac
    40081 tcattcctag aactaccgca tctcttccac ctagtgaatg tccagtaata aaatcaatgt
    40141 catacttact ttttatttta tttataaatt cttgtgtatt cgcatatcta tagttttgat
    40201 cagatgcagg agaatataat attttaagat ctgcatatcc atctttaact gtttccaaag
    40261 cattggtaac attttgtctt gaaaattcac cttcttttaa ctttttaaag gcttcgtctt
    40321 gtaaattagt cccagtcatc ccgagagtta ctttgcctgt atctttattt aaaaaagcag
    40381 aagttgttac tccagtaaga gaatcagtgt aactatcaac atattcaaga ttagaaggga
    40441 attttttcat ttttttcaaa tcattaaatt gattaatcac gtctcttttt gatagaccat
    40501 tattattagc attctcaact tcgtatgacg ctttcgaaat agcacttgta gactcagtat
    40561 tttgattaat tgcttttaaa tctattttat acttcctact caacattctc accttcagac
    40621 ggtccaaaat tcaaataatc tcttaatgtt gtc
//
LOCUS       DAKTIO010000003        39851 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.003, whole genome shotgun sequence.
ACCESSION   DAKTIO010000003 DAKTIO010000000
VERSION     DAKTIO010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 39851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39851
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.003"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(43..1953)
                     /locus_tag="O0293_000061"
     CDS             complement(43..1953)
                     /locus_tag="O0293_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0278975.1"
                     /translation="MENLYLIKDLGALAGRDYRAKEIQNLQRIEQFALGLTTEFKLHQ
                     KAKTMQHFAEQIYYNGRSQAAVNKSLQSQINALVVAPRNNSANEIVQARVNVNGETFD
                     TLKEHLDDWETKTQINKEETIRELNKTKQEILDIEYRFEPDKQEFLFVTELAPLTNAV
                     MQSFWFDNRTGIVYMTQARNNGYMLSRLRPNGQFIDSSLIVGGGHGTHNGYRYIDDEL
                     WIYSFILNGNNENTLVRFKYTPNVEISYGKYGMQDVFTGHPEKPYITPVINEKENKIL
                     YRIERPRSQWELENSMNYIEIRSLDDVDKNIDKVLHKISIPMRLTNETQPMQGVTFDE
                     KYLYWYTGDSNPNNRNYLTAFDLETGEEAYQVNADYGGTLDSFPGEFAEAEGLQIYYD
                     KDSGKKALMLGVTVGGDGNRTHRIFMIGQRGILEILHSRGVPFIMSDTGGRVKPLPMR
                     PDKLKNLGMLTEPGLYYLYTDHTVQIDDFPLPREWRDAGWFLEVKPPQTGGDVIQILT
                     RNSYARNMMTFERVLSGRTGDISDWNYVPKNSGKWERVPSFITKMSDINIVGMSFYLT
                     TDDTKRFTDFPTERKGVAGWNLYVEASNTGGFVHRLVRNSVTASAEILLKNYDSKTSS
                     GPWTLHEGRIIS"
     gene            complement(1969..2259)
                     /locus_tag="O0293_000062"
     CDS             complement(1969..2259)
                     /locus_tag="O0293_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0278976.1"
                     /translation="MATEEVKIKALLENDKQYFPATHWKAINGIPYAGSSDIDGLPQD
                     GIISVDDKNKLDKLKIGEAGIIQNSIVQKSPNGKLWKITVDDSGKLGTVLFY"
     gene            complement(2259..3842)
                     /locus_tag="O0293_000063"
     CDS             complement(2259..3842)
                     /locus_tag="O0293_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCY0278977.1"
                     /translation="MDYHDHLSVMDFNELICENLLDVDYGSFKEYYELNEARYITFTV
                     YRTTHNSFVFDLLICENFIIYHGEKYTIKQTAPKVEGDKVFIEVTAYHIMYEFQNHSV
                     ESNKLDDDSSETGKTPEYSLDEYLRYGFANQKTSVKMTYKIIGDFKRKVPIDELGNKN
                     GLEYCKEAVDLFGCIIYPNDTEIGFYSPETFYQRSEKVIRYQYNTDTVSATVSTLELR
                     TAIKVFGKKYTAEEKKNYNPIRTTDIKYSNGFIKEGTYRTETIGSKATINFDCKYGNE
                     TVRFTIKKGSQGGIYKLILDGKQIKQISCFAKSVQSETIDLIKNIDKGKHVLEMIFLG
                     EDPKNRIDISSNKKAKPCMYVGTEKSTVLNLIADNSGRNQYKAIVDYVADSAKQFGIR
                     YANTQTNEDIETQDKLLEFAKKQINDTPKTELDVNYIGYEKIEPRDSVFFVHELMGYN
                     TELKVVKLDRSHPFVNAIDEVSFSNEIKDMVQIQQALNRRVIAQDNRYNYQANRINHL
                     YTSTLNSPFETMDIGSVLI"
     gene            complement(3851..4675)
                     /locus_tag="O0293_000064"
     CDS             complement(3851..4675)
                     /locus_tag="O0293_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HCY0278978.1"
                     /translation="MQSFVKIIDGYKEEVITDFNQLIFLDARAESPNTNDNSVTINGV
                     DGILPGAISFAPFSLVLRFGYDGIDVIDLNLFEHWFRSVFNRRHPYYVITSQMPGVKY
                     AVNTANVTSNLKDGSSTEIEVSLNVYKGYSESVNWTDSEFLFDSNWMFENGIPLDFTP
                     KYTHTSNQFTIWNGSTDTINPRFKHDLKILINLNASGGFELVNYTTGDIFKYNKSIDK
                     NTDFVLDGVYAYRDINRVGIDTNRGIITLAPGKNEFKIKGDVSDIKTTFKFPFIYR"
     gene            complement(4675..10875)
                     /locus_tag="O0293_000065"
     CDS             complement(4675..10875)
                     /locus_tag="O0293_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCY0278979.1"
                     /translation="MNEKVEGMTLELKLDHLGVQECMKGLKRQLGVVNSEMKANLSAF
                     DKSEKSMEKYQARIKGLNDRLKVQKKMYSQVEDELKQVNANYQKAKSSVKDVEKAYLK
                     LVEANKKEKLALDKSKEALKSSNTELKKAENQYKRTNQRKQDAYQKLKQLRDAEQKLK
                     NSNQATTAQLKRASDAVQKQSAKHKALVEQYKQEGNQVQKLKVQNDNLSKSNDKIESS
                     YAKTNTKLKQTEKEFNDLNNTIKNHSANVAKAETAVNKEKAALNNLERSIDKASSEMK
                     TFNKEQMIAQSHFGKLASQADVMSKKFSSIGDKMTSLGRTMTMGVSTPITLGLGAALK
                     TSADFEGQMSRVGAIAQASSKDLKSMSNQAVDLGAKTSKSANEVAKGMEELAALGFNA
                     KQTMEAMPGVISAAEASGAEMATTATVMASAINSFGLKASDANHVADLLARSANDSAA
                     DIQYMGDALKYAGTPAKALGVSIEDTSAAIEVLSNSGLEGSQAGTALRASFIRLANPS
                     KNTAKEMKKLGIHLSDAKGQFVGMGELIRQFQDNMKGMTREQKLATVATIVGTEAASG
                     FLALIEAGPDKINSYSKSLKNSNGESKKAADLMKDNLKGALEQLGGAFESLAIEVGKD
                     LTPMIRAGAEGLTKLVDGFTHLPGWVRKASVGLALFGAAIGPAVLAGGLLIRTVGSAA
                     KGYASLNRRIAENTILSNTNSKAMKSLGLQTLFLGSTTGKTSKGFKGLAGAMMFNLKP
                     INVLKNSAKLAILPFKLLKNGLGLAAKSLFAVSGGARFAGVALRFLTGPIGATITAIT
                     IAYKVFKTAYDRVEWFRNGINGLGETIKFFGGKIIGGAVRKLGEFKNYLGSIGKSFKE
                     KFSKDMKDGYKSLSDDDLLKVGVNKFKGFMQTMGTASKKASDTVKVLGKGVSKETEKA
                     LEKYVHYSEENSRIMEKVRLNSGQISEDKAKKLLKIETDLSNNLIAEIEKRNKKELEK
                     TQELIDKYSAFDEQEKQNILTRTKEKNDLRIKKEQELNQKIKELKEKALSDGQISENE
                     RKEIEKLENQRRDITVKELSKTEKEQERILVRMQRNRNAYSIDEASKAIKEAEKARKA
                     RKKEVDKQYEDDVIAIKNNVNLSKSEKDKLLAIADQRHKDEVRKAKSKKDAVVDVVKK
                     QNKDIDKEMDLSSGRVYKNTEKWWNGLKSWWSNFREDQKKKSDKYAKEQEETARRNRE
                     NIKKWFGNAWDGVKSKTGEAFSKMGRNANHFGGEMKKMWSGIKGIPSKLSSGWSSAKS
                     SVGYHTKAIANSTGKWFGKAWQSVKSTTGSIYNQTKQKYSDASDKAWAHSKSIWRGTS
                     KWFSNAYKSAKGWLTDMANKSRAKWDNISSTAWSNAKSVWKGTSKWFSNSYKSLKDWT
                     GDMYSRAHDRFDAISSSAWSNAKSVFNGFRKWLSKTYDWIRDIGKDMGRAAADLGKNV
                     ANKAIGGLNSMIGGINKISKAITDKNLIKPIPTLSTGTLAGKGVATDNSGALTQPTFA
                     VLNDRGSGNAPGGGVQEVIHRADGTFHAPQGRDVVVPLGVGDSVINANDTLKLQRMGV
                     LPKFHGGTKKKDWLDQLKGNIGKKAGEFGATAKNTAHNIKKGAEEMVEAAGDKIKDGA
                     SWLGDKIGDVWDYVQHPGKLVNKVMSGLNINFGGGANATVKIAKGAYSLLKKKLVDKV
                     KSWFEDFGGGGDGSYLFEYPIWQRFGRYTGGLNFNGGRHYGIDFGMPSGTNVYAVKGG
                     IADKVWTDYGGGNSIQIKTGANEWNWYMHLSKQLARQGQRIKAGQLIGKSGATGNFVR
                     GAHLHFQLMQGSHPGNDTAKDPEKWLKSLKGSGVRSGSGVNKAASAWAGDIRRAAKRM
                     GVNVTSGDVGNIISLIQHESGGNAGITQSSALRDINVLQGNPAKGLLQYIPQTFRHYA
                     VRGHNNIYSGYDQLLAFFNNRYWRSQFNPRGGWSPSGPRRYANGGLITKHQLAEVGEG
                     DKQEMVIPLTRRKRAIQLTEQVMRIIGMDGKPNNITVNNDTSTVEKLLKQIVMLSDKG
                     NKLTDALIQTVSSQDNNLGSNDAIRGLEKILSKQSGHRANANNYMGGLTN"
     gene            complement(10889..11047)
                     /locus_tag="O0293_000066"
     CDS             complement(10889..11047)
                     /locus_tag="O0293_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0278980.1"
                     /translation="MEQYGWTLTEVRKQPYVKLLEILNEENKEETEEKQSEQKVITGT
                     DLRKLFGS"
     gene            complement(11089..11439)
                     /locus_tag="O0293_000067"
     CDS             complement(11089..11439)
                     /locus_tag="O0293_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0278981.1"
                     /translation="MIKFEIKDRKTGKTESYTKEDVTMGEAEKCYEYLELVNQENKKE
                     APNATKMRQKERQLLVDLFKDEGLTEEDVLNKMSTKTYTKALQDIFREINGEDEEDSE
                     TEPEEMGKTEEQSQ"
     gene            complement(11497..11952)
                     /locus_tag="O0293_000068"
     CDS             complement(11497..11952)
                     /locus_tag="O0293_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HCY0278982.1"
                     /translation="MTKTLKVYKGDDVVASEQGEGKVSVTLSNLEADTTYPKGTYQVA
                     WEENGKESSKVDVPQFKTNPILVSGVSFTPETKSIMVNTDDNVEPNIAPSTATNKILK
                     YTSEHPEFVTVDENTGAIHGVAEGTSVITAMSTDGSDKSGQISVTVTNG"
     gene            complement(12044..12685)
                     /locus_tag="O0293_000069"
     CDS             complement(12044..12685)
                     /locus_tag="O0293_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCY0278983.1"
                     /translation="MAEGQGSYKVGFKRLYVGVFNPEATKVVKRMTWEDEKGGTVDLN
                     ITGLAPDLVDMFASNKRVWMKKQGTNEVKSDMSIFNIPSDDLNTVIGRTKDKNGTSWV
                     GENTRAPYVTVIGESEDGLTGQPVYVALLKGTFSLDSIEFKTRGEKAEAPEPTKLTGD
                     WMNRKVDVDGTSQGIVYGYHEGKEGEAEFFKKVFVGYTDSEDHSEDSAGSLPS"
     gene            complement(12720..13115)
                     /locus_tag="O0293_000070"
     CDS             complement(12720..13115)
                     /locus_tag="O0293_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HCY0278984.1"
                     /translation="MNILNTIKGILLSDAELKTHINSRIYYYKVTENAETSKPFVVIT
                     PVYDLPSDFMSDKYLSEEYLIQIDVESSNNQKTIDITKRIRYLLYQQNLIQASSQLDA
                     YFEETKRYVMSRRYQGIPKNIYYKNQRIE"
     gene            complement(13116..13517)
                     /locus_tag="O0293_000071"
     CDS             complement(13116..13517)
                     /locus_tag="O0293_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0278985.1"
                     /translation="MSVEIKGIPEVLNKLESVYGKQAMQAKSDKALNEASEFFIKALK
                     KEFESFKDTGASIEEMTKSKPYTKVGSQERAVLIEWVGPMNRKNIIHLNEHGYTRDGK
                     KYTPRGFGVIAKTLAASERKYREIIKKELAR"
     gene            complement(13514..13846)
                     /locus_tag="O0293_000072"
     CDS             complement(13514..13846)
                     /locus_tag="O0293_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0278986.1"
                     /translation="MEFNEFKDRAYFFQYINKGPYPDEEEKMKLYSCFCKIYNPSMKD
                     REILKATESKSGLTIIVRSSKTEYLPQTNHLVKIDSALYSDKLFNIVEIRIDTPDIGY
                     NTVVLSEK"
     gene            complement(13858..14136)
                     /locus_tag="O0293_000073"
     CDS             complement(13858..14136)
                     /locus_tag="O0293_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCY0278987.1"
                     /translation="MSLEEIKLWLRIDYNFENDLIEGLIQSAKSELLLSGVPDYDKDD
                     LEYPLFCTAIKYIIARDYESRGYSNDQSRSKVFNEKGLQKMILKLKKW"
     gene            complement(14205..15368)
                     /locus_tag="O0293_000074"
     CDS             complement(14205..15368)
                     /locus_tag="O0293_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCY0278988.1"
                     /translation="MPTLYELKQSLGMIGQQLKNKNDELSQKATDPNIDMEDIKQLET
                     EKAGLQQRFNIVERQVQDIEEKEKAKVKDKGEAYQSLSDNEKMVKAKAEFYRHAILPN
                     EFEKPSMEAQRLLHALPTGNDSGGDKLLPKTLSKEIVSEPFAKNQLREKARLTNIKGL
                     EIPRVSYTLDDDDFITDVETAKELKAKGDTVKFTTNKFKVFAAISDTVIHGSDVDLVN
                     WVENALQSGLAAKERKDALAVSPKSGLEHMSFYNGSVKEVEGADMYDAIINALADLHE
                     DYRDNATIYMRYADYVKIISVLSNGTTNFFDTPAEKVFGKPVVFTDAAVKPIVGDFNY
                     FGINYDGTTYDTDKDVKKGEYLFVLTAWYDQQRTLDSAFRIAKAKENTGPLPS"
     gene            complement(15380..16153)
                     /locus_tag="O0293_000075"
     CDS             complement(15380..16153)
                     /locus_tag="O0293_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCY0278989.1"
                     /translation="MSMKAKYFQMKRKSKSKGEIFIYGDIVSDKWFESDVTATDFKNK
                     LDELGDISEIDVHINSSGGSVFEGHAIYNMLKMHPAKINIYVDALAASIASVIAMSGD
                     TIFMHKNSFLMIHNSWVMTVGNAEELRKTADLLEKTDAVSNSAYLDKAKDLDQEHLKQ
                     MLDAETWLTAEEALSFGLIDEILGANEIAASISKEQYKRFENVPEDLKKDVDKITKID
                     DVDTSELVETPKESMSLEEKEKREKIKRECEILKMTMNY"
     gene            complement(16137..17375)
                     /locus_tag="O0293_000076"
     CDS             complement(16137..17375)
                     /locus_tag="O0293_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCY0278990.1"
                     /translation="MNVIAKENIVTRIKKKLIDNWIDQSASKLYDFSPWKNKSFWGVI
                     NNTLETNETIFSAITKLSNSMASLPLKMYEDYKVVNTEVSDLLTVSPNNSLSSFDFIN
                     QIETIRNEKGNAYVLIERDIYHQPSKLFLLNPDVVEMLIENQSRELYYSIHAATGNKL
                     IVHNMDMLHFKHIVASNMVQGISPIDVLKNTTDFDNAVRTFNLTEMQKPDSFMLKYGS
                     NVGKEKRQQVLEDFKQYYEENGGILFQEPGVEIEPLPKKYVSEDIVASENLTRERVAN
                     VFQLPSVFLNARSNTNFAKNEELNRFYLQHTLLPIVKQYEEEFNRKLLTKTDREKNRY
                     FKFNVKSYLRADSATQAEVYFKAVRSGYYTINDIREWEDLPPVEGGDKPLISGDLYPI
                     DTPLELRKSLKGGDKNVNES"
     gene            complement(17380..19071)
                     /locus_tag="O0293_000077"
     CDS             complement(17380..19071)
                     /locus_tag="O0293_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCY0278991.1"
                     /translation="MTIKVLNEPSPKLLTTWYAEQVTQGKIKTSKYVKKECERHLRYL
                     ENGGKWVFDEELAHRPIRFIEKFCKPSKGSKRQLVLQPWQHFIIGSLFGWVHKETKLR
                     RFKEALIFMGRKNGKTTTISGVANYAVSQDGENGAEIHLLANVMKQARILFDESKAMI
                     KASPKLDKNFRTLRDEIHYDATISKIMPQASDSDKLDGLNTHMGIFDEIHEFKDYKLI
                     SVIKNSRAARLQPLLIYITTAGYQLDGPLVDMVEAGRDTLDQIIEDERTFYYLASLDD
                     DDDINDSSNWIKANPNLGVSINLDEMKEEWEKAKRTPAERGDFITKRFNIFANNDEMS
                     FIDYPTLQKNNEIVSLEELEGRPCTIGYDLSETEDFTAACATFALDNGKVAVLSHSWI
                     PKHKVEYSNEKIPYREWEEDGLLTVQDKPYIDYQDVLNWIIKMNEHYVVEKITYDRAN
                     AFKLNQELKNYGFETEETRQGALTLSPALKDLKEMFLDGKIIFNNNPLMKWYINNVQL
                     KLDRNGNWLPSKQSRYRKIDGFAAFLNTYTDIMNKVVSDKGEGNIEFISIKDIMR"
     gene            complement(19061..19366)
                     /locus_tag="O0293_000078"
     CDS             complement(19061..19366)
                     /locus_tag="O0293_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HCY0278992.1"
                     /translation="MKLTKKQLKEYIEDYKKSDDILINLYIETYEFYCRLRDELKNSD
                     LMIEHTNKAGASNIVKNPLSIELTKTVQTLNNLLKSMGLTAAQRKKIVQEEGGFGDY"
     gene            complement(19493..19807)
                     /locus_tag="O0293_000079"
     CDS             complement(19493..19807)
                     /locus_tag="O0293_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCY0278993.1"
                     /translation="MTKHNNIYKHGRKSYQYDWFYHSKAWKKLREIALDRDNHLCQMC
                     LREDIVTDANIVHHIIYVDEDFNKALELDNLMSVCYGCHNKIHANGNDKSNLKKIRVL
                     KI"
     gene            complement(19964..20401)
                     /locus_tag="O0293_000080"
     CDS             complement(19964..20401)
                     /locus_tag="O0293_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCY0278994.1"
                     /translation="MGNTIYDIKPGTFKYIESEIYNLNENKKEIKRLRLEILNPTKEQ
                     DSNIVYGPLQKGEPVRTTELMATRLLTNKMLRNLEEMVEAVESEYLKLPEDHKKVIRL
                     KYWNKEKKLKMEQIGHECHMHRNTVTTIRKNFVKAVAYHAGIK"
     gene            complement(20414..21781)
                     /locus_tag="O0293_000081"
     CDS             complement(20414..21781)
                     /locus_tag="O0293_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCY0278995.1"
                     /translation="MEHLAIDFKPHSYQKYAIDKVIDNEKYGLFLDMGLGKTVSTLTA
                     FSELQLLDTKKMLVIAPKQVAKDTWVDEVDKWNHLNHLKVSLVLGTPKERNDALNTEA
                     DIYVTNKENTKWLCDQYKKEWPFDMVVIDELSTFKSPKSQRFKSIKKKLPLINRFIGL
                     TGTPSPNSLQDLWAQVYLIDRGERLESSFSRYRERYFKPTHQVSEHIFKWELRDGSEE
                     KIYKQIEDICLSMKAKDYLDMPDRVDTKQTVVLSEKERKVYEELEKNYILESEEEGTV
                     VAQNGASLSQKLLQLSNGAVYTDEEDVRLIHDKKLDKLEEIIEESQGQPILLFYNFKH
                     DKERILQRFKEATTLDDSNYKERWNSGDIKLLIAHPASAGHGLNLQQGGHIIVWFGLT
                     WSLELYQQANARLYRQGQNHTTIIHHIMTDNTIDQRVYKALQNKELTQEELMKAIKAR
                     IAKHK"
     gene            complement(21762..22052)
                     /locus_tag="O0293_000082"
     CDS             complement(21762..22052)
                     /locus_tag="O0293_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HCY0278996.1"
                     /translation="MKESTLEKYLVKEISKLNGLCLKWVAPGTRGVPDRIIIMPEGKT
                     YFVEMKQEKGKLHPLQKYVHRQFENRDHKVYVLWNKEQVNTFIRMVGGTFGD"
     gene            22186..22284
                     /locus_tag="O0293_000083"
     CDS             22186..22284
                     /locus_tag="O0293_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0278997.1"
                     /translation="MNLMQHWKQTGLNLTARRERGVVSFVASMLHH"
     gene            complement(22393..24840)
                     /locus_tag="O0293_000084"
     CDS             complement(22393..24840)
                     /locus_tag="O0293_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated E family protein"
                     /protein_id="HCY0278998.1"
                     /translation="MLDKVTQIETIKYDRDVSYSYAASRLSTYWTNHNMAWSDFMQKL
                     AQTVRTKEDLTEYNKMSKSEQADIKDVGGFVGGYLKEGKRRAGQVMNRSMLTLDIDYA
                     AQDMTDILSMFYDFAYCLYSTHKHREISPRLRLVIPLKRNVNADEYEAIGRKVADIVG
                     MDYFDDTTYQPHRLMYWPSTSNDAEFFFTYEDLPLLDPDKILNEYVDWTDTLEWPTSS
                     KEESKTKRLADKQGDPEEKPGIVGAFCRAYTIEEAISTFIPDLYEKHSTNRYTYHEGS
                     TAGGLVLYENNKFAYSHHNTDPVSGMLVNSFDLVRIHLYGAQDEDAKTDTPVNRLPSY
                     KAMQQRAQNDEVVKKQLINDKMSDAMQDFDEIVNSDDAWSETLEITSKGTFKASIPNI
                     EIILRNDPNLKGKIAFNEFTKQIECLGKVPWNTNFKTRQWQDGDDSSLRSYIEKIYDI
                     HHSGKTKDAIISVAMQNAYHPVRDYLNKISWDGHKRLEKLFIKYLGVEDTEVNRTTTK
                     KALTAGIARVMEPGCKFDYMLTLYGPQGVGKSALLKKLGGAWFSDSLVSVTGKEAYEA
                     LQGVWLMEMAELAATRKAEVEAIKHFISKQVDRFRVAYGHYIEDFPRQCIFIGTTNKV
                     DFLRDETGGRRFWPMTVNPERVEVNWSKLTKEEIDQIWAEAKYYYEQGEELFLNPELE
                     EEMRSIQSKHTEESPYTGIIDEYLNTPIPSNWDDLTIFERRRFYQGDVDMLPTGNVDY
                     VERNKVCALEVFVECFGKDKGDSRGSMEIRKISNILRQLDNWSVYDGNKSGKIRFGKD
                     YGVQIAYVRDESLEDLI"
     gene            complement(25128..25328)
                     /locus_tag="O0293_000085"
     CDS             complement(25128..25328)
                     /locus_tag="O0293_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HCY0278999.1"
                     /translation="MWIVISIVLAIFLLILLSSISHKMKTIEALEYMNSYLFKQLVKN
                     NGVEGLEDYENEVERIRKRFKS"
     gene            complement(25396..25548)
                     /locus_tag="O0293_000086"
     CDS             complement(25396..25548)
                     /locus_tag="O0293_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCY0279000.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDYEKI
                     RAEVSW"
     gene            complement(25545..25931)
                     /locus_tag="O0293_000087"
     CDS             complement(25545..25931)
                     /locus_tag="O0293_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279001.1"
                     /translation="MIKKLKNMDGFDIFIVGILSLFGIFALLLVITLPIYTVASYQHK
                     ELHQGTITDKYNKRQDKEDKFYIVLDNKQVIENSDLLFKKKFDSADIQARLKVGDKVE
                     VKTIGYRIHFLNLYPVLYEVKKVDKQ"
     gene            complement(25924..26160)
                     /locus_tag="O0293_000088"
     CDS             complement(25924..26160)
                     /locus_tag="O0293_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279002.1"
                     /translation="MINIPKMKFPKKYTEIIKKYKNKAPEEKAKIEDDFIKEIKDKDS
                     EFYSPTMANMNEYELRAMLRMMPSLIDTGDDNDD"
     gene            complement(26153..26356)
                     /locus_tag="O0293_000089"
     CDS             complement(26153..26356)
                     /locus_tag="O0293_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279003.1"
                     /translation="MTEVKIKTISDRVYYTTTDLASDDYINLVMNLVIEDFLPVKDVF
                     NNEVWVKRDEIESFTFIKEANDD"
     gene            complement(26353..26559)
                     /locus_tag="O0293_000090"
     CDS             complement(26353..26559)
                     /locus_tag="O0293_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HCY0279004.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAERKY
                     EAQVKRDAVIKVGQLFENIRECGK"
     gene            complement(26596..27132)
                     /locus_tag="O0293_000091"
     CDS             complement(26596..27132)
                     /locus_tag="O0293_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTPase"
                     /protein_id="HCY0279005.1"
                     /translation="MTNTLTIDQLQELLQIQKEFDDRIPTLNLRDSKIAYVVEFFEWF
                     NTLETFKNWKKKPGKPLDVQLDELADMLAFGLSIANQVGVSSEEIKEAIESSFKNSEF
                     HNMFNFKDKEFAQDAVVSTPQIIFKEFYPDQLAIVIAIDIAFNLYSIDQLIDAYKKKM
                     KRNHERQDGTADAGKGYV"
     gene            complement(27125..27373)
                     /locus_tag="O0293_000092"
     CDS             complement(27125..27373)
                     /locus_tag="O0293_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HCY0279006.1"
                     /translation="MNNREQIEQSIISASAYNGNDTEGLLKEIEDVYKKAQAFDEILE
                     GLPNAMQDALKEDIGLDEAVGIMTGQVVYKYEEDQEND"
     gene            complement(27388..27630)
                     /locus_tag="O0293_000093"
     CDS             complement(27388..27630)
                     /locus_tag="O0293_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279007.1"
                     /translation="MTDSARKERLNQFFGSKRYLYQDNERVAHTHVVNGTYYFHGHIV
                     PGWQSVKKTFDTAEELEIYIKQHGLEYEEQKQLTLF"
     gene            complement(27645..27845)
                     /locus_tag="O0293_000094"
     CDS             complement(27645..27845)
                     /locus_tag="O0293_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279008.1"
                     /translation="MATQKQVDYVMSLQEQLELEDCEKYTDEQVKAMSHKEVSNVIEN
                     YKTSIRNEELYYECMSFGLPNC"
     gene            complement(27848..28105)
                     /locus_tag="O0293_000095"
     CDS             complement(27848..28105)
                     /locus_tag="O0293_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HCY0279009.1"
                     /translation="MSIISNRKVDMNETQDNVKQPAHYTYGDIEIIDFIEQVTAQYPP
                     QLAFAIGNAIKYLSRAPLKNGHEDLAKAKFYVDRVFDLWEG"
     gene            complement(28105..28506)
                     /locus_tag="O0293_000096"
     CDS             complement(28105..28506)
                     /locus_tag="O0293_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVL family protein"
                     /protein_id="HCY0279010.1"
                     /translation="MARRKVIRVRIKGKLMTLREVSEKYHISPELLRYRYKHKMRGDE
                     LLCGRKDSKSKDEVEYMKSQIKDEEKEREKIRKKAILNLYQRNVRAEYEEERKRRLRP
                     WLYDGTPQKHSRDPYWFDVTYNQMFKKWSEA"
     gene            complement(28506..28691)
                     /locus_tag="O0293_000097"
     CDS             complement(28506..28691)
                     /locus_tag="O0293_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3113 family protein"
                     /protein_id="HCY0279011.1"
                     /translation="MQHQAYINASVDIRIPTEVESVNYNQIDKEKENLADYLFNNPGE
                     LLKYNVINIKVLDLEVE"
     gene            complement(28704..30656)
                     /locus_tag="O0293_000098"
     CDS             complement(28704..30656)
                     /locus_tag="O0293_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HCY0279012.1"
                     /translation="MNIDIETYSSNDISKCGAYKYTEAEDFEILIIAYSIDGGAISAI
                     DMTKVDNEPFHADFETFKIALFDPAVKKYAFNANFERTCLAKHFNKQMPPEEWICTMV
                     NSMRIGLPASLDKVGEVLRLQNQKDKAGKNLIRYFSIPCKPTKVNGGRTRNLPEHDLE
                     KWQQFIDYCIRDVEVEMTIAHKIKDFPVTAIEQAYWVFDQHINDRGIKLSKSLMLGAN
                     VLDKQSKEELLNQAKHITGLENPNSPTQLLAWLKDDQGLDIPNLQKKTVQEYLKEATG
                     KAKKMLEIRLQMSKTSVKKYNKMHDMMCSDERVRGLFQFYGAGTGRWAGRGVQLQNLT
                     KHYISDTELEIARDLIKEQRFDDLDLLLNVHPQDLLSQLVRTTFTAEEGNELAVSDFS
                     AIEARVIAWYAKEQWRLDVFNTHGKIYEASASQMFNVPVESITKGDPLRQKGKVSELA
                     LGYQGGAGALKAMGALEMGIEENELQGLVDSWRNANPNIVNFWKACQEAAINTVKSRK
                     THHTHGVRFYMKKGFLMIELPSGRALAYPKASVGENSWGSQVVEFMGLDLNRKWSKLK
                     TYGGKLVENIVQATARDLLAISIARLEASGFKIVGHVHDEVIVEIPRGSNGLKEIETI
                     MNKPVDWAKGLNLNSDGFTSPFYMKD"
     gene            complement(30724..31281)
                     /locus_tag="O0293_000099"
     CDS             complement(30724..31281)
                     /locus_tag="O0293_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HCY0279013.1"
                     /translation="MKAKVLNKTKVITGKVRASYAHIFEPHSMQEGQESKYSISLIIP
                     KSDTSTIKAIEQAIEAAKEEGKVSKFGGKVPANLKLPLRDGDTEREDDVNYQDAYFIN
                     ASSKQAPGIIDQNKIRLTDSGTVVSGDYIRASINLFPFNTNGNKGIAVGLNNIQLVEK
                     GEPLGGASAAEDDFDELDTDDEDFL"
     gene            complement(31307..32473)
                     /locus_tag="O0293_000100"
     CDS             complement(31307..32473)
                     /locus_tag="O0293_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HCY0279014.1"
                     /translation="MKLDHSNRAHAKLSASGAKQWLNCPPSIKASEGIADKSTVFAEE
                     GTFAHELSELYFSLKYEGLTQFEFNKAFQNYKRNQYYSEELREYVEEYVANVEEKYNE
                     ALSRDNDVIALFETKLDLGKYVPESFGTGDVIIFSGGVLEIIDLKYGKGIEVSAIDNP
                     QLRLYGLGAYELLSLMYDIHTIRMTIIQPRIDNFSTEELPISRLLQWGADFVKPLARL
                     AYNGGGEFKAGSHCRFCKINHSCRTRAEYMQNVPQKPPHLLSDEEIAELLYKLPDIKK
                     WADEVEQYALNQAKENDKNYPGWKLVEGRSRRMITDTKAMLEKLVEAGYKPEDITETK
                     LLSITNLEKLIGKKAFSKITEGFIEKPQGKLTLATESDKRPAIKQSAEDDFDKL"
     gene            complement(32470..32832)
                     /locus_tag="O0293_000101"
     CDS             complement(32470..32832)
                     /locus_tag="O0293_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279015.1"
                     /translation="MAILEDIFEELKLLNNNLRVLNTELSTVDSSIVQEKVKEAPMPK
                     EETAQLESIEEVKETSADLTKDYVLSVGKEFLKKADTSDKKEFRNKLNELGADKLSTI
                     KEEHYEKIVDFMNARINA"
     gene            complement(32846..33106)
                     /locus_tag="O0293_000102"
     CDS             complement(32846..33106)
                     /locus_tag="O0293_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HCY0279016.1"
                     /translation="MYYKFGEIKNKIISFNGFEFKVSAMKKHDGISIQIKDMNNIPLK
                     SFHVVDLSELYFATDAMRDVINEWIEENTDEQDRLINLVMKW"
     gene            complement(33111..33413)
                     /locus_tag="O0293_000103"
     CDS             complement(33111..33413)
                     /locus_tag="O0293_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2482 family protein"
                     /protein_id="HCY0279017.1"
                     /translation="MTKKYKDMTQEEVKDLLSEKSGELYELAKEIKGESKFDILLFSS
                     IGVIDGDYLAGSSSVIGHTFDLASLLDSTKSYKDIVNVLQMCKSQKFHGIDDNKEG"
     gene            complement(33406..33723)
                     /locus_tag="O0293_000104"
     CDS             complement(33406..33723)
                     /locus_tag="O0293_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279018.1"
                     /translation="MAENIKTEQHYYTKDFSGYRNEEDNFVANQELTVTITLNEYRKL
                     IEIKAVKDKEEDTYRGKYFAEERKNEKLEKENIKLKNKIYELQNEEDNEEDEENEEEV
                     NND"
     gene            complement(33816..33977)
                     /locus_tag="O0293_000105"
                     /pseudo
     CDS             complement(33816..33977)
                     /locus_tag="O0293_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500084.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
     gene            complement(33989..34252)
                     /locus_tag="O0293_000106"
     CDS             complement(33989..34252)
                     /locus_tag="O0293_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCY0279019.1"
                     /translation="MPKIIVPPTPENTYRGEEKFVKKLYATPTQIHQLFGVCRSTVYN
                     WLKYYRKDNLGVENLYIDYSPTGTLINISKLEEYLIRKHKKWY"
     gene            complement(34277..34492)
                     /locus_tag="O0293_000107"
     CDS             complement(34277..34492)
                     /locus_tag="O0293_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279020.1"
                     /translation="MNKKSEGLDIIIPRIFRRDHASVESLTENERRLRKEILGSIKKG
                     DYSYLEINKVFYALDRELQYRANNNKL"
     gene            34539..34778
                     /locus_tag="O0293_000108"
     CDS             34539..34778
                     /locus_tag="O0293_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279021.1"
                     /translation="MISIHKRLLTQYLDKEIVTSLDLHLINGEVIKVQEHIKDAESKT
                     LHIIHPKDRVVSLDHVLYFDINVKGEKNNDSPYPS"
     gene            complement(34775..34951)
                     /locus_tag="O0293_000109"
     CDS             complement(34775..34951)
                     /locus_tag="O0293_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279022.1"
                     /translation="MNEEKLKMILLLLEDIPRDEWNRLVNEVNNQYSYQADKVGLASD
                     NCQQIANNYKHYGF"
     gene            complement(34967..35719)
                     /locus_tag="O0293_000110"
     CDS             complement(34967..35719)
                     /locus_tag="O0293_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HCY0279023.1"
                     /translation="MQALQTNSNIGEMFNIQEKENGEIAISGRELHQALEVKTAYKDW
                     FPRMLKYGFEENTDYTAIAQKRATAQGNMTHYIDHALTLDTAKEIAMIQRSEPGKRAR
                     QYFIQVEKAWNSPEMIMQRALKIANNTINQLETKIERDKPKIVFADAVATTKTSILVG
                     ELAKIIKQNGVNIGQRRLFEWLRQNGFLIKRKGVDYNMPTQYSMERELFEIKETSITH
                     SDGHTSISKTPKVTGKGQQYFVNKFLGEKQTS"
     gene            35770..36099
                     /locus_tag="O0293_000111"
     CDS             35770..36099
                     /locus_tag="O0293_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279024.1"
                     /translation="MTNEAKFVLLQLYSIYLDRIDEGMSKRSASYFGSDESSFNAFFL
                     DFNFEDYIDAVLELKHRDFVIASAEDGGFLEMALSREGIAYSESESKKDYKTLMDLIR
                     DLKKLII"
     gene            complement(36088..36303)
                     /locus_tag="O0293_000112"
     CDS             complement(36088..36303)
                     /locus_tag="O0293_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HCY0279025.1"
                     /translation="MNIQEATKLAMEKGISIRRENQDVYGILPTNLQRYQCLVVSRHY
                     KKKRQTAAGRWQPSADDLIADDWILDY"
     gene            complement(36319..36582)
                     /locus_tag="O0293_000113"
     CDS             complement(36319..36582)
                     /locus_tag="O0293_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0279026.1"
                     /translation="MKTLKELRTDYGLTQKELGDLFKVSSRTIQNMEKDSTNIKDSLL
                     SKYMSAFNVKYDDIFLGNEYENFVFTNDKKKSIILAFKEKQTS"
     gene            complement(36579..36752)
                     /locus_tag="O0293_000114"
     CDS             complement(36579..36752)
                     /locus_tag="O0293_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCY0279027.1"
                     /translation="MFLAIIIFLSLFILLTSFFLIIRNFHIIVKFFTKKTYLMLTIRK
                     FRIILGLRKAVTT"
     gene            36715..37428
                     /locus_tag="O0293_000115"
     CDS             36715..37428
                     /locus_tag="O0293_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0279028.1"
                     /translation="MNKERNIIIAKNIRKFLNDSNMSQKKLAELINIKPSTLSDYLNL
                     RSNPSHGVIQRIADVFEVGKSDIDTTYKDDNDITSIYNKLTPPRQENVLNYANEQLEE
                     QNSKGDNVVDINSYKQEKTPVNVNGCVSAGVGERLHDETLFTEMVKGPIPTHDLALKV
                     NGDSMEPMFKDGEIIFVEKTHNIKNGQIGIFIIEEEAYVKKVFVEDDRLTLVSLNKDY
                     DDLHFYRNESVRLIGKVIL"
     gene            37444..38376
                     /locus_tag="O0293_000116"
     CDS             37444..38376
                     /locus_tag="O0293_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCY0279029.1"
                     /translation="MKKYDIAVLDFETMNEHMNSPCEVAVSLIKDLSIVKVYSSYINP
                     PNNRYNLKNAKIHKIPEDVILKAPKYPDIYQEILYLLKESHLIIAHNALFDISVLKNT
                     NNYYDLPVPNFMYVDSINIFRSFHAISSFKLENLCSLYDIDKEKLHSAKFDVLALSKM
                     LISLAKNNQHYSVLKLIHYMPKQYIRFSKYSNSPTKLFDSGFQKIHMKISEINKIEVE
                     SVIPILKDKNVVFTGNFDTEKQDLMILTRKKGAYIRSDVTAKTDILVEGVQDDKYKDV
                     NGLVSKQRKAREYVGNGAKIQFLNEEDLINLIKE"
     gene            38382..38723
                     /locus_tag="O0293_000117"
     CDS             38382..38723
                     /locus_tag="O0293_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279030.1"
                     /translation="MIKKIFTKKHVFLVIEDENHNHSDAVFGKSILLSIYVGVNKKTN
                     SKSGKFIYLDRSKRIVRQSDITKIESANENDVDFYNLLKKEKEIVYSKNIVDKYNLAN
                     YIIYYEVSTKE"
     gene            38927..39109
                     /locus_tag="O0293_000118"
     CDS             38927..39109
                     /locus_tag="O0293_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279031.1"
                     /translation="MKITNCKIKRETVIYEVLTSGNQPFTYELPKDLSSHNARKYLEF
                     ISQKIDGDKLTKEDSL"
     gene            39187..>39851
                     /locus_tag="O0293_000119"
     CDS             39187..>39851
                     /locus_tag="O0293_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCY0279032.1"
                     /translation="MTHNIEKRINKLKTSGNPKFKKLDSDIHYLLKRFEGEKNHKGFY
                     PKFKQGEIVFVDFGINVNKEFSNSHFAIVMNKNDSNTEDIVNVIPLSSKENKKYLKMN
                     FDLKWEYYLRLFLNLISAQNNSAILKEVFDKKYQKNNTEFITKDYFSEFISDSLEIEN
                     KLNKIDRNINNIVSAIDKVKKLKGNSYACINSFQPISKFRIRKVLPQKIKNPVIDSSD
                     IMLL"
BASE COUNT        11488 a         7735 c         5505 g        15123 t
ORIGIN      
        1 tgttttctaa atttatagct acagatttct ctaaattact cattaactta taattctccc
       61 ttcgtgtaaa gtccatggcc ctgaacttgt tttactatca taatttttca atagtatctc
      121 agcagatgct gtaacactat tacgaactag cctatgaaca aagccacctg tgtttgaagc
      181 ttctacatat aagttccaac cagctacccc tttacgttca gttggaaaat ctgtaaaacg
      241 ttttgtatca tccgtagtta aataaaacga catgcctact atgttaatat ctgacatttt
      301 tgtgatgaat gaaggtactc tctcccattt accactattt ttaggcacat aattccagtc
      361 cgaaatgtct ccagttcttc cagaaagcac cctttcaaaa gtcatcatat tccttgcata
      421 actattacgc gtcaatatct gaattacatc accgccagtt tgtggtggct taacttccaa
      481 gaaccaacct gcatcacgcc attctcttgg taatgggaaa tcatcgattt gaactgtatg
      541 atcagtgtat aaatagtaaa gacctggctc tgttaacatc ccaagattct taagtttatc
      601 aggcctcatt ggtaaaggtt taactctacc acctgtgtca ctcatgataa aaggaacgcc
      661 tcttgagtga agtatttcta aaatacctct ttgcccaatc atgaaaatac gatgtgttct
      721 atttccatca ccaccgacag taacacctag catcaaagct tttttaccac tatctttgtc
      781 atagtatatt tgcaaacctt ctgcttccgc aaattcgcca ggaaatgaat ctagtgttcc
      841 accatagtca gcattaacct gatacgcttc ttctcctgtt tctaaatcga aagccgttaa
      901 atagtttcta ttatttggat tactgtctcc tgtataccaa tacaagtatt tttcatcaaa
      961 agtcacaccc tgcattggtt gggtttcgtt tgttagtctc atagggatac tgattttatg
     1021 caaaacttta tcaatatttt tatcaacatc gtctaaactt cttatctcta tataattcat
     1081 tgagttttca agttcccact gacttctagg tctctcaatt ctgtatagaa ttttattttc
     1141 tttttcattt atgacagggg tgatgtaggg tttttctggg tgtcctgtaa atacatcttg
     1201 cataccatac ttgccatagc taatttccac attaggcgta tacttgaaac gaactaatgt
     1261 attctcatta ttaccattta agataaaact ataaatccat aactcatcat caatatatct
     1321 ataaccgtta tgtgtaccat gacccccacc tacaatcaat gagctgtcta taaattgacc
     1381 attaggtctt aaacgactta gcatatagcc attatttcta gcttgtgtca tgtatactat
     1441 gcctgttcta ttatcaaacc agaaggattg cattactgca tttgtaagag gtgcaagttc
     1501 tgtcacaaat aaaaactctt gcttatcagg ttcaaaacga tactcgatat caagaatttc
     1561 ttgtttggtc ttatttaatt ctcttatagt ttcctcttta ttaatttgag ttttggtttc
     1621 ccaatcgtct aaatgttctt ttaatgtgtc aaaggtttcg ccgtttacat taactcgagc
     1681 ttgaacaatc tcattagcac tgttattacg tggtgccaca acaagtgcgt taatttgact
     1741 ttgtaaagat ttgtttactg ctgcttgcga tctaccatta taataaattt gctcagcgaa
     1801 gtgttgcatt gttttagctt tctgatgcaa cttaaactct gttgtcaagc caagcgcaaa
     1861 ttgctctatt ctttgcaggt tttgaatttc tttagctcta taatctcgac ctgctaaagc
     1921 tcccaaatcc tttattaaat acaaattttc cataatgcac cttcctttct aataaaatag
     1981 cactgtacca agtttcccac tatcgtcaac tgttattttc cacaatttac cgtttgggga
     2041 tttctgtaca atgctatttt gaataattcc tgcttcgcct atttttaatt tatctaattt
     2101 atttttatca tctaccgaaa tgataccgtc ttgaggcaat ccatcaatat cactactgcc
     2161 tgcataaggt atcccattta tagctttcca gtgtgtagct ggaaagtact gtttatcgtt
     2221 ttcaagtagc gctttgattt taacttcttc tgttgccatt atattaatac actccctata
     2281 tccattgtct cgaaaggaga attcaaagta ctagtgtata aatgatttat acgatttgct
     2341 tgatagttat atctattatc ttgtgcaata actcgtctgt taagtgcttg ttgaatttgt
     2401 accatatctt ttatttcatt gctgaaagac acttcatcta ttgcgtttac aaatggatgt
     2461 gacctatcaa gtttaacaac ctttaattca gtgttatatc ccattaattc atgaacaaaa
     2521 aatacgctat ctcttggctc tattttttca taacctatat aattaacatc taattcagtc
     2581 ttaggagtat catttatttg cttttttgca aattctaaca gcttatcctg tgtttcgata
     2641 tcttcatttg tttgcgtatt agcatatcga atcccaaact gctttgcact atctgcgacg
     2701 tagtcgacaa ttgctttgta ttgattgcga cctgaattat cagcaattaa atttaagact
     2761 gttgattttt cagttccaac atacatacaa ggcttagctt ttttatttga agatatatca
     2821 attctatttt tggggtcttc tcctaaaaat atcatttcta aaacgtgctt gcctttatca
     2881 atatttttta ttaaatctat tgtttcagac tgaaccgact tagcaaaaca agaaatttgc
     2941 ttaatttgct tgccgtctaa aatcaactta tatattccac cttgagagcc cttttttatt
     3001 gtaaatctaa ctgtttcatt accatacttg caatcaaagt taatagtagc tttagaccca
     3061 attgtttcgg tacgataagt accttctttt ataaaaccat ttgaatattt aatgtcagtt
     3121 gttctaatag gattataatt tttcttttcc tcagctgtat acttttttcc aaaaactttt
     3181 atagctgttc ttaattccaa tgtactgaca gttgcagata cagtatcagt attatattga
     3241 tatcgaatca ctttctcgct tctttgataa aatgtttcag gagaataaaa accaatctct
     3301 gtatcatttg ggtaaattat acagccaaac aggtctaccg cttctttaca gtattctaag
     3361 ccgtttttgt tacctaattc gtcaatcggt acttttcgct taaaatctcc aattatttta
     3421 taggtcattt tgaccgaagt tttttgattt gcaaatccat atcttaagta ctcatctaaa
     3481 gagtattctg gcgttttacc agtttcgcta ctgtcgtcat caagcttatt tgattccact
     3541 gagtgatttt gaaattcata cattatgtga tatgccgtaa cttcaataaa aactttatca
     3601 ccttcaacct ttggcgctgt ctgcttaatt gtatattttt caccatgata aattatgaag
     3661 ttttcacaaa tcaataaatc aaaaacaaaa ctattatgag tagttctata aactgtaaag
     3721 gtgatgtacc tagcttcatt cagttcataa tattctttaa aagaaccata atctacatct
     3781 agtaaatttt cacaaatcaa ttcattaaaa tccattactg ataaatgatc atgataatcc
     3841 attaaatcac ctacctataa ataaaaggaa acttaaatgt agttttaata tcactgacgt
     3901 ctcctttaat cttaaattca tttttacctg gcgctaatgt tataatgcct ctatttgtat
     3961 caattcccac tctatttata tctcgatatg catacacacc atctaaaaca aaatcagtgt
     4021 ttttatctat acttttgttg tacttaaaaa tatcacctgt tgtatagtta accagttcaa
     4081 atcctccact cgcatttaaa ttaattaata ttttcaaatc gtgcttgaat cgtggattta
     4141 tcgtatcagt agaaccgttc caaatagtaa attgatttga tgtatgagta tatttaggtg
     4201 tgaaatcaag aggaattcca ttttcaaaca tccaattaga gtcgaataag aactcgctat
     4261 cggtccaatt aactgattca gaataccctt tataaacatt taaacttact tcaatttcag
     4321 ttgaagaacc atcttttaaa ttagatgtaa cattagctgt attcactgca tatttaacac
     4381 caggcatttg agaagtaata acataataag gatgtctgcg attaaacaca gatctaaacc
     4441 aatgctcaaa taaatttaaa tctataacat ctataccatc atagccaaac cttaatacta
     4501 atgaaaaagg cgcaaaacta attgcgcccg gtaaaatacc atctactccg ttaatagtta
     4561 cactgttatc attggtgttt ggactttcag cccttgcatc taaaaatata agctgattaa
     4621 aatctgttat tacttcttcc ttgtaaccat ctatgatttt tacaaaagat tgcattaatt
     4681 agtcaaacct cccatataat tatttgcatt tgctctatgc ccactttgtt ttgacaatat
     4741 tttttctaaa cctctaattg catcattaga acctaagtta ttatcctgag aagaaacagt
     4801 ttgaatcaat gcatctgtta atttatttcc tttatcactt aacataacaa tttgtttcaa
     4861 caatttttca actgttgaag tatcattatt tacagtgatg ttatttggct tgccatccat
     4921 accgatgatg cgcataacct gttcagttaa ttgaattgct cgtttacgtc tagttaaagg
     4981 gataaccatc tcctgtttat ctccttcacc cacttcagca agttgatgct ttgtaatcaa
     5041 accaccattc gcatatcttc ttggaccact tggagaccaa ccacctcttg ggttaaactg
     5101 tgagcgccaa tatctgttgt taaagaacgc taataactga tcgtaaccac tatatatatt
     5161 gttgtgacct ctaacagcat aatgtctaaa tgtttgtggg atatattgaa gcaatccttt
     5221 tgctggattg ccctgtaaaa cgttgatgtc tctaagcgca ctagattgag ttatacctgc
     5281 atttcctcct gattcgtgtt gaatcaagct aataatattt cctacatcac ccgaagtaac
     5341 attaacaccc attcgttttg ctgcacgacg tatatcgcct gcccaagcag atgcagcctt
     5401 attaacacct gaaccacttc gaacgccact acctttaagt gacttcaacc atttttctgg
     5461 atctttagct gtatcattcc ctggatgtga cccttgcatc aattggaaat gtaagtgtgc
     5521 tcctctaacg aaattacctg tagcacctga tttccctatc agttgaccag ctttaatacg
     5581 ttggccttgt cttgctaatt gcttagataa atgcatatac cagttccatt cattagcacc
     5641 agtcttaatt tgtatagaat taccgccacc gtaatcagtc cataccttat ctgctatacc
     5701 acctttaacg gcataaacgt ttgttccaga aggcatacca aagtctatac catagtgacg
     5761 accgccatta aagttaagtc cacctgtgta gcgtccaaat ctttgccaga ttggatattc
     5821 aaatagatag cttccatcgc ctccaccacc gaaatcttca aaccacgatt ttactttgtc
     5881 tactaatttc tttttgagca atgagtatgc gcctttagct atttttactg tagcgttagc
     5941 tccgcctcca aaattaatat ttaaacctga cattacttta tttactagtt tccctggatg
     6001 ttgtacgtaa tcccacacat cgccgatttt atcgcctaac caagatgcac catctttgat
     6061 tttatcgcct gctgcttcaa ccatttcttc tgcacctttt ttgatattat gcgctgtgtt
     6121 tttagctgta gctccaaatt ctcctgcttt tttacctata ttacctttaa gttggtctag
     6181 ccaatctttc tttttcgtac ctccatgaaa ctttggtaaa acacccatac gctgtaactt
     6241 cagagtgtca ttagcattta ttacgctatc cccaactcct agtggaacaa ccacatctcg
     6301 tccttggggt gcatggaatg ttccgtcagc cctgtgaatt acttcttgaa ctccaccacc
     6361 tggggcgttt ccagaacctc tatcatttaa tacagcaaat gtcggttgcg ttaatgctcc
     6421 cgaattatcg gtagctacac cctttcctgc taaagtacca gtagacaatg taggtattgg
     6481 cttgatgaga tttttatcag taatggcttt agatatttta ttaataccgc caatcatgct
     6541 attcaaaccg ccaatagctt tattagcaac atttttacct aaatcagccg cagctcttcc
     6601 catgtcttta ccaatatctc taatccaatc atatgttttg gatagccatt ttctaaaacc
     6661 attaaatact gatttagcgt tagaccatgc cgaacttgaa attgcatcaa aacgatcgtg
     6721 ggctcttgaa tacatatccc cagtccaatc ttttaaagat ttgtatgagt tactaaacca
     6781 tttcgatgtt cctttccaaa cggattttgc attcgaccaa gctgtactag aaatattatc
     6841 ccatttcgcg cgagatttat tagccatatc cgttagccag ccctttgcac ttttatatgc
     6901 attgctaaac cattttgatg tgcctctcca aatagatttt gaatgcgccc aagctttatc
     6961 tgaagcatct gaatactttt gcttagtttg attgtaaata cttcctgttg tcgatttaac
     7021 agattgccaa gcttttccaa accatttacc agtactatta gctatagcct tagtgtggta
     7081 tcctacagaa cttttggctg agctccaacc tgaacttaat ttgcttggaa tccctttgat
     7141 tccgctccac atttttttca tttcgccgcc aaaatgatta gcatttctgc ccattttact
     7201 aaaggcttcg ccagttttac tttttacgcc gtcccaagca tttccaaacc atttctttat
     7261 attttctctg tttctacgag ctgtttcttc ttgttcttta gcgtatttat cgcttttttt
     7321 cttttggtct tctctaaagt tagaccacca acttttaagg ccattccacc acttttcagt
     7381 atttttatat acacgtccac tggataaatc catttcttta tcaatatctt tattttgctt
     7441 tttaacaaca tctactacag catctttttt agattttgct tttcttactt catctttatg
     7501 tctttgatca gcaatagcta acaatttatc tttttcagac ttagaaaggt tgacgttatt
     7561 ttttatagca atgacatcat cttcatattg cttgtccact tctttttttc ttgcttttct
     7621 tgctttttct gcttctttaa ttgctttgct cgcttcgtct attgaataag catttctgtt
     7681 tctttgcatt cttactaaaa tacgctcttg ctctttttca gtcttactca attctttaac
     7741 agtgatatca cgtctttgat tttcaagctt ttcaatttct tttctttcat tttctgaaat
     7801 ctgaccatca ctcaaagctt tttctttcaa ttctttgatt ttctgattga gttcttgctc
     7861 ttttttaatt cgcaagtcat ttttttcttt agttcgagtt aaaatgtttt gcttttcttg
     7921 ttcatcgaat gcactatact tatcaataag ttcttgagtt ttttcgagtt cctttttatt
     7981 tcttttttct atttcagcta taaggttatt agataaatcc gtttcaattt tcaaaagttt
     8041 ttttgctttg tcttctgata tctgacccga gtttaaacgt actttttcca tgattctgct
     8101 attttcttca gaataatgca catatttttc taaagctttt tctgtttctt ttgaaacacc
     8161 tttccctaac acttttacag tatcagacgc ttttttagaa gctgtgccca tggtttgcat
     8221 aaatccttta aacttgttga ctcctacttt gagaaggtca tcgtcgctta atgatttata
     8281 accatctttc atatcttttg aaaacttttc tttgaagctt ttgccgatac ttccaagata
     8341 gtttttaaac tctcctagct ttctaacagc gccgccaata attttaccac caaaaaactt
     8401 tatagtttct cctaaaccgt taataccgtt tctgaaccat tccacacgat catatgcggt
     8461 tttaaaaact ttatacgcaa ttgtaatagc agttattgta gcacctatag gtcctgttaa
     8521 aaaccttaag gctacacccg caaatcttgc gcctccactt actgcaaata aagattttgc
     8581 agctaatcct aaaccgtttt tcaaaagttt gaacggtaaa attgctagct ttgcagaatt
     8641 tttcaaaaca tttataggtt ttaaattaaa catcatagct ccggctaacc ctttaaagcc
     8701 ttttgacgtt tttcctgttg tagaaccaag aaataatgtt tgaagaccta aagatttcat
     8761 tgcttttgaa ttagtatttg aaaggattgt attttcagca atacgtctat ttaatgacgc
     8821 atatccttta gcagcacttc caactgtacg tattaataac cctccagcaa gaactgcagg
     8881 tccaattgct gcaccaaaaa gtgctaatcc tactgaagct tttctaaccc aaccagggag
     8941 atgtgtaaat ccatcaacta attttgttaa accttccgct cctgctctaa tcataggcgt
     9001 taaatcttta ccgacttcga ttgctaatga ttcaaaagcg ccacctaatt gttccagagc
     9061 gcctttgaga ttatctttca tcaaatctgc tgctttttta ctttcgccat tggaattctt
     9121 taaggattta ctatagctat taattttatc tggtcccgct tcaatcaagg ctaaaaatcc
     9181 acttgctgct tcagtaccaa ctattgtagc cactgtagct agtttttgtt ctctcgtcat
     9241 gcctttcata ttatcttgga actgtctaat caattcaccc atgccaacaa attgaccttt
     9301 agcatcagac aaatgaatac ctaatttttt catttcctta gctgtatttt tacttggatt
     9361 agctagcctg ataaatgaag ctcttagggc agtacctgct tgagaaccct ctaaacctga
     9421 gttagataaa acttcaattg ctgcggaagt gtcctctatt gaaactccta atgcttttgc
     9481 aggagtacca gcatacttca atgcatctcc catgtactga atatctgcag cactatcatt
     9541 tgctgatctc gcaagtaaat cagcaacatg atttgcatca gatgctttta aaccgaaaga
     9601 gttaatcgct gaagccatta cagttgcagt tgtagccatt tctgcaccac ttgcttctgc
     9661 tgcactgata acacctggca tagcctccat tgtttgtttg gcattaaagc ctaaagctgc
     9721 caattcttcc atacctttag caacttcgtt agcactttta ctggttttag ctcctaagtc
     9781 aactgcttga ttagacatgc ttttcaagtc tttactgctt gcttgcgcaa tcgctccaac
     9841 tcgagacatt tggccttcaa agtctgcact tgtttttaat gctgcaccta accctaaagt
     9901 aattggtgta gatacgccca tcgtcattgt acgtcccagg gaagtcattt tgtctccaat
     9961 agaactaaat ttctttgaca tgacatccgc ttgacttgca agtttaccga aatgactttg
    10021 agctatcatt tgttctttgt taaaagtctt catttcggat gaagctttat ctattgaacg
    10081 ctccaaatta tttaaagcag ctttttcttt attaacagct gtttcagctt ttgcgacatt
    10141 agcgctatga ttcttaatag tattgtttaa atcattaaat tctttttctg tttgctttaa
    10201 tttagtatta gttttagcgt aagaactttc aattttatca tttgattttg aaagattgtc
    10261 attttgcact tttagttttt gaacttgatt gccttcttgt ttatattgtt caacaagtgc
    10321 tttatgctta gcggactgct tctgtactgc gtcacttgct ctttttagtt gtgcagtagt
    10381 agcttggtta ctattcttaa gcttttgttc tgcatctctc aactgtttaa gtttttgata
    10441 cgcatcttgt ttacgttgat ttgtacgttt atattgattt tcagcttttt taagttctgt
    10501 attcgatgat tttaaggctt ctttagattt atcaagagct aatttttctt ttttattggc
    10561 ttctactaac tttaaatatg ctttctcaac atcttttaca ctggatttag ctttttggta
    10621 attagcgtta acttgtttaa gctcatcttc tacttgagaa tacatctttt tttgaacttt
    10681 aagcctatca tttaacccct taattctcgc ctgatatttt tccattgatt tttcagactt
    10741 atcaaatgct gacagattag ctttcatttc actattaaca acacctaatt gtcgctttaa
    10801 acctttcatg cattcttgga cacctaaatg gtctaatttc agctccaagg tcatgccttc
    10861 tactttttca ttcatattaa cctcctttct agcttccaaa aagttttctt aaatccgtac
    10921 ctgtaatgac tttttgttca ctttgttttt cttcagtctc ttctttattc tcttcattaa
    10981 gtatttctaa aagttttaca tacggctgtt ttctgacttc agttaatgtc cacccatact
    11041 gctccataca gaaacgttgt attttcttaa tgttcgataa aatgtctttt attgagattg
    11101 ttcttctgtc tttcccatct cttctggttc agtttctgaa tcttcttcat cttcaccatt
    11161 gatttctcga aatatatctt gtaaggcttt tgtataagtt ttagtactca tcttgttcag
    11221 aacatcttct tcagtcaatc cttcatcttt aaataaatct actaataact gtcgctcttt
    11281 ttgtctcatt tttgttgcgt taggtgcttc ttttttattc tcttgattta ctaattctaa
    11341 atactcatag catttttctg cttcgcccat tgttacatct tcttttgtat agctctctgt
    11401 ttttcctgtt ttacgatctt taatttcaaa tttaatcatt gtattagctc cttttattca
    11461 aataaaaaag acgcagatat actgcgcctt aaatccctat ccgtttgtta ctgtcactga
    11521 aatttgtcct gacttatcgc ttccatcagt agacatagca gtgattactg aagtaccttc
    11581 agctacaccg tgaattgctc ctgtattttc atctacagta acaaattctg gatgttcact
    11641 tgtatatttc aatattttat tcgttgctgt gcttggtgca atgtttggct caacattgtc
    11701 atcggtattt accataattg atttagtttc tggtgtaaat gatacgcctg agactagaat
    11761 tggattggtt ttgaattgag gtacatcaac tttactagat tctttaccat tttcttccca
    11821 tgccacttgg taagtacctt ttggataagt tgtatccgct tctaaattag ataaagttac
    11881 tgacactttg ccttcacctt gttcagaagc tacgacgtcg tctcctttat aaacctttaa
    11941 agttttagtc ataaattatt ctcctttgat ttattttgaa agcccctatt ctgctgaaac
    12001 tgttgcagat tttgaattaa ctgctacttc aacattttgg ggattagctg ggtaacgaac
    12061 ctgcagaatc ctctgaatga tcttcactgt ccgtgtatcc aacgaatact tttttgaaga
    12121 attctgcttc tccttcttta ccttcatgat aaccgtatac aataccttgt gacgttccat
    12181 caacatcaac ttttctattc atccagtcac ctgttaattt tgtaggttct ggggcttctg
    12241 ctttttcacc tcgtgtttta aattcaattg aatctaaact aaaagtacct ttaagtaagg
    12301 ctacatatac cggctgacct gttaaaccat cttccgattc gccaattact gttacatacg
    12361 gtgctcttgt attctctcct acccaagatg taccattttt atctttagta cgtccaataa
    12421 ctgtgtttaa atcatcactt ggaatattga aaatactcat gtcagactta acttcattag
    12481 taccttgttt tttcatccat acacgtttgt tagatgcaaa catatctact aaatctggtg
    12541 ctaaacctgt gatatttagg tcaactgtac cacctttttc atcttcccat gtcatgcgtt
    12601 taactacttt tgttgcttct gggttaaaaa ctccaacgta taatctttta aaacctactt
    12661 tataagaacc ttgtccttct gccattgctt atttcctcct taaaaattaa aaagcacacc
    12721 tattcgatgc gctgattttt ataatatata tttttgggta tgccttgata tcgtctcgac
    12781 atcacataac gtttagtttc ttcaaaataa gcatctaact gactagatgc ttgaattaaa
    12841 ttttgttgat ataacaggta tcttattcgt tttgttatat caattgtttt ctgattattt
    12901 gaagattcta catctatttg aattaagtat tcttcactga gatatttatc agacataaag
    12961 tctgaaggca aatcataaac aggtgtaata acaacaaagg gtttggaagt ttcagcgttt
    13021 tcagtgactt tgtaatagta tattctagaa tttatatgtg ttttgagctc tgcatcagat
    13081 aataaaattc cttttatggt gtttaatata ttcatttatc tggccaactc cttttttata
    13141 atttctctat acttacgttc gctagcagct aatgtttttg caataactcc aaaacctctt
    13201 ggtgtatatt tttttccatc tcttgtataa ccatgttcat tcaagtgaat aatgtttttg
    13261 cgattcatag ggcctaccca ttcaattaaa acagcccttt cttgactgcc aacttttgta
    13321 taaggcttag atttagtcat ttcttctata ctggcacccg tatctttaaa gctctcgaac
    13381 tctttcttta aagcctttat aaaaaattca gatgcttcat ttaaagcttt atcactctta
    13441 gcctgcattg cttgtttacc gtataccgat tctaatttat tcaacacttc aggtatccct
    13501 ttaatttcta cactcatttt tctgataaaa ccactgtatt atagccaata tctggtgtat
    13561 caattcttat ttctacaatg ttgaataatt tatcggaata taatgcactg tcaattttaa
    13621 ctaagtgatt tgtttgtggt agatattcag ttttagaaga cctgacaatt atggttaatc
    13681 ctgattttga ttcagtcgct tttaaaattt ctctatcttt catagaagga ttataaattt
    13741 tacaaaagca actatacaat ttcatttttt cctcttcatc tggatatggt cctttgttta
    13801 tatattgaaa aaaatacgcg cgatctttaa attcattaaa ttccatttaa aaatcaccta
    13861 ccactttttt aatttcaaaa tcattttttg caatcctttt tcattaaaca ccttgcttct
    13921 agattggtca tttgagtatc cacgactttc ataatctctt gcaatgatat atttaatcgc
    13981 tgtacaaaaa agcgggtatt ccaagtcatc tttgtcataa tctggaaccc cacttaatag
    14041 taattcagac ttagccgatt gaatgagacc ttcaattaaa tcattttcga aattatagtc
    14101 aattctcaac cacaatttaa tttcttctaa actcatttca tcacccctat tcggctgata
    14161 ttacagctga tttagcctta gctgttacat taaccttttg gggcttagct gggtaatgga
    14221 cctgtatttt cttttgcttt tgcaattctg aatgcactgt ctaatgtacg ttgctgatca
    14281 taccatgctg ttaatacaaa caaatattcg ccttttttaa catctttatc agtgtcataa
    14341 gttgttccat catagttaat tccaaaataa ttgaaatctc ccacaatagg tttaactgct
    14401 gcatctgtaa atactactgg tttgccaaat actttttctg ctggtgtgtc aaagaaattt
    14461 gttgttccat ttgaaagaac actaataatt ttgacataat ctgcatatcg catataaatt
    14521 gttgcgttat cacgataatc ttcatgtaaa tctgctaaag cgttaataat agcatcatac
    14581 atgtctgctc cctcaacttc tttaacagat ccattataaa atgacatgtg ttctaatcca
    14641 gatttaggac ttactgctaa ggcatcttta cgctctttag ctgctaatcc tgattgtagt
    14701 gcgttttcaa cccagtttac taaatctaca tctgatccat gaattacagt atctgaaatt
    14761 gcagcaaata ctttgaattt attagtagtg aacttgactg tatcaccttt tgcttttaat
    14821 tcttttgctg tttctacgtc tgtaatgaaa tcatcatcgt ctaaagtgta tgaaactctt
    14881 ggaatctcta aacctttaat gttagttaga cgagcttttt cacgtaattg gtttttagca
    14941 aatggttctg aaacaatttc tttagaaagt gtttttggta agagcttatc tccacctgaa
    15001 tcatttcctg ttggtaaagc gtgtaataaa cgttgtgcct ccattgaagg tttttcaaat
    15061 tcatttggta aaatcgcgtg acgataaaac tctgccttag ctttaaccat cttctcatta
    15121 tcacttaaag attgataagc ttctccttta tctttaactt tcgctttttc tttctcttca
    15181 atgtcttgca cttgtctttc aacaatgtta aatctttgtt gtaaacctgc tttttctgtt
    15241 tctagttgtt tgatgtcttc catatcaata tttggatctg ttgctttctg actcaattca
    15301 tcatttttat tttttaattg ttgtccaatc atacctaagg attgttttaa ttcatataat
    15361 gtcggcattt catttcctcc taataattca ttgtcatttt taaaatttcg cattcgcgtt
    15421 taattttttc tcttttttct ttttcttcta gtgacatact ttctttaggt gtttcaacca
    15481 attcagatgt atctacatca tcaattttag tgattttgtc tacatctttc tttaaatctt
    15541 ctgggacgtt ctcgaaacgc ttatattgct ctttagagat actagcagct atttcattag
    15601 ctcctaaaat ttcatctatc aagccgaaag acaaggcttc ttctgcagta agccaagttt
    15661 ctgcatctaa catctgtttt aagtgttctt gatctaaatc ttttgcttta tctaaataag
    15721 ctgaattact aacagcatct gttttttcaa gtaaatccgc tgtctttctt aattcttctg
    15781 cattacctac agtcataacc catgaattat gaatcattaa aaaactattt ttgtgcataa
    15841 aaatagtgtc accactcata gcgataacac tagcaattga tgccgctaag gcatcgacat
    15901 agatattaat ttttgcagga tgcattttta gcatattgta tattgcatgc ccttcaaata
    15961 cactgcctcc agatgaattt atatgaacat ctatttcact gatgtctcct agttcatcta
    16021 gtttattttt gaaatctgta gcagttacat cactttcaaa ccatttatca cttacaatat
    16081 caccataaat aaatatttca cctttacttt ttgattttct tttcatttga aaatacttag
    16141 ctttcattga catttttatc accacctttc aaagattttc ttaattcaag tggcgtgtca
    16201 attgggtata aatcaccgct tattagcggc ttatctccac cttcaactgg tggtaaatct
    16261 tcccactctc taatgtcatt tatagtgtag taaccactac gaactgcttt aaagtacact
    16321 tctgcttgtg ttgcactatc agcccttaaa taagatttaa cgttaaattt aaaataccta
    16381 tttttttctc tgtctgtttt agtaagtagt ttccgattaa attcttcttc atactgtttg
    16441 acgattggca ataaggtatg ctgcaagtaa aatctgttta actcttcatt tttcgcgaaa
    16501 tttgtatttg atcttgcatt taagaatact gagggcaatt gaaaaacgtt agctactctt
    16561 tctcttgtta aattctcgct tgccactata tcttcagaga catatttttt aggtaacggt
    16621 tcgatttcaa caccaggctc ttggaataat attccaccgt tttcttcata gtactgtttg
    16681 aaatcttcta acacttgctg ccttttttct ttacctacat tggaaccata tttaagcatg
    16741 aaagaatcag gtttttgcat ttctgtaaga ttaaaggttc ttactgcatt atcaaaatca
    16801 gttgtattct tcaacacatc aatcggacta atgccttgca ccatattaga tgccacgatg
    16861 tgtttaaaat gcaacatgtc catattatga acaatcaatt tatttccagt tgcagcatga
    16921 atggaataat aaagttcacg tgattggttt tcaattaaca tttcaacaac atctggattt
    16981 aataagaaaa gctttgatgg ttgatgatag atgtctcgtt caattagcac atatgcatta
    17041 cctttttcat ttctgattgt ttcaatttga ttaataaaat caaaactgct cagagaatta
    17101 ttcggtgaca ctgtaagtaa atcagatact tctgtattaa ctactttata atcttcatac
    17161 attttcaagg gcaaactagc catcgaatta gataactttg taatagctga aaatatcgtt
    17221 tcattagttt caagcgtatt attgattaca ccccaaaaag atttattttt ccatgggcta
    17281 aagtcataaa gcttagaagc tgactgatca atccaattgt ctatcaattt tttctttatg
    17341 cgtgtgacaa tattctcttt tgcgataaca ttcacctcct taacgcatta tatctttaat
    17401 actaataaat tctatgtttc cttcaccctt gtcagaaaca actttattca taatatctgt
    17461 atatgtgttt aaaaatgctg caaagccatc tattttacga tatctgcttt gcttagacgg
    17521 caaccagttt ccgtttctgt ctagtttcaa ctgaacatta ttgatatacc atttcattaa
    17581 aggattatta ttaaatatta ttttcccatc taaaaacatt tcttttaaat ccttcaatgc
    17641 agggctcaag gtcaaagctc cttgtcttgt ttcttccgtt tcaaacccgt aattttttaa
    17701 ctcttgattt agtttgaatg cgttcgctct atcataagta attttttcta ctacataatg
    17761 ctcattcatc ttaattatcc aatttaaaac atcttggtag tcaatataag gcttatcttg
    17821 cactgttaat aagccatctt cttcccattc tctatagggt attttttcgt tagaatattc
    17881 aactttgtgc ttaggaatcc atgaatgcga taaaactgca actttaccat tatctaacgc
    17941 aaaagtagca cacgcggctg taaagtcctc tgtttctgat aaatcataac caatcgtgca
    18001 tggtctgcct tccagctctt ctaaagaaac aatttcatta tttttttgga gtgttgggta
    18061 atcaataaaa ctcatctcgt cattattagc aaagatatta aaccttttgg ttataaaatc
    18121 tccacgttca gctggtgttc tcttagcttt ttcccactct tctttcatct catctaaatt
    18181 tatagagaca cctaagttgg gatttgcttt tatccagttc gacgaatcat taatatcatc
    18241 gtcatcatcc aaagatgcta aataataaaa agttctttcg tcttctatga tttgatctaa
    18301 ggtgtctctt cccgcttcta ccatatcaac aagtggacca tctaattgat accctgctgt
    18361 cgtaatgtag atgagaagag gttgtaacct tgcagctctt gagtttttta taactgaaat
    18421 caatttatag tctttaaatt catgaatttc atcaaaaatc cccatgtgtg tattcaatcc
    18481 atctaactta tcgctatctg atgcttgggg cataattttt gatatcgttg cgtcataatg
    18541 gatttcatct cttaatgttc tgaaattttt atcaagcttt gggctagctt ttatcatcgc
    18601 cttagattca tcgaataata ttctagcttg tttcattacg tttgctaaaa gatggatttc
    18661 agcgccgttt tctccatctt gagaaacagc atagttagca acaccagata tagtagttgt
    18721 tttaccattt tttcgcccca taaatatcaa agcttcttta aacctgcgca gttttgtttc
    18781 tttatgaacc caaccaaaca aactgccaat aataaaatgt tgccatggtt gtaatacaag
    18841 ttgacgttta gatcctttgg aaggtttaca aaacttttct atgaatcgaa taggacggtg
    18901 cgctaattct tcatcaaata cccatttacc tccattttct agatatctaa ggtgtctctc
    18961 acattctttt ttaacatatt tgcttgtttt tattttccct tgagtgactt gctctgcata
    19021 ccatgttgtt aatagttttg gtgaaggttc atttaaaact ttaatagtca ccgaatccac
    19081 cttcttcttg aactatcttt tttctttgtg ctgcagttaa acccatagac ttgagtaagt
    19141 tatttagtgt ttgaactgtt tttgtcagtt ctatgcttaa tggattctta acaatattgc
    19201 tcgcaccagc cttgtttgta tgctctatca tcaaatcact atttttaagt tcatctctta
    19261 accgacaata aaattcatac gtttctatat acaaattaat taatatgtca tcagattttt
    19321 tataatcctc tatatattct ttcagctgtt tttttgttaa tttcatataa agaccccctt
    19381 tcataaaagt ttatccgcgt tgcaagcgaa gggcccccgc cggtacccgg cgaaaaaaca
    19441 ttttaagcca atgggcaggg ggctataaaa ttttatttaa atgttttttt atttaaattt
    19501 ttagaactct aattttctta agattacttt tgtcattacc atttgcatga attttgttat
    19561 gacagccata acaaactgac attagattat ctaattctaa agctttgtta aaatcttcat
    19621 caacataaat aatgtgatgc actatgtttg catctgttac aatatcttcg cgtaaacaca
    19681 tttgacaaag atgattatct ctatctaatg ctatctccct taacttcttc catgcttttg
    19741 aatgatagaa ccaatcgtat tgatatgact tacgaccatg cttataaatg ttattgtgct
    19801 tggtcatctc ttacacctct ttgattgcat aacaaaagac acaccgcata gcgatgtgcc
    19861 tcgtgtactt gtgtcgtata acttttagat aactttatac atctttccga tactatcata
    19921 ttactacaga tttgtaggcc ttttgcacaa tctttgcaca atgttatttg atacctgcat
    19981 gatacgctac cgctttaaca aagttctttc gtatagtagt aacagtatta cgatgcatat
    20041 ggcattcatg tcctatctgt tccatcttta acttcttttc tttattccaa tacttgagcc
    20101 ttattacttt cttatgatct tcaggcaact ttaagtattc actctcaact gcttcgacca
    20161 tttcttcaag gtttcgtaac atcttattag ttaataatct agttgccatt agttcagttg
    20221 ttctaactgg ttcgcctttt tgtaatggtc catatacaat attggaatct tgttccttcg
    20281 ttggattaag tatttccaac ctcaatcttt ttatttcttt cttgttctca tttaaattat
    20341 atatttctga ttcaatatat ttaaatgtgc ctggcttgat atcatatatt gtgttcccca
    20401 tgttagacct ccattactta tgcttagcta ttcttgcttt aatagctttc attaattctt
    20461 cttgcgttag ttctttattt tgtaaagctt tatatactct ttgatctatt gtgttatcgg
    20521 tcatgatatg atgaataata gtcgtatgat tttgtccttg tctgtataat ctagcatttg
    20581 cttgttggta taattccaag gaccatgtaa gtccaaacca aacaataatg tgcccacctt
    20641 gttgtaagtt taatccatgt cctgcacttg ctggatgtgc tataagcaac ttaatgtctc
    20701 cactgttcca acgttcttta tagtttgaat cgtctaatgt ggttgcttcc ttaaaccttt
    20761 gaagtattct ttctttatcg tgtttgaagt tataaaacaa tagtattggt tggccttgag
    20821 actcctctat aatttcctct aacttatcta acttcttatc atgtataagt cttacatctt
    20881 cctcatctgt ataaactgcg ccgttagata gttgaagtag tttctgactt aatgatgccc
    20941 cattttgagc tacaactgtt ccttcttctt ccgattctaa aatatagttt ttttctaatt
    21001 cttcatatac ttttctttct ttttctgata agactactgt ttgtttagta tcaactctgt
    21061 caggcatatc cagataatct ttcgctttca tgcttaaaca tatatcttct atttgtttat
    21121 atatcttttc ttcagatccg tctcttagct cccacttaaa aatatgttcg ctaacttgat
    21181 gagttggttt aaagtacctt tctcgataac gactgaatga agactcaagt ctttcacctc
    21241 tgtctatcaa ataaacttga gcccataaat cctgtaaact atttggacta ggtgttcctg
    21301 ttaatcctat aaatctatta atgagtggta atttcttttt aatagattta aacctttgac
    21361 tcttaggact tttaaatgta gacagttcat caatcacaac catgtcaaat ggccattctt
    21421 ttttatattg atcacataac catttagtat tttctttatt ggttacatag atatcagcct
    21481 ctgtgtttaa tgcatcattt ctttctttag gtgttcctaa aactaaagac actttcagat
    21541 gatttaaatg gttccactta tcaacttcat caacccatgt atctttagca acttgtttag
    21601 gtgctataac taacattttt ttagtgtcta acaactgcaa ttcactaaat gctgtaagtg
    21661 ttgatactgt tttccctaga cccatatcta aaaacagacc gtatttctca ttatctatca
    21721 ctttatctat tgcatacttt tgatagctat gtggtttgaa gtcaatcgcc aaatgttcca
    21781 cctaccattc tgataaaagt atttacttgt tctttattcc ataacacata tactttatga
    21841 tctctatttt caaattgtct atgcacatat ttttgtaaag gatgcaactt tcctttttct
    21901 tgcttcattt ctacaaaata tgtttttcct tctggcataa taataattct atctggcaca
    21961 cctcttgttc caggtgcaac ccattttaaa cataaaccgt ttagctttga tatctctttc
    22021 actaaatatt tttctaatgt cgattctttc atatattcac cttgtataca aaatttatat
    22081 ttgtgttccg atgttgcatc aattcttgcc aaacttttaa aaatagctgt tagagggtta
    22141 cccctatacc tctttactcc ctaacactac tttttaaact ttatagtgaa tttgatgcaa
    22201 cattggaaac aaacagggtt gaaccttaca gcgagaaggg aaagaggtgt tgtatcattt
    22261 gttgcatcaa tgttgcatca ctaaaaatga tgcaacacct acgattactt tttacactca
    22321 cgtgttgcat cactcaaaaa atgatgcaac atctgataca acactctaaa atgtatattt
    22381 attcaatatt tattatatta aatcttctaa actttcatct cttacataag ctatctgtac
    22441 accataatct tttccaaatc gaattttccc acttttatta ccatcatata cagaccaatt
    22501 gtctaattgt cttaagatgt ttgaaatctt tctaatttcc atagatcctc tactatctcc
    22561 cttatcttta ccaaaacatt caacaaacac ttcaagcgca cagaccttat ttctttcaac
    22621 gtaatctaca tttcctgttg gtaacatatc aacatcacct tgataaaatc gtcttcgttc
    22681 aaagatagtt aagtcatccc aattgctagg aattggtgtg ttaagatatt catcaataat
    22741 gcctgtatat ggagattcct cagtatgttt gctttgtatt gaacgcattt cttcttctag
    22801 ttcagggttg aggaataact cttctccttg ttcataataa tatttagctt ctgcccaaat
    22861 ttggtcgatc tcttctttgg ttagtttaga ccagttcact tcaactctct ctggatttac
    22921 agtcattggc caaaaacgtc ttccaccagt ttcatctctt aagaaatcaa ctttattagt
    22981 tgtaccaatg aaaatacatt gccttggaaa atcttcaata taatgtccat aagcaacacg
    23041 aaaccggtca acttgtttag atatgaaatg cttaatagct tcaacttcag cttttcttgt
    23101 agctgcaagt tctgccattt ccattagcca aacgccttgt aaggcctcat aggcttcttt
    23161 acctgtaaca gaaactaaac tgtcagaaaa ccatgcacca cctaattttt ttagcaaagc
    23221 agatttacct acaccttgag gaccataaag tgtaagcata tagtcaaatt tacatccagg
    23281 ctccattact cgagcaattc cagcagtcaa tgcttttttg gtagttgttc tattcacttc
    23341 agtatcttca acacctaagt atttgataaa taacttttca agacgtttat gtccatccca
    23401 cgatatttta tttagataat cccttactgg atgataagca ttttgcattg ctacgcttat
    23461 aatggcatct tttgttttac ctgaatggtg tatgtcataa atcttttcga tataacttct
    23521 taaactgcta tcatcaccgt cttgccattg acgtgtctta aaattagtat tccatggcac
    23581 tttccctaag cattcaattt gttttgtaaa ttcattaaat gctatttttc cttttaaatt
    23641 tggatcatta cgcaatataa tttctatatt tgggatacta gctttgaaag tacctttcga
    23701 agtaatttct aacgtctcag accatgcatc atcgctattt actatttcat cgaaatcctg
    23761 cattgcatca gacattttgt cgttaattaa ttgcttttta acaacttcat cattttgcgc
    23821 tctttgctgc attgctttat aactaggtag tcgattaacc ggagtatctg ttttagcgtc
    23881 ttcatcttga gcaccatata agtgtatgcg tactaaatca aaactgttca caagcatacc
    23941 gctaacggga tccgtattat gatgagaata ggcaaacttg ttattttcgt ataacaccaa
    24001 tccacctgca gttgaacctt catgataggt ataacggtta gtagaatgtt tttcgtataa
    24061 gtcaggaata aaagttgata tagcttcttc tatcgtatag gctctacaaa atgcgccaac
    24121 aattcccggc ttttcttctg ggtcaccttg cttatctgct aatcttttag tcttactctc
    24181 ttcctttgaa gacgttggcc attctaatgt gtcagtccaa tcaacatatt catttaatat
    24241 tttatctgga tctaacaaag gtaaatcttc ataggtaaag aaaaattctg catcattgct
    24301 agttgaaggc caatacatta acctatgtgg ttgataagtt gtatcatcga agtaatccat
    24361 gccaacgata tctgcgactt tacgcccaat agcttcatac tcatctgcat ttacatttcg
    24421 ttttaaagga atcactaaac gcagtcttgg acttatctct ctatgcttat gtgttgaata
    24481 taaacaatat gcaaaatcat aaaacataga taatatgtca gtcatatctt gagcagcata
    24541 atcgatatca agtgttagca ttgaacgatt catgacttga ccagcacgtc gtttgccttc
    24601 ttttaaatat ccaccgacaa atccgccaac atcttttata tctgcttgtt cagacttaga
    24661 cattttattg tactcagtta aatcttcttt agttctaact gtttgtgcta gcttctgcat
    24721 aaagtcagac caagccatat tgtgattagt ccaatatgtg gataaacgac tagcagcata
    24781 agaatatgag acatcacgat catatttaat tgtttctatt tgagtgactt tgtctaacat
    24841 attcggctcc tttcttgtat aattaattta ataaaattaa agaggtgaca aattttgaaa
    24901 actttacgcg aaatcattta tgataaatac gaaaatgaaa acttgagaac tttaatttta
    24961 ttgcactcag gaaaagcctt tcccgtaact aatataatct cagaaaattc aaccttctta
    25021 attatcaaga cttcaaagac aatttcacat aaattagttt taatttacaa aaacgaaata
    25081 aaaaatatcc gcttctttta atcctggaga gggctttagc cccctcttta gcttttgaat
    25141 ctttttctaa ttcgttcaac ttcattttca taatcttcta aaccttcaac accattattt
    25201 tttactaact gcttgaaaag ataagaattc atatactcca atgcttctat ggttttcatc
    25261 ttatgagaaa tgctacttaa caagatcaat aaaaatatag ctaaaacgat tgaaatgaca
    25321 atccacatat ttacaacacc tccagtgcta ttgctaaaca cattaatata attaattcaa
    25381 aaatgataat agctattacc atgaaacttc agctctgatt ttttcgtaat cactcggcgc
    25441 ctctacatca tcattagccg tcatcataat atacacttgc tcagttacat acttacctaa
    25501 ctcatacatt gctagtaaga ataatagtct tagtatttgt ttaatcattg tttatctacc
    25561 ttctttactt cgtataagac cggatataaa tttaaaaagt gtattctata accgattgtt
    25621 ttaacttcta ccttatcgcc tacttttaac ctagcttgta tatctgcgct atcaaatttc
    25681 tttttgaata ataagtcgga attttcaatg acttgtttgt tgtctaatac aatatagaac
    25741 ttgtcttctt tatcttgtct cttgttatat ttatctgtaa tagttccttg atgtaattct
    25801 ttgtgttggt aactagccac tgtatagata ggcaatgtga taacaagtag caatgcgaat
    25861 ataccgaata atgacagtat tccaacaata aagatgtcga acccatccat atttttaagt
    25921 tttttaatca tcattgtcat ctccagtatc aattaaacta ggcatcattc ttaacatagc
    25981 ccttaattca tattcattca tattagccat cgtaggactg taaaattcac tgtctttatc
    26041 tttaatttct ttaataaaat catcttcaat cttagccttt tcttcaggtg ctttattttt
    26101 atattttttg attatttcag tgtacttttt cgggaatttc attttaggta tgttaatcat
    26161 cgtttgcctc cttaataaat gtaaatgatt caatctcatc tcttttaacc catacttcat
    26221 tgttgaacac atctttgacc ggaagaaaat cctcaatcac tagattcata acaagattaa
    26281 tataatcgtc agaagctaga tctgttgttg tgtaataaac tctatctgaa atagttttaa
    26341 ttttaacctc cgtcatttcc cacactccct tatattttca aataactgac ccactttaat
    26401 aactgcatct cttttaactt gtgcctcgta tttgcgctca gcttcttctt tactctctgc
    26461 ctcaacaact gtaaaccttt gattgctttt agctttagtt atgtgtgtat gcttgcgtcc
    26521 tgttgaatct ttgaatgttg tgactaagta ttgcgtcact tccccaaaac ctccttgact
    26581 cgatctaaga tgtctttaca cgtatccttt tcctgcgtct gctgttccat cttgtctttc
    26641 atgattcctt ttcattttct ttttgtatgc gtcaatgagc tggtcgatag aatataagtt
    26701 gaaagctatg tctatcgcta ttacaattgc caattggtca ggataaaatt ctttgaatat
    26761 tatctgtggt gtgctaacaa ctgcgtcttg agcaaattct ttatctttaa aattaaacat
    26821 gttgtgaaat tcactatttt taaaacttga ttcaatcgct tcttttatct cttctgatga
    26881 cactcctact tgattcgcaa tactcaatcc aaacgccaac atgtccgcta attcatctaa
    26941 ctgaacatct aacggcttac ctggtttctt cttccagttc ttaaacgttt ccaatgtatt
    27001 aaaccattca aagaattcaa ctacatatgc tattttgcta tctcgtaagt tcagcgttgg
    27061 tattctatcg tcgaactcct tttgtatttg taataactct tgtaactgat caattgttaa
    27121 tgtattagtc attttcctga tcctcctcat atttatagac cacttgcccc gtcataatcc
    27181 ctactgcttc atcaagacca atatcttctt tgagtgcatc ttgcatagca ttaggtaaac
    27241 cttcaagtat ttcatcaaac gcttgcgctt tcttatacac gtcttcaatc tcttttagta
    27301 atccctctgt gtcattgccg ttatacgcac tagcactgat aattgattgt tcaatttgtt
    27361 cgcggttatt catcatttcc atctcctcta aaataaagtt agttgcttct gttcctcgta
    27421 ttccaaacca tgttgcttta tatatatttc gagctcttca gcagtatcaa atgtcttttt
    27481 aacgctttgc caacctggca cgatatgccc atgaaagtaa tacgtgccat ttactacatg
    27541 agtatgtgcc actcgttcgt tatcctgata cagatatctt ttagagccga aaaattggtt
    27601 taagcgttct ttacgtgcgc tatctgtcat ggtcgtcact ccttttaaca attaggcagt
    27661 ccaaacgaca tgcattcgta atatagttct tcattcctta tgcttgtctt atagttctca
    27721 atcacattgc taacttcttt atgactcatt gctttaactt gttcgtctgt atatttttcg
    27781 cagtcttcta attccagttg ctcctgtaat gacattacgt aatcaacttg tttttgcgtt
    27841 gccatcgtta cccctcccac aagtcaaaca ctctatcgac gtaaaacttc gcctttgcta
    27901 aatcctcatg accattcttt aacggtgctc tagacaagta tttgattgca ttacctattg
    27961 cgaatgctaa ttgtggtgga tactgtgccg taacttgttc gatgaaatct ataatttcaa
    28021 tgtcgccgta tgtgtaatgc gcaggttgtt taacattgtc ttgtgtttca ttcatatcta
    28081 cttttctgtt actgattatg ctcattatgc ttcactccat ttcttgaaca tttggttata
    28141 agtgacatcg aaccagtacg gatcacgtga atgtttttga ggcgttccat cataaagcca
    28201 tggtctcaat cttctctttc tttcttcttc atattccgct ctcacatttc gttggtatag
    28261 gttcaaaatc gctttttttc tgattttttc tctctctttt tcttcatctt ttatttgact
    28321 cttcatatat tcaacttcat ctttagattt tgagtctttt cttccacaca ataattcatc
    28381 gccgcgcatt ttatgtttgt atctgtatct aagaagttct ggagatatat gatatttttc
    28441 tgaaacttct ctcaatgtca ttagttttcc tttgatacgc actcttataa cttttcttct
    28501 agccatcatt ccacctctaa atctaaaacc ttgatattta taacgttata ttttaatagt
    28561 tcacctggat tattaaataa atagtccgcc aaattctctt tttctttatc aatctgattg
    28621 taattaacac tttcgacttc tgtaggaatt ctaatgtcaa cagaagcatt gatataagct
    28681 tgatgttgca ttcaatcaca ctcctaatcc ttcatataaa acggagaagt aaatccgtca
    28741 ctattcaaat tcaatccttt tgcccaatcg acaggcttat tcatgatagt ttcgatttcc
    28801 ttaagtccat ttgaacctct aggtatttct acaattactt catcatggac atgtccaact
    28861 attttaaaac ctgatgcttc aagcctagct atagaaatcg caagtaaatc ccttgcagtt
    28921 gcttgaacaa tattctcgac taacttccca ccatacgttt ttaactttga ccatttacgg
    28981 ttaagatcta agcccataaa ttcaacaact tgactacccc aactattttc accaactgaa
    29041 gcttttggat aagctaaagc tcttccacta ggcagttcaa tcattagaaa accttttttc
    29101 atataaaatc taactccatg cgtatgatgc gtctttcggg atttcacagt attaattgca
    29161 gcctcttggc aagccttcca aaaattaact atgttaggat ttgcgttacg ccaactatca
    29221 actaaacctt gtaattcatt ttcttcaatg cccatttcca atgcgcccat cgcttttaaa
    29281 gctccagcgc caccttgata acctaaagct aattcggaca cttttccctt ttgtctgaga
    29341 gggtcgcctt tagttatgct ttctaccggg acattaaaca tttgagaagc cgatgcttca
    29401 tatatctttc cgtgtgtgtt gaacacatct aaacgccatt gttcttttgc ataccatgct
    29461 atgactcttg cctctattgc agaaaaatca cttactgcta gttcattacc ttcttcagca
    29521 gtaaatgtcg tcctaactaa ttgacttaat aagtcttgag gatgaacatt gagtaataaa
    29581 tctaaatcgt caaaacgttg ttctttaata agatctcttg ctatttctaa ttcagtatct
    29641 gaaatataat gctttgttaa attctgaagt tgtacacctc tacctgccca tcttccagta
    29701 ccggcaccgt aaaattgaaa cagacctctt acccgttcat cactgcacat catgtcatgc
    29761 attttgttgt attttttcac actggtttta gacatttgca atctaatttc tagcattttt
    29821 ttagcttttc ctgttgcttc ttttaagtac tcctgaaccg ttttcttttg taaattaggt
    29881 atatctaatc cttggtcatc ctttaaccaa gccaataatt gtgtaggact attaggattt
    29941 tctaaacctg ttatatgttt agcttgatta agcaattctt ctttactctg cttatcgagc
    30001 acattagctc ctaacatcaa tgatttagaa agcttaatac ctctgtcgtt tatatgttgg
    30061 tcaaaaaccc aatatgcttg ttcaattgca gttactggaa agtctttaat tttatgagca
    30121 atcgtcattt ctacttctac atctcgaata cagtaatcta taaattgttg ccatttttca
    30181 agatcatgtt caggcaagtt tcttgttctt cctccattaa cttttgttgg tttacaaggt
    30241 atagagaaat aacgaattaa atttttacct gctttatctt tttggttttg tagtcttaaa
    30301 acttctccaa ctttatcaag cgaagcaggt aagccaatac gcattgaatt aaccattgtg
    30361 caaatccatt cttcaggtgg catctgttta ttaaaatgtt tagcaagaca agttctttcg
    30421 aaattagcat tgaatgcata cttttttaca gcaggatcaa aaagagcaat tttaaacgtc
    30481 tcaaaatcag cgtggaaagg ctcattatct actttagtca tgtcaatcgc actaatcgct
    30541 ccaccatcta ttgaataagc tataattaaa atttcgaaat cttcagcttc tgtgtattta
    30601 taggcaccac atttcgaaat atcattactg ctatatgttt caatatctat attcataaat
    30661 ctcaaattct tgacacctca atttctttaa aattaaagtg gggctaaaac cccacctatt
    30721 gacttataag aaatcctcat catcagtgtc taattcatcg aaatcatctt ctgctgcact
    30781 tgcaccgcca agaggttcgc ctttttctac aagttgaatg ttgttcaatc caactgcgat
    30841 acccttatta ccatttgtgt tgaaaggaaa tagattaatt gaagctctaa tataatcacc
    30901 acttacaaca gttccagaat ccgttaatct aattttgttt tggtcaataa taccaggtgc
    30961 ttgtttgctt gatgcgttaa taaaataagc gtcttgataa ttgacatcat cttctctttc
    31021 agtatctcca tcacgtaatg gaagtttcag atttgcagga actttgcctc caaacttact
    31081 aacttttcct tcttctttag cagcttctat agcttgttca atggctttta tcgtacttgt
    31141 atctgattta ggaatgatta aactgattga atactttgat tcttgccctt cttgcatact
    31201 gtgaggttca aaaatatgtg catatgatgc tcttactttt cctgtaatca ctttagtttt
    31261 atttaatact tttgctttca tgtttatata ccgtcctttt aaatttttat agtttgtcaa
    31321 aatcatcttc agcagattgc tttatagctg gtcgtttatc cgactcggta gcaagtgtta
    31381 atttaccttg cggcttttct ataaaaccct ctgtaatttt agaaaatgct tttttaccaa
    31441 ttaatttttc taaattcgta atgctaagta acttggtttc tgtaatatct tcaggtttat
    31501 aacccgcttc aactaacttt tcaagcattg cttttgtatc agttatcatt cttcgtgaac
    31561 gaccttctac aagcttccaa ccaggatagt ttttatcatt ttctttcgct tgatttagtg
    31621 catattgttc tacttcatca gcccattttt tgatatcagg cagtttatat aaaagttctg
    31681 caatctcttc atcacttaac aaatgtggtg gcttttgagg cacattttgc atgtattctg
    31741 cacgtgttct acatgaatga tttatcttac agaatctaca atgactacct gctttaaact
    31801 ctcctccacc gttataagca agtctggcta atggtttaac aaaatcggct ccccattgaa
    31861 gtaatcttga tattggtaac tcttcagtag aaaagttatc tattcgaggt tgtatgatag
    31921 tcatgcgaat tgtatgaatg tcatacatta aactaagcag ttcatatgcg cccaagccat
    31981 ataatctaag ttgaggatta tctatagctg aaacttcaat gcctttacca tatttaaggt
    32041 caataatttc aagtacacca cctgaaaata taatgacatc accagttcca aaagattcag
    32101 ggacgtattt acctaaatct aattttgttt caaataaagc tattacatca ttatccctac
    32161 tcaaagcttc gttatatttt tcttctacat tagctacata ctcttccaca tattcacgca
    32221 attcttcgct gtaatattga tttctcttat aattttgaaa agccttatta aactcaaact
    32281 gtgttaggcc ttcatattta aggctgaaat ataactcact taactcatgg gcgaatgtac
    32341 cttcttcggc aaaaactgta cttttatctg caataccttc acttgcctta atactcggtg
    32401 ggcagtttag ccattgtttt gctccacttg cactaagctt tgcatgagct ctatttgagt
    32461 gatctagctt catgcattta ttctcgcatt cataaaatca acaatttttt cataatgctc
    32521 ttctttgata gtagatagct tatccgcacc aagttcgtta agtttatttc taaattcttt
    32581 cttatcagaa gtatctgctt ttttaaggaa ctcttttcct actgataaaa cataatcttt
    32641 agttaaatca gcagaagttt ccttaacttc ttcaattgat tccagttgag ctgtttcttc
    32701 ttttggcatt ggtgcttctt taactttctc ttgtacaatt gatgaatcta ctgttgatag
    32761 ttcagtgttt aacacacgta aattgttatt taatagtttt aattcttcaa aaatatcttc
    32821 taatattgcc attgattaaa tcctcctacc atttcatgac taagttaatt agtctgtcct
    32881 gttcatctgt gttctcttca atccattcgt ttataacgtc acgcattgca tccgtcgcaa
    32941 aatatagttc gcttaaatct acaacatgaa atgatttaag tggaatatta ttcatatcct
    33001 tgatttgtat actgataccg tcatgttttt tcatcgcaga cactttaaat tcaaacccgt
    33061 taaagctgat aattttattt tttatctcac caaatttgta atacattgtt ttagccctcc
    33121 ttgttatcat caataccgtg aaatttttgt gatttacaca tttggagaac attgacaatg
    33181 tctttataac tcttagtgct atccaataag gaagcaagat cgaaagtatg accaatcaca
    33241 gaacttgaac ctgctaaata atctccgtcg ataactccta ttgatgagaa aagcaaaata
    33301 tcaaatttac tttctccctt aatttctttc gctaattcat acaattctcc gcttttttca
    33361 gataataagt cttttacttc ttcctgagtc atgtctttat attttttagt cattgttgac
    33421 ttcctcctcg ttttcttcgt cctcctcgtt atcttcttcg ttttgtaatt cataaatttt
    33481 gttttttagt tttatatttt ctttttccaa tttttcgttt tttctttctt ccgcaaaata
    33541 cttacctctg taagtatctt cttctttatc tttaacagcc tttatttcaa taagttttct
    33601 gtactcgttc aatgtgattg ttactgtcaa ttcttgattt gctacaaagt tatcttcttc
    33661 atttctgtat cctgagaaat ctttagtgta ataatgttgt tcagttttaa tattttcagc
    33721 catagttgac tacctccgta tattttgatt taattaagtt gtatattttg ataaatgttt
    33781 gtcactgtta cttgttgacg caagtagcag tttttttatt cttcataaaa gtattctttg
    33841 tagtatatga atgttgcgat acttgcgaat cccgcaattg accatgctgt agtgaagtac
    33901 agcaatggca taagcacaat cgctaagact gtgaagcaca gtactgctat taattagctt
    33961 ttatatgtgt cgctcattta atatcctcct aataccattt tttatgcttt ctgatcaaat
    34021 actcttccaa tttagaaata ttaatcagag tgcctgttgg tgaataatca atgtataaat
    34081 tttctacacc taaattatct ttgcggtaat atttcaacca gttgtatact gtacttctac
    34141 atactccaaa caattgatgg atttgtgtag gtgttgcgta taactttttc acaaattttt
    34201 cttcgcctct atatgtgttt tctggtgttg gtggtactat gatttttggc atctctatca
    34261 ctcctttcga taaatgttaa agtttgttat tattcgctct gtattgaagt tctctatcta
    34321 atgcatagaa aactttgttt atttctaagt agctgtaatc acctttttta atacttccta
    34381 atatttcctt tcttagtcga cgttcatttt ctgttaaaga ttctactgac gcatgatctc
    34441 ttctgaaaat ccttggtatg attatgtcta acccttctga ttttttgttc atttgttgtt
    34501 ccacctttcg tgtataatgt tgttatcaac ctaaggaggt gataagtata cataagagat
    34561 tgctcactca atatttagat aaagaaatcg ttacttcttt agatttacat ttaattaatg
    34621 gtgaagttat taaagtacaa gaacatataa aagatgctga aagcaaaact ctacacatca
    34681 ttcatccaaa agatagagtt gtcagtttag atcatgtttt gtatttcgac attaacgtta
    34741 aaggtgaaaa gaataacgat agtccttatc catcttaaaa tccatagtgc ttatagttat
    34801 ttgctatttg ttgacagtta tcactggcaa gtcctacctt gtcagcttgg taactgtatt
    34861 gattatttac ttcatttact aatcgattcc attcgtctct tggtatatct tcaagaagta
    34921 agagaatcat ctttaatttt tcttcgttca tttgttattc ctccttttaa gatgtttgtt
    34981 tttctcctaa aaacttatta acaaagtatt gttgtccttt gcctgttact tttggcgtct
    35041 tactaattga tgtgtgaccg tccgaatgtg tgattgatgt ttctttaatt tcgaataact
    35101 cacgttccat tgaatactgt gtaggcatgt tataatccac acccttgcgt ttaataagga
    35161 atccgttttg acgtaaccac tcaaacaatc tgcgttgccc gatgtttaca ccgttttgtt
    35221 taatgatctt tgctaactct ccaactaaaa ttgatgtctt agtagtagct actgcatctg
    35281 caaatacaat ttttggttta tcacgttcaa tctttgtttc taattgattg attgtgttgt
    35341 tagcaatttt taaagcacgt tgcataatca tttctgggct gttccatgct ttttcaactt
    35401 ggatgaagta ttgtcttgca cgtttaccag gttcactacg ttgaatcatt gcgatttctt
    35461 ttgcagtgtc tagtgtgagt gcgtggtcaa tatagtgtgt catattgcct tgagctgttg
    35521 ctcttttttg agcgatagct gtgtaatctg tattttcttc aaatccgtat ttaagcattc
    35581 ttggaaacca atctttatat gctgtcttaa cttctaatgc ttgatgaagt tctcgaccgc
    35641 tgattgcgat ttctccattt tctttttctt gaatattgaa catttctccg atgttcgaat
    35701 ttgtttgtaa tgcttgcata ttgtttatgc tcctttctgc tatactccta ttaaggaggt
    35761 gaatgactta tgactaatga agctaaattt gtccttttac aactttattc aatttacctt
    35821 gatagaattg acgaaggtat gtctaaacgt tctgcatctt atttcggtag tgatgaatcc
    35881 tcatttaacg ctttcttttt agattttaat tttgaagact atatcgatgc agttcttgaa
    35941 ttaaagcata gagattttgt aattgcttct gctgaagatg gcggttttct tgagatggct
    36001 ctttctcgag aaggtatcgc ctactcagaa tcagaatcca aaaaagatta caaaacactt
    36061 atggatttaa ttagagattt gaaaaaatta ataatctaaa atccaatcat ctgctattaa
    36121 atcgtctgcg ctaggctgcc accttccggc ggcggtttgt ctttttttct tatagtgtct
    36181 agatacgact aggcattgat aacgctgcaa attagttggt aatatcccat acacatcttg
    36241 attctctctc cttatactta ttcctttctc catcgctagc ttcgttgctt cttgaatgtt
    36301 catttgttat tcctcctatt aagatgtttg tttttcttta aatgctaaaa taattgattt
    36361 ctttttatca ttcgtaaata cgaaattttc gtattcatta cctaaaaaaa tatcatcata
    36421 tttaacatta aaagcactca tatacttaga aagtaaacta tctttaatgt ttgtagagtc
    36481 tttttccata ttttgaattg tacgtgatga gaccttaaat aaatctccta actctttttg
    36541 agtcaatccg taatcagtcc tcaactcttt taatgttttc atgttgtcac cgcctttcgt
    36601 aaacctaata taatacgaaa ttttcgtatt gtcaacatta aatacgtttt tttcgtaaaa
    36661 aactttacta tgatatgaaa atttcgtata ataagaaaaa aggaggtaag taatatgaac
    36721 aaagaaagaa atattattat agccaaaaac attagaaaat ttctcaacga ttcaaatatg
    36781 tctcaaaaga aacttgctga actcattaac ataaaaccat ctactttaag cgattattta
    36841 aatttacgtt ccaacccctc tcacggcgtt atacaaagga tagctgatgt tttcgaggtt
    36901 ggtaaaagcg acatagatac tacatacaaa gacgataacg acatcacttc catatacaac
    36961 aaactcacac ctccccgcca agaaaacgta cttaactatg caaatgaaca attggaagaa
    37021 cagaattcta aaggagataa cgttgtagat attaattcat ataaacagga gaaaactcca
    37081 gttaacgtca atggttgcgt ctctgctggt gtaggagaac gtttacacga tgaaacgcta
    37141 tttactgaaa tggttaaagg acctatcccc acacacgatt tagcgttaaa agtaaatggt
    37201 gattctatgg aacctatgtt taaagatggc gaaatcatat ttgtggagaa aactcacaat
    37261 ataaagaatg gacaaattgg tatattcatc attgaagaag aagcgtacgt taagaaagtc
    37321 tttgttgaag atgatagatt gactctagtt tcactaaata aagattacga cgatctacac
    37381 ttttatagaa atgaaagtgt gaggttaatt ggaaaagtta ttttataaaa ggagcacttg
    37441 caaatgaaaa aatatgatat tgcagtctta gactttgaaa ctatgaatga acatatgaac
    37501 agcccttgcg aagttgctgt atctttaatt aaggatttat caatagtaaa agtttattca
    37561 tcttatatta atcctcctaa taatagatat aacttgaaaa acgctaaaat acataaaata
    37621 cctgaagatg tcatattaaa agcacctaaa tatccagata tttaccaaga aattctctat
    37681 cttttaaaag aatcacattt aattattgct cataatgcac tttttgatat ttcagtatta
    37741 aaaaatacta ataattatta tgacttacct gttccaaact tcatgtatgt cgatagtata
    37801 aatatcttta gaagcttcca cgcaatctct agttttaaat tagaaaattt gtgtagctta
    37861 tatgatatcg ataaagaaaa attacattct gctaaatttg acgtgctagc tttatcgaag
    37921 atgttgatat cactcgctaa aaacaatcag cattatagtg tattaaaatt aatacattat
    37981 atgcctaagc aatacattag atttagcaaa tattctaact ctccaactaa acttttcgat
    38041 tcaggatttc aaaaaattca tatgaaaata tctgagatta ataaaataga agtggaaagt
    38101 gtaatcccta ttttaaaaga taaaaatgtt gtttttacag gtaattttga cactgaaaaa
    38161 caagatttaa tgatattaac tagaaagaaa ggagcttata tcagaagtga cgtaactgca
    38221 aaaacagata ttttagtcga aggtgttcaa gatgataaat ataaagatgt gaacggacta
    38281 gtttcaaaac aacgaaaagc tcgagaatat gttggaaatg gtgcaaaaat tcaattttta
    38341 aatgaagaag acttaataaa tttaataaag gaataataac gatgatcaaa aaaattttta
    38401 caaaaaagca tgtattctta gttatagaag atgaaaacca taatcacagt gatgctgttt
    38461 ttggaaaaag tatattactt tcaatttacg tcggtgtgaa taaaaagact aattctaaat
    38521 cagggaaatt tatatacctt gacagatcta aaagaatcgt tagacaatct gatatcacca
    38581 aaatagaatc agctaacgaa aatgatgtag atttttataa tttactgaag aaagaaaagg
    38641 aaattgttta ttccaaaaat atagtagata aatacaattt agcgaactat ataatttact
    38701 acgaagttag tactaaagaa taaaccaatc cattatttca taatactaac cttaaattta
    38761 cagaggtttt aattatgaaa catgaaaaaa gcaatcttaa ctttaagtct tatatttatt
    38821 acctactacc tcacttttaa atatatgtgg attaaagaat tgaagtatta attatgctta
    38881 tttaaaaaag acgtctattt cagcagtgtt tgaaaggaag tttataatga aaataactaa
    38941 ttgcaaaata aaaagagaaa ctgtaatata cgaagtttta actagtggta atcaaccatt
    39001 cacttatgag ttacctaaag atttatcgtc acataatgcg cgtaaatact tggaatttat
    39061 ttcacaaaaa atagatggcg ataagttaac caaagaagat tcattatgat tttactaatc
    39121 aaaaaacgtc tacaagtgta gacgttgaat ggtggtgaga gtgtgagcga gaataaagga
    39181 gaaatgatga cgcataatat agaaaaacgc attaataaat taaaaacttc tggaaatcca
    39241 aaatttaaaa aattagattc agatattcac tatttactca agagatttga aggtgaaaaa
    39301 aaccataaag gtttttatcc aaagtttaaa caaggagaaa tagtttttgt agatttcggt
    39361 ataaacgtta ataaagaatt ctctaattca cactttgcaa tagtgatgaa taaaaatgat
    39421 tctaatacgg aagatatagt aaatgttatt cccttatctt ctaaagaaaa caaaaagtat
    39481 ttaaagatga attttgattt gaaatgggag tattatttaa gattgttttt aaatttaatt
    39541 agcgcgcaaa ataattcagc tatattaaaa gaagttttcg ataaaaaata ccaaaaaaac
    39601 aacacagaat tcatcactaa agattatttt agtgaattta tatctgatag tttagaaatt
    39661 gaaaataaat taaataaaat tgacagaaac attaataaca tagtatcagc aattgataag
    39721 gtaaaaaaat taaaaggtaa tagttacgct tgcataaatt ctttccagcc gattagtaag
    39781 tttcgcataa gaaaagtttt accccaaaaa attaaaaatc cagtaataga ttcttcggat
    39841 attatgttac t
//
LOCUS       DAKTIO010000004        39093 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.004, whole genome shotgun sequence.
ACCESSION   DAKTIO010000004 DAKTIO010000000
VERSION     DAKTIO010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 39093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39093
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.004"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            24..374
                     /gene="sarV"
                     /locus_tag="O0293_000120"
     CDS             24..374
                     /gene="sarV"
                     /locus_tag="O0293_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarV"
                     /protein_id="HCY0279033.1"
                     /translation="MSNKVQRFIEAERELSQLKHWLKTTHKISIEEFVVLFKVYEAEK
                     ISGKELRDTLHFEMLWDTSKIDVIIRKIYKKELISKLRSETDERQVFYFYSTSQKKLL
                     DKITKEIEVLSVTN"
     gene            complement(476..1687)
                     /locus_tag="O0293_000121"
     CDS             complement(476..1687)
                     /locus_tag="O0293_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0279034.1"
                     /translation="MTNQGSLNKSPIFTKSFTINFLVNFIVYLCMYLLLVVIAGYSKQ
                     AFDASDSLAGLVVGLFIVGSLIGRFATGKFVNQIGPKRLLFIGLIALIITQLLYFIDG
                     SLAFLIFVRLINGIATAVVTTATGTIAAYVTPVNRKSEGISLFSLSLVLGTAIGPFLG
                     MLLITKYAIDLLFIICVILGILGLIISLFIKVDFEVTNNKTETNVIDKPRFSIHQFIA
                     KEAIPVAVIMLLIGVTYASILTYLQAFALERHLVTAASYFFICYAIASLITRPIAGRL
                     MDDKNENIIVYPAFIMLFLSFVCLISSYQSWLILLAGACLGLGYGNLSSAMQSIAIKV
                     SPPIKYGIATSTFYVGLDAGVGFGPSFLGLFTHMFSYSEIFGFMAALAIITMLVYFLI
                     HGRHVTRNAMN"
     gene            complement(1680..2120)
                     /locus_tag="O0293_000122"
     CDS             complement(1680..2120)
                     /locus_tag="O0293_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HCY0279035.1"
                     /translation="MLSQEFFNSFITIYRPYLKLAEPILEKHNIYYGQWLILRDIAKH
                     QPTTLIEISHRRAIEKPTARKTLKALIENDLITVENSLEDKRQKFLTLTPKGHELYEI
                     VCLDVQKLQQAVVAKTNISQDQMQETINVMNQIHEILLREAHND"
     gene            2282..3046
                     /locus_tag="O0293_000123"
     CDS             2282..3046
                     /locus_tag="O0293_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0279036.1"
                     /translation="MILKFDHIIHYIDQLDRFSFPGDVIKLHSGGYHHKYGTFNKLGY
                     INENYIELLDVENNEKLKKMAKTIEGGVAFATQIVQEKYEQGFKNICLRTNDIEAVKN
                     KLQSEQVEVVGPIQMERDTHKDGKVKWQLLYIMNQDDDEIKPPFFIQWEESDSMRTKK
                     LQKYFQKQFSIETVIVKSKNRSQTVSNWLKWFDMDIVEENDHYTDLILKNDDIYFRIE
                     DGKVSKYHSVIIKDAQATSPYSIFIRGAIYRFEPLV"
     gene            complement(3038..3289)
                     /locus_tag="O0293_000124"
     CDS             complement(3038..3289)
                     /locus_tag="O0293_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279037.1"
                     /translation="MRTIILSLFIIMNIVAIIMTLSQPLTVNYFSLRVILIFFTFILS
                     IFFILIKSSRLNNILTILSIVLVIIHMGILAHSTYVYLY"
     gene            3678..4943
                     /gene="femX"
                     /locus_tag="O0293_000125"
                     /gene_synonym="fmhB"
     CDS             3678..4943
                     /gene="femX"
                     /locus_tag="O0293_000125"
                     /gene_synonym="fmhB"
                     /EC_number="2.3.2.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase"
                     /protein_id="HCY0279038.1"
                     /translation="MEKMHITNQEHDAFVKSHPNGDLLQLTKWAETKKLTGWYARRIA
                     VGRDGEVQGVAQLLFKKVPKLPYTLCYISRGFVVDYSNKEALNALLDSAKEIAKAEKA
                     YAIKIDPDVEVDKGTDALQNLKALGFKHKGFKEGLSKDYIQPRMTMITPIDKNDDELL
                     NSFERRNRSKVRLALKRGTTVERSDREGLKTFAELMKITGERDGFLTRDISYFENIYD
                     ALHEDGDAELFLVKLDPKENIAKVNQELNELHAEIAKWQQKMKTSEKQAKKAQNMIND
                     AQNKIAKNEDLKRDLEALEKEHPEGIYLSGALLMFAGSKSYYLYGASSNEFRDFLPNH
                     HMQYTMMKYAREHGATTYDFGGTDNDPDKDSEHYGLWAFKKVWGTYLSEKIGEFDYVL
                     NQPLYQLIEQVKPRLTKAKIKISRKLKRK"
     gene            5060..8227
                     /locus_tag="O0293_000126"
     CDS             5060..8227
                     /locus_tag="O0293_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HCY0279039.1"
                     /translation="MIKKLLQFSLGNKFAIFLMVVLVVLGGVYASAKLKLELLPNVQN
                     PVISVTTTMPGATPQSTQDEISSKIDNQVRSLAYVKNVKTQSIQNASIVTVEYENNTD
                     MDKAEEQLKKEIDKIKFKDEVGQPELRRNSMDAFPVLAYSFSNKENDLKKVTKVLNEQ
                     LIPKLQTVDGVQNAQLNGQTNREITLKFKQNELEKYGLTADDVENYLKTATRTTPLGL
                     FQFGDKDKSIVVDGQYQSVDAFKNINIPLTLAGGQGQSQSQSDNKQNSAMSDVNQASP
                     QQNSKASAPNNISGMPTAKLGDLADITVGDVRTSISKTNGKDAVNLQITKAQDANTVQ
                     VAKDVQRKIDTFVDENKDFNVTKTMDTAKPVEKSLYTMVEKASLGTIVAIIVILLFLR
                     NIRTTAISIISIPLSLLMALIALKLSDVSLNILTLGALTVAIGRVIDDSIVVVENIYR
                     RLTDSEEQLKGENLIISATTEVFKPIMSSTLVTIIVFLPLVFVSGSVGEMFRPFALAI
                     AFSLLASLLVSITLVPALAATLFKKGVKRRNKQHQEGLGVVSTTYKKVLHWSLNHKWI
                     VIILSTLILVATIVFGGPRLGTSFISAGDDKFLAITYTPKPGETEQAVLNHAKDVEKY
                     LKQKKHVKTIQYSVGGSSPVDPTGSTNSMAIMVEYDNDTPNFDVEADKVIKHADGFKH
                     PGEWKNQDLGTGAGNKSVEVTVKGPSMDAIKSTVKDIEQKMKQVKGLANVKSDLSQTY
                     DQYEIKVDQNKAAENGISASQLAMHLNENLPEKTVTTVKENGKTVDVKVKQNKQTDWS
                     EDKLNNITLKKPTGGTIKLGDIATLVKTTTPSKLTQEQGDYATTVSAKVTNKDVGGTT
                     RQVMSKINNLDKPNNVKVNIGGASDDINNAMTQLAFAMLAAIIIVYLILVITFKGGLA
                     PFTILFSLPFTVIGVIIALLITGETISVPSLIGMLMLIGIVVTNAIVLIDRVINNEQQ
                     GMEMKEALIEAGGTRIRPILMTAIATIGALVPLLFGQDSSILISKGLAATVIGGLISS
                     TLLTLVVVPVIYEILFTLKKRFTKR"
     gene            complement(8414..8731)
                     /gene="mspA"
                     /locus_tag="O0293_000127"
     CDS             complement(8414..8731)
                     /gene="mspA"
                     /locus_tag="O0293_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane stabilizing protein MspA"
                     /protein_id="HCY0279040.1"
                     /translation="MQFYLILLAILYLIVSFISIFKMEVVFTRILRIIMGVLLLFVLA
                     LTTMSFPKENWWVFIVLLLLVGNVEVTGFKMLKKDLKGVNILNLMSLFIFVIYFILTI
                     VLF"
     gene            complement(8976..9140)
                     /locus_tag="O0293_000128"
     CDS             complement(8976..9140)
                     /locus_tag="O0293_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279041.1"
                     /translation="MSLENQLAELKYDYVRLQGDIEKRESLNLDTSALVRQLKDIENE
                     IRNVRAQMQD"
     gene            9273..10181
                     /locus_tag="O0293_000129"
     CDS             9273..10181
                     /locus_tag="O0293_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HCY0279042.1"
                     /translation="MTEQFTMIILLIALGYFLKRINFIKATDSQVIATLVLNVTLPSL
                     VIVNLNSAELKLSFSILPILMIIYGIVAKMIAVWFFRKYDNHMRGSVGMMTGAMNIGL
                     FAYPLIEVIWPKTGLIYFVMADIGGAFVMFGITYFVGSYFSEGSDQFDFKYLGKKLLQ
                     SVPLVTYIVMFILNISHIHLPHVAIDFFSILSHANMPLSMILLGVMLNFTLERKYIPA
                     TIKYLCLHYGLALVAGLLVYVLLPVSDDMIKTTLLVTWMFPVGVAIISYGIQFKYRTL
                     TFIGMTTNLTIIISIIILYVYQAVFV"
     gene            10352..10483
                     /locus_tag="O0293_000130"
     CDS             10352..10483
                     /locus_tag="O0293_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279043.1"
                     /translation="MYGAYLIYEYHQDFKSIANFKVLKRMINVMMCYCVLFLEPRIS"
     gene            complement(10600..11463)
                     /locus_tag="O0293_000131"
     CDS             complement(10600..11463)
                     /locus_tag="O0293_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein GlcU"
                     /protein_id="HCY0279044.1"
                     /translation="MQFLDFLIALLPALFWGSVVLINVFVGGGPYNQIRGTTLGALIV
                     GLGLLITGFAKFNNPTVIIVGLISGALWAFGQANQLKSISLIGVSNTMPVSTGMQLVG
                     TTLFSVIFLGEWSSMTQIIFGLIAMILLVTGVALTSLKAKNERQSDNPEFKKAMGILI
                     VSTVGYVGFVVLGDIFGVGGTDALFFQSVGMAIGGFILSMNHKTSLKSTALNLLPGVI
                     WGIGNLFMFYSQPKVGVATSFSLSQLLVIVSTLGGIFILGERKDRRQMTGIWAGIIII
                     VIAAIILGNLK"
     gene            complement(11644..12531)
                     /locus_tag="O0293_000132"
     CDS             complement(11644..12531)
                     /locus_tag="O0293_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0279045.1"
                     /translation="MSIVQLYDITQIKSFIEHSNYESASYLYKLPQQYNEIDVLITDA
                     IESPGVFSIKENDSIKAIILSFAYDKNKFKVIGPFVADNYVLSVDTFETLFKAMTSNQ
                     PDDAVFNFSFEEGIQQYKPLMKVIQASYNFTDYYIEARTRLEEDMHQPNIIPYHKGFY
                     RAFSKLHTTTFKYQAQSPQDIIDSLDDHHHLFLFVSEGLLKGYLYLEIDSQQSIAEIK
                     YFSSHVDYRLKGIAFELLAYALQYAFDNFDIRKVYFKIRNKNNKLIERFNGLGFHINY
                     EYIKFKFESRNVKDQTIPE"
     gene            12597..12692
                     /locus_tag="O0293_000133"
     CDS             12597..12692
                     /locus_tag="O0293_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279046.1"
                     /translation="MILTCYNTFNTSFAIIEFKSKISGIDEARSK"
     gene            12718..14853
                     /locus_tag="O0293_000134"
     CDS             12718..14853
                     /locus_tag="O0293_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCY0279047.1"
                     /translation="MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHL
                     VTNATPEQYDKNLKEWRLEDLPIIPKYMKTVVIGKTSKQFKTVKALILDNKVKDIIIA
                     TDAGREGELVARLILDKVGNKKPIRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAAL
                     ARSEADWIVGINATRALTTKYDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYFTLSL
                     TVKGFDFQLESNQRYTNKETLEQMVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQ
                     DMYRRYKIGPKETLNTLQSLYERHKVVTYPRTDSNYLTTDMVDTMKERIQATMATTYK
                     DQARPLMSKTFSSKMSIFNNQKVSDHHAIIPTEVRPVMSDLSNRELKLYDMIVERFLE
                     ALMPPHEYDAITVTLEVAGHTFVLKENVTTVLGFKSIRQGESITEMQQPFSEGDEVKI
                     SKTNIREHETTPPEYFNEGSLLKAMENPQNFIQLKDKKYAQTLKQTGGIGTVATRADI
                     IDKLFNMNAIESRDGKIKVTSKGKQILELAPEELTSPLLTAQWEEKLLLIERGKYQAK
                     TFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTECPTCGKFMIKVKTKNGQMLVCQDPS
                     CKTKKNVQRKTNARCPNCKKKLTLFGKGKEAVYRCVCGHSETQAHMDQRMKSKSSGKV
                     SRKEMKKYMNKNEGLDNNPFKDALKNLNL"
     gene            14966..16300
                     /locus_tag="O0293_000135"
     CDS             14966..16300
                     /locus_tag="O0293_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCY0279048.1"
                     /translation="MKNYFQFDKYGTNFKREILGGITTFLSMAYILAVNPQVLSLAGV
                     KGVSEDMKMDQGAIFVATALAAFVGSLFMGLIAKYPIALAPGMGLNAFFAFTVVLTMG
                     IPWQVGLTGVLFSGIFFAILTVTGFREVIINAIPYQMKMAVSAGIGLFITFVGLQSSG
                     IIVKNESTLVTLGHLTDPPVLLAIFGIVITVILYAIKLPGSIFIGMIITAIVGMFTGL
                     IQMPSGIVGKIPSIEPTFGAAFEAFKDPSQLLTIQFLIVILTFLFIDFFDTAGTLVAV
                     ATQAGIMKDNKLPRAGRALFSDSLATIVGSVFGTTTTTSYIESTSGVAVGARTGFASI
                     VTGCCFLLALFFSPLIAVVTSAVTTPALVVVGVLMAANFAEINWKSFEVAVPAFITII
                     MMPLSYSIATGIACGFIFYPITMLISKKHKEVHPIMYVLMVLFILYFIFVHG"
     gene            complement(16492..16881)
                     /locus_tag="O0293_000136"
     CDS             complement(16492..16881)
                     /locus_tag="O0293_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279049.1"
                     /translation="MKNGPKLSLALIGIFLILCEFFYGIPFLGATFILSFGWQPLLFN
                     ALLYLILTIILLVNRQNAIRPIAIIPIFGIVGSFLAIIPFLGILIHWILFFLMILFVL
                     VVLSAPTYIPNKNARVIYTQYKDGHRE"
     gene            17238..17546
                     /gene="rpsJ"
                     /locus_tag="O0293_000137"
     CDS             17238..17546
                     /gene="rpsJ"
                     /locus_tag="O0293_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCY0279050.1"
                     /translation="MAKQKIRIRLKAYDHRVIDQSAEKIVETAKRSGADVSGPIPLPT
                     EKSVYTIIRAVHKYKDSREQFEQRTHKRLIDIVNPTPKTVDALMGLNLPSGVDIEIKL
                     "
     gene            17574..18236
                     /gene="rplC"
                     /locus_tag="O0293_000138"
     CDS             17574..18236
                     /gene="rplC"
                     /locus_tag="O0293_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCY0279051.1"
                     /translation="MTKGILGRKIGMTQVFGENGELIPVTVVEAKENVVLQKKTVEVD
                     GYNAIQVGFEDKKAYKKDAKSNKYANKPAEGHAKKADAAPKRFIREFRNVDVDAYEVG
                     QEVSVDTFVAGDVIDVTGVSKGKGFQGAIKRHGQSRGPMSHGSHFHRAPGSVGMASDA
                     SRVFKGQKMPGRMGGNTVTVQNLEVVQVDTENKVILVKGNVPGPKKGLVEIRTSIKKG
                     NK"
     gene            18263..18886
                     /gene="rplD"
                     /locus_tag="O0293_000139"
     CDS             18263..18886
                     /gene="rplD"
                     /locus_tag="O0293_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCY0279052.1"
                     /translation="MANYDVLKLDGTKSGSIELSDAVFGIEPNNSVLFEAINLQRASL
                     RQGTHAVKNRSAVSGGGRKPWKQKGTGRARQGTIRAPQWRGGGIVFGPTPRSYAYKMP
                     KKMRRLALRSALSFKAQENGLTVVDAFNFEAPKTKEFKNVLSTLEQPKKVLVVTENED
                     VNVELSARNIPGVQVTTAQGLNVLDITNADSLVITEAAAKKVEEVLG"
     gene            18886..19161
                     /gene="rplW"
                     /locus_tag="O0293_000140"
     CDS             18886..19161
                     /gene="rplW"
                     /locus_tag="O0293_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002506966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCY0279053.1"
                     /translation="MEARDILKRPVITEKSSEAMAEDKYTFDVDTRVNKTQVKMAVEE
                     IFNVKVASVNIMNYKPKKKRMGRYQGYTNKRRKAIVTLKEGSIDLFN"
     gene            19194..20027
                     /gene="rplB"
                     /locus_tag="O0293_000141"
     CDS             19194..20027
                     /gene="rplB"
                     /locus_tag="O0293_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCY0279054.1"
                     /translation="MAIKKYKPITNGRRNMTSLDFAEITKTTPEKSLLKPLPKKAGRN
                     NQGKLTVRHHGGGHKRQYRVIDFKRNKDGINAKVDSIQYDPNRSANIALVVYADGEKR
                     YIIAPKGLEVGQIVESGAEADIKVGNALPLQNIPVGTVVHNIELKPGKGGQIARSAGA
                     SAQVLGKEGKYVLIRLRSGEVRMILSTCRATIGQVGNLQHELVNVGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGRAPIGRPSPMSPWGKPTLGKKTRRGKKSSDKLIVRGRKK
                     K"
     gene            20094..20372
                     /gene="rpsS"
                     /locus_tag="O0293_000142"
     CDS             20094..20372
                     /gene="rpsS"
                     /locus_tag="O0293_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCY0279055.1"
                     /translation="MARSIKKGPFVDEHLMKKVEAQEGSEKKQVIKTWSRRSTIFPNF
                     IGHTFAVYDGRKHVPVYVTEDMVGHKLGEFAPTRTFKGHVADDKKTRR"
     gene            20401..20754
                     /gene="rplV"
                     /locus_tag="O0293_000143"
     CDS             20401..20754
                     /gene="rplV"
                     /locus_tag="O0293_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCY0279056.1"
                     /translation="MEAKAVARTIRIAPRKVRLVLDLIRGKNAAEAIAILKLTNKASS
                     PVIEKVLMSALANAEHNYDMNTDELVVKEAYANEGPTLKRFRPRAQGRASAINKRTSH
                     ITIVVSDGKEEAKEA"
     gene            20778..21431
                     /gene="rpsC"
                     /locus_tag="O0293_000144"
     CDS             20778..21431
                     /gene="rpsC"
                     /locus_tag="O0293_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCY0279057.1"
                     /translation="MGQKINPIGLRVGIIRDWEAKWYAEKDFASLLHEDLKIRKFIDN
                     ELKEASVSHVEIERAANRINIAIHTGKPGMVIGKGGSEIEKLRNKLNALTDKKVHINV
                     IEIKKVDLDARLVAENIARQLENRASFRRVQKQAITRAMKLGAKGIKTQVSGRLGGAD
                     IARAEQYSEGTVPLHTLRADIDYAHAEADTTYGKLGVKVWIYRGEVLPTKNTSGGGK"
     gene            21434..21868
                     /gene="rplP"
                     /locus_tag="O0293_000145"
     CDS             21434..21868
                     /gene="rplP"
                     /locus_tag="O0293_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCY0279058.1"
                     /translation="MLLPKRVKYRRQHRPKTTGRSKGGNYVTFGEFGLQATTTSWITS
                     RQIESARIAMTRYMKRGGKVWIKIFPHTPYTKKPLEVRMGAGKGAVEGWIAVVKPGRI
                     LFEVAGVSEEVAREALRLASHKLPVKTKFVKREELGGETNES"
     gene            21858..22067
                     /gene="rpmC"
                     /locus_tag="O0293_000146"
     CDS             21858..22067
                     /gene="rpmC"
                     /locus_tag="O0293_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCY0279059.1"
                     /translation="MKAKEIRDLTTSEIEEQIKSSKEELFNLRFQLATGQLEETARIR
                     TVRKTIARLKTVAREREIEQSKANQ"
     gene            22091..22354
                     /gene="rpsQ"
                     /locus_tag="O0293_000147"
     CDS             22091..22354
                     /gene="rpsQ"
                     /locus_tag="O0293_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCY0279060.1"
                     /translation="MSERNDRKVYVGKVVSDKMDKTITVLVETYKTHKLYGKRVKYSK
                     KYKTHDENNSAKLGDIVKIQETRPLSATKRFRLVEIVEESVII"
     gene            22386..22754
                     /gene="rplN"
                     /locus_tag="O0293_000148"
     CDS             22386..22754
                     /gene="rplN"
                     /locus_tag="O0293_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCY0279061.1"
                     /translation="MIQQETRLKVADNSGAREVLTIKVLGGSGRKTANIGDVIVCTVK
                     NATPGGVVKKGDVVKAVIVRTKSGVRRNDGSYIKFDENACVIIRDDKGPRGTRIFGPV
                     ARELREGNFMKIVSLAPEVL"
     gene            22790..23107
                     /gene="rplX"
                     /locus_tag="O0293_000149"
     CDS             22790..23107
                     /gene="rplX"
                     /locus_tag="O0293_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCY0279062.1"
                     /translation="MHIKKGDNVKVIAGKDKGKEGKVIATLPKKDRVVVEGVNIMKKH
                     QKPTQLNPEGGILETEAAIHVSNVQLLDPKTNEPTRVGYKFVDGKKVRIAKKSGEEIK
                     SNN"
     gene            23134..23673
                     /gene="rplE"
                     /locus_tag="O0293_000150"
     CDS             23134..23673
                     /gene="rplE"
                     /locus_tag="O0293_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCY0279063.1"
                     /translation="MNRLKEKFNTEVTENLMKKFNYSSVMEVPKIDKIVVNMGVGDAV
                     QNSKVLDNAVEELELITGQKPLVTKAKKSIATFRLREGMPIGAKVTLRGERMYEFLDK
                     LISVSLPRVRDFQGVSKKAFDGRGNYTLGVKEQLIFPEIDYDKVSKVRGMDIVIVTTA
                     NTDEEARELLANFGMPFRK"
     gene            23696..23881
                     /locus_tag="O0293_000151"
     CDS             23696..23881
                     /locus_tag="O0293_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HCY0279064.1"
                     /translation="MAKTSMVAKQQKKQKYAVREYTRCERCGRPHSVYRKFKLCRICF
                     RELAYKGQIPGVRKASW"
     gene            23913..24311
                     /gene="rpsH"
                     /locus_tag="O0293_000152"
     CDS             23913..24311
                     /gene="rpsH"
                     /locus_tag="O0293_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCY0279065.1"
                     /translation="MTMTDPIADMLTRVRNANMVRHEKLELPASNIKKEIAEILKSEG
                     FIKNVEYVEDDKQGVLRLFLKYGQNDERVITGLKRISKPGLRVYAKASEMPKVLNGLG
                     IALVSTSEGVITDKEARKRNVGGEIIAYVW"
     gene            24336..24872
                     /gene="rplF"
                     /locus_tag="O0293_000153"
     CDS             24336..24872
                     /gene="rplF"
                     /locus_tag="O0293_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCY0279066.1"
                     /translation="MSRVGKKIIDIPSDVTVTFDGNHVTVKGPKGELSRTLNERMTFK
                     QEENTIEVVRPSDSKEDRTNHGTTRALLNNMVQGVSQGYVKVLELVGVGYRAQMQGKD
                     LILNVGYSHPVEIKAEENITFSVEKNTVVKVEGISKEQVGALASNIRSVRPPEPYKGK
                     GIRYQGEYVRRKEGKTGK"
     gene            24903..25262
                     /gene="rplR"
                     /locus_tag="O0293_000154"
     CDS             24903..25262
                     /gene="rplR"
                     /locus_tag="O0293_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCY0279067.1"
                     /translation="MISKIDKNKVRLKRHARVRTNLSGTAEKPRLNVYRSNKHIYAQI
                     IDDNKGVTLAQASSKDSDIATTATKVELATKVGEAIAKKAADKGIKEIVFDRGGYLYH
                     GRVKALAEAARESGLEF"
     gene            25283..25783
                     /gene="rpsE"
                     /locus_tag="O0293_000155"
     CDS             25283..25783
                     /gene="rpsE"
                     /locus_tag="O0293_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCY0279068.1"
                     /translation="MARREEETKEFEERVVTINRVAKVVKGGRRFRFTALVVVGDKNG
                     RVGFGTGKAQEVPEAIKKAVEAAKKDLVVVPRVEGTTPHTITGRYGSGSVFMKPAAPG
                     TGVIAGGPVRAVLELAGITDILSKSLGSNTPINMVRATIDGLQNLKNAEDVAKLRGKT
                     VEELYN"
     gene            25800..25979
                     /gene="rpmD"
                     /locus_tag="O0293_000156"
     CDS             25800..25979
                     /gene="rpmD"
                     /locus_tag="O0293_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCY0279069.1"
                     /translation="MAKLQITLTRSVIGRPETQRKTVEALGLKKTNSSVVVEDNPAIR
                     GQINKVKHLVTVEEK"
     gene            25996..26436
                     /gene="rplO"
                     /locus_tag="O0293_000157"
     CDS             25996..26436
                     /gene="rplO"
                     /locus_tag="O0293_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCY0279070.1"
                     /translation="MKLHELKPAEGSRKERNRVGRGVATGNGKTSGRGHKGQKARSGG
                     GVRPGFEGGQLPLFRRLPKRGFTNINRKEYAIVNLDQLNKFEDGTEVTPALLVESGVV
                     KNEKSGIKILGNGSLDKKLTVKAHKFSASAAEAIDAKGGAHEVI"
     gene            26436..27728
                     /gene="secY"
                     /locus_tag="O0293_000158"
     CDS             26436..27728
                     /gene="secY"
                     /locus_tag="O0293_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCY0279071.1"
                     /translation="MIQTLVNFFRTKEVRNKIFFTLAMLVIFKIGTYIPAPGVNPAAF
                     DNPQGSQGATELLNTFGGGALKRFSIFAMGIVPYITASIVMQLLQMDIVPKFSEWAKQ
                     GEVGRRKLNNVTRYLAISLAFIQSIGMAFQFNNYLKGALIINQSIMSYLLIALVLTAG
                     TAFLIWLGDQITQFGVGNGISIIIFAGILSTLPASLIQFGQTAFVGQEDTSLAWLKVL
                     GLLVSLILLTVGAIYVLEAVRKIPIQYAKKQTAQRLGSQATYLPLKVNSAGVIPVIFA
                     MAFFLLPRTLTLFYPDKEWAQNIANAANPSSNVGMVVYIVLIILFTYFYAFVQVNPEK
                     MADNLKKQGSYVPGIRPGEQTKKYITKVLYRLTFVGSIFLAVISILPILATKFMGLPQ
                     SIQIGGTSLLIVIGVAIETMKSLEAQVSQKEYKGFGGR"
     gene            27745..28392
                     /locus_tag="O0293_000159"
     CDS             27745..28392
                     /locus_tag="O0293_000159"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCY0279072.1"
                     /translation="MNIILMGLPGAGKGTQASEIVKKFPIPHISTGDMFRKAIKEETE
                     LGKEAKSYMDRGELVPDEVTVGIVKERISEDDAKKGFLLDGFPRTIEQAEALNNIMSE
                     LDRNIDAVINIEVPEEELMNRLTGRRICESCGTTYHLVFNPPKVEGICDIDGGKLYQR
                     EDDNPETVANRLSVNIKQSKPILDFYDQKGVLKNIDGSKDISDVTKDVIDILDHL"
     gene            28585..28803
                     /gene="infA"
                     /locus_tag="O0293_000160"
     CDS             28585..28803
                     /gene="infA"
                     /locus_tag="O0293_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCY0279073.1"
                     /translation="MAKQDVIELEGTVLDTLPNAMFKVELENGHEILAHVSGKIRMNY
                     IRILPGDKVTVEMSPYDLTRGRITYRYK"
     gene            28835..28948
                     /gene="rpmJ"
                     /locus_tag="O0293_000161"
     CDS             28835..28948
                     /gene="rpmJ"
                     /locus_tag="O0293_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCY0279074.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQRQG"
     gene            28971..29336
                     /gene="rpsM"
                     /locus_tag="O0293_000162"
     CDS             28971..29336
                     /gene="rpsM"
                     /locus_tag="O0293_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCY0279075.1"
                     /translation="MARIAGVDIPREKRVVISLTYIYGIGTSTAQKILEEANVSADTR
                     VKDLTDDELGRIREVVDGYKVEGDLRRETNLNIKRLMEISSYRGIRHRRGLPVRGQKT
                     KNNARTRKGPVKTVANKKK"
     gene            29360..29749
                     /gene="rpsK"
                     /locus_tag="O0293_000163"
     CDS             29360..29749
                     /gene="rpsK"
                     /locus_tag="O0293_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCY0279076.1"
                     /translation="MARKQVSRKRRVKKNIENGVAHIRSTFNNTIVTITDEFGNALSW
                     SSAGALGFKGSKKSTPFAAQMASETASKSAMEHGLKTVEVTVKGPGPGRESAIRALQS
                     AGLEVTAIRDVTPVPHNGCRPPKRRRV"
     gene            29824..30768
                     /locus_tag="O0293_000164"
     CDS             29824..30768
                     /locus_tag="O0293_000164"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCY0279077.1"
                     /translation="MIEIEKPRIETIEISEDAKFGKFVVEPLERGYGTTLGNSLRRIL
                     LSSLPGAAVKYIEIEGVLHEFSAVDNVVEDVSTIIMNIKQLALKIYSEEDKTLEIDVR
                     DEGEVTASDITHDSDVEILNPELKIATVSKGGHLKIRLVANKGRGYALAEQNNTSDLP
                     IGVIPVDSLYSPVERVNYTVENTRVGQSSDFDKLTLDVWTNGSITPQESVSLAAKIMT
                     EHLNIFVGLTDEAQNAEIMIEKEEDQKEKVLEMSIEELDLSVRSYNCLKRAGINSVQE
                     LADKSEADMMKVRNLGRKSLEEVKYKLEDLGLGLRKED"
     gene            30785..31153
                     /gene="rplQ"
                     /locus_tag="O0293_000165"
     CDS             30785..31153
                     /gene="rplQ"
                     /locus_tag="O0293_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002450507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCY0279078.1"
                     /translation="MGYRKLGRTSDQRKAMLRDLATSLIISERIETTEARAKEVRSVV
                     EKLITLGKKGDLASRRNAAKTLRNVEILNEDETTQTALQKLFGEIAERYTERQGGYTR
                     ILKQGPRRGDGAESVIIELV"
     gene            31691..32500
                     /locus_tag="O0293_000166"
     CDS             31691..32500
                     /locus_tag="O0293_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HCY0279079.1"
                     /translation="MEDKNSVIVFKNVSFQYQSDASFTLKDVSFNIPKGQWTSIVGHN
                     GSGKSTIAKLMIGIEKVKSGEIFYNNQAITDDNFEKLRKDIGIVFQNPDNQFVGSIVK
                     YDVAFGLENHAVPYDEMHRRVSEALKQVDMLERADYEPNALSGGQKQRVAIASVLALN
                     PSVIILDEATSMLDPDARQNLLDLVRKVKSEHNITIISITHDLSEAMEADHVIVMNKG
                     TVYKEGTATEIFDNAEELTRIGLDLPFPIKINQMLGHQTSFLTYEGLVDQL"
     gene            32497..33357
                     /locus_tag="O0293_000167"
     CDS             32497..33357
                     /locus_tag="O0293_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HCY0279080.1"
                     /translation="MTIRFDNVSYTYQKGTPYQHQAIHDVNTEFEQGKYYAIVGQTGS
                     GKSTLIQNINALLKPTTGTVTVDDITITHKTKDKYIRPVRKRIGMVFQFPESQLFEDT
                     VEREMIFGPKNFKMNLDEAKNYAHRLLMDLGFSRDVMSQSPFQMSGGQMRKIAIVSIL
                     AMNPDIIVVDEPTAGLDPQSKRQVMRLLKSLQTDENKAIILISHDMNEVARYADEVIV
                     MKEGSIVSQTSPKELFKDKKKLADWHIGLPEIVQLQYDFEQKYQTKLKDIALTEEAFV
                     SLYKEWQHEK"
     gene            33347..34153
                     /locus_tag="O0293_000168"
     CDS             33347..34153
                     /locus_tag="O0293_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCY0279081.1"
                     /translation="MKNKLIIGRYLPINSFVHHLDPRAKLMFVFLFIILIFFCHSPLT
                     YLWVFALILFIMRLAKIQLWFLIKGLTPIFFFLIFTLMMHIFLTKGGYVLVEWHGITI
                     ETNGILEGLYISLRLIGIVMIATIMTLSTSPIDLTDAFERLLAPLKMFKLPVHQLSMI
                     MSIALRFIPTLMDELDKIILAQKSRGSEISSGNIATRIKSFIPLLVPLFISAFQRAEE
                     LAVAMEVRGYDANVKRTSYRQLKWQLRDTISLTMIIPIAIILFVLKYSGV"
     gene            34158..34961
                     /gene="truA"
                     /locus_tag="O0293_000169"
     CDS             34158..34961
                     /gene="truA"
                     /locus_tag="O0293_000169"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCY0279082.1"
                     /translation="MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRMHKRHVRI
                     HPSSRTDRGVHAIQQYFHFDTELNIPMSQWQYAMNRTLPDDIYVNNVVTVDDDFHCRY
                     DCVGKRYRYKVYQAQHRDPFQSGLKTFIPETLDLGKMNRAAQQFIGTHDFTGFCSQKT
                     EVESKVRTLYQSEIVKTDDGFDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEVSDVPKL
                     LESKNRKNVPFTAPAEGLYLEKIYLDENELLKDFGNDIKIHRKKSLQND"
     gene            35201..35638
                     /gene="rplM"
                     /locus_tag="O0293_000170"
     CDS             35201..35638
                     /gene="rplM"
                     /locus_tag="O0293_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCY0279083.1"
                     /translation="MRQTFMANESNIERKWYVIDAEGQTLGRLSSEVASILRGKNKVT
                     YTPHVDTGDYVIVINASKIEFTGNKETDKVYYRHSNHPGGIKSITAGELRRTNPERLI
                     ENSIKGMLPSTRLGEKQGKKLFVYGGAEHPHAAQQPENYELRG"
     gene            35652..36050
                     /gene="rpsI"
                     /locus_tag="O0293_000171"
     CDS             35652..36050
                     /gene="rpsI"
                     /locus_tag="O0293_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCY0279084.1"
                     /translation="MTLAQVEYRGTGRRKNSVARVRLVPGEGNITVNNRDVREYLPFE
                     SLILDLNQPFDVTETKGNYDVLVNVHGGGFTGQAQAIRHGIARALLEADPEYRGSLKR
                     AGLLTRDPRMKERKKPGLKAARRSPQFSKR"
     gene            36595..37692
                     /locus_tag="O0293_000172"
     CDS             36595..37692
                     /locus_tag="O0293_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCY0279085.1"
                     /translation="MRGDQRTQGEKSRKEGGKIFGSGSRAPIAISILVKDGSYNHDIY
                     YNDIGEYLTREQKLDTLMKHQSIVNLKSLNVLPDKNNDWINQRDINYENYLPMYDSKD
                     IENSIYLDQFNGVNSARDNWVTNFSNEKALVNAKLLVDNYNSEIDRLIDILDSRERIN
                     LVNKDETFISWTRGLTQKFSKGKNISINPERIVKFMHRPFTKKWIVYDKNIMEMPSRY
                     YNIMENTGQVIYIQGQGMNKEFSAMITDILPNFQFIGNGKGFATYKGKDSLRLVDNIS
                     NSFKKKINLNSEEIVYYIYAILHHKYYVNKYSSDLSKGFPRIPILKDVYGFVEIGREL
                     VELHLNYEKQLNWDGVEIIYNNMNPNYKVEK"
     gene            37713..37982
                     /locus_tag="O0293_000173"
     CDS             37713..37982
                     /locus_tag="O0293_000173"
                     /inference="COORDINATES: protein motif:HMM:NF037202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCY0279086.1"
                     /translation="MDTVIYNTDITVTNIPNKVYNYIVNGKPAIEWIIDQYNVSIDKK
                     SGILDDPNEFSEDPNYILNLLLSVITVSMKTLGLIDKLPDFEVLD"
     gene            38043..38786
                     /locus_tag="O0293_000174"
     CDS             38043..38786
                     /locus_tag="O0293_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279087.1"
                     /translation="MDLLQIEKKGNLTKTVQLMHDEKGSASGESKVNMEFGKQSAVKQ
                     IFDSLLGMSASASASAGINGNITGERVAKTILENTLIYDFLDTVEFRKKKTIIDISEG
                     FKLNIQQDTMTYFATITPIASMFEGSKHLDEEELSFDVSKMNEAIRSIKGYFELIGTR
                     GNENRVFRFNIESFKNNYRIQDLKFMNLTLYSVLVGEISKENLNYDKEFDLNQGAKPS
                     GIGKFKGIRSEATKEDKYQVYDVILAGVK"
     gene            38787..>39093
                     /locus_tag="O0293_000175"
     CDS             38787..>39093
                     /locus_tag="O0293_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCY0279088.1"
                     /translation="MEIHVYTGPLCGFNEYINSEVKEYHRFMELIREYNIIVRASDTF
                     GTHDVDGIFDTEIENLVIFSNDFASVTTHVITNFINIVTLGRNIKKIYIQNPPKRVFQ
                     "
BASE COUNT        14276 a         5845 c         7375 g        11597 t
ORIGIN      
        1 agaaacatag gaggcatcat attatgagta ataaagttca acgttttata gaagcagaaa
       61 gggagttaag tcagttaaag cactggttaa aaacaacaca taagatttca attgaagaat
      121 ttgtagtcct ttttaaagtg tatgaagctg aaaagattag cggtaaagaa ttgagggata
      181 cattacattt tgaaatgcta tgggatacaa gtaaaatcga tgtgattatc cgtaaaatct
      241 ataaaaaaga gcttatttct aaattgcgtt ctgaaacgga tgaaagacaa gtattctatt
      301 tctatagtac ttctcaaaag aaattgttag ataaaattac taaagaaata gaagtgttaa
      361 gcgttacaaa ctaaaaactt aaaaagcatg ccaatcagta catcataatt gcgtcttggg
      421 gacagacaaa tgatgaatag agattggcat gctttttatt tttgaatata aatatttagt
      481 tcatggcatt tctagttaca tgacgtccat gaattaagaa gtaaacaagc atagtaatga
      541 ttgctaaagc ggccataaag ccgaagattt cactatatga aaacatatga gtaaataacc
      601 caaggaatga tggaccgaag ccgacacctg catctagacc aacgtaaaaa gtagatgtcg
      661 cgataccata tttaatcggg ggtgagactt ttatcgcaat agattgcatt gcagatgata
      721 aatttccata ccctaaacct aggcaagcac cagcaagtaa tattaaccag ctttgatagc
      781 ttgaaattaa gcatacaaat gaaaggaaaa gcatgataaa tgctgggtag acaataatat
      841 tttcattttt atcatccatc aatctaccag caataggtct agtaattaac gatgctatag
      901 catagcaaat aaagaaatag cttgctgcag tgactaggtg tcgctctaaa gcaaatgctt
      961 gtaaataagt taggatggac gcataggtaa cgccaattaa aagcataatt acagcaacag
     1021 gaatggcctc ttttgcaata aattgatgaa tactaaatct tggtttatca atgacattag
     1081 tttcagtttt gttatttgtt acttcgaaat caacttttat aaataatgag ataatgagtc
     1141 cgagtatgcc taatatgaca caaataataa acagtaagtc aattgcgtat tttgtaataa
     1201 gtaacatgcc tagaaatggg ccaatcgctg tacctaatac taaacttaag gaaaataaac
     1261 tgatgccttc actttttcta ttaacagggg taacgtatgc cgcaatagta cctgttgcag
     1321 ttgtcacaac tgcagttgcg ataccgttta tgagacgtac aaagattaaa aaagctaaag
     1381 atccatcaat aaaataaagt aattgcgtga taattaaagc aattaaacca ataaataata
     1441 atcgtttagg tccgatttga tttacaaatt tacctgtagc aaatcgacca attaaagacc
     1501 cgacaataaa tagtcctaca actaatccgg ctaatgaatc ggaagcgtcg aaagcttgct
     1561 tactatagcc agcaataact acaagtaaca aatacatgca taggtatacg ataaagttga
     1621 ctaaaaagtt aattgtaaaa cttttggtga aaataggtga tttatttaat gagccttgat
     1681 tagtcattgt gtgcctccct taataatatt tcatgaattt gattcatcac atttatggtt
     1741 tcttgcattt gatcttgcga aatgtttgtt ttggcaacta ctgcttgttg gagcttttgt
     1801 acatcaagac aaacaatctc atataattca tgccctttag gtgttaaagt taaaaacttt
     1861 tgtcgtttat cctctaaact gttttctact gttataaggt cattttctat tagagctttt
     1921 aaagtttttc ttgcagtagg cttttcaatt gcacgtctat gtgaaatttc aatgagagta
     1981 gtgggctgat gtttagcgat atcgcgtaag attaaccatt ggccataata tatattgtgt
     2041 ttttctaaaa tcggctcggc taattttaaa taggggcgat atattgttat aaaactattg
     2101 aaaaattctt gtgatagcat agtgacatct cctaagacaa aatagttagc ttagctaacc
     2161 tttttacaac aatagtaatt ataaaacggg agcaattaga aatcaatata taattattaa
     2221 gagcaaaaat aattatactt tgttaaaata agcgtaatta catgtaaata gggggatact
     2281 aatgatattg aaatttgatc acatcattca ttatatagat cagttagatc ggtttagttt
     2341 tccaggagat gttataaaat tacattcagg tgggtatcat cataaatatg gaacattcaa
     2401 taaattaggt tatatcaatg aaaattatat tgagctacta gatgtagaaa ataatgaaaa
     2461 gttgaaaaag atggcaaaaa cgatagaagg cggagtcgct tttgctactc aaattgttca
     2521 agagaagtat gagcaaggct ttaaaaatat ttgtttgcgt acaaatgata tagaggcagt
     2581 taaaaataaa ctacaaagtg agcaggttga agtagtaggg ccgattcaaa tggaaagaga
     2641 tacacataaa gatggtaagg taaagtggca attgctttat ataatgaatc aggatgatga
     2701 tgaaattaag ccaccatttt ttattcaatg ggaagaaagt gattccatgc gtactaaaaa
     2761 attgcaaaaa tattttcaaa aacaattttc aattgaaact gttattgtga aaagtaaaaa
     2821 ccgatcacaa acagtatcga attggttgaa atggtttgat atggacattg tagaagagaa
     2881 tgaccattac acagatttga ttttaaaaaa tgatgatatt tattttagaa ttgaagatgg
     2941 taaagtttca aaatatcatt cggttatcat aaaagacgca caagcaactt caccatattc
     3001 aatttttatc agaggtgcta tttatcgctt tgaaccatta gtataaatat acgtaagtgc
     3061 tatgagcgag aatgcccata tgaataatga caagcacaat ggaaagaatc gttaatatat
     3121 tatttaatcg tgatgactta attaaaatga aaaagattga taatataaat gtgaaaaaga
     3181 taagtataac ccgtaaacta aagtaattca cggtgagagg ttgactcaat gtcataatga
     3241 ttgcaacgat gttcataatt ataaatagac ttaaaataat tgttctcata tcaaacacct
     3301 cattgttaga ttattgacat tataacaggg gtaattgtat atgaacatta atgtggttgc
     3361 ttgaggaaaa atttattcat tgaagtcaag ttggttcatt ttagaaatga atatcgtgtt
     3421 agatgatgaa agtatattga agtataggta actagttgaa aagtattaat tgtacgataa
     3481 cattaaattt aacacgaaac atagatataa aatgattcac aattaaaatg ggtaaatttg
     3541 aacttgctaa actattaatt ggagcatgga catttcaaaa ataagagttc aaatcttaca
     3601 caagctctga atcgacacta taagatacaa actgtataat taaaggtatt gttaaataga
     3661 aggagatatc ataaatcatg gaaaagatgc atatcactaa tcaggaacat gacgcatttg
     3721 ttaaatccca cccaaatgga gatttattac aattaacgaa atgggcagaa acaaagaaat
     3781 taactggatg gtacgcgcga agaatcgctg taggtcgtga cggtgaagtt cagggtgttg
     3841 cgcagttact ttttaaaaaa gtacctaaat taccttatac gctatgttat atttcgcgtg
     3901 gttttgttgt tgattatagt aataaagaag cgttaaatgc attgttagac agtgcaaaag
     3961 aaattgctaa agctgagaaa gcgtatgcaa ttaaaatcga tcctgatgtt gaagttgata
     4021 aaggtacaga tgctttgcaa aatttgaaag cgcttggttt taaacataaa ggatttaaag
     4081 aaggtttatc aaaagactac atccaaccac gtatgactat gattacacca attgataaaa
     4141 atgatgatga gttattaaat agttttgaac gccgaaatcg ttcaaaagtg cgcttggctt
     4201 taaagcgagg tacgacagta gaacgatctg atagagaagg tttaaaaaca tttgctgagt
     4261 taatgaaaat cactggggaa cgcgatggct tcttaacgcg tgatattagt tactttgaaa
     4321 atatttatga tgcgttgcat gaagatggag atgctgaact atttttagta aagttggatc
     4381 caaaagaaaa tatagcgaaa gtaaatcaag aattgaatga acttcatgcc gaaattgcta
     4441 aatggcagca gaagatgaaa acatctgaaa agcaagctaa aaaagcgcaa aatatgatta
     4501 atgatgcgca aaataaaatt gctaaaaatg aagatttaaa acgagaccta gaagctttag
     4561 aaaaggaaca tcctgaaggt atttatcttt ctggtgcact attaatgttt gctggctcaa
     4621 aatcatatta cttatatggt gcgtcttcta atgaatttag agatttttta ccaaatcatc
     4681 atatgcagta tacgatgatg aagtatgcac gtgaacatgg tgcaacaact tacgatttcg
     4741 gtggtacaga taatgatcca gataaagact cagaacatta tggattatgg gcatttaaaa
     4801 aagtgtgggg aacatactta agtgaaaaga ttggtgaatt tgattatgta ttgaatcagc
     4861 cattgtacca attaattgag caagttaaac cgcgtttaac aaaagctaaa attaaaatat
     4921 ctcgtaaatt aaaacgaaaa tagattaacg actgaaatct gaacgctcat aagactgtca
     4981 tttgcgttca gattttttta cacaatatag aatggttgag taaaatattt ttgaatatag
     5041 tgaaagaggg ggaagtactg tgataaaaaa gctattacaa ttttctttag ggaataagtt
     5101 tgctatcttt ttaatggttg ttttagttgt cttgggcggt gtatatgcga gtgctaaatt
     5161 gaaattagaa ttactaccaa atgtacaaaa tccagttatt tcagttacaa caacaatgcc
     5221 gggtgcaacg ccacaaagta cccaagatga aataagtagt aaaattgaca atcaagtaag
     5281 atcattggca tatgtgaaaa atgttaaaac gcaatccata caaaatgctt caattgtaac
     5341 agttgaatat gaaaataata cagatatgga taaagcagaa gaacagctta aaaaagaaat
     5401 cgataaaatt aaatttaaag atgaagttgg tcaaccagaa ttaagacgta attcgatgga
     5461 tgcttttccg gttttagcat attcattttc aaataaagag aatgacttga aaaaagtaac
     5521 gaaagtactg aatgaacaat taataccaaa attgcaaacg gtagatggtg tgcaaaatgc
     5581 gcaattaaat gggcagacga accgtgaaat cacccttaaa tttaagcaaa atgaacttga
     5641 aaaatatggg ttgactgctg atgatgtaga aaactatcta aaaacggcaa caagaacaac
     5701 gccacttgga ttgttccaat ttggtgataa agataaatca attgttgttg atggtcaata
     5761 tcaatctgtt gatgctttta aaaacataaa tattccatta acgttggcag gaggacaagg
     5821 gcaatctcaa tcccaaagtg acaataaaca aaattcagcc atgtcagacg ttaatcaggc
     5881 atcaccacag caaaattcaa aagcgtcagc accaaataat ataagtggta tgccgacagc
     5941 gaaactagga gatttagctg atattacagt tggtgatgtg cgtacttcta tttctaaaac
     6001 gaatggaaag gatgcggtta atctacaaat aactaaagct caagatgcca atacagttca
     6061 agtagccaaa gatgtacaac gtaaaattga tacattcgtt gatgaaaata aagattttaa
     6121 tgtcacaaaa acaatggata ctgcaaagcc tgttgagaaa tcactttata cgatggttga
     6181 aaaagcatca ttaggtacaa tcgtggcaat tatagttatt ttgctgtttt taagaaacat
     6241 tcgtacgacg gcaatttcta ttatatcgat tccgttatca cttcttatgg cgcttattgc
     6301 tctgaaattg agtgatgttt cattgaatat actaacgtta ggtgcattaa cagtagcgat
     6361 tggacgtgtg atagacgatt cgattgtagt tgttgaaaat atttatcgac gcttaacaga
     6421 ttcagaagaa caactaaaag gtgaaaattt aattatcagt gcgacaactg aagtatttaa
     6481 accaataatg tcatcgacac tagttactat tatcgtcttc ttaccacttg tgtttgtatc
     6541 aggttcagta ggcgaaatgt ttagaccttt tgcattggct attgcattta gtttattagc
     6601 atcgttatta gtgtcaatta cactcgttcc agcgttggca gctacactat ttaaaaaagg
     6661 cgttaaacgt cgtaataaac aacatcaaga aggattaggt gttgttagta caacttataa
     6721 aaaagtatta cattggtcat taaatcataa gtggattgta attatattaa gtacattaat
     6781 tttggttgca actattgtat ttggaggacc gagactaggc actagcttta tttcagcagg
     6841 tgacgataaa tttttagcta ttacttatac accgaagcct ggtgaaacgg agcaagcagt
     6901 gttgaatcat gcgaaagatg ttgaaaaata tttaaaacag aaaaagcatg taaaaacaat
     6961 tcaatactca gttggcggta gtagtccagt agatccaacg ggtagtacaa atagtatggc
     7021 aatcatggtt gaatatgata atgacacgcc taattttgat gtagaagcgg ataaggttat
     7081 taaacatgca gatggcttta aacatcctgg agagtggaaa aatcaagatt taggaacagg
     7141 tgcaggtaat aaatctgtag aggttactgt aaaaggtcca tcaatggatg ccataaaatc
     7201 aactgtaaaa gatattgaac agaaaatgaa acaggttaaa ggactagcca atgtcaaatc
     7261 tgatttatcg caaacatatg atcagtatga aattaaagtc gatcaaaata aagcggcaga
     7321 aaatggtatt tctgcaagtc aacttgcaat gcacttgaat gaaaacttac cagaaaaaac
     7381 agttacgact gttaaagaaa atggtaaaac tgttgatgtt aaagtcaaac aaaataagca
     7441 aacagactgg tcagaagata agttgaataa tataactttg aaaaagccga ctggtggtac
     7501 gattaaattg ggagatatcg ctacgttagt taaaacaacg acaccaagta aattgacgca
     7561 agaacaagga gattatgcaa cgacggtatc tgctaaagta acaaataaag atgtgggtgg
     7621 cacaacacga caagtgatgt ctaaaataaa taatttggac aaaccgaata atgtaaaggt
     7681 taatatcggt ggtgcatcag atgatattaa caatgcaatg acacaattag cctttgcaat
     7741 gttagctgca atcattatcg tatatttaat cctagttatt acatttaaag gtggcctagc
     7801 accatttaca attttattct ctttaccatt tacagttatc ggtgtaatta ttgcactatt
     7861 aatcacagga gaaacaatat cagtaccaag tttaattggt atgctaatgt taattggaat
     7921 cgtagtaaca aatgccattg tgttaataga ccgtgttatt aataatgagc aacagggcat
     7981 ggagatgaaa gaagcattaa tcgaagcagg cggtactaga attagaccga tattaatgac
     8041 ggcgattgca acaattggtg cattagttcc tttgttattt ggtcaagata gctcgattct
     8101 tatttcgaaa ggattagctg ctacagttat tggtggttta atttcatcaa ctttattaac
     8161 acttgtagtt gtaccagtaa tatatgaaat tctctttact ttaaaaaaac gattcactaa
     8221 acgatagata atataaaagc cataaaagcg gtcatgatat tgactacttt tatggctttt
     8281 taaaatttga aattttagac cttatttcac gtgcgattta atcgatgtgt taataaaaca
     8341 atactggcat tcaacatgta atccgataat aaaataaggt tgcaattatg ttattgcata
     8401 ttttgtttat tacttagaat aatacgatgg ttaagatgaa atatatgaca aagataaata
     8461 atgacattaa attcaagatg tttacgcctt ttagatcttt tttaagcatt ttaaatcctg
     8521 tcacttcgac attaccgact aagagtaata agacgataaa tacccaccaa ttctcttttg
     8581 gaaaactcat cgtcgttaat gctaagacga ataataacaa cacacccata ataattctca
     8641 aaatgcgagt aaatacaact tccattttaa aaatactaat aaaactaaca attagataaa
     8701 gtattgctag taaaatcaga taaaattgca tatgataact tcctctccgt ttcaaagggt
     8761 tcatgtagta cagcagcgta gaaaacataa atagcagtgt tgggatagat atgcgtgtat
     8821 gttgttaatg aaacactaaa tcataacacg acaacatctt gtgattctag atgtaattga
     8881 gcgaaaaatg aagcaattca tcaatataac gtattcagag caatgctagt caaatttatg
     8941 taacaaaaag catttattgc ttgaatgata gattattaat cttgcatttg agcacgaacg
     9001 tttctaattt cattttcaat atctttaagt tgacgaacaa gtgcggaagt atctaaattc
     9061 aaagattccc gtttttctat gtcaccttga agacgaacat aatcatattt aagttcggct
     9121 agttggtttt ctaaagacat actataacac tcctttgaaa tataattaag tgcatttaat
     9181 gtagattata gcatgaaaca gtagacttct aaaatatctg tggagtatgt taaagttata
     9241 aattatgaag attattgaaa gaagggataa cagtgacaga acaatttact atgattattt
     9301 tattaattgc gcttggttac tttttgaagc gcattaattt tataaaagca acagatagtc
     9361 aagtcattgc tacattagtt cttaatgtca cactcccatc tttagttatt gtgaatttaa
     9421 acagtgcaga attaaaattg tcattctcca ttttaccaat tttaatgatt atatatggta
     9481 ttgtggcgaa aatgattgct gtctggtttt ttagaaaata tgataatcac atgcgtggat
     9541 cagtaggtat gatgactggt gctatgaata ttggattgtt tgcatatcca ctaattgaag
     9601 tgatatggcc taaaacggga cttatttatt ttgttatggc tgatataggt ggtgcctttg
     9661 tgatgtttgg aattacttac tttgtaggta gctactttag tgaaggtagt gatcaattcg
     9721 attttaaata tttaggtaag aaattattac aatcagtgcc actcgttaca tacattgtaa
     9781 tgtttatatt gaatataagt cacatccatt taccacatgt ggcaatcgat ttctttagca
     9841 tactttccca tgcaaatatg ccgttatcaa tgattttgct aggtgtcatg ttgaatttta
     9901 ctctggaaag aaagtatata cctgctacta ttaaatattt atgtttgcat tatggattag
     9961 cattagttgc gggattgtta gtatatgtgt tgttaccagt atcagatgat atgattaaga
    10021 cgacattgtt ggtcacttgg atgttcccgg taggtgttgc aattatttct tatggtattc
    10081 agtttaaata tcgaacactg acatttatag gtatgacgac caatttgaca atcattatta
    10141 gtatcattat tttgtacgtg tatcaggcag tgtttgtcta gtttacacat ttgaaaataa
    10201 aatccatttt agtattcgta gaaaatgaga gcccccttta ccacaatatg taaatgtata
    10261 ttgtgaaaag ggggctttta tttatgacat agttgttcaa ctcatacatt caagttgata
    10321 atgcagtgcg ttatgcagct atccacttca tatgtatggc gcatacttga tatatgaata
    10381 tcatcaagat tttaaaagta tcgctaattt taaagtctta aaacgcatga ttaatgtcat
    10441 gatgtgttat tgcgttttgt ttttagagcc acgtatttcc taggacatat agaggaacat
    10501 tcaacgtaaa attcccgaaa attaatttta atcattctat aagtgaatgg ttattttcag
    10561 aagtgattac atttttacgt tacatgagta tttaactttc tatttcaaat tacctagaat
    10621 tatagcagct atcacgataa taataatacc tgcccaaata cccgtcatct gacgacgatc
    10681 ttttctttct cctaaaatga aaataccgcc taaggttgaa acgataacaa gtaactgtga
    10741 taatgagaaa cttgtagcta caccaacttt tggttgagaa tagaacatga acaagttacc
    10801 aattccccaa atcacacctg gcaatagatt aagtgctgtt gatttaagtg atgttttatg
    10861 attcatggat aggataaagc caccaattgc cataccgaca gattggaaga acaatgcatc
    10921 agttccacca acaccaaaga tgtcaccaag tacaacgaaa cctacatatc caactgtaga
    10981 tacaattaaa atacccattg cttttttaaa ttcaggatta tctgattgac gttcattttt
    11041 agctttaagt gaagtaagtg ctacaccagt aactaataat atcatggcga ttaaaccaaa
    11101 gataatttga gtcattgaag accattcacc taaaaagata acgctgaata atgttgtacc
    11161 aactaattgc atacctgtag aaactggcat agtatttgat acaccgatta aactaataga
    11221 tttaagctga ttcgcttgtc caaacgccca taatgcacca gaaataagac cgacaataat
    11281 tacagtagga ttattgaatt ttgcgaaacc agtaataagt aaacctaatc caacaatgag
    11341 tgcgcctagc gtcgtaccac gaatttggtt gtaaggtcca ccgccgacga acacattaat
    11401 aagaacgaca cttccccaga ataaagcagg taaaagtgcg attaagaaat caagaaattg
    11461 caagactact cactctccta tactatttat gcaagtgctc cccttaattg acacttgcat
    11521 gttattgtaa agcattctgc cctaatagta aaaaacaact acttataatt ttcaatattt
    11581 acaaaaagta gaaaaaccca caatacatgc taatgatgta ctgtagggta tttgcttggt
    11641 gttttattca gggattgttt gatcttttac gttacgtgat tcgaatttga atttaatgta
    11701 ctcatagttg atatggaaac ctagaccatt aaatcgttcg atgagtttat tatttttatt
    11761 acgaatttta aaataaactt ttctaatatc aaaattatca aaagcatatt gcaatgcata
    11821 cgcaagcaac tcgaaagcga tacctttcaa acggtaatct acatgagaac tgaagtattt
    11881 aatctcggcg attgactgtt gtgaatcaat ttcaaggtat aaataacctt taagtaaacc
    11941 ttcgctaaca aataaaaaca aatgatgatg gtcgtctaaa ctatcaatga tatcttgtgg
    12001 tgactgtgcc tgatatttaa atgtagttgt gtgtaatttg ctgaaagcac gataaaaccc
    12061 tttgtgataa ggaatgatat ttggttggtg catatcttct tctaatcttg tacgggcttc
    12121 tatgtaatag tcagtgaagt tataacttgc ttgaataact ttcattaatg gtttgtattg
    12181 ttgaatgcct tcttcaaaag aaaagttaaa gacggcatcg tcaggttggt tcgaagtcat
    12241 tgctttaaat agcgtttcaa acgtatcgac agataataca taattgtcag ccacgaaagg
    12301 gcctatgact ttgaatttat ttttatcgta tgcaaaagac aatatgattg ctttgattga
    12361 atcgttttct ttaatcgaaa atacaccagg tgattcaatc gcatcggtta ttaatacatc
    12421 tatttcattg tactgttgag gaagtttata taagtatgat gctgattcat aattcgaatg
    12481 ttcaatgaac gattttattt gtgtaatatc atataactga actatgctca tgtcattacc
    12541 tccgtacttt ttgttacttt tattatatag tatttcaact gaaatgaaag ttaatagtga
    12601 tattaacatg ttacaataca tttaacacat catttgcaat cattgaattt aaatcaaaga
    12661 ttagtggaat agatgaagca cgttcgaaat aaaagaacgt atgagaaagg ataatttatg
    12721 aaatctttaa tattagctga aaaaccatca gtcgcaagag atattgctga tgctttacaa
    12781 ataaatcaga agcgtaatgg ttactttgaa aataaccaat atattgtcac gtgggcgtta
    12841 ggtcatctag tgacaaatgc gacacctgaa caatacgata aaaatttaaa ggaatggcga
    12901 ttagaagacc ttccaattat acctaaatat atgaaaactg ttgttattgg taaaacaagc
    12961 aaacaattta aaacagtaaa agcgttaatt ttagataata aagtgaaaga tattattatt
    13021 gcaacagatg ctggacgaga aggtgaacta gttgcaagat tgattttgga taaagttggt
    13081 aacaaaaagc caatccgtcg attatggatt agctcagtta ctaaaaaagc tattcaacaa
    13141 ggttttaaaa atttaaaaga cggtcgtcaa tataacgatt tgtattatgc agcgttagcg
    13201 agaagtgagg cagattggat tgttgggatt aatgcaacgc gtgcactaac aacaaagtat
    13261 gatgcacagc tatccctggg acgtgttcag acaccaacga ttcaattagt aaatacacga
    13321 caacaagaga ttaatcagtt caaaccacaa caatacttta cattatcatt aacggtaaaa
    13381 gggtttgatt ttcagctaga atcaaatcag cgatatacca ataaagaaac tttagaacag
    13441 atggttaata atttgaaaaa tgtcgatggt aagattaaat ctgttgctac taaacataag
    13501 aagtcgtatc cgcaatcact gtacaattta acagatttac aacaagatat gtatagacgt
    13561 tataaaattg gacctaaaga aacattgaat acacttcaaa gcttatatga gagacataaa
    13621 gtcgtaacct atccaagaac agattcaaac tatttaacaa ctgatatggt agatactatg
    13681 aaagaacgta ttcaggcgac gatggcaaca acatataaag accaagcacg cccattaatg
    13741 tctaaaacat tttcatcaaa aatgtcgata tttaataatc aaaaagtatc tgatcaccat
    13801 gcaattattc ctacagaagt gagacctgtc atgtcagact taagtaatag agaattaaag
    13861 ttatacgata tgattgtcga gcgtttttta gaagctttaa tgcctccgca cgagtatgac
    13921 gcgataactg taactttaga ggttgcaggg cacacatttg ttttgaaaga gaatgtaaca
    13981 actgttttag gttttaaatc tattagacaa ggtgaatcta ttacagagat gcaacagcct
    14041 ttttcagaag gcgatgaagt gaagatttca aaaacaaata ttagagaaca tgaaacaaca
    14101 cctccagaat attttaatga aggttcgtta ttaaaagcga tggagaaccc tcagaacttt
    14161 attcaattga aggataaaaa atatgcgcaa actttaaaac aaacaggtgg tatcggcaca
    14221 gttgcaacaa gggccgacat tatcgataaa ttatttaata tgaatgccat tgaatcaaga
    14281 gacggtaaaa ttaaagtaac gtcaaaaggt aaacaaatat tagaattagc accagaagaa
    14341 ttaacgtcgc cacttttaac tgcacaatgg gaagaaaaat tacttttaat tgaacgtggt
    14401 aaatatcagg cgaaaacatt tattaatgaa atgaaagatt ttacgaaaga tgttgtaaat
    14461 gggattaaaa atagtgatcg taaatataaa cacgataatt taacaaccac agaatgccca
    14521 acgtgtggta aattcatgat taaagttaaa actaaaaatg gtcagatgct tgtgtgccaa
    14581 gatccatctt gtaagacgaa aaagaatgta cagcgcaaaa caaatgcaag atgtccaaac
    14641 tgtaaaaaga aattaacgtt gtttggtaaa gggaaagaag cggtatatcg ttgtgtttgt
    14701 ggacattctg aaacgcaagc acatatggat cagcgtatga agtctaaatc ctctggtaaa
    14761 gtatctcgta aagaaatgaa aaagtatatg aataaaaatg aaggtttaga caataatccg
    14821 tttaaagatg cattaaagaa cttgaattta tagataaaat cgaacaaagt tgaatcagaa
    14881 aaacgaaaag ttcgcttttg gtattgtttt ttattaagaa tgatattaaa ctattaaggt
    14941 attttaaaaa aaggagcatc cattcgtgaa aaactatttc cagttcgata aatatggaac
    15001 aaactttaaa agagaaatct taggcggtat cacaactttc ttatctatgg cctatatttt
    15061 agcagttaac ccgcaagttt taagtttagc aggtgttaaa ggcgtatcag aagatatgaa
    15121 aatggaccaa ggtgccattt ttgtagcgac tgcattagca gcatttgtag gctcgctatt
    15181 catggggcta atagctaaat atccaattgc gttagcacca ggtatgggat taaatgcatt
    15241 ctttgcattt actgttgtgt taaccatggg tattccttgg caagttggtt taacaggcgt
    15301 tttattctca ggtatattct ttgcgatatt aaccgtcaca gggtttaggg aagttattat
    15361 taatgcaatt ccttatcaaa tgaagatggc tgtttcagca ggtataggat tattcattac
    15421 atttgtaggt ttacaaagtt cgggtattat agttaaaaat gaatctacat tagtaacatt
    15481 gggacattta actgatccac cagtactttt ggcgatattc ggtatcgtta ttacagttat
    15541 tttatatgca attaaattgc cgggttctat ctttatcggt atgatcatca cagcaatcgt
    15601 tggtatgttc actggattga ttcaaatgcc atctggtatt gtgggtaaga ttccaagtat
    15661 tgaaccaacg ttcggtgctg catttgaagc gtttaaagac ccaagtcaat tattaacgat
    15721 acagttctta attgttattt taacgttctt atttattgat ttctttgata cagctggtac
    15781 attagtagct gttgctactc aagcaggtat tatgaaagat aataaattac caagagctgg
    15841 tagagcattg ttctcagatt ctttagctac gattgtagga tcagtttttg gaacaactac
    15901 aacaacttct tatatcgaat caacttcagg tgttgctgta ggtgctagaa caggatttgc
    15961 tagtatcgta acaggttgtt gtttcctatt agcgctgttc tttagtccgt taatcgctgt
    16021 tgtaacaagt gcagttacga caccagcctt agtggtggta ggtgtattaa tggctgcaaa
    16081 ctttgctgag ataaactgga aaagttttga agttgcagtg ccagccttca ttacaatcat
    16141 tatgatgcct ttatcatatt caattgcaac tggtattgcg tgtggattta ttttctatcc
    16201 aattacaatg cttatctcta aaaaacataa agaagtacat ccgataatgt atgtattaat
    16261 ggtactattc attctgtact tcatatttgt tcatggttaa atccagacac aaaaaacgac
    16321 tgccaatgat tggtagtcgt ttttttcttt gaagtaagga taaaataaga agcgaaataa
    16381 cataaagcga tattatgaat gtctttaaat atccgatata aatatacttc ttttaaaatt
    16441 gttggacgtg tttcttctat attatatacc taattaaaaa ggttttaaga attattctct
    16501 atgtccatct ttgtattgtg tgtaaatgac acgagcattt ttattaggta tataggttgg
    16561 cgctgataat acaactagga caaatagaat cattaggaaa aataaaatcc aatgaatcaa
    16621 aattcctaag aaggggataa tagctaaaaa actacctact ataccaaata ttggaataat
    16681 cgctataggt ctaatcgcat tttgtctatt cactaataat ataatagtaa gaattagata
    16741 aagtaatgca ttaaagagta aaggctgcca tccaaaactt aaaataaacg ttgcacctaa
    16801 aaaaggaatg ccatagaaaa actcacaaag tattaaaaag atacctataa gtgctaaaga
    16861 taattttggt ccatttttca tattcattca ccaccgttct tatgactaat tatatagaat
    16921 aattttcaat gataataata aaatgattca aggtacgaat ttacaataaa gaaggaaata
    16981 aaaaatatct cgaaaatagt tgaactgact aagtatataa agtaaaatat aaaagtatgt
    17041 gttagacaga tagaaaaatt atgtgaaaag ccttgattta tcgatgtttt tcgtgtaata
    17101 taactaacgt tggactttaa gaaaagcgat gaagcgaaag gttactgaca cacccggccg
    17161 ctttgccatg gcgctgtgta agatagtttt cgtggagaag tctatcacta aatgtagacg
    17221 aataaggagg gaaaattatg gcaaaacaaa aaatcagaat cagattaaaa gcttatgatc
    17281 accgcgtaat tgatcaatca gcagagaaga ttgtagaaac agcgaaacgt tctggtgcag
    17341 atgtttctgg accaattccg ttaccaactg agaaatcagt ttacacaatc atccgtgccg
    17401 tgcataagta taaagattca cgtgaacaat tcgaacaacg tacacacaaa cgtttaatcg
    17461 atattgtaaa cccaacacca aaaacagttg acgctttaat gggcttaaac ttaccatctg
    17521 gtgtagacat cgaaatcaaa ttataataga caattttagg aggtggactt tcgatgacca
    17581 aaggaatctt aggaagaaaa attgggatga cacaagtatt cggagaaaac ggtgaattaa
    17641 tccctgtaac agtagtagaa gctaaagaaa atgttgtatt acaaaagaaa actgtagaag
    17701 ttgatggata caacgcaatc caagttggat ttgaagacaa aaaagcatac aaaaaagatg
    17761 caaaatctaa taaatatgct aataaaccag ctgaaggtca cgctaaaaaa gctgacgcag
    17821 cacctaagcg cttcattcgt gaattccgca atgtagacgt ggatgcttac gaagtaggtc
    17881 aagaagtctc agtagatact tttgtagctg gcgacgttat tgacgtaaca ggcgtatcaa
    17941 aaggtaaagg tttccaaggt gcaattaaac gccacggaca atctcgtgga cctatgtcac
    18001 acggttctca tttccacaga gcaccaggtt ctgtaggtat ggcttcagat gcttctagag
    18061 tatttaaagg ccaaaaaatg ccaggacgta tgggtggaaa cactgtaact gttcaaaact
    18121 tagaagtagt tcaagttgac acagaaaaca aagttatctt agtaaaaggt aacgtacctg
    18181 gacctaaaaa aggtttagta gaaatcagaa cttcaattaa aaaaggtaat aaataataag
    18241 aagtgaaagg aggaaatgca taatggctaa ttatgatgtt ttaaaattag acggaactaa
    18301 atcaggttca atcgaattaa gcgatgcagt attcggtatt gagccaaata atagcgtttt
    18361 attcgaagct attaatttac aacgtgcttc attacgtcaa ggtacgcatg ctgttaagaa
    18421 tcgttcagca gtaagcggtg gcggacgtaa accatggaag caaaaaggaa caggtcgtgc
    18481 tcgtcaaggt acaatccgtg ctccacaatg gcgtggcggt ggtatcgtat tcggaccaac
    18541 tccaagaagt tatgcataca aaatgcctaa gaaaatgcgt cgtttagctt tacgctcagc
    18601 attatctttc aaagctcaag agaatggctt aactgtagtt gacgcattca acttcgaagc
    18661 tccaaaaact aaagaattca aaaatgtatt atctacatta gaacaaccta aaaaagtatt
    18721 agtagttact gaaaacgaag atgtaaatgt tgaattatca gcacgcaaca tccctggcgt
    18781 tcaagtgaca actgctcaag gtttaaatgt tttagatatc actaatgctg acagcttagt
    18841 aattactgaa gctgctgcta aaaaagttga ggaggtgctc ggataatgga agcaagagat
    18901 attcttaagc gccccgtaat cactgagaaa tcttctgaag caatggctga agacaaatac
    18961 actttcgacg ttgatactcg tgttaacaaa acacaagtaa aaatggcagt tgaagaaatc
    19021 ttcaacgtaa aagttgcaag tgttaatatc atgaattaca aacctaagaa aaaacgtatg
    19081 ggccgttacc aaggctatac aaacaaaaga agaaaagcga ttgtaactct taaagaagga
    19141 tcaatcgact tatttaacta ataaaacaat taccattaag gaggtaagcg acaatggcta
    19201 ttaaaaagta taagccaata acaaatggtc gtcgtaatat gacttcgtta gatttcgcag
    19261 aaatcacgaa aactacacct gaaaagtcat tattaaaacc gctaccgaaa aaagcgggac
    19321 gtaacaacca aggtaaattg actgtaagac accatggtgg tggacacaaa cgtcaatacc
    19381 gtgttatcga tttcaaacgt aacaaagatg gtatcaatgc aaaagttgat tctattcaat
    19441 atgatccaaa ccgctcagca aacatcgctt tagttgtata tgcagacggt gaaaaacgat
    19501 atatcattgc tcctaaagga ttagaagtag gtcaaatcgt tgaaagtggt gctgaagctg
    19561 acatcaaagt tggtaacgca ttaccattac aaaacattcc agttggtaca gtagtacaca
    19621 acatcgagct taaacctggt aaaggtggac aaatcgctcg ttcagctggt gcaagtgctc
    19681 aagtacttgg taaagaaggt aaatacgtat taatcagatt aagatctggt gaagttcgta
    19741 tgatcttatc tacttgccgt gctacaatcg gtcaagttgg taacctacaa cacgaattag
    19801 ttaacgttgg taaagccgga cgttcaagat ggaaaggtat ccgtccaaca gttcgtggtt
    19861 ctgtaatgaa ccctaacgat cacccacacg gtggtggtga aggtcgtgct cctatcggta
    19921 gaccatctcc aatgtcacca tggggtaaac ctacgcttgg taagaaaact cgtcgtggta
    19981 aaaaatcatc agacaaactt atcgttcgtg gacgtaagaa aaaataatat aatcaactta
    20041 tttgggtgtg cggcttaaag ctgcacgcac ataataagaa gggaggcgcc caaatggctc
    20101 gtagtattaa aaaaggacct ttcgtcgatg agcatttaat gaaaaaagtt gaagctcaag
    20161 aaggaagcga aaagaaacaa gtaatcaaaa catggtcacg tcgttctaca attttcccta
    20221 atttcatcgg acatactttt gcagtatacg acggacgtaa acacgtacct gtatatgtaa
    20281 ctgaagatat ggtaggtcat aaattaggtg agtttgctcc tactcgtaca ttcaaaggac
    20341 acgttgcaga cgacaagaaa acaagaagat aatatctatt aagtagagga ggacatccta
    20401 atggaagcaa aagcggttgc tagaacaata agaatcgcac ctcgtaaagt aagactagtt
    20461 cttgacttaa tcagaggtaa aaatgctgct gaagctattg caattttaaa attaacaaac
    20521 aaagcttcat caccagtaat tgaaaaagta ttaatgtccg ctttagctaa tgctgaacat
    20581 aactatgaca tgaacacaga tgaattagta gttaaagaag catatgctaa cgaaggacca
    20641 acattaaaac gtttccgtcc acgtgcgcaa ggtcgtgcaa gtgcgattaa caaacgtaca
    20701 agccacatta caatcgtcgt aagtgacggt aaagaagaag ctaaagaagc ttaattaact
    20761 tttaaggagg gaatactgtg ggtcaaaaaa ttaatccaat cggacttcgt gttggtatta
    20821 tccgtgattg ggaagctaaa tggtatgctg aaaaagactt cgcttcactt ttacacgaag
    20881 atttaaaaat ccgtaaattt attgataatg aattaaaaga agcatcagtt tctcacgtag
    20941 agattgaacg tgctgcaaac cgtatcaaca ttgcaattca tactggtaaa cctggtatgg
    21001 taattggtaa aggcggttca gaaatcgaaa aattacgcaa caaattaaat gcgttaactg
    21061 ataaaaaagt acacatcaac gtaattgaaa tcaaaaaagt tgatcttgac gctcgtttag
    21121 tagctgaaaa catcgcacgt caattagaaa accgtgcttc attccgtcgt gtacaaaaac
    21181 aagcaatcac tagagctatg aaacttggtg ctaaaggtat caaaactcaa gtatctggtc
    21241 gtttaggcgg agctgacatc gctcgtgctg aacaatattc agaaggaact gttccacttc
    21301 atacgttacg tgctgacatc gattatgcac acgctgaagc tgacactact tacggtaaat
    21361 taggcgttaa agtatggatt tatcgtggag aagttcttcc tactaagaac actagtggag
    21421 gaggaaaata ataatgttac taccaaaacg tgtaaaatat cgtcgtcaac atcgtcctaa
    21481 aacaactggt cgttctaaag gcggtaacta cgtaacattt ggtgagtttg gtttacaagc
    21541 tacaacaacg tcttggatca catctcgtca aatcgaatct gctcgtatag caatgacacg
    21601 ttacatgaaa cgtggcggga aagtttggat taaaatcttc ccacatacac catatactaa
    21661 aaaaccttta gaagtacgta tgggtgctgg taaaggtgcg gttgaaggct ggatcgcagt
    21721 tgttaaacca ggtagaattt tattcgaagt tgctggcgtt tctgaagaag ttgcgcgtga
    21781 agcactacgt ttagcaagtc acaaacttcc agtaaaaact aagtttgtaa aacgtgagga
    21841 attgggtggt gaaacaaatg aaagctaagg aaattagaga cttaaccact tcagaaatcg
    21901 aagaacaaat caaatcttca aaagaagagc tttttaacct acgctttcag ttagctacag
    21961 gtcaattaga agaaactgca cgtattcgta cagtaagaaa aacgattgca cgtctaaaaa
    22021 ctgttgctcg tgaaagagaa attgaacaaa gtaaggctaa tcaataatta agtaagagga
    22081 ggttacaaaa gtgagcgaaa gaaacgatcg taaagtttat gtaggtaaag ttgtttcaga
    22141 caaaatggac aagactatta cagtacttgt tgaaacttac aaaacacaca aattatacgg
    22201 taaacgagta aaatactcta aaaaatacaa aactcatgat gaaaacaatt cagctaaatt
    22261 aggagacatt gttaaaattc aagaaactcg tcctttatca gcaacaaaac gttttcgttt
    22321 agtagagatt gttgaagagt cagtaattat ttaatacaag tttagagata agggaggttt
    22381 aactaatgat ccaacaagaa acacgcttga aagtagcaga caactctggt gctcgtgaag
    22441 ttcttacaat caaagtatta ggtggatctg gtcgtaaaac agcaaacatc ggcgatgtta
    22501 tcgtatgtac tgttaaaaat gcaacaccag gtggcgttgt taaaaaaggt gacgttgtca
    22561 aagctgtaat cgtacgtact aagtcaggtg ttcgtcgtaa tgacggttca tacatcaaat
    22621 ttgatgaaaa tgcatgtgtt atcatccgtg atgacaaagg cccacgtggt actcgtatct
    22681 tcggacctgt tgctcgtgaa ttacgtgaag gtaacttcat gaaaatcgta tcattagcac
    22741 cagaagtact ttaattaata acaaccaata caaataagga ggtgcccaca tgcatatcaa
    22801 aaaaggtgac aacgttaaag ttatcgcagg taaagacaaa ggtaaagaag gtaaagtaat
    22861 tgctactcta cctaaaaaag accgtgtcgt tgtggaaggt gttaacatta tgaaaaaaca
    22921 ccaaaaacca actcaattaa atcctgaagg tggaatctta gaaacagagg cagcaatcca
    22981 tgtttctaat gtacaattat tggaccctaa aacaaacgaa ccaactcgtg taggttacaa
    23041 atttgttgat ggtaaaaaag ttcgtatcgc taaaaaatct ggcgaagaaa ttaaatctaa
    23101 taattaataa caagttgaaa ggaggatccc actttgaacc gtttaaaaga aaagtttaac
    23161 actgaagtta ctgaaaactt aatgaaaaaa ttcaattata gttcagtaat ggaagtacca
    23221 aaaatagata aaatcgttgt gaacatgggt gtaggtgacg cagtacaaaa ttctaaagta
    23281 ttagacaatg ctgttgaaga attagaattg atcactggtc aaaaaccatt agtaactaaa
    23341 gctaaaaaat caatcgcgac tttccgttta cgtgaaggta tgccaatcgg tgcgaaagta
    23401 acacttcgcg gtgaaagaat gtatgaattc ttagacaaat taatttcagt atcattacca
    23461 cgtgtacgtg acttccaagg tgtttctaaa aaagcatttg acggacgcgg taactacact
    23521 ttaggtgtta aagaacaatt aattttccca gaaatcgact atgataaagt aagtaaagtt
    23581 agaggaatgg atattgttat cgtaacgact gctaacactg atgaagaagc tcgtgaattg
    23641 ttagctaact tcggtatgcc attccgtaaa taattattta aaggaggcta attaagtggc
    23701 taaaacttca atggttgcta agcaacaaaa aaaacaaaaa tatgcagttc gtgaatacac
    23761 tcgttgtgaa cgttgtggcc gtccacattc tgtatatcgt aaatttaaat tatgccgtat
    23821 ttgtttccgt gaattagctt acaaaggcca aatccctggc gttcgtaaag ctagctggta
    23881 ataaaaaaga gtctgaaagg aggcaacaat caatgacaat gacagatcca atcgcagata
    23941 tgcttactcg tgtaagaaac gcaaacatgg tgcgtcacga gaagttagaa ttacctgcat
    24001 caaatattaa aaaagaaatt gctgaaatct taaagagtga aggtttcatt aaaaatgttg
    24061 aatacgtaga agatgataaa caaggtgtac ttcgtttatt cttaaaatat ggtcaaaacg
    24121 atgagcgtgt tatcacagga ttaaaacgta tttcaaaacc aggtttacgt gtttatgcaa
    24181 aagctagcga aatgcctaaa gtattaaatg gtttaggtat tgcattagta tcaacttctg
    24241 aaggtgtaat cactgacaaa gaagcaagaa aacgtaatgt tggtggagaa attatcgcat
    24301 acgtttggta ataaaaaata aggaggtgcc ataacatgag tcgtgttggt aagaaaatta
    24361 ttgacatccc tagtgacgta acagtaactt ttgatggaaa tcatgtaact gttaaaggtc
    24421 ctaaaggtga attatcaaga actttaaatg aaagaatgac attcaaacaa gaagaaaaca
    24481 caattgaagt tgtaagacca tctgattcta aagaagatag aacaaaccat ggtacaactc
    24541 gtgctttatt aaacaatatg gtacaaggtg tttctcaagg atacgtaaaa gtacttgaac
    24601 ttgttggtgt aggttaccgt gctcaaatgc aaggtaaaga cttaatcctt aacgttggtt
    24661 attctcaccc agtagaaatt aaagctgaag aaaacattac tttctcagtt gagaaaaaca
    24721 cagtcgttaa agttgaaggt atttcaaaag aacaagttgg agcattagca tctaacatcc
    24781 gttcagtaag acctccagag ccttacaaag gtaaaggtat tcgttaccaa ggtgaatacg
    24841 ttcgccgtaa agaaggtaaa actggtaaat aatagataaa ctctaaagaa aggagtattg
    24901 aaatgatcag taaaattgat aaaaataaag tgcgtttaaa aagacatgct cgtgttcgta
    24961 ctaacttatc aggtacagct gaaaagccac gtttaaacgt atatcgttca aacaagcata
    25021 tctacgctca aattattgat gataataaag gcgtaacatt agctcaagct tcttcaaaag
    25081 acagcgacat tgctactaca gcaactaaag ttgaattagc aactaaagtc ggtgaagcaa
    25141 ttgctaaaaa agctgctgac aaaggcatta aagaaatcgt atttgaccgt ggaggatatt
    25201 tatatcacgg acgtgttaaa gcattagctg aagcagcaag agaaagcgga ttagaatttt
    25261 aatttaaagg agggacaaat acatggctcg tagagaagaa gagacgaaag aatttgaaga
    25321 acgcgttgtt acaatcaacc gtgtagcaaa agttgtaaaa ggtggtcgtc gtttccgttt
    25381 cactgcatta gttgtagttg gagacaaaaa tggtcgtgta ggtttcggta ctggtaaagc
    25441 tcaagaggta ccagaagcaa tcaaaaaagc tgttgaagca gctaaaaaag atttagtagt
    25501 tgttccacgt gttgaaggta caactccaca cacaattact ggccgttacg gttcaggaag
    25561 cgtatttatg aaaccggctg cacctggtac aggagttatc gctggtggtc ctgttcgtgc
    25621 cgtacttgaa ttagcaggta tcactgatat cttaagtaaa tcattaggat caaacacacc
    25681 aatcaacatg gttcgtgcta caatcgatgg tttacaaaac cttaaaaatg ctgaagatgt
    25741 tgcgaaatta cgtggcaaaa cagtagaaga attatacaat taaggaggga aaactagtta
    25801 tggctaaatt acaaattacc ctcactcgta gtgttattgg tcgtcctgaa acacaacgta
    25861 aaactgttga agctttaggt cttaaaaaga ctaacagttc agtagttgtt gaagataacc
    25921 ctgctattcg tgggcaaatc aacaaagtta agcacttagt aacagtagaa gaaaaataat
    25981 caaggaggtg ccgaaatgaa attacatgag ttaaaaccgg cagaaggttc acgtaaagaa
    26041 cgcaatcgtg ttggacgtgg tgttgcgaca ggtaacggta aaacaagtgg tcgcggacac
    26101 aaaggtcaaa aagctcgttc aggcggtggt gtaagaccag gatttgaagg tggtcaatta
    26161 ccattattcc gtcgtttacc aaaacgtggt tttactaaca taaatcgtaa agaatatgct
    26221 attgttaact tagaccaact taataaattt gaagatggta ctgaagtaac tccagcttta
    26281 ttagtagaat ctggtgttgt taagaatgaa aaatctggta tcaaaatact aggtaatggt
    26341 tcacttgata agaaattgac agtgaaagct cataaattct cagcttcagc agcagaagct
    26401 attgatgcta aaggtggagc acacgaggtg atctaatgat tcaaaccctt gtgaacttct
    26461 ttagaacaaa agaggttcgt aacaagattt tcttcacact agcaatgtta gtaattttta
    26521 aaatagggac ttatatacca gctccaggag taaatcctgc agcttttgat aatccccaag
    26581 gttctcaagg tgccactgag ttattaaata cttttggtgg cggagccttg aaacgatttt
    26641 ctatttttgc aatgggtatt gtaccctaca tcactgcatc aatcgtaatg caattattac
    26701 aaatggatat tgtccctaaa ttctcagaat gggcaaaaca aggtgaagta ggtagaagaa
    26761 agttaaataa cgttactcgt tatttagcaa tttctttagc atttatccaa tctataggta
    26821 tggcattcca atttaataat tatctcaaag gtgcgctgat tatcaatcag tcaattatga
    26881 gttatttatt aatagcacta gttttgacag caggaactgc tttcttaata tggcttggtg
    26941 atcaaatcac tcagttcggt gttggtaatg gtatttctat tatcatattt gcgggtattt
    27001 tatcaacgtt gccagccagt ctaattcaat ttggacaaac tgcgttcgtt gggcaagaag
    27061 atacatcatt agcatggttg aaagtattag gacttttagt gtcactgata ctattaactg
    27121 taggtgctat ttatgtactt gaagccgttc gtaaaattcc gattcaatat gctaagaaac
    27181 aaactgctca aagattaggt tcacaggcaa cttatctacc tttaaaagtg aactcagccg
    27241 gtgttattcc tgtaatcttt gcaatggcgt tcttcttact gcctagaaca ttaacgttgt
    27301 tctatccaga taaagaatgg gcacaaaaca ttgcgaatgc tgcaaatcct tcaagtaatg
    27361 ttggtatggt tgtatatatt gtactaatta tactattcac atacttctat gcatttgtac
    27421 aagttaatcc ggaaaaaatg gctgataacc ttaagaaaca aggtagctat gttccaggta
    27481 ttagacctgg tgaacaaacc aaaaaatata ttactaaagt tttatatcgt ttaacttttg
    27541 taggttctat cttcttagcc gttatttcaa ttctaccgat attagcaact aagtttatgg
    27601 gattaccaca atcaattcag attggtggta caagtttact tatcgttatt ggtgtagcga
    27661 ttgaaacaat gaaatcttta gaagctcaag tatctcaaaa agaatataaa ggctttggtg
    27721 gtagataatt ttaggagggc acttatgaat atcattttga tgggtttacc tggcgcaggt
    27781 aaaggaactc aagcaagtga aattgtcaag aaattcccaa taccccacat ttcaactggt
    27841 gacatgttca gaaaagctat aaaagaagaa actgaattag gtaaagaagc taagtcttat
    27901 atggaccgtg gcgaattagt tcctgatgaa gtgactgtag gtatcgttaa ggaaagaatt
    27961 tctgaagacg atgcaaaaaa aggcttttta ttagatggct tcccaagaac aatcgagcaa
    28021 gctgaggcat taaataatat tatgtctgag cttgacagaa acattgatgc tgtcatcaat
    28081 atcgaagttc cggaagaaga attaatgaac cgtcttacag gtcgtcgaat ctgtgagtca
    28141 tgtggtacaa cgtatcatct tgtatttaat cctccgaagg tcgaaggtat ttgtgatatc
    28201 gatggtggta aattgtatca acgagaagat gataatcctg aaacggtagc taatcgtttg
    28261 agtgttaata ttaaacaatc taagcctatt ttagatttct atgatcaaaa aggtgtattg
    28321 aaaaatattg atggttcaaa agatattagc gacgttacca aagatgtcat tgatatttta
    28381 gatcatttgt aatagatcaa cttttagcgg tctattttaa gtgaggcatg atttaatcat
    28441 attagataga tgagagtggc aattaacgat aattaattcc cgtaattttt gtttaactta
    28501 taaaattaat cactgataga ttcgtaacaa taaagttgtc tattgacgat taccttacta
    28561 ttgcaaaaag ggggaagtta atcaatggct aaacaagatg taattgaatt agaaggtact
    28621 gtattagata ctttaccgaa cgcaatgttt aaagtagaat tagaaaatgg tcatgagatt
    28681 ttagctcacg taagtggtaa aatcagaatg aattacattc gtattctacc tggcgacaaa
    28741 gtaactgttg agatgtctcc gtacgattta acacgcggaa gaattactta tcgttataaa
    28801 taatcgtcac tccataatat agggaggtat aaaaatgaaa gtaagaccat cagtaaaacc
    28861 tatttgcgaa aaatgtaaag tcattaaacg taaaggtaaa gtaatggtaa tttgtgaaaa
    28921 tccaaaacac aaacaaagac aaggttaata aaagagaggt gtaaattaat atggcacgta
    28981 ttgcaggagt agatattcca cgtgaaaaac gcgtagttat ctcattaact tatatatacg
    29041 gtatcggtac gtcaactgct caaaaaattc ttgaagaagc taacgtatca gctgatactc
    29101 gtgtgaaaga tttaactgat gacgaattag gtcgcatccg tgaagttgta gacggttata
    29161 aagtcgaagg tgacttacgt cgtgaaacta acttaaatat caaacgttta atggaaattt
    29221 catcataccg tggtatccgt caccgtcgtg gtttaccagt tcgtggtcaa aaaacgaaaa
    29281 acaacgcgcg tactcgtaaa ggaccagtta aaacggtagc taacaagaaa aaataatagg
    29341 taaaggaggc aaattttaaa tggcacgtaa acaagtatct cgtaaacgta gagtgaaaaa
    29401 gaatattgaa aatggtgtag cacacatccg ttcaacattc aacaacacta ttgtaactat
    29461 cactgatgag ttcggtaatg ctttatcatg gtcatcagct ggtgcattag gattcaaagg
    29521 atctaaaaaa tcaacaccat ttgcagcaca aatggcttct gaaactgcat ctaaatcagc
    29581 tatggagcat ggtttaaaaa cagttgaagt aacagttaaa ggacctggtc caggtcgtga
    29641 atcagctatt cgtgcattac aatctgcagg tttagaagta actgcgatca gagacgttac
    29701 tccagtacct cataacggtt gtcgtccacc aaaacgtcgt cgtgtataat ttatgatggt
    29761 attgttacag gtcactgagc aaacatttta aattaagtcg acgtatataa ggaggatatt
    29821 taaatgatag aaatcgaaaa acctagaatt gagacaattg aaattagtga agatgctaaa
    29881 ttcggtaagt tcgttgttga accactagaa cgtggctacg gtactacact aggaaactcc
    29941 ttacgtcgta tcctactatc ttcattacca ggtgcagccg ttaagtatat tgaaattgag
    30001 ggagttttac atgaattctc agcagtagac aatgtagttg aagatgtttc tacaattatt
    30061 atgaacatta aacaattagc attgaaaatt tactctgaag aagataaaac tttagaaatt
    30121 gatgtacgtg atgaaggcga agtaacagca agcgacatta cacatgatag tgatgttgaa
    30181 attttaaacc cagagcttaa aattgcaaca gtatctaaag gtggtcactt aaaaattcgt
    30241 ctagttgcta acaagggtag aggttacgca ttagcagaac aaaataatac tagtgattta
    30301 ccaattggtg taatccctgt tgattcattg tattcacctg ttgaacgtgt gaactatact
    30361 gttgaaaata cacgtgtagg tcaaagcagt gattttgata aattaacatt ggatgtttgg
    30421 actaatggtt caatcacacc acaagaatca gtttcattag cagcaaaaat aatgactgaa
    30481 cacttgaata tcttcgttgg tcttactgat gaagcgcaaa acgctgaaat catgattgaa
    30541 aaagaagaag atcaaaaaga aaaagtatta gaaatgtcta ttgaagaatt agacttatct
    30601 gtacgttcat ataactgctt aaaacgcgca ggaatcaatt ctgttcaaga gttagctgac
    30661 aaatctgaag ctgacatgat gaaagtgcgt aatttaggtc gtaaatcttt agaagaagtt
    30721 aaatacaaat tagaagattt aggattagga ttaagaaaag aagattgata aaggaggtta
    30781 actcatgggt tacagaaaat taggtcgtac ttctgatcaa cgtaaagcta tgttacgtga
    30841 cttagctaca tcacttatta ttagtgaacg tattgaaact acagaagctc gtgcaaaaga
    30901 agttcgcagt gttgttgaga aattaatcac tttaggtaaa aaaggagatt tagcttctcg
    30961 tcgtaatgca gctaaaactt tacgtaatgt tgaaatctta aacgaagatg aaactacaca
    31021 aactgcactt caaaaattat ttggtgaaat cgcagagcgt tacacagaac gtcaaggtgg
    31081 ttacactcgt atccttaaac aaggccctcg tcgcggtgac ggtgctgaat cagtaattat
    31141 cgaattagta taagaattta tataaataca ctaactactt ttatataaag caaatgagtg
    31201 caacgataat gtttgccaca tacaaatatt gtctagctca gagtacccca tcaactaaat
    31261 aaagatttaa agcgtgaact caacaaactt atgatggggt gcgcgctttt ttattgaata
    31321 aaatatcgga cttatgaaca gaaatgtttt taagtccgat ttctttatat ttactttttt
    31381 gagtgcgaat ctaaaaatat gatgaagcta gtttgatcaa tttcactaga actgaatgaa
    31441 gtgaaagtat tatcgttgaa tcaaacgcga ccacccctat attagtaaat catttatttt
    31501 attaacgttt atacataatt gttagagagg aataaaagtc cttatcatta gttgttaaac
    31561 aacactttaa agactagaaa tgagtcagaa aatactagta atatatagag aacccagtca
    31621 atgtcatata cagcgattta taaatattgt aaaatatgat agatacacga atcgtaaagg
    31681 agagagacaa gtggaggata agaattcagt tattgtattt aaaaatgttt catttcaata
    31741 tcaaagtgat gcatccttca cattgaaaga tgtttctttt aatataccta aaggtcagtg
    31801 gacatctatt gttggtcata acggttctgg aaaatctaca attgccaagt taatgattgg
    31861 catagagaaa gttaaatctg gagaaatttt ttataataat caagctataa ctgatgataa
    31921 ttttgaaaag ttaagaaaag acataggaat tgtatttcag aatccggata atcaatttgt
    31981 tggttcaatt gtaaaatacg atgtggcatt tggactcgaa aatcatgcgg ttccatatga
    32041 cgaaatgcat agaagagtca gcgaagcact taaacaagtt gatatgttag aacgtgcaga
    32101 ttatgaacct aatgcattat cggggggaca gaagcagcgt gtggctatag caagtgtatt
    32161 agcacttaac ccctctgtca ttatattaga tgaggcgact tctatgttag atcctgatgc
    32221 acgtcaaaat ttattggatt tagtgagaaa agttaaatca gaacataata ttacaatcat
    32281 ttctattacg catgatttat ctgaggcgat ggaagcagat catgttatcg ttatgaataa
    32341 agggactgtc tataaagaag gcacagcgac tgaaattttc gacaatgcag aagagttaac
    32401 aagaataggt ctagatttgc cattcccaat caaaataaat caaatgctgg gacaccaaac
    32461 atcattctta acttatgaag ggctggtgga tcaactatga ctatacggtt tgacaatgta
    32521 agttatacct atcaaaaagg gacaccatat cagcatcaag ctattcatga tgttaataca
    32581 gaatttgaac aaggtaaata ttacgccatc gttggacaaa cgggtagtgg taaatcaacg
    32641 ttgatacaaa atattaatgc gctgttaaag ccgactactg ggacagttac agttgatgac
    32701 attactatta cacataagac caaagataaa tatattagac ctgtaagaaa aagaattgga
    32761 atggtatttc aatttcccga atctcaatta tttgaggaca cagtagagcg tgaaatgata
    32821 tttggaccta aaaactttaa aatgaattta gatgaagcca aaaactatgc ccatcgtctg
    32881 ttgatggatt taggcttttc aagagatgta atgtcacaat caccatttca aatgtcaggt
    32941 ggacaaatgc gtaaaatagc gattgtatcg atattggcaa tgaatcctga tattatcgtg
    33001 gttgatgaac ctacagcagg acttgatcca caaagtaaac gacaagtaat gagattacta
    33061 aagtcactac aaacagatga aaataaggca attatcctaa tttcacatga tatgaatgaa
    33121 gtcgcgcgtt atgcagatga agtcatcgtt atgaaagaag gtagtatagt gtcgcaaaca
    33181 tcaccaaaag agctcttcaa agataaaaaa aaattggcgg attggcatat tggtttgcca
    33241 gaaattgttc agttacaata tgactttgaa caaaaatatc aaacaaaatt aaaagatatt
    33301 gccttaactg aagaagcatt tgtaagcttg tataaggagt ggcaacatga aaaataaatt
    33361 gattataggg cgatatttac caattaattc ttttgttcat catcttgatc caagagcaaa
    33421 gttaatgttt gtttttcttt ttattatatt aatatttttc tgtcattcac cattaacata
    33481 tctttgggtg tttgcactta tcttattcat tatgagatta gcaaaaattc aattatggtt
    33541 cctaatcaaa ggtttaacac caatattttt ctttttaatc ttcacattaa tgatgcatat
    33601 atttttaact aaaggtggat atgtattagt tgaatggcat ggcatcacga ttgaaactaa
    33661 tggtatttta gaagggctat atatatcact gcgtttaatt gggattgtaa tgattgcaac
    33721 aattatgaca ctatctacaa gtccaattga tttaacagat gcgtttgaaa gattacttgc
    33781 accactaaag atgtttaaat taccagttca tcaattaagt atgataatgt ccattgcgtt
    33841 acgattcatc ccgacgttaa tggatgagtt agataaaatc attttggcgc aaaagtcgcg
    33901 tggttctgaa ataagttcag ggaacattgc aacacgtatt aaatcattta ttccattact
    33961 agtgccacta ttcatctcag ctttccaacg cgccgaagaa ttagcggtcg caatggaagt
    34021 taggggttat gatgccaatg ttaagagaac gagctacaga cagcttaaat ggcaattgag
    34081 agatacgata tctttaacca tgattatccc aattgcaatt attttattcg tattaaaata
    34141 ttcaggagtg taaattcatg cgtatattag tagaaattgc gtatcaagga aataattttc
    34201 taggttttca aattcaacaa aatggtcgaa cggtacagca acaatttgaa aagctattac
    34261 aacgcatgca taaaagacat gtgagaatcc atccttcaag taggactgat agaggtgtac
    34321 atgcgataca gcaatacttt catttcgaca cagagctgaa tatacctatg tcacaatggc
    34381 aatatgcaat gaaccgaacg ttacctgatg atatttatgt caacaatgta gttacagtcg
    34441 atgacgattt tcattgtcgt tatgattgtg taggtaaacg ttatcgctac aaagtatatc
    34501 aagcacaaca ccgcgatcca tttcaaagtg gtttgaaaac atttattcct gaaacgttgg
    34561 atttaggtaa aatgaacaga gcggcgcaac aattcatagg tacacatgat tttacgggat
    34621 tttgttcgca aaaaactgaa gtagaaagca aagttagaac attataccaa agtgaaatcg
    34681 taaagacaga tgatggcttt gattatattg tcactggatc tggattttta tataacatgg
    34741 tgcgtgtctt agttgcattt ttaatagaag ttggaaaagg gcgacatgaa gtatctgatg
    34801 taccaaaact tcttgaatca aagaatcgta agaatgtacc tttcactgca ccggctgaag
    34861 gattatattt agagaaaatt tatttagatg aaaatgagtt attaaaagac tttggtaacg
    34921 atatcaaaat acatcgtaaa aaatcattac aaaatgactg attgccattg acaaaaagct
    34981 tgtgaaatta taagattatt aacggtattg ttttatatcc accccacgat aagccccgga
    35041 aacttattgt gttacaagat atataagcag aaacgaacaa cagttaacaa aataaatgaa
    35101 attaaacgtt ttaaaaatga aacaaatgaa atcatctatt aggttatgaa actgtttata
    35161 gcttgaatag aagcatttat tttttaggag gacaattatt atgcgtcaaa catttatggc
    35221 aaatgaatca aacattgagc gcaaatggta tgttatcgat gctgaaggcc aaacattagg
    35281 tcgtttatca tcagaagtag catctatctt acgcggtaaa aataaagtaa cttacacacc
    35341 acacgttgat actggtgatt atgtaatcgt tattaatgca tcaaaaatcg aatttactgg
    35401 taacaaagaa actgacaaag tttactaccg tcactcaaat cacccaggtg gtatcaaatc
    35461 aatcactgct ggtgaattaa gaagaactaa cccagaacgt ttaattgaaa actcaattaa
    35521 aggtatgtta ccaagcactc gtttaggcga aaaacaaggt aaaaaattat ttgtatatgg
    35581 tggcgctgaa catccacacg ctgcacaaca accagaaaac tacgaattac gtggttaatt
    35641 agaaggagga aatgactttg gcacaagttg aatatagagg cacaggccgt cgtaaaaact
    35701 cagtagcacg tgtacgttta gtaccaggtg aaggtaacat cacagttaat aaccgtgacg
    35761 tacgcgaata cttaccattc gaatcattaa ttttagactt aaaccaacca tttgatgtaa
    35821 ctgaaactaa aggtaactat gatgttttag ttaacgttca tggtggtggt ttcactggac
    35881 aagctcaagc tatccgtcac ggaatcgctc gtgcattatt agaagcagat cctgaataca
    35941 gaggttcttt aaaacgcgct ggattactta ctcgtgaccc acgtatgaaa gaacgtaaaa
    36001 aaccaggtct taaagcagct cgtcgttcac ctcaattctc aaaacgttaa ttgtcggacg
    36061 atatatacaa aacacctcga tattatgtcg aggtgttttt ttgcgttttt gcggcgaata
    36121 tggaatgtgt agaatataaa tgaattttta ccttcccacc ataaaagatg aagaaccatg
    36181 aaatgtggag aacaataaat agttgatatt ctgttatttt tttggaagtg aagtgatttg
    36241 aatactttac tctaaacgat aaaaggttaa aaaaacaaca aaaagaaaat attactgtca
    36301 taattggaaa tacaccgtat tctgctggtc aatctagtgt aaataatgat aataaaaaaa
    36361 tgaatatgaa aaattaaatg caaaaatagc aaatacctat gcaaattttt caacagcaaa
    36421 tttaaaaaaa tcattatatg attcgtatat acaggctttt cgatgggcaa ctgatagagt
    36481 tggaaaaaag gtattatagg atttgtaaca aatggatcat ttattgatag tcaaagcact
    36541 gatggattta gaaaagtttt atacgatgaa tttaactatc tgtacataat taatttgaga
    36601 ggcgaccaac gaacacaggg tgaaaaatca agaaaagaag gtggaaaaat atttggatca
    36661 ggaagtagag caccgatagc tatatctatt ttagttaaag atggaagtta caatcacgat
    36721 atctattata atgacattgg tgaatattta actagagagc aaaaattaga tactttaatg
    36781 aaacatcaat caattgtgaa tttgaaaagt ttgaatgttt tacccgataa aaacaatgat
    36841 tggattaatc aacgagacat aaactatgag aattatttac caatgtatga ttccaaggat
    36901 attgaaaatt ctatatattt agatcaattt aatggagtaa attcagcaag agataattgg
    36961 gtaacaaatt tttcgaatga aaaagcatta gtaaatgcaa aattacttgt agataactat
    37021 aattctgaaa tagatagatt aattgatatc ttagattcaa gagaacgtat aaatttagtg
    37081 aacaaagatg aaacatttat tagttggaca cgaggactta cccaaaaatt ttctaaagga
    37141 aaaaatattt caattaatcc agagagaatt gttaaattta tgcatagacc atttacaaaa
    37201 aaatggatag tgtacgacaa aaatattatg gaaatgccaa gtagatatta taatataatg
    37261 gagaatactg gacaggtgat ttatatacaa ggacagggaa tgaataaaga attctcggct
    37321 atgatcacgg acatattacc taatttccaa tttattggta atggcaaagg ctttgctaca
    37381 tacaaaggta aagatagttt gaggcttgta gataatattt ctaattcttt taaaaagaaa
    37441 ataaatctta attcagaaga gatagtatat tatatatatg caattttgca tcataaatat
    37501 tatgtgaaca aatattcaag tgatttgagt aaaggttttc cgcgaatacc gatattaaag
    37561 gatgtatatg gatttgttga aattggtagg gagttagtgg aattacattt aaattatgaa
    37621 aaacaactca attgggatgg tgtagaaata atttataata atatgaatcc gaattataaa
    37681 gttgaaaaat gaagcaccct aaaaagggcg tgttggatac agtaatatac aataccgata
    37741 ttacagttac taacattcca aataaagtat ataattatat tgtgaatggc aagcctgcta
    37801 ttgaatggat tatagatcag tacaacgtaa gcatcgacaa gaaatctgga atattagatg
    37861 atcctaatga atttagtgaa gatccgaatt acattttaaa tctgctttta tcagttatta
    37921 ctgtaagtat gaaaactctt ggattaatag ataaattacc agattttgaa gtattagatt
    37981 aaggagagga tatattgaca aatactatca aaaaaataat ttattttgat gaactatcag
    38041 caatggattt attacagata gagaagaaag gtaaccttac taaaacagtt cagttgatgc
    38101 atgatgaaaa aggtagtgca agtggtgaat ctaaagtgaa catggaattt ggtaagcaaa
    38161 gcgctgtaaa acaaattttt gattctttac ttgggatgtc agcgagtgct tcggctagtg
    38221 caggtataaa tggaaatata actggagagc gagtagccaa aacaatttta gagaatacat
    38281 taatatatga ttttttggat acagtagagt ttagaaagaa aaaaactatt attgatattt
    38341 cagaggggtt taaactaaat attcaacaag atacgatgac atatttcgcg actattaccc
    38401 ccatagcaag tatgtttgaa ggatctaaac atctagacga ggaggagcta tcatttgatg
    38461 tttcaaaaat gaatgaagcg attagaagta ttaaaggcta ttttgaatta ataggaacaa
    38521 gagggaatga aaacagagtt tttagattta atatagaatc ttttaaaaat aactatagaa
    38581 tacaagattt aaaatttatg aatttaacac tctactcagt acttgttggt gaaataagca
    38641 aagaaaatct aaattacgat aaagagtttg atctaaatca aggtgccaaa cctagtggaa
    38701 ttggaaagtt taagggaatt agatcagaag caactaaaga agataaatat caagtgtatg
    38761 atgttattct tgcaggagtg aaataaatgg aaattcatgt ttatacggga ccgctttgtg
    38821 gatttaatga atatattaat tctgaagtta aagaatatca ccgcttcatg gaattaatca
    38881 gagaatacaa tatcatagtg cgagcaagtg atacatttgg aactcatgat gttgatggta
    38941 tttttgatac tgagatagaa aatcttgtga tattttcaaa cgactttgct tctgtaacaa
    39001 ctcatgttat tactaatttt ataaatatcg ttactttagg tagaaatatt aagaaaatct
    39061 atattcagaa tcctccaaaa agagtatttc aat
//
LOCUS       DAKTIO010000005        39040 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.005, whole genome shotgun sequence.
ACCESSION   DAKTIO010000005 DAKTIO010000000
VERSION     DAKTIO010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 39040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39040
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.005"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            304..2349
                     /locus_tag="O0293_000176"
     CDS             304..2349
                     /locus_tag="O0293_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HCY0279089.1"
                     /translation="MRKKLFGQLQRIGKALMLPVAILPAAGLLLAIGTAMQGESLQHY
                     LPFIQNGGVQTVAKLMTGAGGIIFDNLPMIFALGVAIGLAGGDGVAAIAAFVGYIIMN
                     KTMGDFLQVTPKNIGDPASGYASILGIPTLQTGVFGGIIIGALAAWCYNKFYNINLPS
                     YLGFFAGKRFVPIMMATTSFILAFPMALIWPTIQSGLNAFSTGLLDSNTGVAVFLFGF
                     IKRLLIPFGLHHIFHAPFWFEFGSWKNAAGEIIHGDQRIFIEQIREGAHLTAGKFMQG
                     EFPVMMFGLPAAALAIYHTAKPENKKVVAGLMGSAALTSFLTGITEPLEFSFLFVAPL
                     LFFIHAVLDGLSFLTLYLLDLHLGYTFSGGFIDYFLLGILPNKTQWWLVIPVGLVYAV
                     IYYFVFRFLIVKLKYKTPGREDKQSQAATASATELPYAVLEAMGGKANIKHLDACITR
                     LRVEVNDKSKVDVPGLKDLGASGVLEVGNNMQAIFGPKSDQIKHEMQQIMNGQVVENP
                     TTMEDDKDETVVVAEDKSATSELSHIVHAPLTGEVTPLSEVPDQVFSEKMMGDGIAIK
                     PSQGEVRAPFNGKVQMIFPTKHAIGLVSDSGLELLIHIGLDTVKLNGEGFTLHVEEGQ
                     EVKQGDLLINFDLDYIRNHAKSDITPIIVTQGNITNLDFKQGEHGNISFGDQLFEAK"
     gene            complement(2328..2429)
                     /locus_tag="O0293_000177"
     CDS             complement(2328..2429)
                     /locus_tag="O0293_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279090.1"
                     /translation="MMLQNTIAKKKQARHLMKVYAPVYSKHYLASNN"
     gene            2623..4263
                     /locus_tag="O0293_000178"
     CDS             2623..4263
                     /locus_tag="O0293_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-keto acid decarboxylase family protein"
                     /protein_id="HCY0279091.1"
                     /translation="MKQRIGAYLIDAIHRAGVDKIFGVPGDFNLAFLDDIISNPNVDW
                     VGNTNELNASYAADGYARLNGLAALVTTFGVGELSAVNGIAGSYAERIPVIAITGAPT
                     RAVEQGGKYVHHSLGEGTFDDYRKMFAHITVAQGYITPENATTEIPRLINTAIAERRP
                     VHLHLPIDVAISEIEIPTPFEVTATKYTDASTYIELLATKLHQAKQPIIITGHEINSF
                     HLHQELEDFVNQTQIPVAQLSLGKGAFNEENPYYMGIYDGKIAEDKIRDYVDNSDLIL
                     NIGAKLTDSATAGFSYQFNIDDVVMLNHHNIKIDDVTNDEISLPSLLKQLSNISYTNN
                     ATFPAYHRPTSPDYTVGTEPLTQQTYFKMMQNFLKPNDVIIADQGTSFFGAYDLALYK
                     NNTFIGQPLWGSIGYTLPATLGSQLADKDRRNLLLIGDGSLQLTVQAISTMIRQHIKP
                     VLFVINNDGYTVERLIHGMYEPYNEIHMWDYKALPAVFGGKNVEIHDVESSKDLQDTF
                     NAINGHPDVMHFVEVKMAVEDAPKKLIDIAKAFSQQNK"
     gene            4330..4887
                     /locus_tag="O0293_000179"
     CDS             4330..4887
                     /locus_tag="O0293_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCY0279092.1"
                     /translation="MINFNKTALVLIDLQEGILKMDYAPYTAENVVQNANKLIDVFRK
                     NNGFIAFVRVNFYDGKDVLQPNAMISLPPKEGDDYSRFHHLLDKRDDDFVIDKRHFSA
                     FVGTDLDLQLRRRGIDTIVLGGVATHIGVDTTARDAYQLNYNQFFVTDMMSAQNETLH
                     QFPIDNVFPLMGQTITTNDFLNILN"
     gene            5163..6518
                     /gene="brnQ"
                     /locus_tag="O0293_000180"
     CDS             5163..6518
                     /gene="brnQ"
                     /locus_tag="O0293_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCY0279093.1"
                     /translation="MKKKLTFKENMFIGSMLFGLFFGAGNLIFPIHLGQAAGSNVFIA
                     NLGFLITAIGLPFLGIIAIGISKTSGLFEIASRVNKTYAYIFTIALYLVIGPFFALPR
                     LATTSFEIAFSPFLSPKQITLYLFIFSFVFFVIAWFFARKPSRILEYIGKFLNPVFLV
                     LLAIILLFAFIHPLGGISDAPISKQYQSHALFNGFLDGYNTLDALASLAFGIIIVATI
                     KKLGIENPTDIAKETIKSGTISIIMMGIIYTLLAIMGTLSIGHFKLSENGGIALAQIT
                     QYYLGNYGIVLLSLIVMVACLKTAIGLITAFSETFEHLFPKMNYLAIATVVSFISFLF
                     ANVGLTKIIMYSVPVLMFLYPLAIALIVLTLFSSKFHHSKLIYQCTIFFTMIAALVDG
                     LKASPEFISSTSFSQTLINFSQKYLPLSDIGMGWVVLSLIGFIIGFIIYKIKHRKIPQ
                     A"
     gene            6771..7955
                     /gene="rocD"
                     /locus_tag="O0293_000181"
     CDS             6771..7955
                     /gene="rocD"
                     /locus_tag="O0293_000181"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HCY0279094.1"
                     /translation="MNSIIELTDYYSSNNYAPLKLVISKGKGVKVWDTDGKQYIDCIS
                     GFSVANQGHCHPTIVKAMTEQASKLSIISRVLYSDNLGKWEEKICHLAKKDKVLPLNS
                     GTEAVEAAIKIARKWGSEVKGITDGQVEIIAMNNNFHGRTLGSLSLSNHDAYKAGFHP
                     LLQGTTTVDFGDIEQLTQAISPNTAAIILEPIQGEGGVNIPPKGYIQAVRQLCDKHQI
                     LLIADEIQVGLGRTGKWFAMEWEQVVPDIYILGKALGGGLYPVSAVLANNDVMRVLTP
                     GTHGSTFGGNPLAIAISTAALDVLKDEQLVERSERLGSFLLKALLQLKHPSIKEIRGR
                     GLFIGIELNTDAAPFVDQLIQRGILCKDTHRTIIRLSPPLVIDKEEIHQIVAAFQDVF
                     KN"
     gene            7994..9025
                     /locus_tag="O0293_000182"
     CDS             7994..9025
                     /locus_tag="O0293_000182"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCY0279095.1"
                     /translation="MIKVGIVGGSGYGAIELIRLLQTHPHVTIAHIYSHSKVDEPLKL
                     TFPHLQHIMQHFEALTVDNNDCDVIFFATPAPVSKTCIPPLVEKGIHVIDLSGAFRIK
                     NREIYEAYYKETAAAQDDLNHAIYSISEWQSFDNNGTKLISNPGCFPTATLLALHPLI
                     SEKIVDLSSIIIDAKTGVSGAGRSLSQRVHFSEMNENLSAYAIGNHKHKPEIEQYLSI
                     IAGQDVSVIFTPHLVPMTRGILSTIYVKLSSEYTTESLHKLMTSYYANQPFVRIRDIG
                     TFPTTKEVLGSNYCDIGIYVDETTQTAILVSVIDNLVKGASGQAIQNLNILYDFEVTT
                     GLNQSPVYP"
     gene            9037..10278
                     /gene="argJ"
                     /locus_tag="O0293_000183"
     CDS             9037..10278
                     /gene="argJ"
                     /locus_tag="O0293_000183"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HCY0279096.1"
                     /translation="MKHQETTSQQYNFSIIKHGDISTPQGFTAGGMHIGLRANKKDFG
                     WIYSSSLASAAAVYTLNQFKAAPLIVTEDTLQKSKGKLQALVVNSANANSCTGQQGID
                     DARQTQTWVAQQLQIPSEHVAVASTGVIGEYLPMDKIKTGTEHIKDANFATPGAFNEA
                     ILTTDTCTKHIAVSLKIDGKTVTIGGSTKGSGMIHPNMATMLAFITTDASIESNTLHQ
                     LLKSSTDHTFNMITVDGDTSTNDMVLVMANHQVEHQILSQDHPQWETFVDAFNFVCTF
                     LAKAIARDGEGATKLISVNVSGAKSISDARKIGKTIVSSNLVKSAIFGEDANFGRIIT
                     AIGYSGCEIDPNCTYVQLNQIPVVDKGMAVLFDEQAMSNTLTHENVTIDVQLGLGNAA
                     ATAYGCDLSYDYVRINASYRT"
     gene            10294..11058
                     /gene="argB"
                     /locus_tag="O0293_000184"
     CDS             10294..11058
                     /gene="argB"
                     /locus_tag="O0293_000184"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCY0279097.1"
                     /translation="MKFIVIKIGGSTLSDMHPSIINNIKHLRSNNIYPIIVHGGGPFI
                     NEALSNQQIEPHFVNGLRVTDKATMTITKHTLIADVNTALVAQFNQHQCSAIGLCGLD
                     AQLFEITSFDQQYGYVGVPTALNKDALQYLCTKFVPIINSIGFNNHDGEFYNINADTL
                     AYFIASSLKAPIYVLSNIAGVLINDVVIPQLPLVDIHQYIEHGDIYGGMIPKVLDAKN
                     AIENGCPKVIIASGNKPNIIESIYNNDFVGTTILNS"
     gene            11328..11807
                     /locus_tag="O0293_000185"
     CDS             11328..11807
                     /locus_tag="O0293_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HCY0279098.1"
                     /translation="MGIFIYAGIIGGLLSGIVKLGWEVMFPPRTPERNATNPPQELLQ
                     QLGFSSEFTHQTYTFSNMELPWVSFIVHFSFSIVIAIIYCILVKKYAYLAMGQGAVFG
                     IAIWVLFHLIIMPIMHTVPAVWDQPFQEHLSEFFGHIVWMMTIELVRQHFVYRYKLN"
     gene            complement(12135..12779)
                     /locus_tag="O0293_000186"
     CDS             complement(12135..12779)
                     /locus_tag="O0293_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCY0279099.1"
                     /translation="MTVFVMQLQSNLKSIEELISQSRWSYKKPRTVNYRYNQDKLMHR
                     LGDILVQYGIQHDTGLLPHEWHYHISPRGKADIVQHNRDGQPIYVSLSYSYPYIVCVV
                     DKEPVGIDIEKISQRLDWRTLVTCFSTNEAHQICSLNDFYQIWTQKESFTKLIGEGLI
                     KGLDIYDMTQSHFYQSREVKFKQFIFDQFMVQVCFLGQAPWGYKKVSVFQLLSS"
     gene            complement(12792..19967)
                     /locus_tag="O0293_000187"
     CDS             complement(12792..19967)
                     /locus_tag="O0293_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HCY0279100.1"
                     /translation="MIMGNLRFQQEYFRIYKNNTESTTHRNAYWVKLAKNVEATKMMY
                     ALSTIVQQHASIRHFFDVTTDDNLTMILHEFLPFIEIKQVPSSSANYDLEAFFKQELS
                     TYHFNDSPLFKVKLFQFADAAYILLDFHVSIFDDSQIDIFLDDLCNAYRGNTVINNTR
                     QHAHINRNDDKDNQDASHIALDSNYFRLENNSDIHIDSYFPIKHPFEQALYQTYLIDD
                     MTSIDMASLAVSVYLANHIMSQQHDVTLGIHVPSHLPNDLHGNIVPLTLTIDAKDVCQ
                     RFTTDFNKCVLQNMSQLQCAKSSLSLETIFHCYHHMMSCCNDVIEDVHQIHDAHTSLA
                     DIEIFPHQHGFKIIYNSAAYDLLSIETLSDLVRNIYLQITEENGNKRTTVDELNLMTE
                     RDIQLYDDINLSLPEIDDAQTVVTLFEQQVEATPNHVAVQFDGVFITYQTLNARANDL
                     AHRLRNQYGVEPNDRVAVIAEKSIEMIIAMIGVLKAGGAYVPIDPNYPSDRQEYILKD
                     VTPKVVITYQALYENGKQNINHIDLNKIAWKNIDNLSKCNTLEDHAYVIYTSGTTGNP
                     KGTLIPHRGIVRLVHQNHYVPLNEETTILLSGTIAFDAATFEIYGALLNGGKLIVAKK
                     EQLLNPIAVEQLINENDVNTMWLTSSLFNQIASERIEVLVPLKYLLIGGEVLNAKWVD
                     LLNQKPKHPQIINGYGPTENTTFTTTYNIPNKVPNRIPIGKPILGTHVYIMQGERRCG
                     VGIPGELCTSGFGLAAGYLNQPELTADKFIKDSNINQLMYRSGDIVRLLPDGNIDYLY
                     RKDKQVKIRGFRIELSEVEHALERIQGINKAVVIVQNHDQDQYIVAYYEAMHTLSHNK
                     IKSQLRMTLPEYMIPVNFMHIEQIPITINGKLDKKALPIMDYVDTDAYVAPSTDTEHL
                     LCQIFADILHVNQVGIHDNFFELGGHSLKATLVVNRIEASTGKRLQIGDLLQKPTVFE
                     LAQAIAKVQEQNYEVIPETIVKDDYVLSSAQKRMYLLWKSNHKDTVYNVPFLWRLSSE
                     LNVAQLRQAVQRLIARHEILRTQYIVVDDEVRQRIVADVAVDFEEVNTHFTDEQEIMR
                     QFVAPFNLEKPSQIRVRYIRSPLHAYLFIDTHHIINDGMSNIQLMNDLNALYQHKLLL
                     PLKLQYKDYSEWMSHRDMTKHRQYWLSQFKDEVPILSLPTDYVRPNIKTTNGAMMSFT
                     MNQQMRQLLQKYVEKHQITDFMFFMSVVMTLLSRYARKDDVVVGSVMSARMHKGTEQM
                     LGMFANTLVYRGQPSPDKMWTQFLQEVKEMSLEAYEHQEYPFECLVNDLDQSHDASRN
                     PLFDVMLVLQNNETNHAHFGHSKLTHIQPKSVTAKFDLSFIIEEDRDDYTINIEYNTD
                     LYHSETVRHMGNQCMIMIDYILKHQDTLQICDIPNGTEELLNWVNTHVNDRMLNVPGN
                     KSIISYFNEVVSRQGNHVALVMNDLTMTYETLRNYVDAIAHMLLSNGVGNGQRVALFT
                     ERSFEMIAAMLATVKVGASYIPIDIDFPNKRQGAILEDAKVTAVMSYGVEIETTLPVI
                     QLENAKGFVESKENEQYDDLHGNQLENTAMLDNEMYAIYTSGTTGMPKGVAIRQRNLL
                     NLVHAWSTELQLGDNEVFLQHANIVFDASVMEIYCCLLNGHTLVIPDREERVNPEQLQ
                     QLINKHRVTVASIPLQMCSVMEDFYIEKLITGGATSTASFVKYIEKHCGTYFNAYGPS
                     ESTVITSYWSHHCGDLIPETIPIGKPLSNIQVYIMSDGLLCGIGMPGELCIAGDSLAI
                     GYINRPELMADKWQNNPFGKGKLYHSGDLARYTSDGQIEFLGRIDKQVKVNGYRIELD
                     EIENAILAIRGISDCVVTVSHFDTHDILNAYYVGEQQVEQDLKQYLNDQLPKYMIPKT
                     ITHIDCMPLTTNDKVDTTRLPNPSPIQQSNKVYSEPSNEIEQTFVDVFGEVLKQNDVG
                     VDDDFFELGGNSLEAMLVVSHLKRFGHHISMQTLYQYKTVRQIVNYMYQNQQSLVALP
                     DNLSELQKIVMSRYNLGILEDSLSHRPLGNTLLTGATGFLGAYLIEVLQGYSHRIYCF
                     IRADNEEIAWYKLMTNLNDYFSEETVEIMLSNIEVIVGDFECMDDVVLPENMDTIIHA
                     GARTDHFGDDDEFEKVNVQGTVDVIRLAQQHHARLIYVSTISVGTYFDIDTEDVTFSE
                     ADVYKGQLLTSPYTRSKFYSELKVLEAVNNGLDGRIVRVGNLTNPYNGRWHMRNIKTN
                     RFSMVMNDLLQLDCIGVSMAEMPVDFSFVDTTARQIVALAQVNTPQIIYHVLSPNKMP
                     VKSLLECVKRKEIELVSDESFNEILQKQDMYETIGLTSVDREQQLAMIDTTLTLKIMN
                     HISEKWPTITNNWLYHWAQYIKTIFNK"
     gene            complement(20414..21664)
                     /locus_tag="O0293_000188"
     CDS             complement(20414..21664)
                     /locus_tag="O0293_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0279101.1"
                     /translation="MKRLSTTLKVRLISNFLQLIITTAFIPFIALYLTDMLSQSIVGI
                     YLVGLVVLKFPLSIISGYLIEIFPKKLLVLIYQATMVIMLVFMGVFGSHQLWQIIGFC
                     VAYAIFTIVWGLQFPVMDTLIMDAITEDVEHYIYKISYWMTNLSVAIGALLGGLMYGY
                     SMLLLFLIAACIFLIVLFILYIWLPQDRNQVKQSDDKRHASRYQKLQIMNIFRSYKLV
                     LKDRNYMLLISGFSIIMMGEFSISSYIAIRLKDQFETISIGSYDITGAKMLAILLMIN
                     TVVVILLTYSISKVVLKIDFKKALITGLLIYIVGYSGLTYLNQFGLLVVFMIIATVGE
                     IIYSPIVSEQRFKIIPKAKRGTYSAVNALGIHFSETLARLGIVLGVFLTSLQMGLYMF
                     IVLTIGASMLVAGVFGGQKQVNTN"
     gene            complement(22050..23075)
                     /locus_tag="O0293_000189"
     CDS             complement(22050..23075)
                     /locus_tag="O0293_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HCY0279102.1"
                     /translation="MKIVALFPEAVEGQENQLLNTKKALGLKTFLEERGHEFIILADN
                     GEDLDKHLPDMDVIISAPFYPAYMTRERIEKAPNLKLAITAGVGSDHVDLAAASEHNI
                     GVVEVTGSNTVSVAEHAVMDLLILLRNYEEGHRQSVEGEWNLSQVGNHAHELQHKTIG
                     IFGFGRIGQLVAERLAPFNVTLQHYDPINQQDHKLSKFVSFDELVSTSDAITIHAPLT
                     PETDNLFDKDVLSRMKKHSYLVNTARGKIVNRDALVEALASEHLQGYAGDVWYPQPAP
                     ADHPWRTMPRNAMTVHYSGMTLEAQKRIEDGVKDILERFFNHEPFQDKDIIVASGRIA
                     SKSYTAK"
     gene            complement(23248..23607)
                     /locus_tag="O0293_000190"
     CDS             complement(23248..23607)
                     /locus_tag="O0293_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HCY0279103.1"
                     /translation="MNTIDTHTKEQQFSNLVRSYRKEYVGKGPNSIRVSFKDNWAIAH
                     MTGVLSKVESFYLNDKRNESMLHYTRTEKIKQMYKEIDVNEMESLVGAKFVKLFTDID
                     LNDDEVISIFVFDKSIE"
     gene            complement(23824..24855)
                     /locus_tag="O0293_000191"
     CDS             complement(23824..24855)
                     /locus_tag="O0293_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HCY0279104.1"
                     /translation="MTLETLIKEQLDPHLVEVDEGTYYPRTFIQQLFVDGYFGEAALR
                     KNAEVIEAVSQSCLTTGFCLWCQLAFSTYLENATQPHLNNDLQQQLLSGEILGATGLS
                     NPMKSFNDLEKLNLEHTYVDGQLVVSGRMPAVSNIQEDHYFGAISKHESSDEFVMFIL
                     RANQDGITLVEKTNFLGVNGSATYQITLNQVVVPQSQIITHDAKQFAATIRPQFIAYQ
                     IPIGLGSIKSSLELIDAFSNVQNGINQYLEYDVEAFKKRYRQLREEYYAILDDGNLTS
                     HLNELISLKKDIGYLLLDVNQASVVNGGSRAYTPYSPQVRKLKEGFFFAALTPTLRHL
                     GKLEAELKG"
     gene            complement(24868..25629)
                     /locus_tag="O0293_000192"
     CDS             complement(24868..25629)
                     /locus_tag="O0293_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0279105.1"
                     /translation="MTRPTNNKFILPIITFIIFLGIWEMVIIIGHYQPVLLPGPALVG
                     KSIWSFIVTGEIFQHLAISLWRFVAGFVVALLVAIPLGFLLGRNRWLYNAIEPLFQLI
                     RPISPIAWAPFVVLWFGIGSLPAIAIIFIAAFFPIVFNTIKGVRDIEPQYLKIAANLN
                     LTGWSLYRNILFPGAFKQIMAGIHMAVGTSWIFLVSGEMIGAQSGLGFLIVDARNMLN
                     LEDVLAAIFFIGLFGFIIDRFISYIEQFILRRFGE"
     gene            complement(25626..26600)
                     /locus_tag="O0293_000193"
     CDS             complement(25626..26600)
                     /locus_tag="O0293_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0279106.1"
                     /translation="MKRLSIIVIIGIFIITGCDWQRTSKERSKNAQNQQVIKIGYLPI
                     THSANLMMTKKLLSQYNHPKYKLELVKFNNWPDLMDALNSGRIDGASTLIELAMKSKQ
                     KGSNIKAVALGHHEGNVIMGQKGMHLNEFNNNGDDYHFGIPHRYSTHYLLLEELRKQL
                     KIKPGHFSYHEMSPAEMPAALSEHRITGYSVAEPFGALGEKLGKGKTLKHGDDVIPDA
                     YCCVLVLRGELLDQHKDVAQAFVQDYKKSGFKMNDRKQSVDIMTHHFKQSRDVLTQSA
                     AWTSYGDLTIKPSGYQEITTLVKQHHLFNPPAYDDFVEPSLYKEASRS"
     gene            complement(26614..27354)
                     /locus_tag="O0293_000194"
     CDS             complement(26614..27354)
                     /locus_tag="O0293_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0279107.1"
                     /translation="MIKIQQLQHHFGSHKVIHNFNLDISKGEIVTFIGKSGCGKSTLL
                     NIIGGFIHPSSGRVIIDNEIKQQPSPDCLMLFQHHNLLPWKTINDNIRIGLQQKISDE
                     EINAQLKLVDLEDRGKHFPEQLSGGMKQRVALCRAHVHKPNVISMDEPLGALDAFTRY
                     KLQDQLVQLKHKTQSTIILVTHDIDEAIYLSDRIVLLGEGCNIISQYEITASHPRSRN
                     DSHLLKIRNEIMETFALNHHQVEPEYYL"
     gene            complement(27696..28208)
                     /locus_tag="O0293_000195"
     CDS             complement(27696..28208)
                     /locus_tag="O0293_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HCY0279108.1"
                     /translation="MTLFLLEANNLDFASTKEELEAKAASLSTKTIPTLIEVQATENL
                     THGYFIVEANDEAEAKQFLTEADISIQLVKEVRLVGKDLDEVKNGDAHVDYLVTWNIP
                     EGITMDQYLARKKKNSVHYEEVPEVEFKRTYVCEDMSKCICLYNAPDEEAVRRARKAV
                     DTPIDGIEKL"
     gene            28387..28590
                     /locus_tag="O0293_000196"
     CDS             28387..28590
                     /locus_tag="O0293_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279109.1"
                     /translation="MDKESVVASLARNKKIAVETMAGQRYIIERILHTNDEKHIHILK
                     PKDVVLDVDSIKEIDENHLNDAT"
     gene            complement(28652..29611)
                     /locus_tag="O0293_000197"
     CDS             complement(28652..29611)
                     /locus_tag="O0293_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCY0279110.1"
                     /translation="MNGKKANTINRYKYFHHVNHQKIQQSSKKTLWASLIITLLFTVI
                     EFVGGLVSNSLALLSDSFHMLSDVLALGLSMLAIYFASKKPTARYTFGYLRFEILAAF
                     LNGLALIVISIWILYEAIVRIIYPQPIESGIMFMIASIGLLVNIILTVILVRSLKQED
                     NINIQSALWHFMGDLLNSIGVIVAVVLIYFTGWRIIDPIISIVISLIILRGGYKITRN
                     AWLILMESVPQHLDTDQIMADIKNIDGILDVHEFHLWSITTEHYSLSAHVVLDKKYEG
                     DDYQAIDQVSSLLKEKYGIAHSTLQIENLQLNPLDEPYFDKLT"
     gene            complement(30257..31744)
                     /locus_tag="O0293_000198"
     CDS             complement(30257..31744)
                     /locus_tag="O0293_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCY0279111.1"
                     /translation="MAVNVRDYIAENYGLFINGEFVKGSSDETIEVTNPATGETLSHI
                     TRAKDKDVDHAVKVAQEAFESWSLTSKSERAQMLRDIGDKLMAQKDKIAMIETLNNGK
                     PIRETTAIDIPFAARHFHYFASVIETEEGTVNDIDKDTMSIVRHEPIGVVGAVVAWNF
                     PMLLAAWKIAPAIAAGNTIVIQPSSSTPLSLLEVAKIFQEVLPKGVVNILTGKGSESG
                     NAIFNHDGVDKLSFTGSTDVGYQVAEAAAKHLVPATLELGGKSANIILDDANLDLAVE
                     GIQLGILFNQGEVCSAGSRLLVHEKIYDQLVPRLQEAFSNIKVGNPQDEATQMGSQTG
                     KDQLDKIQSYIDAAKESDAQILAGGHRLTENGLDKGFFFEPTLIAVPDNHHKLAQEEI
                     FGPVLTVIKVKDDQEAIDIANDSEYGLAGGVFSQNITRALNIAKAVRTGRIWINTYNQ
                     VPEGAPFGGYKKSGIGRETYKGALSNYQQVKNIYIDTSNALKGLY"
     gene            32171..32554
                     /locus_tag="O0293_000199"
     CDS             32171..32554
                     /locus_tag="O0293_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HCY0279112.1"
                     /translation="MRLILIVIGLIFTALGIAGAVLPLLPTTPFLLVAVFCFARSSDR
                     FYNWLINQKIYKEYVENFYLHRGYTLQQKIKILISLYIVIGFSIYMVDVLAVRVGLII
                     MVIIQTAVLFTFVKTLPKSNHKIEE"
     gene            32561..32887
                     /gene="isdI"
                     /locus_tag="O0293_000200"
     CDS             32561..32887
                     /gene="isdI"
                     /locus_tag="O0293_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdI"
                     /protein_id="HCY0279113.1"
                     /translation="MFMAENRLQLQKGSAEETIERFYNRQGIETIEGFQQMFVTKTLN
                     TEDTDEVKILTIWESEDSFNNWLNSDVFKEAHKNVRLKSDDDGQQSPILSNKVFKYDI
                     GYHYQK"
     gene            complement(32952..34097)
                     /gene="cap8P"
                     /locus_tag="O0293_000201"
     CDS             complement(32952..34097)
                     /gene="cap8P"
                     /locus_tag="O0293_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8P"
                     /protein_id="HCY0279114.1"
                     /translation="MKKIMVIFGTRPEAIKMAPLVKEIDHNGNFEANIVITAQHRDML
                     DSVLSIFDIQADHDLNIMQDQQTLAGLTANALAKLDSIINEEQPDMILVHGDTTTTFV
                     GSLAAFYHQIPVGHVEAGLRTHQKYSPFPEELNRVMVSNIAELNFAPTVIAAKNLLFE
                     NKDKERIFITGNTVIDALSTTVQNDFVSTIINKHKGKKVVLLTAHRRENIGEPMHQIF
                     KAVRDLADEYKDVVFIYPMHRNPKVRAIAEKYLSGRNRIELIEPLDAIEFHNFTNQSY
                     LVLTDSGGIQEEAPTFGKPVLVLRNHTERPEGVEAGTSRVIGTDYDNIVRNVKQLIED
                     DEAYQRMSQANNPYGDGQASRRICEAIEYYFGLRTDKPDEFVPLRHK"
     gene            complement(34174..35436)
                     /gene="cap8O"
                     /locus_tag="O0293_000202"
     CDS             complement(34174..35436)
                     /gene="cap8O"
                     /locus_tag="O0293_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8O"
                     /protein_id="HCY0279115.1"
                     /translation="MKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQSGQI
                     SIEEPGLQEVYEEVLSSGKLKVSTTPDASDVFIIAVPTPNNDDQYRSCDISLVMRALD
                     SILSFLEKGNTIIVESTIAPKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILE
                     ELVHNNRIIGGVTEACIEAGKRVYRTFVQGEMIETDARTAEMSKLMENTYRDVNIALA
                     NELTKICNNLNINVLDVIEMANKHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKL
                     IQTGREINNSMPAYVVDTTKQIIKVLSGNKVTVFGLTYKGDVDDIRESPAFDIYELLN
                     QEPDIEVCAYDPHVELDFVEHDMSHAVKDASLVLILSDHSEFKNLSDSHFDKMKHKVI
                     FDTKNVVKSSFEDVSYYNYGNIFNFIDK"
     gene            complement(35490..36377)
                     /gene="capN"
                     /locus_tag="O0293_000203"
     CDS             complement(35490..36377)
                     /gene="capN"
                     /locus_tag="O0293_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     epimerase CapN"
                     /protein_id="HCY0279116.1"
                     /translation="MRKNILITGVHGYIGNALKDKLIEQGHQVDQINVRNQLWKSTSF
                     KDYDVLIHTAALVHNNSPQARLSDYMQVNMLLTKQLAQKAKAEDVKQFIFMSTMAVYG
                     KEGHVGKSDQVDTQTPMNPTTNYGISKKFAEQALQELISDSFKVAIVRPPMIYGAHCP
                     GNFQRLMQLSKRLPIIPNINNQRSALYIKHLTAFIDQLISLEVTGVYHPQDSFYFDTS
                     SVMYEIRRQSHRKTVLINMPSMLNKYFNKLSVFRKLFGNLIYSNTLYENNNALEIIPG
                     KMSLVIADIMDETTTKDKA"
     gene            complement(36377..36934)
                     /gene="cap8M"
                     /locus_tag="O0293_000204"
     CDS             complement(36377..36934)
                     /gene="cap8M"
                     /locus_tag="O0293_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8M"
                     /protein_id="HCY0279117.1"
                     /translation="MKRLFDVVSSIYGLVVLSPILLITALLIKMESPGPAIFKQKRPT
                     INNELFNIYKFRSMKIDTPNVATDLMDSTSYITKTGKVIRKTSIDELPQLLNVLKGEM
                     SIVGPRPALYNQYELIEKRTKANVHTIRPGVTGLAQVMGRDDITDDQKVAYDHYYLTH
                     QSMMLDMYIIYKTIKNIVTSEGVHH"
     gene            complement(36945..38150)
                     /gene="cap8L"
                     /locus_tag="O0293_000205"
     CDS             complement(36945..38150)
                     /gene="cap8L"
                     /locus_tag="O0293_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8L"
                     /protein_id="HCY0279118.1"
                     /translation="MSEKKILILCQYFYPEYVSSATLPTQLAEDLIANHINVDVMCGW
                     PYEYSNHKQVSKTEMHRGIRIRRLKYSRFNNKSKVGRIINFFSLFSKFVINIPKMLKY
                     DQILVYSNPPILPLIPDVLHRLLKKKYSFVVYDIAPDNAIKTGATRPGSMIDKLMRYI
                     NRHVYKNAENVIVLGTEMKNYLLNHQISKNADNIHVIPNWYDMRQLQDNRIYNDTFKA
                     YREQYDKILLYSGNMGQLQDMETLISFLKLNKDQSQTLTILCGHGKKFADVKTAIEDH
                     RIENVKMFEFLTGTDYADVLKIADVCIASLIKEGVGLGVPSKNYGYLAAKKALVLIMD
                     KQSDIVQHVEQYDAGIQIDNGDAHAIYNFINTHSSKELHEMGERAHQLFKDKYTREIN
                     TMKYYNLLK"
     gene            complement(38151..>39040)
                     /locus_tag="O0293_000206"
     CDS             complement(38151..>39040)
                     /locus_tag="O0293_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HCY0279119.1"
                     /translation="LNIIDIIFLILLNILMCLRIYLNVFFRMTLKYNQILYIALIQFL
                     GLLIGLFLYYLTQNWIVCFITSELFATIYTLVKLRGLTIGEYQSEDNNVVKDYVMLLS
                     TNSLNNLNLYLDRLILLPIIGGTAVTISFLSTFIGKMLATFLYPINNVVLSYISVNES
                     DNIKKQYLKTNLIAIAALCLVMIICYPITIIIVSLLYNIDSSLYSKFIILGNIGVLFN
                     AVSIMIQTLNTKHASITLQANYMTLHTITFIFITILMTIAFGLNGFFWTTLFSNIIKY
                     VILNIIGLKSKFINKKDVD"
BASE COUNT        12614 a         7314 c         6029 g        13083 t
ORIGIN      
        1 ttaagagatt tgaaattgaa tttaataatt gtattgaaaa cgcatacttc accatgctaa
       61 aataggagtc gcaaacaaat aagattcaat aagatgtgat ggtaagctag ataaactaac
      121 gtatcagttt tatatactac ttagtcaatt gcttctttta cattaattac atacaccaac
      181 gtgttactaa gtaagattag gcatgagtta aattaagctg tgatggttac caacacagtc
      241 tatttgctcg tgtctttttt tattgaatct taaataataa atacaacttt ggaggttgga
      301 caagtgagga agaaactttt cggtcaattg caacgtattg gtaaagcgct aatgttacct
      361 gttgcgattt taccagcagc tggtctgtta ttagctatcg gtacagctat gcaaggtgaa
      421 tcattacaac actacttgcc gtttatacaa aatggtggcg tacaaactgt cgctaaatta
      481 atgacaggtg ctggtggtat catttttgat aacttgccta tgattttcgc attaggtgtc
      541 gcaatcggat tagctggcgg tgatggcgta gcagctatcg cagcattcgt cggttacata
      601 atcatgaaca aaacaatggg cgacttttta caagttacac ctaagaatat tggtgatcca
      661 gcgagtggtt acgctagcat tttaggtatc ccaacattac aaacaggtgt gttcggcggt
      721 attataatcg gggccctggc agcttggtgt tataacaagt tctataacat taacttacca
      781 tcttatttag gtttcttcgc tggtaagcgt ttcgtaccta ttatgatggc tacaacatca
      841 tttattttag cattcccaat ggcattaatt tggccaacga ttcaatcagg attaaatgca
      901 ttcagtacag gattattaga ttcaaatact ggtgttgccg tattcttatt tggtttcatc
      961 aagcgtttat taattccatt cggtctacat cacattttcc acgcaccgtt ctggttcgag
     1021 tttggttcat ggaaaaatgc agctggtgaa attattcacg gtgaccaacg tatctttatc
     1081 gaacaaattc gtgaaggcgc acatttgaca gctggtaaat tcatgcaagg tgaattccct
     1141 gttatgatgt tcggtttacc tgcagcagct ttagcaattt atcacacagc taaacctgaa
     1201 aataagaaag tagtagcagg tttaatgggt tctgctgctt taacatcatt cttaactggt
     1261 attacagaac cattagaatt ctcattctta tttgtagcac cattattatt ctttattcac
     1321 gcagtacttg atggtttatc attcttaaca ttgtacttat tagatcttca tctaggttat
     1381 acattctcag gtggtttcat cgactacttc ttactcggta tactacctaa taagacacaa
     1441 tggtggttag tcattcctgt aggtcttgta tacgcagtta tttactactt cgtattccga
     1501 ttcttaattg taaaattaaa atacaaaaca ccaggtcgtg aagataaaca atcacaagcg
     1561 gctactgctt cagcaactga attaccatat gcagtattag aagctatggg tggcaaagca
     1621 aacattaaac atttagacgc ttgtatcaca cgtctacgtg ttgaagttaa cgacaaatct
     1681 aaagttgatg ttcctggttt gaaagattta ggcgcatctg gtgtattaga agtcggcaat
     1741 aatatgcaag caatttttgg tcctaaatct gaccaaatca aacatgaaat gcaacagatt
     1801 atgaatggtc aagtagtaga aaatcctact actatggaag acgataaaga cgaaactgtt
     1861 gttgttgcag aagataaatc tgcaacaagc gaattgagcc atatcgtgca tgcaccatta
     1921 actggtgaag taacaccatt atcagaagtg cctgatcaag tgttcagcga aaaaatgatg
     1981 ggtgacggta tcgctatcaa accttcacaa ggtgaagttc gtgcaccatt caacggtaaa
     2041 gtacaaatga ttttcccaac aaaacatgca attggtcttg tatcagatag tggtttagaa
     2101 ctattaatcc acatcggttt agacactgtt aaattaaacg gagaaggctt tactttacat
     2161 gttgaggaag gtcaagaagt taaacaaggt gatttattaa tcaactttga tttagactac
     2221 atccgcaatc atgcaaagag tgatattacg cctattatcg tgacacaagg aaacattaca
     2281 aaccttgatt ttaaacaagg tgaacatggc aacatttcat ttggcgatca attatttgaa
     2341 gctaaataat gcttactata aacaggtgcg tataccttca taaggtgacg cgcctgtttt
     2401 ttctttgcta ttgtattttg cagcatcatt gatagttcgc tctcccctta aattttgaat
     2461 tttaagatca tcaattaaag ccccccttca tactcatttc ctaaaaaata ttaattgttc
     2521 actattgtta gcgttttcac aacaaagtca acttccttga ccttacacta tattcgaggc
     2581 tatcatttta agtgtaaata tagagaaaag gtggcttttt ttatgaaaca acgcattgga
     2641 gcttacttaa ttgacgctat tcatcgagca ggcgtcgata aaatttttgg tgttcctggt
     2701 gattttaatc tcgcttttct agacgatatt atcagcaatc ccaatgtaga ttgggtagga
     2761 aatacaaatg aattaaacgc aagttacgca gcggacggtt atgcccgtct taatggactc
     2821 gctgcattag ttactacatt tggtgttggc gaattaagtg ccgtcaacgg tatcgcaggt
     2881 tcatatgctg aacgcatacc tgtcattgcg attacaggtg cgccgacacg tgctgttgaa
     2941 caaggcggta aatatgtaca tcactcactt ggtgaaggta catttgacga ctatcgaaaa
     3001 atgtttgcac atataaccgt tgcacaaggt tatatcacac ctgaaaatgc aacaaccgaa
     3061 ataccacgtt taattaatac agcaatcgcc gaaagacgcc cagttcattt acatttacca
     3121 atcgatgtcg caatctctga aattgagata ccgacaccat ttgaagtgac ggcaactaaa
     3181 tatacggatg catcaacata tatagagtta ttagcaacta aactgcatca agcgaagcag
     3241 cctatcatca ttactggaca tgaaattaac agttttcacc tccatcaaga attagaagat
     3301 tttgtaaatc aaacacagat accagtagca caactttcat taggaaaagg tgcttttaat
     3361 gaggaaaatc catattatat gggtatttac gatgggaaaa ttgccgaaga taaaatacga
     3421 gattatgtgg acaacagcga tttaatttta aatattggag ccaaattaac agattcagca
     3481 acagcaggtt tttcatacca attcaatatc gatgatgtcg ttatgttaaa tcatcacaat
     3541 atcaaaattg acgatgttac aaatgatgaa atatctctac catcattgtt aaaacagtta
     3601 tccaatattt catatacgaa taacgcaacg ttccctgcgt atcatcgtcc aacatcaccc
     3661 gattatactg ttggcacaga accattaaca caacaaactt attttaaaat gatgcaaaat
     3721 ttcttaaaac caaatgatgt catcattgct gatcaaggta catcattctt tggtgcttat
     3781 gatttagcat tatacaaaaa caatactttt atagggcaac cgttatgggg ttctatcggc
     3841 tatacattac ctgcaacatt aggttcacaa ttagcagaca aagatcgtcg taacttatta
     3901 ttaattggtg atggctcatt gcaactaact gttcaagcta tttcaactat gattagacag
     3961 catattaaac cggtattatt tgtgattaat aatgacggct atacggtaga acgacttatt
     4021 cacggcatgt atgaacctta taatgaaatt cacatgtggg attataaagc tttaccagct
     4081 gtatttggtg gtaaaaatgt tgaaattcat gacgttgaat catcaaaaga tttacaagac
     4141 acgtttaatg caattaatgg tcatcccgat gtgatgcatt ttgtcgaagt caaaatggct
     4201 gtcgaagacg caccgaagaa actcatcgat atcgctaaag ctttttcaca acaaaataaa
     4261 taatttcatc gtatacaggg tataagttta agcgaatact ttattaaacg aataggactc
     4321 tgatataaga tgattaattt taataaaacc gctttagtgt taatcgacct gcaagaaggt
     4381 attcttaaaa tggattatgc cccatataca gctgaaaatg tcgttcaaaa cgctaataaa
     4441 ttaatagatg tttttagaaa aaacaatggc tttatcgctt ttgttcgcgt gaatttctat
     4501 gatggtaaag atgtattgca accaaatgca atgatctcat taccaccaaa agaaggcgac
     4561 gactacagtc gtttccatca tttattagac aagagagatg acgattttgt catagacaaa
     4621 cgacatttta gtgcatttgt aggaacagat ttggacttac aattgcgacg tcgaggaatt
     4681 gatacgattg ttcttggtgg tgtcgcaacg catattggcg tagatacgac agcgcgagat
     4741 gcctatcaat taaactacaa tcagtttttt gttacagata tgatgagtgc acaaaacgaa
     4801 acgctacatc aatttccaat agataatgta ttcccattga tgggacaaac aataactaca
     4861 aacgactttc taaatatatt gaactaaaca tatacttccc cccttcgatc atgttgaggg
     4921 ggatctttat ttcacaaagt attaatacgt cgggttgtct aaccttctat atttaacata
     4981 ttctatatct gttaaatcgt tcttaactta cgcccctact acataaaaaa cagtatttat
     5041 tccggaattt tcaaaaaatt tagtatttat tgcaaaatta tgtatcactt tatgtttaat
     5101 ttttgatatt atcttaatta agtagatttt tataagttct aaaaaggaga acaaatacat
     5161 atatgaagaa gaaactaaca tttaaagaaa acatgtttat aggttctatg ttatttggtt
     5221 tattctttgg tgccggcaat cttatcttcc caatacactt gggtcaagct gctggttcta
     5281 acgtttttat cgctaactta ggatttttaa ttacagcaat tggcttacca tttctaggta
     5341 tcattgctat tggcatttca aagacatctg gtttatttga aattgcatcg cgtgttaata
     5401 aaacatatgc ttacattttc acgattgcct tatatctagt tatcggacca tttttcgcct
     5461 tacctagact ggcaacgaca tcatttgaaa ttgcattttc gccattttta tcaccaaagc
     5521 aaatcacttt atatttattt atttttagct tcgtcttctt tgtgattgca tggttttttg
     5581 cgagaaagcc atcaagaatt ttagaatata tcggtaaatt tttaaatccg gtattcttag
     5641 tattattagc aattatttta ttatttgctt ttatccatcc attaggtggc atatctgatg
     5701 cacctattag taaacaatat caatcacatg ccttatttaa cggcttttta gatggataca
     5761 ataccttaga tgcgctagcg tcattggcat ttggtattat cattgttgca acgattaaaa
     5821 agttaggtat cgaaaatcca actgatatcg ctaaagaaac aattaagtct ggtactatca
     5881 gtatcattat gatggggatc atttataccc tactagcaat catgggtaca ttaagtattg
     5941 gtcatttcaa acttagtgaa aatggtggta ttgccttagc gcaaattact caatactact
     6001 taggtaacta cggtatcgtc ctgttgtcac ttatcgttat ggttgcttgt ttaaaaacag
     6061 ccatcggttt gattacggca ttttcagaaa cattcgaaca ccttttccct aaaatgaatt
     6121 acctagcgat tgcaacagtt gtaagcttta tttcgttctt attcgcgaat gttggtttaa
     6181 ctaagattat tatgtactca gtcccagtgt taatgttctt atatccatta gcaattgcct
     6241 tgattgtact aacattattt agtagcaaat tccatcattc aaaacttatt tatcaatgta
     6301 ccattttctt tacaatgatt gctgcattag tagatggatt aaaagctagt ccagagttca
     6361 tttcaagcac atcattctca caaactttga ttaatttcag ccaaaaatat ttaccattat
     6421 cagacattgg tatgggctgg gttgttctca gtttgattgg tttcattatc ggcttcatta
     6481 tttataaaat taagcatcgt aaaattccac aagcataata ctatgccaca gtcatatgtt
     6541 aaacatatgc ttgtggcatt ttttattcat actacattaa actgcaatcg tatacataca
     6601 tatcaatgat tatccacaaa aaatattagt actttcattt tacaaatcac attaatacaa
     6661 acacaacctt atctttatat tattaaattt acatttgaca cttatattga acaactgtaa
     6721 tatattaata ttaattcttt aaaatgtata aatataaagg agggagaccg atgaattcaa
     6781 tcattgaatt aactgattat tatagctcta ataattatgc accacttaag cttgtcattt
     6841 ctaaaggtaa aggtgtcaaa gtttgggata ctgatggcaa acaatatata gattgcattt
     6901 cgggtttttc agttgcaaac caaggccatt gtcatccaac aattgttaaa gcgatgacag
     6961 aacaagcttc aaagttgtct atcatttcac gtgtccttta tagtgacaat ctcgggaaat
     7021 gggaagaaaa aatttgtcat cttgctaaga aagacaaagt actccccctt aactctggta
     7081 ctgaagctgt tgaagcagcc attaaaattg ctagaaaatg gggctctgaa gttaaaggca
     7141 ttactgacgg acaagttgaa atcatcgcta tgaataacaa ttttcacggt cgtacacttg
     7201 gctcattatc actatctaac cacgacgcat ataaagcagg atttcacccc ctacttcaag
     7261 gcactacaac agtagatttt ggagacattg aacaattaac acaagctatt tcaccgaata
     7321 cagcagcaat tattttggaa ccaattcaag gtgaaggtgg cgttaatata ccaccgaaag
     7381 gatatattca agctgtgcgt caactatgtg ataaacatca aatattattg attgcagatg
     7441 aaattcaagt tggtcttggt agaactggga aatggtttgc tatggaatgg gagcaagtcg
     7501 ttccagacat ttatatttta ggtaaggcat tgggtggcgg cttataccct gtatctgctg
     7561 tacttgcaaa taatgatgtc atgcgtgttc taacaccagg tacacatggt tcaacatttg
     7621 gtggtaaccc tttagccatt gcaatatcga cggcagcgct tgatgtactt aaagatgaac
     7681 aactggttga acgatcagaa cgcttaggtt catttttatt aaaagcgttg ctacaactta
     7741 aacatcctag tattaaagaa attagaggtc gtggtttatt tataggcata gagcttaaca
     7801 cagatgctgc accttttgtg gatcaactga ttcaacgtgg aatcttatgc aaagacacgc
     7861 atcgtactat cattcgattg tctccacctc tagtcattga taaagaggaa atccatcaaa
     7921 ttgttgcagc ttttcaagac gtttttaaaa attaacaatt aatcatttat atatgacata
     7981 ggagggattc atgatgatta aagtaggtat cgttggcggt agcggttatg gcgcaattga
     8041 attaattcga ttgttacaaa cacatcctca tgtaacgatt gcacacatct actcacattc
     8101 aaaagtagat gaaccgttga aattaacatt tccacattta caacatatta tgcaacattt
     8161 cgaagcactt acagtggaca ataatgactg tgatgtaatt ttctttgcga caccagcacc
     8221 cgtaagtaaa acatgtatcc ctcccttagt agaaaaaggt attcatgtta tcgatttatc
     8281 tggcgcattt agaattaaga atcgtgaaat atatgaagca tattacaaag aaactgctgc
     8341 agcacaagat gatttgaatc atgctattta cagcatttca gaatggcaat cgtttgataa
     8401 caatggaacg aagctcattt ctaatcctgg ctgtttccct acagcaacat tattagcatt
     8461 acatccactt attagcgaaa aaatagtaga tttgtcatct attattattg atgctaagac
     8521 cggcgtgtca ggtgctggtc gttcattatc acaacgtgtt catttttcag aaatgaatga
     8581 aaatctaagc gcttatgcaa tcggaaacca taaacacaaa ccggaaatcg agcaatattt
     8641 atctatcatt gcgggtcaag atgtatcagt catatttaca ccacatctcg taccaatgac
     8701 acgaggtatt ttatcaacaa tatatgtcaa attatcatct gaatatacga ctgaatcatt
     8761 acataaatta atgacctctt attatgctaa tcagccattt gtcagaatta gagatattgg
     8821 gacttttcca accacaaaag aagtactcgg tagtaactac tgcgatatcg gcatctatgt
     8881 agatgaaaca acgcaaacag caattttagt atcagtgatt gataaccttg tcaaaggcgc
     8941 aagtgggcaa gccattcaaa atttaaatat attatatgat tttgaagtga cgactggcct
     9001 aaatcaatca ccagtttatc cataaggggt gttagaatga aacatcaaga aacgacatca
     9061 caacaatata acttttcaat tattaaacat ggcgatatca gtacacctca aggcttcacg
     9121 gctggtggta tgcacatcgg tttacgcgct aacaaaaaag actttggatg gatttactca
     9181 tcgtctttgg caagtgcagc tgccgtatat actttaaatc agtttaaagc tgcaccactt
     9241 attgtcactg aagacacttt acaaaagtct aaaggaaaat tacaagcact tgttgttaat
     9301 tcagctaatg caaattcttg taccggtcaa caaggcatag atgatgcacg acaaacacaa
     9361 acatgggttg ctcaacaact tcaaatacca tctgagcatg ttgctgttgc ttcaactggg
     9421 gtcattggtg aatatttgcc tatggataaa attaagactg ggaccgaaca tattaaggat
     9481 gctaattttg caacgccagg tgcgtttaac gaggcaattt taacaactga tacctgtaca
     9541 aaacatatcg ctgtatcact aaaaatcgat ggtaaaaccg ttacaattgg tggtagcacc
     9601 aaaggttcag gtatgattca cccaaatatg gctaccatgc ttgcttttat aacaaccgat
     9661 gcatcgattg aatcgaatac acttcatcaa ttattaaaat cttcgactga ccatacattt
     9721 aatatgatta ctgttgatgg cgatacaagt acaaatgaca tggtattagt catggcaaat
     9781 caccaagttg aacaccaaat acttagtcaa gaccatccac aatgggaaac atttgttgat
     9841 gcattcaatt ttgtctgtac atttttagct aaagctatag ccagagatgg cgaaggcgca
     9901 acaaagttaa tcagcgtcaa tgtgtcaggc gctaaatcaa tatcagatgc aagaaaaatc
     9961 ggtaaaacta tcgtaagttc aaatctagta aaatcagcta tttttggcga agatgccaat
    10021 tttggtcgaa tcattacagc tattggctac agcggatgtg aaattgatcc taactgcaca
    10081 tatgttcaac tgaaccaaat acctgtcgtt gataaaggta tggctgtact atttgatgag
    10141 caagctatgt cgaatacatt aactcatgaa aatgtcacaa ttgacgttca gcttggttta
    10201 ggtaacgctg cagcgactgc atacggttgt gatttatcct atgattatgt gcgtatcaac
    10261 gcatcatatc gaacataagg tggtgttggt tagatgaaat ttattgtcat taaaattggt
    10321 ggcagtacac ttagtgacat gcatccatca attattaaca acattaagca tttacgatca
    10381 aacaacatct accccattat cgttcatggc ggtggcccat ttattaatga agcattatca
    10441 aaccagcaaa tcgagccaca ctttgttaat ggcctaagag tgactgataa agcaaccatg
    10501 accattacta aacacacgct cattgcagac gttaacactg cattagtagc tcaatttaac
    10561 cagcaccaat gttctgcaat aggcttatgt ggtttggatg cacagctgtt tgaaattaca
    10621 tcttttgatc aacaatatgg atatgtcggt gttccgaccg ctttaaataa ggatgcttta
    10681 cagtatttat gtactaaatt tgtacctatc atcaattcga ttggtttcaa taaccatgat
    10741 ggagaatttt acaatattaa tgctgacacg cttgcctatt ttattgcatc atcattaaaa
    10801 gcgcctattt atgtattaag taatattgca ggtgtactca tcaatgatgt tgttatacct
    10861 caattgccat tagtcgatat tcatcaatat attgaacatg gtgatattta tggaggtatg
    10921 attcccaaag tgctagatgc caaaaatgcg attgaaaatg gctgtcctaa agttatcatt
    10981 gcatcaggaa acaagccaaa tatcattgaa tctatttaca ataatgattt tgttggcaca
    11041 acaatcctta attcataact atgaaattaa ggcctaacaa gttttgacac gcgagatgat
    11101 tccagttcga ttatccattg cgctaaaaca tttatttacc gttcatctcg ttaacaattt
    11161 tgaatacagt acgatacaat atgagatgta aaaaactaat aaccttttac aaatttgttt
    11221 atcaaaatat tttaagtttt gcaaagcttt ttattgtgat tattttcaca aaatactata
    11281 atgaggatag taaatagaga ggagtcctta agttgacgaa acgacaaatg ggtatattca
    11341 tttatgctgg aattatcggt ggcttgttat ctggaattgt aaaattaggt tgggaggtca
    11401 tgtttccacc tcgcacacca gaacgtaatg caacgaaccc acctcaagag ttattgcaac
    11461 aattaggatt tagtagtgag tttacgcatc aaacatatac attttcaaat atggaattgc
    11521 cttgggtaag ctttattgtc cactttagtt tttctatcgt cattgcaatt atttactgca
    11581 tattagttaa aaaatacgct tacttagcaa tgggacaagg tgctgttttt ggtattgcta
    11641 tttgggtatt attccacctt atcattatgc caatcatgca tactgtacct gctgtgtggg
    11701 atcaaccatt ccaagagcat ctgtcagaat tctttggcca tatcgtctgg atgatgacaa
    11761 ttgaattagt gcgacaacat tttgtctatc gctataaatt aaattaatac actgactaac
    11821 attaacgtga gttttaaatc atcgtttgag tatgatgatt gatgctcacg ttattttatt
    11881 aactgacatg atatgattcc agccaactta cgtgagcatt aaagtctcaa atgcgtcgta
    11941 acaaactatt attttcggta atttcaatat tgctcagtat atttttacct tatcacttac
    12001 tttaatctcg tcatgatttt gaatgatgcc atcgtgtatt cacctttcat ttttccaata
    12061 aaaaaacatc taacagtaaa catttaggca gtatagttta aaatcactgc gcaatgatac
    12121 tgtcagacgt catattaact actcaataac tgaaatacag acactttttt ataaccccag
    12181 ggtgcctgtc ctaagaaaca tacctgtacc ataaactgat caaaaataaa ttgtttgaac
    12241 ttcacttcac gtgattgata aaagtgtgat tgtgtcatat cataaatgtc caatcctttg
    12301 attaaacctt caccaatcaa ttttgtaaaa ctttcttttt gtgtccatat ttgataaaaa
    12361 tcatttaaac tacatatttg atgtgcttcg tttgtagaga aacacgtcac taacgtacgc
    12421 cagtctaaac gttgtgatat cttttcgata tcaataccaa ctggttcttt atcgacaaca
    12481 cacacgatat aaggataact atatgataag ctcacataga tgggctgtcc atcacgattg
    12541 tgttgaacaa tatctgcctt acctcgtggc gaaatgtgat aatgccattc atgtggtaat
    12601 aaacctgtgt catgttgaat tccatattgc actaaaatat ctcccaatct gtgcatgagt
    12661 ttatcttgat tgtatctata gttgactgta cgcggttttt tatatgacca acgactttgt
    12721 gatattaatt cttcaatact tttcaagtta ctctgtaatt gcattacaaa tactgtcata
    12781 actttcccta cttacttatt gaatattgtt ttgatatatt gtgcccaatg atacagccaa
    12841 ttgttagtta tcgttggcca tttttcactg atgtgattca ttatttttaa tgttaatgtt
    12901 gtatctatca ttgctagttg ttgttcacgg tcaacactag ttaatccaat cgtttcgtac
    12961 atgtcttgtt tctgtaaaat ttcattaaat gattcatcgc tgacgagttc aatttctttg
    13021 cgcttaacgc attctaacaa agatttcacc ggcattttat taggtgatag cacatggtaa
    13081 atgatttgtg gtgtgttgac ctgtgctaat gcgacaattt gtcttgcagt cgtatccaca
    13141 aaagaaaaat ctacaggcat ttcagccatg ctaaccccga tacaatccag ttgtaacaaa
    13201 tcattcatta ccattgaaaa acggttagtc tttatatttc tcatatgcca tcttccattg
    13261 taaggattcg tcaaattacc aacacgtaca atccgaccat ctaagccatt atttacagct
    13321 tctaatactt ttaattcact ataaaatttg ctccgtgtat atggtgatgt tagtagttgc
    13381 cctttataga catccgcttc tgaaaatgtc acatcttctg tgtctatatc aaaataagtt
    13441 cccacactta tcgtagacac atatattaac cttgcatgat gttgttgtgc caaacgtatg
    13501 acatcaacag taccttgaac atttactttt tcaaattcat catcatcacc aaagtgatct
    13561 gtacgagcac ctgcatgaat aatcgtatcc atgttttctg gtaaaacaac atcatccata
    13621 cactcgaaat caccaacaat gacttcaatg tttgataaca ttatttcaac cgtctcttct
    13681 gaaaaataat catttaaatt cgtcatcaac ttataccatg ctatttcctc attatcagca
    13741 cgtatgaaac aataaatgcg atgactgtat ccttgtagta cttcaatcag ataagcacct
    13801 aaaaaacctg tcgcgccagt caatagtgta tttcctagag gtcgatgact tagactatcc
    13861 tctaaaatac ccaagttata acgagacata acaatctttt gtaattccga aagattatcc
    13921 ggtaatgcaa ctaatgattg ttgattttgg tacatataat taacaatctg tcgcacggtt
    13981 ttatattggt ataatgtctg cattgaaata tgatggccaa atcgttttaa atgcgagaca
    14041 actaacatcg cctctaatga gttaccacca agttcaaaga aatcatcgtc aacaccgaca
    14101 tcattttgtt tcaatacctc tccaaataca tcaacaaatg tctgctcaat ttcattagag
    14161 ggttcgctat acactttatt agactgttgt ataggtgatg gatttggcaa acgcgtagta
    14221 tccaccttgt cattcgtggt taatggcata caatcgatat gcgttatagt cttaggaatc
    14281 atatacttag gcagctgatc atttaaatat tgcttcaaat cctgttccac ttgttgctct
    14341 ccgacataat aagcattcaa tatatcatgc gtatcaaagt gacttactgt tacaacacaa
    14401 tcagatatac cacgaatagc taatattgca ttttcaattt catcaagttc aatacggtac
    14461 ccgttaactt tcacttgttt atctattctt cctaaaaatt caatttgacc atcagatgta
    14521 taacgtgcta aatcaccact atgatacaac tttcctttac caaatggatt attttgccat
    14581 ttatcagcca ttaattctgg acgattaata tatcctatcg ctaaactatc acctgcaata
    14641 cacaactcgc ctggcatacc aataccgcat aacaaaccat ctgacataat atacacttgg
    14701 atgttagata agggtttgcc aattggaatc gtctcaggta tcaaatcacc acaatgatgt
    14761 gaccaatacg atgtgatgac tgttgactca gatggtccat aggcattgaa atacgtgcca
    14821 caatgcttct caatatattt aacaaaggat gccgtactag ttgccccgcc tgtaatcaac
    14881 ttttcaatat aaaagtcttc cataacacta cacatctgta acggaatcga cgcaaccgtc
    14941 acacgatgct tattaatgag ttgttgtaac tgttctggat taacacgttc ctctctatct
    15001 ggaatcacaa gcgtatgacc atttaacaaa caacaataaa tctccataac tgatgcatca
    15061 aaaacaatat ttgcatgttg caaaaatact tcattgtcgc ctaattgcaa ttcagttgac
    15121 catgcatgca ctaaattcaa caaatttcgt tgtcgtatgg caaccccttt aggcatcccg
    15181 gtcgtaccag atgtgtaaat agcatacatc tcattatcta acatcgctgt gttttcaagt
    15241 tgattgccat gtaaatcatc atattgttca ttttcctttg attcaacaaa gcctttagca
    15301 ttttccaatt gaatgactgg taatgtcgtt tcaatttcaa cgccgtaaga catgactgca
    15361 gttactttag catcctccaa aattgcacct tgtcgtttat tcggaaaatc aatatcgata
    15421 ggtatataag atgcacctac tttaactgtc gccaacatcg ccgcaatcat ttcaaaacta
    15481 cgttctgtaa acaaggcaac ccgttgacca ttgcccacac catttgatag gagcatgtgc
    15541 gcaatggcat ccacatagtt gcgtaatgtt tcatacgtca ttgtcaaatc attcatgact
    15601 agcgcaacat gattaccttg tcgtgagaca acttcattaa agtaacttat gatagattta
    15661 tttcccggga cattaagcat tcgatcgtta acatgcgtat tgacccaatt tagaagttcc
    15721 tccgtgccgt ttggtatatc acaaatttgt agtgtatctt gatgcttcaa aatataatca
    15781 atcataatca tacattgatt acccatgtga cgaactgttt ctgagtgata taaatcggta
    15841 ttatactcga tattgattgt atagtcatcg cgatcttctt caatgatgaa agataaatca
    15901 aatttcgccg tcactgattt gggttgaatg tgtgttaatt tactatgccc aaaatgagca
    15961 tgattcgttt cattgttttg tagtactaac atgacatcaa ataatggatt ccgtgaggca
    16021 tcatgtgatt gatctaagtc atttactaaa cattcgaatg ggtattcttg atgctcgtat
    16081 gcctccaaac tcatttcctt aacctcttgt aaaaactgtg tccacatttt atcaggtgac
    16141 ggttgccctc tatataccaa cgtattagca aacatgccta gcatttgctc cgtgccttta
    16201 tgcatacgcg cactcatcac actaccgaca acaacatcat cttttcgagc atatctactt
    16261 aacaacgtca tgaccacact cataaagaac ataaaatcag taatttgatg cttttctaca
    16321 tacttttgaa gtagctgtct catttgttga ttcattgtaa atgacatcat tgctccattt
    16381 gtcgttttaa tatttggtct aacatagtct gtcggtaagc ttaaaatagg tacttcatct
    16441 ttgaattgag ataaccaata ttgtctatgt ttcgtcatat cacgatgcga catccactca
    16501 ctatagtctt tatattgcaa tttaagtggt aacaataatt tatgttgata aagtgcgtta
    16561 agatcattca ttaattgtat attactcata ccgtcattaa tgatatgatg cgtatctata
    16621 aagaggtatg catgtaaggg acttctaatg tatctcactc taatttgact tggcttttcc
    16681 aaattaaaag gtgctacaaa ttggcgcatg atttcttgtt catccgtaaa atgcgtgtta
    16741 acttcttcaa agtcaactgc aacatctgcc acaatacgtt gtcgaacctc atcatctaca
    16801 acaatatatt gtgttcgtaa aatctcatgt cgcgctatca aacgctgcac tgcttgtcgc
    16861 aattgagcta cattaagttc tgatgataac cgccataaaa aaggtacgtt atacaccgta
    16921 tctttatggt ttgatttcca taataaatac atacgctttt gtgcagagct cagcacataa
    16981 tcatctttaa ctatagtttc tggaatcact tcatagtttt gttcttgaac cttagcaatc
    17041 gcttgtgcta gttcaaatac agttggcttt tgtaataaat caccaatttg taatcgtttc
    17101 ccagtagatg cctctatccg attcaccact aacgttgctt ttaatgaatg gccacctaat
    17161 tcaaagaaat tatcatgaat acctacttga ttcacatgta aaatatctgc aaaaatttgg
    17221 catagcaagt gttcggtatc tgtactcggt gctacatagg catccgtatc gacatagtcc
    17281 atgataggca atgccttctt atctaatttc ccattaatag taataggaat ttgctcaata
    17341 tgcatgaaat taactggtat catgtactcc ggtaaggtca tacgtaattg tgatttaatc
    17401 ttattatgtg ataatgtatg catcgcttca taataagcaa cgatatactg atcttgatca
    17461 tgattttgaa caataacaac tgctttatta ataccttgta tacgctcgag cgcatgctca
    17521 acctctgaca actcaatcct aaaccctcga atcttaactt gtttgtcctt tcgatataaa
    17581 taatctatgt tgccatcggg taacaaacga acgatatcac cacttctata catcagctga
    17641 tttatatttg aatctttgat aaatttatct gctgtcaatt ctggctgatt taaataacct
    17701 gcagctaacc caaagccact tgtacataat tctccaggaa taccaacgcc acaccgacgc
    17761 tcgccttgca tgatataaac atgagtaccc agaatcggtt taccaatagg aatacgattt
    17821 ggaactttgt taggtatatt atacgtcgtt gtaaatgttg tattttcagt tggtccataa
    17881 ccattaataa tttgaggatg cttcggtttt tgattaagca aatccaccca cttagcattc
    17941 aatacttctc caccaattaa taaatacttt aacggtacca atacttctat tcgttcacta
    18001 gcaatctgat taaataatga ggaggttaac cacatagtat taacgtcatt ttcattgatt
    18061 aattgttcta ccgctattgg atttaataat tgttcttttt tagcaacaat cagctttcca
    18121 ccattgagca atgcaccata tatttcaaat gttgcagcat caaaggctat agttcctgat
    18181 aacaaaatcg tcgtctcttc atttaatggt acataatgat tttgatggac caatcgaaca
    18241 atacctcggt gcggaattag tgtcccttta gggttaccag ttgtccccga cgtgtaaata
    18301 acataagcat gatcttctaa cgtgttacat ttagaaagat tatcaatatt tttccacgct
    18361 atcttattca aatcaatgtg attaatattt tgtttaccat tttcatataa agcttggtac
    18421 gttattacaa ctttaggcgt tacatctttt aaaatgtact cctgacgatc acttggatag
    18481 ttcggatcaa ttggcacgta agccccacca gctttcaaca cacctatcat cgctattatc
    18541 atctcaatac ttttttcagc tatgacagcg acacgatcat taggttcaac accatactgg
    18601 tttctcaaac ggtgtgctaa atcattcgcg cgtgcattca atgtttgata tgttataaac
    18661 actccgtcaa attgcacagc gacatgattc ggcgttgctt caacttgttg ctcaaataag
    18721 gtaacaactg tttgcgcatc atctatctca ggcaaactta aattgatatc gtcatataat
    18781 tgaatatcac gttctgtcat caaattaagt tcatctacag ttgttcgttt atttccattt
    18841 tcttcagtaa tttgcaaata aatatttcga actaagtcac tcagcgtctc gattgagagc
    18901 aaatcatatg ctgcactgtt atatataatt ttgaacccgt gttgatgtgg aaaaatttca
    18961 atatccgcta aagatgtatg tgcatcatgt atttgatgta catcctcaat aacatcatta
    19021 caacaagaca tcatatgatg ataacaatga aaaatagtct ctagtgaaag cgaagacttc
    19081 gcgcactgta attgcgacat attttgcaac acacatttat taaaatctgt tgtaaaacgt
    19141 tgacatacat cttttgcatc gattgttaac gttaacggca caatatttcc gtgtaaatca
    19201 tttggtaaat gtgatggtac atgtatacct aatgtgacat catgttgttg actcattata
    19261 tgattagcta aatacacact aacagccaac gatgccatat ctattgatgt catatcatca
    19321 atcaaatacg tttgatataa agcttgttca aatggatgct taattggaaa ataactatca
    19381 atatggatgt cagagttatt ctctaaccga aaatagtttg agtctaatgc tatatgcgat
    19441 gcatcttgat tgtctttatc atcatttcta tttatatgtg catgctgtcg agtattgtta
    19501 ataacagtat tgccacgata tgcattgcat aaatcatcaa gaaaaatatc aatttgacta
    19561 tcatcgaaaa tggacacatg aaaatctaat agtatatatg cagcatcagc gaactgaaac
    19621 aatttaactt tgaataaagg tgaatcatta aaatggtaag tacttaattc ttgcttaaaa
    19681 aaagcttcta aatcatagtt tgcggaagaa gatggaactt gttttatctc aataaaaggc
    19741 agaaattcat gaagtatcat tgttaaattg tcatcggtag taacatcaaa aaaatgtctt
    19801 atagatgcat gttgttgcac aattgtcgat aatgcataca tcattttagt agcttcaaca
    19861 tttttagcga gtttaaccca atacgcatta cggtgtgtcg ttgattctgt attatttttg
    19921 tatatacgaa aatattcctg ttgaaatctc aaattaccca taatcataaa aagtccttct
    19981 ttcatatcat aatactcatt acttactgaa attgcatgat gatatgataa ccgacgaaat
    20041 gttaattaac tcgttatgta atgattaata taaaacacca ttcgcaacat atgagcgata
    20101 tattctaccc taaaatacat cttgtatcat cgttacaatt ggtatatttt tcaatgtaaa
    20161 ttacatacat cttcgataaa tagcacacta caactcgtta atcactttct gttgttcaca
    20221 tctcattgca aactcaatat tgttgttaca aaatatccat gaagcaagtt tatattaaac
    20281 aaacaactcg cataaaacaa ttgttatcct taaattttaa caaattctta ataaatttat
    20341 ctctatttta attacgacca aattaatagg ttttccatat aaaaagatgc ataaaataaa
    20401 tatttaaata aattcaattt gtattcactt gtttttgtcc cccaaataca ccagcaacaa
    20461 gcatgctagc accaattgtt aaaacgataa acatatacag tcccatttgt aatgacgtta
    20521 agaaaacacc caacacaatc cctaacctag ctagtgtttc tgaaaaatga atacctaatg
    20581 cattaactgc actatatgtt cctcttttag ctttaggaat aattttaaag cgttgttctg
    20641 aaactatagg cgaataaata atttcaccta cagtcgcaat tatcataaaa acaactaata
    20701 agccaaactg attaagatag gttagaccac tatagccaac aatataaatc agcaaaccag
    20761 tgattaaagc ttttttaaaa tctattttca atacaacttt cgagattgaa tacgtgagta
    20821 aaatgacgac gaccgtatta atcattagca agattgctaa catcttagca cctgtaatat
    20881 catatgaacc tatacttatt gtttcaaact gatcctttag tctaatagca atatatgagg
    20941 agattgaaaa ttcacccatc atgatgatac tgaaccccga aatcaataac atataattac
    21001 ggtctttcaa aactaattta taactgcgaa atatattcat tatttgtaat ttttgataac
    21061 gacttgcatg cctcttgtca tcactttgct ttacttgatt tcggtcttga ggtaaccaaa
    21121 tatataaaat aaagagtaca attaaaaata tacaagctgc tattaagaaa agtagtaaca
    21181 tactgtagcc atacatcaag ccacctaaca atgccccaat agctaccgat aagtttgtca
    21241 tccaatagct aatcttgtaa atataatgtt ccacgtcttc ggtaattgca tccataatta
    21301 atgtgtccat aactggaaat tgtaatcccc aaacgattgt aaatatggca tatgcaacac
    21361 aaaaaccaat aatttgccac aattgatgtg acccaaatac gcccatgaac acaagcatta
    21421 tcaccatcgt cgcttgataa ataagtacta gcaacttttt cggaaatatc tcaataaggt
    21481 aaccagatat aatggacaat ggaaatttta gaaccactaa accaacaaga tatataccga
    21541 caattgattg acttaacata tctgttaaat atagtgctat aaacggtata aatgctgtcg
    21601 taataattag ctgtaaaaaa ttgctaatca atcgtacttt caaagtcgta cttaagcgtt
    21661 tcattaataa ctacacccct ttccacatat atacacacta ccatttatat aaaatctatt
    21721 attagattat acgttgaaac aaagaatttt tagttataca aagttattta acaaacaaat
    21781 gcatcgactt tacaatgcat cttgcatata atgcctcaaa aaaattagca ctatatgact
    21841 atatattatt ccaaatgaat gaaatttatc aataattgcg taagtgaaca catatgtgcg
    21901 taaatgttaa atcattgtcc taacatagct gtgactatac ttagatgtaa tcataaaaaa
    21961 actactgcca tctttacaac agcagtagtg cttatatgat tcatttatat aaaattgaaa
    22021 gcgttaatcg ctagcccagc atccttattc tatttagctg tataactttt actagcaata
    22081 cgaccacttg caacaataat atctttatct tggaaaggtt catgattgaa gaaacgctct
    22141 aaaatatctt taactccatc ttcaatacgt ttttgtgctt ctaaagtcat acctgaatag
    22201 tgaaccgtca tagcatttct aggcattgtt ctccatggat gatcagcagg tgcaggttgt
    22261 ggataccaaa catcaccagc atatccttgt aaatgctcgg atgctaacgc ttcaactaac
    22321 gcatcgcgat ttacaatttt accacgtgca gtattcacta aataactgtg ttttttcata
    22381 cgacttaaaa catctttatc aaataagtta tcagtttctg gtgttaatgg tgcatgaatt
    22441 gtaatcgcat cacttgttga aacaagttca tcaaagctta caaatttaga caatttatgg
    22501 tcttgttgat tgattggatc atagtgttgt aatgttacat taaatggcgc taatctttca
    22561 gcaacaagtt gtccaattcg accaaatcca aaaataccaa ttgttttgtg ttgtaattca
    22621 tgcgcatgat tacctacttg agacaagttc cattcacctt ctactgattg acgatgacct
    22681 tcttcatagt ttctaagaag tattaataaa tccataaccg catgttctgc cacactaact
    22741 gtattacttc ctgtaacttc aacgacacca atattgtgtt cacttgctgc cgctaaatct
    22801 acatggtcag atcctacacc tgctgtaatt gctaatttca agttcggtgc tttttcaata
    22861 cgttcacgag tcatatatgc aggataaaat ggcgcactaa taatcacatc catatctggt
    22921 aaatgtttat ctaagtcttc accattatct gctaatataa tgaactcatg tcctctttcc
    22981 tctaaaaatg tttttaatcc taatgctttt ttagtattaa gtaattgatt ttcttgacct
    23041 tctactgctt ctggaaataa tgctacgatt ttcatatcca tctctcctaa atgataaatt
    23101 ttgacttcct taaagtacgt gttgcatgtt gcttgtttta aaaatatgac aaaaaaacgg
    23161 caccgtttga cataccaccc ccttgaatta ttgttaaatt caaagcgaag ttagcaaaca
    23221 gcaccgtgta ccttacacca gcaacactta ttctattgac ttatcgaaaa caaatattga
    23281 aatgacttca tcatcattca aatcaatatc tgtaaataat tttacaaact tagcgcctac
    23341 aagactttcc atctcattta catctatttc tttatacatc tgtttaatct tctctgtgcg
    23401 tgtataatgg agcatcgatt cattgcgttt gtcgtttagg taaaaactct caactttact
    23461 caaaacacct gtcatatgtg caatcgccca attatcttta aacgacactc gaatactatt
    23521 gggtccttta cccacgtatt ctttacgata agatcttact agattcgaga attgttgttc
    23581 tttagtatgc gtatctatag tattcacctt ctttaaaata agaagttcat taattgttca
    23641 cattttcatt atacacctta ttaacacttt ttcaatgata acgattatta ttattacgaa
    23701 atgtatattt tcctagttaa aaggatataa caaattaaca gaaaatgttt ataaactaag
    23761 atttatatta ttttaaaaaa tgtttcaaca aacgcatcta aacaaaaaat cagcttatca
    23821 cacttacccc ttcaactctg cttcaagttt acctaaatgt cttaatgtcg gtgtcaatgc
    23881 tgcgaagaag aatccttctt ttaacttgcg aacttgtggc gaatatggtg tgtacgctct
    23941 agaaccacca ttgacaacag aagcttgatt tacatctaac aataaatagc cgatgtcctt
    24001 cttcaatgat attaattcat ttaaatgtga agttaagtta ccgtcatcta atattgcata
    24061 atattcctct ctaagttgac gataacgttt tttaaaagct tcaacatcat actctaaata
    24121 ttgatttatt ccgttttgca catttgaaaa tgcatcaatt aactctaaag aacttttaat
    24181 tgagcctaat cctattggaa tttggtaagc aataaattgc gggcgaatag ttgccgcaaa
    24241 ctgcttcgca tcatgcgtga taatttgtga ttgtggcact acgacttgat tcaatgtgat
    24301 ttgatacgtt gctgacccgt tgactcctaa aaaattggtt ttttcaacaa gagtgatacc
    24361 atcttgattg gcacgtagaa tgaacatgac aaattcatct gatgattcat gtttcgaaat
    24421 cgcaccaaaa taatggtctt cttgaatatt acttacagct ggcatacgtc cactgacaac
    24481 caattgtcca tcaacataag tgtgttcaag gttcaacttt tctaaatcat taaatgactt
    24541 catcggatta gacaatccgg tagcacctaa tatttctcca gataacaatt gctgttgtaa
    24601 gtcattattt aaatgtggct gcgtggcatt ttctaaatac gttgaaaaag ctaattggca
    24661 ccataaacaa aatcctgttg tcaaacaaga ctgcgataca gcttcgatta cttcagcatt
    24721 ttttctcaat gccgcctcac cgaaataacc atctacaaat aattgctgaa taaatgttct
    24781 cggataatac gtcccttcat caacttctac taaatgagga tctaattgtt ctttgataag
    24841 cgtttctaaa gtcatcatca tctctcctta ttcaccaaat cttctaagta taaactgctc
    24901 aatataacta atgaatcgat caataataaa accaaataat ccgataaaga atattgctgc
    24961 taaaacatct tctaagttca acatatttcg tgcatcaacg attaaaaaac ctaatcccga
    25021 ttgtgcacca atcatttcac cagaaactaa aaatatccaa cttgttccta ccgccatatg
    25081 tatcccagcc atgatttgtt taaatgcccc gggaaataat atattgcgat acaatgacca
    25141 cccagttaaa tttaaatttg ctgctatttt taaatattga ggttcaatgt ctctaacgcc
    25201 tttaatagta ttgaacacaa ttgggaaaaa agcagcgata aaaataatcg caatcgctgg
    25261 caaactacca ataccaaacc atagaacaac aaatggtgcc catgctatcg gagatatcgg
    25321 cctaatcaat tgaaatagcg gttcgatagc gttgtatagc caacgattcc ttccaagcaa
    25381 gaagcccaat ggaatagcaa ccaacaatgc gacaacaaag cccgctacaa atctccataa
    25441 actaattgct aaatgttgga aaatttctcc agtaacaatg aaagaccata tactttttcc
    25501 tacaagagca ggacccggta acaatacagg ttggtaatgc ccaataataa tgaccatttc
    25561 ccaaatgcct aagaaaataa taaatgtgat aataggtaat ataaatttgt tatttgtggg
    25621 acgtgtcatg aacgcgatgc ctccttatac aatgacggtt caacaaagtc atcatatgca
    25681 ggtggattaa acaaatgatg ttgttttacc aatgtcgtaa tttcttgata gccggatggc
    25741 ttaattgtta aatcaccata ggatgtccat gccgctgact gtgttaaaac gtcacgactt
    25801 tgtttaaaat gatgcgtcat aatgtctaca ctttgcttgc gatcattcat tttaaagcca
    25861 gactttttat aatcttgtac aaatgcttgc gctacatcct tgtgttgatc aagcaattcc
    25921 cctctcagta ctagcacaca gcaatacgca tcaggtataa cgtcatcacc atgtttcaaa
    25981 gtcttacctt tgcctaactt ttcacccagt gcaccgaatg gttcggctac agaataccct
    26041 gtaattctgt gttcactcaa tgcggctggc atttctgctg gcgacatttc atgatagcta
    26101 aaatgccccg gtttaatctt taattgttta cgtaattcct caagtaaaag ataatgtgtt
    26161 gaataacgat gtggtatacc aaaatggtaa tcatcgccat tattattaaa ttcatttaag
    26221 tgcatacctt tttgtcccat aatgacattg ccttcatgat ggcccaatgc cacagccttt
    26281 atatttgagc ccttctgttt tgatttcatc gctagctcta ttaaagttga tgcaccatca
    26341 atacgaccac tgtttaatgc gtccattaaa tctggccaat tattgaattt aactaattct
    26401 agtttatatt tcggatgatt gtattgtgat aataattttt tagtcatcat caaattagct
    26461 gaatgtgtaa tcggcaaata tccaatttta atcacttgct gattttgggc atttttagac
    26521 cgttctttag acgtcctttg ccaatcacat cctgtaatta taaagattcc aatgatgacg
    26581 attatgctta accttttcat cgtcactcac tccttataaa taatattcag gttcaacttg
    26641 atgatgattc aatgcaaatg tttccataat ttcattacga atcttaagta ggtggctatc
    26701 attacgactg cgtggatgtg atgctgtaat ttcatattga gaaataatat tgcacccttc
    26761 acctaacaga acaatgcggt cggaaagata aatagcttca tcaatgtcat gcgtcactaa
    26821 aataatagtt gattgcgttt tatgttttag ttgcactagt tgatcctgaa gtttataacg
    26881 tgtaaatgca tctaatgcac ctaatggctc atccatcgat ataacgttag gcttatgcac
    26941 atgcgctcga catagtgcca cacgttgttt catacccccg gacagttgct cgggaaaatg
    27001 ctttcccctg tcttctaaat caactaattt aagctgtgcg ttaatctctt catcactaat
    27061 tttctgttgt aatccaatcc taatgttgtc attaatcgtt ttccatggca gcaaattatg
    27121 atgttgaaat agcattaaac aatctggaga tggctgttgt ttaatttcgt tatcaataat
    27181 gacacgacca gacgatggat gaataaatcc accgataata ttgagtaaag tagactttcc
    27241 gcaaccactt ttccctatga aagtgactat ttctcccttg ctaatgtcca aattaaagtt
    27301 atgaattact ttatgtgatc caaagtgatg ttgtaattgt tgaattttaa tcatgacaag
    27361 ccccctttta acccctacta ttttaatagg aattaagtgt atatctaaag ggctatgcca
    27421 aacacagccc ttatatcgtg aattgttata tctatagatg attatggttt ggtatgagtg
    27481 ctcttataac ataagcgtta ttcaattaac gacgttatat taatttcagc tctactaaat
    27541 ttcaataaaa tgattgtttt agacaatact tgcatttagt tcattagttt cttcatatca
    27601 actacttaca tttatagcat tgagctattc taattaactg caatcgaaat ccatgccaaa
    27661 cctatataca tatatctcat caacttgttg tcttattaaa gtttttcgat gccatcaatc
    27721 ggtgtatcaa ctgctttgcg cgcgcgacgt accgcttctt catcaggtgc gttgtataaa
    27781 caaatacatt tagacatatc ttcacataca tatgtgcgtt taaattcaac ttctggcact
    27841 tcttcataat gaacagaatt tttcttttta cgtgctaaat attgatccat cgtaatgcct
    27901 tccggaatgt tccaagttac aaggtaatca acatgtgcat caccattttt aacttcatct
    27961 aaatctttac caactaagcg tacttctttg actaattgaa tactaatatc tgcttctgtt
    28021 aaaaattgtt tagcttctgc ttcgtcattt gcttccacaa taaaataacc atgagttaaa
    28081 ttttcagtag cttgtacttc aattaatgtt ggaattgtct tcgtagatag tgatgctgcc
    28141 tttgcttcta gttcttcttt cgttgatgca aaatcaagat tgttagcttc taataaaaat
    28201 aatgtcatta ataagacctc ctataacaat ggttgaaaaa atattactat aattcttatc
    28261 ggattacaag gatattaaga tattttttta aatatttaga taaaattctg taaaatatat
    28321 tttcaaagag tataaaaatc atgttagggt ataagtaaat aaacatatat atagggggct
    28381 atcattatgg ataaagaatc tgtcgtagca agtttagcta gaaacaaaaa gattgctgta
    28441 gaaacaatgg ccggtcaaag gtacatcata gaacgcattt tacatacaaa tgatgaaaaa
    28501 catattcata ttttgaaacc taaagatgtc gtgttagatg tagatagcat caaagaaatt
    28561 gacgaaaatc atttaaatga tgcaacataa gtataaaacg aaacgtgcgc ctatgacata
    28621 gattaatgtt ttaggcgcta caatgtttta tttatgttaa tttgtcgaag tatggctcat
    28681 ctaatggatt caattgcaag ttttcaattt gcaacgttga atgtgcaatg ccatattttt
    28741 ctttcaacaa tgatgatact tgatcaatcg cttgataatc atcaccctca tattttttat
    28801 ctaacacaac atgggcactt aatgaataat gctctgttgt aatactccac aaatgaaatt
    28861 catgtacatc taatatgcca tctatgtttt taatatctgc cataatttga tcagtatcca
    28921 aatgttgagg cacactttcc attaaaatta accacgcatt acgcgtaatt ttataaccac
    28981 cacgtaaaat gatgagtgaa attacaatac taatgattgg gtcgatgatg cgccatcctg
    29041 taaagtaaat caatacaact gcaacgatga caccaataga gttcaataag tctcccatga
    29101 aatgccataa tgcactttga atattgatat tgtcttcttg ttttaaagac cttacaagga
    29161 taacagtcaa aataatattg acgagtaaac caatactagc aatcataaac ataatgccac
    29221 tttcaattgg ttgcggataa ataatacgta caatagcttc atataaaatc cagattgaaa
    29281 ttacaattaa tgctaaacca tttaaaaatg cagctaatat ctcaaatctt aaatatccaa
    29341 atgtgtatcg tgcagtcggc tttttacttg caaaataaat ggccaacata gataaaccaa
    29401 gtgctaatac atcactaagc atatgaaatg aatctgacag taatgccaat gaattagata
    29461 ctaaacctcc gacaaattca atcactgtaa ataacaatgt gatgattagt gatgcccaca
    29521 gcgtcttttt agaactttgt tgaatttttt gatgattgac atgatgaaaa tatttgtatc
    29581 tgtttatcgt attcgccttt ttcccattca tacgtttgcc actcctaaat aaatgcgtcg
    29641 tatttaatat tgtcgctttt aaaaataatt ctttatagct tctcaggtta atattaatgc
    29701 ttccgtaatt aaccatgatt tatacatcat cgtcatttac atgacaactc attaatcttt
    29761 accctttata gctttatgta atatcacctt ggaaaacttt tacagcacac tcgcgctttg
    29821 ctttattcct atcatataga aaattttaat aatgtacgag attattgtgt tagttacata
    29881 aaaatgacaa aaaagtatct caaatttatt gtttagtttc attaagttca aaaaatcgca
    29941 ataactttaa tgattgttcc gcatattaaa acaaacgatg actttgcata ttgaacttca
    30001 tatttctaaa tcaccataat taataaacaa ctctgtcctt ataatagccg catcgcaaat
    30061 tagcataatt aaaaacagca gtaagatgat ttgcaattaa aaaccatcct actgctatta
    30121 ttttagtcat tatatcctaa tatcatgata cgtgttccta tatattttgt tttgcttaaa
    30181 atctaaacaa cgccgttaaa tgttaagact taccgctaga ctgacctaca aacacgcttc
    30241 agaaacgata tttattctag tacaaacctt ttaaagcatt gcttgtatca atataaatat
    30301 ttttaacttg ttgatagtta cttaacgcac ctttataagt ttctcgaccg atacctgatt
    30361 ttttataacc accaaatggt gcgccttctg gtacttggtt gtaagtgtta atccaaatac
    30421 gtcctgtacg tacagcttta gcaatattta atgcacgtgt gatattttga gaaaatacac
    30481 cgcctgctaa accatactca gaatcattag ctatatcaat tgcttcttga tcgtccttca
    30541 ctttaatcac tgttaacact ggtccaaata tttcttcttg tgctaatttg tgatgattgt
    30601 ctggcacagc aattaatgtc ggctcaaaga agaacccttt atctaatcca ttttcagtta
    30661 agcgatgacc gcctgctaaa atttgtgcat ctgattcttt tgctgcatca atatatgatt
    30721 gaattttatc taattgatcc ttaccagttt gactacccat ttgtgtagct tcatcttgtg
    30781 gatttccaac tttaatattt gaaaatgcct cttgtaaacg tggcaccaat tgatcataaa
    30841 ttttttcatg aactaataat cgagaacctg cactacatac ttcaccttgg ttgaataaaa
    30901 tacctaactg aataccttca actgcaaggt ctaaattagc atcatctaat atgatattgg
    30961 cgcttttacc accaagctct aatgtagcgg gtactagatg ttttgctgca gcttcggcaa
    31021 cttgataacc tacatcagtt gagcccgtaa atgataattt atctacacca tcatgattga
    31081 aaattgcatt acctgattct gaacctttac ccgttagtat attgacaaca cctttaggta
    31141 atacctcttg gaaaatttta gcaacttcca ataaacttaa tggtgttgaa gacgaaggtt
    31201 gaatcacaat tgtattacct gcagcaatgg ctggcgcaat cttccatgca gctaatagca
    31261 ttgggaagtt ccaagcaaca acagcaccta cgacgccaat cggctcatgt cgtacgatac
    31321 tcattgtgtc tttatcgata tcattcactg taccttcttc tgtttcaata acacttgcga
    31381 aataatggaa atgtcttgca gcaaatggaa tatcaattgc tgttgtctca cgaatcggtt
    31441 taccattatt taatgtttca atcattgcaa ttttatcttt ttgtgccatt aatttatcac
    31501 caatatcacg caacatttgt gcacgttctg atttagaagt taatgaccat gattcaaatg
    31561 cctcttgcgc cactttgact gcatgatcga catctttatc ttttgctctt gtaatatgtg
    31621 atagtgtttc tccagttgct ggattagtca cttcgattgt ttcgtcactg ctacctttaa
    31681 caaattcccc attgataaat aaaccataat tctctgcaat ataatctcga acgtttactg
    31741 ccatattatc tctcctttgt ttattggcta taatataaat ctcttagcat catatttaat
    31801 aatgctttta aacgatatga ttcacattat atagtaagcg tttacatttt ataaaataat
    31861 cagctcacag aagtctcatt tttaaaactt ttgcgtcgtt tttgcacata aaaataatct
    31921 tattttcgaa aacatattgc acttttcaaa aataaaacac ataatataac tataatactt
    31981 gagaataatt ctcattaaat agtttggttg ttatgaaagt cgcgcttaaa atgcgttaat
    32041 gtgacaagga taattcaaca tcgtttcatt ttactgagtc attgcactta tcatacacat
    32101 tatatttagc atgagttata ttactaaaac aatattacat gcgcattagt actagaatgg
    32161 agttttttca atgcgactta ttttaatcgt cattggctta atatttacag cgcttggtat
    32221 tgcaggtgcc gtattacctt tactgccaac gacccctttt ttactcgtag cagttttttg
    32281 ttttgctcga agttcagatc gcttttacaa ttggcttatt aatcaaaaaa tttataaaga
    32341 atatgtagaa aacttttatt tacatcgagg ctacacgcta caacagaaaa ttaaaatttt
    32401 aattagctta tacattgtaa taggtttttc aatttatatg gtggatgttc ttgcagtccg
    32461 tgtaggatta atcataatgg ttatcataca aaccgctgta ctctttacat ttgtaaaaac
    32521 attacccaaa tcaaatcata aaatagagga gtgattgccc atgtttatgg cagaaaatag
    32581 attacaatta caaaaaggca gtgcggaaga aacgattgaa cgtttttaca atagacaagg
    32641 tattgaaact attgaaggct tccaacaaat gtttgtcact aaaacattaa ataccgagga
    32701 tacagacgaa gttaaaatct taactatttg ggaatctgaa gatagcttta ataattggtt
    32761 gaattccgat gtatttaaag aagctcataa aaatgtacgt ttaaaaagtg atgacgatgg
    32821 acagcaaagt ccaatattat caaataaagt attcaaatat gatattggct accactatca
    32881 aaaataaata tattaaaaac ccctagtcac cgctggttgt ctaaaccaac ttcatgatta
    32941 ggggtttttt attatttgtg acgtaaaggt acgaattcat ccggcttgtc tgtgcgcaat
    33001 ccaaaataat attctattgc ttcacaaata cgtcgtgatg cttgtccatc accatatgga
    33061 ttattcgctt gactcatacg ttgatacgct tcatcatcct caatcaattg tttcacattt
    33121 cgaacaatat tgtcataatc tgtgccaatt actctcgatg ttcccgcctc aacgccttcg
    33181 ggacgctctg tatgattcct taataccaac acaggttttc caaatgtagg agcctcctct
    33241 tgaataccac cagaatctgt cagcacgagg tacgattgat ttgtaaaatt atggaactca
    33301 atcgcatcta atggctcaat taattcaatc cgatttctcc cagataaata tttttcggca
    33361 atcgctctta cctttggatt acgatgcatt ggataaatga agacaacatc tttatattca
    33421 tctgccaaat ctcttactgc tttaaaaatc tgatgcatcg gttccccaat attttcacga
    33481 cgatgcgctg ttagtaaaac aactttcttg cctttatgtt tattaataat cgttgaaaca
    33541 aaatcatttt gaactgttgt tgacaatgcg tcaataactg tatttccagt aataaagata
    33601 cgctctttgt ctttgttttc aaaaagtaaa tttttagctg caattactgt tggcgcaaaa
    33661 ttcaattcag caatattact taccatgact cgatttaact cttcaggaaa tggtgagtat
    33721 ttctgatgtg ttcgaagtcc agcttctaca tgtccgaccg gaatttgatg ataaaatgct
    33781 gccaaacttc ctacaaaagt cgttgtagta tcaccatgta ctaaaatcat atccggttgt
    33841 tcctcattaa tgatgctatc aagtttagca agtgcattcg ccgtaaggcc tgctaatgtt
    33901 tgttgatctt gcataatatt taaatcatga tcagcttgaa tatcaaatat acttaacaca
    33961 ctatctaaca tatctctatg ttgtgctgta atcacaatgt tcgcttcaaa gttcccatta
    34021 tgatcaattt cttttactaa tggtgccatt tttattgctt cgggtctcgt accgaaaata
    34081 accataattt ttttcataac attatcctct ctgaagttca aacacatgac agcttatctt
    34141 tccttaatca tgtatgccct agtttgacac attttatttg tcgataaaat taaatatatt
    34201 gccataatta taatacgata catcttcaaa tgatgatttc acaacatttt ttgtatcaaa
    34261 aatcacttta tgcttcattt tatcaaaatg actgtccgat aaatttttaa attctgagtg
    34321 gtcacttaaa atcaatacta gcgatgcgtc tttgacagca tgtgacatat catgttccac
    34381 aaaatctaat tcaacatgtg gatcataagc acatacttct atgtctggtt cttgatttaa
    34441 tagctcataa atatcaaatg ctggtgattc tcttatatca tcaacatcac ctttataagt
    34501 taaaccaaat actgtgactt tattcccgct caacactttg atgatttgct tcgttgtatc
    34561 aacaacatag gccggcattg aattattaat ttcacgtcca gtttgaatta actttgcatt
    34621 ttcagggtct ttagcaataa taaagtacgg atcaacagct aaacaatgac cgcctacacc
    34681 tggaccaggc tgatggatgt taacacgcgg atgtttgttt gccatttcaa tcacatctaa
    34741 tacattaata tttaagttat tgcaaatttt tgttaattca ttagctaaag caatgttcac
    34801 gtctctatat gtgttttcca ttagcttact catttcagca gtacgtgcat ctgtttcaat
    34861 catttctccc tgaacgaatg tgcgatagac acgtttaccc gcttcaatac aagcttcagt
    34921 cacaccgcca atgatacgat tgttatgaac taattcttct aaaatttttc ctggcagtac
    34981 acgttctgga caatgcacta aataaatatc ttcacctatt gtaaacccta aattttcaat
    35041 gactggtttt acaaaatcat ccatcgtttt aggcgcaatt gtcgactcta caataatggt
    35101 atttcctttt tctaaaaatg ataaaatact atctaatgca cgcataacta gcgaaatgtc
    35161 acatgaccgg tactgatcat cattattcgg cgtcggaacg gcaatgataa aaacatcaga
    35221 tgcatctggc gttgtagata ccttcaattt tcccgatgac agtacctctt cataaacctc
    35281 ttgtaatcca ggttcttcaa tactaatttg accactttgt aacttatcaa tcgtttgctg
    35341 attaatatca acaccaagca catcgacgcc atgttttgca aacataattg atgttggtaa
    35401 accaatataa cctaagccaa ctactgttaa cttcatacta taacctccaa ataaaacgat
    35461 ttgtatgttg attttattta atagatgact tatgccttat ctttggttgt cgtttcatcc
    35521 atgatgtccg caataacaag tgacattttt ccaggaataa tttcaagtgc attattattt
    35581 tcatataacg tattgctgta tattaaattg ccgaataatt ttctaaagac cgacaactta
    35641 ttaaaatact tatttagcat tgaaggcatg ttgatcaata ccgttttacg atgtgattgg
    35701 cgacgtattt catacattac tgacgatgta tcaaagtaaa aactatcttg aggatggtac
    35761 acacctgtca cttctaatga tattaattga tcaataaatg ctgtcagatg tttaatatat
    35821 aatgcactgc gctgattgtt aatattggga atgattggca atcgctttga caattgcatt
    35881 aaccgttgga aatttcctgg gcaatgtgca ccataaatca ttggtggtct cacaattgct
    35941 actttaaacg aatcactaat caattcttgt aatgcttgtt cagcgaactt tttggaaata
    36001 ccatagttgg tcgtagggtt cattggtgtt tgtgtatcaa cttgatctga tttaccaaca
    36061 tgaccttctt ttccataaac tgccatagta ctcataaaaa taaattgttt aacgtcttca
    36121 gctttagcct tttgtgccaa ttgtttcgtc agcaacatat tcacttgcat ataatcagat
    36181 agccttgctt gaggtgaatt gttgtgaacc aaagctgctg tatgaattaa aacatcataa
    36241 tctttgaacg aggtcgactt ccataattga ttcctaacat taatttgatc tacttgatgt
    36301 ccttgttcaa taagcttatc ttttaaagca ttaccgatat atccatgtac gcctgtaatt
    36361 aaaatatttt ttctcattag tgatgcacac cttctgaagt aacgatattt ttaattgttt
    36421 tatatatgat atacatatca agcatcatag attgatgtgt taagtaataa tgatcatacg
    36481 ctactttttg atcatcagtg atatcatctc tccccatcac ttgagctagt cctgtcacac
    36541 ctggtctaat cgtatgcacg ttcgcttttg tacgtttttc gattaattcg tattgattat
    36601 aaagcgctgg tctaggacct acaattgaca tttctccttt taaaacattc aataattgtg
    36661 gcaattcatc aatagaggtc ttacgaatga ccttccctgt ctttgttata tacgatgttg
    36721 aatccattaa atcagttgca acattaggtg tgtctatttt cattgatcta aacttataaa
    36781 tattaaacaa ttcattatta atcgtcggtc ttttttgttt gaaaatggct ggtccaggtg
    36841 attccatttt aattagtaat gctgtaatta acagaatcgg acttaaaact actaaaccat
    36901 atattgaact cactacatcg aataatcgct tcataattat ctcctcactt caacagattg
    36961 taatacttca tagtattaat ttctctcgta tatttatctt taaacagttg atgtgcgcgc
    37021 tcacccatct cgtgcaattc cttactcgag tgagtgttga tgaagttata aatggcatgt
    37081 gcatcgccat tatcaatttg gatacccgca tcatattgtt caacatgttg aacgatatca
    37141 gattgcttat ccatgatgag taccaacgct ttcttagctg caagatagcc ataattcttg
    37201 ctcggcacgc ctaaaccgac gccttcttta atcagcgatg caatacatac atccgcaatt
    37261 tttaatacgt cagcatagtc tgtacctgtt aaaaactcaa acattttaac attttcaata
    37321 cgatggtctt ctattgccgt tttgacatct gcaaatttct taccatgacc acaaagtatt
    37381 gttaacgttt gagactgatc cttatttaat tttaaaaatg agataagtgt ctccatatcc
    37441 tgtaactgcc ccatattacc gctatacaat aaaattttgt cgtattgctc acggtaagct
    37501 ttaaatgtgt cattatagat acgattgtct tgtaattgac gcatgtcata ccagttagga
    37561 atcacatgga tattgtcagc atttttagaa atttgatgat ttagtaagta gtttttcatt
    37621 tccgtaccaa ggacaatgac attttcagca ttcttgtaga catgtctatt aatgtaacgc
    37681 atcagcttat caatcatgct acctggacga gttgcacctg tcttaatcgc attatcaggt
    37741 gctatatcat acaccacaaa agaatatttt ttcttaagca gtctgtgtaa aacgtctggt
    37801 attaatggca agattggtgg attagagtaa acaagaatct gatcatattt caacatttta
    37861 ggtatattaa tcacgaattt tgaaaataaa ctaaagaaat tgatgatcct tccaacctta
    37921 cttttgttat taaacctcga atacttgaga cgtcgaatgc gaataccacg atgcatctcg
    37981 gttttagaaa cctgtttatg attactatat tcatatggcc atccacacat gacatcgaca
    38041 ttaatgtgat tcgcaattaa atcttccgcc aattgagttg gtaacgtcgc agaagataca
    38101 tattccggat aaaaatactg acataaaatc aaaatctttt tttcactcat ctaatcgacg
    38161 tcctttttat taatgaattt agactttaaa cctataatat ttaaaatcac atacttaata
    38221 atgttgctga acagcgttgt ccaaaagaat ccatttagac caaacgcaat tgtcattaaa
    38281 atagttatga atataaatgt aatcgtgtga agcgtcatat aattcgcttg taatgttatt
    38341 gatgcgtgtt ttgtatttaa agtttggatc ataatactca ctgcattgaa taaaacacct
    38401 atattaccta aaataataaa cttcgaatat aaacttgaat caatgttata cagtaaagag
    38461 acaataatta ttgtaattgg ataacatata atcatgacta aacatagggc agctatagca
    38521 attagattag ttttcaaata ttgcttcttt atattgtcac tttcatttac agaaatatat
    38581 gaaagtacta cattattaat cggatataga aatgtagcta acattttccc aataaatgtt
    38641 gaaagaaatg atatagttac agctgttcca cctataattg gtaataagat taatctatct
    38701 aaatagagat tcaaattatt aaggctattt gtactcagta gcatcacata atcttttacc
    38761 acattattat cttcactttg atactcgcct atagttaatc cccgtaattt aaccaatgta
    38821 tatatcgttg caaacaattc actggtaata aaacaaacaa tccagttttg ggttaaataa
    38881 tatagaaata gtcctatcag caaacctaaa aattgaataa gagcaatata caaaatctga
    38941 ttatatttta aagtcatcct aaaaaataca ttcagataaa tccttaaaca cattaaaata
    39001 ttaagtagaa ttaaaaagat aatatcgatg atgttcaaat
//
LOCUS       DAKTIO010000006        37847 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.006, whole genome shotgun sequence.
ACCESSION   DAKTIO010000006 DAKTIO010000000
VERSION     DAKTIO010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 37847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37847
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.006"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            54..1007
                     /locus_tag="O0293_000207"
     CDS             54..1007
                     /locus_tag="O0293_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279120.1"
                     /translation="MKKTLGCLLLIMLLVVAGCSFGGNHKLSSKKSEESKQETVKKES
                     EEEKDPDLEKYEEIEKKMKGIKDAPSLDKLDPLMTEKSFTNSKGIQGWKDYKELMGKV
                     ELADYRFTKDSKGSSIKDVDAFFKGKKGIKRKVIETHDDVKQVDYWYVDPDGKKIGNS
                     NTPVFYAEIMTKYKDGKLVYASVEPGSYVIHKDDAIKYDDYSKLKKLSQLTKLDHPKP
                     VPYSVAQIKSFGVPLTSVSFMTHGSKDTKDEVLPALAYFTFSPKNYEDKSNPDPKVLN
                     LVHMDFLNASSDFGNAHFVVLSKYIKEYESNYETASDDSLK"
     gene            complement(1170..1373)
                     /locus_tag="O0293_000208"
     CDS             complement(1170..1373)
                     /locus_tag="O0293_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HCY0279121.1"
                     /translation="MQVNKVIYILLALFLGSFGIHKFYAGKNMQGILHLIFCWTGIPH
                     ILAIISAVITVFKPADEQGNVTL"
     gene            1672..2160
                     /locus_tag="O0293_000209"
     CDS             1672..2160
                     /locus_tag="O0293_000209"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCY0279122.1"
                     /translation="MAIKKLVPASHPILTKKAQAVKTFDDSLKRLLQDLEDTMYAQEA
                     AGLCAPQINQSLQVAIIDMEMEGLLQLVNPKIISQSNETITDLEGSITLPDVYGEVTR
                     SKMIVVESYDVNGNKVELTAHEDVARMILHIIDQMNGIPFTERADRILTDKEVEAYFI
                     ND"
     gene            2153..3088
                     /locus_tag="O0293_000210"
     CDS             2153..3088
                     /locus_tag="O0293_000210"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCY0279123.1"
                     /translation="MTKIIFMGTPDFSTTVLEMLIAEHDVIAVVTQPDRPVGRKRVMT
                     PPPVKKVAMKYDLPVYQPEKLSGSEELEQLLQLDVDLIVTAAFGQLLPESLLALPNLG
                     AINVHASLLPKYRGGAPIHQAIIDGEQETGITIMYMVKKLDAGNIISQQAIKIEENDN
                     VGTMHDKLSVLGADLLKETLPSIIEGTNESVPQDDTQATFASNIRREDERISWNKPGR
                     QVFNQIRGLSPWPVAYTTMDDTNLKIYDAELVETNKINEPGTIIETTKKAIIVATNDN
                     EAVAIKDMQLAGKKRMLAANYLSGAQNTLVGKKLI"
     gene            3085..4392
                     /gene="rsmB"
                     /locus_tag="O0293_000211"
     CDS             3085..4392
                     /gene="rsmB"
                     /locus_tag="O0293_000211"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCY0279124.1"
                     /translation="MIENVRSLAFDTIQDILNEGAYSNLRINEVLSENELNAMDKALF
                     TEIVYGTVKRKYTLDFYLKPFVKTKIKAWVRQLLWMSIYQYVYLDKVPNHAIINEAVE
                     IAKERGGYHNGNVVNGILRTMMRSDLPDFNEIADPKKRMAIEYSMPKWIIDHWATHYG
                     LEETETILQSFLETTSTTVRANLTRASLDDIIEKLQDEGYDVEKDHDLPYCLHIGGQP
                     IIHSRSFKDGFVSIQDKSSMFVAHIMNVDRHDHVLDACSAPGGKACHIAEVLMPEGQV
                     DASDIHDHKIDLINFNIKKLRLTNIKAFQHDATKPYDKTYDKILVDAPCSGLGVMRHK
                     PEIKYTQSKQHIESLVELQLEILENVKNNVKIGGEIIYSTCTIEQLENENVIYTFLKN
                     NKNFEFEPFQHPITGELVKTLQIMPQDFNSDGFFITKIKRKDN"
     gene            4395..5489
                     /gene="rlmN"
                     /locus_tag="O0293_000212"
     CDS             4395..5489
                     /gene="rlmN"
                     /locus_tag="O0293_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002435140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCY0279125.1"
                     /translation="MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAK
                     QIFEWLYQKRVDSIDEMTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLFELQD
                     GYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKA
                     LDATEERVSQIVIMGIGEPFENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIY
                     DFADEDIQINFAVSLHAAKDEVRSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYG
                     LFGGVNDQLEHARELAHLIKGLNCHVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLG
                     INATIRREQGSDIDAACGQLRAKERQVETR"
     gene            5496..6239
                     /locus_tag="O0293_000213"
     CDS             5496..6239
                     /locus_tag="O0293_000213"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase Stp1"
                     /protein_id="HCY0279126.1"
                     /translation="MLEAQFFTDTGQHRDKNEDAGGIFYNQTNQQLLVLCDGMGGHKA
                     GEVASKFVTDELKSRFEAENLIEQHQAENWLRNNIKDINFQLYHYAQENAEYKGMGTT
                     CVCALVFEKSVVIANVGDSRAYVINSRQIEQITSDHSFVNHLVLTGQITPEEAFTHPQ
                     RNIITKVMGTDKRVSPDLFIKRLNFYDYLLLNSDGLTDYVKDNEIKRLLVKEGTIEDH
                     GDQLMQLALDNHSKDNVTFILAAIEGDKV"
     gene            6236..8230
                     /gene="pknB"
                     /locus_tag="O0293_000214"
     CDS             6236..8230
                     /gene="pknB"
                     /locus_tag="O0293_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase Stk1"
                     /protein_id="HCY0279127.1"
                     /translation="MIGKIINERYKIVDKLGGGGMSTVYLAEDTILNIKVAIKAIFIP
                     PREKEETLKRFEREVHNSSQLSHQNIVSMIDVDEEDDCYYLVMEYIEGPTLSEYIESH
                     GPLSVDTAINFTNQILDGIKHAHDMRIVHRDIKPQNILIDSNKTLKIFDFGIAKALSE
                     TSLTQTNHVLGTVQYFSPEQAKGEATDECTDIYSIGIVLYEMLVGEPPFNGETAVSIA
                     IKHIQDSVPNVTTDVRKDIPQSLSNVILRATEKDKANRYKTIQEMKDDLSSVLHENRA
                     NEDVYELDKMKTIAVPLKKEDLAKHISEHKSNQPKRETTQVPIVNGPAHHQQFQKPEG
                     TVYEPKPKKKSTRKIVLLSLIFSLLMIALVSFVAMAMFGNKYEETPDVIGKSVKEAEQ
                     IFNKNNLKLGKISRSYSDKYPENEIIKTTPNTGERVERGDSVDVVISKGPEKVKMPNV
                     IGLPKEEALQKLKSLGLKDVTIEKVYNNQAPKGYIANQSVTANTEIAIHDSNIKLYES
                     LGIKQVYVEDFEHKSFSKAKKALEEKGFKVESKEEYSDDIDEGDVISQSPKGKSVDEG
                     STISFVVSKGKKSDSSDVKTTTESVDVPYTGKNDKSQKVKVYIKDKDNDGSTEKGSFD
                     ITSDQRIDIPLRIEKGKTASYIVKVDGKTVAEKEVSYDDV"
     gene            8458..9333
                     /gene="rsgA"
                     /locus_tag="O0293_000215"
     CDS             8458..9333
                     /gene="rsgA"
                     /locus_tag="O0293_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HCY0279128.1"
                     /translation="MKTGRIVKSISGVYQVDVNGERFNTKPRGLFRKKKFSPVVGDIV
                     EFEVQNINEGYIHQVFERENELKRPPVSNIDTLVIVMSAVEPNFSTQLLDRFLVIAHS
                     YQLNARILVTKKDKTPIEKQFEINELLKIYENIGYETEFIGNDDDRKKIVEAWPAGLI
                     VLSGQSGVGKSTFLNHYRPELNLETNDISKSLNRGKHTTRHVELFERQNGYIADTPGF
                     SALDFDHIDKDEIKDYFLELNRYGETCKFRNCNHIKEPNCNVKHQLEIGNIAQFRYDH
                     YLQLFNEISNRKVRY"
     gene            9334..9978
                     /locus_tag="O0293_000216"
     CDS             9334..9978
                     /locus_tag="O0293_000216"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCY0279129.1"
                     /translation="MTKLYPSLLSVDFLDLQHELKRLEEAGVDGVHFDVMDGQFVPNI
                     SIGLPILDAVRKGTTLPIDVHLMIENPEKYIASFAEHGADMISIHVESTPHIHRAIQM
                     IKHLDKKAGVVINPGTPISQIEPILDIVDYVLVMTVNPGFGGQSFIDQCVEKIAGLNA
                     IKMERQLNFDIEVDGGVNTDTAKVCVENGATMLVTGSFFFKQEDYKKVTQALKG"
     gene            9985..10626
                     /locus_tag="O0293_000217"
     CDS             9985..10626
                     /locus_tag="O0293_000217"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HCY0279130.1"
                     /translation="MHINLLCSDRHLPQDIWAKSNEGKWGGVDRGALILLKHQIIPFF
                     SVGDFDSVSKEERQLLTEQLQIKPVQAEKADTDLALAVDKAVALGFDSITIYGATGGR
                     LDHFFGAIQLLLKKAYYKHDVHIEVIDQQNKIELLPKGQHTVEKDKSYPYISFIPMTD
                     DVELSLAGFKYNLARQMLNIGSTLTISNEIESLQAKVTVHDGLILQIRSTDLN"
     gene            complement(11007..11195)
                     /locus_tag="O0293_000218"
     CDS             complement(11007..11195)
                     /locus_tag="O0293_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCY0279131.1"
                     /translation="MGKQCFVTGRKASTGNRRSHALNSTKRRWNANLQKVRILVDGKP
                     KKVWVSARALKSGKVTRV"
     gene            11638..12012
                     /locus_tag="O0293_000219"
     CDS             11638..12012
                     /locus_tag="O0293_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCY0279132.1"
                     /translation="MTLEISNDYGKIDISNEVIASVVGGKAVECYGIVGMASRQQVRD
                     GIAEILGHENYAKGIKVTENNGVVDIDMYIIVSYGVKISEVANNVQSTVKYTLEKSLN
                     VSVNSINIYVQGVRVNNTGKKA"
     gene            12027..13673
                     /gene="fakA"
                     /locus_tag="O0293_000220"
     CDS             12027..13673
                     /gene="fakA"
                     /locus_tag="O0293_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase catalytic subunit FakA"
                     /protein_id="HCY0279133.1"
                     /translation="MISKINGKLFADMIIQGAQNLSNNADLVDSLNVYPVPDGDTGTN
                     MNLTMTSGREEVENNLSKNIGELGKTFSKGLLMGARGNSGVILSQLFRGFCKNIESES
                     EINSKLLAESFQAGVETAYKAVMKPVEGTILTVAKDAAQAAIEKANNTEDCIELMEYI
                     IVKANESLENTPNLLAVLKEVGVVDSGGKGLLCVYEGFLKALKGEKVEAKVAKIDKDE
                     FVHDEHDFHGVINTEDIIYGYCTEMMVRFGKNKKAFDEQEFRQDMSQFGDSLLVINDE
                     EIVKVHVHTEYPGKVFNYGQQYGELIKLKVENMREQHREVIRKEQHTAKPKMETVETA
                     IITISMGEGISEIFKSMGATHIISGGQTMNPSTEDIVKVIEQSKCKRAIILPNNKNIL
                     MASEQAASIVDAEAVVIPTKSIPQGISALFQYDVDATLEENKAQMADSVNNVKSGSLT
                     YAVRDTKIDGVEIKKDAFMGLIEDKIVSSQSDQLTTVTELLNEMLAEDSEILTVIIGQ
                     DAEQAVTDNMINWIEEQYPDVEVEVHEGGQPIYQYFFSVE"
     gene            13863..15923
                     /gene="recG"
                     /locus_tag="O0293_000221"
     CDS             13863..15923
                     /gene="recG"
                     /locus_tag="O0293_000221"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCY0279134.1"
                     /translation="MAKVNLIESPYSLLQLKGIGPKKIEVLQQLNIHTVEDLVLYLPT
                     RYEDNTVIDLNQAEDQSNVTIEGQVYTAPVVAFFGRNKSKLTVHLMVNNIAVKCIFFN
                     QPYLKKKIELNQTITVKGKWNRVKQEITGNRVFFNSQGTQTQENADVQLEPVYRIKEG
                     IKQKQIRDQIRQALNDVTIHEWLTDELREKYKLETLDFTLNTLHHPKSKEDLLRARRT
                     YAFTELFLFELRMQWLNRLEKSSDEAIEIDYDIDQVKSFIDRLPFELTEAQKSSVNEI
                     FRDLKAPIRMHRLLQGDVGSGKTVVAAICMYALKTAGYQSALMVPTEILAEQHAESLM
                     ALFGDSMNVALLTGSVKGKKRKILLEQLENGTIDCLIGTHALIQDDVIFHNVGLVITD
                     EQHRFGVNQRQLLREKGAMTNVLFMTATPIPRTLAISVFGEMDVSSIKQLPKGRKPII
                     TTWAKHEQYDKVLMQMTSELKKGRQAYVICPLIESSEHLEDVQNVVALYESLQQYYGV
                     SRVGLLHGKLSADEKDEVMQKFSNHEINVLVSTTVVEVGVNVPNATFMMIYDADRFGL
                     STLHQLRGRVGRSDQQSYCVLIASPKTETGIERMTIMTQTTDGFELSERDLEMRGPGD
                     FFGVKQSGLPDFLVANLVEDYRMLEVARDEAAELIQSGVFFENTYQHLRHFVEENLLH
                     RSFD"
     gene            16126..16698
                     /gene="fapR"
                     /locus_tag="O0293_000222"
     CDS             16126..16698
                     /gene="fapR"
                     /locus_tag="O0293_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HCY0279135.1"
                     /translation="MRGETLKLKKDKRREAIRQQIDSNPFITDHELSDLFQVSIQTIR
                     LDRTYLNIPELRKRIKLVAEKNYDQISSIEEQEFIGDLIQVNPNVKAQSILDITSDSV
                     FHKTGIARGHVLFAQANSLCVALIKQPTVLTHESSIQFIEKVKLNDTVRAEARVVNQT
                     AKHYYVEVKSYVKHTLVFKGNFKMFYDKRG"
     gene            16703..17689
                     /gene="plsX"
                     /locus_tag="O0293_000223"
     CDS             16703..17689
                     /gene="plsX"
                     /locus_tag="O0293_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCY0279136.1"
                     /translation="MVKLAIDMMGGDNAPDIVLEAVQKAVEDFKDLEIILFGDEKKYN
                     LNHERIEFRHCSEKIEMEDEPVRAIKRKKDSSMVKMAEAVKSGEADGCVSAGNTGALM
                     SAGLFIVGRIKGVARPALVVTLPTIDGKGFVFLDVGANADAKPEHLLQYAQLGDIYAQ
                     KIRGIDNPKISLLNIGTEPAKGNSLTKKSYELLNHDHSLNFVGNIEAKTLMDGDTDVV
                     VTDGYTGNMVLKNLEGTAKSIGKMLKDTIMSSTKNKLAGAILKKDLAEFAKKMDYSEY
                     GGSVLLGLEGTVVKAHGSSNAKAFYSAIRQAKIAGEQNIVQTMKETVGESNE"
     gene            17682..18608
                     /gene="fabD"
                     /locus_tag="O0293_000224"
     CDS             17682..18608
                     /gene="fabD"
                     /locus_tag="O0293_000224"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCY0279137.1"
                     /translation="MSKTAIIFPGQGAQKVGMAQDLFNNNDQATEILTSAANTLDFDI
                     LETMFTDEEGKLGETENTQPALLTHSSALLAALKNLNPDFTMGHSLGEYSSLVAADVL
                     SFEDAVKIVRKRGQLMAQAFPTGVGSMAAVLGLDFDKVDEICKSLSSDDKIIEPANIN
                     CPGQIVVSGHKALIDELVEKGKSLGAKRVMPLAVSGPFHSSLMKVIEEDFSSYINQFE
                     WRDAKFPVVQNVNAQGETDKEVIKSNMVKQLYSPVQFINSTEWLIDQGVDHFIEIGPG
                     KVLSGLIKKINRDVKLTSIQTLEDVKGWNEND"
     gene            18601..19335
                     /gene="fabG"
                     /locus_tag="O0293_000225"
     CDS             18601..19335
                     /gene="fabG"
                     /locus_tag="O0293_000225"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HCY0279138.1"
                     /translation="MTKSALVTGASRGIGRSIALQLAEEGYNVAVNYAGSKEKAEAVV
                     EEIKAKGVDSFAIQANVADADEVKAMIKEVVSQFGSLDVLVNNAGITRDNLLMRMKEQ
                     EWDDVIDTNLKGVFNCIQKATPQMLRQRSGAIINLSSVVGAVGNPGQANYVATKAGVI
                     GLTKSAARELASRGITVNAVAPGFIVSDMTDALSDELKEQMLTQIPLARFGQDTDIAN
                     TVAFLASDKAKYITGQTIHVNGGMYM"
     gene            19578..19811
                     /locus_tag="O0293_000226"
     CDS             19578..19811
                     /locus_tag="O0293_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002510960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCY0279139.1"
                     /translation="MENFDKVKDIIVDRLGVDADKVTEDASFKDDLGADSLDIAELVM
                     ELEDEFGTEIPDEEAEKINTVGDAVKFINSLEK"
     gene            19927..20658
                     /locus_tag="O0293_000227"
     CDS             19927..20658
                     /locus_tag="O0293_000227"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCY0279140.1"
                     /translation="MSKQKKSEIVNRFRKRFDTKMTELGFTYQNIDLYQQAFSHSSFI
                     NDFNMNRLDHNERLEFLGDAVLELTVSRYLFDKHPNLPEGNLTKMRATIVCEPSLVIF
                     ANKIGLNEMILLGKGEEKTGGRTRPSLISDAFEAFIGALYLDQGLDIVWKFAEKVIFP
                     HVEQNELLGVVDFKTQFQEYVHQQNKGDVTYNLIKEEGPAHHRLFTSEVILQGEAIAE
                     GKGKTKKESEQRAAESAYKQLKQIK"
     gene            20805..24371
                     /gene="smc"
                     /locus_tag="O0293_000228"
     CDS             20805..24371
                     /gene="smc"
                     /locus_tag="O0293_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HCY0279141.1"
                     /translation="MVYLKSIDAIGFKSFADQTNVQFDKGVTAIVGPNGSGKSNITDA
                     IKWVLGEQSAKSLRGSKMEDIIFSGAEHRKAQNYAEVQLRLDNHSKKLSVDENEVIVT
                     RRLYRSGESEYYINNDRARLKDIADLFLDSGLGKEAYSIISQGRVDEILNAKPIDRRQ
                     IIEESAGVLKYKKRKAESLNKLDQTEDNLTRVEDILYDLEGRVEPLKEEAAIAKEYKT
                     LSHQMKHSDIVVTVHDIDQYTNDNRQLDQRLNDLQGQQANKEADKQRLSQQIQQYKGK
                     RHQLDNDVESLNYQLVKATEAFEKYTGQLNVLEERKKNQSETNARYEEEQENLMELLE
                     NISNEISEAQDTYKSLKSKQKELNAVIRELEEQLYVSDEAHDEKLEEIKNEYYTLMSE
                     QSDVNNDIRFLKHTIEENEAKKSRLDSRLVEVFEQLKDIQGQIKTTKKEYQQTNKELS
                     AVDKEIKNIEKDLTDTKKAQNEYEEKLYQAYRYTEKMKTRIDSLATQEEEYTYFFNGV
                     KHILKAKNKELKGIYGAVAEIIDVPSKLTQAIETALGASLQHVIVDSEKDGRQAIQFL
                     KERNLGRATFLPLNVIQSRVVATDIKSIAKEANGFISIASEAVKVAPEYQNIIGNLLG
                     NTIIVDHLKHANELARAIKYRTRIVTLEGDIVNPGGSMTGGGARKSKSILSQKDELTT
                     MRHQLEDYLRQTESFEQQFKELKIKSDQLSELYFEKSQKHNTLKEQVHHFEMELDRLT
                     TQETQIKNDHEEFEFEKNDGYTSDKSRQTLSEKETYLESIKASLKRLEDEIERYTKLS
                     KEGKESVTKTQQTLHQKQSDLAVVKERIKTQQQTIDRLNNQNQQTKHQLKDVKEKIAF
                     FNSDEVMGEQAFQNIKDQINGQQETRTRLSDELDKLKQQRIELNEQIDAQEAKLQVCH
                     QDILAIENHYQDIKAEQSKLDVLIHHAIDHLNDEYQLTVERAKSEYTSDESIDALRKK
                     VKLMKMSIDELGPVNLNAIEQFEELNERYTFLSEQRTDLRKAKETLEQIISEMDQEVT
                     ERFKETFHAIQGHFTAVFKQLFGGGDAELQLTEADYLTAGIDIVVQPPGKKLQHLSLL
                     SGGERALTAIALLFAILKVRSAPFVILDEVEAALDEANVIRYAKYLNELSDETQFIVI
                     THRKGTMEFADRLYGVTMQESGVTKLVSVNLNTIDDVLKEEQ"
     gene            24371..25621
                     /gene="ftsY"
                     /locus_tag="O0293_000229"
     CDS             24371..25621
                     /gene="ftsY"
                     /locus_tag="O0293_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCY0279142.1"
                     /translation="MSFFKRLKDKFATNKENEEVKSLTEEQGQDKLEDTHSEGSTQDA
                     NDLAENAEVKKKPRKLSEADFDDDGLISIEDFEEIEAQKMGAKFKAGLEKSRQNFQEQ
                     LNNLIARYRKVDEDFFEALEEMLITADVGFNTVMTLTEELRMEAQRRNIQDTEDLREV
                     IVEKIVEIYHQEDDNSEAMNLEDGRLNVILMVGVNGVGKTTTIGKLAYRYKMEGKKVM
                     LAAGDTFRAGAIDQLKVWGERVGVDVISQSEGSDPAAVMYDAINAAKNKGVDILICDT
                     AGRLQNKTNLMQELEKVKRVINRAVPDAPHEALLCLDATTGQNALSQARNFKEVTNVT
                     GIVLTKLDGTAKGGIVLAIRNELHIPVKYVGLGEQLDDLQPFNPESYVYGLFADMIEQ
                     NEEITTVENDQIVTEEKDDNHGSK"
     gene            25608..25940
                     /locus_tag="O0293_000230"
     CDS             25608..25940
                     /locus_tag="O0293_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HCY0279143.1"
                     /translation="MGQNDLVKTLRMNYLFDFYQSLLTNKQRNYLELFYLEDYSLSEI
                     ADTFNVSRQAVYDNIRRTGDLVEDYEKKLELYQKFEQRREIYDEMKQHLSNPEQIQRY
                     IQQLEDLE"
     gene            25966..27333
                     /gene="ffh"
                     /locus_tag="O0293_000231"
     CDS             25966..27333
                     /gene="ffh"
                     /locus_tag="O0293_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002457378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCY0279144.1"
                     /translation="MAFEGLSERLQATMQKMRGKGKLTEADIKIMMREVRLALLEADV
                     NFKVVKEFIKTVSERALGSDVMQSLTPGQQVIKIVQDELTQLMGGENTSINMSNKPPT
                     VVMMVGLQGAGKTTTAGKLALLMRKKYNKKPMLVAADIYRPAAINQLQTVGKQIDIPV
                     YSEGDQVKPQQIVTNALKHAKEEHLDFVIIDTAGRLHIDEALMNELKEVKDIAKPNEI
                     MLVVDSMTGQDAVNVAESFDDQLDVTGVTLTKLDGDTRGGAALSIRSVTQKPIKFVGM
                     SEKLDGLELFHPERMASRILGMGDVLSLIEKAQQDVDQEKAKDLEKKMRESSFTLDDF
                     LEQLDQVKNLGPLDDIMKMIPGMNKMKGLDKLNMSEKQIDHIKAIIQSMTPAERNNPD
                     TLNVSRKKRIAKGSGRSLQEVNRLMKQFNDMKKMMKQFTGGGKGKKGKRNQMQNMLKG
                     MNLPF"
     gene            27768..28043
                     /gene="rpsP"
                     /locus_tag="O0293_000232"
     CDS             27768..28043
                     /gene="rpsP"
                     /locus_tag="O0293_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCY0279145.1"
                     /translation="MAVKIRLTRLGSKRNPFYRIVVADARSPRDGRIIEQIGTYNPTS
                     ANAPEIKVDEALALKWLNDGAKPTDTVHNILSKEGIMKKFDEQKKAK"
     gene            28231..28734
                     /gene="rimM"
                     /locus_tag="O0293_000233"
     CDS             28231..28734
                     /gene="rimM"
                     /locus_tag="O0293_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCY0279146.1"
                     /translation="MRVEVGQIVNTHGIKGEIKVKSNSDFTDVRFQPGQVLTVVHNNN
                     DLEYTVKSHRVHKGLHMLTFEGINNINDIEHLKGSSIYQERDHEDIVLEENEFYYSDI
                     IGCTVFDDQETPIGRVINIFETGANDVWVIKGSKEYLIPYIADVVKEVDVENKKIIIT
                     PMEGLLD"
     gene            28734..29471
                     /gene="trmD"
                     /locus_tag="O0293_000234"
     CDS             28734..29471
                     /gene="trmD"
                     /locus_tag="O0293_000234"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCY0279147.1"
                     /translation="MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAIN
                     KHNQVDDYPYGGGQGMVLKPEPVFNAMEDLDVTEQTRVILMCPQGEPFSHQKAVELSK
                     ADHIVFICGHYEGYDERIRTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGN
                     EQSHQDDSFSDGLLEFPQYTRPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKR
                     PDLIEKYPLTNADKQILERYKIGLKKG"
     gene            29574..29924
                     /gene="rplS"
                     /locus_tag="O0293_000235"
     CDS             29574..29924
                     /gene="rplS"
                     /locus_tag="O0293_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCY0279148.1"
                     /translation="MTNHKLIEAVTKSQLRTDLPSFRPGDTLRVHVRIIEGTRERIQV
                     FEGVVIKRRGGGVSETFTVRKISSGVGVERTFPLHTPKIEKIEVKRRGKVRRAKLYYL
                     RSLRGKAARIQEIR"
     gene            complement(30168..32774)
                     /gene="yfhO"
                     /locus_tag="O0293_000236"
     CDS             complement(30168..32774)
                     /gene="yfhO"
                     /locus_tag="O0293_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosyltransferase
                     YfhO"
                     /protein_id="HCY0279149.1"
                     /translation="MLKKWLNSNVKQFFVITFISVILTLILFSTHIYDYIVNGTVFSG
                     AGDGFRQMMPFQMYLYEHLRSFSSLYDASFGLGGDYMKGLSYYYSLSPLMWLNFLFIK
                     IGETVGIFNPTTIHFWPTNQLIMAMIRAIITFVVTFYLFKILHFKRSANMIATILYGM
                     STVVIYFNFTWSFYGNLLYLLPLSILGLERYFQQRKIGIFIVAIALTLFSNFYFSYYQ
                     AIIIGCYYLYRLIFTYKYDIVSRTQKLICVISATVLSVLSSVFGLFTGISAFLENDRK
                     QNPNVDIPFLTPLDYHYFFFSDGFYITISILTIVALLSFKLYRFYFYRLFAIVTWILF
                     IGSLSQYFDSAFNGFSFPERRWVYILALSSSALCGLFIQHLSTLNMKYYLIRTIPVCI
                     IAILYVLLSPTHPLALIVGIILLIVLAVILKFSLWRYKKLTVAILVLIVMIQQIVILD
                     NNKNMAIKPYQQSLSTLKQHDYHSNYVNQLIKKINQNATGSFNRIDYMSDYALNSPFI
                     YHYNGISLYSSIFNGDILKYYDKTLQINMPIDKNSTYRLLGNRQNLLSLWNVNDRIRV
                     NHDDNLPYGFKIKSEHKDNKVRWIHSKNTIHYPSAHITNKVFSNKELKSPLDKEQAML
                     QGIVSNNIKDVNTHFKANKNLLSDSTIKLNSAAWQSPTKHLLQVKQNNGGLTVQLPKS
                     VSNQFKDLYFEMDLELLSPDKAHDVKVNEYTQERNKLTYKYRRVVTPVTIRIKAPDRI
                     RLSLPKGKYRVNLKGIYGEDYTTLKDASNSLEAVKVSKTKHGYTITKNKNSSGYIVLP
                     TAYNQGMKATSGDQSLKVEQVNGVMTGIKAPKNITKIQLSYTPPYYYLLITITIFGII
                     CSIIFTRWARQK"
     gene            33175..34059
                     /gene="ylqF"
                     /locus_tag="O0293_000237"
     CDS             33175..34059
                     /gene="ylqF"
                     /locus_tag="O0293_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HCY0279150.1"
                     /translation="MVIQWYPGHMAKAKREVSEQLKKVDVVFELVDARIPYSSRNPMI
                     DEVINQKPRVVILNKKDMSNLNEMSKWEQFFIDKGYYPVSVDAKHGKNLKKVEAAAIK
                     ATAEKFEREKAKGLKPRAIRAMIVGIPNVGKSTLINKLAKRSIAQTGNKPGVTKQQQW
                     IKVGNALQLLDTPGILWPKFEDEEVGKKLSLTGAIKDSIVHLDEVAIYGLNFLIQNDL
                     ARLKSHYNIEVPEDAEIIAWFDAIGKKRGLIRRGNEIDYEAVIELIIYDIRNAKIGNY
                     CFDIFKDMTEELANDANN"
     gene            34043..34810
                     /locus_tag="O0293_000238"
     CDS             34043..34810
                     /locus_tag="O0293_000238"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCY0279151.1"
                     /translation="MTLTIKEVTQLINAVNTIEELENHECFLDERKGVQNAIARRRKA
                     LEKEQALKEKYVEMTYFENEILKEHPNAIICGIDEVGRGPLAGPVVACATILNSNHNY
                     LGLDDSKKVPVTKRLELNEALKNEVTAFAYGIATAEEIDEFNIYKATQIAMQRAIDGL
                     SVQPTHLLIDAMTLDNALPQVSLIKGDARSVSIAAASIMAKVFRDDYMTQLSKDYPEY
                     GFEKNAGYGTKQHLLAIDDIGIMKEHRKSFEPIKSLL"
     gene            34919..36085
                     /gene="sucC"
                     /locus_tag="O0293_000239"
     CDS             34919..36085
                     /gene="sucC"
                     /locus_tag="O0293_000239"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCY0279152.1"
                     /translation="MNIHEYQGKEIFRSMGVAVPEGRVAFTAEEAVEKAKELNSDVYV
                     VKAQIHAGGRGKAGGVKIAKSLSEVETYAKELLGKTLVTHQTGPEGKEIKRLYIEEGC
                     AIQKEYYVGFVIDRATDQVTLMASEEGGTEIEEVAAKTPEKIFKETIDPVIGLSPFQA
                     RRIAFNINIPKESVNKAAKFLLALYNVFIEKDCSIVEINPLVTTADGDVLALDAKINF
                     DDNALFRHKDVVELRDLEEEDPKEIEASKHDLSYIALDGDIGCMVNGAGLAMATMDTI
                     NHFGGNPANFLDAGGSATREKVTEAFKIILGDENVKGIFVNIFGGIMKCDVIAEGIVE
                     AVKEVDLTLPLVVRLEGTNVELGKKILKDSGLAIEPAATMAEGAQKIVKLVKEA"
     gene            36107..37015
                     /gene="sucD"
                     /locus_tag="O0293_000240"
     CDS             36107..37015
                     /gene="sucD"
                     /locus_tag="O0293_000240"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCY0279153.1"
                     /translation="MSVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVAGVTPGKG
                     GQVVEGVPVFNTVEEAKNETGATVSVIYVPAPFAADSILEAADADLDMVICITEHIPV
                     LDMVKVKRYLQGRKTRLVGPNCPGVITADECKIGIMPGYIHKKGHVGVVSRSGTLTYE
                     AVHQLTEEGIGQTTAVGIGGDPVNGTNFIDVLKAFNEDDETKAVVMIGEIGGTAEEEA
                     AEWIKANMTKPVVGFIGGQTAPPGKRMGHAGAIISGGKGTAEEKIKTLNSCGVKTAAT
                     PSEIGSTLIEAAKEAGIYESLLTVNK"
     gene            37242..>37847
                     /locus_tag="O0293_000241"
     CDS             37242..>37847
                     /locus_tag="O0293_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCY0279154.1"
                     /translation="MNKQQSKVRYSIRKVSIGILSISIGMFLALGMSNKAYADEIDKS
                     KDFTRGYEQNVFAKSELNANKNTTKDKIKNEGAVKTSDTSLKLDNKSAISNGNEINQD
                     IKISNTPKNSSQGNNLVINNNELTKEIKIANLEAQNSNQKKTNKVTNNYFGYYSFREA
                     PKTQIYTVKKGDTLSAIALKYKTTVSNIQNTNNIANPNLIFI"
BASE COUNT        14086 a         5107 c         7284 g        11370 t
ORIGIN      
        1 aatttatgta tgtttatttg attcaaacaa aataacttaa gaggagaaag tttatgaaaa
       61 agacactggg atgtttactt ttaattatgc ttttagtcgt agcaggttgt tcttttggtg
      121 ggaatcataa attatcatca aagaaatcag aagaatcaaa acaagaaact gtaaaaaaag
      181 aatcggaaga agagaaagat ccagatttag agaaatatga agaaatagag aagaaaatga
      241 aaggaattaa agatgcgcca tctcttgata agttggatcc attaatgaca gaaaagtcgt
      301 ttacgaatag taaagggatt caaggatgga aagattacaa agaattaatg ggtaaagtgg
      361 aacttgcaga ttatagattt actaaagatt caaaaggatc ttcaataaaa gatgttgatg
      421 cattctttaa aggtaagaaa ggtataaaaa ggaaagtgat tgaaacacac gatgatgtaa
      481 aacaagttga ttattggtat gtagatccag atggaaagaa aattggcaat tcaaacacac
      541 ctgtttttta cgcagaaatt atgacaaaat ataaagatgg aaagttagtt tatgcatcag
      601 tcgaaccagg atcttacgta atacataaag atgatgcaat taaatatgac gattattcta
      661 agttaaaaaa attaagtcag ctaactaaac ttgatcatcc aaaaccagtt ccatatagcg
      721 tagctcaaat caaatctttc ggagtacctt taacaagcgt ttcatttatg acacatggat
      781 caaaggatac taaagatgaa gtgttgccgg cattggccta tttcactttt tcaccaaaaa
      841 attatgaaga caagtctaat ccagatccaa aagttttaaa tttagtacat atggatttct
      901 taaatgcatc tagtgatttt ggtaacgcac attttgttgt tttaagtaaa tatattaaag
      961 agtatgaatc aaactatgaa acagcgtcag atgattcttt aaaatagtat ttactgtgtg
     1021 aaaaataaat agtgtactac attaaataat cgcaataata atcccgataa acaatcagca
     1081 ttactgctta tcacatagag ttcgtaataa ctataactct atgattcgca aataataaat
     1141 gattgtcatc gggatttatt tttatcaatt tataaagtga cattaccttg ttcatcagca
     1201 ggtttgaaaa cagtaatcac tgcactaata attgctaaaa tgtgtgggat acctgtccaa
     1261 cagaatatta agtgtagaat accttgcata ttcttgccgg cataaaattt atgaatacca
     1321 aaactaccta agaacaatgc taataaaata taaataactt tgtttacttg catttctttc
     1381 cctccagttg aattgcttat aatgacatta gcttctcttt ttattatacc cacttttagt
     1441 tcaaacattc tagtttaagc attcccaatc atctaaattt cagttattca atccttacaa
     1501 taaatttagg attacatttc agttgcattg tattatttta cgtgtgaaat atacgtaatg
     1561 aatcacatga caatcttcaa attgaaaaat atacattcta tgatgtaagg tcgcattttt
     1621 aatatattta cgttataata gtttgatgaa ttttattgag gagtaagagt tatggcgatt
     1681 aaaaagttag taccagcatc gcatcctatt ttaacgaaaa aagcgcaagc agttaaaaca
     1741 tttgatgatt cgttaaaaag attattacaa gatttagaag atacaatgta tgcacaagaa
     1801 gctgctggct tatgtgcacc tcaaattaat cagtcattgc aagtggcaat tattgatatg
     1861 gaaatggaag gattattaca acttgttaat ccgaaaatta ttagtcaatc aaatgaaaca
     1921 ataacagact tagaaggttc aattacattg ccagatgttt acggcgaagt gacaagaagt
     1981 aaaatgatag ttgtcgaaag ttatgacgtc aatgggaaca aagttgaact aactgcacat
     2041 gaagatgtag caagaatgat tttgcatatt atagatcaaa tgaacggtat cccttttaca
     2101 gaacgtgcgg accgtatttt aacagataaa gaagtggagg catattttat aaatgactaa
     2161 aataatattt atgggtacac cagacttttc aacaactgtt ttagaaatgc ttattgcaga
     2221 acatgatgtc attgcagtcg taacgcaacc agatcgacct gttggacgta aacgtgttat
     2281 gacaccacca ccagttaaaa aagttgcaat gaaatatgat ttacctgtat atcaacctga
     2341 aaaattaagt ggatcagaag aattagaaca attgcttcaa ttagatgtag atttaattgt
     2401 aactgctgct tttggacaat tattacctga atcattgttg gcattaccaa atcttggggc
     2461 aattaatgta catgcatcat tgttaccgaa gtatagaggt ggtgcaccaa ttcatcaggc
     2521 aattatcgat ggtgaacaag aaaccggcat aacaattatg tatatggtta aaaaattaga
     2581 tgcgggtaat attatttcgc aacaagcaat taaaatagaa gaaaatgata atgtcggtac
     2641 gatgcatgat aaattaagtg tattaggggc agatttatta aaagaaactt taccatctat
     2701 tatagagggc acaaatgaaa gcgtacctca agatgatacg caagcaacat ttgcttccaa
     2761 tattcgacgc gaagatgagc gaattagctg gaataaacca ggaagacaag tgtttaatca
     2821 aattcgtgga ttatcaccat ggccagttgc ttatacaact atggatgaca ctaacttgaa
     2881 aatatacgat gctgaactcg ttgagactaa taagataaac gagcctggaa ccattataga
     2941 aacgactaaa aaagccatta ttgttgctac aaatgataat gaagctgttg caattaaaga
     3001 tatgcaatta gctgggaaaa agagaatgtt agctgccaat tatttaagtg gtgcgcaaaa
     3061 cacactagta gggaagaaac ttatatgata gaaaacgtga gaagtcttgc ttttgacacg
     3121 attcaagata tattaaatga aggtgcgtat agtaacttgc gtatcaatga agtgttgtca
     3181 gaaaatgaat taaatgcaat ggataaggct ttatttacag aaattgtcta cggaaccgtt
     3241 aaaagaaaat atacgttaga tttttattta aagccttttg tgaaaacaaa gattaaggca
     3301 tgggttaggc aattattatg gatgagtatt tatcaatatg tttatttaga taaagttcca
     3361 aatcatgcca ttattaatga agcagttgaa atagcaaaag aacgcggtgg ctatcataat
     3421 ggtaatgtcg taaatggtat tttacgtaca atgatgcgta gtgacttacc tgattttaat
     3481 gaaattgcag atcctaaaaa aagaatggca atcgagtata gtatgccgaa gtggattata
     3541 gatcattggg caacacatta tggtctcgaa gaaactgaaa caattttaca gtcattttta
     3601 gaaacgacat caacaactgt gcgtgccaac ctgacgcgag catcattaga tgatattatt
     3661 gaaaagttgc aagacgaagg ttatgacgtt gaaaaagatc atgacttacc ttattgtctc
     3721 catataggag gacaaccaat tattcattct cgttcattta aagatggatt cgtttcaatt
     3781 caagataaaa gctcaatgtt tgttgcacac attatgaatg tagaccgaca tgatcacgta
     3841 ttagatgcat gtagtgcacc tggcggtaaa gcttgtcaca ttgctgaagt tttaatgcca
     3901 gaagggcaag ttgacgcttc agatatacat gatcacaaaa tagacttaat taattttaat
     3961 ataaaaaaat tacgattaac aaatattaaa gcttttcaac atgatgcgac aaaaccttat
     4021 gataaaacat acgataagat acttgttgat gcaccatgta gcggattagg tgtaatgaga
     4081 cataagccgg agattaagta tactcaaagc aaacaacata ttgagtcact agttgaatta
     4141 cagcttgaaa tattggaaaa tgtaaaaaac aatgtaaaaa taggtggcga aatcatctat
     4201 tcaacatgta caattgagca actagaaaat gaaaacgtga tttatacgtt tttgaaaaat
     4261 aataaaaact tcgaatttga accgtttcaa catccgataa ctggagagtt ggtcaaaacg
     4321 ttacaaatca tgccgcaaga ctttaattca gatggattct ttatcactaa gataaaaaga
     4381 aaggacaatt aggaatgata actgctgaaa agaaaaagaa gaataaattt cttccaaatt
     4441 ttgacaagca atcaatatat tcattgcgat ttgacgaaat gcaaaactgg ctcgttgaac
     4501 aagggcaaca aaaatttcga gcgaaacaga tttttgaatg gttatatcaa aaaagagtag
     4561 attcgattga tgaaatgacg aacttatcga aagacttacg acagctttta aaagataact
     4621 ttactgttac aactttaaca actgtagtaa aacaagaaag taaagacggt acaattaaat
     4681 tcttatttga attacaagat ggctatacaa ttgaaactgt tttaatgaga catgattatg
     4741 gaaattcagt atgtgtaacg acacaagtag gttgtcgcat cggatgtacg ttttgtgctt
     4801 ctacacttgg cggcttaaaa agaaaccttg aagctggcga aattgtttca caagttttaa
     4861 cagttcaaaa agcccttgat gctacagaag agcgcgtatc tcaaattgtc ataatgggta
     4921 tcggtgaacc atttgaaaat tatgatgaaa tgatggactt tttaagaatc gtcaatgatg
     4981 ataacagttt aaatattggt gcacgtcaca ttacagtatc aacatcaggt atcattccta
     5041 gaatatatga ctttgcggat gaagatattc aaattaattt tgctgtaagc ttacacgccg
     5101 caaaagatga agtgcgatca cgcttgatgc caattaaccg tgcatataat gttgagaagt
     5161 taatcgaagc aattcaatat tatcaagaaa aaacaaatcg tcgtgttact tttgaatatg
     5221 gtctgtttgg tggtgtgaat gaccaactag aacatgcaag agaattagca catttaataa
     5281 aaggcttaaa ctgccatgtt aacttaattc ctgtcaacca tgttccagaa agaaattatg
     5341 tgaaaacggc taaaaatgat atctttaaat ttgaaaaaga attaaagaga ctaggaatta
     5401 atgccacaat acgtcgtgaa caaggttcgg atattgacgc agcttgtggt caattaagag
     5461 caaaggaacg acaagtagaa acgaggtaaa gacaaatgct agaggcacaa ttttttactg
     5521 atactggaca acatagagat aagaatgaag atgcgggtgg tattttttat aatcaaacta
     5581 atcaacaact tttagttctg tgtgatggta tgggtggcca taaagcagga gaagttgcaa
     5641 gtaaatttgt tacagatgag ttgaaatccc gttttgaagc ggaaaatctt atagaacaac
     5701 atcaagctga aaattggttg cgtaataata taaaagatat aaattttcag ttatatcact
     5761 atgcacaaga aaatgcagaa tataaaggta tgggtacaac atgtgtttgt gcacttgttt
     5821 ttgaaaaatc agttgtgata gcaaatgtcg gtgattctag agcctatgtt attaatagtc
     5881 gacaaattga acaaattact agtgatcact catttgttaa tcatcttgtt ttaacgggtc
     5941 aaattacgcc ggaagaagca tttacacatc cacaacgtaa tattattacg aaggtgatgg
     6001 gcacagataa acgtgtgagt ccagatttgt ttattaagcg attaaatttt tatgattatt
     6061 tattattaaa ttcagatgga ttaactgatt atgttaaaga caatgaaatt aagcgtttgt
     6121 tagtaaaaga aggtacaata gaagatcatg gtgatcaatt aatgcaattg gcattagata
     6181 accattcgaa agataacgtt actttcatac tcgcggctat tgaaggtgat aaagtatgat
     6241 aggtaaaata ataaatgaac gatataaaat tgtagataag cttggcggcg gtggcatgag
     6301 taccgtttat cttgctgaag atacgatact taacattaaa gttgcaatta aggcgatttt
     6361 tataccacct agagaaaaag aagaaacatt aaaacgtttt gaacgagaag tacataactc
     6421 atcacagcta tcacatcaaa atatagtaag tatgatcgat gttgatgaag aagatgactg
     6481 ttactactta gtaatggaat atatcgaagg tccgactttg tctgagtata ttgaaagtca
     6541 tgggccatta agtgttgaca cagcgattaa ttttacgaat caaatattgg atggcattaa
     6601 acatgcgcat gatatgcgta ttgtacatag agatattaag ccacaaaata tattaattga
     6661 cagcaataaa acgttgaaaa tatttgattt tggaattgct aaagctttaa gtgagacgtc
     6721 tttaactcag actaatcatg tgttaggtac tgtgcagtac ttttcgccag aacaagcaaa
     6781 aggtgaggca acggatgaat gtacagatat ttattctata ggtattgtgt tatatgaaat
     6841 gcttgttggt gaaccaccct ttaatggaga aactgcagtt agcattgcga ttaaacatat
     6901 tcaggattct gtgccaaatg tgacaacaga tgtacgtaag gatattccgc aatctttaag
     6961 taatgtcatt ttacgcgcta cagaaaaaga caaagcgaat cgttacaaaa caattcaaga
     7021 aatgaaagat gatttgagta gtgttttaca tgaaaatcga gcgaatgaag atgtctatga
     7081 actcgataaa atgaaaacga tagcggtacc tttgaaaaaa gaagatctag caaagcatat
     7141 tagtgaacat aagtcgaatc aacctaaacg tgaaacgacg caagtaccta ttgtaaatgg
     7201 gcctgctcat catcagcaat tccaaaagcc agaaggtacg gtgtacgaac caaaacctaa
     7261 aaagaaatca acacgaaaga ttgtgctctt atcactaatc ttttcgttgt taatgattgc
     7321 acttgtttct tttgtggcaa tggcaatgtt tggtaataaa tacgaagaga cacctgatgt
     7381 aatcgggaaa tctgtaaaag aagcagagca aatattcaat aaaaacaacc tgaaattggg
     7441 taaaatttct agaagttata gtgataaata tcctgaaaat gaaattatta agacaactcc
     7501 taatactggt gaacgtgttg aacgtggtga cagtgttgat gttgttatat caaagggccc
     7561 tgaaaaggtt aaaatgccaa atgtcattgg tttacctaag gaggaagcct tgcagaaatt
     7621 aaaatcgtta ggtcttaaag atgttacgat tgaaaaagta tataataatc aagcgccaaa
     7681 aggatacatt gcaaatcaaa gtgtaaccgc aaatactgaa atcgctattc atgattctaa
     7741 tattaaacta tatgaatctt taggcattaa gcaagtttat gtagaagact ttgaacataa
     7801 atcctttagc aaagctaaaa aagccttaga agaaaaaggg tttaaagttg aaagtaagga
     7861 agagtatagt gacgatattg atgagggtga tgtgatttct caatctccta aaggaaaatc
     7921 agtagatgag gggtcaacga tttcatttgt tgtttctaaa ggtaaaaaaa gtgactcatc
     7981 agatgtcaaa acgacaactg aatcggtaga tgtaccatac actggtaaaa atgataagtc
     8041 acaaaaagtt aaagtttata ttaaagataa agataatgac ggttcaactg aaaaaggtag
     8101 tttcgatatt actagtgatc aacgtataga cattccttta agaattgaaa aaggaaaaac
     8161 agcaagttat attgttaaag ttgacggtaa aactgtagct gaaaaagaag tcagctatga
     8221 tgatgtataa atataattga agtaaatgta ccgaggtttc tatttggaag tctcggtatt
     8281 tttatgttga gattgcggta gttttaaaat gcttcatgtc atcatataca ggtgagcatt
     8341 gtctatcaat tctgtatcac tctcacaata tttatatcat ttgaaatgat catttatata
     8401 ttaattttcc agtctttgat ataataaatg cagtaaaatt aatcgagagg tgccattttg
     8461 aagacaggtc gaatagtgaa atcaattagt ggggtatatc aagtagacgt taatggcgaa
     8521 cgtttcaata caaaaccacg aggattattt agaaagaaaa aattttcacc ggtagttggt
     8581 gatatagtgg aatttgaagt tcaaaacatt aacgaaggtt atattcatca agtgtttgag
     8641 cgggaaaatg agttgaaaag accacctgta agtaatatag atacactagt aattgtaatg
     8701 agtgctgtcg agccaaattt ttcaacgcaa ttattagatc gatttttagt tattgcacat
     8761 tcgtatcagt taaatgcgag aattttggtg actaaaaaag ataaaacacc aattgaaaag
     8821 cagttcgaaa taaatgagtt gttgaaaata tatgaaaata ttggctatga gactgaattt
     8881 attggaaatg atgatgatcg aaaaaaaatt gtagaagctt ggccagctgg acttatagta
     8941 cttagtggtc aatcaggtgt cggtaagtcc actttcttaa atcattatcg tccagaactt
     9001 aatcttgaga caaatgatat atcaaaatca ttaaatcgag gaaagcatac tacaagacat
     9061 gtcgaactat tcgaacgtca aaacggttat attgcagaca cacctggatt cagtgcttta
     9121 gattttgatc atatagataa agatgaaata aaagattatt ttcttgaatt aaatcgatat
     9181 ggtgaaacat gtaagtttag gaattgtaat catatcaaag aacctaattg taatgttaag
     9241 catcaattag agatagggaa tattgcgcaa tttagatacg accattattt acaactattt
     9301 aatgaaattt caaatagaaa ggttagatat taaatgacaa aactatatcc atcattatta
     9361 tctgttgatt ttttggattt acaacatgaa ttaaaacgac ttgaagaagc aggcgtcgac
     9421 ggagttcatt ttgatgttat ggatggtcaa tttgtgccta atatatctat tggtttacca
     9481 atattagatg cagtaagaaa aggcacaaca ttacctatag acgtacattt gatgattgaa
     9541 aatccagaaa agtatattgc atcatttgca gaacatggtg ccgatatgat ttcaattcat
     9601 gtcgaatcaa cgcctcatat tcatcgtgct attcaaatga ttaaacattt agataaaaaa
     9661 gctggtgtag taattaatcc tggtacacca atatcacaaa ttgaacctat tttagacatt
     9721 gttgattatg tactagtgat gacagttaac ccagggtttg gtggtcaatc atttattgat
     9781 caatgcgtag aaaaaatagc gggtcttaat gctattaaaa tggaacgtca attaaacttt
     9841 gatattgaag ttgatggagg cgtaaatacc gatacagcga aagtttgtgt tgaaaatggt
     9901 gctacaatgc tagtaacagg ttcatttttc tttaaacaag aggattataa aaaagtcaca
     9961 caagcattga aaggttgagt gtatatgcat ataaatttat tatgttctga tcgacacttg
    10021 ccgcaagata tttgggccaa aagtaatgaa ggtaaatggg gcggcgttga tagaggtgct
    10081 ttgattttat tgaagcatca aattatccct tttttctcag tgggagactt tgattcagtc
    10141 agtaaagaag aacgccaact tctaacagaa cagttacaaa tcaaaccagt tcaagctgaa
    10201 aaagctgata cggatttagc tttagcggtt gataaagctg ttgcacttgg atttgatagt
    10261 attacaattt atggtgcaac aggcggacga ttagatcact tttttggggc aattcagtta
    10321 ttattgaaaa aagcatatta taaacatgat gttcatatag aagttatcga tcaacaaaat
    10381 aaaattgaat tattgcctaa aggtcaacat acagttgaaa aagataagag ttatccgtac
    10441 atttcattta taccgatgac tgatgatgta gaactttctc tagcaggttt taaatataat
    10501 ttagctagac aaatgcttaa tataggttct actttaacta tttcaaatga aattgagtct
    10561 ttgcaagcga aagtaactgt acatgatggg ttgattttgc aaattagaag tacagattta
    10621 aattaagtat attcaaattt gtgaggattt ttaataaagt gcatgagtta tttaataaag
    10681 tgacgtttat agatagaatt tataacataa taatagatga atcacgctgt aattaatgaa
    10741 gattatggcg tgatttatta ttgaaaggaa aattgtaggt cctagtacat tgatttatgt
    10801 gccaaaatcg atattgaacc cataagtcca atttgacaga gaaccattat taaacgtatt
    10861 aaccaaatta tattgatagt tcgaatttca ctatgaacca ttatgtattg tttggtaaca
    10921 aaaaaagaac taagaggttg agctcttagt tcttaagtaa atgtgtcctc cacataaggt
    10981 cacatttttt atgtgcaata tatttattaa actctagtta ctttaccaga ttttaaagca
    11041 cgtgcagaaa cccaaacttt tttaggttta ccgtcaacta ggattctaac tttttgaagg
    11101 ttagcgttcc atctacgttt agtagagttt aaagcgtgtg aacgtctgtt accagtcgaa
    11161 gctttacgac ctgttacgaa acattgttta cccatatgag tacctccttt aataaatata
    11221 aatacacata actacttata tacttaatta agatagcata gtttcatttg aaaaacaatg
    11281 aataattttc acataaaagt caaaaatact gagttttgtg atataattgt agactgtgaa
    11341 gttatgtagt atgatatttt agagaaataa gtgaatgaaa catttaaaat ttttatttaa
    11401 tgatactaca tctattaaaa gaaaacgcct atagataatc gtcatgagtg taaattcagt
    11461 ttactcaaaa tataataggt gatttaagcg taagtcattc tgataagttg taccattgat
    11521 attttataaa aataatcaat gaaacttaaa gcgtttatga ctacactaag tggtaaaatt
    11581 ttaaaagtga ttagggaaaa cgaataataa actttgaaat ttaggaggga caaagatatg
    11641 acattagaga tttcaaatga ttacggtaaa attgatattt caaacgaagt gattgcttcg
    11701 gttgtaggtg gaaaggccgt tgaatgttat ggtattgtag gtatggcatc tagacaacaa
    11761 gttagagatg gtattgcgga aatactagga catgaaaatt atgctaaagg catcaaagta
    11821 actgaaaata atggcgtagt ggatatagat atgtacatta ttgtaagtta cggtgtgaaa
    11881 atatctgaag ttgccaataa tgtacaatca acagtgaaat atactttgga aaaatcactt
    11941 aatgtatcag taaattcaat caatatatat gtacaaggtg tacgtgtgaa taatacaggc
    12001 aagaaagctt aggaggacaa cttgaaatga ttagcaaaat taatggtaaa ttatttgccg
    12061 atatgattat acaaggggca caaaatttat ctaacaatgc agatttggta gattctttga
    12121 atgtgtatcc agtgccagat ggtgatacag gaacaaatat gaatcttact atgacttcag
    12181 gtcgcgaaga agtagagaat aatttgtcga aaaatatcgg cgaattaggt aaaacattct
    12241 cgaaaggttt actaatgggt gcaagaggta actctggtgt catcttgtca caattattca
    12301 gaggattttg taaaaatatt gaaagtgaat ctgaaattaa ttcaaaattg ttagctgaaa
    12361 gttttcaagc tggtgttgaa acggcatata aagctgttat gaaaccagtt gaaggtacaa
    12421 tacttacagt tgcaaaagat gctgcgcaag ctgcaataga aaaagcaaat aatactgaag
    12481 attgtataga attaatggag tacattattg taaaagccaa tgaatcactt gaaaacacac
    12541 caaacttatt agctgtactt aaagaagttg gtgttgttga tagtggcggt aaaggtttgt
    12601 tatgcgttta cgaaggattc ttaaaagcgc ttaaaggtga aaaagttgaa gccaaagttg
    12661 caaagataga taaagatgaa tttgtacatg atgaacatga tttccatggt gtaattaata
    12721 ctgaagatat tatttatggc tattgtactg aaatgatggt tcgttttgga aagaataaaa
    12781 aagcctttga tgaacaagaa ttcaggcaag atatgagtca atttggtgat tctttattag
    12841 tcattaatga tgaagaaatt gtgaaagttc acgtgcatac cgaataccca ggtaaagtgt
    12901 ttaattatgg tcaacaatat ggtgaattaa ttaaacttaa ggttgaaaat atgagagaac
    12961 agcaccgtga agtgattcga aaagaacagc acacagctaa accgaaaatg gaaacggttg
    13021 aaacagcaat tattactatt tctatgggtg aaggtatttc agagatattt aaatcaatgg
    13081 gtgccacaca tatcattagt ggtggacaaa cgatgaatcc ttctacagaa gatatcgtta
    13141 aagtcattga acaatcaaaa tgtaaacgtg caattatttt accgaataat aaaaatatct
    13201 taatggcaag tgaacaagca gcgagtattg ttgatgcaga agctgttgtt attccaacga
    13261 aatctattcc tcaaggtata agcgcactat tccaatatga tgtggacgca acacttgaag
    13321 aaaataaagc gcaaatggct gattcagtaa ataacgttaa atctggttca ttaacgtacg
    13381 ctgttcgtga tacgaaaatt gatggcgttg agattaaaaa agacgcgttt atgggcttga
    13441 ttgaagataa gattgtaagc agccaaagtg atcaattaac aacggttact gagttgttaa
    13501 atgagatgtt agcagaagat agtgaaatat tgactgtgat tattggtcaa gatgcagagc
    13561 aagcagttac agataacatg ataaactgga tcgaagagca atatccagat gtagaagtgg
    13621 aagttcatga aggtggacaa ccaatttatc aatatttctt ttcagtagaa taaaaattta
    13681 aaataaaaaa ctaccaatga taaatcatca gttggtagtt ttttattttg ctattttagt
    13741 gatattgcgg gttaaaagta tcgttctcga gttgctaaca atgtcatgtt caacttagtc
    13801 atgataaaat aaataacata ctaaatgata cgtaaaatca aataaaacat aggtgattta
    13861 ttttggctaa agtaaactta atagaaagtc catattctct tttacaatta aaaggtatag
    13921 gtcctaagaa aatagaagta ttgcaacaac taaatattca tacagtggaa gatcttgttc
    13981 tttatttgcc aactagatat gaagataata cagtgattga tttgaatcaa gcagaagatc
    14041 aatctaacgt tacgatagaa ggacaagtat atacagctcc agtagttgca ttttttggaa
    14101 gaaataaatc aaaattaacc gttcatttaa tggtaaataa tattgctgtc aaatgtattt
    14161 ttttcaatca accgtattta aaaaagaaaa tcgaattaaa tcaaactata actgttaaag
    14221 gtaagtggaa tagggttaaa caggaaatta ctggtaatag ggttttcttt aattcacaag
    14281 ggacacaaac tcaagaaaac gcagatgttc aattagaacc agtctatcgt attaaggaag
    14341 gtattaaaca aaagcaaata cgagaccaaa ttagacaagc gttaaatgat gtgacaattc
    14401 atgaatggtt aactgatgaa ctaagagaaa aatataaatt agagaccttg gactttactt
    14461 tgaacacatt acatcatcct aaaagtaaag aggatttatt acgtgctcgt agaacctatg
    14521 catttactga actgttttta ttcgaattac gtatgcaatg gctaaataga ttagaaaagt
    14581 catctgacga agcaattgaa attgattatg acatagacca agttaaatca tttattgatc
    14641 gtttaccttt tgaactaact gaagcacaga aatccagtgt taatgaaatt tttagagatt
    14701 taaaagcacc aatacgtatg catcgattac ttcaaggtga tgtaggttca ggaaaaacag
    14761 tagttgctgc aatttgtatg tatgcgttaa aaactgctgg ttatcaatca gcattgatgg
    14821 taccaactga aattttagca gagcaacatg ctgaaagttt aatggcttta tttggagatt
    14881 ctatgaacgt tgcattgtta actgggtcag taaaaggtaa gaaacgaaag atacttttag
    14941 aacaacttga aaatggtacg attgattgtt taattggaac ccatgctttg attcaagatg
    15001 atgtgatttt ccataatgtt ggtttagtaa ttacagatga acaacatcga tttggtgtga
    15061 atcaacgcca gcttttaaga gaaaaaggtg caatgacgaa tgtgttattt atgacagcaa
    15121 cgccgatacc aagaacacta gcaatatcag tttttggtga gatggatgtg tcttcaatta
    15181 aacaattacc aaaaggtcgt aaacctatca ttactacttg ggcaaagcat gagcaatacg
    15241 ataaagtttt gatgcaaatg acctcagagt tgaaaaaagg tcgtcaagca tatgtcattt
    15301 gcccgctaat agaaagttct gagcatctcg aagatgttca aaatgttgtc gcattgtacg
    15361 agtctttaca acagtattat ggtgtttccc gtgtagggtt attgcatggt aagttatctg
    15421 ccgatgaaaa agatgaggtc atgcaaaagt ttagtaatca tgagataaat gttttagttt
    15481 ctactactgt tgttgaagta ggtgttaatg taccgaatgc aacttttatg atgatttatg
    15541 atgcggatcg ctttggatta tcaactttac atcagttacg cggtcgtgta ggtagaagtg
    15601 accagcaaag ttactgtgtt ttaattgcat cccctaaaac agaaacagga attgaaagaa
    15661 tgacaattat gacacaaaca acggatggat ttgaattgag tgaacgagac ttagaaatgc
    15721 gtggtcctgg agatttcttt ggtgttaaac aaagtggatt gccagatttc ttagttgcca
    15781 atttagttga agattatcgt atgttagaag ttgctcgtga tgaagcagct gaacttattc
    15841 aatctggcgt attctttgaa aatacgtatc aacatttacg tcattttgtt gaagaaaatt
    15901 tattacatcg tagttttgac taattgccat gctgatttgt caatttgagt gcaacacttc
    15961 gttaattgag tgatatgaca cttgaactat ttaaatgtaa agtggtattt taacaattta
    16021 taaattttcg actaaataat agctaaatat tacagttatt tgttgagtcg gttaaataga
    16081 aagtgttatg atatgtgagg aatgtttaag actaggtact aaaaaatgag gggtgagacg
    16141 ttgaaactaa agaaagataa acgtagagaa gcaatcagac aacaaattga tagcaatccc
    16201 ttcatcacag accatgaact aagcgactta tttcaagtga gtatacaaac aattcgttta
    16261 gatcgcactt atttaaacat accagaatta aggaagcgta ttaaattagt tgctgaaaag
    16321 aattatgacc aaataagttc tattgaagaa caagaattta ttggtgattt gattcaagtc
    16381 aatccaaatg ttaaagcgca atcaatttta gatattacat cggattctgt ttttcataaa
    16441 actggaattg cgcgtggtca tgtgctgttt gctcaggcaa attcgttatg tgttgcgcta
    16501 attaagcaac caacagtttt aactcatgag agtagcattc aatttattga aaaagtaaaa
    16561 ttaaatgata cggtaagagc agaagcacga gttgtaaatc aaactgcaaa acattattac
    16621 gtcgaagtaa agtcatatgt taaacataca ttagttttca aaggaaattt taaaatgttt
    16681 tatgataagc gaggataaaa ttatggttaa attagcaatt gatatgatgg gtggcgacaa
    16741 tgcgcctgat atcgtattag aagccgtaca aaaggctgtt gaagacttta aagatctaga
    16801 aattatactt ttcggtgacg aaaaaaagta taatctgaac catgaacgaa tcgaatttag
    16861 acattgttct gaaaagattg aaatggaaga tgagcctgtt agagcgatta aacgtaaaaa
    16921 agatagctca atggtaaaaa tggctgaagc tgtgaaatct ggtgaagcag atggatgtgt
    16981 gtcagcaggt aatactggtg ctttaatgtc agctggttta ttcattgttg gacgtattaa
    17041 aggtgtagct agaccggctt tagtagtaac attgccaacg attgatggaa aaggttttgt
    17101 ctttttagac gttggtgcaa atgctgatgc taaacctgaa cacttattac agtatgcgca
    17161 actaggggat atttatgctc aaaaaattag aggtattgat aatccgaaaa tctcattatt
    17221 aaatatagga accgagccag ctaaaggtaa tagtttaacg aaaaaatcat atgagttatt
    17281 aaatcatgat cattcattga attttgttgg gaatattgaa gcgaagacat taatggatgg
    17341 cgatacagat gttgtagtta ccgatggcta tactgggaac atggtcctta aaaatttaga
    17401 aggtactgca aaatcaatcg gtaaaatgtt aaaagatacg attatgagta gtactaaaaa
    17461 taaattagca ggtgcaatat tgaagaaaga tttagctgaa ttcgctaaaa agatggatta
    17521 ctcagaatac ggtggttccg tattattagg attggaaggt actgtagtta aagcacacgg
    17581 tagttcaaat gctaaagctt tttattctgc aattagacaa gcgaaaatcg caggagaaca
    17641 aaatattgta caaacaatga aagagactgt aggtgaatca aatgagtaaa acagcaatta
    17701 tttttccggg acaaggtgcc caaaaagttg gtatggcgca agatttgttt aacaacaatg
    17761 atcaagcaac tgaaatttta acttcagcag cgaacacatt agactttgat attttagaga
    17821 caatgtttac tgatgaagaa ggtaaattgg gtgaaactga aaacacacaa ccagctttat
    17881 tgacgcatag ttcggcatta ttagcagcgc taaaaaattt gaatcctgat tttactatgg
    17941 ggcatagttt aggtgaatat tcaagtttag ttgcagctga cgtattatca tttgaagatg
    18001 cagttaaaat tgttagaaaa cgtggtcaat taatggcgca agcatttcct actggtgtag
    18061 gaagcatggc tgcagtattg ggattagatt ttgataaagt cgatgaaatt tgtaagtcat
    18121 tatcatctga tgacaaaata attgaaccag caaacattaa ttgcccaggt caaattgttg
    18181 tttcaggtca caaagcttta attgatgagc tagtagaaaa aggtaaatca ttaggtgcaa
    18241 aacgtgtcat gcctttagca gtatctggac cattccattc atcgctaatg aaagtgattg
    18301 aagaagattt ttcaagttac attaatcaat ttgaatggcg tgatgctaag tttcctgtag
    18361 ttcaaaatgt aaatgcgcaa ggtgaaactg acaaagaagt aattaaatct aatatggtca
    18421 agcaattata ttcaccagta caattcatta actcaacaga atggctaata gaccaaggtg
    18481 ttgatcattt tattgaaatt ggtcctggaa aagttttatc tggcttaatt aaaaaaataa
    18541 atagagatgt taagttaaca tcaattcaaa ctttagaaga tgtgaaagga tggaatgaaa
    18601 atgactaaga gtgctttagt aacaggtgca tcaagaggaa ttggacgtag tattgcgtta
    18661 caattagcag aagaaggata taatgtagca gtaaactatg caggcagcaa agagaaagct
    18721 gaagcagtag tcgaagaaat caaagctaaa ggtgttgaca gttttgcgat tcaagcaaat
    18781 gttgccgatg ctgatgaagt taaagcaatg attaaagaag tagttagcca atttggttct
    18841 ttagatgttt tagtaaataa tgcaggtatt actcgcgata atttattaat gcgtatgaaa
    18901 gaacaagagt gggatgatgt tattgacaca aacttaaaag gtgtatttaa ctgtatccaa
    18961 aaagcaacac cacaaatgtt aagacaacgt agtggtgcta tcatcaattt atcaagtgtt
    19021 gttggagcag taggtaatcc gggacaagca aactatgttg caacaaaagc aggtgttatt
    19081 ggtttaacta aatctgcggc gcgtgaatta gcatctcgtg gtatcactgt aaatgcagtt
    19141 gcacctggtt ttattgtttc tgatatgaca gatgctttaa gtgatgagct taaagaacaa
    19201 atgttgactc aaattccgtt agcacgtttt ggtcaagaca cagatattgc taatacagta
    19261 gcgttcttag catcagacaa agcaaaatat attacaggtc aaacaatcca tgtaaatggt
    19321 ggaatgtaca tgtaatatat ttgagctaaa gctcattgac gcagtggttg actggtcatc
    19381 caatggagaa ttgtctgacc tagtcaactt tgcgggggaa attctaagca acctagataa
    19441 ggttccagaa tttctcccta agaaacacta atcaataaat tgataagtgt ttctaaaatt
    19501 tctacttgtt ttttagaatt taaaatggga aaatatagta gtctatgtat aggcattttt
    19561 aaaggaggtg aatcgacgtg gaaaatttcg ataaagtaaa agatatcatc gttgaccgtt
    19621 taggtgtaga cgctgataaa gtaactgaag atgcatcttt caaagatgat ttaggcgctg
    19681 actcacttga tatcgctgaa ttagtaatgg aattagaaga cgagtttggt actgaaattc
    19741 ctgatgaaga agctgaaaaa atcaacactg ttggtgatgc tgttaaattt attaacagtc
    19801 ttgaaaaata ataaatctta catctgggtc gtcagtattg tcgactcagt ttttttcttt
    19861 aattatcaat agttttaacg taaaattaaa gatgattcaa gagcaacaca taaaggagat
    19921 aaaataatgt ctaaacaaaa gaaaagtgag atagttaatc gttttagaaa gcgctttgat
    19981 actaaaatga cagagttagg ctttacttat caaaatattg atttatacca acaagcattt
    20041 tcgcattcga gttttattaa tgattttaat atgaatcgtt tagaccataa tgagcgttta
    20101 gagtttttgg gtgatgcggt attagaattg acggtttcac gatatttatt tgataaacat
    20161 cccaacttgc cagaagggaa tttaacaaaa atgcgtgcca ctattgtatg tgagccctca
    20221 cttgtaatat ttgcgaataa aattggattg aacgaaatga ttttacttgg taaaggtgaa
    20281 gagaaaacag ggggacgtac aagaccatca ttaatatcag atgcattcga agcatttatt
    20341 ggggcattgt atttggatca aggactagat atagtttgga aatttgctga gaaagtcatt
    20401 ttcccacatg tagaacaaaa tgagttatta ggcgtggtag attttaaaac acaattccaa
    20461 gaatatgtgc accagcaaaa taaaggtgat gtaacctata atttaataaa agaagaggga
    20521 ccggcacatc atcgtctatt cacttcagaa gttattctgc aaggggaagc aatagctgaa
    20581 ggtaaaggga aaacgaaaaa agaatcagaa caacgtgctg ctgaaagtgc ctataagcaa
    20641 ttaaaacaaa ttaaatagaa atttatatga tacgacatat aggtgttcga tttaggattt
    20701 aaggaagaat ttttaatatt ttatggaaca acttcgaaat ttaatataca aatactaaaa
    20761 tatcgtatac atgtgttctt taaaattgtg ataaggagtt taggatggtt tatttaaaat
    20821 caatagatgc cattggattt aagtcttttg cagatcaaac caatgttcaa ttcgataaag
    20881 gtgtaactgc aattgttggt ccaaatggaa gcggtaaaag taatattaca gatgctatta
    20941 aatgggtgtt gggtgaacaa tcggctaaat cattacgtgg ctcaaaaatg gaagatatta
    21001 tcttctcagg tgcagaacat cgcaaagctc aaaattatgc tgaagtacag ttaagattag
    21061 ataatcattc taaaaagctc agtgttgatg aaaacgaagt tattgtaaca agaagattgt
    21121 atcgaagtgg tgaaagtgag tactacataa ataatgaccg tgcaagatta aaagatattg
    21181 ccgatttatt tttagattct ggattgggaa aagaagcgta tagcattatc tcgcaaggta
    21241 gagttgatga aatactaaat gctaaaccaa ttgatagacg tcaaattatt gaagaatcgg
    21301 ctggtgtact taaatataaa aaacgtaagg ctgaatcatt aaataaactt gaccaaacag
    21361 aagataattt aacgagagta gaagacattt tatatgattt ggaaggtcgc gtagaacctc
    21421 taaaagagga ggcagctata gctaaagaat ataagacact ttcacatcaa atgaaacata
    21481 gtgacattgt agttacagtg cacgatattg atcaatatac aaatgacaat agacaattag
    21541 atcaacgttt aaatgattta caaggccaac aagcaaataa agaagctgac aagcaacgtt
    21601 taagccaaca aattcaacaa tataaaggta aacgtcatca acttgataat gatgttgaat
    21661 cgcttaatta tcaattagta aaagctacgg aagcctttga aaaatatacg ggacaattaa
    21721 atgttttgga agaacgtaag aaaaatcaat ctgaaacaaa tgcacgatat gaagaagaac
    21781 aagaaaattt aatggagctt ttagaaaata tatcaaatga gatttctgaa gctcaagata
    21841 cttataagtc tctgaaaagt aaacaaaaag aactcaatgc tgtcattcgt gaacttgaag
    21901 aacaactata tgtttcagac gaagcacatg atgaaaaatt ggaagaaatt aaaaacgaat
    21961 actatacatt aatgtcagag caatcagatg ttaacaatga tattcgtttt ttaaagcata
    22021 ctatagaaga gaatgaggct aaaaaatcaa gactagattc tcgattagtt gaagtttttg
    22081 agcaattgaa agatattcag ggtcaaataa aaacgacaaa aaaagaatat caacagacca
    22141 acaaagaact ttctgctgta gataaagaaa ttaaaaatat agaaaaagac ctcactgata
    22201 caaaaaaagc acaaaatgaa tacgaagaga aattgtatca agcatatcga tataccgaaa
    22261 aaatgaaaac acgtattgat agtttggcaa cgcaagagga agaatatact tattttttca
    22321 atggcgtcaa acatattttg aaagctaaaa ataaagaatt aaagggtatt tatggtgcag
    22381 ttgcggaaat tattgatgtg ccatctaaat taactcaggc aattgaaaca gcattaggtg
    22441 cttcattaca acatgtcatt gtagattcag aaaaagatgg acgccaggct attcaatttt
    22501 taaaagaacg taatttaggt cgtgcgacgt ttttaccatt aaatgttata cagagtagag
    22561 tggtagcgac tgatattaaa tctattgcta aagaggcaaa cggatttatt agtatcgctt
    22621 cggaagcagt taaagtagca ccagaatatc aaaatattat cgggaattta ttaggtaata
    22681 cgattatcgt tgatcattta aagcatgcaa atgaattggc acgtgcgatt aaatatcgaa
    22741 ctcgtattgt tactttggaa ggtgatattg taaatcctgg tggttctatg actggtggtg
    22801 gcgctcgtaa gtcaaaaagt attctgtctc aaaaagacga gttgacaaca atgagacacc
    22861 aattagaaga ttacttgcgt caaacagaat catttgaaca acaatttaaa gagttgaaga
    22921 taaaaagtga tcaattaagt gaactgtatt ttgaaaaaag tcaaaagcat aatacactta
    22981 aagagcaagt gcatcatttt gaaatggagc tcgatagatt aactacacaa gaaacacaaa
    23041 taaaaaatga tcatgaagaa ttcgaatttg aaaaaaatga tggttatacg agtgacaaaa
    23101 gtcgacaaac tttgagtgaa aaagaaactt atctagaaag tattaaagca tctttaaaac
    23161 gactagaaga tgaaattgaa cgctacacaa aactttctaa agaaggtaag gaaagcgtta
    23221 ctaaaacaca acaaacctta catcagaaac aatctgatct tgctgtggtt aaagagcgta
    23281 ttaaaacaca acaacagaca atagatcgat taaataatca aaatcaacaa actaaacatc
    23341 aattaaaaga tgttaaagaa aaaattgcat tctttaattc ggatgaagtg atgggcgaac
    23401 aagcttttca aaatattaaa gatcaaatta atggtcaaca agaaacgaga acacgcttat
    23461 cagatgaatt agataaattg aaacaacaac gtattgagtt gaatgaacaa atcgatgcgc
    23521 aagaagctaa actacaagtt tgtcaccaag atattttagc tatcgaaaat cactaccaag
    23581 atattaaagc tgaacaatca aagctagatg tattaattca tcatgcgata gatcatttaa
    23641 atgatgaata tcaattgact gttgaacgtg cgaaatctga atatacgagt gatgaatcga
    23701 ttgacgcatt acgtaaaaaa gttaagttaa tgaagatgtc gattgatgaa ctaggtcctg
    23761 taaacttaaa tgcaattgaa caatttgaag agttaaatga acgttataca tttttaagtg
    23821 aacaacgtac agatcttcgt aaagctaaag aaacattaga gcaaattata agtgaaatgg
    23881 atcaagaggt tactgaaaga tttaaagaaa ctttccatgc tattcaagga cattttacag
    23941 ctgtgttcaa acaattgttt ggtggaggcg atgcagaatt gcaattaact gaagccgatt
    24001 atttaacagc tggtattgat attgtggtac aaccaccggg taaaaagttg caacatttat
    24061 cgttactgag tggtggtgag cgtgcattaa ctgctattgc tttactattt gcaattttaa
    24121 aagtaagatc tgcacctttt gttatattag atgaggttga agctgcacta gatgaagcaa
    24181 atgttattag atacgcaaaa tatttaaatg agttatcaga cgaaacacaa ttcattgtta
    24241 ttacacaccg taaaggaaca atggaatttg cagataggtt atacggtgta acaatgcaag
    24301 aatcaggtgt tactaaactt gtgagtgtga atttaaatac aatagatgat gtgttgaagg
    24361 aggagcaata atgagctttt ttaaacgctt aaaagataag tttgcaacaa ataaagaaaa
    24421 tgaagaagtt aaatccttaa cagaagaaca aggtcaagac aaattagaag atacacattc
    24481 tgaaggttca acgcaggacg caaatgattt agcagaaaat gctgaagtga aaaagaagcc
    24541 acgcaagttg agtgaagcgg attttgatga cgatggctta atatcaattg aagattttga
    24601 agaaattgaa gctcaaaaaa tgggtgctaa atttaaagca ggactcgaaa aatctcgtca
    24661 aaatttccaa gaacaattaa ataatttgat agcgagatat cgtaaagtag atgaagactt
    24721 ttttgaagct ttagaagaaa tgttaatcac tgcagacgtc ggttttaata cagtgatgac
    24781 gttaactgaa gaattacgta tggaagcaca acgacgtaat attcaagata ctgaagattt
    24841 gcgtgaagtc attgttgaaa aaatcgtaga gatttaccat caagaagatg ataattcaga
    24901 agctatgaac ttagaagatg gtcgtttaaa tgtcatttta atggttggtg tgaatggtgt
    24961 tggtaaaaca acaacaattg gaaaattagc ttaccgatat aaaatggaag gtaaaaaagt
    25021 aatgttagct gcgggcgata cttttagagc gggtgctatt gatcaattga aagtttgggg
    25081 cgaacgtgtt ggtgtagacg taattagcca aagtgaaggt tctgatccag ctgctgttat
    25141 gtatgatgcg attaatgccg ctaaaaacaa aggtgttgat attttaatct gtgataccgc
    25201 tggacgttta caaaataaaa caaatctaat gcaagaatta gaaaaagtta agcgtgtaat
    25261 taatcgagca gtgccagatg cgcctcatga agcattacta tgtttagatg ctacaactgg
    25321 tcagaatgcg ttgtcacaag ctagaaactt taaagaagta acaaatgtta caggtattgt
    25381 attaacgaaa ttagatggta cagccaaagg tggtatcgta ttagccattc gtaatgaatt
    25441 gcacatccca gttaaatatg taggtttagg tgagcaatta gatgacttac aaccatttaa
    25501 ccctgaaagt tatgtctacg gcttattcgc tgatatgatt gaacaaaatg aagaaataac
    25561 aacagttgaa aatgatcaaa ttgtaacaga agaaaaggac gataatcatg ggtcaaaatg
    25621 atttagttaa aacgttacga atgaattatt tgtttgattt ttatcaatcc ttattgacga
    25681 ataaacaacg taattatttg gaattatttt atcttgaaga ttattcttta agtgaaatcg
    25741 cagatacttt taatgtgagt agacaagcag tttatgataa tataagaaga actggcgatt
    25801 tagttgaaga ttatgaaaag aaattggaat tataccagaa atttgagcaa cgccgagaaa
    25861 tatatgatga aatgaaacaa catttaagta atccagaaca aatacaacgt tatattcaac
    25921 aattagaaga cttagaatag atataaattg agggaggaca tcgatatggc atttgaaggg
    25981 ttatcagaac gcttgcaagc gacgatgcaa aaaatgcgtg gtaagggtaa acttactgaa
    26041 gctgatataa agataatgat gcgtgaagta agattagcgt tacttgaggc tgacgtaaac
    26101 tttaaagtgg taaaagaatt tattaaaaca gtatcagaac gcgcattagg ttccgatgta
    26161 atgcaatcat taacaccagg gcaacaagtt attaaaatag ttcaagatga attaacgcag
    26221 ttgatgggtg gagaaaatac gtcgattaat atgtcaaata aaccacctac tgttgttatg
    26281 atggttggtt tacaaggtgc tggtaaaaca acaactgcag gtaaattagc attattgatg
    26341 cgtaaaaaat acaacaaaaa acctatgtta gttgcagcag atatttatcg tccagcagcg
    26401 ataaatcaat tacaaacagt agggaaacaa attgatattc ctgtatacag tgaaggagat
    26461 caagtaaagc cacaacaaat tgtaactaat gcattaaaac atgctaaaga agaacattta
    26521 gactttgtaa tcattgatac agcaggtcga ttacacatcg atgaagcatt gatgaacgaa
    26581 ttaaaagaag taaaagacat tgctaaacca aacgaaatta tgttagttgt cgattcaatg
    26641 acgggtcaag atgctgtcaa tgttgcagaa tcttttgacg atcaacttga tgtcacaggt
    26701 gttaccttaa ctaaattaga tggtgataca cgtggtggtg cagctttatc tattcgttcg
    26761 gtgacacaaa aaccaattaa atttgttggt atgagtgaaa agttagatgg tttagagcta
    26821 ttccatcctg aacgtatggc atcacgtatt ttaggtatgg gtgatgtgtt aagtttaatt
    26881 gaaaaagcgc aacaagatgt ggatcaagaa aaagcaaaag atttagagaa aaagatgcgt
    26941 gagtcatcgt ttactttaga tgatttttta gaacaacttg atcaggtgaa aaatctagga
    27001 ccactggatg atattatgaa aatgattcca ggtatgaata aaatgaaagg gctagataag
    27061 cttaatatga gtgaaaagca aattgatcat attaaagcga ttatccagtc aatgacgccg
    27121 gctgaaagaa acaatccaga cacattgaat gtatcacgta aaaagcgtat tgctaaaggg
    27181 tctggtcgtt cattacaaga agtcaatcgt ttgatgaaac aatttaacga tatgaagaaa
    27241 atgatgaaac agttcactgg tggcggtaaa ggtaaaaaag gtaaacgcaa tcaaatgcaa
    27301 aatatgttaa aaggtatgaa tttaccgttt taatgtttaa tataacacgc tgatttttgt
    27361 tgcaatgaga tgtgcatccc gaaagttata ctaaagaata taactttatg gggtgcatat
    27421 ttttagatac acaataataa ttaaaaactg aaattgctga tttgctagaa catgaggtta
    27481 ttgttgctaa tattaataag ttatattggg ggaacgtgtg cggtagaagt ggtaccattc
    27541 atgtccttac gagataaatt aacttaacac gtaggtggtg aaatttacta tgaattttta
    27601 taacctatta acagtctaat ttagtaatca catagttatt attgtaagaa aaaggttcaa
    27661 taattttcct ggtaaagaaa aaactcttta caaacattca tacacctgtt aatattattt
    27721 cttgtagaaa ataaaaatta aaacatgact taaaggagat tttataaatg gcagttaaaa
    27781 ttcgtttaac acgtttaggt tcaaaaagaa atccattcta tcgtatcgta gtagcagatg
    27841 ctcgttctcc acgtgacgga cgtatcatcg aacaaatcgg tacttataac ccaacgagcg
    27901 ctaatgctcc agaaattaaa gttgacgaag cgttagcttt aaaatggtta aatgatggtg
    27961 cgaaaccaac tgatacagtt cacaatatct tatcaaaaga aggtattatg aaaaaatttg
    28021 acgaacaaaa gaaagctaag taatttagcg taaaattgtt ctaacaataa gaataactcg
    28081 tttacactga cagttattac tcaatgatac gttgggaata tcacatgtta gtaatataga
    28141 acgtattggg taccataatg gtgccctttt tctttgaatt attttcaatt aaaatagaag
    28201 tgttcaaagc atagagttgg aggtaataga atgagagttg aagttggtca aattgttaac
    28261 acacatggta ttaaaggtga aattaaagta aaatccaatt cagactttac agacgttcgt
    28321 tttcaacccg gtcaagtgct gacagttgtg cataacaata acgaccttga atatactgtt
    28381 aagtcacata gagtgcataa agggcttcat atgcttacat ttgaaggtat taataatatt
    28441 aatgatattg agcatttaaa agggagttct atttatcaag agcgtgatca tgaagatatc
    28501 gtacttgagg aaaatgaatt ttattattca gatattatag gatgtacagt ttttgatgat
    28561 caagaaacac caataggtcg tgtaattaat atatttgaaa caggtgcgaa tgatgtgtgg
    28621 gtgattaaag gatctaaaga atatttgatt ccttatattg ctgatgttgt aaaagaagtg
    28681 gatgttgaaa ataaaaaaat tatcatcacg ccaatggaag gattgttgga ttaatgaaaa
    28741 ttgattattt aactttattt cctgaaatgt ttgatggtgt tttaaatcat tcaattatga
    28801 aacgtgccca agaaaacaat aaattacaaa tcaatacggt taattttaga gattatgcaa
    28861 ttaacaagca caaccaagta gatgattatc cgtatggtgg cggacaaggt atggtgttaa
    28921 agcctgaacc tgtttttaat gcgatggaag acttagatgt cacagaacaa acacgcgtta
    28981 ttttaatgtg tccacaaggc gagccatttt cacatcagaa agctgttgaa ttaagcaagg
    29041 ccgaccacat cgttttcata tgcggacatt atgaaggtta cgatgaacgt atccgaacac
    29101 atcttgtcac agatgaaata tcaatgggtg actatgtttt aactggtgga gaattgccag
    29161 cgatgaccat gactgatgct attgttagac tgattccagg tgttttaggt aatgaacagt
    29221 cacatcaaga cgattcattt tcagatgggt tattagagtt tccgcaatat acacgtccgc
    29281 gtgaatttaa gggtctaaca gttccagatg ttttattgtc tggaaatcat gccaatattg
    29341 atgcatggag acatgagcaa aagttgatcc gcacatataa taaaagacct gacttaattg
    29401 aaaaatatcc attaactaat gcagataagc aaatattaga aagatataaa ataggattga
    29461 aaaaaggtta gtattgtgct aacataatct gagtgtggaa acactaaaat cactatgatc
    29521 cgctgctata tatttgtcga ggcaagaaca taggttgaag aggagaattt ataatgacaa
    29581 atcacaaatt aatcgaagca gtaactaaat cacaattacg tacagactta ccaagtttcc
    29641 gtcctggtga tactttacgt gtacacgtac gtatcattga gggtactcgt gagcgtatcc
    29701 aagtattcga aggcgttgta attaaacgtc gtggcggtgg cgtttctgaa acgtttacag
    29761 ttcgtaaaat ttcatcaggt gttggcgtgg aacgtacatt cccattacac acaccaaaaa
    29821 ttgaaaaaat cgaagttaaa cgtcgtggta aagtacgtcg tgctaaatta tattacttac
    29881 gtagtttacg tggtaaagct gctagaatcc aagaaattcg ttaatcagca tttaaacaaa
    29941 gctatgtatg agtcaaattc gactcaaaca ataaagccat ctaggtcact tttatggagt
    30001 gatatagatg gctttttttg ctgtttagtt aatgtaaagt tgataatgta attgtttatt
    30061 ccttctctat tcataacgtg tttgctcaaa atggttatat aaacaaaaat ctactaagtg
    30121 ccattgaaaa ggacttagta gatttattaa atatatgcgt agcaacatta tttttgtctt
    30181 gcccatctcg tgaaaataat actacatata atgccaaata tagtaattgt tataagtaaa
    30241 taatagtatg gtggggtata gctcaattga atctttgtta tatttttagg tgctttaatg
    30301 ccggtcataa caccatttac ttgttcaact ttaagacttt gatcacctga tgtcgctttc
    30361 ataccttgat tatatgctgt tggcaaaaca atatacccag atgaattttt atttttagta
    30421 atagtataac catgctttgt cttactaact ttgacagctt ctaatgaatt tgaagcgtct
    30481 ttaagcgtgg tataatcttc gccgtatatc ccttttaaat ttactcgata cttaccttta
    30541 ggcaatgata atctaattct atctggagct ttaatgcgta tcgttacggg tgttacaacg
    30601 cgtcgatatt tataagtgag tttatttctt tcttgtgtat attcattcac tttaacatca
    30661 tgagctttat ccggcgaaag taattctaaa tccatttcaa aatacaaatc tttaaattga
    30721 ttagaaactg attttggcaa ctgtacagtt agaccaccat tattttgttt aacttgtaat
    30781 aaatgttttg taggagattg ccaggctgca ctatttaatt taattgttga atctgatagt
    30841 aaatttttat tggctttaaa atgtgtatta acatctttaa tattgttaga aacaatccct
    30901 tgcaacattg cttgttcttt atctaatgga gattttaatt ctttattgga aaagacctta
    30961 tttgtaatat gtgcacttgg ataatggatg gtatttttag aatgaatcca acgaacttta
    31021 ttgtctttgt gttcagactt aattttaaat ccatatggta agttgtcatc atgattcact
    31081 ctaattcgat cattaacatt ccaaagtgat agtaaatttt gacgattgcc aagtaatcta
    31141 taagtgctgt ttttatcgat tggcatatta atttggagtg tcttgtcata atattttaaa
    31201 atgtctccat taaaaatact agaatataat gaaatgccat tataatgata tataaatggt
    31261 gaatttaatg catagtctga catataatca atgcgattaa atgagcctgt tgcattttga
    31321 tttatctttt ttataagctg gtttacatag ttactatggt aatcatgttg tttcaacgtt
    31381 gataatgatt gttgataagg tttgattgcc atgtttttgt tgttatctaa aatgacgatt
    31441 tgttgaatca taacgattaa tactaatatt gcaacggtta attttttata acgccataaa
    31501 ctaaatttta aaataacggc aagcactatt agcaggataa tacctactat aagtgcaagt
    31561 gggtgtgtcg gtgatagtaa tacataaagt attgcgatga tgcatacggg tattgttctg
    31621 attaaataat atttcatatt taatgttgat aaatgttgaa taaacaatcc gcaaagagca
    31681 cttgatgata gtgctaagat atacacccaa cgcctttctg gaaatgaaaa accattaaaa
    31741 gcactgtcga aatactgtga taatgaacct ataaataata tccatgttac tattgcgaaa
    31801 agtctataaa agtaaaaacg atacagtttg aatgacaata atgcaacgat agtaagaatt
    31861 gaaatcgtaa tataaaatcc atcgctaaag aaaaaataat gataatcaag tggtgtcaaa
    31921 aacggtatat caacattggg attttgcttt ctgtcatttt ccaaaaacgc agaaatgcca
    31981 gtgaataaac caaatacact tgataacaca ctcaaaactg tagcagatat gacgcaaatt
    32041 aatttttgtg ttctagagac aatgtcatat ttgtaagtga aaatgagtcg atataaatag
    32101 tagcaaccta taataatagc ttgataataa ctgaaataaa aattgctaaa tagtgttaag
    32161 gctatcgcaa caatgaaaat accgattttg cgttgttgaa aatatctttc caaaccaaga
    32221 atcgataatg gcaataaata taataaattt ccataaaatg accaagtaaa attaaagtat
    32281 ataacgacag ttgacatgcc gtataaaatc gtagcgatca tatttgctga gcgtttaaag
    32341 tgtaatattt taaataagta gaaggtcacg acaaatgtta tgatagctcg tatcatagcc
    32401 ataataagtt ggtttgtcgg ccaaaaatgt attgtcgtcg gattaaatat accaaccgtt
    32461 tctcctattt taatgaatag aaaatttagc cacattaaag gtgacagcga ataataatat
    32521 gatagtcctt tcatataatc gccacctaat ccaaacgatg catcatataa actagaaaaa
    32581 ctacgtagat gttcatacaa atacatttga aatggcatca tttgacggaa tccatctcca
    32641 gccccgctaa aaacagtacc attcacaata taatcataga tatgagtaga aaataaaata
    32701 agcgttaata ttacactaat gaaagttata acaaagaatt gtttgacgtt tgaatttagc
    32761 cactttttta acacaacatt atcctcaact ttcaaattta aaattaagtt taactgaaac
    32821 taaagttaat gaggttcttg ataggtaaag acgaagatga ctgtggaaca gataccttat
    32881 catagttact taaactttgg atcattttca gtttatcatt aaacaaatat attgaataat
    32941 aaaaatgtca tactgataaa gatgaatgtc acttaataag taacttagat ttaacaaatg
    33001 atgattttta attgtagaaa acttgaaata atcacttata cctaaatcta aagcattgtt
    33061 aagaagtgtg acaatgttaa aataaatata gttgaattaa tgaatttgtt ctataattaa
    33121 caggttatag attttaataa tgagaaaaga attgacgaaa gtaaggtgaa ttgaatggtt
    33181 attcaatggt atccaggaca tatggcgaaa gccaaaagag aagtaagtga acaattaaaa
    33241 aaagtagatg tagtgtttga actagtagat gcaagaattc catatagttc aagaaaccct
    33301 atgatagatg aagttattaa ccaaaaacca cgtgttgtta tattaaataa aaaagatatg
    33361 tctaatttaa atgagatgtc aaaatgggaa caatttttta ttgataaagg atactatcct
    33421 gtatcagtgg atgctaagca cggtaaaaat ttaaagaaag tggaagctgc agcaattaag
    33481 gcgactgctg aaaaatttga acgcgaaaaa gcgaaaggac ttaaacctag agcgataaga
    33541 gcaatgatcg ttggaattcc aaatgttggt aaatccacat taataaataa actggcaaag
    33601 cgtagtattg cgcagactgg taataaacca ggtgtgacca aacaacaaca atggattaaa
    33661 gttggtaatg cattacaact attagacaca ccagggatac tttggcctaa atttgaagat
    33721 gaagaagtcg gtaagaagtt gagtttaact ggtgcgataa aagatagtat tgtgcactta
    33781 gatgaagttg ccatctatgg attaaacttt ttaattcaaa atgatttagc gcgattaaag
    33841 tcacattata atattgaagt tcctgaagat gcagaaatca tagcgtggtt tgatgcgata
    33901 gggaaaaaac gtggcttaat tcgacgtggt aatgaaattg attacgaagc agtcattgaa
    33961 ctgattattt atgatattcg aaatgctaaa ataggaaatt attgttttga tatttttaaa
    34021 gatatgactg aggaattagc aaatgacgct aacaattaaa gaagttacgc agttgattaa
    34081 tgcggttaat acaatagaag aattagaaaa tcatgaatgc tttttagatg agcgaaaagg
    34141 tgttcaaaat gccatagcta ggcgcagaaa agcgttagaa aaagaacaag ctttaaaaga
    34201 aaagtatgtt gaaatgactt actttgaaaa tgaaatatta aaagagcatc ctaatgctat
    34261 tatttgtggg attgatgaag ttggaagagg acctttagca ggtccagtcg ttgcatgcgc
    34321 aacaatttta aattcaaatc acaattattt gggccttgat gactcgaaaa aagtacctgt
    34381 tacgaaacgt ctagaattaa atgaagcact aaaaaatgaa gttactgctt ttgcatatgg
    34441 gatcgcgaca gctgaagaaa tagatgaatt taatatttat aaagctactc aaatcgccat
    34501 gcagcgagct attgatggat tatcagtaca accaacgcat ttattgatag acgcgatgac
    34561 gcttgataat gcactgcctc aagtatcttt aatcaagggt gatgcaagaa gtgtgtccat
    34621 tgcagctgca agtatcatgg caaaggtttt tcgtgatgat tatatgacac agttatctaa
    34681 agattatcct gaatatggtt ttgaaaaaaa cgcgggttac ggtaccaaac aacatttact
    34741 agcaatcgat gatattggca ttatgaaaga gcatagaaaa agctttgaac ctataaaatc
    34801 gttactgtaa atcgtaaaat tcaattaaat gataaaaaag tagcatttaa gtttacaata
    34861 gcgcttacat tttttataat tgtaagggaa cttaacctaa gtaacaggag gatggaagat
    34921 gaatatccac gagtatcaag gtaaagaaat atttcgttca atgggcgttg cagttccaga
    34981 aggacgagta gcatttactg ctgaagaagc ggtggagaaa gcaaaagaat taaattctga
    35041 tgtttatgtt gtaaaagcac aaattcatgc tggaggtaga ggtaaagcag gcggagtaaa
    35101 aattgctaaa tctttatctg aggtagaaac atatgcaaaa gaattattag ggaaaacttt
    35161 ggtgacacat caaactggtc cagaaggtaa agaaattaag cgtttatata tcgaagaagg
    35221 ttgtgctatt caaaaagaat attacgttgg attcgttatt gatcgtgcga ctgaccaagt
    35281 aacattgatg gcgtctgaag aagggggcac tgagattgaa gaagttgctg cgaagactcc
    35341 tgaaaagatc ttcaaagaaa ctatcgatcc agtaatcgga ctttcaccat tccaagcaag
    35401 acgaattgcg tttaatatta atattcctaa agaatctgtt aacaaagctg ctaaattctt
    35461 attagcactt tataatgtat tcattgaaaa agattgttca atcgtagaaa tcaacccatt
    35521 agttacaaca gctgatggtg atgtattggc attagatgct aaaattaatt ttgatgataa
    35581 tgcattattc agacataaag atgttgtaga attacgtgat ttagaagaag aagatccgaa
    35641 agagattgaa gcgtctaaac atgatttatc atacattgca ttagatggtg acatcggatg
    35701 tatggttaat ggtgcaggtt tagccatggc aacaatggat acgattaatc atttcggtgg
    35761 aaacccagcc aatttcttag atgcaggcgg aagcgctact agagaaaaag taactgaagc
    35821 atttaaaatc attttaggtg atgaaaatgt taaaggtatt tttgtaaaca ttttcggtgg
    35881 cattatgaaa tgtgatgtta tcgcagaagg tatcgttgaa gctgtaaaag aagtagattt
    35941 aactttacca ctagttgtac gcctagaagg tacaaatgtt gagttaggta aaaaaatctt
    36001 aaaagactca ggattagcaa ttgaaccagc agcaacaatg gctgaaggtg cacaaaaaat
    36061 tgttaaacta gtcaaagaag cataaggaaa ggatgggagc actaagatga gtgtatttat
    36121 agataagaat actaaagtaa tggtacaagg tattacaggg tctactgccc ttttccatac
    36181 aaaacaaatg cttgattatg gtacgaaaat agtagcaggt gtgacgcctg gtaaaggtgg
    36241 tcaagttgtt gaaggcgttc ctgttttcaa cactgttgaa gaagctaaaa atgaaactgg
    36301 ggcaacggtt tcagtcattt acgttccagc accatttgct gcagactcaa ttttagaagc
    36361 agctgatgca gacttagata tggttatttg tatcactgaa catattcctg tattagacat
    36421 ggttaaagtt aaacgctact tacaaggtag aaaaacacgt ttagttggtc cgaactgtcc
    36481 aggtgtgatt acagcagatg aatgtaaaat tggtattatg cctggctata ttcacaaaaa
    36541 aggtcatgtt ggtgtagtat ctcgttcagg tacattaaca tatgaagcag tgcaccaatt
    36601 gactgaagaa ggtattggtc aaactacagc tgttggtatt ggtggagacc cagtcaacgg
    36661 aacaaacttt attgatgttt taaaagcatt caatgaagat gacgaaacga aagcagttgt
    36721 tatgattggt gaaatcggtg gtacggctga agaagaagca gctgaatgga ttaaagcgaa
    36781 tatgacaaaa ccagttgtag gctttatcgg tggacaaaca gcacctcctg gaaaacgtat
    36841 gggacatgct ggtgcaatca tttcaggtgg taaaggtact gctgaagaga aaattaaaac
    36901 attaaatagt tgtggtgtga aaacagcggc aacaccttca gaaattggtt caacattaat
    36961 tgaagctgct aaagaagcag gtatttatga atcattatta actgttaata aataaagtta
    37021 aaagatgata taaatggcta tagcccattc catactttat aaaagtatcg gaaatgggct
    37081 atttgtcgtg cgatgaattt tagtacactt ttcatatttt ggaaagacaa gttaattatt
    37141 ttacctccta caatagcttt ataaaacaaa gaaaatttta attaaaatat atagcgcact
    37201 atgttataat gtttgtatat tattgtaagg agtgtttcat catgaataaa caacaaagta
    37261 aagtacgcta ttcaattaga aaagttagta ttggaatttt gtcaatttca ataggtatgt
    37321 ttttggcatt gggtatgtcg aacaaagcat atgcagatga aattgataaa tctaaagatt
    37381 ttacaagagg gtatgagcaa aatgtattcg cgaaatcaga gttaaatgct aataaaaata
    37441 cgacaaaaga caaaataaaa aatgaaggtg ctgttaaaac atcggacaca agtttaaagt
    37501 tagacaacaa atcagcaatt tcaaacggaa atgaaattaa tcaagatata aagatttcaa
    37561 atactccgaa aaactcaagc caaggtaaca atctagttat taataacaat gaacttacta
    37621 aagaaattaa aattgcaaac ttggaagctc aaaattctaa tcagaagaaa acgaataaag
    37681 ttactaataa ttactttggt tactacagtt ttagagaagc tccaaaaaca caaatctata
    37741 ctgtaaaaaa aggagacaca cttagtgcta tagcattaaa atacaaaact acagtttcaa
    37801 atattcaaaa tacaaataat atagcaaatc ctaatttaat atttatt
//
LOCUS       DAKTIO010000007        37549 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.007, whole genome shotgun sequence.
ACCESSION   DAKTIO010000007 DAKTIO010000000
VERSION     DAKTIO010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 37549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37549
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.007"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            134..1801
                     /locus_tag="O0293_000242"
     CDS             134..1801
                     /locus_tag="O0293_000242"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HCY0279155.1"
                     /translation="MTHLSDLDIANQSTLQPIKDIAASVGISEDALEPYGHYKAKIDI
                     NKITPRENKGKVVLVTAMSPTPAGEGKSTVTVGLADAFHELNKNVMVALREPALGPTF
                     GIKGGATGGGYAQVLPMEDINLHFNGDFHAITTANNALSAFIDNHIHQGNELGIDQRR
                     IEWKRVLDMNDRALRHVNVGLGGPTNGVPREDGFNITVASEIMAILCLSRSIKDLKDK
                     ISRITIGYTRDRKPVTVADLKVEGALAMILKDAIKPNLVQSIEGTPALVHGGPFANIA
                     HGCNSILATETARDLADIVVTEAGFGSDLGAEKFMDIKVREAGFDPAAVVVVATIRAL
                     KMHGGVAKDNLKEENVEAVKAGIVNLERHVNNIKKFGVEPVVAINAFIHDTDAEVEYV
                     KSWAKENNVRIALTEVWKKGGKGGVDLANEVLEVIDQPNSFKPLYELELPLEQKIEKI
                     VTEIYGGSKVTFSSKAQKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEIT
                     IRELEAKTGAGFIVALTGAIMTMPGLPKKPAALNMDVTDDGHAIGLF"
     gene            2097..4784
                     /gene="harA"
                     /locus_tag="O0293_000243"
     CDS             2097..4784
                     /gene="harA"
                     /locus_tag="O0293_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haptoglobin-binding heme uptake protein HarA"
                     /protein_id="HCY0279156.1"
                     /translation="MNKHHPKLRSFYSIRKSTLGVASVIVSTLFLITSQHQAQAAENT
                     NTSDKISENQNNNATTTQPPKDTNQTQPATQPANTAKNYPAADESLKDAIKDPALENK
                     EHDIGPREQVNFQLLDKNNETQYYHFFSIKDPADVYYTKKKAEVELDINTASTWKKFE
                     VYENNQKLPVRLVSYSPVPEDHAYIRFPVSDGTQELKIVSSTQIDDGEETNYDYTKLV
                     FAKPIYNDPSLVKSDTNDAVVTNDQSSSVASNQTNTNTSNQNTSTINNANNQPQATTN
                     MSQPAQPKSSTNADQASSQPAHETNSNGNTNDKTNESSNQSDVNQQYPPADESLQDAI
                     KNPAIIDKEHTADNWRPIDFQMKNDKGERQFYHYASTVEPATVIFTKTGPIIELGLKT
                     ASTWKKFEVYEGDKKLPVELVSYDSDKDYAYIRFPVSNGTREVKIVSSIEYGENIHED
                     YDYTLMVFAQPITNNPDDYVDEETYNLQKLLAPYHKAKTLERQVYELEKLQEKLPEKY
                     KAEYKKKLDQTRVELADQVKSAVTEFENVTPTNDQLTDLQEAHFVVFESEENSESVMD
                     GFVEHPFYTATLNGQKYVVMKTKDDSYWKDLIVEGKRVTTVSKDPKNNSRTLIFPYIP
                     DKAVYNAIVKVVVANIGYEGQYHVRIINQDINTKDDDTSQNNTSEPLNVQTGQEGKVA
                     DTDVAENSSTATNPKDASDKADVIEPESDVVKDADNNIDKDVQHDVDHLSDMSDNNHF
                     DKYDLKEMDTQIAKDTDRNVDKDADNSVGMSSNVDTDKDSNKNKDKVIQLNHIADKNN
                     HTGKAAKLDVVKQNYNNTDKVTDKKTTEHLPSDIHKTVDKTVKTKEKAGTPSKENKLS
                     QSKMLPKTGETTSSQSWWGLYALLGMLALFIPKFRKESK"
     gene            4915..5007
                     /locus_tag="O0293_000244"
     CDS             4915..5007
                     /locus_tag="O0293_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279157.1"
                     /translation="MKIILLLFLIFGFIVVVTLKSEHQLTLFSI"
     gene            complement(4987..5892)
                     /locus_tag="O0293_000245"
     CDS             complement(4987..5892)
                     /locus_tag="O0293_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCY0279158.1"
                     /translation="MTNQDNNHQLNHRIYHFEKIYKAIKHVIVFIFMIFIAIVAIAVI
                     AMSLYFHHLTKTSDSLSDDALIKKVRQIPGDELLDHNNKNLLYEYNHSQNSLIIGPKT
                     SSPNVIKALTSSEDTLFYKHDGILPKAILRAMIQDIFNTDQSSGGSTITQQLVKNQVL
                     TNEKTYSRKANELRLAIRLEHLLSKDEIIYTYLNIVPFGRDYNGANISGIASASYSLF
                     GIPPKDLSIAQSAYLIGLLQSPYGYTPYEKDGTLKSDKDLKYSIQRQHYVLKRMLIED
                     QITEKEYNDALKYDIKSHLLNRKKR"
     gene            6274..7536
                     /locus_tag="O0293_000246"
     CDS             6274..7536
                     /locus_tag="O0293_000246"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCY0279159.1"
                     /translation="MTNVLIEDLKWRGLIYQQTDEQGIEDLLNKEQVTLYCGADPTAD
                     SLHIGHLLPFLTLRRFQEHGHRPIVLIGGGTGMIGDPSGKSEERVLQTEEQVDKNIEG
                     ISKQMHNIFEFGTDHGAVLVNNRDWLGQISLISFLRDYGKHVGVNYMLGKDSIQSRLE
                     HGISYTEFTYTILQAIDFGHLNRELNCKIQVGGSDQWGNITSGIELMRRMYGQTDAYG
                     LTIPLVTKSDGKKFGKSESGAVWLDAEKTSPYEFYQFWINQSDEDVIKFLKYFTFLGK
                     EEIDRLEQSKNEAPHLREAQKTLAEEVTKFIHGEDALNDAIRISQALFSGDLKSLSAK
                     ELKDGFKDVPQVTLSNDTTNIVEVLIETGISPSKRQAREDVNNGAIYINGERQQDVNY
                     ALAPEDKIDGEFTIIRRGKKKYFMVNYQ"
     gene            complement(7630..8904)
                     /locus_tag="O0293_000247"
     CDS             complement(7630..8904)
                     /locus_tag="O0293_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCY0279160.1"
                     /translation="MSDFNHTDHSTTNHSQTPRYRRPKFPWFKTVIVALIAGIIGALL
                     VLGIGKVLNSTILNKDGSTVQTTNNKGGNQLDGQSKKFGTVHEMIKSVSPTIVGVINM
                     QKASSVDDLLKGKSSKPSEAGVGSGVIYQINNNSAYIVTNNHVIDGANEIRVQLHNKK
                     QVKAKLVGKDAVTDIAVLKIENTKGIKAIQFANSSKVQTGDSVFAMGNPLGLQFANSV
                     TSGIISASERTIDAETTGGNTKVSVLQTDAAINPGNSGGALVDINGNLVGINSMKIAA
                     TQVEGIGFAIPSNEVKVTIEQLVKHGKIDRPSIGIGLINLKDIPEEEREQLHTDREDG
                     IYVAKADSDIDLKKGDIITEIDGKKIKDDVDLRSYLYENKKPGESVTVTVIRDGKTKE
                     VKVKLKQQKEQPKRQSRSERQSPGQGDRDFFR"
     gene            9076..9693
                     /locus_tag="O0293_000248"
     CDS             9076..9693
                     /locus_tag="O0293_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCY0279161.1"
                     /translation="MYSVISKILNFILVKMSKSLYVIGKDNIPKDSKYVVTCTHESYN
                     EVIMLGMALYPNQIHYMAKKELFKNKWIGKFLTSLNAFPVDRENPGPSTLKRPINLLK
                     DNKTVGIFPTGSRTSQEGAPLKRGASTIAMLSKSPILPVAYVGPTKIHGLLTGQAYIN
                     IGKPIDINDLPKDLKRNERIDYITKEIETRTAKLQQELHEIVKSL"
     gene            9821..11287
                     /locus_tag="O0293_000249"
     CDS             9821..11287
                     /locus_tag="O0293_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIBC"
                     /protein_id="HCY0279162.1"
                     /translation="MYKFFQNLGRSLMLPVAILPAAAIIAGIGNTLNALHATPKIAMF
                     FTTVGTTILEQLGILFAIGVAIGMAKKNDGVVALAATLGYFLVTVVLSPMKLAPLLGM
                     KASEINSAFEKMNNGNVFVGIVIGLIAAYAYNKFSETELPLALSFFSGKRLVPIMTAF
                     YCTFLVVILLFLWPLLYSWIVKFGESIVGLGSFGAFIYGVANRLLIPTGLHHALNSVF
                     WFDTIGINDIGKFQSGKDAIKGITGRYQAGFFPIMMFGIPAAALAMYHTAKTTQKKQV
                     YGWFLASSVAAFFVGVTEPIEFAFMFVAPILYVVHALLTGLSLFIAATFHWTAGFSFS
                     AGLIDYVLSLINPVSNHPLMLLVQGVVFFILYYVIFRVVIQVFNLNTIGRGENELVDP
                     TVVKDNIAPGENDIKQSKYHQHAIQILEGLGGQENIVNLTNCATRLRLELKDTSIIDK
                     QKIKNAGAVGVTVNGKHSTQVIVGTHVQQVADEIEKHL"
     gene            11394..12524
                     /locus_tag="O0293_000250"
     CDS             11394..12524
                     /locus_tag="O0293_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCY0279163.1"
                     /translation="MKKILFDVDGVFLSEERCFDVSALTVYELLMDKCYLGLHSHIDW
                     ETLTDNDIQDIRNRIFQKDKILNKLKSLGLNSNWDMLFIVFSIHLIDILKKLSHDEIE
                     AFMYQDEPVELKLQNISTNLADCFNLNEQLPLQFLDNVKVGKNNIYAALEEFATTELH
                     VSDATLFSLKGALWTLAQEVYQEWYLGSKLYEDVEKKIARTTFKTGYIYQEIILRPVD
                     EVKVLLNDLKGAGFELGIATGRPYTETVVPFENLGLLPYFEADFIATASDVLEAENMY
                     PQARPLGKPNPFSYIAALYGNNRDKYESYINKQDNIVNKDDVFIVGDSLADLLSAQKI
                     GATFIGTLTGLKGKDAAGELEAHHADYVINHLGELRGVLDNL"
     gene            complement(12485..12580)
                     /locus_tag="O0293_000251"
     CDS             complement(12485..12580)
                     /locus_tag="O0293_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279164.1"
                     /translation="MKPLVQSFTTSYAVKQQSNYKLSSTPLSSPK"
     gene            complement(12674..14278)
                     /locus_tag="O0293_000252"
     CDS             complement(12674..14278)
                     /locus_tag="O0293_000252"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCY0279165.1"
                     /translation="MKQFNVLVADPISKDGIKALLDHEQFNVDIQTGLSEEALIKIIP
                     SYHALIVRSQTTVTENIINAADSLKVIARAGVGVDNINIDAATLKGILVINAPDGNTI
                     SATEHTLAMLLSMARNIPQAHQSLTNKEWNRNAFKGTELYHKTLGVIGAGRIGLGVAK
                     RAQSFGMKILAFDPYLTDEKAKSLSITKATVDEIAQHSDFVTLHTPLTPKTKGLINAV
                     FFAKAKPSLQIINVARGGIIDEKALIKALDEGQISRAAIDVFEHEPATDSPLVAHDKI
                     IVTPHLGASTVEAQEKVAISVSNEIIEILIDGTVTHAVNAPKMDLSNIDDTVKSFINL
                     SQTVGELAIQLMYNAPSSIKITYGGDLASIDSSLLTRTIITHILKDDLGPEVNIINAL
                     MLLNQQQVTLNIENNKAETGFSNYLEVELSNDSDSVKVGASVFTGFGPRIVRINNFSV
                     DLKPNQYQIVSYHNDTPGMVGKTGALLGKYNINIASMTLGRTEAGGDALMILSVDQPV
                     SNNIIDELKQVGEYNQIFTTELTVQS"
     gene            complement(14265..15425)
                     /locus_tag="O0293_000253"
     CDS             complement(14265..15425)
                     /locus_tag="O0293_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HCY0279166.1"
                     /translation="MYYHQPLLLTPGPTPVPDAIMREIQAPMVGHRSKDFEDIAQQAF
                     QGLKPIFGSQNDVLILTSSGTSVLEASMLNIVNPEDHFVVIVSGAFGNRFKQIAQTYY
                     KNVHIYDVTWGEAVDVKDFINFLSTLNVEVKAVFSQYCETSTTVLHPIHELGNAINQF
                     NSNIYFVVDGVSCIGAVDVDINKDKIDVLVSGSQKAIMLPPGLAFVAYSHRAKEHFKE
                     VTTPKFYLDLNKYISSQADNSTPFTPNVSLFRGVNAYVETVKAEGFNHVIARHYAIRN
                     ALRSALKALDLTLLVNDKDASPTVTAFKPNTNDEVKIIKDELKNRFKITIAGGQGHLK
                     GQILRIGHMGKISPFDILSVVSALEIILTEHRKVNYIGKGISKYMEVIHEAI"
     gene            complement(15539..15985)
                     /locus_tag="O0293_000254"
     CDS             complement(15539..15985)
                     /locus_tag="O0293_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCY0279167.1"
                     /translation="MHQHDFKVQTSWQGGRNNVGNVQGDILSENISIPASLGGVGIGT
                     NPDEMLVSAASSCYIISLAATLERAKFTDISIEQQSIGTACLNNGKFSMSKIVHHPQI
                     QIPSDQIAQLEKRLPKLITIADNNCMISNAVRNNVDIKIYPIIQAK"
     gene            16065..16808
                     /locus_tag="O0293_000255"
     CDS             16065..16808
                     /locus_tag="O0293_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCY0279168.1"
                     /translation="MTLNKLKDELQIVSHRGLPSDFPENTMVGYREVMGLNVAMLEID
                     VHLTKDQHFVVIHDETIDRTSDGKGRIADYTLSQLKSFDFGSYKDVAFKGERIPTLDE
                     VLSLCLKYDKKLLIELKSPNLYPEIECKLLAFLEEKKVDATQVVIQSFDIECIEKLNT
                     LGSIYELGVLCSKRNYWYKKPNFSRIAQIASYVNPNYALVTRKFVDKAHHHQLQVMPY
                     TVNKLKTGEKLRQMGVDGLITDNPKLFIK"
     gene            complement(17024..17626)
                     /gene="rpsD"
                     /locus_tag="O0293_000256"
     CDS             complement(17024..17626)
                     /gene="rpsD"
                     /locus_tag="O0293_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCY0279169.1"
                     /translation="MARFRGSNWKKSRRLGISLSGTGKELEKRPYAPGQHGPNQRKKL
                     SEYGLQLREKQKLRYLYGMTERQFRNTFDIAGKKFGVHGENFMILLASRLDAVVYSLG
                     LARTRRQARQLVNHGHILVDGKRVDIPSYSVKPGQTISVREKSQKLNIIVESVEINNF
                     VPEYLNFDADSLTGTFVRLPERSELPAEINEQLIVEYYSR"
     gene            complement(17870..18334)
                     /locus_tag="O0293_000257"
     CDS             complement(17870..18334)
                     /locus_tag="O0293_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HCY0279170.1"
                     /translation="MTTINPTNYTLLKKQAASLIEDEHHMIAILSNMSALLNDNLDQI
                     NWVGFYLLEQNELILGPFQGHPACVHIPIGKGVCGTAVSERRTQIVADVHQFEGHIAC
                     DANSKSEIVVPIFKDDKIIGVLDIDAPITDRFDDNDKEHLEAIVKIIEKQLA"
     gene            18471..20165
                     /gene="ezrA"
                     /locus_tag="O0293_000258"
     CDS             18471..20165
                     /gene="ezrA"
                     /locus_tag="O0293_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HCY0279171.1"
                     /translation="MVLYIILAIIVIILIAVGVLFYLRSNKRQIIEKAIERKNEIETL
                     PFDQNLAQLSKLNLKGETKTKYDAMKKDNVESTNKYLAPVEEKIHNAEALLDKFSFNA
                     SQSEIDDANELMDSYEQSYQQQLEDVNEIIALYKDNDELYDKCKVDYREMKRDVLANR
                     HQFGEAASLLETEIEKFEPRLEQYEVLKADGNYVQAHNHIAALNEQMKQLRSYMEEIP
                     ELIRETQKELPGQFQDLKYGCRDLKVEGYDLDHVKVDSTLQSLKTELSFVEPLISRLE
                     LEEANDKLANINDKLDDMYDLIEHEVKAKNDVEETKDIITDNLFKAKDMNYTLQTEIE
                     YVRENYYINESDAQSVRQFENEIQSLISVYDDILKEMSKSAVRYSEVQDNLQYLEDHV
                     TVINDKQEKLQNHLIQLREDEAEAEDNLLRVQSKKEEVYRRLLASNLTSVPERFIIMK
                     NEIDHEVRDVNEQFSERPIHVKQLKDKVSKIVIQMNTFEDEANDVLVNAVYAEKLIQY
                     GNRYRKDYSNVDKSLNEAERLFKNNRYKRAIEIAEQALESVEPGVTKHIEEEVIKQ"
     gene            complement(20239..20379)
                     /locus_tag="O0293_000259"
     CDS             complement(20239..20379)
                     /locus_tag="O0293_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279172.1"
                     /translation="MIMLFSLLILIKTTHLITFIFKQLLFIFIVPFIYPYKKEARQRI
                     VG"
     gene            20528..21676
                     /locus_tag="O0293_000260"
     CDS             20528..21676
                     /locus_tag="O0293_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCY0279173.1"
                     /translation="MFILIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQA
                     NQLLQQAKTQINAMINSKTNYDVVFTSGATESNNLALKGIAYRKFDTAKEIITSVLEH
                     PSVLEVVRYLEAHEGFKVKYVDVKKDGSINLEHFKELMSDKVGLVTCMYVNNVTGQIQ
                     PIPQMAKVIKNYPKAHFHVDAVQAFGKISMDLNNIDSISLSGHKFNGLKGQGVLLVNH
                     IQNVEPTVHGGGQEYGVRSGTVNLPNDIAMVKAMKIANENFEALNAFVTELNNDVRQF
                     LNKYHGVYINSSTSGSPFVLNISFPGVKGEVLVNAFSKYDIMISTTSACSSKRNKLNE
                     VLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK"
     gene            21676..22899
                     /gene="thiI"
                     /locus_tag="O0293_000261"
     CDS             21676..22899
                     /gene="thiI"
                     /locus_tag="O0293_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCY0279174.1"
                     /translation="MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVK
                     GKRDRMYIELEDHADINEITYRLSKIFGIKSISPVLKVEKTIEAISAAAIKFAQQFEE
                     NSTFKIDVKRADKNFPMDTYELQRELGGAVLKHFDNISVNVKRPDHEIRVEVRLDAIY
                     MYEEVVPGSGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQ
                     AKEKVIELTRILAERVGPIKLHIVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLV
                     HQIGALAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYDKEEIIIKSKEIGTF
                     ETSIQPFEDCCTIFTPKNPVTEPNFDKVVQYESVFDFEEMINRAVENIETLEITSDYK
                     TIKEQQTNQLINDFL"
     gene            22943..23713
                     /locus_tag="O0293_000262"
     CDS             22943..23713
                     /locus_tag="O0293_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HCY0279175.1"
                     /translation="MDLNLTMIIIIILFGFIAAFIDSVVGGGGLISTPALLAIGLPPS
                     VALGTNKLASSFGSLTSTIKFIRSGKVDLYVVAKLFGFVFLASACGAYIATMVPSQIL
                     KPLIIIALSSVFIFTLLKKDWGNTRTFTQFTFKKAIIFAALFILIGFYDGFVGGGTGS
                     FMLFVLLVFGFDFLSAAGNAKVLNFASNIGALVLFMVLGQVDYVIGLIMAISMIAGSY
                     AGAHFAIKQGVGYVKVLFIIITAILILKNAFDYIQQFV"
     gene            23811..24305
                     /gene="tpx"
                     /locus_tag="O0293_000263"
     CDS             23811..24305
                     /gene="tpx"
                     /locus_tag="O0293_000263"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCY0279176.1"
                     /translation="MTEITFKGGPIHLKGQQINEGDFAPDFTVLDNDLNQVTLADYAG
                     KKKLISVVPSIDTGVCDQQTRKFNSDASKEEGIVLTISADLPFAQKRWCASAGLDNVI
                     TLSDHRDLSFGENYGVVMEELRLLARAVFVLDADNKVVYKEIVSEGTDFPDFDAALAA
                     YKNI"
     gene            24428..25375
                     /locus_tag="O0293_000264"
     CDS             24428..25375
                     /locus_tag="O0293_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0279177.1"
                     /translation="MAEQQTIMERLFHTLDEKAKTLNNENGQSFIENLGLAMEQVYTN
                     ERGLLEQSTLQDRRKAFQFAYLSLMQEEKIQANHQITPDSIGLILGFLVERFMNNQEE
                     LHIVDIASGAGHLSATVKEVLPEIAVMHHLIEVDPVLSRVSVHLANFLEIPFDVYPQD
                     AIMPLPLEEADIVIGDFPVGYYPIDERSKEFKLGFEEGHSYSHYLLIEQAINALKDAG
                     YAFLVVPSNIFTGEHVKQLEKYIATETEMQAFLNLPPTLFKNEKARKSILILQKKKSG
                     ETKPVEVLLANIPDFKIPSQFQGFMTELNQWMDTNRPKK"
     gene            25463..26665
                     /locus_tag="O0293_000265"
     CDS             25463..26665
                     /locus_tag="O0293_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCY0279178.1"
                     /translation="MSKLILAINAGSSSLKFQLIRMPEEELVTKGLVERIGLKDSIFT
                     IEVNGEKVKTVQDIKDHVEAVDIMLDAFKAHNIINDINDIDGTGHRVVHGGEKFPESV
                     AITDEVEKEIEELSELAPLHNPANLMGIRAFRKLLPNIPHVAIFDTAFHQTMPEKAYL
                     YSLPYHYYKDYGIRKYGFHGTSHKFVSQRAAEMLDKPIEDLRIISCHIGNGASIAAID
                     GGKSIDTSMGFTPLAGVTMGTRSGNIDPALIPFIMEKTGKTAEQVLEILNKESGLLGL
                     SGTSSDLRDLSEEAESGKARSQMALDVFASKIHKYIGSYAARMHGVDVIVFTAGIGEN
                     SVEIRAKVLEGLEFMGVYWDPKKNENLLRGKEGFINYPHSPVKVVVIPTDEESMIARD
                     VMTFGGLK"
     gene            complement(26911..27411)
                     /locus_tag="O0293_000266"
     CDS             complement(26911..27411)
                     /locus_tag="O0293_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCY0279179.1"
                     /translation="MITYKNILIAVDGSHEAEWAFNRAVGVAKRNDAKLTIVNVIDSR
                     TYSSYEVYDAQFTEKSKHFAEELLNGYKEVATNAGVKDVETRLEFGSPKSIIPKKLAH
                     EINADLIMSGTSGLNAVERFIVGSVSESIVRHAPCDVLVVRTEELPADFQPQVATTQL
                     REKYQN"
     gene            27552..28670
                     /gene="ald"
                     /locus_tag="O0293_000267"
     CDS             27552..28670
                     /gene="ald"
                     /locus_tag="O0293_000267"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HCY0279180.1"
                     /translation="MKIGIPREIKNNENRVGLSPSGVHALVESGHTVLVETNAGSGSF
                     FEDVDYKEAGAEIVAEQAKVWDVDMVIKVKEPLESEYPYFKEGLVLFTYLHLANEEKL
                     TQALIDRKVISIAYETVQLPDRSLPLLSPMSEVAGRMSAQVGAEFLQKLNGGMGILLG
                     GVPGVPKGKVTIIGGGQAGTNAAKIALGLGADVTILDVNPKRLQQLDDLFGGRVHTIM
                     SNPLNIELYVKQSDLVIGAVLIPGAKAPRLVTEDMIKQMKNGSVIIDIAIDQGGIFET
                     TDKITTHDDPTYIKHGVVHYAVANMPGAVPRTSTLALNNATLPYALMLANKGYREAFK
                     SNQPLSLGLNTYKGHVTNKGVAEAFEMEYKSVEEALQL"
     gene            28794..28892
                     /locus_tag="O0293_000268"
     CDS             28794..28892
                     /locus_tag="O0293_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279181.1"
                     /translation="MNDIDLIHYMLLMNIPAYVFLACLLHIAFRLG"
     gene            complement(28986..30041)
                     /locus_tag="O0293_000269"
     CDS             complement(28986..30041)
                     /locus_tag="O0293_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCY0279182.1"
                     /translation="MTKISKIIDELNNQQADAAWITTPLNVYYFTGYRSEPHERLFAL
                     LIKKDGKQVLFCPKMEVEEVKASPFTGEIVGYLDTENPFSLYPQTINKLLIESEHLTV
                     ARQKQLISGFNVNSFGDVDLTIKQLRNIKSEDEISKIRKAAELADKCIEIGVSYLKEG
                     VTECEVVNHIEQTIKQYGVNEMSFDTMVLFGDHAASPHGTPGDRRLKSNEYVLFDLGV
                     IYEHYCSDMTRTIKFGEPSKEAQEIYNIVLEAETSAIQAIKPGIPLKDIDHIARNIIS
                     EKGYGEYFPHRLGHGLGLQEHEYQDVSSTNSNLLEAGMVITIEPGIYVPGVAGVRIED
                     DILVTNEGYEVLTHYEK"
     gene            30216..30905
                     /locus_tag="O0293_000270"
     CDS             30216..30905
                     /locus_tag="O0293_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCY0279183.1"
                     /translation="MKLSFHGQSTIYLEGNNKKVIVDPFISNNPKCDLNIETVQVDYI
                     VLTHGHFDHFGDVVELAKKTGATVIGSAEMADYLSSYHGVENVHGMNIGGKANFDFGS
                     VKFVQAFHSSSFTHENGIPVYLGMPMGIVFEVEGKTIYHTGDTGLFSDMSLIAKRHPV
                     DVCFVPIGDNFTMGIDDASYAINEFIKPKISVPIHYDTFPLIEQDPQQFKDAVNVGDV
                     QILKPGESVQF"
     gene            complement(31210..31623)
                     /locus_tag="O0293_000271"
     CDS             complement(31210..31623)
                     /locus_tag="O0293_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCY0279184.1"
                     /translation="MYKNILLGVDTQLKNEKALKEVSKLAGEGTVVTVLNAISEQDAQ
                     ASIKAGVHLNKLTEERSKRLEKTRKALEDYGIDYDQIIVRGNAKEELLKHANSGKYEI
                     VVLSNRKAEDKKKFVLGSVSHKVAKRATIPVLIVK"
     gene            31828..33126
                     /locus_tag="O0293_000272"
     CDS             31828..33126
                     /locus_tag="O0293_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCY0279185.1"
                     /translation="MTKHEQILDYIESLSIGSKISVRKIAKFLNVSEGTAYRAIKDAD
                     KMGMVATIDRVGTVRIEKRNRNEIEHLTFNEIVNIIDGQVLGGNKGITKMVSKFAIGA
                     MELKDILKYIGPKTLLIVGNREDVQIEALKRGTAILITGGFKPSNKVINFANEHDLPV
                     LSSSYDTFLVANIINKALFNQKIRKDILIVQDIMTPLDDLSVLFDTMKIADYKRMANR
                     TGHTRFPVVNESYKLVGIVTSREMINTKDDDEIDKVMTRNPIYVNAMSTVASCAHMMI
                     WEGIELIPVVSSNKKTVGVINRQDVLKSMQLLGRQPQMGETINDQIAKYITMNQDGIT
                     VEVSPLLINHYGTVSKAAFVSIIEETIQYEMRKFKKGNVMIENLNIVYIKTVPIESHI
                     TVRFGILDVGRNFAKIEVNMHSQNDKVASALVICQMFDEV"
     gene            33387..34328
                     /gene="pde2"
                     /locus_tag="O0293_000273"
     CDS             33387..34328
                     /gene="pde2"
                     /locus_tag="O0293_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pApA hydrolase Pde2"
                     /protein_id="HCY0279186.1"
                     /translation="MISTLNEIMKCIEDNDTIIIHRHVRPDPDAYGSQLGLKYYIQQK
                     FPQKQVFAVGEAESSLSFIGELDNIDDKTYQDALVIVCDTANAPRIDDERYSTGRKLI
                     KIDHHPAVDQYGDINLVNTNASSTSEIIYDLISHFNDEAIVNKDIASVLYLGIVGDTG
                     RFLFNNTSEHTMEIAGKLIGHDIDHNALLNKMMEKDPKMLPFQGYVLQHFELMDDGFC
                     QVKITEDVLEQFGIQPNEASQFVNTIADIKGLKIWVFAVDEGNEIRCRLRSKGQLIIN
                     DIAQDFGGGGHPNASGVSVDSWDEFEQLATALRTKLN"
     gene            34349..37546
                     /locus_tag="O0293_000274"
     CDS             34349..37546
                     /locus_tag="O0293_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCY0279187.1"
                     /translation="MVAYLNIHTAYDLLNSSLKIEDAVRLAVSENVDALAITDTNVLY
                     GFPKFYDACIANNIKPIFGMTIYVTNGLNTVETVVLAKNNDGLKDLYQLSSEIKMNAL
                     EHVSFELLKRFSNNMIIIFKKVGDQHRDIVQVFETHNDTYMDHLSISIQGRKHVWIQN
                     VCYQTRQDADTISALAAIRDNTKLDLIHDQEDFGAHFLTEKEINQLDINQEYLTQVDV
                     IAQKCDAELKYHQSLLPQYETPNDESAKKYLWRVLVTQLKKLELNYDVYLERLKYEYK
                     VITNMGFEDYFLIVSDLIHYAKTNDVMVGPGRGSSAGSLVSYLLGITTIDPIKFNLLF
                     ERFLNPERVTMPDIDIDFEDTRRERVIQYVQEKYGELHVSGIVTFGHLLARAVARDVG
                     RIMGFDEVTLNEISSLIPHKLGITLDEAYQIDDFKKFVHRNHRHERWFSICKKLEGLP
                     RHTSTHAAGIIINDHPLYEYAPLTKGDTGLLTQWTMTEAERIGLLKIDFLGLRNLSII
                     HQILTQVKKDLGINIDIEKIPFDDQKVFELLSQGDTTGIFQLESDGVRSVLKKLKPEH
                     FEDIVAVTSLYRPGPMEEIPTYITRRHDPSKVQYLHPHLEPILKNTYGVIIYQEQIMQ
                     IASTFANFSYGEADILRRAMSKKNRAVLESERQHFIEGAKQNGYHEDISKQIFDLILK
                     FADYGFPRAHAVSYSKIAYIMSFLKVHYPNYFYANILSNVIGSEKKTAQMIEEAKKQG
                     ITILPPNINESHWFYKPSQEGIYLSIGTIKGVGYQSVKVIVDERYQNGKFKDFFDFAR
                     RIPKRVKTRKLLEALILVGAFDAFGKTRSTLLQAIDQVLDGDLNIEQDGFLFDILTPK
                     QMYEDKEELPDALISQYEKEYLGFYVSQHPVDKKFVAKQYLTIFKLSNAQNYKPILVQ
                     FDKVKQIRTKNGQNMAFVTLNDGIETLDGVIFPNQFKKYEELLSHNDLFIVSGKFDHR
                     KQQRQLIINEIQTLATFEEQKLAFAKQIIIRNKSQIDMFEEMIKATKENANDVVLSFY
                     DETIKQMTTLGYINQKDSMFNNFIQSFNPSDIRLI"
BASE COUNT        13016 a         5464 c         6844 g        12225 t
ORIGIN      
        1 aaaatgaaag cgctaacccg atttagtcga caagttttta acagttcgtt attatatgaa
       61 tgtaagtaaa aatttcttag ctacaactta catattataa atgcataaat taaacaaaaa
      121 ggggcgaaaa aagttgactc atttatcaga tttagatatt gcgaatcaat caacactaca
      181 accaattaag gatattgctg catcagtagg tatttcagag gatgcattag aaccttatgg
      241 tcattacaaa gctaaaatcg acattaataa aattacgcca agagaaaaca aagggaaagt
      301 tgttttagta actgcgatga gcccaacacc agctggtgaa ggtaaatcaa cggttacagt
      361 tggtttagct gatgcattcc atgagttaaa taaaaacgtt atggttgcat taagagagcc
      421 tgctttagga ccaacatttg gtatcaaagg tggtgcgact ggtggtggtt atgcgcaagt
      481 cttacctatg gaagatatca acttacattt caacggagat ttccatgcga ttacaactgc
      541 aaataatgca ttgtctgcgt ttatcgataa tcatattcac caaggtaacg aattaggaat
      601 cgatcaaaga cgtattgagt ggaaacgtgt attagatatg aatgatcgtg cacttagaca
      661 tgtaaacgtt gggttaggtg gacctacaaa tggtgtacca cgtgaagatg gctttaatat
      721 tacagtagcg tctgaaatta tggcgatttt atgtttaagt agaagtatta aagacttaaa
      781 agataaaatt agtcgtatta ctattggtta cactagagat cgcaagccag ttacagttgc
      841 agatttaaaa gtggaaggtg cacttgcaat gattttaaaa gatgcaataa aaccaaactt
      901 agtacaatca attgaaggga cacctgcatt agttcatggt ggaccatttg cgaatatcgc
      961 acacggttgt aactcaattt tagcaactga aacagcacgt gatttagctg atatcgttgt
     1021 aacggaagct ggatttggtt cagacttagg cgctgaaaaa ttcatggaca ttaaagtgcg
     1081 tgaagcagga tttgatccgg cagctgtcgt tgttgttgcg acaattcgtg cgttaaaaat
     1141 gcatggtggt gtagcgaaag ataatttaaa agaagaaaat gtagaagcag taaaagcagg
     1201 aattgttaat ttagagcgtc atgttaataa tattaaaaaa ttcggtgtag aaccggttgt
     1261 tgcaattaat gcatttatac atgataccga tgcagaagta gaatatgtaa aatcttgggc
     1321 taaagaaaat aacgtacgaa ttgccttaac tgaagtttgg aaaaaaggtg gtaaaggtgg
     1381 cgttgactta gcaaatgaag tattagaagt cattgatcaa cctaattcat ttaaaccttt
     1441 atatgaatta gaattaccat tagagcaaaa gattgaaaag attgtgactg aaatctatgg
     1501 cggttcaaaa gtaacgttta gcagtaaagc gcaaaaacaa ttaaaacaat ttaaagaaaa
     1561 tggttgggat aattacccag tatgtatggc gaaaacacaa tattcattct cagatgatca
     1621 aacgttgtta ggtgcaccat caggatttga aattacaatt cgtgaattag aagcgaaaac
     1681 aggtgcagga tttatcgtag cgttgacagg tgcaatcatg actatgcctg gtttacctaa
     1741 aaaaccagca gcattaaaca tggatgttac tgatgatggt catgcaattg ggttattcta
     1801 ataaatcatg tcaattgttt aataaagata agtaaatagt ttaatagacc ggactgttgg
     1861 agatgcatta tttcagcagt tcggtttttt gctgtgctaa aaatagattc aatttggcga
     1921 atctaacgac aatgtttgaa ggtggttaat taatgtatat gaagataaaa agtgggcttg
     1981 aagaatagga aagcgatgca atgaatattc catattaaaa aaaaataata aaataggttg
     2041 caatatttaa ttgggatgcg ctacaattaa cactaataat aggagttgtt aacaacatga
     2101 acaaacatca cccaaaatta aggtctttct attctattag aaaatcaact ctaggcgttg
     2161 catcggtcat tgtcagtaca ctatttttaa ttacttctca acatcaagca caagcagcag
     2221 aaaatacaaa tacttcagat aaaatctcgg aaaatcaaaa taataatgca actacaactc
     2281 agccacctaa ggatacaaat caaacacaac ctgctacgca accagcaaac actgcgaaaa
     2341 actatcctgc agcggatgaa tcacttaaag atgcaattaa agatcctgca ttagaaaata
     2401 aagaacatga tataggtcca agagaacaag tcaatttcca gttattagat aaaaacaatg
     2461 aaacgcagta ctatcacttt ttcagcatca aagatccagc agatgtgtat tacactaaaa
     2521 agaaagcaga agttgaatta gacatcaata ctgcttcaac atggaagaag tttgaagtct
     2581 atgaaaacaa tcaaaaattg ccagtgagac ttgtatcata tagtcctgta ccagaagacc
     2641 atgcctatat tcgtttccca gtttcagatg gcacacaaga attgaaaatt gtttcttcga
     2701 ctcaaattga tgatggagaa gaaacaaatt atgattatac taaattagta tttgctaaac
     2761 ctatttataa cgatccttca cttgtaaaat cagatacaaa tgatgcagta gtaacgaatg
     2821 atcaatcaag ttcagtcgca agtaatcaaa caaacacgaa tacatctaat caaaatacat
     2881 caacgatcaa caatgctaat aatcaaccgc aggcaacgac caatatgagt caacctgcac
     2941 aaccaaaatc gtcaacgaat gcagatcaag cgtcaagcca accagctcat gaaacaaatt
     3001 ctaatggtaa tactaacgat aaaacgaatg agtcaagtaa tcagtcggat gttaatcaac
     3061 agtatccacc agcagatgaa tcactacaag atgcaattaa aaacccggct atcatcgata
     3121 aagaacatac agctgataat tggcgaccaa ttgattttca aatgaaaaat gataaaggtg
     3181 aaagacagtt ctatcattat gctagtactg ttgaaccagc aactgtcatt tttacaaaaa
     3241 caggaccaat aattgaatta ggtttaaaga cagcttcaac atggaagaaa tttgaagttt
     3301 atgaaggtga caaaaagtta ccagtcgaat tagtatcata tgattctgat aaagattatg
     3361 cctatattcg tttcccagta tctaatggta cgagagaagt taaaattgtg tcatctattg
     3421 aatatggtga gaacatccat gaagactatg attatacgct aatggtcttt gcacagccta
     3481 ttactaataa cccagacgac tatgtggatg aagaaacata caatttacaa aaattattag
     3541 ctccgtatca caaagctaaa acgttagaaa gacaagttta tgaattagaa aaattacaag
     3601 agaaattgcc agaaaaatat aaggcggaat ataaaaagaa attagatcaa actagagtag
     3661 agttagctga tcaagttaaa tcagcagtga cggaatttga aaatgttaca cctacaaatg
     3721 atcaattaac agatttacaa gaagcgcatt ttgttgtttt tgaaagtgaa gaaaatagtg
     3781 agtcagttat ggacggcttt gttgaacatc cattctatac agcaacttta aatggtcaaa
     3841 aatatgtagt gatgaaaaca aaggatgaca gttactggaa agatttaatt gtagaaggta
     3901 aacgtgtcac tactgtttct aaagatccta aaaataattc tagaacgctg attttcccat
     3961 atatacctga caaagcagtt tacaatgcga ttgttaaagt cgttgtggca aacattggtt
     4021 atgaaggtca atatcatgtc agaattataa atcaggatat caatacaaaa gatgatgata
     4081 catcacaaaa taacacgagt gaaccgctaa atgtacaaac aggacaagaa ggtaaggttg
     4141 ctgatacaga tgtagctgaa aatagcagca ctgcaacaaa tcctaaagat gcgtctgata
     4201 aagcagatgt gatagaacca gagtctgacg tggttaaaga tgctgataat aatattgata
     4261 aagatgtgca acatgatgtt gatcatttat ccgatatgtc ggataataat cacttcgata
     4321 aatatgattt aaaagaaatg gatactcaaa ttgccaaaga tactgataga aatgtggata
     4381 aagatgccga taatagcgtt ggtatgtcat ctaatgtcga tactgataaa gactctaata
     4441 aaaataaaga caaagtcata cagctgaatc atattgccga taaaaataat catactggaa
     4501 aagcagcaaa gcttgacgta gtgaaacaaa attataataa tacagacaaa gttactgaca
     4561 aaaaaacaac tgaacatctg ccgagtgata ttcataaaac tgtagataaa acagtgaaaa
     4621 caaaagaaaa agccggcaca ccatcgaaag aaaacaaact tagtcaatct aaaatgctac
     4681 caaaaactgg agaaacaact tcaagccaat catggtgggg cttatatgcg ttattaggta
     4741 tgttagcttt attcattcct aaattcagaa aagaatctaa ataattaact aaatatagca
     4801 tatgtatgat taactttgta gacaatgtga aagcaattaa tttataaact attgattggt
     4861 ttaatggctt tcctttagag taaataaaaa gaacagcagt gagaaatttt ctaattgaaa
     4921 ataatcttac tgctgttttt aatatttgga ttcattgttg tggttacttt aaaaagtgag
     4981 catcaattaa cgcttttttc gatttaacaa atgtgattta atatcatatt ttaatgcgtc
     5041 gttgtattct ttttcagtga tttgatcttc gattaacata cgctttaata cataatgttg
     5101 tctttgaata ctatatttca aatctttatc cgattttaac gttccatctt tttcgtaggg
     5161 tgtatagcca tatgggcttt gcaacaaacc gataaggtat gcagattgtg caattgataa
     5221 atcttttggt ggaataccaa atagactata tgaagcggat gcaattccgg aaatattagc
     5281 gccattataa tctctaccga agggaactat atttaaatat gtatatataa tttcatcttt
     5341 tgagagtagg tgttctaatc taattgctag gcgaagttca tttgcttttc tactatatgt
     5401 tttttcgttg gtaagaactt gatttttaac aagttgttgt gtaattgtgc taccacctga
     5461 actttgatca gtattaaaaa tatcttgtat cattgctctt aaaatcgcct ttggtaagat
     5521 gccatcatgt ttataaaata aagtgtcttc agatgacgtt aatgctttaa tgacatttgg
     5581 acttgatgtt ttagggccta taatgagtga gttttgagaa tggttatact catataataa
     5641 atttttgtta ttatgatcta ataattcatc gccaggtatt tgtcgaactt tttttattaa
     5701 agcatcatct gataatgagt cggacgtttt agttaaatga tgaaaatata aagacatcgc
     5761 aatcacagcg atagcaacga tggcaatgaa aatcataaat ataaaaacaa tgacatgttt
     5821 gatagcttta tatatctttt caaaatgata tatacgatga ttcaattgat gattgttgtc
     5881 ttgattcgtc atctttaacc tcctaaatta cgtatagtat accatattta agcaatttga
     5941 cttttatttt aacgttaatt ataattaggg aaaatgcata aagaatagga taaaaggaca
     6001 agtaatatta tttgaaatca tcagagagct agtggtcgct gtgaactagt gtttttaata
     6061 atatgaatac acctttataa ctaacatgtc ctacgtttat taatgaacgt atcattaata
     6121 gagtgaacat tatcttatga ttatgtttaa gtttggtggt aacgtgcaat cgcgccctta
     6181 catatttgtt gtaagggttt tttttacgtt caaaaaactt taaagtattt taacaagtat
     6241 tcaataaata attataaata ggaggaaata ataatgacga atgtattaat tgaagattta
     6301 aaatggagag gtcttattta tcaacaaact gatgaacaag gtattgaaga tttattaaat
     6361 aaagaacaag tgacgttata ctgcggtgcc gatccaacgg cagatagttt acatattggt
     6421 cacttactac cattcttaac attaagacgt tttcaagaac atggacatcg tcctatcgtt
     6481 ttaattggcg gtggtacagg tatgattggt gatccatcag gtaaatcaga agaacgtgtg
     6541 ctacaaacag aagaacaagt agataaaaat atcgaaggta ttagtaagca aatgcacaat
     6601 atttttgaat ttggaacaga ccatggtgca gtgcttgtta ataatagaga ctggttagga
     6661 caaatctcat taattagttt tttacgtgac tatggtaaac acgtcggcgt taattacatg
     6721 ttaggtaaag attcaatcca aagtcgttta gaacatggta tttcatatac agaattcaca
     6781 tacacgattt tacaagctat tgatttcggt catttgaata gagaattgaa ttgtaagatt
     6841 caagtaggtg gatcagatca atggggtaat atcacaagtg gtattgaatt aatgcgtcgt
     6901 atgtatggtc aaacagacgc atacggttta actattccgc ttgtaactaa atcagatggt
     6961 aagaaatttg gtaagtctga gtcaggtgct gtttggttag atgctgaaaa aacaagtcct
     7021 tatgaatttt atcaattctg gattaatcaa tcagacgaag atgtaattaa attcttaaaa
     7081 tactttactt tcttaggaaa agaagaaatt gatcgcttag aacaatctaa aaatgaagca
     7141 ccgcatttac gtgaagctca aaaaacatta gctgaagaag taactaaatt tattcatggt
     7201 gaagatgcat taaatgatgc aatccgtatt tcacaagcat tatttagtgg tgatttaaaa
     7261 tcattatcag cgaaagaatt aaaagatgga tttaaagatg tgcctcaagt gacattatca
     7321 aatgacacaa caaatatcgt tgaagtcctt attgaaacag gcatttctcc ttctaaacga
     7381 caagcacgtg aagatgttaa caatggtgcg atttatatta atggtgagag acaacaagat
     7441 gttaattatg ctttagcacc agaagataaa attgatggcg aatttacgat tattcgtcgc
     7501 ggtaagaaaa aatacttcat ggttaactat caataaatat aattgcatag ctaaataaat
     7561 tagagcctac tcatattcat tcctaagaat gtaatgagta ggctcttaat gtacttttct
     7621 gtctgtaaat tatctaaaga aatctctatc gccttggcca ggtgattgac gttctgatcg
     7681 gctttgacgt tttggttgtt ctttttgttg ttttaatttc actttaactt cttttgtttt
     7741 accatcacgg ataacggtaa cagtgactga ttcaccaggt tttttatttt catataaata
     7801 gcttcttaaa tcaacatcat ctttaatttt cttgccatca atttctgtaa taatatcacc
     7861 ttttttaaga tcaatatcac tatcagcttt ggcgacataa ataccgtctt ctctatcagt
     7921 atgaagttgc tcgcgctctt cttcaggaat atctttcaaa ttaattaaac caataccaat
     7981 cgaagggcgg tcaattttac catgttttac aagttgttca attgttactt taacttcatt
     8041 acttggaata gcaaacccga taccttcaac ttgtgtcgca gcaattttca ttgagttaat
     8101 accaactaaa ttaccattaa tatctactaa tgcgccacct gagttacctg ggttaatagc
     8161 agcatctgtt tgaagaacgc taacttttgt attgccacca gttgtctcag cgtcaatcgt
     8221 acgttcgctt gctgaaatga taccagatgt tacagagtta gcaaattgta atcctaatgg
     8281 gttacccatt gcgaatacgc tatcgccagt ttgtactttt gaagagttgg caaattgaat
     8341 cgctttaata ccttttgtat tttcaatttt aagtacagca atatcagtta ctgcatcttt
     8401 accaactaat ttcgctttaa cttgtttttt attatgtaat tggactctaa tttcatttgc
     8461 gccatcaata acatgattgt ttgtaacgat ataagctgaa ttgttgttta tttgatagat
     8521 aacacctgaa cctactccag cttcagatgg tttagatgat ttgcctttta ataagtcgtc
     8581 tacacttgat gctttttgca tgttaataac tccaacaatt gtaggggaga cagattttat
     8641 catttcatga acggtaccga atttcttgct ttgaccgtct aattgattgc cacctttatt
     8701 atttgttgtc tgaacagttg aaccatcttt atttaaaatt gtactattta atactttgcc
     8761 tataccaagt actagaagtg caccaataat tccagcaatc aatgcaacga tgactgtttt
     8821 aaaccatgga aatttaggtc ttctgtatct aggtgtttgg ctatggtttg ttgtagaatg
     8881 atctgtatga ttaaaatctg acatacttaa cctccattat atgatttata tatgctttaa
     8941 ttatgtcttt tttttataat tattgctagt aatacactat acaactttag atgtattttc
     9001 agactatctt gttctagtaa aaatatagtg ataaaatcta gttgttataa acaaataagt
     9061 taagtaggtg gcacaatgta ttcagtgatt agtaagattt tgaatttcat tttagttaaa
     9121 atgtcaaaat cattatacgt aatcggtaaa gataatattc ctaaggatag taaatatgtc
     9181 gtaacttgta cgcatgaaag ttataacgaa gtcattatgc taggtatggc attatatcct
     9241 aatcaaattc attatatggc taaaaaagaa ttattcaaaa ataaatggat tggtaaattt
     9301 ttaacgtctt taaatgcatt tcctgtagat cgtgaaaatc caggacctag tactttaaaa
     9361 cgcccaatca atttgttgaa agataataag acagttggta tcttcccaac aggttctcgc
     9421 acatcgcaag aaggtgcacc tttgaaacgt ggtgcttcaa caattgcaat gttaagtaag
     9481 tcaccaatat tacctgttgc ttatgttggt cctactaaga ttcacggtct tttaacagga
     9541 caagcataca taaatattgg caaaccaatc gatattaacg atttacctaa agatttaaaa
     9601 cgaaacgagc gtatcgatta tattacaaaa gagattgaaa cacgaacagc aaagttacaa
     9661 caagaattgc atgaaattgt aaaaagttta taaaataact tgaggtagaa atatagctgg
     9721 ttagtaagcc taggtgacta taacggagta tcgaagtagt tattctagtt acattagtaa
     9781 tctttatatt tctacattat tttaagaagg atgacattca atgtataaat ttttccaaaa
     9841 tttagggcgt tcgttaatgt taccagttgc cattttaccg gctgcagcaa ttattgccgg
     9901 aattggtaat acgttaaatg cacttcatgc gacgccgaaa atcgcaatgt tctttactac
     9961 ggtaggtact acgattttag agcaattagg tattttattt gcaattggtg tagctatagg
    10021 aatggcaaag aaaaatgatg gtgtagtcgc gcttgcagct acgttaggat attttttagt
    10081 tacagttgta ttatcaccaa tgaaattagc gcctttatta ggaatgaaag cttcggaaat
    10141 aaattcagct tttgaaaaga tgaataatgg caatgtcttt gttggtattg taataggttt
    10201 aatagcagct tatgcataca ataagtttag tgaaacagaa ttaccattag cattatcatt
    10261 ttttagtggt aaacgtctag ttccaattat gactgcattt tactgtacat ttttagttgt
    10321 catattgtta ttcttatggc cactacttta ttcatggatt gtaaaatttg gtgaatctat
    10381 agtcggttta ggatcgtttg gagctttcat atatggtgtt gccaatagat tattaattcc
    10441 tacaggatta catcatgcac tgaatagtgt gttctggttt gatacgattg gtattaatga
    10501 tattggtaaa ttccaaagtg gcaaagatgc tataaaaggt attacaggac gctatcaagc
    10561 gggattcttc ccaatcatga tgttcggtat tccagcggct gcgttagcaa tgtatcatac
    10621 agctaaaact actcagaaaa aacaagttta cggttggttc ctagcgagtt cagttgcagc
    10681 atttttcgtt ggtgttacag agccgattga gtttgcattt atgtttgttg cgccaatctt
    10741 atatgttgtt catgcgttat taacaggatt atctttattt atcgcagcta cattccattg
    10801 gacagctgga ttttcattta gtgcaggttt gatagattat gtgttatcac taattaatcc
    10861 agtatcaaat catccattga tgttgcttgt tcaaggtgtt gtatttttca tcttatacta
    10921 tgtgattttc cgtgtagtaa tccaagtatt taacttgaat acgattggta gaggtgaaaa
    10981 tgaattagtt gacccaacag ttgtaaaaga taatattgct cctggtgaaa atgatattaa
    11041 acaaagtaaa tatcatcaac atgctataca aatattagaa ggtttaggcg gtcaagagaa
    11101 tattgttaat ttaaccaatt gtgcaacaag gttgcgtcta gagttaaaag acacatctat
    11161 tattgataaa cagaagatta aaaacgccgg tgcagttggt gttactgtta atggcaaaca
    11221 ttcaactcaa gttattgtcg gtacacacgt acagcaggtt gctgatgaaa ttgaaaaaca
    11281 tttataaaat ttaaacagct aaagcgcaaa tctaacttca atagggtttg cgcttttttt
    11341 agtcaaatag tagaatgatt tatatctgaa ttttcagaaa tggagtgggg attgtgaaga
    11401 aaattttatt tgatgttgat ggcgtttttt taagtgaaga acgttgtttc gatgtctctg
    11461 cattaacggt atatgaatta ttaatggata agtgttattt aggtcttcat tcacatatag
    11521 attgggaaac tttgactgat aacgatatac aagacattag gaatcgaatt tttcaaaaag
    11581 ataagatttt aaacaaatta aaatcgctag ggctgaattc aaattgggat atgttattta
    11641 tcgtttttag tattcattta attgatattt tgaaaaagtt atcacatgat gaaattgagg
    11701 cattcatgta tcaagatgag cctgtcgaat tgaagttgca aaatataagc acaaacttag
    11761 cagactgttt taatttaaat gaacaactac ctttacaatt tttggataat gtaaaagttg
    11821 gtaaaaataa tatttatgct gctttggaag agtttgcaac aacagagtta catgtttcgg
    11881 atgctacttt atttagtttg aagggtgcat tatggacgtt agcgcaagaa gtttatcaag
    11941 aatggtattt aggatcgaag ttgtatgaag atgttgaaaa gaaaatagca cgaactactt
    12001 ttaagacagg ttatatttat caagaaatta ttttgagacc agtagatgaa gttaaggtac
    12061 ttctgaatga tttaaaaggt gctggtttcg aattaggtat tgcaacaggt cgtccttata
    12121 ctgagactgt tgtgccattt gaaaatttag gattgttacc atattttgaa gctgatttta
    12181 ttgcaacagc aagtgatgtt ttagaagcag agaatatgta tccgcaagca cgaccattag
    12241 gaaagccgaa tccttttagt tatatcgcag ctttatatgg taataatcgc gataaatatg
    12301 aatcttatat caataagcaa gataacattg taaataaaga tgacgtattt atagtaggcg
    12361 attcgttagc tgacttatta agtgctcaaa aaataggtgc aacgtttatt ggaacattaa
    12421 caggtttaaa aggtaaggat gctgcaggtg agttagaagc gcatcatgcc gactatgtta
    12481 ttaatcattt aggtgaactt agaggtgtac tagataattt gtaatttgat tgttgtttga
    12541 cagcataact tgtagtgaat gattgaacca aaggtttcat attgagttac aatgaaatta
    12601 ataatgaaaa aatgccaaga agcaatggaa gtaatccaat gtcttcttgg cattttgaat
    12661 ttacataaat tgtttatgac tgtaccgtca attcagttgt gaaaatttga ttgtattcac
    12721 caacttgttt aagttcatca attatattgt ttgaaacagg ttgatcaacg gataaaatca
    12781 ttagcgcatc tccgcccgct tcagttctac ctaaagtcat agatgcaatg ttgatattgt
    12841 atttacctaa caatgcgcca gtttttccta ccatacctgg agtatcatta tgatatgaca
    12901 caatttgata ttgatttggc tttaagtcta cagaaaaatt attaattcta acaattcttg
    12961 gaccgaaacc tgtaaagaca gaagcgccaa ctttaacgga atcgctatcg tttgatagtt
    13021 ctacctctaa gtagttacta aaacctgtct ctgctttatt attttcaata tttaatgtca
    13081 cttgttgttg atttaacaac attagagcat tgataatatt gacttcagga ccaagatcat
    13141 cttttaaaat atgagtaata attgtacgtg ttaataaact actatcaata gaggctaagt
    13201 cgccaccgta cgtaatttta atagagcttg gtgcattgta cattaattga atagctaatt
    13261 caccaactgt ttggcttaaa ttgatgaatg attttacagt atcatctata ttgcttaagt
    13321 ccattttagg tgcattcact gcatgcgtta cagtaccatc aattaaaatt tcgatgattt
    13381 catttgaaac agaaattgcc actttttctt gagcttcgac tgttgaagca cccaaatgag
    13441 gtgtaacaat aattttatca tgtgcaacaa gaggcgagtc agttgcaggt tcatgttcaa
    13501 acacatcgat agctgcccga ctaatttgtc cttcgtctaa tgcttttatt agcgcctttt
    13561 catcaataat accaccacgt gccacattga ttatttgcaa actaggtttt gctttggcaa
    13621 aaaagacagc attaattaag ccttttgttt taggtgttag tggtgtatgt aatgtaacga
    13681 aatcagaatg ttgggcaatc tcatcaactg ttgccttcgt aatgcttaaa gattttgctt
    13741 tttcatccgt taagtaaggg tcaaaagcta gtattttcat tccgaaactt tgcgcacgtt
    13801 tagcaacacc taaaccaatt ctaccagcac caatgacacc taatgtttta tgataaagct
    13861 cagtaccttt aaatgcattt cgattccatt ctttatttgt aagtgattgg tgtgcttgcg
    13921 gaatatttcg tgccattgat aataacattg ccagtgtatg ttcagtagct gaaatcgtat
    13981 taccatctgg ggcattaata actaaaatac cttttaatgt tgcagcatca atattaatat
    14041 tatctacacc aacaccggcg cgtgcgatta cttttaaaga atcagcagca tttatgatat
    14101 tttcagtaac cgtagtttga ctacgaacga ttaaagcatg gtatgaaggt ataattttga
    14161 ttaatgcttc ttcggacaag ccagtttgaa tatctacatt gaattgttcg tgatctaata
    14221 atgctttgat accatctttt gatatgggat ctgcaacgag tacattaaat tgcttcatga
    14281 ataacctcca tatattttga tataccttta ccgatatagt taactttacg gtgttcagtt
    14341 aaaataattt ctaaagcaga tactaccgat aaaatatcga aaggactaat tttccccata
    14401 tgaccaattc ttaaaatttg acctttaaga tggccttgac caccagcaat tgttatttta
    14461 aagcgatttt taagttcatc tttgattatt ttcacttcat catttgtatt aggtttgaat
    14521 gctgtaaccg ttggagatgc atctttatca ttgactaata aagttaaatc taatgctttt
    14581 aaggcgcttc ttaatgcatt tctaattgca tagtgtcgtg ctattacgtg attgaaacct
    14641 tctgctttta cggtttcaac gtatgcattt acacctctaa ataaagacac atttggtgtg
    14701 aacggtgtag aattgtcagc ttgtgacgaa atgtatttat ttaagtctag ataaaatttt
    14761 ggcgtagtta cttctttgaa atgttctttt gcacggtggc tataagctac aaaagctaat
    14821 cctggaggta acataattgc tttttgacta ccagaaacaa gtacatcaat tttatctttg
    14881 ttaatgtcaa catcaacagc accaatgcaa cttacgccgt caactacaaa ataaatatta
    14941 ctattaaatt gattaatggc atttcctaac tcgtgaatag ggtgtagcac tgtcgtagat
    15001 gtttcgcaat attgactaaa tactgcttta acttcaacat ttaaagttga aaggaaattg
    15061 atgaaatctt tgacatctac agcttctccc catgttacgt cataaatatg cacatttttg
    15121 taataagttt gtgcaatttg tttaaatcgg ttaccaaagg cacctgaaac aatgacaacg
    15181 aagtgatctt cagggtttac aatgttcaac atactagcct ccaagacgct tgtaccgcta
    15241 gatgttaaaa taagtacatc attttgactc ccaaatattg gctttagacc ttgaaatgct
    15301 tgttgtgcga tgtcttcaaa atctttagaa cgatgaccaa ccataggtgc ttgaatttct
    15361 ctcataatgg catcaggtac aggggttggg ccaggtgtta ataacaacgg ttgatgataa
    15421 tacatttata tgcctcctat aaataagatt tcactatttt agcaaatttt ctgaaaattt
    15481 aaaagcctaa aaaagtgata acgctatatt gtcgaacaaa cgttatcact taacgatttt
    15541 atttggcctg aatgatggga tagattttta tgtccacatt atttcttaca gcatttgaaa
    15601 tcatgcaatt attatctgca attgttatca attttggtaa tcgcttttct aattgtgcta
    15661 tttgatcact tggaatttga atttgaggat ggtgcacaat ttttgacata ctgaattttc
    15721 cgttatttaa acaagctgtt ccaatcgatt gttgttcaat tgaaatatct gtgaactttg
    15781 cacgttcaag agtagctgct aatgagatga tataacatga tgaagcggct gatactaaca
    15841 tttcatcggg atttgttcct ataccaacac cacctaaaga agcaggtata gaaatattct
    15901 ctgaaagtat gtcgccttga acgtttccga cattgttacg accaccttgc caagaagttt
    15961 ggactttaaa gtcatgttga tgcaattgct ttaacctcca atataattgt gatagtttaa
    16021 ttttagaata gtttatcaaa gtttaacaga aaggtgactt atcaatgact ctgaataaac
    16081 tgaaagatga attacaaatt gtttcgcacc gtggattgcc gagtgatttt cctgaaaata
    16141 caatggtcgg ttatcgagag gtaatggggc tcaatgttgc tatgttagaa atagatgttc
    16201 atttgaccaa agaccaacat tttgttgtga tacatgatga aacaattgat agaacatcgg
    16261 atggtaaggg gcgtattgct gattacacat tatcgcaatt aaaatcattt gattttggta
    16321 gttataaaga tgttgctttt aagggagaac gaatacccac gttagatgaa gtactttcat
    16381 tatgcttaaa gtatgataaa aagctattga ttgaattgaa aagtccgaat ttatatccag
    16441 aaatagaatg taaattgctg gcatttttgg aagaaaagaa agtcgatgcg acacaagtag
    16501 ttatacaatc ttttgatatc gaatgcatcg aaaagttaaa cacattgggc agtatatatg
    16561 aattaggtgt gctttgtagt aaacgtaatt attggtataa aaagccaaac ttttcaagaa
    16621 ttgctcaaat tgctagttat gttaatccta attatgcatt agtgaccaga aaatttgttg
    16681 ataaagcaca tcatcatcaa ttacaggtta tgccatacac agttaataaa ttgaaaactg
    16741 gagaaaaatt aagacaaatg ggtgtagatg gattaattac agataatccg aaattattta
    16801 ttaagtgaga cgatgaatgg taatttgata gagggatgag gattcgtata tcgcaattga
    16861 taattttaaa aactaatgat gaaatactaa agataattaa acaaataaga cttgtaaatt
    16921 ctaagttgat tgtaaaatag ggtagctcca tataattttg aatctatcat ttttaaatta
    16981 ataaaaaaaa cacccacaat tgtgggtgtt tgtattgaac gtattatctt gagtagtact
    17041 caacgattaa ttgttcgtta atttcagcag gtaattcgct acgttctggt aaacgtacga
    17101 aagtaccagt taagctgtca gcatcaaagt ttaagtactc aggtacgaaa ttgttgattt
    17161 caactgattc aacgatgatg tttaattttt gagatttttc acgaactgaa attgtttgac
    17221 caggtttaac agaataagat ggaatatcaa cacgtttacc atctactaag atatgaccgt
    17281 ggttaactaa ttgacgtgct tgacgacgag tacgagctaa acctaatgaa taaacaacag
    17341 cgtctaaacg acttgctaat aagatcatga agttttcacc gtgtacaccg aattttttac
    17401 cagcgatgtc aaatgtgtta cggaattgtc tttcagtcat tccatataag taacgtaatt
    17461 tttgtttttc acgtaattgt aaaccatatt ctgataattt tttacgttgg tttggaccat
    17521 gttgtcctgg tgcgtaagga cgtttttcta attctttacc agtaccgctt aaagagatac
    17581 ctaaacgacg agattttttc cagtttgaac ctctgaatcg agccataata agactcctcc
    17641 tttaattctt ttttgttgtt atgaataaac aaaaaagagt gttgcatgct tactaagata
    17701 tattgtttta tgtgtcttca ccttatagcg acagttacgt gacacgtccg cattgggaac
    17761 aacatagcgc tcaataatat acaactgcta ctttcgttaa ttcacacaaa gattattgta
    17821 acattttcct gctaactgtc aacactttat tgaaaatgct gatgtccttt tatgcgagtt
    17881 gcttttcaat aattttaaca attgcttcaa gatgttcttt gtcattgtca tcaaatcgat
    17941 ccgttatagg ggcatcgata tctaagacgc cgataatttt atcatctttg aaaattggaa
    18001 cgacaatctc agacttacta ttagcatcac aagcgatatg tccttcgaat tgatgaacat
    18061 cagctacaat ttgtgtacga cgttctgaaa cggctgtacc acatacacct tttccaattg
    18121 gaatgtggac acaagcgggg tgtccttgga aaggtccaag tataagttca ttttgttcca
    18181 ataagtaaaa gccaacccaa ttaatttgat ctagattatc atttaataag gcagacatgt
    18241 tacttaaaat agcaatcatg tgatgttcat cttcaataag gcttgctgct tgtttcttta
    18301 ataatgtgta gtttgttggg ttaattgttg tcatatttaa aacctcttat tttcatagat
    18361 tttataatag tatagaattt cgaaaatatt atggcaataa attcatttta taatttatcc
    18421 ttaagcaaaa ttacgttata atagtaatga taaattagga ggagaagcat atggtgttat
    18481 atatcatttt ggcaataatt gtgattatat tgattgctgt aggtgtatta ttctatttac
    18541 gttcaaataa acgacaaatt attgaaaaag caatcgaacg taaaaatgaa attgagacgt
    18601 taccttttga tcaaaacctt gcacaattat ctaagttgaa tttaaaaggt gaaacaaaaa
    18661 cgaaatacga tgcaatgaaa aaggacaacg tagaaagtac aaataagtat ctagctcctg
    18721 tggaagaaaa aatccataat gctgaggctt tattagataa atttagtttc aacgcatctc
    18781 aaagtgaaat tgatgatgca aatgagttga tggatagtta cgaacaaagc tatcagcaac
    18841 aattagaaga tgtaaatgaa attattgcgt tatacaaaga taatgatgaa ttatatgaca
    18901 aatgtaaggt tgattatcgt gaaatgaaac gtgatgtttt agcaaatcgt catcaatttg
    18961 gtgaggcagc aagtctactt gaaactgaaa ttgaaaaatt tgagccaagg ttagagcaat
    19021 atgaagtact aaaagctgat ggtaattatg tacaagcgca caaccatata gctgccttga
    19081 atgaacaaat gaaacagcta agatcttata tggaagaaat accagaatta attagagaaa
    19141 ctcaaaaaga attacctggt caattccaag atttaaaata tggttgccgt gatcttaaag
    19201 ttgaagggta tgatctggat cacgtgaaag tagacagtac attacaaagc ttaaaaacag
    19261 agcttagttt cgttgaacca ttaattagcc gcttagaatt agaagaagct aatgataaac
    19321 tagctaatat caatgataag ttagatgaca tgtatgattt aattgaacat gaagttaaag
    19381 ctaaaaatga tgtcgaagaa acaaaagata tcattacgga taacttattc aaagctaaag
    19441 acatgaatta tacattgcaa acagaaattg aatatgtacg tgaaaactac tatataaatg
    19501 aatctgatgc tcagagtgtt cgtcaatttg aaaatgaaat tcaaagttta atttctgtat
    19561 atgatgatat tttaaaagaa atgtctaaat ctgctgtgcg atatagcgag gttcaggata
    19621 atttacaata tttagaagat catgtcacag ttattaatga caaacaagaa aagctacaaa
    19681 atcatctgat tcaattgcgt gaagatgaag cagaagcaga agacaatctg ctacgagtac
    19741 aatcgaagaa agaagaagtg tatcgtcgat tacttgcttc taacttaaca agcgttcctg
    19801 aaaggtttat catcatgaaa aatgaaattg atcatgaagt tcgtgatgtt aacgaacaat
    19861 ttagtgaacg tccaatacac gttaaacagt taaaagataa agtgtctaaa attgtgattc
    19921 aaatgaatac atttgaagat gaagcaaatg atgttcttgt taatgctgtt tatgcagaga
    19981 aattaattca atatggaaat agatatcgta aggactatag caatgttgat aagagcttaa
    20041 atgaagctga acgattattt aaaaataatc gctataagcg tgcgattgaa attgcagagc
    20101 aagctcttga aagtgttgag ccaggtgtta ctaaacatat tgaagaagaa gttattaagc
    20161 aatagaaact agtatgtagt tatacttaaa taatatgagc actctgtcaa attggactga
    20221 tgagtttaat aattgaagtt agccaacgat acgttgtcta gcttcttttt tatatggata
    20281 aatgaaaggg acaataaata taaatagcaa ttgtttaaag ataaacgtaa tcaaatgtgt
    20341 tgttttaatt aatataagta gtgaaaaaag cataatcaca cagctgttta aatagagtga
    20401 aatagtctaa ttcttattta ataagtagaa ataagattat tttgcgctat aacctttatt
    20461 ttttgtcgga taatagtata atgcttgagt aaaacaattt taacgattga tgattaatga
    20521 aagagaagtg tttattttga tatatctaga taatgcggca acgacgaaag catttgaaga
    20581 agtgttagat acttatttaa aagtaaatca atcaatgtat tataatccga atagtccgca
    20641 taaagctggt ttgcaggcaa atcaattact acaacaagca aaaacccaaa ttaatgcaat
    20701 gattaattca aaaacaaatt atgatgttgt attcactagt ggtgcaactg aatccaataa
    20761 tcttgcttta aaaggtattg cctatcgtaa atttgataca gcgaaggaaa taattacatc
    20821 cgtgttagag catccgtccg tattagaggt tgtaagatat ttggaagcac acgaaggatt
    20881 taaagttaaa tatgttgatg taaagaaaga tggcagtatt aacttagaac acttcaaaga
    20941 attaatgtca gacaaagtcg gtttagtaac atgtatgtat gtaaataatg taactggaca
    21001 aatacagcct attccacaaa tggctaaagt tataaaaaat tatcctaagg cacattttca
    21061 tgtagatgcg gttcaagcat tcggcaaaat ttcaatggat ctcaataaca tagatagtat
    21121 tagtttaagt ggacacaagt ttaatggttt aaaaggacaa ggcgtcttac ttgtaaatca
    21181 cattcaaaat gttgaaccaa ctgtccatgg tggtggtcaa gaatatggcg ttagaagtgg
    21241 aacagttaat ttgccaaatg atattgcaat ggttaaagcg atgaagatag ctaatgaaaa
    21301 ctttgaagca ttgaatgcat ttgttactga gttaaataat gacgtccgtc aatttttaaa
    21361 taaatatcat ggagtttata ttaattcttc aacttcaggt tcaccattcg ttttaaatat
    21421 tagttttcct ggcgtaaaag gtgaagtatt agttaatgct ttttcaaaat atgacattat
    21481 gatatctacg acaagtgctt gttcatctaa acgtaataaa ttaaatgaag tattggctgc
    21541 aatgggatta tcagacaaat ctattgaagg tagtataaga ttatcatttg gggctactac
    21601 aactaaagaa gatatagcga ggtttaaaga aatatttatc atcatttatg aggaaattaa
    21661 ggagttgcta aaataatgaa gtatgatcac ttgcttgtta gatacgggga gttaacatta
    21721 aagggttcaa atagaaagaa atttgtaaat caattaagaa ataatgtaaa taagtcatta
    21781 aaaggacttg atgggtttgt cgttaaaggc aaacgagatc gtatgtatat tgaacttgaa
    21841 gaccatgcag atataaatga aataacatat cgattatcaa aaattttcgg tattaaatct
    21901 attagtccag tattaaaagt agaaaaaaca atagaggcaa taagtgcagc ggcaattaaa
    21961 tttgcgcagc aatttgaaga aaacagcaca tttaaaattg atgtgaagcg tgccgataaa
    22021 aatttcccaa tggatacgta tgaattacag cgtgaattgg gtggtgcagt attgaagcac
    22081 ttcgacaata tttcagtgaa tgtcaaacgt ccagatcatg aaattcgagt ggaagttaga
    22141 ttagatgcaa tttatatgta tgaagaagtt gttccgggtt caggtggatt accagttggt
    22201 actggtggta agacgttact aatgctttca ggcggtatag actcaccagt tgctgggatg
    22261 gaagtgatga gacgtggcgt aacaattgaa gcgattcatt tccatagtcc accatttaca
    22321 agtgatcaag caaaagaaaa agttattgaa ttgacacgta ttttagctga acgtgttgga
    22381 ccaattaaat tgcatattgt accatttaca gaattgcaaa aacaggtaaa taaagttgta
    22441 catccaagat atacaatgac ttcaacgaga cgtatgatga tgcgtgttgc tgataaatta
    22501 gtacatcaaa taggggcttt agctattgta aatggtgaaa acctagggca ggtagccagt
    22561 caaacacttc atagcatgta tgcaattaat aatgtaactt ctactcctgt attacgtcct
    22621 ttattaactt acgataaaga agaaattatt attaaatcga aagaaattgg tacatttgaa
    22681 acatctattc aaccatttga agattgttgt acaattttca cccctaaaaa tccagtaacc
    22741 gaaccaaact ttgataaggt agtccaatat gaaagtgtct ttgattttga agagatgatt
    22801 aatcgtgctg ttgaaaatat tgaaacactt gaaataacta gtgattataa aactattaaa
    22861 gaacagcaaa caaaccaatt aataaacgac tttttataaa taaaatccta gagtaaattt
    22921 aaacataagg ggatgttaaa ctatggattt gaacttaacg atgattataa tcataatttt
    22981 atttggtttt atcgcggcgt ttatagattc ggttgtaggg ggtggcggtt taatttctac
    23041 gccagcatta ttagcaatcg gtctaccacc atctgtggct ttaggtacaa ataaattggc
    23101 aagttcgttt ggttctttaa ctagtacgat aaagtttata aggtccggta aagtggactt
    23161 atatgttgtt gccaaattat ttggttttgt atttttggca tctgcatgtg gcgcatatat
    23221 tgcaacgatg gttccgtcac aaatattgaa acctttaatc atcattgcac tttcgtcggt
    23281 gtttatattc acattactta aaaaagattg gggcaataca cgcacgttta ctcaatttac
    23341 atttaagaaa gccataatat ttgcagcact ttttatatta atcggctttt atgatggatt
    23401 tgtaggtggg ggaacaggtt catttatgct ttttgtattg ttagtctttg gttttgattt
    23461 tttaagtgca gcaggaaatg ctaaagtttt gaactttgct tctaatatag gtgcgcttgt
    23521 attatttatg gtattaggac aagtagatta tgtaataggt ttaattatgg ctataagtat
    23581 gattgctggg tcatatgctg gggctcattt tgctattaaa caaggtgttg gttatgtaaa
    23641 agtattattt attattatta ctgctatttt aattttaaaa aatgcttttg attatattca
    23701 acaatttgta taaaattaaa tttgcttttg attaaagcat gaaaattgta atcaaaccat
    23761 aaattgtcgt atgatgtagt tagaatttta aaatgcagga ggtcaagtat atgactgaaa
    23821 taacattcaa aggtggacca atccacttaa aaggtcaaca aattaatgaa ggtgattttg
    23881 cacctgattt tacagtgtta gataatgact taaatcaagt aacattagca gattatgctg
    23941 gtaaaaagaa attaattagt gtggtaccat caattgatac aggtgtttgt gatcagcaga
    24001 ctcgcaaatt caactctgat gcttctaaag aagaggggat tgtgcttaca atttcagcag
    24061 acttaccatt cgcacaaaaa agatggtgcg cttcagcagg tttagacaat gtcattacat
    24121 taagtgacca ccgtgactta tcatttggtg aaaactatgg cgttgttatg gaagaacttc
    24181 gcttattagc tcgtgcagta tttgtattag atgcagataa taaagttgtt tataaagaaa
    24241 tcgttagtga aggtactgat ttcccagatt ttgatgctgc tttagctgca tacaaaaata
    24301 tttaatcatt aaagagataa atcttaaaat gtatacatcg tgtccatcgt tgtcaacagc
    24361 attaaaatag aattgttttc tatgattgct aagacctatg ggcacttttt attggagagg
    24421 gacgaatatg gcagaacaac aaacaattat ggaacgcttg tttcatacat tagatgaaaa
    24481 agctaaaaca ttaaataatg aaaatggcca aagttttatt gaaaatcttg ggctagcaat
    24541 ggaacaagta tataccaatg aaagaggatt gttagaacaa tcgacgttac aagatcgacg
    24601 taaagcattc caatttgcat atttaagttt aatgcaggaa gaaaagatac aagcaaatca
    24661 tcaaattaca ccagattcaa ttggattgat actaggattt ttagttgagc gttttatgaa
    24721 caaccaagaa gaattacata ttgttgatat tgcaagtggt gccggtcatt taagtgctac
    24781 tgtaaaagaa gtgttacctg aaattgcggt tatgcatcat ttaattgaag ttgatccagt
    24841 tttatcacgt gttagtgtac atttagcaaa cttcttagaa attcctttcg atgtgtatcc
    24901 tcaagatgcc atcatgccac taccattaga agaagcagat atcgttattg gtgattttcc
    24961 agtaggctat tatccaattg atgaaagaag taaggagttt aagctaggtt ttgaagaagg
    25021 acatagttat tcacattatt tattaataga acaagcaata aatgcattaa aagatgctgg
    25081 atatgccttt ctagtggtac caagtaatat ttttacaggt gaacatgtaa aacagcttga
    25141 aaaatatatt gcaacagaga cagagatgca agcattttta aatttaccac caactttatt
    25201 taaaaatgaa aaagcgcgaa aatctatatt aattttacaa aagaaaaaat cgggtgaaac
    25261 aaagccagtt gaagtattat tggcaaatat tcctgatttc aaaattcctt cacaatttca
    25321 aggatttatg acagagttaa atcagtggat ggacacaaat cgtcctaaaa aataatctgt
    25381 aatagtatag tcataaaact gtatgaatga taaaatgaaa aatgaaatag tataaaaagc
    25441 aaacgatgga ggatcattac ttatgtcaaa attaatcttg gctatcaatg ctggtagttc
    25501 atcattaaaa tttcaattaa ttagaatgcc tgaagaggaa ttagtaacaa aaggtttagt
    25561 agaaagaatc ggattgaagg attcaatttt tacaattgaa gtcaacgggg aaaaagttaa
    25621 aacagtacaa gatatcaagg atcacgttga ggcagttgat atcatgttgg atgcttttaa
    25681 agcgcacaat attattaatg atattaacga tatcgatgga acaggtcacc gtgttgtaca
    25741 tggtggtgaa aaattccctg aatcagtagc tattactgat gaggttgaaa aggaaattga
    25801 agaattaagc gaattagcac cgcttcacaa cccagctaac ttgatgggta ttcgtgcttt
    25861 ccgtaaatta ttaccaaata tccctcatgt ggcaattttt gatacagcat tccatcaaac
    25921 aatgcctgaa aaagcatatc tatatagctt gccatatcat tattataaag attatggcat
    25981 tcgtaagtat ggtttccatg gtacaagcca taaatttgta tcacaaagag cggcagaaat
    26041 gttagataag ccaattgaag atttacgtat tatttcatgt cacattggta atggagcatc
    26101 aattgcagct atcgatggcg gtaaatctat tgatacatca atgggcttca caccacttgc
    26161 tggtgtaaca atgggtactc gttctggtaa tattgaccct gctttaattc catttattat
    26221 ggaaaaaaca ggtaaaactg ctgaacaagt attggaaatc ttaaacaaag aatcaggttt
    26281 attaggatta tcaggtacat ctagtgattt acgtgactta tcagaagaag ctgaatcagg
    26341 taaagcacgt tcacaaatgg ctttagatgt atttgcttct aaaattcaca aatatattgg
    26401 atcatatgct gcgagaatgc atggtgttga tgttatcgta tttacagcag gtatcggtga
    26461 aaactcagta gaaattcgtg cgaaagttct tgaaggctta gaatttatgg gtgtttactg
    26521 ggatcctaag aaaaatgaaa acttactacg tggtaaagaa ggatttatta actatcctca
    26581 ttcaccagtt aaagttgtcg ttattccaac agatgaagaa agtatgattg cccgtgatgt
    26641 tatgacattc ggtggtttaa aataattttt aaataaatat taagcgtcta tctccgctac
    26701 aattatatgt aggaggtaga cgctttttgt attgtttgaa aattaactag tgtaatgtct
    26761 cttttcacga atatcttaaa gaattaagta gtcgaagaag attatgaaaa cattattaag
    26821 tgagttttat ttataaacaa taaaaaacca agtgcagaaa gttgcacttg gttcataatg
    26881 atcaagtagt tatctaatga tcatctatat ttaattttga tatttttcac gtaattgagt
    26941 tgttgcaact tgtggttgga agtctgctgg taactcttca gtacgaacaa ctaacacgtc
    27001 acatggcgca tgacgaacga tagattctga tacagaacca acaataaatc tttccacggc
    27061 atttaagcct gatgtaccac tcataatcaa gtctgcatta atttcatgtg caagcttttt
    27121 aggaatgata gatttaggag agccaaactc tagacgcgtt tctacatctt taacaccagc
    27181 gttagtagct acttctttat aaccatttaa taattcttct gcaaaatgct tagatttttc
    27241 agtaaattga gcatcataaa cttcataaga agaatacgtt cttgaatcaa ttacattcac
    27301 aattgttaac ttcgcatcgt tacgtttagc aacaccaact gctctgttaa atgcccattc
    27361 cgcttcatgt gaaccgtcaa ctgcgattaa aatatttttg taagtaatca tagtaagtgc
    27421 ctccttgttc tttcttaatt atattttacc acaattttcg gaaaagtcag tgtaataacc
    27481 tttacaattt tgtggatttt tgattgcgct ttcaaaatgt tcgcgttaag atgattatgg
    27541 aggtggatgg tatgaaaatt ggtataccaa gggagattaa aaataatgaa aatcgtgttg
    27601 gtttatcacc aagtggtgtg cacgctttag tagaaagtgg gcatactgtt ttagtggaaa
    27661 caaatgcggg ttcaggatca ttctttgaag atgtagatta caaagaagca ggtgctgaga
    27721 ttgttgctga acaagcaaaa gtttgggatg tggatatggt tattaaagtt aaagaaccac
    27781 ttgaatctga atatccatat tttaaagaag ggcttgtatt attcacttat cttcatttag
    27841 caaatgaaga aaaattaaca caagctttga tagatagaaa agtaattagt attgcatatg
    27901 agactgtgca gttaccagac cgatctttac cattgttatc accaatgagt gaggtagcag
    27961 gaagaatgtc agctcaagtt ggcgcagagt tcctacaaaa acttaatggt ggtatgggaa
    28021 ttctactagg tggtgtccca ggagtaccta agggtaaagt aactattatc ggtggtggtc
    28081 aagcaggaac aaatgcagct aaaattgcac taggactagg tgcagatgtt acgattttag
    28141 atgttaatcc aaagcgttta caacaattag atgatttatt cggtggacgt gtacatacaa
    28201 ttatgtcaaa tccgttgaat attgagttgt atgttaaaca aagtgattta gtaataggtg
    28261 cagttttaat tccaggtgct aaagcgccaa gacttgtaac agaagacatg attaaacaaa
    28321 tgaaaaatgg gtcagttatt attgacattg ctattgatca aggcggtatt tttgaaacaa
    28381 ctgataaaat tacgacacat gatgatccta catatattaa gcatggtgtg gttcattatg
    28441 cagttgcaaa tatgccaggt gcagtaccgc gtacttcgac gttagcttta aataatgcta
    28501 cgctacctta tgcgctcatg ctagctaata aagggtatag agaagcattt aaatcaaatc
    28561 aaccattatc attaggttta aatacttaca aaggtcacgt aaccaataaa ggcgttgcag
    28621 aggcatttga aatggaatat aaatctgtag aagaagcatt acaattataa tttgaatttg
    28681 atgaaaaggg aatggattac agcgtactaa agaatgacgt tatcatctcc ttggttatta
    28741 aaattaaata atgataaatt aaagctagaa acaatgcgaa gaaatcatcg aacatgaatg
    28801 atattgattt gattcattac atgttactga tgaacatacc tgcatatgtg tttctagctt
    28861 gtttgttaca tattgcattt agacttggat aaaaatttat gtcttaggcg ctacaatgtt
    28921 atattggcag tgtttgactg aatgagaatg cgcttgtaac aagcttttca tttttatccc
    28981 actccttatt tttcgtaatg tgttaatact tcatatcctt cattagtgac aagtatgtca
    29041 tcttcaattc ttacacctgc aacaccaggt acataaatac ctggttcgat tgtaataacc
    29101 atgccagctt ctaacaaatt agaattagta cttgaaacat cttgatattc atgttcttgt
    29161 aatcctaggc catgacctaa gcgatgaggg aaatattcac cataaccttt ttctgaaata
    29221 atatttctag cgatatgatc gatatctttt aatggtattc caggtttaat tgcttggatt
    29281 gcagatgttt ctgcttctaa tacaatatta taaatttctt gtgcttcttt gctaggttca
    29341 ccaaatttaa tagtacgtgt catatcgcta caataatgct cataaattac acctaaatca
    29401 aatagtacat attcattgct ttttaatctg cgatctcctg gtgtgccatg aggtgatgcg
    29461 gcatgatctc caaataaaac catcgtatca aaactcattt cattgacgcc atattgtttg
    29521 atagtttgct caatatggtt gactacttca cattcagtca caccttcttt taaataagaa
    29581 acacctattt cgatacactt atctgctaac tcagcagctt tacgtatttt gctaatttca
    29641 tcttcggatt taatatttct caattgtttg attgttaaat caacatctcc gaatgaattg
    29701 acattgaaac cagagattaa ttgtttttgg cgtgctactg ttaagtgctc gctttcaatt
    29761 agtaatttat tgattgtttg aggataaagt gaaaaagggt tttcagtgtc taaatatcca
    29821 acgatttcac ctgtgaaagg tgatgctttg acttcttcga cttccatttt tggacaaaat
    29881 agtacttgtt taccatcttt cttaatcaat aatgcaaata atctttcatg gggttcgcta
    29941 cggtatccag taaaataata tacattcaac ggtgttgtaa tccatgctgc atcagcttgt
    30001 tgattgttca attcgtctat tatttttgat atttttgtca ttttataaac actcctcaac
    30061 ataatattac ttccatttta tgccaattat tattagattt caaaatcgag ctaatacatt
    30121 aactagattt tcaattttta aaatttgaga atttgttata attaagtggc aaaagggtac
    30181 taaatttata tagtaattga taggaggatg ctcaaatgaa gttatcattt catggtcaat
    30241 caacgattta tttagaaggg aacaacaaaa aggttatcgt agatccgttt atttcaaata
    30301 accctaagtg tgatttgaat attgaaacag tgcaggtaga ctatatcgtt ttaacgcacg
    30361 gtcattttga tcattttggt gatgttgtgg aactagcaaa gaaaacagga gcaacagtaa
    30421 ttggaagtgc agaaatggct gactatcttt cttcatatca cggtgttgaa aatgttcatg
    30481 gtatgaatat tggtggtaaa gcaaactttg atttcggaag cgttaaattt gttcaagcat
    30541 ttcatagttc aagttttaca catgaaaatg gtattccagt ttatttaggt atgccaatgg
    30601 gtatcgtgtt tgaagttgaa ggaaaaacaa tttatcatac tggtgataca ggtctgttta
    30661 gtgacatgtc acttattgcg aaacgtcatc cagttgatgt atgttttgta ccaattggag
    30721 ataattttac gatgggtatt gatgatgcaa gttatgctat taatgaattt attaaaccaa
    30781 aaatttctgt accgattcat tatgatacat tcccattaat cgaacaagat ccacaacaat
    30841 ttaaagatgc tgttaatgta ggggacgtgc aaattttaaa accaggtgaa tctgttcagt
    30901 tttaaataga atatagattt tacactaaaa aacacctcgc taatctatgt ggaagttatc
    30961 gtctgcatat ttttagtggg tgtttttata ttctactgat tcagttacga cgtggacggg
    31021 ggacatcagt ggttctttat catttctgac ctactctcgt aattttagtt ttatacaatt
    31081 agacattgat tataaattta ctacgatata aaaaatccag aaccacagtc ataatcgttt
    31141 cttgtattta ctcagtcgtt tgagactgca tcatgattga aagattattt gtgattctgg
    31201 ataaaaattt tatttaacga ttaatacagg gatagtcgca cgttttgcta ctttgtggct
    31261 gacacttcca agtacaaatt tctttttgtc ttctgcttta cggttactta aaacaacaat
    31321 ttcgtattta ccgctattag catgttttaa tagttcttct tttgcattac cacgaacaat
    31381 tatttggtca taatcaatac cataatcttc taaagctttg cgtgtttttt ccaatcgctt
    31441 gcttcgttct tcagtaagtt tgtttaaatg aacacctgct ttaattgatg cttgagcatc
    31501 ttgttcgctg attgcgttta aaactgttac gactgtacct tcgccagcta atttagacac
    31561 ttcttttagt gctttttcat tttttaactg agtgtctaca ccaagtaata tatttttata
    31621 cattagtaca tccctcctca cttacattct aataaataat aatggatttt tccatttaaa
    31681 attaaagatt ttgtgatctt tgtgtttgtt gtatattttc aaaaaataaa ataggtgaaa
    31741 cgatcattat ttcatttcac ttcaatgctt tttatccaaa ataaagttat aatgtagtta
    31801 atactgttcg aaaagaagag gtaaagaatg acaaaacatg aacaaatttt agattatatt
    31861 gaatcgttat ctataggctc taaaatttcg gtgcgtaaaa tcgctaaatt tttaaacgta
    31921 tctgaaggga cagcatatcg tgcaattaaa gatgctgata aaatgggcat ggtcgcaacg
    31981 atagatcgtg ttggtacggt tcgaatcgaa aaacgtaatc gtaatgaaat tgaacattta
    32041 acttttaatg aaattgtcaa tattattgat ggccaagtgt taggtggcaa caaaggtatc
    32101 actaagatgg tatctaaatt tgcaattgga gcaatggaac tcaaagatat tttaaagtat
    32161 attggtccaa agacattatt gattgtcgga aatcgtgaag atgtgcagat tgaagcttta
    32221 aaaagaggta cggctatatt aattactggt ggatttaagc catctaataa agttattaat
    32281 tttgcgaatg aacatgattt acctgttcta tcatcaagtt atgatacatt cctagttgca
    32341 aatattatca acaaagcact ttttaatcag aaaatacgaa aagatatctt aatagttcaa
    32401 gatataatga cgccattaga tgatttatct gtgctgtttg atacgatgaa aatagcagat
    32461 tataagcgta tggcgaatcg aactggccat acaagatttc cggtagtcaa tgagtcgtat
    32521 aaattagtag gtattgtaac aagtcgagag atgattaata caaaggacga cgacgaaata
    32581 gataaagtga tgacgcgaaa tcctatttat gtaaatgcta tgagtacagt agcaagttgt
    32641 gcgcatatga tgatttggga aggtattgaa ttgattcctg tcgtttcgag taataaaaag
    32701 acagtgggtg tgattaatag acaagatgtt ttaaagtcta tgcaactttt aggaagacag
    32761 cctcagatgg gtgaaacaat taacgatcaa atcgccaaat atataacaat gaatcaagat
    32821 ggaattacag tagaagtttc accactttta atcaatcatt acggaacagt aagtaaagcg
    32881 gcatttgtaa gtattataga agaaacaata caatatgaaa tgcgtaaatt taaaaaaggt
    32941 aatgtcatga tagagaactt aaatatcgtg tatattaaaa cagtacctat tgaatcacat
    33001 ataacagtgc gctttggcat actagacgtc ggaagaaact tcgccaaaat agaagtgaac
    33061 atgcatagtc aaaacgacaa agtagcgagc gctctcgtga tatgccaaat gtttgacgaa
    33121 gtataggaac ttagcagaga aaataggcat aagatagatt tcgcacaaac gttggggccc
    33181 caccccaact tgcattgttt gtagaaatta ggggtccaat ttctctgtgt tggggcccct
    33241 gaactaaata gtatgaaaat atatatatct aaataattaa aacgggcaaa tagtttaata
    33301 tggtagtaac aatttttata atttagatat tttgatatag actaatgctt aaattaagca
    33361 acttttctaa aaaagggagt atgaaaatga ttagtacttt gaatgaaatt atgaaatgta
    33421 tcgaagacaa cgatacaatt attatacaca gacatgttag accagatcct gatgcatatg
    33481 gttcacaact tggtttaaaa tactatattc agcagaaatt tccgcaaaag caagtatttg
    33541 cagtgggtga agcggaatca tcattaagtt ttattggaga gttagataac attgatgata
    33601 aaacatatca agatgcgctt gtaattgtat gtgatactgc caatgctcca cgaattgacg
    33661 atgaacgtta tagtacaggt cgtaaactta ttaaaataga tcatcatcct gcagttgatc
    33721 agtatggtga tattaattta gttaatacga acgcgtcatc tacaagtgaa atcatttacg
    33781 atttaatctc acattttaat gatgaagcaa ttgttaataa agatatcgcg agcgttttat
    33841 atcttggtat cgtcggtgat actgggcgat tcctttttaa caatacctca gaacatacta
    33901 tggaaattgc tggaaagtta attgggcatg atattgatca taatgcgctt ttaaataaaa
    33961 tgatggagaa ggacccaaaa atgttgccgt ttcaaggtta tgttttacaa catttcgaac
    34021 ttatggatga tggattctgc caagttaaaa taactgaaga tgtattggag caattcggta
    34081 ttcagccaaa tgaagcatct cagtttgtta atacaattgc tgacatcaaa ggcttgaaaa
    34141 tatgggtatt tgcagtcgat gaaggtaatg aaatcagatg tcgattacgt tctaaagggc
    34201 aattgattat taatgatatt gcgcaagatt ttggtggcgg tggtcatccg aatgcgtcag
    34261 gagtttcagt ggacagctgg gatgaatttg agcaacttgc tacagcttta cgcacaaaac
    34321 ttaactaata gaaaggggcc attcaatcat ggtggcatat ttaaatattc atacggctta
    34381 tgatttgtta aattcaagct taaaaataga agatgccgta agacttgctg tgtctgaaaa
    34441 tgttgatgca cttgccataa ctgacaccaa tgtattgtat ggttttccta aattttatga
    34501 tgcatgtata gcaaataaca ttaaaccgat ttttggtatg acaatatatg tgacaaatgg
    34561 attaaataca gtcgaaacag ttgttctagc taaaaataat gatggattaa aagatttgta
    34621 tcaactatca tcggaaataa aaatgaatgc attagaacat gtgtcgtttg aattattaaa
    34681 acgattttct aacaatatga ttatcatttt taaaaaagtc ggtgatcaac atcgtgatat
    34741 tgtacaagtg tttgaaaccc ataatgacac atatatggac caccttagta tttcgattca
    34801 aggtagaaaa catgtttgga ttcaaaatgt ttgttaccaa acacgtcaag atgccgatac
    34861 gatttctgca ttagcagcta ttagagacaa tacaaaatta gacttaattc atgatcaaga
    34921 agattttggt gcacattttt taactgaaaa ggaaattaat caattagata ttaaccaaga
    34981 atatttaacg caggttgatg ttatagctca aaagtgtgat gcagaattaa aatatcatca
    35041 atctctactt cctcaatatg agacacctaa tgatgaatca gctaaaaaat atttgtggcg
    35101 tgtcttagtt acacaattga aaaaattaga acttaattat gacgtctatt tagagcgatt
    35161 gaaatatgag tataaagtta ttactaatat gggttttgaa gattatttct taatagtaag
    35221 tgatttaatc cattatgcga aaacgaatga tgtgatggta ggtcctggtc gtggttcttc
    35281 agctggctca ctggtcagtt atttattggg aattacaacg attgatccta ttaaattcaa
    35341 tctattattt gaacgttttt taaacccaga acgtgtaaca atgcctgata ttgatattga
    35401 ctttgaagat acacgccgag aaagggtcat tcagtacgtc caagaaaaat atggcgagct
    35461 acatgtatct ggaattgtga ctttcggtca tctgcttgca agagcagttg ctagagatgt
    35521 tggaagaatt atggggtttg atgaagttac attaaatgaa atttcaagtt taatcccaca
    35581 taaattagga attacacttg atgaagcata tcaaattgac gattttaaaa agtttgtaca
    35641 tcgaaaccat cgacatgaac gctggttcag tatttgtaaa aagttagaag gtttaccaag
    35701 acatacatct acacatgcgg caggaattat tattaatgac catccattat atgaatatgc
    35761 ccctttaacg aaaggggata caggattatt aacgcaatgg acaatgactg aagccgaacg
    35821 tattgggtta ttaaaaatag attttctagg gttgagaaac ttatcgatta ttcatcaaat
    35881 cttaacacaa gtcaaaaaag atttaggtat taatattgat atcgaaaaga ttccgtttga
    35941 tgatcaaaaa gtgtttgaat tgttgtcgca aggagatacg actggcatat tccaattaga
    36001 gtctgacggt gtaagaagtg tattaaaaaa attaaagccg gaacactttg aagatattgt
    36061 tgctgtaact tctttgtata gaccaggtcc aatggaagaa attccaactt acattacaag
    36121 aagacatgat ccaagcaaag ttcaatattt acatccgcat ttagaaccta tattaaaaaa
    36181 tacttacggt gttattattt atcaagagca aattatgcaa atagcgagca catttgcaaa
    36241 cttcagttat ggtgaagcgg atattttaag aagagcaatg agtaaaaaaa atagagctgt
    36301 tcttgaaagt gagcgtcaac attttataga aggtgcaaag caaaatggtt atcacgaaga
    36361 cattagtaag caaatatttg atttgattct gaaatttgct gattatggtt ttcctagagc
    36421 acatgctgtc agctattcta aaattgcata cattatgagc tttttaaaag tccattatcc
    36481 aaattatttt tacgcaaata ttttaagtaa tgttattgga agtgagaaga aaactgctca
    36541 aatgatagaa gaagcaaaaa aacaaggtat cactatattg ccaccgaaca ttaacgaaag
    36601 tcattggttt tataaacctt cccaagaagg catttattta tcaattggta caattaaagg
    36661 tgttggttat caaagtgtga aagtgattgt tgatgaacgt tatcagaacg gcaaatttaa
    36721 agatttcttt gattttgcta gacgtatacc gaagagagtc aaaacgagaa agttacttga
    36781 agcactgatt ttagtgggag cgtttgatgc ttttggtaaa acacgttcaa cgttgttgca
    36841 agctattgat caagtgttgg atggcgattt aaacattgaa caagatggtt ttttatttga
    36901 tattttaacg ccaaaacaga tgtatgaaga taaagaagaa ttgcctgatg cacttattag
    36961 tcagtacgaa aaagaatatt taggatttta tgtttcgcaa cacccagtag ataaaaagtt
    37021 tgttgccaaa caatatttaa cgatatttaa attgagtaac gcgcagaatt ataaacctat
    37081 attagtacag tttgataaag ttaaacaaat tcgaactaaa aatggtcaaa atatggcatt
    37141 cgtcacatta aatgatggca ttgaaacttt agatggtgtg attttcccta atcagtttaa
    37201 aaagtacgaa gagttgttat cacataatga cttgtttata gttagcggga aatttgacca
    37261 tagaaagcaa caacgtcaac taattataaa tgagattcag acattagcca cttttgaaga
    37321 acaaaaatta gcatttgcca aacaaattat aattagaaat aaatcacaaa tagatatgtt
    37381 tgaagagatg attaaagcta cgaaagagaa tgctaatgat gttgtgttat ccttttatga
    37441 tgaaacgatt aaacaaatga ctactttagg ctatattaat caaaaagata gtatgtttaa
    37501 taattttata caatccttta accctagtga tattaggctt atataactt
//
LOCUS       DAKTIO010000008        37068 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.008, whole genome shotgun sequence.
ACCESSION   DAKTIO010000008 DAKTIO010000000
VERSION     DAKTIO010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 37068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37068
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.008"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(33..329)
                     /locus_tag="O0293_000275"
     CDS             complement(33..329)
                     /locus_tag="O0293_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279188.1"
                     /translation="MFNGLNEKIVALINDAQPLIITLLALSIFVSGAVMMFGDTGVRW
                     AKRTLIGAIIGAVLSGGALVYARSFKKIVNVSAVFPSATETKFAVIDTLQIIFM"
     gene            complement(333..527)
                     /locus_tag="O0293_000276"
     CDS             complement(333..527)
                     /locus_tag="O0293_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279189.1"
                     /translation="MSKITQNDLNQLKKQVTEIMKVTGETHNEWLYNQYQNYLSENTD
                     VVMEALRFYSENKNNTAWEE"
     gene            complement(789..1079)
                     /locus_tag="O0293_000277"
     CDS             complement(789..1079)
                     /locus_tag="O0293_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279190.1"
                     /translation="MLCYKKNKRFDFIAFYFFADEIRTFDGKTYRIKGMRCQRIDHKR
                     LDINERKTLRHLSEVIYKTKTVFNDNYGLIAYRKKVSQELKSVKNISISKSV"
     gene            1497..1817
                     /locus_tag="O0293_000278"
     CDS             1497..1817
                     /locus_tag="O0293_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279191.1"
                     /translation="MQKYPRVFRIKSTTKQLIKITSAINDLNNSDFVEKIIQETELSN
                     IDFSSYNNYGKKSYNTVQATYTISYSSNEKIEAVAQKHGLTRSQVLNLFLSKYLNIPI
                     DHKD"
     gene            complement(1911..2207)
                     /locus_tag="O0293_000279"
     CDS             complement(1911..2207)
                     /locus_tag="O0293_000279"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279192.1"
                     /translation="MRNKNYIGGLTKENLKGFVILVLIFLYVLSALLNLTGFFWGYVF
                     IPVFAIWSIYMMFFRGYFIPVVKIFFIITLTTIKFVFMIFFIMAIASSLSDKKY"
     gene            complement(2252..3241)
                     /locus_tag="O0293_000280"
     CDS             complement(2252..3241)
                     /locus_tag="O0293_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCY0279193.1"
                     /translation="MSEQRFNIQQQYREKFYQLPKVFFTNEKYMQLSNDAKIAYALLK
                     DRLELSIKNNWFDENGDIFFIFTNENLKTILNCHDGKLTKIKKELSKADLLEQVRCGQ
                     GKPNKLYLKNPTITKEDVYELKKQEETSVEPSNDAEMRFSHFLKCENRISRNAEIAQQ
                     EMRKSHTNDTDLSNTEYIDTDNNDMNDLNDIEFKNKISNHSNHSNHDSLNSDEEALKF
                     TELQEFPEQSKHYLNNFSYRELKVVKEIILKAKKSFNTEHNTTYMLEDIDYDLVNVLK
                     RFKAKVYMKNESVETMQGYLMKCIKMELEELHSLNMRKKNFKNSSWNIFSQND"
     gene            3901..4767
                     /locus_tag="O0293_000281"
     CDS             3901..4767
                     /locus_tag="O0293_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002487763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCY0279194.1"
                     /translation="MDFPSKFWSNNKTENELLNKIKNKDEAIVLTINMFKGGVGKSTL
                     TNMFGYIANRYDLSVLFIDTDPQRTLTKKLSKNFSTKKEANLSFMDGIKTGTLEDSIS
                     TLSNNIDLIKGDWNLAWFDRYSRRSLNVKDEFYVYSSLIGKLKKNYDFIFFDSVPTTT
                     TLTHNCIVASDYVIVPVESEEDCYNNSIDYMFYLSQMKQYNSKLDVLGILIYLTEEDN
                     KTNNEYVKKYRNEYNDIVLKNVITRSRRVMTWGSQGISEHKPYDKKTLKKYLNVFWEF
                     MYRLDERGAFNE"
     gene            4760..4981
                     /locus_tag="O0293_000282"
     CDS             4760..4981
                     /locus_tag="O0293_000282"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279195.1"
                     /translation="MSSNLDKYSAKKRETNEIKETPFDKNIKKKEDFKLVKIDPKIHV
                     DLKFAALKEDTTMKAVVDKAIKEYLEKYH"
     gene            5293..5505
                     /locus_tag="O0293_000283"
     CDS             5293..5505
                     /locus_tag="O0293_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279196.1"
                     /translation="MENKTHYFEAHGKDYKLEVAKDMFGCEGVTVVENGLYMGMIDCT
                     DERDYKRIESMIRADKHFVYTDEVYC"
     gene            5613..5900
                     /locus_tag="O0293_000284"
     CDS             5613..5900
                     /locus_tag="O0293_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279197.1"
                     /translation="MYVAKEENGQILSESPFISCVIQDVIEQRIENFERYYNHYKENL
                     ELNYYIYIGNLNKLYNSIEDYTNELSLIDDFYKAYNDESFVHKSQIVRIET"
     gene            5975..6157
                     /locus_tag="O0293_000285"
     CDS             5975..6157
                     /locus_tag="O0293_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279198.1"
                     /translation="MNDNKKKYESQTNRRNVKVYKKTDEIASELKFELRVKTKQEVYD
                     IAIEKLYEQITGKKYE"
     gene            6196..7146
                     /locus_tag="O0293_000286"
     CDS             6196..7146
                     /locus_tag="O0293_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HCY0279199.1"
                     /translation="MNKKLIASGTIASILLLNTGGVAFADSNKLNEDIFIKGADLNSQ
                     QLEETKKKLDVDDGYKEISITTNDVAKYTGNNNLSFIHSSATITPKKFKKGVTVDIET
                     PENITRITKEQYTNAAITAGIQDSEIKVASIDKVSGEGALTGIYKAYEENGNSLNKDD
                     VQNANNEMNDLASISEQNKGQKGYSDEALNNAIAEMKKEIASEKQDGKNLTREDISKI
                     VNDILKENGLNKILSDNQINVINNIMVNVSNSEILNKDPKAYEKQAKDLASNIKDKAG
                     NLLDKAKELNTEENRNFLQKIWDSIVEFFKKIINWLLSWF"
     gene            7274..7423
                     /locus_tag="O0293_000287"
     CDS             7274..7423
                     /locus_tag="O0293_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279200.1"
                     /translation="MNKETLIDLIDMMIGLTEIERKRLSEMEMRKVEIRYKMALTEKT
                     DEMIG"
     gene            7456..7905
                     /locus_tag="O0293_000288"
     CDS             7456..7905
                     /locus_tag="O0293_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HCY0279201.1"
                     /translation="MKKIYIENIDDYILVDDEDFERVNFYKWHKSYAHDTQRFLAQIL
                     GKKTALPVFILNNQYAYQKVKNNDFTRGNLGADKHSFRYRAPQKNSSSKYKGVSYDSK
                     HNKWVASIYAEGEKKYLGRFVDEKECAKAYNKAVQKYWGGDGYLNDV"
     gene            complement(8334..8750)
                     /locus_tag="O0293_000289"
     CDS             complement(8334..8750)
                     /locus_tag="O0293_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HCY0279202.1"
                     /translation="MYVINPELPMQYKYETDYRKIPKELLNSNIPKGRDKVKWSPFKT
                     VSDQYKLLDEYIEEQNKVDMPLLSDDQLQILNETVYYNSMNNILTEISYWDNGYINSM
                     ECYINKVDLLERVMVVTSKHRGTKIKISLNYIVSVE"
     gene            8825..9631
                     /locus_tag="O0293_000290"
     CDS             8825..9631
                     /locus_tag="O0293_000290"
                     /inference="COORDINATES: protein motif:HMM:NF014566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCY0279203.1"
                     /translation="MKNTKNWYESLFSGVYDGLKISFPLVLIYLIAYIHDSLSFNPSI
                     NVIRWSFGVIVSTLIFFLIFKFKFFAKDWKLTFTNKSFLIVIGSSILINLIGYFQNVF
                     DLYKESKNQKIIEEKLQNNQDHLHFYFVDMVIVAPIYEEIIFRGIIYFYFIKAIEKLF
                     SQKSWEINARYSVIMCNGMFIMTSSLLFAYLHAWDTYIEAIPYLSMGIILSAVVVISK
                     NIINSILIHMINNFFAFMYVETKEIFSLLIALIAVAIIMCLLELKRKRIS"
     gene            complement(9716..10705)
                     /locus_tag="O0293_000291"
     CDS             complement(9716..10705)
                     /locus_tag="O0293_000291"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279204.1"
                     /translation="MNNIDAFNSLVFTFLTIFSIVYITVNCFNPNFFKHDTRYIPLNR
                     VLKLGILNSLYLIIGIWLLSKFYSALINSHISLEKLGDDSFIIATFLTSSILISFFIL
                     SLFLVVLKTIKMLFVYINLKKDNIPNNLRHVLYKKNFETVINNYKLLKMSDEKVSLSD
                     KEILQLSAVLSNAGMNEDVQEILKIKLFKQNTFWDDMFSVFPNQANYSVQCSSEYISY
                     NEQFKTALQKHAKVFRYIYVPIIIIAIIQIYFPIFNLIINTSPTTRERTIVLVISIII
                     NIMLIIKYKTLKRIHKNFLNIANVKIKASNTDKAIFTAQCILAVTTIICLLIS"
     gene            complement(10698..11423)
                     /locus_tag="O0293_000292"
     CDS             complement(10698..11423)
                     /locus_tag="O0293_000292"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279205.1"
                     /translation="MQNNDMISKINILLQSDIQTNQIANDTGISKVTISNLRRKTADI
                     SKATFETVNKLYNYYLDRKDYLELAKTIEKDILNVKLPKDVQIFITKLKEAVDNINDI
                     TKSLHISEITLEKSFTMSKDKKSTKLISKIKLDELIPIQIKRNTFAYSLKISSDFVED
                     KTPLDNITDFQIDFSYNDLEIDLKRHIHLGNRVVLITSNLNELGESQTGIYVNGNNGG
                     YNYELNFISISIFSNDRKGDKYE"
     gene            11578..12291
                     /locus_tag="O0293_000293"
                     /pseudo
     CDS             11578..12291
                     /locus_tag="O0293_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12719..13273
                     /locus_tag="O0293_000294"
     CDS             12719..13273
                     /locus_tag="O0293_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCY0279206.1"
                     /translation="MAKIGYARVSTKDQSLDGQIDTLKEFGCERIFSEKISGRKVKRT
                     ELDKCLDYLREGDTLVIYKLDRLGRTTKQLIELSQWLDDNGIDLQIIDMNVSTKDAMG
                     KMFFTMMSAFAELEANLLRERTKKGLAAARARGRLGGRPSLPDHKKREIKFLYDEQMT
                     TGEEIAKKTGVSRSTVYRIVKERK"
     gene            13535..13900
                     /locus_tag="O0293_000295"
     CDS             13535..13900
                     /locus_tag="O0293_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279207.1"
                     /translation="MDKNKLEIYLRICENIKEYKNLEFECYNEDEEVFDSNLQCPLAY
                     TTKGDNEEFEIQVTLDLNNNQIIKEISHVYINYKEYECFKDWEEIASKTLNFDDLIMT
                     DMDVDDLLEEIPNKKGIKY"
     gene            complement(14772..15377)
                     /locus_tag="O0293_000296"
     CDS             complement(14772..15377)
                     /locus_tag="O0293_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HCY0279208.1"
                     /translation="MTEINIYNILLLIFMLVIAYKLTPVIYRFIKKKKIQALYRRAHI
                     IDIDKMEGIEFEYYLEALFLKLKYKPIVTKASRDYGADLILKKENKKIVVQAKRSNSK
                     IGIKAVQEIYTAQRFYNADEAWLVTNSFYTKSAVELGKACDVVMKDRTSLSKWIISIN
                     PDFSNNEKCPRCNHKLVVRTGKNGQFLGCSNYPNCKYTKNI"
     gene            complement(15380..16396)
                     /locus_tag="O0293_000297"
     CDS             complement(15380..16396)
                     /locus_tag="O0293_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 and TOPRIM domain-containing protein"
                     /protein_id="HCY0279209.1"
                     /translation="MSKQSYTNEEKEHARKTDIREIGEAYGLTFKKEGKYDRCIEHDS
                     LILKGQSYYWNSKGVKGHGGLSFCIDVLDMDFKEAMSILTQDGFSQYKNDNFSIKEKK
                     TLNYNTQTEMKDSTRAYNYLVNERCLDPKLVRWGFNTGVIAQDKQNNVCFKWLDNENN
                     IVGVDKRGTGRKAFKQIVEGSKQHSGFHIDIIQDKNIGIRNVVITESPIDALSYYDSH
                     RDLKQTRIASLSGLKLETFKQHILDVGKYNLKLGEEPKEYPLESIVLAVDNDEAGKNF
                     VSSIVNTYENDFTIDLPLNSKDWNEQLKKDKAITDGKTLYQKNEIKEQLKEQTHNEKD
                     DREI"
     gene            complement(16451..17800)
                     /locus_tag="O0293_000298"
     CDS             complement(16451..17800)
                     /locus_tag="O0293_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279210.1"
                     /translation="METPIKDILKEKYKIDIDKEALHELIAKQVDKELLKRMSSKSRE
                     NLGLSGYFTELQQSITGIKARLHEKFTGETINKNIHGIKAFLSDYAINPKIISENIKE
                     LLGKSKDNVVDAFSKIKTLFKEEQQNLRHTELNNEILLSEKFSDLQDMMKDSYNIINN
                     AENIKELRNYKQITRLEIIEQTLALKDNPETENKYNFNNFSKHIDWSNQDNQTQMLEL
                     YNYLKDANEAIHFSDPYSIDNDYNKLQLEQFTLQGIQKIEASINAKDLNESRLNHLEN
                     NRNTSLNFTENNLKNFNKELKGVHRSLTKESEQAIKENKVIELEREKEIYEAGKIQES
                     ELFQTLNNQNTFAVTPEKEPSELEKANFVGIEQGEIIVEVDENLYALTLNDKDKEKIQ
                     KNADIFEFTYNTQTGEFKYSENKENSVVKSNQNVISNNQNPLPKEYIELAEQENYER"
     gene            complement(17842..19959)
                     /locus_tag="O0293_000299"
     CDS             complement(17842..19959)
                     /locus_tag="O0293_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HCY0279211.1"
                     /translation="MKTVIFAEKPSQARDYANALGISHKKDGYIEGVNPMFDGGVIIT
                     WGFGHLVELKLPKDYSNPINNWDLKNLPYRPSPFEYKVSEGKSKQFNIVKSLFKDADI
                     LINATDIDREGSNIFYSTLKLTGVKNKTIKRLWIKSLVEKEIKKGFQQLQNNHQDYQN
                     YQEAYARQTSDYLIGMNLSPVYSKIFQLQGVQDVFSIGRVQTPTLYMIYERYLAIENF
                     KPETFYEIFGEFESKNGCYKGKAKIKTTDKQEIINLGEKHNLKDAKEGIITKLETQEK
                     RTSSPKLHSLSTLQSKINKQYKYSPEKTKNIVQSLYEKKILSYPRTDCNYITSEEYLY
                     LKENLQSLMNVYNKHFSIVYDKPRARYVNNEKVKEHYAIIPTSQIPTPQKIEKLSQDE
                     KYVLDEVVSTTLSMFANDYVYDETKIETNVNQLIFYTTGHTEIQKGWKILFKHKGNEK
                     SPEQQLPKLIKNECVKANIYSKKGITQSPKLYTEGQLITLMKSCGKYLDDEEAEILKE
                     IQGIGTEATRDGIIQSLKNKNYIKIEKNKVHITAKGILLCKAVKGSLLASATMTAAWE
                     KRLKLIGQGQANTDNFIDITMKFIDKEIKAYQEKCEDKSIINQSNIVAKDNEFGECPN
                     CTDGQLIDKNKIISCTNCEQVFFKNFYQKKLTDKQIQELIFNGKTSKKLKLKNKAGKT
                     YEAYLILIEDKKTGTKRYNISFD"
     gene            complement(19983..20657)
                     /locus_tag="O0293_000300"
     CDS             complement(19983..20657)
                     /locus_tag="O0293_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279212.1"
                     /translation="MMNQNEQGKIYQTYLLKEFFSDKPLFNVEALTEREQNDLQHLKE
                     NLNPNIKAVIEESVANVISPFYELDKATKGLREVENQMKNVENIEIKDYAFSKYEDII
                     YQKSNQNKENFNYINQRFQIAKIEHTEPMETQLNKCEIASFEDGKIELEVANQLYQVT
                     IPHESYLKVKEAPNRYDFMLDKSTGNIIYKEKTETIEMSNDGLEKTPRHLKKQVVDEQ
                     DNELER"
     gene            complement(20676..22001)
                     /locus_tag="O0293_000301"
     CDS             complement(20676..22001)
                     /locus_tag="O0293_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCY0279213.1"
                     /translation="MKKAELNHFIEETSEQALKKLEEFFYSPDSVEEYISFLSQFYNY
                     SPRNQLLIANQYKGAKAIAPYKKWQSLGAQVQKGEKAIKILVPSERKTFVRDIDNKKS
                     VLPIKKATKEEKALMKKGEIKINKQLVFVKGSVFDIRQTDMPEDKYPQMIQQLRGEVT
                     DYAKKIQCLNAIAEAYNIDVKESEDSIQGARGYYTETSTGDKKIILNKDNEQAENTRT
                     MIHELSHALLHGKDKIIETPTTLGMKEEEFQAEMTALIVGSYMGLDNNDDSIQYIYGY
                     AKNMSAEDKMMLMKNVQKVSSEMIKSINDKDANDIYLEVNKYPNEVMIKPNKTKNNTF
                     LRNDQLYTIQDANYFSKEMYSQFTPTLAHQENNKTNIIQGIPFSYAPQQTMLEQPTFY
                     EYVKTYIVSQDPSSKSKVDRAVEKDSQSIKHAQNKKVIDIYTSDNQIER"
     gene            complement(21998..22561)
                     /locus_tag="O0293_000302"
     CDS             complement(21998..22561)
                     /locus_tag="O0293_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001631997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279214.1"
                     /translation="MENNFLISDAEPATFVKLAGNIITVETEEFEYDFRLTDAQLEQC
                     YQKLSKDNELNFIINPDKGIIVIDNDYDVTQQSEIDNIEDYFFAIEDMPITSKTCEIV
                     KLFSVKGKHYAEIETEDNEHLTLFIKEDVYQVIKDTLQSFEKTDDIDKVYFSYSPEFH
                     KIILNDMSDFDEGDLQLGNMMFGDDEQ"
     gene            complement(22564..22950)
                     /locus_tag="O0293_000303"
     CDS             complement(22564..22950)
                     /locus_tag="O0293_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279215.1"
                     /translation="MSKIQQRIRIEGKYLDLLTKIQRENDFKNSGEAIEYIINEYLML
                     KERVDETSELISNMKKITLIMNQNSKDIKTLVEFANTTAYLEGFDENRSAHSEPTSWL
                     ESAKKDVETQIEEKRIRKMTNKNRRG"
     gene            complement(22960..24108)
                     /locus_tag="O0293_000304"
     CDS             complement(22960..24108)
                     /locus_tag="O0293_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001631996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279216.1"
                     /translation="MPKIVTVSQFEQPNEFYEYSNYVNYMKRGEAQNNKDDFKYDVYA
                     HYMFDEEKSQNMFNEHDNFMSKKAINKIKSDFSHAQKNKGMMWKDVISFDNSALESVG
                     IYDSKTHTLDEQKIKEATRKMMKRFEKKEGLEDNLVWSGAIHYNTDNIHVHVAAVEKV
                     IKRTRGKRKPATLKHMKSTFANELFDIKGERQKINEFIRERIVKGIRDESEPYKDKAM
                     KKQIKKVHKLLEDIPRNQWNYNNNILKYIRPEIDKITDIYIKNYHSKDFEVFKNDLKR
                     QTNLYKETYGENSNYGSYEKTKMKDLYARSGNTILKNIKELDQDLMKVKQQSTHSKSN
                     ATITKLKIGKCLNQALYRTKYALRSDYDKEKNIQQYYQEFDKQSYRER"
     gene            complement(24129..24383)
                     /locus_tag="O0293_000305"
     CDS             complement(24129..24383)
                     /locus_tag="O0293_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCY0279217.1"
                     /translation="METRDIKIRSVDVETIKRIDRMAKQKNISRNEFLLDIIEQIDPL
                     ECYRSLYAEQSHQQAQNTKALKELADKIDRVYNTINDLEY"
     gene            complement(24637..25482)
                     /locus_tag="O0293_000306"
     CDS             complement(24637..25482)
                     /locus_tag="O0293_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279218.1"
                     /translation="MKELFRAFIPGKTSIRNKGSWLFYMLTVLIVGSFVLILGSRFFT
                     EDPVTNVHTPIKKWQNTSTSGSVRINQWTWNKEKKTMDFVIEYKQELGKDVIQNVQYK
                     IIPNATPDIESKYKIINQMDNKQAIRVYDVKPNFEVLVVKILSKDKESDNEEEILKVF
                     GNEKNVSIEQKKTLPTKEDYQLSFIKDDIEINKQNLKNKEKEKGELKKNIEDLNTEIN
                     LLDAKKTYQISDEKEETNNQIEAKKSEIKEKENEINNVEEAEKKLQEKIDLLNKKYQA
                     TNKIS"
     gene            complement(25495..26106)
                     /locus_tag="O0293_000307"
     CDS             complement(25495..26106)
                     /locus_tag="O0293_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279219.1"
                     /translation="MSKNKKHANKNGKLFGTVIILLSVIGLIIYFVFQDHNELNKTVE
                     IQSKKIKSLQKDNDEKQKLIERLDNKNLVKEEEELRGKAEEFVKVLYVTSTKTDDKKR
                     YEKAKELMDKSLADQYFGETRRSPIKYETDIQDVKIYNERYSPEKKEYHMYLTLTQAI
                     KGSSDGIDKRKIASELTLKQEGNNIWKVSKFKQFDEEKIEESN"
     gene            complement(26127..27233)
                     /locus_tag="O0293_000308"
     CDS             complement(26127..27233)
                     /locus_tag="O0293_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0279220.1"
                     /translation="MKKRTIAKRAAKQAVKFIAHKANIALWKIWLLLGAVLFSILLTI
                     VIVGTIITAVRVEKDKQEQATSSSKGCEVVGGNLKEGGLQTFEKNAKGGALEGKSEKI
                     VEIAKKNKIPPSLFMAIIASESGWGKGANATRQNNPLSVMGNKSIQDSTYKTIEDGLE
                     DGARNLYDLYISKGLTTPEKIGSKYAPVGAANDPSNMNARWVPTVKSNMKSLGEVEKG
                     GTTVSCDNDIPAGESIEKLKKVLEKHGGKLPKYGKKTFEPNTYAYHQCTWYVYNRRKE
                     LGLPVELSFGNGGDWADKAKAQGYKIGKTPKVGAMISWPYNSKAFGSTPYGHIAFVEE
                     VKKNGEIRVSEYNIIPFTYGERTFKLDGDETFIY"
     gene            complement(27236..29233)
                     /locus_tag="O0293_000309"
     CDS             complement(27236..29233)
                     /locus_tag="O0293_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279221.1"
                     /translation="MSSVYSRWRERRKVKKAIDSGTLSQSDFDIELLSQIQPRGGVNF
                     KNDFYVRKGTGYETCITIYETPTDVEDFWLGQIMTMDNTIITMDLRTKTKEEIKNNLK
                     TGMTEYLDRMHNDRDVVSRTEAEESYQELNTLLRNVRNGGESIKEVVIRLYVSGRTKD
                     EVDEKLNEIFVFLNAMDFKGTVYLNEQKFESQALTMSLTQQESVLPRIKKDIPSQTIA
                     IGVPFHSVSLNDEHGVYFGESDTGGNILLDLFYKDSRRTSYDGVFVGAKGAGKSHALK
                     KILLEEAIQGHRMRTIDSTGEFVKLVEVLGGKTISLDGTDGMINPLEVNAVAVREKVD
                     DLGKNVQEIDEVTSFNQHKHKLSTFYGLMNPDAKAKDKQLFRIVIDQFYKDIGLWSAN
                     PDKQTNITNLNPEDYPTFSELLQYLRELVYEDQEFKNVRPNISEDRLKQYQSIELTLQ
                     DIISSNGAMFDGHTTIENFKQFSIINFSIRQLNAMGDEVKKAQLFNVMNLLFDEMVYF
                     GSPQFQAYNRRELKLEDAVRYRLIFDEAHNLISASETDAPVVEKCEKFIREDRKYFAG
                     FIFASQDIKDFIPEDSQEQNVAKVKTLFSQTTYRFIMRQDTSNVEKLGNVFSQELSDS
                     ELSVIPQLGTGEVILNIAGVNNVKFKIDISEEENDLFGGGA"
     gene            complement(29253..30041)
                     /locus_tag="O0293_000310"
     CDS             complement(29253..30041)
                     /locus_tag="O0293_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279222.1"
                     /translation="MAMFKTPQETTKTSSNKTLFKEASSKKDDASSLFGKVFEEETIK
                     ISSKKDESTSTIFGDINETKIKKKKLRKKRVEHKNRPIKLFKPYRASKTFLDISNIIR
                     RTEDHKGGLYVLRDGTYMKFFQVNPKDIMSRNSHEQLYDMGVLLNMLKTYNKDNKIIA
                     LNMPIDTETQRSHLIRKYEKEEVGIYRYFLEQRIKKLEYLEQSSRMERSFLAMLFGKT
                     AQELNVNIQTYKKSMARGFPIKELTKEQEIKILYKLNNQCEEIK"
     gene            complement(29995..30360)
                     /locus_tag="O0293_000311"
     CDS             complement(29995..30360)
                     /locus_tag="O0293_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279223.1"
                     /translation="MSNRIPENTNVEAKITRWLYMKDVMTFLIVFVGSYILQAIRIVP
                     ESATLYMNILQLIVILTLIIRPFATNPKRRSLRVFWITVRKDRNVYAEIPYEMIEKDK
                     EVTNHGNVQNATRNYKDFL"
     gene            complement(30371..32212)
                     /locus_tag="O0293_000312"
     CDS             complement(30371..32212)
                     /locus_tag="O0293_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279224.1"
                     /translation="MRDSATQKVLEKYQDVLQMSEIVPNMLRWIGWYFLQFLVWITDL
                     VSNLTIQVLTLFGLLTQDSISRLILEAKPVLISVLALAIIYIGIKLMLGRPINKADIF
                     ATLVVAVLFLGSVNIGLQKGSELTKEASKAIENFDPDSKKQTDGTKVGSSMLAKSIVD
                     VQLMAEKGFPDVKTTRNNQKYNNLNASTIKHININQELEEKEVKKKESKEILKKKLVV
                     DEAGRQKVEDLDKNWFGLGDEKYYRFHVKWFTAAVGLICLALGHFLAALRVASIALEI
                     IVNKILAIFFSHALQGERLKSTIIDILNGFVSVIMIFFCLFLYKEVVSFLNDKPTGIW
                     LVGLIAATWFMIDGPKSIERKFGYDAGLRSPFYTMAGMYGMGKAATALATNTANGTSS
                     MIKAVRDDFSKSKRNDVNNNSNEQQSKSEQLNKASNNQNTDKQQNVKDNQNIDSNSQV
                     NDRQNNTESNSSHNSQSNSNRQSLDEEMKNSNYGNNTFKGVGESHSQLWANRAPDGGR
                     KKETLNDVIKRTQGEKSNTPKTNQLNTNTPKSNPMNAPKAQRKITDNGFSKQQNTSQN
                     NPSPKSYKDVQNVKPVQTNKPVNKSIPKSHVNKPINKNSSKNIKPKR"
     gene            complement(32218..32664)
                     /locus_tag="O0293_000313"
     CDS             complement(32218..32664)
                     /locus_tag="O0293_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279225.1"
                     /translation="MKNIVGFVIFLTVILLLSGCSKSGIEEISPKEAHDKLKNPKNEY
                     VIAISDLSRAGEEHIYNSKKILEKVAEEQGIKIYYTIYNVKNDKHQKDWNENGEFLGD
                     TSRLYFAKKGKKIDDMDYQDLAVKDTSNIELAISKLNKFIEGIEKR"
     gene            complement(32661..35435)
                     /locus_tag="O0293_000314"
     CDS             complement(32661..35435)
                     /locus_tag="O0293_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001210915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HCY0279226.1"
                     /translation="MRFGSKDKHGYRRKKLSFSQNKHLLAHPCILVSILLLIMLLATA
                     IANFLINILMTVFFGNHDIFTNMKQLNTKYTDYIFNFRLDAVLLYIPIWVLVFIFFGK
                     KVYQFRQRFKSLNNNEKASGRFATRKEITQQYKAIPQREHAFEGEGGLPVAMFGVYDF
                     VTKTKQYLEQRKTAIREINGITDLTADEKLELRKDKLGKMNKEFATTFFSKREFTFID
                     ESPTNNLIVGTSRSGKGEIVVLSMIENYSRSSEQPSLIVNDMKGELFSASYKTLENRG
                     YQVEIFNLDQPMESTMSFNPLQQVIDEYMKGDLGEAQEMTKQITYTLTNNEGVEDNEW
                     NLMAGALINAMILALCEETLPKNPEKVTLYSVSNMLQTLSTKRFYKEIAIGNKVQLIE
                     VSALDEYMERFPSHSPAKTQYATVQAAPDKMRSSIIGTALKALQPFTTDTIAKLTSHT
                     SFDINKLGFPSIIDGYATKDSTFELGVQVLRDGENGIEYEEVEGIQIGVNEYGYWQYP
                     FKTVLKVGDRIETIEKDGNNQVITSYFYVDHIDDKGQVTFNQKGELDNSDITIRKFNS
                     FPKPIAVFMVVPDYNNANHGIASILVNQIVFEISKNSQLYTENQSTYRRVIFHLDEAG
                     NMPPIPNLSQKVNVSLSRGLRFNFFVQAFSQFKDKYGDAYDAIMDACQNKVYIMATQE
                     QTLKDFSAMIGSQQITVHSRSGEAGAIKSSITENQEERPLLRPDELARLQEGETVVVR
                     NLKRQDKKRNKVMSYPIFNDGKYAMKYRYQYLSDLMDTSQSIIHFRPMLKARCEHRHL
                     QLEATLVDWDKRIEEMQEGIDGQKEQENKNINDINQYKQSNEEGNVAVRVKRLETLKE
                     KVGDAIFDKITRRFERYLRTYAEQGKNVNTITQKKLEELVSADNEVKEEEKTKRIEMI
                     QKFIKEAKTT"
     gene            complement(35450..35893)
                     /locus_tag="O0293_000315"
     CDS             complement(35450..35893)
                     /locus_tag="O0293_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000931137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCY0279227.1"
                     /translation="MLNEFKFIGRIARDLELTTKENMQARCNFTLAVNNAYGTDFLNI
                     TAFDKTAENLVKYNQKGDMVYVEGYVSTNKKSVNGVDKYYMNLVAGRIVYLSNKKVEE
                     SENELEHSTDNLDNLTFDDKQHTEVNENNTTFQKAKGFVTEDLPF"
     gene            complement(35908..37050)
                     /locus_tag="O0293_000316"
     CDS             complement(35908..37050)
                     /locus_tag="O0293_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279228.1"
                     /translation="MLRIKTKSFHIKNQSDFSLEEMMSYTEDMEAGKFTESLDKFDYV
                     MYDEQTYEDILESTYKVNSGYINIQQQIYTLLKATAPDTEELKRLKRQYLIALQEVDK
                     SYRDKKVAMNKKKNNPDNTKFQYQRQNNANKTKQKEILEKLKGKRGLMFLGLIFFVML
                     LVYILVVTDPNKKGASDVATKEEIYQQALLGEEEKAIDNFAKLDQETLTKKDKQIYAQ
                     LLAQKEEYDKAVKLSDAKFVEAYLISRDYYSALEEFNKKYPTNNGKFDLAIHQNKFSD
                     AINYSQGIDKTNNRKKQLVKAYIETDNLNKAKELAATTEDKEVTKMIADEQNKRLKGL
                     EEAVKSKEDDLKKAKDKKEKKSDIENKEKALKEANENLEKFKKELK"
BASE COUNT        11518 a         5903 c         5251 g        14396 t
ORIGIN      
        1 aaaaaaaacg aacaaattat taatttgtcc gtttacataa aaattatttg taatgtatct
       61 ataactgcaa acttagtttc tgtcgctgac ggaaatactg cactgacatt aactattttt
      121 ttaaatgaac gtgcataaac aagggctcca ccagataata ccgccccaat tattgcgcct
      181 atcaacgttc tctttgccca gcgtacacca gtatccccaa acatcattac agcaccggaa
      241 acaaatatcg aaagtgccaa taatgtaatg ataagtggtt gagcatcgtt aataagggct
      301 acaatttttt cattaagtcc gttaaacatg ttttattcct cccaagctgt attattttta
      361 ttttcagaat aaaaacgcaa ggcttccata acaacatctg tattttctga taaataattt
      421 tgatattggt tatacagcca ttcgttatgt gtttctccag ttactttcat tatttctgtc
      481 acttgctttt ttaattgatt taaatcattt tgtgtaattt tagacatgat aatttaccct
      541 ttcttttatt ctaaaaagaa aattaggtga aacaccttat tcttttttaa attttgcttt
      601 atactaaaaa aggacttttc tttaaagtcc tcacaaaaat attattctag ttctttgata
      661 attacatata tgtgcctata ggctatttaa tgtagctcta gcatactttt aactaatctg
      721 tcttttcaaa cagtcaataa attttacggt aagtaaattt cactcttatt aaaataagca
      781 aacactgctt aaacactttt gcttatactg atgtttttga cgctttttag ttcttgactt
      841 actttcttac gataagcaat tagtccataa ttgtcattaa ataccgtttt cgttttataa
      901 ataacttctg acaaatggcg tagtgttttt ctctcgttta tatccaaacg tttatgatca
      961 attctctgac aacgcatacc tttgattcga taagttttac catcaaacgt acgtatttca
     1021 tcagcaaaaa agtaaaaggc tataaaatca aaacgtttat ttttcttata acaaagcata
     1081 aaacaacacc tccatgagta aaagttatta cgattatcta gtatctcttg atgttatgat
     1141 tgaaatatat aaattataaa atatgggttc ggattgtcag cacttttcac tgcccgaccc
     1201 ttgcggttct ttcgtcttcc cgctcgcgct attcaggctg tatttgtttc atctggttca
     1261 tcgggacttc ccctcccagt ttatacacaa aacagtgttt gattagaata gtttaataag
     1321 aatgaaattc acaggtttag gattacccgt ttaaccaata gtcgtatacc ttatctgtat
     1381 tgaattcctt ataatacagg ggcacgcata tgtaattatc aaggttcaag taaccattaa
     1441 tggttactat aattatagca ttacttaata ttttaagcaa ggagttatga taaaaagtgc
     1501 aaaaatatcc acgtgttttt agaatcaaat ctactacaaa acagttaata aaaataacaa
     1561 gtgcaataaa cgatttgaat aattcagatt ttgtagaaaa aatcattcaa gaaactgaat
     1621 taagcaatat tgattttagc agttacaata attacggtaa aaaaagctat aatactgtac
     1681 aagcaactta tacaattagt tattcaagta atgaaaaaat tgaagcagta gcacagaaac
     1741 acggattaac acgatctcaa gtgcttaatt tgtttttatc aaaatatttg aatattccaa
     1801 tagaccataa agactaaatg tgtaacactc tcaaatgttg cacattttta ttttactata
     1861 tttcttttta atccgaaata atttttaata tatttacacc ctagattgtt ttaatatttt
     1921 ttgtcagaca aagaagaggc tattgccata ataaagaaaa tcataaacac aaatttgatt
     1981 gtggttaaag taatgataaa gaatattttt acaaccggaa taaaataacc tctaaaaaac
     2041 atcatgtata tactccatat agcaaacaca ggaataaaaa cataccccca aaaaaagcct
     2101 gttaaattaa gcaaggcaga taaaacgtat aaaaaaatga gcactaaaat gacaaaacct
     2161 tttaaatttt cttttgttag gccgcctata tagtttttat ttcgcatagt tttctctcct
     2221 ttcattttta caaaaaagta tgtttgtaat attaatcatt ttggctaaaa atattccaag
     2281 aactattttt aaaattcttt tttcgcatgt ttaatgaatg taattcttca agttccattt
     2341 ttatacattt catcaaatat ccttgcatag tttcaacact ttcatttttc atataaactt
     2401 ttgctttaaa gcgttttaaa acatttacaa ggtcataatc gatatcttct agcatataag
     2461 ttgtgttatg ttcagtatta aacgatttct ttgcttttaa aataatttct tttactactt
     2521 tgagttctct ataagaaaaa ttattaagat agtgttttga ttgttcaggg aattcttgaa
     2581 gttctgtgaa tttcaaagct tcttcatcag aatttagtga atcatgattt gaatgatttg
     2641 aatgattact aattttattt ttgaactcta tatcattcaa gtcattcata tcattattgt
     2701 cagtatcaat atattcagta ttacttaaat cagtatcatt agtgtgcgat tttcgcattt
     2761 cttgttgtgc gatttcagca tttctagaaa tgcgattttc gcatttcaag aaatgcgaaa
     2821 atcgcatttc agcgtcatta ctaggttcaa cacttgtttc ttcttgtttt tttaattcat
     2881 aaacatcttc tttagtaatt gtaggattct tcaaatataa tttgtttggt ttaccttgac
     2941 cacatctgac ttgttctaaa agatcagctt tagataattc ttttttaatt tttgttaatt
     3001 ttccatcgtg acaatttaat atagttttaa gattttcgtt tgtgaagatg aaaaatatat
     3061 caccattttc atcaaaccaa ttatttttaa ttgataattc tagtctatcc tttaacaatg
     3121 cataagcaat ttttgcatca ttgcttaatt gcatatattt ttcgttagta aaaaatactt
     3181 ttggtagttg gtaaaatttc tctctatatt gttgttggat attaaatctt tgttcagaca
     3241 taataaaaac ctctttccga attttagttt ggtaagaggt aataaaacgt ttacattttt
     3301 tgtgtttatt taataatcta ggtgcttgca ctaaagagaa aatcattata taatacaagt
     3361 atcaatttta tattaaataa acgtttgcag acgttctatt accagagagc aactactgga
     3421 attagttgct cttttttcat ttcttataga ttaataataa catgttgtta ttatttgtaa
     3481 aatttatttt tgaatccact tacattatta cttcgctatc tcttaattac aatttaatct
     3541 tttccacttg caaaaagcat tgccgtaaat actatgaaga tcaaagctac tatagttcct
     3601 aatagaataa agattgcaga aatgatattc catacgataa aaaataattt tttacccccc
     3661 accacttcaa tttatttatt gcaaatatta aaagacatag aatgcccaca aatacgagtg
     3721 atgaaaaaga gatatttaca gtttgaatat ccatagttat cacctcttta ttagattata
     3781 ccgtaattta aaccattaaa caagtatact agtataatag tttaatggtt taatgttaat
     3841 cttgtttaac agtatacttt gtgctaaact tagagtatca acaaaaaaga ggtgattaat
     3901 atggattttc catcaaagtt ttggagtaac aataaaactg aaaatgaatt gttaaataaa
     3961 attaaaaaca aagatgaggc tattgttttg acaataaata tgtttaaggg tggcgttgga
     4021 aaatcgactt taactaatat gtttggttat attgccaata gatatgattt aagtgtttta
     4081 tttatagata ctgatccaca gagaacttta acaaaaaaat tgagtaaaaa ttttagtacc
     4141 aaaaaagaag caaatttaag ttttatggac ggtataaaaa caggtacact tgaagattct
     4201 atttcaactt taagcaataa tatagatctt attaaaggtg attggaattt agcttggttt
     4261 gatagatatt caagaagatc attaaatgtt aaagatgaat tttacgttta tagtagtcta
     4321 ataggaaaac taaagaaaaa ttacgatttt atatttttcg attctgttcc aactacaaca
     4381 acattaactc ataattgtat tgttgcaagt gattatgtga ttgtacctgt tgaatcagaa
     4441 gaagattgtt ataataattc aattgattat atgttctacc tttcacaaat gaaacaatat
     4501 aatagtaaat tagatgtatt aggtatactt atctatttaa cagaagaaga taataagaca
     4561 aataatgaat atgtaaaaaa gtatcgtaat gaatataatg atatagtgtt aaaaaatgtt
     4621 attactagat caaggagagt tatgacatgg ggatcacaag gtatttcaga acacaaacct
     4681 tatgataaaa aaacattgaa aaaatattta aatgtctttt gggaatttat gtatagacta
     4741 gatgaaagag gtgcttttaa tgagtagtaa tttagataaa tatagtgcaa agaaaagaga
     4801 aaccaatgaa attaaagaaa caccgtttga taagaatata aagaagaaag aagattttaa
     4861 attagttaag attgatccaa aaattcatgt agatttaaag tttgcagctc taaaagaaga
     4921 tacaacaatg aaagcggtgg ttgataaggc aattaaagaa tacttggaaa aatatcacta
     4981 acaagtaaac taataaatta gtttacttgt atataagtat acttttaaac tagtatactt
     5041 ataaaacgaa aattaaatcg taggtattct tggcagaccc tacgactaaa gcatttataa
     5101 aaatacttac acgagttaat aaagaatata taaaagaaac acatatttat aattatcaat
     5161 ttgttttata gcgtctatat aataggcgct ttttttatac aaaaattttc gaaataatag
     5221 taggcaaagt agacaaagta gacaaaagat gttataatgt aattaacaaa taaaagaaag
     5281 agggttttat aaatggaaaa caaaacacat tattttgaag cacatggaaa agattacaag
     5341 ttagaagttg cgaaagatat gttcggctgc gagggtgtta cagtagttga aaatggtttg
     5401 tatatgggaa tgatagactg tacagacgag agagactata agcgaattga aagtatgatt
     5461 agagcagata agcattttgt ttatactgat gaagtttatt gttaaaaagg ataagttaaa
     5521 taaataactt accctcagca taaaaaaaga aagttgacgg aacaattgcc gttgttccgt
     5581 ccatattata acaaaatatg gagggtttta aaatgtatgt agcaaaagaa gaaaacggac
     5641 aaattttatc ggaaagccca tttattagtt gtgtaattca agatgttatt gagcagagaa
     5701 tagaaaactt tgaaagatat tacaatcact ataaagagaa tttggaactt aactactata
     5761 tttatatcgg aaatttaaat aaactttata acagtattga agattatact aacgaattgt
     5821 ctttaataga tgatttttat aaagcttata atgatgaaag ttttgttcat aaatctcaaa
     5881 tagtgaggat agaaacataa atcgaggggg attggtttcc tcctcataat taccctataa
     5941 ttaaagtaaa cataacaatg gaggtaacct agaaatgaat gacaataaaa agaaatatga
     6001 aagccaaact aatagacgta atgttaaggt atataaaaaa accgacgaaa ttgcaagcga
     6061 attaaaattt gaattacgtg ttaaaaccaa acaggaagtt tacgatatcg caatcgaaaa
     6121 actgtatgaa caaataacag gtaaaaaata tgaataagac agtaaaatta ataacttttt
     6181 gaggaggata ttaaaatgaa caaaaaatta atagcaagcg gaacaattgc gtctatttta
     6241 ttgttaaaca ctggtggtgt agcatttgca gatagtaaca agcttaatga agatatcttc
     6301 attaaaggtg cagacttgaa cagtcaacaa ttggaagaaa ctaaaaagaa gcttgatgtt
     6361 gatgatggct ataaagaaat ttctataaca actaatgatg ttgctaaata tactggaaat
     6421 aacaatttaa gctttattca ttctagtgca actatcacac caaaaaagtt taaaaaaggc
     6481 gtaacagttg atatagaaac gccagaaaac attactagaa ttacaaaaga acagtataca
     6541 aatgcggcta tcacagcagg tatacaagat tctgaaatca aagttgcttc gatcgataag
     6601 gtatcaggcg agggtgcttt aacagggatt tacaaagcat atgaagaaaa tggcaatagt
     6661 ttaaacaaag acgatgttca aaatgctaat aatgaaatga atgacctagc ttcaattagt
     6721 gaacagaata aagggcagaa gggttattca gatgaggcgt taaataacgc tattgctgaa
     6781 atgaagaaag aaatagcgag tgaaaaacaa gatggaaaga atttaactcg tgaagatatt
     6841 agtaagattg ttaatgatat tcttaaagag aatggcttga ataagatttt aagtgataat
     6901 caaattaatg tgattaataa tatcatggtt aacgtttcta attcagaaat actaaacaaa
     6961 gaccctaagg cgtatgaaaa acaagctaag gatttagcaa gcaatattaa agacaaagca
     7021 ggtaatttgc tagataaggc taaagaacta aatacagaag aaaaccgtaa tttcttacag
     7081 aaaatttggg actctattgt tgagttcttt aaaaaaatca ttaattggct tttatcatgg
     7141 ttttagtggc taaagaataa taagaaaaaa agccgtagac ttctggggga agctacgact
     7201 caagcattta taaaaacact acaaagataa tcataattta taaatgctga ataatcaaga
     7261 ggaggcattt attatgaata aagaaacatt gattgacctt atagatatga tgataggatt
     7321 aactgaaatt gaaagaaaaa gattgtcaga aatggaaatg cgaaaagtag aaatacgtta
     7381 caaaatggca ttaacagaaa aaacagatga aatgatagga taatacctaa taacgcttta
     7441 gaaaggtact atttcatgaa aaaaatatat atagaaaata ttgatgatta tatacttgtc
     7501 gatgatgaag attttgaacg tgtaaacttt tacaaatggc acaaatcata tgctcacgac
     7561 acacaacgtt ttttagccca aatacttggt aaaaaaacgg cactaccagt attcatactt
     7621 aacaatcaat atgcttatca aaaggttaag aataacgatt ttacaagagg caatttaggt
     7681 gctgataaac attctttcag atatcgagca cctcaaaaga atagtagttc aaaatacaaa
     7741 ggtgtttctt atgatagcaa acataacaaa tgggttgcta gtatctatgc agagggagag
     7801 aaaaaatatc taggtagatt tgtagatgaa aaagaatgtg ctaaagctta taacaaggct
     7861 gtgcagaaat attggggcgg agacggttat ttaaacgacg tataaaaagg agctgttaac
     7921 aaatgtgcga tagttataaa aagttaccgg aaaacgtcat aaaattcgac gctataataa
     7981 agtcgaaaaa tgacaaggtg gaatgtcagt gcggagacaa ggctaaatat cgaataaacg
     8041 aaagatataa gctagtaaca tgcgaaatct gtggaaagaa tgtagaccca tttacagcat
     8101 tattagtgat tgtgaaaaga ctaaagaatt tttatcataa tagcagaaac tgatagagag
     8161 cgtgcattat taggtaatgt tattgcaata ttcaaaacat attctgtttt acttataata
     8221 gcaccaataa cacaaatttt aatttataat cttgccttag attttaaatt tcaaaaatca
     8281 agttcaccaa gttcactaaa taaaaaccaa tgataaaaag tcattggttt ttattattcc
     8341 acactaacaa tatagtttaa ggatattttt attttagttc ctctatgttt actagtaact
     8401 accattacac gttctaacaa gtcaactttg tttatatagc attccattga atttatatag
     8461 ccgttatccc aataacttat ttctgtaaga atattgttca tactgttgta gtaaactgtt
     8521 tcgttaagta tctgtaattg atcgtcagac aacaaaggca tatcaacttt gttttgttct
     8581 tctatatact catctaataa cttgtactgg tcagagactg ttttaaatgg agaccatttg
     8641 actttatctc ttcccttagg aatattgcta ttaagcaatt ctttggggat ttttctataa
     8701 tcagtttcgt atttatactg catgggaagt tctggattaa tcacatacat ttttaacacc
     8761 tcagctaaag tatagaacgt atgttctgtt ttgtaaagta tacttaagaa aggatttgat
     8821 atatatgaaa aacactaaaa attggtatga gtctctattt tcaggggttt atgatggctt
     8881 gaaaataagt ttccccctag tattgattta ccttatagca tacattcatg attctctttc
     8941 tttcaatcca agcataaatg ttattagatg gtcatttgga gttatcgtat caactttgat
     9001 attcttttta atttttaaat tcaaattttt cgctaaagat tggaaactaa catttactaa
     9061 caaatcattt ctcatagtta taggctcatc aatacttatt aatttaatag gttactttca
     9121 aaatgtattt gatttatata aagaatcgaa aaatcaaaag attatagaag aaaaacttca
     9181 aaacaatcaa gatcatcttc atttctattt tgttgatatg gttattgtag caccaatata
     9241 tgaagaaatt atatttcgag gaattatata tttttacttt ataaaagcaa tcgagaagtt
     9301 attttctcag aaatcgtggg aaataaatgc tagatatagt gtcattatgt gcaatggaat
     9361 gtttataatg acatctagtt tgttatttgc atatttgcac gcatgggata catatataga
     9421 agcaatccct tatttaagca tgggtattat attaagcgct gtagttgtta tatcgaaaaa
     9481 tataatcaac tctatattga tacatatgat aaataatttc tttgctttta tgtatgtaga
     9541 aacaaaagaa atattttcct tgttgattgc gttaattgca gtagctatta ttatgtgttt
     9601 actggagttg aaaagaaaaa gaattagtta agtgcttaat cttatttctg acagcaaaaa
     9661 acattagtag gacgtaataa aagacgagta tatctcgtct tttattagtt atttattaac
     9721 ttattaataa acagataata gttgttactg caagtataca ttgagctgta aatatcgctt
     9781 tatcggtatt gcttgcttta atttttacat tagctatatt taggaaattt ttgtgtatac
     9841 gttttaatgt cttgtatttt attataagca taatatttat gataatacta attactaaaa
     9901 cgattgttct ttctcttgtt gtagggcttg tattaataat taaattgaat attgggaagt
     9961 atatttgaat tatagctatt ataattatag gtacatatat atatctgaaa accttagcgt
    10021 gcttttgtaa ggctgtttta aactgttcat tataagatat atattcagag ctacattgta
    10081 cgctgtaatt tgcttggtta ggaaaaacag aaaacatatc gtcccagaat gtgttctgtt
    10141 taaatagttt tatttttagt atctcctgca catcttcgtt cataccagca tttgataaaa
    10201 cagcagataa ctgtagaatt tctttatcag agagcgatac tttctcatca ctcattttaa
    10261 gtagtttgta attgttaata actgtttcaa agtttttctt atataagacg tgtcttaagt
    10321 tattaggtat attgtctttc tttaagttaa tatagacaaa taacattttg atagttttaa
    10381 gtacaactaa aaacaacgat aaaataaaga atgatatcaa tattgatgat gttaaaaagg
    10441 tagcaataat aaaactatcg tctcctaact tttcaagtga aatatgcgag ttaattaaag
    10501 cgctatagaa tttagataaa agccatattc ctattattag atataaagag ttgagaattc
    10561 ctaactttaa tactctgttc aatggaatat atctagtatc atgtttaaag aaattaggat
    10621 tgaaacaatt taccgtaata taaactattg aaaaaattgt taaaaaagta aaaaccaaag
    10681 agttaaaagc atctatatta ttcatactta tccccctttc tgtcattact aaaaatagaa
    10741 atgcttataa aatttaattc gtaattatac ccaccgttat ttccgttcac atatattcca
    10801 gtttgagatt ctccaagttc atttaaatta gaagttatta atacgactct atttccaaga
    10861 tgaatatgtc tttttaaatc gatttctaga tcattataag aaaaatcaat ttgaaaatct
    10921 gttatgttat caagaggcgt tttatcttcc acaaaatcac ttgaaatttt taaactataa
    10981 gcaaatgtat tccgtttaat ttgaatcgga attagctcgt ctaattttat tttagaaatg
    11041 agttttgtac tttttttatc ttttgacatt gtgaagcttt tttctaacgt tatctcacta
    11101 atatgtagtg actttgttat atcgtttata ttatcaactg cttcttttaa ttttgttata
    11161 aaaatttgaa catcttttgg aagttttacg tttaaaatgt ctttttcaat cgttttagct
    11221 agttctaagt aatcttttct atctaaataa tagttataca atttgtttac agtttcaaaa
    11281 gtcgctttag aaatgtccgc agttttcctt cttaaattag agatagtcac tttgcttatt
    11341 ccagtgtcat tagcaatttg atttgtttga atatcgcttt gtaatagaat attaatctta
    11401 cttatcatat cgttgttttg cattataagc acctcctgta atacatatta taacactaat
    11461 attaactaaa tttaacccat tattttattt ttggttaatt gtaattaact tatttgttga
    11521 tagttccttt attatagttt ataatatata aaaagggaac tttaaggagt gagtctaatg
    11581 tatcaaacga atttaaatga agttataaaa aatgtcgaaa cgttattaag aagttctatt
    11641 acattaaaag aaataagcga atccacaggc atatcagaaa gtgtgttaaa aaaattaagc
    11701 tcaggcgatc gagaagtgtc aaatgcgaaa tttgaagtta taaatcagtt gtatcaattt
    11761 tatctggaaa atcagaataa aatatttaaa gataggtttt atatggaaga actatctaga
    11821 gtcaatttac ctaaaaatat aagaaatttc ataaaagatt taagcaatgc aatagaccaa
    11881 gtaaataata atgaacaaga aatgttatat gaggttagaa ctatttatat taaagataaa
    11941 aaggggaata taaaagaaaa aggaaaatgt attgctattg atgaatatat gggcttagat
    12001 ttagatgtta ataccggtca tgcaaaagaa atatatgatt tgaaaattag taatgaaata
    12061 gcagaccctg tagatgaatt gaagcaagtt aaaataattt ttgatgaaat agggcttgta
    12121 aaagcattaa agcaaattaa gtatgacggt ggtaagatta aactttcaaa agaaaagcgt
    12181 catataatgg tttacccaaa agggacgtct ttatatgaat ataatagatt tgattatata
    12241 ggttcgttcg agcgtatgtt cttttcttta gagtataatc aagaaaagaa ttatagaatt
    12301 tttaatgaag atgtctcctt taatgtgttt atacaacaaa taatagacta tttaggaaat
    12361 tatcctgcag atcccaaagg cgaacgttta gcggttgtaa gacaaattat tatcgaaaat
    12421 ggttataaag acacggaaga tttcttaaac cgatatattt tcggagaaca gtcctaaaaa
    12481 tgaacgtttt tgagacaaat cccctaatga taaattaaga agatagatta agaaatttat
    12541 ccttgaattt aatggggatt ttattttggc cataattcct ttaacttgtg ttataatcta
    12601 tgtatcataa atttcaaaat atacgagttt ttagacgatg acagacttgt ttatttgcat
    12661 cgagagttaa ccttagtgtg taagtactaa taaaacgatg aagagaggta atatttaaat
    12721 ggctaaaatt ggttatgcac gtgtatcaac gaaagatcaa agtcttgacg gccaaattga
    12781 tacacttaaa gaatttgggt gtgaacgtat ctttagtgaa aaaataagtg gccggaaagt
    12841 caaacgtaca gagcttgata agtgtttaga ttatttacga gagggagaca cattagttat
    12901 ctataagctt gaccggttag gtagaacgac aaagcaattg attgaattat cacaatggct
    12961 cgatgataac gggattgatt tacaaattat tgatatgaac gtttcaacga aagacgcaat
    13021 gggtaaaatg tttttcacga tgatgagtgc ctttgcagaa cttgaagcta acttattaag
    13081 agagcgtaca aaaaaagggt tagctgctgc gagggcaaga ggtagattag gaggtagacc
    13141 gtctttacca gaccataaga aacgagaaat taagttctta tacgatgaac aaatgacgac
    13201 tggagaagag attgctaaga aaacaggagt aagtcgctct actgtttatc gtatagttaa
    13261 ggaaagaaag tgatataatc actttctttt ttatttgtaa aattttagaa tttatttttt
    13321 gtgttgatac gctcacacgc acaatcagag attgtgcttt taacaagcct acgtgatatt
    13381 gcaaggggca aaaaactttt tatttgtggc catatggcca gtctaaaaac ttttattgct
    13441 tgtacccttg caatctccag cgtaacggct tgtttcagtg gctatacaac acgaaaaaaa
    13501 taaataattt ctaaaagaaa aaaggagtta aaaaatggat aaaaacaaac ttgaaattta
    13561 tttgagaata tgcgaaaaca tcaaggaata taaaaattta gaatttgaat gttataacga
    13621 agatgaagaa gtttttgatt caaacttaca atgtcctttg gcatatacta caaaaggaga
    13681 taatgaggaa tttgaaattc aggttacatt agatttaaat aataaccaaa ttattaaaga
    13741 aatatcgcat gtgtatataa attataaaga atatgagtgt tttaaagatt gggaagaaat
    13801 agcatctaaa actttaaact ttgacgattt aattatgact gatatggacg tagatgattt
    13861 actagaagaa attcctaata aaaaaggaat aaaatattaa ataaacaata agaacaaagc
    13921 aaaaatcaaa atgagtgaat gtgatgtatt agctatgcac atcacattca catttaaaaa
    13981 ccaaattcaa aaatggaggg aaagtgaggt atcactttcc ctttatttca ttttttagaa
    14041 tttatttttt gtgttgacac gctcacacgc acaatcaaag attgtgcttt taacaagcct
    14101 acgtgatatt gcaaggggca aaaaactttt tatttgtggc catacggcca gtctaaaaac
    14161 ttttgttgct tgtacccttg caacctcaaa cgtaacggct tgtttcagtg gctacacaac
    14221 acgaaaaaaa taaataattt ctaaaagaaa aatcaaggag gatttaagat gacttactta
    14281 ctaatactca taataattat tggtttttgt gtcatgtgtt ttcaaatatt acagtgtatc
    14341 aagtctttca aaaaataata taaattttga aagggggtga tattagtgaa aagaatgcag
    14401 agaaaggaag aagaacaaaa aaataagact gtaaaagcta atttggtatt actgtacctt
    14461 aacatagctt atacaatctt acagatactt ggcaaagtat tatagttctt caaatagggg
    14521 ttcacgtccc ctatttcttt tctactatta taacatagga ggtttaaaat ggaaaattac
    14581 aataaacttg tgtttatttt attactattc cttacgtgtt tgaatctatt aaattttata
    14641 ttgtaggttt tataatgatt aaatgcaaac agtttcaaag attaaaagag cattatttta
    14701 atggtttaac tgttcaatta atataataaa gcgctcttcg gtaaattaca gatgtgtaac
    14761 tatccaatcg cttaaatgtt ttttgtgtac ttacaattag gatagttaga acagcctaaa
    14821 aactggccgt ttttgcctgt tctaactact aatttatgat tgcatcttgg acatttttca
    14881 ttatttgaaa aatcgggatt tatactaata atccatttag ataagcttgt tctatctttc
    14941 ataacgacat cgcacgcctt acctaactcg actgcacttt tagtataaaa actattagtc
    15001 acaagccatg cttcatcggc attgtaaaaa cgttgtgcgg tgtatatttc ttgcacagct
    15061 ttaataccta tcttactgtt actccgtttt gcttgtacaa ctattttttt attctctttt
    15121 tttaatatta agtctgcacc atagtccctt gaagctttcg ttacaattgg cttatatttt
    15181 aactttaaaa acaatgcttc gagataatat tcgaattcta tgccttccat tttatctata
    15241 tcaattatat gtgctcttct atataatgct tggatttttt tcttcttaat aaatctataa
    15301 atgacgggcg ttaacttata tgcgataact aacataaata ttagtagtaa aatattgtag
    15361 atgtttattt cagtcatgtt tatatctccc tatcatcttt ttcgttatga gtttgttctt
    15421 tcaattgctc ttttatttca tttttttgat ataacgtttt tccgtcagtt atcgctttat
    15481 ccttttttaa ttgttcgttc caatctttag agtttaaagg taaatctatt gtaaagtcat
    15541 tttcgtaagt attaacgatg ctgcttacga aatttttccc tgcctcatcg ttatcaacgg
    15601 ctaaaactat actttctagt gggtattctt ttggttcttc tcctagtttc aaattatatt
    15661 tgccaacatc taaaatgtgc tgtttaaaag tttctaattt aaggcctgac agtgaggcta
    15721 ttcgagtttg ttttaagtcc ctatgcgagt cataataact gagggcatct ataggacttt
    15781 ccgttataac aacattacga atacctatat ttttatcttg aataatatca atgtgaaatc
    15841 cgctatgttg ctttgatcct tctacaattt gtttaaaagc ctttctccct gtaccacgtt
    15901 tatctacccc gacaatgtta ttttcattat ctaaccattt aaaacaaaca ttgttctgtt
    15961 tgtcttgagc tataactcct gtattgaacc cccatctaac aagttttggg tctaaacacc
    16021 tttcatttac taagtaatta taagctcttg tactatcttt catttcagtt tgtgtattgt
    16081 aatttaaagt ctttttttct tttatagaaa aattgtcatt tttatattga gaaaaaccat
    16141 cttgcgttaa aatagacatt gcttctttaa aatccatgtc taatacatct atacaaaaag
    16201 ataaaccgcc atgtcctttt acgcctttag agttccaata ataactttgt ccttttagaa
    16261 tcaatgaatc gtgctctatg catctatcat atttgccttc ttttttaaat gtaagtccat
    16321 aagcttctcc tatttctcta atgtcagttt ttcttgcatg ttctttttct tcgtttgtat
    16381 aggattgttt actcatatta aaaacctcca aaaaagaaca gtccttgatt aaggactgtt
    16441 ctaaatattt ttatctttca taattttctt gttcggcaag ttctatatat tcttttggaa
    16501 gaggattttg attatttgat ataacgtttt ggttggattt cacaacagag ttttctttgt
    16561 tttcagaata tttaaattct cctgtttggg tattatacgt aaattcaaaa atatctgcat
    16621 tcttttgtat tttctcctta tctttatcgt ttaaagttaa cgcgtataaa ttttcatcaa
    16681 cttctacaat tatttctcct tgctctattc caacaaaatt tgccttttct aactcagatg
    16741 gttctttttc tggagttaca gcaaaagtat tttgattgtt taatgtttga aataactctg
    16801 attcctgtat ttttcctgct tcgtatatct ctttttcccg ctctaattct ataactttat
    16861 tttcttttat agcttgttcg gattctttag taagtgagcg atgaacgcct tttaactcct
    16921 tgttaaaatt tttcaagtta ttctctgtaa aatttaaaga tgtatttctg ttattttcta
    16981 aatgattaag tctactttca tttaagtctt ttgcgtttat acttgcttct attttttgta
    17041 tgccttgtag agtgaattgc tctaattgta atttattata atcgttatct atagagtaag
    17101 gatcactgaa atgaattgcc tcattagcat ctttaagata attatatagt tccaacattt
    17161 gcgtttggtt atcttgatta ctccaatcta tgtgtttact gaaattattg aagttatatt
    17221 tgttttctgt ttcaggattg tctttgagcg ctagtgtttg ttctataatt tctaatcgag
    17281 ttatttgttt ataattcctt aactctttaa tattttccgc attgtttatt atgttataac
    17341 tatccttcat catatcttgt aagtcactaa acttttcact taacaatatt tcgttattta
    17401 gctctgtatg ccttaaattt tgttgttcct ctttaaaaag tgtttttatt tttgaaaatg
    17461 catctaccac gttatcttta cttttgccta acaactcttt aatattttcg ctgataattt
    17521 taggatttat ggcataatcg gagagaaaag ccttgattcc atgtatattt ttattgatgg
    17581 tttctcctgt gaatttttca tgtaagcgcg ctttaatgcc tgtaatagat tgttgtaatt
    17641 cagtgaaata accacttagt cctaagtttt cacgcgattt tgaactcata cgttttaaaa
    17701 gttctttatc aacttgtttt gcaattagct cgtgcaaagc ttctttgtct atatctattt
    17761 tatatttttc cttcaaaatg tctttgatag gtgtttccat attattcgcc tcaattctag
    17821 atattattta tattgatcgt attaatcaaa actaatgtta tatctcttcg ttcctgtttt
    17881 cttatcttct atcaaaatta aatatgcttc atacgttttt ccagctttgt tttttaattt
    17941 caattttttt gaggttttac cattaaaaat caattcttga atttgtttgt ctgttaattt
    18001 tttctgataa aaatttttga agaatacttg ttcacaattt gtgcaactaa taattttgtt
    18061 tttatctatg agttgaccat ctgtacagtt agggcattct ccaaattcat tatcttttgc
    18121 aacaatatta ctttggttga ttatcgactt gtcttcacat ttctcttggt atgctttaat
    18181 ttctttatct atgaatttca ttgttatatc aataaagtta tcagtatttg cttgtccttg
    18241 tccgatgagc tttaagcgtt tttcccacgc tgctgtcatt gtagcactag caagcagact
    18301 cccttttact gctttacaaa gcaaaatccc ttttgctgta atgtgcactt tatttttttc
    18361 gattttgata taatttttat tttttagact ctgtataatt ccatctctgg ttgcttctgt
    18421 gccaatacct tgtatttctt ttaatatttc agcttcttca tcgtctaaat acttcccgca
    18481 acttttcatc agtgttataa gctgaccttc tgtataaagc tttggagatt gagtaatccc
    18541 cttttttgaa taaatattag cctttacaca ttcatttttt atgagttttg gtaattgttg
    18601 ttctggagat ttttcattgc ctttgtgttt aaataggatt ttccagcctt tttgtatttc
    18661 ggtgtgacca gttgtgtaaa aaataagttg gtttacattg gtttcaattt ttgtttcgtc
    18721 atatacatag tcattagcaa acatacttag tgtagtactt actacttcat caagcacata
    18781 tttttcatct tgacttaatt tctcaatttt ttgtggtgta ggtatttgag aggtaggtat
    18841 aattgcatag tgttctttta ctttttcgtt atttacatat cgagctcttg gcttatcata
    18901 cacaattgaa aaatgtttgt tatatacatt cattagtgat tgcaagtttt ccttcaaata
    18961 aagatattct tcactcgtga tgtagttaca atccgttctt ggataagaaa gtattttttt
    19021 ctcatacaaa ctttgaacaa tattcttagt tttttcagga ctatatttat actgtttatt
    19081 gattttagat tgtaatgttg ataaagagtg tagtttaggt gatgatgtgc gcttttcttg
    19141 agtctctagt ttagtaatta tgccttcttt agcatctttc aaattgtgct tttctcccaa
    19201 attaataatt tcttgtttat cagttgtttt tattttggct tttcctttat agcaaccatt
    19261 tttagattcg aactccccaa atatttcata aaatgtttca ggtttaaagt tttcgattgc
    19321 taagtaacgt tcgtaaatca tatataacgt tggcgtttga acacgcccga ttgagaaaac
    19381 atcttgaaca ccttgcaatt gaaatatttt tgaatataca gggctcaaat tcataccaat
    19441 taaataatcg cttgtttgtc ttgcgtaagc ttcttgataa ttttgataat cttgatggtt
    19501 attttgtaat tgttgaaacc ctttttttat ttccttttct acaagagatt ttatccatag
    19561 tctttttatg gttttgtttt tgactccggt cagttttaat gttgaataaa agatattact
    19621 tccttcccta tcaatatctg ttgcattgat caaaatgtca gcgtctttaa ataaagattt
    19681 aacaatgtta aattgcttgc ttttaccttc tgatactttg tattcaaatg gacttggacg
    19741 ataaggtagg ttttttaaat cccaattatt tatagggttt gagtaatctt ttggcaattt
    19801 taattccact agatggccaa agccccatgt gattatgact cccccgtcaa acataggatt
    19861 tacaccttcg atataaccat cttttttgtg tgatatccct aaagcattgg cataatctct
    19921 tgcttgtgag ggtttttcag caaaaataac tgttttcata ttgatgcgtc acctcttatg
    19981 ttttatcttt ctaattcatt atcttgctca tctacaactt gtttcttaag atgtcttggc
    20041 gtcttttcta agccgtcatt gctcatttct atagtttctg tcttttcttt gtatatgatg
    20101 tttcctgttg atttatctag catgaaatcg tatctatttg gtgcctcctt tacttttaaa
    20161 tatgattcat gggggatagt gacttgataa agctgatttg caacttcaag ctctattttc
    20221 ccatcttcaa agcttgcgat ttcacatttg tttaattgtg tttccattgg ctctgtatgt
    20281 tctattttag ctatttgaaa acgttgatta atataattga agttttcttt attttgattt
    20341 gatttttgat aaataatgtc ttcatatttt gaaaatgcat aatcttttat ttcgatattt
    20401 tctacatttt tcatttgatt ttctacttct cttaaacctt tagtagcctt gtctaactca
    20461 taaaaaggtg aaataacatt tgctacactc tcttcaatca ctgctttgat gttgggattt
    20521 aggttttctt ttaaatgttg taagtcattt tgttctcttt ctgttagagc ttcaacattg
    20581 aaaagcggtt tatccgaaaa aaattctttc aataagtatg tttgataaat tttaccctgt
    20641 tcattttgat tcatcattta tagcctcctt tatttttagc gctctatctg attgtcagaa
    20701 gtataaatat caataacttt tttgttttgt gcatgtttaa tagactgcga gtctttctca
    20761 acagctctat ccactttaga tttagaactt ggatcttggg agacgatata tgtcttgacg
    20821 tactcgtaaa atgttggctg ttctaacatc gtttgctgag gtgcatagct aaatggtatg
    20881 ccttgtataa tgtttgtttt attgttttct tgatgcgcta acgtaggtgt aaattgacta
    20941 tacatttctt tagaaaaata attagcgtct tgtatggtat atagctgatc gtttctaaga
    21001 aaagtattgt ttttagtttt gtttggttta atcataactt catttggata tttgttaact
    21061 tctaaataaa tgtcattagc gtctttatcg ttaatggatt ttatcatttc actagatact
    21121 ttttgaacat ttttcatcaa catcatttta tcttcggcag acatattttt tgcgtatcca
    21181 tatatatact gtattgaatc atcattatta tctaatccca tatagctacc tacaattaac
    21241 gctgtcattt ctgcttgaaa ctcttcttct ttcattccca aagtcgttgg tgtttcaatt
    21301 attttatcct ttccatgtaa aagcgcatga cttaattcat gtatcatcgt tcttgtgttt
    21361 tctgcttgtt cattatcttt atttaagatg atttttttat ctccggttga cgtttctgta
    21421 tagtatcctc ttgcgccttg aatgctgtcc tcgctttcct tcacatctat attgtaagct
    21481 tcagcaatag cgtttaggca ctgtattttc tttgcataat cagttacttc tccacgtaat
    21541 tgttgaatca tttgagggta tttatcctca ggcatgtctg tttgtcgaat atcaaataca
    21601 ctgcctttta caaaaaccag ttgcttattt attttaattt cgcctttctt cataagtgct
    21661 ttctcttctt ttgttgcttt ctttattggc aaaacagatt ttttattatc tatgtctctt
    21721 acaaaggttt tcctttcaga tggtacaagt atcttaatag ctttttctcc cttttgtact
    21781 tgtgcgccta atgattgcca ctttttataa ggtgctattg cttttgcgcc tttgtattga
    21841 ttagcaatca ataattggtt tctaggggag taattataga attgagaaag aaagcttata
    21901 tattcttcta cgctgtctgg agaataaaaa aattcttcta atttttttaa agcttgttct
    21961 gaagtttctt caataaaatg gtttaactca gcctttttca ttgttcatca tctccaaaca
    22021 tcatattccc caactgtaaa tcaccttcat caaaatcaga catatcattc aaaattattt
    22081 tgtgaaattc aggactatat gaaaaatata ctttgtctat atcatctgtt ttttcgaaac
    22141 tttgtaacgt gtctttaata acttgataaa catcttcttt aataaataaa gttagatgtt
    22201 cattatcttc tgtttctatt tctgcgtaat gctttccttt aacgctaaat aattttacaa
    22261 tttcacaagt ttttgacgtt ataggcatat cttcaatggc gaaaaaataa tcttcgatat
    22321 tgtctatttc actttgttgc gtaacgtcat aatcattatc aatcacaatg atccctttat
    22381 ctggattgat aataaaatta agttcattat ccttgcttag cttttgataa cattgttcta
    22441 attgtgcatc tgtaagtctg aagtcatatt caaattcttc tgtttctacc gttataatat
    22501 tcccagccaa tttgacaaat gtagctggtt cagcatcact aattaaaaaa ttattttcca
    22561 cgcttaaccc ctcctatttt tattagtcat ctttctaatt cttttttctt cgatttgcgt
    22621 ttcaacatct tttttcgcac tttctaacca acttgtaggt tcgctatgtg cgcttctatt
    22681 ttcgtcaaaa ccttctaaat aggcagtcgt attcgcaaat tcaactagtg ttttaatatc
    22741 ctttgaattt tgattcatga ttaatgttat tttcttcata ttacttatta actctgatgt
    22801 ttcatcaact ctttctttca acattagata ttcattaata atatattcaa tagcttcacc
    22861 gctattttta aagtcatttt ctctttggat ttttgttaat aaatccaaat actttccttc
    22921 aattcttata cgttgttgaa ttttagacat atcatttaac tacctttctc tatatgattg
    22981 cttatcaaat tcttgatagt attgctgtat atttttttct ttatcatagt cactgcgcaa
    23041 tgcatatttt gttctatata gtgcttgatt aagacacttt cctattttta actttgttat
    23101 agttgcattc gattttgaat gtgtagattg ttgctttact ttcattaaat cttggtctaa
    23161 ttctttgatg ttctttaata ttgtattacc cgaacgggca tataaatctt tcatttttgt
    23221 tttttcatat gagccataat tagaattttc gccgtaagtt tctttgtaca aattggtttg
    23281 tctttttaag tcatttttaa aaacttcaaa atctttactg tgataatttt ttatatagat
    23341 atctgttatt ttatctattt ctggtctgat atatttcaaa atattattgt tataattcca
    23401 ctgattcctt gggatatctt ccaataactt atgaactttt tttatttgct ttttcatggc
    23461 tttgtcttta tatggttcag attcatcacg aataccttta actatccttt cgcgtataaa
    23521 ctcatttatt ttttgacgtt ctccctttat atcaaaaagc tcattggcaa aggtagattt
    23581 catatgtttt agtgttgctg gctttctttt tcctctagtg cgtttaatta ctttttctac
    23641 tgctgcaaca tgaacatgta tgttatccgt gttatagtgt attgcgccag accaaactaa
    23701 attgtcttca agcccttctt ttttctcaaa ccttttcatc attttccttg tagcttcttt
    23761 aattttttgt tcatctaatg tatgtgtttt tgaatcatat atacctacac tttctaaagc
    23821 tgaattatca aaactgataa catctttcca catcatacct ttattttttt gtgcatgact
    23881 aaaatcactt ttaattttat ttatcgcctt tttcgacata aagttatcat gttcattaaa
    23941 catattctga cttttttctt cgtcaaacat gtagtgtgcg taaacatcgt atttaaaatc
    24001 atctttattg ttttgtgctt ctccacgttt catataatta acataattac tgtattcata
    24061 aaattcattt ggttgttcaa attgagatac tgttacaatc tttggcatca tatcacctac
    24121 tcattgattt aatattctaa atcattaata gtgttataaa ctcggtctat tttatccgct
    24181 aattctttta aagctttcgt attttgtgct tgttggtgag attgttcggc atacaaactt
    24241 cggtaacatt ctaatggatc aatctgttct attatgtcca ataaaaattc gttccttgat
    24301 atgttttttt gctttgccat tcggtcaatt ctctttatag tttctacatc aacgcttctt
    24361 attttaatat ctcttgtttc cataattctg ttaacctcct aatcaaaaat acttaaaaat
    24421 cttttaaaaa caaaattttc gtaagagcat aagggaaata tgaccccata ttttatgggg
    24481 tggctggcaa agccagccat ttcaaggttt ttaaagaatt taaagtgacc ccacaataaa
    24541 ctatagtgtc acagttttgt gacactatat attgtgtttg tcacagtttt gtgacacaac
    24601 atgtagaatt tgtcttattt ttgtgacact tgttttttag cttattttgt ttgttgcttg
    24661 atatttttta ttcaacaaat cgattttttc ttgtagcttt ttctcagctt cttctacatt
    24721 atttatttcg ttttctttct cttttatttc agatttttta gcttctattt gattgtttgt
    24781 ttcttctttt tcatctgaaa tttgatatgt ttttttggca tctaacaaat taatttctgt
    24841 atttaaatct tctatatttt tctttaattc tcctttttct ttttccttat tttttaaatt
    24901 ttgtttatta atttcaatat catctttaat aaaagataac tggtaatctt ctttcgttgg
    24961 caatgttttt ttctgttcta ttgatacatt tttttcatta ccaaacactt ttaatatctc
    25021 ttcttcatta tctgattctt tatctttact taatatttta actactaaaa cttcaaaatt
    25081 aggtttgaca tcgtaaactc taatcgcttg tttattatcc atttgattta taattttata
    25141 tttactttca atatcaggtg tcgcattagg tattatttta tattgaacat tttgaataac
    25201 atcttttcct aattcttgct tatattcaat aacaaaatcc attgtttttt tctctttgtt
    25261 ccatgtccat tggttaattc tcacactgcc agaagtagat gtattttgcc atttttttat
    25321 aggtgtatga acattagtta ctgggtcttc tgtaaaaaaa cgactaccta agataagtac
    25381 aaatgaacct acaattaaga cagttagcat atagaatagc caactgcctt tgtttcttat
    25441 agaagttttt ccaggtataa aagctctaaa taactctttc aaaataccac ctctttaatt
    25501 actttcttct attttttctt catcaaattg tttaaattta cttactttcc aaatgttatt
    25561 gccttcttgt ttaagcgtta attccgaagc aatttttctt ttgtctatac catctgaact
    25621 gccttttata gcttgagtta acgtcaaata catatgatat tctttcttct caggagaata
    25681 tctttcatta taaattttaa catcttgtat atctgtttcg tatttaatag gtgaacgtct
    25741 tgtctctcca aaatactgat cagcaagtga tttatccatt aattcttttg ctttttcata
    25801 cctttttttg tcatctgttt tagtacttgt cacatataat accttcacaa attcttctgc
    25861 ttttcctcta agttcttctt cttccttaac aagattttta ttatctaatc tctctattaa
    25921 cttttgtttt tcatcattgt ctttttgtaa actctttatc tttttacttt gtatttctac
    25981 agttttattc aattcattgt ggtcttgaaa aacaaaataa attataagac ctattacaga
    26041 taataatata attacggtgc caaatagttt accattttta ttcgcatgct ttttattttt
    26101 agacattatt ttacacctct aatattctaa tatataaatg tttcatcgcc gtctaatttg
    26161 aaagttcttt ctccataagt aaaaggaata atgttatatt cgcttactct tatttcacca
    26221 tttttcttta cttcttctac aaaagcgatg tgaccatatg gcgttgaacc aaatgctttt
    26281 gaattgtatg gccaagatat catagcgccc actttagggg tttttcctat tttatatcct
    26341 tgtgcttttg ctttgtctgc ccaatcaccg ccattaccaa aagatagctc cacaggcaaa
    26401 cctaattctt ttctacggtt atagacatac caagtacatt gatgataagc atatgtgttt
    26461 ggttcaaatg ttttctttcc atattttggc aacttcccac catgtttttc aagaactttt
    26521 ttcaattttt cgatagattc tcccgctggt atgtcgttgt cgcaactaac cgttgtaccg
    26581 cctttttcta cttctcctaa tgatttcata ttacttttta cagttggaac ccaacgcgca
    26641 ttcatattac ttggatcatt agcagcacct acaggtgcat atttagatcc tattttttca
    26701 ggtgttgtta accctttaga gatgtataag tcatataaat ttcttgcgcc atcttctaat
    26761 ccgtcttcta tagttttata tgttgaatct tgtattgatt tgttacccat tacagatagt
    26821 gggttgtttt gtcttgtcgc atttgcgcct tttccccatc cggattcaga agctataatc
    26881 gccataaaaa gtgaaggtgg tattttgttc tttttagcaa tttcaacaat tttttctgac
    26941 ttaccttcta acgcgccacc tttagcgttt ttttcaaatg tttgtagtcc tccttctttt
    27001 aggttacctc ctacaacttc acaacctttt gacgatgatg tagcttgctc ctgtttgtcc
    27061 ttttctactc taaccgcagt tataattgtc ccaacaataa caatcgttaa taaaatagaa
    27121 aataacacag cacccaataa taaccatatc ttccataaag caatattcgc tttatgagca
    27181 ataaatttta cagcttgttt ggctgctctt tttgctatag tgcgcttttt caatattacg
    27241 cacccccacc aaacaagtca ttctcttctt cgctaatatc aattttaaac tttacattgt
    27301 ttactccagc aatatttaaa ataacttcac ctgttcccaa ctgagggatt acacttaact
    27361 cagaatcact caactcttgt gaaaatacat tacctaattt ttcaacattt gaagtgtcct
    27421 gtcgcataat gaacctatac gttgtttgag aaaacaatgt cttcactttc gcaacattct
    27481 gttcttgtga atcctctgga ataaagtctt taatatcttg agaagcaaat ataaatccag
    27541 caaaatactt tctatcttct cgaataaatt tttcgcattt ttcaactaca ggtgcatctg
    27601 tttcacttgc agaaattaaa ttatgcgctt catcaaaaat caatctatac cttaccgcat
    27661 cctctaattt taattcgcgt ctgttataag cttgaaattg aggagagcca aagtaaacca
    27721 tttcatcaaa caacaagttc attacgttaa ataattgtgc cttttttact tcgtcaccca
    27781 ttgcatttaa ttgtctaata gagaagttaa taatagagaa ctgcttaaag ttttcaatcg
    27841 ttgtatggcc atcaaacatt gcgccattag atgaaataat atcttgaagc gttaactcta
    27901 ttgattgata ttgtttcaac ctatcctctg atatatttgg tctcacattt ttaaactctt
    27961 gatcttcata tactaattcg cgtaagtact gtaacaactc tgaaaaagta ggatagtctt
    28021 caggatttaa gttagtgata ttggtttgct tatcaggatt agcactccat aagccaatat
    28081 ctttgtaaaa ttggtctata acaattctga aaagctgctt atcttttgct ttggcatctg
    28141 gattcatcaa tccataaaac gtacttaatt tatgcttatg ctggttaaaa gatgtgactt
    28201 catcgatttc ttgcacgttt ttgcctaaat catcaacttt ttctcttaca gctactgcat
    28261 ttacttctag cgggtttatc atgccgtctg taccatctaa agatatggtc tttcctccta
    28321 gaacctctac caattttaca aattctcctg tagaatcaat tgttctcatt ctgtggcctt
    28381 gtattgcctc ttctaataat atcttcttaa gagcgtgaga tttccctgca cctttggcac
    28441 ctacaaaaac gccatcatat gaagtacgtc tactgtcttt atagaacaaa tcaagcaaga
    28501 tatttcctcc cgtatcagat tctccgaaat acacaccgtg ttcatcattc aacgatactg
    28561 aatgaaacgg cacgcctatt gctattgttt gtgacggtat atcttttttt attcttggta
    28621 gtacagattc ttgttgtgtt aagctcatcg taagtgcctg actttcaaat ttttgctcat
    28681 taagataaac agtgccttta aagtccattg cattaagaaa aacaaaaatt tcatttaatt
    28741 tttcgtcgac ttcatctttt gtccttccac tcacatacag tcttataacc acttctttta
    28801 tcgactctcc accatttcga acgtttctta ataatgtatt gagttcttga tacgattctt
    28861 cagcttcagt tcttgaaaca acatctctat cattatgcat acgatccaaa tattctgtca
    28921 tacctgtttt taaattgttc ttgatttctt cttttgtttt tgtcctcaaa tccattgtaa
    28981 taatggtatt atccatagtc atgatttgtc ctaaccaaaa atcttctaca tcagttggcg
    29041 tctcatagat agttatacaa gtctcatatc ccgtgccttt tcttacatag aagtcatttt
    29101 taaaattaac gccgcctctt ggttgtatct gagagagtaa ttcaatatca aagtcagatt
    29161 gtgatagtgt gccgctatca atcgccttct taacttttct tcgttctctc catctgctat
    29221 acacactact catgtgaaaa cccccttaag tattacttaa tttcttcgca ttgattattt
    29281 aacttataaa gtatttttat ttcttgttct ttcgttaatt ctttaattgg aaaacctcta
    29341 gccattgact ttttatatgt ttgaatgttt acattaagtt cttgtgctgt tttgccaaat
    29401 aacattgcta aaaaagaacg ttccattctt gatgactgtt ctaaatattc cagttttttt
    29461 atcctctgtt ctaagaagta cctgtatata cctacttctt ctttctcata ttttctgatt
    29521 aaatggcttc tttgcgtttc tgtatctatc ggcatattta acgcgataat cttattgtct
    29581 ttgttgtaag ttttaagcat gttaagaagc acgcccatat catataattg ctcatgtgaa
    29641 tttctactca taatatcttt tggattcact tgaaagaatt tcatataggt gccatctcta
    29701 agtacatata agccaccttt atggtcttct gtccttctga tgatattaga tatatccaaa
    29761 aaggtttttg atgctctata aggcttaaaa agctttatag gcctattttt gtgttcaact
    29821 ctttttttac gtagtttctt ctttttgatt ttggtttcgt taatatctcc aaaaatagtt
    29881 gaagttgatt catctttttt agaagatatt ttaatagttt cttcctcaaa aactttacca
    29941 aataaagaag aagcatcatc tttttttgat gatgcctcct taaataaagt tttattagag
    30001 gaagtctttg tagtttcttg tggcgttttg aacattgcca tgatttgtga cctccttgtc
    30061 tttttcaatc atttcatatg gaatttctgc ataaacattt ctatcttttc ttacggtaat
    30121 ccaaaaaacc cttagacttc tccgtttagg atttgttgca aatggacgta ttattaaagt
    30181 taatataaca ataagttgta agatgttcat atacaaagtg gcgctttcag gtacaattcg
    30241 aattgcttga agaatataac tcccaacaaa tactattaaa aacgtcataa catctttcat
    30301 ataaagccaa cgcgtaattt tagcttctac gtttgtattt tctggtattc tattggacat
    30361 caaaaacctc ctaacgtttt ggttttatat ttttagagct atttttatta ataggtttat
    30421 tcacatgtga ttttggaatt gatttattta caggtttgtt cgtttgtacc ggcttcacat
    30481 tttgtacatc tttataactt tttggagatg ggttattttg acttgtgttt tgttgttttg
    30541 aaaaaccatt atcagttatt tttctttgcg ctttaggcgc attcataggg ttagatttag
    30601 gtgtattagt attaagttga ttagtctttg gtgtgttaga tttttctcct tgtgttcgtt
    30661 tgataacatc gttaagcgtt tctttctttc gtcctccgtc tggagctctg tttgcccata
    30721 gttgcgaatg actttcgcca acacctttga atgtattatt tccgtaatta gaatttttca
    30781 tctcttcgtc aagagactgt ctgttgctgt tagattggct gttatgacta ctgtttgatt
    30841 ctgtgttgtt ttgcctatcg ttaacttgac tattactatc tatattctga ttatctttaa
    30901 cattttgttg tttgtctgta ttttgattat tcgaggcctt attgagttgt tccgacttag
    30961 attgttgttc attgctgtta ttattaacgt catttctttt tgatttagaa aaatcatctc
    31021 tcactgcttt aatcatacta cttgttccat tagccgtatt tgtcgctagc gcagttgccg
    31081 ccttacccat tccatacata ccggccatcg tgtaaaacgg tgatctaaga ccggcatcat
    31141 aaccaaattt acgctcaatg gactttggac catctatcat aaaccatgtc gcagcaataa
    31201 gtcctaccaa ccatatacct gttggcttat cgtttaagaa actaacaact tctttgtata
    31261 agaacaaaca gaagaaaatc ataataacac ttacaaaacc gtttaaaata tcgataattg
    31321 ttgatttaag gcgttctcct tgtagtgcat gtgaaaagaa aatagctaaa attttattta
    31381 caattatctc taaggcaata cttgcaacac gtaatgcagc taaaaagtga cccaaagcta
    31441 aacatataag ccccacagca gcagtaaacc acttcacatg aaaacgataa tatttttcgt
    31501 cacctaggcc aaaccagttt ttgtccaaat cttcaacttt ttgtcgacct gcttcatcga
    31561 cgactaactt tttcttaagt atctctttag actctttctt tttaacttct ttttcctcta
    31621 attcttggtt aatgttgata tgcttaattg tgcttgcatt aaggttgtta tatttttgat
    31681 tgtttcttgt cgttttaaca tctggaaatc ctttttccgc catcaactgg acatctacaa
    31741 tgcttttagc aagcatcgaa gaacccactt tagttccgtc tgtttgtttt ttactatctg
    31801 gatcaaaatt ttctatagct ttacttgctt cttttgttaa ttcagagcct ttttgtaaac
    31861 ctatattcac actacctaaa aacaaaactg caacaaccaa tgtagcaaaa atatcagctt
    31921 tattaattgg tcttcctaac attaatttga tacctatata aatgatggcc aacgctaata
    31981 ctgaaattaa tactggcttc gcttcaagaa ttaatctgct aatactgtct tgcgtaagta
    32041 atccaaaaag tgtcaaaact tgaatagtaa gatttgaaac taaatcagta atccaaacaa
    32101 gaaattgtaa gaagtaccaa cctatccagc gtaacatgtt aggtacaatt tcagacattt
    32161 gtaaaacatc ttgatatttt tctaatacct tctgtgttgc actatctctc atcttaacta
    32221 cctcttttct attccttcaa tgaacttatt taattttgaa attgccaact caatgttgga
    32281 cgtgtctttc actgctaaat cttgataatc catatcatct attttcttgc ctttttttgc
    32341 gaaatataat cgtgaagtat cacctaaaaa ctccccattc tcattccaat ctttttgatg
    32401 cttatcgttc ttgacattat aaattgtgta ataaatttta atcccttgtt cttctgccac
    32461 tttttctaga atctttttag aattataaat atgttcttct ccagccctac tcaaatcact
    32521 aattgcgata acatattcat tcttaggatt ttttaattta tcatgtgctt ccttaggtga
    32581 tatttcttct attccagatt ttgaacaacc cgacaaaagt aatatcacgg ttagaaaaat
    32641 tacgaatcca actatatttt tcatgttgtc tttgcctcct tgataaattt ttgaatcatt
    32701 tcaatacgct tagtcttttc ttcttctttt acctcattat cagctgagac cagttcttct
    32761 aatttttttt gcgttatagt attaacattt ttaccttgtt ctgcatatgt tcttaagtat
    32821 ctttcgaaac gtcttgtaat tttgtcaaaa atagcgtctc ctactttttc tttcaaggtt
    32881 tctaaacgtt taactcgcac ggcaacattg ccttcttcat tgctttgttt atattggtta
    32941 atgtcattaa tatttttatt ttcttgttct ttttgaccgt ctatcccttc ttgcatttct
    33001 tcaatacgtt tatcccaatc aactaatgtc gcttcaagtt gtaaatgcct gtgctcacat
    33061 cgcgctttta acataggtct aaaatgaata atactttggc ttgtatccat taagtctgat
    33121 aagtactggt aacgatattt cattgcatac ttgccgtcgt tgaaaattgg ataagacatt
    33181 actttgtttc gttttttatc ttgtcgtttc aagtttctaa caacgactgt ctcaccttct
    33241 tgtaagcgcg ctaattcatc tggtcgtagt aacggtcttt cttcttgatt ctcagtaatg
    33301 cttgacttaa ttgctcctgc ctcacccgaa cgactatgaa ctgtgatttg ttgacttcca
    33361 atcattgcgc taaaatcttt taatgtctgt tcttgcgtag ccatgatata caccttgttt
    33421 tggcaagcgt ccataatcgc atcatatgca tcaccgtatt tatctttaaa ttgagaaaat
    33481 gcttgaacaa agaaattaaa acgtaatcct cttgaaagag atacgttcac tttttgagat
    33541 aaattcggta tcggaggcat attacctgct tcatcaaggt ggaaaataac gcgtcgatac
    33601 gttgattgat tttccgtata tagctgagaa tttttcgaaa tttcaaaaac aatttggtta
    33661 acgagtatag acgctatacc atggttagca ttgttataat ctggtacaac cataaatacc
    33721 gctattggtt ttggaaaact attgaacttt cttatcgtaa tatctgagtt atctaattcg
    33781 cctttttgat taaatgtgac ctgtccttta tcatctatat gatcaacata aaaataactt
    33841 gtgataactt gattatttcc gtctttctct attgtttcta ttctatctcc gactttaagt
    33901 actgttttga acggatattg ccaataccca tattcattta cacctatttg aatgccttct
    33961 acttcttcgt attcaatgcc gttttcacca tctcgtaaca cttgtacgcc caattcaaat
    34021 gtactatctt tggttgcgta accatcaata atacttggaa atcctaattt attaatgtca
    34081 aaagaggtgt gacttgttaa tttggctatg gtgtcagttg taaatggttg taatgccttt
    34141 aaagccgttc cgatgataga cgaacgcatt ttgtctggtg cagcttgaac tgtcgcatat
    34201 tgcgttttag ctggactatg actagggaac ctttccatat actcatctaa tgccgaaacc
    34261 tcaattaatt gaactttgtt tccaattgca atttctttat aaaacctttt tgtgcttaat
    34321 gtttgtaaca tattagacac actataaagt gtgacttttt ctgggttctt cggtagggtt
    34381 tcttcacata gtgcaagtat catagcgtta atcaatgcac cagccatcaa gttccattcg
    34441 ttgtcttcta caccttcgtt attggttaac gtatatgtaa tttgctttgt catctcttgt
    34501 gcttctccca aatcgccttt catatattca tcaataactt gttgcaatgg gttgaagctc
    34561 atcgttgact ccataggttg atccaaatta aagatctcaa cttggtatcc tctattttcc
    34621 aacgttttat acgatgcaga aaaaagttct cccttcatgt catttacaat taatgatggt
    34681 tgttctgaag atcttgaata gttttctatc attgataaaa caacaatttc tcccttacca
    34741 gaacgagatg tacccacaat taaattattg gttggtgact catcaataaa tgtaaattcg
    34801 cgttttgaga aaaatgtagt agcgaattct ttattcattt ttcctaattt atctttgcgt
    34861 aattctaatt tttcatcagc tgttaaatca gtaattccgt taatttctct aatagcagtt
    34921 tttctttgtt ctaaatattg cttcgtttta gtcacaaagt catatacacc aaacattgcg
    34981 acaggcaaac caccttctcc ttcaaatgca tgctcccttt gtggtattgc tttatactgt
    35041 tgcgtaattt cctttcgtgt ggcaaatcgc cctgacgctt tttcgttgtt gtttagtgat
    35101 ttgaaacgct gtctaaattg atatactttt tttccaaaaa atataaagac gagtacccaa
    35161 attggtatat ataacaacac agcatctaaa cgaaagttaa atatataatc tgtatatttc
    35221 gtgttcagtt gtttcatatt tgtgaaaatg tcatgatttc caaaaaacac tgtcattaag
    35281 atgttaatca aaaaatttgc aattgcagtg gccaataaca taattaataa taatatgctt
    35341 accaatatac atggatgtgc aagcaaatgt ttattttgag aaaacgataa ctttttcctt
    35401 ctatagccat gtttgtcttt tgaaccaaac ctcattataa aatcacctct taaaatggca
    35461 agtcttcagt aacgaaacct tttgctttct ggaaagttgt gttattttca ttcacttctg
    35521 tatgttgttt atcatcaaat gttaagttat ctaaattatc agtgctatgc tctaattcat
    35581 tttctgattc ttctactttt ttattactaa gatacacaat acgaccagca acaaggttca
    35641 tataatattt atcaacgcca ttaacacttt ttttgttcgt gcttacataa ccttcaacat
    35701 aaaccatgtc gcctttttga ttatatttca ctaaattttc tgctgtttta tcaaaagccg
    35761 ttatgtttaa aaagtcagtg ccatatgcat tatttactgc tagtgtgaag ttacatctcg
    35821 cttgcatgtt ctctttagtt gttagttcta agtctctagc aatacgtcct ataaatttaa
    35881 actcatttag cattgtttca cctctattta tttaagttct tttttgaatt tttccaaatt
    35941 ctcgtttgct tctttaagcg ctttttcttt attttcaata tcagatttct tttctttttt
    36001 atctttcgct ttctttaaat catcttcttt actcttaacg gcttcttcta gccctttcaa
    36061 gcgcttgttt tgttcatctg caatcatttt tgtaacttct ttgtcttccg ttgtcgctgc
    36121 taattctttc gccttattta aattgtctgt ctctatatac gcttttacca gttgtttttt
    36181 tctattgttt gttttatcga taccttgact gtaattaatg gcatcagaaa acttattctg
    36241 gtgtatagct aaatcaaact tcccattatt agttggatat ttcttattaa attcttctaa
    36301 agcgctataa tagtctctgc taattaaata cgcttctaca aattttgcat cagacaactt
    36361 aaccgcctta tcatactctt ctttttgagc taataattgc gcatatattt gtttgtcttt
    36421 tttggttaat gtctcttggt ctaatttagc aaaattatca atggcttttt cttcttctcc
    36481 taaaagagct tgttggtata tttcttcttt tgtcgcaacg tcagatgctc cttttttgtt
    36541 tggatctgta acaacaagta tatatactaa caacattaca aaaaaaatca gccccaaaaa
    36601 cattaatccg cgctttccct ttaatttttc aagtatttct ttttgcttag ttttatttgc
    36661 attgttttgt ctttggtatt gaaattttgt attgtctgga ttatttttct ttttattcat
    36721 agcaactttt ttgtcacgat atgacttatc cacttcctgt agggcaatta agtactgtcg
    36781 tttcagtctt ttcaattctt ctgtgtcagg cgcagttgct ttaagcaatg tataaatttg
    36841 ttgttgtata ttaatataac cactattcac tttgtacgtt gactctaaaa tatcctcata
    36901 tgtttgttcg tcatacatta cgtaatcaaa tttatctagt gattctgtaa atttccctgc
    36961 ttccatatct tctgtatagg acatcatttc ttctaatgaa aaatcacttt gatttttgat
    37021 gtgaaacgat ttcgttttta ttctcaacaa gttttatctc tcctttat
//
LOCUS       DAKTIO010000009        35074 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.009, whole genome shotgun sequence.
ACCESSION   DAKTIO010000009 DAKTIO010000000
VERSION     DAKTIO010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 35074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35074
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.009"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            208..777
                     /gene="ahpC"
                     /locus_tag="O0293_000317"
     CDS             208..777
                     /gene="ahpC"
                     /locus_tag="O0293_000317"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HCY0279229.1"
                     /translation="MSLINKEILPFTAQAFDPKKDQFKEVTQEDLKGSWSVVCFYPAD
                     FSFVCPTELEDLQNQYEELQKLGVNVFSVSTDTHFVHKAWHDHSDAISKITYTMIGDP
                     SQTITRNFDVLDEATGLAQRGTFIIDPDGVVQASEINADGIGRDASTLAHKIKAAQYV
                     RKNPGEVCPAKWEEGAKTLQPGLDLVGKI"
     gene            793..2316
                     /gene="ahpF"
                     /locus_tag="O0293_000318"
     CDS             793..2316
                     /gene="ahpF"
                     /locus_tag="O0293_000318"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCY0279230.1"
                     /translation="MLNADLKQQLKQLLELMEGNVEFVASLGSDDKSKELKDLLTEIT
                     DMSPRLSLSEKSLKRTPSFSVNRPGEETGVTFAGIPLGHEFNSLVLAILQVSGRAPKE
                     KQSIIDQIKKLEGSFHFETFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREE
                     SENIMAVPAVFLNGEEFGNGRMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASG
                     SAAIYTARKGLRTGIVADRIGGQVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDID
                     AMTGIRATDIEKTDEAIKVTLENGAVLESKTVIIATGAGWRKLNIPGEEQLINKGVAF
                     CPHCDGPLFENKDVAVIGGGNSGVEAAIDLAGIVNHVTLFEFASELKADNVLQDRLRS
                     LSNVDIKTNAKTTEVVGEDHVTGIRYEDMNTGEEHLLNLDGIFVQIGLLPNTSWLNDA
                     VELNERGEIVIDRNNNTNVPGIFAAGDVTDQKNKQIIISMGAGANAALNAFDYIIRN"
     gene            2446..2688
                     /locus_tag="O0293_000319"
                     /pseudo
     CDS             2446..2688
                     /locus_tag="O0293_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2761..3387
                     /locus_tag="O0293_000320"
     CDS             2761..3387
                     /locus_tag="O0293_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDxxF motif lipoprotein"
                     /protein_id="HCY0279231.1"
                     /translation="MKKRLLLSTFLASTLILTGCASDQSDNEDHHTSTGIHAPKSAKK
                     LETKDIFNSDKKNSDISDAEMKQAIEKYLSVNSDILDNKYIMQHKLDKQIDSQTKVTE
                     KQAETLSHLSNLAVKNDLHFKKFVTENNIPKEYKKPVELMMNYFKALNSTIANVDEDI
                     EKLSYQPQNKINVVDVPTKYAGDVNKKQQDKIKDFLKSKGIKSDVIDK"
     gene            3642..4025
                     /locus_tag="O0293_000321"
     CDS             3642..4025
                     /locus_tag="O0293_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279232.1"
                     /translation="MKVRITFIILAILSTIVCLFLAAMHPTGPNTVTFEQPYLFTLNI
                     IIMVLVALPSLVLAIYDYMSFRISSAILQFLGAISWFFLSLILSLTQFTPFTLASFIT
                     SIILFTSTIITLAIGGKSVEKNDSP"
     gene            complement(4091..4672)
                     /locus_tag="O0293_000322"
     CDS             complement(4091..4672)
                     /locus_tag="O0293_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCY0279233.1"
                     /translation="MTIYLVRHGESKSNYDNKHFRSYFCGQLDVPLTDTGTKSADDLC
                     DYFKEKQIKHVYVSDLLRTQQTFEHIFPYDIASTTTPLLRERSLGVFEGEYKDEISAN
                     PKYDKYFNDPNFKDFRHSFSQKAPEGESYEDVYQRVEHFMNHVVNEDTQKDDIVIVAH
                     QVVIRCLMVYFNKVSRAEAVDLKVENCKPYIIE"
     gene            4850..5059
                     /locus_tag="O0293_000323"
     CDS             4850..5059
                     /locus_tag="O0293_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279234.1"
                     /translation="MTTQSFIFMNIILLDGATQRHPFFICQILAFLFPKFPHINLLLF
                     NPILSTFSNIIIHIDHFHANKKDDN"
     gene            complement(5098..5349)
                     /locus_tag="O0293_000324"
     CDS             complement(5098..5349)
                     /locus_tag="O0293_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCY0279235.1"
                     /translation="MFGFIGMLIVGGLIGWAAGAIMGKDIPGGILGNIIAGIIGSWVG
                     GKLFGQWGPELGSIYILPALIGSIILIAIVTLILRAMRK"
     gene            5649..5912
                     /locus_tag="O0293_000325"
     CDS             5649..5912
                     /locus_tag="O0293_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0279236.1"
                     /translation="MNMHILYNLRTKHNLEIDELAQQLNEKYGTKYEAHQIWEWENHH
                     HEPKFKDAMHLADFFDAPYEMFLESKVKEYQKHLEEVDIRMDK"
     gene            complement(6050..6622)
                     /locus_tag="O0293_000326"
     CDS             complement(6050..6622)
                     /locus_tag="O0293_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCY0279237.1"
                     /translation="MKLKSLAVLSMSAVVLTACGNDTPKDETKSTESNTNQDTNTTKD
                     VIALKDVKTSPEDAVKKAEETYKGQKLKGISFENSNGEWAYKVTQQKSGEESEVLVAD
                     KNKKVINKKTEKEDTMNENDNFKYSDAIDYKKAIKEGQKEFDGDIKEWSLEKDDGKLV
                     YNIDLKKGNKKQEVTVDAKNGKVLKSEQDH"
     gene            complement(6803..7171)
                     /locus_tag="O0293_000327"
     CDS             complement(6803..7171)
                     /locus_tag="O0293_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCY0279238.1"
                     /translation="MKTILKTITYLVLTIIGAYAALFILKTIDSHGITDQFNPLVKKD
                     DSYVKTTEVSTRMDDQLRSYSQSVFNKEGKETQLMYTATFDVKPHRYLKITHKGHHVE
                     TFEEVEKEEVPKKALDKLSR"
     gene            complement(7540..8151)
                     /locus_tag="O0293_000328"
     CDS             complement(7540..8151)
                     /locus_tag="O0293_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCY0279239.1"
                     /translation="MFKKYDSKNSIVLKSILSLGIIYGGTFGIYPKADASTQNSSSVQ
                     DKQLQKVEEVPNNSEKALVKKLYDRYSKDTINGKSNKSRNWVYSERPLNENQVRIHLE
                     GTYTVAGRVYTPKRNITLNKEVVTLKELDHIIRFAHISYGLYMGEHLPKGNIVINTKD
                     GGKYTLESHKELQKDRENVKINTADIKNVTFKLVKSVNDIEQV"
     gene            8351..9058
                     /locus_tag="O0293_000329"
                     /pseudo
     CDS             8351..9058
                     /locus_tag="O0293_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505297.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            9097..10035
                     /locus_tag="O0293_000330"
     CDS             9097..10035
                     /locus_tag="O0293_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HCY0279240.1"
                     /translation="MLNFDEQIAKLKQMNIFFNIIDTEKANEILRKNNYFFKLAYFRK
                     NFEKKNGGYFIEFAYLSDLATIDMKLRYTMLHLTLDIEHSLKYLVLKLITENNQEDGY
                     KIIDEFLCIDKSYSNSNFDTNSRTPEEVMETKIKNKNEIFKHMNKRGQLPEKLNKYYQ
                     NPPAWVCIEFMQLGQFVSFLNFYYKKYNDEELRVANILMPLVKNIRNKSAHNQPIIAN
                     LNYDSRLPQYLFEKGNNIGISRNMFGIKNFIDTFATLELHNQVCSNAIIQARYHDLDQ
                     LQKRYKRNESYYNNALAIKRFFIALDKIIDFNRPKV"
     gene            complement(10301..10543)
                     /gene="rpsR"
                     /locus_tag="O0293_000331"
     CDS             complement(10301..10543)
                     /gene="rpsR"
                     /locus_tag="O0293_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002484306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCY0279241.1"
                     /translation="MAGGPRRGGRRRKKVCYFTANGITHIDYKDTELLKRFISERGKI
                     LPRRVTGTSAKYQRMLTTAIKRSRHMALLPYVKEEQ"
     gene            complement(10595..11098)
                     /gene="ssb"
                     /locus_tag="O0293_000332"
     CDS             complement(10595..11098)
                     /gene="ssb"
                     /locus_tag="O0293_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCY0279242.1"
                     /translation="MLNRVVLVGRLTKDPEYRTTPSGVSVATFTLAVNRTFTNAQGER
                     EADFINCVVFRRQADNVNNYLSKGSLAGVDGRLQSRNYENQEGRRVFVTEVVCDSVQF
                     LEPKNAQQNGGQRQQNEFQDYGQGFGGQQSGQNNSYNNSSNTKQSDNPFANANGPIDI
                     SDDDLPF"
     gene            complement(11119..11415)
                     /locus_tag="O0293_000333"
     CDS             complement(11119..11415)
                     /locus_tag="O0293_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCY0279243.1"
                     /translation="MRTYEVMYIVRPNIEEDAKKALVERFNGILATEGAEVLEAKDWG
                     KRRLAYEINDFKDGFYNIVRVKSDNNKATDEFQRLAKISDDIIRYMVIREDEDK"
     gene            11659..11850
                     /locus_tag="O0293_000334"
     CDS             11659..11850
                     /locus_tag="O0293_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279244.1"
                     /translation="MNLNIDWSKDFQEFQEILNSGIHPEWLYCAKANLVLEPAYTGEG
                     KQFFSTQDIINASKIIPFF"
     gene            complement(11936..13033)
                     /gene="ychF"
                     /locus_tag="O0293_000335"
     CDS             complement(11936..13033)
                     /gene="ychF"
                     /locus_tag="O0293_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCY0279245.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGALAANYPFATIDPNVGIV
                     EVPDARLLKLEEMVQPKKTLPTTFEFTDIAGIVKGASKGEGLGNKFLSHIREVDAICQ
                     VVRAFDDDNVTHVAGRVDPIDDIEVINMELVLADLESVEKRLPRIEKLARQKDKTAEM
                     EVRILTTIKEALENGKPARSIDFNEEDQKWVNQAQLLTSKKMLYIANVGEDEIGDDDN
                     DKVKAIREYAAQEDSEVIVISAKIEEEIATLDDEDKEMFLEDLGIEEPGLDRLIRTTY
                     ELLGLSTYFTAGVQEVRAWTFKQGMTAPQCAGIIHTDFERGFIRAEVTSYDDYVQYGG
                     ESGAKEAGRQRLEGKEYIMQDGDIVHFRFNV"
     gene            complement(13045..13248)
                     /locus_tag="O0293_000336"
     CDS             complement(13045..13248)
                     /locus_tag="O0293_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HCY0279246.1"
                     /translation="MASKYGINDIVEMKKQHACGTNRFKIIRMGADIRIKCENCQRSI
                     MIPRQTFDKKLKKIIESHDDTQR"
     gene            complement(13278..14159)
                     /locus_tag="O0293_000337"
     CDS             complement(13278..14159)
                     /locus_tag="O0293_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HCY0279247.1"
                     /translation="MNQVMNIISSLFEPLTKIETYENIATKIAMIVIYIIVALIVIKI
                     LNKMIEQGFKIQNKSKKSNKKRSKTLISLVQNVVKYIVWFIVITTILSKFGISVEGVI
                     ASAGVVGLAVGFGAQTIVKDVITGFFIIFESQFDVGDYVKINNGGTTVAEGTVKSIGL
                     RSTRINTISGELTILPNSSMGEITNYSITNGTAIVKIPVSVEENIDNVDKKLNKLFTS
                     LRSKYYLFVSDPVVIGIDAIEDTRVILRISAETIPGEGFAGARIIRKEVQKMFLQEGI
                     KTPQPIMTAYNHSENGV"
     gene            complement(14316..15161)
                     /locus_tag="O0293_000338"
     CDS             complement(14316..15161)
                     /locus_tag="O0293_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCY0279248.1"
                     /translation="MSELSKSEDQRITKTKDEQIKQIDISDIKPNPYQPRKTFDENHL
                     NDLADSIKQYGILQPIVLRKTVQGYYIVVGERRFRASKIAGLKYVSAIIKDLTDEDMM
                     ELAVIENLQREDLNAIEEAESYQRLMTDLKITQQEVAKRLSKSRPYIANMLRLLHLPK
                     KIADMVKDGRLTSAHGRTLLAIKDEQQMLRLAKRVVKEKWSVRYLENHVNELKNVSSK
                     SETDKVDITKPKFIKQQERQLREQYGTKVDISIKKSVGKISFEFDSQEDFVRIIEQLN
                     RRYGK"
     misc_binding    15390..15759
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15825..16928
                     /locus_tag="O0293_000339"
     CDS             15825..16928
                     /locus_tag="O0293_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCY0279249.1"
                     /translation="MKDTQLAQITLTDDSTGAIANPIHLSTAYKHPKLGQSTGFDYTR
                     TKNPTRSTFETCFAKLEHGIASFATSSGMSAIQLICNLFKPHDEILVSFDLYGGTFRL
                     FEFYEQQYDIKFKYVDFTDYEQVEKEITDKTVALFIEPISNPQMIAIDVKPYYQLCKA
                     KGLLSIIDNTFLTPYLSTPLAEGADIVLHSATKYIGGHNDVLAGVVTVKDESLAQQLF
                     DFHNMTGATLSPIDSYLLLRGLKTLHLRIERAQSNARKLAKKCQSLQAIDEVLYSGQT
                     GMLSLRLNKAYSVAKLLENLDICIFAESLGGTETLVTFPYTQTHVDMPDAEKDKRGID
                     EYLIRLSLGVENYEDIERDIIQALDKAQIGEIV"
     gene            16925..18085
                     /locus_tag="O0293_000340"
     CDS             16925..18085
                     /locus_tag="O0293_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HCY0279250.1"
                     /translation="MTLSKETEVIFDWRRGVEYHSANPPLYDSSTFHQTSLGGDVKYD
                     YARSGNPNRELLEEKLARLEQGKFAFAFASGIAAISAVLLTFKSGDHVILPDDVYGGT
                     FRLTEQILNRFNIEFTTVDTTKLEQIEGAIQSNTKLIYIETPSNPCFKITDIKAVSKI
                     AEKHELLVAVDNTFMTPLGQSPLLLGADIVIHSATKFLSGHSDLIAGAVITNNEAISD
                     ALYLIQNGTGNMLSAQDSWTLAKHLKTFPIRFKQSVENAQKIVSFLIKQDEISEVYYP
                     GLTTAHLEQAKNGGAVIGFRLADESKAQQFVDALTLPLVSVSLGGVETILSHPATMSH
                     AALPEEVRQERGITFGLFRLSVGLEDPDELIADIKYALKEAFNESIPHTIER"
     gene            18054..19895
                     /locus_tag="O0293_000341"
     CDS             18054..19895
                     /locus_tag="O0293_000341"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HCY0279251.1"
                     /translation="MSQFLTQLKDNVLVADGAIGTILYSEGLDTCPEAYNLSHPDKVE
                     RIHRSYIEAGADVIQTNTYGANFEKLKRFGLEDKVKAIHQAAVRIAKKAANKDTYILG
                     TVGGFRGIKQEDISLQTILYHTEIQIDTLIEEGVDALLFETYYDLEELTNVISRTRKK
                     YDIPIIAQLTASNTNYLVNGQAINEGLKQLVQCGANIVGLNCHHGPHHMQESFTHIEL
                     PEHAFLSCYPNASLLDIENSEFKYSDNAQYFGQVAQNLIREGVRLIGGCCGTTPEHIK
                     FIKESIQTLKPVNDKKVIPIPTKALFNPSQNKVRQSLTSKVQERPTVIIELDTPKHLD
                     TDRFFENIAKLDKANVDAVTLADNSLATVRISNIAAASLIKQYYNIEPLVHITCRDRN
                     LIGLQSHLLGLSLIGVNEILAITGDPSKVGHLPGATNVYDVNSKGLTELALRFNQGIN
                     TDGDALKKRTHFNIAGAFNPNVRKLYGAVKRLEKKIESGMSYFITQPVYSKEKIIEIY
                     HATKHLNKPFFIGIMPIASYKNALFLHNEVPGIKMSDEILQQFEAVKDDKAKTRELSL
                     KLSKDLIDTVHEYFNGLYIITPFQNVEDSLELAAYSKSITAHKEAIL"
     gene            19892..22120
                     /gene="metE"
                     /locus_tag="O0293_000342"
     CDS             19892..22120
                     /gene="metE"
                     /locus_tag="O0293_000342"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCY0279252.1"
                     /translation="MTTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDL
                     HKENLLLQKYYHLDSIPVGDFSLYDHILDTSLLFNIIPERFQGRTIDDDLLFDIARGN
                     KDHVASALIKWFNTNYHYIVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPIT
                     FVKLSKGGHQTFEEKVKTLLPLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITR
                     EAYDYFEKAGVAKKLVIQTYFERAHLKFLSSLPVGGIGLDFVHDNGYNLKQIEAGDFD
                     KSKTLYAGIIDGRNVWASDIEAKKVLIDKLLAHTNELVIQPSSSLLHVPVSLDDETLD
                     TSVGEGLSFATEKLDELDALRRLFNQNDSVKYDKLKARYERFQNQSFKNLDYDFESVR
                     TSRQSPFAQRIEQQQKRLNLPDLPTTTIGSFPQSREVRKYRADWKNKRITDEAYETFL
                     KNEIARWIKIQEDIGLDVLVHGEFERNDMVEFFGEKLQGFLVTKFGWVQSYGSRAVKP
                     PIIYGDVKWTAPLTVDETVYAQSLTDKPVKGMLTGPVTILNWSFERVDLPRKVVQDQI
                     ALAINEEVLALEAAGIKVIQVDEPALREGLPLRSEYHEQYLKDAVLSFKLATSSVRDE
                     TQIHTHMCYSQFGQIIHAIHDLDADVISIETSRSHGDLIKDFEDINYDLGIGLGVYDI
                     HSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTRKEEEVKDALTVLVNAVKAKR
                     QE"
     gene            22163..22915
                     /locus_tag="O0293_000343"
     CDS             22163..22915
                     /locus_tag="O0293_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HCY0279253.1"
                     /translation="MTQYPLWQQLNTLKQATWVDLTHTFDPEIPRFSEFEKGEVSTLF
                     TVKDHGFYVQRWNIVTQYGTHIDAPIHFVENKRYLEDIDLKELVLPLIVLDFSTEVAN
                     NNDFIVTRAHIEAWEKEHGTIEPGTFVALRTDWSKRWPNIEKFENKDANGQQHAPGWG
                     LDALKYLIEERRVEAVGHETFDTDASVDVVKNGDLVGERYILGQDKYQVELLTNLDQL
                     PTRGAIIYAISPKPKDAPGFPVRAFAIKPSND"
     gene            complement(22998..24179)
                     /locus_tag="O0293_000344"
     CDS             complement(22998..24179)
                     /locus_tag="O0293_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCY0279254.1"
                     /translation="MTRVVLAAAYRTPIGVFGGAFKDVPAYDLGATLIEHIIKETGLN
                     PSEIDEVIIGNVLQAGQGQNPARIAAMKGGLPETVPAFTVNKVCGSGLKSIQLAYQSI
                     VTGENDIVLAGGMENMSQSPMLVNNSRFGFKMGHQSMVDSMVYDGLTDVFNQYHMGIT
                     AENLVEQYGISREEQDTFAVNSQQKAVRAQQNGEFDSEIVPVSIPQRKGEPIVVTKDE
                     GVRENVSVEKLSRLRPAFKKDGTVTAGNASGINDGAAMMLVMSEDKAKELNIEPLAVL
                     DGFGSHGVDPSIMGIAPVGAVEKALKRSKKELSDIDVFELNEAFAAQSLAVDRELKLP
                     PEKVNVKGGAIALGHPIGASGARVLVTLLHQLNDEVETGLTSLCIGGGQAIAAVVSKY
                     K"
     gene            24718..25851
                     /locus_tag="O0293_000345"
     CDS             24718..25851
                     /locus_tag="O0293_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HCY0279255.1"
                     /translation="MVNALTPKHLAEKRAGFHELFFDLIFVYAIQKIAHVILTTQNGS
                     ISADLFFKYIVMSLFLWLMWSHQTFFTNRFGQVTFKDVSFMMFNMFIMVFLSNSLYPD
                     FEKTFFPFFLCVAIMYLSIGLQYLLHIRTGLDYGDKRTCQAFASVAFVISFLSFLSLV
                     LPQSIHYIPGFLGVFIAATGLIPFQKYLVLSPVNMMHLVERFSLLTIIIFGEVLVGLA
                     SSSFSIDHFSYIYIFQFMILISLFGVYWIITENYINHKLTSIGFRLSYTHLLINIALG
                     VINAAIVFSNNNKLNDLFEINMMYISVLIFYIGLWLITPYFHNELTNAKYISYSLGIL
                     VMSYIVSLLFKGHDHVMIISVSVATFCIMLIYFKNQRLRNKHE"
     gene            complement(26218..26847)
                     /locus_tag="O0293_000346"
     CDS             complement(26218..26847)
                     /locus_tag="O0293_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HCY0279256.1"
                     /translation="MKGMFLSSFYATRKQTYIYFIVAIIAAGYFAVFNPLMSSAMAGV
                     MLITPITDNIKHEKDSRWMYYVSTLPVKRSDYIKSYFAFYLILFGASLMIGLVVTTIV
                     TQSVMIGIMSGLMSFGIIGAYSIIFPLTFKFGAENSNVIMICASILLLISFVVFFFIY
                     GMVSGASALEFEKISTEGWLVVIAYAVIGIVITSVSYILSIKIFNKQEL"
     gene            complement(26847..27689)
                     /locus_tag="O0293_000347"
     CDS             complement(26847..27689)
                     /locus_tag="O0293_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0279257.1"
                     /translation="MTTLLNVDSVNKQYKDSDFKLQDASLTISTNETVGLIGKNGSGK
                     STLINILVGNRHKDNGSITFFGEEHTVDDVEYKEHIGVVFDDLRVPNKLTIKDIDKVF
                     QSIYMTWNSQKFFDLIKYFELPLQTKIKTFSRGMRMKIALTIALSHDVKLLILDEATA
                     GMDVSGREEVMEILEDFVAQGGGILISSHISEDIEHLADKLVFMKDGRMILTEQKDIL
                     LAQYGIVTTEDKDVEIPKHLIIASRLSKGKYQILVKDYAEIENAEPLKHIDDATKIIM
                     RGEV"
     gene            complement(27714..28427)
                     /locus_tag="O0293_000348"
     CDS             complement(27714..28427)
                     /locus_tag="O0293_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HCY0279258.1"
                     /translation="MKILRYIGYLLLGGLVGGIIGGILGNFDGFGIENLTFATYNNVV
                     VISIVATIIIILVEAIVLMNQRRALKYKQLVDKEVDIDATDQYELLANRYVLNGSILS
                     ILQTVIAFLVLLIFVVGHAAANAILFFLIPFFASAIFNTQFTLFNRRFDDRIPKIADK
                     NYTEKRLEILDEGERHIELIALFKTYAINLSILILAIVVIGLYSITTGINQSFSLLLI
                     IAIFIYNAFSYLLKRRRFY"
     gene            complement(28424..28627)
                     /locus_tag="O0293_000349"
     CDS             complement(28424..28627)
                     /locus_tag="O0293_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0279259.1"
                     /translation="MRNRLKELRARDGLNQTQLAKQAGVSRQTISLIERNNFMPSVLT
                     AIKIARIFNETVETVFIIEEDEA"
     gene            28868..29257
                     /locus_tag="O0293_000350"
     CDS             28868..29257
                     /locus_tag="O0293_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HCY0279260.1"
                     /translation="MAFTLSAIQQAHQQFTGVDFPKLFKAFKDMGMTYNIVNIQDGTA
                     TYVHQSEDDIVTSSVKSNHPVAQKSNKTIVQDVLTRHQQGQTDFETFCDEMAEAGIYK
                     WHIDIQAGTCTYIDLQDQAVISELIPQ"
     gene            29357..29581
                     /locus_tag="O0293_000351"
     CDS             29357..29581
                     /locus_tag="O0293_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HCY0279261.1"
                     /translation="MIIMITNTFILGITGPTSLVVISIIALIIFGPKKLPQFGRAIGS
                     TLKEFKSATEDLDKESHDTPSKESKQQREQ"
     gene            29598..30299
                     /gene="tatC"
                     /locus_tag="O0293_000352"
     CDS             29598..30299
                     /gene="tatC"
                     /locus_tag="O0293_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HCY0279262.1"
                     /translation="MVHFSELRHRLVKILLSFVITVIVVYVSSFWWMTPFITYITRAH
                     VSLHAFSFTEMIQIYVMIIFFIAFCFISPVMFYQLWAFIAPGLHNNERQFIYKYSFFS
                     VLLFCAGVAFAFYVGFPMIIQFALKLSLTLNISPVIGFKAYLIELIRWLFTFGLLFQL
                     PILFMGLAKFGLIDTTSLKHYRKYIYFACFVLASIIAPPDLTLNILLTLPLILLFEFS
                     MFIVKFTCRGKPPTH"
     gene            complement(30391..32103)
                     /locus_tag="O0293_000353"
     CDS             complement(30391..32103)
                     /locus_tag="O0293_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCY0279263.1"
                     /translation="MKHYLTKFVAMLITAAMVCSFGLLKSQAAEQQSISDVYSVITDA
                     KSALSNNSISNDNKQKAIEQVVSAVKKLSLEDNSESNAVKSDVRKLEDAKANDNQKDT
                     LSQLTKSLIAYEEKLASKDAGSKIKLLQQQVDAKDAAMTKAIKDKNKAELESLNNSLN
                     QIWTSNETVIRNYDANQYGQIEVALLQLRIAIHKSPLDTAKVSHAWTTFKSNIDHVDK
                     KSNTSANDQYHVSQLNDALEKAIKAIDDNQLSDADAALTHFIETWPYVEGQIQTKDGA
                     LYTKIEDKIPYYQSVLDEHNKAHVKDGLVDLNNQIKEVVGHSYSFVDVMIIFLREGLE
                     VLLIVMTLTTMTRNVKDKKGTASVIGGAIAGLVLSIILAITFVETLGNSGILRESMEA
                     GLGIVAVILMFIVGVWMHKRSNAKRWNDMIKNMYANAISNGNLVLLATIGLISVLREG
                     VEVIIFYMGMIGELATKDFIIGIALAIVILIIFALLFRFIVKLIPIFYIFRVLSIFIF
                     IMGFKMLGVSIQKLQLLGAMPRHVIEGFPTINWLGFYPSYEPLIAQGAYIMVVAILIF
                     KFKK"
     gene            complement(32084..33313)
                     /gene="efeB"
                     /locus_tag="O0293_000354"
     CDS             complement(32084..33313)
                     /gene="efeB"
                     /locus_tag="O0293_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCY0279264.1"
                     /translation="MTNYEQVNDSTQFSRRTFLKMLGIGGAGVAIGASGVGSMWSFKS
                     MFNTPEDPEKDAYEFYGKVQPGITTPTQKTCNFVALDLKSKDRDAIKAMFKKWTVMAD
                     RMMDGDTVGKPSNNPLMPPVDTGESIGLGASKLTITFGISKSLMKKIGLSSKIPDAFK
                     DLPHFPNDQLIDDYSDGDIMIQACSNDSQVSFHAVHNLVRPFRDIVKVRWAQSGFISA
                     KGKETPRNLMAFKDGTINPRKNNQLKDYVFIDDGWAKHGTYCVVRRIQIHIETWDRTA
                     LEEQEATFGRKRHSGAPLTGGKEFDEIDLKAKDSHGEYIIDKDAHTRLAKEANTSILR
                     RAFNYVDGTDDRTGNFETGLLFIAFQKATKQFIDIQNNLGSNDKLNEYITHRGSASFL
                     VLPGVSKGGYLGETLFD"
     gene            complement(33310..34164)
                     /locus_tag="O0293_000355"
     CDS             complement(33310..34164)
                     /locus_tag="O0293_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279265.1"
                     /translation="MKKLTTLLLASTLLIAACGNDDSKKDDSKTSKKDDGVKAELKQA
                     TKAYDKYTDEQLNEFLKGTEKFVKAIENNDMAQAKALYPKVRMYYERSEPVAEAFGDL
                     DPKIDARLADMKEEKKEKEWSGYHKIEKALYEDKKIDDVTKKDAQQLLKDAKELHAKA
                     DTLDIIPKLMLQGSVDLLNEVATSKITGEEEIYSHTDLYDFKANVEGAQKIYDLFKPI
                     LEKKDKKLSDDIQMNFDKVNQLLDKYKDNNGGYESFEKVSKKDRKAFADAVNALGEPL
                     SKMAVITE"
     gene            complement(34432..34977)
                     /locus_tag="O0293_000356"
     CDS             complement(34432..34977)
                     /locus_tag="O0293_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0279266.1"
                     /translation="MFGMKVNEQITLKILEAHDTEALFNLVNRSRNSLREWLPWVDAT
                     EQPSDTRAFIKRGLLQFADGNGFQCGIWYEGTLVGVIGLHEINHMHRKTSLGYYLDKE
                     FEGHGIMTQAVEALIKYCFEELDLNRIEISAAVNNEKSRAIPERLGFTREGMLRDNEL
                     LNGIYSSSYIYSLLKSEYDQK"
BASE COUNT        11505 a         6515 c         5148 g        11906 t
ORIGIN      
        1 taaatcatca actacaaaat ccattatcaa agcactttat attatctttc tttattttaa
       61 aagtaatcat tcactttaca aagttatcca gttgactatt ctggatcaaa atgtaataat
      121 gctaactaag agattagaat tattataatt aagcgcaatc aattgatagt tctaattcat
      181 acttaaattc ttaggaggaa gatatttatg tcattaatta acaaagaaat cttaccattt
      241 acagcgcaag ctttcgatcc aaaaaaagat caatttaaag aagttacaca agaagattta
      301 aaaggttctt ggagcgtagt atgcttctat cctgctgact tctcattcgt ttgtccaact
      361 gaattagaag acttacaaaa ccaatatgaa gaattacaaa aattaggcgt aaatgtattc
      421 tcagtatcaa ctgatactca cttcgtacac aaagcatggc atgaccattc agatgcaatt
      481 agcaaaatca cttacactat gattggtgac ccatcacaaa caatcactcg taattttgat
      541 gtattagatg aagctactgg tttagctcaa cgtggtacat tcattatcga cccagacggt
      601 gttgtacaag catctgaaat taacgctgac ggaattggcc gtgacgctag tacattagct
      661 cacaaaatca aagcagctca atatgttcgt aaaaaccctg gcgaagtatg cccagctaaa
      721 tgggaagaag gcgctaaaac attgcaacct ggtttagatt tagtaggtaa aatctaagga
      781 ggcattacaa ccatgcttaa tgctgattta aaacaacaac ttaaacaact attagaacta
      841 atggagggca acgttgaatt cgttgccagc cttggttcag atgataaatc caaagaactt
      901 aaagatttgt tgacagaaat tactgatatg tcacctagac tatctctttc tgaaaaatct
      961 ttaaaacgta caccaagttt ctcagtcaat cgtcctggcg aagaaacagg tgtaacattt
     1021 gcaggtattc cattaggtca cgagtttaac tcacttgttt tagcaatttt acaggttagt
     1081 ggtcgtgcac ctaaagaaaa acagtcaatc attgaccaaa ttaaaaaatt agaaggttca
     1141 ttccattttg aaacattcat tagtttaacg tgtcaaaaat gtcctgatgt cgttcaagca
     1201 cttaacttaa tgagtgtgat caaccctaac atcacgcatt ctatgattga tggtgcagtg
     1261 ttccgtgaag aatctgaaaa catcatggca gtccctgctg tctttttaaa tggcgaagaa
     1321 tttggcaatg gtcgtatgac aatccaagat attctttcga aactaggcag tacggcagat
     1381 gcatctgagt ttgaaaataa agaaccttat gatgtcttaa tcgttggtgg tggtcctgct
     1441 agtggtagtg cagcgattta cacagcacgt aaaggtttac gtactggtat agttgctgat
     1501 cgtatcggtg gccaagttaa tgatactgct ggtattgaga acttcattac tgttaaagaa
     1561 acaactggtt ctgaattttc ttctaactta gcagcgcaca ttgatcaata tgacattgat
     1621 gcaatgacag gtatacgtgc tacagatatc gaaaagactg acgaagcaat taaagttacg
     1681 ttagaaaacg gtgctgtctt agaaagtaaa acagtcatta ttgctactgg tgcaggttgg
     1741 cgtaagctaa acattccagg tgaagagcaa ttgattaata aaggtgttgc attctgccct
     1801 cactgtgacg gacctctatt tgaaaataaa gacgtagcag ttatcggtgg cggtaactct
     1861 ggggttgaag cagcaattga ccttgctggt atcgttaatc atgttacatt attcgaattc
     1921 gctagcgaat taaaagcaga caacgtgtta caagatcgtt tacgttcttt atcaaatgtt
     1981 gatatcaaaa caaatgccaa aactactgaa gttgtcggag aagaccatgt tacaggtata
     2041 cgttacgaag acatgaacac cggcgaagaa catctactta acttagatgg tatctttgtt
     2101 caaattggtt tacttccaaa cacatcatgg ttaaacgatg ctgttgaatt aaacgaacgt
     2161 ggtgaaattg tgattgatcg taacaataat acgaatgttc ctggaatatt tgctgctggc
     2221 gatgtcacag atcagaagaa caaacaaatt atcatttcaa tgggcgctgg tgcaaatgca
     2281 gcattaaatg cctttgacta tattatcaga aactaaagtt tcattcattt cactgacagt
     2341 atcttacatt tgttaagatg ctgttatttt tttatcccga cataaaatca tgttatgata
     2401 aacttaatat atacaacgag atgtgcgata atgcttaaag tatgtacctc tcgcaaacaa
     2461 atcaatatga aaaatgatat tgagaaagaa aagtatgtga acgaaattat tatgctttta
     2521 cgtacgatac ctacgtctaa attagtacaa ccaattatgc agttatttat agataaagct
     2581 atccttaaca acgtccaaaa tgatgcatta ttgatagaaa aagcaactca tgcacgtaat
     2641 catttacgtc tatggtcatt gcaaggtcat cgtgacattc caaactaatt caatgttact
     2701 ctattaaaca tgaatacata ttttttcaat taaagataaa ctaaggagtt atattacatt
     2761 atgaaaaaga gattactact aagtacattt ttagcatcga cattaattct tacaggttgc
     2821 gcgtccgatc aatctgataa cgaagatcat catacaagta ctggcatcca tgcacctaaa
     2881 agcgctaaaa aattagaaac aaaagatatc tttaactcag ataagaaaaa tagtgatatt
     2941 agtgatgcag agatgaagca agctatagaa aagtatttgt ctgtaaatag cgatatactt
     3001 gataacaaat atattatgca acataaacta gataaacaaa ttgacagtca aacaaaagtg
     3061 actgaaaaac aagcagaaac acttagccac ttatctaact tagctgttaa aaacgatttg
     3121 catttcaaga aatttgtcac tgaaaacaat attcctaaag aatataagaa acccgttgaa
     3181 ttaatgatga attacttcaa agctttaaat agtacaatcg ccaatgttga tgaagatatt
     3241 gaaaagctta gctatcaacc gcaaaataaa atcaatgttg tcgacgtgcc aacaaaatat
     3301 gccggtgatg ttaataaaaa gcaacaagat aaaattaaag atttcttaaa atctaaaggt
     3361 atcaagagtg atgttatcga taaataactg aaagccatca ttgcgaaatg taacacatca
     3421 ttacatgagt tttaattcat gacaattctt tatctttaaa agtgactaat caatttagct
     3481 aaagtcatca tttgggcatc aagcagcata ttactgtttg atgccttttt aattgcctta
     3541 aattctctat ctatgccaaa attcccatta gttgacgcat ttgactatat acctaacata
     3601 ttccatactt atattgactc tataaacaaa ggatgatcat gatgaaagtt cgaattactt
     3661 ttattatttt agcaatttta tcgacgattg tttgcttgtt cttagcagca atgcatccaa
     3721 caggaccaaa cacagtaact tttgaacagc catatttatt caccctcaac atcattataa
     3781 tggtattagt cgcattacct tcacttgttt tagctatata tgattatatg agttttagaa
     3841 tttcttctgc tattttacaa tttctagggg ctatctcttg gttcttttta tcattgatat
     3901 tatcgctcac acaatttaca ccttttacat tagcgtcatt tataacttca attattttgt
     3961 tcacaagcac aattatcaca ttagccattg gtggtaagtc tgttgaaaag aatgattccc
     4021 cttaaattcc aaatgaaaaa aaggttctga aggccgctat aaaacacagt ttttcagaac
     4081 ctctatactt ctattcaatg atatatggtt tgcaattttc tacctttaaa tccacagctt
     4141 ctgcccttga aactttgtta aaataaacca tcaaacaacg aatgacaact tgatgtgcaa
     4201 caatgacaat atcatctttt tgtgtatctt cattgacaac atgattcata aaatgttcta
     4261 cgcgttgata tacatcttca taactttctc cttcaggcgc tttttgtgaa aaactatgac
     4321 gaaagtcttt aaagtttgga tcattgaaat atttatcata tttcggattc gcactgattt
     4381 catctttata ttcaccctca aatacgccaa gtgaacgttc tcttaataga ggggtagtcg
     4441 ttgatgcaat gtcatatgga aaaatatgtt caaacgtttg ctgtgttctt aataagtctg
     4501 aaacatatac atgtttaatc tgtttctctt taaaataatc acataaatcg tctgcacttt
     4561 ttgtgccagt atccgttaac ggcacatcta attgtccaca aaaataagat cgaaaatgtt
     4621 tattatcata attcgatttt gattcgccat gtctaactaa ataaatcgtc ataatattac
     4681 tccttacctt atgtatttca tatctaccat aacactttga ctactaattc gatatcaatc
     4741 ttaatattct attctaaaaa aagaattaat tcatatttta aattaatgac tctaaaaata
     4801 gtattgtctt taatcaagtg atgtgttact atatgggtgt agttcttaaa tgactacaca
     4861 gtcttttata ttcatgaaca taatactcct ggatggcgca acccaacgtc atcctttttt
     4921 tatttgtcaa atactcgctt ttttatttcc aaaatttcct catattaatt tattactttt
     4981 taatcctatc ttatctacat tttcaaatat tataatacat attgaccatt ttcacgcaaa
     5041 taaaaaagat gacaactgaa tgccatcttt atttcaattt ccaattgaat ttttttatta
     5101 tttacgcata gctcttaaaa ttaacgttac gattgcaatt aagataattg aaccaattaa
     5161 tgctggcaag atgtaaatac ttcctaattc aggaccccat tgtccgaata gtttgccacc
     5221 tacccatgat ccaataatac ctgcgataat attgcctaaa ataccacctg ggatatcttt
     5281 acccataata gcaccagcag cccatccaat taagccaccg acaattaaca ttccaataaa
     5341 tccaaacata attttcagtc tcctttttct atttattttg cgttattcta agtagtaccc
     5401 cttatttaca attctaaaac aaattcaaat tatttttatc caaatatttt taaaagtagt
     5461 aattgaatat caattttatt caatgtagct atcgttattt aaagtctctg taccgataat
     5521 atcatataca tttacattat tttttctgcc gaattcatag cttgattatt ttatgttata
     5581 ggactagaat atacacatat tattagagca tctttgaatt ttaaatcaag aagcgaggtt
     5641 aatgaacaat gaatatgcat attttatata acttacgaac taaacataat ttagaaattg
     5701 acgaattagc acagcaatta aatgagaaat atggtactaa atatgaagca catcaaattt
     5761 gggaatggga gaatcatcac catgaaccta aatttaaaga tgccatgcat ttagctgact
     5821 tctttgatgc accatatgaa atgtttttag aaagtaaggt taaagaatat cagaaacatt
     5881 tagaagaagt cgatattcgc atggataaat agatgcaaat aaaccctcac aacacgtttg
     5941 gcatatatcc tttcaaatct atactggata tattacatta cgttgtgagg gtattttatt
     6001 aattaatatg aattaagaca ttttacaagc gttaatgcaa cgaatctttt tagtgatctt
     6061 gctcactctt taatacttta ccgttcttag catcaacagt aacttcttgt tttttattac
     6121 cttttttcaa atcgatattg taaacaagtt tgccatcatc tttttcaagt gaccattctt
     6181 taatatcacc atcaaattct ttttgtcctt ctttaatggc ttttttgtaa tctatagcat
     6241 cgctatattt aaagttatca ttttcattca ttgtatcttc tttttcagtc tttttgttaa
     6301 tcactttttt atttttatca gcaacaagta cttctgactc ttcaccagat ttttgttgcg
     6361 tcactttata agcccattca ccattagaat tttcaaatga aattcctttc aacttttggc
     6421 ctttgtaagt ttcttcagct tttttcacag catcttctgg gcttgtttta acatctttta
     6481 aagcaataac atcttttgtt gtattagtgt cttgattagt atttgactct gttgattttg
     6541 tttcatcttt tggagtatca ttgccacatg cagtaagcac caccgctgac attgataaca
     6601 ctgctaatga ttttaatttc ataatatcac tctctcttcc tatttttgaa actcataaca
     6661 aaagcttata tgctatatag attgtattac cccttgtttt taattttatt cataattatt
     6721 acaaatattt ttaaattaat cgtcatgcgt tactttcgtt cgtactcctt ttataaatga
     6781 accatgtaat ataagcatgc tattatcgac tcagtttgtc taatgctttc tttggtactt
     6841 cttccttttc aacttcttca aaagtttcta catgatgacc tttatgtgta attttcaagt
     6901 atctatgcgg tttaacatca aatgtagcag tatacattaa ttgcgtctct ttcccttctt
     6961 tattaaaaac actttgacta taacttcgga gttgatcatc cattctagta gacacctctg
     7021 tcgttttaac ataagaatca tccttcttta ctaatgggtt aaattgatct gttataccat
     7081 gggagtctat tgtttttaaa atgaataaag cagcataagc gccaatgata gtaagtacaa
     7141 gatatgttat tgtttttaaa atcgttttca aaagatcacc tctgacaaaa tataaaatag
     7201 gatgtatctg aaaacaactt acataattgt aattaaagtg taagtattag gagaaaatga
     7261 tggtatttga atctgaaaga tacgaatcgt ttttaaacta agttatttta tagagacttt
     7321 attatgaaat tgaaagaagt taattttaag gttgtcgaat gtaaaaacat attgattaac
     7381 cataaaagag aaattatttt tgtagatttc ggcataaacg tcattaaaga attttctata
     7441 tgatggtgct aatcataaca aagaaagcta ggcaccttta gatacctaac tttcaaagta
     7501 ttagcgataa aacactatat atactaattt agcttaattt caaacttgtt caatgtcatt
     7561 aacacttttc acaagtttga aagttacatt ttttatatcg gctgtattaa tttttacatt
     7621 ttccctatct ttttgtagct ctttatgcga ctctaatgta tatttaccac catcttttgt
     7681 atttatgacg atgttacctt taggcaaatg ttctcccata tacaagccat aggaaatatg
     7741 agcaaatctt atgatatgat ccaattcttt taaagtgaca acttctttat taagagtaat
     7801 attcctctta ggtgtataca ctctgccagc aactgtgtat gttccttcta aatgtatacg
     7861 aacttggttt tcatttaaag gtctctctga ataaacccaa ttcctagatt tattagattt
     7921 tccatttatt gtatccttgc tgtatctatc gtaaagtttt ttaaccaaag ctttttctga
     7981 attatttggt acttcttcaa ctttttgtaa ttgtttatct tgtacacttg aggaattttg
     8041 tgttgacgcg tctgcttttg gatatattcc aaatgttccc ccatagatga tacctagcga
     8101 tagaatagat tttaatacga ttgaattttt tgagtcatat tttttgaaca tatttaatta
     8161 cctccttgat gtaaagcttt atttgctaca attataaaaa taatagacgt gttcatgaat
     8221 taaattcatc ttaactcttg attaacttta atttgctacc actctgaatt taataactat
     8281 aaatcgtcta cacataattg gacaaaatct aagagaataa aatttgttaa tttaaaatag
     8341 caagcaattc aaagttatat gtgtaataga taaaatagat atccctatag tgatgcgtta
     8401 ctagctaaac ataataacac attagaagat aatgaagtta aggagttact ggattgtttc
     8461 gactatgtaa ttaagtataa aaatatccaa cgacaaaacg taattataaa atggtaaaag
     8521 ctatggtaca gtttcaaatt gctaatgaca tgcgtatcgg tgagctactt gcaataaaga
     8581 gagtaaatat aaactatgaa gataaaacgc tagatatcga cggtaaagtt aattggataa
     8641 ctgaaaaaag acgggagcat tcggagtaaa ggagacaact gaaagaagta atagctataa
     8701 ggccacaggg ctcactaccc aaagcatcga cttactaaga acacttatgc ttggaatgat
     8761 aagtttattg atagagagta catattcaca aatacgtctg gtagccctat cgactcgaac
     8821 aaaattagcc acattattaa aggggggcgc tgatattagt tctattaaga aacctataac
     8881 gacgcataca ttacatcatt cgcatatatc tacacttgct caattaggaa ttaacttaaa
     8941 agcaatgcaa gagcatgtag gtcattcaga ttataaaaaa aatctagaga tatacacaca
     9001 tgttactaat cagatggcga aagatatgat gaataaattt gaacgattgg ggagttaaaa
     9061 ttggaaaaag atgatacact agcagaaatt aagcctatgc tcaattttga tgagcaaata
     9121 gcgaaattaa aacagatgaa tatatttttt aatattattg acaccgaaaa agcaaatgaa
     9181 attcttagaa aaaataatta cttcttcaaa ctagcttatt tccgaaaaaa tttcgaaaaa
     9241 aagaatggcg gctatttcat agaatttgct tatttatcag atttagcaac tatagatatg
     9301 aaattaagat acacaatgtt gcatttaact ttagatattg aacatagttt aaagtatcta
     9361 gtcttaaaac taataacaga aaataaccaa gaagatggtt ataaaataat agatgagttc
     9421 ttatgtattg ataaatcata tagcaattca aattttgaca caaattcaag aacaccagaa
     9481 gaagttatgg aaaccaaaat caaaaataaa aacgaaatat tcaagcatat gaataaacga
     9541 ggacaactac ccgagaagtt gaataaatac tatcaaaatc cacccgcatg ggtttgcatt
     9601 gaattcatgc aactaggtca attcgtttcg tttctcaact tctattacaa gaagtacaat
     9661 gacgaagaat tgagagttgc taatatttta atgcctttag ttaaaaatat aagaaacaaa
     9721 tcagctcata accaacccat catagcaaat ctaaattatg acagtagatt acctcaatat
     9781 ttatttgaaa aagggaataa tataggcata tctagaaaca tgttcggaat aaaaaatttc
     9841 atagatactt tcgctacgct agaattacat aatcaagttt gtagtaatgc aattatccaa
     9901 gcaagatatc acgatttgga ccaacttcaa aagcgatata aaagaaacga aagctattat
     9961 aataatgcat tagctatcaa aagatttttt atagctttag ataaaattat tgacttcaac
    10021 agaccaaaag tataaactat ctagtgagga aagagactta taggtctcgc gagttatttt
    10081 aattcgtatg caagaaaaag aagagctatg cattttattt aaaatgcgta gttctttttt
    10141 tatgcatcta aattcatatt atttttgcaa tataaacata tctttgtgca aattccgaac
    10201 acaaaacatt cacatcatcc ttttttgccc tttttctata ccccaaaaca caaaaagccc
    10261 cgtaagccta tgcctacggg gtttgacaat aaattatata ttattgttct tctttaacat
    10321 atggtaataa tgccatatga cgagaacgtt tgatagctgt agtcaacata cgttgatatt
    10381 tagctgaagt accagttaca cgacgtggta aaattttacc gcgttctgag ataaaacgtt
    10441 ttaataattc agtgtctttg tagtcgatat gtgtaatacc atttgctgtg aaatagcata
    10501 cttttttacg acgacgtccg cctcttcttg gtccacctgc catgattgtg tgcctccttt
    10561 gataattttt tcgctttaat ttcgttaatt tttattagaa tggtaagtca tcatcactta
    10621 tatcaatcgg tccgtttgca tttgcaaatg gattatcaga ttgtttcgtg tttgatgaat
    10681 tattgtacga attgttttgt cctgattgtt gaccaccgaa tccttgaccg taatcttgga
    10741 attcattttg ttgacgttgg ccaccatttt gttgcgcatt tttaggttca aggaattgaa
    10801 cgctatcaca cacaacttca gtaacaaaca cacgacgacc ttcttgattt tcataattac
    10861 gggattgtaa gcgaccatct acaccagcta aactaccttt agataaatag ttatttacat
    10921 tatctgcttg tcttctaaaa acaacacagt taataaaatc tgcttcgcgc tccccttgag
    10981 cattcgtgaa cgtacgattt actgcaagag tgaatgtcgc tacactcaca cctgagggag
    11041 tggttctgta ttccggatct ttcgttaaac gacctactaa tacaactcta tttagcattt
    11101 aaacgccccc tctaattatt acttgtcttc gtcttcacga ataaccatgt aacgaatgat
    11161 atcgtcactg attttagcta gacgttggaa ttcgtcagta gctttgttgt tatcagattt
    11221 aacacgtacg atgttgtaga agccatcttt gaaatcattg atttcataag ctaggcgacg
    11281 tttaccccag tcttttgctt ctaaaacttc tgcaccttca gtagctaaga taccgttgaa
    11341 acgttcaact aacgcttttt tagcatcttc ctcaatgttt gggcgtacga tgtacataac
    11401 ttcatatgtt ctcattttat atttgcacct ccttgtggtc tatacggctt atcaatctta
    11461 aaacagataa gcaaggaata attttcatta ctcacaataa agaattatat catgcgccat
    11521 tactttttac aataataatt caaactactc ttcatatcat ttttgatatt aattcatttg
    11581 aaactttcta tgatattttt aaaaatacac ttcacaaaag cgaacatatg ttctataata
    11641 gttgtgaggt ggtaaggaat gaatttaaat attgattggt ctaaagattt tcaagaattc
    11701 caagagatac ttaatagtgg tattcatcct gaatggcttt attgtgcaaa ggctaatctt
    11761 gttttagagc ctgcttatac tggcgaaggc aaacaatttt tcagcactca agatattatt
    11821 aacgcgagta aaattattcc attcttttaa taaattatac gcataattaa aagaggcgaa
    11881 caattgattc cgcctcttct acatatatac tatttaatta acttcactct atcgtttata
    11941 cattaaatct gaaatgaacg atatcgccat cttgcataat atattcttta ccttctaatc
    12001 gttgtctgcc cgcttcttta gcgccacttt cgccaccata ttgtacatag tcgtcataac
    12061 ttgttacttc ggcacggata aatccacgtt caaaatcagt atgaatgata ccagcacatt
    12121 gaggtgcagt cataccttgt ttaaatgtcc aagcacgtac ttcttgcaca ccagcagtaa
    12181 aatatgttga taatcctaat aattcataag ttgttctaat taatcgatct aatcctggtt
    12241 cttcgatacc taaatcttct aagaacattt ctttatcttc atcatctaat gtagcaattt
    12301 cttcttcaat ttttgcacta ataacaatca cttcagagtc ttcttgcgct gcatattcac
    12361 gaatcgcttt tactttatca ttatcatcat caccaatttc atcttcacca acattagcga
    12421 tataaagcat ttttttagaa gtcagtaatt gcgcttgatt cacccatttt tgatcttctt
    12481 cattaaagtc aatactacga gcgggtttac cattttctaa agcttcttta atagttgtta
    12541 aaatacgtac ttccatttca gcagtcttat ctttttgacg tgctaatttt tcaattctag
    12601 gcaaacgttt ctcaacagat tctaagtccg ctagtactaa ttccatatta ataacttcaa
    12661 tatcatcaat agggtctact cgaccagcaa catgagttac gttatcatca tcaaatgcac
    12721 gaacgacctg acaaatcgca tctacttctc taatatgtga taagaattta ttacctaacc
    12781 cttctccctt tgaagcacct ttcacaatac cagcgatatc tgtaaattca aatgtagtcg
    12841 gcaatgtctt tttaggttga accatttctt ctaatttaag taatctagca tctggcactt
    12901 ctactatccc tacattagga tcaatcgtag cgaatggata gttcgctgct aaagcacctg
    12961 cttttgttat tgcattaaat aatgttgatt taccaacgtt tggcaatcca acgatacctg
    13021 ctgttaaagc cattaatcat tctcctatct ttgtgtatca tcatgagatt cgatgatttt
    13081 tttaagtttt ttatcaaacg tttgacgtgg aatcataata cttctttgac aattttcaca
    13141 tttaattctt atgtctgcac ccattctaat aatcttaaaa cggtttgttc cacacgcatg
    13201 ttgttttttc atttctacta tatcatttat tccatatttt gacgccatta aatatgacct
    13261 ccatgtatta taaactacta aacaccgttt tcactatgat tataagcagt cataattggt
    13321 tgaggtgttt taataccttc ttgtaaaaac attttttgta cttctttgcg aataattcga
    13381 gctccagcaa atccttcacc tggaattgtt tctgcagata ttctcaatat tactcttgta
    13441 tcttcaatag catcaatacc aataacaacc ggatcactaa caaataagta atatttacta
    13501 cgtaaagaag taaatagttt gtttagtttt ttatcaacat tatcaatgtt ttcttcgaca
    13561 gacactggaa ttttaacgat agctgtacca tttgtaattg agtagttcgt tatttcaccc
    13621 atactactat ttggtaaaat tgttaattct cctgaaattg tattgattcg tgttgaacga
    13681 agtccaattg atttaactgt tccctctgct acagttgtac caccgttatt tatcttaaca
    13741 taatcaccta catcaaattg gctttcaaat ataataaaga atcctgtaat tacgtcttta
    13801 actatagttt gagcaccaaa acctactgct aagcctacga caccagcact ggcaattacc
    13861 ccttcaacac taatgccaaa tttacttaaa atcgtcgtaa taactataaa ccaaacgata
    13921 tacttcacta cattttgaac aagagatatt aaagttttag agcgcttttt gttactcttt
    13981 ttacttttat tttgaatctt aaatccctgt tcaatcattt tattcagtat tttaataact
    14041 atgagggcta cgataatata aataacaatc atagcgattt tagttgcaat gttttcatat
    14101 gtttctattt ttgttaatgg ctcaaataga gatgaaataa tattcatgac ttgattcatg
    14161 ataagcgtta ctcccttcta tatgatatat caatattgta ttcatatttt tatatacttc
    14221 caacctacga aagtctcaat atatctagaa tcaaatgttc actgattaca atttatttac
    14281 acttgcacaa agagttatta tataaaattg tgtaactatt taccatacct acgatttaat
    14341 tgttcaatta ttctcacaaa atcttcttgt gaatcaaact caaatgagat tttaccaacc
    14401 gattttttta ttgatatatc tactttggta ccatactgtt ctcgcaactg tcgttcttgc
    14461 tgctttataa atttaggctt agttatatct actttgtctg tttccgactt tgacgaaaca
    14521 ttttttaatt cattaacatg gttttctaaa tatctgacac tccacttttc tttaacaacc
    14581 cgtttcgcta acctaagcat ttgttgttca tctttaattg ccaataacgt tcgtccatgt
    14641 gcacttgtca gtcgcccatc ttttaccatg tcagcaatct ttttcggcaa atgtaataac
    14701 ctcaacatat tcgctatata cgggcgcgac ttactcaatc gtttcgctac ttcttgttgt
    14761 gtaattttca aatctgtcat caaacgttga taactttcag cttcttcaat cgcatttaag
    14821 tcttctcgtt gtaaattttc gatgaccgcc agttccatca tatcttcatc tgttaaatct
    14881 ttgataatcg ctgatacgta ttttagacca gcaattttcg aagctctaaa ccttctttca
    14941 ccaactacaa tgtaataacc ttgaactgtt tttctaagca caattggttg caaaattcca
    15001 tattgcttaa ttgaatctgc caaatcattt aaatgatttt catcgaaagt ttttcggggc
    15061 tgatacggat tcggtttgat atccgatata tctatttgct taatttgttc atcttttgtt
    15121 ttagtaatac gttgatcttc actttttgac aattcactca catcactttt tatttgataa
    15181 caacagttaa atatatttta tgtattctaa atgagtcaga caaccattaa ccacacttta
    15241 tttaaaaaat aattcatttt tcaacttcaa gataccaatt acaatttcac aaacaaatta
    15301 acttttaaat caaaattttc tgaataattg ttgacaaatc tttttttact ctgtaagcta
    15361 attaatatct tataacagtt taatgaaacg taaacacaat aaagaggaaa gtaaaacaca
    15421 ccctgcttat acagagagtc tttagtagct gagagaagat tttgaaagcg tgtttgaaaa
    15481 tggccttgga gtgttgatgc caatatgagg tgtctacggg ttcgcccgtt atagcgatac
    15541 agtattaaca ttgatgttaa atggcgtact ggattcttta cgcacgattt tttgttaata
    15601 agtatgggat agcacattac tatatcctta cttactgact ttaattgtga taattgttca
    15661 gtaagcatat ttacttttaa tgcgtactga ataaggttat ttcagcgatg gaataacaaa
    15721 taaaggtggt accgcgaaac ataagctttc gtccttttta tccgattcat tcgggtacga
    15781 aggacggaag ctttttttat tttttcttat aggagggtct taatatgaag gatacacagt
    15841 tagcccaaat cacattaacc gatgattcaa ccggtgctat agcgaatcca atccatttat
    15901 ctactgccta caagcatcca aaactaggac aatcgacagg ttttgattat acacgtacta
    15961 aaaatcctac acgctcaaca tttgaaacct gttttgccaa acttgagcat ggtattgcat
    16021 cattcgctac atcaagtgga atgtcagcca ttcaattaat atgtaatcta tttaaacctc
    16081 atgatgaaat tttagtttca ttcgatttat atggtggcac atttagatta tttgaatttt
    16141 acgagcaaca atacgatatc aaatttaagt acgttgattt tacagattat gaacaagttg
    16201 aaaaagaaat cactgataaa acagttgcat tattcattga accaatatct aacccacaaa
    16261 tgattgctat tgatgtaaag ccatactatc aactttgtaa agctaaaggc ttattgtcaa
    16321 ttatcgacaa tactttttta acaccttatc tttcaacacc actagcagaa ggtgctgata
    16381 tagtcttaca ttcagccacg aaatatattg gcggacataa cgatgtacta gcaggtgtcg
    16441 taaccgtcaa agatgaatca ctcgcgcaac agttgtttga ttttcacaac atgactggcg
    16501 caacactttc accaatagat agttatttgt tgttacgtgg acttaaaact ttgcatttac
    16561 gcattgagcg tgcgcaatca aacgctagaa aacttgctaa aaaatgtcag tcacttcaag
    16621 caattgacga agtactatat agcgggcaaa ctggcatgct tagtttaaga cttaacaagg
    16681 cctatagcgt cgctaaatta ttagaaaatt tagacatttg catttttgca gaaagtttag
    16741 gaggtactga aacattagtg accttccctt acacccaaac acatgttgat atgccagatg
    16801 ctgaaaaaga taaacgtggc attgatgagt atttaatccg cttatcacta ggtgttgaaa
    16861 attatgaaga catcgaacgc gatatcatcc aagcattaga taaagctcag attggagaga
    16921 ttgtatgaca ctttcaaaag agacagaagt gatattcgat tggcgtagag gtgtggaata
    16981 tcattcagct aacccaccac tctatgattc ttcaacattc catcaaacaa gtcttggtgg
    17041 cgatgttaaa tatgattatg cacgaagtgg caaccctaac cgtgaacttt tagaagagaa
    17101 gttagcacga ttagaacagg gaaaattcgc ttttgctttt gcatcaggta ttgctgctat
    17161 ttcagcagta cttttgactt tcaaatctgg tgatcatgtc atcttacccg atgatgtata
    17221 tggcggtact tttcgcctca ctgagcaaat tttgaatcga tttaatattg aatttacaac
    17281 cgtagatact acaaagctcg aacaaatcga gggtgccatt caatcaaaca caaaattaat
    17341 ttatatcgaa acaccatcga atccctgttt taaaattact gatatcaaag ctgtttctaa
    17401 aatagccgaa aagcatgaac tactggtagc tgtggacaat acatttatga caccgttagg
    17461 tcaatcacct ttattacttg gcgctgatat tgtcattcat agtgctacaa aatttttaag
    17521 tggacatagc gatttaattg ctggtgctgt cataactaat aatgaggcaa ttagtgacgc
    17581 tctttattta atacaaaatg gtacaggcaa tatgttatct gctcaggata gctggacact
    17641 tgctaaacat ttaaaaactt ttccaatcag atttaaacaa tctgtcgaaa acgctcaaaa
    17701 aatcgtgtca tttttaataa agcaagatga aatttcagaa gtttattatc cgggactcac
    17761 tactgctcat ttagaacaag ctaaaaatgg cggtgccgtt attggctttc gtttagctga
    17821 tgagtctaaa gcacaacaat ttgtcgatgc actgacatta ccactcgttt cagtgagtct
    17881 gggcggtgtt gaaacgatcc tttcacatcc agcaacaatg tctcacgctg cactacctga
    17941 agaagtgaga caagaacgtg gtatcacttt cggtctattc cgattaagtg ttggtctcga
    18001 agatcctgat gaactcattg cagacatcaa atacgcatta aaggaggcat tcaatgagtc
    18061 aattcctcac acaattgaaa gataatgttt tagtagctga tggcgctata ggaaccattt
    18121 tatactctga aggattagac acctgtccag aagcatataa tcttagccat ccagataaag
    18181 ttgaacgcat ccatcgttca tatattgaag ccggtgctga tgtcattcaa accaatactt
    18241 atggtgcaaa ttttgaaaag ttaaaacgat tcggtcttga agataaagtt aaagcaatac
    18301 atcaagccgc cgttcgcatc gcaaaaaaag cagcaaataa agatacgtat atattaggca
    18361 cagttggtgg gtttagaggt atcaaacaag aggatatcag cttacaaact attctttatc
    18421 atactgaaat tcaaatagac accttaattg aagaaggcgt tgacgcgcta cttttcgaaa
    18481 cgtattacga cctagaagag ttaacaaatg tcatttcacg aacgagaaag aaatacgaca
    18541 ttccaatcat tgctcaatta accgcttcaa acacaaatta cttagttaat ggtcaggcaa
    18601 tcaatgaagg attaaaacaa ctcgttcaat gtggtgcaaa catcgtggga ctcaattgtc
    18661 atcatggccc gcaccatatg caagagtctt tcacacatat tgaattacca gagcacgcat
    18721 tcttatcttg ttatccaaat gccagcttat tagatattga aaatagtgaa tttaagtata
    18781 gtgacaatgc acaatatttc ggtcaagttg ctcaaaatct aattcgcgaa ggtgttcgtt
    18841 taattggtgg ttgctgtggt acaacgccag agcacatcaa atttattaaa gaatctattc
    18901 agacacttaa acctgttaat gacaaaaaag tgattccgat accaacgaaa gcacttttca
    18961 atccatctca aaataaagtt agacaatcat taacatctaa ggttcaagaa cgtccaaccg
    19021 ttattatcga attggataca ccgaaacatt tagacacgga tagatttttt gaaaatatcg
    19081 ctaaacttga taaagctaat gtagatgcgg taacactcgc agataattca ttggcaactg
    19141 tcagaattag caatattgct gctgctagct taattaagca atattacaat attgaaccac
    19201 tcgtacatat tacatgtcga gaccgaaact taatcggctt gcagtcccat ttacttggat
    19261 tatcgctcat tggcgttaac gaaatattag ccataactgg tgatccttca aaagttggtc
    19321 acttaccagg tgcaaccaat gtctatgatg ttaattctaa aggattaact gaactcgctc
    19381 taagatttaa tcaaggtatt aacactgacg gtgatgcgct gaagaaacgt acacacttca
    19441 acatcgctgg cgcctttaac cctaatgttc gtaaattata tggtgccgtc aaaagattag
    19501 agaaaaagat agaaagcgga atgtcttatt ttataacaca acccgtgtac agcaaagaga
    19561 aaatcattga aatttaccat gccactaagc acttgaacaa accatttttc ataggcatta
    19621 tgcctatcgc aagttacaaa aacgcactct ttttgcataa tgaagtgcca ggaatcaaga
    19681 tgtcagatga aattttacaa caatttgaag cagttaaaga tgataaagcc aaaacacgag
    19741 aactaagtct taagctttca aaggatttaa tcgatactgt tcatgaatat tttaatggtt
    19801 tatacattat cacaccgttt caaaatgtcg aagattcatt agaacttgca gcatactcaa
    19861 aatctattac tgctcacaag gaggcaatat tatgacaaca attaaaacat caaacttagg
    19921 attcccaaga ttaggtagaa aaagagaatg gaaaaaagcc atcgaaagtt attgggcaaa
    19981 gaagatttct aaagaagaat tagatcaaac attaactgat ctacataaag aaaatttatt
    20041 attacaaaag tactatcact tagatagcat cccagttggt gatttctctt tatatgacca
    20101 tatattagat acgtcattat tattcaacat catccctgaa cgtttccaag gaagaactat
    20161 tgatgatgat ttattatttg atattgctcg cggtaataaa gaccatgttg caagtgcact
    20221 tataaaatgg tttaacacta actatcatta catcgtacct gaatgggaca atgttgagcc
    20281 taaagtgagt cgcaatgtat tattagatcg ctttaaatat gcccaatctt taaacgttaa
    20341 cgctcaccct gtcattgttg gtccgattac ttttgttaaa ttatcaaaag gcggtcacca
    20401 aacttttgaa gaaaaagtta aaacgttatt accactttat aaagaagtgt ttgaatcatt
    20461 aattgatgca ggtgctgagt acattcaagt tgatgagcca atcttagtta cagacgacag
    20521 cgaaagctat gaaaatatta cacgtgaagc ttatgactat ttcgaaaaag ctggtgttgc
    20581 taaaaaatta gtcattcaaa catactttga acgtgcacat cttaaattct taagttcttt
    20641 acctgttggt ggtataggtt tagactttgt ccatgataat ggctataacc ttaaacaaat
    20701 tgaagctgga gattttgata aatcaaaaac attatacgct ggaattattg atggtcgtaa
    20761 tgtatgggca agtgacattg aagctaaaaa agtcttaatc gataaattgt tagcacacac
    20821 taatgaactt gtcattcaac catcatcttc attattacat gttccagtat ctttagatga
    20881 tgaaacatta gatacaagtg ttggcgaagg cttaagcttt gcaactgaaa aattagacga
    20941 attagatgca ttgcgtcgcc tatttaatca aaacgatagt gttaaatatg ataaattaaa
    21001 agcacgttat gagcgtttcc aaaatcaatc attcaaaaac ttagattatg atttcgaaag
    21061 cgtgcgtact tcaagacaat caccattcgc gcaacgtatt gaacaacaac aaaaacgttt
    21121 gaacttaccg gatttaccaa caacaactat tggatcattc ccacaaagcc gagaagttcg
    21181 aaaataccgt gcagattgga agaacaaacg cattacagac gaagcatatg aaacattctt
    21241 aaaaaatgaa attgctcgat ggattaaaat tcaagaagac attggcttag atgtattagt
    21301 tcacggtgaa tttgaacgta atgacatggt tgaattcttc ggagaaaaat tacaaggttt
    21361 cttagtaact aaattcggtt gggtgcaatc atatggttca cgtgccgtaa aaccaccaat
    21421 catttatggt gatgtaaaat ggacagcgcc tttaactgtt gatgaaacag tttatgcaca
    21481 aagcttaaca gataaaccag ttaaaggtat gttaactgga cctgtaacaa ttctaaactg
    21541 gtcatttgaa cgtgttgatt taccacgtaa agtcgttcaa gatcaaattg ctttagcaat
    21601 caacgaagaa gtattagcac ttgaagctgc aggaatcaaa gttatccaag ttgacgaacc
    21661 tgcattacgt gaaggcttac cattacgctc tgaatatcac gaacaatatc ttaaagatgc
    21721 tgttttatca tttaaacttg caacgtcttc agttcgtgat gaaactcaaa tccatacaca
    21781 tatgtgttat tctcaattcg gtcaaatcat tcatgctatt catgacttag atgctgatgt
    21841 tatttcaatt gaaacatctc gtagccatgg tgatttaatt aaagactttg aagatattaa
    21901 ttatgattta ggtattggtc taggtgtata tgatattcat agtccacgta ttccaacaaa
    21961 agaagaaatt actacagcaa tcaatcgttc actacaacaa atcgaccgct cattattctg
    22021 ggtaaaccct gactgtggtt taaaaacgcg aaaagaagaa gaagttaaag atgcattgac
    22081 tgtgcttgtg aatgctgtta aagctaaacg ccaagaataa tcgcagcatt tatcatataa
    22141 taaactaaaa acgggtgatt ttatgacaca atatccttta tggcaacaat taaatacttt
    22201 aaaacaagca acttgggttg atttaacgca tacatttgat ccggaaatac cacgttttag
    22261 tgagtttgaa aaaggcgaag tttcaacttt attcacggtt aaagatcatg gtttctatgt
    22321 gcaacgttgg aatattgtaa cgcaatatgg tactcacatt gatgcaccaa ttcactttgt
    22381 cgagaataaa cgttatttag aagacattga tttaaaagaa ttggtattgc cgttaattgt
    22441 attagacttt tcaacagaag tagcgaacaa caatgatttc atcgtaacac gtgcgcatat
    22501 agaagcatgg gaaaaagagc atggtactat tgaacctggc acttttgttg cacttcgtac
    22561 cgattggtca aaacgttggc ctaatattga aaagtttgaa aacaaagatg cgaacggaca
    22621 acaacatgca ccgggttggg gattagatgc tttgaagtac ttaattgaag aacgtcgtgt
    22681 tgaagctgtt ggtcatgaaa cattcgatac cgatgcatct gtggacgttg taaaaaatgg
    22741 tgatttagtt ggcgaacgtt atattcttgg tcaggataaa taccaagtcg agttattaac
    22801 taatttagat caattaccaa ctcgtggcgc catcatttac gctatcagcc ctaaaccaaa
    22861 agatgcacct gggttccctg ttagagcatt tgcaattaaa ccatcaaatg actaatatta
    22921 cccaataaat ccataaacaa ataaaaacag gttatgccaa catgtaatta atactgttgt
    22981 gataacctgt tttcttatta tttatacttt gatacaactg cagcgatagc ttgaccgcca
    23041 ccaatacaca atgatgttaa accagtttca acttcatcat tcagttgatg caataatgtc
    23101 actaatactc tagcaccaga tgcaccaata ggatgtccta atgcaatagc gccaccttta
    23161 acattcacct tttcaggagg taattttaat tcacgatcaa cagctaatga ttgtgctgca
    23221 aatgcttcat ttaattcaaa tacatcaata tcgcttaatt cttttttact acgtttcaaa
    23281 gccttttcta cagcgccaac tggtgcaata cccataatag aaggatctac accatgactt
    23341 ccaaagccat caagcactgc caatggttcg atatttaatt ctttagcttt gtcttctgac
    23401 atgactaaca tcatcgcagc accatcattg attcctgatg cattacctgc tgtaactgta
    23461 ccgtcttttt tgaaagctgg tcttaatcga cttaattttt cgactgatac attttcacgt
    23521 acaccttcat ccttagtgac tacgattggt tcacctttac gttgaggaat cgatactgga
    23581 actatttcac tatcaaattc accattttgc tgtgcacgta ctgctttttg ttgtgagttt
    23641 acagcaaatg tatcttgttc ttctcttgaa ataccatatt gctctactaa attttcagca
    23701 gtaataccca tatgatattg attaaataca tctgttaaac catcatatac catgctatca
    23761 accattgatt gatgtcccat tttaaaacca aagcgactgt tgttgacaag cattggtgat
    23821 tgagacatat tctccatacc gccagctagc acgatgtcat tttcaccagt cacaatagat
    23881 tgatatgcta attgaatcga ctttaaccca gaaccacata ctttattcac cgtaaatgca
    23941 ggtactgttt ctggcaagcc acctttcata gcagcaattc gtgctggatt ttgtccttgt
    24001 cctgcttgta gtacgttacc gatgataact tcatcaatct cacttggatt caaacccgtc
    24061 tctttaataa tatgttctat taaagtcgca cctaaatcat aggctggcac gtctttaaac
    24121 gcacctccaa aaacgccaat aggtgtcctg tatgctgctg ctaatacgac tctcgtcatg
    24181 tcatattcat ctccttatga tatatacaat tttattacta atacgtctaa tgaaccaata
    24241 cgccactaac atccaattta tgaaaccaat tgctacgtat gttacatttt cattcttgtt
    24301 atgcatttaa caaatcatat tatacaatga tgtaatttgt ttgggcaatc ggaaacgtaa
    24361 acggtttcac tttaacattt caccctctta aatatgctct atcatcctca tctatagtac
    24421 aaaattatgc tttgatttac cacaaattat cattaatcat ttcgcgtaat gactctaatt
    24481 aaattctatc tatcattgca tcgctattca cagctggttt caccatcaat ttctcatcaa
    24541 ttttttatta ccatgacgtc tccagtcaaa tattaaatcg ctgatgattg ttaattccgc
    24601 aattaccttg cagtcacctc tccttccact tcatcgccaa atctgcccct attcactaga
    24661 aaaaataaat agtatactta gtaaattaat tacacatctt aaaggagtta aaattttatg
    24721 gttaatgcat taacaccaaa acatttagct gaaaagcgtg caggattcca tgagttgttc
    24781 tttgatttaa ttttcgtcta tgccattcaa aaaattgcac atgtgatttt aacaacacaa
    24841 aacggttcca tttcagctga tttatttttc aaatacatcg tcatgagttt gttcttatgg
    24901 ttaatgtggt ctcatcaaac attttttaca aatcgatttg gtcaagttac attcaaagac
    24961 gtctcgttta tgatgtttaa tatgttcatc atggtcttcc tatctaacag cctatatccg
    25021 gactttgaaa agacattctt cccattcttc ttatgcgtgg caattatgta tttaagtatc
    25081 ggattacagt atttattaca tatacgtaca gggttagatt atggcgataa gcgtacatgt
    25141 caagcttttg cgagcgtagc ctttgtgatt tcatttttat catttctatc attagtacta
    25201 ccacaaagta tccattacat ccctggattt ttaggcgtat tcattgcagc gacaggactt
    25261 attccatttc agaaatatct tgtattatca cctgttaata tgatgcattt agtagaacgt
    25321 ttttcgttac taacaattat tatctttggc gaggtactgg ttggtttagc ttcgtcatca
    25381 tttagcattg atcattttag ttacatttat atattccaat ttatgatttt aattagtctt
    25441 ttcggtgtat attggatcat tactgaaaat tatattaatc ataaattaac atctatcggt
    25501 ttccgtttat cgtatacgca tctattaatc aatatcgctt taggtgtgat taatgctgca
    25561 attgttttta gtaataacaa taaactaaat gacttgtttg aaattaatat gatgtatatc
    25621 agtgttttaa tattctatat tggattatgg ttaatcacgc cttatttcca taatgaactg
    25681 acaaacgcga agtatatttc atattcacta ggtatactcg ttatgtctta tattgtcagc
    25741 cttttattta aaggtcacga ccatgtaatg attatctcag tatcagttgc aaccttctgc
    25801 atcatgctga tttattttaa aaatcaacgt ctacgtaata aacatgaata aatagtattt
    25861 acctcggtca aagtattgga actgaatcca aaagtgtcga acttcacatt atacttttgt
    25921 gacaaacttc agttatgcta catgatcgag gtttttaaat atttccgagg aaatcgttag
    25981 cctatacaat catcaaatga tacttataaa taacatatat cctctaatcc ccactatcta
    26041 aaaggaaata attcccacac aacataacca cctcaaaaca aataaatcaa tatagatatg
    26101 tcacctatac ttatccccca catttcattc cacaccaaaa agaacagatg acaatcgtaa
    26161 agatttatca cctgctctaa atattatttc aaatactatc atcaccaaaa tcgacaatta
    26221 tagttcttgt ttgttaaaaa ttttaataga caatatataa gaaacgctcg ttataactat
    26281 accaatgacc gcatatgcta tgacaactag ccatccttca gtgctaattt tttcaaattc
    26341 taatgcagat gcaccactaa ccataccata tataaagaaa aagaccacga aagaaataag
    26401 tagtagtata gatgcacata tcataatgac attggagttt tcagcgccaa atttaaatgt
    26461 caatgggaaa atgatagagt atgcccctat gataccaaaa ctcattaaac ctgacataat
    26521 accaatcatc acactttggg tcacaatcgt agtcacaact aatccaatca ttaaacttgc
    26581 accgaataag attaaataaa aggcaaagta tgacttaata taatcactac gtttaaccgg
    26641 taaagtagat acataataca tccatcttga gtctttttca tgtttaatat tatcagtaat
    26701 aggtgtgatt aacataaccc cagccattgc cgaactcatc aacggattaa atactgcaaa
    26761 gtatcctgca gctataatag cgactataaa ataaatatat gtttgctttc tcgttgcata
    26821 aaaactactt aggaacatac ctttcattat acttcacctc gcattatgat ttttgtagcg
    26881 tcatcaatgt gttttaaagg ctctgcattt tcaatttctg cataatcttt aactaaaatt
    26941 tgatatttcc cctttgacaa tctagaagca atgattaaat gcttaggaat ttcaacatct
    27001 ttatcttctg tcgtaacaat tccatattgt gctaaaagta tatctttctg ttcagttaaa
    27061 atcattcgtc catctttcat aaacactaat ttatcagcta aatgttctat atcttcagaa
    27121 atatgcgatg atattaagat gcctccacct tgtgcgacaa aatcttctag tatttccatt
    27181 acttcttctc ttccagaaac atccatacct gcagttgctt catctaagat taataacttc
    27241 acatcatgag aaagcgcaat cgttaaagct atcttcattc gcatccctct tgaaaaagtt
    27301 ttaattttag tttgtagtgg taactcgaaa tatttgatta aatcaaagaa tttttgacta
    27361 ttccaagtca tataaataga ttgaaatact ttatcaatat ctttaatagt caatttatta
    27421 ggcactctca aatcatcaaa cactacacct atgtgttctt tatattcgac atcatccacg
    27481 gtatgttctt ctccaaaaaa tgtgatacta ccgttatctt tatgtcgatt gcctactaga
    27541 atattaatta atgtcgattt acctgagcca tttttcccaa ttaatccaac tgtctcatta
    27601 gtagaaatcg ttaaagatgc atcttgcaat ttaaaatccg aatctttgta ttgtttgttc
    27661 acactatcta cgtttaacaa tgttgtcata tccatgctcc tctttgttta attttaataa
    27721 aaacgtcttc tcttcaataa gtaactaaag gcgttatata tgaaaatagc aatgataagt
    27781 agcaaactaa agctttgatt aattccagta gtaattgaat aaagcccaat tactacaatg
    27841 gctagtatca atattgataa gttgatcgca tatgttttaa ataatgcaat taattctata
    27901 tggcgttcac cttcatccaa tatttccaat cgcttttcag tgtaattctt atctgcaatt
    27961 tttggtattc tgtcatcaaa ccttctatta aacagtgtaa attgtgtatt gaaaatagca
    28021 ctagcaaaaa atggtattaa aaagaatagt attgcatttg ctgcagcgtg ccctaccaca
    28081 aaaataagca atactaaaaa agcaataact gtctgcagaa tacttaatat acttccattt
    28141 aaaacataac gattcgcaag caactcatat tgatctgttg catcgatatc tacctcttta
    28201 tctacaagtt gcttatactt caatgcacgt ctttgattca tcaaaacaat ggcttctacc
    28261 aatatgataa taatcgtcgc aacaatcgat attacaacga cattgttata tgtcgcaaac
    28321 gtcaagttct caataccaaa tccatcaaaa ttacctaaaa ttccacctat gataccccct
    28381 acaagtccac ccagtaaaag atatccgata tatcttagta ttttcatgcc tcatcctcct
    28441 caataataaa aacagtttcc accgtttcat tgaaaatgcg agcaattttt attgccgtta
    28501 atactgatgg cataaaattg tttcgctcaa ttagcgatat ggtttgtctt gaaacgcccg
    28561 cttgtttagc aagttgcgtt tggtttaagc catctcgtgc tcgtaattct ttcaatcgat
    28621 tacgcacatc gcatcaactc cttaattaca ctgtaaatga tatgcgtctt tttgacaact
    28681 atatttgtca aatctacacc aaaaaatatg attatccacc tatgtatgac attttgaaac
    28741 aaacacctca acgcctacaa gtcataattg tttactttcg ttacaccttc ctgcataatt
    28801 aacagcattc taattttagt atgatgcacg cattttcact aaatcaaacc attcaaagga
    28861 gactattatg gcatttacat tatctgcaat tcaacaagca catcaacaat ttactggtgt
    28921 tgactttcca aaactattca aagcttttaa agatatgggg atgacttaca atatcgtcaa
    28981 cattcaagat ggcactgcaa catacgtaca tcaatcagaa gatgatatcg ttacgtcatc
    29041 tgtaaaaagt aatcatcctg ttgctcaaaa atcaaacaaa acaatagttc aagacgtctt
    29101 aactagacat caacaagggc aaacagattt tgaaacattt tgtgatgaaa tggctgaagc
    29161 tggcatttat aaatggcata tcgatattca agcgggcact tgtacttata tcgacttgca
    29221 agaccaagct gttatttcag aattaatccc tcaataaact atatttatag caacatttta
    29281 attatttcat aaaattttat tgataatcat tatcgttcgg tataaagtaa atactatata
    29341 ctacttatga gtgaggttga ttatcatgat aactaacact tttattttag gcatcacagg
    29401 cccaacaagt cttgtcgtca ttagcattat cgctttaatt atttttggtc cgaaaaaatt
    29461 accacaattt ggccgtgcca tcggttctac tttaaaagaa tttaaatctg caacagaaga
    29521 tttagataaa gagtctcacg atacacccag taaggaatcg aaacaacagc gagagcaata
    29581 gcactgacca caccttactg gttcacttta gcgaactacg ccatcggtta gtaaaaattt
    29641 tattgtcgtt cgtcattacg gtcatcgtcg tatatgtttc atcattttgg tggatgacac
    29701 cattcataac gtatattacc cgtgcacatg tgtccttaca tgcattttca ttcacagaaa
    29761 tgatacaaat atacgtgatg attatatttt tcatcgcgtt ttgtttcatt tcaccagtca
    29821 tgttttatca attgtgggca ttcattgcac ctggattaca taataatgaa cgtcaattta
    29881 tttataaata tagttttttc agcgtgttat tattctgtgc tggcgtcgct tttgcatttt
    29941 atgttggctt tccaatgatc attcaattcg cgttaaaatt atcattaact ttaaacattt
    30001 cgcctgtcat tggctttaaa gcatatttga ttgaactcat acgttggtta ttcacatttg
    30061 gtcttctatt tcaattacca atattattta tgggtcttgc aaaattcggt cttatagata
    30121 ccacatcatt gaaacattat cgtaaatata tttatttcgc ttgctttgta ttagcaagta
    30181 tcattgcacc acccgattta acattaaata tattactcac attgccactc atattgctct
    30241 ttgagttcag tatgttcatc gttaagttca catgccgagg caaaccgcca acacattaac
    30301 cttatacgaa tcaatgctgt gattatcgtt atactgcaat accgaatatt tccaatatag
    30361 caacaaaaaa acaggcactc ggcctgtttt ttatttttta aatttaaaga ttaagatagc
    30421 aactaccata atataagcac cttgtgctat caatggttca taacttggat aaaagcccaa
    30481 ccagttaatc gttgggaatc cttcaataac atgtcttggc atcgcaccta ataattgtaa
    30541 cttttgaata cttacgccaa gcattttgaa tcccataata aaaataaaga tcgacaacac
    30601 tctaaatata tagaaaatag gtattaattt aactataaat ctaaataata atgcaaagat
    30661 gattaaaata acgatagcta aagcaatacc aataataaaa tctttggtcg ctagctcacc
    30721 tatcatcccc atatagaaaa tgataacctc gacaccttca cgcaacacag atattaaacc
    30781 aatcgtcgct aacaatacca aattaccatt actaatcgca ttagcataca tatttttaat
    30841 catgtcattc caacgttttg catttgaacg tttgtgcatc caaacaccaa cgataaacat
    30901 taatatgacc gcaacgatac ctaatcccgc ttccatactt tcacgaagaa tgccactatt
    30961 ccctaaagtt tctacaaacg taattgctaa gataatactc agtacaagtc cggcaattgc
    31021 accaccaatc acacttgcag tccctttctt atcttttaca ttacgcgtca tggtagtcaa
    31081 tgtcattaca attaacaaca cttctagccc ttcacgtaaa aagataatca tcacatcgac
    31141 gaagctataa ctatggccaa caacctcttt aatttggtta tttaaatcta ctaaaccatc
    31201 tttcacatgt gctttattat gttcgtctaa tacactttga taatatggta ttttatcttc
    31261 aattttcgta tacaaagcac cgtctttagt ttgaatttga ccttcaacat acggccaagt
    31321 ttctataaaa tgtgtaagcg cagcatcagc atccgacaat tgattgtcgt cgatagcttt
    31381 aatcgccttc tctaacgcat catttaattg tgatacatgg tattgatcat ttgcagacgt
    31441 attacttttt ttatcgacat gatcaatatt tgatttaaaa gttgtccaag catgtgacac
    31501 ttttgccgta tctaatggtg acttatgaat tgcaattcta agttgtaata atgcgacttc
    31561 aatttgtcca tattgatttg cgtcataatt gcgaatcact gtttcattac ttgtccaaat
    31621 ctgattcaaa ctattgttca aagattctaa ttccgcttta tttttatctt taatcgcttt
    31681 tgtcatcgca gcatctttag catcgacttg ctgttgcaat agtttaattt tagaacccgc
    31741 atctttacta gccaatttct cttcataagc aattaatgac ttcgttaatt gcgaaagtgt
    31801 atctttttga ttatcattcg cttttgcatc ttcaagcttt ctcacatctg atttgacagc
    31861 attactttca ctattatctt caagcgataa tttcttaact gcacttacca cttgctcaat
    31921 tgctttctgc ttattgtcat tcgatatcga attattagaa agtgcagatt tcgcatccgt
    31981 tatcacacta tatacatcac taatactttg ttgttctgct gcctgacttt tcagtaaccc
    32041 aaagctacac accatagcag cagttattag cattgctaca aatttagtca aataatgttt
    32101 caccaaggta tcctccctta ctaacacctg gtaatactaa aaatgaagca gaacctctat
    32161 gtgtaatata ttcatttaat ttatcattac tacctaaatt attttgtata tcgataaatt
    32221 gttttgtcgc tttttgaaaa gcaataaaaa gtaagcctgt ttcgaagtta cctgtgcggt
    32281 catccgtacc atccacataa ttaaaggctc tacgtaaaat tgacgtattt gcttctttcg
    32341 ctagcctcgt atgggcatct ttatcaataa tatactcgcc atgactatct ttcgctttta
    32401 agtcaatttc atcaaactct ttcccacctg ttaacggtgc accactatgt cgtttccgac
    32461 caaatgtagc ctcttgttct tccagcgcag tacgatccca cgtttcaatg tgtatttgaa
    32521 tacgtctgac aacgcaataa gttccatgtt tcgcccatcc gtcatcaata aacacataat
    32581 ctttaagttg attattcttc ctaggattaa ttgttccatc tttaaatgcc attaaatttc
    32641 taggtgtttc cttaccttta gcagagataa aaccagattg cgcccaacgt accttaacaa
    32701 tatctcgaaa tggacgaact aaattatgaa ccgcatgaaa ggatacttgc gaatcatttg
    32761 agcatgcttg aatcataata tcaccatcgc tgtaatcgtc tattaactga tcattcggaa
    32821 aatgcggtaa atctttaaag gcatcgggaa ttttactaga taacccaatt ttcttcatca
    32881 aagacttact aatcccaaag gtaatcgtta acttgcttgc acctaatcct atcgattcac
    32941 cggtatctac tggtggcatt aaaggattgt tactcggctt gccaactgta tcaccatcca
    33001 tcatacgatc agccataacc gtccactttt taaacattgc cttaattgca tctctatctt
    33061 ttgacttcaa atctaacgca acgaaattgc atgttttttg cgtgggtgtg gtaatgcctg
    33121 gttgcacttt accataaaat tcatacgcat ctttttccgg atcttctggt gtattgaaca
    33181 ttgatttgaa agaccacatg ctaccaacac cacttgcgcc aattgcaaca ccggcaccgc
    33241 caatacctaa cattttcaaa aatgtacgtc ttgaaaattg cgtactatcg ttaacttgtt
    33301 cataatttgt cattcagtaa tcacagccat tttacttagt ggctctccta atgcattaac
    33361 agcatccgca aatgctttac ggtctttctt cgatactttt tcaaatgact cataaccgcc
    33421 gttgttatct ttatatttat ccaataattg attcacttta tcgaagttca tttggatatc
    33481 atcacttaat tttttatctt ttttctctaa aataggttta aataagtcat aaattttttg
    33541 tgcgccttca acgttcgctt taaaatcata taaatctgta tgtgaataaa tttcttcttc
    33601 acctgtgatt ttagaagttg caacttcatt taataggtca acagaacctt gtaacattaa
    33661 ttttggtatg atatctaatg tatcagcttt ggcatgcaat tcttttgcat ctttcaataa
    33721 ttgttgtgca tcttttttag tcacatcatc aattttctta tcttcgtata atgctttttc
    33781 aatcttatga tatcctgacc attctttttc ctttttctct tctttcatat ctgcaagacg
    33841 tgcatcaatt ttaggatcta aatctccaaa tgcttctgca actggttcag agcgttcata
    33901 atacatacga acttttggat ataacgcttt tgcttgggcc atatcattat tttcaatcgc
    33961 tttaacaaat ttttctgtac cttttaaaaa ttcatttaac tgttcatcag tatatttatc
    34021 atatgctttt gttgcttgtt ttaattctgc tttaacacca tcatcttttt tcgatgtctt
    34081 tgaatcatcc ttcttactat cgtcgttccc acatgcagca attaataacg ttgatgctaa
    34141 taatagcgtt gttaactttt tcattttgac acctcattat agaaattcta ttatccccat
    34201 agatgtgttc ttataaatat tattttcgca ttccctacac gctaaaataa tacattacac
    34261 ttaaaacggc tgttttaaag catcctccca taaacatcat ctagttgata ataattatca
    34321 aaatcattat attgttataa aataatactg tcaacaacct tttattttta aaaattaaat
    34381 aaaagagcgc tgccatttta aatgtcatca ctcttttgta agtctaattt gtcatttttg
    34441 gtcgtattct gattttaata aactatagat gtaactcgat gaataaatac catttagtaa
    34501 ttcattgtca cgtaacatac cttctctagt aaatcccagc ctttcaggaa tagcccggct
    34561 tttttcatta ttaactgcgg cactaatctc aattcggttt aagtcaagct cttcgaaaca
    34621 atactttatc aatgcctcaa ctgcttgtgt cataatccca tgaccctcaa attctttatc
    34681 taaatagtac cctaatgaag tttttctgtg catgtgatta atttcatgta aaccgatgac
    34741 accaactagc gttccttcat accaaatgcc acactgaaat ccattaccat cagcaaattg
    34801 caaaagtcct cttttaataa atgcacgcgt atctgatggt tgctcagttg catctaccca
    34861 aggtaaccat tccctaagtg aatttcttga acgattgact aaattgaaaa gcgcttctgt
    34921 gtcatgagct tctaaaattt ttaatgttat ttgttcattc actttcattc caaacatacc
    34981 atcacatcct cattcatttt tcatataatt ctgtaaatta tcattataat aacatattaa
    35041 tgttacgcat tgtatcttaa atttgttgtc cttc
//
LOCUS       DAKTIO010000010        32643 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.010, whole genome shotgun sequence.
ACCESSION   DAKTIO010000010 DAKTIO010000000
VERSION     DAKTIO010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 32643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32643
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.010"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..163)
                     /locus_tag="O0293_000357"
     CDS             complement(<1..163)
                     /locus_tag="O0293_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279267.1"
                     /translation="MKKQIISLGALAVASSLFTWDNKADAIVTKDYSGKSQVNAGSKN
                     GTLIDSRYLN"
     gene            353..697
                     /locus_tag="O0293_000358"
     CDS             353..697
                     /locus_tag="O0293_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN family protein"
                     /protein_id="HCY0279268.1"
                     /translation="MTTQMKIKTYLVAGIKAALLDTTGIKLASKSETTSHTYQHQALV
                     DQLHELIANTDLNKLSYLNLDAFQKRDILAAHYIAKSAIRTKNLDQMTKAKQRLESIY
                     NSISNPLHSQNN"
     gene            complement(861..2624)
                     /locus_tag="O0293_000359"
     CDS             complement(861..2624)
                     /locus_tag="O0293_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HCY0279269.1"
                     /translation="MKRISKDIWAVFKLLYQNKGRFSINALLLQLIMIFISSTYLILL
                     FNMMLKVAGQSQLTINNWTEIVSHPASVILLIIFILSVAFLIYVEFSLLVYMVYAGFD
                     RQIITFKSIFKNAFVNVRKLIGVPVIFFVIYLMLMIPIANLGLSSVLTKNIYIPKFLT
                     EELMKTTKGIIIYGTFMIAVFILNFKLIFTLPLTILNRQSLFKNMRLSWQITKRNKFR
                     LVIEIVILELIIGAILTLIISGATYLAICVDEEGDKFLVSSILFVVLKSALFFYYLFT
                     KLSLISVLVLHLKQENVLDQPGLEFKYPKPKRKSRFFIISMVLAVTCFIGYNMYLLYN
                     NTINTNISIIGHRGFEDKGVENSIPSLKAAAKANVEYVELDTIMTKDKQFVVSHDNNL
                     KRLTGVNKNISESNFKDIVGLKMRQNGHEAKFVSLDEFIETAKQSNVKLLVELKPHGK
                     EPADYTQRVIDILKKHGVEHQYRVMSLDYDVMTKLKKEAPYLKCGYIIPLQFGHFKET
                     SLDFFVIEDFSYSPRLVNQAHLENKEVYTWTINGEEDLTKYLQTNVDGIITDDPALAD
                     QIKEEKKDETYFDRSIRILFE"
     gene            complement(2960..3715)
                     /gene="pflA"
                     /locus_tag="O0293_000360"
     CDS             complement(2960..3715)
                     /gene="pflA"
                     /locus_tag="O0293_000360"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HCY0279270.1"
                     /translation="MLKGHLHSVESLGTVDGPGLRYILFTQGCLLRCLYCHNPDTWKI
                     SEPSREVTVDEMVNEILPYKPYFDASGGGVTVSGGEPLLQMPFLEKLFAELKENGVHT
                     CLDTSAGCANDTKAFQRHFEELQKHTDLILLDIKHIDNDKHIRLTGKPNTHILNFARK
                     LSDMKQPVWIRHVLVPGYSDDKDDLIKLGEFINSLDNVEKFEILPYHQLGVHKWKTLG
                     IAYELEDVEAPDDEAVKAAYRYVNFKGKIPVEL"
     gene            complement(3738..5987)
                     /gene="pflB"
                     /locus_tag="O0293_000361"
     CDS             complement(3738..5987)
                     /gene="pflB"
                     /locus_tag="O0293_000361"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCY0279271.1"
                     /translation="MLETNKNHATAWQGFKNGRWNRHVDVREFIQLNYTLYEGNDSFL
                     AGPTEATSKLWEQVMQLSKEERERGGMWDMDTKVASTITSHDAGYLDKDLETIVGVQT
                     EKPFKRSMQPFGGIRMAKAACEAYGYELDEETEKIFTDYRKTHNQGVFDAYSREMLNC
                     RKAGVITGLPDAYGRGRIIGDYRRVALYGVDFLMEEKMHDFNTMSTEMSEDVIRLREE
                     LSEQYRALKELKELGQKYGFDLSRPAENFKEAVQWLYLAYLAAIKEQNGAAMSLGRTS
                     TFLDIYAERDLKAGVITESEVQEIIDHFIMKLRIVKFARTPDYNELFSGDPTWVTESI
                     GGVGIDGRPLVTKNSFRFLHSLDNLGPAPEPNLTVLWSVRLPDNFKTYCAKMSIKTSS
                     IQYENDDIMRESYGDDYGIACCVSAMTIGKQMQFFGARANLAKTLLYAINGGKDEKSG
                     AQVGPNFEGINSEVLEYDEVFKKFDQMMDWLAGVYINSLNVIHYMHDKYSYERIEMAL
                     HDTEIVRTMATGIAGLSVAADSLSAIKYAQVKPIRNEEGLVVDFEIEGDFPKYGNNDD
                     RVDDIAVDLVERFMTKLRSHKTYRDSEHTMSVLTITSNVVYGKKTGNTPDGRKAGEPF
                     APGANPMHGRDQKGALSSLSSVAKIPYDCCKDGISNTFSIVPKSLGKEPEDQNRNLTS
                     MLDGYAMQCGHHLNINVFNRETLIDAMEHPEEYPQLTIRVSGYAVNFIKLTREQQLDV
                     ISRTFHESM"
     gene            6575..7543
                     /locus_tag="O0293_000362"
     CDS             6575..7543
                     /locus_tag="O0293_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCY0279272.1"
                     /translation="MKSKIYILLLFLIFLSACANTRHSESDKNVLTVYSPYQSNLIRP
                     ILNEFEKQEHVKIEIKHGSTQVLLSNLHNEDFSERGDVFMGGVLSETIDHPEDFVPYQ
                     DTSVTQQLEDYRSNNKYVTSFLLMPTVIVVNSDLQGDIKIRGYQDLLQPILKGKIAYS
                     NPNTTTTGYQHMRAIYSMHHRVSDVHQFQNHAMQLSKTSKVIEDVAKGKYYAGLSYEQ
                     DARTWKNKGYPVSIVYPIEGTMLNVDGIALVKNAHPHPKRKKLVQYLTSRSVQQRLVA
                     EFDAKSIRKDVSEQSDQSIENLKNIPLIPKSKLPDIPHHKFLEMIQ"
     gene            7540..9096
                     /locus_tag="O0293_000363"
     CDS             7540..9096
                     /locus_tag="O0293_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCY0279273.1"
                     /translation="MTAYKPYRHQLRRSLFASTIFPVFLVIIIGLVSFYAIYIWIEHR
                     TIHQHVDESQSSLHHTEKQIQTFITQHNNSFQELDLTNHHDVTATKRELLKLIHQQPA
                     TLYYELSGPNQFITNNYEHLNTKNMYLFSTHQLKFKNSTYMLKIYMANTPRLSEIKKD
                     NRQFALIVDQYDNILYANDDRFTIGEKYRPQQFGFMNESVKLNHADHRLIIYKDIHEN
                     IEDGITLLIVMAVVLVLLVIFGFISADNMAKRQTKDIETIIQKIYYAKNRHLGTYTPL
                     KNNSELEEINNYIYDLFESNEQLIHSIEHTERRLRDIQLKEIERQFQPHFLFNTMQTI
                     QYLITLSPKLAQTVVQQLSQMLRYSLRTNSHTVELNEELNYIEQYVAIQNIRFDDMIK
                     LHIESSEEARHQTIGKMMLQPLIENAIKHGRDTESLDITIRLTLARQNLHVLVCDNGI
                     GMSSSRLQYVRQSLNNDVFDTKHLGLNHLHNKAMIQYGSHARLHIFSKRNQGTLICYK
                     IPLSRGNVDV"
     gene            9089..9847
                     /locus_tag="O0293_000364"
     CDS             9089..9847
                     /locus_tag="O0293_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0279274.1"
                     /translation="MFKVVICDDERIIREGLKQIIPWGDYHFNTIYTAKDGVEALSLI
                     QQHQPELVITDIRMPRKNGVDLLNDIAHLDCNVIILSSYDDFEYMKAGIQHHVLDYLL
                     KPVDHAQLEVILGRLVRTLLEQQSQNGRSLASCHDAFQPLLKVEYDDYYVNQIVDQIK
                     QSYQTKVTVSDLIQHIDVSESYAMRTFKDHVGITIVDYLNRYRILQSLQLLDRHYKHY
                     EIADKVGFSEYKMFSYHFKKYLQMSPSDYCKQAK"
     gene            complement(10206..11585)
                     /gene="uhpT"
                     /locus_tag="O0293_000365"
     CDS             complement(10206..11585)
                     /gene="uhpT"
                     /locus_tag="O0293_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCY0279275.1"
                     /translation="MNFFDIHKIPNKGIPLSVQRKLWLRNFMQAFFVVFFVYMAMYLI
                     RNNFKAAQPFLKEEIGLSTLELGYIGLAFSITYGLGKTLLGYFVDGRNTKRIISFLLI
                     LSAITVLIMGFVLSYFGSVMGLLIVLWGLNGVFQSVGGPASYSTISRWAPRTKRGRYL
                     GFWNTSHNIGGAIAGGVALWGANVFFHGNVIGMFIFPSVIALLIGIATLFIGKDDPEE
                     LGWNRAEEIWEEPVDKENIDSQGMTKWEIFKKYILGNPVIWILCVSNVFVYIVRIGID
                     NWAPLYVSEHLHFSKGDAVNTIFYFEIGALVASLLWGYVSDLLKGRRAIVAIGCMFMI
                     TFVVLFYTNATSVMMVNISLFALGALIFGPQLLIGVSLTGFVPKNAISVANGMTGSFA
                     YLFGDSMAKVGLAAIADPTRNGLNIFGYTLSGWTDVFIVFYVALFLGMILLGIVAFYE
                     EKKIRSLKI"
     gene            11818..12312
                     /locus_tag="O0293_000366"
     CDS             11818..12312
                     /locus_tag="O0293_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HCY0279276.1"
                     /translation="MILSILLIFFCIRLVSLKISINHSKQLKADGAVEYGVKNSKFLA
                     ITHVLIYVLAGVEAFINKDTFSFANGIGLVILIFAYIMLFMVIKTLGGIWTLKLFILP
                     NHPIIKSGLYKITKHPNYFLNIIPELIGVLLLTHATYTTILLVPYAYFLYVRIKQEEK
                     LMNI"
     gene            complement(12674..13642)
                     /locus_tag="O0293_000367"
     CDS             complement(12674..13642)
                     /locus_tag="O0293_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCY0279277.1"
                     /translation="MKIGVFSVLFYDKNFEDMLDYVSESGLDMIEVGTGGNPGDKFCK
                     LDELLENEDKRQAFMKSITDRGLQISGFSCHNNPISPDPIEAKEADETLRKTIRLANL
                     LDVPVVNTFSGIAGSDDTAKKPNWPVTPWPTAYSEIYDYQWNEKLIPYWQDLAEFAKE
                     QDVKIAIELHAGFLVHTPYTMLKLREATNEYIGANLDPSHLWWQGIDPIAAIRILGQA
                     NAIHHFHAKDTYINQENVNMYGLTDMQPYGNVATRAWTFRTVGYGHSPYVWADIISQL
                     IINGYDYVLSIEHEDPIMSVEEGFQKACQTLKSVNIYDKPADMWWA"
     gene            complement(13697..14737)
                     /locus_tag="O0293_000368"
     CDS             complement(13697..14737)
                     /locus_tag="O0293_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCY0279278.1"
                     /translation="MTKLKVGVIGVGGIAQDRHIPALLKLKDTVSLVAVQDINTVQMI
                     DVAKRFNIPHAVETPSELFKLVDAVVICTPNKFHADLSIEALNHGVHVLCEKPMAMTT
                     EECDRMIEAANKNHKLLTVAYHYRHTDVAITAKKAIESGVVGKPLVARVQAMRRRKVP
                     GWGVFTNKALQGGGSLIDYGCHLLDLSLWLLGKDMVPHEVLGKTYNQLSKQPNQINDW
                     GTFDHTKFDVDDHVTSYMTFANRASMQFECSWSANIKEDKVHVSLSGEDGGINLFPFE
                     IYEPRFGTIFESKANVEHNEDIAGERQARNFVNACLGIEEIVVKPEEARNVNALIEAI
                     YRSDLDNKSIQL"
     gene            complement(14762..15838)
                     /locus_tag="O0293_000369"
     CDS             complement(14762..15838)
                     /locus_tag="O0293_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCY0279279.1"
                     /translation="MTIKVGIIGCGGIANGKHMPSLQKVENVEMIAFCDVDISKAASA
                     AEAYGTDNAKVYDDYKALLKDDTIDVIHVCTPNDSHCEITVAGLHAGKHVMCEKPMAK
                     TTAEAQKMIDTAKSTGKKLTIGYQNRFRADSQFLHQAAQRGDLGDIYFGKAHAIRRRA
                     VPTWGVFLDEEAQGGGPLIDIGTHALDLTLWMMDNYEPESVMGSTFHKLNKQHHAANA
                     WGSWNPDEFTVEDSAFGFIKMKNGATIILESAWAINSLEVDEAKCSLSGTKAGADMKD
                     GLRIHGEDMGTLYTKHVELENKGVDFYEGNEVDEAEEEAKAWIDAVVNDTEPVVKPEQ
                     AMVVTKILEAIYQSAKSGKAIYFE"
     gene            complement(15912..16751)
                     /locus_tag="O0293_000370"
     CDS             complement(15912..16751)
                     /locus_tag="O0293_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCY0279280.1"
                     /translation="MTKKKNILKAIGIYSFIAMMFVIILYPLLWTFGISLNPGTNLYG
                     AKMIPDNATFKNYAFLLFDDSSQYLTWYKNTLIVASANALFSVIFVTLTAYAFSRYRF
                     VGRKYGLITFLILQMFPVLMAMVAIYILLNTIGLLDSLFGLTLVYIGGSIPMNAFLVK
                     GYFDTIPKELDESAKIDGAGHMRIFLQIMLPLAKPILAVVALFNFMGPFMDFILPKIL
                     LRSPEKFTLAVGLFNFINDKYANNFTVFAAGAIMIAVPIAIVFLFLQRYLVSGLTTGA
                     TKG"
     gene            complement(16753..18021)
                     /locus_tag="O0293_000371"
     CDS             complement(16753..18021)
                     /locus_tag="O0293_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCY0279281.1"
                     /translation="MTKRNPKLAALLSVIPGLGQFYNKRPIKGTIFFIFFISFISVFY
                     SFLNIGFWGLFTLGTVPKLDDSRVLLAQGIISILLVAFAIMLYIINILDAYRNAERFN
                     RNEEIKDPKARMVATWDKTFPYLLISPGTFLLIFVVVFPLIFMFGVAFTNYNLYNAPP
                     RHTLEWVGLDNFKTLFTIGVWRKTFFSVITWTLVWTLVATTLQIALGLFLAIIVNHPV
                     VKGKKFIRTVLILPWAVPSFVTILIFVALFNDEFGAINNDILQPLLGVAPAWLSDPFW
                     AKVALIGIQVWLGFPFVFALFTGVLQSISSDWYEAADMDGASSWQKFRNITFPHVIYA
                     TAPLLIMQYAGNFNNFNLIYLFNKGGPPVSGQNAGSTDILISWVYNLTFEFNNFNMGA
                     VVSLIIGFIVAIVAFIQFRRTSTFKDEGGL"
     gene            complement(18024..19295)
                     /locus_tag="O0293_000372"
     CDS             complement(18024..19295)
                     /locus_tag="O0293_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCY0279282.1"
                     /translation="MSKILKCITLAVVMLLIVTACGPNRSKEDIDKALNKDNSKDKPN
                     QLTMWVDGDKQMAFYKKITDQYTKKTGIKVKLVNIGQNDQLENISLDAPAGKGPDIFF
                     LAHDNTGSAYLQGLAAEIKLSKDELKGFNKQALKAMNYDNKQLALPAIVETTALFYNK
                     KLVKNAPQTLEEVEANAAKLTDSKKKQYGMLFDAKNFYFNYPFLFGNDDYIFKKNGSE
                     YDIHQLGLNSKHVVKNAERLQKWYDKGYLPKAATHDVMIGLFKEGKVGQFVTGPWNIN
                     EYQETFGKDLGVTTLPTDGGKPMKPFLGVRGWYLSEYSKHKYWAKDLMLYITSKDTLQ
                     KYTDEMSEITGRVDVKSSNPNLKVFEKQARHAEPMPNIPEMRQVWEPMGNASIFISNG
                     KNPKQALDEATNDITQNIKILHPSQNDKKGD"
     gene            complement(19308..20405)
                     /gene="ugpC"
                     /locus_tag="O0293_000373"
     CDS             complement(19308..20405)
                     /gene="ugpC"
                     /locus_tag="O0293_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCY0279283.1"
                     /translation="MAELKLEHIKKTYDNNNTVVKDFNLHITDKEFIVFVGPSGCGKS
                     TTLRMVAGLESITSGDFYIDGERMNDVEPKNRDIAMVFQNYALYPHMTVFENMAFGLK
                     LRKVNKKEIEQKVNEAAEILGLTEYLGRKPKALSGGQRQRVALGRAIVRDAKVFLMDE
                     PLSNLDAKLRVQMRTEILKLHKRLNTTTIYVTHDQTEALTMASRIVVLKDGDIMQVGT
                     PREIYDAPNCIFVAQFIGSPAMNMLNATVEMDGLKVGTHHFKLHNKKFEKLKAAGYLD
                     KEIILGIRAEDIHEEPIFIQTSPETQFESEVVVSELLGSEIMVHSTFQGMELISKLDS
                     RTQVMANDKITLAFDMNKCHFFDEKTGNRIV"
     gene            complement(20788..21366)
                     /locus_tag="O0293_000374"
     CDS             complement(20788..21366)
                     /locus_tag="O0293_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HCY0279284.1"
                     /translation="MTKRPKRILATIIIFLSLLFTIIYIDDIQKWFNQYTDKLTQNHK
                     GQGHSKWEDFFRGSRITETFGKYQHSPFDGKHYGIDFALPKGTPIKAPTNGKVTRIFN
                     NELGGKVLQIAEDNGEYHQWYLHLDKYNVKVGDRVKAGDIIAYSGNTGIQTTGAHLHF
                     QRMKGGVGNAYAEDPKPFIDQLPDGERSLYDL"
     gene            21575..22201
                     /locus_tag="O0293_000375"
     CDS             21575..22201
                     /locus_tag="O0293_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCY0279285.1"
                     /translation="MAKVLYITAHPFNELVSNSMAAGKAFIETYQQQHPDDEVKHIDL
                     FETYIPVIDKDVLTGWGKMSNGETLTDDEQMKVSRLSDILEEFLSADKYVFVTPMWNL
                     SFPPVVKAYIDAISIAGKTFKYSAEGPQGLLTDKKVLHIQSRGGYYTEGPAADFEMGD
                     RYLRTIMTFLGVPSYETIIIEGHNAEPHKTEEIKATSINNAEKLATTF"
     gene            22391..23164
                     /locus_tag="O0293_000376"
     CDS             22391..23164
                     /locus_tag="O0293_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0279286.1"
                     /translation="MKAHKIFWLNLAAIIIISIVVSGDMFLAMKWEQIHLKDGLKKVL
                     STYPIKNLETLYEIDGHDNPHYENNDQDTWYIESSYSVVGSDELLKEDRMLLKVDKNT
                     HKITGEYDTTTNDKKNATDSTYKSYPVKVVNNKIVFTKDVKDPALKQKIENNQFLIQS
                     GDLTSILNSNDLKVTHDPTTDYYNLSGKLSNDNPNVKQLKRRYNIPKNASTKVELKGM
                     SDLKGNNHQDQKLYFYFSSPGKDQIIYKESLTYNKISEH"
     gene            complement(23462..25468)
                     /gene="ggt"
                     /locus_tag="O0293_000377"
     CDS             complement(23462..25468)
                     /gene="ggt"
                     /locus_tag="O0293_000377"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCY0279287.1"
                     /translation="MVINLNDKQTKTSKEGLISVSHPLAAKIGKDVLDQGGNAMDAVI
                     AIQLALNVLEPFASGIGGGGYLLYYEQSTGSITAFDARETAPEHVDKQFYLDDSGEYK
                     SFFDMTTHGKTVAVPAIPKLFDYIHKRYAKLSLEDLINPAIELAIEGHAANWATEKYS
                     RQQHARLTKYHETAQVFTHENQYWREGDWIVQPELGKTFQILREQGFNAFYKGDIAKQ
                     LVNVVKACGGTITLEDLAKYDIQIKAPISATFKDYDIYSMGPSSSGGITVIQILKLLE
                     HVDLPSMGPRSVDYLHHLIQAMHLAYSDRAQYLADDNFHEVPVQSLIDDDYLKARSTL
                     IDSNKANIDIEHGVVSDCISHTDVEENHTETTHFCVIDKEGNIASFTTSIGMIYGSGI
                     TIPGYGVLLNTTMDGFDVVDGGINEIAPYKRPLSNMAPTIVMYHGKPILTVGAPGAIS
                     IIASVAQTLINVLVFGMDIQQAIDEPRIYSSHPNRIEWEPQFSQSTILALIAHGHAME
                     HKPDAYIGDVHGLQVDPTTYEASGGSDDTREGTVMGGEVLVIRKQPLPYRQMYDSDGF
                     RLYFNDVQLPLLADQVRWMHDKYWVDESVVRIIFPEVSAHIEDLRSYENAGENYIDIA
                     WLARKYAYQVTLKDDGLYLTDDTYTSVKRNTNAYYRYDRDSITR"
     gene            complement(25506..27281)
                     /locus_tag="O0293_000378"
     CDS             complement(25506..27281)
                     /locus_tag="O0293_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCY0279288.1"
                     /translation="MKKIISIAIIVLALVLSGCGVPTKSEVAQKSSKVEVKGERPTIH
                     FLGQASYENDMNIVKDQLENAGFNVKMNIQPDYGSYRTQRQAGNYDIQIDDWMTVFGD
                     PNYAMTALFSSTGSNSLLKDKHVDQLLNKASTQNEADVKQTYKQIEDEVVFDKGYMAP
                     LYGSKKNLVYDNKVLDKNSVGLPNSRALIWQQFDYNNSRERDTRPLVMTQQDGEIPTL
                     DPIRSIAPSVYSINMNMYTRLLLLDENDHLTTKGSLSHDYAVNKDNKAFYFLLRDDDY
                     FAKVVNGQARNTGERVSAEDVKFSLDRARDKKSVPNNNTYNMHKHINDIKILKDEDID
                     QLRKEKDKDDKSIYDKLIKAYNVKSLTTDGQKVNNKDGIYQIVKITTDQSMPREVNYL
                     THSSAGILSKKFVNQVNQEYPKGYGDSSTIPANSDGKNALYASGAYIMTQKNAYQATF
                     QRNPGFNETEKGSYGPAKIKNITLKFNGDPNNALSELRNHSIDMLADVNQKHFDLIKS
                     DKNLSIIRKNGRKSVFLMLNIKKGIFKTHPNLRQAVVNAIDQDQFIKFYRGDKFKIAS
                     PITPLVDTGNEQRQDLEKVEKAINQ"
     gene            complement(27298..28461)
                     /locus_tag="O0293_000379"
     CDS             complement(27298..28461)
                     /locus_tag="O0293_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0279289.1"
                     /translation="MAQLNSKIASLKLFASYAIATYILVILTSALNLFKGYVADTFYI
                     AETLLIVLTIILIIILTTEQTWKHHDLWRRIVEVLLLLMTLTGNVFTLLMFVSIRRYQ
                     RTSQIHSYNGWESFIRKTTRHRIAIIGLLILVYMLTLSIVSQFTFDTTLATKNQFNAL
                     LHGPSLAYPFGTDDFGRDLFTRVVVGTKLTFSISIISVVIAVIFGVLLGTIAGYFNHI
                     DNLIMRILDVVFAIPSLLLAVAIIASFGASIPNLIIALSIGNIPSFARTMRASVLEIK
                     RMEYVDAARITGENTWNIIWRYILPNAIAPMIVRFSLNIGVVVLTTSSLSFLGLGVAP
                     DVAEWGNILRTGSNYLETHSNLAIVPGVCIMFVVLAFNFIGDAVRDALDPRIH"
     gene            complement(28467..29945)
                     /locus_tag="O0293_000380"
     CDS             complement(28467..29945)
                     /locus_tag="O0293_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0279290.1"
                     /translation="MNCTFRLLTSNKFDMMLCLPIHKCVNLFYTFVYHSNLLKIILQG
                     VIIIITYLESFKSLYHKAFYKFVLSVLSLPIFLYAVIKFFFSAKRKNFYANNSEISEI
                     EQALHQKYKYLSQQKSSTQIHKEALKIFKAQSSNTSSKNIEQAHFSTYFENVLFHKFI
                     MIKVILALPMFILLTFYLQPLVRYIFERIVMAVIVIIGVIVSVFTILYFSPLDAAYSI
                     LGQNATKAQIHQFNVLHHLNEPYFIQLWDTIKGVFTFDLGTTYKGNEVVTKAVGERIP
                     ITIIVAVLALMVALIIAIPIGIISAMKRNSWLDITLMIIALIGLSIPSFWQGLLFILA
                     FSLKLDILPPSYMPEHPISLILPVLVIGTSIAASITRMTRSSVLEVMRSDYVLTAYAK
                     GLSTTQVVIKHILKNAIIPIVTLVGLLVAELLGGSAVTEQVFNINGIGRYIVQKQLIP
                     DIPAVMGGVVYISIVISLANLIIDIFYALIDPKLRSEINERK"
     gene            29902..31494
                     /locus_tag="O0293_000381"
     CDS             29902..31494
                     /locus_tag="O0293_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0279291.1"
                     /translation="MSNLLEVNSLNVQFNYDETTVQAVKNVSFELRKKHILGIVGESG
                     SGKSITAKSILGLLPDYPDHTLTGEIIFNGQSLNNLSTSALQQIRGKDISMIFQDPLS
                     SLNPRLTIGKQITEVIFQHKRVSKSEAKSMTIDILEKVGIKHATRQFDAYPHELSGGM
                     RQRVMIAMALILKPQILIADEPTTALDASTQNQLLQLMKSLYEYTETSIIFITHDLGA
                     VYQFCDDVIVMKDGSVVESGTVESIFKSPQHTYTKRLIDAIPDIHQTRPPRPLNNDIL
                     LKFDRVSVDYTSPSGSLYRAVNDINLAIRKGETLGIVGESGSGKSTLAKTVVGLKEVS
                     EGFIWYNELPLSLFKDDELKSLRQEIQMIFQDPFASINPRFKVIDVIKRPLIIHGKVK
                     DNDDIIKTVVSLLEKVGLDQTFLYRYPHELSGGQRQRVSIARALAVEPKVIVCDEAVS
                     ALDVSIQKDIIELLKQLQLDFGITYLFITHDMGVINEICDRVAVMKNGEIVELNNTED
                     IIKHPQSDYAKQLISEVAVIAK"
     gene            complement(31759..>32643)
                     /locus_tag="O0293_000382"
     CDS             complement(31759..>32643)
                     /locus_tag="O0293_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HCY0279292.1"
                     /translation="GKKIEIALKDDWSIFNVEEAQISCILNELFKDDENLDRRVLEDL
                     VGAKIINDKKYTNKNLIVANNDWDGFCESLKYKNRFHNNMINLENLAFFLDITTNYYS
                     IEEFRERFEPLYRSRIVKFITEKSELMSPPKGFATAGRLNSKWISVLYLSTKEQVSIE
                     EVKPKHNDIIYIGKVKLQKNVTKEKLKIANLTNLTSNAIKAGDDGFRKYFVNYQTLKK
                     IHKGITNPSDEQGIDYLPFQYLADYIRSLKYDGIMYESILQDGTFNFVFFDQSLFECV
                     NYERKRVSDVKYTLTKLS"
BASE COUNT        10601 a         6145 c         4966 g        10931 t
ORIGIN      
        1 aatttaaata tctgctatct attaatgtcc catttttact cccagcatta acttgtgatt
       61 tcccactata atcctttgtt actatcgcat ctgctttgtt atcccatgta aataagctag
      121 atgcaactgc taatgcgcct agcgaaatta tttgcttttt cataattttt taattcctcc
      181 aaaatgtaat tgcccaatct acattaaaga aacaaaatat taaaagacat taactatata
      241 ttaactagaa taaacaaagc attaactatc tttttgttat agttaattag ctttgcaata
      301 tacaatagtg tataattttt ttgattaatg tacatcaaag gagtaacaaa gcatgacaac
      361 acaaatgaaa atcaaaacat atttagttgc tggtattaaa gcggcgctcc ttgatacgac
      421 tggtattaaa ttagcaagca aatctgaaac tacatcacat acgtatcaac atcaagcgct
      481 tgtagatcaa ttacatgaat taatagcaaa cactgactta aataaattat cgtacctaaa
      541 tttagatgcg tttcaaaaac gcgatatttt agctgcgcac tatattgcaa aatccgctat
      601 acgcactaaa aatttggatc aaatgactaa agcgaaacaa agattagaaa gtatttacaa
      661 ttcaatttct aaccctttgc attcacaaaa caattaataa ttcaccaata aatcatgtaa
      721 gtgtttgtga cgccaaattg ccatacaaat acttgtcata tgaatataaa cgaatgaaac
      781 gattgcctat cccatagatg gcaacattaa aaagacctct gaaggtatca tcttgataac
      841 tttagaggtc tttgtttata ttattcaaac aaaattctta tagaacgatc gaagtatgtt
      901 tcgtctttct tttcttcttt aatctgatca gctaatgctg ggtcatctgt gataatacca
      961 tcaacattgg tttgtaagta tttcgttaaa tcttcttcgc cgttaatagt ccaagtatag
     1021 acttctttat tttccaagtg cgcttgatta acaagtcttg gcgaataaga aaaatcttcg
     1081 atgacaaaga aatctaatga tgtttcttta aaatgaccaa actgcaacgg aatgatataa
     1141 ccacacttga gatatggcgc ttcttttttc aacttagtca tcacatcata atccaaagac
     1201 atcacacgat attgatgttc aacaccatgc tttttcaaaa tatcaataac acgttgtgta
     1261 taatctgctg gttctttacc atgtggcttt aactctacta gtagcttcac atttgattgt
     1321 ttagccgttt caataaattc gtctaaggat acaaattttg cttcatgtcc attttgacgc
     1381 attttcaaac cgacgatatc tttgaaatta gattcagaaa tatttttatt aacacctgtt
     1441 aaacgtttca aattgttatc atgactaaca acaaattgtt tatctttcgt cataattgta
     1501 tctaactcaa cgtattcgac attcgctttt gcagcagctt tcaatgacgg aatagaattt
     1561 tcaacgcctt tatcttcgaa accacgatgg ccaataatgg agatatttgt attgatagta
     1621 ttattgtaaa gtaagtacat gttataaccg ataaaacatg tcactgcaag caccattgaa
     1681 attataaaga acctagactt ccgtttcggt tttggatatt taaattctaa gcccggttgg
     1741 tctaatacat tctcttgttt taagtgcagt actaacacac tgattaatga taatttcgta
     1801 aataaataat agaagaacaa tgcgcttttc aatacaacaa ataaaattga tgagactaaa
     1861 aacttatctc cttcttcatc tacacaaata gcaagatatg ttgctcctga aataattaat
     1921 gttaaaatcg caccaatgat gagttctaat ataactattt ctataacaag ccgaaactta
     1981 tttcgcttcg taatttgcca acttagtctc atatttttaa ataacgactg gcggtttaaa
     2041 atcgttaacg gtagagtaaa tattaattta aaatttaata taaatacagc aatcataaag
     2101 gtaccgtaaa tgattatacc tttcgtcgtt ttcataagtt cttccgttaa aaatttaggt
     2161 atgtaaatat tttttgttaa tactgaactt agtcctaggt tggcaatggg tatcattaac
     2221 attaaataaa tgacaaagaa aataactggt acacctatga gtttacgcac atttacaaag
     2281 gcatttttaa aaatggattt aaatgtaata atctgtcgat caaagccggc ataaaccata
     2341 taaactaaca atgaaaactc tacataaatc agaaaggcaa cacttaatat gaatataata
     2401 agaagtatca cactggcggg atgactaacg atttccgtcc aattgttaat cgtaagttgg
     2461 ctttgcccag ctacttttaa catcatatta aatagtaaaa ttaagtatgt actactaata
     2521 aaaatcatga ttaactgcaa tagtaaggca ttaatgctaa aacgcccttt attttgatac
     2581 agtaatttaa atactgccca tatatcttta ctaattctct tcataatcac gctccgcatt
     2641 gctttaatat taagtttcat cttaatattt ttcattactc agggtcaata aaaatttgaa
     2701 aagactcata ttcatatgca agtagcaaat aataacccat tcaacatcag ctaaatgatg
     2761 atattggaac cccaccttta acaagacatc acattcttta tcagcatagc tacttacaaa
     2821 aacgtcttcc ttcaaccatt gtagaagttg tctcatatac ttactacgtc ttgctaatac
     2881 tcaatactca attgaaaaag aagcatatgc cccttcactc ttgaagttgc atatgctttc
     2941 ttttcggtct gaattgtatt tataattcaa cgggaatttt ccctttgaag ttaacataac
     3001 ggtaggctgc tttaacagct tcatcatcgg gcgcttcgac atcttctaat tcatatgcaa
     3061 tgcccaatgt tttccactta tgaacaccta actgatgata tggcagaatt tcaaactttt
     3121 cgacgttatc aagagaatta ataaattccc ctagtttaat taaatcgtct ttatcatcag
     3181 aataaccagg cacaaggaca tgtcgaatcc atacaggttg tttcatatct gacagtttgc
     3241 gcgcgaagtt aaggatgtgt gtattaggct ttcctgtcaa tctaatatgt ttgtcattat
     3301 caatatgttt tatatctaat aatatcaagt ctgtatgttt ttgtaattct tcaaaatgcc
     3361 tttgaaatgc ttttgtatca ttagcacatc cagccgatgt gtctaagcaa gtgtgcacac
     3421 cattttcttt taattctgca aataattttt ctaagaatgg catttgtaac aatggttcgc
     3481 caccactgac tgttacaccg ccacccgatg catcaaagta tggtttgtat ggtaatattt
     3541 cattcaccat ttcatcaact gtgacttctc ttgatggctc actaattttc caagtatctg
     3601 gattgtggca atacaagcat ctaagtaagc atccttgtgt aaataatata tatcttaatc
     3661 ccggtccatc gacagtacct aaactttcga cagaatgtaa gtgtccctta agcatagtgc
     3721 tcccacctta aattttgtta catactttca tggaatgtac gagaaattac atctaattgt
     3781 tgttcacgtg ttaatttaat gaagttaaca gcgtaaccag atacacggat tgttaactgt
     3841 ggatattctt ctggatgttc cattgcatct attaatgttt cacggttaaa tacgttaata
     3901 tttaagtggt gaccacattg cattgcgtaa ccatctaaca tactagttaa gttacggttt
     3961 tgatcttctg gttctttacc taatgatttt ggtacgatac tgaatgtatt tgaaatacca
     4021 tctttacagc aatcgtaagg gatcttagct acagaactta atgaagataa tgcacctttt
     4081 tggtcacggc catgcattgg gtttgcacct ggagcaaatg gttcgccagc tttacgtccg
     4141 tctggtgtgt taccagtttt cttaccgtat acaacgtttg aagtaattgt taatacactc
     4201 attgtatgtt ctgaatcacg atatgtttta tgactacgta atttagtcat gaagcgttct
     4261 actaaatcaa ctgcaatatc atctacacgg tcgtcattgt taccgtattt agggaagtcg
     4321 ccttcgattt caaagtctac tacaagacct tcttcgttac gaattggttt aacttgtgca
     4381 tatttaattg cagataatga gtcagctgct actgataaac cagcgatacc tgttgccatt
     4441 gtacgtacaa tttctgtatc atgtaatgcc atttcaatac gttcatagct gtatttatcg
     4501 tgcatgtagt gaataacatt taatgagtta atgtaaacac ctgctagcca atccatcatt
     4561 tgatcaaatt tcttgaatac ttcgtcatat tctaatactt cgctgttaat accttcgaag
     4621 tttggaccaa cttgtgcacc agatttttca tctttaccac cattgatagc gtaaagtaat
     4681 gttttagcta agttcgcacg tgcaccgaag aattgcattt gtttaccaat tgtcatcgct
     4741 gatacacaac atgcgatacc atagtcatcg ccatagcttt cacgcataat gtcatcattt
     4801 tcatattgga tagaacttgt tttaatactc atttttgcac agtatgtttt gaagttgtca
     4861 ggtaaacgta ctgaccataa tactgttaag tttggttctg gagctggacc taagttatct
     4921 aatgagtgta agaaacggaa tgagtttttc gtaacaagtg gacgtccgtc aatacctaca
     4981 ccaccgatag attcagttac ccaagttggg tctccagaga ataattcatt gtaatcaggt
     5041 gtacgagcaa atttaacaat acgtaatttc atgatgaagt ggtcaataat ttcttgaact
     5101 tcgctttcag taataacgcc tgctttaagg tcacgttcag catagatatc taagaatgtt
     5161 gatgtacgac ctaaactcat tgctgcaccg ttttgttctt taattgcagc aaggtatgct
     5221 aagtataacc attgaactgc ttctttgaag ttttctgctg gacggcttaa atcgaaacca
     5281 tatttttgtc caagttcttt taattctttt aatgcacgat attgttctga taattcttca
     5341 cgtaaacgaa ttacatcttc tgacatttct gtagacatcg tgttgaagtc gtgcattttt
     5401 tcttccatta agaaatctac accatataaa gctacacgac gatagtcacc gataatacgt
     5461 ccacgtccgt atgcatcagg taaaccagtg attacacctg ctttacggca gttcaacatt
     5521 tctctagaat atgcatcgaa tacaccttgg ttatgtgttt tacgataatc tgtaaagatt
     5581 ttttcagttt cttcgtctaa ttcgtaaccg taagcttcac aagctgcttt cgccatacga
     5641 ataccaccga atggttgcat tgaacgtttg aatggctttt cagtttgtac acctacaatt
     5701 gtttctaaat ctttgtctaa ataaccagca tcatgagatg tgattgttga agctactttc
     5761 gtgtccatat cccacatgcc gccacgttca cgttcttctt tcgataactg cattacttgt
     5821 tcccaaagtt tagaagttgc ttctgttggt cctgctaaaa atgaatcatt accttcataa
     5881 agagtgtagt ttaattggat aaactctctt acatctacgt gtctgttcca tcttccattt
     5941 ttaaatcctt gccaagctgt tgcatgattt ttatttgttt ctaacatact aaacgcctcc
     6001 atctgtataa tgttgtgaat ttgaaatgct atgttaacgt tttcatagtc attgtagcat
     6061 gtttgttgtg atttttttca cataaattat gtacaatttt catcaaatgt atgtcaaaac
     6121 atagaaactg tactaatttg tgtaacagta atatgctaat tgcattctgt tacacaacag
     6181 ttataatgtt cactattttc attgctttaa aagttaattt aggagtatat atttgtttat
     6241 aaaaaatgat tgttgataag cgttaaatca acacttagaa agatttactt ttatatatga
     6301 agagactgga ttaaatactt ttattgacgt aaaaattcac ttttgaaccg ttcaatatct
     6361 tgccgatttt tatataacag ctacaaataa aatataacag tttgatttta cagcctcggt
     6421 aaatcgtctt gacaaacaaa aattttgtgc tatcacaaca tttgcaacgt cttaacaagt
     6481 catctataaa catttctaaa tatttaacat tacttatgcg tcatttattg ctaaaattat
     6541 tgtattaaaa tatacataga attgatggga tatcatgaaa tcaaaaattt atatcttgct
     6601 attatttctc atttttttat cagcatgcgc taatacgcgt cactctgaat ccgataaaaa
     6661 tgtattaaca gtttattctc cgtatcaatc aaacttgatt cgtccaattt taaatgaatt
     6721 tgaaaaacaa gagcatgtca aaattgaaat taaacacgga tctactcaag tactgctttc
     6781 aaacttgcat aacgaagatt tttcggagcg tggtgatgtc tttatgggtg gtgtgttgtc
     6841 agaaacaatt gatcatccag aagattttgt tccctatcaa gatacatctg taacacagca
     6901 attagaggat tatcgctcga acaataaata tgttactagt tttctattaa tgccaacagt
     6961 tatagtagtg aattcagatt tacaaggaga tattaagatt cgaggttatc aagatttatt
     7021 acaacctata cttaaaggta aaattgcgta ctcaaatcca aatacaacaa cgacaggcta
     7081 tcaacatatg cgtgctattt atagcatgca tcatcgagta agtgatgtgc atcaattcca
     7141 aaaccatgcg atgcaactgt caaagacgtc taaagtcatt gaagatgttg caaaaggtaa
     7201 atattacgca ggtctaagct acgaacaaga tgcacgcaca tggaaaaaca aaggttatcc
     7261 tgtatcaatc gtttatccaa ttgaaggaac aatgttaaat gttgatggta ttgctttagt
     7321 taaaaatgca caccctcatc ctaagcgtaa aaagttagtg caatatttaa caagccgctc
     7381 agtacaacaa cgattagttg cagagttcga tgccaagtca attcgaaagg atgttagtga
     7441 acaaagcgat cagtctatcg aaaatcttaa gaacatacct ttaataccga aatctaaatt
     7501 accggatatt ccacatcata aatttttgga gatgattcaa tgacggcata caaaccttat
     7561 agacatcaat taagacgatc gttatttgcg tctactattt ttccagtatt tttagtcatt
     7621 attattggac tagtaagctt ctatgccatc tatatttgga ttgagcatcg tacgattcat
     7681 caacatgttg atgaaagtca gtcatcttta catcatactg aaaagcagat tcagacattt
     7741 attacacagc ataataattc gtttcaagag ctcgatttaa caaatcatca tgatgtgacc
     7801 gctacaaaac gtgaattatt aaaattaata caccaacagc ccgcaacact ttattatgaa
     7861 ttatctggac caaatcagtt tataaccaat aattatgaac atttaaacac taaaaatatg
     7921 tatttatttt caacgcatca gcttaaattc aaaaatagta catatatgct taaaatttat
     7981 atggctaaca ccccacgttt aagtgaaatt aagaaagata accgacaatt cgcacttatc
     8041 gttgatcaat atgacaatat tttatacgcc aacgatgacc gttttactat aggtgaaaag
     8101 tatagaccac aacaattcgg atttatgaat gaatctgtaa aattaaacca tgctgaccat
     8161 cgtctcatta tttataaaga tatacatgaa aacattgaag atggtatcac gttgttaatt
     8221 gttatggccg tagttcttgt tttactagta atatttggtt tcattagcgc agataatatg
     8281 gctaaacgac aaacaaaaga tatcgaaacg attattcaaa aaatttacta tgccaaaaat
     8341 cgtcatctag gtacatatac gcctttaaaa aacaacagtg aactagaaga aattaataat
     8401 tatatttacg atttatttga atcaaatgag caactgatcc attctatcga gcatacagaa
     8461 cgtcgcttgc gtgatattca gttaaaggaa atagaacgcc aattccagcc tcatttccta
     8521 ttcaatacga tgcaaacaat acaatattta attacgttat ctccgaaatt agcacaaact
     8581 gtggtccaac aattatcgca aatgttacgt tactcattac gtacaaactc acatacagtg
     8641 gaattaaacg aagagttgaa ctacattgaa cagtatgttg caatacaaaa catccgtttt
     8701 gatgacatga ttaagctcca tattgaaagt tctgaagaag cgcgtcacca gacaattggt
     8761 aaaatgatgc ttcagccact catagaaaat gctattaaac atggtagaga tactgagtca
     8821 ttggacatta ctatacgcct gacattggca cgtcagaatt tacatgtttt agtttgcgac
     8881 aatggtatcg ggatgtcgtc atctcgcctg caatatgtac gccaatctct taataatgat
     8941 gtatttgata ctaagcattt gggattaaat catttacaca ataaagcaat gattcaatat
     9001 ggttcacatg cacgactcca tattttttca aagcgtaatc aaggtacgtt aatttgttat
     9061 aaaataccac tttcaagggg gaatgtagat gtttaaggta gttatttgtg atgatgaacg
     9121 aattattcga gaaggattaa agcaaatcat tccttgggga gactatcatt tcaatacaat
     9181 atacactgct aaagatggtg ttgaagcatt atcactaatt caacaacatc agccagaatt
     9241 agtaattact gatattcgta tgccacgtaa aaatggcgtc gatttactca atgatattgc
     9301 gcatctagat tgtaatgtca tcatattatc gagttacgat gactttgaat atatgaaagc
     9361 cggcattcaa catcatgttc ttgattattt attaaaacca gtagaccatg cacaattaga
     9421 agttatttta ggaagacttg tccgtacctt attagagcaa caatcacaga acggccgtag
     9481 cttagcatct tgtcatgatg ccttccaacc actattaaaa gtagaatacg atgattatta
     9541 tgtaaaccag attgtggatc aaattaagca atcttatcaa acgaaagtga ctgtttcaga
     9601 tttaattcaa catatcgatg ttagtgaatc gtatgcgatg cgaacattta aagatcatgt
     9661 tggtattaca attgtcgatt atttaaatcg ctatcgtatt ttacaatctc tgcaattgtt
     9721 agatcgacat tacaaacact atgaaattgc agacaaagta ggcttttcag agtataaaat
     9781 gtttagctat cattttaaaa aatatttaca aatgtcgcca agtgattatt gtaagcaagc
     9841 aaaatagatt agcacataac taatgattat ggcgcctcca ttttgttcta tgaaaaagcg
     9901 gactgataga gaagttttca cttttctttc agtccgcttt ttagagagtt taaatataaa
     9961 acaatactcc ctacaatatt gacaccgcta tatgtcttta ttgtaaggag taaattcatt
    10021 gagtcaccct aagtcgggca aataaggata cttcataaca gggaattatg atctgccccg
    10081 agacatagag atgatatatt tgcaagggta gaagcagtat ctttcatctc ttcacgcaca
    10141 tgccgtcggg gcaggattgc caactatatt gcaatagatt ggcgatactt ttaatccgat
    10201 ttatattaaa tttttaaact tctaattttc ttttcttcat agaaagcaac gattcctaat
    10261 agaatcatgc ctaggaataa tgcaacatag aagacgatga aaacatctgt ccatccactt
    10321 aatgtatatc caaagatgtt taaaccgtta cgtgttggat cagcaatagc cgccaaacca
    10381 actttcgcca ttgagtcacc gaatagatac gcgaatgaac ctgtcattcc gtttgctaca
    10441 ctgatggcat ttttaggaac aaaaccagtc aatgatacac caattaataa ttgcggacca
    10501 aagattaacg cacctaatgc aaacaatgaa atgttaacca tcatgacact tgtagcattt
    10561 gtgtagaata agacaacaaa tgtaatcata aacatacagc caatagctac aattgcacga
    10621 cgacctttta ataagtctga tacgtagccc cataataaac ttgcaactaa tgcaccaatt
    10681 tcaaagtaga atatcgtatt aactgcatcg cctttactaa agtgtaaatg ctctgacaca
    10741 tataacggtg cccagttatc aataccgatt cgtacaatgt atacaaagac gtttgaaaca
    10801 catagaatcc atataacagg atttcccagg atatattttt taaagatctc ccatttcgtc
    10861 ataccttgag aatcaatatt ttctttatcg accggctctt cccaaatttc ttcagcacga
    10921 ttccatccta attcttccgg atcatctttt ccgataaata atgttgcgat accaataagt
    10981 aatgcaatca ccgatgggaa aatgaacatc cctataacat ttccatggaa gaatacatta
    11041 gcaccccaaa gtgcaacacc acctgctatg gcaccaccga tattatgtga tgtattccag
    11101 aatcctaagt atcggccacg tttcgttctt ggcgcccatc ttgaaatcgt tgaataactt
    11161 gcaggtccac caactgattg gaacaccccg ttaagtcccc aaagtacaat taataatccc
    11221 attacagaac caaagtaact taaaacaaat cccataatta aaactgtaat cgcagataag
    11281 ataagtaaga acgagataat acgttttgtg ttacgtccat cgacaaaata tccaagtaat
    11341 gtttttccta aaccgtacgt gatactaaat gctaatccga tataaccaag ttctaatgta
    11401 gataatccaa tttcctcttt taaaaacggt tgtgccgcct taaagttgtt tcgaattaaa
    11461 tacatagcca tataaacaaa gaacactacg aagaaagctt gcatgaagtt tctaagccat
    11521 aatttacgtt gtaccgataa tggaatgcct ttgttcggaa tcttatggat atcaaaaaag
    11581 ttcatctctg tcacctcaat cattttcgtt aagccaaata tatcacgtcg gtgtaagcgc
    11641 ttaatatcct taaaattatt aaattatcca aaatactgaa cataaacaca tacttagaaa
    11701 catatttatg tactaaaatt aataaatagt taatagtatt tgacaagctt tatctataaa
    11761 atactttatg ttgtcaaaat caacgcttat acatatgaca taatgacgga ggacatcatg
    11821 attctatcta tattacttat ctttttctgt atcagacttg tcagcttaaa gatatctatt
    11881 aatcactcaa aacaattaaa agcagacggt gcagttgaat atggcgttaa aaattcgaag
    11941 tttttagcaa taacacacgt tttaatttat gtattggctg gtgtagaggc atttattaat
    12001 aaagatacat ttagctttgc aaatggtatt ggcttagtta tattaatctt tgcttatatc
    12061 atgttattta tggttattaa aactttaggt ggtatttgga cattaaaatt attcatttta
    12121 cctaaccatc ctattattaa atctggatta tataaaatta caaaacaccc aaattacttc
    12181 ttaaacatca ttcctgaatt aatcggtgta ttattattaa cacacgcaac atatacaact
    12241 attttattag taccgtatgc gtatttctta tatgtacgta ttaaacaaga agagaaatta
    12301 atgaatattt aaaaagatga aagcctgtca tcactttagt ctacatcgac gtccatcttt
    12361 aaagtgcatt ctgaaatatt atattcagaa acctttaaag tatgtcgtgt ctatgaccat
    12421 tgtgacaaca ggcttttttt tattatcata tacctcacta ttgaaaaggt tatatactct
    12481 tttcaatggt gaggattgtt actgtgaatg ggtatgacca tcgctatata cttatataaa
    12541 gtacaccttc tttacaaaca atgacttgat ttaaggtgtg aaatttttgg caatggcttg
    12601 gcgacatatg catttattca acactgccac cagtcttact tcagacaaac ttcagactaa
    12661 cctcgagttc gtattatgcc caccacatgt ctgctggctt gtcgtaaata ttaacagatt
    12721 tcaaagtttg acaagctttt tggaaacctt cttctactga cataatagga tcttcatgtt
    12781 caatacttaa tacataatca tatccattaa taataagttg acttatgata tctgcccata
    12841 catatggact atgtccataa ccaactgtac ggaatgtcca tgctcttgtc gcaacgttac
    12901 catatggttg catatcagtt agaccataca tatttacatt ttcttgatta atatacgtat
    12961 ctttagcatg gaagtgatga attgcatttg cttggcctaa tatgcgaatc gcagcaattg
    13021 ggtcaatacc ttgccaccat agatgactag gatctaagtt agcaccgata tattcatttg
    13081 tagcctcacg taacttcaac attgtatatg gtgtatgcac taaaaatcct gcatgcaact
    13141 ctatggcaat ttttacatct tgctcttttg caaactcagc taaatcttgc caatatggta
    13201 tcaacttttc attccactga taatcataaa tttcagagta ggctgttggc caaggtgtaa
    13261 caggccaatt aggcttttta gcggtatcat ctgatcctgc aatgccagaa aatgtattaa
    13321 caactggcac gtctaataga tttgctaaac ggattgtttt acgtaacgtt tcatcggctt
    13381 ctttcgcttc tatcggatct ggagaaattg gattgttatg acaactgaaa ccacttattt
    13441 gtaagcctct gtctgtgatt gacttcataa atgcttggcg cttgtcttca ttttctaaca
    13501 actcatctaa cttacaaaat ttatctcctg ggttaccacc tgttccaact tcaatcatat
    13561 ccaatccaga ttctgagaca taatctaaca tatcttcaaa atttttatcg taaaataata
    13621 ctgaaaatac acctattttc attgaaaagc actccttctt tttatattga gaattttgta
    13681 tcatatatga taatcattaa agttgtatgc tcttgttatc aagatcgcta cgataaatcg
    13741 cttctataag ggcatttaca ttgcgtgctt cttccggttt caccacaatc tcttctatcc
    13801 ctaaacacgc attgacaaag ttacgcgcct gtctctcacc agcaatgtct tcgttatgct
    13861 caacattagc tttgctttca aaaatagttc caaagcgggg ctcatatatt tcaaatggaa
    13921 ataaattgat accgccatct tctcctgata aactaacgtg aaccttatct tctttgatat
    13981 ttgcagacca cgaacattca aactgcatgc ttgctcgatt ggcaaatgtc atataactag
    14041 taacatgatc atcgacatca aatttagtat gatcaaatgt tccccaatca ttaatttgat
    14101 tcggttgttt gctcaattga ttatatgttt ttcctagcac ttcatgcggc accatatctt
    14161 tacctagtag ccacaaagat aagtctaaca agtggcaacc ataatcgatt aaactaccgc
    14221 caccttgcaa cgctttattg gtaaaaacac cccagccagg cactttacgc ctacgcatcg
    14281 cttgtacacg tgctactaaa ggtttaccaa ccacacctga ttcaattgct tttttagcag
    14341 taattgccac atctgtgtga cgataatgat atgcgacagt taataatttg tgatttttat
    14401 tagccgcttc aatcatgcga tcacactctt ccgtcgtcat cgccattggc ttttcacaca
    14461 atacatggac accatggttc aatgcttcta tagaaagatc agcatggaat ttattaggtg
    14521 tacaaatgac caccgcatca acaagtttaa acagctcgct aggtgtctca actgcatgag
    14581 gtatattaaa gcgcttcgca acatcaatca tctgcactgt attaatatct tgtactgcaa
    14641 ctaatgagac tgtgtctttg agtttcagca atgctggaat atgacggtct tgtgcaatac
    14701 caccaacacc tatcacacca acttttaatt ttgtcatgat gtgcctcctt accgtatgat
    14761 gttattcaaa gtaaattgct ttgcctgatt ttgcagactg ataaatcgct tcaagaattt
    14821 ttgtaactac cattgcttgt tccggtttca caactggttc agtatcattt acaactgcat
    14881 caatccaagc ttttgcttct tcttcagctt catccacttc attaccttca taaaagtcga
    14941 cgcctttgtt ttccaattca acgtgtttgg tataaagtgt acccatgtct tcaccatgaa
    15001 tacgtagacc atctttcata tcagcacctg ctttagttcc tgataatgaa cattttgcct
    15061 catccacttc taaagaatta atcgcccaag cggattctaa aatgatcgtc gctccattct
    15121 tcattttaat aaatccaaac gcagaatctt caactgtaaa ttcatctgga ttccatgaac
    15181 cccaagcgtt tgccgcatga tgctgtttat ttaatttatg gaatgttgaa cccatcactg
    15241 attctggttc ataattatcc atcatccata acgttaaatc taaagcgtgt gtaccgatat
    15301 cgattaatgg tcctccacct tgagcttctt cgtctagaaa gacaccccat gttggtactg
    15361 ctcgacgacg aatggcatgt gcctttccga agtaaatgtc tcctaagtcg ccacgttgcg
    15421 ctgcttgatg taaaaattga ctatctgctc ggaaacgatt ttgataacct attgttaatt
    15481 ttttacctgt tgatttagct gtatctatca ttttttgagc ttctgctgtc gttttagcca
    15541 ttggtttttc acacatcaca tgtttaccag catgcaaccc tgctacagta atttcacaat
    15601 gcgagtcatt tggcgtacaa acatggataa catcaatcgt gtcatctttt aacaatgctt
    15661 tgtaatcatc ataaaccttt gcattgtcag ttccgtatgc ttctgccgca ctcgctgctt
    15721 tcgaaatgtc tacgtcacaa aatgcgatca tttcaacatt ttcaactttt tgtaaacttg
    15781 gcatgtgctt gccattcgca ataccaccac acccaatgat tccaacttta atcgtcatca
    15841 taacgtcctc cttttaatct ttatcgtcat tagtggttct ttatcaattc tgccccactc
    15901 ctaatttcaa actaaccttt tgtcgcacct gttgttaaac ctgatactaa atagcgttgc
    15961 aagaacaaga atacgattgc tataggtact gcaatcataa ttgcccctgc tgcaaacact
    16021 gtgaaattat ttgcatactt atcattaata aagttgaaca atccaactgc taatgtgaat
    16081 ttttcaggac ttcttaatag tattttaggt aatataaagt ccataaatgg ccccataaaa
    16141 ttgaacaaag caacaactgc taaaatcggc ttagctaatg gaagcataat ttgtaagaaa
    16201 atacgcatat gccctgcacc atcaattttg gcagattcat caagttcttt tggaatcgta
    16261 tcgaagtaac ctttcactaa aaaggcattc atcggtattg atccaccaat atataccagt
    16321 gttagtccaa ataaagaatc taataatcca attgtattta gcaaaatata gattgcgacc
    16381 attgccatta atacagggaa catttgtaaa atcaaaaatg taatcagccc gtatttacga
    16441 ccaacaaagc gatatctaga aaaagcatat gctgttaacg tgacaaatat cacactaaac
    16501 agtgcatttg cagatgctac gataagcgta tttttatacc aagtcaggta ttgactactg
    16561 tcatcgaata gtaagaatgc ataattttta aatgttgcat tgtctggtat cattttggca
    16621 ccatacaagt tcgtacctgg attaagggaa atgccaaatg tccacagtag tggatataaa
    16681 atgatgacaa acatcatcgc tataaaactg taaataccga ttgcttttaa tatgtttttc
    16741 ttctttgtca tcttataaac ctccctcatc tttaaacgta cttgtacgtc tgaattgaat
    16801 aaatgcgaca atagcaacaa taaatccaat aattaatgac acaactgcac ccatgttgaa
    16861 gttgttaaac tcaaatgtca gattatacac ccaagatatc aagatatctg tactaccagc
    16921 attctgccct gacactggtg gaccgccttt attaaataga taaataagat taaaattatt
    16981 gaaattacct gcatattgca taattaacaa tggcgctgtg gcgtaaatga catgcgggaa
    17041 tgtgatgttt ctaaactttt gccaactaga cgcaccatcc atatctgctg cttcgtacca
    17101 atctgatgaa atactttgca gtactccagt gaacagtgca aagacaaatg ggaatccaag
    17161 ccatacttga atgccgatta atgccacttt tgcccaaaac ggatcactta accatgctgg
    17221 tgctacacct aataaaggtt gcaaaatatc attatttatc gcaccaaatt catcattaaa
    17281 taacgctaca aatattaaaa ttgtcacaaa tgatggtaca gcccaaggta ggattaacac
    17341 agtacggata aatttcttac ctttgacgac agggtgattt acaataattg ccaaaaacag
    17401 ccctaatgca atttgaagtg tcgttgcaac aagcgtccat actaatgtcc aagtaataac
    17461 actgaaaaat gttttacgcc aaacgccaat tgtgaataac gttttaaagt tatctaaacc
    17521 aacccattct aatgtgtgtc tcggaggcgc gttgtataaa ttgtaatttg taaatgctac
    17581 tccaaacata aatattaatg gaaatacaac tacaaatatc aataagaatg tacctggtga
    17641 gattagtaag tatgggaacg tcttgtccca tgttgccacc atacgcgcct tcggatcctt
    17701 tatttcctca ttgcgattaa atcgttcagc attacgatat gcatctaaaa tattaatgat
    17761 atatagcatg attgcgaaag caacgagtaa gatagaaata ataccttgtg caagtaagac
    17821 acgagaatcg tctaacttag gtactgtccc taatgtgaac aatccccaaa aaccaatatt
    17881 taaaaagcta taaaaaacag aaataaaact gatgaaaaag ataaaaaata tcgtcccttt
    17941 aatgggtctt ttattataaa actgtcccaa accaggtata acagataata atgccgctaa
    18001 tttagggtta cgtttcgtca taactaatct cctttcttat cattttgtga tggatgaaga
    18061 atcttaatat tttgcgttat atcattcgtc gcctcatcta acgcttgttt aggattctta
    18121 ccatttgaaa taaatatgct tgcattgccc atcggttccc aaacttgtcg catttcagga
    18181 atattaggca tcggttcagc atgacgtgct tgcttttcaa acacttttaa atttggatta
    18241 gatgatttca cgtcaacacg tccagtaatt tcgctcattt catctgtata tttttgtaat
    18301 gtatctttac tagtgatata cagcattaaa tctttagccc aatacttatg tttactatat
    18361 tcagataaat accaaccacg tacacctaga aatggtttca taggtttgcc accatctgta
    18421 ggtaatgttg ttactcctaa atctttacca aacgtttctt gatattcatt aatgttccac
    18481 ggtccagtga caaattgtcc tacttttcct tctttaaaaa gaccaatcat gacatcatgt
    18541 gttgctgcct taggaagata ccctttgtcg taccattttt gtaatcgttc agcattcttg
    18601 acgacatgtt ttgaatttag tcctagctga tgaatatcat attcactgcc atttttcttg
    18661 aaaatataat catcattgcc gaataaaaac ggataattaa aatagaaatt tttagcatca
    18721 aataacatac cgtattgttt ctttttacta tcagttagtt tggcagcatt agcttcaact
    18781 tcttctaacg tttgcggtgc atttttcact aattttttat tataaaaaag tgcggttgtt
    18841 tcaacgatag ctggcaatgc tagttgctta ttgtcataat tcatcgcttt aagtgcttgc
    18901 ttattgaaac ctttcaactc atcttttgat aatttgattt cagcagctaa gccttgtaga
    18961 taggcacttc cagtattatc atgtgctaag aaaaagatat ctggaccttt tcctgcagga
    19021 gcgtctagcg aaatattttc tagttgatca ttttgaccaa tatttacaag ctttactttg
    19081 atgccagttt ttttagtata ttgatccgta atttttttat aaaacgccat ttgcttgtcg
    19141 ccatccaccc acatcgtaag ttggttaggc ttgtctttag aattatcttt attcaatgct
    19201 ttatcaatat cttctttcga acgattaggg ccacatgcag ttacgattaa taacattacc
    19261 acggctaacg tgatacattt taaaatttta gacatgaata ctccccctta gacgatacga
    19321 tttcctgttt tttcatcaaa aaagtgacac ttattcatat caaatgctag tgtaatcttg
    19381 tcgttcgcca tcacttgagt tcttgaatct aatttagaaa tcaattccat tccttggaat
    19441 gtgctatgta ccataatttc tgaacctaac agttcggata caactacttc agattcaaat
    19501 tgtgtctctg gagaagtttg aataaatatt ggttcttcat gaatgtcttc agctcgaata
    19561 cctaaaataa tttccttgtc taagtagcca gcagctttta acttttcaaa ttttttatta
    19621 tgtaatttaa aatggtgtgt tcctaccttc aatccgtcca tttcaactgt agcattcaac
    19681 atattcattg ctggtgagcc gataaattgc gccacaaata tgcaattagg ggcatcatat
    19741 atttctctag gtgtgccgac ttgcataatg tcgccatctt tcaaaacaac aattcgacta
    19801 gccatcgtca atgcttcagt ttgatcatgt gtaacataaa ttgtcgtagt attaagtcgc
    19861 ttatgtaatt tcaatatttc tgtgcgcatt tgtactcgaa gcttcgcatc aagattcgat
    19921 aatggttcat ccattaaaaa gactttcgca tccctaacaa tagctctgcc caaagcaaca
    19981 cgttgacgct gtccgccaga taacgctttt ggtttacgac caagatactc agttaatcct
    20041 aatatttcag ctgcttcatt aactttttgt tcaatctctt ttttatttac tttacgtagc
    20101 tttagcccaa atgccatatt ttcaaaaaca gtcatatgtg gatataatgc atagttttga
    20161 aataccatcg caatatctct attctttggt tcaacatcgt tcatgcgttc cccatcaata
    20221 taaaaatctc cagatgtgat agactctagt ccagcaacca ttcgtaatgt tgttgattta
    20281 ccacatcccg atggtccaac aaatacaatg aattctttgt cagtaatatg tagattaaaa
    20341 tctttcacta cagtattgtt gttatcatac gtctttttaa tatgctctaa ctttagttct
    20401 gccatgttat gcctcccata ctttgtttac agtttgattg tgcaatacac ttcgtgaaaa
    20461 cgtttgcaca aatacttaca taaatcatat attcagtcat tattaatgtc aatcataaat
    20521 tataaacgtt tacattactg tcagaattaa acttttatac agaaaattga tatagtgcac
    20581 aactattttc tagtgtgaca ataatgtttt ttatgtgcga tatttcactg ccaatgctaa
    20641 caaccttgtc ttacatttta agtaatatct aaatcaaaca agtatgactt agcattacga
    20701 ttttcattga aaaaagcaac cattatgtaa gtatcatact cacacaatga ttgcttaatt
    20761 ttttagactg cgggcaccct tctataacta caaatcatat aggctacgtt ccccatcagg
    20821 taactgatcg ataaacggtt ttggatcttc tgcatatgca ttacctacgc cacccttcat
    20881 tctttgaaaa tgtaaatgtg cgcctgtcgt ttgtatacct gtattgcctg aatatgcaat
    20941 aatatcacct gctttgactc gatcacctac tttgacatta tatttgtcta agtgtagata
    21001 ccactggtga tattctccat tgtcttcggc aatctgtaat accttgccgc ccaattcatt
    21061 attaaagata cgtgttactt taccattcgt cggcgcttta attggtgtac cttttggcaa
    21121 tgcaaaatca atgccataat gcttaccatc aaatggtgaa tgttgatatt taccaaaagt
    21181 ctcagtaatc cgactccctc taaaaaagtc ttcccatttt gagtgtcctt gtcctttatg
    21241 attttgtgtc aatttatcgg tatattggtt aaaccatttt tgaatgtcat ctatataaat
    21301 aatcgtaaat aatagtgaaa gaaaaatgat aattgttgcc aaaatacgtt ttggtcgctt
    21361 tgtcatagtg gcatccatcc ttcatatatt ttgggtatta agtatatatt ctaaatcaac
    21421 tatttcataa ttaaatgcac tcatacttta gtagtactgc caaatataga tgaagaaaat
    21481 gatggtaatt tgaatatttt gattgatatt aaattttata tcgagtataa taattatact
    21541 taagaaaata acagaaacag aggagaattt tcaaatggca aaagtattat atattacagc
    21601 acaccctttt aatgaactgg tatcaaattc tatggcggct ggtaaagcat ttatcgaaac
    21661 atatcaacaa caacatcctg atgatgaagt gaaacatatt gatttatttg aaacttatat
    21721 tccagttatt gacaaagatg tattaactgg ttggggaaaa atgagtaatg gcgaaacgtt
    21781 aactgatgat gaacaaatga aagtttcaag attgagtgac attttagaag aatttttaag
    21841 tgcagataaa tatgtatttg taacgcctat gtggaacctt tctttcccac ctgtagttaa
    21901 agcatatatt gatgctattt caattgcagg taaaactttt aaatattctg ctgaaggacc
    21961 acaaggttta ctaactgaca aaaaagtatt acacattcaa tcacgtggtg gatattatac
    22021 tgaaggacct gctgctgatt ttgaaatggg tgaccgttat ttaagaacaa ttatgacatt
    22081 cttaggtgtg ccatcatatg aaacaatcat tattgaagga cacaatgctg aacctcataa
    22141 gactgaagaa attaaagcaa ctagtattaa taatgctgaa aaattagcaa caacttttta
    22201 aatttttagt tacactgtgt gacgtctcaa acgtcatgca gttttttatt gtatctcttt
    22261 tccgcttttt ataaatatct attattttca taaatttaaa atttgatatt cactttaata
    22321 ctgatgtaaa tatttttcaa aatatattat tatagtgtta atgaagtatt ttgagaaacg
    22381 gtgggaatgt atgaaagcac ataaaatttt ctggttaaac ttagcagcaa ttattattat
    22441 ttcaattgta gtcagtggcg atatgttttt ggcaatgaag tgggaacaaa ttcatttgaa
    22501 agatggtctt aaaaaagtac taagtacgta tcctattaaa aacttagaaa cgctttatga
    22561 aattgatggt cacgataatc cccattatga aaataatgat caagacacat ggtatataga
    22621 atcctcttat tcagttgttg gatcagatga gcttttaaaa gaagaccgta tgttgttaaa
    22681 ggtagataag aacacacata aaataactgg tgaatatgac acaactacaa acgataaaaa
    22741 aaatgctact gactcgacat acaaaagtta tccagtaaaa gtagttaata ataaaatcgt
    22801 tttcacaaaa gacgtaaaag atcccgcgct gaaacaaaaa atcgaaaaca atcaattttt
    22861 gattcaaagt ggtgatttaa caagcatttt aaatagcaat gatttgaaag tcacacatga
    22921 tcctaccact gattattata atttatctgg taagttgtcg aacgataatc caaacgttaa
    22981 acaattaaaa cgtagatata atattcctaa aaacgcatca acaaaggtgg aattaaaggg
    23041 aatgagtgat ttaaaaggca ataatcatca agatcagaaa ctttattttt atttttcaag
    23101 tcctggaaaa gaccaaatca tttataaaga aagccttact tataataaaa taagtgaaca
    23161 ttaatactta tgctgtaatt atagaaacat ccaaatcatc tattaaaatc ctatattata
    23221 aaaacacctc acataactcg ttcaactgta ccaaaccaca ttacattaga ttttaggcta
    23281 actattgtga tgtacatcaa aaacgaattt gtgaggcgtt gtatatttta caaaggtgac
    23341 tagcgtttcg tatagcattt ccaacattac tacactcaag cgtcacgcta aagttcgaaa
    23401 tcgaatcctt tcattcaaca aaagctcata tccactacaa acttcatatc aagcgtataa
    23461 actatcttgt gatactatct cgatcatatc tatagtatgc atttgtgttc cgtttcactg
    23521 aagtatatgt atcatcagtt aagtataaac cgtcatcctt caatgttact tgataagcat
    23581 atttccgtgc taaccaggca atatctatat aattttctcc tgcgttttca taacttctta
    23641 aatcttcaat atgtgcacta acttcaggga aaatgattct aacaacactt tcatcaaccc
    23701 aatatttgtc atgcatccat cgcacttgat ctgccaataa aggtaactgc acatcattga
    23761 aatatagacg aaagccgtca ctatcataca tttgccgata tggtaatggc tgttttctaa
    23821 tcactaacac ctcgccaccc attacggtgc cttctctagt atcatcactt ccacccgaag
    23881 cttcatacgt tgttgggtca acctgtagtc catgtacatc tccaatataa gcatctggtt
    23941 tatgttccat tgcatgtcca tgtgcaatca atgctaatat tgtagattgt gaaaattgag
    24001 gctcccattc aatgcgatta ggatggctac tataaattct aggttcatct atagcctgct
    24061 gaatatccat gccaaacact aatacattga ttaatgtttg cgcaacacta gcaatgatac
    24121 ttatggcacc aggtgcacct actgttaata ttggcttccc gtgatacatc acaatcgttg
    24181 gagccatgtt acttagtggt cgtttatatg gtgcaatttc gttaatacca ccatctacta
    24241 catcaaagcc atccattgtc gtattcaata acacaccgta gcctggaatc gtgatacctg
    24301 aaccataaat cataccaatt gatgtcgtaa atgaagcaat attaccttcc ttatcaatca
    24361 cacaaaaatg agttgtttcg gtatgatttt cttcaacatc tgtatgacta atgcaatcag
    24421 acacaacacc atgctctata tcaatatttg ctttattgct atcaatgagc gtactgcgtg
    24481 cttttaaata atcatcatca attaatgact gtacaggcac ctcatgaaaa ttatcatccg
    24541 ccaagtattg cgcacgatca ctatatgcta aatgcatcgc ttgtatcaaa tgatgcaagt
    24601 aatcaacaga tcttggaccc atagatggta aatcgacatg ttctaataac ttcaatattt
    24661 gaattaccgt gataccgcca gaactagatg gtcccattga ataaatgtca tagtctttaa
    24721 atgttgcact gattggcgct ttaatctgaa tgtcatattt ggctagatcc tctaaagtga
    24781 ttgtcccacc acatgctttg acaacattga ctaattgttt cgcaatgtca cctttataaa
    24841 atgcattaaa cccttgttct cttaatattt gaaatgtctt acctaattcg ggttgtacaa
    24901 tccaatcacc ttcacgccaa tattgatttt catgcgtaaa tacttgtgcc gtttcatgat
    24961 actttgtcaa tcgtgcgtgt tgctggcgcg aatatttttc agtagcccaa ttggctgcat
    25021 gaccttcaat ggctagttca attgcaggat taattaaatc ttccaatgac aatttagcat
    25081 aacgcttgtg aatataatca aacagctttg gaattgctgg cacagcgaca gttttaccat
    25141 gtgtagtcat atcaaaaaat gatttatatt cgcctgaatc atctagataa aattgtttgt
    25201 ctacatgttc aggtgctgtc tcacgtgcat caaacgcagt tatactgcca gtactttgct
    25261 cataatatag caaatacccg ccaccaccaa tacctgatgc aaatggttct aacacattca
    25321 atgccagttg aattgcaatc actgcatcca tggcgttgcc accttgatct aatacatcct
    25381 taccaatttt agccgcaaga ggatgtgata cggaaattaa cccttcttta gatgtttttg
    25441 tctgtttgtc atttaagtta atgaccatac tatatcctcc tactttctgt taaatattta
    25501 aaacattatt gattaatggc tttttctact ttttctaaat cttgacgttg ctcgttacca
    25561 gtatcgacaa gtggtgtaat cggtgatgca attttaaatt tatcgccacg ataaaactta
    25621 ataaattgat cctgatctat cgcattaact actgcttgtc tcaagtttgg atgcgtctta
    25681 aatatacctt ttttaatatt tagcattaaa aagactgact tgcgtccatt tttgcgaata
    25741 atgcttaaat ttttatccga cttaattaaa tcaaaatgtt tttgattcac atctgccaac
    25801 atatcaattg aatgatttct aagttctgac aatgcattat tcgggtcacc attaaacttc
    25861 aatgtaatat ttttaatttt agctggtcca taactacctt tttctgtttc gttgaatcct
    25921 ggattacgtt gaaacgttgc ttgatatgca tttttctgtg tcataatgta tgcgccactt
    25981 gcatacagcg catttttccc atctgaattt gcaggaattg tactgctatc cccatatcct
    26041 tttggatatt cttgatttac ttgattaaca aattttttag ataaaatgcc tgccgaagag
    26101 tgtgttaagt aatttacctc tcgaggcatc gattgatctg tcgtaatttt aacaatttga
    26161 taaataccgt ctttattatt tactttttga ccatctgtcg ttaacgattt gacgttataa
    26221 gctttaatca acttatcata gattgattta tcgtccttgt ctttctcttt acgcaactga
    26281 tcgatgtcct catcttttaa tatcttgatg tcatttatat gtttgtgcat attgtaagta
    26341 ttattgttag gcacagactt tttatcacgt gctctatcta aagaaaactt aacatcttca
    26401 gccgatacac gctctccagt attacgtgct tgtccattga ccactttcgc aaaataatca
    26461 tcatctctta acaagaaata aaatgcttta ttgtccttat tcacagcata atcatgactt
    26521 aacgaacctt tcgttgttaa atgatcattt tcatctaata ataataacct tgtgtacata
    26581 ttcatattaa ttgaatatac tgacggcgca attgaacgta ttggatccaa tgtaggaatt
    26641 tcaccatctt gttgtgtcat cacaagtggc cgcgtatctc gttctctact attgttgtaa
    26701 tcaaattgtt gccatattaa tgcacgtgaa tttggcaatc caacactatt tttatctaac
    26761 actttattgt catatactaa attctttttt gatccatata aaggcgccat atacccttta
    26821 tcaaatacaa cttcatcttc aatttgctta tatgtttgtt taacatctgc ttcattttga
    26881 gtagaagctt tatttaacaa ctggtctaca tgtttatctt tcaataaact atttgatcct
    26941 gtagaactaa ataatgccgt catagcatag ttcgggtcac caaacactgt catccagtca
    27001 tcaatttgga tatcataatt gccggcttga cgttgtgtac gatagctacc ataatctggt
    27061 tggatattca tcttcacgtt aaatcctgca ttttccaatt gatctttaac gatattcata
    27121 tcattttcat aacttgcttg tcctaggaaa tgtattgttg gtcgctcgcc tttcacttca
    27181 actttcgatg acttttgagc cacttctgat ttcgtaggga caccacaacc acttaatacc
    27241 aacgctaaaa ctataattgc gatactaatg attttcttca catctatccc taccttttta
    27301 atgaattctt ggatctagtg catcacgcac tgcatcacct ataaaattaa atgctaaaac
    27361 gacgaacata atacaaacac caggtacaat agctaaatta ctgtgcgttt ccaagtagtt
    27421 actaccggta cgtaaaatgt tgccccattc agctacatca ggtgcaacac caagtcctag
    27481 gaaacttaaa ctacttgttg ttaatacaac cacacctata tttaatgaaa aacgtacaat
    27541 cataggcgca atcgcattcg gtaaaatata acgccatatg atattccaag tgttttcacc
    27601 agtgatacgt gctgcatcta catattccat gcgtttaatt tctaaaacac tggcacgcat
    27661 tgtccgtgca aatgatggta tattaccgat acttaaagca ataattaaat ttggaatact
    27721 tgctccaaat gatgcaataa ttgccaccgc taacaataat gatggaattg caaacactac
    27781 atctaaaatt cgcattatta aattatcaat atgattaaaa taacctgcga tagtgcctag
    27841 taacacacca aaaataactg caataactac tgaaataatt gaaattgaaa atgtcagctt
    27901 cgttcctaca actacgcgtg taaataagtc tctaccgaaa tcatcagtac caaacggata
    27961 ggctagactc ggtccatgta acagtgcatt gaactgattt ttagtagcca atgtcgtatc
    28021 aaatgtaaat tgtgacacaa ttgataatgt cagcatgtag actaaaataa gtaacccgat
    28081 aatcgcaata cgatgtctag tagtttttcg tataaacgat tcccacccgt tataactatg
    28141 tatttgcgat gtacgttggt aacgtctaat acttacaaac attaataatg taaatacgtt
    28201 gcctgttaat gtcatcaaca ataacaacac ttcgacgata cgtcgccata ggtcatgatg
    28261 cttccatgtt tgttccgttg ttaaaataat aattaaaatg atggttaaaa cgattagcaa
    28321 tgtttcagca atatagaacg tatcggccac ataaccttta aaaagattta atgcactcgt
    28381 taatataact aaaatataag ttgctatggc gtaacttgcg aataatttta aggaagctat
    28441 ctttgaatta agttgtgcca tatgcctcac ttcctttcgt tgatttcact acgtaatttt
    28501 ggatcgatta aagcataaaa tatatcaata attaagtttg ctaaagatat tacaattgat
    28561 atatatacga ccccacccat gactgctgga atatcaggta ttagttgttt ttggacgata
    28621 taacgcccga taccattaat gttaaatact tgttccgtca ctgctgaacc gcctagtaac
    28681 tctgccacta gaagaccaac taacgttaca attggaataa tggcattttt caaaatatgt
    28741 ttaataacaa cttgtgtcgt cgataatcct tttgcataag cagttaaaac ataatcgctg
    28801 cgcattactt caagtacaga agaccttgtc atacgcgtga tagaagcagc aatacttgtt
    28861 ccaatgacaa gtacaggtaa aatcaacgat attggatgtt ctggcatata agatggtggc
    28921 aaaatatcca atttcaatga gaacgctaaa atgaataata gcccttgcca gaaacttgga
    28981 atagataaac caattaatgc aattatcatt aacgtgatat caagccaact atttcgcttc
    29041 atcgcactga taataccaat tggtattgca ataattaatg ccaccattag cgctaatact
    29101 gcgacaatta ttgtaattgg aattctttcg ccaactgctt tagtcacaac ctcattccct
    29161 ttgtaagtcg tacctaagtc aaaggtaaaa acacccttga tggtatccca caattgaata
    29221 aaataaggtt cgttaagatg atgtaataca ttgaattgat gtatctgtgc ctttgttgca
    29281 ttttgtccca gtatgctata agccgcatca agcggtgaaa aatacagaat ggtaaacaca
    29341 ctgacaataa caccaatgat gacaatcaca gccatgacaa ttcgttcaaa aatatatcta
    29401 actaatggct gtaaataaaa agtcaataag atgaacatcg gcaaggccaa tatcactttg
    29461 atcatgatga acttatgaaa taatacattt tcaaagtatg ttgaaaaatg tgcttgttca
    29521 atattctttg aactcgtatt agaactttgt gccttgaata tttttaatgc ttctttatgt
    29581 atttgtgtgg atgacttttg ctgcgataaa tatttatatt tttgatgtaa cgcctgttca
    29641 atttctgaaa tttcagaatt attagcgtaa aaatttttcc tcttagcaga aaagaaaaac
    29701 tttatcactg catataaaaa tattggcaag cttaataccg ataatacaaa cttgtaaaat
    29761 gccttgtgat acaacgactt aaaagattct aaataagtaa ttatgataat cactccttgt
    29821 aaaataatct tgagtagatt actatgatat acaaaagtat agaataaatt tacacatttg
    29881 tgaataggga ggcacaacat catgtcaaat ttattagaag tcaacagtct gaatgtacaa
    29941 ttcaattatg atgaaactac agttcaagcg gtaaaaaacg tctctttcga attacgaaaa
    30001 aaacatatcc taggtattgt tggtgaatca ggatcaggaa aaagtattac cgctaaatct
    30061 attttagggc tactaccaga ttatccagat cacacattaa caggagaaat tatttttaat
    30121 gggcaatcgt taaataattt atcaacttca gcgttacaac aaattcgagg taaggatatt
    30181 tcaatgattt ttcaagatcc actctcttcg ttgaatccaa gattaacgat tggcaaacaa
    30241 attacagaag taatatttca acataaacgt gtatctaaat ctgaagcaaa gtcgatgaca
    30301 atagacattt tagaaaaagt aggtataaaa catgcaactc gacaatttga tgcttatcca
    30361 catgaacttt ctggtggtat gcgtcaacgt gtcatgatag caatggcatt gattttaaag
    30421 ccacaaattt taatcgcaga tgaaccaaca acggcattag atgccagtac acaaaatcaa
    30481 ttactgcagt taatgaagtc cctttatgag tacacagaaa catctattat ttttatcact
    30541 cacgatttag gcgctgtgta tcaattttgc gacgatgtga ttgtaatgaa agatggaagt
    30601 gtcgttgaaa gtggcacggt tgaaagtatt tttaaatcgc cacaacatac ctatacaaaa
    30661 cgcttaatag atgcgattcc tgatattcat caaacgcgtc cgccaagacc gttaaacaat
    30721 gatattttat taaaattcga tcgcgtgagc gtggattaca catcaccgag tggcagccta
    30781 taccgagcag ttaatgatat taacttggct attagaaaag gcgaaacatt aggcattgtc
    30841 ggtgaatcag ggtcagggaa atcgacatta gctaagacgg tcgtcggtct aaaggaagtg
    30901 tcagaaggct ttatttggta taacgaatta ccattaagtt tatttaaaga tgatgaattg
    30961 aaatctttac gacaagagat acaaatgatt tttcaagatc cattcgcatc tattaatcca
    31021 agatttaaag tcattgatgt gattaaacga ccactaatca ttcatgggaa agtcaaagat
    31081 aatgatgaca ttattaaaac tgtcgtatcg ttgttagaaa aggttggcct agatcaaact
    31141 ttcttatatc gctatccaca cgaattatct ggtgggcaac gtcagcgtgt aagtatcgcg
    31201 agagcacttg ctgttgaacc taaagtgatt gtttgcgacg aggcagtgtc cgctttagac
    31261 gtttcaattc aaaaagatat catcgagtta ttaaaacaat tacagttaga cttcggcatc
    31321 acttatttat tcatcacaca tgacatgggt gttatcaatg aaatatgtga tcgcgttgca
    31381 gttatgaaaa atggcgaaat cgttgaactg aataacacag aagatattat caaacatccg
    31441 cagtcagact atgcaaagca acttatttca gaagtagcag ttattgctaa ataaaagtca
    31501 tgcgttgtgc aactttatca ctgtatggtc tgaaataaat tgcgcgactt ctgatgtgtt
    31561 atgttagatg actttgtcat actaactata ttgtgctaag ttagggctcg gcaccatatc
    31621 aagttttagg tgctttgcca tgatttaaga gtcaccccca tactttgggc attttaacgc
    31681 cagaataaat cccccgccac tatgtgaagt gcgggggatt atttatattt tattagaata
    31741 ttcagatttt tgagtgtgtc aacttagctt agtcaatgta tatttaacgt cacttactct
    31801 ttttctttca taattaacac attcaaataa actttgatca aaaaacacaa agttaaaagt
    31861 accatcttgt aatatgctct catacattat cccgtcatat ttaaggcttc gaatataatc
    31921 agctaaatat tgaaatggca aataatctat tccttgttca tcgcttggat ttgttattcc
    31981 tttatgaatc ttttttaatg tttggtaatt tacaaaatac tttctaaatc catcatcgcc
    32041 agctttgatt gcattactag ttaaattagt taaattcgca attttcaatt tctcttttgt
    32101 cacgtttttt tgtaacttaa ccttacctat ataaataatg tcattatgct taggtttaac
    32161 ttcttctata ctgacctgtt cttttgtact aaggtataat acgcttatcc atttagaatt
    32221 caatcttcct gccgttgcaa atccctttgg tggtgacatt agttcacttt tctctgtaat
    32281 gaacttaact attctagatc tatataatgg ttcaaatctt tctctaaatt cctcaatact
    32341 atagtaatta gtagtgatat cgagaaagaa cgctaaattc tctaaattga tcatattatt
    32401 atgaaatcta tttttatact tcaagctctc acaaaatcca tcccagtcat tatttgctac
    32461 aattagattt ttatttgtat attttttatc gtttatgatt ttagcgccta ctaaatcttc
    32521 caacactcgt ctatctaaat tttcatcatc tttaaaaagt tcatttaaaa tacaacttat
    32581 ttgagcttcc tcaacattaa atatactcca gtcgtctttt aatgctattt caatcttttt
    32641 acc
//
LOCUS       DAKTIO010000011        32606 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.011, whole genome shotgun sequence.
ACCESSION   DAKTIO010000011 DAKTIO010000000
VERSION     DAKTIO010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 32606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32606
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.011"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(74..1954)
                     /gene="vraE"
                     /locus_tag="O0293_000383"
     CDS             complement(74..1954)
                     /gene="vraE"
                     /locus_tag="O0293_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter permease
                     subunit VraE"
                     /protein_id="HCY0279293.1"
                     /translation="MTFNHIVFKNLRQNLKHYAMYLFSLFFSIVLYFSFTTLQFTKGV
                     NNDDSMAIIKKGALVGSIFLFIIIVIFLMYANHLFVKRRTREFALFQLIGLTRQNILK
                     MLALEQMIVFLITGVVGVLCGIAGAQLLLSIVSKLMSLSINLSIHFEPMALVLTIFML
                     IIAYVLILFKSALFLKRRSILSMMKDSIKTDATTAKVTTAEVISGVLGIAMIALGYYM
                     ATEMFGTFKALTMAMTSPFIILFLTVVGAYLFFRSSVSLIFKTLKKSKNGRVSITDVV
                     FTSSIMYRMKKNAMSLTIIAIISAVTVTVLCFAALSKSNTDQTLTSMAPNEFNVVATQ
                     DAKQFETKLSQQQITFSKNAYETITVDNVNDQVITLENGSDSGRTNSILSANNKLTGN
                     NAIITNTKSLPNIINIHLNKDLVVKGTKNETFRVTQEDKGKVYPLNLSFNSPVIEVSP
                     EKYQQLKTQNNVHTFYGYDIKQTSQKEKAQAIAKQFGDKVITYDEMKKEVDATNGILI
                     FVTSFLGLAFLVAAGCIIYIKQMDETEDELSNFRILKRIGFTHTDMLKGLLLKITFNF
                     GLPLLIAILHAVFAAIAFMKLMGNISFMPVIVVIVVYTLIYITFALIAFVHSNKLIKK
                     TI"
     gene            complement(1944..2702)
                     /gene="vraD"
                     /locus_tag="O0293_000384"
     CDS             complement(1944..2702)
                     /gene="vraD"
                     /locus_tag="O0293_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter ATP-binding
                     subunit VraD"
                     /protein_id="HCY0279294.1"
                     /translation="MTILSVQHVSKTYGKKHTFQALKDINFDIQKGEFVAIMGPSGSG
                     KTTLLNVLSSIDQISSGSVIANGQELNKLNQKALAKFRKESLGFIFQDYSILPTLTVK
                     ENIMLPLSVQKMSKATMEENYKAITTALGIYDLGNKYPSELSGGQQQRTAAARAFVHK
                     PQIIFADEPTGALDSKSANDLLQRLEEMNKSFDTTIVMVTHDPVAASFAERVIMLKDG
                     QIHTQLYQEGRSKQAFYEDIVHLQSVLGGVSNDI"
     gene            2985..3497
                     /locus_tag="O0293_000385"
     CDS             2985..3497
                     /locus_tag="O0293_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HCY0279295.1"
                     /translation="MEKGNQGIKWSSLIMGVLLLMLAVVIFTFPIENFYAITWLIGLF
                     VLINGVIQIVYRRKAKALVGGNQNWILFMGIVDILFGLLVIFNVGASSAFFIYMFAFW
                     FIFSSISGLFTFSGSGSLKLISVIFNLLGIVFGVILLFNPLMGIVFISTMIAIAFVFV
                     GVIYVVDALA"
     gene            complement(3631..4425)
                     /locus_tag="O0293_000386"
     CDS             complement(3631..4425)
                     /locus_tag="O0293_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HCY0279296.1"
                     /translation="MNIAKLENYLQIDSSRYNRPSIEALNYYATRFMLTVPFENIDVQ
                     NSKPISINIDALFNKIVHDKRGGFCYELNTFFKAYLQQKGFNPELMSATIHTPGGGRS
                     LNGSHASLVVSINDVFYVTDVGFGDLPLHAIPITSSEHTQPITDISGTFRAIFNNEDK
                     DIFYVQKFENDHWHTKYEAEFKPKQIEDFNSNIEYNQTHPDSIFVQHLLITMPQSFGR
                     ATMSENHLTLTRNGSSEKFDVTKDNYKHFLEKYFGLNVTINRIEKQ"
     gene            complement(4668..5684)
                     /locus_tag="O0293_000387"
     CDS             complement(4668..5684)
                     /locus_tag="O0293_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HCY0279297.1"
                     /translation="MTVFKNERLSWLPYIAIVILLHVIGFSFLWIAGKDHHILFGMGI
                     LAYTLGLRHAFDADHIAAIDNTVRKLLQQRKDPSGVGFYFSIGHSSVVFLMAVFLGVS
                     VKWAKDELPHFQDIGGTIGTLVSGFFLVLIGVLNLIILISLINLFAKLRREHIEEAEV
                     DALLESRGLVSRFVGPYFKLITRSWHVLPLGFLFGLGFDTASEIALLALSSGASQQAI
                     SFIGILSLPILFASGMSLLDTLDGVVMKYAYNWAFFNPIRKIYYNITITAISVMAALV
                     IGMIELLQILADKLDLHGAFWAFIGSIEFDYLGYILVALFLITWLISSLIWKFGRIEH
                     KWSR"
     gene            complement(5849..6424)
                     /locus_tag="O0293_000388"
     CDS             complement(5849..6424)
                     /locus_tag="O0293_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCY0279298.1"
                     /translation="MTLPKIGKPATRALNSQGIYTLEAVSQYTKSSLMEMHGVGPKAI
                     SILEQALFQHQLHFKTEVQSSLPFKLTGDVSCNHAPKRQQMIDFIVVTAALDIELLRS
                     LVTTEFIWSVPGRFDIYGPQILIQELSNHYNQVASLNIHSSITHGCLGSMHGIEILKT
                     GKEIHFAHFFEFENHKKDAKLSKVTSYIVVG"
     gene            6649..7557
                     /gene="rarD"
                     /locus_tag="O0293_000389"
     CDS             6649..7557
                     /gene="rarD"
                     /locus_tag="O0293_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCY0279299.1"
                     /translation="MNSEYKKGIFLALSAYILWGILPIYWQFVDAIGAFEILAFRIIF
                     SAIFMIFILAVGQKQRNAFQRDMNQLLGKPIQLLAIVVAGYVITLNWGTFIWAVTNGH
                     VLQTSLGYYINPLVSILLALIFLKERFNKFEWLAILFAFIGVLYMTLKIGEFPIVSII
                     LALSFGTYGLLKKVVHIDAISSITIECIVTAPAGLIYVIYLWQQHQMSFGLNMSSFWL
                     LFSGAITAIPLILFSAGAKRIPLSLIGFIQYVGPTIMFVLGIFVFKEPFSIDQLITFI
                     FIWTGIVLYSLSQYIKLKKHPVAKTL"
     gene            complement(7970..9388)
                     /locus_tag="O0293_000390"
     CDS             complement(7970..9388)
                     /locus_tag="O0293_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCY0279300.1"
                     /translation="MENTVKYRKFILPIVVGLLIWALTPFKPDAVDPTAWYMFAIFVA
                     TIIACITQPMPIGAVSIIGFTIMVLVGIVDMKTAVAGFGNNSIWLIAMAFFISRGFVK
                     TGLGRRIALHFVKLFGKKTLGLAYSIVGVDLILAPATPSNTARAGGIMFPIIKSLSES
                     FGSKPKDGSARKMGAFLVFTEFQGNLITAAMFLTAMAGNPLAQNLASSTSNVHITWMN
                     WFLAALVPGLVSLIVVPFIIYKIYPPTVKETPNAKSWAENELATMGKIALAEKFMIGI
                     FVVALTLWIVGSFIHIDATLTAFIALALLLLTGVLTWQDILNETGAWNTLVWFSVLVL
                     MADQLNKLGFIPWLSKSIATSLGGLSWPIVLVILILFYFYSHYLFASSTAHISAMYAA
                     LLGVAIAAGAPPLFSALMLGFFGNLLASTTHYSSGPAPILFSSGYVTQKRWWTMNLIL
                     GFVYFIIWIGLGSLWMKVIGIF"
     gene            complement(9626..10090)
                     /gene="bstA"
                     /locus_tag="O0293_000391"
     CDS             complement(9626..10090)
                     /gene="bstA"
                     /locus_tag="O0293_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol transferase BstA"
                     /protein_id="HCY0279301.1"
                     /translation="MTTKTVFDVIDMGLGYLVNVYDAWKVEKVLDDYHKPFSNTIHWQ
                     FGHVLTIFESALAVAGKENIDLNIYRPLFGNGSSPDEWKDEVPSIERILEGLQTLPER
                     ARNLTEDDLAIELKQPIVGCNNLEELLVLNAIHIPLHAGKIEEMSRILKNLK"
     gene            10257..10595
                     /locus_tag="O0293_000392"
     CDS             10257..10595
                     /locus_tag="O0293_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCY0279302.1"
                     /translation="MSVSDQIMKGLLDGAILGLIAQGETYGYEIMTKLKNQEFPEISE
                     GSIYPVLMRLNKKGYVDTTIKKSSNGGPRRKYYTITDSGLKELKVFKSKWQVLNKGMI
                     NLFGGENDVK"
     gene            10585..11352
                     /locus_tag="O0293_000393"
     CDS             10585..11352
                     /locus_tag="O0293_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279303.1"
                     /translation="MLSKQSQDFLEKLRVELLFRGKTEAEVEELLEELEDHLTMAEKN
                     NEDTSSIINTPIKSMADQLSPEISLTTGLYKYITLYFAFILAVIIIPRFLDLGTFDVT
                     IAFLLYIASIVILGVVIGMLILRNTLVRFGDQKITYVINVTYGIIVFLWMILGGLIIK
                     KYPIYSFFELSDKQSFIVGLVLLIALIGTCILIKQKIYALIVLVISLPSLIARIAAIF
                     SKEPDTFNLVSVSILIILNIAFIIYTFVNFRKLKKED"
     gene            complement(11471..12109)
                     /gene="pcp"
                     /locus_tag="O0293_000394"
     CDS             complement(11471..12109)
                     /gene="pcp"
                     /locus_tag="O0293_000394"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HCY0279304.1"
                     /translation="MHILVTGFAPFDNQNINPSWEAVTQLEDIIGTHTIDKLKLPTSF
                     KKVDNIINKTLASNHYDVVLAIGQAGGRNAITPERVAINIDDARIPDNDDFQPIDQAI
                     HLDGAPAYFSNLPVKAMTQSIINQGLPGALSNSAGTFVCNHTLYHLGYLQDKHYPHLR
                     FGFIHVPYIPEQVIGKPDTPSMPLEKIVAGLTAAIEAISNDEDLHLALGTTE"
     gene            complement(12261..13217)
                     /locus_tag="O0293_000395"
     CDS             complement(12261..13217)
                     /locus_tag="O0293_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCY0279305.1"
                     /translation="MNYQVLLYYKYMTIDDPEQFAQDHLAFCKAHHLKGRILVSTEGI
                     NGTLSGTKEETEQYMAHMHADERFKDMVFKIDEAEGHAFKKMHVRPRKEIVALDLEDD
                     VDPRHTTGQYLSPVEFRKALEDDDTVIIDARNDYEFDLGHFRGAIRPNITRFRDLPDW
                     IKENKALFADKKVVTYCTGGIRCEKFSGWLLKEGFEDVAQLHGGIATYGKDPETKGEY
                     WDGKMYVFDDRISVDINQVEKTIIGKDWFDGKPCERYINCANPECNKQILVSEENETK
                     YLGACSYECAKHERNRYVQANNISDNEWQQRLTNFDDLHQHA"
     gene            13461..14432
                     /locus_tag="O0293_000396"
     CDS             13461..14432
                     /locus_tag="O0293_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HCY0279306.1"
                     /translation="MSQQDLPTLFYSGKSNSAVPIISESELQTITAEPWLEISKKGLQ
                     LEGLNFDRQGQLFLLDVFEGNIFKINPETKEIKRPFVSHKANPAAIKIHKDGRLFVCY
                     LGDFKSTGGIFAATENGDNLQDIIEDLSTAYCIDDMVFDSKGGFYFTDFRGYSTNPLG
                     GVYYVSPDFRTVTPIIQNISVANGIALSTDEKVLWVTETTANRLHRIALEDDGVTIQP
                     FGATIPYYFTGHEGPDSCCIDSDDNLYVAMYGQGRVLVFNKRGYPIGQILIPGRDEGH
                     MLRSTHPQFIPGTNQLIICSNDIEMGGGSMLYTVNGFAKGHQSFQFQ"
     gene            complement(14581..15096)
                     /locus_tag="O0293_000397"
     CDS             complement(14581..15096)
                     /locus_tag="O0293_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HCY0279307.1"
                     /translation="MTNFTFDGAHSSLEFQIKHLMVSKVKGSFDQFDVAVEGDINDFS
                     TLKATATIIPSSINTKNEARDNHLKSGDFFGTDEFDKITFVTKSVSESKVVGDLTIKG
                     ITNEETFDVEFNGVSKNPMDGSQVTGIIVTGTINREKYGINFNQALETGGVMLGKDVK
                     FEASAEFSISE"
     gene            15476..16324
                     /locus_tag="O0293_000398"
     CDS             15476..16324
                     /locus_tag="O0293_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HCY0279308.1"
                     /translation="MGNYLVLQSDFGLSDGAVSAMYGVAYTVSDDIRVENLTHDIPPY
                     DIWVASYRLYQTVKYWPKGTVFVSVVDPGVGSDRRSIACLTYSGHYIITPDNGSLSHI
                     KHYEGIKKVIEIDEVKSRLPHSEESHTFHGRDVYAYNGARLAANKIAFERLGQAINVD
                     SIEALEIREVTKNEDSVTGSIDILDIRFGSLWTNIPLAFLKSLEIVHGNNLVVTIYHQ
                     DKKVYQNIIKFARSFADVNVGEPLVYVNSLVNIGVAVNQDSFSDLYHIGTGNDWTIHL
                     SKAPSI"
     gene            16353..16907
                     /locus_tag="O0293_000399"
     CDS             16353..16907
                     /locus_tag="O0293_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HCY0279309.1"
                     /translation="MKKQDISVKTVVAIGIGAAVFVILGRFVVIPTGFPNTNIETSYA
                     FLALISAIFGPFAGLMTGLVGHAIKDFTTYGSAWWSWVICSGIIGCLYGWIGLKLNLS
                     SGRFSRKSMVYFNIGQIIANIICWALIAPTLDILIYNEPANKVYTQGVISAVLNIISV
                     GIIGTILLKAYASSQIKKGSLRKE"
     gene            16960..18672
                     /locus_tag="O0293_000400"
     CDS             16960..18672
                     /locus_tag="O0293_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0279310.1"
                     /translation="MTEPIISFKDFSFQYHSQATPTLQNINVDIYPGEKVLVVGASGS
                     GKSTFANCINGLIPFKTKGNITGELYINNQDATVSCLHDRSNVVGTVLKDTDGQFIGL
                     TAAEDMAFLLENNCVEQDDMKKNVSYWAEKVGMIEHLNHRPQDLSGGQKQRVSLGGIL
                     IHRTPILILDEPLANLDPATGHETLRLLNNIHEETKSTMIIVEHRLEESLDDTFDRVL
                     LFKDGKIIANTTPSDLLKSSKLKEAGIREPLYCTALKYAEVDVESIDNLANLRDVCMS
                     EYVKFKVKKWIDETSANNDNKYKSEPLLELNEVCVQYSDYSNSVLNNVQLNVYRREML
                     SIVGHNGAGKSTLAKAICGFLDITGNIQFCNRGFNQLSISERSEFVGYVMQNPNHMIS
                     EKMIYDEVALGLRARGMKESDIKIRVENVLKICGLYAFRNWPIAALSYGQKKRVTIAS
                     VLVLNPEIIILDEPTAGQDFYHYNEIMSFLIELNRQGKTIIMITHDMHLLSEYSSRTV
                     VLSKGQVVADTTPVLVLNDKKICEIASLRQTSLFEMAEYIGISEPQKLVQLFINHDRK
                     VRRQ"
     gene            18669..19502
                     /locus_tag="O0293_000401"
     CDS             18669..19502
                     /locus_tag="O0293_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCY0279311.1"
                     /translation="MNQYNTIGFHPGNSRIHQLNATVKLLFLLVVSISAMVTYDTRYL
                     ILISASSILLVKYAHIEWKQVRFVVKFILFFTILNIIAVYIFDPEYGVKIYNQRTELV
                     NGIGRFTLTSQELFYLFNLILKYISTVPLALIFLFTTNPSHFAASLNQLGVNYKISYA
                     VSLALRYIPDIQETYFNISQAQQARGYDNSKKAKFTSRVKGIKRIVLPLIFSSIERID
                     TISTAMELRQFGQYKRRTWYVKKQLKKDDYVVLCLTLILLMLVVTLFFLNNSRYFNPW
                     H"
     gene            19549..20046
                     /locus_tag="O0293_000402"
     CDS             19549..20046
                     /locus_tag="O0293_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0279312.1"
                     /translation="MINTERLNLMIPSSSHLIELYNICSHPQANIYTPKGLHNSKLDT
                     QRWIEKWRNHWQQYQFGYFVLVKKIDCSVIGICGYEYRQLKQETVLNLFYKLHPSFEG
                     QGYACEAITAITNFVNYIDQETVKVIRTNKCNQRSINLAERLKFKRDDTMDDIINQGD
                     IVFYK"
     gene            20164..21288
                     /locus_tag="O0293_000403"
     CDS             20164..21288
                     /locus_tag="O0293_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCY0279313.1"
                     /translation="MHKKYLLLFFLLLSVALLYSCSKQNEKAQNKSVNPNEYAKHKSK
                     MDEHWETYHGQVYHVFYHPLITDPKVAFTGDTHQAKGNNDWMITVSEFKKSLNELYKN
                     NYIIVNPHDVYDLSSHHVKAKKIKLPKGKKPLIISIDDMNYYSYMRHHGYADRLVLDK
                     NKHVVSETTDKKGHATYSDDNDIVPILNKFVKEHPDFSLNGEKGVVALTGYEGVLGYR
                     TNELTSKDYTKNKKAAEEVVRAMKRDGWSFASHSYGHINFEKTSLEGIKRDTKRWKDE
                     VEPIVGKTDMFVFPHGAQDRHTQAYDYLVDEAEFKFIAGVGPNNFTDISATNVYQDRV
                     AIDGLNLFEFKYKLKPFFNPENVYSKQDRRYFKGNRDYEE"
     gene            21593..22411
                     /locus_tag="O0293_000404"
     CDS             21593..22411
                     /locus_tag="O0293_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HCY0279314.1"
                     /translation="MNNSEQLIALKESETAFLKYFNKADYELVDFSVVEKLDWKQLNH
                     EDLQQMGERNFWQHEHQIYALRNDFTDQLLRYYSMYPTAATKVAYTGLIIRNNEAAVQ
                     VGLENYAPSLANVQQSLKLFIQFIQQQLRDNVHFVVLGHYQLLDALLDKSLQTPDILS
                     MIEERNLSGLVTYLSTEHPIVQILKENTQQQLNVLEHYIPNDHPALVELKIWERWLHK
                     QGYKDIHLDITAQPPRSYYTGLFIQCHFAENESRVLTGGYYKGSIEGFGLGLTL"
     gene            22429..23043
                     /locus_tag="O0293_000405"
     CDS             22429..23043
                     /locus_tag="O0293_000405"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCY0279315.1"
                     /translation="MLRIAIAKGRLMDSLINYLDVIEYTTLSETLKNRERQLLLSVDN
                     IECILVKGSDVPIYVEQGMADIGIVGSDILDERQYNVNNLLNMPFGACHFAVAAKPET
                     TNYRKIATSYVHTAETYFKSKGIDVELIKLNGSVELAGVVDMVDGIVDIVQTGTTLKA
                     NGLVEKQHISDINARLITNKAAYFKKSQLIEQFIRSLEVSIANA"
     gene            23036..24286
                     /locus_tag="O0293_000406"
     CDS             23036..24286
                     /locus_tag="O0293_000406"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCY0279316.1"
                     /translation="MLNAQQFLNQFSLEAPLDESLYPIIRDICQEVKVHGDKALKMYN
                     LTFDHTKTDHLEISHEQIKAAFDTLDEKTKQALQQSYERIKAYQESIKQTNQQLEESV
                     ECYEIYHPLESVGIYVPGGKASYPSTVLMTATLAQVAGVENIVVVTPPQPNGVSQEVL
                     AACYITQVDQVFQVGGAQSIAALTYGTETIPKVDKIVGPGNQFVAYAKKYLFGQVGID
                     QIAGPTEIALIIDETADLDAIVYDVFAQAEHDELARTYAISEDAQVLKDLESRIAKAL
                     PNVDRYDIVSKSIANQHYLIHASNFDEACHVMNTIAPEHASIQTVNPQPYIEKVKYVG
                     ALFIGHYSPEVIGDYVAGPSHVLPTNRTARFTNGLSVNDFLTRNTVIHLSKDTFEQIA
                     DSAQHIAHVEALYNHQQSILIRQS"
     gene            24302..25315
                     /locus_tag="O0293_000407"
     CDS             24302..25315
                     /locus_tag="O0293_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HCY0279317.1"
                     /translation="MIYIDKNESPVTPLDEKTMTSIISATPYNLYPDAAYEQFKEAYA
                     KFYGLSPEQIIAGNGSDELIQKLMLIMPEGPALTLNPDFFMYQAYAAQVNREIAFVDA
                     GSDLTFDLETILTKIDEVQPSFFIMSNPHNPSGKQFDTAFLTAIADKMKALNGYFVID
                     EAYLDYGTAYDVELAPHILRMRTLSKAFGIAGLRLGVLISTAGTIKHIQKIEHPYPLN
                     VFTLNIATYIFRHREETRQFLTMQRQLAEQLKQIFDTHVADKMSVFPSNANFVLTKGS
                     AAQQLGQYVYEQGFKPRFYDEPVMKGYVRYSIATASQLKQLEEIVKEWSAKYDLSKTT
                     KHS"
     gene            25284..25862
                     /gene="hisB"
                     /locus_tag="O0293_000408"
     CDS             25284..25862
                     /gene="hisB"
                     /locus_tag="O0293_000408"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HCY0279318.1"
                     /translation="MIYQKQRNTAETQLNISISDDQSPSHINTGVGFLNHMLTLFTFH
                     SGLSLNIEAQGDIDVDDHHVTEDIGIVIGQLLLEMIKDKKHFVRYGTMYIPMDETLAR
                     VVVDISGRPYLSFNASLSKEKVGTFDTELVEEFFRAVVINARLTTHIDLIRGGNTHHE
                     IEAIFKAFSRALGIALTATDDQRVPSSKGVIE"
     gene            25859..26437
                     /gene="hisH"
                     /locus_tag="O0293_000409"
     CDS             25859..26437
                     /gene="hisH"
                     /locus_tag="O0293_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCY0279319.1"
                     /translation="MIVIVDYGLGNISNVKRAIEHLGYEVVVSNTSKIIDQAETIILP
                     GVGHFKDAMSEIKRLNLNAILAKNTDKKMIGICLGMQLMYEHSDEGDASGLGFIPGNI
                     SRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGADIPAIVQF
                     NNYIGIQFHPEKSGTYGLQILRQAIQGGFIND"
     gene            26430..27134
                     /locus_tag="O0293_000410"
     CDS             26430..27134
                     /locus_tag="O0293_000410"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCY0279320.1"
                     /translation="MIELWPAIDLIGSTSVRLTEGKYDSEEKMSRSAEESIAYYSQFE
                     CVNRIHIVDLIGAKAQHAREFDYIKSLRRLTTKDIEVGGGIRTKSQIMDYFAAGINYC
                     IVGTKGIQDTDWLKEMAHTFPGRIYLSVDAYGEDIKVNGWEEDTELNLFSFVRRLSDI
                     PLGGIIYTDIAKDGKMSGPNFELTGQLVKATTIPVIASGGIRHQQDIQRLASLNVHAA
                     IIGKAAHQASFWEGLK"
     gene            27131..27889
                     /gene="hisF"
                     /locus_tag="O0293_000411"
     CDS             27131..27889
                     /gene="hisF"
                     /locus_tag="O0293_000411"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCY0279321.1"
                     /translation="MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAMYYNEAGA
                     DELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVGGGIQSLDDITQLLNHGADKVSL
                     NSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTTGGKKMTNIKVYDWVQ
                     QVEQLGAGELLVTSMGHDGMKQGFDIEHLANIKSLVNIPIIASGGGGNAQHFVELFDQ
                     TDVSAGLAASILHDRETTVQSIKEVIRQGGIAVR"
     gene            27886..28518
                     /locus_tag="O0293_000412"
     CDS             27886..28518
                     /locus_tag="O0293_000412"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCY0279322.1"
                     /translation="MTKYKIDFSKGLVPAILQDNQTKQVLMLGYMNQEAFDKTIEDGV
                     VCFYSRSKQRLWTKGETSGHTQRVKDIHVDCDNDTILIDVIPNGPTCHTGSQSCFNTE
                     VPFSVQTLAQTVQDSAQSNNEKSYTKYLLTEGIEKITKKYGEEAFEVVIEAIKGDKKA
                     FVSEVADELYHLFVLMHALGVDFSEIEAELARRHHKRNNFKGERQNIEQW"
     gene            28711..28890
                     /locus_tag="O0293_000413"
     CDS             28711..28890
                     /locus_tag="O0293_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279323.1"
                     /translation="MKSKLSNSGIDVTVKDVEKYMNRYNEVMKGKNGEKAKELCLSLL
                     PINIIVVFTFFVFIL"
     gene            29611..31653
                     /gene="lip1"
                     /locus_tag="O0293_000414"
     CDS             29611..31653
                     /gene="lip1"
                     /locus_tag="O0293_000414"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip1"
                     /protein_id="HCY0279324.1"
                     /translation="MKSQNKYSIRKFSVGASSILIATLLFLSGGQAQAAEKQVNMGNS
                     QEDTVTAQSIGDQQTRENANYQRENGVDEQQHTENLTKNLHNDKTISEENHRKTDDLN
                     KDQLKDDKKSSLNNKNIQRDTTKNNNANPSDVNQGLEQAINDGKQSKVASQQQSKEAD
                     NSQDSNANNNLPSQSRIKEAPSLNKLDQTSQREIVNETEIEKVQPQQNNQANDKITNY
                     NFNNEQEVKPQKDEKTLSVSDLKNNQKSPVEPTKDNDKKNGLNLLKSSAVATLPNKGT
                     KELTAKAKDDQTNKVAKQGQYKNQDPIVLVHGFNGFTDDINPSVLAHYWGGNKMNIRQ
                     DLEENGYKAYEASISAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYEGI
                     YKDWKPGQKVHLVGHSMGGQTIRQLEELLRNGNREEIEYQKKHGGEISPLFKGNHDNM
                     ISSITTLGTPHNGTHASDLAGNEALVRQIVFDIGKMFGNKNSRVDFGLAQWGLKQKPN
                     ESYIDYVKRVKQSNLWKSKDNGFYDLTREGATDLNRKTSLNPNIVYKTYTGEATHKAL
                     NSDRQKADLNMFFPFVITGNLIGKATEKEWRENDGLVSVISSQHPFNQAYTKATDKIQ
                     KGIWQVTPTKHDWDHVDFVGQDSSDTVRTREELQDFWHHLADDLVKTEKLTDTKQA"
     gene            complement(32100..>32606)
                     /gene="icaC"
                     /locus_tag="O0293_000415"
     CDS             complement(32100..>32606)
                     /gene="icaC"
                     /locus_tag="O0293_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide intercellular adhesin
                     biosynthesis/export protein IcaC"
                     /protein_id="HCY0279325.1"
                     /translation="SENTIIFGWIFYFFLGAYMGYNYERVLNFLERYLVIMIVLAVAT
                     YFVFIALANGDYWNVTSFSYSLTPYNSIMFIVILGICTHFKTMLFNTIQMISAFSFFI
                     YLLHPIILDSLFAYTNIFEDNTMVFLAISLLFILGLCIGVGMILREFYIFRFIIGKQP
                     YKLNINAY"
BASE COUNT        11476 a         4933 c         5891 g        10306 t
ORIGIN      
        1 ttaaatcaat taatagttat actgcattgc ttaaaatcaa aatatctaat gaagcatctt
       61 ttaatcgtaa gtgttaaatg gttttcttaa tcaatttgtt tgaatgtaca aaagcaatta
      121 aagcaaaagt aatgtaaatg agggtgtata caacaattac tacaataacc ggcatgaagg
      181 aaatattacc catcaatttc ataaatgcta ttgcggcgaa tactgcatgt aatatagcaa
      241 tcaataatgg taaaccgaag ttgaaagtaa tttttaataa taatccctta agcatatctg
      301 tatgcgtaaa gcctatgcgt tttaatattc tgaagttact tagttcatcc tcagtttcat
      361 ccatttgttt aatataaata atacatccag ctgctactaa aaatgctaat cctaaaaatg
      421 atgtaacaaa tattagaata ccgttagtag catcgacctc ttttttcatt tcatcatacg
      481 tgatgacttt gtctccaaac tgttttgcaa ttgcttgagc tttttccttt tgtgatgttt
      541 gtttaatatc atatccataa aaagtatgaa cgttattttg tgttttcaac tgctgatact
      601 tttcaggact tacttcgatg acaggtgagt tgaagcttag atttaaagga taaaccttac
      661 ctttgtcttc ttgtgtaaca cggaaagttt cattcttagt accttttact actaaatctt
      721 tgtttaaatg gatattaata atgttaggca gcgattttgt atttgtaatg atggcattgt
      781 tgcctgttaa cttgttattt gcacttaaaa tagaattcgt gcgacctgaa tcactaccat
      841 tttccaaagt aataacctga tcattaacat tatctacagt aatagtttcg taagcatttt
      901 tagaaaatgt aatttgttgt tggcttagtt tagtttcaaa ttgtttagca tcttgagtag
      961 cgaccacgtt aaattcattt ggtgccatag atgtaagggt ttgatctgta tttgatttag
     1021 ataatgccgc aaaacacaat acagttactg taactgcaga aataattgca atgatagtta
     1081 aagacatggc atttttcttc attctgtaca taatagacga tgtgaataca acatcggtaa
     1141 tagatacgcg tccatttttt gattttttca atgttttaaa aataagtgac acggaacttc
     1201 tgaagaataa ataggcgcct acaaccgtta aaaataaaat gataaacggt gatgtcatag
     1261 ccatagttag tgctttgaac gtaccaaaca tttctgtcgc catataatag cctagtgcaa
     1321 tcatagcaat acctaatacg cctgaaataa cctctgcagt cgttacttta gcagttgtgg
     1381 catcagtttt aattgaatct ttcatcattg ataagatact acgtcttttt agaaataaag
     1441 cacttttaaa taaaatcagt acatacgcaa taattagcat gaaaatagtt aaaacaaggg
     1501 ccataggttc gaaatgtatc gataagttaa tcgataacga catcaattta gatactatgg
     1561 aaagcaataa ttgtgcacct gcaatgccac ataatacacc gacaacacct gtgattaaaa
     1621 atacgatcat ttgttcaagt gctaacattt tcaaaatgtt ttgtcgtgtt aaaccaatca
     1681 actgaaatag cgcaaattca cgtgtacggc gttttacgaa taaatgattg gcatacatta
     1741 aaaagatgac aataatgata aataaaaata ttgatccgac taaagcacct ttcttaatga
     1801 tggccatcga gtcgtcatta tttacacctt tagtaaactg taaggttgta aaactgaaat
     1861 ataagacgat gctaaaaaat aatgaaaata aatacattgc ataatgtttt aagttttgtc
     1921 gtaagttttt gaaaacgata tggttaaatg tcatttgaga caccacctaa tactgattga
     1981 agatgtacaa tgtcttcata aaaggcctgt ttagaacgtc cttcctgata aagttgtgta
     2041 tgaatttggc catcttttag catgatgaca cgttctgcaa aacttgctgc aaccggatca
     2101 tgtgtaacca tgacaatagt tgtatcaaac gatttattca tttcttccaa acgttgtaat
     2161 aggtcatttg cacttttcga gtcgagtgcg cctgttggct catctgcaaa tatgatttgt
     2221 ggtttgtgaa caaatgctct cgctgctgca gttctttgtt gttgaccacc agataattcg
     2281 ctagggtatt tatttcctag gtcataaata cctaatgctg tcgtgatcgc tttataattt
     2341 tcttccattg ttgccttcga cattttttga acagataaag gtaacataat gttttcttta
     2401 acggttaatg tcggcagaat actgtaatct tggaagatga aacctaatga ttctttgcgg
     2461 aatttggcaa gtgctttttg attaagttta ttaagctctt gtccgttagc aatcacgcta
     2521 ccgctagaaa tttggtcaat tgaacttagt acatttaata aggttgtctt acctgatcca
     2581 gaaggcccca taatcgcaac gaattcgcct ttttgtatgt caaagttaat atctttaagt
     2641 gcttgaaatg tgtgcttttt accgtatgtt tttgaaacat gttgcactga taatatcgtc
     2701 atagtctcac tccttttgta tttaatttca ttttaaataa tgtttggagt agtagccttt
     2761 atctaaactt acaattcaat gaatgaacct tacagagttg aaagctatcg ctacttagta
     2821 gatttttgag tgaggataca gattcatcgt acatattaga caaaagcaat ggtgctttct
     2881 aagtgatgat gtttgtgtaa attgagaaaa gggaatttaa ttattgtata ataaattttt
     2941 tgtaaaaatt aaaagagggt tttatttgaa aggaattgat tgttatggaa aaaggaaatc
     3001 aaggtattaa atggtctagt ttaataatgg gtgtattatt attaatgttg gcagtcgtta
     3061 tttttacatt tccaattgaa aatttttatg ctattacctg gttgattgga ctgtttgtat
     3121 taattaacgg tgtgattcaa atcgtttacc gtagaaaagc aaaagcttta gtaggtggta
     3181 accaaaattg gattctgttt atggggattg tagatattct atttggtcta ttagttattt
     3241 ttaatgttgg cgcaagttca gcattcttta tttatatgtt tgctttttgg tttattttta
     3301 gttctatctc tggattattt acgttttcgg gtagtggtag cttaaaacta atttcagtga
     3361 tttttaattt attaggtatt gttttcggtg tcattttatt atttaatcca ttaatgggta
     3421 tcgtctttat ttcgacgatg attgctattg catttgtatt cgtaggtgtc atttatgttg
     3481 tagatgcact tgcttaagta aaatgaagcg gttcaaaaga agggtgtgac atgaagtttg
     3541 tgtcatatcc tttttgttgt gtttatgaag cataaaaaag gggcgctacc tacaataagt
     3601 aagatacgcc catattttta tattttacta ttattgtttt tcaatacgat taatagttac
     3661 atttagtcca aaatattttt ctaaaaaatg tttatagtta tctttagtga catcaaattt
     3721 ttctgagcta ccattccttg ttaaagttaa atgattttca gacattgtag cacggccaaa
     3781 tgattgtggc attgtaatta ataaatgctg tacaaatatt gaatctggat gcgtttgatt
     3841 atattcgatg ttgctattaa aatcttctat ttgtttaggt ttgaattcag cttcatattt
     3901 tgtatgccaa tgatcatttt cgaatttttg aacatagaaa atatccttgt cttcgttgtt
     3961 aaaaatagcg cggaatgtac cactgatatc agtaattggt tgtgtatgct cagatgaagt
     4021 aataggaatg gcatgtagag gtaagtctcc aaagccaaca tcagttacat agaatacatc
     4081 atttatagaa acaacaagtg aagcatgtga accgttcaga ctacgaccgc caccgggtgt
     4141 gtgaatagta gctgacatta attcaggatt aaatcctttt tgttgtaaat aggctttgaa
     4201 aaatgtattt aattcataac aaaaaccacc acgtttatca tgaacaattt tattaaaaag
     4261 tgcatcgata tttatagata tcggcttact attttgaaca tcaatatttt caaaaggtac
     4321 agttaacata aaacgtgttg cataataatt taatgcttca atactcggtc gattataacg
     4381 agatgaatca atttgtaaat aattctctaa cttcgcaata ttcataagca tagcgcctcc
     4441 tgtattaaag attataatta aattttaaac agaaatactg aaattttaaa ttcgaaagca
     4501 ttgaattttg gataaataca ttttaaatag aaaaatacgc tctcaaaatg aagtcatctc
     4561 taaaagaaac gatttaaaga tgactactga gagcgtagca taatggaaga agtgtgcagg
     4621 gtgtctaaaa atgcaacaat acaaaggtag ttgcaagaca agttgcctta tctagaccat
     4681 ttgtgttcta tgcgaccaaa cttccaaatt aaacttgaaa taagccaagt aattaaaaat
     4741 aatgcaacta aaatatagcc taaataatca aattcgatcg aaccaatgaa tgcccaaaac
     4801 gcaccatgta aatctaactt atcagcaaga atttgtagca attcaatcat cccaatcact
     4861 aatgctgcca tgactgatat cgcagtaatc gttatattgt aatagatttt gcgaatagga
     4921 ttgaagaatg cccaattata ggcatacttc attacaacac catctaatgt atccaataaa
     4981 ctcatacctg atgcgaataa aattggtaaa gataagattc cgataaatga aatggcttgt
     5041 tgtgatgcgc ctgaagaaag agcgagtaac gcaatttcac tagctgtatc aaaaccaagt
     5101 ccaaataaaa agccaagtgg caatacgtgc caactacgcg tgattaattt gaaataaggt
     5161 cctacaaatc gagaaaccaa tcctctagat tcaagtaatg catcgacttc agcttcttca
     5221 atgtgttcac gacgtaattt agcgaacaag ttaattaaag agattaaaat aattagattc
     5281 aacacaccga taagcactaa aaagaaacct gaaactagtg taccaatcgt tccaccaata
     5341 tcttggaaat gcggtaattc atctttagcc cattttacag atacccctaa aaaaacagcc
     5401 attaaaaata cgacagatga atgtccaatt gaaaaataga aacccacacc agatggatct
     5461 ttgcgttgct gtaataattt gcgaaccgta ttatctattg cagcaatgtg atctgcatca
     5521 aatgcatgac gcaaacctaa tgtatatgca agaatcccca taccaaataa gatatgatgg
     5581 tcttttccag caatccataa aaaactaaac ccaataacgt gtaacaaaat gacaatagct
     5641 atgtatggta accagcttaa gcgctcattt ttaaaaacag tcaaaatata tccttctttc
     5701 ataacatgat atttaatcat actgtatgtt caatgggcac tctagtaata agtgttcata
     5761 taacaaaaat gttatgccaa attatttgtt atataaaaat atacatgtaa ccacaaaaga
     5821 ttttttgcga tatatataat ttgataaatt aaccaacaac aatgtaagat gtcactttgc
     5881 ttaacttggc atccttttta tgattttcaa attcaaaaaa atgagcaaaa tgaatctctt
     5941 taccagtttt taatatttca ataccatgca tggaacctaa gcacccatgt gtgatgctgg
     6001 aatggatatt gagactagca acctgattgt aatgattaga tagttcttga attaatattt
     6061 gaggtccgta tatgtcaaag cggccaggga cagaccaaat aaattctgtt gtaaccagtg
     6121 aacgtaataa ttcaatatct aatgctgctg taacaactat aaaatctatc atttgttgac
     6181 gtttaggcgc atgattgcat gacacatctc ctgttaactt aaaaggtaat gatgactgaa
     6241 cttccgtttt aaaatgtagt tggtgctgaa ataaagcttg ttccaatatt gatatagctt
     6301 taggaccaac gccatgcatc tccattagag atgacttcgt atattgtgat actgcttcta
     6361 atgtgtatat accttgtgaa tttagcgcgc gtgttgcagg ctttccaatt ttaggtaaag
     6421 tcatttgtat ccatccttta tgttgtatgg tagtctttct ggcaatatac cctgatttgt
     6481 acagaattat ttataaaatt aatattgttt agtggttggt gaataaatgt gggaaatata
     6541 aacaaaatga tatctttatc gaaatattat tagtaaaatc gttatattag cataaccaag
     6601 tgagcgtatg cagtaaatta aaaattagga aagtaaggag gtttgaaggt gaattcagag
     6661 tataaaaaag gcatattttt agcactcagt gcatacattc tgtggggaat actacctata
     6721 tattggcagt tcgttgatgc aataggcgca tttgaaattt tagcctttcg tattatattt
     6781 tcagcaatat tcatgatttt catactcgcg gttggacaaa aacaacgcaa tgcatttcaa
     6841 cgagatatga atcaattgtt aggcaagccc attcagctat tagcgattgt cgtagcaggc
     6901 tatgtcatta cattaaattg gggtacattt atttgggctg taacgaacgg tcacgtccta
     6961 caaacaagtt taggttatta tataaatcca cttgttagca ttttgctcgc acttatcttt
     7021 ttaaaagaaa gattcaataa atttgaatgg ctagccattt tattcgcatt catcggtgta
     7081 ttatatatga cgctcaagat tggagaattc ccaatcgtct ctattatatt agcgttatcc
     7141 tttggtacat acggattatt gaaaaaagta gtacatattg atgccatcag cagtattacg
     7201 attgaatgta ttgttaccgc acctgctgga ctaatatacg ttatttattt atggcagcaa
     7261 catcagatgt catttggatt gaacatgtca tcattttggt tgttattttc tggtgctatt
     7321 acggcaatac cactaatcct attctcagcc ggggcaaaac gtattccact ttcgctaata
     7381 ggatttattc aatacgttgg accaacaata atgtttgtac tcggcatatt tgttttcaaa
     7441 gagcctttta gtatagatca attaattacg tttatattta tttggacagg tattgtgtta
     7501 tatagtcttt ctcaatacat taaattgaag aaacatccgg tcgcaaaaac cctataaaat
     7561 acgaggaaag ataattatac gtgttttcaa tatcctttgt gtcatgatga ggtgagactg
     7621 aggtatgtgt gcagtaacag tgacagacgt ataaatttga aacattaaat atgaaataag
     7681 gcactttcgt tgaattcgca attaaaatga atttaaacga aggtgttttt tgtatgatga
     7741 tttatgaggg catacgtaat gttaatcaat gaagcggaat agtatagaaa tgtattttat
     7801 gtgatgttac gtaatgactt aaatctttat acaaattttc agtgaagcac gcaatgattt
     7861 aaaggataaa cgtattttaa aaagggagtc ttgatttagc aaaggttgat tttaaataca
     7921 aattaaaaag cgcccaaatc aatgagattc gagcgctaat ttaaatattt taaaatatac
     7981 caattacttt catccaaagt gatcctaaac caatccagat aataaagtag acgaaaccta
     8041 atattaagtt cattgtccac caacgttttt gagtcacgta acctgaagag aatagaatcg
     8101 gcgctggacc actactatag tgtgttgttg aagctaatag gttaccgaag aaacctaaca
     8161 ttaatgcact gaataatggt ggtgcaccgg ctgcgatggc aacgcctagt aatgctgcat
     8221 acatcgcact gatatgtgct gtagaacttg caaataagta atgtgaatag aagtagaaca
     8281 atattaaaat gaccaggact ataggccagc ttaagccacc aagacttgta gcaatggatt
     8341 tacttaacca aggaataaat ccaagcttgt ttaattggtc ggccattaac actaatactg
     8401 agaaccatac taatgtgttc caagcacctg tttcgtttaa aatgtcttgc catgttaaga
     8461 cgcctgtcaa taataacaat gctagcgcaa taaaggccgt taaagttgca tcaatatgaa
     8521 tgaaacttcc gacaatccat agtgttaacg caacgacaaa aataccaatc ataaattttt
     8581 cagctaaagc gattttaccc atagtcgcta attcattttc agcccaactc ttagcatttg
     8641 gtgtttcttt aacagttggt ggataaattt tataaataat aaaaggtaca acaattaagg
     8701 aaacaagtcc aggaactaaa gcagctagaa accaattcat ccatgtaatg tgaacattag
     8761 atgtgctaga tgctaaattt tgtgcaaggg ggttaccggc cattgcagtt aaaaacatag
     8821 ccgcagtaat taaattacct tggaattctg tgaaaacaag aaatgcaccc attttgcgtg
     8881 ctgatccgtc tttcggtttc gaaccaaatg attcagaaag tgatttgata attgggaaca
     8941 tgattccacc agcacgcgcg gtattacttg gtgtagcagg cgctagaatt aaatctacac
     9001 cgacgataga atatgctaat cctaatgttt ttttaccaaa taatttgacg aaatgaagtg
     9061 cgatacgtct accaagacct gttttcacaa atcctctcga aatgaaaaat gccatagcaa
     9121 ttaaccaaat gctattatta ccaaaaccag cgacagccgt tttcatgtca acaatgccaa
     9181 cgagtaccat gattgtaaat ccaattatag agacggcccc aattggcatc ggttgtgtaa
     9241 tacaagcaat gattgtcgcg acgaatattg cgaacatata ccatgctgtt ggatccacag
     9301 catccggttt aaaaggtgta agtgcccaaa taaggagacc tacaacgata gggagtataa
     9361 acttacgata tttaaccgtg ttttccatgt taaaacgtcc ttctttctat gttttataca
     9421 tatttcaatt taagaataaa gctaactaca aaagatgtac agtaataatt aaatataaaa
     9481 ttaaattaac gaaatcatta atataattat ttttcgagaa tcggtgaaga actggtatag
     9541 atggtgttta ttaaattcaa aaaagtgacc ttttaaacaa ataaaaaacc cgttaacgtg
     9601 ttgttaacgg ttaataagca catatttatt ttaaattttt taatatacga gacatctctt
     9661 caattttacc agcatgaagt gggatgtgaa tggcatttaa tactaataac tcttctaagt
     9721 tattacaacc gacaattggc tgtttcaatt caattgctaa atcatcttca gttagatttc
     9781 gtgcacgttc aggtaaagtt tggagacctt ctaaaatcct ttcaatactc ggtacttcat
     9841 ccttccattc atctggagac gaaccatttc cgaataaagg tctatagata tttaaatcaa
     9901 tattctcttt accagcaaca gctaaggccg attcaaaaat tgttaataca tgaccaaatt
     9961 gccaatgaat ggtattagaa aaaggcttat gataatcatc aagtaccttt tcaactttcc
    10021 aagcatcata cacatttact aaatatccta accccatatc aatgacatca aatactgttt
    10081 ttgtagtcat tattaattcc tcccccataa ataaaatata cttccgtaag tattacccta
    10141 ttaacaaagt tgttagtcat ttaaggaatg tgtacaacaa ctaccttaca ataaaagata
    10201 gttgacgaaa agttctcaat gtactatctt attatgtaag atagtaggtg attaaaatgt
    10261 cagtgtcaga tcagattatg aaaggtctct tagatggggc gatattaggt ctcattgccc
    10321 aaggcgaaac gtatggatat gaaattatga cgaaacttaa gaatcaagag tttccagaga
    10381 taagtgaggg tagtatttat ccagttttaa tgcgtttaaa caaaaaagga tacgttgata
    10441 cgacaataaa aaaatctagt aatggcggcc cgaggcggaa atactatacg attaccgatt
    10501 caggtttaaa ggaactaaaa gtatttaaat cgaaatggca agttttgaat aaagggatga
    10561 ttaacttgtt tggaggggaa aacgatgtta agtaaacaat ctcaagattt tttagaaaaa
    10621 ttacgtgttg aattgttatt tagaggtaag actgaagcag aagtagaaga gttattggaa
    10681 gagctcgaag atcatttaac tatggctgaa aaaaataacg aagatacaag ttcaattatt
    10741 aatacaccga ttaagtcaat ggctgatcaa ttatcacctg agataagtct tactaccggt
    10801 ttgtataaat atattacttt gtacttcgct tttattttag cagtcattat aattcctagg
    10861 tttttagatt tgggtacatt tgatgtgaca attgcatttt tactttatat agctagtatc
    10921 gttattttag gtgttgtcat tggtatgctg attctacgaa atacattagt ccgatttggt
    10981 gaccaaaaaa taacgtatgt tataaatgtg acttatggaa ttatcgtatt tttatggatg
    11041 attttaggtg gcttgattat taaaaaatat cctatctata gtttctttga gttaagtgac
    11101 aaacagagtt tcatcgttgg gttagtactt ttaattgcgc tcattggaac ttgtatttta
    11161 attaaacaga aaatttatgc gctcatcgtg ttggtaattt cattgccaag tttgatagca
    11221 aggatagcag ctatctttag taaagaacca gacactttta atttagtttc agtaagtata
    11281 ctcatcattc taaatatagc atttattatt tatacatttg taaattttag gaagttaaaa
    11341 aaagaagact aaacaatgtc gtttagctaa aatacatcat tatagtaaaa ctgtatacca
    11401 atcatccaaa agcaccctaa agttagactt aaactaacaa attagggtgc ttttgtttgt
    11461 gcacttataa ctattccgtt gtgcccaggg ctaggtgtaa atcctcatca ttagaaatag
    11521 cttcaatggc tgcagtcaaa cctgcaacta tcttttctaa tggcatagat ggtgtatcgg
    11581 gtttaccaat gacctgctct ggtatgtatg gcacatgaat aaatccgaat cgtaggtgag
    11641 ggtaatgctt atcttgtaaa taacctaagt gataaagtgt gtgattacaa acaaatgtac
    11701 ctgcgctatt tgaaagtgct ccaggaagtc cttgattaat aatactttga gtcattgctt
    11761 taactggtaa atttgaaaaa taagctggcg caccgtctaa gtgaatggct tgatcaatag
    11821 gttgaaaatc atcattatct ggaatacgtg catcatcaat attaatggcg acacgttctg
    11881 gggtaatggc atttctacca ccagcttgtc ctatagctag tacaacatca taatgattag
    11941 atgccaacgt tttatttata atattatcta ctttcttaaa agaggttggt agttttaatt
    12001 tatcgattgt atgtgtgcca ataatatctt ctagttgagt cacagcttcc catgagggat
    12061 tgatattttg attgtcaaaa ggcgcaaacc ctgttactaa aatgtgcatt ttaagacacc
    12121 ccgcagtatt aaaatttaat ttagtataac acgcgcataa ttaaaaacat atcaggttat
    12181 gtatgcgcat aaaaaacagc cttgaaaaac ctttataaat cggattttca aagcgtgttt
    12241 tgaaaggtat ttaattaaaa ctaagcatgt tgatgtaaat catcaaagtt tgttaaacgt
    12301 tgttgccact cattatcact aatattattt gcttgaacat aacgattacg ctcatgttta
    12361 gcacattcat aagagcatgc acctaaatat ttagtttcgt tttcttcaga aactaatatt
    12421 tgtttattac attctgggtt agcgcaatta atataacgtt cacatggttt gccatcaaac
    12481 caatccttac caataattgt tttttcaact tggttgatat caacactgat acggtcatca
    12541 aatacgtaca ttttaccgtc ccaatattca ccttttgttt caggatcttt accatatgta
    12601 gcaataccgc catgaagttg agctacatct tcgaaacctt cttttaaaag ccatccagaa
    12661 aatttttcgc atcgaatgcc accagtacag tacgtaacca cttttttatc tgcaaataac
    12721 gctttattct ctttaatcca gtcaggcaaa tctctaaaac gtgtgatatt tggacgaatt
    12781 gcacctcgga aatgacctaa atcaaattca taatcattac gtgcatcaat aatgactgtg
    12841 tcatcatctt caagagcttt tctaaattct acaggtgata aatattggcc agttgtgtgt
    12901 cttggatcga cgtcatcttc taaatctaaa gcaacgattt cttttcgagg acgtacatgc
    12961 attttcttaa aagcatgtcc ttcagcttca tcaattttaa acaccatatc tttgaatcgt
    13021 tcatcggcat gcatatgtgc catatattgt tcggtttctt cttttgtacc agataatgtg
    13081 ccgttaatac cttctgtaga aacaagaatt ctacctttta aatggtgtgc tttacaaaag
    13141 gctaagtgat cctgagcaaa ctgttcaggg tcatcaatcg tcatatattt ataatataaa
    13201 agaacttgat agttcatagg ttaatctcct tctataaaca tgttataagc atgttaattt
    13261 ttaaaataaa gcgtcaatac gcatatttgt ctacgtccat cattttaaca gagttgtatt
    13321 gtcattcaat atttctagct tgaaatcagc cactagaccg ataaaataga tagatgaaac
    13381 cgttatcatt ttatttgata gttatcttgt ttaaaataaa attgatagtt aaaacgaatg
    13441 aaatgaaagg aatgatcatg atgtcacaac aagatttacc tacattattt tatagcggga
    13501 agtccaatag tgctgttcca attatatctg aaagtgaatt acaaacaatt acagctgaac
    13561 catggcttga aatttccaaa aaaggattgc aactagaagg attgaacttt gatcggcagg
    13621 gacaactctt tttattggat gtattcgaag gcaatatttt caaaatcaat cctgaaacga
    13681 aggaaatcaa acgacctttt gtaagtcaca aagcgaatcc tgcagcaatc aaaatacata
    13741 aagatggccg attattcgtt tgttatttag gagattttaa atctacagga ggcatttttg
    13801 cagctacaga aaatggtgac aacttacaag atattattga agatctttca acagcatatt
    13861 gtattgatga catggtattt gattctaaag gtggatttta ttttacagat tttagaggat
    13921 actctaccaa tccactagga ggcgtttatt atgtttcgcc ggactttaga acagtgacgc
    13981 ctatcattca aaatattagc gtagcaaatg gtattgcttt aagtacagat gaaaaagtac
    14041 tatgggtaac agaaacaaca gccaatcgat tacatcgcat tgcacttgaa gatgatggtg
    14101 tgacgataca accatttgga gctaccatac cgtactattt tacaggtcat gaaggaccag
    14161 actcatgttg tattgatagt gacgataatt tatacgtagc aatgtatggt caaggtcgag
    14221 tgttagtttt taataaaagg ggttatccaa taggacaaat attgatacca ggccgagatg
    14281 aagggcatat gttacgttct actcatccgc aatttatacc tggaacaaat caactcatca
    14341 tttgttccaa tgatatagaa atgggcggag gatctatgct ttatacagtt aatggctttg
    14401 caaaaggtca tcaaagtttt cagtttcaat aagacctacc tgaatgaaag gcgtctaaaa
    14461 ttagggtgaa cagttagtgc ttgttacaca tgagttaaac gaaaaatgcc aactatacct
    14521 agggatgata tagttggcat ttcgtatata tattaaaaca atgaggattg tgtaatgaga
    14581 ttattctgag attgagaatt cagctgatgc ttcgaattta acatctttgc ctagcattac
    14641 gccaccagtt tcaagtgctt ggttaaagtt aatgccatat ttttctctat tgattgtacc
    14701 agtaacaata atacctgtta cttgagaacc atccatagga ttcttactta ctccgttgaa
    14761 ttcaacatcg aatgtttctt cgttagtgat gcctttaatt gttaaatcac caacaacttt
    14821 gctttcagat actgattttg tcacaaatgt aattttatca aattcgtcag taccaaagaa
    14881 atcaccagat tttaagtggt tatcacgtgc ttcgttttta gtgttaattg agcttggaat
    14941 aattgttgca gtagctttca aagtactgaa gtcattaata tctccttcaa cagctacatc
    15001 aaattgatca aatgaacctt tcactttaga aaccattaaa tgtttaattt ggaattctaa
    15061 actactgtgt gcaccatcaa aagtaaagtt agtcataata taaaacctcc atatttttat
    15121 ctcttagata tattttttat acttgatata catacaatac cacctaaaaa acaaagatgc
    15181 aagcatttta tttcgaattc gaatattttc tgtgatgtat ttaaagaatg acctataaaa
    15241 caccacacta ttgttatacc ctagtcaatt aattcaaaca taaagaagtg attacaagtc
    15301 cgtgaaattt agttaatgag ataaatctga ctattattat catttttata gtcactacta
    15361 ttgactttgc atcaagtgac tcgtactatt ttaatataat tctgaaaatt caaaatatag
    15421 aacattaaat atgtagataa tatttttttg aagttttaaa aaaaggaggc attttatggg
    15481 aaattatttg gtattgcaat cagattttgg acttagtgat ggcgctgtaa gtgcaatgta
    15541 tggagttgca tatactgtaa gtgatgatat acgtgttgaa aacttaacgc atgatatacc
    15601 accgtatgac atttgggtag cgtcatatcg tttatatcaa actgtaaaat actggcctaa
    15661 aggcacagtt tttgtatcag tggtagaccc gggtgtaggt agtgataggc ggagcattgc
    15721 ttgcttaaca tattcaggtc attacattat tacacctgat aatggttctt taagtcatat
    15781 taaacattac gaaggtatta aaaaagttat cgaaattgat gaagtgaaaa gccgattgcc
    15841 tcattcggaa gaaagtcata cttttcatgg tagagatgtt tatgcataca atggtgcgag
    15901 attagctgca aataagatag cgtttgaacg tctcggtcag gcaattaatg ttgatagtat
    15961 tgaggcatta gaaataagag aagtgacaaa aaatgaggat tctgttacag gaagcattga
    16021 tattttagat atcagatttg ggtcattatg gacaaatatt ccattggcat tcttaaaatc
    16081 cttggaaatt gttcatggta ataatttggt ggtaacaatt tatcatcaag ataagaaagt
    16141 ctatcaaaat attattaaat ttgcgcgatc gtttgcagat gttaatgtgg gtgaacctct
    16201 tgtgtatgtt aactcgctag taaatattgg tgtagcagtc aatcaagact cattttcaga
    16261 tttatatcat attggtactg gaaatgattg gacgattcat ttatctaaag caccatcaat
    16321 ataatgaatt ttttatgtaa ggagtaatga agatgaaaaa acaagatatt tcagttaaaa
    16381 ctgttgttgc aattggtata ggagcagcgg tatttgtaat tttagggcgt tttgttgtaa
    16441 taccaacagg ttttcctaat acgaatatag aaacatcata tgcattttta gcattaatat
    16501 ctgcaatatt tggacctttt gctggtttaa tgactggatt agttggacat gctattaaag
    16561 attttacgac atacggtagt gcctggtgga gttgggttat ttgctcggga attataggct
    16621 gtttatatgg ctggattgga ctaaaattaa atctttcttc aggccgattt tctaggaaat
    16681 cgatggttta ttttaatatc ggtcaaatta ttgcgaatat tatttgctgg gcacttattg
    16741 caccaacatt agatattttg atttataacg aaccagctaa caaggtttat acacaaggtg
    16801 ttatctctgc agtattaaat attatttcag ttggcattat tgggacaata ttattaaaag
    16861 catatgcttc atctcaaatt aaaaaaggta gtttacgtaa ggaatagtta ttttgttgaa
    16921 tcagatatgt aaatgaatat agaaaggtaa tgatatatca tgactgaacc aattatctcg
    16981 ttcaaagact ttagttttca atatcatagt caagcaacac ctacattaca gaatataaat
    17041 gttgatattt atccaggaga aaaagtatta gtagttggtg cttcgggtag tggtaaatcg
    17101 acttttgcaa attgcataaa cggattaatt ccatttaaaa ctaaaggtaa cataactggg
    17161 gaactatata taaataatca agatgcaacc gttagttgtt tacatgatag atctaatgtt
    17221 gttggtacag ttttaaaaga tacagatgga cagttcatag gcttaacagc agctgaagat
    17281 atggcctttt tattagaaaa taattgtgtt gaacaagatg atatgaagaa aaatgtaagt
    17341 tattgggctg aaaaagttgg catgatagaa catttaaatc accgaccgca agatttatct
    17401 ggaggtcaaa aacaacgcgt ttcattaggt ggtatattaa tccatcgtac gcctatttta
    17461 atattggatg agccactggc caatttagat cctgcgacag gacatgaaac gctgagattg
    17521 ttaaacaata ttcatgaaga aacaaagtca acgatgatta ttgtcgaaca tcgattagaa
    17581 gaatcattag atgatacgtt tgatagagtt cttcttttta aagatggaaa aatcatcgct
    17641 aatacaacgc ctagtgattt gctaaaatca tctaagctta aagaagcagg catacgtgaa
    17701 ccattatact gtacagcgct taaatatgca gaagtagatg tcgaatccat tgataactta
    17761 gcgaatttac gtgacgtttg tatgagtgaa tatgttaaat ttaaagtgaa aaaatggata
    17821 gatgaaacat ctgcaaataa tgataataag tacaaatctg aaccgctatt agaattaaat
    17881 gaggtatgtg ttcaatatag tgactatagt aatagtgtac ttaataatgt tcaattaaat
    17941 gtttatagaa gagaaatgct tagtatagtt ggtcacaatg gtgccggcaa atcaacactt
    18001 gctaaagcaa tttgtggatt tttagatata acaggtaata tccaattttg taatagggga
    18061 tttaatcaat tgtcaattag cgaacgatct gaattcgtag gttatgtgat gcaaaatcca
    18121 aatcatatga tttctgaaaa aatgatttac gatgaagtag cattagggtt aagagcacga
    18181 ggtatgaaag aatcagatat aaagatacga gtagagaatg tgctcaaaat atgcggactt
    18241 tatgcatttc ggaattggcc aattgcagct ttaagctatg gtcaaaaaaa gagggtcact
    18301 atagcatctg ttttagtctt aaatccggaa ataatcatat tggatgaacc gactgctggt
    18361 caagatttct atcattataa tgagataatg tcatttttaa ttgaactaaa cagacagggg
    18421 aagacgatta ttatgattac gcatgatatg catttattgt ctgagtatag ttcaagaaca
    18481 gttgtattat caaaaggtca agtcgttgct gataccacgc cagtattggt tttaaatgat
    18541 aaaaaaatct gtgagattgc atcattgaga caaacatcgc tatttgaaat ggccgaatat
    18601 atagggatta gcgagccaca gaaattagta caattattta ttaaccatga taggaaggtg
    18661 agacgccaat gaatcaatat aatactatag gttttcaccc gggaaatagt cgtattcatc
    18721 aattaaatgc gactgttaaa cttttattct tattagttgt ttctatttct gcaatggtga
    18781 cttatgacac aagatattta attttaatta gtgcttcatc tattttattg gtcaaatatg
    18841 ctcatattga atggaaacaa gttcgctttg ttgttaaatt cattctgttt ttcacaatat
    18901 taaatattat tgccgtgtac atatttgacc ctgaatatgg tgtgaagatt tataatcagc
    18961 gtacagagtt agtcaatggt attggtcgat ttacgctaac atcacaggaa ttattctatc
    19021 tttttaatct aatattaaaa tatattagta cagttccttt agcgttaata tttttattca
    19081 caacgaatcc gagtcatttt gctgcaagtt taaatcagct aggtgtgaat tataaaatca
    19141 gttacgcagt ctcactagca ttaaggtata ttccagatat tcaagaaaca tattttaata
    19201 tttcacaagc gcaacaagca agaggatatg ataattcgaa aaaagcaaaa tttactagcc
    19261 gtgttaaagg tatcaaacgc attgtgttac ctttaatatt ttctagtatc gaaagaattg
    19321 acactattag tactgctatg gagttaagac aattcggaca gtataaaagg agaacctggt
    19381 acgtcaaaaa acaattaaaa aaagatgatt atgttgtttt gtgtttgacg ttaatacttc
    19441 tgatgttagt agttacatta ttctttttaa ataatagtcg atatttcaac ccgtggcatt
    19501 agtattcata taaatagtct ttaaatagaa ataggaggga gacatttaat gataaatact
    19561 gaaagattaa atttaatgat tccaagttcc tcgcatttaa ttgaacttta taatatttgt
    19621 agtcatccac aagcaaatat atacactccc aaaggtttac ataattccaa attagacaca
    19681 caacggtgga ttgaaaaatg gcgaaaccat tggcaacaat atcaatttgg ttactttgta
    19741 ttggtaaaaa aaatagattg tagtgttatt ggtatttgtg gatatgaata tcgacaatta
    19801 aagcaagaaa cagtattaaa tttattttat aaattacatc caagttttga aggacaaggg
    19861 tacgcatgtg aggctattac agcaatcaca aattttgtga attatatcga tcaagaaaca
    19921 gtaaaagtta tcaggacaaa taagtgtaac caacgttcaa taaatttagc agaaaggctt
    19981 aaattcaagc gagacgatac tatggacgac attatcaatc aaggagatat tgtgttttat
    20041 aaataaaata ctatgacatt atctaaaaaa taaaattaaa atcccaacag tttcttaaaa
    20101 agttatgagc cacataataa aaatgttata attgtgcata ataaaacata gggagaatgt
    20161 ttaatgcata aaaaatattt attgctattt tttctgttat tatcagttgc attgttatat
    20221 agctgttcca agcaaaatga gaaagctcaa aataaatctg ttaatccaaa tgagtatgca
    20281 aagcataaat cgaaaatgga tgaacattgg gaaacatatc acggccaagt ttatcatgtg
    20341 ttttatcacc cattaattac agacccaaaa gttgcgttta caggagatac acaccaagct
    20401 aagggcaaca atgattggat gattactgta agtgaattta aaaaatcatt gaacgaatta
    20461 tataaaaata attacatcat cgttaatcca catgatgtat acgacttatc atctcatcat
    20521 gtaaaagcca aaaaaataaa attgccgaaa ggtaaaaagc cactaatcat ctcaattgat
    20581 gatatgaatt actatagtta catgagacac catggttatg ctgaccgttt agtactcgat
    20641 aaaaataaac atgttgtttc tgaaactaca gacaaaaaag gccatgctac ttattctgat
    20701 gacaatgata tcgtgccaat tttaaataaa tttgtaaaag agcatccaga tttttcttta
    20761 aatggtgaaa aaggtgttgt tgctttaacc ggttatgagg gcgtattagg ttatcgcacg
    20821 aatgaattaa cgagtaaaga ttatacaaag aataaaaagg cagctgagga agttgttcgc
    20881 gcaatgaaac gggatggatg gtcatttgcg agtcattcat atggtcacat taattttgaa
    20941 aaaacatctt tagaaggtat taaacgtgat actaaaagat ggaaagatga agttgaacca
    21001 attgtaggta aaacagatat gtttgtattt ccacatggcg cacaggatag acatacacaa
    21061 gcgtatgatt atttggtaga tgaagcagag tttaagttta ttgcaggtgt cggtccaaac
    21121 aattttacag atatatcagc aacgaacgtt taccaagata gggttgcgat tgatggtcta
    21181 aacttatttg aatttaaata taaactcaaa ccgtttttta atcctgaaaa tgtatatagt
    21241 aaacaagata gacgttactt taaagggaat cgggattatg aagaatagtt aaatttaata
    21301 aaaagataga aagaaaataa ctttacataa taaggcaagg acaatatgtg caagaagtac
    21361 aacttcaata taaaaatctt ggttaagcat ttttataggc acaaatgtct tgcctttttt
    21421 attttaaaaa attttaaaat caatacaccg aattttctga aaaaggttgt aatgtattgc
    21481 taaaaatgat aagttattaa tcatattaca ctttagcgtg gtagagaagt aaagcaaaca
    21541 acgaaaatac caagtgattt taaatacagt attgaaagta aagggtggtc gagtgaataa
    21601 ttcagaacaa ttaattgcgt taaaagaatc ggaaacagca tttttaaaat attttaataa
    21661 agcggattat gagttagttg attttagtgt ggttgaaaag ttggactgga aacaattgaa
    21721 ccatgaggat ttgcagcaga tgggtgagcg aaatttttgg caacatgagc atcaaattta
    21781 tgcacttcga aatgatttta cagatcaatt attgcgttat tactcaatgt atccaactgc
    21841 agcaacaaaa gtggcataca caggtttaat cattcggaat aatgaagcgg ctgtacaagt
    21901 tggtttggaa aactatgcac catcattagc gaatgtgcaa cagagcttaa agctctttat
    21961 tcagtttatt caacagcagt tgagagacaa cgtgcatttt gttgtattgg gacattatca
    22021 attgcttgat gccttactcg ataaatcgtt acaaacacca gacatactat caatgattga
    22081 ggaacgtaat ttatcgggac tggttacata tttgtcgact gaacatccaa tcgtgcaaat
    22141 cttgaaagag aatacgcaac aacaattaaa tgtattggaa cactatattc caaatgacca
    22201 tccagcactc gttgaattaa aaatatggga acgttggtta cataaacaag gttacaaaga
    22261 catccattta gatattactg cgcagccacc tagatcctat tacacaggtt tatttattca
    22321 atgtcatttt gctgaaaatg aatctcgagt tttaacaggt ggttattaca aaggaagcat
    22381 cgaagggttt ggattaggat taacacttta agtaagggag tatgcacaat gttaagaatc
    22441 gccatagcca aaggacgtct aatggatagt ttaattaact atttagatgt aattgaatat
    22501 acgacattat cagaaacatt aaaaaataga gaacgccaat tattattaag tgtagataat
    22561 attgaatgca ttttagtaaa aggaagtgac gtgccaatct atgtggaaca aggaatggca
    22621 gacataggca ttgttggtag cgacatatta gatgagcgcc aatataatgt taataatttg
    22681 ttgaatatgc cttttggagc atgtcatttt gcggttgcag cgaaacctga aacgaccaat
    22741 tatcgtaaaa tcgcaacgag ttatgttcat actgctgaaa catattttaa atcaaaaggt
    22801 attgatgtcg aattgattaa attgaatggc tctgttgaat tggccggtgt tgtagatatg
    22861 gtagacggaa ttgtcgacat cgttcaaaca ggtactacgc taaaagcgaa cggactggtt
    22921 gaaaagcaac atattagtga tatcaatgca agattaataa ctaataaagc agcttatttt
    22981 aaaaaatcac aattaataga gcaatttatt cgctctttgg aggtgtctat tgccaatgct
    23041 taatgcacaa caatttttaa atcaattttc attagaagca ccattagatg agtcattgta
    23101 tccaattatt cgcgatattt gtcaggaagt taaagttcat ggagataagg ctttgaaaat
    23161 gtataatcta acattcgatc atacgaaaac agatcattta gaaattagtc atgaacaaat
    23221 taaagcagca tttgacacat tagatgaaaa aacaaaacaa gcattacaac aaagttatga
    23281 aagaattaaa gcatatcaag aaagtattaa acagacgaat caacagttag aagaatcagt
    23341 ggagtgttat gaaatatacc atccactaga aagtgtcggt atttatgtgc ctggtggtaa
    23401 agcaagttat ccttcaacgg tattaatgac agcgacgtta gcacaagtag caggtgtgga
    23461 aaatattgtc gttgtgacac cacctcaacc taatggtgtg tctcaagaag tattagcagc
    23521 atgctatatt acgcaagttg atcaagtatt tcaagttggt ggtgctcaaa gtattgctgc
    23581 gttaacttat ggcacagaaa cgatacctaa agttgataag attgtaggtc caggcaatca
    23641 atttgttgca tatgccaaga aatatttatt tggacaagta ggtattgacc aaatagctgg
    23701 accaacagaa atagcactta ttattgatga aacagcagat ttagatgcca tcgtatatga
    23761 tgtttttgca caagcagaac atgatgaatt agcacgtaca tatgccatta gtgaagatgc
    23821 gcaagtcctt aaagatttag aatcacgtat tgctaaagca ttgcctaatg tggacagata
    23881 cgacattgtt tctaaaagta tcgctaatca acactacctt atccatgcta gtaattttga
    23941 tgaagcatgc catgtcatga atacaatcgc gcctgaacat gcgtcgattc aaacagtaaa
    24001 tcctcaacca tatattgaaa aagtgaaata tgtgggtgca ttgtttattg gacattattc
    24061 gccagaggtc ataggagatt acgttgcagg tccaagtcat gtattaccta caaatagaac
    24121 agctagattt accaatgggt tatcggtcaa tgatttctta acacggaaca cggtcatcca
    24181 tttatcaaaa gatacgtttg aacaaattgc tgattcagca caacatattg ctcatgttga
    24241 agcattatac aatcaccagc agtctatttt aatacgtcag tcttagggga gtgtaattga
    24301 aatgatttat attgataaaa atgaaagtcc agttacgccg ttggatgaaa aaacaatgac
    24361 gtctattatt agtgcaacgc catataattt atatcctgat gcagcatatg aacaattcaa
    24421 ggaagcttat gctaagtttt acggattatc gcctgaacaa attattgcag gaaatggatc
    24481 tgatgaattg attcaaaagt taatgctgat catgccagaa ggtccggcat taacgctaaa
    24541 tcctgatttt tttatgtatc aagcatatgc ggcacaagta aatcgtgaaa ttgcatttgt
    24601 agatgcagga tcagatttaa cgtttgattt ggaaaccatt ttaacgaaaa tcgatgaagt
    24661 acaaccatca ttttttatta tgagtaatcc acataaccct tcaggcaagc aatttgatac
    24721 ggcattttta acagctattg cagataagat gaaagcatta aacggatact ttgtcattga
    24781 tgaagcatat ttagattatg gtacggcata tgacgtggaa ctggcaccac acatcttaag
    24841 aatgcgtaca ttatcaaagg cgtttggaat tgccggctta agattaggtg tcttaattag
    24901 tactgctgga acgataaagc atattcaaaa aatagaacat ccatatccat taaatgtatt
    24961 tacgctaaat attgcgactt atatttttag acatagagaa gagacaagac aatttttaac
    25021 gatgcaacga cagttagctg agcagttaaa acaaatattt gatacacatg ttgcagataa
    25081 aatgtcagtg ttcccatcaa atgctaattt tgtacttact aaaggctcag cagcgcaaca
    25141 attaggacaa tacgtatatg aacaaggatt taaacctcgc ttttatgatg agccggtgat
    25201 gaagggctat gtaagatact caattgcaac agcatcacag ttaaagcaat tagaagaaat
    25261 tgttaaagaa tggagtgcaa aatatgattt atcaaaaaca acgaaacaca gctgaaacgc
    25321 aactaaatat ttcaatatct gatgatcagt caccatcgca tattaataca ggtgtgggct
    25381 ttttaaatca tatgttaacc ttgtttacat ttcatagcgg tctgtcatta aacattgagg
    25441 cacaaggtga tattgacgta gatgatcacc acgtaactga agatatcggc attgtcattg
    25501 gccaattgtt acttgaaatg attaaagata aaaagcattt cgttcgttat ggaacgatgt
    25561 acattccaat ggatgaaaca ttagcacgtg tcgttgtgga tataagtggg cgcccatacc
    25621 tatcattcaa tgcatcatta agtaaagaaa aagttggtac gtttgatacg gagttagtag
    25681 aagaattttt tagagcggtc gtaatcaatg caagattaac aacgcatatt gatttaattc
    25741 gtggaggtaa tacacaccat gaaattgaag ctatattcaa agcgttttcc cgtgcattag
    25801 gcatagcgct aactgcaact gatgatcagc gtgtgccgtc atcgaaaggt gtgattgaat
    25861 gattgtcatc gttgattatg gattagggaa tattagtaat gtaaaacgcg ctattgaaca
    25921 tttagggtat gaggtggttg tctcaaatac ctcaaaaata atcgatcaag cagaaacaat
    25981 catattgccc ggtgtcggcc attttaaaga tgcgatgtca gagataaaac gattaaatct
    26041 caatgcaata ttggctaaga atactgataa gaagatgatt ggtatttgtt taggcatgca
    26101 attaatgtat gagcatagtg atgaaggcga tgcatctgga ttagggttta tcccaggaaa
    26161 tatttcgcgt atccaaacag aatacccagt gccacactta ggctggaata atttagtgag
    26221 taagcaccct atgttaaatc aagatgttta tttcgtacat tcttatcaag cgccgatgtc
    26281 agaaaatgta attgcatatg cgcagtatgg ggctgatatt ccggcaattg ttcaatttaa
    26341 caattatatt ggtattcaat tccatcctga aaaaagcggt acatatgggt tacaaatttt
    26401 gcgtcaggca atacaagggg gatttataaa tgattgaatt atggccagcg attgatttga
    26461 ttgggtcaac aagtgtgagg ttaacagagg gtaaatatga tagtgaagaa aaaatgtcac
    26521 gctcggctga agaaagtatt gcttactata gtcaatttga atgtgtgaat cgtattcata
    26581 tcgtcgactt gataggtgct aaggcacagc atgcccgaga gtttgattat attaagtcat
    26641 tgaggagatt aacaaccaaa gatattgaag taggtggtgg cattcgtacg aagtcacaaa
    26701 tcatggacta ctttgccgca gggattaatt attgcatagt tggaacgaaa ggtattcaag
    26761 atactgattg gcttaaagag atggcacata catttccagg tcgcatttat ttatctgttg
    26821 atgcctatgg agaagatatt aaagtgaacg gatgggaaga ggacacagag ttaaatttat
    26881 ttagttttgt gagacggtta tcggatatac ctcttggcgg cattatatat actgatattg
    26941 ctaaagatgg caaaatgtcc ggacctaact ttgaattaac tggtcaatta gtaaaggcaa
    27001 cgacgattcc tgtcattgct tccggtggta ttagacatca gcaagatatt caacgattag
    27061 cgtcgctaaa tgttcacgct gctattatag gaaaggctgc acatcaagca tctttttggg
    27121 aggggctaaa atgattaaaa aacgtatcat tccatgttta gatgtcaaag atggtcgtgt
    27181 cgttaaaggg attcaattta aaggattaag ggatattggg aatcctgttg atttagcaat
    27241 gtattacaat gaagcgggtg ctgatgaatt agtattttta gacatctcta agacggaaga
    27301 gggtcatagc ttaatgctag aagtgattga acagacagcg tcacgcttgt ttatccctct
    27361 tactgtaggg ggtgggattc aaagtctcga tgatattacc caattgctaa atcatggtgc
    27421 agataaagta tcattaaatt caagtgcttt aaaaaatcca cagctcatta aacaagcgag
    27481 tgataaattc ggtagacaat gcatctgcat agcaattgat agctattatg atcctgaaag
    27541 aaaagcacat tattgttgta cgactggtgg taaaaaaatg acaaatatta aagtatatga
    27601 ctgggtacag caagtagaac agttaggtgc aggtgagctc ctcgttacaa gtatgggaca
    27661 tgatggtatg aaacaaggct ttgatattga acacctagca aatattaagt ctcttgtaaa
    27721 tattccaatc attgcttctg gtggtggtgg caatgcacaa cactttgtag aattatttga
    27781 tcagacggat gtttctgcag gtttagctgc aagtatatta catgatcgag aaacgacggt
    27841 tcaatctatt aaagaagtga tacggcaagg gggtatagca gtaagatgac caaatataaa
    27901 attgatttta gcaaaggttt agtgccagca attttacaag ataatcaaac aaaacaagta
    27961 ttgatgttgg gttatatgaa ccaagaagct tttgataaaa cgatagaaga tggtgtggta
    28021 tgtttctatt cgcggtcgaa acaacggcta tggacaaaag gtgaaacatc tggtcatacg
    28081 caacgtgtta aagatattca tgtagattgc gacaatgaca ctattttaat tgatgtcata
    28141 ccaaatggac caacatgtca tacaggcagt caaagttgtt tcaacacaga agttccattt
    28201 tcagtgcaaa cattagcgca gacagttcaa gatagtgccc aatccaataa tgaaaagtca
    28261 tatacaaaat atttattaac agaaggtata gaaaagatta caaaaaaata cggtgaagaa
    28321 gcttttgaag tcgtaattga agcaattaaa ggtgacaaaa aagcatttgt aagtgaagta
    28381 gcagatgaac tttatcattt atttgtcttg atgcatgcgc ttggcgtcga tttttcagaa
    28441 attgaggcgg aattagcgcg tagacatcat aagcgcaata actttaaagg tgaacgacaa
    28501 aatatcgaac agtggtaaag caagtatgga ctaagatata aggaaaagga tcatggctta
    28561 tacacttaca aatattgtgg aaaacgtgac attttcaagt ttaaaatacg acaccaacat
    28621 attttaacta tgaatgctgt gatggtacta aagttgcgaa ctcgttatag ataagtagtg
    28681 gataatcaca atacgaaatc aaaaataatt ataaaaagta aattgagcaa ctcaggaata
    28741 gatgtcactg ttaaagatgt cgaaaagtat atgaatcgat ataatgaagt tatgaaggga
    28801 aaaaatggcg aaaaagctaa agagttatgt ttgtcgttac tacctattaa tatcatagtt
    28861 gtctttacat tctttgtatt tatactataa atacaaatat atctagcctg aaatagaaat
    28921 gtcatagcct atttaaaaga caatctccat tagaactaag atatgcatcc cgaaagttag
    28981 actaaaaaac taactttatg ggatgtattt ttatgctaat catcataaat tcgagattaa
    29041 gttaaaggta gttcaagagt aattaaacaa taaattaaaa atagtaggat acttactttg
    29101 agggaagaaa attaactgta tatatttagt ttaggaacaa gtattacggt ttatcctgat
    29161 acaattattg tggatgggat gatattttta ggtttaaaat acgacaccag caaacataat
    29221 aactgtaata gctcataaat ctccccatat agctaatcta aaaaaataat acatcattgg
    29281 aattaagccc caagcatgta aatattaaaa atcaaaaaag atatatgtaa aaaagttaca
    29341 atttgcataa ttaaattgtg tctaattatt gactaattaa attttgccaa atataatatt
    29401 aattaataat ttgaaatgat tagcgtatac actttaaatt ctctttggag aatatatttt
    29461 ttaaatacaa atgtaaacgc tttctcgtca aattaaacaa tagaaaggat ggtcattatg
    29521 agtgcttggt taagtaaatt atttgagttt attcctcgaa taattatcaa tttgtttatc
    29581 taaaataaaa aaatagaggt gctgacaatg atgaaaagtc aaaataagta tagtattcgt
    29641 aaatttagtg taggtgcatc ttccatttta atagctacat tactattttt aagtggtgga
    29701 caagcacaag cagctgagaa gcaagtgaat atgggaaatt cacaggagga tacagttaca
    29761 gcacaatcta ttggggatca acaaactagg gaaaatgcta attatcaacg tgaaaacggt
    29821 gttgacgaac agcaacatac tgaaaattta actaagaact tgcataatga taaaacaata
    29881 tcagaagaaa atcatcgtaa aacagatgat ttgaataaag atcaactaaa ggatgataaa
    29941 aaatcatcgc ttaataataa aaatattcaa cgtgatacaa caaaaaataa caatgctaat
    30001 cctagcgatg taaatcaagg gttagaacag gctattaatg atggtaaaca aagtaaagtg
    30061 gcgtcacagc aacagtcaaa agaggcagat aatagtcaag attcaaacgc taataacaat
    30121 ctaccttcac aaagtcgaat aaaggaagca ccatcattaa ataagttaga tcaaacaagt
    30181 caacgagaaa ttgttaatga gacagaaata gagaaagtac aaccacaaca aaataatcaa
    30241 gcgaatgata aaattactaa ctacaatttt aacaatgaac aagaagtgaa acctcaaaaa
    30301 gacgaaaaaa cactatcagt ttcagattta aaaaacaatc aaaaatcacc agtagaacca
    30361 acaaaggaca atgacaagaa aaatggatta aatttattaa aaagtagtgc agtagcaacg
    30421 ttaccaaaca aagggacaaa ggaacttact gcaaaagcga aagatgatca aacgaataaa
    30481 gttgccaaac aagggcagta taaaaatcag gatcctatcg ttttagtgca tggtttcaat
    30541 gggtttacag atgatattaa tccttcagtg ttagctcatt attggggcgg taataaaatg
    30601 aacattcgcc aagatttaga agaaaatggt tacaaagctt atgaagcaag tataagtgct
    30661 tttggaagta actatgaccg cgcagttgaa ctttattatt atatcaaagg cggtcgtgta
    30721 gattatggtg cagcacatgc agcaaaatat ggacatgaac gttatggaaa aacatacgaa
    30781 ggaatttaca aagactggaa accaggacag aaggtacacc tagttggaca tagtatgggc
    30841 ggtcaaacga tacgtcaact agaagaatta ctgcgtaatg gtaatcgtga agaaatagag
    30901 tatcaaaaga aacatggtgg cgaaatttct ccactattca aaggtaatca tgacaatatg
    30961 atttcatcaa ttactacttt aggaacacca cataatggta cacacgcatc agatttagct
    31021 ggtaatgaag ctttagtgag acaaatcgta tttgatatcg gtaaaatgtt tggtaataaa
    31081 aattcaagag tagacttcgg gttggctcaa tggggtctaa aacagaagcc aaatgaatca
    31141 tatattgatt atgtcaaacg cgttaaacaa tctaatttat ggaaatcaaa agataatgga
    31201 ttttacgatc tgacgcgtga gggtgcaaca gatttaaatc gtaaaacgtc gttgaaccct
    31261 aacattgtgt ataaaacata cactggtgaa gcaacgcaca aagcattaaa tagcgataga
    31321 caaaaagcag acttaaatat gtttttccca tttgtgatta ctggtaactt aatcggtaaa
    31381 gctactgaaa aagaatggcg agaaaacgat ggtttagtat ccgttatttc ttctcaacat
    31441 ccatttaatc aagcttatac aaaagcgaca gataaaattc aaaaaggcat ttggcaagtg
    31501 acgcctacaa aacatgattg ggatcatgtt gactttgtag gacaagacag ttctgataca
    31561 gtgcgcacaa gagaagaatt acaagatttt tggcatcatt tagcagacga tttagtgaaa
    31621 actgaaaagc tgactgatac taagcaagca taatttataa agtaaaggga ggaattaata
    31681 atgactgcag acttctttca attaatcgga tcattattta gaattctaaa agaattattc
    31741 aagtaaaaca ttggcgaggc cccaacataa agaatttcga aaagaaattc tacaaacaat
    31801 gcaagttggc ggggccccaa caaagaagct ggcggaaagt cagcttacaa taatgtgcaa
    31861 gttggcgggg ccccaacata gaagctggcg gaaagtcagg ttacaataat gtgcaagttg
    31921 gggtgggacg acgaaataaa ttttgcgaaa atatcatttc tgtcccactc ccattggcat
    31981 ttacgaagtt taaatgtgca attagaatat atgtataaca atattaaaca cgcggtaaaa
    32041 cgaagtcagt caattcaaac tgatttcgcc caccgcgtgt ttttaacata gcttaataat
    32101 taataagcat taatgttcaa tttatatggt tgttttccaa taataaacct aaagatatag
    32161 aattcacgca atatcatgcc gacacctata cataatccta aaatgaatag tagtgatatc
    32221 gctagaaaga ccattgtatt atcctcaaat atatttgtat atgcaaacaa tgagtctaga
    32281 atgattggat gtaataaata aataaagaat gagaaagcac taatcatttg aatcgtatta
    32341 aataacattg ttttaaaatg cgtgcaaata cccaagataa caataaacat aatactatta
    32401 tatggtgtta atgaatatga aaagctggta acgttccaat agtctccatt tgctaacgca
    32461 ataaacacaa aataagtagc tacagctaat acaatcataa taactaaata acgttctaag
    32521 aaatttaata cacgttcgta gttataaccc atatatgcac ctaagaagaa ataaaaaatc
    32581 catccgaata ttatagtatt ttcact
//
LOCUS       DAKTIO010000012        30527 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.012, whole genome shotgun sequence.
ACCESSION   DAKTIO010000012 DAKTIO010000000
VERSION     DAKTIO010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 30527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30527
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.012"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            117..1148
                     /locus_tag="O0293_000416"
     CDS             117..1148
                     /locus_tag="O0293_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCY0279326.1"
                     /translation="MDLLIGTLFLFLVLVIFTLFTYKAPNGMRAMGALANAAIATFLV
                     EAFNKYVGGEVFGIKFLEELGDAAGGLGGVAAAGLTALAIGVSPVYALVIAAACGGMD
                     LLPGFFAGYMIGYVMKYTEKYVPDGVDLIGSIVILAPLARLIAVLLTPVVNSTLIRIG
                     DIIQSSTNTNPIIMGIILGGIITVVGTAPLSSMALTALLGLTGVPMAIGAMAAFSSAF
                     MNGTLFHRLKLGDRKSTIAVSIEPLSQADIVSANPIPIYITNFFGGAIAGLIIAMSGL
                     INDATGTATPIAGFLVMFGFNHPTTIVIYGVVMAIVGALAGYLGSIVFKKYPIVTKQD
                     MINRGAVDA"
     gene            1491..2609
                     /locus_tag="O0293_000417"
     CDS             1491..2609
                     /locus_tag="O0293_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCY0279327.1"
                     /translation="MSDSEKEILKRIKDNPFISQRELAEAIGLSRPSVANIISGLIQK
                     EYVMGKAYVLNEDYPIVCIGAANVDRKFYVHKNLVAETSNPVTSTRSIGGVARNIAEN
                     LGRLGETVAFLSASGQDSEWEMIKRLSTPFMNLDHVQQFENASTGSYTALISKEGDMT
                     YGLADMEVFDYITPEFLIKRSHLLKKAKCIIVDLNLGKEALNFLCAYTTKHQIKLVIT
                     TVSSPKMKNMPDSLHAIDWIITNKDETETYLNLKIESTDDLKIAAKRWNDLGVKNVIV
                     TNGVKELIYRSGEEEIIKSVMPSNSVKDVTGAGDSFCAAVVYSWLNGMSTEDILIAGM
                     VNAKKTIETKYTVRQNLDQQQLYHDMEDYKNGKFTKVY"
     gene            2584..3507
                     /locus_tag="O0293_000418"
     CDS             2584..3507
                     /locus_tag="O0293_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCY0279328.1"
                     /translation="MANLQKYIEYSREVQQARENNQPIVALESTIISHGMPYPQNVEM
                     ATTVEQIIRNNGAIPATIAIIDGKIKIGLESEDLEILATSKDVAKVSRRDLAEVIAMK
                     CVGATTVATTMICAAMAGIQFFVTGGIGGVHKGAEHTMDISADLEELSKTNVTVICAG
                     AKSILDLPKTMEYLETKGVPVIGYQTNELPAFFTRESGVKLTSSVETPERLADIHLTK
                     QQLNLEGGIVVANPIPYEHALSKAYIEAIINEAVVEAENQGIKGKDATPFLLGKIVEK
                     TNGKSLAANIKLVENNAALGAKIAVAVNKLL"
     gene            3518..4738
                     /locus_tag="O0293_000419"
     CDS             3518..4738
                     /locus_tag="O0293_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCY0279329.1"
                     /translation="MNILFAITGIAFALFVAFLFSFDRKKIDFKKTLIMIFIQVLIVL
                     FMMNTTIGLTILTALGSFFEGLINISKAGINFVFGDIQNKNGFTFFLNVLLPLVFISV
                     LIGIFNYIKVLPFIIKYVGIAINKITRMGRLESYFAISTAMFGQPEVYLTIKDIIPRL
                     SRAKLYTIATSGMSAVSMAMLGSYMQMIEPKFVVTAVMLNIFSALIIASVINPYKSDD
                     TDVEIDNLTKSTETKTLNGKTGKPKKVAFFQMIGDSAMDGFKIAVVVAVMLLAFISLM
                     EAINIMFGSVGLNFKQLIGYVFAPIAFLMGIPWSEAVPAGSLMATKLITNEFVAMLDF
                     KNVLGDVSARTQGIISVYLVSFANFGTVGIIVGSIKGISDKQGEKVASFAMRLLLGST
                     LASIISGSIIGLVL"
     gene            complement(4843..6375)
                     /locus_tag="O0293_000420"
     CDS             complement(4843..6375)
                     /locus_tag="O0293_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HCY0279330.1"
                     /translation="MKEVGFGTLNWVAVIIYLLAMLFIGVYFTKRASQSTNSFFTASG
                     RLPSWVVGFSIYATTLSAITFMSTPEKAFLTDWSYIAGNIAIVAIIPLLIYFYVPFFK
                     KLKVTSAYEYLEARFGPSIRVIGSLLFVVYHLGRVAIVIYLPTLAITSVSDMNPYIVA
                     SLVGLLCILYTFLGGFEGVVWSDFIQGVILLGGALVIIILGVVNIKGGFGTVFADAIE
                     HKKLISADNWKLNTAAAAIPIIFLGNIFNNLYQYTASQDVVQRYQASDSLKETNKSLW
                     TNGILALISAPLFYGMGTMLYSFYTHEAVLPKGFNTSSVVPYFILTEMPPFVAGLLIA
                     AIFAAAQSTISSSLNSISACISIDIKQRFFGKGSERHEVNFARFIIIIAGIFGFGMSL
                     YLIASNSNDLWDLFLFVTGLFGVPLAGVFAVGIFTKRTNTFGVICGLILGIIFAYVYN
                     GVGKGNSPFYVSTISFTVAFVFAYILSFIVPSKHKKDITGLTIFEKDKPSTYISKTAT
                     KK"
     gene            complement(6415..7296)
                     /locus_tag="O0293_000421"
     CDS             complement(6415..7296)
                     /locus_tag="O0293_000421"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCY0279331.1"
                     /translation="MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLY
                     VNGSSGENFLLNTEQKKQVFKVAKEAVGDKVKLIAQVGSLDLNEAIELGKYATELGYD
                     ALSAVTPFYYPFTFEEIRDYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEK
                     IVGVKYTAPNFFLLERIRKAFPDKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRAR
                     KIFDLARQGQIQEAYQLQHDSNDIIETVLSMGIYPTLKEILRHRGIDAGLPKRPFKPF
                     NEAHRQTLDQLIAKYDL"
     gene            7454..8314
                     /locus_tag="O0293_000422"
     CDS             7454..8314
                     /locus_tag="O0293_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCY0279332.1"
                     /translation="MYYIAIDIGGTQIKSAVIDKQLNMFDYQQISTPDNKSELITDKV
                     YEIVTGYMKQYQLIQPVIGISSAGVVDEQKGEIVYAGPTIPNYKGTNFKRLLKSLSPY
                     VKVKNDVNAALLGELKLHQYQAERIFCMTLGTGIGGAYKNNQGHIDNGELHKANEVGY
                     LLYRPTENTTFEQRAATSALKKRMIAGGFTRSTHVPVLFEAAEEGDDIAKQILNEWAE
                     DVAEGIAQIQVMYDPGLILIGGGISEQGDNLIKYIEPKVAHYLPKDYVYAPIQTTKSK
                     NDAALYGCLQ"
     gene            complement(8592..9392)
                     /locus_tag="O0293_000423"
     CDS             complement(8592..9392)
                     /locus_tag="O0293_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCY0279333.1"
                     /translation="MKFENRVQRYQHLFTKTDKRIVNYIRQNGYSDAFSTINSLAHAI
                     GTSPATMTRFSHKLDYENFQDLKFNIQQEMTETVIENSPIIQRIHKYHQQIIQQTGEF
                     IDNDIIQTFIDKLQSSRHILFAGLGSSGLSATEFYYRMIRMGLKGNVTTDSHLMKISA
                     SLLSHSDMFIAMSNSGNTSELISAAEVAKSHGAYVVAITNFEGSKLTDCADLVLLTTD
                     QSCNTDHQFINTQIATLFLIDIVSYHLLENTNLSQTYQHTKSIILDNK"
     gene            9532..10200
                     /locus_tag="O0293_000424"
     CDS             9532..10200
                     /locus_tag="O0293_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCY0279334.1"
                     /translation="MLPHGLIVSCQALPDEPLHSSFIMSKMALAAYEGGAVGIRANTK
                     EDILAIKETVDLPVIGIVKRDYDHSDVFITATSKEVDELIESQCEVIALDATLQQRPK
                     ETLDELVSYIRTHAPNVEIMADIATVEEAKNAARLGFDYIGTTLHGYTSYTQGQLLYQ
                     NDFQFLKDVLQSVDAKVIAEGNVITPDMYKRVMDLGVHCSVVGGAITRPKEITKRFVQ
                     IMED"
     gene            complement(10327..11640)
                     /locus_tag="O0293_000425"
     CDS             complement(10327..11640)
                     /locus_tag="O0293_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCY0279335.1"
                     /translation="MNQYHSNAQQPSAWRFFVYSLVGILCFFIPFTINGNNTIFVDHV
                     HLAIRSIIGPLMPYVALIMILIGTALPIVRRTFMTSITNLVITLFKVAGAMIGIMYVF
                     KIGPSILFKANYGPFLFEKLMMPLSILIPVGAIALSLLVGYGLLEFVGVYMEPIMRPI
                     FKTPGKSAVDAVASFVGSYSLGLLITNRVYKQGMYNKREATIIATGFSTVSATFMIIV
                     AKTLGLMPHWNLYFWITLVITFVVTAITAWLPPISNESTEYYNGQEGEQEVAIEGSRL
                     KTAYAEAMKQNALTPSLVKNVWDNLKDGLEMTVGILPSILSIGFLGLIVANYTPFIDW
                     LGYIFYPFIYIFPIADQALLAKASAISIVEMFLPSLLVTKAAMSTKFVVGVVSVSAII
                     FFSALVPCILATEIKIPVWKLIIIWFLRVALSLLITIPVALLIFG"
     gene            12058..14130
                     /gene="lip2"
                     /locus_tag="O0293_000426"
     CDS             12058..14130
                     /gene="lip2"
                     /locus_tag="O0293_000426"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip2/Geh"
                     /protein_id="HCY0279336.1"
                     /translation="MLRGQEERKYSIRKYSIGVVSVLAATMFVVSSHEAQASEKTSTN
                     AAAQKETLNQPGEQGNAITSHQMQSGKQLDDMHKENGKSGTVTEGKDTLQSSKHQSTQ
                     NSKTIRMQNDNQVKQDSERQGSKQSHQNNATNNTERQNDQVQNTHHAERNGSQSTTSQ
                     SNDVDKSQPSIPAQKVIPNHDKAAPTSTTPPSNDKTAPKSTKAQDATTDKHPNQQDTH
                     QPAHQIIDAKQDDTVRQSEQKPQVGDLSKHIDGQNSPEKPTDKNTDNKQLIKDALQAP
                     KTRSTTNAAADAKKVRPLKANQVQPLNKYPVVFVHGFLGLVGDNAPALYPNYWGGNKF
                     KVIEELRKQGYNVHQASVSAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKT
                     YKGIMPNWEPGKKVHLVGHSMGGQTIRLMEEFLRNGNKEEIAYHKAHGGEISPLFTGG
                     HNNMVASITTLATPHNGSQAADKFGNTEAVRKIMFALNRFMGNKYSNIDLGLTQWGFK
                     QLPNESYIDYIKRVSKSKIWTSDDNAAYDLTLDGSAKLNNMTSMNPNITYTTYTGVSS
                     HTGPLGYENPDLGTFFLMATTSRIIGHDAREEWRKNDGVVPVISSLHPSNQPFVNVTN
                     DEPATRRGIWQVKPIIQGWDHVDFIGVDFLDFKRKGAELANFYTGIINDLLRVEATES
                     KGTQLKAS"
     gene            complement(14372..15199)
                     /locus_tag="O0293_000427"
     CDS             complement(14372..15199)
                     /locus_tag="O0293_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0279337.1"
                     /translation="MTEIKDKVITKDAFALPYTIIKAKNQPTKGAIVYIHGGGLMFGK
                     ANDLSPQYIDILTEHYDLIQLSYRLLPEVSLDCIIEDVYASFDAIQSQYSNCPIFTFG
                     RSSGAYLSLLIARDRDIDGVIDFYGYSRINTEPFKTTNSYYAKIAQSVNETMIAQLTS
                     PTPVVQDQIAQRFLIYVYARGTGKWINMINIADYTNSKYNIAPDELKTLPPVFIAHCN
                     GDYDVPVEESEHIMNHVPHSTFERVNKNEHDFDRRPNDEAITIYRKVVDFLNAITMM"
     gene            complement(15272..16471)
                     /locus_tag="O0293_000428"
     CDS             complement(15272..16471)
                     /locus_tag="O0293_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCY0279338.1"
                     /translation="MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNTSTKEGYITKEDL
                     AYAARRSNSAGMQVTGAAYIEPYGQLFEYGFNIDHDACIPGLTNMASTMKQHGSLAII
                     QLAHAGRFSNQAILNFGKVYGPSPMTLHSPIEHDVIAMSHEKINSIIQQYRDATLRAI
                     KAGFDGVEISIAQRLLIQTFFSTFSNKRTDHYGADSLKNRARLCLEVMRAVQEVIDKE
                     APDNFILGFRATPEETRGSDLGYTIDEFNQLINWVMDVSNIQYLAIASWGRHIYQNTS
                     RTPGDHFGRPVNQIVYEHLAGRIPLIASGGINSPESALDALQHADMVGMSSPFVTEPD
                     FVHKLAEQRPHDINLEFSMADLEDLAIPHAAFKDIVKMMDYGEGLKKHTRDALRQLEQ
                     NYHDSSS"
     gene            16567..16659
                     /locus_tag="O0293_000429"
     CDS             16567..16659
                     /locus_tag="O0293_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279339.1"
                     /translation="MESIFKIKLMNGICRSENMNMKKKNKGEKI"
     gene            16773..17774
                     /locus_tag="O0293_000430"
     CDS             16773..17774
                     /locus_tag="O0293_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCY0279340.1"
                     /translation="MVKLSVLDYALIDEGKDAQKALQDSVTLAKLADRLGFKRIWFTE
                     HHNVPAFACSSPELLMMHTLAQTNHIRVGSGGVMLPHYRPYKIAEHFRMMAALYPNRI
                     DLGIGNNPGTTMVKQALDGINPTYDSYDESISLLRDYLTIKDKPSAHTLGVQPHIDHF
                     PEMWLLSSSATSAKIAAELGIGLSVGTFLLPDINAIHAAKDNIDIYKKHFQASTIKMD
                     AKVMASVFVIVADNEAEVAALQHALDVWLLGKLQFAEFEDFPSVDTAQKYKLNDRDKE
                     MIQAHQARIIAGTQEKVKAQLDDFIATFEVDEVLVAPLIPGIEQRCKTLKLLAEIYL"
     gene            17808..18140
                     /locus_tag="O0293_000431"
     CDS             17808..18140
                     /locus_tag="O0293_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HCY0279341.1"
                     /translation="MKKLANYLWVEKVGDLYVFSMTPELQDDIGTVGYVEFVSPDEVK
                     VDDEIVSIEASKTVIDVQTPLSGTIIERNTKAEEEPTILNSEKPEENWLFKLDDVDKE
                     AFLALPEA"
     gene            18141..18941
                     /locus_tag="O0293_000432"
     CDS             18141..18941
                     /locus_tag="O0293_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HCY0279342.1"
                     /translation="METLKSNKARLEYLINDMHRERNDNDVLVMPSSFEDLWELYRGL
                     ANVRPALPVSDEYLAVQDAMLSDLNRQHVTDLKDLKPIKGDNIFVWQGDITTLKIDAI
                     VNAANSRFLGCMQANHDCIDNIIHTKAGVQVRLDCAEIIRQQGRNEGVGKAKITRGYN
                     LSAKYIIHTVGPQIRRLPVSKMNQDLLAKCYLSCLKLADQHSLNHVAFCCISTGVFAF
                     PQDEAAEIAVRTVESYLKETNSTLKVVFNVFTDKDLQLYKEAFNRDAE"
     gene            18931..19875
                     /locus_tag="O0293_000433"
     CDS             18931..19875
                     /locus_tag="O0293_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HCY0279343.1"
                     /translation="MQSSKWNAMSLLMDDKTKQAEVLRTAIDEADAIVIGIGAGMSAS
                     DGFTYVGERFTENFPDFIEKYRFFDMLQASLHPYGSWQEYWAFESRFITLNYLDQPVG
                     QSYLALKSLVEGKQYHIITTNADNAFDVADYDMTHVFHIQGEYILQQCSQHCHAQTYR
                     NDDLIRKMVVAQQDMLIPWEMIPRCPKCDAPMEVNKRKAEVGMVEDAEFHAQLHRYNA
                     FLEQHQDDKVLYLEIGIGYTTPQFVKHPFQRMTRKNENALYMTMNKKAYRIPNSIQER
                     TIHLTEDISTLITAALRNDSTTKNNNIGETEDVLNRTD"
     gene            19853..20875
                     /locus_tag="O0293_000434"
     CDS             19853..20875
                     /locus_tag="O0293_000434"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCY0279344.1"
                     /translation="MYLIEPIRNGEYITDGAIALAMQVYVNQHIFLDEDILFPYYCDP
                     KVEIGRFQNTAIEVNQDYIDKHSIQVVRRDTGGGAVYVDKGAVNMCCILEQDTSIYGD
                     FQRFYQPAIKALHTLGATDVVQSGRNDLTLNGKKVSGAAMTLMNNRIYGGYSLLLDVN
                     YEAMDKVLKPNRKKIASKGIKSVRARVGHLREALDEKYRDITIEEFKNLMVTQILGID
                     DIKEAKRYELTDADWEAIDELADKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISV
                     EQNRIADCRIYGDFFGQGDIKDVEEALQGTKMTREDLTHQLKQLDIVYYFSNVTVEAL
                     VDMILS"
     gene            21190..22215
                     /locus_tag="O0293_000435"
     CDS             21190..22215
                     /locus_tag="O0293_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HCY0279345.1"
                     /translation="MNNKVLATGGTGFVGMRIISRLLEQGYDVQTTIRDLSKADKVIK
                     TMQDNGISTERLMFVEADLSQDEHWDEAMKDCKYVLSVASPVFFGKTDDAEVMAKPAI
                     EGIQRILRAAEHAGVKRVVMTANFGAVGFSNKDKNSITNESHWTNEDEPGLSVYEKSK
                     LLAEKAAWDFVENENTTVEFATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQI
                     PLNVVDVRDVAELHILAMTNEQANGKRFIATADGQINLLEIAKLIKEKRPEIAQKVST
                     KKLPDFILSLGAKFNHQAKEGKLLLDMNRNVSNERAKTLLGWEPIATQEEAILAAVDS
                     MAKYHLI"
     gene            complement(22308..23654)
                     /locus_tag="O0293_000436"
     CDS             complement(22308..23654)
                     /locus_tag="O0293_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCY0279346.1"
                     /translation="MQAILNFIVDILSQPAILVALIAFIGLIVQKKPAATITSGTIKT
                     ILGFLILSAGADVVVRSLEPFGKIFQHAFGVQGIVPNNEAIVSLALKDFGTTAALIMV
                     CGMIVNILIARFTNLKYIFLTGHHTFYMAAFLAIILTVSHIKGWLTIVIGALVLGLIM
                     AVLPALLQPTMRKITGNDQVALGHFGSISYFAAGAVGQLFKGKSKSTEEIKFPKGLSF
                     LRESTISISITMALLYFIACLFAGVSYVHESISDGQNFIVFSLIQGVTFAAGVFIILT
                     GVRLILAEIVPAFKGISEKLVPNSKPALDCPIVFPYAQNAVLIGFFVSFITGVIGMFI
                     LFLFGGVVILPGVVAHFFLGATAAVFGNARGGIKGAIAGAALNGILITFLPLLFLPFL
                     GELGGAATTFSDTDFLAVGIVFGNAVKYMGLFGAILFIIIVGATTILLKGRQKEQQ"
     gene            complement(23669..23953)
                     /locus_tag="O0293_000437"
     CDS             complement(23669..23953)
                     /locus_tag="O0293_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCY0279347.1"
                     /translation="MKILVVCGHGLGSSFMVEMNAQEALRQLNAPSDIEVEHSDIMTA
                     SPEMADLFICGRDLAENAERLGDVLVLDNILDKAELQQKLSEKLQQLNMI"
     gene            complement(23955..24398)
                     /locus_tag="O0293_000438"
     CDS             complement(23955..24398)
                     /locus_tag="O0293_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCY0279348.1"
                     /translation="MSLDILSTTRIIVKEQVNDWTEAITIASQPLLQEQIIEQGYVQA
                     MIDSVNELGPYIVIAPEIAIAHARPNNDVHQVGLSLLKLNQHVAFCDEDHYASLIFVL
                     SAIDNHSHLSVLQNLATVLGDNQTVQQLLTATNAQDIKNILKEHD"
     gene            complement(24403..26358)
                     /locus_tag="O0293_000439"
     CDS             complement(24403..26358)
                     /locus_tag="O0293_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCY0279349.1"
                     /translation="MLSYRQIEILYLLIHEQTFIPINTIANQLGVSPRTIQYDIAYIE
                     QYAETYHYQVSRNKAAGIKVTTAHATLLNELEHNLTNQIHFSKDERLTHIALKLFETT
                     DPVSTKQLAQDVNVSRRTIADDIKMIQAQLDQYHLKLNYVHNKGFNIIGEEDHYRKAY
                     AHFIHQYMKQAAPFIEADIFNSESIALVRRAIIKTLNSENYHLVQSAIDGLIYHILIA
                     IQRLNENFSFDIPINEIDKWRHTNQYAIASKMIENLERSCNVTFPESEIIFITLHLLG
                     SKMTEHTASSITFEYHDLSQNIHELITCVSQELGIDMSKDNKLHTSLITHIKPAIHRI
                     KYDMLQPNPLRQEVMRRYPQIIEAVSKHISPIEQDAAIRFNEDELTYITIHFASSIER
                     VATHKQSMIKVVLLCGSGIGTSQLLKSKLNHLYPEFHIWDAYSIYQLEESRLLQDNID
                     YVISTVPCEISAVPVIHVDPFINQQSRQKLNQIINDSREQRVMKMATDGKSLADLLPE
                     HRIIINKQPLSIESAIAVAVQPLINDGIVYSNYTAAILKQFEQFGSYMVISPHIALIH
                     AGTDYVQNGVGFALTYFTEGIIFGSKANDPVHLVITLATDHPNAHLKALGQLSECLSN
                     DLYRQDFLDGNIFKIKQHIALTMTKEA"
     gene            26570..26989
                     /gene="mepR"
                     /locus_tag="O0293_000440"
     CDS             26570..26989
                     /gene="mepR"
                     /locus_tag="O0293_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor MepR"
                     /protein_id="HCY0279350.1"
                     /translation="MEFTYSYLFRMISHEMKQKADQKLEQFDITNEQGHTLGYLYAHQ
                     QDGLTQNDIAKALQRTGPTVSNLLRNLERKKLIYRYVDAQDTRRKNIGLTTSGIKLVE
                     AFTSIFDEMEQTLVSQLSEEENEQMKANLTKMLSSLQ"
     gene            27096..28451
                     /gene="mepA"
                     /locus_tag="O0293_000441"
     CDS             27096..28451
                     /gene="mepA"
                     /locus_tag="O0293_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498905.1"
                     /note="MepA (multidrug export protein A), as described
                     originally in Staphylococcus aureus, is a MATE transporter
                     shown to be over-expressed after culturing in the presence
                     of tigecycline, owing to mutation of its repressor MepR,
                     and then to be one of several factors contributing to
                     elevated tigecycline resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MepA"
                     /protein_id="HCY0279351.1"
                     /translation="MKDEQLYYFEKSPVFKAMMHFSLPMMIGTLLSVIYGILNIYFIG
                     FLEDSHMISAISLTLPVFAILMGLGNLFGVGAGTYISRLLGAKDYSKSKFVSSFSIYG
                     GIALGLIVILVTLPFSDQIAAILGARGETLALTSNYLKVMFLSAPFVILFFILEQFAR
                     AIGAPMVSMIGMLASVGLNIILDPILIFGFDLNVVGAALGTAISNVAAALFFIIYFMK
                     NSDVVSVNIKLAKPNKEMLSEIFKIGIPAFLMSILMGFTGLVLNLFLAHYGNFAIASY
                     GISFRLVQFPELIIMGLCEGVVPLIAYNFMANKGRMKDVIKAVIMSIGVIFVVCMSAV
                     FTIGHHMVGLFTTDQAIVEMATFILKVTMASLLLNGIGFLFTGMLQATGQGRGATIMA
                     ILQGAIIIPVLFIMNALFGLTGVIWSLLIAESLCALAAMLIVYLLRDRLTVDTSELIE
                     G"
     gene            28555..28995
                     /locus_tag="O0293_000442"
     CDS             28555..28995
                     /locus_tag="O0293_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HCY0279352.1"
                     /translation="MCKSKILLKNIFSEESEVKDLTEEKYNQDYEALTFSFKEETYQS
                     RLAKKTPTKSGYFVTCWTKDEDNYNRPYKIEEFADYLIVAVIDDELNGYFLFPRELLV
                     EKGILASSKYQGKMAFRVYPKWCNQLNKTAGQTQKWQCKYFFEY"
     gene            complement(29078..30436)
                     /gene="glpT"
                     /locus_tag="O0293_000443"
     CDS             complement(29078..30436)
                     /gene="glpT"
                     /locus_tag="O0293_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCY0279353.1"
                     /translation="MNFLKPAKHIKPLPENQIDDTYKRLRLQVFLGIFIGYAGYYLLR
                     KNFSLAMPALQEQGFTKAELGFALSAVSIAYGFSKFFMGTVSDRSNARIFLVLGLALT
                     AIVNLLMGFVPFFTSGIGIMFVLLFLNGWFQGMGWPPSGRVLVHWFSVSERGSKTALW
                     NVAHNVGGGIMAPIAAWGITTTAFINFGYLKGFEGVFIYPALLALIIAAISYVLIRDT
                     PQSQGLPPIEIYKNDFATSDKKTLETELTTKEILFKYVLNNKWVWAIAFANIFVYFVR
                     YGVLDWAPVYLSEEKHFDLKASGWAYFLYEWAGIPGTLLCGYISDKLFKGRRGPAGFF
                     FMLGVTVFVLIYWLNPPGNAWLDNVSLIAIGFLIYGPVMLIGLQALDYVPKKAAGTAA
                     GLTGLFGYLFGAVMANIVLGAVVDKFGWDVGFILLTAISVFAMLSFILTWNKVGQETV
                     HH"
BASE COUNT        10332 a         4799 c         5909 g         9487 t
ORIGIN      
        1 gaaaatcaga aatattccgt gaaatataat attttttgta gtaaaatggc ctctaagtat
       61 tcaatattta aatatgggga ttgaatataa aattatcgta atgggggtca atggttatgg
      121 atttattgat aggtacttta tttttatttt tggtcttagt gatttttaca ttatttacat
      181 ataaagcgcc taatggtatg cgtgccatgg gagcattagc taatgcagca atcgcaacat
      241 ttttagtgga agcatttaat aaatatgttg gtggcgaagt attcggtatt aaatttttag
      301 aagagctagg agacgctgcg ggaggtctag gtggtgtcgc tgccgctgga ttaacagcat
      361 tagctatcgg tgtgtcacca gtatatgcat tagttatagc agccgcgtgc ggtggtatgg
      421 atttattacc aggtttcttt gcgggttata tgattggata tgtgatgaaa tatacagaga
      481 aatatgtgcc ggatggtgtc gacttaattg gatcgattgt catcttagcg ccattagctc
      541 gtcttattgc agtattatta acgccagtag tgaatagtac attgattcga attggtgata
      601 ttatccaaag tagtacgaat acgaatccaa ttatcatggg tatcatttta ggtggtatta
      661 ttacggttgt cggcacagcg ccattgagtt caatggcatt gacagcatta ttaggtttaa
      721 cgggtgtacc tatggctatt ggtgccatgg cagcatttag ttcggcattt atgaatggga
      781 cgctattcca tcgcttaaaa ttaggtgatc gtaagtctac gattgcagta agtattgaac
      841 ctttatcaca agcagatatt gtatcagcca atccaattcc aatctatatt acaaatttct
      901 ttggtggtgc gattgctggt ttaattattg ctatgtcagg tttaattaac gatgcgacag
      961 gtacagctac accgattgca ggatttttag ttatgtttgg atttaatcat ccgacgacaa
     1021 ttgtgattta tggtgtagta atggcgattg taggtgcgct tgcaggttat cttggttcaa
     1081 ttgtatttaa aaaatatcca attgttacta agcaagacat gattaatcga ggtgcagtag
     1141 acgcatagca tcatcatatt gaatagtaaa aacaaataaa acatagtaac gtgattcagt
     1201 cgatgtaaca gtcgataatg agtcacgttt ttttatagaa aaatacaaga cataaaaatg
     1261 tcataattta ttgtcgacaa atatcatact gtataaacat ttatcatttt ctcaagtacc
     1321 ttttacacga tggaatgaac ttacttttta cgaaattatg cgtattttat aaacaaatat
     1381 cattgatata acggtaaatg taagcgttta caacagaaat aacagcatgc tacgatattt
     1441 ttgtaaattc actgattcaa gtattttaag tcaatatgag gagggatgtt atgagcgatt
     1501 ctgagaaaga aattttaaaa agaattaaag ataatccgtt tatttcacaa cgtgaacttg
     1561 ctgaggcaat tggattatct agacccagcg tagcaaacat tatttcagga ttaatacaaa
     1621 aggaatatgt tatgggaaag gcatatgttt taaatgaaga ttatcctatt gtttgtattg
     1681 gcgcagcgaa tgtagatcgt aagttttatg tgcataaaaa tttagttgca gaaacatcaa
     1741 atcctgtaac gtcaacacgc tctattggtg gcgtagcaag aaatattgct gagaacttag
     1801 gtaggcttgg cgaaacggtc gcttttttat ctgctagtgg acaagatagt gaatgggaaa
     1861 tgattaaacg attgtccaca ccatttatga atttggatca tgttcaacaa tttgaaaatg
     1921 cgagtacagg ttcatataca gctttaatta gtaaagaagg cgacatgaca tatggcttag
     1981 cagatatgga agtgtttgac tacattacgc ctgaattttt aattaagcgt tcacacttat
     2041 tgaaaaaggc taagtgcatt attgtagatt tgaatttagg caaagaggca ttaaacttct
     2101 tatgtgccta taccacgaaa catcaaatca aattagttat caccacggtt tcttccccaa
     2161 aaatgaaaaa tatgcctgat tcattacatg ctattgattg gattatcacg aataaagatg
     2221 aaacagaaac atacttaaat ttaaaaatag aatctactga tgatttaaaa atagctgcta
     2281 aacgctggaa tgatttaggt gttaaaaatg ttattgtgac aaatggcgtg aaagaactca
     2341 tttatcgaag tggtgaggaa gaaatcatta agtcagttat gccatcaaat agtgtgaaag
     2401 atgttacagg tgcaggcgat tcattctgtg ctgcagtagt gtatagctgg ttaaatggga
     2461 tgtctactga agatatatta attgctggta tggttaacgc aaagaaaacg atagaaacga
     2521 aatatacagt taggcaaaac ctagatcaac agcaacttta tcacgatatg gaggattata
     2581 aaaatggcaa atttacaaaa gtatattgag tattctcgag aagttcagca agcacgggag
     2641 aacaatcaac cgattgtagc attagaatca acaattattt cgcatggtat gccgtaccca
     2701 caaaatgttg aaatggcaac aacagtagag caaattatca ggaataatgg tgccattcca
     2761 gcaaccatag ccattataga tggcaaaatt aaaattggtt tagaaagcga agatttagaa
     2821 atactggcaa ctagtaaaga cgttgctaaa gtatctagaa gggatttagc agaagttatt
     2881 gcgatgaagt gtgttggtgc tactactgta gcgacgacga tgatatgtgc tgcaatggct
     2941 ggtattcaat tttttgttac aggaggtatt gggggcgtcc ataaaggtgc agaacatacg
     3001 atggacattt cagcagactt agaagaactg tctaaaacaa atgtcactgt tatctgtgca
     3061 ggtgccaaat caattttaga cttacctaag acgatggagt atttagaaac aaaaggcgtt
     3121 ccagttattg gatatcaaac gaatgaattg ccagcattct tcactcgcga aagcggtgtt
     3181 aagttaacaa gttcggttga aacgccagaa cgacttgctg acattcattt aacaaaacag
     3241 cagttaaatc ttgaaggtgg cattgttgtt gctaatccaa ttccatatga gcatgcctta
     3301 tcaaaagcat atattgaggc aatcataaat gaagctgttg ttgaagcgga aaatcaaggt
     3361 attaaaggta aggacgccac accgttcttg ttagggaaaa ttgtagaaaa aacgaatggt
     3421 aaaagtttag cagcaaatat aaaacttgtt gaaaacaatg cggcgttggg tgctaaaatt
     3481 gctgtcgctg ttaataaatt attgtaggtg atgatacatg aatattttat tcgctatcac
     3541 agggatagca tttgcactat ttgttgcgtt tttattcagt tttgatcgta aaaaaataga
     3601 cttcaaaaag acgttaataa tgatatttat tcaagtgttg atcgtgttat ttatgatgaa
     3661 cacaacgatt ggtttgacaa ttttaactgc actaggttca ttttttgaag ggctaataaa
     3721 tattagtaaa gcaggcataa attttgtttt tggagatata caaaataaaa atggctttac
     3781 gttcttttta aacgtattac tgccattagt ttttatttct gtattaatag gcatctttaa
     3841 ttatattaag gtattaccat ttattatcaa atatgtaggt atcgctatta ataaaataac
     3901 tagaatgggg cgcttagaaa gttattttgc tatttcaaca gcaatgtttg ggcaaccaga
     3961 agtatattta acaataaaag atattattcc aagattatct agagcgaaat tatatacaat
     4021 tgcgacgtct ggtatgagtg ctgttagtat ggcaatgcta ggttcatata tgcagatgat
     4081 tgaacccaag ttcgtagtta cagcagtaat gttaaatatt tttagtgcgc ttatcatcgc
     4141 cagtgtaatc aatccctata aatctgatga tactgatgtt gaaattgata acttaacgaa
     4201 atccacagaa actaaaacat tgaatggaaa aacaggaaaa cctaagaaag ttgccttttt
     4261 ccaaatgatt ggtgatagtg cgatggatgg gtttaaaatc gctgttgtag tagccgtaat
     4321 gttgttagca tttatttcat taatggaagc aattaatatc atgtttggta gtgttggttt
     4381 gaactttaaa cagcttattg gctatgtgtt tgcaccaatc gcattcttaa tggggattcc
     4441 atggagcgaa gctgttccag ctggctcttt aatggcgact aaattaatta caaatgagtt
     4501 tgtagcaatg cttgatttta aaaatgtcct gggtgatgta tcagctcgaa cacaaggtat
     4561 catttcagtt tacttagtaa gcttcgctaa ttttggtacg gttggtatca tcgtaggttc
     4621 aattaaaggc attagtgata aacaaggaga aaaagttgca tcctttgcaa tgaggttgct
     4681 acttggttca actctagctt caatcatttc aggatcaatc attggcttag tattgtaaat
     4741 gaatcgaagt acctaaatta aattcatggc aaagctaaac cccgtcacca agttggcgca
     4801 acagcgcatc ataacttagt gacggggttt tatcataaca atctactttt tcgtagccgt
     4861 ttttgaaatg tatgttgatg gtttatcttt ttcaaaaatt gttaatcccg ttatatcttt
     4921 tttatgtttt gaagggacaa tgaagctaag tatataagca aagacaaaag caactgtaaa
     4981 tgaaatggta gatacataga aaggtgagtt acctttgcca acaccattat agacataagc
     5041 aaagatgata cccaatatta atccacaaat aacaccgaat gtattcgtac gtttagtgaa
     5101 aataccaact gcaaatacac cagccaatgg aacgccgaat aatccagtca caaacaagaa
     5161 taaatcccat aagtcatttg aattagaagc aattaagtat agtgacattc caaaaccgaa
     5221 aatacctgca atgataataa tgaaacgtgc aaagttaact tcgtgtcgct cgctaccttt
     5281 tccgaagaag cgttgcttaa tgtcgattga aatacaagca gatatagaat ttaaactaga
     5341 tgaaatggta gactgtgcag cggcgaaaat ggctgcaata agtaatcctg ctacaaatgg
     5401 tggcatctca gtcaaaatga aatatggcac tacagatgat gtattgaagc cttttggtaa
     5461 aacagcttca tgtgtataaa atgaatacag cattgtaccc ataccataaa ataagggtgc
     5521 tgaaattaaa gctaggatac catttgtcca taacgattta tttgtttctt ttaaactatc
     5581 agaagcttga taacgctgca cgacgtcttg actcgctgtg tattgataca agttgttgaa
     5641 aatatttcct aggaaaataa ttggaatggc agctgccgca gtatttagtt tccaattgtc
     5701 tgcactaatt aattttttgt gctcaatcgc atctgcaaag acagtgccga aaccgccttt
     5761 aatgttcaca acacctagaa taataataac taaagcgccg cctaataaaa tgacgccttg
     5821 aatgaaatca ctccaaacca caccttcgaa accacctaaa aatgtatata aaatacatag
     5881 taaaccaacg agtgatgcaa cgatataagg gttcatgtct gatacagatg tgattgctaa
     5941 tgttggtaag tagataacaa ttgcaacacg ccctaaatgg taaacgacaa ataataatga
     6001 gccaatgaca cgtatgctag ggccaaatct agcttctaaa tattcatatg cagatgttac
     6061 ctttaacttt ttaaagaaag ggacatagaa ataaataagt aatggaataa ttgcgacgat
     6121 agcaatgtta ccagcgatat atgaccaatc tgttaaaaat gctttctctg gtgtcgacat
     6181 aaatgtaatc gcacttaacg tagtagcata aattgaaaag ccaactaccc aagatggcaa
     6241 gcgaccactt gcggtaaaga aactattggt actttggctc gcgcgcttgg taaaataaac
     6301 gccaatgaac aacatagcta gtagataaat gataacggca acccagttta gtgtgccaaa
     6361 tccaacttct ttcatgggca acatcccctt tacaatgtat tgattctttg atgtctataa
     6421 atcgtatttt gcaatgagtt gatctaatgt ttgtcgatgt gcttcgttaa aaggtttgaa
     6481 aggtcttttc ggtaatcctg catcaatgcc acgatgacgt aatatttctt tcaatgttgg
     6541 ataaatcccc attgataaca ctgtttcgat aatgtcgttt gaatcatgtt gcagttggta
     6601 agcttcttga atttgacctt gtcgtgctaa gtcgaagatt tttcttgcac ggcgaccatt
     6661 aacgttatat gtagaaccaa ttgcaccatc tacgccagaa atcgtagctt gaactaacat
     6721 ttcatcaaag ccagataaga ttaatttgtc tgggaatgct tttctaatac gttcgagtag
     6781 gaagaagttt ggcgctgtat atttaacacc aacaattttt tcatgattaa atagctcgct
     6841 gaattgttca atagaaatat tcacacctgt taaatctggt attgcataaa taatcatatt
     6901 gttctgagtt gcttcgataa tatcgaaata gtaatctcta atttcttcaa aagtaaatgg
     6961 atagtagaat ggtgttacgg cagaaagtgc atcataaccg agttctgtgg catattttcc
     7021 aagttcaatg gcttcattta aatctaacga acctacttga gcaatcaatt tcactttatc
     7081 cccaactgcc tctttggcaa ccttgaaaac ttgcttcttc tgctctgtat ttaataaaaa
     7141 gttttcgcct gagctaccat ttacataaag accgtctaat tcttcagttt caatggcatt
     7201 ttgagcaatt tgtttaagtc cttgttcatt tacttgacca ttttcatcaa aaggaacgag
     7261 taacgctgca tataaacctt ttaaatcttt gttcattatg aagtccctcc aaaaatcatt
     7321 tgataatata gtttacagct ataattgtaa acgctatcat aaaatgtaac aatatctttt
     7381 tgaaaattgt agtcatattt atgtataatt aatgaaaatg tttttcaaaa tcaatagaaa
     7441 tggagtgagt aaggtgtatt acatcgcaat cgatattgga ggcactcaaa ttaaatcggc
     7501 agttattgat aagcaattga atatgtttga ctatcaacaa atatcaacgc cggacaacaa
     7561 aagtgagctt attactgaca aagtatatga gattgtaaca ggatatatga agcaatatca
     7621 gttgatccaa cctgtcatag gtatttcatc agcaggcgtt gttgatgaac aaaaaggcga
     7681 aattgtatac gcagggccaa ccattccgaa ttataaaggt actaatttta agcgattatt
     7741 aaaatcactg tctccttatg tcaaagtaaa aaatgatgta aacgctgcat tactaggcga
     7801 attgaaatta catcaatatc aagcagaacg gatcttttgt atgacgcttg gtacaggcat
     7861 tgggggtgcg tacaagaata atcaaggtca tattgataat ggtgagcttc ataaggcaaa
     7921 tgaagttggg tatttattgt atcgtccaac tgaaaataca acgtttgagc aacgtgctgc
     7981 aacgagtgca ttgaaaaagc gcatgattgc cggaggattt acgagaagca cacatgtgcc
     8041 agtattgttt gaagcagctg aagaaggtga tgatattgca aaacaaatat tgaatgagtg
     8101 ggcagaagat gtagcagaag ggattgccca aatacaggtc atgtatgatc cagggcttat
     8161 attaattggg ggcggtatat ctgaacaagg agataatctc attaaatata tcgagccgaa
     8221 agttgcacac tatttaccaa aagactatgt ttatgcacca atacaaacga ctaagagtaa
     8281 aaatgatgca gcattatatg gctgtttgca atgatagttg aaagaaggag tcattctaaa
     8341 atagaatttg aaaccgttac gagagatgag agctgttgtt agttccacac atcacactct
     8401 atctaggacc aatctaaact atatcaacca acagtgtgcc acgggcaaat taaattgaag
     8461 aagctgagat attaaaattt tagaaaatgt aaaaaaatat ttggtattga aattaaaaaa
     8521 gcacctagca actcgttggg acaatcacga tgattgtcta cagttgcagg tggatttgaa
     8581 tatactacta gttatttgtt gtctaggata atagatttag tatgttgata agtttgactc
     8641 agattcgtat tttctaataa atgataactc acgatatcga ttaaaaagag tgtcgcaatt
     8701 tgtgtgttga taaattgatg gtcggtatta cacgattgat ccgttgttaa aagtactaaa
     8761 tctgcacaat ctgtaagttt actaccttca aaatttgtga tggcaacgac atatgcacca
     8821 tgagatttgg cgacttccgc tgcagaaatt aattccgaag tattaccact atttgacata
     8881 gcaataaaca tatccgaatg agatagtagg gatgccgata ttttcattaa atgtgaatcg
     8941 gtagtaacat taccttttag ccccatacga atcatacgat aataaaattc agtcgctgat
     9001 aaaccagagc tacctagtcc agcaaagagt atatgtcgac ttgattgaag tttgtcgata
     9061 aaggtttgga taatgtcgtt atcaataaat tcaccagttt gttgaatgat ttgttgatga
     9121 tatttatgaa ttctttgaat aattgggcta ttttcaataa ctgtctctgt catttcttgt
     9181 tgaatattaa attttaaatc ttggaaattc tcataatcca gcttatgact aaagcgtgtc
     9241 atcgttgctg gtgatgtacc aatcgcatgg gctaaggagt taatcgttga aaaggcatcg
     9301 ctataaccat tttgtcttat ataattgacg atgcgtttat cagtttttgt aaataaatgt
     9361 tgataacgtt gaacacgatt ctcaaatttc attgtgtcac cccttcatct taatgattac
     9421 tattatatat gaaaaatatt ttcaagatag taaaaagcat tgataaaaat tatcttaatg
     9481 atatattgta aatgacttta cgtgaaaaaa cgacttatgg agtgaggaat aatgttacca
     9541 catggattaa tagtatcttg tcaggcacta ccagatgaac cattgcattc atcttttatt
     9601 atgtcgaaaa tggcattagc tgcgtatgaa ggtggtgctg ttggtattcg cgcaaatact
     9661 aaggaagaca ttttagcaat taaagaaacg gtagatttac cagttattgg cattgtgaaa
     9721 cgtgactatg atcactcaga tgttttcatt actgcaacgt caaaagaagt tgatgaactg
     9781 atagaaagcc aatgtgaagt cattgcattg gatgcaacgt tacagcaacg tccgaaagaa
     9841 acgttagacg aattagtatc atatattaga acacatgcac cgaacgttga aatcatggct
     9901 gatatcgcga ccgttgaaga agctaaaaat gccgcacgac ttggctttga ttatattggc
     9961 acgacgttac atggctatac tagttatacg caaggacaat tactttatca aaatgacttc
    10021 caatttttaa aagatgtact acaaagtgtt gatgcaaaag ttattgcgga aggtaatgtc
    10081 attacaccgg atatgtataa acgtgtgatg gacttaggcg ttcattgttc agtcgttggt
    10141 ggtgcgataa cacgaccaaa agaaattacg aaacgttttg ttcaaattat ggaagattaa
    10201 atgataacga taaaaaaacg agatgaccat cattaattaa aggcacctaa ttatcttagg
    10261 tggctgaatg aatgtaatgg gttcatctcg ttttgtttgt ttatgatagt gattttattt
    10321 tcaactttat ccaaaaataa gtaaagcgac ggggatggtg attaatagcg acaacgccac
    10381 gcgtaaaaac caaatgatga tgagtttcca gacaggtatt ttaatttcag ttgctagtat
    10441 acatggcact aatgctgaga aaaagataat ggctgatacg cttactacac cgacgacaaa
    10501 tttagtactc attgcagctt tagttactaa caaagatggt agaaacatct ctacaataga
    10561 aatcgctgac gcttttgcta gtaaagcctg atcagcaatt gggaaaatat aaataaatgg
    10621 atagaagata tagccaagcc aatcaatgaa tggtgtatag ttcgctacaa tcagtcctaa
    10681 aaaaccaatc gataatatag aaggtaaaat accaacagtc atttctaaac cgtctttcaa
    10741 attgtcccaa acgttcttca cgagagatgg tgttaatgca ttttgtttca tcgcctctgc
    10801 atatgcagtt ttcagtctgc ttccttcaat agcaacttct tgttctcctt cttgtccgtt
    10861 ataatattct gttgattcat tgctgattgg cggtagccat gcagtaattg cagtcacgac
    10921 aaatgtgatg actaaagtta tccaaaagta taaattccaa tgcggcatta atcctaaagt
    10981 tttagcaacg ataatcataa aagttgctga aactgttgaa aagccagtcg caataatcgt
    11041 ggcttctcgt ttgttgtaca tcccttgctt atagacacga ttagtaatca ataatcctaa
    11101 ggaataactg ccgacaaacg aagccactgc atcgacagcg gattttcctg gtgttttaaa
    11161 aataggtctc ataataggct ccatataaac accgacaaat tctaataagc catagcccac
    11221 taataaagaa agcgcaattg cacctactgg aattaagata cttaatggca tcattaattt
    11281 ttcaaacaaa aacggaccat agttagcttt aaatagtatt gatggaccga ttttaaatac
    11341 atacattata ccgatcattg cacctgcaac tttaaataat gtaatgacca agtttgtgat
    11401 tgaagtcata aaagtacgtc tcactattgg taacgctgta ccaattaaaa tcataatcag
    11461 tgcaacatag ggcataagtg gacctatgat tgagcgaatg gctagatgaa catgatcgac
    11521 gaaaatagtg ttgttaccat taatcgtaaa aggaataaag aaacatagta tgcccactaa
    11581 actatagaca aaaaaacgcc atgcacttgg ttgttgtgca ttagaatgat attgattcat
    11641 taaagcaacc cctttgttta aatgaataca caaaactgta tgatgcatct tccccttaat
    11701 gagatgaatc attattttaa tttagaaaaa tctgaaaact tactataatt gtatagtttg
    11761 aattattttc ataccaatac aaattaacta attatatata gattgaaact atattactta
    11821 ataaaatatt tatcttaaat gttgttgtgt tgattcaaca ccacaactaa aagtgtttat
    11881 aaattatttg gaaatacaca tatttgtaaa tgattagtat cgatttaata tcgtattatt
    11941 aaatttttat taattttgta gtcttaatca aaaaataata tatgtcatgt tatattgaag
    12001 gtgcagttgt ttttcattct caagaggggg tcaaaaaaat acttttgagg tgattatatg
    12061 ttaagaggac aagaagaaag aaagtatagt attagaaagt attcaatagg cgtggtgtca
    12121 gtgttagcgg ctacaatgtt tgttgtgtca tcacatgaag cacaagcctc ggaaaaaaca
    12181 tcaactaatg cagcggcaca aaaagaaaca ctaaatcaac cgggagaaca agggaatgcg
    12241 ataacgtcac atcaaatgca gtcaggaaag caattagacg atatgcataa agagaatggt
    12301 aaaagtggaa cagtgacaga aggtaaagat acgcttcaat catcgaagca tcaatcaaca
    12361 caaaatagta aaacaatcag aatgcaaaat gataatcaag taaagcaaga ttctgaacga
    12421 caaggttcta aacagtcaca ccaaaataat gcgactaata atactgaacg tcaaaatgat
    12481 caggttcaaa atacccatca tgctgaacgt aatggatcac aatcgacaac gtcacaatcg
    12541 aatgatgttg ataaatcaca accatccatt ccggcacaaa aggtaatacc caatcatgat
    12601 aaagcagcac caacttcaac tacacccccg tctaatgata aaactgcacc taaatcaaca
    12661 aaagcacaag atgcaaccac ggacaaacat ccaaatcaac aagatacaca tcaacctgcg
    12721 catcaaatca tagatgcaaa gcaagatgat actgttcgcc aaagtgaaca gaaaccacaa
    12781 gttggcgatt taagtaaaca tatcgatggt caaaattccc cagagaaacc gacagataaa
    12841 aatactgata ataaacaact aatcaaagat gcgcttcaag cgcctaaaac acgttcgact
    12901 acaaatgcag cagcagatgc taaaaaggtt cgaccactta aagcgaatca agtacaacca
    12961 cttaacaaat atccagttgt ttttgtacat ggatttttag gattagtagg cgataatgca
    13021 cctgctttat atccaaatta ttggggtgga aataaattta aagttatcga agaattgaga
    13081 aagcaaggct ataatgtaca tcaagcaagt gtaagtgcat ttggtagtaa ctatgatcgc
    13141 gctgtagaac tttattatta cattaaaggt ggtcgcgtag attatggcgc agcacatgca
    13201 gctaaatacg gacatgagcg ctatggtaag acttataaag gaatcatgcc taattgggaa
    13261 cctggtaaaa aggtacatct tgtagggcat agtatgggtg gtcaaacaat tcgtttaatg
    13321 gaagagtttt taagaaatgg taacaaagaa gaaattgcct atcataaagc gcatggtgga
    13381 gaaatatcac cattattcac tggtggtcat aacaatatgg ttgcatcaat cacaacatta
    13441 gcaacaccac ataatggttc acaagcagct gataagtttg gaaatacaga agctgttaga
    13501 aaaatcatgt tcgctttaaa tcgatttatg ggtaacaagt attcgaatat cgatttagga
    13561 ttaacgcaat ggggctttaa acaattacca aatgagagtt acattgacta tataaaacgc
    13621 gttagtaaaa gcaaaatttg gacatcagac gacaatgctg cctatgattt aacgttagat
    13681 ggctctgcaa aattgaacaa catgacaagt atgaatccta atattacgta tacgacttat
    13741 acaggtgtat catctcatac tggtccatta ggttatgaaa atcctgattt aggtacattt
    13801 ttcttaatgg ctacaacgag tagaattatt ggtcatgatg caagagaaga atggcgtaaa
    13861 aatgatggtg tcgtaccagt gatttcgtca ttacatccgt ccaatcaacc atttgttaat
    13921 gttacgaatg atgaacctgc cacacgcaga ggtatctggc aagttaaacc aatcatacaa
    13981 ggatgggatc atgtcgattt tatcggtgtg gacttcctgg atttcaaacg taaaggtgca
    14041 gaacttgcca acttctatac aggtattata aatgacttgt tgcgtgttga agcgactgaa
    14101 agtaaaggaa cacaattgaa agcaagttaa attcatcttc tgaatttaat atgctatgta
    14161 aatcgtgctg ttatcacggc acatcagata taagtagcat cacagtgttg aatttaaaaa
    14221 tagtaaagtg aaataaagcg cctgtctcat tagcgaaaac taaagggaca ggcgtatctg
    14281 tttatgagct taataaattg tatgaataat atggttgatc gaataactgt ttatcatgat
    14341 gataaattga gttttttaaa ataatgatat attacatcat tgttatagcg tttaagaaat
    14401 caacaacttt acgataaata gtgattgctt cgtcattagg tctacgatca aaatcatgct
    14461 cgtttttatt cacgcgttca aatgttgaat gtggaacatg attcatgata tgttcgcttt
    14521 cctcaacggg aacatcataa tcgccattac aatgcgcaat gaaaacaggt ggaagtgttt
    14581 taagttcatc tggtgcaata ttatattttg aattagtata atcagcaatg ttaatcatat
    14641 ttatccattt acctgtgcca cgtgcataaa cgtagattaa aaaacgttgt gcgatttgat
    14701 cttgaacaac cggtgttggt gaagtgagtt gtgcaatcat tgtttcgttt acgctttgag
    14761 ctatttttgc gtaataacta ttagttgttt taaaaggttc agtgttgatg cgactataac
    14821 cataaaaatc aataacacca tcaatatctc tgtctcgtgc aattaataga cttaaatatg
    14881 cacctgatga tctgccaaag gtaaaaatag ggcaattaga atattgtgat tgaatcgcat
    14941 cgaatgatgc gtagacatcc tcaataatgc aatcgagact tacttctggt aataaacgat
    15001 aacttagttg aattaaatcg taatgttccg taaggatatc gatatactgt ggggataaat
    15061 cgttagcttt accgaacatt aatccaccac cgtggatgta gacaatagcg ccttttgttg
    15121 gttgattttt tgctttaata attgtgtaag gtaatgcaaa tgcatcttta gtaattactt
    15181 tatctttaat ttcagtcacg atttaatagg ctccttattt ttgatattga tgtcattata
    15241 acactgtctt aaatttccat gaaaaatagt cttaagacga tgagtcatga taattctgtt
    15301 ccaattgacg taaagcgtca cgggtatgct tctttagacc ttccccataa tccatcattt
    15361 taacaatatc tttaaaagca gcatgtggaa tggctaaatc ttctaaatct gccatagaaa
    15421 attcaagatt gatatcatgt ggtcgctgtt cagcaagttt atgcacaaag tcaggttctg
    15481 tgacaaaagg cgaagacatg ccgaccatat ctgcatgttg taaagcatct aaagcagact
    15541 ctggagaatt aatcccgcca cttgcaatta aagggatacg acctgctaaa tgttcataga
    15601 caatttggtt aactggtcga ccgaaatgat cacctggtgt acgagacgta ttttgataaa
    15661 tatgtcgacc ccagctagcg attgctaagt attggatgtt tgaaacgtcc atgacccaat
    15721 tgattaattg gttgaactcg tcaatggtat atcctaaatc actgcctctg gtttcttctg
    15781 gcgttgctcg aaatcctaaa ataaaattgt caggtgcttc tttatcaatc acttcttgta
    15841 ccgcacgcat aacttctaaa cataatcttg cacgattttt taatgagtcg gcaccgtaat
    15901 ggtctgtacg tttattcgaa aaagttgaga aaaatgtttg aatcagcaaa cgttgtgcaa
    15961 tcgaaatttc cacaccatca aaacctgctt taatcgcgcg taatgtagca tcgcgatact
    16021 gctgaatgat gctattgatt ttctcatgag acatggcgat aacatcgtgt tcaatcggtg
    16081 aatgcaatgt catagggctt ggtccataca cctttccaaa atttaaaatg gcttgatttg
    16141 aaaaacgacc agcatgcgct agctggataa tagcgaggct accatgttgt ttcatcgtag
    16201 atgccatgtt agttaatcca gggatacaag catcatgatc aatattaaag ccatattcaa
    16261 acaattgacc ataaggttca atgtaagcag cgccggtgac ttgcattcca gctgaattag
    16321 agcgacgtgc agcataagcc aagtcttctt ttgtaatata gccttctttt gttgatgtgt
    16381 ttacggtcat tggtgataat acaaagcgat tcgaaatttt gatgccatta ggtaagtgga
    16441 ttgattgtaa aagtggtttg tatcggtaca tactatgatt ccttttctat tcaatattgt
    16501 tttcaaagta ccatggaaag aatgaataat caatgatgaa cagtcttgat agaatagaat
    16561 tggtacatgg aaagtatttt taaaattaaa ctaatgaatg gcatttgtag gtctgaaaat
    16621 atgaatatga aaaagaaaaa taaaggcgaa aagatataaa agttaattga aaaacgttat
    16681 catatacgtg ggtatatgaa gagggaatgg tattaagaac gctaaaatgt tatgtcggtt
    16741 tgacatgaca ggataagttt ggagatgacg gattggttaa attaagcgta ttagactatg
    16801 ccttaataga tgaaggtaag gatgcacaaa aggcattgca agattcagtg acacttgcaa
    16861 aattagcaga tcgacttggc tttaagcgaa tttggtttac ggaacatcat aatgtaccag
    16921 cgtttgcgtg tagtagtcca gaacttttga tgatgcatac attggcgcag acaaatcaca
    16981 tacgagttgg ctctggtggt gtgatgctgc cgcactatcg accttataaa attgctgagc
    17041 attttagaat gatggcagcg ttatatccaa atcgtattga tttaggtatt ggtaataatc
    17101 caggtactac tatggtaaag caagctttag atggaataaa tcctacatat gatagttacg
    17161 atgaatcgat ttcgttatta cgtgattatc ttacaataaa ggataaacca agtgcgcata
    17221 cgttaggtgt ccaaccacac attgatcatt ttccagaaat gtggttatta agtagtagcg
    17281 caacatctgc caaaatagct gccgaactag gtatagggct ttctgttgga acatttttgc
    17341 taccagatat aaatgcgata catgcagcga aggataacat tgatatttac aaaaaacatt
    17401 tccaagcatc aacgattaaa atggacgcaa aggtgatggc atctgtattt gtcattgtag
    17461 ctgataacga agcggaagta gcagcattac aacatgcctt agatgtttgg ttattaggta
    17521 aattacaatt tgcagaattt gaagattttc cttcagtaga cacagcacaa aagtataagc
    17581 ttaatgatcg agacaaagag atgattcaag cacatcaagc acgcatcatt gcaggtacac
    17641 aagaaaaggt taaagcacaa ttagatgatt tcattgctac gtttgaagtt gatgaggtgt
    17701 tagtagcacc gcttattcca ggtattgaac agcgttgtaa aacattaaaa ttactcgcgg
    17761 aaatttattt gtagcatttt aaatagaaga gaaaggatga agataagatg aaaaagttag
    17821 ccaattattt atgggtagaa aaagtaggag atttgtatgt gtttagtatg acacctgaat
    17881 tgcaagatga tattgggaca gtaggttatg ttgaattcgt aagtccagat gaagttaaag
    17941 tggatgatga aattgtgagt atcgaagcat cgaaaacggt cattgatgtg caaacgccat
    18001 tgtcaggaac gattattgag cgaaatacaa aagcggaaga agaaccgaca attttaaact
    18061 ctgaaaaacc agaagaaaat tggttgttca aattggatga tgtcgataaa gaagcattcc
    18121 tagcattacc ggaggcttaa atggaaacgt taaaatcaaa taaagcgaga cttgaatatt
    18181 taatcaatga tatgcatcga gagagaaatg acaatgacgt attggtaatg ccatcttcat
    18241 ttgaagattt gtgggaatta tatcgaggct tagcaaatgt cagaccggca ttacctgtaa
    18301 gtgatgaata tttagctgta caagatgcta tgttaagtga tttgaatcgt caacatgtta
    18361 cggatttgaa ggatttgaag ccgataaaag gtgacaatat ctttgtttgg caaggtgata
    18421 tcacgacgtt aaaaatcgat gctattgtta atgctgcaaa tagtcgtttt ctaggatgta
    18481 tgcaagctaa tcatgactgc attgataata ttattcatac aaaagcgggt gttcaagttc
    18541 gacttgattg tgcagagatc attcgacaac aagggcgcaa tgaaggtgta ggtaaagcca
    18601 aaataacacg tggatataat ttgtcagcaa agtatataat tcatacggtt ggtccgcaaa
    18661 tacgtcgatt gcctgtttca aagatgaatc aggacttgtt agctaaatgt tatcttagct
    18721 gtcttaaatt ggctgatcaa catagtttaa atcatgtcgc tttttgctgt atatctacag
    18781 gtgtatttgc ttttcctcaa gatgaagcag cagaaattgc tgttcgaaca gtagaaagct
    18841 atctcaaaga aacaaattca acattgaaag tcgtgttcaa tgtatttaca gataaggatt
    18901 tacaactgta taaggaggca tttaaccgtg atgcagagta gtaagtggaa tgcaatgtct
    18961 ctgttaatgg atgacaagac aaagcaggct gaagtattgc gtactgcgat tgatgaagca
    19021 gatgcgatag tgattggaat tggtgcaggc atgtctgcat ctgacggatt tacatatgta
    19081 ggagagcgtt ttacggaaaa tttcccagat tttattgaaa aatatcgctt ctttgatatg
    19141 ttgcaagcga gtttacatcc ttatggcagt tggcaagagt attgggcatt tgagagtcgt
    19201 tttattacat taaactattt agatcaacct gtaggtcagt cttacctcgc tttaaaatcc
    19261 ttggtggaag gtaaacagta ccacattata actacgaatg cagataatgc tttcgatgta
    19321 gctgattatg atatgactca tgtatttcat atacaagggg agtatatact gcaacagtgt
    19381 agtcagcatt gtcatgctca aacgtatcgc aatgatgatt taattcgtaa aatggttgtt
    19441 gcgcaacaag atatgcttat accttgggag atgattccaa gatgtccaaa atgtgatgcc
    19501 ccaatggaag tgaataaacg taaagcggaa gttgggatgg ttgaagatgc tgaatttcat
    19561 gcgcaactac atcgttataa tgcttttcta gagcaacatc aagatgataa agtgttgtat
    19621 ttggaaattg gaattggtta tactacacca caatttgtga agcatccttt tcagcgtatg
    19681 acacgtaaaa atgaaaatgc cctttatatg acgatgaata aaaaggcata tcgcattccg
    19741 aattcaattc aagaacgtac catacattta actgaggata tctcaacatt gattacagca
    19801 gcactccgga acgacagcac aacgaaaaat aacaacattg gagagacaga agatgtactt
    19861 aatagaaccg attagaaatg gagaatatat tactgatggt gcgattgcac tcgctatgca
    19921 agtttatgtt aaccagcata tctttttaga tgaagatatt ttattccctt attattgtga
    19981 tccaaaagtg gaaattggac gttttcaaaa tactgctata gaagtgaatc aagattatat
    20041 agataaacac agtattcaag tagttcgccg agatactggt ggtggcgctg tgtatgttga
    20101 taaaggtgcc gttaatatgt gttgtatttt agaacaagac acttcaattt atggtgattt
    20161 tcaacgattt tatcaaccag ctataaaggc gttgcataca ttaggtgcaa cagatgtggt
    20221 acaaagcggt agaaatgatt taacattgaa tggtaaaaaa gtgtcaggcg ccgcaatgac
    20281 attaatgaat aatcgtattt atggcggtta ttcgctatta cttgatgtta attatgaagc
    20341 aatggataaa gtgttaaagc ctaatcgcaa aaagattgca tcgaaaggga ttaaatctgt
    20401 gcgcgcacgt gttggtcatc ttagagaagc actggatgaa aagtatcgtg atataaccat
    20461 tgaagaattt aaaaatttaa tggtgacgca gattttggga atcgatgaca ttaaagaggc
    20521 gaaacgatat gaattaacgg atgcagattg ggaagcgatt gatgaattag ctgataaaaa
    20581 gtataaaaat tgggattgga attatggcaa gtcacccaaa tatgaataca atcgaagtga
    20641 aagattatct tcaggtacgg tagacataac aatttctgtt gaacaaaatc gtatcgcaga
    20701 ttgtcgtatt tatggggatt tctttggaca aggtgatata aaagatgtgg aagaagcatt
    20761 acaaggaaca aaaatgacaa gagaagattt aacgcatcag ttaaagcaat tagacatcgt
    20821 ttattatttt agcaatgtta cggtagaagc attagtggat atgattttaa gttaatattg
    20881 ttattttatg tatgctgaat cattggaagt gtttgcttgc tcttgaaaag gtgacaatag
    20941 tgtttggtga aggttgaaca tatgagtgga aattattgcc tttaactatt caaagtatga
    21001 tatatatatg gtttttgttt ctaaatgatt gggtatttga aaatagatga gtttaatatt
    21061 ttaaggaata taatgatgtt tacttttata attcatatag aatattaagc aatataagtc
    21121 tgttgatata tacaaaatat aatgactgct ataatgagta atcaatagac acaaagagga
    21181 gattatgtga tgaataataa agtattagca accggtggta cagggtttgt tggcatgcga
    21241 attatttcac gattattaga acaaggttat gacgtacaaa cgacgatacg tgatttaagt
    21301 aaagctgata aagtaattaa aacaatgcaa gacaatggca tttccacaga gcgattaatg
    21361 tttgtcgaag cggatttatc acaagatgaa cattgggatg aagcaatgaa agattgcaag
    21421 tatgtcttga gtgtagcatc tccggtgttt ttcggtaaaa cagacgatgc agaagtgatg
    21481 gcgaagcctg caattgaagg tatacaacgt attttaagag ctgcagaaca tgcgggtgtt
    21541 aaacgtgtgg taatgactgc aaactttggt gcagttggtt ttagtaataa agataaaaat
    21601 tcaatcacaa atgaaagtca ttggacaaat gaagatgaac caggcttatc agtatatgaa
    21661 aaatcaaaat tgttagctga aaaggcagcg tgggattttg ttgagaatga aaatacaaca
    21721 gtagaatttg ccacaatcaa tccagttgca atttttgggc catcattaga tgcacacgtt
    21781 tcaggaagct ttcatttatt agaaaattta ttgaatggtt caatgaaacg tgtaccgcaa
    21841 attccgttaa atgttgttga tgtgagagac gtagctgaac tgcacatttt ggcaatgaca
    21901 aatgaacaag ctaatggcaa gcgatttatt gcgacggctg atggacaaat taatttgttg
    21961 gaaattgcaa aattaattaa agaaaagaga cctgaaatag ctcaaaaagt ttctactaaa
    22021 aaattaccag actttatttt gagtctaggt gctaaattta atcatcaagc taaagaaggt
    22081 aaacttttat tagatatgaa ccgcaatgta agtaacgaac gtgcaaaaac attacttggt
    22141 tgggaaccga ttgcgacaca agaagaagca attttagcag ctgtcgatag tatggctaag
    22201 tatcatttaa tataatacaa aacaccgtcc ataagtaagt ggctttcctc ttgataaaag
    22261 gggcattcac tcaatatgtg acggtgtttt atatttctac gttaacacta ttgctgttct
    22321 ttttgacggc cttttaataa aattgttgtc gcacctacga taataataaa tagaatcgca
    22381 ccaaataatc ccatatattt tactgcgtta ccgaacacga taccgacagc taaaaagtct
    22441 gtatctgaga atgttgttgc agcaccacct aattcgccta aaaatggcaa gaataataat
    22501 ggtaaaaacg tgattaggat accatttaga gcggcgccag caatagcacc tttaataccg
    22561 cctcttgcat taccgaatac agcagccgtt gcacctaaga agaagtgtgc aactacgcca
    22621 ggtaaaatga cgacgccacc aaataagaat aagataaaca taccgatgac acctgtaata
    22681 aagctgacaa agaatccaat taatactgca ttttgtgcat aagggaacac aatagggcag
    22741 tctaatgcag gtttagaatt tggtacaagc ttttcagaaa ttcctttaaa tgctgggacg
    22801 atttcagcta agattaaacg aacgcccgtt aaaataataa atacaccagc agcaaatgtc
    22861 acaccttgaa ttaatgaaaa gacaataaag ttttgaccat cactaataga ttcgtgtaca
    22921 taactaacgc ccgcaaataa gcatgcgatg aagtaaagta atgccatcgt aatcgagata
    22981 ctaattgtac tttctcgtaa gaaacttaag ccttttggaa atttaatctc ttccgttgat
    23041 ttagacttac ctttgaataa ttgacctaca gcacctgcgg caaagtaact gattgagcca
    23101 aaatgaccta aagctacttg gtcattccct gtaatttttc gcatcgtagg ttggagtaat
    23161 gcaggtaata ctgccatgat taatcctaat acgagtgcgc cgataacaat cgttagccag
    23221 cctttaatat gactgactgt taaaatgatt gctaaaaacg cagccatgta aaatgtatga
    23281 tgacctgtta aaaagatata ttttaaatta gtgaagcggg caattaaaat attaacaatc
    23341 atgccacaga ccatgatgag tgcagctgtt gttccaaaat cttttaaggc tagtgagacg
    23401 atagcttcgt tgttaggtac gataccttgc acaccaaatg cgtgttggaa tattttgccg
    23461 aatggttcaa gagatcgaac gacgacatca gcacctgcac ttaaaattaa gaagcctaat
    23521 atcgttttaa tggttcctga agtgatcgtt gcggcaggtt ttttctgaac gattaaacct
    23581 ataaaggcaa tcagtgcaac aagaatggct ggttgactta aaatatcgac tataaaatta
    23641 aggattgctt gcataggtcg tacctccttt aaatcatgtt aagttgttgt aatttttctg
    23701 agagcttttg ttgtaattca gctttatcta aaatattatc aagaactaag acatccccta
    23761 gacgttcggc attttcagct aaatctctac cacaaataaa caagtcagcc atctctggac
    23821 ttgctgtcat aatgtcacta tgttcaactt cgatatcaga tggtgcatta agttgcctaa
    23881 gtgcttcttg tgcgttcatt tctaccataa aactacttcc taaaccgtgg ccacatacta
    23941 ctaaaatttt catattaatc atgctccttt aaaatgtttt taatgtcttg tgcatttgtt
    24001 gcagttaata gttgctggac tgtttggtta tcgcccagta cggttgctaa attttgtaat
    24061 acagataagt gtgaatgatt gtcgatggca ctcaatacaa aaatgagaga tgcgtagtga
    24121 tcttcatcac aaaatgccac atgttgattc aactttaata gacttaaacc aacttgatgt
    24181 acgtcattgt tcggtcttgc atgtgcaatt gcaatttcag gtgcgataac gatataaggt
    24241 ccaagttcat taacgctatc aatcattgct tgaacatagc cttgttcaat aatttgttct
    24301 tgtagtaatg gctgagaagc tatagttata gcttcagtcc aatcatttac ttgttctttt
    24361 acaatgatgc gtgttgttga caaaatgtct aatgacacgt tattaagcct cctttgtcat
    24421 agttaaagca atgtgttgtt taattttaaa aatattccca tctaagaaat cttgtcgata
    24481 taagtcgttg cttaagcatt cgcttaactg tcccaatgcc tttaaatgtg cattggggtg
    24541 gtccgttgct aatgtaatta caaggtgaac gggatcgtta gctttactac caaagataat
    24601 cccttcagtg aaatatgtta gtgcgaaacc tacaccattc tgtacataat cagtaccagc
    24661 gtgaataagt gcaatatgtg gactaatgac catatatgac ccgaattgtt caaattgttt
    24721 taaaattgca gctgtataat ttgaatagac aatgccatca ttgattaaag gttgcacagc
    24781 cactgcaatt gcggattcaa ttgataatgg ttgtttattt ataatgatgc gatgttcagg
    24841 caataaatct gcgagtgact tgccatcagt tgccattttc atgactcgtt gttctcttga
    24901 gtcattgata atttgattca atttttgacg agattgttga ttgataaatg gatcgacatg
    24961 aataactggt acagctgata tttcacaagg tactgttgaa atgacataat caatgttatc
    25021 ttgcaataat cgactttctt ccaattgata aatggaatag gcatcccaaa tgtgaaactc
    25081 aggatacagg tgatttagtt ttgattttaa aagttgtgac gtgcctatac cagaaccaca
    25141 tagtaagaca accttaatca ttgattgttt atgtgttgca acacgctcta tacttgatgc
    25201 gaagtgaatt gtaatgtatg ttaattcatc ttcgttgaag cgaatagcag catcttgttc
    25261 aattggacta atatgcttgc taacggcttc aatgatttga ggatagcgac gcataacttc
    25321 ttgcctcaaa ggattaggtt gtagcatatc gtatttaata cgatgtatag ctggtttgat
    25381 atgtgtgatc agactggtat gtaacttgtt gtcttttgac atatcaatgc ctaattcttg
    25441 gctaacacaa gtgatcaatt catgtatatt ttgcgataaa tcatggtatt caaaggtaat
    25501 tgaagatgct gtatgttcag tcattttaga gcctagtaaa tgtaacgtga taaagataat
    25561 ttcagactct ggaaatgtga cattacaact gcgttctaag ttttctatca tttttgaagc
    25621 aatagcatac tgattagtat gtcgccattt atcaatttca ttgataggta tatcgaacga
    25681 aaaattttca tttaaacgct gaatggcaat gagtatatga tagattaagc catcgatagc
    25741 cgactgaact aaatgataat tttcactatt taatgtctta ataatggcac ggcgaaccaa
    25801 tgcgattgat tctgaattaa agatatccgc ctctataaaa ggtgcagctt gtttcatata
    25861 ttgatgtata aagtgtgcat acgctttacg ataatgatct tcctcaccaa taatattgaa
    25921 tcctttattg tggacataat ttaactttaa atggtattga tctagttggg cttgaatcat
    25981 tttaatatca tctgcaattg tccgacgcga aacattaaca tcttgcgcaa gttgctttgt
    26041 tgaaacagga tcggttgttt cgaataactt taaagcgata tgtgtgagtc gttcatcttt
    26101 tgaaaaatga atttgatttg ttagattgtg ctctaattca tttaatagtg tcgcgtgagc
    26161 tgttgttact ttgatgcctg cagctttatt acggctgact tggtaatgat aagtttcagc
    26221 atattgctca atatatgcta tatcatattg aatggtacga ggtgatacac caagttgatt
    26281 agcaatggta ttgattggaa taaacgtttg ctcatgaatt aaaagataca aaatttcgat
    26341 ttgtctataa cttaacaacg taatatcctc ctatttgtaa ttgtaagcga tttcttaaaa
    26401 acgtagatat gcaatctctt tcatatttta atccgaaaaa ttgcatatca aaatgtttat
    26461 ggcgcaagat tttataggaa cttttaaaat aaattagata ttcatgttgt caatttaaaa
    26521 atgtcgcagt atatttagtt agacatctaa cgaaatggtg gtgcaataaa tggaattcac
    26581 ttattcgtat ttatttagaa tgattagtca tgagatgaaa caaaaggctg atcaaaagtt
    26641 agagcaattt gatattacaa atgagcaagg tcatacgtta ggttatcttt atgcacatca
    26701 acaagatgga ctgacacaaa atgatattgc taaagcatta caacgaacag gtccaactgt
    26761 cagtaattta ttaaggaacc ttgaacgtaa aaagctgatc tatcgctatg tcgatgcaca
    26821 agatacgaga agaaagaata tagggctgac tacctctggg attaaactcg tagaagcatt
    26881 cacttcgata tttgatgaaa tggaacaaac actcgtatcg cagttatctg aagaagaaaa
    26941 tgaacaaatg aaagcaaact taactaaaat gttatctagt ttacaataaa tgataagtgt
    27001 gactggtaga aatcagtcac tttgtcttta atattatagt tagatatcta attgttagta
    27061 agctaattat tggaaaagac aaggagtatt gaacaatgaa agacgaacaa ttatattatt
    27121 ttgagaaatc gccagtattt aaagcgatga tgcatttctc attgccaatg atgataggga
    27181 ctttattaag cgttatttat ggcatattaa atatttactt tataggattt ttagaagata
    27241 gccacatgat ttctgctatc tctctaacac tgccagtatt tgctatctta atggggttag
    27301 gtaatttatt tggcgttggt gcaggaactt atatttcacg tttattaggt gcgaaagact
    27361 atagtaagag taaatttgta agtagtttct ctatttatgg tggtattgca ctaggactta
    27421 tcgtgatttt agttacttta ccattcagtg atcaaatcgc agcaatttta ggggcgagag
    27481 gtgaaacgtt agctttaaca agtaattatt tgaaagtaat gtttttaagt gcaccttttg
    27541 taattttgtt cttcatatta gaacaatttg cacgtgcaat tggggcacca atggtttcta
    27601 tgattggtat gttagctagt gtaggcttaa atattatttt agatccaatt ttaatttttg
    27661 gttttgattt aaacgttgtt ggtgcagctt tgggtactgc aatcagtaat gttgctgctg
    27721 ctctgttctt tatcatttat tttatgaaaa atagtgacgt tgtgtcagtt aatattaaac
    27781 ttgcgaaacc taataaagaa atgctttctg aaatctttaa aatcggtatt cctgcatttt
    27841 taatgagtat cttaatggga ttcacaggat tagttttaaa tttattttta gcacattatg
    27901 gaaacttcgc gattgcaagt tatggtatct catttagact tgtgcaattt ccagaactta
    27961 ttatcatggg attatgtgaa ggtgttgtac cactaattgc atataacttt atggcaaata
    28021 aaggccgtat gaaagacgtt atcaaagcag ttatcatgtc tatcggcgtt atctttgttg
    28081 tatgtatgag tgctgtattt acaattggac atcatatggt cggactattt actactgatc
    28141 aagccattgt tgagatggcg acatttattt tgaaagtaac aatggcatca ttattattaa
    28201 atggtatagg tttcttgttt actggtatgc ttcaagcgac tgggcaaggt cgtggtgcta
    28261 caattatggc cattttacaa ggtgcaatta tcattccagt attatttatt atgaatgctt
    28321 tgtttggact aacaggtgtc atttggtcat tattaattgc tgagtcactt tgtgctttag
    28381 cagcaatgtt aatcgtctat ttattacgtg atcgtttgac agttgataca tctgaattaa
    28441 tagaaggtta aatatttcgt ccacttctgg ctgagtatat ttcggtcgga agtgtatttt
    28501 tcgaaaaaaa taaatatatg atacgattat gaaaaaataa agtgagattg gcatatgtgt
    28561 aaatctaaaa tactgttgaa aaatattttt agtgaagaat cagaagttaa agatttaact
    28621 gaagaaaaat ataatcaaga ttacgaagca ttaacattta gctttaaaga ggaaacatat
    28681 caaagtaggt tagctaagaa aacaccgact aaatcgggat atttcgtgac atgttggaca
    28741 aaagacgaag ataattataa tcggccatac aaaattgaag agtttgctga ttacctgatt
    28801 gttgctgtta tcgatgatga attaaatggc tactttctat ttcctaggga attattggta
    28861 gaaaaaggta tcttagcttc atctaagtat caagggaaaa tggcttttag agtttatcct
    28921 aagtggtgta atcaattgaa taaaacagca gggcaaacac aaaagtggca atgtaaatat
    28981 ttttttgaat actaataaaa agtcatatgg ttgaacttat aagagatgaa tatatcatct
    29041 ttcgataaga taaccatatg actttatttt ttatcattta atgatgaacg gtttcttgtc
    29101 ctactttatt ccaagtgagg ataaagctca acattgcaaa cacactaatt gctgttaata
    29161 aaataaaacc gacatcccat ccgaatttat caactacagc acctaagacg atgttggcca
    29221 ttacagcacc aaacagataa ccaaataatc ctgttaatcc agctgctgtg ccagctgctt
    29281 ttttaggtac ataatctaat gcttgtaaac caattaacat aactggtcca tatattaaga
    29341 aaccaatggc aattaatgag acattgtcta accaagcatt gcctggagga tttaaccaat
    29401 aaattaatac aaatactgtg acacctaaca taaagaagaa acctgcaggt ccacgacgac
    29461 ctttgaataa tttatcagaa atgtaaccac ataataatgt accaggaatt ccagcccatt
    29521 cgtataagaa gtatgcccaa cctgatgctt ttaagtcgaa atgtttttct tcacttaagt
    29581 agactggcgc ccaatcaagt acaccataac gcacgaaata aacaaatata tttgcaaagg
    29641 caattgccca tacccattta ttgttcagta catatttaaa taaaatttct tttgtagtta
    29701 attctgtttc taatgttttc ttatcgcttg tagcaaagtc atttttataa atttcgattg
    29761 gaggtaaacc ttgagattga ggtgtgtctc taatcaatac gtatgaaatt gcggcaatga
    29821 taagtgctaa gagtgcaggg taaatgaata caccttcgaa accttttaaa taaccaaagt
    29881 tgataaatgc agttgttgta ataccccaag cagcaatagg tgccataata cctccaccaa
    29941 cattatgcgc aacgttccaa agggcagtct tacttccgcg ttcacttaca ctaaaccagt
    30001 gaacgagaac acggcctgaa ggtggccagc ccataccttg aaaccatcca tttaagaata
    30061 ataggacaaa cataataccg atacctgatg tgaagaacgg tacaaatccc attaacaaat
    30121 tgacgatagc agtgagtgct aatccaagaa ctaagaatat ccgagcattg ctccgatcac
    30181 ttacagtacc cataaagaac ttactaaatc catatgcgat ggaaacagca gaaagtgcaa
    30241 aacctagttc cgcttttgta aaaccttgct cttgcaatgc cggcatcgct aacgaaaagt
    30301 ttttacgtaa taaatagtac ccagcataac cgatgaaaat accaagaaat acttggagac
    30361 gtaatcgttt ataggtatca tctatctgat tttctggcaa aggcttaata tgctttgcag
    30421 gtttaagaaa attcataaaa tcctccttaa tatgtattta tatgcatttt gtgcgataaa
    30481 cttatattag acatgtatga aagcgttgtc aatatgcttt tgtgaaa
//
LOCUS       DAKTIO010000013        29386 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.013, whole genome shotgun sequence.
ACCESSION   DAKTIO010000013 DAKTIO010000000
VERSION     DAKTIO010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 29386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29386
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.013"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..1007)
                     /locus_tag="O0293_000444"
     CDS             complement(<1..1007)
                     /locus_tag="O0293_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HCY0279354.1"
                     /translation="MKLQCINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSF
                     QFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIGYSDTIVMQVYLAERFGNIIDVL
                     EETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
                     SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQ
                     LFKTYFVTNELVLFYKNGITLQSIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQIL
                     EKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIKQTQFLQPILFLILG
                     L"
     gene            complement(979..1953)
                     /locus_tag="O0293_000445"
     CDS             complement(979..1953)
                     /locus_tag="O0293_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HCY0279355.1"
                     /translation="MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQ
                     IGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYLFNKIYFLRISTLPLSLGQESCVI
                     RIVPQFFQPQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
                     VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVI
                     QASLSGHLVLTTLHATDCKGAILRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLV
                     CEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTMHKYI"
     gene            complement(2005..2628)
                     /locus_tag="O0293_000446"
     CDS             complement(2005..2628)
                     /locus_tag="O0293_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCY0279356.1"
                     /translation="MRISSLTLGLVDTNTYFIENDKAVILIDPSGESEKIIKKLNQIN
                     KPLKAILLTHAHFDHIGAVDDIVDRFDVPVYMHEAEFDFLKDPVKNGADKFKQYGLPI
                     ITSKVTPEKLNEGSTEIEGFKFNVLHTPGHSPGSLTYVFDEFAVVGDTLFNNGIGRTD
                     LYKGDYETLVDSIQDKIFELEGDLPLFPGHGPYTTVDDEQLNPFLHG"
     gene            complement(2625..2954)
                     /locus_tag="O0293_000447"
     CDS             complement(2625..2954)
                     /locus_tag="O0293_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HCY0279357.1"
                     /translation="MAIVDVVVIPVGTEGPSVSKYIADIQKKLQEYKAMGKIDFQLTP
                     MNTLIEGELSDVLEVVQVIHELPFDKGLSRVCTNIRIDDRRDKSRKMNDKLTSVQKHL
                     ENSGENL"
     gene            complement(2954..3940)
                     /locus_tag="O0293_000448"
     CDS             complement(2954..3940)
                     /locus_tag="O0293_000448"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HCY0279358.1"
                     /translation="MTKIILAADVGGTTCKLGIFTPELEQLHKWSIHTDTSDSTGYTL
                     LKGIYDSFVEKVNENNYNFSNVLGVGIGVPGPVDFEKGTVNGAVNLYWPEKVNVREIF
                     EQFVDCPVYVDNDANIAALGEKHKGAGEGADDVVAITLGTGLGGGIISNGEIVHGHNG
                     SGAEIGHFRADFDQRFKCNCGRSGCIETVASATGVVNLVNFYYPKLTFRSSILELIKE
                     NKVTAKAVFDAAKAGDQFCIFITEKVANYIGYLCSIISVTSNPKYIVLGGGMSTAGPI
                     LIENIKTEYHNLTFAPAQFETEIVQAKLGNDAGITGAAGLIKTYVLDKEGVK"
     gene            complement(3937..4140)
                     /locus_tag="O0293_000449"
     CDS             complement(3937..4140)
                     /locus_tag="O0293_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HCY0279359.1"
                     /translation="MSRKINNFYDVQQLLKSYGFLIYFKNPEDMYEMIQQEISSLYQY
                     ELLSKEEYLKCTLIINQRRNEQK"
     gene            complement(4121..5584)
                     /locus_tag="O0293_000450"
     CDS             complement(4121..5584)
                     /locus_tag="O0293_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCY0279360.1"
                     /translation="MNIDKQFWKTIYYWIRYLNFDIVSREKDDQEIWLAHKRKKQVVI
                     FKQHIKSTQEIRFDKAKVLEHKDEIANFISFEPQSFEFYYFTESEFSEEQLNEVSPIR
                     IKFNVIRHTKDLIKHMPNIFLARLISEDNDKKTYMFYKRKVLTDNFLDKYMQKFSPAT
                     YTIIFVNVLIWLCMILYLNNFSDVKLLDVGGLVHFNVVHGEWYRIVTSMFLHFSFEHI
                     LMNMLSLFIFGKIVEAIIGSWRMLTVYFIAGLFGNFVSLSFNTTTISVGASGAIFGLI
                     GSIFAMMYVSKTFNKKMLGQLLIALVILVGVSLFMSNINIVAHIGGFIGGLLITLIGY
                     YYKVNRNIFWILLIGMLVIFIALQIRIFTIKEDNIYNKLIKDDMTSGNYDNAQNIVKQ
                     TINKNYADDQTYYLSGMIMATINSKSEGMTEWERGLRMFPKSGLLNFELAIANRSLND
                     DEKALKYVRKALNADPKNTDYINLEKELTKSNESKNK"
     gene            complement(5596..6135)
                     /locus_tag="O0293_000451"
     CDS             complement(5596..6135)
                     /locus_tag="O0293_000451"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCY0279361.1"
                     /translation="MTKNEIRKYILHKMKNFNKAEKRKADTWLRNQFFATEEYKEANA
                     IALVLSFNHEVDTFSIIEQALMDHKRIFVPKMDYLNHQMTFKEIFNLKDIDVDNKGIY
                     YPTSKGETTNNLDLIVVPGVGFQDDGYRIGYGGGYYDRFLANYQTKTISLLYDFQITS
                     FEPESFDQPVDKLIIYQAA"
     gene            complement(6344..6493)
                     /gene="rpmG"
                     /locus_tag="O0293_000452"
     CDS             complement(6344..6493)
                     /gene="rpmG"
                     /locus_tag="O0293_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCY0279362.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERVEMKKFCSRENKQTL
                     HRETK"
     gene            complement(6606..8681)
                     /locus_tag="O0293_000453"
     CDS             complement(6606..8681)
                     /locus_tag="O0293_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HCY0279363.1"
                     /translation="MLKRLKEKSNDEIVQNTINKRINFIFGVIIFIFAVLVLRLGYLQ
                     IAQGSHYKQIIKNDENITVNESVPRGRILDRNGKVLVDNASKMAITYTRGRKTTQSEM
                     LDTAEKLSKLIKMDTKKITERDKKDFWIQLHPKKAKAMMTKEQAMLADGSIKQDQYDK
                     QLLSKIGKSQLDELSSKDLQVLAIFREMNAGTVLDPQMIKNEDVSEKEYAAVSQQLSK
                     LPGVNTSMDWDRKYPYGDTLRGIFGDVSTPAEGIPKELTEHYLSKGYSRNDRVGKSYL
                     EYQYEDVLRGKKKEMKYTTDKSGKVTSSEVLNPGARGQDLKLTIDIDLQKEVEALLDK
                     QIKKLRSQGAKDMDNAMMVVQNPKNGDILALAGKQINKSGKMTDYDIGTFTSQFAVGS
                     SVKGGTLLAGYQNKAIKVGETMVDEPLHFQGGLTKRSYFNKNGHVTINDKQALMHSSN
                     VYMFKTALKLAGDPYYSGMALPSDISSPAQKLRRGLNQVGLGVKTGIDLPNETRGQIE
                     PLTNNPGNYLDLSIGQYDTYTPLQLSQYVSTIANDGYRIQPHIGLTIHESTNKDEVGP
                     LKKKINGTVLNKVNNTEKEIKQIQEGFKMAFNDKDGTGYVSFKDTVVPTAGKTGTAEV
                     FQNGEPRVNSTYIGYAPIDDPKLAFSIVYTNQPVPPPWLTGGDLGRDVINYYFKQLGK
                     NDKNKDKDK"
     gene            complement(8802..9401)
                     /locus_tag="O0293_000454"
     CDS             complement(8802..9401)
                     /locus_tag="O0293_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCY0279364.1"
                     /translation="MAFELPKLPYAFDALEPHFDKETMEIHHDRHHNTYVTKLNAAVE
                     GTDLESKSIEEIVANLDSVPANIQTAVRNNGGGHLNHSLFWELLSPNSEEKGTVVEKI
                     KEQWGSLEEFKKEFADKAAARFGSGWAWLVVNNGQLEIVTTPNQDNPLTEGKTPILGL
                     DVWEHAYYLKYQNKRPDYIGAFWNVVNWEKVDELYNATK"
     gene            complement(9678..10088)
                     /locus_tag="O0293_000455"
     CDS             complement(9678..10088)
                     /locus_tag="O0293_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCY0279365.1"
                     /translation="MNTNDAIKILKENGLKYTDKRKDMLDIFVEEDKYINAKYIQQVM
                     DENYPGISFDTIYRNLHLFKDLGIIENTELDGEMKFRIACTNHHHHHFICEKCGDTKV
                     IDYCPIDQIKLSLPGVNIHKHKLEVYGVCESCQD"
     gene            complement(10075..10938)
                     /locus_tag="O0293_000456"
     CDS             complement(10075..10938)
                     /locus_tag="O0293_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCY0279366.1"
                     /translation="MIDALLNFDFMRYSLISGILIGFIAPLIGAFIVVRRLSLIADAL
                     SHVTLGGISFGMFLLTIMPTLVFINPMWFGILFAIVGALLIEKLRTSYTAYQEIAIPI
                     IMSAGIALSAIFISLADGFNQEIVGLLFGSISAVNISDLTTIIVIAIIVVLFITLFYK
                     ELFILSFDEEYSKVIGIPKWIQFLFIVIVAMVISASMRVVGILLVSALITLPIAISMR
                     ITKSFKQLILLSVFLGELSVILGLVLAFYMDISPGGVIVVLLVILLMITMAYQKMRMK
                     FKKGANINEYK"
     gene            complement(10980..11765)
                     /locus_tag="O0293_000457"
     CDS             complement(10980..11765)
                     /locus_tag="O0293_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCY0279367.1"
                     /translation="MRGENTMTPVFELKNVNYYYDHKKVLENINIKINKGEFLAIVGP
                     NGAGKSTLLKLILGLLPLQSGEIFVEGIDFKNKKTSIKLSYVSQKANAFNSGFPASVK
                     EVVLSGLTKTKRLFQTFNSKDNEKVIKVLERLNISDLIHKNIAELSGGQQQRVMIARA
                     LISEPAVLVLDEPTNGIDAKHVSEFYNTLDQLKQEGITIILVTHDIGVVADTATEVAC
                     LNKHLHFHGTTDEFKSLDEVEISKIYGHPVRFVDHQHNRECCN"
     gene            complement(11891..12781)
                     /locus_tag="O0293_000458"
     CDS             complement(11891..12781)
                     /locus_tag="O0293_000458"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCY0279368.1"
                     /translation="MLLGSHVSMSGKKMLEGSAIEAHEYGETTFMIYTGAPQNTRRKS
                     IEDLNITKGHEVMEKYGLSNIVVHAPYIINIANTTKPETFNLGVDFLQQEIERTQAIG
                     AKDIVLHPGAHVGAGVDAGINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEEL
                     ARIIDGVHNNERLSVCFDTCHTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSK
                     NDRGAQKDRHENIGFGYIGFDALNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLE
                     IEMLKQQQFDPELKNKVMQQ"
     gene            complement(12791..14137)
                     /locus_tag="O0293_000459"
     CDS             complement(12791..14137)
                     /locus_tag="O0293_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCY0279369.1"
                     /translation="MAKHPFEQFNLESSLIDAVKDLNFEKPTEIQNRIIPRILKRTNL
                     IGQSQTGTGKSHAFLLPLMQLIDSEIKEPQAIVVAPTRELAQQLYDAANHLSQFKAGV
                     SVKVFIGGTDIEKDRQRCNAQPQLIIGTPTRINDLAKTGHLHVHLASYLVIDEADLMI
                     DLGLIEDVDYIAARLEDNANIAVFSATIPQQLQPFLNKYLSHPEYVAVDSKKQNKKNI
                     EFYLIPTKGAAKVEKTLNLIDILNPYLCIIFCNSRDNANDLARSLNEAGIKVGMIHGG
                     LTPRERKQQMKRIRNLEFQYVIASDLASRGIDIEGVSHVINFDVPNDIDFFTHRVGRT
                     GRGNYKGVAITLYSPDEEHNISLIEDRGFVFNTVDIKDGELKEVKAHNQRQARMRKDD
                     HLTNQVKNKVRSKIKNKVKPGYKKKFKQEVEKMKRQERKQFSKQQNRQKRKQNKKG"
     gene            complement(14251..15351)
                     /locus_tag="O0293_000460"
     CDS             complement(14251..15351)
                     /locus_tag="O0293_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCY0279370.1"
                     /translation="MKIADLMTLLDHHVPFSTAESWDNVGLLIGDEDVEVTGVLTALD
                     CTLEVVNEAIEKGYNTIISHHPLIFKGVTSLKANGYGLIIRKLIQHDINLIAMHTNLD
                     VNPYGVNMMLAKAMGLKNISIINNQQDVYYKVQTYIPKDNVGPFKDKLSENGLAQEGN
                     YEYCFFESEGRGQFKPVGEANPTIGQIDKIEYVDEVKIEFMIDAYQKSRAEQLIKQYH
                     PYETPVFDFIEIKQTSLYGLGVMAEVDNQMTLEDFAADIKSKLNIPSVRFVGESNQKI
                     KRIAIIGGSGIGYEYQAVQQGADVFVTGDIKHHDALDAKIHGVNLIDINHYSEYVMKE
                     GLKALLMNWFNTEKINLDVEASTINTDPFQYI"
     gene            complement(15354..16031)
                     /locus_tag="O0293_000461"
     CDS             complement(15354..16031)
                     /locus_tag="O0293_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HCY0279371.1"
                     /translation="MISLNNRLTTVSRFLKQGTIADIGSDHAYLPIYAIQNHLCECGI
                     AGEVIQGPFQAAVKNVAANQLVDRIDVRLGDGLSVIQPEDVIDNITICGMGGPLIAKI
                     LKDGQDKLSQHPRLILQSNIQTENLRQTLQQLNYEIIDEIIMEEKGHIYEIVVAEYST
                     ELIELSSDELKFGPKLLNNKNEYFIKKWQRELEALYHIKSKLNTEQHHQRLAQINDEI
                     AVIERVL"
     gene            complement(16162..17268)
                     /gene="rpoD"
                     /locus_tag="O0293_000462"
     CDS             complement(16162..17268)
                     /gene="rpoD"
                     /locus_tag="O0293_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCY0279372.1"
                     /translation="MSDNTVKIKKQTIDPTLTLEDVKKQLIEKGKKEGHLSHEEIAEK
                     LQNFDIDSDQMDDFFDQLNDNDISLVNEKDSSDTDEKLNPSDLSAPPGVKINDPVRMY
                     LKEIGRVNLLSAQEEIELAKRIEQGDEVAKSRLAEANLRLVVSIAKRYVGRGMLFLDL
                     IQEGNMGLIKAVEKFDFNKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LIRVQRQLLQDLGRDPAPEEIGEEMDLPAEKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGK
                     VFGVTRERIRQIEAKALRKLRHPSRSKRLKDFMD"
     gene            complement(17492..19291)
                     /locus_tag="O0293_000463"
     CDS             complement(17492..19291)
                     /locus_tag="O0293_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCY0279373.1"
                     /translation="MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEK
                     TPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQS
                     NSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGF
                     APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVG
                     YSGRTYTGQEPKYLNSPETPIFQKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDT
                     AGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATLKTGQNLLQQGLNVF
                     VIQLPSGMDPDEYIGKYGNDAFTAFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKE
                     LSHDISLMKSSILQQKALNDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYIEPE
                     PIGMAQFDNLSRQEKAERAFLKHLMRDKDTFLNYYESVDKDNFTNQHFKYVFEVLHDF
                     YAENDQYNISDAVQYVNSNELRETLISLEQYNLNDEPYENEIDDYVNVINEKGQETIE
                     SLNHKLREATRIGDVELQKYYLQQIVAKNKERM"
     gene            complement(19352..20170)
                     /locus_tag="O0293_000464"
     CDS             complement(19352..20170)
                     /locus_tag="O0293_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HCY0279374.1"
                     /translation="MEKIKIIVASDSIGETAELVARAGISQFNPKQCKNELLRYPYIE
                     SFEDVDEVIQVAKDTNAIIVYTLIKPEMKQYMSEKVAEFQLKSVDIMGPLMDLLSASV
                     EEKPYNEPGIVHRLDDAYFKKIDAIEFAVKYDDGKDPKGLPKADIVLLGISRTSKTPL
                     SQYLAHKSYKVMNVPIVPEVTPPDGLYDINPKKCIALKISEEKLNRIRKERLKQLGLG
                     DTARYATEARIQEELNYFEEIVSEIGCPVIDVSQKAIEETANDIIHYIEQNKSK"
     gene            complement(20181..20804)
                     /locus_tag="O0293_000465"
     CDS             complement(20181..20804)
                     /locus_tag="O0293_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0279375.1"
                     /translation="MELSQRQERIIEIVKTKGPITGEQIADKLNLTRATLRPDLAILT
                     MSGFLEARPRVGYYYSGKSKGKFFNEKLRQFEVKDYMSQPVVLRENTTVYDAICTIFL
                     EDVSTLFIINEDNDFVGVCSRKDLLRASMIGADIHKVPISVNMTRMPNVTYLEESELV
                     IYAADRMIEKEIDSIPIVRKKDNQKYEVIGRISKTTIAKLLVALYKE"
     gene            21139..22530
                     /locus_tag="O0293_000466"
     CDS             21139..22530
                     /locus_tag="O0293_000466"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HCY0279376.1"
                     /translation="MAKDMDTIVSLAKHRGFVFPGSDIYGGLSNTWDYGPLGVELKNN
                     VKKAWWQKFITQSPFNVGIDAAILMNPKVWEASGHLNNFNDPMIDNKDSKIRYRADKL
                     IEDYMQDVKGDENFIADGLSFEQMKKIIDDEGIVCPVSKTANWTEIRQFNLMFKTFQG
                     VTEDSTNEIFLRPETAQGIFVNYKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFIFRT
                     REFEQMELEFFCKPGEEIEWQNYWKTFASDWLTSLNMSSENMRLRDHDEDELSHYSNA
                     TTDIEYKFPFGWGELWGIASRTDFDLRKHAEHSGEDFRYHDPETNEKYIPYCIEPSLG
                     ADRVTLAFLCDAYDEEGVEGSKDARTVLHFHPALAPYKAAILPLSKKLSGEAIKIFEQ
                     LSSKFSIDFDESQSIGKRYRRQDEIGTPYCVTFDFDSLEDNQVTVRDRDSMEQVRMPI
                     SELEAFLTEKTKF"
     gene            complement(22680..23426)
                     /gene="recO"
                     /locus_tag="O0293_000467"
     CDS             complement(22680..23426)
                     /gene="recO"
                     /locus_tag="O0293_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCY0279377.1"
                     /translation="MRQKGIIIKAVDYGESDKIITILNEHGAKVPLMARRAKKVKTGL
                     QAQTQLFVYGLFIYNQWRGMGTLNSVDVISQHYKLQMDLYVSSYAALAAETIERSMDE
                     GDIAPYNYQLLQFVLEKIESGTSAQLMSVVVMLKCMKRFGFTASFNRCAVSGNDTQAD
                     LIGYSFKFDGAISRQEASKDVHAVILSNKTLYLLDVLQKLPIDKMNSLNIHQEIIDEM
                     SDIILMLYREYAGMFFKSQKLINQLKRLEQ"
     gene            complement(23454..24353)
                     /gene="era"
                     /locus_tag="O0293_000468"
     CDS             complement(23454..24353)
                     /gene="era"
                     /locus_tag="O0293_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCY0279378.1"
                     /translation="MTEHKSGFVSIIGRPNVGKSTFVNRVIGHKIAIMSDKAQTTRNK
                     IQGVMTRDDAQIIFIDTPGIHKPKHKLGDYMMKVAKNTLSEIDAIMFMVNANEEIGRG
                     DEYIIEMLKNVKTPVFLVLNKIDLVHPDELMPKIEEYQSYMDFTEIVPISALEGLNVD
                     HFIDVLKTYLPEGPKYYPDDQISDHPEQFVVGEIIREKILHLTSEEIPHAIGVNVDRM
                     VKESEDRVHIEATIYVERDSQKGIVIGKGGKKLKEVGKRARRDIEMLLGSKVYLELWV
                     KVQRDWRNKVNFIRQIGYVEDQD"
     gene            complement(24354..24758)
                     /gene="cdd"
                     /locus_tag="O0293_000469"
     CDS             complement(24354..24758)
                     /gene="cdd"
                     /locus_tag="O0293_000469"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCY0279379.1"
                     /translation="MSYQPHYFQEVRKAQQESYSPYSQFKVGAYLKTKDGRTFYGTNV
                     ENASYPLSICAERASLVSAISQGYRPGDFESITVTVDADKPSSPCGACRQVLKELCDD
                     DMPVYMTNHKGDMVMMTVAELLPFGFSGKDLE"
     gene            complement(24769..25113)
                     /locus_tag="O0293_000470"
     CDS             complement(24769..25113)
                     /locus_tag="O0293_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCY0279380.1"
                     /translation="MKRFKYALDGLKILIQKDYKFLLHVFAMIVAIVFGLVLNINRIE
                     WIFILIAIALVLTVEALNTAIEYVVDLVTVEYHDLAKYAKDIAAFSVLIVSILAFIIG
                     LIVFLPHFIALF"
     gene            complement(25116..25583)
                     /gene="ybeY"
                     /locus_tag="O0293_000471"
     CDS             complement(25116..25583)
                     /gene="ybeY"
                     /locus_tag="O0293_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCY0279381.1"
                     /translation="MFTIDFSDHTGLVKDAWYKQIEDLLEFAKKEEHIEDDAELSVTF
                     VDKQEIQEINRTYRDKDKVTDVISFALEEDEPEIDFSGLDIPRVLGDIIICTDVAQEQ
                     ANNYGHSFERELGFLALHGFLHLLGYDHMTEADEKEMFGRQDTILNAYGLTRD"
     gene            complement(25584..26531)
                     /locus_tag="O0293_000472"
     CDS             complement(25584..26531)
                     /locus_tag="O0293_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCY0279382.1"
                     /translation="MPGIIQIDDMNQSQALIGNNDEHLKAIEESFDVVIHARGQEVAV
                     KGTKIENVEKAESVLINLLKVIDLGNNITIKDVEAAIKMAHNNTIQHLLDLYDEEITK
                     DAFGKTIRAKTMGQRIYVNAMKNNDLVFGIGPAGTGKTFLAVVYAAKQLRKGAVKRIV
                     LTRPAVEAGESLGFLPGDLKEKVDPYLRPLYDGLYTVLGREQTERFIERGIIEIAPLA
                     YMRGRTLEDAFVILDEAQNTTHAQMKMFLTRLGFGSKMVVTGDQTQIDLPKGVKSGLK
                     EAVSRLHNVKGISILKLDQSDVVRHPLVSKIIEHYEGEN"
     gene            complement(26835..27533)
                     /locus_tag="O0293_000473"
     CDS             complement(26835..27533)
                     /locus_tag="O0293_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HCY0279383.1"
                     /translation="MSVGILIFVISVIISIITTMRENSHKDRQNQKPPQKTSTDNEPK
                     KGGFFEEIERTFKEISEELNEEEKKSSKRKYDDTLPPLFDELPKEEPKSKPVVEPMAP
                     KKQQETKPMTEKPITVPKAEPVEQKHRPSRQDNSDEIRRQLEKSLRDDIKTIRTDIDR
                     EKEKQIAKMEKRARDIIEDKYLSERTKRLKLKQLLNSQNVEKDLTKSAFQFDKDEVIN
                     GMIWSEILAKPKQL"
     gene            complement(27550..28539)
                     /gene="floA"
                     /locus_tag="O0293_000474"
     CDS             complement(27550..28539)
                     /gene="floA"
                     /locus_tag="O0293_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500186.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HCY0279384.1"
                     /translation="MFSLSFIVIAVIIVVALLILFSFVPIGLWISALAAGVHVGIGTL
                     VGMRLRRVSPRKVIAPLIKAHKAGLALTTNQLESHYLAGGNVDRVVDANIAAQRADID
                     LPFERAAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMNGIEVKAKARITVRANIAR
                     LVGGAGEETIIARVGEGIVSTIGSSKHHTEVLENPDNISKTVLSKGLDSGTAFEILSI
                     DIADVDISKNIGADLQTEQALADKNIAQAKAEERRAMAVATEQEMKARVQEMHAKVVE
                     AESEVPLAMAEALRSGNISVKDYYNLKNIEADTGMRNAINKRTDQSDDESPEH"
     gene            complement(28557..29240)
                     /locus_tag="O0293_000475"
     CDS             complement(28557..29240)
                     /locus_tag="O0293_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCY0279385.1"
                     /translation="MTTILESTAGDTWVEQVSNIIVQPIFTLILTCLTFLGFVYQLYS
                     KKINAAGIIATLSLLILFLGFLIQGNVNMHSILIFSIGVILVVIELFVVGAVIGIIGM
                     ILITISITTLGDNLLFMLANVIVALILTIVEWVILVKIFNRKIPFLDKVILKDSTNSE
                     SGYNSHDNRSHLVGKTAQTVTDLRPAGIIFCENERIDAVSDGNFILRNKTVKILEVEG
                     TRVVVREVD"
BASE COUNT         9240 a         5397 c         3946 g        10803 t
ORIGIN      
        1 aataaaccta aaatcaaaaa taaaataggc tgtaaaaact gagtctgttt tatcgcttta
       61 tcttctattt gttttactaa tatttgcgaa tataacttta gttctacttc tagcttccct
      121 ctcttttcac cttgtagcac aaacttaatt aattgaggct taaagcattt tagtttttct
      181 aaaatttgag gcaaaccata tcccatttct gaataagtta ataagtattt acctagaaac
      241 tgtctaaatg gatcactact atggttaata taaacgtcta ctattgattg aagtgtaata
      301 ccatttttat aaaacaacac taattcatta gttacaaaat aagttttaaa taattggaaa
      361 tagcctgata ttagcggtag tttcatcaca aagtttatct tattgagcat atttaaattg
      421 ttataaatta atttcataat aatagccaac atagatacta ttatgagcat tactacaatt
      481 atagtaggta agctggtaat gaaaaaagac aatgtttttt gaaaagaaga tagttgaata
      541 ttcatagaag tatataattg ttgaaactgt ggaattactg tgaggtttaa tataataatc
      601 atagcaataa agatagaaac tagtattaag gggtattgca gtgtctttaa caatcgttgt
      661 tctgactttc tattcacttt catataattt acggtttctt ctagaacgtc tataatattg
      721 ccaaatcttt ctgccaaata tacttgcatg acgatagtat cgctataacc tatcagtgat
      781 aatatttgat tgcatggtgc accatttgaa atttcactta gaatggtggt acctaattgc
      841 ttatttttat atgtcatttg aagatttaaa aattgaaaac tttgatacag agtgaaacca
      901 tatttcaaca aattacataa atttgaaagt aagtcgattt gttgggcctt acttaattgt
      961 cgcttcttag aatgtagttt aaatgtattt atgcattgta gtttcacaaa tgacacctgc
     1021 ttttgtcata tcatcaagtt tatcttctaa gttcttaaat gatgatggta atgaatgatt
     1081 atgggaaaag aaatatcgga gttgttgctg agatagaatt tcacatacta attgtcgctg
     1141 ttgcttaata gtagttacaa gtcgttggtt tataattaag ttagttgcct gtatcaattc
     1201 ttgtacagaa atgcccattt ctaatagcct taaaatagca cctttacaat cagttgcatg
     1261 caatgtagtc agaacaaggt gaccacttaa actagcctgt ataacacact tggcaacatc
     1321 tttatctctg atttcaccta ttaaaataac atcaggatca catcttaaaa tagctttaaa
     1381 cgaatttaca tagtttatgc cagctttatc attcacatta atttggacga taccaggaat
     1441 ttgcatctct acaggatcct ctatagaaat tacatttaaa ttcaaggctt tattcgcgta
     1501 tgagaccatt tgatacatta atgtactctt tcctgaacca gttggtccac taaacaatag
     1561 taatccttgt ttcttattca tgaggtgttt aaaatcattg aatttataag ttgatttctg
     1621 tggttgaaaa aattgaggta caattctgat aacacaactt tcttggccaa gtgacaatgg
     1681 taaagttgat attctcaaaa aatatatttt attgaaaagg taactatatc gaccgctctg
     1741 tgcgacttgc tgtgtagaaa catcaagccc agcttgaaac ttcatataaa ctaataactt
     1801 ttgataaatg ctattcccaa tttgttcata ctgctccaag ttatcattaa ttctaaattt
     1861 aatacttact tcatttttaa ctggaataaa atgtacatca ctcgctttca tttctatcgc
     1921 tttattaatt atttcttgaa atagaatctt caaaaaaaca cctcctacat ataatcacgt
     1981 aggaggtgtt tttattactt caatttaacc gtgtaaaaat ggatttaatt gttcatcatc
     2041 aaccgtcgta tatggaccat gtccagggaa taaaggtaaa tcgccttcta attcaaatat
     2101 tttatcttga atagaatcaa ctagcgtttc ataatcacct ttatataaat ctgtacgtcc
     2161 gattccatta ttaaataatg tatctccaac aactgcgaat tcatcgaaca catatgttaa
     2221 acttcctggt gaatgtccag gtgtgtgtaa cacattaaac ttaaatcctt ctatttctgt
     2281 gctaccttcg tttaactttt caggagttac cttacttgta ataattggta atccatattg
     2341 cttaaattta tctgccccat ttttaacggg atcttttaga aaatcaaact ctgcttcatg
     2401 catataaacc gggacatcga atcgatcaac tatatcatcg actgctccga tatgatcaaa
     2461 gtgtgcatgt gttaataaaa tagcttttaa cggtttattt atttggttta attttttaat
     2521 aattttttca ctttcacctg aagggtcaat cagaataaca gctttgtcat tttcgatgaa
     2581 atacgtatta gtatcaacta agcctaaagt taagcttgaa atcctcatag gttttcacca
     2641 ctattttcta aatgtttttg tactgatgtt agtttatcat tcatttttct agatttgtct
     2701 cgtcggtcat caatacggat atttgtacaa actctactta aacctttatc aaaaggtaat
     2761 tcatgtatca cttgcacaac ttctaataca tcgcttaatt caccttcaat tagagtattc
     2821 attggtgtta attgaaaatc aattttaccc attgctttat attcttgaag ttttttctga
     2881 atatctgcaa tatatttact aacactcgga ccttccgttc caactggaat aacaaccaca
     2941 tcaacaatag ccattatttt accccctctt tatctaatac ataggtcttg attaatcctg
     3001 ctgctcctgt aatacctgca tcattaccta atttcgcttg tacaatttca gtttcaaatt
     3061 gagcaggtgc aaatgttaaa ttatgatatt ctgttttaat attttcaatt aaaataggtc
     3121 ctgcagtaga cattcctcca cctagaacga tatatttcgg attacttgta acactaataa
     3181 tactacataa atatccaata tagtttgcaa ccttttcagt aatgaaaata cagaattggt
     3241 caccagcttt tgccgcatca aaaacagctt ttgctgtaac cttattttct ttaatcaatt
     3301 ctaatataga agatctaaac gtcaacttcg gatagtagaa gttaactaag ttaacaacgc
     3361 ctgtcgctga agcaactgtt tcaatacatc cagaacgacc acaattacat ttaaatcgtt
     3421 gatcgaagtc tgctctaaaa tgacctattt ctgcgccaga gccattatga ccatgtacga
     3481 tttcaccatt tgaaataatt cctccaccta gacctgtacc aagtgtgatg gcaacaacat
     3541 catcggcacc ttcaccagca cctttgtgtt tctcccctaa agcagctatg ttagcatcat
     3601 tatctacata cactggacaa tcaacgaatt gttcaaaaat ctcacgtaca ttaacttttt
     3661 ctggccaata taagtttact gctccattta ctgtaccttt ttcaaagtca acaggacctg
     3721 gtacaccaat acctacgcca agtacatttg aaaaattata attattttca tttacttttt
     3781 caacaaacga atcataaatt cctttcaaaa gtgtatatcc tgtactatca gatgtatcag
     3841 tgtgaataga ccatttatgt aattgttcta attcaggtgt gaaaatacct aatttacaag
     3901 tcgtcccgcc tacatcagct gctaaaataa tttttgtcat ttctgttcat tccttctctg
     3961 attaattatc aacgtacatt tcaaatattc ttccttagat aacagttcat attgatacaa
     4021 tgatgaaatc tcctgttgaa tcatttcgta catatcttct ggatttttaa aatatattag
     4081 aaatccgtaa cttttcaata actgttgtac atcataaaag ttatttattt ttcgactcat
     4141 ttgatttagt caactctttt tctaagttaa tataatctgt atttttaggg tctgcattta
     4201 atgctttacg cacatatttt aatgcttttt catcatcatt taatgaacga tttgctatcg
     4261 ctaactcaaa atttaataaa cctgatttag gaaacattct aagtcctcgc tcccattctg
     4321 tcataccttc ggacttagaa ttgatagtag ccataatcat accacttaaa taatatgttt
     4381 gatcatcggc ataattttta tttattgtct gcttcacaat attttgagca ttatcataat
     4441 taccactagt catatcatct ttgatcaatt tattataaat attatcttct ttaattgtaa
     4501 aaattctaat ttgaagtgca ataaatataa caagcatacc aattagtaaa atccaaaaaa
     4561 tattacgatt cactttatag taatagccaa ttaaagttat taataaacca ccaatgaatc
     4621 ctccaatatg cgccacaata tttatatttg acataaacag agaaacacca actaatatca
     4681 ctaatgcaat taataactgt cctaacattt ttttgttaaa tgtttttgaa acatacatca
     4741 tcgcaaaaat tgatccaatc agaccaaata tagcaccact agccccaact gaaattgtag
     4801 tcgtattaaa tgatagtgat acaaagtttc caaacaaccc tgcaataaag tatacagtta
     4861 acatccgcca tgaaccaata attgcttcga ctattttacc aaaaataaat aatgaaagca
     4921 tattcataag tatatgttca aaactaaaat gtaaaaacat cgatgtaaca attcgatacc
     4981 attcaccatg tacgacatta aaatgcacta acccgccaac atctaataat tttacatccg
     5041 aaaaattatt taaatataaa atcatacata accatattaa gacatttaca aatattattg
     5101 tgtatgttgc cggtgaaaat ttctgcatat atttatctaa aaagttatcg gttaatactt
     5161 tgcgtttata gaacatatat gtctttttat cattatcttc tgaaataagt ctagccaaaa
     5221 atatattcgg catatgcttt atcaaatctt ttgtgtgtct tataacattg aatttaattc
     5281 taattggcga aacttcattt aattgttctt ctgaaaattc cgattctgta aaataataaa
     5341 attcaaaact ttgtggttcg aaagaaataa aatttgctat ttcatctttg tgttcaagca
     5401 ctttcgcttt gtcaaaacgt atttcttggg tagacttgat atgttgttta aaaatgacta
     5461 cttgtttttt tcttttatgg gctaaccaaa tttcttggtc atctttttcc ctactgacaa
     5521 tatcaaaatt caagtaccta atccaataat atattgtttt ccaaaattgt ttgtctatgt
     5581 tcatttagtc ctccactatg ctgcttgata tataatcaat ttatcgactg gttgatcgaa
     5641 tgattccggt tcaaatgatg ttatttgaaa atcgtataat aagcttattg tctttgtctg
     5701 ataattagct aaaaacctgt cgtaatagcc accaccatac ccaattctat atccatcgtc
     5761 ttgaaatcca acaccaggaa caacaattaa atctaggtta ttcgttgttt caccttttga
     5821 agttggatag taaatcccct tattatcgac atcaatatct ttgagattaa atatctcttt
     5881 aaaagtcatt tgatgattta aataatccat tttcggtaca aaaatacgtt tatgatccat
     5941 taaggcttgt tcaataatag aaaaagtatc tacttcatga ttaaaagaaa gaactagcgc
     6001 aattgcgttt gcttctttgt attcttcagt tgcaaaaaat tgatttctta accatgtgtc
     6061 tgcttttcgc ttttcagctt tattaaaatt cttcatttta tgtaaaatgt attttctaat
     6121 ctcattttta gtcacattat cacccacttt attcaattct attatatcgt taataacaat
     6181 acatgtcatt atgttgtcat gatgaattat gcgacaattc taaaatttat tatttcaatt
     6241 gaaaatcttt tatcatttca tacgtgtgca taattaaaaa ggtgaaacct cttattttga
     6301 gatttcacct tttttattgt taatttaaat ttaattttaa tgattatttt gtttcacggt
     6361 gtaaagtttg tttgttttca cgtgaacaga atttcttcat ttcaacacgt tctggattat
     6421 ttcttttgtt cttagttgta atgtagtttc tgtcaccaca ttccgtacaa gctaaagtta
     6481 cgtttacgcg catggttatt cctccttatc atttctaaat acgactttat tattatacca
     6541 acatctataa tatttgcaaa gttaaaattt tacaaccatg cgctacacaa tcgtcaggtt
     6601 aaattttatt tgtctttgtc tttattttta tcatttttac ctaactgctt aaagtagtag
     6661 ttaattacat ctctacctaa gtctccacct gttaaccatg gtggtggtac aggctgattt
     6721 gtatatacaa ttgaaaacgc taattttgga tcatcaattg gcgcgtatcc tatataagta
     6781 gagttaactc ttggctctcc gttttggaac acttctgcgg tacccgtttt accagcagta
     6841 ggtactactg tatctttaaa actaacatat ccagtaccat ctttatcatt aaatgccatt
     6901 ttgaatcctt cttgaatttg tttgatttcc ttttcagtat tattaacctt gttcaagaca
     6961 gtgccattaa ttttcttctt gagtggacca acctcatctt tattagttga ttcatgaatc
     7021 gttaatccaa tgtgtggctg tattctataa ccatcattcg ctatagttga aacatattgt
     7081 gataattgta atggtgtata ggtatcatat tgaccaattg ataaatctag ataattacct
     7141 ggattatttg ttaatggttc gatttgacct cttgtttcat ttggtaaatc tatccctgtt
     7201 ttcacaccta agcctacttg atttaatcct cttcttagct tttgggcagg tgaacttatg
     7261 tctgaaggta aagccatacc agaataataa gggtctcccg ctaattttaa tgctgtttta
     7321 aacatatata cgtttgatga atgcatcaaa gcttgcttat cattaatagt tacatgcccg
     7381 tttttattga agtatgatcg ttttgtcaaa ccaccttgga aatgtaatgg ttcatcgacc
     7441 attgtttctc caactttgat agctttattc tgataaccgg ctaataatgt tccacctttt
     7501 acagaagatc caaccgcaaa ttgagaagta aacgtaccaa tgtcataatc agtcatttta
     7561 ccactcttat taatctgctt tccggcaagc gcaagaatgt ctccattttt aggattttgt
     7621 acaaccatca ttgcattatc catatctttg gcaccttgac tgcgaagctt cttaatttgt
     7681 ttatctaata atgcttctac ttctttttga agatctatat cgatcgttaa tttcaaatct
     7741 tgaccgcgag cgccaggatt taacacttct gaagatgtaa ctttaccaga tttgtccgtt
     7801 gtgtatttca tttctttctt cttaccacgc aatacatctt catattgata ttctaggtaa
     7861 gattttccaa cacgatcatt gcgtgaatat cctttggata agtaatgttc tgtcaattct
     7921 tttggaatac cttcagcagg tgtcgataca tctccgaata tacctcttaa agtatcgcca
     7981 tatggatatt ttctatccca atccatagac gtgttaacac ctggtaattt ggaaagttgc
     8041 tgagaaactg ctgcatactc tttttcactg acatcttcat tttttatcat ttgtggatct
     8101 aaaactgttc ctgcattcat ctctcgaaaa atagctaaaa cttgtaaatc tttagaagac
     8161 aattcatcta attgtgattt tccgattttc gataacagtt gtttatcata ttgatcttgt
     8221 ttaatacttc catctgctaa catagcttgt tcttttgtca tcattgcttt tgctttttta
     8281 ggatgcaact gaatccagaa atctttctta tcacgttctg taattttctt agtatccatc
     8341 ttgattagct ttgataactt ttcagccgta tccaacattt ccgattgtgt tgtttttcga
     8401 cccctagtat atgtaatagc cattttagaa gcattatcaa ctaaaacttt cccatttctg
     8461 tctaaaatac gacctcttgg cacagactca ttcactgtaa tgttttcatc attttttata
     8521 atttgtttat aatgtgagcc ttgtgcgatt tgtaaataac ctaaacgtag tactagtact
     8581 gcaaaaataa atataatcac accaaatata aagttaattc tcttgttaat tgtattttga
     8641 acgatttcat catttgattt ttcttttagt ctttttaaca aaactaccta cctctattca
     8701 aagtctttca ctttaaatca tatatgaatt tagaaattat ttctatcttt ttgacaaaaa
     8761 aataacggtc tcatttaaga gaccgaacaa gtaatcatac tttattttgt tgcattatat
     8821 aattcgtcaa ctttttccca gttaactaca ttccaaaatg cgccaatgta gtcagggcgt
     8881 ttgttttgat attttaggta ataagcgtgt tcccatacgt ctaaacctaa aataggtgtt
     8941 ttaccctcag ttaatggatt atcttggttt ggtgtagtca caatttctaa ctggccattg
     9001 tttacgacta accaagccca acctgaacca aagcgtgcag ctgctttgtc agcaaattct
     9061 tttttaaatt cttctaaaga accccattgt tctttaattt tttctactac agtacctttt
     9121 tcttctgagt ttggtgaaag taactcccag aataatgaat ggtttaaatg tccaccgcca
     9181 ttattacgta cagcagtttg gatgttagct ggtacactgt ctaaattagc aacaatttct
     9241 tcaatagatt tagattctaa atctgtacct tctactgcag catttaattt cgtaacatac
     9301 gtgttatgat gtctgtcatg gtgaatttcc atagtttctt tgtcaaaatg tggttctaat
     9361 gcatcaaatg cgtatggtaa ttttggtaat tcaaaagcca taaataatca tcctcctaaa
     9421 atgtctgtaa gtaaataata acaagcagtg actggttcaa caaagaattt gcttaaattc
     9481 tactacttat tattttctct actcatttaa tataactcaa atcaaaaata attaaacatt
     9541 ttgtatataa aaagttaaca gatttgccat aaaatcatac gaacggagta tgaaatgaac
     9601 ctttatcttc tataatataa agaaatgaga tttatgcata catctgacca aatgtgcata
     9661 aatctcattt ctttatatta atcttggcaa gactcacata caccataaac ttcaagtttg
     9721 tgtttgtgaa tattaacacc aggtagtgat aattttatct gatctattgg acaataatct
     9781 attacctttg tatctccaca cttttcacag ataaaatgat gatgatgatg gtttgtacaa
     9841 gcgattctaa acttcatttc accatcaagt tctgtatttt caataattcc taaatcttta
     9901 aataagtgca ggtttctata tattgtgtcg aatgaaattc caggataatt ttcatccata
     9961 acttgttgta tatactttgc gtttatatac ttatcttctt cgacaaaaat atctaacata
    10021 tctttacgtt tatctgtata ttttaaaccg ttctctttta aaattttaat agcatcattt
    10081 gtattcattg atattagctc cctttttaaa cttcattcgc attttctgat aagccattgt
    10141 aatcataagt aaaataacaa gtagaactac aattacacca cccggagaaa tgtccatata
    10201 gaaagctagg actaagccta atattactga taattcacct aaaaatacac ttagtaatat
    10261 caattgctta aaactttttg ttattcgcat acttattgca attggtaacg tgattaacgc
    10321 acttactaac agtatcccta caacacgcat tgaggcagaa ataaccatcg ctacaataac
    10381 aataaataaa aattgaatcc atttaggaat gccaatgact ttactatatt cctcatcaaa
    10441 tgacaatata aataattctt tataaaacaa tgtaataaac agaacaacta tgatggcaat
    10501 gacaataatc gttgttaaat cacttatatt cactgcgctt attgagccaa atagcaatcc
    10561 aacaatttct tgattgaacc catcagctaa tgaaatgaag attgcactca aggcgatacc
    10621 agcactcatt ataattggaa tagcaatttc ttggtaagca gtgtatgacg ttcttaattt
    10681 ttcaattaga agcgcaccta ctattgcgaa taagattcca aaccacattg gattaataaa
    10741 tactagtgtt ggcataatag taagtaaaaa cataccgaaa gatataccac ctaaagttac
    10801 atgacttaga gcatcagcta taagtgatag tcgtctaaca acgataaaag caccgattag
    10861 aggcgcaata aaacctatca agataccact aattaaagag tacctcataa aatcaaaatt
    10921 caataatgca tctatcaatt gtgacacgcc tttccatttt aaataaactc aaatctttat
    10981 taattacaac attctcgatt atgctgatga tcgacaaaac gtacaggatg tccataaatt
    11041 tttgaaattt caacttcatc aagtgattta aactcatcag ttgtaccatg gaaatgcaaa
    11101 tgcttattta aacatgctac ttcagtagca gtatctgcta caacaccgat atcatgagta
    11161 actaagataa tggtgatacc ttcttgtttt aattgatcta aagtattata aaattcactt
    11221 acatgttttg catcaatacc attcgttggt tcatcaagta ctaatactgc aggttctgaa
    11281 atcaatgctc gagcaatcat tacacgttgt tgttgaccac ctgataattc tgctatattt
    11341 ttatgaatta aatcacttat attcagtctt tctagtactt taatcacttt ttcattatct
    11401 ttgctattaa atgtttggaa aagacgtttt gtctttgtta atccgcttaa aacaacttct
    11461 ttaacacttg ctgggaaacc tgaattaaag gcatttgctt tttgtgatac atagcttaat
    11521 ttaattgatg ttttcttatt tttaaaatca ataccttcaa caaaaatctc accactttgt
    11581 aaaggtaata accctagaat caacttcaat aatgttgatt taccagcacc atttggtcca
    11641 acaattgcta aaaattcacc tttatttatt ttaatgttta tattttctaa cactttttta
    11701 tgatcatagt agtaattgac atttttcaat tcaaagactg gtgtcatcgt attctcacct
    11761 cgcattcaac tatacaactc ctagtaacat atgtaaacag taatgtttac gactcaaaat
    11821 tagacaaaat aaagagatat gcccccttca agttttattt atcgcatttc ttgaagagag
    11881 cattatcatt ttattgttgc ataaccttat tttttaattc tgggtcaaat tgctgttgtt
    11941 ttaacatttc aatttcaagt ttatatggcg gttttttatt tttcttatct tcaccaacat
    12001 aaggtgtttc taagattttc ggaatatctt taaaactatc atgatgcaca atgtaattta
    12061 atgcatcaaa accaatgtaa ccgaagccaa tattttcatg tcggtctttt tgagcgccac
    12121 ggtcattttt agaatcattg acatgaacaa ctttgattct gtcgactcca atgattttat
    12181 caaattcatt taatacgcca tcaaagtcct ctttaacatt atatccagca tcatgcgtat
    12241 gacatgtatc aaaacatact gataaacgtt cgttattatg aactccatca ataatacgtg
    12301 ctaactcttc aaatgagcga ccaatctctg tacctttacc tgccatcgtt tcaagcgcaa
    12361 tacgtacatt attgtcattc gttaaaactt catttaatcc ttcaataatc ttattaattc
    12421 cggcatcaac accagctcca acatgcgcac ctggatgtaa tacaatatct ttagccccta
    12481 tagcttgcgt tctttcaatt tcttgttgca agaaatctac accaagatta aacgtttctg
    12541 gtttggttgt atttgcaata ttaatgatgt atggtgcatg aacaacaata ttagataaac
    12601 catatttttc catcacttca tgacctttag ttatatttaa atcttcaata cttttacggc
    12661 gcgtgttttg aggtgcacca gtataaatca taaatgttgt ttcaccatat tcatgcgctt
    12721 ctatagcaga accttctaac atctttttac cactcattga aacatgtgat cctaataaca
    12781 taaaacacac ctaacctttt ttgttttgct tacgtttttg tctattttgc tgcttactaa
    12841 attgcttacg ctcttgacgt ttcatttttt caacttcttg tttaaatttc ttcttataac
    12901 ctggtttaac tttgttttta attttacttc gaactttgtt cttcacttga ttagttaaat
    12961 ggtcatcttt gcgcattctt gcttgacgct gattgtgcgc tttaacttct tttaactcac
    13021 catctttaat atcaacagta ttgaatacaa aaccgcgatc ttctattaat gaaatattgt
    13081 gttcttcatc aggactataa agcgtaattg ctacaccttt ataattccca cgaccagttc
    13141 gtccgactct atgcgtaaag aagtcaatat catttggcac atcaaaattg atgacatgac
    13201 taacaccttc aatatcaata ccacgagatg ctaaatcgct ggcaataacg tattggaatt
    13261 ctaaattacg tatacgtttc atttgttgtt tacgttcacg tggcgttaag ccaccatgaa
    13321 tcataccaac tttaatacca gcttcattta gtgaacgtgc taaatcattt gcattatctc
    13381 tactattaca gaaaataata cataagtatg gatttagtat atcaattaaa tttaaagtct
    13441 tttcaacttt agctgcacct ttagtaggta ttaaatagaa ttcgatgttc tttttatttt
    13501 gttttttact gtcgacagct acatattctg gatgacttaa atatttattt aaaaatggtt
    13561 gtaactgttg tgggattgta gcactaaaca ccgcaatatt tgcattatct tccaatcttg
    13621 cagcaatgta atctacatct tcaattaatc ctaagtcaat cataagatcc gcttcatcaa
    13681 taactaaata tgatgctaag tgcacatgta aatgtcccgt tttagctaag tcattaattc
    13741 tagtaggggt gcctataatc aattgtggtt gtgcattaca acgttgtcta tctttctcta
    13801 tatctgtacc accaataaaa actttaactg aaacaccagc tttaaattgg cttaaatggt
    13861 tcgctgcatc gtatagttgt tgtgcaagtt ctcttgttgg tgcaactacg attgcttgtg
    13921 gttcttttat ttcactatca attaactgca ttaatggtaa taaaaatgca tgagatttcc
    13981 ctgtacccgt ttgagattga ccaattaaat ttgttctctt tagtattctt ggaataattc
    14041 gattctgaat ttcagttggt ttttcaaaat taaggtcttt cacagcgtca attaaactag
    14101 attctagatt aaattgttcg aatggatgtt ttgccatgat ttggcctcct tatatgttca
    14161 tctttactat tataaagtat aaatgtgcta ttttaaataa aaatattaaa acctcgaaat
    14221 ttcaaaattt agaaatcccg agattttagt ttaaatatat tgaaatggat ctgtattaat
    14281 tgtagatgct tcaacatcta aatttatttt ttctgtattg aaccaattca ttagtaacgc
    14341 ttttaaacct tctttcatca catattcgct gtaatgatta atatcaatta aattcacacc
    14401 atgaatttta gcatctaagg catcatgatg tttaatatca cctgtaacaa agacatctgc
    14461 gccttgttgg acagcttgat attcatatcc aatacctgaa ccaccaataa ttgcaatacg
    14521 tttaattttc tgattagact caccaacaaa acggacactt gggatattta atttagattt
    14581 aatatcagct gcgaaatctt ccaatgtcat ttgattatcc acttctgcca taacgccaag
    14641 tccataaagg gatgtttgtt ttatctcaat aaaatcaaat accggtgttt catatggatg
    14701 gtattgttta attaattgct cagcccttga cttttgatat gcatctatca taaattcaat
    14761 tttaacttca tctacatatt caattttatc aatttgtcct attgttggat tagcttcacc
    14821 aactggtttg aattgccctc ttccttcact ttcaaagaaa caatattcat aattaccttc
    14881 ttgcgctaat ccattttcac taagcttatc tttaaatggt ccaacattat ccttaggtat
    14941 atatgtttga actttatagt atacatcttg ttgattattt attattgaaa tgttcttcaa
    15001 acccatcgcc ttcgccaaca tcatattgac accatacgga tttacatcta aatttgtatg
    15061 catcgctatt aaattaatgt catgttgaat tagttttcta atgatcaaac cataaccatt
    15121 agcttttaat gatgttacgc ctttaaagat tagaggatga tgactaataa tagtattata
    15181 acctttttcg attgcttcat ttactacttc caacgtacag tctaatgctg ttaaaacacc
    15241 agtaacttca acatcttcat cacctattaa caatcctaca ttatcccaag attcagcagt
    15301 actaaatggc acatgatgat ctaacaatgt cattaaatca gctattttca ttactataac
    15361 accctttcaa ttacagcaat ttcgtcatta atttgagcta aacgttgatg atgttgttca
    15421 gtattgagtt tcgatttaat atgataaagt gcttctaact ctctttgcca tttttttata
    15481 aaatattcat ttttgttgtt cagcaatttt ggtccgaatt tcaattcatc agatgatagc
    15541 tctattaatt ctgtagaata ttctgctaca acaatttcat aaatatggcc tttttcttcc
    15601 attattattt catcaattat ttcataattc aattgttgta atgtttgtct taaattttca
    15661 gtttggatat tactttgtaa aatcaacctt ggatgttgac ttaacttatc ttgcccatct
    15721 tttaaaattt tagcaataag tggtccgccc ataccacaaa ttgtgatatt atcgattacg
    15781 tcctcaggtt gaataacact taagccatcc cctaaacgta catcaattct atctactaat
    15841 tggtttgcag ctacattttt cacagcagct tgaaaagggc cttgaataac ttctccagca
    15901 ataccgcatt cgcataaatg gttttgaatt gcatagattg gcaaataagc atgatctgag
    15961 ccaatatccg cgattgtacc ttgttttaaa aatcgactta ccgtcgttaa tcggttattt
    16021 aacgaaatca ttttattctc ctttatgaaa aaagaggttg aacattattc aatgtaccaa
    16081 cctcactcaa gttaaatctt aatctaaaaa taaataagcg cttattcatg tcttggtatc
    16141 aatcattaaa attgatataa attaatccat aaagtctttc aaacgtttac tacgacttgg
    16201 atgtcttaat tttctaagtg cttttgcttc aatttgtcga atacgttcac gtgtaacacc
    16261 gaaaacttta ccaacttctt caagtgttct tgttctgccg tcatcaagac caaatcttaa
    16321 tcgtaataca ttttcttctc tatcagttaa tgtatcaagc acatcttcta attgctcttt
    16381 taataattca taagcagcat gatctgaagg actttgtgct tcctgatcct caataaagtc
    16441 tcctaaatga ctatcatctt cttcaccaat tggtgtttct aatgaaacag gttcttgcgc
    16501 aatttttaaa atttcacgaa ctttttctgc tggtaaatcc atttcttcac caatttcttc
    16561 tggtgctgga tctcgaccta agtcctgtaa taattgacgt tgaacacgaa ttaatttatt
    16621 aattgtttct accatatgca cagggatacg aatcgtacgt gcttggtcag caattgcacg
    16681 agtgattgct tgtctaatcc accatgttgc atatgttgaa aacttaaatc ctttgttaaa
    16741 gtcaaatttt tcaacagctt taataagacc catattacct tcttggatta aatcaaggaa
    16801 taacatacca cgacctacgt atcttttagc aatacttaca actaaacgta agttcgcttc
    16861 tgcaagtctt gattttgcta cttcatcacc ttgttcaata cgtttggcta attcgatttc
    16921 ttcttgtgca cttaataagt taacacgccc aatttcttta aggtacatac gaactgggtc
    16981 atttatttta acacctggag gggcactaag atcacttgga ttcagtttct cgtcagtatc
    17041 tgaactatct ttttcattaa ctagtgaaat atcattatca tttaattgat caaagaaatc
    17101 atccatttga tcagagtcga tatcaaaatt ctgaagtttt tcagcaattt cttcatgact
    17161 taaatgaccc tcttttttac ctttttcaat taattgcttc ttaacatctt ctaatgttaa
    17221 tgtcggatca attgtttgtt ttttaatttt aactgtgtta tcagacatga aacggcctcc
    17281 cgattttaaa tatgaacatt cgaaatttat tcaatattgc tattttaaac taaattctta
    17341 attaattcat ccatattttt aattttattt tacaaattgg aactaatccc aatatttatt
    17401 tttcaatagt gttgtttaaa tgcggtaact atcatttacc cttatacatt ttgacataat
    17461 cattattcgt attattcttt aaaatcacat gctacatgcg ttctttattc ttagcaacaa
    17521 tttgctgtaa atagtatttt tgtaattcta catcgccaat ccttgtagct tcccttaatt
    17581 tatgattcaa tgactcaatt gtttcttgtc ctttttcatt aataacattg acataatcat
    17641 caatttcatt ttcatatggt tcgtcattca aattatattg ttctaagcta attagtgttt
    17701 ctctcaactc atttgaatta acatactgca cagcatcact gatattatat tgatcatttt
    17761 ccgcataaaa atcatgtaag acttcgaata catatttaaa atgctgattt gtgaagttat
    17821 ccttatcaac actttcataa taatttaaaa atgtatcttt atctctcatt aaatgtttta
    17881 aaaatgctcg ctccgctttt tcttgacggc tcaaattgtc aaattgtgcc ataccaattg
    17941 gctcaggttc aatgtaaccg ccatactcat cttctggata ataattggct ggtgcttgat
    18001 tgaattgtat ttcgttagct aattgctcag gactaacatt gaaaaatggt gcaacatcat
    18061 ttaaagcctt ttgttgcaaa atcgatgatt tcataagcga aatatcatga cttagttctt
    18121 tcaaataacg ttcatatgaa aggtcattat gtgcaatttc atcttttaat atactcactt
    18181 tataatgtgc aaatgacttt ttgtcatttt ttacaaaagc agtaaatgca tcgttgccat
    18241 acttaccaat gtattcatcc ggatccatgc ctgatggcaa ttgtataaca aatacattta
    18301 gcccttgctg taacaaattt tgacctgttt taagtgttgc ttcactaccc gcaaaatccc
    18361 catcaaacat taatgttata tttgatgtta actttcgtat aaaagtaata tgttcatctg
    18421 acaactgtgt acccattgtt gcaacaacgt ttttcaagcc agcagtatca gattttataa
    18481 catccataaa accttctagt aatacgattt catctaattt tctaattgat ttacgcgctt
    18541 tatctaagtt gtataacaac tttctttttt gaaagatagg tgtttcagga ctatttaagt
    18601 attttggttc ttgaccggta tatgttcgac ctgaatatcc aacaattctt ccttgcgcat
    18661 ttttcaaagg aaacataata cgatttcgaa atctatcgta ataactgaaa ttttcttcgt
    18721 tacgtgataa taatccggct tcatatgcta attcaatatc gtaacccttt ttttgaagaa
    18781 aatcatgaca aaaatgtgag ctatcgggtg caaagccaat gcctcgctct ttaataagcg
    18841 catctgtaaa accacgttct tgtaagtacg ttaatgcttg ttcgccttcg actgtctttg
    18901 ttaaagcgta ataataaaat tcttgtatta actcatgcat ttcaatcatt tgtaaatcat
    18961 cagaagcaat ttgaacattt gagttagatt gtgttgcctc aatatctaca gcaacattaa
    19021 ctctatcacc taattcttta accgcttcaa caaatgatat gtctttaatt tcttgagtaa
    19081 attgaaaaac attgccacct tttttacaac caaaacaatg acaaatttgt ttatcttcag
    19141 aaactgtaaa tgaaggtgtc ttttcatcat gaaaaggaca caaacctata taattgcgtc
    19201 ctctcttttc taattttaca tattcactta ccaagtctaa aatgtcggtt ttatctttta
    19261 tttcattaat gatcgattga tctattcgca aattaaatca cctattaagc attagttata
    19321 ctattatacc taattttcga caaaaatgaa atcatttcga tttattttgt tcaatataat
    19381 ggattatatc gtttgctgtt tcttcgattg ctttttgaga aacatcaatg acaggacatc
    19441 caatttcact tacgatttct tcaaagtaat tcaattcttc ttgaattcgt gcttctgttg
    19501 catatcgagc tgtgtcacct agtcctaatt gttttagtcg ctcttttcta atgcgattta
    19561 atttttcttc acttatttta agtgcgatac atttctttgg attaatatca tataagccat
    19621 ctggcggtgt cacttctggt acaatcggta cattcataac tttgtaactc ttatgcgcta
    19681 aatactgaga taatggtgtc tttgaagttc tcgaaatacc aagtaaaaca atatcagctt
    19741 taggtaatcc tttaggatct ttaccatcat catatttaac tgcaaactct atcgcatcaa
    19801 ttttcttgaa atatgcatca tctaatctat gaacgatacc tggctcatta taaggttttt
    19861 cttcaaccga agcagataat aaatccatta atggccccat gatatcgaca gacttcaatt
    19921 ggaattctgc tactttctca ctcatatatt gcttcatttc aggtttaata agtgtataaa
    19981 caatgatagc atttgtatct tttgcaactt gaatcacttc atcaacatct tcaaaagatt
    20041 caatatatgg atatcttaat aattcatttt tacattgctt aggattgaat tgtgaaatac
    20101 ctgccctagc aactaactct gccgtttcac ctatagaatc tgaagctacg ataattttaa
    20161 ttttttccat tacttctcac ctattcttta tataatgcta ctaataactt agctattgtt
    20221 gttttggaaa ttcttccaat tacttcatac ttttgattat ctttttttct tacaattgga
    20281 atcgaatcaa tttctttttc aatcattcta tctgctgcgt atatgactaa ttcgctttcc
    20341 tctaaataag tgacattagg catacgtgtc atatttacac tgataggtac tttatgaata
    20401 tctgctccaa tcattgaagc tcttaataaa tcttttcttg aacacacacc aacaaaatcg
    20461 ttatcttcat taataataaa taatgtactt acatcttcta aaaaaattgt acaaatagcg
    20521 tcataaactg ttgtattctc tcttagcaca acaggttgag acatatagtc cttaacctca
    20581 aattgtcgaa gtttttcatt aaaaaatttg ccttttgatt tacctgaata ataatatcca
    20641 actcggggac gcgcttctaa aaaacctgac attgttaata tcgctaaatc tggtctaagc
    20701 gttgctcttg ttaaattcaa cttatctgct atttgttcac cagtaatggg tcctttagtt
    20761 ttaacaattt cgatgattcg ttcttgtctt tgactgagtt ctataggtct tcaccccttt
    20821 tttattcacc tattattata ccgatatttc tttcatgtca caaacacatt aattttactt
    20881 gcctttaaat aatctatcaa ttagaataaa cgtacagcga gttaaggata gtgtaagctt
    20941 aacaataaga ttggcgcaac gaatcatttt aaaataaaag cgagtgacta cactaatttg
    21001 ggtggaaccg cgggttaaat acgtaactta ttttgtatta tatacaattt aaaatattaa
    21061 ctcgtcccat ggaagtaaag catatttact atgctgttat tttccatggg gcgttttcat
    21121 gtatgaggag aggtaattat ggcaaaagat atggatacaa ttgtttcatt agcaaaacac
    21181 agaggttttg tgttccctgg tagtgatatt tacggtggtt tatcaaacac atgggattat
    21241 ggtcctttag gtgttgaatt aaagaataat gttaaaaaag cttggtggca aaaattcatt
    21301 acacaatcac cgtttaacgt tggtatcgat gctgcaatct taatgaatcc aaaagtatgg
    21361 gaagcttcag gacacttaaa caacttcaac gacccaatga ttgataataa agatagtaaa
    21421 attcgatatc gcgctgataa attaattgaa gattatatgc aagatgttaa aggtgatgaa
    21481 aacttcattg ccgatggttt aagttttgaa caaatgaaaa aaattattga cgatgaaggt
    21541 attgtttgtc ctgtaagtaa aactgctaac tggactgaaa ttcgccaatt caatttaatg
    21601 tttaaaacat tccaaggtgt aactgaagat tctacaaatg aaattttctt acgtcctgaa
    21661 acagcacaag gtatttttgt aaactataaa aacgtgcaac gttcaatgcg taaaaaatta
    21721 ccatttggta tcggtcaaat tggtaaatca ttccgtaatg aaatcactcc aggtaacttc
    21781 attttcagaa caagagaatt tgaacaaatg gaacttgaat tcttctgtaa acctggagaa
    21841 gaaatcgaat ggcaaaatta ttggaaaact tttgcaagtg actggttaac aagcttaaat
    21901 atgagcagtg aaaatatgcg tttacgtgat catgatgaag atgaattatc tcattactca
    21961 aatgcaacaa ctgatattga atataaattc ccatttggtt ggggtgagtt atggggtatc
    22021 gcaagtcgta cagacttcga cttacgtaaa catgctgaac actctggtga agatttcaga
    22081 taccatgatc cagaaacgaa cgaaaaatat attccatatt gtatcgagcc atcacttggt
    22141 gcagatcgtg taacattagc tttcttatgt gatgcatatg atgaagaagg cgttgaaggt
    22201 agtaaagatg cacgtacagt tttacacttc catcctgcat tagcaccata taaagcagcg
    22261 attttacctt taagtaagaa attatctggc gaagcgatta agatttttga gcaattaagt
    22321 tctaaattct caatcgattt cgatgaatca caatctatcg gtaaaagata ccgtcgtcaa
    22381 gatgaaatcg gtacacctta ttgtgtaaca ttcgactttg attcattaga agataatcaa
    22441 gttacagtac gtgacagaga ttcaatggaa caagttcgta tgccaatctc agagttagaa
    22501 gctttcttaa ctgaaaaaac aaaattctaa ttaaaaagac tttccaccat atgtggtggc
    22561 tatataattt ataaaaagag atactggtta gtaaaattgc cagtatctct tttctattca
    22621 aaaaggtatt caaataaaca catctatgtg gctacttgaa taccttttat tattttatgt
    22681 tattgttcca atctttttaa ttggttgatt agtttctgac ttttaaaaaa catacctgca
    22741 tattcacgat ataacattaa aatgatatct gacatttcat caataatttc ttgatggata
    22801 ttcaatgaat tcattttatc tatcggtaat ttttgtaata catctaataa atatagtgtt
    22861 ttattcgata atataactgc atgtacatct ttagaagcct cttgccttga aatcgcaccg
    22921 tcaaacttaa aactataacc tattaaatct gcttgtgtgt cattaccact cacagcacag
    22981 cgattaaatg atgcagtaaa accaaatcgt ttcatgcact ttaacataac tacgactgac
    23041 attaactgtg cagatgtacc tgattctatt ttttcaagaa caaattgtaa taattgatag
    23101 ttatatggtg caatgtcacc ttcatccatt gagcgctcaa tagtttcagc tgccagagcg
    23161 gcataactgc ttacgtaaag gtccatttgt aatttataat gttgactaat aacatctaca
    23221 gaatttaacg ttcccatacc tcgccactga ttgtaaataa acaaaccata aacaaacaat
    23281 tgcgtttgcg cttgtaaacc cgtcttaact tttttagcac gccttgccat aagtggtact
    23341 tttgcaccat gctcatttaa aatcgtgata attttatcag attcaccata atcaactgct
    23401 ttgatgataa tccctttttg gcgcattaac aattatcttc accactttta agattaatct
    23461 tggtcttcaa cataaccaat ttggcgaata aagttaactt tgtttcgcca gtctctttga
    23521 actttgaccc ataattctaa gtaaacttta gagcctagaa gcatttctat atcacgtctc
    23581 gcacgttttc ctacttcttt taacttttta ccgccttttc caatgacaat tcctttttgc
    23641 gaatctcttt caacatatat agttgcttcg atatgaacac gatcttcgct ttctttaacc
    23701 atacggtcca cattaacacc aatcgcatga gggatttctt cacttgtaag atgaaggatt
    23761 ttttcacgaa tgatttcacc cactacaaat tgttcaggat ggtctgaaat ttgatcatct
    23821 ggataatatt taggtccttc gggtaaatac gtctttaaaa catcaataaa atgatcgaca
    23881 tttagccctt ctaatgctga aataggtaca atctctgtaa agtccatata actttgatat
    23941 tcttcaatct ttggcattaa ttcatctgga tgcactaaat ctattttatt taatactaaa
    24001 aatactggtg tcttaacatt tttcaacatt tctataatat attcatcgcc tcgtccaatt
    24061 tcctcattgg cattaaccat aaacatgatt gcatctatct cagataatgt atttttagcg
    24121 actttcatca tatagtcacc taatttgtgt ttaggtttat gaatacctgg cgtatcaatg
    24181 aatataattt gcgcgtcatc tcttgtcata acaccttgaa ttttatttct agttgtttga
    24241 gctttatcgg acatgattgc tattttatgg ccgatcactc tattaacaaa tgttgacttt
    24301 cctacatttg gtctacctat aattgaaaca aatcctgatt tatgttctgt catttattct
    24361 aaatcctttc ctgaaaatcc aaatggtagt aactctgcga ctgtcatcat aaccatatct
    24421 cctttatgat ttgtcatata cacaggcata tcatcatcac ataattcctt caaaacttga
    24481 cgacatgcac cacaaggtga tgacggttta tctgcatcta cggttacagt tattgattca
    24541 aaatcacctg gtctgtatcc ttgagaaatt gccgatacca aactagctcg ttcagcacat
    24601 atcgataatg gataagaagc attttctaca ttggtaccat aaaaagttct accgtctttc
    24661 gtttttaaat aagcccctac tttaaattga ctgtatggcg aatatgattc ttgttgtgct
    24721 tttctaactt cttgaaaata atgaggttga taactcatat atgcctccct aaaataacgc
    24781 tataaaatgt ggtaaaaata ctattaaacc tataataaat gctaatattg aaactataag
    24841 tacactaaaa gccgcaatat ctttagcgta tttagctaaa tcatgatatt caacggtcac
    24901 taaatcgaca acatattcaa tagcagtgtt taaagcttca acagtgagaa ctaatgcaat
    24961 agcaatgagt ataaatatcc actcaatccg attaatattt agtacgagac caaagacaat
    25021 agcaacaatc attgcaaaca catgtaaaag aaatttatag tctttttgaa ttaagatttt
    25081 cagcccatca agtgcatatt taaacctttt cataattagt ctcgtgttaa tccatatgcg
    25141 tttaatattg tatcttgtcg accaaacatt tccttttcat ccgcttcagt catatgatca
    25201 taacctaata gatgcaaaaa tccatgtaat gctaaaaatc ctaattctcg ttcaaaagaa
    25261 tgtccgtaat tgtttgcttg ttcttgcgct acatccgtac agataattat atcccctaaa
    25321 acacgtggta tatcaagacc actaaaatca atctctggct catcttcttc taaagcaaat
    25381 gagattacat ctgtaacttt atctttatct ctatatgttc gattaatttc ttgtatttct
    25441 tgtttatcta caaatgtaac agaaagctca gcatcgtctt ctatatgctc ttctttttta
    25501 gcaaattcta ataaatcttc aatttgttta taccaagcat ctttaactaa gcctgtgtga
    25561 tcgctaaaat ctatcgtaaa catttaattc tctccttcat aatgttcaat gatcttactt
    25621 accaatggat gtctgactac atcgctctga tctaatttca atatacttat acctttaacg
    25681 ttgtgtaacc tactgaccgc ttccttaagt ccacttttaa cacctttagg taaatcgatt
    25741 tgagtttggt caccagtaac taccattttt gagccaaaac ctagtcttgt taaaaacatt
    25801 ttcatttgcg catgtgtcgt attctgcgcc tcatcaagaa ttacaaatgc atcttctaat
    25861 gttcgtccgc gcatatatgc aagtggcgct atttcgataa tgcctctttc aataaatcgc
    25921 tctgtttgtt cacgcccaag aacagtatat agaccatcat ataaaggtct taaatatgga
    25981 tctacctttt ctttcaaatc tcctggtaaa aatccaagtg actctcctgc ttcaacagca
    26041 ggtcttgtta atacaatacg tttaacagca cctttacgga gttgctttgc tgcataaact
    26101 acagctaaga atgtcttacc tgtaccagca ggacctatac caaatactaa atcattattt
    26161 ttcatggcat taacatatat acgttgcccc atcgttttcg cacgaatcgt cttaccaaat
    26221 gcatctttag ttatctcttc atcatataaa tctaacagat gttgaattgt gttattatgc
    26281 gccattttaa tagctgcttc aacatcttta attgtaatat tattacctaa atcaataacc
    26341 ttcagcaaat tgattaatac tgattccgct ttttctacgt tttctatttt tgtaccttta
    26401 acggcaactt cttgtcctct tgcatggatg acaacatcga aactctcttc aattgctttt
    26461 aaatgttcat cattatttcc aattaaagct tgagattggt tcatatcgtc tatttgtata
    26521 attccaggca tacacgcgct ccttttcata tatcatttat attcattcta tgatgcttta
    26581 atatctactt aattatatca tgcaattgat tgatttaaac atctttgcat tgacttactc
    26641 atttttcttt aatttaaaat aatcatttat acttttcacc ttgtcataaa agcagattca
    26701 attttagact aatcaatttt ttgctgtaat ttaaataact aatatttctc gaaaaattct
    26761 ctttgttttt taatttttct taacgtaaaa aaacaagcaa ctaccattac gatagtgctt
    26821 gttttcaaaa aattttataa ttgttttggt ttagctaaaa tttctgacca tatcatacca
    26881 ttgattactt catctttatc aaattggaac gctgatttag tcaaatcttt ttcgacattt
    26941 tgagaattaa gcagctgctt taatttcaaa cgttttgtac gttcagataa gtatttatcc
    27001 tcaataatat ctctagcacg tttttccatt ttagcaattt gcttttcttt ttctctatca
    27061 atgtcagtac gaatcgtttt aatatcatct ctaagtgatt tttctaattg acgtctaatt
    27121 tcgtcagaat tatcttgtct agaaggtcta tgtttctgct ccaccggttc tgctttaggc
    27181 actgtgattg gtttctctgt catcggtttt gtttcttgtt gttttttagg tgccataggt
    27241 tctacaacag gtttcgattt aggctcttcc tttggaagtt catcgaataa aggtggtaac
    27301 gtatcatcat attttcgttt cgatgatttc ttttcttctt catttaattc ttcacttatt
    27361 tctttaaacg ttcgctcaat ttcttcaaaa aagccacctt tttttggttc attatcggta
    27421 gatgtttttt gaggtggctt ttgattttgt ctatctttat gactattttc gcgcatagta
    27481 gtaatgatag aaatgatcac tgatatgaca aaaattagaa taccgacact catttaatca
    27541 cctctcgact taatgttcag gtgactcatc atcactttga tcagttcgtt tattaattgc
    27601 atttctcatg cctgtatcag cttcgatatt tttcaaatta taataatctt taacactgat
    27661 attacctgaa cgtaatgctt cagccatagc taatggtact tcagattcgg cttcaactac
    27721 tttagcatgc atttcttgta cacgcgcttt catttcttgc tcagttgcta cagccatagc
    27781 tctacgttct tcagcttttg cttgtgcaat atttttgtct gctaatgctt gttcagtttg
    27841 taagtctgca ccaatatttt tactaatatc aacgtcagca atatcaattg ataaaatttc
    27901 aaatgcagta cctgaatcta aacctttgct taaaactgtt ttagaaatat tatctgggtt
    27961 ttcaagtact tctgtatgat gcttactaga accaattgtt gaaacgatac cttcaccaac
    28021 acgtgcgatg attgtttctt caccagcacc accaacaagt cgagcaatat tagctctaac
    28081 tgtgatacga gctttggctt tcacttcaat accgttcatt gctacacctg cgataaatgg
    28141 tgtttcaatg actttaggat taacagacat ttgaaccgct tctaatacgt cacgtcctgc
    28201 aaggtcaatt gcagcagcac gttcgaaagg aagatcaatg tcagcacgtt gtgcagcaat
    28261 attagcgtca acaactctgt caacatttcc tcctgctaga taatgcgatt ctaattggtt
    28321 tgttgttaat gctagtcctg ctttgtgcgc tttaattaat ggcgctataa cttttcttgg
    28381 agatacacga cgtaaacgca taccaaccaa tgtacctata ccaacatgaa cgccagctgc
    28441 taacgctgaa atccataaac caatgggtac aaatgagaat aaaataagta atgcaactac
    28501 tataataact gctattacga taaaacttaa actaaacatg gtatcgctcc ttttaattaa
    28561 tctacttccc tcacaactac tcttgttcct tcaacttcaa ggatttttac cgttttattg
    28621 cgcaaaataa agttgccatc tgaaacagca tcaatacgtt cattttcaca aaaaataatc
    28681 cctgcaggtc gaagatctgt aactgtttga gcagtctttc ctacgaggtg cgagcggtta
    28741 tcatgagaat tgtaacctga ctcagaatta gttgaatctt ttaagataac tttatccaaa
    28801 aacggaatct ttctgttgaa aatcttcact aatatcaccc attctacaat cgttaaaatc
    28861 aaggcaacga taacattcgc aagcataaat agcaaattat caccgagcgt tgttatgctt
    28921 atagttatca gtatcatgcc aataatacca attactgcac caactacaaa taattcaatt
    28981 acaactaata taacgccaat tgagaatatt aagatagaat gcatattgac atttccttgg
    29041 attagaaatc ccaaaaataa aataagtaat gataatgtgg cgataatacc agctgcattg
    29101 atttttttag agtaaagttg atatacaaat cctaagaatg tcaaacaggt taatattaac
    29161 gtaaaaatag gttgaacaat tatattgcta acttgttcaa cccaagtatc tccagccgtt
    29221 gattccaaga tagttgtcat ttgtaaaaaa ttattataac tcacaatttc acctcgccct
    29281 cacattaatt atacatgaaa ttgaatatac tttatagtta taagaaccta tagaatatca
    29341 atttgattat ttataaacta tataactttt tttaattaaa aattga
//
LOCUS       DAKTIO010000014        28993 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.014, whole genome shotgun sequence.
ACCESSION   DAKTIO010000014 DAKTIO010000000
VERSION     DAKTIO010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 28993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28993
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.014"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..539)
                     /gene="hpt"
                     /locus_tag="O0293_000476"
     CDS             complement(<1..539)
                     /gene="hpt"
                     /locus_tag="O0293_000476"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCY0279386.1"
                     /translation="MHNDLKEVLLTEEDIQNICKELGAQLTKDYQGKPLVCVGILKGS
                     AMFMSDLIKRIDTHLSIDFMDVSSYHGGTESTGEVQIIKDLGSSIENKDVLIIEDILE
                     TGTTLKSITELLQSRKVNSLEIVTLLDKPNRRKADIEAKYVGKKIPDEFVVGYGLDYR
                     ELYRNLPYIGTLKPEVYSN"
     gene            complement(544..1839)
                     /gene="tilS"
                     /locus_tag="O0293_000477"
     CDS             complement(544..1839)
                     /gene="tilS"
                     /locus_tag="O0293_000477"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCY0279387.1"
                     /translation="MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTC
                     LHVNHGVRSASIEEARFLEAYCERHHIDLHIKKLDLSHSLDRNNSIQNEARIKRYEWF
                     DEMMNVLEADVLLTAHHLDDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLA
                     VSKKEIKQFQERYHIPYFEDESNKDNKYVRNDIRNRIIPAIDENNQLKVSHLLKLKQW
                     HDEQYDILQYSAKQFIQEFVKFDEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYEL
                     FNISAKTYEEWFKQFSSKKAQFSINLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIKK
                     PGNYIFNKYRLEIHSNLPKCLFPLTVRTRQSGDTFKLNGRDGYKKVNRLFIDCKVPQW
                     VRDQMPIVLDKQQRIIAVGDLYQQQTIKKWIIISKNGDE"
     gene            complement(2019..2420)
                     /locus_tag="O0293_000478"
     CDS             complement(2019..2420)
                     /locus_tag="O0293_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HCY0279388.1"
                     /translation="MSIEVGNKLKGKVTGIKKFGAFVELPEGKSGLVHISEVADNYVE
                     NVEEHLSVGDEVDVKVLSIADDGKISLSIKKAKDRPRRQHTSKPSHQKPVQKAEDFEK
                     KLSNFLKDSEDKLTSIKRQTESRRGGKGSRR"
     gene            complement(2525..2917)
                     /locus_tag="O0293_000479"
     CDS             complement(2525..2917)
                     /locus_tag="O0293_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HCY0279389.1"
                     /translation="MKNKVEHIENQYTSQENKKKQRQKMKMRVVRRRITVFAGVLLAI
                     IVVLSILLVVQKHRNDIDAQERKAKEAQFQKQQNEEIALKEKLNNLNDKDYIEKIARD
                     DYYLSNKGEVIFRLPEDKDSSSSKSSKK"
     gene            complement(2935..3198)
                     /locus_tag="O0293_000480"
     CDS             complement(2935..3198)
                     /locus_tag="O0293_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HCY0279390.1"
                     /translation="MRLDKYLKVSRLIKRRTLAKEVSDQGRITINGNVAKAGSDVKVE
                     DVLTIRFGQKLVTVKVTALNEHASKDNAKGMYEIIEERRLEEA"
     gene            complement(3195..4388)
                     /locus_tag="O0293_000481"
     CDS             complement(3195..4388)
                     /locus_tag="O0293_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCY0279391.1"
                     /translation="MAHTITIVGLGNYGIDDLPLGIYKFLKTQDKVYARTLDHPVIES
                     LQDELTFQSFDHVYEAHNQFEDVYIDIVAQLVEAANEKDIVYAVPGHPRVAETTTVKL
                     LALAKDNTDIDVKVLGGKSFIDDVFEAVNVDPNDGFTLLDATSLQEVTLNVRTHTLIT
                     QVYSAMVAANLKITLMERYPDDYPVQIVTGARSDGADNVVTCPLYELDHDENAFNNLT
                     SVFVPKIITSTYLYHDFDFATEVIDTLVDEDKGCPWDKVQTHETLKRYLLEETFELFE
                     AIDNEDDWHMIEELGDILLQVLLHTSIGKKEGYIDIKEVITSLNAKMIRRHPHIFGDA
                     NAETIDDLKEIWSKAKDAEGKQPRVIFEKVFAEHFLNLYEKTKDKSFDEAALKQWLEK
                     GESNT"
     gene            complement(4388..5914)
                     /locus_tag="O0293_000482"
     CDS             complement(4388..5914)
                     /locus_tag="O0293_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCY0279392.1"
                     /translation="MKHKEAFNGVVVLTAALIVIKILSAVYRIPYQNILGDTGLYAYQ
                     QVYPIVALGMILSMNAIPSAITQNIGKYHSDEAYAKAVAYIQLVGILLFIAIFVFANN
                     IAHMMGDGHLTPMIQAASLSFIFIGMLGVLRGYYQSANNMTVPAISQVIEQVIRVGII
                     IVTIVIFVDRGWTIYEAGTIAILASTIGFLGSSIYLVAHRPFKFKMVNNTAKIVWKQF
                     ALSVLIFAISQLIVILWQVIDSVTIIKSLQAIRVPFDVAITEKGVYDRGASFIQMGLI
                     VTTTFSFALIPLLSDAIKMNNQVLMNRYANASLKITILISTAAGIGLINLLPLMNGVF
                     FKTNDLTLTLSVYMITVICVSLIMMDMALLQAQHAVRPIFVGMTAGLVIKFILNIILI
                     RLSGIIGASISTVVSLIIFGTIIHIAVTRKYHLYAMRRFFINVVLGMVFMSIVVQCVL
                     NIVTTHGRITGLIELLCAAVLGIIALFFYIFRFNVLTYKELTYLPFGSKLYQIKKGRR
                     "
     gene            complement(5904..9410)
                     /gene="mfd"
                     /locus_tag="O0293_000483"
     CDS             complement(5904..9410)
                     /gene="mfd"
                     /locus_tag="O0293_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCY0279393.1"
                     /translation="MTILTTLIKEDNHFQDLNQVFGQANTLVTGLSPSAKVTMIAEKY
                     AQSNQQLLLITNNLYQADKLETDLLQFIDAEELYKYPVQDIMTEEFSTQSPQLMSERI
                     RTLTALAQGKKGLFIVPLNGLKKWLTPVEMWQNHQMTLRVGEDIDVDQFLNKLVNMGY
                     KRESVVSHIGEFSLRGGIIDIFPLIGEPIRIELFDTEIDSIRDFDVETQRSKDNVEEV
                     DITTASDYIITEEVISHLKEELKTAYENTRPKIDKSVRNDLKETYESFKLFESTYFDH
                     QILRRLVAFMYETPSTIIEYFQKDAIIAVDEFNRIKETEESLTVESDSFISNIIESGN
                     GFIGQSFIKYDDFETLIEGYPVTYFSLFATTMPIKLNHIIKFSCKPVQQFYGQYDIMR
                     SEFQRYVNQNYHIVVLVETETKVERMQAMLSEMHIPSITKLHRSMSSGQAVIIEGSLS
                     EGFELPDMGLVVITERELFKSKQKKQRKRTKAISNAEKIKSYQDLNVGDYIVHVHHGV
                     GRYLGVETLEVGQTHRDYIKLQYKGTDQLFVPVDQMDQVQKYVASEDKTPKLNKLGGS
                     EWKKTKAKVQQSVEDIAEELIDLYKEREMAEGYQYGEDTAEQTTFELDFPYELTPDQA
                     KSIDEIKDDMQKSRPMDRLLCGDVGYGKTEVAVRAAFKAVMEGKQVAFLVPTTILAQQ
                     HYETLIERMQDFPVEIQLMSRFRTPKEIKQTKEGLKTGFVDIVVGTHKLLSKDIQYKD
                     LGLLIVDEEQRFGVRHKERIKTLKHNVDVLTLTATPIPRTLHMSMLGVRDLSVIETPP
                     ENRFPVQTYVLEQNMSFIKEALERELSRDGQVFYLYNKVQSIYEKREQLQMLMPDANI
                     AVAHGQMTERDLEETMLSFINNEYDILVTTTIIETGVDVPNANTLIIEDADRFGLSQL
                     YQLRGRVGRSSRIGYAYFLHPANKVLTETAEDRLQAIKEFTELGSGFKIAMRDLNIRG
                     AGNLLGKQQHGFIDTVGFDLYSQMLEEAVNEKRGIKEPESEVPEVEVDLNLDAYLPTE
                     YIANEQAKIEIYKKLRKTETFDQIIDIKDELIDRFNDYPVEVARLLDIVEIKVHALHS
                     GITLIKDKGKIIDIHLSVKATENIDGEVLFKATQPLGRTMKVGVQNNAMTITLTKQNQ
                     WLDSLKFLVKCIEESMRISDEA"
     gene            complement(9410..9982)
                     /locus_tag="O0293_000484"
     CDS             complement(9410..9982)
                     /locus_tag="O0293_000484"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCY0279394.1"
                     /translation="MKCIVGLGNIGKRFELTRHNIGFEVVDYILEKNNFSLDKQKFKG
                     AYTIERMNGDKVLFIEPMTMMNLSGEAVAPIMDYYNVNPEDLIVLYDDLDLEQGQVRL
                     RQKGSAGGHNGMKSIIKMLGTDQFKRIRIGVGRPTNGMTVPDYVLQRFSNDEMVTMEK
                     VIEHAARAIEKFVETSRFDHVMNEFNGEVK"
     gene            complement(10293..10946)
                     /locus_tag="O0293_000485"
     CDS             complement(10293..10946)
                     /locus_tag="O0293_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HCY0279395.1"
                     /translation="MASLKSIIRQGKQTRSDLKQLRKSGKVPAVVYGYGTKNVSVKVD
                     EVEFIKVIREVGRNGVIELGVGSKTIKVMVADYQFDPLKNQITHIDFLAINMSEERTV
                     EVPVQLVGEAVGAKEGGVVEQPLFNLEVTATPDNIPEAIEVDITELNINDSLTVADVK
                     VTGDFKIENDSAESVVTVVAPTEEPTEEEIEAMEGEQQTEEPEVVGESKEDEEKTEE"
     gene            complement(11096..12061)
                     /locus_tag="O0293_000486"
     CDS             complement(11096..12061)
                     /locus_tag="O0293_000486"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCY0279396.1"
                     /translation="MLNNEYKNSSLKIFSLKGNEALAQEVADQVGIELGKCSVKRFSD
                     GEIQINIEESIRGCDVFIIQPTSYPVNLHLMELLIMIDACKRASAATINIVVPYYGYA
                     RQDRKARSREPITAKLVANLIETAGATRMIALDLHAPQIQGFFDIPIDHLMGVPILAK
                     HFKDDPNINPEECVVVSPDHGGVTRARKLADILKTPIAIIDKRRPRPNVAEVMNIVGE
                     IEGRTAIIIDDIIDTAGTITLAAQALKDKGAKEVYACCTHPVLSGPAKERIENSAIKE
                     LIVTNSIHLDEDRKPSNTKELSVAGLIAQAIIRVYERESVSVLFD"
     gene            complement(12208..13560)
                     /gene="glmU"
                     /locus_tag="O0293_000487"
     CDS             complement(12208..13560)
                     /gene="glmU"
                     /locus_tag="O0293_000487"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCY0279397.1"
                     /translation="MRRHAIILAAGKGTRMKSKKYKVLHEVAGKPMVEHVLESVKGSG
                     VDQVVTIVGHGAESVKGHLGERSLYSFQEEQLGTAHAVQMAKSHLEDKEGTTIVVCGD
                     TPLITKETLETLIAHHEDANAQATVLSASIQQPYGYGRIVRNASGRLERIVEEKDATQ
                     AEKDINEISSGIFAFNNKTLFEKLTQVKNDNAQGEYYLPDVLSLILNDGGIVEVYRTN
                     DVEEIMGVNDRVMLSQAEKAMQRRTNHYHMLNGVTIIDPDSTYIGPDVTIGSDTVIEP
                     GVRINGRTEIGEDVVIGQYSEINNSTIENGACIQQSVVNDASVGANTKVGPFAQLRPG
                     AQLGADVKVGNFVEIKKADLKDGAKVSHLSYIGDAVIGERTNIGCGTITVNYDGENKF
                     KTIVGKDSFVGCNVNLVAPVTIGDDVLVAAGSTITDDVPNDSLAVARARQTTKEGYRK
                     "
     gene            complement(13583..13714)
                     /locus_tag="O0293_000488"
     CDS             complement(13583..13714)
                     /locus_tag="O0293_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279398.1"
                     /translation="MRLNDARIFEVRKKLFLKLQRIKNNAFYMLKEYCRLNYNNDEV"
     gene            complement(13904..14206)
                     /gene="spoVG"
                     /locus_tag="O0293_000489"
     CDS             complement(13904..14206)
                     /gene="spoVG"
                     /locus_tag="O0293_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HCY0279399.1"
                     /translation="MKVTDVRLRKIQTDGRMKALVSITLDEAFVIHDLRVIEGNSGLF
                     VAMPSKRTPDGEFRDIAHPINSDMRQEIQDAVMKVYDETDEVVPDKNATSEDSEEA"
     gene            complement(14278..14658)
                     /locus_tag="O0293_000490"
     CDS             complement(14278..14658)
                     /locus_tag="O0293_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCY0279400.1"
                     /translation="MKIINTTRLPEALGPYSHATVVNGMVYTSGQIPLNIDGHIVSAD
                     VQAQTKQVLENLKVVLEEAGSDLNSVAKATIFIKDMNDFQKINEVYGQYFNEHKPARS
                     CVEVARLPKDVKVEIELVSKIKEL"
     gene            complement(14675..15499)
                     /gene="purR"
                     /locus_tag="O0293_000491"
     CDS             complement(14675..15499)
                     /gene="purR"
                     /locus_tag="O0293_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HCY0279401.1"
                     /translation="MRYKRSERIVFMTQYLMNHPNKLIPLTFFVKKFKQAKSSISEDV
                     QIIKNTFQKEKLGTVITTAGASGGVTYKPMMSKEEATEVVNEVITLLEEKERLLPGGY
                     LFLSDLVGNPSLLNKVGKLIASIYMEEKLDAVVTIATKGISLANAVANILNLPVVVIR
                     KDNKVTEGSTVSINYVSGSSRKIETMVLSKRTLAENSNVLVVDDFMRAGGSINGVMNL
                     MNEFKAHVKGVSVLVESKEVKQRLIEDYTSLVKLSDVDEYNQEFNVEPGNSLSKFS"
     gene            complement(15513..16361)
                     /locus_tag="O0293_000492"
     CDS             complement(15513..16361)
                     /locus_tag="O0293_000492"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCY0279402.1"
                     /translation="MIYETAPAKINFTLDTLFKRNDGYHEIEMIMTTVDLNDRLTFHK
                     RKDRKIVVEIEHNYVPSNHKNLAYRAAQLFIEQYQLKQGVTISIDKEIPVSAGLAGGS
                     ADAAATLRGLNRLFDIGASLEELALLGSKIGTDIPFCIYNKTALCTGRGEKIEFLNKP
                     PSAWVILAKPNLGISSPDIFKLINLDKRYDVHTKMCYEALENRDYQQLCQSLSNRLEP
                     ISVSKHPQIDKLKNNMLKSGADGALMSGSGPTVYGLARKESQAKNIYNAVNGCCNEVY
                     LVRLLG"
     gene            complement(16671..16934)
                     /locus_tag="O0293_000493"
     CDS             complement(16671..16934)
                     /locus_tag="O0293_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HCY0279403.1"
                     /translation="MPKSILDIKNSIDCHVGNRIVLKANGGRKKTIKRSGILKETYPS
                     VFIVELDQDKHNFERVSYTYTDVLTENVQVSFEEDNHHESIAH"
     gene            complement(17034..17927)
                     /gene="rsmA"
                     /locus_tag="O0293_000494"
     CDS             complement(17034..17927)
                     /gene="rsmA"
                     /locus_tag="O0293_000494"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCY0279404.1"
                     /translation="MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIID
                     ASDIDAQTGVIEIGPGMGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVIN
                     EDILKANIKEAVENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEV
                     GERLNAEVGSKAYGSLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLV
                     TVDNEEAFFKLAKAAFAQRRKTINNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGET
                     LSIQDFAKLYEEKKKFPQLEN"
     gene            complement(17938..18474)
                     /gene="rnmV"
                     /locus_tag="O0293_000495"
     CDS             complement(17938..18474)
                     /gene="rnmV"
                     /locus_tag="O0293_000495"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HCY0279405.1"
                     /translation="MKINEFIVVEGRDDTERVKRAVECDTIETNGSAINEQTLEVIRN
                     AQQSRGVIVLTDPDFPGDKIRSTITEHVKGVKHAYIDREKAKNKKGKIGVEHAELIDI
                     KEALMHVSSPFDEAYESIDKSVLIELGLIVGKDARRRREILSRKLRIGHSNGKQLLKK
                     LNAFGYTEADVRQALEDE"
     gene            complement(18641..19414)
                     /locus_tag="O0293_000496"
     CDS             complement(18641..19414)
                     /locus_tag="O0293_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCY0279406.1"
                     /translation="MLIDTHVHLNDEQYDDDLSEVITRAREAGVDRMFVVGFNKSTIE
                     RAMKLIDEYDFLYGIIGWHPVDAIDFTEEHLEWIESLAQHPKVIGIGEMGLDYHWDKS
                     PADVQKEVFRKQIALAKRLKLPIIIHNREATQDCIDILLEEHAEEVGGIMHSFSGSPE
                     IADIVTNKLNFYISLGGPVTFKNAKQPKEVAKHVSMERLLVETDAPYLSPHPYRGKRN
                     EPARVTLVAEQIAELKGLSYEEVCEQTTKNAEKLFNLNS"
     gene            complement(19445..21418)
                     /gene="metG"
                     /locus_tag="O0293_000497"
     CDS             complement(19445..21418)
                     /gene="metG"
                     /locus_tag="O0293_000497"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCY0279407.1"
                     /translation="MAKETFYITTPIYYPSGNLHIGHAYSTVAGDVIARYKRMQGYDV
                     RYLTGTDEHGQKIQEKAQKSGKTEIEYLDEMIAGIKQLWAKLEISNDDFIRTTEERHK
                     HVVEQVFERLLKQGDIYLGEYEGWYSVPDETYYTESQLVDPQYENGKIIGGKSPDSGH
                     EVELVKEESYFFNISKYTDRLLEFYDQNPDFIQPPSRKNEMINNFIKPGLADLAVSRT
                     SFNWGVHVPSNPKHVVYVWIDALVNYISALGYLSDDESLFNKYWPADIHLMAKEIVRF
                     HSIIWPILLMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPNILIDRYGLDATRYY
                     LMRELPFGSDGVFTPEAFVERTNFDLANDLGNLVNRTISMVNKYFDGELPAYQGPLHE
                     LDEEMEAMALETVKSYTESMESLQFSVALSTVWKFISRTNKYIDETTPWVLAKDDSQK
                     DMLGNVMAHLVENIRYAAVLLRPFLTHAPKEIFEQLNINNPQFMEFSSLEQYGVLNES
                     IMVTGQPKPIFPRLDSEAEIAYIKESMQPPATKEEKEEIPSKPQIDIKDFDKVEIKAA
                     TIIDAEHVKKSDKLLKIQVDLDSEQRQIVSGIAKFYTPDDIIGKKVAVVTNLKPAKLM
                     GQKSEGMILSAEKDGVLTLVSLPSAIPNGAVIK"
     gene            complement(21703..22542)
                     /gene="rsmI"
                     /locus_tag="O0293_000498"
     CDS             complement(21703..22542)
                     /gene="rsmI"
                     /locus_tag="O0293_000498"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCY0279408.1"
                     /translation="MAVLYLVGTPIGNLADITYRAVDVLKRVDMIACEDTRVTSKLCN
                     HYDIPTPLKSYHEHNKDKQTAFIIEQLELGLDVALVSDAGLPLISDPGYELVVAAREA
                     NIKVETVPGPNAGLTALMASGLPSYVYTFLGFLPRKEKEKSAVLEQRMHENSTLIIYE
                     SPHRVTDTLKTIAKIDATRQVSLGRELTKKFEQIVTDDVTQLQALIQQGDVPLKGEFV
                     ILIEGAKANNEISWFDDLSINEHVDHYIQTSQMKPKQAIKKVAEERQLKTNEVYNIYH
                     QIS"
     gene            complement(22544..22792)
                     /locus_tag="O0293_000499"
     CDS             complement(22544..22792)
                     /locus_tag="O0293_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCY0279409.1"
                     /translation="MDSHFVYIVKCSDGSLYTGYAKDVNARVEKHNRGQGAKYTKVRR
                     PVHLVYQEMYETKSEALKREYEIKTYTRQKKLGLIKER"
     gene            complement(22785..23510)
                     /locus_tag="O0293_000500"
     CDS             complement(22785..23510)
                     /locus_tag="O0293_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HCY0279410.1"
                     /translation="MLKENERFDQLIKEDFSIIQNDDVFSFSTDALLLGHFTKPRTKD
                     IVLDLCSGNGVIPLLLFAKHPRHIEGVEIQKTLVDMARRTFQFNDVDEYLTMHHMDLK
                     NVTKVFKPSQYTLVTCNPPYFKENQQHQHQKEAHKIARHEIMCTLEDCMIAARHLLKE
                     GGRLNMVHRAERLMDVLFEMRKVNIEPKKVVFIYSKVGKSAQTIVVEGRKGGNQGLEI
                     MPPFYIYNEDGNYSEEMKEVYYG"
     gene            complement(23784..24131)
                     /gene="yabA"
                     /locus_tag="O0293_000501"
     CDS             complement(23784..24131)
                     /gene="yabA"
                     /locus_tag="O0293_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HCY0279411.1"
                     /translation="MDRNEIFEKIMRLEMNVNQLSKETSELKALAVELVEENVALQLE
                     NDNLKKVLGNDEPTTIDTANSKPAKAVKKPLPSKDNLAILYGEGFHICKGELFGKHRH
                     GEDCLFCLEVLSD"
     gene            complement(24148..24951)
                     /locus_tag="O0293_000502"
     CDS             complement(24148..24951)
                     /locus_tag="O0293_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HCY0279412.1"
                     /translation="MPNVIGVQFQKAGKLEYYTPNDIQVDIEDWVVVESKRGIEIGIV
                     KNPLMDIAEEDVVLPLKNIIRIADDKDIDKFNCNERDAENALILCKDIVREQGLDMRL
                     VNCEYTLDKSKVIFNFTADDRIDFRKLVKILAQHLKTRIELRQIGVRDEAKLLGGIGP
                     CGRSLCCSTFLGDFEPVSIKMAKDQNLSLNPTKISGACGRLMCCLKYENDYYEEVRAQ
                     LPDIGEAIETPDGNGKVVALNILDISMQVKLEGHEQPLEYKLEEIETMH"
     gene            complement(24952..25878)
                     /locus_tag="O0293_000503"
     CDS             complement(24952..25878)
                     /locus_tag="O0293_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCY0279413.1"
                     /translation="MDEQQQLTNAYHSNKLSHAYLFEGDDAQTMKQVAINFAKLILCQ
                     TDSQCETKVSTYNHPDFMYISTTENAIKKEQVEQLVRHMNQLPIESTNKVYIIEDFEK
                     LTVQGENSILKFLEEPPDNTIAILLSTKPEQILDTIHSRCQHVYFKPIDKEKFINRLV
                     EQNMSKPVAEMISTYTTQIDNAMALNEEFDLLALRKSVIRWCELLLTNKPMALIGIID
                     LLKQAKNKKLQSLTIAAVNGFFEDIIHTKVNVEDKQIYSDLKNDIDQYAQKLSFNQLI
                     LMFDQLTEAHKKLNQNVNPTLVFEQIVIKGVS"
     gene            complement(26092..26421)
                     /locus_tag="O0293_000504"
     CDS             complement(26092..26421)
                     /locus_tag="O0293_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HCY0279414.1"
                     /translation="MKMIIAIVQDQDSQELADQLVKNNFRATKLATTGGFLRAGNTTF
                     LCGVNDDRVDEILSVINQTCGNREQLVSPITPMGGSADSYIPYPVEVEVGGATVFVMP
                     VDAFHQF"
     gene            complement(26449..27066)
                     /locus_tag="O0293_000505"
     CDS             complement(26449..27066)
                     /locus_tag="O0293_000505"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCY0279415.1"
                     /translation="MSAFITFEGPEGSGKTTVINEVYHRLVKDYDVIMTREPGGVPTG
                     EEIRKIVLEGNDMDIRTEAMLFAASRREHLVLKVIPALKEGKVVLCDRYIDSSLAYQG
                     YARGIGVEEVRALNEFAINGLYPDLTIYLNVSAEVGRERIIKNSRDQNRLDQEDLKFH
                     EKVIEGYQEIIHNESQRFKSVNADQPLENVVEDTYQTIIKYLEKI"
     gene            complement(27068..28405)
                     /locus_tag="O0293_000506"
     CDS             complement(27068..28405)
                     /locus_tag="O0293_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HCY0279416.1"
                     /translation="MKQPILNKLESLNQEEAISLHVPGHKNMTIGHLSQLSMTMDKTE
                     IPGLDDLHHPEEVILESMKQVEKHSDYDAYFLVNGTTSGILSVIQSFSQKKGDILMAR
                     NVHKSVLHALDISQQEGHFIETHQSPLTNHYNKVNLSRLNNDGHKLAVLTYPNYYGET
                     FNVEEVIKFLHQLNIPVLIDEAHGAHFGLQGFPDSTLNYQADYVVQSFHKTLPALTMG
                     SVLYIHKNAPYRETIIEYLSYFQTSSPSYLIMASLESAAEFYKTYDSTVFFDKRAQLI
                     ECLEKKGFEMLQVDDPLKLLIKYEGFTGHDIQNWFMNAHIYLELADDYQALAILPLWH
                     HDDTYLFDSLLRKIEDMILPKKSVSKVKQTQLLTTEGNYKPKRFEYVTWCDLKKAKGK
                     VLARHIVPYPPGIPIIFKGETITENMIELVNEYLETGMIVEGIKNNKILVEDE"
BASE COUNT         8807 a         5782 c         3891 g        10513 t
ORIGIN      
        1 taatttgaat acacttcagg ttttaacgta ccgatatatg gtaagtttcg gtataattca
       61 cgataatcta aaccgtaacc aacaacaaat tcatctggta tttttttacc tacatactta
      121 gcttcaatgt ccgctttacg acggtttggt ttatctaata aagtaactat ttctaatgaa
      181 ttaacttttc tagattgtaa taattcagta attgacttaa gtgtagtacc agtctctaag
      241 atatcttcaa taattaatac gtctttattt tcaatagaag aacctaaatc tttaatgatt
      301 tgaacttcac cagttgactc agtgcctccg tgataactag aaacatccat gaaatcaatt
      361 gataaatggg tatcaattcg tttaattaaa tctgacataa acattgctga gccttttaag
      421 atacccacgc atactaatgg tttaccttga taatcctttg ttaattgtgc tcccaattcc
      481 ttacagatat tttgaatatc ttcttcagtt aacaatactt ctttcaaatc attatgcata
      541 acgctattca tctccatttt tactaattat aatccatttt tttattgttt gttgttgata
      601 taaatctcct accgcaataa tgcgctgttg tttatccaat acgattggca tttgatcccg
      661 aacccactgt ggcactttac aatctataaa caggcgattc actttcttat aaccatcgcg
      721 cccattcagt ttaaatgtat cgccactttg tcgtgttctc actgtaagcg gaaataaaca
      781 ttttggtaaa ttagaatgta tctctaatcg atatttgtta aaaatataat tacctggctt
      841 tttaattgtt tgaactctaa aatatgtatc gccattattt ttagccatta ttattaattt
      901 accatatgcg atttgaatta tccatttatc cgtgagatta atactgaatt gtgctttctt
      961 actactaaat tgtttaaacc actcttcgta tgttttagca ctaatattaa acaactcata
     1021 atactttgat aataggcagt ccaacacaac catctttaat gagtttggta agttattaaa
     1081 agcttgtcta gaaacctcta aatattttga ctgttcatca aacttcacaa attcttgaat
     1141 aaattgttta gctgaatatt gcaaaatatc atattgttca tcatgccatt gttttaattt
     1201 taataaatgc gatactttaa gttgattatt ttcatcaata gctggaataa ttctattacg
     1261 aatatcattt ctaacatatt tgttatcttt attagattca tcttcaaaat atggaatatg
     1321 atatctctct tggaattgtt ttatttcttt tttagagaca gctaaaagtg gtcgataaat
     1381 ctgataacct tttcgcttcg ataactcatc aaatcctagt ttattacgtg ttgatttccc
     1441 attaaaaata cgatacataa tagtttctaa ttgatcgtcc aaatgatgcg ccgttagcaa
     1501 tacatccgct tctaatacat tcatcatttc atcaaaccat tcgtaacgtt taattcgagc
     1561 ttcattctga atgctgttat ttcggtcgag actatgcgac aaatctaact ttttgatatg
     1621 taaatcgatg tgatgacgtt cgcagtatgc ttctaaaaat ctggcttcct caattgaagc
     1681 tgacctaacg ccatgattga catgtaagca tgttagtttt ctataactat ctttataatc
     1741 atttagtagt tgatacaata aacacatact atcaatacct gtagaaacag cgacaacaat
     1801 atggtcatca acatgccaac cattactatt taactgcata acatcacctc acttttacaa
     1861 ttttcaaaaa tggtataagt cttagttata tcattacaaa taaaagagac tgctccttat
     1921 ttaagaaaca gtcttagatt gtctaacgct gcaatcgatt attcgatacg catcattcta
     1981 aatatattcc ttatcgaagc agtctttatt tattttaatt aacgtcttga acctttgcca
     2041 ccgcgtctag attctgtttg acgtttgatt gaagttaatt tatcttcact atcttttaag
     2101 aaattgctta atttcttttc aaaatcttcg gctttttgca ctggtttttg atgacttggt
     2161 ttactcgtat gttgtctacg tggacggtct ttagctttct taattgaaag actaattttt
     2221 ccatcatcag caatagataa tacttttacg tctacttcat caccaacaga aaggtgctct
     2281 tctacgtttt caacataatt atctgcgact tcactaatgt gaactaaacc actttttcct
     2341 tcaggtaatt ctacgaatgc accaaacttt ttaataccag tgactttacc tttaagctta
     2401 tttccaactt cgattgacat atgttaaata aatcctcccg atttgattcg attttccctt
     2461 attatatcct tgtttggatt ttttcacaat gtctatactc ggataatttt gaatcaattt
     2521 ggatttattt tttcgaagat tttgagctag acgaatcttt gtcttctggc aacctaaaaa
     2581 tcacttcacc tttgttgctt aagtaataat catcacgcgc aattttttca atgtaatctt
     2641 tgtcattcag attattcaac ttttctttta acgcaatttc ttcattttgt tgcttttgaa
     2701 actgtgcttc tttcgctttt cgctcctgtg catcaatatc attgcgatgt ttttggacaa
     2761 caagcaagat tgataaaaca acaattatcg caagtaatac gcccgcaaat actgtaatac
     2821 gcctacgaac aacacgcatt ttcatttttt gacgttgttt tttcttgttc tcttgcgacg
     2881 tgtactgatt ttctatatgt tctactttat ttttcattgc ttgtcacctc caatttacgc
     2941 ttcttcaagt cgacgctctt caatgatttc atacataccc ttcgcgttat ctttagatgc
     3001 atgttcattt aatgcagtta ctttaactgt tactaatttt tgaccaaagc gaatcgtcag
     3061 cacatcttca actttaacat ccgatccagc tttagcaaca ttaccattta ttgtaattct
     3121 accttgatca cttacttctt ttgctagcgt acgtcgcttt attaaccgtg atacttttaa
     3181 atatttatct aatctcatgt attactctcc cctttttcta gccactgctt taacgcggcc
     3241 tcatcaaatg acttatcctt cgtcttctca tataaattta aaaaatgctc tgcaaatact
     3301 ttttcaaata taactcttgg ctgtttacct tcagcatctt tcgccttaga ccaaatttct
     3361 tttaagtcat cgatagtttc agcattggca tcaccaaata tgtgtgggtg tctacgaatc
     3421 attttagcat taagacttgt aatcacttct ttaatgtcga tatacccttc ttttttacca
     3481 atactagtat gcaataacac ttgtaataaa atatctccta gttcttcaat catatgccaa
     3541 tcatcttcat tgtcaatagc ttcgaacaat tcaaatgttt cttcaagtaa ataacgcttt
     3601 agcgtttcat gcgtttgcac tttatcccat ggacaacctt tatcttcatc aactaaagta
     3661 tcaatcactt ccgttgcaaa atcaaagtca tgatacaaat atgtcgatgt tatgattttt
     3721 ggtacgaata cactcgtcaa attattgaat gcattttcat catgatccaa ttcatataat
     3781 gggcatgtca caacgttatc cgcaccatcg cttcgtgcac cagtgacaat ttgaacaggg
     3841 taatcatcag gatatcgttc cattaaagtg attttcaaat tagcagcaac cattgcacta
     3901 taaacttgcg taatcaatgt atgcgttcta acattaagtg ttacttcttg taatgatgtc
     3961 gcatctaaca gtgtgaagcc atcatttgga tctacattaa ctgcttcaaa cacatcatca
     4021 ataaagcttt tcccacctaa aactttcaca tctatatcag tattgtcctt tgctaaagcc
     4081 agtaatttca ctgtagttgt ctcagcaact ctaggatgac ccggaaccgc atagacaata
     4141 tctttttcat tagcagcttc aaccaattgc gccacaatat caatatagac atcttcaaat
     4201 tggttatgtg cttcataaac atggtcaaaa ctctgaaatg ttaattcatc ttgcaatgat
     4261 tctataactg gatgatctaa cgttcttgca taaactttat cttgtgtctt taaaaattta
     4321 tatatcccta gcggcaaatc atcaatgcca tagtttccta agccaacaat cgtaatggta
     4381 tgtgccatca acgtcttcct ttcttaattt gatacaactt tgaaccaaat ggtaaataag
     4441 ttaactcttt atatgtcaaa acattaaatc taaaaatata gaaaaacaat gcaatgatac
     4501 ctaatactgc tgcacataat aattcaatga gtccagtgat tctaccgtgt gttgtcacta
     4561 tgtttaacac gcattgaaca acaatcgaca taaataccat acctaaaaca acattgataa
     4621 aaaatcgtct catcgcatat aagtggtatt ttctcgtgac agcaatatgg ataatcgtac
     4681 cgaatataat taatgataca acagtactaa tgctcgcacc aataatgcca cttaaacgaa
     4741 tcaaaatgat attaagtata aatttaataa ccaatcctgc cgtcatacca acaaaaatag
     4801 gtctcacagc atgttgcgct tgtaataatg ccatatccat cataattaac gatacacaaa
     4861 tgaccgtaat catataaaca cttaacgtta aggttaaatc attcgtctta aaaaacacac
     4921 cgttcattaa aggcaataaa ttaattaatc ctattcctgc tgctgtactt attaaaatcg
     4981 taatctttaa tgacgcattt gcataacgat tcataagtac ctgattattc attttaattg
     5041 cgtcacttaa cagaggaatg agcgcaaaac taaatgttgt agttacaatc aatcccatct
     5101 gaataaatga tgcaccacgg tcatagactc ctttttcagt tatggcaaca tcgaatggca
     5161 cgcgtatcgc ttgaagtgac ttaataatag taacactatc aatcacttgc cataaaatta
     5221 cgattaattg actgatagcg aaaatcaaaa ccgaaagtgc gaactgtttc caaacgatct
     5281 ttgcagtgtt atttaccatt ttaaacttaa aaggtcggtg cgctactaaa taaattgaag
     5341 aacctaaaaa acctatcgtt gatgctaaaa tagcaattgt tcccgcttca tatatcgtcc
     5401 aacctctgtc tacaaaaata acaatagtaa caatgataat acctactcgt ataacttgtt
     5461 ctataacctg ggaaatagcc ggaactgtca tattatttgc agattgataa taacctctta
     5521 acacgccaag catacctata aatataaagc ttaaacttgc agcttgaatc attggtgtta
     5581 aatggccatc acccatcata tgtgcaatat tgttcgcaaa cacaaaaata gcaataaata
     5641 ataatatacc aactaattgt atataagcga ctgcttttgc atatgcttcg tcactatgat
     5701 acttccctat attttgtgta attgcactag gaatggcatt catcgataat atcattccta
     5761 atgctacaat tggatacact tgttgatatg catacaaacc tgtatcgcct aatatatttt
     5821 gatatggaat tcgatataca gcactcagaa ttttaatgac aattaatgca gcagttaaca
     5881 cgacaacgcc attaaatgct tctttatgct tcatcactga ttctcatact ttcttcaatg
     5941 cacttaacta aaaacttcaa actatcaagc cattgatttt gtttcgttaa agtaattgtc
     6001 attgcattat tttgaacacc aaccttcatt gttctaccta aaggttgtgt tgctttgaac
     6061 agcacttcgc catcaatatt ttcagtggct tttacagata aatgaatatc aattattttc
     6121 cctttatctt taatcaacgt gatacctgaa tgtaatgcgt gtacttttat ttccactata
     6181 tcaagcaaac gtgctacttc aacaggataa tcattgaaac gatcaattaa ttcatcttta
     6241 atgtcgataa tttgatcaaa tgtttcagtt tttcgtagct ttttataaat ttcaatttta
     6301 gcttgttcat ttgcaatata ttctgttggc aaatatgcat ccaagtttaa atcaacttcg
     6361 acttctggca cctcagattc tggttcctta ataccacgtt tttcatttac agcttcttct
     6421 aacatttgac tgtacaaatc aaatccaact gtatcaataa agccgtgctg ttgtttacct
     6481 aacaaattac cagcaccacg aatgttcaaa tcacgcatcg caatcttaaa tcctgagcct
     6541 aactccgtaa attctttaat cgcttgtaat cgatcttctg cagtctcagt tagtacctta
     6601 tttgctggat gtaagaagta tgcataacca atacgacttg aacgaccaac acgacctctt
     6661 aattgataca actgactcaa tccaaagcga tctgcatctt caatgatcaa agtatttgca
     6721 tttgggacat cgacacctgt ttcaataatc gtcgtcgtta ctaaaatatc atattcatta
     6781 ttgataaaac ttaacatcgt ttcttctaaa tcgcgctctg tcatttgtcc atgagcaact
     6841 gcaatgttag catctggcat taacatctgg agttgttctc gtttttcata aatggattgc
     6901 actttattat aaagataaaa cacttggcca tcacgggata gttctctttc taaagcttct
     6961 ttgataaaac tcatgttctg ttctaataca tatgtttgaa ctgggaaacg attttctggc
     7021 ggcgtttcaa tcactgacaa atcccgcaca cctagcatac tcatatgcaa tgttctaggt
     7081 attggggttg cagtcaatgt tagtacatct acattatgtt ttaatgtttt aatacgctct
     7141 ttatggcgta caccaaatcg ttgttcttca tctacaatca acagccctaa atctttatac
     7201 tgtatatctt tactaagtaa tttgtgtgta ccaacaacta tgtcaacaaa tccagtttta
     7261 agtccttcct tagtttgttt tatctcttta ggcgttctaa aacgactcat taattgaatt
     7321 tcaacaggaa aatcttgcat acgctcaatt aacgtctcat aatgttgctg agctaaaata
     7381 gttgtaggaa ctaaaaatgc aacctgcttt ccttccatta cagctttgaa tgctgctctc
     7441 actgcaactt cagttttacc ataaccaaca tcaccacata gcaagcgatc cattggacgc
     7501 gatttttgca tgtcatcttt aatttcatcg atagatttag cttggtcagg cgtaagttca
     7561 tatggaaaat ctaattcaaa tgttgtttgc tcagctgtgt cttccccata ttgataacct
     7621 tctgccattt ctctttcttt atataaatca atcaactctt cagcaatatc ttcaacactt
     7681 tgttgaactt tagcttttgt ttttttccat tcactgccac cgagtttatt taattttggc
     7741 gtcttatctt ccgaagctac atatttttga acttgatcca tttgatctac tggaacaaat
     7801 agttgatccg tacctttata ttgcaattta atataatcac gatgcgtttg ccccacttcg
     7861 agcgtctcaa cacctaaata tctaccaaca ccatgatgca catgaacaat ataatctccc
     7921 acatttaaat cttggtaaga cttaattttt tcagcatttg agatagcttt cgtacgtttt
     7981 cgttgctttt tctgttttga tttaaaaagc tcacgctcag taatgacaac taatcccata
     8041 tcaggtagtt caaatccttc agataaactg ccttcaataa tcactgcttg ccccgatgac
     8101 attgagcgat gcaattttgt tattgatgga atatgcattt cacttaacat cgcttgcata
     8161 cgttcaactt tagtttcggt ttcgaccaaa accacgatat gatagttttg attaacatat
     8221 cgttgaaatt cagaacgcat aatgtcatat tgcccataaa attgttggac aggtttacat
     8281 gaaaatttaa taatatgatt tagttttatc ggcattgttg tagcgaataa tgaaaaataa
     8341 gtgacaggat agccttcaat caatgtttca aaatcatcat attttataaa actttgtcct
     8401 ataaatccat taccactttc aataatattg ctaataaacg aatcagactc tactgttaaa
     8461 ctttcttcag tttctttaat acgattaaat tcatcaactg caatgattgc atctttttgg
     8521 aaatactcaa taattgtcga aggtgtttca tacataaacg ctactaagcg acgtagtatt
     8581 tgatgatcaa agtatgtact ttcgaataat ttaaagcttt catacgtttc tttcaaatca
     8641 ttgcgcactg atttatctat tttgggtctt gtattttcat atgcagtttt taactcttct
     8701 ttaagatggc tgatcacttc ttcagtaatg atataatcac ttgcagttgt gatatcgact
     8761 tcttcaacat tatctttgga acgctgcgtt tcaacatcaa aatcccgaat agaatcaatt
     8821 tcggtatcaa atagctcaat tctgattggt tccccaatta gcggaaagat atcgataata
     8881 cctcctcgca atgagaattc accaatatgc gataccacgg attcccgttt gtaccccata
     8941 ttaactaatt tgttaagaaa ttggtccaca tcgatatcct caccaacacg caatgtcatt
     9001 tggtgatttt gccacatttc aacaggagtt aaccactttt tcaaaccatt taaaggaacg
     9061 ataaataacc ctttcttacc ttgagctaac gcagttaaag ttctaatacg ttcactcatc
     9121 agttgagggc tttgtgttga aaactcttcg gtcataatat cttgcacagg atacttatac
     9181 aattcttcag catctataaa ttgaagtaaa tctgtttcta atttatctgc ttggtataaa
     9241 ttattggtaa ttaataataa ctgttgatta ctttgtgcat atttttcagc aatcatcgtc
     9301 actttagctg acggggaaag accagttact agtgtgtttg cttgtccaaa tacctgatta
     9361 aggtcttgaa aatgattatc ttcttttata agcgttgtca atattgtcat tatttcactt
     9421 caccattaaa ttcattcata acatggtcaa atcgtgatgt ttcaacaaac ttttcaattg
     9481 cgcgtgctgc gtgttcgata actttttcca tcgttaccat ttcatcattt gaaaagcgtt
     9541 gtaaaacata atcaggtacc gtcataccat tcgttggtct tcccacacca atacgaatac
     9601 gtttaaattg gtctgtacca agcattttaa taattgattt cataccattg tgaccgcccg
     9661 cacttccttt ttgtcttaag cgaacttgtc cttgttctaa atctaaatca tcatataaga
     9721 caattaaatc ttctggatta acattgtaat aatccataat cggtgcaact gcttcacctg
     9781 acaaattcat cattgtcatt ggttcgataa ataacacttt atcgccgttc attcgttcaa
     9841 ttgtatatgc acctttaaac ttttgtttat ctaatgaaaa attatttttc tctaaaatat
     9901 aatcaacgac ttcaaagccg atattatgtc ttgtaagttc aaaacgttta cctatattac
     9961 ctagacctac aatacatttc atgcgtgttt acctccaaca taattgtttc atttattatt
    10021 ataatgacaa catccatttt ccgccattgc catcaattat cattattctt tagcttagta
    10081 agtatgtatt ttaaaatcga gtacattaaa atttttatga tgtatcattg ttgagtttaa
    10141 tgtcttttac tcactaaaat taattaaact ttagtacaaa gtaataaagt ttaattaagc
    10201 tttataataa cacaaaaagc accatgctta tattaaaata agcacagtgc ttgttaaaca
    10261 aagtataaaa actttaatgt aacagattaa aattactctt cagttttttc ttcgtcttct
    10321 ttgctttcgc caacaacttc tggttcttca gtttgttgtt cgccttccat agcttcgatt
    10381 tcttcttcag ttggttcttc agttggagca actactgtta ctactgattc agctgaatcg
    10441 ttttcgattt tgaagtcgcc agttacttta acatcagcaa cagttaagct gtcgttaatg
    10501 tttaattcag taatgtctac ttcgattgct tctggaatat tgtctggagt agcagttact
    10561 tctaagttga ataatggttg ttcaactacg ccgccttctt tagcgcctac tgcttcacca
    10621 actaattgaa ctggtacttc aacagtacgt tcttcactca tattgattgc taagaagtca
    10681 atgtgagtaa tttggttttt aagtggatcg aattggtagt ctgcaaccat aactttgata
    10741 gttttagaac caacgcctaa ttcgataaca ccgttacgac ctacttcacg gataactttg
    10801 atgaattcta cttcatcaac tttaactgac acgtttttag taccgtaacc gtatactact
    10861 gctggtactt taccagattt tcttaattgt ttaagatctg aacgtgtttg tttaccttga
    10921 cggatgattg actttaatga agccattctc atttccacct ttcatttttg ctcaaaacga
    10981 gcacctcact tacccatcaa catcatcgtt gcttgcaagt gtttatttat tcgtataatc
    11041 acgaacgaaa ctattataca cgtttggaat atgttcgtca aacgcctttt aaatattagt
    11101 caaataatac gctaactgat tctctttcgt atacacgaat gatagcttgt gcgattaaac
    11161 cagcaacaga taattcttta gtgttagatg gtttgcgatc ttcatctaaa tgaattgagt
    11221 ttgttacgat taattctttt atagcagaat tttcgatacg ttctttagcc ggtcctgata
    11281 aaacagggtg tgtacaacaa gcatatactt ctttagcacc tttatctttt aatgcttgtg
    11341 cagctaaagt gattgtacct gctgtatcaa taatatcgtc aataataatt gccgtacgtc
    11401 cttcaatctc accaacaatg ttcatcactt cagcaacatt tggtctagga cgacgtttat
    11461 ctataattgc aattggagtt tttaaaatgt cagctaattt acgtgcacgt gtaacgccgc
    11521 catggtctgg tgaaacaacg acacattctt ctgggttaat attcggatca tctttgaaat
    11581 gtttagcaag aattggcaca cccattaagt ggtcaattgg aatatcaaag aatccttgaa
    11641 tttgtggtgc atgtaagtct aacgcaatca tacgagttgc gccagctgtt tcgattaagt
    11701 ttgcaactaa tttagcagtg attggctcac ggctacgggc ttttctatct tgtcttgcat
    11761 atccataata tggcactaca atattgattg ttgctgcaga agcacgttta caagcatcaa
    11821 tcataattaa taattccatt aaatgtagat tcacaggata tgatgttggt tgaataataa
    11881 atacgtcaca accacgaata ctctcttcga tattaatttg aatttctcca tcactaaaac
    11941 gtttaactga acatttacct agttcaattc ctacttggtc agcaacttct tgcgctaatg
    12001 cttcgtttcc tttcaatgaa aaaatcttta atgacgaatt cttatattca ttatttaaca
    12061 tttatagtcc tccaattatt tacttacgca attactatta tatataaaat gtaactccat
    12121 tactatgcga atcattaaat cataaagatt acattaatat tacataggat tatcctttta
    12181 tcctagccat tttaaatacg taaatgatta tttcctatat ccttcttttg ttgtttgtct
    12241 tgctcttgcc acagctaaac tgtcatttgg tacgtcatct gtgattgtgg aaccagctgc
    12301 caccaataca tcatcaccaa ttgttacagg tgctactaaa ttaacattgc aacctacaaa
    12361 tgaatctttg ccgacgatag ttttaaattt attttcacca tcatagttaa ctgtaatcgt
    12421 tccgcaacca atattagtac gttcgccaat tacagcatcg ccaatataac ttaaatgtga
    12481 aaccttggca ccatctttaa gatctgcttt tttaatttct acaaaatttc caaccttaac
    12541 atctgcacct aattgcgcgc ctggtctcaa ttgcgcaaac ggtccgacct tagtattcgc
    12601 tcctacgcta gcatcattaa caacagactg ttgaatacat gcaccatttt caatcgtact
    12661 attgttaatt tcagagtact gaccaataac aacatcttcg ccaatttctg tacgaccatt
    12721 aattcgtacg cctggttcaa tgactgtatc actaccaatt gtaacgtctg gaccaatata
    12781 agtgctgtca ggatcgatga ttgtcacacc atttagcatg tgataatgat tcgtacgacg
    12841 ttgcatcgcc ttctcagcct gactaagcat tacacgatca tttacaccca tgatttcttc
    12901 aacatcattg gtacgataga cttctacgat gccgccatca tttaaaatta acgacaatac
    12961 atcagggagg taatattcac cttgcgcatt atcatttttc acttgtgtta atttttcaaa
    13021 caacgtttta ttattaaacg caaaaatacc tgaactaatt tcattaatat ccttttcagc
    13081 ttgcgttgca tctttctctt caactatgcg ttctaaacga cctgacgcat ttcgaacgat
    13141 tcttccgtat ccatatggtt gttgaatcga tgcagataat acagttgctt gagcattagc
    13201 atcctcgtga tgcgcaatca atgtttctaa tgtttccttt gtgatgagcg gtgtgtcacc
    13261 acatacaacg attgtcgtac cttccttgtc ttctaagtgt gatttcgcca tttgcactgc
    13321 atgcgcagta ccgagttgtt cctcttgaaa actgtataaa gaacgctcgc ctaaatgtcc
    13381 ttttacactt tcagcaccat gtcctacgat ggttacaact tgatcgacac cagagccttt
    13441 cacactttcc aatacatgtt cgaccatagg tttcccagca acctcgtgta gcactttata
    13501 ctttttagat ttcattcttg tgcctttacc tgctgccaaa attatcgcgt gtcttcgcat
    13561 gaacattaac ccccattaaa ttttacactt cgtcattatt ataatttaat ctgcaatact
    13621 ctttcaacat ataaaaggca ttgtttttaa ttcgttgtaa ttttaaaaac aacttttttc
    13681 ttacttcaaa aatacgagca tcattcaaac gcaattttta tcagcaatga atcaataatt
    13741 ttcatatcat cataatctgt taacgtatat atttattagc ctttcattaa aaataaaaaa
    13801 acattaatac aaactaaaaa aactgccgac aaagctttat gctttatcga cagtatcaaa
    13861 tcaacttatt gcaaatgtat tacatcgcta aatataaaat tgattaagct tcttctgaat
    13921 cttctgatgt agcgttttta tctggtacta cttcatctgt ttcatcatat actttcatca
    13981 ctgcatcttg aatttcttgt ctcatatctg aattaatagg atgcgcgatg tcgcggaatt
    14041 caccatctgg tgtacgttta cttggcattg caacgaacaa gccagagttt ccttcaatta
    14101 cacgtaaatc atgaattacg aaagcttcat ctaatgtaat ggaaacgagt gctttcattc
    14161 gtccatctgt ttgtattttt ctaagtctta catctgtcac tttcatgtag tgagcccccc
    14221 tatagtatat atctctctgt tttatttaga agcttgatta aacatattaa tcgaaaatta
    14281 taattcctta attttactta ctaattcaat ttctactttc acatcttttg gcaaacgcgc
    14341 aacctctaca caactacgcg ctggcttgtg ttcattaaaa tattgaccat acacttcatt
    14401 tattttttgg aaatcattca tatctttaat gaaaatggtc gctttcgcaa cagaattcaa
    14461 atcagatcct gcttcttcca aaacaacctt taaattttct aaaacttgtt ttgtctgtgc
    14521 ttgaacatca gcgcttacga tatgtccatc aatattcaat ggaatctgac cagaagtata
    14581 aaccatacca ttcacaactg ttgcatgcga atatggtcca agtgcttccg gtaatcttgt
    14641 tgtgttaatg attttcataa tactaaaact ccttttatga aaacttagat aaactgttgc
    14701 caggttctac gttaaactct tgattatatt catctacatc agataatttc actaaggaag
    14761 tataatcttc aatcaatctt tgtttaactt cttttgattc tacaagtact gatacccctt
    14821 ttacatgggc tttaaactca ttcattaaat tcataacacc attaatagag ccaccagccc
    14881 tcataaaatc atcgacaact aaaacatttg aattttctgc taaagttctc ttcgaaagta
    14941 ccattgtttc tatttttctt gaagatcctg aaacgtaatt aattgaaact gtagaacctt
    15001 cagtcacctt gttgtctttt ctaatcacta ctactggtaa atttaaaata ttagcaaccg
    15061 catttgccaa tgaaatacct tttgtcgcaa tggtaacaac agcatctaat ttttcttcca
    15121 tgtaaatact ggcaattaac ttaccaactt tgtttagtag cgatggatta cctaccaaat
    15181 ctgataaaaa taaatatccg ccaggtaaca aacgttcttt ctcttctaat agagtaatga
    15241 cctcattaac aacttcagtc gcctcttctt tactcatcat tggtttatac gtaacaccac
    15301 cacttgcgcc agcagtagta attactgtac ctaacttttc tttttggaat gtatttttta
    15361 taatttggac atcttcactt attgaagact tcgcctgttt aaattttttc acaaaaaaag
    15421 ttaatggaat caatttattc ggatggttca tcaaatattg cgtcataaaa acaattctct
    15481 cgcttcgttt atatctcatc ttttcaaccc ttctatccta atagtctaac taagtacact
    15541 tcattacaac aaccgttaac tgcattataa atattttttg cttggctttc ttttcgtgct
    15601 agcccataca cagtaggtcc gcttccactc attaacgcac catctgcacc acttttcaac
    15661 atattatttt ttaatttatc gatttgtggg tgttttgaaa cagaaattgg ctctaatcga
    15721 ttagacaaac tttgacataa ttgttgataa tctcgatttt ctaaggcctc ataacacatt
    15781 ttcgtatgta cgtcgtaacg cttatctaaa ttaatcaact taaatatatc tggtgatgat
    15841 atgcctaagt ttggtttagc aagaatcacc caagctgaag gtggtttatt taaaaactcg
    15901 attttctctc ctcttccagt acatagtgca gttttattat aaatacaaaa cggaatatct
    15961 gtcccgattt tactgcctag tagagccaat tcttccaaac tcgcccctat atcaaaaagt
    16021 cgattcaatc ctcttaacgt tgctgctgca tcagccgaac ctccagctaa gccagcagaa
    16081 acaggtattt ctttatcgat agaaattgtt acaccttgct ttagttgata ttgctcaata
    16141 aatagttgcg ctgcacgata tgcgagattt ttatgattag aaggcacata attatgttca
    16201 atctcaacaa ctatctttcg atcttttctt ttatgaaaag ttaaacgatc atttaaatca
    16261 actgttgtca ttatcatttc aatctcatga tagccatcat ttcttttaaa aagtgtatcg
    16321 agcgtaaaat taattttggc tggtgccgtt tcatatatca ttttccgccc tcttttcgtt
    16381 atcttaatat gatgatttta acataaagag aattaatatt tgacatttta atttgttttt
    16441 tagtgttttt ttaataaaaa agtaattttc tccatctaat tacttagtat ttacttgtat
    16501 ttgtgtttat aaatacaatt atccatttac tgaaaaatct ataagcgaaa catcagtgtt
    16561 aaacattttg taaataaaaa gagtattgaa cataatgccc aacactcaaa taattatcat
    16621 aatatttact tcttatacta agaaacgtct aacatctcta tatgtcttat ttagtgtgca
    16681 attgattcgt gatgattatc ctcttcaaat gaaacttgaa cattttcagt taacacatca
    16741 gtgtatgtat aagatactct ctcaaagttg tgtttgtctt gatctaactc aacaatgaaa
    16801 actgacggat atgtttcttt taaaattcca gaacgtttta ttgttttctt acggcctcca
    16861 ttggctttca gtacaatacg atttcctaca tgacaatcaa tagaattttt gatgtccaaa
    16921 attgattttg gcatattgct ccacctcgct acaagttaca taataccata tttgcaacgt
    16981 tttcgtcaaa caaaatttaa attttaacaa tagtgctttg ctttgtcaat catttaattt
    17041 tctaattgag ggaatttttt cttttcttca tacaatttag caaaatcttg aatagatagc
    17101 gtttcaccgc gacgtcttgg atcaatacct gcttgttcca accattgtaa aatcacttct
    17161 ttgtgttgtt taccatcttt aaaataattt tgatagttat tgttaattgt ctttcttctt
    17221 tgtgcaaatg ctgcttttgc taacttaaag aatgcttcct cgttatctac tgttactaac
    17281 ggttcagttc tctgcatcag ttttacaact attgaatcaa cattaggtgg tggcataaat
    17341 acagatttag gtaccgttaa tactttacta gtctctgtat agtattgtac gacaattgat
    17401 aacgaaccat atgcttttga acctacttca gcatttaagc gttcgcccac ttctttttgc
    17461 atcatcacca cgtagccatc aattggtata tcttgttgca tcaaatttaa taaaattggc
    17521 gtcgtaatat agtacggcag gtttgcaaca accattattt tttcacaatc ttgtaaatga
    17581 ttttcaacag cttctttaat attcgctttt aaaatatctt cattaatcac cgtcacatta
    17641 tcataaggtg atagtgtatc atttaataca ggtattaaac gttgatcaat ttcaaatgcc
    17701 aatactcttt tagcatgtct ggccaattgt tctgtcaatg accccatgcc tggaccaatt
    17761 tcaatcaccc cagtttgtgc atcaatatca cttgcatcaa tgatattatt aatgatattc
    17821 acatctatca aaaagttctg tcctaaactt tttttaaaat taaagccata tttatctaac
    17881 aacgctcgcg ttcttgatgg tgttgcaata tctttattat ccaacatttt cacttcctca
    17941 ttcatcttct aaagcttgcc ttacatccgc ttcggtataa ccaaatgcat ttaacttttt
    18001 caataactgc ttaccattgg aatggccgat tcgcaatttt ctacttaaaa tttctctacg
    18061 gcgccttgca tctttcccaa caattaaccc caactctatt agcacagatt tatcaattga
    18121 ttcataagct tcatcaaagg gtgaactaac atgcattaac gcttctttaa tatcaattaa
    18181 ttcggcatgt tcaacaccaa ttttcccttt tttattttta gctttttctc tatcaatata
    18241 cgcatgttta acacctttga catgttcagt aattgtactt ctaattttat ctcctgggaa
    18301 atctggatct gttaatacaa taacgcctcg actttgttga gcatttctaa ttacttctaa
    18361 agtttgttcg ttgatggcac taccattcgt ttcaatcgta tcacattcaa cagctcgttt
    18421 aacacgctca gtatcatctc gtccttctac aactataaac tcattgattt tcatttaaaa
    18481 cgccttccta caattcttaa tttcaaagct ctaaccatct ctatcgcacg tattccacat
    18541 gcacgatatt ttaatttaaa aaagtgagaa agaaccatag catattaaac gaatatagca
    18601 tcgtttacat ttatggcggt gatctttctc acttttaact ttatgaattt aaattaaaca
    18661 atttctctgc atttttagtt gtttgttcgc acacttcttc ataagataag ccttttaatt
    18721 cagcaatttg ttcagctact aaagttactc tcgccggttc atttcgcttc cctctatacg
    18781 gatgtggcga aagatacggt gcatcggttt caactagcaa acgctccatt gacacatgct
    18841 tagcaacttc tttaggctgt ttagcatttt taaatgtcac aggtccacct aatgaaatat
    18901 aaaaattcag cttattagtt acaatatctg caatttctgg agaaccacta aagctatgca
    18961 taatcccgcc tacctcttca gcatgctcct ccaataagat atcgatacag tcttgagttg
    19021 cttcacggtt atgaatgata attggtaact tcaaacgctt agctaaagca atttgctttc
    19081 taaaaacttc cttttgaaca tctgcaggag atttatccca gtgataatct aatcccattt
    19141 caccaatacc aatcactttt ggatgctgag ctaaagattc aatccattcc aagtgttctt
    19201 ctgtaaaatc aattgcgtca actggatgcc aaccgataat gccatataaa aaatcatact
    19261 catcgattaa tttcatcgcg cgttcaattg tcgatttgtt aaaaccaact acaaacatac
    19321 gatcaacacc tgcttctcta gcacgtgtaa tcacttcact caaatcatca tcgtattgct
    19381 catcatttaa atggacatgt gtatcgatta acataattat ctctcctaat ttttaaaaat
    19441 acagttattt aatcactgca ccatttggaa ttgcacttgg taaacttact aaggttaata
    19501 caccatcttt ttcagcagat aatatcatac cttcagattt ttgtcccatt aatttagctg
    19561 gtttcaggtt agtaacaact gctacttttt taccaataat atcatctggt gtatagaatt
    19621 tggcaattcc tgatacaatt tgtctttgtt cagaatctaa gtctacttga atttttaaaa
    19681 gcttatctga cttcttaaca tgttcagcat caataatcgt tgctgcctta atttcaactt
    19741 tatcaaagtc tttaatatca atttgaggtt tgctaggaat ctcttctttt tcctctttag
    19801 tagcaggcgg ttgcattgat tctttgatat atgcaatttc cgcttcgcta tccaatcttg
    19861 ggaaaatagg tttaggttgc ccagtaacca taattgactc attaagcaca ccatattgct
    19921 ctaaactact aaattccata aattgaggat tgttaatgtt caattgttca aaaatctctt
    19981 tcggcgcatg tgttaagaat ggacgtaata atacagctgc ataacgaata ttttcaacta
    20041 agtgagccat tacattgcct aacatatctt tttggctatc gtccttagct aatacccaag
    20101 gcgttgtttc gtcaatatac ttattcgttc tactaataaa cttccatacc gtagataatg
    20161 ccacagaaaa ttgcaaactt tccatgcttt cagtgtagct tttcactgtt tctaaagcca
    20221 tagcttccat ttcttcatct aattcatgaa gtggaccttg atacgctggt aattcgccat
    20281 caaagtactt attaaccata gaaatcgtac ggtttactaa gttacctaag tcatttgcta
    20341 gatcgaaatt tgtacgctca acaaatgctt caggtgtaaa tacgccatct gaaccaaatg
    20401 gtaattcacg cattagataa taacgtgtag catctaaacc atagcgatca attaaaatat
    20461 tagggtctac gacattacct ttagatttac tcatttttcc atctttcatc aaaatccaac
    20521 catgtgcaaa gactttttta ggtaacggta agtctaatgc catcaataaa ataggccaaa
    20581 taattgagtg gaatcgcaca atttccttag ccattaaatg aatatctgct ggccagtatt
    20641 tgttaaatag tgactcatca tctgataaat agcctaatgc tgaaatatag ttaactaacg
    20701 catcaatcca aacataaaca acatgttttg gattagacgg aacatggaca ccccagttaa
    20761 atgatgtacg agaaacagct aaatcagcaa gtcctggttt aatgaagttg ttaatcattt
    20821 cattttttct tgatggtggt tgtataaaat ctggattttg gtcatagaac tctaataaac
    20881 ggtctgtata tttactaata ttaaagaaat aactttcttc tttaactagt tcaacttcgt
    20941 gtccagaatc tggacttttg ccaccaataa ttttaccgtt ttcgtattgt gggtctacta
    21001 attgtgactc tgtatagtat gtttcatccg gaacagaata ccaaccttca tattcaccta
    21061 aatagatatc accttgcttt aataaacgtt caaacacttg ctcaacgaca tgtttatgac
    21121 gttcttcagt tgttctgata aaatcatcat ttgaaatttc aagcttagcc cacaattgtt
    21181 taattccagc aatcatctca tccaaatatt caatttctgt cttaccagat ttttgagctt
    21241 tttcttgaat tttttgaccg tgttcatccg ttccagtcaa atagcgaaca tcatatcctt
    21301 gcattctctt atatcttgca ataacatctc cagccactgt agaatatgca tgtcctatat
    21361 gtaaattccc actaggatag tatattgggg ttgttatata aaatgtttct ttagccataa
    21421 ctgttcctcc tcgtaatgtc tcacttcaaa taatatctaa gatttctatg catttcaacg
    21481 gacgcgaaca tttatctcgt catctcattt tcatcttatt caatgttaag gtatttatta
    21541 ctttcaatgt atttatttta cattaaaaaa cgtcgatgtc atcatttttt tatcatagtc
    21601 aacatcattt tatcagtatc aatgcaaaat gataacttac catttaaaat tacagcttgc
    21661 gtttataaaa tcgtttaaaa tttcatataa tcgataaagt gattaactta tttgatgata
    21721 aatattatat acttcattcg ttttaagttg tcgttcttca gcaacttttt taatagcttg
    21781 ttttggtttc atctgtgaag tttgaatata atgatcaaca tgctcattga tagataaatc
    21841 atcaaaccac gatatctcat tgttcgcttt agcaccttca attaagataa cgaattcgcc
    21901 tttcaatggt acatcgcctt gctgaatcaa tgcttgtaat tgtgttacat catcagttac
    21961 aatttgttcg aacttcttag ttaattcacg ccctagtgat acttgtcgtg ttgcatctat
    22021 ctttgcaatt gtttttaatg tatctgtcac acgatgcggt gattcgtata taattaatgt
    22081 gctattttca tgcatacgtt gctctaatac agcacttttt tctttctctt ttcgtggcaa
    22141 aaatcctaaa aatgtatata catatgaagg taatccacta gccatcaaag ccgtcagccc
    22201 agcattaggt ccaggcacgg tctctacttt aatattagct tctctggctg ccactactaa
    22261 ttcgtatcca ggatcactaa ttaagggcaa tccagcatca gatacgagcg caacgtcaag
    22321 acctaattct aactgttcaa tgataaaagc agtctgctta tccttgttat gttcgtgata
    22381 tgactttaat ggagttggaa tatcataatg attacacagt ttactagtta ctctagtgtc
    22441 ttcacaagca atcatatcaa cacgtttcaa tacatcaact gctctataag taatatctgc
    22501 taaattacca attggtgtgc ccactaaata taatacagcc atactatcgc tccttaatta
    22561 atcccaattt cttttgtctg gtataagttt taatttcata ttcacgcttc aatgcttcag
    22621 actttgtctc atacatttct tgataaacta aatgcaccgg acgtcttact ttcgtatatt
    22681 tggctccttg acctcggtta tgtttttcaa cacgtgcatt aacgtcttta gcgtatcctg
    22741 tatataaact tccatcacta cattttacaa tatatacaaa atgactatcc ataatatact
    22801 tccttcattt cttcgctata attaccatct tcattataaa tataaaatgg gggcatgatt
    22861 tctaaacctt gatttccacc ttttcgacct tctactacta tcgtttgtgc tgatttccct
    22921 actttactat atataaaaac gactttctta ggttcaatat tcacttttct catttcaaac
    22981 aagacatcca ttagtctctc tgcacgatgt accatgttta gcctgccacc ttcttttaat
    23041 aaatgacggg ctgcaatcat gcaatcttca agtgtacaca taatctcatg tctcgctatc
    23101 ttatgtgctt ctttttgatg ttggtgttgc tgattctctt taaaataagg cggattacac
    23161 gttactaaag tatattgtga aggtttaaat actttagtaa cgtttttcaa atccatgtga
    23221 tgcattgtta aatattcatc aacatcattg aattgaaatg tgcgtcgcgc catatcgaca
    23281 agtgtttttt gaatctcaac accttctata tgtcgtggat gtttcgcaaa caataacaag
    23341 ggtatcaccc cattgcctga acataagtcc aacacaatat cttttgttct aggttttgta
    23401 aaatgcccta acaacaaagc atccgttgaa aatgaaaaaa catcatcatt ttgaataata
    23461 ctaaaatctt ctttgattag ttgatcaaat cgttcattct cttttaacat ttttaactcc
    23521 tatgaagaca acctttttct attgagtaat tgaaaactct ttttatatta tatgcctaac
    23581 tacattcgat gtatttttga cttttaacag ctacatttta taactaatga tactgcgtgc
    23641 acacttaata ataacattta gtaccgccat ctcttatgca atcaccaaaa ctttatacac
    23701 aattaaagcc tgaaacatgc attgtctcag acttatccaa accatattca tttataatta
    23761 taacactatt tgagtgtgct tgattaatca cttaaaactt ctaaacagaa cagacaatct
    23821 tcaccatgtc gatgttttcc aaataattcg cctttacaaa tatgaaatcc ttctccatac
    23881 aatatagcca aattatcttt acttggtaat ggctttttca cagcttttgc tggttttgaa
    23941 ttcgcagtat caatagtagt tggttcatca ttgcccaaca cctttttcaa attatcattt
    24001 tcaagttgaa gcgctacatt ttcttctact aattcaactg caagtgcctt taattctgaa
    24061 gtttcctttg aaagttgatt gacattcatt tctaaacgca ttattttttc aaatatttca
    24121 ttgcgatcca aatgtaataa tgcctcctta atgcatagtt tctatttctt ctaatttata
    24181 ttcaagtggc tgttcatgtc cctcaagctt cacctgcata gaaatgtcta atatatttaa
    24241 agcaactact ttcccgttac catcaggcgt ttcaattgct tcaccaatat caggtaattg
    24301 tgcacgtact tcctcataat agtcattttc atattttaaa caacacatca aacgaccaca
    24361 tgcaccagaa attttagttg gatttaatga taaattttga tccttagcca tcttaatcga
    24421 tactggttca aaatccccta aaaatgtaga acaacataac gacctaccac aaggtccgat
    24481 accgccaagc aatttggctt catcccttac accaatttgt ctcaactcga tacgtgtttt
    24541 taaatgttgc gctaatattt ttactaattt tctaaaatca atacgatcat ccgccgtaaa
    24601 attaaaaata actttcgatt tatctaatgt atattcgcaa ttgactaaac gcatgtccaa
    24661 accttgttct cttacaatgt ctttacatag tattaatgca ttttcagcat ctcgttcatt
    24721 acaattaaat ttatcaatat ctttgtcatc agcaatgcga ataatatttt taagaggtaa
    24781 cacaacatcc tcttcagcaa tatccattaa tggattttta acaataccta tctctatgcc
    24841 tcttttagat tcgacaacta cccagtcttc tatatctact tgtatatcat taggtgtata
    24901 atattctaat tttcccgctt tttgaaactg aacacctatt acatttggca tctaactcac
    24961 acccttaatt acgatttgtt caaatacaag cgttggattt acattttgat tcaatttctt
    25021 atgtgcttcc gtcagttgat caaacatcaa aattaattga ttaaacgaca acttttgcgc
    25081 atattgatca atatcatttt ttaaatcact atatatttgt ttatcctcta catttacctt
    25141 tgtatgtatg atatcttcga agaaaccatt cacagctgca atagttaaag attgcagttt
    25201 tttattttta gcctgtttca ataaatcaat aatacctata agtgccattg gcttattagt
    25261 aagcaacaat tcacaccaac gtataactga tttccttaat gctaataaat caaattcttc
    25321 atttaaagcc attgcattat ctatttgcgt agtataagta ctaatcattt cagctactgg
    25381 cttagacatg ttttgttcaa ctaatctatt tataaacttt tctttatcaa taggcttgaa
    25441 atatacatgc tgacatcttg aatggattgt gtctaaaatt tgctcaggtt ttgtagacaa
    25501 taaaatagca atcgtattgt ccggtggttc ttcaagaaat ttcaagatac tgttttcccc
    25561 ttgaacagtt aacttttcaa agtcttcaat gatgtacact ttatttgtgc tttctatagg
    25621 aagttgattc atatgacgca caagttgttc aacttgttct ttcttaattg cattctcagt
    25681 tgttgatata tacataaagt ctggatgatt atatgtacta acctttgttt cacattgact
    25741 atctgtttga cataaaataa gctttgcaaa attaatcgca acttgtttca tcgtttgtgc
    25801 atcatcacct tcaaataaat aggcatgcga taatttattt gaatgatatg cattcgtcaa
    25861 ttgttgctgt tcatccattc attacaaact cccttttgct tttatataaa aaggttgcca
    25921 aagagcaact aattactgca acaatagcga ccatgatttg tgattatttt tagaaccaca
    25981 aaaatcaaat ctattggcat ctgaactagt cgcagtcaag caacccaaaa ttgttaataa
    26041 cttattaata cactatttta agtatctcta attgatgatt gtattataga attaaaattg
    26101 atggaatgca tcaactggca taacaaatac agtagcaccg ccaacttcaa cttcaactgg
    26161 atatggaatg tacgaatccg cactgcctcc cataggtgta ataggtgaaa ccaactgttc
    26221 tctattacca cacgtttgat taatcacaga caatatttca tctacacggt catcattgac
    26281 accacataag aatgttgtat tacccgctct taaaaaccca cctgttgttg ccaattttgt
    26341 tgctctaaag ttatttttaa caagttgatc tgcaagttcc tgactatctt gatcttgtac
    26401 gatcgctata atcattttca ttttataaca cctcttctaa caattatatc atatcttttc
    26461 taaatatttg atgatagttt gatacgtgtc ttcaacaaca ttttcaagag gttgatctgc
    26521 attaacgctt ttgaaccgtt gtgattcatt atgaatgatt tcttggtaac cttcaattac
    26581 tttttcgtga aactttaaat cttcttgatc taatctattt tgatctcttg aatttttaat
    26641 aatacgttcg cgacctactt cagcactaac atttaaataa atcgtcaagt ctggatataa
    26701 tccatttatt gcaaattcgt ttaatgctct tacttcttca acgccaatcc ctctagcata
    26761 accttgataa gctaatgaac tatcgatata gcgatcacac aacacaacct taccttcttt
    26821 taaagctggt atgaccttta atacaagatg ttctcttcta gatgcagcaa ataacattgc
    26881 ttcagttcta atgtccatat cattgccttc taatacaatt ttacgtattt cttcaccagt
    26941 aggaacacca cctggttctc tagtcataat gacatcataa tcttttacta atctatggta
    27001 aacttcatta attacagttg tttttccaga gccttctggg ccctcaaaag ttataaaagc
    27061 tgacatttta ttcatcctca actaaaattt tattattttt aattccttca actatcattc
    27121 cagtttccag atattcattt accaattcta tcatattttc agttattgtt tctcctttga
    27181 aaataatagg aatccctggc ggatacggga caatatgtcg cgccagaact ttaccttttg
    27241 cctttttcaa atcacaccaa gtaacatatt caaagcgttt tggtttatag ttaccttcag
    27301 ttgttaaaag ttgtgtttgt ttaactttag aaactgattt tttcggtaaa atcatatctt
    27361 caattttacg taaaagcgaa tcaaataaat acgtatcatc atgatgccat aacggcaata
    27421 tcgctaatgc ttgatagtcg tccgctaatt ctaaatagat atgtgcattc ataaaccaat
    27481 tttgaatatc atgacctgta aaaccttcat attttatcag caacttcaac ggatcatcaa
    27541 cttgaagcat ttcaaaaccc ttcttctcca aacattcgat taattgcgct ctcttatcaa
    27601 aaaacacggt actatcatat gttttataga actcggcagc tgactctaaa ctagccataa
    27661 tcaaatacga aggactagat gtttggaagt agcttagata ttctataata gtttctctat
    27721 aaggtgcatt tttatgaata taaagtaccg agcccatcgt taaagctggt aacgttttat
    27781 gaaaagattg aacaacatag tcagcttgat aatttaatgt agaatctgga aatccttgca
    27841 atccaaagtg cgcgccgtgt gcttcgtcaa tgagtacagg aatatttaat tggtgcaaaa
    27901 atttgataac ctcttctaca ttaaatgttt caccgtaata gttaggataa gtcaacacag
    27961 caagtttgtg accgtcatta ttcaaacggc ttaaattaac tttattataa tgattcgtta
    28021 acggactttg atgcgtttca ataaaatgcc cttcttgttg gctaatatcg agcgcatgta
    28081 acacagattt atgtacattt cttgccatta agatatcgcc ttttttctgt gaaaaagact
    28141 ggatgacaga taatattcct gaagtggtgc cattcactaa gaaataagca tcataatctg
    28201 aatgtttctc cacctgcttc atactttcca aaatgacttc ttcaggatga tgtaaatcat
    28261 ctaatccagg tatttcagtt ttatccattg tcattgataa ttgagataaa tgaccgatag
    28321 tcatattttt atgacccgga acatgcaaag aaatcgcttc ttcttgattt aaactttcta
    28381 atttatttaa aataggttgc ttcatgatat acgcttcctt tatttacact gttttggaat
    28441 tagttacttt caaaagtatt aattatatag taacacttct ttgacaaaag ttagtgttac
    28501 ttatgcaata gcttgtctat tgtataataa ttaatttcct ttttgtattt caataaagta
    28561 tataaaagag cagtaagatc attttcaatt agataccgtc ttactgctct tctctattta
    28621 tacaatattt tgttttcgat gatatcattt taaatttata taaacacaaa attaaagttg
    28681 tacatactta gacaatcgaa agtgtacatt attaaattat catttccagt tctactctag
    28741 cggaacgtaa gttggctacc atcctcgcta agaacctttc ttgacttgtg acaatcgctt
    28801 gcttctttcc tctccttcgg ctctcactta ctcatttagc tctactaaac tcgttgcgtt
    28861 cttttctcgt ttcgtcagat tcaaacgttt tcacttcgcc aagccatttt tctttgtgtt
    28921 tgctttttat tttgacgttt tagacataaa aaaaagagac cttgcggtct caatgcggct
    28981 catcgcatcc att
//
LOCUS       DAKTIO010000015        26729 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.015, whole genome shotgun sequence.
ACCESSION   DAKTIO010000015 DAKTIO010000000
VERSION     DAKTIO010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 26729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26729
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.015"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            116..1501
                     /gene="fumC"
                     /locus_tag="O0293_000507"
     CDS             116..1501
                     /gene="fumC"
                     /locus_tag="O0293_000507"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCY0279417.1"
                     /translation="MSVRIEHDTFGEIEVPADKYWGAQTERSKRNFPVGKERMPIEVV
                     YGFAQLKRAAAIANFDLGKLSEAKKDAIVYACDQILSGELDEHFPLVVWQTGSGTQSN
                     MNVNEVVSYVANMYLKDHQSDESIHPNDDVNKSQSSNDTFPTAMHVALYQEVETKLEP
                     ALKLLRNTLKEKEDKFDSIIKIGRTHLQDATPIKLGQEISGWRYMLDRCETMLSESKK
                     HILNLAIGGTAVGTGINAHPEFGDKVAHYISENTGYPFVSSENKFHALTAHDEVVQLH
                     GTLNALAGDLMKIANDVRWLASGPRAGLAEISIPENEPGSSIMPGKVNPTQCEMLTMV
                     AVQVMGNDTVVGFASSQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPI
                     EENIDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQFEA
                     WIKPEDMVDPH"
     gene            complement(1964..2785)
                     /locus_tag="O0293_000508"
     CDS             complement(1964..2785)
                     /locus_tag="O0293_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCY0279418.1"
                     /translation="MKFKIPENFNDLSLRDIFQQLKVPKKDLHHLNMSKDITINDKPA
                     RLMDKVHTGDDVFVPTIDEKSNYVPSYRYAQIKYEDDDMAIVMKPKGVKTHPNDLKES
                     NTLMNHVIYTIDSDYVEPIHRLDQETVGLLIVAKNPLMKKILDRMLEDNDITRIYKAN
                     VKALLPLKPQTIDMPIGKDKFHSNKRRVSPTGQRAITHILTSKMIKEAVCQLEIKLDT
                     GRTHQIRVHLAEIGHPVIGDPLYGDSTLRQLELESYKIEFVHPLTKEVISVSLDD"
     gene            2948..4060
                     /locus_tag="O0293_000509"
     CDS             2948..4060
                     /locus_tag="O0293_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HCY0279419.1"
                     /translation="MEQRTRLALLKEIAEFLNEETEMYSMTQGALKYLIEGSNFTTGW
                     IFFINSVGEHELVSHVALPQSLTADHCHYIKDGSCWCVKAFNQRRLMKASNIVNCSRI
                     NLASKAFPSQNDNITHHATVPLKSGQEQFGILNVASPNTEIYSDEDLELLESVAFQLG
                     SAIKRIYLTDREKEAAKINERNRLARDLHDSVNQMLFSVKLTAHAAYGMSNESIAKQA
                     FKTIEETSQNAVNEMRALIWQLKPVGLEQGLIHALTAYSKLMHIQLNVNVEGLIDLSN
                     EIEENIYRALQECINNVKKHADTNKMDLTLKQMNDILYIDVIDYGQGFEIDNVQIASS
                     HGINNIKQRVKLLRGKVTFHSQPTKGTQIQFTIPIK"
     gene            4082..4705
                     /locus_tag="O0293_000510"
     CDS             4082..4705
                     /locus_tag="O0293_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0279420.1"
                     /translation="MNKVILVDDHYIVRQGLRFLLSTIENIEVLQDFADGETFLEYLK
                     EHEHPDIVLLDLVMPGMNGIEITEYIKAHYPDIKVLVLTSYVDDEHVISAINKGADGY
                     EMKDVEPQQLIETIRRVMNGEKMIHPKAQDVFETVSQKPHYTNKLSKREIEVLREMVK
                     GKTNKEIAETLFVSEKTIKTHVSHIFSKLQVSDRTQAAIYAMENKLI"
     gene            5061..5525
                     /locus_tag="O0293_000511"
     CDS             5061..5525
                     /locus_tag="O0293_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0279421.1"
                     /translation="MDRQSFTDLIQTKFKMVRIEAGYTQDTMAQTIGLSKKTLVQIEK
                     ERVLPNWTTCISICALFRDSEVLNSTFGCDPLEIVQTISRNHCAYPNHAPTSDIYWNN
                     IETRNGYILQSNKVSNIYRVLNPDNQPIFGTSKMREAETYFNRNAKEELVHI"
     gene            5704..6828
                     /locus_tag="O0293_000512"
     CDS             5704..6828
                     /locus_tag="O0293_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HCY0279422.1"
                     /translation="MNALFIIIFMIVVGAIIGGITNVIAIRMLFHPFKPYYIFKFRVP
                     FTPGLIPKRREEIATKIGQVIEEHLLTETLINEKLKSEQSQQAIESMIQQQLQKLTKD
                     QLSIKQITSQIDIDLEQVLQTNGNQYIESQLNNYYTKHQNQTIASLLPNQLVTFLNQH
                     VDNATDLLCDRARNYLSSAKGTQDINDMLDTFFNEKGKLIGMLQMFMTKESIADRIQQ
                     ELIRLTSHPKARTIVTSLITNEYQTFKDKPLNELLDASQFNEIAENLSVYVTTYASKQ
                     ANKPVVTLMPQFVDYLEGQLSSKLANLIIEKLSIHLSTIMKKVDLRGLIEEQINTFDL
                     DYIEKLIIEIANKELKLIMSLGFILGGIIGFFQGLVAIFV"
     gene            6897..7241
                     /locus_tag="O0293_000513"
     CDS             6897..7241
                     /locus_tag="O0293_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HCY0279423.1"
                     /translation="MAVNLYDYANQLEQALRESEEYKAIKEAFANVKANEESKKLFDE
                     FRETQINFQQKQMQGEEIAEEDLQKAQEQAQAIEKDENISALMNAEQKMSQVFQEINQ
                     IIVKPLDEIYAD"
     gene            7624..7722
                     /locus_tag="O0293_000514"
     CDS             7624..7722
                     /locus_tag="O0293_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279424.1"
                     /translation="MPFDSLAVLITTEIAFPTEIFEKYQRTLQIKF"
     gene            8013..9209
                     /locus_tag="O0293_000515"
     CDS             8013..9209
                     /locus_tag="O0293_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCY0279425.1"
                     /translation="MVKFIHCSDLHLDSPFKSKSHISPKIFEDVQKSAYESFKNIVDI
                     ALQQDVDFVIIAGDLFDSENRTLRAEIFLKQQFERLQNEQIFVYVCHGNHDPLSSKIS
                     SNWPDNVSVFSNKVETYEAITKSGETIYIHGFSYENRASYENKIDEYPSSQGQKGIHI
                     GVLHGTYSKSSVNERYTEFILEDLNSKLYHYWALGHIHERQQLSDMPVINYSGNIQGR
                     HFNEQGEKGCLLIEGDHLKLKTKFYPTQYIRFEEATIETDKTSKQGLYEVIQNFKEQV
                     REEGKAFYRLTLVINSETLISPQDLLQVEEMITDYEENENQFVYIDELKIQYAQNDES
                     PLVNEFSAELLVDQTVFDKAMSDLYLNPRASKFLDDYGTFDHTALVNRAEEILKAEMR
                     GEQNDN"
     gene            9199..12135
                     /locus_tag="O0293_000516"
     CDS             9199..12135
                     /locus_tag="O0293_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCY0279426.1"
                     /translation="MIIKSLEIYGYGQFVQRKIEFNKNFTEIFGENEAGKSTIQAFIH
                     SILFGFPTKKSKEPRLEPRLGNQYGGKLVLILDDGLEIEVERIKGSAQGDVKVYLPNG
                     AVRDDAWLQKKLNYISKKTYQGIFSFDVLGLQDIHRNLNEKQLQDYLLQAGALGSTEF
                     TSMREVINRKKDELYKKSGKNPIINQQIEQLKQLESQIREEEAKLETYHRLVDDRDKS
                     SRRLENLKHNLNQLSKMHEEKQKEVALHDHSQEWKSLEQQLNIEPITFPEKGVDRYEK
                     ARAHKQSLERDIGLRNERLAQLKEEATQLEPVKQSDIDAFISLNQQENEIKNKEFELT
                     AIEKDIANKQRDKDELQSNIGWSETHHDVDSSEAMKSYVSEQIKNKQEQAAYIKQLER
                     SLEENKIEDNAVHSELDSVEEKIVPEETFEKKKEYSQQVIELNEKENLYSKLKERFEI
                     EQQEKQKRQKLLRTTFILLTLVGIGLTAFSFISNNMLFGIIFAVLTLVFVIGIIMSKS
                     KEVDYSEAITDEIEEIKAQLAILDENYDLDFDLDEQYRIRDHWQQALKNKDILEEKRQ
                     YIEGRLNDAKGRHDELQSTVENVKDELYLSSKISNDLIVDSISTMANIKALDQHISDL
                     NQQRQQLVQELDTFYNHAEAVTKSQFVYFNKLSLFHDVQQWLKSAEDTNEKWRINAEN
                     TKLVTNELNHLNAQLEENNKEITALFDFINVGTEEDFYQHHEDYQTYTSNLSRFNDLT
                     KYLENQNYSYELSSSLSEKTTAQLEEEDHLLATQVDEYNEQYLEMQAQVSDLSAQINH
                     METDTTLANLRHEYHSLKNQLNDIAKDWASLSYLQSLVDEHIKQIKDKRLPQVINEAV
                     EILKHLTDGRYTMINYNEDSITVKHVNGQLYDPVELSQSTKELLYVALRISLIKVLRP
                     YYPFPLIVDDAFVHFDKKRTEKMLNYLRSLSEHYQVLYFTCVKDNIVPSKEVITLNKI
                     EEGGKR"
     gene            12132..13073
                     /gene="yhaM"
                     /locus_tag="O0293_000517"
     CDS             12132..13073
                     /gene="yhaM"
                     /locus_tag="O0293_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM"
                     /protein_id="HCY0279427.1"
                     /translation="MRNIENLNPGDSVDHFFLVHKATQGVTAQGKDYMTLHLQDKSGE
                     IEAKFWTATKNDMATIKPEEIVHVKGDIINYRGNKQMKVNQIRLATTEDQLKTEQFVD
                     GAPLSPAEIQEEISHYLLDIENANLQRITRHLLKKYQERFYTYPAASSHHHNFASGLS
                     YHVLTMLRIAKSICDIYPLLNKSLLYSGIILHDIGKVRELSGPVATSYTVEGNLLGHI
                     SIASDEVVEAARELNIEGEEIMLLRHMILSHHGKLEYGSPKLPYLKEAEILCYIDNID
                     ARMNMFEKAYKKTDKGQFTDKIFGLENRRFYNPESLD"
     gene            complement(13194..14156)
                     /locus_tag="O0293_000518"
     CDS             complement(13194..14156)
                     /locus_tag="O0293_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HCY0279428.1"
                     /translation="MKMINKLIVPVTASALLLGACGASATDSKENTLISSKAGDVTVA
                     DTMKKIGKDQIANASFTEMLNKILADKYKNKVNDKKIDEQIEKMQKQYGGKDKFEKAL
                     QQQGLTADKYKENLRTAAYHKELLSDKIKISDSEIKEDSKKASHILIKVKSKKSDKEG
                     LDDKEAKQKAEEIQKEVSKDPSKFGEIAKKESMDTGSAKKDGELGYVLKGQTDKDFEK
                     ALFKLKDGEVSEVVKSSFGYHIIKADKPTDFNSEKQSLKEKLVDQKVQKNPKLLTDAY
                     KDLLKEYDVDFKDRDIKSVVEDKILNPEKLKQGGAQGGQSGMSQ"
     gene            complement(14361..14918)
                     /locus_tag="O0293_000519"
     CDS             complement(14361..14918)
                     /locus_tag="O0293_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HCY0279429.1"
                     /translation="MFLCKRQIDINARFGLPRIAFMSAVATIIMFLVSYEVMYFLSNT
                     PLSDRHFLIFLLLVFMTYPLHKSIHLLFFLPYRKSFKVHKLTKRKWLIFYNTYVNQPV
                     HKFYFCINLILPLIILSAMFVYLTISFPQYGHYFMFLLALNFGISITDLLYLKIIIFS
                     NYGQYIEEHSTGINILKKIKNPYHL"
     gene            complement(15651..16016)
                     /locus_tag="O0293_000520"
     CDS             complement(15651..16016)
                     /locus_tag="O0293_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCY0279430.1"
                     /translation="MKASRILFGIGVGVAAGFVVALQGRDDKSVKNNTIDRTAPTGSK
                     SELQREFETIKQSFNDILNYGVQIKNESAEFGSSIGGEIKSLLGNFKSDINPNIERLQ
                     SHIENLQNRGEDIGNEISK"
     gene            complement(16158..16580)
                     /locus_tag="O0293_000521"
     CDS             complement(16158..16580)
                     /locus_tag="O0293_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HCY0279431.1"
                     /translation="MSETIFGKILTGEIPSFKVYEDDYVYAFLDISQVTKGHTLLIPK
                     KASANIFETDEETMKHIGAALPKVANAIKRAFNPDGLNIIQNNGEFADQSVFHIHFHL
                     IPRYENDIDGFGYKWETHEDILDNDAKQQIAEQIQAQF"
     gene            16714..17454
                     /locus_tag="O0293_000522"
     CDS             16714..17454
                     /locus_tag="O0293_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0279432.1"
                     /translation="MTVKVEQLTGGYGKRPVIKDINFELNKGEIVGLIGLNGAGKSTT
                     IKHMLGLLTPMEGSLSISDININDDIEAYRRKLSYIPESPVIYEELTLEEHIEMTAMA
                     YDIDRDETMNRAMPLLKTFRLENELKVFPSHFSKGMKQKVMIICAFIVNPELYIIDEP
                     FLGLDPLGIQSMLDLMVEKKNEGRTVLMSTHILATAERYCDRFIILDEGEVVAFGDLE
                     ALRQQTGLHNQTLDDIYIHVTQGGDVHA"
     gene            17447..18670
                     /locus_tag="O0293_000523"
     CDS             17447..18670
                     /locus_tag="O0293_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0279433.1"
                     /translation="MRNQATTLFNKRLHALRKEKNYYNKFIFNGHFMVFLLILLGAFI
                     FGYGEWLKHIPTNINFSLIAAVIVALTSIFPMRPLLKEADKIFLLPFEKHMSQFMRHA
                     ILYSYFARILIQLIIVIVMFPLFYNINQHNVAFYICFGVSALIFPYVGLRLRWQWYQS
                     GLKTWQVNLISFITFALTYYLLLAPKWYIAFVMVALPVLIEFLVKKYKPGFLYPWEKM
                     IAIEHRHHMNYYKFVNMFTDVKHLKESAVRRSYLDILLPVPKGSKFNSNAMYLFLFIR
                     SFIRGRDAFNIIFRLVIIAVLLMVWLSYPLVTAIIGCLFVYIILLQMAQFYSQQAYGL
                     WPQVWPVPEEKVIKGYEQFLYRLMFVICTVFAVTFIIKHMTLFYVVLIFYIVGLLTIR
                     SIIKKLKYQETLLRD"
     gene            complement(18794..19297)
                     /gene="traP"
                     /locus_tag="O0293_000524"
     CDS             complement(18794..19297)
                     /gene="traP"
                     /locus_tag="O0293_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein TRAP"
                     /protein_id="HCY0279434.1"
                     /translation="MKKLYTSYGTYGFLHQIKINNPTHQLFQFSASDTSVIFEETDGE
                     TVLKSPSIYEVIKEIGEFSEHHFYCAIFIPSTEDHAYQLEKKLISVDDNFRNFGGFKS
                     YRLLRPAKGTTYKIYFGFADRHAYEDFKQSDAFNDHFSKDALSHYFGSSGQHSSYFER
                     YLYPIKE"
     gene            19460..19570
                     /locus_tag="O0293_000525"
     CDS             19460..19570
                     /locus_tag="O0293_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279435.1"
                     /translation="MIKCQFYDIYLLKIRTNERYDNIDKNDFNLGGLYGA"
     gene            19560..20597
                     /gene="hemE"
                     /locus_tag="O0293_000526"
     CDS             19560..20597
                     /gene="hemE"
                     /locus_tag="O0293_000526"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCY0279436.1"
                     /translation="MVHNKNNTILKMIKGEETSHTPVWFMRQAGRSQPEYRKLKEKYS
                     LFDITHQPELCAYVTHLPVDNYHTDAAILYKDIMTPLKPIGVDVEIKSGIGPVIHNPI
                     KTIQDVEKLSQIDPERDVPYVLDTIKLLTEEKLNVPLIGFTGAPFTLASYMIEGGPSK
                     NYNFTKAMMYRDEATWFALMNHLVDVSVKYVTAQVEAGAELIQIFDSWVGALNVEDYR
                     RYIKPHMIRLISEVKEKHDVPVILFGVGASHLINEWNDLPIDVLGLDWRTSINQAQQL
                     GVTKTLQGNLDPSILLAPWNVIEERLKPILDQGMENGKHIFNLGHGVFPEVQPETLRK
                     VSEFVHTYTQR"
     gene            20655..21578
                     /gene="hemH"
                     /locus_tag="O0293_000527"
     CDS             20655..21578
                     /gene="hemH"
                     /locus_tag="O0293_000527"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCY0279437.1"
                     /translation="MTKKMGLLVMAYGTPYKESDIEPYYTDIRHGKRPSEEELQDLKD
                     RYEFIGGLSPLAGTTDDQADALVSALNKAYADVEFKLYLGLKHISPFIEDAVEQMHND
                     GITEAITVVLAPHYSSFSVGSYDKRADEEAAKYGIQLTHVKHYYEQPKFIEYWTNKVN
                     ETLAQIPEEEHKDTVLVVSAHSLPKGLIEKNNDPYPQELEHTALLIKEQSNIEHIAIG
                     WQSEGNTGTPWLGPDVQDLTRDLYEKHQYKNFIYTPVGFVCEHLEVLYDNDYECKVVC
                     DDIGANYYRPKMPNTHPLFIGAIVDEIKSIF"
     gene            21602..23002
                     /locus_tag="O0293_000528"
     CDS             21602..23002
                     /locus_tag="O0293_000528"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HCY0279438.1"
                     /translation="MTKSVAIIGAGITGLSSAYFLKQQDPNIDVTIFEASNRPGGKIQ
                     SYRKDGYMIELGPESYLGRKTIMTELAKDIGLEQDIVTNTTGQSYIFAKNKLYPIPGG
                     SIMGIPTDIKPFVTTKLISPLGKLRAGFDLLKKPTQMQDGDISVGAFFRARLGNEVLE
                     NLIEPLMGGIYGTDIDKLSLMSTFPNFKEKEEAFGSLIKGMKDEKNKRLKQRQLYPGA
                     PKGQFKQFKHGLSSFIEALEQDVKNKGVTIRYNTSVDDIITSQKQYKIVYNDQLEEVY
                     DGVLVTTPHQVFLNWFGQDPAFDYFKTMDSTTVATVVLAFDEKDIENTHDGTGFVIAR
                     TSDTDITACTWTSKKWPFTTPEGKVLIRAYVGKPGDTVVDDHTDNELVSIVRRDLSQM
                     MTFKGDPEFTIVNRLPKSMPQYHVGHIQQIRQIQAHIKQTYPRLRVTGASFEAVGLPD
                     CITQGKVAAEEVIAEL"
     gene            23325..23879
                     /locus_tag="O0293_000529"
     CDS             23325..23879
                     /locus_tag="O0293_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase family protein"
                     /protein_id="HCY0279439.1"
                     /translation="MTDVIIVHSMHGNSRNHWYQWLEHNLTLEGYDVTLFNFESPEAK
                     TVDQWIEAMTKQINVRKKDTYFVTHGLGSITALKYIEMIDQPIEGFFSIAGFKEDAEN
                     IDLDIDLSNVTIDYDNIKKKVDNFYGLSSKNDKYVSYKETQRLMNALEGNLRIVEDGG
                     HFLEEDGFETFTALQDRMQDYMTR"
     gene            24351..24424
                     /locus_tag="O0293_000530"
     tRNA            24351..24424
                     /locus_tag="O0293_000530"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24385..24387,aa:Met,seq:cat)
     gene            24446..24521
                     /locus_tag="O0293_000531"
     tRNA            24446..24521
                     /locus_tag="O0293_000531"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24479..24481,aa:Asp,seq:gtc)
     gene            24538..24610
                     /locus_tag="O0293_000532"
     tRNA            24538..24610
                     /locus_tag="O0293_000532"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24571..24573,aa:Phe,seq:gaa)
     gene            24624..24699
                     /locus_tag="O0293_000533"
     tRNA            24624..24699
                     /locus_tag="O0293_000533"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24657..24659,aa:His,seq:gtg)
     gene            24715..24788
                     /locus_tag="O0293_000534"
     tRNA            24715..24788
                     /locus_tag="O0293_000534"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24747..24749,aa:Gly,seq:tcc)
     gene            24803..24877
                     /locus_tag="O0293_000535"
     tRNA            24803..24877
                     /locus_tag="O0293_000535"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24835..24837,aa:Asn,seq:gtt)
     gene            24879..24950
                     /locus_tag="O0293_000536"
     tRNA            24879..24950
                     /locus_tag="O0293_000536"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24912..24914,aa:Glu,seq:ttc)
     gene            25005..25093
                     /locus_tag="O0293_000537"
     tRNA            25005..25093
                     /locus_tag="O0293_000537"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25040..25042,aa:Ser,seq:gga)
     gene            complement(25667..25875)
                     /locus_tag="O0293_000538"
                     /pseudo
     CDS             complement(25667..25875)
                     /locus_tag="O0293_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26336..26729)
                     /locus_tag="O0293_000539"
                     /pseudo
     CDS             complement(26336..26729)
                     /locus_tag="O0293_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500456.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1829 domain-containing protein"
BASE COUNT         9512 a         3823 c         4671 g         8723 t
ORIGIN      
        1 tgcgattagt gtatttttat ttcaaattat gaaaacgttt taatcataaa ttgctcaatt
       61 aaattattta atgatatact acagttaaat aaacagtgat aaggggagaa attgaatgtc
      121 agtaagaatt gaacatgata cttttggaga aatagaagta cctgcagata aatattgggg
      181 tgctcaaaca gaaagaagta aacgtaattt cccagttggt aaagagcgta tgccaatcga
      241 agtagtttat ggttttgcac aactaaagcg tgcagcagca atagctaatt ttgatttagg
      301 aaaattaagc gaggcaaaga aagatgccat tgtatacgca tgtgatcaaa ttttatcagg
      361 tgaattagat gaacacttcc cactagttgt atggcaaaca ggaagcggta cacaaagtaa
      421 tatgaatgtg aacgaagtag taagttatgt tgctaatatg tatttaaaag atcatcaaag
      481 tgatgaaagt atccacccaa atgatgatgt aaataaatct caaagttcga atgatacatt
      541 cccaactgct atgcacgttg cattatatca agaggttgaa acaaaattag aacctgcatt
      601 aaaactttta agaaatactt tgaaagaaaa agaagataaa tttgattcaa ttattaaaat
      661 tggtcgtaca catttacaag atgcaacgcc gatcaaacta ggacaagaga ttagtggctg
      721 gcgttatatg cttgaccgtt gtgaaacaat gttatctgaa tctaagaagc acattttaaa
      781 tcttgccatc ggtggtacgg ctgttggtac tggtattaat gcgcatcctg aatttggtga
      841 taaagtggca cattatattt cagaaaatac gggttatcca tttgtatctt ctgaaaataa
      901 attccacgca cttacagcgc atgatgaagt tgttcaattg catggaacat tgaatgcatt
      961 agcaggagac ttaatgaaaa ttgctaatga tgtgagatgg ttggcttcag ggccacgagc
     1021 tggtttggca gaaatttcta tccctgaaaa tgaaccaggt tcatcaatta tgcctggtaa
     1081 agttaatcct acacaatgtg aaatgttaac aatggttgca gtccaagtaa tgggtaatga
     1141 tacagttgtt ggcttcgcaa gttcacaagg taactttgaa ttgaatgttt ataaaccagt
     1201 tattatgcat aatacactac aatcaattta tcttttagct gatggtatgg aaacatttaa
     1261 taacaattgt gcagtgggca ttgaaccaat cgaagagaat attgataatt atttaaatca
     1321 atcattaatg ttagttactg cattaaatcc acatattggt tatgaaaaag cagctcaaat
     1381 tgctaagaaa gcccataaag aaggtttaac tttaaaagaa tctgcaattc aaactggata
     1441 tgttacagaa gaacaatttg aagcatggat taaaccagaa gatatggtag atcctcatta
     1501 attaaatatt aatataaaat aaagtttggg cattacaggt agatgtagtg ttcaagcttt
     1561 attttttgtc tatgtcaaat tgaacttata agtctgattt gatatagagc catgaaaagg
     1621 aaaaactttt catggagtcc gttttattat agaacctatt ttttatatga tgaattgttg
     1681 tacatgtatt ggccaaacaa cctgtgataa aaatcttatg atgtcatgta ttgtccatag
     1741 atacacgtaa cgcagttgaa aaagactaca agtcattatc caaggatgtt ataaaatgaa
     1801 taattactaa aatatcaaaa tataaaatcc taagcatcgt aaaaaacaaa aaaaccgaac
     1861 atctgaaaaa attaataatt ttccatctat ccgatttaag ttgatacttg atttagttac
     1921 aatgcggtat tgcatgttta aatatcatgc aaaactaatc aaattagtca tccaaagaaa
     1981 cggaaatgac ttccttagtc aagggatgca caaactctat tttgtaactt tcaagttcta
     2041 attgtcttaa cgttgaatca ccatataaag gatcaccaat aacagggtga ccaatttcag
     2101 ctaaatgcac acgaatttga tgagtacgtc cagtatccaa cttgatttca agttggcaca
     2161 cagcttcttt tatcattttt gaagttaaaa tgtgtgtaat tgcacgctgt ccagtaggag
     2221 acacacgtcg tttattcgaa tggaatttat ctttaccaat tggcatatca atcgtttgtg
     2281 gttttaaagg taaaagtgcc ttaacatttg ctttgtatat ccgcgtaata tcattgtctt
     2341 ctaacatgcg atcaagaatt tttttcatta aaggattttt agcaacaatt aataatccta
     2401 ctgtttcctg gtccagtcga tgaattggtt cgacatagtc actatcaata gtgtaaatca
     2461 catgattcat taaagtattg ctttctttta aatcattagg gtgagtctta acacctttag
     2521 gtttcattac gattgccata tcatcgtctt cgtatttaat ttgtgcataa cgataacttg
     2581 gaacataatt acttttttca tcgatggttg gaacaaatac atcgtcgcca gtatgcactt
     2641 tatccattaa tcgcgcaggt ttatcattaa tagtaatatc tttagacata tttaaatgat
     2701 gtaaatcttt tttaggtacc ttaagttgtt ggaaaatatc tcgtaaactt aagtcattaa
     2761 agttttctgg tattttaaat ttcataaaat cctcctatag tctttaatat catatcacaa
     2821 atcagttctc cgaaatataa ctattgagta atcaaggcca ttttatcatg tttacgtcta
     2881 agatttaaaa atataataac tcaatgtata atagttaata cacgtaaaaa tgaattggag
     2941 cgatttgatg gaacaaagga cgcgactagc tttattaaaa gagattgccg aatttttaaa
     3001 cgaagaaact gaaatgtata gcatgaccca aggtgcgtta aaatatttaa ttgagggcag
     3061 taatttcacg acaggctgga tatttttcat caatagcgta ggtgaacatg aacttgtatc
     3121 acatgtggct ctaccacaat ctttgacagc agaccactgt cactatatta aagatgggtc
     3181 ttgttggtgt gtgaaagcat ttaatcaacg taggctaatg aaagcgtcga atatcgtcaa
     3241 ttgttctcgt attaacttag cctctaaagc atttcctagc caaaatgaca atattacaca
     3301 tcatgcaacg gtaccactta aatcgggtca agaacaattc ggcattttaa atgtagcgtc
     3361 tccaaatact gaaatatata gcgatgaaga tttagagttg ctagagtctg tcgcatttca
     3421 attagggtca gccatcaaac gtatttattt aacagaccgt gaaaaagaag cggcaaaaat
     3481 taatgaacgt aatcgtttag ctagagattt acatgattca gtaaatcaaa tgttattttc
     3541 tgtaaaacta acagcacatg ctgcgtatgg catgtcaaac gaatccatcg ctaaacaagc
     3601 attcaagacg attgaggaaa cgagtcaaaa tgccgttaat gaaatgcgtg cattgatttg
     3661 gcaacttaag ccagttggac ttgagcaagg tttaattcat gctttgacag catatagtaa
     3721 attaatgcat atacaattaa atgtaaatgt agaaggttta atcgatttat ctaatgaaat
     3781 cgaagaaaac atatacagag cattacaaga gtgtattaat aatgttaaga aacatgctga
     3841 tacgaataaa atggatctta cattaaaaca aatgaacgat atactatata ttgacgtgat
     3901 tgattatggt caaggatttg agatcgataa tgttcagata gcttcatcac acggtattaa
     3961 taacataaaa caaagagtta aattattaag aggtaaagtg acctttcatt ctcagccaac
     4021 aaagggtaca caaatacaat tcacaattcc tataaaatag cttgatttaa ggagataacc
     4081 catgaacaaa gtaatattag tagatgacca ttatattgtg cgacaaggat tgcgattttt
     4141 attatccacg attgaaaaca tagaagtttt acaagacttt gcagatggag aaacattttt
     4201 agaatattta aaagagcatg agcaccctga tattgtgcta ttagatttag tgatgcctgg
     4261 catgaatggt attgaaatta cggaatatat taaggcacat tatccggata ttaaagtttt
     4321 ggtattaaca agttatgttg atgatgaaca tgtaatttca gcaatcaata aaggtgctga
     4381 tggttatgaa atgaaagacg ttgagcctca gcaattaatt gaaactatta gacgagttat
     4441 gaacggtgaa aaaatgatac atcctaaggc acaagatgta ttcgaaacag ttagccaaaa
     4501 accacactac acgaataagt tgtcaaagag agaaattgaa gtgttacgtg aaatggttaa
     4561 aggtaaaaca aataaagaga ttgcagaaac tttatttgta tctgaaaaaa caattaaaac
     4621 acatgtcagt catatattta gtaaattaca agttagcgat cgtacacaag cagcaattta
     4681 tgcaatggaa aataagttga tttagaatga gcttttaaat atttgtcgaa aaatagacaa
     4741 tttttaatct gattttgatg aattttttat agcgataggg tttattatac agttttcttt
     4801 gtaaatttga tatcattata aaagtgtaag ttttatcaaa tctagtgatt atgcataaaa
     4861 atttacttca tttactttcg cttcattata aagtaaactt aactcttgtt gacttgatca
     4921 aaatttaata caaatgtgaa attgactctt ttcattttat taaaaatata ttaagatgta
     4981 atctgtgttt caagaaatgt gtattattgc aatttcttgt gtccggataa aattccccaa
     5041 acagaaaggt agataacaga atggatagac agagttttac agatttaatt caaacaaaat
     5101 ttaaaatggt tcgtatagag gctggttata cgcaagatac tatggcgcaa acaattggac
     5161 tttctaaaaa gactttagta caaatcgaaa aagagagagt attaccaaac tggacaactt
     5221 gtatttcaat ttgtgcatta ttcagagact ctgaagtatt aaacagtaca tttggctgtg
     5281 atccattaga aatcgttcaa acaatttcaa gaaatcactg tgcatatcca aaccatgcac
     5341 caacaagtga tatttattgg aataacattg agactcgtaa tgggtacatt ttacaaagta
     5401 acaaagtaag caatatttat cgtgtattaa atccagataa ccaaccaatt ttcggtactt
     5461 ctaaaatgag agaagctgaa acatacttta acagaaatgc taaagaagaa ttagtacata
     5521 tctaggccta tatactttta ataaataata aaagcgatgt aacacacctg tttcgagagg
     5581 tgaggcatcg ctttttgtat gtaaactaat aataaattga gcagaaagtg aaatgattaa
     5641 tgaattaaaa aataaaaata gtggtataat gaataactat gttgtcacaa aggagtcaaa
     5701 attatgaatg cactatttat tatcatattt atgattgtag taggggcgat aattggtggc
     5761 attactaatg taattgcaat tagaatgctg tttcacccct ttaaaccata ctatatattt
     5821 aaatttagag tcccatttac accaggttta ataccgaaaa gacgcgaaga aattgcaact
     5881 aaaattggcc aagtgattga agagcatttg cttacagaaa ctttaattaa tgaaaaattg
     5941 aaaagcgagc aatcacagca agcaatagaa tctatgattc aacagcagtt acaaaagttg
     6001 acgaaagatc aattgtcaat aaaacaaatt acttctcaaa tcgatattga tttagaacaa
     6061 gtattacaaa ctaatggaaa tcaatatatt gaatcacaat tgaataatta ttatacaaag
     6121 catcaaaacc aaacaatagc atctttattg ccaaatcaac ttgtaacatt tttaaatcag
     6181 catgtagata acgcaacaga cttattatgt gatcgtgcaa ggaattattt atcatctgca
     6241 aaaggcacac aagatattaa tgatatgttg gatacttttt tcaatgagaa aggtaagtta
     6301 attggtatgt tgcaaatgtt tatgacaaaa gagagtattg cagatcgcat tcaacaagaa
     6361 cttatacgtt taacatctca tcctaaagca agaacaattg tgacatcgtt aattaccaat
     6421 gaatatcaaa cttttaaaga taaaccattg aatgaactgt tagatgcatc gcagtttaat
     6481 gaaatcgctg aaaatctatc agtgtatgta acaacatatg caagcaaaca agcgaataag
     6541 ccagtggtta cgttaatgcc tcaattcgtt gattatttag aaggtcaact ctctagcaaa
     6601 ttagcaaatt taatcattga aaaattatcg attcacttat ctacaattat gaaaaaagtg
     6661 gatttacgag gattaattga agaacaaatt aatacgtttg acttagatta tattgaaaaa
     6721 ttaattatag aaattgctaa caaagaacta aagttgatta tgtcattagg tttcatttta
     6781 ggtggtatta ttggattttt ccaaggtttg gttgcaatct ttgtctaaaa aagcgagatg
     6841 tggtaaagta atatcgaacg ttttaataaa catcacaaca aaggagtgct ataaccatgg
     6901 cagtaaattt atatgattat gcaaatcaat tagaacaagc tttaagagaa agcgaagaat
     6961 acaaagcaat caaagaagca ttcgctaatg taaaagctaa cgaagaatct aaaaagttat
     7021 tcgacgagtt ccgtgaaact caaattaact tccaacaaaa acaaatgcaa ggtgaagaaa
     7081 ttgctgaaga agatttacaa aaagcgcaag aacaagcgca agcaattgaa aaagatgaaa
     7141 acatctctgc attaatgaat gctgaacaaa aaatgagtca agtattccaa gaaatcaacc
     7201 aaattatcgt taaaccatta gacgaaattt acgctgacta atattaaaat aatatttttc
     7261 aatcctttaa atagaggaat agactgccga cagagtccga gactttgtgg gtagtttttt
     7321 tagttttgat aacggaagtt agaggctctc tgtcaaattg ggcagatgca ttcagtaacg
     7381 gaagttaggc tagtaaacgt gtcctaactt cctttttgca ttttgaagtg taaagttttc
     7441 aacataatac tattagttcg gtcatgtatc ggactgatgg aaaagcgttt cacttttaat
     7501 gactcattaa gaacggcctg aaaatgtttg gcgtattaag tgcaatgata gttttgacat
     7561 ttagtttcta attggtcatt actgccgagc aaatctagta gagtaatcat gtaaatcttt
     7621 aatgtgccat ttgattcact agcggtgtta ataactacgg aaattgcatt tccgactgaa
     7681 atttttgaaa aatatcaacg tacgctacaa ataaaatttt aaactgttat aaatgtgtct
     7741 caatttcata tgttcatcga cgatatgaag cgtattatgg taaaatgaag aaataataaa
     7801 cttgttaata aataaaacat cacgatttga ctaaagcact ttattattgt gtagataata
     7861 gttttttaac gaaataaaaa tggcgactgg ttttaataaa tcagctaatg aatcactaca
     7921 cctataagta tgaatatagt gattagaatg ctttgtatag ttggattttg caaaattgat
     7981 gttagaacct ttaattaagg agatatggat gtatggttaa atttattcat tgttcggatt
     8041 tacatttaga tagtcctttc aaatctaaga gtcatataag ccctaaaatt tttgaggatg
     8101 ttcaaaaaag tgcttatgaa agttttaaaa atattgtaga tattgcatta caacaagatg
     8161 ttgattttgt aattatcgcg ggtgatttat ttgatagtga aaacagaaca ttacgtgctg
     8221 aaattttctt aaaacagcaa tttgaacgtt tacaaaatga acaaatcttt gtttatgttt
     8281 gtcatggtaa tcacgatcct ttatcatcaa agatttcatc aaactggcca gataatgttt
     8341 ctgtattttc aaataaagtt gagacgtatg aagcaattac taaatctggt gaaacaattt
     8401 atattcacgg atttagttat gaaaatagag caagttatga gaacaagatt gatgaatatc
     8461 catcaagtca aggccaaaaa ggcatacata ttggtgtctt gcatggtacg tatagtaaat
     8521 cttcagttaa cgaaagatat accgagttca ttttagaaga tttaaacagt aaattgtatc
     8581 attattgggc tttaggtcat atacatgaac gtcaacaatt aagtgatatg cctgtaatta
     8641 actattcagg taatattcaa ggtagacatt ttaatgagca aggtgaaaaa ggttgcttat
     8701 taatcgaggg tgaccactta aaattaaaga ctaaatttta tcctacacag tatattagat
     8761 ttgaagaagc aactattgaa acggataaga catctaagca aggtttatac gaggtcattc
     8821 aaaactttaa agaacaagtg agagaagaag gaaaagcctt ttatcgttta acgcttgtta
     8881 ttaatagtga gacattaatt tcacctcaag atttattaca agttgaagaa atgattacag
     8941 attatgaaga aaacgaaaat caatttgtat atattgatga gttaaaaata caatatgcac
     9001 aaaatgatga gtcaccttta gttaatgaat tttcagcgga attattagtc gatcaaactg
     9061 tttttgataa agcgatgtca gatttatatt taaatccaag ggcatctaag ttcctagacg
     9121 attatggaac attcgaccat acagcattag ttaatcgtgc tgaagaaata ttaaaagctg
     9181 aaatgagagg tgaacaaaat gataattaaa tcacttgaaa tttatggtta cggtcaattt
     9241 gttcaacgta aaattgaatt taataaaaac ttcactgaaa tttttggtga aaatgaagcg
     9301 ggtaaatcga cgattcaagc attcatccat tcgatattat ttggatttcc aactaaaaag
     9361 tctaaagagc caagactaga accacgtcta ggtaaccaat acggtggtaa attagtactt
     9421 attcttgatg atggcttaga gattgaagtt gaacgaatta aaggcagtgc tcaaggtgat
     9481 gtgaaagtat atttacctaa tggtgctgtg cgtgatgatg cttggttaca aaagaaactt
     9541 aattatattt ctaaaaagac atatcaaggt atcttttcat ttgatgtact agggcttcaa
     9601 gacattcata gaaatctaaa tgaaaaacaa ttgcaagatt atttattaca agcaggggct
     9661 ttaggatcaa ctgaattcac gtcaatgcgc gaagtgatta atcgtaaaaa agatgaatta
     9721 tataaaaaat caggtaaaaa tccgatcatt aatcaacaaa ttgagcaatt aaaacaacta
     9781 gaaagtcaaa ttcgtgaaga agaagcaaag ctagaaacat atcatcgctt agtagatgat
     9841 cgagataaat catcacgtcg attagagaat ttaaagcata atttaaatca attatcaaaa
     9901 atgcatgaag aaaaacaaaa agaggttgct ttacatgatc attcacaaga atggaagtct
     9961 ctagaacaac agttaaatat tgagccaatc acattcccag aaaaaggtgt ggatcgttac
    10021 gaaaaagcac gagcgcataa gcaatcgtta gaaagagata ttggtttaag aaatgagcgt
    10081 ttagctcaac ttaaagaaga agcgactcaa ttagagccag ttaaacaatc tgatattgac
    10141 gccttcatta gtttgaatca acaagaaaat gaaattaaaa ataaagaatt tgaacttact
    10201 gcaatcgaaa aggatattgc gaataaacaa cgtgataaag atgaattgca atcaaatatt
    10261 ggttggtctg aaacgcatca tgacgtagat agttcagagg caatgaaaag ttatgtcagt
    10321 gagcaaatca agaataaaca agaacaagct gcatacatta aacaattaga acgtagttta
    10381 gaagaaaata aaatcgaaga taatgcggtt catagcgaac tagattctgt tgaagaaaaa
    10441 atagttcctg aagagacttt tgaaaagaaa aaagaatact cacaacaagt cattgaatta
    10501 aacgaaaaag agaacttgta tagcaaattg aaagaacgtt tcgaaattga gcaacaagaa
    10561 aaacaaaaac gtcaaaaatt gttacgaaca acgtttattc ttttaacatt agtaggtatt
    10621 ggtttaactg ccttctcatt tatttcaaac aacatgttgt ttggtattat tttcgcagta
    10681 ttaactttag tatttgttat cggtatcatc atgtctaaat caaaagaagt agattatagc
    10741 gaggccatca cagatgaaat cgaagaaatt aaagcacaac tagccatttt agatgaaaat
    10801 tatgacttag attttgatct tgatgagcaa tatcgcattc gtgatcactg gcaacaagct
    10861 ttgaaaaata aagatatttt agaagagaaa cgtcagtata ttgaaggtcg tttaaatgat
    10921 gcgaaagggc gtcatgatga attacaatcg acagttgaga acgttaagga tgaattatac
    10981 ttatcatcta aaatttcaaa tgatttaatt gttgatagta tttctacaat ggcgaatatt
    11041 aaagcattag atcaacatat cagtgattta aatcaacaac gccagcaatt ggttcaagaa
    11101 ttagatacat tctacaatca tgctgaagct gttacaaaat cacaatttgt ttatttcaac
    11161 aaattatctt tattccacga tgtgcaacaa tggttgaaat cagctgaaga cacgaatgaa
    11221 aaatggcgta ttaatgctga aaataccaaa cttgttacaa atgaattaaa tcatttaaat
    11281 gctcaattag aagaaaataa taaagaaatt acagcactat tcgattttat caatgttggt
    11341 actgaagaag atttttacca acatcatgaa gattaccaaa cttatactag taatttgagt
    11401 cgttttaatg atttaactaa atatcttgag aaccaaaact attcttatga attaagttct
    11461 agtttaagtg aaaaaacaac tgcacaactt gaagaagaag atcatttatt ggctactcaa
    11521 gttgacgaat ataatgagca atatcttgaa atgcaagcac aagtcagtga tttaagtgca
    11581 caaatcaatc acatggaaac tgatacaacg cttgctaatt taagacatga atatcacagt
    11641 cttaaaaatc aacttaatga tatcgcaaaa gattgggcaa gtttaagtta tttacaaagt
    11701 ttagttgatg aacacattaa acaaattaaa gataaacgtt tgcctcaagt tattaatgaa
    11761 gcggtagaaa tattgaagca tttaacagat ggcagatata cgatgattaa ctataatgaa
    11821 gattcaatta cggttaaaca tgttaatggt caattatatg atcctgttga actaagtcaa
    11881 tctacaaaag aattacttta tgtagcttta cgtatcagtt taattaaggt actaagacca
    11941 tattatccgt tcccattaat tgttgatgat gcatttgttc attttgataa aaaacgtact
    12001 gaaaaaatgt tgaattattt acgatcatta tcagaacact atcaagtact atactttaca
    12061 tgtgtaaaag ataatattgt tccatcaaaa gaagtgatta cattaaacaa aatagaggaa
    12121 ggcgggaaac gatgagaaat atagagaatc taaatcccgg agattcagtt gatcactttt
    12181 tcttagtgca taaagctaca cagggtgtaa cagcacaagg taaagattat atgacattac
    12241 atttgcaaga taaaagtggt gaaattgaag cgaaattttg gacggctaca aaaaatgata
    12301 tggcaacaat caagcctgaa gaaattgtac atgttaaagg tgacatcata aactatcgcg
    12361 gaaataaaca gatgaaagtc aaccaaatta gactagcgac aactgaagat caattaaaaa
    12421 cagaacaatt tgtagatggt gcacctttat caccggcaga aatacaagaa gagatttctc
    12481 attatttgct agatattgaa aatgctaatt tacaacgtat cacacgtcat ttattgaaaa
    12541 aatatcaaga acgattttac acatatccag ctgctagttc tcatcatcat aactttgcga
    12601 gtggcttaag ctatcatgta ttaacgatgt tacgtattgc aaaatcaatt tgtgacattt
    12661 atccattgtt aaacaaaagt ttgttatata gtggtattat tttgcatgat attggtaaag
    12721 ttagagaatt gagtggtcct gttgcgacgt cgtatacagt cgaaggtaac ttattaggac
    12781 acatctcgat tgcgagtgat gaagtagttg aagcagctcg tgaattgaac attgaaggag
    12841 aagaaatcat gttgttaaga catatgattt tatctcatca tggtaagtta gagtatggtt
    12901 ctccaaaact gccatactta aaagaagcag aaattttatg ctatatcgat aatatcgatg
    12961 ctagaatgaa tatgtttgaa aaggcatata aaaaaactga caagggtcag tttacagata
    13021 aaatatttgg tcttgaaaat cgtagattct acaatcctga atcactcgat taagaattaa
    13081 aagatatcgg acagatgagt atactgaaac atgagctcat ctgtccgatt tttaatgcga
    13141 aaaaaccgag cggccgtggt atgatttttg aaccacggtc gctcggtttt gtgttattgg
    13201 ctcatgccgg attgtccgcc ttgtgcgcca ccttgtttaa gtttttcagg gtttaagatt
    13261 ttatcttcga caactgattt aatatcacga tctttaaagt caacatcgta ttcttttaat
    13321 agatctttgt atgcatcagt caataatttt ggattttttt gtactttctg atcgactaat
    13381 ttttctttca ggctttgttt ttcactgtta aagtctgttg gtttatcagc tttaataata
    13441 tgatatccaa agcttgattt aacaacctct gatacttcac catctttaag cttaaatagt
    13501 gctttttcaa aatctttatc agtttgtcct ttaagaacat aacctaattc gccatctttt
    13561 ttagctgaac cagtatccat tgattctttt ttagcgattt caccaaattt acttggatct
    13621 tttgaaactt ctttttgaat ttcttcagct ttttgtttcg cttctttatc atctaagcct
    13681 tctttgtcgc ttttcttaga tttaacttta attaaaatgt gtgaagcttt cttgctgtct
    13741 tctttaattt cagaatcaga gattttaatt ttatctgata gtaattcttt atgataagca
    13801 gcagtacgta aattttcttt atatttatcg gctgttaaac cttgctgttg aagggccttt
    13861 tcaaatttat ctttaccgcc gtattgcttt tgcatttttt caatttgttc gtcaatcttc
    13921 ttatcattaa ctttattttt atatttatca gctaaaattt tatttaacat ttcagtaaat
    13981 gatgcatttg caatttgatc tttaccgatt tttttcattg tatctgcaac tgttacgtct
    14041 ccagctttag aagaaattaa tgtattttct ttagagtctg tggcactagc gccacaagcg
    14101 cctaataata aagcactagc tgttaccgga acgattaatt tgtttatcat cttcatagtt
    14161 gaaactcctt tgtaagttaa tcatcgtgaa tataacatag ttttatatta tcaccaatca
    14221 ataacccatg acatcagact agagcattag gtttaggggt agaaattgat gaaaataaaa
    14281 aaacaaggtc tatatacatt tgattgtaag ctattacata gcctaaaaac aaataataac
    14341 accttgctat aattattttg ttataaatga tatggatttt taattttttt caaaatatta
    14401 atacctgtac tatgttcttc tatatattgt ccataattag aaaatataat tatttttaaa
    14461 tataataaat ctgtaatgga aataccgaaa ttcaatgcca ataagaacat aaaataatgt
    14521 ccatattgcg ggaatgaaat tgttagataa acgaacattg cagataagat aattaacggc
    14581 aatattaagt taatgcaaaa ataaaatttg tgtacaggtt gattgacgta ggtattatag
    14641 aatataagcc attttctttt agttaactta tgaactttaa acgattttct atatggtaag
    14701 aaaaataata aatgtatact tttatgtaat ggatacgtca taaatacaag taataaaaag
    14761 atgagaaaat gtctatctga taatggcgta ttagataaaa aatacattac ttcataacta
    14821 actaaaaaca taatgatggt tgcaactgca ctcataaatg caattctagg caaaccaaat
    14881 cgtgcattga tatcaatttg tcttttacat aaaaacatat cttacgcctc atctcttttc
    14941 aatataagtt agattataac taactcaggt tcagtaattc aatagtaaca tatcatgtta
    15001 gaagaattgt tatataagaa gaaaggagcg acgtattgtt tgatttgaaa cgcgaaagtg
    15061 gggattggta aaattgtctg attgactagc gtagaagtag aatgcagaac aaatttctgc
    15121 gaacacatat tcttgctaat aaagcatcac ttatgaaaaa ggcgcgtgtg acatgagtat
    15181 ttgaaaactt gaataatata aaaaatggat atggcagggc atatgcaaaa gcatcttttg
    15241 accaactaag ttatacaaat gctaaaaata tagtgatatg taattataat aaacaccaga
    15301 tttataattt gtgcaagtaa ggtatatgta atgtgtatat aaggttcggc tcaatgaaaa
    15361 tataagtatg ttgtttgtcg ataatgattt aagtaaagcg taatcaaagt gcaaggataa
    15421 cacaatgaat cctaagcaat gttttcaatc aattcataaa tatgatttaa gcgaaaatca
    15481 ctacaaacaa tgtcttccta taatttgtaa acataagttc atttgagttt taaaaaagag
    15541 atgacaagaa atatctaatt tctaatgtca tctcgtttat ttggcattta attttaaaaa
    15601 ataaatatgc tgttactaat aaaaatagtt gtgatcgaga acgtaacctg ctacttagaa
    15661 atttcgtttc caatatcctc gccacgattt tgtaaatttt cgatgtgtga ctgtaaacgt
    15721 tcaatattag gattaatgtc agatttgaag tttccaagta atgacttaat ttcaccacca
    15781 attgaactac caaattccgc actttcgttt ttaatttgaa caccatagtt taaaatgtca
    15841 ttaaaacttt gtttaatcgt ttcaaattca cgttgtagtt ctgattttga accagtaggg
    15901 gcagtacgat cgatcgtgtt gttcttgaca cttttgtcgt cacgtccttg aagtgcaact
    15961 acaaaaccag ctgctacgcc aacaccgata ccgaatagaa tgcgtgatgc tttcatttat
    16021 attctcctca cttttaacat ctgttatctt gataactcat tttgtttaaa attgctaacc
    16081 ttgatgttat cacttcaaac gcaattgtta taatattaat catattatac ccgttcgagc
    16141 ttagattaag catacattta aaattgtgct tgaatttgtt cagcaatttg ttgttttgca
    16201 tcgttatcta aaatgtcttc atgtgtttcc cacttataac caaatccatc aatatcattt
    16261 tcgtatcgag gaattaagtg gaaatgaata tgaaatacag attgatctgc aaactcacca
    16321 ttattttgaa taatgtttaa accatcagga ttaaatgcac gcttaatagc atttgctact
    16381 ttaggtaatg ctgcaccgat atgtttcatt gtttcttcat cagtttcaaa gatattagca
    16441 gaagcttttt taggaattaa taacgtatgt cctttagtaa cttgtgatat atctaaaaag
    16501 gcatagacat aatcgtcttc atatacttta aagctaggaa tttctccagt taaaattttg
    16561 ccgaaaattg tttctgacat gatgactaca ctccttttca actagtatta ccattcatta
    16621 taacattgtc gctaaaaatt cgatattagg acatgtgtat ttaatatgag cattttataa
    16681 tttttggtac aatataagtc atggaggtgc cttatgacag ttaaagtaga acagcttaca
    16741 ggtggatatg gaaaacgccc tgtaattaaa gatataaatt ttgaattaaa caaaggcgaa
    16801 attgttggac ttattggcct taatggcgct ggtaagagta caacgattaa acatatgcta
    16861 ggattgctta cacctatgga aggatctcta tcaatttcag atatcaacat taatgatgac
    16921 atagaggctt atagaagaaa gttatcttat attccggaat caccggttat ttatgaagaa
    16981 ctcacattag aggaacacat tgagatgaca gcaatggcat atgatattga tcgtgatgaa
    17041 acgatgaatc gagcaatgcc attattaaag acattccgtt tagaaaatga gttgaaagtt
    17101 tttccgagtc atttttctaa ggggatgaaa caaaaagtca tgattatttg tgcgtttatc
    17161 gttaatcccg aattgtatat tatagacgaa ccattccttg gcctagatcc attaggaatt
    17221 caatccatgt tagatttaat ggttgaaaag aaaaacgaag gtagaaccgt tctaatgagt
    17281 acacatattt tagcaacagc tgaacgttac tgtgatcgtt ttatcatact agacgaaggt
    17341 gaagtcgttg catttggaga tttagaagca ttgagacaac aaactggctt acataatcaa
    17401 acattagacg atatatatat tcatgtgacg caaggtggag atgtacatgc gtaaccaagc
    17461 taccacatta tttaataaaa gattacatgc ccttcgtaaa gagaaaaatt attataataa
    17521 gtttatattt aatggtcatt tcatggtctt tttacttatt ttgctgggtg catttatttt
    17581 tggatatggt gaatggttaa aacatattcc aacgaatatc aatttttcat taatagcagc
    17641 agtgattgtt gcgttgacat ctatttttcc aatgcgacca ctgttaaaag aagcggataa
    17701 aatcttttta ttaccatttg aaaaacacat gtctcaattt atgcgtcatg cgattttata
    17761 tagttacttt gcgcgcatat taatccaact cattattgtt attgtaatgt tcccgttgtt
    17821 ttacaatatt aatcaacata atgtggcatt ttatatttgt tttggagtca gtgcattaat
    17881 ttttccatat gttggtttac gcttaagatg gcaatggtat caatcgggac ttaaaacatg
    17941 gcaagttaat ttaatatcat tcatcacctt tgctctaaca tattacttac tattagctcc
    18001 aaaatggtac atcgcatttg tgatggttgc tttgcctgtg ttaatagaat ttttagttaa
    18061 aaaatataaa ccaggctttt tgtatccttg ggagaaaatg attgcaatcg aacatcgaca
    18121 tcatatgaat tattataaat ttgttaatat gttcaccgat gtaaaacatt tgaaagaatc
    18181 tgcagtcaga agaagttatc tagatatact tttaccagtt ccaaagggta gtaaatttaa
    18241 ctctaatgcg atgtatttat tcttatttat tagaagcttt ataagaggga gagacgcatt
    18301 taatattatc tttaggttag taattattgc agttttatta atggtttggt tatcatatcc
    18361 attagtgaca gcaattattg ggtgtttatt tgtatatatt atattgttac aaatggcaca
    18421 attttattca cagcaagcat atggcttatg gcctcaagtg tggcctgtac ctgaagagaa
    18481 ggtaatcaaa gggtatgaac agtttttata tagattaatg tttgttattt gtactgtgtt
    18541 tgcagtgaca tttattataa aacatatgac gttattctat gttgtactca ttttctatat
    18601 tgtaggttta ttaacgatta gaagtattat taaaaagttg aaatatcagg aaacattatt
    18661 acgagactaa aaaacttcca ttggcatgta tgttgtaaag gtgcatgtaa tgttgaacgc
    18721 caaatgatac ggcgttcaga ttacattagc atctatacgt taacagcata accaatggaa
    18781 gttttcttcg aatctattct tttattgggt atagatatct ttcaaaataa cttgaatgtt
    18841 gtccgcttga accaaagtaa tgacttaatg cgtcttttga aaaatggtca ttaaaggcat
    18901 cagattgctt aaagtcttcg tatgcatgtc gatcagcaaa tccgaaataa attttgtatg
    18961 ttgtaccttt agcaggtctt aacaaacgat agcttttaaa gccaccaaag tttctgaaat
    19021 tatcgtctac actaatcagt ttcttttcaa gttgatatgc atgatcttct gttgatggaa
    19081 tgaagattgc acaatagaaa tgatgttcac tgaattcacc aatttcttta ataacttcat
    19141 atattgaagg tgattttaaa acagtctcac catcagtttc ttcaaaaata actgaagtat
    19201 ctgatgctga aaattggaat agttgatggg tcgggttatt gatttttatc tgatgtaaaa
    19261 atccataagt gccataagat gtatatagtt tcttcatatg aaaggtcccc cttatgtttt
    19321 tatttattat acgatgataa actagtcatt accactatta ataattgatt aaaattacta
    19381 taatgaatct tgcgagatat ttagaagtaa tgatacaaaa taatggactt tagaatttaa
    19441 atttacaata aatagttcta tgattaaatg tcagttttat gacatttatt tattgaaaat
    19501 acgaacgaat gagcgatatg ataatataga taagaatgat tttaatttag gaggccttta
    19561 tggtgcataa taaaaacaat acaattttaa aaatgatcaa aggtgaagaa acatcacata
    19621 cacctgtttg gtttatgcga caagctggcc gttcgcaacc agaatatcga aaattgaaag
    19681 aaaaatattc actattcgat attacacatc agccggagtt gtgcgcttat gtaacacatt
    19741 taccagttga taattatcat acagatgcag caattttata caaagatatt atgacaccat
    19801 taaagccaat tggtgtcgat gtagaaatta aatcgggtat tggtccagtg attcataatc
    19861 caatcaaaac aattcaagat gttgagaaac tttctcaaat agaccccgaa cgagatgtac
    19921 catatgtatt agatacaatt aaacttttaa cagaagaaaa gttaaatgtg ccgctaatag
    19981 gatttactgg ggcaccattt acattagcgt catatatgat tgaaggcgga ccatcgaaaa
    20041 attacaattt tacaaaagcg atgatgtata gagatgaagc aacatggttt gctttaatga
    20101 atcatttagt tgatgtatct gttaaatatg taacagctca agtcgaagca ggtgccgaat
    20161 tgattcaaat tttcgattca tgggtaggtg cattaaatgt cgaggattac agacgttaca
    20221 ttaaaccaca tatgattcga ttaatcagtg aggttaaaga aaaacatgat gtgccggtaa
    20281 ttttattcgg tgtaggtgcc agtcatttaa tcaatgaatg gaatgattta ccgattgatg
    20341 tattaggcct agattggaga acgtctatta atcaggctca acaattaggc gttactaaaa
    20401 cattacaagg gaatttagat ccatcaattt tattagcacc atggaatgtc attgaagaga
    20461 gattgaaacc aatattagat caaggtatgg agaacggtaa acacatcttt aatttaggac
    20521 acggtgtatt cccagaagtg caaccagaga cgttacgtaa agtaagcgaa ttcgtacaca
    20581 catatacaca aagataatca tagttttaca ttgaagcaca tgaaagataa ttcatttaaa
    20641 ggaaggtatt atcaatgact aaaaaaatgg gattattagt tatggcttat ggcacacctt
    20701 ataaagaaag tgacatagag ccatattata cagatattag acatggtaaa cgtccatctg
    20761 aagaagaact tcaagatttg aaagatagat atgaatttat aggtggttta tcaccattag
    20821 caggtacaac agatgaccag gctgatgcgc tagtttcagc attaaataaa gcatatgcag
    20881 atgttgaatt taaactatac ttaggattaa aacacatttc accatttatc gaagatgcgg
    20941 ttgaacaaat gcacaatgat ggcattactg aagcaatcac ggtagtacta gcaccacatt
    21001 attcttcatt ttcagtagga tcatatgaca aacgtgctga tgaagaagct gcaaaatatg
    21061 gtattcaact tacacatgtg aaacattatt atgaacaacc taaatttatt gaatattgga
    21121 cgaataaagt caacgaaaca ttagctcaaa taccggaaga ggaacataaa gacacggtat
    21181 tagttgtttc ggcacatagt ttgccaaaag gtttaatcga aaagaataat gatccatatc
    21241 cacaagaact agaacatact gcgcttttaa ttaaagaaca atctaatatt gaacatatcg
    21301 cgattggttg gcaatctgaa ggtaatacag gtacaccttg gttagggcca gatgtacaag
    21361 atttaacacg tgatttatat gaaaaacatc agtataaaaa ctttatatat acgccagtag
    21421 gttttgtatg tgagcattta gaggtgcttt atgacaatga ttatgaatgt aaagtagttt
    21481 gcgatgatat tggtgcgaat tattatcgtc caaaaatgcc gaatacacat ccattattta
    21541 tcggtgcaat tgttgatgaa atcaagtcta tattttaatg acgaaagaag cgtgaaacgt
    21601 tgtgactaaa tcagtggcta ttataggagc ggggataaca ggtttatcaa gtgcatattt
    21661 tttaaaacag caagatccta atattgatgt aaccatcttt gaagcatcga atcgtccggg
    21721 gggaaagatt caatcgtatc gtaaagatgg ttatatgatt gaactagggc ctgaatctta
    21781 tttaggtaga aaaacgatta tgacagaatt agcgaaagat attggattag aacaagatat
    21841 tgttacaaat acgactggac aatcatatat ttttgcgaaa aataaattat atccgattcc
    21901 aggtggttca attatgggta ttccaacaga tattaaacca tttgttacta caaaattaat
    21961 atcgccactt ggtaaattaa gagcagggtt tgatttatta aaaaaaccta ctcaaatgca
    22021 ggatggtgac atttctgttg gtgcattttt cagagcaaga ttaggtaatg aggtacttga
    22081 gaatttaata gagcctttaa tgggtggtat ttatggtacc gatattgata aattaagttt
    22141 gatgagtacg tttcctaatt ttaaagaaaa agaagaggca ttcggaagtc tgataaaagg
    22201 tatgaaggat gagaaaaata agcgtctgaa acaaagacaa ttatatcctg gcgcaccgaa
    22261 aggacaattc aaacaattta agcatggttt aagctcattt attgaagcat tagaacaaga
    22321 tgttaaaaat aaaggtgtga caatacgcta caatacgtca gtggatgata taattacatc
    22381 tcaaaaacaa tataaaattg tttacaatga tcaactagaa gaagtgtatg atggcgtatt
    22441 agtaacgaca ccacatcaag tgtttttaaa ttggttcgga caagatccag catttgatta
    22501 ctttaaaacg atggatagta cgactgttgc aactgttgta ttggcatttg atgaaaaaga
    22561 cattgaaaat acccatgatg gtactggctt cgtaattgcg agaacgagtg atacagacat
    22621 taccgcatgt acttggacat cgaaaaaatg gccatttact acaccggaag gtaaggtttt
    22681 gattcgtgcg tatgtaggta aaccaggtga tactgtggtt gatgatcata cagataatga
    22741 attagtatcg attgtacgta gagatttaag tcaaatgatg acatttaaag gtgatcctga
    22801 atttacaatt gtcaatcgtt tgccgaaaag tatgccacag taccatgtcg gtcatattca
    22861 acaaattaga cagattcaag cacatattaa acaaacatat ccacgactta gagtaactgg
    22921 tgcatctttt gaagcggttg gactacctga ttgtattacg caaggtaaag ttgctgctga
    22981 agaagtaatc gcagagttgt aagttaattg ttggatggtg ttggtgtagg tgcgacgcct
    23041 ttagataatg tttgatgaaa tatagtcatt tttaagttat ataatggtgg aagtaatgtg
    23101 taaatataga tactttcagc gggaattatt tttgtgaaat tgaagcagag acaaaggttt
    23161 agctgttttt gaatgatatt gaaatgatga caatacactt taattagaaa tgtcactttg
    23221 cgatataaca tttctaatta aggtgttttt attttacaat tataggtaat cagtcatttt
    23281 tttaaaaata gaaaagaagt agtaagttga aaggagagaa tgagatgaca gatgtaatta
    23341 ttgtacattc tatgcatgga aatagtagaa atcattggta tcagtggttg gagcataact
    23401 taactttaga aggatatgac gtaacattat ttaattttga atcccctgaa gcgaagacag
    23461 tggatcagtg gattgaagct atgacgaaac aaattaatgt acgcaaaaag gatacatatt
    23521 ttgtaacaca tggtttaggt tcaatcacag ctttaaaata tattgaaatg attgatcagc
    23581 cgattgaagg ctttttcagt atagcgggct ttaaagaaga tgcagaaaat atagatttag
    23641 atattgattt aagtaatgta accattgatt acgataatat taaaaagaaa gttgacaatt
    23701 tttacggatt gagttctaaa aatgacaaat acgtttcata taaagagacc cagcgtttaa
    23761 tgaatgcatt agaggggaac ttgcgaatcg tggaagatgg agggcacttc ttagaagagg
    23821 atggcttcga aacatttaca gcgttgcaag atcggatgca agattacatg acacgatagt
    23881 tagtgtatgg gttgtgttat gttgtgtcag taggtagctg ctgattttga aaaattgttg
    23941 taattgaggc gtcaagcaat gtcttagggc tacaatgtta tattggtagt cgctgactga
    24001 aagaatatgc ggtgtaacaa gcttttataa tttctttttc agaaggtata ggcgttgtaa
    24061 actttataac tcctatacct ttttaaatgc tttaacacta tttatgacaa ctattagaca
    24121 cttatatgat aaaagggaat aagaagcgtt tttttacaca aaaaactttg aaaatgtcgt
    24181 tgacgtacac tacaaaaatg aatatactaa tatttgtgtt caacagagaa catgttgcta
    24241 cgatatttaa gtaattattt aagagataac atttaaatta aattcaaaaa gttcttgact
    24301 ttaggttttt gattatggta tattaattag gtaattcaaa ataatattag cgcgggatag
    24361 agcagttcgg tagctcgtcg ggctcataac ccgaaggtcg gtggttcaaa tccgcctccc
    24421 gcaatacata gtttttaatt taataggtct cgtagtgtag cggttaacac acctgcctgt
    24481 cacgcaggag atcgcgggtt cgattcccgt cgagaccgcc attattatta ccattacggt
    24541 tcagtagctc agttggtaga gcaatggatt gaagctccat gtgtcggcag ttcgactctg
    24601 tcctgaacca ttcttaattc atggcggttg tggtgaagtg gttaacacat cggattgtgg
    24661 ttccgacatt cgagggttcg atccccttca gccgccccat aatcgtttac attagcgggt
    24721 gtagtttaat ggcaaaacct cagccttcca agctgatgtt gtgggttcga ttcccatcac
    24781 ccgctccatt attttctatt attccacagt agctcagtgg tagagctatc ggctgttaac
    24841 cgatcggtcg taggttcgag tcctacctgt ggagccatgg ctccttggtc aagcggttaa
    24901 gacaccgccc tttcacggcg gtaacacggg ttcgagtccc gtaggagtca tacaagcaga
    24961 agtgaaatat cgcttctgtt tttttattac atatttattg ttgaggaagg ttgtccgagc
    25021 tggccgaagg agcacgcctg gaaagtgtgt aggcgtcaca agcgtctcaa gggttcgaat
    25081 cccttacctt ccgtaaaggc gcttaaattg gttttaccca ttttaagcgc tatttttatt
    25141 ttggactcaa tcccttgata tatctgcatt tgagctatta tcctcatttt tacacttctt
    25201 atttatttat atccatttaa aattttttag ccacaatgtg actaattttt gatgaataat
    25261 cctaatttta gtcacaagat tttgaagttt agtcacaaaa caaatcattc agattttttt
    25321 ctataaattt agtttcatta tcttcaaatt ctttaatcaa atgtgaatat gttttaagcg
    25381 ttgtatgtac atctgcatga cctaatcgtt ttgaaatgta atgtttagct gttacttaaa
    25441 gaaaatcaaa tattatatat acgcttgaaa aaataaaata ataatattat aatataatca
    25501 atcggcatct acgtgcggat tataaagtaa agcgtagcat aaccaggagt tagtcgattt
    25561 ttaataaatt cgactaaatt attaataaag gctaaagtaa ttagtttact ttagccttta
    25621 ttgcatttgt aatttataga actgcgaaca ggtgactact gttaatttat gtgattaaag
    25681 tttcaatgat atttgattta ttagaccatt tcaaaatcgc aaggttctca tgttctgcta
    25741 cagtttgcgg tctttggaag atatatcatt tactattatt aacatgatat attctaattt
    25801 gtttgtggat gggctgtttg gtttgatatc gcggtaaata taagcatcat tagttatatt
    25861 gttgaaatct aagttgaatt tattatcata cagcaatgtg ccaattaggg acatttttaa
    25921 ttttgaactc tacattttgt tgagagtatc tggttatatt tgcaaaatgg tatgaacatt
    25981 tatcatcact tattaagttg tcttgatgat tgaatgaccc agtatttatt cagttcgaga
    26041 agattgagag tgttaaatcg tattcgagta taatttcgat tctagtcata ccattttcat
    26101 aaagtttagc catttgtaac ttaaactatg aactaaataa tcttctgact cttgtcaaaa
    26161 taaaatcgcc tacccaaata gtttaacaat atctattctc atagaatttg ttcaactaag
    26221 tgtagacgat tctgcattat caataaggct attcaatttg ctatcttaag ctgtttcgaa
    26281 attttcaaat tcgtttcatt taatataaac attgatatta tcacgtctat atatattaaa
    26341 tcaataaacc tatcttttca tctaattcgc tataatctat tgtactaata tagttttcat
    26401 gaagaagatt tttattatgt tcatttattt ctttttttga gtcatttaaa ataaaaataa
    26461 actcattttg cctgtgtctt tttatttttt tgcttgcata gcatccgtta ttttcgactt
    26521 aataatcata gaattattag ggttagaaat agcattgata aatttttctt tttgattttt
    26581 cttagcgcta ataaaaaagt cgaaattatg aacaacacca ctgcttccta taataggtaa
    26641 atctcttccg taataaatgt tatgttcatc caatttgttt gcaacatctt ctgtaaatat
    26701 gttctgtgac ttaggattag aaagtaagt
//
LOCUS       DAKTIO010000016        26166 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.016, whole genome shotgun sequence.
ACCESSION   DAKTIO010000016 DAKTIO010000000
VERSION     DAKTIO010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 26166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26166
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.016"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..1462)
                     /gene="sasF"
                     /locus_tag="O0293_000540"
     CDS             complement(<1..1462)
                     /gene="sasF"
                     /locus_tag="O0293_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasF"
                     /protein_id="HCY0279440.1"
                     /translation="MAKYRGKPFQLYVKLSCSTMMATSIILTNILPYDAQAASEKDTE
                     ITKEILSKQDLLDKVDKAIRQIEQLKQLSASSKEHYKAQLNEAKTASQIDEIIKRANE
                     LDSKDNKSSHTEMNGQSDIDSKLDQLLKDLNEVSSNVDRGQQSGEDDLNAMKNDMSQT
                     ATTKHGEKDDKNDEAMVNKALEDLDHLNQQIHKSKDASKDTSEDPAVSTTDNNHEVAK
                     TPNNDGSGHVVLNKFLSNEENQSHSNRLTDKLQGSDKINHAMIEKLAKSNASTQHYTY
                     HKLNTLQSLDQRIANTQLPKNQKSDLMSEVNKTKERIKSQRNIILEELARTDDKKYAT
                     QSILESIFNKDEAVKILKDIRVDGKTDQQIADQITRHIDQLSLTTSDDLLTSLIDQSQ
                     DKSLLISQILQTKLGKAEADKLAKDWTNKGLSNRQIVDQLKKHFASTGDTSSDDILKA
                     ILNNAKDKKQAIETILATRIERQKAKLLADLITKIET"
     gene            complement(1704..3062)
                     /gene="gtfB"
                     /locus_tag="O0293_000541"
     CDS             complement(1704..3062)
                     /gene="gtfB"
                     /locus_tag="O0293_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HCY0279441.1"
                     /translation="MIKLFDYFNDHSRKLYESFKASKLEEDLTIVLNDNGFLPDDVIS
                     PYQFFADNHNTENMKPRFFNQVTVPAFWEIKGNNNSATINDMGRLRGKIFYQSGERPR
                     IVSRVEWFDDQQRVRFVDYYSKNGIKFAQTVYDLNRKAILKKYMTAEGKEVIYENFVT
                     SDVILDWQGKSYFFPSKLAFALFFIKQLEITEHHFVINSLALPFSVLYNLPSKGSDVL
                     VWQEHCNGNVPGNMQLMCKGDMKRHCNIIIPDKNEYETMLNIADAKVQSRILQGGYLY
                     NYRSRNRYTKEIVILTNSDQLRNIKVLVETLPDFKFHIAAITEMSDKLMQLDQYANVH
                     LYPSINIDRVNELYQLCDIYLDINEGNEILNAVEQAFDYELLILGYRQTAHHAKVTLS
                     EHLFEHNDEITMESKDQLIQMLESLKDQQQFRDALLAQKAHAHEISREQFEQVFKQAL
                     ES"
     gene            complement(3055..4563)
                     /gene="gtfA"
                     /locus_tag="O0293_000542"
     CDS             complement(3055..4563)
                     /gene="gtfA"
                     /locus_tag="O0293_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HCY0279442.1"
                     /translation="MTIYNINFGIGWASSGVEYAQAYRAKLLRRTNQDTKFVFLDFIQ
                     SENIQTLTQNIGFKDQEVIWLYQYFSDISLAPTTYTLDDIERELGHDISFRERNGKIV
                     RLYFNGKSSFVTCYLQNEQKDIVDRAEFVINSMLVRKDFYSYTRIFSEYYAPADNKAK
                     LYMRQFYNQDGSIAYNEYIDGDSSIYVFEDAVLYNKTEFIAYFLQRLNLTRDDIVILD
                     RASDIGQAVLQHKGDSKVGVVIHADHYSNNMMSEQHILWNNYYEYQFSKAKYIDFFIT
                     ATDIQNHMVCRQFEQYQGYRPRVYTIPVGSIDALSYPTLSRKPYAMISASRLANEKHI
                     DWLVKAVIVAKRQVPELTFDIYGEGSEKTRLRKIIDTHRAQDYIRLLGHVKLDEIYND
                     YELFLSASTSEGFGLTLMEAVGSGLGMIGLNVNYGNPTFIRDGENGYLVPFDTDEDSV
                     DDVIAKLAHAIVMYFNNGPQTPHDISYEVAQQFMTQDIILKWETLVQEVLHD"
     gene            complement(4581..6971)
                     /gene="secA2"
                     /locus_tag="O0293_000543"
     CDS             complement(4581..6971)
                     /gene="secA2"
                     /locus_tag="O0293_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HCY0279443.1"
                     /translation="MKHKLDVTINELRLKSIRKIVKRINTWSDEVKSYSDDALKQKTI
                     EFKERLASGVDTLDTLLPEAYAVAREASWRVLGMYPKEVQLIGAIVLHEGNIAEMQTG
                     EGKTLTATMPLYLNALSGKGTYLITTNDYLAKRDFEEMQPLYEWLGLTASLGFVDIVD
                     YEYQKGEKRNIYEHDIIYTTNGRLGFDYLIDNLADSAEGKFLPQLNYGIIDEVDSIIL
                     DAAQTPLVISGAPRLQSNLFHIVKEFVDTLIEDVHFKMKKTKKEIWLLNQGIEAAQSY
                     FNVEDLYSEQAMVLVRNINLALRAQYLFESNVDYFVYNGDIVLIDRITGRMLPGTKLQ
                     AGLHQAIEAKEGMEVSTDKSVMATITFQNLFKLFESFSGMTATGKLGESEFFDLYSKI
                     VVQVPTDKAIQRIDEPDKVFRSVDEKNIAMIHDIVELHETGRPVLLITRTAEAAEYFS
                     KVLFQMDIPNNLLIAQNVAKEAQMIAEAGQIGSMTVATSMAGRGTDIKLGEGVEALGG
                     LAVIIHEHMENSRVDRQLRGRSGRQGDPGSSCIYISLDDYLVKRWSDSNLAENNQLYS
                     LDAQRLSQSNLFNRKVKQIVVKAQRISEEQGVKAREMANEFEKSISIQRDLVYEERNR
                     VLEIDDAENQDFKALAKDVFEMFVNEEKVLTKSRVVEYIYQNLSFQFNKDVACVNFKD
                     KQAVVTFLLEQFEKQLALNRKNMQSAYYYNIFVQKVFLKAIDSCWLEQVDYLQQLKAS
                     VNQRQNGQRNAIFEYHRVALDSFEVMTRNIKKRMVKNICQSMITFDKEGMPVIHFP"
     gene            complement(6961..7920)
                     /gene="asp3"
                     /locus_tag="O0293_000544"
     CDS             complement(6961..7920)
                     /gene="asp3"
                     /locus_tag="O0293_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HCY0279444.1"
                     /translation="MLKNKTFKVTWNYISQTTFMYGSKVSFSNGEVTFINPLMPSGLP
                     IHEWLMLKQFSKYKSAPSLPILRRGQHYKLHFDFDATPAGSVYFIIIFYNKNGTKLST
                     EIVKSNSITIQYPDEAYAYKIKMMNAASTSLIFRCLTITEMTHQYDLEYKSMRVTKIG
                     DDQYGNDMINVIIAEPSDTYPTISNDFLKLFGHVWLVERWMDDNIEGNIKQLKNDLQS
                     QDTLKAINLISYGSKSNVFATYVAQHLGCKVYRTSHEDDDLKGWLTEHVPGNHELKDT
                     NVEVYFKEEQDKHLNYMSRLMNPVRFLDYLDVSKLNGGEVNET"
     gene            complement(7898..9466)
                     /gene="asp2"
                     /locus_tag="O0293_000545"
     CDS             complement(7898..9466)
                     /gene="asp2"
                     /locus_tag="O0293_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HCY0279445.1"
                     /translation="MPRKFRVLQIGGDDLEPIFQHKKGVSWDYFDIGLFEFDSGYVEA
                     IEAIVEAEGRFDFIYIQAPYSETLTNLLQMISEPYNTYVDESFWSVEYEQDENVQKYV
                     VQPLHYRNIEERNNKLEAVSFSGQYGDKVSPKLALVHPNFKGDVVYQGNSELTLSGEF
                     GKEFKPIASWQNNLVYDKDKVIQIWPEFDIDGAVELQYTFRLIQTGADGALIEQIVLT
                     DDMLDSPLEIPAKPFDAYISVTVKARGNGTVHLGPIHKRWSRLDMGQFLLGGSRFVDS
                     QRQEFIYYFHPGDMKPPLNVYFSGYRTAEGFEGYYMMKRMNAPFLLIGDPRVEGGSFY
                     IGSSEYEQGIINVIDETLEKLNFKSHELILSGLSMGSFGALYYGAQLNPQAIIVGKPL
                     VNIGTIAEHMRLLRPEEFGTALDVLVSNEGDTSQASIQALNQKFWQTFQKKSLSQTVF
                     AIAYMQHDDYDPHAFQELLPVLTAHQARVMNRSIPGRHNDDSPTIASWFVNFYNIILE
                     DKFGRVQHAEKQNI"
     gene            complement(9456..11009)
                     /gene="asp1"
                     /locus_tag="O0293_000546"
     CDS             complement(9456..11009)
                     /gene="asp1"
                     /locus_tag="O0293_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HCY0279446.1"
                     /translation="MKYFIPAWYDDQRWWQDTTVPYYQLQNKTEFDDMISLMGMHLEN
                     DLDYQLIVLNHAPNLRTFLHRYDLYETKYSSVFDEIQGFSHHAPQAINYHHLKWPDDV
                     EFVYTPYLLKCVTSEQTYTNIYFSQEGYSIWFEEFERDQLQRRYIFDDRGYLSAIRYF
                     DDQGEASYQEYLTINGDCVLHEDLKNGRVTVSKRYQHHYQQTEYNNMAQLIEEKFQAM
                     IAQQIHEDDHVIVASDARHNRQIANHIPAKSLSYSFFKNRNETVSDEEYQSIVKNAHL
                     IVDSVQLERDLISHQEKYQRENTMIRITPFETRQSPNISSQLMETFIGVWIDGMSDAD
                     LQQMMQRLVDYIAQEDYYRLILLSRHQNDIPMWLRECITSVNEEYQAKQNADVNVSAL
                     MTPEDQDDIIAVKTIHAEHDVVEALRTLRLVIDMSKEPDLYLQISAISAGIPQINGQQ
                     TDYVSDYDNGRIINTVDELDDALNYYLFYLKNWNYAYAYSLKLIDAYASKNIINQLDE
                     LIEGENDAT"
     gene            complement(11020..12231)
                     /gene="secY2"
                     /locus_tag="O0293_000547"
     CDS             complement(11020..12231)
                     /gene="secY2"
                     /locus_tag="O0293_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HCY0279447.1"
                     /translation="MLKLLQQYEYKIIYKRMLYTCFILFIYILGTNISIVSYNDMQVK
                     HESFFKIAISNMGGDVNTLNIFTLGLGPWLTSMIILMLISYRNMDKYMKQTSLEKHYK
                     ERILTLILSVIQSYFVIHEYVSKERVHQDNIYLTILILVTGTMLLVWLADKNSRYGIA
                     GPMPIVMVSIIKSMMHQKMEYIDASHIVIALLIILVIITLFILLFIELVEVRIPYIDL
                     MNVSATNMKSYLSWKVNPAGSITLMMSISAFVFLKSGIHFILSMFNKSISDDMPMLTF
                     DSPVGISVYLVIQMLLGYFLSRFLINTKQKSKDFLKSGNYFSGVKPGKDTERYLNYQA
                     RRVCWFGLALVTVIIGIPLYFTLFVPHLSTEIYFSVQLIVLVYISINIAETIRTYLYF
                     DKYKPFLNQYW"
     gene            complement(12361..19176)
                     /gene="sasA"
                     /locus_tag="O0293_000548"
     CDS             complement(12361..19176)
                     /gene="sasA"
                     /locus_tag="O0293_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin SasA"
                     /protein_id="HCY0279448.1"
                     /translation="MSKRQKAFHDSLANEKTRVRLYKSGKNWVKSGIKEIEMFKIMGL
                     PFISHSLVSQDNQSISKKMTGYGLKTTAVIGGAFTVNMLHDQQAFAASDAPLTSELNT
                     QSETVGNQNSTTIEASTSTADSTSVTKNSSSVQTSNSDTVSSEKSEKVTSTTNSTSNQ
                     QEKLTSTSESTSSKNTTSSSDTKSVASTSSTEQPINTSTNQSTASNNTSQSTTPSSVN
                     LNKTSTTSTSTAPVKLRTFSRLAMSTFASAATTTAVTANTITVNKDNLKQYMTTSGNA
                     TYDQSTGIVTLTQDAYSQKGAITLGTRIDSNKSFHFSGKVNLGNKYEGHGNGGDGIGF
                     AFSPGVLGETGLNGAAVGIGGLSNAFGFKLDTYHNTSKPNSAAKANADPSNVAGGGAF
                     GAFVTTDSYGVATTYTSSSTADNAAKLNVQPTNNTFQDFDINYNGDTKVMTVKYAGQT
                     WTRNISDWIAKSGTTNFSLSMTASTGGATNLQQVQFGTFEYTESAVTQVRYVDVTTGK
                     DIIPPKTYSGNVDQVVTIDNQQSALTAKGYNYTSVDSSYASTYNDTNKTVKMTNAGQS
                     VTYYFTDVKAPTVTVGNQTIEVGKTMNPIVLTTTDNGTGTVTNTVTGLPSGLSYDSAT
                     NSIIGTPTKIGQSTVTVVSTDQANNKSTTTFTINVVDTTAPTVTPIGDQSSEVYSPIS
                     PIKIATQDNSGNAVTNTVTGLPSGLTFDSTNNTISGTPTNIGTSTISIVSTDASGNKT
                     TTTFKYEVTRNSMSDSVSTSGSTQQSQSVSTSKADSQSASTSTSGSIVVSTSASTSKS
                     TSVSLSDSVSASKSLSTSESNSVSSSTSTSLVNSQSVSSSMSDSASKSTSLSDSISNS
                     SSTEKSESLSTSTSDSLRTSTSLSDSLSMSTSGSLSKSQSLSTSISGSSSTSASLSDS
                     TSNAISTSTSLSESASTSDSISISNSIANSQSASTSKSDSQSTSISLSTSDSKSMSTS
                     ESLSDSTSTSGSVSGSLSIAASQSVSTSTSDSMSTSEIVSDSISTSGSLSASDSKSMS
                     VSSSMSTSQSGSTSESLSDSQSTSDSDSKSLSQSTSQSGSTSTSTSTSASVRTSESQS
                     TSGSMSASQSDSMSISTSFSDSTSDSKSASTASSESISQSAFTSTSGSVSTSTSLSTS
                     NSERTSTSMSDSTSLSTSESDSISESTSTSDSISEAISASESTFISLSESNSTSDSES
                     QSASAFLSESLSESTSESTSESVSSSTSESTSLSDSTSESGSTSTSLSNSTSGSTSIS
                     TSTSISESTSTFKSESVSTSLSMSTSTSLSDSTSLSTSLSDSTSDSKSDSLSTSMSTS
                     DSISTSKSDSISTSTSLSGSTSESESDSTSSSESKSDSTSMSISMSQSTSGSTSTSTS
                     TSLSDSTSTSLSLSASMNQSGVDSNSASQSASNSTSTSTSESDSQSTSSYTSQSTSQS
                     ESTSTSTSLSDSTSISKSTSQSGSVSTSASLSGSESESDSQSISTSASESTSESASTS
                     LSDSTSTSNSGSASTSTSLSNSASASESDLSSTSLSDSTSASMQSSESDSQSTSASLS
                     DSLSTSTSNRMSTIASLSTSVSTSESGSTSESTSESDSTSTSLSDSQSTSRSTSASGS
                     ASTSTSTSDSRSTSASTSTSMRTSTSDSQSMSLSTSTSTSMSDSTSLSDSVSDSTSDS
                     TSASTSGSMSVSISLSDSTSTSTSASEVMSASISDSQSMSESVNDSESVSESNSESDS
                     KSMSGSTSVSDSGSLSVSTSLRKSESVSESSSLSCSQSMSDSVSTSDSSSLSVSTSLR
                     SSESVSESDSLSDSKSTSGSTSTSTSGSLSTSTSLSGSESVSESTSLSDSISMSDSTS
                     TSDSDSLSGSISLSGSTSLSTSDSLSDSKSLSSSQSMSGSESTSTSVSDSQSSSTSNS
                     QFDSMSISASESDSMSTSDSSSISGSNSTSTSLSTSDSMSGSVSVSTSTSLSDSISGS
                     TSVSDSSSTSTSTSLSDSMSQSQSTSTSASGSLSTSISTSMSMSASTSSSQSTSVSTS
                     LSTSDSISDSTSISISGSQSTVESESTSDSTSISDSESLSTSDSDSTSTSTSDSTSGS
                     TSTSISESLSTSGSGSTSVSDSTSMSESNSSSVSMSQDKSDSTSISDSESVSTSTSTS
                     LSTSDSTSTSESLSTSMSGSQSISDSTSTSMSGSTSTSESNSMHPSDSMSMHHTHSTS
                     TSRLSSEATTSTSESQSTLSATSEVTKHNGTPAQSEKRLPDTGDSIKQNGLLGGVMTL
                     LVGLGLMKRKKKKDENDQDDSQA"
     gene            complement(19721..20413)
                     /locus_tag="O0293_000549"
     CDS             complement(19721..20413)
                     /locus_tag="O0293_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCY0279449.1"
                     /translation="MNYSPKQGVRSHGLPHDPFKSSTVPRPIGWISTVSKDGKDNLAP
                     YSQYQNLTWDPPMVMFAANQSVLGDHERKDTVKNAEETGWFVWNMATYDLREAVNLSS
                     KALPPEEDEFNFADVTKEKCIEAPGYRVKESPVHFECEYVQTIRIPTGDPVSTVDIVI
                     GRVAQVHIDDKVILDNGKLDIKSIKPIARLGYYDYTVVNEIFEMKAPAASKEELAGLE
                     GRNFDNQSDEKI"
     gene            20649..21107
                     /locus_tag="O0293_000550"
                     /pseudo
     CDS             20649..21107
                     /locus_tag="O0293_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226788.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21094..21396
                     /locus_tag="O0293_000551"
     CDS             21094..21396
                     /locus_tag="O0293_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279450.1"
                     /translation="MRTNDHITDLDAIELSSGWNYNSKLETGKNALLSDSLLRDIKVL
                     SSNAKGDADILETELRAVINSMSQNEGKSVDALKNSTASLKNNLTKLKEQLDKTTH"
     gene            21550..21909
                     /locus_tag="O0293_000552"
     CDS             21550..21909
                     /locus_tag="O0293_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279451.1"
                     /translation="MILRYATNLKLAKELYQASKPKLQEDKGMIELFEHTFGLQKELV
                     KYVGIAEATTAALYSASFINKNISRLASLSTIGILSVAAYKHFEAGQGKKGAQHALNL
                     LGLATLSLLDTFSCNKK"
     gene            complement(21994..22605)
                     /locus_tag="O0293_000553"
     CDS             complement(21994..22605)
                     /locus_tag="O0293_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCY0279452.1"
                     /translation="MYTFDAQSLTARENYKLLIGSIIPRPIAFVTTLNQDASVNAAPF
                     SFFNIVNNHPPMIAIAVQRAGGKRKDTALNIERTGDFVVHITDEDNVQDINETAAPLA
                     YGDSELSRTELSLLTSTTIKTPGIKDAKMRFECKLSQMILLGDVLDGADLIIGEIVTY
                     HIDDSIYEGDFKINPHALQAVSRLAGNDYAKLGGIFTIKRPEK"
     gene            complement(22684..23160)
                     /locus_tag="O0293_000554"
     CDS             complement(22684..23160)
                     /locus_tag="O0293_000554"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HCY0279453.1"
                     /translation="MAVVYVAGGCLWGVEAFFATIPGIIHTEAGRANGRSSKLDGPYD
                     GYAECVKLHFDDRMLTITDIMNYLFEIIDPYSVNQQGNDIGQKYRTGLYSCVDDHLIE
                     ARQFIERRKDRDQIAVEVLPLSNYIKSAEEHQQHLEKYPEDMHMCHISKDLLNKYK"
     gene            complement(23163..23663)
                     /locus_tag="O0293_000555"
     CDS             complement(23163..23663)
                     /locus_tag="O0293_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0279454.1"
                     /translation="MVLRKVIMQDLDQIIALENIGFSPEEAATREALKLRIEQIQETF
                     IVAEKNDEVIGYINGPVIKERYISDDLFKNVSTNNSEGGYISVLGLVVAPNYQGQGIA
                     GRLLNYFETLAKNHHRHGVTLTCRESLISFYEKYGYRNEGVSESCHGGIKWYNLVKNI
                     KKDDEV"
     gene            complement(23898..24665)
                     /locus_tag="O0293_000556"
     CDS             complement(23898..24665)
                     /locus_tag="O0293_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HCY0279455.1"
                     /translation="MIDIHNHILVDIDDGPKTIEKSIALLKQAKDEGVTSIVATPHHL
                     HPRYDNTFQQVLVKLAELRTHPEVQALDIKLFPGQEIRITDSILKGLDNGSIQGINRS
                     KYLLIEFPTGEVPHYTKQLFFEIQSRGYIPIIAHPERNRSIAKNPEILYELVANGALS
                     QLTSSSLVGGFGKNIQKLSLQFIECNLAHFVASDAHSCDQRPFLMQELFHNHKLKKYS
                     NDIEALLRNASSVINDNFVYLDRPTKPGKVKSFLKWF"
     gene            complement(24662..25354)
                     /locus_tag="O0293_000557"
     CDS             complement(24662..25354)
                     /locus_tag="O0293_000557"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HCY0279456.1"
                     /translation="MTNTRRSTSSLIVHEQPKSPISEKFRGIRSNIMFANPDSAVQSI
                     VITSEAPGAGKSTIAANLAVAYAQAGYKTLIVDGDMRKPTQHYIFNLPNNEGLSSLLL
                     NWSTYQDSIISTEIQDLDVLTSGPIPPNPSELITSRAFANLYDTLLMNYNFVIIDTPP
                     VNTVTDAQLFSKFTGNVVYVVNSENNNRDEVKKGKELIEATGAKLLGVVLNRMPKDKS
                     ASYYAYYGTDES"
     gene            complement(25371..26033)
                     /locus_tag="O0293_000558"
     CDS             complement(25371..26033)
                     /locus_tag="O0293_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapA"
                     /protein_id="HCY0279457.1"
                     /translation="MKEKFDLVKLLNILKKNIKLLLILPAICLVVSAALTFFVMPDKY
                     TASTQILVNMKKSSSDLAFQNVQSSLQSVNTYTEIIKSPRILDKVSREFDGQYSTAEL
                     NSFLKVTNQTNSQIITVSVTTGNKSESDKIVNKISKVFAHDMPKIMSVDNVTILSSAH
                     DNAVKVSPIVSVNLVISIIVGIVLAILIIFLKELLDKRIKTEEDVESQLGLPILGSIQ
                     KF"
BASE COUNT         7902 a         4892 c         4057 g         9315 t
ORIGIN      
        1 ctgtttctat tttagtaatt aaatctgcca gtaattttgc cttttgtctt tctatacgtg
       61 ttgctaaaat cgtttcaatt gcttgttttt tatctttggc attattcaaa attgctttta
      121 atatatcatc tgaagacgtg tcgccagttg atgcaaaatg tttcttcaat tggtcaacga
      181 tttggcgatt tgataatcct ttattcgtcc aatctttagc caatttatct gcttcagctt
      241 ttcctaattt cgtttgtaaa atttgagaaa tcaatagcga cttatcttgt gattgatcaa
      301 tcaatgacgt taataaatca tcactcgttg tcagagataa ttgatcaata tgacgagtaa
      361 tttgatctgc aatttgttga tctgttttac catcaacacg tatatctttt agaattttaa
      421 ctgcctcgtc tttattaaat atactttcta aaatgctttg tgtagcatac tttttatcat
      481 cagtacgtgc aagttcttcc aaaataatat ttcgttgact ttttatacgc tctttcgtct
      541 tatttacttc gctcattaag tctgattttt gatttttagg aagttgcgta tttgcaatac
      601 gttgatctaa agattgtaac gtattcagtt tatgatatgt gtaatgttgc gttgaggcat
      661 tacttttagc taatttttca atcatagcat gattaatttt atcgcttcct tgtaatttat
      721 cagtgagtcg attactatgg ctttgattct cttcatttga aaggaattta tttaacacaa
      781 catgtccaga accatcatta tttggcgttt tagctacttc atgattatta tctgttgtag
      841 acactgctgg atcttccgat gtatctttcg atgcatcttt cgatttgtgt atttgctgat
      901 tcaaatggtc taggtcttct aacgccttat ttaccattgc ttcatcattt ttatcatctt
      961 tttctccatg ttttgttgta gccgtttgtg acatatcatt tttcattgca ttaagatcgt
     1021 cctcgccact ttgttgaccc ctatcaacat ttgaagaaac ctcatttaaa tctttaagca
     1081 attgatctaa tttactgtct atatcacttt gaccgttcat ttcagtgtga gaacttttat
     1141 tgtctttgct atccaactca ttagctcgtt ttatgatttc atctatttgc gatgctgttt
     1201 tcgcttcatt tagttgtgct ttataatgtt ctttagatga agccgataac tgttttaatt
     1261 gctcaatttg acgaattgcc ttgtcaactt tgtctaataa atcttgctta gataatatct
     1321 cttttgtaat ttcagtatcc ttttcagatg cagcttgggc atcgtacggc aagatattcg
     1381 ttaaaatgat acttgtcgcc atcattgtcg aacacgataa ctttacatat aattgaaacg
     1441 gtttccctcg atatttagcc atcaacatac tccttcctca cttacttcct tcaaagaatt
     1501 acatactatt atatacctgt ttacaagaaa tttacactta tctatctagt tattgttgtt
     1561 agtaattatc tacttattac ttagcttata tttaagtaaa caaaacaagc atgacgtaat
     1621 atcatattgt ccatgtcgct aacatcatat tacgtcaaat cttttatatt aaatgatgtt
     1681 ttattttaga ctgctttttc cttttagctt tcgagcgcct gtttaaaaac ttgctcgaat
     1741 tgttcacgcg agatttcgtg tgcatgtgct ttttgtgcta ataaagcatc tcgaaactgt
     1801 tgttgatctt tcaaactttc taacatttgt attaattggt ctttactttc cattgttatc
     1861 tcatcattat gctcaaataa gtgctctgat aatgttactt tagcatggtg tgcggtttga
     1921 cgataaccta aaatcaacaa ctcatagtca aacgcttgtt ccaccgcatt taaaatttca
     1981 ttaccctcat tgatatcaag ataaatatca cataactggt atagttcatt taccctgtca
     2041 atattaatag atgggtataa atgcacatta gcatattgat caagttgcat tagcttatca
     2101 gacatctctg taatagcagc gatgtgaaac ttaaaatctg gtaaagtttc aaccaatacc
     2161 ttgatgttac gaagttgatc cgagttagtt aatattacaa tttctttagt atatctatta
     2221 cgactacgat agttatatag atatccgcct tgtaaaatac gagattgaac ctttgcgtct
     2281 gctatattga gcatcgtttc atattcgttt ttatctggaa taataatatt acaatgtcgt
     2341 ttcatatcac ctttacacat caattgcata tttcccggga cattaccatt acagtgttct
     2401 tgccatacca aaacatcact accttttgat ggcaaattat ataacactga aaatggtagg
     2461 gctagtgagt taataacgaa atgatgttcc gtaatttcaa gttgcttgat aaaaaataat
     2521 gcgaatgcga gctttgaagg gaaaaagtaa gacttccctt gccaatccaa tatgacatca
     2581 gatgttacaa aattttcata aatcacttct ttaccttctg ctgtcatata tttcttcaag
     2641 atcgctttac gatttaaatc gtaaacagtt tgtgcaaatt taataccatt cttagaataa
     2701 taatcgacaa atcggacacg ttgttggtca tcaaaccatt cgacacgact aacaattcta
     2761 gggcgctctc cactttgata aaatatttta cctcgtagac gtcccatatc attaattgta
     2821 gccgaattgt tgttaccttt aatttcccaa aaagctggta cagtaacctg attaaaaaat
     2881 cgtggtttca tattttctgt attatgatta tctgcaaaaa attgatacgg tgatataaca
     2941 tcgtccggta aaaagccatt gtcattgagt acaattgtta aatcttcttc caacttactg
     3001 gctttaaaag actcatataa ctttcgtgaa tgatcgttaa agtaatcaaa taatttaatc
     3061 atgtagcacc tcttgaacta atgtttccca ttttaaaata atatcttgag tcataaattg
     3121 ctgtgccact tcataagaga tgtcatgtgg tgtctgggga ccattgttaa aatacattac
     3181 aatggcatga gctagttttg cgataacatc atccacacta tcttcgtcgg tatcaaaagg
     3241 taccaagtag ccattttccc catctcgaat aaaggttggg ttaccataat tcacatttaa
     3301 tccaatcata cctagtcctg agcctaccgc ttccattagt gttaacccaa aaccttcgct
     3361 agttgatgca gaaagaaata actcataatc attataaatt tcatcaagtt taacatgccc
     3421 tagtaaacga atataatctt gtgcgcggtg tgtatcaata attttacgca gtcgcgtctt
     3481 ctcgctacct tctccataaa tatcaaatgt taattctggc acttgtcgtt tagccacgat
     3541 aaccgctttg acaagccaat caatatgttt ctcattcgct aaacgagatg cactaatcat
     3601 cgcatatggc tttcttgata atgttggata tgataatgca tcaatgcttc ccacaggaat
     3661 agtataaaca cgtgggcgat aaccttgata ttgctcaaat tgtcgacaaa ccatatgatt
     3721 ttgaatatct gttgctgtaa taaagaaatc aatgtattta gcttttgaaa attgatattc
     3781 ataataattg ttccatagta tatgctgctc actcatcata ttattactat aatgatcagc
     3841 atgaatcaca acaccaactt tactatcacc tttatgctgc aaaacagcct gaccaatatc
     3901 agaagcgcgg tctaatatga caatatcgtc tcgggttaaa ttcaatcgtt gtaaaaagta
     3961 tgcaataaat tccgttttgt tatacaacac cgcatcttca aacacatata tagagctgtc
     4021 tccatcaata tattcgttat aagcgatgga accatcttga ttataaaatt gtcgcatata
     4081 taatttcgct ttattatcag ctggtgcata atactcagaa aatatgcgcg tataactata
     4141 aaaatcttta cgtactaaca tactattaat tacaaattct gcacgatcca caatatcttt
     4201 ttgttcattt tgcagataac atgttacaaa tgatgatttc ccattaaaat ataggcggac
     4261 tatcttacca tttctttctc taaaactaat gtcatgacca agctcacgtt caatgtcatc
     4321 taacgtgtac gttgttggtg ctaaagaaat atcactaaaa tactgataca accaaataac
     4381 ttcttgatct ttaaacccaa tgttttgcgt taatgtctgt atgttctctg actgtataaa
     4441 atctaaaaac acaaatttag tgtcttgatt tgtacgtctc aataatttag cacggtaagc
     4501 ttgtgcatat tcaacaccgc tactcgccca gcctatacca aagtttatat tatatattgt
     4561 catgcgctac cccttttcat ttatggaaaa tgtataactg gcataccctc tttatcaaat
     4621 gtaatcatgc tttgacaaat atttttcacc attctttttt tgatatttcg tgtcataact
     4681 tcaaatgaat ctaaggcaac tctatggtat tcaaaaatag cgttacgttg cccattttgt
     4741 cgttgattaa cgcttgcttt taattgttgt aaatagtcga cttgttctaa ccaacatgaa
     4801 tcaattgctt tcaaaaagac tttttgaacg aaaatattat aataatatgc actttgcatg
     4861 tttttacgat tcaaagctaa ttgcttttca aattgctcta ataaaaatgt cactactgct
     4921 tgcttatctt taaaattaac acaagccaca tctttattaa attggaaact taaattttga
     4981 taaatatact cgacaacacg cgattttgtt agcacctttt cctcatttac aaacatttca
     5041 aatacatctt tagctaacgc tttaaaatct tgattctcag catcatctat ttctaaaact
     5101 cgattgcgtt cctcgtatac aagatctcgc tgtatactaa tgcttttttc aaattcatta
     5161 gccatttcac gagctttaac cccttgttct tccgagatac gctgcgcttt aactacaatt
     5221 tgcttaactt tgcgattaaa caaattactt tgcgataatc gttgtgcatc taatgaatat
     5281 aattgattat tttccgctaa attactatcg ctccatcgct taactaaata atcatctagt
     5341 gaaatatata tacaagatga tcccggatcc ccttgtctac cagaacgacc acgtaattgc
     5401 ctgtctacac ggctattttc catatgttca tgaataataa cagctaatcc acctaatgct
     5461 tcgacacctt caccaagttt aatatctgtg cctcgacctg ccatactagt cgcaacagtc
     5521 atggaaccaa tttgccctgc ttcagctatc atctgcgctt cttttgcaac attttgcgca
     5581 atgagtaaat tattaggaat atccatttgg aataatactt tcgaaaagta ttcagccgct
     5641 tcagcagttc tcgttatgag taaaaccggt cgccccgttt catgaagttc aactatatca
     5701 tgaatcatcg cgatgttttt ctcatcaact gaacgaaaca ctttatctgg ttcatcgata
     5761 cgttgaatcg ctttatcagt tggtacttgt acgactattt ttgaatacaa atcaaagaac
     5821 tctgattcgc ctaattttcc tgtagctgtc atacctgaaa atgattcaaa aagtttaaat
     5881 aaattctgga aggtaattgt tgccataaca cttttatctg ttgaaacctc cataccttct
     5941 ttcgcttcaa tagcttggtg aagtccagct tgcaacttag ttcccggtaa catacgacct
     6001 gtaatacggt caattaaaac aatatcacca ttatatacaa agtaatcgac attagattca
     6061 aacaaatatt gtgcgcgcag tgctaaatta atattacgca ctaggaccat cgcttgttcg
     6121 ctatataaat cttcaacatt aaagtatgat tgtgccgctt caataccttg atttaacagc
     6181 catatttctt ttttggtctt cttcatttta aaatgcacgt cttcaatcaa tgtatctaca
     6241 aactctttca caatatgaaa tagatttgat tgtaatcttg gtgcacccga aataactaat
     6301 ggtgtttgag cagcatctaa aatgattgaa tccacttcat caataatacc gtaatttaat
     6361 tgtggtaaaa atttcccttc cgcactatca gccaaattat caattaaata atcaaaaccg
     6421 agacgtccat tagttgtata tataatatca tgttcatata tattacgttt ttcccctttt
     6481 tgatactcat aatccacaat atcaacaaaa cctaatgaag cagttaaacc taaccattca
     6541 tataatggtt gcatctcttc aaaatctcgc ttcgctaaat aatcattcgt tgtaattaaa
     6601 tatgttcctt ttcccgaaag agcatttaaa tataaaggca tcgttgccgt taatgtttta
     6661 ccttcgcctg tttgcatctc cgcaatgtta ccttcatgca atacaatcgc tccgattaac
     6721 tgaacttctt taggatacat acctaatact ctccagctcg cttcacgtgc cactgcataa
     6781 gcttcaggta acaatgtatc tagtgtatca actcctgatg ctaaacgttc tttaaattct
     6841 attgtctttt gttttaacgc atcatcagaa tatgatttaa cttcatcgct ccatgtattg
     6901 atgcgtttca ctatttttct aatcgacttt agtcttaatt cgtttatcgt aacatctagt
     6961 ttatgtttca tttacttccc caccattcag tttcgataca tctaagtaat ctaaaaatcg
     7021 tactggattc attaaacgtg acatataatt tagatgtttg tcttgctctt ctttaaaata
     7081 aacctcgaca tttgtatctt ttagttcatg atttcctggg acatgttctg taagccatcc
     7141 ttttaaatca tcatcttcat ggcttgtacg atacactttg caacccaaat gctgagcgac
     7201 ataagttgca aaaacatttg actttgaccc ataactaatc aaattaatag cctttagggt
     7261 atcttgactt tgcaaatcat tctttagttg cttaatattt ccctcgatat tgtcgtccat
     7321 ccaacgttca acgagccaaa catgaccaaa cagtttcaaa aaatcattcg aaatagttgg
     7381 ataggtgtca gatggttctg caataatgac attgatcata tcatttccat attggtcatc
     7441 gcctatcttc gtcacccgca tgcttttata ctctaaatca tattgatgcg tcatctctgt
     7501 gattgttaaa catctaaata taagactcgt cgatgctgca ttcatcattt ttattttata
     7561 agcataggct tcatcaggat attgaatcgt aatactattt gactttacaa tctcagtact
     7621 tagttttgtg ccatttttat tataaaaaat gatgataaaa tacactgaac cagcaggcgt
     7681 tgcatcaaaa tcaaaatgca atttataatg ctgtcctcta cgcaaaattg gtaaacttgg
     7741 cgcactttta tattttgaaa attgctttaa catcaaccac tcatgaatcg gtaatccaga
     7801 gggcatcaaa ggatttataa aagtcacttc accatttgaa aatgatactt tagagccata
     7861 cataaatgta gtttgtgaaa tataattcca agtaacttta aatgttttgt ttttcagcat
     7921 gttgaactct cccaaacttg tcttccaaaa taatgttgta aaaattaaca aaccaacttg
     7981 caatggtagg tgaatcatca ttatgtcgcc caggaatact gcgattcatc actcttgctt
     8041 ggtgtgctgt caatacaggt aatagctctt gaaatgcatg tggatcataa tcatcatgtt
     8101 gcatatatgc tatggcaaaa acagtttgtg acaatgattt cttttgaaat gtttgccaaa
     8161 atttttgatt taatgcctgt atcgacgctt gagatgtatc accttcatta gacaccagga
     8221 cgtctaatgc tgtaccgaac tcttctggtc taagtaatcg catatgttca gcaatcgttc
     8281 caatattaac aagtggttta ccaacaataa ttgcctgagg atttaactga gcaccataat
     8341 acaaggcacc aaatgaaccc atagataaac cagataatat taattcatgt gatttaaaat
     8401 tcagcttttc taatgtctcg tcaataacat tgataatacc ttgttcatat tcagatgaac
     8461 cgatataaaa actaccacct tcaacacgag gatcgccgat aagtaaaaac ggtgcattca
     8521 tacgtttcat catataatat ccttcgaaac cttccgctgt tcgataacca ctaaaatata
     8581 cgtttagtgg cggtttcata tcaccagggt ggaaataata aataaattcc tgtcgttgac
     8641 tatctacgaa acgactacca ccaagtaaaa attgacccat gtctaatcta gaccatcgtt
     8701 tgtgtatagg tcctaaatgt accgtcccgt tcccacgcgc cttaacagtt acacttatat
     8761 aagcatcaaa tggtttcgca ggtatctcta aaggactgtc taacatatca tcagtcaata
     8821 cgatttgttc aattaatgca ccatcagcgc cagtctgaat caatctaaat gtatattgca
     8881 actcgaccgc accatcaata tcaaattctg gccatatttg aatgacttta tctttatcgt
     8941 aaacgagatt attttgccaa gatgcgatag gtttaaattc tttcccaaat tctccactca
     9001 atgtgagctc tgaattacct tggtaaacga catctccttt aaaattcgga tgcacaagtg
     9061 ctaacttagg agaaacctta tctccatact gtcctgagaa gctaactgcc tctaatttat
     9121 tattacgttc ttcaatattc cggtaatgta atggttgaac aacgtatttt tggacatttt
     9181 cgtcttgttc atattcaact gaccaaaatg attcatcaac atacgtattg tatggttcgc
     9241 ttatcatttg taataaattc gttaatgtct ccgagtatgg tgcttgaata tagataaaat
     9301 caaagcgccc ttctgcttca acaatcgctt caatagcctc tacataacca ctatcaaatt
     9361 caaacaatcc aatatcgaag taatcccaac tcacaccttt tttgtgttga aaaataggtt
     9421 ctaaatcgtc tcctccaatt tgcaaaactc taaatttacg tggcatcatt ttcaccttct
     9481 attaactcat cgagctgatt aataatattc ttagaagcat atgcatctat taattttaaa
     9541 gaataggcgt acgcataatt ccaatttttc aaataaaata aataataatt taacgcatca
     9601 tctaattcat caactgtatt tataatacgg ccattgtcat aatcagagac gtaatctgtt
     9661 tgttgaccat taatttgtgg aatcccagcg ctaattgcac taatttgtaa atacaagtca
     9721 ggttcttttg acatatctat cacaagtcgc aacgtccgca atgcttctac aacatcatgt
     9781 tcagcatgta tcgtcttaac agcaatgatg tcatcttgat cttcaggtgt cattaatgct
     9841 gaaacattaa catccgcatt ctgtttagct tggtattcct catttaccga cgtaatacat
     9901 tcacgaagcc acatcggtat gtcattttga tggcgcgata ataaaattaa acggtaataa
     9961 tcttcctgtg cgatataatc cacaagtcgt tgcatcattt gttgcaaatc agcgtcactc
    10021 ataccatcta tccatacacc tataaatgtt tccatcaatt gactacttat attaggtgat
    10081 tgtctcgttt caaatggtgt gattcgaatc attgtattct cccgctgata cttctcttga
    10141 tgactaatta aatcacgttc tagttgcaca ctatcaacaa ttaaatgggc attctttacg
    10201 atagattgat attcctcatc tgacacagtt tcatttctat ttttaaaaaa tgaataactt
    10261 aatgatttcg ctggaatatg attggctatt tgtcgattgt gcctagcatc tgaagccaca
    10321 atcacatgat catcttcatg tatttgttgt gcaatcattg cttgaaattt ttcttcaatt
    10381 agttgagcca tattgttata ttctgtttgt tgatagtgat gttgatatct ttttgaaaca
    10441 gtgactctgc catttttcaa atcttcatga agtacacaat ctccattaat cgttaaatat
    10501 tcttggtaag aagcctctcc ctgatcatca aaataacgta tcgctgataa ataacctctg
    10561 tcatcaaaaa tataacgccg ttgtaactga tctctttcaa attcttcaaa ccaaattgaa
    10621 tacccttctt gactaaaata aatatttgta taggtctgtt cactcgtcac acattttaat
    10681 aaatacggtg tgtacacaaa ctcaacatca tccggccatt ttaagtgatg ataattaatc
    10741 gcttgtggcg catggtgact gaatccttga atttcatcaa acacagacga atactttgtc
    10801 tcatataagt catatcgatg taaaaatgtt cttaaatttg gtgcatgatt gagaacaatc
    10861 agttgataat ctaagtcatt ttcaaggtgc attcccatta aactaatcat atcgtcaaat
    10921 tccgtcttat tttgtagttg ataatacggc acagtcgtgt cttgccacca tcgttggtca
    10981 tcgtaccaag ctggaataaa gtatttcata attacctcct taccaatact ggtttaaaaa
    11041 tggcttatat ttatcaaaat ataaatatgt acgaattgtt tctgcaatat taatactgat
    11101 gtaaactaat acaatcagtt gtactgagaa ataaatttca gtagataaat gcggtacaaa
    11161 caatgtgaaa taaagcggta taccaataat gactgtaact aatgccaatc caaaccaaca
    11221 tacgcgtcgt gcttgataat ttaaataacg ttctgtatcc ttaccaggtt taactcctga
    11281 aaaataattg ccactcttta agaaatcttt ggatttttgt ttagtattga ttaaaaatct
    11341 cgataaaaaa taacccaata acatttgaat cactaaatat actgaaatac ctactggact
    11401 atcaaatgtc agcattggca tgtcatctga tatgctttta ttaaacatag ataaaataaa
    11461 atgaatgcca ctttttaaga aaacaaaagc tgaaatactc atcattaaag taatactgcc
    11521 tgcagggtta actttccaag ataaataaga tttcatattt gttgcggaaa cgttcattaa
    11581 atcgatatat ggtattctca cttctactaa ttcaataaat aataagataa acaatgtgat
    11641 tatcacaagg atgattaaca acgcaatcac aatatgactt gcatctatat attccatttt
    11701 ttgatgcatc attgatttaa taatactaac cattacaatc ggcattggtc ctgcgatgcc
    11761 gtagcgacta tttttgtcag ctaaccaaac taataacatc gttccagtaa ccaaaatcaa
    11821 tattgttaag taaatattgt cttgatgaac acgttctttc gaaacatatt catgaatcac
    11881 aaaataactt tgaataacac ttaaaattaa tgttaagatg cgctctttat aatgcttctc
    11941 aagacttgtt tgtttcatgt atttatccat attccgataa gaaatcagca tcaagataat
    12001 cattgatgtt aaccacggac ctaatcctaa agtgaaaatg tttaaagtat taacgtctcc
    12061 acccatatta gaaatagcta ttttaaaaaa tgactcatgt tttacttgca tatcgttata
    12121 ggaaacgatg gaaatgtttg tgcctaatat ataaataaac aagataaaac atgtgtatag
    12181 catacgttta tatataattt tatattcgta ttgttgtaaa agttttaaca tgttgcacct
    12241 cttttatatc aaaaacatta aaaagactaa gggttcatca ctaattatta aaatcctata
    12301 tcgatttttc tagtgattgg tgcctcagtc tttttaattt tagccagcta taaattcaat
    12361 ttatgcttga gaatcatctt gatcattttc atctttcttt ttctttctct tcattaaacc
    12421 taaaccaact aataatgtca taacgccacc tagtaatcca ttttgtttta ttgagtcacc
    12481 tgtatctggc aatctttttt cactttgtgc tggtgtgcca ttatgtttag tcacttcaga
    12541 tgttgcactt aatgtagact gagattcact cgtgctcgtt gttgcttcac ttgataagcg
    12601 agatgtgctc gtgctgtgag tatgatgcat actcattgag tctgacggat gcattgagtt
    12661 agattcggat gtacttgttg agccggacat acttgttgat gttgagtcag aaatgctttg
    12721 tgaaccagac atagatgtac tcagtgattc ggatgtgctt gtcgaatcgg atgtgctcaa
    12781 tgacgttgat gtgcttgttg acactgattc tgagtcacta attgatgttg agtcggattt
    12841 gtcttgtgac attgaaacac tcgatgaatt agattcactc attgatgttg agtcagatac
    12901 gctcgttgaa cctgaaccag acgtacttaa tgattcagat atgcttgttg aagttgaacc
    12961 acttgttgag tccgatgtac ttgtcgatgt cgagtctgaa tctgatgtac tcaatgattc
    13021 tgagtcactg atagaagttg aatcacttgt agattctgat tctactgtac tttgtgaacc
    13081 actgatactt attgaagtag aatcactgat actgtctgat gttgataatg atgtcgacac
    13141 cgatgtgctt tgtgatgacg atgtactagc actcattgac attgatgttg atatcgatgt
    13201 acttaaggaa ccagatgcac ttgtacttgt tgactggctt tgtgacattg aatcacttaa
    13261 tgatgtagat gtgcttgttg agctcgagtc acttacactt gttgaacctg atattgagtc
    13321 acttaaactt gtcgatgttg aaactgatac gcttccgctc attgagtcag atgttgaaag
    13381 tgatgtactc gttgaatttg atccactgat gctagacgaa tcacttgtag acattgagtc
    13441 gctttctgat gcactgatgc tcatagagtc aaattgacta ttacttgttg agcttgactg
    13501 cgaatcgctc acacttgttg acgttgattc tgatccactc atactttgcg agctactcaa
    13561 tgattttgaa tcacttaatg aatccgaagt gctaagactt gtggaaccac ttaaagatat
    13621 tgatccactt aatgagtcgg agtcacttgt actagtagaa tcactcattg atattgaatc
    13681 acttagcgag gtagactcgc ttacgctttc tgaaccactt aatgatgttg aggtactcaa
    13741 tgaaccagat gtacttgttg aagtcgaacc acttgttgat tttgaatcac ttaatgaatc
    13801 agattcactc acgctttctg aacttcttag tgacgtcgat acacttaatg atgacgaatc
    13861 gcttgtgctt actgaatcgc tcatcgattg tgagcaactc aatgaacttg actcgcttac
    13921 actttctgat tttcttaatg acgttgagac gctcaatgag ccagaatcac tgacacttgt
    13981 tgagccactc atcgatttag agtcactttc agaattagat tcacttacac tttctgaatc
    14041 atttacagat tctgacatac tttgtgaatc agatatgctt gcgctcatta cttcactagc
    14101 cgatgttgat gtacttgtcg aatcacttaa cgatatagac acactcatcg aaccagatgt
    14161 actcgcactt gttgagtctg atgttgaatc actaacacta tcagataatg acgttgaatc
    14221 actcatactt gttgatgtac ttgtcgaaag cgacatactt tgtgaatcac tagtacttgt
    14281 acgcatcgaa gtactagttg aagctgatgt actacgagag tcacttgttg atgttgatgt
    14341 acttgctgat cctgatgcac ttgtacttct tgatgtgctt tgtgaatcgc ttaatgatgt
    14401 tgatgttgaa tcggattcac ttgtactttc tgatgttgag ccagactctg atgtacttac
    14461 cgatgtagat aaacttgcaa tggtcgacat gcggtttgaa gttgatgtac ttagcgaatc
    14521 acttaatgat gctgatgtgc tttgtgaatc ggattcactg ctttgcattg acgcagatgt
    14581 tgaatcactt aaagatgttg acgacaaatc ggattcactt gcgcttgctg agttactgag
    14641 cgatgttgac gtacttgctg atcctgagtt acttgtactt gttgagtcac tgagtgatgt
    14701 tgacgcactt tctgatgttg actcacttgc acttgttgag atactttgtg aatcagattc
    14761 actctctgaa ccacttaatg acgctgatgt gcttaccgaa cctgattgac tcgtactttt
    14821 agatatactt gttgaatcgc ttagtgacgt tgatgtcgat gtggattcac tttggcttgt
    14881 tgactgactt gtatatgatg atgtgctttg tgaatcggat tcgctcgtgc ttgtacttgt
    14941 tgagtttgag gcactttggc ttgctgagtt tgagtctact ccgctttgat tcattgaggc
    15001 acttagtgac aatgatgtac tcgttgagtc agacaaactt gtactcgttg acgtacttgt
    15061 acttcctgat gttgattgag acatacttat gctcattgat gttgaatcgg atttactttc
    15121 acttgatgat gttgagtcgg attcactttc acttgtagaa ccacttaatg atgtggatgt
    15181 actaatggaa tcagatttac ttgtactgat tgaatcactt gtcgacattg atgtacttaa
    15241 tgaatcagac ttactatcac ttgtggaatc acttaatgat gttgacaaac ttgtagagtc
    15301 agacaaactt gtactcgttg acatactcag tgatgttgaa acactctcgc tcttaaacgt
    15361 tgacgttgat tcactgatac ttgtcgatgt tgaaatggac gtactaccac ttgttgaatt
    15421 acttaatgat gttgatgtgc taccagattc tgatgtactg tctgataatg acgtactctc
    15481 acttgtcgaa ctactcactg actctgatgt tgattcagac gtactttcac ttaatgattc
    15541 acttaaaaag gcagatgcac tttgtgattc tgaatcgcta gtactatttg attcacttaa
    15601 tgatataaac gtgctctctg aagcagatat tgcttcactt atagagtcgc tcgttgacgt
    15661 tgattcactt attgaatcag actctgatgt acttaagctt gtggaatcac tcatagatgt
    15721 tgatgtacgt tctgaattac ttgtacttaa cgatgtcgaa gtacttaccg aaccagatgt
    15781 gctcgtaaaa gcactttgtg atattgattc acttgatgca gttgatgctg atttgctatc
    15841 actcgttgaa tcactaaacg acgttgatat gctcattgaa tcggattgac ttgcactcat
    15901 tgaaccagac gtactttgtg attccgaagt acgtactgaa gcacttgtcg acgttgatgt
    15961 acttgttgaa cctgattgac tagtactttg tgataatgac ttactatcag aatcagatgt
    16021 actttgtgaa tcacttaatg attctgatgt actacctgac tgagacgtgc tcattgaact
    16081 acttacggac attgatttac tgtctgatgc agataatgac ccacttgtac tgatagagtc
    16141 acttactatc tctgaagtac tcatcgagtc tgatgtactt gttgagacac tttgtgatgc
    16201 tgctatgctt agtgatccag aaacagaacc acttgtgctc gtcgaatcgc tcaatgattc
    16261 tgatgtactc atcgattttg aatcacttgt acttaatgat attgatgtac tttgtgaatc
    16321 tgatttgctt gttgacgcac tttgagagtt ggctatgcta tttgaaatac tgatagagtc
    16381 cgaggtgcta gctgactcgc tcaatgatgt tgatgtacta attgcattcg atgtactgtc
    16441 acttaatgat gctgatgtac tagacgaccc tgatatactc gttgataagc tttgtgactt
    16501 agacaagctt cctgatgtac tcatacttaa tgagtcactg agtgatgttg atgtacgcaa
    16561 tgaatcagat gtacttgttg atagactttc ggatttttca gtactgctag agtttgaaat
    16621 agaatcgctt aatgatgttg atttactagc tgaatccgac atgcttgatg atacactttg
    16681 tgaattcact aaacttgtgc ttgttgagct tgatacacta ttactttcag atgtgcttaa
    16741 tgacttagat gcactcacag aatcagatag gcttacactt gtcgatttcg aggtactagc
    16801 tgatgtagat accacaatcg atcctgatgt actcgttgat gcactttgtg agtcagcttt
    16861 acttgttgac acactttgag attgttgtgt acttcctgat gttgatacgg aatcactcat
    16921 gctatttctt gttacttcat atttaaaagt tgtcgtcgtt ttgttaccgc tcgcatctgt
    16981 agaaacgatt gatatagtac ttgtaccaat gtttgttggt gtaccactaa tagtattatt
    17041 tgtactatca aatgttagtc cggatggcaa tccagtcact gtattcgtca ccgcatttcc
    17101 actgttatct tgcgtagcaa ttttaatcgg ggatattggt gaatacactt ctgatgattg
    17161 atctcctatt ggtgtcactg ttggtgctgt cgtatccaca acatttattg taaaagttgt
    17221 cgtcgatttg ttatttgctt ggtcagtaga cacaactgtc actgttgatt gaccaatttt
    17281 tgttggtgtc ccaatgattg aattcgttgc actatcgtaa cttaatccgc ttggtaatcc
    17341 tgtaactgta tttgtcacag tcccagtacc attatccgtt gtagtcaata caataggatt
    17401 cattgtttta cccacttcta tggtttgatt gcctacagtt acagttggtg cttttacatc
    17461 agtaaaataa tatgtcactg attgtccagc attcgtcatt tttacagttt tatttgtatc
    17521 attataagtt gacgcatatg aactatcgac ggacgtgtag ttatatcctt tagcagtcaa
    17581 tgcagattgc tgattatcga ttgtcacgac ttgatcaaca tttcctgaat atgtttttgg
    17641 tggaataata tctttacctg ttgttacatc aacgtatctc acttgtgtaa cagcagactc
    17701 tgtatattcg aatgttccaa attgtacttg ttgtaaattt gtcgcgccac ctgttgaggc
    17761 tgtcattgat aatgaaaagt tggtcgtacc acttttcgca atccaatctg aaatattacg
    17821 tgtccatgtt tgacctgcat atttgacagt cataaccttt gtatcaccat tatagttaat
    17881 atcaaaatct tggaacgtgt tatttgtagg ttgaacattt aacttcgcag cattatcagc
    17941 tgttgaactt gatgtatacg ttgtcgcaac accataacta tctgttgtta caaatgcacc
    18001 aaacgcacct ccaccagcta cattagatgg gtcagcattc gcctttgcag ctgaatttgg
    18061 tttagatgta ttgtgatacg tatccaattt gaagccaaat gcgttactta agccaccaat
    18121 acctactgcg gcaccgttta accctgtttc acctaataca cctggtgaaa aggcaaaacc
    18181 gataccatct ccaccatttc catgcccttc atatttgtta cctaaattta cttttccaga
    18241 aaaatgaaaa ctcttattag agtcaatacg tgttcctaat gtaatagcac ctttttggct
    18301 gtatgcatcc tgtgttaacg tcacaatacc ggtactttga tcataggtag cattacctga
    18361 cgttgtcata tattgtttta agttatcttt attaactgta attgtattag cagttactgc
    18421 ggttgtcgtc gctgctgacg caaatgttga catagctaag cgactgaaag ttcgaagttt
    18481 tactggtgcg gtgctagttg acgttgtgct agttttgttt aagttgaccg aagatggcgt
    18541 tgtgctttgt gaagtgttat ttgatgcagt actttgattt gttgatgtat taattggttg
    18601 ttctgtactt gaagttgaag ctacagattt agtatcagaa cttgatgtag tattctttga
    18661 ggatgttgat tctgatgtag atgtcaattt ctcttgttga ttgcttgtac tattagttgt
    18721 cgaagtgacc ttttcagact tttcacttga gactgtgtca ctatttgatg tttgtaccga
    18781 actactattt ttcgttacac ttgtggaatc ggctgttgat gttgatgctt cgattgtcgt
    18841 tgagttttga ttacctactg tttcactttg tgtgtttaat tcagaagtta atggtgcatc
    18901 agaagccgca aaagcttgct ggtcatgcaa catatttacc gtgaatgcac caccaataac
    18961 cgccgtagtt ttcagtccgt atcccgtcat ttttttacta atgctttgat tatcttgact
    19021 cactaaacta tgactaataa atggtagccc cataattttg aacatttcta tttctttaat
    19081 tccggatttt acccaatttt ttccagattt ataaagtctt actcttgttt tttcgtttgc
    19141 taagctgtca tgaaatgctt tctgtctttt actcatgtaa taactccttg tattatcttt
    19201 acattcatta gattataata tatgccacta ttcaatttaa tacaactctt ttttgataca
    19261 aaaatactca ttttgttaaa atttgtaaaa attcattttt attcgtctaa atgtaatcgt
    19321 tttcatattt ttaaaattac tttttctcgt ttatgcgtat aatctttttt tatataaatt
    19381 tggctaattg gctttatgtt taatcattat aattgtttcg tttttaaaat aattattgta
    19441 ttaatatatc tataccatcc accttttatt tataaatagt taatttacaa ctaaacgata
    19501 aatattatat gcaaaataca tctttaatat taaagtaata ccaatatttt ttcaataaac
    19561 ctagtgtaat atatgtgtaa ttctaaaaga ttcttcttta aaaatataaa taccacgaca
    19621 tattgcttta acattttcat ttataaagcg aaaaaatgca tcgctactaa gttgaatgtt
    19681 tagtaaggat gcatttaatt cactaaaatg attaaattac ttatatcttt tcatctgatt
    19741 gattatcgaa atttcttcct tctaaacctg ctaactcttc tttagaagct gcaggtgctt
    19801 tcatttcaaa tatctcattc actactgtgt aatcgtaata tcctaatctg gcaataggtt
    19861 taatcgactt aatgtccaat ttaccattat caagaataac cttatcgtca atatgaactt
    19921 gggcaactct tcctataaca atatctacgg tagatactgg atctccagtt ggaatacgaa
    19981 tcgtttgaac gtactcacat tcaaaatgaa ctggcgattc ttttacacga tatcctggag
    20041 cttctataca tttttccttt gttacatctg caaaattaaa ttcatcctct tctggtggca
    20101 atgctttcga tgataaatta actgcttctc ttaaatcata cgttgccata ttccacacaa
    20161 accaacctgt ctcttcagca tttttcactg tatctttacg ttcgtgatca ccaagaacgg
    20221 attgatttgc tgcgaacata accataggcg gatcccaagt taagttttga tactgactat
    20281 aaggcgctaa attatctttc ccatctttcg atacagtaga gatccaccct attggacgtg
    20341 gtactgtact acttttaaat gggtcgtgcg gtaaaccatg acttcttaca ccttgttttg
    20401 gcgaataatt catactatct tcacccctta taagtaatta catttaaggt tacgccctct
    20461 ttacataagc gtctaatata aataaacaat ttatttataa gtagaaacta tatatgacgt
    20521 ggttgcttat aatttgcgtt cttgattcga aaaattcaga taaggattta tacaattaat
    20581 atttatgata tcttttgtaa atttaattaa ttatagttac ttcaatcatg attagtttat
    20641 aataataaag tgaaattgaa aaagacagct attatgcgat gagcgaaaaa cttcaagtaa
    20701 aacaagaatt taacggcgtt acaaggcaat acttgaattt cagaacagat agtatcgatg
    20761 atctggataa gttcaaaaaa gaattcaaac ctgttatgca cttaaaaggt gatgcattca
    20821 atcaacagtt acaatctttg attaacaaat atccacaaat acaaaaaaat atgaaatcag
    20881 agttcattgc ttattatgat aaagaaaaaa atagagaaac agtaaaaaac tatgcttgga
    20941 accttcaaaa atctataaat gacattatgc aatcatatcc tagcacaaaa tttgtacagt
    21001 tttataaaga tgatgtttcc ccgtcaatgg tagatggaaa tggccgttta aaatcggata
    21061 ctaatgtaat ttccatcgag ggaggaaagt atgatgagaa caaatgatca tataaccgat
    21121 ttagatgcta ttgagttaag tagcggttgg aattacaatt cgaaattgga gactggcaaa
    21181 aatgcactac tatcagatag ccttttaaga gatattaaag tattaagttc taatgctaaa
    21241 ggcgacgctg acatactaga aacagaactt cgtgctgtaa taaactcaat gtctcaaaac
    21301 gaaggtaaat ctgttgatgc acttaaaaat agtaccgctt ctctaaaaaa caatctaacc
    21361 aaattaaaag agcaactcga caaaactaca cattaatttc atacattttg agtgaacaga
    21421 attatatttc catagcaaac attcctaaac tacacatacg ttacataatt gattcatttt
    21481 tatagaaacg ggtaaaaatg ataaagtaat catctttcac aaaaaatcaa atattgggag
    21541 gaattaaata tgattttgcg ttacgcaacg aatttaaaac ttgcgaaaga gctataccaa
    21601 gccagtaaac ctaaattgca agaagataag ggtatgattg aactgtttga acatactttt
    21661 ggcttgcaaa aagaacttgt aaaatacgtc ggcattgcag aagcaactac tgctgcttta
    21721 tactcagcta gctttatcaa taaaaacatt tctcgccttg catcactatc aacgataggc
    21781 attttaagtg tagctgctta caaacatttc gaagctggcc aaggtaaaaa aggtgcacaa
    21841 catgcattaa atttattagg tcttgctact ttaagtttac tagatacatt ttcttgtaat
    21901 aaaaaatagt cttcatttta gaagcacata ctttagcacc tgtgtacttt tgattcaatt
    21961 cataaacggt ataccgctat agcatgtgtt ttattatttt tctggtctct ttatcgtaaa
    22021 tattccccct aatttagcat aatcatttcc ggctaaacga gaaacagcct gtaatgcgtg
    22081 tggattgatt ttaaaatcac cctcataaat actgtcatca atatgataag ttacaatttc
    22141 acctattatt aaatcagccc catctaatac atctccaagc aatatcattt gcgatagttt
    22201 acattcgaat ctcattttcg catctttaat tcctggcgtc ttaatcgttg tagatgttaa
    22261 aagtgataat tctgtacgac tcaactcact gtcaccatat gctaacggcg ctgcagtctc
    22321 attaatatct tgaacattat cttcgtctgt aatatgcaca acaaagtctc cagtccgttc
    22381 tatatttaat gcagtatctt ttctcttacc tcctgcacgt tgaactgcaa tagcaatcat
    22441 tggcggatga ttattaacaa tattaaaaaa gctaaatggt gctgcattta ctgatgcatc
    22501 ttgatttaat gttgtaacaa aagctatagg tcgtggaata attgaaccaa ttaataattt
    22561 atagttttct ctagcagtta atgattgtgc atcaaacgta tacataatac ctacctcttt
    22621 tctaagtata tctaggtatt tctccgattt tggttaattt aaacatctat tctcctctga
    22681 aaatcacttg tatttattta gcaaatcttt tgaaatatga cacatatgca tatcttctgg
    22741 atatttttct aaatgttgct gatgttcttc agcactttta atgtagttag acagcggtaa
    22801 gacttccact gcaatttgat ctctgtcttt acgtcgttca atgaactgac gcgcttcaat
    22861 taagtggtca tctacacaac tatataaacc cgttcgatac ttttgtccaa tatcatttcc
    22921 ttgttgattc acactgtaag gatcaatgat ttcaaataaa taattcataa tgtctgtaat
    22981 tgttaacata cgatcatcga aatgaagttt gacacattca gcataaccat catacggacc
    23041 gtctaattta gagcttcttc catttgctct tcctgcttct gtatgtataa ttccaggtat
    23101 tgttgcaaaa aatgcttcaa caccccataa acatcctcct gctacataaa caactgccat
    23161 atttacacct catcatcctt ttttatattt ttaacaaggt tataccattt aataccgcca
    23221 tgacatgatt ctgatacacc ttcattacga tacccatatt tttcataaaa tgaaattaat
    23281 gattctcgac atgttaacgt tacaccatgt cgatgatgat tcttagcaag agtttcaaaa
    23341 tagtttagta agcgacctgc aataccctga ccttgataat ttggtgctac aacaagacct
    23401 aacacactaa tatagccacc ttcactatta tttgtggaga catttttaaa taaatcatcg
    23461 ctaatgtaac gctcttttat gactggaccg ttgatgtatc caattacttc atcattcttt
    23521 tcagctacaa taaaagtttc ttgaatttgt tcaattctca atttcaatgc ctctcgtgtt
    23581 gcagcttctt ccggtgaaaa accaatattt tcaagtgcaa taatttgatc taagtcctgc
    23641 atgattactt ttcttaatac cataaaaaca cttccttagc cctatatatc aaaagtaata
    23701 ccttcatctt tatattcatt attttcaaac taccttcact ataaaaaacg agtcttttta
    23761 ttgaaagcca ttcgccttaa tcaattagac aagttgtatt atacgaatta gtaaataatc
    23821 atgattataa ttcattttaa gcatactcaa aaagactggt acatgtatac cagtctaatt
    23881 cgaaaaaata tatttagtta aaaccatttc aaaaacgact tcacttttcc tggcttagtc
    23941 ggtctgtcta aataaacaaa attatcattg attactgaac tggcatttcg aagtaatgct
    24001 tcaatatcat tcgaatattt cttcaattta tgattgtgaa ataattcttg catcaaaaat
    24061 ggtctttggt cacatgaatg tgcatctgaa gctacaaaat gagccaaatt acattctata
    24121 aattgtaatg ataacttttg aatgttttta ccaaatccac caactaaaga actcgatgtt
    24181 aattgactca gtgccccatt tgcaaccaat tcatataata tttccggatt tttggcgata
    24241 cttctatttc tttcaggatg tgcaatgatt ggtatgtaac ctctcgattg tatttcaaaa
    24301 aacaattgtt ttgtataatg tggtacttcg cccgttggaa attcaattaa taaatatttc
    24361 gaacgattaa taccttgaat actaccatta tctaagcctt tcagaatcga atctgtaatt
    24421 ctaatttctt gcccgggaaa taatttaata tccaatgctt gaacttctgg atgcgttctt
    24481 aactccgcca atttcacaag cacttgttga aatgtattat catatctcgg atgcaaatga
    24541 tgaggtgtcg ctacaatact tgttacacct tcatccttag cttgctttaa tagtgcaata
    24601 ctcttttcaa ttgttttagg accatcatct atatcaacta atatatggtt atgaatatca
    24661 atcatgattc atcagtccca taatatgcat agtaactagc acttttatct ttaggcattc
    24721 tatttaagac tacacctaat aatttagcac ctgttgcttc aataagttct tttccttttt
    24781 taacttcatc tctattatta ttttccgaat taactacgta gacaacattg ccggtaaact
    24841 ttgaaaataa ttgcgcatct gtaactgtgt tcactggtgg cgtatcgata attacaaagt
    24901 tataattcat caataatgtg tcatacaaat ttgcaaatgc ccttgatgta attaactctg
    24961 acggattcgg tgggattggc ccagacgtca agacgtctaa atcttgaatt tcagttgaga
    25021 taatactgtc ttgataagtt gaccaattta gcaataaact tgataggcct tcattgtttg
    25081 gcaaattaaa aatataatgc tgcgtaggtt tacgcatatc cccgtctacg attagtgttt
    25141 tataacctgc ttgcgcatat gcaactgcta aatttgctgc aattgtagac ttacctgcgc
    25201 ctggtgcctc tgaagtgatt acaatgcttt gaactgcact gtcaggattt gcaaacataa
    25261 tatttgatct tatgcctcga aatttctcgc taataggtga ctttggttgt tcatggacaa
    25321 ttaaacttga tgtacttctt cgtgtattcg tcatggtaat tcctcgtaaa ttaaaatttt
    25381 tgtattgaac ctaaaatagg taatcctagt tgcgattcaa catcttcttc tgtcttaata
    25441 cgcttatcta ataattcttt taagaaaata atcaatattg ctaaaacaat accaacaata
    25501 atgctgataa ctaagttgac agatactatt ggagatactt ttacagcatt atcatgtgct
    25561 gaggaaagta tcgtaacatt atcaacactc ataattttag gcatgtcatg agcaaaaact
    25621 ttagatattt tattaacaat tttgtcagat tcagatttat tcccagtggt aactgataca
    25681 gtaataattt gagagtttgt ttgattggtt acttttaaaa atgaattcaa ctcagctgtt
    25741 gaatactgac catcaaattc tctagatact ttatctagaa ttctaggact tttgataatt
    25801 tccgtatatg tattaacaga ctgcaaacta ctttgaacat tttggaaagc taaatcactt
    25861 gaggactttt tcatgttcac taatatttga gtagaagcag tatatttgtc aggcataaca
    25921 aaaaaggtta atgccgcact tactacaaga catattgccg gtaaaataag caataattta
    25981 atattcttct ttagaatatt taatagtttt actaaatcaa acttttcttt catggtttcc
    26041 tccacataat caatcattgt attcattatg tatgttttat aaatcggaca attatatcta
    26101 gtttaacgac cacaaaacat acacaactac attttctcta attatttata taaatatttt
    26161 atcgtt
//
LOCUS       DAKTIO010000017        23658 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.017, whole genome shotgun sequence.
ACCESSION   DAKTIO010000017 DAKTIO010000000
VERSION     DAKTIO010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 23658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23658
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.017"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..375
                     /locus_tag="O0293_000559"
     CDS             <1..375
                     /locus_tag="O0293_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCY0279458.1"
                     /translation="DKNLERNVFLKGYTTTPQKCLEDFKLVVSTSQYEGQGLSMIEAM
                     ISKRPVVAFDIKYGPSDFIEDNKNGYLIENHNINDMADKILQLVNNDVLAAEFGSKAR
                     ENIIEKYSTESILEKWLNLFNS"
     gene            complement(464..820)
                     /locus_tag="O0293_000560"
     CDS             complement(464..820)
                     /locus_tag="O0293_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCY0279459.1"
                     /translation="MKLGMLLIRWMLATSFLVHGTLKFVDLDGTIHFLGSLGLPPTIA
                     VLMSIGEVVGGLALIIGFLTPYVNVFYICVMLGSIFTLKLTRGYVSGYEFEIIHIVMN
                     LACISSYSWKKWLQFY"
     gene            1297..2256
                     /locus_tag="O0293_000561"
     CDS             1297..2256
                     /locus_tag="O0293_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0279460.1"
                     /translation="MNRNIVKLVVFMLILVVAVAGCGQKDTEEKTEMTTIKDELGTEK
                     IKKNPKRVVVLEYSFADYLAALDMKPVGIADDGSTKNITKSVRDKIGAYESVGSRPQP
                     NMEVISKLKPDLIIADVSRHKKIKSELSKIAPTIMLVSGTGDYNANIEAFKTVAKAVG
                     KEKEGEKRLEKHDKILAEIRKKIEQSTLKSAFAFGISRAGMFINNEDTFMGQFLIKMG
                     IQPEVTKDKTTHVGERKGGPYIYLNNEELANINPKVMILATDGKTDKNRTKFIDPAVW
                     KSLKAVKDNKVYDVDRNKWLKSRGIIASESMAEDLEKIAEKAK"
     gene            complement(2302..2433)
                     /locus_tag="O0293_000562"
     CDS             complement(2302..2433)
                     /locus_tag="O0293_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279461.1"
                     /translation="MRYVTMRQFIKRTVKTILVGYVIKFIRNKLSGKSSHPTDNKHN"
     gene            complement(2497..2712)
                     /locus_tag="O0293_000563"
     CDS             complement(2497..2712)
                     /locus_tag="O0293_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HCY0279462.1"
                     /translation="MKVNKAIYVIVALFLGGLGIHKFYADKFGQGLFHLLFFWTGIPT
                     IISIIHAIFVIFTKKADKDGYIIFPKN"
     gene            2877..3428
                     /locus_tag="O0293_000564"
     CDS             2877..3428
                     /locus_tag="O0293_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0279463.1"
                     /translation="MIRQARPEDRFDIAKLVYMVWDDMELELVKHLPKDMVLDAIEKS
                     CVDATYRTFYQHILVYEVENKVAGCIISYSGENELKYEKAWELLDLPEKIKQYGTPLP
                     VKEAKDDEYYIETIATFAAYRGRGIATKLLTSLLESNTHVKWSLNCDINNEAALKLYK
                     KVGFISDGQIELYKHMYHHLIVK"
     gene            complement(3480..4418)
                     /locus_tag="O0293_000565"
     CDS             complement(3480..4418)
                     /locus_tag="O0293_000565"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCY0279464.1"
                     /translation="MSNQYQQYSTVKKYWHLMRPHTLTASVVPVLVGTAASKIYFLGS
                     EDHIKISLFIAMLLACLLIQAATNMFNEYYDYKKGLDDHESVGIGGAIVRNGMSPELV
                     LRLAIAFYILAAILGLFLAANSSFWLLPVGLVCMAVGYLYTGGPFPISWTPFGELFSG
                     VFMGMFIIVIAFFIQTGNIQSYVIWLSVPIVITIGLINMANNIRDRVKDKASGRKTLP
                     ILLGKNASLTFMAIMYFIAYAFIVLTIIIKPGGSLFYLLALLSFPMPVKVIRRFKKND
                     TPPTMMPAMAAAGKTNTFFGLLYALGIYISALFAGI"
     gene            4600..5961
                     /locus_tag="O0293_000566"
     CDS             4600..5961
                     /locus_tag="O0293_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCY0279465.1"
                     /translation="MATGVLEDDIVKEIYGSSKEWVSVEVKLSQSLDPSTLFHLTDNE
                     AGDRFYMRLNDNRTSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHL
                     RVVGGFQFSSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEALNDL
                     MDLFNNISDIDVDEQIGGITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFD
                     KDISIPYILKQAYSKEKNSYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKR
                     SQDEDEDTKNVEAFLKDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYH
                     LYTEIKAPLKDDSYISLIDNLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYID
                     IYDDCEFIVAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMN
                     GKS"
     gene            5948..7621
                     /gene="menD"
                     /locus_tag="O0293_000567"
     CDS             5948..7621
                     /gene="menD"
                     /locus_tag="O0293_000567"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCY0279466.1"
                     /translation="MGNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEA
                     HPNIKTWIHPDERSAAFFAVGLIKGSERPVAILCTSGTAAANYTPAIAESQISRIPLI
                     VLTSDRPHELRSVGAPQAINQVNMFNNYVSYEFDMPIADDSKETIDAIYYQMQIASQY
                     LYGPHKGPIHFNLPFRDPLTPDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGL
                     IIVGDMQHQEVDQILTYSTIYDLPILADPLSHLRKFDHPNVICTYDLLFRSGLDLNVD
                     FVIRVGKPVISKKLNQWLKKTDAFQILVQNNDKIDVFPIAPDISYEISANDFFRSLME
                     DTTVNRVSWLEKWQCLEKKGRKEIKCYLEQATDESAFVGELIKKTSEKDALFISNSMP
                     IRDVDNLLLNKNIDVYANRGANGIDGIVSTALGMAVHKRITLLIGDLSFYHDMNGLLM
                     SKLNNIQMNIVLLNNDGGGIFSYLPQKESATDYFERLFGTPTGLDFEYTAKLYQFDFK
                     RFNSVSEFKNATLLSETSTIYELITNREDNFKQHQILYQKLSEMIHDTL"
     gene            7608..8411
                     /gene="menH"
                     /locus_tag="O0293_000568"
     CDS             7608..8411
                     /gene="menH"
                     /locus_tag="O0293_000568"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCY0279467.1"
                     /translation="MTHYKFYEANVETNQVLVFLHGFLSDSRTYHNHIEKFTDNYHVI
                     TIDLPGHGEDQSSMDETWNFDYITTLLDRILDKYKDKSITLFGYSMGGRVALYYAING
                     HIPISNLILESTSPGIKEEANQLERRLVDDARAKVLDIAGIELFVNDWEKLPLFQSQL
                     ELPVEIQHQIRQQRLSQSPHKMAKALRDYGTGQMPNLWPRLKEIKVPTLILAGEYDEK
                     FVQIAKKMANLIPNSKCKLISATGHTIHVEDSDEFDTMILGFLKEEQND"
     gene            8404..9225
                     /gene="menB"
                     /locus_tag="O0293_000569"
     CDS             8404..9225
                     /gene="menB"
                     /locus_tag="O0293_000569"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCY0279468.1"
                     /translation="MTNRQWETLREYDEIKYEFYEGIAKVTINRPEVRNAFTPKTVAE
                     MIDAFSRARDDQNVSVIVLTGEGDLAFCSGGDQKKRGHGGYVGEDQIPRLNVLDLQRL
                     IRIIPKPVIAMVKGYAVGGGNVLNVVCDLTIAADNAIFGQTGPKVGSFDAGYGSGYLA
                     RIVGHKKAREIWYLCRQYNAQEALDMGLVNTVVPLEKVEDETVQWCKEIMKHSPTALR
                     FLKAAMNADTDGLAGLQQMAGDATLLYYTTDEAKEGRDAFKEKRDPDFDQFPKFP"
     gene            complement(9464..9793)
                     /gene="sspC"
                     /locus_tag="O0293_000570"
     CDS             complement(9464..9793)
                     /gene="sspC"
                     /locus_tag="O0293_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin B"
                     /protein_id="HCY0279469.1"
                     /translation="MYQLQFINLVYDTTKLTHLEQTNINLFIGNWSNHQLQKSICIRH
                     GDDTSHNQYHILFIDTAHQRIKFSSIDNEEIIYILDYDDTQHILMQTSSKQGIGTSRP
                     IVYERLV"
     gene            complement(9831..11012)
                     /gene="sspB"
                     /locus_tag="O0293_000571"
     CDS             complement(9831..11012)
                     /gene="sspB"
                     /locus_tag="O0293_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain B"
                     /protein_id="HCY0279470.1"
                     /translation="MNSSCKSRVFNIISIIMVSMLILSLGAFANNNKAKADSHSKQLE
                     INVKSDKVPQKVKDLAQQQFAGYAKALDKQSNAKTGKYELGEAFKIYKFNGEEDNSYY
                     YPVIKDGKIVYTLTLSPKNKDDLNKSKEDMNYSVKISNFIAKDLDQIKDKNSNITVLT
                     DEKGFYFEEDGKVRLVKATPLPGNVKEKESAKTVSAKLKQELKNTVTPTKVEENEAIQ
                     EDQVQYENTLKNFKIREQQFDNSWCAGFSMAALLNATKNTDTYNAHDIMRTLYPEVSE
                     QDLPNCATFPNQMIEYGKSQGRDIHYQEGVPSYEQVDQLTKDNVGIMILAQSVSQNPN
                     DPHLGHALAVVGNAKINDQEKLIYWNPWDTELSIQDADSSLLHLSFNRDYNWYGSMIG
                     Y"
     gene            complement(11094..12104)
                     /gene="sspA"
                     /locus_tag="O0293_000572"
     CDS             complement(11094..12104)
                     /gene="sspA"
                     /locus_tag="O0293_000572"
                     /EC_number="3.4.21.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-specific serine endopeptidase SspA"
                     /protein_id="HCY0279471.1"
                     /translation="MKGKFLKVSSLFVATLTTATLVSSPAANALSSKAMDNHPQQTQS
                     SKQQTPKIQKGGNLKPLEQREHANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTF
                     IASGVVVGKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEG
                     DLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKI
                     TYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNEFNGAVFINENVRNFLKQ
                     NIEDIHFANDDQPNNPDNPDNPNNPDNPNNPDEPNNPDNPNNPDNPDNGDNNNSDNPD
                     AA"
     gene            complement(12632..13786)
                     /locus_tag="O0293_000573"
     CDS             complement(12632..13786)
                     /locus_tag="O0293_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCY0279472.1"
                     /translation="MKLSLNSNSKYLRAPSIRQFSNRMNNLDDCVNLTIGQPDFPMPD
                     VVKKAYIDAINNDKTSYSHNKGLLETREAISQYFKNRYHFSYDPEEIIVTNGASEAID
                     TTLRSIIEPGDEIIIPGPIYAGYIPLIEVLGGKPIYIDTTATQFKITPDALESHISPK
                     TKAVLLNYPTNPTGVVLKRNEVLNIVNVLKKYPLFIISDEIYAENTFSGKHVSFAEFE
                     DIRDQLILIGGLSKSHSATGIRIGFLLGPQYLIDKLTFMHAYNCICANVPAQIACITA
                     LNEGLEAPKYMNEAYVERRNYLVSELTKLGFEITAQPEGAFYIFPSIKHITDDDFEFC
                     VDLLESTHLAIVPGSSFTEFGKGFVRISYAYEMDVLKEGMKRLAKYLNTK"
     gene            complement(13982..14992)
                     /locus_tag="O0293_000574"
     CDS             complement(13982..14992)
                     /locus_tag="O0293_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCY0279473.1"
                     /translation="MTSLKERDYFFDNARAILILLVVFGHMLQPYTSGDKYLSALYLV
                     IYSFHMPTFLFISGYFAKNIDKPYYLEKISKRLIVPYMIFFAFFSIYYFLTGKSDELQ
                     LDPFNPVFALWFLITLFFFHVILVIVRRFNPYKVLSVSIIISIGAGFSDNIDSYLSIS
                     RTIVFFPIFYLGYIFTKKHTAIFKNKKLIPVSIITFILFFIVYVIHPINADWLLGSSP
                     YTSLENEGQSIFSPFKRLILYGIILIAMTAFLNLMSTKKKLYTYIGSRTLYVYLLHGL
                     IIGIVRGFEWYPFDNPISLMTYLYLISISVIIVYVLSTNFVCKWTNPIINLQRPSQFK
                     DS"
     gene            15145..15564
                     /locus_tag="O0293_000575"
     CDS             15145..15564
                     /locus_tag="O0293_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0279474.1"
                     /translation="MYKQLEKLITLTNNDLNLVNRRFGQRTDIKSEQLELLRILFNYD
                     RLSQYDLTMKISREQSIVSRWIKKLVLKGYITSQQSSEDLRCKELILTDQARTLISQI
                     NNARCELIEARCQCLSEVELDNLNQLLDKLNQRRISL"
     gene            15591..15701
                     /locus_tag="O0293_000576"
     CDS             15591..15701
                     /locus_tag="O0293_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279475.1"
                     /translation="MSLCTQQKLNHELQAKVAVIVKIDGKQSPLFLLVYV"
     gene            complement(15771..19541)
                     /locus_tag="O0293_000577"
     CDS             complement(15771..19541)
                     /locus_tag="O0293_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCY0279476.1"
                     /translation="MAKKFNYKLPSMVALTLVGSAVTAHQVQAAETTQDQTTNKNVLD
                     SNKVKATTEQAKAEVKNPTQNISGTQVYQDPAIVQPKTANNKTGNAQVSQKVDTAQVN
                     GDTRANQSATTNNTQPVAKSTSTTAPKTNTNVTNAGYSLVDDEDDNSENQINPELIKS
                     AAKPAALETQYKTAAPKAATTSAPKAKTEATPKVTTFSASAQPRSVAATPKTSLPKYK
                     PQVNSSINDYIRKNNLKAPKIEEDYTSYFPKYAYRNGVGRPEGIVVHDTANDRSTING
                     EISYMKNNYQNAFVHAFVDGDRIIETAPTDYLSWGVGAVGNPRFINVEIVHTHDYASF
                     ARSMNNYADYAATQLQYYGLKPDSAEYDGNGTVWTHYAVSKYLGGTDHADPHGYLRSH
                     NYSYDQLYDLINEKYLIKMGKVAPWGTQSTTTPTTPSKPTTPSKPSTGKLTVAANNGV
                     AQIKPTNSGLYTTVYDKTGKATNEVQKTFAVSKTATLGNQKFYLVQDYNSGNKFGWVK
                     EGDVVYNTAKSPVNVNQSYSIKPGTKLYTVPWGTSKQVAGSVSGSGNQTFKASKQQQI
                     DKSIYLYGSVNGKSGWVSKAYLVDTAKPTPTPTPKPSTPTTNNKLTVSSLNGVAQINA
                     KNNGLFTTVYDKTGKPTKEVQKTFAVTKEASLGGNKFYLVKDYNSPTLIGWVKQGDVI
                     YNNAKSPVNVMQTYTVKPGTKLYSVPWGTYKQEAGAVSGTGNQTFKATKQQQIDKSIY
                     LFGTVNGKSGWVSKAYLAVPAAPKKAVAQPKTAVKAYTVTKPQTTQTVSKIAQVKPNN
                     TGIRASVYEKTAKNGAKYADRTFYVTKERAHGNETYVLLNNTSHNIPLGWFNVKDLNV
                     QNLGKEVKTTQKYTVNKSNNGLSMVPWGTKNQVILTGNNIAQGTFNATKQVSVGKDVY
                     LYGTINNRTGWVNAKDLTAPTAVKPTTSAAKDYNYTYVIKNGNGYYYVTPNSDTAKYS
                     LKAFNEQPFAVVKEQVINGQTWYYGKLSNGKLAWIKSTDLAKELIKYNQTGMTLNQVA
                     QIQAGLQYKPQVQRVPGKWTDANFNDVKHAMDTKRLAQDPALKYQFLRLDQPQNISID
                     KINQFLKGKGVLENQGAAFNKAAQMYGINEVYLISHALLETGNGTSQLAKGADVVNNK
                     VVTNSNTKYHNVFGIAAYDNDPLREGIKYAKQAGWDTVSKAIVGGAKFIGNSYVKAGQ
                     NTLYKMRWNPAHPGTHQYATDVDWANINAKIIKGYYDKIGEVGKYFDIPQYK"
     gene            complement(19769..20203)
                     /locus_tag="O0293_000578"
     CDS             complement(19769..20203)
                     /locus_tag="O0293_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0279477.1"
                     /translation="MFSKVNNQKMLEDCFYIRKKVFVEEQGVPEESEIDEYESESIHL
                     IGYDNGQPVATARIRPINETTVKIERVAVMKSHRGQGMGRMLMQAVESLAKDEGFYVA
                     TMNAQCHAIPFYESLNFKMRGNIFLEEGIEHIEMTKKLTSLN"
     gene            complement(20358..20828)
                     /locus_tag="O0293_000579"
     CDS             complement(20358..20828)
                     /locus_tag="O0293_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499548.1"
                     /note="Essential protein for resistance to osmotic stress
                     from salt; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic stress response protein"
                     /protein_id="HCY0279478.1"
                     /translation="MSRKTYEKIANINGMFNMLEQQIIHSQDMAHFRSEFFYVNHEHR
                     ENYEALLIYYKNSIDNPIVDGACYILALPEIFNSVDVFESELPFSWVYDENGITETMK
                     SLSIPLQYLVAAALEVTDVNIFKPSGFTMGMNNWNIAQMRIFWQYTAIIRKEAL"
     gene            complement(20876..22093)
                     /locus_tag="O0293_000580"
     CDS             complement(20876..22093)
                     /locus_tag="O0293_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase"
                     /protein_id="HCY0279479.1"
                     /translation="MNKFLKYFLILLALVLIVVPIVFATLLFKTSQDAFESSQDSKNA
                     NRQSNLRDNKVNPEEQPISILFLGIDDNDGRRKKGQDAEHSRSDAMILTTFNQSKHQI
                     RMLSIPRDTISYIPKVGYYDKITHAHAYGGPIAAMDSVEATMNVPVDYYVRVNMKAFV
                     EAVNELGGIYYDVPYDLNEPNTDDTGKIKIKKGYQKLNGDEALAVARTRHHDSDLKRG
                     QRQMELIKILFQKAQEVDSIDKLDNVIQIVGKNAKHNLTNSEIKALAKMYLTNDVEIK
                     TAQLKGKDDMLNGIYYYHPSVESIQKYANLLRKDLELSPINDKNDFLDQRVINHYGSL
                     IPLTPLDNSLLRKEQNDTTDKDKTSNENSDSTNNSDSSNQQQPATDQNSNQNQGGTQQ
                     APQASNNQNGVVN"
     gene            22544..>23658
                     /gene="fmtA"
                     /locus_tag="O0293_000581"
     CDS             22544..>23658
                     /gene="fmtA"
                     /locus_tag="O0293_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala esterase FmtA"
                     /protein_id="HCY0279480.1"
                     /translation="MKFNKVKLVIHACVLLFIIISIALIFHRLQTKTHSIDPIHKETK
                     LSDNEKYLVDRNKEKVAPSKLKEVYNSKDPKYKKIDKYLQSSLFNGSVAIYENGKLKM
                     SKGYGYQDFEKGIKNTPNTMFLIGSAQKFSTGLLLKQLEEEHKININDPVSKYLPWFK
                     TSKPIPLKDLMLHQSGLYKYKSSKDYKNLDQAVKAIQKRGIDPKKYKKHMYNDGNYLV
                     LAKVIEEVTGKSYAENYYTKIGDPLKLQHTAFYDEQPFKKYLAKGYAYNSTGLSFLRP
                     NILDQYYGAGNLYMTPTDMGKLITQIQQYKLFSPKITNPLLHEFGTKKYPDEYRYGFY
                     AKPTLNRLNGGFFGQVFTVYYNDKYVVVLALNVKGNN"
BASE COUNT         8033 a         3461 c         4051 g         8113 t
ORIGIN      
        1 gataaaaatt tagaaagaaa tgtttttctt aaaggatata caacaactcc acaaaaatgc
       61 ttggaagatt ttaaattagt cgtttctaca tctcaatatg aaggtcaagg gttaagtatg
      121 atagaagcaa tgatttctaa aaggcctgtt gttgcctttg acatcaaata cggaccaagt
      181 gattttatag aagataataa aaatggttat ttaatagaaa accataatat taacgacatg
      241 gctgataaaa tacttcagct tgttaataat gatgtattag cagcggagtt tggttcgaaa
      301 gcgagagaaa acattataga aaaatattca acggaatcaa tattagaaaa atggttaaat
      361 cttttcaata gctaaatgaa aaaatcctag gtattcatga gtacctagga tttttacgca
      421 tgataaatac aaaaaaagag aggatagtcc tctcaaacaa tagttaataa aattgtagcc
      481 atttcttcca gctgtaacta ctaatacaag ctaagttcat tacgatatgt atgatttcaa
      541 attcataacc acttacatat cctcttgtaa gtttaagtgt gaagattgag cctaacataa
      601 cacaaatata aaaaacatta acataaggcg ttaaaaagcc gataataagt gctaaacctc
      661 caacaacttc accaattgac atcaatactg cgatggtagg tggtaaacct aaagagccta
      721 agaagtgaat tgttccatct aaatcaacaa atttcaaagt accatgcact aagaatgatg
      781 ttgctaacat ccatctaatt aaaagcatac ctaatttcat catgtcacct ccaacattca
      841 tatttttaaa acatagtcca aattatattt gtaaacatgt ggaaagtaaa gattttttat
      901 aagattaatt attaaatatt ctattgtatt gaacaattgt tgtagaaaga tgtttaagtc
      961 atcagttact ttttgtattt ataaataaag attagtacag catagtgcaa agattatagg
     1021 gaaatgaatt aactattgca tcgctattta tgaatcgtaa acactattat gaaattgcga
     1081 gagtagtatg gacaattaga atgatggttt gagataaatg agtgttatta accagtgcgt
     1141 cattttcaaa attagatgcc atttgctgtt tataataaga agatttgata attaactatg
     1201 ataagaaaaa ataataatcc cctaaataca ttataaacaa attaaatgat aaaataataa
     1261 tatatatttc tactaaagtt gtttggaggt aaatgtgtga ataggaatat cgttaaacta
     1321 gttgtgttca tgctaatttt agttgtagca gtagcgggtt gtggtcaaaa agatactgaa
     1381 gagaaaactg aaatgacgac aataaaagat gaattaggaa ctgaaaaaat taagaaaaat
     1441 cctaaacgtg ttgttgtatt agaatatagt tttgctgatt atttagcagc attagatatg
     1501 aaacctgttg gtattgcaga tgatggcagc actaaaaata taacaaagtc agtaagagat
     1561 aagattgggg catatgaatc ggttggatct agaccgcaac cgaatatgga agtgataagt
     1621 aaattaaaac cggatttgat cattgcagat gttagcagac ataagaaaat caaatcagaa
     1681 ttgagcaaaa ttgctccgac aatcatgtta gttagcggta cgggagatta taatgcaaat
     1741 attgaagcat ttaaaacagt cgctaaagca gtaggcaaag agaaagaagg cgagaagcgt
     1801 ctggaaaagc atgataaaat attagcggag attagaaaga aaattgaaca gagtacgtta
     1861 aaatctgcat ttgcattcgg tatctcaaga gcaggtatgt ttattaataa tgaagataca
     1921 tttatgggac aattcttaat taaaatgggt attcaacctg aagtcacaaa agacaaaact
     1981 acgcatgttg gtgaacgcaa gggtggtcct tatatatatt taaataatga agaacttgcc
     2041 aatatcaatc caaaagttat gattttagcc actgacggaa aaacggacaa aaatagaacg
     2101 aaattcattg atcctgcagt ttggaaatca ttaaaagctg tgaaagataa caaagtttat
     2161 gacgttgacc gaaataagtg gttgaaatca agggggatta tcgcaagtga aagtatggca
     2221 gaagatttag aaaaaattgc agaaaaagca aaataaaaat acagcgctac tcgtaaatca
     2281 tataagagtg gcgctgtatt tttaattatg tttattatct gtcggatgtg atgatttacc
     2341 tgaaagttta tttcgaataa atttaattac ataaccgaca aggattgttt taacagttct
     2401 tttaatgaat tggcgcatcg ttacatacct catttctcta tatcttacga actatatacc
     2461 cattcatata tgctttttaa acgtcattgt cacaatttaa tttttaggga atataatata
     2521 accatcttta tctgcttttt tagtaaaaat gacaaaaatt gcatgtatta ttgagatgat
     2581 ggtagggata cctgtccaga aaaataataa gtgaaaaaga ccttgtccaa atttatcagc
     2641 ataaaattta tgaataccta aacctccaag aaataatgca acaataacat aaatggcttt
     2701 attgactttc atttgtaaat cctccttaac tataattcta cttaaattcg ttgtgaaaac
     2761 caatatttct aactttagaa ttttcaaact ttctaaaatt ataagtatat ctttttaaaa
     2821 taagctagaa tttctatata ataaatgtta ataacgtaaa agggaatgat gacatagtga
     2881 tacgtcaagc acgtccagag gaccgatttg atattgcgaa gttagtttat atggtttggg
     2941 atgatatgga attagaattg gtaaagcatc tacctaaaga catggtatta gatgcaattg
     3001 aaaaaagctg tgttgatgca acatatcgaa ctttttatca gcatatttta gtttatgaag
     3061 tagaaaataa agtagcaggt tgtattatta gctatagtgg tgaaaatgaa ttgaaatacg
     3121 aaaaagcatg ggaactactt gacttgccag aaaaaataaa acaatatggc acgccattac
     3181 ctgtaaaaga agctaaagac gatgagtatt atatagaaac aattgcgaca tttgcagcat
     3241 atagaggtag aggcatcgcg acaaagttat taacgtcatt acttgaatca aatacacatg
     3301 ttaaatggag tttgaattgc gatattaata atgaagcagc attaaagtta tataaaaaag
     3361 taggctttat atctgatgga cagattgaat tatacaagca catgtatcat catttaattg
     3421 ttaaataaaa aaactcgaca gttcgatgta agtcgattgc cgagtagtat cattttctat
     3481 taaatgcctg caaataatgc actaatataa atacctaatg catataataa accgaaaaat
     3541 gtatttgttt taccagcagc agccattgct ggcatcattg taggcggtgt atcattcttc
     3601 ttgaaacgtc tgataacttt aacaggcatt gggaatgata acaacgcaag taagtaaaat
     3661 aatgagccac caggtttaat aatgatcgta agtacaataa aggcataagc gataaagtac
     3721 atgattgcca taaatgttaa agaagcattt ttacctaata gaatgggtaa agttttgcga
     3781 ccacttgctt tatctttgac acggtcgcga atattgttag ccatattaat taaaccgata
     3841 gtgattacta taggtacact taaccaaatt acataacttt gaatattgcc agtttgaata
     3901 aagaatgcaa taacgataat aaacataccc ataaatacgc ctgagaataa ttcaccgaaa
     3961 ggcgtccatg aaatagggaa agggccacct gtatataggt aaccaacagc catacatact
     4021 aatccaactg gtaataacca aaatgaagag ttagcagcta aaaacaaacc taatattgct
     4081 gctaagatgt aaaatgcaat ggctaatcgt agcacaagct ctgggctcat accgttgcga
     4141 acaatggcac caccaatgcc tacagattca tgatcatcaa ggcctttttt ataatcatag
     4201 tattcattaa acatattagt tgctgcttga ataagtaagc atgctagtaa catggcaatg
     4261 aataggctga ttttaatatg atcttcgcta ccaagaaaat atattttaga tgctgctgta
     4321 ccaactaaaa cgggtactac ggaagcagtt aatgtatgag gacgcattaa atgccaatat
     4381 ttcttaactg tagaatattg ctgatattga ttactcattg aatacaaaac ctctttaaat
     4441 catatttttt ataacaaaga tattttaata gaaagtagca taaaggtcaa ttgacgtgaa
     4501 aaattgcaag ataagtttaa gcaattcttg taacacaatt atccaaaaat gatacaatat
     4561 aataatgagt gaaaaaagac gaagaaagaa gtgaaatgga tggctacggg cgtattagag
     4621 gacgatattg tcaaagagat atatggcagc tcaaaggaat gggtttcagt tgaagtgaaa
     4681 ttatcacagt cactagaccc gagcacatta tttcatctca ctgacaatga ggcaggagat
     4741 cgcttttata tgcgtttgaa tgataatcga acgtcatatt ttggctacaa agcaattcaa
     4801 ttattcaaaa ataattctaa aaataaacaa tctattttta aagactggga aaaattaaaa
     4861 cataacatca catttataca tccgcaatct gagaaacatc atcttcgagt tgttggaggg
     4921 ttccaatttt caagtcataa atcagatgat gaatggcgag agtttggact aaatcatttt
     4981 gtattacctg aagttttaat ttcaactgat aataatggga catttttaac ttatacagtt
     5041 aaaagggaaa gttttactgt tgaggcattg aacgatttaa tggatttgtt caacaatata
     5101 tcggacatag atgtggacga gcaaattggg ggaattacta gaaatgaaga tatttataaa
     5161 gatgactggc gtcaacttgt agtagaagct atagaatcta ttaataatga agaaaaaatt
     5221 gtactagcac gtagacggtt aataaagttc gataaagata tcagtattcc atatattcta
     5281 aagcaagcat attctaaaga aaaaaacagt tatatattct tgttagaatc acaagattct
     5341 atattctttt cacaaacacc tgaacaatta ataaaggtca ataataaaat actatcgact
     5401 aaagctgtag caggtacaat taaacgttca caagatgagg acgaagatac aaaaaatgtt
     5461 gaagcatttt taaaagataa taaaaactta atcgaacatc gatttgttgt tgacagtatt
     5521 ttacatgata ttaaacctta tatcactgaa ttacattatg ataagacgcc taaaattcta
     5581 aaaaatgatc atttatatca cttgtacact gaaataaagg cgccactgaa ggatgattcg
     5641 tatattagtt taattgataa tttacatcca acacctgctt taggtggcta tccaaaagaa
     5701 tttgcaatgg attttattga gcagaaagaa tttggtacgc gaggattata tggtgcaccg
     5761 gttggctata tagatatata cgatgattgt gaatttattg ttgcaattcg ttcgatgctt
     5821 attaagaaag cacaagcaac tttatttgct gggtgtggca ttgttaaaga ttctgatcca
     5881 gatagtgaat tggcagaaac gaaccttaag ttcacaccta tgatgaatgc attaggagtc
     5941 gatatgaatg ggaaatcata aagcagcttt aacgaagcaa gtttttacat ttgcatctga
     6001 gttatatgcg tacggcgtaa gggaagtagt tatcagtccg ggatcacgct caacgccact
     6061 tgcacttgca tttgaagcac atccaaatat taaaacatgg atacaccccg atgagcgaag
     6121 tgcagcgttt tttgcagttg ggttaattaa aggcagtgaa agacctgtcg ctatattatg
     6181 tacgtcaggt acagcagcag cgaattatac gcctgcaatt gctgaaagcc aaattagtag
     6241 aattccttta atcgttttaa caagtgaccg tccgcatgaa ttaagaagtg taggcgcacc
     6301 acaagcgatt aatcaagtaa atatgtttaa taattatgta agttatgagt tcgatatgcc
     6361 tattgcggat gatagtaaag agaccattga tgcaatttac tatcaaatgc aaattgctag
     6421 tcaatattta tatggaccac ataaagggcc aattcatttt aacttgccat ttagagatcc
     6481 gttaacacct gatttgaatg caacagaatt gttaacttct gagatgaaga ttttaccgca
     6541 ctatcaaaaa agtatagatg catcggcatt aagacacatt ttaaataaga aaaaaggttt
     6601 aattattgta ggggatatgc agcaccaaga agttgatcaa atactaacgt attcaacgat
     6661 atatgatttg cctattttag ctgatccttt aagtcattta agaaaatttg atcatccgaa
     6721 tgttatctgt acatatgatt tgctgtttag aagcggctta gacttaaatg tggatttcgt
     6781 aattcgtgtt gggaaaccag tgatttctaa aaagttaaat caatggttaa agaaaactga
     6841 tgcatttcaa atattagtgc aaaacaatga taagattgat gtctttccga tagcgccaga
     6901 tatttcatat gagatttctg cgaatgattt ctttaggtca ttaatggaag acacgacagt
     6961 taatcgcgta agttggttag aaaaatggca atgcttagag aaaaaagggc gtaaagaaat
     7021 taaatgttat ttggaacaag ctacagatga gagtgcattc gttggtgaat tgattaagaa
     7081 aacatctgaa aaagatgcat tatttattag taatagtatg cctatcagag atgtagataa
     7141 cttgttattg aataaaaata tagatgtcta tgcgaatcgt ggtgcgaatg gtattgatgg
     7201 tatcgtttca actgcactgg gtatggctgt gcataaacga ataacattat tgataggtga
     7261 tttatcattt tatcatgata tgaatggact attaatgtca aaattaaata atattcagat
     7321 gaatattgta ttattgaaca acgatggtgg cggtattttt tcatatttac cacaaaaaga
     7381 aagtgcaact gactattttg aacggttgtt tggcacaccg acgggattgg atttcgagta
     7441 tacagctaag ttatatcaat tcgattttaa acgttttaac agtgtttcag aatttaaaaa
     7501 tgccacattg ttatctgaaa cttcgacgat ttatgaattg ataacgaatc gcgaagataa
     7561 ctttaaacag catcaaattt tataccagaa attgagtgaa atgattcatg acacattata
     7621 aattttatga agcaaacgtt gagaccaatc aagttttagt atttctgcat ggatttctta
     7681 gcgacagccg tacttatcat aatcacatcg aaaaatttac tgataactat catgtcatca
     7741 ctatagactt accaggccat ggcgaagatc agtcttcaat ggatgaaacg tggaattttg
     7801 attatattac gacgttgtta gaccgaattt tagataaata taaagataaa tcaataacat
     7861 tgtttggata ttcaatgggt gggcgtgttg cattatatta tgcaattaat ggtcacatcc
     7921 ctatatctaa tttgatatta gaaagtacgt caccaggtat taaagaagaa gcaaatcaat
     7981 tggaacgccg tcttgttgat gatgcacgtg ctaaagtatt agacatagca ggtattgaat
     8041 tatttgttaa tgattgggaa aagttgccat tatttcaatc gcaactagaa ttaccagttg
     8101 aaatacaaca tcaaataaga caacaacgat tgtctcaatc gccacataaa atggccaaag
     8161 cattaagaga ttatggtaca ggtcaaatgc caaacttatg gccgcgcctg aaagaaatta
     8221 aagtaccaac attaatatta gctggagaat atgatgaaaa atttgtacag attgcgaaaa
     8281 aaatggcaaa tttaattcct aatagtaaat gtaaattaat ttctgctaca ggtcatacaa
     8341 ttcatgtgga agatagtgat gaatttgata caatgatatt aggattttta aaggaggagc
     8401 aaaatgacta acagacaatg ggaaacactt agagaatatg atgaaatcaa atatgaattt
     8461 tacgaaggga ttgctaaggt aacaataaat cgccctgaag tacgcaatgc gtttacacct
     8521 aaaacagttg ctgaaatgat tgacgcattt tcacgtgcac gtgatgatca aaacgtttca
     8581 gttatcgtat taactggtga aggtgattta gcattctgtt ctggtggtga ccagaagaaa
     8641 cgtggacatg gtggttatgt aggtgaagac caaatccctc gcttaaatgt attagattta
     8701 cagcgtttaa ttcgtattat tccaaaaccg gttatcgcga tggtaaaagg ttatgctgta
     8761 ggtggcggta atgtactaaa tgttgtttgt gacttaacga ttgctgctga taatgctatt
     8821 tttggacaaa ctggtcctaa agtaggttca tttgatgcgg gttatggttc aggatattta
     8881 gcacgtatcg ttggacataa gaaagcacgt gaaatttggt acttatgtcg tcaatacaat
     8941 gcacaagaag ctttagatat gggtctagta aatacagtgg tacctttaga gaaagttgaa
     9001 gatgaaactg tgcaatggtg taaagagatt atgaaacact caccaacagc gttacgattc
     9061 cttaaagcag ctatgaatgc tgacacagat ggtttagctg gtttacaaca aatggctggg
     9121 gatgcaacat tgctttatta cacaactgat gaagcgaaag aaggccgtga tgcgtttaaa
     9181 gaaaaacgtg atcctgactt cgatcaattc cctaaattcc cataagttat tttgaatatg
     9241 atattaagtc acttgcgtcg tttattagcg acgataggtg gcttttttat tttataagaa
     9301 tttaaaaggg cattttaatt aattatcata aagataagat agtttgagaa aaattaatag
     9361 agaaagaata aatgttaaag attattatta caatgaaaaa acgagctggt gagaaattga
     9421 attctcgcca acccgcttta tgaagtgaaa tcatttaaat tagttatact aagcgctcat
     9481 aaacgattgg tcgcgaagtg ccaatacctt gtttggatga cgtttgcatg aggatatgct
     9541 gtgtatcatc ataatcaaga atataaatga tttcttcatt atcaatagat gaaaatttaa
     9601 tgcgttgatg tgccgtatct ataaaaagaa tatgatattg attgtgactt gtatcatcgc
     9661 catgacgtat acaaattgat ttttgtaatt gatgattact ccaattacca atgaataaat
     9721 tgatattggt ttgttctaga tgtgtgagtt tggttgtgtc gtaaactaaa tttataaatt
     9781 gtagttgata catagtttta cctgccttta atcaatatct atattacttt ttagtaacct
     9841 atcattgaac cataccagtt ataatcacga ttgaatgata aatgtaatag gcttgaatct
     9901 gcatcttgga ttgataattc tgtatcccaa ggattccagt aaataagttt ttcttggtca
     9961 ttaattttag cattaccaac aactgctagc gcatgtccta aatgtgggtc attagggttt
    10021 tgagatacac tttgtgcaag gatcataatt cctacattat cttttgtaag ttgatcaact
    10081 tgttcatatg atggtacgcc ttcttgataa tgaatatctc tgccttgtga tttaccgtat
    10141 tcaatcattt gattagggaa tgttgcacaa ttaggaaggt cttgctcact tacttcaggg
    10201 tataatgtac gcataatatc atgtgcatta taagtgtctg tatttttagt tgcatttaat
    10261 aatgctgcca tactgaatcc tgcacaccat gagttatcga attgttgttc tctaattttg
    10321 aagtttttta atgtattttc atattgaact tgatcttctt gtatcgcttc gttttcttca
    10381 actttagtag gtgttactgt attttttaac tcttgtttca attttgctga aactgtttta
    10441 gcactttctt tttcttttac attaccaggt agaggcgtag cttttactaa tctaactttg
    10501 ccatcttctt caaaataaaa ccctttttca tcagtaagaa cagtgatatt tgaattttta
    10561 tctttaattt ggtctaaatc tttagcgatg aagtttgaaa ttttaacact gtaattcatg
    10621 tcttcttttg atttgtttaa atcatcttta tttttaggac taagtgttaa agtataaaca
    10681 attttaccgt cttttataac tggataatag taactattat cttcttcacc attaaattta
    10741 taaattttaa aagcttcgcc aagttcatat ttaccagttt ttgcattact ttgtttatct
    10801 aatgctttag cataaccagc aaattgttgt tgtgctagat cttttacttt ttgaggtact
    10861 ttgtcactct taacattaat ttctagctgt ttagagtgtg aatcggcttt ggctttattg
    10921 ttattagcaa atgcgcctag tgataaaata agcattgaaa ccattatgat gcttataata
    10981 ttgaatactc tagatttaca tgaactattc atataatacc ctccatttaa atcaaaatat
    11041 tttacgttgc taagactttg cttctatttt atctagacga acttaattgt taattaagct
    11101 gcatctggat tgtctgaatt attgttatcg ccattgtctg gattatcagg gttgttaggg
    11161 ttgtcagggt tatttggttc atctgggttg ttagggttat caggattgtt agggttatca
    11221 ggattatctg ggttattagg ttggtcatcg ttggcaaaat ggatatcttc aatattttgt
    11281 tttaagaagt tgcgtacatt ttcattaata aataccgcac cattaaattc atttggtaca
    11341 ccgccccaat gaattccgat cacttcattt ttttcattaa atacaggtga acctgaatta
    11401 ccaccagttg tacttaaatc atattgcata gcttcgcctt tgaggtaagt gatttttcct
    11461 ttactttccc acattgttgc tacaggttta tcaccaggat atcctgttac agtaatattt
    11521 tggttaactt gtgtttcagc attattactc attgttgctg gtttaactac ttcaccaata
    11581 tgtttgtttt gctcattagg ggagaattta actattgcta aatcaccttc gcctgaatat
    11641 ttagtgattt gttcagcagt gaaaccacca tttggataat tgtcttggtt aattgcagaa
    11701 gggaatgctt ttaaagcatg aggatcaccg tgcgtagcat ctacgacgtg tttatttgtt
    11761 aaaagagtat ctttacctac aactacaccg gaagcaataa atgtaccagt aggtgcttca
    11821 acttgaatat aagttacggg tgcataatga ccattcgttg tatctgtgat ttggtgacga
    11881 tcgttatttg gtaatataac atttgcgtgt tcacgttgtt ctaatggttt aaggttaccg
    11941 cctttttgaa tcttaggtgt ttgctgtttg cttgactgcg tttgttgtgg atgattgtcc
    12001 atagcctttg aagataacgc gtttgctgct ggagaactca caagtgtcgc tgttgtcaaa
    12061 gttgcaacga ataaagaact aacttttaaa aatttacctt tcatctaaaa acctccaaaa
    12121 aatttattta caagttaaat ataacactaa aaatttttaa gtcaataaga atatataaat
    12181 aaattaaaaa gtttttgtaa agattatagt ttacaaaaac attaagggaa ttaaaattga
    12241 attgtattat actttattca attgagttaa attgataaga tcgtcaattg tttgcattta
    12301 tctaaaagaa taaataagaa aggaattgag agcgaatgga aagtatttgt ttataataat
    12361 aaatagtaac ttttgaataa taattgtttg gccaaaggta gattaatatg aatggtgata
    12421 tattatttga gtgcttaagt ttcgaagaac aattataagt tagcatatta aaaccttttg
    12481 atagaatcat tttgagtgag gtgtttaaag gagaggaata taactcggta gtatttacag
    12541 tctctataga gtattattta aaacttatag aatataaaaa actgaacgta ctatgaattt
    12601 agttcacgac gtccagttca acatatcatt tttatttagt atttaaatat ttagcaagtc
    12661 tcttcatacc ttcttttaaa acatccattt cataagcata ggaaatcctt acaaatcctt
    12721 taccgaattc tgtaaaggat gagcctggaa cgattgctaa atgtgttgat tcaagtaaat
    12781 cgacacaaaa ttcgaaatcg tcatcggtga tatgtttaat acttgggaaa atataaaacg
    12841 cgccttcagg ttgagcggta atctcaaaac ctaatttagt taattcagat actaaataat
    12901 ttcttcgttc tacataagct tcgttcatat atttaggagc ttctaatcct tcgttaagtg
    12961 ctgttataca tgctatttga gctggaacat tggcacaaat acaattatag gcgtgcataa
    13021 atgttaattt atcaatcaaa tattgaggtc ctaatagaaa accaattctt attccggttg
    13081 ctgagtgtga tttacttaaa ccaccaatta atattaattg atcacgaatg tcttcaaatt
    13141 cagcgaagga tacatgttta ccactaaatg tattttcagc ataaatctca tcgctaataa
    13201 taaataacgg atattttttt aatacattta cgatatttaa aacttcattt ctttttaaaa
    13261 ctacgccagt tggattagtc ggataattta acaagacagc ttttgtcttt ggagaaatat
    13321 gactttctaa tgcatcaggt gtaattttaa attgtgttgc tgttgtatca atataaattg
    13381 gtttaccacc tagtacttcg atgagtggta tgtagcctgc ataaattggt cccggtatta
    13441 taatttcatc tccaggctct atgatagaac gtaacgttgt gtctattgct tcacttgctc
    13501 catttgtcac aataatttct tcaggatcat aggaaaaatg ataacgattt ttgaagtatt
    13561 gactaattgc ttcgcgagtt tctaataacc ctttattgtg agagtatgat gtcttgtcat
    13621 tgttaatagc atcaatatat gcctttttca caacatcagg catcgggaag tctggttggc
    13681 caatagttaa attaacacaa tcatccaaat tattcatacg atttgaaaat tggcgaatac
    13741 ttggtgctct taaatattta gaattagaat ttaaagaaag tttcatttgt gacacctcaa
    13801 atataaatca aatattgtct aaaaatttag aaattaataa tatcataaca tacttttaaa
    13861 gcaaaaaagg gtaaattact taagctttat ttgaaaaacg aaaaatgtct aaatacactg
    13921 tagtaactac ttttcaaaat gaataaagtg gttacagtta atgtacttag acagtataaa
    13981 attatgaatc tttaaactgt gatggtcttt gtaaattaat tattggattt gtccatttac
    14041 aaacaaagtt tgtagataat acatatacga tgattacaga tatacttatt aaataaagat
    14101 aagtcattaa tgatatagga ttatcgaatg ggtaccattc aaatcctcta acaatgccaa
    14161 taattaaacc atgtaataaa tatacgtata gcgtacgact accaatataa gtatataatt
    14221 ttttctttgt tgacattaaa tttagaaacg cagtcattgc gattaatata attccatata
    14281 atataagtcg tttaaaagga ctgaatatac tctgtccttc attttcaagt gaagtatatg
    14341 gtgaacttcc caataaccaa tctgcattga taggatgaat cacgtaaacg ataaaaaaca
    14401 aaataaaggt aatgatagat actggtatta gttttttatt tttaaaaata gccgtatgtt
    14461 ttttggtgaa aatgtaacct agataaaata ttgggaaaaa tacgattgtc cttgaaatgc
    14521 ttaagtagct atcgatgtta tctgaaaaac ctgctccaat agatataata attgaaactg
    14581 atagcacttt atatggatta aatcttctaa ctattactaa aatgacatga aagaaaaata
    14641 gcgtgatcaa aaaccataac gcaaatactg ggttaaaagg atcaagttgt aattcgtcac
    14701 ttttacctgt taagaaataa taaattgaaa agaatgcaaa aaatatcata taaggtacta
    14761 tcaaacgttt tgaaattttt tctaaatagt atggtttatc aatatttttc gcgaaataac
    14821 cagatataaa caaaaatgtt ggcatatgga aactataaat aactaggtat aatgctgata
    14881 aatatttatc gccactagtg tagggttgta acatatgtcc aaatacgact aataatatta
    14941 aaattgctct tgcgttgtca aaaaaataat ctctctcttt taatgaagtc atattttatc
    15001 tcctttatgt ccataagact cttactaatt tattatattt tgtattagtt atgcaacaaa
    15061 tttgtctatt gtgtcgaata gtattgtaaa aattataaaa aaagcatata atattataaa
    15121 taatatgtag tggagtagaa caacatgtat aaacaacttg aaaaacttat tacactgact
    15181 aacaatgact taaacttagt gaatagaaga tttggacaac gcacggatat caaatctgaa
    15241 cagctagaac ttctccgtat tttatttaat tacgatcgct tatcacagta tgatttgacg
    15301 atgaagatta gcagggaaca atctatagtt tcaaggtgga ttaagaaatt agttttgaaa
    15361 gggtacatca caagtcaaca atctagcgaa gatttaagat gtaaagaatt aattttgact
    15421 gatcaagcac gtacattaat ttcacaaata aataatgcac gttgcgaatt gattgaagca
    15481 agatgtcaat gtttatcgga agtcgaatta gacaatttaa atcaattact tgataagtta
    15541 aatcaacgac gcatatcgtt gtaacacgac aatagcaaac acataattta gtgtcgttgt
    15601 gcacacaaca aaaattgaat catgaattac aagcaaaagt agcggtgatt gttaaaattg
    15661 atggtaaaca atcaccgcta tttttgcttg tgtatgtata aaaaagggat caaaggtcat
    15721 cccccatgat tgatagtggg ggatgacttt tgatcctatg ttcatgttgc ttatttatat
    15781 tgtgggatgt cgaagtattt gccgacttcg ccaattttat catagtagcc tttgatgatt
    15841 ttagcattga tgttagccca atctacatct gtagcatatt ggtgtgttcc tggatgtgca
    15901 ggattccatc tcattttgta aagtgtattt tgaccagctt ttacatatga gttgccgatg
    15961 aatttagcac caccaacgat tgcttttgat actgtgtccc aaccagcttg tttagcatat
    16021 ttaatacctt cacgtaaagg atcgttatca tatgcagcaa taccaaatac gttatggtat
    16081 ttcgtgtttg agttagttac aactttgttg ttcactacat ctgcaccttt cgctaattga
    16141 gaagtaccgt tacctgtttc taatagggca tgtgagataa gataaacttc attaatgcca
    16201 tacatttgag cagctttgtt aaatgcagca ccttggtttt ctaatacacc tttacctttt
    16261 aagaattgat taattttatc aatagaaata ttttgtggtt ggtctaagcg taagaattga
    16321 tattttaatg ctggatcttg agctaaacgc ttcgtatcca ttgcatgctt aacatcatta
    16381 aagttagcat ctgtccactt acctggtaca cgttgtactt gtggtttata ttgtaaacca
    16441 gcttgtattt gagcaacttg gtttaatgtc atacctgttt gattatactt aattaattct
    16501 ttagctaaat cagttgattt aatccatgct aatttaccgt tagataattt accatagtac
    16561 caagtttgtc cattaatgac ttgttcttta acaactgcga atggttgttc attaaatgct
    16621 tttaatgagt atttagctgt atcagaattt ggtgttacat agtaataacc attaccattt
    16681 ttaattacat aagtgtagtt ataatctttg gcagctgatg tagttggttt cacagcggtt
    16741 ggtgcagtta aatcttttgc atttacccaa ccagtgcggt tattaatagt accgtataaa
    16801 taaacatctt tgcctacaga tacttgtttc gttgcattaa atgtaccttg agcaatgtta
    16861 ttgcctgtta aaatgacttg gtttttagta ccccaaggaa ccattgataa gccgttattt
    16921 gatttattaa cagtatattt ttgagtcgtt ttaacttctt tgcctaagtt ttgaacattt
    16981 aagtctttta cattgaacca acctaatggg atgttatggc ttgtattgtt taataataca
    17041 tacgtttcat taccatgagc acgctctttt gttacataga acgtacggtc tgcatatttc
    17101 gcaccgtttt tcgctgtttt ttcataaaca gaagcacgaa taccagtgtt gtttggttta
    17161 acttgagcaa tcttgctaac tgtttgagtc gtttgtggtt tagtaacagt ataagctttt
    17221 acagctgttt ttggttgtgc tactgctttt ttaggtgcag caggtacagc taaatatgct
    17281 ttacttaccc aaccagattt accatttaca gttccaaata aatagataga tttatcaatt
    17341 tgttgttgct tagtcgcttt aaaagtttgg ttacctgtac cagaaactgc accagcttct
    17401 tgtttataag tgccccaagg tactgaatat aatttagtgc ctggttttac tgtatatgtt
    17461 tgcattacat ttacaggtga ttttgcattg ttataaataa cgtcaccttg tttaacccaa
    17521 ccaattaaag ttggactatt gtaatcttta actaagtaga atttgtttcc acctaaactt
    17581 gcttcttttg ttacagcaaa tgttttttga acttctttcg ttggcttacc agttttgtca
    17641 taaactgtag tgaataagcc attgttttta gcattaattt gagcaacacc gtttaatgat
    17701 gaaactgtta atttattatt tgttgtaggt gttgatggct taggtgttgg tgtaggcgta
    17761 ggtttagcag tatcaactaa atatgcttta cttacccaac cagatttacc attcacagag
    17821 ccatataaat aaattgattt atcaatttgt tgttgctttg aagccttaaa tgtttggttt
    17881 ccagagccag acacactacc agcaacttgt ttagatgtac cccaaggtac tgtataaagt
    17941 ttcgtaccag gtttgattga atatgattga tttacattta caggtgattt agctgtgttg
    18001 taaaccacat cgccttcttt aacccaacca aatttattac cagaattgta atcttgaaca
    18061 agatagaatt tttgattacc taatgtagct gttttagata cagcaaatgt tttttgaact
    18121 tcattagttg ctttaccagt tttgtcgtat acagtagtat ataaaccact atttgttggt
    18181 ttgatttgtg cgacaccatt gtttgcagca actgttaatt taccagttga tggtttcgac
    18241 ggtgttgttg gttttgatgg tgtagtaggg gtagttgtag attgcgtacc ccatggcgcc
    18301 actttaccca tttttattaa atatttttca ttaattaagt catataattg atcataacta
    18361 taattatgac ttcttaaata tccatgtgga tcggcatggt cagtaccacc taaatattta
    18421 cttacagcgt agtgagtcca tactgtacca tttccatcat actcagcact gtctggtttt
    18481 aaaccataat attgtaattg tgtagctgca tagtcagcat agttattcat tgaacgtgca
    18541 aatgaagcat agtcgtgtgt gtgtacgatt tcaacattga tgaatctagg gttaccgact
    18601 gcaccgacac cccaagataa gtaatccgtt ggtgctgttt cgattatacg atccccatca
    18661 acaaatgcat gtacgaatgc gttttgatag ttatttttca tataactaat ttcaccattt
    18721 atcgtcgaac gatcattagc tgtatcatga actacgatac cttcaggacg acctacgccg
    18781 ttacggtatg cgtatttagg gaagtaagat gtataatctt cttcaatttt aggtgctttt
    18841 aagttatttt tacgaatgta atcgttaatt gaagagttta cttgtggttt atattttggc
    18901 aaactcgttt ttggtgttgc agcaactgat cttggttgtg ctgaagcgct aaaagtagtt
    18961 actttaggtg tcgcttcagt tttagcttta ggtgctgatg tagttgcagc tttaggtgct
    19021 gcggttttat attgcgtttc aagagctgca ggtttagcag ctgatttaat taattctgga
    19081 ttaatttgat tttctgaatt atcatcttca tcatcaacta aactataacc agcatttgta
    19141 acattagtgt tagttttagg tgctgtagtg cttgttgact ttgcaacagg ctgcgtatta
    19201 tttgtagtcg ctgattgatt agcacgagtg tcaccattta cttgtgcagt atcaactttt
    19261 tgacttactt gagcattgcc tgttttgtta tttgctgttt ttggttggac aatagcaggg
    19321 tcttgatata cttgagtgcc agaaatgttt tgcgttggat tttttacctc agcttttgct
    19381 tgttcagtag ttgctttaac tttattacta tctaaaacgt ttttattagt agtttgatct
    19441 tgtgtcgtct cagctgcttg aacttgatgt gcagtgactg ctgaacctac aagcgttaat
    19501 gcaaccattg atggtagttt gtaattgaat tttttcgcca ttctatttat tactcctaac
    19561 atttattaat tattactaac attatagtac ctgttttata tacctgtgcg tatttaacca
    19621 atttgtaata tactcttaat ttacattttc actgtaaaaa aatgaaaaaa attttgaatc
    19681 tcaatggtga cgtgacaaat caaatagaat tgatgtcaat ttaagaattg gtttattaca
    19741 aatgtttcta aaatagatac aacttttttt aattaagcga ggttaacttt tttgtcattt
    19801 caatatgctc gatgccttcc tcaagaaata tattacctct cattttaaag tttaaacttt
    19861 cataaaatgg gatagcatga cattgggcat tcatagtagc tacgtaaaaa ccttcatctt
    19921 tagctaatga ttctacagct tgcataagca ttctacccat tccttgtcca cgatgtgatt
    19981 tcatcacagc tactcgttct attttgacag ttgtttcatt aatagggcgt attcgagcag
    20041 tggcaactgg ctgtccatta tcatatccaa tgaggtgaat agattcagat tcatattcat
    20101 caatttcact ttcctcaggg acgccttgtt cttctacaaa cactttcttt cttatataga
    20161 agcaatcttc taacatcttt tgattgttta cttttgaaaa cataaaattc ctccacatcc
    20221 taacaaaatc atttacattt aatttactaa taataacata gaataacgat tgttattttc
    20281 ctcaaaaata attagacata aaaaagctat aaagatgatt gttgtgaatc atctttatag
    20341 ctaattaatt attaatgtta tagtgcttct tttctaataa ttgctgtata ttgccaaaag
    20401 attcgcattt gagcaatatt ccaattattc attcccattg taaatcctga aggcttaaat
    20461 atattcacat cagttacttc taaagctgct gcaactaaat attgtaatgg aatgctaagt
    20521 gatttcattg tttcggtaat gccattttca tcatataccc atgaaaatgg taactctgat
    20581 tcgaaaacat caacactatt gaaaatttca ggtagggcta aaatataaca tgcaccatct
    20641 acaataggat tgtcgatact atttttgtaa taaattagga gtgcttcata gttttctcga
    20701 tgctcatgat tgacgtaaaa aaattcactt ctaaaatgag ccatatcttg gctatgaatg
    20761 atttgttgtt ctaacatatt aaacatgcca tttatatttg caatcttttc gtatgttttt
    20821 ctactcatgt atgatgctcc tttcataaat agtaaatgtt aatcgtcata taatattaat
    20881 ttacaacacc attttggtta tttgaagctt gtggcgcttg ttgtgtgcca ccttgatttt
    20941 gatttgagtt ttgatctgta gcaggttgtt gttgattgct ggaatcactg ttattagttg
    21001 aatcactgtt ctcgttagat gtcttatctt tatctgtcgt atcattttgt tcttttctca
    21061 ataaactatt atctaaaggc gttaatggta ttaatgaacc ataatgatta atgacacgtt
    21121 gatctaagaa atcattttta tcattaatag gtgataattc taagtcttta cgaagtaagt
    21181 ttgcatattt ttgaatgctt tcaacacttg gatgataata gtaaatacca tttaacatat
    21241 catctttacc ttttaattgc gcagttttaa tttcaacatc atttgttaag tacatttttg
    21301 ctaaagcttt aatttcagag ttagttaaat tatgctttgc atttttacct acaatttgaa
    21361 tcacgttatc aagtttatca atagaatcaa cttcctgtgc tttttggaat aaaatcttaa
    21421 ttaattccat ttgacgttgt ccacgtttta agtctgaatc atgatgtcta gttctagcaa
    21481 ctgctaaagc ctcatcacca tttaattttt ggtacccttt tttaatttta atcttaccag
    21541 tatcatctgt gttaggttca tttaagtcgt atggcacatc atagtatatg ccaccaagct
    21601 catttacagc ctcgacaaat gctttcatat tgactctcac ataataatca acaggtacat
    21661 tcatggtagc ttctaccgaa tccattgcgg caattggacc accatatgca tgtgcatggg
    21721 taatcttatc gtaatagcca actttaggaa tgtagctgat agtatcacgt ggaatactaa
    21781 gcattctaat ttgatgtttt gattgattaa aagtagttaa aatcatagcg tctgatctag
    21841 agtgttcagc atcctgtcct ttttttcttc ttccatcgtt atcatcgata cctaagaaaa
    21901 gaatagagat aggttgttct tcgggattga ctttattatc tcttaagttg gattgacgat
    21961 tagcattttt gctgtcttga gaagattcga atgcatcttg ggacgtttta aaaagtaacg
    22021 tagcgaagac tattggaaca acaatgagaa ccaatgctag aaggatcaaa aagtatttta
    22081 aaaatttatt catgattgat gctcctatat taaatttttg ttaaatcata atcataattt
    22141 taaaatataa ttagttgaac gatatttata caacttcatt actaatacat tatagcctta
    22201 aattgtagta ctttatagta aaaaatacaa atgttatgtc aaagttcctt gtaatttgaa
    22261 tgttttgtta aaatcaaaaa gcatttttaa aaatataatg cgactttagt atgatgtctt
    22321 aaaaaattta aaggcgtaat ataaattcga gtgatttata caaagtgata aggtataatt
    22381 aattttgttg tatagataac atattagaaa aacttattac tatgtagcgt aaagcataaa
    22441 tgaatgataa gttaattttt gtaagttgta ttattaggca tctaggttca aatttttaac
    22501 gatttaaata tataaaatga atacattgtg aggcaattat caaatgaaat ttaataaagt
    22561 aaaactagtt atacatgcgt gtgtactatt atttatcatt atttctatag cgttaatttt
    22621 tcatcgatta cagacgaaga cacattctat agacccaata cataaggaaa caaaattatc
    22681 agacaatgaa aaatatttag tggatcgtaa taaggaaaag gttgcgccgt ctaaactaaa
    22741 agaggtatat aatagcaagg atcctaaata taagaaaatt gacaagtatt tacaaagttc
    22801 attatttaac ggttcagtag ctatatatga aaatggcaaa ttgaaaatga gtaaaggtta
    22861 tggatatcaa gattttgaaa aaggtattaa aaacacaccg aatacgatgt ttttaatagg
    22921 ttcagctcaa aaattttcaa cagggttact gttaaaacag ttagaagaag aacataaaat
    22981 aaatatcaat gatccagtaa gtaaatacct tccatggttt aaaacatcta agcctatccc
    23041 attgaaagat ttaatgttgc atcaaagtgg attatataaa tataaatcct caaaagatta
    23101 taaaaattta gatcaagcag ttaaagcgat tcaaaaacgt ggtattgatc ctaagaaata
    23161 caaaaagcat atgtataacg atgggaatta tttagtactt gcgaaagtaa ttgaagaagt
    23221 tacaggtaaa tcttatgctg agaattatta tacaaaaata ggagatcctt taaaacttca
    23281 gcacacagca ttttatgatg aacaaccttt taaaaaatat ctagcaaaag gttatgctta
    23341 taatagtaca ggactttcat tcttaagacc taatattttg gaccaatact atggtgcagg
    23401 taatttatat atgacaccaa cagatatggg taaattaatt actcaaatac aacaatataa
    23461 attattcagt cctaaaataa ccaatccatt attacatgag tttggtacga aaaagtatcc
    23521 agatgaatat cgatatggtt tctatgctaa gccaacattg aatagactta acgggggatt
    23581 ctttggacaa gtctttactg tttactataa tgataagtat gtagttgtac ttgcattaaa
    23641 tgtaaaagga aacaatga
//
LOCUS       DAKTIO010000018        23388 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.018, whole genome shotgun sequence.
ACCESSION   DAKTIO010000018 DAKTIO010000000
VERSION     DAKTIO010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 23388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23388
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.018"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..602)
                     /locus_tag="O0293_000582"
     CDS             complement(<1..602)
                     /locus_tag="O0293_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCY0279481.1"
                     /translation="MDNVTRYKITESSQSSSQAYYHLSFELSEQQSYASEHIVRAIRK
                     RQTILLYAVTGAGKTEMMFQGIQYARIQGDNIAIVSPRVDVVVEISKRIKDAFLNEDI
                     DILHQQSRQQFEGHFVVCTVHQLYRFKQHFDTIFIDEVDAFPLSMDKNLQQALKSSSK
                     VEHATIYMTATPPKQLLSEIPHENIIKLPARFHKKSLPVP"
     gene            complement(966..1832)
                     /gene="fakB1"
                     /locus_tag="O0293_000583"
     CDS             complement(966..1832)
                     /gene="fakB1"
                     /locus_tag="O0293_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB1"
                     /protein_id="HCY0279482.1"
                     /translation="MKIAVMTDSTSYLSQDLIDKYNIQIAPLSVTFDDGKNFTESNEI
                     AIEEFYNKMASSQTIPTTSQPAIGEWITKYEMLRDQGYTDIIVICLSSGISGSYQSSY
                     QAGEMVEGVNVHAFDSKLAAMIEGCYVLRAIEMVEEGYEPQQIIDDLTNMREHTGAYL
                     IVDDLKNLQKSGRITGAQAWVGTLLKMKPVLKFEDGKIIPEEKVRTKKRAIQTLEKKV
                     LDIVKDFEEVTLFVINGDHFEDGQALYKKLQDDCPSAYQVAYSEFGPVVAAHLGSGGL
                     GLGYVGRKIRLT"
     gene            1976..2617
                     /locus_tag="O0293_000584"
     CDS             1976..2617
                     /locus_tag="O0293_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HCY0279483.1"
                     /translation="MTQNIITVKKEHIIENVISKSRFIAHIKPVQNEDEAKAFIAAIK
                     KEHKDATHNCSAYTIGPEMNIQKANDDGEPTGTAGVPMLDILKKLDVHNACVVVTRYF
                     GGIKLGGGGLIRAYSGAVRDVIYDIGRVELREAVPCIVTLNYDQTGKFEYELASTHFM
                     LRNQTYTDKVSYYIDVVKSDYEAFINFLNQMTAGNFDLTEETPKQLPFDIPTE"
     gene            complement(2787..3842)
                     /locus_tag="O0293_000585"
     CDS             complement(2787..3842)
                     /locus_tag="O0293_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HCY0279484.1"
                     /translation="MVTLLLVAVTMIVSLTITPIVIAISKRLNLVDKPNFRKVHTKPI
                     SVMGGTVILFSFLIGIWIGHPIETEIKPLIIGAIIMYVLGLVDDIYDLKPYIKLAGQI
                     AAALVVAFYGVTIDFISLPMGTTIHFGFLSIPITVIWIVAITNAINLIDGLDGLASGV
                     SAIGLITIGFIAILQANIFITMICCVLLGSLIGFLFYNFHPAKIFLGDSGALMIGFII
                     GFLSLLGFKNITIIALFFPIVILAVPFIDTLFAMIRRVKKGQHIMQADKSHLHHKLLA
                     LGYTHRQTVLLIYSISILFSLSSIILYVSPPLGVVLMFVLIIFSIELIVEFTGLIDNN
                     YRPILNLISRKSSHKEE"
     gene            4160..5230
                     /locus_tag="O0293_000586"
     CDS             4160..5230
                     /locus_tag="O0293_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCY0279485.1"
                     /translation="MFEAFIYNISVIVAGIYLFHRLQYSENKRMVFSKAYVTVLMTIV
                     SLLLSVYPIPYREDYLIHLTFVPLLFLGRFTNMVYTLSATVIVAIVEIVVFNNSIMYG
                     VTLIVIAAVTSAIGPFLKQNDVLSLLILNVVTIIILFGVALVSPIYTLSEVIILIPIS
                     LIITLASAITFVDIWHFFSLVNRYENEDKYDYLTGLGNVKEFDRHLNEISRKAEKEHQ
                     SIALLLIDIDGFKDVNDTYSHKSGDAVLKQMSQLLKNYVPNQFKIFRNGGEEFSVVIH
                     NYSLDQSVKLAENIRSGVEKSSFHLPNKEVIKLSVSIGVGYLTDDDPKSQRKVFKDAD
                     DMVHVAKNQGRNKVMFNPIINL"
     gene            5426..6187
                     /locus_tag="O0293_000587"
     CDS             5426..6187
                     /locus_tag="O0293_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCY0279486.1"
                     /translation="MPDSITIIDENKVIDVVLIAGRILLESGAETYRVEDTMNRIAHS
                     YGLHNTYSFVSSTAIIFSLNDRTSTRLIRVQERTTDLEKIALTNSLSRKISNKELTID
                     EAKSEFIHLQHASLQYSFLTNFFAAAIACGFFLFMFGGVASDCWIAVIAGGSAFLTFS
                     FVQRYIQIKFFSEFVAAAVVISIAATFTKLGIATNQDIITIASVMPLVPGILITNAIR
                     DLLAGELLAGMSRGVEAALTAFAIGAGVAIVLLII"
     gene            6205..6699
                     /locus_tag="O0293_000588"
     CDS             6205..6699
                     /locus_tag="O0293_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCY0279487.1"
                     /translation="MLFYLFHFTISFISTVLFSIIFNAPKRLLVACGFVGAIAWTIYQ
                     LTVDLEFGKVGASFLGSLILGLMSHTMSRRYKRPVIIFIVPGIIPLVPGGAAYQATRF
                     LVSNDYTSAVNTFLEVTLISGAIAFGILVSEILYYLYTRIKQLYGKIKGKTYKKSYNM
                     NNRV"
     gene            6713..7939
                     /gene="pepT"
                     /locus_tag="O0293_000589"
     CDS             6713..7939
                     /gene="pepT"
                     /locus_tag="O0293_000589"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCY0279488.1"
                     /translation="MKNQLIDRLTRYTTIDTQSDPKSTTTPSTEKQWDLLHLLEKELQ
                     QLGLPTDLDENGYLFATLESNIDVDVPTVGFLAHVDTSPDFNASNVKPQIIENYDGKP
                     YKLGNTKRVLDPKVFPELNSLVGHTLMVTDGTSLLGADDKAGIVEIMEAICYLQEHPE
                     IKHGTIRIGFTPDEEIGRGPHKFDVDRFNADFAYTMDGSQYGELQYESFNAAEAVITC
                     HGVNVHPGSAKNAMVNAIRLGEQFDSLLPDSEVPERTEGYEGFYHLMNFEGTVEKATL
                     QYIIRDHDKKQFELRKKRILEIRDDINAHFENYPVKVDISDQYFNMAEKILPLPHIID
                     IPKRVFAKLDIPANTEPIRGGTDGSQLSFMGLPTPNIFTGCGNFHGPYEYASIDVMEK
                     AVQVIIGIVEDIAENH"
     gene            complement(8128..9252)
                     /locus_tag="O0293_000590"
     CDS             complement(8128..9252)
                     /locus_tag="O0293_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCY0279489.1"
                     /translation="MKVLVAMDEFHGIISSYQANRYVEEAVASQIETADVVQVPLFNG
                     RHELLDSVFLWQSGQKYRIPVHDADMNEVEGVYGQTDTGMTVIEGNLFLKGKKPIVER
                     TSYGLGEMIKHALDNDAKHVVISLGGIDSFDAGAGMLQALGAQFYDDEGRVVDMRQGA
                     GVIKYIRRMDMSNLHPKMETARIQVMSDFSSRLYGKQSEIMQTYDAYQLNHNQAAEID
                     NLIWYFSELFKSELKIAIGPVERGGAGGGIAAVLNGLYQAEILTSHALVDQLTHLENL
                     VEQADLIIFGEGLNENDQLLETTTLRIAELCHKHQKVAIAICATAEKFDLFESQGVTA
                     MFNTFIDMPETYTDFKMGLQIRHYTVQSLKLLKTHFNVEV"
     gene            complement(9677..9997)
                     /gene="ytxJ"
                     /locus_tag="O0293_000591"
     CDS             complement(9677..9997)
                     /gene="ytxJ"
                     /locus_tag="O0293_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HCY0279490.1"
                     /translation="MAIKLSSIDQFEQVIEENKYVFVLKHSETCPISANAYDQFNKFL
                     YERDMDGYYLIVQQERDLSDYIAKKTNVKHESPQAFYFVNGEMVWNRDHGDINVSSLA
                     QAEE"
     gene            complement(10151..11029)
                     /locus_tag="O0293_000592"
     CDS             complement(10151..11029)
                     /locus_tag="O0293_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EMYY motif lipoprotein"
                     /protein_id="HCY0279491.1"
                     /translation="MKKIVIIAVLAILFVVISACGNKEKEAQHQFTKQFKDVEQKQKE
                     LQHVMDNIHLKEIDHLSKTDTTDKNSKEFKALQEDVKNHLIPKFEAYYKSAKNLPDDT
                     MKVKKLKKEYMTLANEKKDAIYQLKKFIGLCNQSIKYNEDILDYTKQFEKNRYKVESE
                     IKLADNKSEATNLTTKLEHNNKALRDTAKKNLDDSKENEVKGAIKNHIMPMIEKQITD
                     INQTNISDKHVNNARKNAIEMYYSLQNYYNTRIETIKVSEKLSKVDVDKLPKKGIDIT
                     HGDKAFEKKLEKLEEK"
     gene            11149..11667
                     /locus_tag="O0293_000593"
     CDS             11149..11667
                     /locus_tag="O0293_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HCY0279492.1"
                     /translation="MYSNVQPFINSDLTNCFQSQYTEIESLLFNLLDSPVKYTKHIGG
                     THHFKYSTEPILDILVGVENLHDITSLDEKRLNYVGFYRLHHKYTKKVMMAKFNNFHE
                     LKQQVRLHIIQMNTQTFESYLALDYLLSTNDDIALQFNTKKHQILQHSLTIRQYEMQK
                     QQYFEKLCKKYL"
     gene            11794..12717
                     /locus_tag="O0293_000594"
     CDS             11794..12717
                     /locus_tag="O0293_000594"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCY0279493.1"
                     /translation="MINKDIYQALQQLIPNEKIKVDEPLKRYTYTKTGGNADFYITPT
                     KNEEVQAVVKYAYQNEIPVTYLGNGSNIIIREGGIRGIVISLLSLDHIEVSDDAIIAG
                     SGAAIIDVSRVARDYALTGLEFACGIPGSIGGAVYMNAGAYGGEVKDCIDYALCVNEQ
                     GSLIKLTTKELELDYRNSIIQKEHLVVLEAAFTLAPGKMTEIQAKMDDLTERRESKQP
                     LEYPSCGSVFQRPPGHFAGKLIQDSNLQGHRIGGVEVSTKHAGFMVNVDNGTATDYEN
                     LIHYVQKTVKEKFGIELNREVRIIGEHPKES"
     gene            12735..13049
                     /locus_tag="O0293_000595"
     CDS             12735..13049
                     /locus_tag="O0293_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279494.1"
                     /translation="MPKVYGSLIDTETRCRHYFTEEDIIAIKFKCCNKYYPCYKCHNE
                     FEKHAIKRWSEPSFNEKAILCGVCKHELTINEYMMVERCPNCQSRFNNRCKYHYHIYF
                     EI"
     gene            complement(13366..14394)
                     /locus_tag="O0293_000596"
     CDS             complement(13366..14394)
                     /locus_tag="O0293_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HCY0279495.1"
                     /translation="MKKTVLYLVLAVMFLLAACGNNSDKEQSKSETKGSKDTVKIENN
                     YKMRGEKKDGSDAKKVKETVEVPKNPKNAVVLDYGALDVMKEMGLSDKVKALPKGEGG
                     KSLPNFLESFKDDKYTNVGNLKEVNFDKIAATKPEVIFISGRTANQKNLDEFKKAAPK
                     AKIVYVGADEKNLIGSMKQNTENIGKIYDKEDKAKELNKDLDNKIASMKDKTKNFNKT
                     VMYLLVNEGELSTFGPKGRFGGLVYDTLGFNAVDKKVSNSNHGQNVSNEYVNKENPDV
                     ILAMDRGQAISGKSTAKQALNNPVLKNVKAIKEDKVYNLDPKLWYFAAGSTTTTIKQI
                     EELDKVVK"
     gene            complement(14513..15274)
                     /locus_tag="O0293_000597"
     CDS             complement(14513..15274)
                     /locus_tag="O0293_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0279496.1"
                     /translation="MIQVENLTKTINNQMILEDISIDIEKGKLTSLIGPNGAGKSTLL
                     SAICRLIRFENGEVKIDGQLMSDYKNNDLSKKISILKQTNHTEMNITVEQLVNFGRFP
                     YSKGRLTKEDHDIVNDALDLLQLQDIRNRNIKSLSGGQRQRAYIAMTIAQDTEYILLD
                     EPLNNLDMKHAVQIMQTLKMLAHKMNKAIVIVLHDINFASCYSDQIVALKNGQLVKSD
                     LKDNVIQSSVLSDLYDMNIQIEHIRNQRICLYFKD"
     gene            complement(15271..16227)
                     /locus_tag="O0293_000598"
     CDS             complement(15271..16227)
                     /locus_tag="O0293_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCY0279497.1"
                     /translation="MRNNNKKIMLLIAVTLLISMLYLFVGIDFEIFEYQFSSRLRKFI
                     LIILVGAAIATSVVIFQAITNNRLLTPSIMGLDAVYLFIKVLPVFLFGIQSVWVTNVY
                     LNFILTLITMVLFALILFQGIFKIGHFSIYFILLIGVLLGTFFRSITGFIQLIMDPES
                     FLAIQSSMFANFNASNSNLVTFSAVLLVILLVITILLLPYLDVLLLGRAEAINLGISY
                     EKLTRILLVIVSVLVSVSTALVGPITFLGLLTVNLAHELMKTYEHKYILIATICLSWI
                     SLFSAQWVVENVFEATTEMSILIDLIGGSYFIYLLVRRRNAQ"
     gene            complement(16214..17185)
                     /locus_tag="O0293_000599"
     CDS             complement(16214..17185)
                     /locus_tag="O0293_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0279498.1"
                     /translation="MKYLLKGNILLLLLILLTIISLFIGVSELSIKDLLHLTESQRNI
                     LFSSRIPRTMSILIAGSSLALAGLIMQQMMQNKFVSPTTAGTMEWAKLGILIALLFFP
                     TGHILLKLVFAVICSICGTFLFVKIIDFIKVKDVIFVPLLGIMMGGIVASFTTFISLR
                     TNAVQSIGNWLNGNFAIITSGRYEILYLSIPLLALTYLFANHFTIVGMGKDFTNNLGL
                     SYEKLINIALFITATITALVVVTVGTLPFLGLVIPNIISIYRGDHLKNAIPHTMMLGA
                     IFVLFSDIVGRIVVYPYEINIGLTIGVFGTIIFLILLMKGRKNYAQQ"
     gene            complement(17224..17379)
                     /locus_tag="O0293_000600"
     CDS             complement(17224..17379)
                     /locus_tag="O0293_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279499.1"
                     /translation="MFYFTLSKQNTHSEKLNFSLIANINKIDTKLFERLLKLWSKNAT
                     INEKYYQ"
     gene            complement(17893..18864)
                     /gene="nrdF"
                     /locus_tag="O0293_000601"
     CDS             complement(17893..18864)
                     /gene="nrdF"
                     /locus_tag="O0293_000601"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCY0279500.1"
                     /translation="MIAVNWNTQEDMTNMFWRQNISQMWVETEFKVSKDIASWKTLSE
                     AEQDTFKKALAGLTGLDTHQADDGMPLVMLHTTDLRKKAVYSFMAMMEQIHAKSYSHI
                     FTTLLPSSETNYLLDEWVLEEPHLKYKSDKIVANYHKLWGKEASIYDQYMARVTSVFL
                     ETFLFFSGFYYPLYLAGQGKMTTSGEIIRKILLDESIHGVFTGLDAQHLRNELSESEK
                     QKADQEMYKLLNDLYLNEESYTKMLYDDLGITEDVLNYVKYNGNKALSNLGFEPYFEE
                     REFNPIIENALDTTTKNHDFFSVKGDGYVLALNVEALQDDDFVFDNK"
     gene            complement(18982..21087)
                     /gene="nrdE"
                     /locus_tag="O0293_000602"
     CDS             complement(18982..21087)
                     /gene="nrdE"
                     /locus_tag="O0293_000602"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCY0279501.1"
                     /translation="MKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVK
                     DKTIFFDTEIERLRYLVDNDFYFNVFDIYSEADLIEITDYAKSIPFNFASYMSASKFF
                     KDYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFISAMVEQRYQPATPTFLNA
                     GRARRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIK
                     GVAKGVLPIAKSLEGGFSYADQLGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRL
                     STISTGLIVPSKFFDLAKEGKDFYMFAPHTVKEEYGVTLDDIDLEKYYDDMVANPNVE
                     KKKKNAREMLNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLCTEIFQLQET
                     SIINDYGIEDEIKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAP
                     GVRKANSELHSVGLGVMNLHGYLAKNKIGYESEEAKDFANIFFMMMNFYSIERSMEIA
                     KERGIKYQDFEKSDYANGKYFEFYTTQEFEPQFEKVRELFDGMAIPTSEDWKKLQQDV
                     EQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQTM
                     WYYKSAFNTDQMKLIDLIATIQTHIDQGISTILYVNSEISTRELARLYVYAHYKGLKS
                     LYYTRNKLLSVEECTSCSI"
     gene            complement(21050..21448)
                     /gene="nrdI"
                     /locus_tag="O0293_000603"
     CDS             complement(21050..21448)
                     /gene="nrdI"
                     /locus_tag="O0293_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCY0279502.1"
                     /translation="MKIIYFSFTGNVRRFIKRTELENTLEITAENCMEPVHEPFIIVT
                     GTIGFGEVPEPVQSFLEVNHQYIRGVAASGNRNWGLNFAKAGRTISEEYNVPLLMKFE
                     LHGKNKDVIEFKNKVGNFNENHGREKVQSY"
     gene            22249..23115
                     /locus_tag="O0293_000604"
     CDS             22249..23115
                     /locus_tag="O0293_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCY0279503.1"
                     /translation="MNPKVKGIIAILISAIGFSFMSVFFRLAGDLPVFQKSLARNLVA
                     MFIPLFFIYKYHQPMFGRLSSQPLLITRSTLGLIGVLLNIYAIDHMVLSDADSLMKLN
                     PFWTILLSIVFLHEKVRKYQITAMIIAILGMLLIVKPEFSSSMIPSLAGLFSGIFAAS
                     AYTCVRALSTREAPYTIVFYFSLFSVIVLIPFTAYTYEPMSQMQILYLLGAGLAAAVG
                     QIGVTLAYSFAAAKDISIFTYASIIFTAILGFILFGESPDFYATLGYVVIIGASYYMF
                     EKARRDAKIIKK"
     gene            23135..>23388
                     /gene="queF"
                     /locus_tag="O0293_000605"
     CDS             23135..>23388
                     /gene="queF"
                     /locus_tag="O0293_000605"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HCY0279504.1"
                     /translation="MAHGRQQDELQDITLLGNQDNTYNFDYRPDVLESFDNKHQGRDY
                     FVKFNCPEFTSLCPITGQPDFATIYISYIPNVKMVESKSL"
BASE COUNT         7725 a         4032 c         3391 g         8240 t
ORIGIN      
        1 ttaggaactg gaagtgattt tttatgaaag cgagctggca atttaattat attttcgtgg
       61 ggaatctctg acagaagttg tttcggtggt gttgctgtca tataaattgt tgcatgttca
      121 actttagaag atgacttcaa tgcttgttgt aaatttttat ccattgataa aggaaaggca
      181 tcgacttcat caataaaaat agtatcaaag tgctgtttga atcggtaaag ttgatgcact
      241 gtgcatacaa caaaatgccc ttcaaattgt tgtcttgatt gctggtgtag tatgtctata
      301 tcttcattaa gaaatgcgtc tttaatacgt ttactaattt ctacaacaac atctacacgt
      361 ggtgacacaa tagctatatt atctccctgt attcttgcat attgaatgcc ttgaaacatc
      421 atttctgtct tacctgcacc tgttacggca tataacaaaa tcgtttgtct ctttctaatg
      481 gctcgaacaa tatgttctga ggcataagac tgctgttccg acaattcaaa tgagagatga
      541 taatatgctt gtgatgaact ttgcgaactc tctgttattt tatatcttgt tacattatcc
      601 attcgaccca gtgatataca atttctgcaa tatacaattt tcttggaatg tactgaggaa
      661 tcatactgat aaaaatgctt tggatttgta ctttcacatt gaacacaata atagtttgaa
      721 ttttgtttga tgacacctgt tacttctttt ttaatttttt cattagtaag tgaagtatga
      781 ggatgaatta atttcccgta atgtttaatg cttaaaacct catttcaacg taaaaaagag
      841 cctaatatta taatgatttt cttatatctt tagcattttg gctttcaaat gatgaacatt
      901 taaagccatg tatacgagag ataatttcca attacaatat agactctttt attaaaattt
      961 tataattatg ttaatcttat ttttctgcca acatagccta aacctaatcc accagaacct
     1021 aaatgtgctg caacaactgg accaaactca gagtatgcta cttgataagc tgaaggacaa
     1081 tcatcttgta actttttgta taacgcttga ccatcttcga aatgatctcc atttatgaca
     1141 aataaagtta cttcttcaaa gtcttttaca atatctaata ctttcttttc taatgtttga
     1201 atggcacgct ttttagtacg aactttttct tctggtataa tcttgccatc ttcaaactta
     1261 agaactggct tcattttcaa taatgttcca acccatgctt gagcaccagt aattcgacca
     1321 cttttttgta aattctttaa gtcatcaaca attaaatatg cacctgtgtg ttcacgcata
     1381 ttagttaaat catcaataat ttgctgtggc tcgtatcctt cttcaaccat ttcaatagca
     1441 cgtaatacat aacatccttc aatcattgct gcaagcttac tatcaaatgc atgtacatta
     1501 acaccttcaa ccatttcccc tgcttgataa ctagattgat aacttccact aatcccactt
     1561 gataagcaaa tgacaatgat atctgtgtaa ccttgatctc ttaacatttc atatttagta
     1621 atccattcgc caattgctgg ttggcttgtt gttggaatcg tttgagacga tgccatttta
     1681 ttataaaatt cttcaattgc tatttcatta ctttctgtaa agttcttccc atcatcaaaa
     1741 gtcacactta atggcgctat ttgaatatta tatttatcga ttaagtcctg cgacagataa
     1801 cttgtagaat cggtcatcac agcaattttc atagtcgtcc tcctaaaata tactcatagc
     1861 aatgatacaa tatttcagtt aaaaagaaaa tggcaatcaa acgatttata gtcttgtcat
     1921 tttcagtgta taatgaagat taaataatct taagcttatg aattaaggag ttattatgac
     1981 acaaaatatc attactgtta aaaaagaaca cattattgaa aatgtgatta gtaaatcaag
     2041 attcattgct catatcaaac ctgttcaaaa tgaagatgaa gctaaagcat ttattgctgc
     2101 aataaaaaaa gaacataaag atgccactca taattgttct gcatatacta ttggacctga
     2161 aatgaatatt caaaaagcga atgatgatgg tgaaccgaca ggaactgccg gtgtcccaat
     2221 gttagacata ttaaaaaagc tcgatgttca taatgcgtgt gtcgtagtaa caagatattt
     2281 tggtggtatt aaattaggtg gtggcggttt aattcgcgcc tatagcggtg ctgttagaga
     2341 cgtcatttat gatatcgggc gtgtcgaact acgagaagca gtaccatgta tagtaacact
     2401 gaattatgat caaactggta aatttgaata tgaattagct tcaactcatt ttatgttaag
     2461 aaaccaaact tacactgata aggtaagcta ctatatcgat gtcgttaaaa gtgactatga
     2521 agcgtttatt aactttttaa atcaaatgac tgcaggaaat ttcgatttga ctgaagaaac
     2581 tccaaagcag ttacctttcg atataccaac tgaataaaaa taaaaagcac tatcataaga
     2641 cggtaaatat caattgcgta ttattaaacg acaattgaag tacacgttta tggcagtgct
     2701 ttttatattt gaaatttaat acaaaaaaat tgtaaagagt aaagccatat aatacaaatt
     2761 atcccataca gctatgcttt cattccctat tcctctttat gagatgactt acgactaatt
     2821 aaatttaata ttggtcggta gttgttatct attaatcctg taaattcaac aattaattca
     2881 atactaaata tgattaatac aaacattaat acaacaccta atggtggcga tacatacaaa
     2941 ataatgctcg aaagactaaa taaaatagag attgaataga ttaataatac tgtttgtcta
     3001 tgtgtgtagc ctaaagctaa tagtttatga tgcaaatgcg atttatcagc ttgcattata
     3061 tgctgccctt ttttcacacg tcgaatcatt gcgaacaaag tatcaatgaa tggaactgct
     3121 aagataacaa ttgggaagaa caatgcaata attgtaatat ttttgaatcc gagtaaagaa
     3181 aggaatccga tgataaatcc aatcattaaa gccccactat cacctaaaaa tattttggca
     3241 ggatggaaat tgtaaaataa aaacccaatt aaagagccta ataaaacaca acaaatcatc
     3301 gttatgaaaa tattagcttg taaaattgca atgaacccta ttgtaatgag tccgattgca
     3361 gaaacacccg acgccaaacc atcgagtcca tcaattaagt taattgcatt tgtaatagca
     3421 acaatccaaa tcacagtaat tggaatacta agaaatccaa aatgaatcgt tgtacccatt
     3481 ggcaacgaaa taaaatcaat agtcacacca taaaaagcaa ctactaaggc agcggcaatt
     3541 tgaccagcca attttatata cggtttcaaa tcgtagatat catctacaag cccaagtacg
     3601 tacataataa tcgcaccaat aataagtggt ttgatttctg tttcaatagg atgaccaatc
     3661 caaataccta ttaaaaatga aaagagaatc actgtaccac ccataactga aataggttta
     3721 gtgtgtactt ttctaaaatt tggtttatca actaaattta atcttttcga tattgcaata
     3781 acaattggtg ttatcgtcaa actgacaatc attgttactg caactagtaa taatgtaacc
     3841 atttattcac cttcatcgat attaattgtt tttaacgtta tatttattta atattgtctt
     3901 tatagaatga tatatctatt ttctacgtat atcttatagc tttattataa attaattcgt
     3961 tcaatcaatg atatcatatt tacaactata cgattcattt cttatatatc gcctattatt
     4021 cattttttca tcatatcatt taaggcaaca ttttaacatc aaagtctata tcatacatta
     4081 attttatatg taaactaatg acaaattgta agttttcact tacaatagct agtggtcaat
     4141 taaaggagtc gattcagaca tgttcgaagc atttatatac aatatatctg ttatagtcgc
     4201 tggaatctat ttattccatc gcttacagta ttcagaaaac aaacgtatgg ttttttctaa
     4261 ggcttatgtc actgttttaa tgacaatcgt atcattatta ttatctgtct accctatccc
     4321 ttatcgtgaa gattatttaa ttcacttaac attcgttcct ttattgtttt taggtagatt
     4381 tacaaatatg gtctatacat tatctgccac agtaattgta gcaattgttg aaattgtagt
     4441 atttaacaac tcgattatgt acggtgttac gttgattgtt attgctgcag taacaagtgc
     4501 tattggacca tttttaaaac aaaatgatgt gctatcatta ttaatactta atgttgttac
     4561 aataatcatt ttatttggtg tcgcgttagt gagtcctatt tacacactat cggaagttat
     4621 cattttaata ccaatttcat taattatcac tttagcttca gcgataacat ttgtcgatat
     4681 ttggcatttc ttctcactcg taaatcgtta tgaaaacgaa gataaatatg attacttaac
     4741 aggcttaggt aatgtaaaag aatttgatag acatttaaat gaaatttcac gaaaagctga
     4801 aaaagaacat caaagtatcg cattattatt aatcgatatc gatggattta aagatgtcaa
     4861 tgatacctac tcacacaaat caggtgatgc tgtattaaaa caaatgtctc aattacttaa
     4921 aaactatgtg ccgaatcaat ttaaaatttt tagaaatggt ggcgaagagt tctcagttgt
     4981 cattcacaat tattcacttg atcaaagtgt taaattagct gaaaatattc gttcgggtgt
     5041 tgaaaaatca tcattccatt taccaaacaa agaagttatt aaattgtcag tgtctatcgg
     5101 tgttggttat ttaacagatg acgatccaaa atcacagcgt aaagtgttta aagatgcaga
     5161 cgatatggtg catgtagcca aaaatcaagg gcgaaacaaa gtaatgttta accctattat
     5221 caatttataa caacgacaaa actgttaatc ctttgctatt attaaatcat agtataatag
     5281 caaagattaa cagtttttct tatttaaatt tgaaccatac aagttcatca tactaacaca
     5341 aagtctcact attgaaagtt gtaacaacaa gttgtaaact tcaattgttc aaataaataa
     5401 cacatatcat tggaggtgaa ttgatatgcc agattcaatc acaattatag atgaaaacaa
     5461 agtgattgat gttgtattaa ttgcaggtag aattttactt gaatcaggtg ctgaaacata
     5521 tcgagttgaa gatacaatga accgtatcgc acatagttat ggtcttcata atacatatag
     5581 ttttgtcagt tcaactgcaa ttattttttc attaaacgat cgaacaagta caagattaat
     5641 tcgtgtacaa gagcgtacaa cagatttaga gaaaatcgct ttaacgaata gtctttcacg
     5701 taaaatatca aataaagaac tcacaattga tgaagctaaa tctgaattta ttcatttaca
     5761 gcatgcatca ttacaatatt cttttttaac gaatttcttt gcagctgcca ttgcatgtgg
     5821 cttcttccta tttatgtttg gtggtgtcgc atcagattgt tggattgcag tcattgctgg
     5881 cggatccgca tttttaacat tcagctttgt gcaacgctat atacaaatta aatttttctc
     5941 agagtttgtt gcagccgctg tggtcatatc cattgcagcc acatttacta aattaggcat
     6001 tgcaaccaat caagatatca ttactatagc cagtgtcatg ccacttgtcc ctggtatatt
     6061 aattaccaat gccattcgag atttattagc gggtgaatta cttgcaggta tgtctcgcgg
     6121 tgttgaagct gcattaacag cattcgcaat cggtgctggt gtcgcaatcg ttttattaat
     6181 catttaagga aggactgtta aagcatgcta ttttatttat ttcattttac aatcagcttt
     6241 atatcaacag tacttttctc tatcattttc aatgcaccca aacgcctctt agtagcatgt
     6301 ggatttgtgg gtgccattgc atggacgatt taccaattaa cggtagattt agagtttgga
     6361 aaagttggcg cttcattttt gggaagctta attttaggct taatgagtca tactatgagt
     6421 cgcagatata aacgaccggt aattatattc atagtgccag gcattatacc attagtacct
     6481 ggtggtgcag cttatcaagc gactcgtttt ttagtatcaa atgattatac aagtgctgta
     6541 aatacatttt tagaagttac actgatttca ggtgcgattg ctttcggtat attagtttct
     6601 gaaattctat attacctata cacacgtatc aaacaactgt atggtaaaat caaaggtaag
     6661 acatataaaa aatcttacaa catgaataat agagtttaag gggtcgaata taatgaagaa
     6721 ccaattgata gatagattaa caagatatac gacaattgat acgcaatctg atccaaaatc
     6781 tacaacaaca ccttcaactg aaaagcaatg ggatttgtta catttattag aaaaagaatt
     6841 acaacaatta ggattgccaa ctgatttaga tgaaaatggc tacttatttg ctacattaga
     6901 aagtaacatc gatgtcgacg tcccaacagt tggatttttg gcgcatgttg atacatcacc
     6961 tgatttcaat gcttcaaatg ttaaaccgca aattattgaa aactatgatg gtaagccata
     7021 caaactaggc aatactaaac gtgtcttaga tcctaaagta tttccagaac ttaacagttt
     7081 agttggtcat acattaatgg taactgatgg tacatcgtta ctaggtgcag acgataaagc
     7141 gggtattgtg gaaattatgg aagctatttg ttatttacaa gaacatccag aaattaaaca
     7201 tggtaccatt cgcattggat ttacaccaga cgaagaaatc ggtcgtggtc cacataaatt
     7261 tgatgttgac cgcttcaatg ctgattttgc ttatactatg gatggtagtc aatatggaga
     7321 attacaatat gaaagcttta acgctgctga agcggttatt acatgccacg gtgtaaatgt
     7381 tcatcctggt tcagctaaaa atgcaatggt aaacgcaata cgtttaggtg aacaattcga
     7441 tagtttgcta cctgatagtg aagttccgga gcgaacagaa ggatacgaag gcttttatca
     7501 cttaatgaac tttgaaggaa ctgttgaaaa agcaactttg caatacatta ttcgtgatca
     7561 tgataaaaaa caattcgaat tgcgtaagaa acgtatttta gaaatacgtg acgatatcaa
     7621 tgcccatttt gaaaattatc cagttaaagt tgatatatcg gatcaatatt tcaatatggc
     7681 agaaaaaata ttaccattgc ctcatattat tgatatacct aaacgtgtct ttgccaaatt
     7741 agatattcca gcaaacactg aacctattcg cggtggtaca gatggttcac aattgtcatt
     7801 tatggggtta ccaactccaa acatctttac aggttgcggt aacttccacg gcccatatga
     7861 atatgcatcc attgatgtta tggaaaaagc agtacaagta atcattggaa ttgtagaaga
     7921 tatcgctgaa aatcactaaa aaccaacgat tatatactag ttcaactcag gcttaattaa
     7981 tatataaaaa tcacgctaac attcagtgca atgatagcgt gatttttcat attttttacg
     8041 tatactttcg atcttctaat tcgttttgct aaccatcata aatgttatta accatcatga
     8101 cagcatcacc aatttagtcc ttctttacta aacctcaaca ttaaaatgtg ttttcaacag
     8161 ttttaaagac tgaaccgtat aatgcctaat ttgtaacccc attttaaagt cagtataagt
     8221 ttctggcata tcgataaatg tattaaacat tgctgtaacc ccttgtgatt caaataaatc
     8281 aaacttttca gcagttgcac aaattgcaat ggcaaccttt tgatgtttat gacaaagttc
     8341 tgcaatacgc aatgttgtcg tttctagcaa ctgatcattt tcatttaatc cttctccaaa
     8401 aataattaaa tccgcttgtt caactaaatt ttctaaatgt gttagttggt ctactaatgc
     8461 atgactggtt aatatttcag cttgatacag tccattcaag actgctgcaa ttccaccacc
     8521 agcaccacca cgttcaactg gaccaattgc aattttcaat tcacttttaa ataactcact
     8581 aaaataccaa attaaattat cgatttctgc tgcttgatta tgattcaact gatacgcatc
     8641 ataagtttgc atgatttcac tttgcttacc atataatcga cttgaaaaat ccgacattac
     8701 ttgaattctt gctgtttcca ttttagggtg taagttcgac atatccatac gacgaatata
     8761 tttaattaca ccagcacctt gtctcatatc tacgacacgc ccttcgtcat catagaattg
     8821 agcacctaat gcttgtaaca tacctgcacc agcatcaaaa ctatcaatcc cacctagtga
     8881 aattacaaca tgttttgcgt cgttatctaa tgcatgttta atcatttctc ctaaaccata
     8941 acttgttcgt tcaacaattg gttttttacc ttttaaaaat aaattcccct cgataacggt
     9001 catccctgta tcagtttgtc cgtaaacacc ttcaacttca ttcatatctg catcatgtac
     9061 tggtatacga tacttttgcc cagattgcca taaaaataca gaatctaata attcatgtct
     9121 tccattaaac aatggtactt gaactacatc tgcagtttca atttggcttg caactgcctc
     9181 ttcaacatat ctattagctt gataacttga aataattcca tgaaactcat ccatggctac
     9241 taatactttc ataaccttga ccaccttact ttattatttg ttgtgatacg ttttttattc
     9301 ataaaagatt aatcttaaat tttagatttt atctcgtgat tatatgcgaa catttaacat
     9361 gacatgatta ttaattattc cagtaaataa caatatgctt cttttggttt agcatatatt
     9421 tcctattaaa ctaacttaca aatcatgttt aaattttctt attggattcg tcgttttcca
     9481 aaatattttc aataaaaata ctcgaatata ttatacatat gtttgtaaaa aagtactaac
     9541 ttaactgctt ctaaaggtga cataaaataa aaggtcagaa catcattata gccaaactac
     9601 tctgattcac acgaatttaa gttgtgctat tcacgtctag tagtgtaagg caaaatgttc
     9661 caaccctata gtttcattat tcttctgctt gtgctaatga cgacacattg atatcaccgt
     9721 ggtctcgatt ccaaaccatt tcaccattta caaaataaaa tgcttgaggt gattcatgtt
     9781 taacgttcgt ttttttagca atataatctg acaaatcgcg ttcttgttgg acaatcaaat
     9841 aataaccgtc catatcgcgt tcatataaaa atttattaaa ttgatcgtac gcatttgccg
     9901 atattggaca agtttcacta tgttttaata caaaaacata tttattttcc tcaataacct
     9961 gttcaaattg gtcaattgaa cttagcttta tagccactct attcacctct aaaaatattt
    10021 atcacattta aatagcatct gcgtgacatt tttatacata tagttaatat caatgtttac
    10081 cattatacac atagtaagtt gacatgttaa ccataaattc aaaattttta actttgaaaa
    10141 atgattatag ttatttttct tctaattttt caagcttttt ttcaaaggct ttatcgccgt
    10201 gagttatatc tatacccttt ttcggcaact tatctacatc gacttttgat aacttctcac
    10261 taacctttat tgtttcaata cgtgtattat aatagttctg cagactgtaa tacatttcta
    10321 ttgcgttttt ccttgcatta ttaacatgct tatcactaat attagtttgg ttaatatcgg
    10381 taatttgctt ttcaatcatt ggcataatgt gatttttaat cgcgcctttt acttcatttt
    10441 ctttactatc atctaggttc ttcttcgcag tatctcttaa cgctttatta ttatgttcta
    10501 attttgtcgt aagattagtt gcttcacttt tattatcagc taatttaatt tctgattcaa
    10561 ctttgtatct atttttttca aattgtttcg tataatctaa aatgtcttcg ttatacttga
    10621 tagattgatt acataaacct atgaattttt ttaattgata tatcgcatcc ttcttctcat
    10681 ttgcaagcgt catatattct ttttttaatt tcttaacttt cattgtatca tcaggcaaat
    10741 tttttgctga cttataatat gcttcaaatt taggtatgag atggttttta acatcttctt
    10801 gtagtgcctt aaattcttta ctatttttat cagttgtatc agttttactt agatgatcaa
    10861 tttctttcaa atgtatatta tccatgacat gttgtaattc tttttgtttt tgctcaacat
    10921 ctttaaattg cttagtaaat tgatgttgtg cctctttttc tttattacca caagcactta
    10981 ttactacaaa taaaatcgct aaaacagcga taataacaat ttttttcatg attgcactcc
    11041 ttaacagatt cattatacaa aaacatgttt attatgaaaa tgaattatgt tatagtattc
    11101 cagactttat cgtgattaaa ttaattttca atatcaaagg agtttatcat gtattcaaat
    11161 gttcaaccat tcattaatag tgatttaact aattgttttc aatcacaata cactgaaatt
    11221 gaatcattat tattcaacct cttagacagc ccagtaaaat atacaaaaca tattggcggt
    11281 acgcatcatt ttaaatattc aacagagcca attttagata ttttagtagg tgtagaaaat
    11341 ttgcatgata ttacatcctt agatgaaaaa cgcttaaatt atgtagggtt ttatcgtttg
    11401 catcataaat atactaagaa agttatgatg gcgaagttta acaattttca tgaactaaaa
    11461 caacaggtgc gtctacatat cattcaaatg aatacccaaa catttgaatc ttatcttgca
    11521 cttgattacc tcttatcaac taatgatgat attgctttgc aattcaatac aaaaaaacac
    11581 caaatattgc aacatagctt aacaattaga cagtatgaga tgcaaaagca acaatacttt
    11641 gaaaaactat gtaaaaaata cttataacga caataatgcc gtattttata attatactaa
    11701 ttctaatact ttcttttcaa ttttcgcaaa tgaattttaa aattggtata atactatatg
    11761 atattaaaga catgagaaag gatgtactga gaagtgataa ataaagacat ctatcaagct
    11821 ttacaacaac ttatcccaaa tgaaaaaatt aaagttgatg aacctttaaa acgatacact
    11881 tatactaaaa caggtggtaa tgccgacttt tacattaccc ctactaaaaa tgaagaagta
    11941 caagcagttg ttaaatatgc ctatcaaaat gagattcctg ttacatattt aggaaatggc
    12001 tcaaatatta ttatccgtga aggtggtatt cgcggtattg taattagttt attatcacta
    12061 gatcatatcg aagtatctga tgatgcgata atagccggta gcggcgctgc aattattgat
    12121 gtctcacgtg ttgctcgtga ttacgcactt actggccttg aatttgcatg tggtattcca
    12181 ggttcaattg gtggtgcagt gtatatgaat gctggcgctt atggtggcga agttaaagat
    12241 tgtatagact atgcgctttg cgtaaacgaa caaggctcgt taattaaact tacaacaaaa
    12301 gaattagagt tagattatcg taatagcatt attcaaaaag aacacttagt tgtattagaa
    12361 gctgcattta ctttagctcc tggtaaaatg actgaaatac aagctaaaat ggatgattta
    12421 acagaacgta gagaatctaa acaaccttta gagtatcctt catgtggtag tgtattccaa
    12481 agaccgcctg gtcattttgc aggtaaattg atacaagatt ctaatttgca aggtcaccgt
    12541 attggcggcg ttgaagtttc aaccaaacac gctggtttta tggtaaatgt agacaatgga
    12601 actgctacag attatgaaaa ccttattcat tatgtacaaa agaccgtcaa agaaaaattt
    12661 ggcattgaat taaatcgtga agttcgcatt attggtgaac atccaaagga atcgtaagtt
    12721 aaggagcttt gtctatgcct aaagtttatg gttcattaat cgatactgaa acacgctgtc
    12781 gccattattt taccgaagaa gatattattg ctattaaatt taaatgttgt aataaatact
    12841 atccatgcta taagtgccat aatgagtttg aaaagcacgc cattaagcgt tggtctgagc
    12901 cttcatttaa cgaaaaagca atcttatgcg gtgtatgcaa acacgaatta acaatcaatg
    12961 aatatatgat ggtagagcgt tgtccaaatt gccaatctcg gtttaacaat cgctgtaaat
    13021 atcactatca tatttatttt gaaatttaaa caacatagtc aaaacattat agggagtggg
    13081 tcagacatga taaagcacca cttctgtaga attccttttt gaaattctct atgtttgggc
    13141 cccgccaact tgcattgtca gtagaaattg aaaatcaaat ttctctatgt ttggatccca
    13201 ctagtattga aatgaacttg ttacaagtac atgttcattc aaccaacgac tgacaacaga
    13261 acattttaga gtctaggaca ttgattgacg tcccagattt ccttttttaa agaaaagagg
    13321 ggagcaatga ttaagacctt taaccattgt tcccctcttt taaaattatt ttacaacttt
    13381 atcaagttcc tcaatttgtt taattgtagt tgtagttgat ccagctgcaa agtaccataa
    13441 tttaggatct aaattatata ctttgtcttc tttaattgct ttaacatttt ttaatacagg
    13501 attatttaat gcttgtttcg cagttgattt accacttatc gcttgacctc tatccatcgc
    13561 taaaataaca tctggatttt ctttattaac atattcgtta gaaacatttt gtccatgatt
    13621 gctattactt acttttttat caactgcatt gaatcctaat gtatcgtaaa ctaatccacc
    13681 aaaacgacct ttaggtccaa atgttgataa ttcaccttcg ttaactagta aatacataac
    13741 agttttattg aagtttttcg ttttatcttt cattgaagca attttgttat ctaaatcttt
    13801 atttaattct ttagctttat cttctttatc gtaaattttt ccgatatttt cagtgttttg
    13861 tttcattgaa ccaattaagt tcttttcatc tgcaccaaca taaacaattt tcgctttagg
    13921 tgcagctttt ttgaattcat ctaaattctt ttgattagct gtacgtccag agataaagat
    13981 tacttcgggt ttcgtcgcag caattttatc aaaattcact tcttttaaat taccaacgtt
    14041 tgtatattta tcatctttaa atgattctaa gaaattcggt aatgacttac cgccttcccc
    14101 tttaggtaat gcttttactt tatctgataa gcccatttct ttcattacat ctaatgcgcc
    14161 atagtctaac acaactgcat ttttaggatt ttttggtact tcaacagttt ctttaacttt
    14221 tttagcgtca ctaccatctt ttttctcgcc acgcatttta tagttatttt caatttttac
    14281 tgtatcttta gaacctttag tttctgattt tgattgttct ttatcagaat tgttaccgca
    14341 tgccgctaat aaaaacatta ctgctaatac taaatataag actgttttct tcataatata
    14401 atattctcct cttttggctt aatctgtgtg cttgatgtga aatataaata aatgctttac
    14461 gtttaaccct tctttaattg gcgcatcacc cctttaaagt gtctccaaat tatcaatcct
    14521 taaaatataa acaaatcctt tgatttctta tatgttcaat ttgaatattc atgtcatata
    14581 aatcacttaa aacactactt tgaatgacat tatctttcaa atctgactta actagttgtc
    14641 cgtttttcaa tgctacaatc tgatctgaat aacaggacgc aaagttaata tcatgtaaca
    14701 caatgacaat cgctttattc attttatgcg ctaacatttt taacgtttgc ataatttgaa
    14761 cagcatgctt catatctaaa ttatttaatg gttcatctag caaaatatat tcagtatctt
    14821 gtgctattgt cattgcaatg tatgcacgct gacgttgtcc accagataat gacttaatat
    14881 tacgatttct gatatcttgt agttgcaaca aatctagcgc atcattgaca atatcatgat
    14941 cctctttcgt caaacgacct ttagaataag ggaatcgtcc aaagtttacc aactgctcta
    15001 ccgtaatatt catttcagta tggtttgttt gttttaatat agatattttt ttcgacaagt
    15061 cattattttt ataatcagac atgagctgtc catctatttt cacttcaccg ttctcaaaac
    15121 gaattaacct acatatcgct gaaagtaaag tactcttacc cgcaccatta ggtccaatta
    15181 aagaagtcaa tttacctttt tcgatatcta tgctaatatc ttccaatatc atttgattat
    15241 ttatagtttt agttaaattt tcaacttgaa tcattgcgca tttctccttc taactaatag
    15301 ataaatgaaa taacttccac caatcaaatc aataagtata ctcatttctg tcgtagcttc
    15361 aaacacattt tcaactaccc attgcgcact aaataaacta atccaactca agcaaattgt
    15421 cgcaattaaa atatacttat gttcatacgt cttcattagt tcatgcgcta gatttacagt
    15481 taataaacct aaaaatgtaa ttggtcctac taatgcagtt gacacagaaa ctaaaactga
    15541 gactattaca agtagaattc gcgttaattt ttcatacgat atcccaagat taattgcttc
    15601 agcacgacct aaaagcaata catctaaata aggcaatagt aaaattgtaa tgactaataa
    15661 gattactaat agcactgctg agaaagtaac taaattcgaa ttagaagcat taaaattagc
    15721 aaacatacta ctttgtattg ctaaaaatga ctcaggatcc ataatcagtt gaataaaacc
    15781 tgttatgctt ctaaaaaatg ttcctaaaag gacaccaata agtaagataa aataaattga
    15841 aaaatgtccg attttaaaga taccttggaa taggattagt gcgaataaca ccatcgttat
    15901 aagtgttaat ataaagttca aatatacatt agtaacccat accgattgaa ttccaaataa
    15961 aaagactggc aatactttga taaataaata aactgcatct aaccccatta ttgatggtgt
    16021 caatagacgg ttatttgtaa tcgcttgaaa aatcaccact gaagttgcaa tggcagcacc
    16081 tactaaaata attaatatga actttcttaa acgacttgaa aattgatatt caaatatttc
    16141 aaaatcaata cctacaaata agtacagcat actaattaat aacgttactg caattaaaag
    16201 cattattttt ttattattgt tgcgcataat ttttcctacc tttcataagc aagataagga
    16261 aaatgattgt tccaaataca cctattgtta aaccaatatt tatttcatat ggataaacaa
    16321 caattctgcc aactatatca gaaaataata caaagatggc acctaacatc atcgtatgag
    16381 ggatagcatt tttcaaatga tcacctcgat aaattgaaat aatatttggt attactagtc
    16441 ctaagaacgg taatgttcca acagtcacca ctaccaatgc tgtaatagtt gcagtaataa
    16501 acaatgcgat gttaattaat ttttcgtaac tcaaacctaa attattagta aagtctttac
    16561 ccattcctac aatcgtgaaa tgattagcaa aaagatatgt caatgctaaa agaggaatac
    16621 ttaaatataa aatttcatag cgtccacttg tgataatggc aaagttcccg ttaagccagt
    16681 taccaatgct ttgaacagca ttcgtgcgca atgagatgaa ggttgtgaaa cttgcaacaa
    16741 tcccacccat cataattcct aaaagcggta caaaaatgac atctttcact tttataaaat
    16801 caatgatttt aacaaataaa aacgtaccgc aaatactgca aataacagca aatactagtt
    16861 ttaataaaat atgaccggtt ggaaagaaca ataaagcaat taaaatacct agtttagccc
    16921 attccatcgt tccagctgta gtcggactaa caaacttatt ttgcatcatt tgttgcatta
    16981 tcaagcctgc taaagccaac gaacttccag caattaaaat actcatcgtc cttggtattc
    17041 ggcttgagaa taaaatattc cgctgtgact cagttaaatg tagtaaatct ttaattgata
    17101 gttcactcac acctatgaac aacgaaataa ttgtcaacaa tattagtaat agaagcaaaa
    17161 tatttccctt taataaatac ttcattacaa atttttctcc ctttaaatta atgataatca
    17221 ttatcattga taatatttct cattaatagt agcgtttttt gaccataatt tcaatagcct
    17281 ttcaaataat ttagtgtcaa ttttattgat atttgcaatt aaactgaaat ttaatttttc
    17341 ggaatgtgta ttttgtttac ttaaagtaaa atagaacact taagattttg atgtctggga
    17401 atagtggaaa tgataaaaac tactaatgat tgattaagta gtggttctta acattagcct
    17461 cagctaatgt gtacttaaaa ataggaatac atgagtaaaa ctcatgtgga agtgataatt
    17521 tctaaaggaa aaagtttttc tttatgttgg ggccccgccc gaacttgcac aatattgtta
    17581 gctgactttc cgcctgcttt tatgttgggg ccccgccaac ttgcacaata ttgttagctg
    17641 actttccgcc tgcttctatg ttggggcccc gccaacttgc acaatattgt tagctgactt
    17701 tccgcctgct tctgtgttgg ggccccgcca actactgcca atataaatag cattgtacat
    17761 cttagagcat tgatttctta ctcagactca taatgagtta gatacacata aaaaaagacc
    17821 ttcaacatgt aggagacaag acgaaacgaa tcgctatttc cctttacatg tgaaggtctt
    17881 ttaatttaat tgttatttgt tgtcaaatac aaagtcatca tcttgtaatg cttctacgtt
    17941 taatgctaat acataaccat caccttttac tgagaagaag tcatggtttt tagttgttgt
    18001 atctaaggca ttctcaatga ttgggttaaa ttcacgttcc tcaaaataag gttcaaagcc
    18061 taagtttgaa agtgctttgt ttccattata tttaacatag tttagcacat cttcagtgat
    18121 tccaagatca tcgtataaca tttttgtgta tgactcttca tttaaataca agtcatttag
    18181 caatttatac atttcttgat ctgctttttg tttctcactt tcagatagtt catttcgtaa
    18241 atgctgtgca tctaaaccgg taaatacacc atgaatagat tcatctaaaa gaattttacg
    18301 aatgatttca cctgatgtcg tcattttccc ttgaccagca agatatagtg gataatagaa
    18361 acctgagaag aataagaatg tttctaaaaa tacactcgta actctggcca tatattggtc
    18421 gtatatcgaa gcttctttac cccaaagttt gtgataatta gcaacaattt tatcagattt
    18481 atattttaaa tggggttcct ctaaaaccca ttcatctaat aggtagtttg tttcactaga
    18541 tggtaatagt gttgtgaaaa tatgtgaata gcttttcgcg tgtatttgct ccatcatcgc
    18601 cataaatgaa taaactgctt ttttccttaa gtcagtcgta tgtagcataa ctaaaggcat
    18661 gccatcatct gcttgatgtg tatctaagcc tgttaaacca gctaatgctt ttttaaatgt
    18721 gtcttgttca gcttcagata aagtcttcca acttgcaatg tcttttgata ctttaaattc
    18781 tgtttcaacc cacatttgag atatattttg tctccaaaac atattcgtca tatcttcttg
    18841 tgtgttccaa ttaacagcta tcatgaatat aatttcctcc tgtaatgtta ctcaataatt
    18901 gacaaataca gacatagcag tctaatcatc tgctaattca gagcagatga ttagctgttt
    18961 gtcattttca acatttaatt gttagataga acaacttgta cattcttcta cacttaataa
    19021 tttatttcta gtatagtaaa gtgattttaa tcctttatag tgcgcatata catataatct
    19081 tgctaactca cgtgtagaaa tttcagaatt aacataaagg atcgttgaga taccttggtc
    19141 aatatgcgtt tgaattgtcg caattaaatc gattaatttc atctgatcag tattgaatgc
    19201 tgatttgtag taccacattg tttgtggtga taagaatggc atagggtaaa atgtttccgc
    19261 attaccataa gtacgacgtt caatttggtc aacgattggc attacagaac ttgttgcatt
    19321 ttgaacataa gaaatacttt gtgttggagc aattgctaat ctatatgcat gatataaacc
    19381 atattgttca acatcttgtt gtagtttctt ccaatcctca gaagtaggaa tagccatacc
    19441 atcgaataat tcacgtactt tttcgaattg aggttcaaat tcttgagttg tatagaactc
    19501 gaaatatttg ccattagcat aatcagactt ttcaaagtct tgatatttga taccacgctc
    19561 tttagcgatt tccattgaac gttcgattga gtagaaattc atcatcataa agaagatatt
    19621 tgcaaaatct tttgcttctt ctgactcata accaatttta ttttttgcta ggtaaccgtg
    19681 taaattcatc acaccaagac caactgaatg taattcactg ttagcttttc taactcctgg
    19741 tgcattttga atatttgcta catcactcac aacagttaat gcgtccatac cagagtgaac
    19801 tgaatctctg aattttccgc tttccattac attaacaata tttaatgagc ccaagttaca
    19861 agaaatatca cgtttaattt cgtcttcaat accatagtca ttaataattg aagtttcttg
    19921 tagttggaaa atttccgtac ataagttact cattttaatt tgtccaatgt ttgaattcgg
    19981 atgcactctg ttagcattat ctttaaacat taaatatgga taacctgatt gtaattgtgt
    20041 ttgcgcaatt aaattcaaca tttcacgcgc attctttttc tttttctcaa catttgggtt
    20101 tgcaaccatg tcatcataat atttttctaa atcgatatcg tctaatgtca caccatattc
    20161 ttctttaact gtatgaggtg caaacatata aaagtcctta ccttctttag ctaaatcgaa
    20221 gaatttagat ggaacaatta aaccagttga tattgtagat aaacgtaaat cttcatccgc
    20281 atttactttt ttagtatcta aaaattcttc tacatcataa tggaagatat ttaagtacac
    20341 agcaccagca ccagggcgtt gaccaagttg atctgcatag ctaaagccac cttcaagtga
    20401 cttagcaata ggtaaaacgc ctttcgctac gcctttaatt cctttaattg cttcaccacg
    20461 tgcacgcaat ttagataagt taattgcaac gccgccccca attttactta attgttttgc
    20521 agttgaatca ataaagttaa ttgaatttaa gctgtcatcc acttctaata agaaacatga
    20581 cactagctca ccacgacgcg cacggcctgc gtttaaaaat gttggtgtcg ctggttgata
    20641 tctttgttca accatagcag aaataaattg tttagcttgt gctttattac catttgctag
    20701 gtataaagca acaatggcaa cgtgttgatt atagtcttct aagtattgac ttttatcatt
    20761 tgttttcaaa gcgtaatctt tgaaaaattt actagctgac atataacttg caaaattaaa
    20821 cgggattgat tttgcataat cagtgatttc aattagatcc gcttcactat aaatatcaaa
    20881 cacattgaaa taaaaatcgt tgtctactaa ataacgtaaa cgctcgattt cagtgtcgaa
    20941 gaagattgtt ttgtctttta cttcttctaa ataagctact aaagcttctt ggtctttttc
    21001 taaactaaag aatccatctt ctcttcgttt agtgacctca ttatttaatt caatatgatt
    21061 gtactttttc tcttccatgg ttttcattaa aattacccac cttgttctta aattcaataa
    21121 cgtctttgtt ttttccatgt aactcaaact tcattaataa agggacatta tactcttctg
    21181 atatcgtgcg acccgctttt gcgaaattta gtccccaatt tcgattaccg ctagctgcca
    21241 cacctctgat gtattgatga ttaacttcta aaaaagattg aacgggttct ggtacttctc
    21301 caaatccaat agtgccagta acgataataa acggttcatg aactggttcc atacaatttt
    21361 ctgctgtaat ctcaagcgta ttttcaagtt ctgttctctt aataaaacga cggacatttc
    21421 cagtaaatga aaaatatatt attttcattg gatcaccttc tttcttcaac ccttttaaat
    21481 aaagtagtca ttccaattaa aaaatgcttt aacaacatgg ttactatggt tatctcagca
    21541 ttgtattaac tataatgaag ctacttattt tctcaaatgt aaaccaattg catagattta
    21601 tatttgagaa aatagtagct tatatgggtt gaatgtataa aaatcactat atgtagtgtt
    21661 tacatcattg gatatcttcc atttgctcag tacattttct aaaaatatga ctattaattg
    21721 ataataacta ttaaatttct gtgttaaatt acttttaaat gtaatttctt cataaataaa
    21781 gcatttgcat acaaaacaca agatattgtg tctttttcgt ttcaccaaca ctatattatg
    21841 tgtttcatta tacataagac acactatgat ttaaagtgcc attttctaat cgaatgttcg
    21901 taaaacgttc caactaggca ccaatactaa attagaaaaa cttgaacaaa tttaaaaaaa
    21961 ttttttagta gatatcactt gattttttta aatctgtgtc aatctcaatt gtatcaacag
    22021 aaatcaagaa aaagcttatt agcttgatac tgaattgata tttgttttat gtaataacac
    22081 aaacattcat aaaagtgtaa tgccctatta ttccgacaaa taaacacaga acgtttgttc
    22141 gctacagtct attatagtct ttttatagta aatttcaact atttatgcac aacatgttgt
    22201 tttatgctaa tataaatacg ttaattttat ataaaagagg cgataaaatt gaatcctaaa
    22261 gtaaaaggta tcattgccat tttaatttcg gctattggtt tcagttttat gtcagtgttt
    22321 ttcagacttg caggtgacct tcctgttttt caaaaatcat tagcacgaaa cttagtagca
    22381 atgtttattc cattattttt tatatataaa taccatcagc caatgtttgg cagactttca
    22441 agtcaaccct tattaataac acgatcaaca cttggactta ttggtgtatt gttaaatatt
    22501 tatgccattg atcatatggt acttagtgat gccgattcat taatgaaact caatcctttc
    22561 tggacgattt tattaagcat tgttttctta catgaaaagg taaggaagta tcaaattaca
    22621 gcaatgataa ttgctatttt aggtatgctt ttaatagtga aacctgaatt ttcatcatct
    22681 atgattccat cattagctgg cttattctca ggtatttttg cagcatcagc atatacatgt
    22741 gttcgagcac ttagtacaag agaagcacca tatactatag tattttactt ctccttattt
    22801 tctgtcatcg ttttaatacc tttcactgca tatacttatg aaccaatgag tcagatgcaa
    22861 atactttatc tattaggggc tggcttagct gccgctgtgg gtcaaattgg tgttacttta
    22921 gcatatagtt ttgcagcagc aaaagatatt tctattttta cgtatgcatc tataattttc
    22981 acagcaatac ttggatttat tctattcgga gaatctccag atttctatgc aacattaggt
    23041 tacgtcgtta ttatcggtgc aagttattac atgtttgaaa aagcacgccg agacgccaaa
    23101 ataataaaaa aataaattta aaggagcgct ttatatggca catggccgtc aacaagatga
    23161 attacaagat ataactttat taggaaatca agacaatact tacaattttg actatcgtcc
    23221 tgatgtatta gaatcgtttg ataataagca tcaaggtcgt gactatttcg taaaattcaa
    23281 ctgtccagaa tttacatcat tatgtccaat tactgggcaa ccagattttg caactatcta
    23341 tatttcatat attccaaatg ttaaaatggt tgaatcaaaa tctttgaa
//
LOCUS       DAKTIO010000019        23096 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.019, whole genome shotgun sequence.
ACCESSION   DAKTIO010000019 DAKTIO010000000
VERSION     DAKTIO010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 23096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23096
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.019"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            107..286
                     /gene="prli42"
                     /locus_tag="O0293_000606"
     CDS             107..286
                     /gene="prli42"
                     /locus_tag="O0293_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stressosome-associated protein Prli42"
                     /protein_id="HCY0279505.1"
                     /translation="MLVISSQVKSFISYVVIINTKVIHLEAHKMQNKVLRIIIIVMLV
                     SVVLALLLTSIIPIL"
     gene            complement(413..1393)
                     /locus_tag="O0293_000607"
     CDS             complement(413..1393)
                     /locus_tag="O0293_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCY0279506.1"
                     /translation="MLKLNPYKIGFRTIKTAVGMTLGVIISKLLGLDNYASSAILVVL
                     CIKHTKVHSLQAIISRLVSCFLVLFLGSAIFSLLGQSPIVLGIIVLLFIPLTVVLKVQ
                     EGVITSCVILLHVFNAKSIDAHLIVNETLLLLIGLSIAFTMNLMMPSLDKQLDEYKCK
                     IEQQIADIFSKYSYICEKYEDTIAIEFEVLLLNIKKAKSIAFRDVKNHFVRNENSYYH
                     YFDMREEQVELLMRMKPLIESICHKDPLLNDVASLLKEIGLNVNSNDYTAMRLHNLYE
                     TRLKLDELPLPTTHKALNSRASIIQILNELEEYLQIKSQFGSLKMHSEVA"
     gene            complement(1407..1844)
                     /locus_tag="O0293_000608"
     CDS             complement(1407..1844)
                     /locus_tag="O0293_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HCY0279507.1"
                     /translation="MDMNFDLYMNGVVEQARNEIESAGYEQLTTAEDVDKVLKQDGTT
                     LVMINSVCGCAGGIARPAASHALHYDVLPDRLVTVFAGQDKEATQRAREYFEGYAPSS
                     PSFALVKDGKITEMIERHQIEGHDVMNVINQLQTLFNKYCEER"
     gene            complement(2195..3469)
                     /locus_tag="O0293_000609"
     CDS             complement(2195..3469)
                     /locus_tag="O0293_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HCY0279508.1"
                     /translation="MEITMPKLGESVHEGTIEQWLVSVGDHIDEYEPLCEVITDKVTA
                     EVPSTISGTITEILVEAGQTVAIDTIICKIETADEKTNETTEEIQAKVDEHTQKSTKK
                     ASATVEQTSTAKQNQPRNNGRFSPVVFKLASEHDIDLSQVVGSGFEGRVTKKDIMSVI
                     ENGGTTAQSDKQVQTKSTSVDTSSNQSSEDNSENSTIPVNGVRKAIAQNMVNSVTEIP
                     HAWMMIEVDATNLVNTRNHYKNSFKNKEGYNLTFFAFFVKAVADALKAYPLLNSSWQG
                     NEIVLHKDINISIAVADENKLYVPVIKHADEKSIKGIAREINTLATKARNKQLTAEDM
                     QGGTFTVNNTGTFGSVSSMGIINHPQAAILQVESIIKKPVVINDMIAIRNMVNLCISI
                     DHRILDGLQTGKFMNHIKQRIEQYTLENTNIY"
     gene            complement(3482..4465)
                     /locus_tag="O0293_000610"
     CDS             complement(3482..4465)
                     /locus_tag="O0293_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCY0279509.1"
                     /translation="MAKLSYLEAIRQAQDLALQQNKDVFILGEDVGRKGGVFGTTQGL
                     QQKYGEDRVIDTPLAESNIVGTAIGAAMVGKRPIAEIQFADFILPATNQIISEAAKMR
                     YRSNNDWQCPLTIRAPFGGGVHGGLYHSQSIESIFASSPGLTIVIPSTPYDAKGLLLS
                     SIESNDPVLYFEHKKAYRFLKEEVPEEYYTVPLGKADVKREGEDLTVFCYGLMVNYCL
                     QAADILAADGINVEVVDLRTVYPLDKETIIDRAKHTGKVLLVTEDNLEGSIMSEVSAI
                     IAEHCLFDLDAPIMRLAAPDVPSMPFSPVLENEIMMNPEKILNKMRELAEF"
     gene            complement(4465..5457)
                     /locus_tag="O0293_000611"
     CDS             complement(4465..5457)
                     /locus_tag="O0293_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HCY0279510.1"
                     /translation="MIDYKSLGLSEEDLKVIYKWMDLGRKIDERLWLLNRAGKIPFVV
                     SGQGQEATQIGMAYALEEGDITAPYYRDLAFVTYMGISAYDTFLSAFGKKDDVNSGGK
                     QMPSHFSSRAKNILSQSSPVATQIPHAVGAALALKMDGKKKIATATVGEGSSNQGDFH
                     EGLNFAGVHKLPFVCVIINNKYAISVPDSLQYAAEKLSDRALGYGIHGEQVDGNDPLA
                     MYKAMKEARDRAISGQGSTLIEAVTSRMTAHSSDDDDQYRTKEERETLKKADCNEKFK
                     KELLSAGIIDDAWLAEIEAEHKDIINKATKAAEDAPYPSVEEAYAFVYEEGSLN"
     gene            complement(5473..6894)
                     /gene="lpdA"
                     /locus_tag="O0293_000612"
     CDS             complement(5473..6894)
                     /gene="lpdA"
                     /locus_tag="O0293_000612"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCY0279511.1"
                     /translation="MSEKQYDLVVLGGGTAGYVAAIRASQLGKKVAIVERQLLGGTCL
                     HKGCIPTKSLLKSAEVFQTVKQAAMFGVDVKDANVNFENMLARKEDIINQMYQGVKHL
                     MQHNHIDIYNGTGRILGTSIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPF
                     LSFDHDKILSSDDILSLKTLPSSIGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILP
                     TESKQASQLLKKSLSARGVKFYEGIKLSENDINVNEDGVTFEISSDIIKVDKVLLSIG
                     RKPNTSDIGLNNTKIKLSTSGHILTNEFQQTEDKHIYAAGDCIGKLQLAHVGSKEGVV
                     AVDHMFEGNPIPVNYNMMPKCIYSQPEIASIGLNIEQAKAEGMKVKSFKVPFKAIGKA
                     VIDSHDTNEGYSEMVIDQSTEEIVGINMIGPHVTELINEASLLQFMNGSALELGLTTH
                     AHPSISEVLMELGLKAESRAIHV"
     gene            complement(7045..8724)
                     /gene="recN"
                     /locus_tag="O0293_000613"
     CDS             complement(7045..8724)
                     /gene="recN"
                     /locus_tag="O0293_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCY0279512.1"
                     /translation="MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQ
                     LIGMRASSDFVRHGEKKAVIEGIFDIDESKDAIHILKNMDIDVDEDFLLVKREIFSSG
                     KSLCKINNQTVTLQDLRKVMQELLDIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLD
                     KYHQTFQNYKAKKQELEDLESADQALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIK
                     RIQNSEKLSLALNNAHMTLTDENAITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFY
                     YILEDAKHELYDEMANTEFDEQVLNEYESRMNLLNNLKRKYGKDISELIAYQEKLNNE
                     INKIENYEQSTSQLREEINALYNQVIEVGQALSKQRRIVARELRDHIVSEIQNLQMKD
                     ANLEISFKKLEEPNIDGIEFVEFLISPNKGEPLKSLNKIASGGELSRIMLALKSIFVK
                     SRGQTAILFDEVDSGVSGQAAQKMAEKMRDIAEYIQVICISHLPQVASMSDHHLLISK
                     SSKDDRTTTQVQELISDDKVDEIARMISGASVTDLTRENAREMIQHNQRRR"
     gene            complement(8740..9192)
                     /gene="argR"
                     /locus_tag="O0293_000614"
     CDS             complement(8740..9192)
                     /gene="argR"
                     /locus_tag="O0293_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCY0279513.1"
                     /translation="MPKKSVRHIKIREIISNEQIETQDELVKRLNDYDLNVTQATVSR
                     DIKELQLIKVPIPSGQYVYSLPNDRKFHPLEKLGRYLMDSFVNIDGTDNLLVLKTLPG
                     NAQSIGAILDQINWEEVLGTICGDDTCLIICRSKEASDEIKSRIFNLL"
     gene            complement(9624..10505)
                     /locus_tag="O0293_000615"
     CDS             complement(9624..10505)
                     /locus_tag="O0293_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCY0279514.1"
                     /translation="MTNLPMNKLIDEVNNELSVAINKSVMDTQLEESMLYSLNAGGKR
                     IRPVLLLLTLDSLNTEYELGMKSAIALEMIHTYSLIHDDLPAMDNDDYRRGKLTNHKV
                     YGEWTAILAGDALLTKAFELISSDDRLTDEVKIKVLQRLSIASGHVGMVGGQMLDMQS
                     EGQPIDLETLEMIHKTKTGALLTFAVMSAADIANVDDTTKEHLESYSYHLGMMFQIKD
                     DLLDCYGDEAKLGKKVGSDLENNKSTYVSLLGKDGAEDKLTYHRDAAVDELTQIDEQF
                     NTKHLLEIVDLFYSRDH"
     gene            complement(10483..10713)
                     /locus_tag="O0293_000616"
     CDS             complement(10483..10713)
                     /locus_tag="O0293_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCY0279515.1"
                     /translation="MTKETQSFEEMMQELEQIVQKLDNETVSLEESLDLYQRGMKLSA
                     ACDTTLKNAEKKVNDLIKEEAEDVKNDESTDE"
     gene            complement(10706..12043)
                     /locus_tag="O0293_000617"
     CDS             complement(10706..12043)
                     /locus_tag="O0293_000617"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCY0279516.1"
                     /translation="MSDYLSVSALTKYIKYKFDQDPHLQSVLIKGELSNFKKHSSGHL
                     YFNVKDKESVISAMMFKGSASKLNFEPKEGDEVLLEARVSVFERRGNYQIYVNKMQLD
                     GIGNLYQKLEALKKKLTEEGCFDKANKKSIPKFPKKIAVLTASTGAAIRDIHSTINSR
                     FPLAEQIQISTLVQGEKAKDDIIEKIEYADSLGVDTIIVGRGGGSIEDLWNFNEEAVV
                     RAIYNCKTPIISAVGHETDFTLSDFAADIRAATPTQAAVIATPDQYELLQQIQQYQFT
                     LTRFIKKHLEQQRKHVEHLSSYYKFKQPTLLYDQQIQRRDDLEKRLKQQIQATFEQQR
                     HRLMLLQQRYNLKALLSSVNQEQQNNLQLTNQLVKLLNSKILSYKNDLKNKVENLNNL
                     SPTNTMLRGYAIVNKKDEVITSTKDLTENDQLTLTMKDGLVDAKVTKVRCNND"
     gene            complement(12060..12449)
                     /gene="nusB"
                     /locus_tag="O0293_000618"
     CDS             complement(12060..12449)
                     /gene="nusB"
                     /locus_tag="O0293_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCY0279517.1"
                     /translation="MSRKESRVQAFQTLFQLEMKDSDLTINEAISFIKDDNPDLDFEF
                     IHWLVSGVKGHEPVLDETISPYLKDWTIARLLKTDRIILRMATYEILHSDTPAKVVMN
                     EAVELTKQFSDDDHYKFINGVLSNIKK"
     gene            complement(12509..12871)
                     /locus_tag="O0293_000619"
     CDS             complement(12509..12871)
                     /locus_tag="O0293_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCY0279518.1"
                     /translation="MVKVTDYSNSKLGKVEIAPEVLSVIASIATSEVEGITGHFAELK
                     ETNLEKVSRKNLSRDLKIESKEDGIYIDVYCALKHGVNISKTANKIQTSIFNSISNMT
                     AIEPKQINIHITQIVIEK"
     gene            complement(12886..14241)
                     /gene="accC"
                     /locus_tag="O0293_000620"
     CDS             complement(12886..14241)
                     /gene="accC"
                     /locus_tag="O0293_000620"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCY0279519.1"
                     /translation="MKKVLIANRGEIAVRIIRACRDLGIQTVAIYSEGDKDALHTQIA
                     DEAYCVGPTLSKDSYLNIPNILSIATSTGCDGVHPGYGFLAENADFAELCEACQLKFI
                     GPSYQSIQKMGIKDVAKAEMIKANVPVVPGSDGLMKDVSEAKKIAKKIGYPVIIKATA
                     GGGGKGIRVARDEKELETGFRMTEQEAQTAFGNGGLYMEKFIENFRHIEIQIVGDSYG
                     NVIHLGERDCTIQRRMQKLVEEAPSPILDDETRREMGNAAVRAAKAVNYENAGTIEFI
                     YDLNDNKFYFMEMNTRIQVEHPVTEMVTGIDLVKLQLQVAMGDVLPYKQEDIKLTGHA
                     IEFRINAENPYKNFMPSPGKIEQYLAPGGYGVRIESACYTNYTIPPYYDSMVAKLIIH
                     EPTRDEAIMAGIRALSEFVVLGIDTTIPFHIKLLNNDIFRSGKFNTNFLEQNSIMNDE
                     G"
     gene            complement(14241..14705)
                     /gene="accB"
                     /locus_tag="O0293_000621"
     CDS             complement(14241..14705)
                     /gene="accB"
                     /locus_tag="O0293_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCY0279520.1"
                     /translation="MNFKEIKELIEILDKSTLTEINIEDTKGKVTLKKEKETEIITPQ
                     ISQMPVEAAAMPMPQAQSTDSNKTEAPKPTSDNHKTINAPMVGTFYKSPSPDEEAYVQ
                     VGDTVSNETTVCILEAMKLFNEIQAEISGEIVEILVEDGQMVEYGQPLFKVK"
     gene            complement(15179..15736)
                     /gene="efp"
                     /locus_tag="O0293_000622"
     CDS             complement(15179..15736)
                     /gene="efp"
                     /locus_tag="O0293_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCY0279521.1"
                     /translation="MISVNDFKTGLTISVDNAIWKVIDFQHVKPGKGSAFVRSKLRNL
                     RTGAIQEKTFRAGEKVEPAMIENRRMQYLYADGDNHVFMDNESFEQTELSSDYLKEEL
                     NYLKEGMEVQIQTYEGETIGVELPKTVELTVTETEPGIKGDTATGATKSATVETGYTL
                     NVPLFVNEGDVLIINTGDGSYISRG"
     gene            complement(15762..16823)
                     /locus_tag="O0293_000623"
     CDS             complement(15762..16823)
                     /locus_tag="O0293_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCY0279522.1"
                     /translation="MSRITQVHRILEQKHLDAIIILSDYNRRYLSGFTGTSGALIISK
                     DKQYLITDFRYIDQATKQAPNYEIINRKSTIIGEIKELLHQENFENVGFEGHHVSYDT
                     YLELNKSRISLISISNTVDKIRDVKDADEIALIQKAANIVDETYEYILTVVKAGMTEK
                     ELKAILESKMLELGADGPSFDTIVASGHRGALPHGVASDKIIEKGDMITLDFGAYYNG
                     YCSDITRTFAIGEPDPKLKEIYQIVLESQMKAINEIRPGMTGAEADAISRNYLESKGY
                     GKEFGHSLGHGIGLEIHEGPMLARTIQDKLQVNNCVTVEPGVYIEGLGGIRIEDDILI
                     TENGCQVFTKCTKDLIVLT"
     gene            16928..17509
                     /locus_tag="O0293_000624"
     CDS             16928..17509
                     /locus_tag="O0293_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279523.1"
                     /translation="MKKLVSIVGATLLLAGCGSQNLAPLEEKTTDLREDNHQLKLDIQ
                     ELNQQISDSKSKIKGLEKDKENSKKTASNNTKIKLMNVTSTYYDKVAKALKSYNDIEK
                     DVSKNKGDKNVQSKLNQISNDIQSAHTSYKDAIDGLSLSDDDKKTSKNIDKLNSDLNH
                     AFDDIKNGYQNKDKKQLTKGQQALSKLNLNAKS"
     gene            17523..17741
                     /locus_tag="O0293_000625"
     CDS             17523..17741
                     /locus_tag="O0293_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279524.1"
                     /translation="MRNIIFYLVLIIAAIGLVMNLDAFIFSIVRMLISFAVIAGIIYL
                     IYYFFILTEDQRKYRKAMRKYKRNQRRK"
     gene            complement(17805..18635)
                     /locus_tag="O0293_000626"
     CDS             complement(17805..18635)
                     /locus_tag="O0293_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCY0279525.1"
                     /translation="MTETWNFINTGSKDPYYNMAMDEALLNFVSRGEIDPVIRFYTWN
                     PATLSIGYFQRLQKEIDIDKVKEKGFGLVRRQTGGRGVLHDKELTYSVIVPESHPNMP
                     STVTEAYRVISQGLLEGFKNLGFDTYFAVPKTPEERQKLKQPRSSVCFDAPSWYELVV
                     EGRKIAGSAQTRQKGVILQHGSILQDIDIDELFDMFIYKNERLKLKMKEAFVEKAVAI
                     NDISDEHITISQMEEAFEKGFKKGLNIEFKPLELTEAQLAEVEELTEKYRSDEWMFRK
                     "
     gene            18793..19179
                     /locus_tag="O0293_000627"
     CDS             18793..19179
                     /locus_tag="O0293_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCY0279526.1"
                     /translation="MSASLYIAIILVIAIIAYMIVQQILNKRAVKELDQNEFHNGIRK
                     AQVIDVREKVDYDYGHINGSRNIPMTMFRQRFQGLRKDQPVYLCDANGIASYRAARIL
                     KKNGYTDIYMLKGGYKKWTGKIKSKK"
     gene            complement(19544..21016)
                     /gene="gcvPB"
                     /locus_tag="O0293_000628"
     CDS             complement(19544..21016)
                     /gene="gcvPB"
                     /locus_tag="O0293_000628"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HCY0279527.1"
                     /translation="MTSKSSPLIFERSREGRYAYSLPKSDIKTNSVESLLDDKFIRKN
                     KAEFPEVAELDLVRHYTELSNKNFGVDNGFYPLGSCTMKYNPKINEKVARIPGFSESH
                     PLQDEDQVQGSLEIIYSLQEELKEITGMDEVTLQPAAGAHGEWTALMIFKAYHENNGE
                     GHRDEVIVPDSAHGTNPASASFAGFKSVTVKSNERGEVDIDDLKRVVNENTAAIMLTN
                     PNTLGIFEKNIMEIREIVHNAGGLLYYDGANLNAIMDKVRPGDMGFDAVHLNLHKTFT
                     GPHGGGGPGSGPVGVVKELASYLPKPMVIKDGDKFKYDNDIKNSIGRVKPFYGNFGIY
                     LRAYTYIRTMGATGLKEVSEAAVLNANYIKARLSKHFEIPYKQYCKHEFVLSGVRQKE
                     FGVRTLDMAKRLLDFGVHPPTIYFPLNVEEGMMIEPTETESKETLDYFIDTLISIAEE
                     AKNDPDKVLEAPHTTVIDRLDEATAARKPILKFENLKQEK"
     gene            complement(21009..22355)
                     /gene="gcvPA"
                     /locus_tag="O0293_000629"
     CDS             complement(21009..22355)
                     /gene="gcvPA"
                     /locus_tag="O0293_000629"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HCY0279528.1"
                     /translation="MSHRYIPLTEKDKQEMLQTIGAKSIGELFGDVPSDILLNRDLNI
                     AEGEAETTLLRRLNRIASKNITKETHTSFLGAGVYDHYAPSVVDAMISRSEFYTAYTP
                     YQPEISQGELQAIFEFQTLICELTDMDVANSSMYDGMTSFAEACILAFSQTKKNKIVV
                     SKGLHYQALQVLHTYAKTRKEFEVVEIDLDGTVTDLKKLEAAVDDETAAVAVQYPNFY
                     GSIEDLEKIHSFIEDKKALFIVYANPLALGLLTPPGSFGADIVVGDTQPFGIPAQFGG
                     PHCGYFATTKKLMRKVPGRLVGQTQDDEGNRGFVLTLQAREQHIRRDKATSNICSNQA
                     LNALASSIAMSALGKQGIYDIAVQNIEHANYAKQQFIKKGFEVLDGTSFNEFVVKFDK
                     PIQQVNEELVKYNIIGGFDLGVVSDDFKNHMLIAVTELRTKDEIDTFVEKAGELND"
     gene            complement(22375..>23096)
                     /gene="gcvT"
                     /locus_tag="O0293_000630"
     CDS             complement(22375..>23096)
                     /gene="gcvT"
                     /locus_tag="O0293_000630"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCY0279529.1"
                     /translation="NWILKHKEKFDVEVQNVSNQYGQLAIQGPKARDLINQLVDEDVT
                     EMKMFEFKQGVKLFGANVILSQSGYTGEDGFEIYCNIDDTEKIWDGLLEYNVMPCGLG
                     ARDTLRLEAGLPLHGQDLTESITPYEGGIAFASKPLIDADFIGKSVLKDQKENGAPRR
                     TVGLELLEKGIARTGYEVMDLDGNIIGEVTSGTQSPSSGKSIALAMIKRDEFEMGREL
                     LVQVRKRQLKAKIVKKNQIDK"
BASE COUNT         7122 a         4313 c         3175 g         8486 t
ORIGIN      
        1 aagctttgtc aaaaatatat tgattgattt ttcaaagata tcgtataatg aggaaaatga
       61 cataagcaaa cttactcatg ttttttatta tattccttta tgatgattgc tagttatatc
      121 gtctcaagtt aaaagtttta tatcttatgt cgtaattatt aatacaaagg ttattcattt
      181 ggaggcacac aaaatgcaaa ataaagtttt aagaattatc attatcgtta tgcttgtatc
      241 agttgtatta gcattgttat taacgagtat cattccaatt ttataaacta tatctcaact
      301 acctatacaa aatcatacaa ttaaaaatcc atccattata aacgcatgta ttaataagtt
      361 atcgtattgc aacgattact ttcaaacatg gctcatacgg atggattatt ttttaagcta
      421 cttcactatg cattttcaat gaaccaaatt gcgatttgat ttgtaaatat tcttctaatt
      481 catttaatat ttgaataata cttgctctcg agttaagcgc tttgtgtgtt gttggcaatg
      541 gcagttcatc caatttcaaa cgcgtctcat acaaattgtg taaacgcatt gctgtatagt
      601 cattactatt cacatttaga ccaatttctt tcagcagtga cgcaacatca tttaaaagcg
      661 gatctttatg acagatactt tcgatgagcg gtttcattct cattaacaat tccacttgct
      721 cttctcgcat atcaaaataa tgatagtatg aattttcgtt tctaacaaaa tgatttttaa
      781 catctcggaa cgcgatagac ttcgcctttt taatatttaa aagtaacact tcaaattcaa
      841 tcgcaatggt atcttcatat ttttcacaaa tataactata tttactaaaa atatcagcaa
      901 tttgttgctc aattttacat ttgtattcgt ctagttgttt gtctaaactt ggcatcatta
      961 aattcattgt aaatgcaatg cttagtccaa ttaacagtaa taatgtttca ttaacaatta
     1021 aatgtgcatc aattgatttt gcattaaaaa catgaagtaa tataacgcaa ctcgtaatga
     1081 caccttcttg tacttttaat acgacagtta atggtataaa taacaatacg ataataccga
     1141 gtacaattgg actctgacct aataaactaa atattgctga acctaaaaac aatactaaaa
     1201 aacatgatac taatcttgaa ataatcgctt gtagcgaatg tacttttgta tgtttaatac
     1261 ataatacgac taatatggcg cttgaagcat aattatctaa acctaacagc ttactaataa
     1321 ttacacctaa agtcataccc actgctgttt ttattgttct aaatccaatc ttgtaaggat
     1381 ttaactttaa catgggttag cgcctcttat ctttcttcac aatatttatt gaataatgtt
     1441 tgtaattgat taattacgtt catcacatca tgaccttcga tttgatgtct ttcaatcatt
     1501 tctgtaatct ttccatcttt tactaatgca aatgacggac ttgaaggcgc ataaccttcg
     1561 aagtattcac gcgctctttg tgtcgcttct ttatcttgtc cagcaaatac tgtcactaga
     1621 cgatcaggta atacgtcata atgtaaagca tgtgatgctg ctggtcttgc gataccacct
     1681 gcacaaccac atacagaatt gatcataact agtgttgtac catcttgttt aagaactttg
     1741 tcaacatctt ctgcagtagt taattgctca tatcccgcag attcaatttc attccttgct
     1801 tgttctacaa caccgttcat gtataaatcg aaattcatgt ccataagttc aatcacctat
     1861 ccctttatat ttaaactatc ctcattctac taattaataa catattgttc aataaactaa
     1921 tctgaatcac acctatattt agacacaatt ttaacaatat accaaacatt attgtgctta
     1981 aaatcatggt aactaatttg ttcacatgtt ttcattaata tgtttcaagt atgatgtctt
     2041 attttgactt tactgcaaaa atgcattcaa ccatgttgat tattgttctt tatctttttt
     2101 gaatatattg cacatatttt agtgccaaaa aataatacat ccatcgacaa gaacaagata
     2161 aaacaagtcg tcgatagatg catctatgtt atcactaata tatatttgta ttttctaaag
     2221 tatactgttc gatacgctgt ttaatatgat tcataaattt acctgtttgt aaaccatcta
     2281 aaatacgatg atcaattgaa atacataaat taaccatgtt acgaattgca atcatatcat
     2341 taattactac tggcttttta atgattgatt ctacttgtaa aatcgctgct tgtggatgat
     2401 ttataatacc cattgatgat actgaaccaa atgtaccagt attatttacc gtaaatgtac
     2461 cgccctgcat atcttcagct gtcaattgct tattacgcgc tttcgttgct aaagtattaa
     2521 tttctctagc tatacctttg attgactttt cgtctgcatg cttaatcaca ggtacgtata
     2581 atttattttc atcagcaaca gcaattgaaa tattaatgtc tttatgtaag acaatttcat
     2641 ttccttgcca gctactattt aataaaggat atgcttttaa agcatctgct acagctttta
     2701 caaagaaagc aaagaacgtt agattatatc cttctttatt tttaaagctg tttttataat
     2761 gatttctcgt attcacaaga tttgtagcat ctacttcaat catcatccat gcatgtggaa
     2821 tctctgttac actattaacc atattttgcg caattgcttt acgcacacca tttactggta
     2881 ttgtgctgtt ttcactattg tcttcagatg attggttact tgatgtatct actgatgttg
     2941 attttgtttg aacttgtttg tcagattgag ctgtggtacc accattttca ataactgaca
     3001 ttatatcctt cttagttaca cgaccttcaa atccactacc tacaacttgt gataaatcaa
     3061 tgtcatgctc tgaagcgagt ttaaatacaa caggtgaaaa gcgaccatta ttacgtggtt
     3121 gattttgttt agcagtagat gtctgttcca ctgttgcact agctttttta gtagatttct
     3181 gagtatgctc atccactttt gcttgtatct cttcagttgt ttcatttgtc ttttcatcag
     3241 cagtttcaat tttacagata attgtatcaa tagctactgt ctgccccgct tcaactaaaa
     3301 tttctgtaat tgttcctgat atcgtggaag ggacttcagc tgtcacttta tctgtaataa
     3361 cttcacataa tggttcatat tcatcaatat gatcaccaac agaaactaac cattgttcaa
     3421 tggtgccttc atgaacactc tcacctaact taggcattgt tatttccatg actttccctc
     3481 cctagaattc tgctaattca cgcattttat ttaagatttt ttctggattc atcataattt
     3541 cattttctaa tacaggagaa aatggcatag atggtacatc tggagcagct aaacgcatga
     3601 ttggtgcatc taaatcgaac aagcaatgct ctgcaataat cgctgacact tctgacataa
     3661 tactaccttc taaattatct tcagttacaa gtaaaacttt acctgtatgt ttagcacgat
     3721 caataattgt ttctttatct aatggataaa cagttcgtaa atcaacgact tcaacattga
     3781 taccgtctgc agctaaaata tccgctgctt gtaaacaata attgaccatt aatccataac
     3841 aaaatactgt taaatcttca ccttcacgtt tcacatctgc ttttcctaaa ggtacagtgt
     3901 aatattcttc tggcacttct tcctttaaga aacgataagc ttttttatgc tcaaagtaca
     3961 atactggatc atttgattcg atagatgata ataaaagccc tttagcatca tacggtgtgg
     4021 aaggaataac aattgttaaa cctggcgatg aagcaaatat actttcaata ctttgtgaat
     4081 gatatagtcc tccgtgaaca ccgccaccaa atggtgcacg aatcgttaat gggcattgcc
     4141 aatcattatt tgaacgataa cgcattttcg cagcttcact aataatttga tttgtcgcag
     4201 gtaaaataaa atctgcaaat tgaatttctg caattggtct tttacctacc atagctgcac
     4261 caatggcagt tccaacaata tttgactcag ctaatggcgt atcgataact ctgtcttcac
     4321 catatttttg ttgcagtcct tgagtagtac caaatacgcc accttttcta ccaacatctt
     4381 caccaagaat aaacacatct ttattttgtt gtaatgctaa gtcttgtgcc tggcgtatcg
     4441 cctctaaata agataattta gccattagtt aagactccct tcttcgtaca caaatgcata
     4501 ggcttcttcg acacttggat atggcgcgtc ttcagcagcc tttgtcgctt tattgatgat
     4561 gtctttatgc tccgcttcta tttctgccaa ccaagcatca tcgataatgc cagctgaaag
     4621 caactctttt ttgaactttt cattgcagtc tgctttttta agcgtttcac gctcttcttt
     4681 cgtacgatat tggtcgtcat catctgatga atgagctgtc atacgacttg ttactgcttc
     4741 aatcaaagtt gaaccttgac cagaaatagc tcgatctctt gcttctttca tcgctttata
     4801 cattgctaat ggatcattac catctacttg ttcaccatgt ataccgtaac caagtgctct
     4861 atccgataat ttttcagctg cgtattgtaa tgaatcaggt actgaaattg catatttatt
     4921 atttataatg acacatacaa aaggaagttt gtgtacaccc gcgaagttta aaccttcatg
     4981 gaagtcacct tggtttgagc taccttcacc aacagttgct gttgcaattt tcttcttacc
     5041 atccattttc aaagctaaag cagcaccaac agcatggggt atttgagttg ctaccggtga
     5101 actttgagac aaaatattct tagctctact actaaagtgt gatggcattt gttttccacc
     5161 agagttaaca tcgtctttct ttccaaacgc tgataaaaac gtatcatacg ctgagatacc
     5221 catataagta acgaaagcta gatctctata ataaggcgct gtaatatcac cttcttctaa
     5281 tgcgtatgcc atcccaatct gagttgcttc ttgtccttga ccacttacaa caaatggaat
     5341 tttacctgca cggttcaata accacagtct ttcatctatt tttctaccta aatccatcca
     5401 tttatatatt acttttaggt cttcttcgct aaggcctaat gatttataat caatcatgtt
     5461 aaatcctcct atttatacgt gaatagctct actttctgct ttcaatccta attccatcaa
     5521 cacttcagag atggaaggat gtgcgtgtgt tgttagtcct aattctaatg ccgagccatt
     5581 catgaactgt aacagtgatg cctcattaat caattctgtt acatgtggac caatcatatt
     5641 aatacccaca atttcttcag ttgattgatc aatcaccatt tcgctatacc cttcgtttgt
     5701 gtcatggcta tcaatcactg ctttaccaat tgctttaaat ggtactttaa aacttttaac
     5761 tttcattccc tctgcctttg cttgttcaat gtttaaaccg atagaagcaa tttcaggttg
     5821 tgaataaata cacttaggca tcatgttata gtttactggg attgggttcc cctcaaacat
     5881 atgatcaaca gccacaacac cttcttttga tccaacatgt gccaattgta attttcctat
     5941 acaatcacca gctgcataaa tatgtttatc ttcagtttgt tgaaattcgt tcgttaaaat
     6001 atgtcctgat gttgaaagtt ttattttagt gttgtttaaa ccaatatctg atgtgttagg
     6061 ttttctacca atcgatagca acactttatc tactttaatt atgtctgagg aaatttcaaa
     6121 cgtaacacca tcttcgttaa catttatatc attttcagaa agttttattc cctcatagaa
     6181 tttaacacca cgtgctgaca atgatttttt taatagttgt gaagcttgtt tactttcagt
     6241 tggtaaaatt ctttcacctg cttctataac tgttacgtca acacctaaat ctatcatcaa
     6301 tgatgcaaat tccattccga taacaccacc accaataata ccaatacttg atggtaacgt
     6361 ctttaatgat aatatatcat cgctagataa aattttatca tgatcaaatg ataagaatgg
     6421 caactctgca ggcgaagaac cagttgcaat taatacaaat tggttgggta ataagtctga
     6481 ttcaccatct tcatattcga cagaaattgt gccactttga ggtgaaaata tagatgtacc
     6541 tagaatacgt cccgtgccat tataaatgtc aatgtgattg tgttgcatta aatgctttac
     6601 accttgatac atttgattaa taatgtcttc ttttcgtgcc aacatatttt caaaattaac
     6661 attagcatct ttgacatcaa cgccaaacat tgctgcctgt tttactgttt gaaatacttc
     6721 agcagattta agcagcgatt tagtaggaat acaaccttta tggagacaag tacctcctaa
     6781 tagttgtcgt tccactattg ccactttttt acctaattga gacgcacgta tcgcagcaac
     6841 atatcctgca gtacctccac cgagaacgac taaatcatat tgtttctctg acatgttctt
     6901 actcctaact aatgatatat atccattgaa aatttattaa tacatagttt tcatgtccat
     6961 taattaccta ttttacatga ttgtctattt agtttgaatg cacataaata aatccataaa
     7021 tgagtattca acacactttg gaaattatct acgtctttga ttgtgttgta tcatttctct
     7081 tgcattttct cgagttaaat cagtaacact tgcacctgaa atcattcgtg caatttcatc
     7141 tactttatca tcgctaatta actcttgaac ttgtgttgtt gtacgatcat cttttgatga
     7201 tttcgaaatt aataaatgat ggtcgctcat cgatgcaact tgtggtaagt gagagataca
     7261 aataacttgt atatattctg ctatatctcg cattttctct gccatttttt gtgcagcttg
     7321 tccagataca cctgagtcaa cctcatcgaa taaaattgca gtttgacctc tcgatttaac
     7381 aaaaatactt tttaacgcta acataattct agaaagttct ccacctgacg caattttatt
     7441 taaacttttt aatggttccc ctttatttgg actgattaaa aattctacaa attcgattcc
     7501 atcaatattc ggttcttcta attttttaaa tgaaatctca agatttgcgt ctttcatttg
     7561 taagttttga atttccgata caatgtggtc tcttaactcc ctagcgacta tacgacgctg
     7621 ctttgataac gcttgtccaa cctctataac ttgattatac aatgcattta tttcttctcg
     7681 taactgcgat gtactttgtt catagttttc aattttattg atttcattat taagcttttc
     7741 ttgatatgcg attaattctg aaatatcttt tccatattta cgttttaaat tattcagcaa
     7801 attcatacga gattcatact cgtttaacac ttgttcgtcg aattctgtat tagccatttc
     7861 atcatataac tcatgttttg catcttctaa aatgtagtaa aattgatcaa tatcttcttt
     7921 taatttgtca tatttgtttg gaactatatc gtttattgtt aacaaatggt tgcttagttc
     7981 atataaacga tcagtgatag cattttcatc cgttaatgtc atatgtgcgt tattaagcgc
     8041 taagcttaat ttttcagagt tttgaatgcg tttaatatct atttcaagtt gctctatttc
     8101 gccttctttt agatgtgctt cagacaattc ttctaattgg aatttcatta aatctaaacg
     8161 ctgtagcaat gcttggtctg ctgattctaa atcttctaac tcttgctttt tggctttata
     8221 attttgaaaa gtttggtgat atttatccaa caaatcttga taacgtgatt ctgcgtaatt
     8281 atccaataat gttaaatggt atttttgttt caacaaagac tgcgtttcat gttggccatg
     8341 aatatctaat aattcttgca taacttttcg taaatcttgt aaagtaactg tttgattatt
     8401 aattttacaa agacttttac cagagctgaa aatttcccgt ttaactaata aaaaatcttc
     8461 atctacatca atatccatat ttttcaatat atgtatagca tctttactct cgtcaatatc
     8521 aaatatacct tcgatgacag cctttttttc accatgtctt acaaaatcag atgaagctct
     8581 cattccaatt aattgtccaa ttgcatctat aataattgat ttacctgaac ccgtttcacc
     8641 acttaaaaca gttaaaccat cagaaaattg aatttctaat tcttcaataa tagcaaattg
     8701 cttgattgat aaggtttgta acataaactc atcgcatcct tataacaaat tgaaaattct
     8761 tgacttgatt tcatcacttg cctctttgct tcgacaaata attaaacaag tatcatcacc
     8821 acaaattgtg cctagtactt cttcccaatt gatttggtct aatatagctc caatagattg
     8881 tgcattacca ggtaatgttt ttagaacaag taaattatca gtaccatcta tattaacaaa
     8941 ggaatccatt aaataacgtc ccaatttttc taaaggatgg aattttctat catttggtaa
     9001 actataaaca tattgacctg aaggtatagg tactttaata agttgtagtt ctttaatatc
     9061 acgagaaaca gttgcttgag tgacatttaa atcataatcg tttaatcgtt taactaattc
     9121 atcttgtgtc tctatctgtt catttgaaat aatttctcta atttttatat gcctaaccga
     9181 ttttttgggc acagtaaaca cctctgatta cgaatattta tacatttatt ttaacacatg
     9241 cactgattta cgactactaa acacctttac gtaaaaaggg taaacatggt ttatctatct
     9301 tggttatcta tttataaata tttttcatat tacgcataac aattgcttaa aatatgtata
     9361 aaaatgaata tatgtgtaat aaacttgcta attattagat ttaataagcg tcaattgttt
     9421 gaacatattt aattaaaatc acattgatat cacagatacg aatattgtcg tatagaaatt
     9481 gaaaattcta ttttttaaat gaaagtcttc aacataattt taagtttcaa catgagaaaa
     9541 atcgattaac aaacaacgtc agttgaatat gccttttgag acatttcaaa ctttacaatt
     9601 gttgctaatc gatatatttg cttttagtga tccctgctat aaaataaatc aacgatttct
     9661 aataagtgtt ttgtattgaa ttgttcatca atttgcgtta gttcatccac tgctgcgtct
     9721 ctatgataag tcaatttatc ttctgcgcca tctttcccta ataaactcac gtacgtactt
     9781 ttattatttt caagatcgct gcccactttt ttacctaact ttgcttcatc accatagcag
     9841 tctaataaat catctttaat ctggaacatc atacctaaat gataactata actttctaaa
     9901 tgttctttag ttgtatcatc gacattagcg atatctgctg cactcataac cgcaaaagtt
     9961 aataatgctc ctgtttttgt tttgtgtatc atttccaaag tttcaagatc aattggttgg
    10021 ccttcgcttt gcatatctaa catttgaccg ccgaccattc caacatgacc acttgctatt
    10081 gacagccgtt gtagaacttt tatttttact tcatcagtta atctatcatc acttgaaata
    10141 agttcaaatg ctttagttaa taaagcatca cctgctaata tcgcagtcca ctcaccatat
    10201 actttatgat ttgttaattt tcctcgtcga taatcatcat tatccatcgc tggtaggtca
    10261 tcatgaataa gtgaatatgt atgaatcatt tctagtgcaa ttgcgctctt catacctaac
    10321 tcatactcgg tatttagtga atctaaagtg agtaataaca gaactggtcg gatgcgttta
    10381 cctccagcat ttaatgaata caacatactt tcttctagct gagtatccat tactgattta
    10441 tttatcgcaa ccgataattc attattgact tcatctatta atttattcat cggtagattc
    10501 gtcatttttt acatcctcag cttcttcttt tattaagtca ttcacctttt tttcggcatt
    10561 ttttaaagtt gtgtcacaag ctgctgatag tttcatacca cgttgatata aatctaatga
    10621 ttcctctaaa gatactgttt cattatctaa tttttgaaca atttgctcta attcttgcat
    10681 catttcttca aaactttgcg tttctttagt cattattaca ccttactttc gtaacttttg
    10741 catctactaa gccatctttc attgttaatg tcaattgatc attttctgtt aaatctttag
    10801 tactcgtaat gacttcgtct tttttattaa caattgcata tccacgcaac attgtattag
    10861 ttggacttaa attgtttaag ttttctactt tatttttcaa atcattttta taacttaata
    10921 tcttagaatt caataattta acaagttggt ttgtcaattg aagattattt tgttgttctt
    10981 gattaacact acttagtaat gcttttaaat tataacgttg ttgcaacagc attaatcgat
    11041 gtctctgttg ttcaaaagtt gcctgaattt gttgtttcag tctcttttct aaatcatctc
    11101 gacgttgtat ctgttgatca tacaataaag ttggttgttt aaacttgtaa tacgatgaca
    11161 aatgttcaac atgtttacgt tgttgttcta aatgtttctt gatgaaacga gtcaatgtaa
    11221 actgatattg ctgtatttgt tgcagcaatt catattggtc tggtgttgca ataacagcag
    11281 cttgagttgg agtcgcagct ctgatgtctg cagcaaaatc acttaatgta aagtctgttt
    11341 catgaccaac tgctgatata atcggtgtct tacaattata tattgcacgg acgacagctt
    11401 cttcgttgaa attccataaa tcttctatgg atccaccgcc tcgacctaca ataatggtat
    11461 ctacacctaa actatctgca tattcaattt tttcaataat gtcgtctttt gctttttcac
    11521 cttgaaccaa agtactaatt tgtatttgtt cagctaatgg aaaacgacta tttatcgttg
    11581 aatggatatc tcgaattgcg gcacctgtac tcgctgttaa aactgcaatt tttttaggaa
    11641 acttaggtat tgatttctta ttcgctttat caaaacaacc ttcttcagtt aattttttct
    11701 ttaatgcttc taatttttga tataagttcc ctataccatc taattgcatt ttatttacat
    11761 aaatttgata gtttccacga cgttcaaaaa cagaaacacg tgcttctaat aagacttcat
    11821 ctccttcttt aggttcgaag tttaatttag aagcactacc tttgaacatc atggcactta
    11881 taacgctttc tttatctttc acattaaagt ataaatgacc acttgaatgc tttttgaaat
    11941 ttgaaagctc acctttaatc aatacagatt ggagatgtgg atcttgatca aatttatatt
    12001 taatatattt cgttaaagct gaaacactta aataatctga catataacat cactcaattt
    12061 tattttttta tattactcaa tacaccattt ataaatttat aatgatcatc atcactgaat
    12121 tgttttgtta attcaactgc ttcattcatt acgactttag caggtgtatc actgtgtaat
    12181 atttcatatg ttgccattct taaaataata cgatccgttt ttaataaacg tgcaatagtc
    12241 caatctttta aataaggact aattgtctcg tctaatacag gttcgtgacc tttaacgcca
    12301 gaaactagcc aatgaataaa ttcgaagtct aaatctggat tatcgtcttt aataaagctt
    12361 atcgcttcat ttatcgttaa atcactgtcc ttcatttcta attgaaataa agtttgaaaa
    12421 gcttgcactc gggattcttt acgactcatt tttaactcct tcaaacgttt gtatttttct
    12481 ttatttaatt actgaattag gtatgacatt acttttcaat aacgatttgt gtaatgtgaa
    12541 tattaatttg cttaggttct atcgctgtca tattagaaat tgaattaaaa attgacgttt
    12601 gaattttgtt tgcagttttt gaaatattaa caccatgttt taatgcacaa tatacatcta
    12661 tatatatgcc atcttcttta ctctcgattt ttaaatcacg gcttaaattt ttacgactaa
    12721 ctttttctaa atttgtttct tttaattcag caaaatggcc agtgatgcct tcgacttccg
    12781 aagtagctat acttgcaata acagatagca cttctggcgc tatttctact ttacctaatt
    12841 ttgaatttga ataatcagtt actttgacca tggattgacc tcctattaac cttcatcatt
    12901 cataatgcta ttttgctcta aaaagtttgt attaaattta ccgcttctaa atatatcgtt
    12961 attcaataat ttaatatgga atggaatagt tgtatcaata ccaagaacca caaattcact
    13021 tagtgcacga atgccagcca taatcgcttc atctcgtgtc ggttcatgta tgattaattt
    13081 cgctaccatc gaatcataat atggcggtat cgtataatta gtataacatg ctgactctat
    13141 tcgaacacca tatccacctg gtgcaagata ttgctcaatt ttacctggtg atggcataaa
    13201 gttcttgtaa ggattttcag cattaattct aaattcaatt gcgtgtcctg ttaatttaat
    13261 atcttcttgt ttatacggta acacgtcacc catagcaact tgtaattgta atttaactaa
    13321 atcaattcct gttaccattt cagttacagg atgttctact tgaatacgtg tattcatttc
    13381 cataaaataa aatttattat catttaaatc atatataaac tcaattgttc ccgcattttc
    13441 ataatttaca gctttcgctg cacgaactgc ggcatttccc atttcacgac gtgtttcatc
    13501 atctaaaatt ggggaaggtg cttcttccac taatttctgc atacgtcttt gaattgtaca
    13561 atcacgttct cctaaatgaa ttacattacc atagctgtcc ccaacaattt ggatttcaat
    13621 atggcggaag ttttcgatga atttctccat ataaagtcca ccattaccaa atgcagtttg
    13681 agcttcttgt tctgtcattc ggaagccagt ttcaagttct ttttcatcac gagcaacacg
    13741 gatacctttt ccgccaccgc cagcagtagc tttaatgatg accggatagc caattttttt
    13801 ggcgattttc ttagcttctg agacgtcttt cattaaaccg tcactaccag gaacaactgg
    13861 aacattggct ttgatcattt ctgccttagc aacatctttg atacccattt tttggataga
    13921 ttgataactt ggtccaatga acttcaattg gcatgcttcg cataattctg caaaatcagc
    13981 attttcagct aaaaagccat aacccggatg aacgccatca caacctgtag aagttgcaat
    14041 agataagatg ttcggaatat ttaaatatga atctttagac aaagtgggac ctacgcaata
    14101 tgcttcatca gcaatttgag tatgtagcgc atctttatcc ccttcagaat agattgcaac
    14161 agtttggatg cctaaatcac gacaagcgcg aataatccta actgcgattt caccgcggtt
    14221 tgcaattaaa acctttttca ttatttcacc ttaaataacg gttggccata ctctaccatt
    14281 tgtccgtctt ctactaagat ttcaacaatt tcacctgaaa tttctgcttg aatttcatta
    14341 aatagtttca ttgcctctaa aatacacact gttgtttcat ttgaaacagt gtccccaact
    14401 tgcacatatg cttcttcgtc tggagatggc gatttgtaaa atgtacctac cataggtgca
    14461 ttaattgttt tgtgattatc tgaagttggc tttggagctt cagttttatt gctatcagtt
    14521 gattgtgctt gaggcatagg cattgccgca gcttcaactg gcatttgtga gatttgtggc
    14581 gtgataatct cagtttcttt ttctttctta agcgtcactt tgcctttagt atcttcaata
    14641 ttgatttccg ttaaagttga tttatccaga atttcaatta attctttgat ttctttaaag
    14701 ttcattatta ctgactcctt cagtttgttt tcatctaccc gtctatttta cttgagacaa
    14761 ctcttcaatt caagcatgtt catattgctg gcgacattat aagtctatcc caaagttata
    14821 ataaaaccac attttaaatt aaaaacactt gtgtatttat tacttaacat tgactcatct
    14881 taactcttga ttaaaaattt tatgcattca gttaaacata tcaatgttat ttttattagc
    14941 tattatgttt tatcctcaac aatatgatat gtagatatat acctctactc atgtctatgt
    15001 tttttcacta ccaattagac gattcgtttt atattttaaa aattatttta aaaataaaaa
    15061 gcctcatatc agcatataac ctataatcat tttaggcaat agctttatga gacttattta
    15121 gaatatctta cgttaaatca gtatagtgaa tacaagctat ttgttaacaa attagagatt
    15181 atcctcttga aatgtagctt ccatcaccag tgttgataat taaaacgtca ccttcgttta
    15241 caaataaagg tacatttaat gtataaccag tttcaacagt tgccgattta gtggcaccag
    15301 ttgcagtatc acctttaata ccaggttctg tttcagttac tgttaattca acagttttag
    15361 gtaattcaac accgatagtt tcaccttcgt atgtttgaat ttgtacttcc ataccttctt
    15421 ttaagtaatt caattcttct tttaagtaat cacttgaaag ttctgtttgt tcaaagcttt
    15481 cattatccat aaatacatga ttatctccgt cagcatataa atattgcatg cgacgatttt
    15541 caatcattgc tggttcaact ttttcaccag ctctaaacgt tttctcttga attgcaccag
    15601 ttcttaaatt acgtaatttt gaacgaacga atgctgaacc tttaccaggc tttacatgtt
    15661 ggaagtctat aactttccaa atagcgttat caacagaaat tgttaaacct gttttaaaat
    15721 cattaaccga aatcattcag tttcctcctc attttacacg cttatgttaa aactataagg
    15781 tcttttgtgc atttagtaaa gacttgacaa ccattttctg taattaaaat atcatcttct
    15841 attcttatac cgcccaaacc ttctatataa acaccaggtt ctactgtaac acagttgtta
    15901 acttgaagtt tatcttgtat cgtacgagcc agcattggcc cttcatggat ttctaaacca
    15961 ataccatgtc ctagtgaatg tccaaattct tttccatacc cttttgactc taaatagttt
    16021 cttgaaatgg catcagcttc tgcaccagtc atgccaggtc taatctcatt aattgctttc
    16081 atttgagatt caagtactat ttgatatatt tctttcagtt taggatctgg ttctccaata
    16141 gcaaatgttc tagtaatatc tgaacaatag ccgttataat acgcgccaaa atctaatgta
    16201 atcatgtcgc ctttttcaat aattttatca cttgcaacac catgtggtaa tgcacctcta
    16261 tgaccagatg ctacaatcgt atcgaatgat ggtccatctg ctcctaattc tagcattttg
    16321 ctttctaata ttgcctttaa ttctttttca gtcatgcctg cttttacaac agttaaaata
    16381 tattcatatg tttcatcaac aatattagct gctttttgaa ttaaagcaat ttcgtcagca
    16441 tctttgacgt ctctaatttt atctacagta ttagaaatgc ttattaatga tatacggctt
    16501 ttatttaatt caaggtatgt atcataactt acatgatgcc cctcaaaacc tacattttca
    16561 aaattttctt ggtgtagcaa ttctttaatc tcaccaataa tagtagattt acgattaata
    16621 atttcataat ttggcgcctg cttagttgct tgatcaatat atctaaagtc tgttatcaaa
    16681 tattgtttat ctttagatat gataagtgct ccactggtac cagtaaaacc tgataaatat
    16741 cttctattgt aatccgaaag aatgataatc gcatctaaat gtttttgttc taaaatacga
    16801 tgcacttgtg ttattctgct catcttatta cctcctagtg aaacgtttgt gcatttcaac
    16861 tttatacatt aaaataatat cataataagg ataaaaaata atagatattg attttaggga
    16921 gatagtaatg aaaaaattgg tttcaattgt tggcgcaaca ttattgttag ctggatgtgg
    16981 atcacaaaat ttagcaccat tagaagaaaa aacaacagat ttaagagaag ataatcatca
    17041 actcaaacta gatattcaag aacttaatca acaaattagt gattctaaat ctaaaattaa
    17101 agggcttgaa aaggataaag aaaacagtaa aaaaactgca tctaataata cgaaaattaa
    17161 attgatgaat gttacatcaa catactacga caaagttgct aaagctttga aatcctataa
    17221 cgatattgag aaagatgtaa gtaaaaacaa aggcgataag aatgttcaat cgaaattaaa
    17281 tcaaatttct aatgatattc aaagtgctca cacttcatac aaagatgcta tcgatggttt
    17341 atcacttagt gatgatgata aaaaaacgtc taaaaatatc gataaattaa actctgattt
    17401 gaatcatgca tttgatgata ttaaaaatgg ctatcaaaat aaagataaaa aacaacttac
    17461 aaaaggacaa caagcgttgt caaaattaaa cttaaatgca aaatcatgat aggagtcttt
    17521 taatgcgtaa tataatattt tatcttgtac ttattattgc tgcgattgga ttagtaatga
    17581 atctagatgc ctttattttt tcaatcgtca gaatgttaat cagctttgct gtaatagctg
    17641 gtattattta tctgatttat tatttcttca tcttaactga agaccaacgc aaatatcgca
    17701 aagcaatgcg taagtataaa agaaatcaaa gaagaaaata gataaaaaaa cggaagcact
    17761 tgtaggtaaa atagtctacg tgcttccatt ttttattcta aaaactactt tctaaacatc
    17821 cattcatctg aacgatattt ttcagttaat tcttccactt ctgccaattg agcttctgtt
    17881 aattcaagtg gcttaaattc tatatttaaa cctttcttaa aacctttctc gaaagcttct
    17941 tccatttgac taatagtaat gtgttcatct gaaatatcat tgatggcaac tgctttttca
    18001 acgaatgcct ctttcatttt taattttaat ctttcatttt tataaataaa catatcaaac
    18061 agttcatcaa tatcaatatc ttgtaaaatc gaaccgtgtt ggaggattac gcccttttgt
    18121 ctcgtttgag cactcccagc aatcttacgg ccttcaacaa ctagctcata ccaacttggt
    18181 gcatcaaaac acactgaact tcgaggttgt tttaattttt gacgctcttc aggcgtttta
    18241 ggtaccgcaa aataagtatc aaatcctaag tttttaaatc cttctaataa tccttgtgaa
    18301 atcactctgt acgcttctgt aactgtagaa ggcatattcg gatgcgattc aggcacaatc
    18361 acactgtaag ttaactcttt atcatgtagc accccacggc caccagtttg acgccttacg
    18421 agaccaaaac ctttctcttt aaccttatca atatcaattt ctttttgtag cctttggaaa
    18481 taccctattg ataatgttgc aggattccat gtgtaaaaac gtataactgg atcaatttca
    18541 cctctagaga caaaatttaa taacgcttca tccattgcca tattataata tgggtcttta
    18601 cttcctgtat taataaaatt ccaagtttca gtcatattca agaactccta tcagcaaaat
    18661 gtataatatt tgattcgcta attaatcaat ttaactaaat gaataataat tgcaattctt
    18721 tagtgaaata ttttgataat ttgacctaac agtcttataa ttatattatc gtttaattag
    18781 ggaggatgca agatgagtgc tagtttgtac atcgcaataa ttttagttat agcaattatt
    18841 gcttatatga ttgttcaaca aattcttaac aagcgagctg ttaaagaatt agatcaaaat
    18901 gaattccata atgggattag aaaagctcaa gtcatcgatg ttagagagaa agttgactat
    18961 gactacggtc acattaatgg gtctcgcaat attcctatga caatgttcag gcaacgattc
    19021 caaggattaa gaaaagatca accggtatac ttatgtgatg ccaatgggat tgctagctat
    19081 agagccgctc gtattttgaa aaagaatgga tatacagata tctatatgtt aaaaggcggc
    19141 tataaaaaat ggactggaaa aataaagtct aaaaaatagt ttttgtaaat ttaatatacg
    19201 atttaataaa atctgagtgt taattgatca tcaataacaa tactcagatt ttaatttttt
    19261 aacaaagtct gttactatat ttctctagct tcactgatca ttaaacttag tttcagcata
    19321 ataaagaaag ttcagctcat tttcaatacg attcaattac cgcaatctaa aaaatgaaaa
    19381 gacaatttct atgaaagaat aataccaaac cctaagagtt attacttcgg tttagttttc
    19441 ttgtttaaat agaaattgtc tttttcaatt gattttgaaa ccattatcct taaatcttca
    19501 tacaaagtta gaataataat tctcggaata tgtgtttaat actttatttt tcctgtttaa
    19561 gattttcaaa ctttaatatt ggtttacgag cagctgtagc ttcgtctaat cgatcaatca
    19621 cagttgtatg tggtgcttct agcactttat caggatcatt tttagcttct tcagcaatac
    19681 taattaatgt atcgataaaa taatcaagtg tttctttaga ctctgtctca gtcggttcaa
    19741 tcatcatacc ttcttcaaca tttaatggga agtatattgt tggtggatgt acaccgaaat
    19801 ctaataatcg cttagccatg tctaaagtac gtacaccaaa ttctttttga cgcacaccac
    19861 ttaacacaaa ctcgtgttta caatattgtt tataaggtat ttcaaagtgt ttagataagc
    19921 gtgctttaat ataattcgca ttaagaaccg ctgcttcaga aacctcttta agtccagttg
    19981 ctcccatagt tcgaatatac gtataagctc ttaagtaaat accaaagtta ccataaaatg
    20041 gttttacacg tccgatagaa tttttaatgt cattatcata tttaaatttg tcgccatctt
    20101 taataaccat tggctttggt aagtaacttg ctagttcttt tactacaccg actggacctg
    20161 aaccaggacc gccaccacca tgtggaccag taaatgtttt atgcaagttt aaatgaacag
    20221 catcaaatcc catatctcct gggcgaactt tgtccataat agcgtttaaa ttcgcaccat
    20281 cataatataa tagaccacca gcattatgga cgatttcacg gatttccata atattttttt
    20341 cgaaaatacc taaagtgttt ggattagtta acataatagc tgctgtattt tcatttacaa
    20401 cacgtttcaa gtcatcaata tcaacttcgc cacgttcgtt tgattttaca gtaactgatt
    20461 taaatcctgc aaatgaagct gaggctggat tcgtaccatg cgcagaatct ggcacaatga
    20521 cttcatcacg atgaccttca ccattattct catggtaagc tttaaatatc atcaatgcag
    20581 tccattcacc atgtgcgcca gcagctggtt gtaatgtcac ctcatccata ccagtaattt
    20641 cttttaattc ttcttgcaaa ctataaataa tttctaatga accttgaact tgatcttcat
    20701 cttgtaatgg atgtgattca ctaaatcctg gtattctagc aaccttttca ttaattttag
    20761 ggttatactt catcgtacat gaacccaatg gataaaatcc gttgtctaca ccgaaatttt
    20821 tatttgaaag ttcagtataa tgacgtacta agtctagttc agcaacttca ggaaactccg
    20881 ctttgttttt acgaataaat ttatcatcta acaatgactc aacagaattt gttttaatat
    20941 cactttttgg taatgaatat gcatatctgc cttcacgaga tctttcaaaa attaatggac
    21001 ttgatttact agtcatttaa ctcaccagcc ttttctacaa atgtatcgat ttcatctttt
    21061 gttcttaatt cagttacagc tattaacatg tgatttttaa agtcgtctga aacaacacct
    21121 aaatcaaaac caccgataat attgtacttc actaattcct cgttaacttg ttgaattggt
    21181 ttgtcaaatt tgactacaaa ctcattgaaa gatgtaccat ctaatacttc aaaacctttt
    21241 ttaataaatt gttgtttagc atagttagca tgttctatat tttgaactgc aatatcatag
    21301 ataccttgtt taccaagtgc tgacattgca attgatgacg ctaaagcatt taatgcttgg
    21361 ttagaacaaa tattagatgt cgctttatcg cgtcgaatat gttgttcacg tgcttgtaat
    21421 gttaatacaa agccacgatt accttcatca tcttgtgttt gaccgactaa tctacctggc
    21481 actttacgca ttaacttttt cgtcgttgca aaatatccac aatgtggccc accgaattga
    21541 gcaggaattc cgaatggctg agtatcacct acaacaatat ctgcaccaaa tgaacctgga
    21601 ggtgtaagta atcccaatgc taatggattt gcatatacga taaataatgc ttttttatct
    21661 tcaataaagc tatgaatctt ttcaagatct tcaattgaac cgtaaaagtt tggatattgt
    21721 actgcaacag ctgctgtttc atcatccact gctgcttcta attttttcaa atctgtaaca
    21781 gtgccatcta aatcgatttc cactacttcg aattccttac gcgtcttagc ataagtatga
    21841 agtacttgta atgcttgata atgtaaacct tttgagacta caattttatt tttctttgtt
    21901 tgactaaatg ctaagataca tgcttcagca aagctagtca tcccatcata catagaagaa
    21961 tttgctacat ccatatctgt taattcacaa attaaagttt ggaactcaaa aatggcttgt
    22021 aattcacctt gagaaatttc cggttgatat ggcgtatatg ctgtgtaaaa ttctgatctt
    22081 gaaatcatag catccacaac tgatggcgcg taatgatcat aaacaccagc acccaaaaat
    22141 gatgtatgcg tttctttagt gatattcttg cttgcaatgc gatttaatct tctaagtaac
    22201 gttgtttccg cttcgccttc agcaatattt aaatctctat ttaataaaat gtcacttggt
    22261 acatcaccga ataattctcc tatagatttt gcaccaattg tttgtaacat ttcttgcttg
    22321 tctttttcag ttaaaggtat ataacgatga ctcacaatgc acaccccttt ttaattattt
    22381 atcaatttga tttttcttaa caattttcgc ttttaattga cgcttacgaa cttgaacaag
    22441 caactctcta cccatttcaa actcatctct ttttatcatt gcaagtgcaa ttgattttcc
    22501 tgatgatgga gactgtgttc ctgaagttac ttctccaata atatttccat ctaaatccat
    22561 aacttcataa ccagttcttg caattccttt ttcaagtaat tctaatccca ctgttcttct
    22621 tggtgcacca ttttcttttt gatcttttaa tacagattta ccaataaaat cagcatcaat
    22681 taatggttta cttgcaaaag cgataccacc ttcatatggt gtaattgatt cagttaaatc
    22741 ttgtccatgt aatggcaatc cagcctctaa tcttaatgta tcacgagcgc ctaacccaca
    22801 tggcataaca ttatactcta ataaaccatc ccaaattttt tcagtatcat cgatattaca
    22861 ataaatttca aacccatctt cacctgtgta acctgactga gataaaatga cgtttgctcc
    22921 aaataatttg acaccctgtt taaattcaaa cattttcatt tcagttacat cttcatcaac
    22981 taattgatta attaaatctc tagcttttgg tccttgtatt gctaattgac catattggtt
    23041 tgatacattt tgtacttcaa catcaaattt ctctttgtgt tttaaaatcc aattaa
//
LOCUS       DAKTIO010000020        22903 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.020, whole genome shotgun sequence.
ACCESSION   DAKTIO010000020 DAKTIO010000000
VERSION     DAKTIO010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22903
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.020"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            97..2139
                     /locus_tag="O0293_000631"
     CDS             97..2139
                     /locus_tag="O0293_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCY0279530.1"
                     /translation="MEIFETILIFIAVVILSSFVHTFIPKVPLAFIQIFLGMLLFITP
                     IPVQFNFDSELFMVTMIAPLLFVEGVNVSRVHLRKYIKPVMMMALGLVITTVIGVGLF
                     IHWIWPDLPIGAAFAIAAILCPTDAVAVQAITKGKVLPKGAMTILEGESLLNDAAGII
                     SFKIAVGVLVTGAFSLVDAVQLFLIASIGGAVVGLLIGMALVRFRLTLMRRGYENINM
                     FTIIQLLTPFVTYLIAELFHASGIIAAVVAGLVHGFERDRIMQVRTQLQMSYNHTWNI
                     LGYVLNGFVFSILGFLVPEVIIKIIKTEPHNLIFLIGITIVVALAVYLFRFVWVYVLY
                     PYFYLAISPFQKMMTKNDDDNPTTEKPPKRSLYALIMTLCGVHGTISLAIALTLPYFL
                     AGHHAFTYRNDLLFIASGMVIISLVVAQVLLPLLTKPAPKTVIGNMSFKVARIYILEQ
                     VIDYLNQKSTFETSFKYGNVIKEYHDKLAFLKTVEKDDENSKELERLQKIAFNVETKT
                     LESLVDEGQITNSVLENYMRYAERTQVYRQASLIRRMIVLLRGALLKRRVQTRVNSAS
                     SLSVTDNLMELNKINKLVHYNVVSRLSKETTKDNTLEVGMVCDGYLMRIENLTPSNFF
                     NSASEDTITKIKLNALREQRRILRELIDTDEVSEGTALKLREAINYDEMVIVDSMT"
     gene            complement(3299..4228)
                     /locus_tag="O0293_000632"
     CDS             complement(3299..4228)
                     /locus_tag="O0293_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCY0279531.1"
                     /translation="MKKLVPLLLALLLLVAACGTGGKQSSDKSNGKLKVVTTNSILYD
                     MAKNVGGDNVDIHSIVPVGQDPHEYEVKPKDIKKLTDADVILYNGLNLETGNGWFEKA
                     LEQAGKSLKDKKVIAVSKDVKPIYLNGEEGNKDKQDPHAWLSLDNGIKYVKTIQQTFI
                     DNDKKHKADYEKQGNKYIAQLEKLNNDSKDKFNDIPKEQRAMITSEGAFKYFSKQYGI
                     TPGYIWEINTEKQGTPEQMRQAIEFVKKHKLKHLLVETSVDKKAMESLSEETKKDIFG
                     EVYTDSIGKEGTKGDSYYKMMKSNIETVHGSMK"
     gene            complement(4225..5061)
                     /locus_tag="O0293_000633"
     CDS             complement(4225..5061)
                     /locus_tag="O0293_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCY0279532.1"
                     /translation="MLEFVEHLFTYQFLNRALITSIIVGIVCGTVGSLIVLRGLSLMG
                     DAMSHAVLPGVALSFLFGIPMFVGALITGMIASIFIGYITSSSKTKPDAAIGISFTAF
                     LASGIIIISLINTTTDLYHILFGNLLAITNSAFLTTIVIGSIVLILIIIFYRPLMIST
                     FDPTFSRMSGLNTTLLHYFVMLLLSLVTVASIQTVGIILVVALLITPASTAFLISKKL
                     YSMMIIASLISVISSIVGLYYSYIYNIPSGATIVLCTFVIYIITLFFTKFTNRKKRGS
                     LT"
     gene            complement(5055..5798)
                     /locus_tag="O0293_000634"
     CDS             complement(5055..5798)
                     /locus_tag="O0293_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCY0279533.1"
                     /translation="MLETKDLNLFLGNKHVLKNISLSIPVRGEIIGIMGPNGAGKSSL
                     IKSLIGEFNATGTKLLYNKPIQQQLQHITYIPQKAHIDLDFPISVEQVILSGCYKEIG
                     WFRRPNKSARDKLKQLLSDLELESLRHRQISELSGGQLQRVLVARALMSESEVYFLDE
                     PFVGIDFSSEKLIMTKIENLKQQGKLILIIHHDLSKAKQYFDRIILLNQTLRYFGDSE
                     EAMSVTRLNETFMSSTDCSDPSQRSNITC"
     gene            5920..6564
                     /locus_tag="O0293_000635"
     CDS             5920..6564
                     /locus_tag="O0293_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HCY0279534.1"
                     /translation="MLTEEKEDYLKAILTNNGDKNFVTNKILSQFLNIKPPSVSEMVG
                     RLEKAGYVETKPYKGVRLTEDGLTHTLDIIKRHRLLELFLIEILKYNWEEVHQEAEIL
                     EHRISDLFVERLDSLLNFPETCPHGGVIPRNNEYKEKYITTILNYEPGDIVTIKRVRD
                     KTDLLIYLSSKDISIGNEVEIVSKDEMNKVIIIKRNDNVIIVSYENAMNMFAEK"
     gene            complement(6644..7396)
                     /locus_tag="O0293_000636"
     CDS             complement(6644..7396)
                     /locus_tag="O0293_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HCY0279535.1"
                     /translation="MNYLSNFFNTTIQLNIYIIVIVAACYIAIHQYRHKPVLNYLDVI
                     LNYIPVLTHEFGHVLFNKLAGGRAKDLVIVTSPRERQQTLQQGFAITQSRHLAGQWLT
                     TIGGYFMPPIMLLIGLASSHYQIPSFFIFTYLLIFIYFLILTSRKGSPIVVITLISIM
                     LYFILKDENIVEIQLLVTMSYQYILGVLLGEVLQSSWTIAKLTFQRPSPQWDGSALKE
                     LSHVPIFIYSTIWIIFNLYAVNILLKYTHLIN"
     gene            7632..8396
                     /locus_tag="O0293_000637"
     CDS             7632..8396
                     /locus_tag="O0293_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HCY0279536.1"
                     /translation="MTVEERSNTAKVDILGVDFDNTTMLQMVENIKTFFANQSTNNLF
                     IVTANPEIVNYATTHQAYLELINQASYIVADGTGVVKASHRLKQPLAHRIPGIELMDE
                     CLKIAHVNHQKVFLLGATNEVVEAAQYALQQRYPNISFAHHHGYIDLEDETVVKRIKL
                     FKPDYIFVGMGFPKQEEWIMTHENQFESTVMMGVGGSLEVFAGAKKRAPYIFRKLNIE
                     WIYRALIDWKRIGRLKSIPIFMYKIAKAKRKIKKAK"
     gene            complement(8457..9251)
                     /gene="tagH"
                     /locus_tag="O0293_000638"
     CDS             complement(8457..9251)
                     /gene="tagH"
                     /locus_tag="O0293_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter
                     ATP-binding subunit TagH"
                     /protein_id="HCY0279537.1"
                     /translation="MNVSVNIKNVTKEYRIYRTNKERMKDALIPKHKNKTFFALDDIS
                     LKAYEGDVIGLVGINGSGKSTLSNIIGGSLSPTVGKVDRNGEVSVIAISAGLSGQLTG
                     IENIEFKMLCMGFKRKEIKAMTPKIIEFSELGEFIYQPVKKYSSGMRAKLGFSINITV
                     NPDILVIDEALSVGDQTFAQKCLDKIYEFKEQNKTIFFVSHNLGQVRQFCTKIAWIEG
                     GKLKDYGELDDVLPKYEAFLNDFKKKSKAEQKEFRNKLDESRFVIK"
     gene            9598..10410
                     /gene="tagG"
                     /locus_tag="O0293_000639"
     CDS             9598..10410
                     /gene="tagG"
                     /locus_tag="O0293_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter permease
                     subunit TagG"
                     /protein_id="HCY0279538.1"
                     /translation="MSAIGTVFKEHVKNFYLIQRLAQFQVKIINHSNYLGVAWELINP
                     VMQIMVYWMVFGLGIRSNAPIHGVPFVYWLLVGISMWFFINQGILEGTKAITQKFNQV
                     SKMNFPLSIIPTYIVTSRFYGHLGLLLLVIIACMFTGIYPSIHIIQLLIYVPFCFFLT
                     ASVTLLTSTLGVLVRDTQMLMQAILRILFYFSPILWLPKNHGISGLIHEMMKYNPVYF
                     IAESYRAAILYHEWYFMDHWKLMLYNFGIVAIFFAIGAYLHMKYRDQFADFL"
     gene            10509..11612
                     /locus_tag="O0293_000640"
     CDS             10509..11612
                     /locus_tag="O0293_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCY0279539.1"
                     /translation="MNVLIKKFYHLVVRILSKMITPQVIDKPHIVFMMTFPEDIKPII
                     KALNNSSYQKTVLTTPKQAPYLSELSDDVDVIEMTNRTLVKQIKALKSAQMIIIDNYY
                     LLLGGYNKTSNQHIVQTWHASGALKNFGLTDHQVDVSDKAMVQQYRKVYQATDFYLVG
                     CEQMSQCFKQSLGATEEQMLYFGLPRINKYYTADRATVKAELKDKYGITNKLVLYVPT
                     YREDKADNRAIDKAYFEKCLPGYTLINKLHPSIEDSDIDDVSSIDTSTLMLMSDIIIS
                     DYSSLPIEASLLDIPTIFYVYDEGTYDQVRGLNQFYKAIPDSYKVYTEEDLIMTIQEK
                     EHLLSPLFKDWHKYNTDKSLHQLTEYIDKMVTK"
     gene            11609..12670
                     /locus_tag="O0293_000641"
     CDS             11609..12670
                     /locus_tag="O0293_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCY0279540.1"
                     /translation="MRFTIIIPTCNNEATIRQLLISIESKEHYRILCIDGGSTDQTIP
                     MIERLQRELKHISLIQLQNASIATCINKGLMDIKMTDPHDSDAFMVIKPTSIVLPGKL
                     DRLTAAFKNNDNIDMVIGQRAYNYHGEWKLKSADEFIKDNRIVTLTEQPDLLSMMSFD
                     GKLFSAKFAELQCDETLANTYNHAILVKAMQKATDIHLVSQMIVGDNDIDTHATSNEE
                     DFNRYITEIMKIRQRVMEMLLLPEQRLLYSDMVDRILFNNSLKYYMNEHPAVTHTTIQ
                     LVKDYIMSMQHSDYVSQNMFDIINTVEFIGENWDREIYELWRQTLIQVGINRPTYKKF
                     LIQLKGRKFAHRTKSMLKR"
     gene            12733..13131
                     /gene="tagD"
                     /locus_tag="O0293_000642"
     CDS             12733..13131
                     /gene="tagD"
                     /locus_tag="O0293_000642"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HCY0279541.1"
                     /translation="MKRVITYGTYDLLHYGHIELLRRAREMGDYLIVALSTDEFNQIK
                     HKKSYYDYEQRKMMLESIRYVDLVIPEKGWGQKEDDVEKFDVDVFVMGHDWEGEFDFL
                     KDKCEVIYLKRTEGISTTKIKQELYGKDAK"
     gene            complement(13248..14543)
                     /gene="pbp4"
                     /locus_tag="O0293_000643"
     CDS             complement(13248..14543)
                     /gene="pbp4"
                     /locus_tag="O0293_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP4"
                     /protein_id="HCY0279542.1"
                     /translation="MKNLISIIIILCLTLSIMTPYAQATNSDVTPVQAANQYGYAGLS
                     AAYEPTSAVNVSQTGQLLYQYNIDTKWNPASMTKLMTMYLTLEAVNKGQLSLDDTVTM
                     TNKEYIMSTLPELSNTKLYPGQVWTIADLLQITVSNSSNAAALILAKKVSKNTSDFVD
                     LMNNKAKAIGMKNTHFVNPTGAENSRLRTFAPTKYKDQERTVTTARDYAILDLHVIKE
                     TPKILDFTKQLAPTTHAVTYYTFNFSLEGAKMSLPGTDGLKTGSSDTANYNHTITTKR
                     GKFRINQVIMGAGDYKNLGGEKQRNMMGNALMERSFDQYKYVKILSKGEQRINGKKYY
                     VENDLYDVLPSDFSKKDYKLVVEDGKVHADYPREFINKDYGPPTVEVHQPIIQKANTV
                     AKSMWEEHPLFTIIGGTCLVAGLALIVHMIINRLFRKRK"
     gene            14964..16691
                     /locus_tag="O0293_000644"
     CDS             14964..16691
                     /locus_tag="O0293_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0279543.1"
                     /translation="MKRENPLFFLFKKLSWPVGLIVAAITISSLGSLSGLLVPLFTGR
                     IVDKFSVSHINWNLIALFGGIFVINALLSGLGLYLLSKIGEKIIYAIRSVLWEHIIQL
                     KMPFFDKNESGQLMSRLTDDTKVINEFISQKLPNLLPSIVTLVGSLIMLFILDWKMTL
                     LTFITIPIFVLIMIPLGRIMQKISTSTQSEIANFSGLLGRVLTEMRLVKISNTERLEL
                     DNAHKNLNEIYKLGLKQAKIAAVVQPISGIVMLLTIAIILGFGALEIATGAITAGTLI
                     AMIFYVIQLSMPLINLSTLVTDYKKAVGASSRIYEIMQEPIEPTEALEDSENVLIDDG
                     VLSFEHVDFKYDVKKILDDVSFQIPQGQVSAFVGPSGSGKSTIFNLIERMYEIESGDI
                     KYGLESVYDIPLSKWRRKIGYVMQSNSMMSGTIRDNILYGINRHVSDEELINYAKLAN
                     CHDFIMQFDEGYDTLVGERGLKLSGGQRQRIDIARSFVKNPDILLLDEATANLDSESE
                     LKIQEALETLMEGRTTIVIAHRLSTIKKAGQIIFLDKGQVTGKGTHSELMASHAKYKN
                     FVVSQKLTD"
     gene            17050..18279
                     /locus_tag="O0293_000645"
     CDS             17050..18279
                     /locus_tag="O0293_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCY0279544.1"
                     /translation="MFLLINIIGLIVFLGIAVLFSRDRKNIQWQSIGILVVLNLFLAW
                     FFIYFDWGQKAVRGAANGIAWVVQSAHAGTGFAFASLTNVKMMDMAVAALFPILLIVP
                     LFDILMYFNILPKIIGGIGWLLAKVTRQPKFESFFGIEMMFLGNTEALAVSSEQLKRM
                     NEMRVLTIAMMSMSSVSGAIVGAYVQMVPGELVLTAIPLNIVNAIIVSCLLNPVSVEE
                     KEDIIYSLKNNEVERQPFFSFLGDSVLAAGKLVLIIIAFVISFVALADLFDRFINLIT
                     GLIAGWIGIKGSFGLNQILGVFMYPFALLLGLPYDEAWLVAQQMAKKIVTNEFVVMGE
                     ISKDIASYTPHHRAVITTFLISFANFSTIGMIIGTLKGIVDKKTSDFVSKYVPMMLLS
                     GILVSLLTAAFVGLFAW"
     gene            18534..18626
                     /locus_tag="O0293_000646"
     CDS             18534..18626
                     /locus_tag="O0293_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279545.1"
                     /translation="MFCYYKQHKGDNFSIEEVKNIIADNEMKVN"
     gene            18804..19637
                     /locus_tag="O0293_000647"
     CDS             18804..19637
                     /locus_tag="O0293_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCY0279546.1"
                     /translation="MNKTVKDLILVVLGSFIFAAGVNAFIISGNLGEGGVTGLAIILY
                     YAFHISPAITNFLVNAVLIAIGYKFLSKRSMYLTILVTILISIFLSLTESWQVETGNS
                     IVNAIFGGVSVGLGIGVIILAGGTTAGTTILARIATKYLDVSTPYALLFFDMIVVAIS
                     LTVIPLDKVLVTVISLYIGTKVMEYVIEGLNTKKAMTIISTNPDKLAKAIDEQIGRGL
                     TILNGHGYYTREEKDVLYVVISKTQVSKAKRLIKQIDKDAFLVIHDVRDVYGNGFLAD
                     E"
     gene            19919..20716
                     /locus_tag="O0293_000648"
     CDS             19919..20716
                     /locus_tag="O0293_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0279547.1"
                     /translation="MNRLHGQQVKIGYGDNTIINKLDVEIPDGKVTSIIGPNGCGKST
                     LLKALSRLLAVKEGEVFLDGENIHTQSTKEIAKKIAILPQSPEVADGLTVGELVSYGR
                     FPHQKGFGRLTAEDKKEIDWAMEVTGTDTFRHRSINDLSGGQRQRVWIAMALAQRTDI
                     IFLDEPTTYLDICHQLEILELVQKLNQEQGCTIVMVLHDINQAIRFSDHLIAMKEGDI
                     IATGSTEDVLTQEILEKVFNIDVVLSKDPKTGKPLLVTYDLCRRAYS"
     gene            20752..21756
                     /locus_tag="O0293_000649"
     CDS             20752..21756
                     /locus_tag="O0293_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCY0279548.1"
                     /translation="MTNRENPTPLKFLSYIIGLSMILLITLFISTLIGDAKIQASTII
                     EAIFNYNPSNQQQNIINEIRIPRNIAAVIVGMALAVSGAIIQGVTRNGLADPALIGLN
                     SGASFALALTYAVLPNTSFLILMFAGFLGAILGGAIVLMIGRSRRDGFNPMRIILAGA
                     AVSAMLTALSQGIALAFRLNQTVTFWTAGGVSGTTWSHLKWAIPLIGIALFIILTISK
                     QLTILNLGESLAKGLGQNVTMIRGICLIIAMILAGIAVAIAGQVAFVGLMVPHIARFL
                     IGTDYAKILPLTALLGGILVLVADVIARYLGEAPVGAIISFIGVPYFLYLVKKGGRSI
                     "
     gene            21753..22769
                     /locus_tag="O0293_000650"
     CDS             21753..22769
                     /locus_tag="O0293_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCY0279549.1"
                     /translation="MISSNNKRRQLIALAVFSILLFLGCTWSITSGEYNIPVERFFKT
                     LIGQGDAIDKLILLDFRLPRMMITILAGAALSISGAIVQSVTKNPIAEPGILGINAGG
                     GFAIALFIAIGKINADNFVYVLPLISILGGITTALIIFIFSFNKNEGVTPASMVLIGV
                     GLQTALYGGSITIMSKFDDKQSDFIAAWFAGNIWGDEWPFVIAFLPWVLIIIPYLLFK
                     SNTLNIIHTGDNIARGLGVRLSRERLILFFIAVMLSSAAVAVAGSISFIGLMGPHIAK
                     RIVGPRHQLFLPIAILVGACLLVIADTIGKIVLQPGGVPAGIVVAIIGAPYFLYLMYK
                     TKNV"
BASE COUNT         7745 a         3336 c         4054 g         7768 t
ORIGIN      
        1 tgtggtcgct aactcaattt atcctctaaa gtataattac aacgagatgt gatacttaat
       61 tttcaattaa acaatacttt ttagagaggt gaaagtttgg aaatatttga aacaattctt
      121 atatttatag ctgttgtgat actaagttcg tttgtccata ctttcatacc taaagtaccc
      181 ctagcattta tacaaatttt cttgggcatg ttactattta ttaccccaat ccctgttcaa
      241 tttaattttg attctgaatt gtttatggta acaatgattg cgcctttgtt atttgtagaa
      301 ggtgttaatg tttctagagt ccatttaagg aaatatatta agccagtgat gatgatggca
      361 ttaggattag tcattactac tgtgataggt gtaggtttat ttattcattg gatttggcca
      421 gatttaccta ttggagcagc atttgcaatt gctgccattc tttgtcctac tgatgcagta
      481 gcagtgcaag caatcactaa aggaaaggtc ttgccaaaag gagcaatgac aattcttgaa
      541 ggtgagtcat tattgaatga tgctgctggt attatttcat ttaaaatagc tgttggagta
      601 ttagttacag gtgctttttc acttgttgat gctgttcagt tgtttttaat tgcatcaatt
      661 ggtggcgcag tggttggttt acttataggt atggcattag taaggttccg attaacattg
      721 atgcgtcgag gatatgaaaa cattaatatg tttacaatta ttcaattgtt aacaccattt
      781 gttacgtatt taattgctga attgtttcac gcatcaggaa tcattgcagc agtagttgca
      841 ggacttgtac atggtttcga acgtgacaga attatgcaag tacgtacaca actgcaaatg
      901 agttacaatc atacatggaa tatactaggt tatgttttaa atggctttgt tttttcaata
      961 ttaggatttt tagtacctga agttattatt aaaattatca aaacagaacc gcacaattta
     1021 atctttttaa taggcatcac tattgttgtt gctttagctg tctatctatt tagatttgtt
     1081 tgggtttatg tcttatatcc ttatttttat ttagccatca gtccattcca aaaaatgatg
     1141 actaaaaatg atgatgataa tccaacgact gagaaaccac caaagcgaag tttatacgct
     1201 ttaattatga cgttatgtgg tgtgcatgga acaatttctt tagcaatcgc attaacgtta
     1261 ccgtattttt tagcagggca tcatgctttt acgtatagaa acgacttatt atttattgca
     1321 tctggtatgg ttattattag tttggtagtt gcgcaagtat tattgccatt attaacgaaa
     1381 cctgcaccta aaacagtaat tggcaatatg tcgtttaaag ttgctagaat ttatatatta
     1441 gaacaagtta ttgattatct aaatcaaaaa tctactttcg aaacaagttt taaatatggt
     1501 aacgtgatta aagaatatca tgataaatta gcatttttaa aaactgtaga gaaagatgat
     1561 gaaaactcta aagaattaga acgtctacaa aaaattgctt ttaatgtaga aacaaaaaca
     1621 ttagagtctt tagtagatga aggacaaata acgaatagtg tacttgaaaa ctatatgcgt
     1681 tatgctgaaa gaacacaggt atatagacaa gcatcattaa taagaagaat gattgtatta
     1741 ttacgaggtg ctttattaaa acgaagagta caaacgagag tgaactccgc atcttcactt
     1801 agtgttacgg ataacttaat ggaattaaat aaaattaata aattagtcca ttataatgtg
     1861 gttagtcgtt tgtctaagga aacaacaaaa gataatacac ttgaagttgg aatggtttgt
     1921 gacggttatt taatgcgaat tgaaaactta acaccatcaa atttcttcaa ctcagcaagt
     1981 gaagatacga ttactaaaat taaattaaat gcattgagag aacaacgtcg cattttacgt
     2041 gagttgattg atacagatga agtatcagaa ggtacagcgt taaaactaag agaagccatc
     2101 aattatgatg aaatggttat tgtagatagt atgacgtagt tcctaattat gctaaaaggg
     2161 attgatgaaa aactgaaggg cttttcatca atccctttta ttttagggga attgaataga
     2221 tagttttaaa ctatacgaat tattaatatt tgagatttaa ttgaaataag ttttaaaaat
     2281 tggaggagat agattaagcg aagtcattta aaggtgaagt taagtgtatt cacaaaaaat
     2341 agccacactc atatgacatc ggatgagtgt ggcttaagga tctatggggg gaggaaacca
     2401 tagatgttta ctttgatagg ccagattaaa tatcaaagta tgcgattatt tatagcttga
     2461 tgcaaaagtg gtatgcctat taaaagttac tgcacatagc ttttaatatt ccgttcaaag
     2521 gaaaggggca tacaattgaa caatctgtaa tagtactttt aaccagctat gctaaaagtc
     2581 tagtagggag aacagttgtc caatcacata agaacctcta acttcgttag tacgattaag
     2641 aaaagctttt tagttagtat gtaatacaat ttattgacgc gcgtgaatct cttttataag
     2701 agtgtgtagg gaatggcgtt gtataaattg tattagaaga acttctaacg catctctgtg
     2761 gttaaaagag atgaagggaa cgacagttta attaaaactg cataagaact tctagctttt
     2821 ctctctcgtt caaagagaag cagctgttcg cagtttaatc aaaaccacat aaagctttta
     2881 actttactct ttgatttaaa gagtgataaa tgtttacagt ttaattaaaa ctgcataaga
     2941 acttctagct tttctctttc gttcaaagag aagcagctgt tcgcagttta atcaaaacca
     3001 cataaagctt ttaactttac tctttgattt aaagagtgac aaatgtttac agtttaatta
     3061 aaactgcata agaacttcta gcttttctct ttcgttcaaa gagaagttct aataccacca
     3121 tatcgtgcga tcgggaacgg tatatatatt aataggaggg taatatatat ttaacgcacg
     3181 atatgggact attagccttc gactttgtta tgttgatgtg tggcctaaaa tattggagat
     3241 accaatattt taggttgcat caacatcaat tcatcttact tcattaaaac acagcgtgtt
     3301 atttcatgct tccgtgtaca gtttcaatat ttgatttcat cattttgtag taagagtcac
     3361 ctttagtgcc ttctttaccg attgaatctg tgtacacttc accaaagata tctttcttcg
     3421 tttcttcaga taaactttcc attgctttct tatcaacact tgtttctact aataagtgtt
     3481 ttaatttgtg ctttttaaca aactcaatag cttgtctcat ttgttcaggt gtaccttgtt
     3541 tttcagtgtt aatttcccaa atataacctg gtgtaatacc gtattgtttt gagaagtact
     3601 tgaaggcacc ttcacttgta atcatggcac gttgttcttt tggaatgtca ttaaatttgt
     3661 ctttactgtc attatttaat ttttccaatt gagcaatgta tttgttacct tgcttttcat
     3721 aatctgcttt atgttttttg tcgttatcga taaatgtttg ttgaattgtt tttacgtatt
     3781 taataccgtt atctaaactt aaccatgcgt gtggatcttg tttatctttg ttgccttctt
     3841 caccgtttaa atagataggt ttaacatctt ttgatactgc gataactttt ttatctttta
     3901 atgatttacc agcctgttct aaggcttttt caaaccaacc gttaccagtc tctaaattta
     3961 atccgttgta taaaataacg tcagcgtcag ttaacttttt aatatcttta ggtttaactt
     4021 catattcatg aggatcttga ccaacaggta caatactatg aatatcgacg ttgtctccac
     4081 caacattttt agccatatca tataaaattg aattcgtcgt tactactttt aatttgccat
     4141 ttgacttatc actgctttgt ttaccaccag taccacatgc agcaactaga agtaataagg
     4201 ctaataataa aggtactaat tttttcatgt taaacttcct cgtttctttc tattcgtaaa
     4261 ttttgtgaaa aataatgtga tgatataaat tacaaacgta caaagtacga ttgtcgcacc
     4321 actaggaatg ttgtaaatat agctgtaata aagtccgaca attgaactta tgacacttat
     4381 taaacttgct ataatcatca ttgagtatag ttttttacta attaaaaatg ctgtagatgc
     4441 aggtgtaatt aataatgcaa ctacaagaat aatacctacc gtttgaatac ttgctactgt
     4501 tactaatgag agtaacaaca tcacaaagta atgtaataac gtcgtattta gaccactcat
     4561 tctactaaac gttggatcga atgtagaaat cattaatgga cgatagaaaa taatgattag
     4621 aataaggacg attgaaccaa tcacaatagt tgttaaaaat gcactatttg tgattgccag
     4681 taaattacca aacagaatat ggtacaaatc tgtcgtagtg tttattaagc taataataat
     4741 aatccccgaa gctaagaaag cggtaaaact aattccaata gcggcgtcag gtttcgtttt
     4801 actactagat gtgatataac cgataaaaat acttgcgatc ataccagtta taagtgcgcc
     4861 tacaaacatt ggaataccaa ataagaatga tagggcaaca ccaggtaata ctgcgtgact
     4921 cattgcatct cccattaatg aaagaccacg taatacaatt aaactaccaa ctgtaccaca
     4981 aactatccct acaataattg aagttatcaa tgctcgattc aagaattgat atgtaaataa
     5041 atgttcgaca aactctaaca tgttatattg ctcctttgac tagggtcact acagtcagtg
     5101 ctactcataa atgtttcgtt taagcgagtg acactcatag cctcttcact atcaccaaag
     5161 tatcgtaatg tttgatttaa tagaataatg cgatcaaagt attgctttgc ttttgataga
     5221 tcatggtgga tgataagaat aagttttcct tgttgtttta agttctcgat ttttgtcatg
     5281 attaattttt cgctactaaa atcaattccg acaaacggct catctagaaa ataaacttca
     5341 ctttcggaca tcaatgctct tgctactagc acacgttgta attgtccacc acttaattct
     5401 gaaatttgtc gatgacgtaa agattctaat tctaaatcgc ttaataactg tttgagttta
     5461 tcccttgctg atttattagg tcgtctaaac catccaattt ctttgtagca acctgataaa
     5521 atcacttgtt ccacacttat aggaaaatct aaatcaatat gtgctttttg tggaatatat
     5581 gtaatatgtt gcagttgttg ttgtataggt ttgttatata acaatttagt accggtagca
     5641 ttaaattcac caattaaaga cttgataagg gaagatttac cagcaccatt cgggcccatg
     5701 ataccaatta tttcgccgcg tactggtatc gataaggaaa tgtttttaag tacatgctta
     5761 ttacctaaaa acagatttaa atcttttgtt tctaacaaac gtttatacct cctaattaaa
     5821 agtttaggct aacctaatta attgtataat aaactgagaa tatttatcat gtcaagtaaa
     5881 ttcgtgatat aatatagaca atgtatgtga ggtgaaagta tgttaactga agaaaaagag
     5941 gactatttaa aggcaatcct tacgaataat ggcgataaaa actttgtgac aaataaaatc
     6001 ttatctcaat ttttaaatat taagcctcca tctgtaagtg aaatggtagg acgtcttgaa
     6061 aaagcaggct atgttgaaac aaaaccatac aaaggtgtta gattaacaga ggatggttta
     6121 acgcatacgc ttgatatcat taagagacat cgactattag aattattttt aatagaaata
     6181 ttgaaatata attgggaaga agtacatcaa gaagcagaaa ttttagaaca tcgaatttca
     6241 gatttatttg ttgaaaggct ggatagcctg ttaaatttcc cagaaacttg cccgcacggc
     6301 ggtgtgattc ctagaaataa tgaatataaa gagaaatata taacaacgat tttgaattat
     6361 gaacctggtg atatcgttac aatcaaacgt gtgagagata agaccgattt gctaatatat
     6421 ttgtctagta aagatatttc tattggtaat gaagtggaaa ttgtatcgaa agatgaaatg
     6481 aataaagtaa ttatcattaa acgtaatgat aatgtaatta ttgtcagtta cgaaaatgca
     6541 atgaacatgt ttgctgaaaa ataaaataaa gaagccataa agatatccat gattgaactg
     6601 ataaagacat atggataatt gctttaggct tcttttttat tagttaattt atcaagtgag
     6661 tatatttgag taaaatattc actgcataaa gattgaagat aatccagatt gtactataaa
     6721 tgaagatagg tacatgactg agttctttaa gtgcactacc atcccactgt ggactcgggc
     6781 gctggaaagt caatttagca atcgtccaac tagattgtag aacttcgcct aataatacac
     6841 ctaaaatata ttgataactc attgtgacaa gtagttgaat ttctactata ttttcatctt
     6901 ttaatataaa atacaacatg atagaaatta aagttataac aacaatgggt gagccttttc
     6961 tagatgttaa aattaaaaaa taaataaata tcaataaata ggtaaatata aagaaactag
     7021 gtatctgata atggctcgac gctaaaccta tcaataacat aataggtggc ataaaataac
     7081 caccaatcgt tgtaagccat tggcctgcta gatgtctaga ttgtgtaatt gcgaatcctt
     7141 gttgtaatgt ctgttgtcgc tctcgtggac ttgttacaat gactaaatct tttgcacggc
     7201 caccagcgag tttattaaac agtacatgac caaattcatg tgttaaaaca gggatatagt
     7261 ttaaaatgac atctaaatag ttcaaaacag gcttatgtct atattgatga atagcaatat
     7321 aacaagctgc aacaataacg ataatgtata tattaagttg aattgtcgta ttaaaaaagt
     7381 ttgataaata attcattgtt aacctcatat aagatattaa tttaaagttt gcttatcact
     7441 tattataaat gatattggca tcaatagcgt tagactttag acttacctta gttaaactaa
     7501 ttttaatttt tgaaaaggtg aatatgtgtt aaaataaagc aaaatcattt cgatataaat
     7561 aggatgaata taaatactgt taatattgat tacactaaca taataatgaa ataagatagg
     7621 agattcctgt tatgactgtt gaagaaagat ccaatacagc caaagttgac attttagggg
     7681 tcgattttga taatacaaca atgttgcaaa tggttgaaaa tattaaaacc ttttttgcaa
     7741 atcaatcaac gaataatctt tttatagtaa cagccaaccc tgaaatagtg aattacgcga
     7801 cgacacatca agcgtattta gagttaataa atcaagcgag ctatattgtt gctgatggga
     7861 caggagtagt caaagcttcg catcgtttaa agcaacctct agcgcatcgt atacctggta
     7921 ttgagttgat ggatgaatgt ttgaaaattg ctcatgtaaa tcatcaaaaa gtatttttgc
     7981 taggggcaac taatgaagtt gtagaagcgg cacaatatgc attgcaacaa agatatccaa
     8041 acatatcgtt tgcacatcat cacggttata ttgatttaga agatgagaca gtagtgaaac
     8101 gaattaaact gtttaaacct gattacatat ttgtaggtat gggattccct aaacaagaag
     8161 aatggattat gacacatgaa aaccaatttg aatctacagt gatgatgggc gtaggtggtt
     8221 ctcttgaagt atttgctggg gctaaaaaga gagcgcctta tatctttaga aaattaaaca
     8281 ttgaatggat atatagagca ttaatagatt ggaaacgtat tggtagatta aagagtattc
     8341 caatatttat gtataaaata gccaaagcaa aaagaaaaat aaaaaaggcg aaataatcat
     8401 gatgacaaaa ataaaaccga ggaaatcctt aaatggagat tctcggtttt ttcggtttat
     8461 ttaataacga agcgggactc atcgagtttg tttctaaatt ctttttgttc ggctttggat
     8521 ttctttttaa aatcgttaag gaaagcttca tatttaggta atacatcatc aagttcaccg
     8581 taatctttta actttccgcc ttcaatccaa gcaatcttag tacaaaattg tctcacttgt
     8641 cctaagttat gactaacgaa aaagatggtt ttgttttgct ctttaaactc gtaaatttta
     8701 tctaaacatt tttgtgcaaa agtttggtca cctacagata aagcttcgtc aatgactaag
     8761 atatctggat taactgtgat attaattgaa aaaccaagtt ttgcacgcat accacttgaa
     8821 tactttttaa ctggttgata aataaactca ccaagttcac taaattcaat aatcttaggt
     8881 gtcatcgctt taatttcttt tcgcttaaag cccatacata acattttaaa ttcgatattt
     8941 tcaatccctg taagttgtcc actcaagcca gcactaattg cgataacgct gacttcacca
     9001 ttacgatcca ctttgccaac agtaggcgac aaagaaccgc caatgatatt gctcaacgtt
     9061 gatttgccgg aaccattgat gccaacaagc cctatgacgt caccttcata tgcttttaaa
     9121 ctaatgtcat ctaaagcgaa aaatgttttg tttttatgtt tgggaatgag cgcatctttc
     9181 atacgttctt tatttgtacg ataaatacga tattcttttg ttacattttt aatgtttacc
     9241 gaaacgttca tttgtagacc ttccttattc acatttatct agattataat atactactca
     9301 acagttgtta aattttaaaa cctgttgtaa agtgtataga agattttgtt attatcagag
     9361 tgggtgtttt gacacaaaat gttaatcatc aatgataaca atgatattta aaaactaaac
     9421 ttatttcaac ttacatgatt gtatactata atgtatttgt aataaactaa tattttaaag
     9481 aactagacaa taattttgat agcatccatg tatagtgata gtatttacaa caattattat
     9541 aatactattt agttaagtag agaaatagtt aaacatttga aagtgtggtt taatggaatg
     9601 tcagcaatag gaacagtttt taaagaacat gtaaagaact tttatttaat tcaaagactg
     9661 gctcagtttc aagttaaaat tatcaatcat agtaactatt taggtgtggc ttgggaatta
     9721 attaaccctg ttatgcaaat tatggtttac tggatggttt ttggattagg aataagaagt
     9781 aatgcaccaa ttcatggtgt accttttgtt tattggttat tggttggtat cagtatgtgg
     9841 ttcttcatca accaaggtat tttagaaggt actaaagcaa ttacacaaaa gtttaatcaa
     9901 gtatcgaaaa tgaacttccc gttatcgata ataccgacat atattgtgac aagtagattt
     9961 tatggacatt taggcttact tttacttgtg ataattgcat gtatgtttac tggtatttat
    10021 ccatcaatac atatcattca attattgata tatgtaccgt tttgtttttt cttaactgcc
    10081 tcggtgacgt tattaacatc aacactcggt gtgttagtta gagatacaca aatgttaatg
    10141 caagcaatat taagaatatt attttacttt tcaccaattt tgtggctacc aaagaaccat
    10201 ggtatcagtg gtttaattca tgaaatgatg aaatataatc cagtttactt tattgctgaa
    10261 tcataccgtg cagcaatttt atatcacgaa tggtatttca tggatcattg gaaattaatg
    10321 ttatacaatt tcggtattgt tgccattttc tttgcaattg gtgcgtactt acacatgaaa
    10381 tatagagatc aatttgcaga cttcttgtaa tatatttata tgacgaaacc ccgctaacca
    10441 ttaataaatg gaagtggggt tcatttttgt ttataattta agtaaataac atattaagtt
    10501 ggtgtattat gaacgtttta ataaagaaat tttatcattt ggtagttcga atactttcta
    10561 aaatgattac gcctcaagtg attgataaac cgcatatcgt atttatgatg acttttccag
    10621 aagatattaa gcctatcatc aaagcattaa ataattcgtc gtatcagaaa actgttttaa
    10681 caacaccaaa acaagcgcct tatttatctg aacttagcga cgatgttgat gtgatagaaa
    10741 tgactaatcg aacattggta aaacaaatta aggctttgaa aagcgcgcag atgattatta
    10801 tcgataatta ttacctatta ctaggtggat ataataagac ttctaatcaa cacattgttc
    10861 aaacgtggca tgcaagtggt gcattaaaaa actttggctt aacagatcat caagtcgatg
    10921 tgtctgacaa ggcaatggtt cagcagtacc gtaaagttta tcaagcgacg gatttttact
    10981 tagtgggttg tgaacaaatg tcacaatgtt ttaaacagtc tttaggtgca acagaagagc
    11041 aaatgctgta ttttgggctt ccgagaatta ataaatatta cacagctgat agagcaacgg
    11101 ttaaggcaga gttaaaggat aaatatggaa ttacaaataa gttggtatta tatgtaccaa
    11161 catatagaga agataaagca gataataggg ctattgataa agcttatttt gaaaaatgtt
    11221 taccaggata tacactgatt aataaattac atccatcaat tgaagattca gacattgatg
    11281 acgtatcttc aatcgacacg tctacattaa tgctaatgtc agatataatt attagcgact
    11341 atagttcgct gccaatagaa gctagcttgt tagatattcc aactatattt tatgtgtatg
    11401 atgaaggaac atatgatcag gtgagaggcc tgaatcaatt ttacaaagca ataccggata
    11461 gctacaaagt gtatactgaa gaagatttaa taatgacgat acaagaaaaa gaacatctat
    11521 taagtccgtt atttaaagat tggcataagt ataatactga taaaagttta catcagctca
    11581 cagaatatat agataagatg gtgacaaaat gaggtttacg ataatcatac ctacatgtaa
    11641 taatgaggca acaattcgac aattgttaat atctattgag agtaaagaac actatagaat
    11701 cctttgtatt gatggtggtt ctactgatca aacaattcct atgattgaac ggttacaaag
    11761 agaactcaag catatttcat taatacaatt acaaaatgct tcgatagcta cgtgtattaa
    11821 taaaggtttg atggatatca aaatgacaga tccacatgat agtgacgcat ttatggtcat
    11881 aaaaccaaca tcaatcgtat tgccaggtaa attagatagg ttaactgctg ctttcaaaaa
    11941 taatgataat attgatatgg taatagggca gcgagcttac aattaccatg gtgaatggaa
    12001 attgaaaagt gctgatgagt ttattaaaga caatcgaatc gttacattaa cggaacaacc
    12061 agatttgtta tcaatgatgt cttttgacgg aaagttattc agtgctaaat ttgctgaatt
    12121 acagtgtgac gaaactttag ctaacacata caatcacgca atacttgtca aggcgatgca
    12181 aaaagctacg gatatacatt tagtttcaca gatgattgtc ggagataacg atatagatac
    12241 acatgctaca agtaacgaag aagattttaa tagatatatc acagaaatta tgaaaataag
    12301 acaacgagtc atggaaatgt tactattacc tgaacaaagg ctattatata gtgatatggt
    12361 tgatcgtatt ttattcaata attcattaaa atattatatg aacgaacacc cagcagtaac
    12421 gcacacgaca attcaactcg taaaagacta tattatgtct atgcagcatt ctgattatgt
    12481 atcgcaaaac atgtttgaca ttataaatac agttgaattt attggtgaga attgggatag
    12541 agaaatatac gaattgtggc gacaaacatt aattcaagtg ggcattaata ggccgactta
    12601 taaaaaattc ttgatacaac ttaaagggag aaagtttgca catcgaacaa aatcaatgtt
    12661 aaaacgataa cgtgtacatt gatgaccata aactgcaatc ctatgatgtg acaatatgag
    12721 gaggataact taatgaaacg tgtaataaca tatggcacat atgacttact tcactatggt
    12781 catatcgaat tgcttcgtcg tgcaagagag atgggcgatt atttaatagt agcattatca
    12841 acagatgaat ttaatcaaat taaacataaa aaatcttatt atgattatga acaacgaaaa
    12901 atgatgcttg aatcaatacg ctatgtcgat ttagtcattc cagaaaaggg ctggggacaa
    12961 aaagaagacg atgtcgaaaa atttgatgta gatgtttttg ttatgggaca tgactgggaa
    13021 ggtgaattcg acttcttaaa ggataaatgt gaagtcattt atttaaaacg tacagaaggc
    13081 atttcgacga ctaaaatcaa acaagaatta tatggtaaag atgctaaata aattatatag
    13141 aactatcgat actaaacgat aaattaactt aggttattat aaaataaata taaaacggac
    13201 aagtttcgca gctttataat gtgcaacttg tccgttttta gtatgtttta ttttcttttt
    13261 ctaaataaac gattgattat catatgaaca ataagtgcta atccagcgac aaggcatgta
    13321 ccaccaatga tagtgaataa tggatgttct tcccacatac ttttagcaac agtatttgcc
    13381 ttttgaataa ttggctgatg aacttctaca gttggaggtc cataatcttt attaataaat
    13441 tctcttggat agtccgcgtg tactttacca tcttcgacta caagtttata atctttttta
    13501 ctaaaatcac ttggtaaaac atcgtaaaga tcattttcaa cataatattt cttaccattt
    13561 atcctttgct cacctttaga caatattttt acatatttat actgatcaaa tgagcgttcc
    13621 attaatgcat tccccatcat attacgttgc ttctcgccac caaggttttt atagtctcct
    13681 gcacccatga taacttgatt aattctaaat ttacctcgtt tggtagtaat cgtatggttg
    13741 taatttgctg tatcacttga tccagttttt aaaccatctg tacccggcaa actcattttt
    13801 gcaccttcca atgaaaagtt gaatgtgtaa tacgtaactg catgcgttgt tggtgctaac
    13861 tgctttgtaa agtctaatat tttaggtgtc tctttaatca cgtgtaaatc taaaatggca
    13921 tagtctctag cagtcgttac agtacgttct tggtctttat actttgttgg tgcaaatgta
    13981 cgtaatcttg aattttcagc acccgttgga ttgacgaaat gtgtattttt cattccgata
    14041 gctttagctt tgttattcat taaatcaacg aaatcgctgg tgttttttga aaccttctta
    14101 gctaaaatta atgccgcggc attactagaa ttagatactg taatttgtaa taggtctgcg
    14161 attgtccata cttgtccagg atatagtttc gtattactca actcaggtag tgtagacata
    14221 atatattctt tgttcgtcat tgtgactgtg tcatcaagtg aaagctgccc cttatttaca
    14281 gcttccaatg ttaagtacat tgtcattaat ttagtcatag acgctggatt ccacttagta
    14341 tcgatattgt attgatacag taattgtcca gtttgactta cattaacagc actcgtcggt
    14401 tcgtatgcag ccgacaaacc tgcataacca tattgatttg ctgcttgtac aggggttacg
    14461 tcactgttag tagcttgtgc atatggtgtc ataatactta atgttaaaca taaaatgatg
    14521 ataatagata ttaaattttt cataaagcgt taatcttccc ttttccaatt cttaaatatt
    14581 ccctaaaagc aatggttatt cctacttacg gaaatcattg ctaattcact tcaccttaat
    14641 taaattgttg aaaataaagt tttctgcagt taatttgaaa aataatgcaa atatattacg
    14701 tgtgtagcta aaggtgttat aatgtttgta cgaagagcaa acttactcaa aagcgattaa
    14761 ttttcatgtt ttaatataaa gactttgaga agttattaca aaaaatgcaa tagaaatatt
    14821 ctatcatata aatgttatga gcggtatttt ggggcaacac tttatttgat ttttaaagtt
    14881 ttgttgggag aaagtatatg atagaaatgc atgtatctat ctaaatgaat taactataaa
    14941 tttcaaacag aagaggtaaa actatgaaac gagaaaatcc attgtttttc ttatttaaaa
    15001 aactatcatg gccagtgggt cttatcgttg cagctatcac tatttcatca ctagggagct
    15061 taagtggact attagtgcca ctgtttactg gacgaattgt agataaattt tccgtgagcc
    15121 atatcaattg gaatctaatc gcattatttg gtggtatctt tgtcatcaat gctttattaa
    15181 gcggattagg tttatattta ttaagtaaaa ttggtgaaaa gattatttat gcgatacgct
    15241 cagttttatg ggagcatatc atacaattaa aaatgccatt ctttgacaaa aatgaaagtg
    15301 gtcaattaat gagtcgatta actgacgata cgaaagtgat aaatgaattt atttcacaaa
    15361 agctacctaa cttattacca tcaatcgtta cattagttgg gtcactaatc atgttattta
    15421 ttttagattg gaaaatgaca ttattaacat ttataacgat accgatattc gttttaatta
    15481 tgattcctct aggtcgtatt atgcaaaaga tatcgacaag tacacaatct gaaattgcaa
    15541 acttcagtgg tttgttaggg cgtgtcctaa ctgaaatgcg tcttgttaaa atatcaaata
    15601 cagagcgtct tgaattagat aatgcacata aaaatttgaa tgaaatatat aaattaggtt
    15661 taaaacaggc taaaattgcg gcagttgtac aaccaatttc aggtatagtt atgttgctaa
    15721 caattgcaat tattttaggt tttggtgcat tagaaattgc gactggtgca atcactgcag
    15781 gtacattaat tgcaatgata ttttatgtta ttcagttatc tatgccttta atcaatcttt
    15841 ccacgttagt tacagattat aaaaaggcag tcggtgcaag tagtagaata tacgaaatca
    15901 tgcaagaacc tattgaaccg acagaagctc ttgaagattc tgaaaatgta ttaattgatg
    15961 acggtgtatt gtcatttgaa catgtagact ttaaatatga tgtgaagaaa atattagatg
    16021 atgtgtcgtt ccaaatccca caaggtcaag tgagtgcttt tgtaggccct tctgggtctg
    16081 gtaaaagtac gatatttaat ctgatagaac gtatgtatga aattgagtca ggtgatatta
    16141 aatatggcct tgaaagtgtc tatgatatcc cgttatctaa gtggcgacgc aaaattggat
    16201 atgttatgca atcaaattcg atgatgagtg gtacaattag agacaatatt ttatacggaa
    16261 ttaatcgtca tgtttcagat gaagaactta ttaattatgc taaattagcg aactgtcatg
    16321 attttatcat gcaatttgat gaaggatatg acacgcttgt aggtgaacga ggattgaaac
    16381 tgtctggcgg acaacgtcaa cgtattgata ttgctagaag ttttgttaaa aatcctgata
    16441 ttttgttact tgatgaagca acagctaatc tcgatagtga aagtgaattg aaaattcaag
    16501 aagctttaga aacattgatg gaaggtagaa caacgattgt cattgcgcat cgtttgtcta
    16561 caattaaaaa agccggtcaa attatattct tagacaaagg acaggtaaca ggtaaaggta
    16621 cgcattcaga actgatggca tcacatgcga agtataaaaa ctttgtagtg tctcaaaaat
    16681 taacagatta attttatata tataagtaag cttggagcaa atacacatat accatcgagg
    16741 aaattaaagt gtggcacatt gatggatata gatgttaata aattgcttca agcttttgtc
    16801 tattttaaat catttgagaa gttacgacat aataattctt aaattaatga aatcgatatt
    16861 ttaagaaaaa aatgctcatg gtataataca agttataagc aaacatacat atattaaata
    16921 ctgtagccac gagtcataat tcttcgtatt ttacatagca atttaactga ttttagagtc
    16981 cacggtacag aagtttgata tttcaatgtt tctaaatttt taaaaaatta aatcataggt
    17041 gggtgccaaa tgtttttatt aatcaacatt attggtctaa ttgtatttct tggtattgcg
    17101 gtattatttt caagagatcg caaaaatatc caatggcaat caattgggat cttagttgtt
    17161 ttaaacctgt ttttagcatg gttctttatt tattttgatt ggggtcaaaa agcagtaaga
    17221 ggagcagcca atggtatcgc ttgggtagtt cagtcagcgc atgctggtac aggttttgca
    17281 tttgcaagtt tgacaaatgt taaaatgatg gatatggctg ttgcagcctt attcccaata
    17341 ttattaatag tgccattatt tgatatctta atgtacttta atattttacc gaaaattatt
    17401 ggaggtattg gttggttact agctaaagta acaagacaac ctaaattcga gtcattcttt
    17461 gggatagaaa tgatgttctt aggaaatact gaagcattag ccgtatcaag tgagcaacta
    17521 aaacgtatga atgaaatgcg tgtattaaca atcgcaatga tgtcaatgag ctctgtatcg
    17581 ggagctattg taggtgcgta tgtacaaatg gtaccaggag aactggtact aacggcaatt
    17641 ccactaaata tcgttaacgc gattattgtg tcatgcttgt tgaatccagt aagtgttgaa
    17701 gagaaagaag atattattta cagtcttaaa aacaatgaag ttgaacgtca accattcttc
    17761 tcattccttg gagattctgt attagcagca ggtaaattag tattaatcat catcgcattt
    17821 gttattagtt ttgtagcgtt agctgatcta tttgatcgtt ttatcaattt gattacagga
    17881 ttgatagcag gatggatagg cataaaaggt agtttcggtt taaaccaaat tttaggtgtg
    17941 tttatgtatc catttgcgct attactcggt ttaccttatg atgaagcgtg gttggtagca
    18001 caacaaatgg ctaagaaaat tgttacaaat gaatttgttg ttatgggtga aatttctaaa
    18061 gatattgcat cttatacacc acaccatcgt gcggttatta caacattctt aatttcattt
    18121 gcaaacttct caacgattgg tatgattatc ggtacattga aaggcattgt tgataaaaag
    18181 acatcagact ttgtatctaa atatgtacct atgatgctat tatcaggtat cctagtttca
    18241 ttattaacag cagctttcgt tggtttattt gcatggtaat atgtcgaaga gtgactatga
    18301 taatacattt taactaataa atatgtccag gcatgtcgtc tattgatata ggtgagatgc
    18361 ttggactttt ttattattga tataaaggta tttaaatatt tttaaagtta ccgaaattga
    18421 agcattataa aaaccaagtg cacatggtaa tacacttggc ttttatggga aatgaatatt
    18481 attgtacata tgacagtaag gactaggtac agtcatagta cttcgagcaa aatttgtttt
    18541 gttattataa acaacacaaa ggagataact tctctattga agaagttaaa aacattatag
    18601 cagacaatga aatgaaagta aattaaaaat tcagaatatt tttaattatt atattgtgag
    18661 tgatatttat tagggaaagc tattcttcat ataaattagt taaatagtaa atctttgtta
    18721 gaactctttc catagagttg tgacagcatt tttgttgtgc tactattttt atagtctaat
    18781 aaatatataa aggggatggt ttcgtgaata aaacggttaa agatttaata ctagttgtct
    18841 taggttcatt tatctttgct gcaggtgtaa atgcatttat tatttctggt aacttaggtg
    18901 aaggcggggt tacaggttta gcaattattt tatattatgc gtttcatatt tcaccagcca
    18961 tcactaactt cttggtcaac gcagtattga ttgccatagg ttataaattt ttgagtaaga
    19021 gaagtatgta cttaactatt cttgtaacaa ttcttatttc aatatttttg agtttaacag
    19081 aatcatggca agtagaaact ggaaacagca ttgtgaatgc catttttggt ggtgtaagcg
    19141 ttggactagg aatcggagta attatccttg caggcggtac aacagcaggt acaacaattt
    19201 tggcgagaat tgcaacgaaa tacctcgatg taagcacgcc atatgctttg cttttcttcg
    19261 atatgatcgt tgttgcaatt tcacttacag ttattccact tgataaagta ttagtaacag
    19321 taatatcact ttatatagga acaaaagtga tggaatatgt catagaaggt ttaaacacta
    19381 aaaaagctat gacgattatt tcaactaatc ccgacaaact tgccaaagca atagacgagc
    19441 aaattggaag aggtttaacc attttaaacg gacatggcta ttatacgcgt gaagaaaaag
    19501 atgtcttata cgttgttatt tctaaaacac aagtttcaaa agcaaagcga ttaattaaac
    19561 aaatcgataa agatgcattc ctcgtaattc atgatgtaag agatgtctat ggtaatggct
    19621 ttcttgcaga tgaataaata aatggtatga gcacacatac ttaaatagaa gtccacggac
    19681 aagtttttga actatgaaga cttatctgtg ggcgtttttt attttataaa agtaatatac
    19741 aagacatgac aaatcgagct atccaattta aaaagtaatg ttagtcaata agattgaaaa
    19801 atgttataat gatgttcatg ataatcatta tcaattggga tgtctttgaa aattgataat
    19861 ttaaaaatag aaattatttt ttataaacag aaagaatttt attgaaagta gggaaattat
    19921 gaatcgtttg catggacaac aagttaaaat tggttacggg gataacacga ttataaataa
    19981 attagatgtt gaaataccag atggcaaagt gacgtcaatc attggtccta acggctgcgg
    20041 gaaatctact ttgctaaagg cattgtcacg tttattggca gttaaagaag gcgaagtatt
    20101 tttagatggt gaaaatattc atacacaatc tacgaaagag attgcaaaaa aaatagccat
    20161 tttacctcaa tcacctgaag tagcagatgg cttaactgtt ggggaattag tttcatatgg
    20221 tcgttttcca catcaaaaag gatttggtag attaactgct gaggataaga aagaaattga
    20281 ttgggcaatg gaagttacag gaactgatac attccgacac cgttcaatca atgatttaag
    20341 tggtggtcaa agacaacgtg tttggattgc aatggcatta gcacaaagaa ctgatattat
    20401 ctttttagac gaaccaacaa catatttaga tatctgtcat caattagaaa tactagaatt
    20461 agttcagaag ctaaatcagg aacaaggttg tacaattgtc atggttcttc atgatatcaa
    20521 ccaagcgatt cgtttctcag atcatcttat tgcgatgaaa gaaggggata tcatcgctac
    20581 aggttcaaca gaagacgtat taacacagga aatattagaa aaagttttta atattgatgt
    20641 tgttttaagt aaagatccta aaactggaaa acctttactg gtaacttatg acttatgtcg
    20701 cagagcttat tcttaattaa gtaagttaat atgataaaaa ggacaattaa catgacaaat
    20761 agagagaacc caacgccatt gaagttttta tcctatatta taggtttaag tatgatacta
    20821 ctaatcacac tatttatttc tacattaata ggtgacgcca aaattcaagc ctctacaatt
    20881 atagaggcta tttttaatta taatcctagc aatcaacagc aaaacatcat caatgagatt
    20941 aggattccca gaaatatagc agcagtaatt gtaggtatgg cgcttgcagt ttctggtgcg
    21001 attatacaag gtgttactcg taatggtctt gctgatccgg cgctcatagg tttaaattca
    21061 ggtgcttcat ttgctttagc attaacatat gcagttttac caaacacttc atttttaata
    21121 ttgatgtttg ctggattttt aggtgctatt ctaggaggtg ctattgtatt aatgataggc
    21181 cgatctagac gtgatggatt taatccgatg cgtattattt tagcgggtgc agcagtaagt
    21241 gctatgttaa cagcgctaag tcaaggtatt gcattagctt ttagactaaa tcaaacagta
    21301 acattttgga ctgctggagg cgtttcaggc acaacatggt cacaccttaa gtgggcaatt
    21361 ccattaattg gtattgcgtt attcattata ttaacaatta gtaaacaact taccatttta
    21421 aatcttggtg aatcattagc taaaggttta ggtcaaaatg taacaatgat cagaggcata
    21481 tgtttaatta ttgctatgat tctagcaggt attgcagttg ctatcgctgg acaagttgca
    21541 tttgtaggtt tgatggtacc tcatatagca agatttttaa ttggaactga ttatgctaaa
    21601 attctaccat taacagcctt gttaggtggg atactcgtgc ttgttgccga tgtgatagca
    21661 cgatatttag gagaagcgcc tgttggtgca atcatttcat ttatcggtgt tccttacttt
    21721 ttatatttag ttaaaaaagg aggacgctca atatgattag ttcaaataat aaacgcagac
    21781 aattgatagc actggctgtt tttagcattc tactatttct aggttgtact tggagtatta
    21841 cctcaggtga atacaacata cctgttgaaa gatttttcaa aactttaatt ggacaaggtg
    21901 atgccattga taagttaatc ttattagatt tcaggttacc tcggatgatg attactattt
    21961 tggctggcgc agcgcttagt attagtggtg caatagtgca aagtgtcaca aaaaatccaa
    22021 tagctgaacc aggtatatta ggtattaacg caggtggcgg atttgcaatc gcattattta
    22081 ttgcaattgg taaaattaat gctgacaact ttgtttatgt actgccgtta ataagtatac
    22141 taggtggtat caccactgca ttgattattt ttattttcag ttttaataaa aatgaaggtg
    22201 ttacacctgc gagtatggta ttaataggtg taggtttaca aacagcatta tatggtggct
    22261 caattacaat tatgtcaaaa tttgatgata agcaatctga tttcatcgct gcttggtttg
    22321 caggtaatat ttggggtgac gaatggccat ttgtcattgc atttttaccg tgggtgttga
    22381 ttattattcc ttacttacta tttaaatcga atacactaaa tattattcat acgggtgata
    22441 atattgcacg aggtctaggt gtaaggttaa gcagagaacg tttaatatta ttctttatcg
    22501 cagtgatgtt atcatctgct gctgtagcag tagcaggttc aatttcgttt atcggattaa
    22561 tgggtccgca tattgccaaa cgtatcgttg gaccacgtca ccagttgttt ttaccaattg
    22621 ccattttagt aggggcatgt ttacttgtta tagctgatac aattggcaaa attgtattac
    22681 aaccaggtgg ggttccagca ggtattgtcg tagcaattat tggtgcaccg tatttcttat
    22741 atttaatgta caaaacgaaa aatgtatagt gtcaatggac acaacttatt gctatgaaag
    22801 gcactttatt ataaggcttt tcatagcatt ttttatttaa tgagccactc aagactattt
    22861 attttttcaa taatgaacca ttaagttatc aagaggatct tat
//
LOCUS       DAKTIO010000021        22898 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.021, whole genome shotgun sequence.
ACCESSION   DAKTIO010000021 DAKTIO010000000
VERSION     DAKTIO010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22898
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.021"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(100..1098)
                     /gene="sirC"
                     /locus_tag="O0293_000651"
     CDS             complement(100..1098)
                     /gene="sirC"
                     /locus_tag="O0293_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirC"
                     /protein_id="HCY0279550.1"
                     /translation="MTEKINKKDNYHLIFALIFLAIVSVVSMMIGSSFIPLQRVLMYF
                     INPNDSMDQFTLEVLRLPRITLAILAGAALGMSGLMLQNVLKNPIASPDIIGITGGAS
                     LSAVVFIAFFSHLTIHLLPLFAVLGGAVAMMILLVFQTKGQIRPTTLIIIGISMQTLF
                     IALVQGLLITTKQLSAAKAYTWLVGSLYGATFKDTIILGMVILAVVPLLFLVIPKMKI
                     SILDDPVAIGLGLHVQRMKLIQLITSTILVSMAISLVGNIGFVGLIAPHIAKTIVRGS
                     YAKKLLMSAMIGAISIVIADLIGRTLFLPKEVPAGVFIAAFGAPFFIYLLLTVKKL"
     gene            complement(1095..2090)
                     /gene="sirB"
                     /locus_tag="O0293_000652"
     CDS             complement(1095..2090)
                     /gene="sirB"
                     /locus_tag="O0293_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirB"
                     /protein_id="HCY0279551.1"
                     /translation="MLLKPKYQIVIAGLCLAIVAILSLMIGNTLVSPGTVIQALFNFD
                     SENDLHDVVTGARASRTIIALLTGAALAVSGLLMQALTRNPIASPGLFGVNAGAVFFV
                     IFSITFIQIQSFKMIVVIAFLGAIVVTVLVVALGMFRQTLFSPHRVILAGAAIAMLFT
                     AFTQGILIMNETDLQGLLFWLSGSVSLRNIWDIPWIIPLVLILILIAFSMAAHINILM
                     TSDDIATGLGQNIKLIKWMIIMLISMLAGISVAVAGSIVFVGLIVPNISKRLLPPNYK
                     YLIPFTALAGAILMIISDIVARIIIKPLELPIGVVTAVIGAIVLIYIMKKGRQRL"
     gene            complement(2106..3098)
                     /gene="sirA"
                     /locus_tag="O0293_000653"
     CDS             complement(2106..3098)
                     /gene="sirA"
                     /locus_tag="O0293_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter
                     substrate-binding protein SirA"
                     /protein_id="HCY0279552.1"
                     /translation="MNKVIKMLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAM
                     GTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVESWTQKPKFEYIKNDLKDTKIVGQ
                     EPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALG
                     KEKEAEDLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILN
                     DLGFKRNKDLQKQVDNGKDIIQLTSKESIPLMNADHIFVVKSDPNAKDAALVKKTESE
                     WTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLYEKLNIEKQSK"
     gene            3329..4309
                     /gene="sbnA"
                     /locus_tag="O0293_000654"
     CDS             3329..4309
                     /gene="sbnA"
                     /locus_tag="O0293_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="HCY0279553.1"
                     /translation="MIEKSQACHDSLLDSVGQTPMVQLHQLFPKHEVFAKLEYMNPGG
                     SMKDRPAKYIIEHGIKHGLITENTHLIESTSGNLGIALAMIAKIKGLKLTCVVDPKIS
                     PTNLKIIKSYGANVEMVEEPDAHGGYLMTRIAKVQELLATIDDAYWINQYANELNWQS
                     HYHGAGTEIVETIKQPIDYFVAPVSTTGSIMGMSRKIKEVHPNAQIVAVDAKGSVIFG
                     DKPINRELPGIGASRVPEILNRSEINQVIHVDDYQSALGCRKLIDYEGIFAGGSTGSI
                     IAAIEQLITSIEEGATIVTILPDRGDRYLDLVYSDTWLEKMKSRQGVKSE"
     gene            4306..5316
                     /gene="sbnB"
                     /locus_tag="O0293_000655"
     CDS             4306..5316
                     /gene="sbnB"
                     /locus_tag="O0293_000655"
                     /EC_number="1.5.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate
                     dehydrogenase SbnB"
                     /protein_id="HCY0279554.1"
                     /translation="MNREMLYLNRSDIEQAGGNHSQVYVDALTEALTAHAHNDFVQPL
                     KPYLRQDPENGHIADRIIAMPSHIGGEHAISGIKWIGSKHDNPSKRNMERASGVIILN
                     DPETNYPIAVMEASLISSMRTAAVSVIAAKHLAKKGFKDLTIIGCGLIGDKQLQSMLE
                     QFDHIERVFVYDQFSEACARFVDRWQQQRPEINFIATENAKEAVSNGEVVITCTVTDQ
                     PYIEYDWLQKGAFISNISIMDVHKEVFIKADKVVVDDWSQCNREKKTINQLVLEGKFS
                     KEALHAELGQLVTGDIPGREDDDEIILLNPMGMAIEDISSAYFIYQQAQQQNIGTTLN
                     LY"
     gene            5337..7091
                     /gene="sbnC"
                     /locus_tag="O0293_000656"
     CDS             5337..7091
                     /gene="sbnC"
                     /locus_tag="O0293_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis protein SbnC"
                     /protein_id="HCY0279555.1"
                     /translation="MQNHTAVNTAQAIILRDLVDALLFEDIAGIVSNSEITKENGQTL
                     LIYERETQQIKIPVYFSALNMFRYESSQPITIEGRVSKQPLTAAEFWQTIANMNCDLS
                     HEWEVARVEEGLTTAATQLAKQLSELDLASHPFVMSEQFASLKDRPFHPLAKEKRGLR
                     EADYQVYQAELNQSFPLMVAAVKKTHMIHGDTANIDELENLTVPIKEQATDMLNDQGL
                     SIDDYVLFPVHPWQYQHILPNVFAKEISEKLVVLLPLKFGDYLSSSSMRSLIDIGAPY
                     NHVKVPFAMQSLGALRLTPTRYMKNGEQAEQLLRQLIEKDEALAKYVMVCDETAWWSY
                     MGQDNDIFKDQLGHLTVQLRKYPEVLAKNDTQQLVSMAALAANDRTLYQMICGKDNIS
                     KNDVMTLFEDIAQVFLKVTLSFMQYGALPELHGQNILLSFEDGRVQKCVLRDHDTVRI
                     YKPWLTAHQLSLPKYVVREDTPNTLINEDLETFFAYFQTLAVSVNLYAIIDAIQDLFG
                     VSEHELMSLLKQILKNEVATISWVTTDQLAVRHILFDKQTWPFKQILLPLLYQRDSGG
                     GSMPSGLTTVPNPMVTYD"
     gene            7084..8340
                     /gene="sbnD"
                     /locus_tag="O0293_000657"
     CDS             7084..8340
                     /gene="sbnD"
                     /locus_tag="O0293_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B export MFS transporter"
                     /protein_id="HCY0279556.1"
                     /translation="MINQSIWRSNFRILWLSQFIAIAGLTVLVPLLPIYMASLQNLSV
                     VEIQLWSGIAIAAPAVTTMIASPIWGKLGDKISRKWMVLRALLGLAVCLFLMALCTTP
                     LQFVLVRLLQGLFGGVVDASSAFASAEAPAEDRGKVLGRLQSSVSAGSLVGPLIGGVT
                     ASILGFSALLMSIAVITFIVCIFGALKLIETTHMPKSQTPNINKGIRRSFQCLLCTQQ
                     TCRFIIVGVLANFAMYGMLTALSPLASSVNHTAIDDRSVIGFLQSAFWTASILSAPLW
                     GRFNDKSYVKSVYIFATIACGCSAILQGLATNIEFLMAARILQGLTYSALIQSVMFVV
                     VNACHQQLKGTFVGTTNSMLVVGQIIGSLSGAAITSYTTPATTFIVMGVVFAVSSLFL
                     ICSTITNQINDHTLMKLWELKQKSAK"
     gene            8330..10066
                     /gene="sbnE"
                     /locus_tag="O0293_000658"
     CDS             8330..10066
                     /gene="sbnE"
                     /locus_tag="O0293_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,3-diaminopropanoate--citrate ligase SbnE"
                     /protein_id="HCY0279557.1"
                     /translation="MQNKELIQHAAYAAIERILNEYFREENLYQVPPQNHQWSIQLSE
                     LETLTGEFRYWSAMGHHMYHPEVWLIDGKSKKITTYKEAIARILQHMAQSADNQTAVQ
                     QHMAQIMSDIDNSIHRTARYLQSNTIDYVEDRYIVSEQSLYLGHPFHPTPKSASGFSE
                     ADLEKYAPECHTSFQLHYLAVHQDVLLTRYVEGKEDQVEKVLYQLADIDISEIPKDFI
                     LLPTHPYQINVLRQHPQYMQYSEQGLIKDLGVSGDSVYPTSSVRTVFSKALNIYLKLP
                     IHVKITNFIRTNDLEQIERTIDAAQVIASVKDEVETPHFKLMFEEGYRALLPNPLGQT
                     VEPEMDLLTNSAMIVREGIPNYHADKDIHVLASLFETMPDSPMSKLSQVIEQSGLAPE
                     AWLECYLNRTLLPILKLFSNTGISLEAHVQNTLIELKDGIPDVCFVRDLEGICLSRTI
                     ATEKQLVPNVVAASSPVVYAHDEAWHRLKYYVVVNHLGHLVSTIGKATRNEVVLWQLV
                     AHRLMTWKKEYANNAVFVDCVEDLYQTPTIAAKANLMSKLNDCGANPIYTHIPNPICH
                     NKEVSYCESNNS"
     gene            9975..11825
                     /gene="sbnF"
                     /locus_tag="O0293_000659"
     CDS             9975..11825
                     /gene="sbnF"
                     /locus_tag="O0293_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(L-alanin-3-ylcarbamoyl)-2-[(2-
                     aminoethylcarbamoyl)methyl]-2-hydroxypropanoate synthase
                     SbnF"
                     /protein_id="HCY0279558.1"
                     /translation="MIVVQTLFIHIYQIQFVITRRYRIVNQTILNRVKTRVMHQLVSS
                     LIYENIVVYKASYQDGVGHFTIEGHDSEYRFTAEKTHSFDRIRITSPIERVVGDEADT
                     TTDYTQLLREVVFTFPKNDEKLEQFIVELLQTELKDTQSMQYRESNPPATPETFNDYE
                     FYAMEGHQYHPSYKSRLGFTLSDNLKFGPDFVPNVKLQWLAIDKDKVETTVSRNVVVN
                     EMLRQQVGDKTYEHFVQQIEASGKHVNDVEMIPVHPWQFEHVIQVDLAEERLNGTVLW
                     LGESDELYHPQQSIRTMSPIDTTKYYLKVPISITNTSTKRVLAPHTIENAAQITDWLK
                     QIQQQDMYLKDELKTVFLGEVLGQSYLNTQLSPYKQTQVYGALGVIWRENIYHMLIDE
                     EDAIPFNALYASDKDGVPFIENWIKQYGSEAWTKQFLAVAIRPMIHMLYYHGIAFESH
                     AQNMMLIHENGWPTRIALKDFHDGVRFKREHLSEAASHLTLKPMPEAHKKVNSNSFIE
                     TDDERLVRDFLHDAFFFINIAEIILFIEKQYGIDEELQWQWVKGIIEAYQEAFPELNN
                     YQHFDLFEPTIQVEKLTTRRLLSDSELRIHHVTNPLGVGGINDATTISET"
     gene            11800..12576
                     /gene="sbnG"
                     /locus_tag="O0293_000660"
     CDS             11800..12576
                     /gene="sbnG"
                     /locus_tag="O0293_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis citrate synthase
                     SbnG"
                     /protein_id="HCY0279559.1"
                     /translation="MQQLSLKHRLNNGDSVYGIFNSIPDPLMIEVIAASGYDFVVIDT
                     EHVAINDETLAHLIRAAEAAHIIPIVRVTAVIDRDIIKVLDMGARGIIVPHVKDRETV
                     EHIVKLSRYYPQGLRSLNGGRMARFGRTPLLDAMEMANEHIMVIAMIEDVEGVMAIDD
                     IAQVEGLDMIVEGAADLSQSLGIPWQTRDDQVTSHVQHIFEVVNAHGKHFCALPREDE
                     DIAKWQAQGVQTFILGDDRGKIYRHLSASLATSKQKGDEG"
     gene            12576..13778
                     /gene="sbnH"
                     /locus_tag="O0293_000661"
     CDS             12576..13778
                     /gene="sbnH"
                     /locus_tag="O0293_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis decarboxylase
                     SbnH"
                     /protein_id="HCY0279560.1"
                     /translation="MRIVQPVIEQLKAQSHPVCHYIYDLVGLEHHLQHITSSLPSNCQ
                     MYYAMKANSERKILDTISQYVEGFEVASQGEIAKGLAFKPANHIIFGGPGKTDEELRY
                     AVSEGVQRIHVESMHELQRLNAILEDEDKTQHILLRVNLAGPFPNATLHMAGRPTQFG
                     ISEDEVDDVIEAALAMPKIHLDGFHFHSISNNLDSNLHVDVVKLYFKKAKAWSEKHRF
                     PLKHINLGGGIGVNYADLTNQFEWDNFVERFKTLIVEQEMEDVTLNFECGRFIVAHIG
                     YYVTEVLDIKKVHGAWYAILRGGTQQFRLPVSWQHNHPFDIYRYKDNPYSFEKVSISR
                     QDTTLVGQLCTPKDVFAREVQIDAISTGDVIVFKYAGAYGWSISHHDFLSHPHPEFIY
                     LTQTKEDE"
     gene            13782..14546
                     /gene="sbnI"
                     /locus_tag="O0293_000662"
     CDS             13782..14546
                     /gene="sbnI"
                     /locus_tag="O0293_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional
                     regulator/O-phospho-L-serine synthase SbnI"
                     /protein_id="HCY0279561.1"
                     /translation="MNHIHEHLKLVPVDKIDLHETFEPLRLEKTKSSIEADDFIRHPI
                     LVTAMQHGRYMVIDGVHRYTSLKALGCKKVPVQEIHETQYSISTWQHKVPFGVWWETL
                     QQEHRLPWTTETRQEAPFITMCHGDTEQYLYTKDLGEAHFQVWEKVVASYSGCCSVER
                     IAQGTYPCLSQQDVLMKYQPLSYKEIEAVVHKGETVPAGVTRFNISGRCLNLQVPLAL
                     LKQDDDVEQLRNWKQFLADKFANMRCYTEKVYLVEQ"
     gene            14742..15149
                     /locus_tag="O0293_000663"
     CDS             14742..15149
                     /locus_tag="O0293_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279562.1"
                     /translation="MSSIIGKIAIWIGIVAQIYFSVVFVRMISINIAGGSDYETIFLL
                     GLILALFTVLPTIFTAIYMESYSVIGGALFIVYAIIALCLYNFLSSILWLIGGILLIW
                     NKYSKDESTDENEKVDIESTENQFESKDKITKE"
     gene            15199..15825
                     /locus_tag="O0293_000664"
     CDS             15199..15825
                     /locus_tag="O0293_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0279563.1"
                     /translation="MEKNVEKSFIKIGLYFQIAYIVLMAITLCGFVICYGLIFGLFYL
                     LSGSRADYLIVTIVISAIISIFVIILSIVPVIVLASDLFKERISKGVILIVLAIIALV
                     LCNFVSAILWFVSAISILGRKKLVAAADTTTIQKSKGNANQASHKDTCKKELDSQDMM
                     EHPEVKNPTTKNLEGFNEEIHKDEATTKVVSDNTEPPIESKDHVSKKD"
     gene            16037..16813
                     /locus_tag="O0293_000665"
     CDS             16037..16813
                     /locus_tag="O0293_000665"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HCY0279564.1"
                     /translation="MTNNKVALVTGGAQGIGFKIAERLVEDGFKVAVVDFNEEGAKAA
                     ALKLSSDGTKAIAIKADVSNRDDVFNAVRQTAAQFGDFHVMVNNAGLGPTTPIDTITE
                     EQFKTVYGVNVAGVLWGIQAAHEQFKKFNHGGKIINATSQAGVEGNPGLSLYCSTKFA
                     VRGLTQVAAQDLASEGITVNAFAPGIVQTPMMESIAVATAEEAGKPEAWGWEQFTSQI
                     ALGRVSQPEDVSNVVSFLAGKDSDYITGQTIIVDGGMRFR"
     gene            16957..17079
                     /locus_tag="O0293_000666"
     CDS             16957..17079
                     /locus_tag="O0293_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279565.1"
                     /translation="MHQLKALLVLTHPRYYKTSQKHHYLIYLNKNSQSYLILFL"
     gene            17149..18129
                     /locus_tag="O0293_000667"
     CDS             17149..18129
                     /locus_tag="O0293_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCY0279566.1"
                     /translation="MEDLERVLITGGAGFIGSHLVDDLQQDYDVYVLDNYRTGKRENI
                     KSLADDHVFELDIREYDAVEQIMKTYQFDYVIHLAALVSVAESVEKPILSQEINVVAT
                     LRLLEIIKKYNNHIKRFIFASSAAVYGDLPDLPKSDQSLILPLSPYAIDKYYGERTTL
                     NYCSLYNIPTAVVKFFNVFGPRQDPKSQYSGVISKMFDSFEHNKPFTFFGDGLQTRDF
                     VYVYDVVQSVRLIMEHKDAIGHGYNIGTGTFTNLLEVYRIIGELYGKSVEHEFKEARK
                     GDIKHSYADISNLKALGFVPKYTVETGLKDYFNFEVDNIEEVTAKEVEMS"
     gene            18092..18784
                     /locus_tag="O0293_000668"
     CDS             18092..18784
                     /locus_tag="O0293_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCY0279567.1"
                     /translation="MKKLQLKKWKCRENDIEAVHNNKGYAYQRKLDKLEEVRKSYYPI
                     KRAIDLILSIVLLFLTLPIMVIFAIAIVIDSPGNPIYSQVRVGKMGKLIKIYKLRSMC
                     KNAEKNGAQWADKDDDRITNVGKFIRKTRIDELPQLINVVKGEMSFIGPRPERPEFVE
                     LFSSEVIGFEQRCLVTPGLTGLAQIQGGYDLTPQQKLKYDMKYIHKGSLMMELYISIR
                     TLMVVITGEGSR"
     gene            18994..20160
                     /locus_tag="O0293_000669"
     CDS             18994..20160
                     /locus_tag="O0293_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCY0279568.1"
                     /translation="MKIIYCITKADNGGAQTHLIQLANHFCVHNDVYVIVGNHGPMIE
                     QLDARVNVIIIEHLVGPIDFKQDILAVKVLAQLFSKIKPDVIHLHSSKAGTVGRIAKF
                     ISKSKDTRIVFTAHGWAFTEGVKPAKKFLYLVIEKLMSLITDSIICVSDFDKQLALKY
                     RFNRLKLTTIHNGIADVPAVKQTLKSQSHNNIGEVVGMLPNKQDLQINAPTKHQFVMI
                     ARFAYPKLPQNLIAAIEILKLHNSNHAHFTFIGDGPTLNDCQQQVVQAGLENEVTFLG
                     NVINASHLLSQYDTFILISKHEGLPISIIEAMATGLPVIASHVGGISELVADNGICMM
                     NNQPETIAKVLEKYLIDSDYIKMSNQSRKRYLECFTEEKMIKEVEDVYNGKSTQ"
     gene            20141..21379
                     /locus_tag="O0293_000670"
     CDS             20141..21379
                     /locus_tag="O0293_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCY0279569.1"
                     /translation="MENQHNSKLLTLLLIGLAVFIQQSSVIAGVNVSIADFITLLILV
                     YLLFFANHLLKANHFLQFFIILYTYRMIITLCLLFFDDLIFITVKEVLASTVKYAFVV
                     IYFYLGMIIFKLGNSKKVIVTSYIISSVTIGLFCIIAGLNKSPLLMKLLYFDEIRSKG
                     LMNDPNYFAMTQIITLVLAYKYIHNYIFKVLACGILLWSLTTTGSKTAFIILIVLAIY
                     FFIKKLFSRNAVSVVSMSVIMLILLCFTFYNINYYLFQLSDLDALPSLDRMASIFEEG
                     FASLNDSGSERSVVWINAISVIKYTLGFGVGLVDYVHIGSQINGILLVAHNTYLQIFA
                     EWGILFGALFIIFMLYLLFELFRFNISGKNVTAIVVMLTMLIYFLTVSFNNSRYVAFI
                     LGIIVFIVQYEKMERDRNEE"
     gene            21369..22799
                     /locus_tag="O0293_000671"
     CDS             21369..22799
                     /locus_tag="O0293_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCY0279570.1"
                     /translation="MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVS
                     FSFNIVQYFLMIASVGVQLYFNRVIAKSVNDKRQLSQQFWDIFVSKLFLALTVFAMYM
                     VVITIFIDDYYLIFLLQGIYIIGAALDISWFYAGTEKFKIPSLSNIVASGIVLSVVVI
                     FVKDQSDLSLYVFTIAIVTVLNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPN
                     GQLNLYTSISCVVLGLVGTYQQVGIFSNAFNILTVAIIMINTFDLVMIPRITKMSIQQ
                     SHSLTKTLANNMNIQLILTIPMVFGLIAIMPSFYLWFFGEEFASTVPLMTILAILVLI
                     IPLNMLISRQYLLIVNKIRLYNASITIGAVINLVLCIILIYFYGIYGAAIARLITEFF
                     LLIWRFIDITKINVKLNIVSTIQCVIAAVMMFIVLGVVNHYLPPTMYATLLLIAIGIV
                     VYLLLMMTMKNQYVWQILRHLRHKTI"
BASE COUNT         7632 a         3555 c         4442 g         7269 t
ORIGIN      
        1 aataacaaca actatatgta taaaaaaacc tcgcaacggt tagttaactg aatcatttag
       61 ctaacttcgt tgtgaggtca ttttgtttta ataatatcgt tataactttt tcacggttaa
      121 taataagtat atgaagaatg gggcaccaaa agcagcaata aatacacctg ctggcacttc
      181 tttaggcaag aataaggtac gcccaattaa gtctgcaata acaattgata tggcaccaat
      241 cattgctgac attagtaact ttttagcata acttccgcga acgattgttt tcgcgatatg
      301 tggtgcgatt aaaccgacaa acccaatgtt acctactaaa ctgattgcca tagatacgag
      361 tatagtagaa gtgattaatt ggattagttt catacgttgt acatgtaagc ctaagccaat
      421 cgctacaggg tcatcaagta tagatatttt catttttggt ataacaagaa ataacaacgg
      481 cacaacagct aaaataacca tacccaaaat gattgtatct ttaaacgtag caccgtaaag
      541 acttccgact agccatgtat aagctttggc agcagataat tgcttcgttg taatgagtaa
      601 tccttggaca agcgcaataa acaacgtttg catcgaaata ccgatgatta tgagtgttgt
      661 cgggcgtatt tgtcctttcg tttgaaacac taatagtatc atcattgcaa ctgcgccacc
      721 taatactgca aatagtggaa gtaaatgtat tgttaaatgg ctgaaaaatg caataaagac
      781 aacagcactt aagctagcac cacctgtgat accgataata tcaggtgagg caattggatt
      841 ttttaataca ttttgcaaca ttaaaccact cattcctagt gcggcacctg ctaaaatcgc
      901 aagtgtaatg cgaggtaagc gtaatacttc taaagtgaat tgatccatac tgtcatttgg
      961 atttataaag tacatcagta cgcgttgtaa tggtataaag cttgaaccaa tcatcatact
     1021 taccactgaa acgatggcta aaaagattaa cgcgaagatg agatggtaat tgtctttttt
     1081 attaatcttt tcggtcataa gcgttgacgt cctttcttca taatatagat taagacaata
     1141 gcgccaatga cagcggtaac gacaccgata ggcaactcta gtggcttaat tattatacga
     1201 gcaacaatgt ctgaaatgat cattaggatt gctccagcta atgcagtaaa aggaattaaa
     1261 tacttatagt ttggtggtaa taatcgtttg ctaatattcg gtacgataag acccacaaag
     1321 acgattgatc cagctacggc taccgaaata ccggctaaca tactgatgag cataataatc
     1381 atccatttga ttaattttat gttttgaccg aggccggttg caatgtcgtc acttgtcatc
     1441 aagatgttga tgtgtgcagc catgctaaat gcaattaaaa taagtatcaa tacaagcgga
     1501 ataatccatg ggatatccca aatattacgt aatgaaacgg agccacttaa ccaaaataat
     1561 aggccttgta agtctgtttc gttcataata agtatgcctt gagtaaaggc tgtaaatagc
     1621 atcgcaatcg cagcacctgc caaaatgaca cggtgaggtg agaatagtgt ttgtctaaac
     1681 atacctagtg caacaactaa tacagtaaca acaatagccc ccaaaaatgc aataactaca
     1741 atcattttaa aagattgaat ttggataaat gtaatactaa aaatgacaaa aaatactgcg
     1801 cctgcattga caccgaaaag ccctggtgag gctattgggt ttcgtgtaag tgcttgcatc
     1861 aacaaacctg agacagcaag ggcagcacca gtcaataacg caatgattgt tctcgacgcc
     1921 cgtgcaccag tgacaacatc atgtaaatcg ttttcactat caaagttgaa taacgcctgt
     1981 atcaccgtac ctggtgacac aagcgtattt ccaatcatta aacttaagat agctactatt
     2041 gcaagacata aaccagcaat aacgatttgg tattttggtt taagtagcat cgtaaaactc
     2101 cttaattatt ttgattgttt ttcaatattt aacttttcat ataaatcgtc aataagtttt
     2161 aatgaagatt tatatccgcc agctaagttc caagtgattt catctaaatc atcagatact
     2221 tggttgtttt taactgcgtc taaatttttc cactctttac ttgaagtcca ttcgctttca
     2281 gtctttttaa ctaatgcagc atctttcgca tttggatctg attttactac aaaaatatga
     2341 tcagcgttca ttaatggaat gctttcttta gatgtaagtt ggataatatc tttaccatta
     2401 tcaacttgtt tttgtaagtc tttattacgt ttgaatccta aatcatttaa gatttcacca
     2461 gcatatccac cagcataaat tcttgtatga tcagcacgga agttaacaac tgaagctttc
     2521 aatggccatg catctttata ctttgctttt gcatcttttt ggaatgcagc tactttatca
     2581 tcgtactttt taagtaaatc ttcagcttct ttttctttcc ctaaagcttt ccccattaac
     2641 ttagttgtat ctttgaattt gaaaactgta tcagtagaaa ctgttggtgc gattttagat
     2701 aattgatcgt aaactttttc atttctaact tttgacgcga caattaagtc cggttttaat
     2761 ttagagattt cctctaagtt aggtgcaggt tcttgaccta caatcttagt atcttttaaa
     2821 tcatttttta tgtattcgaa tttcggtttt tgtgtccatg attctacagc acctacaggt
     2881 ttaacaccta aagatacagc gacgtcagtg gcaccttgat atagcgtaac aacacgcttt
     2941 ggtttccctt taatttcagt tgtacccatt gcatgtttaa ttgaagttgt ttccttatct
     3001 ttgttatcag atgattgttt atttgaattc ccactacatc ctgctaaaac aagtaggaaa
     3061 gcaagcgtaa caacaagcat tttaattact ttattcattg actaattagc ctccttcgtg
     3121 atgtatgaca atgagaatca ttatcacgat ttagtatgaa ttaaattttt tcctaagtca
     3181 ataaaatatt tatgatttac atgcaactta taattatttg acatataaat gcataaaaaa
     3241 tataatccta attacttgat agtgagaatc attatcaatt aggtaacaca caatattata
     3301 gaattttaaa tttgaggagg aagcgctttt gattgaaaaa agtcaagcat gtcacgattc
     3361 attgttagat tctgtagggc aaacacctat ggttcaactt catcaactat ttccgaaaca
     3421 tgaagtgttt gcaaagttag agtatatgaa tcctggaggc agcatgaaag atcgacctgc
     3481 caagtacatc attgaacatg gtattaaaca tggtttaatc actgagaata cacatttaat
     3541 tgaaagtact tctggtaatt taggcattgc gttggcaatg atagctaaaa tcaagggatt
     3601 aaaactcacg tgtgttgttg atcctaaaat atcaccaaca aatttgaaaa ttattaaaag
     3661 ttatggtgcc aatgtagaaa tggttgaaga acctgatgca catgggggtt atttaatgac
     3721 tcgtattgca aaggtgcaag aactgttagc cactattgac gatgcatatt ggattaatca
     3781 atatgcgaat gagttaaatt ggcaatccca ttatcatggt gcaggcacag agattgttga
     3841 aacaattaag caacctatag attattttgt cgcgccagtc agcacgacag gtagcattat
     3901 gggtatgagt agaaaaataa aagaagtgca tccaaacgca caaattgttg ctgttgatgc
     3961 gaaagggtca gtcatttttg gtgacaaacc tattaataga gaattacctg gtatcggtgc
     4021 tagtcgtgta cccgaaatat tgaatagatc agaaattaat caagtgatcc atgtagatga
     4081 ttatcaatct gctttgggct gtcgaaaact gattgattat gaaggcatat ttgccggagg
     4141 ttcaacaggt tcgattattg cagcgattga gcagttgata acgtcaattg aagaaggtgc
     4201 aacaattgtc acgattttac cagatcgagg cgatcgttac ttagatttag tttattcaga
     4261 tacatggtta gaaaaaatga aatcaagaca aggagttaaa tcagaatgaa tagagagatg
     4321 ttgtatttaa atagatcaga tattgaacaa gcgggaggta atcattcaca agtttatgtg
     4381 gacgcattaa cagaagcatt aacagcccat gcgcacaatg attttgtaca accgcttaag
     4441 ccgtatttaa gacaggatcc tgaaaatgga cacatcgcag atcgaattat tgcaatgcca
     4501 agtcatatcg gtggtgaaca cgcaatttca ggtattaagt ggataggtag taagcacgac
     4561 aatccatcga aacgtaatat ggagcgtgca agtggcgtca ttattttgaa tgatccagaa
     4621 acgaattatc caattgcagt tatggaagca agtttaatta gtagtatgcg tactgcagca
     4681 gtttcagtga ttgcagcaaa gcatttggct aaaaaaggat ttaaagactt aacaatcatt
     4741 ggatgcgggc taatcggaga caagcaatta caaagtatgt tagagcaatt cgatcatatt
     4801 gaacgcgtgt ttgtttacga tcaattctct gaagcatgtg cacgctttgt tgatagatgg
     4861 caacaacagc gtccggaaat taattttatt gcgacagaaa atgctaaaga agcagtatca
     4921 aatggtgaag tagtcattac atgtaccgta acggatcaac catacattga atatgattgg
     4981 ttacaaaagg gtgcatttat tagcaacatt tctatcatgg atgtgcataa agaagtcttt
     5041 attaaagctg acaaagtcgt agtagatgac tggtcacaat gtaatcgaga aaagaaaact
     5101 attaaccaat tggtgttaga aggtaaattc agcaaagaag ctcttcatgc tgaactagga
     5161 caacttgtga caggtgacat accaggacgt gaagacgatg atgagatcat attacttaat
     5221 ccgatgggta tggctatcga agatatttca agtgcttatt ttatttatca acaggcacaa
     5281 caacaaaata ttgggacaac attgaaccta tattaagaat gcgaggtgtc tgaacattgc
     5341 agaatcatac agcagtcaat acagcacaag cgataatatt aagagattta gttgatgcat
     5401 tattatttga agatatagcc ggaattgtat cgaatagtga gattactaaa gaaaacggac
     5461 aaacgctttt gatatacgaa cgtgaaacac aacaaataaa gatacctgtt tattttagtg
     5521 ctttaaatat gtttcgttac gaaagttcac aaccaattac gatagaggga agggtgtcta
     5581 agcaaccttt aacggcagct gaattttggc aaacaattgc taatatgaat tgtgatctaa
     5641 gtcatgaatg ggaagtggct cgcgttgaag aaggactgac tactgctgcc acacagcttg
     5701 ctaaacaatt atcagaatta gatttagcgt cacatccttt tgtgatgtca gagcagtttg
     5761 caagtttaaa agatcgtcca tttcatccat tagctaaaga aaaaagagga ttaagagaag
     5821 cggattatca agtgtatcaa gctgaattaa atcaatcatt tcctttaatg gttgcagcag
     5881 ttaaaaagac acatatgatt catggcgata ctgcaaatat cgatgaatta gaaaatttga
     5941 cagtacctat aaaagaacaa gcgacagaca tgttaaatga tcaagggtta tcaatagatg
     6001 actatgtact atttccggta catccttggc aatatcagca tattctgccg aacgtctttg
     6061 cgaaagagat tagtgaaaag ttggttgtac tattaccgtt aaaatttgga gattatctgt
     6121 cgtcttcaag tatgcgttca ttaattgata ttggcgcacc gtataaccat gtcaaagtac
     6181 catttgcaat gcagtcatta ggcgcattaa ggctaacgcc tacgcgttac atgaaaaacg
     6241 gagaacaagc agaacaatta ttacgtcagc ttatagaaaa agatgaagca ctagctaagt
     6301 atgtcatggt ttgtgatgaa acagcttggt ggtcatatat gggtcaagat aatgatattt
     6361 tcaaagatca attaggtcat ctaactgttc agctaagaaa gtatcccgaa gtgctagcca
     6421 aaaatgatac gcaacagcta gtgtcaatgg cagcactcgc ggcaaatgat cgcactttat
     6481 atcaaatgat ttgtggaaaa gataatattt ctaaaaatga tgtcatgacg ttatttgaag
     6541 atatcgcgca agtcttttta aaggtaacac tatcatttat gcaatacggc gcattaccag
     6601 agttgcatgg tcaaaatata ttgttgtcat ttgaagatgg acgtgtacaa aaatgcgtgt
     6661 tacgtgatca tgatactgtc agaatttata aaccatggct aacagcacat cagctttcat
     6721 tgccgaagta tgtcgtcaga gaagatacac ctaatacgct aattaatgag gatttggaaa
     6781 cattctttgc ttattttcaa acattagctg tatcggtaaa tctatatgcc attattgatg
     6841 caattcaaga tttatttggt gtaagtgagc atgaacttat gtcgttgtta aaacaaattt
     6901 taaaaaatga agtggcaact atttcctggg ttacaactga tcagctagct gtcagacaca
     6961 ttttatttga taaacagacg tggccattca aacaaatttt attaccattg ctatatcaac
     7021 gtgatagtgg tggaggtagt atgccttcag gtttaactac cgtaccaaat ccaatggtga
     7081 catatgatta atcagtctat atggcgcagt aactttcgca ttttatggct cagtcagttt
     7141 atagcgattg ctggactgac agtacttgtg ccattattgc caatttatat ggcatcacta
     7201 caaaatctat cagtcgtaga aatacagttg tggagtggta tagcgattgc tgctccagct
     7261 gtaacgacga tgatagcttc gccgatatgg gggaagctag gtgataagat cagccgaaaa
     7321 tggatggtgt taagagcgtt acttggtttg gcggtatgct tatttttaat ggcattgtgt
     7381 acgacaccat tacagtttgt acttgtgagg ttattgcagg gactatttgg tggtgttgtt
     7441 gatgcatcaa gtgcgtttgc gagtgcagag gcgccagctg aagatcgtgg aaaggtatta
     7501 ggaagactgc aaagttcagt cagcgcaggg tctcttgtgg ggccattaat tggcggtgtt
     7561 acagcttcga tattaggttt tagtgcgtta ctgatgagta ttgccgttat tacttttatt
     7621 gtctgtattt tcggtgcatt aaaattgatt gaaacgacac atatgccaaa atcacaaaca
     7681 ccaaatatta ataaaggtat tcgccgttca tttcaatgtc tattatgcac acaacaaaca
     7741 tgtcgattta ttatcgttgg cgttttagca aactttgcta tgtatggcat gctaactgca
     7801 ttatcaccac ttgcttcatc agtgaatcat acagcgatag atgaccgtag tgtgattgga
     7861 tttttacagt ccgcattttg gacggcttcg atattaagcg cgcctttatg gggacgcttt
     7921 aatgataaat catatgttaa atcagtatat atatttgcca cgattgcatg tggttgtagt
     7981 gcgatactgc aaggtttagc gacgaatata gagtttttaa tggctgcaag aatacttcaa
     8041 ggattaacat atagtgcatt gattcaaagt gtcatgtttg ttgtcgtgaa tgcgtgtcat
     8101 caacaactta aaggcacatt tgttggaacg acgaacagta tgttagttgt tggtcaaatt
     8161 attggcagtc ttagtggcgc tgccattaca agttatacta caccagctac tacgtttatc
     8221 gttatgggcg tagtatttgc agtaagtagt ttatttttaa tttgttcaac catcactaat
     8281 caaatcaacg atcacacatt aatgaaatta tgggagttga aacaaaaaag tgcaaaataa
     8341 agaattaata caacatgcag cgtatgcggc tatcgaacgc attttaaatg aatattttag
     8401 agaagaaaat ttatatcaag taccacctca aaatcatcaa tggtctatac aattatcaga
     8461 gctcgaaact ttaacgggtg aatttcgcta ttggtctgcg atggggcatc atatgtatca
     8521 tccagaggta tggcttatcg atggaaaaag taaaaaaata acaacttata aagaagcaat
     8581 tgcgcgtatt ttgcaacata tggctcaaag tgcagataat caaacggcag tgcaacaaca
     8641 tatggcgcaa attatgtctg acatcgataa tagcattcat cgcacggcac gttatttgca
     8701 aagtaacaca atagactacg tagaggatcg ttatatcgtt tcagaacaat ctttatactt
     8761 aggtcatcca tttcatccga ctcctaagag tgcaagtggg ttttcagaag cagatttaga
     8821 gaaatatgca cccgaatgtc atacatcatt ccaattgcat tatttagctg tgcatcaaga
     8881 tgttctgctc acgcgctatg tagaaggtaa agaagatcag gttgagaaag tgttgtatca
     8941 attagcagac atagatatat cagagatacc caaagatttt attttattac caacacatcc
     9001 ttatcaaatc aatgtgttgc gacagcatcc acagtatatg caatatagtg aacaaggttt
     9061 aataaaagac cttggcgttt ccggtgattc agtgtacccg acgtcttcgg ttagaactgt
     9121 attttcaaaa gcattaaaca tttatttaaa attaccgata cacgttaaaa tcactaattt
     9181 tatacgtacg aatgaccttg aacagattga acggacaatt gatgccgcgc aagttatcgc
     9241 atcagtcaaa gatgaggttg aaacacccca ttttaaattg atgtttgaag aaggatatcg
     9301 tgcattgtta ccgaatccat tagggcaaac agttgaacct gaaatggatt tattaacaaa
     9361 tagtgccatg attgttcgtg aagggatacc gaattaccat gctgataaag atattcatgt
     9421 attggcgtca ttatttgaaa cgatgcctga ttcaccgatg tctaagttat cacaagtgat
     9481 tgagcaaagt ggtttagcac cagaagcatg gcttgaatgt tatttgaatc gtacattatt
     9541 gccgatatta aagctgttta gtaacacagg cattagtcta gaagcacatg tacaaaatac
     9601 attaattgaa ttaaaagatg gcatacccga cgtatgcttt gtcagagatc ttgaaggcat
     9661 ttgtctatct agaacgattg ctactgaaaa acagcttgtg ccaaatgttg tggcagcatc
     9721 aagccctgtt gtatatgcac atgatgaagc atggcatcgt cttaaatatt acgttgtagt
     9781 aaatcactta ggacatttag tatcaactat tggtaaagcg actagaaatg aagttgtgtt
     9841 atggcaactt gtagcgcatc gtcttatgac ttggaaaaaa gaatacgcga ataacgcagt
     9901 atttgttgac tgtgtagaag atttatatca aacgccgacc attgcggcta aagcgaattt
     9961 gatgagtaaa ttgaatgatt gtggtgcaaa ccctatttat acacatatac caaatccaat
    10021 ttgtcataac aaggaggtat cgtattgtga atcaaacaat tcttaatcgt gtaaagacta
    10081 gagtgatgca ccaactggta tcatcactta tttatgagaa tattgttgtg tataaagcgt
    10141 catatcaaga cggtgtcggt cattttacaa tagaaggaca tgattcagag tatcgtttta
    10201 ctgctgaaaa gacacatagc tttgatcgta tacgtatcac atcaccaatt gagcgtgtcg
    10261 taggagatga ggcagataca acaacagact atacacaatt attgagagag gttgtattta
    10321 catttcctaa aaatgatgaa aagctagaac aatttattgt cgagttatta cagacagaat
    10381 taaaagatac gcaaagtatg cagtatcgag aatcaaaccc accagcaaca cctgagacat
    10441 ttaacgacta tgaattttat gcgatggaag ggcatcagta tcatccaagt tacaaatcac
    10501 gtttaggatt tacgttgagt gataatttga aatttggtcc tgattttgta ccaaacgtta
    10561 aactgcagtg gttagctatc gacaaagata aagtagaaac gacggtatca agaaatgttg
    10621 tagttaacga aatgttacgt caacaagttg gcgataagac ttatgaacat tttgtacagc
    10681 agattgaagc atctggcaaa catgtaaatg atgttgagat gatacctgta cacccatggc
    10741 agtttgaaca tgtcatccaa gttgatttgg ctgaagaaag gcttaatggc acagtactat
    10801 ggttagggga aagtgatgag ctatatcacc ctcaacaatc gattcgtacg atgtcgccaa
    10861 tagacacgac aaaatattat ttaaaggtac caataagtat aacgaacact tcaacgaaac
    10921 gagtgttggc gcctcataca attgaaaatg cagcgcaaat tacggattgg ttaaagcaga
    10981 tacagcaaca agatatgtat ttaaaagatg aattaaagac agtttttcta ggggaagtct
    11041 taggacagtc ttatttaaat acacaacttt cgccttataa acaaactcaa gtttatggtg
    11101 cgttaggtgt tatatggcgt gaaaatatat atcatatgtt aatcgatgaa gaggatgcga
    11161 taccatttaa tgcactttat gcaagtgata aggatggtgt accattcatt gaaaattgga
    11221 ttaaacaata tggttctgaa gcttggacaa agcaattttt agctgtagcg attcgtccaa
    11281 tgattcatat gctttattat cacggtattg cctttgaatc gcatgcacaa aatatgatgc
    11341 tcattcatga aaatggttgg cctacacgta ttgccttaaa agatttccat gatggtgttc
    11401 gttttaagcg tgagcattta agtgaagctg cttcacacct gacattaaag ccaatgccag
    11461 aagcacataa aaaagtgaat agtaattcat ttattgaaac agatgacgaa cgtttagtac
    11521 gcgacttttt acatgatgca tttttcttta ttaatatcgc cgaaatcatc ttatttattg
    11581 aaaagcaata tggtatcgat gaggagctgc aatggcaatg ggttaaaggc atcatcgagg
    11641 cgtatcaaga agcatttcca gagttgaata actatcaaca tttcgatttg tttgaaccta
    11701 cgattcaagt tgaaaagtta acgacacgtc gattattaag tgactccgag ttaagaattc
    11761 atcatgttac aaatccatta ggtgtaggag gtatcaatga tgcaacaact atctctgaaa
    11821 catagattaa acaatggtga ttcagtttat ggcattttta attctatacc ggacccattg
    11881 atgatcgagg ttatcgcagc aagcgggtat gactttgttg tgattgatac agaacacgtg
    11941 gcgattaatg atgagacact agcgcattta attcgtgcag ctgaagcagc gcatattata
    12001 ccaattgtac gtgtcactgc agtgatagat agagatatca ttaaagtgtt ggatatgggt
    12061 gcgagaggta ttattgtgcc acacgttaaa gatcgtgaga cagttgagca tattgtgaaa
    12121 ttaagtcgtt attacccgca aggattaaga agtttgaatg gtggtcgcat ggcaagattt
    12181 ggacgtacac cattacttga tgcaatggag atggctaatg agcatattat ggtgattgcc
    12241 atgatagaag atgttgaagg ggttatggcc attgacgata tagcacaagt cgaaggttta
    12301 gacatgatag tcgaaggtgc cgcagattta tcgcagtcac ttggcatacc atggcaaacg
    12361 cgtgatgatc aagtaacatc acatgttcaa catatttttg aagttgtgaa tgcacatggt
    12421 aaacattttt gtgcattacc acgtgaagat gaagatattg caaaatggca ggcacaaggt
    12481 gtacaaacat ttattttagg tgatgatcgc ggaaaaatat atcgccattt aagtgcatct
    12541 ctagcgacgt ctaaacagaa aggggatgaa ggctaatgcg tatagttcaa cctgttattg
    12601 aacaattaaa agcacaatct catccagttt gtcattatat ctatgattta gtcggactgg
    12661 aacatcattt gcaacatatt acatcgtcat tgccgagtaa ttgtcaaatg tactatgcaa
    12721 tgaaagcaaa tagtgaacga aaaatcctag atacaattag tcagtatgtt gaaggattcg
    12781 aagttgcatc tcaaggtgaa atagcaaaag gtcttgcttt taaaccagca aatcatatta
    12841 tttttggtgg ccctggtaag acagacgagg aactaagata tgcagtaagt gaaggtgttc
    12901 agcgtattca tgttgaaagt atgcatgaat tacaacggct aaatgccatc ttagaagatg
    12961 aagataagac acaacacatt ttattgcgtg ttaatttagc aggaccattt cccaatgcaa
    13021 cgttgcatat ggcaggacgc ccaacacaat ttggtatttc tgaagacgaa gttgatgatg
    13081 tcattgaagc tgcgctcgca atgccaaaga ttcatctaga tggatttcat tttcattcta
    13141 tttctaacaa tttagactcg aatttacatg tcgatgtagt gaaactttat tttaaaaaag
    13201 caaaggcatg gtctgaaaaa catcgatttc cactcaaaca tatcaatctt ggtggtggca
    13261 taggcgttaa ctatgcagat ttaactaacc aatttgaatg ggataatttt gtagaacgtt
    13321 ttaaaacact tatcgttgag caagaaatgg aagatgtgac attgaacttt gaatgtgggc
    13381 gctttattgt ggcacatatt ggttactatg tgacagaagt gctagacatt aagaaagtac
    13441 atggtgcttg gtatgccata ttaagaggag gtacgcaaca atttagactg ccggtatctt
    13501 ggcaacataa ccatcctttt gacatttatc gctataagga caatccatat tcatttgaaa
    13561 aagtttcaat ttcgagacag gacacaacgt tagtcggtca attatgtaca ccgaaagatg
    13621 tctttgctag agaagtacag atagacgcaa tcagtacagg cgacgttatt gttttcaaat
    13681 atgcaggtgc atacggatgg tctatttcac atcacgattt cttaagccat ccacatcctg
    13741 aatttattta tttaacgcaa acaaaggagg atgaataact attgaatcat attcatgaac
    13801 atttaaaatt ggtaccagta gataagattg atcttcacga aacattcgaa cctttaagat
    13861 tggaaaaaac gaaaagtagt attgaagcag atgattttat acgtcatcct attttagtga
    13921 cagcgatgca acatggtaga tatatggtta tagatggtgt gcatcggtat acaagtttga
    13981 aagcgttagg atgtaagaaa gttccagtgc aagaaatcca tgaaacacaa tattcaatta
    14041 gtacatggca acataaagtt ccatttggtg tgtggtggga aacgttacaa caagaacatc
    14101 gcttgccatg gactactgag acaagacaag aagcgccatt tattacaatg tgtcatggtg
    14161 atacagaaca atatttgtat acaaaagatt taggcgaagc acattttcaa gtatgggaaa
    14221 aggttgtcgc aagttatagt ggttgttgtt ctgtagagag aattgcacaa ggtacatatc
    14281 cttgtctttc tcaacaagat gtactcatga agtatcagcc attgagttat aaggaaattg
    14341 aagcggttgt tcataaaggg gaaactgtgc cagcaggtgt gacacgcttt aatatttcag
    14401 gacgatgtct taatcttcaa gtaccactgg cattacttaa acaagatgat gatgttgaac
    14461 aactgcgcaa ttggaagcag tttttagcag ataagtttgc caatatgaga tgctatactg
    14521 aaaaagtata cttggtggag caatagtttt actgtgatgt tgagggaaat atgatgattt
    14581 agcgtattga tagcgaaaat ataataaaac aatatagtgt ggagaacttt tgatatttta
    14641 taaatattga agttctccat ttttgtattt tgcatataaa aattaaataa aataaggtat
    14701 attaaggtaa agtataaatt ttaaataaat ggggaatgag tatgagctca attataggaa
    14761 aaatagcaat ttggataggc atcgtagctc aaatatattt tagtgtcgtt tttgttagga
    14821 tgatatctat taatattgct ggaggatctg attacgaaac aattttttta ttaggattaa
    14881 tattggctct tttcactgtt ttaccaacca tctttactgc gatttatatg gaaagttact
    14941 ctgtaatcgg aggtgcactt tttattgttt atgctattat tgcactgtgt ttatataatt
    15001 tcctttcgtc aattttatgg ctgattggtg gtattttgct gatttggaat aaatactcaa
    15061 aagatgaatc gacagacgaa aatgaaaaag ttgatattga aagtacagag aatcaatttg
    15121 aatctaaaga taaaatcact aaagaataaa gagaatattt aaggtaaagt ataaatttta
    15181 aataaatggg gaatagacat ggaaaaaaat gtagaaaaat cattcataaa gataggttta
    15241 tattttcaaa tagcttatat agtactcatg gctataactt tatgtgggtt tgtaatttgc
    15301 tatggactaa ttttcggcct tttctattta ttatcaggta gcagagctga ttatttaata
    15361 gtaacaatag ttatatcggc aataatttct atatttgtaa ttatactttc aatcgtacct
    15421 gtcatcgtat tggcatctga cttatttaaa gaaaggattt caaaaggtgt catattaatt
    15481 gtattggcta ttatcgcttt agtattatgc aactttgtat ctgcaatact ctggtttgtt
    15541 tcagccatat ctattttagg tagaaaaaaa ttagtagctg cagcagatac taccactatt
    15601 caaaaaagta aagggaacgc aaatcaagca tcacataaag acacgtgtaa aaaggaactt
    15661 gatagtcaag acatgatgga acatcctgag gttaaaaatc ccacgactaa aaaccttgaa
    15721 ggatttaacg aagaaataca taaagatgaa gctacaacta aagttgtcag tgataacacg
    15781 gaaccgccta ttgaatcaaa agaccatgtc tcgaaaaaag attgatgaca aactaatcga
    15841 gagacttaaa aaaataatat tcaacataag aacttttaaa acgacattta aacgcattgc
    15901 caatcactaa tggtagtgcg tttaactata ccttaaatat ctgaatattt tgttaaatgg
    15961 agctaccttt gttgtactat tcaaatgaag aggagtaaaa tgtaattaaa ggaaagaaat
    16021 ttgaggagtg atctttatga caaacaacaa agtagcatta gtaactggcg gagcacaagg
    16081 gattggtttt aaaattgcag aacgtttagt ggaagatggt ttcaaagtag cagttgttga
    16141 tttcaatgaa gaaggggcaa aagcagctgc acttaaatta tcaagtgatg gtacaaaagc
    16201 tattgctatc aaagcagatg tatcaaaccg tgatgatgta tttaacgcag taagacaaac
    16261 tgccgcgcaa tttggcgatt tccatgtcat ggttaacaat gccggccttg gaccaacaac
    16321 accaatcgat acaattactg aagaacagtt taaaacagta tatggcgtga acgttgcagg
    16381 tgtgctatgg ggtattcaag ccgcacatga acaatttaaa aaattcaatc atggcggtaa
    16441 aattatcaat gcaacatctc aagcaggcgt tgagggtaac ccaggcttgt ctttatattg
    16501 cagtacaaaa ttcgcagtgc gaggtttaac acaagtagcc gcacaagatt tagcgtctga
    16561 aggtattact gtgaatgcat tcgcacctgg tatcgttcaa acaccaatga tggaaagtat
    16621 cgcagtggca acagccgaag aagcaggtaa acctgaagca tggggttggg aacaatttac
    16681 aagtcagatt gctttgggca gagtttctca accagaagat gtttcaaatg tagtgagctt
    16741 cttagctggt aaagactctg attacattac tggacaaaca attattgtag atggtggtat
    16801 gagattccgt taataatcat ccactaatga taaataaatc cttattgtta agtttaatca
    16861 cttagcagta aggatttttt agtgcactta gaagggagtg tattggtaga aaattaataa
    16921 gcgaagttct taagtgagtt atgatgtcac agtctaatgc atcagttgaa agcattatta
    16981 gtattaacac acccaagata ttataaaaca tcacaaaaac accactatct aatttatctc
    17041 aataaaaatt cacaaagtta tctcatttta tttttataaa taaaaaatat cgataaaaag
    17101 cttacaatac tttatgtttt tatgatatat ttttaatgta taaatgaggt ggaagatttg
    17161 gaaagagttt tgataactgg tggggctggt tttattgggt cgcatttagt agatgattta
    17221 caacaagatt atgatgttta tgttctagat aactatagaa caggtaaacg agaaaatatt
    17281 aaaagtttgg ctgacgatca tgtgtttgaa ttagatattc gtgaatatga tgcagttgaa
    17341 caaatcatga agacatatca atttgattat gttattcatt tagcagcatt agttagtgtt
    17401 gctgagtcgg ttgagaaacc tatcttatct caagaaataa acgtcgtagc aacattaaga
    17461 ttgttagaaa tcattaaaaa atataataat catataaaac gttttatctt tgcttcgtca
    17521 gcagctgttt atggtgatct tcctgatttg cctaaaagtg atcaatcatt aatcttacca
    17581 ttatcaccat atgcaataga taaatattac ggcgaacgga cgacattaaa ttattgttcg
    17641 ttatataaca taccaacagc ggttgttaaa ttttttaatg tatttgggcc aagacaggat
    17701 cctaagtcac aatattcagg tgtgatttca aagatgttcg attcatttga gcataacaag
    17761 ccatttacat tttttggtga cggactgcaa actagagatt ttgtatatgt atatgatgtt
    17821 gttcaatctg tacgcttaat tatggaacac aaagatgcaa ttggacacgg ttataacatt
    17881 ggtacaggca cttttactaa tttattagag gtttatcgta ttattggtga attatatgga
    17941 aaatcagtcg agcatgaatt taaagaagca cgaaaaggag atattaagca ttcttatgca
    18001 gatatttcta acttaaaggc attaggattt gttcctaaat atacagtaga aacaggttta
    18061 aaggattact ttaattttga ggtagataat attgaagaag ttacagctaa agaagtggaa
    18121 atgtcgtgaa aatgacattg aagctgtcca taataataag ggttatgcct atcaaagaaa
    18181 attagacaaa ctagaagaag tgagaaaaag ctattaccca attaaacgtg cgattgactt
    18241 aattttaagc attgttttat tatttttaac tttaccgatt atggttatat tcgccattgc
    18301 tatcgtcata gattcgccag gaaaccctat ttatagtcag gttagagttg ggaagatggg
    18361 taaattaatt aaaatataca aattacgttc gatgtgcaaa aacgcagaga aaaacggtgc
    18421 gcaatgggct gataaagatg atgatcgtat aacaaatgtc gggaagttta ttcgtaaaac
    18481 acgcattgat gaattaccac aactaattaa tgttgttaaa ggggaaatga gttttattgg
    18541 accacgcccg gaacgtccgg aatttgtaga attatttagt tcagaagtga taggtttcga
    18601 gcaaagatgt cttgttacac cagggttaac aggacttgcg caaattcaag gtggatatga
    18661 cttaacaccg caacaaaaac tgaaatatga catgaaatat atacataaag gtagtttaat
    18721 gatggaacta tatatatcaa ttagaacatt gatggttgtt attacagggg aaggctcaag
    18781 gtagtcttaa tttacttaat aagttcaaat aaaagttata ttttaaagat tgtgaccaat
    18841 tgttacagta taacgaggaa tcccttgaga cagtatcaaa tggcattaag aaatatgtgc
    18901 catcattgat ttgcatggct ataaatacta ttcatctgat gagatagcca tgttaagaaa
    18961 ttgaaagtat agcattaaag gggtttgtaa cagttgaaaa ttatatattg tattactaaa
    19021 gcagacaatg gtggtgcaca aacacatctc attcaactcg ccaaccattt ttgcgtacac
    19081 aatgatgttt atgtcattgt aggcaatcat ggaccaatga ttgaacaact agatgcaaga
    19141 gttaatgtaa ttattatcga acatttagta ggtccaattg actttaaaca agatatttta
    19201 gctgtcaaag tgttagcaca gttattctcg aaaattaaac ctgatgttat ccatttacat
    19261 tcttccaaag ctggaacggt cggacgaatt gcgaagttca tttcgaaatc gaaagacaca
    19321 cgtatagttt ttactgcaca tggatgggct tttacagagg gtgttaaacc agctaaaaaa
    19381 tttctatatt tagttatcga aaaattaatg tcacttatta cagatagcat tatttgtgtt
    19441 tcagatttcg ataaacagtt agcgttaaaa tatcgattta atcgattgaa attaaccaca
    19501 atacataatg gtattgcaga tgttcccgct gttaagcaaa cgctaaaaag ccaatcacat
    19561 aacaatattg gcgaagtagt tggaatgttg cctaataaac aagatttaca gattaatgcc
    19621 ccgacaaagc atcaatttgt tatgattgca agatttgctt atccaaaatt gccacaaaat
    19681 ctaatcgcgg caatagagat attgaaatta cataacagta atcatgcgca ttttacattt
    19741 ataggcgatg gacctacatt aaatgattgt cagcaacaag ttgtacaagc tgggttagaa
    19801 aatgaagtca catttttggg caatgtcatt aatgcgagtc atttattatc acaatacgat
    19861 acgtttattt taataagtaa gcatgaaggt ttgccaatta gcattataga agctatggct
    19921 acaggtttgc ctgttatagc cagtcatgtt ggcggtattt cagaattagt agctgataat
    19981 ggtatatgta tgatgaacaa ccaacccgaa actattgcta aagtcctgga aaaatattta
    20041 atagacagtg attacatcaa aatgagtaat caatctagaa aacgttattt agaatgtttt
    20101 actgaggaga aaatgattaa agaagtggaa gacgtttata atggaaaatc aacacaatag
    20161 taaattacta acattgttac ttatcggttt agcggttttt attcagcaat cttcggttat
    20221 tgccggtgtg aatgtttcta tagctgactt tatcacatta ctaatattag tttatttact
    20281 gtttttcgct aaccatttat taaaggcaaa tcatttttta cagtttttca ttattttgta
    20341 tacatatcgt atgattatta cgctttgttt gctatttttt gatgatttga tatttattac
    20401 ggttaaggaa gttcttgcat ctacagttaa atatgcattt gtagtcattt atttctattt
    20461 agggatgatc atctttaagt taggtaatag caaaaaagtg atcgttacct cttatattat
    20521 aagcagtgtg actataggtc tattttgtat tatagctggt ttgaacaagt cccctttact
    20581 aatgaaattg ttatattttg atgaaatacg ttcaaaagga ttaatgaatg accctaacta
    20641 tttcgcgatg acacagatta ttacattggt acttgcttac aagtatattc ataattacat
    20701 attcaaggtc cttgcatgtg gtattttgct atggtcttta actacaacgg ggtctaagac
    20761 tgcgtttatc atattaatcg tcttagccat ttatttcttt attaaaaagt tatttagtag
    20821 aaatgcggta agtgttgtga gtatgtcagt gattatgctg atattacttt gttttacctt
    20881 ttataatatc aactactatt tattccaatt aagcgacctt gatgccttac cgtcattaga
    20941 tcgaatggcg tctatttttg aagagggctt tgcatcatta aatgatagtg ggtctgagcg
    21001 aagtgttgta tggataaatg ccatttcagt aattaaatat acactaggtt ttggtgtcgg
    21061 attagtggat tatgtacata ttggctcgca aattaatggt attttacttg ttgcccataa
    21121 tacatatttg cagatctttg cggaatgggg cattttattc ggtgcattat ttatcatatt
    21181 tatgctttat ttactgtttg aattatttag atttaacatt tctgggaaaa atgtaacagc
    21241 aattgttgta atgttgacga tgctgattta ctttttaaca gtatcattta ataactcaag
    21301 atatgtcgct tttattttag gaattatcgt ctttattgtt caatatgaaa agatggaaag
    21361 ggatcgtaat gaagagtgat tcactaaaag aaaatattat ttatcaaggg ctataccaat
    21421 tgattagaac gatgacacca ctgattacaa tacccattat ttcacgtgca tttggtccca
    21481 gtggtgtggg tattgtttca ttttctttca atatcgtgca atactttttg atgattgcaa
    21541 gtgttggcgt tcagttatat tttaatagag ttatcgcgaa gtccgttaac gacaaacggc
    21601 aattgtcaca gcagttttgg gatatctttg tcagtaaatt atttttagcg ttaacagttt
    21661 ttgcgatgta tatggtcgta attactatat ttattgatga ttactatctt attttcctac
    21721 tacaaggaat ctatattata ggtgcagcac tcgatatttc atggttttat gctggaactg
    21781 aaaagtttaa aattcctagc ctcagtaata ttgttgcgtc tggtattgta ttaagtgtag
    21841 ttgttatttt tgtcaaagat caatcagatt tatcattgta tgtatttact attgctattg
    21901 tgacggtatt aaaccaatta cctttgttta tctatttaaa acgatacatt agctttgttt
    21961 cggttaattg gatacacgtc tggcaattgt ttcgttcgtc attagcatac ttattaccaa
    22021 atggacagct caacttatat actagtattt cttgcgttgt tcttggttta gtaggtacat
    22081 accaacaagt tggtatcttt tctaacgcat ttaatatttt aacggtcgca atcataatga
    22141 ttaatacatt tgatcttgta atgattccgc gtattaccaa aatgtctatc cagcaatcac
    22201 atagtttaac taaaacgtta gctaataata tgaatattca attgatatta acaataccta
    22261 tggtctttgg tttaattgca attatgccat cattttattt atggttcttt ggtgaggaat
    22321 tcgcatcaac tgtcccattg atgaccattt tagcgatact tgtattaatc attcctttaa
    22381 atatgttgat aagcaggcaa tatttattaa tagtgaataa aataagatta tataatgcgt
    22441 caattactat tggtgcagtg ataaacctag tattatgtat tattttgata tatttttatg
    22501 gaatttacgg tgctgctatt gcgcgtttaa ttacagagtt tttcttgctc atttggcgat
    22561 ttattgatat tactaaaatc aatgtgaagt tgaatattgt aagtacgatt caatgtgtca
    22621 ttgctgctgt tatgatgttt attgtgcttg gtgtggtcaa tcattatttg ccccctacaa
    22681 tgtacgctac gctgctatta attgcgattg gtatagtagt ttatctttta ttaatgatga
    22741 ctatgaaaaa tcaatacgta tggcaaatat tgaggcatct tcgacataaa acaatttaag
    22801 taccggtaat gctatacttt agaaaattaa gattaagaag aaaaggcaat ttcttattga
    22861 aaaatggaag ttgtcttttt taattctctt taaaagcg
//
LOCUS       DAKTIO010000022        22836 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.022, whole genome shotgun sequence.
ACCESSION   DAKTIO010000022 DAKTIO010000000
VERSION     DAKTIO010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22836
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.022"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..849)
                     /locus_tag="O0293_000672"
     CDS             complement(<1..849)
                     /locus_tag="O0293_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HCY0279571.1"
                     /translation="MSVNARDLLVLHTNVNRLVGEEIFANKCLANNDVQIMNSIKKLI
                     EAELLTTTNDFEVSIYKKTRPELQSILKSFGIKTTGNKPDLIKRIDDNFHIINNLDLP
                     YVYIPTKKGEEILKKTEYLTSFIQSYGEISLERAYYLVENYIDENCDDKVAEIYKFEF
                     QRKYDNGEFDFNHGYNFELNMLIDHYKRDVKDYDNARKYSNIYLYFGLRDFLKKLMSN
                     YSYYDSKGNIDLNEIQNDLNRFINSSASGMYERLIYNENLSNNIMFELFKKDTQDYSD
                     LEEQLIE"
     gene            1138..1230
                     /locus_tag="O0293_000673"
     CDS             1138..1230
                     /locus_tag="O0293_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279572.1"
                     /translation="MFIFKTTSKSHFHNNVKSLECIKIPINKNR"
     gene            complement(1935..2792)
                     /locus_tag="O0293_000674"
     CDS             complement(1935..2792)
                     /locus_tag="O0293_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCY0279573.1"
                     /translation="MDNVKAIFLDMDGTILHENNQASTYTKDVINQLREKGYKVFLAT
                     GRSHSEIHQLVPQDFAVNGIISSNGTIGEVDGEIIFKHGLSLAQVQQITNLAKRQQIY
                     YEVFPFEGNRVSLKEDETWMRDMIRSQDPINGVSHSEWSSRQDALAGKIDWVTKFPEG
                     EYSKIYLFSSNLEKITAFRDELKQNHVQLQISVSNSSRFNAETMAYQTDKGTGIKEMI
                     AHFGIHQEETLVIGDSDNDRAMFEFGHYTVAMKNARPEIQALTSDVTAYTNEEDGAAK
                     YLAEHFLAE"
     gene            complement(2860..3642)
                     /locus_tag="O0293_000675"
     CDS             complement(2860..3642)
                     /locus_tag="O0293_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0279574.1"
                     /translation="MLIQLDQIGRMKQGKTILKKISWQIAKGDKWILYGLNGAGKTTL
                     LNILNAYEPATSGTVNLFGKMPGKVGYSAETVRQHIGFVSHSLLEKFQEGERVIDVVI
                     SGAFKSIGVYQDIDDEIRNEAHQLLKLVGMFAKAQQYIGYLSTGEKQRVMIARALMGQ
                     PQVLILDEPAAGLDFIARESLLSILDSLSDSYPTLAMIYVTHFIEEITANFSKILLLK
                     DGQSIQQGAVEDILTSENMSRFFQKNVAVQRWNNRFSMAMLE"
     gene            complement(3874..5679)
                     /gene="glmS"
                     /locus_tag="O0293_000676"
     CDS             complement(3874..5679)
                     /gene="glmS"
                     /locus_tag="O0293_000676"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCY0279575.1"
                     /translation="MCGIVGYIGYDNAKELLLKGLEKLEYRGYDSAGIAVVNDDNTTV
                     FKEKGRIAELRKVADSSDFDGPVGIGHTRWATHGVPNHENSHPHQSSNGRFTLVHNGV
                     IENYEELKGEYLQGVSFISETDTEVIVQLVEYFSNQGLSTEEAFTKVVSLLHGSYALG
                     LLDAEDKDTIYVAKNKSPLLLGVGEGFNVIASDALAMLQVTSEYKEIHDHEIVIVKKD
                     EVIIKDADGNVVERDSYIAEIDASDAEKGVYAHYMLKEIHEQPAVMRRIIQEYQDAEG
                     NLKIDQDIINDVKEADRIYVIAAGTSYHAGLVGKEFLEKWAGVPTEVHVASEFVYNMP
                     LLSEKPLFVYISQSGETADSRAVLVETNKLGHKSLTITNVAGSTLSREADHTLLLHAG
                     PEIAVASTKAYTAQIAVLSILSQIVAKEHGREADIDLLRELAKVTTAIEAIVDDAPIM
                     EQIATDFLETTRNAFFIGRTIDYNVSLEGALKLKEISYIQAEGFAGGELKHGTIALIE
                     EGTPVVGLATQEKVNLSIRGNVKEVVARGAHPCIISMEGLEKEGDTYVIPHVHELLTP
                     LVSVVALQLISYYAALHRDLDVDKPRNLAKSVTVE"
     gene            6360..7898
                     /locus_tag="O0293_000677"
     CDS             6360..7898
                     /locus_tag="O0293_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCY0279576.1"
                     /translation="MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFID
                     NGWLPNKDLATLAGPMITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPM
                     LLGAMIMGPLVGWLMKKTDQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLM
                     KFIMHILSVAVEALVHAHLLPLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQS
                     ILYTIESNPGPGLGILLAYMIFGKGTAKATSYGAGIIHFLGGIHEIYFPYVLMRPLLF
                     IAVILGGMTGVATYQATGFGFKSPASPGSFIVYCLNAPRGEFLHMLLGVFLAALVSFV
                     VAALIMKFTREPKQDLEAATAQMENTKGKKSSVASKLVSSDKNVNTEENASGNVSETS
                     SSDDDPEALLDNYNTEDVDAHNYNNINHVIFACDAGMGSSAMGASMLRNKFKKAGIND
                     ITVTNTAINQLPKDAQLVITQKKLTDRAIKQTPNAIHISVDNFLNSPRYEELLNNLKK
                     DDQA"
     gene            7933..10065
                     /locus_tag="O0293_000678"
     CDS             7933..10065
                     /locus_tag="O0293_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCY0279577.1"
                     /translation="MLLSTREKEMIALLIKYHGQYITIHDIAQQLAVSSRTIHRELKG
                     VEAYLTSFSLTLERANKKGLRIAGTDSDLNDLKQSIAQHQTIDLSVEEQKVIIIYALI
                     QAKEPVKQYSLAQEIGVSVQTLAKMLDDLELDLNKYQLSLSRKRGEGIYLVGTESKKR
                     EFLSQLMVNNLNSTSVYSVIENHFVFHSLNQIHKDFVDLERIFNVERLLMDYLSALPY
                     QLTESSYLTLTVHIVLSISRIKNGEYVALNDDIYDSVQNTFEHKVASELADKLGQIYD
                     VTFNQAEIAFITIHLRGAKRKNLNDTSLNNRCEENKIKAFVNKVEMISGMTFADLDTL
                     VDGLTLHLNPAINRLQANIETYNPLTDMIKFKYPRLFENVRLALNDCWPDLIFPESEI
                     AFIVLHFGGSIKNQGNRFLNILVVCSSGMGTSRLLSTRLDQVFSEIERITQASVSDLK
                     SLDLSQYDGIISTVNLDIDSPYLTVNPLLPDSDISYVAQFLNTKSTFQETHDKSSNMI
                     DKDDVHVETKDVDGNTSFENEQTSYLTSVFEKHLSDEKSEQLLHHMRSGLTLLDSVKI
                     VSTEVKQWQTYIADYLYQCDVINDPTSFAELLEQRLIDNPGWILSPYPVAIPHLRDNM
                     IKHPMILITVLEEPLTLPSIQNDNQTIKYMISMFISDNDFMASLVSDLSEFLSLKLES
                     IDTFMENPQELETLLRNKFLERIKKQFI"
     gene            10077..10511
                     /locus_tag="O0293_000679"
     CDS             10077..10511
                     /locus_tag="O0293_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCY0279578.1"
                     /translation="MSELFSNDNIFLNVNVNSQNEAIEKAGKALVDSGAVTDAYIQAM
                     KDREQVVSTFMGNGLAIPHGTDEAKTNVIHSGLTLLQIPEGVDWDGEVVKVVVGIAGK
                     DGEHLDLLSKIAITFSEEENVDRIVQAKSAEEIKQVFEEADA"
     gene            10511..11617
                     /locus_tag="O0293_000680"
     CDS             10511..11617
                     /locus_tag="O0293_000680"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCY0279579.1"
                     /translation="MKAVHFGAGNIGRGFIGYILADNNVKVTFADVNEEIINALAHDH
                     QYDVILADESKTTTRVNNVDAINSMQPSEALKQAILEADIITTAVGVNILPIIAKSFA
                     PFLKEKTNHVNIVACENAIMATDTLKKAVLDITGPLGNNIHFANSAVDRIVPLQKNEN
                     ILDVMVEPFYEWVVEKDAWYGPELNHIKYVDDLTPYIERKLLTVNTGHAYLAYAGKFA
                     GKATVLDAVEDSSIEAGLRRVLAETSQYITNEFDFTEAEQAAYVEKIIDRFNNSYLSD
                     EVTRVGRGTLRKIGPKDRIIKPLTYLYNKDLERTGLLNTAALLLKYDDTADQETVEKN
                     NYIKEHGLKAFLSEYAKVDDGLADEIIEAYNSLS"
     gene            complement(11936..19372)
                     /locus_tag="O0293_000681"
                     /pseudo
     CDS             complement(11936..19372)
                     /locus_tag="O0293_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
     gene            complement(19649..21004)
                     /locus_tag="O0293_000682"
     CDS             complement(19649..21004)
                     /locus_tag="O0293_000682"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCY0279580.1"
                     /translation="MGKYFGTDGVRGVANQELTPELAFKLGRYGGYVLAHNKGEKHPR
                     VLVGRDTRVSGEMLESALIAGLISIGAEVMRLGIISTPGVAYLTRDMGAELGVMISAS
                     HNPVADNGIKFFGSDGFKLSDEQENEIEALLDQENPELPRPVGNDIVHYSDYFEGAQK
                     YLSYLKSTVDVNFEGLKIALDGANGSTSSLAPFLFGDLEADTETIGCSPDGYNINEKC
                     GSTHPEKLAEKVVETESDFGLAFDGDGDRIIAVDENGQIVDGDQIMFIIGQEMHKNQE
                     LNNDMIVSTVMSNLGFYKALEQEGIKSNKTKVGDRYVVEEMRRGNYNLGGEQSGHIVM
                     MDYNTTGDGLLTGIQLASVIKMTGKSLSELAGQMKKYPQSLINVRVTDKYRVEENVDV
                     KEVMTKVEVEMNGEGRILVRPSGTEPLVRVMVEAATDEDAERFAQQIADVVQDKMGLD
                     K"
     gene            complement(21031..21963)
                     /locus_tag="O0293_000683"
     CDS             complement(21031..21963)
                     /locus_tag="O0293_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HCY0279581.1"
                     /translation="MLESKWGLRFIAFLLALFFFLSVNNVFGNIFNTGNLGQKSSKTI
                     QDVPVEILYNTKDLHLTKAPETVNVTISGPQSKIIKIENPEDLRVVIDLSNAKAGKYQ
                     EKYQVKGLADDIHYSVKPKLANITLENKVTKKMTVQPDVSQSDIDPLYKITKQEVSPQ
                     TVKVTGGEEQLNDIAYLKATFKTNKKINGDTKDVAEVTAFDKKLNKLNVSIQPNEVNL
                     QVKVEPFSKKVKVNVKQKGSLADDKELSSIDLEDKEIEIFGSRDDLQNISEVDAEVDL
                     DGISESTEKTVKINLPEHVTKAQPSETKAYINVK"
     gene            complement(21965..22774)
                     /locus_tag="O0293_000684"
     CDS             complement(21965..22774)
                     /locus_tag="O0293_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HCY0279582.1"
                     /translation="MDFSNFFQNLSTLKIVTSILDLLIVWYVLYLLITVFKGTKAIQL
                     LKGILVIVIGQQISMILNLTATSKLFDIVIQWGVLALIVIFQPEIRRALEQLGRGSFL
                     KRYTSNTYSKDEEKLIQSVSKAVQYMAKRRIGALIVFEKETGLQDYIETGIAMDSNIS
                     QELLINVFIPNTPLHDGAMIIQGTKIAAAASYLPLSDSPKISKSLGTRHRAAVGISEV
                     SDAFTVIVSEETGDISVTFDGKLRRDISNEIFEELLAEHWFGTRFQKKGVK"
BASE COUNT         6631 a         4022 c         3579 g         8604 t
ORIGIN      
        1 ttcaatcaat tgttcttcca aatcactata atcttgtgtg tcctttttaa ataattcaaa
       61 cataatatta ttggataaat tttcattata tattaatcgc tcgtacatac cagaagcgct
      121 agagttgata aatctattca gatcgttttg tatttcgttc aaatctatat tccctttact
      181 atcgtaatat gaataattac tcattaattt ttttaagaaa tctctcaaac caaagtaaag
      241 ataaatattt gaatattttc tggcattatc gtagtctttt acatctcttt tatagtgatc
      301 tatcaacata ttcaattcga aattatatcc atgattaaaa tcaaactcgc cattgtcata
      361 ttttctttga aattcaaact tgtatatttc tgcaacttta tcatcgcaat tttcatctat
      421 atagttttca accaaataat aagcacgctc aagagaaatt tcaccataac tttgtataaa
      481 agaggttaag tactctgttt tctttaaaat ttcttctcct ttcttagttg gtatatatac
      541 atatggtaaa tctaagttat taataatatg aaaattgtcg tcaatacgtt taattaagtc
      601 aggtttatta cctgttgttt ttataccaaa actttttaaa atactttgta attcaggtct
      661 tgtcttttta tatatactaa cttcaaaatc attagtcgtt gttaataact cagcttcaat
      721 taattttttt attgaattca taatttgaac atcattatta gcaaggcact tattagcaaa
      781 tatctcttct ccaaccaatc tatttacatt agtgtgtaat actaataaat cacgtgcatt
      841 tacgctcact agctttcatc tcctcatttt tgcttttcaa atttattaag tatgaataaa
      901 aaatacatcc gaacaaacaa aatctctata ttctaattcg atttacatag attatacact
      961 aaaaaaatga ttctcaatgc aaatgaatgt gtctctcata gtctaaaaac tcgtatgttt
     1021 cgaattttat gattcattga taatagtacc agttaaaaga actaagattg taattatccc
     1081 cccttaaaat tcaagaatat attttcttaa tctatcagca agaggttata tttcaaagtg
     1141 tttattttta aaacaacatc taaaagccat tttcataata atgttaaatc attagaatgt
     1201 ataaagattc caattaataa aaatagataa aatgcaataa agttccaaat acttattatt
     1261 attcatcaca aattacgtga tgccctctac aacactaaat caaggatgat atttatttta
     1321 tatacattag cattcattgt ttgcttaccc acattatcct gtttagtctt ttttataact
     1381 ctagtcttga atgtcatatt tacaaagaaa aaaacactaa aatatttaaa actaacacta
     1441 tttattcact ttgttctttt atagcacctt ttatgtttat tttcttgtat tcaataaata
     1501 gtatggagat gataatttaa agtagatata tttgaagtat actatagagt aaattatatt
     1561 ggggaaatat gtaaacgaac cggttgattt tatagtggat taacacttca tagagaatat
     1621 aatcaagaac aaaaacagtc aataggtgtg aattttttta aagacggatc tgttgctact
     1681 aaaaaaagaa gtatacaatt caatagttaa accttaaaac aagaaatatt attcaaaatc
     1741 aatgaatttc ctatcttatt agttttaaca atatttattc tcatagagtt tgtccaatta
     1801 aatatagatg attcaaatat ttataagcat acaaaagagc agtaagacat tttctaatag
     1861 aaaataactt tactgctctt tgtacattac tcatcaacta ttgtaaatta aataataaat
     1921 aactacctat tttattattc agctaaaaaa tgctctgcta aatattttgc tgcgccatcc
     1981 tcttcattcg tgtatgccgt tacatctgaa gttaatgctt ggatttcagg gcgtgcattt
     2041 ttcatagcaa ctgtataatg accaaattca aacattgctc tatcattgtc gctatctcca
     2101 ataactaacg tttcttcttg atgaatacca aaatgtgcaa tcatttcttt aatgcctgta
     2161 cctttatcag tttgataagc cattgtttcc gcattaaatc ttgatgaatt tgaaacacta
     2221 atctgtagtt gcacatgatt ttgctttaat tcatctctaa atgctgttat tttttctaaa
     2281 ttagaactga atagataaat ttttgaatat tcaccttcag gaaacttagt tacccaatct
     2341 atcttaccag caagcgcatc ttgtcttgaa gaccattcac tatgacttac gccattaata
     2401 ggatcttgac tacgaatcat atctcgcatc catgtttcat cttcttttaa agaaactcta
     2461 ttaccttcaa aaggaaatac ctcataataa atttgttggc gcttagctaa attagtaatt
     2521 tgttgcactt gagccaatga taaaccatgc ttgaagataa tttctccatc tacttctcca
     2581 attgttccat ttgaactaat gatgccatta accgcaaaat cttgaggtac aagttgatgt
     2641 atttcagaat gcgaacgtcc tgttgccaaa aatactttat atcctttctc tctcaattga
     2701 ttaatgacat ctttcgtata cgttgatgct tgattatttt catgtaaaat tgttccatcc
     2761 atgtccaaaa atattgcttt tacgttatcc atattgccct ccactgtcca ttttatctta
     2821 attaaatttt gtcattatta cttattattt gcaaaatatt tactctaaca ttgccataga
     2881 aaatcgatta ttccatcttt gaactgctac atttttctgg aaaaatcgtg acatgttttc
     2941 agaagttaat atgtcttcta cagcgccttg ttgaatactt tggccatctt ttagcagtaa
     3001 aattttggaa aagttagcag ttatttcttc aataaagtgc gtcacataaa tcatcgcaag
     3061 cgttggatat gaatctgaca atgagtcaag tatacttaac aacgattctc gtgcaataaa
     3121 gtctaaacca gctgctggct catctaaaat taaaacctgg ggttgcccca ttaaagctcg
     3181 tgcaatcatc actcgttgtt tttcaccggt agataaataa ccaatatatt gttgcgcttt
     3241 agcaaacatt ccaactaatt taagtaattg atgtgcttca ttacgtatct catcatcaat
     3301 atcttgataa acaccaattg atttaaaggc accgcttatc accacatcga ttactctttc
     3361 accctcttga aacttttcca gtaaactatg agatacaaaa cctatatgtt gtcgtacagt
     3421 ctctgcagaa taccctacct tgcctggcat tttaccgaaa aggttaacag ttccagatgt
     3481 tgcaggctca tacgcattta aaatatttag aagtgttgtc ttgccagcac cattcaaccc
     3541 atataatatc catttatcac ctttagcaat ttgccaagaa atctttttta aaattgtttt
     3601 tccttgcttc attcgcccaa tttgatctaa ttgaattaac atgcgaggtc acccctttat
     3661 tgctactcca tacttctcat aaaatcatat taacataaca cccttaattg tcagactatt
     3721 taaataaata aaacacttca tttttacgca tttctgccaa attaagatga agtaaaagct
     3781 aagtcgacct aaaaaagcac ccttctagtc gattaatcta aaagggtgcc atatacttta
     3841 attttaatac atgattgatt ctaaaaaagt gaattattcc acagtaactg atttagcaag
     3901 gttacgtggt ttatcaacat ctaaatctct gtgtaatgct gcatagtatg aaattaattg
     3961 taatgcaacc actgatacta atggcgttaa caattcatgt acatgaggaa tgacataagt
     4021 gtcgccttct ttttcaagac cctccataga aataatacat ggatgtgcac cacgtgctac
     4081 tacctcttta acgttaccac gaattgataa attaactttc tcttgtgttg ctaaacctac
     4141 aactggtgta ccttcttcga ttaaggcaat tgtaccatgt ttaagttctc caccagcaaa
     4201 accttctgct tgaatgtaag aaatttcttt aagttttaac gcaccttcta aacttacgtt
     4261 atagtcaata gtacgtccga taaagaatgc attgcgtgtt gtttctaaga aatctgtagc
     4321 aatttgttcc ataattggtg catcgtcaac aattgcttct attgctgttg ttacttttgc
     4381 taattctctc aataaatcaa tatctgcttc acgaccatgc tcttttgcaa cgatttgaga
     4441 caagattgat aatactgcaa tttgtgcagt atatgctttt gtagatgcaa ctgcgatttc
     4501 aggacccgcg tgtaataaca atgtgtggtc tgcttcacgt gataaagttg aacctgcaac
     4561 attagtgatt gttaatgatt tatgacctaa tttattagtt tcaactaata cggcgcggct
     4621 atctgcagtt tcacctgatt gagaaatata aacgaacaat ggtttttcag ataataatgg
     4681 catgttgtag acaaactctg atgcaacgtg tacttcagtt ggtacgccag cccatttttc
     4741 taaaaattct ttacctacta aacctgcatg gtagcttgta cctgctgcaa taacgtaaat
     4801 gcggtctgct tctttaacat cattgatgat gtcttgatca attttcaagt taccttctgc
     4861 atcttgatat tcttgaataa tacgacgcat tactgctggt tgttcatgaa tttcttttaa
     4921 catgtagtgt gcataaacac ctttttcagc atctgatgca tcaatttcag caatatatga
     4981 atcacgttct acaacgtttc catctgcatc tttaataata acttcatctt ttttaacaat
     5041 aacgatttca tggtcatgga tttctttata ttcgcttgtc acttgtaaca ttgcaagtgc
     5101 gtctgatgcg ataacattga aaccttcacc aacacctaat aataatggtg atttattttt
     5161 agcaacatag attgtgtctt tgtcttcagc atctaataaa cctaatgcat atgaaccatg
     5221 taataatgac acaacttttg taaatgcttc ttcagttgaa agtccttgat ttgaaaagta
     5281 ttcaactaat tgaacgataa cttctgtatc tgtttctgaa atgaatgata caccttgtaa
     5341 gtattcacct tttaactctt catagttttc aataacaccg ttatgaacta gagtaaaacg
     5401 gccatttgat gattgatgtg gatgagagtt ttcatgattc ggtacaccgt gtgttgccca
     5461 acgtgtgtga ccgattccaa caggtccatc aaaatcgcta ctatcagcaa ctttacgtaa
     5521 ttctgcaata cgaccttttt ctttaaatac agttgtatta tcatcattta ctactgcgat
     5581 acctgcagag tcataacctc tgtattctaa tttttctaaa ccttttaata ataattcttt
     5641 ggcattatca tagccaatat aaccaacaat tccacacata acgacatttt cctccatatt
     5701 ggaatagtac gcgtaaatta tgatttattg ccgataattt agattgacaa tctgctttca
     5761 taatataaat aggaacatgc tatcatcgca ttcatccata acaaattaag catagttatt
     5821 tttacaacta tacaaattgc tcacactgta ctttccatat taatattttt tatattcaat
     5881 ttctggcgat cttattaact ttgtccatta agtcacccta atgttttact taataagcta
     5941 acgaatgagc cacatccggg atagcatccg ccgatctatt cgatcactat cctcctcgtc
     6001 tacaaataca tatattgcac tctataaagg ccactcatat attaaccttt aatcttcaaa
     6061 tacaaatatt tatttgcaca ggcgctttaa ctgtactgcc gaactttccc cctttccatt
     6121 aatcattatt gtacaacggt gttgttttgt tttgcaaata ttttcacaat aaaattttaa
     6181 aaatcctaaa acaatttttt tgttttactt tttcaaaata tctatactgt cacattgatg
     6241 acactttatt taattttgtc acatttattt tgacaaagtt gatttttgtt tatattgagt
     6301 aacaagtaac ctctctatac actatatata gtcacatata ttaaaaaaga ggtgtaaaca
     6361 tgtcacaaac tgaagagaaa aaaggaattg gtcgtcgtgt tcaagcattt ggatcattct
     6421 taagtagtat gatcatgcca aacattggcg ctttcatcgc ttggggtttt atcgcggcca
     6481 tttttataga taatggatgg ttacctaaca aagatttagc tactttagca ggaccaatga
     6541 ttacttattt aatcccatta ttgatcgcat ttagtggtgg tcgtttaatt tatgatttac
     6601 gtggtggtat catcgcagca acagcaacta tgggggtcat cgttgcatta cctgatacac
     6661 caatgttact tggtgcaatg attatgggtc cacttgttgg ttggttaatg aagaagaccg
     6721 accaattgat tcaacctaga acgccgcaag gttttgaaat gttatttaat aacttttctg
     6781 ctggtatttt aggatttatc atgacgattg caggatttaa aattttagca ccacttatga
     6841 aatttattat gcatatttta tcagtggcag ttgaagcatt agtacatgca cacctacttc
     6901 cacttgtaag tattttagta gagcctgcaa aaattgtatt tttaaacaat gcgattaacc
     6961 atggtgtatt cacaccgctt ggtgcagatc aagctgcaaa agctggtcaa tcaattttat
     7021 acacaattga atctaaccct ggaccaggtt taggtatctt acttgcttat atgatttttg
     7081 gaaaaggcac tgcaaaagca acgtcatatg gtgctggaat tatccacttc ttaggtggta
     7141 ttcatgaaat ttatttccca tatgtattaa tgcgtccttt attatttatc gctgttattt
     7201 taggtggaat gactggtgtt gcaacttatc aagcaacagg atttggattt aaaagtcccg
     7261 cgtcaccagg ttcatttata gtttactgtt taaatgcacc tagaggtgaa ttcttgcaca
     7321 tgttactcgg tgtcttcctt gctgcacttg tatcattcgt tgtagctgct ttaattatga
     7381 agttcactag agaaccaaag caggatttag aagctgcgac agctcaaatg gaaaatacta
     7441 aagggaaaaa atcaagcgtt gcttctaagt tagtatcttc tgataaaaat gttaatacag
     7501 aagaaaatgc tagtggtaat gttagtgaaa catcttcatc agatgatgat cctgaagcgc
     7561 tattggataa ttacaacact gaagatgttg atgcacacaa ttacaataat ataaatcatg
     7621 ttatttttgc ctgcgatgcg ggtatgggtt ctagtgcaat gggtgcaagc atgttacgta
     7681 ataaatttaa aaaggcgggc attaatgata ttacagttac aaatactgcg attaatcaat
     7741 tgccaaaaga tgctcaatta gttatcactc agaaaaaact aactgatcgt gctattaaac
     7801 aaacaccaaa tgccatccat atttcagtgg ataatttcct taattcacca agatatgaag
     7861 aacttttaaa taatctaaaa aaagatgatc aagcataata attaaataaa ttaaaaaatg
     7921 gaggataccg ccatgttatt gagtacacgt gaaaaagaaa tgatagccct attgattaag
     7981 taccacggtc aatatatcac tatacacgac attgctcagc aacttgcggt gtcctctcgt
     8041 actattcacc gtgaattaaa aggtgttgaa gcatatttaa cttcattttc attaacttta
     8101 gaacgcgcaa acaaaaaagg gctacgcatt gctggcacag attctgattt aaacgatttg
     8161 aagcaatcga ttgcacaaca tcaaaccatt gacttatctg ttgaagagca gaaagtaatt
     8221 attatatacg ctttgataca agccaaggag ccagttaaac aatatagttt agcgcaagaa
     8281 attggcgttt ctgtccagac tttagcaaag atgttagatg atttagagct tgatttaaat
     8341 aagtaccaac tatctttatc tcgaaagcgt ggcgaaggca tttacttggt aggtactgaa
     8401 tcaaagaaac gtgaattttt aagtcaatta atggtgaata acttaaatag tactagcgtt
     8461 tattcagtaa ttgaaaatca ttttgtcttt cattcattaa atcaaatcca caaagacttt
     8521 gttgacttag agcgcatttt taatgttgaa agactattaa tggactacct aagtgcctta
     8581 ccctaccaac ttaccgaatc aagttattta actttaactg tccatatcgt gctctccatt
     8641 tcacgtataa aaaatggaga gtatgtcgca ttaaacgatg atatttatga ttctgtacaa
     8701 aacacatttg aacacaaagt agcaagcgaa cttgctgata aacttggtca aatatatgac
     8761 gtcacgttta atcaggcaga aattgctttc attactatcc atttacgtgg agctaaacga
     8821 aaaaatctta atgatacatc attaaataat cgttgtgaag aaaacaaaat taaagcgttt
     8881 gttaacaaag tagaaatgat ttccggtatg acatttgcag atttggatac tttagtagat
     8941 ggactgacgc tacaccttaa tcctgcaatc aatcgtttgc aagctaatat cgagacctat
     9001 aatccgttaa cagacatgat taagttcaaa tatccaagac tatttgaaaa tgtaagatta
     9061 gctttaaatg attgttggcc tgatttgatt tttccagaga gtgaaattgc ttttatagtt
     9121 ttacactttg gtggctcgat taaaaaccaa ggtaatcgat ttttaaacat attagtcgtt
     9181 tgcagcagtg gtatgggaac tagtcgtcta ttatcaactc gtctagatca agtttttagt
     9241 gagattgagc gtattacaca agcatcagtc agcgatttga agtcactaga tttaagtcaa
     9301 tatgatggca ttatttctac tgtgaattta gacatcgact ccccctattt aacggtaaac
     9361 ccattattac cagatagtga tatcagttat gtcgcacagt ttttaaatac aaagtctacg
     9421 ttccaagaga cgcatgataa atcatcaaac atgattgata aggatgatgt tcatgttgaa
     9481 acgaaagatg ttgatggcaa cacatctttt gaaaatgaac aaacttcata cttaacttca
     9541 gttttcgaaa aacatttaag tgacgaaaaa tcagaacaat tattgcatca tatgcgttcg
     9601 ggtttaactt tgcttgattc agtgaaaata gttagtaccg aagttaaaca gtggcaaaca
     9661 tatatcgcag attatctata tcaatgcgat gtaataaacg atccaacgtc attcgctgaa
     9721 ctactagagc aacgattgat tgacaatcca ggatggatat taagtccata tcctgttgca
     9781 ataccacacc taagagacaa tatgattaaa caccctatga ttctaatcac agttttagaa
     9841 gaaccgttaa cattgcctag tattcaaaat gacaatcaaa caattaaata tatgatttcc
     9901 atgtttattt ctgacaatga ttttatggca tcactggtaa gtgacttgtc cgaattttta
     9961 agtttgaaat tagaatctat tgatactttt atggaaaatc cacaggaact tgaaacatta
    10021 ttaagaaaca aatttttaga acgaattaaa aaacaattta tttaggagtg tacaaaatga
    10081 gcgaattatt tagtaatgac aatatctttt taaatgtaaa tgttaacagc caaaatgaag
    10141 caattgaaaa agcaggtaaa gccttagttg atagtggtgc tgtaacagat gcttatattc
    10201 aagcaatgaa agatcgtgag caagtcgtat caacatttat gggaaatggc ttagcaattc
    10261 ctcatggcac agatgaagct aaaacaaatg tgattcactc aggtttaaca ttattacaaa
    10321 tccctgaagg cgttgactgg gatggcgaag tagttaaagt tgtcgtggga attgctggta
    10381 aagatggcga acatttagac ttgttatcta aaattgcaat tacatttagc gaagaagaaa
    10441 atgtggatcg tatcgttcaa gcaaaatctg cagaagaaat taaacaagta ttcgaggagg
    10501 cagatgcata atgaaagcag ttcactttgg tgctggtaac ataggtcgtg gtttcattgg
    10561 ttatattctt gcagacaaca atgttaaagt aacatttgca gacgtcaatg aagaaatcat
    10621 taatgcttta gctcatgatc atcaatacga tgttatttta gctgatgagt ctaaaacaac
    10681 gacgcgcgtg aataatgttg atgcaattaa ttcaatgcaa ccttctgaag cgttgaaaca
    10741 agcaattcta gaagctgata ttattacaac agctgttggt gttaacatac tacctattat
    10801 tgctaaatct tttgcgcctt tcttaaaaga aaaaacaaac catgttaata ttgttgcttg
    10861 tgagaatgct attatggcaa ctgatacatt gaaaaaagca gtacttgata ttactggccc
    10921 tcttggtaac aatattcatt ttgctaactc agcagttgat agaattgtac cattacaaaa
    10981 gaatgaaaat atattagacg ttatggttga gccattttac gaatgggttg ttgaaaaaga
    11041 tgcatggtat ggtccagaac taaaccatat taaatatgtt gatgatttaa caccatatat
    11101 tgagcgtaaa ttattaactg tgaatacagg acatgcatat ttagcgtatg ctggtaaatt
    11161 tgcaggtaaa gctacagttt tagatgcagt tgaagatagt tcaattgaag ctggcttacg
    11221 ccgtgtttta gctgaaacta gtcaatatat tactaatgaa tttgatttta ctgaagcgga
    11281 acaagctgct tatgttgaaa aaataataga tcgtttcaac aattcttatt tatctgatga
    11341 agtaacacgt gtcggacgag gtacattacg taaaattggc cctaaagata gaattataaa
    11401 accattaaca tatctttata ataaagattt agaacgcact ggtttattaa atacagctgc
    11461 attgttattg aagtatgatg atacagcaga ccaagaaact gttgagaaaa ataattacat
    11521 taaagaacac ggtttaaaag cgtttttaag tgaatatgct aaagttgacg atggcttagc
    11581 cgatgaaata attgaagcgt acaattcact ttcataattt attgagcttt gtttgaaaca
    11641 agaagtttcc aacgttattc gttaacaatc agtaataatg tagtagttcc cttgaattaa
    11701 caatattaaa tttctgaaca taaaaaatac tcccttcaac atagacactt aacttgtgtt
    11761 atgtatgaaa ggagtatttt tgcgttaata atttgtttta ttttcgagcc acagccacct
    11821 attcaatggc tattggtcat tactaaaaca aattcatatt aactgttaga cttggttact
    11881 tagtaaggaa tatttcccta tgaaataact agatgttcac attcttgaat aaattttatt
    11941 cttcagtttg ttggtctttc ttagtgaatc ttctaattaa gaatgccata cctgcaccta
    12001 gagctaattc agcatatggt aaatcgtcat tatgtgacat accagtatct ggtaaagttt
    12061 tagcttgttg tttagcttta ttaacttttc cttgttgagc tgattttgtc ttagcttggt
    12121 ggtcgtcagt gttagttaca ttaagcatat cttgattagc actattgctt ccatttgaaa
    12181 ctgtagctgg agatgcattg gcaccgtcgt tttgcgtagc tttattgttt gcagctgaac
    12241 caactgattt ttgcgtatca ttagtatctg ctgttgccgt atcatctttt tggctaacat
    12301 tagttgaagt cattttttct tttgcttcag aagatgcaga tgttgatggt ttattcgaaa
    12361 cttcagtatc agctttgctt ggcgatttat ctgcttcgtt agatgcaacg ttagtttcag
    12421 acttaagttg tcctgcatca gtttgatttg tcgtactttc ttctttatct tttgatgtat
    12481 tagaaggtac atttggttct gttatgtctg ctgaaggcaa tgtttcagtt gttgattcaa
    12541 ccatactttg atttgttgaa tcactaccat ctttttctgc cttagcttta ttttcagatt
    12601 ttggttgtgc aaccttgtca ttagttgatt gagattcagc actattattt acttcagcat
    12661 tttgttttga atcatttaca gatgcattat ctttgctatc agcagatgat gctgcttctg
    12721 tgctcgcagt tgttggagcc gttgctgttg atcctgttgg tgcattctcg tttgttgctg
    12781 tagttgtact attgttattt gttgtgcttt ctgctggcgt tgcattatca gtttctgtta
    12841 caggtttatc agttgtgccg ttattagttg attctacttc tggtttacta gttacatcgt
    12901 tatccattgt cggactgttt gttgatgcat ctacactaga attgttatta gcttgcggtt
    12961 tatcatttgc atcatcagtt gctgatgttg ctgttgtttc acctgttgcc gcatcactat
    13021 tatttggtgt tgtcggagaa gcgtctgctt tgccattagc tgtcgtctca gatacgttag
    13081 gttgtccagt attttctggt gttgcattag catttgaatt tgctgttgca tcattattat
    13141 ctataccatt attagtatca ttagcatctg gatcattctg aggcacaatc gcttcaattg
    13201 caggtatcgt tacattttgt aattcagcaa cttctgcatt tgtttgtgtt ttatctaatt
    13261 tatcagcaaa tctgtcaaaa tatctaccta aatccgtacg tgcaatttct ttcgccgatg
    13321 catctgcatc tgcattttta attatttcta tttgcttgtt aaccacttct ctgattgctt
    13381 ccaaagcatt tttcttaact tcaggattaa tacgttgtgc tttaagttgt tcaagcgcac
    13441 tatttttgac agtagcgatt tctgcatttg tagtttgatc agaaatatct tcagttgctt
    13501 ttgataaaat gtcttctaaa gcattcgtaa acgcttcttt ttcttcagtt gtagcatcag
    13561 cgttgacatt tacacctgct tcaatctggt ctagtgcagt ttctaattct tcgatagctt
    13621 tttgtttttc tgttgagtcg atttgaatgt tatcaaatgc ttcaagtcct tgagctttcg
    13681 ctttttcaac ttcagcagtt gttgttgcat cagtaatacc ttgtttagct tgatctgtaa
    13741 tttgtttaat cattgctaat gcttcagttt tttcttcagc agttaactgg tcactttgat
    13801 caatagattc tttcgtatct tctgctttaa cttcaatagc ttggttcgct ttaggtttaa
    13861 cagtagcatc tacttgaata gcatcaattg ctgctttacc ttgtgttaat gcatcatcaa
    13921 cgtcaccatt atccacacca ttattaatgc tttctaatgc agtttgaaca ttttggtcaa
    13981 cttgcttaat tgcttgttgc ttttcatctt gtgttgcatt agtgtcagct gaaatattat
    14041 ttttcttctg atctgcataa gcatataaat ctgttgtagc tgattctttt ttacctgttg
    14101 gaattgtgta atcgttaata ttatctaagt cattatgaat ttgaacttca atgtcatctt
    14161 tagaagtagc ttgattaaca ttttgatccg cagtttgttt taattcagca agtttttgtt
    14221 tcgcttcagc aatttcactt gaagtcgatg cgttagagtt atccgcttcg tttactttag
    14281 cattatatgc atcttcaatt ttagctaaag catctttttt gtactcacta aatgttttaa
    14341 ctgcattaat tttagctttt ccttctttaa ctgcattatc aacatattca tttgttgatg
    14401 attgatcgac atttgttttc gcttgattta attcagtatc aacttcttgt tttgcatcat
    14461 taatttcttg ttgtgatgca tttggtgttt gttctatttg tgttttctta tcagctgcag
    14521 cttgatctaa ttctttttta cctgctggtt tcttaacagg atttgcatga agttgttgaa
    14581 ctttttgtac tgctgtatct ttagcagtag ttacatcacc tgtagtagtt gctgcattaa
    14641 tattatttaa accttcttca tatgctgctc taactggtcc aatatcgtta cctttttctt
    14701 cattagtagt ctcattatta ttaagtattt cagttatttt attttgcatt tcagttagca
    14761 attccgctct tgcattcgtt ttaacatctg ttgatgcttg aattgggtca attgcttgaa
    14821 ttgcattatc ttttgcagtg tttacatcat cgattgactg tgcattttca atattttgat
    14881 taccttgtgt taattgtgcg tctacttgtt gatttgcttg ttctttttct tcagtagtcg
    14941 catctgcagt ttgtgcaata agcgcttttt gttcgtttgc ttttgttgct aattcatctt
    15001 tcgcaacatc tttaattgtt gtatctgcag taataccttg aatatcagca actgcttgat
    15061 ctttaatttg cgtaacatca ttagttgttt gtgcatttaa gatatcttga tacgcttttt
    15121 ctttagcttt taaaactaaa tcttttgctg catttttctc ttcagttgta gcaccagttg
    15181 tattatcaat tgcttgattt tgagttgtca cagcttgatc aacatcattt ttagcatttg
    15241 atttaaccgc tgttgctggt tgcgtgcttt gaattgaatt ctttccagcg tctttcgcct
    15301 gatctacacc attatcatca gttgcagctg taatattatt tttcgcgtct gtaactgctg
    15361 ttgctaattg ttgaatagct gcttcttttt cttctgttgt agcgttctga tcattattga
    15421 taacatttgt ttgcgttgct tgtaattgat caatttcatc tttagccgct tgtttcttca
    15481 caacttttgg tgttaccgca ttaatcgctg cttctgcatt tgctttagct tcatcaactt
    15541 gtgcgttagt agttgctgct gaaatggctt gatttgcttt accattttca gtatttgctt
    15601 cagcatcagc tgcttgtttt tcttcatctg ttgcatctgg cgtagcttga atctcttgca
    15661 atttgttatt taaaattgct gtgatttcat tacgtgcagt tgctttttta ttaactgttg
    15721 gtgttacttg atcaatacta ttttcacctg tcgttttcgc ttggtctaca tcattttgac
    15781 tattagcagc ttcaatgttg ctatttgctt gtgtcacagc attatctacg tccgcattag
    15841 ccgctgcaat ttcttcagca gtaatgtctt gcgtttgagc gattgctgtt ttacgttcat
    15901 tcgctttcgt agcaattgct tctttcgcat tatctttagt tgttgttgct ggctgaatcg
    15961 cttcaatttt agcaattgct gcttttttag ccgcttcaac ttccgcattt gtatgtgctg
    16021 catctattgc ggcatcagct gttgtttttt cagtttgaac ttgttattta gcagctgctt
    16081 tttcttcagt tgttgagccg ttatttccat caattgctgt ttcttgagct tgtactttat
    16141 ctgcaattgc ttgttttgct gctggtttaa catttgcatc aggtgtaatg gctgcgattg
    16201 tagcttcatt tgtagttttt gcattatcca catcattgtt tgctgcagca ttatctatat
    16261 cagcgtttgc agtaactact gcttgatcca ctttgtcttt cgctgcttgt tgttcttcag
    16321 tagtcgaatc attcattgct tcaattgctg ttttacgttc acttgctttt tgagcgattt
    16381 ccgcttttgc atccgatttc ttagttgtgg cagcttgaac ttgattaatt gcagcaatac
    16441 tattgtcttt agctgttgtt acatcactgt ttgtatttgc agcatcaaga tttgttcttg
    16501 cttcttgctt tttagtatct aattctgtat atgcagcttg tttttcttct gtagttgaag
    16561 cattgctatt ttgaatttct tgtttacgtg tattatatgc gttttctact tccgtatcag
    16621 ctgcaggttt aacttttgct tgtgtttgaa ttgcattgat tttatctaat acttttgatt
    16681 ttgtatcagc aacttcctgt tttgatttaa caacttggat gtcatgtaaa ccttgctttt
    16741 gagctgcatc tactgctgca tcagcttctg ctacttcttc atttgttgca tttgaaactg
    16801 tagcattttg agcttttcta gctgttgcag cttgtttaac ttcattatag gcatccattt
    16861 ttgtttgcgt agagtcagta atattttcaa tttttgagat ttcttcgttt ttaactcttt
    16921 ctaaatcttg tgcagtcgtt gtcgcttcaa ttgctttaac accagaaact ttagctgcat
    16981 taatacgttc aatagcttcc gctacttctt catttgttgt atcaggattt aaaggtgctt
    17041 gatcaatttg tgcttgaaca acttcgtcaa attcttcaag agctgctgct ttagctgttg
    17101 tagcaggtgt agtttgatta atatcattga ttgcttttgt tttaatggct tctacttgtg
    17161 catttgttgt tgctgcatca atatctttaa ttgcctttgt ttcaatttta ccaattttat
    17221 catttgctac atctttttca tcttgtaatg atgcatttga ctttttaatt tgttgtttac
    17281 gagttgtaac tgcttggata atatcttgtt tcgcttgagg tttaactgtt ggtgtaatca
    17341 catcttgttc taacactgcg ataccattat ctttttcagt tgtgacacct tgtgctgtag
    17401 tttgtttatt aatttcagcg attgcattat ttttatcatt gttaatttta gttacaagtg
    17461 ctgcaacttc atcttgcgta acttttttac tttgttgtgc tgctgtaacc ttttcttgag
    17521 ctttagtatc taattcagca attgctgctt gattatcaat taatgcagct tgtaatttag
    17581 ttactaaacc atcaatatcc gcttgagatg cacgatttgc agttttaaca tgattcgcat
    17641 cttcatttaa aatagtatct gcttgttgtt taagtttatt gtattctgca attgatgctg
    17701 ttgtgtaatg actattatca acttgtgagt ttacttgttg ttgcaacgca tctttgttca
    17761 tttcaaccgc tactgaaaat ggatctgcag ttagtgtaac ttctggttgc gcattattaa
    17821 ttacaacatc tgaagcagta cgatatgtta atttttcatt aaaatcaata tttttaggtg
    17881 tatcgatatt cgcaacatta actttatatg ataattttaa agatttatct gggaataaaa
    17941 cttctttagt gtgtgtacca cgtgccgttg tcacaccttg gcttgtaaat gtaactttat
    18001 ttgcattttg atcataatta acagtcatat ttttcaatac tgtactgtct tcattaccat
    18061 tagggaatgt tgtagttaat gaattattaa cgtaagttac accttgtggt aatgttactt
    18121 catatttaaa ttgatctgct tttaatgaag caccaaaatt accattgtta ccgatttcag
    18181 tattaatagt atattcttta ttgttagttg ctttcggtgc taaatctcta tttttgacat
    18241 ataaatgtga tcctgagaat agacctacat tatcaatgaa tgaatagtat ttataaccat
    18301 ctttatttgt tgtaattctt gaagcatcag caactgctgt attgtcagga ataaattgta
    18361 aatctaaacg atcagcatca gttggtactt taaataaatg tgcagtacca gtcttagcaa
    18421 catttacagt tgctaattca gtatttttct tagcatcttt aataactaaa tttgttgcac
    18481 cttgcttact attcgtttgc atagtagtaa agttaataat taattctgaa tctggtttta
    18541 catttacagt ttttgaaata ccgttaaagt taccatgatc tgtagaatca tttgcattca
    18601 cacgacctaa tgcagccacg tttcctttag cttgatagtt ttgagggtta ttcttatcaa
    18661 acatatcgct tcgtcttaat tctgagttaa cgaaaccaat cttaccgttg ttaattaatg
    18721 aataaccatt tactttatct gtaacagtta cagttggatc ttgtctattc tcatctgttg
    18781 atatggcagg atcatcaaat gttaatgtcg tattaatact gccttcacca gtattgctag
    18841 catttggatc ttgagtttgt gcgtttgctg ctacaggtgc tgctggttgc gctgctgctg
    18901 gagcattcgc tggctgtgtt tgatttgccg gtgttgcatt attattaggt gttgcttggt
    18961 tatttccttg acctgcttgg tttgccggtg ttgcttgatt tccaggttgt gcttgtgcag
    19021 cgttattcgg atcagcttga ttaccttgtc cagctggttg tgtatttggt tgtgctgctc
    19081 ctcctgctgg attagcctgt ccaccttggt ttgctggttg tactgctggt tgtccttggt
    19141 tggcaggtgc agctggctgt gctgtaggat tagcttgagc accagcattt gcgttaggct
    19201 gtgtattggc atcagctggt tgtgctggtt gattttgtgc aggctgattt tgctctgctg
    19261 cagacgctgt tgtcgggtta gtagatataa aagtaacagt ggcaattaaa gctgaaaaaa
    19321 taccgacatt aaattttctg atactaaatt tttgttgtct gaataaattc attaagtcat
    19381 cctcctggtt gattattctc gctgttaaat gatttcactt aatcaactgt taagataagt
    19441 agtagcatct gcgttaaaaa cacaaagcaa ctctatctaa ttaaaattaa ttttatcatc
    19501 attatatatt gagtaccagt gtattttata ttacatattg attactttgt ttttattttg
    19561 tttatatcat tttacgtttg tactataaat tatttctaca aacacaaaaa accgatgcat
    19621 acgcatcggc tcatttgtaa tacagtattt atttatctaa tcccatttta tcttgaacca
    19681 catcagctat ttgttgtgca aatctttcag catcttcatc agttgctgct tcaaccatga
    19741 cacgaactaa tggttctgtt ccagaaggtc ttactaaaat tcgaccttct ccattcattt
    19801 ctacttctac tttagtcata acttctttaa cgtcaacatt ttcttcaaca cgatatttat
    19861 ctgttacgcg tacgttaatt aatgattgtg gatatttttt catttgtcca gctaattcac
    19921 ttagtgattt accagtcatt tttattacag aagctaattg aataccagtt aataaaccat
    19981 caccagttgt attgtaatcc atcataacga tatgtccaga ttgttctcca cctaagttat
    20041 aattaccgcg acgcatttct tctactacat atctgtcgcc aactttagtt ttattagatt
    20101 taattccttc ttgttcaagc gctttgtaaa aacctaaatt actcataaca gtagaaacaa
    20161 tcatgtcatt attcaattct tgatttttat gcatttcttg accaataata aacataattt
    20221 ggtcaccgtc aacgatttga ccattctcat ctactgctat gattctgtct ccatcgccgt
    20281 caaatgctaa cccaaaatca ctttcagttt caactacttt ttcagctaat ttttcaggat
    20341 gtgtagagcc acatttctca ttgatattat atccatcagg actacatcca attgtttcag
    20401 tatctgcttc taagtcacca aataagaatg gcgctagtga tgatgttgaa ccatttgcac
    20461 catctaaagc aattttcaaa ccttcaaagt taacatctac tgttgatttt aaatagctca
    20521 aatatttttg tgccccttca aagtaatctg aataatgtac aatatcattg ccaactggtc
    20581 ttggtaattc tgggttttct tgatccaata atgcttcaat ttcattttct tgttcatctg
    20641 atagtttaaa accatctgat ccaaagaatt taataccatt atctgcaact ggattatgag
    20701 aggctgaaat cattacacct aactctgcac ccatatcgcg tgttaaatat gcaacacctg
    20761 gtgttgaaat aatacctaat cgcatcactt ctgcaccaat tgaaatcaaa ccagctatta
    20821 atgctgattc taacatttca cctgaaactc tagtatcgcg acctacaagt acacgtgggt
    20881 gtttttcacc tttattatgt gctagaacat agccaccgta tcttcctaat ttaaatgcca
    20941 attcaggtgt tagttcttgg tttgcgacac ctcttactcc gtctgtacca aaatattttc
    21001 ccattgttta ctctccttta atttagctat ttattttaca tttatataag ccttcgtttc
    21061 acttggttgt gctttagtga catgttctgg taaattgatt tttacagtct tttcagttga
    21121 ttctgaaata ccatctaaat ctacttctgc atcaacttcg cttatatttt gtaagtcatc
    21181 tcgactaccg aagatttcaa tttctttatc ttctaaatca atcgaactta actctttatc
    21241 atctgctaaa ctacctttct gtttaacatt tactttaacc tttttgctaa aaggctctac
    21301 tttaacttgt aaattcactt cattaggttg aatcgataca tttaatttat tcagtttttt
    21361 atcaaaagcc gttacttctg cgacatcttt tgtgtcacca ttaatctttt tattagtttt
    21421 aaaagtggct tttaaataag cgatatcatt caattgttct tctccacctg ttactttaac
    21481 tgtttgtggt gaaacttctt gctttgtaat tttataaagt ggatcaatat cactctgact
    21541 tacatcaggt tgaactgtca tctttttagt tactttgttt tcaagcgtaa tatttgctaa
    21601 tttaggtttt acagaataat gaatgtcatc agctaaccct ttaacttgat acttctcttg
    21661 atattttcca gctttagcat ttgataaatc aatcactact cttaaatctt ctggattttc
    21721 aatttttatt atctttgatt gtggtcctga aatagtcaca ttaactgttt caggcgcttt
    21781 tgttaaatgc aaatctttag tgttataaag aatttcaacg ggtacatctt gaatcgtttt
    21841 actagacttt tgaccaagat taccagtgtt aaagatattt ccaaaaacat tgttaacaga
    21901 taaaaagaaa aacaatgcca aaagaaaggc aataaatctc aagccccatt tactttctag
    21961 catattattt cacacctttc ttttgaaagc gtgtgccaaa ccaatgttca gcaagcaact
    22021 cttcaaaaat ttcgtttgaa atgtctcgtc gtaattttcc atcaaatgtt accgaaatat
    22081 caccagtttc ttcagataca ataacggtaa atgcatcaga tacttctgaa ataccaaccg
    22141 cagctctatg tcttgtaccc aaacttttag atatcttagg actatcagac aatggcaaat
    22201 aacttgctgc tgctgcaatc ttcgtgcctt gaataatcat tgcaccatca tgtaaaggtg
    22261 tgttaggtat aaagacatta attaaaagtt cttgcgaaat atttgaatcc attgcaatac
    22321 ctgtttcaat ataatcttga agacctgttt ctttttcaaa gacaattaat gcacctatac
    22381 gtcttttagc catatattgc acagcctttg aaaccgattg aatcaatttc tcttcatctt
    22441 tactatacgt attagaagta tagcgtttta aaaagctacc tctaccaagt tgttctaacg
    22501 cacgtctaat ttctggttgg aatattacta ttaaagctaa taccccccat tgaataacga
    22561 tatcgaataa tttagatgtt gcagtcaagt tcaatatcat acttatctgc tgaccaataa
    22621 caattactaa tatcccttta agtaattgta tcgctttagt tcccttaaag accgtgatga
    22681 gaagataaag tacataccaa actatcagta aatcaaggat actcgttaca atttttaacg
    22741 tactgaggtt ttgaaaaaag ttggaaaaat ccataacatc tcctccgggt aatatttttc
    22801 cataataccc attataccaa tcatttacat cactgt
//
LOCUS       DAKTIO010000023        22403 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.023, whole genome shotgun sequence.
ACCESSION   DAKTIO010000023 DAKTIO010000000
VERSION     DAKTIO010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22403
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.023"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(441..1301)
                     /gene="folD"
                     /locus_tag="O0293_000685"
     CDS             complement(441..1301)
                     /gene="folD"
                     /locus_tag="O0293_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCY0279583.1"
                     /translation="MVAKILDGKQIAKDYRQGLQDQVEALKEKGFTPKLSVILVGNDG
                     ASQSYVRSKKKAAEKIGMISEIVHLEETATEEEVLNELNRLNNDDSVSGILVQVPLPK
                     QVSEQKILEAINPEKDVDGFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEGKN
                     AVVIGRSHIVGQPVSKLLLQKNASVTILHSRSKDMASYLKDADVIVSAVGKPGLVTKD
                     VVKEGAVIIDVGNTPDENGKLKGDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLL
                     AEKMRRGIDS"
     gene            1502..1984
                     /gene="purE"
                     /locus_tag="O0293_000686"
     CDS             1502..1984
                     /gene="purE"
                     /locus_tag="O0293_000686"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCY0279584.1"
                     /translation="MKVAVIMGSSSDWKIMQESCNMLDYFEIPYEKQVVSAHRTPKMM
                     VQFASEARERGINIIIAGAGGAAHLPGMVASLTTLPVIGVPIETKSLKGIDSLLSIVQ
                     MPGGIPVATTAIGAAGAKNAGILAARMLSIQNPSLVEKLNQYESSLIQKVEDMQNELQ
                     "
     gene            1971..3095
                     /gene="purK"
                     /locus_tag="O0293_000687"
     CDS             1971..3095
                     /gene="purK"
                     /locus_tag="O0293_000687"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCY0279585.1"
                     /translation="MNFNKLKFGATIGIIGGGQLGKMMAQSAQKMGYKVVVLDPSEDC
                     PCRYVAHEFIQAKYDDEKALNQLGQKCDVITYEFENISAQQLKLLCEKYNIPQGYQAI
                     QLLQDRLTEKETLKSAGTKVVPFISVKESTDIDKAIETLGYPFIVKTRFGGYDGKGQV
                     LINNEKDLQEGFKLIETSECVAEKYLNIKKEVSLTVTRGNNNQITFFPLQENEHRNQI
                     LFKTIVPARIDKTAEAKEQVNKIIQSIHFIGTFTVEFFIDSNNQLYVNEIAPRPHNSG
                     HYSIEACDYSQFDTHILAVTGQSLPNSIELLKPAVMMNLLGKDLDLLENEFNEHPEWH
                     LHIYGKSERKDSRKMGHMTVLTNDVNQTEQDMYAKFEGSN"
     gene            3099..3803
                     /locus_tag="O0293_000688"
     CDS             3099..3803
                     /locus_tag="O0293_000688"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCY0279586.1"
                     /translation="MTLLYEGKAKRIFSTNQENELRVEYKDEVTAGNGAKKDTMAGKG
                     RLNNQITSIIFKYLQENGIESHFIKQLSETEQLVKPVKIIPLEVVVRNIASGSITKRL
                     GFENGEVFREPLVEFFYKNDALNDPLITDDHVKLLNIASDEDIEILKSKALKINNVLK
                     QLMDAMNLKLVDFKIEFGKTETGQILLADEISPDTCRIWDKATNANFDKDVYRNNTGS
                     LIETYQIFLNKLEDLK"
     gene            3803..4066
                     /gene="purS"
                     /locus_tag="O0293_000689"
     CDS             3803..4066
                     /gene="purS"
                     /locus_tag="O0293_000689"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HCY0279587.1"
                     /translation="MKTIELHITLQPQVLDTQGQTLTRAVHDLGYAQVNDIRVGKVLY
                     MTVDEVSDEKVHNIITTLSEKLFANTVIEEYSYKVLDDEKENA"
     gene            4068..4739
                     /gene="purQ"
                     /locus_tag="O0293_000690"
     CDS             4068..4739
                     /gene="purQ"
                     /locus_tag="O0293_000690"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HCY0279588.1"
                     /translation="MKFAVLVFPGSNCDRDMFNAAIKSGVEAEYVDYRETSLSGFDGV
                     LIPGGFSFGDYLRSGAMASVAPIISEVKRLAAEGKPVLGVCNGFQILTEIGLLPGALL
                     HNDSHLFISRNEELEIVNNQTAFTNLYEQGEKVIYPVAHGEGHYYCTDEIYQQLKANN
                     QIILKYVNNPNGSYDDIAGIVNEKGNVCGMMPHPERALETLLGTDSGVKLFEAMVKSW
                     REQHV"
     gene            4732..6921
                     /gene="purL"
                     /locus_tag="O0293_000691"
     CDS             4732..6921
                     /gene="purL"
                     /locus_tag="O0293_000691"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HCY0279589.1"
                     /translation="MSKFIEPSVEEIKLEKVYQDMGLSDQEYEKVCDILGRQPNFTET
                     GIFSVMWSEHCSYKHSKPFLKQFPTSGDHVLMGPGEGAGVVDIGDNQAVVFKVESHNH
                     PSAIEPYQGAATGVGGIIRDIVSIGARPINLLNSLRFGELDNKQNQRLLKGVVKGIGG
                     YGNCIGIPTTAGEIEFDERYDGNPLVNAMCVGVINHDMIQKGTAKGVGNSVIYVGLKT
                     GRDGIHGATFASEELTEESESKRPSVQIGDPFVGKKLMEATLEAITFDELVGIQDMGA
                     AGLTSSSSEMAAKGGSGLHLRLEQVPTREPGISPYEMMLSETQERMLLVVEKGTEQKF
                     LDLFDKHELDSAVIGEVTDTNRFVLTYDDEVYADIPVEPLADEAPVYILEGEEKDYNT
                     SKNDYTHIDVKDTFFKLLKHPTIASKHYLYDQYDQQVGANTIIKPGLQASVVRVEGTN
                     KAIASTIDGEARYVYNNPYEGGKMVVAEAYRNLIAVGATPLAMTDCLNYGSPEKKEIY
                     QQLIDSTKGMAEACDILKTPVVSGNVSLYNETKGTSIFPTPVVGMVGLIENVNYLNDF
                     EPQVGDKLYLIGDTKDDFGGSQLEKLIYGKVNHEFESLDLSSEVEKGESIKTAIREGL
                     LSHVQTVGKGGLLITLAKLSAHYGLGLKSSIDITNAQLFSETQGRYVVSVKSGKTLNI
                     DNAIEIGLLTDSDNFKVTTPYTEISENVSDIKQIWEGAIAQCLTTQD"
     gene            6900..8384
                     /locus_tag="O0293_000692"
     CDS             6900..8384
                     /locus_tag="O0293_000692"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCY0279590.1"
                     /translation="MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIV
                     VSDQNELKGERGLGLLTEAIKDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHF
                     YDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKES
                     LRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQ
                     DIHAGEYVVINDKGITVKSYTHHTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKK
                     LAQESPVNADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQ
                     GVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFP
                     SFYGIDVSTTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVE
                     SFTGDYPAGLYDYEANYKAHLSHRQKQYISKNKHFFDSEGNLNV"
     gene            8377..9405
                     /locus_tag="O0293_000693"
     CDS             8377..9405
                     /locus_tag="O0293_000693"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCY0279591.1"
                     /translation="MSKAYEQSGVNIHAGYEAVERMSSHVKRTMRKEVIGGLGGFGAT
                     FDLSQLNMTAPVLVSGTDGVGTKLKLAIDYGKHDSIGIDAVAMCVNDILTTGAEPLYF
                     LDYIATNKVVPEVIEQIVKGISDACVETNTALIGGETAEMGEMYHEGEYDVAGFAVGA
                     VEKDDYVDGSEVKEGQVVIGLASSGIHSNGYSLVRKLINESGIDLASNFDNRPFIDVF
                     LEPTKLYVKPVLALKKEVSIKAMNHITGGGFYENIPRALPAGYAARIDTTSFPTPKIF
                     DWLQQQGNIDTNEMYNIFNMGIGYTVIVDEKDVSRALKILAEQNVEAYQIGHIVKNES
                     TAIELLGV"
     gene            9408..9974
                     /gene="purN"
                     /locus_tag="O0293_000694"
     CDS             9408..9974
                     /gene="purN"
                     /locus_tag="O0293_000694"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCY0279592.1"
                     /translation="MVKIAIFASGSGSNFENIVEHVESGKLENIEVTALYTDHQNAFC
                     IDRAKKHDIPVYINEPKQFDSKAAYEQHLVTLLNEDKVEWIILAGYMRLIGPDLLASF
                     EGKILNIHPSLLPKYKGIDAIGQAYHSGDTITGSTVHYVDSGMDTGEIIEQRKCDIRP
                     DDSKEQLEEKVKKLEYELYPSVIAKIVK"
     gene            9989..11467
                     /gene="purH"
                     /locus_tag="O0293_000695"
     CDS             9989..11467
                     /gene="purH"
                     /locus_tag="O0293_000695"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCY0279593.1"
                     /translation="MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVP
                     VRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLY
                     PFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYQEVLTRLRNDS
                     LDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIA
                     GAKQLHGKQLSYNNIKDADATLALVKKFDTPATVAVKHMNPCGVGIGDTIEQAFQHAY
                     EADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEALDILKQKKNVRLLEI
                     DMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPS
                     VKSNAIILSNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVEL
                     AAQHGIKAIIQPSGSIKDQDSIDMANKHGIAMVVTGTRHFKH"
     gene            11489..12736
                     /locus_tag="O0293_000696"
     CDS             11489..12736
                     /locus_tag="O0293_000696"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCY0279594.1"
                     /translation="MNVLVIGAGGREHALAYKLNQSNLVKQVFVIPGNEAMTPIAEVH
                     TEISESDHQAILDFAKRQNVDWVVIGPEQPLIDGLADILRANGFKVFGPNKQAAQIEG
                     SKLFAKKIMEKYNIPTADYKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTI
                     EAARSAIEIMYGDEEEGTVVFETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHD
                     EGPNTGGMGAYCPVPHISDDVLKLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDG
                     PKVIEFNARFGDPEAQVLLSRMESDLMQHIIDLDEGKRTEFKWKNESIVGVMLASKGY
                     PDAYEKGHKVSGFDLNENYFVSGLKKQGDTFVTSGGRVILAIGKGDNVQDAQRDAYKK
                     VSQIQSDHLFYRHDIANKALQLK"
     gene            complement(13001..13807)
                     /locus_tag="O0293_000697"
     CDS             complement(13001..13807)
                     /locus_tag="O0293_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCY0279595.1"
                     /translation="MYEQWKVRQSFVDDVNIITKLFLGIILFFFIIFVHNFDFMLYIT
                     ILMLVFLLMFNGTQFKITAIFITISIGFALLSSLFMILYGNGSHTILKFGFIHITTES
                     LVRGLHVSLRTIAISFFGILIALTSQVVMIFYSLMQHLKVKSKVAYAFMAAIRMIPLM
                     ISSLIQLRRSLKMRYQMIDAANYRGLKRFKHLIIPLLSQNIRKAHQLSVAMESKGFRD
                     GPRTYYYNVPFSYKDIIFIIVLIAIITMAYYCSITLPITGIDDVRLGRVG"
     gene            complement(13800..15200)
                     /locus_tag="O0293_000698"
     CDS             complement(13800..15200)
                     /locus_tag="O0293_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0279596.1"
                     /translation="MLKVSDLRLKYPSGQRKIFDHLNITIQDKEKVLLLGPSGCGKST
                     LLNVLSGIVPNLIELPMKYDELIVDPLSGVIFQDPDSQFCMPKVYEELAFVLENRQLP
                     REDMDALIINALNMVNLNVTPETYIKDLSGGMKQKLAIVETILQQSKTLFLDEPTAML
                     DVQATEDLWTKLIELWEDQTVVIVEHKVKHIWNHVDRVILMDYNGNIIADECPEIILQ
                     KYVHLLSEYGVWHPRAWEFAPSRVDFPTTNSHLLQFKNGRIIRGKSTLLSFSDLEIGL
                     GEWITITGANGSGKTTLLESIMQLIKYQGDVYFENQRLTKIKHAAKHMYLVYQNPELQ
                     FITNSVYDEINIHFNHLSKDQSDDETIQLLKLLDLQNVKDQHPYELSIGQKRRLSVAT
                     ALSSKADIIFLDEPTFGLDSHNTFQLIKLFQKRINLGQSIVMVTHDDEIIERYPSRRL
                     KISDGALLDCDGDTNV"
     gene            complement(15215..15790)
                     /locus_tag="O0293_000699"
     CDS             complement(15215..15790)
                     /locus_tag="O0293_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCY0279597.1"
                     /translation="MSKGLKLSEILVTVLISVVFAVIYNLWWFVYNGVQATGLHLEQL
                     TNGVWFMAAIVCYLIIPKPGIALLAEFAAGAGETIIMGRFDIPTIVYAFIQGLACELV
                     FAIFKYQSRSVMVAMLAGFCTAIAAFPIDYFYGYLNEVAGWNLTLFIVFRLISGAVVA
                     GVLSYLLVKALDKTGVTKLFRPAAKEDYDNL"
     regulatory      complement(15867..15967)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(16374..16508)
                     /gene="graF"
                     /locus_tag="O0293_000700"
     CDS             complement(16374..16508)
                     /gene="graF"
                     /locus_tag="O0293_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance-associated protein GraF"
                     /protein_id="HCY0279598.1"
                     /translation="MSNENQNKKAAEKAKEVEEKLKDKKEEKTEDINQTKQDIQDTLN
                     "
     gene            16660..17955
                     /locus_tag="O0293_000701"
     CDS             16660..17955
                     /locus_tag="O0293_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279599.1"
                     /translation="MSFLRKHAEIIFSYLIGIVSLFTGLIILINLPLIKQLNGGKKVD
                     THVHNVWEFLNAFFSEIIKVMSRFIGNFPIVSAIVIIIFGILVMLIGHTLLRTIKYDY
                     DISIFFLVIGIMYFIITLILMTQVYGFFAVIFIIPFTIHIGYIVYKDELNQENVKNHF
                     MWIIVSYGISYLITQIALYGRIDANEIESIDILSVNAFFIIMWLLGQMAIWNFLFLRR
                     ALPLTKQELGEEEPELSRTSKGNVTNQTKIHLKQLQDKTTEYARKTRRSVDLDKIRAK
                     RDKFKKKVNDIIDIQEDDIPDWMRKPKWVKPMYVELFCGVVIFLFTFLEFNNRNALFV
                     SGDWKLSQTQYVIEWVTLLILLFIIIAYIATTLTFHLKGKFYYLQLFMGSILFFKLLT
                     EFINIMIHGLLLSVFITPTLLLMLLAIIISYSLQLRERP"
     gene            18380..19552
                     /locus_tag="O0293_000702"
     CDS             18380..19552
                     /locus_tag="O0293_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HCY0279600.1"
                     /translation="MKIATLNKGKETKYFNGYPLIEEEDIYSQDHLKEGDIFQIVTDK
                     SQYVATAYVGRQHKGLGWVLTYDKAQQINTAFFVKLFNTALAERDYYFNIDGTNAFRL
                     FNAEGDGVGGLTIDNYDGHLLIQWYSKGIYKFKYAILEAVRKVFDYKSIYEKVRFKDS
                     EYSGGFVEGDAPEFPIVIEENFTFYNVDLEDGLMTGIFLDQKEVRKKLRDQYAKERHV
                     LNLFSYTGAFSAIAASEASSTTSVDLANRSRSLTEENFGLNAIDPKSQYIYVMDTFDF
                     YKYAARHGHSYDTIVIDPPSFARNKKRTFSVQKDYDKLINGALNILSSEGTLLLCTNA
                     SVYPLKQFKNTIKKTLEESGVDYELTEVMGLPKDFKTHPHYKPSKYLKAVFVNIRH"
     gene            19606..20148
                     /locus_tag="O0293_000703"
     CDS             19606..20148
                     /locus_tag="O0293_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HCY0279601.1"
                     /translation="MGFKNNLTSNLTNKIGNSVFKIENVDGKGAMPTTIQELRERRQR
                     AEAIVKRKSLMSSTMSVVPIPGLDFGVDLKLMKDIIEDVNKIYGLDHKQVNSLGDDVK
                     ERIMSAAAIQGSQFIGKRISNAFLKIVIRDVAKRTAAKQTKWFPVVGQAVSASISYYF
                     MNKIGKDHIQKCENVIKNVM"
     gene            20302..20568
                     /locus_tag="O0293_000704"
     CDS             20302..20568
                     /locus_tag="O0293_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HCY0279602.1"
                     /translation="MEQNSYVIIDETGIHARPATMLVQTASKFDSDIQLEYNGKKVNL
                     KSIMGVMSLGVGKDAEITIYADGSDESDAIQAISDVLSKEGLTK"
     gene            20571..22289
                     /gene="ptsP"
                     /locus_tag="O0293_000705"
     CDS             20571..22289
                     /gene="ptsP"
                     /locus_tag="O0293_000705"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCY0279603.1"
                     /translation="MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAK
                     FNSAIEASKVELTKIRNNAEVQLGADKAAIFDAHLLVLDDPELIQPIQDKIKNENANA
                     ATALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVI
                     VGNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGD
                     MIIVDGLNGDVIVNPTEDELIAYQDKRERYFADKKELQKLRDADTVTVDGVHAELAAN
                     IGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYKEVLEAMGGKRVVVR
                     TLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMF
                     PMVATINEFREAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDF
                     FSIGTNDLIQYTLAADRMSERVSYLYQPYNPSILRLVKQVIEASHKEGKWTGMCGEMA
                     GDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQEEVIEL
                     VNNYVK"
BASE COUNT         7845 a         3236 c         4225 g         7097 t
ORIGIN      
        1 attgtaagct gacttttcgt cagcttcttt gttggggccc tgactagaat tgaaaacttg
       61 ttacaagctt attttcattc aattaactac tgtctatata aaattgttga gccaagtaca
      121 aagtgaaatg ctcccttcaa agccttcatt tattcaatgt ctactttgaa gggagcattt
      181 gatcgattaa tgtcccagat ttttcttgtt attcattgca attgcaaaat aatatttaat
      241 gtgggttaaa agactttatt aaaactcagt tattagggag tgggacagaa atgatatttt
      301 cgtaaaattt atttcgtcgt cccaccccgg caaggttgac tagaattgaa aaaagcttgt
      361 tacaagcgaa ttttcgttca gtcaactact gccattataa ctttgtagag catagaacat
      421 tgatttatgt ctcaggctct ttacgaatca atacctcgac gcattttttc tgcaagcaaa
      481 gtattattta ataccatagt aattgttaat ggaccaacgc caccaggaac tggtgtaata
      541 gctccagcaa tttctttaac cgcatcataa tcaacgtcac cttttaattt gccattttca
      601 tctggcgtat tgccaacatc gataattact gctccttctt tgaccacatc ttttgttact
      661 aaaccaggct taccaactgc actgacaatg acatcagcat cttttaaata tgatgccata
      721 tcttttgaac gagaatgtaa gattgttact gatgcatttt tttgaagtag taacttagaa
      781 actggttgtc cgacaatatg acttcgtcca attacaactg catttttacc ttctaaatca
      841 atatcagcat gttttaatat ttccatgatg ccgagcggtg tgcaaggtac aaaagtttgt
      901 tcatcgatat ataatttccc tatatttatt ggatgaaaac cgtccacatc tttttcagga
      961 ttgattgctt ctaatatttt ctgttcgcta acttgttttg gtaatggtac ttgtaccaaa
     1021 ataccactta cagaatcatc attatttagt ctatttagtt cgtttaatac ttcttcttca
     1081 gtagctgttt cttccaaatg tacgatttct gaaatcatac caattttttc agctgctttc
     1141 ttttttgatc taacataact ttgactagcg ccatcattac caactaatat aacggataat
     1201 ttaggtgtaa aacccttttc ttttagcgct tcaacttgat cttgtaaccc ctgtctgtag
     1261 tctttggcaa tttgtttacc atctaaaatt ttagcaacca taaaaacttc tcctcctatt
     1321 gtcaatatcc taactttgtt agtttataaa actaatttac atagcaaaag acgtaaattc
     1381 aaacaaaaaa tcaaaataaa gttcgatttt tgattgaaaa agcagaaatt gcttgttatg
     1441 ctatatctat aatatacaac taaagtaatt cacaattcaa acttttgaaa gggtgtacaa
     1501 agtgaaagta gcagtcatta tgggcagttc ttccgattgg aaaattatgc aagagagttg
     1561 taacatgttg gattattttg aaattccgta cgaaaaacaa gtagtatccg cacatcgtac
     1621 gccaaaaatg atggttcaat ttgcttctga agcgagagaa agaggtataa acattatcat
     1681 tgcaggcgct ggcggtgcgg cacatttacc aggtatggtt gcatcattaa cgacgctacc
     1741 agttattgga gtgccgattg aaacaaaaag tttaaagggt atagattctt tattatcaat
     1801 tgttcaaatg ccaggaggta ttccggttgc aacgactgca attggtgcag caggtgctaa
     1861 aaacgcaggt atacttgcag caagaatgtt aagtattcaa aatccttctt tagttgaaaa
     1921 actaaatcag tatgaatctt cgttaattca aaaagtggag gacatgcaaa atgaacttca
     1981 ataaattaaa gtttggtgcg actattggca ttattggtgg tggtcagctt ggaaagatga
     2041 tggcacaatc agctcaaaaa atgggttata aagtggttgt attggatcct tctgaagatt
     2101 gtccatgtag atacgttgca cacgaattta tacaagccaa gtatgacgat gaaaaggcac
     2161 tcaatcaatt aggacaaaaa tgtgatgtga ttacttatga atttgaaaac atttcagccc
     2221 aacaattaaa actattatgt gaaaagtaca atattccgca aggttaccaa gctatacagt
     2281 tattacaaga tcgcttaact gaaaaagaaa cattaaaaag tgctggtacc aaagttgtcc
     2341 cgttcatttc agtaaaagaa tctacagata ttgacaaagc aattgaaaca ttaggatatc
     2401 cttttattgt aaaaactaga tttggtggct acgatggcaa aggtcaagtt ttaattaaca
     2461 acgaaaaaga cttacaagaa ggttttaaat taattgaaac tagtgaatgc gtagctgaaa
     2521 aatatttgaa tatcaagaaa gaagtatctc ttactgttac aagaggaaac aacaatcaaa
     2581 tcactttttt cccattacaa gaaaatgagc atagaaatca aatacttttc aaaacaattg
     2641 ttccagcgag aatagataaa acagctgagg cgaaagagca agttaataaa attatccaat
     2701 cgattcattt cattggaaca tttacagttg aattttttat agatagtaac aaccaattgt
     2761 atgtgaacga gatagcacca aggcctcaca attccggaca ttattcaatt gaagcatgtg
     2821 attattcaca atttgatact catattttag cagttaccgg acaatcatta ccaaattcaa
     2881 ttgaattatt aaagccagca gtcatgatga acttactagg taaagattta gatttattgg
     2941 aaaatgaatt taatgaacat ccagagtggc acttacatat ttatggtaag tctgagcgta
     3001 aagatagcag aaaaatgggg catatgactg tactaacgaa tgatgtaaac caaactgaac
     3061 aagatatgta cgctaaattt gaggggagta attaaagcat gacattatta tatgaaggaa
     3121 aagcgaagcg cattttctca acaaatcaag aaaatgaatt aagagttgaa tataaagatg
     3181 aagttactgc tggaaacggg gctaagaaag acacaatggc aggtaagggg cgattaaata
     3241 atcaaattac ttctattata tttaaatatt tacaagaaaa tggaatagaa agtcacttta
     3301 ttaaacaatt atctgaaaca gaacaattag ttaagcctgt gaaaataatt ccattagaag
     3361 tagttgttcg taatattgct agtggatcta ttacaaagcg tttaggtttt gaaaatggtg
     3421 aagtttttag agaaccactt gtagaatttt tctataaaaa tgatgcgtta aatgatccgt
     3481 tgataacgga tgaccatgtt aaattgctca atatagcatc agatgaagat attgaaatac
     3541 taaaatccaa agcattaaag attaataatg tgttgaaaca attaatggat gctatgaatt
     3601 taaaattagt agattttaaa atcgaatttg gaaagactga gactggtcaa attttgttag
     3661 cggatgaaat atctccagat acatgtcgaa tttgggataa agctaccaat gcaaactttg
     3721 ataaagatgt atatagaaat aacactggat cactgattga aacatatcaa atatttttaa
     3781 acaaattgga ggatttaaaa taatgaaaac aattgaacta catatcacat tacaaccaca
     3841 agtattagat acgcaaggac aaacgcttac tcgagctgta catgacttag gttatgcaca
     3901 agtgaatgat attcgtgtag gaaaagtatt atatatgaca gtggatgagg ttagtgatga
     3961 aaaggtacac aacattatta caactctaag tgaaaaattg tttgcaaata cagtgattga
     4021 agaatatagc tataaagtgt tagatgatga aaaggagaat gcataaaatg aaatttgcgg
     4081 ttcttgtttt tccaggttcg aattgtgata gagacatgtt taatgctgct attaaaagtg
     4141 gtgttgaagc ggaatatgta gattatagag aaacatcact aagtggattt gatggcgtac
     4201 ttattcctgg tggattttca ttcggggatt acttaagatc tggggcaatg gctagtgtag
     4261 cgccgattat ttcggaagtt aaacgtcttg cagctgaagg taagccagta ttaggtgttt
     4321 gtaatgggtt tcaaatttta actgaaatag gcttattacc tggtgcatta ttgcataacg
     4381 attcacattt atttattagt agaaatgaag agttagaaat agtgaataat caaacggcat
     4441 ttacaaatct ttatgaacaa ggtgaaaaag ttatatatcc tgtagctcac ggtgaaggtc
     4501 attattattg tactgatgaa atatatcaac aattaaaagc taacaatcaa attattctga
     4561 aatatgtgaa taatccgaac ggttcatatg atgatattgc aggaattgtt aacgaaaaag
     4621 gcaatgtatg tggcatgatg ccacatcctg aaagagcttt agaaacgttg ttaggtactg
     4681 atagtggtgt gaaattattt gaagcgatgg taaaaagttg gagggaacaa catgtctaaa
     4741 tttatcgaac caagcgttga agaaattaaa cttgaaaaag tatatcaaga tatgggatta
     4801 agtgatcaag aatatgaaaa agtttgcgat attttaggca gacaacctaa ctttacagaa
     4861 acaggtatct tttctgttat gtggagtgaa cattgctctt ataaacattc taaaccgttt
     4921 ttaaagcaat ttcctacgtc aggtgaccat gtgcttatgg ggcctggtga aggtgcaggg
     4981 gtagtcgata taggtgataa tcaagccgta gtatttaaag tagagtctca caatcatcca
     5041 tcagcaattg aaccatatca aggggctgct acaggcgttg gtggaatcat tcgtgacatt
     5101 gtctctattg gggctagacc tattaatttg ttaaacagtc ttagatttgg agaattagat
     5161 aataaacaaa accaaagatt acttaaaggt gttgtaaagg gtatcggagg ttatggtaac
     5221 tgcattggta ttccaacaac tgctggtgaa atcgaatttg atgaacgtta tgatggcaat
     5281 ccacttgtta atgcaatgtg tgttggtgtt atcaatcacg acatgattca aaaaggcaca
     5341 gcaaaaggtg taggtaattc ggtcatttat gttggtttga aaactggtcg agatggtatt
     5401 catggtgcta cttttgcatc tgaagaattg acggaagaaa gcgaaagtaa acgaccttct
     5461 gtacaaatcg gtgatccatt tgtaggtaaa aaattaatgg aagcaacact tgaagcaatt
     5521 acatttgatg aattagttgg tattcaagat atgggtgctg ctggtttaac atcttcatcg
     5581 tctgaaatgg cggcaaaagg tggtagtggg ttacatttga gattagaaca agtgccaaca
     5641 cgtgagccag gtatttctcc ttatgaaatg atgctttcag aaactcaaga acgtatgtta
     5701 ctagttgttg aaaaaggtac tgaacaaaaa ttcttagatt tatttgataa gcacgaattg
     5761 gatagtgctg ttataggtga agttacagat acaaatcgtt ttgttttaac atatgatgac
     5821 gaagtttatg ctgacattcc agttgaacca ctagctgatg aagcacctgt atatatttta
     5881 gaaggagaag aaaaagatta taatacttct aaaaatgatt atacacacat cgatgttaaa
     5941 gatactttct ttaaattact taagcatccg actatagcat ctaaacacta tttatatgat
     6001 caatacgacc aacaagttgg tgccaatacg ataattaagc caggacttca agcatcggta
     6061 gtacgtgtgg aaggcacaaa taaggcaatt gcttcaacaa ttgatggtga agcgcgttat
     6121 gtatataaca atccatatga aggtggaaag atggtagtag ctgaagctta tcgaaattta
     6181 attgccgtgg gtgcaacacc attagcaatg acagattgtt taaattatgg ttctcctgaa
     6241 aagaaagaaa tctatcaaca gttgatagat tcaacgaaag gtatggcaga agcatgcgac
     6301 attcttaaga caccagtagt ttctggtaat gtatctttat ataacgaaac gaaaggtact
     6361 tctattttcc caacaccagt tgttggaatg gtaggtttga ttgaaaatgt aaattattta
     6421 aatgattttg aacctcaagt tggagataaa ttatatttaa tcggtgatac taaggacgac
     6481 tttggtggta gtcaacttga aaagttaatt tatggcaaag ttaatcatga atttgagtca
     6541 ttagatttga gttcagaagt tgaaaaaggt gaatcaatca agaccgctat tcgtgaagga
     6601 ctattatcac atgttcaaac agttggtaaa ggtggcttac tgattacctt agctaaacta
     6661 agtgcgcatt acggtttagg attaaaatct tcaatagata taacaaatgc acaattgttt
     6721 agtgagacgc aaggccgata tgttgtttct gttaaatcag gtaaaacttt aaatattgat
     6781 aatgcaatag aaattggact tttaacagat agtgataatt tcaaggtaac aacaccatat
     6841 acagagatta gtgaaaatgt ttcagatatt aaacaaatat gggaaggggc aattgctcaa
     6901 tgtttaacta ctcaggatta aacgaagaat gtggcgtgtt tggtatttgg aatcatcctg
     6961 aagcagcgca actaacatat atgggacttc atagtttgca acatcgtggt caagaaggtg
     7021 caggtatagt tgtttctgat caaaatgaat taaaaggcga gcgaggatta ggcttactaa
     7081 ctgaagcgat taaagatgat caaatggaac gattaaaagg atatcaacat gcaattggtc
     7141 acgtccgtta tgctacttca ggtaataaag gtattgaaaa tattcaaccg tttctgtatc
     7201 acttttatga tatgagtgta ggtatttgtc ataatggtaa cctcattaat gctaaatcat
     7261 tgcgtcagaa tttagaaaaa caaggtgcta tcttccattc gtcttctgat actgaagtca
     7321 ttatgcattt gatacgtcga agtaaagctc ctacttttga ggaagcgttg aaagaaagtt
     7381 tgcgaaaagt taaaggcggt tttacatttg cgattttaac taaagatgcg ttatatggcg
     7441 cagtagatcc aaatgctatc agaccacttg ttgtaggtaa aatgaaagat gggacataca
     7501 tccttgcaag tgaaacatgt gcaatagatg tgttaggtgc agaatttgtt caagatattc
     7561 atgcaggtga atatgtcgtg attaacgata aaggtattac agttaaatct tatacacatc
     7621 atacgacaac tgcaatttct gcgatggaat atatttattt tgctagacca gactcaacaa
     7681 tagctggtaa aaatgtccat gcagtacgta aagcttctgg taaaaaatta gcccaagaaa
     7741 gccctgtaaa tgctgatatg gtcatcggtg tacccaattc atcgctatca gctgcgagtg
     7801 gttatgctga agaaataggt ttgccatatg aaatgggact agttaaaaat caatatgttg
     7861 caagaacatt tattcaacca actcaagaat tacgtgagca aggtgtgaga gtgaagttat
     7921 ctgcggtaaa agatatagta gatgggaaaa atatcattct tgttgatgat tccattgttc
     7981 gcggtacgac aattcgacgc attgtgaaaa tgttaaaaga ttctggtgca aataaagtgc
     8041 atgtgcgtat agcatcaccg gaatttatgt ttccaagttt ttacggaatc gatgtttcaa
     8101 ctacggcaga attaatttct gcaagcaaat cacctgaaga aattaaagat tatattggcg
     8161 ctgattcatt agcatatcta tctgtagatg ggttaattga atcaattggt ttagattatg
     8221 acgcgccata tagtggctta tgtgtagaaa gtttcactgg agattatcct gcagggttat
     8281 atgattatga agcaaattat aaagcgcatt taagtcatcg acaaaagcaa tatatttcta
     8341 aaaacaaaca cttttttgat agcgagggaa atttaaatgt ctaaagcata tgaacaatct
     8401 ggtgtaaata ttcatgctgg ttatgaagct gtagaaagaa tgtctagtca tgttaaacgt
     8461 acgatgcgta aagaagttat cggtggttta ggtggattcg gtgctacatt tgatttatca
     8521 caattaaata tgacagcgcc agttttagtt tctggaacag acggagtagg tacgaaatta
     8581 aaactagcta tcgactatgg taaacatgat tcgataggta tcgatgcagt cgcaatgtgt
     8641 gttaatgata ttttaacgac aggtgcagaa ccattatatt ttttagatta cattgctaca
     8701 aacaaagttg ttcctgaagt tattgaacaa attgttaaag gtattagtga tgcatgtgtt
     8761 gaaacgaata ctgcacttat cggtggagag actgctgaaa tgggtgaaat gtatcacgaa
     8821 ggcgaatatg atgtagctgg atttgctgtt ggagcagttg aaaaggatga ctatgtagat
     8881 ggttcagaag tgaaagaggg acaagttgtt atagggcttg cgtcaagtgg cattcattca
     8941 aatggataca gtttagtgcg caaattaatt aatgaatcag gcattgattt ggcatcaaac
     9001 ttcgataatc gtccatttat cgatgtcttt ttagaaccaa ctaaattata tgtcaaacct
     9061 gtacttgctt tgaaaaaaga agtttctatt aaggcaatga atcatattac tggtggaggt
     9121 ttttatgaaa atattccacg tgcattgcca gccggatatg ctgctagaat tgatactaca
     9181 tcatttccaa caccaaaaat atttgattgg ttacaacaac aaggcaatat agacacaaat
     9241 gaaatgtata acatttttaa tatgggtatt ggctatacgg ttatcgttga tgaaaaagat
     9301 gtatcacgcg ctttgaagat tttagcagaa caaaatgtgg aagcctatca aattggtcat
     9361 attgtgaaaa atgagtcaac tgcaattgaa ttgttggggg tataacaatg gttaaaattg
     9421 cgatttttgc atcaggttca ggaagtaact ttgaaaatat agttgagcat gttgaatcag
     9481 gaaaacttga aaatattgaa gttacggcgc tatatacgga tcatcaaaat gcgttttgta
     9541 tagatagagc aaaaaagcac gatattcctg tttatattaa tgaaccaaaa caatttgatt
     9601 caaaagcagc gtatgaacaa catttagtaa cattattaaa tgaagataag gtagagtgga
     9661 ttattctagc tggctacatg cgtctaattg gtccagactt attagcttca tttgaaggta
     9721 aaatattgaa tatacatcca tctctattgc cgaaatataa ggggattgac gcaataggcc
     9781 aagcatatca tagtggcgat actattactg gttcgacagt acattatgtt gatagtggta
     9841 tggatacggg agaaattatt gaacagagaa aatgtgatat tagaccggac gattcaaaag
     9901 aacaattaga agagaaagta aaaaaattgg aatatgagtt atatccaagt gttattgcta
     9961 aaattgtaaa ataaggagta tgtatgtgat gaagaaagct attttgagcg tatcaaataa
    10021 aacaggtatt gtagagtttg ctaaagcgtt aacgcaatta aattatgaat tatattcaac
    10081 aggtggtact aaacgtatat tagatgaagc aaatgtacca gttcgttctg tttcagactt
    10141 aacacatttt ccagaaataa tggatggccg tgttaaaaca ttacatccgg ctgttcatgg
    10201 tggcatttta gctgatcgaa ataaaccgca gcatttaaat gaattatcag aacaacatat
    10261 agatttaatt gatatggtag tagttaattt atatccattc caacaaactg ttgcaaaccc
    10321 tgatgtgacg atggacgaag caattgaaaa tattgatatt ggtggtccaa caatgttacg
    10381 tgctgcagct aaaaactata aacatgtaac aacaattgta catccggcag attatcaaga
    10441 agtattgacg cgattaagaa acgattcgtt agatgagtca tatagacaat cattaatgat
    10501 taaagttttt gagcatactg cagaatatga tgaagcgatt gtacgtttct ttaaagggga
    10561 taaagaaact ttaagatatg gagaaaatcc acaacaatca gcgtattttg tgagaacttc
    10621 gaatgctaag cacacgattg caggcgctaa acaattacat gggaaacaat taagctataa
    10681 caatattaaa gatgcagatg ctacactagc tttagttaaa aagtttgata cacctgctac
    10741 agttgcggtt aaacacatga atccatgtgg tgttggtatc ggtgacacga tagaacaagc
    10801 atttcaacat gcgtatgaag cggatagtca atcaatattt ggtggaattg ttgcattaaa
    10861 ccgagctgta acacctgagt tagcagagca attgcatagt atctttttgg aagtcattat
    10921 tgcaccaaaa tttacagatg aagcattaga tattttaaaa caaaagaaga atgtaagatt
    10981 attagaaatt gatatgacta tagacagtaa cgaagaagag tttgtttcag tatctggcgg
    11041 atatttagtt caagataaag acaattatgt cgtgccaaaa gaagaaatga aagttgttac
    11101 agaagtagca cctacagatg aacaatggga agcaatgtta ttaggatgga aagttgtacc
    11161 atcagtaaaa agtaatgcaa ttattttaag taataataaa caaactgtag gtataggtgc
    11221 tggacaaatg aatcgtgtcg gtgctgctaa aattgcgtta gagagagcta ttgaaatcaa
    11281 tgatcatgta gcgttagtat ctgatggatt tttccctatg ggagatacag ttgaacttgc
    11341 agcacaacat ggtataaagg caattatcca accgagtggt tcgattaaag atcaagattc
    11401 aattgatatg gctaataaac atggtattgc aatggtagtc acaggcactc gacattttaa
    11461 acactaattt taaaggggcg taacataaat gaatgtatta gtaattggtg ctggtggacg
    11521 agaacatgca cttgcatata aacttaatca atcgaatcta gttaaacaag tgtttgtcat
    11581 tccaggtaat gaggcaatga cacctatagc tgaagtacac actgaaattt cagaatctga
    11641 tcatcaagcg atactagatt ttgctaaacg gcaaaatgtt gattgggtag ttataggtcc
    11701 agaacagccg ctaattgatg gattagcaga cattttacga gcgaatggtt tcaaagtgtt
    11761 tggtccaaat aagcaagcag ctcaaatcga aggctcaaaa ttatttgcta aaaagataat
    11821 ggaaaaatat aatattccaa ctgctgatta taaagaagtt gagcgaaaaa aggatgcttt
    11881 aacatatatt gaaaactgtg aattgcccgt tgttgtcaag aaagatgggt tagctgctgg
    11941 gaaaggcgtt attattgcag atactattga agcagccaga agtgctattg agattatgta
    12001 tggtgatgaa gaagaaggta ctgttgtatt tgaaacgttt ttagaaggtg aagagttctc
    12061 gctaatgaca tttgttaatg gtgatttagc agtacctttc gactgtattg cacaagatca
    12121 taaacgcgca tttgatcatg atgaaggacc aaatactggt ggtatggggg cttattgtcc
    12181 agtaccacat attagtgacg atgttttaaa acttacaaat gaaacaattg cacaacccat
    12241 tgcaaaggca atgcttaatg aaggttatca attcttcggt gtattataca ttggtgctat
    12301 tttaactaaa gatggtccaa aagtaataga atttaatgcc cgttttggtg atcctgaagc
    12361 tcaagtatta ttaagtcgca tggaaagtga tttaatgcag catattattg atttagatga
    12421 aggaaaacgt actgaattca aatggaaaaa tgaatctatt gtaggggtca tgttggcatc
    12481 aaaaggatat cctgatgcat atgaaaaagg gcataaagta agtggctttg atttaaatga
    12541 aaactatttt gttagtggat taaagaagca aggtgatacc tttgttactt caggtggtag
    12601 agttatactt gccatcggaa aaggtgacaa tgtacaagat gcacagcgag acgcatacaa
    12661 aaaagtatca caaatacaaa gtgaccattt attctatcgt catgacattg cgaataaagc
    12721 actacaactt aaataagtaa atttaaaata ctaagattag ctatgaacga atctataacg
    12781 atagattttt tcatagcttt tttagttgta gagtctagga cattgatttc tgtaccaaat
    12841 ttgtgattat gcatatgtaa tacaaaagag gcgccacaac atgtttggat gaacaaaata
    12901 acatgtttgt ggcacctctt ttgtttagta tggaataaat ggttttcttt ttctatacaa
    12961 tgaatttcta atttagtatc tatacaatta tggataaaat ttaacctaca cgaccaagac
    13021 gaacatcatc tatgcccgtg atgggtaagg tgattgaaca ataatatgcc atagtaataa
    13081 tggcaattaa aactataata aagattatat ctttatatga gaaaggtacg ttgtaatagt
    13141 aagtacgagg accatctcta aatcctttcg actccatcgc aactgataat tgatgtgcct
    13201 ttctaatatt ttggcttaat agaggtataa ttaaatgctt aaatcgcttt aaccctctat
    13261 aatttgccgc gtctatcatc tgatagcgca tttttaaaga tctgcgaagc tgtattaaag
    13321 aactaatcat taaaggtatc atacgaatgg cagccatgaa tgcataagca acttttgatt
    13381 taacctttaa atgttgcatt aaactataaa atatcatgac aacctgagat gtaagtgcga
    13441 ttaagatacc gaaaaatgaa atagcaatgg ttcttaatga tacatgtaaa ccacgaacta
    13501 aactttctgt tgtaatatgg ataaatccga atttcaaaat tgtatggcta ccattcccgt
    13561 ataaaatcat gaacagggaa gagagtaatg caaagccaat acttatagtt ataaaaattg
    13621 ctgtaatttt aaactgagta ccattaaaca tcaataagaa aactaacatt aagatagtga
    13681 tataaagcat aaaatcgaaa ttatgcacaa atataataaa gaaaaatagt ataattccaa
    13741 gaaatagttt cgttataatg ttgacatcat caacaaatga ttgccgaact ttccattgct
    13801 catacattcg tatcaccatc acaatctagt aacgcaccat ctgaaatttt aagtcttctt
    13861 gatggataac gttcaattat ttcatcgtca tgtgtaacca tgacaatact ttgtcccaaa
    13921 ttaattcgct tttggaaaag tttgatcaac tggaatgtat tatggctatc aagtccaaat
    13981 gtcggttcat ctaaaaagat aatatcagct ttagaactta gtgcggtagc tacgctaagg
    14041 cgtcgttttt gaccaataga caactcataa ggatgttgat cttttacatt ttgtaaatct
    14101 aaaagtttta aaagttgtat cgtttcatca tcactttgat ctttagaaag gtgattaaaa
    14161 tgaatgttaa tttcatcata aaccgaattt gttataaatt gtaattctgg gttttgataa
    14221 actaggtaca tgtgttttgc tgcatgttta atttttgtta aacgctgatt ttcaaaataa
    14281 acatcacctt gatatttaat caattgcata attgattcaa gcaaggttgt tttaccacta
    14341 ccatttgccc ctgtaattgt aatccactca cctagaccaa tttctaaatc tgagaatgag
    14401 agcaatgttg atttaccgcg aataatacgt ccatttttaa attgtaataa gtgtgagttt
    14461 gttgttggaa agtcaacacg acttggtgcg aattcccatg cacgtggatg ccacacacca
    14521 tattcactga gtaaatgaac atacttctgt aatatgattt caggacattc atcggcaatg
    14581 atatttccgt tataatccat caaaatgacg cggtcgacat gattccagat gtgtttaact
    14641 ttatgttcaa cgattacaac cgtttgatct tcccaaagtt caattagttt agtccataaa
    14701 tcttctgttg cttgaacatc taacattgct gtcggttcat ctaaaaacaa tgtttttgat
    14761 tgttgaagaa tggtttcaac aattgccaat ttctgtttca tcccgccact taaatctttg
    14821 atatacgttt caggggtaac atttaaattg accatattta aagcattgat aattaacgca
    14881 tccatgtctt cacgtggtaa ttgtctattt tctaaaacga atgcaagttc ttcgtatact
    14941 tttggcatac aaaactggct atcagggtct tggaaaataa cgccacttaa tgggtcaacg
    15001 attagttcat catatttcat aggtaattca attaaattag gaacaatacc acttaataca
    15061 ttcagaagtg tacttttacc gcaaccagaa ggaccgagta aaagtacttt ttctttgtct
    15121 tgaatagtga tatttaaatg atcgaaaatt ttacgttgac cacttggata ttttaatcgt
    15181 aaatcactta cttttaacac tctgaatgct cctcttataa gttgtcgtaa tcttctttag
    15241 cagctggtct aaataatttt gttacgcctg tcttatctaa agcttttact aaaaggtaag
    15301 ataggacgcc ggcgactact gcaccactaa ttaatctaaa tacgatgaat aatgttaagt
    15361 tccaacctgc aacttcattt aaataaccat agaaataatc tatcgggaaa gccgcgattg
    15421 ctgtacaaaa acctgctaac atagctacca taactgaacg tgattgatat ttaaaaattg
    15481 caaagacaag ttcacacgct aaaccttgta taaaagcgta aacgattgtc ggaatatcga
    15541 aacgacccat aatgatagtt tcgccggcac ctgcagcaaa ttcagccagt aaagcaatac
    15601 ctggttttgg aataattaga tagcagacaa tcgctgccat gaaccaaacc ccgtttgtta
    15661 attgttcgag gtgaaggcct gtagcttgca caccattgta aacaaaccac cataaattgt
    15721 aaataactgc gaatactact gaaataagta cggttactag tatttcagat agctttaaac
    15781 cttttgacat ttttacatcc tcctaataaa aaaacgcaca accatccata ggaaagttat
    15841 gcgttcacaa tatatattag taaaacatat gtatagtaac actttcctac gctagttcaa
    15901 gctagatcag gttcaaaggg tttgagggca agcctcatct cagtataaaa cacccctagt
    15961 gtgtgcgatt tatttaatta attatactgt aagacgtttg taaacttatg tcaataggtt
    16021 gtcttcatga aatttcgttt aattcgattt aaaatttata atattagcat tggatttaaa
    16081 ttgaagatgt agtaggaatg ttagtaatta aagatataaa aatatgtgac atgtaataat
    16141 attgagctga taaatgaaga gggatactta tcaatcatac ctctttaaca acagtgaaga
    16201 acccgtgcat aatggcttac gaattatagt ttataaggaa gaagagggat acatgcgccg
    16261 agcacatgca taaaagcccc taacaactaa aagttgtaag gaaggagagg gatacatgcg
    16321 ccgagcacat gcataaaatc ccctaacaac taaaagttgt aaggggattt aaattaattt
    16381 agtgtatctt ggatatcttg ttttgtttga ttaatatctt ctgttttttc ttctttttta
    16441 tcttttaatt tttcttcaac ttctttagct ttttctgctg cttttttatt ttgattttca
    16501 ttagacatga ttaattcctc ccaaattgga taattattta tatataaatc ttacccggtt
    16561 gtactttcgt taaacttttc taagtctata gcactattta ttcatttatc taaagacaac
    16621 aacattagat taatatataa tgattttgag gtgaacataa tgtcttttct taggaaacac
    16681 gccgaaatta tttttagcta tttaatcggt atcgtttcac tcttcactgg tctcattatt
    16741 ttaattaact tgccattaat taaacaatta aatggtggta aaaaagttga tacacatgtt
    16801 cataatgtgt gggaatttct gaatgcattt ttcagtgaaa ttattaaagt aatgagtcga
    16861 tttataggta atttccctat agttagtgca attgtgataa ttatattcgg tattttagtt
    16921 atgttgattg gtcatacatt acttagaact attaagtatg actatgatat ttctatcttt
    16981 ttcttagtta tcggtatcat gtactttatt attactctta tattaatgac tcaagtttat
    17041 ggattctttg cagtgatttt cattattcca tttacaattc atataggata tatcgtctat
    17101 aaagatgaat tgaatcagga aaatgtaaaa aatcatttca tgtggataat tgtgagttat
    17161 ggtataagtt acttaattac acaaattgca ttgtatggca gaattgatgc taatgaaata
    17221 gagtcaattg atatcttaag tgtcaatgct ttctttataa ttatgtggtt acttggtcaa
    17281 atggctattt ggaatttctt gttcttgcgc cgagctttac ctttaacaaa gcaagaatta
    17341 ggtgaagagg agccagaatt atcaagaaca agtaaaggga atgtcacgaa tcaaactaaa
    17401 attcacttga aacaactcca agataagact acagaatatg cacgtaagac aagaagaagt
    17461 gtcgatttag ataaaattag agctaaaaga gataaattca aaaagaaagt taatgatatt
    17521 atcgatattc aagaagacga tattcctgat tggatgagaa aaccgaaatg ggttaaacca
    17581 atgtatgtcg aactattttg tggtgtcgtc atctttttat tcacattttt agaatttaat
    17641 aatcgtaatg cattatttgt atctggtgat tggaaattat cacagacaca atatgttatt
    17701 gaatgggtta cattattaat tctgttattc attattatcg catatatcgc tacaacgtta
    17761 actttccact tgaaaggtaa gttttattat ttacaattat ttatggggag cattttattc
    17821 tttaaattgt taacggaatt tataaatata atgattcatg gactattact ttcagtgttc
    17881 attacgccaa cattactatt aatgttattg gcaatcatca tttcttattc gttacaatta
    17941 cgagagcgac cataattaaa agcattataa aagtactatc tattaaacat tttgatgtgt
    18001 acgctataag ttagatatat ctctaactta cttagataca ggtcaatgaa gtttatggat
    18061 agtacttttt ttgtaactag atttgattga ttcaggtgat gtgaattaag tattgaaaat
    18121 tgtatacaaa gttttagtgc aaataaaata gttgaaaagt tatccatttg taaaatcaag
    18181 aaaactagta aatagttgaa gcgacttatg gaatttgcga aacgatatat agtatttcct
    18241 ttgtagaaat ttaacatata tcattcgaat tactaatttg ttaaaatcaa cagtaagatt
    18301 agaagtagat gatattgaaa tttggcaaac aatttaatct atataaaact acaacgaaac
    18361 acagaaagga agttgtcaga tgaaaatagc aactctgaac aaaggcaaag aaacaaaata
    18421 ttttaatgga tatcctttaa ttgaagaaga ggatatctat tcacaagatc atttaaaaga
    18481 aggagatatt tttcaaattg tgactgataa atcacaatat gtcgcaacgg cttatgttgg
    18541 tcgccaacat aaaggattag gttgggttct aacatacgat aaagctcaac aaatcaacac
    18601 agctttcttt gtaaaattgt ttaatactgc attagcagaa cgtgattatt attttaatat
    18661 agatggaaca aatgctttta gattatttaa tgctgaaggt gatggtgttg ggggattaac
    18721 aatcgacaat tacgatggtc atttgttgat tcaatggtac tcaaaaggta tttataaatt
    18781 taaatatgcc attcttgaag cggttagaaa agtatttgat tataaatcta tttacgaaaa
    18841 agtaagattt aaagacagcg aatatagtgg tggttttgtt gaaggagatg cacctgagtt
    18901 tccaattgtt atcgaagaaa acttcacatt ttataatgta gaccttgaag atggtttgat
    18961 gacaggtatc tttttagatc aaaaagaagt gcgcaagaaa ttaagggatc aatatgccaa
    19021 agaacgccat gttttaaact tatttagtta tacaggtgct ttttctgcaa tagcagcaag
    19081 tgaggcatct tcaacaacaa gtgtagattt ggctaatcgt tctcgtagtt taactgaaga
    19141 aaattttgga ttaaatgcta ttgatcctaa atcccaatat atttatgtca tggacacttt
    19201 tgatttctat aaatatgctg cacgacatgg acatagttat gacacgatcg tgattgatcc
    19261 acctagcttt gcgcgtaaca aaaaacgtac attttcagtg caaaaagatt atgacaaatt
    19321 aattaatggc gccttaaata tcttatcatc tgaaggaaca ttattgttat gtacaaacgc
    19381 aagtgtatat ccattaaagc aatttaaaaa tactattaaa aagacgcttg aagagagtgg
    19441 cgttgattat gaattaactg aagttatggg attaccaaaa gattttaaaa cgcatccaca
    19501 ttataagcca tctaaatatt taaaagctgt ttttgtaaat attagacatt aatttgataa
    19561 aaaggggtat agtaacttat tgagaaaaag aagggtgata atattatggg attcaaaaac
    19621 aatttaacat caaatttaac aaataaaatc ggtaattcag tctttaaaat agaaaatgtt
    19681 gacggaaaag gtgcaatgcc aacgacgatt caagaattga gagaaagacg acaacgtgct
    19741 gaagcaattg taaagagaaa gtctttaatg tcatcaacaa tgagcgttgt tccaattccg
    19801 ggtttagatt ttggtgttga tttaaaatta atgaaagata ttatcgaaga tgttaataaa
    19861 atttatggtt tagatcataa gcaagttaat agccttgggg atgatgtgaa agaaagaatt
    19921 atgtctgcag cagcaattca aggtagtcaa tttattggta aaagaatttc aaatgcattt
    19981 ttaaaaattg taattagaga tgtagctaaa cgtactgctg caaaacaaac aaaatggttt
    20041 cctgttgtag gacaagctgt gtctgcatct attagttact attttatgaa taaaattgga
    20101 aaagatcaca ttcaaaaatg cgaaaatgtt attaaaaatg tcatgtaggt gctataatag
    20161 ttttgcaatt tgcaaatttt actgaaaccg gttttaaacg aattgaattt aaagcatggt
    20221 tttggtaaag ttaatgtata aaactaagtt agtattgtaa taatattgaa gattctaact
    20281 atacgaagga gaaatgtaat tatggaacaa aattcatatg taatcatcga cgagactggt
    20341 attcacgcta gaccagcaac aatgttagta caaacagctt caaaattcga ttctgatatt
    20401 caattagaat ataacggtaa gaaagtaaac ttaaaatcaa tcatgggtgt tatgagcctt
    20461 ggtgttggta aagatgctga aattacaatt tatgctgacg gtagtgatga atctgacgcc
    20521 attcaagcaa tcagtgacgt cttatcaaaa gaaggattga ctaaataatc atgtctaaat
    20581 taattaaagg tattgccgca tctgatggtg tcgcaattgc taaagcttat ttattagttg
    20641 agccagactt aacattcgac aaaaatgaaa aagtcactga tgttgaagga gaagttgcaa
    20701 agttcaatag cgctatcgaa gcttctaaag ttgagttaac taaaattaga aataatgcag
    20761 aggttcaact aggtgctgat aaagctgcta tctttgatgc acatttatta gttttagatg
    20821 accctgaatt aattcaacca atccaagata agattaaaaa tgaaaacgct aatgctgcta
    20881 cagcattaac ggatgtaaca acacaatttg ttacaatttt tgaatctatg gataacgaat
    20941 acatgaaaga acgtgcggct gatattcgcg acgtttctaa acgtgtgtta tcacatattt
    21001 taggtgtaga attaccgaat ccgagtatga ttgatgaaag cgttgttatt gtagggaatg
    21061 acttaacgcc atctgatact gctcaattaa ataaagaatt cgtacaaggt tttgctacaa
    21121 acattggcgg aagaacaagt cactctgcaa ttatgagtcg ttctttagaa attccagcaa
    21181 ttgttggtac aaaatcaatt actcaagaag ttaaacaagg cgacatgatt atcgtagatg
    21241 gattaaatgg tgatgtaatc gttaatccaa ctgaagatga gttaatcgct tatcaagata
    21301 aacgtgagcg ttattttgct gacaagaaag aattacaaaa actacgtgat gctgatactg
    21361 ttacagttga tggtgttcac gcagagcttg ctgcaaatat tggtacacct aatgatttgc
    21421 caggtgttat tgaaaatggt gcacaaggta tcggcttata tagaactgag tttttatata
    21481 tgggtcgtga ccaaatgcct acagaagaag aacaatttga agcttataaa gaagtattag
    21541 aagcaatggg cggtaaacgt gttgttgtac gtactttaga tataggtgga gataaagaat
    21601 tatcatactt aaacttgcct gaagaaatga atccattctt aggttaccgt gcgattcgtt
    21661 tatgccttgc gcaacaagat attttcagac cacagctacg tgcattatta cgtgcatcag
    21721 tttatggtaa gttaaatatc atgttcccaa tggttgcaac aattaacgaa tttagagaag
    21781 ctaaagctat attattagaa gaaaaagaaa accttaaaaa tgaaggtcat gacatttcgg
    21841 atgatataga attaggaatc atggtagaga tacctgcaac agcagcatta gctgatgtct
    21901 ttgctaaaga agtagatttc ttcagtatcg gtacaaatga tttaattcaa tacacattag
    21961 ctgctgaccg tatgtcagag cgtgtatcat atctatacca accatataac ccttcaatct
    22021 tacgtttagt taaacaagtt attgaagcgt cacataaaga aggtaaatgg acaggtatgt
    22081 gtggtgaaat ggctggagat gaaacagcta ttccattatt gcttggttta ggtttagatg
    22141 agttctctat gagtgcaacg tctattctga aagcaagaag acaaattaat ggtttaagta
    22201 aaaatgaaat gactgaactt gctaaccgtg cagtcgactg tgcaacgcaa gaagaagtta
    22261 ttgaattagt taacaactac gtaaaataat taattctttc gaaagacaga attataaata
    22321 aacccgaagt attcattgta agtaattatg aatacttcgg gttctttttt atgatttatg
    22381 ttaatttcaa tggaatgttg ttg
//
LOCUS       DAKTIO010000024        21869 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.024, whole genome shotgun sequence.
ACCESSION   DAKTIO010000024 DAKTIO010000000
VERSION     DAKTIO010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21869
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.024"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            3..1607
                     /locus_tag="O0293_000706"
     CDS             3..1607
                     /locus_tag="O0293_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCY0279604.1"
                     /translation="MLNKERIDLFNYLQKHIYSRDDVYFDEQKIEDCIKFIEKWYFPT
                     LPFQRFIIANIFLIDKNTDEAFFTEFAIFMGRGGGKNGLISAISDFLSTPLHGVKEYH
                     ISIVANSEDQAKTSFDEIRTVLMDNKRNKTGKTPKAPYEVSKAKIINRATKSVIRYNT
                     SNTKTKDGGREGCVIFDEIHYFFGPEMVNVKRGGLGKKKNRRTFYISTDGFVREGYID
                     AMKHKIASVLSGKVKNSRLFAFYCKLDDPKEVDDRQTWEKANPMLHKPLSEYAKTLLS
                     TIEEEYNDLPFNRSNKPEFMTKRMNLPEVDLEKVIAPWKEILATNREIPNLDNQMCIG
                     GLDFANIRDFASVGLLFRKNDDYIWLGHSFVRQGFLDDVKLEPPIKEWEKMGLLTIVD
                     DDVIEIEYIVDWFLKAREKYGLEKVIADNYRTDIVRRAFEDAGIKLEVLRNPKAIHGL
                     LAPRIDTMFAKHNVIYGDNPLMRWFTNNVAVKVKPDGNKEYIKKDENRRKTDGFMAFV
                     HALYRADDIVDKDMSKALDALMSIDF"
     gene            1623..2810
                     /locus_tag="O0293_000707"
     CDS             1623..2810
                     /locus_tag="O0293_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCY0279605.1"
                     /translation="MSILEKIFKTRKDITYMLDLDMIEDLSQQAYVKRLAIDSCIEFV
                     ARAVAQSHFKVLEGNRIQKNDVYYKLNIKPNTDLSSDSFWQQVIYKLIYDNEVLIVVS
                     DSKELLIADSFYREEYALYDDIFKDVTVKDYTYQRTFTMQEVIYLKYNNNKVTHFVES
                     LFEDYGKIFGRMIGAQLKNYQIRGILKSASSAYDEKNIEKLQAFTNKLFNTFNKNQLA
                     IAPLIEGFDYEELSNGGKNSNMPFSELSELMRDAIKNVALMIGIPPGLIYGETADLEK
                     NTLVFEKFCLTPLLKKIQNELNAKLITQSMYLKDTRIEIVGVNKKDPLQYAEAIDKLV
                     SSGSFTRNEVRIMLGEEPSDNPELDEYLITKNYEKANSGENDEKEKDENTLKGGDEDE
                     SGD"
     gene            2794..3531
                     /locus_tag="O0293_000708"
     CDS             2794..3531
                     /locus_tag="O0293_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCY0279606.1"
                     /translation="MKAEIKGVIVSNEDKWVYEMLGMDSTCPKDVLTQLEFSDEDVDI
                     IINSNGGNLVAGSEIYTHLRAHKGKVNVRITAIAASAASLIAMAGDHIEMSPVARMMI
                     HNPSSIAQGEAKDLNHAAETLEHVGQIMAEAYAVRAGKNKQELVEMMARETWLNADEA
                     IEQGFADSKMFENDNMQIVASNTQVLSKDVLNRVTALVSKTPEVNIDIDAIANKVIEK
                     INMKEKESEIDVADSKVSANGFSRFLF"
     gene            3555..4700
                     /locus_tag="O0293_000709"
     CDS             3555..4700
                     /locus_tag="O0293_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCY0279607.1"
                     /translation="MTINLSETFANAKNEFINAVNNGEPQERQNELYGDMINQLFEET
                     KLQAKAEAERVSSLPKSAQSLSANQRSFFMDINKNVNYKEEKLLPEETIDRIFEDLTT
                     NHPLLADLGIKNAGLRLKFLKSETSGVAVWGKIYGEIKGQLDAAFSEETAIQNKLTAF
                     VVLPKDLNDFGPAWIERFVRVQIEEAFAVALETAFLKGTGKDQPIGLNRQVQKGVSVT
                     EGAYPEKEEQGTLTFANPRATVNELTQVFKYHSTNEKGKSVAVKGNVTMVVNPSDAFE
                     VQAQYTHLNANGVYVTALPFNLNVIESTVQEAGKVLTYVKGLYDGYLAGGINVQKFKE
                     TLALDDMDLYTAKQFAYGKAKDNKVAAVWKLDLKGHKPALEGTEETL"
     gene            4720..5004
                     /locus_tag="O0293_000710"
     CDS             4720..5004
                     /locus_tag="O0293_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279608.1"
                     /translation="MVKFKVVRAFKDIEHNQHKYKVGELYPAEGYNNPRVELLTNQIK
                     NKYDKVYIVPLDKLTKQELLELCESLQKKASSSMVKSEIVDLLNGEDNDD"
     gene            4994..5278
                     /locus_tag="O0293_000711"
     CDS             4994..5278
                     /locus_tag="O0293_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HCY0279609.1"
                     /translation="MTIDDLLVKFKSLEKIDHNSEDEYLKQLLKMSYERIKNQCGVFE
                     LENLIGQELILIRARYAYQDLLEHFNDNYRPEIIDFSLSLMEVSEDEESV"
     gene            5262..5624
                     /locus_tag="O0293_000712"
     CDS             5262..5624
                     /locus_tag="O0293_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HCY0279610.1"
                     /translation="MKKVFKKPRITTKRLNTRVHFYKYTENNGPEAGEKEEKLLYSCW
                     ASIDGVWLRELEQAISNGTQNDIKLYIRDPQGDYLPSEEHYLEIESRYFKNRLNIKQV
                     SPDLDNKDFIMIRGGYSS"
     gene            5621..6025
                     /locus_tag="O0293_000713"
     CDS             5621..6025
                     /locus_tag="O0293_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCY0279611.1"
                     /translation="MSVKVIGDKALERELENRFGTQAMLKVQDRALIAGAKVIVEEVK
                     KQLKPSKDTGALINEVSFSKPEWINGKRTITVHWRGSKDRYKIVHLIENGHVQKGTGK
                     FIKPKAMGGVNRAIRQGQNKYFETLKRELKKL"
     gene            6022..6426
                     /locus_tag="O0293_000714"
     CDS             6022..6426
                     /locus_tag="O0293_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279612.1"
                     /translation="MIDILYKVHEVISQDRIIREHVNINNIKFNKYPNVKDTDVPFIV
                     IDDIDDPIPTTYTDGDERAYSYIVQIDVFVKFNEKYNARIIRNKISNRIQKLLWSELK
                     MGNVSNGKPEYIEEFKTYRSTRVYEGIFYEEE"
     gene            6430..7074
                     /locus_tag="O0293_000715"
     CDS             6430..7074
                     /locus_tag="O0293_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCY0279613.1"
                     /translation="MVVKQASAPKAYFNITGLGFAKLTKEGAKLEYSDITKTRGLQKI
                     GVETGGDLKTAYADGGPIESGNTDGEGKISLQMHAFPKEIRKIVFNEEYDEYGVYKET
                     QGKQNNYVAIWFRQERRDGTFRTVLLPKVMFTNPKIDGETAEKDWDFSSEEVEGEALF
                     PLIDNKKSVRKYIFDSANMTNHSGNGEKGEEAFLKKILGEEYTGNVTEDNGETL"
     gene            7101..7340
                     /locus_tag="O0293_000716"
     CDS             7101..7340
                     /locus_tag="O0293_000716"
                     /inference="COORDINATES: protein motif:HMM:NF014427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCY0279614.1"
                     /translation="MKSVNIPDSFKTLKVGNTYDLNVVVEPSNQSKLLKYTTDQTNIV
                     SINSDGQVTAEAQGIATVKAYVGNMSDTITINVEA"
     gene            7390..7740
                     /locus_tag="O0293_000717"
     CDS             7390..7740
                     /locus_tag="O0293_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279615.1"
                     /translation="MAKLKRNIIQLVEDPKANEIKLQTYLTPHFISFEIVYEAMDLID
                     DIEDENSTMKPREIADRLMDMVVKIYDNQFAVKDLKERMHAPDGMNALREQVVFITQG
                     QQTEETRNFIQNMK"
     gene            7779..7928
                     /locus_tag="O0293_000718"
     CDS             7779..7928
                     /locus_tag="O0293_000718"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279616.1"
                     /translation="MDTLMMDLIENGKDANEVLKMPFHYVLSIYQNKNNDISEEKAEA
                     LIDAF"
     gene            7985..12514
                     /locus_tag="O0293_000719"
     CDS             7985..12514
                     /locus_tag="O0293_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001799555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCY0279617.1"
                     /translation="MGERIKGLSIGLDLDAANLNRSFAEIKRNFKTLNSDLKLTGNNF
                     KYTEKSTDSYKQRIKELDGTITGYKKNVDDLAKQYDKVSQEQGENSAGAQKLRQEYNK
                     QANELNYLERELQKTSAEFEEFKKAQVEAQRMAESGWGKTSKVFESMGPKLTKMGDGL
                     KSIGKGMMIGVTAPVLGIAAASGKAFAEVDKGLDTVTQATGATGSELKKLQNSFKDVY
                     GNFPADAETVGGVLGEVNTRLGFTGKELENATESFLKFSHITGSDGVQAVQLITRAMG
                     DAGIEASEYQSVLDMVAKAAQASGISVDTLADSITKYGAPMRAMGFEMKESIALFSQW
                     EKSGVNTEIAFSGLKKAISNWGKAGKNPREEFKKTLAEIEKTPDIASATSLAIEAFGA
                     KAGPDLADAIKGGRFSYQEFLKTIEDSQGTVNQTFKDSESGSERFKVAMNKLKLVGAD
                     VWASIESAFAPVMEELIKKLSVAVDWFSNLSDGSKRSIVIFGGIAAAIGPVVFGLGAF
                     ISTIGNAVTVLAPLLAGIAKADGLISFLSTKVPILGTVFTALTGPIGIVLGVLAGLAV
                     AFTIAYKKSETFRNFVNDAIESVKQTFSNFIQFIQPFIDSVKNVFKQAVSAIVDFAKD
                     IWSQINGFFNENGISIVQALQNICNFIKVIFEFILNFVIKPIMFAIWQVMQFIWPAVK
                     ALIVSTWENIKGVIQGTLNIILGLIKFFSSLFTGDWRGVWDAIIMILKGVVQLIWNLI
                     QLWFVGKILGVVRYFGGLLKGLITGIWDVIKSIFSKSLSAIWNATKSIFGFLFNSVKS
                     IFTNMKNWLSNTWSSIRTNTIGKAQSLFSGVKSKFTSLWNATKDIFSNLRNWMANIWN
                     SIKDNTVGIASRLWSKVRGIFTNMRDGLQSIISKIKSHIGGMVDAIKKGLNKLIEGLN
                     WVGGKLGMDKIPKLHTGTEHTHTTTRLVKNGKIARDTFATVGDKGRGNGPNGFRNEII
                     EFPNGKRVITPNTDTTAYLPKGSKVYNGTQTYSMLNGTLPRFSLGTMWKDIKSGASSA
                     FNWTKDQIGKGTKWLGDKVGDVLDFMENPGKLLNYILEAFGIDFNSLTKGMGIAGDIT
                     KAAWSKIKKSATDWIKENLEAMGGGDLVGGILDPDKINYHYGRTAAYTAATGRPFHEG
                     VDFPFVYQEVRTPMGGRLTRMPFMSGGYGNYVKITSGVIDMLFAHLKNFSKSPPSGTM
                     VKPGDVVGLTGNTGFSTGPHLHFEMRRNGRHFDPEPYLRNAKKKGRLSIGGGGATSGS
                     GATYASRVIRQAQSILGGRYKGKWIHDQMMRVAKRESNYQSNAVNNWDINAQRGDPSR
                     GLFQIIGSTFRANAKRGYTNFNNPVHQGISAMQYIVRRYGWDGFKRAGDYAYATGGKV
                     FDGWYNLGEDGHPEWIIPTDPARRNDAMKMLHYAAAEVRGRKASKNKRPSQLSSVNGF
                     DDPSLLLKMIEQQQQQIALLLKIAQSNDVIADKDYQPIIDEYAFDKKVNASIEKRERQ
                     ESTKVKFRKGGIAIQ"
     gene            12511..13995
                     /locus_tag="O0293_000720"
     CDS             12511..13995
                     /locus_tag="O0293_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HCY0279618.1"
                     /translation="MIDTIKVNNKTIPWLYVERGFEIPSFNYVLKTENVDGRSGSIYK
                     GRRLESYSFDIPLVVRNDYLSHNGFKTHDDVLNELVKFFNYEEQVKLQFKSKDWYWNA
                     YFEGPIKLHKEFAIPVKFTIKVVLTDPYKYSVTGYKNTAISDQVSVVNSGTADTPLIV
                     EARAIKPSSYFMITKNDEDYFMVGDDEVTKEVKDYMPPVYHSEFRDFKGWTKMITEDI
                     PSNDLGGKVGGDFVISNLGEGYKATNFSDAKGWVGAGTKRGLPKAMTDFQITYKCIVE
                     QKGKGAGRTAQHIYDSDGKLLASIGYENKYHDRKIGHIVVTLYNQKGDPKKIYDYQNK
                     PIMYNLDRIVVYMRLRRVGNKFSIKTWKFDHIKDPDRRKPIDMDEKEWIDGGKFYQRP
                     ASIIAIYSAKYNGYKWMEMNGLGSFNTEILPKPKGARDVIIQKGDLVKIDMQAKSVVI
                     NEEPMLSEKSFGSNYFNVDSGYSELIIQPENVFDTTVKWQDRYL"
     gene            14011..17796
                     /locus_tag="O0293_000721"
     CDS             14011..17796
                     /locus_tag="O0293_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279619.1"
                     /translation="MIHVLDFNDKIIDFLSTDDPSLVRAIHKRNVNDNSEMLELLISS
                     ERAEKFRERHRVIIRDSNKQWREFIINWVQDTMDGYTEIECIASYLADITTAKPYAPG
                     KFEKKTTSEALKDVLSDTGWEVSEQTEYDGLRTTSWTSYQTRYEVLKQLCTTYKMVLD
                     FYIELSSNTVKGRYVVLKKKNSLFKGKEIEYGKDLVGLTRKIDMSEIKTALIAVGPEN
                     DKGKRLELVVTDDEAQSQFNLPMRYIWGIYEPQSDDQNMNETRLSSLAKTELNKRKSA
                     VMSYEITSTDLEVTYPHEIISIGDTVRVKHRDFNPPLYVEAEVIAEEYNIISENSTYT
                     FGQPKEFKESELREEFNKRLNLIHQKLNDNISNINTIVKDVVDGELEYFERKIHKSDT
                     PPENPVNDTLWYDTSNPDVAVLRRYWNGRWIEATPNDVEKLGGITREKALFSELNNIF
                     INLSIQHASLLSEATELLNSEYLVDNDLKADLQASLDAVIDVYNQIKNNLESMTPETA
                     TIGRLVDTKTLFLEYRKKLQDVYTDVEDVKIAISDRFKLLQSQYTDEKYKEALEIIAT
                     KFGLTVNEDLQLVGEPNVVKSAIEAARESTKEQLRDYVKTSDYKTDKDGIVERLDTAE
                     AERTTLKGEIKDKVTLNEYRNGLEEQKQYTDDQLSDLSNNPEIKASIEQANQEAQEAL
                     KSYIDAQDNLKEKESQAYADGKISEEEQRAIQDAQAKLEEAKQNAELKARNAEKKANA
                     YTDNKVKESTDAQRRTLTRYGSQIIQNGKEIKLRTTKEEFNATNRTLSNILNEIVQNV
                     TDGTTIRYDDNGVAQALNVGPRGIRLNADKIDINGNREINLLIQNMRDKVDKTDIVNS
                     LNLSREGLDINVNRIGIKGGNNNRYVQIQNDSIELGGIVQRTWKGKRSTDDIFTRLKD
                     GHLRFRNNTAGGSLYMSHFGISTYIDGEGEDGGSSGTIQWWDKTYSDSGMNGITINSY
                     GGVVALTSDNNRVVLESYASSNIKSKQAPVYLYPNTDKVPGLNRFAFTLSNADNAYSS
                     DGYIMFGSDENYDYGAGIRFSKERNKGLVQIVNGRYATGGDTTIEAGYGKFNMLKRRD
                     GNRYIHIQSTDLLSVGSDDAGDRIASNSIYRRTYSAAANLHITSAGTIGRSTSARKYK
                     LSIENQYNDRDEQLEHSKAILNLPIRTWFDKAESEILARELREDRKLSEDTYKLDRYV
                     GLIAEEVENLGLKEFVTYDDKGEIEGIAYDRLWIHLIPVIKEQQLRIKKLEESKNAG"
     gene            17786..17938
                     /locus_tag="O0293_000722"
     CDS             17786..17938
                     /locus_tag="O0293_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279620.1"
                     /translation="MQDNKQGLQANPEYTIHYLSQEIMRLTQENAMLKAYIQENKENQ
                     QCAEEE"
     gene            17985..18272
                     /locus_tag="O0293_000723"
     CDS             17985..18272
                     /locus_tag="O0293_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279621.1"
                     /translation="MAKEIINNTERFILVQIDKEGTERVVYQDFTGSFTTSEMVNHAQ
                     DFKSEENAKKIAETLNLLYQLTNKKQRVKVVKEVVERSDLSPEVTVNTETV"
     gene            18330..18626
                     /locus_tag="O0293_000724"
     CDS             18330..18626
                     /locus_tag="O0293_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HCY0279622.1"
                     /translation="MGWFKKHEHEWRIRRLEENDKTMLSTLNEIKLGQKTQEQVNIKL
                     DKTLDAIQKEREIDEKNKKENDKNIRDMKMWVLGLVGTIFGSLIIALLRMLMGI"
     gene            18776..18952
                     /locus_tag="O0293_000725"
     CDS             18776..18952
                     /locus_tag="O0293_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HCY0279623.1"
                     /translation="MDRWWLSEYKEVVPMVALLKSLERRRLMITISTMLQFGLFLIAL
                     IGLVIKLIELSNKK"
     gene            complement(19005..19112)
                     /locus_tag="O0293_000726"
     CDS             complement(19005..19112)
                     /locus_tag="O0293_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279624.1"
                     /translation="MIFILWCVNLYIEKGQHAQTCYPNEPVKKTVAILD"
     gene            19164..19418
                     /locus_tag="O0293_000727"
     CDS             19164..19418
                     /locus_tag="O0293_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCY0279625.1"
                     /translation="MINWKIRMKQKSFWVAILSAIFLFAQNIAKAIGYDIQVYTEQLT
                     DGLNAILGFLVLTGVIQDPTTKGIGDSHQALEYEEPRRKY"
     gene            19430..20185
                     /locus_tag="O0293_000728"
     CDS             19430..20185
                     /locus_tag="O0293_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0279626.1"
                     /translation="MKTYSEARARLRWYQGRYIDFDGWYGYQCADLAVDYIYWLLEIR
                     MWGNAKDAINNDFKNMATVYENTPSFVPQIGDVAVFTKGIYKQYGHIGLVFNGGNTNQ
                     FLILEQNYDGNANTPAKLRWDNYYGCTHFIRPKYKSEGLMNKITNKVKPPAQKAVGKS
                     ASKITVGSKAPYNLKWSKGAYFNAKIDGLGATSATRYGDNRTNYRFDVGQAVYAPGTL
                     IYVFEIIDGWCRIYWNNHNEWIWHERLIVKEVF"
     gene            20376..20867
                     /gene="sak"
                     /locus_tag="O0293_000729"
     CDS             20376..20867
                     /gene="sak"
                     /locus_tag="O0293_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylokinase"
                     /protein_id="HCY0279627.1"
                     /translation="MLKRGLLFLTVLLLLFSFSSITNEVSASSSFDKGKYKKGDDASY
                     FEPTGPYLMVNVTGVDGKGNELLSPHYVEFPIKPGTTLTKEKIEYYVEWALDATAYKE
                     FRVVELDPSAKIEVTYYDKNKKKEETKSFPITEKGFVVPDLSEHIKNPGFNLITKVII
                     EKK"
     gene            21517..21852
                     /locus_tag="O0293_000730"
                     /pseudo
     CDS             21517..21852
                     /locus_tag="O0293_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
BASE COUNT         8066 a         3003 c         4476 g         6324 t
ORIGIN      
        1 taattttaaa taaagaaaga attgatctct ttaattatct acaaaaacat atatattcac
       61 gagatgatgt atattttgat gaacagaaaa tcgaggattg tatcaaattt attgaaaaat
      121 ggtattttcc aacattacca tttcaaaggt ttatcatagc taatatattt cttatagata
      181 aaaatacaga tgaagctttc tttacagaat ttgctatttt catgggacgt ggaggcggga
      241 aaaacggtct aataagtgct attagtgatt ttctttctac gcccttacac ggagttaaag
      301 aatatcacat ctccattgtt gctaatagtg aagatcaagc aaaaacatcg tttgatgaaa
      361 tcagaaccgt tttaatggat aacaaacgaa ataagacggg taaaacgcca aaagctcctt
      421 atgaagttag taaagcaaaa ataataaacc gtgcaactaa atcggttatt cgatataaca
      481 catcaaacac aaaaaccaaa gacggtggac gtgaggggtg tgttattttt gatgaaattc
      541 attatttctt tggtcctgaa atggtaaacg tcaaacgtgg tggattaggt aaaaagaaaa
      601 atagaagaac gttttatata agtactgatg gttttgttag agagggttat atcgatgcaa
      661 tgaagcacaa aattgcaagt gtattaagtg gcaaggttaa aaatagtaga ttgtttgctt
      721 tttattgtaa gttagacgat ccaaaagaag ttgatgacag acagacgtgg gaaaaggcga
      781 acccaatgtt acataaaccg ttatcagaat acgctaaaac actgctaagt acgattgaag
      841 aagaatataa cgatttacca ttcaaccgtt caaataagcc cgaattcatg actaagcgaa
      901 tgaatttgcc tgaagttgac cttgaaaaag taatagcacc atggaaagaa atactagcga
      961 ctaatagaga gataccaaat ttagataatc aaatgtgtat tggtggttta gactttgcaa
     1021 acattcgaga ttttgcaagt gtagggctat tattccgaaa aaacgatgat tacatttggt
     1081 taggacattc gtttgtaaga caagggtttt tggatgatgt caaattagaa cctcctatta
     1141 aagaatggga aaaaatggga ttattgacca ttgtcgatga tgatgtcatt gaaattgaat
     1201 atatagttga ttggttttta aaggctagag aaaaatatgg gcttgaaaaa gtcatagctg
     1261 ataattatag aactgatatt gtaagacgtg cgtttgagga tgctggcata aaacttgaag
     1321 tacttagaaa tccaaaagca atacatggat tacttgcacc acgtatcgat acaatgtttg
     1381 cgaaacataa cgtaatatat ggagacaatc ctttgatgcg ttggtttact aataatgttg
     1441 cagtaaaggt taaacccgat ggtaataaag aatatattaa aaaagatgaa aatagaagaa
     1501 aaaccgatgg gttcatggct tttgttcacg cattatatag agcagacgat atagtagaca
     1561 aagacatgtc taaagcgctt gatgcattaa tgagtataga tttctaatag aggaggtgag
     1621 acatgagtat tctagaaaag atatttaaaa ctaggaaaga tataacatat atgcttgatt
     1681 tagatatgat agaagatcta tcacaacaag cgtatgtgaa acgtttagcg attgatagtt
     1741 gtattgaatt tgttgcgcga gctgtcgctc aaagtcattt taaagtattg gaaggtaata
     1801 gaattcaaaa gaatgatgtt tactacaagt taaatataaa accaaatact gacttatcaa
     1861 gcgatagttt ttggcaacaa gttatatata aactaattta tgataacgag gttttaatcg
     1921 tagtaagtga cagcaaagaa ttacttatcg cagatagctt ttacagagaa gagtacgctt
     1981 tgtatgatga tatattcaaa gatgtaacgg ttaaagatta tacttatcaa cgtactttca
     2041 caatgcaaga ggtcatatat ttaaagtaca acaacaataa agtgacacac tttgtagaaa
     2101 gtctattcga agattacggg aaaatattcg gaagaatgat aggtgcacaa ttaaaaaact
     2161 atcaaataag agggattttg aaatctgcct ctagcgcata tgacgaaaag aatatagaaa
     2221 aattacaagc gttcacaaat aaattattca atacttttaa taaaaatcaa ctagcaatcg
     2281 cgcctttgat agaaggtttt gattatgagg aattatctaa tggtggtaag aatagtaaca
     2341 tgcctttttc tgaattgagt gagctaatga gagatgcaat aaaaaatgtt gcgttgatga
     2401 ttggtatacc tccaggtttg atttacggag aaacagctga tttggaaaag aacacgcttg
     2461 tatttgagaa gttctgttta acacctttat taaaaaagat tcagaacgaa ttaaacgcga
     2521 aactcataac acaaagcatg tatttgaaag atacaagaat agaaattgtc ggtgtgaata
     2581 aaaaagaccc acttcaatat gctgaagcaa ttgacaaact tgtaagttct ggttcattta
     2641 caaggaatga ggtgcggatt atgttaggtg aagaaccatc agacaatcct gaattagacg
     2701 aatacctgat tactaaaaac tacgaaaaag ctaacagtgg tgaaaatgat gaaaaagaaa
     2761 aagatgaaaa cactttgaaa ggtggtgatg aagatgaaag cggagattaa aggcgtcatc
     2821 gtttccaacg aagataaatg ggtttacgaa atgcttggta tggattcgac ttgtcctaaa
     2881 gatgttttaa cacaactaga atttagtgat gaagatgttg atattataat taactcaaat
     2941 ggtggtaacc tagtagctgg tagtgaaata tatacacatt taagagctca taaaggcaaa
     3001 gtgaatgttc gcatcacagc aatagcagca agtgcggcat cgcttatcgc aatggctggt
     3061 gaccacatcg aaatgagtcc ggttgctaga atgatgattc acaatccttc aagtattgcg
     3121 caaggagaag cgaaagatct aaatcatgct gcagaaacat tagaacatgt tggtcaaata
     3181 atggctgagg catatgcggt tagagctggt aaaaacaaac aagaacttgt agaaatgatg
     3241 gctagggaaa cgtggctaaa tgctgatgaa gccattgaac aaggttttgc ggatagtaaa
     3301 atgtttgaaa acgacaatat gcaaattgta gcaagcaata cacaagtgtt atcgaaagat
     3361 gtattaaatc gtgtaacagc tttggtaagt aaaacgccag aggttaacat tgatattgac
     3421 gcaatagcaa ataaagtaat tgaaaaaata aatatgaaag aaaaggaatc agaaatcgat
     3481 gttgcagata gtaaagtatc agcaaatgga ttttcaagat tcctttttta atacaaaaaa
     3541 taggaggtca taaaatgact ataaatttat cggaaacatt cgcaaatgcg aaaaacgaat
     3601 ttattaatgc agtaaacaac ggtgaaccgc aagaaagaca aaatgaattg tacggtgaca
     3661 tgattaacca actatttgaa gaaactaaat tacaagcaaa agcagaagct gaaagagttt
     3721 ctagtttacc taaatcagca caatctttga gtgcaaacca aagaagtttc ttcatggata
     3781 tcaataaaaa cgttaactat aaagaagaaa aacttttgcc agaagaaaca attgatagaa
     3841 tttttgaaga tttgacgacg aatcatccgt tattagctga tttaggtatt aaaaacgctg
     3901 gtttgcgttt gaagttctta aaatctgaaa cttctggcgt agccgtttgg ggtaaaatct
     3961 atggtgaaat taaaggtcaa ttagatgctg cgttcagtga agaaacagca attcaaaata
     4021 aattgacagc gtttgttgtt ttaccaaaag atttaaatga ttttggtcct gcgtggattg
     4081 aaagatttgt tcgtgttcaa atcgaagaag catttgcagt ggcgcttgaa actgcgttct
     4141 taaaaggtac tggtaaagac caaccaatcg gcttaaaccg tcaagtacaa aaaggtgtat
     4201 cggtaactga gggtgcttat ccagagaaag aagaacaagg tacgcttaca tttgctaatc
     4261 cgcgcgctac agttaatgaa ttgacgcaag tgtttaaata ccactcaact aacgagaaag
     4321 gtaaatcagt agcggttaaa ggtaatgtaa caatggttgt taatccgtcc gatgcttttg
     4381 aggttcaagc acagtataca catttaaatg caaatggcgt atatgttact gctttaccat
     4441 ttaatttgaa tgttatcgag tctacagtcc aagaagcagg taaggtttta acgtacgtta
     4501 aaggtttata tgatggttat ttagctggtg gtattaatgt tcagaaattt aaagaaacac
     4561 ttgcgttaga tgatatggat ttatacactg caaaacaatt tgcttacggc aaagcgaaag
     4621 ataataaagt tgctgctgtt tggaaattag atttaaaagg acataagcca gctttagaag
     4681 gtaccgaaga aacactataa aattttatga ggtgataaaa tggtgaaatt taaagttgtt
     4741 agagctttta aagacataga gcacaatcaa cacaagtaca aagtagggga gttgtatcca
     4801 gctgaagggt ataacaatcc tcgtgttgaa ttgttgacaa atcaaatcaa aaataagtac
     4861 gacaaagttt atatcgtacc tttagataag ctgacaaaac aagaattatt agaactatgc
     4921 gaatcattac aaaaaaaagc gtctagttca atggttaaaa gtgaaatcgt cgacttattg
     4981 aatggtgaag acaatgacga ttgatgattt gcttgtcaaa tttaaatcac ttgaaaagat
     5041 tgaccataat tcagaggatg agtacttaaa gcagttgtta aaaatgtcgt acgagcgtat
     5101 aaaaaatcag tgcggagttt ttgaattaga gaatttaata ggtcaagaat tgatacttat
     5161 acgcgctaga tatgcttatc aagatttatt agaacacttc aacgataatt acagacctga
     5221 aataatagat ttttcgttat ctctaatgga ggtatcagaa gatgaagaaa gtgtttaaaa
     5281 aacctagaat tacaactaaa cgtttaaata ctcgtgttca tttttataag tatactgaaa
     5341 ataatggtcc agaagctgga gaaaaagaag aaaaattatt atatagctgt tgggcgagta
     5401 ttgatggtgt ctggttacgt gaattagaac aagctatctc aaacggaacc caaaatgaca
     5461 ttaaattgta tattcgtgat ccgcaaggtg attatttacc cagtgaagaa cattatcttg
     5521 aaattgaatc aagatatttc aaaaatcgtt tgaatataaa gcaagtatca ccagatttgg
     5581 ataataaaga ctttattatg attcgtggag gatatagttc atgagtgtga aagtgatagg
     5641 tgataaagca ttagaaagag aattagaaaa tcgtttcgga actcaagcga tgttaaaagt
     5701 tcaagatagg gcgctaattg ctggtgctaa agtaattgtt gaagaagtaa aaaaacaact
     5761 aaagccatca aaagatacgg gcgcattaat taatgaggta agttttagta aacctgaatg
     5821 gataaacgga aaacgtacaa ttactgttca ttggcgaggt tctaaagacc gttataaaat
     5881 cgtacattta attgaaaatg gacacgttca aaaagggaca ggtaaattta tcaaacctaa
     5941 agctatgggt ggcgttaata gagcaataag acaagggcaa aataagtatt ttgagacgct
     6001 aaaaagggag ttaaaaaaat tgtgattgat attttgtaca aagttcatga agtgattagt
     6061 caagacagaa ttattagaga gcacgtaaat atcaataata ttaagttcaa taaataccca
     6121 aacgtaaaag atacggatgt acctttcata gttattgacg atatagacga cccaatacct
     6181 acaacttata ctgacggaga tgagcgcgca tatagttaca ttgtacaaat agatgtattt
     6241 gtgaagttta acgaaaagta taacgcgaga atcataagaa ataagatatc taatcgtatt
     6301 caaaagttat tatggtctga actgaaaatg ggaaatgtat ccaacggaaa acctgaatat
     6361 atagaagaat ttaaaacata tagaagcact cgtgtctacg agggcatttt ttacgaggag
     6421 gaataataaa tggtagtaaa acaagcaagt gcgccgaaag cgtattttaa cattactggt
     6481 ttaggtttcg ctaaattaac aaaagaaggc gcaaagctag aatatagcga tattacaaaa
     6541 actagaggat tacaaaaaat cggtgttgaa actggaggag acttgaaaac agcatatgct
     6601 gatggtggtc caatcgaatc agggaacaca gacggagaag gtaaaatttc gttacaaatg
     6661 catgctttcc ctaaagagat tcgcaaaata gtattcaatg aagagtatga tgaatacggt
     6721 gtttataaag aaactcaagg taaacaaaac aactatgtag caatttggtt cagacaagag
     6781 cgtcgagacg gcacatttag aacggtttta ttacctaaag tcatgtttac aaatcctaaa
     6841 atcgatggcg aaacggctga gaaagattgg gatttctcaa gcgaagaggt tgaaggtgag
     6901 gcacttttcc ctttaattga taataaaaag tcagtacgta aatatatctt tgattcagca
     6961 aacatgacaa atcatagtgg aaatggtgaa aaaggcgaag aggctttctt aaagaaaatt
     7021 ttaggcgaag aatatactgg aaacgtgaca gaggataacg gcgaaacttt gtaacgcaac
     7081 cggcttcatc ggaaactgag ataaagtcgg ttaatatacc agatagtttt aaaacactaa
     7141 aagttggcaa cacatatgat ttaaatgttg tagtagagcc atctaatcaa agtaagttat
     7201 tgaaatacac aacagatcaa acgaatattg tatcaatcaa tagtgatggt caagtcactg
     7261 cggaagcaca aggtattgcc acggttaaag catatgttgg taatatgagt gacactataa
     7321 caataaatgt agaagcataa gaggggacaa cccctctatt ttatttgaaa ataaggagag
     7381 tattataaaa tggcaaaatt aaaacgtaac attattcaat tagtagaaga tccaaaagca
     7441 aatgaaatta aattacaaac gtacttaaca ccacacttca tttcatttga aattgtatac
     7501 gaagcaatgg atttaatcga tgatattgag gacgaaaata gcacgatgaa gccaagagaa
     7561 atcgctgaca gattgatgga tatggttgta aaaatttacg ataaccaatt cgcagttaaa
     7621 gacctaaaag aacgtatgca tgcacctgat ggaatgaatg cacttcgtga acaagtggtt
     7681 ttcattacgc aaggtcaaca aactgaggaa actagaaatt ttatccagaa catgaaataa
     7741 agcctgaaga tttaacatat aaagcaatgt tgaaaaatat ggatactctc atgatggact
     7801 taattgaaaa tggtaaagac gctaacgaag ttttaaaaat gccatttcat tatgtgcttt
     7861 ccatatatca aaataaaaac aatgacattt ctgaagaaaa agcagaggct ttaattgatg
     7921 cattttaacc ttaaccgttt ggttagggtt atttttttga acttttttag aaaggaggta
     7981 aaaaatggga gaaagaataa aaggtttatc tataggtttg gatttagatg cagcaaattt
     8041 aaatagatca tttgcagaaa tcaaacgaaa ctttaaaact ttaaattctg acttaaaatt
     8101 aacaggcaac aacttcaaat ataccgaaaa atcaactgat agttacaaac aaaggattaa
     8161 agaacttgat ggaactatca caggttataa gaaaaacgtt gatgatttag ctaagcaata
     8221 tgacaaggta tctcaagaac agggcgaaaa cagtgcagga gctcaaaagt tacgacaaga
     8281 atataacaaa caagcaaatg aactgaatta tttagaaaga gaattacaaa aaacatcagc
     8341 cgaatttgaa gagttcaaaa aagctcaagt tgaagctcaa agaatggcag aaagtggctg
     8401 gggaaaaacc agtaaagttt ttgaaagtat gggacctaaa ttaacaaaaa tgggtgatgg
     8461 tttaaaatcc attggtaaag gcatgatgat tggtgtaact gcacctgttt taggtatcgc
     8521 agcagcatca ggaaaagctt ttgcagaagt tgataaaggt ttagatactg ttactcaagc
     8581 aacaggcgca acaggcagtg aattaaaaaa attgcagaac tcatttaaag atgtttatgg
     8641 caattttcca gcagatgctg aaactgttgg tggagtttta ggagaagtta atacaagatt
     8701 aggttttaca ggtaaagaac ttgaaaatgc cacagagtca ttcttgaaat tcagtcatat
     8761 aacaggttct gacggtgtgc aagccgtaca gttaattacc cgtgcaatgg gcgatgcagg
     8821 tatcgaagca agtgaatatc aaagtgtttt ggatatggta gcaaaagcgg cgcaagctag
     8881 tgggataagt gttgacacat tagctgatag tattactaaa tacggcgctc caatgagagc
     8941 tatgggcttt gagatgaaag aatcaattgc tttattctct caatgggaaa agtcaggcgt
     9001 taatactgaa atagcattca gtggtttgaa aaaagctata tcaaattggg gtaaagctgg
     9061 taaaaaccca agagaagaat ttaagaagac attagcagaa attgaaaaga cgccggatat
     9121 agctagcgca actagtttag cgattgaagc atttggtgca aaggcaggtc ctgatttagc
     9181 agacgctatt aaaggtggtc gctttagtta tcaagaattt ttaaaaacta ttgaagattc
     9241 ccaaggcaca gtaaaccaaa catttaaaga ttctgaaagt ggctccgaaa gatttaaagt
     9301 agcaatgaat aaattaaaat tagtaggtgc tgatgtatgg gcttctattg aaagtgcgtt
     9361 tgctccagtc atggaagaat taataaaaaa gctatcagtc gctgtcgatt ggttttcgaa
     9421 tttaagtgat ggttctaaaa gatcaattgt tattttcggt ggtattgctg ctgcaattgg
     9481 tcctgtagtt tttgggttag gtgcatttat aagcacaatt ggtaatgcag taactgtatt
     9541 agccccacta ttagctggta ttgcaaaggc tgatggatta attagttttt tatcgactaa
     9601 agtacctata ttaggaactg tcttcacagc tttaactggt ccaattggca ttgtattagg
     9661 tgttttagct ggcctagcag tcgcatttac aattgcttat aagaaatctg aaacatttag
     9721 aaattttgtt aatgatgcaa ttgaaagtgt taaacaaaca tttagtaatt ttattcaatt
     9781 tattcaacct ttcattgatt ccgttaaaaa cgtctttaaa caagcggttt cagcaatcgt
     9841 tgatttcgct aaagatattt ggagtcaaat taatggattc tttaatgaaa acggaatttc
     9901 tattgttcaa gcgcttcaaa atatatgcaa ttttatcaaa gttatatttg aatttatctt
     9961 aaattttgta attaaaccaa taatgtttgc aatctggcaa gtgatgcaat ttatttggcc
    10021 ggcggttaaa gccttgattg tcagtacttg ggagaatata aaaggtgtga tacaaggaac
    10081 tttaaatatc atactaggtt taattaagtt cttctcaagt ttatttactg gagattggcg
    10141 aggagtttgg gacgcgatta ttatgattct taaaggagtc gttcaattaa tatggaattt
    10201 aattcaatta tggtttgtag gcaaaatact tggcgttgtt aggtactttg gcggattgct
    10261 aaaaggatta ataacaggta tttgggacgt aataaaaagt atattcagta aatctttatc
    10321 agcaatttgg aatgcgacaa aaagtatttt tggattctta tttaatagtg tcaaatcaat
    10381 tttcacgaat atgaaaaatt ggttatctaa cacatggagt agtatccgta cgaatacgat
    10441 aggaaaagca cagtcattat ttagtggtgt gaaatcaaaa ttcactagtt tgtggaatgc
    10501 gacaaaagat atttttagta atctaagaaa ttggatggca aatatttgga attccattaa
    10561 agataatacg gttggaattg caagtcgttt atggagtaaa gtgcgtggaa tttttacaaa
    10621 tatgcgtgac ggcttacaaa gtattatcag caaaattaaa agtcatatcg gcggtatggt
    10681 agatgctatt aaaaaaggac ttaataaatt aattgaaggt ttaaactggg tcggtggtaa
    10741 gctgggaatg gataaaatac ctaagttaca cactggtaca gaacacacac atactactac
    10801 aagattagtt aagaacggta agattgcgcg ggatacgttc gctacggttg gggataaagg
    10861 acgtggaaat ggtccgaatg gtttcagaaa tgaaataatt gaattcccta atggtaaacg
    10921 tgtaatcaca cctaatacag atactaccgc ttatttacct aaaggctcaa aagtatacaa
    10981 cggtacacaa acttattcaa tgttaaacgg aacgcttcca agatttagct taggtactat
    11041 gtggaaagat attaaatccg gtgcatcatc ggcatttaac tggacaaaag atcaaatagg
    11101 taaaggtacc aaatggcttg gcgataaagt tggcgatgtt ttagatttta tggaaaatcc
    11161 tggcaaactt ttaaattata tacttgaagc ttttggaatt gatttcaatt ctttaactaa
    11221 aggtatggga attgcaggcg acataacaaa agctgcatgg tctaagatta agaaaagtgc
    11281 tactgattgg ataaaagaaa atttagaagc tatgggcggt ggcgatttag tcggcggaat
    11341 attagaccct gacaaaatta attatcatta tggacgtacc gcagcttata ctgctgcaac
    11401 tggaagacca tttcatgaag gtgtcgattt tccatttgta tatcaagaag ttagaacgcc
    11461 gatgggtggc agacttacaa gaatgccatt tatgtctggt ggttatggta attatgtaaa
    11521 aattactagt ggagttatcg atatgctatt tgcgcatttg aaaaacttta gcaaatcacc
    11581 acctagtggc acgatggtaa agcccggcga tgttgttggt ttaactggta ataccggatt
    11641 tagtacagga ccacacttac attttgaaat gagaagaaat ggacgtcact ttgaccctga
    11701 accatattta agaaatgcaa agaaaaaagg taggttatca attggtggcg gtggtgctac
    11761 ttctggaagt ggtgcaactt atgcaagccg agtaatccgt caagcgcaaa gtattttagg
    11821 aggacgttat aaaggtaagt ggattcatga ccagatgatg cgagttgcaa agcgtgaaag
    11881 taactatcaa tcaaatgcag tgaataattg ggatattaat gctcaaagag gagacccatc
    11941 aagaggatta ttccaaatca tcggctcaac ttttagagca aacgctaaac gtggatatac
    12001 taactttaat aatccggttc atcaaggtat ttcagcaatg cagtacattg ttagacgcta
    12061 cggttgggat ggatttaagc gtgctggtga ttacgcatat gctacaggtg gaaaagtttt
    12121 tgatggttgg tataacttag gtgaagacgg tcatccagaa tggattattc caacagatcc
    12181 agctcgtaga aatgatgcaa tgaagatgtt gcattatgca gcagcagaag taagagggag
    12241 aaaagcgagt aaaaataagc gtcctagtca attgtctagt gtaaatgggt ttgatgaccc
    12301 aagcttatta ttgaaaatga ttgaacaaca gcaacaacaa atagctttat tactgaaaat
    12361 agcgcaatcc aacgatgtga ttgcagataa agattatcag ccgattattg acgaatacgc
    12421 ttttgataaa aaggtgaacg cgtctataga aaagcgagaa aggcaagaat caacaaaagt
    12481 aaagtttaga aaaggaggaa ttgctattca atgatagaca ctattaaagt gaacaacaaa
    12541 acaattcctt ggttgtatgt cgaaagaggg tttgaaatac cctcttttaa ttatgtttta
    12601 aaaactgaaa atgtagatgg acgttcgggg tctatatata aagggcgtag gcttgaatct
    12661 tatagttttg atataccttt ggtggtacgt aatgactatt tatctcacaa cggctttaaa
    12721 acacatgatg acgtcttgaa tgaattagta aagtttttta actacgagga acaagttaaa
    12781 ttacaattca aatctaaaga ttggtactgg aacgcttatt ttgaaggacc aataaagctg
    12841 cacaaagaat ttgcaatacc tgttaagttc actatcaaag tagtactaac agacccttac
    12901 aaatattcag taacaggata taaaaatact gcgatttcag accaagtttc agttgtaaat
    12961 agtgggactg ctgacactcc tttaattgtt gaagcccgag caattaaacc atctagttac
    13021 tttatgatca ctaaaaatga tgaagattat tttatggttg gtgatgatga ggtaaccaaa
    13081 gaagttaagg attacatgcc tcctgtttat catagtgagt ttcgtgattt caaaggttgg
    13141 actaagatga ttactgaaga tattccaagt aatgatttag gtggtaaagt cggcggtgac
    13201 tttgtgatat ccaatcttgg cgaaggatat aaagcaacta atttttctga tgcaaaaggt
    13261 tgggttggtg ctggcacgaa acgagggctc cctaaagcga tgacagattt tcaaattacc
    13321 tataaatgta ttgttgaaca aaaaggtaaa ggtgccggaa gaacagcaca acatatttat
    13381 gatagtgatg gtaagttact tgcttctatt ggttatgaaa ataaatatca tgatagaaaa
    13441 ataggacata ttgttgttac gttgtataac caaaaaggag accccaaaaa gatatacgac
    13501 tatcagaata aaccgataat gtataacttg gacagaatcg ttgtttatat gcggctcaga
    13561 agagtaggta ataaattttc tattaaaact tggaaatttg atcacattaa agacccagat
    13621 agacgtaaac ctattgatat ggatgagaaa gagtggatag atggcggtaa gttttatcag
    13681 cgtccagctt ctatcatagc tatctatagt gcgaagtata atggttataa gtggatggag
    13741 atgaatggat taggttcatt caatacggag attctaccaa aaccgaaagg cgcaagggat
    13801 gtcattatac aaaaaggtga tttagtaaaa atagatatgc aagcaaaaag tgttgtcatc
    13861 aatgaggaac caatgttgag cgagaaatcg tttggaagta attatttcaa tgttgattct
    13921 gggtacagtg aattaatcat acaacctgaa aacgtctttg atacgacggt taaatggcaa
    13981 gatagatatt tatagaaagg agatgagagt gtgatacatg ttttagattt taacgacaag
    14041 attatagatt tcctttctac tgatgaccct tccttagtta gagcgattca taaacgtaat
    14101 gttaatgaca attcagaaat gcttgaactg ctcatatcat cagaaagagc tgaaaagttc
    14161 cgtgaacgac atcgtgttat tataagggat tcaaacaaac aatggcgtga atttattatt
    14221 aactgggttc aagatacgat ggacggctac acagagatag aatgtatagc gtcttatctt
    14281 gctgatataa caacagctaa accgtatgca ccaggcaaat ttgagaaaaa gacaacttca
    14341 gaagcattga aagatgtgtt gagcgataca ggttgggaag tttctgaaca aaccgaatac
    14401 gatggcttac gtactacgtc atggacttct tatcaaacta gatatgaagt tttaaagcaa
    14461 ttatgtacaa cctataaaat ggtattggat ttttatatag agcttagttc taataccgtc
    14521 aaaggtagat atgtggtact caaaaagaaa aacagcttat tcaaaggtaa agaaattgag
    14581 tatggtaaag atttggttgg gttaactagg aagattgata tgtcagaaat caaaacagca
    14641 ttaattgctg tgggacccga aaatgacaaa ggaaagcgtt tagagctagt tgtgacagat
    14701 gacgaagcgc aaagtcaatt caacctacct atgcgctata tttgggggat atatgaacca
    14761 caatcagatg atcaaaatat gaatgaaaca cgattaagtt ctttagccaa aacagagtta
    14821 aataaacgta agtcggcagt tatgtcatat gagattactt ctactgattt ggaagttacg
    14881 tatccgcacg agattatatc aattggcgat acagtcagag taaaacatag agattttaac
    14941 ccgccattgt atgtagaggc agaagttatt gctgaagaat ataacataat ttcagaaaat
    15001 agcacatata cattcggtca acctaaagag ttcaaagaat cagaattacg agaagagttt
    15061 aacaagcgat taaacctaat acaccaaaaa ttaaacgaca atattagcaa tatcaatact
    15121 atagtaaaag atgttgtaga tggtgaatta gaatactttg aacgcaaaat tcataaaagt
    15181 gatacaccgc cagaaaatcc agtcaatgat acgctttggt atgatacaag taaccctgat
    15241 gttgctgtct tgcgtagata ttggaatggt cgatggattg aagcaacacc aaatgatgtt
    15301 gaaaaattag gtggtataac aagagagaaa gcgctattca gtgaattaaa caatattttt
    15361 attaatttat ctatacaaca cgctagtctt ttgtcagaag ctacagaatt actgaatagc
    15421 gagtacttag tagataatga tttgaaagcg gacttacaag caagtttaga cgctgtgatt
    15481 gatgtttata atcaaattaa aaataattta gaatctatga cacccgaaac tgcaacgatt
    15541 ggtcggttgg tagatacaaa aactttattt cttgagtata gaaagaaatt acaagatgtt
    15601 tatacagatg tagaagatgt caaaatcgcc atttcagata gatttaaatt attacagtca
    15661 caatacactg atgaaaaata taaagaagcg ttggaaataa tagcaacaaa atttggttta
    15721 acggtgaatg aagatttgca gttagtcgga gaacctaatg ttgttaaatc agctattgaa
    15781 gcagctagag aatccacaaa agaacaatta cgtgactatg taaaaacatc ggactataaa
    15841 acagacaaag acggtattgt tgaacgttta gatactgctg aagctgagag aacgacttta
    15901 aaaggtgaaa tcaaagataa agttacgtta aacgaatatc gaaacggatt ggaagaacaa
    15961 aaacaatata ctgatgacca gttaagtgat ttgtccaata atcctgagat taaagcaagt
    16021 attgaacaag caaatcaaga agcgcaagaa gctttaaaat catacattga tgctcaagat
    16081 aatcttaaag agaaggaatc gcaagcgtat gctgatggta aaatttcgga agaagagcaa
    16141 cgcgctatac aagatgctca agctaaactt gaagaggcaa aacaaaacgc agaactaaag
    16201 gctagaaacg ctgaaaagaa agctaatgct tatacagaca acaaggtcaa agaaagcaca
    16261 gatgcacaga ggagaacact gactcgctat ggttctcaaa ttatacaaaa tggtaaggaa
    16321 atcaaattaa gaactactaa agaagagttt aatgcaacca atcgtacact ttcaaatata
    16381 ttaaacgaga ttgtccaaaa cgttacagat ggaacaacaa tcagatatga tgataacgga
    16441 gtggctcaag ctttaaatgt ggggccacgt ggtattagat taaatgctga taaaattgat
    16501 attaacggta atagagaaat aaaccttctt atccaaaata tgcgagataa agtagataaa
    16561 accgatattg tcaacagcct taatttatca agagagggtc ttgatatcaa tgttaataga
    16621 attggaatta aaggcggtaa caataacaga tatgttcaaa tacagaatga ttctattgaa
    16681 ctaggtggta ttgtgcaacg aacttggaaa ggcaaacgat caaccgatga tatattcaca
    16741 cgtcttaaag atggacatct aaggtttaga aataataccg caggcggttc actttatatg
    16801 tcacattttg gtatttcaac atatattgat ggagaaggcg aagacggagg ttcatccggt
    16861 actattcaat ggtgggataa aacttacagt gatagcggta tgaatggcat aacaatcaat
    16921 tcctatggtg gtgtcgttgc actaacgtca gataataatc gggttgttct ggagtcttac
    16981 gcttcatcga atatcaaaag caaacaggca ccggtgtatt tatatccaaa cacagacaaa
    17041 gtgcctggat taaaccgatt tgcattcacg ctgtctaatg cagataatgc ttattcgagt
    17101 gacggttata ttatgtttgg ttctgatgag aactatgatt acggtgcggg tatcaggttt
    17161 tctaaagaaa gaaataaagg tcttgttcaa attgttaatg gacgatatgc aacaggtgga
    17221 gatacaacaa tcgaagcagg gtatggcaaa tttaatatgc tgaaacgacg tgatggtaat
    17281 aggtatattc atatacagag tacagaccta ctgtctgtag gttcagatga tgcaggagat
    17341 aggatagctt ctaactcaat ttatagacgt acttattcgg ccgcagctaa tttgcatatt
    17401 acttctgctg gcacaattgg gcgttcgaca tcagcgcgta aatacaagtt atctatcgaa
    17461 aatcaatata acgatagaga tgaacaactg gaacattcaa aagctattct taacttacct
    17521 attagaacgt ggtttgataa agctgagtct gaaattttag ctagagagct gagagaagat
    17581 agaaaattat cggaagacac ctataaactt gatagatacg taggtttgat tgctgaagag
    17641 gtggagaatt taggattaaa agagtttgtc acgtatgatg acaaaggaga aattgaaggt
    17701 atagcgtatg atcgtctatg gattcatctt atccctgtta tcaaagaaca acaactaaga
    17761 atcaagaaat tggaggagtc aaagaatgca ggataacaaa caaggattac aagctaatcc
    17821 tgaatataca attcattatt tatcacagga aattatgagg ttaacacaag aaaacgcgat
    17881 gttaaaagcg tatatacaag aaaataaaga aaatcaacaa tgtgctgagg aagagtaatc
    17941 tttagcacta tttttataca aaaatttaag gaggtcattt aattatggca aaagaaatta
    18001 tcaacaatac agaaaggttt attttagtac aaatcgacaa agaaggtaca gaacgtgtag
    18061 tatatcaaga tttcacagga agttttacaa cttctgaaat ggttaaccat gctcaagatt
    18121 ttaaatctga agaaaacgct aagaaaattg cggagacgtt aaatttgtta tatcaattaa
    18181 ctaacaaaaa acaacgtgtg aaagtagtta aagaagtagt tgaaagatca gatttatctc
    18241 cagaggtaac agttaacact gaaacagtat gaaaagctat gagttagata ctcataatct
    18301 ttattctttt agaaagcggg tgtactgaat tggggtggtt caaaaaacac gaacatgaat
    18361 ggcgcatcag aaggttagaa gagaatgata aaacaatgct cagcacactc aatgaaatta
    18421 aattaggtca aaaaacccaa gagcaagtta acattaaatt agataaaacc ttagatgcta
    18481 ttcaaaaaga aagagaaata gatgaaaaga ataagaaaga aaatgataag aacatacgtg
    18541 atatgaaaat gtgggtgctt ggtttagttg ggacaatatt tggatcgcta attatagcat
    18601 tattgcgtat gcttatgggc atataagaga ggtgaataaa atgtttaaac taatctttgg
    18661 ttatagtttc tggacatgtt tttggttcgg taaatgtaaa taagttttag tcagtgcttc
    18721 ggtactgact ttttatttat tgttgtaatt atggtaatat gcagaagtga gcaagttgga
    18781 tagatggtgg ctatctgagt ataaggaggt ggtgcctatg gtggcattac tgaaatcttt
    18841 agaaaggaga cgcctaatga ttacaattag taccatgttg cagtttggtt tattccttat
    18901 tgcattgata ggtctagtaa tcaagcttat tgaattaagc aataaaaaat aaccatcgct
    18961 aactttggct ggtttcgatg gttaaatggt tattaattta atctttaatc taaaatagcc
    19021 accgtctttt taacgggctc attagggtaa catgtttgcg catgttgccc tttttctata
    19081 tataaattaa cacaccataa tataaatatc aaatagacgg cttattagtc gtctttttat
    19141 tttgggtaaa aggagataag aatatgatta attggaaaat tagaatgaaa caaaaatcat
    19201 tttgggtagc gatattgtca gctatctttt tatttgctca aaacatcgca aaagctattg
    19261 ggtatgatat ccaagtttat acagagcaat taacagacgg tttaaacgct atattaggat
    19321 ttttagtatt aactggtgtg attcaagacc cgactactaa aggtataggt gatagccacc
    19381 aagctttaga atatgaagaa ccaagaagaa aatactagga ggtaaaataa tgaaaacata
    19441 cagtgaagca agagcaaggt tacgttggta tcaaggtaga tatattgatt ttgacggttg
    19501 gtatggttac caatgtgcag atttagcagt tgattacatt tattggttgt tagaaattag
    19561 aatgtgggga aatgcaaaag atgcaatcaa taacgatttt aaaaacatgg caacagtata
    19621 tgaaaacaca ccatcgtttg ttccacaaat aggtgatgtg gctgtattta ccaaaggaat
    19681 atataaacaa tacggtcata ttggtttagt gtttaatggt ggtaatacaa accaattttt
    19741 aattttggaa cagaactatg acggtaacgc aaatacgcct gcaaagttac gttgggataa
    19801 ttattacggc tgtactcact ttattagacc taagtataaa agtgagggct taatgaataa
    19861 gatcacaaat aaagttaaac cacctgctca aaaagcagtc ggtaaatctg caagtaaaat
    19921 aacagttgga agtaaagcgc cttataacct taaatggtca aaaggtgctt attttaatgc
    19981 gaaaatcgac ggcttaggtg ctacttcagc cactagatac ggtgataatc gtactaacta
    20041 tagattcgat gttggacagg ctgtatacgc gcctggaaca ttaatatatg tgtttgaaat
    20101 tatagatggt tggtgtcgca tttattggaa caatcataat gagtggatat ggcatgagag
    20161 attgattgtg aaagaagtgt tttaattctt aggttaaaat gttaaatatt tgttaattat
    20221 tttttaatgt aattttagtt tcttttaata ttttattgat ttttaatatt ttctcaatat
    20281 aaaatgaagt tgttgatatt tatcatctta aataagggtg ttagctataa aaagagataa
    20341 ataaaaacaa atatattata tttggaggaa gcgccatgct caaaagaggt ttattatttt
    20401 taactgtttt attgttatta ttctcatttt cttcaattac taatgaggta agtgcatcaa
    20461 gttcattcga caaaggaaaa tataaaaaag gcgatgacgc gagttatttt gaaccaacag
    20521 gcccgtattt gatggtaaat gtgactggag ttgatggtaa aggaaatgaa ttgctatccc
    20581 ctcattatgt cgagtttcct attaaacctg ggactacact tacaaaagaa aaaattgaat
    20641 actatgtcga atgggcatta gatgcgacag catataaaga gtttagagta gttgaattag
    20701 atccaagcgc aaagatcgaa gtcacttatt atgataagaa taagaaaaaa gaagaaacga
    20761 agtctttccc tataacagaa aaaggttttg ttgtcccaga tttatcagag catattaaaa
    20821 accctggatt caacttaatt acaaaggtta ttatagaaaa gaaataaaac aaaatagttg
    20881 tttattatag aaagcaatgt cttgattgaa tatgtgtagt gaaaattatc tttcatcaaa
    20941 ttctcattca tgcacgaatg gttcttcccc acctaatcag atattaggtg acttatgggg
    21001 agaaatcagt taggatgaaa aagtggataa tcctttttta ggcaggtact tcggtacttg
    21061 cctatttttt tatgttataa tctttctaga cgtattcaag ggacgtcttt ttagattgta
    21121 tgttatagct agctttcggg ctagtttttt gttatgatgt gttacacatg catcaactat
    21181 ttacatctat ccttgttcac ccaagcatgt cactgggtgt tttttcttat gatagagagc
    21241 atagttttca tactactccc tcgtagtata tatgacttta gcattcccgt ataatagttt
    21301 acggggtgct ttttatgtta taattaactg tatatagtag gagtgaacta tatagcctgt
    21361 taagtggcct agtaacctaa cacttatcct gcaattgata tcctttttgc ccttcactcg
    21421 atacatatat ctcaacaaca tagaaatatt acagtcgcta caccgcatct taaatggtgt
    21481 ggttattttt attggaagtg tgtatcaggt atcagtaatg ttaaaacacc agctaaaaat
    21541 gaaaagaatt caccagtgcc agcaggttat acactcgata aaaacaatgt accgtataaa
    21601 aaagagactg gttattacac agttgccaat gttaaaggta ataacgtgag ggatggctat
    21661 tcaactaatt caagaattac aggtgtatta cccaataacg caactatcaa atatgacggc
    21721 gcatattgca ttaatggcta tagatggatt acttatattg ctaatagtgg acaacgtcgt
    21781 tatatagcga caggagaggt agacaaggca ggtaatagaa taagcagttt tggtaagttt
    21841 agtgcagttt gataattaga tatataaag
//
LOCUS       DAKTIO010000025        21733 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.025, whole genome shotgun sequence.
ACCESSION   DAKTIO010000025 DAKTIO010000000
VERSION     DAKTIO010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21733
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.025"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..1242)
                     /locus_tag="O0293_000731"
     CDS             complement(<1..1242)
                     /locus_tag="O0293_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCY0279628.1"
                     /translation="MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSM
                     AAIVLVTFVWITVGFTISFGNGNLWFGNWEYTFLNHVGFATQEDISPHIPFALFMLFQ
                     MMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFA
                     GGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFT
                     FDNIAMLAFTNTVISASAGAIGWLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVT
                     YLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGIIGAVLTAVFQSKKAN
                     PDIENGFIYTGDIHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGL
                     DKIVHGEHAYFEGELNRFNKHI"
     gene            complement(1450..1674)
                     /locus_tag="O0293_000732"
     CDS             complement(1450..1674)
                     /locus_tag="O0293_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCY0279629.1"
                     /translation="MIHELGTVGMVCPFPLIEAQKKMATLQSGDELKIDFDCTQATEA
                     IPNWAAENGYPVTNYEQIDNASWTITIQKV"
     gene            complement(1734..2813)
                     /locus_tag="O0293_000733"
     CDS             complement(1734..2813)
                     /locus_tag="O0293_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HCY0279630.1"
                     /translation="MWMIFSGLIVGGLLGFVMQRTRFCLTGGFRDMYVQKNNKMFYAL
                     LIAITIQSIGLLILTATDILQIPAHSFPILGTIIGSFIFGIGIVLAGGCATGTWYRAG
                     EGLIGSWIALVLYAVTAAITKTGILKPVMDKINQPTNVNSDMSQTTGIPFWGLVVILT
                     IITIFLVVRTLNNKKVRVAVPKLKQRYTGIRYYLFEKRYHPFIAAIVIGLIALLAWPM
                     SASTGRNDGLGITTPSANLVHFLITGETKFIDWGVFLVLGIFIGSYIAARGSREFKWR
                     LPDKITIRNSAIGGICMGFGASVAGGCSIGNGLVETATMTWQGWIALASMIVGVWTMS
                     HFIFVRPMKKVHQQSAKVKQQTQIV"
     gene            complement(3117..3752)
                     /locus_tag="O0293_000734"
     CDS             complement(3117..3752)
                     /locus_tag="O0293_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCY0279631.1"
                     /translation="MSDQVKIPRATLKRLPLYYRFVSSLKSKGIDRVNSKAISDALQI
                     DSATIRRDFSYFGELGKKGYGYNIDSLLDFFKSELSESDMIKIAIVGVGNLGKALLTY
                     NFSIHDDMTITEAFDVKEDVIGQKIGNVIVKDNDELITTLKKEEIDVVILTTPERVAQ
                     KVADELVQAGVKGILNFTPGRINTPSDVQVHQIDLGIELQSLLFFMKNYSE"
     gene            4005..5933
                     /gene="abc-f"
                     /locus_tag="O0293_000735"
     CDS             4005..5933
                     /gene="abc-f"
                     /locus_tag="O0293_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HCY0279632.1"
                     /translation="MILLQLNHISKSFDGEDIFTDVDFEVKTGERIGIVGRNGAGKST
                     LMKIIAGVENYDSGNVSKIKNLKLGYLTQQMTLNSNATVFEEMSKPFEHIKRMESLIK
                     EETDCLSKHANDYDSDTYKTHMSRYESLSNQFEQLEGYQYESKIKTVLYGLNFSEEDF
                     NKPINDFSGGQKTRLSLAQMLLNEPDLLLLDEPTNHLDLETTKWLEDYLRYFKGAIVI
                     ISHDRYFLDKIVTQIYDVALGDVKRYVGNYEEFIQQRDLYYQKRMQEYESQQAEIKRL
                     ETFVEKNITRASTSGMAKSRRKILEKMERIDKPMLDAKSANIQFGFDRNTGNDVMHVK
                     NLEIGYQTAITKPMSIEVSKGDHIAIIGPNGIGKSTLIKTIANQQKALNGDITFGANL
                     QIGYYDQKQAEFKSSKTILDYVWDQYPLMNEKDIRAVLGRFLFVQDDVKKIINDLSGG
                     EKARLLLALLMLQRDNVLILDEPTNHLDIDSKEMLEQALQHFEGTILFVSHDRYFINQ
                     LANKVFDLTIDGGKMYLGDYQYYIEKVEEAEALKAHQEEQSVNVQAHKKSMEQSSYHN
                     QKEQRREQRKLERQISECENEIETLETTILQIDEQLTQPEVYNNPQKANELAIQKQDS
                     EQKLEHAMSKWEELQQKL"
     gene            complement(6077..6205)
                     /locus_tag="O0293_000736"
     CDS             complement(6077..6205)
                     /locus_tag="O0293_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279633.1"
                     /translation="MIKTMSIENMRTYQQRVNNLWIKGVDNVIYCGYQVGKQKIND"
     gene            6294..7904
                     /locus_tag="O0293_000737"
     CDS             6294..7904
                     /locus_tag="O0293_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HCY0279634.1"
                     /translation="MSTNQTFLIFVIAIILLTSVIGIVGRYMSRQRLLKSMETLWQTI
                     SPLEAFIRPNSHFDYEYKLYKEKFESHSLVDDKTWSDLNMNAIFHKMNYNLTAIGEMK
                     LYACLRGMLSITNKSLLSLFNDNAEFRKNVTYHLALIGKTVYPTFPDQITPVKRQNIL
                     FLCPFLPVISFAVIFINSQVGILLFLMSCLFNIILSATLKRTYEDDLKSIFYASNVLK
                     QGYTISKIKHAPQPEVNFKQFRTARHLTSVLAEVNDEDIGAMVIKLVKLIFMLDYVLF
                     HSIQKSYTTHMNELKNCFDYIAELDNHYALAMYRRTLECYTEPQIDDSNDGIVFSELT
                     HPLIADAVANDFSLSQNILLTGSNASGKSTFMKSIAINIILASAIQTVTASKFVYQPG
                     IVFTSMANADDVLSGDSYFMAELKSIKRIVEIPDNQKIYCFIDEIFKGTNTTERIAAS
                     ESVLSFLHEKSNFRVIAATHDIELAELLKQRYENYHFNEVIENNNIHFDYKIKPGKAN
                     TRNAIELLKITSFPAKIYERAKDNVPKI"
     gene            complement(8006..9031)
                     /gene="tsaD"
                     /locus_tag="O0293_000738"
     CDS             complement(8006..9031)
                     /gene="tsaD"
                     /locus_tag="O0293_000738"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCY0279635.1"
                     /translation="MTKDILILAVETSCDETSVSVIKNGRDILSNTVLSQIESHKRFG
                     GVVPEVASRHHVEGITATINEALGDADVSIEDIDAIAVTEGPGLIGALLIGVNAAKAL
                     AFAYDKPLIPVHHIAGHIYANHIEEPLTFPLIALIVSGGHTELVYMKDHLSFEVIGET
                     RDDAVGEAYDKVARTIGLNYPGGPQVDRLAAEGEDTYSFPRVWLDKDSYDFSFSGLKS
                     AVINQLHNQRQKNIPIIEANVATSFQNSVVEVLTFKAIQACKEYGVQRLIVAGGVASN
                     KGLRQSLADQCKVNDIQLTIPSPKLCTDNAAMIGVAGHYLYQQGRFADLALNGHSNID
                     LEEYSAE"
     gene            complement(9024..9488)
                     /gene="rimI"
                     /locus_tag="O0293_000739"
     CDS             complement(9024..9488)
                     /gene="rimI"
                     /locus_tag="O0293_000739"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCY0279636.1"
                     /translation="MDQQSKEQLNIREMTKEDVPQVFDLERRSFNDSSWTIDAFYHEI
                     EQNNFAKYFVLEFEQQIIGYLGLWIVIDQAQITTVAIDDNYRGYGLGQMLLKYGINYA
                     SHTCDVMSLEVRVNNKVAQHVYENLGFQYGGKRKNYYGEGEDAMVMWVNLND"
     gene            complement(9461..10123)
                     /gene="tsaB"
                     /locus_tag="O0293_000740"
     CDS             complement(9461..10123)
                     /gene="tsaB"
                     /locus_tag="O0293_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCY0279637.1"
                     /translation="MNSLLIDTSNQPLSVALMQNDEVLAEITTDSKQNHSVQLMPAIS
                     QLFEQSKIAKQQLDAIIVAEGPGSYTGLRIGVTVAKTLAYALDVKLYGVSSLKALAAT
                     IDHTDKLLVPVFDARRQAVYTGIFQWQNGQLVTILEDQYMSIEALRAFLEDLNQPFIY
                     IGKDTVKLQDELQGEVIAQLPNASVMYQLLDKPSDVHTFTPKYHKLAEAERNWINSQK
                     NN"
     gene            complement(10104..10565)
                     /gene="tsaE"
                     /locus_tag="O0293_000741"
     CDS             complement(10104..10565)
                     /gene="tsaE"
                     /locus_tag="O0293_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCY0279638.1"
                     /translation="MIKINNLDEMNQFAIFLVEQLKSGDLILLNGDLGAGKTTLTQFI
                     GKALGVRRTINSPTFNIIKSYRGKNLKLHHMDCYRLEDSDEDLGFDEFFEDQAITVIE
                     WSQFIKDLLPATHLSINISTISENTRQIELFAQGEHYEQIKEAIIHEFAAH"
     gene            10603..10758
                     /locus_tag="O0293_000742"
     CDS             10603..10758
                     /locus_tag="O0293_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279639.1"
                     /translation="MIIHCSIVSNFIFNLYIVTNFNLNFLSLKQIIYSKLIVRKFKIF
                     VDILKQI"
     gene            11076..12764
                     /gene="ilvD"
                     /locus_tag="O0293_000743"
     CDS             11076..12764
                     /gene="ilvD"
                     /locus_tag="O0293_000743"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCY0279640.1"
                     /translation="MRSDMIKKGDHQAPARSLLHATGALKSPTDMNKPFVAICNSYID
                     IVPGHVHLRELADIAKEAIREAGAIPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADA
                     AETVINAHWFDGVFYIPNCDKITPGMILAAMRTNVPAIFCSGGPMKAGLSAHGKALTL
                     SSMFEAVGAFKEGSISKEEFLDMEQNACPTCGSCAGMFTANSMNCLMEVLGLTLPYNG
                     TALAVSDQRREMIRQAAFKLVENIKNDLKPRDIVTREAIDDAFALDMAMGGSTNTVLH
                     TLAIANEAGIDYDLERINAIAKRTPYLSKIAPSSSYSMHDVHEAGGVPAIINELMKKD
                     GTLHPDRITVTGKTLRENNEGKEIKNFDVIHPLDAPYDAQGGLSILFGNIAPKGAVIK
                     VGGVDPSIKTFTGKAICFNSHDEAVEAIDNRTVRAGHVVVIRYEGPKGGPGMPEMLAP
                     TSSIVGRGLGKDVALITDGRFSGATRGIAVGHISPEAASGGPIALIEDGDEITIDLTN
                     RTLNVNQPEDVLARRRESLTPFKAKVKTGYLARYTALVTSANTGGVMQVPENLI"
     gene            12792..14561
                     /gene="ilvB"
                     /locus_tag="O0293_000744"
     CDS             12792..14561
                     /gene="ilvB"
                     /locus_tag="O0293_000744"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HCY0279641.1"
                     /translation="MSKTQHEVNQNIDPLKMAESLEPEQLNEKTLNDMRSGSEVLVEA
                     LLKENVDYLFGYPGGAVLPLYDTFYDGKIKHILARHEQGAVHAAEGYARVSGKTGVVV
                     VTSGPGATNVMTGITDAHCDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNY
                     QVKRVEDIPKIVHEAFHVANSGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTE
                     PENKDIDTFISLLKEAKKPVVLAGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVP
                     YEDTLFLGMGGMHGSYASNMALTECDLLINLGSRFDDRLASKPDAFAPNAKIVHVDID
                     PSEINKVIHVDLGIIADCKRFLECLNDKNVETIEHSDWVKHCQNNKQKHPFKLGEEDQ
                     VFCKPQQTIEYIGKITNGEAIVTTDVGQHQMWAAQFYPFKNHGQWVTSGGLGTMGFGI
                     PSSIGAKLANPDKTVVCFVGDGGFQMTNQEMALLPEYGLDVKIVLINNGTLGMVKQWQ
                     DKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFLIDKPEQLEEQLDAAFAYQGPALIEVR
                     ISPTEAVTPMVPSGKSNHEMEGL"
     gene            14561..14815
                     /locus_tag="O0293_000745"
     CDS             14561..14815
                     /locus_tag="O0293_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HCY0279642.1"
                     /translation="MTRILKLQVADQVSTLNRITSAFVRLQYNIDTLHVTHSEQPGIS
                     NMEIQVDIQDDTSLHILIKKLKQQINVLTVECYDLVDNEA"
     gene            14952..15956
                     /gene="ilvC"
                     /locus_tag="O0293_000746"
     CDS             14952..15956
                     /gene="ilvC"
                     /locus_tag="O0293_000746"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCY0279643.1"
                     /translation="MTTVYYDQDVKTDALQGKKIAVVGYGSQGHAHAQNLKDNGYDVV
                     IGIRPGRSFDKAKEDGFDVFPVAEAVKQADVIMVLLPDEIQGDVYKNEIEPNLEKHNA
                     LAFAHGFNIHFGVIQPPADVDVFLVAPKGPGHLVRRTFVEGSAVPSLFGIQQGASGQA
                     RNIALSYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGVSKLIQSGFETLVEAGY
                     QPELAYFEVLHEMKLIVDLMYEGGMENVRYSISNTAEFGDYVSGPRVITPDVKENMKA
                     VLTDIQNGNFSNRFIEDNKNGFKEFYKLREEQHGHQIEKVGRELREMMPFIKSKSIEK
                     "
     gene            15986..17515
                     /locus_tag="O0293_000747"
     CDS             15986..17515
                     /locus_tag="O0293_000747"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCY0279644.1"
                     /translation="MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIE
                     AGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSDIDAVYEATKDAAKPVVHVFIAT
                     SPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVD
                     AGATVINIPDTVGYSYHDEYAHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAI
                     EGGARRIEGTVNGIGERAGNAALEEVALALYVRNDHYGAQTALNLEETKKTSDLISRY
                     AGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVGVSTTELPLGKLSGK
                     HAFSEKLKALGYDIDKEAQIDLFKQFKAIADKKKSVSDRDIHAIIQGSEHEHQALYKL
                     ETLQLQYVSSGLQSAVVVVKDKEGHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYR
                     INSVTEGTDAQAEVHVNLLIEGKTVNGFGIDHDILQASCKAYVEAHAKFAAENVEKVG
                     N"
     gene            17518..18564
                     /gene="leuB"
                     /locus_tag="O0293_000748"
     CDS             17518..18564
                     /gene="leuB"
                     /locus_tag="O0293_000748"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCY0279645.1"
                     /translation="MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFG
                     GASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWTDPNNRPEQGLLKLRKSLNLFVN
                     IRPTTVVKGASSLSPLKEERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTRE
                     EIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDA
                     CSMHLITNPKQFDVIVCENLFGDILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGS
                     APDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYSMIEHGQTTADLGGKLN
                     TTDIFEILSQKLNH"
     gene            18578..19948
                     /gene="leuC"
                     /locus_tag="O0293_000749"
     CDS             18578..19948
                     /gene="leuC"
                     /locus_tag="O0293_000749"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCY0279646.1"
                     /translation="MGQTLFDKVWNRHVLYGKLGEPQLLYIDLHLIHEVTSPQAFEGL
                     RLQNRKLRRPDLTFATLDHNVPTIDIFNIKDEIANKQITTLQKNAIDFGVHIFDMGSD
                     EQGIVHMVGPETGLTQPGKTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQTLWQT
                     KPKNLKIDINGTLPTGVYAKDIILHLIKTYGVDFGTGYALEFTGETIKNLSMDGRMTI
                     CNMAIEGGAKYGIIQPDDITFEYVKGRPFADNFAKSVDKWRELYSDDDAIFDRVIELD
                     VSTLEPQVTWGTNPEMGVNFSEPFPEINDINDQRAYDYMGLEPGQKAEDIDLGYVFLG
                     SCTNARLSDLIEASHIVKGNKVHPNITAIVVPGSRTVKKEAEKLGLDTIFKNAGFEWR
                     EPGCSMCLGMNPDQVPEGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIHGKFVD
                     VRKVVV"
     gene            19949..20521
                     /gene="leuD"
                     /locus_tag="O0293_000750"
     CDS             19949..20521
                     /gene="leuD"
                     /locus_tag="O0293_000750"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCY0279647.1"
                     /translation="MAAIKPITTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPF
                     AFDEWRYLPDGSDNPDFNPNKPQYKGASILITGDNFGCGSSREHAAWALKDYGFHIII
                     AGSFSDIFYMNCTKNAMLPIVLEKSAREHLAQYVEIEVDLPNQTVSSPDKRFHFEIDE
                     TWKNKLVNGLDDIAITLQYESLIEKYEKSL"
     gene            20536..>21733
                     /gene="ilvA"
                     /locus_tag="O0293_000751"
     CDS             20536..>21733
                     /gene="ilvA"
                     /locus_tag="O0293_000751"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HCY0279648.1"
                     /translation="MTVKTTVSTKDIDEAFLRLKDIVKETPLQLDHYLSQKYDCKVYL
                     KREDLQWVRSFKLRGAYNAISVLSDEAKSKGITCASAGNHAQGVAYTAKKLNLNAVIF
                     MPVTTPLQKVNQVKFFGNSNVEVVLTGDTFDHCLAEALTYTSEHQMNFIDPFNNVHTI
                     SGQGTLAKEMLEQAKSDNVNFDYLFAAIGGGGLISGISTYFKTYSPTTKIIGVEPSGA
                     SSMYESVVVNNQVVTLPNIDKFVDGASVARVGDITFEIAKENVDDYVQVDEGAVCSTI
                     LDMYSKQAIVAEPAGALSVSALENYKDHIKGKTVVCVISGGNNDINRMKEIEERSLLY
                     EEMKHYFILNFPQRPGALREFVNDVLGPQDDITKFEYLKKSSQNTGTVIIGIQLKDHD
                     DLIQLKQ"
BASE COUNT         7466 a         3754 c         3655 g         6858 t
ORIGIN      
        1 aatatgttta ttgaatctat ttagctcacc ttcaaagtaa gcatgttcac cgtgaacaat
       61 cttgtctaat cctatattcg tttcttgttc cgtaacagat aatggtgtaa ttaatttaat
      121 tactttcgca ataataaacg tcatgactat actaaaaatt acaactgctg ttacacataa
      181 tatttgtaca agtataatat gtatgtcacc agtataaata aagccattct caatgtcagg
      241 attggctttt ttactttgga aaactgctgt taaaacagca ccaataatac caccaacacc
      301 atgaatacca aatgcatcta atgcatcatg atattttagt tttaccttga tgtaattaat
      361 gacaatataa caacagatac ctcctattaa agccattatt gttgcactaa gatatgttac
      421 atatcctgct gcaggagtaa tgacaactaa tcctgctaat gcaccgagta aaagtccaag
      481 taaacttgtc gtctttttaa aaatatattc taaaattaac caacctatag cacctgcact
      541 ggctgaaatg acagtatttg taaatgcaag catcgcaata ttatcaaatg taaaagcact
      601 acctacatta aatccatacc aaccaatcca cacgaatata ccgccaatca acgtaatgat
      661 aagattatgt ggtgttgatt cagaatgttt gtttcctttt ccaatcataa tagctaatac
      721 taaaccagaa acacctgatg taatatgaac aaccgtacct ccagcgaaat ctaatacacc
      781 gagtttgtta atccaaccgc cgccccaaac ccaatgtgct actggactgt atacaagagc
      841 agtccatatt actacgaata ataaataagg aataaacttc attttctcag cgattgaacc
      901 agataaaata gaaattgcaa tcgtacaaaa catcatttga aataacataa acaaagcgaa
      961 aggaatatgt gggctaatat cttcttgagt cgcaaaacct acatgattaa gaaaagtata
     1021 ttcccaattt ccgaaccata aattcccatt cccaaaacta attgtaaaac caactgttat
     1081 ccatacaaat gtaacaagca caattgctgc catactttgc atgacagtat taagcgcatt
     1141 tttagattga actaacccac cataaaataa acttaatcct ggtgtcatta accaaactaa
     1201 taatgtacac aaaaacataa atatcgtatc gttaagattc atacttacca ctcccttttt
     1261 ttcataataa tctgacaatt tataaaaagc aaaaatgcgt taagttttat tacatctttt
     1321 ggaataaaat gtgccacact atataaaaat gatttcatga actaaaacaa aaaagactac
     1381 ttactcactt ttaaattagc ggaagtagcc atagaatcta atatctattt tattgttaaa
     1441 atgataacgt taaacttttt gaattgtaat tgtccatgaa gcattatcaa tttgttcata
     1501 gtttgttaca ggataaccat tttctgcagc ccaatttgga atggcttccg tcgcttgcgt
     1561 gcaatcaaaa tcaattttta attcatctcc agattgcaat gttgccattt tcttttgcgc
     1621 ttcaattaac ggaaatggac ataccattcc tactgtacct aattcgtgta tcataattat
     1681 ttctcctttt ccaatcactt ttatttgatc aacatcattt gcataataat cttctatact
     1741 atttgcgttt gctgtttaac ctttgcagat tgttggtgta cttttttcat tggacgaaca
     1801 aagataaaat gactcattgt ccatacacca actatcatcg atgctagcgc aatccatcct
     1861 tgccaagtca tcgttgccgt ttcaaccaaa ccgttaccga tagaacaacc accagcaact
     1921 gacgcaccaa atcccataca tatgccacca atggcactgt ttcgtattgt aatcttgtct
     1981 ggcaatcgcc atttaaattc tcttgatcct ctagctgcaa tatatgaacc aatgaaaatt
     2041 cctagaacta aaaagacacc ccaatcaata aatttagttt cacctgtaat caaaaagtgt
     2101 actaaatttg ctgaaggcgt tgttatacct aaaccgtcat ttcttccagt tgatgcactc
     2161 attggccaag ctaagagtgc gataagtcca attacaattg ctgcaataaa tggatggtat
     2221 cgtttttcga aaaggtaata tctaatacct gtataacgtt gctttagttt tggaacagca
     2281 actcgaactt tcttattatt gagtgttctt acaactagaa aaatggtgat tatagttaat
     2341 ataacgacta atccccaaaa cggaatgcca gttgtttgag acatatcact atttacattc
     2401 gttggttgat taattttatc cattactggc tttaaaatcc ctgttttagt gattgctgca
     2461 gtaacagcat ataatactaa tgcaatccaa ctaccaatta gcccttcacc agcgcgatac
     2521 caagtacctg ttgcacatcc tccagccaat actattccaa ttccaaaaat aaaagaacct
     2581 ataattgttc ccaatattgg aaaactatgt gcaggaattt gtaaaatatc tgttgccgtc
     2641 aaaatcaata atcctatact ttgaatagta atagcgatta ataatgcata gaacatctta
     2701 ttattctttt gcacatacat atctcgaaag ccacctgtta aacaaaatct tgttctttgc
     2761 atgacaaaac ctaagagtcc cccgacaatt aagccactaa aaatcatcca cataatctta
     2821 acctctcatt tccgataagt ttagtatgta ttataggtga tgattttctt gttttcaacc
     2881 ttatacacaa tatttttgat atttatatta tattcatata aggaaggcga ttaattacga
     2941 ttcatcatta tatctctacg tgttaattta ttaacctata ctatatttat caacacaaca
     3001 gtttttcctt tcattttcaa acataacaga aaaagcctga gacatactta gtcccagact
     3061 gttctataat taattaaaga tataacaagg ttaatcattc aaagctaaca tcacatttat
     3121 tcactgtaat ttttcataaa gaataataat gactgtaatt ctatacctaa gtcaatttga
     3181 tgtacttgca catctgaagg cgtattaatt ctaccaggag tgaagtttaa aatacctttc
     3241 acaccagctt ggacgagttc atctgcaact ttctgtgcaa ctctttctgg tgtagttaga
     3301 atcacaacat ctatttcttc cttcttcaat gttgttatta attcatcgtt atctttaaca
     3361 ataacgttcc ctattttctg gccaataaca tcttctttta cgtcaaacgc ttctgtaatc
     3421 gtcatatcgt catgtattga aaagttatat gtgagcaaag ctttccctag gttcccaact
     3481 ccgacaattg cgattttgat catgtcactc tcgcttagtt cagatttaaa gaaatccaat
     3541 aaactatcta tattatatcc gtaccctttt ttacctaatt cgccaaaata tgaaaagtca
     3601 cgacgaattg ttgccgagtc aatttgtaac gcatcgctaa tcgcttttga atttacacga
     3661 tctatacctt tagattttaa tgaactgaca aatctataat ataacggcaa acgttttaaa
     3721 gttgctcgag gaattttaac ttggtcactc attcgctatt tcctccttcg tgtttgaatg
     3781 aattatatct atgtattcaa acgagttaga gcgtaattgt tagattatta aaaaacaaat
     3841 aattgattac aatgaatacc taaatattat acattattga attttaaaaa taaatatctt
     3901 agtttcaaga cggcgtttta tactgtaaaa tggttattaa tgtaataatt acgacttttt
     3961 tcataagtta ataaaatttg agaaagacga aaggtgaaga tcgaatgata cttttacaac
     4021 tcaatcatat atcaaaatcg ttcgatggtg aagatatatt tactgatgtt gattttgaag
     4081 taaaaactgg tgaacgaatt ggtatcgtag gaagaaatgg cgccggtaaa tcaacattga
     4141 tgaaaattat agctggcgta gaaaactatg attctggaaa tgtgtcaaaa attaaaaact
     4201 taaaactagg ttatttaact caacaaatga ctttaaattc taatgcaaca gtttttgaag
     4261 aaatgtcaaa accatttgaa catattaaac gaatggaaag cttaatcaaa gaagaaacag
     4321 attgtttatc aaaacatgca aatgattacg atagtgatac atataaaaca catatgtccc
     4381 gttatgaatc tttatcgaat caatttgaac aattagaagg atatcaatat gaaagtaaaa
     4441 ttaaaacagt actttacggg ttaaatttta gtgaagaaga tttcaataaa cctatcaatg
     4501 attttagcgg tggccaaaaa acacgtttat ctttagctca aatgctatta aacgaacctg
     4561 atttattact tttagatgaa cctactaacc acttagattt agaaacgaca aagtggcttg
     4621 aagattatct acgttatttt aaaggtgcaa tcgtcatcat cagccatgat cgttactttt
     4681 tagataaaat agttactcaa atttatgatg tggctttagg tgatgtcaaa cgctatgttg
     4741 gtaattacga ggaatttata cagcaacggg atttatatta tcaaaaacga atgcaagaat
     4801 atgaaagtca acaagcagaa ataaaacgat tagaaacttt tgttgagaaa aatattaccc
     4861 gtgcttcaac aagtggaatg gcaaaaagta gacgtaagat tttagaaaaa atggaacgca
     4921 ttgataaacc aatgttagat gccaaaagtg caaatattca atttggcttt gaccggaata
     4981 caggtaatga cgtcatgcat gtaaaaaatt tagaaatcgg ttatcaaact gcaattacca
     5041 aacctatgag tatagaggtc tctaaaggcg atcatatagc aatcattggg ccaaatggta
     5101 ttggaaaatc gaccttaatt aaaactattg ctaatcaaca aaaagcgctt aatggcgata
     5161 ttactttcgg cgcaaattta caaattggtt attatgatca aaagcaagca gaatttaaat
     5221 ctagtaaaac gattttagat tatgtgtggg atcaatatcc gttaatgaat gaaaaagata
     5281 ttcgagcagt tcttggacgt ttcttatttg tacaagacga tgttaaaaag ataattaatg
     5341 atttatctgg tggtgaaaaa gcacgtttac tactagcact acttatgttg caacgtgaca
     5401 atgtacttat tttagatgaa cctaccaacc acctcgatat agattcaaaa gaaatgttag
     5461 agcaagcact ccaacatttt gaaggaacaa tactatttgt ttcccatgat cgttacttta
     5521 ttaaccaatt agcaaacaag gtatttgatt taacaattga tggcggaaag atgtatttag
     5581 gagattatca atattacatt gaaaaagttg aggaagcgga agcgttgaaa gcgcaccaag
     5641 aagaacagtc agttaacgta caagcacata aaaagtctat ggaacaatcg tcgtatcata
     5701 accaaaaaga acaaagacgt gaacaacgca aacttgaacg acaaatatcc gagtgtgaaa
     5761 atgaaataga aactttagag actactatct tacaaataga cgagcaatta acccaaccag
     5821 aagtgtataa caatccacaa aaagcaaacg aattagctat ccaaaaacaa gatagcgaac
     5881 aaaaattaga acacgctatg tctaaatggg aagaattaca acaaaaacta taaaaatata
     5941 cataactcta actgtaaaat cgctatagtt agagttattt ttttacaaat tttaattttt
     6001 ttattcacaa taaaaacgct gatattatag ctatccacag acttatccta gttacccaca
     6061 agtaaattaa cactaatcaa tcatttattt tctgttttcc cacctgatat ccacaataaa
     6121 tgacgttatc aacacctttt atccacaagt tattcactct ttgttgataa gtacgcatgt
     6181 tctctattga cattgttttt atcaaaattc attgttaaga cattttcttt atgaaataat
     6241 aataatattg aagtatattt ttattattat taaaaataaa taaggggata cttatgagca
     6301 caaatcaaac atttttaata tttgttatag caattattct acttacatct gtaataggaa
     6361 ttgttggacg atacatgagt cgtcaacgtc tattaaaatc tatggaaaca ttatggcaaa
     6421 cgatttctcc attagaagct tttatcagac cgaactcaca tttcgactat gagtataagc
     6481 tctacaagga aaaatttgaa tcacattcat tagttgatga taaaacttgg tccgacttaa
     6541 atatgaatgc aatctttcat aagatgaatt ataatttaac agctattggt gaaatgaagc
     6601 tatatgcctg tttacgtgga atgctttcaa ttacgaacaa atcattactt agtttattta
     6661 atgataatgc tgaatttaga aaaaacgtaa catatcattt agctttgatt ggtaaaactg
     6721 tttatccaac atttccagac caaatcacac cggtaaaacg tcaaaatata ttgtttctat
     6781 gcccgttttt accagttatc tcatttgcag ttatatttat taattcacaa gttggtattt
     6841 tattatttct tatgagttgc ctatttaata tcattttatc agcaacttta aagcgtacat
     6901 atgaggatga cttaaaatct atcttttatg catccaatgt tttaaaacaa ggctatacta
     6961 tctcaaaaat taaacatgct ccacaacctg aagtcaattt taagcaattt agaacagcac
     7021 gtcatttaac gagtgtttta gctgaagtta atgatgaaga tatcggagct atggtaatta
     7081 aactagttaa acttattttc atgcttgatt atgttttatt tcattcaatt caaaaaagct
     7141 atacaacaca tatgaatgag cttaaaaatt gtttcgatta catagctgaa ttagataatc
     7201 attatgcatt agcaatgtat cgtagaacgc tagaatgtta tactgaacca caaattgatg
     7261 attcaaatga tggcatagta ttctctgagt taacacatcc actcatagca gatgcagtag
     7321 ccaatgattt ctcactatca caaaatatac tgttaactgg gtctaatgct tcgggtaaat
     7381 caacatttat gaaatccata gcaataaaca ttattcttgc atcggctata caaacagtta
     7441 ccgcaagcaa atttgtatat caacctggaa ttgtgttcac gtcaatggca aatgccgatg
     7501 atgtgttatc aggcgatagt tattttatgg ctgaattaaa atctattaag cgtattgttg
     7561 aaattccaga taatcaaaaa atatactgct ttatagatga aatttttaaa ggtaccaaca
     7621 caactgaacg aattgccgct tcagaatcag tactatcatt tttacatgaa aaatctaact
     7681 ttagagttat tgcagcaaca catgatattg agttagctga actcttaaaa caacgttatg
     7741 aaaattacca tttcaatgag gtaatagaaa ataataacat acattttgat tacaaaatta
     7801 agcctggcaa agcaaataca cgtaatgcca tcgaattatt aaaaatcact tcatttccag
     7861 caaaaatata tgaacgagca aaagataatg tcccgaaaat ttagcattta actttaaaca
     7921 taaaaacgtc agctatcaca tgacagaaga ctatgaacag tttcaataat gttcatagta
     7981 atcatgttaa taactgacgt ttattttatt ctgcagaata ctcttctaaa tctatattgc
     8041 tgtgcccatt taatgctaaa tcagcaaatc gaccttgctg atacaaatag tggccggcaa
     8101 cgcctatcat tgcagcatta tctgtgcata atttaggact tgggatagtt aattgaatgt
     8161 cattgacttt gcattgatcc gctaaagatt gacgtaatcc tttattactc gccacgccac
     8221 cagcaacaat taatcgctga acaccatatt ctttacaagc ttgaatagct ttaaacgtga
     8281 gcacctctac aacactgttt tgaaagctcg ttgctacgtt agcttcaatg attggaatat
     8341 ttttttgtcg ttgattgtga agttgattga ttacggcact tttcaaccca ctaaaactaa
     8401 aatcataact atctttatcc aaccaaacac gagggaatga ataagtatct tcaccttcag
     8461 cagccaaccg atcaacttgt ggaccacctg gataatttaa accaattgtt cgtgccactt
     8521 tatcataagc ctcacctact gcgtcatctc gtgtttcacc aatgacttca aatgataaat
     8581 gatccttcat ataaactaat tcagtatgtc cacctgaaac aataagtgca attagcggga
     8641 atgttaatgg ctcttctatg tgattagcat atatatgtcc tgcaatatga tgaacaggaa
     8701 taagtggctt atcgtaagca aatgccaatg ctttggctgc attaacacct attagtaacg
     8761 caccaattag tccagggcct tctgtaaccg ctatggcatc aatatcttct attgatacat
     8821 cggcatcccc tagagcctcg tttattgttg ctgttatacc ttcaacgtga tgtctacttg
     8881 ccacttcggg aacgacaccg ccaaatcgtt tatgactttc aatctgactt aaaactgtat
     8941 ttgataaaat atctctgcca ttttttataa cactaacgct tgtttcatca caacttgttt
     9001 caacagctag tattaatata tctttagtca tttaaattca cccacataac cattgcgtcc
     9061 tcaccttcac cataataatt tttacgttta ccaccatatt gaaatcctaa attttcatat
     9121 acatgttgtg ccactttatt attaactctt acttctaaac tcatcacatc acaagtgtga
     9181 cttgcatagt ttattccgta ttttaaaagc atttgaccta aaccatagcc tctataatta
     9241 tcatcgattg caactgttgt aatttgagct tgatcgataa caatccataa acctaaataa
     9301 ccaataattt gttgttcaaa ttctaagaca aaatatttcg caaagttatt ttgctctatt
     9361 tcatgataaa atgcgtcaat tgtccaagaa ctgtcattga aactccgacg ctcaagatca
     9421 aagacttgtg gcacatcttc tttagtcatc tctctaatgt ttaattgttc ttttgactgt
     9481 tgatccaatt tcgttccgcc tcagctaatt tatggtattt aggagtaaat gtatgtacgt
     9541 ctgaaggttt atctagcaat tgatacatga ctgatgcatt tggtagctgc gcaatcactt
     9601 caccttgtaa ttcatcttgt aattttacag tatctttccc aatataaata aatggttggt
     9661 ttaaatcttc taaaaaagct cgcaatgcct ctatcgacat atattgatct tctaaaatag
     9721 tcactaattg accattttgc cactggaata tgcctgtata aactgcttgt cgtcttgcat
     9781 caaacacagg aaccaataat ttatcagtat gatcgattgt tgctgccaat gcctttaatg
     9841 atgaaacacc atataattta acatctaacg catacgctaa tgttttagca acagtaacac
     9901 cgatacgtaa gccagtatat gaaccaggac cttcagcaac aataatcgca tctaattgct
     9961 gtttagctat tttgctctgc tcaaatagct gactaatcgc aggcattaat tgcactgaat
    10021 gattttgttt agaatccgta gttatttcag ctaaaacttc atcgttttgc atcaatgcta
    10081 ctgataatgg ttgattcgat gtatcaatga gcagcgaatt catggataat tgcctcctta
    10141 atttgttcat aatgttctcc ttgcgcgaac aactcaattt gtcttgtatt ttcagatatt
    10201 gttgaaatgt taatagataa atgcgtcgct ggaagtaaat cttttataaa ttgactccat
    10261 tcaataacag taattgcctg atcttcgaaa aattcatcaa atcctaaatc ttcatcagaa
    10321 tcttctaagc gataacaatc catatgatgc aattttaaat ttttacccct atatgattta
    10381 atgatgttaa atgtcgggga attaatcgta cgtcttacac caagagcttt tcctataaat
    10441 tgcgttaacg ttgttttacc tgctcctaaa tctccgttaa gtaaaatcaa atcaccactt
    10501 ttcaattgct caactaaaaa tatagcaaat tgattcattt catctaaatt atttatcttt
    10561 atcaatgttg attctcctat attatgcttt tcattcataa aaatgattat ccattgttca
    10621 atcgtatcta actttatatt taacctttat attgtaacaa atttcaactt aaatttctta
    10681 tctttgaaac agattatcta ttcaaagtta attgtaagaa aatttaaaat atttgttgac
    10741 atactaaagc agatatagta aattaaattt atcaaatttt tagacaattc taactattaa
    10801 agtgatatat accattcacg gaaggagtat aataaaatgc ttaatcaata tactgaacat
    10861 caaccgacaa cttcaaatat tattatttta ttatactctt taggactcga acgttagtaa
    10921 atatttacta aacgctttaa gtcctatttc tgtttgaatg ggacttgtaa acgtcccaat
    10981 aatattggga cgttttttta tgttttatct ttcaattact tatttttatt actataaaac
    11041 atgattaatc attaaaattt acgggggaat ttactatgcg aagcgacatg atcaaaaaag
    11101 gagatcacca agcaccagca agaagtcttt tacatgccac gggcgcgcta aaaagtccaa
    11161 ctgatatgaa caaaccattt gtagctattt gtaactctta tattgatatt gttcctggac
    11221 atgttcactt gagagagctt gcagatatag ctaaagaagc aattagagaa gccggtgcca
    11281 ttccatttga attcaataca attggtgttg atgatggaat agctatggga catatcggaa
    11341 tgcgatattc tctaccatca cgtgaaatta ttgcagatgc agctgaaact gtaattaacg
    11401 ctcattggtt tgacggcgta ttttacattc ctaattgtga caagattaca cccggtatga
    11461 ttttagcagc catgaggaca aacgtaccag ctatcttttg ctctggtgga ccaatgaaag
    11521 ctggcttatc tgcacatgga aaagcattaa cactttcatc aatgtttgaa gcagtcggcg
    11581 catttaaaga aggatcgatt tctaaagaag aatttttaga tatggaacaa aatgcctgcc
    11641 ctacttgtgg ttcatgtgct gggatgttta ctgcaaattc aatgaactgt ttgatggaag
    11701 ttttaggtct aacattacca tacaacggta ctgcacttgc agtcagtgat cagcgacgag
    11761 aaatgattcg ccaagcagct tttaaattag ttgaaaatat taaaaatgat ttaaaaccac
    11821 gtgatatcgt tactcgcgaa gcaattgatg atgcatttgc acttgatatg gctatgggtg
    11881 gttcaacaaa cacggtactg catacgttag ccattgccaa tgaagctggt attgattatg
    11941 acttagagcg cattaatgct attgccaaac gcacgccata tttatcaaaa atagcaccta
    12001 gttcatcgta ttcaatgcat gatgtgcatg aagctggtgg cgtcccagca attattaatg
    12061 aattgatgaa gaaagatggc acgttacacc cagatagaat cacagttact ggcaaaacgt
    12121 tacgtgaaaa taacgaaggc aaagaaatta agaactttga tgtcattcac cctcttgatg
    12181 caccatatga tgcacaaggc ggtttatcta tcttatttgg taatatcgcc cctaaaggcg
    12241 cagttattaa agttggcggc gttgatccat ctatcaaaac atttactggg aaagcaattt
    12301 gtttcaattc gcatgatgaa gctgttgaag caatagacaa tcgtaccgtt cgtgcaggcc
    12361 acgtcgttgt cattagatat gaaggaccta aaggtggacc aggtatgcct gaaatgttag
    12421 cacctacttc ctctattgtt ggtcgcggct taggtaaaga tgttgcatta attactgatg
    12481 ggcgtttttc cggtgccaca agaggtattg cagttggtca tatttcccct gaagctgcat
    12541 ctggtggacc aattgcctta attgaagatg gtgatgagat tactattgat ttaacaaatc
    12601 gtacattaaa cgtaaaccag cctgaagatg ttctagcgcg tcgccgagaa tctttaacac
    12661 catttaaagc gaaagtaaaa acaggttatc tagctcgtta tactgcccta gtaactagcg
    12721 caaatacagg tggcgtcatg caagtccctg agaatttaat ttaatttatt tttatattgg
    12781 agatggttaa aatgtctaaa actcaacatg aagtaaacca aaatattgac cctttaaaaa
    12841 tggctgaatc acttgaacct gaacaactaa atgaaaaaac tttaaatgat atgcgttcag
    12901 gatcagaagt gctagtagaa gctctactta aagaaaatgt ggattattta ttcggttatc
    12961 ctggtggtgc cgtactacct ttatatgaca cgttttatga tggtaaaatc aaacatattt
    13021 tagcaagaca cgaacaaggt gctgttcatg ctgcagaagg ttatgcacgt gtatctggta
    13081 aaactggcgt cgttgtagtt acaagcggtc caggtgcaac taatgtaatg acaggtatta
    13141 cggatgcaca ttgcgactct ttacctctag ttgtattcac tggacaagtt gctacaccag
    13201 gcattggtaa agatgcattc caagaagcgg atattctatc tatgacttca ccaattacaa
    13261 aacaaaatta tcaagtgaaa cgtgttgaag atatccctaa aatcgtacac gaagctttcc
    13321 atgtagctaa ttctggacgc aaaggtcctg tagtgattga ttttccaaaa gatatgggtg
    13381 ttttagctac aaatgtggat ttatgcgacg aaatcaatat tccaggttat gaagttgtta
    13441 cagaaccaga aaataaagac attgacactt tcatctcact tttaaaagaa gcgaaaaagc
    13501 ctgtcgtatt agccggcgca ggtattaatc aatcaaaatc aaatcaatta ttaacacagt
    13561 ttgttaataa acatcaaatc ccaacagtta ctactttact aggtctaggt gctgtacctt
    13621 atgaggatac actattttta ggtatgggag gaatgcatgg ttcttatgct agtaacatgg
    13681 cattaactga gtgtgattta ctcattaatt taggtagccg cttcgatgat agattagcaa
    13741 gcaaacctga tgcctttgca cctaacgcca aaattgtaca tgtagatatt gatccttcag
    13801 aaatcaataa agttattcat gtagatttag gtattattgc agactgtaaa agatttttag
    13861 aatgtttaaa tgataaaaat gttgagacta tagaacacag tgactgggtt aaacattgtc
    13921 aaaataataa gcagaaacac ccatttaaac ttggtgaaga agatcaagta ttttgtaagc
    13981 cacaacaaac aatcgaatat atcggcaaaa ttacaaatgg tgaagcaatt gttactacag
    14041 acgtgggaca acatcaaatg tgggcagctc aattttatcc atttaaaaat cacggacaat
    14101 gggttacaag cggtggttta ggaacaatgg gattcggtat tccttcgtca attggtgcca
    14161 aattagctaa tcctgataaa acagtcgtat gtttcgtcgg tgacggtggt ttccaaatga
    14221 caaaccaaga aatggcactt ttacccgaat atggtttaga tgtcaaaatc gtactaatca
    14281 ataatggaac attaggtatg gttaaacaat ggcaagataa gttctttaat caacgcttct
    14341 cacactcagt atttaatggt caacctgatt ttatgaaaat ggcagaagca tatggcgtca
    14401 aaggtttctt aatcgataag ccagaacaac tggaagaaca attagatgca gcgtttgctt
    14461 atcaaggacc agctttaatt gaggttcgta tttcccctac tgaagctgta accccaatgg
    14521 ttccgagtgg caaatcaaat catgaaatgg agggcttata atgacaagaa ttcttaaatt
    14581 acaagttgcg gatcaagtca gcacgctaaa tcgaattaca agtgcttttg ttcgcctaca
    14641 atataatatc gatacattac atgttacaca ttctgaacaa cctgggattt ctaacatgga
    14701 aattcaagtc gatattcaag atgatacatc acttcatata ttaattaaaa aattaaaaca
    14761 acaaattaat gttttaacgg ttgaatgcta cgaccttgtt gataacgaag cttaatttta
    14821 agacaaaggc aatgatgcgc taattagtta tagatatatc ataggctgct agttaacatc
    14881 tgccactatt acaaagttat atttcagaat tttcgaaaca caaaatattt aattatttgg
    14941 aggaatttat tatgacaaca gtttattatg atcaagatgt aaaaacggac gctttacaag
    15001 gcaaaaaaat tgcagtagta ggttatggat cacaaggtca cgcgcatgca caaaacttaa
    15061 aagacaatgg atatgatgta gtcatcggca ttcgcccagg tcgttctttt gacaaagcta
    15121 aagaagatgg atttgatgtg ttccctgttg cagaagcagt taagcaagct gatgtaatta
    15181 tggtgctatt acctgatgaa attcaaggtg atgtatacaa aaacgaaatt gaaccaaatt
    15241 tagaaaaaca taatgcgctt gcatttgctc atggctttaa cattcatttt ggtgttattc
    15301 aaccaccagc tgatgttgat gtatttttag tagctcctaa aggaccgggt catttagtta
    15361 gacgtacatt tgttgaaggt tctgctgtac catcactatt tggtattcaa caaggcgctt
    15421 caggtcaagc acgtaatatt gctttaagtt atgcaaaagg tattggtgca actcgtgcag
    15481 gtgttattga aacaacattt aaagaagaaa ctgagacaga tttatttggt gaacaagcag
    15541 tactttgcgg tggtgtatcg aaattaattc aaagtggctt tgaaacatta gtagaagcgg
    15601 gttatcaacc agaattagct tattttgaag tattacatga aatgaaatta atcgttgatt
    15661 tgatgtatga aggcggtatg gaaaatgtac gttactcaat ttcaaatact gctgaatttg
    15721 gtgactatgt ttcaggacca cgtgttatca caccagatgt taaagaaaat atgaaagctg
    15781 tattaactga tatccaaaat ggtaacttca gtaatcgctt tatcgaagac aataaaaatg
    15841 gattcaaaga attttataaa ttacgcgaag aacaacatgg tcatcaaatt gaaaaagttg
    15901 gtcgtgaatt acgcgaaatg atgcctttta ttaaatctaa aagcattgaa aaataagata
    15961 gacctacaat gaggagttgt taaatatgag tagtcatatt caaatttttg atacgacact
    16021 aagagacggt gaacaaacac caggagtgaa ttttactttt gatgaacgct tgcgtattgc
    16081 attgcaatta gaaaaatggg gtgtagatgt tattgaagct ggatttcctg cttcaagtac
    16141 aggtagcttt aaatctgttc aagcaattgc acaaacatta acaacaacgg ctgtatgtgg
    16201 tttagctaga tgtaaaaaat ctgacatcga tgctgtatat gaagcaacaa aagatgcagc
    16261 gaagccggtc gtgcatgttt ttatagcaac atcacctatt catcttgaac ataaacttaa
    16321 aatgtctcaa gaagacgttt tagcatctat taaagaacat gtcacatacg cgaaacaatt
    16381 atttgacgtt gttcaatttt cacctgaaga tgcaacgcgt actgaattac cattcttagt
    16441 gaaatgtgta caaactgccg ttgacgctgg agctacagtt attaatattc ctgatacagt
    16501 cggctacagt taccatgatg aatatgcaca tattttcaaa accttaacag aatctgtaac
    16561 atcttcaaat gaaattattt atagtgctca ttgccatgac gatttaggaa tggctgtttc
    16621 aaatagttta gctgcaattg aaggcggtgc gagacgaatt gaaggcactg taaatggtat
    16681 tggtgaacga gcaggtaatg cagcacttga agaagtcgcg cttgcactat acgttcgaaa
    16741 tgatcattat ggtgctcaaa ctgctcttaa tctcgaagaa actaaaaaaa catcggattt
    16801 aatttcaaga tatgcaggta ttcgagtgcc tagaaataaa gcaattgttg gccaaaatgc
    16861 atttagtcat gaatcaggta ttcaccaaga tggcgtatta aaacatcgtg aaacatatga
    16921 aattatgaca cctcaacttg ttggtgtaag cacgactgaa cttccattag gaaaattatc
    16981 tggtaaacac gccttctcag agaagttaaa agcattaggt tatgacattg ataaagaagc
    17041 gcaaatagat ttatttaaac aattcaaggc cattgcggac aaaaagaaat ctgtttcaga
    17101 tagagatatt catgcgatta ttcaaggttc tgagcatgag catcaagcac tttataaatt
    17161 ggaaacactg caactacaat atgtctctag cggccttcaa agtgctgttg ttgttgttaa
    17221 agataaagag ggtcatattt accaggattc aagtattggt actggttcaa tcgtagcaat
    17281 ttacaatgca gttgatcgta ttttccagaa agaaacagaa ttaattgatt atcgtattaa
    17341 ttctgtcact gaaggtactg atgcccaagc agaagtacat gtaaatttat tgattgaagg
    17401 taagactgtc aatggctttg gtattgatca tgatatttta caagcctctt gtaaagcata
    17461 cgtagaagca catgctaaat ttgcagctga aaatgttgag aaggtaggta attaattatg
    17521 acttataaca ttgttgccct acctggtgat ggaatcggtc cagaaatttt gaacggatct
    17581 ctatcattgc ttgaaattat aagtaataaa tataacttta attatcaaat agagcaccac
    17641 gaatttggtg gtgcctctat tgatacattc ggcgagcctt taactgagaa aaccttaaat
    17701 gcgtgtaaaa gagcagatgc tattttactg ggtgcaatcg gtggacctaa atggacagat
    17761 cctaacaatc gaccagaaca aggattatta aaattgcgta aatccttaaa tttatttgta
    17821 aatatacgcc ccactaccgt tgtcaaaggc gctagttctt tatcaccttt aaaggaagaa
    17881 cgcgttgaag gcacagattt agttatagtc cgtgaattga caagtggtat ttattttgga
    17941 gaacctagac attttaataa tcacgaggcc ttagattctc ttacttatac aagagaagaa
    18001 atagaacgca ttgttcacgt agcatttaaa ttggccgctt caagacgagg aaaactaaca
    18061 tcagttgata aagaaaatgt attagcttct agtaaattgt ggcgcaaagt cgtaaatgaa
    18121 gtaagtcaat tatatccaga agtaacagta aatcacttac ttgttgatgc ttgtagtatg
    18181 catttaatca caaatccaaa acaatttgac gtcatcgtat gtgaaaactt atttggcgat
    18241 attttaagtg atgaagcttc agtgattcct ggttcacttg gtttatcacc ttctgctagt
    18301 tttagtaacg atggtccaag attgtatgag cctattcatg gatcagcacc agatattgca
    18361 ggtaaaaacg ttgccaatcc atttggaatg attctatctt tagcgatgtg tttacgtgaa
    18421 agcttaaatc aaccagatgc tgcagatgaa ttagaacaac atatttatag catgattgaa
    18481 catgggcaaa cgacagcaga tttaggcggc aaattgaata ctactgatat tttcgaaatt
    18541 ctatctcaaa aattgaatca ctaaggggga gatgtaaatg ggtcaaacat tatttgacaa
    18601 ggtgtggaac agacatgtgt tatacgggaa attgggcgaa ccgcaactat tatacattga
    18661 tttacacctt atacatgaag ttacttctcc tcaagcattt gaaggactta ggcttcaaaa
    18721 cagaaaatta agacgcccag atttaacatt tgcaacactc gatcacaatg ttcctactat
    18781 tgatatattc aatattaaag atgaaattgc aaacaaacaa atcacaacat tacaaaaaaa
    18841 cgccatagat tttggggtgc atatttttga tatgggttct gatgaacaag gtattgttca
    18901 catggtagga cctgagacag gacttacaca gcctggcaag acaatcgttt gtggtgactc
    18961 tcacacagca acacatggag catttggtgc tattgcattt ggaattggaa caagtgaagt
    19021 tgaacatgtt ttcgcaactc aaacgctatg gcaaacaaaa cccaaaaact taaaaatcga
    19081 tattaatggt accttaccaa caggcgtcta tgctaaggac attattctgc atttaattaa
    19141 aacgtatggt gttgactttg gtacaggcta tgctttggaa tttactggcg aaacaattaa
    19201 aaacctttca atggatggtc gaatgactat ttgtaacatg gctatcgaag gtggtgccaa
    19261 atacggcata atccaacctg atgatataac atttgaatat gttaaaggga gaccatttgc
    19321 cgataacttc gctaaatcag ttgataagtg gcgtgagcta tattctgatg acgacgcgat
    19381 atttgatcgt gtaattgaac ttgatgtttc aacattagaa ccacaagtga catggggaac
    19441 taatcctgaa atgggtgtta atttcagtga accattccct gaaatcaatg atatcaacga
    19501 tcaacgtgcg tatgattata tggggttaga accaggtcaa aaagctgaag acatcgactt
    19561 agggtatgtt tttctcggtt catgtacaaa tgctagacta tcagatttga ttgaagctag
    19621 tcatattgtt aaaggaaata aagttcatcc aaatattaca gctattgtcg taccaggttc
    19681 tcgtacagta aaaaaagaag cagaaaaatt aggtctagat actatcttta aaaatgcagg
    19741 atttgaatgg cgtgaaccag gatgttcaat gtgtttaggc atgaatcctg accaagtacc
    19801 tgagggcgta cattgtgcat ctacaagtaa tcgaaacttt gaaggacgac aaggcaaagg
    19861 tgcaagaaca catttagtat cccctgctat ggcagcagca gcagctattc atggtaaatt
    19921 tgtggacgta agaaaggtgg ttgtttaaat ggcagcaatc aaacctatta caacatataa
    19981 aggtaaaata gtccctctct tcaacgacaa tatcgataca gaccaaatca ttcctaaggt
    20041 acacttaaag cgtatttcaa aaagtggctt tggtccattt gcttttgatg aatggcggta
    20101 cttacctgat ggttcagata atcctgattt caatcctaac aaaccacaat ataaaggggc
    20161 ttctatttta attactggag ataattttgg atgtggttca agtcgtgaac atgctgcttg
    20221 ggctcttaag gactatggtt ttcatattat tattgcagga agtttcagtg acatatttta
    20281 tatgaattgc actaaaaatg cgatgttgcc tatcgtttta gaaaaaagtg cccgtgaaca
    20341 tcttgcacaa tatgttgaaa ttgaggtcga tttaccaaat caaactgtgt catcaccaga
    20401 caagcgtttc cattttgaaa ttgatgaaac ttggaagaat aaacttgtaa atggcttaga
    20461 tgacattgca atcaccctac aatatgaatc attaatagaa aaatatgaaa aatcacttta
    20521 agggagttga atattatgac agtcaaaaca acagtttcta cgaaagatat cgatgaggca
    20581 tttttaagac ttaaagatat tgtcaaagaa acacctttac aattagacca ttacttatct
    20641 caaaagtatg attgtaaagt ctatttaaaa cgagaagatt tacaatgggt acgttctttt
    20701 aaattaagag gtgcttacaa cgctatttct gttttatcag atgaagctaa aagtaaaggt
    20761 attacatgtg caagtgcagg taatcatgct caaggtgttg cctatacagc taaaaaactt
    20821 aatttaaacg ctgttatctt tatgccagtc actacacctt tacaaaaggt aaatcaagta
    20881 aagttctttg gaaatagtaa cgttgaagtt gtactcactg gtgatacatt tgatcactgt
    20941 ttagctgaag ctttaactta tacaagtgaa catcaaatga actttataga tccattcaat
    21001 aatgttcata caatttctgg acaaggtacg cttgctaaag aaatgctaga acaagcaaag
    21061 tctgacaatg ttaactttga ttatctattt gccgcaattg gtggtggcgg tttaatttca
    21121 ggtattagta cttactttaa aacctattca cctaccacga aaattatagg tgttgaacct
    21181 tcaggtgcaa gtagtatgta tgaatctgtt gtggtaaata atcaggtagt cacattgcct
    21241 aatatcgata aatttgtgga cggtgcatct gtagctagag ttggcgatat tacatttgaa
    21301 attgcaaaag aaaatgtaga tgattacgtt caagtagatg aaggtgcagt ttgttctacg
    21361 attttagata tgtattcaaa acaagcaatt gtagcagaac ctgctggcgc attaagtgta
    21421 agtgcgcttg aaaactataa agatcatatt aaaggtaaaa cagtggtttg tgtcattagt
    21481 ggtggtaata atgatattaa tcgaatgaaa gaaattgaag aacgttcatt actatacgaa
    21541 gaaatgaagc attactttat cttaaatttc cctcaacgtc caggtgcatt gagagaattt
    21601 gtaaatgacg tattaggacc tcaagacgat attactaaat ttgaatactt aaaaaaatct
    21661 tctcaaaata caggtactgt cattattggt attcaactta aagatcatga tgatttaata
    21721 caactcaaac aac
//
LOCUS       DAKTIO010000026        21688 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.026, whole genome shotgun sequence.
ACCESSION   DAKTIO010000026 DAKTIO010000000
VERSION     DAKTIO010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21688
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.026"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            5..448
                     /locus_tag="O0293_000752"
                     /pseudo
     CDS             5..448
                     /locus_tag="O0293_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501131.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
     gene            668..1096
                     /locus_tag="O0293_000753"
     CDS             668..1096
                     /locus_tag="O0293_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HCY0279649.1"
                     /translation="MILNFNQFENQNFFNANPSDTFKDLGKQVFNYFSTPSFVTNIYE
                     TDELYYLEAELAGVNKEDISIDFNNNTLTIQATRSAKYKSEQLILDERNFESLMRQFD
                     FEAVDKQHITASFENGLLTITLPKIKPSNETTSSTSIPIS"
     gene            complement(1203..2372)
                     /locus_tag="O0293_000754"
     CDS             complement(1203..2372)
                     /locus_tag="O0293_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NarK/NasA family nitrate transporter"
                     /protein_id="HCY0279650.1"
                     /translation="MYKTKGGFQLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQ
                     ISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSFIVLLFPIFFLSQAQTPGMLMAS
                     GFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQ
                     TTVRSYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGA
                     FVAFGIFLPNYLVNHFGIDKVDAGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDF
                     VVMIIGAIILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSYFLNEAGSANGIVSM
                     MGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKEYGSLKNG"
     gene            2742..3380
                     /locus_tag="O0293_000755"
     CDS             2742..3380
                     /locus_tag="O0293_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HCY0279651.1"
                     /translation="MYKINIIRSDSVYNNILKAPINDRDSIFTKEILVPFKKKFEVQH
                     MPIYNDDKQTMSAIQFLDAFQISPKDLRMSDQMSIQYLNNDFWSNCEKYLKVAIDQFS
                     NYSISSQVSNYHFTVLLGDRQKPLMYLNKNRGGDGGIPGYIMIYLVPSTSTINSMKSL
                     IAHEVNHNMRYQYIDWDGGSLIELIIAEGLAENYVESLYGKAHIGPWVTNTN"
     gene            3399..3656
                     /locus_tag="O0293_000756"
     CDS             3399..3656
                     /locus_tag="O0293_000756"
                     /inference="COORDINATES: protein motif:HMM:NF021537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279652.1"
                     /translation="MKNTIYNHLHLKHIFESMPYLYGDDINKLQGRPIVGLSHAAGYA
                     CGYHLVKYFLQKTNIPIEVATTLPAQKIINEVTEFWHTHTL"
     gene            3635..4375
                     /locus_tag="O0293_000757"
     CDS             3635..4375
                     /locus_tag="O0293_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCY0279653.1"
                     /translation="MAYTYTLKDIIEITGVTKRTLHYYDEIGLLVPDKNDKNYRVYKQ
                     QDLEKLQKILILKSFDFDIAKIKQYISYDNEQLRKLLSEQISKLDKKISDLQLIRRSV
                     CEFIKGLSLIDTSILNKTLQSQYDKEASIKYGHTKAYQSFIRRKDSLQSQDIRHKLTT
                     IFNKFNHMSLSHYPIQDCSDLVFEWKAFMNTIADFDDETLCCIAKTYEDDTRFKDYFN
                     SYDNQNLASYISEAVNYFLSNVNKSDNF"
     gene            complement(4633..5286)
                     /gene="nreC"
                     /locus_tag="O0293_000758"
     CDS             complement(4633..5286)
                     /gene="nreC"
                     /locus_tag="O0293_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002464689.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation response
                     regulator NreC"
                     /protein_id="HCY0279654.1"
                     /translation="MKIVIADDHAVVRTGFSMILNYQNDMEVVATAADGVEAYQKVME
                     YKPDVLLMDLSMPPGESGLIATSKIADSFPETKILILTMFDDEEYLFHVLRNGAKGYI
                     LKNAPDEQLLLAIRTVYKGETYVDMKLTTSLVNEFVSNSNQDTANTTDPFKILSKREL
                     EILPLIAKGYGNKEIAEKLFVSVKTVEAHKTHIMTKLGLKSKPELVEYALKKKLLEF"
     gene            complement(5309..6343)
                     /gene="nreB"
                     /locus_tag="O0293_000759"
     CDS             complement(5309..6343)
                     /gene="nreB"
                     /locus_tag="O0293_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501138.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation sensor histidine
                     kinase NreB"
                     /protein_id="HCY0279655.1"
                     /translation="MINEDSIQLDTLLKKYYEHSIEKIVFADDNGKIIAMNDAAKDIL
                     SEEDNYSAVANAICHRCEGYTNAYDVQSCKDCFLESMQVQATNFQVFMKTKDQKVMPF
                     TATYQLIDQDRGIHAFTLQNVSSQIEQQEKLHQQRMMRKTISAQENERKRISRELHDS
                     VIQEMLNVDVQLRLLKYQEDTTKLLEDAENIEYIVAKLIDDIRNMSVELRPASLDDLG
                     LEAAFKSYFKQFEENYGIKIIYTSNIKNTRFDSDIETVVYRVVQEAILNALKYADVNE
                     INVGIRQTGRHLVAEVIDAGNGFDPSSKPKGSGLGLYGMNERAELVSGSVNIETKIGE
                     GTNVTLNIPI"
     gene            complement(6367..6819)
                     /gene="nreA"
                     /locus_tag="O0293_000760"
     CDS             complement(6367..6819)
                     /gene="nreA"
                     /locus_tag="O0293_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation accessory nitrate
                     sensor NreA"
                     /protein_id="HCY0279656.1"
                     /translation="MTPEAIIEDRRFQETLDKIRKEEGYDFAAIAFYESNKPSSPIKW
                     HYVSGNKNNRFKLIILRKGRGLAGTVMKTGKRMVIANVGLALGPEENIDYPILLSESL
                     TAVLAVPLWYKNQVYGVLLFGQRDGRPLPKIFDNDDIQRKFGIFNDDK"
     gene            complement(6839..7516)
                     /gene="narI"
                     /locus_tag="O0293_000761"
     CDS             complement(6839..7516)
                     /gene="narI"
                     /locus_tag="O0293_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCY0279657.1"
                     /translation="MLNQFLWVIFPYLCLVVFVAGHIARYRYDKFSWTAKSSELIERK
                     RLMWGSLLFHLGIIPVFFGHVVGLLIPKPWMDAVGVSEHLYHIGAVYIGSIFGIITLI
                     GMFLLTARRVTTKSIRRLSSASDIFVNFLLLLIVFMGCYATLVTNIQHPDFNYRTSLS
                     IWFRQLFMLKPDASLMSGVPIAFKMHILLGFTIMACWPFTRLVHVWSVPLTYINRRYI
                     IYRKNKV"
     gene            complement(7509..8084)
                     /gene="narJ"
                     /locus_tag="O0293_000762"
     CDS             complement(7509..8084)
                     /gene="narJ"
                     /locus_tag="O0293_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCY0279658.1"
                     /translation="MINFDNFKKYQESFGYMAQQLCFPEKLTFHPKTFEETISKDHPA
                     YEDLVAFRNVMMTFSLSEIKAIYTDTFDFTKKAPLYMTYNKFDTQKERGQMLAKLKVL
                     YEMFGLEMVDNELSDYLPLMLQFLQVAEWRNDPRAEENIQLIIMIIEDGTYVMANALG
                     EDNNPYAYVIQALRKTLKLCLTSPKGVKHHA"
     gene            complement(8077..9636)
                     /gene="narH"
                     /locus_tag="O0293_000763"
     CDS             complement(8077..9636)
                     /gene="narH"
                     /locus_tag="O0293_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCY0279659.1"
                     /translation="MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNV
                     ETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPLIKDY
                     YEPWNYNYEHLTTAKSGKHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPN
                     IQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACR
                     GWRYCMTGCPYKKVYFNWKTNKAEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYD
                     ADRVHEAASAVDEKDLYEKQLDIFLNPFDEEVIAQAEKDGIGYDWIEAAQNSPIYKLA
                     IEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYL
                     ANIFTAGDTEPVKGALQRMAMMRSYMRSQVTQQPFDTSRLERLGITERQTKDMYRLLG
                     LAKYEDRFVIPTSHKETYLDTYHAQGSTGYNYGGEHFGDNCEGCGVAVGSGKTGQEIY
                     NENFYGGIFRD"
     gene            complement(9626..13315)
                     /locus_tag="O0293_000764"
     CDS             complement(9626..13315)
                     /locus_tag="O0293_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCY0279660.1"
                     /translation="MGKFGLNFFKPTEKFNGNWSILESKSREWEKMYRERWSHDKEVR
                     TTHGVNCTGSCSWKVFVKNGVITWENQQTDYPSCGPDMPEYEPRGCPRGASFSWYEYS
                     PLRIKYPYIRGKLWDLWTEALEENNGNRVAAWASIVENEDKAKQYKQARGMGGHVRSN
                     WKDVTEIIAAQLLYTIKKYGPDRIAGFTPIPAMSMISYAAGARFINLLGGEMLSFYDW
                     YADLPPASPQIWGEQTDVPESSDWYNASYIIMWGSNVPLTRTPDAHFMTEVRYKGTKV
                     ISVAPDYAENVKFADNWLAPNPGSDAAIAQAMTHVILQEHYVNQPNERFINYAKQYTD
                     MPFLIMLDEDENGYKAGRFLRASDLGQTTEQGEWKPVIHDAISDSLVVPNGTMGQRWE
                     EGKKWNLKLETEDGSKINPTLSMTEGGYELETIQFPYFDSDGDGIFNRPIPTRQVTLA
                     NGDKVRIATIFDLMASQYGVRRFDHKLESKGYDDAESKYTPAWQEAISGVKQSVVIQV
                     AKEFAQNAIDTEGRSMIIMGAGINHWFNSDTIYRSILNLVMLCGCQGVNGGGWAHYVG
                     QEKCRPIEGWSTVAFAKDWQGPPRLQNGTSWFYFATDQWKYEESNVDRLKSPLAKTEE
                     LKHQHPADYNVLAARLGWLPSYPQFNKNSLLFAEEAKDEGIDSNEAILQRAIDEVKSK
                     QTQFAIEDPDLKKNHPKSLFIWRSNLISSSAKGQEYFMKHLLGTKSGLLATPNEDEKP
                     EEITWREETTGKLDLVVSLDFRMTATPLYSDIVLPAATWYEKHDLSSTDMHPYVHPFN
                     PAIDPLWESRSDWDIYKTLAKAFSEMAKDYLPGTFKDVVTTPLSHDTKQEISTPYGVV
                     KDWSKGEIEAVPGRTMPNFAIVERDYTKIYDKYVTLGPVLEKGKVGAHGVSFGVSEQY
                     EELKSMLGTWSDTNDDSVRANRPRIDTARNVADAILSISSATNGKLSQKSYEDLEEQT
                     GMPLKDISSERAAEKISFLNITSQPREVIPTAVFPGSNKQGRRYSPFTTNIERLVPFR
                     TLTGRQSYYVDHEVFQQFGESLPVYKPTLPPMVFGNRDKKIKGGTDALVLRYLTPHGK
                     WNIHSMYQDNKHMLTLFRGGPTVWISNEDAEKHDIQDNDWLEVYNRNGVVTARAVISH
                     RMPKGTMFMYHAQDKHIQTPGSEITDTRGGSHNAPTRIHLKPTQLVGGYAQISYHFNY
                     YGPIGNQRDLYVAVRKMKEVNWLED"
     gene            complement(13624..14601)
                     /gene="cobA"
                     /locus_tag="O0293_000765"
     CDS             complement(13624..14601)
                     /gene="cobA"
                     /locus_tag="O0293_000765"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCY0279661.1"
                     /translation="MSVEEYGKVYLIGAGPGNPNYLTKKAERLIREADVILYDRLVNP
                     LILQYANLTTEIIDVGKKPYAKHIQQEKINDCIVEAARRYNKVVRLKGGDPAIFGRVQ
                     EEVDTLNEHHIAFEIVPGVTSASAAVATMQTGLTMRTVAKSVTFSTGHFKDSEENEVD
                     VNSLVNGGTLAIYMGVKRLGKIITQIQQYTDIDYPIAIVFQASCFNEFVVKGRLSNII
                     SKLQHYSIEAKPGICIIGEVVDYTENTPKSYDPMKQFYVVSGSKHDALMLCEHLYDEG
                     YGCLLNPNDTSNGTYHSSQYDYYDAFIKQQENVTYISTDRADANTVLCH"
     gene            complement(14592..14906)
                     /gene="nirD"
                     /locus_tag="O0293_000766"
     CDS             complement(14592..14906)
                     /gene="nirD"
                     /locus_tag="O0293_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCY0279662.1"
                     /translation="METKEKIKVTTIDELTPLIGKKVIVKGKEVGLFLTESGKIHAIH
                     NICPHKQGPLSEGTVSGEYVFCPLHDQKIDLNTGIVQEPDEGCVDVYEVEVTDGNVYI
                     CL"
     gene            complement(14910..17315)
                     /locus_tag="O0293_000767"
     CDS             complement(14910..17315)
                     /locus_tag="O0293_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0279663.1"
                     /translation="MAKQKLVMIGNGMAGIRTIEEILERANDLYDITVIGKEPYPNYN
                     RIMLSNILQNKMTVEETIMNPYEWYEEHGIELITNDPVIEVDRANQSVTTANGIEVSY
                     DKLIFATGSKAFVIPVPGSTLPSVIGWRTLDDTEQMMNIAKTKKKAIVIGGGLLGLEC
                     ARGLLDQGMEVTVLHLAEWLMEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDD
                     VEGVKLADGREIPADLVVMAVGIRPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVG
                     ECAEHNGKVYGLVAPLYEQGKVLADHLTNKETNGYKGSTTFTSLKVSGCDLYSAGQIV
                     ENAEIKGIEIFNSVDNNYKKIFLKDGNVVGAVLYGDIDDGSRFYNMMKKGESTEDYTL
                     VSLLTKGGEEASLSIADMADDETICGCNGVDKGTIVNAITENGFTTVEEVTAKTKAGN
                     SCGKCKPQIAQILQHTLGDDFVAAKPAGICGCTDLTRDQIVTQIRAKGLKTSKEVRHV
                     LNFKNKGGCPKCRPAINYYLNMVYPHDHEDERESRFANERYHANIQNDGTFSVIPQMR
                     GGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGLYGVKKEELPNIWEDLGMRSASAYG
                     KKTRSVKSCVGKEFCRFGTQYTTRLGIRLEKTFEYIDTPHKFKMGVSGCPRSCVESGV
                     KDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDEVIKLCGALMQYYRETGIYAE
                     RTAPWLRRLGFENVKEVLLDPERQNELFERIMDAKKAVEAEPWEAITSNAQARRIFEV
                     EKV"
     gene            complement(17381..18112)
                     /locus_tag="O0293_000768"
     CDS             complement(17381..18112)
                     /locus_tag="O0293_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HCY0279664.1"
                     /translation="MNGNIIVAHGMRHGRQNQALEAFISELVKDDIHHYDIAFLESEH
                     QDLETVMTTLIQSGVDHFKIVPLLIFSAMHYLKDIPNIVHEMKRRYPDIKVEVSEPLG
                     THPLMRRLIEQRICDALAGDEQQVGVMVVAHGNINGKFTKAHEELQICAQNLQISKPT
                     YARTLYGEISFTHDLESISKRYQKLIVVPLFLYDGRLVNKVKQQMNDMVINTDIHFTP
                     SINFDPILKQIINDRLESLMIPMKI"
     gene            complement(18342..18878)
                     /locus_tag="O0293_000769"
     CDS             complement(18342..18878)
                     /locus_tag="O0293_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0279665.1"
                     /translation="MTKTMIRLASEQDAEKLQQLMHEAFTPLRELGIDWPSVNANLDA
                     VKENIDKNTTFVMTIDDEIISTITVRYPWGSVKSISGYPFVWWFATNPEYEGKGYGSQ
                     LLTYVEEAFLRDTLKSAAVTLGTSARLHPWLLKIYEKRGYEIYSEHENDDGDLGVIMR
                     KVLIPERFDETILGQPPF"
     gene            complement(19111..19935)
                     /locus_tag="O0293_000770"
     CDS             complement(19111..19935)
                     /locus_tag="O0293_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCY0279666.1"
                     /translation="MIENKKTVEDTYSTGAIVDSISSSVQMKQVMVQQTPGRYMLKAM
                     MAGFLLSIVTVFMFGIKTQFASTHVDGLINLMGAIAFSLGLILVVLTNSELLTSNFMY
                     FTVGWYYKVVSVKKMTWILLYCFLGNILGGFVLFFLMKFAHVMTPEMTQALTALVQKK
                     TVDSTWLNIFTKGIFCNFFINIGIFISMQFKGGLTKAFFIACGVVVFVYMGYEHVVFN
                     AGLYAGMVFFNLDAVSWLHVLKNIVFAFLGNFVGGGIFVGLVYAFLNGKRNRLEQQ"
     gene            complement(20152..20334)
                     /locus_tag="O0293_000771"
     CDS             complement(20152..20334)
                     /locus_tag="O0293_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279667.1"
                     /translation="MVERYIKVLILYIFTTLLSSISVTSKCVPNKVIRFILRTAIGYS
                     VFAYGLHYFSNLKKNK"
     gene            complement(20418..20885)
                     /locus_tag="O0293_000772"
     CDS             complement(20418..20885)
                     /locus_tag="O0293_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HCY0279668.1"
                     /translation="MTIKVEDNKIIFSRTIEAPIEKVFDAYTTKALFEKWFHPKDAST
                     KVFRFNAVSGGDAFYAIKTPTMTSYTLAEYKTVKRPYLIEYIDSFATPQGAKDTKMPS
                     MKITLSFSKSNTTKTTVTSTSVFPTKEAAQQVINMGVEQGMNQTLDQLDALLK"
     gene            complement(21071..>21688)
                     /locus_tag="O0293_000773"
     CDS             complement(21071..>21688)
                     /locus_tag="O0293_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCY0279669.1"
                     /translation="SNIENIGKALDSGVKVKDDKAESKHDKAISDGYFKDEQVKDREL
                     SDYAGEWQSVYPYLKDGTLDEVMEHKAENDPKKSAKDLKAYYDKGYKTDITNIDIKGN
                     EITFTKDGKKHTGKYEYNGKKTLKYPKGNRGVRFMFKLVDGNDKDLPKFIQFSDHNIA
                     PKKAEHFHIFMGNDNDALLKEMDNWPTYYPSKLNKDQIKEEMLAH"
BASE COUNT         6779 a         4277 c         3129 g         7503 t
ORIGIN      
        1 ttggatgtat gtagaaaaca gctatcttag caaacagttg tgttttttat tttatgtttc
       61 ttcaaaagaa ataattaaaa aatacacaaa ctatcttaag gaatatgatt taacctatac
      121 tggttacatt gttttaatgg cgattgaaaa tgatgaaaaa cttaacatca aaaaattagg
      181 tgaacgtgtg ttcttagatt ctggaacact gacaccatta ctaaagaaat tagaaaagaa
      241 agattacgtt gttcgaacac gtgaagagaa agatgaaaga aacctacaaa tttctctaac
      301 agaacaaggt aaagcaataa aaagccctct tgctgaaatt tctgttaagg tctttaatga
      361 attcaacatt tcagaacgag aagcatcaga tataattaat aatttgcgga attttgtttc
      421 taaaaatttt gactattccg acaaaaaaat aactccctat cataaggtga tgggcagaag
      481 tatgcatcga tatgtttatt cattatcttt attgcttgta taaatctcga tgctcagttc
      541 ttttgccctg attccttatc aggcacaaat ctacccattt attacttttt tatctcccct
      601 tttactttaa cttttttaat actactcata taatggcttt gtaatacaaa tttctataat
      661 ggaagtgatg atattgaact tcaatcaatt cgagaatcaa aactttttta acgctaatcc
      721 aagtgataca tttaaagatt taggtaaaca agtatttaat tacttttcaa caccttcatt
      781 tgtaacgaat atatatgaaa cagacgaatt atattactta gaagctgaac tagcaggtgt
      841 aaataaagaa gatattagta tcgatttcaa taataatacg ctcactattc aagctactag
      901 aagcgcaaaa tacaaatctg aacaactcat tttagatgag cgtaacttcg aatcattaat
      961 gcgtcaattt gattttgaag ctgttgataa gcaacatatt actgctagtt ttgaaaatgg
     1021 gttattaacc attaccttgc ctaaaatcaa accaagcaat gaaactactt catcaacatc
     1081 tattccaatt tcatagttaa atttaaaatg aatacactac taaagcctta atgtcatgct
     1141 acgaatattg cgcatgatat taaggctttg tatttaacta aatgaatggc atgtattaca
     1201 tatcaaccgt ttttcaatga gccatattct ttttggtata aatgacgcat ggtaaataat
     1261 gcaatacatc caaataccgc taagaaaata aatgctaaat gacttgatcc tgttaaatta
     1321 gctacgtacg tgattactag tggtgggaag aatcctccta aaccacccat cattgatacg
     1381 ataccatttg cggatcccgc ttcatttaag aagtatgatg gtactaattt gaagattaag
     1441 ccgttaccaa tacctgcaca aatacttatt gttaaacagc ctacagtgaa taatgcgata
     1501 tggtctgaaa tacctaaaat aatggcaccg ataatcataa caacaaaatc aatcatcaat
     1561 actttaactg cattaaattt atcacctaaa atgccaccta ttggtcttaa gaatgttgcc
     1621 agcgcaatga atacacctga tcgaatacca gcatctactt tgtcaattcc aaaatgattt
     1681 actaagtagt taggtaagaa aataccaaat gctacaaaag caccaaaagt aataaaatac
     1741 caataactta agtaatataa tttgtagttt ttagataatg ttttcatttg agccattaat
     1801 ggtactttaa ttttacgttc ttgtgtgtca ccaaaaatga acataattaa tgcaaataaa
     1861 gcgataataa ttaagtaact tctaactgtt gtttgccaac caataatacc cgctatcggt
     1921 ggtgctaaaa atgaagaaac tgctgtaccg atatttccca taccataaat accatttgct
     1981 agacctactt tttctttagg gaaatattta ggaactgatg taacaccaac tgagaaaatt
     2041 gcaccaccta caccaaggaa aaatcctgaa gccattaaca tacccggtgt ttgtgcttgg
     2101 cttaagaaaa atatcgggaa taacaatacg ataaaactag taaagaatac ccatttagcg
     2161 ccaacaatgt ttgttaaata accaaatggc acacggagca ccgatcccaa aataactggt
     2221 atcgctaaaa tgattgatat ttgaccttca gtaacattga catcttgttt aataaagggc
     2281 attaatggcg caattatact ccaagccata aacccaacca ctaaacttaa tgtttgtaat
     2341 gtaagttgaa agccaccttt tgttttgtac atacgtttca ccttcatcaa aatcatttat
     2401 tttttaatag atatcaatac tctgcctata tattagaggt caatgttatt taaaaatgct
     2461 ttcatttata tgaaaacatt caatatagac aaagtattga ctacatatat attttattat
     2521 gattgtgaaa aagtgaacat aaggattttc ccttatttat gaagtgattt accctataca
     2581 atgtcccttt aagatgcttt tagacactta ttcgccatca ttttaatcat attatttagg
     2641 aacactcaca tacttttctt aatttttttc aattagttac aattactgct tgaaaatcac
     2701 gttacgttat cctttatgct gacaacataa ggaggtttat catgtataaa atcaatatta
     2761 ttagatcaga ttccgtctat aataatatac tcaaggcacc aataaacgac cgtgattcaa
     2821 ttttcactaa agaaatatta gtacctttta aaaagaaatt tgaggtacaa catatgccta
     2881 tttataatga tgacaaacag acaatgagtg cgattcaatt tttagatgcc tttcaaattt
     2941 ctcccaaaga tttacgcatg tctgaccaaa tgtcaataca atacctgaat aatgatttct
     3001 ggagtaattg cgaaaaatat ttaaaagttg ctatagatca attttcaaat tatagtatct
     3061 catctcaagt ttctaactat catttcacag tattacttgg agatcgccaa aaaccactta
     3121 tgtatctaaa taaaaatcgc ggtggtgatg gtggcatacc aggttatatt atgatttatt
     3181 tagtgccgag tacaagtaca attaattcta tgaaaagctt aattgcacat gaagtaaatc
     3241 ataatatgcg ctatcaatat attgattggg atggcggaag tttgattgaa ttgattattg
     3301 cagaaggatt agctgaaaac tatgtagaat cattgtatgg taaagcgcat attggaccgt
     3361 gggtaacaaa tactaattga agtcgtgata acgtaaaaat taaaaatact atttataatc
     3421 atttacattt aaagcatata tttgaatcga tgccttatct ctatggtgat gatattaata
     3481 aacttcaagg taggcctatc gttggcttat ctcatgctgc cgggtatgca tgtggctatc
     3541 acttggtaaa atacttttta caaaaaacaa acatacctat tgaagttgct acaacacttc
     3601 cagcacaaaa aataataaat gaggtgactg aattttggca tacacataca ctttaaaaga
     3661 tattattgaa attacaggtg taactaaaag aactttacat tattacgatg aaataggatt
     3721 attagttcca gataaaaatg ataaaaatta tcgcgtttat aaacagcaag acttagaaaa
     3781 attacaaaag attttaatac tcaagtcttt tgattttgat atcgctaaaa taaaacaata
     3841 catttcgtat gataatgaac aattgcgaaa gttattatca gagcaaataa gcaagttaga
     3901 taaaaagatt tctgacttac aattaattag acgctctgta tgtgaattta ttaaaggact
     3961 ctctctaata gataccagca ttttaaacaa gacactacag tcacaatatg ataaagaagc
     4021 atctataaaa tatggtcata cgaaagcata tcaatcattt attagacgta aagacagctt
     4081 acaatcgcag gatatcagac ataaattgac aactatcttc aataaattta atcatatgtc
     4141 tttgagtcat tatccaatcc aagattgtag tgatctcgta tttgagtgga aggcatttat
     4201 gaacactatc gctgattttg atgatgaaac attatgctgt attgctaaaa catatgaaga
     4261 tgatacgcgt ttcaaagatt actttaattc atatgataat caaaatttag catcatacat
     4321 ttcagaagct gttaattatt ttttgagcaa tgtgaataag agcgacaatt tttaatcctc
     4381 atacagatgc tacaacactg aatacaccaa ctaaaataat tgttcaaagt agaaaactaa
     4441 tcatttattc tctaaatcaa taactatatt aaaagttata cctttgcaaa gcgaattagt
     4501 ataggttacc gaaaggagaa aggattaggt tccattcgat ttataaaaaa atatatttcg
     4561 aaacatacaa aagcgccaga agataatcat ttcctctcgc gcttttatac tgaattatat
     4621 taatttcaaa ctctaaaact ctaataattt ctttttcaat gcatattcaa ctagctcagg
     4681 tttactcttt aagccaagct ttgtcataat atgcgtctta tgtgcttcta ctgttttcac
     4741 agatacaaat aatttctctg caatttcttt attcccgtaa cctttggcaa taagtggcaa
     4801 tatttctagt tctcgttttg ataagatttt aaaaggatct gttgtgtttg cagtgtcctg
     4861 atttgaatta gatacaaatt cattcactaa agatgttgta agtttcatat ctacatatgt
     4921 ttcaccttta tatacagttc gaatagctaa caataattgt tcatcaggtg catttttcaa
     4981 tatgtaacct ttcgcaccat tacgcaacac atggaacaaa tactcctcat catcaaacat
     5041 tgttaatatt agtattttag tttcaggaaa actgtcagca attttactcg tagcgataag
     5101 acctgactca cctggtggca tacttaaatc cattagtaac acatcaggtt tatattccat
     5161 tactttttgg taagcttcga cgccatctgc agccgttgca acaacttcca tatcattttg
     5221 ataatttaaa atcatagaga accccgtacg gacaacagcg tgatcatcgg caatgactat
     5281 tttcaatttt attcccccaa tgtatgtttc aaattggaat gttcaatgta acattggtac
     5341 cctcaccaat tttcgtttca atattgacgc taccgctgac taactcagct cgctcattca
     5401 ttccatataa accgagtcca gaacctttag gcttagaact tggatcaaaa ccatttcccg
     5461 catctatcac ttctgctacc aaatggcgcc cagtttgacg gatacctaca tttatttcat
     5521 ttacatcagc gtatttcaac gcatttaaaa tagcttcttg cactactcga taaacaaccg
     5581 tttcaatatc actatcaaag cgagtatttt taatatttga tgtatatatg atttttattc
     5641 cataattttc ttcaaactgt ttaaaatatg atttaaaagc tgcttcaagg cctagatcat
     5701 ccaaagaagc gggtcttaat tcaaccgaca tattacgtat atcatcaatt aatttagcga
     5761 caatatattc aatattttct gcgtcttcca aaagcttagt tgtatcttct tgatatttta
     5821 ataatctcaa ttgaacatct acattgagca tttcttgaat cacactatca tgtaactctc
     5881 tagaaattcg ctttctttca ttttcttggg ctgagattgt tttacgcatc atacgttgtt
     5941 gatgcaattt ctcttgctgt tcaatttgtg atgaaacatt ttgaagcgta aatgcatgaa
     6001 ttcccctgtc ttgatcaatc aactgatatg ttgctgtaaa tggcatcact ttttgatctt
     6061 tcgtcttcat aaatacttgg aaattcgtag cttgtacttg catcgattct aagaaacagt
     6121 ctttacatga ttgtacatca taggcatttg tatacccttc acatctgtga caaatcgcat
     6181 tcgccacagc actgtaatta tcttcttcag ataatatatc tttagcagca tcattcattg
     6241 caataatttt accgttatca tcagcaaaaa ctatcttttc gattgaatgc tcataatatt
     6301 ttttcaataa agtatctaac tgtatactgt cctcattaat catgacttac accctaattc
     6361 atctcattat ttatcatcat tgaaaatacc aaacttacgt tgaatatcat cattatcaaa
     6421 tatttttggt aaaggacgac catctctttg accaaataat agtacgccat acacttgatt
     6481 cttataccaa agcggcactg ctaaaactgc tgttaatgat tcgctcaata aaattggata
     6541 gtcaatattt tcttcaggcc ctaaagctaa accaacattg gctattacca tacgctttcc
     6601 tgttttcata acagttccag ctaatccacg accttttctt aaaataatca atttaaatcg
     6661 attattttta ttacctgaaa catagtgcca ttttattgga gatgatggtt tgttagattc
     6721 atagaaagcg attgccgcaa aatcataacc ctcttctttg cgtattttat ctaatgtctc
     6781 ttgaaatcta cgatcttcaa ttattgcttc tggtgtcaaa tcctttcacc tcttatgctt
     6841 acactttatt cttacggtaa ataatatatc tgcgatttat atatgtcaaa ggtacactcc
     6901 aaacatgcac caaacgtgta aatggccaac aagccataat agtgaaacct aacaatatat
     6961 gcattttaaa tgcaatcggc acaccactca tcaatgacgc atctggtttt aacataaata
     7021 attgtctaaa ccaaattgat aatgaagttc tgtagttaaa gtctggatgt tgtatatttg
     7081 ttactaatgt tgcgtaacat cccataaata cgataagtaa taataagaaa tttacaaata
     7141 tatccgacgc tgaacttaat cttcgaatac ttttcgtagt aacacgtctc gctgttaata
     7201 aaaacatccc tatcaaagtt attataccaa agatgctacc aatataaaca gcgcctatat
     7261 gatataaatg ctcagacaca cccactgcat ccatccatgg tttcggtatt aacaatccaa
     7321 ctacgtgtcc aaaaaacact ggaataatac ctaagtgaaa taataaactt ccccacatca
     7381 acctttttct ttctattaat tcactagatt tagctgtcca agaaaattta tcataacgat
     7441 aacgtgcaat atgacctgcg acaaagacaa ctaaacataa atacggaaat ataacccata
     7501 aaaactgatt aagcatgatg tttcactcct tttggtgatg tcaaacataa tttcaatgtt
     7561 tttctaagtg cttgaatcac ataggcatat ggattgttat cttcaccaag tgcattcgcc
     7621 atcacatatg ttccatcctc aataatcata atgattaatt gaatattctc ttcagctctt
     7681 ggatcatttc gccattctgc cacttgcaaa aattgaagca tcaacggtag ataatcagaa
     7741 agttcattat ctaccatttc tagtccaaac atttcatata atacctttaa tttagctaac
     7801 atttgcccac gttctttttg cgtatcaaat ttgttatacg tcatatataa tggtgctttt
     7861 ttcgtaaaat caaatgtatc tgtataaatc gctttgattt ctgataatga aaatgtcatc
     7921 attacatttc taaatgctac taaatcttca tatgctggat gatctttcga aattgtttct
     7981 tcaaaagttt ttggatgaaa agttaatttt tctggaaaac ataactgttg tgccatatat
     8041 ccaaaacttt cttgatattt tttaaaatta tcgaaattaa tcacggaaaa tccctccata
     8101 gaaattctca ttataaattt cttgaccagt tttccctgaa cctactgcaa cgccacagcc
     8161 ttcacagtta tctccaaaat gctcgccgcc gtaattgtat cctgtactac cttgtgcgtg
     8221 atacgtatct aaataggttt ctttgtgtga tgttggaata acaaatcgat cttcatattt
     8281 ggctagtcct aataaacgat acatgtcttt agtttggcgc tcggttatac ctaatcgctc
     8341 taatcgagac gtgtcaaatg gctgttgagt aacttgagat ctcatataac ttctcatcat
     8401 tgccatacgt tgtagggctc cttttactgg ctctgtatct cctgcagtga aaatattagc
     8461 taagtattca ataggtaaac gcatttcttc aatggctggg aaaatcgcat ctggattttg
     8521 agttgtattt ttaccttcaa aatagctcat aattgggcta agtggtgggc aataccaaac
     8581 catcggcatc gttctaaatt caggatgtaa cggaaatgca agtttatatt caattgctaa
     8641 cttataaatt ggagagtttt gtgcagcttc aatccaatcg taaccaatac catctttttc
     8701 agcttgagca atgacttctt cgtcaaatgg gtttaagaat atatctaatt gtttttcata
     8761 taaatctttc tcgtctactg ctgaagctgc ttcatgaact cgatctgcat catataataa
     8821 aacacctaag taacgcatac gtcctgtaca agtttcagag cataccgtag gcatacccgc
     8881 ctcgattctc gggaaacaga aagtacactt ttcagctttg ttcgttttcc aattgaagta
     8941 aactttctta tatggacaac ctgtcataca gtaacgccat ccacgacatg cgtcttggtc
     9001 aactaataca atgccatctt catcacgttt atacatagca cctgaaggac acgatgcaac
     9061 gcaacttgga ttcaagcaat gttcacataa acgtggtaaa tacatcataa aagtttcgtc
     9121 aaattggaat ttaatatctt cttctatttt ttggatgtta ggatcttttg gacctgtaac
     9181 atgaccacct gctaagtcat cttcccagtt aggtccccat tcaatttcaa tgttatcccc
     9241 cgtaatttct gaatacgctc tagcaactgg cgaatgcttc cctgatttcg cagttgttaa
     9301 atgttcataa ttatagttcc atggctcata ataatcttta attaatggca tatctgggtt
     9361 ataaaaaatt ttacctaaag caatttttga aattctactt ccagatttta attcaagttt
     9421 ccctttacga tttagtaccc aaccaccttt gtagtgttct tggtcttccc aacgtttcgg
     9481 ataccctaca cctggcttcg tttctacgtt gttgaaccac atgtactcag cacctggacg
     9541 atttgtccaa gtgtttttac atgtcacact acacgtatgg catcctatgc atttatctaa
     9601 atttaatacc atcgcaactt gcgctttaat cttcaagcca attaacctcc ttcatctttc
     9661 taactgctac atataaatcc ctttggttcc caattggtcc ataataatta aagtgataac
     9721 taatttgtgc gtatcctccg actagttgtg ttggtttcaa atggattcta gtcggcgcgt
     9781 tgtgtgaacc accacgtgta tctgtaattt ctgacccagg cgtttgaata tgtttatctt
     9841 gtgcatgata cataaacatt gtacctttag gcatacgatg cgaaataact gctcttgccg
     9901 ttacaacacc attacggtta tacacttcta gccaatcatt atcttggata tcgtgttttt
     9961 cagcatcttc atttgatatc caaaccgttg gaccacctct aaatagtgtc aacatatgct
    10021 tattatcttg atacattgag tgtatattcc attttccatg aggcgttaaa taacgcagta
    10081 ccaaagcatc tgtaccacct ttaattttct tatctctatt cccaaatacc attggcggca
    10141 atgtcggttt atatactggt aagctctccc caaattgttg gaaaacttcg tgatccacat
    10201 aataactttg acgtcctgtt aatgttctaa aaggtactag acgttctata ttcgttgtaa
    10261 atggtgaata tcgtcgacct tgtttatttg aacctgggaa tactgctgtc ggtattactt
    10321 ctcgtggttg tgaagttata tttaaaaacg aaattttctc agcagcgcgt tcgctagaaa
    10381 tatcttttaa cggcattcca gtttgttctt cgagatcttc atatgatttt tgtgataatt
    10441 taccattcgt agcagatgaa atacttagta ttgcatcagc tacattacgt gctgtatcaa
    10501 tacgtggacg attcgctctc acagaatcat catttgtatc actccacgta cctaacatac
    10561 tttttaattc ttcatattgt tcactgacac cgaaacttac accatgtgct ccaactttcc
    10621 ctttttcaag tacaggacca agcgtgacat atttgtcgta aattttagtg tagtcgcgtt
    10681 ctacaattgc aaagttaggc attgtacgtc caggtaccgc ttcaatttca cccttcgacc
    10741 aatctttcac tacgccgtat ggtgttgaaa tttcttgctt tgtatcatga ctaagtggag
    10801 ttgtcacaac atctttaaac gttccaggta aatagtcttt tgccatttct gaaaatgctt
    10861 ttgccaacgt tttataaata tcccagtctg aacgcgattc ccataacgga tcaatggcag
    10921 gattgaaagg atgtacatat ggatgcatat ccgttgatga taaatcatgt ttttcatacc
    10981 aagtcgctgc cggcaaaaca atgtcagaat ataacggtgt tgccgtcatt ctgaagtcta
    11041 aagagaccac taaatctaac ttacctgttg tttcttcacg ccacgtaatt tcttctggct
    11101 tttcatcttc atttggtgta gctaataacc ctgattttgt gccaagtaaa tgcttcataa
    11161 agtattcttg accttttgca gaacttgaaa ttaagtttga acgccatata aataatgatt
    11221 ttggatgatt ctttttcaaa tcaggatctt ctattgcaaa ttgtgtttgt tttgatttca
    11281 cttcatcaat tgcacgttgc aaaatcgctt catttgaatc tataccttca tctttagctt
    11341 cttctgcaaa caacaaacta tttttattaa attgtggata tgatggtaac caaccaagtc
    11401 tagctgctaa aacattataa tcagctggat gttgatgctt taactcctct gttttagcta
    11461 atggagattt taaacgatct acatttgact cttcatattt ccattggtct gttgcaaaat
    11521 agaaccaact tgttccgttt tgcaaacgtg gtggtccttg ccagtctttc gcaaatgcga
    11581 cagtactcca tccttcaatc ggacgacatt tttcttgtcc cacatagtga gcccaaccgc
    11641 caccgttcac accttgacag ccacataaca taactaagtt taagattgaa cgataaatcg
    11701 tatctgagtt aaaccaatgg ttaatacccg cacccatgat aatcattgaa cgcccttcag
    11761 tatcgatagc gttttgcgca aattctttcg ctacttgaat gacaacactt tgttttacgc
    11821 ctgaaatggc ttcttgccaa gcaggtgtat attttgattc tgcatcgtcg tatccttttg
    11881 attctaattt atgatcaaaa cgacgcacgc catattgact tgccattaag tcaaaaattg
    11941 tagcaatacg gactttgtca ccatttgcta aagtgacttg tcgagttgga attggacgat
    12001 tgaatatccc atctccatca ctatcaaagt atgggaattg aattgtttct aattcgtatc
    12061 caccttctgt cattgataat gtagggttaa ttttagaacc atcttctgtt tctagtttta
    12121 agttccactt cttaccttct tcccaacgtt gacccattgt gccattaggt actactaaac
    12181 tatcgctgat tgcatcatga ataactggct tccattcgcc ttgctctgtt gtttgaccta
    12241 agtcactcgc tcttaaaaat cgacccgctt tatatccatt ttcatcttca tccagcatga
    12301 taagaaacgg catatctgta tattgtttag cgtaatttat aaagcgttca ttaggttgat
    12361 taacataatg ttcttgtaaa ataacatgcg tcattgcttg tgcaattgca gcatctgaac
    12421 caggattcgg tgctagccag ttatctgcaa atttcacatt ttctgcgtaa tctggtgcta
    12481 ctgaaatgac ttttgtacct ttatagcgga cttcagtcat aaaatgtgca tccggagtac
    12541 gtgttaaagg tacattagag ccccacataa taatgtatga tgcgttatac cagtcacttg
    12601 attcaggcac atctgtttgc tctccccaaa tttgtggaga ggcaggtggt aaatctgcat
    12661 accagtcata aaaactaagc atttcaccac caagcaaatt gatgaatcga gcacctgctg
    12721 cataactaat cattgacatc gctggaatag gtgtaaatcc tgcgattcga tctggaccat
    12781 atttttttat tgtatacagt aattgtgctg cgattatctc tgtaacgtct ttccaatttg
    12841 aacgcacgtg ccctcccata cctcgggctt gcttatattg tttggctttg tcttcatttt
    12901 caacaataga cgcccatgca gcaacgcgat taccattgtt ttcttctaat gcttcagtcc
    12961 ataaatccca gagttttcca cgaatatatg gatatttgat tcgaagcgga ctgtattcat
    13021 accaagagaa tgacgcacct cgtggacatc ctctcggttc atattcaggc atatccggac
    13081 cacaacttgg atagtcagtt tgttgatttt cccaggtaat cacaccattt ttcacaaata
    13141 ctttccaaga acatgagcct gtacagttaa caccatgtgt tgttcttact tctttatcgt
    13201 ggctccaacg ttctctgtac attttttccc attctctact tttactttct aggatcgacc
    13261 aattcccatt aaatttttct gttggcttaa agaaattcaa tccaaatttt cccatattta
    13321 tatcctccta cgtataaaaa tacgatgtgt agatgtcgtg tttttaaata ctttaaaatg
    13381 cccaagacta ttgctttaat tagattgtac attttttcac aaacataaaa tattagggaa
    13441 tcacctaatt acttaaggaa tttccctatc aataacggga tttcattgaa ataatacaca
    13501 atcatgtatg gtcatgctta ttgccaatct aaatcgttca aatttggcac aacgacaaat
    13561 aaggcttcaa cacgaatata ttctctcggt tgaaacctta cttattcatt tattttttat
    13621 aaattagtga cataacactg tattagcatc tgcacgatcg gttgaaatat atgttacatt
    13681 ttcttgctgc ttaataaatg catcatagta atcatattgc gacgaatgat atgtgccatt
    13741 cgatgtatca tttgggttta gcaaacagcc ataaccttcg tcatataaat gttcacagag
    13801 cataagggcg tcatgtttag aaccacttac tacataaaat tgcttcatag gatcatatga
    13861 tttaggagtg ttttcagtat aatcaacaac ttcccctata atacatatac ctggtttcgc
    13921 ctcaattgaa tagtgttgca attttgaaat aatattactt aaacgcccct taacaacaaa
    13981 ctcgttaaaa cacgatgctt gaaagacaat cgctatcggg taatcaatat ctgtgtattg
    14041 ttgtatctgt gtgataattt tccctaaacg ttttaccccc atataaattg ctaacgtgcc
    14101 accattcact aaggaattga catccacttc attttcttct gaatctttaa agtgacctgt
    14161 agaaaatgtc acacttttag caactgtacg cattgtcaaa cctgtctgca tagtagcaac
    14221 tgctgcgctc gctgatgtca cccctggtac aatttcaaac gcaatatgat gttcatttag
    14281 tgtgtcgact tcttcttgca cacgaccaaa tatcgctgga tcgccacctt taagtctaac
    14341 aaccttgtta tatcgacgcg ctgcttccac gatacagtca tttatttttt cttgctgaat
    14401 atgttttgca tacggctttt taccaacatc gataatttca gtagtcaaat tcgcatattg
    14461 taaaattaac ggattcacta atcgatcata tagaatgaca tccgcttcac gtattaaacg
    14521 ctcagccttt ttcgtcaaat aattcggatt acctggaccc gcacctatca agtaaacctt
    14581 gccatattcc tctacagaca tatatatacg ttcccgtctg taacttctac ctcataaaca
    14641 tctacacaac cttcatcagg ttcttgaaca atacctgtat ttaaatcaat tttttgatcg
    14701 tggagcgggc aaaatacata ttccccactc actgtccctt cagacaatgg tccttgtttg
    14761 tgtggacaga tattgtgaat cgcatgaatt ttgccacttt ctgttaaaaa caaccctacc
    14821 tctttgcctt tgacaataac cttttttcca attaggggtg ttaattcatc tatagttgtc
    14881 actttaattt tttcttttgt ttccatgtat tacaccttct ccacttcaaa aattctacgt
    14941 gcttgcgcat tgctagttat tgcttcccaa ggttcagctt cgactgcttt tttagcatcc
    15001 ataatgcgtt caaatagttc attttgtctt tctgggtcaa gtaagacttc ttttacattt
    15061 tcaaatccaa gtcttcttaa ccatggcgct gttctttcag catatatacc tgtttcgcga
    15121 taatattgca tcaaagcacc acataatttg attacttcat cttctgtttc tacagttgtt
    15181 aaaaattcag ctttttcaac ttctgtacca ccattaccac cgatatagat ttggaatcca
    15241 ttttcaactg agataatacc aaaatcttta acacctgatt caacacaact tcttgggcag
    15301 cctgatacac ccattttgaa tttatgaggt gtatcgatgt attcaaatgt tttttctaaa
    15361 cgaatgccaa gtcgtgtcgt gtattgcgta ccaaatcgac aaaactcttt accaacacag
    15421 cttttaactg agcgtgtttt cttaccataa gctgatgctg aacgcatacc taggtcttcc
    15481 catatatttg gtaattcttc ttttttaact ccatacaaac caacacgttg tgaacctgtc
    15541 actttaacta gtggcacatg atatttctta gccacttctc ctagacgaat cagttggtct
    15601 gcatctgtaa cacccccacg catttgaggt ataacagaaa atgtaccatc attttgaata
    15661 ttcgcatggt aacgttcgtt agcaaatctt gattctcttt catcttcatg atcatgtgga
    15721 taaaccatgt ttaaataata gttgattgct ggtcgacatt ttggacatcc acctttattt
    15781 ttaaagttta aaacatgtcg aacttcttta gatgttttta aacctttcgc tcttatttgc
    15841 gttactattt gatcgcgtgt caaatcagta caaccacata taccagcagg ttttgcggca
    15901 acaaagtcat ctcctaaggt gtgctgcaat atttgagcaa tttgcggttt acatttacca
    15961 catgaattcc ccgcttttgt tttagccgtt acttcttcaa ctgttgtaaa gccattttcc
    16021 gtaatcgcat ttactatagt acctttatca acaccattac aaccacaaat tgtttcatca
    16081 tcagccatat cagcaattga tagcgatgcc tcttctccac ctttagtaag caatgataca
    16141 agtgtgtaat cttcagtgga ttcacctttt ttcatcatgt tataaaagcg tgaaccatca
    16201 tcgatatcac catatagtac tgcaccaact acattaccgt cttttaaaaa gattttttta
    16261 tagttattat caacactatt aaatatttca atacctttaa tttctgcatt ttctacaatt
    16321 tgaccagcac tatacaagtc acacccagaa acttttaatg acgtaaatgt tgttgatccc
    16381 ttgtatccgt tcgtttcttt atttgttaaa tgatcagcta atactttacc ttgttcatat
    16441 agtggtgcaa cgagtccata aactttgccg ttatgttctg cacattcacc aactgcatat
    16501 acattgctat cacttgtttg catcacatca ttgacaacaa taccacgatt aacatctaga
    16561 cctgattctt tggctacttc tgtgtatggt cgtataccta ctgccataac aactaagtct
    16621 gccggaatct cgcgtccatc agccaattta acaccctcaa catcatcttc tcctaagatt
    16681 tcagttgtgt tggcttgcat ttcaaacttc ataccttgct tttctagatc tgctttaagc
    16741 atatttccag ctttacggtc tagttgcatt tccatcaacc attcagctaa atgtaacacc
    16801 gttacttcca taccttgatc taataaacca cgtgcacact ctaaacctag taatcctcca
    16861 ccaattacaa ttgctttctt tttagtctta gcaatgttca tcatttgttc agtatcatca
    16921 agcgttctcc aaccaattac actcggtaat gtcgaacctg gaactggaat gacgaatgct
    16981 ttagaacctg tcgcaaaaat caatttatcg tatgatactt caataccatt tgcagtagta
    17041 actgattgat ttgctctatc tacttcaatt acaggatcat ttgtaattaa ctcgatacca
    17101 tgttcctcat accactcata tggattcata attgtttctt caactgtcat tttattttgt
    17161 aaaatatttg aaagcatgat gcggttatag tttggataag gttctttacc tattaccgta
    17221 atatcatata aatcgttggc gcgctctaat atttcttcga ttgttcgaat gcccgccata
    17281 ccgttaccaa tcattactag tttttgcttt gccataaaat atgccccttt actccataat
    17341 atttatttca aaaaaaggta ttaatttttc gttagtgctt ttatattttc attggaatca
    17401 ttaagctttc taatctatcg ttaatgattt gctttaaaat tgggtcgaag ttaattgaag
    17461 gtgtgaagtg tatatctgta ttaataacca tgtcattcat ttgctgcttc actttgttaa
    17521 caagtcttcc gtcatataaa aataatggta cgacaatcaa tttttgatac cgtttcgaga
    17581 tgctttctaa atcatgtgta aaactaatct ctccatatag cgttctcgca tatgtcggct
    17641 tgctaatttg caaattttga gcgcatattt gtaactcttc gtgtgcctta gtaaactttc
    17701 cattaatatt gccgtgtgca acaaccataa ctccaacttg ttgttcgtca cctgctaatg
    17761 cgtcacaaat acgttgttca attaatcgtc tcattaaagg atgtgtgcca agtggctcgc
    17821 ttacttctac ctttatgtct ggataccgtc gtttcatttc atgaacgata ttcggtatat
    17881 ccttgagata atgcattgca ctaaagatta gcaatggtac aattttaaaa tggtcaaccc
    17941 cactttgaat caacgtcgtc attaccgtct ctaaatcctg atgctcactt tctaaaaacg
    18001 caatatcata gtgatgtata tcatctttta ctaattcaga aataaatgct tctaacgctt
    18061 gattctgtcg tccgtgcctc atgccatgtg caacaatgat attcccattc acatttacca
    18121 accctttcac acgtattgta taccaaatca ttttgttttt gtgaaaagaa tcacattata
    18181 atgtaaaatc agggaattcc ctgatgcctg tagtcatgca tattccttat acattttccc
    18241 tttttgttaa atcaaaaaaa gcgaccgata tatgaatccc tactcaacat ttatttgagc
    18301 aagcattaat atatcggtcg cttgtagtgt atattattat cttaaaatgg tggttggcct
    18361 aatattgttt cgtcaaagcg ctcgggtatc aatactttgc gcatgatcac acctaaatcg
    18421 ccatcatcat tttcatgttc gctgtatatt tcataacctc ttttttcata aattttaagt
    18481 aaccacggat gcaatcttgc agatgtacct aaagtaactg ccgctgactt taacgtatct
    18541 cgcaaaaatg cttcttcaac ataagtaagt aattggctac catagccttt cccttcatac
    18601 tcaggatttg tcgcaaacca ccagacaaaa ggataacccg aaatactttt cacacttccc
    18661 caaggatatc taaccgtaat cgtagatata atttcatcat caattgtcat gacaaatgta
    18721 gtatttttat ctatattttc tttaacagca tctaaattag cattaaccga aggccaatca
    18781 atacctagtt ctcttagagg cgtaaatgct tcatgcatga gttgttgcaa tttttctgca
    18841 tcttgttcac ttgcgagtcg aatcatcgtt tttgtcatat taatccccac tcttttttaa
    18901 atgatttaac catattttat ttttaaaata aatatccatc aaagtgtatc aataaattta
    18961 tcacatgtca gaaagtatgc ttcatctgaa tacaccaata ctctcatgaa acttattaaa
    19021 aattactctc tcaacgtaaa aaaccattca aattcatgaa tggtttggaa gaatgattca
    19081 ttgttacgct atttaatcac tacatcttaa ttattgttgc tctaaacgat tacgcttacc
    19141 atttaagaaa gcataaacga gacctacaaa aataccgcca ccgacaaagt tacctaagaa
    19201 agcaaaaacg atatttttta aaacgtgtaa ccatgaaact gcatcaaggt taaagaatac
    19261 catacctgca tatagacctg cattgaacac aacgtgctca tatcccatgt atacaaagac
    19321 cacgacacca caagctatga agaatgcctt tgttaagccg cctttgaatt gcatagagat
    19381 gaaaatacca atattaataa agaagttaca gaaaatacct tttgtaaaaa tattcaacca
    19441 tgttgaatca acagtctttt tctgaactaa agctgttaaa gcttgtgtca tttcaggtgt
    19501 catgacatgc gcaaatttca ttaagaaaaa taacacaaac ccacctaaaa tatttcctaa
    19561 gaaacaatat aataaaatcc aagtcatctt cttaacagaa acgactttat aataccagcc
    19621 tactgtaaag tacatgaagt tacttgttaa taattcagag ttagttaata caactaaaat
    19681 caaacctaaa ctaaacgcaa tggctcccat taaattgata agtccatcta cgtgagtact
    19741 cgcaaactgc gtctttattc cgaacataaa tacagtaacg attgagagta aaaatccggc
    19801 catcattgct ttaagcatat atcggccagg tgtttgctgt accattactt gcttcatttg
    19861 tactgaagat gatattgaat caacaattgc gcctgttgaa tatgtatctt caactgtttt
    19921 cttattttct atcacagtac ttcccccatt tactttgatc aaataaaatt tactttctaa
    19981 ttatattagt aaatcaatgt aattgataga ttaaatttca gaaaaatctt tttattctct
    20041 cgaaaatgta gttttctttc ataaaaacag cgagataaat cgcgtgattg gctaatatct
    20101 ttatgattta agatattacg acgctcattt tatctcgctt tatgtaaagt attacttatt
    20161 cttttttaaa tttgaaaagt aatgtaaacc atatgcaaat acactgtaac caattgcagt
    20221 acgtaaaata aaccttataa ctttattagg tacacatttt gacgttactg aaatagaaga
    20281 tagtagtgtt gtgaaaatat ataatattaa aactttgata taacgttcta ccatcgaatc
    20341 atccctcatt tttataatat tacaagatta ttataaaccg aatgaaacaa aaaagttttt
    20401 acatattttc aacaacttta tttcaataaa gcatcaagtt gatctaaagt ttgattcata
    20461 ccttgttcaa cacccatgtt tatgacttgt tgagcggctt cttttgttgg aaataccgat
    20521 gttgatgtca ctgtcgtttt cgttgtattg ctcttggaaa atgacaaagt aattttcatg
    20581 cttggcattt ttgtatcttt tgcaccttga ggtgtcgcaa atgagtcaat atattcaatt
    20641 aaatatggac gcttaaccgt tttatattct gctaatgtat agctggtcat tgtaggtgtt
    20701 ttaatcgcat aaaatgcatc accgcctgaa acagcattaa agcgaaacac tttggtacta
    20761 gcgtcttttg gatgaaacca tttttcaaat aatgcttttg tcgtataggc atcaaacacc
    20821 ttttcaatcg gtgcttctat cgttcttgaa aaaataattt tattatcttc aactttaatc
    20881 gtcatcttcc cactccctat cttttatatt tactcatctt aacatgcatt gagtaacata
    20941 tgttaccaaa tcatttgtta taaactatag taatattaat cattccctct gttgaaattg
    21001 ataactacga aataaaaaac acactctatt cagctattaa cagttgagta gagtgtgcaa
    21061 acctgttgaa ttaatgcgct aacatttctt ctttgatttg gtctttattt aattttgaag
    21121 gataatatgt tggccagtta tccatttctt ttaataacgc gtcattatca ttacccataa
    21181 agatatggaa gtgttctgcc tttttaggtg caatgttgtg atcgctaaat tggatgaatt
    21241 tcggtaagtc tttatcatta ccatcgacca atttaaacat aaatctcacg ccacggttac
    21301 ctttaggata tttcaatgtt ttcttaccat tgtattcata tttaccagtg tgtttcttac
    21361 catctttagt aaatgtaatt tcatttcctt ttatatcaat gttagtaata tcagttttat
    21421 atcctttgtc ataataagct tttaaatctt tagcagattt cttcggatca ttttcagctt
    21481 tatgttccat cacttcatca agcgtaccgt cttttaagta agggtaaaca gattgccatt
    21541 caccagcata atcgcttaat tcacggtctt taacttgctc atctttaaaa tacccatcag
    21601 aaattgcttt gtcatgttta ctttcagctt tgtcgtcttt cactttaaca ccactgtcta
    21661 aagctttacc gatattttca atattaga
//
LOCUS       DAKTIO010000027        21592 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.027, whole genome shotgun sequence.
ACCESSION   DAKTIO010000027 DAKTIO010000000
VERSION     DAKTIO010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21592
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.027"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(30..296)
                     /locus_tag="O0293_000774"
     CDS             complement(30..296)
                     /locus_tag="O0293_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCY0279670.1"
                     /translation="MSNYTVKIKNSAKSDLKKIKHSYLKKSFLEIVETLKNDPYKITQ
                     SFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLILSAWSHYD"
     gene            complement(296..553)
                     /locus_tag="O0293_000775"
     CDS             complement(296..553)
                     /locus_tag="O0293_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCY0279671.1"
                     /translation="MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLE
                     DWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDIDWDNL"
     gene            complement(659..853)
                     /locus_tag="O0293_000776"
     CDS             complement(659..853)
                     /locus_tag="O0293_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279672.1"
                     /translation="MMLFKNLNFVIFFEDMSFFKRIPHKILKIFKPENDKAVGNVVCS
                     TADFFLSIIGGMLEEKNKSL"
     gene            912..1367
                     /locus_tag="O0293_000777"
     CDS             912..1367
                     /locus_tag="O0293_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1307 domain-containing protein"
                     /protein_id="HCY0279673.1"
                     /translation="MKKLCSLIVVALVCIIALSACGKEQTKTYEGDVSGKHVLTSITY
                     KDDKVLKQSTINTIKYDDLGMDKDEAKKLFAKSESIFKDLKGVKYKVDYKDKKAIEHL
                     DIDYTEVDMKKLNKRLGVSTKENKDISFEKLEKQLKHRGLKEKDKMDDK"
     gene            complement(1535..3112)
                     /locus_tag="O0293_000778"
     CDS             complement(1535..3112)
                     /locus_tag="O0293_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HCY0279674.1"
                     /translation="MTFLTVMQFIVNIIVVGFMLTVIVIGLIWLIKDKRQSQHSVLRN
                     YPLLARIRYISEKMGPELRQYLFSGDNEGKPFSRNDYKNIVLAGKYNSRMTSFGTTKD
                     YQDGFYIQNTMFPMQRNEISVDNTTLLSTFIYKIANERLFSREEYRVPTKIDPYYLSD
                     DHAIKLGEHLKHPFILKRIVGQSGMSYGALGKNAITALSKGLAKAGTWMNTGEGGLSE
                     YHLKGNGDIIFQIGPGLFGVRDKEGNFSEGLFKEVAQLSNVRAFELKLAQGAKTRGGH
                     MEAEKVNEEIAKIRNVEPYKTINSPNRYEFIHNAEDLIRFVDQLQQLGQKPVGFKIVV
                     SKVSEIETLVRTMVELDKYPSFITIDGGEGGTGATFQELQDGVGLPLFTALPIVSGML
                     EKYGIRDKVKLAASGKLVTPDKIAIALGLGADFVNIARGMMISVGCIMSQQCHMNTCP
                     VGVATTDAKKEKALIVGEKQYRVTNYVTSLHEGLFNIAAAVGVSSPTEITADHIVYRK
                     VDGELQTIHDYKLKLIS"
     gene            3307..4056
                     /locus_tag="O0293_000779"
     CDS             3307..4056
                     /locus_tag="O0293_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279675.1"
                     /translation="MSNTNKHYIEEEYATEQSRFFKRDIGFIFLYIFLVNILPIIIVF
                     LTFGLVAVFTQQAPWGFSFPISMNISYLLAVIISLAIFRLMHEDAFIPMIRSQIVASA
                     KYKWHILISFTLSVVLFLLLNHFNIFALNVDSFTVFDIYPLIKGYGSISLITIFSLTI
                     ILNTLICQLIFRHILIQELSRLIPLKITIVLSIILETLFYYPYINTWWEFIPALILAG
                     SATYLYLKSYRNFMVSYFYQLAVILVLHIFM"
     gene            complement(4280..5473)
                     /locus_tag="O0293_000780"
     CDS             complement(4280..5473)
                     /locus_tag="O0293_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0279676.1"
                     /translation="MKGAMAWPFLRLYILTLMFFSANAILNVFIPLRGHDLGATNTVI
                     GIVMGAYMLTAMVFRPWAGQIIARVGPIKVLRIILIINAIALIIYGFTGLEGYFVARV
                     MQGVCTAFFSMSLQLGIIDALPEEHRSEGVSLYSLFSTIPNLIGPLVAVGIWNANNIS
                     LFAIVIIFIALTTTFFGYRVTFAEQEPDTSDKIEKMPFNAVTVFAQFFKNKELLNSGI
                     IMIVASIVFGAVSTFVPLYTVSLGFANAGIFLTIQAIAVVAARFYLRKYIPSDGMWHP
                     KYMVSVLSLLVIASFVVAFGPQVGAIIFYGSAILIGMTQAMVYPTLTSYLSFVLPKVG
                     RNMLLGLFIACADLGISLGGALMGPISDLVGFKWMYLICGMLVIVIMIMSFLKKPTPR
                     PASSL"
     gene            complement(5485..6234)
                     /locus_tag="O0293_000781"
     CDS             complement(5485..6234)
                     /locus_tag="O0293_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0279677.1"
                     /translation="MIKIKDVEKSYQSAHVFKRRRTPIVKGVSFECPIGATIAIIGES
                     GSGKSTLSRMILGIEKPDKGCVTLNDQPMHKKKVRRHQIGAVFQDYTSSLHPFQTVRE
                     ILFEVMCQCDGQPKEVMEVQAITLLEEVGLSKAYMDKYPNMLSGGEAQRVAIARAICI
                     NPKYILFDEAISSLDMSIQTQILDLLIHLRETRQLSYIFITHDIQAATYLCDQLIIFK
                     NGKIEEQIPTSALHKSDNAYTRELIEKQLSF"
     gene            complement(6227..7042)
                     /locus_tag="O0293_000782"
     CDS             complement(6227..7042)
                     /locus_tag="O0293_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0279678.1"
                     /translation="MTLLTVKHLTITDTWTDQPLVSDVNFTLTKGETLGVIGESGSGK
                     SITCKSIIGLNPERLGVTGEIIFDGTSMLSLSESQLKKYRGKDIAMVMQQGSRAFDPS
                     TTVGKQMFETMKVHTSMSTQEIEKTLIEYMDYLSLKDPKRILKSYPYMLSGGMLQRLM
                     IALALALKPKLIIADEPTTALDTITQYDVLEAFIDIKKHFDCAMIFISHDLTVINKIA
                     DRVVVMKNGQLIEQGTRESVLHHPEHVYTKYLLSTKKKINDHFKHVMRGDVHD"
     gene            complement(7039..7908)
                     /locus_tag="O0293_000783"
     CDS             complement(7039..7908)
                     /locus_tag="O0293_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0279679.1"
                     /translation="MIILKRLLQDKGAVIALGIIVLYVFLGLAAPLVTFYDPNHIDTA
                     NKFAGMSFQHLLGTDHLGRDILTRLIYAIRPSLLYVFVALFVSVLIGSILGFLSGYFQ
                     GFVDALIMRACDVMLAFPSYVVTLALIALFGMGAENIIMAFILTRWAWFCRVIRTSVM
                     QYTASDHVRFAKTIGMNDMKIIHKHIMPLTLADIAIISSSSMCSMILQISGFSFLGLG
                     VKAPTAEWGMMLNEARKVMFTHPEMMFAPGIAIVIIVMAFNFLSDALQIAIDPRISSK
                     DKLRSVKKGVVQS"
     gene            complement(7905..8840)
                     /locus_tag="O0293_000784"
     CDS             complement(7905..8840)
                     /locus_tag="O0293_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0279680.1"
                     /translation="MFKFILKRIALMFPLMIVVSFMTFLLTYITNENPAVTILHAQGT
                     PNVTPELIAETNEKYGFNDPLLIQYKNWLLEAMQFNFGTSYITGDPVAERIGPAFMNT
                     LKLTIISSVMVMITSIILGVVSALKRGKFTDRAIRSVAFFLTALPSYWIASILIIYVS
                     VKLNILPTSGLTGPESYILPVIVITIAYAGIYFRNVRRSMVEQLNEDYVLYLRASGVK
                     SITLMLHVLRNALQVAVSIFCMSIPMIMGGLVVIEYIFAWPGLGQLSLKAILEHDFPV
                     IQAYVLIVAVLFIVFNTLADIINALLNPRLREGAR"
     gene            complement(8853..10451)
                     /gene="cntA"
                     /locus_tag="O0293_000785"
     CDS             complement(8853..10451)
                     /gene="cntA"
                     /locus_tag="O0293_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine-dependent metal ABC transporter
                     substrate-binding protein CntA"
                     /protein_id="HCY0279681.1"
                     /translation="MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDI
                     GDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAKKWDVSEDGKTYTFHLRDDVKFH
                     DGTPFDADAVKKNIDAVQENKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAE
                     LAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSK
                     LNKVQAKVMPAGETAFLSMKKGETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQP
                     MNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQEKPATQLFAKNVTDI
                     NFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEY
                     LQAEFKKMGIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKAKNGY
                     ESATSGIENKDKIYNSIDDAFKIQNGKERSDAYKNILKQIDDEGIFIPISHGSMTVVA
                     PKDLEKVSFTQSQYELPFNEMQYK"
     gene            complement(10594..11895)
                     /gene="cntM"
                     /locus_tag="O0293_000786"
     CDS             complement(10594..11895)
                     /gene="cntM"
                     /locus_tag="O0293_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_096659686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine dehydrogenase CntM"
                     /protein_id="HCY0279682.1"
                     /translation="MSKLLMIGTGPVAIQLANICYLKSDYEIDMVGRASTSEKSKRLY
                     QAYKKAKQFEVKIQNEAHQHLEGKFEINRLYKDVKNVKGEYETVVMACTADAYYDTLQ
                     QLSLETLQSVKHVILISPTFGSQMIVEQFMSKFNKDIEVISFSTYLGDTRIVDKEAPN
                     HVLTTGVKKKLYMGSTHSNSTMCQRISALAEQLKIQLEVVESPLHAETRNSSLYVHPP
                     LFMNDFSLKAIFEGTDVPVYVYKLFPEGPITMTLIREMRLMWKEMMVILQAFRVPSVN
                     LLQFMVKENYPVRPETLDEGDIEHFEILPDILQEYLLYVRYTAILIDPFSQPDENGHY
                     FDFSAVPFKQVYKNEQDVVQIPRMPSEDYYRTAMIQHIGKMLGIKTPMIDQFLTRYEA
                     SCQAYKDMHQDQHLSSQFNTNLFEGDKALVTKFLEINRTLS"
     gene            complement(11888..12706)
                     /gene="cntL"
                     /locus_tag="O0293_000787"
     CDS             complement(11888..12706)
                     /gene="cntL"
                     /locus_tag="O0293_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-histidine (S)-2-aminobutanoyltransferase CntL"
                     /protein_id="HCY0279683.1"
                     /translation="MNNFNNEIKLILQQYLEKFEAHYERVLQDDQYIEALETLMDDYS
                     EFILNPIYEQQFNAWRDVEEKAQLIKSLQYITAQCVKQVEVIRARRLLDGQASTTGYF
                     DNIEHCIDEEFGQCSIASNDKLLLVGSGAYPMTLIQVAKETGASVIGIDIDPQAVDLG
                     RRIVNVLAPNEDITITDQKVSELKDIKDVTHIIFSSTIPLKYSILEELYDLTNENVVV
                     AMRFGDGIKAIFNYPSQETAEDKWQCVNKHMRPQQIFDIALYKKAAIKVGITDV"
     gene            complement(12717..13538)
                     /gene="cntK"
                     /locus_tag="O0293_000788"
     CDS             complement(12717..13538)
                     /gene="cntK"
                     /locus_tag="O0293_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine racemase CntK"
                     /protein_id="HCY0279684.1"
                     /translation="MNRQVIEFSKYNPSGNMTILVHSKHDASEYASIANQLMAATHVC
                     CEQVGFIESTQNDDGNDFHLVMSGNEFCGNATMSYIHHLQESHLLKDQQFKVKVSGCS
                     DLVQCAIHDCQYYEVQMPQAHRVVPTTINMGNHSWKAIEIIYETYVHYVIPVKQVTTE
                     IQHLVEAFVREQQWSHKYKTVGMMLFDEQRQFLQPLIYIPEIQSLIWENSCGSGTASI
                     GVFNNYQRNDACKDFTVHQPGGSILVTSKRCHQLGYQTSIKGQVTTVATGKAYIE"
     gene            complement(14405..14770)
                     /locus_tag="O0293_000789"
     CDS             complement(14405..14770)
                     /locus_tag="O0293_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279685.1"
                     /translation="MLKEKESFRLLYQAIREIADKIGDNQLETNSVSLLLLDFDFEHE
                     VFDELYLVILKYLNTVSIENISHSELLNLIENTIPEDREINTFVKNKIIIGFANNYFP
                     ELQVLANEIKSDMASSLKQ"
     gene            complement(15033..15851)
                     /locus_tag="O0293_000790"
                     /pseudo
     CDS             complement(15033..15851)
                     /locus_tag="O0293_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002503368.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            16084..17622
                     /locus_tag="O0293_000791"
     CDS             16084..17622
                     /locus_tag="O0293_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCY0279686.1"
                     /translation="MTSKHQQKGSIVNRFLNSVEKIGNKLPDPSVLFFLMCVGLAIMT
                     WVISLFNVSVKHPGTHQTIYIKNIISHDGFTMIMNDTIKNFSEFPALGLVLAVMIGIG
                     VAEKTGYFDKLMISVVNRAPRFLILPTIILIGILGSTAGDAATIILPPLAAMLFIKIG
                     YHPIAGLTMAYASAVGGFAANIVVGMQDALVYSFTEPATRIVSDSIKTNVAMNWYFIA
                     ASVVVLLPTILLVTTKLIIPRLGKYDDSLMHDDHEETSSHITDKEAHALKWANISFIV
                     TIILLIITAIPEHSFLRNAKTGSLLDDAPLINGVGLIILVVFLVPGLVYGILSKEIKN
                     TKDLGKMFGDAVGSMGTFIVIVFFAAQLLAYLKWSNLGIIAAVKGAKLLEHQNGIVLI
                     LGIIVLSAMVNMLIGSASAKWGILGPIFVPMLILIGFHPAFTQVIYRVGDSITNPITP
                     MMPYLPLLLTYAQKYDKRMKLGALLSSLMPYSIALSIVWTVFVIIWFLLGIPVGPGGP
                     IFVK"
     gene            complement(17734..18156)
                     /locus_tag="O0293_000792"
     CDS             complement(17734..18156)
                     /locus_tag="O0293_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCY0279687.1"
                     /translation="MPYNYKKQNGELMSVMSQGEKFIHQSPVNDELSALIKLLISKIN
                     GCHYCVDIHKKELKELGVTQMKIDEVLSFRHLDLFTDQEKVTLEFAEMLNSIKDFKKF
                     EIIDRLKSFYDEEQIIDLVFVVNQINGWNRLNIISDRL"
     gene            complement(18608..19027)
                     /locus_tag="O0293_000793"
     CDS             complement(18608..19027)
                     /locus_tag="O0293_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCY0279688.1"
                     /translation="MSKKHVFIIIGVILCICTVSTVMHFKMKYDEKEKQKAIYYKEQQ
                     ERITLYLKHNTKETNTIKSVHFTNLETSPMGSAVIEGYINENKEDDFTAYASPEHNYQ
                     FGGAMIKSEGVDKLLKPAHERKSPEKIKEELDKKEGH"
     gene            complement(19045..19464)
                     /locus_tag="O0293_000794"
     CDS             complement(19045..19464)
                     /locus_tag="O0293_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCY0279689.1"
                     /translation="MSKKKILILTSIMLIILISVAGIYLKVKYDEKEKQKAIYYKEQQ
                     ARITLYLKHNTKEPNTIKSVHFTNSETSPMGSAVIEGYINENKKDNFTAYATPEHNYQ
                     FGGAMIESEKLSELLKPANQLKSPDDIKKELNKKKSH"
     gene            complement(19731..20153)
                     /locus_tag="O0293_000795"
     CDS             complement(19731..20153)
                     /locus_tag="O0293_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCY0279690.1"
                     /translation="MNKKHVFIIIGVILCICIVASVIYLKVKYDEKEKQKAIYYKEQQ
                     ERITLYLKHNTKEPNTIKTVHFTSLKRGPMGDAVIEGYINENKEDDFVAYGSPEHNYQ
                     FGGSLIKSKNLSTLLKPVHQTKSPDEIKKELESKKNDR"
     gene            complement(20404..21099)
                     /locus_tag="O0293_000796"
     CDS             complement(20404..21099)
                     /locus_tag="O0293_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCY0279691.1"
                     /translation="MTVLTDKVAVVTGAGSGIGEAIATLLHEEGAKVVLAGRNKEKLQ
                     NVANQLSQDSVKVVPTDVTNKEEVDELIKIAQQTFGGLDIVINSAGQMLSSKITDYQV
                     DEWDSMIDVNIKGTLYTAQAALPTMLEQSSGHLINIASISGFEVTKSSTIYSATKAAV
                     HTITQGLEKELAKTGVKVTSISPGMVDTAITAAYNPTDRKKLEPQDIAEAVLYALTQP
                     KHVNVNEITVRPV"
     gene            21388..>21592
                     /locus_tag="O0293_000797"
     CDS             21388..>21592
                     /locus_tag="O0293_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCY0279692.1"
                     /translation="MTYHERVLALRAESKRTAFDFRFEDLFSKEEWLSMSLAERQKAE
                     KAFRHEVKNMDDVRMPFSSVHDAQ"
BASE COUNT         6778 a         4080 c         3161 g         7573 t
ORIGIN      
        1 attaaagtta ttcagatatt gaatattcat taatcataat gtgaccatgc cgatagtatt
       61 aatacttctt tatttcgatc atctacggta tagacgaccc tgtgctgatg gttaattctt
      121 cttgaatatc gctctaaata tttaggctct aatttttcaa aagattgtgt tattttatac
      181 ggatcatttt ttaaagtctc aacaatttct aaaaatgact tctttaaata agaatgtttt
      241 attttcttta aatctgattt cgctgaattt ttaatcttaa ccgtgtaatt gctcattaaa
      301 gattatccca atcaatatca tctatatttg ttgtaccact attatctttt tctctttctc
      361 ttactttgtc cattgtacca gtagattcaa gatatattgt ctcttgtata cttttccaat
      421 cttctaaacc tataatcaca gcattatttt cggcattatt gccactaata taaattggtt
      481 cgtgattatt attaacattt tttagtaatt gataaaaatc ttttctcgct tctgtagggc
      541 tagtgataat catataaacc ctcctttgtt ttctgttacg ctaattatag cgtacgcact
      601 tgagtacgtc aatattacca acgagttcat cgtattctta atacctatat gctatcgttc
      661 acaaactttt gttcttttct tccaacatac cgcctataat gctcaaaaag aaatcagcag
      721 tggaacacac tacgttccct accgctttat cattttcagg cttaaatatt tttaaaattt
      781 tgtgaggaat ccgcttaaaa aaactcatat cttcaaaaaa aatgacaaaa tttaaatttt
      841 tgaataacat cattttttag taaatgttat aatttcttta taatacaaat atcgaaagaa
      901 ggcgactgac tttgaaaaaa ttatgttcat taattgtagt agcattagtt tgtatcattg
      961 cattatcagc ttgtggtaaa gaacaaacca aaacatatga aggtgatgtt agtggtaaac
     1021 atgtactaac ttctatcaca tataaagatg ataaagtttt aaaacagtct acaatcaaca
     1081 caattaaata tgacgatcta ggtatggata aagacgaagc caaaaagtta ttcgccaaat
     1141 ctgaaagtat tttcaaagac cttaaaggcg taaaatacaa agtagactat aaagataaaa
     1201 aagcaattga acacttagac atagattaca cagaagttga catgaaaaaa ttaaataaac
     1261 gtcttggtgt ttcgactaaa gaaaataaag atattagttt tgaaaaactt gaaaagcaat
     1321 taaagcacag aggtttaaaa gaaaaagata aaatggacga caaatagttt ataacttaaa
     1381 aatgccctca gataagacta aggttacaaa ccttaattca tattctgagg gctttaatat
     1441 ttgaagttct tgtgtgacca gcatccacta ctaatataaa attatttgca gtaacgctaa
     1501 aatccgctgc tttcaatttc ccgaaataat taagttaact aatgagtttt aatttataat
     1561 catgtatcgt ttgtaactca ccatcgactt ttcgatatac aatatgatca gcagtaattt
     1621 ctgtaggact ggatacgcca acagctgctg caatattgaa taagccttca tgcaaacttg
     1681 ttacatagtt tgtgacacga tattgctttt ctccaacaat caatgctttt tctttcttcg
     1741 catctgtcgt tgcaacacct acaggacacg tattcatgtg acattgttga ctcattatac
     1801 aaccgacact aatcatcatc ccacgtgcga tatttacaaa atctgcacct aaacctagtg
     1861 caatcgcaat tttatctggt gtcactaact taccagatgc cgccaatttc actttatctc
     1921 gaataccata tttttctaac atgccagaca caataggtag agctgtaaat agcggtaagc
     1981 caacaccatc ttgtaattct tggaatgttg caccagtacc accttcacca ccatcaatcg
     2041 taataaagct tggatactta tctagttcca ccatcgtacg tacaagtgtt tcaatttctg
     2101 aaactttgct tactacaatt ttgaatccta ctggtttttg acctaattgc tgcaactgat
     2161 cgacgaaacg aatcaaatct tcagcattat gaataaattc gtaacggtta ggtgaattga
     2221 ttgttttata aggttcaaca tttcggattt tagcaatttc ttcgtttacc ttttcagctt
     2281 ccatatgacc accacgagtc ttagcacctt gtgccaactt cagctcaaat gcgcgtacgt
     2341 tagataactg tgcaacctct ttaaataaac cttcactaaa attaccttct ttatcacgaa
     2401 caccaaataa accgggacca atttggaaaa tgatatcccc attacctttt aaatgatatt
     2461 ctgataagcc accttcacct gtattcatcc aagtgcccgc tttagctaga cctttagata
     2521 aagctgtaat ggcatttttt cctaaagcgc cataactcat accagattgt cctacgatac
     2581 gttttaaaat aaatggatgt tttaaatgtt cacctaattt tattgcatgg tcatcactta
     2641 agtaatacgg atcaatcttt gtcggcacac gatattcttc acgactaaat aaacgctcat
     2701 tcgcgatttt ataaatgaat gttgataaca atgttgtatt atctactgaa atctcattac
     2761 gttgcatcgg aaacattgtg ttctgtatgt aaaagccgtc ttgataatct ttagtagtac
     2821 cgaagctggt catacgagag ttatattttc cagccaaaac gatattttta taatcattac
     2881 gtgaaaaagg tttcccttca ttatccccag aaaataaata ctgacgtaat tccggtccca
     2941 ttttttctga aatatatcta atacgtgcta gtaaaggata attccttaat acactatgtt
     3001 gtgattgtct tttatcttta attaaccaaa taagcccgat aacaataacc gtaagcatga
     3061 atcctacaac gataatgtta actataaatt gcatgactgt aagaaacgtc attacaatac
     3121 ctccccccaa aatttcaatt caatatttat gatacacctt acaaaacaaa acacaatgga
     3181 agcgcttcat tttataaaac aattttatga tatgtttttc attttaaatt ttaatgtata
     3241 aaacatacaa tacaaagtaa tatgtgctaa agtatctata taatacaact atttaagagg
     3301 tatactatgt caaatacaaa taaacattac atagaagaag aatacgctac cgaacaatcg
     3361 cgttttttca aacgtgatat tggatttatt ttcctttaca tatttttggt taacatcttg
     3421 ccgatcatta tcgtcttttt aacttttggg ttagtagctg tattcactca acaagctccg
     3481 tggggtttct cttttcctat ttcaatgaac attagctatt tactcgcagt tattatttca
     3541 ctagctattt ttcgtctgat gcatgaagat gcgtttattc caatgatacg ttcgcaaatc
     3601 gttgctagtg ctaaatataa atggcatatt cttatatcat ttacactaag tgtcgtactc
     3661 tttttacttt taaatcattt caatattttc gcactaaatg tagattcctt tactgtcttt
     3721 gacatttacc cgctcattaa agggtatggt tccatcagtt taataactat attttcgtta
     3781 accattatat taaatacttt gatttgtcaa cttattttcc gtcatatttt aattcaagaa
     3841 ttaagcagat taataccttt aaagattaca atagtattgt ccattatcct tgaaactctg
     3901 ttctactatc catacattaa tacatggtgg gaattcattc cagcattgat actagcaggt
     3961 agtgcgactt acttatattt aaaatcatac cgtaatttta tggtgagcta tttctatcaa
     4021 ttagcagtta tactcgtttt gcatattttc atgtaaaatt tttcagagta ttatccatta
     4081 tccatacaca tcaaaaagca cacttaaaag tcagtatcaa aactcacttt taagcgtgct
     4141 ttatttagat atactgtcgt tctaaaaacg agccatatag aatgtttcat gtcctagcat
     4201 gaagcacatg aatcgccatg ctcaatatac cttttaaaat ttaaatattc attaacttaa
     4261 tatgctttaa ttcacttcat taaagactac tcgctggacg tggtgttggc tttttcaaga
     4321 aactcataat cattattaca atgactaaca taccacaaat tagatacatc catttaaatc
     4381 ctactaaatc ggaaataggt cccatcaatg cgccacctaa cgatatacct aagtctgcac
     4441 aggcaataaa taaacctaac aacatattac gacctacttt tggtaagacg aagcttaagt
     4501 atgatgttaa tgttgggtac accattgctt gcgtcattcc tattaatatc gcactaccat
     4561 agaaaataat tgcacctact tgcggaccaa atgccactac aaatgacgcg attactaata
     4621 atgatagtac agataccata tatttaggat gccacatacc atctgacgga atgtatttcc
     4681 ttaagtaaaa tcttgccgca acaactgcga tggcctgtat tgtcaaaaag attcccgcat
     4741 tcgcgaatcc taaactcact gtgtataacg gtacaaatgt actaactgca ccaaatacaa
     4801 tcgatgcaac aatcatgata ataccactgt tcaacaactc tttatttttg aaaaattgcg
     4861 caaaaacagt tacagcgtta aacggcattt tttcaatctt atctgacgta tcgggttcct
     4921 gttcagcaaa ggtcacgcga tagccaaaga atgttgttgt taatgcgata aagataatga
     4981 caattgcaaa tagtgaaata ttatttgcat tccaaatacc tacggcaact aatggtccga
     5041 ttaagtttgg aatcgttgaa aatagcgagt acaatgatac accttcagaa cgatgttcct
     5101 ctggtaatgc atcaataata cctagctgta aagacataga aaagaatgcc gtacacacac
     5161 cttgcataac acgtgctacg aaataacctt ctaagccagt aaaaccataa ataattaaag
     5221 ctatggcatt gataatcaaa ataattctta atactttaat gggaccgaca cgagcaataa
     5281 tttgtcctgc ccatggtcga aataccattg ctgttaacat gtatgccccc ataacgatac
     5341 cgataaccgt attcgttgcg cctaaatcat gccctcgtaa aggtataaac acgtttaaga
     5401 ttgcattggc actaaagaac atcaatgtta atatatataa tctcaaaaag ggccaagcca
     5461 ttgcaccttt cattatctca ctccttagaa tgatagttgt ttttctatta attctcttgt
     5521 ataagcattg tcacttttat gcaatgcgct tgtcggaatt tgttcttcta tttttccgtt
     5581 tttaaaaata attaattgat cacataaata cgtggcagct tgaatatcat gtgtgataaa
     5641 aatataactc aactgacgcg tttcacgtaa atgaatcaat aaatctaata tttgtgtttg
     5701 aattgacatg tcgagtgaac taatggcttc atcaaacaaa atatatttag ggttaataca
     5761 tattgcacgc gcaatcgcaa cacgttgcgc ctctccacct gataacatat taggatattt
     5821 atccatgtat gccttagata gaccgacttc ttccaacaat gtaattgctt ggacttccat
     5881 aacttcttta ggttgtccat cacattgaca catcacttca aataagattt ctctaacagt
     5941 ctgaaatgga tgtaatgatg acgtataatc ttgaaataca gcaccaattt gatgacgtct
     6001 cactttcttc ttatgcatcg gttgatcatt taaggttaca caacctttat ccggtttctc
     6061 aatacctaat atcatacgac tcaacgtcga tttaccgcta ccactttctc cgataatcgc
     6121 aatcgtcgca ccgattggac actcaaatga cacacctttc acgataggtg ttcgacgacg
     6181 cttaaaaaca tgtgcgcttt gatatgactt ttcaacatct ttaattttaa tcatgtacat
     6241 cacccctcat cacatgttta aaatgatcat taatcttctt cttcgttgat aataaatact
     6301 tcgtataaac atgttctgga tgatgcaaga ctgattcacg tgtcccttgt tcaataagct
     6361 gaccattttt catcacaaca acacggtctg caatcttgtt aataaccgtt aaatcatgtg
     6421 aaatgaaaat catcgcacag tcaaagtgtt ttttaatatc tataaatgct tccagtacat
     6481 catattgtgt aattgtatct aaagccgttg tcggctcatc agcaatgatt aactttggtt
     6541 tcaaagctaa cgctaaagca atcatcaatc gctgtaacat tcctcctgat aacatgtaag
     6601 ggtatgattt taatatacgt ttaggatctt tcaaacttaa ataatccata tattcaatca
     6661 atgtcttttc aatttcttgt gtagacattg acgtatgtac tttcatagtc tcaaacattt
     6721 gtttaccgac agtagttgat gggtcaaagg cacgactacc ttgttgcatg accatcgcaa
     6781 tgtctttacc acggtacttt ttcaattgcg attcagataa tgacaacatt gatgtaccat
     6841 caaagataat ttcacctgtc accccgagtc gttcgggatt caaaccaata atcgatttac
     6901 aagtgattga tttaccacta ccactttctc caataacgcc taaagtttca cccttagtta
     6961 atgtaaaatt cacatcactc acgagtggtt gatctgtcca ggtatctgta atcgtcaaat
     7021 gtttaactgt taacaatgtc atgattgcac cactcctttt ttcacagaac gaagtttatc
     7081 tttagaagag atgcggggat caatagcaat ttgtaaagca tcggataaga agttaaatgc
     7141 catcactata atcactatgg caatacctgg cgcaaacatc atttcaggat gtgtaaacat
     7201 cacttttcta gcttcgttaa gcatcatgcc ccactctgca gtaggcgctt tgacacctaa
     7261 tcctaaaaat gaaaagccag atatttgcaa gatcattgaa cacatcgagc tactagagat
     7321 gatagcaata tctgctaatg ttaatggcat aatatgtttg tgaataattt tcatatcatt
     7381 cataccgatt gttttagcaa atcttacatg gtcagaagca gtgtactgca taacacttgt
     7441 acgtataaca cgacagaacc atgcccaacg cgtcaaaata aatgccatga taatattttc
     7501 ggcacccatt ccaaacaatg caattaatgc taacgttaca acataacttg ggaatgccaa
     7561 cataacatca cacgcacgca tgattaaggc gtcaacaaac ccttggaaat atcctgataa
     7621 gaatcctaaa atagatccaa taagtacaga aacaaatagc gcaacaaaga catataacaa
     7681 acttggtcta atcgcataaa ttaacctagt taaaatatct ctacctaaat ggtcagtacc
     7741 tagtagatgt tgaaaactca tgccagcaaa tttgtttgct gtatcgatat ggttaggatc
     7801 ataaaatgtc acaagtggtg ctgctaatcc taaaaagaca tataatacaa taatgcctaa
     7861 agcaattact gcacctttat cttgtaataa tcgttttaaa attatcatcg tgcgccctcc
     7921 cttaatcttg gatttaatag cgcattaatg atatctgcta atgtattaaa tacaataaat
     7981 aataccgcta caattaatac atatgcttga atgactggaa aatcgtgttc aagtattgct
     8041 tttaaactta attgacctag tccaggccat gcaaagatat actcgataac aactagtcca
     8101 cccattatca ttggtataga catacaaaag attgataccg caacttgtaa agcattacgc
     8161 aacacatgca acattaatgt gatagatttc acaccgcttg ctcttaaata aagtacataa
     8221 tcttcattta attgttccac catcgagcgt ctaacatttc taaagtaaat accagcatag
     8281 gcaatcgtaa taacgatcac tggcaatatg taactttctg gacctgttaa tccagaagtc
     8341 ggcaatatgt ttaacttcac tgaaacgtaa ataataagta ttgaagctat ccaatatgat
     8401 ggtaatgcag ttagaaagaa agccactgaa cgtatcgcac gatcagtgaa ctttcctctt
     8461 tttaatgcac taactacacc taaaataatt gatgtaatca tcaccataac acttgaaatt
     8521 attgttaatt tcaatgtatt cataaatgct ggaccaatac gttcagcaac tgggtcacct
     8581 gtaatgtagc ttgtaccaaa attaaattgc atcgcttcaa gtaaccaatt tttatattga
     8641 attaataatg gatcattgaa accgtacttc tcattcgttt ctgcaatcaa ctctggtgtt
     8701 acatttggcg tcccttgtgc atgtaaaatt gtcacagctg gattttcatt tgtaatatac
     8761 gtcaatagaa atgtcataaa acttactaca atcatcaatg gaaacatgag cgcaatacgt
     8821 tttaagataa atttgaacat ctaattgctc ctttatttat actgcatttc attgaatggt
     8881 aattcatact gtgattgtgt gaatgatact ttttctaaat cttttggtgc aacaactgtc
     8941 atactaccgt gtgaaatagg gataaagata ccttcatcat caatttgttt caaaatgttt
     9001 ttataagcgt ctgaacgctc tttaccgttt tggattttaa atgcgtcatc aatgctgttg
     9061 tatattttat ctttgttctc aatgcctgat gttgcacttt cataaccatt tttcgcttta
     9121 aatgctgcaa tagtactttg tggatcgtac aataatcccc aagtttggtt gaacattaag
     9181 tcataatcac cagaagtacg acgttcagca attttatctg atgtttcgcc attgatgttt
     9241 aacttaatac ccattttctt aaattctgct tgtaagtatt ctgcttgttc tttttgactt
     9301 gaagaacctt tgtcatagta cattgccatt tcaaggtttt taccatcttt ttgacgaaca
     9361 tcgctgtctt tacctttctt ccaaccagct tcatctaata atgattctgc ttttttaagg
     9421 tcatacttac gtgttggcat atcgaaatta atgtctgtta catttttcgc aaataattga
     9481 gttgctggtt tttcttgacc atctaaaatt tctttggcaa ttttatctct gtttaccata
     9541 tgaccaatcg cttgtctgac tgttttgtca ctcacagcgt tatctttttt accagaattg
     9601 acaactaaca ttttcgtatt cataggttga ctacgcttaa cttgatagtc acctgtatct
     9661 ttcaattgtt ttaaagagtc tttgtctaag ctatctgtac ctctatcatc tgtgaaggca
     9721 aagttcgttt cacctttttt cattgatagg aatgctgttt caccagcagg cattactttt
     9781 gcttgtactt tgttaagttt agacttttcg ccccagtatt gatcattttt gttaaagtct
     9841 gcagactcat cttttttgtg ttcacctaat ttaaatggac cagtaccatc gaacttttta
     9901 acgccatctt ttgttgtacc gtttttaaag tcttttggag acacaaatac atatggacga
     9961 ggcatcgcta attcagccaa tgcaggttga tatgcttctt tcaaattcaa ttcaaccgtg
    10021 tacttatctt taactttaac attgtcaatt aatgtcgaaa tctttaacca agaatgcaat
    10081 tttttgtttt cttgaactgc gtcaatattt ttcttaactg cgtcagcatc aaatggcgta
    10141 ccatcatgga atttaacgtc atctctcaaa tggaacgtgt atgtcttccc atcttcagac
    10201 acatcccact ttttagctag taaaggctta ataccatctt tcgtgttacg tacaagcggc
    10261 tcgtatatca tactttcagc agacattgat ccaccgtaaa catgcggatt catatcaccg
    10321 atatctttaa ccgtcgtata cgttaattgc ttgttttctt ttttctcctc taaaccttta
    10381 ttaccgccac aaccagttaa aattagccct gatgcaagta acattgcact cattttagtt
    10441 agttttctca tttgcttttc ctctttctaa attgataagt tgcattactt aatcaaatcg
    10501 taatgattat ttttctcaat tatttgctta tttttatgta aatgtcaaac ataattcaat
    10561 aatttttttc tattataaaa cttcaaaccc ttattatgaa agcgttctat tgatttccaa
    10621 aaattttgtg acgagtgctt tatctccttc aaatagattt gtattaaatt gagaagataa
    10681 gtgttgatct tgatgcatat ccttgtacgc ctggcaactt gcttcatagc gagttaggaa
    10741 ctgatcaatc attggcgttt tgatacctag cattttccca atatgctgaa tcatcgccgt
    10801 tctgtaataa tcttcacttg gcattcttgg aatttgaaca acatcctgtt catttttata
    10861 gacttgctta aatggtacag ctgaaaaatc aaagtaatgt ccgttttcgt ctggctgtga
    10921 aaatggatca atgaggattg cggtatatct tacataaagc agatattctt gtaagatatc
    10981 tggcaagatt tcgaaatgct caatatcacc ttcatccaaa gtttcaggac gtactggata
    11041 attttccttc accataaatt gaagcaggtt gactgacggc actctaaatg cttgtaaaat
    11101 aaccatcatt tccttccaca ttaaacgcat ttcacggatt agtgtcatcg ttatcggtcc
    11161 ttcaggaaat aacttataca cataaaccgg tacatctgtt ccttcgaaaa tggctttcaa
    11221 tgaaaagtca ttcataaata gtggtgggtg cacataaagc gaactgtttc gcgtttcagc
    11281 atgcagtggc gattcaacga cttctaattg aatcttcaat tgctcagcta aagcagagat
    11341 tcgttgacac attgttgagt ttgaatgtgt cgatcccatg tacaatttct tttttacacc
    11401 tgttgtcaac acatgattag gcgcttcttt atcaacaata cgtgtatcgc caagataagt
    11461 tgagaatgaa atcacttcga tatctttatt aaatttagac ataaattgtt cgacaatcat
    11521 ttgcgaacca aatgtcggtg atattaaaat gacatgtttg acactttgca aagtttctaa
    11581 cgacaattgc tgtagtgtgt cataataagc atctgctgtg catgccatga caaccgtttc
    11641 gtattcaccc ttaacgtttt taacatcttt atacaaacga ttaatttcaa acttaccttc
    11701 cagatgttga tgcgcctcgt tttgtatttt gacttcaaat tgtttcgctt ttttatacgc
    11761 ttgatataag cgttttgatt tttctgatgt tgaggcacgt ccaaccatat caatctcata
    11821 atctgatttt aaatagcaaa tattcgctaa ttggattgcg accggaccag tgcctatcat
    11881 taataattta gacatccgta atacctacct ttatagctgc ttttttataa agtgctatat
    11941 caaaaatttg ctgtggtctc atatgtttat tcacacattg ccacttatct tccgctgttt
    12001 cttgtgacgg ataattaaat attgctttga tgccatcacc aaagcgcatt gcaactacga
    12061 cattttcatt tgttaaatca tataattctt ctaaaatgct gtactttaaa ggaattgtcg
    12121 agctgaatat gatatgcgtc acatctttga tatctttaag ttcagatacc ttttgatccg
    12181 taattgttat atcttcattt ggtgctaaga cgttaacgat tctgcgccct aggtcaacgg
    12241 cttgtggatc aatatcaata ccgataactg aagcacctgt ttcttttgct acttgaatta
    12301 acgtcattgg atatgcacct gaaccaacta acaataattt gtcattgcta gctatactac
    12361 attgtccaaa ctcttcatca atacaatgtt ctatattgtc aaagtaacct gtggtagacg
    12421 cctgtccgtc taatagacgt ctcgctctaa tgacttccac ttgtttaaca cactgcgctg
    12481 taatatattg cagtgatttt attaattgtg ctttttcttc aacgtcacgc caagcattaa
    12541 attgttgttc ataaatagga tttaaaataa attcactata gtcatccatc aatgtttcta
    12601 atgcttcgat atattgatcg tcttgtaata cacgctcgta atgcgcttca aacttttcta
    12661 aatattgttg taatatcaat ttgatttcat tattaaagtt attcattgta ggctccttat
    12721 tctatatatg cttttcctgt agctacagtt gtaacctgtc ctttaattga agtttgatat
    12781 cccaattgat gacatcgctt tgatgtcact aaaatactgc cccctggctg atgtactgta
    12841 aaatctttgc atgcgtcatt acgttgataa ttattaaaaa ccccaattga tgctgtacca
    12901 gaaccacagc tattttccca aattaaactt tgaatttctg gtatatagat taatggctgt
    12961 aaaaattgac gttgttcatc aaaaagcatc atacctactg ttttatattt gtgactccat
    13021 tgttgctcac gcacaaacgc ttcaaccaaa tgttgaattt ctgttgttac ttgtttaact
    13081 ggaatcacat aatgtacata tgtttcataa ataatttcta ttgctttcca tgaatgatta
    13141 cccatattaa ttgttgttgg cacaacacga tgggcttgtg gcatttgaac ttcatagtat
    13201 tggcaatcat gaattgcgca ttgcactaaa tccgaacagc cagacacctt caccttaaac
    13261 tgttggtctt taagcaaatg actttcctgc aaatgatgta tatatgacat cgtcgcatta
    13321 ccgcaaaatt cattaccgct cataactaag tgaaaatcat taccatcatc attttgtgtt
    13381 gattctataa agcctacctg ttcacagcat acatgtgttg cggccatcaa ctgattggcg
    13441 atagatgcat attcactagc atcatgtttt gaatgaacaa gtatcgtcat attccccgaa
    13501 ggattatact tagaaaattc tataacctgc cgattcatac gacactcctt tagatgtatt
    13561 tttagagcct ctgtaatttt aattactaga ggctcttatg cagttgttgc gaagcaaaca
    13621 actgtattcc tctaaacctg taatttttta gaagatgaac ctaatagctc tatctagtag
    13681 gtaagtcatg ctaattcgca aaagcgaaaa ccatggctct tatgcagttg ttgcgaagca
    13741 agcaactgta ttcctctaaa actgtaattt tttagaagct gaacctaata gctctatcta
    13801 ttaggtaagt catgctaacc cgcaaagtgg aaagcatgac ttagtaatcg gttgttgtat
    13861 caaaatgcaa ccgtaaacct tatttaaagt tgcttcttgt ggctctacaa gcgaatgcag
    13921 ttgtccgtta actacattca cttagatgct tttactgttt tcacctcaac taaaacttca
    13981 aaaaagttgc agaaaacaaa tcgtaactat tacgatttga ttatatacta tattagttga
    14041 gaatcattgt caataaaggt tgtaaatgaa tgcacattta tattttgata atccttccat
    14101 aaacttcgta ttctcagcac attcaagtct ttttgaaaat ttttatcaat aattgagtag
    14161 aagttccgtt ctagtcagtt caaaatttta cgtaagttgt ctctgcgtat tgctttagtg
    14221 agaataaccc acacttgtgg aaacgtcctt ttgttaatct acaatggata cccttgtctt
    14281 tcgctaacat ttcctattac caatcctgta acggattttc atcgtcaagt tgttgcccat
    14341 acaatacgca ccaaaaaagc tttgtacaat ttcgtacaaa gctttaaaaa tttaaataaa
    14401 aatatcattg ttttaatgaa ctagccatat cagattttat ttcgtttgct aatacttgta
    14461 attcagggaa gtaattatta gcaaagccaa ttattatttt attttttaca aacgtgttaa
    14521 tctctcgatc ttctggaatc gtattttcta ttaaattcaa aagctcacta tgacttatgt
    14581 tctctatact tactgtattt aaatatttta aaatcacaag atacaattca tcaaatactt
    14641 catgttcaaa atcaaagtcc aataataata aactaacgga attagtttct aactgattat
    14701 ctccaatttt atctgcaatc tctcttatag cttgatatag caatctaaaa ctttcttttt
    14761 cttttaacat aaaattctcc ttaatttatt agaatagata aaatacagct tattttaaag
    14821 atactccaaa agtttgaact cgagattctg ataaattaga tgcacgacct acaatacgtt
    14881 aaaacaagct ttaagcaata cacttcaaat gaacaatatg tgattttcaa agattaatta
    14941 atctccgctt taaatgtctt tcattgcgac aatatgcata cattgcctac ttcatgccaa
    15001 attcttttcc actggatatc gattttaaat aattattcca atgtccgctt ccacgaatca
    15061 tcacttaaca tctcgccagg ccatgtgtaa gccatcacac caccatcaat tcgaatcgtc
    15121 tctccagtta tgaatgaact gtcgtcagat gctaagaaga ctactaattt agcaacttct
    15181 tctggtttac ctaaacgtcc cagcggagtc atccattttt gattttctct aaatgtttta
    15241 cctgcatcat cctcactcgt acctgtcagt ttatctacta acggggtttc aattgtacct
    15301 ggtgcaatcg cattggctcg aatgctatca cggccatact caattgcgat tgattttgta
    15361 aaattaatca ctgcaccttt cgcagcatta tatccagagc gatacaagtc tgctgcttgt
    15421 ccggaaaatg aagacgtatt aacaatagag ccaccttgat tcatcattaa aggtaacatc
    15481 attttcgtca ttaaaaatgt cccacgcata tctacattca taatcttgtc atacacatct
    15541 attggatact catgaattct atcagccgca ttatccacac cggcattatt aaacaacaca
    15601 tcgattcttc caaactgttc ctttatgtca gacacaaagt ctaccacttg ttgttcgctt
    15661 gcattatcca cattatacgc cttcgcattg tcaccattac ttttaatttt atcgacagtc
    15721 tccgataccg cttcagctat gtctaccgcc aatacatacg caccttattg agctaaagcg
    15781 attgcagaag cttgaccgat acctgtactt gctcctgtta cgactgctac tttattttcc
    15841 aaacgtttca ttcagtcact cctatacaac atatcttaaa cttacagtcc ttattttcca
    15901 ctgttcgatg cttcataaac ctaatagata agtcttcagg cagtcttaac tactctgcta
    15961 tacactttct attgaataac tcaaaatatt taacaatatt agttttttta ttttctgaaa
    16021 attgttactt ttgagagtat tttttgttac actttaaata tttaacaaag gggggaataa
    16081 ttgatgacat caaaacatca acaaaagggg tctatcgtca atcgtttctt gaatagtgtt
    16141 gaaaaaatcg gaaataaatt gccagatcct agcgtcttat tctttttaat gtgtgtgggc
    16201 ttagccatta tgacatgggt tatctcatta tttaatgtat ctgttaagca tccaggtacg
    16261 catcaaacca tttatattaa aaatataatt agccatgatg gatttacgat gataatgaat
    16321 gatacgatta aaaatttctc agagttccca gcattaggct tagtactagc agtgatgatt
    16381 ggtattggtg ttgcagagaa aacaggatac ttcgataagt taatgatttc tgttgtgaat
    16441 cgcgcaccac gcttcttaat tttaccgact attatattaa ttggtatttt aggtagtaca
    16501 gccggcgatg ctgcgacaat tatcttgccg ccgcttgcag caatgctttt tattaaaatt
    16561 ggctatcacc ctatcgctgg actaacgatg gcatatgctt ccgctgttgg aggatttgca
    16621 gcaaatatag ttgttggtat gcaagatgct ttggtctatt catttacaga accggcaaca
    16681 cgtatcgttt cagattctat taaaacaaac gttgctatga actggtactt tatcgcagcg
    16741 agtgtcgttg tattacttcc tacgatattg ctagttacaa ctaagctcat tattcctaga
    16801 ttaggaaaat atgatgacag tttaatgcat gatgaccatg aagaaacatc ttcacatatt
    16861 actgacaaag aagcacatgc tttaaaatgg gcaaatatca gtttcatagt cacaattatt
    16921 ttattaatta ttacagccat tcctgaacat agctttttaa gaaatgctaa aacaggcagc
    16981 ttactagacg atgcgccatt aattaacggt gtcggattaa ttattcttgt cgtattttta
    17041 gtacctggat tagtttatgg gattttaagc aaagaaatta aaaatacaaa agacttaggt
    17101 aaaatgttcg gcgatgctgt aggatcaatg ggaacattta tcgttattgt gttttttgca
    17161 gcacagctac ttgcgtattt aaagtggagt aatttaggaa ttatcgctgc tgttaaaggt
    17221 gccaaattat tagaacatca aaacggtatt gtattgatac taggtattat tgtactgagt
    17281 gcaatggtca acatgttaat cggaagcgca tcggctaaat ggggtatttt aggaccgata
    17341 ttcgtaccaa tgttaatcct tatcggcttt catcccgcat tcacacaagt catttaccgt
    17401 gtaggcgatt caattaccaa tccaattaca ccgatgatgc cgtacttacc tttattatta
    17461 acttatgcac aaaaatacga taaacgcatg aaactcggag ccttactttc tagtttaatg
    17521 ccgtattcaa tcgcgctaag tattgtatgg acagtatttg tcatcatttg gttcctatta
    17581 ggtatccctg ttggaccagg tggaccgata ttcgttaaat aacagtattc aaagggataa
    17641 attaaaatgc cactatacaa gatttgacct gtatagtggc atgatttgtt atgatatagc
    17701 gttcgagatg aaactctgca tttatatgaa caattatagt ctatcactaa taatatttaa
    17761 tctgttccaa ccgttaattt ggtttacaac aaagacaaga tcaataattt gttcttcatc
    17821 ataaaatgat tttagccggt caataatttc aaacttctta aagtctttga ttgaatttaa
    17881 catttctgca aattcaagcg tcactttttc ttgatcagta aataaatcta aatgtctaaa
    17941 actcaagact tcatcaattt tcatttgtgt tacacccaat tcctttaatt cttttttatg
    18001 gatatcaaca caataatgac aaccgttaat tttagaaatt aataacttaa tcaatgcact
    18061 aagttcatca ttaacgggtg attgatgaat aaacttttca ccttggctca ttacagacat
    18121 taactctcca ttttgtttct tgtaattata cggcatttgc aatctctcct ttacattgtt
    18181 taataccaat ccagtgatac tacacttcga acaatattgg atagaatttg aaattctgta
    18241 taacatcttg tcaatctatg ctttagttaa aacatttagc acttatattt aaaaaatata
    18301 taaaatatga aaacagattg aaaggatatt ataccttgct ctaccctttt ttataaaaaa
    18361 tcacacacat ttgagaaaat ttgataatgt caataaatca cgtcatcgac attgttattc
    18421 gatatatatt gttcttataa tggccaaact attattccat ttaaattaac gtggttcgtt
    18481 tgtgttaatg tactgtacaa aactggggaa gtattggtga tgatgaagtg atttatatta
    18541 attgcgaaac accttcgata gcaatgacat agattattgg aagattaaat caaaaataaa
    18601 gaagacccta gtggccttct tttttatcta attcttcttt gattttttct ggtgattttc
    18661 tttcatgtgc tggttttaat aatttatcta ctccttcact ttttatcata gcgccaccaa
    18721 attgataatt atgttcaggc gatgcataag cagtaaaatc atcttcttta ttttcattga
    18781 tgtatccttc aatcacagca cttcccatag gacttgtttc caagtttgtg aaatgtacag
    18841 atttaatcgt gttcgtttct ttagtattat gcttaagata gagtgtaata cgttcttgtt
    18901 gttctttgta gtaaatcgct ttttgttttt ctttttcatc atatttcatt ttaaaatgca
    18961 tgaccgtaga aactgtacat atacacaata tgacaccaat aattataaaa acatgttttt
    19021 tggacattaa agtaactcct aactttagtg actcttcttt ttatttagtt ctttttttat
    19081 atcatctggt gattttaact gattggctgg ctttagtaac tcgcttaatt tttcactttc
    19141 tatcatagca ccaccaaatt gataattatg ttctggtgta gcataagcag taaaattatc
    19201 ttttttgttt tcatttatgt aaccttcaat cacagcactt cccataggac ttgtttccga
    19261 gtttgtgaaa tgtacagatt tgattgtatt cggttcttta gtgttatgct tgagataaag
    19321 tgtaatgcgc gcttgttgtt ctttatagta tattgctttt tgtttttctt tttcgtcata
    19381 tttcactttt aaatagatac ctgcaacact aattaatatg attaacataa tactagttaa
    19441 tattaaaatt ttctttttcg acatctatca ccagctccta aaatgtggtg gaattttata
    19501 cagtttacat atttatgata ctacaaaaca cactcttaca aagaaaatct ataattttaa
    19561 tctaggtcaa ggaaaagcag ccaaaagtgt ttatataaga tcaacgaaat attgcgggta
    19621 taaagtggtt aacagataaa tacagtggaa attgtactaa aaattcaact tttcattttt
    19681 atatgtaagt atgattaact tgaaaacaaa attaaaacgg ccgcatagtt ttagcggtcg
    19741 ttctttttag actctaattc cttttttatc tcatcaggtg atttggtttg atgtactggt
    19801 tttaataacg tgcttaaatt tttacttttg attaaacttc caccaaattg ataattatgt
    19861 tctggagacc catatgcaac aaaatcatct tctttatttt catttatgta accttcaatt
    19921 actgcatcgc ccataggtcc tctttttaaa cttgtgaaat gtacagtttt gatggtattg
    19981 ggttctttag tattatgctt aagatagagt gtaatacgct cctgttgttc tttatagtat
    20041 attgcttttt gtttttcttt ttcgtcatat ttcactttta aataaatgac tgatgcaact
    20101 atacatatac ataaaatgac accaataatt ataaaaacat gttttttgtt cattaaagta
    20161 actcctaaaa tgtggtggaa aatgaaaaaa tttttataat ctataattat gaacattaca
    20221 gattataaac cataacacta acatcgtcgc cttcattaac ttgattgtta aaatcagcaa
    20281 ttactgagaa ttgtacaagg aataattgcc tattatgccc tcatgtaatt attgccttac
    20341 taacattaac aaaatcgata gctattacat taaatgccta taccccagac ctcaaacacc
    20401 tttttataca ggacgcactg taatttcatt cacgttaacg tgctttggtt gtgttaatgc
    20461 atataatact gcttctgcaa tatcttgtgg ttcaagtttt ttacgatctg ttggattgta
    20521 tgcggcagtt atggctgtat ctaccattcc tggagaaatg cttgttactt taacgcctgt
    20581 ctttgccaac tctttttcta atccttgagt aatagtgtga actgctgctt tcgtcgcact
    20641 ataaatcgta ctacttttcg ttacttcaaa gccagaaata gatgcaatgt taataagatg
    20701 gccacttgat tgttctaaca tagttggtaa tgcagcctgt gccgtatata aagtgccttt
    20761 gatattcaca tcaatcatac tatcccactc atctacttga taatcagtaa tcttagacga
    20821 caacatttgc cccgcactat tgataacaat atccaaacca ccgaatgttt gttgtgcaat
    20881 ttttatcaat tcatcgactt cttctttatt cgttacatct gttggcacta ccttcacact
    20941 atcttgtgac aattgattcg ctacgttttg taatttttct ttatttctac ctgctaagac
    21001 aacttttgcc ccttcttcat gtagtaatgt tgcaattgct tctccaatac cactacctgc
    21061 acctgtaact actgctactt tatctgttaa tactgtcata atgatcgact cctttgattc
    21121 tttttatttt ttcagggtaa atcataaata catattactt ttaaaaagcg tatcacaatt
    21181 catataacgg tcataataac tcgcttcatt ttcatagata aattacatta caagccattc
    21241 gaaacataca attaatcgtt gcttatattt tttattttta aaaatgttga aaaatcgtca
    21301 cttctttatt gtaaaaacat tatattagta ataaagttaa tactgtgatt tatcattcga
    21361 ttgaatgatt agagggagga ataaaacgtg acatatcatg agcgtgtttt agcattaaga
    21421 gcagaaagta aaagaaccgc atttgatttt cgattcgaag atttatttag caaagaagaa
    21481 tggctaagta tgtctcttgc agaaagacaa aaagctgaaa aagcatttcg acacgaagtt
    21541 aaaaatatgg acgatgtaag aatgcccttc tcaagtgtcc atgacgccca ag
//
LOCUS       DAKTIO010000028        20612 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.028, whole genome shotgun sequence.
ACCESSION   DAKTIO010000028 DAKTIO010000000
VERSION     DAKTIO010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20612
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.028"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(53..127)
                     /locus_tag="O0293_000798"
     tRNA            complement(53..127)
                     /locus_tag="O0293_000798"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(92..94),aa:Glu,seq:ttc)
     gene            complement(557..1840)
                     /locus_tag="O0293_000799"
     CDS             complement(557..1840)
                     /locus_tag="O0293_000799"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCY0279693.1"
                     /translation="MSSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQ
                     FGGETYKLHLVPSGIFYKDKLAVIGNGVVVDPVALLKELDGLNERGIPTSNLRISNRA
                     QVILPYHLAQDEYEERLRGDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLL
                     KSNIEYKQAYFKGMFNETCPSFDDIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVL
                     FEGAQGVMLDIDHGTYPFVTSSNPIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGP
                     FPTELFDEDGHHIREVGREYGTTTGRPRRVGWFDSVVLRHSRRVSGITDLSINSIDVL
                     TGLDTVKICTAYELDGKEITEYPANLDQLKRCKPIFEELPGWTEDVTNVRTLEELPEN
                     ARKYLERISELCNVQISIFSVGPDREQTNLLKELW"
     gene            complement(2118..3518)
                     /gene="dnaB"
                     /locus_tag="O0293_000800"
     CDS             complement(2118..3518)
                     /gene="dnaB"
                     /locus_tag="O0293_000800"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCY0279694.1"
                     /translation="MDRMYEQNQMPHNNEAEQSVLGSIIIDPELINTTQEVLLPESFY
                     RGAHQHIFRAMMHLNEDNKEIDVVTLMDQLSTEGTLNEAGGPQYLAELSTNVPTTRNV
                     QYYTDIVSKHALKRRLIQTADSIANDGYNDELELDAILSDAERRILELSSSRESDGFK
                     DIRDVLGQVYETAEELDQNSGQTPGIPTGYRDLDQMTAGFNRNDLIILAARPSVGKTA
                     FALNIAQKVATHEDMYTVGIFSLEMGADQLATRMICSSGNVDSNRLRTGTMTEEDWSR
                     FTIAVGKLSRTKIFIDDTPGIRINDLRSKCRRLKQEHGLDMIVIDYLQLIQGSGSRAS
                     DNRQQEVSEISRTLKALARELECPVIALSQLSRGVEQRQDKRPMMSDIRESGSIEQDA
                     DIVAFLYRDDYYNRGGDEDDDDDGGFEPQTNDENGEIEIIIAKQRNGPTGTVKLHFMK
                     QYNKFTDIDYAHADMM"
     gene            complement(3550..3996)
                     /gene="rplI"
                     /locus_tag="O0293_000801"
     CDS             complement(3550..3996)
                     /gene="rplI"
                     /locus_tag="O0293_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCY0279695.1"
                     /translation="MKVIFTQDVKGKGKKGEVKEVPVGYANNFLLKKNYAVEATPGNL
                     KQLELQKKRAKQERQQEIEDAKALKETLSNIEVEVSAKTGEGGKLFGSVSTKQIAEAL
                     KAQHDIKIDKRKMDLPNGIHSLGYTNVPVKLDKEVEGTIRVHTVEQ"
     gene            complement(3993..5960)
                     /gene="gdpP"
                     /locus_tag="O0293_000802"
     CDS             complement(3993..5960)
                     /gene="gdpP"
                     /locus_tag="O0293_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP phosphodiesterase GdpP"
                     /protein_id="HCY0279696.1"
                     /translation="MNRQSTKKALLIPFVIMIITAIVLMGVWFIFNSLIALIASIVLV
                     VMIIVSIILFRQALMKMDSYVDGLSAQISTTNNKAIKHLPIGIIVLDENDHIEWVNQF
                     MTDHMEANVISESVNEVFPNILKQLDRVKSVEIEYNQYHFQVRYSENDHCLYFFDITE
                     QVQTNELYENSKPIIATLFLDNYDEITQNMNDTQRSEINSMVTRVISRWATEYNIFFK
                     RYSSDQFVAYLNQKILADLEESKFDILSQLREKSVGYRAQLTLSIGVGEGTENLIDLG
                     ELSQSGLDLALGRGGDQVAIKSINGNVRFYGGKTDPMEKRTRVRARVISHALKDILAE
                     GDKVIIMGHKRPDLDAIGAAIGVSRFAMMNNLEAYIVLNETDIDPTLRRVMNEIDKKP
                     ELRERFITSDDAWDMMTSKTTVVIVDTHKPELVLDENVLNKANRKVVIDHHRRGESFI
                     SNPLLIYMEPYASSTAELVTELLEYQPTEQRLTRLESTVMYAGIIVDTRNFTLRTGSR
                     TFDAASYLRAHGADTILTQHFLKDDVDTYINRSELIRTVKVEDNGIAIAHGSDDKIYH
                     PVTVAQAADELLSLEGIEASYVVARREDNLIGISARSLGSVNVQLTMEALGGGGHLTN
                     AATQLKGVTVEEAIAQLQQAITEQLSRSEDA"
     gene            complement(5975..6913)
                     /locus_tag="O0293_000803"
     CDS             complement(5975..6913)
                     /locus_tag="O0293_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HCY0279697.1"
                     /translation="MGVAYVFSKIQPKATIIATIALVFVALALYLVPGLGLIFALFAT
                     IPGIVLWNKSIQSFGISALITVIITTVLGNTFVLSAIILVLIASLIIGQLLKERTSKE
                     RILYVTTVAMSLISLIAFMLLQTFGRIPPSASIVKPFKQTLHEAITMSGADANMTQIL
                     EEGFRQATVQLPGFIIIITFLIVLINLIVTFPILRKFKIATPVFKPLFAWQMSGILLW
                     IYIIVIICLLFTGQPSVFQSILLNFQLVLSLVMYIQGLSVIHFFGKAKGLPNAVTILL
                     LVIGTILTPTTHIVGLLGVIDLSLNLKRIMKNNSKK"
     gene            complement(7204..8172)
                     /locus_tag="O0293_000804"
     CDS             complement(7204..8172)
                     /locus_tag="O0293_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCY0279698.1"
                     /translation="MTNYTVDTLNLGEFITESGEVIDNLRLRYEHVGYHGQPLVVVCH
                     ALTGNHLTYGTDDYPGWWREIIDGGYIPIHDYQFLTFDVIGSPFGSSSPLNDPHFPKK
                     LTLRDIVRANERGIQALGYDKINILIGGSLGGMQAMELLYNQQFEVDKAIILAATSRT
                     SSYSRAFNEIARQAIHLGGKEGLSIARQLGFLTYRSSKSYDERFTPDEVVAYQQHQGN
                     KFKEHFDLNCYLTLLDVLDSHNIDRGRTDVTHVFKNLETKVLTMGFIDDLLYPDDQVR
                     ALGERFKYHRHFFVPDNVGHDGFLLNFSTWAPNLYHFLNLKHFKRK"
     regulatory      complement(8232..8330)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(8535..8864)
                     /locus_tag="O0293_000805"
     CDS             complement(8535..8864)
                     /locus_tag="O0293_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCY0279699.1"
                     /translation="MITHMNMLILILLCGIVTLLIRIIPFIMISKVQLPDVVVRWLSF
                     IPITLFTALVIDSIIQQTPHGEGYTLNIPYIIALIPTVILSIITRSLTITIISGIVIM
                     AALRFFF"
     gene            complement(8861..9556)
                     /locus_tag="O0293_000806"
     CDS             complement(8861..9556)
                     /locus_tag="O0293_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCY0279700.1"
                     /translation="MTTHLSFRQGVQECIPTLLGYAGVGISFGIVASSQNFSILEIVL
                     LCLVIYAGAAQFIMCALFIAGTPISAIVLTVFIVNSRMFLLSMSLAPNFKTYGFWNRV
                     GLGSLVTDETFGVAITPYLKGEAINDRWMHGLNITAYLFWAISCVAGALFGEYISNPQ
                     TLGLDFAITAMFIFLAIAQFESITKSRLRIYIVLIIAVIVMMLSLSMFMPSYLAILIA
                     ATISAALGVMMEQ"
     gene            complement(10206..11492)
                     /gene="serS"
                     /locus_tag="O0293_000807"
     CDS             complement(10206..11492)
                     /gene="serS"
                     /locus_tag="O0293_000807"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCY0279701.1"
                     /translation="MLDIRLFRNEPDTVKSKIELRGDDPKVVDEILELDEQRRKLISA
                     TEEMKARRNKVSEEIALKKRNKENADDVIAEMRTLGDDIKEKDSQLNEIDNKMTGILC
                     RIPNLISDDVPQGESDEDNVEVKKWGTPREFSFEPKAHWDIVEELKMADFDRAAKVSG
                     ARFVYLTNEGAQLERALMNYMITKHTTQHGYTEMMVPQLVNADTMYGTGQLPKFEEDL
                     FKVEKEGLYTIPTAEVPLTNFYRNEIIQPGVLPEKFTGQSACFRSEAGSAGRDTRGLI
                     RLHQFDKVEMVRFEQPEDSWNALEEMTTNAEAILEELGLPYRRVILCTGDIGFSASKT
                     YDLEVWLPSYNDYKEISSCSNCTDFQARRANIRFKRDKAAKPELAHTLNGSGLAVGRT
                     FAAIVENYQNEDGTVTIPEALVPFMGGKTQISKPVK"
     misc_binding    complement(11582..11798)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(11871..13385)
                     /gene="hutH"
                     /locus_tag="O0293_000808"
     CDS             complement(11871..13385)
                     /gene="hutH"
                     /locus_tag="O0293_000808"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HCY0279702.1"
                     /translation="MTLYLDGETLTIEDIKSFLQQQSKIEIIDDALERVKKSRAVVER
                     IIENEETVYGITTGFGLFSDVRIDPTQYNELQVNLIRSHACGLGEPFSKEVALVMMIL
                     RLNTLLKGHSGATLELVRQLQFFINERIIPIIPQQGSLGASGDLAPLSHLALALIGEG
                     KVLYRGEEKDSDDVLRELNRQPLNLQAKEGLALINGTQAMTAQGVISYIEAEDLGYQS
                     EWIAALTHQSLNGIIDAYRHDVHAVRNFQEQINVAARMRDWLEGSTLTTRQSEIRVQD
                     AYTLRCIPQIHGASFQVFNYVKQQLEFEMNAANDNPLIFEEANETFVISGGNFHGQPI
                     AFALDHLKLGVSELANVSERRLERLVNPQLNGDLPAFLSPEPGLQSGAMIMQYAAASL
                     VSENKTLAHPASVDSITSSANQEDHVSMGTTAARHGYQIIENARRVLAIECVIALQAA
                     ELKGVEGLSPKTRRKYDEFRSIVPSITHDRQFHKDIEAVAQYLKQSIYQTTACH"
     gene            13711..14523
                     /locus_tag="O0293_000809"
     CDS             13711..14523
                     /locus_tag="O0293_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HCY0279703.1"
                     /translation="METLNSINIPKRKEDSHKGDYGKILLIGGSANLGGAIMLAARAC
                     VFSGSGLITVATHPTNHSALHSRCPEAMVIDINDTKMLTKMIEMTDSILIGPGLGVDF
                     KGNNAITFLLQNIQPHQNLIVDGDAITIFSKLKPQLPTCRVIFTPHLKEWERLSGIPI
                     EEQTYERNREAVDRLGATVVLKKHGTEIFFKDEDFKLTIGSPAMATGGMGDTLAGMIT
                     SFVGQFDNLKEAVMSATYTHSFIGENLAKDMYVVPPSRLINEIPYAMKQLES"
     gene            complement(14610..17273)
                     /gene="gyrA"
                     /locus_tag="O0293_000810"
     CDS             complement(14610..17273)
                     /gene="gyrA"
                     /locus_tag="O0293_000810"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HCY0279704.1"
                     /translation="MAELPQSRINERNITSEMRESFLDYAMSVIVARALPDVRDGLKP
                     VHRRILYGLNEQGMTPDKSYKKSARIVGDVMGKYHPHGDLSIYEAMVRMAQDFSYRYP
                     LVDGQGNFGSMDGDGAAAMRYTEARMTKITLELLRDINKDTIDFIDNYDGNEREPSVL
                     PARFPNLLANGASGIAVGMATNIPPHNLTELINGVLSLSKNPDISIAELMEDIEGPDF
                     PTAGLILGKSGIRRAYETGRGSIQMRSRAVIEERGGGRQRIVVTEIPFQVNKARMIEK
                     IAELVRDKKIDGITDLRDETSLRTGVRVVIDVRKDANASVILNNLYKQTPLQTSFGVN
                     MIALVNGRPKLINLKEALVHYLEHQKTVVRRRTQYNLRKAKDRAHILEGLRIALDHID
                     EIISTIRESDTDKVAMESLQQRFKLSEKQAQAILDMRLRRLTGLERDKIEAEYNELLN
                     YISELEAILADEEVLLQLVRDELTEIRDRFGDDRRTEIQLGGFENLEDEDLIPEEQIV
                     ITLSHNNYIKRLPVSTYRAQNRGGRGVQGMNTLEEDFVSQLVTLSTHDHVLFFTNKGR
                     VYKLKGYEVPELSRQSKGIPVVNAIELENDEVISTMIAVKDLESEDNFLVFATKRGVV
                     KRSALSNFSRINRNGKIAISFREDDELIAVRLTSGQEDILIGTSHASLIRFPESTLRP
                     LGRTATGVKGITLREGDEVVGLDVAHANSVDEVLVVTENGYGKRTPVNDYRLSNRGGK
                     GIKTATITERNGNVVCITTVTGEEDLMIVTNAGVIIRLDVADISQNGRAAQGVRLIRL
                     GDDQFVSTVAKVKEDAEDETNEDEQSTSTVSEDGTEQQREAVVNDETPGNAIHTEVID
                     SEENDEDGRIEVRQDFMDRVEEDIQQSSDEE"
     gene            complement(17310..19244)
                     /gene="gyrB"
                     /locus_tag="O0293_000811"
     CDS             complement(17310..19244)
                     /gene="gyrB"
                     /locus_tag="O0293_000811"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCY0279705.1"
                     /translation="MVTALSDVNNTDNYGAGQIQVLEGLEAVRKRPGMYIGSTSERGL
                     HHLVWEIVDNSIDEALAGYANQIEVVIEKDNWIKVTDNGRGIPVDIQEKMGRPAVEVI
                     LTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSQDLEVYVHRNETIYHQAYKKGVPQF
                     DLKEVGTTDKTGTVIRFKADGEIFTETTVYNYETLQQRIRELAFLNKGIQITLRDERD
                     EENVREDSYHYEGGIKSYVELLNENKEPIHDEPIYIHQSKDDIEVEIAIQYNSGYATN
                     LLTYANNIHTYEGGTHEDGFKRALTRVLNSYGLSSKIMKEEKDRLSGEDTREGMTAII
                     SIKHGDPQFEGQTKTKLGNSEVRQVVDKLFSEHFERFLYENPQVARTVVEKGIMAARA
                     RVAAKKAREVTRRKSALDVASLPGKLADCSSKSPEECEIFLVEGDSAGGSTKSGRDSR
                     TQAILPLRGKILNVEKARLDRILNNNEIRQMITAFGTGIGGDFDLAKARYHKIVIMTD
                     ADVDGAHIRTLLLTFFYRFMRPLIEAGYVYIAQPPLYKLTQGKQKYYVYNDRELDKLK
                     SELNPTPKWSIARYKGLGEMNADQLWETTMNPEHRALLQVKLEDAIEADQTFEMLMGD
                     VVENRRQFIEDNAVYANLDF"
     gene            complement(19254..20366)
                     /gene="recF"
                     /locus_tag="O0293_000812"
     CDS             complement(19254..20366)
                     /gene="recF"
                     /locus_tag="O0293_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCY0279706.1"
                     /translation="MKLNTLQLENYRNYDEVTLKCHPDVNILIGENAQGKTNLLESIY
                     TLALAKSHRTSNDKELIRFNADYAKIEGELSYRHGTMPLTMFITKKGKQVKVNHLEQS
                     RLTQYIGHLNVVLFAPEDLNIVKGSPQIRRRFIDMELGQISAVYLNDLAQYQRILKQK
                     NNYLKQLQLGQKKDLTMLEVLNQQFAEYAMKVTDKRAHFIQELESLAKPIHAGITNDK
                     EALSLNYLPSLKFDYAQNEAARLEEIMSILSDNMQREKERGISLFGPHRDDISFDVNG
                     MDAQTYGSQGQQRTTALSIKLAEIELMNIEVGEYPILLLDDVLSELDDSRQTHLLSTI
                     QHKVQTFVTTTSVDGIDHEIMNNAKLYRINQGEIIK"
     gene            complement(20363..20608)
                     /gene="yaaA"
                     /locus_tag="O0293_000813"
     CDS             complement(20363..20608)
                     /gene="yaaA"
                     /locus_tag="O0293_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HCY0279707.1"
                     /translation="MIILVQEVVVEGDINLGQFLKTEGIIESGGQAKWFLQDVEVLIN
                     GVRETRRGKKLEHQDRIDIPELPEDAGSFLIIHQGEQ"
BASE COUNT         6235 a         4134 c         3129 g         7114 t
ORIGIN      
        1 ctcctgcgtg acaggcaggc gtgttaaccg ctacactacg agacctaaaa aatggtgact
       61 cctacgggac tcgaacccgt gttaccgccg tgaaagggcg gtgtcttaac cgcttgacca
      121 aggagccgtt cttaagcaca agatagatta tagcacagcc aatcttgcca tgacaagata
      181 ttttttacat aattttgcta ttattttttg aagaatgtta taagtgaaaa attgcatttt
      241 attggcagtc tattcattat aataattacg ctatttttga aatttttcta ttttaggtgc
      301 tttcatttac acttacgtct actctatttt aatagctatt ttcgacatca gacttcttac
      361 attcaagtat ttcatcttaa ttttcgttaa atatcatcta atcttctaaa ttatgactag
      421 caaatatcgc ctttacgtta gtatttttta tctaaataat cgaatcgata cagaagcaag
      481 cataacaaaa gcctcctacc aataaagata gaaggctcat gtatttataa tcattgtgta
      541 tgacttatat aaagttctac cacaattctt ttaataggtt tgtttgttct ctatctggac
      601 caactgagaa gatagaaatt tgtacattac ataattctga aatacgctct aaatatttac
      661 gtgcattttc aggtaattct tctaaagtac gcacatttgt tacgtcttct gtccaacctg
      721 gtaactcttc aaagattggt ttacaacgtt ttaattgatc taagtttgct gggtactcag
      781 taatttcttt accgtctaat tcataagctg tacagatttt cactgtgtct aggcctgtta
      841 aaacatcgat tgagttaata gataaatctg taataccact tacacgacga gagtgacgta
      901 atacaactga atcaaaccaa cctacacgac gtggacgtcc tgttgttgta ccgtattcac
      961 gaccaacctc tctaatatga tgtccatctt catcgaataa ttcagtaggg aatggaccat
     1021 caccaacacg tgatgtataa gctttacata caccaattac ctttgaaacg aatgtaggac
     1081 ctacacctgt accaacagta acgttacctg caattggatt acttgatgta acgaatggat
     1141 atgtaccatg gtcgatatct aacattacac cttgcgcacc ttcgaaaagt accttttcat
     1201 ctgctacaaa tgcatcgtct aagatttttg atgtgtctgt tacaaattct tttagacgtt
     1261 gacctgctgc ataatattct tcaaagatat catcaaatga tggacatgtt tcgttaaaca
     1321 tacctttgaa atatgcttgt ttatattcaa tgtttgattt taataatctt tcgaatgttt
     1381 ctttttcaag taaatctgcc atacgaatac cgatacgttg aactttgtct acatatgctg
     1441 gaccgatacc ttttttagtt gtaccaatct tattgtcacc acgtaaacgt tcttcatatt
     1501 catcttgtgc taagtgatat ggtaaaatca cttgcgcacg attagatata cgtaaattac
     1561 ttgtaggaat gccacgttca tttaatccgt ctaattcttt caatagtgca actggatcaa
     1621 caacgactcc gttaccgatt accgctaatt tgtctttgta aaagatacca gatggtacta
     1681 aatgtaattt atatgtttct ccgccaaatt gaatggtatg gcctgcatta ttaccacctg
     1741 aaaaacgcgc gataacatct gactgttctg ccaagaaatc cgttattttt ccttttcctt
     1801 cgtctcccca ttgtgtccca actactacga ttgatgacat gtgagcacct ccaagttttt
     1861 ctcatttaac catttgatat tctaccaata tcgagaccat aatgcaaata aaaatcgaac
     1921 attgattttc ggccttttta aaagaaaaat cattttaaca aatcgttttc aacgtcctaa
     1981 aaacccttta acagaggcgt tgacagtact aatttatcac tttcaataca caaacttaac
     2041 atatatcaac ccgttcagaa caaaataaaa accgtacaat tttatcatct taatgattat
     2101 tgtacggaaa aactttttta catcatatct gcatgtgcat aatcgatatc ggtaaattta
     2161 ttatattgtt tcataaaatg taacttaact gtgcctgttg gaccgttacg ttgcttagca
     2221 atgataattt caatttcacc gttttcatca ttcgtttgtg gctcgaaacc accatcatcg
     2281 tcatcatctt catcgccgcc acggttatag taatcatcac ggtataagaa tgcaacgata
     2341 tcggcatctt gctcaatcga accagattca cgaatatcac tcatcattgg acgtttatct
     2401 tgtcgttgtt caacaccacg agataactga cttaatgcga taactggaca ttctaattca
     2461 cgggctaatg cttttaatgt acgagagatt tcagaaactt cctgttgtct gttatcggac
     2521 gcacgtgaac cactaccttg aatcaactgt aagtagtcaa tcacaatcat gtctaagcca
     2581 tgttcttgct ttaatcgacg acatttagaa cgtaaatcat taattcgaat acccggtgta
     2641 tcatcaataa aaatcttcgt acgtgataat ttacctaccg ctatagtaaa acgactccaa
     2701 tcttcctcag tcatagtacc cgttcttaag cggtttgagt caacatttcc agaactacaa
     2761 atcatacgtg tggctaactg atcagcaccc atctctagcg agaaaatacc aactgtatac
     2821 atatcttcat gcgttgcaac tttttgtgca atattaagtg cgaacgcagt cttacctaca
     2881 gatggacgcg ctgcaaggat aattaaatca tttcggttga accctgctgt catttggtct
     2941 aaatctcgat atcctgtagg tatacctggt gtttgaccac tattttgatc aagctcttca
     3001 gctgtttcat acacttgtcc taagacgtct cgaatgtctt taaagccatc gctttcacga
     3061 gaagatgata gctctaaaat tcgacgttct gcatcactta aaatcgcatc tagttcaagt
     3121 tcatcattat atccatcatt ggcaatacta tctgcagttt gaatcaatct acgttttaat
     3181 gcatgcttag aaacgatatc agtataatac tgaacatttc gcgtcgttgg tacatttgta
     3241 gataactctg caagatattg cgggccaccc gcttcattca acgtaccttc cgtcgataat
     3301 tgatccatca atgttacaac atcaatttct ttattatctt catttaagtg catcattgca
     3361 cggaaaatat gttgatgggc acccctataa aacgactcag gaagcaaaac ttcctgagta
     3421 gtattaatca attctggatc tataataatt gaacctaaga cagactgttc agcttcattg
     3481 ttatgcggca tttgattttg ctcatacatt ctatccatga atggttacac ctcttatttc
     3541 aatccaactt tattgttcaa ctgtgtgtac gcgaattgta ccttcaactt ctttatctaa
     3601 tttaacaggt acattcgtat atcctaggga atgaattcca tttggtaaat ccattttacg
     3661 tttatcaatt ttaatatcat gttgtgcttt tagtgcttcg gcaatttgtt ttgtacttac
     3721 tgacccaaac aatttaccac cttcaccagt ttttgctgat acttcaactt caatgtttga
     3781 taacgtttct tttaatgctt tagcatcttc aatttcttgt tggcgttctt gttttgcacg
     3841 ttttttctgt aactctaatt gtttaaggtt acctggtgtt gcttctacag cataattctt
     3901 tttcaataag aagttatttg cataacctac tggtacttct ttaacttcac cttttttacc
     3961 tttaccttta acatcttgtg taaaaattac tttcatgcat cttcactcct acttaattgt
     4021 tctgtaattg cttgttgtaa ttgtgctatc gcctcttcga ctgtcacacc tttaagttgt
     4081 gttgccgcat tggttaaatg tccaccgcca ccaagtgctt ccattgttaa ctggacattt
     4141 actgaaccga gtgaacgcgc agatatacca atcagattat cttcacgtct cgcaacaaca
     4201 tatgatgctt caataccttc taaacttaac agttcatctg ctgcttgtgc aactgttact
     4261 ggatgataaa ttttatcgtc tgaaccatgc gcaatggcta tgccattatc ttcaactttt
     4321 acagttcgaa ttaattcaga tcgattaatg taagtatcca catcatcttt taagaaatgt
     4381 tgcgttaaaa tcgtatctgc accatgtgca cgtaaataac tcgctgcatc gaatgttctt
     4441 gatcctgttc gtaatgtaaa gtttcttgta tctacaataa tacctgcata catcactgtt
     4501 gattcaagac gtgttaaacg ttgttctgtt ggttgatatt ccagtaactc tgttaccaat
     4561 tcagctgtcg aacttgcgta tggttccata tatatcaaca atggattaga gatgaagctt
     4621 tcaccacgtc tatgatgatc gataacaact ttacggtttg ctttatttaa gacattttca
     4681 tctaaaacca gttccggttt atgcgtatca acaatcacta cggttgtctt agatgtcatc
     4741 atatcccaag catcatctga tgtaataaat cgctctctta actctggctt tttatctatt
     4801 tcgttcatca cgcgtcgtaa tgttggatca atgtcagtct catttaatac gatgtatgct
     4861 tctaaattat tcatcattgc aaatctagac acaccgattg ctgcaccaat tgcatctaag
     4921 tcaggacgtt tatgtcccat gataatgact ttgtcaccct ctgcaaggat atcttttaac
     4981 gcatgtgaga tcacacgtgc tcttacacga gtacgtttct ccatcgggtc agtcttaccg
     5041 ccatagaaac gcacattacc attaatactt ttaattgcaa cttggtcgcc accgcgtcct
     5101 aatgctaagt ctaggcctga ttgtgataat tcacctaagt cgattaaatt ttcagtacct
     5161 tcaccaacac cgatacttaa tgttaattgg gcacgataac caacactttt ttcacgtaat
     5221 tgactcaaga tatcaaattt agattcttct aagtcagcta atattttttg atttaaatag
     5281 gctacgaatt gatcggaact gtatcttttg aaaaatatat tatactcagt tgcccatcga
     5341 ctaatgacac gcgttaccat tgagttgatt tccgaacgct gcgtatcatt catattttgc
     5401 gtaatctcat cgtagttatc taaaaataat gtcgcaatga ttggtttaga attttcatat
     5461 agttcatttg tttgtacttg ttcagttata tcaaagaaat agaggcagtg atcattctca
     5521 gaataacgta cttggaaatg atactgatta tattctattt caacggattt cactctatct
     5581 aattgcttta aaatgtttgg aaatacttca tttacagatt cagaaatgac attcgcttcc
     5641 atatgatctg tcataaattg gttaacccat tcgatgtgat cattttcatc taaaacaatg
     5701 ataccaattg gtaaatgttt gattgcttta ttatttgttg ttgaaatttg agcactcaaa
     5761 ccatctacat aactatccat tttcattaaa gcttgtctga ataaaatgat gctaacaata
     5821 atcatcacga caagaacgat agatgcaatt agtgctataa gactattaaa gataaaccat
     5881 acacccatta aaacaattgc tgtgatgatc atgatgacaa atggtattag taaagctttc
     5941 ttagtggact gccgattcat tattccacct ctattcactt tttagaatta tttttcatga
     6001 ttcgcttcaa attcaaactt aaatcgataa caccaagtag tcctacaata tgtgtcgtag
     6061 gtgtcagtat tgtaccgata accaatagta aaatcgttac tgcattcggc aaacctttcg
     6121 ctttaccaaa gaaatgaata acacttaaac cttgaatata cattactaat gataacacaa
     6181 gttggaagtt taaaagaatg ctctggaaca cactcggttg acctgtaaat aataaacata
     6241 tgataacaat aatgtatatc cataataaaa taccgctcat ttgccacgcg aaaagtggct
     6301 taaatacagg tgtagcgatt ttaaattttc gtaaaatcgg aaatgtaacg attaagttaa
     6361 ttaagacgat taaaaatgta atgataatga tgaaacctgg taattgaacg gtcgcttgtc
     6421 taaacccttc ttctaatatt tgggtcatat tcgcatcggc accgctcatc gtaatcgctt
     6481 catgtaatgt ttgcttgaaa ggttttacta tgctcgctga tggtggaatc cttccgaatg
     6541 tttgtagtaa cataaaagcg attaatgaaa ttaagctcat cgctactgtt gttacgtata
     6601 atattctttc tttagacgtt ctttctttga gcaattgacc aataattaaa cttgcaatta
     6661 agactaatat gatggcactt aaaacgaaag tattacctaa aacagttgtt ataattactg
     6721 taataagtgc actaatcccg aaagattgta ttgatttatt ccataaaacg atacctggta
     6781 tggttgcaaa taatgcaaat attagtccta aaccaggcac tagatataaa gctaaagcga
     6841 caaataccaa cgcaatcgtt gcaattattg ttgctttagg ttgtattttt gaaaacacat
     6901 aagccactcc catattttta actatagcta ttattttaac ctctttaatg aaaattaaca
     6961 atttatagat tgtatgcttc tatttcattt aattgaataa taactttcat gttttataag
     7021 taattaacat actcatttga atcgcttttg tgtgctttca ttttcaacat gattatttaa
     7081 tcccactaca tagcaatcaa gcttgattta gatttacaat acatttccac tctcatgtac
     7141 tctagatgtt tttgaatatg ataactgtga tttagtggct tcattctttg aaaatatata
     7201 ttattactta cgcttaaaat gctttaaatt taagaaatga tataagttag gtgcccaggt
     7261 actaaagttt agtaggaatc catcatgccc aacattatca ggcacgaaga aatgacgatg
     7321 atatttaaaa cgttcaccta atgcacgaac ttgatcatcc ggatatagca aatcatctat
     7381 gaaccccatc gttaacactt ttgtttctaa atttttaaaa acatgcgtta cgtctgtgcg
     7441 acctcggtca atgttgtgac tatccaatac atctagcagt gtcagataac aattcaaatc
     7501 aaaatgttct ttaaatttat taccttgatg ttgttggtat gcgactactt catccggcgt
     7561 gaaacgttca tcataacttt ttgatgatcg atatgtcaaa aaacctaatt ggcgtgcaat
     7621 acttagacct tccttaccac caagatgaat ggcttgcctt gcaatttcat tgaaagctct
     7681 actataagat gatgttcgac ttgttgcagc aaggataatg gctttatcta cttcaaactg
     7741 ttgattgtag agtagttcca ttgcttgcat acctccaaga cttcccccta ttaaaatatt
     7801 aatcttatca taaccaaggg cttgtatacc tcgttcattc gctctgacta tatctcttaa
     7861 tgttaatttt ttaggaaaat gagggtcgtt taaaggtgaa cttgaaccga aaggactacc
     7921 aataacatca aatgttaaaa attgataatc gtgaatgggt atatatcccc catcaataat
     7981 ttctcgccac caacccggat aatcatctgt tccatatgtt aaatgattgc cagttaatgc
     8041 atgacaaact acaactaatg gttgtccatg ataaccgaca tgctcatatc tcaaacgcaa
     8101 gttatctatg acttccccag attctgtaat aaattcccct aaatttaaag tatctactgt
     8161 gtaatttgtc attgttcttt cctccttaaa caaaaaaact tctcacccta ttgaaaagta
     8221 agaagtcttt atacttatca ttcgagtaac tcgttggttt tagcaccgtg ctataaagtc
     8281 ggttgctgaa gtatcacagg gccaagtccc tcaacttctc acaataagaa atatgaaatt
     8341 gttatgtgtt agttgagatt cagtgatgaa ttacttttat catttaaaat gttgttatca
     8401 ttgtcatgcg ttagccaaat cgcttacgta tacacgattc ccaatcttaa catagacgat
     8461 ttgtatatca gaattttctg attactaaca gtttacctaa gtttaaatat ctgttcaatg
     8521 attttcagtt atttttaaaa gaaaaatcgt aatgctgcca tgataacaat cccactaata
     8581 attgtaatag ttaaactacg cgtgattata gataaaataa ccgtcggaat gagcgcgata
     8641 atgtaaggga tgtttaatgt atacccctca ccatgaggcg tctgttgaat aatgctgtca
     8701 atgacaagtg ccgtaaatag tgtgattggg ataaatgata gccatcgaac cacgacatca
     8761 ggcaattgca cttttgaaat catgataaaa ggtataattc gaattaatag cgttacgata
     8821 ccacacaata aaataagtat taacatgttc atatgagtta tcattgttcc atcatcactc
     8881 ctaacgctgc tgaaattgtg gctgcaatta atattgctag atatgaaggc ataaacatac
     8941 ttagcgataa catcattact atgacggcaa taatgagtac tatgtaaatt cttaatcgcg
     9001 atttagtaat tgattcaaat tgcgcaatgg ccaaaaagat aaacatagcc gtgatagcaa
     9061 aatctaaccc tagcgtttgc ggatttgaga tatattcgcc aaataaagcc ccagctacac
     9121 atgaaattgc ccaaaataaa tatgctgtga tgttaagacc atgcatccaa cgatcattga
     9181 tagcttctcc ttttaaataa ggtgtaatgg cgacgccaaa cgtttcgtca gttactaatg
     9241 aacctaatcc aacacggttc caaaacccat atgtcttgaa gtttggtgca agcgacatac
     9301 ttaaaaggaa cattcttgaa tttacgataa atacagttag tacaatcgct gatataggtg
     9361 tacctgctat aaacaacgcg cacataataa attgcgcagc accggcatat ataacaagac
     9421 ataacaagac aatttctaaa atactaaagt tttgagacga agccacaata ccaaatgaaa
     9481 taccaacacc ggcataaccc aataatgttg ggatacactc ttgcacgcct tgtctaaaac
     9541 ttaaatgtgt tgtcatctca attacctcct ttgccacttt atatttatat aattacgaaa
     9601 aaataatata cctagtctaa tacagttcta taccctaagc aataacatta gacatcagtt
     9661 tgtctgaggt tagacatacc ggagtcttta gtcagcttca tattaacttt ttatttttga
     9721 gaattttcaa ttttttattt aagactacct ccatattttc tatggatttg tagttgtttt
     9781 taagtatcaa ttttataaat ttttatatct gatgatgagt ctgggatatt gattcatgta
     9841 ccactccctt ataatcatcc cctcccccta ccctactcca tcgatataac tcatactaca
     9901 tatcaacgaa atcagtattt tatcgcttcc tttcctatat tggtgatgct caaacttgtt
     9961 acgttttaga ttgttttagt tcatcataat tatcccgtat tgttgctata atgaaatgcg
    10021 ttcaccccat taaaccacaa acttaattta ttgttgttat gtgcattggc tcactattat
    10081 acttttacag cacaaaaaaa gtggcgacag cttcgtcacc actttttaaa atattattta
    10141 aagtatcttg cccttgcttt aagtatacgt agatatatac tttttaaagc ttgtagctaa
    10201 agcctttatt taactggttt tgaaatttgt gttttaccac ccataaatgg tactaatgct
    10261 tctggaattg ttactgttcc atcttcattt tggtaatttt caacaatagc agcaaatgta
    10321 cgtccaactg ctaaaccact accatttaat gtatgtgcta attctggttt agctgctttg
    10381 tcacgcttga agcggatgtt agcacgacgc gcttggaaat ccgtacagtt tgagcatgaa
    10441 ctaatttctt tataatcatt gtagcttggt aaccaaactt ctaaatcata tgttttgctt
    10501 gcactaaatc caatatcacc tgtacataaa ataacacgac ggtatggtaa acctaactct
    10561 tctagaattg cttctgcgtt tgttgtcatt tcttctaaag cattccatga atcttcaggt
    10621 tgttcaaaac gtaccatttc cactttatcg aattgatgta aacgaattaa tcctcttgta
    10681 tctctacctg ctgatcctgc ttcactacgg aaacatgcag attgaccagt gaatttttca
    10741 ggaagtacac ctggttgaat aatttcatta cggtagaaat tcgttaatgg tacttcagca
    10801 gttggaattg tatataatcc ttctttttct actttaaata aatcttcttc aaatttaggt
    10861 aattgacctg taccatacat tgtatctgcg ttcacaagct gtggtaccat catttctgta
    10921 taaccatgtt gtgttgtatg ttttgtaatc atatagttca ttaaagcacg ctctaattgc
    10981 gcaccttcat ttgttaaata tacaaaacgc gcacctgaaa cttttgctgc acgatcaaaa
    11041 tcagccattt tcaattcttc tacaatatcc caatgtgctt tgggttcaaa tgaaaactca
    11101 cgtggtgtac cccacttttt aacttcaacg ttatcttcat cagattcacc ttgaggtaca
    11161 tcatcactta ttaaatttgg aatacgacaa aggatacctg tcattttatt atcaatttca
    11221 tttaattgac tatctttttc tttaatatcg tcacctaatg tgcgcatttc agcaatcaca
    11281 tcatcagcat tttctttatt acgttttttt aatgcgattt cttcgcttac tttattacga
    11341 cgtgctttca tttcttctgt tgcactaatt aatttacgtc gttgctcatc caattctaaa
    11401 atttcatcta caacttttgg atcatctcca cgtaattcaa ttttgctctt aactgtgtca
    11461 ggctcatttc tgaataatct aatgtctaac attaaccttc atcctttccc aaataattat
    11521 catttattat ggaatgacgt acgtctttat tttttagaaa ataaaaaaag accacatccc
    11581 tacaagggac gtggtctacg cgttgccacc ctatttaaca atttaagtta taaagataca
    11641 ctaaacctaa attgcacttc actaaaataa cggttatcac cgattgttct tttaaattaa
    11701 gtaggtagat tcatatatat gttgattctt gttcacacta accacaagct ctctgatatc
    11761 gaacactata tattacttgt cctacgaaca atgtcttatt aagttatttt taatatagca
    11821 aactatattt gctttttcaa gtaacgattt caaacatcac tcatgtcgat ttagtgacat
    11881 gcagtcgttt gataaattga ttgctttaaa tactgtgcaa ccgcttcaat atctttatga
    11941 aattgacgat catgtgtaat ggatggcacg atacttcgaa actcatcata cttgcgacgt
    12001 gtttttggtg ataatccttc aacacctttt aactctgctg cttgtaatgc aataacacat
    12061 tcgattgcca gcacacgtct tgcattttca ataatttgat aaccatgtct agcagctgta
    12121 gttcccatag atacgtgatc ttcttggttc gcagatgaag tgatagaatc aacactcgct
    12181 ggatgcgcta aagttttatt ttcagaaacg agacttgcag cagcatattg cataatcatc
    12241 gcgccacttt gcaatcctgg ctctggacta agaaatgctg gtaaatcacc atttaattga
    12301 ggatttacta gtcgctctag acgacgttcc gatacgtttg ctaattcact tacacctaat
    12361 ttaagatgat ctaatgcaaa agcaataggt tgtccatgga agttaccacc tgaaataaca
    12421 aacgtttcat ttgcttcctc aaatataagt ggattatcat tagccgcatt catttcaaat
    12481 tctaattgct gtttaacata attgaatact tgaaaactcg cgccatggat ttgtggtata
    12541 caacgcaacg tatatgcatc ttgtacacgt atttctgatt gtcgcgtcgt taatgttgat
    12601 ccttctaacc aatcacgcat acgcgctgcc acattaatct gttcttgaaa attacgaact
    12661 gcgtgcacat catgtcgata tgcatctata atgccattaa gagactgatg cgttaatgca
    12721 gcaatccatt cagattggta acctaaatct tctgcttcta tataactaat gacaccttga
    12781 gctgtcatag cttgcgtacc attaatcaat gctaaacctt ctttagcctg aaggttcaaa
    12841 ggttgtctat ttaattctct taatacatcg tcactatcct tttcttcccc tctgtacaat
    12901 actttccctt caccaattaa tgctaatgct aaatgtgata atggcgctaa atctcctgat
    12961 gcaccgagag agccttgctg tgggattatc ggtataatac gttcatttat aaaaaattgt
    13021 aattgtctca ctaattctaa agtggcacct gaatgacctt ttaataatgt attcaatcgt
    13081 aaaatcatca tgactaatgc tacttctttt gaaaatggct cacctagtcc acaggcatgt
    13141 gagcgtatca gattcacttg taattcatta tattgcgtcg ggtctatacg tacatcacta
    13201 aataacccaa aacctgtagt aataccgtaa accgtttcct cattttcaat aatacgttca
    13261 actaccgctc tacttttttt gacacgttct aacgcatcat caataatttc aatctttgat
    13321 tgttgttgta aaaatgattt aatatcctca attgttagtg tttcaccatc taaatataaa
    13381 gtcatatatg ttaccccctt gtttatatta agtaacccat ccttcttgaa gtatacgttt
    13441 tcatttttat tgaaacaatg gttttacgta catttataac ctattatcag agcactattg
    13501 tagtgcgtta aaggatatta agattgttgt aagcatattt aataatttat ctattgacga
    13561 attgcatata caggtatagt attttctatt gtatttaacg acaaataata atgaattcag
    13621 aaatttataa tacattttgt taaaagttac tatatatttt taaaattgaa taaattcgga
    13681 aaaggctttt acatgggagg ttatatcact atggaaacgt taaattctat taacattcct
    13741 aagcgtaaag aagattcaca taaaggtgat tatggcaaaa ttttattaat tggtggatct
    13801 gctaacttag gtggtgccat tatgttagcg gctcgtgcat gtgtatttag cggtagtggt
    13861 ttaatcactg tagctacaca tccaacaaat cattcagcat tacattctcg ttgcccagaa
    13921 gcgatggtta ttgatattaa tgatacgaaa atgttgacga aaatgattga aatgactgac
    13981 agtatactaa ttggtccagg tcttggcgtt gatttcaaag gaaataatgc cattacattc
    14041 ctactacaaa atatacaacc gcatcaaaat ttaatcgtag acggcgatgc gattacaatc
    14101 tttagtaaac tgaaaccgca attacctaca tgtcgtgtga tctttacacc acacctcaaa
    14161 gaatgggaac gattaagtgg tattcctatt gaggaacaga catatgagcg taatcgtgaa
    14221 gcagttgatc gtttaggtgc aactgttgta cttaaaaaac atggtactga aattttcttt
    14281 aaagatgaag actttaaatt gacaatcggt agcccagcaa tggcgactgg tggtatgggc
    14341 gatacacttg ctggtatgat tacaagcttt gtcggtcaat ttgataactt aaaagaagcg
    14401 gttatgagtg ccacatatac acatagtttt attggcgaaa accttgcaaa agatatgtat
    14461 gtggtgccac catcaagact tatcaatgaa ataccttacg caatgaaaca attagaaagt
    14521 tagtcattac taatcattga atatagtaaa gcattacttt ctagcataaa aataagactc
    14581 ccctacatat agggaagtct tattttttat tattcttcat ctgatgattg ttgtatatct
    14641 tcttcaacac gatccatgaa atcttgtctt acttcaatac gtccatcttc atcattttct
    14701 tctgaatcaa tcacttcagt atgaattgca tttcctggtg tttcatcatt tacaaccgct
    14761 tcacgttgtt gttcagtacc atcttcagat acagttgaag tagattgctc atcttcattc
    14821 gtttcatctt ctgcatcttc ttttacttta gcaaccgttg aaacaaattg atcatcacct
    14881 aagcgaatta agcgaacacc ttgtgctgca cgaccatttt gagaaatatc tgcaacatct
    14941 agtcgaataa tgacacctgc attagtaaca atcattaaat cttcttcacc agttactgta
    15001 gtgatacata caacattacc attacgctca gtaatcgtag ctgttttaat acctttacca
    15061 ccacgatttg ataagcgata gtcattaact ggcgtacgtt taccataacc attttcagta
    15121 actactaata cttcatcaac actgtttgca tgagctacat caagccctac aacttcgtca
    15181 ccttcacgaa gtgtaatacc tttcacaccc gttgctgtac ggcctaaagg acgtaatgtt
    15241 gattcaggga atcgaattaa tgatgcatgt gatgtaccaa tcaagatatc ttcttgacca
    15301 cttgttaagc gaactgcaat taactcatca tcttctctga acgaaatcgc aatcttacca
    15361 tttctattta ttcttgagaa gttacttaat gctgaacgtt taacgacacc acgtttagtt
    15421 gcaaacacta agaagttgtc ttcactttca aggtctttaa cagcaatcat tgtactaatg
    15481 acttcatcat tttcaagttc aatagcattc actacaggaa tacctttaga ctgtcttgat
    15541 aactcaggca cttcgtaacc tttaagtttg tatacacgac ctttgttagt aaagaacaat
    15601 acatggtcat gtgtacttaa agttaccaat tgactgacaa aatcttcttc caatgtattc
    15661 ataccttgaa caccacgacc accacggttt tgagcacgat atgtagatac cggcaaacgt
    15721 ttaatgtagt tattatggct tagtgtaatt actatttgtt cttctggaat taagtcttcg
    15781 tcctctaagt tttcaaatcc acctaattga atttctgtac gacgatcatc accgaaacga
    15841 tctctaattt cagtcaattc atctctaact aactgtaata acacttcttc atcagctaag
    15901 attgcttcta attcactaat ataatttaat aactcattat attcagcttc aattttgtct
    15961 ctctctaaac ctgttagacg tcttaaacgc atgtctaaaa tagcttgagc ttgtttttca
    16021 gaaagtttga agcgttgttg caagctttcc attgcaactt tatctgtatc tgactcacga
    16081 atcgttgaaa taatttcatc gatatggtca agtgcgatac gtaatccttc taaaatgtgg
    16141 gcacgatctt tagctttacg taagttgtat tgcgtacgtc ttctaacaac tgtcttttga
    16201 tgctctaaat aatgtaccaa cgcttctttt aaattaataa gcttcggtct accatttaca
    16261 agtgcaatca tattcacacc aaatgatgtt tgaagaggtg tttgtttgta taagttattt
    16321 aaaatgacac tagcatttgc atccttacgc acatcaataa cgacacgcac accagtacgt
    16381 aaacttgttt catcacgtaa atcagtgata ccgtcaattt tcttgtcacg aacgagctct
    16441 gcaatttttt caatcatacg agccttattc acttggaaag gaatttcagt gacaacaata
    16501 cgttgacgtc cgcctccacg ttcttcaata actgcacgag aacgcatttg aattgaacca
    16561 cgacctgttt catatgcacg tctaatacca ctcttaccta aaataagtcc agcagttggg
    16621 aaatcaggac cttcaatatc ctccattaac tcagcaattg aaatatcagg gttcttactt
    16681 aagctaagta caccattgat taattctgtt aagttatgtg gtggaatatt cgttgccata
    16741 cctaccgcga tacctgatgc accattggct aataagttag ggaatcgagc aggtaagact
    16801 gacggctctc tttcattacc atcatagtta tcgataaaat ctattgtatc tttattaata
    16861 tcacgtaaca gttcaagtgt gattttagtc atacgcgctt cagtataacg cattgctgct
    16921 gcgccatctc catccattga accaaagtta ccttggccat caacaagcgg ataacgataa
    16981 ctgaaatctt gagccatacg taccattgct tcataaatag ataagtcacc atgagggtga
    17041 tatttaccca ttacgtcacc aacgatacgt gctgattttt tatatgattt atccggtgtc
    17101 ataccttgtt catttaatcc atatagtata cgacgatgta ctggttttaa accgtcacga
    17161 acatctggca atgcacgagc aacgataaca ctcatcgcat aatctaaaaa tgattcacgc
    17221 atttcactgg taatatttcg ttcatttatt cttgattgag gtaattcagc catcaagagt
    17281 tcctccttca aaagttcagt tcacagcgct tagaagtcta agtttgcata aactgcatta
    17341 tcttctataa attgtctacg gttttctaca acgtcaccca ttaacatttc aaatgtttgg
    17401 tccgcttcaa tcgcatcttc aagttttact tgtaaaagag cgcggtgctc agggttcatt
    17461 gttgtttccc ataattgatc tgcattcatt tctccaagac ctttgtatcg tgcaatagac
    17521 cattttggtg ttggattcaa ttcagattta agtttatcaa gttccctatc attgtataca
    17581 taatactttt gtttaccttg tgtcagttta tacaacggtg gctgtgcaat atacacatag
    17641 cctgcttcaa ttaacggtct cataaatcga tagaagaatg ttaataacaa tgttctaata
    17701 tgcgctccat ccacatcggc atcagtcata atgacgattt tgtgatatct tgctttcgct
    17761 agatcaaagt cgccaccgat tcctgtacca aatgctgtga tcatttgacg aatttcattg
    17821 ttattcaaaa ttctatctaa tcgtgctttt tcaacattta atatcttacc tcgtaatggt
    17881 aaaatcgcct gcgttctaga gtcacgacca gattttgtag accccccggc agagtcccct
    17941 tcgactaaga aaatctcaca ttcttcagga cttttactag agcaatcggc taatttacct
    18001 ggaaggcttg ctacatctaa cgctgattta cgacgtgtta cttcacgcgc ttttttcgca
    18061 gcaacacgtg cacgtgccgc cataatacct ttttcaacca ctgtacgtgc gacttgtgga
    18121 ttttcatata aaaatcgttc aaagtgctct gagaataatt tatctacaac ttgacgcact
    18181 tcagaattac ctaattttgt cttcgtttga ccttcgaatt gaggatcacc atgtttgata
    18241 gatataattg ctgtcatacc ttcacgtgta tcttcaccag aaagtctatc tttttcttct
    18301 ttcataatct tgctacttaa accataacta tttaagacac gcgttaatgc acgtttgaat
    18361 ccgtcttcat gcgtaccacc ttcatacgta tgaatgttat ttgcgtaagt taaaagattt
    18421 gtggcatatc ctgagttata ttgaatcgca atttctactt caatatcatc tttagattga
    18481 tgaatataaa ttggctcatc atgaataggt tctttatttt cgttcaataa ctcaacgtac
    18541 gatttaatac cgccctcata gtgataggag tcttctctaa cgttttcttc atcacgttca
    18601 tctcttaatg tgatttgaat tcctttgttt aagaaagcaa gctctctaat acgctgctgt
    18661 aatgtttcat agttgtatac agttgtctct gtgaagattt ctccatctgc tttaaaacga
    18721 atgacagtac ctgtcttatc agttgtgcca acttctttta agtcaaattg aggtacacct
    18781 tttttatatg cttgatgata tatagtctca tttctgtgta catatacttc taagtcttgt
    18841 gacaatgcgt ttacaactga tgaaccaaca ccatgtaaac caccagatac tttgtatccg
    18901 ccaccgccaa atttaccacc agcatgtaaa acagttaaaa taacttcgac agctggacgt
    18961 cccatttttt cttgaatatc aactgggata ccacgtccgt tatccgttac tttaatccag
    19021 ttatcttttt caataacaac ttcaatttga tttgcataac cagctaatgc ttcatcgata
    19081 ctattatcga caatttccca cactaaatgg tgcaaacctc tctctgaagt cgatcctata
    19141 tacatacctg gtcttttacg tactgcttct aaaccttcta atacttgtat ttgcccagca
    19201 ccataattat ccgtgttgtt tacatctgac aatgcagtca ccatcgcttt ctgttacttt
    19261 ataatttcac cttgattaat acgatacaat ttagcgttat tcatgatttc atgatcaata
    19321 ccatctacag atgtcgtagt gacaaatgtt tgtactttat gctgaatcgt acttaataaa
    19381 tgcgtttgac gcgaatcatc taattcactg agtacatcgt ctaataataa gatgggatat
    19441 tccccaactt cgatattcat taactcaatt tcagctaatt taatggacaa agccgttgta
    19501 cgttgctgtc cttgagaacc atatgtttga gcatccatgc cattcacatc aaaacttata
    19561 tcatctcgat gtggtccgaa taagctaatg cctcgttctt tttctctttg catattatcg
    19621 ctaagaatag acataatttc ttcaagtcgt gccgcttcat tttgagcata atcaaattta
    19681 agactaggta aataattcag cgacaacgct tctttatcat ttgtgatacc agcatgaatc
    19741 ggtttagcta acgactctag ctcttgaata aaatgtgcac gtttatcagt tactttcatt
    19801 gcatattcag caaactgctg atttaatact tccaacattg ttaagtcctt tttttggcct
    19861 aattgtaact gctttaagta attattcttt tgctttaaaa tacgttggta ttgagctaaa
    19921 tcatttaagt aaacagcaga aatttggccc aactccatat ctataaagcg tcgtcttatt
    19981 tgaggagagc cttttacaat attcaaatct tctggcgcaa atagaaccac attgaggtgt
    20041 ccaatatatt gagttagacg actttgctct aagtgattca ctttgacttg tttacctttt
    20101 ttagttataa acattgttaa tggcatcgtg ccgtgtctat aactaagctc accttctatt
    20161 ttagcataat cagcattaaa acgtatgagt tccttatcat tactcgttct atgacttttt
    20221 gctaaagcta aggtataaat tgattcaagt aaatttgtct ttccttgtgc attttctcca
    20281 atgaggatat tcacgtcagg atgacatttc aacgtaacct catcatagtt acgataattt
    20341 tctaattgga gtgtatttaa cttcattgtt caccttgatg aatgattaag aaagaaccag
    20401 catcttcagg taattctggg atatctatac gatcttgatg ttctaacttt ttaccgcgac
    20461 gtgtttcacg cactccatta attaatactt caacgtcttg caagaaccat tttgcttgac
    20521 caccagattc aataatccct tctgttttta gaaattgacc taaattaatg tctccttcta
    20581 ctacaacttc ttgaaccaaa ataatcactc ca
//
LOCUS       DAKTIO010000029        20518 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.029, whole genome shotgun sequence.
ACCESSION   DAKTIO010000029 DAKTIO010000000
VERSION     DAKTIO010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20518
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.029"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..152
                     /locus_tag="O0293_000814"
     CDS             <1..152
                     /locus_tag="O0293_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279708.1"
                     /translation="TKLGYLIIIILGVTVGILIYGTITIKTRLADEFLGEIPEKLRRR
                     VRFLR"
     gene            149..844
                     /locus_tag="O0293_000815"
     CDS             149..844
                     /locus_tag="O0293_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCY0279709.1"
                     /translation="MRIDKFLANMGVGTRNEVKQLLKKGLVNVNEQVIKSPKTHIEPE
                     NDKITVRGELIEYIENVYIMLNKPKGYISATEDHHSKTVIDLIPEYQHLNIFPVGRLD
                     KDTEGLLLITNDGDFNHELMSPNKHVSKKYEVISANPITEDDIQAFKEGVTLTDGKVK
                     PAILTYIDNQTSHVTIYEGKYHQVKRMFHSIQNEVLHLRRIKIADLELDSNLDSGEYR
                     LLTENDFDKLNYK"
     gene            861..1283
                     /locus_tag="O0293_000816"
     CDS             861..1283
                     /locus_tag="O0293_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCY0279710.1"
                     /translation="MAKGNLFKAILGIGGAVAAVLVTRKDSRDKLKAEYNKYKQDPQS
                     YKDNAKDKATQLGTIANETIKEVKTNPKEYANRLKNNPKAFFKEEKSKFTEYDNKTDE
                     SIEKGKFDDEGGAAPNNNLRIVTEEDLKKNKNALSDKE"
     gene            2033..3442
                     /gene="sapep"
                     /locus_tag="O0293_000817"
     CDS             2033..3442
                     /gene="sapep"
                     /locus_tag="O0293_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+)-dependent dipeptidase Sapep"
                     /protein_id="HCY0279711.1"
                     /translation="MWKEKVQQYEDQIINDLKGLLAIESVRDDAKASEDAPVGPGPRK
                     ALDYMYEIAHRDGFTTHDVDHIAGRIEAGKGNDVLGILCHVDVVPAGDGWDSNPFEPV
                     VTEDAIIARGTLDDKGPTIAAYYAIKILEDMNVDWKKRIHMIIGTDEESDWKCTDRYF
                     KTEEMPTLGFAPDAEFPCIHGEKGITTFDLVQNKLTEDQDEPDYELITFKSGERYNMV
                     PDHAEARVLVKENMTDVIQDFEYFLEQNHLQGDSTVDSGILVLTVEGKAVHGMDPSIG
                     VNAGLYLLKFLASLNLDNNAQAFVAFSNRYLFNSDFGEKMGMKFHTDVMGDVTTNIGV
                     ITYDNENAGLFGINLRYPEGFEFEKAMDRFANEIQQYGFEVKLGKVQPPHYVDKNDPF
                     VQKLVTAYRNQTNDMTEPYTIGGGTYARNLDKGVAFGAMFSDSEDLMHQKNEYITKKQ
                     LFNATSIYLEAIYSLCVEE"
     gene            3446..4294
                     /gene="dat"
                     /locus_tag="O0293_000818"
     CDS             3446..4294
                     /gene="dat"
                     /locus_tag="O0293_000818"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HCY0279712.1"
                     /translation="MEKIFLNGEFVSPSEAKVSYNDRGYVFGDGIYEYIRVYNGKLFT
                     VTEHYERFLRSANEIGLDLNYSVEELIELSRKLVDMNQIETGAIYIQATRGVAERNHS
                     FPTPEVEPAIVAYTKSYDRPYDHLENGVNGVTVEDIRWLRCDIKSLNLLGNVLAKEYA
                     VKYNAVEAIQHRGETVTEGSSSNAYAIKDGVIYTHPINNYILNGITRIVIKKIAEDYN
                     IPFKEETFTVDFLKNADEVIVSSTSAEVTPVIKLDGEPVNDGKVGPITRQLQEGFEKY
                     IESHSI"
     gene            4844..5635
                     /locus_tag="O0293_000819"
     CDS             4844..5635
                     /locus_tag="O0293_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HCY0279713.1"
                     /translation="MEQFYQLGWTLDSAGGASGEAYMAEQDGQKLFLKRNSNPFIAAL
                     SAEGIVPKLVWTKRIETGEVVTAQHWKNGRELSSNEMKQTRVAHLLKKIHNSRPLLSM
                     LKRMEMEPITPEIMLNKINASLSREVLTHHIVRKSLTYLEEHIPSLDSRFFTVVHGDV
                     NHNNWLLSDRDELFLVDWEGAMIADPAIDIGMLLYNYVPQQQWSEWLETYGVQESLNL
                     NKRMKWYTVIQSIGLVQWYEEQKRYKDMNTWLKFLNEVMNSNMFI"
     gene            5650..6294
                     /gene="trmB"
                     /locus_tag="O0293_000820"
     CDS             5650..6294
                     /gene="trmB"
                     /locus_tag="O0293_000820"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCY0279714.1"
                     /translation="MRVRYKPWAEDYLKDHPELVDMDGQHAGKMTEWFDKTQPIHIEI
                     GSGMGQFITTLAAQNPHINYISMEREKSIVYKVLDKVKEMGLTNLKIICNDAIELNEY
                     FKDGEVSRIYLNFSDPWPKNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAY
                     SLESMSQFGMYFTKINLNLHQEDDGSNILTEYEKKFSDKGSRIYRMEAKFHSQK"
     gene            6764..7606
                     /locus_tag="O0293_000821"
     CDS             6764..7606
                     /locus_tag="O0293_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCY0279715.1"
                     /translation="MKIGDISIHYLNGGNTKMDGGAMFGVVPKPLWSKQYNANERNQI
                     NLPTHPILIQTAQYNLIIDAGIGNGKLSEKQLRNFGVDEESHIIADLANYNLTPKDID
                     YVLMTHMHFDHAAGLTDQAGHAIFENAIHVVQQDEWHEFIAPNIRSKSTYWDKNKGDY
                     SNKLILFEKHFEPVLGIKMQHSGGHSFGHTIITIESQGDKAVHMGDIFPTTAHKNPLW
                     VTAYDDYPMQSIREKERMIPYFIQQQYWFLFYHDENYFAVKYSDNGENIDAYILRETL
                     VDNN"
     gene            complement(7731..8042)
                     /locus_tag="O0293_000822"
     CDS             complement(7731..8042)
                     /locus_tag="O0293_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCY0279716.1"
                     /translation="MTKLKYIIPTIIAVAIVIISTISIIQFINRKRYNPVKVLNEVKS
                     YFMNVKGSYIVYEPFVHPETDKYRLVYQGGITTIKNGQNIHYDFYADAYTGEVINIVE
                     R"
     gene            8129..9205
                     /locus_tag="O0293_000823"
     CDS             8129..9205
                     /locus_tag="O0293_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCY0279717.1"
                     /translation="MNINKKVTLQRIQTLTELHGAPGFEEEVKNYMTQQMAPYVDEFI
                     ENRMGGFFGVKKSKNPNAKRVMIAAHMDEIGFMITNITKNGMIQFTNLGGVANDIWQG
                     QRLVIKNRNGDKIIGVVSNIPKHFRTGSEGAPEIKDLTLDIGAQNEDEVRERGIDIGD
                     TIVPHTPFTQLSEHRYSAKAWDNRYGCVLAIEILELLKDIELDVDLYVGANVQEEVGL
                     RGAKASAEMIDPDVAFVVDCSPANDVKGNQPLSGELGKGTLIRIKDGTMILKPVFRDY
                     LLKLVEAHDIEHQYYMSPGGTDGGEIHKANIGIPTAVIGVCARYIHSTDSVFDIRDYF
                     AARSLLSEAICNLDNNQIETLQYK"
     gene            9270..9581
                     /locus_tag="O0293_000824"
     CDS             9270..9581
                     /locus_tag="O0293_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCY0279718.1"
                     /translation="MKQLESEQQFESLKQGATVFEFTAGWCPDCRVIEPDLPELEARY
                     PMFDFVSVDRDKFMDICIENGIMGIPSFLVYKNGELLGSYIGKERKSIEQIDAFLAQY
                     V"
     gene            9681..10538
                     /locus_tag="O0293_000825"
     CDS             9681..10538
                     /locus_tag="O0293_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1444 domain-containing protein"
                     /protein_id="HCY0279719.1"
                     /translation="MNTFQMRDKLKERLSHLDVDFKFNREEETLRIYRTDNNKGITIK
                     LNAIVAKYEDKKEKIVDEIVYYVDEAIAQMADKTLESISSSQIMPVIRATSFDKKTKQ
                     GVPFIYDEHTAETAVYYAVDLGKSYRLIDESMLEDLKLTEQQIREMSLFNVRKLSNSY
                     TTDEVKGNIFYFINSNDGYDASRILNTAFLNEIEAQCQGEMLVAVPHQDVLIIADIRN
                     KTGYDVMAHLTMEFFTKGLVPITSLSFGYKQGHLEPIFILGKNNKQKRDPNVIQRLEA
                     NRRKFNKDK"
     gene            10567..11163
                     /locus_tag="O0293_000826"
     CDS             10567..11163
                     /locus_tag="O0293_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HCY0279720.1"
                     /translation="MNLFYNPKYVGDVAFLQIEPVEGELNYNKKGNVVEITNEGNVVG
                     YNIFEISKDITIEEKGHIKLTDELVNVFQKRISEAGFDYKLNADLSPKFVVGYVETKD
                     KHPDADKLSVLNVNVGNDTLQIVCGAPNVEAGQKVVVAKVGAVMPSGMVIKDAELRGV
                     ASSGMICSMKELNLPNAPEEKGIMVLNDSYEIGQAFFE"
     gene            11184..15008
                     /locus_tag="O0293_000827"
     CDS             11184..15008
                     /locus_tag="O0293_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCY0279721.1"
                     /translation="MSWFDKLFGEDNDSNDDLIHRKKKRRQESQNIDNDHDSLLPQNN
                     DIYSRPRGKFRFPMSVAYENENVEQSADTISDEKEQYHRDYRKQSHDSRSQKRHRRRR
                     NQTTEEQNYSEKRGNSKISQQSIKYKDHSHYHTNKPGTYVSAINGIEKETHKPKTHNM
                     YSNNTNHRAKDSTPDYHKESFKTSEVPSAIFGTMKPKKLENGRIPVSKPSEKVESDKQ
                     KYDKYVAKTQTSQNKQLEQEKQNDSVVKQGTASKSSDENVSSTTKSMPNYSKVDNTIK
                     IENIYASQIVEEIRRERERKVLQKRRFKKALQQKREEHKNEEQDAIQRAIDEMYAKQA
                     ERYVGDSSLNDDSDLTDNSTDASQLHTNGIENETVSNDENKQASIQNEDTNDTHVDES
                     PYNYEEVSLNQVSTTKQLSDDEVTVSNVTSQHQSALQHNVEVNDKDELKNQSRLIADS
                     EEDGATNKEEYSGSQIDDAEFYELNDTEVDEDTTSNIEDNTNRNASEMHVDAPKTQEY
                     AVTESQVNNIDKTVDNEIELAPRHKKDDQTNLSVNSLKTNDVNDNHVVEDSSMNEIEK
                     NNAEITENVQNEAAESEQNVEEKTIENVNPKKQTEKVSTLSKRPFNVVMTPSDKKRMM
                     DRKKHSKVNVPELKPVQSKQAVSERMPASQATPSSRSDSQESNTNAYKTNNMTSNNVE
                     NNQLIGHAETENDYQNAQQYSEQKPSVDSTQTEIFEESQDDNQLENEQVDQSTSSSVS
                     EVSDITEESEETTHPNNTSGQQDNDDQQKDLQSSFSNKNEDTANENRPRTNQQDVATN
                     QAVQTSKPMIRKGPNIKLPSVSLLEEPQVIESDEDWITDKKKELNDALFYFNVPAEVQ
                     DVTEGPSVTRFELSVEKGVKVSRITALQDDIKMALAAKDIRIEAPIPGTSRVGIEVPN
                     QNPTTVNLRSIIESPSFKNAESKLTVAMGYRINNEPLLMDIAKTPHALIAGATGSGKS
                     VCINSILMSLLYKNHPEELRLLLIDPKMVELAPYNGLPHLVAPVITDVKAATQSLKWA
                     VEEMERRYKLFAHYHVRNITAFNKKAPYDERMPKIVIVIDELADLMMMAPQEVEQSIA
                     RIAQKARACGIHMLVATQRPSVNVITGLIKANIPTRIAFMVSSSVDSRTILDSGGAER
                     LLGYGDMLYLGSGMNKPIRVQGTFVSDDEIDDVVDFIKQQREPDYLFEEKELLKKTQT
                     QSQDELFDDVCAFMVNEGHISTSLIQRHFQIGYNRAARIIDQLEQLGYVSSANGSKPR
                     DVYVTEADLNKE"
     gene            15032..16345
                     /locus_tag="O0293_000828"
     CDS             15032..16345
                     /locus_tag="O0293_000828"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCY0279722.1"
                     /translation="MTHYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIENYVFTEVALR
                     NKGIKILPFDANNIKEDMVVIQGNAFASSHEEIVRAHQLKLDVVSYNDFLGQIIDQYT
                     SVAVTGAHGKTSTTGLLSHVMNGDKKTSFLIGDGTGMGLPESDYFAFEACEYRRHFLS
                     YKPDYAIMTNIDFDHPDYFKDINDVFDAFQEMAHNVKKGIIAWGDDEHLRKIEADVPI
                     YYYGFKDSDDIYAQNIQITDKGTAFDVYVDGEFYDHFLSPQYGDHTVLNALAVIAISY
                     LEKLDVTNIKEALETFGGVKRRFNETTIANQVIVDDYAHHPREISATIETARKKYPHK
                     EVVAVFQPHTFSRTQAFLNEFAESLSKADRVFLCEIFGSIRENTGALTIQDLIDKIEG
                     ASLINEDSINVLEQFDNAVILFMGAGDIQKLQNAYLDKLGMKNAF"
     gene            16419..16910
                     /locus_tag="O0293_000829"
     CDS             16419..16910
                     /locus_tag="O0293_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HCY0279723.1"
                     /translation="MDWILPIAGIIAAIAFLILCIGIVAVLNSVKKNLDYVAKTLDGV
                     EGQVQGITRETTDLLHKVNRLTEDIQGKVDRLNSVVDAVKGIGDSVQTLNSSVDRVTN
                     SITHNISQNEDKISQVVQWSNVAMEIADKWQNRHYRRGSANYKANNVATDANHSYTSR
                     VDK"
     gene            16984..18342
                     /locus_tag="O0293_000830"
     CDS             16984..18342
                     /locus_tag="O0293_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279724.1"
                     /translation="MTQQQNDKRTLKNKHTYQNEPLPNRKDFVVSFITGALVGSALGL
                     YFKNKVYQKADDLKVKEQELSQKFEERKTQLEETVAYTKERVEGFLNKSKNEQAALKA
                     QQAAIKEEASANNLSDTSQEAQEIQEAKREAQAEADKSVAVSNKESKAVALKAQQAAI
                     KEEASANNLSDTSQEAQEIQEAKKEAQAETDKSAAVSNEEPKAVALKAQQAAIKEEAS
                     ANNLSDISQEAQEVQEAKKEAQAEKDSDTLTKDASAAKVEVSKPESQAERLANAAKQK
                     QAKLTPGSKESQLTEALFAEKPVAKNDLKEIPQLVTKKNDVSETETVNIDNKDTVKQK
                     EAKFENGVITRKADEKTTNNTAVDKKSGKQSKKTTPSNKRNASKASTNKTSGQKKQHN
                     KKSSQGAKKQSSSSKSTQKNNQTSNKNSKTTNAKSSNASKTPNAKVEKAKSKIEKRTF
                     ND"
     gene            18477..18581
                     /locus_tag="O0293_000831"
     CDS             18477..18581
                     /locus_tag="O0293_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279725.1"
                     /translation="MRSFHVFMLFETSLDNNSDIAHFKLKMEKYSIIK"
     gene            19042..20133
                     /locus_tag="O0293_000832"
     CDS             19042..20133
                     /locus_tag="O0293_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="HCY0279726.1"
                     /translation="MSNKLESYRSEIVSLNHQILDLLSKRGELAQKIGEEKLKQGTRI
                     YDPQREKEMLNDLIDSNKGPFNDNTIKQLFKEIFKASTDLQKSENEKHLYVSRKLKPE
                     DTIVTFDNGGIIGDGNKSFVFGPCSVESFEQVEAVAKNLHAKGEKFIRGGAFKPRTSP
                     YDFQGLGVEGLKILKQIKDKYDLNVVSEIVNPNDFEVADEYLDVFQIGARNMQNFELL
                     KEAGRTKKPILLKRGLSATIEEFVYAAEYIASQGNQNIILCERGIRTYEKATRNTLDI
                     SAVPILKQGTHLPVMVDVTHSTGRKDIMLPTAKAALAVGADGVMAEVHPDPSVALSDA
                     GQQMDLDEFQAFYDELKPLADLYNAKKLK"
BASE COUNT         7825 a         2771 c         3799 g         6123 t
ORIGIN      
        1 ctactaaact tggttactta atcattatta ttttaggtgt tacagtaggt attctaattt
       61 acggtacaat cacaattaaa acgcgtcttg cggacgaatt cttaggtgaa attcctgaaa
      121 aattaagacg tagagttagg tttttacgat gagaatagat aaatttttag caaatatggg
      181 cgtcggtaca cgtaatgagg tcaaacagtt acttaaaaaa ggccttgtaa atgtcaacga
      241 acaagtaatt aaatcaccaa aaacacatat agaaccagaa aacgataaga taacagttcg
      301 tggagaatta atagaataca ttgaaaatgt ctatattatg ttaaataagc caaaaggtta
      361 tatatcagca actgaagatc atcattcaaa aaccgttatt gatttaatac ctgaatacca
      421 acatctaaat atttttccgg ttggtcgtct tgataaagat acagaagggc ttttattgat
      481 aacgaatgat ggtgatttta atcatgaatt aatgagtcca aacaaacatg tttctaaaaa
      541 atatgaagtt atttcagcaa atcctatcac tgaagacgac attcaagctt ttaaagaggg
      601 tgtaacactg actgatggca aggttaagcc tgcaatatta acttatattg ataatcaaac
      661 gtcacacgta acaatttatg aaggaaaata tcatcaagtt aaacgaatgt ttcatagcat
      721 tcaaaatgag gtattacatt taagacgtat caaaattgct gatttagaat tggacagcaa
      781 tttagattca ggagaatatc gtttattaac tgaaaatgat tttgacaaat taaattataa
      841 ataaaaggga gacgattgat atggcaaaag gtaatttatt taaagcgatt ttaggtatag
      901 gtggcgctgt agcagctgta cttgttacac gtaaagatag tcgtgacaag ctgaaagcag
      961 aatataataa atataaacaa gatcctcaaa gctataaaga taatgctaag gataaagcga
     1021 cgcaattagg aacaattgct aatgaaacaa ttaaagaagt aaaaacaaat ccgaaagaat
     1081 atgctaatag attaaaaaat aatccaaaag catttttcaa agaagaaaaa tcaaaattta
     1141 ccgaatatga caataagact gacgaaagta ttgaaaaagg taaatttgat gatgaaggtg
     1201 gcgcagcacc aaataataat ttacgtatcg tcactgaaga agatttaaaa aagaataaaa
     1261 atgcattatc tgataaagaa taagatgtga gtaggacaga aatgataagg gagtgggaca
     1321 gaaatcatat tttagcaaaa tttatttcgt cgtcccaccc cggcaaggtt gactagaatt
     1381 gaaaaaagct tgttacaagc gtattttcat tcagtcaact actgccaata taatattgta
     1441 aactatagga catttattag tgtttcaggt tctttatcgt tagtcatagc taatatgcat
     1501 atagaaagaa gaatacatgc gtgaaactca ttcataagaa atactaatat ctaaagaaaa
     1561 cgtatttctt tatcttttcg aaattctctg tgttggggcc cttaccccaa cttgcacatt
     1621 attgaaagct gactattggc cagcttctgt gttggggccc ttaccccaac ttgcacatta
     1681 ttgaaagcta actattggcc agcttctatg ttggggcccc gccaacttgc attgtctgta
     1741 gaatttcttt tcgaaattct ctatgttggg gccccggggc gcattttcgt tcggttaatt
     1801 actgtcaata ttaaattgtg tgcctagtac attgacttat atctcgagct cttgaatatg
     1861 tgtattttaa atatataaat taaaaaagga acaaagcagc tatttatgat ttgtgtcata
     1921 tcaatagcat cgttttgttc ctttttatta ttttctgaca tcttattgta gtttttatat
     1981 cttttcatgt aaaatagaat ttaacttata gaaaaaagga agtcgatttc gaatgtggaa
     2041 agaaaaagtt caacaatacg aagatcaaat cattaatgac ttaaaaggat tattagcaat
     2101 tgaaagtgtg agagatgatg caaaagcatc agaagacgca ccagttggtc caggtcctcg
     2161 taaggcatta gactacatgt atgaaattgc acatagagat ggatttacaa cacatgatgt
     2221 ggatcatatt gcaggaagaa ttgaggcagg taaaggaaat gacgtattag gtatcttatg
     2281 tcatgttgac gttgttcctg ctggtgatgg atgggatagt aatccgttcg agccggttgt
     2341 aacagaagat gctatcatag ctagaggtac acttgatgac aaaggtccaa caattgctgc
     2401 ttattatgca attaagatat tagaagatat gaatgtggat tggaagaaac gtattcatat
     2461 gattattggt acggatgaag aatctgattg gaaatgtacg gatcgctatt ttaaaacaga
     2521 agaaatgcca acattaggtt ttgcaccaga tgcagaattt ccatgtattc atggtgaaaa
     2581 aggcattaca acatttgatt tagttcaaaa taaacttact gaagatcaag atgaacctga
     2641 ttatgaatta ataactttta aatctggtga acgttacaac atggtacctg atcatgcaga
     2701 agcaagagtg cttgttaaag aaaatatgac agatgttatt caagactttg agtacttttt
     2761 agaacaaaat catttacaag gtgatagtac tgttgatagt ggcattctag ttttaacagt
     2821 tgaaggtaaa gcggttcatg gtatggatcc atctatcggt gtgaatgcgg gtctttactt
     2881 actaaaattc ttagcatcat taaatcttga taataatgca caagcgtttg tagcatttag
     2941 taatcgctac ttatttaatt cagattttgg tgaaaagatg ggaatgaaat tccatacaga
     3001 tgtcatgggt gacgtgacaa ctaacattgg tgttattaca tatgataatg aaaacgcagg
     3061 tcttttcggt atcaacttac gctacccaga aggatttgaa tttgaaaaag ctatggatcg
     3121 ttttgcaaat gagattcaac aatatggctt tgaagtgaaa ttaggtaaag tccaaccacc
     3181 acattatgtt gataaaaatg atccttttgt acaaaagtta gttactgcat atagaaatca
     3241 aacaaatgat atgactgaac cttatactat aggtggcggt acttatgcga gaaacttaga
     3301 caagggtgta gcatttggcg caatgtttag tgattctgaa gatttaatgc atcagaaaaa
     3361 tgaatatatc actaaaaaac agttatttaa cgcaactagt atttacttag aagcaattta
     3421 ttcattatgc gtggaggaat aatatatgga aaaaattttt ttaaatggtg agtttgtaag
     3481 tccaagtgaa gcaaaggttt catacaacga cagaggatac gtatttggcg atggtattta
     3541 tgaatacatt cgagtatata atggtaagtt atttacagta acagaacatt atgaaagatt
     3601 tttacgtagt gccaatgaga ttggtttaga tttaaattat tctgtagaag aattaattga
     3661 actatctcgt aaattagttg atatgaatca aattgaaact ggggcaattt atattcaagc
     3721 aacgcgtggt gtagctgaaa ggaatcatag cttcccgaca cctgaagtag aaccagcaat
     3781 tgttgcttat acaaagagtt atgatcgtcc ttatgatcat ttagaaaatg gtgtgaatgg
     3841 tgttaccgtt gaagatatcc gatggttacg ttgcgacatt aaaagcttga acttattagg
     3901 aaatgtatta gcaaaagaat atgctgtgaa atataatgca gttgaagcaa ttcaacatcg
     3961 aggtgaaact gtaactgaag gatcttcaag taatgcttat gcaattaaag acggtgtgat
     4021 ttatacacat ccgattaaca actatattct taatggtatt acacgaattg taattaaaaa
     4081 aattgccgaa gactataaca tcccatttaa agaagaaacg tttactgtag atttcttgaa
     4141 aaacgcagat gaagttattg tttcaagtac ttcagctgag gttacacctg ttattaaatt
     4201 agatggtgaa ccagttaatg atggtaaagt tggcccaatt acacgtcaac tacaagaagg
     4261 atttgaaaag tatatagagt cacacagtat ttaaaattct ttcatcatat ttttagatta
     4321 aattacttaa atttatcaac tttcgtaagt gaattatgtt ataatttaaa acgaagctta
     4381 aaagtgttgt ttagaaaaca catattagta aaaagtgcca ttttgtttct cattttcagt
     4441 tgaaatataa tgataaaaat tataaaattt tctatgagtt acaaatatga tgtgcatcat
     4501 attttactaa gtacgtaaat gttacagttc ataaatttat gctaaccatg aaaatgggat
     4561 agcaattatt ttgaaaacta gagtttatcg cctacagaga ttcaaaaaca tgtagtttta
     4621 gacacggtaa cattatatgt caagtaagtg actatagaat gtatggtgtc aatgacagac
     4681 attacgattt aaatttagtc gtgtaatgta ggcattggtt gaggatttac aataattatc
     4741 tggacataat atttgtacca gtatggtgtt ataaattaga atatatggct cttgaaaaac
     4801 ctatttctag agccattttc taattgaaag tgaggtggac gagttggagc agttttatca
     4861 attagggtgg acacttgatt cagcaggtgg tgcatctggt gaagcatata tggctgaaca
     4921 agatggacaa aagttgtttt taaaacgaaa ttcaaatcca tttattgcgg cattatcagc
     4981 agaaggtatt gtgcccaaat tagtatggac gaaacgcata gaaacaggcg aggttgttac
     5041 agcacaacat tggaaaaatg ggcgtgaact atcttcaaac gaaatgaagc aaacaagagt
     5101 tgcacattta ttaaagaaga tacacaattc tagaccttta ttaagtatgt taaagcgtat
     5161 ggaaatggaa cctattactc ctgagattat gcttaataaa attaatgcct ctttatcaag
     5221 agaagtttta acacatcata ttgtgagaaa atcattaacc tatttagaag agcatatacc
     5281 gagtttagat tcgcgttttt tcactgttgt acatggtgat gtgaatcata ataattggtt
     5341 attatccgat cgagatgaac tatttttagt tgattgggaa ggtgcaatga ttgcagaccc
     5401 ggcaattgat atcggaatgc tgctttacaa ctatgttcca caacaacaat ggtctgaatg
     5461 gcttgaaaca tatggtgttc aagagagctt gaatttaaat aaacgtatga aatggtatac
     5521 ggtaattcaa tctataggtc ttgttcaatg gtatgaagag caaaaaagat ataaggatat
     5581 gaacacttgg cttaaattcc taaatgaagt tatgaatagt aacatgttta tttaagagga
     5641 gaataaaata tgagagttcg atacaaacca tgggctgaag attatttaaa agatcatcct
     5701 gaattggttg acatggacgg tcaacatgca gggaaaatga ctgaatggtt tgataaaacg
     5761 caaccgatac acattgaaat tggttcgggt atggggcagt ttattacaac attagctgct
     5821 caaaatcctc atattaacta tatttcaatg gaacgtgaga aaagtatcgt ttataaagta
     5881 ttggataaag ttaaagagat gggtttaaca aatttaaaga ttatttgtaa tgacgccatt
     5941 gaattaaatg aatattttaa agacggcgaa gtttcgcgta tttatttgaa cttttcagat
     6001 ccatggccaa aaaatcgtca tgcaaaacga cgtttaacgt atcatacgtt tttagcgttg
     6061 tatcaacaaa tcttaaatga tgaaggagat ttacatttta aaactgataa cagaggacta
     6121 tttgcctaca gtttagaaag tatgtctcaa tttggaatgt attttacaaa aataaatcta
     6181 aatttacatc aagaagacga tggcagtaat attttaacag aatatgaaaa gaaattttcg
     6241 gataaagggt cacgtattta tcgtatggaa gcgaaattcc attcacaaaa atagagcact
     6301 tatgagggta tgtacacata caatcataag tgttttttcg tactataatg gttctataga
     6361 taaaggttct ataataaatc ggactgatgg aaaaagtttt taacttttca tctgtccgat
     6421 tttttgattt tgaatataaa aaaagcgcaa ttatctctat aattaaaagt gactaaacaa
     6481 ataactaaag gagataatgc gcctatgaac aatgttatac taaatacctt tgatttaaaa
     6541 gaataaagct tgcattttac ttgtaaattt taattattaa tttaaaaata caggccgatg
     6601 gttatgatgc atttacatat aatatgattg cattaatctt tataagtagt tagaagtgta
     6661 ttagtgtggg gcgtttcgtt tttgttcgca tttatgaata gtgatatgat gtaataaaca
     6721 catgctaatt gtaataacga atacagaaag aataggtgat gttatgaaaa tcggggatat
     6781 atctattcat tatctaaatg gtggcaatac aaaaatggat ggcggtgcaa tgtttggtgt
     6841 tgttccgaag ccgttgtggt caaagcaata caatgcaaat gaacgaaatc aaatcaattt
     6901 accgacacat ccaattttga ttcaaacggc tcaatataat ttgattatag atgcaggtat
     6961 tggtaatggt aaattatctg aaaagcaatt acgtaatttt ggagtagatg aagaaagtca
     7021 tataattgct gatttggcaa attataattt aacgccaaag gatattgatt atgtgctaat
     7081 gacacatatg cattttgatc atgctgccgg tttgactgat caagcaggac atgcaatttt
     7141 tgaaaatgcg attcatgttg tgcaacaaga tgagtggcat gagtttattg cacctaatat
     7201 aagaagtaaa tcaacttact gggataagaa taaaggcgat tatagtaaca agttgatttt
     7261 attcgaaaaa cattttgaac cggttctggg tatcaagatg caacatagtg gaggtcatag
     7321 ctttggccac acgattatta cgattgaaag tcaaggagat aaagcagttc atatgggtga
     7381 tatattccca actactgcac ataaaaatcc tctatgggta acggcatatg atgattatcc
     7441 tatgcaatcg attcgtgaaa aagaacgcat gataccatat tttattcagc aacaatattg
     7501 gttcttgttt tatcatgatg aaaactactt tgctgtaaaa tacagcgata atggtgaaaa
     7561 catagatgca tatattttac gtgaaacatt agttgataat aactaaaata aagatgtatt
     7621 actaaacaaa ttttcaaaaa taaaaaattg agccacatcc aatcttacta attagggtgt
     7681 ggctcatttt taagttttac gatccaaatc aaatatggat aaaattcgta ttaacgctct
     7741 acaatgttaa tgacttcacc agtatatgca tctgcataaa aatcataatg aatattttga
     7801 ccatttttaa tagttgtaat tccaccttga taaactaaac ggtatttatc agtttcagga
     7861 tgaacgaatg gttcataaac gatatatgag cctttgacat tcataaaata tgattttact
     7921 tcattaagta ctttaacggg attatagcgt ttacgattaa taaattggat gattgaaatg
     7981 gtagaaataa ttacaatggc tactgcaatt attgttggaa ttatatattt cagtttagtc
     8041 atcatttatg ctcccttgat ttcaaattca tatcattagt ttaccatatt gaagatgata
     8101 taataactta taaggagtga gaacttttat gaacataaat aaaaaagtaa cattacaacg
     8161 aattcaaact ttaactgagc ttcatggggc accaggtttt gaagaagaag taaaaaatta
     8221 tatgactcag caaatggcgc cgtacgtaga tgaatttatt gaaaatcgta tgggtggatt
     8281 ttttggtgtg aaaaaatcta aaaatccaaa tgcaaaacgt gtaatgattg cagcacatat
     8341 ggatgaaatc ggatttatga ttacaaatat cactaaaaat ggaatgattc aattcacaaa
     8401 tttaggtggt gttgcaaatg atatttggca aggacaacgc ttagtaatta aaaatagaaa
     8461 tggcgataaa attatcggtg ttgtttctaa tatacctaaa cattttcgta ctggtagtga
     8521 aggtgcaccg gaaattaaag atttaacatt agatataggt gctcaaaatg aagatgaggt
     8581 gcgtgagcgc ggaatagata taggagatac aattgtacct cacacgccat tcacacagtt
     8641 atctgaacat cgatatagtg ctaaagcatg ggataatcgt tatggttgtg tcttggcaat
     8701 tgaaatacta gaattattaa aagatataga attagatgta gacttgtatg ttggcgcaaa
     8761 tgttcaagaa gaggttggat tacgaggtgc gaaagcatct gcagagatga tagacccaga
     8821 cgttgcattt gtagttgatt gttcacctgc caatgacgtt aaaggaaacc aaccattatc
     8881 tggtgaactt ggtaaaggga cgttaattcg cataaaagac ggtacaatga ttttaaagcc
     8941 tgtatttaga gactatttat taaagttagt agaagcacat gacattgaac atcaatacta
     9001 tatgtcacca ggtggaacag atggtggaga aattcataaa gctaatattg gtattccgac
     9061 tgcagttatt ggtgtatgtg cacgatatat tcatagtaca gactcagtat ttgatataag
     9121 agactatttt gcagctagat ctttactttc agaagccatt tgtaatttag ataataatca
     9181 aatagaaaca ttacaatata aataatcggg taataacaac tattatctct aaatagttat
     9241 atataatcat taattaagga gacataaaaa tgaaacaact tgaatcagaa caacaatttg
     9301 aatctttaaa acaaggtgct acagtatttg aattcactgc aggctggtgt ccagattgta
     9361 gagtgataga accagattta ccggaattag aagcgagata tcctatgttt gacttcgtat
     9421 cagtagaccg tgataaattt atggatattt gtattgaaaa tggtattatg ggtattccaa
     9481 gttttctagt atataaaaat ggagaactgc ttggaagtta tattggaaaa gaacgaaaat
     9541 caattgaaca gatagatgca tttttagctc aatacgtgta atttagacta gagaaaaacg
     9601 gggtaatact cgtttttctc tgttactatg tgttgattta ttgtaaacta ttataaggtg
     9661 cgaaattagg agtgttacat atgaatacct ttcaaatgag agataaatta aaggaacgtt
     9721 taagccattt agacgttgat tttaaattta atcgtgaaga agaaactttg cgtatttatc
     9781 gaacagataa taacaaaggt atcacgatta aacttaacgc tatagtcgca aaatatgaag
     9841 ataaaaaaga aaaaattgta gatgaaattg tttattacgt tgatgaagct attgcacaaa
     9901 tggcagataa aacccttgag agtatatcat ctagtcaaat tatgcctgtc attagagcga
     9961 ctagctttga taaaaaaact aaacaaggtg ttccttttat ctatgatgag catactgcag
    10021 aaacagcagt ttattatgca gtcgatttag ggaaatcata tcgtctaatt gacgaaagca
    10081 tgttagagga tttgaagtta actgaacaac aaataagaga aatgtctctg tttaatgtta
    10141 gaaaattgtc aaattcatat acgactgatg aagtaaaagg taatattttt tattttatta
    10201 actcaaatga cgggtatgat gcaagtagga tactaaatac tgcattttta aatgaaattg
    10261 aggcacaatg tcaaggcgaa atgctcgtag cagtgccaca ccaagatgtg ttaattattg
    10321 cagatatacg caataaaaca ggatatgatg tgatggcaca tttaacaatg gaatttttca
    10381 ctaaaggtct agttccaatt acatcattat cctttggata taaacagggt catcttgaac
    10441 cgatatttat tttaggtaaa aataataaac aaaaaagaga tccaaacgtg attcagcgtt
    10501 tagaagcaaa tcgtcgtaaa tttaataaag ataaatagaa ataattggat aaggagtttt
    10561 gtcataatga atttatttta caatcctaaa tatgtaggag atgtcgcatt tttacaaatt
    10621 gaaccagttg aaggtgaatt aaactacaat aaaaaaggta atgttgttga aattactaat
    10681 gaaggtaatg ttgtaggtta taatattttt gaaatttcaa aagatataac aattgaagaa
    10741 aaaggtcata ttaaattaac tgatgaactt gtaaatgtat tccaaaagcg tatttcagaa
    10801 gctggttttg attataaatt aaatgctgat ctatcaccga aatttgtagt tggctacgtt
    10861 gaaactaaag acaaacatcc tgatgcagat aaattaagtg tactaaatgt aaacgttgga
    10921 aatgacacat tacaaattgt atgtggcgcg cctaacgttg aagctggaca gaaagttgtt
    10981 gttgctaaag taggtgcagt gatgcctagc ggtatggtaa ttaaagatgc tgaattacgt
    11041 ggtgttgcct caagcggtat gatttgttca atgaaagaat tgaatttacc taatgcacct
    11101 gaagaaaaag gtattatggt attaaatgac agctatgaaa ttggacaagc attttttgaa
    11161 taattaagga aggtagtgaa aatatgagct ggtttgataa attattcggc gaagataatg
    11221 attcaaatga tgacttgatt catagaaaga aaaaaagacg tcaagaatca caaaatatag
    11281 ataacgatca tgactcatta ctgcctcaaa ataatgatat ttatagtcgt ccgaggggaa
    11341 aattccgttt tcctatgagc gtagcttatg aaaatgaaaa tgttgaacaa tctgcagata
    11401 ctatttcaga tgaaaaagaa caataccatc gagactatcg caaacaaagc cacgattctc
    11461 gttcacaaaa acgacatcgc cgtagaagaa atcaaacaac tgaagaacaa aattatagtg
    11521 aaaaacgtgg gaattctaaa atatcacagc aaagtataaa atataaagat cattcacatt
    11581 accatacgaa taagccaggt acatatgttt ctgcaattaa tggtattgag aaggaaacgc
    11641 acaagccaaa aacacataat atgtattcta ataatacaaa tcatcgtgct aaagattcaa
    11701 ctccagatta tcacaaagaa agtttcaaga cttcagaggt accgtcagct atttttggca
    11761 caatgaaacc taaaaagtta gaaaatggtc gtatccctgt aagtaaacct tcagaaaaag
    11821 ttgagtcaga taaacaaaaa tatgataaat atgtagctaa gacgcaaacg tctcaaaata
    11881 aacaattaga acaagaaaaa caaaatgata gtgttgtcaa acaaggaact gcatctaaat
    11941 catctgatga aaatgtatca tcaacaacaa aatcaatgcc taattattca aaagttgata
    12001 atactatcaa aattgaaaat atttatgctt cacaaattgt tgaagaaatt agacgtgaac
    12061 gagaacgtaa agtgcttcaa aagcgtcgat ttaaaaaagc gttgcaacaa aagcgtgaag
    12121 aacataaaaa cgaagagcaa gatgcaatac aacgtgcaat tgatgaaatg tatgctaaac
    12181 aagcggaacg ctatgttggt gatagttcat taaatgatga tagtgactta acagataata
    12241 gtacagatgc tagtcagctt catacaaatg gcatagagaa tgaaactgta tcaaatgatg
    12301 aaaataaaca agcgtcaata caaaatgaag acactaatga cactcatgta gatgaaagtc
    12361 catacaatta tgaggaagtt agtttgaatc aagtatcgac aacaaaacaa ttgtcagatg
    12421 atgaagttac ggtttcgaat gtaacgtctc aacatcaatc agcactacaa cataacgttg
    12481 aagtaaatga taaagatgaa ctaaaaaatc aatccagatt aattgctgat tcagaagaag
    12541 atggagcaac gaataaagaa gaatattcag gaagtcaaat cgatgatgca gaattttatg
    12601 aattaaatga tacagaagta gatgaggata ctacttcaaa tatcgaagat aataccaata
    12661 gaaacgcgtc tgaaatgcat gtagacgctc ctaaaacgca agagtacgca gtaactgaat
    12721 ctcaagtaaa taatatcgat aaaacggttg ataatgaaat tgaattagca ccgcgtcata
    12781 aaaaagatga ccaaacaaac ttaagtgtca actcattgaa aacgaatgat gtgaatgata
    12841 atcatgttgt ggaagattca agcatgaatg aaatagaaaa gaataacgca gaaattacag
    12901 aaaatgtgca aaacgaagca gctgaaagtg aacaaaatgt cgaagagaaa actattgaaa
    12961 acgtaaatcc aaagaaacag actgaaaagg tttcaacttt aagtaaaaga ccatttaatg
    13021 ttgtcatgac gccatctgat aaaaagcgta tgatggatcg taaaaagcat tcaaaagtca
    13081 atgtgcctga attaaagcct gtacaaagta agcaagctgt gagtgaaaga atgcctgcga
    13141 gtcaagccac accatcatca agatctgatt cacaagagtc aaatacaaat gcatataaaa
    13201 caaataatat gacatcaaac aatgttgaga acaatcaact tattggtcat gcagaaacag
    13261 aaaatgatta tcaaaatgca caacaatatt cagagcagaa accttctgtt gattcaactc
    13321 aaacggaaat atttgaagaa agtcaagatg ataatcaatt ggaaaatgag caagttgatc
    13381 aatcaacttc gtcttcagtt tcagaagtaa gcgacataac tgaagaaagc gaagaaacaa
    13441 cacatccaaa caatactagt ggacaacaag ataatgatga tcaacaaaaa gatttacagt
    13501 catcattttc aaataaaaat gaagatacag ctaatgaaaa tagacctcgg acgaaccaac
    13561 aagatgttgc aacaaatcaa gctgtacaaa catctaagcc gatgattcgt aaaggcccaa
    13621 atattaaatt gccaagtgtt tcattactag aagaaccaca agttattgag tcggacgagg
    13681 actggattac agataaaaag aaagaactga atgacgcatt attttacttt aatgtacctg
    13741 cagaagtaca agatgtaact gaaggtccaa gtgttacaag atttgaatta tcagttgaaa
    13801 aaggtgttaa agtttcaaga attacggcat tacaagatga cattaaaatg gcattggcag
    13861 cgaaagatat tcgtatagaa gcgcctattc caggaactag tcgtgttggt attgaagttc
    13921 cgaaccaaaa tccaacgaca gtcaacttac gttctattat tgaatctcca agttttaaaa
    13981 atgctgaatc taaattaaca gttgcgatgg ggtatagaat taataatgaa ccattactta
    14041 tggatattgc taaaacgcca cacgcactaa ttgcaggtgc aactggatca gggaaatcag
    14101 tttgtatcaa tagtattttg atgtctttac tatataaaaa tcatcctgag gaattaagat
    14161 tattacttat cgatccaaaa atggttgaat tagctcctta taatggtttg ccacatttag
    14221 ttgcaccggt aattacagat gtcaaagcag ctacacagag tttaaaatgg gccgtagaag
    14281 aaatggaacg acgttataag ttatttgcac attaccatgt acgtaatata acagcattta
    14341 acaaaaaagc accatatgat gaaagaatgc caaaaattgt cattgtaatt gatgagttgg
    14401 ctgatttaat gatgatggct ccgcaagaag ttgaacagtc tattgctaga attgctcaaa
    14461 aagcgagagc atgtggtatt catatgttag tagctacgca aagaccatct gtcaatgtaa
    14521 ttacaggttt aattaaagcc aacataccaa caagaattgc atttatggta tcatcaagtg
    14581 tagattcgag aacgatatta gacagtggtg gagcagaacg cttgttagga tatggcgata
    14641 tgttatatct tggtagcggt atgaataaac cgattagagt tcaaggtaca tttgtttctg
    14701 atgacgaaat tgatgatgtt gttgatttta tcaaacaaca aagagaaccg gactatctat
    14761 ttgaagaaaa agaattgttg aaaaaaacac aaacacaatc acaagatgaa ttatttgatg
    14821 atgtttgtgc atttatggtt aatgaaggac atatttcaac atcattaatc caaagacatt
    14881 tccaaattgg ctataataga gcagcaagaa ttatcgatca attagagcaa ctcggttatg
    14941 tttcgagtgc taatggttca aaaccaaggg atgtttatgt tacggaagca gatttaaata
    15001 aagaataatt atgagtaagg agttttatat aatgacacac tatcattttg tcggaattaa
    15061 aggttctggc atgagttcat tagcacaaat catgcatgat ttaggacatg aagttcaagg
    15121 atcggatatt gagaactacg tatttacaga agttgctctt agaaataagg ggataaaaat
    15181 attaccattt gatgctaata acataaaaga agatatggta gttatacaag gtaatgcatt
    15241 cgcgagtagc catgaagaaa tagtacgtgc acatcaattg aaattagatg ttgtaagtta
    15301 taatgatttt ttaggacaga ttattgatca atatacttca gtagctgtaa ctggtgcaca
    15361 tggtaaaact tctacaacag gtttattatc acatgttatg aatggtgata aaaagacttc
    15421 atttttaatt ggtgatggca caggtatggg attgcctgaa agtgattatt tcgcttttga
    15481 ggcatgtgaa tatagacgtc actttttaag ttataaacct gattacgcaa ttatgacaaa
    15541 tattgatttc gatcatcctg attattttaa agatattaat gatgtttttg atgcattcca
    15601 agaaatggca cataatgtta aaaaaggtat tattgcttgg ggtgatgatg aacatctacg
    15661 taaaattgaa gcagatgttc caatttatta ttatggattt aaagattcgg atgacattta
    15721 tgctcaaaat attcaaatta cggataaagg tactgctttt gatgtgtatg tggatggtga
    15781 gttttatgat cacttcctgt ctccacaata tggtgaccat acagttttaa atgcattagc
    15841 tgtaattgcg attagttatt tagagaagct agatgttaca aatattaaag aagcattaga
    15901 aacgtttggt ggtgttaaac gtcgtttcaa tgaaactaca attgcaaatc aagttattgt
    15961 agatgattat gcacaccatc caagagaaat tagtgctaca attgaaacag cacgaaagaa
    16021 atatccacat aaagaagttg ttgcagtatt tcaaccacac actttctcta gaacacaggc
    16081 atttttaaat gaatttgcag aaagtttaag taaagcagat cgtgtattct tatgtgaaat
    16141 ttttggatca attagagaaa atactggcgc attaacgata caagatttaa ttgataaaat
    16201 tgaaggtgca tcgttaatta atgaagattc tattaatgta ttagaacaat ttgataatgc
    16261 tgttatttta tttatgggtg caggtgatat tcaaaaatta caaaatgcat atttagataa
    16321 attaggcatg aaaaatgcgt tttaatatgt ttataataga gtagtatggg tatttattat
    16381 taatgacatt attacatgtt aattaggagg cgtttttaat ggattggatt ttaccaattg
    16441 ctggaattat cgctgcgatt gcattcttaa ttttatgtat cggtatcgta gctgtattaa
    16501 attctgttaa gaaaaactta gattatgttg caaaaacact tgacggtgta gaaggtcaag
    16561 ttcaaggtat tactcgtgaa acaacagatt tacttcataa agtaaaccgt ttaactgagg
    16621 atatccaagg taaagtagat cgtttaaact cagttgtaga tgctgttaaa ggtatcggtg
    16681 actcagtaca aacgttaaac agctctgtag atcgtgtaac aaattcaatt acacataata
    16741 tttctcaaaa tgaagataaa atctcacaag ttgttcaatg gtcaaatgtt gcaatggaaa
    16801 ttgcagacaa atggcaaaat agacactacc gtcgtggaag tgcaaattac aaagctaata
    16861 atgtagcaac tgatgcaaat catagctata cttctagagt agataaataa ttaataatta
    16921 aacaaatgca cttaaatgag gttgttactt atttaagtgc atttgttata ggaggatata
    16981 caaatgacgc agcaacaaaa tgataaaaga acattaaaaa ataaacacac ttatcaaaat
    17041 gaaccactac caaaccgtaa agattttgtt gttagtttta taactggcgc gcttgttggt
    17101 tcagctttag gtttgtattt taaaaataaa gtttatcaaa aagcagatga tttaaaagtc
    17161 aaagaacaag aactgtcgca aaagtttgaa gaaagaaaaa cgcaacttga agaaacggtt
    17221 gcctatacaa aagaacgtgt tgaaggattt ttaaacaaat ctaaaaatga gcaagcggca
    17281 ttaaaggcac aacaagcagc gataaaagaa gaagcaagtg caaataattt aagtgataca
    17341 tcacaagaag cacaagagat tcaagaagct aaaagagaag cacaagcaga agcggataaa
    17401 agtgtggctg tatcaaataa agaatcaaaa gcagtggcat tgaaagcaca acaagcagcg
    17461 ataaaagaag aagcaagtgc aaataatttg agtgatacat cacaagaggc acaagagatt
    17521 caagaagcta aaaaagaagc acaagcagaa acagataaaa gtgcagctgt atcaaatgaa
    17581 gaaccaaaag cagtggcatt gaaagcacaa caagcagcga taaaagaaga agcaagtgca
    17641 aataatttaa gtgatatatc acaagaggca caagaggttc aagaagctaa aaaagaagca
    17701 caagcagaga aagacagtga cacattaact aaagatgcaa gtgcagcaaa ggtagaagta
    17761 tcaaaaccag agtcacaagc tgaaagatta gcaaacgctg caaaacagaa gcaagctaaa
    17821 ttaacaccag gttcaaaaga gagtcaatta actgaagcgt tatttgcaga aaaaccagtt
    17881 gctaaaaatg acttgaaaga aattcctcaa ttagttacta aaaagaatga tgtatcagag
    17941 acagagacgg ttaatataga taataaagac actgttaaac aaaaagaagc taaatttgaa
    18001 aatggtgtta ttacacgtaa agctgatgaa aaaacaacta ataatacagc tgttgacaag
    18061 aaatcaggta aacaatctaa aaaaacaaca ccttcaaata aacgaaatgc atcaaaagca
    18121 tctacaaata aaacttcagg tcagaaaaag caacataata agaaatcatc acaaggtgca
    18181 aagaaacaaa gtagttcaag taagtcaact caaaagaata atcaaactag taataagaat
    18241 tcaaaaacaa caaatgctaa atcatccaat gcatcaaaaa cgccaaatgc taaagttgag
    18301 aaagctaaaa gtaaaataga gaaacgtaca ttcaatgact aagattacta aaatcgttga
    18361 ataatcaatc atagcagtaa gctatgtaat atatttttaa aaattgaatt tcaaaataaa
    18421 aggtaggcat tcaggtgctt accttttttg atatcatcag tttattttga aaggcaatgc
    18481 gatcatttca tgtatttatg ttgtttgaaa catcgttaga taacaatagt gatattgcac
    18541 attttaagct gaagatggaa aaatattcga taatcaaata aacaatgaat tttagaaggt
    18601 acaatgacgt tcactaattt aaatatagct gaatgtgttg gtgagtgatg ttcactatag
    18661 atttatatta atatacaaag acaaaggttg ttaattttta ttaagcgtta ggttgaatgt
    18721 atgagaattt tagatttata atagaagata gaaacgaaaa tttttcttaa aagcagtaat
    18781 gttgactcaa aataagctat aataatgaca cttatttaat tgattaacat ttgctaataa
    18841 atatcaatat agaatataac tttccaataa tgactgagaa aatcgaaatg tcagtctcga
    18901 atcatataat tagaaaattg attattttct gtcaatttag ggttgaacta tacatatgat
    18961 attgttagaa tattttttaa cattatattt tattgcttta aagtggaata tacttgaaat
    19021 aattagtaga ggtgagtaag gatgagtaat aaattagaat catacagaag tgagattgta
    19081 tcactgaatc atcaaatttt agacttatta tctaaacgtg gtgaactagc acaaaaaatt
    19141 ggggaagaaa aattaaaaca aggtacacgt atttatgatc cacaacgtga aaaagaaatg
    19201 cttaacgact taatcgatag taacaaagga ccattcaacg ataatactat taagcaatta
    19261 tttaaagaaa ttttcaaagc ctctacagat ttacaaaaat ctgaaaatga aaaacattta
    19321 tatgtatcac gtaagttgaa acctgaagat acgattgtaa catttgataa tgggggcatt
    19381 attggagacg gcaataaatc atttgtattt gggccatgtt cagttgaatc atttgaacaa
    19441 gttgaagctg ttgctaaaaa cttacatgct aaaggtgaaa aatttattcg tggcggtgca
    19501 tttaaaccac gtacatcacc atatgatttc caaggcctag gtgttgaagg acttaaaata
    19561 cttaaacaga ttaaagataa atatgattta aatgttgtca gcgaaatcgt aaatccaaat
    19621 gattttgaag tggctgatga gtatttagac gtattccaaa ttggtgcacg taatatgcaa
    19681 aacttcgagt tattaaaaga agctggccgt acgaaaaagc ctattctatt aaaacgtggt
    19741 ttatctgcta caatcgaaga gtttgtttat gcagctgaat acattgcttc acaaggtaat
    19801 caaaacatta ttttatgtga acgtggaatc cgaacttatg aaaaggcgac acgtaacact
    19861 ttagatattt cagcagtacc aattttaaaa caaggtacac acttaccagt catggtagat
    19921 gttacgcata gtacaggtcg taaagatatc atgttaccaa ctgcgaaagc agcattagca
    19981 gttggtgctg atggagttat ggctgaggtg catccagatc catctgttgc acttagtgat
    20041 gcgggtcaac aaatggattt agatgaattc caagcatttt atgatgaatt aaagccttta
    20101 gctgatttat ataacgctaa aaagttaaaa taatattcca aggaaactat agactactta
    20161 actaatatgt catgttgaag tagaatatta tctttgaatc gacaatttta aacttacagc
    20221 cattctaaga gtatattact tttagagtgg ctattatttt ttgtatagaa ataaaggtat
    20281 actgcactta acgattgtta taatacttcg acacttgttc aatttcacaa ttattaaaga
    20341 ttatgactga tagcagtaat taaaattata actatgaatt atctgtaaaa tataatagat
    20401 tcacacattt gttgctgaaa tgtgaacatt tttcaacaaa tgcaattgat atttgaaaag
    20461 gctttctcaa aacattacaa ttaaaaatga aaaaagttta tataaaatta aaatatat
//
LOCUS       DAKTIO010000030        19999 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.030, whole genome shotgun sequence.
ACCESSION   DAKTIO010000030 DAKTIO010000000
VERSION     DAKTIO010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19999
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.030"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            24..623
                     /locus_tag="O0293_000833"
     CDS             24..623
                     /locus_tag="O0293_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family protein"
                     /protein_id="HCY0279727.1"
                     /translation="MSEKQSNEQVEAFLNKESQWQEEYKYLRALIFNETELEEAYKWM
                     HPCYTLNNKNVVLIHGFKNYVALLFHKGAILEDKYHTLIQQTEKVQAARQLRFENLTE
                     IQARTEEIKYYLAEAIKAEKAGKKVEMKKTEEYVIPKELEAKFEEMPQLESSFYKLTP
                     GRQHQYIYHIGQAKRSETRQKRVEKYINQILEGKGMHDK"
     gene            627..767
                     /locus_tag="O0293_000834"
     CDS             627..767
                     /locus_tag="O0293_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279728.1"
                     /translation="MSKAYRLYNNIQDDTKQPMAHAVGCFFRCVCHGQHFDVGIPLQA
                     WE"
     gene            847..966
                     /locus_tag="O0293_000835"
     CDS             847..966
                     /locus_tag="O0293_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279729.1"
                     /translation="MGGCPSVTMDACALLQKFDFCNNISHFRHFFAIKQPIER"
     gene            1293..2519
                     /locus_tag="O0293_000836"
     CDS             1293..2519
                     /locus_tag="O0293_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0279730.1"
                     /translation="MLSIKHLTKIYSGNKKAVDDISLDIQSGEFIAFIGTSGSGKTTA
                     LRMINRMIEATEGQIEIDGKDVRSMNPVELRRNIGYVIQQIGLMPHMTIKENIVLVPK
                     LLKWTKEEKDKRAKELIKLVDLPESFLERYPAEISGGQQQRIGVVRALAAEQDIILMD
                     EPFGALDPITRDTLQDLVKTLQRKLGKTFIFVTHDMDEAIKLADKICIMSEGKVVQFD
                     TPDNILRHPANDFVRDFIGQNRLIQDRPNDKTVEGVMIKPITIQAEATLNDAVHIMRQ
                     KRVDAIFVVDSNNHLLGFLDIEDINQGIRGHKSLRDTMQQHIYTVQIDSKLQDSVRTI
                     LKRNVRNVPVVDDQQRLVGLITRANVVDIVYDTIWGDSEDTVQTEHVGEDTASSKVHE
                     QHTTNVKVRDIGDDKS"
     gene            2516..3151
                     /locus_tag="O0293_000837"
     CDS             2516..3151
                     /locus_tag="O0293_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0279731.1"
                     /translation="MIEFLHEHGGQLMSKTLEHFYISIVALLLAIIVAVPIGILLSKT
                     KRTANIVLTVAGVLQTIPTLAVLAIMIPIFGVGKTPAIVALFIYVLLPILNNTVLGVQ
                     NIDSNIKEAGKSMGMTQFQLMKDVELPLALPLIIGGIRLSSVYVISWATLASYVGAGG
                     LGDFIFNGLNLYDPLMIVTATVLVTALALGVDALLALVEKWVVPKGLKVSG"
     gene            3168..4109
                     /locus_tag="O0293_000838"
     CDS             3168..4109
                     /locus_tag="O0293_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HCY0279732.1"
                     /translation="MKKIKYILVVFVLSLTVLSGCSLPGLGSKSTKNDVKITALSTSE
                     SQIISHMLRLLIEHDTHGKIKPTLVNNLGSSTIQHNALINGDANISGVRYNGTDLTGA
                     LKEAPIKNPKKAMIATQQGFKKKFDQTFFDSYGFANTYAFMVTKETAKKYHLETVSDL
                     AKHSKDLRLGMDSSWMNRKGDGYEGFKKEYGFDFGTVRPMQIGLVYDALNSEKLDVAL
                     GYSTDGRIAAYDLKVLKDDKQFFPPYAASAVATNELLRQHPELKTTINKLTGKISTSE
                     MQRLNYEADGKGKEPAVVAEEFLKKHHYFDKQKGGHK"
     gene            4109..4804
                     /locus_tag="O0293_000839"
     CDS             4109..4804
                     /locus_tag="O0293_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0279733.1"
                     /translation="MEGNLLQQLFNYYVTNFGYLWDLFFKHLLMSVYGVLFAALIGIP
                     LGILLARYTKLSGFVITIANIIQTVPVIAMLAILMLVMGLGSETVVLTVFLYALLPII
                     KNTYTGIASVDANIKDAGKGMGMTRNQVLRMIELPLSVSVIIGGIRIALVVAIGVVAV
                     GSFIGAPTLGDIVIRGTNATDGTTFILAGAIPIAIIAIVIDVLLRFLEKRLDPTTRHR
                     KNQSNHRPQSINM"
     gene            5098..6498
                     /locus_tag="O0293_000840"
     CDS             5098..6498
                     /locus_tag="O0293_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0279734.1"
                     /translation="MDTSKQFRGDNRLLLGIVLGVITFWLFAQSLVNLVVPLQSTYSS
                     DVGTINIAVSLSALFAGLFIVGAGDVADKFGRVKITYVGLILNVVGSLLIIITPLPAF
                     LIIGRIIQGLSAACIMPSTLAIINEYYIGTRRQRALSYWSIGSWGGSGICTLFGGLMA
                     TYIGWRSIFVVSILLTLLAMYLIKHAPETKAEPIKGMKAEAKKFDVIGLVILVVTMLS
                     LNVIITQTSHFGLVSPLILGLIVVFICSLVGFVYYENKIKHPLVDFSIFKNRGYSGAT
                     ISNFLLNGVAGGALIVINTYYQQQLGFNSSQTGYISLTYLITVLSMIRVGEKILSQHG
                     PKRPLLLGSGFTVIGLILLSLTFLPEVWYIISSIVGYLLFGTGLGLYATPSTDTAVAS
                     APDDKSGVASGVYKMASSLGNAFGVAVSGTVYTVLAANLNLNLGGFTGMMFNALLAIV
                     AFLVILLLVPKNQTNL"
     gene            6724..7659
                     /locus_tag="O0293_000841"
     CDS             6724..7659
                     /locus_tag="O0293_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCY0279735.1"
                     /translation="MKIAIAGSGALGSGFGAKLFQAGYDVTLIDGYTSHVEAVKQHGL
                     NITINGEAFELNIPMYHFNDQPDESIYDVVFLFPKSMQLKEVMEDMKPHIDNETIVVC
                     TMNGLKHEEVIAQYVAQSQIVRGVTTWTAGLESPGHSHLLGSGPVEIGELVDEGKENV
                     IKVADLLNEAELNGVISKDLYQSIWKKICVNGTANALSTVLECNMASLNESSYAKCLI
                     YKLTQEIVHVATIDNVHLNVDEVFEYLVDLNEKVGAHYPSMYQDLIVNNRKTEIDYIN
                     GAVATLGKQRHIEAPVNRFITDLIHTKESQRHAQD"
     gene            7981..8832
                     /locus_tag="O0293_000842"
     CDS             7981..8832
                     /locus_tag="O0293_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCY0279736.1"
                     /translation="MSKIFVTGATGLIGIKLVQRLKEEGHEVAGFTTSENGQQKLAAV
                     NVKAYIGDILKADTIDQALADFKPEIIINQITDLKNVDMAANTKVRIEGSKNLIDAAK
                     KHDVKKVIAQSIAFMYEPGEGLANEETSLDFNSTGDRKVTVDGVVGLEEETARMDEYV
                     VLRFGWLYGPGTWYGKDGMIYNQFMDGQVTLSDGVTSFVHLDDAVETSIQAIHFENGI
                     YNVADDAPVKGSEFAEWYKEQLGVEPNIDIQPAQPFERGVSNEKFKAQGGTLIYQTWK
                     DGMNPIK"
     gene            complement(8924..10420)
                     /locus_tag="O0293_000843"
     CDS             complement(8924..10420)
                     /locus_tag="O0293_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCY0279737.1"
                     /translation="MTTKKLYFLSISIIILVAISIAIYITLNSNTKTRLTNDSQQQID
                     TIIEHDLQKGHIPGASILIVKNGKVFLNKGYGYQDVDKKVKASPTTKYEIASNTKAFT
                     GLAILKLAQEGRLNLNDAVSKHVPHFKMNYNGQNETITIKQLLAQTSGIPSDITSEDS
                     VTSKNNRLNDVTRAIMGDELHHKPGEEFEYSNMNYDLLGLIIQNVTKQSYTKYITNSW
                     LKPLHMTHTSFKQTNYKSKHDAIGYELQGSTPVVSKPEFNLWDTPSAYMMTSTEDLEH
                     WIKFQLNPPDKYKSLVQQSHKNLSSTIGEPNANAYASGWFTNNDEHLVFHSGTLDNFS
                     SFILLNPKQNYGIVVLANLNSEYVPKLVEHLNTQIVNHKRYSTVASMLNQYKDQFNIV
                     TVLMTTLILLAFIFSAYRAWQMRHGQILLRRSKRIAVLSWLSLCICIALALILYALPY
                     LILGSNNWSFVLTWLPIEIKLALITTLIALFSTLIVILLFLHTKITKT"
     gene            10652..12481
                     /locus_tag="O0293_000844"
     CDS             10652..12481
                     /locus_tag="O0293_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCY0279738.1"
                     /translation="MFNQFKRLIIGQPKKNRELKDEKISKFKGLAILSSDALSSVAYG
                     PEQILITLSVVGAVATWYTLPIAGAVLILLAALIMSYRQIIYAYPKGGGAYMVSKTNL
                     GEKWGLLAGGSLLVDYILTVAVSISSGADAFVAAFPSLYGHKVLIACLLVLFILILNL
                     RGLTESATVLSYPVYLFIIGLVILIFIGTFRVATGDIQPHMHASVGTAVPGVTLFLLL
                     KAFSSGASSLTGVEAISNAVTNFREPSAKNAVKTLIAMGSILAFLLVGIVGLAYVYGI
                     LPQTETTVLSQLAMQIFGDNAAFYFVQATTVMILVLAANTGFTAFPMLAASMSKDKYM
                     PRMFTVRGDRLGYSNSIIILGVLAIILIIVFDGMTEDLIPLYAVGVFIPFTLAQFGMV
                     IKWIHERPKNWLSKLSVNLLGGIVTFIVFMILLITKFSQVWPILIFLPFVVIFFLKIN
                     KHYRDIAEQLRSDIDVLNVDVVDRNLAIVPITSITTAVDKSIYYAQMLANNDVIAVHV
                     SFGDEDEKAFQEKWKRHFPDVRLVILHSEYRSIIRPISRFIDKINRKANDQNYMITVV
                     IPEFITKKRWHNLLHNQTSLRMKLYLIYQKNVNVCTIPFKLKK"
     gene            complement(12621..14699)
                     /locus_tag="O0293_000845"
     CDS             complement(12621..14699)
                     /locus_tag="O0293_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCY0279739.1"
                     /translation="MALLEAFLIFIFAVIISSVINNRFPQIPTAFIQIALGVVIFIIP
                     IQVDFQFNSEVFMFAVIAPLLFVEGTHVSRTKLLEYRKPILLMSMALVFATVVGVGYF
                     IHWIWPALPMPAAFAIAAILCPTDAVAVSAITRGKLLPKGSMTILEGESLLNDAAGII
                     SFKIAVTALVTGTFSLFQAVEQFIISTILGVLIGAIIGFVVVRIRIDLTANKGLKDNN
                     TLTFIQLLTPFVVYFLAEEVHASGIIAVVIAGLIHGLERDRLIRAQTELQMNYHQIWN
                     TFSYALNGFVFVVLGFMIPTVVIDIFQTEPDNFSFLIVITILIAIAIYACRFVWVYFW
                     YKDFYFPKNIQSYLDEEHDSHETPPSRVRYAFIMTMCGIHGTISLSMALTLPFIITKG
                     QAFEYRNDLLFIASFMVLISLILAQIVLPLITPSAEDTTFKGMTYQSAKIFIVQKVIQ
                     HFKNESKKDKNDTNYRPVLNQYYGELLFLLNSEPDNQNTKELKRLEDIAKVIETSTLE
                     RLIDKGKATYQDINNYRNIVELTETHRTASFVEKFANFFKMLWLRLKAMKHYKALNKE
                     SKKQEFENSFKDVQKIMRIVNHNIILRLKEEQNSTNVLEVSLVINHYYDMSRSLKWRA
                     QRRKERQENSNQIIPQAMFHNHKLEALYLQRHLLDELIRKNKINNIVAAQIRENINYN
                     EIVLSLQSKH"
     gene            complement(14837..16246)
                     /locus_tag="O0293_000846"
     CDS             complement(14837..16246)
                     /locus_tag="O0293_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0279740.1"
                     /translation="MARKLHRELNNRHIQLIAIGGAIGTGLFLGSGQTISLTGPSLLF
                     TYMIIGVVLFAFMRALGELLLSNTRFNSFVDIANEYLGPFGGFVIGWTYWLCWIVSSM
                     SDLTAMGQYFAFWYPQVPNWITVLFIVLILISFNLLGARLFGELEFWFSIIKVVTIIA
                     MVIVGLVLIFFSFKTHYGHASFTNLISHGGMFPGGTFGFLMSFQIAVYSFIGIELIGV
                     TAGETKDPEKTLPKAINNVPIRILLFYIGGLLVIMSVIPWNDIDPNSSPFVKLFTLIG
                     VPFAAGVVNFVVLTAAASATNSGIYSNSRILFGLSQQGLGPKVLNKTNSHGVPYLSML
                     VSSIALLIAALLNYIFPNAIQLFIYVTTLSTVLFLVVWAMIIVAYLMYLKKHPEAHKN
                     SKFKLIGGKPIAYIILAFFFFVFILLFFSDETRAAIYISPFWFIFLFFFYKKYKTNAE
                     KLAYEQRQNDSGHFRYDNQ"
     gene            16532..17338
                     /locus_tag="O0293_000847"
     CDS             16532..17338
                     /locus_tag="O0293_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCY0279741.1"
                     /translation="MKIGTIADLHIDRHNKKTSEDYLEALVEIVKYKKLDILLIAGDI
                     SNHYQLTHQFITQLTKQLDIPVKFVPGNHDLWEVESMTTQDIWNNYKSMSQCLVGKPF
                     IVNEEWAIIGHTGWYDYSFAAQRFSLDELQKGKHYGATWQDKERISWGISDQNLSKIA
                     AEQVKKDILEVGNRRVILVTHVVTHPDFIVPMPHRIFDFYNAFIGTSDFNPLYAMFDI
                     PYSIMGHVHFRKSVIDDGRCYLCPCLGYPRQWRSEDIYQEINETIQIIEI"
     gene            complement(17401..18057)
                     /locus_tag="O0293_000848"
     CDS             complement(17401..18057)
                     /locus_tag="O0293_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCY0279742.1"
                     /translation="MNRKILPVKPISQLFPIPMVMGCEGVSAAMMLQYNNQHIPATEI
                     MRHWPTHPNNPHKGYVGHHLFIKLGNYHQTIFPEAYVPFLQKYNPNIVDGTGKSLEDL
                     KGIIDQGQPVLIYHTNLGSKPLLRVFRFDNKPAKQVSNIHVTLLIGYDDYYYYYIDPL
                     WSHIRRGLVLPAIIPNRKQIIKIRKEKMEYSFNSPGRKCIYVQPHSYTIENQQQNKHT
                     "
     gene            18170..18643
                     /locus_tag="O0293_000849"
     CDS             18170..18643
                     /locus_tag="O0293_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCY0279743.1"
                     /translation="MEYQLQQLASLTLVGIKETYENGRQAQQHIAGFWQRCYQEGVIA
                     DLQLKNNGDLAGILGLCIPELDGKMSYMIAVTGDNSADIEKYDVITLASSKYMVFEAQ
                     GAVPKAVQQKMEEVHHYIHQYQADTVKSAPFFELYQDGDTTSEKYITEIWMPVKG"
     gene            complement(18766..19977)
                     /locus_tag="O0293_000850"
     CDS             complement(18766..19977)
                     /locus_tag="O0293_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HCY0279744.1"
                     /translation="MQSTKTKTKHFSFLLLITLGVMTAFGPLTIDMYVPSLPKVQGDF
                     GSTTSEIQLTLSFTMIGLALGQFIFGPLSDAFGRKRIAVSILIIFILVSGLSMFVDQL
                     PLFLTLRFIQGLTGGGVIVIAKASAGDKFSGNALAKFLASLMVVNGIITILAPLAGGL
                     ALSVATWRSIFTILTIVALIILIGVASQLPKTSKDELKQVNFSSVIKDFGSLLKKPAF
                     IIPMLLQGLTYVMLFSYSSASPFITQKLYNMTPQQFSIMFAVNGVGLIIVSQVVALLV
                     EKLHRHILLIILTIIQVVGVALIILTLTFHLPLWVLLIAFFLNVCPVTSIGPLGFTMA
                     MEERTGGSGNASSLLGLFQFILGGAVAPLVGLKGEFNTSPYMIIIFITAILLVSLQII
                     YFKMIKKQHVA"
BASE COUNT         6770 a         2893 c         3833 g         6503 t
ORIGIN      
        1 aaaacaaaat ggaagtgatg actatgagtg agaaacaatc aaatgaacaa gttgaagcat
       61 ttttaaataa agagagtcaa tggcaagaag aatataaata tttgagagcg ttaatcttta
      121 atgaaacaga attagaggaa gcgtataaat ggatgcatcc ttgttacacg ttgaataata
      181 aaaatgtagt acttatccat ggcttcaaaa attatgttgc actattattt cataaaggtg
      241 ccattttgga ggataaatat catacactca ttcaacagac tgaaaaggtg caagcagctc
      301 gtcagttacg atttgaaaat ttaacagaga ttcaagcacg taccgaagaa attaaatatt
      361 atctagccga agcaattaaa gctgaaaaag ctggtaaaaa agttgaaatg aagaaaacag
      421 aggaatatgt tattcctaaa gagttagaag ctaagttcga agaaatgcca cagttagagt
      481 cttcatttta taaattaacg ccaggcagac aacatcaata tatatatcat attggacaag
      541 ctaaacgcag tgaaacaaga caaaagcgtg ttgaaaagta tattaaccaa atactagaag
      601 gtaaagggat gcatgataag taattaatga gtaaagcata ccggttatac aacaacatac
      661 aagatgacac gaaacaacca atggctcatg ctgttggttg tttttttagg tgtgtctgtc
      721 atgggcaaca ctttgacgtt ggaattccgt tacaggcttg ggagtagaaa atgttagcaa
      781 aaggcaaggg tgtctacaat gaatgatgaa gatattaaaa tataaggatg actttgtgag
      841 tggcggatgg gcggttgtcc gtctgtaaca atggatgcgt gtgcattatt acaaaaattc
      901 gacttttgta ataatatttc acattttcga cacttttttg ctataaaaca accaattgag
      961 cgataataaa ttcgctttta aaaaatatga gttatctatt tagttgccaa agataaaata
     1021 ataatgttta ataacatcat atagagtatg ttagttttaa atgtcgaata tacgaatgtg
     1081 caaacaaagt aatcggtaga aattcaacat acatagcgcc gtttactgtt aagtattcac
     1141 attacagatg aaaaatataa aattctacat aatcaagacc atgatgtgta cttgtttaac
     1201 ttatgactct atttgtttaa caattgcgat aatggtcttt ttattttatg cgtatcattc
     1261 gtcatatttt ttatgaggaa ggagaaatga ttatgttaag tattaagcat ttaacgaaaa
     1321 tttattctgg taataaaaag gcagtagatg acatctcttt agatattcaa tctggggaat
     1381 ttatcgcatt tattggaacc agtggaagtg gcaaaacgac tgctttaaga atgataaacc
     1441 gtatgattga agcgacagaa ggacaaattg aaattgatgg taaagatgtt cggagtatga
     1501 atcctgtcga attgcgtaga aatattggct atgttattca acaaattggc ttaatgcctc
     1561 atatgacgat taaagagaat attgtgttgg tacccaaatt gttgaaatgg actaaagagg
     1621 aaaaggataa acgtgcaaag gaattaatta aacttgtgga tttaccggag tcatttttag
     1681 agcgttatcc agcagaaata tcaggtgggc aacaacaacg tatcggtgtt gtaagagcac
     1741 ttgcggccga acaagatatt attttaatgg atgaaccttt tggtgcattg gatcctatta
     1801 cgagagatac gttacaagat ttagttaaaa cgttacaacg aaaattaggc aagacgttta
     1861 tctttgtaac acatgatatg gatgaagcga ttaaattagc agacaaaatt tgtattatgt
     1921 cagaaggtaa ggtggtgcaa tttgatacgc cagacaatat tttaagacat cccgcaaatg
     1981 attttgtacg tgattttata ggacaaaata gactgattca agaccgtccc aatgacaaga
     2041 ctgtagaagg tgtaatgatt aaaccaatca cgatacaagc agaagcaaca ctgaatgacg
     2101 ccgttcatat tatgagacaa aaacgtgttg atgctatttt tgtagtagat agtaataacc
     2161 atttactagg tttcttagac attgaagata taaatcaggg tatacgtgga cacaaaagtt
     2221 tacgagacac catgcaacaa catatttata ccgttcaaat tgattctaaa ttacaagatt
     2281 ctgtacgtac gattttaaaa agaaacgtta ggaatgtacc tgtcgtagat gatcaacagc
     2341 gtttagtagg actgattacg cgtgccaatg ttgttgatat tgtatatgac acgatttggg
     2401 gcgatagtga ggatacagtg caaacagaac atgtggggga agacactgcg tcctcaaaag
     2461 tgcatgagca acacactact aatgtcaaag tacgtgacat aggagatgat aaatcatgat
     2521 tgagttccta catgaacatg gtggacagtt gatgtcgaaa acactggaac atttctatat
     2581 ttctatagtg gcattattac ttgccatcat tgttgcagta cctataggca ttttattatc
     2641 aaaaacaaag cgaactgcca atattgtatt aactgtggca ggtgtcttac aaactattcc
     2701 aacactagct gtacttgcta ttatgatacc gatttttggt gttggtaaaa cgcctgcaat
     2761 tgtagcgcta tttatttatg tattattacc tattttaaat aacacggtac tcggtgttca
     2821 aaatattgat agcaacatta aagaagctgg aaaaagtatg ggaatgacac aatttcaatt
     2881 gatgaaggat gttgaattgc cgttagcatt gccgcttatc attggtggca ttcgtttgtc
     2941 atctgtgtat gtaattagtt gggctacact tgcaagttat gtaggtgcgg gtggattagg
     3001 tgatttcatt ttcaatggtt taaatttata tgatccactg atgattgtaa ctgcaacggt
     3061 actcgttact gcactagcat taggtgttga tgccttatta gctttagttg aaaaatgggt
     3121 agttcccaaa ggcttaaaag tatctggata attaggaggc taagataatg aagaaaatta
     3181 aatatatact tgtcgtgttt gtcttatcgc ttaccgtatt atctggatgt agtttgcccg
     3241 gactaggtag taagagcacg aaaaatgatg tcaaaattac agcattatca acaagcgaat
     3301 cgcaaattat ttcacatatg ttacggttgt taatagagca tgatacacac ggtaagataa
     3361 agccaacatt agtaaataat ttagggtcaa gtacgattca acataatgcc ttaattaatg
     3421 gggatgctaa tatatcaggt gttagatata atggcacaga tttaacggga gctttgaagg
     3481 aagcaccaat taaaaatcct aagaaagcaa tgatagcaac acaacaagga tttaaaaaga
     3541 aatttgatca aacgtttttt gattcgtatg gttttgcgaa tacgtatgca ttcatggtaa
     3601 cgaaggaaac cgctaaaaaa tatcatttag agacagtttc agatttagca aagcatagta
     3661 aagatttacg tttaggtatg gatagttcat ggatgaatcg taaaggcgat ggctatgaag
     3721 gatttaaaaa agagtatggt tttgactttg gtacagtgag accaatgcaa ataggtctag
     3781 tctacgacgc attaaactca gagaagttag acgttgcatt aggttattct acagatggtc
     3841 gaattgcggc gtatgatttg aaagtactta aagatgataa acaatttttc ccaccttatg
     3901 ctgcgagtgc tgttgcaaca aatgaattat tacggcaaca cccagaactt aaaacgacga
     3961 ttaataagtt gacaggaaag atttcgactt cagagatgca acgcttgaat tatgaagcgg
     4021 atggtaaagg taaagaacct gctgtcgtcg cagaagaatt tttgaaaaaa caccactatt
     4081 ttgataaaca gaaaggtggt cataagtaat ggaaggtaat ttattacagc aattattcaa
     4141 ttattatgtt acgaactttg gttatctatg ggatttattt ttcaaacact tattaatgtc
     4201 tgtctatggt gtgctgtttg cagctttaat tggtattcca ttgggaatct tgcttgcaag
     4261 atacacaaaa ctttctggat ttgtaattac aattgcaaat ataattcaaa cagttccagt
     4321 cattgcaatg ttagctattt taatgttagt catgggctta ggttcagaaa cagtagtttt
     4381 aacagtgttt ttatatgcgt tacttccaat tataaaaaac acttatactg gtatagctag
     4441 tgttgatgcg aatattaagg atgctggcaa aggtatggga atgacacgca atcaagtgct
     4501 acgaatgatt gaattaccgt tatctgtttc ggttattatc ggtggcattc gtattgcctt
     4561 ggttgttgcg ataggtgttg ttgccgttgg atcatttata ggagcaccta cgcttggtga
     4621 cattgtgatt cgtggtacaa atgcgacgga tggcacaacg tttattttag caggtgcgat
     4681 tccgattgct atcattgcaa tcgtcattga tgtactatta agatttttag aaaaacgatt
     4741 agacccaaca acacgacatc gtaaaaatca atctaatcat cggccgcaaa gtattaatat
     4801 gtaatagtag aagatgttta taatttagcg atttcgtttc atgatttata aaaaatgagg
     4861 ctactcaagg agctcaaata atctttgagt agccttttta taggttgtgt ttgtatgcgt
     4921 ttacactaaa atagcaatta ttatcatgaa agtttttgga taaaaagcgt taattattgt
     4981 aaaaatacta aaaaatgaga tgttttattt ataattttct gcaaatttat gatattgttt
     5041 cttaatatat catattaaaa atttgttttt cttaaacata ggaggcttat ctaattcatg
     5101 gacacatcaa aacaatttag aggtgacaac cgattgcttt tgggtatcgt tttaggggtt
     5161 attacctttt ggctattcgc gcagtcactt gttaatcttg ttgtcccatt acaatcaaca
     5221 tatagtagtg acgttggaac gataaatatc gctgttagct tatctgcctt atttgctggt
     5281 ttgtttatcg taggtgctgg tgatgttgct gataaatttg gtcgcgtcaa aattacttat
     5341 gtaggattga tattaaatgt tgtaggttca ttactcatca tcattacacc tttgccagca
     5401 tttttaatta taggtagaat aattcaaggt ttgtctgcag catgtattat gccatcaaca
     5461 cttgctatta ttaacgaata ttatattggt acaagaagac aacgtgcctt aagctattgg
     5521 tctattggtt cttggggtgg tagtggtatt tgtacgttgt ttggtggctt aatggctaca
     5581 tatataggtt ggcgttcaat atttgttgtt tcaattctat taacattatt agcaatgtac
     5641 ttaatcaaac atgcacctga gactaaagca gaaccaatca aaggtatgaa agcagaagct
     5701 aaaaagtttg acgttattgg tttagtcatt ttagtagtga cgatgttaag tttaaatgta
     5761 atcatcacac agacgtctca ttttggttta gtttcaccgt taattctagg tttaattgtt
     5821 gtgtttatct gttcattagt tggttttgta tattacgaaa ataaaataaa gcatccactt
     5881 gttgattttt caatttttaa aaatagagga tacagtggtg caactatttc aaacttctta
     5941 ttaaatggtg tagcaggtgg tgcacttatc gttattaaca cgtattatca acaacaatta
     6001 ggatttaatt cttcgcaaac gggttatatt tcattaacgt atttaataac agtgttgtca
     6061 atgattcgtg taggtgaaaa gattttatct caacatggtc cgaagcgccc actattacta
     6121 ggaagtggct ttacagtgat tgggttaatc ttattgtcgt taacattttt accagaagtg
     6181 tggtatatca tatctagtat agttggatat ttattgtttg gtactggttt aggattatat
     6241 gctacaccat caactgatac agcagttgct agtgcgccag atgataagtc gggtgttgct
     6301 tcaggtgtgt ataaaatggc gtcatcatta ggaaatgcat ttggagtagc agtatctggt
     6361 acggtttata ctgtgttagc agctaattta aatttgaact taggtggttt cacaggtatg
     6421 atgtttaatg ccttgctagc aattgttgca tttttagtca ttttactatt agttcctaaa
     6481 aatcaaacga atttgtaaaa ctgaaatgaa agcaagttat tatgtaggga ttttaaagga
     6541 aattttgtga aagtaagttt atcatacaca cttaatgttg cgtattgacg tttaatgtta
     6601 ggtgtgttct tttatagacg ataaaagctg tgtgcatatt aagcgaatga ttttcaaatt
     6661 gacgctaata tgcgaaagta gtatttttaa aatgaacaac aacgatgaag aggggtttat
     6721 aggatgaaaa ttgcaattgc tggatcgggt gcattaggta gtggctttgg tgccaaacta
     6781 tttcaagcag gatatgatgt cacacttatt gacggatata catctcatgt tgaagcggtt
     6841 aagcaacatg gattaaatat aacgattaat ggagaggcat tcgagttaaa cattccgatg
     6901 tatcatttta atgatcaacc ggacgaaagc atttacgatg ttgtctttct atttccaaag
     6961 tctatgcaat taaaagaagt gatggaagat atgaagccac atattgataa tgaaacgatc
     7021 gtcgtatgta cgatgaatgg tctgaagcat gaagaagtca ttgcgcagta tgttgctcaa
     7081 tcacaaattg tcagaggtgt tacgacttgg acggcaggtc ttgaaagccc tggacacagt
     7141 catttacttg gtagtggacc agttgaaata ggtgaactag tggatgaagg taaagaaaat
     7201 gttataaaag ttgctgattt acttaacgaa gcggaattga atggtgtcat tagtaaagat
     7261 ttataccaat cgatttggaa aaagatttgt gttaatggta cggcaaatgc attaagcaca
     7321 gtgttggagt gtaatatggc atcgctgaat gaaagtagtt atgcgaagtg tttgatttat
     7381 aaattaacgc aagaaatagt gcatgtagcg acgattgata atgttcattt aaatgttgat
     7441 gaagtatttg aatatttagt tgatttaaat gaaaaagttg gtgcgcatta tccatccatg
     7501 tatcaagatt taattgttaa taatagaaaa actgaaattg attatattaa tggcgcagtt
     7561 gcaacattag gtaaacaacg tcatattgaa gcgccagtca atcgctttat tactgattta
     7621 attcatacta aagaaagtca gcgacacgca caggattaat tggtgtttta ggtatgatta
     7681 caaggaaatg acaatcacgt gatattacgg tcattattaa gattgaaatg taataaataa
     7741 agaacagcag taaggtactt tcaaattgaa atgatcttgg tgctgttttt cttgattgat
     7801 cttcgtcata attcagattt gtcataggtc tacgacatac tattagtatt tactagacag
     7861 tttttacgac gacactttga aaaattttga ggcaaatcat ttggaagtct cacgtgaatt
     7921 ttgtaaactc atcaagcaag taattatatt aaaaagacaa atagagaaaa ggtgtttata
     7981 atgagtaaaa tttttgtaac tggtgcaacg ggccttattg gcattaaatt agttcaaaga
     8041 ctaaaagaag aggggcatga ggttgctggt tttactacat ctgagaatgg tcaacaaaag
     8101 ctagctgctg ttaatgtaaa agcatatatt ggtgatatat taaaagctga tactattgat
     8161 caagcgttag cagattttaa accagaaatc attatcaatc aaattacgga tttaaaaaat
     8221 gttgatatgg cagcaaatac gaaagtacgt attgaaggtt ctaaaaacct aattgatgcg
     8281 gcgaaaaagc atgacgttaa gaaagtaatt gcccaaagta ttgcctttat gtatgaacct
     8341 ggcgaaggat tagcaaatga ggaaacttca cttgatttta actcaactgg cgatagaaaa
     8401 gtaacggttg atggtgtggt tggtttagaa gaagaaacgg ctcgtatgga tgaatacgtt
     8461 gttttacgtt ttggctggtt atatggccca ggtacttggt acggaaaaga tggcatgatt
     8521 tataatcaat ttatggatgg tcaagtgaca ctttcagatg gcgtaacatc atttgtgcat
     8581 cttgatgatg cagttgaaac atctattcaa gctattcatt ttgaaaatgg tatctataat
     8641 gtagcagatg atgcacctgt taaaggttct gaatttgcag aatggtataa agaacaactt
     8701 ggtgttgaac caaatattga tattcaacct gcgcaaccat ttgaacgtgg cgtaagcaat
     8761 gagaagttta aagcgcaagg tggtactctg atttatcaaa cttggaaaga tggcatgaat
     8821 ccaattaaat aataatttat ccgtttaata tacaaagaat aaagacttgg tcgaatcgtg
     8881 gatgatatat tatcaaacgc acggctcgaa caagtctttt ttattatgtc ttcgttatct
     8941 ttgtatgaag gaataacaga attacaatta atgtactgaa taatgcaatt aatgttgtga
     9001 ttagtgctaa tttaatttct attggtagcc aagtcagtac aaaagaccaa ttattgctac
     9061 cgagaatgag atatggtaat gcatataata tgagcgctaa agcgatacat atacataatg
     9121 ataaccaact caatacagca atccgttttg atctacgcaa aagaatttga ccatggcgca
     9181 tttgccaagc acgataagct gagaatataa atgctaataa aataagtgtt gtcatcaaaa
     9241 cggtaacaat attaaattga tctttatatt gattgagcat agacgcaacc gtcgaatatc
     9301 gcttgtgatt tacaatttgt gtattaagat gctcaactaa tttgggtaca tattccgaat
     9361 ttagatttgc aagtacaaca attccataat tttgttttgg atttagtaaa ataaatgatg
     9421 aaaagttatc tagcgttcct gaatgaaaca ctaaatgttc atcattattg gtaaaccagc
     9481 cggaagcata tgcattggca ttaggttcac caattgttga agataaattt ttatgtgatt
     9541 gttgaactaa tgatttgtat ttatcaggtg gattaagttg gaattttatc caatgttcca
     9601 aatcttcagt tgatgtcatc atatatgctg atggtgtatc ccaaaggtta aattcaggtt
     9661 tagagacgac aggtgtcgaa ccttgtaatt catagccaat agcatcatgt tttgatttgt
     9721 aattggtttg tttgaatgat gtatgtgtca tatgcaaagg cttgagccat gaatttgtaa
     9781 tatattttgt ataggattgc ttcgtaacgt tttggataat taaacctaat aaatcatagt
     9841 tcatatttga gtattcaaat tcttctccgg gcttatgatg taattcatca cccataattg
     9901 cacgggttac atcatttaaa cgattatttt tgcttgtcac agaatcttcg cttgtaatat
     9961 cactaggtat accacttgtt tgagccaaaa gttgcttaat cgtaatagtt tcattttgac
    10021 cattatagtt cattttaaaa tgaggcacat gtttggatac ggcatcattt aagtttaatc
    10081 gaccttcttg agctaatttt aaaattgcaa gacctgtgaa agctttcgta ttagaagcaa
    10141 tttcatactt tgttgtggga gaagctttga cttttttatc aacatcttga taaccataac
    10201 ctttatttaa aaaaactttg ccatttttta ctattaaaat tgatgctcca ggaatgtgtc
    10261 ccttttgtaa atcatgctcg ataattgtat ctatttgttg ttgcgaatca ttggttaacc
    10321 gtgtcttcgt attgctattt aatgttatat atatagcaat tgaaatggcg actaaaatga
    10381 taatagaaat ggatagaaaa tacagttttt tagtagtcat aaacgttcct ttttatatgt
    10441 taaatttact taagtgccta atgagattta ttttattata ccagttctat tgaataaatt
    10501 aattaaagag gtatatttac cattattttt gacaccttat atgttaatag gtgtataatt
    10561 acataaaaaa tattaattaa ctctctatac tttttggaaa gataggcgta tagtgatatc
    10621 catcaaaaac tataaaaagg aataggagat tatgttcaat caatttaaaa gacttattat
    10681 agggcaacct aaaaaaaaca gagaactaaa agacgaaaag atatcgaagt ttaaagggtt
    10741 agcgattctt tcgtcggatg cattatcttc agtggcttac ggtcccgaac aaatactgat
    10801 tacactatca gtagtgggtg cagttgcaac ttggtatact ttaccgattg caggtgctgt
    10861 tctgatctta ttagctgctt taattatgtc ttataggcaa attatttatg catatcctaa
    10921 aggtggcggc gcgtatatgg tgtccaagac caatttagga gaaaaatggg gattgcttgc
    10981 aggaggctca ttattagtcg attacatact gactgtcgct gttagtatat catctggtgc
    11041 tgatgcattt gttgctgcat tcccaagttt atatgggcat aaagtactga ttgcatgttt
    11101 acttgtactt tttattttaa tattaaattt acgtggttta actgaatctg ccaccgtatt
    11161 atcctatcca gtatatcttt tcattatagg gttagtgata ttaatattta taggtacttt
    11221 ccgtgtggcg acaggagata ttcaaccaca tatgcatgca tcagtaggaa ctgcggttcc
    11281 tggagtaaca ttattcttgc tattaaaagc attttcatca ggggcttcat cgttaacggg
    11341 tgttgaagct atttcaaatg cggtaaccaa ttttagagag ccaagtgcta aaaatgctgt
    11401 gaaaacgtta attgctatgg gttcaatttt agcgttttta ttagttggta tcgttggttt
    11461 agcatatgtt tatggaattt tgccacaaac ggaaacgaca gttttatcac aattagctat
    11521 gcaaatattt ggtgataatg ctgccttcta ttttgtacag gcaacgaccg tgatgatttt
    11581 ggtattagca gccaacactg gatttacagc ttttccgatg ttagcggctt caatgtctaa
    11641 agataaatat atgcctagaa tgtttactgt tagaggtgat cgcttaggct actctaactc
    11701 gattattatt ttaggtgtat tagccattat tttaattatt gtatttgatg gtatgactga
    11761 agatttaatt ccattgtatg cggttggtgt atttattcca tttacactag cacaattcgg
    11821 tatggttatt aaatggattc atgaacgtcc taagaattgg ttaagtaagt tgtctgtgaa
    11881 cttacttggt ggtatcgtga catttattgt ttttatgatt ttactcataa ctaaatttag
    11941 ccaagtatgg ccgatactta ttttcttgcc gttcgtggtg attttcttct tgaaaattaa
    12001 caaacattat cgagatatcg cagaacaatt acgttctgac attgatgttc ttaatgtgga
    12061 cgtggtagat cgtaatttag caattgtgcc gataacaagt attacaacag cagttgataa
    12121 gtctatttat tacgcacaaa tgcttgcgaa taatgatgtc atagcagtac atgtatcatt
    12181 tggagatgaa gatgaaaaag cattccaaga gaaatggaaa cgtcatttcc cagatgtaag
    12241 attagttatt ttacattcgg aatatcgcag tattatacga ccaatttcac gttttatcga
    12301 taagattaat cgtaaagcca acgatcaaaa ctatatgatt acagtagtga taccagaatt
    12361 tattactaag aaacgttggc ataatttatt acacaatcaa actagtttgc gtatgaaatt
    12421 gtatttaatt tatcaaaaga atgttaacgt atgtacaatt ccatttaaac ttaaaaaata
    12481 gattctataa tcaaatcgga ttgatgaaag gttatttact tttcatcaat ctgatttttt
    12541 gatgttgaat ataaaaaccc cgtacatatg agatgataat gactttgtct catatgtacg
    12601 ggggtattgt tatgtcttgc ttaatgtttg gactgtaaag acaagacaat ttcgttgtaa
    12661 ttgatatttt ctcgaatttg agctgcaacg atattgttga ttttattttt gcgaattaat
    12721 tcatctaaaa gatgacgttg taagtacaat gcttccaatt tgtggttatg gaacatagct
    12781 tgcggtatga tttgattgct gttttcttga cgttcttttc gacgttgtgc acgccacttt
    12841 aatgagcgac tcatatcata gtaatgatta atgactaagc taacctcaag tacatttgta
    12901 ctattttgtt cttcttttaa gcgtaaaata atattgtgat tcacaatacg cataattttt
    12961 tgaacatctt tgaatgaatt ttcaaattct tgcttcttag attctttatt taaggctttg
    13021 tagtgcttca tcgctttaag tctaagccat aacatcttga agaagtttgc aaatttttca
    13081 acaaatgatg cagtacggtg tgtctctgtt aattcgacaa tattgcggta attattaata
    13141 tcctgatatg ttgccttacc tttatcaatt aaacgctcaa gtgtagatgt ttcgattact
    13201 tttgcaatat cttctaaacg tttgagttct tttgtatttt gattatcagg ttctgaattt
    13261 aataaaaata acaattctcc atagtattgg tttaatactg ggcgataatt tgtatcgttt
    13321 ttgtcttttt tactttcgtt tttaaaatgc tggatcactt tttgaacaat gaaaattttg
    13381 gcagattgat aagtcatacc tttaaaagta gtatcttcgg cagatggtgt aattaaaggt
    13441 aaaacaattt gcgctaagat taaactaatt aataccatga aagatgcaat aaacaataaa
    13501 tcattacggt attcgaatgc ttgtcctttt gtaatgataa atggtaatgt aagtgccatt
    13561 gaaagtgaaa ttgtaccgtg aataccacac atggtcataa taaatgcgta acgcactcga
    13621 gaaggtggtg tttcatgtga atcatgttcc tcgtctagat aagattgtat atttttcggg
    13681 aaataaaaat ctttgtacca gaaataaacc caaacaaatc gacatgcata aattgcaata
    13741 gcgattaata tagtgatcac tattaaaaat gaaaagttgt caggctctgt ttggaaaata
    13801 tctatgacta cagtaggaat cataaaacct aatacaacaa atacaaaacc atttaaggca
    13861 taactaaatg tgttccaaat ttgatggtaa ttcatttgta gttccgtttg tgctctaatt
    13921 aaacggtcgc gttctaaacc atggattaga cctgcgatta caactgcaat gatacctgaa
    13981 gcatgaactt cttctgctaa aaagtatacg acaaaaggag ttaataattg aataaaagtt
    14041 aaggtattgt tatcttttaa acctttatta gcggttaagt ctatacgtat tctaacgaca
    14101 acgaatccaa taattgcacc aataagtaca cctagtattg ttgaaatgat aaattgttca
    14161 acagcttgga ataatgaaaa ggtaccagtt actaatgcag taacagcaat tttaaatgaa
    14221 atgatacctg ctgcatcatt gagtaaagat tcaccttcta aaatcgtcat agaaccttta
    14281 ggtaataatt ttccgcgtgt aatagcagat actgctactg catcagtagg acataaaatt
    14341 gctgctattg caaaagcggc tggcattggt aaggcaggcc aaatccaatg tataaaatag
    14401 ccaacaccga ctacagttgc aaacactagt gccattgaca ttaatagtat aggtttacga
    14461 tattctaata attttgttcg agagacgtgg gtaccttcca caaaaagtag tggcgcgata
    14521 acggcaaaca taaatacttc agaattgaat tggaaatcaa cttgtattgg aataatgaaa
    14581 ataacgacac ctaatgcaat ttgaataaag gcagtaggaa tttgtgggaa tcgattattg
    14641 ataaccgaac taataatcac agcaaaaata aaaattaaaa atgcttctaa tagtgccata
    14701 caatactcct caaaatttta atagttaata ttttatcact tttaaggcat aatgacatag
    14761 atatattgat aaaatgaagt tattttcaaa aaaactctag tatcggttga actgatacta
    14821 gagcgagatg tttaaattat tgattgtcat atctgaaatg accgctgtca ttttgtcgtt
    14881 gttcatacgc gagcttttca gcattcgttt tgtatttttt ataaaagaaa aataaaaata
    14941 taaaccagaa tggcgaaata taaatagctg ctcttgtttc gtcactaaag aataataaaa
    15001 tgaatacaaa gaagaagaac gctagaataa tgtaagcaat aggcttacca ccaatcaact
    15061 taaatttact gtttttatgt gcctcaggat gctttttcaa atacattaga taagcgacaa
    15121 taatcattgc ccaaacaact aaaaacaaca cagttgataa cgttgtaacg tatatgaata
    15181 gttgaattgc attagggaaa atgtagttta acaaggctgc tataagtaat gcaattgatg
    15241 aaactaacat tgataaataa ggcacgccat gactattcgt tttatttaaa actttaggac
    15301 ctaacccttg ttgtgacagt ccgaataaga tacgactatt cgaatagata ccactatttg
    15361 tagcagaggc cgcggcagtt agcacgacaa agttaacgac acctgctgca aatggtacgc
    15421 cgattaatgt aaagagttta acgaaagggc tactatttgg atcgatatca ttccaaggta
    15481 tgactgacat aattactaat agaccaccga tatagaataa taaaatacgg ataggtacat
    15541 tattaattgc tttcggtaag gttttttcag gatctttcgt ttcaccagca gttacaccta
    15601 taagttcaat accaatgaat gaatatacag caatttggaa tgacattaag aaaccaaatg
    15661 ttccaccagg gaacatgcca ccgtgactga ttaagtttgt gaatgatgca tgtccataat
    15721 gtgttttaaa tgagaaaaag attaatacaa gaccaacgat aaccatcgca ataattgtga
    15781 caactttaat aatcgagaac caaaactcca gttcaccaaa taatctggca cctaataagt
    15841 tgaagctaat caagattaaa acaataaata gcacggtaat ccaatttggg acttgtggat
    15901 accaaaatgc aaagtattgt cccatcgcag ttaggtctga catacttgat acaatccaac
    15961 ataaccagta agtccagcca atgacaaagc caccaaaagg gcctaaatat tcatttgcaa
    16021 tatcaacaaa tgaattaaat cttgtattgc tcaacaacaa ttcgcctaat gcgcgcataa
    16081 aagcgaatag tacaacccca ataatcatgt atgtgaataa cagtgatgga ccagttaaag
    16141 atattgtttg acctgatcct aggaataacc cagttccaat tgcgccccca attgctatta
    16201 attggatgtg tctgttattc aactctctat gcaattttct tgccataatt ttcctcctca
    16261 ggataaagca aataaaaaag ttgtgtgtaa gcgttttctt aattataaat actaaagaaa
    16321 tccatttaat aaagcaatta tttttttaaa tataagaaaa aacatttgat aggtaagggt
    16381 atgataacta aaacttatcg attttttgaa aagatataag tttttatgca aaaatataaa
    16441 ataatcatct aaatgaagca gttaactata tatattagat aagctatggc ataataaata
    16501 acattttaat ttgagtaaag aaggtaaggg tatgaagata gggacaatcg ccgatttaca
    16561 tattgatcgt cataataaaa aaacatccga agattactta gaggcattgg ttgaaatagt
    16621 taagtataag aaattggata tattgttgat tgcgggggat atctcaaatc attatcaatt
    16681 gacgcaccaa tttataacgc aattgacaaa acaattagat atacctgtga aatttgtacc
    16741 tggaaaccat gatttatggg aagttgaaag tatgactacg caagacattt ggaataatta
    16801 taagagtatg tcacagtgct tggtaggaaa accatttata gtaaatgaag aatgggcaat
    16861 cataggacat actggctggt atgattatag ctttgcagca caacgatttt cattagatga
    16921 gttacaaaaa ggaaaacatt atggtgcgac ttggcaagat aaagaacgaa tatcttgggg
    16981 catatcagat caaaatttat ctaaaatagc ggctgaacaa gtgaagaaag atatattaga
    17041 agtaggaaat agacgagtga ttttagtcac acatgttgtg acgcaccctg atttcattgt
    17101 tcctatgccg catcgtatat tcgattttta taatgcattt attgggacaa gtgatttcaa
    17161 tcctttgtat gcgatgttcg atataccata tagtattatg ggccatgttc attttcgtaa
    17221 aagtgtgata gatgatggca gatgttatct ctgtccgtgt ctaggctatc caagacaatg
    17281 gcgttcagaa gatatttacc aggaaataaa tgagacgata caaataatag aaatttaaaa
    17341 tgcgcaaacc tgacccagtt tgcgcatttt atgttttaca cacgcgagta atgtgtttac
    17401 ttacgtgtgt ttattttgtt gctgattttc aattgtatat gaatgtggtt gcacataaat
    17461 gcactttctt cctggtgaat taaagctgta ttccattttc tctttacgaa ttttaataat
    17521 ttgtttgcga tttggaatga tggcaggtaa aactaggcca cgacgaatat gactccaaag
    17581 tggatcgata taatagtaat agtaatcatc gtacccaatg agtaacgtta cgtgaatatt
    17641 tgaaacttgt ttcgctggct tgttatcaaa gcggaaaaca cgtagtaatg gtttagaacc
    17701 aagattagta tggtatatta acacaggttg accttgatcg ataatacctt taagatcttc
    17761 taacgattta ccagtgccgt ctacgatatt aggattgtat ttttgtaaaa atggtacata
    17821 tgcttctgga aatatcgttt gatgataatt gccaagctta atgaataagt gatgtccaac
    17881 ataaccttta tgtggattgt tcggatgtgt cggccaatgt ctcataattt ctgtagcagg
    17941 gatatgttgg ttgttgtatt gcaacatcat ggctgcggaa acaccttcac accccatgac
    18001 catagggata ggaaatagct gactgatagg tttaactggt aatatttttc ggttcataat
    18061 atagtcctcg cattgattca ataaatattt aatataatta tatagcgtca atgcaaaatg
    18121 tcctaaacat atgttttaca tgagtgaata aaattaatgg agtgataaaa tggaatatca
    18181 attacaacaa cttgcgtcgt taacgttagt aggtattaaa gaaacgtatg aaaatggacg
    18241 acaggctcag caacatatag cagggttttg gcaaagatgt tatcaagagg gagtaattgc
    18301 ggatttacag ttaaaaaata atggtgattt agccgggata cttggcttat gtatacctga
    18361 attagacggt aagatgtcat atatgattgc agttaccgga gataatagtg ctgatattga
    18421 aaaatatgat gtcataacat tagcaagttc aaagtatatg gtatttgaag cacagggcgc
    18481 agtacctaaa gcagttcaac aaaaaatgga agaggttcat cactacatac atcaatatca
    18541 agcagatacg gtaaaatcag caccattttt tgagttgtat caggatggtg atactacaag
    18601 tgaaaaatat attacagaaa tttggatgcc tgttaaaggg tgattgaata gaaatgtcct
    18661 tgctatgaag gcgtaatttt agttgaagat tcgatctcat aattcataaa gaagaaccct
    18721 gacaaagata gatatctttg ctagggttct aattatgttg aagtgttatg cgacatgttg
    18781 ctttttaatc attttaaagt aaatgatttg tagactgact aatagaatgg ctgtaatgaa
    18841 gataataatc atatatggtg atgtattaaa ttcgcctttt aagccaacta atggtgcaac
    18901 agcgccacct aagataaatt ggaataagcc aagtaaactt gatgcgttac cactgccacc
    18961 tgttcgttct tccatagcca ttgtgaaacc aagcggtcca attgacgtca caggacacac
    19021 atttaagaag aatgcgatga gtaagaccca aagtggtaaa tggaatgtaa gtgtcaggat
    19081 aattaaagca acacctacca cttgtataat agttaaaatg attaataata tgtggcgatg
    19141 taatttttct actaataaag caacgacttg actgacaatg attaaaccta caccgttaac
    19201 agcaaacatg atactaaatt gttggggtgt catattatac aatttttgag taataaatgg
    19261 cgatgcagat gaataactaa atagcattac ataagttaaa ccttgtaata gcattggaat
    19321 aataaatgct ggttttttca aaagacttcc aaaatcttta atgacgctac taaaattcac
    19381 ctgctttaat tcatctttag atgttttagg taattgagaa gcgacgccaa ttaaaatgat
    19441 gagtgccaca atagttaaaa ttgtgaaaat agaacgccat gttgctacgg ataaagctaa
    19501 tccaccggct aatggtgcaa gaatagtgat gatgccatta actaccatta aagatgctaa
    19561 aaatttagcg agtgcgttgc cactaaattt atcaccagca gaggcttttg caatcacgat
    19621 gacgccacca ccagttaaac cttgaataaa tcgtaaagtt aagaataatg gcaattgatc
    19681 aacaaacata gacaaacctg ataccaaaat gaaaatgatc aaaatggata cagcaatccg
    19741 tttgcgacca aaagcatcgg ataaaggtcc aaagataaat tggcctagtg caagaccaat
    19801 cattgtgaat gataatgtta attgaatttc tgatgtagta gaaccaaaat caccttgcac
    19861 tttaggtaat gatggtacgt acatatctat agttagtggg ccaaaagcgg tcatgacgcc
    19921 taacgtaatt agcaataaaa atgaaaaatg cttcgttttg gttttcgttg attgcatgat
    19981 cagactcctt taaatgata
//
LOCUS       DAKTIO010000031        19876 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.031, whole genome shotgun sequence.
ACCESSION   DAKTIO010000031 DAKTIO010000000
VERSION     DAKTIO010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19876
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.031"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(153..743)
                     /locus_tag="O0293_000851"
     CDS             complement(153..743)
                     /locus_tag="O0293_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCY0279745.1"
                     /translation="MKVNPNNIELIISAVKEEQYPETELSEVALSGRSNVGKSTFINS
                     MIGRKNMARTSQQPGKTQTLNFYNIDEQLIFVDVPGYGYAKVSKTQREKFGKMIEEYI
                     TKRENLQLVIQLVDLRHDPTQDDILMYNYLKHFDIPTLVICTKEDKIPKGKVQKHIKN
                     IKTQLDMDPDDTIVSYSSIQNNKQQQIWNLIEPYIS"
     gene            complement(897..2159)
                     /gene="clpX"
                     /locus_tag="O0293_000852"
     CDS             complement(897..2159)
                     /gene="clpX"
                     /locus_tag="O0293_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HCY0279746.1"
                     /translation="MFKFNEDEENLKCSFCGKDQDQVKKLVAGSGVYICNECIELCSE
                     IVEEELAQNTSEAMTELPTPKEIMDHLNEYVIGQEKAKKSLAVAVYNHYKRIQQLGPK
                     EDDVELQKSNIALIGPTGSGKTLLAQTLAKTLNVPFAIADATSLTEAGYVGDDVENIL
                     LRLIQAADFDIDKAEKGIIYVDEIDKIARKSENTSITRDVSGEGVQQALLKILEGTTA
                     SVPPQGGRKHPNQEMIQIDTTNILFILGGAFDGIEEVIKRRLGEKVIGFSSNEADKYD
                     EQALLAQIRPEDLQAYGLIPEFIGRVPIVANLETLDVTALKNILTQPKNALVKQYTKM
                     LELDDVDLEFTEEALSAISEKAIERKTGARGLRSIIEESLIDIMFDVPSNENVTKVVI
                     TAQTINEETEPELYDAEGNLINNSKTSA"
     gene            complement(2310..3611)
                     /locus_tag="O0293_000853"
     CDS             complement(2310..3611)
                     /locus_tag="O0293_000853"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCY0279747.1"
                     /translation="MTATWEKKEGNEGLLTVTVPAEKVNKALDQAFKKVVKQINVPGF
                     RKGKVPRPIFEQRFGVEALYQDAIDILLPDAYGEAIDETDIKPVAQPEVSVTQIEKGK
                     DFIFEATVTVEPEVKLGDYKGLEIEKQETELSDDELQEAIDHSLGHLAEMVVKEDGVV
                     ENGDTVNIDFSGSVDGEEFEGGQAEGYDLEIGSGSFIPGFEEQLEGMKVDEEKDVVVT
                     FPEEYHAEELAGKEATFKTKVNEIKFKEVPELTDEIANELDAEANTVDEYKENLRKRL
                     AEQKATDAENVEKEEAITKATDNTTIDIPEAMVNTELDRMVSEFAQRIQQQGLDLQTY
                     FQISGQDETQLREQMKDDAEQRVKTNLTLTAIAEAEKIEATDEDIDKELEKMSKQFNI
                     SVEDIKNTLGNTDIIKNDVRIQKVIDLLRDNAKFVEGTKED"
     gene            complement(3659..3769)
                     /locus_tag="O0293_000854"
     CDS             complement(3659..3769)
                     /locus_tag="O0293_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279748.1"
                     /translation="MQNNTQHFNIVLTKKVDLQIYKRCDFNVCIGNRPMI"
     gene            complement(3774..4703)
                     /locus_tag="O0293_000855"
     CDS             complement(3774..4703)
                     /locus_tag="O0293_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279749.1"
                     /translation="MSDIIQFPNSSKKLYKDIKRAEQDQNYDLMYEYIVQYERQFELT
                     EEIAMMKCRMLYETGSFLELREETIVLLKTGIQQYDALMIYYVKSLIGLGQYFEAVEV
                     IHQIIDEVKDHKTRMALHPLKEFAKSKLIEDEKRLTQSLADFDTLSMREQTHLILKLI
                     DNGHFQFQETVLYILKSNTYSYNLISLMIEYLRFANCTQELTIEKYGMDVTFVPANLK
                     GLEHTTLKEKVIPNVMETLNDGALHIAEEAHHIMNNHSIMIYPIDIETLFETNKWINA
                     YECYFKNMLGLKCELQSIDAFNFIQQLDLNNNS"
     gene            complement(4722..5330)
                     /locus_tag="O0293_000856"
     CDS             complement(4722..5330)
                     /locus_tag="O0293_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCY0279750.1"
                     /translation="MSKFDEQIIVVPREIIFNNEKNTFNGFLNKNKPEGQNIFDALSQ
                     YEVKRRGDMEEDPSYKQLISYCLLENEHGEILVYERLSGGGEARLHGQSSIGVGGHMN
                     DVPGAESINEVLRVNAQRELEEEVGLSEQDSQNMEYIGFINDDNNEVGKVHIGVVFKI
                     TVSTNDVEAKETDTLRIKWVEKGNIESYDDFETWSALILQDL"
     gene            complement(5472..5828)
                     /gene="rplT"
                     /locus_tag="O0293_000857"
     CDS             complement(5472..5828)
                     /gene="rplT"
                     /locus_tag="O0293_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCY0279751.1"
                     /translation="MPRVKGGTVTRARRKKTIKLAKGYFGSKHTLYKVAKQQVMKSGQ
                     YAFRDRRQRKRDFRKLWITRINAAARQHEMSYSRLMNGLKKAGIDINRKMLSEIAISD
                     EKAFAQLVTKAKDALK"
     gene            complement(5875..6075)
                     /gene="rpmI"
                     /locus_tag="O0293_000858"
     CDS             complement(5875..6075)
                     /gene="rpmI"
                     /locus_tag="O0293_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCY0279752.1"
                     /translation="MPKMKTHRGAAKRVKRTASGQLKRSRAFTSHLFANKSTKQKRQL
                     RKARLVSKSDMKRVKQLLAYKK"
     gene            complement(6104..6631)
                     /gene="infC"
                     /locus_tag="O0293_000859"
     CDS             complement(6104..6631)
                     /gene="infC"
                     /locus_tag="O0293_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCY0279753.1"
                     /translation="MSTIAKDQTQINDKIRAKELRLIGQDGEQIGVKSKREALEMAER
                     VDLDLVVVAPNAKPPVARIMDYGKFKFEQQKKEKEMKKKQKIINVKEIRLSPTIEEHD
                     FQTKLKNGRKFLTKGDKCKVSIRFRGRAITHKEIGQRVLEKYADECKDIATVEQKPKM
                     DGRQMFIMLAPTAEK"
     misc_feature    complement(6660..6785)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(6860..8353)
                     /locus_tag="O0293_000860"
     CDS             complement(6860..8353)
                     /locus_tag="O0293_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0279754.1"
                     /translation="MSKVQNESNNVVKRGLKDRHISMIAIGGCIGTGLFVTSGGAIHD
                     AGALGALIGYAIIGIMVFFLMTSLGEMATYLPVSGSFSTYATRFVDPSLGFALGWNYW
                     FNWLVTVAADITIAAQVIQYWTPLQGIPAWAWSALFLVIIFSLNSLSVRVYGESEYWL
                     ALIKVVTVIVFIAIGLLTIVGIMGGHVVGFEIFNKGEGPILGGNLGGSLLSILGVFLI
                     AGFSFQGTELIGITAGESENPERAVPKAIKQVFWRILLFYILAIFVIGMLIPYDSSAL
                     MGGSDNVATSPFTLVFKNAGFAFAASFMNAVILTSVLSAGNSGMYASTRMLYSMSKDK
                     LAFEAFGKTNKNGVPYMSLLVTAIIVVIIFVLQSLTKGAYEYIVAASGLTGFIAWVGI
                     AVSHYRFRRAFDKQNYDKSKLKYTAKLFPFGPLFAGFLCIVVIIGQDVDFIKTGDFNF
                     SRFLITYMGIPVFLAFFIYHKLRYKTKKIPLEQVDLRQDVSMDEIKG"
     gene            complement(8779..10716)
                     /gene="thrS"
                     /locus_tag="O0293_000861"
     CDS             complement(8779..10716)
                     /gene="thrS"
                     /locus_tag="O0293_000861"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCY0279755.1"
                     /translation="MEQINIQFPDGNKKAFDKGTTTEDIAQSISPGLRKKAVAGKFNG
                     QLVDLTKPLETDGSIEIVTPGSEEALEVLRHSTAHLMAHAIKRLYGNVKFGVGPVIEG
                     GFYYDFDIDQNISSDDFEQIEKTMKQIVNENMKIERKVVSRDEAKELFSNDEYKLELI
                     DAIPEDENVTLYSQGDFTDLCRGVHVPSTAKIKEFKLLSTAGAYWRGDSNNKMLQRIY
                     GTAFFDKKELKAHLQMLEERKERDHRKIGKELELFTNSQLVGAGLPLWLPNGATIRRE
                     IERYIVDKEVSMGYDHVYTPVLANVDLYKTSGHWDHYQEDMFPPMQLDETESMVLRPM
                     NCPHHMMIYANKPHSYRELPIRIAELGTMHRYEASGAVSGLQRVRGMTLNDSHIFVRP
                     DQIKEEFKRVVNMIIDVYKDFGFEDYSFRLSYRDPEDKEKYFDDDDMWNKAENMLKEA
                     ADELGLSYEEAIGEAAFYGPKLDVQVKTAMGKEETLSTAQLDFLLPERFDLTYIGQDG
                     EHHRPVVIHRGVVSTMERFVAFLTEETKGAFPTWLAPKQVQIIPVNVDLHYDYARQLQ
                     DELKSQGVRVSIDDRNEKMGYKIREAQMQKIPYQIVVGDKEVENNQVNVRQYGSQDQE
                     TVEKDEFIWNLVDEIRLKKHR"
     gene            complement(10914..11036)
                     /locus_tag="O0293_000862"
     CDS             complement(10914..11036)
                     /locus_tag="O0293_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279756.1"
                     /translation="MFDENMVNKLDDYYFIYGICNEYPDQDRYLKQRYLIHKLY"
     gene            complement(11129..12049)
                     /gene="dnaI"
                     /locus_tag="O0293_000863"
     CDS             complement(11129..12049)
                     /gene="dnaI"
                     /locus_tag="O0293_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HCY0279757.1"
                     /translation="MKQFKSIINTSQDFEKRIEKIKKEVINDPDVKQFLEAHRAELTN
                     AMIDEDLNVLQEYKDQQKHYDGHKFADCPNFVKGHVPELYVDNNRIKIRYLQCPCKIK
                     YDEERFEAELITSHHMQRDTLNAKLKDIYMNHRDRLDVAMAADDICTAITNGEQVKGL
                     YLYGPFGTGKSFILGAIANQLKSKKVRSTIIYLPEFIRTLKGGFKDGSFEKKLHRVRE
                     ANILMLDDIGAEEVTPWVRDEVIGPLLHYRMVHELPTFFSSNFDYSELEHHLAMTRDG
                     EEKTKAARIIERVKSLSTPYFLSGENFRNN"
     gene            complement(12049..13449)
                     /locus_tag="O0293_000864"
     CDS             complement(12049..13449)
                     /locus_tag="O0293_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HCY0279758.1"
                     /translation="MGRQAFEFGLRPKDQFKVMQHFDLNTNHLEVLNRLYTPLIGTQA
                     VGLYHFMTQFVKESHNETLILSHYIFMNELKINLLEFRQQMDLLEAIGLLKAFVKHDE
                     QETQFVYQLIQPPSAHLFFNDPMLSIFLYSEVEHRRFHELKKYFEYQQIDLSEFKQVT
                     RQFTDVFKVPSTKIDVDTSDIPINEPYQGIDLSNESFDFEMLRQMLGKHFISQDIVTK
                     DAKRLITQLATLYGLTADGMKHVILNSITSGQQLSFEEMRKQARSYYLMEHENQMPKL
                     QVKSPATSSSAGKSSEVNPKPQSDEWFELLEQTSPIDMLASWSESEPTISQKTMVEEL
                     IEREKMSFGVINILLQFVMLKEDMKLPKAYILEIASNWKKKGIKTAKEAYNYAKKVNQ
                     PKNEGSSGNYQKRGSYYGQRNRISKEKTPKWLENRDKPSEEDSAKDNSVDDQQLEQDR
                     QAFLDKLSKKWEEDSQ"
     gene            complement(13450..13920)
                     /gene="nrdR"
                     /locus_tag="O0293_000865"
     CDS             complement(13450..13920)
                     /gene="nrdR"
                     /locus_tag="O0293_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HCY0279759.1"
                     /translation="MKCPKCNSTQSKVVDSRHADELNAIRRRRECENCGTRFTTFEHI
                     EVSQLIVVKKDGTREQFSREKILNGLVRSCEKRPVRYQQLEDITNKVEWQLRDEGHTE
                     VSSRDIGEHVMNLLMHVDQVSYVRFASVYKEFKDVDQLLASMQGILSENKRSDA"
     gene            complement(14130..15155)
                     /gene="gap"
                     /locus_tag="O0293_000866"
     CDS             complement(14130..15155)
                     /gene="gap"
                     /locus_tag="O0293_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCY0279760.1"
                     /translation="MSTNIAINGMGRIGRMVLRIALQNKNLNVVAINASYPPETIAHL
                     INYDTTHGKYNLKVEPIENGLQVGDHKIKLVADRNPENLPWKELDIDIAIDATGKFNH
                     GDKAIAHIKAGAKKVLLTGPSKGGHVQMVVKGVNDNQLDIEAFDIFSNASCTTNCIGP
                     VAKVLNNQFGIVNGLMTTVHAITNDQKNIDNPHKDLRRARSCNESIIPTSTGAAKALK
                     EVLPELEGKLHGMALRVPTKNVSLVDLVVDLEKEVTAEEVNQAFENAGLEGIIEVEHQ
                     PLVSVDFNTNPNSAIIDAKSTMVMSGNKVKVIAWYDNEWGYSNRVVDVAEQIGALLTS
                     KETVSAS"
     gene            complement(15325..15948)
                     /locus_tag="O0293_000867"
     CDS             complement(15325..15948)
                     /locus_tag="O0293_000867"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCY0279761.1"
                     /translation="MPKVIGLTGGIASGKSTVSELLSVFGFKVVDADKAAREAVKKGS
                     KGLAQVREVFGDEAIDENGEMNRRYMGDLVFNHPEKRLELNAIIHPIVRDIMEEEKQE
                     YLKQGYNVIMDIPLLFENELENTVDEVWVVYTSESIQMDRLMQRNNLSLEDAKARVYS
                     QISIDKKSRMADHVIDNLGDKLELKQNLERLLEEEGYIEKPNYGEED"
     gene            complement(15964..16836)
                     /gene="mutM"
                     /locus_tag="O0293_000868"
     CDS             complement(15964..16836)
                     /gene="mutM"
                     /locus_tag="O0293_000868"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCY0279762.1"
                     /translation="MPELPEVEHVKRGIEPYVINQKIEHVIFSDKVIEGKAQGKETII
                     KGIELDTFKTLSEGYTITNVERRSKYIVFQLDNKREQRTLISHLGMAGGFFIVDELED
                     IMIPNYRKHWHVIFELSNDKKLIYSDIRRFGEIRNVASVASYPSFLEIAPEPFSNEAL
                     TYYLNRIHQQSNKNKPIKQVILDHKVIAGCGNIYACEALFRAGVLPDKKVKDLTHQQQ
                     EMVFYYVREVLEEGIKYGGTSISDYRHADGKTGEMQLHLNVYKQPVCKVCGSQIETKI
                     IATRNSHYCPVCQK"
     gene            complement(16852..19482)
                     /gene="polA"
                     /locus_tag="O0293_000869"
     CDS             complement(16852..19482)
                     /gene="polA"
                     /locus_tag="O0293_000869"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCY0279763.1"
                     /translation="MNKLVLIDGNSLSFRAFYALPLLSNKAGIHTNAVYGFAMLLEKI
                     LKEEKPNHFLVAFDAGKTTFRHEKYSEYKGGRQKTPPELSEQFPYIRQLLDAYHIKRY
                     ELDNYEADDIIGTLSKEADKAGFQTIIITGDRDLTQLATDNVTIYYTKKGVTDVDHYT
                     PDFIAEKYNGLTPNQIIDMKGLMGDTSDNIPGVAGVGEKTAIKLLNQFDTVEGVYEHL
                     DEISGKKLKEKLQNSKEDALMSKELATINVDSPIEVKLEDTLMTHQDEQQEKIELFKK
                     LEFKQLLADIDQSASVEDAIEKTFEIETSFDNIDFTSLKEAAIHFELDGGNYLRNNIL
                     KFSLFTGEKHIVINADDINNYVELVSWLENPNSKKVVYDAKKTYVASHRLGIDIQNIS
                     FDIMLASYIIDPSRTISDVQSVVSLYGQSFVKDDVSIYGKGKKFKVPEDDVLNPYVAS
                     ITDAIYFAKPNMDKQLEEYNQVELLADLELPLAKILSEMEEIGIFTDVHDLEEMEKEI
                     QEKLDVLIRNIHDAAGEDFNINSPKQLGVVLFETLQLPVIKKTKTGYSTAVDVLEQLQ
                     GEHPIIDYILEYRQLSKLQSTYVEGLQKVISDDQRIHTRFNQTLAQTGRLSSVDPNLQ
                     NIPVRLEEGRKIRKAFKPTSKDSVILSADYSQIELRVLAHITQDESMKEAFINGDDIH
                     TATAMKVFGVEADQVDSLMRRQAKAVNFGIVYGISDYGLSQSLGITRKKAKAFIDDYL
                     ASFPGVKQYMSDIVKDAKALGYVETLLHRRRYIPDITSRNFNLRGFAERTAMNTPIQG
                     SAADIIKLAMVKFAQKMKETTYQAKLLLQVHDELIFEVPKSEVDSFSEFVEEIMENAL
                     QLDVPLKVDSSYGATWYDAK"
BASE COUNT         6003 a         3809 c         2685 g         7379 t
ORIGIN      
        1 acgatgctta aataaattat aatctttcta atttactttt gagtttttga caaaatgatg
       61 aacgttgaaa aatgttatca attgtgtact aattaaaagt ttcgtgctta cattattttt
      121 aaaatttttg tataagtttt gacgtacaaa aactatgaaa tatacggttc aattaaattc
      181 catatttgtt gttgtttatt attttgaatt gatgaataac ttacaattgt atcgtctggg
      241 tccatatcta attgtgtctt aatattttta atatgctttt gaaccttacc ttttggaatt
      301 ttgtcttctt tagtgcatat aactaaagta ggaatatcaa aatgtttcaa ataattgtac
      361 attaagatat catcttgtgt tggatcatgt cttaaatcaa ctaattgaat aactaattgc
      421 aaattctctc tcttagttat atattcctca atcattttcc caaatttttc acgttgtgtt
      481 ttacttactt tagcatatcc ataccctgga acatccacaa aaataagttg ttcatctata
      541 ttataaaaat ttaacgtttg cgttttgccg ggttgctgtg atgtacgtgc catatttttt
      601 ctgccaatca tactattgat aaatgtagac ttacctacat tagatcgacc gctcagtgca
      661 acttcagaca attctgtttc tggatattgt tcttctttta ctgcactaat gattaattct
      721 atattattag gattaacttt cattttatat cctcactttt ttatattctc tttcaattct
      781 accattatat aatatttgaa cctttcggta tacattataa aaaagctccg atcaaagtta
      841 aactatttga gcgtcaactt tgattggagc ttttcacttt tataacacat caatgattaa
      901 gctgatgttt tactattatt aattaaattg ccttctgcgt cgtatagttc tggttcagtt
      961 tcttcattaa ttgtttgtgc tgtaataact accttcgtta cattttcgtt agaaggcaca
     1021 tcaaacataa tatcgattaa cgattcttct atgattgaac gtaaaccacg cgcacctgtt
     1081 tttctttcaa ttgctttttc actaattgct gataaagctt cttcagtgaa ctctaaatcc
     1141 acatcatcta attccagcat tttagtatat tgtttcacaa gtgcattttt aggttgcgtt
     1201 aagatgtttt tcaacgcagt tacatctaat gtttctaaat tagctacaat tggcacacgt
     1261 ccgataaatt caggaatcaa accataggct tgcaaatctt ctgggcgaat ttgtgctaat
     1321 aatgcttgtt cgtcatattt atcagcttca ttgcttgaga aaccaataac tttttcacca
     1381 agacggcgct taatcacttc ttcaatacca tcaaaggcac caccaagaat aaataagata
     1441 tttgttgtat caatttgaat catttcttgg tttggatgtt tgcgtccacc ttgtggcgga
     1501 acacttgcag tcgtaccttc taagatttta agcaatgctt gttgaacacc ttcacctgaa
     1561 acgtcacgtg ttatagatgt gttttcagat ttacgtgcaa ttttatcaat ttcatctaca
     1621 taaataatac ctttttcggc tttatcaatg tcaaagtcag ctgcttgaat taatctcaac
     1681 aagatatttt caacatcatc gcctacataa ccagcttcag ttaaacttgt cgcatctgca
     1741 attgcaaatg gtacattcaa cgtcttggct aaggtttgag ctaataatgt tttaccacta
     1801 cctgttggcc caattaatgc aatgttactt ttttgtaatt caacatcatc ttcttttggt
     1861 cctaattgtt gaatacgctt atagtggtta taaacagcta cagctaaaga ttttttagct
     1921 ttttcttgac caataacata ttcgtttaaa tgatccataa tttctttagg agtaggtaat
     1981 tctgtcatcg cttcagaagt gttttgagct aattcttctt cgacgatttc tgagcataat
     2041 tcaatacact cattacaaat atatacacca cttcctgcta caagtttttt tacttgatct
     2101 tggtcttttc cgcagaaaga gcatttcaaa ttttcttcat cttcattgaa tttaaacatt
     2161 ctttttacac ccctattcgt taaagactat actagattgg atgttacaat gcaacatatt
     2221 aacatacaaa ctttttgctt aaagaatagt agcagataca taagctatgc atctgctact
     2281 catttttgta atttaatatt taatgaagat taatcttctt tagttccttc aacgaacttt
     2341 gcgttatctc ttaataaatc gataactttt tggatacgaa catcattttt aatgatatca
     2401 gtattaccta aagtattttt gatatcttca actgagatat taaattgttt actcattttt
     2461 tctaattctt tatcgatatc ttcatcagta gcttcgattt tttcagcttc agcgatcgca
     2521 gttaaagtta agttagtttt aacacgttgt tctgcatcgt ctttcatttg ctctcttaat
     2581 tgagtttcat cttgacctga gatttggaag tacgtttgta aatctaaacc ttgttgttga
     2641 attctttgtg caaattcaga caccatacga tctaattcag tattaaccat tgcttcagga
     2701 atatcgattg ttgtattatc agtagctttt gtaatcgctt cttctttttc aacattttca
     2761 gcatctgtag ctttttgttc agctaaacgt ttacgtaagt tttctttgta ctcgtctact
     2821 gtatttgctt ctgcatctaa ttcattagca atttcatctg ttaattctgg gacttcttta
     2881 aatttaattt cgttaacttt tgttttgaaa gttgcttctt taccggctaa ttcttcagca
     2941 tggtattctt ctgggaatgt tacgacaaca tctttttctt cgtcaacttt cataccttct
     3001 aattgctctt cgaaaccagg tatgaatgaa cctgaaccga tttctaaatc gtaaccttca
     3061 gcttgtccac cttcgaattc ttctccgtca actgaaccac taaagtcgat gttaactgtg
     3121 tcgccatttt caacaacacc atcttcttta acgaccattt cagctaaatg tcctaagctg
     3181 tggtcaatcg cttcttgtaa ctcatcatca gataattcag tttcttgttt ttcaatttca
     3241 agacctttat agtctcctaa tttaacttct ggctcaactg taactgttgc ttcaaaaatg
     3301 aaatctttac ctttttcaat ttgagtaaca cttacttctg gttgtgcaac tggtttaata
     3361 tcagtttcgt caattgcttc accataagca tctggtaata aaatgtcgat agcatcttga
     3421 tataatgctt ctacaccaaa gcgttgttca aaaattggac gtggcacttt acctttacgg
     3481 aatccaggta cgttaatttg tttaaccact tttttgaatg cttgatctaa cgctttgttt
     3541 actttttctg caggaacagt aacagttaat aaaccttcgt taccttcctt tttttcccaa
     3601 gttgctgtca tgtatatata cctccatgat taactaattt attttttcaa cttccctatt
     3661 atatcatagg tctattccct atacaaacat tgaaatcaca acgtttatat atttgtaaat
     3721 caactttttt cgtcaaaact atattaaaat gctgagtatt attttgcaac aatttaacta
     3781 ttattattca aatctaattg ctgaataaaa ttaaatgcat cgatactttg taattcacat
     3841 ttaagtccta acatattttt gaaataacat tcgtatgcat taatccattt attcgtttca
     3901 aacaaagttt caatatcaat tggataaatc atgatagagt gattattcat aatatggtgt
     3961 gcttcttcag cgatatgtaa agcaccatca tttaatgttt ccataacgtt aggtataact
     4021 ttttctttaa gtgttgtatg ttctagccct tttaaattag ctggtacaaa agttacatcc
     4081 ataccatact tttcaattgt cagttcttgt gtacaatttg cgaaccttaa atactcaatc
     4141 attaaactaa tgaggttata actgtacgta ttagatttta atatatataa taccgtttct
     4201 tgaaattgaa aatgaccatt gtctattaac ttcaaaatca agtgcgtctg ttccctcatt
     4261 gataacgtat caaaatcagc taatgactga gtcaatcgtt tttcatcttc aattaatttt
     4321 gacttagcaa attctttaag aggatgtaat gccattcttg ttttgtgatc tttgacttca
     4381 tcaataattt gatgaattac ttctaccgct tcaaaatatt gtcccaaacc aatcaaactt
     4441 ttcacgtaat aaatcatcaa tgcatcatat tgttgtatgc cagttttcaa taagacaatt
     4501 gtttcttctc ttaattcaag aaatgacccc gtctcatata acatacggca tttcatcatt
     4561 gctatttctt cagtgagttc aaattgacgt tcatattgca caatatattc atacatcagg
     4621 tcatagtttt ggtcttgttc agcacgtttt atatctttat ataacttttt cgaagagtta
     4681 ggaaactgaa taatatctga catatatgtc acctcgtttg attataaatc ttgaaggatt
     4741 aatgcactcc acgtttcgaa atcatcatat gactctatgt tgcctttttc aacccatttt
     4801 attcgtaaag tatctgtttc tttagcttct acatcattcg tacttacagt gattttaaat
     4861 acaacaccaa tatgtacctt gcccacttca ttattatcgt cattaataaa accgatatat
     4921 tccatatttt gtgaatcttg ctcacttaaa cctacttctt cttctaattc tctctgtgca
     4981 ttaactctca atacttcgtt aatagattct gctcctggaa catcattcat atgaccgcct
     5041 acacctattg aagattgtcc atgcaatcga gcttctccac cgccagataa tcgttcatac
     5101 actaatatct cgccatgctc attttcaagt aaacaataag aaatgagttg tttatatgat
     5161 ggatcctctt ccatatcgcc gcgtcgctta acttcatatt gacttagcgc atcaaaaata
     5221 ttttgacctt ctggtttatt cttatttaaa aacccattga aagtattctt ttcattatta
     5281 aaaataattt ctctaggtac tacaatgatt tgttcatcaa atttagacat cgtataaaac
     5341 tcctcacttt aatgtttgtt ctcttaaatg cattttagca aatatgtcat attttagcga
     5401 ttaaatgtgg cattttgcta tttaaaaaat aaaaaagaga tgacaatgcc atctcttttt
     5461 tataagtgtt attattttaa agcatcttta gctttagtta ctaattgagc aaatgctttt
     5521 tcgtcagaaa ttgcgatttc tgataacatt ttacggttaa tgtcgatacc agcttttttc
     5581 aaaccgttca ttaaacgtga gtagctcatt tcatgttgac gagctgctgc gttgatacgt
     5641 gtaatccata atttacggaa gtcacgttta cgttgacgac ggtcacggaa agcatattga
     5701 cctgatttca ttacttgttg cttagctact ttgtataatg tatgttttga accgaagtaa
     5761 cctttagcta atttaatcgt ttttttacga cgcgctcttg ttactgttcc acctttaact
     5821 cgtggcataa aaaattcctc cttaggtaat tcctaccgat ttctgtattt gttcttattt
     5881 tttgtatgct aataattgtt ttacacgttt catatcgctc ttagacacta atctagcttt
     5941 acgtaattga cgtttttgtt tagtgctctt gtttgcgaat aagtgagatg tgaaagctct
     6001 tgaacgtttt aattgacctg aagcagttct tttaacacgt ttagctgctc cgcggtgagt
     6061 tttcattttt ggcataatat aagtcctcct gtaatagctt taattatttt tcagctgttg
     6121 gcgctaacat gataaacatt tgacgcccgt ccattttagg tttttgttca actgttgcta
     6181 tatctttgca ttcatctgca tatttttcta gcacacgttg accaatttcc ttatgcgtaa
     6241 tggcacgccc tctgaaacga atagatactt tacatttatc gcctttagtt aagaatttac
     6301 gtccgttttt caacttcgtt tggaaatcat gttcctcaat tgttggactt aaacgaattt
     6361 ctttaacatt gataattttt tgtttctttt tcatttcttt ttcttttttc tgttgttcga
     6421 atttgaattt accgtaatcc ataattcttg caactggtgg tttcgcattc ggtgcaacga
     6481 ccactaagtc taaatctaca cgttcagcca tttctaaagc ttcacgcttt gatttaacac
     6541 caatttgttc accatcttga ccgattaaac gtaattcttt tgcacgaatt ttgtcattga
     6601 tttgagtttg atcttttgct atggttgaca cctccaaaat ttttacgaaa tttgcaccaa
     6661 gcaaaaagga agagcaggta taaaataccc gctcttcctt atacacagtt atgtgtaatg
     6721 tgattaacct gccaactgct ttatgcgtcg ctacaggtga gaagcgggtg cttctacttg
     6781 gttcgtttcg tattcaacgt tattaatcat atcaacaatt cacatttaag tcaacactat
     6841 aactgtaatt atttttattt taacctttta tttcatccat tgacacgtct tgacgtaaat
     6901 ctacttgttc taatggaatt tttttcgttt tatatcgaag cttatgataa ataaagaatg
     6961 ctaaaaatac tggaattccc atatacgtaa ttaagaagcg actaaaatta aaatctcctg
     7021 ttttaataaa gtcaacatct tgaccaataa ttactacaat acataaaaag ccagcaaata
     7081 atggtccgaa tgggaataat ttagcagtat attttaattt agatttgtca taattttgtt
     7141 tatcaaatgc tcttctaaat cgataatgac ttactgctat acctacccaa gcaataaaac
     7201 cagttaaacc acttgctgca acgatatatt cgtatgcacc ttttgtaagg ctttgtaata
     7261 cgaaaattat cactacaatg attgctgtaa cgagtaacga catatatggc acaccgtttt
     7321 tatttgtttt accaaatgct tcaaacgcta atttatcttt actcattgaa tatagcattc
     7381 gagttgaagc atacatacct gagttacctg ctgataacac agacgttaaa atgactgcat
     7441 tcataaatga tgctgcaaac gcaaatccag catttttaaa cactaatgtg aatggagacg
     7501 ttgctacatt atcactaccc cccattaatg cactactatc ataaggaatt aacataccga
     7561 taacaaaaat ggctaaaatg taaaataata aaattctcca gaatacttgt ttaattgctt
     7621 tcggcacagc acgttcagga ttttctgatt caccagccgt aataccaatt aactcagtac
     7681 cttggaatga gaaaccagcg attaagaata cacctagaat tgataacaaa cttcctccta
     7741 agttgccacc aagaattgga ccttcacctt tattaaatat ttcgaatcct acaacatgac
     7801 cacccatgat tccgacaatc gttaataaac caattgcaat gaaaacaata actgtaacca
     7861 cttttatcaa tgccaaccag tattcacttt caccatagac gcgaactgat aacgaattca
     7921 gactaaaaat tataactaag aacaacgcac tccatgccca agcgggtatg ccttgcaatg
     7981 gtgtccaata ttgaatgact tgtgctgcaa tcgtaatatc tgctgctaca gtcactaacc
     8041 agttaaacca atagttccaa ccaagcgcaa accctaaaga tggatcaaca aatcttgtag
     8101 catatgtact aaatgaacct gatactggca aatacgtagc catttcgcca agtgacgtca
     8161 ttaagaaaaa taccattatt ccgataattg cgtatcctat taatgcaccc aaagcacctg
     8221 catcatgaat tgctccacca gaagttacaa ataaacctgt accaatacaa cccccaatcg
     8281 caatcataga aatatgacga tctttaagtc cccttttgac aacattgtta ctttcatttt
     8341 gaacttttga catataagct ccctttccat gttttagagg ccaagaaata aataaccttt
     8401 attcactttc ataccctttt ttacagaaca tagtgatatt aaaagtaaaa aagtggctac
     8461 atacctttaa gatatgcaac cactttggca attatctata taaggtagca catcacattc
     8521 tgtgacagta cagttcctat tcgaaaactg tcccaataga tataatttat ggtttatatc
     8581 tatttcggca tctttacctt tcacttgttc aacttatgta ccataaatac ttctgacaag
     8641 ttactaatta aacatgcaac ctctaactca atttaatatt ttaactaact tgtaatatac
     8701 aggattcatc acgcataatc aaccctgtaa aacttgatac gcaataaaag ttttaaagca
     8761 ttttattgcg acaactgtct atctatgttt tttcaaacga atttcatcaa ctagattcca
     8821 gataaattca tctttttcaa ctgtttcttg gtcttgcgat ccatattgac gcacattcac
     8881 ttgattattt tcaacttcct tatccccaac tacgatttga taaggtattt tttgcatttg
     8941 agcttctctg attttataac ccattttttc attacggtca tcaatactta cacgaacgcc
     9001 ttgagatttc aattcatctt gtaattggcg cgcataatca taatgtaaat caacgttaac
     9061 tggaatgatt tgaacttgtt ttggcgctaa ccaagttggg aatgcacctt tagtttcttc
     9121 agttaaaaat gctacaaatc gttccattgt tgatacaaca ccacgatgaa taacaactgg
     9181 acgatgatgt tcaccatctt gaccaatata agttaaatca aaacgttctg gtaataagaa
     9241 atcaagttgt gctgttgata atgtctcttc tttacccatc gctgttttaa cttgaacatc
     9301 tagtttcgga ccatagaatg ccgcttcacc aatcgcttct tcgtacgata agccaagctc
     9361 atccgctgcc tctttaagca tattttcagc tttattccac atatcatcat catcaaagta
     9421 cttttcttta tcttcagggt ctctataact taatctaaag ctataatcct cgaaaccaaa
     9481 gtctttatac acatcaataa tcatgtttac aacgcgtttg aattcttctt taatttgatc
     9541 aggtcgaaca aagatatgtg aatcatttaa agtcatacca cgaacacgtt gtaatcctga
     9601 tacagcacca cttgcttcat atctatgcat cgttcctagc tcagcgatac ggataggtaa
     9661 ttcacgatat gaatgtggtt tattcgcata aatcatcata tgatgtggac agttcattgg
     9721 acgtaatacc atagattcag tttcatctaa ctgcattggt gggaacatat cttcttgata
     9781 gtgatcccag tgaccagatg ttttgtataa atcaacatta gcaagtactg gtgtataaac
     9841 gtggtcatat cccatgctaa cttctttatc aacaatgtaa cgttcaattt cacgtctaat
     9901 tgttgcaccg ttaggtaacc ataatggcaa accagcacca actaattggc tatttgtgaa
     9961 tagttctaac tctttaccaa ttttacgatg atcacgttct ttacgctctt ctaacatttg
    10021 taaatgtgct ttcaattctt ttttatcaaa gaaagcagta ccgtatatac gttgtaacat
    10081 tttgttgtta ctatctccac gccagtatgc acctgctgta gataatagtt taaactcttt
    10141 aattttagct gttgatggaa cgtgaactcc acgacataaa tcagtaaaat caccttgact
    10201 atataatgtt acattttcat cttcaggaat cgcgtcgatt aattctaatt tgtattcatc
    10261 attgctgaat aactctttcg cttcatctcg tgaaaccact tttcgttcga ttttcatatt
    10321 ttcgttaacg atttgtttca ttgttttttc aatttgttca aagtcatcag atgagatgtt
    10381 ttggtcaatg tcgaagtcat agtagaatcc accttctatt acaggaccta caccaaattt
    10441 aacattacca tataaccttt taatcgcgtg tgccattaaa tgtgcagtag aatgacgtaa
    10501 tacctctaac gcttcttcac tacctggtgt cacaatttca attgatccat cagtttcaag
    10561 cggtttagtt aaatctacaa gttgcccgtt aaatttgccg gcaacagctt ttttacgtaa
    10621 tccaggacta attgattgtg ctatatcttc agtagtagta cctttatcaa acgccttttt
    10681 attaccatct ggaaattgaa tattaatttg ttccatgtta aaccctccta tttgctttat
    10741 aaatactttt atctgacctc gttaaaaaat tgaacaacaa tcatcgtttt aacattaaac
    10801 ctattaacaa taaacaggga atttatgaca ccaacaaaca tttatgtctg aaaaaaacaa
    10861 aaaatcccgc ccctatacaa gggacgagat cgtcgtggta ccaccctagt tatctaatac
    10921 aatttatgta ttaaatatct ctgcttaaga taacggtctt gatccgggta ttcattacaa
    10981 ataccataaa tgaagtagta atcatctaat ttattaacca tattctcatc aaacaatggc
    11041 tttctgtgta atattgatta gatcatcttg tcttcaatca tttaaacgat ttagatttgt
    11101 attcattata caccaatcat tttaaaattc aattgtttct gaaattttct cctgataaaa
    11161 agtatggtgt tgacaaagat ttgacacgtt caataatacg tgctgcttta gtcttctctt
    11221 caccatcacg agtcatcgct aaatgatgtt ccaattcact atagtcaaaa ttagaactaa
    11281 agaatgttgg taattcatga accattcgat aatgtagcaa aggtccaatt acctcatctc
    11341 tcacccatgg agtcacttct tcagccccaa tatcatcaag cattaaaatg tttgcttctc
    11401 ttacgcgatg taatttcttt tcaaaagaac catctttaaa gccacctttt aatgttctaa
    11461 taaattccgg taaataaata attgtcgaac gtaccttctt agatttgagc tgattcgcaa
    11521 ttgcacctag aataaaagat ttacctgtcc caaatggacc ataaaggtaa aggcctttca
    11581 cttgttcccc attagttatt gctgtacaaa tatcatctgc tgccatagct acatcaagac
    11641 ggtctcgatg attcatataa atatctttca atttggcatt taaagtatct cgttgcatat
    11701 gatgagatgt aattagctca gcttcaaagc gttcttcgtc gtacttgatt ttacatgggc
    11761 attgtaaata gcgtatttta attcggttat tatcaacata taactcaggc acatgcccct
    11821 ttacgaaatt tggacaatca gcaaatttat gaccgtcata atgtttttgt tgatctttat
    11881 actcttgtaa cacatttaag tcttcatcaa tcatagcatt cgttaattca gctcgatgcg
    11941 cttccaaaaa ttgcttaaca tctgggtcat tgattacttc tttttttatc ttttctattc
    12001 ttttttcaaa gtcctgcgac gtgttaatta tacttttaaa ttgcttcatt attgactgtc
    12061 ctcctcccat tttttagata atttatctag aaatgcttgt cgatcttgct ctaattgttg
    12121 atcatctacg ctattatctt tagccgaatc ttcttcacta ggtttatctc tattttctaa
    12181 ccatttaggt gttttttctt ttgaaatacg attacgctgc ccatagtatg aaccacgctt
    12241 ttggtaattt ccgctagaac cctcattttt aggttgatta acttttttag cgtaattata
    12301 tgcttcttta gctgtcttaa tacctttttt cttccaattt gatgctattt ccaaaatata
    12361 cgctttagga agtttcatat cttcttttaa catgacaaat tgcaacaaaa tattaatgac
    12421 gccaaaagac attttttcac gttcaattaa ttcttcaacc attgtctttt gcgatatagt
    12481 tggttctgat tcagaccaag aagctaacat atcaattgga ctcgtttgtt caagtaactc
    12541 aaaccattca tcactttgtg gctttggatt cacttctgaa gatttgcccg ccgaagatga
    12601 tgtagcagga gatttcacct gtaatttagg catttgattt tcgtgttcca ttaagtaata
    12661 cgagcgtgct tgtttacgca tttcttcaaa ggataactgt tgtccacttg taattgaatt
    12721 taaaataaca tgcttcatgc catctgctgt taaaccatat aaagtcgcga gttgtgtaat
    12781 taaacgcttt gcatctttgg taacaatgtc ttgactaata aaatgtttac ctaacatttg
    12841 tctcaacatt tcaaagtcaa aagattcatt tgataaatcg ataccttggt acggttcatt
    12901 aatcggaata tcacttgtat cgacatctat ttttgtagac ggcactttaa aaacatcagt
    12961 aaattgtctt gttacctgtt taaattcact caaatcaatt tgttgatact caaagtattt
    13021 cttcaactca tgaaatcgac gatgctcgac ttcactatat aaaaagattg acaacattgg
    13081 atcattaaaa aataaatgtg ctgaaggcgg ttgaattaat tggtaaacaa attgtgtttc
    13141 ttgttcatca tgtttgacaa acgcctttaa caatccaatc gcttcaagta agtccatttg
    13201 ttgtctaaac tctagtaaat taattttaag ttcattcata aaaatataat gagaaagaat
    13261 caatgtttca ttatgacttt ctttaacgaa ttgagtcata aaatgatata aacccactgc
    13321 ttgcgttcca attagcggtg tatacagtcg attcaatacc tctaaatgat tcgtatttaa
    13381 atcaaagtgt tgcataactt tgaattgatc ctttggtctt aagccgaatt cgaaggcttg
    13441 tcgtcccatt taagcatcac tccgtttgtt ttcgcttaaa atcccttgca tcgatgctaa
    13501 caattgatca acatctttaa attctttata gactgatgca aatctaacat atgaaacttg
    13561 atcaacatgc attaacaagt tcataacgtg ttcacctata tctcgtgaag acacttccgt
    13621 atgaccttca tctcgtaatt gccattcaac cttgttagtt atgtcttcaa gttgttgata
    13681 tctaactggt cgtttctcac aagaacgcac aagtccatta agtatctttt ctcttgaaaa
    13741 ctgctctctt gtgccatctt ttttcacaac tataagctga ctaacttcga tatgttcaaa
    13801 tgtagtgaaa cgtgttccac aattttcaca ttctcttcgt cttcgaatgg catttaattc
    13861 atcggcatgc cttgaatcta caactttaga ttgtgtagaa ttacatttcg ggcatttcat
    13921 tacatcaccc tctttatttt gattatgcct aattatacta taaatctaga gatgaaaaaa
    13981 gaatccctca atttaattca tttaaccaaa taatgaaaca ataaaaaaca ttatatcgtt
    14041 acttattaag taatttgcat gacaatatta ttgtattaaa aataaaaaac ctaactccga
    14101 agtcagagtt aggctataaa ttaattgtat taacttgcac ttacagtttc ttttgatgtc
    14161 aaaagtgctc caatttgctc agcaacatct acaactctat ttgaataacc ccattcatta
    14221 tcataccaag caataacttt tactttattc cctgacatga ccattgttga ttttgcatca
    14281 ataatagctg aatttggatt agtattaaaa tcaacagaca ctagtggttg atgttcgact
    14341 tctatgatac cttctaaacc tgcattttca aaagcttggt ttacttcttc tgcagttact
    14401 tctttttcta aatcaacaac taaatcaacg agcgatacat tctttgttgg tacacgtaat
    14461 gccatgccgt gtaatttacc ttctaattct ggtaatactt cttttaaagc tttcgccgca
    14521 ccagtagaag taggaataat gctttcatta catgaacgtg cacgtcttaa atctttatgt
    14581 ggattatcaa tatttttttg gtcatttgta atagcgtgaa cagtagtcat taaaccatta
    14641 actattccaa actgattatt taaaactttt gcaactggac caatgcaatt agtagtacat
    14701 gaagcattac taaaaatgtc aaatgcttct atatctaatt ggttatcatt tacgccttta
    14761 actaccattt gaacatgtcc accttttgaa ggaccagtta acaaaacttt tttggcacct
    14821 gctttaatat gtgcgatggc tttatcacca tgattaaatt taccagttgc atctatagca
    14881 atatcgatat ctaattcttt ccatggcaag ttttcaggat tgcgatcagc aaccaattta
    14941 attttatgat ctccaacttg caatccattt tcaatcggtt caacttttag attatatttt
    15001 ccatgtgtcg tatcgtaatt gattaaatgt gcaattgttt cgggtggata actagcattt
    15061 atcgctacta catttaaatt tttattttgt aatgcaatac gtaataccat tcttccaatt
    15121 ctacccatac cattaattgc aatattcgtt gacattaaaa gcaccccttg atctttatgt
    15181 gttatacttt atggaattag tatagcatat tatatgaaat ttgaaagcgt tttccgttga
    15241 tttatttatc ttttttataa aaaatagtca ttacttacat acatcaacgc gtacgtaaag
    15301 taatgactta tttatagtgt aatattaatc ttcttctccg taattcggct tttcaatata
    15361 accttcttct tctaacaatc tctcaaggtt ttgttttaat tcaagtttat cccctaaatt
    15421 atcgataaca tgatcggcca ttcggctttt tttatcaata gaaatttggc tatagacacg
    15481 tgctttcgca tcttctaatg acaaattatt acgttgcatt aaacgatcca tttgtatact
    15541 ttcagaagtg tatacaaccc acacttcgtc tactgtattt tccaattcat tttcaaataa
    15601 taatggaata tccatgatta cattatatcc ttgttttaaa tattcttgct tttcttcttc
    15661 cataatatct cgcacgatag gatgtatgat agcatttaat tctaagcgtt tttctggatg
    15721 attaaacact agatcaccca tataacgacg attcatctca ccattttcat caattgcttc
    15781 atcaccaaag acttctcgta cttgagctaa acctttactc ccctttttaa cagcttccct
    15841 ggctgcttta tcagcatcta ctactttaaa accgaatacg gataagagtt ctgatactgt
    15901 tgattttcct gaggcgattc cacctgttag accaataact ttcggcataa tttcactctt
    15961 tctttatttt tgacatactg gacaataatg actatttctt gtcgcgatga tttttgtttc
    16021 aatttgactt ccacacactt tgcataccgg ctgcttatat acattaagat gcaattgcat
    16081 ctcaccagtt tttccatcag catgacgata atctgaaata cttgtaccgc catatttaat
    16141 accttcttct agtacttctc taacataata aaaaaccatt tcttgttgtt ggtgtgttaa
    16201 gtcttttact tttttatctg gtaaaacacc tgcacgaaac aacgcttcac atgcgtaaat
    16261 atttccacaa cctgcgatta ctttatgatc caaaatcact tgtttgattg gtttattctt
    16321 attagactgt tgatgaattc gatttaaata atacgtcaat gcttcatttg aaaaaggttc
    16381 aggcgctatt tctaaaaatg aaggataaga tgctacagac gcaacatttc taatttctcc
    16441 aaaacgacgt atatctgaat aaattaactt tttgtcattt gacaactcaa aaataacatg
    16501 ccaatgctta cgataattag gtatcataat atcttcaagt tcatctacaa tgaaaaaacc
    16561 gcccgccata cctaaatgac taattaatgt acgttgttct cgtttattat ctagctgaaa
    16621 aacgatatat ttacttcttc gttctacatt tgtaatggta tagccttccg ataaagtttt
    16681 aaaagtatct aattcaattc cttttataat tgtttccttg ccttgagctt taccttcgat
    16741 tactttatcc gaaaatataa cgtgttcaat tttttgattt ataacgtagg gttcaattcc
    16801 tctttttaca tgttctactt ctggtaattc gggcatacca ttaacctcac tttattttgc
    16861 atcataccag gttgcaccat aacttgagtc tacttttaat ggaacatcta attgcaatgc
    16921 attttccatt atctcttcta caaattcact aaatgaatct acttctgact taggtacttc
    16981 aaaaattaat tcatcgtgta cttgtaataa tagtttagct tgatatgttg tctctttcat
    17041 tttttgagca aatttaacca ttgccagttt aatgatatct gcagcactgc cctgtattgg
    17101 cgtattcata gcagtacgtt cagcaaagcc gcgtaaatta aagttacgac tcgtaatatc
    17161 aggaatatag cgtcgacgat gtagcaatgt ttccacgtaa cctaaagctt tggcatcttt
    17221 tacaatatca gacatatatt gttttacacc tgggaaacta gctaaataat catcaatgaa
    17281 tgcttttgct tttttacgag taatacctaa actttgactt aaaccataat cacttatccc
    17341 ataaacaatt ccaaagttaa ctgcttttgc ttgacgacgc attaaactat cgacttgatc
    17401 agcttctaca ccaaatactt tcatagcagt tgctgtatga atatcatcgc cgttgataaa
    17461 tgcttctttc atactctcat cttgtgtaat gtgtgctaat acacgcaatt caatttgaga
    17521 ataatctgct gataatataa cgctatcttt tgaagttggt ttaaaggctt ttcttatttt
    17581 acgcccttct tcaagtctaa ccggaatatt ttgtaaatta ggatctacac ttgataaacg
    17641 tccagtttgc gctaaagttt gattaaaacg tgtatggata cgttgatcat cactaattac
    17701 cttttgcaat ccttcaacat aagtagactg taacttagat aattgacgat attctaaaat
    17761 ataatcaatg ataggatgtt caccttgcaa ttgctctaag acatctacag cagtagaata
    17821 tcctgttttc gtctttttaa taacaggtaa ttgtaatgtc tcaaacaata caacacctaa
    17881 ttgcttagga gaatttatat taaaatcttc accagctgca tcatggatat ttcgaatcaa
    17941 gacgtctaat ttttcttgaa tttctttttc catttcttct aaatcatgaa catctgtaaa
    18001 tataccaatt tcttccattt cacttaaaat cttagctagc ggtagctcta aatcagccaa
    18061 gagttctacc tgattgtatt cttctaattg tttatccata tttggtttcg caaagtaaat
    18121 tgcatcagta atagaagcaa catatggatt taaaacatca tcttcaggta ccttaaattt
    18181 cttacctttt ccatatatac tcacatcgtc tttcacaaaa ctttgaccgt acaatgaaac
    18241 aactgattga acatcactaa tcgtacgaga tggatcaatt atataactgg ccaacataat
    18301 atcgaaagaa atattttgaa tatcaatccc caatctatgt gatgctacat atgttttttt
    18361 agcatcatat acgacttttt tcgaattcgg attttctaac catgaaacta gttcgacata
    18421 attatttatg tcatccgcat taattacaat atgtttctca cctgtaaata aagagaattt
    18481 taaaatatta tttcgcaaat agttaccacc gtctaattcg aaatggatgg ccgcttcttt
    18541 caatgaagta aaatcaatat tatcaaaaga cgtttcaatt tcaaatgtct tttctattgc
    18601 atcttcaacg cttgctgatt gatcaatgtc agccaacaat tgtttgaatt ctaacttctt
    18661 aaacaattcg attttttctt gttgttcatc ttgatgagtc attaacgtat cttcaagttt
    18721 tacttcaatc ggactatcta cattaatcgt tgctaattct ttactcatta atgcatcttc
    18781 tttgctattt tgaagttttt cttttaactt tttacctgaa atttcatcta aatgttcata
    18841 gacaccttct actgtgtcaa attggtttag caattttatt gctgttttct ctccaacacc
    18901 tgcaacacct ggtatattat cagaagtatc tcccattaat cctttcatat caataatttg
    18961 attaggtgtt aacccgttgt atttttccgc aataaagtca ggtgtataat gatcaacatc
    19021 agtaacacct tttttagtgt aataaatggt tacattatcc gttgcaagtt gtgttaaatc
    19081 tcggtctccc gtaataataa ttgtctgaaa gcccgcttta tctgcttctt tacttaaagt
    19141 tccgataata tcatctgcct catagttatc taattcataa cgtttaatat gataagcatc
    19201 taataattgg cgaatataag gaaattgctc acttagttca ggcggcgttt tctggcgtcc
    19261 acctttatat tcactatatt tttcatgtct gaaagtcgtt ttacctgcat caaacgctac
    19321 taaaaaatga tttggctttt cttcttttaa aatcttctct agtaacattg caaaaccata
    19381 tactgcattg gtatgaatgc ctgctttgtt tgataacaaa ggtaatgcat aaaaagctct
    19441 aaaacttaag ctattaccat cgattaatac taatttattc acaattttaa cctccagaac
    19501 taatttatat atattttagc atattcgact gtgatgtcgt aattaaagaa actatctcat
    19561 agagtattta tacacgacct aatttcaaat aaaacaagtg cctatataga cttagctaaa
    19621 aaataaaaaa gacataatca tgattgttct tgatgagcac tccataaagt caggttaaaa
    19681 gtccaacttt tgagatgcac attatcattt catgattatg tctacttttt aatattttta
    19741 tgctgctatt ttaaattttt ataggtaaga ataagttatg aatcgtctga accaaatgga
    19801 atatatttaa ctttggtcat tttcggtaat tcttcataat tattaaacca ctcttggtaa
    19861 tttttattga ttggtg
//
LOCUS       DAKTIO010000032        19667 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.032, whole genome shotgun sequence.
ACCESSION   DAKTIO010000032 DAKTIO010000000
VERSION     DAKTIO010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19667
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.032"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..219)
                     /locus_tag="O0293_000870"
     CDS             complement(<1..219)
                     /locus_tag="O0293_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCY0279764.1"
                     /translation="MKRQAKIEIQNALVDLMAEYPFQEISTKMICAYCNINRSTFYDY
                     YKDKFDLLDTINSKHKEKFQFLLSALHHN"
     gene            340..987
                     /locus_tag="O0293_000871"
     CDS             340..987
                     /locus_tag="O0293_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCY0279765.1"
                     /translation="MKKMILINVITVVVLLAIGIAGFYFWNKTTSYVTTDNAKVNGDQ
                     IKIASPASGQIKSLNVKQGDKLDKGDKVAIVTVQGQDGETKDMDLKMPQKGTIAKLDG
                     MEGSMVQAGNPIAYAYNLDDLYVTANIDEKDIKDVEVGKDVDVTIDGQKASIKGKVDS
                     IGKATAASFSLMPSSNSDGNYTKVSQVIPVKITLESEPSKQVVPGMNAEVKIHKN"
     gene            1000..2931
                     /locus_tag="O0293_000872"
     CDS             1000..2931
                     /locus_tag="O0293_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCY0279766.1"
                     /translation="MTTTFIISYIILALIIVGVINLFLIRSRKKGKRQQKEQQFTTRQ
                     SNQSKFKASDLDKTTDQSTQRMTHEELRVDNQDDHSQVSLNGYTKGSEKDQEAFTNNK
                     DEEAVAAKNPESEEYKVNEKIKKEHKNFIFGEGVSRGKILAALLFGMFIAILNQTLLN
                     VALPKINTEFNISASTGQWLMTGFMLVNGILIPITAYLFNKYSYRKLFLVALVLFTIG
                     SLICAISMNFPIMMVGRVLQAIGAGVLMPLGSIVIITIYPPEKRGAAMGTMGIAMILA
                     PAIGPTLSGYIVQNYHWNVMFYGMFIIGIIAILIGFVWFKLYQYTTNPKADIPGIIFS
                     TIGFGALLYGFSEAGNKGWGSVEIETMFAIGIIFIILFVIRELRMKSPMLNLEVLKFP
                     TFTLTTIINMVVMLSLYGGMILLPIYLQNLRGFSALDSGLLLLPGSLIMGLLGPFAGK
                     LLDTIGLKPLAIFGIAVMTYATWELTKLNMDTPYMTIMGIYVLRSFGMAFIMMPMVTA
                     AINALPGRLASHGNAFLNTMRQLAGSIGTAILVTVMTTQTTQHLSAFGEELDKTNPVV
                     QDHMHELASQYGGQEGAMKVLLQFVNKLATVEGINDAFIVATIFSIIALILCLFLQSN
                     KKAKATAQKLDADNSINHE"
     gene            complement(3177..3815)
                     /locus_tag="O0293_000873"
     CDS             complement(3177..3815)
                     /locus_tag="O0293_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279767.1"
                     /translation="MTNPNQRLEPFDETFQQPNIHRGKRYGKKKRSLVSMIIQIIVVI
                     LTAIAGYSMWKQPIFNIVFTKESVDFDGIKNFNETIGKMHQLNINLGNINDLQQGIDR
                     LILVFNGFFILCIVSLVITILTIVFNRTVLKVVNILVLTIMLVITLYFSYIIKALAQE
                     IADVFKKYYLNVPPDQVITEADAIHNGIILLGCSIGLLVISLFFRNRMPRLK"
     gene            4023..4943
                     /locus_tag="O0293_000874"
     CDS             4023..4943
                     /locus_tag="O0293_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0279768.1"
                     /translation="MIRNRVMNSVVNKYLLHNRSIMFKNDQDVERFFYKREIENRKKH
                     KQPSTLNVKANLEKLSLDDMQVFRFNFRHQIDKKILYIHGGFNALQPSPFHWRLLDKI
                     TLSTLYEVVLPIYPKTPEFHIDDTFQAIQRVYDQLVSEVGHQNVVVMGDGSGGALALS
                     FVQSLLDNQQPLPNKLYLISPILDATLSNKDISDALIEQDAVLSQFGVNEIMKKWANG
                     LPLTDKRISPINGTIEGLPPVYMFGGGREMTHPDMKLFEQMMLQHHQYIEFYDYPKMV
                     HDFPIYPIRQSHKAIKQIAKSIDEDVTQNN"
     gene            complement(5051..5470)
                     /locus_tag="O0293_000875"
     CDS             complement(5051..5470)
                     /locus_tag="O0293_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279769.1"
                     /translation="MTDALQQKIHIELLDLLDDVKFELTELNAQKGLYINGPANQLLK
                     RGVHMAYVQGQKQAIDNIMTIVEQQLEDQHFLEHYDKFQNEVAHRNYDKTANFAELSD
                     IPRQFDNFLDQFYQIKGQYFIITHINTLIGDFHSEAH"
     gene            complement(5467..5958)
                     /locus_tag="O0293_000876"
     CDS             complement(5467..5958)
                     /locus_tag="O0293_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCY0279770.1"
                     /translation="MVIIKQLFTHTQTVTSEFIDHNNHMHDANYNIIFSDVVNRFNYS
                     HGLSLKERENLAYTLFTLEEHTTYLSELSLGDVFTVTLYIYDYDYKRLHLFLTLTKED
                     GTLASTNEVMMMGINQHTRRSDAFPESFSTQIAHYYKNQSTITWPEQLGHKIAIPHKG
                     ALK"
     gene            6055..7002
                     /gene="corA"
                     /locus_tag="O0293_000877"
     CDS             6055..7002
                     /gene="corA"
                     /locus_tag="O0293_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCY0279771.1"
                     /translation="MALKIRYQTTYEPFKVVDDIKEIPKDATIVWYDFDEPNEQENEW
                     FKAHFNFNDLEVDDAINGMPRAKYKSYKDYQYLVFHSIMGSNYSPIALNIFIQDNVLV
                     TYHHQTLESLNKVVYKYMNTLDAELDCADVVILILDMMVDKYFNFVYALEDSVYHFED
                     RHVDDRFNKMVMDSVFKLRSDLIKVKRVLFPMQELIDTMKQNGDLIIDNKHSLYIQHI
                     DDHLIKQRNIIRTAQEMTNEIRENFESYTSFRMNSIMQVLTLVSVIFSPLTFIAGIYG
                     MNFVNMPALHLHYGYYICLAVMFVIAVVLIIFFRRKKWF"
     gene            7033..8082
                     /locus_tag="O0293_000878"
     CDS             7033..8082
                     /locus_tag="O0293_000878"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCY0279772.1"
                     /translation="MSDFQREQRKNEHVEIAMAQSDAMHSDFDKMRFVHHSIPSINVN
                     DIDLTSQTPDLTMAYPVYINAMTGGSEWTKNINEKLAVVARETGLAMAVGSTHAALRN
                     PRMAETFTIARKMNPEGMIFSNVGADVPVEKALEAVELLEAQALQIHVNSPQELVMPE
                     GNREFVTWLDNIASIVSRVSVPVIIKEVGFGMSKELMHDLQQIGVKYVDVSGKGGTNF
                     VDIENERRANKDMDYLSSWGQSTVESLLETTAYQSEISVFASGGLRTPLDAIKSLALG
                     AKATGMSRPFLNQVENNGIAHTVAYVESFIEHMKSIMTMLDAKNIDDLTQKQIVFSPE
                     ILSWIEQRNLNIHRG"
     gene            complement(8130..9338)
                     /gene="gltS"
                     /locus_tag="O0293_000879"
     CDS             complement(8130..9338)
                     /gene="gltS"
                     /locus_tag="O0293_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCY0279773.1"
                     /translation="MIELNAITTLCLACILYLLGKAIVNHVNFLKRICIPAPVIGGLI
                     FAILVAALDSFGMVKIKLDASFIQDFFMLAFFTTIGLGASLKLFKLGGKVLLLYFMFC
                     AIISVIQNIVGVSLAKVLNIKPLLGLTAGSMSMEGGHGNAAAYGKTIQDLGIDSALTA
                     ALAAATLGLVFGGLIGGPVVKFLIKRYNLKPQHSDDTFKDYSQVAYNEHLHSKFNATE
                     VFFIQFTIVVFCMAVGSYFSHLFTAQTGINVPIYVGSLFVAVIVRNISESFNFNIVDL
                     KITNQIGDVALGIFLSLALMSIQLIEIYKLAIPLIIIVLVQVVVMILFAVLILFRGLG
                     KDYDAAVMVGGFIGHGLGATPNAMANLDVITKKYGNSPKAYLVVPIVGAFLIDLIGVI
                     VIMGFIQWFS"
     gene            complement(9675..10283)
                     /locus_tag="O0293_000880"
     CDS             complement(9675..10283)
                     /locus_tag="O0293_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HCY0279774.1"
                     /translation="MDFVNNDTRQIAKNLLGVKVIYQDTTQTYTGYIVETEAYLGLND
                     RAAHGYGGKITPKVTSLYKRGGTIYAHVMHTHLLINFVTKSEGIPEGVLIRAIEPEEG
                     LSAMFRNRGKKGYEVTNGPGKWTKAFNIPRAIDGATLNDCRLSIDTKNRKYPKDIIAS
                     PRIGIPNKGDWTHKSLRYTVKGNPFVSRMRKSDCMFPEDTWK"
     gene            10682..11329
                     /locus_tag="O0293_000881"
     CDS             10682..11329
                     /locus_tag="O0293_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCY0279775.1"
                     /translation="MEQLPQVGFMKSFVLFWKNYVNFKGRSRRSEYWYMALWHLILMV
                     PAVCVLIINLFLFFISIAADNETLIVMSILMIILTSVYTFVYSLATFIPNLALCVRRF
                     HDISRTMLFPMIMTVFSIIFYIVLQIIDSYYDSDFTLMPMGLNILLVLLYFVYLGLTI
                     VMIVFLCFDSKPANKYGESPKYPSTIKNQPVTSETPEKACTSETPKTPEETDNQL"
     gene            11560..12459
                     /locus_tag="O0293_000882"
     CDS             11560..12459
                     /locus_tag="O0293_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0279776.1"
                     /translation="MGLVIEHVTKRFGKMTAVNDISLKLESGKMLGFLGRNGAGKTTT
                     FRMILGLSEPTEGHITYNGKKLDKTMYNRIGYLPEERGLHGKLTVEEELKYLATLKGM
                     SKTEIQQQISYWLERFDITENRKKRIDSLSKGNQQKIQLLASMLHKPELLILDEPFSG
                     LDPVNVELLKEAVKDLNDWSSTIVYSSHRMEHVEELCDDVCILDKGQLVVSGDINHVR
                     ASNGNKKVVIESETTLPDLTNIRGIIHSENMKQGLQLTIENEDVAKDIYQVVAHQGYV
                     KRFQVVEPSLQDIFIEKVGGKDA"
     gene            12452..13684
                     /locus_tag="O0293_000883"
     CDS             12452..13684
                     /locus_tag="O0293_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0279777.1"
                     /translation="MHKFWATFTLTYIKKIKAKSFVIFMIIIAALMIGLSNIDKIINM
                     FDDGPDKIGVAAPNEQIYKVFKQQANTFHSDAKFTKVSIEDAEKEVKKHKLDKAYIIK
                     VNQNRTLQGTIISEKRVSHEDSQKVQALLTAIQTNMVAGELNINKEDLQKLQAQSKVD
                     NKVISNDEVDKVSEGQKIFNYALAYGIIFLMFFIVLNYASQIAMEIASEKTSRVIEMI
                     ITSISPIQHIFAKILGVIAVAVTQIILIVLMAVICIFAFDLKDLLQGFNVEMNQLSWQ
                     IIIVGIISSIVGITAYVLLAAILGSLTSRIEDLNQSLMPLTLLGMIAFYIAIFTINNP
                     DGQLALITSYIPFLAPFQLVVRAQTSGLQIYEVILSSLISIVMVAVLIWIAIKTYKDS
                     VLTFERGLFNSMKRVFKK"
     gene            13668..13853
                     /locus_tag="O0293_000884"
     CDS             13668..13853
                     /locus_tag="O0293_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279778.1"
                     /translation="MYLKNKIDESDTHLSYKMMMKACYSYFFIFSYRIMSKKVYNMNV
                     KTNFLKWECEVRRNVNK"
     gene            13902..14228
                     /locus_tag="O0293_000885"
     CDS             13902..14228
                     /locus_tag="O0293_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCY0279779.1"
                     /translation="MLYPIFIFILAGLCEIGGGYLIWLWLREGQSSLVGLIGGAILML
                     YGVIATFQSFPSFGRVYAAYGGVFIIMSLIFAMVVDKQMPDKYDVIGAIICIVGVLVM
                     LLPSRA"
     gene            14258..15277
                     /locus_tag="O0293_000886"
     CDS             14258..15277
                     /locus_tag="O0293_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCY0279780.1"
                     /translation="MIVEVEHQRQGVDLIKIDNDETKIIFTNYGARVVSWKYHDNNIV
                     LGNVVEADEFYHSNPFKFGASIGRYSGRIDNAKFKLKGKEYQLEKNNGEHHLHGGCHG
                     LDNKLFDYEIRNEIAQIKVIFKTVLKSADDHFPGDIDVTITHIYDADHQWSIEYEAVA
                     SEDTLFSPTNHVYFNLNRDNNVVDNHRISSNQLDMYVLDERNIVTGDILDLHEVFEDN
                     KIKLSDIFTSQHAQLSQQMTRFGGLDHPFTVGEHKMYVENHEFMLEVDTDMPHVVFFT
                     FNQPDEWDSPFNIYKPHSGFTLETQFLPNDINLYGEKAPSILKAGEVFNSKTSFRIEE
                     KVEND"
     gene            complement(15513..16166)
                     /locus_tag="O0293_000887"
     CDS             complement(15513..16166)
                     /locus_tag="O0293_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HCY0279781.1"
                     /translation="MISIHAISTGKIQDLPYSSKRPMRSALDKTKISQTTWLSSTGFT
                     GDEQAYKDHGGPHKAVCGFSKHNYALYQDDLPTLPTHAMFGENLTFDYLDESDVYFGN
                     QYRLGEALIEVSEIREPCWKIQAKYNIPDLVKRMSTSGKTGFYFRVLKQGYVSPNDQL
                     YLIQEAPIEHRLSVQQLNDLYYNDRQNQDMLRYALNNPFLSPTRRDKLQKMYNRTLK"
     gene            16340..17026
                     /locus_tag="O0293_000888"
     CDS             16340..17026
                     /locus_tag="O0293_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HCY0279782.1"
                     /translation="MKDVKALKLMTLNDVLSQINGDMTLGIGTGSTMELLLPQMAQLI
                     KERGYNITGVCTSNKIAFLAKELGIKICEINDVDHIDLAIDGADEVDPSLNIIKGGGG
                     ALFREKVIDEMASRFVVVVDETKIVQYLGETFKLPVEVDKFNWYHILRKIESYADIKV
                     ERRVNEDVAFITDNGNYILDCKLPKGIDPYKFHEYLIHLTGVFETGYFLDMADQVIVG
                     TQEGVKILEK"
     gene            complement(17092..18351)
                     /gene="sdpC"
                     /locus_tag="O0293_000889"
     CDS             complement(17092..18351)
                     /gene="sdpC"
                     /locus_tag="O0293_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpC"
                     /protein_id="HCY0279783.1"
                     /translation="MKNNKISGFQWAMTIFVFFVITMALSIMLRDFQSIIGVKHFIFE
                     VTDLAPLIAAIICILVFKYKKVQLAGLKFSISLKVIERLLLALILPLIILIIGMYSFN
                     TFADSFILLQSTGLSVPITHILIGHILMAFVVEFGFRSYLQNIVETKMNTFFASIVVG
                     LMYSVFSANTTYGTEFAAYNFLYTFSFSMILGELIRATKGRTIYIATTFHASMTFGLI
                     FLFSEEIGDLFSIKVIAISTAIVAVGYIGLSLIIRGIAYLTTRRNLEELEPNNYLDHV
                     NDDEETNHTEAEKSSSNIKDAEKTGVATASTVGVAKNDTENTVADEPSIHEGTEKTEP
                     QHHIGNQTESNHDEDHDITSESVESAESVKQAPQSDDLTNDSNEDEIEQSLKEPATYK
                     EDRRSSVVIDAEKHIEKTEEQSSDKNK"
     gene            18641..19576
                     /locus_tag="O0293_000890"
     CDS             18641..19576
                     /locus_tag="O0293_000890"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HCY0279784.1"
                     /translation="MYKQGEPNLWTGRLDSETDPKKFRHFQTVTFEDLSKLEKSSMPS
                     GVGILGYAVDKGVALNKGRIGAKEGPDAIKQAFAGLPDLNQCETLVDYGNVYHDHEEL
                     IDTQKEFAMLAAKSIANHRQTFLLGGGHDIAYAQYLATRKVYPTQSIGVINIDAHFDT
                     RAEQQSTSGTSFRQILEEDENTDYLVLGIAQGGNTQSLFDYAKEKKIDYVFADELLSH
                     VSPTIKDMIERFVHEHDVIMFTICMDVIDSAFAPGVSAPAVLGLYPHTVLELAKRIIP
                     SDKVSSVSIAEMNPTYDADNRTAKLVANLVHHFLK"
BASE COUNT         6845 a         2792 c         3552 g         6478 t
ORIGIN      
        1 attatgatgt agcgcgctta gaagaaattg gaatttttcc ttatgctttg agttgatggt
       61 atccaataaa tcaaatttat ctttgtaata atcataaaaa gtagaacgat taatattaca
      121 atacgcgcaa atcattttag tggatatttc ttgaaaagga tattcagcca tcaaatcaac
      181 aagtgcgttt tgaatttcta ttttggcttg tcgtttcata ttgttacacc tttgtgaatt
      241 ttaaccaaca ctcttataaa aattgttggt gtttttttag ttatcatgtc tctataataa
      301 ataaagtgaa ttttaaaagc aaataggagg aaatgtttca tgaaaaagat gatattaatt
      361 aatgtcatta cggttgttgt actgttagca attgggattg ctggctttta tttttggaat
      421 aaaacaacaa gctatgtaac aactgataat gcaaaagtaa atggcgatca aattaaaata
      481 gcaagtcctg catctggaca aattaaatca cttaatgtta aacaaggtga caaactcgat
      541 aaaggtgaca aagtagcaat tgttactgta caaggtcaag atggcgaaac gaaagatatg
      601 gatcttaaaa tgcctcaaaa aggtacaatt gccaaattag atggtatgga aggttcaatg
      661 gtgcaagctg gtaacccaat cgcttatgca tacaatttag atgatttata tgtaacagca
      721 aatattgacg aaaaagatat taaagatgtt gaagtaggta aagatgttga tgtgactatt
      781 gacggtcaaa aagcttctat taaaggtaaa gttgatagta ttggaaaagc aactgctgca
      841 agcttttcat taatgccatc atcaaatagt gatggtaact acactaaagt ttctcaagta
      901 atacctgtta aaatcactct agaatcagaa ccgtctaaac aagttgttcc tggaatgaac
      961 gctgaagtaa aaatccataa aaattaaggg ggtcattaaa tgactacgac cttcattatt
     1021 agctacatta ttttagcgct cattattgtt ggggttatca atttattttt aataagatca
     1081 agaaaaaaag gcaaacgcca acaaaaggaa caacaattta cgacacgtca atcaaatcaa
     1141 tctaaattta aagctagtga tttagacaaa acaactgatc aatcgacaca acgtatgacg
     1201 catgaagagt tgcgtgttga caatcaagat gatcatagcc aagttagtct aaatggttac
     1261 acaaaggggt ctgagaaaga tcaagaagca ttcactaata ataaggatga ggaagcagtt
     1321 gctgctaaaa atcctgaatc agaagaatat aaagtgaatg aaaaaataaa aaaagaacat
     1381 aaaaacttta tttttggtga aggtgtttca cgcggtaaaa tattagcggc attattattc
     1441 ggtatgttta ttgcgatttt aaaccaaaca ttattaaatg ttgctttacc aaaaattaat
     1501 acggaattta atatttctgc gtcaacaggg caatggttga tgacgggatt catgttagta
     1561 aacggtattt taataccaat tacggcgtat ctatttaata aatattcata tcgaaaatta
     1621 tttttagttg cgttagtact ctttacaatt ggttcattaa tctgtgcgat ttcaatgaat
     1681 ttcccaatca tgatggttgg acgtgtatta caagccattg gtgcaggtgt actaatgcca
     1741 ttaggttcaa ttgttattat tacaatttat ccacctgaaa aacgtggtgc tgccatgggt
     1801 acaatgggta tcgcaatgat tttagcacct gcaattggtc caacattatc aggttatatt
     1861 gtacaaaatt accattggaa tgtgatgttt tacggtatgt tcattatcgg gattatcgct
     1921 attttaattg gttttgtatg gtttaagtta tatcaatata caacaaaccc taaagctgat
     1981 atcccaggta tcatttttag tacgattggt tttggtgctt tgttatatgg tttctcagaa
     2041 gctggcaaca aaggttgggg ttcagtagag atagaaacaa tgtttgcgat tggtattatc
     2101 tttattattc tattcgttat tagagaatta agaatgaaat caccaatgtt gaatttagaa
     2161 gtattgaaat tcccaacatt tacattaaca acaattatta atatggttgt aatgttaagt
     2221 ttatatggtg gtatgatttt attaccgatt tatttacaaa atttacgcgg attctcagca
     2281 ttagattccg gattgttatt attacctggt tctctaatta tgggtctact aggaccattt
     2341 gcaggtaaat tgttagatac tattggttta aaaccattag ctatctttgg tattgctgta
     2401 atgacttatg caacatggga attaactaaa ttaaatatgg atacaccata tatgacaatc
     2461 atgggtatct atgtacttcg ttcatttggt atggcattta taatgatgcc aatggtaact
     2521 gcagctatta atgcgttacc gggacgactt gcctctcatg gtaatgcttt cttaaatacg
     2581 atgcgtcaat tagcaggctc tataggtaca gcaatcttag ttactgtaat gacaacacaa
     2641 actacacaac acttatcagc ttttggggaa gagttagata aaacgaatcc tgttgtacaa
     2701 gatcatatgc atgaattagc atcacaatat ggcggacaag aaggcgcaat gaaagtgtta
     2761 ctacaatttg taaataaact agcaacggtt gaaggtatta atgatgcatt tatagttgca
     2821 acgatattta gcatcatcgc cttaatttta tgtttatttt tacaaagtaa taaaaaagca
     2881 aaagctacag ctcaaaagtt agatgcagat aatagtatca atcatgaata aataaaataa
     2941 attaattgaa gtgtgactaa tcaaaaatta tgttgtgggg acatgatttt taaagtatcg
     3001 gtgccaaata tggttatcga tacttttttt atttgttgat ttatagaatg ttagaggaat
     3061 tatattaaaa tttggcattg acgtagtagg tcattaataa agaaaaagca ggaagtgggt
     3121 caacgaaatg aattttgtga aaataacatt tctgtcccaa tccctactat ataacattat
     3181 tttaaacgag gcatgcgatt acggaagaat aagcttataa caagtaaacc gatgctacag
     3241 ccaagtaaaa tgatgccgtt atgaatagcg tcagcttctg taatcacttg atctggtggt
     3301 acatttaaat aatatttttt gaaaacatct gcaatttctt gtgcgagtgc tttaataata
     3361 taactgaaat acaatgtaat gactaacata attgttaaca ctaaaatatt tactactttt
     3421 agaactgtgc gattaaatac aattgtcagt attgttatga ctaggctaac gatacataaa
     3481 ataaagaaac cgttaaagac taaaattaag cgatcgattc cttgttgtaa atcattgata
     3541 ttacccaaat taatatttaa ttgatgcatt tttccaattg tctcgttaaa atttttaatg
     3601 ccatcaaaat ctacagattc tttggtgaag acaatattaa aaatgggttg tttccacatg
     3661 ctgtatccag cgattgcggt taatataaca acaatgattt gaataatcat gcttaccaat
     3721 gaacgttttt tcttaccata tcgcttacca cgatgaatat tcggttgttg aaatgtctca
     3781 tcaaatggtt ctaatcgttg atttggattt gtcatgcaag gtacctccgt ataaagttct
     3841 tttctttact tatcaatatg tctagttgtc tgttataatg atgaaaaatc actgttcgat
     3901 tgtagtctta aatttgaaag tgaaaaagaa aatatcttct acattatata ctttttttgg
     3961 caaagaatgt aggggataga caatcattgg atacataaaa tatataaagg taggttaaga
     4021 cattgattag aaaccgtgtt atgaatagcg ttgtcaataa atatttgctt cacaatcgct
     4081 cgattatgtt taaaaatgat caagatgttg aaagattttt ttataaaaga gaaattgaaa
     4141 atagaaaaaa acataagcag ccttcaacat taaatgttaa agcaaattta gaaaaattgt
     4201 cattagatga tatgcaagtc tttcgcttta atttcagaca tcaaattgat aagaaaattt
     4261 tatatattca cggtggattc aatgcactac aaccatcacc gttccattgg agattgttgg
     4321 ataaaatcac tttaagtaca ttatatgagg ttgtactgcc tatctatcca aagacaccag
     4381 agtttcatat tgacgatact ttccaagcga tacaacgtgt ttatgatcaa ttagtatctg
     4441 aagtaggaca tcaaaatgtc gtagtcatgg gtgatggttc aggtggtgca ctggcattat
     4501 cctttgtaca atctctttta gataatcaac agccattacc gaataaattg tatttaatct
     4561 caccaatttt agatgcaaca ctatctaata aagatatttc ggacgcctta atcgaacaag
     4621 atgcggttct aagtcagttt ggtgtcaatg agattatgaa aaaatgggcg aatggcctac
     4681 cattaacaga taagcgcata tcgccaatta atggcacaat agaaggattg ccaccagttt
     4741 atatgtttgg tggtggacgt gaaatgacac atcctgatat gaagctattt gaacaaatga
     4801 tgttgcaaca tcatcaatac attgagtttt atgattatcc taagatggta catgattttc
     4861 caatttatcc aattagacaa tcacataaag ctattaaaca aattgccaaa tcgatagatg
     4921 aggatgtaac acaaaataac taataacata taaatgaccc agttacgatt tcatgcaaga
     4981 tgatatttga catgaaaacg taattgggtt ttgtttttga aaatatagca agggttatat
     5041 agactaattg ttaatgagct tctgagtgaa aatcaccaat aagtgtattg atatgtgtga
     5101 tgataaagta ttgcccttta atttgataaa attgatctaa aaaattatcg aattgacgtg
     5161 gaatatcact aagttctgca aagttagctg ttttatcata attgcgatga gcaacctcat
     5221 tttgaaattt atcataatgt tctaggaaat gttgatcttc aagctgttgt tccacaatag
     5281 tcataatatt atcgatggct tgcttttgtc cttgaacata agccatatgc acgccacgct
     5341 taagtagctg atttgctggt ccgttaatgt ataacccttt ttgtgcattt aattctgtta
     5401 attcaaactt aacatcatct aataaatcta gtaattcgat atgaatcttt tgttgtaatg
     5461 catctgtcat tttaatgctc ctttgtgtgg aattgctatt ttatgtccta attgttcagg
     5521 ccaagtgata gttgattgat ttttataata gtgtgctatt tgtgttgaaa atgattcagg
     5581 aaaagcatca gaacgacgtg tgtgctgatt aattcccatc atcattactt catttgttga
     5641 tgctagtgta ccatcctctt tagttaatgt taaaaataaa tgcaaccgct tataatcgta
     5701 atcataaata tataaagtaa cagtaaatac atcgccaaga gacaattctg agaggtatgt
     5761 cgtatgttct tctagtgtaa atagcgtata tgctaaattt tcgcgttctt ttaaagaaag
     5821 accgtggctg taattaaaac gattcacgac gtcactaaaa atgatattat aatttgcatc
     5881 atgcatatga ttgttatggt caatgaattc agatgttacg gtttgagtat gtgtaaaaag
     5941 ttgtttgata ataaccatct ccttagtgac tatcatacta aaataaatta agacatacac
     6001 gttatttaat tcatgataaa ataatagaga attcgttatt tagggggagt atcgatggct
     6061 ttaaagatac ggtatcaaac tacatatgaa ccgtttaaag tagttgatga tatcaaagag
     6121 ataccaaaag atgcgactat tgtttggtat gattttgatg aaccgaatga acaagaaaat
     6181 gaatggttta aagcacattt taattttaat gatttagaag tggatgatgc aattaacggt
     6241 atgccacgag ctaaatataa atcttataaa gattatcaat acttagtatt ccatagtatt
     6301 atggggtcaa actattcacc aatagcacta aatattttta tacaagacaa tgtattagta
     6361 acatatcatc atcaaacgtt ggaatcatta aataaagtag tctataagta tatgaataca
     6421 ttagatgcag aattagattg tgcagatgtc gtcatattaa ttttagatat gatggttgat
     6481 aaatatttca actttgtata tgcacttgaa gatagtgttt atcattttga agaccgccat
     6541 gtagatgatc gctttaataa aatggtcatg gatagtgtct ttaagttacg ttcggatttg
     6601 attaaagtaa aacgtgtact gtttcctatg caagagctaa ttgatacaat gaaacaaaat
     6661 ggtgatttga ttatagataa taaacattcg ctatacattc aacatatcga tgaccacttg
     6721 attaagcaac gtaatatcat tcgtactgct caagaaatga caaacgaaat acgagaaaat
     6781 tttgagtcat acacgtcatt tagaatgaat agtattatgc aagtactgac attagtatca
     6841 gttatatttt caccgttaac attcatagct gggatttatg gtatgaactt tgtaaatatg
     6901 ccggcattac atttacatta tggatattat atttgcttag ctgtaatgtt tgttatagct
     6961 gtcgtattaa tcatcttttt cagacgtaaa aaatggtttt aaattagaaa tgacgaaaag
     7021 gtaggtttaa tcatgagtga ttttcaaaga gaacagagaa aaaatgaaca tgttgaaata
     7081 gcaatggccc aatctgacgc aatgcattca gattttgata agatgcgttt tgtgcatcat
     7141 tctatcccat caattaatgt aaatgatatc gatttgacat cacagacgcc tgacttaacg
     7201 atggcatatc cggtttatat taatgcaatg acgggtggta gcgagtggac gaaaaacatc
     7261 aatgaaaagc tagctgtagt tgcaagagaa actggcttag cgatggcagt tggatcaaca
     7321 catgcggcat tgagaaatcc acgcatggct gagacgttta cgattgcgcg aaaaatgaat
     7381 cctgaaggca tgatttttag caatgttggt gcggacgtac cagtagaaaa ggctttggaa
     7441 gcagttgaat tacttgaggc acaagcgtta caaatccatg ttaattctcc tcaagaatta
     7501 gttatgcctg aagggaatcg tgaatttgtg acttggttag ataatatagc gtcgattgta
     7561 tcacgagtgt ctgttccagt cattataaaa gaagttggat ttggtatgag caaagaatta
     7621 atgcatgact tacaacaaat aggcgtcaag tatgtcgatg ttagtggcaa aggtggtact
     7681 aactttgtag atattgaaaa tgaacgtcgt gcaaataaag atatggatta cttatcatca
     7741 tggggacagt ctacagttga gtcattactt gaaacaacgg cttatcaaag cgaaatttca
     7801 gttttcgcga gtggtggttt acgtacacca ctcgatgcaa ttaaaagttt agcacttggt
     7861 gcaaaggcaa ctggtatgtc acgtccgttt ttaaatcaag ttgaaaataa tggcattgca
     7921 catacagtag cttatgtaga atcatttatt gaacacatga aatcaataat gacgatgtta
     7981 gatgctaaaa atattgacga cttaacacaa aaacaaatcg tatttagtcc ggaaatattg
     8041 tcatggatag aacaacgtaa cttgaatata catcgaggat aaataaaaga ggaggcgaag
     8101 gcctcctctt ttatttatga gtttggtgtt taactaaacc attgtatgaa tcccataatg
     8161 actataacac caattaaatc gattaagaat gcaccaacaa taggtacaac taaatatgct
     8221 ttaggtgagt ttccatattt tttagtaata acatctaaat ttgccatggc atttggcgtt
     8281 gcaccaagcc catgaccgat aaaaccacct accattactg cagcatcata atcttttcct
     8341 aaacctctaa ataaaattaa aacagcaaat aaaatcataa cgacaacttg aactaaaacg
     8401 ataataataa gaggtatagc aagtttataa atttcgatta attgaatgct cattagcgca
     8461 agagataaga aaatacctaa tgcgacatcg ccaatttgat tagtaatttt taaatctaca
     8521 atattaaaat taaaactttc agagatattt cggacaataa cagctacaaa taatgagcca
     8581 acgtaaattg gaacattaat ccctgtttga gctgtaaaca aatgactgaa ataacttcca
     8641 actgccatac agaatacaac gattgtaaat tgaatgaaga atacttcagt ggcattaaat
     8701 ttactatgta aatgttcgtt atatgctact tggctataat ctttaaatgt atcgtcacta
     8761 tgttgaggct ttaagttata acgtttgatt aaaaatttta caactggacc accgataagc
     8821 cctccaaata caagacctaa agttgcagct gcaagagccg ctgtcagtgc cgaatcaata
     8881 cctaaatctt gaattgtctt accataagca gcagcattac catgaccgcc ttccatagac
     8941 atggaacctg ctgttaatcc taacaaaggt ttaatattta atacttttgc tagtgataca
     9001 ccaactatgt tttgaatgac tgaaatgata gcacaaaaca taaagtataa tagcaagact
     9061 ttgccaccta atttaaataa tttcaatgat gcaccaagac cgattgtcgt aaagaatgct
     9121 aacatgaaga aatcttgaat gaatgaagca tctaatttaa tcttaaccat gccaaatgaa
     9181 tccaaagccg caactaaaat agcaaagatt aagccgccaa tcactggtgc tggtatacaa
     9241 atacgtttta aaaaattaac gtgattaacg atagccttac caagtaaata aaggatacaa
     9301 gctaaacata atgttgtaat tgcattaagt tctatcattt tcaaatcccc cttgttgaat
     9361 atccctctcc ctaaataagc ttttatatgt aagcgttata aaagggcgct tttaacatta
     9421 tacatgcaac aaatcaatat ttctagtttt tgaaaataat atcaataata ttaatgcatc
     9481 taaatttaat aattataaat aattctattg ttatatttta taattaagaa caagttcaat
     9541 aagaggagat gaatcaaaaa atatgatgga tacaaagaaa aacaatcaac ccatccgcat
     9601 gttgacggca cgattgattg acttataagg agatagattt cattttcatg atagtaatca
     9661 atgaaagatg gcatttattt ccaagtatct tcgggaaaca tacaatctga tttacgcatg
     9721 cgagacacaa atggattacc tttcactgtg taacgtaaag atttatgtgt ccaatcacct
     9781 ttatttggaa taccgattcg tggactagca ataatatctt taggatattt acgattctta
     9841 gtatcaatag acaatctaca gtcatttaac gtagcgccat cgatagcccg tggaatgtta
     9901 aatgccttag tccattttcc tgggccattc gttacctcgt agcctttctt acctctgtta
     9961 cggaacatag cggataaacc ttcttctggt tcaattgcgc ggataagtac gccttcaggt
    10021 ataccttcag attttgttac aaaattaatg agtaaatgcg tatgcatgac atgtgcataa
    10081 attgtaccac cacgtttata taatgacgtg actttaggtg ttattttacc gccatagcca
    10141 tgagccgcac gatcattcaa acctaagtaa gcttccgttt ccacgatgta gcctgtatac
    10201 gtttgagtgg tatcctgata aatcactttg acacctaata agtttttagc aatttgtctt
    10261 gtatcattat taacgaaatc cataatattc tcctcgcaat tatatgatta atttaaataa
    10321 aaatgaaaaa gatattttta ttgtggtaaa tgtttgataa aatagtattg ttaagaatta
    10381 taaaaatgta aagtatatac tttgttatgg aatattttat ttgcatagat tgagactctt
    10441 ttaaacgtga acattgcagt atgacatatt tttagtatag ttaacgaagc gttgactttc
    10501 aaatgagtct ccatatagat gtgtacttgt agcgggtttt cattttacta gtgatgaaat
    10561 ataaagttca ttttccaaag tgattgacga ttgaaatgaa gtggcttgat aatgaatctt
    10621 gtaaattata acggtaacaa taattaaaaa tataaatatg attaacgaaa gggagattac
    10681 aatggaacaa ttacctcaag taggttttat gaaatcattc gttttatttt ggaaaaatta
    10741 tgtaaatttt aaggggagat caagacgtag tgaatattgg tatatggcac tatggcattt
    10801 gatacttatg gttccagctg tgtgtgtgct aataataaac ctatttctat tctttatttc
    10861 tatagcggcg gataacgaaa cattaatagt tatgagtata ttgatgatta tccttacatc
    10921 tgtgtatact tttgtctata gtttggcaac gtttattcca aatttagcgt tgtgcgttcg
    10981 aagatttcac gatatatcaa gaacaatgtt atttccaatg attatgacag tattcagtat
    11041 tatattttat atagtattac agattatcga tagttattat gacagtgatt ttacattaat
    11101 gccgatgggt cttaatattt tgttagtact tctttatttt gtttatttag gcctaacaat
    11161 tgttatgata gtattcctat gctttgacag taagccggca aataaatatg gcgaaagccc
    11221 aaaatacccg tctacaatta aaaatcaacc agtgacatct gaaacaccgg aaaaagcgtg
    11281 tacatctgaa acaccgaaaa caccggaaga aacagataat caattgtaat gatataaata
    11341 tctcggcaca atgatgctgg gatatttttt tacaaataag gcaataaagg catggaaaaa
    11401 acatatctag tgctaatgat acttaacatg caaaaatggc actatttaaa tttttcaatt
    11461 tcagaaattt taaaaataaa ttcattttaa aaatattgca tttatagaga atggaaatgt
    11521 tatattaaga taaatacata tttttttagg aggcaaagta tgggacttgt aattgaacat
    11581 gttacgaaac gttttggcaa gatgacggca gtaaatgata tttcattaaa gttagaatca
    11641 ggtaaaatgt taggttttct aggaagaaat ggtgcaggta aaacaacgac atttcgtatg
    11701 attttaggct taagtgaacc aactgaagga cacattacgt ataacggtaa aaaactagat
    11761 aaaacaatgt acaatcgtat tggttattta ccggaagaac gcggtttaca tgggaaattg
    11821 acagttgaag aagaattgaa atatttggca actttaaagg gaatgtcaaa aacagaaata
    11881 caacagcaaa tatcgtattg gcttgagcgt tttgatatta ctgaaaaccg caaaaaacga
    11941 attgatagtc tatcaaaagg gaatcaacaa aaaattcagc tgttagcaag tatgttacat
    12001 aaaccagaat tactaatatt agatgagcct ttcagtggtc tagacccagt taatgttgag
    12061 ttattaaaag aagcagttaa agacttaaat gattggagca gtacaatcgt ttatagttct
    12121 caccgtatgg agcatgttga agaattatgt gatgatgttt gtattttaga taaaggtcaa
    12181 cttgttgttt ctggtgatat caatcatgta cgtgcatcta atggtaacaa gaaagttgtc
    12241 attgaatcag aaacaacact accagattta acaaacatta gaggcatcat tcatagtgaa
    12301 aacatgaagc aaggtcttca gttgacgatt gagaatgaag acgtagccaa agatatttat
    12361 caagttgtgg cacatcaagg ttatgttaaa cgattccaag ttgtagaacc atcattacaa
    12421 gatatattca ttgagaaggt tggtggcaag gatgcataag ttttgggcga catttacttt
    12481 gacatatatc aaaaaaatta aagctaaatc atttgtaatt tttatgatta ttattgctgc
    12541 cttaatgatt ggcctatcta acattgataa gattattaac atgtttgatg atggtccaga
    12601 taaaattggt gtagcagcac cgaatgaaca aatctataag gttttcaagc aacaggcgaa
    12661 tacatttcat agtgatgcta agtttactaa agtttctata gaagatgctg aaaaagaagt
    12721 aaaaaaacat aaacttgata aagcttatat tatcaaggtg aatcaaaatc gcacgttaca
    12781 agggacaatc attagtgaga agcgagtaag tcatgaagat tcacaaaaag tacaagcact
    12841 gcttacagct attcaaacaa atatggtagc cggggaatta aatatcaaca aagaagactt
    12901 acaaaaatta caagcacaaa gtaaagttga taataaggtg atttctaatg atgaagtcga
    12961 taaagtatca gaaggacaaa agattttcaa ctatgcttta gcttatggca ttatctttct
    13021 aatgttcttc attgttttaa attatgcatc tcaaatagct atggaaattg ctagtgagaa
    13081 aacatcgcgt gtcattgaaa tgattattac aagtatttca ccaatccaac atatttttgc
    13141 taaaatttta ggcgttatag cagttgccgt gactcagatt attttaattg tattgatggc
    13201 agttatatgc atttttgcct ttgacttaaa agacttgcta caaggtttta acgtggaaat
    13261 gaatcaattg tcttggcaaa ttattattgt cggaattata agtagtattg ttggaattac
    13321 ggcgtatgta ttacttgcag ccatcctcgg atcattaaca tctagaatag aagatttaaa
    13381 tcaatctttg atgccactga cattacttgg tatgattgca ttttatatcg cgatattcac
    13441 gataaataat cctgatggac aacttgcttt aatcacaagt tatattccgt ttttagcgcc
    13501 atttcaatta gttgtacgcg ctcaaacttc agggcttcaa atatatgagg tcatcttaag
    13561 ttctttaatc tcaattgtaa tggtagctgt cttgatatgg attgcaatta aaacatataa
    13621 agattcagta cttacatttg aacgtggctt atttaattca atgaaacgtg tatttaaaaa
    13681 ataaaattga tgagagtgac acacatttat catataaaat gatgatgaaa gcgtgttact
    13741 cttatttttt tatatttagc tatagaataa tgtctaaaaa agtgtacaat atgaatgtga
    13801 aaacgaactt tttaaaatgg gaatgtgaag taagaagaaa tgtaaacaaa tgatatgtag
    13861 ccgatagatt attgatagca aatgtgtgat ggagtgattt catgttatat ccgattttta
    13921 tatttatatt agctggctta tgtgaaatag gtgggggata cctgatttgg ctgtggctta
    13981 gggaaggaca gtcttcactt gttgggctaa taggcggtgc gatactcatg ttatatggtg
    14041 tcattgcgac atttcaatca tttccatcat tcggaagagt atatgcagca tatggtggcg
    14101 tatttatcat catgagtttg atttttgcga tggtcgtaga taaacaaatg ccagataaat
    14161 atgatgttat cggtgcaatc atttgtattg taggtgtttt agtaatgttg ctacccagca
    14221 gagcttgaac acttgtggtt agaagggaga agtaaaaatg atagttgaag ttgagcacca
    14281 aagacaaggt gtagatttaa tcaaaattga taatgatgaa actaaaatta tttttacgaa
    14341 ttatggagca agagtagtgt cttggaaata tcatgataat aatattgttc ttggaaatgt
    14401 tgttgaagcg gatgaatttt atcatagtaa tccttttaaa tttggtgctt ctataggaag
    14461 atatagcggt agaattgata atgctaaatt taagttgaaa ggtaaagaat atcagttaga
    14521 aaaaaacaac ggtgaacatc atttacatgg tggatgtcat ggtttggaca ataagttatt
    14581 tgattatgaa attagaaatg aaattgctca aattaaagtt atatttaaga cggtgttgaa
    14641 atcagctgat gatcatttcc cgggtgatat cgatgtgacg attacacata tttatgatgc
    14701 ggatcaccaa tggtcgattg aatacgaagc agttgcatca gaagacacat tgtttagtcc
    14761 tacgaatcat gtgtacttca atttgaatcg agataataat gtcgttgata atcatcgcat
    14821 ttcaagtaat caattagata tgtatgtatt agatgagcga aatattgtta caggtgatat
    14881 tctagattta catgaagtgt ttgaagataa caagataaaa ttgagtgata tttttacatc
    14941 acaacatgcg caattaagcc aacaaatgac acgttttggt ggtctcgatc acccatttac
    15001 agttggtgaa cataaaatgt atgttgaaaa tcatgaattt atgttagagg tcgatacaga
    15061 tatgcctcat gttgtgttct ttacttttaa ccagcctgat gagtgggata gtccatttaa
    15121 tatttataaa ccacattctg gctttacatt agaaacacaa tttttaccaa atgatattaa
    15181 tttatatggt gaaaaagcac cgtcaatttt aaaagctggt gaagtgttca attcgaaaac
    15241 aagtttccgt atcgaagaaa aagtagaaaa tgattaaaag tatcggcatt tatataatat
    15301 ttatgttatt tggcggaatt ttgaataggt tatcaagaat cgtatcaaat gaagtgacat
    15361 cattaatata cagtttgaaa taaagaaaat atataacata aaaaaggaat caatagcatc
    15421 agtggttata gataagacta ttatacaaac gatgtgtaag ttgattcctt tttgttttga
    15481 aaagttcatt taacacttat aatgaaaggt aattatttca atgttctgtt atacattttt
    15541 tgaagtttat cgcgtcttgt tggtgacaga aatggattgt ttagtgcata tcgtaacata
    15601 tcttgatttt gtctatcatt ataataaagg tcattaagct gttgtacaga taaacgatgt
    15661 tcgattggtg cttcttgtat taagtaaagc tgatcatttg gagatacata gccttgtttt
    15721 aatacccgga aatagaaacc tgttttacca gatgtagaca tgcgcttcac taaatcagga
    15781 atattatatt ttgcttgaat tttccagcat ggttctctaa tttcagaaac ctcaattaac
    15841 gcttcaccta aacgatattg attaccaaag taaacatcag attcgtctaa ataatcaaat
    15901 gttaaattct ctccaaacat cgcatgagta ggtagtgtag gtaaatcatc ttgatacagt
    15961 gcataattat gcttactaaa cccacaaact gctttatgtg gtccaccatg atctttataa
    16021 gcctgttcat caccagtgaa accagttgaa gataaccatg ttgtttgtga aatcttagtt
    16081 ttatctaaag cagagcgcat cggtcttttc gagctataag gcaaatcttg gatttttcct
    16141 gtcgaaattg cgtgaattga tatcatatca ttctccttaa cgagtattat ttaaaatata
    16201 ttgtactata aaacattttt agaattgtaa attctgattg atatgtttaa aaaaatgtta
    16261 gaaataaggg tatgttaatt tatacgagtc atgtaaaata tgaattgaat agattacata
    16321 aatatgggag gctaccttca tgaaagatgt caaagcactc aagttaatga cattaaatga
    16381 tgttttaagt caaatcaatg gtgacatgac gctgggaatt ggtacaggaa gtacaatgga
    16441 attactctta cctcaaatgg cgcaactaat taaagaacgt ggttacaata ttacaggtgt
    16501 atgcacttct aataaaattg catttttagc aaaagaatta ggtattaaaa tatgtgaaat
    16561 caatgatgtt gatcatatcg acttagcaat tgatggtgct gatgaagtag atccatcttt
    16621 aaatataatt aaaggtggcg gtggtgcgct gttcagagag aaagttatag atgaaatggc
    16681 atcgcgtttt gttgtggttg tcgatgaaac gaaaatagtt caatacttag gtgagacgtt
    16741 taagttacca gtagaggtgg ataaatttaa ctggtatcac atattacgta aaattgaatc
    16801 atacgctgat ataaaagtag aacgtcgtgt aaacgaagat gtcgcattta taactgataa
    16861 tggcaattat attttagatt gtaagttacc aaaaggaatt gacccatata agttccatga
    16921 gtatttaatt catctgacag gtgtgtttga gacaggatac ttcttagata tggcagatca
    16981 agttattgta ggcactcaag agggtgttaa aattttagaa aaataatata tagtatagaa
    17041 aagcgcttgc tagcatcatc tttttaagag gtactagcaa gcgctttgtt attatttgtt
    17101 tttatctgaa gattgttctt cagttttttc gatatgtttt tctgcatcaa ttacaactga
    17161 tgaacgtctg tcttctttat aagtcgcagg ttcttttaat gattgctcta tttcatcttc
    17221 atttgaatcg tttgttaaat cgtcactttg tggtgcttgt ttaactgatt ctgctgattc
    17281 tactgactcc gaagtgatgt catgatcttc atcatgatta gattcagttt gattacctat
    17341 gtgatgttga ggttctgttt tttcagtacc ttcatgaatg cttggttcgt cagccactgt
    17401 attttcagta tcatttttag caacaccaac cgttgatgca gtagctacac ctgttttttc
    17461 agcatcttta atatttgaag aagatttttc agcctcagta tgattagttt cttcatcgtc
    17521 attgacatgg tctaaataat tattaggctc aagttcttca aggtttcgtc ttgttgttaa
    17581 atatgcaata cctcggataa ttaagcttaa accaatgtat cctactgcaa cgattgctgt
    17641 tgaaatggcg atgactttga ttgaaaatag atcgccgatt tcttcgctaa acaagaaaat
    17701 aagtccgaat gtcattgaag catggaatgt cgttgcaata taaattgtac gtcctttagt
    17761 cgctctaatt aattcaccaa gaatcataga gaatgagaat gtataaagga agttataagc
    17821 agcaaattct gtaccataag ttgtatttgc tgagaaaact gaatacatca aaccaacaac
    17881 aatacttgca aagaacgtgt tcattttcgt ttcaacaata ttttgtaagt atgaacggaa
    17941 tccgaattct actacgaacg ccatcagaat atgtccaatc agaatgtgtg taataggtac
    18001 tgataagcct gttgattgta ataaaataaa gctatctgca aatgtattaa agctgtacat
    18061 accaataatt agaataatta aaggtaaaat taaagctagc aatagacgtt caattacttt
    18121 caggctgatt gagaatttta aacctgcaag ttggaccttt ttatatttga aaacgagtat
    18181 acaaatgatt gcagcaatta atggtgctag atctgtaact tcaaatataa agtgtttgac
    18241 accaattata gactggaaat ctctgagcat aatggataac gccattgtaa tgacaaagaa
    18301 gacgaaaatc gtcattgccc attgaaaacc agaaatttta ttgttcttca ttatatgtaa
    18361 cctccattag gtaacaagca aaatatcttt tagtaacaat tatacatatt acaagccgag
    18421 agataaattg tctatgtcaa tattaatgaa aatgtaatag tcttgacgta ttttattaac
    18481 ataaatcagc taagtattcg ttattacata gaaaataggg gtaaaagtaa tgtagataag
    18541 tatttttaag atacggattt aactatgaat tgaattcaaa atttcctata cgttatactt
    18601 taattgttaa taagccacat aaataagagg gggaaatgct gtgtacaagc aaggtgaacc
    18661 aaatttatgg actggaaggt tagatagtga aacagacccg aaaaaattta gacattttca
    18721 aacagtaaca tttgaagatt tgtctaagct ggaaaagagt agtatgccat caggggtcgg
    18781 tatattaggc tatgctgttg acaaaggtgt tgctttaaac aaggggcgca ttggtgcaaa
    18841 agaaggacca gatgcgatta aacaagcatt tgcaggtttg ccggatttga atcaatgtga
    18901 aactttagtc gattacggaa atgtttatca tgatcatgag gaattaattg atactcaaaa
    18961 agaatttgct atgcttgcag cgaagtcaat tgctaatcat agacaaacat ttttattagg
    19021 tggtggacat gatattgcgt atgctcaata tttagcaaca cgtaaagtct atccaacaca
    19081 atctattggt gtgataaata ttgatgcgca ttttgataca cgtgctgaac aacaatctac
    19141 atctggaacg agctttagac aaattttaga agaagatgaa aacacagatt atttagtgct
    19201 tggtattgct caaggtggta atacgcaaag tttatttgat tatgctaaag agaaaaagat
    19261 tgattatgtc tttgcagatg aattattgag tcacgtatca ccaacaatta aagatatgat
    19321 tgaacgtttt gtacatgaac atgatgtcat tatgtttacg atttgtatgg atgtcattga
    19381 tagtgcgttt gcgcctgggg taagtgcgcc ggcagtgtta ggtttgtatc cacatactgt
    19441 tcttgaatta gcaaaacgta ttattccaag tgataaggtg tcttcagtta gtattgctga
    19501 gatgaatcca acatatgacg ctgacaatag aactgctaag ctcgttgcta atttagtgca
    19561 tcatttttta aaataattaa aaatatttat atgtgacgag atttgcattg cagggcagaa
    19621 tgtgagtatt tcttaataga agttatatta acgttgatat atttgaa
//
LOCUS       DAKTIO010000033        19482 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.033, whole genome shotgun sequence.
ACCESSION   DAKTIO010000033 DAKTIO010000000
VERSION     DAKTIO010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19482
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.033"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            222..1094
                     /locus_tag="O0293_000891"
     CDS             222..1094
                     /locus_tag="O0293_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCY0279785.1"
                     /translation="MSNVLTEIDSQYPYMTKNEKKIAKFILNSPQKVIKMRSQDLASL
                     LDISTSSVIRFSKKITDGGFHDLKINISKYVPKASSIYNVELMNNESTESLRTKLHTR
                     TTRALNHANNELNDKTIDQICHCLKRSETIFIYGFGASFVVATDLYQKLSRIGLNIQL
                     VQETHIFATLLATHNSNDSVILITNNGTQSEMQSMVKVIDDYHIPIITITSTRDNPVA
                     QASNIVLTYGKTDENEMHMGATTSLFAQMFTIDILYYRYVALNYHASLDFITQSKMAL
                     DNYRKHLSNINFKH"
     gene            complement(1481..2860)
                     /locus_tag="O0293_000892"
     CDS             complement(1481..2860)
                     /locus_tag="O0293_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0279786.1"
                     /translation="MENNELQRGLSARQIQMIALGGTIGVGLFMGATSTIKWTGPSVI
                     LAYLIAGIFLFLIMRAMGEMIYLNPTTGSFATFASDYIHPAAGYMTAWSNIFQWIVVG
                     MSEVIAVGEYMKFWFPELPTWIPGVIAILLLMAANLFSVKAFGEFEFWFALIKVVTII
                     LMIIAGFGLIFFGFGNGGHAVGISNLWTNGGFMPNGIVGFFFALSIVIGSYQGVELIG
                     ITAGETKDPQKNIVKAVNGVIWRILIFYLGAIFVIVSVYPWNQLGDIGSPFVATFAKI
                     GITFAAGLINFVVLTAAMSGCNSGIFSASRMIYTLAHKGEMPKIFTKIMRNGVPLYTV
                     VAVSLGILIGALLNVILPLYIDGAKSIFVYVYSASILPGMIPWFMILFSHLRFRKLHP
                     EELEGHPFKMPGGAVTNYLTILFLILVLVGMVFNVETRISVLIGVIFLTIVTIYYFIR
                     YNKNNVKAK"
     gene            3140..3496
                     /locus_tag="O0293_000893"
     CDS             3140..3496
                     /locus_tag="O0293_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279787.1"
                     /translation="MLKQILLRATKISVLFAVAFFIINYIGMEKPDILYLVGRTIIAT
                     LAFILICLTVFTIINSPERKIKLGTTLPIALIIGIIFGAIFLTVQIGVITGLIIGVIA
                     TFIWELIEKNKGGRSS"
     gene            3493..3678
                     /locus_tag="O0293_000894"
     CDS             3493..3678
                     /locus_tag="O0293_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279788.1"
                     /translation="MTTALIGVIVLILLIISFVPNYRAMKSAKEQGSNATRATIMVGI
                     DIILIVLIIVTLLLKYM"
     gene            complement(3774..3965)
                     /locus_tag="O0293_000895"
     CDS             complement(3774..3965)
                     /locus_tag="O0293_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279789.1"
                     /translation="MMGYIILFFLAGPVILGVGNLVIGPIFNKQTPFRVQVRSFVVGS
                     MIYLILATIGYFLLLQGKL"
     gene            complement(3976..4611)
                     /locus_tag="O0293_000896"
     CDS             complement(3976..4611)
                     /locus_tag="O0293_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCY0279790.1"
                     /translation="MYRAVIFDFDGTIIDTEQHLFNVINKHLEMHNADPISIDFYRSS
                     IGGAATDLHDHLIKAIGSENKDKLYEEHHLTSTTLPMIDTIKSLMAFLKQRHIPMAIA
                     TSSVKAEIMPTFKALGLDDYIEVVVGREDVEQVKPDPELYLSAVQQLNYMPTQCLAIE
                     DSVNGATAAIAAGLDVIVNTNKMTSAQDFSNVDYVAKDIDYDQIVARFFTK"
     gene            complement(4686..5603)
                     /locus_tag="O0293_000897"
     CDS             complement(4686..5603)
                     /locus_tag="O0293_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HCY0279791.1"
                     /translation="MFQKLSIFATKSFLVWMLVAAVIGFIFPQHVATLGKWVPYLLGI
                     VMLGMGLTITPNDFKMVFKAPRAVIIGVCLQFSIMPTLAFIIAKSFHLPPDIAVGVIL
                     VGCCPGGTSSNVMSYLAKANVALSVSITTVSTLLAPFVTPALIYLFANEWLEVSFVSM
                     LWSVVQVVLIPIALGIVLQIINRKIAEKASTALPIISVVAISLILAIVVGGSKHQILT
                     TGLLIFLVVILHNVLGYTIGYWLARLLKLDRQDQKAVSIEVGMQNSGLAVSLAALHFN
                     PIAAVPGAVFSFIHNITGPILAKYWSKKL"
     gene            5871..6401
                     /locus_tag="O0293_000898"
     CDS             5871..6401
                     /locus_tag="O0293_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279792.1"
                     /translation="MLIDKFETYIINIAGLNDRTTRKKLSKLCKSVQFCDALQFSINK
                     QFNQYVLEISLPKQQLPYFISFLSFHQYSIFQVLSPKKINELLDSDNLYQSAKRFDIN
                     IDGLQDAFIKDKVIDIMNMFQNHTDITYTLNKSHAHIICTPEIFAKLLHTIATRNIDI
                     LSANYRSSSMSKARIS"
     gene            complement(6467..8071)
                     /locus_tag="O0293_000899"
     CDS             complement(6467..8071)
                     /locus_tag="O0293_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCY0279793.1"
                     /translation="MNAIKRFGSAMIVPVLMFAFFGIVLGFATLFKNPTIMGSLADQH
                     TFWFKFWSVIESGGWVIFTHMEVVFVVGLPLSLAKKAPGHAALAALMGYLMFNTFINA
                     ILTQWPHTFGANLEKGVENVPGLKSIAGIATLDTNILGGIIISAIITWIHNRYYSKRL
                     PEMVGVFQGLTFVVTISFFVMLPLAAITCVIWPTVQHGIGSMQHFIIASGYIGVWLYH
                     FLERVLIPTGLHHFIYAPIEVGPVVVNHGLKAEWLQHLNEFAKSTKPLKEQFPYGFML
                     QGNGKVFGCLGIALAMYATTPKENRKKVAALLIPATLTAVVVGITEPLEFTFLFIAPY
                     LFVLHAVLAASMDTLMYAFGVVGNMGGGLLDFISTNWLPLGKEHWGTYVAQVIIGLIF
                     VAIYFFLFRFLILKFDIPLPGRKKTEEEVKLFSKQGYKNKKGDSVDSKRASSGNEYED
                     KAAYYLDGLGGKENIKDVTNCTTRLRLTVYDESKVADTEYFTHQQMAHGLVKSGKSIQ
                     VVVGMTVPQVREAFEQMVEDQSSEDK"
     gene            8401..9165
                     /locus_tag="O0293_000900"
     CDS             8401..9165
                     /locus_tag="O0293_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCY0279794.1"
                     /translation="MFLDEHINRNFDKLNDNDLHIAHFINTHIDECKNMKIQDLAQFT
                     HASNATIHRFTRKLGFDGYSDFKSYLKFESNKTHQLPSNSIDSFKQEIDSTFSYLERV
                     DYQLLTKKMHTASTIYLYGTGRAQLNVASEAQRILLTLHNHVIVLHDEHELKMILNKS
                     EPNDLFFIISLSGETHELLEVTNLLQLRQKYFISVTTMKDNTLAQKANYNVYVSSNTF
                     YLTDGTDYSSFISYHIFFETLVRKYNEYLLHNDISN"
     gene            complement(9223..9729)
                     /locus_tag="O0293_000901"
     CDS             complement(9223..9729)
                     /locus_tag="O0293_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HCY0279795.1"
                     /translation="MAKFNVENEHVEVEIEKLYKFSPELVYEAWTKKDLLKQWFMTSA
                     RTNKEIEADVKEGGKYRIVDQQRNGKVNVIEGIYESLVMDEYVKMTIGMPGLSETQDV
                     IEVEFFERETGGTQMLFYYRSLVEKERRFTNLEYKQKKKEYHDAMVHGFELMFDKMYH
                     VIETSTQQ"
     gene            complement(9896..11209)
                     /locus_tag="O0293_000902"
     CDS             complement(9896..11209)
                     /locus_tag="O0293_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HCY0279796.1"
                     /translation="MEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITIT
                     VIVALLMNRKESFAKKVEVFTKGAGHSNIVLMMLIFILAGAFSNTAEKMGGVKSTVNL
                     GLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVV
                     GGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISL
                     NHAKNYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDA
                     VSKGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSV
                     ADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAA
                     GVAGISPLMLFPYSIYPILLGVCGLIAILFNFPKLKKNTSQEMKH"
     regulatory      complement(11286..11381)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(11434..12003)
                     /locus_tag="O0293_000903"
     CDS             complement(11434..12003)
                     /locus_tag="O0293_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279797.1"
                     /translation="MALIVSRAILFLILALAFGIATVFPEQKLPLYIVFTLFVAVAIV
                     SNVWFTHQNVKKHIIKFNKYVAYFLSFVSGLLYIGFVIYTLTIGSYDVLPIIIFAISI
                     VLVQILENILGISIKNTRLSNKQLKVISKQRVQQFKVISLILVSVSIVFIFIALMLKW
                     WLLLVISIIVLIIITVAMIVYEKYYTTPD"
     gene            complement(12490..13371)
                     /locus_tag="O0293_000904"
     CDS             complement(12490..13371)
                     /locus_tag="O0293_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCY0279798.1"
                     /translation="MAAQDPRTKFKTTDYEKQEQEVPGLQSEMTPAPDCGETSYQGHQ
                     RLQGYKILVTGGDSAIGRAAAIAYAKEGADVAINYLPSEEQDAQEVRQVIEESGQKAV
                     LIPGDIRDEQFNYDLVEQAYQQLGGLDNVTLVAGHQQYHDDIHGFTTEAFTETFETNV
                     YPLFWTVQKALEYLKPGASITTTSSVQGYNPSPILHDYAASKAAIISLTKSFSEELGP
                     KGIRVNCVAPGPFWSPLQISGGQPQSKIPTFGQKTPLGRAGQPVELCGTYVLLASEES
                     SYTTGQVFGVSGGVQID"
     gene            complement(13482..14603)
                     /locus_tag="O0293_000905"
     CDS             complement(13482..14603)
                     /locus_tag="O0293_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HCY0279799.1"
                     /translation="MGLKDQAVTWRRYFHQFPELSDKEFKTTQKIKDILTEHHIRILD
                     LPLATGLVAEVGQGLSCIAVRADIDALPIQELVEQDFKSENEGVMHACGHDIHMASIL
                     ATAVKLKEIEGTLTGRVKFIFQSAEELGHGAFKIIETHALKDVQAVLGFHNDPSRSVG
                     TFAIKTGAITSAVDRFEFHIKGVGGHAAKPEQCNDPVIVLAQLINSIQSIVSRNLSAF
                     DEAVVTIGQISCGNTWNVIADHAYVQGTVRSFDPVVRKLVETRLQDIADGLAQAYNMK
                     INLNYTHLPGAVMNDEALTHKAIAVAQHVGYKVEMMEQPLTIGEDFSGYSQHFPSVFA
                     LIGSHSEYDLHHPQYKPDERILEKVPEYFVEFVKRLLHE"
     gene            complement(14880..16118)
                     /locus_tag="O0293_000906"
     CDS             complement(14880..16118)
                     /locus_tag="O0293_000906"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HCY0279800.1"
                     /translation="MNDLIINHIAELILPRSTDKPLKGKELDELNVVKNGTVVIKDGK
                     IVYAGTHTDDYDATETIDASGKVVSPALVDAHTHLTFGGSREHEMSLKRQGKSYLEIL
                     EMGGGILSTVNATRETSEDDLFKKAEHDLLTMIKHGVLAVESKSGYGLDRENELKQLK
                     VSNRLAEKYDLDMKHTFLGPHAVPKEASSNEAFLEEMIALLPEVKQYADFADIFCETG
                     VFTIEQSQHYMQKAKEAGFKVKIHADEIDPLGGLELAIDEQAISADHLVASSDKGKEK
                     LRNSDTVAVLLPATTFYLGKEDYADARGMLDNNGAIALATDYNPGSSVTNNLQLVMAI
                     AALKLKLSPNEVWNAVTVNAAKAIDINAGTINTGDKANLVIWDAPNHEYIPYHFGINH
                     AEKVIKDGKVIVDNTVSFKA"
     gene            complement(16118..17779)
                     /gene="hutU"
                     /locus_tag="O0293_000907"
     CDS             complement(16118..17779)
                     /gene="hutU"
                     /locus_tag="O0293_000907"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HCY0279801.1"
                     /translation="MRKIQAKKGLSIECKGWEQEAVLRMLYNNLDPEVAERPEDLVVY
                     GGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEAPRVLISNSVLVP
                     EWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAELGNQHFNGDLAGT
                     VTLTAGLGGMGGAQPLAITMNHGVAICVDVDETRVDKRIDTKYCDVKTADLDEALKLA
                     EEAKERGEGLSIGLVGNAVDIHQAILEKGFKIDIITDQTSAHDPLNGYVPQGYSVEEA
                     KVLREKDPKKYVELSQASMAKHVELMLEFQKRGAVAFDYGNNIRQVAFNNGVKNAFDF
                     PGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKLFPENEKLLRWLDLAEEK
                     ISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLDAGSVASPNRETE
                     SMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGMVVVADGSERAER
                     RLERVLTTDPGMGVARHVDAGYDIAIQTAKEKGIHIPMIDKAGDK"
     gene            17939..18823
                     /locus_tag="O0293_000908"
     CDS             17939..18823
                     /locus_tag="O0293_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0279802.1"
                     /translation="MKIIQLEYFLAIVKYNSFTKAAQFLHISQPSLTATIKKMEADLG
                     YDLFTRSTKDIKITEKGIQFYRYASELVQQYRSTMEKMYDLSVTSEPRIKIGTLESTN
                     QWIANLIRKHHSDYPEQQYRLYEIHDKHQSIEQLLNFNIHLAITNEKITHEDIRSIPL
                     YEESYILLAPKETFKNQNWVDVENLPLILPNKNSQVRKHLDDYFNRRNIRPNVVVETD
                     RFESAVGFVHLGLGYAIIPRFYYQSFHTSNLEYKKIRPNLGRKIYINYHKKRKHSEQV
                     HTFVQQCQDYLYGLLEAL"
     gene            18945..19364
                     /locus_tag="O0293_000909"
     CDS             18945..19364
                     /locus_tag="O0293_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB1/FosB3 family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HCY0279803.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
BASE COUNT         6535 a         3802 c         2723 g         6422 t
ORIGIN      
        1 taatatatca atatattgat atattatttg cacatgggag tatgctattt tttatctata
       61 catcttaaaa aaatttacag ttttacaatt caatatcatt aaacaaacaa catattcagc
      121 ttatttttca ttctcttacg ttacaagttg taaataccat cgcgttaaac aacaatagcc
      181 aacgctaata aaatatattg cgttaaagtg gagtgattaa tatgtcaaac gtactaacag
      241 aaatagatag tcaatatcca tatatgacta aaaatgaaaa aaagatagct aaatttatac
      301 taaattcacc tcaaaaggtg attaaaatga ggtctcaaga cttagcaagc ttattagata
      361 ttagtacatc atcagtcatt cgattcagca agaaaattac tgatggcggt tttcacgact
      421 tgaaaataaa tatatctaaa tatgttccta aagcatcatc aatttacaat gttgaattaa
      481 tgaataacga aagcacagaa tctttaagaa ctaaactcca tacacgtact acacgcgcac
      541 ttaatcatgc aaataacgaa ttaaatgata aaactattga tcaaatatgt cattgtttaa
      601 aacgttctga gacgattttt atatatggtt ttggcgcatc ttttgtagtt gctaccgact
      661 tataccaaaa gttatcaaga ataggtctta atattcaact tgttcaagaa acacatattt
      721 ttgctacatt attagcaact cacaattcga acgacagtgt tattcttatt actaacaatg
      781 gtacgcaaag tgaaatgcaa tcaatggtta aagttattga tgactaccat atacctataa
      841 ttacaattac tagtacaagg gataatcccg tagcacaggc atcaaatatt gttttaactt
      901 atgggaaaac tgatgaaaat gagatgcata tgggagcaac aacttcatta tttgctcaaa
      961 tgttcactat tgatatttta tattatcgtt atgttgcatt aaattatcac gcatcattag
     1021 atttcataac acaatctaaa atggctttag ataactatcg taaacattta tcaaatatta
     1081 actttaaaca ttaacagtct tatataaatt ctttaatcat gtcatataat aatttatgca
     1141 tcattttcaa agcttacatg tcttttatct tgtataacat acgcatatga ccaatgattt
     1201 gataaggcca ttcaatttct tatattaaaa acaaacgcag ttgattaacg gattcattcc
     1261 atatatcaat tgcgtttgtt attttaataa tgctattttt tattttaaag cccacagtaa
     1321 aaactaacga taatattgcc acttataagt ttttcacact acaacttcga tttaagctat
     1381 atttataaaa aaatgcgcct actcataaca ttctttggct atttaacttt aataagctag
     1441 ggccaagttt gtcataagat aagcgcacat tataatcata ctattttgct tttacattat
     1501 ttttattata tctaataaaa taataaatcg ttacaatcgt taagaagatg acaccaataa
     1561 gcactgatat tctagtttca acattaaata ccataccaac caatactaat attaagaata
     1621 agattgttaa atagttagtt actgcgccac caggcatttt gaatgggtga ccttccaatt
     1681 cttcagggtg taattttcta aaacgtaaat gactgaataa aatcataaac caaggaatca
     1741 tacctggtaa aatagacgca ctataaacgt atacgaaaat actcttcgct ccatcgatat
     1801 ataaaggtaa aattacgttt aataaagcac caattaaaat accaagagat actgctacaa
     1861 cagtgtacaa tggtacacca tttctcatga ttttagtaaa tattttcggc atttcacctt
     1921 tatgagcaag tgtataaatc atacgactcg cactgaaaat acctgagtta catcctgaca
     1981 ttgctgcagt taatacaaca aagttaatta atccagctgc aaatgtaata ccgattttag
     2041 caaatgttgc aacaaacgga cttccaatgt ctcctaattg attccaagga tacactgaaa
     2101 caataacaaa aatagcgcct aaatagaaaa ttaaaattct ccagataaca ccattcactg
     2161 ctttaacgat atttttctga ggatctttag tttcacctgc cgtaatacct atcagttcca
     2221 caccttggta tgaaccaatt acaattgata atgcaaagaa gaaaccaaca ataccatttg
     2281 gcataaatcc gccatttgtc catagattag aaatacctac cgcatggcca ccatttccaa
     2341 aaccaaagaa aataagacca aaaccagcaa taatcattaa aataattgtt acaactttaa
     2401 ttaaagcaaa ccaaaattca aattctccaa acgcttttac cgagaataaa ttcgctgcca
     2461 ttaataataa aatagcaata acaccaggaa tccaagttgg caattccggg aaccaaaact
     2521 tcatatattc tcctactgcg atgacctcac tcataccaac tacaatccat tggaatatat
     2581 tactccatgc tgtcatataa cctgctgcag gatgtatata atcacttgca aatgttgcaa
     2641 atgatcctgt tgtagggttt aaataaatca tttcccccat tgctctcatg attaaaaata
     2701 aaaagatacc cgcaattaaa tatgcaagga taactgatgg gcctgtccat ttaattgtac
     2761 ttgtcgcacc catgaaaagc cccacgccaa tcgtaccacc aagtgcaatc atttgaattt
     2821 gacgggcact caatcccctt tgtagttcat tgttctccat aacttcttac cccatctctg
     2881 attgatacta aataaatcca taaaaaaact tattgccgta attatactat aaattatcga
     2941 ttttggtaca ttaatatttg cataccatgc aatcgtttac agaactacta tagcaggctt
     3001 ttgcaacttt gcaaacaatt attttgtgat ttttctaaaa aattaaaaaa gttgctcaag
     3061 taccgttgag tcacatacaa cttcagctat tcttggtaat tatgatataa ttttaataat
     3121 attaaaaagg agtatatcaa tgttaaaaca aatactgcta cgtgctacaa aaatatcggt
     3181 actctttgct gtagcatttt tcatcataaa ttatatcggt atggaaaaac ctgacatcct
     3241 atatttagtt ggaagaacta ttattgcaac acttgctttc atactcattt gtcttacagt
     3301 atttactatt attaattctc cagagcgcaa gattaaactc ggtacgactt taccgattgc
     3361 attaattatt ggtatcattt tcggcgccat atttttaacg gtacaaatcg gtgtcatcac
     3421 tgggttaatc atcggtgtga ttgctacgtt tatttgggaa ttgattgaaa aaaataaagg
     3481 aggtcgttca tcatgacaac agcattaatt ggtgtaattg tattaatctt actaattatt
     3541 agctttgtgc caaactaccg tgctatgaaa tcagcaaaag aacaaggaag taatgcaaca
     3601 agagctacaa ttatggtagg catagatatt atcttaattg tacttatcat cgtgacgtta
     3661 ttattaaaat atatgtaact atttaaacca ccctatctca tacataaaca tgaacgttct
     3721 accatttcac atacatatgg tcgtccattt aatgaggtaa ggtggttttc tcgttaaagt
     3781 ttaccttgta atagtaaaaa atagccaatt gttgcgagta ttaagtaaat cattgaacca
     3841 acaacaaaag atcttacttg cacgcgaaat ggtgtctgtt tgttaaatat aggaccaatc
     3901 accaaatttc caacgcctaa aattactgga ccagctagaa aaaacaatat aatgtaaccc
     3961 atcatgatac gcctcctatt tcgtaaagaa acgcgctaca atttgatcgt aatcaatatc
     4021 ttttgctaca taatctacat tagaaaagtc ctgtgcgctt gtcattttat tcgtattaac
     4081 aataacatct aatccagctg caatcgcggc tgttgcacca tttacagaat cttcaatagc
     4141 caaacattgt gtcggcatat aatttaattg ttgtactgca gataaatata attcagggtc
     4201 aggtttaact tgttcaacat cttctctacc aacaactacc tctatataat cgtctagacc
     4261 taatgcttta aaggtgggca ttatttccgc tttcacacta cttgtggcaa ttgccatagg
     4321 aatgtgacgt tgctttaaaa atgccatcaa tgatttaatc gtatcaatca tcggtaatgt
     4381 tgtactagta agatgatgtt cttcataaag tttatcttta ttttccgaac caatcgcttt
     4441 aattaaatgg tcatgcaaat ctgtagctgc tcctccaata gaagaacgat aaaaatcaat
     4501 gcttatagga tcggcattat gcatctctaa atgtttatta ataacattaa ataaatgttg
     4561 ttccgtatct attattgttc catcgaaatc aaatataact gctctataca tatatttact
     4621 cctctatttt ttctctttat caaagcttaa catagtccga tgaccacttc attctattag
     4681 tgcaattata acttttttga ccaatacttt gctaaaatag gccctgttat attatgaatg
     4741 aaactaaaca ctgcgcctgg tactgctgca attggattaa aatgcaatgc tgctaatgac
     4801 acagctaaac cagagttctg cattccaact tcaatactga ctgctttttg atcttgtcga
     4861 tctaatttta aaagacgagc taaccaatat ccaatcgtat accctaatac gttatgtaaa
     4921 ataactacta aaaatattaa taatcctgta gttaagattt ggtgcttact gccacctaca
     4981 actattgcta aaattaatga aatagcaaca actgatataa ttggcaaagc tgtagaagct
     5041 ttttcagcaa ttttacgatt aataatttgc aaaacaatac ctaaagcaat tggaattaat
     5101 acaacttgaa caactgacca caacatactc acgaaagata cttccaacca ttcatttgca
     5161 aatagatata ttaacgcagg tgtaacgaat ggcgctagca acgtagagac cgtcgtaata
     5221 gaaacagaaa gtgctacgtt agctttggct aaataactca ttacattact tgatgtccca
     5281 cccggacaac atccgactaa tattacgcca acagcaatat caggtggtaa atgaaaagac
     5341 tttgcaatta taaatgctaa tgtgggcata atactgaatt gtagacagac accaataatt
     5401 actgctctag gtgctttaaa gaccattttg aaatcattag gtgtaattgt taatcccata
     5461 cctaacataa caataccaag taaataaggt acccatttac ctaatgtcgc aacatgttgt
     5521 gggaaaataa atccaataac agctgctact aacatccata ccaaaaaact cttcgtggca
     5581 aatatactca atttttgaaa cattgttaac acatcctcgt cgataatatt gaaaaatatt
     5641 accataagac cagtaaggtt acaattactt ttctgaaaat taagattatt tagtcattct
     5701 cttcttattg caaatatttt atcactgata aacaattatt ttaaacttca ttcaaaagtc
     5761 ttagtgaata aagaaaaaaa gcgtggtaaa atcaacttga acaaaattaa gtcaccttta
     5821 taaatattaa acgtgtattt aatgccatgt attcctcggg aggtcctcac atgttaattg
     5881 ataaattcga aacatacatc atcaacatcg ccggtttaaa tgaccgtact actagaaaaa
     5941 aattaagtaa gttatgcaaa agtgtacaat tttgtgatgc gttgcaattc tcaataaaca
     6001 aacagtttaa tcagtatgta ttagaaatta gcttaccaaa gcaacaactt ccatacttca
     6061 tttcattttt aagttttcat cagtatagta ttttccaagt actttcacca aagaaaataa
     6121 atgaattact tgattctgat aatttatatc aatctgcaaa gcgtttcgat atcaatattg
     6181 atggcttgca agatgctttc attaaagaca aagtcatcga tattatgaat atgttccaaa
     6241 atcacactga tatcacttat acgttgaata agtctcatgc acatattata tgtacgccag
     6301 aaatatttgc taaattgtta catacgattg caactagaaa tatcgacatt ctatctgcca
     6361 attatagatc gtcatctatg agcaaagctc gtatatcata ataaaagaca catctatacg
     6421 atgatcattt acaatgattg cgtatagtga tgtgtctttt tttgtattat ttatcttcgg
     6481 acgattgatc ctcaaccatt tgttcaaatg cttcacgaac ttgtggtact gtcataccta
     6541 ctacaacttg gatactctta ccactcttta ctaatccgtg tgccatttgt tgatgcgtga
     6601 aatattccgt atctgcaact ttactttcat cataaacagt taagcgtaat cttgttgtac
     6661 agttagtaac atctttgata ttttctttgc cgcctaaacc gtcgaggtaa tatgcagctt
     6721 tgtcttcgta ttcattacca gatgaagcac gtttagaatc tacgctatca ccttttttat
     6781 ttttgtaacc ttgcttagaa aataatttaa cttcctcttc tgttttctta cgtccaggta
     6841 atggaatatc aaatttcaaa attaagaatc tgaataagaa gaagtaaatc gctacaaata
     6901 tcagaccgat aataacttgt gcaacataag taccccaatg ttctttacct aatggcaacc
     6961 agtttgttga aataaagtct aacaagccac cacccatgtt acctacaaca ccaaatgcgt
     7021 acattaaagt atccattgat gctgctaaaa ctgcgtgtaa aacaaataag tatggtgcga
     7081 tgaataagaa tgtaaactct aatggctcag taatacctac aacgactgct gttaatgtgg
     7141 caggaataag tagtgccgcc acctttttac gattttcttt aggtgtcgta gcatacattg
     7201 ctaaagcaat acctaaacat ccaaatactt tcccatttcc ttgaagcata aagccataag
     7261 ggaattgctc ttttaatggc ttagtactct tagcaaactc atttaaatgt tgtaaccatt
     7321 cagctttcaa gccgtgattg actactactg gtccaacttc aattggtgca taaataaagt
     7381 gatgtaatcc tgttggtatt aatacacgtt ctaagaagtg atacaaccat acaccgatat
     7441 agcctgaagc aatgataaag tgttgcattg aaccaatacc gtgctgaaca gttggccaaa
     7501 taacacaagt aattgctgct aatggtaaca tcacaaaaaa tgaaatcgta actacaaatg
     7561 ttaaaccttg gaatacacca accatttctg gtaatcgttt actatagtat ctattgtgaa
     7621 tccaagtaat aatagctgaa ataataatac cacctagaat attcgtatcc aatgtggcaa
     7681 tacctgcaat tgattttaaa ccaggtacat tttcaacgcc tttttctaaa ttagcgccaa
     7741 acgtatgtgg ccattgtgtt aaaatggcat ttataaatgt attaaacatt aagtaaccca
     7801 tcaatgctgc aagtgctgca tgacctggtg cttttttagc taaagaaagt ggtagaccta
     7861 caacaaatac aacttccata tgcgtaaaaa taacccagcc accagattct attactgacc
     7921 aaaatttaaa ccaaaacgta tgttgatctg ctaaactccc catgattgta ggatttttaa
     7981 ataatgtcgc aaagccaagc actatcccaa agaaagcgaa cattaatacc ggtacaatca
     8041 ttgcactacc gaaacgcttt atcgcattca tcttctattc cctccatatc atctttccta
     8101 acaatacatc taattagatt catttataaa tagatgtctt actatttaaa tataatatat
     8161 agtaaacgct tacacaccta caacgacatt gacgtatttt gaaagtattt tgtataatca
     8221 gattatcttt tcatatagtg aaaatttttt cacgacctta tatatgacat cgttgtattt
     8281 gtaatacatt cgttttaaac gcataatcaa acctatatca atacacaaat atatataatg
     8341 acatacaaga ttttaatgta ataacgatct attacacatt tattttcaag gaggttgaat
     8401 atgtttttag atgaacacat taatcgaaac tttgataaac ttaatgataa tgatttacat
     8461 atcgctcact ttatcaatac acatatagat gaatgtaaaa atatgaaaat acaagattta
     8521 gcgcaattca cacatgcctc gaatgcaacc attcacagat ttacacgcaa attaggtttt
     8581 gacggttata gtgattttaa atcgtacctt aaatttgaaa gtaataagac acaccaatta
     8641 ccttctaact ctatcgatag tttcaaacaa gaaatcgata gtacattcag ttatttagaa
     8701 cgcgttgatt accagcttct tactaaaaaa atgcatacag cttcaacaat atatttgtat
     8761 ggtacagggc gtgcacagtt aaacgttgct tcagaggcac agcgtatatt gttaacgtta
     8821 cataatcatg tcattgtact tcatgatgaa catgaactta aaatgatttt gaacaagagt
     8881 gaacctaatg atttattctt tattatttca ttatcaggtg aaacacatga attattagaa
     8941 gtaacaaact tgcttcaact tagacaaaaa tactttattt ctgttactac aatgaaagat
     9001 aatacccttg ctcaaaaagc aaattacaac gtctatgtat ccagtaacac attctactta
     9061 actgatggta cagactattc tagcttcatc agttatcata tattctttga aacacttgta
     9121 agaaaatata atgagtattt actacataat gatatttcaa attagaaaaa aggaagttat
     9181 gatttaatgg ccttgagcct atcataactt ccttttatca ttttattgtt gtgttgatgt
     9241 ttcgataacg tggtacatct tatcaaacat caattcgaaa ccatgcacca tggcatcatg
     9301 atattctttt ttcttttgct tgtattctaa attagtaaat cgtctttctt tttcaactaa
     9361 tgaacgataa taaaatagca tttgggtgcc acctgtttca cgttcaaaaa attctacctc
     9421 aatgacatct tgcgtttcac ttagtccagg cataccgata gtcatcttaa cgtattcatc
     9481 cataactaaa gattcataaa tgccttcaat cacatttact ttgccattac gttgttgatc
     9541 tacaatacga tatttaccgc cttctttaac gtccgcttca atctctttat tcgttctggc
     9601 tgatgtcata aaccattgtt tcaacaaatc tttctttgtc caagcttcgt atactaactc
     9661 tggagaaaat ttataaagct tttcaatttc aacttcgaca tgttcattct ctacattaaa
     9721 ttttgccact gttgtccacc cactttcgct cttactttta ttttaacgta tttttgctca
     9781 gttccaaaca tagatgatca tcatttttaa aagattagcg ttatacggtg agtacaacat
     9841 gatctgttaa tataacaagc caccttactt ggctacatcg atatattgtt aagcattaat
     9901 gtttcatttc ttgactagtg ttctttttta gctttggaaa attaaataaa atcgcaataa
     9961 gtccgcatac acctaataat ataggataaa tgctgtatgg gaataacatt aacggtgaaa
    10021 taccagctac accagccgct gaaatgactt gcgggctata tggtaataaa ccttggaagc
    10081 agcctccaaa tatatcaaga atacttgctg atttccttga atctacatca tattcatctg
    10141 caatattttt agctaaagga cctgacataa taatagagat ggtgttgttt gccgtggcaa
    10201 tatctgcgac acttaccaaa ctagcaattc ctaattctgc gccacgcttt gatttcactt
    10261 tagagcgaac aaattgcaac aaccattcaa taccaccatt gtgttgaata ataccgacta
    10321 aaccaccaat tagcaacgca atcatagcaa tatcttccat gcttataata cctttggaca
    10381 ctgcatctag tagccccatc caaccgaatg aaccatctat gagaccaatg attccggcta
    10441 ataatgttcc gccaatcaat acgataatga catttacacc taataatgct aataccaata
    10501 ctaagatata cggtacaact ttaattagat tataatcata gtttttagca tgatttaaag
    10561 aaatgccatt cgttaagaaa tacagaataa taatcgttaa aatagcacct ggcaatacaa
    10621 ttttaaagtt tactctgaat ttatctttca ttttcgtatg ttgtgttcta accgcagcaa
    10681 ttgttgtatc tgaaatcatt gatagattat cgccgaacat tgcacctcca acaactgtag
    10741 ccattgctag cgcagttggt acatctgtcg cttgtgcaaa tccgtaacca acaggtgcta
    10801 ttgcagcaac agttcctaca gacgtcccca tagatataga aacaaacata caaatcacaa
    10861 acaatcctac aataattaaa ttttctggga ttaatgatag tcctaaatta actgtcgact
    10921 ttacgccacc cattttttca gctgtatttg aaaatgcacc tgctaaaata aaaatcaaca
    10981 tcattaaaac aatgtttgaa tggcctgcac ctttcgtgaa gacctcaact tttttagcaa
    11041 atgattcttt tcgattcatt aataacgcca caattaccgt tatcgtaatt gcaacattta
    11101 atggcattga agtaaaatca cctgtgataa tacctacgcc taaaaacaac gccacaaata
    11161 ataacaaggg gaataatgcc caagcattgc tctttttatg tacttccatc ctttttacct
    11221 gctttccaat taaaaatacc tctttctcac aaacgatgaa gaaagaggtt ttcatgtgct
    11281 ttacctgctt atcttcaaac cattacggtt actggaattg gcacattcga gatgttgccg
    11341 aggcttcata gggccagtcc ctccacctct ctagataagt gatgcttatt tacgtttacg
    11401 ttacaagata atccttagta cgtcaatcat aaattaatca ggagtcgtat aatatttttc
    11461 ataaacaatc attgctactg taataataat caaaacaata atgctaataa caagtaaaag
    11521 ccaccattta agcattaatg caataaaaat gaacacgata gacacactta ctaatattaa
    11581 tgatatgact ttaaattgct gaacacgttg cttggagatg actttcaact gtttgtttga
    11641 tagacgcgta ttttttatac tgattcccag tatattttct aatatttgaa ccaatacgat
    11701 acttattgca aatataataa ttggtaaaac atcatagctc cctatagtta atgtataaat
    11761 tacaaatcca atgtaaagta accctgagac aaaggataaa aagtatgcga cgtatttgtt
    11821 aaacttaatg atatgctttt taacgttttg atgtgtaaac catacattcg aaacgatcgc
    11881 aactgctaca aataatgtga atactatata taatggtaat ttttgttcag gaaaaacagt
    11941 cgctattcca aaagctaatg ctaaaatcaa aaataatata gctctagata ctattaatgc
    12001 cataataaca acccctttgt ttaatatcga gtttgcaaat ttacgtttat cagcgtttct
    12061 atgatcagta cttctacggg tagcgtttct atgtaattta catcatctta acatataaat
    12121 acttcgctat ttaattgaaa acatatccta ttattctttg tccgttctga cgtttaatat
    12181 ctagccttag gcatttcact tgttaatgaa tttaactttc ttccactaac cgtccctaaa
    12241 cccaatcccg caacagtttt taactttttc gttgttgtcc tgacatcctc attaagaaag
    12301 tttattctgc ttaaaactta taatccacac cctgagcaaa cgctccttat gacagagtat
    12361 taaaataagc cgataaagat acacaccttt accgactatt taaaatacac ttcaccaatt
    12421 cattttaatt taatggattg aagtaactaa attaatatta tgttgttcaa ttaaaagctt
    12481 catacaaacc taatctattt gcactccacc gctaacaccg aacacttgtc cggttgtata
    12541 acttgattct tctgacgcta atagcacata agttccacat aactcaacag gttgacctgc
    12601 acgacctaaa ggtgtttttt gaccaaatgt tgggatttta ctttgaggtt gtccaccaga
    12661 aatttgtaat ggtgaccaga atggaccagg cgctacacag ttcactctaa ttccttttgg
    12721 tcctaattct tctgaaaaac ttttagttaa tgaaataatt gctgcttttg aagcggcata
    12781 atcatgaaga ataggactag gattataacc ttgtacagat gatgtcgttg taattgacgc
    12841 acccggtttt aaatattcca atgctttttg aactgtccaa aatagcggat agacattcgt
    12901 ttcaaatgtt tctgtaaatg cctcagttgt aaatccatga atatcatcat gatactgttg
    12961 atgtccagca actaaagtaa cattatctaa gccacctaat tgttgatatg cttgttcaac
    13021 aaggtcatag ttgaactgtt catctcttat atcaccagga attaacactg ccttttgacc
    13081 actttcttca atcacttggc gtacttcttg tgcatcttgt tcttcactcg gaagatagtt
    13141 aatcgctaca tctgcacctt ctttagcata cgcaattgct gctgcacgcc ctattgctga
    13201 gtcaccacct gtgactaata ttttatagcc ttgtaagcgt tgatgacctt ggtaagacgt
    13261 ttcgccacaa tcgggtgctg gcgtcatttc agattgtaaa cccggtacct cttgttcttg
    13321 tttttcataa tccgttgttt taaattttgt tctaggatct tgagctgcca tttttttaca
    13381 tctccttatt cgcttaatgg ttattattta cccaatcttc ctaggaactt aatcatgatt
    13441 acactaaaaa ttactttctt ctttataaaa acaagctcga attattcatg caatagtctc
    13501 tttacaaatt caacaaaata ctcaggtact ttttccagaa tcctttcatc cggtttatat
    13561 tgaggatgat gtaaatcata ttcactatga gaaccaatta acgcaaatac acttggaaaa
    13621 tgttgactat aacctgaaaa atcttctcca atcgtaagcg gctgttccat catttccacc
    13681 ttatatccaa catgttgggc tactgcaatt gctttatgcg tcaatgcctc atcattcatc
    13741 acagcgccag gtaaatgcgt ataatttaaa ttgattttca tattatatgc ttgagccaat
    13801 ccgtccgcaa tatcttgtaa tcgtgtttct acaagctttc gtaccacagg atcaaaacta
    13861 cgcactgtgc cttgtacata cgcatgatca gcaatgacat tccaagtatt accacatgat
    13921 atttgtccaa ttgttactac cgcttcatca aacgcagata gatttctact aactatggat
    13981 tgaatactat taatcaattg cgccaacaca ataactggat cgttgcattg ttctggcttt
    14041 gcagcatgac cacccacgcc tttaatatga aactcaaaac gatctactgc tgatgtaatt
    14101 gcccctgttt tgattgcaaa tgtacctacc gaacgcgatg ggtcattatg aaaacccaat
    14161 actgcttgta catcttttaa tgcatgtgtt tcaataattt taaaagcgcc atgtcctagt
    14221 tcttctgctg attgaaaaat gaatttaaca cgcccagtaa gagtgccctc aatttctttt
    14281 aattttacag ctgtagccaa aatactagcc atgtgaatat catgaccaca cgcatgcata
    14341 acaccttcat tttcagattt aaaatcctgt tcaacaagct cttgaatcgg taaagcatca
    14401 atgtcagccc gaacagctat acaactcaga ccttgtccca cttcagcaac aagcccagtc
    14461 gcaagtggta agtctaatat tctaatatga tgttctgtta aaatatcttt aattttttgt
    14521 gtagtcttaa attctttatc ggatagttct ggaaattgat gaaaatacct tctccaggta
    14581 acagcttgat cttttaatcc catcggtcat tccccttcct taagtcaatg atatgttgtc
    14641 taccctacga tgatcatctt tgactattaa acgatgattt cacaacaatg tactcttgtt
    14701 aattgctttc gttaatgata gacagttgtt taataatatc gtaacactgt tgtcaaacta
    14761 ttctaacttt tataattgag actctataca aaaacgtgtt ctcgaatata cttgttttta
    14821 caaaccacaa aaagctctaa acattagttt aaaccaatgc ttagagcttt ctaattattt
    14881 tatgctttaa aagatactgt gttatctacg atgaccttac cgtctttaat aactttttct
    14941 gcgtgattga taccaaaatg atatggaata tattcatgat ttggtgcatc ccaaattact
    15001 aaattagcct tatcacctgt gttaattgta cccgcgttaa tgtctattgc tttagcagca
    15061 ttgaccgtaa cagcattcca aacttcatta ggtgatagct ttaatttcaa ggctgcaatc
    15121 gccataacaa gttgtaagtt gtttgtgaca ctactaccag ggttataatc agttgctaat
    15181 gcaatcgcac cgttattgtc aagcatgcct cttgcatctg cataatcttc tttacctaaa
    15241 tagaacgtcg ttgcaggtaa gaggacagct acagtatcac tatttcgcaa cttttctttt
    15301 cctttatcac tagaagctac taagtggtct gctgatattg cttgttcatc aattgctaat
    15361 tccagtccgc ctaacggatc aatttcatcc gcatgtattt tcactttaaa acctgcttct
    15421 ttggcttttt gcatataatg ttgcgattgt tctattgtaa atacacctgt ttcacagaaa
    15481 atatccgcaa agtctgcata ttgttttact tccggaagta acgcaatcat ttcttctaaa
    15541 aatgcctcat ttgaacttgc ctctttaggt acagcatgag gccctaggaa agtatgtttc
    15601 atgtctaaat catatttctc agctaaacga ttagacactt tcaattgctt cagttcattt
    15661 tctctatcta atccataacc actcttactt tcaactgcaa gcacgccgtg tttaatcata
    15721 gtaagcaaat catgctctgc ttttttaaac aagtcatctt cggatgtttc tctagtagca
    15781 ttaacggtag ataatatgcc accacccatt tctaatattt caaggtaaga cttaccttga
    15841 cgttttaatg acatctcatg ttctcgagat ccaccaaatg ttaaatgggt atgtgcatct
    15901 actaatgctg gggacactac cttcccacta gcatcaatcg tctcagtcgc atcgtagtca
    15961 tctgtatgtg ttccagcata tacaattttg ccatctttaa tgacaactgt accatttttc
    16021 acaacattta attcatctaa ttccttaccc ttcaaaggtt tatctgttga tctcggtaaa
    16081 attaattctg ctatatgatt aattattaaa tcattcatta cttatcacct gctttatcaa
    16141 tcattggaat atgaatacct ttttctttag ctgtttgaat agcgatgtca tagccagcat
    16201 caacatgtcg ggcaacaccc atacctgggt cagtcgtcaa tacacgttcc aatcttcttt
    16261 cagcacgctc tgatccatct gctacaacaa ccatacccgc atgaagtgaa tatcccatgc
    16321 caacaccgcc accgtgatgg aatgaaatcc atgaaccacc tgcagctgtg ttaatgagtg
    16381 cattcaatac agcccaatca ccaaccgcgt cactaccatc tttcatactt tctgtttcac
    16441 ggttaggact agcaactgaa ccagcatcta aatggtctcg tccaataaca attggtgctg
    16501 aaatttcacc gtcacgtaca agacgattta aagctaagcc cattttcgct ctttctccat
    16561 agcctaacca agcaatacgt gatggtagtc cttgatatga aattttttct tcagctaaat
    16621 caagccatct taataacttt tcattttctg ggaaaagttt gcgcatttct tcatccgcac
    16681 gctcgatatc ttttggatca ccactcaacg cagcaaagcg gaatggccct ttaccttcac
    16741 agaataatgg tctaatgtaa gctggtacaa agcctgggaa gtcaaaagca tttttcactc
    16801 cgttattgaa ggctacttga cgaatattgt taccataatc aaatgctaca gcgccacgtt
    16861 tttggaattc aagcattaat tcaacatgct ttgccattga agcttgtgac agttcaacat
    16921 attttttcgg atctttttca cgcaatactt tcgcttcttc tacagagtat ccttgtggca
    16981 catatccatt tagcggatca tgtgcacttg tttggtcagt aataatgtca attttaaatc
    17041 ctttttctag aatcgcttga tggatgtcta cagcatttcc aactaacccg attgataatc
    17101 cttctccacg ttctttcgcc tcttctgcta attttaatgc ttcatctaaa tcagctgttt
    17161 taacatcaca gtatttcgta tcaattcgct tatcaacacg tgtttcatca acatccacgc
    17221 aaattgctac cccatgattc atagtaattg ctaacggttg cgcaccaccc ataccaccta
    17281 aacctgctgt cagtgtaaca gtgcctgcta aatctccatt aaagtgttga ttacctagct
    17341 cggcaaatgt ctcataagta ccttgcacaa taccttgaga accaatatat atccaactac
    17401 cggctgtcat ctgtccatac atgattaaac cttttttatc taattcatta aaatgatccc
    17461 agtttgccca ttcaggcact aatactgaat ttgaaattaa tacacgtggc gcttcttcat
    17521 gtgttttaaa tacagcaact ggctttcctg attgtactaa cattgtctca tctgattcta
    17581 attctcgtaa cgttttctct attgcttcaa aagcttccca attacgtgct gcttttccaa
    17641 taccaccata aacaactaaa tcttctggtc tttcagcaac ttctgggtct aaattgttgt
    17701 ataacattct aagtactgct tcttgttccc aacctttaca ctcaatactc aaaccttttt
    17761 ttgcttgaat ttttctcata aaattcgctc ctgttctttt aagaagttaa ttccactaaa
    17821 tttaaaacgc ttacattatt atcttcaata ttcattatag tatgttaaaa tatagccaac
    17881 aaatataaat aaactaatta tccatagctt gaatctataa ataaaaggag caaaacacat
    17941 gaaaattatt cagttagaat acttcttggc tatcgtgaaa tataatagtt ttactaaagc
    18001 tgcacaattt ttacatatta gccagccatc tttaactgct acgattaaaa aaatggaagc
    18061 agatttaggt tatgacttat ttacacgttc aacaaaagac atcaagatta ccgaaaaagg
    18121 aatacagttt tatcgttatg cgagcgaatt agttcaacaa tatcgatcca cgatggaaaa
    18181 aatgtatgat ttaagcgtta catcagaacc aaggataaaa attgggactc ttgaatctac
    18241 gaatcaatgg attgcgaatt taattcgaaa gcaccattcc gactaccctg aacagcaata
    18301 tcgtttatat gaaatacatg ataaacatca atctatagag caattactga attttaatat
    18361 tcatttagct ataacaaatg aaaaaataac ccacgaagat ataagatcca ttcctttata
    18421 tgaggaatct tacattttat tagcacccaa ggaaacattt aaaaatcaaa attgggtaga
    18481 tgttgaaaat ttgccactca tattaccaaa caaaaattct caagtgcgca aacacttaga
    18541 tgactatttt aatagaagaa atattcgtcc aaatgtcgtt gtagaaacag atcgattcga
    18601 atcagcagtt ggatttgttc atctcggctt aggttacgct atcattccga gattttatta
    18661 ccaatcattt cacacgtcta atttagaata taaaaaaatt cgtccaaact taggccgaaa
    18721 aatttatatc aattaccata aaaaacgcaa acactccgaa caagtacata cattcgtaca
    18781 acaatgccaa gattatttat atggactttt agaggctctt taacttaagt tattagagcc
    18841 tcttatgcag ttgcgcagat catcgtataa aaattaatga cgtcatttca aaaatcgata
    18901 caaaaataat ttattataaa aattctaaga aagaagtgaa gcagatgtta aaatctatta
    18961 atcatatatg cttttcagtc agaaatttaa acgattcaat acatttttat agagatattt
    19021 tacttgggaa attgctattg actggtaaaa aaactgctta ttttgagctt gcaggcctat
    19081 ggattgcttt aaatgaagaa aaagatatac cacgtaatga aattcacttt tcatatacac
    19141 atatagcttt cactatagat gacagcgaat ttaaatattg gcatcagagg ttaaaagata
    19201 ataacgtgaa tattttagaa ggaagagtta gagatattag agatagacaa tcaatttact
    19261 ttaccgaccc tgatggtcat aagctagaat tacatactgg cacacttgag aacagattaa
    19321 attattataa agaggctaaa ccacatatga cattttacaa ataaggtgtc attataaaaa
    19381 ggcctcttga actccgttaa aattttaatt aattattata taataagaga acttttcaaa
    19441 caatacagtt gttatttttg ctatttcaac aaacataaat aa
//
LOCUS       DAKTIO010000034        19471 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.034, whole genome shotgun sequence.
ACCESSION   DAKTIO010000034 DAKTIO010000000
VERSION     DAKTIO010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19471
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.034"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..157
                     /locus_tag="O0293_000910"
     CDS             <1..157
                     /locus_tag="O0293_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCY0279804.1"
                     /translation="HYIEVWGKFTPRGGISIDPYTNYGRPNSKYEKMAEHRLMNHDLY
                     PEKIDNR"
     gene            498..2003
                     /locus_tag="O0293_000911"
     CDS             498..2003
                     /locus_tag="O0293_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HCY0279805.1"
                     /translation="MTQQNSHGNQIQDIPQTGFFGHPRGLGVLFFVEFWERFSYYGMR
                     ALLIFYMYFAVTDNGLGIDKTTAMSIMSVYGSLIYMTSIPGGWIADRITGTRGATLLG
                     AVFIIIGHICLSLPFALIGLFTSMFFIIIGSGLMKPNISNIVGRLYPENDRRMDAGFV
                     IFYMSVNMGALLSPIILQHFVNVKNFHGGFLIAAVGMALGLVWYVLFNRKNLGSVGMK
                     PTNPLTPAEKKKYGLIIGSVVLAIVLIIVIGALTNSLSFNLVSNTVLVLGIALPIIYF
                     TLIIRSKDVTDTERSRVKAFIPLFILGMVFWAIQEQGSNVLNIYGIEHSDMKLNLFGW
                     KTNFGEAIFQSINPLFILLLAPIISLLWQKLGTKQPSLPVKFAIGTFLAGASYILIGI
                     VGYASGSSNFSVNWVILSYIICVIGELCLSPTGNSAAVKLAPKAFNAQMMSIWYLTNA
                     SAQAINGTLVKLIEPLGQTNYFIFLGVVAIIVTTIVLAISPLIIKAMKGIR"
     gene            complement(2081..2182)
                     /locus_tag="O0293_000912"
     CDS             complement(2081..2182)
                     /locus_tag="O0293_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279806.1"
                     /translation="MVINEIDFYRVGRENIVIAVYNEHIKFLFILIS"
     gene            2273..3190
                     /locus_tag="O0293_000913"
     CDS             2273..3190
                     /locus_tag="O0293_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCY0279807.1"
                     /translation="MENKYTHGVLFYHEHSGLKNINQGIGEVTTALSSICKHLSIQLS
                     ENEGDIIKYCQEIKTKNYAKDVDILFILGGDGTVNELINGVMTHDLQLPIGILPGGTF
                     NDFTKTLNIAPNHKQASEQMISAQVGTYDVIKINNQYALNFVGLGLIVQNAENVQDGS
                     KDIFGKLSYIGSTVKTLLNPTQFNYQLSIDDKTYSGETTMILTANGPFIGGSRIPLTD
                     LSPQDGELNTFIFNEQSFSILNDIFKKRDSMNWNEITQGIEHIPGKKISLTTDPAMKV
                     DIDGEISLETPIDIEVIPNAIQLLTVNDL"
     gene            complement(3814..4356)
                     /locus_tag="O0293_000914"
     CDS             complement(3814..4356)
                     /locus_tag="O0293_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'(3')-deoxyribonucleotidase"
                     /protein_id="HCY0279808.1"
                     /translation="MTRKSIAIDMDEVLADTLGEIIDAVNFRADLGIKMEALNGQKLK
                     HVIPEHDGLITEVLRESGFFRHLKVMPYAQEVVKKLTEHYDVYIATAAMDVPTSFSDK
                     YEWLLEFFPFLDPQHFVFCGRKNIVKADYLIDDNPRQLEIFTGTPIMFTAVHNINDDR
                     FERVNSWKDVEQYFLDNIEK"
     gene            complement(4707..5765)
                     /gene="hisC"
                     /locus_tag="O0293_000915"
     CDS             complement(4707..5765)
                     /gene="hisC"
                     /locus_tag="O0293_000915"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCY0279809.1"
                     /translation="MKEQLNQLSAYQPGLSPRALKEKYGIEGDLYKLASNENLYGPSP
                     KVKEAISAHLDELYYYPETGSPTLKAAISKHLNVDQSRILFGAGLDEVILMISRAVLT
                     PGDTIVTSEATFGQYYHNAIVESANVIQVPLKDGGFDLEGILKEVNEDTSLVWLCNPN
                     NPTGTYFNHESLDSFLSQVPPHVPVIIDEAYFEFVTAEDYPDTLALQQKYDNAFLLRT
                     FSKAYGLAGLRVGYVVASEHAIEKWNIIRPPFNVTRISEYAAVAALEDQQYLKEVTHK
                     NSVERERFYQLPQSEYFLPSQTNFIFVKTKRVNELYEALLNVGCITRPFPTGVRITIG
                     FKEQNDKMLEVLSNFKYE"
     gene            complement(6005..7519)
                     /locus_tag="O0293_000916"
     CDS             complement(6005..7519)
                     /locus_tag="O0293_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HCY0279810.1"
                     /translation="MTNFFDILSERKGQLFSTMIEHIQISFIALLIATAIAVPLGILL
                     TKTKTISEIVMNIAAILQTIPSLALLGLMIPLFGIGRVPAIIALVVYALLPILRNTYT
                     GIKEVDPSLIEAAKGIGMKPFRRLTKVELPIAMPVIMAGVRTAMVLIIGTATLAALIG
                     AGGLGDLILLGIDRNNASLILLGAIPAALLAIIFDLILRFMAKLSYKKLLMTLGVIVM
                     IIILAIAIPMFAQKGDKITLAGKLGSEPSIITNMYKILIEEETKNTVEVKDGMGKTAF
                     LFNALKSDDIDGYLEFTGTVLGELTKEPLKSKEEKKVYEQAKQSLEKKYQMTMLKPMK
                     YNNTYALAVKRDFAKQHNIRTIGDLNKVKDQLKPGFTLEFNDRPDGYKAVQKAYNLNL
                     DNIRTMEPKLRYQAINKGNINLIDAYSTDAELKQYDMVVLKDDKHVFPPYQGAPLFKE
                     SFLKKHPEIKKPLNKLENKISDEDMQMMNYKVTVKNEDPYTVAKDYLKAKGLIK"
     gene            complement(7512..8489)
                     /locus_tag="O0293_000917"
     CDS             complement(7512..8489)
                     /locus_tag="O0293_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0279811.1"
                     /translation="MIKFKNVTKRYGKHVAVDNISFNINEGEFFVLIGPSGCGKTTTL
                     KMINRLIHLSEGYIYFKDKPISDYPVYEMRWDIGYVLQQIALFPHMTIKENIAQVPQM
                     KKWKEKDIDKRVDELLEMVGLEPEKYKNRKPDELSGGQRQRVGVIRALAADPPVILMD
                     EPFSALDPISREKLQDDLIELQTKIKKTIIFVTHDIQEAMKLGDKICLLNEGHIEQID
                     TPEGFKNNPQSEFVKQFMGSHLEDDAPCVEENAIIRDLDIMKPIDEVTSMSAYPIVYD
                     NQPIEVLYQLLSESERVIVMQEDSVGQYVIDRKDIFKYLSQKKEVAQHD"
     gene            complement(8711..10492)
                     /gene="recQ"
                     /locus_tag="O0293_000918"
     CDS             complement(8711..10492)
                     /gene="recQ"
                     /locus_tag="O0293_000918"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HCY0279812.1"
                     /translation="MMQQTLSHYFGYETFRPGQEEIISKVLDHRNVLGVLPTGGGKSI
                     CYQVPGLLLGGTTIVISPLISLMKDQVDQLKAMGIQAAFLNSSLTQKEQQRIEKALSN
                     GEIQFLYVAPERFENRYFLNMLQRIKIHLVAFDEAHCISKWGHDFRPSYQNVISKVFT
                     LPQDFTIIALTATATVEVQQDIREKLNIAQTDQIKTSTKRRNLIFKVNPTYQRQKFIL
                     DYIKTHDEDAGIIYCSTRKQVEELQEALESQKIESVIYHAGLSNKEREEAQNDFLFDR
                     VKVVVATNAFGMGIDKSNVRFVIHYNMPGDLESYYQEAGRAGRDGLKSECILLFSERD
                     INLHEYFITVSQADDDYKDKMGEKLTKMIQYTKTKKCLEATIVHYFEPNEKLEECEQC
                     SNCVQQDKSYNMTQEAKMIISCIARMKQQESYSVIIQVLRGESTDYIKYKGYDQISTH
                     GLMKGYTTSELSHLIDELRFKGFLNENDEILMCDTSIKKLLSNEVEVFTTPFKQKATE
                     KVFINTVEGVDRVLFSQLVEVRKKLSDKLTIAPVSIFSDYTLEEFAKRKPASKQDMIN
                     IDGVGSYKLKHYCPAFLETIQNYKAKV"
     gene            complement(10504..12387)
                     /locus_tag="O0293_000919"
     CDS             complement(10504..12387)
                     /locus_tag="O0293_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCY0279813.1"
                     /translation="MGMEAYKIEHLNKSYADKTIFDNLDLSISEGEKIGLVGINGTGK
                     STLLKVIGGIDDDFTANVMHPNQYRIRYSSQKQDLNEDMTVFDAVLSSDTTTLRIIKQ
                     YEQAVQAYADDQSDKLFKRMMDAQDAMDQHDAWDYNAEIKTILSKLGIHDTTKYIKEL
                     SGGQQKRVVLAKTLIEQPDLLLLDEPTNHLDFESISWLINYVKQYPHTVLFVTHDRYF
                     LNEVSTRIIELNRGKLASYPGNYESYIEMRAEREVTLQKQQQKQRALYKEELAWMRAG
                     AKARTTKQQARINRFNDLENEVNQQYKDDKGELNLAYSRLGKQVFELEDLSKAINDKV
                     LIEHLTEIIQKGERIGVVGPNGAGKTTLLNILSGEDQQFEGKLKTGQTVKVAYFKQTD
                     ETLDRDIRMIDYLREENEIAKEKDGTSVSITQLLERFLFPSATHGKKVYKLSGGEQKR
                     LYLLRLLVHQPNVLLLDEPTNDLDTETLTILEDYIHTFGGTVITVSHDRYFLNKVAQS
                     YWFIHDGQMEKIIGTFEDYESYKKSLDKNKSTLKQQSKSSTTVRKKNGLSYKEKLEYE
                     QLMKRIEQAEVRMEEIDVLMIEASADYGKIKELNEEKEQLEIQYDLDITRWSELEEIK
                     EQQ"
     gene            complement(12658..14598)
                     /gene="ltaS"
                     /locus_tag="O0293_000920"
     CDS             complement(12658..14598)
                     /gene="ltaS"
                     /locus_tag="O0293_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyglycerol-phosphate lipoteichoic acid
                     synthase LtaS"
                     /protein_id="HCY0279814.1"
                     /translation="MSSQKKKISLFAFFLLTVITITLKTYFSYYVDFSLGVKGLVQNL
                     ILLMNPYSLVALVLSVFLFFKGKKAFWFMFIGGFLLTFLLYANVVYFRFFSDFLTFST
                     LNQVGNVESMGGAVSASFKWYDFVYFIDTLVYLFILIFKTKWLDTKAFSKKFVPVVMA
                     ASVALFFLNLAFAETDRPELLTRTFDHKYLVKYLGPYNFTVYDGVKTIENNQQKALAS
                     EDDLTKVLNYTKQRQTEPNPEYYGVAKKKNIIKIHLESFQTFLINKKVNGKEVTPFLN
                     KLSSGKEQFTYFPNFFHQTGQGKTSDSEFTMDNSLYGLPQGSAFSLKGDNTYQSLPAI
                     LDQKQGYKSDVMHGDYKTFWNRDQVYKHFGIDKFYDATYYDMSDKNVVNLGLKDKIFF
                     KDSANYQAKMKSPFYSHLITLTNHYPFTLDEKDATIEKSNTGDATVDGYIQTARYLDE
                     ALEEYINDLKKKGLYDNSVIMIYGDHYGISENHNNAMEKLLGEKITPAKFTDLNRTGF
                     WIKIPGKSGGINNEYAGQVDVMPTILHLAGIDTKNYLMFGTDLFSKGHNQVVPFRNGD
                     FITKDYKYVNGKIYSNKNNELITTQPADFEKNKKQVEKDLEMSDNVLNGDLFRFYKNP
                     DFKKVNPSKYKYETGPKANSKK"
     gene            complement(15072..16076)
                     /locus_tag="O0293_000921"
     CDS             complement(15072..16076)
                     /locus_tag="O0293_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HCY0279815.1"
                     /translation="MSIIIEKSGLFSSFQDFGRRGYEHDGVIPCGALDTLAHEIANRL
                     VANDKNEATLEMTNKMATIRFTEPTLIALAGGNVKAYTEHMTISPYKLYLLDKGDVLK
                     FRETSYTSRVYLAVGGGFELDAWLGSNSTDFNVKIGGFKGRTLQDGDEIKLKRDYTAR
                     HHKLFENLAHTKQTDWGIDGYALSFNYMSDVFHVVKNKGTEDFKEDAIQRFVKHDYKV
                     TSKANRMGMMLEGEKIKAFYEDMPPYQTVKKGTIQIKRDGTPIILLNDHYTLGSYPQI
                     GTIASYHLTKLAQKPQGSRLKFQFIDILTAEKNLVKYSNWLNQLFHGIEYRMQLEMMK
                     "
     gene            complement(16060..16770)
                     /locus_tag="O0293_000922"
     CDS             complement(16060..16770)
                     /locus_tag="O0293_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HCY0279816.1"
                     /translation="MKIYSQGDQAIVVAIEKEVSKSLTEDLLTLRSYLIEQNYPFIIE
                     IVPSESDMMIVYDARDMIKHHNIQSPFLYMKALIESIHLNIKHDFNQQDLIEIPIVYG
                     SKYGPDLESLLKHYKIKLETFIELHSKAQYFVSMMGYSPGFPYLTGLNKKLYINHTSK
                     QKKFIPAGSVVLEGKKCGIVTTDTINDWLVIGYTPLSLFNPKESDFARLKLGDNIKFR
                     PINENELEVGAFKDVNHN"
     gene            complement(16858..17064)
                     /locus_tag="O0293_000923"
     CDS             complement(16858..17064)
                     /locus_tag="O0293_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279817.1"
                     /translation="MIIVYIVLLLILVYVNYRLVNRLLSENRIYVVRLIATITTVISF
                     ILVYALIHELMPFVVRAMDLMYHQ"
     gene            complement(17130..17738)
                     /locus_tag="O0293_000924"
     CDS             complement(17130..17738)
                     /locus_tag="O0293_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HCY0279818.1"
                     /translation="MQLFETMKIDNGHIPRLTYHTNRIKCSSERLNFKFDEHAWRNEL
                     NDVTTKYHSGQYRLKIVLNAESKFETIVSPLPEKSSFTAKFQVLPKVVNPTFIKNKTT
                     ERKHLAHNHETDLILLTSEDGKVLEFDIGNIVIEEDGKWYTPSYKDDFLKGCMRDYLI
                     DSDKLVEKDFNKNELIYKYHNNEIRLFLINSLREVADVHLCL"
     gene            complement(17738..18889)
                     /locus_tag="O0293_000925"
     CDS             complement(17738..18889)
                     /locus_tag="O0293_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HCY0279819.1"
                     /translation="MRIEYNYRYYLTENEYKQYHIQLKGFIKKYVATKLADVGEVIHF
                     AQAQQRQGRYVSLYLSYEAAKYFNHAMCTHSLAKDDIYAAAYSFEKAESINSTYEHQT
                     SYVSKYHFSFVESSEVMMTNIKRVQQAIVEGETYQVNYTARLTDNIYYPISTLYERLT
                     QFSNGNYTALLQTDEIQVASISPELFFQKGQFNNVDNVIISKPMKGTMPRGKTEAEDQ
                     QYYKTLQTSSKDRAENVMIVDLLRNDIGRISQSGSIKVYKLFFIEAYKTVFQMTSMVS
                     GTLKNNTDLTQILTSLFPCGSITGAPKLNTMKYIKQLESSPRGIYCGAIGLLLPTEDD
                     KMIFNIPIRTIEYKYGQAIYGVGAGITIDSKPKDEVNEFYAKTKILEML"
     gene            complement(18873..19466)
                     /locus_tag="O0293_000926"
     CDS             complement(18873..19466)
                     /locus_tag="O0293_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HCY0279820.1"
                     /translation="MILVIDNNDSFTYNLIDYIKTQTKLTVQVVGIDNLLIEDVINMK
                     PKAIVISPGPGNPDDYPILNEVLEQFYQRVPILGVCLGFQCIVSYFGGNIIHGYHPVH
                     GHTTQLRHTNEGIFQGLPQNFNVMRYHSLIADGATFPNCLKITAKNDEAIIMAFEHIR
                     FPVFGVQYHPESILSEYGYRQVELFLSKVGDYCENRI"
BASE COUNT         6148 a         3439 c         2769 g         7115 t
ORIGIN      
        1 acattatatt gaagtctggg gcaagttcac accacgtggt ggaatttcta ttgatcctta
       61 tacaaactat ggacgtccaa attctaaata tgaaaaaatg gctgagcatc gtttgatgaa
      121 tcatgattta tatcccgaaa aaatagataa tcgttaaatg tatcatttaa taaacacacc
      181 aataagttga ttttcctaac ttattggtgt gtttttcatt tagcatacat aataggttac
      241 attaaaataa cattttatac caaagtacac caaaagaata ttagtacacg aattaaacaa
      301 catttttata gaaacctatt gcactttaac gtcaataagt atatttttat attatctcta
      361 attaattgtg cgcgcttaat aacagaatat tctcaatatt tttatttttt tgtgatttgt
      421 tggaatattt agttgataag gcacaatcaa atttacttaa actattgtat taggggaaga
      481 aaggatggga tgtatacatg acacaacaaa actcccatgg aaatcaaatt caagacatac
      541 ctcaaacagg atttttcggg catcctcgag gactaggcgt actcttcttt gtagagttct
      601 gggaaaggtt tagttattat ggcatgcgtg ccctactcat tttctacatg tactttgccg
      661 taacagataa tggccttgga attgataaaa caacagctat gtcaattatg tcagtttatg
      721 gttcattaat ctatatgaca tcaataccag gcggatggat tgctgacaga attacaggca
      781 ctagaggcgc tactttatta ggtgcagtct ttattattat cggacatatt tgtttaagct
      841 taccatttgc attaatcggc ttattcacat caatgttctt catcattatt ggttcaggtt
      901 taatgaagcc aaacatttca aatatcgttg gccgtttata tcctgaaaat gatagacgta
      961 tggatgcagg ttttgttatt ttctatatgt cagttaatat gggtgcatta ttatcaccta
     1021 ttattttgca acactttgtt aatgttaaaa acttccacgg cggattcttg attgcagcag
     1081 ttggtatggc attaggttta gtatggtatg tacttttcaa ccgcaaaaac ttaggtagcg
     1141 ttggtatgaa acctactaac ccattgacac cagctgaaaa gaaaaagtat ggtcttatta
     1201 tcggaagtgt tgttttagca attgtattaa ttatcgttat tggtgcatta actaactcat
     1261 tatcatttaa cttagttagt aatactgttt tagtattagg tattgcatta ccaatcattt
     1321 acttcacttt aattattaga agtaaagatg tcactgatac tgaacgttct cgtgttaaag
     1381 catttattcc attatttatt cttggaatgg tgttctgggc tattcaagaa caagggtcta
     1441 atgtattaaa catatatgga attgaacatt cagatatgaa attaaactta tttggttgga
     1501 aaacaaactt tggtgaagcg atcttccaat caattaaccc attatttatt ttattactag
     1561 ctccaattat ttcactttta tggcaaaagc ttggaactaa acaacctagc ctgccagtaa
     1621 aatttgcaat tggtacgttc ttagcaggtg cgtcatacat actaattggt attgtaggtt
     1681 atgcatcagg ttcatcaaac ttttcagtta actgggttat tttatcgtac attatttgtg
     1741 ttatcggtga gctatgctta tcaccaacgg gtaatagtgc tgctgttaag ttagcaccta
     1801 aagcatttaa tgcccaaatg atgagtattt ggtacttaac taacgcttct gcacaagcaa
     1861 tcaatggtac tttagttaag ttaatagaac cactaggtca aacaaattac tttattttcc
     1921 taggtgttgt tgcaattatt gttacaacaa ttgtattagc aatctcacct ttaatcatca
     1981 aagcgatgaa aggtatacgt taatattgtt ggcctaattc aaaaaacagt aagtcattta
     2041 aatggcttac tgttttttta taggtttcta ttaattaaat tcaagatatc agtataaata
     2101 aaaacttaat atgctcatta tagacagcta taactatatt ttctcgtccc actctataga
     2161 aatcaatttc atttattacc attttctcta tttaaaaaca tatttttggt attaacaatt
     2221 aaaagtgggt atatatattt aatgaaccaa tttttagaaa gtaggctaaa ctatggaaaa
     2281 taaatataca catggtgtcc tcttttacca tgaacacagt ggattaaaaa atattaatca
     2341 aggtattggg gaagttacaa cagcattaag ttcaatttgt aagcatcttt ctattcaatt
     2401 gagtgaaaac gaaggtgaca ttattaaata ttgccaagaa atcaaaacta aaaactatgc
     2461 aaaagatgta gatatattat ttattcttgg cggtgatggc acggttaacg aacttataaa
     2521 cggtgttatg acacatgacc ttcaactccc tattggcatt ttaccaggcg gtacttttaa
     2581 tgattttaca aaaacgctaa atatagcacc taatcataaa caagctagtg aacaaatgat
     2641 ttctgcacaa gttggtacat acgatgtaat taaaataaat aatcaatatg cactcaactt
     2701 tgttggctta ggtctcattg ttcaaaacgc tgaaaacgta caagatggtt caaaagatat
     2761 atttggtaaa ttgagttata ttggatcaac tgtcaaaaca ctgctaaatc caacacaatt
     2821 taattatcaa ttatcaatag atgataagac atattctggc gaaacgacca tgatactaac
     2881 agcaaatggt ccattcattg gtggtagtcg tataccattg actgatttgt ctcctcaaga
     2941 tggtgaactg aacactttca tttttaatga acaaagtttc agtattttaa atgatatttt
     3001 taaaaagcgt gatagcatga attggaatga aattactcaa ggtattgagc atatacctgg
     3061 taaaaagatt tcacttacaa cagatccagc aatgaaagtt gatattgatg gcgaaatttc
     3121 attagaaaca cctattgata ttgaggttat accaaatgcc attcagttac ttactgtcaa
     3181 tgatttgtaa gcatccggaa tgatcttaac ccgatattat attaggtttg gagtgtaatc
     3241 ataagattca acgcacctta tattcgtttt tattagcttt aagctattac cctgtttcaa
     3301 aaacagacat ataattttgg cgcatgccac taaaatgatt gatgttgtat tttaaatcat
     3361 agtggtttat gcgtcttttt caaattctat aaaaaatcgg atgacgtgta atctgccata
     3421 gattaacaca ttcatccgat ttataataat aagataaact aacatttatt gagagcggga
     3481 cggaaatgat aaagaacgac taatgattga ttatgtagcg attctttatc attagtcaca
     3541 gctaatgtgt acttaaaaat atgaatgcat gagttacact catgcataag aaatactaat
     3601 ttctaaagaa aaagtatttc tttatgttgg ggcccacccc aacttgcatt gtctgtagaa
     3661 attggcgttc caatttctct atgttggggc cccgcagtca actactgcca atatagtgtt
     3721 gtagtgtcta agacataaaa gttcatctca gtcacaatta ctttatagcc tatccatcat
     3781 cttttctaca tgaaattttt caagtgatat attttatttc tcaatattat ctaaaaaata
     3841 ctgttctaca tctttccagc tatttacgcg ttcaaatcga tcatcattaa tattatgcac
     3901 tgctgtaaac ataatcggtg taccagtaaa aatttcaagc tgtctaggat tgtcatctat
     3961 taaataatca gctttaacga tgttttttct accacaaaaa acaaaatgct gaggatctaa
     4021 aaatggaaag aactctagta accattcata tttatcacta aatgatgttg gtacatccat
     4081 tgctgctgta gcaatatata catcataatg ttcagttaat tttttcacaa cttcttgtgc
     4141 atacggcatc actttaagat gtctgaagaa gcctgattct ctcaatactt ctgtaattaa
     4201 tccatcatgt tcaggaataa catgtttaag tttttgacca ttcaaagctt ccattttaat
     4261 acctaaatcc gctctaaaat tgacagcatc aatgatttct cctaatgtat ctgccaatac
     4321 ttcatccata tcaatcgcga ttgatttacg ggtcatttta atcttctcct caaaatatca
     4381 gtattgattc agtatagcaa aaaagcaatt gatacaaaac aatatcatct attgttgctt
     4441 tttacactga atactctcac ctcatcattc acactgcatt gagcttgaga cataaatcaa
     4501 tgttcaatgc tctacaaagt tatattggca gtagttgact gaacgaaaat gcgcttgtaa
     4561 caagcttttt tcaattctag tcaaccttgc cggcggggcc ccaacaaaga gaaattggat
     4621 tcccaatttc tacagacaat gcaagttggg gtgggacgac gaaataaatt ttgcgaaagt
     4681 atcatttctg tcccactccc cacttactat tcgtatttaa agtttgataa aacttctaac
     4741 attttatcat tttgttcttt aaaaccaatt gtaattctaa caccagttgg aaatggtcgc
     4801 gtaatacacc ctacatttaa aagtgcttca taaagttcat ttacccgctt tgtttttaca
     4861 aatataaaat tcgtttgact tggcaagaaa tactcacttt gaggtaattg ataaaatctt
     4921 tcgcgttcaa cactattttt atgtgttacc tcttttaaat attgttgatc ttcaagtgct
     4981 gcaactgctg cgtattcaga tatacgtgtc acattaaatg gtggtctaat gatgttccat
     5041 ttttcaatcg catgttcact tgctaccaca tatcctacac gtaaacccgc taatccatac
     5101 gcctttgaaa atgtacgtaa taagaaagca ttgtcatatt tttgttgcaa agcaagtgta
     5161 tccgggtagt cctctgctgt cacaaattca aaataagctt catctataat tactggtaca
     5221 tgtggaggta cttgagataa aaacgaatct aagctctcat gattaaaata tgtacctgta
     5281 ggattatttg gattacataa ccataccaat gacgtatctt cattaacttc ttttaaaata
     5341 ccttctaaat cgaagccacc atcttttaaa ggtacttgta tcacattagc tgattcaaca
     5401 atcgcattgt gataatattg accgaatgtc gcttcacttg taacaatagt atcccctggc
     5461 gttaatacag ctctagaaat cattaatata acttcatcta atcccgcacc aaataaaatg
     5521 cgtgattgat ctacatttaa atgtttacta atcgccgctt ttaatgtcgg tgatcctgtt
     5581 tcaggataat aatataactc atctaagtgt gctgatatcg cttctttaac tttaggcgat
     5641 ggtccataca aattttcatt tgatgcaagt ttatataaat ctccttcaat gccatacttt
     5701 tctttcaatg cccttggaga taaaccaggc tgatatgctg atagttgatt aagttgttct
     5761 ttcataataa aaacaacctc cttagaatta actgaaattt caaaaaactt atattcatta
     5821 tatcgctcta tgtttctaaa atgcaatgct actattttca catcataata gcttacattg
     5881 tgtttaacat gtcatatttt ttaatcatta ttgcatgttt aaattgaaca taattataga
     5941 tttaaatata acaacgccac ataagatata atttggtgtt acgcatctta tgtggcgttg
     6001 gtcgttattt gattaaccct tttgctttta aataatcttt cgcaactgta tatgggtctt
     6061 catttttaac tgttacttta tagttcatca tttgcatatc ttcatcagat attttgtttt
     6121 ctagtttgtt taacggtttc ttaatttctg gatgtttctt taaaaagctt tctttaaata
     6181 atggtgctcc ttgatatggt ggaaatacgt gcttatcatc ttttaacaca accatatcat
     6241 attgttttaa ttcagcgtca gttgaatatg catctattaa attaatatta cctttattga
     6301 tcgcttgata tctcaactta ggttccattg tacgtatgtt atctaaattt aaattataag
     6361 ccttttgaac agctttgtaa ccatctggac gatcattaaa ttccaatgta aatcctggtt
     6421 taagttgatc tttaacctta tttaaatcac caattgtacg tatattatgt tgtttagcaa
     6481 aatcacgttt tacagctaaa gcatacgtat tgttatactt cattggtttt aacatagtca
     6541 tttgatattt cttttcaaga ctttgcttag cttgttcata aacttttttc tcttcttttg
     6601 acttcaatgg ttcttttgtt aattcaccta aaactgttcc agtaaattct aaatacccat
     6661 ctatatcgtc agattttaaa gcattaaata aaaatgctgt tttgcccata ccatctttca
     6721 cttctacagt atttttggtc tcttcttcta ttaaaatttt atacatattt gtaataatcg
     6781 atggctcgga gccaagcttt ccagctaacg taattttatc acctttttgt gcaaacatag
     6841 gaatagcgat agccagtata ataatcatca ctataacacc taacgtcatc aataactttt
     6901 tataagataa tttagccata aatcttaaaa ttaaatcaaa tataattgcc aataaggctg
     6961 ctggaattgc acctaataat atcaacgatg cattgttacg gtctatacct aataaaatta
     7021 aatctcctag tccgcctgca ccaattaatg ctgctagtgt tgctgtacct ataattaata
     7081 ccatagccgt tcttacacca gccattataa caggcattgc tatcggaagt tcgactttag
     7141 ttaaacgtct aaatggtttc atacctatac ctttagccgc ttcaatgagt gatggatcaa
     7201 cttctttaat tccagtatac gtattcctta aaattggtaa caacgcatac actacaagtg
     7261 caataattgc tggcacacga ccgataccaa ataaaggaat cattaaacct aataatgcca
     7321 acgatggtat ggtttgaaga attgccgcaa tattcattac gatttcagat atcgttttag
     7381 tcttcgttaa taaaatacct aatggtaccg caatagcagt tgcaatcaat aatgcgataa
     7441 atgatatttg aatatgttct atcattgtcg aaaagagttg ccccttacgt tcactcaata
     7501 tgtcgaaaaa gttagtcatg ttgagctacc tcctttttct gggacaaata tttgaagata
     7561 tctttcctat caataacata ttgacctacg ctatcttctt gcatgacaat gacacgctcg
     7621 ctctctgata aaagttgata caatacttca attggttgat tgtcataaac aattggataa
     7681 gcgctcatag atgtaacctc atcgattggt ttcataatat ccaagtcacg gataattgcg
     7741 ttctcttcaa cacatggcgc atcatcttct aaatgactac ccataaattg tttaacaaat
     7801 tcactttgag gattattttt aaatccttct ggtgtgtcaa tttgttcaat atgcccttca
     7861 ttcaaaagac aaatcttatc accaagtttc atcgcctctt gaatatcatg tgtaacaaat
     7921 atgattgtct tcttaatttt agtttgtaat tcaattaaat catcttgaag tttttctcgg
     7981 ctgattgggt ctaatgcact aaacggttca tccattaaaa taactggtgg atcagctgct
     8041 aacgcacgta taactcctac acgttgtcgt tgcccccctg acaattcatc aggttttctg
     8101 tttttatatt tttcaggttc taatccaacc atttcaagta attcatctac tcttttatct
     8161 atatcttttt ctttccactt tttcatttgt ggcacttgtg caatattttc tttgattgtc
     8221 atatgtggga ataatgcaat ctgctgcaat acgtatccaa tatcccaacg catttcgtat
     8281 actggataat cacttattgg tttatcttta aaataaatat aaccttcact taagtgaatg
     8341 agtcgattaa tcatttttaa tgtcgtagtt tttccacaac ctgaaggtcc aattagcaca
     8401 aaaaattcac cctcattaat attgaaacta atgttatcga cagcaacatg tttgccataa
     8461 cgcttagtta catttttaaa cttaatcact ttgccacctc tttttttctc atagcataaa
     8521 accgagatta tatgtatgta ttccctattt aaccacgttt attacaattt tcaaatttaa
     8581 atgatttatc cttgaacttt tttaataaaa taatgaataa taggtaatct ccagttaaga
     8641 aatagtgtta ttttaccttg aattcaaaaa aacacccagt aaaacaagga atgcttacta
     8701 ggtgtcttca ctatactttg gctttataat tttgaatcgt ttctaaaaat gctggacaat
     8761 aatgttttaa tttgtaacta cctacgccat caatattaat catatcttgt ttcgaagcag
     8821 gcttacgttt agcaaattcc tccaacgtgt aatcagaaaa tatacttaca ggtgctatcg
     8881 ttaatttgtc acttaacttt ttacgaactt ctaccaactg actgaataat actcggtcaa
     8941 ccccttcaac cgtatttata aatacttttt cagtcgcttt ttgcttaaat ggtgttgtga
     9001 atacttctac ttcattactg agtaattttt taattgaagt atcacacatt aatatttcgt
     9061 cattttcatt taagaaccct ttgaatctta attcatctat taagtgactt aattctgatg
     9121 ttgtgtaacc tttcattaaa ccatgggttg aaatttggtc ataaccttta tacttaatat
     9181 aatctgttga ctctcctctt aacacttgaa tgataacact ataactctct tgttgtttca
     9241 tacgagcgat gcaactaata atcatcttag cttcttgtgt catattatat gatttatctt
     9301 gttgaacaca attactacat tgttcacatt cttctaattt ttcattcggt tcaaaataat
     9361 ggacaattgt tgcttctaga catttttttg tttttgtata ttgaatcatt ttagttaact
     9421 tttcgcccat tttatcttta tagtcatcat cagcttgaga gactgttata aaatactcgt
     9481 gtaaattgat atcgcgttcg ctaaataaca aaatacattc acttttcaac ccgtcacgac
     9541 ctgcacgacc cgcttcttga taataagatt ctaaatctcc aggcatatta taatgaataa
     9601 caaagcgtac attggattta tcaataccca taccaaaagc atttgtagca acgactactt
     9661 taacacgatc aaataagaaa tcattctgcg cttcttctct ttctttattg ctcaaacctg
     9721 catgatatat aacactttca attttctgac tttctaaggc ttcttgaagc tcttcaactt
     9781 gcttacgtgt agaacaataa ataatacctg catcttcatc gtgtgtttta atataatcca
     9841 atataaattt ttgacgttga taagtaggat ttactttaaa aattaagttt ctacgcttag
     9901 tactcgtttt aatttgatca gtttgagcga tatttaactt ttctctaata tcttgctgta
     9961 cttcaaccgt ggcagttgct gtcaacgcta ttattgtaaa atcttgaggt aacgtaaata
    10021 cttttgaaat aacattttgg taactcggcc tgaaatcatg accccattta gaaatacaat
    10081 gcgcttcatc aaacgcgact aagtgaatct ttatacgctg aagcatattt aaaaaatatc
    10141 ggttttcaaa tcgttctggt gcaacataca aaaattgaat ttctccattt gataatgctt
    10201 tttcaatacg ttgttgctct ttttgagtca aactactatt taaaaaagca gcttgaattc
    10261 ccatcgcttt taattgatcc acttgatctt tcattaatga tattagtgga cttattacaa
    10321 ttgttgtacc acctaacaat aaacctggta cttgatagca tatagactta cctccaccag
    10381 ttggtaagac accaagcaca ttacgatggt ctaatacttt gctaataatt tcttcttgtc
    10441 ctggtcgaaa cgtttcatac ccaaagtaat gcgataatgt ttgttgcatc ataaattgac
    10501 cccttattgt tgttctttaa tttcttctaa ctcactccat cttgtgatgt ctaaatcata
    10561 ttgaatttca agttgttctt tttcttcgtt taattcttta attttcccat aatctgcact
    10621 tgcctcaatc atgagcacat caatttcttc cattcttact tccgcttgtt ctatgcgttt
    10681 catcaattgt tcatattcta atttttcttt atatgataaa ccatttttct tacgtacagt
    10741 tgtagaagat ttagattgtt gcttcaatgt ggatttattt ttatctaatg attttttata
    10801 actttcataa tcttcaaaag ttccgataat cttttccatc tgaccatcat gaataaacca
    10861 atatgactgt gcaactttat ttaagaagta gcgatcatgg cttacggtaa tcactgtacc
    10921 accgaaagta tgaatataat cttcaagtat tgttaaagtc tcagtatcta aatcatttgt
    10981 cggttcatct aacaacagaa catttggctg gtgtacgagt agacgtaata aatacaaacg
    11041 cttttgctct ccaccagata atttataaac ttttttacca tgagttgcac ttggaaataa
    11101 aaatcgttca agaagttgtg taatagatac cgaggttcca tctttttctt ttgcgatctc
    11161 attttcttct cttaaataat caatcatacg aatatctcta tccagggtct catctgtttg
    11221 cttaaaataa gctactttaa ccgtctgccc agtcttcaat ttaccttcga attgttggtc
    11281 ttctccactc aaaatattta agagtgttgt tttaccagct ccatttggcc caacaacacc
    11341 aatacgctca cctttttgaa taatttccgt cagatgttca attaatactt tatcattaat
    11401 agcctttgat aagtcttcta attcgaacac ttgcttacct aatcttgaat aagcaagatt
    11461 caattcacct ttatcgtctt tatattgctg gttaacttca ttttctaggt cattaaatcg
    11521 attaattcta gcttgttgct ttgtagtacg agccttagct ccagccctca tccaagcaag
    11581 ttcttcctta tataaagctc gttgcttttg ttgttgcttt tgaagtgtta cttctctttc
    11641 agcgcgcatt tcaatataag attcatagtt accaggatat gacgctaact tacctctgtt
    11701 tagttcaata attctagtgg aaacttcatt taaaaaatat cgatcatggg ttacgaataa
    11761 aacagtatga ggatattgct tcacataatt gatcaaccag ctgattgatt cgaagtctaa
    11821 atggttcgta ggttcatcta ataacaataa atctggttgt tctattaatg ttttagcaag
    11881 tacaacacgt ttttgttgtc cgccggataa ttctttaatg tatttagtag tatcatgtat
    11941 acctagtttt gagaggattg ttttaatttc agcgttatag tcccaagcat catgttgatc
    12001 catagcatct tgcgcatcca tcattcgctt gaacaattta tcactttggt catccgcata
    12061 agcttgtact gcctgctcat attgcttgat gatgcgtaaa gttgttgtat cagaacttaa
    12121 tactgcatca aaaactgtca tatcttcatt aaggtcctgt ttctgagacg aatatcgaat
    12181 tcgatattga tttggatgca taacattggc tgtaaaatca tcatcaatac caccaattac
    12241 ttttaacaac gtacttttcc ctgtaccatt tatgcctact aaacctattt tttcaccttc
    12301 tgaaattgat aaatctaggt tatcgaatat agtcttatcg gcataagatt tatttaaatg
    12361 ttcaatttta tatgcttcca tacccatctt ccttcttctg ttgaaatctc tacgtactta
    12421 aatcagttct atacattata cacctttttg aaatcaaaag aaatcgatac cgttaagtgt
    12481 cataatttaa tatattgttg cttcaactat aaataaaact tgttaaggct ttgatgacta
    12541 tgctaatgat ctttataata taccttttta tcaacacata taatgtaaaa cataatataa
    12601 aagaacataa taaaaacccg gattgatatt ttatcaatcc gagttcgtgt ttagatatta
    12661 ttttttagag tttgctttag gtcctgtttc atatttatac ttcgaaggat ttaccttttt
    12721 gaagtctgga tttttgtaga atctaaacaa atcaccatta agcacgttgt cactcatttc
    12781 gagatccttt tcaacttgct ttttattctt ttcgaaatca gctggttgag tagttatgag
    12841 ttcattattt ttattagaat aaatcttacc attaacatat ttataatctt ttgttataaa
    12901 gtcaccattt ctgaatggaa ctacttgatt atgaccttta gagaataaat cagtaccgaa
    12961 cattaaatag ttcttcgtat ctataccagc caaatgtaaa attgttggca ttacatcgac
    13021 ttgaccagca tattcattat tgataccacc agatttacca gggattttaa tccagaaacc
    13081 agttctgttt aaatctgtaa atttagccgg tgtgattttt tcacctaata gtttttccat
    13141 ggcattgtta tggttttcag agataccata gtggtcacca taaatcataa tcactgaatt
    13201 gtcatataat ccttttttct tcaagtcatt aatatattct tctaatgctt cgtctaaata
    13261 acgtgctgtt tgaatataac catcaactgt tgcatcacct gtgtttgatt tctcaatagt
    13321 tgcatccttt tcatctaatg tgaatggata gtggttagtc aatgtaatta aatgagaata
    13381 gaatggtgat ttcatcttag cttgataatt agcagaatct ttaaagaaaa ttttgtcttt
    13441 caagcctaag tttacaacgt ttttatctga catgtcatag tatgttgcat catagaattt
    13501 atcgatacca aagtgtttat atacttggtc tctgttccag aatgttttat agtcaccgtg
    13561 catgacatca gatttgtagc cttgcttttg atctaaaatt gctggtaatg actgatacgt
    13621 attatctcct tttaatgaaa aggcagaacc ttgcggtaaa ccgtataaac tgttatccat
    13681 tgtaaattca gagtcagatg ttttaccttg acctgtttga tggaaaaagt tagggaagta
    13741 tgtgaattgc tctttcccac ttgataattt gtttaaaaac ggtgttactt ctttaccatt
    13801 aaccttttta ttaattaaga aggtttggaa actttctaaa tgaatcttaa taatattttt
    13861 cttctttgcc accccataat attctgggtt aggctctgtt tgacgttgtt tcgtataatt
    13921 taatactttt gttaagtcat cttcagatgc tagcgctttt tgttgattat tttcgatagt
    13981 tttaacacca tcgtatactg taaagttata aggtcctaaa tatttcacta aatatttatg
    14041 gtcaaatgta cgtgttaata attctggtct gtcagtttca gcaaaagcta agtttaagaa
    14101 gaataatgct actgaagccg ccattacgac aggaacaaat ttcttactaa atgcttttgt
    14161 gtctaaccat tttgttttaa atattaaaat gaataagtaa actaacgtat caatgaaata
    14221 aacaaagtca taccatttga atgatgcact aaccgcacca cccatagatt ctacgttacc
    14281 tacttggttt aaagtactaa acgttaaaaa atcagagaag aatctaaagt acacaacatt
    14341 ggcatataat aggaacgtca ataagaagcc gcctatgaac atgaaccaaa atgctttttt
    14401 gcctttaaag aataggaaca cacttaaaac cagtgctact aaactataag gattcatcaa
    14461 taatattaag ttttgtacta aacctttaac acctaaagaa aaatcaacat aataagaaaa
    14521 atacgtcttc aaggtaatcg ttattacggt taataagaag aacgcaaaaa gactaatttt
    14581 ctttttttgt gaactcatga ttctttcccc cgttatttag ataataaatc ttgcgaaagt
    14641 acttgaataa attagatatt aacattatgt agttaattaa taagtgcgtt taaaagttaa
    14701 cattgtcata attatagtaa ttactttcag attcaagttt ataattttcg ttatttgtag
    14761 ctttcaactt tgatttaaca agtattttgg gctacaactc atatttgctt gtgtaagttt
    14821 ctttatttaa tattatcatt caattaacaa atttaaaatt tgtaatcaaa ttgtaataat
    14881 aagcatagct caattaataa atatagagta attttagtca aaattcaagt aaaaacacac
    14941 tttatctttt cattttactg tgaaacgaat tagaaaatga tacatttcgc agataaaaca
    15001 cagtattatt tcattataca ttaggaggaa attatttgca tcggactcga gtatgagcta
    15061 cgtaccaaat attatttcat catttctaat tgcattctat attctattcc atggaataat
    15121 tggtttaacc agttactata cttaacaagg ttcttttcag ccgttaaaat atctataaat
    15181 tgaaatttca aacgtgatcc ttgcggtttt tgtgctaatt tcgttaaatg ataacttgcg
    15241 attgtaccga tttgcgggta gctacctagc gtataatgat catttaatag gataataggt
    15301 gtgccatcac gcttaatttg tatcgttcct tttttgacag tctgatacgg tggcatatct
    15361 tcataaaaag ctttgatttt ttcaccttca agcatcatcc ccatgcgatt tgctttgctc
    15421 gttactttat aatcatgttt cacaaatctt tgaatggcat cttctttaaa atcttccgta
    15481 cctttatttt taacgacatg aaatacatca gacatataat taaatgacaa ggcgtatcca
    15541 tcaatacccc aatctgtttg tttcgtgtga gcaaggtttt caaataactt atgatgacga
    15601 gctgtataat ctctcttaag ctttatttca tcgccatctt gtaatgttct acctttaaaa
    15661 ccaccaattt ttacattaaa gtcggttgag ttagatccta accatgcatc taattcaaat
    15721 ccgcctccca cagctaaata cactcgcgat gtataacttg tttctctaaa ctttaaaaca
    15781 tcgcctttat ctaacaaata caatttatat ggagatatag tcatatgctc agtgtaagct
    15841 ttgacattac cccctgctaa tgcaatcagc gtaggttctg taaaacgaat cgttgccatt
    15901 ttattagtca tttccaaagt tgcttcattc ttgtcatttg caactaatcg attagcaatt
    15961 tcatgtgcta aagtatcaag tgcaccacat ggaattacac catcatgttc atatcccctt
    16021 ctgccaaagt cctgaaagct actgaataag ccactttttt caattatgat tgacatcttt
    16081 aaacgctcct acttctaatt cattttcatt gataggtcta aatttaatat tatcgcctaa
    16141 ctttaagcgt gcgaaatctg attctttcgg attaaaaagt gataatggtg tataaccaat
    16201 aactaaccaa tcattaattg tatccgtagt tacaataccg cattttttcc cttcaagtac
    16261 tacagaacca gctggaatga attttttctg tttactcgtg tgattaatat acaatttctt
    16321 atttaatcca gttaaataag gaaacccagg tgaatatccc atcatcgaaa caaaatattg
    16381 cgccttagaa tgtaattcaa taaaagtttc tagcttgatt ttgtaatgtt ttaaaagtga
    16441 ttctaaatcc ggaccatatt tcgaaccata cacaattggt atttcaatca aatcttgctg
    16501 gttaaaatca tgttttatgt ttaaatgaat cgattctatt agtgctttca tgtataaaaa
    16561 aggtgattgt atattatggt gtttaatcat atcccttgcg tcatagacaa tcatcatgtc
    16621 tgattctgat ggcacaattt ctataataaa tggataattt tgttcaatta aatatgagcg
    16681 aagtgttaat aaatcttcgg ttaaactttt agatacttct ttttcaattg cgactacaat
    16741 ggcttggtca ccttgactat atattttcat ttgtacacct cttcaatatt tggttacttt
    16801 actttcattt ttaattatag aatcgattga atagttcatt ttacctactc actaaattta
    16861 ctggtggtac attaaatcca ttgcccgcac aacaaaaggc ataagttcgt gaattaatgc
    16921 gtatacaagg ataaagctta taacagtagt aattgttgct atcaaacgaa caacatatat
    16981 tctattttca gatagcaatc gattcactaa tcgataattt acgtatacaa gaattaacaa
    17041 cagcacaata taaacaataa tcatatttgg ccccatttaa tttttaaatt tgttttcaca
    17101 tcattttcta ctttattatc atactaattt taaaggcaaa ggtggacatc ggcaacctct
    17161 cgtaaactat ttatcaaaaa taaacgtatc tcattgttat gatatttata aatcaattcg
    17221 tttttattaa agtctttttc aacaagtttg tcactatcta ttaaataatc acgcatgcat
    17281 ccttttaaga aatcatcttt ataacttggt gtgtaccatt ttccatcctc ttcaatgaca
    17341 atgttgccaa tatcaaattc aaggaccttg ccgtcctctg aagttagcaa tattaaatct
    17401 gtttcatgat tgtgtgctaa atgctttcgt tctgtcgttt tattttttat aaaagttgga
    17461 ttaactactt tgggcaacac ttgaaatttt gctgtaaaac tacttttctc aggtaaaggt
    17521 gacactatcg tttcaaattt gctttcagca tttaatacga ttttaagtct atattgacca
    17581 ctgtgatact ttgttgttac atcgtttaat tcatttcgcc atgcatgttc atcaaattta
    17641 aagtttaatc gctcagaaga acattttatg cgattagtat gataagtaag tctagggata
    17701 tgtccattat caattttcat tgtttcaaat aattgcatta taacatctcc aaaatcttgg
    17761 tttttgcgta aaattcattc acttcatctt ttggcttaga atcaattgta atacctgctc
    17821 cgactccata aatcgcttgt ccatatttat actcaatagt gcgaatcgga atattaaaaa
    17881 tcattttatc atcttcagtt ggaagtaata gtccaattgc tccgcagtat ataccacgag
    17941 gtgaactttc taattgttta atatatttca ttgtattcag tttcggtgca cctgtaatcg
    18001 aaccacaagg aaataacgat gttaaaattt gagttaagtc tgtattattt tttaaagttc
    18061 cacttaccat cgaagtcatt tgaaatacag ttttatatgc ctcaataaaa aatagtttat
    18121 acaccttaat tgagccactc tgtgatattc tccctatatc gtttcttagt aaatcaacaa
    18181 tcatgacatt ttctgcacga tctttcgaag aagtttgcaa tgttttataa tactgttgat
    18241 cttcagcttc cgttttacct ctaggcattg tccctttcat cggtttgctt atgataacgt
    18301 tatcgacatt gttaaattgt cctttttgaa aaaataattc tggtgagata gacgctactt
    18361 ggatttcatc agtttgtaat aacgcagtat aattaccatt actaaattga gttaatcgtt
    18421 catataaagt actaatagga taataaatgt tatccgttaa gcgcgccgta tagttcactt
    18481 gatacgtttc gccttcaaca attgcttgtt ggacacgttt aatattagtc atcataacct
    18541 cagaagattc aacaaatgaa aaatgatact ttgatacata agaagtttga tgttcatatg
    18601 ttgaatttat gctttccgct ttttcaaaac tataagctgc tgcataaata tcatctttag
    18661 ctaatgaatg tgtacacata gcatgattaa aatactttgc cgcttcgtaa cttaaatata
    18721 acgagacata tctaccttgt cgttgctgcg cttgtgcaaa gtgtatcact tctcccacat
    18781 cagccaactt agtagcaaca tacttcttta taaatccctt taattgaata tggtattgct
    18841 tatattcatt ttcagttaaa tagtagcgat aattatattc tattctcaca gtaatcacct
    18901 accttcgata aaaataattc aacttgtcga taaccgtatt cactcaaaat agattcagga
    18961 tgatattgca caccaaaaac cggaaatcta atatgctcaa atgccataat aatcgcttca
    19021 tcgttttttg ctgtaatctt taagcaattt ggaaaagtcg ctccgtcagc aattaatgaa
    19081 tgataacgca ttacattgaa attttgaggc agtccttgaa aaataccttc attggtatgg
    19141 cgtaactgtg tagtatgtcc gtgtacagga tgatagccgt gaatgatatt tccaccaaaa
    19201 taagacacga tacattgaaa tcctaaacat acacctagta taggtacacg ctgataaaat
    19261 tgttctaaca cttcattcaa gataggataa tcatccggat tacccggccc aggcgaaata
    19321 acaattgctt ttggcttcat attaatgacg tcttctatca gcagattatc aataccaaca
    19381 acttgaactg ttagtttcgt ttgagtctta atatagtcta ttaaattata tgtaaatgaa
    19441 tcattattat ctatgactag aatcattgta t
//
LOCUS       DAKTIO010000035        19284 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.035, whole genome shotgun sequence.
ACCESSION   DAKTIO010000035 DAKTIO010000000
VERSION     DAKTIO010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19284
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.035"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(261..1127)
                     /locus_tag="O0293_000927"
     CDS             complement(261..1127)
                     /locus_tag="O0293_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCY0279821.1"
                     /translation="MNEQWLEHLPLKDIKEISPVSGGDVNEAYRVETDTDTFFLLVQR
                     GRKESFYAAEIAGLNEFERAGITAPRVIASGEVNGDAYLVMTYLEEGASGSQRQLGQL
                     VAQLHSQQQEEGKFGFSLPYEGGDISFDNHWQDDWCTIFVDKRLDHLKDELLNRGLWD
                     ANDIKVYDKVRRQIVAELEKHQSKPSLLHGDLWGGNYMFLQDGRPALFDPAPLYGDRE
                     FDIGITTVFGGFTSEFYDAYNKHYPLAKGASYRLEFYRLYLLMVHLLKFGEMYRDSVA
                     HSMDKILQDTTS"
     gene            1345..2409
                     /locus_tag="O0293_000928"
     CDS             1345..2409
                     /locus_tag="O0293_000928"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCY0279822.1"
                     /translation="MYKLIKPFLFKIEPEKAHGLTIDALKTLQKFPVLFPVVDKLFTY
                     KNPTLSQTIQGNTYDNPIGLAAGFDKSCEVPKALEHLGFGALELGGITPKPQPGNPQP
                     RMFRLLEDDALINRMGFNNIGMNKALSHLRKNAYQVPVGINVGVNKMTPYEARYQDYI
                     KVIDTFKHDVSFFTVNISSPNTENLQNFHDKDEFSMLCQALTAFKKQHDVTVPIYLKL
                     TSDMDFDGLKALLPAITETFDGIILANTTRQRDGLTSANKVEEGGLSGRPLFERNLKL
                     IKYAYQQTNGEFLIIGTGGVFSTEDAIKMMRHGASLIQIYSSLVIEGPGLTKKMNKGI
                     ARYLKDHHFDNVSDIIGLDA"
     gene            2564..2839
                     /locus_tag="O0293_000929"
     CDS             2564..2839
                     /locus_tag="O0293_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279823.1"
                     /translation="MTKETDNKKTVATSDNKDTQQQNQPEEDERQGLNGYRKTDLDLE
                     IEQELREMMKTGENETKNDYKKFKVFSLISTLVIVILAIIRFVHKMM"
     gene            complement(3025..3747)
                     /locus_tag="O0293_000930"
     CDS             complement(3025..3747)
                     /locus_tag="O0293_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCY0279824.1"
                     /translation="MINAEPIISKMKNQKINYDKVLKKLIGQWEREAIRPKILLHSCC
                     APCSTYTLEFLTQYADIAIYFANSNIHPKNEYLRRAKVQEQFVEDFNRKTGANVKYIE
                     APYEPHKFVKMVKDKELADEKEGGLRCTACFEMRLDIVAKAAVEHGYDYFGSAITLSP
                     KKNAQLINELGMDVQKIYDVNYLPSDFKKSKGYERSIEMCNDYNIFRQCYCGCVFAAM
                     QQGIDFKTVNKEAKAFLEQYPD"
     gene            4101..4550
                     /locus_tag="O0293_000931"
     CDS             4101..4550
                     /locus_tag="O0293_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HCY0279825.1"
                     /translation="MTALFPYIAFENSKEALAYYEEVFGATDVKRLEVGEEQASHFGM
                     TKEEAQEATMHAEFEVLGVKVLCSDSFGRADKINNGISLLIDYDVNNKEDADKVEAFY
                     EQIKDHSSIEIELPFADQFWGGKMGVFTDKYGVRWMLHGQDYTAIQQ"
     gene            complement(4631..5218)
                     /locus_tag="O0293_000932"
     CDS             complement(4631..5218)
                     /locus_tag="O0293_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCY0279826.1"
                     /translation="MTIDRRIRKSQVAMRNAFIELLHQHQLEEITVQQIADLADVNRS
                     TFYTHYYDKYDLLEKLEDQQLEEIRDFIHDEKLNGAVKLSTDNIHQIMTVLIEMIEKD
                     IAFYQLMFRMGKDSNIHEKLYDLIMCHLQRYKNENDQINGIPFSYFMSYVSGAGLSFI
                     RHWVEDPNRIDKVDLIRHFNTIVNQGPATIIKNIQ"
     gene            complement(5196..5294)
                     /locus_tag="O0293_000933"
     CDS             complement(5196..5294)
                     /locus_tag="O0293_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279827.1"
                     /translation="MPIINVGDLWDNEHNVYNHWGGLDINDYRSTY"
     gene            5351..7033
                     /locus_tag="O0293_000934"
     CDS             5351..7033
                     /locus_tag="O0293_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HCY0279828.1"
                     /translation="MNQHLLGNPKLTVTHVNEVKAGINHIVVDSVQYGNQEMIMEKDV
                     TVEMRDGEKLYINIFRPNKDGKFPVVMSADTYGKDNKPKITNMGALWPTLGTIPTSSF
                     TPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAANQSWSN
                     GNIGTNGVSYLAVTQWWVASLNPPHLKAMIPWEGLNDMYREVAFHGGIPDTGFYRFWT
                     QGIFARWTDNPNIEDLIQAQQEHPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNR
                     GSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVI
                     YEVRDQFYKGEFKSASAFPLPNAEYTPLYLNAENHTLNHAKISSAHVAQYDSEDKQQD
                     VSFKYTFDKDTELVGNMNLKLWVSTKDSDDMDLFAGIKKLDRRGNEVNFPDFNHIENG
                     QVATGWLRVSHRELDQEKSSIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGET
                     LEVVVKGSEIVIGNSTPGMKTRYEHEETVNKGMHMIYTGGKYDSQLIIPIVN"
     gene            complement(7162..7545)
                     /locus_tag="O0293_000935"
     CDS             complement(7162..7545)
                     /locus_tag="O0293_000935"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015901315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCY0279829.1"
                     /translation="MIRTMMNAKIHRARVTESNLNYVGSITIDSDILEAVDILPNEKV
                     AIVNNNNGARFETYVIAGERGSGKICLNGAASRLVEVGDVVIIMTYAQLNEEEIKNHA
                     PKVAVMNEDNVIIEMIHEKENTIVL"
     gene            complement(7547..8398)
                     /locus_tag="O0293_000936"
     CDS             complement(7547..8398)
                     /locus_tag="O0293_000936"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCY0279830.1"
                     /translation="MTKLITTVKEMQHIVKAAKRSGTTIGFIPTMGALHDGHLTMVRE
                     SVSTNDITIVSVFVNPLQFGPNEDFDAYPRQIDKDLELVSEVGADIVFHPAVEDMYPG
                     ELGIDVKVGPLADVLEGAKRPGHFDGVVTVVNKLFNIVMPDYAYFGKKDAQQLAIVEQ
                     MVKDFNHAVEIIGIDIVREADGLAKSSRNVYLTEQERQEAVHLSKSLLLAQALYQDGE
                     RQSKVIIDRVTEYLESHISGRIEEVAVYSYPQLVEQHEITGRIFISLAVKFSKARLID
                     NIIIGAE"
     gene            complement(8391..9209)
                     /gene="panB"
                     /locus_tag="O0293_000937"
     CDS             complement(8391..9209)
                     /gene="panB"
                     /locus_tag="O0293_000937"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCY0279831.1"
                     /translation="MKTVSQLIDMKQKQTKISMVTAYDFPSAKQVEAAGIDMILVGDS
                     LGMTVLGYESTVQVTLADMIHHGRAVRRGAPNTFVVVDMPIGAVGISMTQDLNHALKL
                     YQETNANAIKAEGAHITPFIEKATAIGIPVVAHLGLTPQSVGVMGYKLQGATKEAAEQ
                     LILDAKNVEQAGAVALVLEAIPNDLAEEISKHLTIPVIGIGAGKGTDGQVLVYHDMLN
                     YGVEHKAKFVKQFADFSVGVDGLKQYDQEVKSGAFPSEEYTYKKKIMNEVNNND"
     gene            9282..10142
                     /locus_tag="O0293_000938"
     CDS             9282..10142
                     /locus_tag="O0293_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCY0279832.1"
                     /translation="MLSVAIIGPGAVGTTIAYELQQSLPHTTLIGRHAKTITYYTVPH
                     APAQDIVVKGYEDVTNTFDVIIIAVKTHQLDAVIPHLTHLAHEDTLIILAQNGYGQLE
                     HISFKNVCQAVVYISGQKKGDVVTHFRDYQLRIQDNALTRQFRDLVQDSQIDIVLEAN
                     IQQAIWYKLLVNLGINSITALGRQTVAIMHNPEIRTLCRQLLLDGCRVAQAEGLNFSE
                     QTVDTIMTIYQGYPDEMGTSMYYDIAHQQPLEVEAIQGFIYRRAREHNLDTPYLDTIY
                     SFLRAYQQNM"
     gene            complement(10228..10932)
                     /gene="budA"
                     /locus_tag="O0293_000939"
     CDS             complement(10228..10932)
                     /gene="budA"
                     /locus_tag="O0293_000939"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCY0279833.1"
                     /translation="MTNVLYQHGTLGTLMAGLLEGTATINELLEHGNLGIATLTGSDG
                     EVIFLDGKAYHANEHKEFIELKGDEKVPYASITNFKASKTFPLQQLSQDDVFAQIKNE
                     MLSENLFSAVKIYGTFKHMHVRMMPAQQPPYTRLIDSARRQPEEKRQDIRGAIVGFFT
                     PELFHGVGSAGFHIHFADDERAYGGHVLDFEVDDVVVEIQNFETFQQHFPVNNETFVK
                     AKIDYKDVAEEIREAE"
     gene            complement(11210..12169)
                     /locus_tag="O0293_000940"
     CDS             complement(11210..12169)
                     /locus_tag="O0293_000940"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCY0279834.1"
                     /translation="MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVK
                     ADVQDLNHGTVHSPSPVDVKAGEYEDCKDADLVVITAGAPQKPGETRLQLVEKNTKIM
                     KSIVKSVMDSGFDGYFLIAANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLIS
                     QELGVAPSSVDASIIGEHGDTELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTR
                     DAAYEIIQAKGSTYYGIALALMRISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPT
                     LVNQHGAVKIYEMPLSAEEQALFDKSVKTLEDTFDSIKYLLED"
     gene            12856..14304
                     /locus_tag="O0293_000941"
     CDS             12856..14304
                     /locus_tag="O0293_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0279835.1"
                     /translation="MGSFFNKIARKEDPAIYQNKDGHLKRTLRVRDFLALGVGTIVST
                     SIFTLPGIVAAEHAGPAVALSFLLAAIVAGLVAFTYAEMAAAMPFAGSAYSWVNVLFG
                     EFFGWVAGWALLAEYFIAVAFVASGFSANLRGLVKPIGIELPAALSNPFGTNGGFIDI
                     IAAIVILLTALLLSRGMSEAARMENILVILKVLAIILFVIVGLTAINVSNYVPFIPEH
                     KVTATGDFGGWQGIYAGVSMIFLAYIGFDSIAANSAEALDPQKTMPRGILGSLSVAIV
                     LFIAVALVLVGMFHYSQYANNAEPVGWALRQSGHGVVAAIVQAISVIGMFTALIGMML
                     AGSRLLYSFGRDGLLPSWLSHLNDKHLPNRALVILTIIGVLIGSMFPFAFLAQLISAG
                     TLVAFMFVSLAMYRLRKREGKDLPIPAFKLPLYPVLPAITFVLVLLVFWGLGFEAKLY
                     TLIWFIVGIILYLSYGLRHSKKNDVAEYHPPK"
     gene            complement(14385..15722)
                     /locus_tag="O0293_000942"
     CDS             complement(14385..15722)
                     /locus_tag="O0293_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HCY0279836.1"
                     /translation="MSKAHQLIQEDEHYFAKSGRIKYYPLVIDHGYGATLVDIEGKTY
                     IDLLSSASSQNVGHAPREVTEAIKAQVDKFIHYTPAYMYHEPLVRLAKKLCEIAPGDF
                     EKRVTFGLTGSDANDGIIKFARAYTGRPYIISFTNAYHGSTFGSLSMSAISLNMRKHY
                     GPLLNGFYHIPFPDKYRGMYEQPQANSVEEYLAPLKEMFAKYVPADEVACIVIETIQG
                     DGGLLEPVPGYFEALEKICREHGILIAVDDIQQGFGRTGTWSSVSHFNFTPDLITFGK
                     SLAGGMPMSAIVGRKEIMNCLEAPAHLFTTGANPVSCEAALATIQMIEDQSLLQASAE
                     KGEYVRKRMDQWVSKYNSVGDVRGKGLSIGIDIVSDKKLKTRDASAALKICNYCFEHG
                     VVIIAVAGNVLRFQPPLVITYEQLDTALNTIEDALTALEAGNLDQYDISGQGW"
     gene            15980..16396
                     /locus_tag="O0293_000943"
     CDS             15980..16396
                     /locus_tag="O0293_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279837.1"
                     /translation="MRLKVLFHFIAAIFISFMLLWMTMLFDLISNQSHLKALLLNLDF
                     LIPSDNTPYILEIICHLLIGSVIYFVFVLLFHTSKRLYYLCYIPLFFLFIALYPFLVF
                     IAQRPIFQFSVTELIGWIITHIFFMSLMALVIPRIK"
     gene            16519..17409
                     /locus_tag="O0293_000944"
     CDS             16519..17409
                     /locus_tag="O0293_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002478556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HCY0279838.1"
                     /translation="MNKEQLEKMKNGKGFIAALDQSGGSTPKALKEYGVNEDQYSNED
                     EMFQLVHDMRTRVVTSPSFSPDKILGAILFEQTMDREVESKYTADYLADKGVVPFLKV
                     DKGLAEEQNGVQLMKPIDNLDNLLDRANERHIFGTKMRSNILELNEQGIKDVVEQQFE
                     VAKQIIAKGLVPIIEPEVNINAKDKAEIEKVLKAELKKGLDSLNADQLVMLKLTIPTE
                     PNLYKELAEHPNVVRVVVLSGGYSREKANELLKDNAELIASFSRALASDLRADQSKEE
                     FDKALGDAVESIYDASVNKN"
     gene            complement(17602..19098)
                     /gene="mqo"
                     /locus_tag="O0293_000945"
     CDS             complement(17602..19098)
                     /gene="mqo"
                     /locus_tag="O0293_000945"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCY0279839.1"
                     /translation="MAKSNSKDIVLIGAGVLSTTFGSMLKEIEPDWNIHVYERLDRPA
                     IESSNERNNAGTGHAALCELNYTVLQPDGSIDIEKAKVINEEFEISKQFWGHLVKSGS
                     IENPREFINPLPHISYVRGKNNVKFLKDRYEAMKAFPMFDNIEYTEDIEVMKKWIPLM
                     MKGREDNPGIMAASKIDEGTDVNFGELTRKMAKSIEAHPNATVQFNHEVVDFEQLSNG
                     QWEVTVKNRLTGEKFKQVTDYVFIGAGGGAIPLLQKTGIPESKHLGGFPISGQFLACT
                     NPQVIEQHDAKVYGKEPPGTPPMTVPHLDTRYIDGQRTLLFGPFANVGPKFLKNGSNL
                     DLFKSVKTYNITTLLAAAVKNLPLIKYSFDQVIMTKEGCMNHLRTFYPEARNEDWQLY
                     TAGKRVQVIKDTPEHGKGFIQFGTEVVNSQDHTVIALLGESPGASTSVSVALEVLERN
                     FPEYKTEWAPKIKKMIPSYGESLIEDEKLMRKIRKQTSKDLELGYYEN"
BASE COUNT         6139 a         3568 c         3043 g         6534 t
ORIGIN      
        1 aaatattgtt ttatgattat attcaaaatt attgtatcaa taaatatgta tcattcctag
       61 aattttattg aacaaaaagt tataaaatca cttttattgt aactcaacct tgttttacat
      121 tacagaaatt aacaatagcg attaaaattt tattttccaa caataaaaac gcacaaatat
      181 ttttattgag gcagctgtat cgcttctcat tgtaaaaata tctgtgcata ttatctattt
      241 atctcaatct aacgtcttaa ctaacttgtt gtatcttgta aaatcttatc catagaatgc
      301 gcaacactat cacggtacat ctcaccaaat ttcaataaat ggaccatcaa taaatataaa
      361 cgataaaatt caagtctata ggatgcacct tttgcgagtg gataatgttt attatacgca
      421 tcataaaatt cgctcgtaaa accaccaaat accgttgtaa taccgatatc gaattctctg
      481 tcaccatata atggcgctgg atcaaataac gccggacgac catcttgtaa gaacatataa
      541 ttaccacccc ataggtcacc atgtaataaa gacggtttac tttgatgctt ttctaattcc
      601 gccacaattt gacgtcgcac tttgtcatat actttgatat cgttggcatc ccatagccct
      661 ctatttagca actcatcttt caaatggtcc aagcgtttgt cgacaaaaat ggtacaccaa
      721 tcgtcttgcc aatgattatc aaaagaaata tcgccacctt cataaggtaa tgagaagcca
      781 aatttgcctt cttcttgttg ctgactgtgt aattgagcta cgagttgccc taattggcgt
      841 tgactccctg aagccccttc ttctaaatac gtcatcacta aatacgcatc accgttaacc
      901 tcgccacttg caattactct aggtgccgtg atacctgcac gttcaaattc atttaaacct
      961 gcaatttctg cagcataaaa tgattcttta cgtccacgtt ggacaagtaa gaaaaatgta
     1021 tccgtatctg tttcgactcg atatgcttcg tttacatcac caccactcac tggtgaaatc
     1081 tctttaatat cttttaaagg taaatgctct aaccattgtt cattcataca aacaccttcc
     1141 aatctactta ctttatacta taatattgcc taattaggat gatttaatct taaaaacgtt
     1201 attcaaactt gcgtcaattt tcaaaacttt ttagttttaa ctaggaaatt atgtatggaa
     1261 atgctagtct attaaaagaa tatatttcac atatattaag ttgaggatag taagcctcaa
     1321 atataatgaa ataggagcat actcatgtac aaattaatta aacctttctt attcaaaatc
     1381 gaacccgaaa aagcacacgg actaactatc gatgcattaa aaacgttaca aaagtttccg
     1441 gttttattcc cagtcgtcga taaactattt acttataaga atccaacgtt atcacaaacg
     1501 atacaaggta atacgtacga caatccaatt ggcttagcag ctggtttcga caaatcttgc
     1561 gaagtaccaa aagcattgga acaccttgga ttcggtgctt tagaattagg tggtatcaca
     1621 cctaaacctc aaccgggtaa ccctcaacca cgcatgttta gattattaga agatgacgcc
     1681 ttgataaatc gaatgggctt caataatatt ggtatgaaca aagcactaag tcatttgcgt
     1741 aaaaatgctt atcaagtacc tgttggtatc aatgttggtg tgaataaaat gacaccttat
     1801 gaagcgcgtt atcaagatta tataaaggtt attgatacgt ttaaacacga cgtttcattt
     1861 ttcacagtca acatcagttc tccaaatact gaaaatcttc aaaacttcca tgataaagat
     1921 gaattttcaa tgttatgcca agctttaaca gcatttaaaa aacaacatga tgtaacagtg
     1981 ccaatttatt taaaactaac gtctgatatg gatttcgatg gcttaaaagc actattacca
     2041 gcgattactg agacatttga cggtatcatc ttagcaaaca caacgcgaca acgagatggt
     2101 ttaacttctg ctaataaagt cgaagaaggc ggtttgagtg gtcgtccatt atttgaacgt
     2161 aatttaaaat tgattaagta tgcttatcag caaacaaatg gtgaattttt aattataggt
     2221 acaggcggcg tattcagtac tgaagatgca atcaaaatga tgcgtcacgg tgcgtcactt
     2281 attcaaattt attcatcact tgttattgaa ggcccaggtt taactaagaa aatgaacaaa
     2341 ggcatcgcac gttacttaaa agatcatcat tttgacaatg tcagtgatat tataggacta
     2401 gatgcctaat ccaacatagt caaataatac tttaaaactt gctgaatcgt cttattaaaa
     2461 ttatgatata actcaagaag atatatttat caccattttt caaaataatg gcttaaatat
     2521 cacatatcat taaatatagc gttttataac ggaggcgttt gttatgacta aagaaacaga
     2581 taataaaaaa acagtagcga cttctgacaa caaagacaca cagcaacaaa atcaacctga
     2641 agaagatgaa cgtcaaggtt tgaatggtta tcgtaaaaca gatcttgatt tagaaatcga
     2701 gcaagagcta cgtgaaatga tgaaaacagg agaaaatgaa acgaaaaatg actataaaaa
     2761 gtttaaagtg ttttcactta tttcaacact tgtcattgtc attttagcaa ttataagatt
     2821 tgttcataaa atgatgtaat tagagtgaga cattgtttta tgtctcagga tccagttatt
     2881 cattatatct acaatattta cgattatata aataacccga gattttagta tgattcattt
     2941 cactaaaatc tcgggtttct atttgataat ttttaatggg atatggcatg tatacgttct
     3001 tgccttttat ctcatttcca atgattaatc tggatattgt tctaaaaatg ctttcgcttc
     3061 tttattaact gttttaaaat caataccttg ttgcatcgct gcaaagacac atccacaata
     3121 acactgccta aagatattat agtcattaca catttctatg gaacgctcat aacctttact
     3181 tttcttaaaa tcacttggca aatagttcac atcgtatatt ttttggacat ccataccaag
     3241 ttcattgatt aattgtgcgt tctttttagg tgataatgtg attgcactgc caaaataatc
     3301 atagccatgt tctacagctg ctttcgctac aatatccaaa cgcatttcaa aacaagcggt
     3361 acaacgtaag ccgccttctt tttcatcagc taattcttta tccttcacca ttttcacaaa
     3421 cttatgcggt tcataaggtg cttcaatata cttcacattc gcaccagtct tgcgattaaa
     3481 atcttccaca aattgttctt gcactttagc acgtcgtaag tactcatttt tcggatgaat
     3541 atttgaattc gcgaaataaa ttgcaatgtc tgcatattgt gttaaaaact ctaatgtata
     3601 tgtactacaa ggtgcacaac aactatgcaa taagatttta ggtctgattg cttctctttc
     3661 ccactgcccg attaatttct tcaacacctt gtcataatta attttttgat ttttcatttt
     3721 actgataata ggttcagcat taatcatgat ttaacctccc acatttaatc attaacttct
     3781 attatatatg attcatatta aatgtcagtc aaaaaagtta gaaattcatt ttaatgcatt
     3841 tatatttcga aaatccctta tgtatctaaa agcatttttt aaacttgaat tttaaaacac
     3901 taaacaacac atacgtctct gtgtcatttt cattttttgt atgtcatata tatgtttact
     3961 tcatttaaat caatttcatc ttataattta tcgtgtattt tacaaaagat tgacttcaat
     4021 tcatcgtaaa agttatactt ttgccatttt ttaatgtaac atggtgttag taataaaaat
     4081 aatacattga ggtgttttac atgacagcat tattccctta tatcgctttt gaaaattcaa
     4141 aagaagccct tgcatattac gaagaagtat ttggtgcaac tgacgttaaa cgtttagaag
     4201 ttggcgaaga acaagcgtca cattttggta tgactaagga agaagcgcaa gaagcaacta
     4261 tgcatgctga atttgaagtg cttggcgtaa aagtgttatg ttctgattct tttggtcgcg
     4321 ctgacaaaat taataatggc atatcattat taattgatta tgatgttaac aataaggaag
     4381 atgctgataa agttgaagca ttctatgagc aaattaaaga tcattcttca attgaaatag
     4441 aattaccgtt tgctgaccaa ttctggggtg gcaaaatggg cgtctttacc gataaatacg
     4501 gtgttcgttg gatgttacat ggtcaagact atactgcaat ccaacaataa tgttattgat
     4561 aaagatatca tttaataagc ttatgttatt tagcaaggca agccactctc gattcatggc
     4621 ttgccttatt ttattgtata tttttaatta tcgttgctgg cccttgattg acaatcgtat
     4681 taaaatgcct tattaagtca actttgtcta tacggtttgg atcttctacc caatgtctta
     4741 taaaagacaa tcccgcacct gaaacataac tcatgaaata agaaaatggt ataccattaa
     4801 tttgatcatt ttcattttta taacgttgta aatgacacat aattaaatca taaagttttt
     4861 cgtggatatt tgaatcttta cccatacgaa acatcaattg ataaaatgcg atgtcttttt
     4921 ctatcatttc tattaaaacg gtcataattt gatgtatgtt atccgtggat aacttaactg
     4981 ctccatttaa cttctcatca tgaatgaagt ctcttatttc ctccaactgc tggtcctcta
     5041 atttttcaag caaatcatac ttatcataat aatgcgtata aaatgtacta cggttaacat
     5101 cagctaaatc tgcaatttgt tgcacagtaa tctcttctaa ttggtgttga tgtaaaagtt
     5161 caataaatgc atttctcatt gcaacttgtg attttctaat acgtcgatct atagtcattt
     5221 atatcaagtc ctccccaatg attataaacg ttatgttcat tatcccacaa atctccaaca
     5281 ttgatgattg gcacacaatg tttacctgtt taatataggt gatacaaaca aacagaaaaa
     5341 ggtgataaca atgaaccaac atttactagg aaatccaaaa ttaactgtaa ctcatgtcaa
     5401 tgaagttaaa gccggtatta accacatcgt tgtcgacagt gttcaatatg gaaatcaaga
     5461 aatgattatg gaaaaagatg tcactgtgga aatgcgcgat ggcgaaaaat tatatattaa
     5521 tattttcaga ccaaataaag atggcaaatt ccctgtagtt atgtctgcag atacttacgg
     5581 taaagataat aagcctaaaa tcacaaatat gggtgccctt tggccaacat taggtaccat
     5641 tccgacatct agttttacac ctgaagaatc accagaccca ggattttggg tgccaaatga
     5701 ttatgttgta gttaaagttg cattacgcgg tagtgacaaa tccaaaggcg tcttatctcc
     5761 atggtcaaaa agagaagcgg aagattatta cgaagtgatt gaatgggcag caaatcagtc
     5821 atggagtaat ggaaatatcg ggacaaatgg tgtttcttat cttgcggtga ctcaatggtg
     5881 ggtcgcatca ttaaatccac cacatttaaa agcaatgatt ccttgggaag gcttaaatga
     5941 tatgtataga gaagtagcct ttcacggagg tataccagat actggctttt atcgtttctg
     6001 gactcaaggt atttttgcga gatggacaga taatccaaat atcgaagatt tgattcaagc
     6061 acaacaagaa catcctctgt tcgatgattt ttggaaacag cgtcaagtgc cattatcaca
     6121 aattaaaaca cctctactaa catgtgctag ttggtctaca caaggtttgc acaaccgtgg
     6181 ctcttttgaa ggatttaaac aagctgcatc tgaagaaaaa tggctatatg tgcatggacg
     6241 taaagagtgg gaaagttact acgctagaga aaatctcgaa cgccaaaaat cattctttga
     6301 tttttacctt aaagaagaaa ataacgattg gaaagatacg cctcatgtca tttatgaagt
     6361 tagagatcaa ttttataaag gcgaattcaa atcagcgtca gcgttccctt tacctaacgc
     6421 agaatataca ccattgtatt tgaatgctga aaatcacaca ttgaatcatg caaagattag
     6481 tagcgcgcat gtcgcacaat atgactctga agataaacaa caagatgtaa gttttaaata
     6541 tacgtttgac aaagatactg agttagttgg aaacatgaac ttaaaactat gggtaagcac
     6601 taaagactca gatgatatgg atttatttgc aggtattaaa aagttagatc gtcgtggtaa
     6661 tgaagttaac ttccctgatt ttaatcatat tgaaaatggt caagtagcta ctggttggtt
     6721 acgcgtatca catcgtgaat tagatcaaga aaaatcctca atcgcgcaac cttggcataa
     6781 acatgaaaca gaattaaagt tgtcacaaga tgagattgta cctgttgaaa tcgaattgtt
     6841 accttcaggc acgctattta aacaaggcga aacattggaa gttgttgtaa agggtagtga
     6901 aattgtaatt ggtaatagta ctcctggcat gaaaacacgt tatgaacatg aagaaaccgt
     6961 aaataaaggc atgcacatga tttatactgg tggtaaatat gattcacaat taatcattcc
     7021 tatcgttaat tgatatgcag caattacggt cgcttttgat taaaagtgac atagtgatag
     7081 gactgtataa caagagaaag ccacacgctt ggaatcttaa accaaggtgt ggcccttttt
     7141 attattgatg gctatttaat tttataacac tatcgtattt tctttttcat gaatcatttc
     7201 aataatgaca ttatcttcat tcattactgc tactttaggt gcatggtttt taatttcttc
     7261 ttcattcaac tgtgcataag tcatgattat gactacatcg cctacttcaa caagtcttga
     7321 cgctgcaccg tttaaacaaa ttttaccact acctctttca ccagctatta cgtatgtttc
     7381 aaaacgtgca ccattattat tattcacgat ggctactttt tcatttggca agatgtctac
     7441 cgcttccaat atatctgaat caatcgtaat gctacctaca taatttaaat ttgactcagt
     7501 cactcttgct ctatgaattt tagcattcat cattgttctt atcactttat tcagctccaa
     7561 ttattatatt atctattaaa cgcgcttttg aaaatttaac agctaacgag ataaatatgc
     7621 gtccagttat ttcgtgttgt tctactaatt gaggataact ataaacagca acttcttcaa
     7681 tgcgtccact tatatgtgat tcaagatatt cagtaaccct gtctataatt actttacttt
     7741 gacgttcacc gtcttgatac aacgcttgtg ctaatagcaa acttttactt aaatgtaccg
     7801 cttcttgtcg ttcttgctcc gttaaataaa catttcttga acttttcgcc aaaccatctg
     7861 cttctcgaac gatatcaata ccaataattt caacggcatg attgaagtct tttaccattt
     7921 gctcgacaat agccaattgc tgggcatctt ttttaccaaa ataagcataa tccggcataa
     7981 caatattaaa tagcttatta actactgtta ccaccccatc aaaatgccct ggacgcttcg
     8041 ctccttctaa cacatcagct aatgggccta ctttgacatc aatacctaat tcacctggat
     8101 acatatcttc tactgcagga tgaaaaacaa tgtccgctcc tacttctgat actaattcta
     8161 aatctttatc aatttgtctc ggataagcat cgaaatcttc gtttggacca aattgtaatg
     8221 gattaacaaa tacactcaca attgtaatat catttgtact aactgattcg cgtaccatcg
     8281 ttaaatgtcc atcatgtaag gcacccattg ttgggataaa accaatcgtt gtgcctgagc
     8341 gtttggctgc tttaacaatg tgttgcatct cttttaccgt agtaatcagc ttagtcattg
     8401 ttattaacct cattcataat tttcttttta tacgtatatt cttctgatgg aaatgcacca
     8461 gatttaactt cttgatcgta ttgttttaaa ccatccacac caacactaaa atcagcaaat
     8521 tgcttcacaa atttcgcttt atgttcaaca ccataattta acatatcgtg ataaaccaat
     8581 acttgaccat ctgtaccttt tcctgcacca ataccaatga ctggaattgt taagtgcttg
     8641 ctaatttctt ctgctaaatc atttggaatt gcttcaagta ctaacgcaac tgcaccagct
     8701 tgttctacat ttttcgcgtc taaaataagt tgctccgctg cttctttcgt tgcaccttgt
     8761 aatttatacc ccataacgcc aacactttga ggtgttaatc ctaaatgtgc aacaacagga
     8821 ataccaattg ccgttgcttt ttcaataaat ggtgtaatat gcgctccttc tgctttaatt
     8881 gcatttgcat tcgtctcctg ataaagcttt agagcatgat ttaagtcttg tgtcatagag
     8941 atgcctactg caccaatcgg catatcaaca actacaaatg tatttggtgc gcctcttctt
     9001 actgcacgac cgtgatgaat catatctgct aacgtcactt gtacggtact ttcataacct
     9061 aatacagtca taccaagtga atccccaaca agaatcatat caatacccgc tgcttccact
     9121 tgtttagcac ttggaaaatc ataagctgtt accatagaaa ttttagtttg cttttgtttc
     9181 atatctatta attgacttac tgttttcaat gttattcaac ctctttttgc agtattatta
     9241 gaaatagtat aatgaatctt ttatagaaag gaaagaagaa gatgttatca gttgcgatta
     9301 tcggcccagg cgctgtcggt acaactattg cctatgaatt acaacaatca ttgcctcata
     9361 caacgcttat cgggagacac gccaaaacaa taacatatta tactgtacca catgcacctg
     9421 cacaagatat tgttgtgaaa ggttatgaag atgtcacaaa cacatttgat gttattatta
     9481 ttgcagtcaa aacacatcaa cttgatgcag ttattcctca tttaactcat cttgcacatg
     9541 aggacacgct catcatttta gcccaaaatg gttatggtca actcgaacat atttcattta
     9601 aaaacgtatg ccaagcagtt gtctatataa gtggtcaaaa gaaaggcgat gttgttacgc
     9661 acttcagaga ttatcaatta cgtatacaag ataatgcatt aactcgacaa tttagagatt
     9721 tggtccaaga cagtcaaatc gacattgtat tagaagcaaa tattcaacaa gctatttggt
     9781 ataaattgtt agttaacttg ggcattaatt ctatcacagc actcggtaga cagaccgttg
     9841 caatcatgca taatcctgaa atacgtacac tgtgtagaca gctattacta gatggttgtc
     9901 gtgttgcaca ggcagagggg ttaaactttt cagaacaaac tgtcgatact attatgacta
     9961 tatatcaagg ttatcccgac gaaatgggaa cgagcatgta ttacgacatt gcgcatcagc
    10021 aacctcttga agtcgaagcg attcaaggct ttatttatcg acgtgcacgt gaacacaatc
    10081 ttgacacccc atatcttgat actatttata gctttttacg cgcgtatcaa caaaatatgt
    10141 aagcacatat ataatataaa aagaccttaa cgatgcattt agaaccacct gttcctaata
    10201 tcgctaaggt cttttaatta tattagacta ttcagcttct ctaatttctt ctgccacatc
    10261 tttatagtct attttcgctt taacaaacgt ctcgttattt accgggaaat gttgttggaa
    10321 tgtttcaaag ttttgtatct caacgacaac gtcatccact tcaaagtcaa gaacatgtcc
    10381 accataagct ctttcatcat ccgcaaaatg tatatgaaaa ccagcagacc ctacgccatg
    10441 aaataattct ggtgtaaaaa atccaacaat ggcaccacga atatcttgtc ttttttcctc
    10501 aggttgtctg cgtgctgaat caatcaaacg tgtatatggc ggttgctgag caggcatcat
    10561 tcgtacatgc atatgtttaa atgtgccata aattttaaca gccgaaaata aattctcact
    10621 taacatttca tttttaattt gtgcaaatac atcatcttgt gataattgtt gcaatggaaa
    10681 tgtcttactc gctttaaaat tagtaatcga tgcatacggt actttctcat cgccttttaa
    10741 ttctataaat tctttatgct cgttagcatg atatgccttt ccgtctaaaa atattacttc
    10801 gccatcagac cctgttaacg ttgcaatccc taaattccca tgttctaaca attcattaat
    10861 tgtagctgtg ccttctagta agccagccat taacgtacct aatgtaccat gttgatacaa
    10921 gacattcgtc attccaaaac catctcccta tagttattcc tcgttgtcat gctctacttc
    10981 gtgattaatg cagaactact ttcctttata ctcaatgacg cctcaacatt tttattcatg
    11041 tcactaaatt ttcacgagtc ataatacttt attcaattat tattttacgc aaaagtattg
    11101 cctctatcaa tgcttgctaa aaaaatggac ctatcagtgc cacgtgttac tgcaacacta
    11161 atagatcctc ttaccattaa aatatttcta attaatactt aaaataggct tagtcttcta
    11221 ataaatattt aattgaatca aatgtatctt ctaatgtttt aacagattta tcgaacaacg
    11281 cttgttcttc ggcacttaat ggcatttcat aaattttaac tgcgccatgt tggttaacta
    11341 atgttggtac acctaggtaa acgcctttgt gaccaccata ttgaccatct aattgtatag
    11401 aaacatttaa gacattattt tcattattta aaatggcttt tgaaatgcgc atcaatgcta
    11461 atgcaatacc atagtatgtt gaccctttag cttggataat ttcataagca gcgtcacgtg
    11521 tattcacata aatttcttcc gctttagctt cgctaccagt ttgttctttt aatgtgtcat
    11581 atactgaaat acctgctaca tttgcttgtg accaaactgc aagttcagta tcaccatgct
    11641 cgccaataat actagcgtca acacttgaag gtgcaacacc aagttcttgg ctaattaaat
    11701 attgtaaacg tgcactgtcc aatacagtac ctgaaccgat aacacgctct gctggtaatc
    11761 cagtatattc ttttacaaat cttgttaaaa tgtctacagg gtttgccgcg attaagaaat
    11821 atccatcaaa gccactatcc ataacactct taacgatgct cttcataatc ttagtatttt
    11881 tttcaactaa ttgtaaacgt gtttcacctg gcttttgagg tgcaccagct gtaataacaa
    11941 ctaaatctgc atctttacag tcttcgtatt cacctgcttt cacatcaact ggtgaaggac
    12001 tgtggactgt accatggttt aaatcttgaa catctgcttt tactttgtct tttgcaatgt
    12061 caataattac aaattcatca gcaacacctt gcgtaaccat tgcaaaggca tagcttgatc
    12121 ctacagatcc atctccgatt aatacaacct ttttaccaaa tgttttcata attatgtctc
    12181 ctaacctaat tgaatttatt ttacacgtga ctacacgtaa aaatatctat actgaaatta
    12241 aagattaaca attttatttt attataatat tatgctacca aattacaaac ataaaactga
    12301 gatgtaagcg gagtcaaatt acactctatc gccaataaat attgatatct caagctttag
    12361 cacgtaattt gaaatttgtg acaattttta gcattctaaa attgcgtaac gacgctattt
    12421 aaaggcttta acaaactatt gagctttcta tttttttcat tttaaaaagg atggctattc
    12481 gacttaaatg acttactata taacctccta ttttccgcct taccctcatc atatttgatg
    12541 catcatttta tagtcaaaga attacatttc atacaaacta gccaatcaga cataaacatg
    12601 aaattgattt taaaaatata atgtgcaaaa ttgttagaaa attagcaaaa aatagtactt
    12661 attcatttaa tatctgttat aatcagtttt atcatttaaa aaatctatgg atgttatcaa
    12721 gacaagtcat taatacgtaa cataaagttg tgatttttca gaatttttag acaaaatatt
    12781 gattatttgt gctatgatat aacatattaa aaaacaccgt atagaattgt ttatgaatgt
    12841 ttgggaggaa agtttatggg aagttttttc aataaaatag cacgaaaaga ggatccggct
    12901 atctatcaaa ataaagatgg tcatttaaag cgtacacttc gggtgcgtga tttcttagct
    12961 ttaggtgtag gaacaattgt atcgacatct atctttacgc tacctggcat tgttgctgca
    13021 gaacatgcag gaccggccgt tgcgttatca ttcttactcg ctgctattgt tgctggttta
    13081 gttgcattta cttatgcaga aatggctgcc gctatgccat ttgcaggttc agcctattct
    13141 tgggtcaatg tattatttgg tgaatttttt ggatgggttg ccggttgggc tctattagct
    13201 gaatatttta tcgccgtagc ctttgttgca tcaggattct cagcgaattt acgcggactt
    13261 gtgaaaccaa ttggcatcga attacctgca gcattatcaa atccatttgg tacaaatggc
    13321 ggttttatcg atattattgc tgctatcgtt attttattaa ctgcattatt actatcacgt
    13381 ggtatgtcgg aagcagctcg tatggaaaat attttagtta ttttaaaagt attagctatt
    13441 attttatttg tcatcgtagg tttaacagca ataaatgtta gtaactatgt gccatttatt
    13501 ccagaacaca aagtaactgc tacaggtgac tttggtggat ggcaaggcat atatgctggt
    13561 gtttcaatga ttttcttagc gtatatcggt ttcgattcta tcgcagcaaa ctcagcagaa
    13621 gcacttgatc ctcaaaagac aatgcctaga ggtattcttg gttctttaag cgttgctatc
    13681 gtattattta ttgctgtagc acttgtgtta gttggtatgt tccattactc acaatacgca
    13741 aacaatgctg aacctgttgg ttgggcttta cgtcaaagtg gtcatggtgt tgtagcagct
    13801 attgttcaag ctatctctgt tatcggtatg tttacagcat taattggtat gatgttagca
    13861 ggctcacgtt tactttattc atttggacgt gacggcttat taccttcatg gttaagccac
    13921 ttaaacgaca aacatttacc taatcgcgca ctagttatac ttactattat tggtgtttta
    13981 attggttcta tgttcccatt cgcattttta gcacaattaa tttcagcagg tacacttgtt
    14041 gcatttatgt tcgtttcatt agcaatgtat cgtttgagaa agcgtgaagg taaagatcta
    14101 ccaattcctg catttaaatt acctttatat cctgtgttac cagcaattac atttgtttta
    14161 gtattgctag tattctgggg attaggattc gaagcgaaat tatacacttt aatttggttt
    14221 attgttggta tcatcctata tttaagttat ggacttagac attctaagaa aaatgacgta
    14281 gctgaatacc acccaccaaa ataaaaaata aaaaaaatca tttcccatct gattaacatc
    14341 cagatgtgga aatgattttt tattttatat taagataatc gctattacca accttgtcca
    14401 gatatgtcat attgatctaa gttacctgct tccaaagcag tcagtgcatc ttctatagtg
    14461 tttaacgccg tgtctaattg ctcatatgtt attaccaatg gcggttggaa tcgcaacaca
    14521 tttcctgcta cagctataat aactacgcca tgctcaaagc agtaattaca aattttaagt
    14581 gccgcactgg catcacgtgt tttgagtttt ttgtcggaaa caatatcaat accaatgctc
    14641 agacctttac ctctaacatc gcctacacta ttgtatttag atacccattg atccattcgt
    14701 ttcctaacat attccccttt ttccgcacta gcctgaagaa gcgactgatc ttcaatcatt
    14761 tgaattgtgg ctaatgcagc ttcacaacta actggattag cacctgttgt aaataaatgt
    14821 gctggtgctt ctaaacaatt cataatctct ttgcgtccaa caattgctga cataggcata
    14881 ccacctgcta aggattttcc gaaagtgatt aaatcaggcg taaaattaaa atgcgagact
    14941 gaactccatg tacctgttct cccaaaacct tgttgaatat catcgacagc gattaaaata
    15001 ccatgttcac gacaaatctt ttctaacgct tcaaaatacc ctggaactgg ttctaaaagt
    15061 ccaccatcgc cttgtatcgt ttcaataaca atacatgcta cttcgtcagc aggtacatac
    15121 ttcgcaaaca tttcttttaa gggtgctaaa tattcttcta ctgaattagc ttgtggctgc
    15181 tcgtacatac cacgatattt atctggaaac ggaatatgat aaaaaccatt caataacgga
    15241 ccataatgtt tgcgcatatt taaactaata gctgacatag acaatgagcc aaaagttgaa
    15301 ccatgatatg cattagtgaa actaatgata taaggacgcc ctgtatatgc tctggcaaat
    15361 ttaatgatgc catcatttgc gtctgatccg gttaatccga aggtcactct tttttcaaaa
    15421 tctccaggtg caatctcaca aagcttctta gctaaacgta ccaacggttc atgatacata
    15481 tatgctggtg tataatgaat aaatttatca acttgtgcct ttatcgcttc tgttacttct
    15541 ctaggtgcat gacctacgtt ttgagaactc gcactcgata acaaatcgat atatgtcttc
    15601 ccctcaatat caaccaatgt tgctccatat ccatgatcaa tcactaacgg ataatattta
    15661 atacgtcctg atttcgcaaa ataatgttca tcctcttgaa ttaattgatg tgctttactc
    15721 atatacattc ccctcccatt gacagtatta atgtgcaagc aaagtggcct ctcttaaatc
    15781 gattataaaa tccgatttaa aatcctgctc aaataccgtc atgcctctat gaatactatc
    15841 caatatacta ataaaattca gatttgtaaa tatattctga taattaagaa ttttatatca
    15901 atctactttg tttatgcgca taattttgtt cgattaggga attgtttact atattccatt
    15961 tgactctgag gtgatacgaa tgcgtttaaa agtattgttc catttcattg ctgctatttt
    16021 catatcattc atgttgctat ggatgaccat gttgtttgac ttaataagca atcaatcaca
    16081 tttaaaagct ttattattga atttagattt tctaattcct tctgacaata caccatatat
    16141 tctagaaata atatgtcact tactaattgg tagcgtcatt tattttgtat ttgtgttgct
    16201 gtttcatact tctaaaaggc tttactatct atgttatatc cctttatttt tcttatttat
    16261 agcattgtat ccctttttag tatttattgc gcaacgtcca atttttcaat ttagtgtgac
    16321 agaattaatc ggttggatta ttacacatat tttctttatg tcattaatgg cgttagtcat
    16381 acctagaata aaataatatt tttaattaaa ctgacaatta tttttcacat tttatacact
    16441 tttcaaggtt tatgacttga gaaatgttct catttttatc atactggagg tgtattgaaa
    16501 agttaaggag tggaaagtat gaataaagag caattagaaa aaatgaaaaa tggaaaaggc
    16561 tttattgccg cattagacca aagtggtggt agtacaccaa aagcacttaa agaatatggt
    16621 gtaaacgaag atcaatatag caatgaagac gaaatgttcc aacttgttca cgatatgcgt
    16681 acacgtgtgg taacttcacc ttcattctca ccagataaaa ttttgggtgc tattcttttc
    16741 gaacaaacaa tggatcgcga agtagaaagc aaatacactg cagattactt agctgataaa
    16801 ggtgttgttc cgttcttaaa agtagacaaa ggtcttgctg aagagcaaaa tggtgttcaa
    16861 ttaatgaaac caatcgacaa cttagacaat ttattagacc gtgcaaacga acgtcacatt
    16921 tttggtacaa aaatgcgttc taacatttta gaattaaatg agcaaggtat caaagacgtt
    16981 gttgaacaac aatttgaagt tgctaaacaa attattgcta aaggtttagt tccaattatc
    17041 gaaccagaag ttaatattaa tgcaaaagac aaagctgaaa ttgaaaaagt attaaaagct
    17101 gaacttaaaa aaggtttaga tagcttaaat gctgatcaat tagtaatgtt gaaattaaca
    17161 attcctactg aaccaaactt atacaaagag ttagctgaac atcctaatgt tgttcgtgtc
    17221 gttgtattat caggtggtta cagcagagaa aaagcaaatg aattacttaa agataacgct
    17281 gaacttatcg caagtttctc acgtgcatta gctagtgatt taagagctga tcaatctaaa
    17341 gaagaattcg ataaagcttt aggtgatgct gtagaatcaa tctacgacgc atctgtaaac
    17401 aaaaactaat ttatacatta aactattttt tgatataaac tactaaaaag gactcataca
    17461 tgtgctttaa aggcattgtg tatgagtctt ttttgattaa agttgatttt gccgtatgta
    17521 ttagattacg taaaaaaatc ggacagctga aaaatcttta agatttctca gttgtccgat
    17581 tttaaatata gctattagaa tttagttttc gtagtaacct aattctaagt cttttgaagt
    17641 ttgtttacgg atttttctca ttaatttttc gtcttcaatt aatgattcac cgtatgatgg
    17701 aatcattttc ttaattttag gtgcccattc agttttgtat tcagggaagt tacgttctaa
    17761 tacttctaac gcaactgaaa ctgaagttga agcccctggt gattcaccta ataatgcaat
    17821 tacagtgtgg tcttgtgagt taaccacttc tgtaccgaat tggatgaatc ctttaccgtg
    17881 ttcaggtgta tctttgataa cttgtacacg tttaccagca gtgtataatt gccaatcttc
    17941 attacgtgct tctggataga aagtacgtaa gtggttcata caaccttctt ttgtcataat
    18001 tacttggtca aatgagtatt taattaaagg taagttttta actgctgctg ctaataaagt
    18061 tgtaatgttg tatgttttaa cagacttgaa taaatctaag ttagaaccat ttttcaagaa
    18121 tttaggtcca acattagcaa atggtccaaa taataatgtt ctttgaccat caatgtaacg
    18181 cgtatctaaa tgaggtacag tcattggtgg tgtaccaggt ggctctttac cataaacttt
    18241 ggcatcgtgt tgttcaataa cttgtgggtt tgtacaagct aagaattgac cactgatagg
    18301 gaatccaccc aaatgtttac tttcagggat acctgttttt tgtaataatg gaattgctcc
    18361 accgccagca ccgatgaata cgtagtcagt tacttgtttg aatttctcac cagttaggcg
    18421 atttttaaca gtaacttccc attgaccatt tgataattgt tcaaaatcaa caacttcatg
    18481 gttaaattgc actgtagcat ttggatgtgc ttcaatgctt ttagccattt tacgtgttaa
    18541 ttcaccgaag tttacatctg taccttcgtc aattttactt gccgccatga taccagggtt
    18601 atcttcacgg cctttcatca tcaatggaat ccattttttc attacttcga tgtcttcagt
    18661 atattcgata ttatcgaaca tagggaaagc tttcatcgct tcgtaacgat cttttaagaa
    18721 tttaacattg tttttacctc taacataact gatgtgtggt aatggattga taaattctct
    18781 tgggttctcg atgctaccgc ttttcactaa gtgaccccag aattgttttg aaatctcaaa
    18841 ctcttcgtta atcactttcg ctttttcgat gtcgatagaa ccatcaggtt gtaaaactgt
    18901 gtagttcaac tcacataatg ctgcatgacc cgtaccagca ttatttcttt cgtttgaact
    18961 ttcgattgca ggacgatcca agcgttcgta aacgtggata ttccagtctg gctcaatttc
    19021 ttttaacatt gaaccaaatg ttgtgctaag tacaccggct ccaattaaaa cgatgtcttt
    19081 actattagac ttagccattg gtttcacctc tccaaaaatt gtaaatgtgt atcatcaata
    19141 tgaaagttac ataaaactga catatttctt taaaatatca acgccattga taacttcctg
    19201 ttttaattga tacgctgtaa caaaatacta tagttagtgc ttacatgtat atgttaaagc
    19261 aagcagtggt aaatgtaaat tata
//
LOCUS       DAKTIO010000036        19224 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.036, whole genome shotgun sequence.
ACCESSION   DAKTIO010000036 DAKTIO010000000
VERSION     DAKTIO010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19224
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.036"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            126..1316
                     /locus_tag="O0293_000946"
     CDS             126..1316
                     /locus_tag="O0293_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HCY0279840.1"
                     /translation="MTKSEKIIELTNHYGAHNYLPLPIVISEAEGVWVKDPEGNKYMD
                     MLSAYSAVNQGHRHPKIIQALKDQADKVTLVSRAFHSDNLGEWYEKICKLAGKDKALP
                     MNTGAEAVETALKAARRWAYDVKGIEPNKAEIIAFNGNFHGRTMAPVSLSSEAEYQRG
                     YGPLLDGFRKVDFGDVDALKAAINENTAAVLVEPIQGEAGINIPPEGYLKAIRELCDE
                     HNVLFIADEIQAGLGRSGKLFATDWDNVKPDVYILGKALGGGVFPISVVLADKEVLDV
                     FTPGSHGSTFGGNPLACAASIAALDVIVDEDLPGRSLELGDYFKEQLKQIDHPSIKEV
                     RGRGLFIGVELNESARPYCEALKEEGLLCKETHDTVIRFAPPLIITKEELDLALEKIR
                     HVFQ"
     gene            1425..2669
                     /locus_tag="O0293_000947"
     CDS             1425..2669
                     /locus_tag="O0293_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HCY0279841.1"
                     /translation="MTENNNLVTSTQGIIKEALHKLGFDEGMYDLIKEPLRMLQVRIP
                     VRMDDGTVKTFTGYRAQHNDAVGPTKGGVRFHPDVDEEEVKALSMWMTLKCGIVNLPY
                     GGGKGGIVCDPRQMSIHEVERLSRGYVRAISQFVGPNKDIPAPDVFTNSQIMAWMMDE
                     YSALDKFNSPGFITGKPIVLGGSHGRDRSTALGVVIAIEQAAKRRNMQIEGAKVVIQG
                     FGNAGSFLAKFLYDLGAKIVGISDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEETI
                     SNKELFELDCDILVPAAISNQITEDNAHDIKASIVVEAANGPTTPEATRILTERGILL
                     VPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVDEKLREKLEAAFDTIYELSQNRKIDM
                     RLAAYIIGIKRTAEAARYRGWA"
     gene            complement(3172..4101)
                     /locus_tag="O0293_000948"
     CDS             complement(3172..4101)
                     /locus_tag="O0293_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCY0279842.1"
                     /translation="MTNSSKSFTKFMAASAVFTMGFLSVPTAGAEQTNQIANKPQAIQ
                     WHTNLTNERFTTIAHRGASGYAPEHTFQAYDKSHNELKASYIEIDLQRTKDGHLVAMH
                     DETVNRTTNGHGKVEDYTLDELKQLDAGSWFNKKYPKYARASYKNAKVPTLDEILERY
                     GPNANYYIETKSPDVYPGMEEQLLASLKKHHLLNNNKLKNGHVMIQSFSDESLKKIHR
                     QNKHVPLVKLVDKGELQQFNDQRLKEIRSYAIGLGPDYTDLTEQNTHHLKDLGFIVHP
                     YTVNEKADMLRLNKYGVDGVFTNFADKYKEVIK"
     gene            complement(4343..5722)
                     /gene="argH"
                     /locus_tag="O0293_000949"
     CDS             complement(4343..5722)
                     /gene="argH"
                     /locus_tag="O0293_000949"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCY0279843.1"
                     /translation="MSNKAWGGRFEVQPEEWVDDFNASITFDQTLIDQDIEGSIAHAT
                     MLANQGIISQQDSEQIIQGLKSIQHDYHQDQIQFSASLEDIHLNIEHELIKRIGDAGG
                     KLHTGRSRNDQVATDMHLYTKKQVQDIIALIKSLQSVIVDIASNNVDTIMPSYTHLQR
                     AQPISFAHHIMTYFWMLQRDQQRFEDSLKRIDINPLGAAALSGTTYPIDRHETTALLN
                     FGSLYENSLDAVSDRDYIIETLHNISLTMVHLSRFAEEIIFWSTDEAKFITLSDAFST
                     GSSIMPQKKNPDMAELIRGKVGRTTGHLMSMLMTLKGLPLAYNKDMQEDKEGLFDAVH
                     TIKGSLRIFEGMIQTMTINKERLNQTVKEDFSNATELADYLVTKNIPFRTAHEIVGKI
                     VLECIQQGHYLLDVPLATYQQHHSSIDADIYDYLQPENCLKRRQSYGSTGQSSVKQQL
                     DVAKQLLSQ"
     gene            complement(5712..6917)
                     /locus_tag="O0293_000950"
     CDS             complement(5712..6917)
                     /locus_tag="O0293_000950"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCY0279844.1"
                     /translation="MKEKIVLAYSGGLDTSVAVQWLIDKGYDVVACCLDVGEGKDLDI
                     VYKKALDMGAVECHIIDATKEFSDEYVSYAIKGNLMYENAYPLVSALSRPLIAKKLVE
                     IAEKTNSVGIAHGCTGKGNDQVRFEVAIKALNPSLKAFAPVREWAWSREEEIDYAIKH
                     NIPVSINHDSPYSIDQNLWGRANECGILEDPYAAPPEDAFDLTNALEETPDTADEIIL
                     TFDKGIPVQIDGKTYELDDLILTLNALAGKHGIGRIDHVENRLVGIKSREIYEAPAAE
                     VILKAHKALETITLTKDVAHFKPIIEKQFAEQLYNGLWFSPLTDSLKLFIDSTQQYVS
                     GDVRIKLFKGNAIVNGRKSPYTLYDEKLATYTKEDAFNQDAAVGFIDIYGLPTQVNAM
                     LHGGYSNEQ"
     gene            7268..8599
                     /locus_tag="O0293_000951"
     CDS             7268..8599
                     /locus_tag="O0293_000951"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCY0279845.1"
                     /translation="MTHIQLDFSKTLEFFGEHELKQQQEIVKSIHKTIHEGTGAGSDF
                     LGWVDLPVDYDKEEFSRIVEASKRIKENSDVLVVIGIGGSYLGARAAIEMLTSSFRNS
                     NEYPEIVFVGNHLSSTYTKELVDYLADKDFSVNVISKSGTTTEPAVAFRLFKQLVEER
                     YGKEEAQKRIFATTDKEKGALKQLATNEGYETFIVPDDVGGRYSVLTAVGLLPIATAG
                     INIEAMMIGAAKAREELSSDKLEENIAYQYATIRNILYAKGYTTEMLINYEPSMQYFN
                     EWWKQLFGESEGKDFKGIYPSSANYTTDLHSLGQYVQEGRRFLFETVVKVNHPKYDIT
                     IEKDSDDLDGLNYLAGKTIDEVNTKAFEGTLLAHTDGGVPNMVVNIPQLDEETFGYVV
                     YFFELACAMSGYQLGVNPFNQPGVEAYKQNMFALLGKPGFEDLKKELEERL"
     gene            8924..9499
                     /locus_tag="O0293_000952"
     CDS             8924..9499
                     /locus_tag="O0293_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCY0279846.1"
                     /translation="MSFHQVEEWFEIFRQFGYLPGFILLYIRAIIPVFPLALYILINI
                     QAYGPILGILISWLGLISGTFTVYLICKRLVNTERMQRIKQRTAVQRLISFIDRQGLI
                     PLFILLCFPFTPNTLINFVASLSHIRPKYYFIVLASSKLVSTIILGYLGKEITTILTH
                     PLRGILMLVVLLVFWIVGKKLEQHFMGSKKE"
     gene            9504..10028
                     /gene="lepB"
                     /locus_tag="O0293_000953"
     CDS             9504..10028
                     /gene="lepB"
                     /locus_tag="O0293_000953"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCY0279847.1"
                     /translation="MKKVVKYLISLILAIIIVLFVQTFVIVGHVIPNNDMSPTLNKGD
                     RVIVNKIKVTFNQLNNGDIITYRRGNEIYTSRIIAKPGQSMAFRQGQLYRDDRPVDAS
                     YAKNRKIKDFSLRNFKELDGDIIPPNNFVVLNDQDNNKHDSRQFGLIDKKDIIGNVSL
                     RYYPFSKWTVQFKS"
     gene            10044..10619
                     /gene="lepB"
                     /locus_tag="O0293_000954"
     CDS             10044..10619
                     /gene="lepB"
                     /locus_tag="O0293_000954"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCY0279848.1"
                     /translation="MKKEILEWIISIAVAFVILFIVGKFIVTPYTIKGESMDPTLKDG
                     ERVAVNIVGYKTGGLEKGNVVVFHANKNDDYVKRVIGVPGDKVEYKNDTLYVNGKKQD
                     EPYLNYNLKHKQGDYITGTFQVKDLPNANPKSNVIPKGKYLVLGDNREVSKDSRAFGL
                     IDEDQIVGKVSFRFWPFSEFKHNFNPENTKN"
     gene            10779..14255
                     /gene="addB"
                     /locus_tag="O0293_000955"
     CDS             10779..14255
                     /gene="addB"
                     /locus_tag="O0293_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HCY0279849.1"
                     /translation="MTLHAYLGRAGTGKSTKMLTEIKQKMKADPLGDPIILIAPTQST
                     FQLEQAFVNDPELNGSLRTEVLHFERLSHRIFQEVGSYSEQKLSKAATEMMIYNIVQE
                     QQKYLKLYQSQAKYYGFSEKLTEQIQDFKKYAVTPEHLEHFIADKNMQTRTKNKLEDI
                     ALIYREFEQRIQNEFITGEDSLQYFIDCMPKSEWLKRADIYIDGFHNFSTIEYLIIKG
                     LIKYAKSVTIILTTDGNHDQFSLFRKPSEVLRHIEEIANELNISIERQYFNQLYRFNN
                     QDLKHLEQEFDALQINRVACQGHINILESATMREEINEIARRIIVDIRDKQLRYQDIA
                     ILYRDESYAYLFDSILPLYNIPYNIDTKRSMTHHPVMEMIRSLIEVIQSNWQVNPMLR
                     LLKTDVLTASYLKSAYLVDLLENFVLERGIYGKRWLDDELFNVEHFSKMGRKAHKLTE
                     DERNTFEQVVKLKKDVIDKILHFEKQMSQAETVKDFATAFYESMEYFELPNQLMTERD
                     ELDLNGNHEKAEEIDQIWNGLIQILDDLVLVFGDEPMSMERFLEVFDIGLEQLEFVMI
                     PQTLDQVSIGTMDLAKVDNKQHVYLVGMNDGTMPQPVTASSLITDEEKKYFEQQANVE
                     LSPTSDILQMDEAFVCYVAMTRAKGDVTFSYSLMGSSGDDKEISPFLNQIQSLFNQLE
                     ITNIPQYHEVNPLSLMQHAKQTKITLFEALRAWLYDEIVADSWLDAYQVIRDSDHLNQ
                     GLDYLMSALTFDNETVKLGETLSKDLYGKEINASVSRFEGYQQCPFKHYASHGLKLNE
                     RTKYELQNFDLGDIFHSVLKYISERINGDFKQLDLKKIRQLTNEALEEILPKVQFNLL
                     NSSAYYRYLSRRIGAIVETTLSALKYQGTYSKFMPKHFETSFRRKPRTNDELIAQTLT
                     TTQGIPINIRGQIDRIDTYTKNDTSFVNIIDYKSSEGSATLDLTKVYYGMQMQMMTYM
                     DIVLQNKQRLGLTDIVKPGGLLYFHVHEPRIKFKSWSDIDEDKLEQDLIKKFKLSGLV
                     NADQTVIDALDIRLEPKFTSDIVPVGLNKDGSLSKRGSQVADEATIYKFIQHNKENFI
                     ETASNIMDGHTEVAPLKYKQKLPCAFCSYQSVCHVDGMIDSKRYRTVDETINPIEAIQ
                     NININDEFGGEQ"
     gene            14256..17909
                     /gene="addA"
                     /locus_tag="O0293_000956"
     CDS             14256..17909
                     /gene="addA"
                     /locus_tag="O0293_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HCY0279850.1"
                     /translation="MTIPEKPQGVIWTDAQWQSIYATGQDVLVAAAAGSGKTAVLVER
                     IIQKILRDGIDVDRLLVVTFTNLSAREMKHRVDQRIQEASIADPANAHLKNQRIKIHQ
                     AQISTLHSFCLKLIQQHYDVLNIDPNFRTSSEAENILLLEQTIDEVIEQHYDILDPAF
                     IELTEQLSSDRSDDQFRMIIKQLYFFSVANPNPKNWLDQLVTPYEEEAQQAQLIQLLT
                     DLSKVFITAAYDALNKAYDLFSMMDSVDKHLAVIEDERRLMGRVLEGGFIDIPYLTGH
                     EFGARLPNVTAKIKEANEMMVDALEDAKLQYKKYKSLIDKVKSDYFSREADDLKADMQ
                     QLAPRVKYLARIVKDVMSEFNRKKRSKNILDFSDYEHFALQILTNEDGSPSEIAESYR
                     QHFQEILVDEYQDTNRVQEKILSCIKTGDEHNGNLFMVGDVKQSIYKFRQADPSLFIE
                     KYQRFTIDGDGTGRRIDLSQNFRSRKEVLSTTNYIFKHMMDEQVGEVKYDEAAQLYYG
                     APYDESDHPVNLKVLVEADQEHSDLTGSEQEAHFIVEQVKDILEHQKVYDMKTGSYRS
                     ATYKDIVILERSFGQARNLQQAFKNEDIPFHVNSREGYFEQTEVRLVLSFLRAIDNPL
                     QDIYLVGLMRSVIYQFKEDELAQIRILSPNDDYFYQSIVNYINDEAADAILVDKLKMF
                     LSDIQSYQQYSKDHPVYQLIDKFYNDHYVIQYFSGLIGGRGRRANLYGLFNKAIEFEN
                     SSFRGLYQFIRFIDELIERGKDFGEENVVGPNDNVVRMMTIHSSKGLEFPFVIYSGLS
                     KDFNKRDLKQPVILNQQFGLGMDYFDVDKEMAFPSLASVAYRAVAEKELVSEEMRLVY
                     VALTRAKEQLYLIGRVKNDKSLLELEQLSISGEHIAVNERLTSPNPFHLIYSILSKHQ
                     SASIPDDLKFEKDIAQIEDSSRPNVNISIVYFEDVSTETILDNDEYRSVNQLETMQNG
                     NEDVKAQIKHQLDYRYPYVNDTKKPSKQSVSELKRQYETEESGTSYERVRQYRIGFST
                     YERPKFLSEQGKRKANEIGTLMHTVMQHLPFKKERISEVELHQYIDGLIDKHIIEADA
                     KKDIRMDEIMTFINSELYSIIAEAEQVYRELPFVVNQALVDQLPQGDEDVSIIQGMID
                     LIFVKDGVHYFVDYKTDAFNRRRGMTDEEIGTQLKNKYKIQMKYYQNTLQTILNKEVK
                     GYLYFFKFGTLQL"
     gene            18075..18977
                     /locus_tag="O0293_000957"
     CDS             18075..18977
                     /locus_tag="O0293_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCY0279851.1"
                     /translation="MKFLSFKYNDKTSYGVKVKREDAVWDLTQVFADFAEGDFHPKTL
                     LAGLQQNHTLDFQEQVRKAVVAAEDSGKAEDYKISFNDIEFLPPVTPPNNVIAFGRNY
                     KDHANELNHEVEKLYVFTKAASSLTGDNATIPNHKDITDQLDYEGELGIVIGKSGEKI
                     PKALALDYVYGYTIINDITDRKAQSEQDQAFLSKSLTGGCPMGPYIVTKDELPLPENV
                     NIVTKVNNEIRQDGNTGEMILKIDELIEEISKYVALHPGDIIATGTPAGVGAGMQPPK
                     FLQPGDEVKVTIDNIGTLTTYIAK"
BASE COUNT         6679 a         2845 c         3517 g         6183 t
ORIGIN      
        1 atttaatgaa acttagtgat tatttcatct tttttggtgg aaaagtatct gctttgaaaa
       61 ctaatgtaat tatttggata atgtttcatg tataatagtt ataaatgatg aaaaggggag
      121 atatgatgac taaatctgaa aaaattattg agttaacaaa tcattacgga gcacataatt
      181 atttaccatt gccaattgtc atttcagaag ctgaaggggt atgggttaaa gatcctgaag
      241 gcaataaata tatggatatg ttatctgcat attccgctgt taaccaaggt catagacatc
      301 cgaaaattat tcaagcatta aaagatcaag ctgataaagt gactttagtt tcacgtgctt
      361 ttcatagtga taacttaggt gaatggtacg aaaaaatttg taaactggca ggtaaagata
      421 aagctttacc aatgaataca ggtgctgaag cagtagaaac agctttgaaa gcagcacgac
      481 gctgggcata cgatgttaaa ggaattgagc caaataaagc agaaatcatt gcatttaatg
      541 gtaacttcca tggtcgaaca atggcgccag tttcattatc ttcagaagca gaataccaac
      601 gtggttatgg tccgttatta gatggattta gaaaagttga ttttggagat gtagatgcat
      661 tgaaagctgc aattaatgaa aatactgcag cagttttagt agaaccaatt caaggtgaag
      721 cgggtataaa tataccgcca gaaggatatt tgaaagcaat tagagaatta tgtgatgaac
      781 ataatgtctt atttattgct gacgaaatcc aagcaggatt aggtcgttcg ggtaaattat
      841 ttgctacgga ttgggataat gtaaaacctg atgtctatat tttaggtaaa gcactaggtg
      901 gtggagtctt cccaatttct gttgtattag cagataaaga agtattagat gtctttacac
      961 ctggctcaca tggttcaaca tttggtggta atccacttgc ttgtgctgca tcaattgctg
     1021 cattagatgt tatcgttgat gaggatttac caggccgctc tttagaatta ggagattatt
     1081 ttaaagaaca attaaagcaa attgatcatc catcaattaa agaagtccgt ggacgtggtt
     1141 tgtttatagg tgtggaactt aatgaaagtg ctagaccata ttgtgaagct ttgaaagaag
     1201 aaggcttatt atgtaaagaa acgcatgata ctgtcattcg ttttgcacca ccattaatta
     1261 ttactaaaga agaattggac cttgcacttg aaaaaataag acatgtattt caataatgtc
     1321 aaaaagttgt aacaatcagt ttgtaagggc tttaaaaagg aatacgttat gttacaatat
     1381 gtttgaaagc gaaatcatta ggtaaagaag aggcgaaaag gatcatgact gagaacaata
     1441 atttagtaac ttctactcaa ggaattatta aagaagcatt gcataaattg ggatttgacg
     1501 aaggaatgta cgatttaatt aaagaacctt taagaatgtt acaagtgcgt atccctgtac
     1561 gaatggatga tggcacagta aaaacattca caggttaccg tgcgcaacat aatgatgctg
     1621 ttggaccaac aaaagggggc gtgcgtttcc acccagatgt tgatgaagaa gaagtaaaag
     1681 cattatcaat gtggatgact ttgaaatgtg gcattgtaaa cttaccatac ggtggtggta
     1741 agggtggtat cgtttgtgat ccacgtcaaa tgagcattca tgaagttgaa cgtttatcac
     1801 gcggatatgt aagagcaatt tcacaattcg taggtccgaa caaagatatt ccagcaccag
     1861 atgtatttac aaactcacaa attatggctt ggatgatgga tgaatatagt gcattagata
     1921 aatttaattc accaggtttc atcacaggta aaccaattgt attgggtggt tctcatggac
     1981 gcgacagatc aactgcacta ggtgtagtta ttgcaattga acaagctgca aaacgtcgta
     2041 atatgcaaat tgaaggtgcc aaggttgtta ttcaaggttt cggtaatgcc ggaagtttct
     2101 tagctaaatt cttatatgat ttaggtgcaa aaattgtagg tatctctgat gcttacggtg
     2161 cattacacga tccaaatggc ttagatatag attatttatt agaccgtcgt gatagttttg
     2221 gtacggtaac aaatttattt gaagaaacaa tctcaaataa agaattgttt gaattagatt
     2281 gtgacatttt agtaccagcg gctatttcaa accaaattac agaagacaat gcacatgata
     2341 ttaaagctag tatcgttgtt gaagctgcta atggacctac aacaccagaa gcaacacgta
     2401 ttttaactga acgtggtata ttattagttc cagacgtatt agcaagtgct ggtggtgtaa
     2461 cggtttctta cttcgaatgg gtacaaaata atcaaggtta ttattggtct gaagaagaag
     2521 ttgatgaaaa gctacgtgaa aaattagaag cggcatttga tacgatttac gaattgtctc
     2581 aaaaccgaaa aatagatatg agacttgcag catatatcat aggtattaaa cgtacagcag
     2641 aagcagctag atatcgtggt tgggcataat taattatcat atgtgattta acgagcttgg
     2701 gacagaaaac aaagccctaa gctcgttaat tttattttag cagtagttga ctgtaaaaca
     2761 atgcccgtgt aacacgctct tttcaaaaat agtcggggcc ccaacacaga ggctggtgga
     2821 aagtcagctt acaatagtgt gcaagttggc ggggccccaa cacagaggct ggcggaaagt
     2881 cagcttacaa tagtgtgcaa gttggggtgg agccccgaca cagagaaatt agctcctcaa
     2941 tttctacaga caatgcaagt tggcggggcc ccaacacaga agctggcgaa aagtcagctt
     3001 acaatagtgt gcaagttggg gtgggacgac gaaataaatt ttgcgaaaat atcatttctg
     3061 tcccactccc aaaaagacag cagtaggata atttcaattt ggaaatatct tactgctgtt
     3121 ttttatagta atagctatat ttttgtattt atgttttcta gtttaacatt actacttaat
     3181 gacttcttta tatttatcag cgaaatttgt aaagacacca tcaacgccat atttatttaa
     3241 tcgtaacata tcagcttttt cattcactgt ataaggatgt actataaatc ctaagtcttt
     3301 taaatgatgg gtattttgtt cagttaaatc tgtataatca ggacctaatc caatcgcata
     3361 agagcgtatc tcttttaagc gttggtcgtt aaattgttgt agttcacctt tatcaactaa
     3421 ttttactaat ggcacatgct tattttgacg atgaattttc tttaaacttt cgtcagaaaa
     3481 tgattgaatc attacatgtc cattttttaa tttattgtta tttaaaaggt gatgcttttt
     3541 caatgaagct aataattgtt cttccattcc tgggtataca tcaggtgact ttgtttcaat
     3601 ataatagttt gcattcgggc cataacgttc taaaatttca tctaaagtgg gtactttagc
     3661 atttttataa cttgctcttg cgtattttgg atatttttta ttaaaccaac ttcctgcatc
     3721 taactgtttt aattcatcaa gggtataatc ctcaacttta ccgtgtccat ttgttgtacg
     3781 gttaacagtt tcatcatgca tagcaactaa atggccatct ttggtacgtt gtaaatcaat
     3841 ttcgatataa gatgctttta actcattatg actcttatca tatgcttgaa acgtatgctc
     3901 gggtgcatag ccacttgcgc cacgatgtgc gatagtagtg aatcgctcat tcgttaaatt
     3961 tgtatgccat tgaatagcct gaggtttatt tgcaatttga tttgtttgtt cagcgccagc
     4021 agtaggtact gataaaaatc ccatagtaaa aacagcagaa gcagccataa atttagtgaa
     4081 gcttttcgaa gagttagtca tagtctttat cctttcatct ctttatttgt agtctaacaa
     4141 ttttaaaata ctacaaaaac ctgcttgtga aaacgcttta cacatttctt ttacaattta
     4201 taacattttt gtaatataaa tttaaaagaa ttatcaaaca ttttaggaat cttaaaaatg
     4261 tgatgttcaa caagacgaag agagcattaa aatacccaca atgtaatttt aatagacatt
     4321 gtgggtaggt agattaacgt atttattgtg atagtaattg tttagcaaca tcaagttgtt
     4381 gtttgaccga tgattgacct gttgaaccgt aactttgacg tcgttttaaa caattttcag
     4441 gctgcaaata atcgtaaata tcggcatcaa tactagaatg atgttgttga tatgttgcta
     4501 aaggaacatc taataaataa tgaccttgtt gtatacattc taagacgatt tttcctacaa
     4561 tttcatgtgc agttctaaat ggaatatttt tagttactaa ataatctgct agttccgttg
     4621 catttgaaaa atcttcttta acagtttgat tgagtcgttc tttattaatt gtcatcgttt
     4681 gaatcatacc ttcgaaaata cgtaaagaac ctttaattgt atggacagca tcgaataaac
     4741 cttctttatc ttcctgcata tctttgttat atgctagagg taatcctttt aaagtcataa
     4801 gcatgctcat taaatgacca gtcgttcgac caactttacc tctaattaat tctgccatat
     4861 caggattttt cttttgtggc ataatagatg agccagttga aaatgcatct gataatgtaa
     4921 tgaatttagc ttcgtctgtg gaccagaaaa taatttcctc tgcaaagcgt gataagtgaa
     4981 ccatcgttaa agaaatatta tgcaatgttt caataatata gtctctgtca ctaacagcat
     5041 ctaggctatt ctcatagaga ctgccaaagt tcaacaatgc tgttgtctcg tgtctatcga
     5101 tagggtatgt ggtaccactt aaggctgctg cacctaaagg attaatatcg attcgtttta
     5161 aactatcttc aaatcgttgt tggtctcgtt gtaacatcca aaaataagtc ataatatgat
     5221 gtgcaaatga aattggctgt gcacgctgta aatgagtata actaggcata attgtatcaa
     5281 cattattgga agcgatgtct acaattacac tttgtaacga cttaattaat gcgatgatat
     5341 cttgcacttg tttcttagtg tacaagtgca tgtctgttgc aacttgatcg tttctactgc
     5401 gtccagtatg caacttacca ccagcatcac cgatacgttt aattaattca tgttcaatat
     5461 ttaaatgaat atcttctaat gatgcactaa attgaatttg atcttgatga taatcatgtt
     5521 gaatagattt tagtccttgt ataatttgtt cgctgtcttg ttgactaata atgccttgat
     5581 tcgcaagcat agttgcatgt gcaatgctgc cttcgatatc ttgatctatg agcgtttgat
     5641 caaaagtaat ggatgcgtta aagtcgtcaa cccactcttc aggttgtact tcaaatctac
     5701 cgccccaagc tttattgctc attgctatag cctccatgca acatcgcatt tacttgagta
     5761 ggtaaaccat agatatcgat aaagccaaca gcagcgtctt gattaaatgc atcttctttt
     5821 gtataagttg ctaatttttc atcatataat gtgtaaggtg attttctacc attcacgatg
     5881 gcattacctt tgaataattt aattctgaca tcaccactta cgtattgctg agtactatca
     5941 ataaataatt tcaagctatc agttaaaggt gagaaccaaa gtccattgta tagttgttca
     6001 gcaaattgct tctcaatgat tggtttaaag tgtgcgacat ctttcgttaa cgtaatcgtt
     6061 tctaatgctt tatgcgcttt taaaataact tctgcagcag gtgcctcata aatttctctt
     6121 gatttgatac ctacaagtct attttctaca tggtcaattc ttccgatacc atgcttacca
     6181 gctaatgcat tcaacgttaa aattaaatcg tctaattcat atgttttgcc atcaatttga
     6241 actgggatgc ctttatcaaa cgttaaaatg atttcatcag cagtatctgg tgtttcttct
     6301 aaagcatttg ttagatcgaa cgcatcctct ggtggcgcag cataaggatc ttctaaaata
     6361 ccacattcat tcgctctgcc ccatagattt tgatcgatag aataaggtga atcatggttg
     6421 attgatacag ggatattatg tttaattgca taatcgattt cttcttcacg actccatgcc
     6481 cactcacgta caggtgcgaa tgctttcaat gatgggttta atgctttaat ggcaacttcg
     6541 aaacgtactt ggtcattccc tttaccagta caaccatgtg caatacctac tgaatttgtt
     6601 ttctcagcaa tctctactaa ttttttagcg attaatggtc ttgataaagc tgaaactaat
     6661 ggatatgcat tttcatacat taaatttcct ttgattgcat aacttacata ctcatcacta
     6721 aattcttttg ttgcatcaat aatatgacat tcaactgctc ccatatctaa agctttttta
     6781 taaacgatgt ctaaatcttt accttcacca acatctaggc aacaagctac aacgtcgtat
     6841 cctttgtcga taagccattg aacggccaca cttgtatcta gtcctcctga atatgctaaa
     6901 acaattttct ctttcataaa attcacctca ttgtagttat ctgaatattt cgtatgaaca
     6961 catgatagca aacttttaat gaaaataaaa ccattatttt acatatttat tcataaaatg
     7021 cgatagagat agttagagtt gtaagtgtaa atgcttattt atcaatgttt aaaagtattt
     7081 taaaaaacat gcaaattgta taaaaaatgt ttgagtgttt gacgccagtt tgaaatgttt
     7141 gttgaaaaat aggtgtgaaa cgtcacataa atatcaattt tattgattaa gtacaaactt
     7201 gtgaattgaa tcgcaaacgt aaacttagta aaattaagat attaaatgaa acaggaggct
     7261 tttatagatg actcatattc aattagattt tagtaaaacg ttagaatttt tcggtgaaca
     7321 cgaattaaaa caacaacaag aaattgttaa atcaattcac aaaacaattc atgaaggtac
     7381 tggtgcaggt agtgacttct taggctgggt tgatttacca gttgattacg acaaagaaga
     7441 attttcaaga attgttgaag catcaaaacg cattaaagaa aattctgatg ttttagtagt
     7501 catcggtatt ggtggttctt acttaggtgc acgtgcagca atcgaaatgt taacgtcatc
     7561 atttagaaac agcaatgaat accctgaaat tgtatttgtt ggtaatcact tatcatcaac
     7621 atatacgaaa gagttagttg attatttagc agacaaagat ttctctgtaa acgttatttc
     7681 taaatctggt acaactacag aaccagcagt tgcatttaga ttgttcaaac aattagttga
     7741 agaaagatac ggtaaagaag aagcacaaaa acgtatattt gcaacaacgg ataaagaaaa
     7801 aggtgcttta aaacagttgg ctacaaacga aggttatgaa acgtttatcg tacctgatga
     7861 tgtaggtgga agatattctg ttttaacagc agtaggatta ttaccaattg caacagctgg
     7921 aattaacatc gaagctatga tgattggtgc tgcaaaagca cgtgaagaat tatcttcaga
     7981 taaattagaa gaaaacattg cataccaata tgcgacaatt cgaaacattt tatatgcaaa
     8041 aggttataca acagaaatgt tgattaacta tgaaccatct atgcaatact ttaatgaatg
     8101 gtggaaacaa ttatttggtg aatcagaagg taaagacttc aaaggtatct atccttcaag
     8161 tgccaactac acaactgatt tacattcttt aggtcaatat gtacaagaag gccgtcgctt
     8221 cttattcgaa acagttgtaa aagtaaatca tcctaaatat gatattacta ttgaaaaaga
     8281 tagtgatgat ctagacggat taaattattt agctggtaaa acaatcgacg aagttaacac
     8341 aaaagcattc gaaggtacat tattagcgca tactgatggt ggtgttccta acatggtagt
     8401 gaacattcca caattagatg aagaaacttt cggttacgtc gtatacttct tcgaacttgc
     8461 ttgtgcaatg agtggatacc aattaggcgt aaatccattt aaccaacctg gtgtagaagc
     8521 atataaacaa aacatgttcg cattattagg taaacctggt tttgaagact tgaaaaaaga
     8581 attagaagaa cgtttataaa atacattact tcaaagatta gtgaagtttg aaaagataga
     8641 actagacgtt aactatttaa agcatatttt cgaggttgtc attacaaatg taaaaatgta
     8701 atgacaacct cgtttttatt tatatgcaag aactaggtta ctagctaatg tgacaagatg
     8761 ttaagagaaa attaaagata aaataatatc tgccttacaa taatattgtt atactactag
     8821 agactgattt attagcatga ttacatgtta atgtttcttt acttagtaat taactttata
     8881 atgtaagaat aattatcttc aaccaaagaa agggattgat gatttgtcgt ttcatcaagt
     8941 agaagaatgg tttgagatat ttcgacagtt tggttattta cctggattta tattgttata
     9001 tattagagct ataattccag tatttccttt agcactctat attttaatta acattcaagc
     9061 ttatggacct attttaggta tattgattag ttggcttgga ttaatttctg gaacatttac
     9121 agtctatttg atctgtaaac gattggtgaa cactgagagg atgcagcgaa ttaaacaacg
     9181 tactgctgtt caacgcttga ttagttttat tgatcgccaa ggattaatcc cattgtttat
     9241 tttactttgt tttcctttta cgccaaatac attaataaat tttgtagcga gtctatctca
     9301 tattagacct aaatattatt tcattgtttt ggcatcatca aagttagttt caacaattat
     9361 tttaggttat ttaggtaagg aaattactac aattttaacg catcctttaa gagggatatt
     9421 aatgttagtt gtgttgcttg tattttggat tgttggaaaa aagttagaac agcattttat
     9481 gggatcgaaa aaggagtgac atcgtgaaaa aagttgtaaa atatttgatt tcattgatac
     9541 ttgctattat cattgtactg ttcgtacaaa cttttgtaat agttggtcat gtcattccga
     9601 ataatgatat gtcaccaacc cttaacaaag gggatcgtgt tattgtaaat aaaattaaag
     9661 ttacatttaa tcaattgaat aatggtgata tcattacata taggcgtggt aacgagatat
     9721 atactagtcg aattattgcc aaacctggtc aatcaatggc gtttcgtcag ggacaattat
     9781 accgtgatga ccgaccggtt gacgcatctt atgccaagaa cagaaaaatt aaagatttta
     9841 gtttgcgcaa ttttaaagaa ttagatggtg atattattcc gccaaacaat tttgttgtgc
     9901 taaatgatca agataataac aagcacgatt caagacaatt tggtttaatc gataaaaagg
     9961 atattattgg taatgttagt ttacgatact atcctttttc aaaatggact gttcagttca
    10021 aatcttaaaa agaggtgtca aaattgaaaa aagaaatatt ggaatggatt atttcaattg
    10081 cagtcgcttt tgtcatttta tttatagtag gtaaatttat tgttacgcca tatacaatta
    10141 aaggtgaatc aatggatcca actttgaaag atggcgagcg agtagctgta aacattgttg
    10201 gatataaaac aggtggtttg gaaaaaggta atgtagttgt cttccatgca aacaaaaatg
    10261 atgactatgt taaacgtgtc atcggtgttc ctggtgataa agtagaatac aaaaatgata
    10321 cattatatgt caatggtaaa aaacaagatg aaccatattt aaactacaat ttaaaacata
    10381 aacaaggtga ttacattact gggactttcc aagttaaaga tttaccgaat gcgaatccta
    10441 aatcaaatgt cattccaaaa ggtaaatatt tagtgcttgg agataatcgt gaagtaagta
    10501 aagatagccg tgcgtttggc ctcattgatg aagaccaaat tgttggtaaa gtttcattta
    10561 ggttctggcc atttagtgaa tttaaacata atttcaatcc tgaaaatact aaaaattaat
    10621 atgaaacaaa tacaacatcg tttgtcggtt ttaatactga taaacgatgt tttattttgt
    10681 tagtaccaca ataaaagcta agttcgaaat gaacttataa taaatcaatc acaatcactt
    10741 tgtgttaaaa tatgtgtcaa aggaagtgag ggtttgtcat gacattacat gcttatttag
    10801 gtagagcggg aacaggtaag tctacgaaaa tgttgaccga aataaaacaa aaaatgaaag
    10861 cagatccgct tggagatcca atcattttaa ttgcgccaac tcaaagtaca tttcaattag
    10921 aacaagcctt tgtcaatgat ccggaattaa atggtagttt aagaacagaa gtgttgcatt
    10981 ttgaacgatt aagtcatcgt attttccaag aagttggtag ttatagcgaa caaaagttat
    11041 ctaaagctgc aacggaaatg atgatttata acattgttca agaacaacaa aagtatttaa
    11101 aactttatca atcacaagca aaatattatg ggtttagtga aaaattaaca gaacaaattc
    11161 aagattttaa aaaatatgca gtaacgcctg aacatttaga acactttatt gctgataaaa
    11221 atatgcaaac tcgaactaaa aataagttag aggatattgc tttaatatac cgtgagttcg
    11281 aacaacgcat tcaaaacgag tttattacgg gtgaggattc attacaatat tttattgatt
    11341 gtatgccgaa atcagagtgg ctaaaacgtg ctgatatata tattgatggt tttcacaact
    11401 tttcaacgat tgagtattta ataatcaaag gattaattaa atatgcgaag agtgtcacaa
    11461 ttatattgac gacagatggt aaccacgatc aatttagttt atttagaaaa ccatcggaag
    11521 tgttacgaca tattgaagaa atagcaaatg aactcaatat ttctattgaa cgtcaatatt
    11581 tcaaccaatt atatcgcttc aataatcaag atttaaagca tcttgaacaa gaatttgatg
    11641 cacttcaaat caatcgagtg gcatgtcaag gtcatatcaa tattttagaa tctgcgacta
    11701 tgagagagga aataaatgaa attgcgcgac gtatcatcgt tgatattcgt gataagcaat
    11761 tacgatatca agatattgct attttatatc gtgatgaatc ttatgcttat ttatttgatt
    11821 ccatattacc gctttataat attccttata atattgatac aaagcgttcg atgacacatc
    11881 atccggtcat ggaaatgatt cgttcattga ttgaagttat tcaatctaat tggcaagtga
    11941 atccaatgct acgcttattg aagactgatg tgttaacggc atcatatcta aaaagtgcat
    12001 acttagttga tttacttgaa aattttgtac ttgaacgtgg tatatacggt aaacgttggt
    12061 tagatgatga gctatttaat gtcgaacatt ttagcaaaat ggggcgtaaa gcgcataaac
    12121 tgaccgaaga tgaacgtaac acatttgaac aagtcgttaa gttaaagaaa gatgtcattg
    12181 ataaaatttt acattttgaa aagcaaatgt cacaagcgga aactgtaaaa gattttgcaa
    12241 ctgcttttta tgaaagtatg gaatatttcg aactgccaaa tcaattgatg acagagcgag
    12301 atgaacttga tttaaatggt aatcatgaaa aggcggagga aattgatcaa atatggaatg
    12361 gcttaattca aatccttgat gacttagttc tagtatttgg agatgaacca atgtcgatgg
    12421 aacgtttctt agaagtattt gatattggtt tagaacaatt agaatttgtt atgattccgc
    12481 aaacattgga ccaagtaagt attggtacga tggatttggc taaagtcgat aataagcaac
    12541 atgtttactt agtaggtatg aatgatggaa cgatgccaca accagtaact gcgtcaagct
    12601 tgattacaga tgaagaaaag aaatactttg aacagcaggc taatgtcgag ttaagtccaa
    12661 catcagatat tttacagatg gatgaagcat ttgtttgtta tgttgctatg actagagcta
    12721 agggagatgt tacattttct tacagtctaa tgggatcaag tggtgatgat aaggagatca
    12781 gcccattttt aaatcaaatt caatcattgt tcaaccaatt ggaaattact aacattcctc
    12841 aataccatga agttaaccca ttgtcactaa tgcaacatgc taagcaaacc aaaattacat
    12901 tatttgaagc attgcgtgct tggttatatg atgaaattgt ggctgatagt tggttagatg
    12961 cttatcaagt aattagagat agcgatcatt taaatcaagg tttagattat ttaatgtcag
    13021 cattaacgtt tgacaatgaa actgtaaaat taggtgaaac gttgtctaaa gatttatatg
    13081 gtaaggaaat caatgccagt gtatcccgtt ttgaaggtta tcaacaatgc ccatttaaac
    13141 actatgcgtc acatggtctg aaactaaatg agcgaacgaa gtatgaactt caaaactttg
    13201 atttaggtga tattttccat tctgttttaa aatatatatc tgaacgtatt aatggcgatt
    13261 ttaaacaatt agacctgaaa aaaataagac aattaacgaa tgaagcattg gaagaaattt
    13321 tacctaaagt tcagtttaat ttattaaatt cttcagctta ctatcgttat ttatcaagac
    13381 gcattggcgc tattgtagaa acaacactaa gcgcattaaa atatcaaggc acgtattcaa
    13441 agtttatgcc aaaacatttt gagacaagtt ttagaaggaa accaagaaca aatgacgaat
    13501 taattgcaca aacattaacg acaactcaag gtattccaat taatattaga gggcaaattg
    13561 accgtatcga tacgtataca aagaatgata caagttttgt taatatcatt gactataaat
    13621 cctctgaagg tagtgcgaca cttgatttaa cgaaagtata ttatggtatg caaatgcaaa
    13681 tgatgacata catggatatc gttttacaaa ataaacaacg ccttggatta acagatattg
    13741 tgaaaccagg tggattatta tacttccatg tacatgaacc tagaattaaa tttaaatcat
    13801 ggtctgatat tgatgaagat aaactagaac aagatttaat taaaaagttt aagttgagtg
    13861 gtttagttaa tgcagaccaa actgttattg atgcattgga tattcgttta gaacctaaat
    13921 tcacttcaga tattgtacca gttggtttga ataaagatgg ctctttgagt aaacgaggca
    13981 gccaagtggc agatgaagca acgatttata aattcatcca acataacaaa gagaatttta
    14041 tagaaacagc ttcaaatatt atggatggac atactgaagt tgcaccatta aagtacaaac
    14101 aaaaattgcc atgtgctttt tgtagttatc aatcggtatg tcatgtagat ggcatgattg
    14161 atagtaagcg atatcgaact gtagatgaaa caataaatcc aattgaagca attcaaaata
    14221 ttaacattaa tgatgaattt gggggtgagc aatagatgac aattccagag aaaccacaag
    14281 gcgtgatttg gactgacgcg caatggcaaa gtatttacgc aactggacaa gatgtacttg
    14341 ttgcagccgc ggcaggttca ggtaaaacag ctgtactagt tgagcgtatt atccaaaaga
    14401 ttttacgtga tggcattgat gtcgatcgac ttttagtcgt aacgtttaca aacttaagcg
    14461 cacgtgaaat gaagcatcgt gtagaccaac gtattcaaga ggcatcgatt gctgatcctg
    14521 caaatgcaca cttgaaaaac caacgcatca aaattcatca agcacaaata tctacactcc
    14581 atagtttttg cttgaaatta attcaacagc attatgatgt attaaatatt gacccgaact
    14641 ttagaacaag cagtgaagct gaaaatattt tattattaga acaaacgata gatgaggtca
    14701 tagaacaaca ttacgatatc cttgatcctg cttttattga attaacagag caattgtctt
    14761 cagatagaag tgatgatcag tttcgaatga ttattaaaca attgtatttc tttagcgttg
    14821 caaatccaaa tcctaaaaat tggttggatc aattggtgac accatacgaa gaagaagcac
    14881 aacaagcgca acttattcaa ctactaacag acttatctaa agtatttatc acagctgctt
    14941 atgatgcttt aaataaggcg tatgatttgt ttagtatgat ggatagcgtc gataaacatt
    15001 tagctgttat agaagatgaa cgacgtttaa tggggcgtgt tttagaaggt ggctttattg
    15061 atatacctta tttaactggt cacgaatttg gcgcgcgttt gcctaatgta acagcgaaaa
    15121 ttaaagaagc aaatgaaatg atggtcgatg ccttagaaga tgctaaactt cagtataaaa
    15181 aatataaatc attaattgat aaagtgaaga gtgattactt ttcaagagaa gctgatgatt
    15241 tgaaagctga tatgcaacaa ttggcgccac gagtaaagta ccttgcgcgt attgtgaaag
    15301 atgttatgtc agaattcaat cgaaaaaagc gtagcaaaaa tattttggat ttttctgatt
    15361 atgaacattt tgcattacaa attttaacta atgaggatgg ttcgccttca gaaattgccg
    15421 aatcataccg tcaacacttc caagaaatat tggtcgatga gtatcaagat acgaaccgag
    15481 ttcaagagaa aatactatct tgcatcaaaa cgggtgatga acataatggt aatttattta
    15541 tggttggaga tgttaagcaa tccatttata aatttagaca agctgatcca agtttattta
    15601 ttgaaaagta tcaacgcttt actatagatg gagatggcac tggacgtcga attgatttgt
    15661 cgcaaaactt ccgttctcga aaagaagtac tgtcaacgac taactatata ttcaaacata
    15721 tgatggatga acaagtcggt gaagtaaaat atgatgaagc ggcacagttg tattatggtg
    15781 caccatatga tgaatcggac catccagtaa acttaaaagt ccttgttgaa gcggatcaag
    15841 aacatagtga tttaactggt agtgaacaag aagcgcattt tatagtagaa caagttaaag
    15901 atatcttaga acatcaaaaa gtttatgata tgaaaacagg aagctataga agtgcgacat
    15961 acaaggatat cgttattcta gaacgcagct ttggacaagc tcgcaattta caacaagcct
    16021 ttaaaaatga agatattcca ttccatgtga atagtcgtga aggttacttt gaacaaacag
    16081 aagtccgctt agtattatca tttttaagag cgatagataa tccattacaa gatatttatt
    16141 tagttgggtt aatgcgctcc gttatatatc agttcaaaga agacgaatta gctcaaatta
    16201 gaatattgag tccaaatgat gactacttct atcaatcgat tgtaaattac attaatgacg
    16261 aagcagcaga tgcaatttta gttgataaat taaaaatgtt tttatcagat attcaaagtt
    16321 accaacaata tagtaaagat catccggtgt atcagttaat tgataaattt tataatgatc
    16381 attatgttat tcaatacttt agtggactta ttggtggacg tggacgacgt gcaaatcttt
    16441 atggtttatt taataaagct atcgagtttg agaattcaag ttttagaggt ttatatcaat
    16501 ttattcgttt tatcgatgaa ttgattgaaa gaggcaaaga ttttggtgag gaaaatgtag
    16561 ttggtccaaa cgataatgtc gttagaatga tgacaattca tagtagtaaa ggtctagagt
    16621 ttccatttgt catttattct ggattgtcaa aagattttaa taaacgtgat ttgaaacaac
    16681 cagttatttt aaatcagcaa tttggtctcg gaatggatta ttttgatgtg gataaagaaa
    16741 tggcatttcc atctttagct tcggttgcat atagagctgt tgccgaaaaa gaacttgtgt
    16801 cagaagaaat gcgattagtc tatgtagcat taacaagagc gaaagaacaa ctttatttaa
    16861 ttggtagagt gaaaaatgat aaatcattac tagaactaga gcaattgtct atttctggtg
    16921 agcacattgc tgtcaatgaa cgattaactt caccaaatcc gttccatctt atttatagta
    16981 ttttatctaa acatcaatct gcgtcaattc cagatgattt aaaatttgaa aaagatatag
    17041 cacaaattga agatagtagt cgtccgaatg taaatatttc aattgtgtac tttgaagatg
    17101 tgtctacaga aaccatttta gataatgatg aatatcgttc ggttaatcaa ttagaaacta
    17161 tgcaaaatgg taatgaagat gttaaagcac aaattaaaca ccaacttgat tatcgatatc
    17221 catatgtaaa tgatactaaa aagccctcaa aacaatctgt ttctgaattg aaaagacaat
    17281 atgaaacaga agaaagtggc acaagttacg aacgagtaag gcaatatcgt atcggttttt
    17341 caacgtatga acgacctaaa tttctaagtg aacaaggtaa acgaaaagcg aatgaaattg
    17401 gtacgttaat gcatacagtg atgcaacatt taccattcaa aaaagaacgc atatctgaag
    17461 ttgagttaca tcagtatatc gatggattaa tcgataaaca tattatcgaa gcagatgcga
    17521 aaaaagatat ccgtatggat gaaataatga catttatcaa tagtgagtta tattcgatta
    17581 ttgctgaagc agagcaagtt tatcgtgaat taccgtttgt agttaaccaa gcattagttg
    17641 accaattgcc acaaggagac gaagacgtct caattattca aggtatgatt gacttaatct
    17701 ttgttaaaga tggtgtgcat tattttgtag actataaaac cgatgcattt aatcgtcgcc
    17761 gtgggatgac agatgaagaa attggtacac aattaaaaaa taaatataag atacagatga
    17821 aatattatca aaatacgctt caaacgattc ttaataaaga agttaaaggt tatttatact
    17881 tcttcaaatt tggtacattg caactatagt attttgattt tcaaaagaat aaaaaataat
    17941 ttcgattaag tgcaaagtcc ttgtagcaga atgaacacaa ctcattttca aaattgtctt
    18001 acttatttat ttgttatttg ataacgaaaa aagttataat gtgaattaag ataaagatga
    18061 ggagttgaga atgaatgaaa ttcttatcat tcaagtataa tgacaaaact tcatatggcg
    18121 ttaaagtaaa acgcgaagat gctgtatggg atttaacaca agtatttgct gactttgcag
    18181 aaggagattt ccatcctaaa acattgttag ctggtttaca acaaaatcat actttagatt
    18241 ttcaagaaca agtacgtaaa gcagttgtag cagcagaaga tagcggcaaa gctgaagact
    18301 ataaaatttc atttaatgac attgaatttt taccaccagt aacacctccg aataatgtga
    18361 ttgcttttgg tagaaattac aaagatcatg cgaacgaatt aaatcatgaa gtggaaaaat
    18421 tatatgtatt tacaaaagca gcgtcatctt taacaggaga taatgcaaca attccaaatc
    18481 ataaagatat tactgatcaa ttagattatg aaggtgaatt aggtattgtt attggtaagt
    18541 ctggtgaaaa gattccaaaa gcattagctt tagattatgt ttacggctat acaattatta
    18601 acgatatcac tgatcgcaaa gcacaaagtg aacaagatca agcattttta tcaaaaagtt
    18661 taactggcgg ttgcccaatg ggtccttata tcgttactaa agacgaacta ccattacctg
    18721 aaaatgtaaa tattgttaca aaagttaaca atgaaattag acaagatggt aacactggcg
    18781 aaatgattct taaaattgat gaattaatag aagaaatttc aaaatatgtt gcactacatc
    18841 cgggagatat tattgcaact ggtacaccag caggcgttgg tgcaggtatg caaccaccta
    18901 aatttttaca accaggtgat gaagttaaag tgactattga taatattgga acgctgacaa
    18961 cttatatcgc taaataatta tcatttaaaa agctaaccag tctttatata gattggttag
    19021 ttttttcttg cttttctaaa aaggtgttaa agataaatta tttataatgt taccattttg
    19081 agatgaaagt gaaatattga tattaagaag tagttgatta ttttacagca gattcacaat
    19141 attctaataa ggaataaaat gtcatgttct tcctctcaaa tatagaagtg tggtagaata
    19201 tatattcgtg tataatcaaa tcat
//
LOCUS       DAKTIO010000037        18970 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.037, whole genome shotgun sequence.
ACCESSION   DAKTIO010000037 DAKTIO010000000
VERSION     DAKTIO010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18970
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.037"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(380..784)
                     /locus_tag="O0293_000958"
     CDS             complement(380..784)
                     /locus_tag="O0293_000958"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCY0279852.1"
                     /translation="MNTLNLDIVTPNGSVYNRDNVELVVMQTTAGEIGVMSGHIPTVA
                     ALKTGFVKVKFHDGTEYIAVSDGFVEVRKDKVSIIVQTAETAREIDVERAKLAKARAE
                     SHLENDDDNTDIHRAERALERANNRLRVAELK"
     gene            complement(804..2216)
                     /gene="atpD"
                     /locus_tag="O0293_000959"
     CDS             complement(804..2216)
                     /gene="atpD"
                     /locus_tag="O0293_000959"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCY0279853.1"
                     /translation="MGIGRVTQVMGPVIDVRFEHNEVPKINNALVIDVPKEEGTIQLT
                     LEVALQLGDDVVRTIAMDSTDGVQRGMDVKDTGKEISVPVGDETLGRVFNVLGETIDL
                     KEEISDSVRRDPIHRQAPAFDELSTEVQILETGIKVVDLLAPYIKGGKIGLFGGAGVG
                     KTVLIQELINNIAQEHGGISVFAGVGERTREGNDLYFEMSDSGVIKKTAMVFGQMNEP
                     PGARMRVALSGLTMAEYFRDEQGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTTKGSVTSIQAVFVPADDYTDPAPATAFAHLDATTNLERKLTE
                     MGIYPAVDPLASTSRALEPSIVGQEHYEVARDVQSTLQKYRELQDIIAILGMDELSDE
                     DKQTVERARRIQFFLSQNFHVAEQFTGQKGSYVPVKTTVANFKDILDGKYDHIPEDAF
                     RLVGSMDDVIAKAKDMGVEV"
     gene            complement(2238..3104)
                     /locus_tag="O0293_000960"
     CDS             complement(2238..3104)
                     /locus_tag="O0293_000960"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCY0279854.1"
                     /translation="MASLKEIDTRIKSTKKMKQITKAMNMVSSSKLRRAEKNTKQFTP
                     YMDKMQDAITAVAGASSNTNHPMLRPRKITRSGYLVITSDKGLAGAYSANVLKKLITD
                     IEAKHQDSSEYSIVVLGQQGVDFLKNRGYDIEYSQVDVPDQPSFKSVQALANHAIDLY
                     SEEEIDELNIYYSHYVSVLENKPTSRQVLPLSQEDSSKGHGHLSSYEFEPDKESILSV
                     ILPQYVESLIYGTILDAKASEHATRMTAMKNATDNATELIDDLSLEYNRARQAEITQQ
                     ITEIVGGSAALE"
     gene            complement(3135..4643)
                     /locus_tag="O0293_000961"
     CDS             complement(3135..4643)
                     /locus_tag="O0293_000961"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCY0279855.1"
                     /translation="MAIKAEEISALLRSQIENYESEMSVTDVGTVLQIGDGIALIHGL
                     NDVMAGELVEFHNGVLGLAQNLEESNVGVVILGPYTGITEGDEVKRTGRIMEVPVGEE
                     LIGRVVNPLGQPIDGQGPINTTKTRPVEKKATGVMDRKSVDEPLQTGIKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQGTICIYVAIGQKDSTVRANVEKLRQAGAL
                     DYTIVVAASASEPSPLLYIAPYSGVTMGEEFMFNGKHVLIVYDDLTKQAAAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLNDDLGGGSITALPIIETQAGDISAYVPTNV
                     ISITDGQIFLQSDLFFSGVRPAINAGQSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     ESFAQFGSDLDEFTASKLERGKRTVEVLKQDQNKPLPVEHQVLIIYALTKGYLDDIPV
                     VDITRFEDELNHWAESNATELLNEIRETGGLPDAEKFDTAINEFKKSFSKSE"
     gene            complement(4665..5204)
                     /locus_tag="O0293_000962"
     CDS             complement(4665..5204)
                     /locus_tag="O0293_000962"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCY0279856.1"
                     /translation="MVKVANKYAKALFDVSLDTNNLETINEELTVINEAVKDKIEQLR
                     MVDSNPTQTAEQRRELINGVFTDINPYIKNMMYVLADNRHISLIADVFKAFQSLYNGH
                     YNQDFATIESTYELSQEELDKIVKLVTQQTKLSKVIVDTKINPDLIGGFRVKVGTTVL
                     DGSVRNDLVQLQRKFRRVN"
     gene            complement(5204..5725)
                     /locus_tag="O0293_000963"
     CDS             complement(5204..5725)
                     /locus_tag="O0293_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCY0279857.1"
                     /translation="MTETANLFVLGAAGGVEWGTVIVQVLTFIVLLALLKKFAWGPLK
                     DVMDKRERDINRDIDDAEQAKLNAQKLEEENKQKLKETQEEVQKILEDAKVQARQQQE
                     QIIHEANVRANGMIETAQSEINSQKERAIADINNQVSELSVLIASKVLRKEISEQDQK
                     ALVDKYLKEAGDK"
     gene            complement(5923..6135)
                     /gene="atpE"
                     /locus_tag="O0293_000964"
     CDS             complement(5923..6135)
                     /gene="atpE"
                     /locus_tag="O0293_000964"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCY0279858.1"
                     /translation="MNLIAAAIAIGLSALGAGIGNGLIVSRTVEGVARQPEARGQLMG
                     IMFIGVGLVEALPIIGVVIAFMTFAG"
     gene            complement(6178..6906)
                     /gene="atpB"
                     /locus_tag="O0293_000965"
     CDS             complement(6178..6906)
                     /gene="atpB"
                     /locus_tag="O0293_000965"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCY0279859.1"
                     /translation="MDHKSPLVSWNLFGFDIVFNLSSILMILVTAFLVFLLAIICTRN
                     LKKRPTGKQNFVEWIFDFVRGIIEGNMAWKKGGQFHFLAVTLILYIFIANMLGLPFSI
                     VTKDHTLWWKSPTADATVTLTLSTTIILLTHFYGIKMRGTKQYLKGYVQPFWPLAIIN
                     VFEEFTSTLTLGLRLYGNIFAGEILLTLLAGLFFNEPAWGWIISIPGLIVWQAFSIFV
                     GTIQAYIFIMLSMVYMSHKVADEH"
     gene            complement(6927..7379)
                     /locus_tag="O0293_000966"
     CDS             complement(6927..7379)
                     /locus_tag="O0293_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HCY0279860.1"
                     /translation="MNRIDTTNRLYYHLVCMSGTVFMIDISHTEAYKMSRFYNIFKQF
                     IQYYFYLIIILGGLYLYTHHAFILGLIIGVTGSAINTCIFECYLAKAKRPDTMHISTG
                     NMWRYLVAIIACMIWYLNKAHVSIIGIIIGLMISYVVVIIRPLLKVSK"
     gene            complement(7447..8574)
                     /gene="wecB"
                     /locus_tag="O0293_000967"
     CDS             complement(7447..8574)
                     /gene="wecB"
                     /locus_tag="O0293_000967"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCY0279861.1"
                     /translation="MKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREML
                     DSVLSTFEIKPKYDLNIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFA
                     GGLAAFYNQVPIGHVEAGLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLSE
                     GKYSESVVVTGNTAIDAMKYTVDDNYKSNIMDKYHDKKFILMTAHRRENIGKPMENIF
                     KAVRRLIDEYTDLALVYPMHKNPKVREVAQKILGSHDRIELIEPLDVVDFHNFAKKSY
                     FILTDSGGIQEEAPSFNKPVLVLRSVTERPEGVEAGTLKVIGTNKQNVYQAAKELIDD
                     ERLYHQMSEASNPYGDGFASERIVNHIKYYLNLITEKPSDF"
     gene            complement(8598..9227)
                     /gene="upp"
                     /locus_tag="O0293_000968"
     CDS             complement(8598..9227)
                     /gene="upp"
                     /locus_tag="O0293_000968"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCY0279862.1"
                     /translation="MSKVHVFDHPLIQHKLSYIRDVNTGTKEFRELVDEVGMLMAYEV
                     TRDLELQDVDIETPVTKMTAKRLAGKKLAIVPILRAGLGMTDGILSLVPAARVGHIGL
                     YRDPETLKAVEYFAKLPQDITERQIIVVDPMLATGASAIEAITSLKKRGAKNIRFMCL
                     IAAPEGVEKMHEAHPDVDIYIAALDEKLNDKAYITPGLGDAGDRLFGTK"
     gene            complement(9255..10493)
                     /locus_tag="O0293_000969"
     CDS             complement(9255..10493)
                     /locus_tag="O0293_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCY0279863.1"
                     /translation="MSYITKQDKVIAEAIEREFQRQNSNIELIASENFVSEAVMEAQG
                     SVLTNKYAEGYPGRRYYGGCEFVDVTESIAIDRAKALFGAEHVNVQPHSGSQANMAVY
                     LVALGMGDTVLGMNLSHGGHLTHGAPVNFSGKFYNFVEYGVDKDTERINYDEVRKLAL
                     EHKPKLIVAGASAYSRTIDFKKFKEIADEVNAKLMVDMAHIAGLVAAGLHPNPVEYAD
                     FVTTTTHKTLRGPRGGMILCKEEYKKDIDKTIFPGIQGGPLEHVIAAKAVAFGEALEN
                     NFKTYQQQVVKNAKVLAEALINEGFRIVSGGTDNHLVAVDVKGSIGLTGKEAEETLDS
                     VGITCNKNTIPFDQEKPFVTSGIRLGTPAATTRGFDEKAFEEVAKIISLALKNSKDEE
                     KLQQAKERVAKLTAEYPLYQ"
     gene            complement(10520..11044)
                     /locus_tag="O0293_000970"
     CDS             complement(10520..11044)
                     /locus_tag="O0293_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HCY0279864.1"
                     /translation="MKDLTMLLDELKDMSFFNKGDICLIGCSTSEVIGEKIGTVGSME
                     VAETIFNALDVVSKETGVTFAFQGCEHINRAITIEKSQYNPLTMEEVSVVPDVHAGGS
                     LATYAFQHMKDPIVVEHITVPCGIDIGQTLIGMHIKHVCVPVRTSVKQVGQAIVTIAT
                     SRPKKIGGERAKYQ"
     gene            complement(11151..11570)
                     /locus_tag="O0293_000971"
     CDS             complement(11151..11570)
                     /locus_tag="O0293_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HCY0279865.1"
                     /translation="MKILFVCTGNTCRSPLAESIAKEVMPNHQFESRGIFAVNNQGVS
                     NYVEDLVEEHHLAETTLSQQFTEADLKADIILTMSYSHKELIEAHFGLQNHVFTLHEY
                     VKEAGEVIDPYGGTKEMYVHTYEELVSLILKLKDIIC"
     gene            complement(11567..12613)
                     /locus_tag="O0293_000972"
     CDS             complement(11567..12613)
                     /locus_tag="O0293_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCY0279866.1"
                     /translation="MDTKIWDVREYNEDLQQYPKINEIKDIVLNGGLIGLPTETVYGL
                     AANATDEEAVAKIYEAKGRPSDNPLIVHIHSKGQLKDFTYTLDPRVEKLMQAFWPGPI
                     SFILPLKLGYLCRKVSGGLSSVAVRMPSHSVGRQLLQIINEPLAAPSANLSGRPSPTT
                     FNHVYQDLNGRIDGIVQAEQSEEGLESTVLDCTSFPYKIARPGSITAAMITEILPNSI
                     AHADYNDTEQPIAPGMKYKHYSPNTPLTIITDIESKIGNDGKDWSSIAFIVPSNKVAF
                     IPSEAQFIQLCQDDNDVKQASHNLYDVLHSLDENENISAAYIYGFELNDNTEAIMNRM
                     LKAAGNHIIKGCEL"
     gene            complement(12697..13533)
                     /gene="prmC"
                     /locus_tag="O0293_000973"
     CDS             complement(12697..13533)
                     /gene="prmC"
                     /locus_tag="O0293_000973"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCY0279867.1"
                     /translation="MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHM
                     HDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLH
                     FLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQI
                     QFLTGDALKPLINEGIKLNGLISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAI
                     YESIIEDLPHVMEKGSPVVFEIGYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSF
                     KW"
     gene            complement(13520..14596)
                     /gene="prfA"
                     /locus_tag="O0293_000974"
     CDS             complement(13520..14596)
                     /gene="prfA"
                     /locus_tag="O0293_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCY0279868.1"
                     /translation="MFDQLDIVEERYEQLNELLSDPDVVNDSDKLRKYSKEQADLQKT
                     VDVYRNYKAKKEELADIEEMLSETDDKEEVEMLKEESNGIKAELPNLEEELKILLIPK
                     DPNDDKDVIVEIRAAAGGDEAAIFAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKE
                     ISFSVSGNGAYSKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNE
                     DLKIDTYRSSGAGGQHVNTTDSAVRITHLPTGVIATSSEKSQIQNREKAMKVLKARLY
                     DMKVQEEQQKYASQRKSAVGTGDRSERIRTYNYPQSRVTDHRIGLTLQKLGQIMEGHL
                     EEIIDALTLSEQTDKLKELNNGEL"
     gene            complement(14597..15196)
                     /locus_tag="O0293_000975"
     CDS             complement(14597..15196)
                     /locus_tag="O0293_000975"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCY0279869.1"
                     /translation="MYETYHSGWIECITGSMFSGKSEELIRRLRRGIYAKQKVVVFKP
                     AIDDRYHKEKVVSHNGNAIEAINISKASEIMTHDLTNVDVIGIDEVQFFDDEIVSIVE
                     KLSADGHRVIVAGLDMDFRGEPFEPMPKLMAVSEQVTKLQAVCAVCGSSSSRTQRLIN
                     GKPAKIDDPIILVGANESYEPRCRAHHIVAPSDNNKEEL"
     gene            complement(15543..15797)
                     /locus_tag="O0293_000976"
     CDS             complement(15543..15797)
                     /locus_tag="O0293_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCY0279870.1"
                     /translation="MKQGIHPEYHQVIFLDTTTNFKFLSGSTKTSSEMMEWEDGKEYP
                     VIRLDISSDSHPFYTGRQKFAAADGRVERFNKKFGLKSNN"
     gene            complement(15915..17231)
                     /gene="rho"
                     /locus_tag="O0293_000977"
     CDS             complement(15915..17231)
                     /gene="rho"
                     /locus_tag="O0293_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCY0279871.1"
                     /translation="MPERERTSPQYESFHELYKNYTTKELTQKAKTLKLTNYSKLNKK
                     ELVLAIMEAQMEKDGNYYMEGILDDIQPDGYGFLRTVNYSKGEKDIYISASQIRRFEI
                     KRGDKVTGKVRKPKDNEKYYGLLQVDFVNDHNAEEVKKRPHFQALTPLYPDERIKLET
                     ETQNYSTRIMDLVTPIGLGQRGLIVAPPKAGKTSLLKEIANAISTNKPDAKLFILLVG
                     ERPEEVTDLERSVEAAEVVHSTFDEPPEHHVKVAELLLERAKRLVEIGEDVIILMDSI
                     TRLARAYNLVIPPSGRTLSGGLDPASLHKPKAFFGAARNIEAGGSLTILATALVDTGS
                     RMDDMIYEEFKGTGNMELHLDRKLSERRIFPAIDIGRSSTRKEELLISKSELDTLWQL
                     RNLFTDSTDFTERFIRKLKRSKNNEDFFKQLQKSAEESTKTGRPII"
     gene            complement(17478..18905)
                     /locus_tag="O0293_000978"
     CDS             complement(17478..18905)
                     /locus_tag="O0293_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCY0279872.1"
                     /translation="MRDYTKQYINGEWVESNSNETIEVINPATEEVIGKVAKGNKADV
                     DKAVEAADDVYLEFRHTSVKERQALLDKIVKEYENRKDDIVQAITDELGAPLSLSERV
                     HYQMGLNHFVAARDALDNYEFEERRGDDLVVKEAIGVSGLITPWNFPTNQTSLKLAAA
                     FAAGSPVVLKPSEETPFAAVILAEIFDKVGVPKGVFNLVNGDGAGVGNPLSEHPKVRM
                     MSFTGSGPTGSKIMEKAAKDFKKVSLELGGKSPYIVLDDVDIKEAAKATTGKVVNNTG
                     QVCTAGTRVLVPNKIKDAFLAELKEQFSQVRVGNPREDGTQVGPIISKKQFDQVQNYI
                     NKGIEEGAELFYGGPGKPEGLEKGYFARPTIFINVDNQMTIAQEEIFGPVMSVITYND
                     LDEAIQIANDTKYGLAGYVIGKDKETLHKVARSIEAGTVEINEAGRKPDLPFGGYKQS
                     GLGREWGDYGIEEFLEVKSIAGYFK"
BASE COUNT         5584 a         3831 c         2839 g         6716 t
ORIGIN      
        1 gtcgactgta gatagtggtg aaggaaattt tgattttagc aatctattag tgattgtaaa
       61 aaagtaaaac tattcttggg agtcggacat aaatcaatgt ctagactcta tagtgttgta
      121 tttggcagta gttgacgggg ccccaacata aagaaatact ttttctttag aaattagtat
      181 ttcttataca tgagttttac tcatgtattc ctatttttaa atacacatta gctgtgttta
      241 atgataaaga accactacat cataaatctt taatggttct ttatcaattc cttcccccct
      301 ctcatgtttt gtatctttaa atcaatataa ttcaaaaaag ggtcgaagat atgaaatcac
      361 atcttcgacc ctttatttac tattttaatt cagccacacg caaacggtta tttgctctct
      421 ctaaagctct ttcggctcta tgaatatcag tattgtcgtc atcattttcc aagtgagact
      481 ctgctcttgc tttggctaat ttagctcttt caacatcaat ttctcttgca gtttctgcag
      541 tctgaacaat gattgaaact ttatcttttc taacttcaac aaagccatcg cttacagcaa
      601 tatattcagt tccatcgtga aatttcactt ttacaaagcc tgtttttaaa gcagctacag
      661 ttggaatatg tccactcatg acacctatct caccagctgt tgtttgcata acaacgagtt
      721 caacattatc acgattgtaa acagaaccat taggagtgac aatatctagg tttaatgtat
      781 tcattatcca ttcctcctaa ttgttatact tcaacaccca tatctttagc ttttgcaata
      841 acatcatcca tgctaccaac taaacggaat gcatcttctg gaatatggtc atatttacca
      901 tctaagatat ctttaaagtt tgcaactgtt gtcttaacag gtacataaga acctttttga
      961 ccagtaaatt gttccgctac gtggaagttt tgagataaga agaattgaat tctacgtgcg
     1021 cgttcaactg tttgtttatc ttcatcagat aattcgtcca tacctaagat agcaatgata
     1081 tcttgtaatt cacggtattt ttgaagtgtt gattgtacat cacgagctac ttcataatgt
     1141 tcttgaccta caattgatgg ttccaatgct cttgatgtag acgctaatgg atccacggct
     1201 ggataaatac ccatttcagt taatttacgt tctaagtttg tagttgcatc taaatgggca
     1261 aacgctgtcg caggcgctgg gtcagtatag tcatcggcag gtacgaatac cgcttgaata
     1321 gaagtaactg atccttttgt tgtagacgta atacgttctt gtaattgtcc catttcagta
     1381 gcaagtgttg gttggtaacc tactgcagaa ggcatacgac ctaataatgc agatacctca
     1441 gaaccagctt gtgtaaatct gaaaatgtta tcgatgaata ataatacgtc ttgaccttgt
     1501 tcgtcacgga aatattcagc cattgttaaa ccagataatg caacacgcat acgtgcacca
     1561 ggtggctcat tcatttgccc gaataccatg gctgttttct taattacacc actgtcactc
     1621 atttcgaagt ataaatcgtt accttcacga gtacgttcac ctacaccggc gaatacagaa
     1681 ataccaccgt gctcttgagc gatgttgtta attaattctt ggattaatac tgttttacct
     1741 acaccggcac caccgaacaa tccgatttta ccacctttaa tataaggtgc tagtaaatct
     1801 actactttaa tacctgtttc taaaatttga acttctgttg aaagttcatc gaatgctggt
     1861 gcttgacgat ggataggatc gcggcgaaca gaatcactaa tttcttcttt aaggtcaatt
     1921 gtttcaccta gtacattaaa tacacgacct aatgtttcgt caccaacagg tacactaatt
     1981 tctttgcctg tatcttttac atccatgcct ctttggacac catcagttga atccatcgca
     2041 attgtacgaa caacgtcgtc acctaattgc agcgcaactt ctaatgttag ttgtattgta
     2101 ccttcttctt taggcacatc aataaccaag gcgttattaa ttttaggaac ttcgttatgt
     2161 tcaaatcgaa catcaattac aggacccata acttgagtta cacggccaat tcccatgcta
     2221 ttttcctcct ttaaatatta ttcaagcgct gcggaaccac caacaatttc agtaatttgt
     2281 tgcgtaattt ctgcttgtct cgctctgtta tattctaatg ataagtcatc aataagttca
     2341 gttgcattat cagtggcatt tttcatcgca gtcatacgtg ttgcatgctc acttgctttt
     2401 gcgtctaata ttgttccgta aatcaaactc tcaacatatt gaggcaagat tacacttaag
     2461 atagattctt tatctggctc aaattcataa gaagacaaat gaccatgccc cttactagaa
     2521 tcctcttgag ataatggtaa tacttgtcta gatgtaggct tgttttcaag aacgctgaca
     2581 taatgactat agtatatatt taattcatca atttcttctt cactgtataa gtctatagca
     2641 tggttagcta gtgcttgaac agatttgaaa gaaggttgat caggtacgtc tacttgagaa
     2701 tactcaatgt cataacctct attttttagg aaatcaacac cttgttgccc taaaactaca
     2761 atactgtatt cgctactatc ttgatgtttc gcttcaatat cagtaatcaa ttttttaagc
     2821 acgtttgcac tatatgcacc tgctaaacct ttatcactcg tgataactaa atatccactt
     2881 ctagtaattt tacgaggtct tagcattgga tggtttgtat tgctacttgc ccctgcaact
     2941 gcagtaattg catcttgcat tttatccata tatggtgtga attgttttgt atttttttca
     3001 gctctacgaa gttttgaact tgataccatg ttcatcgctt tcgtaatctg cttcattttt
     3061 ttggttgatt ttattcgagt atctatttct ttaagagaag ccactatctc accaccttat
     3121 actaaacttg tttattattc agatttgcta aagctttttt tgaattcgtt aattgctgtg
     3181 tcaaacttct cagcatctgg taagccacca gtttctctga tttcatttaa cagttcagta
     3241 gcatttgatt ctgcccagtg gtttaactcg tcttcaaaac gtgtgatatc tacaactgga
     3301 atatcatcta aatatccttt tgttaatgca taaataatca acacttggtg ttcgactggt
     3361 aatggtttgt tttgatcttg ttttaagact tcaacagtac gtttaccacg ttctaattta
     3421 cttgcagtaa attcatcaag gtctgaaccg aattgtgcaa atgattcaag ttctctgtat
     3481 gacgctaagt caagacgtaa cgtaccagca actttcttca ttgctttaat ttgtgcagat
     3541 ccaccaacac gagatacaga ttgtccggca ttaatcgctg gtcttacacc tgagaagaat
     3601 aaatcagatt gtaagaagat ttgtccatct gtaattgaaa taacgtttgt tggtacataa
     3661 gctgaaatat caccagcttg tgtttcgata attggtaatg cagtaattga accgccacct
     3721 aagtcatcgt ttaattttgc tgctctttct aataatctac tatgtaagta gaatacgtca
     3781 cctgggtatg cttcacgacc tggaggtcta cgtaataata atgataactc acgataagct
     3841 gctgcttgtt tagttaaatc atcataaacg attaaaacat gtttaccgtt aaacatgaat
     3901 tcttcaccca ttgttacacc tgaatatggt gcaatatata ataatggaga aggttcagaa
     3961 gctgatgctg ctacaacaat agtgtagtct aaagcgcctg cttgtcttaa cttttcaaca
     4021 tttgctctta ctgttgaatc tttttgacca atagcaacat agatacaaat cgtaccttga
     4081 tctttttggt tcaaaattgt gtcaattgca attgttgttt tacctgtttg acggtcaccg
     4141 atgattaact cacgttgacc tctaccaatt ggtactaaag catcaattgc tttgatacct
     4201 gtttgtaatg gctcatctac tgatttacga tccattacac cagtagcttt tttctctact
     4261 ggacgtgttt tagttgtgtt aatcggtcct tgtccatcaa taggttgtcc taatggatta
     4321 acaactcttc cgattagttc ttcacctact ggtacttcca tgatacgacc agtacgttta
     4381 acttcgtcac cttcagtaat acctgtgtat ggtcctaaaa taaccacacc cacgtttgac
     4441 tcttcaaggt tttgggctaa accaagtacg ccgttatgga attctactag ctcaccagcc
     4501 ataacgtcat ttaatccgtg aattaatgca ataccatcac caatttgtaa tacagtacct
     4561 acatcagtta cggacatttc tgactcataa ttttcaattt gtgagcgaag taatgcactg
     4621 atttcttcag cttttatggc catctatgtc actcctcttt ataattaatt aactcttcta
     4681 aattttcttt gtaattggac aagatcattt ctaacactac catctaatac agttgtgccg
     4741 actttaactc taaatccacc aattaaatct ggattaattt ttgtatctac aataacttta
     4801 gataacttcg tttgttgagt tactagtttg acaatcttat ctaactcttc ttgactcaat
     4861 tcatatgttg actcaattgt tgcaaaatct tgattgtagt gtccgttata taagctttgg
     4921 aacgccttga atacgtcagc tattaatgag atatgtctat tatctgctaa cacgtacatc
     4981 atatttttaa tgtatggatt aatgtcagta aatacaccat taatcaactc acgacgttgt
     5041 tctgctgttt gagttggatt actgtcaacc attctcaatt gctcaatttt atcttttact
     5101 gcttcattta taactgttaa ttcttcatta atagtctcta aattatttgt atctaatgac
     5161 acgtcaaata atgctttagc atacttgtta gctactttta ccattattta tcgcctgcct
     5221 cttttagata cttgtcaacc aatgcttttt ggtcttgttc agaaatttct tttctaagaa
     5281 ctttagaagc aattaacact gatagttcag atacttgatt attaatatct gcaatggcac
     5341 gttctttttg gctattgatt tcactttgtg ctgtttcaat cataccgttt gcacgtacgt
     5401 ttgcttcatg aataatttgt tcttgctgtt gacgtgcttg aaccttagca tcttctaaaa
     5461 tcttttgaac ttcttcttgt gtttctttaa gtttttgttt attttcttct tcaagtttct
     5521 gtgcatttaa cttagcttgt tctgcgtcat cgatatctct gttaatatct ctttcacgtt
     5581 tatccattac atctttcaat ggaccccatg cgaacttttt aagtaacgca agtaacacga
     5641 tgaaagttag gacctgtaca atcacagtac cccactcaac gcctccagct gcaccaagaa
     5701 cgaataagtt agctgtttca gtcacgggtt catgacactc ctttcattaa aataatcgct
     5761 agtgttatat agaaaaaaac gacgaggtta ggacctactg aatttatgcg ataggcatgg
     5821 tgcgttcata tctcaactat ttaataaagt tcaaagatat tactatatat gcatcatgaa
     5881 ttatgtccta tgcgctttgt cccgacctct tttatctgtt aattatccag caaatgtcat
     5941 gaatgcaatt actacaccga tgataggtaa tgcctcaact aaacctacac caatgaacat
     6001 gatacccatt aattgaccac gtgcttctgg ttgacgtgct acaccttcaa ctgttcttga
     6061 aacgattaaa ccgttaccga tacctgctcc taatgctgat aaaccaattg cgattgctgc
     6121 tgcgattaaa ttcataattt aatttcctcc tgtgattgta tataattatt gaaattttta
     6181 gtgttcatct gccactttat gtgacatata aaccatcgaa agcataataa agatatatgc
     6241 ttggattgtt cctacaaata ttgaaaatgc ttgccaaaca attaatcctg ggatactaat
     6301 aatccaaccc catgctggtt cgttaaagaa taagccagca agtaatgtta atagtatctc
     6361 acctgcaaat atgttaccgt acaaacgcag accaagcgtt aatgttgaag tgaactcttc
     6421 aaaaacatta ataattgcca atggccaaaa cggctgtaca taacctttaa gatattgttt
     6481 cgtaccacgc attttaattc cataaaagtg agttaacagt attatcgttg tagacaacgt
     6541 taaagtcact gttgcatcag ctgtcggtga tttccaccac aatgtgtgat ctttcgttac
     6601 tatagaaaac ggaagaccta acatattagc tataaaaatg tacagaatca gcgttactgc
     6661 taagaagtgg aattgaccac cttttttcca agccatgtta ccttcaatga ttcccctcac
     6721 gaaatcaaaa atccattcaa cgaaattttg tttgccagtt ggtctttttt tcaaattacg
     6781 cgtacaaatg atagcaagta gaaaaacaag aaacgccgta acaagtatca tcaatatact
     6841 tgataaattg aaaacgatat caaaaccgaa taaattccaa ctcacgagcg gggatttgtg
     6901 atccataatc tcacctcttt cttaatttat ttgctcacct ttagtaaagg acgtatgata
     6961 actacaacat atgaaatcat taaaccaata attataccga tgatacttac atgcgcttta
     7021 ttaaggtacc aaatcataca ggcaataatt gcaactaagt atcgccacat atttccagtt
     7081 gaaatatgca tagtgtctgg tcttttagct ttagctaaat agcattcaaa aatacatgtg
     7141 ttgattgcag aaccagtaac accaatgatt aatcctaaga taaatgcatg gtgtgtataa
     7201 aggtataatc ctcccaatat tattattaga taaaaataat attgaatgaa ctgtttaaaa
     7261 atgttgtaga aacgactcat tttatatgcc tccgtgtgac tgatatcaat catgaaaacc
     7321 gttccactca tacaaactaa atgataatat aggcgatttg tggtgtcaat tctattcaaa
     7381 ttgttgtaaa aaaataagca tagttagaca caatttagac acaattagac aaaaagtcta
     7441 ttttcctcag aaatcgcttg gtttttcagt gattaaattc aaataatact taatatgatt
     7501 aactattctt tctgaagcaa atccatcacc gtaaggatta gatgcttcag acatttgatg
     7561 atataatctc tcatcatcga ttaattcttt tgcagcttga tacacatttt gcttatttgt
     7621 tccaatgact tttaatgtgc cagcttcaac accttcagga cgttctgtaa cacttcgcaa
     7681 aactaaaact ggcttattaa atgatggcgc ttcttcctga attccacctg aatctgtcaa
     7741 aataaaataa gattttttag caaaattatg gaaatctact acgtccaaag gttcaatcaa
     7801 ttcaattctg tcatgactac ctaaaatctt ttgagccacc tctcgaactt tcgggttttt
     7861 atgcattgga tataccagtg ctaaatcagt atactcatct attaagcgtc taaccgcttt
     7921 aaatatattt tccatgggtt tcccgatatt ttctcgtcgg tgtgctgtca taagaatgaa
     7981 ttttttgtca tggtatttat ccatgatgtt agatttataa ttgtcatcaa ctgtatattt
     8041 catagcatca atcgcagtat taccagtgac aacaacactt tctgaatatt tcccttcact
     8101 taacaaatgc gatgcagcat ttttagtagg tgcaaaatgt aagtcagcta atacaccaac
     8161 taattgtcta ttcacctctt ctggaaaagg tgaatattta tcataacttc taagccctgc
     8221 ttcaacgtgt ccaatcggca cttggttata aaatgccgct aaaccacctg caaatgtcgt
     8281 catcgtatca ccatgtacaa gtaccatgtc tggtttttct aattgaatca cttgttctaa
     8341 ttgagtgatt gatttagaag ttatctcaga aagtgtctgt cctgatttca taatattcaa
     8401 atcgtatttt ggtttgattt caaaggtact taatactgaa tcaagcattt ctctatgctg
     8461 tgctgtaaca acaacaattg gctcgagcat tttttcttgt tccaaagctt taactaaagg
     8521 agccatcttt atggcttcgg gtcttgttcc aaatatggtc ataatctttt tcatcaaact
     8581 acttatctcc gattcttcta tttagtacca aacaatctat ctccagcgtc gcctaaccct
     8641 ggtgtgatat atgctttgtc atttagcttt tcatcaagtg cagcaatata aatatctaca
     8701 tctggatgtg cttcatgcat cttttctacg ccttctggtg ctgcaattaa acacatgaag
     8761 cgaatatttt tagcgccacg tttcttcaat gaagtaatag cttcaattgc tgatgcgcct
     8821 gttgctaaca taggatcaac aacaatgatt tgtctttcag taatatcttg aggtaactta
     8881 gcaaaatact ctacagcctt taatgtttcg ggatctcgat ataaaccgat atgtccaact
     8941 ctggctgcag gtactaaact taaaatacca tcagtcatac ctaaaccagc tcttaaaatt
     9001 ggaacgatag ctaatttttt accagctaat cgtttagccg tcattttagt tacaggcgtt
     9061 tcaatatcaa catcctgaag ctctaagtct ctagttactt catatgccat caacatacca
     9121 acttcgtcta caagttctct aaattcttta gtacctgtat ttacatctct aatatagctt
     9181 agtttgtgtt gaattaatgg atgatcgaaa acgtgtactt tactcataaa aattactcct
     9241 atctttgtgt atgtttattg atatagagga tattcagctg ttaatttcgc aacgcgttct
     9301 ttagcttgtt gtaatttttc ttcatcttta ctatttttca atgctaaact gatgattttt
     9361 gcaacttcct caaaagcttt ttcatcaaat ccacgcgttg ttgcagcagg tgtacctaaa
     9421 cgtataccac tcgttacaaa aggtttttct tgatcgaacg gaatggtatt tttgttacat
     9481 gtgataccaa ctgaatctaa agtctcttca gcttctttac cagtaagtcc tatagaccct
     9541 tttacatcaa cagctactaa gtgattatct gtaccgccag aaacaattct aaatccttca
     9601 ttaattaatg cttctgcaag aacttttgcg tttttaacca cttgttgttg atacgttttg
     9661 aaattatttt ctaacgcttc tccaaaagca actgcttttg ctgcaataac atgctcaaga
     9721 ggtccacctt gaataccagg gaaaattgtt ttatctatgt cttttttata ttcttcctta
     9781 cataaaatca taccaccacg tggtccgcgt aatgttttgt gtgttgtagt tgttacaaaa
     9841 tcagcatatt ctactggatt tggatgtaaa cctgccgcta ctaatcctgc aatatgtgcc
     9901 atgtctacca ttaacttagc gtttacttca tctgcgattt ctttaaactt tttgaagtca
     9961 attgttcttg aatatgctga tgctcctgcc acaataagct taggcttatg ctctaacgct
    10021 aatttacgaa cttcatcata attgattcgt tctgtgtctt tatctactcc atattcaacg
    10081 aaattgtaga atttaccact aaaattaaca ggcgctccat gtgtcaagtg accaccatga
    10141 ctcaaattca tacctaaaac tgtgtcgccc attcctaatg caactaagta aacagccatg
    10201 ttcgcttgtg aacctgaatg tggttgaaca ttgacatgtt cagctccaaa caatgcttta
    10261 gcacgatcaa ttgcgatgct ttcagtaaca tctacaaact cacagccacc ataatatcgg
    10321 cgtcctggat agccttcagc atacttatta gtcaacactg aaccttgtgc ttccataacc
    10381 gcttccgata caaaattttc cgatgcgatt aactctatgt tgctattttg tctctgaaat
    10441 tctctctcga ttgcttctgc gataacttta tcttgcttgg tgatataaga cataaaatct
    10501 ccccttcttt caaaaaaact tattggtatt tagcacgttc gccaccaatc tttttcggcc
    10561 tagatgtggc aatagttaca attgcctgtc ctacttgctt tactgaggtc cttacaggta
    10621 cacatacatg cttaatatgc atgcctatta acgtttgacc aatatcaatt ccacaaggaa
    10681 cagtaatatg ttcgaccacg atcggatcct tcatatgctg aaaagcgtat gttgccaaac
    10741 tccctccagc atgtacatct ggaacgacgg aaacttcttc cattgttaat ggattatact
    10801 gagatttttc tattgttatc gctctgttga tatgttcaca tccttgaaaa gcaaaagtaa
    10861 cgcctgtctc tttactcaca acatctaatg cattaaaaat agtttctgca acttccatcg
    10921 aaccgacagt ccctattttt tcgccaatga cttccgatgt tgaacatcca attaaacata
    10981 tatctccttt attaaaaaag gacatatctt ttaattcgtc taataacatt gtcaaatctt
    11041 tcataaaagc ccacccttcc taaaaataaa aaaggaatat agcaaagtgc tacactcctc
    11101 tattataact tatttaactg ttaacatata ctaattatac agaattccta ctagcaaata
    11161 atatctttta attttaaaat taaacttaca agttcttcat aggtatgtac atacatttct
    11221 tttgttccac cgtatggatc tataacttct cctgcttctt ttacatattc atgcaatgtg
    11281 aaaacatgat tttgcaaacc aaagtgtgcc tctattaatt ctttgtgcga atacgacatc
    11341 gtcaaaataa tatctgcttt caaatctgct tcagtaaatt gttgcgataa ggtcgtttca
    11401 gctaaatgat gttcttcaac taagtcttca acataattcg aaacaccttg attgttcaca
    11461 gcgaatatac ctcttgattc aaattgatga tttggcataa cctcttttgc aatactttcc
    11521 gctaatgggc tacgacatgt gttacctgta caaacgaata aaatcttcat agttcacatc
    11581 ctttaataat gtgattacct gcagctttta acatgcgatt cataattgct tctgtattat
    11641 cattcagctc aaagccgtat atatacgccg ctgaaatatt ttcattttca tcaagtgaat
    11701 gtaacacatc ataaagatta tgacttgctt gtttaacatc attgtcatcc tgacataatt
    11761 gaatgaattg cgcttcactt ggtataaacg ccaccttatt actcggcaca ataaaagcta
    11821 tagaagacca atctttaccg tcatttccaa ttttgctctc aatatctgta ataattgtaa
    11881 gtggtgtatt gggtgagtaa tgcttatact tcatacctgg tgcaattggc tgttcagtat
    11941 cattataatc agcatgggcg atactattcg gaagtatttc tgtaatcatt gctgctgtta
    12001 tagaaccagg tcttgcaatt ttataaggaa aagatgtgca atctaaaacc gtactttcta
    12061 atccttcttc actttgttca gcttgaacaa taccatcgat acggccattc aaatcttgat
    12121 atacatgatt gaaagttgtt ggtgaaggtc taccacttaa attagcactt ggagcagcta
    12181 gaggttcatt tatgatttgt aataattgtc tacctacaga atggcttggc attctaacag
    12241 caactgatga taaacctcca gaaacttttc gacatagata gcctagcttt aacggcaata
    12301 taaacgaaat agggcccggc cagaatgcct gcattaactt ttctacgcgt ggatccaaag
    12361 tatatgtaaa atcttttaat tgacctttac tgtgtatatg aacaataagc ggattgtcag
    12421 atggacggcc tttagcttca tatattttag ctacagcttc ttcatctgtc gcatttgctg
    12481 caagtccata aactgtttca gttggtaaac ctattaaacc accgtttaaa acaatgtctt
    12541 ttatttcatt aattttagga tattgctgta aatcttcatt atattctcta acatcccaaa
    12601 ttttagtatc caacttaatc acgcctttct tatttatcat aatataaagc aaaaagctat
    12661 gcacttaact aatcatagca aaggcataac ttctaattac catttaaatg agacgattcg
    12721 atcgtggcca tttatatctt taataatgtc gatttttttg tcaggaaatt tatttaaaat
    12781 tattgattta agtgcctcac cttgattgta accaatttca aaaacaactg ggctgccttt
    12841 ttccataacg tgaggtaaat cttcaatgat tgattcataa atagcatatc catggttatc
    12901 tgcaaacaat gcctgatgtg gttcgaatct cgtaaccgtt ggagacatcg taaccatatc
    12961 tttttcatct atatatggtg gattagatat caagccgttc aacttgatac cttcattaat
    13021 taagggcttt aatgcatccc ctgttaaaaa ttgtatttgt gattgatgct tctcagcatt
    13081 attacgagcc atattcattg cttcaagtga aatatcagta gcaataacat ttaaatccgg
    13141 cttttcacat ttcaaagtaa ttgcaagtac accactaccc gttccgatat ctacgattgt
    13201 tgcatcatct tctaactgtt gtaagaaatg caacattact tcttcagttt caggtcttgg
    13261 tatcaaacaa tttgagttta catcaaacgt tctaccataa aatgaggcaa agccaactat
    13321 atactgtata ggctctccta ataacatacg ttgtaatgct aagtcgaact tcataatcat
    13381 cgctttcggc atatcatcat gcatgtggac tacaaagtcc gtacgcgtcc attgaaatac
    13441 atctaacatt aaccattcag ctcgtgtttg ttcaaaccct ttttgttgtg ttaaatgaat
    13501 tgcttcatct aacttttctt tataattcac cattattaag ttctttcaat ttatctgtct
    13561 gctctgataa agtcagtgca tctataattt cttctaaatg gccttccata atttgcccta
    13621 atttttgaag cgttagacct atacgatggt ctgttacacg gctttgtgga taattataag
    13681 ttcgaatacg ttctgaacga tcaccagtac cgactgctga tttacgttgt gacgcatact
    13741 tttgttgttc ttcttgaact ttcatatcgt ataaacgtgc ttttaacact ttcattgctt
    13801 tttcacggtt ttgaatttga gacttctcag aagatgttgc aatgacacca gttggtaaat
    13861 gggtaatacg tactgcagag tcagttgtgt ttacgtgctg accacctgca ccacttgaac
    13921 gatacgtgtc gatttttaaa tcttcatttc taatttcaat ttctacatct tcaacttctg
    13981 gtaaaactgc cactgtagct gttgaagtat gaatacgtcc acctgattct gtttcaggca
    14041 cacgttgaac gcggtgcgca ccattttcaa atttcaattt actatacgcg ccattaccag
    14101 aaactgagaa actaatttct ttgtaaccac catggtcact ttcagacgct tctactattt
    14161 cagttttgaa tccttgtgat tcagcatact ttgaatacat acgcattaaa tcaccagcaa
    14221 aaatcgcagc ctcatcacca cctgctgctg ctcttatttc tacaataacg tctttgtcat
    14281 cattaggatc tttaggaatc aataatattt taagctcttc ttcaagattt ggaagttcag
    14341 ctttaatacc attactctcc tcttttaaca tttctacttc ttctttatca tcagtctcac
    14401 ttaacatttc ttcaatatca gctaattctt cttttttagc tttatagtta cgataaacat
    14461 ctacagtttt ttgtaaatca gcttgctctt tagaatattt acgtaattta tctgaatcat
    14521 ttacaacatc tgggtcactt aacagttcat ttaactgttc gtatctttct tctacaatat
    14581 ctaattgatc aaacacttat aattcctcct tattattatc actaggtgct acgatatggt
    14641 gcgcgcgaca acgtggctca taactttcat tggcacctac taagataatc ggatcatcga
    14701 ttttagctgg tttaccattt attaatcgtt gcgttctact agatgaagaa ccacaaacag
    14761 cacaaactgc ttgaagtttc gttacttgtt cactgacagc catcaattta ggcattggtt
    14821 cgaacggttc gcccctaaaa tccatatcta atccagcaac aataacacgg tgtccatctg
    14881 ctgatagttt ttctactata cttacaattt catcgtcaaa aaattgcact tcgtctattc
    14941 ctataacatc aacattagtt aagtcgtgcg tcataatttc acttgcttta gaaatattaa
    15001 tcgcttcaat ggcattacca ttatgagaga ccactttttc tttatgatat cgatcatcaa
    15061 tcgccggttt aaatacaacg actttttgtt tagcgtatat accccttctt agacgtctta
    15121 ttagttcttc ggatttaccg ctaaacatac tacctgtaat acattctatc caaccggaat
    15181 ggtaagtttc atacattgag agttccacct ttttcaaaac ataatcgctt tattatatca
    15241 tatttcaaat attcataaat gtctttttca taattatatc gatattgtac atgaacaatt
    15301 attttataaa tttgtcttgc atatttatat acagctattc taatttgttg cttcttattc
    15361 gtgcgatgtt tctactcaat acaaacttta ataaaggggg aaatttatca ttatgtatta
    15421 acaacatggt attgagtcac ttctttgaac acagtcgtaa gtatataccc ttactacgct
    15481 tataaataaa aaagctgtct acaattccat tataggatgc agacagctta acgattacta
    15541 tattagttgt ttgatttaag accaaacttt ttgttgaaac gttccacacg tccatccgca
    15601 gcagcgaatt tttgacgtcc agtgtagaat ggatgtgaat cagatgaaat atctaaacga
    15661 ataactgggt attcttttcc atcttcccac tccatcattt ctgaagatgt ttttgtagaa
    15721 ccgcttaaga atttgaagtt tgtagtagta tctaaaaaga ttacttggtg atactcagga
    15781 tgaattcctt gtttcattat tttcagctcc tttgccctga accatctgga acagagttat
    15841 ttgtgagttt ttacccaata ctgtgtaatt ataaaggtaa ttaattcaaa acgcaagccc
    15901 ctatataatg tttattaaat tataggtcga cccgttttag tactttcttc tgcagacttt
    15961 tgtagctgct tgaagaaatc ttcattattc ttagaccttt taagtttgcg aataaatctt
    16021 tcagtaaagt cagttgagtc agtgaataga tttcttaatt gccataatgt gtctaattca
    16081 gatttactta tcaacaattc ttctttacgc gttgaacttc tgccaatatc aattgcaggg
    16141 aagatacgac gttcagacaa tttacgatct aaatgtaact ccatgttacc tgttccttta
    16201 aattcttcgt aaatcatatc gtccatacgt gaacccgtat caactaatgc agttgcaagt
    16261 attgttaaac ttccacccgc ttcaatattt ctcgctgcac cgaagaatgc ttttggtttg
    16321 tgtaaagatg caggatctaa accacctgat aatgtacgac cacttggtgg aataactaag
    16381 ttataagcgc gtgctaatct cgttatagaa tccattaaaa taatgacatc ttccccaatt
    16441 tctactaaac gctttgcacg ttcaagtaat aattcagcta ctttaacatg gtgttctggt
    16501 ggttcgtcaa acgttgaatg aacgacttca gcagcttcta ctgagcgttc taaatctgtt
    16561 acctcttcag gacgctcgcc aactaacaaa ataaatagct ttgcatctgg tttgttcgta
    16621 ctgattgcat tcgctatttc ttttaataac gatgttttac ctgctttagg tggcgccact
    16681 attaaaccac gttgacctaa accaatcggt gttactaaat ccatgatgcg cgttgaataa
    16741 ttttgtgttt ctgtctctaa tttaatacgc tcatctggat aaagtggtgt caaagcttgg
    16801 aaatgcggac gtttcttcac ttcttctgcg ttatggtcat tgacaaagtc aacttgtaat
    16861 aagccataat atttttcgtt atctttaggt tttctaactt tcccagttac tttatcccca
    16921 cgtttaattt caaaacgacg aatttggcta gcagatatat aaatatcttt ttccccttta
    16981 gaatagttca ctgttcttaa aaaaccataa ccatctggtt gtatatcatc taagatacct
    17041 tccatataat agttaccatc tttttccatt tgtgcttcca taatagctag aacaagttct
    17101 tttttattta atttactata gttcgtcaac ttaagagttt tagctttttg agtgagttcc
    17161 ttggtagtat agttcttgta caattcgtgg aatgattcat actgaggaga tgtacgttct
    17221 ctttcaggca tatagtttac acccatttca ttatttttta gttagtaagt aatagatagg
    17281 catgacaatg cataaataac tgtgctagca caatcccatt atgctaatcc ccaacctttt
    17341 aaatacatga ataactatag caaattttta ttttaattac aaaatctatt tataaaaatt
    17401 gcaaaattaa aacagtacac aaaacttaat tagtcatgtg cactgtttta ttgcgatttt
    17461 taattcaaaa atcaaattta tttaaaatat ccagctatag atttcacttc taagaactct
    17521 tcaataccat aatcgcccca ttcacgacct aaaccagatt gtttatagcc accaaatggt
    17581 aaatctggct ttctacctgc ttcgtttatt tctactgtac ctgcttcaat agaacgagct
    17641 actttatgca atgtttcttt gtccttacca ataacatatc ctgccaaacc atattttgta
    17701 tcatttgcaa tttgaatcgc ttcatctaaa tcgttataag tgataactga cattactggc
    17761 ccaaaaattt cttcttgtgc tatcgtcatt tgattatcta cattaataaa aattgtcgga
    17821 cgtgcaaagt atcctttttc aagtccttct ggtttaccag gaccaccata aaataattca
    17881 gcaccttctt caataccttt attaatataa ttttgtactt gatcaaattg ttttttacta
    17941 atgatagggc ctacttgtgt accatcttct cttggattac cgacacgcac ttggctaaat
    18001 tgttctttta attcagctaa gaatgcatct ttaattttgt taggcactaa aacacgtgta
    18061 ccagctgtac atacttgacc agtattatta acaactttgc ctgttgttgc tttagccgct
    18121 tctttaatat ctacgtcatc taggacgata tatggtgatt tgccaccaag ctctaatgat
    18181 acctttttaa aatctttagc ggctttttcc ataattttag aaccagtagg gcctgatcct
    18241 gtaaatgaca tcatgcgtac tttaggatgt tcagataaag gattcccaac accagcacca
    18301 tcaccattaa caaggttaaa tacaccttta ggaacaccga ctttatcaaa aatctcagct
    18361 aaaataacag ctgcaaatgg tgtttcttca gatggtttaa gtacaactgg actaccagcc
    18421 gcaaatgctg ctgctaattt taatgatgtt tggtttgtag ggaagttcca cggtgtaatt
    18481 aatccagata caccgattgc ttctttaaca actaaatcat ctccgcggcg ttcttcaaat
    18541 tcgtagttat ctaatgcgtc tctcgctgca acaaaatggt ttagtcccat ttgataatgg
    18601 acacgctcag ataatgataa aggagcacct aattcatccg taatagcttg tacaatatcg
    18661 tcttttctgt tttcatattc ttttacaatt ttatctaata acgcttgtct ttctttcaca
    18721 gatgtatgac ggaactctaa ataaacatcg tctgccgcct cgacggcttt atcaacatca
    18781 gctttattac ctttagcaac tttcccgatt acttcttcgg ttgctggatt tataacttct
    18841 atcgtttcat tactattact ttctacccat tcgccattaa tgtattgctt tgtgtagtct
    18901 ctcattattc tttcactcct caagaatgat ttatatatta tttatccgta atgcatacga
    18961 tataaacatt
//
LOCUS       DAKTIO010000038        18766 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.038, whole genome shotgun sequence.
ACCESSION   DAKTIO010000038 DAKTIO010000000
VERSION     DAKTIO010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18766
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.038"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            281..949
                     /gene="queC"
                     /locus_tag="O0293_000979"
     CDS             281..949
                     /gene="queC"
                     /locus_tag="O0293_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCY0279873.1"
                     /translation="MESVLNNEKAIVVFSGGQDSTTCLFYAKKHFKEVELVTFNYGQR
                     HDTEIEVAKQIAQDQGMKHHVLDMSLLSQLTPNALTQHDMEITNNEDGIPNTFVPARN
                     LLFLSFAGALAYQIGAKHIITGVCETDFSGYPDCRDSFIKSMNVTLSLAMDKDFVIHT
                     PLMWLNKAETWKLSDELEVLDYIRTKTLTCYNGIIGDGCGECPACHLRQRGLNQYLES
                     KGAL"
     gene            951..1370
                     /gene="queD"
                     /locus_tag="O0293_000980"
     CDS             951..1370
                     /gene="queD"
                     /locus_tag="O0293_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCY0279874.1"
                     /translation="MLQQIYPSTTHPYQFELNKDFNFSAAHHIPCEEAGICQNVHGHT
                     YFVNLTIVGDKLDDTGFLVNFSHLKKMIHGKFDHQLLNNLPAFKNKIPSTEIVAETIY
                     QIVKENLASLEHQPKCIQVFVRETPTSYVVFRPKEQV"
     gene            1374..2087
                     /gene="queE"
                     /locus_tag="O0293_000981"
     CDS             1374..2087
                     /gene="queE"
                     /locus_tag="O0293_000981"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCY0279875.1"
                     /translation="MAKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAF
                     TWDGSAKGDIKLMTAEEIYDELKRIGGDLFNHVTISGGNPALIKGIQELVDLFQDKGI
                     FSALETQGSKFQPWMTQIDDLTISPKPPSSTMTPDLKKLDEVITQCVPSSLNLKVVVF
                     DDKDYDFAKMIHHRYPDIPFYLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDMN
                     HVYVLPQLHTLLWSNKKGV"
     gene            2186..2770
                     /locus_tag="O0293_000982"
     CDS             2186..2770
                     /locus_tag="O0293_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279876.1"
                     /translation="MIVYILINIAIVVLITGFNLYRHQMQHLSLSAMLLSITINAFIN
                     TFIIDKYNFITLCTITMFIIWTILQFYIDKKLKPVYITDQKFIAIILTIVVSLTQRVT
                     DFSSTQSIYMSIPFLAPAIFIIGGIMLFISTFNSLDETAENNNKIKKLMIKGLIIINI
                     SFIVMMVLTPYWYLYLIVYLIFLLFLLWQKVYKF"
     gene            3050..3490
                     /locus_tag="O0293_000983"
     CDS             3050..3490
                     /locus_tag="O0293_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HCY0279877.1"
                     /translation="MNTKYFLAAGAVITTLALGACGNSNSQDQGNKTEQKTKSEDSNV
                     KTDKTKHLTGTFSSKNGETVEGKAEIKNGKLMLTNYKSSKGPDLYVYLTKNGDIKNGK
                     EIAMVDYDKEKQTFDLKNVDLSKYDEVTIYCKKAHVIFGGAKLK"
     gene            3832..4305
                     /locus_tag="O0293_000984"
     CDS             3832..4305
                     /locus_tag="O0293_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCY0279878.1"
                     /translation="MNKLLLLVTFIIRVGSGIVMLMQGYEKLTGGFTLKGLVPVIANN
                     TDSPEWYKWFFANIVAHTTSLFDIVVPLGEIAIGLGLIFGVFAYAASFFGAFVMINYI
                     LADMIFTYPLQLTFFILLLMSHSLLKQISLKEIINYFRGRKNRGEKIDDPLTDRG"
     gene            4280..4966
                     /locus_tag="O0293_000985"
     CDS             4280..4966
                     /locus_tag="O0293_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0279879.1"
                     /translation="MTHLLIVDDEQDIVDICQTYFEYEGYKVTTTTSGKEAISLLSND
                     IDIMVLDIMMPEVNGYDIVKEMKRQKLDIPFIYLTAKTQEHDTIYALTLGADDYVKKP
                     FSPRELVLRINNLLTRMKKYHHQPVEQLSFDELTLINLSKVVTVNGHEVPMRIKEFEL
                     LWYLASRENEVISKSELLEKVWGYDYYEDANTVNVHIHRIREKLEKESFTTYTITTVW
                     GLGYKFERSR"
     gene            4966..6021
                     /gene="saeS"
                     /locus_tag="O0293_000986"
     CDS             4966..6021
                     /gene="saeS"
                     /locus_tag="O0293_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SaeS"
                     /protein_id="HCY0279880.1"
                     /translation="MVLSIRSQIIIGVVSSILLTSTILAIAYILMWFNGHMTLTLTLT
                     TIITSCLTLLICSIFINPLIQKIKQFNIKTKQFANGNYASNDKTFNSPKEIYELNQSF
                     NKMASEITQQMNQIKSEQQEKTELIQNLAHDLKTPLASIISYSEGLRDGIITKDHEIK
                     ESYDILIKQANRLSTLFDDMTHIITLNTGKTYPPELIQLDQLLVSILQPYEQRIKHEN
                     RTLEVNFCNEIDAFYQYRTPLERILTNLLDNALKFSNVGSRIDINISENEDQDTIDIA
                     ISDEGIGIIPELQERIFERTFRVENSRNTKTGGSGLGLYIANELAQQNNAKISVSSDI
                     DVGTTMTVTLHKLDITS"
     gene            complement(6093..7076)
                     /gene="csbB"
                     /locus_tag="O0293_000987"
     CDS             complement(6093..7076)
                     /gene="csbB"
                     /locus_tag="O0293_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosylation protein
                     CsbB"
                     /protein_id="HCY0279881.1"
                     /translation="MKIRVVIPCFNEGEVITQTHQQLTEILSQDSSVKGYDYNMLFID
                     DGSTDTTIDEMQHLATIDRHVSFISFSRNFGKEAAMIAGYQHSTEFDAVIMIDCDLQH
                     PPEYIPKMVEGFMEGYDQVIAKRDRSGENFSRKTLSHLYYKLVNCFVEEVQFDDGVGD
                     FRLLSQRAVKSIASLEEYNRFSKGLFEWIGYNTKVFTYQNVERQKGESKWSFKKLFNY
                     GIDGLISFNSKPLRMMIYLGLFIFSISVLYIIYLFINIMISGVNIPGYFSTIAAILLL
                     GGIQLISIGVVGEYIGRIYYEVKARPKYIIQATNLSSLENDEKDTHKVYSK"
     gene            complement(7208..8047)
                     /locus_tag="O0293_000988"
     CDS             complement(7208..8047)
                     /locus_tag="O0293_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCY0279882.1"
                     /translation="MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNAAIDAGYRAF
                     DTAYFYDNEASLGRALKDNGVDREDLFITTKLWNDYQGYEKTFEYFNKSIENLQTDYL
                     DLFLIHWPCEADGLFLETYKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVN
                     QIEVHPYFNQQELQEFCDRHDIKVTAWMPLMRNRGLLDDPVIVKIAEKYHKTPAQVVL
                     RWHLAHNRIIIPKSQTPKRIQENIDILDFNLELTEVAEIDALNRNARQGKNPDDVKIG
                     DLK"
     gene            complement(8269..9618)
                     /locus_tag="O0293_000989"
     CDS             complement(8269..9618)
                     /locus_tag="O0293_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCY0279883.1"
                     /translation="METSTIISLIIFILLIALTTVFVGSEFALVKIRATRIEQLADEG
                     NKPAKIVKKMIANLDYYLSACQLGITVTSLGLGWLGEPTFEKLLHPIFEAINLPTALT
                     TTISFAVSFIIVTYLHVVLGELAPKSIAIQHTEKLALVYARPLFYFGNIMKPLIWLMN
                     GSARVIIRMFGVNPDAQTDAMSEEEIKIIINNSYNGGEINQTELAYMQNIFSFDERHA
                     KDIMVPRTQMITLNEPFNVDELLETIKEHQFTRYPITDDGDKDHIKGFINVKEFLTEY
                     ASGKTIKIANYIHELPMISETTRISDALIRMQREHVHMSLIIDEYGGTAGILTMEDIL
                     EEIVGEIRDEFDDDEVNDIVKIDNKTFQVNGRVLLDDLTEEFGIEFDDSEDIDTIGGW
                     LQSRNTNLQKDDYVDTTYDRWVVSEIDNHQIIWVILNYEFNEARPTIGQSDEDEKSE"
     gene            complement(9839..11020)
                     /gene="nagA"
                     /locus_tag="O0293_000990"
     CDS             complement(9839..11020)
                     /gene="nagA"
                     /locus_tag="O0293_000990"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCY0279884.1"
                     /translation="MSELIIYNGKVYTEDGKIDNGYIHVKDGQIVAIGEVDDKAAIDN
                     DTTNKIQVIDAKGHHVLPGFIDIHIHGGYGQDAMDGSYDGLKYLSENLLSEGTTSYLA
                     TTMTQSTDKIDNALTNIAKYEAEQDVHNAAEIVGIHLEGPFISENKVGAQHPQYVVRP
                     FIDKIKHFQETANGLIKIMTFAPEVEGAKEALETYKDDIIFSIGHTVATYEEAVEAVE
                     RGAKHVTHLYNAATPFQHREPGVFGAAWLNDALHTEMIVDGTHSHPASVAIAYRMKGN
                     ERFYLITDAMRAKGMPEGEYDLGGQKVTVQSQQARLANGALAGSILKMNHGLRNLISF
                     TGDTLDHLWRVTSLNQAIALGIDDRKGSIKVNKDADLVILDDDMNVKSTIKQGKVHTF
                     S"
     gene            complement(11328..13286)
                     /locus_tag="O0293_000991"
     CDS             complement(11328..13286)
                     /locus_tag="O0293_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCY0279885.1"
                     /translation="MRVTELLTKDTIAMDLMANDKNGVIDELVNQLDKAGKLSDVASF
                     KEAIHNRESQSTTGIGEGIAIPHAKVAAVKSPAIAFGKSKAGVDYQSLDMQPAHLFFM
                     IAAPEGGAQTHLDALAKLSGILMDENVREKLLHASSPEEVLAIIDEADDEVTKEEEAE
                     AEAQQVATAEQSSKQSNEPYVLAVTACPTGIAHTYMARDALKKQADKMGIKIKVETNG
                     SSGIKNHLTEQDIENATGIIVAADVHVETDRFDGKNVVEVPVADGIKRPEELINKALD
                     TSRKPFVARDGQRKGNSNDSQEKLSPGKAFYKHLMNGVSNMLPLVISGGILMAIVFLF
                     GANSFNPKSSEYNAFAEQLWNIGSKSAFALIIPILSGFIARSIADKPGFASGLVGGML
                     AISGGSGFIGGIIAGFLAGYLTQGVKAMTRKLPQALEGLKPTLIYPLLTVTATGLLMI
                     YAFNPPASWLNHLLLDGLNNLSGSNIVLLGLVIGAMMAIDMGGPFNKAAYVFATGALI
                     EGNAAPITAAMIGGMIPPLAIATAMLIFRRKFTKEQRGSIIPNYVMGMSFITEGAIPF
                     AAADPLRVIPSMMIGSGIGGAIALGLGSRITAPHGGIIVIVGTDGAHLLQTLIALLVG
                     TLVSALIYGLIKPKLTETEIEASKSMDE"
     gene            complement(13292..14212)
                     /gene="pfkB"
                     /locus_tag="O0293_000992"
     CDS             complement(13292..14212)
                     /gene="pfkB"
                     /locus_tag="O0293_000992"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCY0279886.1"
                     /translation="MIYTVTFNPSIDYVIFTNDFKIDGLNRATATYKFAGGKGINVSR
                     VLKTLDVESTALGFAGGFPGKFIIDTLNNSAIQSNFIEVDEDTRINVKLKTGQETEIN
                     APGPHITSTQFEQLLQQIKNTTSEDIVIVAGSVPSSIPSDAYAQIAQITAQTGAKLVV
                     DAEKELAESVLPYHPLFIKPNKDELEVMFNTTVNSDTDVIKYGRLLVDKGAQSVIVSL
                     GGDGAIYIDKEISIKAVNPQGKVVNTVGSGDSTVAGMVAGIASGLTIEKAFQQAVACG
                     TATAFDEDLATRDAIEKIKSQVTISVLDGE"
     gene            complement(14209..14973)
                     /locus_tag="O0293_000993"
     CDS             complement(14209..14973)
                     /locus_tag="O0293_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCY0279887.1"
                     /translation="MMIITEKRHELILEELSHKDFLTLQELIDRTGCSASTIRRDLSK
                     LQQLGKLQRVHGGAMLKENRMVEANLTEKLATNLDEKKMIAKIAANQINDNECLFIDA
                     GSSTLELIKYIQAKDIIVVTNGLTHVEALLKKGIKTIMLGGQVKENTLATIGSSAMEI
                     LRRYCFDKAFIGMNGLDIELGLTTPDEQEALVKQTAMSLANQSFVLIDHSKFNKVYFA
                     RVPLLESTTIITSEKALNQESLKEYQQKYHFIGGTL"
     gene            complement(15223..15705)
                     /gene="ybaK"
                     /locus_tag="O0293_000994"
     CDS             complement(15223..15705)
                     /gene="ybaK"
                     /locus_tag="O0293_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HCY0279888.1"
                     /translation="MAKNKKTNAMRMLDRAKIKYEVHSFEVPEEHLSGQEVAELIQAN
                     VKTVFKTLVLENTKHEHFVFVIPVSETLDMKKAAALVGEKKLQLMPLDNLKNVTGYIR
                     GGCSPVGMKTLFPTVVDKSCENYSHISVSGGLRTMQITIAVEDLITITKGKIGAVIHE
                     "
     gene            complement(15879..16331)
                     /locus_tag="O0293_000995"
     CDS             complement(15879..16331)
                     /locus_tag="O0293_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279889.1"
                     /translation="MTVKNLFLGFVAVILTVCLIGLLILATNEDALAKVHKTINTLNA
                     INVSTEDTYKKKMDILNIHTAKASEVNENVKKQNHFKHRVNANKSNSFNEQECQVIAD
                     RYADKHINDNYGLERISKTNHGYNYVYSNDNSTSKQHVSISNQGIITK"
     gene            complement(16624..17790)
                     /gene="norA"
                     /locus_tag="O0293_000996"
     CDS             complement(16624..17790)
                     /gene="norA"
                     /locus_tag="O0293_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorA"
                     /protein_id="HCY0279890.1"
                     /translation="MNKQIFVLYFNIFLIFLGIGLVIPVLPVYLKDLGLTGSDLGLLV
                     AAFALSQMIISPFGGTLADKLGKKLIICIGLILFSVSEFMFAVGHNFSVLMLSRVIGG
                     MSAGMVMPGVTGLIADISPSHQKAKNFGYMSAIINSGFILGPGIGGFMAEVSHRMPFY
                     FAGALGILAFIMSIVLIHDPKKSTTSGFQKLEPQLLTKINWKVFITPVILTLVLSFGL
                     SAFETLYSLYTADKVNYSPKDISIAITGGGIFGALFQIYFFDKFMKYFSELTFIAWSL
                     LYSVVVLILLVFANDYWSIMLISFVVFIGFDMIRPAITNYFSNIAGERQGFAGGLNST
                     FTSMGNFIGPLIAGALFDVHIEAPIYMAIGVSLAGVVIVLIEKQHRAKLKEQNM"
     gene            17999..18421
                     /locus_tag="O0293_000997"
     CDS             17999..18421
                     /locus_tag="O0293_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCY0279891.1"
                     /translation="MKLQKSNHTILLVLEKGEDIVECITTFADDQDLTFTSVSGIGAC
                     DDVVLKFFNLTTKQYEEKHITEPLELTSLLGNISRLDNGHFAHLHATFGTQSYETFSG
                     HLAKAIVSATAEIILTVTDLDIQRSFKDAVGLNLLDPQ"
     gene            18607..>18766
                     /locus_tag="O0293_000998"
     CDS             18607..>18766
                     /locus_tag="O0293_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HCY0279892.1"
                     /translation="MQSRYIARIYFLILFIVSLFETKIFQFMTLNLFLAYIPFELSLL
                     LRLFKPNKK"
BASE COUNT         6274 a         3393 c         2642 g         6457 t
ORIGIN      
        1 tgtcattatc ctgaccttga ttttaactaa tatatggtat attctattca tcgtacataa
       61 atgaatatca gaggttccta gctgaaaccc tctataaaaa actagacatt gaaatttcaa
      121 acattcgttg gtaaaccttt aatatatatt ataggagcaa cccatttctt tcgtctatct
      181 tttatggaga taggcttttt ttattttata aattaaaaac gggcttatca atgccattaa
      241 cctctttatt aatattatta atgataggag caattacaaa atggaaagtg tattaaataa
      301 tgaaaaagcc atcgttgtct ttagtggtgg tcaagacagt acaacatgtc tcttttatgc
      361 aaaaaaacat ttcaaagaag ttgaactcgt aacgtttaat tatggccaaa gacatgatac
      421 tgaaattgaa gtcgcaaaac aaattgcaca agatcaagga atgaaacatc acgttttaga
      481 tatgtcatta ttatcacaac ttactccaaa cgcattaaca caacatgata tggaaattac
      541 taataatgaa gatggtatac ctaatacatt tgttccagct agaaatttac ttttcttgtc
      601 gtttgcaggc gctctagctt atcaaattgg ggctaagcat attattacag gcgtatgtga
      661 aacagacttt tcaggctacc cagactgtcg cgatagtttt ataaaatcaa tgaacgtaac
      721 attaagccta gctatggaca aagattttgt cattcatact cctttaatgt ggttaaacaa
      781 agcagaaacg tggaaattaa gtgatgaact cgaagtttta gattatattc gtacaaaaac
      841 attaacatgc tataacggta tcattgggga tggctgtggt gaatgtccag cttgtcattt
      901 acgtcaacgt ggactaaatc aatatcttga aagtaaagga gcgctttaaa atgttacaac
      961 aaatctatcc tagtacaacg catccatatc aattcgaatt aaataaagat tttaattttt
     1021 cggctgcaca tcacattcca tgtgaagaag caggtatttg tcaaaatgtc catggtcata
     1081 cttactttgt taatttaaca attgtcggtg ataaactaga tgacactggc ttcttagtga
     1141 actttagcca tttgaaaaag atgatacacg gtaaatttga ccatcaactg ttaaataact
     1201 tacctgcttt taaaaacaaa atcccttcaa ctgaaatcgt agcggaaaca atttatcaaa
     1261 ttgttaaaga aaatttggca tcgctcgaac accaaccaaa atgtattcaa gtatttgtaa
     1321 gagaaacacc aacaagttat gtcgtattta gaccaaagga acaggtgtaa ctaatggcta
     1381 aaattcctgt actcgaaata ttcggtccaa ccattcaggg tgaaggtcga gtaatcggta
     1441 gaaaaacgat gtttgtgaga acagctggat gtgattatcg ctgtagctgg tgtgattctg
     1501 catttacttg ggatggtagt gctaaaggcg atataaaact catgaccgct gaagaaattt
     1561 atgatgaatt aaaacgaatt ggtggcgatt tatttaacca cgttacaatt tctggtggta
     1621 acccagcatt aattaaaggt atccaagagt tagttgactt atttcaagat aaaggtattt
     1681 tcagcgcact ggagacacaa ggcagtaaat tccaaccttg gatgacacaa attgatgatt
     1741 taacgattag tccaaaacca ccaagttcaa ctatgacacc tgatttaaaa aagttagatg
     1801 aagtgattac acaatgcgta ccttcatcat taaacttaaa agtagttgta ttcgacgata
     1861 aagattatga ttttgccaaa atgatacacc accgttatcc agatattccc ttctatttac
     1921 aagttggtaa cccatattta tcagacagcg tagataatca taccgaaaag ttgttagaac
     1981 gctacgagca gttggtggat ttagttatgc aaagtaatga tatgaatcac gtttatgtgt
     2041 taccacaact tcatacatta ctttggagca ataaaaaagg tgtataaagg ataacttatt
     2101 ttactttttg caagtcatat ctaatgaata acaatatatt aattgatata atagttgatt
     2161 gaagtataac gaacaggagt ccatcatgat tgtatatata ttaattaata ttgctatcgt
     2221 cgtacttata actggattca atttatatag gcatcaaatg caacatttat cattaagtgc
     2281 aatgttgtta tctattacga taaacgcctt tataaataca ttcataattg ataaatataa
     2341 ctttattact ctgtgtacta taacgatgtt tatcatttgg acgatactac agttttacat
     2401 tgataaaaaa ttaaaacctg tatacattac agaccaaaaa tttattgcta tcatattaac
     2461 gattgttgtc agtttaacgc aacgtgttac cgacttttca agtacacaat caatatatat
     2521 gtctattcca tttttagccc ctgccatttt catcattggt ggtattatgt tgtttatttc
     2581 aacatttaat agcttagatg aaactgctga aaataataac aaaataaaga aactaatgat
     2641 taaaggactt atcattatta acatttcatt tatcgttatg atggttttaa caccatattg
     2701 gtacttgtat ttaatcgtct atcttatttt cttgttgttt ttattgtggc aaaaggttta
     2761 taaattttaa taccaaaact attaaacact tctgatattc ttagttcaaa atatcagaag
     2821 tgttttatag tgttatctag ttcagataaa tatttcctta cttaaaaaaa cgccctcctc
     2881 ttattttgac ccctatttat ttaaatcaga caattatttt cattttcaaa ttattctttc
     2941 ttcaatatta gttaagcgat atttaaacga agttaagaat tagttaatgg catattattt
     3001 gccttcattt taaacttaac ttatcaaatt gaagaaatga ggagttagca tgaatacaaa
     3061 atatttttta gcagctggtg ctgttatcac aacattagct ttaggtgctt gtggtaattc
     3121 taattcacaa gatcaaggta acaaaactga acaaaaaaca aagtcagaag atagcaatgt
     3181 taaaactgat aaaacaaaac acctaacagg tacattcagt tctaaaaacg gtgaaactgt
     3241 tgaaggtaaa gctgagatta aaaatggtaa attaatgctt actaactaca aatcatcaaa
     3301 aggtccagat ttatacgtct acctaacaaa aaatggcgac attaaaaacg gtaaagaaat
     3361 cgcaatggtt gactacgata aagaaaaaca aacatttgat cttaaaaatg tagatttaag
     3421 caaatatgat gaagtaacaa tctattgtaa aaaagctcac gtcatcttcg gcggcgctaa
     3481 attaaaataa tcaatttctg agttaaactt ttatttacaa catactatta ctatacatta
     3541 caaattttta aaatatacat tacactcatt actcaatgga aagcgtatga tttcacagcc
     3601 cccctagctt gtagaaatca tactttcctt ttttcaatat atatacaact attaaatccc
     3661 ataagattgc agagcacata agtaaatttt tttagagctt gaggtttgtt tagcttaagc
     3721 aacccatgag ctcaaacact tcctgttcac ataacactac aaatcgcatt atgttgctta
     3781 atcttatgtt tatataaatt acacacaata aatagaaaga atgtgaacat catgaataaa
     3841 ttattgctac tcgttacatt tatcattcgt gtgggttcag gtattgttat gttaatgcaa
     3901 ggctacgaaa aattaacggg cggatttacg ctgaaaggtt tagtaccagt catcgctaac
     3961 aatactgatt caccagagtg gtataagtgg tttttcgcaa atatagttgc acatacgacg
     4021 tcattatttg atattgttgt cccactcgga gagattgcaa ttggattagg tttaattttt
     4081 ggagtttttg catatgctgc tagtttcttt ggagcctttg ttatgataaa ttatatctta
     4141 gcagatatga tatttacgta tcctcttcaa ttaactttct ttatcctttt actaatgagt
     4201 cactcattgt taaaacagat ttcacttaaa gaaatcatta attactttag aggtcgtaag
     4261 aacagaggtg aaaaaataga tgacccactt actgatcgtg gatgatgaac aagacattgt
     4321 agacatttgt caaacctatt ttgaatatga aggttacaaa gtaacaacga caactagcgg
     4381 taaagaagca atttctttac tatcaaatga tattgatatc atggtacttg atatcatgat
     4441 gccagaagtt aatggttacg acattgtcaa agaaatgaaa aggcaaaaat tagatatccc
     4501 ctttatctat ttaactgcca aaacacaaga acatgatacc atttacgcct taactttagg
     4561 tgcagatgac tatgtcaaaa aaccatttag tccaagggaa ctcgttttac gtattaataa
     4621 tttacttaca agaatgaaga aataccatca tcaaccagtt gaacaactgt cgtttgatga
     4681 attaacactt attaacttaa gtaaagttgt gactgtaaat ggtcacgaag tccctatgcg
     4741 tattaaggaa tttgagttat tgtggtattt agcttctaga gaaaatgaag ttatttctaa
     4801 atcagaatta cttgaaaaag tttggggata tgactattac gaagatgcta ataccgtgaa
     4861 tgtccatata caccgtatta gagaaaaatt agaaaaagag agctttacaa catataccat
     4921 cacaactgta tggggattag gatataaatt tgaaaggagc cgataatggt gttatcaatt
     4981 agaagtcaaa tcattattgg cgtcgtatcg agtatactat taacttcaac tattttagca
     5041 attgcatata ttttaatgtg gtttaacggc catatgacac taactttgac cttaacgaca
     5101 ataattacaa gctgtttaac cttattaata tgtagtattt ttattaatcc acttatacaa
     5161 aaaattaagc agtttaatat aaaaactaag caatttgcta acggaaatta cgcaagcaat
     5221 gataaaacgt ttaattcacc aaaagaaatt tatgaattaa atcaatcttt taataaaatg
     5281 gcttctgaaa ttacgcaaca aatgaatcaa attaaatccg aacaacaaga aaaaacagaa
     5341 ctgattcaaa acttagccca tgatttaaaa acacctttag caagcattat ttcatattct
     5401 gaaggactac gtgatggtat aatcactaag gatcatgaga ttaaagagtc atacgacata
     5461 ttaattaaac aagcaaacag attatcaaca ttatttgatg atatgactca tattatcact
     5521 ttaaatacag gtaaaacata tcccccagaa ttaatacaac tagaccaatt acttgtatca
     5581 atattgcaac catatgagca acgtatcaaa catgaaaacc gcacattaga agtgaatttc
     5641 tgtaacgaaa ttgatgcatt ttatcaatat cgaacgccac ttgagcgtat tttaacaaac
     5701 ttacttgata atgcgctaaa attttcaaat gttggtagtc gcattgatat taatattagt
     5761 gaaaacgaag atcaagatac tatcgacatt gctattagcg atgaaggtat tggcattata
     5821 ccagaactac aagaacgtat attcgaacgt acattcagag tagaaaactc tcgtaataca
     5881 aaaacgggtg gttctggatt aggcttatat atagctaatg aactcgcgca acaaaataac
     5941 gcaaaaatca gtgtaagcag tgatatagat gtaggaacta cgatgactgt aacattacac
     6001 aaattagaca ttacgtcata atccgattta tttataaaat aaaatgcaaa gactaaaaag
     6061 aagctcccat taatgagggc ttcttttttt gtttatttag aataaacttt atgggtatcc
     6121 ttctcatcat tttcaagact tgaaagattt gtagcttgaa taatatattt aggacgtgcc
     6181 ttaacttcat aatatatcct gccaatatat tcacctacaa caccaattga aattaactgt
     6241 atgccgccta ataataaaat agctgcaatc gttgaaaaat atcccggaat attaacacca
     6301 gatatcataa tattgatgaa taaatagata atatataaca cgcttattga aaagataaac
     6361 aagccaagat aaatcatcat tctcaaaggt ttactattaa aggaaatcaa tccatcaata
     6421 ccataattaa ataacttttt aaaggaccac ttagattccc ctttttgtct ctcaacattt
     6481 tgatacgtaa acactttagt attatagcct atccattcaa ataacccttt tgaaaatcga
     6541 ttatattctt caagtgatgc aatggattta acagctcttt ggcttaaaag tctaaaatca
     6601 ccaacaccat catcaaattg tacttcttct acaaagcaat taactaactt ataatacaaa
     6661 tggcttaatg ttttgcgact aaaattttca ccacttctat cacgctttgc aatcacttga
     6721 tcatagcctt ccataaaacc ttcaaccatt ttcggaatat attcaggtgg atgttgcaaa
     6781 tcacaatcta tcatgatgac tgcatcaaat tcagtactat gctggtaacc tgcaatcata
     6841 gctgcttctt ttccaaaatt tctactaaaa gaaataaagc tgacatgcct atctattgtg
     6901 gcaagatgtt gcatttcatc tatagtggta tccgtactac catcatctat gaaaagcata
     6961 ttataatcat agcctttcac actactatct tgtgaaagta tttcagttaa ttgttgatgt
     7021 gtttgtgtaa tgacttcccc ttcattaaaa caaggaatga cgactctaat tttcatctat
     7081 gtccatcctt tttatttaaa agattttgaa tgtacctgag tattgtttta tcaaatagta
     7141 aaaaccaact catttatttt aatgtacaca ttaaaaggca ttcataaacg taaaatttaa
     7201 catccagtta ttttaaatcc ccaattttca catcatctgg atttttacct tgtcttgcat
     7261 ttctatttaa agcatcaatt tcagctactt ctgttaattc taaattaaaa tctaaaatat
     7321 ctatattttc ttgaatgcgt ttaggtgtct gagattttgg aataataatt ctattgtgtg
     7381 ctaaatgcca acgtaataca acttgtgctg gtgttttatg atatttttca gcaattttaa
     7441 caatgacagg gtcgtctagt agtcctctat ttctcatcaa aggcatccat gcagtcactt
     7501 taatatcgtg acgatcacaa aattcttgta attcttgttg gttaaaatat ggatgtacct
     7561 caatttgatt caccattggt ttgatacttg attgagccat taatttttct agatgatgaa
     7621 cattaaaatt acatacacct attgctttta ccttaccttg ctcgtaaagt tcttccatag
     7681 ctttatatgt ttctaaaaat agaccatctg cttcacaagg ccaatgtatt agaaataaat
     7741 caagataatc agtttgtaaa ttttcaatcg atttgttgaa atattcgaat gttttctcat
     7801 aaccttgata gtcattccat aacttcgttg ttataaacaa atcttctcta tcgacgccat
     7861 tatcctttaa tgctcgtcct agtgaagcct cattatcata aaagtatgct gtatcaaacg
     7921 ctctatagcc tgcgtcaatt gcagcattta caactttagt catatcttcg tcagagattt
     7981 tataaacacc taacccaact gaaggcatcg ggtatccatt atttaatatt tgtatctcat
     8041 tcaacatctt tatatctctc caatctatgt atcttttata tctttacatt accctaaatt
     8101 tttcaacaaa ctcaattaat acgaattatc gctttcaata aaaattattc attaaatcat
     8161 taaagatatt gagttccaat actattttca cttttcatta attttagtca aaaaaataac
     8221 caaccaaaaa tgaattaaat cattcttagt tggtttatat attaatatct attctgattt
     8281 ttcatcttca tcagactgtc cgatagtagg tctcgcttca ttaaattcat agtttaatat
     8341 cacccaaata atttggtggt tatcgatttc tgaaacaacc cagcgatcat aagttgtatc
     8401 cacgtaatca tctttttgta aattggtatt acgagattgt aaccatccac ctatcgtatc
     8461 aatatcctca gagtcatcaa attctatacc gaactcttca gttaaatcat ccaatagtac
     8521 tctgccattt acttggaatg tcttattatc aattttaacg atatcattca cttcatcatc
     8581 atcaaattca tcacgaattt ctccaacgat ttcttctaaa atatcttcca tcgttaaaat
     8641 acctgccgtt ccaccatatt catctataat aagactcata tgtacatgtt cacgttgcat
     8701 tctaattaat gcatcactga tacgtgttgt ctctgaaatc attggcaact catgtatata
     8761 gtttgctatt ttaatcgttt ttccagaagc gtattcagtt aaaaattctt tgacgttaat
     8821 aaatccttta atgtggtctt tatcaccatc atcagtaatt ggataacgcg taaattgatg
     8881 ttcttttatt gtttctagta attcgtctac attaaaaggt tcatttagtg taatcatttg
     8941 agttctaggt accattatat cttttgcatg tctttcatcg aatgaaaaga tattttgcat
     9001 atatgccaat tcagtttggt tgatttctcc accattataa ctattgttaa taataatttt
     9061 gatttcttct tctgacattg catcagtttg ggcatcagga tttacaccaa acattctaat
     9121 aataacacgt gcagaaccat tcatcagcca aatcaatggt ttcataatgt taccgaaata
     9181 gaacaatggt cttgcatata ctaaagcaag cttttcagta tgttgaatag ctatagattt
     9241 aggcgctaat tcaccaagta ctacatgcaa atacgtaacg attataaatg acactgcaaa
     9301 cgaaatcgtc gtcgttaatg cagttggtaa attgattgct tcaaatattg ggtgtaatag
     9361 cttttcaaac gttggttcac caagccaacc taaccctaaa gatgttactg ttatacctaa
     9421 ctgacaagca gaaagataat aatctagatt agcaatcatc ttttttacta ttttagcagg
     9481 tttatttcct tcatctgcta gctgttcaat tcttgttgct ctaattttta ctaatgcaaa
     9541 ttctgaacca acaaatacag tggttaatgc aattaataga ataaatataa tcaaactaat
     9601 tatggtcgaa gtttccaatt aattccctat ttctagggat tcacctccat gttatatgtg
     9661 tcactcatgg gtaacacgca ttcaaattta tcactatgac ttaaattaca actacatgtt
     9721 atcgccttcc catttagaca cccccagaaa aaaatgttat tgcttctatt ttatcataat
     9781 ataaagtgct tatgttaaca gattaaatct attgcataca tttaattatg attatttatt
     9841 agctaaatgt gtgaaccttg ccttgtttta ttgtagattt tacattcata tcatcatcta
     9901 gaataacaag atctgcatcc ttatttactt taatactacc ttttctatca tcgataccta
     9961 atgcaatggc ttgatttaaa cttgttactc gccataaatg atctaatgta tcacctgtaa
    10021 atgatattaa gttacgtaac ccatgattca tttttaaaat actaccagca agcgcaccat
    10081 ttgcaagacg tgcttgttgc gattgaacag ttactttttg tccacccaaa tcatattctc
    10141 cttcaggcat accttttgca cgcattgcat cggtaattaa ataaaaacgt tcattacctt
    10201 tcatacggta agcaattgca accgatgccg gatgagaatg agtgccatca acaatcattt
    10261 cggtatgtag agcatcattc aaccatgctg ctccaaaaac acctggttct ctatgttgga
    10321 atggcgtcgc tgcattatat aaatgcgtga catgtttagc tcctcgctca acagcttcaa
    10381 ctgcttcttc gtatgttgct actgtatgac caattgaaaa aataatgtca tctttatacg
    10441 tttcaagcgc ttcttttgca ccttcaactt caggtgcaaa cgtcataatc tttattaatc
    10501 cgttagcagt ctcttgaaaa tgtttaattt tatcgataaa tgggcgtaca acgtattgcg
    10561 gatgttgagc accaacttta ttttcagata taaatggtcc ttctaaatgt atacctacaa
    10621 tttccgctgc attgtgaaca tcttgctccg cttcatattt agcaatattt gtaagtgcat
    10681 tatctatttt atcagtcgat tgcgtcattg tagtggccaa gtatgatgtc gtcccttcag
    10741 acaacaaatt ttcggataga tattttaagc catcgtatga cccatccatt gcatcttgac
    10801 cataaccacc atgaatatgt atatcaataa aacctggtaa tacatgatga cctttagcat
    10861 caatcacttg aattttattt gtcgtatcat tatcaattgc tgctttatca tccacttctc
    10921 caattgcaac aatctgtcca tctttcacat gaatgtaacc attatcgatt ttgccatctt
    10981 cagtataaac tttgccgtta tatataatta attctgacac gtataaaccc ttctttattt
    11041 ataaattttc actttaagac actcatattt tactatatct gcttcgtttg gcataacttt
    11101 ttgcaaaata ttaaaaaagt ctatacattt gtggtgtcta tttcttcatt catttatcta
    11161 aaaataaaaa cactttcata tcaattcata aaaactgaat taatagaaag tgtttttaca
    11221 gctttgtata agctaccgtt aacacgatat ataaaactaa catatattca ttgaacatac
    11281 aacttaatat gaagctcttt gctaacaatc attttacatc attaaaacta ctcgtccatt
    11341 gattttgaag cttcgatttc tgtttcagtt aactttggtt tgattaaacc gtaaattaat
    11401 gctgaaacta atgtgccaac tagaagtgca ataagagttt gaagtaagtg tgcaccatca
    11461 gtaccaacaa ttacaataat accaccatgt ggcgcagtaa ttcgtgaacc taagcctaaa
    11521 gcaattgcgc cacctatacc tgaaccaatc atcattgaag gaataacacg taatggatcg
    11581 gcagctgcaa atggaatcgc accttctgta ataaatgaca tacccatcac atagttaggg
    11641 ataattgaac cacgttgttc ttttgtaaat ttacgtctaa aaattaacat cgctgtcgca
    11701 attgctaacg gtggaatcat accaccaatc attgcagctg taattggtgc tgcattacct
    11761 tcaatcaacg cacctgttgc aaaaacatat gccgctttgt tgaatggacc gcccatatca
    11821 atcgccatca tagcgccaat aactaaacct aataatacaa tattagaacc tgataaattg
    11881 tttaatccat ctaataacaa atgatttaac caagatgctg gtggattaaa ggcataaatc
    11941 atcaataagc ctgtagccgt cactgttaat agtggataaa ttaatgttgg ctttaatccc
    12001 tctaatgctt gtggtaactt acgtgtcatg gctttaacac cttgtgttaa gtaacctgct
    12061 aagaaacctg caataatacc accaataaat cctgaaccac ctgaaattgc taacatacca
    12121 cctacaagac ctgaagcgaa accaggttta tccgcaatac tacgtgcaat gaatccagat
    12181 aaaattggaa tgattaacgc gaatgcactt ttactaccaa tgttccaaag ctgctctgca
    12241 aacgcattgt actctgagct ttttggatta aatgaatttg ctccaaataa aaatacaatt
    12301 gccattaaaa taccaccaga tattacaagt ggcaacatgt tagaaacacc gttcattaag
    12361 tgtttataga atgctttacc tgggcttaat ttttcttgac tgtcatttga gttacctttt
    12421 ctttgaccat cacgggcaac aaaaggttta cgacttgtat ctaatgcttt attaattaat
    12481 tcttctgggc gtttaatacc atctgctact ggtacttcta cgacattttt accatcgaag
    12541 cgatccgtct caacatgaac atcagcagca acaatgatac ctgttgcatt ttcaatatct
    12601 tgttcagtta aatggttttt aatgccgctt gaaccattcg tttctacttt aattttaata
    12661 cccattttat ccgcttgctt tttcaatgca tcacgtgcca tatatgtgtg tgcaatacct
    12721 gttggacaag cagttactgc taacacatat ggctcattag attgtttaga tgattgttct
    12781 gcagttgcaa cttgttgtgc ttcagcttct gcctcttctt cttttgtcac ttcatcatca
    12841 gcctcatcta tgatcgctag tacttcttca ggtgatgaag catgtaataa tttctcacgt
    12901 acattttcat ccattaaaat accagacaac ttagctaaag catctagatg tgtttgggcg
    12961 ccaccttctg gcgctgcaat cataaagaat aagtgtgctg gttgcatatc caaactttga
    13021 taatctacgc ctgctttaga tttaccaaac gcaatagctg gtgacttaac tgcggccact
    13081 ttggcatgtg gaatggcaat accttcgccg ataccagttg tactttgtga ttctcgattg
    13141 tgaatcgctt ccttaaatga cgcgacatca cttaatttac ctgctttgtc taattgattt
    13201 actaactcat caataacacc atttttgtca tttgccatta aatccattgc tattgtatct
    13261 tttgttaata actctgttac tctcattatt ttcactcccc atcaagtacg ctaatcgtaa
    13321 cttgtgattt tattttttct atagcgtccc gtgttgctaa gtcctcatca aatgccgtgg
    13381 cagtaccgca tgcgactgct tgttggaatg ctttttcaat cgttaaacct gaagcaattc
    13441 cagccaccat gcctgcaact gtactatcac cagagccaac tgtattaacc actttccctt
    13501 gtggattaac tgctttaata ctgatttctt tatcaatata aatagcacca tcaccgccaa
    13561 gcgagacaat aacagattgc gcacctttat caactaacaa acgaccatat ttaataacat
    13621 ctgtgtctga gttcactgtt gtattaaaca tcacttctaa ttcatcttta ttaggtttaa
    13681 taaatagtgg atgatatggt aaaacgcttt cagccaattc tttttcagcg tcgactacta
    13741 atttagcacc tgtctgtgct gtaatttgtg caatttgcgc atacgcatcg cttggaatac
    13801 tacttggtac acttccagca acaataacta tatcttcgct tgttgtattt ttaatttgtt
    13861 gtaacagttg ttcaaattgt gttgacgtta tatgaggacc cggtgcattg atttctgttt
    13921 cttgtcctgt ttttaatttc acattaatac gtgtatcttc atcaacttca ataaaattcg
    13981 attgaattgc actgttattt aatgtatcta taatgaattt cccaggaaat ccacctgcaa
    14041 atcccaaggc agttgactca acatccaatg tctttaagac gcgcgagaca ttaatacctt
    14101 tccccccagc gaatttatat gttgctgttg ctctgttcaa accatcaatt ttaaaatcat
    14161 tcgtaaaaat gacatagtca attgaaggat tgaaagtcac tgtataaatc ataaagtccc
    14221 tcctataaag tgatactttt gttggtattc ttttaacgat tcttgattta atgctttttc
    14281 agatgtgatg attgtcgtac tttctagcaa aggtacacga gcaaaatata ctttattaaa
    14341 cttagaatga tctataagta caaatgattg attggctaat gacattgctg tttgtttaac
    14401 taatgcctct tgctcatcgg gagtagttaa tccaagttca atatctaatc cattcatccc
    14461 gataaaagct ttatcgaaac aatatcgtct taatatctcc atagcactag aaccaatcgt
    14521 agcaagtgta ttttctttaa cttgaccacc tagcataatt gttttaatac cttttttaag
    14581 taaagcttct acatgtgtta aaccattagt taccacaatg atatctttcg cttgaatata
    14641 tttaattagc tccaatgtag atgaaccagc atcgataaat aagcattcat tatcgttgat
    14701 ttgattagct gctattttag caatcatttt cttttcatca agattcgttg ctaatttttc
    14761 agttaaattc gcctcaacca tacgattttc ttttaacatt gcaccaccat gcacacgttg
    14821 cagtttccct aattgttgta gtttagataa atctcttcgt attgttgaag cactgcaacc
    14881 agttcgatct attaattctt gtaaagtcaa aaaatctttg tgcgaaagtt cttctaatat
    14941 taactcgtgt cttttttcag taattatcat catcacccca tctcactttg tattataatg
    15001 agtacgcttt cacttttcaa tcataaatta ccaaaaacaa tcacaaacac tcaaattgtg
    15061 acgataaaat tacattttta tgttcaaata ctcaattata atgactgttt atgatcgtaa
    15121 aatgctaatt caatcaaaaa ggagtgtggc agaaatgaat tttataaaaa tttcatctct
    15181 gtcacactcc taatcttgcc ccatactttt tgttgttatt aatcattcat ggataactgc
    15241 tccaattttg cctttagtta ttgtaatcaa atcctcaaca gctattgtga tttgcattgt
    15301 tcgaagccca ccactcacac tgatatgact ataattttca cacgatttgt caacgactgt
    15361 tggaaacaat gttttcatac caacaggcga acacccacca cgaatgtatc ccgttacatt
    15421 tttcaaatta tctaaaggca taagctgcaa tttcttctct ccaaccaaag cagctgcctt
    15481 tttcatatct aaagtttcac ttactgggat aacaaataca aaatgttcat gttttgtatt
    15541 ttctagaaca agcgttttaa atactgtttt aacatttgct tgtatgagtt ctgcgacttc
    15601 ttgaccagat aaatgttctt ctggtacctc aaagctatga acttcgtatt taatttttgc
    15661 acgatcaagc atacgcatcg cgttcgtttt cttattttta gccatttcaa cacctcattt
    15721 atacttcttc agaactatat taacatttta aaatacatta cggattggtt gattttgaca
    15781 taacatttac atcattttat aagttgaatt caccttttta aattaaatta ttgcaaaata
    15841 aaaacagtat atccaattag aatacactgt tccatctatt atttcgttat tatgccttga
    15901 tttgaaatac ttacatgttg cttactagtt gaattatcat tggaatacac ataattatat
    15961 ccatgatttg tcttagaaat tctttctaaa ccataattat cattgatatg cttatctgca
    16021 taacgatcag caataacttg gcactcttgt tcgttaaaag aatttgattt atttgcattc
    16081 acacgatgtt taaaatgatt ttgctttttc acattttcat tcacttcaga tgctttagca
    16141 gtatgaatat tgagaatatc cattttcttt ttataagtat cttcagttga tacatttatc
    16201 gcgttaagcg tattaattgt tttatgtacc ttagcaagcg catcttcatt tgttgctaat
    16261 attaataaac caattaaaca aacggttaat attacagcaa caaagcctaa aaataaattt
    16321 tttactgtca tccccttacc cacatttcct tactcatttt ttatcatctt aacacattaa
    16381 cgttaatgaa ttaggtatgt ggattgcaat ttaaaaagaa tgtaatattt gtaacatgtt
    16441 tattactcaa aatcattttt tgttacatat aacatttaaa tataaagagt aaaacattta
    16501 gtcaatcgaa tctttttaaa tgtaaagcat cgaattttct taataaaatt cataagaaaa
    16561 acgatgctaa tcattcataa tattacgctt tactttatat caatatacac caaaatactt
    16621 atgctacata ttttgttctt tcaattttgc tctatgttgc ttttcaatta aaacaataac
    16681 aacacctgct aatgaaacac ctatagccat ataaattggt gcttcaatgt gtacatcaaa
    16741 taacgcacct gcgattaaag gacctatgaa attacccata ctagtgaatg tcgagttcaa
    16801 tccgcctgca aagccttgcc tttctccagc aatattagaa aaataatttg taatggctgg
    16861 tcgtatcata tcaaaaccta tgaagacaac aaaactgatt aacattattg accaatagtc
    16921 attagcaaaa actaataata ttaagacaac aactgaatat aataatgacc aagctataaa
    16981 tgttaactct gagaaatact tcataaattt atcgaagaaa tagatttgga aaagtgcccc
    17041 aaatataccg ccacccgtaa tagcaatcga aatatcttta ggtgaataat ttaccttgtc
    17101 agctgtgtat agtgaataca atgtttcaaa tgcagataaa ccaaacgata atacaagtgt
    17161 taaaataact ggtgtaataa acactttcca gttaattttc gttagcaatt gtggctctaa
    17221 cttttggaaa ccacttgtcg tagacttttt cggatcgtga atcaatacaa ttgacattat
    17281 aaatgctaga atacctaatg ctcctgcaaa gtaaaatggc atacgatgtg aaacttctgc
    17341 cataaatcca ccaatccctg gtcctaaaat gaatccagaa ttgataatcg ctgacatgta
    17401 gccaaagttt tttgcttttt gatggcttgg tgaaatgtca gctattaaac ctgtcacacc
    17461 aggcattacc ataccagcac tcataccacc aatcactctc gataacatca ataccgaaaa
    17521 attgtggcca actgcaaaca taaattctga cactgaaaac aaaattaatc ctatacatat
    17581 aattaatttc ttccctaatt tgtcagctag cgtaccacca aacggcgata taatcatttg
    17641 agataacgca aaagcagcaa ctagtaatcc taaatcacta ccagttaatc ccaaatcttt
    17701 taaataaaca ggcaagactg gtattactaa accgatacct aaaaaaatca agaaaatatt
    17761 aaaatataag acaaaaatct gtttattcat atgctcacct cttttttctc catgtcatgc
    17821 ttaaagctgt tattcttctt ttccacgaca gattgcaatt catgctaaat attcgtaaaa
    17881 agtttctata ttgtattaca acattgctac atttgacaat attttttctt tgtaattatc
    17941 actattttcc atttaattgt ataattaatg acatattaaa ataaagggga ttgatcatat
    18001 gaaattgcaa aaaagtaatc atactatact actagtgttg gaaaaaggag aagatatcgt
    18061 tgaatgtata acaacctttg cagatgacca agacttaaca tttacatctg tcagcggcat
    18121 tggtgcatgt gatgacgttg tattaaaatt tttcaattta acaacgaaac aatacgaaga
    18181 aaaacatatt actgagccac tggaattgac gagcttatta ggtaatattt ctcgtctaga
    18241 caatggtcat tttgcacatt tgcatgctac tttcggtact caatcatatg aaacttttag
    18301 cggacatctc gctaaagcaa ttgtttctgc aactgctgaa atcattctta ctgtaactga
    18361 tttggacatt caacgttcat tcaaagatgc agtcggttta aatttacttg atcctcaata
    18421 gcactttact ttttagttga atcaaatttt attacaagtt atcataagtg cttaatcata
    18481 aatgcattct cgtttattaa tatacttcaa cgagttaaat taaattttta attaggaatt
    18541 gaatgcgttt ataactatca catttgaatg aataactttt tcattagaaa gtagtgttta
    18601 ttattgatgc aatctcgata catcgctaga atttactttt taattttatt tatcgtctcc
    18661 ctttttgaga ctaaaatttt tcaatttatg actttaaatt tgtttttagc atatatacct
    18721 tttgagctta gtctattatt acgattattc aaaccgaata aaaagt
//
LOCUS       DAKTIO010000039        18729 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.039, whole genome shotgun sequence.
ACCESSION   DAKTIO010000039 DAKTIO010000000
VERSION     DAKTIO010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18729
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.039"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..282)
                     /locus_tag="O0293_000999"
     CDS             complement(<1..282)
                     /locus_tag="O0293_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /protein_id="HCY0279893.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHH
                     STVYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAID"
     gene            544..729
                     /locus_tag="O0293_001000"
     CDS             544..729
                     /locus_tag="O0293_001000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279894.1"
                     /translation="MNKLEKWSLVFIIFTFLSSLALLYFKYNFSSVMPVLSLSILTIL
                     ILLTSIITVVSVNDEDT"
     gene            complement(952..1530)
                     /locus_tag="O0293_001001"
     CDS             complement(952..1530)
                     /locus_tag="O0293_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCY0279895.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNG
                     TPIKTIAEQWQVSRTTIYRYLNKLEAKENKNK"
     gene            complement(1794..2174)
                     /gene="blaI"
                     /locus_tag="O0293_001002"
     CDS             complement(1794..2174)
                     /gene="blaI"
                     /locus_tag="O0293_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HCY0279896.1"
                     /translation="MTNKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            complement(2164..3921)
                     /gene="blaR1"
                     /locus_tag="O0293_001003"
     CDS             complement(2164..3921)
                     /gene="blaR1"
                     /locus_tag="O0293_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HCY0279897.1"
                     /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTML
                     AGLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFTTDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIV
                     IRKAEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFN
                     IFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTPFSN
                     KNILSHSFNGKKSLLKSRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSL
                     TDYNYKKPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYK
                     IYLALFGLDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIP
                     KNYTAAQLKQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQ
                     LSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGK
                     NAELISEKILKGMGVLNDQ"
     gene            4028..4873
                     /gene="blaZ"
                     /locus_tag="O0293_001004"
     CDS             4028..4873
                     /gene="blaZ"
                     /locus_tag="O0293_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HCY0279898.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALN
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
     gene            complement(5399..5818)
                     /locus_tag="O0293_001005"
     CDS             complement(5399..5818)
                     /locus_tag="O0293_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0279899.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLIN
                     SNLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWP
                     TLEKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
     gene            5942..6913
                     /locus_tag="O0293_001006"
     CDS             5942..6913
                     /locus_tag="O0293_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCY0279900.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKD
                     MLALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEY
                     AQVPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGG
                     VGSVAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLD
                     TVGSNVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIR
                     EDIWSKLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
     gene            complement(7085..7177)
                     /locus_tag="O0293_001007"
     CDS             complement(7085..7177)
                     /locus_tag="O0293_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCY0279901.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
     gene            complement(7334..7681)
                     /locus_tag="O0293_001008"
     CDS             complement(7334..7681)
                     /locus_tag="O0293_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0279902.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKK
                     LKIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDD
                     EIREFFSKNHEGF"
     gene            complement(7700..8317)
                     /gene="cadD"
                     /locus_tag="O0293_001009"
     CDS             complement(7700..8317)
                     /gene="cadD"
                     /locus_tag="O0293_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium resistance transporter CadD"
                     /protein_id="HCY0279903.1"
                     /translation="MIQTVVAAAVLYIATAVDLLVILLIFFARAKTRKEYRDIYVGQY
                     LGSIILILVSLFLAFVLNYVPEKWILGLLGLIPIYLGIKVAIYDDCEGEKRAKKELNE
                     KGLSKLVGTVAIVTIASCGADNIGLFVPYFVTLSVTNLLLTLFVFLILIFFLVFTAQK
                     LANIPGIGEIVEKFSRWIMAIIYIALGLFIIIENDTIQTILGFIF"
     gene            complement(9433..9627)
                     /locus_tag="O0293_001010"
     CDS             complement(9433..9627)
                     /locus_tag="O0293_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279904.1"
                     /translation="MNENLEKYIKSLPLLGLLISILLIVLFFFILDVDEDLFVVFLYC
                     LLPLIVNLSIYGFYVFVKRK"
     gene            9737..10429
                     /locus_tag="O0293_001011"
     CDS             9737..10429
                     /locus_tag="O0293_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279905.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKS
                     KNIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEAN
                     DKNAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKN
                     YVEYKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAV
                     CGTAFAAGCSVG"
     gene            complement(10597..10746)
                     /locus_tag="O0293_001012"
     CDS             complement(10597..10746)
                     /locus_tag="O0293_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279906.1"
                     /translation="MKIKTFATFTHRQLDKQVNRFLKNDIHVLEIQTHSNWLYISAIV
                     FYEDK"
     gene            complement(11293..11609)
                     /locus_tag="O0293_001013"
                     /pseudo
     CDS             complement(11293..11609)
                     /locus_tag="O0293_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
     gene            complement(11602..12585)
                     /locus_tag="O0293_001014"
     CDS             complement(11602..12585)
                     /locus_tag="O0293_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCY0279907.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILR
                     DRLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGL
                     NKPNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENRTSRSSKIERPE
                     VRKSNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQ
                     EFPEQLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLK
                     KAIHDRGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
     gene            13011..13754
                     /locus_tag="O0293_001015"
     CDS             13011..13754
                     /locus_tag="O0293_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCY0279908.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDI
                     EKIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEK
                     QESRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTST
                     NTRQNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPA
                     NLNDNDSDIEEKSEETEPKKGFWSRLFGS"
     gene            complement(13981..14166)
                     /locus_tag="O0293_001016"
     CDS             complement(13981..14166)
                     /locus_tag="O0293_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0279909.1"
                     /translation="MKNRIREYRKQKGISQETLSKELKVSRQTINAIENNKYDPTLTL
                     AFKLARLFDTTVDELFS"
     gene            complement(14171..14509)
                     /locus_tag="O0293_001017"
     CDS             complement(14171..14509)
                     /locus_tag="O0293_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279910.1"
                     /translation="MNLIPFIIILIFSCLWTAITNKYLPSETLDKKGQQDRYDERQRK
                     MFIEILAKSFIWIVYCMLFTLVLKFTGLSPSIEGSWFSQYPEIFFIIVALFLMLFNYY
                     TTNKKYTSKG"
     gene            complement(14755..15363)
                     /locus_tag="O0293_001018"
     CDS             complement(14755..15363)
                     /locus_tag="O0293_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCY0279911.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRP
                     VFQEALNFVRMGDRFVVESIDRLGRNYDEIIETVNYLKEKDVQLMITSLPMMNEVIGN
                     PLLDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQ
                     KRVIYHRVVEMLEEGKAISKIAKEVNITRQTVYRIKHDKGLS"
     gene            complement(15849..16748)
                     /locus_tag="O0293_001019"
     CDS             complement(15849..16748)
                     /locus_tag="O0293_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(C) family macrolide 2'-phosphotransferase"
                     /protein_id="HCY0279912.1"
                     /translation="MTRHNEIIKCAEKYQLHIQPQTISLNESGLDFQVAFGKDKHGVE
                     WVLRLPRRPDVYKRTKPEKQTVDFLQKNVSFEIPKWKVHAKDLIAYPKLTGKPAATID
                     PEIQNYVWEIEHKPLPENFINTLAETLVDLHNIPEENINVQHINIKTIQEIKNDFQRR
                     MNKVKETYGVSDELWNRWKQWLENDELWPRHATMIHGYLHPGHIMVDNQANVTGLIDW
                     TEATHSDPSMDFIGHHRVFDDEGLEQLITAYGKAGGEIWPRMKEHIIELNAVFPMFIA
                     EFAMESGESAYETMALKELGMKE"
     gene            complement(16847..18313)
                     /gene="msr(A)"
                     /locus_tag="O0293_001020"
     CDS             complement(16847..18313)
                     /gene="msr(A)"
                     /locus_tag="O0293_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(A)"
                     /protein_id="HCY0279913.1"
                     /translation="MEQYTIKFNQINHKLTDLRSLNIGHLYAYQFEKIALIGGNGTGK
                     TTLLNMIAQKTKPESGTVETVGEIQYFEQLNMDVENDFNTLDGSLMSELHIPMHTTDS
                     MSGGEKAKYKLANVISNYSPILLLDEPTNHLDKIGKDYLKNILKYYYGTLIIVSHDRA
                     LIDQIADTIWDIQEDGTIRVFKGNYTQYQNQYEQEQLEQQRQYEQYISEKQRLSQASK
                     AKRNQAQQMAQASSKQKNKSIAPDRLSASKQKGTVEKAAQKQAKHIEKRMEHLEEVEK
                     PQSYHEFNFPQNKIYDIHNNYPIIAQNLTLVKGSQKLLTQVRFQIPYGKNIALVGANG
                     VGKTTLLEAIYHQIEGIDCSPKVQMAYYRQLAYEDMRDVSLLQYLMDETDSSESFSRA
                     ILNNLGLNEALDRSCNVLSGGERTKLSLAVLFSTKANMLILDEPTNFLDIKTLEALEM
                     FMNKYPGIILFTSHDTRFVKHVSDKKWELTGQSLHDIT"
BASE COUNT         6471 a         2835 c         2547 g         6876 t
ORIGIN      
        1 atcaatggca cgatataaat agctccattt tccttttatt ttgatgtacg tctcatcaat
       61 acgccatttg taataagctt ttttatgctt tttcttccaa atttgatata aaattggggc
      121 atattcttga acccaacggt agaccgttga atgatgaacg tttacaccac gttcccttaa
      181 tatttcagat atatcacgat aactcaatgc atatcttaga tagtagccaa cagctacagt
      241 gataacatcc ttgttaaatt gtttatatct gaaatagttc atacagaaga ctcctttttg
      301 ttaaaattat actataaatt caactttgca acagaacctc atttactata tgggcatctc
      361 tttcattatt aatacaaagc tttgtatctc cgatattagg caaaggtgta aacgaattag
      421 gtgatactat aggattcaac attatattaa tcgtttcttt aataatgatt atcttcttat
      481 tttttataaa tcgtagtaaa aatatctcct atgattatat aaaagttcaa agaggtgaag
      541 ccaatgaata aattagaaaa atggtctttg gtatttatta tttttacatt tctctcttca
      601 ttagcacttt tatatttcaa atataatttt agttcggtta tgccagtcct aagtctttct
      661 attttaacta tactaatatt gttgacgagt attattactg tagtttcagt taatgatgaa
      721 gatacttaaa acgttcatta gtgaaacagg tattatatta aaaaagcgta cgaatttgta
      781 cgcttttttc ttgtgttatt attaaaatct cataacagga aaatattatg agattttaga
      841 cgctgtcaga acgtctcttt tacatcgagt gacattgaat caaagtcttt gtatgttaat
      901 gatatataaa attatttagt agattcatta tagtaagcta gattagttgc tctacttgtt
      961 tttattctct tttgcctcta atttattgag ataacgataa atagttgtac gtgaaacttg
     1021 ccactgctct gcaattgttt taattggcgt accattatca taaagtgtct taagtaagtt
     1081 taaatcttgt ttatttaatt tttcaggtct acctccatat cggcctctag cacgtgctgc
     1141 aattcgaccg gctgatgatc gctctaaaat taaattacgt tcaaactctg caaatgctgc
     1201 gaataaatga aataataatt gtcctgtaga acttgattta tccatcgtta tattttcttc
     1261 taaactatga aaactcacac ctcgttcgtt aagttcatta actaacgtga ttaaatcctc
     1321 catgttccgt cctagtctat ctaatctcca aacaacaatc gtatctcctg acctcgcaaa
     1381 ttcaattgct ttatctaaac caggtcgctt actttttgaa ccactcatat ggtcattaaa
     1441 tatcttttca caaccatatg tatttaatcg atcctcctgt aaattcaaat tttgtaatcc
     1501 agttgaaact cttgcataac ctattttcaa aatacagacc tcctatatac acctttagtg
     1561 taccataact tgattacgag gtcttttgtg aacatagatt ttttgttggg tttttgaaca
     1621 cataattcta tgtaatactt acttatttag acaaaaaaca agagtgtaca gaaaatggtt
     1681 attttctgga cactctcatc tttaataata tattttaaat gtcattaaca aaactaattt
     1741 aataagagtc aagtatggtt tacatatgta ttatgtttga aacatacctc taattacttt
     1801 ttactaatat catttaaaat gtctcgcaat tcttcaattt ctttgttatt taattcttca
     1861 tttttcgcaa aattcagcac taaacttttc atatcccctc catacagttt attaagaaag
     1921 gttttagcag ttttcatttt aatatcgtct tctttaatat ttgatgagta aaaataaata
     1981 ttttctgatt tgtatcgttt tataatctct tttttatata gtcttgtgat taatgttcta
     2041 atcgttttat cactaacttc tttatatttt tgaatttcaa ctacaatttc attagctgat
     2101 actgattttt tatcccatat tatattcata acatcccatt cagccataga tatttcaact
     2161 tgcttattgg tcatttaaaa cacccattcc ttttaatatt ttttcactta ttaattcagc
     2221 atttttccca gacgggtttc catctgataa atgtgtagca aaataatact tatcatgatt
     2281 tgtaattacg taacctacaa accacccatt attatacttc ccgtttacta tacctgtacc
     2341 tgttttccca tacagttcat acttttcatt tttcttaatc aataatgaag aagataattg
     2401 attctttgct tttttactaa aatggttatt ttgttccatc atatttttaa aaactattac
     2461 ttgttcaaga ttagatattt tcaaactatc ttccatccaa tagcttttat aacttcccaa
     2521 attttcatta ccataattta attgcttgag ttgagccgca gtatagttct ttggtatttg
     2581 attactaata cgttcgaagt accaattaac tgaattttgc attgctgtat ttaaatcttg
     2641 ttccttattc caagactcga aaagataatg tttatgattc catgacatac gtgaattttt
     2701 atcacttata atatgacggt caagtccaaa cagtgctaaa taaattttgt aagttgaatc
     2761 aggagaatat ctttttctac tttctttttc attataaata tagtatttgt ctttcttcat
     2821 gctgtacata acgaaagatc cactatttga accaaaattt ttactttcat ctaaaatttg
     2881 gtaatcgctt tgtaagggct ttttataatt ataatcagtt aaggattgcc ccattaaaaa
     2941 ctggctttga attatcatga taaaaaaagt aaaaatacaa ataaaaatta gaattaattt
     3001 tgactgcttt ttcagatcgg cttcttttat attaattaat ctacttttga gtaatgattt
     3061 tttaccatta aatgaatggc ttaatatatt tttgttggaa aatggggttt ttaatacgga
     3121 gtccataata gattccgcat aagttttaaa ttcatttttg ttaatattat taaggacaaa
     3181 tctatcggct tctacttcat tgtcatgaat tatctttctt tttacaatat gtactaatgg
     3241 attgtaactc ataataatag agaagatatt aaagataatt aaatgtaaag tatctctatt
     3301 tttagcatga gcatattcat gcagtattat atattttagt cttttatcaa ttacactttt
     3361 aaaatatgaa ctaggaatca aaataatata tttcccatac caaaaagtta ttggagattg
     3421 aatagcctct gcttttcgaa tcacaatatt ttttttatat tgatggttga aaagtatcgt
     3481 atctatttta tttttttcat tttcgtttag ataaagtgac tgtttcttta aatatttaag
     3541 atataataag gctttcaaaa atttaaaact taaaataata actaaaacta tccaaataac
     3601 tgtgcagata ttatcaattg aatcccaatt aaacttatgg atatctgttg tgaactcttg
     3661 aataggtttg gtggtattta tgttatggtt caaatcgtgt gacttacttt caactgtggg
     3721 cgcttgatta ttcacattat taaattttat aaaagagaat ttaattggga taaaaggaat
     3781 taatccagca agcatagtta gataccaaac tttataattt aaactataat taaaatagcg
     3841 ttttaatatg tacctaaaaa atactaacaa tagaaatata aaacaaaagc ttactacgct
     3901 cattattagt aatttagcca ttttgacacc ttctttcaaa tatttataat aaacaattga
     3961 catcaatatt acaattgtaa tattattgat ttataaaaat tacaactgta atatcggagg
     4021 gtttattttg aaaaagttaa tatttttaat tgtaattgct ttagttttaa gtgcatgtaa
     4081 ttcaaacagt tcacatgcca aagagttaaa tgatttagaa aaaaaatata atgctcatat
     4141 tggtgtttat gctttaaata ctaaaagtgg taaggaagta aaatttaatt cagataagag
     4201 atttgcctat gcttcgactt caaaagcgat aaatagtgct attttgttag aacaagtacc
     4261 ttataataag ttaaataaaa aagtacatat taacaaagat gatatagttg cttattctcc
     4321 tattttagaa aaatatgtag gaaaagatat cactttaaaa gaacttattg aggcttcaat
     4381 gacatatagt gataatacag caaacaataa aattataaaa gaaatcggtg gaatcaaaaa
     4441 agttaaacaa cgtctaaaag aactaggaga taaagtaaca aatccagtta gatatgagat
     4501 agaattaaat tactattcac caaagagcaa aaaagatact tcaacgcctg ctgctttcgg
     4561 caagacttta aataaactta tcgcaaatgg aaaattaagc aaaaaaaata aaaatttctt
     4621 acttgattta atgtttaata ataaaaacgg agacacttta attaaagatg gtgttccaaa
     4681 agactataag gttgctgata aaagtggtca agcaataaca tatgcttcta gaaatgatgt
     4741 tgcttttgtt tatcctaagg gccaatctga acctattgtt ttagtcattt ttacgaataa
     4801 agacaataaa agtgataagc caaatgataa gttgataagt gaaaccgcca agagtgtaat
     4861 gaaggaattt taatattcta aatgcataat aaatactgat aacatcttat agtttgtatt
     4921 atattttgta ttatcgttga catgtataat tttgatatca aaaactgatt ttccctttat
     4981 tattttcgag atttattttc ttaattctct ttaacaaact agaaatattg tatatacaaa
     5041 aaatcataaa taatagatga atagtttaat tataggtgtt catcaatcga aaaagcaacg
     5101 tatcttattt aaagtgcgtt gcttttttct catttataag gttaaataat tctcatatat
     5161 caagcaaagt gacaatagca ttttacaagt attttttatt gttcgattag ggtaaaccta
     5221 aataatacat aaattatatt acaatatgtt tattatagag gctaaagtat cttagaaatt
     5281 tggatagtag gtttttcggg aataaaagta tagttggctt taatatataa ttaagctaag
     5341 ctatcattta tatttactcg attataattc gcacttcaac ttttttataa cgtaaaaatt
     5401 aaaacgtagt tttatttatc tttagtaata tctttttaaa cactacagtt tcttcattag
     5461 ttaaattagc gaagtaatga ttattaagtt gataggcttt cttttctaat gttggccata
     5521 agtcttttgc tttatttgta ctgtttatta gcacagttct tctattttca ttatcagttt
     5581 gacgtgttat tagatccttt ttggacatct tatctaatat tccagatatt gttgaaggtt
     5641 ctaaatttaa agtattacca atcgctttag gtgtattcaa attactatta atcaagcaat
     5701 tcaaaacacc atattctgct ggagtaatat tatagggact taataaactt ttaaaatgtt
     5761 taaaaactct attctgagaa gtactcaaca aataatttat acaattatct aattccacaa
     5821 ctaaaacctc tcttaaatca ttagtattga ctcgcttatc atatcatgat aagttttgta
     5881 ggaaaatagt tttgcaacaa aaacatactt ctacaaaaca attgtgaaag aaggctaata
     5941 aatgttcaat gcggtagtag tgagaaaaag tgcatcaaat gttagttata aagtagaaaa
     6001 agttgatgaa tctcatttat cgaagggtga tgtattagta aaagtggtat actcttcaat
     6061 aaattataag gatatgctag ctttacaata taaaggcggt gtaattagag attatcctat
     6121 gattcctggt atagattttg caggtattgt tgaatcaagt tcgaatgata agtttaaaga
     6181 aggcgataat gtattagtta ctggttatga tgttggcgtg acacatacag gtggattttc
     6241 agaatatgca caagtgcctg gagagtggat tgttcctatt ccccaagaaa tgtctattaa
     6301 agagtctatg gtatatggca cagccggttt tacggcagca ttatcaattt ttgaactaga
     6361 aaaatatgga atgaatatca aatcacaacc tgaaatttta gtgtctgggg ctacaggtgg
     6421 tgtgggcagt gttgctatac aaattttaca taaaattgga tatgaaaata tcactgctat
     6481 cgtaagaaaa gaaaagcaaa tagaaatcgc aaaaaaatta ggtgcaaaac aagtaataat
     6541 aattgaagat gataataaac cgcttaaaaa aggtattttt gattatttct tagatacagt
     6601 ggggagtaat gtaacattat acggtttaaa acgtctaaat tatcaaggtg ttgctactgt
     6661 atgtggaaat gtagcaggaa attcacttaa cgctaatata ttaccgttta ttcttagagg
     6721 tattaaatta attggcattg attctgtata tgttgatcat aatatccgtg aagatatatg
     6781 gagtaagtta gcaaatgaat ggaatatagg aaattcactt ctatacaatg aaattggatt
     6841 tgaagaattc tataaaacga tagatcagct tttaaaagga caacatttag gaagaactat
     6901 tttaaaagta taagtgtgag tattcattaa tacatttact attaattcaa tcattttgca
     6961 tttctctact aaccagtgtt ataatttata taacgaaaat gattaacaat taatgcacca
     7021 atccccacac tattgcagta gtgtggggat tttttcgatg ctggcttata aagtcgccta
     7081 ttaattattt gttgcgttta ctaagccaat aagtataata cgcaacagca cagccactga
     7141 tgactggtgc tatgatgtga acgaaaatga ttagcaaagt aatgcacctc ctctcttcgt
     7201 caattgacgc ctgagagata ggcgactcta ttattatacc atctgtgaat atacaagcat
     7261 atctacttcc gataagtcgt cttatgttat ataactttgt tttttatagt atcaagacaa
     7321 gaagaaactc gtttcaaaaa ccctcatgat ttttagagaa aaactctctt atttcatcgt
     7381 ctttaataaa ataatatgcc atctttccat ctttataaaa atcaagtacc tcatttttat
     7441 ataaaagcct taaatgatgt gaagttgaag caacactcat tttcaatatc aaagaaatat
     7501 cacaaacaca caactcatct tctttaatca aagataatat gattttcaat ttcttctcat
     7561 cacaaatttt ttctaaaatg ttaagtaatt tcttagattt atcatcttct aaaaaactta
     7621 aagcattgtt aactttatcc tcatgtacac agatcacatc acaagtattt tcataactca
     7681 tataaaatca caccctaaat taaaatataa atcctaaaat tgtttgaata gtgtcatttt
     7741 caataataat aaataaacct aaagctatat aaataatagc cataatccaa cgactaaatt
     7801 tctcaacaat ttctccaatt cctggaatat tagctaattt ttgtgcagta aacaccaaga
     7861 agaaaattaa aattaaaaag acaaacagag taagtaataa attagtaaca cttaatgtca
     7921 caaaatacgg aacaaataaa ccaatattat cggcgccaca acttgctatc gtaacaattg
     7981 caaccgtacc aactaattta gacaatcctt tttcattcaa ttctttttta gctctctttt
     8041 ctccttcaca atcatcataa atagccactt taattccaag ataaattggt attaaaccca
     8101 ataaacctaa tatccacttt tctggaacat aatttaatac aaaagctaaa aacaaactaa
     8161 ctaatattaa aataatagac cctaaatatt gaccaacata aatatctcta tattcttttc
     8221 tagtctttgc tctagcaaaa aatattaata aaattactaa taaatcaact gctgtagcaa
     8281 tataaagaac agcagcagca acaaccgttt gaatcataat acacctcatt caaaaatatt
     8341 tttgaatgta ttataactta tatttttaaa taatcatagg tgctttattc caattgcttt
     8401 attgacgttg agcctcggaa cccttaacaa tcccaaaact tgtcgaatgg tcggcttaat
     8461 agctcacgct atgccgacat tcgtctgcaa gtttagttaa gggttcttct caacatcaat
     8521 aaattttttc ggcataaatg cgtgtctatg cgtagacatg gagacacttc agaattatgg
     8581 agtgattaca tgagtttaac tatttttctt tattcagttt taggaagttt attagcaaat
     8641 tatatctacg aaaaatcttc aaaataaata actttttcgg ttctactttg tacactttat
     8701 ctacctggta gacctaattg ttattgcttt acaggtctaa aattcgtcgt tataatctag
     8761 ttaaatcccg taagggcgca cttatacgtc ggataaatcg acatgtgcaa aaacgtaatc
     8821 gaccttcgtc gattttttta tttcaaaatt aaagaaacgg cttgagatat tccgagcagt
     8881 tataaaaatt aatttgcgtt gaatattgct actacattct tgaaaaagtg atgtagattt
     8941 atagcccata acttttttga attacatcga cgatacggcg tcgaaagaaa aataaataga
     9001 ccgtatgaaa gaaaaagcta cctcacttta tctggggtag cttttatttt ttatgccctt
     9061 attttgaatt ttaagagggc attttaaaga tttaggggtg attcatatag ttttatatct
     9121 aaaacttata tatgctttta aaacgcaaat atgagccaaa taaatatatg ctattttcaa
     9181 aaacaaaaat ttgagcaaaa acaataacta tttttagaca ctgcccattt acatgcaaat
     9241 taaaaattag cataaaattc tttaaaactt ccccacgttt ttctaaattc ctgtagggcg
     9301 tttttatcaa aaatcaagac aagaagaaac tcgttcacac tcgtttcaaa aaaacaagaa
     9361 atctaatcta tacacctacc gttttccaac cccctcaaag catacaaatt aaaaacaaaa
     9421 ttattcataa tcttactttc ttttaacaaa aacataaaaa ccataaatag acaaattaac
     9481 tatcaaaggc aataaacagt ataaaaaaac cacgaataaa tcttcatcaa catctaaaat
     9541 aaaaaagaat aaaacaatta acaaaatact aattaacaaa cctaaaagtg gcaaactttt
     9601 tatatatttt tctaaatttt cattcataaa atagactcct ttcaaaaata tgtttataaa
     9661 tatattaaaa taaatattga ttttataaag cgcttactta tacaattaaa atatcttatt
     9721 atcttggagg tatcaaatga aaattataaa attattttca ataatttcct tgttcctact
     9781 ggtatttagc gcgaattttt ctaatgctta cgcaaatgaa gaacaaaaaa gttctttact
     9841 agaaaatcaa aaagaaaaat tgaaaagcaa aaacatcgaa gtatcacaaa tgaccaagaa
     9901 agaaataagt tttctagaac aattgcaaaa taaaaatgaa gatatgcaaa aattcgaaaa
     9961 gcaaatgaaa aaagaaggat ttaaaaaaat aaagctagaa gacaaaaatt ctaaattaaa
    10021 atatctagct acagaagcaa atgataaaaa tgcatatggc tacatagcta ataacgctta
    10081 taaaaatgaa aaaacaaaag aaattgtagt ttctgaaaca atatatgata actacaacaa
    10141 aaaaatcaca aaattcattg ccgaaaaaag aagtatgact gaaaacaaag agggaaaaat
    10201 acttattaac aaaaattacg ttgaatataa ggctgaaaaa gttgataaaa acagaccggt
    10261 aacatatgca ggattcaaat ggaacggcaa agcattcgct tgtagtatgg ctggacttta
    10321 tgcatgtgct cactactgtg gagtatgggc aatcgtaaat ccaatagccg gcggtgtatg
    10381 tgaagcagtt tgcggaactg catttgcagc tggatgctct gtaggttgat tttattccaa
    10441 ttgttttatt gacgttgagc ctcggaaccc ttaacaatcc caaaacttgt cgaatggtcg
    10501 gcttaatagc tcacgctatg ccgacattcg tctgcaagtt tagttaaggg ttcttctcaa
    10561 catcaataaa ttttttcggc ataaatacga tgttccttat ttatcctcat aaaaaacaat
    10621 agctgaaata tataaccaat tactgtgtgt ttgaatttct aatacatgaa tatcattctt
    10681 taaaaaccta ttaacttgtt tatctaattg acgatgagta aaagtagcga aagttttaat
    10741 cttcatgata tctctccttt tataaaaata gcaatcctta ttacaatatt aaacactttt
    10801 attttggatt ttaagaggca ttttgaagac ttagaggcaa atcatatagg ttttgtactt
    10861 aaaaatctat acagtctctt aaaacgcaaa tatgagccaa ataaatatat gctaatttca
    10921 caaacaaaaa tttcagcaaa atcagtgact aattttttag atactgccca tttacatgca
    10981 aatttaaaaa ttagcataaa atactttaaa acttccccat gtttttctaa attcctgcag
    11041 ggcttttctt atcaaaaact tagaaatttt aatttaactc taaaatctcc gtaagagcat
    11101 aagggaaatt tgctatcatt caaaaatgat agatctggca aggccagaca ttccaagggt
    11161 tttaagttat tttaaagtga tatcatacaa agctgtagtg tcacactttt gtgacactac
    11221 atacagcatt tgtcacaaaa ataagacagc acttttttac agaaaaacaa taaaaaaaga
    11281 catcagttat cactaatgtc ttaaataata attttgcttt tcgaaattcc aaattgcggt
    11341 aattgaatgt gctttttttc ttcgtctgtc gttttatctt catcacttgt attaatcaaa
    11401 ttaccatctt cggcatcgtc taagtttaaa attttatgct tttgtttaag caatccacca
    11461 taacttaaca tacgttttcg atacaaacct ttttctaaat cacttacaat ttcttgattt
    11521 ttttcgtcgt catcagttaa aaagtcagat gatttaacgg aatattttga tgtctcttcg
    11581 agtgttataa aatcatttga attattgatt aaagatatta tattgtttca tgtttttacg
    11641 gcgcatataa agggcatgtg tttcttcaag ttcagttaga attgtttgca ttaaataagc
    11701 ttgcattgac ttgattgttt ccttttggcc tttaactccc ctatcatgta tagctttttt
    11761 aagtcttttt aaagaagtta atatttcatc ctcaatgtcc tctaacatgt aatgcatatc
    11821 ttcatgcctt gaattaaatg atttttttgc tttaagaata acagccttta tgatttctaa
    11881 atctctataa tcatatttta ataaataatt agttagttgt tctgggaatt cttgcaatag
    11941 catttcttta tcatcaaaat tatgtgtaaa ttgatttgaa tgatttgaat gattactaat
    12001 ttcatttttg tactttatat cattcaagtc attcatatca ttattctcag tatcattaaa
    12061 gtcaatgtca ttaaagtcag tatcattagt gttcgatttt cgaacttctg gacgttcgat
    12121 tttcgaactt ctggacgttc gattttcgaa cttctggacg ttcgattttc gaacttcctt
    12181 ctcgccgtaa ggttcaacat cgttttcttc tttttctatt ttgtaaatat cacgttctgt
    12241 gacaataggt ttcattaaat atagaatatt tggtttattt aaaccacgtc gtttttgaat
    12301 gagtagacca tcgttttcta accctttttt tatttttgtt aacttttctt ttttacaatt
    12361 taagatttcc attagctttt catttgaata aacaaagtaa atgttaccat cttcatcaac
    12421 ccagctattt ttaatagata aatttaagcg atcacgtaag atagcatagg ctattttcat
    12481 atcattagtt agatttttat aattttcact ggtaaagaat actttaggga tttgataaaa
    12541 tctttctttg taattttctt gcactgtaaa atattgattt tgcatgacaa aaaacctctt
    12601 tccatatttt agtttggtaa gaggtaataa aacgttttta tttagcttta catttattta
    12661 atgatttagg tgcttgcact aagatattat tcattatata atacaagtat cgaaaattat
    12721 ataaataaac agtttctaaa cgttttatta ccaaaccccg actattacca gtagttgggg
    12781 tttttgttat attaagttta ttatagcact ttaaataaac ttataaaagg gaaaatatag
    12841 ctttatcaag ggtttataaa cccttgataa atatcataaa cctttcataa acccttgata
    12901 aaaaggttga tggatgttta tttttattta ttaatcactt tattattaag cattataagc
    12961 ttataatatt ttatcaaggg tttataaaca ttttataaag ggttgatgat atgaaaagtg
    13021 tgaaaaagtt gagtgaagaa ttaggggtta gtaagcaaac gatttttaat aatattaagc
    13081 gattgaatat agagacaata aaacaggaaa acacttcgtt tattaaagaa gataccgata
    13141 tagaaaaaat cattcaaaga gtaaatgaaa ataaaaagaa atatggtttt gaaagtacta
    13201 ctgaagacaa acaaaaaaaa gggtcggata atattaattt tgaatcgaaa agcgatgctc
    13261 aaatagtaga aatccttaaa aatcagattg atactttaaa taatcaaatt gaaaaacaag
    13321 aaagtcgtca tgaaacaaca attgaatttt acagaaaaga gttgcaagaa agatcaaaat
    13381 tgcttgaaaa tcagcaagtt ctggcattag agagtaataa aaagattcaa aaattagaaa
    13441 atcaattaga aaaagaaaaa caccttaatt atccctttga tacatctact aacactagac
    13501 aaaacgttga tgcacaagaa aaaacatata ctactaatcc tgtaaatatt aacagagatc
    13561 aggaagaaac taaagaaaca gagattcaat ataaggatat atctggaagt caaagcgacg
    13621 aaagtacaca aggtgaagaa gcaaaaaaag aagacgttcc agctaatcta aatgacaatg
    13681 atagtgatat agaagaaaag agtgaagaaa cggaacctaa aaaggggttt tggagtcgtt
    13741 tgtttggtag ctaaaataat tgattaggca ctaaaagtta taaaatttta gtgtctttta
    13801 tatagtgaga acttctggta atcatcgatt atgtaaacta aagtatttca aataattaac
    13861 tgtattaata ctctcatcat gattgcgtta taaacgttcg ttattgagac aaaatttaat
    13921 agtactacta cacaaaaaga gagtgcgaat tcgcactctc tttttgtgat aaatgaatgg
    13981 ttaagaaaat aattcatcca cagttgtatc aaacaatctt gccaacttaa atgctaatgt
    14041 caatgtagga tcatatttat tgttttctat tgcattaata gtttgcctag acacttttaa
    14101 ttctttactt aaagtttctt gagaaattcc tttttgtttt ctatattctc taattctatt
    14161 tttcattaat ttaaccttta gatgtatatt ttttatttgt agtatagtaa ttaaacagca
    14221 taagaaatag tgctacaatt ataaagaaaa tttctggata ttgagaaaac caagatcctt
    14281 caatacttgg tgacaaacct gtaaatttaa gcactagtgt aaataacata caatatacaa
    14341 tccaaataaa acttttagcc agtatttcaa taaacatttt acgttgtctt tcatcgtatc
    14401 gatcttgttg tcctttttta tctaatgttt ctgaaggtaa atatttgtta gttatagccg
    14461 tccataaaca agaaaaaatt aaaataataa taaaaggaat taaattcatt ataacacctc
    14521 ataaatgtaa aaagtatttg acatttatga gtgtaaatat ttttgataca aatgtcaaat
    14581 actttttaca ttataatata ttttaaaaca ccttattagt aaaacaatta ctatatagaa
    14641 agctgcgaac tcgtactctt ttttcttgta taattattaa aatctcaatt caataatatg
    14701 ttatggaatt tgagacactg tgagagacgc tcatttgcat tgacaatcaa tttatcaaga
    14761 taatccctta tcatgtttaa ttctataaac tgtttgtctt gtgatattaa cttctttcgc
    14821 aatcttacta atcgctttgc cttcttctaa catttccacc acgcgatgat aaataacacg
    14881 tttctgtggg tctttagcat ttggtgaata aagtaaaggg cgccctttat atacgccttt
    14941 ttccttcgca acttgaatcc cttgcgcttg tcgacgttta ctttcatttc tctcttgttc
    15001 tgaaaccatt gctaatattt gaatgattaa atctttcata aacttatcca gtaatggatt
    15061 gccaattact tcattcatca taggtaagct ggtaatcatt aactgaacgt ctttttcttt
    15121 taaataattc acagtttcaa taatttcatc atagttacgt cctaaacgat caatagattc
    15181 cacaacgaat cgatctccca ttctgacaaa attgagtgct tcttgaaata caggtctatt
    15241 ttccactgac tttcccgact gtttctctgt aaagattttc tccacaccaa acgtctttaa
    15301 attatccaat tgtctttcta aattttgatc tatcgatgat actctcgcat aacctataat
    15361 cattcatctt cactccataa taaatataca ccctaatcat acgttttaat tgttataatt
    15421 tcaacaattc tttttgtatg gttagggtgt actcaaagtg tacatatcta taattaaaac
    15481 ttactaaaaa taaatataga ttttttcttt gtaacagaac cgttcatttg aaggtcatct
    15541 ctatcataaa tatttaaatt aactaaaaat tttaatcatc tgtttaatac ttatgaaatg
    15601 tatttacggt aggtattaaa attgatgaaa tttatataag tgctttcaat gaaaatgacc
    15661 gtttatttat cctcctgatt taaggagtta gaaatttcga aaacgattct ttccatttgt
    15721 tgcatttgtt ccatgtccag atatttaata cattatagaa aaccaaagac catcactaat
    15781 taaacgcagt tttaaaattt catcaggatt gccttcgtct ttaaaatggg cctcttttaa
    15841 tataagttct actctttcat acctaactct ttcaatgcca tcgtttcata cgccgattct
    15901 cctgattcca tagcaaactc agcgataaac attgggaata ctgcattgag ttctattata
    15961 tgctctttca ttcgtggcca tatttcacct ccagctttac cgtatgctgt tataagttgc
    16021 tctaatcctt cgtcgtcgaa tacacgatgg tgtcctataa agtccattga tgggtcggag
    16081 tgggttgctt cagtccagtc tatgagacct gtgacgtttg cttggttatc taccattata
    16141 tgtcctggat gtaagtaccc atgtatcatg gtcgcatgtc gaggccatag ttcgtcgttt
    16201 tctaaccatt gtttccatct gttccataat tcatctgata cgccataagt ttctttaact
    16261 ttattcattc ttctttgaaa gtcatttttt atttcttgta tggttttgat atttatatgc
    16321 tgaacgttaa tgttttcttc tggtatgttg tgtaaatcta cgagtgtttc agctaatgtg
    16381 ttaataaagt tttctggtaa tggtttgtgt tcaatttccc atacataatt ttgtatttct
    16441 ggatctattg tggctgcggg tttacctgta agttttgggt aagcaataag gtcttttgca
    16501 tgtactttcc acttcggtat ttcaaatgaa acattcttct gtaagaagtc taccgtttgt
    16561 ttttcgggtt ttgttcgttt ataaacgtca ggtcttcttg gtagtctcaa aacccattct
    16621 actccatgtt tatcttttcc aaatgcaact tgaaaatcaa gtcccgattc attcaatgag
    16681 attgtttgag gttggatgtg taattgatat ttttctgcac atttaataat ttcattatgt
    16741 cgagtcattt tattggatcc ttatttctat tatatttttc ttgtgaacgt attttttata
    16801 ttgatataaa tgaacctaat attcaatata aattagtgta tagtttttaa gttatatcat
    16861 gaagagattg tcctgttaat tcccattttt tatctgatac atgtttaaca aaccttgtat
    16921 catgtgatgt aaacaaaatg attccaggat atttattcat aaacatttct aatgcttcta
    16981 atgttttaat atctaaaaaa ttagttggtt catccaaaat taacatattc gctttcgttg
    17041 aaaataatac tgctaacgat aatttcgttc tttccccacc actcaaaaca ttacaagaac
    17101 gatcaagtgc ttcatttaaa cccaagttat ttaaaatagc tctactgaat gattctgatg
    17161 aatccgtttc atccattaaa tattgcaata atgaaacgtc acgcatgtct tcataagcaa
    17221 gttgacgata gtatgccatt tgcactttag gagaacaatc aattccctct atttggtggt
    17281 aaatagcttc aagtaaagtt gtcttaccta caccatttgc acctacgagc gctatatttt
    17341 tgccatatgg tatttggaat cgtacttgtg ttagcagttt ttgacttcct ttaaccaatg
    17401 ttagattttg tgcaatgatt ggataattat tatggatatc ataaatttta ttttgtggga
    17461 aattgaattc atgataactt tgtggttttt caacttcttc caaatgttcc attctttttt
    17521 caatatgctt agcttgtttt tgagcagcct tctcaaccgt gcctttttgt tttgatgcac
    17581 ttaaacgatc tggtgctata cttttgtttt tttgttttga tgatgcttgt gccatttgtt
    17641 gcgcttgatt tcgtttagct ttactggctt gggacaatct ttgtttttca cttatatact
    17701 gttcatattg acgttgttgt tctaactgtt cttgttcata ttgattttga tactgtgtgt
    17761 aattaccttt aaacactctt attgtgccat cttcttgtat atcccaaatt gtgtcagcaa
    17821 tttggtctat aagtgctcta tcatgactta ctataattaa agtaccatag taatatttta
    17881 aaatattttt cagataatct ttaccaattt tatccaagtg atttgtaggt tcatctaaaa
    17941 gtaatatcgg actataattt gatatgacat tagctaattt atattttgct ttttcaccac
    18001 cactcatact gtcggttgta tgcataggta tatggagttc actcattaaa ctaccgtcta
    18061 acgtgttaaa atcattttcc acatccatgt taagctgttc aaaatattga atttcgccaa
    18121 ccgtttcaac tgttccagat tctggttttg ttttttgagc aatcatattt agtaatgtgg
    18181 ttttgccagt accattaccc ccaataagtg ctattttttc aaattggtaa gcgtaaagat
    18241 gaccgatgtt aagtgatcgt aaatctgtca atttatgatt gatttggtta aatttaattg
    18301 tatattgttc catatttata cactccttgt atatttaaag gacgtgtacg caaaaaaatg
    18361 agacaggcta ctcccgtctc attcacatta cactttcaaa cacttaaaaa tttattatga
    18421 tgttttagta tttgaaagcg tttattattc ctatcgaagg ataaaaaatg acataagtca
    18481 tcctatgagt gtgtattgcg gacaagcctt tttgaattaa tttagttttg tattcaaaaa
    18541 ggtttatttg agtctcatag aagctgtcat tttaatcctc cttcatcgat aaaatagtaa
    18601 tgttaatata acattttgtt ggttttttta caatatatct ctaagtagaa agattatata
    18661 acaaaagtat aaaaatacaa ttttagattg attatacatc tccttaattt agagtgtact
    18721 cataaaata
//
LOCUS       DAKTIO010000040        18699 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.040, whole genome shotgun sequence.
ACCESSION   DAKTIO010000040 DAKTIO010000000
VERSION     DAKTIO010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18699
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.040"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..1258)
                     /locus_tag="O0293_001021"
     CDS             complement(<1..1258)
                     /locus_tag="O0293_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCY0279914.1"
                     /translation="MKNTLYHQLYEKLKKQIIEGQFKEGDKFYSKRQLSKHLSISQTT
                     VEHAYQLLLDEGYIYSRPRSGYFVSEIESLTILNNQPIPSLFDDDSYKPKASDEAYDY
                     AFNLDEIDTKHFPIELFRKYSKDLYDTNHLNQLRRGHFQGELHLRFQLAFYLFTNRGV
                     ICDPNQIIIGSSTEQLVNQLVDLLYTSTFIIEKPSYPPIKNILDKKQVEYEQIEVEDN
                     GINVDEVIKSQKNIVYITPSHQFPTGYVMDLKKRTQLIQWAQEKEERFIIEDDYDSEF
                     RYFGKPIPAIQGLYSRGEKVIYISTFSKSIFPSCRVAYMVLPYSIMKKYHSQNHIEGN
                     TVPVHMQNLIATFISSGGFERHLNKMRRIYRRKLTYILKRLKPYKEQLDIQGAETGMH
                     FTITVKNGLTLQECLDRANKVKLKLQV"
     gene            1362..2249
                     /gene="pdxS"
                     /locus_tag="O0293_001022"
     CDS             1362..2249
                     /gene="pdxS"
                     /locus_tag="O0293_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HCY0279915.1"
                     /translation="MSKIIGSDRVKRGMAEMQKGGVIMDVVNAEQARIAEEAGAVAVM
                     ALERVPSDIRAAGGVARMANPKIVEEVMNAVSIPVMAKARIGHITEARVLEAMGVDYI
                     DESEVLTPADEEYHLRKDQFTVPFVCGCRNLGEAARRIGEGAAMLRTKGEPGTGNIVE
                     AVRHMRQVNSEVSRLTVMNDDEIMTFAKDIGAPYEILKQIKDNGRLPVVNFAAGGVAT
                     PQDAALMMELGADGVFVGSGIFKSEDPEKFAKAIVQATTHYQDYELIGRLASELGTAM
                     KGLDINQLSLEERMQERGW"
     gene            2253..2813
                     /gene="pdxT"
                     /locus_tag="O0293_001023"
     CDS             2253..2813
                     /gene="pdxT"
                     /locus_tag="O0293_001023"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HCY0279916.1"
                     /translation="MKIGVLALQGAVREHIRHIELSGHEGIAVKKVEQLEEIEGLILP
                     GGESTTLRRLMNLYGFKEALQNSTLPMFGTCAGLIVLAQDIVGEEGYLNKLNITVQRN
                     SFGRQVDSFETELDIKGIATDIEGVFIRAPHIEKVGQGVDILCKVNEKIVAVQQGKYL
                     GVSFHPELTDDYRVTDYFINHIVKKA"
     gene            complement(3021..4235)
                     /locus_tag="O0293_001024"
     CDS             complement(3021..4235)
                     /locus_tag="O0293_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCY0279917.1"
                     /translation="MHILIGIIGIIFFLALAFLFSSDRKNIRWKYVGILLAIQLIFAF
                     ILLKTTMGIKVIGGISNGFNYLLLKAADGVNFVFGGIQYIDPKQPPFFFSVLLPIVFI
                     SAIIGILQYTKILPLIINVLGFLISKINGMGRLESYNAVAAAILGQSEVFISIKKQLP
                     YIPKQRLYTLTASAMSTVSASIIGAYFTLIEPKYVVTAVVLNLFGGFIIASIINPYKV
                     NEEDDKLLVEETEERQQSFFEMLGEYILDGFKVAVIVGAMLIGYIAIIALLNGIVSNI
                     FSTVSGGAISWDFQTLIGFVFAPFAFLVGVPWQDAVQAGSVMATKLLSNEFVAMQALG
                     KLSDLSEHAKGVTSVFLVSFANFSSIGIISGAIKSLNDKKGDTVARFGLKLLFGATLV
                     SFISAAIAGFFI"
     gene            4393..4854
                     /locus_tag="O0293_001025"
     CDS             4393..4854
                     /locus_tag="O0293_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HCY0279918.1"
                     /translation="MHNMSDIIEQYIKRLFEESNEDVVEIQRANIAQRFDCVPSQLNY
                     VIKTRFTNEHGYEIESKRGGGGYIRITKIENKDATGYINHLLQLIGPSISQQQAYYII
                     DGLLDKMLINEREAKMIQAVIDRETLSMDMVSRDIIRANILKRLLPVINYY"
     gene            4873..5439
                     /locus_tag="O0293_001026"
     CDS             4873..5439
                     /locus_tag="O0293_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HCY0279919.1"
                     /translation="MLCENCQLNEAELKVKVTSKNKTEEKMVCQTCAEGHHPWNQANE
                     QPEYQEHQDNFEEAFVVKQILQHLATKHGINFQEVAFKEEKRCPSCHMTLKDIAHVGK
                     FGCANCYATFKDDIIDIVRRVQGGQFEHVGKTPHSSHKKIALKRKIEEKNEYLKKLIE
                     IQDFEEAAIVRDEIKALKAESEVQHDDA"
     gene            5429..6436
                     /locus_tag="O0293_001027"
     CDS             5429..6436
                     /locus_tag="O0293_001027"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HCY0279920.1"
                     /translation="MTHNIHDNISQWMKSNEETPIVMSSRIRLARNLENHVHPLMYAT
                     ENDGFRVINEVQDALPNFELMRLDQMDQQSKMKMVAKHLISPELIKQPAAAVLVNDDE
                     SLSVMINEEDHIRIQAMGTDTTLQALYNQASSIDDELDRSLDISYDEQLGYLTTCPTN
                     IGTGMRASVMLHLPGLSIMKRMTRIAQTINRFGYTIRGIYGEGSQVYGHTYQVSNQLT
                     LGKSELEIIETLTEVVNQIIHEEKQIRQKLDTYNQLETQDRVFRSLGILQNCRMITME
                     EASYRLSEVKLGIDLNYIELQNFKFNELMVAIQSPFLLDEEDDKSVKEKRADILREHI
                     K"
     gene            6450..8906
                     /locus_tag="O0293_001028"
     CDS             6450..8906
                     /locus_tag="O0293_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCY0279921.1"
                     /translation="MLFGRLTERAQRVLAHAQEEAIRLNHSNIGTEHLLLGLMKEPEG
                     IAAKVLESFNITEDKVIEEVEKLIGHGQDHVGTLHYTPRAKKVIELSMDEARKLHHNF
                     VGTEHILLGLIRENEGVAARVFANLDLNITKARAQVVKALGNPEMSNKNAQASKSNNT
                     PTLDSLARDLTVIAKDGTLDPVIGRDKEITRVIEVLSRRTKNNPVLIGEPGVGKTAIA
                     EGLAQAIVNNEVPETLKDKRVMSLDMGTVVAGTKYRGEFEERLKKVMEEIQQAGNVIL
                     FIDELHTLVGAGGAEGAIDASNILKPALARGELQCIGATTLDEYRKNIEKDAALERRF
                     QPVQVDEPSVVDTVAILKGLRDRYEAHHRINISDEAIEAAVKLSNRYVSDRFLPDKAI
                     DLIDEASSKVRLKSHTTPNNLKEIEQEIEKVKNEKDAAVHAQEFENAANLRDKQTKLE
                     KQYEEAKNEWKNAQNGMSTSLSEEDIAEVIAGWTGIPLTKINETESEKLLSLEDTLHE
                     RVIGQKDAVNSISKAVRRARAGLKDPKRPIGSFIFLGPTGVGKTELARALAESMFGDD
                     DAMIRVDMSEFMEKHAVSRLVGAPPGYVGHDDGGQLTEKVRRKPYSVILFDEIEKAHP
                     DVFNILLQVLDDGHLTDTKGRTVDFRNTIIIMTSNVGAQELQDQRFAGFGGSSDGQDY
                     ETIRKTMLKELKNSFRPEFLNRVDDIIVFHKLTKEELKEIVTMMVNKLTNRLSEQNIN
                     IIVTDKAKDKIAEEGYDPEYGARPLIRAIQKTIEDNLSELILDGNQIEGKKVTVDHDG
                     KEFKYDIAEQTSETKTPSQA"
     gene            9391..10755
                     /gene="radA"
                     /locus_tag="O0293_001029"
     CDS             9391..10755
                     /gene="radA"
                     /locus_tag="O0293_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCY0279922.1"
                     /translation="MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPK
                     HGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRVLGGGIVSGSLVLIGGDPGIGKS
                     TLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
                     EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEG
                     QIAGPRLLEHMVDTVLYFEGDEHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNP
                     SEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPRRMATGIDHNRLSLL
                     MAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGE
                     VGLTGEVRRVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEVLSF
                     ALHS"
     gene            10780..11853
                     /locus_tag="O0293_001030"
     CDS             10780..11853
                     /locus_tag="O0293_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017328539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HCY0279923.1"
                     /translation="MNIVKLMVIIIYLIIGSALGIIIIPEIANDLGLQNSSFLKNHYV
                     DGIIGSIFMFLIFGVFIRRVTNAIKGLEHFIMRRSAVEILFATIGLIIGLLISVMVSF
                     ILESIGNSIFNHFIPVIITILLCYFGFQFGLKKRDEMLMFLPENIARSMSQHTKSATP
                     KIIDTSAIIDGRILEVIRCGFIDGNILIPQGVINELQIVADSNDSVKREKGKRGLDIL
                     NELYDLDYPTKVIHPTKTHSDIDTMLLKLAKQYHASIITTDFNLNKVCHVHGIKALNV
                     NDLSEAIKPNVHQGDQLHILLTKMGKEPGQAVGYLDDGTMVVVDNAKNLIGSHVNLEV
                     VSLLQTSSGRIVFAKKIEDTVSL"
     gene            12410..13864
                     /locus_tag="O0293_001031"
     CDS             12410..13864
                     /locus_tag="O0293_001031"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCY0279924.1"
                     /translation="MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIE
                     DTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAE
                     DKAYKCYMTEEELEAEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVP
                     QNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMQISDVIRGDDH
                     ISNTPKQIMIYEAFGWEPPRFGHMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEA
                     LFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDT
                     ETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMP
                     ALGEEEQQVINGEQVPELMTHLFSKLEALEPFEAAEIKKTIKEVQKETGIKGKQLFMP
                     IRVAVTGQMHGPELPNTIEVLGKEKVLNRLKQYK"
     gene            14287..14934
                     /gene="cysE"
                     /locus_tag="O0293_001032"
     CDS             14287..14934
                     /gene="cysE"
                     /locus_tag="O0293_001032"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCY0279925.1"
                     /translation="MILLKRMRDDIKMVFEQDPAARSTLEVITTYAGLHAVWSHLIAH
                     KLYNQKKYVAARAISQISRFFTGIEIHPGAKIGKRLFIDHGMGVVIGETCTIGDNVTI
                     YQGVTLGGTGKERGKRHPDIGDNVLIAAGAKVLGNIKINSNVNIGANSVVLQSVPSYS
                     TVVGIPGHIVKQDGVRVGKTFDHRHLPDPIYEQIKHLERQLEKTRNGEIQDDYII"
     gene            14918..16318
                     /locus_tag="O0293_001033"
     CDS             14918..16318
                     /locus_tag="O0293_001033"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCY0279926.1"
                     /translation="MITLYNTLTRQKEVFKPIEPGKVKMYVCGPTVYNYIHIGNARPA
                     INYDVVRRYFEYQGYNVEYVSNFTDVDDKLIKRSQELNQSVPEIAEKYIAAFHEDVGA
                     LNVRKATSNPRVMDHMDDIIQFIKDLVDQGYAYESGGDVYFRTRKFEGYGKLSHQSID
                     DLKVGARIDAGEHKEDALDFTLWKKAKPGEISWDSPFGEGRPGWHIECSVMAFHELGP
                     TIDIHAGGSDLQFPHHENEIAQSEAHNHAPFANYWMHNGFINIDNEKMSKSLGNFILV
                     HDIIKEVDPDVLRFFMISVHYRSPINYNLELVESARSGLERIRNSYQLIEERAQIATN
                     IENQQTYIDQIDAILNRFETVMNDDFNTANAITAWYDLAKLANKYVLENTTSTEVIDK
                     FKAVYQIFSDVLGVPLKSKNADELLDEDVEKLIEERNEARKNKDFARADEIRDMLKSQ
                     NIILEDTPQGVRFKRG"
     gene            16311..16715
                     /locus_tag="O0293_001034"
     CDS             16311..16715
                     /locus_tag="O0293_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HCY0279927.1"
                     /translation="MDNQQDNHIKLLNPLTLAYMGDAVLDQYVRTYIVLKLKSKPNKL
                     HQMSKKYVSAKSQAQTLEYLMEQEWFTDEEMDILKRGRNAKSHTKAKNTDVQTYRKSS
                     AIEAVIGFLYLEKREERLEALLNKIITIVNER"
     gene            16723..17469
                     /gene="rlmB"
                     /locus_tag="O0293_001035"
     CDS             16723..17469
                     /gene="rlmB"
                     /locus_tag="O0293_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCY0279928.1"
                     /translation="MEDTVIVGRHAVREAIITGHPINKILIQEGIKKQQINEILKNAK
                     DQKIIVQTVPKSKLDFLANAPHQGVAALIAPYEYADFDQFLKQQKEKEGLLTVLILDG
                     LEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVIRVTNLAKT
                     IDELKDNGFWVAGTEANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCDFYIKIPM
                     VGHVNSLNASVAASLMMYEVFRKRHDVGEI"
     gene            17469..17993
                     /locus_tag="O0293_001036"
     CDS             17469..17993
                     /locus_tag="O0293_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCY0279929.1"
                     /translation="MKERYLIIDGYNMIGQSPTLSAIAKENLEEARMQLIDAIANYNA
                     VISDEIICVFDAYDQSGVEREYMYHGVKTIFTKEKETADSFIERYVYELYDKHTKHIT
                     VVTSDMSEQHAIFGSGAYRISSREMWRDLKENEIDVSKSLDDISENKPRTRIPLSSEI
                     LAEFEKIRRGHHKK"
     gene            18074..18643
                     /locus_tag="O0293_001037"
     CDS             18074..18643
                     /locus_tag="O0293_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HCY0279930.1"
                     /translation="MKYDLTTQDSTIKRNNAINDKDFEKLVMDLQPLIIRRIKTFGFN
                     HYDLEDLYQEILIRMYRSVQTFDFSGEQPFTNYVQCLITSVKYDYLRKYLATNKRMDN
                     LINEYRVTYPCAIKRFDVENNYLNKLAIKELICQFKYLSAFEKDVMYLMCEQYKPREI
                     AQLMHVKEKVIYNAIQRCKNKIKRYFKMI"
BASE COUNT         6888 a         2557 c         3564 g         5690 t
ORIGIN      
        1 aaacttgtaa ttttaacttt actttgtttg ctctatcaag acactcttgt aatgttaatc
       61 cattttttac tgtaattgta aaatgcatac ccgtttcagc accttgaata tcaagctgct
      121 ctttgtaagg tttcaatctt tttaaaatat aggttagttt tctacgataa attcgtctca
      181 ttttatttaa atgcctttca aaaccaccgg aagatataaa cgttgcaata aggttttgca
      241 tatgaacagg tacagtgttg ccttcaatgt gattttgaga atgatatttt ttcattatag
      301 aatagggtaa caccatatat gcaactcgac agctaggaaa aatagacttt gaaaatgtac
      361 tgatataaat cactttttct cctcttgaat atagaccttg aattgctgga atgggtttgc
      421 cgaaatatct aaactcggaa tcataatcat cttctataat aaatcgttct tctttttctt
      481 gagcccattg tattaattga gttcgttttt ttaagtccat cacatatcca gttggaaatt
      541 gatgggaagg cgttatatat actatatttt tttgtgattt aataacttca tctacgttta
      601 ttccattatc ttcaacttca atttgttcat attcaacttg ttttttatct aaaatatttt
      661 tgattggtgg ataactaggt ttttcgataa taaatgttga agtataaagt aaatcgacta
      721 attgatttac taattgttcg gtagatgagc caattataat ttgattagga tcacaaatta
      781 cgccacgatt agtaaataaa taaaatgcca gttgaaaccg caaatgtaat tctccttgaa
      841 aatgtcctct acgtaattga tttaaatgat ttgtatcata aagatctttg gaatactttc
      901 tgaaaagttc tatagggaaa tgtttcgtat ctatttcatc caaattaaaa gcataatcat
      961 aagcttcatc actcgctttt ggtttatatg aatcatcatc aaaaagagag gggataggtt
     1021 gattgtttaa aattgttaaa gattcaattt cggacacaaa atatccagag cgaggtcttg
     1081 aataaatgta accttcgtct aatagaagtt gatatgcatg ctctacggtt gtttggctaa
     1141 tagataaatg tttgcttaat tgtcttttag aataaaattt atcgccttct ttaaattgac
     1201 cttcaattat ttgttttttt aatttttcat aaagttgatg gtataaagtg tttttcaatt
     1261 ttataactga cctcctaaat ttatcttatt ttgtaccttt ttaaatatca gtttatacat
     1321 tacaatgtat ttaatcaact tgaaaagggg ttttatgtat aatgagtaaa attattggat
     1381 cagacagagt caaaagaggt atggctgaaa tgcaaaaagg cggcgttatt atggatgtcg
     1441 ttaatgctga gcaagcaaga attgcagaag aagctggcgc ggtagcagtt atggcattag
     1501 aacgagtacc ttctgatatt agagctgctg gtggtgttgc acgtatggca aaccctaaaa
     1561 ttgtagaaga agtaatgaat gctgtttcta ttccagtcat ggctaaagca cgtattggtc
     1621 atatcactga agcaagagta ttagaggcga tgggtgttga ctatattgat gaatcagaag
     1681 tgttaacacc agcagatgag gaatatcact taagaaaaga tcaatttaca gtaccatttg
     1741 tatgtggatg tcgtaattta ggtgaagctg cgcgtagaat tggtgaaggt gctgctatgt
     1801 tacgtactaa aggtgaacca ggtacaggta atattgttga agctgtaaga catatgagac
     1861 aagttaattc agaagttagt cgattgactg taatgaatga tgatgagatt atgacttttg
     1921 cgaaagatat cggtgcgcct tatgaaattt taaaacaaat taaagacaat ggtcgtttac
     1981 cggtagttaa ctttgcagct ggtggcgttg cgactcctca agatgctgct ttaatgatgg
     2041 aattaggtgc tgacggtgta ttcgttggat caggtatttt taaatcagaa gatccagaaa
     2101 aatttgctaa agcaattgtt caagcaacaa cacattacca agactatgaa ctaattggaa
     2161 gattagcaag tgaacttggc actgctatga aaggtttaga tatcaatcaa ttatcattag
     2221 aagaacgtat gcaagagcgt ggttggtaag atatgaaaat aggtgtatta gcattacaag
     2281 gtgcagtacg tgaacatatt agacatattg aattaagtgg tcatgaaggt attgcagtta
     2341 aaaaagttga acaattagaa gaaatcgagg gcttaatatt acctggtggc gagtctacaa
     2401 cgttacgtcg attaatgaat ttatatggat ttaaagaggc tttacaaaat tcaactttac
     2461 ctatgtttgg tacatgcgca ggattaatag ttctagcgca agatatagtt ggtgaagaag
     2521 gataccttaa caagttgaat attactgtac aacgaaactc attcggtaga caagttgaca
     2581 gctttgaaac agaattagat attaaaggta tcgctacaga tattgaaggt gtctttataa
     2641 gagccccaca tattgaaaaa gtaggtcaag gcgtagatat cctatgtaag gttaatgaga
     2701 aaattgtagc tgttcagcaa ggtaaatatt taggcgtatc attccatcct gaattaacag
     2761 atgactatag agtaactgat tactttatta atcatattgt aaaaaaagca tagcttaatg
     2821 tatgctaaat caacgaatta ttgatattta tagatttgtt gagaagaaaa tatctccttc
     2881 aaacttagct ttggaggagt tattttttat gtcaaaatta aaaatgataa aaaataaagc
     2941 tatacataag aaaaaaaccc ttcaaagaga ctgagaatag tcaaaatttt gaaggggtta
     3001 attcgatgtt gatgtatttg ttaaataaag aatccagcga ttgcagctga aatgaaagat
     3061 actagtgttg caccgaataa taatttcaaa ccaaagcggg caactgtatc tccttttttg
     3121 tcattaagtg atttaatcgc acctgaaata ataccgatag agctaaagtt agcaaatgat
     3181 actaagaata cagatgtaac accttttgcg tgttcagata aatcactaag tttaccaagt
     3241 gcttgcattg ctacaaattc gttagataat agttttgtcg ccataactga accggcttga
     3301 actgcatctt gccatggcac accgactaag aatgcaaatg gtgcaaagac aaaaccaatt
     3361 aatgtttgga aatcccaaga aatagcgcca cctgaaactg tactaaagat attgcttaca
     3421 attccattta atagagcgat aatggcaatg tatccgatta acattgcgcc tacaatgaca
     3481 gctactttaa atccatctaa aatatattct cctagcattt cgaagaatga ttgttgtctt
     3541 tcttcagttt cttcaactaa taatttgtca tcttcttcat taactttata agggttaata
     3601 attgaagcga tgatgaaacc accaaataag tttaagacaa cagccgttac aacatattta
     3661 ggttcaatta aggtaaagta tgcaccgata attgaagcag aaacagtcga cattgctgaa
     3721 gctgttaatg tgtataaacg ttgcttaggt atgtatggta attgtttttt aattgaaata
     3781 aatacttcag attgtcccaa aattgctgca gcaactgcat tgtatgattc taaacgtccc
     3841 ataccattaa ttttagaaat taagaatcct aaaacattaa tgattaaagg taaaatcttt
     3901 gtgtattgaa ggataccgat aatcgctgaa ataaatacga taggtaataa tacactgaag
     3961 aagaatggtg gttgcttagg atcgatatat tgaataccac cgaatacaaa gttaacacca
     4021 tctgctgctt ttaataataa gtagttaaaa ccgtttgaaa taccaccaat aaccttgatt
     4081 cccattgtag ttttaagcaa gataaatgca aagataagct gaattgcaag taaaattcct
     4141 acatatttcc agcgaatatt tttcctgtct gagctaaata gaaacgcaag tgctaaaaag
     4201 aagataattc cgataatccc aattagaata tgcatatatt tctcattcct ttagtttttt
     4261 ctacaatcta tcatacaata aaatggaagg gctaacatca taaatttttg aaaatataaa
     4321 aacaaattaa ttgaaaaagg tcaaaatagg tcatataata tagtcaaaga aggtcaaaaa
     4381 ggggtgatat acatgcacaa tatgtctgac atcatagaac aatacatcaa acgtttattt
     4441 gaagagtcga atgaagatgt cgttgaaatt cagagagcga atatcgcaca gcgttttgat
     4501 tgcgtaccat cacaattaaa ttatgtaatc aaaacacgat tcactaatga acatggttat
     4561 gaaatcgaaa gtaaacgtgg tggtggtggt tacatccgaa tcactaaaat tgaaaataaa
     4621 gatgcaacag gttatattaa tcatttgctt cagctgattg gaccttctat ttctcaacaa
     4681 caagcttatt atattattga tgggctttta gataaaatgt taataaatga acgtgaagct
     4741 aaaatgattc aagcagttat tgatagagaa acgctatcaa tggatatggt ttctagagat
     4801 attattagag caaatatttt aaaacgtttg ttaccagtta taaattatta ctaaatgaaa
     4861 tgaggtgttg aagtgctttg tgaaaattgt caacttaatg aagcggaatt aaaagttaaa
     4921 gttacaagta aaaataaaac agaagaaaaa atggtgtgtc aaacttgtgc tgagggacac
     4981 catccgtgga atcaagctaa tgaacaacct gaatatcaag aacatcaaga taatttcgaa
     5041 gaagcatttg ttgttaagca aattttacaa catttagcta cgaaacatgg aattaatttt
     5101 caagaagtag cgtttaaaga agaaaaacgt tgcccatcat gtcatatgac tttgaaagat
     5161 attgcacatg ttggtaaatt tgggtgtgct aattgttatg caacatttaa agatgacatc
     5221 attgatatcg tccgcagagt tcaaggtgga caatttgagc acgttggaaa gacaccacat
     5281 tcttcacata aaaagatagc tttaaagcga aaaatcgaag aaaagaatga atatttgaaa
     5341 aaacttattg aaatccaaga ttttgaggaa gcagccattg ttagagatga aattaaagca
     5401 ctaaaagctg agagtgaggt gcaacatgat gacgcataat attcatgata atatcagcca
     5461 atggatgaaa agtaatgaag aaacaccaat tgttatgtct tctagaattc ggttagcgcg
     5521 taatttagaa aatcatgtgc atccactaat gtatgctact gaaaatgatg gatttagagt
     5581 tataaatgag gtacaagatg ccttgccaaa ctttgaatta atgcgtcttg atcaaatgga
     5641 tcaacaaagt aaaatgaaaa tggttgcaaa gcatttgatt agtcctgaac taataaaaca
     5701 accagcagcc gcagtattag tgaatgacga tgaatcttta agtgtcatga taaatgaaga
     5761 ggaccatatt cgtattcaag ctatgggaac tgacacgaca ttacaggctt tatataatca
     5821 agcttcatca attgatgatg aattagatcg aagccttgat ataagttatg atgaacaact
     5881 tggttattta actacatgtc ctaccaatat aggtactggt atgagagcaa gcgtgatgct
     5941 acatttgcca ggtctatcta ttatgaaaag aatgacacgg attgctcaaa ccattaatcg
     6001 ttttggatat acaatcagag gtatttacgg tgaaggttcg caagtttatg gacatactta
     6061 tcaagtatcc aaccaactta cacttggtaa atctgagtta gaaatcatag aaacattaac
     6121 agaagttgtt aatcaaatca ttcatgaaga aaaacaaata cgacaaaagt tagacactta
     6181 taatcaatta gaaacacaag accgtgtttt tcgctcgcta ggtattttac aaaactgtag
     6241 aatgataact atggaagagg cttcttatag attaagcgaa gttaaacttg gtatagattt
     6301 aaattacatt gaattacaaa actttaaatt taatgaattg atggtagcta tacagtcacc
     6361 atttttatta gatgaagaag atgacaaatc tgtaaaagaa aaacgagcag atatactaag
     6421 agaacatata aagtaggagg tcattattta tgttatttgg tagattaact gagcgtgcac
     6481 agcgcgtatt agcacatgcc caagaagaag caattcgttt aaatcattca aatataggaa
     6541 cagaacacct attattgggg ttaatgaaag aacctgaagg aattgctgca aaagtattag
     6601 aaagttttaa tatcactgaa gataaagtaa ttgaagaagt tgaaaaatta atcggacatg
     6661 gtcaagatca tgttggtaca ttgcattata cacctagagc taaaaaagtc attgaattat
     6721 cgatggatga agctagaaaa ttacatcaca attttgttgg aacggaacat attttattag
     6781 gcttgattcg tgaaaatgaa ggtgttgcag caagagtttt tgcaaatcta gatttaaata
     6841 ttactaaagc acgtgcacaa gttgtgaaag ctttaggaaa ccctgaaatg agtaataaaa
     6901 atgcacaagc tagtaagtca aataatactc caactttaga tagtttagct cgtgacttaa
     6961 cagtcattgc caaagacggt acattagatc ctgttatagg acgtgataaa gaaattacac
     7021 gtgtaattga agtattaagt agacgtacga aaaacaatcc tgtgcttatt ggagagccag
     7081 gtgttggtaa aactgctatt gctgaaggtt tagcgcaagc catagtgaat aatgaggtac
     7141 cagagacatt aaaagataag cgtgttatgt ctttagatat gggaacagta gttgcaggta
     7201 ctaaatatcg tggtgaattt gaagagcgtc tgaaaaaggt tatggaagaa atccaacaag
     7261 caggtaatgt catcctattt attgatgagt tgcatacttt agttggtgct ggtggtgctg
     7321 aaggtgctat cgatgcttcg aatattttga agccggcatt agcacgtggt gaattacaat
     7381 gtattggtgc tactacatta gatgaatatc gcaaaaatat tgaaaaagac gcggctttag
     7441 aacgtcgttt ccaacctgta caagttgatg aaccttcagt agtagataca gttgctattt
     7501 taaaaggatt aagagatcgt tacgaagcac accatcgtat taatatttca gacgaagcta
     7561 ttgaagcagc tgttaaatta agtaacagat acgtttcaga tcgtttctta ccagataaag
     7621 caattgattt aattgatgaa gcaagttcta aagtaagact taagagtcat acgacaccta
     7681 ataatttaaa agaaattgaa caagaaattg aaaaagttaa aaatgaaaaa gatgccgcag
     7741 tacatgctca agagtttgaa aatgctgcta acctgcgtga taaacaaaca aaacttgaaa
     7801 agcaatatga agaagctaaa aatgaatgga agaatgcaca aaatggcatg tcaacttcat
     7861 tgtcagaaga agatattgct gaagttattg caggatggac aggtatccca ttaactaaaa
     7921 tcaatgaaac agaatctgaa aaacttctta gtctagaaga tacattacat gagagagtta
     7981 ttgggcaaaa agatgctgtt aattcaatca gtaaagcggt tagacgtgcc cgtgcagggt
     8041 taaaagatcc taaacgacca attggtagct ttatcttcct tggaccaact ggtgttggta
     8101 aaactgaatt agctagagct ttagctgaat caatgtttgg cgatgatgat gcgatgatcc
     8161 gtgtagacat gagtgaattt atggaaaaac acgcagtgag ccgattagtt ggtgctcctc
     8221 caggatatgt tggtcatgat gatggtggac aattaactga aaaagttaga cgtaaaccat
     8281 attctgtaat tttatttgat gaaattgaaa aagctcatcc agatgtattt aatattctat
     8341 tacaagtttt agatgatgga catttgacag atacaaaagg acgtacagtt gatttcagaa
     8401 atacaattat cataatgaca tcaaacgttg gggcacaaga attacaagat caacgatttg
     8461 ctggattcgg tggttcaagt gatggacaag attatgaaac aattcgaaaa acgatgttaa
     8521 aagaattaaa aaattcattc cgtccagaat ttttaaaccg tgtagatgat atcattgtat
     8581 tccataaact aacaaaagaa gaattaaaag aaattgtaac aatgatggtt aataaattaa
     8641 caaatcgatt atctgaacaa aacataaata ttattgtaac tgataaagcg aaagacaaaa
     8701 tcgcagaaga aggatatgat ccagaatatg gtgcaagacc attaattaga gcgatacaaa
     8761 aaactatcga agataattta agtgaattaa tattagatgg taatcaaatt gaaggtaaga
     8821 aagttacagt agatcatgat ggtaaagagt ttaaatatga cattgctgaa caaacttcag
     8881 aaactaaaac accatcgcaa gcataattat aaaacagtcc aaaacaaatt aaagttttgg
     8941 gctgtttttt tagtagcatt gaactataga aattcgtgaa agtatccatc aacgaaacaa
     9001 tctaataaaa caatcatcaa aggatagtta agaattatat gtaacaagtt aatgagccta
     9061 cagcgatagc ataaaggtat gaatttttat aaaggttttt tgtttgaaga tactagcaac
     9121 ttacgtcaaa ataaaatagg tctatataat attgtgataa atctagagaa aagagtttac
     9181 taagaaatta ttaagatttt atctttgaaa gataccgact atcaacatga tgtaagttta
     9241 ttttataata ttcataaaaa ataaatctgg taaaacagtt tgcgttagta gtcatgttaa
     9301 aatacaaaca aatgtaactc acatttaatt tgtcataatg ggaatgtgcg tttaaaatag
     9361 atttgttcaa aggaaagtgg aggtgcaatt ttggccaaga aaaaagtgat ttttgaatgt
     9421 atggcttgtg gttatcaatc tcctaaatgg atggggaaat gtcctaattg tggcgcttgg
     9481 aatcaaatgg aggaaattgt tgaaaaagca gccaatccta aacatggagt taaaaccaag
     9541 gaattagcag gtaaagtaca aaaattaaat agtattaaac atgaaacaac gccgagagtg
     9601 ttaacagatt cagcagaatt caaccgtgta ttaggtggag gtattgtgag cggatcgtta
     9661 gtacttattg gtggggatcc aggtattggt aagtcaacgt tacttttaca aatttgtgca
     9721 tcgttatctc aaaagaaaaa agtactatat attactggag aagaatcgct tagtcagact
     9781 aaattacgtg cagagcgatt agatgaagat tcaagtgaat tgcaagtatt agctgaaaca
     9841 gatcttgaag ttatttatca aacagtaaaa gaagaacaac ctgatttatt agtagtggat
     9901 tcgattcaaa caatatatca tcctgaaatc agctctgcgc caggttctgt ttcacaagtt
     9961 cgtgaaagta cacaaagttt aatgaatatt gctaaacaaa tgaacattgc aacttttata
    10021 gtgggtcatg taacgaaaga aggtcaaatt gctggcccaa gattgctaga acacatggtt
    10081 gatactgtgc tttattttga aggcgatgaa caccacgcat atcgaatttt gcgagctgtt
    10141 aaaaaccgtt ttggttcaac gaatgaaatg ggaatcttcg aaatgaagca aagtggatta
    10201 aaaggtgtaa ataatccatc tgaaatgttt ttagaagaac gttcaacaaa tgttccaggt
    10261 tcaacaattg ttgcaaccat ggagggaacc agaccacttt taatagaagt tcaagcgctg
    10321 gtaactccaa cgacttttaa caatccgaga cgaatggcaa cagggattga tcataatcga
    10381 ttaagtttgt tgatggctgt tttggaaaag aaagaaaatt atctattaca acaacaagat
    10441 gcttatatca aagtagctgg cggtgtaaag ttaacggagc cagcagttga tttaagtgta
    10501 attgtagcaa ctgcatctag ctttaaagat aaagctgtcg acggattaga ttgctatatt
    10561 ggagaagttg gtttaacggg tgaggtacgt cgtgtatctc ggatagaaca acgcgtgcaa
    10621 gaggctgcaa aactaggttt caaacgtgta attattccta aaaataatat aggcggatgg
    10681 acatatcctg aaggtataca agtaataggt gtaactactg tacatgaagt attgtcattt
    10741 gctcttcatt cataaaacat caagaaagga ggacattgtg tgaatatcgt taaactaatg
    10801 gttattatta tttacttaat tattgggagc gcattaggaa taattattat tcctgaaatt
    10861 gcaaatgatc ttggattaca aaactccagc tttttaaaaa atcactatgt agatggcatt
    10921 atcggtagta tttttatgtt cttaattttt ggtgtattta ttagacgagt tactaacgct
    10981 ataaaaggtt tagaacattt tattatgcgt agaagtgctg ttgaaatact attcgcaaca
    11041 ataggtttaa taatcggatt acttatttct gttatggtgt cgtttatatt agaatcaatt
    11101 ggcaactcta tttttaatca tttcattcct gtcataatta cgatattact atgttatttc
    11161 ggtttccaat ttggccttaa aaaacgagat gaaatgttaa tgtttttacc tgagaatata
    11221 gcgcgttcca tgtcacaaca tactaaaagt gctacgccaa aaattatcga cacaagcgca
    11281 attattgatg gtcgtatttt agaagtcatt cgttgcggtt ttatcgatgg caatatttta
    11341 attccacaag gtgttattaa tgaattacaa attgttgcag attcaaatga cagtgttaaa
    11401 cgtgaaaagg gtaaaagagg cttagatatt ttaaatgaat tgtatgattt agactatcct
    11461 acaaaggtta tacatccaac taaaacacat agtgatattg atacgatgtt attaaaatta
    11521 gcaaaacaat atcatgcaag tattataacg acagatttca acctaaataa agtttgtcat
    11581 gtacatggta tcaaagcatt aaatgttaat gatttatcag aagcaatcaa acctaatgta
    11641 catcaaggtg atcaactgca tattttactg acaaaaatgg gtaaggagcc tggtcaggca
    11701 gtaggatatc tagatgatgg tacgatggta gttgttgata atgctaaaaa tcttattggc
    11761 agtcatgtca atttagaagt agtcagctta ttgcaaacat cttcaggaag aattgttttt
    11821 gctaaaaaaa tcgaagatac agtatcatta taaaaactgt agttgacgaa aacaaaatag
    11881 atattaaaag gtatatgaaa tggcgaagtt tgtaatttga agtataacta taaataaatg
    11941 caaaacatat tagttatcaa aaaatacaaa tagtgctaat atatcattga aacaagttca
    12001 ttaagtgtta aattttagat attaataaag ttattcttat agcaatcaag acatacaaaa
    12061 ttagaatcaa ttttaacgta tttgaatagt taatttgaag tttaatttca cagtacataa
    12121 tattcatttt taagtcgttg aaatgtttat gtagtgcagt cttgattctt agggatagct
    12181 tttttaaagt gttgaaaaaa caacagcttt ctatttaaaa tttaagcata ttacccaggg
    12241 aattatgtta tgattaaaca atgaaattat tttaaggtca tgttaaaaag cattgtttcg
    12301 ttttaacaat aagacgagtt agacatttgt tatgacggtc gaagatagca aatgaacaat
    12361 ttatatgaac taacttgaaa atctgatatt taatttagga gtgaaaaaaa tgagcgatcg
    12421 tataagagta agatatgcac caagtccaac tgggtatctt catattggta atgcaagaac
    12481 agcattattc aattacttgt atgctaaaca ttacaacgga gattttgtga ttcgaattga
    12541 agatactgat aaaaaacgta atttagaaga tggagaaaca tcacaatttg ataatcttaa
    12601 atggttagga ttagattggg atgagtctgt agataaagac aatggctacg gaccatatcg
    12661 tcaatctgaa cgtcaacata tctaccaacc attaatagat cagttactag cagaagataa
    12721 agcatataaa tgctatatga cagaagaaga attagaagct gaacgtgaag cgcaaatcgc
    12781 tcgtggtgaa atgcctcgct atggtggtca acatgcgcat ttgactgaag aacaacgtca
    12841 acaatttgaa gcagaaggac gccaaccatc aattcgtttc cgagtacctc aaaaccaaac
    12901 gtattcattt gatgatatgg taaaaggaaa tatttcattt gattcaaatg gtattggtga
    12961 ctgggttatc gtaaaaaaag atggcattcc aacgtacaat tttgcagtag ctatagatga
    13021 tcattacatg caaatttcag atgtaattcg tggtgatgat catatttcaa acacgcctaa
    13081 acaaattatg atttatgaag catttggctg ggagccacct cgttttggtc atatgtcatt
    13141 aattgttaat gaagaacgta aaaagttaag taaacgtgat gggcaaattt tacaatttat
    13201 tgagcaatat cgtgacttag gttatttacc tgaagcgtta tttaatttta ttgcgttatt
    13261 aggttggtct cctgaaggtg aagaagaaat cttttctaaa gaagaattta tcaaaatctt
    13321 tgatgaaaag cgtttgtcaa aatcaccagc atttttcgat aagcaaaaat tagcatgggt
    13381 taataaccaa tatatgaaac aaaaagatac tgaaacagta ttccaattag cattacctca
    13441 tttaattaaa gcaaatttga ttcctgaggt gccgtcagaa gaggatttat cttggggacg
    13501 caaattaatt gcgctttatc aaaaagaaat gagttatgcc ggtgaaattg tacctttatc
    13561 agaaatgttc tttaaagaaa tgccagctct tggtgaagaa gaacaacaag tgattaatgg
    13621 agagcaagta ccagagttaa tgacgcactt attcagtaaa ttagaagcac ttgaaccatt
    13681 tgaagcggct gaaattaaaa agacaattaa agaagttcaa aaagaaacag gaataaaagg
    13741 caagcaatta tttatgccta ttcgtgttgc tgtaacaggc caaatgcatg gtcctgaatt
    13801 accaaataca attgaagtac ttggtaaaga aaaagtgcta aaccgtttaa aacaatataa
    13861 gtaatgaaat agacaagatc aaggtagtat atacttgaat tttataacgt aaccacatat
    13921 gataaagttg tattaacaaa ttacaacaat atgaataaag tattttatat aaaacgaagg
    13981 attagtaatt aaatttatac gatgcagaga gtgtacggtt gctgtgagta caacgtagaa
    14041 attaatgaat gcaccttcgt aaaatgaatt aaatatataa tgagagtgat gagcattaag
    14101 ttgacttagt ttccttgata atttggaagc gcccgcaata ttattaatgt tattcgctaa
    14161 attcagagtg gaaccgtgcg gaagcgcctc taacaataca acttgtatgt tagtggtgct
    14221 tttttgatat ttaatttcgc aggtacttca attatgtatt tacgtagttc aatcattgag
    14281 gaggaaatga tcttgttaaa aagaatgaga gacgatataa aaatggtatt tgagcaggat
    14341 ccagcggcac gttcaacatt agaagtcatt acaacgtatg caggtttaca tgcagtttgg
    14401 agtcatttga ttgcacataa gttatacaac caaaaaaaat atgttgcagc acgcgcgata
    14461 tctcaaattt caagattttt cacaggtata gaaatccatc caggtgctaa aattggaaag
    14521 cgtctattta tagatcatgg tatgggcgtt gtaataggag aaacatgtac aattggtgat
    14581 aatgtgacaa tctatcaagg cgtgacactt ggtgggacag ggaaagaaag agggaaaaga
    14641 cacccagata taggagacaa tgttttaata gcagccggtg cgaaagtttt aggaaatatt
    14701 aaaataaatt caaatgtaaa tattggtgca aattcagttg ttttacaatc agttccaagt
    14761 tattcaacgg ttgttggtat accaggacat attgttaagc aagatggtgt tcgagttgga
    14821 aaaacatttg atcatcgcca tctacctgat ccaatttatg aacaaattaa gcatttagaa
    14881 cgacaacttg aaaagactag gaatggagag attcaagatg attacattat ataatacgct
    14941 tacacgtcaa aaagaagtgt tcaagcctat agaaccaggg aaagtaaaaa tgtatgtatg
    15001 tggtcctact gtatataact acattcatat tggtaacgca agaccagcaa ttaattatga
    15061 cgtagtgaga cgttactttg aataccaagg atataatgta gaatatgtat caaattttac
    15121 agacgtagat gataaattaa ttaaacgttc tcaagaatta aatcagtctg ttcccgaaat
    15181 tgcagaaaaa tatatcgctg cttttcatga agatgttggt gcgttaaatg ttagaaaagc
    15241 gacttcaaat ccaagggtaa tggaccatat ggatgacatt attcaattta ttaaagattt
    15301 ggtggatcaa ggttatgcat atgaaagtgg tggcgatgtt tactttagaa cacgtaaatt
    15361 tgaaggttat ggtaaattaa gtcatcaatc catagatgac ttaaaagtgg gtgctcgtat
    15421 agatgcagga gagcataaag aagatgcact tgattttaca ttgtggaaaa aagcgaagcc
    15481 tggcgagatt agttgggata gcccatttgg tgaaggtaga ccaggatggc atatagaatg
    15541 ttctgtaatg gcatttcatg agctaggacc tacaattgat atacatgcgg gtggttcaga
    15601 tttacaattt ccacatcatg aaaatgaaat agcacaatca gaagcacata atcatgcgcc
    15661 atttgctaat tattggatgc ataatggttt cattaatatt gataatgaaa aaatgagtaa
    15721 atcactaggc aactttattt tagttcacga tattattaaa gaagttgatc cagatgtact
    15781 aagattcttt atgattagcg tacattatag aagcccaatt aactataatc tagaattggt
    15841 agaatcagca cgtagtggac tagagcgtat tcgcaatagt tatcaattaa ttgaagagcg
    15901 cgcacaaatt gctactaata ttgaaaatca acagacatat attgatcaaa ttgatgcgat
    15961 tttaaatcgt tttgaaacag ttatgaatga tgattttaat acagctaatg caattacagc
    16021 ttggtatgat ttagcaaaac ttgcgaataa atatgtacta gagaacacaa catcaacaga
    16081 agtaattgat aaatttaaag cagtttatca aattttcagc gatgttttag gtgtaccgtt
    16141 aaaatctaaa aatgcagatg aattattgga tgaagatgtt gaaaaattaa tcgaagagcg
    16201 taatgaagca aggaaaaaca aagattttgc acgagcagat gaaattcgag acatgctgaa
    16261 atcacaaaac attatattag aagacacacc tcaaggggtt agatttaaac gtggataatc
    16321 aacaagataa tcacattaaa ttattgaatc cattgacctt agcatatatg ggagacgcag
    16381 tcttagatca atatgtacgt acctatatcg ttttaaagct taaaagtaag cctaataaac
    16441 tacatcaaat gtctaaaaaa tatgtatctg ccaaaagtca ggcgcaaacg ttagaatatt
    16501 taatggagca agaatggttt acagacgaag aaatggatat tttgaagcga gggcgtaacg
    16561 cgaaaagtca tactaaagct aaaaacactg atgttcaaac atatcgtaaa agttcagcga
    16621 tagaagcagt gataggtttt ctttatttag aaaaaagaga agaacgatta gaggcattat
    16681 taaataaaat aataacaata gtaaacgaaa ggtagtgacg atgtggaaga tacggttatt
    16741 gttggtaggc atgctgttag agaagcgatt attactgggc atccgataaa taagatattg
    16801 attcaagaag gtattaaaaa gcaacaaatt aatgaaattt taaaaaatgc aaaagatcaa
    16861 aaaatcattg ttcaaactgt accaaaatct aaattagatt ttttagcaaa tgcaccacat
    16921 cagggtgttg cagcgcttat tgcaccatat gaatatgctg acttcgatca atttttaaaa
    16981 cagcaaaaag aaaaagaagg tttattgaca gtacttatat tagacggctt agaagaccca
    17041 cataacttgg gatcaatttt aagaacagcc gatgcaacgg gagttgatgg tgttattatt
    17101 cctaaacgtc gttcagttac actaacgcaa acagttgcaa aagcctcaac aggtgcaatt
    17161 gaacatgtac cagttattcg agtgacaaat ttagctaaaa ctatcgatga actaaaagat
    17221 aatggctttt gggtagctgg cactgaagct aataatgcaa cagattatag aaatctagaa
    17281 gcggacatgt cattggctat tgtaattggt agcgaaggac agggtatgag tcgcctagta
    17341 agtgataaat gcgattttta tattaagatt ccaatggttg gacatgtaaa cagtttgaat
    17401 gcttcggttg cagcaagttt aatgatgtac gaagtatttc gaaaaagaca tgatgttgga
    17461 gaaatataat gaaagaacgt tacttaatca ttgatggata caatatgata ggacaatcac
    17521 caacgctaag cgccattgca aaagagaatt tagaagaagc tagaatgcaa ttaatagatg
    17581 caattgcaaa ttataatgca gttatttcag atgaaattat ttgtgttttc gatgcttatg
    17641 accaatcggg tgttgaaaga gaatacatgt atcatggcgt taaaacgatt tttaccaagg
    17701 aaaaagaaac agctgatagt ttcatagaac gttatgttta tgaactttat gacaagcata
    17761 ctaagcatat tacagttgta acaagtgata tgagtgagca acatgctatc tttggatcag
    17821 gtgcatatag aatatcatct cgcgaaatgt ggagagattt aaaagaaaat gaaattgatg
    17881 tgagtaaatc attagatgat ataagtgaaa acaagccaag aactcgaatt ccgttatctt
    17941 ctgaaatcct tgcagaattt gaaaaaatac gaagaggaca tcataagaaa tgacatttcc
    18001 gtcatcttga aattttgaat gtaacaatat taaactaacc ttaaatttta gatagaaggg
    18061 gttagtttaa tatttgaaat acgatttgac aactcaagac agtacaatca aacgtaacaa
    18121 tgcaataaat gataaagact tcgaaaagtt agtaatggat ctacaaccat taattattcg
    18181 acgcatcaaa acatttggat ttaatcatta tgatttagaa gacttatatc aagaaatact
    18241 tatacggatg tataggtcgg tccaaacatt tgattttagt ggagagcagc ctttcacaaa
    18301 ttatgttcaa tgtttaatta cgtctgtaaa gtatgattat ttgagaaaat atttagctac
    18361 aaataaaaga atggataatt tgattaatga atatagagtt acgtatccat gtgcaataaa
    18421 gcgttttgat gttgaaaaca attatttgaa taaattagca attaaagagt tgatttgtca
    18481 gtttaagtat ttgagtgcat ttgaaaaaga tgtcatgtat ttaatgtgtg aacaatataa
    18541 gccgagagaa attgctcaat tgatgcatgt aaaagagaaa gtgatttata atgccataca
    18601 acgatgtaaa aataaaataa aacgttattt caaaatgatt tgaaaagcgc cttaggacgt
    18661 gaattgaatt ataacgtgtt acttactgat ggtttgaca
//
LOCUS       DAKTIO010000041        17809 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.041, whole genome shotgun sequence.
ACCESSION   DAKTIO010000041 DAKTIO010000000
VERSION     DAKTIO010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17809
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.041"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(351..524)
                     /locus_tag="O0293_001038"
     CDS             complement(351..524)
                     /locus_tag="O0293_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NETI motif-containing protein"
                     /protein_id="HCY0279931.1"
                     /translation="MKFKVNENETIADCLSRMKMQGYMPVKRIEKPVFQEQKDGTVEV
                     SHQEIVFVGKKIQ"
     gene            complement(505..1107)
                     /locus_tag="O0293_001039"
     CDS             complement(505..1107)
                     /locus_tag="O0293_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HCY0279932.1"
                     /translation="MSFVTENPWLMVLTIFIINICYVTFLTMRTILTLKGYRYIAASV
                     SFLEVLVYIVGLGLVMSNLDHIQNIIAYAFGFSIGIIVGMKIEEKLALGYTVVNVTSA
                     EYELDLPNELRNLGYGVTHYAAFGRDGSRMVMQILTPRKYERKLMDTIKNLDPKAFII
                     AYEPRNIHGGFWTKGIRRRKLKDYEPEELESVVEHEIQSK"
     gene            complement(1378..2199)
                     /gene="nadE"
                     /locus_tag="O0293_001040"
     CDS             complement(1378..2199)
                     /gene="nadE"
                     /locus_tag="O0293_001040"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCY0279933.1"
                     /translation="MSKLQDVIVQEMKVKKRIDSAEEIMELKQFIKNYVQSHSFIKSL
                     VLGISGGQDSTLVGKLVQMSVNELREEGIDCTFIAVKLPYGVQKDADEVEQALRFIEP
                     DEIVTVNIKPAVDQSVQSLKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTD
                     HSAENITGFYTKYGDGAADIAPIFGLNKRQGRQLLAYLGAPKELYEKTPTADLEDDKP
                     QLPDEDALGVTYEAIDNYLEGKPVTPEEQKVIENHYIRNAHKRELAYTRYTWPKS"
     gene            complement(2192..3661)
                     /locus_tag="O0293_001041"
     CDS             complement(2192..3661)
                     /locus_tag="O0293_001041"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCY0279934.1"
                     /translation="MYQLEDDSLMLHNDLYQINMAESYWNDNIHEKMAVFDLYFRKMP
                     FNSGYAVFNGLKRVIDFIEHFGFSESDLEYLKSIGYKDDFLSYLKDLKFTGSIRSMQE
                     GELCFGNEPLLRVEAPLIQAQLIETILLNIVNFHTLITTKASRIRQIASNDKLMEFGT
                     RRAQEIDAALWGARAAYIGGFDSTSNVRAGKLFGIPVSGTHAHAFVQTYGDEYVAFKK
                     YAERHKNCVFLVDTFHTLKSGVPNAIKVAKELGDKINFVGIRLDSGDIAYLSKEARRM
                     LDEAGFTETKIIASNDLDEETITSLKAQGAKVDSWGVGTKLITGYDQPALGAVYKLVA
                     IENEDGSYSDRIKLSNNAEKVTTPGKKNVYRIINKKTGKAEGDYITLENENPYDEQPL
                     KLFHPVHTYKMKFIKSFEAIDLHHNIYENGKLVYQMPTEDESREYLALGLQSIWDENK
                     RFLNPQEYPVDLSKACWDNKHKRIFEVAEHVKEMEEDNE"
     gene            3845..4921
                     /locus_tag="O0293_001042"
     CDS             3845..4921
                     /locus_tag="O0293_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase oxygenase"
                     /protein_id="HCY0279935.1"
                     /translation="MLFKEAQAFIENMYKECHYETQIINKRLHDIELEIKETGTYTHT
                     EEELIYGAKMAWRNSNRCIGRLFWDSLNVIDARDVTDEASFLSSITYHITQATNEGKL
                     KPYITIYAPKDGPKIFNNQLIRYAGYDNCGDPAEKEVTRLANHLGWKGKGTNFDVLPL
                     IYQLPNESVKFYEYPTSLIKEVPIEHNHYPKLRKLNLKWYAVPIISNMDLKIGGIVYP
                     TAPFNGWYMVTEIGVRNFIDDYRYNLLEKVADAFEFDTLKNNSFNKDRALVELNYAVY
                     HSFKKEGVSIVDHLTAAKQFELFERNEAQQGRQVTGKWSWLAPPLSPTLTSNYHHGYD
                     NTVKDPNFFYKKKESNANQCPFHH"
     gene            4941..5735
                     /locus_tag="O0293_001043"
     CDS             4941..5735
                     /locus_tag="O0293_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HCY0279936.1"
                     /translation="MQLYYLGPKGTFSYLACRQYFSENEATFQPKSNFFEVIKAVADD
                     DTSIGVVPIENSIEGTINIVADALAQQDVFAHGEIRLDINFALYGNGTDSISDIKKVY
                     SIAPAISQTTNYIHQHQFDYDYVDSTIQSLTKIENGVAAIAPLGSGEAYGFTPIDTHI
                     EDYPHNVTRFLVIKNQQQFDQNATSLMFLITPMHDKPGLLASVLNTFALFNINLSWIE
                     SRPLKTQLGMYRFFVQADSAITTDIKKVIAILETLDFKVEMIGAFN"
     gene            5807..5914
                     /locus_tag="O0293_001044"
     CDS             5807..5914
                     /locus_tag="O0293_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279937.1"
                     /translation="MVDFLLFVLFLHDAAVYVSLPLVQAIANVTFYFSF"
     gene            complement(5925..7487)
                     /locus_tag="O0293_001045"
     CDS             complement(5925..7487)
                     /locus_tag="O0293_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCY0279938.1"
                     /translation="MAYFNQHQSMISKRYLTFFSKSKKKKPFSAGQLIGLILGPLLFL
                     LTLLFFHPQDLPWKGVYVLAITLWIATWWITEAIPIAATSLLPIVLLPLGHILTPEQV
                     SSEYGNDIIFLFLGGFILAIAMERWNLHTRVALTIINLIGASTSKILLGFMVATGFLS
                     MFVSNTAAVMIMIPIGLAIIKEAHDLQEANTNQTSIQKFEKSLVLAIGYAGTIGGLGT
                     LIGTPPLIILKGQYMQHFGYEISFAKWMIVGIPTVIVLLGITWLYLRYVAFRHDLKYL
                     PGGQTLIKQKLDELGKMKYEEKVVQTIFVLASLLWITREFLLKKWEVTSSVADGTIAI
                     FISILLFIIPAKNTEKHRRIIDWEVAKELPWGVLILFGGGLALAKGISESGLAKWLGE
                     QLKSLNGVSPILIVIVITIFVLFLTEVTSNTATATMILPILATLSVAVGVHPLLLMAP
                     AAMAANCAYMLPVGTPPNAIIFGSGKISIKQMASVGFWVNLISAIIIILVVYYVMPIV
                     LGIDINQPLPLK"
     gene            complement(7692..8789)
                     /locus_tag="O0293_001046"
     CDS             complement(7692..8789)
                     /locus_tag="O0293_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HCY0279939.1"
                     /translation="MYINMKDYGLTGINKTKDTRAIQRALNRGRCKPTTVYIPKGTYD
                     ICKPLTIYGNTTLLLDNETILRRCHSGPLLKNGRRFGFYRGYNGHSHIHIKGGKFDMN
                     GVSYPYNNTAMCIGHAEDIQLIGVTIKNVVSGHAIDACGINGLYIKSCSFEGFIDYSG
                     ERFYSEAIQLDIQVPGAFPKFGTTDGTITKNVIIEDCYFGPSELPEMGSWNRAIGSHA
                     SRHNRYYENIHIRNNIFEDIQGYALTPLKYKDAFIINNKFINCEGGIRYLGVRDGKNA
                     ADVMTGKDLGSQAGINMNIIGNEFKGSMSKDAIHVRNYNNVKHKDVLIVGNTFNNSTQ
                     SIHLEDIDTVFLSPVEAGIQVTTINVDEIKK"
     gene            9161..9721
                     /locus_tag="O0293_001047"
     CDS             9161..9721
                     /locus_tag="O0293_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCY0279940.1"
                     /translation="MTHRALLVVDYSYDFIADDGLLTCGKPGQNIEDFIVSRINDFNY
                     YQDHIFFLMDLHYLHDIHHPESKLFPPHNIVDTSGRELYGKVGKLYETIKAQPNVHFI
                     DKTRYDSFFGTPLDSLLRERSINQVEIVGVCTDICVLHTAISAYNLGYKISVPAEGVA
                     SFNQKGHEWALAHFKNSLGAEVEQHV"
     gene            9774..10703
                     /locus_tag="O0293_001048"
     CDS             9774..10703
                     /locus_tag="O0293_001048"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HCY0279941.1"
                     /translation="MAKTYIFGHKNPDTDAISSAIIMAEFEQLRGNSGAKAYRLGDVS
                     AETQFALDTFNVPAPELLTDDLDGQDVILVDHNEFQQSSDTIASATIKHVIDHHRIAN
                     FETAGPLCYRAEPVGCTATILYKMFRERGFEIKPEIAGLMLSAIISDSLLFKSPTCTQ
                     QDVKAAEELKDIAKVDIQKYGLDMLKAGASTTDKSVEFLLNMDAKSFTMGDYVTRIAQ
                     VNAVDLDEVLNRKEDLEKEMLAVSAQEKYDLFVLVVTDIINSDSKILVVGAEKDKVGE
                     AFNVQLEDDMAFLSGVVSRKKQIVPQITEALTK"
     gene            complement(10898..11071)
                     /locus_tag="O0293_001049"
     CDS             complement(10898..11071)
                     /locus_tag="O0293_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279942.1"
                     /translation="MNVVAVNFKWSFELYMLKVENRAMNRVHNNIYNFNHNEKGRKKT
                     KIYTQHRKYFKKM"
     gene            11123..12502
                     /locus_tag="O0293_001050"
     CDS             11123..12502
                     /locus_tag="O0293_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCY0279943.1"
                     /translation="MNIIEQKFYDSKAFFNTQQTKDISFRKEQLKKLSKAIKSYESDI
                     LEALYTDLGKNKVEAYATEIGITLKSIKIARKELKNWTKTKNVDTPLYLFPTKSYIKK
                     EPYGTVLIIAPFNYPFQLVFEPLIGAIAAGNTAIIKPSELTPNVARVIKRLINETFDA
                     NYIEVIEGGIEETQTLIHLPFDYVFFTGSENVGKIVYQAASENLVPVTLEMGGKSPVI
                     VDETANIKVASERICFGKFTNAGQTCVAPDYILVHESVKDDLITALSKTLREFYGQNI
                     QQSPDYGRIVNLKHYHRLTSLLNSAQMNIVFGGHSDEDERYIEPTLLDHVTSDSAIMQ
                     EEIFGPILPILTYQSLDEAIAFIHQRPKPLSLYLFSEDENATQRVINELSFGGGAIND
                     TLMHLANPKLPFGGVGASGMGRYHGKYSFDTFTHEKSYIFKSTRLESGVHLPPYKGKF
                     KYIKAFFKN"
     gene            complement(12622..13650)
                     /locus_tag="O0293_001051"
     CDS             complement(12622..13650)
                     /locus_tag="O0293_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HCY0279944.1"
                     /translation="MTNGYIGSYTKKNGKGIYRFELNENQSRIDLLETGFELEASTYL
                     VRNNEVLYGINKEGEQCGVASLKIDDNGELHLLNKCLSSKAGTGCYVSISEDKRYLFE
                     AVYGAGIIRMYELNTHTGEIIRLIQELAHDFPTGTHERQDHPHAHYINQTPDGKYVAV
                     TDLGADRIVTYKFDDNGFEFYKESLFKDSDGTRHIEFHDNGKFAYVVHELSNTVSVAE
                     YNDGKFEELERHLTIPENFDGDTKLAAVRLSHDQQFLYVSNRGHDSIAIFKVLDNGQH
                     LELVTITESGGQFPRDFNIASSDDLLVCAHEQGDSVVTVFERNKETGKITLCDNTRVA
                     SEGVCVIF"
     gene            13920..14321
                     /locus_tag="O0293_001052"
     CDS             13920..14321
                     /locus_tag="O0293_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HCY0279945.1"
                     /translation="MTSYEEETDYRNVPRELLDNNIPMGRGMIKWAPFATLPEQFETI
                     QQYIIDQNKITRPVLSDDQLAELNIRLHEALQYAQPVEVKFYNNGFVDSVRLTIYRID
                     AINYEIDGYVYNQQQRQKISIFDILDIVLLP"
     gene            complement(14339..14547)
                     /locus_tag="O0293_001053"
                     /pseudo
     CDS             complement(14339..14547)
                     /locus_tag="O0293_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(14862..15554)
                     /locus_tag="O0293_001054"
     CDS             complement(14862..15554)
                     /locus_tag="O0293_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HCY0279946.1"
                     /translation="MNKAERQNLIITAIQQNKKMTALELAKYCNVSKRTILRDIDDLE
                     NQGVKIYAHYGKNGGYQIQQAQSKIALNLSETQLSALFLVLNESQSYSTLPYKSEINA
                     IIKQCLSLPQTRLRKLLKRMDFYIKFDDTQHMTLPMLFSDILIYCTERNVMLVDHRVD
                     DNIKAENVIFIGLLCKHGHWHAVIYDIAQDKTAELEIENIIDISYSFGKTIQTRDISI
                     DNYHQFLNPIDS"
     gene            15939..16736
                     /locus_tag="O0293_001055"
     CDS             15939..16736
                     /locus_tag="O0293_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HCY0279947.1"
                     /translation="MTDKTLQQIDKLICSWLKQIDNVIPQLIMEMTTETKRHRFDLVT
                     NVDKQIQQQFQQFLATHFPEHQLLAEEKSNEMITNEINHLWIMDPIDGTANLVKQQED
                     YCIILAYFYEGKPMLSYVYDYPHKKLYKAIRGEGAFCNGIKMEEPPSLKLEDAIISFN
                     AQVMNLDTVQDLFDASFSYRLVGACGLDSMRVAKGQFGAHINTNPKPWDIAAQFLFAE
                     LLNLKMTTLDGKAIDHLKGAPFIISNKACHETVLKILNANGGYQKYR"
     gene            16869..>17809
                     /locus_tag="O0293_001056"
     CDS             16869..>17809
                     /locus_tag="O0293_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCY0279948.1"
                     /translation="MKRSSKSKMSLPKKIFLWVFGILVILAIVAVVYVAAKIFITGNK
                     IHNPLDRNHSELRDKKVSLNDGDPFTIALFGVDSDADRKKKGGGERSDSIMILSINPK
                     TKKTEIVSIPRDTRAEIVGRGTTEKIAHAYAYGGPNMAVKSLEKLMNVPIDHYATIDM
                     DGLHNMIDSIGGVDVVSNDTFTVDGVRFTKGQQTHVNGDQALKFIRSRKEEGAGGDFG
                     RQQRQQIVLEAMANKIASPSSITHFNSLMNEIQNNVKTDLTLGDLNTIRSNYKDANDT
                     INKHQLSGQGGIQSDGLYYFIPSEQSKAESTKLLKDNL"
BASE COUNT         5980 a         3030 c         2752 g         6047 t
ORIGIN      
        1 tgaacacaga agctttggga acttctccta gaatgacttt cattaagcat aagtcataag
       61 actatctaga tatgattaag taaactatta atcttattaa aggtctctag gaagaaaaaa
      121 tttcatgcga tgagattgtt ttagaaaaag cgacgatata tataatctaa caataatttt
      181 taaaatatta agaaccccca gcatatgctt tatatagctt gctgggggtt tctcatacaa
      241 tatggatcga gcatcttgca tgtataaaca aataggctat tcaaagtaaa attactcaaa
      301 ctttgaatag cctttcctct tcaaatagtc tttatttaaa ttggattatg ttattggatt
      361 ttcttaccta caaaaacgat ttcttgatgt gatacttcaa ccgtgccatc tttttgctct
      421 tgaaacacgg gtttttcaat acgttttacc ggcatatacc cttgcatttt cattcgtgac
      481 aaacaatcag caattgtttc attctcattt actttgaatt tcatgttcta ctacactttc
      541 cagttcttct ggttcataat ctttaagctt tctacgacga atgcctttag tccagaatcc
      601 accatgtatg tttcgaggtt catacgcaat gataaatgct ttcggatcta aattttttat
      661 cgtatccatc aatttacgtt catattttct tggtgttaaa atttgcatca ccatacgact
      721 accatctcta ccaaacgcag catagtgcgt aacgccatat cctaaatttc gaagttcatt
      781 cggtaaatct aactcatatt ctgctgaagt tacatttaca actgtataac ctaatgccag
      841 tttttcttct attttcatac caacaatgat acctattgaa aaaccaaatg cgtaggcaat
      901 aatattttga atatggtcta aattagacat aaccaaacct aaaccaacga tataaactaa
      961 tacttctaaa aaactaactg atgcagcaat ataacgataa cctttcaacg ttaaaattgt
     1021 tcgcatcgtt aaaaacgtta cataacaaat gttaatgata aatatagtta gtaccattaa
     1081 ccatggattt tctgttacga atgacataag gcgccccact ctctttcaat tacactttaa
     1141 ataccaaact gcttcaacct aaccttaatg aatgcatatt aatataatat agtacgaaaa
     1201 ctctcataaa taaatagcga atttgtcata aataaaaatt tattctcaaa tgaacatact
     1261 tactttagtt catacacttt caatttatac actgaagtag tctagctgaa tgtggtttaa
     1321 tgttattaat tctaactcat atttaattta agtcacacgt tagaagaaaa aattaaatta
     1381 ggattttggc cacgtgtatc ttgtatatgc aagttcacgt ttgtgtgcat ttcgtatata
     1441 atgattttca attacttttt gttcttctgg cgtaactggc ttaccttcta aataattatc
     1501 aatcgcctca taagttacac ctaatgcatc ttcatctgga agctgtggtt tatcatcttc
     1561 taaatcagca gttggcgttt tttcatataa ttcctttggc gcaccaagat acgctaataa
     1621 ttgacgacct tgtcgtttat tcaaaccaaa tataggtgcg atatctgcag caccatcacc
     1681 gtacttcgta taaaacccag ttatattttc agctgaatga tctgttccta ctacaatacc
     1741 ttgtcggttt gaagcaattg aaaattgtac tttcatacgt tcacgcgctt tttcatttcc
     1801 tttttggaaa tctgtaagaa caataccggc ttcttttaat gattgcacac tttgatcaac
     1861 tgcaggctta atattgactg ttactatttc atctggttca atgaatcgca aagcttgctc
     1921 aacttcatca gcatcttttt gaactccata aggtaattta actgcaataa acgtacaatc
     1981 aatgccttct tcacgtaatt cgttaacaga catttgtact agttttccaa ctaatgtaga
     2041 atcctgtcct cctgaaatac ctaacactaa agattttata aatgaatgtg attgtacata
     2101 attttttata aattgcttta attccataat ttcttcagca ctatcgatac gctttttcac
     2161 tttcatttct tgtacaataa cgtcttgtaa tttactcatt atcttcttcc atctccttaa
     2221 cgtgttccgc aacttcaaaa atacgtttat gtttattatc ccaacatgcc ttgcttaaat
     2281 cgactggata ttcttgtgga ttcaggaaac gcttattttc atcccaaata gattgtaatc
     2341 ctagtgctaa atattcacgt gattcatctt ctgttggcat ttgatatact aatttaccat
     2401 tttcataaat attatgatgc aaatcaatgg cttcgaaaga ttttataaat ttcattttat
     2461 aagtatgcac tggatggaat aattttaaag gttgttcatc gtatggattt tcattttcca
     2521 aagtaatata atcgccttct gccttacctg ttttcttgtt tataatgcga tatacatttt
     2581 tcttacctgg cgtcgtaacc ttttcagcgt tatttgataa tttaatacga tcactatatg
     2641 aaccatcttc attttcaata gctacaagtt tatatactgc acctaatgct ggttgatcgt
     2701 atcctgtaat cagctttgta ccaacgcccc aagaatctac ttttgcacct tgtgctttca
     2761 aactcgtaat cgtttcttca tccaaatcat tagacgcgat aattttagtt tcagtaaatc
     2821 ctgcttcatc aagcatacgt cttgcctctt tagataaata agcgatatct ccagaatcta
     2881 atcgaatacc tacaaagtta attttgtcac ctaattcttt tgcaactttt attgcatttg
     2941 gcacgccaga ttttaaagta tggaatgtat ctactaggaa cacacaattt ttatgtcttt
     3001 cagcatattt tttgaaggca acatattcgt ctccataagt ttggacaaat gcatgtgcat
     3061 gtgtaccaga cacaggtata ccaaataatt tccccgccct aacattactt gtagaatcaa
     3121 agcccccgat gtaagcagct ctagcgcccc acaatgctgc atcaatttct tgcgcacgac
     3181 gtgtaccaaa ctccattaat ttatcatttg atgcaatttg acgaattctg ctagcctttg
     3241 ttgtaattaa tgtatggaaa tttacaatgt ttaataaaat tgtttctatt aattgcgctt
     3301 gaatcaatgg tgcttctacg cgtaacaatg gttcgttacc aaagcataat tcgccttctt
     3361 gcatcgaacg gatgctgcct gtgaatttta aatcttttaa atatgataag aaatcatcct
     3421 tgtagccaat agactttaaa tattccaaat cagattctga aaatccaaaa tgttctataa
     3481 aatcaatgac gcgttttaaa ccattaaaaa cagcatagcc actattaaat ggcatttttc
     3541 taaaatacaa atcaaataca gccatttttt catgaatatt atcattccaa taactttcag
     3601 ccatatttat ttgatataag tcattatgta acattaaact gtcgtcttct aattggtaca
     3661 cttgtatctc tccaatcgac ctaaatattt tcttacattt tatcataatt cattttttta
     3721 tatacataag agccccttaa tttccatact tttaattaaa atcaaccaac aatttaatga
     3781 catatacata atttttaaga gtattttaat aatgtagact ataatataaa gcgaggtgtt
     3841 gttaatgtta tttaaagagg ctcaagcttt catagaaaac atgtataaag agtgtcatta
     3901 tgaaacgcaa attatcaata aacgtttaca tgacattgaa ctagaaataa aagaaactgg
     3961 gacatataca catacagaag aagaacttat ttatggtgct aaaatggctt ggcgtaattc
     4021 aaatcgttgc attggtcgtt tattttggga ttcgttaaat gtcattgatg caagagatgt
     4081 tactgacgaa gcatcgttct tatcatcaat tacttatcat attacacagg ctacaaatga
     4141 aggtaaatta aagccgtata ttactatata tgctccaaag gatggaccta aaattttcaa
     4201 caatcaatta attcgctatg ctggctatga caattgtggt gatcctgctg aaaaagaagt
     4261 tacacgctta gcaaatcact taggttggaa aggaaaaggt actaattttg acgtgttacc
     4321 actgatttac caattaccta atgagtcagt taaattttac gaatatccta cttcattaat
     4381 taaagaagta cctattgaac ataatcatta tccaaaatta agaaaattga acttaaaatg
     4441 gtatgcagtc cctatcattt ccaatatgga cttaaaaatc ggtggcattg tatatccaac
     4501 tgcacccttt aacggttggt atatggtaac tgaaattggc gtacgtaact ttattgatga
     4561 ttaccgttac aatttactag aaaaagttgc agatgcgttt gaatttgata cacttaaaaa
     4621 taattcattt aataaagatc gagcacttgt tgaattgaac tatgctgtgt atcattcctt
     4681 taaaaaagaa ggcgtatcaa ttgtcgatca tttgaccgct gcaaagcaat tcgaactatt
     4741 cgaacgtaac gaagcacaac aaggtcgtca agttaccgga aaatggtctt ggctagcacc
     4801 gccattatct ccaacattga cgtcaaatta tcatcacgga tatgacaata cagtaaaaga
     4861 tccaaacttt ttctataaaa agaaagaatc aaatgctaac cagtgccctt tccatcatta
     4921 agttagtaga ggtgtagcat atgcaattat attatttagg ccctaaaggt acattttcat
     4981 atttggcttg tcgtcagtat ttttctgaaa atgaagcaac ttttcaacca aagtcaaatt
     5041 tttttgaagt catcaaggca gttgcagatg atgacacatc gattggtgtt gtccctattg
     5101 aaaattctat agaaggaaca atcaacattg tggcagatgc acttgcacag caagatgtct
     5161 ttgcacacgg tgaaatacgt ttagatatta actttgcatt atatggtaat ggaacagatt
     5221 ccatttctga tattaaaaaa gtgtattcta ttgcaccagc aatcagtcaa acaacaaatt
     5281 acatacatca acatcaattt gactatgatt atgtcgacag tacaattcaa agtttgacta
     5341 aaattgaaaa tggcgttgca gcaattgcac ctttaggtag tggcgaagct tatggtttta
     5401 cacctattga tacacatatt gaagactatc cacataacgt gacacgtttt ttagttataa
     5461 aaaatcagca gcaatttgat caaaatgcga catcattgat gtttttaatt acaccaatgc
     5521 atgataaacc tgggttgtta gcaagtgtgc ttaatacatt tgctttgttt aatattaatt
     5581 tatcctggat tgagtctcgt cctttaaaaa cacaattagg tatgtatcga tttttcgttc
     5641 aggctgattc tgcaattaca accgatataa agaaagtcat tgctatttta gaaaccttag
     5701 atttcaaagt agaaatgatt ggtgctttta attaaacttt attttctagg ttctacaatt
     5761 taaataaaat aaagagtcaa ccaaggatta aaataggaat ccatccttgg ttgactttct
     5821 tttgtttgta ttgtttttac acgatgctgc ggtatacgtt tcgttaccac tcgtccaagc
     5881 aattgctaat gtaacctttt atttttcgtt ctaatctgca attactattt caatggcagt
     5941 ggttgattta tatcaatacc taaaactata ggcattacat aatacacgac taaaataata
     6001 attattgcac tgattaagtt tacccagaat cctactgatg ccatttgttt gatagatatt
     6061 ttaccagaac caaagataat tgcattcggt ggtgtcccta ctggtaacat gtatgcacag
     6121 ttagccgcca tagctgcagg tgccataagt agtaatggat gcactccaac agcaacagac
     6181 aacgttgcta aaatcggtaa aatcatcgtt gcagttgcag tattagatgt cacttcagtt
     6241 aaaaataaga caaagattgt tatgacaatt acaataagaa tcggactaac accatttaat
     6301 gatttcaact gttcgcctaa ccattttgct aaaccacttt cagaaatacc tttcgctaat
     6361 gctaaaccgc caccaaataa aattaataca ccccaaggga gctcttttgc aacttcccag
     6421 tcaatgatac ggcgatgttt ttcagtattt ttagctggaa taataaataa taatattgat
     6481 ataaaaatag caatcgtacc atctgcaaca gatgacgtaa cttcccattt tttcagaaga
     6541 aactctcttg taatccataa taagctagca agtacaaaga tagtttgtac taccttttct
     6601 tcatacttca ttttgccaag ctcgtctaac ttttgtttaa ttaacgtctg accaccaggt
     6661 aaatatttca aatcatgtct aaacgcaaca tatcttaaat agagccaagt aatacctaac
     6721 aaaacaatga ccgttggaat ccctacaatc atccatttag caaaactaat ttcatatcca
     6781 aaatgttgca tgtattgtcc ttttaaaata attaatggcg gggttccgat taatgtaccc
     6841 aagccaccaa tcgtacctgc atagccaatt gctaaaacta gagatttttc aaacttttga
     6901 atacttgttt gattcgtatt ggcttcttgt aaatcatgtg cttccttaat aattgctaaa
     6961 ccaatcggaa tcataatcat tacagctgca gtgttcgata caaacataga taagaatcct
     7021 gttgccacca tgaatccaag taatattttt gaagtgcttg caccaatcaa attaataatt
     7081 gtcaacgcta ctctcgtatg taaattccat ctttccattg caattgccaa aataaatcca
     7141 cctaaaaaca aaaagataat atcattgcca tattcggatg atacttgttc tggtgtaagt
     7201 atatgaccta atggtaataa cacaattggt aataagctcg ttgctgcaat aggaattgct
     7261 tcagtaatcc accaagtcgc aatccataaa gtaatcgcta aaacatagac gcctttccaa
     7321 ggtaagtctt gtggatgaaa gaataataat gttaatagga aaagtaatgg acctaatatt
     7381 agtccaataa gttgtcccgc actaaacggt ttctttttct ttgattttga aaagaatgtt
     7441 aaataccttt tcgatatcat tgattgatgt tgattgaaat aagccataaa tttccccctc
     7501 atgatgatta tgacaagcta cttcaactca ctatattaga gtatagaaag ttgtctatct
     7561 tgtattatca ttaccgtcaa aatacccctt ttatcagata attgggttta ttatagcata
     7621 tttataccgt tttatgaatg cgctttcaaa aattatctac tattcttaat cctaatgtca
     7681 tgcgaaactt tttacttttt tatttcatct acattgattg tagtaacttg aatacccgct
     7741 tcaacaggac ttaaaaacac tgtatcaata tcttctaaat gaattgattg agtcgaatta
     7801 ttgaatgtat tcccaacgat taatacatct ttatgtttaa cattattata attacgtaca
     7861 tgtatcgcat ctttagacat tgatccttta aattcatttc caattatatt catatttatg
     7921 cctgcttggg aacctaagtc ttttcctgtc atcacatctg ctgcattttt accatctcta
     7981 actcctaaat atctaatgcc accctcacag ttaataaact tattattaat aatgaaagca
     8041 tctttatact tcaagggagt taatgcataa ccttgtatat cttcaaatat attatttcta
     8101 atatgaatat tctcatagta tcgattatgt ctacttgcat gtgagccaat agcacgattc
     8161 caacttccca tttcgggcaa ttctgaaggt ccaaaataac aatcttcgat aatgacattt
     8221 ttcgttatcg taccatctgt cgttccgaat tttggaaaag caccaggtac ttgaatgtct
     8281 aattgtattg cttcagaata aaagcgttcg ccactatagt ctatgaatcc ttcaaatgaa
     8341 cagcttttaa tatagagtcc gttaatccca caagcatcaa ttgcatgacc actcactaca
     8401 ttcttaatgg tcacacctat taattgaata tcttcagcat gcccaatgca catagctgta
     8461 ttgttataag gatacgatac accattcata tcaaacttgc cgcctttaat atgaatatga
     8521 ctgtgtccat tataaccacg ataaaaacca aagcgacgac cattttttaa taaaggacca
     8581 gaatgacatc ggcgtaaaat agtttcatta tctaacaaaa gtgttgtatt gccatatatc
     8641 gttaatggtt tgcaaatatc atacgtccct ttcggtatat aaactgtcgt tggtttacat
     8701 cttccacgat ttaacgcacg ttgtattgct cgagtatctt tagttttgtt tatgcctgtt
     8761 aacccataat ctttcatatt gatatacata aatcgtcatc ctttgtctat tttctaatta
     8821 ctcatatatt aaccaattat tcaaattata atcgtgttag gttgatttaa cagatagtca
     8881 taaatctcgt gatgccatct attagcattt ttatattcaa aacatcaaca cttgatatta
     8941 gttctaagtt tgtcacattt agtattctat tacagctatt ttaaaactat cgcaaaaact
     9001 tcttatatta tttgttgatt gcgtgatttg ctcttccaaa aaagttgatt aatctatata
     9061 cttaatctat atttatatat taaccattag ggttaaaaat tactctagca tttatgaata
     9121 gatgggagtt tattttatta ttatatagga gagatgttga atgacacatc gcgcactatt
     9181 agttgttgac tattcatatg actttatcgc agacgacggc ttactaacat gcggtaaacc
     9241 tggacaaaat attgaagatt ttattgtttc tcgtatcaat gactttaatt attatcaaga
     9301 ccatatattc tttttaatgg atttacatta tttacatgac attcatcatc ctgaaagtaa
     9361 attattccca ccacacaata tcgtagatac aagtggtaga gaattatacg gtaaagtagg
     9421 taaattatac gaaacaatta aagcgcaacc taatgtacat ttcattgata aaacgcgcta
     9481 tgattcgttc tttggtaccc cgcttgatag tttattgaga gaaagaagta ttaatcaagt
     9541 cgaaatcgtt ggtgtatgta ccgatatttg cgtgttacat acagcaattt ctgcatacaa
     9601 cttaggttat aaaatttcag tacctgctga gggagtggct tcatttaatc aaaaagggca
     9661 tgaatgggca cttgcacatt tcaaaaactc attaggtgca gaggtagaac aacacgttta
     9721 aatcgtgcta aaataattat aaagaataca atttacaagg gagatatttg acaatggcta
     9781 aaacatatat tttcggacat aagaatccag acactgatgc aatttcatct gcgattatta
     9841 tggcagaatt tgaacaactt cgaggtaatt caggagccaa agcataccgt ttaggtgatg
     9901 tgagtgcaga aactcaattc gcgttagata catttaatgt acctgctccg gaattattaa
     9961 cagatgattt agatggtcaa gatgttatct tagttgatca taacgaattc caacaaagtt
    10021 ctgatacgat tgcctctgct acaattaagc atgtaattga tcatcacaga attgcaaatt
    10081 tcgaaactgc tggtccttta tgttatcgtg ctgaaccagt tggttgtaca gctacaattt
    10141 tatacaaaat gtttagagaa cgtggctttg aaattaaacc tgaaattgcc ggtttaatgt
    10201 tatcagcaat tatctcagat agcttacttt tcaaatcacc aacatgtaca caacaagatg
    10261 ttaaagcagc tgaagaatta aaagatattg ctaaagttga tattcaaaag tacggcttag
    10321 atatgttaaa agcaggtgct tcaacaactg ataaatcagt tgaattctta ttaaacatgg
    10381 atgctaaatc atttactatg ggtgactatg tgactcgtat tgcacaagtt aatgctgttg
    10441 accttgacga agtgttaaat cgtaaagaag atttagaaaa agaaatgtta gctgtaagtg
    10501 cacaagaaaa atatgactta tttgtacttg ttgttactga catcattaat agtgattcta
    10561 aaattttagt tgtaggtgct gaaaaagata aagttggcga agcattcaat gttcaattag
    10621 aagatgacat ggccttctta tctggtgtcg tttctcgaaa aaaacaaatc gtacctcaaa
    10681 tcactgaagc attaacaaaa taatactata ttactgtcta attatagaca tgttgtattt
    10741 aactaacagt tcattaaagt agaatttatt tcactttcaa tgaactgttt tttatttacg
    10801 tttgactaat ttacaaccct ttttcaatag tagtttttat tcctttagct accctaaccc
    10861 acagattagt gatttctata caattcccct tttgtcttta cattttctta aaatatttgc
    10921 gatgttgagt ataaattttt gttttcttcc tacctttttc gttatgatta aagttataaa
    10981 tattattatg tacacgattc atcgctctat tttcaacttt caacatatat aattcgaaag
    11041 accatttaaa attaacggcc acaacattca aatcaattaa tcgctttttc caaaataatc
    11101 atataaggag gttcttttca ttatgaatat cattgagcaa aaattttatg acagtaaagc
    11161 ttttttcaat acacaacaaa ctaaagatat tagttttaga aaagagcaat taaagaagtt
    11221 aagcaaagct attaaatcat acgagagcga tattttagaa gcactatata cagatttagg
    11281 aaaaaataaa gtcgaagctt atgctactga aattggcata actttgaaaa gtatcaaaat
    11341 tgcccgtaag gaacttaaaa actggactaa aacaaaaaat gtagacacac ctttatattt
    11401 atttccaaca aaaagctata tcaaaaaaga accttatgga acagttttga tcattgcacc
    11461 atttaactat ccttttcaac tagtgttcga acctttaatc ggtgctattg cagcaggtaa
    11521 tacggcaatt attaaaccat ctgagttgac gcctaatgtt gcacgagtga ttaaacggtt
    11581 aatcaatgaa acatttgatg caaattacat tgaagttatt gagggaggaa ttgaagaaac
    11641 gcaaacgtta attcacttac cttttgacta tgtcttcttt acaggaagtg aaaatgtagg
    11701 caaaatcgtt tatcaagctg ccagcgaaaa tttagttcct gtgacattag aaatgggcgg
    11761 taaatctcca gtcatcgttg atgaaacagc gaatattaaa gttgctagtg agcgcatttg
    11821 ttttgggaaa ttcactaatg ccggccaaac atgtgttgca ccagattaca ttttagtaca
    11881 cgaatctgta aaagatgatt taatcacagc cctatcaaaa acgttgcgtg aattttatgg
    11941 tcaaaatata caacaaagtc cagattatgg ccgcattgta aaccttaaac attatcatcg
    12001 tctgacttca ttacttaaca gtgcacaaat gaatattgta tttggtggtc atagtgatga
    12061 ggatgaacgt tatatagaac caacattgtt agatcacgtt acaagtgatt cagcaattat
    12121 gcaagaagaa atttttggtc ctatcttacc gattttaacg tatcagtcat tggatgaagc
    12181 aatagccttt attcaccaaa gaccaaaacc tttgagttta tatttattta gcgaagatga
    12241 aaatgctaca caacgtgtaa taaacgagct atcatttggc ggcggcgcta ttaatgatac
    12301 attgatgcac ctagcgaatc ctaaattacc atttggtggt gttggcgcct caggtatggg
    12361 acgctatcat ggtaaatatt cattcgacac ttttacacat gaaaaaagct acattttcaa
    12421 atctacacga ttagaatcag gtgtccattt accaccatat aaaggtaaat ttaaatacat
    12481 caaagctttc tttaaaaatt aatttttgag acaaaaataa accatgcttc cataatttga
    12541 tgttcttgaa aacaataaat ttcaaggtat acacaaattt tttggaaaca tggttttctt
    12601 tttatcatga ttaattaaag attaaaatat gacacataca ccttcagatg ctacacgagt
    12661 gttatcacat agcgtaattt tacctgtttc tttatttctt tcgaaaacag ttacaactga
    12721 atctccttgc tcatgagcac aaactaaaag gtcatctgat gaggcaatat taaaatctct
    12781 tgggaattga ccaccacttt cagtaattgt tactagttct aagtgttgac cattatcaag
    12841 aactttaaaa attgcaatgc tatcatgccc tctattagat acatataaga attgttgatc
    12901 atgagataaa cgcactgctg caagtttagt atctccatca aagttttcag gaattgttaa
    12961 atgacgctcg agctcttcaa atttaccgtc attatattct gcaacactca cagtatttga
    13021 taattcgtgt acgacataag caaattttcc attatcatga aattcaatat gtcttgtccc
    13081 atcactatct ttaaataaag attctttata aaattcaaac ccgttgtcat caaatttata
    13141 agtaacgata cgatcagcac ctaaatctgt tactgcaacg tacttaccat ctggagtttg
    13201 attaatataa tgtgcgtgtg gatgatcttg tctttcatgt gtacctgttg gaaaatcatg
    13261 tgcaagttct tgaattagac gtataatttc acctgtgtgc gtatttaatt catacatacg
    13321 tatgatgcca gcaccatata ccgcttcaaa taaatatcgt ttatcttctg aaatcgatac
    13381 ataacaacct gtaccagctt ttgaagacaa acatttattt aataaatgca attcaccatt
    13441 gtcatcaatt ttcaaacttg caacaccgca ttgttctcct tctttgttga ttccatataa
    13501 aacttcatta ttacgcacca aatatgtaga cgcttctaat tcaaatcctg tttctaataa
    13561 atcaatacgt gactgatttt cgtttaattc aaaacgataa atccctttac cattcttttt
    13621 agtgtaagaa ccaatatatc catttgtcat tacttttacc cctccatgtt agtcttattt
    13681 tcattttatt aaattttagc tagaattaac aatcatttat catttcctta aagctaaaac
    13741 tatttaacta ctttaaaaga actatttgct gtgatatgtc attattcgat tttaattaat
    13801 ccctcttttt tacttacacg agcattaatt attaacgcgt ttatgcttcc acaaaatttt
    13861 tgttgcaaaa tagaacacat gttcgtataa tgtaattatc agattggggt gaattaagga
    13921 tgacaagtta tgaagaagaa actgattatc gtaacgttcc aagagaatta ctagataaca
    13981 atatacctat gggaagaggc atgattaaat gggctccttt tgcaacatta cccgaacaat
    14041 tcgaaacgat tcaacaatac attatcgacc aaaacaaaat tacacgtcct gtattatcag
    14101 atgatcaatt ggctgagcta aacattcggc tacacgaggc gctacaatat gcccaacctg
    14161 tagaagttaa attttataat aatggctttg tagattcagt acgcttaacc atttatcgta
    14221 ttgatgctat taattatgaa attgatggtt atgtttacaa tcagcaacaa cgtcaaaaaa
    14281 tttctatctt tgatatctta gatattgtct tgttacctta atgctttgaa cataattaaa
    14341 cagattgagt atatgcatat aagatgattt ttaacatcat ttttggatga taggatgttg
    14401 cgccacgatg atgtctgaat tcatcgaatt cgctatcagg tattgtttca acaatttcat
    14461 taacatatcg tgaaatatca ttttgaggga ttctaacaga agtttctatt ggtagtgtag
    14521 ttggggcatg ttataatttt tatacataag gcacctcgtt aatttagttt agtggtattt
    14581 attaaattat aagaagggac ccaacacaga aaattcattt tattgaattt tacatttatg
    14641 tgcaagttgg gcaaagtgtc ttatttttta aaagtatttc aaagtaaaat tacatgttaa
    14701 tacgtagtat taatggcgag actcctgagg gagcagtgcc agtcgaagac caaggctgag
    14761 acggcaccct aggaaagcga agccattcaa tacgaagtat tgtataaata gagaacagca
    14821 gcaagatatt ttctaattga aaatcatctt actgctgttt tttaggaatc gatggggttt
    14881 aaaaattgat gatagttatc aatggatatg tctctggttt gaatcgtctt accgaatgaa
    14941 tacgaaatat ctataatatt ttcaatttcg agttcggcag ttttgtcttg agcaatgtca
    15001 taaatgactg catgccaatg tccatgttta cacaaaaggc caataaatat aacgttttca
    15061 gctttaatat tatcatcaac cctatgatct actaacatca catttcgttc tgtacaataa
    15121 attaaaatgt cggaaaacag cattgggagt gtcatatgtt gtgtgtcatc aaatttaata
    15181 taaaagtcca tgcgtttaag caattttctt aagcgtgttt gtggaagact taaacattgt
    15241 tttataattg cgttgatttc gcttttatat ggtaatgtcg agtacgactg actttcatta
    15301 agcactaaaa ataaggctga taattgtgtt tcagataagt ttaatgcaat tttagattgt
    15361 gcttgttgta tttggtaacc accatttttc ccataatgcg cataaatttt aacaccttga
    15421 ttttctaaat catcaatatc tcttaaaatt gtgcgtttgg atacgttgca atatttagct
    15481 aattctaaag cggtcatttt tttattttgt tgaattgcag taattattaa attttgcctt
    15541 tcagctttat tcatgacagc cacctcctaa aagatgatat attacacaaa gtataacata
    15601 agagtgacaa tgaggacatt ttcaatatct tttaaaaaaa atcgtaaaaa ttttgtggaa
    15661 aacgcttaca ttttaaaaaa gattgtgcta tgatattact tgtaacaggg ggagcgatta
    15721 aacaaagggg tagagctata taacagatag cttttaatcg ttcaagttac atcacaacat
    15781 cttcaatatt tattacttac tttcctttct atttgtcggc tagcacgtga ctagccgatt
    15841 tttttatgca taaaatgaag aagtcttcag ttgtattgtt gattctttta atatagggta
    15901 ataataaaat gataatgata tgagaagtgg tggacgaaat gacagataaa acattacagc
    15961 aaatagataa gttaatatgt tcgtggttga aacaaataga caatgttatt ccgcaattaa
    16021 ttatggaaat gactacagaa actaagcgtc atcgatttga tttagttaca aatgtagata
    16081 aacagattca acaacaattc caacaatttt tagcaacaca ttttccggaa caccaactat
    16141 tagcggaaga aaaaagtaat gaaatgatta cgaatgaaat taatcattta tggattatgg
    16201 atcccattga tggaactgcc aatttagtaa aacagcaaga agattattgt attatattgg
    16261 cgtattttta tgaaggtaaa ccaatgttat cgtacgtata tgattatccg cataaaaagc
    16321 tttataaagc aatacgagga gaaggtgctt tttgtaatgg aattaagatg gaagaaccac
    16381 catcgttaaa attggaagac gctattatat cgtttaatgc acaagtgatg aatctagata
    16441 cggtgcaaga tttatttgac gcctcattta gttatcgttt agttggtgct tgcggattag
    16501 attctatgcg agtggcaaaa ggtcaatttg gggcacatat taatacaaat cctaaacctt
    16561 gggacattgc tgcacagttt ttatttgcag aactattgaa tctaaaaatg acgactttag
    16621 atggtaaggc aattgatcat ttaaagggtg caccttttat tataagtaat aaagcatgtc
    16681 atgaaacggt acttaaaatt ttaaatgcaa atggtggtta tcaaaaatac agatagatat
    16741 tctctagaga taaaaaatga taaaaaatag ttatacgtct acagaagtat agtaaaaaaa
    16801 ttattttact atataataaa tagagaaaac agacaaagta tgtaaggaat agtaaaggag
    16861 tggtgggatt gaaacgttcg tctaaaagta aaatgagcct accgaaaaaa atatttttat
    16921 gggtattcgg tattttagta atcttagcga ttgtagctgt tgtgtatgta gctgctaaaa
    16981 tttttattac tggtaataag atacacaatc cgttagatcg caatcattca gaattaagag
    17041 ataaaaaagt gagtttaaac gatggtgatc cattcacaat cgctttattt ggtgtcgatt
    17101 cagatgctga tcgtaagaaa aagggtggcg gagagcgtag tgatagtatt atgattttat
    17161 ctatcaaccc taaaacgaag aaaactgaaa tagtaagtat accgcgtgat acaagagcgg
    17221 aaattgttgg ccgtggtact actgaaaaaa ttgcacatgc ctatgcatat ggtggaccaa
    17281 atatggctgt taaatcactt gaaaaattaa tgaatgtgcc aattgatcat tatgcgacaa
    17341 tcgatatgga tggtttgcat aatatgatag atagcatcgg cggtgttgat gtagtaagta
    17401 atgatacatt cactgttgat ggtgttcgtt ttacaaaagg acagcagaca catgtaaatg
    17461 gtgatcaagc attgaagttt attagaagtc gtaaagaaga aggtgctggt ggtgactttg
    17521 gtcgacaaca acgtcagcaa attgtgcttg aagcgatggc caataagatt gcaagcccat
    17581 catcgattac acattttaat agtctaatga atgaaattca aaataatgtt aaaacagatt
    17641 taacattagg tgatttgaat acgattagaa gcaattataa agatgctaat gacacgatta
    17701 ataaacatca attgagtggt caaggcggca ttcaaagtga cggattgtac tacttcattc
    17761 ctagtgagca gtcaaaagct gaaagtacta agttattaaa agataatct
//
LOCUS       DAKTIO010000042        17691 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.042, whole genome shotgun sequence.
ACCESSION   DAKTIO010000042 DAKTIO010000000
VERSION     DAKTIO010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17691
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.042"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(74..1351)
                     /locus_tag="O0293_001057"
     CDS             complement(74..1351)
                     /locus_tag="O0293_001057"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HCY0279949.1"
                     /translation="MQNLDKNFRHLSRKEKLQQLVDKQWLSEEQFDILLNHPLIDEEV
                     ANSLIENVIAQGALPVGLLPNIIVDDKAYVVPMMVEEPSVVAAASYGAKLVNQTGGFK
                     TVSSERIMIGQIVFDGVDDTEKLSADIKALEKQIHKIADEAYPSIKARGGGYQRIAID
                     TFPEQQLLSLKVFVDTKDAMGANMLNTILEAITAFLKNEFPQSDILMSILSNHATASV
                     VKVQGEIDVKDLARGERTGEEVAKRMERASVLAQVDIHRAATHNKGVMNGIHAVVLAT
                     GNDTRGAEASAHAYASRDGQYRGIATWRYDQDRQRLIGTIEVPMTLAIVGGGTKVLPI
                     AKASLELLNVESAQELGHVVAAVGLAQNFAACRALVSEGIQQGHMSLQYKSLAIVVGA
                     KGDEIAKVAEALKKEPRANTQAAEHILQEIRQQ"
     gene            1605..2771
                     /locus_tag="O0293_001058"
     CDS             1605..2771
                     /locus_tag="O0293_001058"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HCY0279950.1"
                     /translation="MTIGIDKINFYVPKYYVDMAKLAEARQVDPNKFLIGIGQTEMAV
                     SPVNQDIVSMGANAAKDIITDEDKKKIGMVIVATESAVDAAKAAAVQIHNLLGIQPFA
                     RCFEMKEACYAATPAIQLAKDYLATRPNEKVLVIATDTARYGLNSGGEPTQGAGAVAM
                     VIAHNPSILALNEDAVAYTEDVYDFWRPTGHKYPLVDGALSKDAYIRSFQQSWNEYAK
                     RQGKSLADFASLCFHVPFTKMGKKALESIIDNADETTQERLRSGYEDAVDYNRYVGNI
                     YTGSLYLSLISLLENRDLQAGETIGLFSYGSGSVGEFYSATLVEGYKDHLDQAAHKAL
                     LNNRTEVSVDAYETFFKRFDDVEFDEEQDAVHEDRHIFYLSNIENNVREYHRPE"
     gene            complement(2941..3462)
                     /locus_tag="O0293_001059"
     CDS             complement(2941..3462)
                     /locus_tag="O0293_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCY0279951.1"
                     /translation="MEYKSYYDSPVGRLELLSDGVSLTAVLFENQQGDGTREENTSLA
                     IFREATQWLDAYFKGDNPEITIPLKPTGSHFQQCVWNELRQVPYGTLTTYGAIAKKVG
                     KLLNKPKMSAQAVGGAVGSNPLSIIVPCHRVVGKTGSLTGFGGTINNKIKLLELENID
                     MSKLYVPKHSTKP"
     gene            3871..5976
                     /gene="clpL"
                     /locus_tag="O0293_001060"
     CDS             3871..5976
                     /gene="clpL"
                     /locus_tag="O0293_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpL"
                     /protein_id="HCY0279952.1"
                     /translation="MNNGFFNSDFDSIFRRMMKDMQGSNQVGNKKYYINGKEVSPEEL
                     AQLTQQGGNHSAEQSAQAFQQAAQRQQGQQGGNGNYLEQIGRNLTQEARDGLLDPVIG
                     RDKEIQETAEVLSRRTKNNPILVGEAGVGKTAIVEGLAQAIVEGNVPAAIKDKEIISV
                     DISSLEAGTQYRGAFEENIQKLIEGVKSSQNAVLFFDEIHQIIGSGATGSDSGSKGLS
                     DILKPALSRGEISIIGATTQDEYRNNILKDAALTRRFNEVLVNEPSAKDTVEILKGIR
                     EKFEEHHQVKLPDDVLKACVDLSIQYIPQRLLPDKAIDVLDITAAHLSAQSPAVDKVE
                     TEKRISELENDKRKAVSAEEYKKADDIQNEIKSLQDKLENSNGEHTAVATVHDISDTI
                     QRLTGIPVSQMDDNDIERLKNISNRLRSKIIGQDQAVEMVSRAIRRNRAGFDDGNRPI
                     GSFLFVGPTGVGKTELAKQLAIDLFGNKDALIRLDMSEYSDTTAVSKMIGTTAGYVGY
                     DDNSNTLTEKVRRNPYSVILFDEIEKANPQILTLLLQVMDDGNLTDGQGNVINFKNTI
                     IICTSNAGFGNGNDAEEKDIMHEMKKFFRPEFLNRFNGIVEFLHLDKDALQDIVNLLL
                     DDVQVTLDKKGITMDVSQDAKDWLIEEGYDEELGARPLRRIVEQQVRDKITDYYLDHT
                     DVKHVDIDVEDNELVVKGK"
     gene            complement(6045..6215)
                     /locus_tag="O0293_001061"
     CDS             complement(6045..6215)
                     /locus_tag="O0293_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HCY0279953.1"
                     /translation="MSVIINILIFLAIFGYALYTLVKFFKRSKQGKCGTCDINRDCCG
                     TEQHTANHFPGK"
     gene            complement(6228..8222)
                     /gene="feoB"
                     /locus_tag="O0293_001062"
     CDS             complement(6228..8222)
                     /gene="feoB"
                     /locus_tag="O0293_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCY0279954.1"
                     /translation="MENYCILGNPNVGKTSLFNALTGSYEYIGNWSGVTVEKKVGKLK
                     ENVGQLIDLPGTYDLSPISKDETVVTDYLLNDSFSGIINIVDASQLKRNMQLTVQLLE
                     LNQPIYIGLNMIDVATKRGIKIDYHKLMKKLKTPIFPVVARTGKGTKYLLGEIKHLGE
                     GYQPHFKINYGEKIEETIKNMCQIIMTETSHDKYQARFIAIQFLLNNMQIANELNSEV
                     VNKLSSLRDQVAEQVGAVSVRREMERIRNHYIETLLQDVVTYPDEDKQYFSSRIDKIL
                     THKYIGMPIFLAIMWLIFQTTFTWIGTPLSDQLDAFIGGTFTDSVKTIMNYLGVIPFL
                     QDLITDGIIAGVGSVLVFVPQIVVLFFFISLLEDSGYMARIAVLMDRIMESFGLSGKS
                     FIPMIIGFGCNVPSIMAARSIENEKERLTTILIAPFMSCSARLPVYALFVGIFFKENQ
                     SLVVLSLYVLGIIMAFLVSTVLTKTILKNDNAIFIVELPTYRVPSIKTLWRSTWEKAK
                     GFVRKAGTFIFGGSVVIWLLSYVGPHGINVNINQSFLHMVGSFFGMLVQPLGFGTWQA
                     GATLVPGFLAKEVIVSSMAIIYSSGDAGLVNVIQNQFTPLSAYAFMIFILLYIPCVST
                     VAAIRKETYSWKWTALAVAYPLVTAYVLTFIFYQVGHLFV"
     gene            complement(8234..8461)
                     /locus_tag="O0293_001063"
     CDS             complement(8234..8461)
                     /locus_tag="O0293_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCY0279955.1"
                     /translation="MLNIKNGEMNKAYKIKRMDIANENMLYRLSAFGLTDDAIITIKQ
                     KCLFKGPCIIEVNGQQLSIRHCDACSIALEE"
     gene            complement(8677..11166)
                     /gene="farE"
                     /locus_tag="O0293_001064"
     CDS             complement(8677..11166)
                     /gene="farE"
                     /locus_tag="O0293_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux MMPL transporter FarE"
                     /protein_id="HCY0279956.1"
                     /translation="MAKFLYKMGTFIAKHKWSAVIAWIVIVAAILIPLATNAPKFDND
                     IKMTGLESLDTNKKIEKHFNQDSEKAQIRVVFKTTKDDGIVQPNITKDIKKTLDDIKK
                     DDKHIDKISDPYENKQISKDKTTAFADITYDVSQTSLKDGSRDNVKSHLKDLRDNHNV
                     QTELTGTGMTSTEVGGNSELVGIIVAFVVLLITFGSVIAAGLPIISALIGLASGVGII
                     SLLTYAFDIPNVTLTLAVMIGLAVGIDYALFILFRYRQVMKTETDYIKGIGLAIGTAG
                     SAVVFAGVTVVIAVCGLSLVGIDFLAVMGFASAISVVFAVLSALTLLPALISIFHKRI
                     KVNKLQSKFKKDIDTPWSKFITGNALAAVLLGLIILVAAAIPVSHMRLGIPDDGVKPA
                     DSTQKKAYDIISDKFGEGFNGQIPMLINVKDKKDDPQGLQQDLQSVYKDIKDKKNVDI
                     VTPPQMSKDNDYALMVVIPKQGPNAESTNDLVHDLRDYHKDAQDKYGFKTEISGQSVI
                     NIDMSKKLNEAIPLFASVIVVLAFFLLMIVFRSILIPLKAVLGFVLSLMATLGFTTLV
                     MQDGFMKGLFGIETTGPMLAFLPVITIGILFGLAMDYEVFLMSRIHEEYSKTGDNDYS
                     IKVGLKESGPVIVAAALIMFSVFFAFVFQEDVMIKSMGMALAFGVLFDAFVVRMMLIP
                     ALTKLFGKGSWYLPAWLNRIIPRVDIEGHALEKYKTVESQESEAKDSKETYDTTFKVY
                     PQGATNVSKHQDAHGQDDAHSIVLDDKTMALYQEVKQQSASSLFLYDALIDYQNKHQL
                     NSKKQATNIEQLNKNIEKLNQLLEKNLRNKS"
     gene            11311..11859
                     /gene="farR"
                     /locus_tag="O0293_001065"
     CDS             11311..11859
                     /gene="farR"
                     /locus_tag="O0293_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux pump transcriptional regulator
                     FarR"
                     /protein_id="HCY0279957.1"
                     /translation="MKETDLRVIKTKKALSSSLLQLLEQQLFQTITVNQICDNALVHR
                     TTFYKHFYDKYDLLEYLFNQLTKDYFARDISDRLNHPFQTMSDTINNKEDLREIAEFQ
                     EEDAEFNKVLKNVCIKIMHNDIKNNRDRIDIDSDIPDNLIFYIYDSLIEGFIHWIKDE
                     KIDWPGEDIDNIFHRLINIKIK"
     gene            complement(11936..12103)
                     /locus_tag="O0293_001066"
     CDS             complement(11936..12103)
                     /locus_tag="O0293_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279958.1"
                     /translation="MRYVISIIMGIVLAIWSFKQLSQSHLDSGFIFFFIVYVLCISCF
                     NSDKHDKNKKR"
     gene            complement(12215..13759)
                     /gene="pruA"
                     /locus_tag="O0293_001067"
     CDS             complement(12215..13759)
                     /gene="pruA"
                     /locus_tag="O0293_001067"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HCY0279959.1"
                     /translation="MVVEFKNEPGYDFSVQENVDMFKKALKDVEKELGQDIPLVINGE
                     KIFKDDKIKSINPADTSQVIANASKATKQDVEDAFKAANEAYKSWKTWSANDRAELML
                     RVSAIIRRRKAEIAAIMVYEAGKPWDEAVGDAAEGIDFIEYYARSMMDLAQGKPVLDR
                     EGEHNKYFYKSIGTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPYIAYKLM
                     EILEEAGLPKGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEG
                     QNFLKRVIAEMGGKDAIVVDENIDTDMAAEAIVTSAFGFSGQKCSACSRAIVHKDVYD
                     EVLEKSIKLTKELTLGNTVDNTYMGPVINKKQFDKIKNYIEIGKEEGKLEQGGGTDDS
                     KGYFVEPTIISGLKSKDRIMQEEIFGPVVGFVKVNDFDEAIEVANDTDYGLTGAVITN
                     NREHWIKAVNEFDVGNLYLNRGCTSAVVGYHPFGGFKMSGTDAKTGSPDYLLHFLEQK
                     VVSEMF"
     gene            complement(13949..14548)
                     /locus_tag="O0293_001068"
     CDS             complement(13949..14548)
                     /locus_tag="O0293_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCY0279960.1"
                     /translation="MTEKEKMLAEKWYDANFDQYLINERARAKDICFELNHTRPSATN
                     KRKELIDQLFQTTTDNVSISIPFDTDYGWNVKLGKNVYVNTNCYFMDGGQITIGDNVF
                     IGPNCGFYTATHPLNFHHRNEGFEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAG
                     SVVTKDIPPHSLAVGNPCKVVRKIDNDLPSETLNDETIK"
     gene            14787..14978
                     /gene="cwrA"
                     /locus_tag="O0293_001069"
     CDS             14787..14978
                     /gene="cwrA"
                     /locus_tag="O0293_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall inhibition responsive protein CwrA"
                     /protein_id="HCY0279961.1"
                     /translation="MRILITGTVAILIILGLVKTIQDYEMTNDTSRQLSDNKDDDKVI
                     HLNNFKNLHAKEFNPSDFF"
     gene            15218..17626
                     /locus_tag="O0293_001070"
     CDS             15218..17626
                     /locus_tag="O0293_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCY0279962.1"
                     /translation="MANTKKTTLDITGMTCAACSNRIEKKLNKLDDVNAQVNLTTEKA
                     TVEYNPDQHDVQEFINTIQHLGYGVAVETVELDITGMTCAACSSRIEKVLNKMDGVQN
                     ATVNLTTEQAKVDYYPEETDADKLVTRIQKLGYDASIKDNNKDQTSRKAEALQHKLIK
                     LIISAVLSLPLLMLMFVHLFNMHIPALFTNPWFQFILATPVQFIIGWQFYVGAYKNLR
                     NGGANMDVLVAVGTSAAYFYSIYEMVRWLNGSTTQPHLYFETSAVLITLILFGKYLEA
                     RAKSQTTNALGELLSLQAKEARILKDGNEVMIPLNEVHVGDTLIVKPGEKIPVDGKII
                     KGMTAIDESMLTGESIPVEKNVDDTVIGSTMNKNGTITMTATKVGGDTALANIIKVVE
                     EAQSSKAPIQRLADIISGYFVPIVVGIALLTFIVWITLVTPGTFEPALVASISVLVIA
                     CPCALGLATPTSIMVGTGRAAENGILFKGGEFVERTHQIDTIVLDKTGTITNGRPVVT
                     DYHGDNQTLQLLATAEKDSEHPLAEAIVNYAKEKQLILTETTTFKAVPGHGIEATIDH
                     HYILVGNRKLMADNDISLPKHISDDLTHYERDGKTAMLIAVNYSLTGIIAVADTVKDH
                     AKDAIKQLHDMGIEVAMLTGDNKNTAQAIAKQVGIDTVIADILPEEKAAQIAKLQQQG
                     KKVAMVGDGVNDAPALVKADIGIAIGTGTEVAIEAADITILGGDLMLIPKAIYASKAT
                     IRNIRQNLFWAFGYNIAGIPIAALGLLAPWVAGAAMALSSVSVVTNALRLKKMRLEPR
                     RKDA"
BASE COUNT         5779 a         3320 c         2826 g         5766 t
ORIGIN      
        1 gttcatatta atttatctaa gcgctacgat ggactgactt aaacttttct ttaaaatcgc
       61 tattcgccat ttactattgt tgtctaattt cttgtaaaat atgttccgct gcttgtgtat
      121 ttgcacgggg ttcttttttc aaagcttcag ctactttagc aatttcatca ccttttgccc
      181 ctacaacgat agctaatgat ttatattgta aactcatatg accttgttga ataccttctg
      241 acacaagcgc gcgacatgct gcaaagtttt gcgctaaacc aacggcagca actacatgac
      301 ctaattcttg tgctgactct acatttagta gctctaatga agctttagca attggtaata
      361 cttttgtacc accgccaacg attgccaatg tcataggcac ttcaattgta ccaatcaatc
      421 gttgacgatc ttgatcgtaa cgccatgtag caataccacg atactgtccg tcacgactcg
      481 cgtatgcatg cgcacttgct tctgcaccac gcgtatcatt tcctgttgct aaaacaacag
      541 catgtatgcc attcataaca cctttattat gtgttgctgc acgatgaata tctacttggg
      601 ccaatacaga agcacgttcc attcgtttgg caacctcttc tccagttctc tcgccccttg
      661 ctaaatcttt aacatcaatt tcgccttgaa ctttaacaac ggacgctgtt gcatgattgg
      721 ataaaatact cattaaaatg tcgctttgcg gaaattcatt ttttaaaaat gcagttatgg
      781 cctctaaaat cgtattaagc atattagcgc ccatagcatc tttcgtatca acaaatactt
      841 ttaaagatag taactgttgc tcaggaaatg tatcaatcgc tatacgttgg taaccaccac
      901 cacgcgcttt aatagaagga tatgcctcat ccgcaatttt atgaatttgc ttttctaaag
      961 ctttaatgtc tgctgataat ttttcagtat cgtcaacgcc atcaaagacg atttgaccta
     1021 tcataatacg ttcagaagat accgttttaa atccgccagt ctgattcact agctttgcac
     1081 cataactagc tgcagcgaca actgaaggct cttccaccat cataggtaca acatatgcct
     1141 tatcgtccac aatgatattc ggtaataatc caacgggtaa tgcaccttgc gcgatgacat
     1201 tttcaattaa actattggct acttcttcat cgattaatgg atgattcagt aaaatgtcga
     1261 attgttcttc tgataaccat tgcttatcaa ccaattgttg taacttttct ttacgagata
     1321 aatgtcgaaa attcttatct aaattttgca tggacgtact ccttttactt cacataattt
     1381 ttaacatttc aatcactact atttttacca caaaataacg tcattcgtct taaaattcaa
     1441 ttgaataatt gtcgttttga ctttaaaata aaacaaggta aattaaaacg cttacaagaa
     1501 acgacaaatc atttttaaat ttagtatatt tctttgtata aaattagcat attctgatat
     1561 gatacaagtg ttgcttttat aaatttgaaa ggatgtaaaa ccttatgaca ataggtatcg
     1621 ataaaataaa cttttacgtt ccaaagtact atgtagacat ggctaaatta gcagaagcac
     1681 gccaagtaga cccaaacaaa tttttaattg gaattggtca aactgaaatg gctgttagtc
     1741 ctgtaaacca agacatcgtt tcaatgggcg ctaacgctgc taaggacatt ataacagacg
     1801 aagacaaaaa gaaaattggt atggtaattg tggcaactga atcagcagtt gatgctgcta
     1861 aagcagccgc tgttcaaatt cacaacttat taggtattca accttttgca cgctgctttg
     1921 aaatgaaaga agcttgttat gctgcaacac cagcaattca attagctaaa gattatttag
     1981 caactagacc gaatgaaaaa gtattagtta ttgctacaga tacagcacgt tatggattga
     2041 attcaggcgg cgagccaaca caaggtgctg gcgcagttgc gatggttatt gcacataatc
     2101 caagcatttt ggcattaaat gaagatgctg ttgcttacac tgaagacgtt tatgatttct
     2161 ggcgtccaac tggacataaa tatccattag ttgatggtgc attatctaaa gatgcttata
     2221 tccgctcatt ccaacaaagc tggaatgaat acgcaaaacg tcaaggtaag tcgctagctg
     2281 acttcgcatc tctatgcttc catgttccat ttacaaaaat gggtaaaaag gcattagagt
     2341 caatcattga taacgctgat gaaacaactc aagagcgttt acgttcagga tatgaagatg
     2401 ctgtagatta taaccgttat gtcggtaata tttatactgg atcattatat ttaagcctaa
     2461 tatcattact tgaaaatcga gatttacaag ctggtgaaac aatcggttta ttcagttatg
     2521 gctcaggttc agttggtgaa ttttatagtg cgacattagt tgaaggctac aaagatcatt
     2581 tagatcaagc tgcacataaa gcattattaa ataaccgtac tgaagtatct gttgatgcat
     2641 atgaaacatt cttcaaacgt tttgatgacg ttgaatttga cgaagaacaa gatgctgttc
     2701 atgaagatcg tcatattttc tacttatcaa atattgaaaa taacgttcgt gaatatcaca
     2761 gaccagagta gtcggtgtat ttaaaacaca tataataaaa cctaaaagca gcagtaagac
     2821 cacttctaat tgaaatcgtc ttactgctgt tctctattta taacacttcg tattgaatga
     2881 attcattatg cctatttgac acattattga agttttccta atgcctggat cctttatacg
     2941 ttacggcttc gtgctatgtt ttggtacata aagctttgac atatcgatat tctccaactc
     3001 taacagctta attttattat taatcgttcc accgaaccct gttaagctac ccgttttacc
     3061 gacaacacga tgacatggca cgataataga taatggatta cttccgactg cacctccaac
     3121 cgcttgggct gacatttttg gcttgttaag cagcttgcct acttttttgg caatagcacc
     3181 atacgttgtt agagtcccat aaggaacctg tcttaattca ttccaaacac actgttgaaa
     3241 atgactacct gttggcttta aaggtattgt gatttcagga ttgtcacctt taaaatacgc
     3301 gtctaaccac tgtgtcgcct ctctaaatat cgctaaagac gtattttctt ccctagtacc
     3361 atcaccttgt tgattttcaa acaaaacagc ggtcagactt accccatcac tcaaaagttc
     3421 caatcgtcct acaggcgaat catagtaact cttatactcc ataaaaatcc cccctttttc
     3481 taatcatacg ccttaattgt taaattagaa aatattcatg ttcacatttt ttaaaaataa
     3541 ataagtcgtc aattacgtat ataaacacgt aataccagct atcactttgc tgcaatatac
     3601 agttacatat cttactacac gtgctaacct cttactttgt aaaccaaatc ttaaattaaa
     3661 atattgaaaa tgcaatgaat ccttaatatt ttattaaacc tataattact tattaaaaat
     3721 aacacacaat attcataaag ttttaaaaat attctgtttt atcacctact attagtggaa
     3781 aagtacaatt gcaattgtat atagtttgca taacgcttca aaagtaattt cttttttgtt
     3841 tagttcaaaa aaatttagag gtgatgttat atgaataacg gttttttcaa tagcgacttt
     3901 gattcaattt ttcgaagaat gatgaaagat atgcaaggtt caaatcaagt cggaaacaaa
     3961 aagtactata ttaatggtaa agaagtttca cctgaagaac tagcgcaact cacacaacaa
     4021 ggtggcaatc actctgctga acaaagtgcg caagcttttc aacaagcagc acaaagacaa
     4081 caagggcaac aaggtggcaa cggcaattat ttagaacaaa ttggtcgtaa ccttacgcaa
     4141 gaagcacgtg acggtttatt agatccagtc attggtcgtg ataaagaaat tcaagaaact
     4201 gctgaagttt taagtagacg aactaaaaac aatcctatat tagttggaga agctggtgtt
     4261 ggtaaaactg cgattgttga aggtttagca caggcaatcg ttgaaggaaa tgtaccagca
     4321 gcaatcaaag acaaagaaat tatttctgta gacatttcat cattagaagc tggaacgcaa
     4381 tatcgtggtg cttttgaaga aaatattcaa aaattaatcg aaggtgttaa atcttcacaa
     4441 aatgccgtac tattctttga tgaaatccat caaattatcg gttcaggtgc cacaggaagt
     4501 gattcaggta gcaaagggtt atctgatatt ttgaaacctg cattaagtcg tggtgagatt
     4561 tctattattg gtgcaacaac acaagatgaa tatcgaaaca atattcttaa agatgctgca
     4621 ttaacgcgca gatttaatga agtgcttgtt aatgaaccaa gcgctaaaga tactgttgaa
     4681 attttaaaag gtattcgcga aaaattcgaa gaacaccatc aagtaaaatt accagatgac
     4741 gtattaaaag catgtgttga cttatcaatt caatatattc cacaacgatt attaccagat
     4801 aaagcaatcg atgtgttaga tattacagca gcacatttat ctgcgcaaag tccagctgtc
     4861 gataaagttg aaactgaaaa acgaatttct gaattagaaa atgataaacg taaagcagta
     4921 agtgctgaag aatataaaaa agctgacgac attcaaaatg aaatcaaatc attacaagat
     4981 aaattagaaa atagtaatgg tgaacatact gctgttgcta cagttcatga tatttcagat
     5041 actattcaac gattaactgg tattccagtt tctcaaatgg atgataacga tattgaacgt
     5101 ttaaaaaata tttctaatcg tttaagaagt aaaatcatag gtcaagatca agctgtagaa
     5161 atggtttcac gtgcaattcg ccgtaatcgt gctgggtttg atgacggcaa ccgtccaatt
     5221 ggcagtttcc tatttgttgg ccctactggt gttggtaaaa cagagcttgc taaacaatta
     5281 gcaattgatt tatttggtaa taaagatgca ctgattcgac ttgatatgag tgaatatagt
     5341 gacacaacag ctgtttcaaa aatgattggt acaactgctg gttatgttgg ttatgatgac
     5401 aattcaaata cgttaactga aaaagtacgc cgtaatccat actcagtcat tctatttgat
     5461 gaaatcgaaa aagcaaatcc acaaatttta acattgttat tacaagtaat ggatgatggt
     5521 aatttgactg atggtcaagg taatgtcatc aactttaaaa atacaattat tatttgtaca
     5581 tcaaatgctg gctttggcaa tggcaatgac gctgaagaaa aagatattat gcacgaaatg
     5641 aaaaaattct tccgccctga attccttaac cgcttcaacg gcatcgttga attcttacat
     5701 ttagataaag atgcattgca agatatcgtc aacttattat tagacgatgt acaagttaca
     5761 ttagacaaaa aaggtattac gatggacgtt tctcaagatg cgaaagattg gttaattgaa
     5821 gaaggctatg atgaagaatt aggtgcacgt ccattaagac gtattgttga acagcaagta
     5881 cgtgacaaaa ttacagatta ctatttagat catacagacg ttaaacatgt ggatatagat
     5941 gttgaggata acgaattagt cgtaaaaggt aaataacgac actttaacat atcgcgcatc
     6001 aaaaatgagc atcaggtcgc ccttgcctgt gctcattttt ttaattattt ccctggaaaa
     6061 tgattcgctg tgtgctgttc tgttccacaa caatcacgat taatgtcaca tgtaccacat
     6121 tttccttgtt ttgaacgctt gaaaaatttt actagtgtat ataaggcata tccgaaaatt
     6181 gctaaaaaaa ttaaaatgtt aataatgact gacactttaa ccactcctta aacaaataaa
     6241 tgtccgactt gataaaaaat gaatgttaag acatatgcag tgactagagg ataggcaact
     6301 gcaagtgccg tccatttcca tgaataagtc tctttacgga ttgctgctac tgtagaaaca
     6361 caaggaatat acaatagtat aaatatcata aatgcatacg cagatagcgg tgtgaattga
     6421 ttttgaatca cattaacaag gcctgcatca cctgatgaat agataatcgc catcgaactt
     6481 acgataactt cttttgctaa aaatcctggc actaacgtag cacctgcttg ccatgttcca
     6541 aatccgagcg gttgcactaa cataccaaag aaactaccaa ccatatgtaa aaaactttga
     6601 ttgatattca cattgatacc atgtggtcct acataactta atagccaaat gactactgag
     6661 ccgccaaaaa taaatgtacc tgctttacga acaaagccct tagccttttc ccaagtacta
     6721 cgccacaacg ttttaatgga aggcacacgg tatgttggca attccacaat aaagattgca
     6781 ttatcatttt ttaaaatcgt cttagtaagt actgtactga ctaaaaatgc cataataata
     6841 cctaaaacat acaggcttaa tactactaaa gattgattct ctttgaaaaa gatacctacg
     6901 aacaacgcat acactggcag tctagcagag catgacatga atggtgcaat taatatcgtt
     6961 gttaaacgct ctttttcatt ttcaatactg cgcgcagcca taatactcgg tacattacaa
     7021 ccaaatccga taatcattgg tataaaagac ttcccgctta aaccgaacga ttccataata
     7081 cgatccatta aaaccgcaat acgtgccata taacccgaat cttctaataa agatataaag
     7141 aaaaagagca caacaatttg tggtacaaag actaatactg atcctacacc agcaataatg
     7201 ccatctgtaa ttaaatcttg taaaaatggt ataacaccaa gataattcat aatcgtcttc
     7261 acactatctg taaatgtacc acctataaat gcatcgagtt gatccgacaa aggtgtgcca
     7321 atccatgtaa atgtagtttg aaagatcaac cacataattg ctagaaagat aggcatccct
     7381 atatatttat gtgttaatat cttgtctatt ctagagctga aatattgctt atcttcatct
     7441 ggatacgtta ccacgtcttg caataacgtc tcaatataat gattgcgtat acgctccatc
     7501 tctcgacgaa cagatacagc ccctacttgt tcagcaactt gatcacgtaa actcgacaat
     7561 ttatttacaa cctctgaatt aagttcgttt gcaatttgca tgttatttaa taagaattga
     7621 atcgcaataa acctagcttg atacttatca tgagatgtct ctgtcattat tatttgacac
     7681 atatttttaa ttgtctcttc aatcttctca ccataattga ttttaaaatg cggttgatac
     7741 ccttccccta gatgctttat ttcgccaagt aaatattttg ttcctttgcc tgtacgtgcc
     7801 acaactggaa aaataggtgt ttttaacttt ttcatcaatt tatgataatc gatttttatc
     7861 ccgcgctttg tagctacatc aatcatattt aatccgatgt atattggttg attaagttct
     7921 aacaattgta ctgttaattg catatttctt tttagttgac tcgcatcaac aatgttaatg
     7981 attcctgaaa atgaatcgtt taataaatag tctgtcacta cagtttcatc tttagaaatc
     8041 ggcgataaat catatgtacc tggtaaatca attaattgtc ctacattttc tttaagtttc
     8101 cctacttttt tctctaccgt tacgccactc cagttgccta tatattcata cgaaccagtt
     8161 aaagcgttaa acaaagatgt tttaccaaca ttaggatttc ctaaaataca ataattttcc
     8221 attcgtccgg ctcctattct tctaatgcaa tagaacaagc atcgcaatgt ctaatactta
     8281 actgttgtcc gtttacttca ataatacatg gccctttaaa taaacatttt tgtttaatcg
     8341 ttatgatagc gtcatctgtt aacccaaagg cacttagacg atacaacata ttctcattag
     8401 caatatccat tcgctttatt ttataagcct tattcatttc accattttta atgtttaaca
     8461 tactatttcg ctctcctatt agaaataata atcattatca cttaaaaatc ataaccctta
     8521 aaattgtagc tcgcaatact ttatttaaat aattttcatt tttcatgtaa aatttgtgac
     8581 attgcaaaaa tgttttgtat aatcacttcc atgcaaaaaa cctccgcttc aaatgtcatt
     8641 gatagacatt taaagcggag gcatgtacat gttattttat gatttatttc gtagattttt
     8701 ttctaacaat tgattgagct tttcaatatt tttattcaac tgttctatat tcgttgcttg
     8761 tttttttgaa tttagttgat gcttgttttg ataatctatt aaagcatcat ataagaacaa
     8821 tgaacttgct gattgctgtt tcacttcttg gtacaatgcc atcgttttat catctagtac
     8881 tattgaatgt gcatcatctt gcccatgtgc atcttgatgt ttcgatacat ttgttgcacc
     8941 ttgtggatat actttaaagg ttgtgtcgta tgtttcctta ctatctttag cttcagattc
     9001 ctgtgattca accgttttat atttttcaag tgcatgtcct tcaatatcaa ctcgtggaat
     9061 aatgcgattc aaccatgctg gtaaatacca cgaacctttt ccaaacaatt tcgttaatgc
     9121 aggaattaac atcattctga ctacgaaggc atcaaagagt acaccaaacg ctaatgccat
     9181 acccattgat ttaatcatga catcttcttg gaatacaaac gcaaagaaga cactaaacat
     9241 aattaatgca gctgctacaa taacaggacc gctttctttc aatcctactt tgatagaata
     9301 atcattatcc cctgttttac tatattcttc atgaattcgc gacataagga agacttcata
     9361 atccatcgct aatccaaata agatacctat agtaataacc ggtaaaaatg ctagcattgg
     9421 tcctgtcgtt tcaataccaa acagaccttt cataaaacca tcttgcatta ctaatgttgt
     9481 aaatcctaat gttgccatta atgacaagac gaatcctaaa actgctttta atggtattag
     9541 aattgaacgg aagacaatca ttaataagaa aaatgctaat acaacaatga ctgaggcaaa
     9601 taaaggtatc gcctcattta actttttaga catatcaata ttaatgacac tttgtcccga
     9661 aatctccgtt ttgaacccat atttatcttg tgcatcttta tgataatctc gtaaatcatg
     9721 cactaaatca tttgtactct ctgcattagg cccttgctta ggtatcacga ccatcaaagc
     9781 gtaatcatta tctttactca tttgtggtgg cgtaacgata tctacatttt tcttatcttt
     9841 aatatcttta tatacagact gtaaatcttg ttgtaatcct tgtggatcat cctttttatc
     9901 tttcacattt atcaacatcg gtatttggcc attaaatcct tcaccaaatt tatccgagat
     9961 aatatcgtaa gcttttttct gtgtagaatc tgctggttta acaccgtcat ctggaatacc
    10021 aagtcgcata tgactaactg gtattgcagc tgctactaat atgattaaac ctagtaatac
    10081 tgccgcaagt gcatttcctg taataaattt agaccatggc gtatcaatat cttttttgaa
    10141 tttagactgt aatttattca ctttaatgcg tttatggaaa atgcttatta atgcaggtaa
    10201 taaagttaaa gcgctaagta ctgcaaaaac aacactaatt gccgaagcaa atcccattac
    10261 cgctaagaag tcaatgccta ctaatgataa accacatact gcaattacaa ctgttacacc
    10321 agcaaaaaca actgcactac ctgctgttcc tattgcaaga ccaatgcctt taatgtaatc
    10381 tgtttcagtt ttcataactt gtcgatatct gaataaaata aataatgcat aatcgatacc
    10441 aactgctagt ccaatcatta cggctaatgt cagtgtgaca tttggtatat cgaatgcata
    10501 agttaacaaa ctgataatac ctacaccaga ggctagacca atcaatgcac ttataattgg
    10561 taatcctgca gcaatgactg aaccgaatgt gattaacagt acaacaaatg caacaataat
    10621 accaactagt tcagaattac cgcctacttc tgtagatgtc attcctgtac cagttaactc
    10681 tgtttgtaca ttatgattgt cacgtaagtc tttcaaatga cttttaacat tatctctaga
    10741 gccatctttt aaagatgttt gactaacgtc atatgtgata tctgcaaatg cagttgtttt
    10801 atctttacta atttgcttat tttcataagg atctgatatt ttatcaatgt gcttgtcatc
    10861 ttttttaata tcatctaacg ttttcttaat atctttagta atgttcggtt gcacaatacc
    10921 atcatcttta gtcgtcttaa agacaacacg tatttgtgcc ttttcactat cttgattaaa
    10981 atgtttttca atctttttat tcgtatctaa cgactctaat cctgtcattt taatatcatt
    11041 gtcaaatttc ggtgcatttg tagcaagtgg tatcaatatt gcagctacaa tcactatcca
    11101 tgcaatgacc gcggaccatt tatgttttgc gatgaatgtc cccatcttat ataaaaattt
    11161 tgccaaagta tattgcctcc ttttaaaatc aacgttatag tttaaatata cagtgtagat
    11221 tattgttcga ttatagtatc tatccccgac ctcttaaaga atcaattgga aaattttgta
    11281 tattaaacta cacacaaagg agaaatgtag atgaaagaga ctgatttacg agttataaag
    11341 acaaaaaaag cattgtcgag tagcttgcta caattgttag aacagcaatt attccaaacg
    11401 attactgtca atcaaatttg cgacaacgca ctcgtacacc gtacaacatt ttataaacat
    11461 ttttatgata aatatgatct tctagagtac ttgttcaatc aattgactaa agactacttt
    11521 gctagagata tcagtgaccg tcttaatcat ccattccaaa cgatgagtga tacgattaat
    11581 aataaagagg atttgagaga aatcgcagaa ttccaagaag aagacgctga atttaataaa
    11641 gtattaaaaa atgtctgcat taaaattatg cataacgata tcaaaaataa tagagaccgt
    11701 atcgatattg acagcgacat cccagataat ctcatatttt atatttatga ctcgttgatt
    11761 gaaggtttta tacattggat aaaagatgaa aaaattgatt ggcctggcga agatattgat
    11821 aacattttcc atagattaat caatattaag attaaatagt agatgagaaa ctcatgagcg
    11881 ttaccaacat tcataataaa aacgatagtg tacacgttaa tgaattcgtg tactactatc
    11941 gttttttatt tttatcgtgc ttatcgctat taaaacaact gatacacaac acataaacta
    12001 tgaagaaaaa aataaatccg ctatctaaat gactttgact cagttgttta aatgaccaaa
    12061 ttgctaatac aattcccatt attattgaaa taacgtatct cacattctta tacctataat
    12121 ccttttctaa aaatatggtt gctattactt aatttttaaa gttataaata aaaagagcca
    12181 accgcaatgg atggcccttg ttcattatga agcattagaa catttctgaa acaacctttt
    12241 gttctaagaa gtgtaataag tagtctggac tacctgtttt agcgtccgta cctgacattt
    12301 tgaaaccacc aaatggatgg tatccaacaa ctgctgaagt acagcctctg ttaaggtata
    12361 aattgcctac atcaaattcg tttaccgctt taatccaatg ctcgcgatta tttgtaatca
    12421 ctgcaccagt taaaccgtaa tctgtatcat ttgcaacctc aattgcttca tcaaaatcgt
    12481 taactttcac aaagccaaca actggaccaa aaatttcttc ttgcatgatt ctatctttag
    12541 atttaagtcc tgaaatgatt gttggttcta caaagtaacc ttttgaatca tcagtgccgc
    12601 caccttgttc taatttacct tcttctttac caatctcaat ataattttta atcttatcaa
    12661 attgtttttt attaataact gggcccatat acgtattgtc tacagtattg cccaacgtta
    12721 attcttttgt taatttgatt gatttctcta atacttcgtc ataaacgtct ttatgcacaa
    12781 ttgcacgtga acatgctgaa catttttgac cagaaaaacc aaatgctgac gttacaatag
    12841 cttctgctgc catatctgta tcaatatttt catcaactac aatggcatct ttaccaccca
    12901 tttcagcgat aacacgtttc aagaagtttt gaccttcttg aacaacggca ctacgttcat
    12961 aaattctagt acctgtcgca cgtgatcctg taaatgtaac gaaatgcgta tctttatgat
    13021 caactaagta atcaccaatt tctttcggat caccaggaac aaagttaact acgccttttg
    13081 gtaatcctgc ttcttctaaa atttccatta atttataagc gatataaggt gtatcctcag
    13141 caggtttcaa taacactgta ttacctgcca caactggtgc taaagttgta ccagccataa
    13201 tcgcaaacgg gaagttccac ggcggaattg taacacctgt accaattgat ttatagaaat
    13261 atttattgtg ttcaccttca cgatcaagta ctggcttacc ttgagccaag tccatcattg
    13321 aacgtgcata gtattcaata aaatcaatac cttcagctgc atcaccaact gcttcatccc
    13381 atggcttacc tgcttcataa accataattg ctgcaatttc cgcttttcga cgacgaataa
    13441 ttgccgaaac acgtaacata agctctgcac gatcatttgc tgaccatgtt ttccaagatt
    13501 tataagcttc gtttgctgct ttaaacgcat cttcaacatc ttgttttgtt gcctttgatg
    13561 catttgcaat cacttgtgat gtgtctgcag gattgattga tttaattttg tcatctttga
    13621 aaatcttctc tccattaatc actaatggta tgtcttgacc taattctttt tccacgtctt
    13681 tcaatgcttt cttaaacata tccacatttt cttggactga aaaatcgtaa ccaggttcat
    13741 ttttaaattc tactaccatg tacacttacc ccctataaat tttgaaagtg gtttaaccct
    13801 ttgatttaat gatataacat catttaaact cattttacta tgattaaggt tagttttgca
    13861 atcgctttca tttttatgtt ttatcactta ttctcaagta ttttgaaatt gattggttac
    13921 tttttaaaat ttatatgggt cgcaactgct actttatcgt ttcgtcattt aatgtttcgg
    13981 atggtaggtc attatcaatt ttacgaacga ctttacaagg gtttccaacc gctaagctgt
    14041 gtggcggaat atctttagtg acaacactac cagcaccaat cacactgcct tctccaatcg
    14101 tcacccctgg taacacggct acatgaccgc caaaccaagt attactgcca atatgaatgg
    14161 gtccggcttt ttcaaaacct tcatttctat gatggaaatt aagtggatgt gtcgctgtgt
    14221 agaatccaca attaggtcct ataaaaacat tatcgccaat tgtaatctgt ccaccatcca
    14281 taaagtaaca attggtgttt acatagacat tttttcctag tttaacgttc caaccataat
    14341 ctgtatcaaa aggaatcgaa atacttacat tgtctgttgt tgtttgaaat aattgatcaa
    14401 ttaattcctt tcttttattt gtagcactcg gtcttgtatg atttaattca aagcaaatat
    14461 ctttcgctcg tgcacgttca ttgattaagt attgatcaaa gtttgcatcg taccattttt
    14521 ctgctaacat tttttctttt tcagtcatta cacctttcaa ctcctaataa cttatttact
    14581 tgtttaaaag ttaatcaaat aaaccttcgc ctatgcaact aatacgctat aacattatga
    14641 aatcatgacc ttatcaccct tatctataca attctcgcat caaatactgc taaagtagta
    14701 gataaattca atactacaga cgcattcatt ttttaatcta ttaacgtaca atgtgagtaa
    14761 gagaaatata aaggagtatg atagcgatga gaatattaat tacaggcaca gttgctatct
    14821 taatcattct aggtttggtc aaaacgatac aagattacga aatgacaaac gacacgagtc
    14881 gtcagttgtc agacaacaaa gatgatgata aagtcatcca tcttaataat tttaaaaatt
    14941 tacatgcgaa agaatttaac ccatctgatt tcttttaagt cacctaagaa ttgcaaatcc
    15001 agaagtcatt taagttttac ctttcattca tacatccttt aatattaatt acgacttctt
    15061 ttatatagat gctaagtaga gagattgttg tgcaatgttt gcacggcaat ctctcttttt
    15121 ctttttaaaa ttggtaaaag taaaacgcaa cgattgactt atatacctat agggggtaca
    15181 ttagacgtgt aacaatgaat cacagggagg caataatgtg gctaatacga aaaaaacaac
    15241 attagatatc actggtatga cttgtgccgc atgttcaaat cgtatcgaaa agaaactgaa
    15301 taaacttgat gacgttaatg cccaagtgaa tttaactaca gagaaagcaa ctgttgagta
    15361 taaccctgat caacatgatg tccaagaatt tattaatacg attcaacatt taggttacgg
    15421 tgtcgctgta gaaactgtcg aattagacat tacaggtatg acttgtgctg catgctcaag
    15481 ccgtattgaa aaagtgttaa ataaaatgga cggcgttcaa aatgcaacgg tcaatttaac
    15541 aacagagcaa gctaaagttg actattatcc tgaagaaaca gatgctgata aacttgtcac
    15601 tcgcattcaa aaattaggtt atgacgcgtc tattaaagat aacaataaag atcaaacgtc
    15661 acgcaaagct gaagcgctac aacataaatt gattaagctt atcatatcag cagtattatc
    15721 tttaccacta ttaatgttaa tgtttgtaca tcttttcaat atgcatatac cagcactatt
    15781 tacgaatcca tggttccaat ttattttagc tacacctgta caatttatta ttggatggca
    15841 attttatgta ggtgcttata aaaacttaag aaatggtggc gccaatatgg atgtacttgt
    15901 tgctgttggt acaagtgcag catattttta cagtatttat gaaatggttc gttggctaaa
    15961 tggctcaaca acgcaaccgc atttatactt tgaaacaagc gccgtactaa ttaccttaat
    16021 cttattcggt aagtatttag aagctagagc gaagtctcaa acaaccaatg cgcttggcga
    16081 attattaagt ttacaagcta aagaagcacg cattttaaaa gatggtaatg aagtgatgat
    16141 tcctctaaat gaagtacatg ttggagatac acttatcgtt aaaccaggtg aaaagatacc
    16201 tgttgatggc aaaattatta aaggtatgac tgccatcgac gaatctatgt taacaggtga
    16261 atctatccct gttgagaaga atgttgatga tactgtaatt ggttcaacga tgaacaaaaa
    16321 cggtactatt actatgacag caacaaaagt tggcggggac actgcgttgg caaatattat
    16381 taaagttgtc gaagaagctc aaagttctaa agcgccgatt caacgattgg cagatattat
    16441 ttctggttat ttcgttccta tcgttgttgg tatcgcacta ttaacattta tcgtgtggat
    16501 tactttagtt acaccaggta catttgaacc tgcacttgtt gcgagtattt ccgttctcgt
    16561 cattgcttgt ccatgcgcat tgggacttgc tacaccaact tctattatgg taggtactgg
    16621 tcgcgctgct gaaaatggta ttttatttaa aggtggcgag tttgttgaac gcacacatca
    16681 aattgatacc atcgttttag ataagacggg taccattaca aatggtcgtc cagtcgtgac
    16741 agattatcat ggtgacaatc aaacgctaca actacttgct actgctgaaa aagattctga
    16801 acacccattg gcagaagcca ttgtcaatta tgcaaaagaa aagcaattaa tattaactga
    16861 gacaacaaca tttaaagcag tacctggcca tggtattgaa gcaacgattg atcatcacta
    16921 tatattggtt ggtaaccgta aattaatggc tgacaatgat attagcttgc ctaagcatat
    16981 ttctgatgat ttaacacatt atgaacgaga tggtaaaact gctatgctca ttgctgttaa
    17041 ttattcatta actggtatca tcgcagtggc agatactgtc aaagatcatg ccaaagatgc
    17101 tataaaacaa ttgcatgata tgggcattga agttgccatg ttaactggcg ataataaaaa
    17161 cactgctcaa gccattgcaa aacaagtagg catagatact gttattgcag atattttacc
    17221 agaagaaaaa gctgcacaaa ttgcgaaact acagcaacaa ggtaagaagg ttgcgatggt
    17281 tggtgacggt gtaaatgatg cacctgcatt agttaaagct gatatcggta tcgccattgg
    17341 tacaggtaca gaagttgcca ttgaagcagc tgatattact attcttggtg gcgacttgat
    17401 gcttattcct aaagccattt atgcaagtaa agcaaccatt cgtaatattc gtcaaaatct
    17461 attttgggca ttcggctata atattgccgg tatccctata gctgcattgg gcttacttgc
    17521 gccatgggtt gctggtgctg caatggcact aagttcagta agtgttgtca caaacgcact
    17581 tagattgaaa aagatgcgat tagaaccacg ccgtaaagat gcctagattc cttaataatg
    17641 aaggattcgt tggtgattct gagataggct agtgattggc tctataatgt c
//
LOCUS       DAKTIO010000043        17511 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.043, whole genome shotgun sequence.
ACCESSION   DAKTIO010000043 DAKTIO010000000
VERSION     DAKTIO010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17511
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.043"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            32..739
                     /locus_tag="O0293_001071"
     CDS             32..739
                     /locus_tag="O0293_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCY0279963.1"
                     /translation="MVVLLSYSCSCIFSFLYQFISIEETSFDYLHRRSKCDYCNSSLK
                     WYELMPIISFLLLKGRCRNCRKRISLTHFLGETFALIPIVFIKYDFTYVNATLFITTY
                     VFLLIFTMTDITSLMLDCRLIIIYCIVSLSLSMIYPVAFIIISMTTHIFYFLFRAYIG
                     YGDVLLISALSLFFPLQFTIYVILFTFVIAGLVALITMIFKPIKLLPLVPFIFISFFI
                     NSLFYNDIHQFLGGVYF"
     gene            736..1422
                     /gene="radC"
                     /locus_tag="O0293_001072"
     CDS             736..1422
                     /gene="radC"
                     /locus_tag="O0293_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCY0279964.1"
                     /translation="MKIKEMVTSEMPRERLLSHGAKSLSNTELLAILINTGRKGFSSI
                     DISNELLKSASNLNELKKSSINDLIKVKGIGLQKAITLKAAFELGERMGRRAENNRIK
                     ITQPSDVADYMIPTMKDLTQEHFVILLLNSKNVVIKETCVFKGTLNSSIVHPREIFSI
                     AVRENANAIIAVHNHPSGDVTPSQEDIITTMRLKECGLILGIDLLDHIIIGDNRFTSL
                     VEAGYFDEND"
     gene            1490..1774
                     /locus_tag="O0293_001073"
     CDS             1490..1774
                     /locus_tag="O0293_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279965.1"
                     /translation="MTLFIIIGVLVPMVYTMQLNIKNEPVTKRNLLITLALSTLGILV
                     TALAGVIVTKQAFPLLSVAIGSIFTGIVWGLLLSGSYALIRFLSNAFGRK"
     gene            complement(1891..2088)
                     /locus_tag="O0293_001074"
     CDS             complement(1891..2088)
                     /locus_tag="O0293_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279966.1"
                     /translation="MSILTIILIALLVILLFRVGLSILRFLIYVGLVLLCIYLGYQGL
                     IWLLDFFQINSGFLPHFQFNN"
     gene            2248..2721
                     /locus_tag="O0293_001075"
     CDS             2248..2721
                     /locus_tag="O0293_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4930 family protein"
                     /protein_id="HCY0279967.1"
                     /translation="MRLIFSMIKNIIAVIAIVIIVYIALKYAPFLKEQDWNPVNHDND
                     QMNQVTQPTNDAQHVYVSGEKYSLKENDLIKNVPLSQIKNVFKMIDKQEFMAVSGMNR
                     MAYNDQYIIGQRGDEFILYKFGDESMRVYNTEFEMQQDLNELGQNLQLKSENAYQ"
     gene            2795..2914
                     /locus_tag="O0293_001076"
     CDS             2795..2914
                     /locus_tag="O0293_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279968.1"
                     /translation="MAEQSKQKQANEQQKAQNLFARWRQLQNSNSESSNDTNK"
     gene            3114..3956
                     /gene="mreC"
                     /locus_tag="O0293_001077"
     CDS             3114..3956
                     /gene="mreC"
                     /locus_tag="O0293_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCY0279969.1"
                     /translation="MLKFFKNNKLIVVLCAIIVFIALIGLSIRSQSQSPPEQYIGDSV
                     SFGQRVVSYPVNFVAGTIGDFFKKGDSKESKNKISQLESKNQQLEAENEKLKKELDLK
                     DISKFDPISTTVLARNPDQWMNTIVIDKGSKSGITSNMAVMTSQGFVGRVTKVNKFSS
                     QVDLISTNTRAGKLSVNIQHGSKNIFGLIDRYDEKNSELVISDINNRDNISKGDKVVT
                     SGLADQLPSNLYIGEVTKVQNDQYGLAKEVRVKTGADLTDLSHVYVAKRDPKTIPDDE
                     SRDK"
     gene            3956..4486
                     /gene="mreD"
                     /locus_tag="O0293_001078"
     CDS             3956..4486
                     /gene="mreD"
                     /locus_tag="O0293_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCY0279970.1"
                     /translation="MRTLYYFLIGILLFYIDTAIGLLIPMHIGKFELVFVPHLTFMYI
                     LMMVVYRGFGVSLLLSIFLGVMTDVYFGSIYGVYLFGYILFLALIDRFFKIFYKDHSM
                     LFIIILASTLLLEVYVALIYGMLGFIQFDIIHFVVFRLLPTLIMNFVLLIMLYPLIIK
                     FLKKTNNDIDMKRRQW"
     misc_feature    4513..4640
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4653..4961
                     /gene="rplU"
                     /locus_tag="O0293_001079"
     CDS             4653..4961
                     /gene="rplU"
                     /locus_tag="O0293_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCY0279971.1"
                     /translation="MFAIIETGGKQIKVEEGQEIFVEKLDVNEGDTFTFDKVLFVGGD
                     SVKVGAPTVEGATVTATVNKQGRGKKITVFTYKRRKNSKRKKGHRQPYTKLTIDKINA
                     "
     gene            4967..5287
                     /locus_tag="O0293_001080"
     CDS             4967..5287
                     /locus_tag="O0293_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HCY0279972.1"
                     /translation="MITVDITVNDEGKVTDVIMDGHADHGEYGHDIVCAGASAVLFGS
                     VNAIIGLTSERPDINYDDNGGHFHIRSVDTNNDEAQLILQTMLVSLQTIEEEYNENIR
                     LNYK"
     gene            5299..5583
                     /gene="rpmA"
                     /locus_tag="O0293_001081"
     CDS             5299..5583
                     /gene="rpmA"
                     /locus_tag="O0293_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCY0279973.1"
                     /translation="MLKLNLQFFASKKGVSSTKNGRDSESKRLGAKRADGQFVTGGSI
                     LYRQRGTKIYPGENVGRGGDDTLFAKIDGVVKFERKGRDKKQVSVYAVAE"
     gene            5938..7230
                     /gene="obgE"
                     /locus_tag="O0293_001082"
     CDS             5938..7230
                     /gene="obgE"
                     /locus_tag="O0293_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HCY0279974.1"
                     /translation="MFVDQVKISLKAGDGGNGITAYRREKYVPFGGPAGGDGGKGASV
                     VFEVDEGLRTLLDFRYQRHFKASKGENGQSSNMHGKNAEDLVLKVPPGTIIKNVETDE
                     VLADLVEDGQRAVVAKGGRGGRGNSRFATPRNPAPDFSEKGEPGEELDVSLELKLLAD
                     VGLVGFPSVGKSTLLSIVSKAKPKIGAYHFTTIKPNLGVVSTPDQRSFVMADLPGLIE
                     GASDGVGLGHQFLRHVERTKVIVHMIDMSGSEGREPIEDYKVINQELAAYEQRLEDRP
                     QIVVANKMDLPESQDNLNLFKEEIGEDVPVIPVSTITRDNIDQLLYAIADKLEEYKDV
                     DFTVEEEESVGINRVLYKHTPSQDKFTISRDDDGAYVVSGNAIERMFKMTDFNSDPAV
                     RRFARQMRSMGIDDALRERGCKNGDIVRILGGEFEFVE"
     gene            7243..7698
                     /locus_tag="O0293_001083"
     CDS             7243..7698
                     /locus_tag="O0293_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HCY0279975.1"
                     /translation="MDNKDYKKFYLIREDVLPESVVKTLKIKDALKSDPTLSIYDAVK
                     QFDLSRSAFYKYRETIFPVDDKMLDHREFTLILYVTDIVGMLARVLDVISKLELSVLT
                     IHQSIPMEEKATITLSLNAKSKETSVEDVIGALRNLDYVSKVELISMSM"
     gene            7712..8314
                     /gene="ruvA"
                     /locus_tag="O0293_001084"
     CDS             7712..8314
                     /gene="ruvA"
                     /locus_tag="O0293_001084"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCY0279976.1"
                     /translation="MYAYVKGKLTHLYPTHVVVETAGVGYEIQTPNSYRFQKHLDHEV
                     LIHTSLIVREDAQLLYGFSSEEEKDMFLSLIKVTGIGPKSALAILATSTPNEVKRAIE
                     NENDTYLTKFPGIGKKTARQIVLDLKGKVKITEEDSDSLLQVDATSTVQDQFVQEAML
                     ALEALGYSKRELAKVEKTLNKNKYDSVDEAVKAGLQLVVS"
     gene            8346..9350
                     /gene="ruvB"
                     /locus_tag="O0293_001085"
     CDS             8346..9350
                     /gene="ruvB"
                     /locus_tag="O0293_001085"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCY0279977.1"
                     /translation="MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIK
                     AAKLRHEPLDHVLLFGPPGLGKTTLSNIIANEMEVNIRTVSGPSLERPGDLAAILSGL
                     QPGDVLFIDEIHRLSSVVEEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATT
                     RAGSLTGPLRDRFGVHLRLEYYNESDLKEIIIRTAEVLGTGIDEESAIELAKRSRGTP
                     RVANRLLKRVRDFQQVNEDEQIYIETTKHALGLLQVDQHGLDYIDHKMMNCIIKQYNG
                     GPVGLDTIAVTIGEERITIEDVYEPFLIQKGFLERTPRGRKATPLAYEHFAKSNEERE
                     "
     gene            9352..10377
                     /gene="queA"
                     /locus_tag="O0293_001086"
     CDS             9352..10377
                     /gene="queA"
                     /locus_tag="O0293_001086"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCY0279978.1"
                     /translation="MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFK
                     DIIEYFRPGDTLVLNDTRVMPARLFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRI
                     KVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDD
                     PDRYQTVYAKESGSAAAPTAGLHFTDELLIEIKNKGVNIAFVTLHVGLGTFRPVSVDD
                     VNDHEMHSEYYQMTQETADLLNDTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGW
                     TNIFIYPGFDFKAIDGQITNFHLPKSTLVMLVSAFSSRENVLNAYKTAVNLEYRFFSF
                     GDAMLII"
     gene            10400..11539
                     /gene="tgt"
                     /locus_tag="O0293_001087"
     CDS             10400..11539
                     /gene="tgt"
                     /locus_tag="O0293_001087"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016425271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCY0279979.1"
                     /translation="MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVK
                     TMSPEELRQIEAKIILGNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFS
                     LSNLRKITEEGVEFRHHTNGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYV
                     KKSIERTTRWAKRCLDAHQRPEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGG
                     LSVGEPKPVMYKMVEHTEQFMPKDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIA
                     RNGTCMTSQGRLVIKNAKFADDLRPLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLT
                     TIHNLHFLLKLMEDIRQAIREDRLLDFKEEFFEQYGLNVENPKNF"
     gene            11558..11818
                     /gene="yajC"
                     /locus_tag="O0293_001088"
     CDS             11558..11818
                     /gene="yajC"
                     /locus_tag="O0293_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCY0279980.1"
                     /translation="MQFSLLIYIVVIFAVMYFLMIRPQQKRAKQHRELINNIQSGQRI
                     TTIGGIKGTVKAVDETTVVITVNGHGTELTFEKPAIKQVDPS"
     gene            12093..14372
                     /gene="secDF"
                     /locus_tag="O0293_001089"
     CDS             12093..14372
                     /gene="secDF"
                     /locus_tag="O0293_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HCY0279981.1"
                     /translation="MKKSSRIIAFLLLVVLLFAGMAATYKSVIKNVNLGLDLQGGFEV
                     LYQVDPLNKGDKIDKKALQSTAQTLENRVNVLGVSEPKIQVEEPNRIRVQLAGVTDQN
                     EARKILSSQANLTIRDAEDKVKLSGSDIKQGSAKQEFKQETNQPTVTFKVKDKNKFKK
                     VTEEISKKRDNVMVVWLDFKKGDSYKKEAQKKNPKFISAASVDQPINSDSVEISGGFK
                     GQEGVKKAKQIAELLNAGSLPVDLKEIYSNSVGAQFGQDALDKTVFASFIGVALIYLF
                     MLGFYRLPGLVAIIALTTYIYLTLVAFNFISGVLTLPGLAALVLGVGMAVDANIIMYE
                     RIKDELRIGRTIKQAFSKANKSSFLTIFDSNLTTVIAAAVLFFFGESSVKGFATMLLL
                     GILMIFVTAVFLSRFLLSLLVSSNIFKNQFWLFGVKKNKRHDINEGVDVHDLKTSFEK
                     WNFVKLAKPLIGVSILIVVVGLVILYIFKLNLGIDFSSGTRVDFQSKQAITQQKVEQV
                     VKDSGLKADQIQINGKDNKVATVQFKDDLTRAQDNKLSDNIKSKFGDTPQINTVSPII
                     GQELAKNAMLALIYASIGIIIYVSLRFEWRMGLSSVLALLHDVFIIVAIFSLFRIEVD
                     LTFIAAVLTIVGYSINDTIVTFDRVRENLQKVKVITTTEQIDDIVNRSIRQTMTRSIN
                     TVLTVIVVVVAILFFGAPTIFNFTLALFIGLISGVFSSIFIAVPLWGIMKKRQLKKSP
                     KHKLVVYKEKKSNDEKILV"
     gene            14575..16848
                     /gene="recJ"
                     /locus_tag="O0293_001090"
     CDS             14575..16848
                     /gene="recJ"
                     /locus_tag="O0293_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCY0279982.1"
                     /translation="MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQ
                     AIESIISDTDINHDALQLSDMTKTIERIKRAIANDEKILVYGDYDADGVTATTILVIT
                     LQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLG
                     VDVIVTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFK
                     ALVAIGTIADLVSLTDENRSLVKQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFI
                     IGPRLNAVGRLDDASLACELLMTDVEEEAAFLAEQVEHFNRERKDIVATITEEAMAMA
                     ETKVKKGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSID
                     QVSMFEILSAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVK
                     QVDVLLTENDITIKNIRDMNRLRPFGTDFSRPIFEMDDLSVSSVKAIGQQKNHLKLTL
                     GESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQIIIQDIAMNEQQILD
                     YRSKRKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPTSMEDL
                     SNSLQQLQFSQLYIVLQHNHSIYFDGIPNMDIFKKCYKALITKQETNIQKEGMLLCQH
                     LSVKPDTLKFMLKVFLDLKFVTQEDGLIRINQQPDKRSIDSSKVYQLRQQRMDVEKQL
                     LYQDFSEIKNWIKSQLS"
     gene            16870..17388
                     /locus_tag="O0293_001091"
     CDS             16870..17388
                     /locus_tag="O0293_001091"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009559512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCY0279983.1"
                     /translation="MDLKQYVSEVQDWPKPGVSFKDITTIMDNGEAYGYATDKIVEYA
                     KDRDVDIVVGPEARGFIIGCPVAYSMGIGFAPVRKEGKLPREVIRYEYDLEYGTNVLT
                     MHKDAIKPGQRVLITDDLLATGGTIEAAIKLVEKLGGIVVGIAFIIELKYLNGIEKIK
                     DYDVMSLISYDE"
BASE COUNT         6241 a         2386 c         3307 g         5577 t
ORIGIN      
        1 ctaactataa tttacataag gaggttgtca tttggtagta ttgttatctt atagttgcag
       61 ttgtattttt agttttctat atcaatttat atctatagaa gaaacgtcat ttgattattt
      121 acatagaaga tcgaaatgtg attattgtaa ttcgtcactc aaatggtatg aattaatgcc
      181 gattattagt tttttattat taaaagggcg atgtcgaaac tgtcgaaagc gtatttccct
      241 aacacatttc ttaggggaaa cctttgcttt aatacctatc gtctttatta agtatgattt
      301 cacatacgta aatgctacgc tatttataac tacatatgtt tttctgctta tttttactat
      361 gaccgatatc acttctttaa tgttagattg tcgcttaatt ataatttatt gtatcgtttc
      421 tctctcgtta agtatgattt atccagtagc ttttatcatt attagtatga ccacgcatat
      481 attctacttt ttatttcggg catatattgg ttatggtgac gttttactaa tatctgcact
      541 ttctttgttt ttccctctcc aattcactat ttatgtcatt ttatttacat ttgtcattgc
      601 tggtttagtt gctttaatta ccatgatatt taagccgatt aaactattac cccttgttcc
      661 atttatattt atttcctttt ttatcaattc acttttttat aatgatatcc atcaattttt
      721 aggaggcgta tatttttgaa aattaaagaa atggtaactt cagaaatgcc aagagaacgt
      781 ttgttaagcc atggtgcaaa aagtctttcg aatacagaat tattagctat attaattaac
      841 accggaagaa aaggattctc gagcatagac attagtaatg aactgcttaa atctgcttcg
      901 aatctgaatg aattgaaaaa atcttcaatt aacgatttga taaaagttaa agggattgga
      961 ttacaaaaag caattacttt aaaagcagca tttgagttag gggaaaggat ggggagaaga
     1021 gctgaaaata atcgtataaa aataacgcaa ccaagtgatg ttgctgatta tatgattcca
     1081 acaatgaaag atttaacaca agaacatttt gtcattttat tgttaaattc aaaaaatgta
     1141 gtgattaaag aaacctgtgt ttttaaaggt acattaaata gttcgattgt acatccacgt
     1201 gaaattttta gtattgcggt gagagaaaat gccaatgcaa tcatcgcagt tcataatcat
     1261 ccatccggtg atgtaacgcc ctcacaagaa gatatcataa caacaatgag gttgaaggag
     1321 tgtggtttga ttttagggat agatttattg gatcatatta taatcggtga taatagattt
     1381 accagtcttg tagaagcggg ttactttgat gaaaatgatt gaagttgata gaattaatga
     1441 cgtatcttgt gtataatacc tacgaagtac tttcattgga ggaaaaatag tgactctatt
     1501 tattattatc ggggttctcg tgccaatggt ttataccatg cagttaaata ttaaaaatga
     1561 acctgtaaca aagcgcaatc ttttaataac attagcttta tctacgttag gtattttagt
     1621 aaccgcgtta gcaggtgtaa tcgttacgaa acaagctttt cctttattaa gtgtagcaat
     1681 tggctcaatt tttactggaa tcgtttgggg ccttttacta agtggtagct acgcgctgat
     1741 acgattttta tctaacgcat ttgggcgtaa gtaatagtaa tctgttcatc aagtagtatc
     1801 cgtgcttgaa aacaaactaa aactcctaat gtggaactag ttaaaaaatt ctaaactagt
     1861 acattaggag ttttgttatg cagaataaat ttaattgtta aattgaaagt gcggtaaaaa
     1921 tccactattt atttgaaaaa aatcgagcaa ccaaattaaa ccttgatatc ctaagtaaat
     1981 acataataaa acaagtccga cataaattaa aaatcgcaaa atagaaagtc caactctaaa
     2041 aaggaggatg actaatagtg ctattaaaat aattgttagt atactcaatg ctacaaacct
     2101 cctaatacgc ttttaaatca taacaaaatt ataagctatt ttaaatgaac gtgcatcagt
     2161 cctaagatat atcatgatta aggctgaaga ctgaggaaaa tatacataat aaaatatata
     2221 attagatgaa gacattggag gcgaactatg agattgatat ttagtatgat taaaaacatt
     2281 atagcagtaa tagcaattgt gataattgtt tatatcgcct tgaaatacgc acctttttta
     2341 aaagaacaag attggaatcc tgtaaatcac gataatgatc aaatgaatca agttacgcaa
     2401 cccactaatg atgcccaaca tgtttatgta agtggtgaga aatattcatt aaaagaaaat
     2461 gatttaataa agaatgtacc attaagccaa attaaaaatg tatttaaaat gatagataaa
     2521 caagagttta tggctgtgtc tggaatgaat cgcatggctt ataatgatca atatattata
     2581 ggtcaaagag gagacgaatt tattctttat aaatttggag atgagtcaat gcgtgtttac
     2641 aatactgaat ttgaaatgca acaagactta aatgaattag ggcaaaattt acaactaaaa
     2701 tctgaaaatg cttatcaata ggaaattatt gcataagaaa tatattttag ttatcctgtc
     2761 actagaataa atttaaaagg ttggtgtagt tcaaatggca gaacaatcaa aacagaaaca
     2821 agctaatgaa caacaaaagg cccagaactt attcgcacgt tggagacaac ttcagaattc
     2881 gaacagtgaa tcctcgaacg atacaaacaa ataattttaa aatttaaatc gattatttaa
     2941 actactagtg actggaatgt ttgctagtag tttttttgtt tttaaaatgc cacaaatttt
     3001 ttacggagtt gttcattttc aaagtaattc gtagttagtg ctttatttca taatgggagt
     3061 aatataaaat agaaaaggat atacaatatt ttgacataat agaggtgttc tgggtgctta
     3121 agttttttaa aaataacaaa ttaattgttg ttttatgtgc aattatcgtt tttattgcat
     3181 taattgggct gtccatacgt tcacaatctc aatcacctcc tgaacaatat ataggtgatt
     3241 ctgtgtcttt tggacaacga gttgtgagtt atccagttaa ttttgttgct ggtacgattg
     3301 gggacttttt taaaaaagga gattctaaag aatctaaaaa taagattagc cagttagaat
     3361 ctaagaacca acaattagaa gcggaaaatg aaaaattaaa aaaagagctt gatttaaaag
     3421 atatttcaaa atttgatcct atttctacta cggttttggc aagaaatccg gatcagtgga
     3481 tgaatacaat tgtaattgat aagggatcta aatcaggtat aacttcaaat atggctgtga
     3541 tgacatcaca aggttttgtt ggaagagtta ctaaagttaa taaattttct tcacaagttg
     3601 atttaatctc aactaataca cgtgcgggta aattatctgt aaatatacaa cacggttcta
     3661 aaaatatatt tggtttaatt gatcgttatg atgaaaagaa ctcagaactt gtaattagtg
     3721 acattaataa tagagataat atctcaaaag gtgataaagt cgttacaagt ggattagctg
     3781 atcaactacc aagtaattta tatataggag aagtgactaa ggttcaaaat gatcaatacg
     3841 gcttagctaa agaagttagg gttaagactg gtgcggactt aacagatttg agtcatgttt
     3901 atgttgcaaa aagagatcct aaaacaattc ctgatgatga aagcagggat aaataatgcg
     3961 tacactgtat tattttttga taggtatttt actattttat atagatactg caattgggct
     4021 tcttattcca atgcacatag gtaagtttga acttgtattt gtaccacacc ttacatttat
     4081 gtatatttta atgatggtag tgtatcgtgg ctttggcgta tcattattgc tcagcatatt
     4141 tttaggcgta atgactgatg tatactttgg tagtatttat ggagtgtact tatttggcta
     4201 tatattgttt ttagcactta tagatcgatt ctttaaaatt ttttacaaag atcattcgat
     4261 gttattcatt attatattag ccagcacctt attattagaa gtctatgtgg cattaatata
     4321 cggtatgtta ggattcattc aatttgatat tattcatttt gtagtcttta gattattgcc
     4381 aacattaatt atgaattttg tactgttgat tatgctttat ccgttgatta taaagttcct
     4441 taaaaaaaca aacaatgaca ttgacatgaa acgtcgtcaa tggtaagatg caatagttga
     4501 gtagttataa gtagctacat acaaccgctc aaatataggt tcaagaacat ttttaatgaa
     4561 gaaagcgatt ttcccacaga gtgcaaacgc ttgactcaat aacgaaatgt cacctatgta
     4621 tggcgtgact tattatacag gaggtgcaaa gtatgtttgc tattattgaa acaggtggaa
     4681 aacaaatcaa agtagaagaa ggtcaagaaa tcttcgttga aaaattagac gtaaacgaag
     4741 gagatacttt tacatttgat aaagtattat ttgtaggtgg agattcagtt aaagttggag
     4801 cgccaacagt tgaaggtgca acagttactg ctactgttaa taaacaaggt cgcggtaaaa
     4861 aaatcactgt attcacatac aaacgtcgta aaaattcaaa acgtaaaaaa ggccatcgtc
     4921 aaccatacac taaattaaca atcgataaaa tcaacgcgta attattatga ttactgttga
     4981 tattacagtt aatgatgaag gcaaagtaac agacgttatt atggatggcc atgctgacca
     5041 tggtgaatat ggtcatgata tcgtttgtgc tggagcttca gctgtattgt ttggtagtgt
     5101 taatgcgatt ataggattga catctgagag accagatatc aattatgacg acaatggtgg
     5161 tcattttcat ataagaagcg ttgatacaaa caacgatgaa gcgcaactaa ttcttcaaac
     5221 aatgcttgtg tctttacaaa ctattgaaga agaatataat gagaatatta gattaaatta
     5281 taagtgaggt gcattccgat gttaaaatta aacttacaat tcttcgcatc taaaaaaggg
     5341 gtaagttcta caaaaaacgg acgtgactct gaatcaaaac gcttaggtgc taaacgtgct
     5401 gacggtcaat tcgtaacagg tggttcaatt ttatatcgcc aacgtggtac taaaatttac
     5461 cctggtgaaa atgtaggtcg tggtggcgat gatacattat tcgctaaaat cgacggcgtt
     5521 gttaaattcg aacgtaaagg tcgcgacaaa aaacaagttt ctgtatatgc agtagctgaa
     5581 taattttgtc tagttaacac cagaagtgaa tcttctggtg ttttttactt tttataaaat
     5641 gtttttcatt attattttca ttatgttatt aaaaaatggg aattttagac gttatactaa
     5701 atgtgcaact gtataagagc ccctaatcac taaactataa gggggacaaa ggaatacagt
     5761 tgcagcgttt aaagaataaa ctgtaccaca attggtgctg agaaatataa gtattttaaa
     5821 gcaaagattt ataaaagtaa ctgcataaga gcccctaatt atttacaata tataaggggc
     5881 tctaatatgc tataattatt gggaaaatga aaattatatg taaaagaggt gagatatatg
     5941 tttgtcgatc aagtcaaaat atctcttaaa gccggtgatg gtggtaatgg tattaccgca
     6001 tacagaagag aaaaatatgt accatttggt ggaccagctg gcggtgacgg tggtaaaggt
     6061 gcttcagtcg tatttgaagt ggatgaaggt ttaagaacgt tattagattt tagatatcaa
     6121 cgtcatttta aagcaagcaa aggtgaaaat ggccaaagta gtaatatgca tggtaaaaat
     6181 gcggaagatt tagtattaaa agttccacct ggtacaatta ttaaaaatgt tgaaacagac
     6241 gaagtgttag cagatcttgt tgaagatggt caaagagctg tagtagcgaa gggcggtcga
     6301 ggtggccgag gtaattcacg ttttgcaaca cctagaaacc ctgcacctga cttcagtgaa
     6361 aaaggtgaac caggtgagga attagatgta tctttagaat tgaaattatt agctgatgta
     6421 ggattagtag gtttccctag tgtgggtaaa tcgactttat tatctatcgt ttcaaaagct
     6481 aagcctaaaa ttggggcata tcattttaca acgattaaac caaatctagg tgttgtttca
     6541 acgcctgatc aacgtagttt tgttatggca gatttaccag gtttaattga aggtgcatct
     6601 gatggcgttg gattaggaca tcaattttta agacatgtag agagaacaaa agttattgtt
     6661 cacatgattg atatgagcgg ttctgaaggt agagaaccta ttgaagatta taaagtcatt
     6721 aatcaagaat tagctgcgta cgagcaacgt ttagaagata gacctcaaat cgtagtagct
     6781 aacaagatgg atttacctga atcacaagat aatttaaact tgtttaaaga agaaattggc
     6841 gaagatgtgc cagttattcc agtttcaaca ataacgcgtg ataatattga tcaattatta
     6901 tatgcaatag cagataaatt agaagaatat aaagatgttg acttcacagt tgaagaagag
     6961 gagtcagttg gcattaaccg agtattatat aaacatacac cgtcacaaga taaatttaca
     7021 atttcaagag atgatgatgg tgcttatgtg gtaagtggta atgctattga aagaatgttt
     7081 aaaatgactg actttaacag tgatccagca gtacgtcgat ttgctcgtca aatgcgttcg
     7141 atgggtattg atgatgcgct tagagaacgt ggttgtaaaa atggtgatat cgttagaatt
     7201 cttggcggag aatttgaatt cgttgaatag gagcgaaaca tgatggacaa taaagattat
     7261 aaaaagtttt atttaattag agaagatgtc ttgcctgaat ccgtggttaa aacattgaag
     7321 attaaagatg ccttaaaaag tgatccgaca ttgtccattt atgatgccgt taaacagttt
     7381 gatctatcta gaagtgcttt ttataaatat agagaaacga tatttccagt agacgataaa
     7441 atgcttgacc atcgagaatt tacattaatt ttatatgtaa ctgatattgt tggtatgttg
     7501 gcacgtgtac tagatgttat atcaaagtta gaactatctg tattaacgat tcatcaaagt
     7561 attccaatgg aagaaaaagc aacaataaca ttatcactga atgctaaatc taaagaaact
     7621 tcagtagaag atgttattgg cgctttgaga aatttagatt atgtatcaaa agtagaatta
     7681 attagtatga gtatgtaagg atgtgcctat aatgtacgcg tatgtcaaag gtaagttaac
     7741 acatttatat cctacacacg tagttgttga aactgctggt gttggttatg aaattcaaac
     7801 accaaattct tatcgttttc aaaagcatct agatcatgaa gttttaattc atacatcttt
     7861 aattgttcgt gaagatgcac aattattgta tggatttagt agtgaagaag agaaagatat
     7921 gttcttgagt ttaattaaag ttactggtat tggtccgaaa tcagctttag ctattttagc
     7981 gacaagtacg cctaatgaag taaaacgtgc cattgaaaat gaaaatgata cgtatttaac
     8041 taaattccca ggaattggta agaaaacggc aagacagatt gtcttagatt taaaaggtaa
     8101 agtgaaaatt actgaagaag atagcgattc attattacaa gtagacgcta cttcgacggt
     8161 gcaagatcaa ttcgtgcaag aagcaatgtt agcgttagaa gcattaggtt attctaaacg
     8221 agagcttgca aaagttgaga aaacgttaaa taaaaataaa tatgactcag ttgatgaagc
     8281 tgttaaggca ggtcttcaat tagttgtatc ttaattttaa atagattaat aggggaagtg
     8341 ttgtcatgaa tgagcgtatg gttgatcaat caatgcatag tgaagaaact gatttcgaat
     8401 tgtcgcttag acctacgaga ttacgacaat atattggtca aaattcaata aaaagtaatt
     8461 tagaagtatt tattaaagcg gctaaacttc gtcatgaacc attagatcat gtattgcttt
     8521 ttggcccccc tggattaggt aagacaacat tatctaatat cattgccaat gaaatggaag
     8581 ttaatatacg tacagtatca gggccttcat tagaaagacc tggtgatttg gctgcaattt
     8641 tatcaggact tcaacctgga gatgttttgt ttattgatga aatacacaga ctgagtagtg
     8701 ttgttgaaga agtgttatac cctgcaatgg aagatttctt tttagatatt atcattggta
     8761 aaggcgatga ggctagaagt atccgtatcg acttacctcc attcactttg gtaggtgcaa
     8821 caacgcgagc tggcagctta acaggtccac taagggatcg atttggtgtg cacttaagat
     8881 tagaatatta taacgaatca gatttaaaag aaatcattat tagaacagct gaggttttag
     8941 gcacaggtat tgatgaagaa agtgccattg aacttgctaa acgttctaga gggactccaa
     9001 gagtagcaaa tcgactattg aagcgggtaa gagacttcca gcaagtgaat gaagatgaac
     9061 aaatatacat tgaaacaacg aagcacgcat taggtttact tcaagttgat caacacggac
     9121 tagattacat tgatcataaa atgatgaact gtattattaa gcagtataat ggcggacctg
     9181 ttggtttaga tacgattgcc gtaacaattg gtgaagaacg tattacaatt gaggacgttt
     9241 atgagccatt tcttattcag aaaggctttt tagaacgtac gccacgtggc agaaaagcaa
     9301 caccattagc ttatgaacat tttgcaaagt cgaatgagga gagagaataa cgtgaatatt
     9361 gaagaatttg actatgactt accagaatca ttaattgctc aaacgccttt aaaagatcgt
     9421 gatcatagtc gtttattagt catggataga gaaactggtg aaatgaaaca tttacatttc
     9481 aaagatatca ttgagtattt tagacctggt gatacattag tgcttaacga tacgcgagta
     9541 atgccagcta gactttttgg tttaaaagaa gaaactggtg caaaagttga aatgttaatg
     9601 ttaactcaaa ttgaaggtaa tgattgggaa gtcttactga aaccagctaa gcgtattaaa
     9661 gttggtaata aattgaattt tggtaatggc aaaattatag ctgaatgcat aaaagaaatg
     9721 gatcaaggtg gacgcatcat gcgtttacat tatgaaggta ttttacaaga aagattagat
     9781 gaattagggg aaatgccact gccaccatac atcaaagaac gtttagatga tccagatcgt
     9841 tatcaaacag tttacgctaa agaaagtggt tcagcggcag caccaacagc aggattacat
     9901 tttactgatg agttattaat tgaaattaaa aataaaggtg ttaatatcgc atttgttaca
     9961 ttacatgttg ggttaggtac gtttagaccg gtgagcgtag acgatgtgaa tgaccacgaa
    10021 atgcatagtg aatattatca aatgacacaa gaaacagctg atttattaaa tgatactaag
    10081 tccaaaggac atcgcattat atcagttggt acaacttcaa cacgtacact tgaaacaatt
    10141 cgacgcgatc atgataaatt tgttgaaacg agtggctgga ctaatatatt tatttatcca
    10201 ggatttgatt ttaaagcaat tgatggccag attactaatt ttcatttacc aaaatcaaca
    10261 ttagttatgc tagtatcagc gtttagtagt cgtgaaaatg ttctgaatgc ttataaaacg
    10321 gcagtaaatt tagaatatag attctttagt tttggcgatg caatgttaat tatataaaaa
    10381 gaatgtgagg attttgaata tgcctgcagt aacatacgaa cacattaaaa cttgtaaaca
    10441 atcaggtgcg cgtttaggta tcgtgcacac accacacggt tcatttgaaa cacctatgtt
    10501 tatgccagtt ggtactaaag caaccgttaa aacaatgagt ccagaagagt taagacaaat
    10561 tgaagcaaaa atcattttgg gcaacacata tcatttgtgg ttacaacccg gaaatgatat
    10621 tatcaaacac gctgggggat tacataaatt catgaattgg gatggtccga ttcttacaga
    10681 ttcaggcggt ttccaagtgt ttagtttaag taatttacgt aaaattacag aagaaggcgt
    10741 ggaatttaga catcatacta atgggtctaa attatttttg agtcctgaga aatcaatgca
    10801 aattcaaaat gatttaggat ctgatattat gatggcattt gatgaatgtc caccgatgcc
    10861 tgctgaatat gattatgtaa aaaaatctat tgaacgtaca acacgttggg cgaaaagatg
    10921 tctagatgca caccaaagac ctgaagatca agcattgttc ggcattatac aaggtggcga
    10981 atatgaagat ttaagagaac aaagtgcaaa ggatttagta gaattagatt ttcctggtta
    11041 tgcaatcggc ggtttgtcag ttggtgaacc taaaccggtt atgtataaaa tggttgaaca
    11101 tacagagcag tttatgccta aagataaacc aagatattta atgggtgtag gatctccaga
    11161 tgcgttaatc gaatgtagta ttcgcggcat ggatatgttt gattgtgtct taccgacacg
    11221 tattgccaga aatggtactt gtatgacatc gcaaggtcgt ttagttatta aaaatgcaaa
    11281 atttgcagat gatttaagac cgttagatga gaattgtgac tgttatacat gtcaaaacta
    11341 ttcaagagcg tatatacgtc atttaatcaa ggcagaggaa acttttggta ttcgtcttac
    11401 tactattcat aatttacatt ttctgctaaa attaatggaa gatataagac aagccattcg
    11461 agaagatcgt cttttagatt tcaaagaaga attcttcgag caatatggat taaatgttga
    11521 gaacccaaaa aacttttaag caagaggagc gtataaaatg caattttcat tactaatata
    11581 tatagtcgta atttttgcgg ttatgtattt cttgatgatc agaccacaac aaaaacgtgc
    11641 gaaacagcat cgtgagttga ttaataacat tcaatctggt caaagaatta caactattgg
    11701 tggtattaaa ggtactgtta aagcagtaga tgaaacaact gttgttatta cagttaatgg
    11761 tcatggtact gaattaactt tcgaaaaacc tgctattaaa caagttgacc cttcataatg
    11821 agaagtggta ttaaaaagga tgaaacgaat agcgtttcat cctttttaag ttataagtag
    11881 tatgtagaat agcttatttt tattccaaaa aaagaaattg tgatacataa taattgttaa
    11941 atttaaatta aatatatcta tgactatttt tactccctgc tctagtatgt taatatgtat
    12001 aaggctaata aattttgtaa gaaagattta acttgcgtta taaaatagta ttcaagtatt
    12061 ctaaatacat acgtaaataa tgaggtgttc atgtgaagaa aagtagtaga ataattgcgt
    12121 tcttgctttt agtcgtattg ttgtttgccg gaatggctgc aacttataaa agtgttataa
    12181 agaatgtcaa tttaggactt gatttgcaag gtggatttga ggtgctttat caagtcgatc
    12241 ctttaaataa aggcgacaaa atagataaaa aagccttaca atcaacagcg caaacattag
    12301 aaaatcgtgt taacgtttta ggtgtttcgg aacctaaaat tcaagttgaa gaacctaata
    12361 gaattagagt acaacttgct ggtgtaacag accagaatga agcacgtaaa attttatctt
    12421 ctcaagctaa tttaacaatt cgcgatgcag aagataaagt aaaattaagc ggttctgata
    12481 ttaaacaggg atctgccaaa caagaattta aacaggaaac aaaccagcct acagttactt
    12541 ttaaagtaaa agacaaaaac aaatttaaaa aagtaacgga agaaatctct aagaaacgtg
    12601 ataatgtcat ggttgtttgg ttagatttca aaaaaggtga cagctataaa aaagaagcac
    12661 aaaagaaaaa ccctaaattt atttctgcag caagtgttga tcaaccaatc aattctgata
    12721 gcgtagaaat ctcgggtgga tttaaaggac aagaaggcgt taaaaaagca aaacaaatcg
    12781 ccgaattgtt aaatgcaggt tctttaccag ttgacttgaa agaaatttat tcaaactcag
    12841 taggtgccca atttggtcaa gatgcattag ataaaactgt gtttgcatcg tttattggtg
    12901 tagcattaat ttatttattc atgcttggat tctaccgatt acctggttta gtagcgatta
    12961 ttgcattgac aacttatatc tatctaacgt tagtagcatt taatttcatt tccggggttc
    13021 taactttacc aggattagcg gcgttggtat taggtgtagg tatggctgta gatgccaata
    13081 ttatcatgta tgagcgtatt aaggatgaac ttcgaatagg tagaacgata aagcaagcct
    13141 tttctaaagc aaacaaaagt tcattcctaa caatttttga ttctaactta acaacagtta
    13201 tcgccgcagc agtattattc ttcttcggtg aaagttcagt taaaggtttc gcgacaatgt
    13261 tattattagg tattctaatg atctttgtta cagccgtgtt cttatcaaga ttcttattat
    13321 cattacttgt ttcatcaaat atattcaaaa atcaattttg gttatttggt gttaaaaaga
    13381 ataaacgaca tgatattaat gaaggtgtag atgttcatga ccttaaaact tcattcgaga
    13441 aatggaattt tgttaaatta gctaagccat taattggagt aagtatttta attgtagttg
    13501 tcggtttagt tattctttat atcttcaagt taaacttagg aattgatttc tcaagtggta
    13561 cacgcgtaga tttccaatct aaacaagcaa ttactcaaca aaaggtagag caagttgtaa
    13621 aagatagtgg acttaaagcg gatcaaattc agatcaatgg taaagacaat aaagtagcaa
    13681 ctgttcaatt taaagacgat ttaacgcgtg ctcaagataa taaattgagt gataatatta
    13741 aatcgaaatt cggtgacaca cctcaaatta acactgtatc accaattatt ggacaggaat
    13801 tagcaaaaaa tgcaatgctt gcattaatct atgcatcgat aggtatcatc atctatgtat
    13861 cattacgatt tgaatggcgc atgggtcttt catctgtatt ggcattatta catgatgtat
    13921 tcattatagt agcgattttc agtttattta gaattgaagt agatttaaca tttatcgccg
    13981 ctgtattaac aattgtcggt tattcaatta atgatacaat cgtaacgttt gaccgtgtac
    14041 gtgaaaactt acaaaaggtt aaagtgatta cgacaacaga acaaattgat gatatcgtta
    14101 atagatcaat tagacagaca atgacacgtt caattaatac agtattaaca gttattgtag
    14161 tagtagttgc tatactattc ttcggtgctc ctacgatatt caactttact ttagcattat
    14221 ttatcggatt gatttctggt gtattctctt caatcttcat tgccgttccg ctatggggaa
    14281 taatgaaaaa acgtcagttg aaaaaatcgc cgaaacacaa attagttgta tataaagaaa
    14341 agaaatcgaa cgatgaaaag attttagttt aaaatgaatt aagcggtatg tgaaacaata
    14401 aagagttcac ataccgcttt ttactttatt taaatttaac aaaatatata taaataagtt
    14461 cccgcattca tttttaaaaa atggtataat taaagtcttg attatttaga taagtgcttt
    14521 ttatttaaag taattttaga tataatgtct tttggaaatg aggcgtaaat atatatgatt
    14581 aaaccgaagt ataagtggaa attaactaaa ccagctgaat acataagtga tgaattaaca
    14641 tcaaaattaa agttaacgcc aatcgtaaaa aaaatattag aaagtaaatc gattattgat
    14701 gaacaagcaa tagaatccat catttcagat acagatatca atcatgatgc actgcaattg
    14761 agcgatatga ctaagactat tgaaagaatt aaaagagcga tagcgaatga tgagaaaatc
    14821 ctagtttatg gtgattacga tgccgacggt gttaccgcaa ctacaatttt agtgataaca
    14881 ttacaacttt taggtgcaca agtaggatgg catattccta accgctttac cgaaggatat
    14941 ggaccaaacg aactagcatt tagaaatgct catgatgaag gcataacatt aatcataact
    15001 gttgataatg gcatacaagg tcataatgaa attaaaatgg ttcaagattt aggagttgat
    15061 gtcattgtaa ctgatcatca tgaaattggt agtacattac cagaagcata tgcaatcgta
    15121 catccaatgc atccatcgtt taattatccg ttccaacaat tgtgtggtgc aggtgttgca
    15181 tacaaattag cacaagcatt gattgaaaat gtacctgatt attttaaagc attagttgcg
    15241 ataggtacca ttgcggattt agtatcctta actgatgaaa atcgatcatt agtgaaacaa
    15301 ggattaaaag tcttaaatga tcaatgtccg acctctgtta aagcattgct aaaagaagcg
    15361 ggatacaatg acaatattga tgaagaaaca attggtttta ttattggacc tagacttaat
    15421 gctgtaggtc gtttagatga cgcatcatta gcttgcgaat tgttgatgac tgacgtcgaa
    15481 gaagaagctg catttttagc agaacaagtt gaacatttta accgtgaaag aaaagatatt
    15541 gtagcaacta tcacagaaga agcaatggct atggcagaaa cgaaagttaa aaagggagat
    15601 ttatttttac ttttagcaaa agaaaattgg catgaaggtg tcttaggtat tgttgcttcg
    15661 aaaattgtcg aaacatttgc tttgccaaca ttaattttaa atattgatag ggagcaaaat
    15721 catgcaaaag gctccgccag atcaattgat caagtgtcga tgtttgaaat actatcagca
    15781 catcaagagt taatcgctaa atttggcggc catcatatgg ctgcaggtat gacgatggat
    15841 attgaaaata tagaatcgtt agctgaaggt ttaaataaat ggatgaaaga attatctaaa
    15901 acaacttcgt tagatcccgt aaagcaagta gatgtattat taacagaaaa tgatataacg
    15961 attaaaaata ttcgtgatat gaatcgttta agacccttcg gaacagattt ttctagacct
    16021 atttttgaaa tggatgattt atcagtttct tcagttaagg cgataggtca acaaaaaaat
    16081 catctaaaat tgacacttgg tgaaagtaat atcgccgcgc tattttggca aaatggacac
    16141 ttagaacctg agttacaaga tgaacagcca attaatatat taggatctgt tcaaatcaac
    16201 gaatggaatg gtaatcaatc accgcaaata attattcaag atattgcgat gaatgaacag
    16261 caaatattag attatagaag taagcgaaaa agtttacctt ttacagaaaa tgatgaaaat
    16321 attgtcgtgc ttattcatcc taaaagtgat aaagtaaatg cgaatgaata ttattatggt
    16381 gaagaaatta aacaacaaac tgataaagta gtattaagag atttaccaac gtcaatggaa
    16441 gacttgtcta attccttgca acaactgcaa ttttctcaac tttatatagt tttgcaacat
    16501 aatcattcga tttacttcga tggtatacct aatatggata tttttaaaaa gtgttataaa
    16561 gcattaataa ctaaacaaga aacaaatatc cagaaagagg gtatgttatt gtgtcaacat
    16621 ttaagtgtga aaccagatac acttaaattc atgttgaaag ttttcttaga cttaaaattt
    16681 gtaacacaag aagatggttt aattcgaatc aatcaacaac ctgataaaag atcgattgat
    16741 tccagcaaag tatatcaatt aagacaacaa cgtatggatg ttgaaaagca attattatat
    16801 caagattttt cagaaataaa aaattggata aagtcacaat tgtcgtgagc aatttaggag
    16861 gaaatattaa tggatttaaa gcaatacgta tcagaagttc aagattggcc gaaaccaggt
    16921 gttagtttca aggatattac tacaattatg gataatggtg aagcatatgg ctatgcaaca
    16981 gataaaattg tagaatacgc aaaagacaga gatgttgata tcgttgtagg acctgaagcg
    17041 cgtggcttta tcattggctg tcctgtagct tattcaatgg ggattggctt tgcacctgtt
    17101 agaaaagaag ggaaattacc tcgtgaagtc attcgttatg agtatgacct agaatatggt
    17161 acaaatgttt taacaatgca caaagatgca attaaaccag gtcaacgtgt gttaattaca
    17221 gatgatttat tagctactgg tggtacgatt gaagcagcaa taaaattagt tgaaaaatta
    17281 ggcggtatcg tagtaggtat tgcatttata attgaattga aatatttaaa tggtattgaa
    17341 aaaattaaag attacgatgt tatgagttta atctcatacg acgaataata aataatataa
    17401 ttttatcaaa tgaaatcctt catcaaatgt ataagaacca atgacttaat taaaaaagtt
    17461 gtttaagttt tcttaacatg agatgttagg attttttatt tactgaaaat g
//
LOCUS       DAKTIO010000044        17348 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.044, whole genome shotgun sequence.
ACCESSION   DAKTIO010000044 DAKTIO010000000
VERSION     DAKTIO010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17348
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.044"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            100..465
                     /locus_tag="O0293_001092"
     CDS             100..465
                     /locus_tag="O0293_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HCY0279984.1"
                     /translation="MKKKKNAIKVIIILFISLIVAVAFFFGLKTYQGHKNIQLIDSYL
                     EEKNLKDKIKSEKTEYSAKKGLFYKEVTFKDEPGVTYVVQPISTNKGLFVEGFDTETK
                     KSLKTAKHKYFNQNYKPSK"
     gene            647..1924
                     /locus_tag="O0293_001093"
     CDS             647..1924
                     /locus_tag="O0293_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCY0279985.1"
                     /translation="MALFKRKISLPMQVVIALVLGVVVGLLLYGQENVANYIKPFGDV
                     FLNLIKMIVIPVVFCSLALSISNVGESKTVGRYGWKTILYFEIITTIAIGLGIIFGNL
                     FKPGAGLDPTKLPKGDISKYQSTAHAAEQSTYGNHFIDTIVHIIPTNFFEALNKGELL
                     PIIFFAVFFGLGLAAVGKKAEPVKEFLSGSLEAVFWMINKILKLAPLGVFAFICTTII
                     TFGASALLPLLKLVLVVVFAMVFFVFAILGLVAWMCGINIMNIIRILKSELLLAFSTS
                     SSEAVLPVMMKKMENFGSPKEITSFVIPIGYTFNLDGSALYQSIAALFVAQMYGMHLT
                     LSEQIVLMLTLMITSKGMAAVPGTSIVVLLTTLGAMGLPAQGLALIIGVDRILDMVRT
                     CVNVIGNALSTIVIAKWENVYDKAKGQEYLKSI"
     gene            complement(2328..2684)
                     /locus_tag="O0293_001094"
     CDS             complement(2328..2684)
                     /locus_tag="O0293_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HCY0279986.1"
                     /translation="MKKKKGFGLGISLIAIMLIVCIVLVIMMMTGGKKDTYYGIMKDN
                     TTIEKMISEKDESIEKNVKLPSDSDVKVKKGDFVIVYKLADSDKIVKVKKVDHDDVPH
                     GLMMKIHDMGKMHMKH"
     gene            complement(2949..3371)
                     /locus_tag="O0293_001095"
     CDS             complement(2949..3371)
                     /locus_tag="O0293_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HCY0279987.1"
                     /translation="MSNSQAIQAIENVLATSKVGVLSTAYNNKPNSRYMVFYNDGLTL
                     YTKTNIHSAKVKEIKDNPAAYVLLGYNDTTNRSFVEMEATIEIVTEQEVIDWLWETQD
                     KSFFSSKEDPELCVLRVVPQSIKLMNDKSLDTPIKIDL"
     gene            complement(3442..5547)
                     /gene="rsp"
                     /locus_tag="O0293_001096"
     CDS             complement(3442..5547)
                     /gene="rsp"
                     /locus_tag="O0293_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator Rsp"
                     /protein_id="HCY0279988.1"
                     /translation="MTCQLKIHHTLSTIPSRNINNIMVLFSLTSKLNITINGETKDVS
                     NYIILINHGDIYNINHGENIIELMIPVFYFYQQDDDFFNGYLDRHLLQSSNYIKSLIA
                     DLISTPTSSSLMGKNIGQSIIDTLLKEAFIRIDHEYLPNIALSNPVFIDCVNYIHDNI
                     DAHLSLKDIAMHCNISESYCSNLFVRYLSMNFKDYFTSIKLVNAINLLLSTKHSITTV
                     SELAGFNSHTNFANQFKNYLHFSPKQFRSLVSKITEPPQIHFQQDNVSQFTELISTID
                     LTAQLATNTTDIHIDDFNPKDRSQRAKVFVRFSNFNELFQFIFNEYYDINFEHLPKPV
                     VFIDDIHDIEISQTNYNLLNRCFEKLFEKNIGLAIAIKSTQQFETMKQLILTFLQGNQ
                     DYKTSKKLVKFMLVFCSNSMTAEEIHLCHLKIKNKNKEIKYSVTVDGFLETYSTVEQV
                     YDVMQRLKFHYYFIDIENSKTATHLITKNQHYHQTDTHFEQYKKFILDSGISSTQFVY
                     NNLSVSGFKYTNDGKNPIQLSDIVYHLIALLRYGGGISYQLLDDHSNYISLYNKYGSP
                     LPLMHLYKMFRPFVNEDIEITNNYVLSRKDNNYHFLLFNKINDRYMSDVKQDFIFHNE
                     LPQDSLMIIKTLNHEHGSIQHLLPISDQLVYIEKEILDELDKTNYPKTELAVQEETGR
                     TFELKLNHDEVKYICFKPS"
     gene            complement(7035..7418)
                     /locus_tag="O0293_001097"
     CDS             complement(7035..7418)
                     /locus_tag="O0293_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCY0279989.1"
                     /translation="MKERGYIEQLWREEKYHVLLHSQQSYQMIRNALKTDLSLHQVQQ
                     MIDVALLIEPSIGSVCNAFDHMWGYLKKCANEEERQQSKLLKADFINGKIDTQTLLDF
                     LAELANKYDVQYLLQSRVLNTKRKR"
     gene            7601..9043
                     /locus_tag="O0293_001098"
     CDS             7601..9043
                     /locus_tag="O0293_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HCY0279990.1"
                     /translation="MNYKQSAEEILNAIGGEENLDAMAHCATRLRLVLNDESLVNEEA
                     LNNMDVVKGTFSTGGQYQIIIGSGTVNKVFSELEKLTGKEASTTSEVKAQSAKNMNPL
                     QRFVKMLSDIFVPIIPAIVAGGLLMGLNNILTAKDLFFSGKSLIDVYSQFAGLAEMIN
                     VFANAPFTLLPILIGFSAAKRFGGNPFLGAALGMILVHPSLMSAYDFPKAVEAGKAIP
                     YWDVFGLHINQVGYQGQVLPMLVAAYILASIEKGLRKVIPTVLDNLLTPLLSIFITAF
                     LTFSFVGPITRQLGYWLSDGLTWLYEFGGAIGGLIFGLLYAPIVITGMHHSFIAVETT
                     LIADATKTGGSFIFPIATMSNVAQGGAAIAAFFIIKQNKKLKGVASAAGISALLGITE
                     PAMFGVNLKLRYPFIGAIVGSGIGSAYIAFFKVKAIALGTAGLPGFISINPVHAGWLH
                     YFVGMTISFIIAITVTLILSKRKANKEVVE"
     gene            complement(9129..10073)
                     /locus_tag="O0293_001099"
     CDS             complement(9129..10073)
                     /locus_tag="O0293_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCY0279991.1"
                     /translation="MITSFRHSEDIDKHIIKTPLDHTASWINVVEPDREEIENLMEQY
                     NIPEDFIRDPLDSEESSRIEYDEDTGYSLIIIDLPIVNSTNRSVLSFVTIPLGIIIGN
                     GIIVTVCDAENEFLENLPKRDINLKFHSRFALEILTTIADHYNRNLRLLNKSRIRIEK
                     ELKNNITNKQLFKLMEVEKSLVYFLAALKGNDTIIKKLFRLPAIKRFEEDEELLEDLI
                     IENNQAIETTELHQRILESITTSYASLLSNDMNTIMKTLTLFTVLLTLPTLVFSFFGM
                     NVPLPIDDHSYISWIIVVGISLILVVIVSIFLWRKQKL"
     gene            complement(10119..10742)
                     /locus_tag="O0293_001100"
     CDS             complement(10119..10742)
                     /locus_tag="O0293_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCY0279992.1"
                     /translation="MKEDRRIRKTKSSIKQAFTKLLQEKDLEKITIRDITTRADINRG
                     TFYLHYEDKYMLLADMEDEYISELTTYTQFDLLRGSSIEDIANTFVNNILKNIFQHIH
                     DNLEFYHTILQLERTSQLELKINEHIKNNMQRYISINHSIGGVPEMYFYSYVSGATIS
                     IIKYWVMDKQPISVDELAKHVHNIIFNGPLRIMAENRLHKSNLDSLT"
     gene            10903..12180
                     /locus_tag="O0293_001101"
     CDS             10903..12180
                     /locus_tag="O0293_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCY0279993.1"
                     /translation="MNIFKNKLLWIAPIATMIILVIFSLAFYPAYNPKPKDLPIGILN
                     EDKGTTIQDKNVNIGKKLEDKLLDSDSNKIKWVKVDSEKDLEKDLKDQKIFGVAIIDK
                     DFSKDAMSKTQKVVMDSKKEEMQQKVASGEIPPQVVQQMKQKMGNQQVEVKQAKFKTI
                     VSEGSSLQGSQIASAVLTGMGDNINAQITKQSLETLTSQNVKVNAADINGLTNPVKVD
                     NEKLNKVKDHQAGGNAPFLMFMPIWIGSIVTSILLFFAFRTSNNIVVQHRIIASIGQM
                     IFAVVAAFAGSFVYIYFMQGVQRFDFDHPNRIAIFVAFAILGFVGLILGVMVWLGMKS
                     VPIFFILMFFSMQLVTLPKQMLPESYQKYVYDWNPFTHYATSVRELLYLNHHIELNST
                     MWMFIGFMIFGAVSSLVSAIVRKHSTKRTEVPS"
     gene            12387..12818
                     /locus_tag="O0293_001102"
     CDS             12387..12818
                     /locus_tag="O0293_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2871 domain-containing protein"
                     /protein_id="HCY0279994.1"
                     /translation="MRRLLYSFLFYMIIGLFSGFFYRELTKAYDFTGTTQLSLVHTHT
                     LILGMFMFLILLPLEKLFKLSSYYLFNWFFYVYNIGVIVTIGMMVTKGFFQVTGKSYS
                     PEAFAGFAGIGHTGMLAGLLLLFFLLRQAILKEPRDESFKK"
     gene            12923..13039
                     /locus_tag="O0293_001103"
     CDS             12923..13039
                     /locus_tag="O0293_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0279995.1"
                     /translation="MANYAIYFNNININSIMALNDNNYQFEHVNERNEYRES"
     gene            13050..14084
                     /locus_tag="O0293_001104"
     CDS             13050..14084
                     /locus_tag="O0293_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0279996.1"
                     /translation="MKDVTIIGGGPSGLYASFYAGLRDMSVRLIDVQSELGGKMRIYP
                     EKIIWDIGGIAPKPCHEILKDTIKQGLYFKPEVHLNERVVDIRKKAERHFEVETEAGE
                     IYTSKAVIIAIGAGIINPKQLDVKGVERYQLTNLHYVVQSYRRFKDKDVLISGGGNTA
                     LDWAHDIAKIAKSVTVVYRKEDVSGHEAMKTLVTDLNVKLCPKTRIKYLVGNDDETHI
                     SEVVLEHVESGDRHTVKFDDVIISHGFDRCNTLLSETSSKLDMHDDCRVKGFGNTTTS
                     IPGIYACGDIVYHDAKSHLIASAFSDGANAANLAKTYIQPDANAEGYVSSHHEVFKEA
                     NKTIVNKHLY"
     gene            14120..14521
                     /locus_tag="O0293_001105"
     CDS             14120..14521
                     /locus_tag="O0293_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0279997.1"
                     /translation="MVKVTYDIPTCEDYCALRINAGMSPKTREAAEKGLPNALFTVTL
                     YDKDRLIGMGRVIGDGGTVFQIVDIAVLKSYQGQGYGSLIMEHIMQYIKGVAVESTYV
                     SLIADYPADKLYTKFGFIPTEPDSGGMYIKY"
     gene            complement(14567..15571)
                     /locus_tag="O0293_001106"
     CDS             complement(14567..15571)
                     /locus_tag="O0293_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCY0279998.1"
                     /translation="MIETFKAFVIDKDESGKVTPTFKQLSPTDLPKGDVLIKVHYSGI
                     NYKDALATQDHNAVVKSYPMIPGIDLAGTIVESEAPGFEKGEQVIVTSYDLGVSHYGG
                     FSEYARVKSEWIIKLPDTLTLEESMIYGTAGYTAGLAIERLEKVGMNIEDGPVLVRGA
                     SGGVGTLAVLMLNELGYKVIASTGKQDVSDQLLELGAKEVIDRLPVEDDHKKPLASST
                     WQACVDPVGGEGINYVTKRLNHSGSIAVIGMTAGNTYTNSVFPHILRGVNILGIDSVF
                     TAMKLRQRVWRRLAKDLMPENLHEIKQVITFDELPEQLNKVIKHENKGRIVIDFGVDK
                     "
     gene            15762..16277
                     /locus_tag="O0293_001107"
     CDS             15762..16277
                     /locus_tag="O0293_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0279999.1"
                     /translation="MAGIIKEISEQNASELVELATRTFYDTFGSYYDDKDFDQFFKDN
                     YTVEKFTQEINHVDSFHYFYQEDGANVGYIKMNINSAQTEEMGETYLEVQRIYFLKDF
                     QGGGRGSQLIELAEKIAQEHNKHKIWLGVWEHNPRAQAFYKRHGFKVVGEHHFQTGDV
                     TDTDLIMEKEL"
     gene            16591..17220
                     /locus_tag="O0293_001108"
     CDS             16591..17220
                     /locus_tag="O0293_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280000.1"
                     /translation="MKRLVTGLLALSLFLAACGQDSDQQKDGNKEKDDKAKTEQQDKK
                     TNDSSKDKKDNKDDSKDVNKDNKDNSANDNQQQSNSNATNNDQNQTNNNQSSNNQANN
                     NQKSSYVAPYYGQNAAPVARQIYPFNGNKNQALQQLPNFQTALNAANNEANKFGSNNK
                     VYNDYSIEEHNGNYKYVFSFKDPNANGKYSIVTVDYTGQAMVTDPNYQQ"
BASE COUNT         6053 a         2485 c         2928 g         5882 t
ORIGIN      
        1 atattgagaa taaaaaaatt tgttacaatc tggtaaaaac tctttattaa ataaattgat
       61 tcgttcatac tgaatagata agcaaaaagg agttacataa tgaagaagaa aaaaaacgcg
      121 attaaagtta tcattatatt atttatttca ttaattgttg cagtagcatt tttctttggt
      181 ctgaaaacat atcaaggcca taagaatatt caactaattg acagttactt ggaagagaaa
      241 aatctaaaag ataaaattaa atcagaaaaa acagagtata gtgctaaaaa aggtctcttc
      301 tataaggaag ttacatttaa agatgaacct ggtgtaactt atgttgtgca accaatcagt
      361 actaataaag gattatttgt tgaaggcttt gatacagaga ctaagaaaag tttgaaaact
      421 gcaaagcata aatatttcaa tcaaaattat aaaccatcaa aatagtgaaa cgttaaactg
      481 gtgaagcagt ttagtgcctt caattttaat tatttgaggt aaacagtttt tgtatggaaa
      541 ttagtgattt ttgacatcta taaaagataa atattttaat tttttataga taattatcaa
      601 ttttctgata taattgagtg atgtcagatg aaggggagtt tgtactatgg ctctattcaa
      661 gagaaaaatt agcctaccta tgcaagttgt tattgcatta gtgttaggtg ttgtcgtagg
      721 acttttatta tatggacaag aaaacgtagc aaattacatt aaaccatttg gtgacgtatt
      781 tttaaattta attaaaatga tcgttatacc agttgtattt tgctcactag cgctttctat
      841 ttcgaacgtt ggggaatcga aaactgtagg gcgttatggc tggaaaacaa ttttatactt
      901 tgaaattatt acaacaatcg caataggttt agggattatc ttcggtaacc tatttaaacc
      961 aggtgctgga ttagacccaa caaaattacc taaaggtgat atttctaaat atcaatcaac
     1021 tgcacatgca gcagaacaat ctacatatgg aaatcatttt attgatacca ttgtacatat
     1081 tattccgaca aacttttttg aagctttaaa taagggtgaa ttattaccta ttatcttctt
     1141 cgcagtattc tttggattag gattagctgc tgtaggtaaa aaagcagaac cagttaaaga
     1201 atttttaagc ggatcgcttg aagctgtgtt ctggatgatt aataaaattt taaaattagc
     1261 accacttgga gtgtttgcat tcatttgtac tacaattatt acatttggtg catccgcatt
     1321 attaccacta ttaaaattag tattagttgt tgtctttgca atggtgttct ttgtattcgc
     1381 tatactagga ctagttgcat ggatgtgtgg tattaatatc atgaatatta ttagaatctt
     1441 gaaaagtgaa ttgcttttag cattttctac atcaagttcg gaagctgtac ttcctgtaat
     1501 gatgaagaaa atggaaaact tcggttctcc aaaagaaatt acttcttttg ttataccaat
     1561 tggttatacg tttaacttag atggatcagc actttatcaa tctattgcag cattattcgt
     1621 tgcacagatg tatggaatgc acttaacatt atcagagcaa attgtgttga tgttaacatt
     1681 aatgattacg tctaaaggta tggcggcagt accaggaact tcaatcgttg ttttattaac
     1741 aacattaggt gccatgggct taccggcaca aggtttagca ttaattattg gtgttgaccg
     1801 tatcttagat atggtacgta catgtgtaaa cgttattggt aatgcattat caacaatcgt
     1861 tatagctaaa tgggaaaacg tatatgacaa agcaaaaggt caagaatatt taaaatcaat
     1921 ttaaaaaata ctatctgaca tttaaagccc cttacaacct ttggttgtta ggggctttta
     1981 tgcatgcgct aaagcgcagg cgaacggaag cgtagtgacc tcaggttgtt agggactttt
     2041 atgcatgcgc taaagcgcag gcgaaccgaa gcgtagtgac ctcaggttgt taggggcttt
     2101 tatgcatgcg ctaaagcgca ggcgaaccgg agcgcagtga cctcaggttg ttaggggctt
     2161 ttatgcatgc gcaaagcgca ggcgaacggc aagtggagct attcaatatg aaagtatttc
     2221 aaatataaaa agcagcagta aaatgattcc aaattggaaa tcaacttacc gctgctttta
     2281 ctcgtatata ccttgatggc aactaaaatt tgtaatttaa attacaatta gtgtttcatg
     2341 tgcattttgc ccatgtcatg aattttcatc attaaaccat gtggtacatc gtcatggtca
     2401 acttttttaa ctttaacaat tttatctgaa tctgctaatt tataaacaat tacaaaatca
     2461 ccttttttaa ctttaacatc tgaatctgaa ggtaatttaa catttttttc aatactttca
     2521 tctttttcac taatcatttt ttcaatagta gtattatctt tcataattcc atagtatgta
     2581 tctttctttc cgccagtcat catcatgatt actaatacaa tacatacaat taacatgatg
     2641 gcgattaaac taataccaag accaaaacct tttttctttt tcatggttca tcaatctcct
     2701 taaatgtagt taattctgtt atacctaaaa gtgcaacagt ttgcaaatgc agtatttata
     2761 ttacataaaa ttaatttaaa aagacacacc ctaatcacgt taaatgtgaa taaagtgtgt
     2821 ctaaaaaaca atacattgca aattgaattg taaacatatt aaacacttta attatagcga
     2881 gttattttaa gtatttaaca ttgttatatc tagaattcat aaaagttatt tcctatatac
     2941 actttgtgtt ataaatcgat tttgataggt gtatctaatg atttatcatt cattagctta
     3001 atggattgcg gaactactct taaaacacat aactctggat cttcttttga actgaaaaag
     3061 cttttgtctt gtgtttccca tagccaatca atcacttctt gttctgtaac gatttcgatt
     3121 gtcgcttcca tctcaacgaa actacgatta gttgtgtcgt tatagcctaa caaaacatat
     3181 gctgctggat tatctttaat ttctttgacc ttagcagaat ggatattcgt tttagtatat
     3241 aaagtaagac catcattata aaagaccata tatctactat taggtttatt attatatgca
     3301 gttgataata caccaacttt tgacgttgct aacacgtttt caattgcttg aattgcttgt
     3361 gagttactca ttacaaatca tctccttatg atataaatag cctttaacgt aagaaactaa
     3421 acacaagagg actgttagta tttagcttgg tttaaagcaa atatatttaa cctcgtcgtg
     3481 atttaacttg agttcaaatg tacgaccagt ttcttcttga actgcaagct ccgttttagg
     3541 gtaattcgtt ttgtctaatt catctaaaat ttctttttct atataaacaa gttgatcgct
     3601 tattggaagc aaatgttgaa ttgaaccatg ttcatgattc aatgttttaa taatcatcaa
     3661 agagtcttga ggtaattcat tatggaaaat gaaatcttgt tttacgtctg acatatatcg
     3721 atcattaatt ttattgaata ataagaaatg gtagttatta tctttacgac tcaatacata
     3781 attatttgta atttcaatat cttcattaac aaaaggtcta aacattttat ataaatgcat
     3841 taatggtagg gggctaccat atttgttgta caaggaaata taatttgaat ggtcatctaa
     3901 taattgatag ctaatgccac cgccatatcg taataatgcg attaagtgat acactatgtc
     3961 agataattga attggattct taccatcatt agtatattta aatccgctta cagacaggtt
     4021 attatataca aattgtgttg atgatatacc tgaatctaaa ataaatttct tatactgttc
     4081 aaaatgagtg tcagtttgat gatagtgttg gtttttagtg ataagatgtg ttgcagtttt
     4141 tgaattttca atatcaataa agtaataatg gaatttaaga cgctgcatga catcataaac
     4201 ttgttccacg gttgaataag tttctaaaaa gccatcgact gtcacactat atttaatttc
     4261 tttatttttg tttttaattt taagatgaca taaatgaatt tcttctgctg tcatagaatt
     4321 agagcaaaat acgagcataa atttaactaa tttcttgctt gtcttatagt cttgattgcc
     4381 ttgtaaaaat gttagtatta attgcttcat cgtttcgaat tgttgtgttg atttaatagc
     4441 gattgctaaa cctatatttt tttcgaaaag cttttcaaaa catctattta acaggttgta
     4501 attcgtttgg ctaatctcaa tatcgtgaat atcgtcaatg aaaacgacag gttttggtaa
     4561 gtgctcaaag ttaatatcgt aatattcatt gaaaataaat tgaaatagtt cattgaaatt
     4621 actaaatcga acaaatactt tggcacgctg acttcgatct ttcggattaa aatcatctat
     4681 atgaatatct gttgtatttg tagccagttg agctgttaaa tcaattgttg aaattaactc
     4741 agtgaattgt gatacgttat cttgttggaa gtgtatttgt ggtggttcag taatcttgga
     4801 aacgagtgaa cggaactgtt taggactaaa atgcaagtaa ttcttaaatt gatttgcaaa
     4861 atttgtatga ctgttaaaac cggccaattc agaaacagtt gtaatagaat gttttgtaga
     4921 aagtaataaa ttgatggcgt taacaagttt aatacttgta aaataatctt taaaattcat
     4981 actcaaatat cgaacaaaaa gattagaaca atatgattca gagatgttgc aatgcatcgc
     5041 tatatctttt aaagacaagt gcgcatcaat attgtcatga atatagttta cacagtctat
     5101 gaacaccgga ttgcttaaag caatatttgg taaatattca tgatctattc ttataaaagc
     5161 ttctttaagt aaagtgtcta taatactttg acctatattt ttccccatta aagaagaact
     5221 tgttggtgta ctgattaaat cggcaattaa tgacttaata taattacttg attgtaataa
     5281 atgacgatct aaataaccat taaagaaatc atcatcttgt tgataaaaat aaaaaacggg
     5341 tatcattaat tcgattatat tttcgccgtg gttaatatta taaatatcac catggttaat
     5401 taagataata taattactta cgtctttggt ttcaccatta atcgtaatat tgagtttact
     5461 tgtaagtgaa aatagcacca taatattatt aatattacga cttggtattg tcgataatgt
     5521 atgatgtatt ttaagttggc atgtcatatt tctctctcct gcctaatata tattgaaatc
     5581 tatcatttaa atgtaaaaca tggaaatttt taaaatcatg gatagtatac ctattatagt
     5641 tgaatatttt tgaaaaggta atagtgaaaa ataaaagaaa ttaatgaaaa ctcctaggaa
     5701 aacgcttgtt tttatgcatt gcgaacaata taaaagtaaa attagaaaga tagtaagcaa
     5761 aatttggaaa ttttcgatag agtctaatta aattatttgg tgcataatta atatagattt
     5821 caaacctatg tatttcaaaa aacaatacat agccattttg aactaacttg ctaatctttt
     5881 attgatattc aactatgtta taaaaaaagc aaattgacca aaatggaagt ttgaatcaaa
     5941 aaacattaaa tgtacccatg acttaaattt tatcgccatt atttaagttt ggacatacac
     6001 ttttgtcatc aaacgctgaa aattagtacg attgaaatca cttcttgtta taaaaattct
     6061 tagtagccct gttacaaaat attcgctaca aattattcaa atcaacaaca ataaccatca
     6121 actaagaaat tttaaacaat ctactaaaaa ataaagcgtt taaaagttct gtttagaaaa
     6181 catcaagata agtataaaca tctagaaaag tgaacatcta agaaaatcca tctagttatt
     6241 gctgtaacaa ttagaataat catttgaaaa agtgcattag acaacgaaat tcaccatcaa
     6301 gataatgaag caacaagaaa cagcacacat caatgaatta acaccttaga ttcatcatga
     6361 gaaaatgcat ggcacaacta gatttaccat caagataatt cttaagcagc gaagaattaa
     6421 catctagaaa tgcataaaca tcttagattt tacatcaaga gatgcgataa ctataaacaa
     6481 ctagaaacgt caccaagata gtgcaacaac aaagaatcag catcttgaaa tcgcactaac
     6541 atctaagaat caacatcaag tattatatta acatcataga taacttcatc aagacaggca
     6601 tccacatcta agaaactcat ctagaatagc taacacctaa catcaaagaa tattcatcaa
     6661 gaacagtatc taacagaact ttttgactat atatgaatta attatgttac tagctaaatc
     6721 atctattatt aactttcaca ctcaatttct acatctaata tcaatttatt aacaatagaa
     6781 ttagcagcaa caagatatta cttaaaatta ctcccccgaa tttaaaatat aaacaatgta
     6841 ataattaagg gtataaattg atttatgctg taactaatta aatgtgtatg gtatcttcta
     6901 acatcatgaa acgcatttta atatagatat gtacatttaa accttgagag atagattata
     6961 gtaaacttta gtacaaagta tgaattgctg aactccaatg actactattt gtccgttaca
     7021 actaaagttt caatctatct ctttcttttt gtgtttaaaa cacgactttg tagtaaatat
     7081 tgaacatcat atttattagc aagttctgct aaaaaatcta ataaagtttg ggtgtcgatt
     7141 ttaccattaa taaaatcagc tttaagtagt tttgattgtt gtctttcttc ttcattcgca
     7201 cattttttta aatatcccca catatgatca aaagcattac atacacttcc tatactcggt
     7261 tcaattaaca aagcaacatc aatcatttgt tgaacttgat gaagtgataa atccgttttt
     7321 aaagcattcc ttatcatttg gtaagactgt tgactgtgta acaatacatg atatttttcc
     7381 tcacgccaaa gctgttcgat atagccacgt tctttcataa aattacctcc gataattaat
     7441 gttaatatac atgcatgtag tatagcaata aaaatgaatg cgctatcaaa aatggtgtag
     7501 gtgtttatat aaaatgataa tagagtgttg caaatatttt tggaaccggt tacaataaag
     7561 tggaataaat ggaaccggtt ctaattagga ggctaaatat atgaattata agcaatccgc
     7621 agaagaaatt ttgaacgcga taggcggaga agagaattta gatgcaatgg cgcattgtgc
     7681 aacgagacta cgattagttt taaatgatga aagtttagta aatgaagagg cgctaaacaa
     7741 tatggatgta gttaaaggga cgttttctac tgggggacaa taccaaatta ttattgggtc
     7801 tggtacagtc aataaagtat ttagtgaact ggaaaaatta actggaaaag aagcatcaac
     7861 cacttcggaa gtcaaagcac aatctgctaa aaatatgaat ccgttacagc gatttgtaaa
     7921 aatgctttca gatatctttg ttccgattat accagccatc gttgctggtg gtttattaat
     7981 ggggttaaat aacattttga ctgcgaaaga tttattcttt tcaggtaaat cattgataga
     8041 tgtatatagt caatttgctg gattagctga aatgataaat gtttttgcga atgcaccatt
     8101 tacattatta ccaattttaa ttggatttag tgcagcaaaa cgctttggtg gcaatccatt
     8161 tttaggtgct gcattaggta tgatactagt tcatccatcg ctaatgagcg catacgattt
     8221 cccaaaagca gttgaagcag gtaaggctat tccatattgg gatgtttttg gtttgcatat
     8281 taatcaagta ggttatcaag gacaagtgtt acctatgctt gtagcagctt atatcttagc
     8341 ctcaattgaa aaagggttac gcaaagttat tccaacggtg ttagataatt tgttaacacc
     8401 attgttatct atttttataa cagcatttct aacattttca tttgtaggtc caatcactcg
     8461 acaattaggt tactggttat cagatggttt aacatggctt tatgaatttg gtggtgcaat
     8521 tggtggatta atattcggat tattgtatgc tccgattgtt attacaggta tgcatcatag
     8581 ctttatagct gtagaaacga cattaattgc agatgccact aaaacgggtg gatcatttat
     8641 attcccgatt gcgacaatgt ctaatgttgc acaaggtggt gcagcaattg cagcgttctt
     8701 tattattaaa caaaataaga agttaaaagg tgtggcatct gccgcaggta tttcagcatt
     8761 acttggtatt acagaaccgg ctatgtttgg tgttaactta aaactaagat atccatttat
     8821 tggcgctatc gttggatcag gtattggttc agcatatatt gctttcttca aggttaaagc
     8881 aatcgcatta ggaactgctg gattgccagg atttatttca atcaatccag tacatgcagg
     8941 atggttacac tactttgttg gtatgacaat atcattcatc attgctataa cagttacttt
     9001 aattttatct aaaagaaaag caaataaaga agttgtagaa taaaaaaaga ggtatgacag
     9061 taatcaaagt atcagtcatt gcaaatgcaa taggatgaaa ctttaagctg tcataccttt
     9121 taatttagtt ataatttctg tttacgccat aagaaaatac taacaattac gacaagaatt
     9181 aatgaaatac ctaccacaat aatccaagat atataactat ggtcatcaat tggcaacggc
     9241 acattcatac cgaagaaact aaatacgagt gttggtaacg ttaatagtac cgtgaaaagt
     9301 gttaatgtct tcataatcgt attcatatca ttagataata aggaagcgta tgatgttgtg
     9361 atactttcta agatacgttg atgtaattct gttgtttcga tggcttggtt attttcaatg
     9421 attaaatctt ctagtaattc ttcatcttct tcaaaacgtt taattgcagg taaacggaat
     9481 aactttttaa taattgtatc gttacctttt aaggcagcta aaaagtatac taaacttttt
     9541 tcaacttcca ttaatttgaa aagttgtttg ttagtaatgt tattttttag ttctttctca
     9601 atacgaattc tacttttatt aagtaatcgt aagttacgat tataatggtc ggcaatagta
     9661 gttaaaattt ctaatgcaaa tctgctgtga aattttaaat taatatcacg cttaggtaaa
     9721 ttttctaaaa attcattttc agcgtcacaa actgttacga taataccgtt gccaataata
     9781 atgcctaatg gaatcgttac aaaagataga acgctacgat tagttgaatt gacgataggt
     9841 aaatcaataa tgattaatga gtaaccagta tcttcgtcat attcaatacg ggaactttct
     9901 tctgagtcca aagggtcacg tataaagtct tcaggtatat tatattgttc cataagattt
     9961 tcaatttctt ctcggtctgg ttctactaca ttaatccatg acgctgtatg atctaatggt
    10021 gtttttataa tatgtttatc tatatcttca gaatgtctga atgaagtaat catgatatat
    10081 agttcctttc taaaggattt aggaaaagcg agtattactt aagttagtga atcaaggttt
    10141 gatttatgca aacggttttc tgccattatt cttaatggac cattaaaaat aatattatgc
    10201 acatgtttag ctaattcatc aactgaaatg ggctgtttgt ccattaccca gtatttaata
    10261 attgaaattg ttgctccgga aacgtaactg taaaaataca tctctggaac gcctccgata
    10321 gaatgattga tactaatgta acgttgcata ttatttttaa tatgttcgtt gattttcaat
    10381 tctaattgac tcgtgcgttc caattgtaaa atagtatgat aaaactctaa attatcatga
    10441 atatgttgga aaatattttt gagtatatta tttacaaaag tattcgcaat gtcttcaatt
    10501 gaagaaccgc gtaacaaatc aaattgagta tatgtcgtta gttctgaaat atactcatct
    10561 tccatatctg cgagtaacat atatttatcc tcgtaatgta agtaaaatgt ccctctattg
    10621 atatcagcgc gtgttgttat gtcgcgaatg gtaatctttt ctaaatcttt ttcttgtagt
    10681 aatttagtga acgcttgttt aatggaggac tttgtctttc taattcgcct atcttctttc
    10741 aaaaataaat caacctcctt gtgttattga acaatgtggg tgtatttgtc tattaatgaa
    10801 cgtattgatg aacattgtgt attgtttatt cccacttaca tattataata gacaacatgc
    10861 gaaatattca acacattgtc taataaggag gtaattaata aaatgaatat atttaaaaat
    10921 aaattattat ggattgcacc aatagcaact atgattatct tggtaatctt ttctttagct
    10981 ttttatcctg catataatcc taaaccaaaa gatttaccaa ttggtatatt aaacgaggat
    11041 aaaggtacaa cgattcaaga taaaaatgtt aacattggta aaaaattaga ggataaatta
    11101 ttagatagtg attctaataa aattaaatgg gttaaggttg atagtgaaaa agaccttgaa
    11161 aaagatttga aagatcaaaa aatctttgga gtagctatta ttgataaaga cttttcaaaa
    11221 gatgctatga gtaaaacaca aaaagtagtt atggatagta aaaaagaaga aatgcaacaa
    11281 aaagttgctt caggtgaaat tccgccacaa gtggttcaac aaatgaaaca aaaaatgggg
    11341 aatcaacaag tagaggttaa gcaggctaaa tttaaaacga ttgtaagtga aggatcaagc
    11401 ttacaaggtt cacaaattgc atcagctgtg ttaactggta tgggtgataa tattaatgct
    11461 caaattacga agcaaagttt ggaaacatta acgagtcaaa atgttaaagt caatgccgcg
    11521 gacatcaatg gtttgacgaa tccagtaaaa gtggataatg aaaaacttaa taaagttaaa
    11581 gatcaccaag caggtggtaa tgcaccattc ctaatgttta tgccaatttg gataggttca
    11641 atcgtaacgt ctatcttatt gttctttgca tttagaacta gtaacaatat cgtcgtgcaa
    11701 catcgtatca ttgcttcaat tggacagatg atatttgcag ttgttgcagc atttgcaggt
    11761 agctttgttt atatttattt catgcaaggc gttcaaagat ttgattttga ccatccaaat
    11821 cgtatcgcaa tttttgtagc atttgcgatt cttggtttcg tgggccttat tttaggtgtt
    11881 atggtatggc taggtatgaa gtcagttcca attttcttca ttttaatgtt ctttagtatg
    11941 caacttgtaa cgttacctaa acaaatgttg cctgaaagtt atcaaaaata tgtatatgat
    12001 tggaatccat tcacacacta tgcaacaagt gtaagagaac tattatactt gaatcatcat
    12061 attgaattaa atagtacaat gtggatgttt atagggttta tgatttttgg cgctgtatca
    12121 agtttagttt cagctattgt tagaaaacat agcacaaaac gtactgaagt gccgtcataa
    12181 tttaataaat agaaataata gctatacaag acgtttgtag cgagcgtcat ggtatagcta
    12241 ttttatttgt atagcgcttg atgtaaagtt attttagctg tcatactatg tgctaaacca
    12301 aattcagttg tgtcatatct tcgctttttt gtcactgtta taataggtat aatcggatat
    12361 aatgaaaagg tgagggagga ttcaaaatgc gtaggttatt atattcattt cttttttata
    12421 tgatcatagg tttatttagt ggctttttct atagagagct aacaaaagca tatgatttta
    12481 ctggtacaac acaattatca cttgtacaca cacatacact tattttaggt atgtttatgt
    12541 ttttaatatt attaccattg gagaagttat ttaaattatc aagttactac ttatttaact
    12601 ggttcttcta tgtatataac ataggtgtta tcgttactat aggtatgatg gtgacaaaag
    12661 gattcttcca agtcactggt aaatcatact ctccagaagc atttgcaggc tttgcaggta
    12721 ttgggcacac aggtatgctt gcaggtttat tgttgctatt tttcctttta agacaagcaa
    12781 ttcttaaaga accacgtgat gagtctttta aaaaataata taactagaac taaaaaatat
    12841 acacatcttt caaaggaaca ttgttgtttc ttactttgaa aggtgtgttt ttatattgtg
    12901 atgggatagt tattgataat tcatggctaa ctatgcgatt tattttaaca atataaatat
    12961 taatagtata atggctttaa atgataataa ttatcaattt gagcacgtta atgaaagaaa
    13021 tgaatataga gagtcatagg aggcacataa tgaaagatgt tacaatcatt ggtggcggtc
    13081 cgtctggttt gtatgcaagc ttttatgctg gattgcgaga tatgtctgta agattgatag
    13141 atgttcaatc tgaattaggg ggtaagatga gaatttaccc tgaaaaaatt atttgggata
    13201 ttggcggcat tgcgccaaaa ccttgtcatg aaattttaaa agatacaatt aagcaaggat
    13261 tatattttaa accggaagtt catttgaatg aacgtgtggt agatattagg aaaaaggcag
    13321 aacgtcattt cgaagttgaa acagaagcgg gagaaatata tacatcgaaa gcagttatca
    13381 ttgcaattgg tgcgggtatt attaatccaa aacaattaga tgttaaaggt gtagagagat
    13441 atcaattaac taatttacat tatgttgtac aaagttacag acgtttcaaa gataaagatg
    13501 tattaatatc aggtggagga aatacagcat tagattgggc gcatgatatt gccaagattg
    13561 ctaaaagcgt gacagttgtt tatcgaaaag aagatgtaag tggtcatgaa gcgatgaaaa
    13621 cgttggtgac agatttaaat gtgaaactat gcccaaaaac acgtattaaa tacttggtcg
    13681 gcaatgatga tgaaacgcat ataagtgaag ttgttttgga acatgttgaa agtggcgata
    13741 ggcatacagt taaatttgac gatgtcatta ttagtcatgg gtttgatcgt tgtaatacat
    13801 tattgagtga aacatcttca aagctagata tgcatgatga ttgtcgtgtt aaaggatttg
    13861 gtaatacgac aactagtata cctggtatct atgcgtgtgg agatattgtt tatcatgatg
    13921 caaaatcaca tctaattgca agtgcattta gtgatggtgc aaatgcagcg aaccttgcta
    13981 aaacatatat ccaaccagat gcgaatgcag agggttatgt ttcaagtcat catgaagtgt
    14041 ttaaagaagc gaataagact attgtaaata aacatttata ctaggttgat atattgtgtg
    14101 atgaaaagtg gggtaaataa tggttaaagt gacatatgat attccgactt gcgaggatta
    14161 ttgcgcatta aggattaacg caggtatgag tccaaagacg cgcgaagcag ctgaaaaagg
    14221 attacctaat gccttattta cagtaacctt gtatgataaa gatcggttaa ttggtatggg
    14281 tagagtgatt ggcgatggcg gaactgtttt tcaaattgtt gatattgcag ttttgaaaag
    14341 ttaccaaggt caaggttacg gcagtctaat tatggagcat attatgcaat atattaaagg
    14401 tgtggctgtt gagagtacat acgttagtct gattgcagac tacccagcgg ataaattata
    14461 tacaaaattt ggatttatac ctaccgaacc agattcaggc ggtatgtata tcaaatacta
    14521 aattaaaaaa gacatctcgc ataacgggat gtctttttca tgaatactat ttatctacac
    14581 cgaaatcgat aacaatgcgc cctttatttt catgtttaat tactttgtta agttgttctg
    14641 gaagttcatc aaatgtaata acttgcttga tctcatgtaa attttcaggc attaaatctt
    14701 ttgcgagacg acgccaaacg cgctgtctta atttcatagc agtaaatacc gagtcaattc
    14761 ctaaaatgtt tacacctctt aaaatgtgag ggaatacaga attagtataa gtattaccgg
    14821 cagtcatacc aataactgca attgacccac tatgatttaa acgctttgta acataattaa
    14881 taccttcgcc accaacaggg tctacacaag cttgccaagt tgatgatgcg agtggctttt
    14941 tatgatcatc ttcaacagga agtcgatcga taacttcttt ggcaccaagt tcaagtaatt
    15001 gatcgctaac atcttgttta cctgtacttg cgataacttt ataaccaagt tcattaagca
    15061 tgagtactgc taaagtaccg acaccacctg aagcaccgcg aacgagtaca ggaccatctt
    15121 caatattcat tccaactttt tcaagtcttt caattgctaa accggcagta taaccagctg
    15181 tgccatatat cattgattct tctaatgtta aagtatcagg aagcttgata atccattctg
    15241 attttacacg cgcatattca ctaaaaccgc cataatggct gacacctagg tcataactcg
    15301 ttacaattac ttgttctcct ttttcaaagc ctggtgcttc ggattcaaca attgttccag
    15361 ctaaatctat tcctggaatc ataggatacg attttacgac tgcattatga tcttgagtcg
    15421 ctaaagcatc tttataattt ataccagagt aatgtacttt aatcagcaca tctcctttag
    15481 gtaaatcagt aggcgataat tgtttgaaag ttggtgtcac tttaccactc tcatctttat
    15541 caattacaaa cgctttaaat gtttctatca caacgataca tcctctcttt ttctcttttg
    15601 aaaatacggc cattttactg gaataaatgt atcatttatt gatgttacac acaatcaata
    15661 caatttgtaa ttgatttgtg ttaatgtatt tttaaatagt tttggctgaa aaataacata
    15721 agtaatattt tgattcgatc actgtttatt ggaggattta tatggctggg ataatcaaag
    15781 aaatttcaga acaaaatgct agtgaattag ttgaattagc aactagaaca ttttatgaca
    15841 cgtttggttc ttactatgat gacaaagatt ttgatcaatt ttttaaagac aattatactg
    15901 tagagaaatt tacacaagag attaaccatg tagattcatt tcattatttt tatcaagaag
    15961 atggtgcgaa tgttggttat ataaagatga atattaatag tgctcaaact gaagaaatgg
    16021 gggagaccta tttagaagtg cagcgcatat attttttgaa agactttcaa ggtggcggaa
    16081 gaggttcaca attgatagaa ttggccgaaa aaattgctca agaacataat aaacataaaa
    16141 tttggctagg agtttgggag cataatcctc gtgctcaagc attttataaa cgtcatggat
    16201 ttaaagtggt tggtgaacat cattttcaaa caggtgatgt aacggataca gatttaatta
    16261 tggaaaaaga gttataatga ttgtttttgc agcagataaa atgaaatcat agtaataaat
    16321 attgtgtata ccttgaaatg ctaaatgcgt aatgaattgt taaagtaaaa taattaaatt
    16381 tatagaaaac ataaaattca caaaaattaa ataataatga acaaaaatta gggtataata
    16441 aaaagaacct tagattgtag ttatggatat tgtgataatt cagatcatat taatgttgac
    16501 gtctttggta acaagccatg aaaagataca catggtagat atgtatttca agattattca
    16561 atgaatatcg aattatagga ggagatatgt atgaaaagat tagttacagg gttactagca
    16621 ttatcattat ttttagctgc atgtggtcaa gatagtgacc aacaaaaaga cggtaataaa
    16681 gaaaaagatg ataaagcgaa aactgaacaa caagataaaa aaacaaatga ttcatctaaa
    16741 gataagaaag ataataaaga tgatagtaaa gacgtaaaca aagataataa agataatagt
    16801 gcaaacgata accagcaaca atctaattca aatgcaacaa acaatgacca aaaccaaaca
    16861 aataataacc aatcaagtaa taaccaagcg aataataatc aaaaatcaag ttacgttgca
    16921 ccatattatg gacaaaatgc cgcgccggtt gcacgtcaaa tttatccgtt taatggaaat
    16981 aaaaatcaag ctttacagca attgccaaat ttccaaacag ctttaaatgc ggctaataat
    17041 gaagcaaata aatttggtag taataataaa gtgtataatg attattctat tgaagaacat
    17101 aatggcaact ataagtatgt gtttagtttt aaagacccaa atgcaaatgg aaaatattca
    17161 attgtaacgg ttgattatac tggacaagca atggttactg atccaaacta ccaacaataa
    17221 tgttaatata actatgatgc aagttaaaaa ataaaacggt aaactctata atatgaatta
    17281 gggtttaccg ttttttgcgt attttaaagt atcaaagttt acgttttata gtacttttaa
    17341 acctcatt
//
LOCUS       DAKTIO010000045        17141 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.045, whole genome shotgun sequence.
ACCESSION   DAKTIO010000045 DAKTIO010000000
VERSION     DAKTIO010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17141
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.045"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..104
                     /locus_tag="O0293_001109"
     CDS             <1..104
                     /locus_tag="O0293_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280001.1"
                     /translation="VCTPTIERIYLTNSKNIVVFLEKSKHDNKNVKV"
     gene            415..1368
                     /locus_tag="O0293_001110"
     CDS             415..1368
                     /locus_tag="O0293_001110"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCY0280002.1"
                     /translation="MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKV
                     RGDVMDLKHATPYSPTTVRVKAGEYSDCHDADLVVICAGAAQKPGETRLDLVSKNLKI
                     FKSIVGEVMASKFDGIFLVATNPVDILAYATWKFSGLPKERVIGSGTILDSARFRLLL
                     SEAFDVAPRSVDAQIIGEHGDTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQT
                     RDAAYDIIQAKGATYYGVAMGLARITEAIFRNEDAVLTVSALLEGEYEEEDVYIGVPA
                     VINRNGIRNVVEIPLNDEEQSKFAHSAKTLKDIMAEAEELK"
     gene            complement(1688..3217)
                     /locus_tag="O0293_001111"
     CDS             complement(1688..3217)
                     /locus_tag="O0293_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCY0280003.1"
                     /translation="MKSLFEKAQQFGKSFMLPIAILPAAGLLLGIGGALSNPNTVKAY
                     PILDITLLQNIFTLMSAAGSIVFQNLPVIFAIGVAIGLSRSDKGTAGLAALLGFLIMN
                     ATMNGLLTITGTLAKDQLAQNGQGMVLGIQTVETGVFGGIITGIMTAILHNKYHKVVL
                     PPYLGFFGGSRFVPIVTAFAAIFLGVLMFFIWPSIQAGIYHVGGFVTKTGAIGTFVYG
                     FILRLLGPLGLHHIFYLPFWQTALGGTLEVKGHLVQGTQNIFFAQLGDPDVTKYYSGV
                     SRFMSGRFITMMFGLCGAALAIYHTAKPEHKKVVGGLMLSAALTSFLTGITEPLEFSF
                     LFVAPILYVIHAFFDGLAFMMADIFNITIGQTFSGGFIDFLLFGVLQGNSKTNYLYVI
                     PIGIVWFCLYYIVFRFLITKFNFKTPGREDKAAAQQVEATERAQTIVAGLGGKDNIEI
                     VDCCATRLRVTLHQNDKVDKVLLESTGAKGVIQQGTGVQVIYGPHVTVIKNEIEELLG
                     D"
     gene            3579..4514
                     /locus_tag="O0293_001112"
     CDS             3579..4514
                     /locus_tag="O0293_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCY0280004.1"
                     /translation="MKRKIIMDCDPGHDDAIALILAGAIDSPLEILAVTTVAGNQSVD
                     KNTTNALNVLDIMGRQDIAVAKGADRPLIKPAAFASEIHGESGLDGPKLPSTPSRQAV
                     AMPASDVIINKVMTSDTPVTIVATGPLTNVATALIREPRIAEHIESITLMGGGTFGNW
                     TPTAEFNIWVDAEAAKRVFESGITINVFGLDVTHQVLADDHVIERFESINNPVAQFVV
                     ELLQFFKKTYKTHFNMDGGPIHDACTILYLLQPELFTMVPVNIDIEHQSPLTYGTMAV
                     DLNHVTGKPANAYFATAVDVEEVWNLIDHKLRTYE"
     gene            4624..4722
                     /locus_tag="O0293_001113"
     CDS             4624..4722
                     /locus_tag="O0293_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280005.1"
                     /translation="MYVKNASKKHKTQKYMQKFQLLYFKRLKSQKI"
     gene            4853..6949
                     /locus_tag="O0293_001114"
     CDS             4853..6949
                     /locus_tag="O0293_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCY0280006.1"
                     /translation="MNGDNQQILREIVLNPTIHGKELESIFGLSRRQLGYRIQKINLW
                     LEQEGYPKLERTSQGNFIVSSEIMTLFKRDVSEQQMLNGNNVIFSIETRRYYLMLMLF
                     SKENAMSLNHFSIDLQVSKNTVIHDINHVKEQLENHGLSLKYSRKHGYEIVGDEFEVR
                     RFFIKLIDQRLNHDITKSEVLKALNLTFEDIAYQKDKIKQVEQFLKSRFIDKSLSSLP
                     YVLCVIRRRIQSGHVMNPLNINYQYLRDTKEYQATEIMTQHEPDLPEAEKLYLTLHLL
                     STSVQWTDLQESDSISNLTMAIAQMIHHFEQITFINIEDKEKLSQQLLLHLTPAFYRI
                     KYNLTDRDELINPLQGNYQSLFHMVKQSCQSLTEYFGKSLPDNEIAYLTMLFGGSLRR
                     QDENFDGKIKAIIVCTQGTSVSQMMLYELRNLFPEIIFLDAISLRTFENYTLDYDIVF
                     SPMFVLTHKKLFITKVALSENEQRKLRKEVMKYINKESADIDKEINKLMALIERTTTV
                     NDITELRDGLEDFIANYNSISTINGSIVTQNKTLDLADLIPARHVKRMHHVENIDEAI
                     AKASDVLVANHFIDIKYIHEMQQVFDDSYMVIMQNIAIPHAYSEKHVHKTAMSMLILQ
                     EPIYMSDGTAIHIIVPIAAVDKVTHLRALLQLRDVAQDNDAIKRIIQSRKNSDVNEIL
                     KNYSNKEARENGWDSN"
     gene            6934..7401
                     /locus_tag="O0293_001115"
     CDS             6934..7401
                     /locus_tag="O0293_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCY0280007.1"
                     /translation="MGQQLVHKENIMLNLSATDKESVLSQMSDVLFQNGFVKSTFKDA
                     VIDREKEFATGLPTHLCSVAIPHTDVEHINHRTIGVAVLEKEVPFIEMGTLDQQTEVK
                     IVFMLAMDKVDDQLKLLQQLMQIFQSEEKLEQILRTKDETILATLINDYLEYN"
     gene            7424..7702
                     /locus_tag="O0293_001116"
     CDS             7424..7702
                     /locus_tag="O0293_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCY0280008.1"
                     /translation="MKQVLVACGAGIATSTVVNNAIEEMAKEHNIKVDIKQIKITEVG
                     PYEDTADLLVTTAMTKKEYKFPVINARNFLTGIGIEETKQQILTELQK"
     gene            7929..9188
                     /locus_tag="O0293_001117"
     CDS             7929..9188
                     /locus_tag="O0293_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCY0280009.1"
                     /translation="MSYFTDFVRGFLDLGATVILPVVIFLLGLFFRQKIGAAFRSGLT
                     IGVAFVGIFLVIDLLVKNLGPAAQAMVKNLGVSLNVIDVGWPATSSIAWASSVAAFII
                     PLGIIVNVVLLVTKVTKTMNVDIWNFWHYTFTAAMVYAVSGSIWQALLAAVIFQIICL
                     KVADWTAPMMSEFFDLPGVSIATGSTISYAPGIYLVKLLQKVPGLNKLDADPETIQKR
                     FGAFGESIFVGLILGLGIGVLAGYKPGDIINLGMSMAAVMVLMPRMVKILMEGLMPVS
                     ESARTWLNKRFGEREIYIGLDAAVALGHPAVISTALILVPITVLLAVILPGNQVLPFG
                     DLATIPFVVAFIVGAARGNIIHSVIVGTIMIAISLYIATDVAPIFTDMAKGTNVQMPK
                     GSSEISSIDQGGNIVNYLIFKLFSLFN"
     gene            9206..10261
                     /locus_tag="O0293_001118"
     CDS             9206..10261
                     /locus_tag="O0293_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCY0280010.1"
                     /translation="MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIH
                     TYEGHYKVNFPVTLGHEFSGEIVEVGADVKDFKVGDRVTSETTFYVCNECEYCKSKDY
                     NLCNHRKGIGTQVDGAFTNYVIAREESLHHIPDEVSYQSAAMTEPLACAYHGVSKIQV
                     NSGDVAVVMGPGPIGLLVAQVLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQT
                     DLKTYINGITDGYGADVVVECSGAVPAARQGLDILRKKGFYSQIGIFKDAEIPFDMEK
                     VIQKEITVVGSRSQKPADWEPSLQLMADGLVNAEALVTKIYDISKWDEAYQHLKSGEG
                     IKVLLKPLDLDENEGEN"
     gene            10263..10409
                     /locus_tag="O0293_001119"
     CDS             10263..10409
                     /locus_tag="O0293_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280011.1"
                     /translation="MVESMLTFMLGPLRQITDFYMEHLLVSNSIVIAGYFATGIFKKK
                     KVVN"
     gene            10433..11476
                     /locus_tag="O0293_001120"
     CDS             10433..11476
                     /locus_tag="O0293_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCY0280012.1"
                     /translation="MKALKLYGVEDLRYEDNEKPVIESANDVIIKVRATGICGSDTSR
                     YKKMGPYIKGMPFGHEFSGVVDAIGSDVTHVNVGDKVTGCPAIPCYQCEYCLKGEYAR
                     CEKLFVIGSYEPGSFAEYVKLPAQNVLKVPDNVDYIEAAMVEPSAVVAHGFYKSNIQP
                     GMTVAVMGCGSIGLLAIQWARIFGAAHIIAIDIDAHKLDIATSLGAHQTINSKEENLE
                     KFIENHYANQIDLAIESSGAKVTIGQILTLPKKGGEVVLLGIPYDDIEIDRVHFEKIL
                     RNELTVCGSWNCLSSNFPGKEWTATLHYMKTKDINVKPIISHFLPLEKGPETFDKLVN
                     KKERFDKVMFTIY"
     gene            12004..12720
                     /locus_tag="O0293_001121"
     CDS             12004..12720
                     /locus_tag="O0293_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCY0280013.1"
                     /translation="MIYAGILAGGIGSRMGNVPLPKQFLDIDNKPILIHTIEKFILVS
                     EFNEIIIATPAQWISHTQDILKKYNITDQRVKVVAGGTDRNETIMNIIDHIRNVNGIN
                     NDDVIVTHDAVRPFLTQRIIKENIEVAAKYGAVDTVIEAIDTIVMSKDKQNIHSIPVR
                     NEMYQGQTPQSFNIKLLQDSYRALSSEQKEILSDACKIIVESGHAVKLVRGELYNIKV
                     TTPYDLKVANAIIQGDIADD"
     gene            12713..13738
                     /locus_tag="O0293_001122"
     CDS             12713..13738
                     /locus_tag="O0293_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCY0280014.1"
                     /translation="MINQVYQLVAPRQFDVTYNNVDIYGNHVIVRPLYLSICAADQRY
                     YTGRRDENVLRKKLPMSLVHEAVGEVVFDSKGVFEKGTKVVMVPNTPTEQHHIIAENY
                     LASSYFRSSGYDGFMQDYVVMAHDRIVPLPNDIDLSTISYTELVSVSYHAIQRFERKS
                     IPLKTSFGIWGDGNLGYITAILLRKLYPEAKTYVFGKTDYKLSHFSFVDDIFTVNQIP
                     DDLKIDHAFECVGGKGSQVALQQIVEHISPEGSIALLGVSELPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFEQVVDLYRKYPDIVEKLALLKGHEINVCTMQDIVQAFEMDLSTSWGK
                     TVLKWTI"
     gene            13760..15454
                     /locus_tag="O0293_001123"
     CDS             13760..15454
                     /locus_tag="O0293_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCY0280015.1"
                     /translation="MTKTKQAIHIDNIYWERVQLYIEGHSEGVDLTSGQFVLRNLTET
                     KTLEANEMKIDGNTFICRFNVAILDDGYYLPMDKYLFVYHDQLEYIGQLNPNIIDQAY
                     AALNEEQIEEYNELTTQNGKVNYLLAYDAKVFRKGGVSQHTVYTITPEIASDVNEFVF
                     DIEITLPQEKSGVIATSAHWLHKQGHKASFESRSFLFKAIFNITKLLHIKRSKTILFT
                     SDSRPNLSGNFKYVYDELLRQKVDFDYDIKTVFKENITDRRKWRDKFRLPYLLGKADY
                     IFVDDFHPLIYTVRFRPSQEIIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKA
                     YVSSETDIPFYAEAFGIREENVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKV
                     ILFAPTFRGNGHGTAHYPFFKIDFERLARYCEKHNAVVLFKMHPFVKNRLNISREHRQ
                     YFIDVSDHREVNDILFVTDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEP
                     FESFVPGKIVQSFDALMDALDNEDYEVEKVVPFLDKHFKYQDGRSSERLVKDLFRR"
     gene            15882..17051
                     /locus_tag="O0293_001124"
     CDS             15882..17051
                     /locus_tag="O0293_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol--glycerophosphate
                     glycerophosphotransferase"
                     /protein_id="HCY0280016.1"
                     /translation="MIKNTIKKLIEHSIYTTFKLLSKLPNKNLIYFESFHGKQYSDNP
                     KALYEYLTEHSDAQLIWGVKKGYEHIFQQHNVPYVTKFSMKWFLAMPRAKAWMINTRT
                     PDWLYKSPRTTYLQTWHGTPLKKIGLDISNVKMLGTNTQNYQDGFKKESQRWDYLVSP
                     NPYSTSIFQNAFHVSRDKILETGYPRNDKLSHKRNDTEYINGIKTRLNIPLDKKVIMY
                     APTWRDDEAIREGSYQFNVNFDIEALRQALDDDYVILLRMHYLVVTRIDEHDDFVKDV
                     SDYEDISDLYLISDALVTDYSSVMFDFGVLKRPQIFYAYDLDKYGDELRGFYMDYKKE
                     LPGPIVENQTALIDALKQIDETANEYIEARTVFYQKFCSLEDGQASQRICQTIFK"
BASE COUNT         6004 a         2569 c         3352 g         5216 t
ORIGIN      
        1 ctgtttgcac accgacgatt gagcgcattt atttgactaa ttcaaaaaac attgttgttt
       61 tcctagaaaa aagtaaacat gataataaaa atgtgaaagt gtaaataatc actggcgaag
      121 tacgaagact aaagacatct aagatgtaat cgtatacaaa ttaaaaaggt gtaaaaatta
      181 aaataaaatg tgaaataaat cacaatttaa tattgaccca gtacttaatg catgttacat
      241 tttatatgtg aaataaatca caaacttaaa agcggatgac acatgacctt ttaagttatg
      301 cgttgaaaat aaaagagatg tttatttgct tttgtatcgt caataagcag cattaaacta
      361 acatatttga agctacatgt atgttagtga attaatcata agggagtttt tgtaatgaac
      421 aaatttaaag ggaacaaagt tgtattaata ggtaatggtg cagtaggttc aagctacgca
      481 ttttcattag tgaaccaaag cattgttgat gaattagtca tcattgattt agacactgaa
      541 aaagttcgag gagatgttat ggatttaaaa catgccacac catattctcc aacaacagtt
      601 cgtgtgaaag ctggcgaata cagtgattgt catgatgcgg atctagttgt catctgtgct
      661 ggtgctgcac aaaaacctgg agaaacacgt ttagatttag tatctaaaaa cttgaaaata
      721 ttcaaatcaa ttgttggtga agtaatggca tcaaaatttg atggtatttt cttggtagct
      781 acaaatcctg ttgatatttt agcgtatgca acatggaaat tctctggttt acctaaagaa
      841 cgtgttatag gttctggtac aattttagac tctgcacgct ttagattatt gttaagcgaa
      901 gcgttcgatg ttgcgccacg tagcgtcgat gctcaaatta ttggtgaaca tggtgacact
      961 gaattaccag tatggtcaca cgctaatatt gcgggtcaac ctttgaagac attacttgaa
     1021 caacgtcctg agggcaaagc gcaaattgaa caaatttttg ttcaaacacg tgatgcagca
     1081 tatgacatta ttcaagctaa aggtgccact tattatggtg ttgcaatggg attagctaga
     1141 attactgaag cgattttcag aaatgaagat gccgtattga ctgtatcagc attattagaa
     1201 ggcgaatatg aggaagaaga tgtttatatt ggtgttccag cagtcatcaa tagaaacggt
     1261 attcgcaacg tcgtagaaat cccattaaac gacgaagaac aaagcaagtt cgcacattca
     1321 gctaaaacat taaaagatat tatggctgaa gcagaagaac ttaaataact ttttataaaa
     1381 tctataccat cccaaaaatt gtaaaacctt acccaaaaaa ttgtataaag ggctatttga
     1441 tacaactata tgtgtcgagt agccctattt ttaatgtagt gaaagtcgtt gttgaaatta
     1501 aattcaaatc tggcacttaa gctttaatca tagcattaag atgattgtct agcagaggcg
     1561 atttgcgggc tcactacagt gcatgatgaa cttaatgctt caaatgtaac attaaaaata
     1621 aaagcaacga tgtcacttct tacttcgtac atcgttgcca ttagttctgt tgatatttcg
     1681 gttagtctta atccccgagc aattcttcaa tttcattttt gataactgta acgtgaggcc
     1741 cataaattac ttgcacacca gtgccttgct ggattacacc tttggcacca gtactttcga
     1801 gtaatacttt atcgactttg tcattttgat gaagtgtgac gcgtagtctc gttgcacaac
     1861 agtcaacgat ttcaatgtta tctttgcctc ccaaaccagc aacaatagtt tgtgctcttt
     1921 cagtagcctc aacttgttgt gctgcagctt tatcttctcg accaggtgtt ttgaaattaa
     1981 atttcgtaat taagaatctg aaaacgatgt aatacaaaca gaaccacaca attccaatag
     2041 gtatgacgta taggtagttt gttttactat taccttgtag cacaccaaag agtaagaaat
     2101 cgataaagcc tccactgaag gtttgaccaa ttgtaatgtt gaaaatgtct gccatcataa
     2161 atgctaatcc atcaaagaag gcatggatta cataaagaat aggtgcgaca aacaagaaac
     2221 taaactctaa aggttcggta atacctgtta aaaatgaagt gagtgcagcg gataacatta
     2281 aaccgccgac aactttttta tgttcaggtt tagctgtgtg ataaattgca agtgcggcac
     2341 cacataagcc gaacatcatc gtaataaaac ggcctgacat aaagcgtgac acacctgaat
     2401 aatacttcgt cacatctgga tcaccaagtt gagcaaagaa gatgttctgc gtaccttgaa
     2461 ctaagtgccc tttgacttct aaagtaccac caagtgccgt ctgccaaaac ggtaagtaaa
     2521 aaatatggtg taaaccgagt ggacctaaca atcttaagat gaagccataa acaaaagtac
     2581 cgatggcacc tgttttcgtt acaaatccac caacatgata aatgccggct tgtatgcttg
     2641 gccaaatgaa aaacatcaat acacctaaaa agattgcggc aaatgctgtg acaataggga
     2701 caaatctaga gccaccaaag aaacctaaat acggtggtaa taccactttg tgatatttgt
     2761 tgtgaagtat tgcggtcata atacctgtga taatcccgcc aaaaacaccg gtttcaaccg
     2821 tttgtatacc gagcaccatg ccttgtccat tttgtgcaag ctgatctttt gccaatgtgc
     2881 ccgtgatagt taataagcca ttcatagttg cgttcataat taagaaaccg agcagcgcag
     2941 ctaaacctgc agtaccttta tcgcttctag ataatccgat tgcgacacca attgcaaaga
     3001 tgaccggtaa attttggaaa acaatactac ctgcagctga cattaatgta aaaatatttt
     3061 gtaataaggt aatatctaaa atagggtatg ctttaacggt gtttggatta cttaatgcac
     3121 caccgatacc caacaataga cctgcagctg gtaagattgc gataggtaac ataaaggact
     3181 tgccgaactg ctgtgctttt tcaaataaag atttcatcaa catccctcct aattattctc
     3241 aatatagctt ttgagaaatt taatatcaat atatattctg tgtatgaaaa tatttttcat
     3301 aaaaattgtt tgaatcatgt aacaatcata taaattgctg tttatattgt tgtgaaaatg
     3361 agttgacaaa agtcggtata gatatgtaga catcctattt ttagcgaggt aggttgataa
     3421 ggcatatcgg ataaatttat aagccataaa atagataaat agtgtttgta tccaaaaata
     3481 tgagaaagtt aaaactattt ttcaaaataa acatgattca ccacataaat aaatatacat
     3541 aagctatcga taacaactta agaaaggtgg atatataaat gaaaagaaag attattatgg
     3601 attgtgatcc aggacacgat gatgcaatag cattaatttt agcgggggca attgacagtc
     3661 cactagagat attagctgta acaacagtcg caggtaatca atcagttgac aagaatacga
     3721 caaacgcctt gaacgtattg gatattatgg gacgccaaga tatagcagta gcgaaaggtg
     3781 cggataggcc gttaattaaa ccagctgcct ttgcttctga aatacatggg gaatctggat
     3841 tagatggtcc gaaactaccg tcgacaccat cacgtcaagc agttgcaatg ccagcatcag
     3901 atgtgattat aaacaaagtg atgacgagtg atacacctgt aacaattgta gcgacaggtc
     3961 ctcttacgaa tgtagcaacg gcattgattc gtgagccaag aatcgctgag catattgaat
     4021 ctattacttt gatgggtggt ggtacatttg gaaattggac gcctacagca gaattcaata
     4081 tttgggtaga tgctgaagca gcgaagcgtg tttttgaaag tgggattact ataaatgtgt
     4141 ttggtttaga tgtaacacat caagttttag ccgacgatca cgtgattgaa cgctttgaaa
     4201 gtatcaataa tcctgttgca cagttcgtcg tagaattatt gcaattcttt aagaagacat
     4261 acaagactca ctttaatatg gatggtggtc caatacatga tgcttgtaca attttgtatt
     4321 tgttacaacc agaattgttt acaatggtac ccgttaatat cgacattgaa catcaaagtc
     4381 cactaactta tggcactatg gctgtcgatt taaatcatgt tacaggtaag cctgccaatg
     4441 cttattttgc tacagcagtt gatgttgaag aagtgtggaa cttgatagac cataagttac
     4501 gtacatacga ataataatac ttaattaaat agatacagtt aaccctaagg cgcctgatat
     4561 aagcgtcctt agggtttttg tattgggtta caatggtcat gacaatttga taatgattga
     4621 gatatgtatg ttaaaaatgc ttcaaaaaaa cacaaaacac aaaaatatat gcaaaaattt
     4681 caattgcttt attttaaaag attaaaatca caaaaaatat agatataatt gaaatgttgt
     4741 taaaacatca caatgatgta catgctcatt ttgaaaatat gtgttcaaag tgtgtacatc
     4801 ggtgtgacga ttgatgataa attgagctca gatgctcaaa ggagaatcga acatgaacgg
     4861 ggataatcag caaatactca gagaaattgt attgaatcct actattcatg gtaaagaact
     4921 tgaatcgata tttggtttgt ctcgtagaca actaggatat cgcattcaaa aaatcaattt
     4981 gtggcttgaa caagagggtt atccaaaact tgaaagaaca agccaaggaa attttattgt
     5041 aagttctgaa atcatgacgt tattcaagcg agatgtatcg gagcagcaaa tgttaaacgg
     5101 caacaatgtc atttttagca tagaaacacg tcgttattat ttaatgctca tgctttttag
     5161 taaggaaaac gcaatgtctc taaaccattt ttcaattgat ttacaagtca gtaaaaatac
     5221 tgtcattcac gatataaatc atgtgaaaga gcaattggaa aatcatggtt tgtcacttaa
     5281 gtattctcga aaacatggtt atgaaattgt tggtgatgaa tttgaagttc gccgtttctt
     5341 cattaagttg attgatcaaa ggttgaatca tgatattact aaaagtgaag ttttaaaggc
     5401 gctcaactta acattcgaag atatcgcata tcaaaaagac aagatcaaac aggtagaaca
     5461 atttttgaag agtcgcttta tagacaaatc acttagttca ttgccttatg tcctttgtgt
     5521 gattcgtaga cgaattcaaa gtggtcatgt gatgaatcca ttaaatatta attatcagta
     5581 tttgagggat acgaaagaat atcaagcaac ggagattatg acgcaacatg agccggattt
     5641 gccagaagcg gaaaagttat atttgacatt acacttactt tcaacaagtg tgcaatggac
     5701 tgatttgcaa gaatcagata gcatatcgaa tttaacgatg gccatcgctc aaatgattca
     5761 ccattttgaa caaatcactt ttattaacat tgaagataag gagaaattat cacagcaact
     5821 cttgttacat ttaacgcctg ctttttatag gattaaatat aacttaacgg atcgtgatga
     5881 attaataaat cctttacaag gaaattatca atccttattt catatggtga aacaatcatg
     5941 tcaatcgtta actgaatatt tcggaaaatc gttgcctgat aatgaaatag catatttaac
     6001 catgttgttc ggaggtagtt tgagacgtca agatgaaaac ttcgatggca agataaaagc
     6061 tattatcgtg tgtacacaag gcacgtcagt atcacaaatg atgttatacg agttgcgaaa
     6121 cttatttcca gaaattattt tcttagatgc gatttcactt agaacatttg aaaattacac
     6181 attagattat gacatcgtct tttcaccaat gtttgtccta acacataaaa aattatttat
     6241 cacaaaagta gctttatctg aaaatgagca acgaaagtta cgtaaagaag tgatgaagta
     6301 cattaataag gaatcggctg acattgataa ggaaataaac aagttaatgg cattaattga
     6361 acgcacaacg acagttaatg acattacaga actacgtgat ggtttagaag attttattgc
     6421 caattataat tcaatttcaa ccattaatgg atcgattgtc acacaaaata agacattaga
     6481 tttagctgac ttgataccgg caaggcacgt gaaaagaatg catcatgttg aaaatattga
     6541 tgaagctatt gctaaagcaa gtgatgtgtt agttgctaat cattttattg atattaaata
     6601 tattcatgag atgcaacagg tatttgatga ttcgtatatg gttatcatgc aaaatattgc
     6661 tattccacat gcatactctg aaaagcatgt acataaaaca gcgatgagta tgttgatatt
     6721 acaagaacca atatacatgt cagatggcac agcaatccat attattgtac ctattgctgc
     6781 tgttgataaa gtgacacact taagagcgtt actacaattg agagatgtgg cgcaagacaa
     6841 tgacgcaatt aagcgcatca tacaaagtcg caaaaattct gatgtaaatg agattttaaa
     6901 aaattattca aataaagaag cgagggaaaa tggatgggac agcaattagt gcataaagaa
     6961 aatataatgc tcaatttgtc ggcaactgat aaagaatccg tattgtcaca aatgtcagat
     7021 gtgttatttc aaaatgggtt cgtgaagtca acgtttaaag atgcagtcat cgacagagaa
     7081 aaagaatttg ctactggttt accaacgcat ctatgttcgg tcgctatacc gcatacagat
     7141 gtcgaacata ttaaccatag aacgataggt gtggctgttc tagaaaaaga agtgccgttt
     7201 attgaaatgg gaacacttga tcaacagaca gaagtgaaaa tcgtttttat gttagcaatg
     7261 gataaagtag atgatcaact taagctgtta caacagttga tgcaaatttt tcaaagtgaa
     7321 gaaaaattgg agcagattct acgaacgaaa gatgaaacaa ttttagcaac actaatcaat
     7381 gattatttgg aatataacta aaaattaatt ggaggaattg aaaatgaaac aagtattagt
     7441 agcgtgtggt gcaggtattg caacgtcaac agtagtaaat aatgcaattg aagaaatggc
     7501 aaaggaacac aatattaaag tagatattaa acaaatcaaa attacagaag ttggacctta
     7561 tgaagacact gcagatttat tagttacaac tgcaatgaca aaaaaagaat ataaattccc
     7621 agttatcaac gcacgtaatt tcttaactgg tattggtatt gaagaaacaa aacaacaaat
     7681 cttaacagag ttacaaaaat aacggaattg atatgtaacg tgggtcgata acatattaat
     7741 atatgcaaat tgcatataaa tgactatcac gacaaatgat tgttgatgac atagaaacgc
     7801 agtgactgta attcaaaagg actgcagtcg aatttagcaa gggttgtcgt tgaaatgact
     7861 gtaacgtcaa atgtaatcac aatcggaaag tatcgtgaca atgcatatat aacagggagg
     7921 gtttaaatat gagttacttc actgattttg taaggggatt tttagattta ggtgcaactg
     7981 ttattttacc ggttgtcata ttcttgcttg gcctattctt taggcagaaa attggagcgg
     8041 catttaggtc tggtttaaca ataggtgtgg cttttgtagg gattttctta gtcatcgatt
     8101 tattagttaa aaatttaggg ccagcagcac aagcgatggt taaaaattta ggcgtcagtc
     8161 tgaatgtgat tgatgtaggt tggccagcaa catcatctat cgcttgggca tcatctgtcg
     8221 cagcatttat tattccactc ggaatcatag ttaacgttgt attgctagta actaaagtga
     8281 caaagacgat gaatgtagat atttggaatt tttggcatta tacgtttaca gcagcaatgg
     8341 tttatgccgt atcaggcagt atttggcaag cgttattagc agcagttatt ttccaaatta
     8401 tctgtttgaa agtagcagat tggacagcac cgatgatgag tgagttcttt gatttaccag
     8461 gtgtatcgat tgctacagga agtacaattt cttatgcacc aggtatttac ttagttaaat
     8521 tgttacaaaa agtacccggt ctgaataagt tagatgctga tcctgaaaca attcaaaaac
     8581 gttttggcgc atttggagag tctatatttg tcggcttgat tttaggttta ggtattggtg
     8641 tgttagcagg ttacaaacct ggagacatca ttaatttagg aatgtcaatg gctgcagtaa
     8701 tggtattaat gcctagaatg gtaaaaatct taatggaagg tttaatgcca gtttcagagt
     8761 ctgcaagaac atggctaaat aaacgttttg gcgaacgtga aatttatatt ggattggatg
     8821 cggctgtagc attaggtcat ccagcagtta tttcgacagc attaatttta gtacctatca
     8881 ctgttttatt agccgttatt ttaccaggaa accaagtact accttttggt gacttagcaa
     8941 cgataccatt tgttgtcgcg tttattgttg gtgcagcaag aggaaacatt attcattctg
     9001 tcattgtggg tacgattatg attgcaattt cactatatat tgcaacagac gtagcaccca
     9061 ttttcacaga tatggcgaaa ggtacgaatg tacaaatgcc aaaaggttca tctgaaattt
     9121 caagtattga tcaaggtggt aatatcgtta actatcttat ctttaaacta tttagtctat
     9181 tcaattaaaa atcgaggtgt ttgtggtgaa agctttagta aaaacaagag aaggacatgg
     9241 caacttagaa cttcttgata aagaagttgc aacaccgcta gatgataaag taaagattaa
     9301 agtacattat gcaggaattt gtggcacaga tattcatact tatgaaggtc attataaagt
     9361 taattttcca gtgacattag gtcatgaatt ttctggtgaa atcgttgaag ttggagcaga
     9421 cgttaaagat tttaaagttg gtgaccgtgt cacatctgaa acgacattct atgtttgtaa
     9481 tgagtgtgaa tactgtaaat caaaagacta taatttatgc aaccatcgaa aaggtattgg
     9541 aacacaagtt gatggcgcat ttactaatta tgtcattgca cgtgaagaaa gtttgcatca
     9601 tattccagac gaagtatcgt atcagtctgc agctatgaca gaaccattag catgtgcata
     9661 tcatggcgtt tctaagattc aagtcaattc aggcgatgta gcagttgtaa tgggacctgg
     9721 gccaatcgga ttacttgtag cacaagtgtt aaaaagtaaa ggcgcaactg ttgtggtaac
     9781 tggattggac aatgacaaag tcagattaga taaagcagaa gcattgcaca tggattatgt
     9841 agtcaattta caacaaacag acttaaaaac gtatatcaat ggaattacag acggttacgg
     9901 tgcagatgtt gttgttgaat gttcaggtgc agttccagca gcacgacaag gtttggatat
     9961 tttacgcaaa aaaggtttct acagtcaaat aggtattttt aaggatgctg aaattccatt
    10021 tgatatggaa aaagtgattc aaaaagaaat aacagttgtt ggtagtagaa gtcaaaagcc
    10081 agcagattgg gaaccttcat tgcaacttat ggcggatggt ttagtaaatg ctgaagcttt
    10141 ggtgacaaaa atatatgata tttcgaaatg ggacgaggcg tatcaacatt taaaatccgg
    10201 cgaaggtatt aaagtattac ttaagccgct cgatttagat gaaaatgaag gagagaatta
    10261 atatggtaga atcaatgcta acttttatgc ttgggccatt aagacaaatc actgattttt
    10321 atatggaaca tttactcgta agtaattcca ttgtcattgc aggttatttt gcgacaggta
    10381 tttttaaaaa gaaaaaagtt gtgaattaaa tcaaatttga ggtgatttac aagtgaaagc
    10441 attgaaatta tatggcgtgg aagatttacg gtatgaggat aatgaaaagc cagtcattga
    10501 aagtgcgaat gacgttatta ttaaagtacg agcgactggc atatgtggtt cagacacgtc
    10561 acgatacaaa aaaatggggc catacattaa aggtatgcca tttggtcatg aattttcagg
    10621 tgtagtagat gccattggaa gtgatgttac gcatgttaat gtgggcgaca aagtgacagg
    10681 ttgcccagca ataccttgtt atcaatgcga gtattgttta aaaggtgaat atgcacgatg
    10741 tgaaaagtta ttcgtcattg gctcatatga acctggatcg ttcgcggaat atgtcaaatt
    10801 gccagcgcaa aatgttttaa aggttccaga caatgttgat tacattgaag cagcaatggt
    10861 tgagccatca gccgttgttg cgcatgggtt ttataaatcg aatatacaac ctggtatgac
    10921 tgttgcagta atggggtgtg gcagtatagg tttgttagct attcaatggg cacgaatatt
    10981 tggtgctgca catatcatcg ctatagatat agatgcgcat aaactagata ttgcaacatc
    11041 attgggcgca catcaaacaa tcaattcaaa agaagaaaat cttgagaaat tcatcgaaaa
    11101 tcattacgcc aatcaaatcg atttagctat agaatcatca ggtgctaaag ttacgattgg
    11161 tcaaatattg acgctaccta aaaaaggtgg cgaggtggta ttactcggaa taccatatga
    11221 tgatattgag attgatcgcg ttcattttga aaaaattctg cgtaacgagt tgacagtatg
    11281 tggctcttgg aactgtttgt ccagtaattt tccgggcaaa gagtggacgg caaccttaca
    11341 ttatatgaag acgaaagata ttaatgtaaa gcctattatt tctcattttt taccgttaga
    11401 aaaaggcccg gagacatttg ataaattagt taacaagaaa gaacgatttg ataaagtcat
    11461 gtttacgatt tattagtatg cacctttgag gacgaaaacg ctggtatagt tatagctatg
    11521 aaagtgcgaa tgccgtctgg tctatagata ctatcgaaat aattcatctt cgaatatacg
    11581 ttgataaata gccggtttac ttgtgtgaaa tatgcttgtg aatcggttgt tttgcatttt
    11641 gtatacttaa aatgagatgg caatatttga taatttttaa agtgaaaatc aagtacagcc
    11701 acttaataag ataaatttat tataatatat ggtaaaatga tggcagtaat aatgaatttg
    11761 aaaaagagta aacattaata cctttaacaa tttaatatcg tcagagttaa tgattaactg
    11821 catggcaaaa caacttagaa tggtcagtta caaaaataca tttttataaa aaattatcac
    11881 actattgtga caactatctt tggattaata aaagaggcaa gtgagcaata ggttaggctt
    11941 atgtgcgggc ataggtcagt aatgtataaa tggaaatgat gtaatgacag aatggaggac
    12001 aacatgattt atgcaggtat tttagcagga ggtattggtt cgagaatggg gaacgtgcca
    12061 ttaccaaaac aatttttaga tattgataat aaaccgattt taatccatac aattgagaag
    12121 ttcattttag tgagtgaatt taatgagatt attatcgcaa cgccagcaca gtggatttcc
    12181 catacacagg atattttaaa aaaatataac attacagatc aacgtgtcaa agtagttgca
    12241 ggtggtacgg atcgaaatga aacaattatg aacattatcg accatattcg caatgtaaat
    12301 ggaattaata atgatgatgt gattgtaact catgatgccg taagaccatt tttaactcaa
    12361 cgtattatta aagagaacat tgaagtagca gcaaaatatg gtgcagtaga tacagtcatt
    12421 gaagcaattg atacgattgt aatgtctaaa gataaacaga acatacacag tatccctgta
    12481 aggaatgaaa tgtatcaagg ccaaacacca caatcattta atattaaatt attacaagat
    12541 agttatcgcg ccttaagtag tgaacaaaaa gaaatcttat cagatgcatg taaaatcatt
    12601 gtcgaatctg gacatgcagt taaattggta cgtggagaac tatacaacat taaagtgaca
    12661 acaccgtatg atttaaaagt agcaaatgcc attattcaag gtgatattgc cgatgattaa
    12721 tcaagtatat caactcgttg caccgagaca gttcgacgtc acatataata atgttgatat
    12781 ttatggtaat catgtcatcg taagaccttt atacttgtct atttgtgcag ctgatcaaag
    12841 gtattacaca ggtcgaagag atgaaaatgt actacgcaaa aaattaccaa tgtcattagt
    12901 tcatgaagct gttggtgaag ttgtatttga tagtaaaggg gtatttgaaa aaggtacgaa
    12961 agtagtaatg gtaccgaata cacctacaga gcaacatcat attattgcgg agaattactt
    13021 agcctctagt tattttagat ctagtggtta tgatggtttt atgcaagact acgttgtgat
    13081 ggcacatgat cgtatcgttc cgctgcctaa tgacattgat ttgagtacga tttcatacac
    13141 agagttagtg tcagtaagtt atcatgctat acaacgattt gaacgtaaat ctataccttt
    13201 gaaaaccagc tttggtattt ggggtgatgg taacttaggt tatattactg ctattttgct
    13261 acgtaagttg tacccagaag ctaaaactta tgtatttggt aagacagact ataaattaag
    13321 tcatttttca tttgtagatg acatctttac agtaaatcaa ataccagatg atcttaaaat
    13381 tgatcatgca tttgaatgtg ttggaggtaa aggaagtcaa gttgcacttc aacaaatagt
    13441 tgaacatatt tcaccagaag gcagtattgc tttgttaggc gtaagtgaat tacccgtgga
    13501 agtgaataca cgattagtac ttgaaaaagg attaacgttg attggtagta gtcgaagcgg
    13561 ctctaaagat tttgagcaag ttgttgattt atatcgtaag tacccagaca tagttgaaaa
    13621 gttagcatta ttaaaaggac atgaaattaa tgtatgtacg atgcaagata tcgtccaagc
    13681 gtttgaaatg gatttatcga catcttgggg aaaaacagta ttgaaatgga cgatttaata
    13741 aaccgatggg ggaataacaa tgacaaaaac gaaacaagca atacatattg ataacatata
    13801 ctgggaacgt gttcagttat atattgaagg acatagtgaa ggtgtcgatt taacatcagg
    13861 acaatttgtt ctgaggaatt taaccgaaac aaaaacatta gaagcaaatg aaatgaaaat
    13921 agacggtaat acatttatat gtagattcaa cgtcgcaata ttagacgatg ggtattattt
    13981 accaatggat aaatatttat ttgtttatca tgaccagtta gagtatattg gacaacttaa
    14041 tccaaatatt attgatcaag cttatgcggc attaaatgaa gagcaaattg aagaatacaa
    14101 tgagctgact acacaaaatg gaaaagtgaa ctatttatta gcgtatgatg ctaaagtttt
    14161 ccgtaaaggt ggcgtatcac aacatacggt ctataccatt actccggaaa tagcaagtga
    14221 cgttaacgaa tttgtatttg atattgaaat caccttacct caagagaaat caggggtcat
    14281 tgcgacaagt gcacactggc ttcataaaca aggtcataaa gcttcatttg aaagtagaag
    14341 tttcttattt aaagctattt ttaatattac aaagttacta catattaaaa gaagcaaaac
    14401 aatattattc acatcagatt cgcgtccgaa tttatcaggg aatttcaagt atgtatatga
    14461 tgagttacta cgccaaaaag tagattttga ttatgatatt aaaacggtat ttaaggagaa
    14521 tattacggat agacgcaaat ggagagacaa gtttagattg ccatatttac ttggtaaggc
    14581 cgattatatt tttgttgatg atttccatcc attaatttat acggttcgct ttagaccatc
    14641 acaagaaatt attcaagtgt ggcatgctgt tggtgccttt aaaacagttg gctttagtcg
    14701 tacaggtaaa aaaggtggtc cgtttatcga ttcattaaac catcgtagtt acacgaaagc
    14761 atatgtttca tcagaaaccg atattccatt ttatgctgaa gcatttggaa ttagagaaga
    14821 aaatgttgta ccaacaggtg taccacgtac tgatgtacta tttgatgaag cttatgcaac
    14881 acaaattaaa caagagatgg aagatgaatt gccaattata aaaggtaaga aagttattct
    14941 attcgcaccg acatttagag gtaatggtca cggtacggca cattatccat tttttaaaat
    15001 tgattttgaa cgtttagcaa gatactgcga gaagcataat gcagttgtgt tattcaaaat
    15061 gcatccgttc gtaaaaaata gacttaatat ttcacgtgaa catagacaat actttatcga
    15121 tgtgtcagat catcgtgaag ttaacgatat tctctttgtt acagacttgt tgattagtga
    15181 ttattcatct ttaatatatg aatatgcagt atttaaaaag ccgatgattt tctatgcatt
    15241 tgacttagaa gattacatta cgacgcgtga tttctatgaa ccatttgaat catttgttcc
    15301 aggtaaaatt gtacagtcct ttgatgcatt aatggatgct ttggacaatg aagattatga
    15361 ggttgaaaaa gttgtgccat tcttagataa acattttaaa tatcaagatg gtcgctcaag
    15421 tgaacgttta gtcaaagatt tgtttagacg ctaatgttgg catatattac ttgccatgca
    15481 aatgagtcat aaggcatagt tttcaagaag ggttctgaca atgaagtgag caacatgtca
    15541 tgatgagaag caggactata caatgagaat aaccttttga tttttcatgc ataaggcgat
    15601 tcaatcaaaa gcaatcacct tccaactgaa ttgtcatttt gtaaaataaa ataatcgatc
    15661 caatcgttat ctaattcata aatgtgtaaa cacatacgtt atgaatggat aacgattttt
    15721 tgttatgtta aagtggtaca ttaatcatgt atttcgtatg ataattaacg acaagtgtaa
    15781 tggttaaatg tattttatga tgaaatgcta taataggcat ggttacaatg agcttgctca
    15841 tacatattaa tataattaca aaaacacgtc ggaggtacga catgattaaa aatacaatta
    15901 aaaaattgat agaacatagt atatatacga cttttaaatt actatcaaaa ttgccaaaca
    15961 agaatctaat ttattttgaa agctttcatg gtaaacaata cagcgacaac cccaaagcat
    16021 tatatgaata cttaactgaa catagcgatg cccaattaat atggggtgtg aaaaaaggat
    16081 atgaacacat attccaacag cacaatgtac catatgttac aaagttttca atgaaatggt
    16141 ttttagcgat gccaagagcg aaagcgtgga tgattaacac acgtacacca gattggttat
    16201 ataaatcacc gcgaacgacg tacttacaaa catggcatgg cacgccatta aaaaagattg
    16261 gtttggatat tagtaacgtt aaaatgctag gaacaaatac tcaaaattac caagatggct
    16321 ttaaaaaaga aagccaacgg tgggattatc tagtgtcacc taatccatat tcgacatcga
    16381 tatttcaaaa tgcatttcat gttagtcgag ataagatttt ggaaacaggt tatccaagaa
    16441 atgataaatt atcacataaa cgcaatgata ctgaatatat taatggtatt aagacaagat
    16501 taaatattcc attagataaa aaagtgatta tgtacgcgcc aacttggcgt gacgatgaag
    16561 cgattcgaga aggttcatat caatttaatg ttaactttga tatagaagct ttgcgtcaag
    16621 cgctggatga tgattatgtt attttattac gcatgcatta tttagttgtg acacgtattg
    16681 atgaacatga tgattttgtg aaagacgttt cagattatga agacatttcg gatttatact
    16741 taatcagcga tgcgttagtt accgactact catctgtcat gttcgacttc ggtgtattaa
    16801 agcgtccgca aattttctat gcatatgact tagataaata tggcgatgag cttagaggtt
    16861 tttacatgga ttataaaaaa gagttgccag gtccaattgt tgaaaatcaa acagcactca
    16921 ttgatgcatt aaaacaaatc gatgagactg caaatgagta tattgaagca cgaacggtat
    16981 tttatcaaaa attctgttca ttagaagatg gacaagcgtc acaacgaatt tgccaaacga
    17041 tttttaagtg ataacttaaa aacaataaaa aattataaat taattagtta agtgatataa
    17101 ataataaacg aaatgtttgc ttgtatgtta ttatttgtgt a
//
LOCUS       DAKTIO010000046        16740 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.046, whole genome shotgun sequence.
ACCESSION   DAKTIO010000046 DAKTIO010000000
VERSION     DAKTIO010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16740
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.046"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            168..1403
                     /gene="arcA"
                     /locus_tag="O0293_001125"
     CDS             168..1403
                     /gene="arcA"
                     /locus_tag="O0293_001125"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCY0280017.1"
                     /translation="MTDGPIKVNSEIGALKTVLLKRPGKELENLVPDYLDGLLFDDIP
                     YLEVAQKEHDHFAQVLREEGVEVLYLEKLAAESIENPQVRSEFIDDVLAESKKTILGH
                     EEEIKALFATLSNQELVDKIMSGVRKEEINPKCTHLVEYMDDKYPFYLDPMPNLYFTR
                     DPQASIGHGITINRMFWRARRRESIFIQYIVKHHPRFKDANIPIWLDRDCPFNIEGGD
                     ELVLSKDVLAIGVSERTSAQAIEKLARRIFENPQATFKKVVAIEIPTSRTFMHLDTVF
                     TMIDYDKFTMHSAILKAEGNMNIFIIEYDDVNKDIAIKQSSHLKDTLEDVLGIDDIQF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNDLLRQKGIKVIEISGSEL
                     VRGRGGPRCMSQPLFREDI"
     gene            1436..2446
                     /gene="argF"
                     /locus_tag="O0293_001126"
     CDS             1436..2446
                     /gene="argF"
                     /locus_tag="O0293_001126"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCY0280018.1"
                     /translation="MTEIQKPYDLKGRSLLKESDFTKAEFEGLIDFAITLKEYKKNGI
                     KHHYLSGKNIALLFEKNSTRTRAAFTVASIDLGAHPEFLGKNDIQLGKKESVEDTAKV
                     LGRMFDGIEFRGFSQQAVEDLAKFSGVPVWNGLTDDWHPTQMLADFMTIKENFGYLEG
                     INLTYVGDGRNNIAHSLMVAGAMLGVNVRICTPKSLNPKEAYVDIAKEKASQYGGSVM
                     ITDNIAEAVENTDAIYTDVWVSMGEESEFEQRINLLKDYQVNQQMFDLTGKDSTIFLH
                     CLPAFHDTNTLYGQEIYEKYGLAEMEVTDQIFRSEHSKVFDQAENRMHTIKAVMAATL
                     GS"
     gene            2537..3967
                     /gene="arcD"
                     /locus_tag="O0293_001127"
     CDS             2537..3967
                     /gene="arcD"
                     /locus_tag="O0293_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCY0280019.1"
                     /translation="MNESGDNKLSKSSLIGLVIGSMIGGGAFNIMSDMGGKAGGLAII
                     IGWIITAIGMISLAFVFQNLTNERPELDGGIYSYAQAGFGDFVGFISAWGYWFSAFLG
                     NVAYATLLMSAVGNFFPIFKGGNTLPSVIVASLLLWGVHFLILKGVETAAFINSIVTV
                     AKLIPILLVIICMIIAFNFDTFKTGFFSMTSEGVLPFSWASTMSQVKSTMLVTVWVFI
                     GIEGAVIFSSRAKNKKDVGSATVIGLISVLIIYFLLTVLAQGVILQNHISQLDSPSMA
                     QVLATIVGGWGSTLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNG
                     APVNALLITNILVQLFLISMLFTQSAYQFAFSLASSAILYPYMFSAFYQVKYTLEHRQ
                     QATTKQWTIGIIASIYAIWLIYAAGINYLLLTMLLYIPALLVYTIVQKNNQTRLIKSD
                     YILFMIIIVLAVIGLIKLLMGTINVF"
     gene            3984..4925
                     /gene="arcC"
                     /locus_tag="O0293_001128"
     CDS             3984..4925
                     /gene="arcC"
                     /locus_tag="O0293_001128"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCY0280020.1"
                     /translation="MKEKIVIALGGNAIQTTEATAEAQQTAIRCAMQNLKPLFDSPAR
                     IVISHGNGPQIGSLLIQQAKSNSDTTPAMPLDTCGAMSQGMIGYWLETEINRILTEMN
                     SDRTVGTIVTRVEVDKDDPRFDNPTKPIGPFYTKEEVEELQKEQPDSVFKEDAGRGYR
                     KVVASPLPQSILEHQLIRTLADGKNIVIACGGGGIPVIKKENTYEGVEAVIDKDFASE
                     KLATLIEADTLMILTNVENVFINFNEPNQQQIDDIDVATLKKYAAQGKFVEGSMLPKI
                     EAAIRFVESGENKKVIITNLEQAYEALIGNKGTHIHM"
     gene            5024..5728
                     /locus_tag="O0293_001129"
     CDS             5024..5728
                     /locus_tag="O0293_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCY0280021.1"
                     /translation="MTENFILGRNNKLEHELKALADYINIPYSILQPYQSECFVRHYT
                     KGQVIYFSPQESSNIYFLIEGNIIREHYNQNGDVYRYFNKEQVLFPISNLFHPKEVNE
                     LCTALTDCTVLGLPRELMAFLCKANDDIFLTLFALINDNEQQHMNYNMALTSKFAKDR
                     IIKLICHLCQTVGYDQDEFYEIKQFLTIQLMSDMAGISRETAGHIIHELKDEKLVVKD
                     HKNWLVSKHLFNDVCV"
     gene            6078..8777
                     /gene="clfB"
                     /locus_tag="O0293_001130"
     CDS             6078..8777
                     /gene="clfB"
                     /locus_tag="O0293_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfB"
                     /protein_id="HCY0280022.1"
                     /translation="MKKRIDYLSNKQNKYSIRRFTVGTTSVIVGATILFGIGNHQAQA
                     SEQSNDTTQSSKNNASADSEKNNMIETPQLNTTANDTSDISANTNSANVDSTTKPMST
                     QTSNTTTTEPASTNETPQPTAIKNQATAAKMQDQTVPQEANSQVDNKTTNDANSIATN
                     SELKNSQTLDLPQSSPQTISNAQGTSKPSVRTRAVRSLAVAEPVVNAADAKGTNVNDK
                     VTASNFKLEKTTFDPNQSGNTFMAANFTVTDKVKSGDYFTAKLPDSLTGNGDVDYSNS
                     NNTMPIADIKSTNGDVVAKATYDILTKTYTFVFTDYVNNKENINGQFSLPLFTDRAKA
                     PKSGTYDANINIADEMFNNKITYNYSSPIAGIDKPNGANISSQIIGVDTASGQNTYKQ
                     TVFVNPKQRVLGNTWVYIKGYQDKIEESSGKVSATDTKLRIFEVNDISKLSDSYYADP
                     NDSNLKEVTDQFKNRIYYEHPNVASIKFGDITKTYVVLVEGHYDNTGKNLKTQVIQEN
                     VDPVTNRDYSIFGWNNENVVRYGGGSADGDSAVNPKDPTPGPPVDPEPSPDPEPEPSP
                     DPDPDSDSDSDSGSDSDSGSDSDSESDSDSDSDSDSDSDSDSESDSDSESDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSESDSDSESDSESDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSRVTPPNNEQKAPSNPKGEVNHSNKVSKQHK
                     TDALPETGDKSENTNATLFGAMMALLGSLLLFRKRKQDHKEKA"
     gene            8893..9654
                     /locus_tag="O0293_001131"
     CDS             8893..9654
                     /locus_tag="O0293_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCY0280023.1"
                     /translation="MAYISLNYHSPTIGMHQNLTVILPEDQSFFNSDTTVKPLKTLML
                     LHGLSSDETTYMRYTSIERYANEHKLAVIMPNVDHSAYANMAYGHSYYDYILEVYDYV
                     HQIFPLSKKRDDNFIAGHSMGGYGTIKFALTQGDKFAKAVPLSAVFEAQNLMDLEWND
                     FSKEAIIGNLSSVKGTEHDPYYLLDKAVAEDKQIPKLLIMCGKQDFLYQDNLDFIDYL
                     SRINVPYQFEDGPGDHDYAYWDQAIKRAITWMVND"
     gene            9899..10354
                     /locus_tag="O0293_001132"
     CDS             9899..10354
                     /locus_tag="O0293_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCY0280024.1"
                     /translation="MSTNKNDYEHMLFYFAYKTFITTADEIIEKYGMSRQHHRFLFFI
                     NKLPGITIKSLLEILEISKQGSHATLQKLKEQGLIIEKVLETDRRVKKLYSTDKGDQL
                     IAELNKAQDELLQNIYQQVGSDWYDVMEALAKGRPGFDFIKHLKDEKES"
     gene            complement(10457..10567)
                     /locus_tag="O0293_001133"
     CDS             complement(10457..10567)
                     /locus_tag="O0293_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280025.1"
                     /translation="MLGAWLTRSWYFLNAKYKPPTNVKITANFFIIQTPF"
     gene            complement(10637..12061)
                     /locus_tag="O0293_001134"
     CDS             complement(10637..12061)
                     /locus_tag="O0293_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCY0280026.1"
                     /translation="MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVG
                     NTKNPKNVIFLVGDGMGPSFNTAYRYYKNDPHAKEMTPTAFDSYLKGTNRTYSNDPKQ
                     NITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDAT
                     PAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDG
                     YDLATNSKELSKSDKDKVLGLFADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGF
                     FLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKDTLVVATADHSTGGL
                     TIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFEFSQDDMKKVKKE
                     NKKLKRLLKKEKDEKSAEVETQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYG
                     PRSESFSGNIENTESAKIIFDIFK"
     gene            12259..12459
                     /locus_tag="O0293_001135"
     CDS             12259..12459
                     /locus_tag="O0293_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280027.1"
                     /translation="MILVMLSPLLIIFFIVLSILEERKRTKKKQLEKEKANTLNQNTN
                     DTESSNQEPSLQQDKEQKDNKG"
     gene            12485..13150
                     /gene="nsaR"
                     /locus_tag="O0293_001136"
     CDS             12485..13150
                     /gene="nsaR"
                     /locus_tag="O0293_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system response regulator NsaR"
                     /protein_id="HCY0280028.1"
                     /translation="MKILIVEDDFVIAESLASELKKWNYGVIVVEQFDDILSIFNQNQ
                     PQLVLLDINLPTLNGFHWCQEIRKTSNVPIIFISSRIDNMDQIMAIQMGGDDFIEKPF
                     NLSLTIAKIQALLRRTYDLSVANDSLTVKGCTLILDEAKVVYQEQNIQLSLTELQILK
                     LLFQNEDKYVSRTALIEKCWESENFIDDNTLAVNMTRLRKKLNTIGVNDFIITKKNVG
                     YKV"
     gene            13161..14048
                     /gene="nsaS"
                     /locus_tag="O0293_001137"
     CDS             13161..14048
                     /gene="nsaS"
                     /locus_tag="O0293_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system sensor histidine kinase NsaS"
                     /protein_id="HCY0280029.1"
                     /translation="MTFLKSITQEIAIVIVIFALFGLMFYLYHLPLEAYLLALGVILL
                     LLLIFIGIKYLSFVKTISQQQQIENLENALYQLKNEQIEYKNDVESYFLTWVHQMKTP
                     ITAAQLLLERDEPNVVNRVRQEVIQIDNYTSLALSYLKLLNETSDISVTKISINNIIR
                     PIIMKYSIQFIDQKTKIHYEPCHHEVLTDVRWTSLMIEQLINNALKYARGKDIWIEFD
                     EQSNQLHVKDNGIGISEADLPKIFDKGYSGYNGQRQSNSSGIGLFIVKQISTHTNHPV
                     SVVSKQNEGTTFTIQFPDE"
     gene            14156..14911
                     /locus_tag="O0293_001138"
     CDS             14156..14911
                     /locus_tag="O0293_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0280030.1"
                     /translation="MLLEVKHVKKVYGKGLNATTALNQMNLSVGAGEFVAIMGESGSG
                     KSTLLNLIASFDGLTEGDIIVDGAHLNNMKNKSKALYRQQMVGFVFQDFNLLPTMTNK
                     ENIMMPLILAGAKRKDIEQRVHQLAVQLHLEGFLNKYPSEISGGQKQRIAIARALVTK
                     PTILLADEPTGALDSKTSKALMMLFQEIHQLEQTILMVTHSNIDASYAERVIFIKDGR
                     LYHEIYRGEESQLAFQQRITDSLALVNGGSVNI"
     gene            14908..>16740
                     /locus_tag="O0293_001139"
     CDS             14908..>16740
                     /locus_tag="O0293_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0280031.1"
                     /translation="MKLRLLWHIVRRQFITQRLVIIPFILAVSVLFMIEYTLVSIGLN
                     SYIKQKNDFLVPFIIIANFFMALLTFIFIFYANHFMMSQRRKEFSIFMTLGMTKKSMR
                     LIVVMETILQFVIISVVSIAGGYLLGAIFFLFIQKIMGSEVATLRYYPFDSVAMFITL
                     IIIAVLMGMLLIFNLFSINFQRPITYQHRSDSSVISRWLRYVLIVIGSAALYLGYFIA
                     LQQDTTFGAFFKIWIVIGLVIIGTYAFFVGISEIIISILQQVSKVYYHPRYFFVVVGM
                     RVRLKMNAVSLATITLLCTFLIVTLTMTLTTYRDMNHTITKLITNDYDLSFSDNSKSQ
                     IERQQTIENIKRDIQGSVNAKDFKVYETTLFRASLEKNRAYYKLKQASDDIPIDFIGN
                     EGAIFSRQSVMITAFTAQDYNRYHQNKLHLDNQSIGMITNVPIFKKQSKVGLNNEVFK
                     VKQLDAHALNLMMIQDSMVLIVKDNNQLQKVKGFYAHEQKNSTISINFNVFGKTKLTT
                     SQIQKLSKKYDASINSKSEMLMVWDRLTGGLIFVGGVVSIVLVIGIFLMMYYKQVSEA
                     YANQHNYDIMKKLGLDNGRIAKITRNQMTFLFAIPITVALIHTLI"
BASE COUNT         5914 a         2505 c         3102 g         5219 t
ORIGIN      
        1 attttgaccc aaaatgcctt tatttatgta taaaaatcca ttattatgta ttgtataaca
       61 aaaagatatg aaattttcga ctttctttat gtgaatataa tcacatgtaa gcgtttgaag
      121 attgtctata ctctaaatga attcaaagat aaaaggagga aatagacatg acagatggtc
      181 caattaaagt aaatagcgaa attggagctt taaaaactgt gttacttaag cgtcctggaa
      241 aagaattaga aaatttagta cctgattatt tagatggatt actatttgat gatattccat
      301 atttagaagt agctcaaaaa gagcatgacc attttgcgca ggtgctaaga gaagagggtg
      361 ttgaagtact ttaccttgag aagttagcag ctgaaagtat tgaaaatcct caagtaagaa
      421 gtgaatttat tgatgatgta ttagcagagt ctaaaaaaac aatattaggt catgaagaag
      481 aaattaaggc attatttgcg acactttcta atcaagaact tgtagataaa ataatgtcag
      541 gggtacgtaa ggaagaaatt aatccgaaat gtacacatct agtagagtat atggatgata
      601 agtatccatt ctatttagat ccaatgccaa acctttattt tactagagat ccacaagcct
      661 caataggaca cggtataaca atcaatcgga tgttctggag agcacgacga cgagaatcaa
      721 tatttattca atatattgta aagcatcatc ctagatttaa agatgcgaat attccaatct
      781 ggttagatcg agattgccca ttcaatattg aaggcggcga tgaacttgtt ttatctaaag
      841 atgtcttggc tataggcgtt tcagaacgta catctgcaca agctattgaa aagttagcgc
      901 gacgtatttt tgaaaatccg caggcgacgt ttaaaaaagt agtagcaatt gaaattccaa
      961 ctagtcgaac ttttatgcac ttagatacag tatttacaat gatagattat gacaaattta
     1021 caatgcattc agccatttta aaggcagaag gcaatatgaa tatatttatt attgaatatg
     1081 atgacgtaaa taaagatatt gccatcaaac aatctagtca tttaaaagat actttagaag
     1141 acgtactagg tatagatgat atccaattca ttccaacagg aaatggtgat gtcattgatg
     1201 gtgctagaga gcaatggaat gatggctcaa atacattatg tataagacca ggcgttgtag
     1261 tgacttacga tagaaactat gtatcgaatg atttattgag acaaaaaggc attaaagtca
     1321 ttgaaatatc tggtagcgag ttggtacgtg gacgtggggg ccctagatgt atgagtcaac
     1381 cattattcag agaagacatt taacgtaata aattatagaa acgagggtga aaataatgac
     1441 agaaattcaa aaaccgtatg atttaaaagg cagatcatta ttaaaagaaa gtgattttac
     1501 caaagcagaa ttcgaaggac ttattgattt tgcaattaca ttaaaagagt ataagaaaaa
     1561 cggtattaag catcactact tatctggaaa aaatattgca ctactattcg aaaagaattc
     1621 gacgagaacg cgtgctgcgt ttacagttgc gtctattgat ttaggtgcgc atccagaatt
     1681 tttaggaaaa aatgatattc aattaggcaa aaaagaatct gtagaggata ctgcgaaagt
     1741 attaggtaga atgttcgatg gtattgaatt ccgtggtttt tcacaacaag ctgttgaaga
     1801 tttagcgaag ttctctggtg taccggtgtg gaatggatta acagacgatt ggcatcctac
     1861 acaaatgtta gctgatttta tgacaataaa agagaatttt ggatatctag aaggaataaa
     1921 cttaacttac gttggagatg gacgtaataa tattgcgcat tcattaatgg tagcaggtgc
     1981 tatgttaggt gttaatgtaa gaatttgtac acctaaatca ttaaatccaa aagaggcata
     2041 tgttgatatt gcaaaagaaa aagcgagtca atatggtggt tcagtcatga ttacggataa
     2101 tattgcagaa gcagttgaaa atacagatgc tatatataca gatgtttggg tatcgatggg
     2161 tgaagaaagt gaatttgaac aacgtattaa tttattaaaa gactatcaag tgaatcaaca
     2221 gatgtttgat ttaacaggta aagattcaac gatattctta cattgtttac cagcattcca
     2281 tgatacaaat acactttatg gacaagaaat ttatgaaaaa tatggattag ctgaaatgga
     2341 agttacagac caaatcttta gaagtgaaca ttcaaaagtg tttgatcaag ctgaaaatag
     2401 aatgcataca attaaggcag taatggcagc aacattgggg agttaatcac taaatggaac
     2461 gatatgaata tgatgtgtct gatgatataa gtgtcatgta cagacacctc atattggtat
     2521 taaaggagaa atgaatatga acgaatcagg agataacaaa ctcagtaaat cttctttaat
     2581 tggactagtt ataggatcca tgattggtgg cggtgcgttc aatataatgt ctgatatggg
     2641 cggtaaagcc ggtggattag ccattattat tggttggatt attacagcta taggaatgat
     2701 ttcattagcg ttcgtatttc aaaatttaac caatgaacgg ccggagctag acggtggtat
     2761 ttatagttat gctcaagcag gatttggcga ttttgtagga tttatcagtg cttggggata
     2821 ttggttctca gcgtttttag gcaatgttgc ctatgcaaca ctattgatgt cagcagtagg
     2881 taactttttc ccgattttta aaggaggcaa cacattacca agtgttattg tcgcctcgtt
     2941 actactctgg ggtgtccatt tcttgatttt aaaaggcgtt gaaacagcag catttatcaa
     3001 tagtattgtt actgttgcaa agttaatacc gattttactt gtaatcatat gcatgataat
     3061 tgcattcaat tttgacactt ttaaaacagg ctttttcagt atgacgtcag agggtgtatt
     3121 gccatttagt tgggcgagca caatgagcca agttaaaagt acgatgctag tgacagtttg
     3181 ggtgtttatc ggtatcgaag gtgcagtaat tttttctagt agagctaaaa ataaaaaaga
     3241 tgtaggtagt gccacggtta taggacttat atcagtttta attatctatt tcttattaac
     3301 tgtattagct caaggcgtga ttttgcaaaa tcatatttcg caattagatt cgccaagtat
     3361 ggcacaggtg cttgcaacta ttgtaggtgg ttggggatct acacttgtaa atattggttt
     3421 aattatttcg gtactaggtg catggttagg atggacactg cttgctggtg aattaccttt
     3481 cattgttgca aaagatggat tatttccaaa atggtttgct aaagaaaata aaaatggagc
     3541 acctgtaaat gcactgctta ttaccaatat attagtacaa ttatttttaa taagtatgct
     3601 atttacacag agtgcgtatc aatttgcatt ttcactagca tcaagtgcta ttttataccc
     3661 ttacatgttc agtgcatttt accaagttaa atacacttta gagcatcgac agcaagcaac
     3721 tactaaacaa tggacgattg gtatcatagc ctcaatttat gctatatggc ttatatatgc
     3781 agcaggtatc aattacttat tattgacgat gttactttat attccagctc ttcttgttta
     3841 tacaatcgtt caaaagaata atcagacacg tttgattaaa tcagactata ttctttttat
     3901 gattattatc gtacttgcag ttatcgggtt aattaagtta ttgatgggaa cgataaatgt
     3961 tttttaaaag gagcgacaaa aatatgaaag agaaaattgt cattgcatta ggcggtaatg
     4021 cgatacagac aacagaagca acagctgaag cacaacaaac agctattaga tgtgcgatgc
     4081 aaaaccttaa acctttattt gattcaccag cgcgtattgt catttcacat ggtaatggtc
     4141 cacaaattgg aagtttatta atccaacaag ctaaatcgaa cagtgacaca acgccggcaa
     4201 tgccattgga tacttgtggt gcaatgtcac agggtatgat aggctattgg ttggaaactg
     4261 aaatcaatcg cattttaact gaaatgaata gtgatagaac tgtaggcaca atcgttacac
     4321 gtgtggaagt agataaagat gatccacgat ttgataaccc aactaaacca attggtcctt
     4381 tttatacgaa agaagaagtt gaagaattac aaaaagaaca gccagactca gtctttaaag
     4441 aagatgcagg acgtggttat agaaaagtag ttgcgtcacc actacctcaa tctatactag
     4501 aacaccagtt aattcgaact ttagcagacg gtaaaaatat tgtcattgca tgcggtggtg
     4561 gcggtattcc agttataaaa aaagaaaata cctatgaagg tgttgaagcg gttatagata
     4621 aagattttgc tagtgagaaa ttagcaacgc tgattgaagc agatacctta atgattctta
     4681 cgaatgtaga aaatgtattt attaacttta atgaacctaa tcaacaacaa atcgatgata
     4741 ttgatgtagc aacactgaaa aaatacgcgg cacaaggtaa gtttgtggaa ggatcgatgt
     4801 tgccaaaaat agaagctgcg atacgatttg ttgaaagtgg ggaaaacaaa aaagttatca
     4861 ttaccaattt agagcaggca tacgaagctt tgattggtaa taaaggtaca cacattcaca
     4921 tgtagcactt tatcacgcga caaaacatta aatatgtttc tccgttgatt caaatgaaaa
     4981 agttgtctgc tgacactttg caaggtttga aggagtttaa cttatgacag aaaactttat
     5041 tttgggtaga aataataaat tagaacatga actaaaggca ttagcagatt acattaatat
     5101 accatatagt atattacaac catatcaaag tgaatgtttt gtcagacatt atacgaaagg
     5161 ccaagttatt tatttttcgc cacaagaaag tagcaatatt tactttttaa ttgaaggtaa
     5221 cattattaga gaacattaca atcaaaatgg agatgtatat cgttatttta ataaagagca
     5281 agtattattt ccaatcagta acttatttca tccgaaagag gttaacgaat tgtgtacagc
     5341 attaaccgat tgtacagttc ttggattgcc tagagaattg atggcctttt tgtgcaaagc
     5401 taatgatgat atatttttga cactttttgc attaataaat gataatgagc agcaacacat
     5461 gaactataac atggcattaa caagtaaatt tgctaaagat cgaattatca aattgatatg
     5521 ccatctatgt cagacagtag gatacgatca agatgaattt tatgaaatca aacagttttt
     5581 aactattcaa ctcatgagtg atatggctgg gatttcccgg gaaacagctg gtcatattat
     5641 tcatgaactt aaagatgaaa aacttgttgt taaagatcat aaaaattggt tagtaagcaa
     5701 acatttattc aatgatgtat gtgtttaata tacaatgtaa aatgaataag ttgaacatga
     5761 ggtctaacgt acatttatac gttaggcctt ttttgctagc atgatgaata atttaaaatg
     5821 ttagttaaat ttgattgttg aaattacagt aaaatttaag gtgatgaaaa atttagaact
     5881 tctaagtttt tgaaaagtaa aaaatttgta atagtgtaaa aatagtatat tgatttttgc
     5941 tagttaacag aaaattttaa gttatataaa taggaagaaa acaaatttta cgtaattttt
     6001 ttcgaaaagc aattgatata attcttattt cattatacaa tttagactaa tctagaaatt
     6061 gaaatggagt aatatttttg aaaaaaagaa ttgattattt gtcgaataag cagaataagt
     6121 attcgattag acgttttaca gtaggtacca catcagtaat agtaggggca actatactat
     6181 ttgggatagg caatcatcaa gcacaagctt cagaacaatc gaacgataca acgcaatctt
     6241 cgaaaaataa tgcaagtgca gattccgaaa aaaacaatat gatagaaaca cctcaattaa
     6301 atacaacggc taatgataca tctgatatta gtgcaaacac aaacagtgcg aatgtagata
     6361 gcacaacaaa accaatgtct acacaaacga gcaataccac tacaacagag ccagcttcaa
     6421 caaatgaaac acctcaaccg acggcaatta aaaatcaagc aactgctgca aaaatgcaag
     6481 atcaaactgt tcctcaagaa gcaaattctc aagtagataa taaaacaacg aatgatgcta
     6541 atagcatagc aacaaacagt gagcttaaaa attctcaaac attagattta ccacaatcat
     6601 caccacaaac gatttccaat gcgcaaggaa ctagtaaacc aagtgttaga acgagagctg
     6661 tacgtagttt agctgttgct gaaccggtag taaatgctgc tgatgctaaa ggtacaaatg
     6721 taaatgataa agttacggca agtaatttca agttagaaaa gactacattt gaccctaatc
     6781 aaagtggtaa cacatttatg gcggcaaatt ttacagtgac agataaagtg aaatcagggg
     6841 attattttac agcgaagtta ccagatagtt taactggtaa tggagacgtg gattattcta
     6901 attcaaataa tacgatgcca attgcagaca ttaaaagtac gaatggcgat gttgtagcta
     6961 aagcaacata tgatatcttg actaagacgt atacatttgt ctttacagat tatgtaaata
     7021 ataaagaaaa tattaacgga caattttcat tacctttatt tacagaccga gcaaaggcac
     7081 ctaaatcagg aacatatgat gcgaatatta atattgcgga tgaaatgttt aataataaaa
     7141 ttacttataa ctatagttcg ccaattgcag gaattgataa accaaatggc gcgaacattt
     7201 cttctcaaat tattggtgta gatacagctt caggtcaaaa cacatacaag caaacagtat
     7261 ttgttaaccc taagcaacga gttttaggta atacgtgggt gtatattaaa ggctaccaag
     7321 ataaaatcga agaaagtagc ggtaaagtaa gtgctacaga tacaaaactg agaatttttg
     7381 aagtgaatga tatatctaaa ttatcagata gctactatgc agatccaaat gactctaacc
     7441 ttaaagaagt aacagaccaa tttaaaaata gaatctatta tgagcatcca aatgtagcta
     7501 gtattaaatt tggtgatatt actaaaacat atgtagtatt agtagaaggg cattacgaca
     7561 atacaggtaa gaacttaaaa actcaggtta ttcaagaaaa tgttgatcct gtaacaaata
     7621 gagactacag tattttcggt tggaataatg agaatgttgt acgttatggt ggtggaagtg
     7681 ctgatggtga ttcagcagta aatccgaaag acccaactcc agggccgccg gttgacccag
     7741 aaccaagtcc agacccagaa ccggaaccaa gcccagaccc ggatccggat tcggattcag
     7801 acagtgactc aggctcagac agcgactcag gttcagatag cgactcagaa tcagatagcg
     7861 attcggattc agacagtgat tcagattcag acagcgactc agaatcagat agcgattcag
     7921 aatcagatag cgactcagat tcagatagcg attcagattc agatagcgat tcagattcag
     7981 atagcgattc ggattcagac agtgattcag attcagacag cgactcagaa tcagatagcg
     8041 actcagaatc agatagtgag tcagattcag acagtgactc ggactcagac agtgattcag
     8101 actcagatag cgattcagac tcagatagcg attcagactc agacagcgat tcagattcag
     8161 acagcgactc agattcagac agcgactcag actcagacag cgactcagat tcagatagcg
     8221 attcagactc agacagcgac tcagactcag acagcgactc agactcagat agcgactcag
     8281 attcagatag cgattcagac tcagacagcg actcagattc agatagcgat tcggactcag
     8341 acagcgattc agattcagac agcgactcag actcggatag cgattcagat tcagatagcg
     8401 attcggattc agacagtgat tcagattcag acagcgactc agactcggat agcgactcag
     8461 actcagacag cgattcagac tcagatagcg actcagactc ggatagcgac tcggattcag
     8521 atagcgactc agactcagat agtgactccg attcaagagt tacaccacca aataatgaac
     8581 agaaagcacc atcaaatcct aaaggtgaag taaaccattc taataaggta tcaaaacaac
     8641 acaaaactga tgctttacca gaaacaggag ataagagcga aaacacaaat gcaactttat
     8701 ttggtgcaat gatggcatta ttaggatcat tactattgtt tagaaaacgc aagcaagatc
     8761 ataaagaaaa agcgtaaata cttttttagg ccgaatacat ttgtattcgg tttttttgtt
     8821 gaaaatgatt ttaaagtgaa ttgattaagc gtaaaatgtt gataaagtag aattagaaag
     8881 gggtcatgac gtatggctta tatttcatta aactatcatt caccaacaat tggtatgcat
     8941 caaaatttga cagtcatttt accggaagat caaagcttct ttaatagcga tacaactgtt
     9001 aaaccattaa aaactttaat gttgttacat ggattatcaa gtgatgaaac gacatatatg
     9061 agatatacaa gcatagaaag gtatgcgaat gaacacaaat tagctgtgat tatgcccaat
     9121 gtggatcata gcgcatatgc taacatggca tatggtcata gctattatga ttatattttg
     9181 gaagtgtatg attatgttca tcaaatattt ccactttcca aaaagcgtga tgacaatttt
     9241 atagcaggtc actctatggg aggatatggc acaattaaat ttgcattaac acaaggggat
     9301 aaatttgcca aagctgtacc attatctgct gtgtttgaag cgcaaaattt aatggatcta
     9361 gagtggaatg atttttcaaa agaggccata attggcaatc tttcaagtgt taaaggaact
     9421 gaacatgatc cgtattactt gctagacaaa gctgtagctg aagataaaca aattccaaaa
     9481 ttgctcatta tgtgtggtaa acaagacttt ttatatcaag acaacttaga ttttatcgat
     9541 tatttatcac gcataaatgt tccttatcaa tttgaagatg gaccaggaga tcatgattat
     9601 gcatattggg atcaagcgat taagcgtgct ataacatgga tggtgaatga ttaattattt
     9661 cttggaaaat atgtggctgc attaaataca cagagtgaga gatacaaact atttacgcac
     9721 gactaacatt tctaagtgtt taaattattt ttgtattaat atgattggcg caatttgctg
     9781 atacacaaaa atgtttctcg tgaaacttag atttagctta tagttttatc atcatttgta
     9841 tgacttacat tataaatttt attataatga ggttaacgct ttgaaaggag tcatcatcat
     9901 gtcgaccaat aaaaacgatt atgagcatat gttgttttat tttgcatata aaacctttat
     9961 tactaccgct gatgaaatta tagagaagta tggtatgagt cgtcagcatc atcgtttttt
    10021 gttttttatc aataaattac ctggtattac tattaaatca ttactagaaa tattagaaat
    10081 ttctaaacaa ggatcacatg caacacttca aaaattaaaa gagcaaggtc tcattattga
    10141 aaaagtttta gagactgatc gacgtgtcaa aaaattatat tcgacggata aaggcgatca
    10201 actcattgct gaattgaaca aggcgcaaga tgaattattg caaaatatat atcaacaagt
    10261 cggttcggat tggtatgatg tgatggaagc attggctaaa gggcgacctg gctttgattt
    10321 tattaagcat ttgaaagatg aaaaagaaag ctagcatcag aaatgttaaa aatcttcgca
    10381 ttcttaaatt taaaaaatat gtcaaaaagt gtataataaa aacatataat ttaattgaac
    10441 tcagtttcaa cacatcttag aaaggagttt gaatgatgaa aaaattagca gttattttaa
    10501 cattagttgg cggtttatac ttcgcattta aaaaatacca agaacgtgtt aaccaagcac
    10561 ctaacattga gtactaaatt aaaccataaa aaattcccga acaccttgtt atagtgctcg
    10621 ggaatttttt tatgctttac ttgaatatat caaatattat ttttgcgctt tctgtatttt
    10681 cgatattacc actaaatgat tctgatctag gtccgtaagc gtaggtatta acatcctcgc
    10741 ctgtatgtcc atcggaagtc caccctgtat aagatttatc atttactggc ttctgaatag
    10801 cgtgttgtag ggcttttgtt tgcgtttcta cttctgcgga tttttcgtct ttttcttttt
    10861 taagtagtct ttttagcttt ttattctctt ttttaacctt tttcatatca tcttgtgaaa
    10921 attcaaatcc ataaccttca ttaataactt tttcagggtc ttcaccttta gccatttttt
    10981 ctgtcatata tgatccagag tgtttcatag atttaatcgg ttgaggattc cattcgtatc
    11041 ctttatcttt accaattgtt agaccaccag tagagtgatc tgctgttgca acgacaagtg
    11101 tatctttatg tttcttagca tattgaatgg cgtcatcgaa tgctttttca aaaccttcca
    11161 tttcagacat aacgcctgta atatcgttgg aatgcgctga tttatctata gaagcacctt
    11221 cgaccattaa aaagaatcct tttttattgc gctcaagctt actaagtgca ctttgttgca
    11281 tatcagctaa tgatggttcg tctttagaag catctattgc aagtggcata tttttatctg
    11341 caaacaaacc aagaacttta tctttatcag attttgataa ctccttactg ttcgtggcaa
    11401 ggtcgtaacc atcttttttg aattttttat ctaaattgcc attactttta ccgaaatatt
    11461 tagcgccgcc gcctaataaa acatcaactt tatgctttcc gttgatttta tctttataaa
    11521 attgtttagc gatttcgttt ttatcatctc tagaagtcac gtgtgcagca tatgctgctg
    11581 gtgttgcatc tgttaattca gctgttgaaa caagaccagt cgacttacct ttttcttttg
    11641 cacgttcaag caccgtcttt actttctgct tgttactgtc aacaccgatg gcaccattat
    11701 atgtcttatg accagaacta aaggctgttc cgccagctgc agaatcagta atattctgtt
    11761 ttgggtcatt tgaatatgta cgatttgtgc cttttaaata tgaatcaaaa gcagtagggg
    11821 tcatttcttt agcatgcgga tcatttttat aataacgata agctgtgtta aatgatggac
    11881 ccatgccatc gccaactaaa aagataacat tttttggatt tttagtatta ccaaccgcga
    11941 aactttcatc tttagaactt ttatcggatt gcgcaattgc aggtgtgaca gaactaaaaa
    12001 ccgttgacac gataataagg ttagcaactg caaattttgt ggctttttta actgataaca
    12061 taagacatcc tcctgagtat atgactatgt cttcagtgta aaagaggaat tttgagcaat
    12121 tatgtagttt tagttaaaaa tatgtaaaca gagtgattta gaataacaaa aaatgaatat
    12181 atatgacaat ttgttataga aagcgttaga atagaagcgt gtgaaaatat agaattaaat
    12241 ataatttgag gtggaaaaat gatactagta atgttatctc cattattaat catattcttt
    12301 atagtgttgt ctattttaga agagcgtaaa cgtacgaaga aaaagcaact cgagaaagaa
    12361 aaagcaaata cactaaatca aaatacaaat gacacggaaa gttcaaatca agagccgtca
    12421 ttgcagcagg ataaagaaca aaaagataac aaaggataat tcaattgaag gaagaagatt
    12481 atagatgaaa atattaattg ttgaagatga ttttgttata gcagagagtt tagcatctga
    12541 acttaaaaaa tggaattacg gtgttattgt cgttgaacaa tttgatgata tactgtctat
    12601 ctttaaccaa aatcaacctc agcttgtatt gctagatatt aatttgccaa cgttaaatgg
    12661 ttttcattgg tgtcaagaaa tccgaaaaac atctaatgtg ccaattatat ttattagttc
    12721 ccgtattgat aatatggacc aaattatggc aatacaaatg gggggagatg attttatcga
    12781 aaagccattt aacttgtcat taacgattgc caaaattcaa gcattattga gacgaactta
    12841 tgacttgtca gtagctaatg attcattgac ggttaaaggt tgtacattaa tattagatga
    12901 agcaaaagtc gtgtatcaag aacaaaacat acagctatct ttgactgaat tacaaatatt
    12961 aaagttatta tttcaaaatg aagataaata tgtaagtaga actgctttaa ttgaaaaatg
    13021 ttgggaatca gaaaacttca tagatgataa cacattagct gttaacatga cgcgcctgcg
    13081 taaaaaatta aatactattg gcgttaatga ttttatcatt acaaagaaaa atgtcggata
    13141 taaagtatag ggtgaatgca atgacctttc ttaaaagtat tactcaggaa atagcaatag
    13201 tcatagttat ttttgctttg tttggcttaa tgttttacct gtatcatttg ccattagaag
    13261 catatttact agcacttggc gttattttat tattattact catattcata ggtattaaat
    13321 atttaagttt tgtaaaaact ataagccaac aacaacaaat tgaaaactta gaaaatgcgt
    13381 tgtatcagct taaaaatgaa caaattgaat ataaaaatga tgtagagagc tactttttaa
    13441 catgggtaca tcaaatgaaa acacccatta ctgcagcaca actgttactt gaaagagatg
    13501 agcctaatgt tgttaatcgt gttcgtcaag aggttattca aattgataac tatacaagtt
    13561 tagcacttag ttatttaaag ttattaaatg aaacttctga tatttctgtc actaaaattt
    13621 cgattaataa tatcattcgc ccaattatta tgaaatattc aatacagttt attgatcaaa
    13681 aaacaaaaat ccattatgaa ccttgtcatc acgaagtatt aactgacgtt agatggacct
    13741 ctttaatgat agaacaatta ataaataatg cacttaagta tgcgagaggt aaagatatat
    13801 ggattgaatt tgatgagcaa tccaatcaat tacacgtaaa agataatggt atcggtatta
    13861 gtgaagcgga cttgcctaaa atatttgata agggctattc aggttataat ggccagcgcc
    13921 aaagtaactc aagtgggatt ggtttattta tcgtaaaaca aatttcaaca cacacaaacc
    13981 atcctgtttc agtcgtatct aaacaaaatg agggtacaac atttacgatt caatttccag
    14041 atgaataaaa actttcaata ttgtaagtat actagtaaca tttttttact aatttaaatg
    14101 ttattagtat ttttttgttt taatatagaa ctaacaaaga aatgaggtgc atgccatgtt
    14161 gctagaagtg aaacatgtaa aaaaggttta tggtaaaggt ttgaatgcta cgacagcact
    14221 taatcaaatg aatttatcag ttggagctgg tgaatttgtt gcaattatgg gtgagtctgg
    14281 gtcagggaag tctacactac taaatttaat tgcttctttt gatggactaa ctgaaggtga
    14341 cattattgtg gatggcgcac atttaaataa tatgaaaaat aaaagtaaag cattgtatcg
    14401 tcaacaaatg gtaggttttg tttttcaaga ttttaatctt ttaccaacaa tgacgaataa
    14461 agaaaatata atgatgccat taattttagc tggtgctaaa cgaaaagata tagaacaaag
    14521 ggtacatcag ttggcagtac aattacattt agagggattc ttaaacaagt atccttctga
    14581 aatctctggg ggtcagaagc aacgcattgc cattgcacgt gcattagtta ctaagccgac
    14641 gattttacta gccgatgaac ctacaggtgc acttgattca aaaacatcaa aggcattgat
    14701 gatgttattt caagagattc atcaattgga acagacaatt ttaatggtaa ctcattcaaa
    14761 tatcgatgcg tcttatgcag agcgagtcat ttttattaaa gatgggcgtc tatatcatga
    14821 aatatatcgt ggtgaagaaa gtcaattagc ttttcaacaa cgaataacag atagcttagc
    14881 acttgtgaat ggaggaagtg tcaatatatg aagttaagat tgttatggca catagtgcga
    14941 cgtcaattta ttacgcagcg acttgtaatc attccattca ttttagcggt aagtgtacta
    15001 ttcatgattg aatatacgct tgtgtcaatt gggttaaata gctacataaa acagaagaat
    15061 gacttcctag taccatttat tatcatagct aattttttta tggcgctttt aacttttatt
    15121 tttattttct atgcaaatca ctttatgatg tcacaaagac gaaaagagtt tagcattttt
    15181 atgacattgg gcatgaccaa gaaaagtatg cgtttaattg tagtgatgga aactatctta
    15241 caatttgtga taatttcagt cgttagtatt gccggcggat acttacttgg tgcgatattt
    15301 ttcttgttta tacagaaaat aatgggcagt gaagttgcga cgttaaggta ttatccattt
    15361 gactctgtag cgatgtttat tactttgatt atcattgctg tattaatggg catgctactt
    15421 atattcaact tgtttagtat taattttcaa cggccgataa cttatcaaca tcgttccgat
    15481 tctagtgtca tatcacgatg gttgcgttac gttttaattg ttataggaag cgcagcacta
    15541 tatttaggtt actttattgc attacaacaa gatacgacgt ttggtgcctt ttttaaaata
    15601 tggattgtca taggattagt tattatcggt acttatgcat tttttgtagg tataagtgaa
    15661 ataattatta gtatattgca gcaggtatca aaagtttact atcatccacg gtattttttt
    15721 gtggtagttg ggatgcgtgt acgtcttaaa atgaatgcag tcagtcttgc aacaatcact
    15781 ttgctgtgta catttttgat tgtaacgctc acaatgacat taacaaccta tcgtgatatg
    15841 aatcatacca ttacgaaatt gattacgaat gattatgatt tgtcatttag cgacaattct
    15901 aagtcacaaa tagaacgtca acaaacaatt gagaatatta aacgtgatat acagggttcg
    15961 gttaatgcaa aagacttcaa agtatatgaa acgacattat ttagagcttc tttagaaaag
    16021 aatcgtgctt attacaagtt aaaacaggct agtgatgata ttccaataga cttcattggt
    16081 aatgaaggcg cgatattttc aagacaatca gtaatgatta cagcttttac tgcacaagat
    16141 tataatcgat atcaccaaaa caaattacac ttagacaatc aaagtatagg tatgattacg
    16201 aatgtaccta tttttaaaaa gcagtctaag gtcggtctta ataatgaagt ctttaaagtg
    16261 aaacaacttg acgcacatgc gttaaatttg atgatgattc aagatagtat ggttttaatt
    16321 gttaaagata acaatcaatt acaaaaagta aaaggatttt atgcacatga acaaaagaat
    16381 tcgacaatat cgataaactt taatgtattt ggaaagacga aattaacaac atcacaaata
    16441 caaaagctat cgaaaaaata cgatgcgtca ataaattcta aatcagaaat gctaatggtt
    16501 tgggatcgtt tgacaggtgg actaatattt gtaggtggcg tagtatcaat tgtacttgtc
    16561 attggaatat ttctaatgat gtattataaa caagtctcag aagcctatgc aaatcagcac
    16621 aattatgaca ttatgaaaaa acttggttta gataatggaa gaatagcaaa gataacacga
    16681 aatcaaatga ctttcttgtt cgcaatacca ataacagtag cactgattca cacattaatt
//
LOCUS       DAKTIO010000047        16342 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.047, whole genome shotgun sequence.
ACCESSION   DAKTIO010000047 DAKTIO010000000
VERSION     DAKTIO010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16342
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.047"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            185..946
                     /gene="sufC"
                     /locus_tag="O0293_001140"
     CDS             185..946
                     /gene="sufC"
                     /locus_tag="O0293_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCY0280032.1"
                     /translation="MASTLEIKDLHVSIEDKEILKGVNLTINTDEIHAIMGPNGTGKS
                     TLSSAIMGHPSYEVTKGEVLLDGVNILELEVDERAKAGLFLAMQYPSEITGVTNADFM
                     RSAINAKREEGQEINLMQFIKKLDKNMDFLDIDKDMAQRYLNEGFSGGEKKRNEILQL
                     MMLEPKFAILDEIDSGLDIDALKVVSKGINQMRGENFGALMITHYQRLLNYITPDKVH
                     VMYAGKVVKSGGPELAKRLEEEGYEWVKEEFGSAE"
     gene            1044..2351
                     /gene="sufD"
                     /locus_tag="O0293_001141"
     CDS             1044..2351
                     /gene="sufD"
                     /locus_tag="O0293_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCY0280033.1"
                     /translation="MTTDILNISEEQLVDYSKAHNEPSWMTELRKKALKLTETLEMPK
                     PDKTKLRKWDFDSFKQHDVKGDVYQSLSQLPESVREIIDVDHSKNLVIQHNNTIAYTQ
                     VDDNASKDGVIVEGLADALMNHSDLVQKYFMKDAVTVDEHRITALHTALVNGGVFVYV
                     PKNVVVEHPVQYVVLHDDENASFYNHVIIVTEESAEVTYVENYLSNASGEGNQLNIIS
                     EVIAGANSNITYGSVDYMDKGFTGHIIRRGITEADASINWALGLMNEGSQIIDNTTNL
                     FGDRSTSSLKSVVVGTGEQKINLTSKIVQYGKETDGYILKHGVMKEHASSVFNGIGYI
                     KHGGTKSIANQESRVLMLSEHARGDANPILLIDEDDVQAGHAASVGRVDPDQLYYLMS
                     RGISQREAERLVIHGFLDPVVRELPIEDVKRQLREVIERKVSK"
     gene            2457..3707
                     /locus_tag="O0293_001142"
     CDS             2457..3707
                     /locus_tag="O0293_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCY0280034.1"
                     /translation="MNEVAEHSFDVNEVIKDFPILDQKVNGKRLAYLDSTATSQTPVQ
                     VLNVLEDYYKRYNSNVHRGVHTLGSLATDGYENARETVRRFINAKYFEEIIFTRGTTA
                     SINLVAHSYGDANVEEGDEIVVTEMEHHANIVPWQQLAKRKNATLKFIPMTADGELNI
                     EDIKQTINDKTKIVAIAHISNVLGTINDVKTIAEIAHQHGAIISVDGAQAAPHMKLDM
                     QEMNADFYSFSGHKMLGPTGIGVLFGKRELLQKMEPIEFGGDMIDFVSKYDATWADLP
                     TKFEAGTPLIAQAIGLAEAIRYLERIGFDAIHKYEQELTIYAYEQMSAIEGIEIYGPP
                     KDRRAGVITFNLQDVHPHDVATAVDTEGVAVRAGHHCAQPLMKWLNVSSTARASFYIY
                     NTKEDVDQLINALKQTKEFFSYEF"
     gene            3697..4161
                     /locus_tag="O0293_001143"
     CDS             3697..4161
                     /locus_tag="O0293_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HCY0280035.1"
                     /translation="MNFNNLDQLYRSVIMDHYKNPRNKGVLDNGSMTVDMNNPTCGDR
                     IRLTFDIEDGIIKDAKFEGEGCSISMASASMMTQAVKGHSLGEAMQMSQEFTKMMLGE
                     DYVITEEMGDIEALQGVSQFPARIKCATLAWKALEKGTVAKEGKAEGTTEEE"
     gene            4312..5709
                     /gene="sufB"
                     /locus_tag="O0293_001144"
     CDS             4312..5709
                     /gene="sufB"
                     /locus_tag="O0293_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCY0280036.1"
                     /translation="MAKKAPDVGDYKYGFHDDDVSIFRSERGLTENIVREISNMKNEP
                     EWMLDFRLKSLKLFYKMPMPQWGGDLSELNFDDITYYVKPSEQAERSWDEVPEEIKRT
                     FDKLGIPEAEQKYLAGVSAQYESEVVYHNMEKELEEKGIIFKDTDSALQENEELFKKY
                     FASVVPAADNKFAALNSAVWSGGSFIYVPKNIKLDTPLQAYFRINSENMGQFERTLII
                     ADEGASVHYVEGCTAPVYTTSSLHSAVVEIIVHKDAHVRYTTIQNWANNVYNLVTKRT
                     FVYENGNMEWVDGNLGSKLTMKYPNCVLLGEGAKGSTLSIAFAGKGQVQDAGAKMIHK
                     APNTSSTIVSKSISKNGGKVIYRGIVHFGRKAKGARSNIECDTLILDNESTSDTIPYN
                     EVFNDQISLEHEAKVSKVSEEQLFYLMSRGISEEEATEMIVMGFIEPFTKELPMEYAV
                     EMNRLIKFEMEGSIG"
     gene            5896..6033
                     /locus_tag="O0293_001145"
     CDS             5896..6033
                     /locus_tag="O0293_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HCY0280037.1"
                     /translation="MMVDKATTQIQHDCGIRVLVFIKLIESYIKYSLDNSILVRLRFV
                     V"
     gene            complement(6039..6353)
                     /locus_tag="O0293_001146"
     CDS             complement(6039..6353)
                     /locus_tag="O0293_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280038.1"
                     /translation="MYFVLAIFTIISASVSLGYSIQACASSHNINAYYALSRSLPLFL
                     LAIFSLVIHSAIFLITISIAMILVQFLDAIVGYKSEDVFKTYGPLATSVVNLILLIVF
                     LF"
     gene            6718..7758
                     /locus_tag="O0293_001147"
     CDS             6718..7758
                     /locus_tag="O0293_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCY0280039.1"
                     /translation="MIIAIIILIFISFFFSGSETALTAANKTKFKTEADKGDKKAKGI
                     VKLLEKPSEFITTILIGNNVANILLPTLVTIMALRWGISVGIASAVLTVVIILISEVI
                     PKSVAATFPDKITRLVYPIINICVIVFRPITLLLNKLTDSINRSLSKGQPQEHQFSKE
                     EFKTMLAIAGHEGALNEIETSRLEGVINFENLKVKDVDTTPRINVTAFASNATYEEVY
                     ETVMNKPYTRYPVYEGDIDNIIGVFHSKYLLAWSNKKENQITNYSAKPLFVNEHNKAE
                     WVLRKMTISRKHLAIVLDEFGGTEAIVSHEDLIEELLGMEIEDEMDKKEKEKLSQQQI
                     QFQQRKNRNVSI"
     gene            7772..8839
                     /locus_tag="O0293_001148"
     CDS             7772..8839
                     /locus_tag="O0293_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HCY0280040.1"
                     /translation="MWNKNRLTQMLSIEYPIIQAGMAGSTTPKLVASVSNSGGLGTIG
                     AGYFNTQQLEDEIDYVRQLTSNSFGVNVFVPSQQSYTSSQIENMNAWLKPYRRALHLE
                     EPVVKITEEQQFKCHIDTIIKKQVPVCCFTFGIPSEQIISRLKAANVKLIGTATSVDE
                     AIANEKAGMDAIVAQGSEAGGHRGSFLKPKNQLPMVGTISLVPQIVDVVSIPVIAAGG
                     IMDGRGVLASIVLGAEGVQMGTAFLTSQDSNASELLRDAIINSKETDTVITKAFSGKL
                     ARGINNRFIEEMSQYEGDIPDYPIQNELTSSIRKAAANIGDKELIHMWSGQSPRLATT
                     HPANTIMSNIINQINQIMQYK"
     gene            8906..9004
                     /locus_tag="O0293_001149"
     CDS             8906..9004
                     /locus_tag="O0293_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280041.1"
                     /translation="MRFYFTNLIKRKHQAEDRRKILIKQKHQTKVR"
     gene            9052..9900
                     /locus_tag="O0293_001150"
     CDS             9052..9900
                     /locus_tag="O0293_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCY0280042.1"
                     /translation="MINIGLTGWGDHYSLYEDLERQTDKLKTYAGHFPVVELDATYYA
                     IQPERNILKWIKETPDTFEFVVKIHQALTLHADYKTFADTRQELFDQFKNMLEPLHTQ
                     KKLAMVLVQFPPWFDCNAQNIKYILYVRQQLQAFPMCVEFRHQSWFSDAFKEQTLAFL
                     TEHQIIHAVVDEPQVKDGSVPLVNRITNEIAFVRYHGRNHYGWTKKDMSDQEWRDVRY
                     LYDYNEQELIDLAQKAQILAQKAKKVYVIFNNNSGGHAANNAKTYQRLLNIEYEGLAP
                     QQLKLF"
     gene            9913..10740
                     /locus_tag="O0293_001151"
     CDS             9913..10740
                     /locus_tag="O0293_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCY0280043.1"
                     /translation="MLLTITLLVLIGGLSAIIGSIVGIGGGIIIVPTMVYLGVEHGLL
                     HNITTQVAIGTSSVILIVTGLSSSLGYLKTKQVDIKNGSIFLFGLLPGSLLGSFISRY
                     LTFESFNLYFGIFLIFVAILLMVRNKIKPFKIFDKPKYEKTYVDAKGKTYHYSVPPLF
                     AFITTFLIGILTGLFGIGGGALMTPLMLIVFRFPPHVAVGTSMMMIFFSSVMSSIGHI
                     AQGHVAWGYAIILIISSYFGAKIGVKVNQSIKSDTVVTLLRTVMLLMGIYLIIRALI"
     gene            10767..12086
                     /locus_tag="O0293_001152"
     CDS             10767..12086
                     /locus_tag="O0293_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCY0280044.1"
                     /translation="MRLTIYHTNDIHSHLHEYERIKAYMAEHRPRLNHPSLYVDLGDH
                     VDLSAPITEATLGKKNVALLNEAKCDVATIGNNEGMTISHEALNHLYDEAKFIVTCSN
                     VIDESGHLPNNIVSSYIKDIDGVKILFVAATAPFTPFYRALNWIVTDPLESIKEEIEL
                     QRGKFDVLIVLSHCGIFFDETLCQELPEIDVIFGSHTHHYFEHGEINNGVLMAAAGKY
                     GNYLGEVNLTFEAHKVVHKTAKIIPLETLPEVETSFEEEGKTLMSNSVIQHPVVLKRS
                     MNHITEAAYLLAQSVCEYTHAQCAIINAGLLVKDIVKDEVTEYDIHQMLPHPINMVRV
                     RLFGVKLKEIIAKSNKQEYMYEHAQGLGFRGNIFGGYILYNLGYIHSTGRYYLNGEEI
                     EDDKEYVLGTIDMYTFGRYFPTLKELPKEYLMPEFLRDIFKEKLLEY"
     gene            12170..13087
                     /gene="lipA"
                     /locus_tag="O0293_001153"
     CDS             12170..13087
                     /gene="lipA"
                     /locus_tag="O0293_001153"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCY0280045.1"
                     /translation="MATKNEEILRKPDWLKIKLNTNENYTGLKKMMREKNLNTVCEEA
                     KCPNIHECWGARRTATFMILGAVCTRACRFCAVKTGLPNELDLNEPERVAESVELMNL
                     KHVVITAVARDDLRDAGSNVYAETVRKVRERNPFTTIEILPSDMGGDYDALETLMASR
                     PDILNHNIETVRRLTPRVRARATYDRTLEFLRRSKELQPDIPTKSSIMVGLGETIEEI
                     YETMDDLRANDVDILTIGQYLQPSRKHLKVQKYYTPLEFGKLRKVAMDKGFKHCQAGP
                     LVRSSYHADEQVNEAAKEKQRQGEAQLNS"
     gene            13217..13600
                     /locus_tag="O0293_001154"
     CDS             13217..13600
                     /locus_tag="O0293_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HCY0280046.1"
                     /translation="MIKVDQHYFELIENYRECFNEEQFIARYSDILDKYDYIVGDYGY
                     DQLRLKGFYKDSNKKAEMSKRFSNIQDYIFEYCNFGCPYFVLRHLSKQEVKKLIEEVH
                     PSDVIDDDNKLQDVKIKPTIQDTEH"
     gene            complement(13679..13945)
                     /locus_tag="O0293_001155"
     CDS             complement(13679..13945)
                     /locus_tag="O0293_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3055 domain-containing protein"
                     /protein_id="HCY0280047.1"
                     /translation="MIDMYLYDDNEESQVQFVGFVGEHSRYDLMLVHTNRHYGKTLVL
                     NMQTNKFGIIGTDDLKEEGYIAHILGVNAEEGDEITEYLNEVIH"
     gene            14043..14477
                     /locus_tag="O0293_001156"
     CDS             14043..14477
                     /locus_tag="O0293_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HCY0280048.1"
                     /translation="MYFVDKDKLTQKLAYLQALTDDYHESKHNHYAFERIAQMLIESS
                     VDIGNMIIDAFILRDPGNYKDVIDILELENVITKETQQAINKTVGIRKQFTYDYTALD
                     IKIIMPMFDDALPYYKQFITEVTTFLHQENVPVTAFGKGENQ"
     gene            14477..15256
                     /locus_tag="O0293_001157"
     CDS             14477..15256
                     /locus_tag="O0293_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HCY0280049.1"
                     /translation="MKQYKAYLIDLDGTMYMGTDEIDGAKQFIDYLNVKGIPHLYVTN
                     NSTKTPEQVTEKLREMHIDAKPEEVVTSALATADYISEQSPGASVYMLGGSGLNTALT
                     EAGLVIKNDEHVDYVVIGLDEQVTYEKLAIATLGVRNGATFISTNPDVSIPKERGLLP
                     GNGAITSVVSVSTGVSPQFIGKPEPIIMVKALEILGLDKSEVAMVGDLYDTDIMSGIN
                     VGMDTIHVQTGVSTLEDVQNKNVPPTYSFKDLNEAIAELEK"
     gene            15289..16248
                     /locus_tag="O0293_001158"
     CDS             15289..16248
                     /locus_tag="O0293_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HCY0280050.1"
                     /translation="MVKIVVSRKIPDKFYQQLSKLGDVVMWQKSLVPMPKDQFVTALR
                     DADACFITLSEQIDAEILAQSPNLKVIANMAVGYDNIDVESATANNVVVTNTPNVLTE
                     TTAELGFTLMLAIARRIVEAEKYVEADAWQSWGPYLLSGKDVFNSTIGIYGMGDIGKA
                     FARRLQGFNTNILYHNRSRHKDAEADFNATYVSFETLLAESDFIICTAPLTKETHHKF
                     NAEAFEQMKNDAIFINIGRGQIVDETALIDALDNKEILACGLDVLANEPIDHTHPLMG
                     RDNVLITPHIGSASVTTRDNMIQLCINNIEAVMTNQVPHTPVN"
BASE COUNT         5880 a         2332 c         3125 g         5005 t
ORIGIN      
        1 agagagcgga ttagagttgt actttagagt tattaaaaat aataaaaagg gtttaagttg
       61 tttatattaa ggtataagta agttataatt aactgaacgc attattacaa agtctttttg
      121 actacaaatt aaaattatta taaactagtt aagaaaactt tatattttac ggagggaata
      181 taaaatggca tcaacattag aaatcaaaga cctacatgtg tctattgagg ataaagaaat
      241 cttaaaaggt gttaacttga caattaacac tgatgaaata catgcgatta tgggaccaaa
      301 cgggacaggt aaatcaactt tatcatctgc aattatggga cacccaagct atgaagtaac
      361 taaaggagaa gtacttttag acggtgtaaa tattttagaa ttagaagttg atgaaagagc
      421 aaaagcagga ttattcttgg caatgcaata tccatcagaa attacaggtg ttacaaatgc
      481 tgatttcatg cgttcagcaa tcaatgcgaa acgtgaagaa ggacaagaaa tcaacttaat
      541 gcaatttatt aagaaattag ataaaaacat ggattttcta gacatagata aagacatggc
      601 acaacgttat ttaaatgaag gtttctcagg tggagagaag aaacgtaacg aaatcttaca
      661 attaatgatg ttagaaccta agtttgcaat cttagatgaa atcgattcag ggttagacat
      721 cgatgcatta aaagttgtat ctaaaggtat taaccaaatg cgtggggaaa actttggtgc
      781 attaatgatt acacactatc aacgattatt aaattacatt actcctgata aagtacatgt
      841 aatgtatgct ggtaaagtcg ttaaatctgg tggtccagaa ttagcaaaac gtcttgaaga
      901 agaaggatat gaatgggtta aagaagagtt cggttcagct gaataatctt attaatacag
      961 tatccatgag atgttcatct atatatgatg aaaatgaaca tttatacgaa atagtaaatt
     1021 tcatcaagta ggaggaaaaa gttatgacaa ctgatatttt gaacatttct gaagaacaac
     1081 ttgttgatta ttctaaagcc cacaatgaac cttcttggat gacagaatta cgtaaaaaag
     1141 ctttgaaatt aacagaaact ttagaaatgc caaaacctga taaaacaaaa ttaagaaaat
     1201 gggattttga ttcttttaaa caacacgatg taaaaggtga tgtttatcaa tctttatcac
     1261 aattacctga gtcagtaaga gaaattattg acgtagatca ttctaaaaac ttagtaattc
     1321 aacataataa tacgattgcg tacacacaag ttgatgataa tgcatcgaaa gatggcgtta
     1381 tcgttgaagg tttagcagac gctcttatga accatagtga tttagtacaa aagtacttta
     1441 tgaaagatgc agtaacagta gatgaacatc gtatcacagc gctacacacg gcattagtta
     1501 atggtggcgt atttgtttat gttcctaaaa atgtagttgt agaacatcca gtacaatacg
     1561 ttgtgttgca cgacgacgaa aatgcaagct tttataacca tgttatcatc gttactgaag
     1621 aaagcgccga agtcacatat gttgaaaatt acttatcaaa tgcatctggt gaaggaaatc
     1681 aattaaatat tatttctgaa gtgattgctg gtgcaaattc aaatatcaca tatggctcag
     1741 tggactatat ggataaaggc tttacaggtc atatcattcg acgtggtatt actgaagcgg
     1801 atgcctcaat taattgggca ctaggtttaa tgaatgaggg tagccaaatt attgataata
     1861 caacaaattt atttggtgat cgttcaacaa gttcacttaa atcagtagtt gtaggtacag
     1921 gcgaacaaaa aattaatcta acatctaaaa tcgtacaata tggtaaagaa acagatggtt
     1981 atatccttaa acatggtgtt atgaaagaac atgcatcgtc tgtatttaat ggtatcggct
     2041 acattaagca tggtggaact aaatcaattg ctaatcagga atcacgtgta ttaatgttat
     2101 ctgaacatgc tcgtggtgac gcgaatccta ttttattaat tgatgaagat gatgtacaag
     2161 ctggtcatgc tgcatcagta ggtcgtgttg atccagatca actttactat ttaatgagtc
     2221 gtggtatttc tcaaagagaa gcggaacgtc ttgttataca tggtttctta gatccagtag
     2281 tacgtgaatt acctatcgaa gacgttaaac gtcaattgag agaagtaatt gaacgcaaag
     2341 tttctaaata atattttgaa aataaaagtt tgtaatagat atagactgtc gatattggta
     2401 taagactaat acaacgtcag tatttaaatg attaggattt ttatttaaga aaggtcgtga
     2461 atgaagtggc cgaacactca tttgacgtta atgaagtaat caaggatttt ccgatattag
     2521 atcaaaaagt caatggcaaa cgtttagcat atcttgattc aacagcgaca agtcaaacgc
     2581 ctgtgcaagt gttaaatgtt ttagaagatt actacaagcg ttataattca aacgttcatc
     2641 gtggtgttca tacattagga tcattggcaa ctgatggtta tgaaaatgcc cgtgaaaccg
     2701 ttcgtcgttt tattaatgcg aagtattttg aagaaatcat ttttacacgc ggaacaactg
     2761 cgtcgattaa ccttgtagca catagctatg gtgatgcaaa tgttgaagag ggcgatgaaa
     2821 ttgttgtcac tgaaatggaa catcatgcca atattgttcc ttggcaacag ttagcaaagc
     2881 gtaaaaatgc gacattgaaa tttataccaa tgacagctga cggtgaatta aacatcgagg
     2941 atattaagca aacgattaat gataaaacaa agatcgttgc tattgcacat atatctaatg
     3001 tgctcggtac aattaatgat gttaaaacca ttgcagaaat agctcatcaa catggtgcaa
     3061 ttatcagtgt tgatggggcg caagcagcac cacatatgaa acttgatatg caagaaatga
     3121 atgctgattt ttatagtttt agtggtcata aaatgcttgg accaacaggt attggcgtat
     3181 tatttggtaa acgtgagtta ctacaaaaaa tggaaccgat tgagttcggt ggcgacatga
     3241 ttgattttgt aagtaagtat gatgcaacat gggctgattt acctactaaa tttgaggcgg
     3301 gtactccatt aattgctcaa gcaattgggc ttgcagaagc tattcgctat ttagaacgca
     3361 taggttttga tgcaattcat aaatatgaac aagaattaac gatatatgct tatgagcaaa
     3421 tgtctgcaat tgaaggaatt gaaatttatg gcccgccaaa ggatcgtcgt gcaggtgtaa
     3481 taacgtttaa tttacaagat gtacatccac acgatgttgc tacagccgta gatacagaag
     3541 gtgtagcggt tagagctggg catcattgtg cgcaaccgtt aatgaaatgg ttaaatgtgt
     3601 cttcaacagc tagagcgagt ttttatatat acaacacgaa agaagacgtt gatcagttaa
     3661 taaatgcctt gaaacaaacg aaggagtttt tctcttatga attttaataa tctagatcaa
     3721 ttatatagat ctgtcattat ggatcattat aaaaatccta gaaataaagg tgtattagat
     3781 aacgggtcta tgacagtaga tatgaataac ccgacatgcg gtgaccgtat acgactaaca
     3841 tttgatatag aagacggcat tataaaagat gctaagtttg aaggtgaagg ttgttcgatt
     3901 tcaatggcaa gtgcatcgat gatgacacaa gctgttaaag gtcattcact tggagaagca
     3961 atgcaaatga gccaagaatt tacgaaaatg atgcttggtg aagactatgt gattacagaa
     4021 gaaatgggag atattgaagc attgcaaggt gtatctcaat tcccagctcg tattaaatgt
     4081 gccacattag cttggaaagc attggaaaaa ggtactgttg ctaaagaagg taaagcagaa
     4141 ggtacgactg aagaagaata gaatgctgtt aatcatagat aattttgata ttagacatat
     4201 aaaagtataa aaatttttat aagatgtcat gtcattgtta taatatggtt tacatcatga
     4261 attaaaaact tacgcacgcc gttgtaaata tatttttaag gagtgattga aatggctaaa
     4321 aaagcacctg atgttgggga ttataaatat ggattccacg acgatgatgt gtccattttc
     4381 agatcagaac gtggtttaac tgagaatatc gttagagaaa tttctaacat gaaaaatgag
     4441 ccggaatgga tgttagattt ccgtcttaaa tcattaaaat tgttttataa aatgccaatg
     4501 cctcaatggg gtggcgactt atcagaattg aatttcgatg acattactta ctatgtaaag
     4561 ccttcagaac aagctgaacg ttcatgggat gaagtgccag aagaaattaa aagaactttc
     4621 gataaattag gaattcctga agctgaacaa aaatatttag ctggtgtttc tgctcaatat
     4681 gaatctgaag ttgtttacca taatatggaa aaagaacttg aagaaaaagg tattatcttt
     4741 aaagatacag atagtgcttt acaagaaaat gaagaattat tcaaaaaata ctttgcttct
     4801 gtagtacctg cagcagataa caaatttgcg gcgttaaact cagcagtatg gtcaggtggt
     4861 tcgttcattt atgtacctaa aaatatcaaa ctagatacgc cactacaagc ttatttccgt
     4921 attaactctg agaacatggg tcaatttgaa cgtacattaa tcattgctga tgaaggtgct
     4981 tctgtacatt acgtagaagg ttgtactgca ccagtttata caactagttc tttacactct
     5041 gctgttgtgg aaatcattgt gcataaagat gcgcacgttc gttatactac gattcaaaac
     5101 tgggcgaaca atgtatacaa tttagttaca aaacgtactt ttgtttatga aaacggaaat
     5161 atggaatggg tagatggtaa cttaggttct aagttaacga tgaaatatcc aaactgtgtt
     5221 cttttaggtg aaggtgcaaa aggtagtaca ttatctattg catttgctgg taaaggacaa
     5281 gttcaagatg ccggtgctaa aatgattcat aaagcaccta atacatcttc tacaattgtt
     5341 tctaaatcta tttctaaaaa tggtggtaaa gttatttatc gcggtattgt tcattttgga
     5401 cgtaaagcaa aaggtgctcg ttcaaatatt gaatgtgata cattaatctt agataacgaa
     5461 tcaacatcag atacaattcc atataacgaa gtattcaacg atcaaatatc attagaacat
     5521 gaagccaagg tttcaaaagt ttctgaagaa caattattct atctaatgag tcgtggtatt
     5581 tctgaagaag aagcgacaga aatgattgtt atgggattca tcgaaccatt tacaaaagaa
     5641 cttccaatgg aatacgcggt cgaaatgaac cgtttaatca agttcgaaat ggaaggtagt
     5701 attggatagc tttaaaccgc gttgttaagc cattcttgac ttccgaaaat ggctattgat
     5761 accattttga tactgaatat aacaaaaagc cacattactg tggctttttt tgttttataa
     5821 ctaaatcgga ttgatagata agctttgtac ttatttatat cagttcgatt ttttgattgg
     5881 tgtaaaaaat aatcattgat ggtggataaa gcgacaacac aaatacaaca tgattgtggc
     5941 attagagtgc tggtctttat taaattaatt gaaagctaca tcaaatattc tttagataat
     6001 tcgatattag ttcgattaag attcgttgta taagtgagtt aaaataagaa aactattaat
     6061 aatattaagt tcactacaga tgttgctaat ggaccataag ttttaaagac atcttcactt
     6121 ttataaccaa caatcgcatc taaaaattga actaagatca ttgcaatgga tatagttatc
     6181 aaaaatatag cactatgaat gactaaagaa aaaatagcta ataaaaataa aggtaagctt
     6241 cgactaagtg cataatatgc atttatatta tggctagatg cacatgcttg aattgaataa
     6301 cctaaactta cactggcact gattattgta aatattgcta aaacaaaata catgttaatc
     6361 cttctttcta tatttggata taaacaagta cttgtctaaa gttatttaaa agataattag
     6421 aataaattta tgagaaactg gttgttatca ttataatggt ttcaaatgat tataactatg
     6481 tcataaactg aatttgttga aatttttcat tatgcaaatt tattaataac aaacagctcg
     6541 aactatagca tcattttact aatgaatgca ttaaagtaac tatgactaaa aatgcatatt
     6601 aattatcatt attaagacta ttatatataa tgaattttaa ctggtttatt aaacgagaac
     6661 gtcgggaatt aagtaactac aataaaaata agatatgaca ataaggagac tacacgcgtg
     6721 atcattgcca taattatatt gatatttatt tcgtttttct tttcaggaag cgagacggca
     6781 ttaacggctg ccaataaaac aaaatttaaa actgaagctg acaaaggtga taaaaaagca
     6841 aaaggcattg taaagttact tgaaaaacca agtgagttta ttacaacgat tctaattggg
     6901 aataatgtcg cgaatatttt attaccaaca cttgttacaa ttatggcttt acgttggggg
     6961 attagcgttg gtattgcatc agctgtttta acagttgtta tcattttgat ctccgaagtg
     7021 attcccaagt ctgtcgctgc aacatttcca gataaaataa caaggcttgt atatccaatt
     7081 attaatattt gtgtcattgt gttccgtcct atcacattac ttttaaataa gttgacggac
     7141 agtattaatc gaagtttatc taagggccaa cctcaagaac atcaattttc aaaagaagaa
     7201 tttaaaacaa tgttagcaat tgctggacat gaaggtgctt taaatgaaat tgagacgagt
     7261 aggttggaag gtgtcattaa ttttgaaaat ttaaaagtaa aagatgtaga tacaacacct
     7321 agaattaatg tgacggcatt tgcttcaaat gcgacatacg aagaagttta tgaaacggtt
     7381 atgaataagc catacactag atatccagtg tacgagggag atattgataa cattattggg
     7441 gtgtttcatt ctaaatatct gttggcttgg agtaataaaa aagaaaatca aattacaaac
     7501 tattcagcta agccattatt tgtgaatgaa cacaataaag ctgaatgggt attacgtaag
     7561 atgactattt ctagaaaaca tttagcaatt gtgttggacg aatttggtgg tactgaagcg
     7621 atagtgtcac atgaagactt aattgaagaa ttattaggta tggaaattga agatgagatg
     7681 gataaaaagg aaaaagaaaa actttctcaa cagcaaattc aatttcaaca acggaaaaat
     7741 cgcaacgtat ctatataagg agcgaacagc tatgtggaat aagaatcgac ttactcaaat
     7801 gttaagtatt gaatatccaa ttatacaagc aggtatggca ggaagtacga caccgaaatt
     7861 agttgcatca gtaagtaaca gtggtgggtt aggcacaata ggcgcaggtt actttaatac
     7921 gcagcaattg gaagatgaaa tagattatgt acgccaatta acgtcaaatt cttttggcgt
     7981 aaatgtcttt gtaccaagtc aacaatcata taccagtagt caaattgaaa atatgaatgc
     8041 atggttaaaa ccttatcgac gcgcattaca tttagaagag ccggttgtaa aaattaccga
     8101 agaacaacaa tttaagtgtc atattgatac gataattaaa aagcaagtgc ctgtatgttg
     8161 ttttactttt ggaattccaa gcgaacagat tataagcagg ttgaaagcag cgaatgtcaa
     8221 acttataggt acagcaacaa gtgttgatga agctattgcg aatgaaaaag cgggtatgga
     8281 tgctatcgtt gctcaaggta gtgaagcagg tggacatcgt ggttcatttt taaaacctaa
     8341 aaatcaatta cctatggttg gaacaatatc tttagtgcca caaattgtag atgtcgtttc
     8401 aattccggtc attgccgctg gtggaattat ggatggtaga ggagttttgg caagtattgt
     8461 cttaggtgca gaaggggtac aaatgggcac cgcattttta acatcacaag acagtaatgc
     8521 atcagaacta ctgcgagatg caattataaa tagtaaagaa acagatacag tcattacaaa
     8581 agcgtttagt ggaaagcttg cacgcggtat caacaatagg tttatcgaag aaatgtccca
     8641 atacgaaggc gatatcccag attatccaat acaaaatgag ctaacaagta gcataagaaa
     8701 agccgcagca aacatcggcg acaaagagtt aatacatatg tggagtggac aaagcccgcg
     8761 actagcaaca acgcatcccg ccaacaccat catgtccaat ataatcaatc aaattaatca
     8821 aatcatgcaa tataaataat cgaccgcaat ccacaaaagc acaagcaccc ccaaacatta
     8881 ttttagtgct tgccattttt gtggattgcg tttctatttt acaaatttaa tcaaacgaaa
     8941 acatcaagct gaagatcgcc gaaagatttt aatcaagcaa aaacatcaaa ctaaagttcg
     9001 ctgaaatgat tatgataaaa gttatatggt atgatgacat tggtgatata tatgataaac
     9061 atcggattaa caggttgggg tgatcactat tcattatatg aagatttaga acgccaaacc
     9121 gataaactta aaacatatgc tggacatttt ccggttgtcg aattagatgc gacatactat
     9181 gcgatacaac cggaaagaaa tatattgaaa tggataaaag aaacgcctga tacatttgaa
     9241 tttgtggtca aaattcatca agcactcaca ttgcatgcag actacaaaac atttgcagat
     9301 acaaggcaag aactatttga tcaatttaag aatatgttag agcccttaca tacacagaaa
     9361 aaattagcaa tggtattggt tcaatttccg ccatggtttg actgcaatgc acaaaatatc
     9421 aaatatattt tgtatgtaag acagcaatta caagcatttc caatgtgtgt agaatttagg
     9481 catcaatcat ggtttagtga tgcatttaaa gaacaaacat tggcattttt aacagaacat
     9541 caaatcattc atgcagtagt tgatgaacca caagtgaaag atggcagtgt acctttagtc
     9601 aatcgaatca caaatgaaat tgcgtttgta cgttatcatg gacgtaatca ttacggttgg
     9661 actaagaaag atatgtcaga tcaagaatgg cgcgatgtac gctatttata tgattataat
     9721 gagcaagaat taatagactt ggcacaaaag gcacaaatat tagcacaaaa agctaagaaa
     9781 gtttacgtca tatttaacaa taattctggt ggtcatgcag caaataatgc caaaacatat
     9841 cagcgattat tgaatataga atatgaaggg ttagcaccac aacaattaaa attattttaa
     9901 gaggcgacga ctatgttatt aacaattaca ttattagttt taatcggagg tttgtcagcg
     9961 attatagggt ctatcgtagg cattggaggc ggtattatta tcgttccaac aatggtttac
    10021 ctcggtgttg aacatggatt actacataat attacaacac aagtagcgat agggacgtct
    10081 tcagtcattc taattgtgac aggactttct tcatcacttg gatatttaaa aacaaaacaa
    10141 gttgatatta aaaatggttc catcttttta tttggactat taccaggttc attgcttggg
    10201 tccttcatta gtagatattt aacatttgag tcatttaatt tatattttgg tatcttttta
    10261 attttcgtag ccattttatt aatggtaaga aataagatta aaccgtttaa aattttcgat
    10321 aaacccaagt atgaaaagac ttatgtagac gctaaaggta aaacatatca ttatagtgtt
    10381 ccaccattgt ttgcttttat tacaacgttt ttaattggta tattgacagg tttatttggt
    10441 attggaggtg gcgcactaat gacgccacta atgcttattg tatttagatt tccacctcat
    10501 gtagctgttg gaacaagtat gatgatgatt ttcttttcaa gtgtcatgag ttctataggg
    10561 cacattgctc aaggtcacgt agcttggggt tatgcaatca ttttaattat ttctagttat
    10621 tttggtgcga aaatcggtgt caaagtgaat caatcaatta agtcagatac ggtagtaaca
    10681 ttattgagaa cagtaatgtt gttaatgggt atatatttaa ttattcgtgc gttgatttaa
    10741 tacaacttta aaaggaggac gtcaatttga ggcttacaat ttatcatacg aacgatattc
    10801 atagtcattt acatgaatac gaacgcatta aagcatatat ggcagaacat cggccacgac
    10861 ttaatcatcc ttctttatat gttgatctag gtgatcatgt agatttatcc gcacctataa
    10921 ctgaagcaac tttaggtaaa aagaatgtgg cattactaaa tgaagcaaaa tgtgatgttg
    10981 caacaatcgg taataatgaa gggatgacca tttcacacga agctttaaat cacctttacg
    11041 acgaagcaaa atttatagtg acatgtagca atgttataga tgaatcaggt catttaccaa
    11101 ataatatcgt ttcttcttat attaaggaca tagacggtgt gaaaatacta ttcgttgcag
    11161 cgacagcacc ttttacccca ttttatcgtg cactaaattg gattgttacc gatccacttg
    11221 aatctataaa agaagaaatt gaacttcaac gaggtaaatt tgatgtatta atcgtgctaa
    11281 gtcattgtgg cattttcttc gatgaaacat tatgccaaga attgcctgaa attgatgtca
    11341 tttttggtag tcatacgcat cattattttg aacatggtga aatcaataat ggtgtactga
    11401 tggcggcagc tggaaagtat ggtaattatc ttggagaggt taatttaact tttgaggcac
    11461 ataaagtagt acataaaact gcaaagatta ttcctttaga aacattacct gaagttgaaa
    11521 cttcatttga agaagaagga aaaacgttaa tgtccaattc agtaattcaa catccagtag
    11581 tgcttaagcg tagtatgaat cacataactg aagctgcata cttattagct caaagtgttt
    11641 gtgagtatac acatgcacaa tgtgccatca tcaatgctgg cttactcgtt aaagatattg
    11701 taaaagatga agtgacagaa tatgacattc atcaaatgtt accgcatccg attaatatgg
    11761 taagggttag actttttggt gtgaaattaa aagagattat agctaaaagt aataaacaag
    11821 aatatatgta tgaacatgca caaggtttgg gtttcagagg gaatatattt ggaggatata
    11881 ttctttataa tttagggtac attcattcta cagggcgtta ctatctgaat ggagaagaaa
    11941 tcgaagacga caaagaatat gtactaggta cgatagatat gtatacgttc ggtcgttatt
    12001 tcccaacatt gaaagaatta ccaaaagagt atttaatgcc agagttttta agagatatat
    12061 ttaaagaaaa attattggaa tattaaaaag taagattatt ggattttcat ttgtcatgaa
    12121 tttcgatata atgtttaaag atacacttaa caggagggta tgtgttgtta tggcgacaaa
    12181 aaacgaggaa atattacgta aaccggattg gttgaaaata aaattaaata ccaacgaaaa
    12241 ctatacagga cttaagaaga tgatgaggga aaaaaatctt aatactgtat gtgaagaagc
    12301 taaatgtcct aatatacatg aatgttgggg tgcacgtcgt acagcgacat ttatgatttt
    12361 aggtgccgta tgtacaagag cttgtcgttt ttgtgcggtt aagacaggtt tacctaatga
    12421 acttgattta aatgagcctg aacgtgtagc tgaatcagtt gaattaatga atttgaaaca
    12481 cgttgttatc actgctgttg cgcgtgatga tttaagagat gctggttcaa atgtttatgc
    12541 tgagacagta cgtaaagtta gagaaagaaa tccatttaca acgattgaaa ttttaccatc
    12601 agatatgggc ggggactatg atgcgttaga aacattaatg gcgtcaagac ctgacatttt
    12661 aaaccataat attgaaactg ttcgtcgctt aacaccgaga gttcgtgcgc gtgcgactta
    12721 cgacagaaca ttagagtttt tacgtcgttc aaaagaatta caaccggata tcccaactaa
    12781 atcaagtatt atggttggat taggtgaaac tatagaagaa atttatgaaa cgatggatga
    12841 tttacgtgcg aatgatgtag atattttaac gattggtcaa tatttacaac cttcacgtaa
    12901 acatttaaag gttcaaaaat attacacgcc tttagagttt ggtaaattaa gaaaagtggc
    12961 aatggataaa gggtttaaac attgccaagc tggaccttta gtacgtagtt cttatcatgc
    13021 ggatgagcaa gtaaatgaag ctgctaaaga aaagcaacgc caaggtgagg cacagttaaa
    13081 tagttaatat ttaaccatta ataaggcata aaggcttagt ttgtacaaaa cgaacgtgtc
    13141 atagaagtaa tcattatttt tatgaaacac taaaagtata aactgagcct ttcattgtca
    13201 tagataggtg aagaatttga taaaagtaga tcaacattac tttgaattaa tagaaaatta
    13261 tcgcgaatgt tttaatgaag aacaatttat tgctaggtat tcagatattt tagataaata
    13321 tgattacata gttggtgact atggttacga tcaattacga ttaaaaggtt tttacaaaga
    13381 ttctaataaa aaagcagaga tgagtaaacg tttttcaaat attcaagatt acatatttga
    13441 atattgtaac tttggttgtc cttactttgt attaagacat ttgtctaaac aagaggttaa
    13501 aaagttaatc gaagaagttc atccgtctga tgtgatagat gacgacaata aacttcaaga
    13561 tgtgaagatt aagccaacca ttcaagatac tgaacattaa taaaaccctt agctagattg
    13621 aaaatgggaa tcatgcaatt caagcatgga cctgtaatct agttaggggt ttttatcttt
    13681 aatgaatgac ttcatttaaa tactcagtaa tttcatcgcc ttcttcagca tttacaccta
    13741 aaatatgagc gatatagcct tcttctttta aatcatcagt accgataata ccgaatttat
    13801 ttgtttgcat attaagtacg agtgtcttac cataatgtct atttgtatgg actaacatca
    13861 aatcatatcg actatgctcg ccaacaaaac caacaaactg aacttgactc tcttcgttgt
    13921 catcatataa atacatatca atcattttgt agcgactcct tttaaaagta gtaaagttag
    13981 tataacgaca aatgaagtat actgcaaaat tatgataata tataagtgag aggtgacaag
    14041 gaatgtattt tgtagacaaa gataaactaa ctcagaaatt agcctattta caagcattaa
    14101 ctgatgatta tcatgagagc aagcacaatc attatgcatt tgaacgcatt gctcaaatgt
    14161 tgatagaatc atcggtagat atagggaata tgattatcga tgcatttatt ttaagggatc
    14221 ctggtaatta taaagatgtg attgatatat tagaactaga aaatgttatt actaaagaaa
    14281 cacagcaggc gattaataaa actgtcggta ttcgtaaaca atttacatat gattacacag
    14341 ccttagatat taagattatc atgccaatgt ttgatgacgc attaccttat tacaaacaat
    14401 ttattacaga agtaacgaca tttttacatc aagaaaatgt accagtaaca gcttttggta
    14461 aaggagaaaa tcaataatga aacagtataa agcgtattta atcgatttag atggcacaat
    14521 gtatatggga acagatgaga ttgatggagc aaaacaattc atcgattatt taaatgtaaa
    14581 aggcattcct catttatacg taactaataa ttcaacaaaa acacctgagc aagtaactga
    14641 aaaattacgt gaaatgcaca ttgatgctaa accagaagag gttgtaacgt cagcgttagc
    14701 cactgctgat tatatttcag aacaatcacc aggagcatca gtatatatgt taggtgggag
    14761 tggtttaaat actgcgttaa ccgaagcggg acttgtcatt aaaaatgacg agcatgttga
    14821 ttatgtagtt attggacttg acgaacaagt tacatatgaa aagcttgcga ttgcaacgtt
    14881 aggtgtaaga aatggtgcaa catttatttc tacaaatcct gatgtatcaa ttcctaaaga
    14941 gcgtggttta ttacctggta atggtgctat tacaagtgtt gtaagtgtat cgacaggtgt
    15001 atcgccacaa tttattggta aaccagaacc gattattatg gttaaagcat tagaaatttt
    15061 aggattagat aaatccgaag ttgctatggt aggcgatttg tacgataccg atattatgtc
    15121 tggtattaac gtaggtatgg atacgattca tgtacaaaca ggtgtatcta cgttagaaga
    15181 tgtgcaaaat aaaaatgtgc caccaacgta ttcttttaaa gatttaaatg aagcaatagc
    15241 tgaattagaa aaatagatat agtcatttta taaagtaggt gaattgattt ggtaaaaata
    15301 gttgtttcga ggaaaattcc agataaattt tatcaacaat taagtaaact tggtgacgtt
    15361 gttatgtggc aaaaatcatt agtgcctatg cctaaagatc aatttgtgac agcacttcgt
    15421 gacgcagatg cttgttttat tacattaagt gaacagatcg atgcagaaat tttagcgcaa
    15481 tcaccaaatt taaaagtaat tgcgaatatg gctgtaggat atgacaacat cgatgttgaa
    15541 agtgcaacag cgaataacgt ggttgtcacg aatacaccaa atgtacttac tgaaacaact
    15601 gcagaattag gatttacatt aatgcttgct atagcacgcc gtattgtaga agctgaaaaa
    15661 tatgtagaag cagatgcatg gcaaagctgg ggtccttatt tattgtcagg taaagatgtc
    15721 ttcaattcaa ctattggaat atatggtatg ggagatattg gtaaagcttt tgcaagaagg
    15781 ttgcaagggt ttaatactaa tattctttat cataatcgat caagacataa agatgcagag
    15841 gcggacttta atgcaacata tgtttctttt gaaacgttgt tagcagaaag tgattttatc
    15901 atctgtacag cgccacttac aaaagaaaca catcataaat ttaatgctga agcatttgaa
    15961 caaatgaaaa atgatgcaat ttttattaat atcggtagag gacaaattgt agatgaaaca
    16021 gcattaatcg atgcactaga caataaagaa attttagcat gtggtttaga tgtattagca
    16081 aatgaaccga ttgatcatac acatccatta atgggacgtg ataatgttct gattacacca
    16141 cacattggta gcgcatcagt aacaacacgg gacaatatga ttcaattatg tattaataat
    16201 atagaagcgg ttatgacaaa tcaggtacca catactccag taaattgaaa ataagtatgg
    16261 tttttaatca ttggtaaaac aagcaaagca attgttaaat atgttatgct tgacttgcga
    16321 atgtatatat ttgcgctgat ga
//
LOCUS       DAKTIO010000048        16067 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.048, whole genome shotgun sequence.
ACCESSION   DAKTIO010000048 DAKTIO010000000
VERSION     DAKTIO010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16067
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.048"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     misc_feature    52..198
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            236..736
                     /locus_tag="O0293_001159"
     CDS             236..736
                     /locus_tag="O0293_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCY0280051.1"
                     /translation="MSAIIEAKKQLVDEIAEVLSNSVSTVIVDYRGLTVAEVTDLRSQ
                     LREAGVEYKVYKNTMVRRAAEKAGIEGLDEFLTGPTAIATSSEDAVAAAKVISGFAKD
                     HEALEIKSGVMEGNVITAEEVKTVGSLPSHDGLVSMLLSVLQAPVRNFAYAVKAIGEQ
                     KEENAE"
     gene            779..1147
                     /gene="rplL"
                     /locus_tag="O0293_001160"
     CDS             779..1147
                     /gene="rplL"
                     /locus_tag="O0293_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCY0280052.1"
                     /translation="MANHEQIIEAIKEMSVLELNDLVKAIEEEFGVTAAAPVAVAGAA
                     GGADAAAEKTEFDVELTSAGSSKIKVVKAVKEATGLGLKDAKELVDGAPKVIKEALPK
                     EEAEKLKEQLEEVGATVELK"
     gene            1322..1930
                     /locus_tag="O0293_001161"
     CDS             1322..1930
                     /locus_tag="O0293_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0280053.1"
                     /translation="MSHYYDEDPSVISNEQRIQYQLNHHKIDLITDNGVFSKDKVDYG
                     SDVLVQTFLKAHPPGPSKRIADVGCGYGPIGLMIAKVSPHHSITMLDVNHRALALVEK
                     NKKLNGIDNVIVKESDALSAVEDKSFDFILTNPPIRAGKETVHRIFEQALHRLDSNGE
                     LFVVIQKKQGMPSAKKRMNELFGNVEVVNKDKGYYILRSIKA"
     gene            2145..5696
                     /gene="rpoB"
                     /locus_tag="O0293_001162"
     CDS             2145..5696
                     /gene="rpoB"
                     /locus_tag="O0293_001162"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCY0280054.1"
                     /translation="MAGQVVQYGRHRKRRNYARISEVLELPNLIEIQTKSYEWFLREG
                     LIEMFRDISPIEDFTGNLSLEFVDYRLGEPKYDLEESKNRDATYAAPLRVKVRLIIKE
                     TGEVKEQEVFMGDFPLMTDTGTFVINGAERVIVSQLVRSPSVYFNEKIDKNGRENYDA
                     TIIPNRGAWLEYETDAKDVVYVRIDRTRKLPLTVLLRALGFSSDQEIVDLLGDNEYLR
                     NTLEKDGTENTEQALLEIYERLRPGEPPTVENAKSLLYSRFFDPKRYDLASVGRYKTN
                     KKLHLKHRLFNQKLAEPIVNTETGEIVVEEGTVLDRRKIDEIMDVLESNANSEVFELH
                     GSVIDEPVEIQSIKVYVPNDDEGRTTTVIGNAFPDSEVKCITPADIIASMSYFFNLLS
                     GIGYTDDIDHLGNRRLRSVGELLQNQFRIGLSRMERVVRERMSIQDTESITPQQLINI
                     RPVIASIKEFFGSSQLSQFMDQANPLAELTHKRRLSALGPGGLTRERAQMEVRDVHYS
                     HYGRMCPIETPEGPNIGLINSLSSYARVNEFGFIETPYRKVDLDTHAITDQIDYLTAD
                     EEDSYVVAQANSKLDENGRFMDDEVVCRFRGNNTVMAKEKMDYMDVSPKQVVSAATAC
                     IPFLENDDSNRALMGANMQRQAVPLMNPEAPFVGTGMEHVAARDSGAAITAKHRGRVE
                     HVESNEILVRRLVEENGVEHEGELDRYPLAKFKRSNSGTCYNQRPIVAVGDVVEYNEI
                     LADGPSMELGEMALGRNVVVGFMTWDGYNYEDAVIMSERLVKDDVYTSIHIEEYESEA
                     RDTKLGPEEITRDIPNVSESALKNLDDRGIVYIGAEVKDGDILVGKVTPKGVTELTAE
                     ERLLHAIFGEKAREVRDTSLRVPHGAGGIVLDVKVFNREEGDDTLSPGVNQLVRVYIV
                     QKRKIHVGDKMCGRHGNKGVISKIVPEEDMPYLPDGRPIDIMLNPLGVPSRMNIGQVL
                     ELHLGMAAKNLGIHVASPVFDGANDDDVWSTIEEAGMARDGKTVLYDGRTGEPFDNRI
                     SVGVMYMLKLAHMVDDKLHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGA
                     AYTLQEILTYKSDDTVGRVKTYEAIVKGENISRPSVPESFRVLMKELQSLGLDVKVMD
                     EQDNEIEMTDVDDDDVVERKVDLQQNDAPETQKEVTD"
     gene            5860..9456
                     /gene="rpoC"
                     /locus_tag="O0293_001163"
     CDS             5860..9456
                     /gene="rpoC"
                     /locus_tag="O0293_001163"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCY0280055.1"
                     /translation="MKIGLASPEKIRSWSFGEVKKPETINYRTLKPEKDGLFCERIFG
                     PTKDWECSCGKYKRVRYKGMVCDRCGVEVTKSKVRRERMGHIELAAPVSHIWYFKGIP
                     SRMGLLLDMSPRALEEVIYFASYVVVDPGPTGLEKKTLLSEAEFRDYYDKYPGQFVAK
                     MGAEGIKDLLEEIDLDEELKLLRDELESATGQRLTRAIKRLEVVESFRNSGNKPSWMI
                     LDVLPIIPPEIRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPGIIVQNEK
                     RMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGKRVDYSGRSV
                     IAVGPSLKMYQCGLPKEMALELFKPFVMKELVQREIATNIKNAKSKIERMDDEVWDVL
                     EEVIREHPVLLNRAPTLHRLGIQAFEPTLVEGRAIRLHPLVTTAYNADFDGDQMAVHV
                     PLSKEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTLERKDAVNTGAIFN
                     NTNEVLKAYANGFVHLHTRIGVHASSFNNPTFTEEQNKKILATSVGKIIFNEIIPDSF
                     AYINEPTQENLERKTPNRYFIDPTTLGEGGLKEYFENEELIEPFNKKFLGNIIAEVFN
                     RFSITDTSMMLDRMKDLGFKFSSKAGITVGVADIVVLPDKQQILDEHEKLVDRITKQF
                     NRGLITEEERYNAVVEIWTDAKDQIQGELMQSLDKTNPIFMMSDSGARGNASNFTQLA
                     GMRGLMAAPSGKIIELPITSSFREGLTVLEYFISTHGARKGLADTALKTADSGYLTRR
                     LVDVAQDVIVREEDCGTDRGLLVSDIKEGTEMIEPFIERIEGRYSKETIHHPETDEII
                     IRPDELITPEIAKKITDAGIEQMYIRSAFTCNARHGVCEKCYGKNLATGEKVEVGEAV
                     GTIAAQSIGEPGTQLTMRTFHTGGVAGSDITQGLPRIQEIFEARNPKGQAVITEIEGV
                     VEDIKLAKDRQQEIVVKGANETRSYLASGTSRIIVEIGQPVQRGEVLTEGSIEPKNYL
                     SVAGLNATESYLLKEVQKVYRMQGVEIDDKHVEVMVRQMLRKVRIIEAGDTKLLPGSL
                     VDIHNFTDANREAFKHRKRPATAKPVLLGITKASLETESFLSAASFQETTRVLTDAAI
                     KGKRDDLLGLKENVIIGKLIPAGTGMRRYSDVKYEKTAKPVAEVESQTEVTE"
     gene            9593..9847
                     /locus_tag="O0293_001164"
     CDS             9593..9847
                     /locus_tag="O0293_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HCY0280056.1"
                     /translation="MSKEKVARFNKQHFVVGLKETLKALKKDQVTSLIIAEDVEVYLM
                     TRVLSQINQKNIPVSFFKSKHALGKHVGINVNATIVALIK"
     gene            9945..10358
                     /gene="rpsL"
                     /locus_tag="O0293_001165"
     CDS             9945..10358
                     /gene="rpsL"
                     /locus_tag="O0293_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCY0280057.1"
                     /translation="MPTINQLVRKPRQSKIKKSDSPALNKGFNSKKKKFTDLNSPQKR
                     GVCTRVGTMTPKKPNSALRKYARVRLSNNIEINAYIPGIGHNLQEHSVVLVRGGRVKD
                     LPGVRYHIVRGALDTSGVDGRRQGRSLYGTKKPKN"
     gene            10424..10894
                     /gene="rpsG"
                     /locus_tag="O0293_001166"
     CDS             10424..10894
                     /gene="rpsG"
                     /locus_tag="O0293_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCY0280058.1"
                     /translation="MPRKGSVPKRDVLPDPIHNSKLVTKLINKIMLDGKRGTAQRILY
                     SAFDLVEQRSGRDALEVFEEAINNIMPVLEVKARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNYARLRGEKTMEDRLANEILDAANNTGGAVKKREDTHKMAEANKAFAHYRW"
     gene            11017..13098
                     /gene="fusA"
                     /locus_tag="O0293_001167"
     CDS             11017..13098
                     /gene="fusA"
                     /locus_tag="O0293_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCY0280059.1"
                     /translation="MAREFSLEKTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETH
                     EGASQMDWMEQEQDRGITITSAATTAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFVNKMDKLGANFEYSVSTLHDRLQANAA
                     PIQLPIGAEDEFEAIIDLVEMKCFKYTNDLGTEIEEIEIPEDHLDRAEEARASLIEAV
                     AETSDELMEKYLGDEEISVSELKEAIRQATTNVEFYPVLCGTAFKNKGVQLMLDAVID
                     YLPSPLDVKPIIGHRASNPEEEVIAKADDSAEFAALAFKVMTDPYVGKLTFFRVYSGT
                     MTSGSYVKNSTKGKRERVGRLLQMHANSRQEIDTVYSGDIAAAVGLKDTGTGDTLCGE
                     KNDIILESMEFPEPVIHLSVEPKSKADQDKMTQALVKLQEEDPTFHAHTDEETGQVII
                     GGMGELHLDILVDRMKKEFNVECNVGAPMVSYRETFKSSAQVQGKFSRQSGGRGQYGD
                     VHIEFTPNETGAGFEFENAIVGGVVPREYIPSVEAGLKDAMENGVLAGYPLIDVKAKL
                     YDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVILEPMMKVTIEMPEEYMGDIMGDVT
                     SRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRSNTQGRGTYTMYFDHYAEVPKSI
                     AEDIIKKNKGE"
     gene            13315..14499
                     /gene="tuf"
                     /locus_tag="O0293_001168"
     CDS             13315..14499
                     /gene="tuf"
                     /locus_tag="O0293_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCY0280060.1"
                     /translation="MAKEKFDRSKEHANIGTIGHVDHGKTTLTAAIATVLAKNGDSVA
                     QSYDMIDNAPEEKERGITINTSHIEYQTDKRHYAHVDCPGHADYVKNMITGAAQMDGG
                     ILVVSAADGPMPQTREHILLSRNVGVPALVVFLNKVDMVDDEELLELVEMEVRDLLSE
                     YDFPGDDVPVIAGSALKALEGDAQYEEKILELMEAVDTYIPTPERDSDKPFMMPVEDV
                     FSITGRGTVATGRVERGQIKVGEEVEIIGLHDTSKTTVTGVEMFRKLLDYAEAGDNIG
                     ALLRGVAREDVQRGQVLAAPGSITPHTEFKAEVYVLSKDEGGRHTPFFSNYRPQFYFR
                     TTDVTGVVHLPEGTEMVMPGDNVEMTVELIAPIAIEDGTRFSIREGGRTVGSGVVTEI
                     IK"
     gene            complement(14781..15956)
                     /locus_tag="O0293_001169"
     CDS             complement(14781..15956)
                     /locus_tag="O0293_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCY0280061.1"
                     /translation="MLDWFQLANNKENKTIQLRRYLHQYPELSFEEFQTHDYIVNQLS
                     QLSCDIETPIGRNGIKATFKGLGTGPTIALRADFDALPVEELNDVPYKSKNPGCMHAC
                     GHDGHTAILLTVAEILDEHKHLLEGNVVLIFQYGEEIMPGGSQEMIDAGCLENVDRIY
                     GTHLWSGYPTGTIHSRAGAIMASPDEFSVTFKGRGGHGAKPHETIDPIVIMAEFILSA
                     QKIISRTIDPVKQAVLSFGMIQAGTTDSVIPDQAFCKGTVRTFDSDIQNHVMDKMDKL
                     LQGLAIANDINYDLNYIKGYLPVHNNEKAYQVIKEATNDLHVRFNESDLMMIGEDFSH
                     YLKVRPGAFFLTGCGNESKGITAPHHNPKFDIDEKSLKYAVAVFLKIIELEQVFKTN"
BASE COUNT         5474 a         2633 c         3158 g         4802 t
ORIGIN      
        1 ctttcagtct gtaaaaatat attgacaata agtaatttcc aagttatatt acttattgtg
       61 attattttac ctaagacagt aggagttatt tataacttaa aatttatcct gccgaggcta
      121 aaattgactt gaacgtgatg atctatgatc tttcaagcac tttttgccgt gggtagaaag
      181 tgcttttttt attaatttta aaaaaagcac caaaaattta aatggaggtg tctgaatgtc
      241 tgctatcatt gaagctaaaa aacaactagt tgatgaaatt gctgaggtac tatcaaattc
      301 agtttcaaca gtaatcgttg actaccgtgg attaacagta gctgaagtta ctgacttacg
      361 ttcacaatta cgtgaagctg gtgttgagta taaagtatac aaaaacacta tggtacgtcg
      421 tgcagctgaa aaagctggta tcgaaggctt agatgaattc ttaacaggtc ctactgctat
      481 tgcaacttca agtgaagatg ctgtagctgc agcgaaagta atttctggat ttgctaaaga
      541 tcatgaagca ttagaaatta aatcaggcgt tatggaaggc aatgttatta cagcagaaga
      601 agttaaaact gttggttcat taccttcaca cgatggtctt gtatctatgc ttttatcagt
      661 attacaagct cctgtacgca acttcgctta tgcggttaaa gctattggag aacaaaaaga
      721 agaaaacgct gaataatttt tagcgtaaaa aaattaaaaa taatggagga attataaaat
      781 ggctaatcat gaacaaatca ttgaagcgat taaagaaatg tcagtattag aattaaacga
      841 cttagtaaaa gcaattgaag aagaatttgg tgtaactgca gctgctccag tagcagtagc
      901 aggtgcagct ggtggcgctg acgctgcagc agaaaaaact gaatttgacg ttgagttaac
      961 ttcagctggt tcatctaaaa tcaaagttgt taaagctgtt aaagaagcaa ctggtttagg
     1021 attaaaagat gctaaagaat tagtagacgg agctcctaaa gtaatcaaag aagctttacc
     1081 taaagaagaa gctgaaaaac ttaaagaaca attagaagaa gttggagcta ctgtagaatt
     1141 aaaataattc aagtatctta aacttaataa tcaaagtttt atagcaagta ttgctataat
     1201 ataatgattc tttgagaagt taaaaccccg ttattttgat aacggggttt tattcattta
     1261 aagactgagt gaaatgttat aattataatg acgagttaca aagtgaagat gaggtggaat
     1321 aatgagtcat tattacgatg aagatccaag tgtaattagc aatgaacaac gtattcaata
     1381 tcaattaaac catcataaaa ttgatttaat aactgataac ggagtgtttt cgaaagataa
     1441 agtagattat ggttcagatg ttcttgttca aactttttta aaagcgcatc cacctggtcc
     1501 aagtaagcga attgccgatg ttggttgtgg ttacggacca attggtttga tgattgctaa
     1561 agtatcacca catcattcaa ttacaatgct agatgttaat cacagagcgc tagccttagt
     1621 tgaaaaaaac aaaaaattaa atggtattga taatgtgatc gtaaaggaaa gtgatgcttt
     1681 gtctgctgtg gaagacaaaa gttttgattt tattttaacc aatccaccaa taagagcagg
     1741 gaaagaaacc gtgcatcgta tattcgagca agcattacat agattagact cgaacggtga
     1801 actattcgtt gtaattcaga agaagcaagg tatgccatct gcaaagaaaa gaatgaatga
     1861 actttttgga aatgtagaag tggtaaataa agataaagga tattacattc tgagaagtat
     1921 aaaagcttga aatgaaatgg atattctgtt atagttatat aatgtaaaaa tttatgttca
     1981 ataagtgtgt acttttacgt taaatagata agttaattaa gaataaatat agaatcgaaa
     2041 atggtgtcat cattagtgtt gccgttttct ttttgtcttt ttattaatat gcttatggta
     2101 tttagctaaa agcggatcac ataatttttg aggggtgaat ctgtttggca ggtcaagttg
     2161 tccaatatgg aagacatcgt aaacgtagaa actacgcgag aatttcagaa gtattagaat
     2221 taccaaactt aatagaaatt caaactaaat cttacgagtg gttcctaaga gaaggtttaa
     2281 tcgaaatgtt tagagacatt tctccaattg aagattttac tggtaatttg tcattagagt
     2341 ttgtggatta ccgtttagga gaaccaaaat atgatttaga agaatctaaa aaccgtgacg
     2401 ctacttatgc tgcacctctt cgtgtaaaag tgcgtctaat cattaaagaa acaggagaag
     2461 ttaaagaaca agaagtcttt atgggtgatt tcccattaat gactgataca ggtacgttcg
     2521 ttatcaatgg tgcagaacgt gtaatcgtat ctcaattagt tcgttcacca tccgtttatt
     2581 tcaatgaaaa aatcgacaaa aatggtcgtg aaaactatga tgcaacaatt attccaaacc
     2641 gtggtgcatg gttagaatat gaaacagatg ctaaagatgt tgtatacgta cgtattgata
     2701 gaacacgtaa actaccatta acagtattgt tacgtgcatt aggtttctca agcgaccaag
     2761 aaattgttga ccttttaggt gacaatgaat atttacgtaa tactttagag aaagacggca
     2821 ctgaaaacac tgaacaagcg ttattagaaa tctatgaacg tttacgtcca ggtgaaccac
     2881 caactgttga aaatgctaaa agtctattgt attcacgttt ctttgatcca aaacgctatg
     2941 acttagcaag cgtgggtcgt tataaaacaa acaaaaaatt acatttaaaa catcgtttat
     3001 ttaatcaaaa attagctgag ccaattgtaa atactgaaac tggtgaaatt gtagttgaag
     3061 aaggtacagt gcttgatcgt cgtaaaatcg acgaaatcat ggatgtactt gaatcaaatg
     3121 caaacagcga agtgtttgaa ttgcatggta gcgttataga cgagccagta gaaattcaat
     3181 caattaaagt atatgttcct aacgatgatg aaggtcgtac gacaactgta attggtaatg
     3241 ctttccctga ctcagaagtt aaatgcatta caccagcaga tatcattgct tcaatgagtt
     3301 acttctttaa cttattaagc ggtattggat atacagatga tattgaccat ttaggtaacc
     3361 gtcgtttacg ttctgtaggt gaattactac aaaaccaatt ccgtatcggt ttatcaagaa
     3421 tggaaagagt tgtacgtgaa agaatgtcaa ttcaagatac tgagtctatc acacctcaac
     3481 aattaattaa tattcgacct gttattgcat ctattaaaga attctttggt agctctcaat
     3541 tatcacaatt catggaccaa gcaaacccat tagctgagtt aacgcataaa cgtcgtctat
     3601 cagcattagg acctggtggt ttaacacgtg aacgtgctca aatggaagta cgtgacgttc
     3661 actactctca ctatggccgt atgtgtccaa ttgaaacacc tgagggacca aacattggat
     3721 tgattaactc attatcaagt tatgcacgtg taaatgaatt cggctttatt gaaacaccat
     3781 atcgtaaagt tgatttagat acacatgcta tcactgatca aattgactat ttaacagctg
     3841 acgaagaaga tagctatgtt gtagcacaag caaactctaa attagatgaa aatggtcgtt
     3901 tcatggatga tgaagttgta tgtcgtttcc gtggtaacaa tacagttatg gctaaagaaa
     3961 aaatggatta tatggatgta tcgccgaagc aagttgtttc agcagcgaca gcatgtattc
     4021 cattcttaga aaatgatgac tcaaaccgtg cattgatggg tgcgaacatg caacgtcaag
     4081 cagtgccttt gatgaatcca gaagcaccat ttgttggtac aggtatggaa cacgttgcag
     4141 cacgtgattc tggtgcggct attacagcta agcacagagg tcgtgttgaa catgttgaat
     4201 ctaatgaaat tcttgttcgt cgtctagttg aagagaacgg cgttgagcat gaaggtgaat
     4261 tagatcgcta tccattagct aaatttaaac gttcaaactc aggtacatgt tacaaccaac
     4321 gtccaatcgt tgcagttgga gatgttgttg agtataacga gattttagca gatggaccat
     4381 ctatggaatt aggagaaatg gcattaggta gaaacgtagt agttggtttc atgacttggg
     4441 acggttacaa ctatgaggat gccgttatca tgagtgaaag acttgtgaaa gatgacgtgt
     4501 atacttctat tcatattgaa gagtatgaat cagaagcacg tgatactaag ttaggacctg
     4561 aagaaatcac aagagatatt cctaatgttt ctgaaagtgc acttaagaac ttagacgatc
     4621 gtggtatcgt ttatattggt gcagaagtaa aagatggaga tattttagtt ggtaaagtaa
     4681 cgcctaaagg tgtaactgag ttaactgccg aagaaagatt gttacatgca atctttggtg
     4741 aaaaagcacg tgaagttaga gatacttcat tacgtgtacc tcacggcgct ggcggtatcg
     4801 ttcttgatgt aaaagtattc aatcgtgaag aaggcgacga tacattatca cctggtgtaa
     4861 accaattagt acgtgtatat atcgttcaaa aacgtaaaat tcatgttggt gataagatgt
     4921 gtggtcgaca tggtaacaaa ggtgtcattt ctaagattgt tcctgaagaa gatatgcctt
     4981 acttaccaga tggacgtccg atcgatatca tgttaaatcc tcttggtgta ccatctcgta
     5041 tgaacatcgg acaagtatta gagctacact taggtatggc tgctaaaaat cttggtattc
     5101 acgttgcatc accagtattt gacggtgcaa acgatgacga tgtatggtca acaattgaag
     5161 aagctggtat ggctcgtgat ggtaaaactg tactttatga tggacgtaca ggtgaaccat
     5221 tcgataaccg tatttcagta ggtgtaatgt acatgttgaa acttgcgcac atggttgatg
     5281 ataaattaca tgcgcgttca acaggaccat attcacttgt tacacaacaa ccacttggcg
     5341 gtaaagcgca attcggtgga caacgttttg gtgagatgga ggtatgggca cttgaagcat
     5401 atggtgctgc atacacatta caagaaatct taacttacaa atccgatgat acagtaggac
     5461 gtgtgaaaac atacgaggct attgttaaag gtgaaaacat ctctagacca agtgttccag
     5521 aatcattccg agtattgatg aaagaattac aaagtttagg tttagatgta aaagttatgg
     5581 atgagcaaga taatgaaatc gaaatgacag acgttgatga cgatgatgtt gtagaacgca
     5641 aagtagattt acaacaaaat gatgctcctg aaacacaaaa agaagttact gattaatacg
     5701 caatttacaa aacaggcaaa aagatactaa gctgaatttt attgatgatt cagtttagta
     5761 ctttaagcca ttttaaataa atgcaaatca atcaaatagc acagctaatc taaattgaag
     5821 gaggtaggct ccttgattga tgtaaataat ttccattata tgaaaatagg attggcttca
     5881 cctgaaaaaa tccgttcttg gtcttttggt gaagttaaaa aacctgaaac aatcaactac
     5941 cgtacattaa aacctgaaaa agatggtcta ttctgtgaaa gaattttcgg acctacaaaa
     6001 gactgggaat gtagttgtgg taaatacaaa cgtgttcgct acaaaggcat ggtctgtgac
     6061 agatgtggag ttgaagtaac taaatctaaa gtacgtcgtg aaagaatggg tcacattgaa
     6121 cttgctgctc cagtttctca catttggtat ttcaaaggta taccaagtcg tatgggatta
     6181 ttacttgaca tgtcaccaag agcattagaa gaagttattt actttgcttc ttatgttgtt
     6241 gtagatccag gtccaactgg tttagaaaag aaaactttat tatctgaagc tgaattcaga
     6301 gattattatg ataaataccc aggtcaattc gttgcaaaaa tgggtgcaga aggtattaaa
     6361 gatttacttg aagagattga tcttgacgaa gaacttaaat tgttacgcga tgagttggaa
     6421 tcagctactg gtcaaagact tactcgtgca attaaacgtt tagaagttgt tgaatcattc
     6481 cgtaattcag gtaacaaacc ttcatggatg attttagatg tacttccaat catcccacca
     6541 gaaattcgtc caatggttca attagatggt ggacgatttg caacaagtga cttaaacgac
     6601 ttataccgtc gtgtaattaa tcgaaataat cgtttgaaac gtttattaga tttaggtgca
     6661 cctggtatca tcgttcaaaa cgaaaaacgt atgttacaag aagccgttga cgctttaatt
     6721 gataatggtc gtcgtggtcg tccagttact ggcccaggta accgtccatt aaaatcttta
     6781 tctcatatgt taaaaggtaa acaaggtcgt ttccgtcaaa acttacttgg taaacgtgtt
     6841 gactattcag gacgttcagt tattgcagta ggtccaagct tgaaaatgta ccaatgtggt
     6901 ttaccaaaag aaatggcact tgaactattt aaaccattcg taatgaaaga attagttcaa
     6961 cgtgaaattg caactaacat taaaaatgcg aagagtaaaa tcgaacgtat ggatgatgaa
     7021 gtttgggacg tattggaaga agtaattaga gaacatcctg tattacttaa ccgtgcacca
     7081 acacttcata gacttggtat tcaagcattt gaaccaactt tagttgaagg tcgtgcgatt
     7141 cgtctacatc cacttgtaac aacagcttat aacgctgact ttgacggtga ccaaatggcg
     7201 gttcacgttc ctttatcaaa agaggcacaa gctgaagcaa gaatgttgat gttagcagca
     7261 caaaacatct tgaaccctaa agatggtaaa cctgtagtta caccatcaca agatatggta
     7321 cttggtaact attaccttac tttagaaaga aaagatgcag taaatacagg cgcaatcttt
     7381 aataatacaa atgaagtatt aaaagcatat gcaaatggct ttgtacattt acacactaga
     7441 attggtgtac atgcaagttc gttcaataat ccaacattta ctgaagaaca aaacaaaaag
     7501 attcttgcta cgtcagtagg taaaattata ttcaatgaaa tcattccaga ttcatttgct
     7561 tatattaatg aacctacgca agaaaactta gaaagaaaga caccaaacag atatttcatc
     7621 gatcctacaa ctttaggtga aggtggatta aaagaatact ttgaaaatga agaattaatt
     7681 gaacctttca acaaaaaatt cttaggtaat attattgcag aagtattcaa cagatttagc
     7741 atcactgata catcaatgat gttagaccgt atgaaagact taggattcaa attctcatct
     7801 aaagctggta ttacagtagg tgttgctgat atcgtagtat tacctgataa gcaacaaata
     7861 cttgatgagc atgaaaaatt agtcgacaga attacaaaac aattcaaccg tggtttaatc
     7921 actgaagaag aaagatataa tgcagttgtt gaaatttgga cagatgcaaa agatcaaatt
     7981 caaggtgaat tgatgcaatc acttgataaa actaacccaa tcttcatgat gagtgattca
     8041 ggtgcccgtg gtaacgcatc taactttaca cagttagcag gtatgcgtgg attgatggcc
     8101 gcaccatctg gtaagattat cgaattacca atcacatctt cattccgtga aggtttaaca
     8161 gtacttgaat acttcatctc aactcacggt gcacgtaaag gtcttgccga tacagcactt
     8221 aaaacagctg actcaggata tcttactcgt cgtcttgttg acgtggcaca agatgttatt
     8281 gttcgtgaag aagactgtgg tactgataga ggtttattag tttctgatat taaagaaggt
     8341 acagaaatga ttgaaccatt tatcgaacgt attgaaggtc gttattctaa agaaacaatt
     8401 catcatcctg aaactgatga aataatcatt cgtcctgatg aattaattac acctgaaatt
     8461 gctaagaaaa ttacagatgc tggtattgaa caaatgtata ttcgctcagc atttacttgt
     8521 aacgcacgac atggtgtttg tgaaaaatgt tacggtaaaa accttgctac tggtgaaaaa
     8581 gttgaagttg gtgaagcagt tggtacaatt gcagcccaat ctatcggtga accaggtaca
     8641 cagcttacaa tgcgtacatt ccatacaggt ggggtagcag gtagcgatat cacacaaggt
     8701 cttcctcgta ttcaagagat tttcgaagca cgtaacccta aaggtcaagc ggtaattacg
     8761 gaaatcgaag gtgtcgtaga agatattaaa ttagcaaaag atagacaaca agaaattgtt
     8821 gttaaaggtg ctaatgaaac aagatcatac cttgcttcag gtacttcaag aattattgta
     8881 gaaatcggtc aaccagttca acgtggtgaa gtattaactg aaggttctat tgaacctaag
     8941 aattacttat ctgttgctgg attaaacgcg actgaaagct acttattaaa agaagtacaa
     9001 aaagtttacc gtatgcaagg tgtagaaatc gacgataaac acgttgaggt tatggttcga
     9061 caaatgttac gtaaagttag aattatcgaa gcaggtgata cgaagttatt accaggttca
     9121 ttagttgata ttcataactt tacagatgca aatagagaag catttaaaca ccgtaagcgt
     9181 cctgcaacag ctaaaccagt attacttggt attactaaag catcacttga aacagaaagt
     9241 ttcttatctg cagcatcatt ccaagaaaca acaagagttc ttacagatgc agcaattaaa
     9301 ggtaagcgtg atgacttatt aggtcttaaa gaaaacgtaa ttattggtaa gttaattcca
     9361 gctggtactg gtatgagacg ttatagcgac gtaaaatacg aaaaaacagc taaaccagtt
     9421 gcagaagttg aatctcaaac tgaagtaacg gaataacaag tatataacag aggctaatgc
     9481 tttagcctct tgttattttt atgtaaatta tttgatttaa tgttgacgaa ttctcttgtt
     9541 caatgttaat atattaaagg ttgatgcaag cagaactttg gaggataaat tattgtctaa
     9601 ggaaaaagtt gcacgcttta acaaacaaca ttttgtagtt ggtcttaaag aaacgcttaa
     9661 agcgttaaag aaagatcaag ttacatcttt gattattgct gaagacgttg aagtatattt
     9721 aatgactcgc gtgttaagcc aaatcaatca gaaaaatata cctgtatctt ttttcaaaag
     9781 caaacatgct ttgggtaaac atgtaggtat taacgtcaat gcgacaatag tagcattgat
     9841 taaatgagaa ttagtaagtg ttttacttac taaattttat ttaacctaaa aatgaaccac
     9901 ctggatgtgt gggattaaaa agtgaagaga ggaggacata tcacatgcca actattaacc
     9961 aattagtacg taaaccaaga caaagcaaaa tcaaaaaatc agattctcca gctttaaata
    10021 aaggtttcaa cagtaaaaag aaaaaattta ctgacttaaa ctcaccacaa aaacgtggtg
    10081 tatgtactcg tgtaggtaca atgacaccta aaaaacctaa ctcagcgtta cgtaaatatg
    10141 cacgtgtgcg tttatcaaac aacatcgaaa ttaacgcata catccctggt atcggacata
    10201 acttacaaga acacagtgtt gtacttgtac gtggtggacg tgtaaaagac ttaccaggtg
    10261 tgcgttacca tattgtacgt ggagcacttg atacttcagg tgttgacgga cgtagacaag
    10321 gtcgttcatt atacggaact aagaaaccta aaaactaaga atttagtttt taattaaatc
    10381 ttaaacttaa aatatttaat ataaggaagg gaggatttac attatgcctc gtaaaggatc
    10441 agtacctaaa agagacgtat taccagatcc aattcataac tctaagttag taactaaatt
    10501 aattaacaaa attatgttag atggtaaacg tggaacagca caaagaattc tttattcagc
    10561 attcgaccta gttgaacaac gcagtggtcg tgatgcatta gaagtattcg aagaagcaat
    10621 caacaacatt atgccagtat tagaagttaa agctcgtcgc gtaggtggtt ctaactatca
    10681 agtaccagta gaagttcgtc cagagcgtcg tactacttta ggtttacgtt ggttagttaa
    10741 ctatgcacgt cttcgtggtg aaaaaacgat ggaagatcgt ttagctaacg aaattttaga
    10801 tgcagcaaat aatacaggtg gtgccgttaa gaaacgtgag gacactcaca aaatggctga
    10861 agcaaacaaa gcatttgctc actaccgttg gtaagataaa agcttttacc ctgagtgtgt
    10921 tctatattaa tgaattttca ttaagcgttc atgcttaggg catcgccata tctatcgtat
    10981 ttattcagta atataaactg gaaggagaaa aaatacatgg ctagagaatt ttcattagaa
    11041 aaaactcgta atatcggtat catggctcac attgatgctg gtaaaacgac tacgactgaa
    11101 cgtattcttt attacactgg ccgtatccac aaaattggtg aaacacacga aggtgcttca
    11161 caaatggact ggatggagca agaacaagac cgtggtatta ctatcacatc tgctgcaaca
    11221 acagcagctt gggaaggtca ccgtgtaaac attatcgata cacctggaca cgtagacttc
    11281 actgtagaag ttgaacgttc attacgtgta cttgacggag cagttacagt acttgatgca
    11341 caatcaggtg ttgaacctca aactgaaaca gtttggcgtc aggctacaac ttatggtgtt
    11401 ccacgtatcg tatttgtaaa caaaatggac aaattaggtg ctaacttcga atactctgta
    11461 agtacattac atgatcgttt acaagctaac gctgctccaa tccaattacc aattggtgcg
    11521 gaagacgaat tcgaagcaat cattgactta gttgaaatga aatgtttcaa atatacaaat
    11581 gatttaggta ctgaaattga agaaattgaa attcccgaag accacttaga tagagctgaa
    11641 gaagctcgtg ctagcttaat cgaagcagtt gcagaaacta gcgacgaatt aatggaaaaa
    11701 tatcttggtg acgaagaaat ttcagtttct gaattaaaag aagctatccg ccaagctact
    11761 actaacgtag aattctaccc agtactttgt ggtacagctt tcaaaaacaa aggtgttcaa
    11821 ttaatgcttg acgctgtaat tgattactta ccttcaccac tagacgttaa accaattatt
    11881 ggtcaccgtg ctagcaaccc tgaagaagaa gtaatcgcga aagcagacga ttcagctgaa
    11941 ttcgctgcat tagcgttcaa agttatgact gacccttatg ttggtaaatt aacattcttc
    12001 cgtgtgtatt caggtacaat gacatctggt tcatacgtta agaactctac taaaggtaaa
    12061 cgtgaacgtg taggtcgttt attacaaatg cacgctaact cacgtcaaga aatcgatact
    12121 gtatactctg gagatatcgc tgctgcggta ggtcttaaag atacaggtac tggtgatact
    12181 ttatgtggtg agaaaaatga cattatcttg gaatcaatgg aattcccaga gccagttatt
    12241 cacttatcag tagagccaaa atctaaagct gaccaagata aaatgactca agctttagtt
    12301 aaattacaag aagaagaccc aacattccat gcacacactg acgaagaaac tggacaagtt
    12361 atcatcggtg gtatgggtga gcttcactta gacatcttag tagaccgtat gaagaaagaa
    12421 ttcaacgttg aatgtaacgt aggtgctcca atggtttcat atcgtgaaac attcaaatca
    12481 tctgcacaag ttcaaggtaa attctctcgt caatctggtg gtcgtggtca atacggtgat
    12541 gttcacattg aattcacacc aaacgaaaca ggcgcaggtt tcgaattcga aaacgctatc
    12601 gttggtggtg tagttcctcg tgaatacatt ccatcagtag aagctggtct taaagatgct
    12661 atggaaaatg gtgttttagc aggttatcct ttaattgatg ttaaagctaa attatatgat
    12721 ggttcatacc atgatgtcga ttcatctgaa atggccttca aaattgctgc atcattagca
    12781 cttaaagaag ctgctaaaaa atgtgatcct gtaatcttag aaccaatgat gaaagtaact
    12841 attgaaatgc ctgaagagta catgggtgat atcatgggtg acgtaacatc tcgtcgtgga
    12901 cgtgttgatg gtatggaacc tcgtggtaat gcacaagttg ttaatgctta tgtaccactt
    12961 tcagaaatgt tcggttatgc aacatcatta cgttcaaaca ctcaaggtcg cggtacttac
    13021 actatgtact tcgatcacta tgctgaagtt ccaaaatcaa tcgctgaaga tattatcaag
    13081 aaaaataaag gtgaataata taacttgttt tgactagcta gcctaggtta aaatacaagg
    13141 tgagcttaaa tgtaagctat catctttata gtttgatttt ttggggtgaa tgcattataa
    13201 aagaattgta aaattctttt tgcatcgcta taaataattt ctcatgatgg tgagaaacta
    13261 tcatgagaga taaatttaaa tattattttt aattagaata ggagagattt tataatggca
    13321 aaagaaaaat tcgatcgttc taaagaacat gccaatatcg gtactatcgg tcacgttgac
    13381 catggtaaaa caacattaac agcagcaatc gctactgtat tagcaaaaaa tggtgactca
    13441 gttgcacaat catatgacat gattgacaac gctccagaag aaaaagaacg tggtatcaca
    13501 atcaatactt ctcacattga gtaccaaact gacaaacgtc actacgctca cgttgactgc
    13561 ccaggacacg ctgactacgt taaaaacatg atcactggtg ctgctcaaat ggacggcggt
    13621 atcttagtag tatctgctgc tgacggtcca atgccacaaa ctcgtgaaca cattctttta
    13681 tcacgtaacg ttggtgtacc agcattagta gtattcttaa acaaagttga catggttgac
    13741 gatgaagaat tattagaatt agtagaaatg gaagttcgtg acttattaag cgaatatgac
    13801 ttcccaggtg acgatgtacc tgtaatcgct ggttcagcat taaaagcttt agaaggcgat
    13861 gctcaatacg aagaaaaaat cttagaatta atggaagctg tagatactta cattccaact
    13921 ccagaacgtg attctgacaa accattcatg atgccagttg aggacgtatt ctcaatcact
    13981 ggtcgtggta ctgttgctac aggccgtgtt gaacgtggtc aaatcaaagt tggtgaagaa
    14041 gttgaaatca tcggtttaca tgacacatct aaaacaactg ttacaggtgt tgaaatgttc
    14101 cgtaaattat tagactacgc tgaagctggt gacaacattg gtgcattatt acgtggtgtt
    14161 gctcgtgaag acgtacaacg tggtcaagta ttagctgctc ctggttcaat tacaccacat
    14221 actgaattca aagcagaagt atacgtatta tcaaaagacg aaggtggacg tcacactcca
    14281 ttcttctcaa actatcgtcc acaattctat ttccgtacta ctgacgtaac tggtgttgtt
    14341 cacttaccag aaggtactga aatggtaatg cctggtgata acgttgaaat gacagtagaa
    14401 ttaatcgctc caatcgcgat tgaagacggt actcgtttct caatccgtga aggtggacgt
    14461 actgtaggat caggcgttgt tactgaaatc attaaataat ttctaatttc ttagatttta
    14521 tataaaaaga agatccctca atcgaggggt ctttttttaa tgtgtaaatt ttgtaatggc
    14581 tattcgattt agaagaacaa taattgatga aagactgact aataaaactt ataactgata
    14641 atactgttta aataaaattg ttgagtcttg gacattgtaa aatgctccct tcaaagtttt
    14701 cattttttca atgtctactt tgaagggagc atttcattag tttatgtctc agattcatat
    14761 ctttcaatta atttaaatgc ttaatttgtt ttaaatactt gctctaattc tatgattttt
    14821 aaaaatacag ctacagcgta ttttaatgat ttttcatcaa tatcaaattt gggattatgg
    14881 tgtggcgctg taataccttt actttcatta ccacaaccag tcagaaagaa tgcacctggt
    14941 cgtactttca aataatgtga aaaatcttct ccaatcatca ttaaatctga ttcattaaag
    15001 cgtacatgta agtcatttgt tgcttcttta ataacttgat atgctttctc gttattatgg
    15061 acaggcaaat accctttaat ataattcaaa tcatagttaa tatcatttgc tattgctaaa
    15121 ccttgtagaa gcttatccat tttgtccatt acatgattct gtatatctga atcgaaagtt
    15181 ctaactgtac ctttacaaaa tgcttgatca ggaataacgc tatctgtggt gcctgcttga
    15241 atcattccaa atgaaagtac agcttgttta actggatcga tcgtacgtga aattattttt
    15301 tgtgcactta aaatgaactc tgccatgatt actattgggt caatggtttc atgaggtttg
    15361 gcaccatgac caccacgacc tttaaatgtg acgctaaatt catctggaga ggccatgatt
    15421 gcccccgcac gtgaatgaat agttccagta ggataaccac tccataaatg tgtaccgtaa
    15481 attctatcta cattttccag acatccagca tctatcattt cttgagaacc acctggcatg
    15541 atttcttcac cgtactggaa tattaataca acattacctt ctaataaatg tttatgttca
    15601 tctaaaatct ctgctacagt aagtaaaatt gctgtatgac catcatgccc acacgcatgc
    15661 atacatcctg gatttttaga cttataaggc acatcgttta attcctcgac aggtaacgca
    15721 tcaaagtcag ctcttaatgc aatggtaggt cctgtgccca agcctttaaa tgtggctttg
    15781 ataccattgc ggccgatagg agtttcaata tcacaagata actggcttaa ttggttaaca
    15841 atataatcat gtgtttgaaa ttcttcaaaa gataactcag gatattggtg taaataacgt
    15901 ctgagttgaa ttgttttatt ttctttatta tttgctagtt ggaaccaatc taacaccctt
    15961 atcactactt tctaaaataa tgtttatagt ataacatttt atgaaattat cgtactaaat
    16021 gattgctttg agatatttta tctatgaatg ataaggcttt caagtta
//
LOCUS       DAKTIO010000049        15530 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.049, whole genome shotgun sequence.
ACCESSION   DAKTIO010000049 DAKTIO010000000
VERSION     DAKTIO010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15530
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.049"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..389
                     /locus_tag="O0293_001170"
     CDS             <1..389
                     /locus_tag="O0293_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HCY0280062.1"
                     /translation="TDLIHLNQFQFNFYAGLNLSEWKYFTYLLLGVFLAVYVMILIYF
                     EILTLTSHRWLNRLIVVVLMFLNGFGIYIGRFLRLHSVYFFNEPLHVLNHILESLTLK
                     TAMFVCFMVVMQAAILLFGKGVRLNK"
     gene            386..670
                     /locus_tag="O0293_001171"
     CDS             386..670
                     /locus_tag="O0293_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280063.1"
                     /translation="MTINIIVLIVSIIVFQLIIGHIWHDIGLSYLRSILLMMLPFGLG
                     VFIQQVSYYERQYPKWQVPQNIKVRLKYIYLATFLEYVVLYLTLFTDILR"
     gene            complement(825..2198)
                     /locus_tag="O0293_001172"
     CDS             complement(825..2198)
                     /locus_tag="O0293_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCY0280064.1"
                     /translation="MAIAVLLNRMFRMEHNPLFEYIYQQKEDIDACYFIIPEEDMSSA
                     SDLKAQFYRGTLQRFYQSLHAEKLTPYVMSYDDIISFCKENNISEVVTAGDIMSYHLE
                     EYDILHQRSLFNEARIAVTLIRGNHYFKASKTMNQQGEPYNVFTSFYKKWRPYLRHRD
                     VYHYDLKSFEDFVIASPDDLVFDDIAFGSSQIIEQNKWQHFLDQDIQNYESGRDYLPE
                     VLTSQLSVALAYGLLDIIEIFNDLLARYDEDEANYEAFIRELIFREFYYVLMTQYPET
                     SYQAFKPKYRQIKWSQNEADFNAWCEGQTGFPIIDAAIMELTQTGFMHNRMRMVVSQF
                     LTKDLFIDWTWGEKFFRKHLIDYDAASNIHGWQWSASTGTDAVPYFRMFNPIRQSERF
                     DAKALYIKTYLPIFNQIDAKYLHDTQRNESNLFEQGIELGSHYPRQMVDHQEKRTQVL
                     ATFKALD"
     gene            complement(2284..3837)
                     /locus_tag="O0293_001173"
     CDS             complement(2284..3837)
                     /locus_tag="O0293_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HCY0280065.1"
                     /translation="MSEEKHVVEHEQQKKEKTKKQYKPFWIVMSFIILIVVLLLPAPS
                     SLPIMAKAVLAILAFAVIMWVTEAVSYPVSATLIIGLMILLLGFSPVQNLGEKLGNPK
                     SGSAILAGSDLLGTNHALSLAFSGFATSAVALVAAALFLAAAMQETNLHKRLALLVLS
                     IVGNKTRNIVIGAIIVSIVLAFFVPSATARAGAVVPILLGMIAAFKVSKDSKLASLLI
                     ITSVQAVSIWNIGIKTAAAQNIVAINFINHQLGFDVSWGEWFLYAAPWSIVMSVALYF
                     IMIKVMPPEINTIEGGKDLIKEELHKLGPVSPREWRLIVISMLLLLFWSTEKVLHPID
                     SASITIIALGVMLMPKIGVMTWKHVENKIPWGTIIVFGVGISLGNVLLKTGAAQWLSD
                     QTFGVLGLKHLPIIATIALITLFNILIHLGFASATSLSSALIPVFISLTSTLHLGDQS
                     IGFVLIQQFVISFGFLLPVSAPQNMLAYGTGTFTVKDFLKAGIPLTIVGYILVIVFSM
                     TYWKWLGLL"
     gene            complement(4120..4407)
                     /locus_tag="O0293_001174"
     CDS             complement(4120..4407)
                     /locus_tag="O0293_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280066.1"
                     /translation="MTNEDKRFEQLRFERKFIVIPYLIYAVIVLLLNIFYSDLKITMT
                     LFGLFFAYNVVILFIAFVKHYKRTLLLSLILTVLSGAAFFGIIYVYGINHF"
     gene            complement(4442..5350)
                     /locus_tag="O0293_001175"
     CDS             complement(4442..5350)
                     /locus_tag="O0293_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCY0280067.1"
                     /translation="MEQIMINHYVHFSRLVQGFWRANEWKMTAKELNYFINELVERGI
                     TTMDHADIYGDYQCESLFGNALDLSPELRNKIQIVTKCGIILPSKQFDFTNGHRYDLS
                     SKHIVKSVEQSLINLNVDYLDSLLIHRPSPLMDPEQVADALTKLVKQGKLKSFGVSNF
                     NHSQYQLLNQYIMKERLHISINQLELSPYHVDSLQDGTMDSMYQNHVQIMAWSPFAGG
                     KIFDKEDIKAQRIMKVVQSIADKYGVSDTAVMIAWLVKIPHRIMPILGTSQLKRIDQA
                     IEGLQLNLDDQSWFDIYTAIIVQDIP"
     gene            complement(5453..6379)
                     /locus_tag="O0293_001176"
     CDS             complement(5453..6379)
                     /locus_tag="O0293_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCY0280068.1"
                     /translation="MKNNKDEKIRISIINGFLGSGKTTLLTHYISELLKNDEKIKIIM
                     NEFGTFDIDSNSISNEIEVHSLINGCVCCDLKQELVYELKAIALKGDVNHVIIEATGI
                     AHPLELLVACQDPQIVNFFEKPIIYGVLDATRFLERHQYTENTVSLMEDQLKLSDMII
                     INKIDLITDDSLEKIDKQLGMICASIPTYKTTYGKVSLEELDLTVKDREISSHHHHHH
                     GIKSMTYTFTGPIDRHLFYQFIMKLPESVLRLKGYVSFRDQPNAIYEFQYAYGLPDYG
                     IIGMQLPLTIVIIGETLDTNHIRNQLDMLQFT"
     gene            6606..7049
                     /gene="mgrA"
                     /locus_tag="O0293_001177"
     CDS             6606..7049
                     /gene="mgrA"
                     /locus_tag="O0293_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MgrA"
                     /protein_id="HCY0280069.1"
                     /translation="MSDQHNLKEQLCFSLYNAQRQVNRYYSNKVFKKYNLTYPQFLVL
                     TILWDESPVNVKKVVTELALDTGTVSPLLKRMEQVDLIKRERSEVDQREVFIHLTDKS
                     ETIRPELSNASDKVASASSLSQDEVKELNRLLGKVIHAFDETKEK"
     gene            complement(7176..8849)
                     /locus_tag="O0293_001178"
     CDS             complement(7176..8849)
                     /locus_tag="O0293_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding/permease
                     protein"
                     /protein_id="HCY0280070.1"
                     /translation="MKTRLKFQVDKDLLLAIVVGVCGSLVALAMFFLSGYMVTQSALG
                     APLYALMILVVTVKLFGFLRAITRYVERLISHKATFTMLRDIRVQFFGKLVNVIPNVY
                     RKLSSSDLISRMISRVEALQNIYLRVYYPPVVIGLTALVTVIVLAFISIGHALLIMVS
                     MLFTLLIVPWLSSKKARTLKKHAANEQARFLNHFYDYKAGMDELRRFNQINHYRDNLM
                     AKLNHFDKLQLKEQRFLTIYDFILNIIAMLSIFGSLVLGLIQINAGQLNIIYMTSIVL
                     MVLTLFEQAVPMTNVAYYKADTDQALHDINEVISVPSTNGKKRLNDKYDATNIYEVKD
                     ASFKYWNQQTYVLSDINFNVNRGEKIAIVGPSGSGKSTLLQIMAGLYQLDSGSVRFEN
                     MDMFEIDDKDKFESLNVLLQSQQLFDGTIRQNLFTDEKDEAVQAIFKQLDLEHLALER
                     QIDLDGHTLSGGEIQRLAITRMLLKDTASTWILDEPTTALDKQNSLKVMDLIEAHAET
                     LIVATHDLTLLSRFETIIVMINGKIVEKGNYQQLLANQGALWNMIQYNA"
     gene            complement(8846..10477)
                     /locus_tag="O0293_001179"
     CDS             complement(8846..10477)
                     /locus_tag="O0293_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCY0280071.1"
                     /translation="MKKLTTILFQYKIFPVLMFLVSTGLGIIVITQNILIADFLAKII
                     RHQFQGLWIVLFILLGVLLLRATVQFLNQWLGDTLAFKVKHMLRQRVIYKNNGHPIGE
                     QMTILTENIDGLAPFYKSYLPQVFKSMMVPLIIIIAMFFIHFNTALIMLITAPFIPLF
                     YIIFGLKTRDESKDQMTYLNQFSQRFLNIAKGLVTLKLFNRTEQTEKHIYDDSTQFRT
                     LTMRILRSAFLSGLMLEFISMLGIGLVALEATLSLVVFHNIDFKTAAIAIILAPEFYN
                     AIKDLGQAFHTGKQSEGASDVVFEFLEQPNYNNEFLLKYEENQKPFIQLTDISFRYDD
                     SDRLVLNDLNLEIFKGDQIALVGPSGAGKSTLTHLIAGVYQPTIGTISTNQRDLNIGI
                     LSQQPYIFSASIKENITMFKDIENNTIEEVLDEVGLLDKVQSFTKGINTIIGEGGEML
                     SGGQMRRIELCRLLVMKPDLVIFDEPATGLDIQTEHMIQNVLFQHFKDTTMIVIAHRD
                     NTIRHLQRRLYIENGRLIADDRNISVNITENGDDL"
     gene            10690..11565
                     /locus_tag="O0293_001180"
     CDS             10690..11565
                     /locus_tag="O0293_001180"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCY0280072.1"
                     /translation="MFIIELIKGIILGVVEGLTEFAPVSSTGHMILVDDMWLKSSEFL
                     GSQSAFTFKIVIQLGSVFAAAWVFRERFLEILHIGKHKHVEGDNDQQRRSKPRRLNLL
                     HVLVGMVPAGILGLLFDDFIEEHLFSVPTVMIGLFVGAIYMIIADKYSAKVKNPQTVD
                     QISYFQAFVIGISQAVAMWPGFSRSGSTISTGVLMKLNHKAASDFTFIMAVPIMLAAS
                     GLSLLKHYQDIQIADIPFYILGFLAAFTVGLIAIKTFLHLINKIKLIPFAIYRIVLVI
                     FIAILYFGFGIGKGI"
     gene            11737..12420
                     /locus_tag="O0293_001181"
     CDS             11737..12420
                     /locus_tag="O0293_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280073.1"
                     /translation="MDKKKVIKFMINVLPIVLVPLIVERKRIKQHPDVQKVTDATSKV
                     ASKTSAAISNTASDVKEYVGDKKQDFENKRELKKFAREHDPAYIEKKGEKLAKQNRKD
                     ADKMNKILQKNIEKRHKEEQKAREKNEIQRIKDMKKSQKYEEKAGLTPNKLDEKTEKK
                     GDKLAEKNRKEIAKMNKKLQKNIEKRHKEEQKRQQEADKARIKSFKKYKDYVAKSASQ
                     QNKENNTEA"
     gene            12423..12881
                     /locus_tag="O0293_001182"
     CDS             12423..12881
                     /locus_tag="O0293_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCY0280074.1"
                     /translation="MTHIIIDGDACPVVDSIIDLTTETGIFVTIIRSFSHFSNQLYPP
                     HVSTLYVDDGPDAVDYKIVQLSTKDDIVVTQDYGLASLLVDKVLIVMHHNGKIYNSKN
                     IQQLLDKRYMNAQIRKQGGRHKGPPPFTKQDQKVFEQSLLKVIHRIKELD"
     gene            12883..13449
                     /locus_tag="O0293_001183"
     CDS             12883..13449
                     /locus_tag="O0293_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HCY0280075.1"
                     /translation="MKRIAVYCGASKGHDPSYVQKAYDLGKYFAEQGYELVFGAGSIG
                     IMGAIQDGVLDHGGKAIGVMPKMLDEHEITSQRLTELILVDSMHERKNKMAELADAFV
                     MAPGGAGSLEEFFEMYSWAQIGIHEKPIAIYNINGFFNPLQTMIDHMIEEGFIDPKYR
                     ALAPLCDTKESLIESILNFKPLGTRSYD"
     gene            complement(13545..14087)
                     /locus_tag="O0293_001184"
     CDS             complement(13545..14087)
                     /locus_tag="O0293_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0280076.1"
                     /translation="MIYCETERLILRDWHEDDLLPFQKMNANYDVRKYFPSLLSYRRS
                     ELDMRTMDAVIKDYGIGLFAVEDKESHQWIGFIGLNYIPETSDYPFKELPLYEIGWRL
                     LPEFWGKGLATEGAKATLKLAEEHQIYDVYSFTAEANKASQRVMEKIGMTVYDHFELP
                     NLSKYHLLKRQVRYYINLRK"
     gene            complement(14167..14466)
                     /locus_tag="O0293_001185"
     CDS             complement(14167..14466)
                     /locus_tag="O0293_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280077.1"
                     /translation="MHEQDFRILEGQDITLPELGRELENITGHTIADSTGEIKRVIAH
                     LPNFESDTDTFVATYRLNHQQDFIDATFTALKSDRARLKEVPVHVELISYISKSK"
     gene            complement(14604..14999)
                     /locus_tag="O0293_001186"
     CDS             complement(14604..14999)
                     /locus_tag="O0293_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280078.1"
                     /translation="MKKLIISIMAVMLFLTGCGKSQEKATLEKDIDNLQKENKELKDK
                     KEKLQQEKEKLADKQKDLEKEVKDLKPSKEDNKDDKKDEDKNKDKDKDKEASQDKQSK
                     DQTKSSDKDNHKKPTSADKDQKANDKHQS"
     gene            complement(15066..15509)
                     /locus_tag="O0293_001187"
     CDS             complement(15066..15509)
                     /locus_tag="O0293_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0280079.1"
                     /translation="MRTLNKDEHNYIKQIANIHETLLSQVESNYKCTKLSIALRYEMI
                     CSRLEHTNDKIYIYENEGQLIAFIWGHFSNEKSMVNIELLYVEPQFRKLGIATQLKIA
                     LEKWAKTMNAKRISNTIHKNNLPMISLNKDLGYQVSHVKMYKDID"
BASE COUNT         5269 a         2700 c         2256 g         5305 t
ORIGIN      
        1 taactgattt aatccatttg aaccaatttc aattcaactt ttacgcagga ctaaatttaa
       61 gtgagtggaa atactttacc tatttactgc ttggtgtctt cttagcggtt tatgtgatga
      121 ttttaatcta ttttgaaata ctaacattga cttctcaccg ttggcttaat agattaattg
      181 ttgtcgtgct aatgttttta aatggattcg gaatttatat tggtcgtttc ttacgcttac
      241 attccgttta tttctttaat gagcccttac atgttctaaa tcacattcta gaaagtctaa
      301 cgcttaaaac tgctatgttt gtatgtttta tggttgtcat gcaagcagca attctattat
      361 ttggaaaagg agtgcgtttg aacaagtgac aataaatata atcgttttaa tagtatctat
      421 cattgtcttt caattgatta ttggacacat atggcatgat attggtttaa gttatttaag
      481 aagtatctta ctcatgatgc tgccatttgg actaggtgta tttatacaac aagtgtcgta
      541 ttacgaacga caatatccta aatggcaagt gcctcaaaac ataaaagtta gattgaaata
      601 tatttatctc gctacgtttt tagagtatgt cgtactatac ctcactttat ttacggacat
      661 actaagataa catcaaatta ataagaaaac taaatacaac ttatatattt gaggaaagcc
      721 aatttctatc acatcaataa tagaaattgg cttatttata ttgcttaaga actttatatt
      781 ctgctccaga ttttactcat gccagatcta tcaattgagc cgaattagtc tagcgcttta
      841 aatgtagcta aaacttgtgt acgtttttct tgatgatcta ccatttgtct tggataatga
      901 ctacctaatt caatcccctg ttcaaaaagg ttggactcat tgcgttgtgt atcatgcaaa
      961 tattttgcat caatttgatt aaaaatcgga agatatgttt tgatatacaa agctttagca
     1021 tcaaagcgtt cactctgtct tattggatta aacattctaa aatacggcac tgcatccgta
     1081 cctgtagaag cagaccattg ccatccatga atatttgatg ctgcatcata gtcaataagg
     1141 tgctttctaa agaatttttc tccccatgtc caatctataa ataaatcttt ggttaaaaat
     1201 tgcgacacaa ccattctcat tcgattatgc ataaaaccag tttgtgtcaa ttccattatt
     1261 gctgcatcaa tgattggaaa tcctgtttgc ccttcgcacc atgcattaaa atccgcttca
     1321 ttttgcgacc attttatctg tcgatattta ggtttgaaag cttggtatga ggtttcagga
     1381 tactgtgtca ttaacacata ataaaattct ctaaaaatga gttcacgtat aaatgcttca
     1441 tagtttgcct catcttcatc ataacgcgcc aataaatcat taaaaatttc aataatatct
     1501 aataatccat atgctaaagc aacacttagc tgacttgtta atacttcagg taaatagtct
     1561 cttccgcttt cgtaattctg tatatcttga tctaaaaaat gttgccattt attctgttca
     1621 attatttgtg aggatccaaa tgctatgtca tcaaacacta aatcatcagg tgatgcaatg
     1681 acaaagtctt cgaatgattt taaatcatag tgatatacgt ctctatgcct caagtaaggt
     1741 cgccattttt tatagaaact agtaaaaaca ttgtatggct ccccttgttg attcattgtt
     1801 ttactcgctt taaagtaatg attcccacgt atcaaagtaa cggcaatgcg tgcttcattg
     1861 aataaagaac gttgatgtaa aatatcatat tcttcaagat gataactcat aatatcaccc
     1921 gcagtcacta cttcagagat attgttttct ttacaaaatg aaatgatatc gtcataagac
     1981 ataacataag gtgtaagctt ttctgcgtgc aacgattggt aaaagcgctg caaagtaccg
     2041 cgataaaact gtgctttcaa atcagaagct gaagacatgt cctcttccgg aatgataaaa
     2101 taacatgcat caatgtcttc tttttgttga taaatatatt caaataatgg attgtgttcc
     2161 attcgaaaca ttcgatttaa taacacagca attgccataa acaaaacacc cttcaattat
     2221 ctttttattt tattaagctt tttgtcactt taaaataacc tagattctta tttatatttt
     2281 taattaaagc aaacctaacc atttccaata agtcatgcta aaaactatca ctagaatata
     2341 ccctacaatt gtcaatggta tacctgcctt caagaaatct ttaaccgtaa aagtaccagt
     2401 gccataagcc aacatatttt gaggtgcact aacaggtaat aagaaaccaa aactaataac
     2461 aaattgttga attaaaacaa atcctataga ctggtctcct aagtgtaacg tagaggttag
     2521 cgaaataaaa acaggtatta acgctgatga taaacttgtt gcactcgcaa agcccaaatg
     2581 aatcaatata ttaaaaagcg tgataagcgc aattgtcgcg ataataggta aatgttttaa
     2641 acctaaaaca ccaaaagttt gatcacttaa ccattgagct gcacctgttt tcaaaagaac
     2701 gttacctagt gaaataccta caccaaacac gataattgtt ccccatggta ttttattttc
     2761 aacatgtttc catgtcatga caccaatttt cggcattaac ataacaccta aagcaataat
     2821 agtaatggat gcagagtcaa tcggatgtaa tactttttca gttgaccaaa acagtaataa
     2881 taacatcgat ataacaatta aacgccattc acgtgggcta acggggccaa gtttatgcaa
     2941 ttcttctttt attaaatctt taccaccttc tattgtatta atttctggag gcatcacttt
     3001 aatcatgatg aaatataaag ctacggacat aactatggac caaggcgctg catataagaa
     3061 ccactcgccc catgaaacat caaatcctaa ttgatggttt ataaaattaa tcgctacgat
     3121 attttgtgct gccgccgttt tgataccaat attccaaatt gacacagctt gtactgaagt
     3181 aattattaat aaagacgcta acttgctatc tttggaaact ttaaatgccg caatcatacc
     3241 cagcaagatt ggtacaactg cccctgctct agctgttgca gaaggaacga aaaatgcaag
     3301 tacaattgaa acgataattg ctccaataac tatatttcta gttttattac caacaattga
     3361 taacactaaa agagctagtc ttttatgcaa attcgtttct tgcatagcag cagccaaaaa
     3421 taatgcagct gcaacgagag ctacagctga agttgcaaat ccactaaacg ctaatgataa
     3481 tgcatgatta gttcctagaa ggtcacttcc agctaaaata gcactgccac ttttcggatt
     3541 acctagcttc tcccctaaat tttgaacagg gctaaatcct aaaagtaata tcattaagcc
     3601 aataattaaa gttgctgaca ccggatatga tacagcttcc gttacccaca taataactgc
     3661 aaaagctaaa atagctagta ctgccttagc cattatcggc agacttgaag gtgccgggag
     3721 taatagtaca actataagta ttataaaact catgacaatc caaaatggct tgtattgctt
     3781 ttttgtcttt tctttctttt gttgttcatg ttcaactaca tgtttttctt ctgacataac
     3841 agtcacccct ttgtttaatc ttaatttaat tataacaatt ttttaaaaca tacattttta
     3901 atttatgcaa aatacacaat tttatcgaaa tattttgtaa ataatattgc taggtgatga
     3961 gttatctcgg ttaattacaa aacgatatct aagtttaaat attagatcat aaaaaaacta
     4021 tctactgaat atcctcatag catcattttt ctaagcaaaa gttaaactta gttagataaa
     4081 atgcaaatga gattattgta gatagtctct ttttaatgtt taaaaatgat taatgccata
     4141 aacataaata attccaaaga atgccgcgcc actaagcact gttaatataa gacttagtaa
     4201 caatgtgcgt ttataatgtt taacaaatgc tatgaacaaa atgactacat tatacgcaaa
     4261 gaaaagtccg aataatgtca ttgttatttt caaatcagaa tagaaaatat ttaatagtaa
     4321 tacaatgact gcataaatta aatacggaat aactataaat ttgcgttcaa atcttaattg
     4381 ttcgaaacgt ttatcttcgt ttgtcatgat atgctccttt atgatttaaa agtaaataag
     4441 tttacggaat atcttgtacg ataatagcgg tgtaaatgtc aaaccacgac tgatcatcta
     4501 aattaagttg tagcccttcg attgcttgat caatacgctt taactgactt gttccaagta
     4561 tcggcatgat acgatgcggt atttttacta accacgctat catcacagct gtgtcactca
     4621 caccatattt gtcagctatt gattgaacaa ctttcataat acgttgcgct ttaatatctt
     4681 ccttgtcgaa aattttaccg cctgcaaaag gactccaagc catgatttga acatggtttt
     4741 gatacattga atccattgtt ccatcttgta aactatcaac gtgatatggc gataattcta
     4801 attgattgat gctaatatgt agtctttctt tcataatata ttgatttaac aattggtatt
     4861 gtgaatgatt aaaattcgac accccgaatg acttcaactt accttgttta acaagtttag
     4921 ttaatgcatc agcaacttgt tctggatcca tcaatggtga aggacgatga atgagtagac
     4981 tatctaaata atctacattc aaattgatta atgactgttc aacagatttc acgatgtgct
     5041 tactactcaa atcataacga tgtccatttg taaaatcaaa ttgcttagaa ggcaaaatga
     5101 taccacattt cgtaacaatt tgaattttat ttcttaattc gggtgataaa tccaaagcat
     5161 taccaaacag tgattcacat tgataatctc cataaatatc agcatgatcc atcgttgtaa
     5221 ttccacgttc aactaattca tttataaaat aatttaactc tttcgcagtc atcttccatt
     5281 catttgcacg ccaaaaacct tgtacaagcc tagaaaaatg aacatagtga ttaatcatta
     5341 tttgttccat atctcatcca ctccatccct caatgtcaca aaatattcac aataaaagta
     5401 ttaagaaatt aatgtgctat ttccgttaaa ttgaaacaac acattttctg atttacgtaa
     5461 attgtagcat atccaattga ttacgtatgt gatttgtatc taaagtttca ccaataataa
     5521 caatcgttaa tggtaattgc atgccaatta ttccatagtc tggtaaacca tatgcatatt
     5581 gaaattcata aattgcattt ggttgatctc taaatgacac ataacctttc aaacgtagaa
     5641 cagattccgg taatttcatt ataaattgat aaaacaaatg acgatcaatc ggacctgtaa
     5701 acgtgtaagt catactttta atcccatgat gatggtgatg atgagacgat atctctctgt
     5761 ctttaacagt taagtccaat tcttccaacg aaacttttcc ataggttgtt ttataagttg
     5821 gaatacttgc acaaatcata cctaattgct tatcaatttt ctcaagactg tcatcagtta
     5881 taagatcaat tttattaata ataatcatgt cacttagttt caactgatct tccatcagcg
     5941 aaactgtatt ttcggtatat tgatgacgtt ctaaaaatcg agtcgcatct aatacaccat
     6001 aaataatcgg cttttcaaag aaattaacga tttgcggatc ttgacatgca actagtaatt
     6061 ccaaaggatg cgcaatgcct gtcgcttcta tgatgacatg attaacgtcc ccttttaaag
     6121 caatggcttt tagttcatag acaagttctt gtttaagatc gcaacaaaca caaccattaa
     6181 tcaatgaatg gacttcaatt tcatttgaaa tactattgct atcaatatca aaagtaccga
     6241 attcattcat gatgatttta attttctcat cattttttaa taattcacta atataatgtg
     6301 tcagtaacgt ggttttacca ctacccaaaa atccgttaat tatggatatt cttatttttt
     6361 catctttatt atttttcatt ttatattctc ttttctatta cagttatttt taaaaatgat
     6421 aacatatata ttaaattatc tttttgcttg atttaatcaa aaatatcttc taaactttaa
     6481 acatacttta attttagcat ggcattgaat caaagaaagt tgtgaattaa taaacaatca
     6541 actttttaat gattaccaat ccgaaaagag aaaaaaacgg atagtatgtc tggaggagaa
     6601 ctttaatgtc tgatcaacat aatttaaaag aacagctatg ctttagtttg tacaatgctc
     6661 aaagacaagt taatcgctac tactctaaca aagtttttaa gaagtacaat ctaacatacc
     6721 cacaatttct tgtcttaaca attttatggg atgaatctcc tgtaaacgtc aagaaagtcg
     6781 taactgaatt agcactcgat actggtacag tatcaccatt attaaaacga atggaacaag
     6841 tagacttaat taagcgtgaa cgttccgaag tcgatcaacg tgaagtattt attcacttga
     6901 ctgacaaaag tgaaactatt agaccagaat taagtaatgc atctgacaaa gtcgcttcag
     6961 cttcttcttt atcgcaagat gaagttaaag aacttaatcg cttattaggt aaagtcattc
     7021 atgcatttga tgaaacaaag gaaaaataat taacttttgt catgacaatt aaagtaatgt
     7081 ttagaattta ttaagaatag aaaaacaatt agcacgcgta agcttgttag ttaaaaaact
     7141 gcttgaaagg tttcttagcc tatcaagcag tttttttatg cattatattg aatcatattc
     7201 cataaagcac cttgattagc tagtaattgt tgatagtttc ccttttcaac tattttacca
     7261 tttatcatca caatgatggt ctcaaaacgt gacaataaag ttaaatcgtg tgtagcaaca
     7321 attaatgttt ctgcatgtgc ttcaattaaa tccataactt ttaaactatt ttgtttatct
     7381 aatgcagttg ttggttcatc taaaatccat gttgatgcag tatcttttaa taacatcctc
     7441 gtaatcgcta aacgctgaat ttctccgcca gataatgtat gaccatctaa gtcaatttga
     7501 cgttctagtg ccaaatgttc taaatctaat tgcttaaata ttgcttgcac cgcttcatct
     7561 ttttcatcgg taaataaatt ttgacgtatt gtaccatcaa ataattgttg agattgtagc
     7621 aagacattta acgattcaaa cttatctttg tcatctattt caaacatatc catattttcg
     7681 aaacgaacag agccactatc taattgatat aaccctgcca taatttgtag taatgtactt
     7741 tttcctgaac cagaaggacc cacaatcgca atcttttcgc ctctattaac attaaaatta
     7801 atatccgaca acacatacgt ttgctgattc caatacttaa aactagcatc cttaacttca
     7861 taaatgttcg ttgcatcata cttatcatta agacgttttt ttccattagt agaaggtaca
     7921 gatatcactt cattaatatc gtgcaatgct tggtcagtat ccgctttata atacgcgaca
     7981 tttgtcattg gtacagcttg ttcaaataaa gttaagacca ttaaaactat actcgtcata
     8041 taaataatat ttagttggcc tgcattaatt tgaattaatc ctagaactaa actaccaaaa
     8101 atcgaaagca tagcaataat atttaatata aaatcataaa tcgttaaaaa gcgttgctct
     8161 ttaagttgta atttatcaaa atgatttaat ttagccatca aattatctcg ataatgatta
     8221 atttgattaa atcgacgtag ttcatccata ccagctttat aatcataaaa atgatttaaa
     8281 aatcgggcct gttcattagc tgcatgtttc tttaaagtac gtgctttttt tgagcttaac
     8341 caaggaacaa tgagtaaagt gaacaacatg ctaaccataa tcaatagcgc atggccgatt
     8401 gaaatgaacg ccaaaactat gactgtaact agcgctgtca aaccgatgac gactggtgga
     8461 taataaacac gtaaatatat attttgtaat gcctcaacac gactaatcat acgtgaaatt
     8521 aaatcactag aactcagttt acggtaaaca ttaggaatga catttactaa tttaccgaaa
     8581 aactgtaccc gaatatcacg tagcattgta aatgtagctt tatgagaaat aaggcgctct
     8641 acgtatcgag taatagctct taaaaaccca aacaatttta ctgtaacgac taaaatcatc
     8701 agagcgtata gtggcgcacc aagtgcactt tgtgtcacca tataaccact taagaaaaac
     8761 atggcgagcg caactaaact tccacaaaca ccaacaacta tagctaacaa taaatcctta
     8821 tctacttgaa attttagtcg tgttttcata agtcatcacc attttctgtt atatttactg
     8881 aaatattgcg atcatcagca atcagtcttc cattttctat atacaagcgt cgttgtaaat
     8941 ggcgaattgt attatctcta tgtgcaatga caatcatcgt tgtatcttta aaatgttgaa
     9001 acagaacgtt ctgaatcatg tgttctgttt gaatatctaa accagttgca ggctcatcaa
     9061 atataacgag atctggcttc ataactaaaa gacggcaaag ttctatgcgt ctcatctgtc
     9121 caccagataa catttcgcct ccttcaccta ttattgtgtt aatgcctttt gtgaaagatt
     9181 gcactttgtc taataaacct acttcgtcta gcacttcttc aatagtatta ttttctatat
     9241 ctttaaacat cgtaatattc tcttttatag aagcactgaa aatatatggc tgttgactaa
     9301 gtattcctat atttaaatca cgctggtttg tacttatagt acctattgtt ggctgataaa
     9361 cacctgcaat aagatgtgtc aaagtggatt tacctgcccc gcttggacct acaagtgcaa
     9421 tttgatcacc tttaaatatt tccaaattta aatcatttaa taccaatcta tcagaatcat
     9481 catatcgaaa tgatatgtct gttaactgaa taaatggctt ttggttttcc tcatacttta
     9541 atagaaattc attattatag ttcggttgtt ctaaaaactc aaacacaacg tcactggcac
     9601 cttcactttg ttttccagta tggaacgctt gccctaagtc cttaattgca ttataaaatt
     9661 caggcgctaa aataatcgca attgccgcag ttttaaaatc aatattatga aatactacta
     9721 agcttagcgt tgcttccaat gcaaccaatc caatacctaa catacttata aattcgagca
     9781 ttaatcccga taaaaaagca ctgcgtaaaa tgcgcattgt taaagttcta aactgagtac
     9841 tatcgtcgta aatatgcttc tctgtttgct ctgtacgatt aaatagcttt aacgtcacta
     9901 aacctttagc aatatttaaa aaccgttgac taaattgatt caaataagtc atttgatctt
     9961 ttgactcatc tcgcgttttc aaaccgaaaa taatataaaa caaaggaata aatggtgcag
    10021 ttattaacat aattaatgcg gtattgaaat ggatgaaaaa cattgcaatg attatgatga
    10081 gcggaaccat cattgatttg aacacttgag gcaaataact cttataaaaa ggtgctagac
    10141 catcaatgtt ttctgtgagt atagtcattt gttcaccgat tggatgacca ttatttttat
    10201 aaataacccg ctgtctaagc atatgcttaa ctttaaatgc taatgtatca cctaaccatt
    10261 gatttagaaa ttgcacagtt gctcttaaaa gtaaaacacc taataaaata aataatacaa
    10321 tccataaacc ttgaaattga tgtcttataa ttttagctaa aaaatctgct attaaaatat
    10381 tttgcgttat aacgattatg ccgagaccag tactgaccaa gaacatgagt accggaaaaa
    10441 ttttatattg aaacagtatt gttgttaatt ttttcacaat tatatcacct aaacctatta
    10501 taaaaggttt tccttaaaat caaattaaat agctcaataa aatattgtag ttttacatgt
    10561 aaaagaaaat gaatatatac atctttagtc tagtattata actagcatgt ctttaaatga
    10621 atatgctatc atgatatggt aattttgata tttaaataaa ttaaattgag taatgaaagt
    10681 gggtacatta tgtttatcat tgaattaata aaaggtatta tcttaggggt cgtcgaagga
    10741 ttaacagaat ttgcacctgt ttcctctact ggacatatga tcctagttga tgatatgtgg
    10801 ttaaaatcat ctgaattttt aggttctcag tcagcattta catttaaaat cgtcatccaa
    10861 ttaggatccg tctttgcagc agcatgggtg ttccgcgaac gcttcttaga gattttacat
    10921 attggtaaac acaaacatgt tgaaggagat aacgatcaac aaagacgttc aaagccaaga
    10981 cgtttaaatt tattacatgt attagtgggt atggtgccag caggtatttt aggcttacta
    11041 tttgatgatt tcatcgaaga acatttattt agtgtgccaa ctgttatgat tggcttattc
    11101 gtaggtgcta tctatatgat tattgctgat aaatactcag ctaaagttaa aaacccacaa
    11161 acagtagatc aaatcagtta tttccaagca tttgtaattg gtatttctca agcagtagct
    11221 atgtggcctg gtttcagccg ttcaggctca acaatttcaa ctggggtatt aatgaaatta
    11281 aatcataaag cagcatcgga ctttacattt attatggctg ttccaattat gttagcagct
    11341 agtggtttat ctttattaaa acattatcaa gatattcaga ttgctgatat acctttttat
    11401 attttaggct ttttagctgc atttactgtt ggactaattg caatcaaaac attcttgcat
    11461 ctaattaaca aaatcaaact aattccattt gccatttaca gaattgtatt agtaatcttt
    11521 attgctatac tttacttcgg atttggcatt ggtaaaggta tttaatttcc ttttaaaata
    11581 tagactgtca attaaggaat cacttgattg gcagttttta ttgtcttctt ttaaaaaaca
    11641 tctgaaatac ttaatagtga ttcactacta tcatcgttta tattgatgta tcatagggta
    11701 tgtaaaatta ataaagtaat tatggaggca agatctatgg ataagaaaaa agtcatcaaa
    11761 tttatgatta atgtattacc aattgtattg gtaccgttaa ttgttgaacg taaacgtatc
    11821 aaacaacatc cggacgtaca aaaagttaca gatgctacaa gtaaagttgc ttcaaaaaca
    11881 tctgcagcaa tcagtaacac agcgagtgat gttaaagaat atgtcggcga taaaaaacaa
    11941 gattttgaaa ataaacgtga acttaaaaag tttgctagag aacatgatcc tgcctatatt
    12001 gagaaaaaag gcgaaaaatt agctaaacaa aatcgtaaag acgctgataa aatgaataaa
    12061 atacttcaaa aaaatatcga aaagcgtcat aaagaagagc aaaaagcccg cgaaaagaat
    12121 gaaatacaac gtattaaaga tatgaaaaaa tcacaaaaat acgaagaaaa agcaggctta
    12181 acacctaata aattagatga gaaaactgag aaaaaaggcg ataaactagc tgaaaaaaat
    12241 cgcaaagaaa tcgctaaaat gaataaaaag ttacaaaaaa atattgaaaa acgacacaaa
    12301 gaagaacaaa aacgccaaca agaagctgat aaagcacgca tcaagtcatt taaaaaatat
    12361 aaagattatg ttgccaaaag cgcctctcaa caaaataaag aaaacaatac agaggcataa
    12421 ttgtgacaca tattattatt gatggagatg cttgtcctgt tgttgattct attatagatt
    12481 taacaactga gacaggcatt tttgtgacaa ttattcggag cttcagccat ttttcgaacc
    12541 aattatatcc tccacatgta tcaacattat atgttgatga tggaccagat gcagttgatt
    12601 acaaaattgt tcaattatca acgaaggatg atatcgtagt cactcaagat tatggactcg
    12661 caagtttatt agtcgacaaa gtcttaattg tcatgcatca taatgggaag atatacaatt
    12721 ccaaaaatat tcaacaacta ttagataaac gatatatgaa tgcacaaatt agaaaacaag
    12781 gtggtcgcca caaaggtccc ccacctttta caaagcaaga tcaaaaagtg tttgaacaat
    12841 cactattaaa agtcattcat cgaattaagg agctggatta acatgaaacg aatcgcagtt
    12901 tattgtggtg caagcaaagg tcatgaccct tcatatgtac aaaaagcata cgatttaggt
    12961 aaatattttg ctgaacaagg ttatgaatta gtatttggtg cagggtcaat tggcattatg
    13021 ggtgccattc aagatggtgt attagatcat ggcggcaaag ctattggcgt catgcctaaa
    13081 atgttagatg aacatgaaat cacaagccaa cgtttaactg aattaatttt agttgattct
    13141 atgcatgaac gtaaaaataa aatggcagaa cttgcagatg catttgtcat ggctcctggt
    13201 ggcgcaggtt cactagaaga atttttcgaa atgtatagtt gggctcaaat aggtatccat
    13261 gaaaagccta ttgccattta taatattaat ggctttttca atccactaca aacaatgatt
    13321 gaccatatga ttgaggaagg ttttattgat cctaagtatc gtgctcttgc accgttatgc
    13381 gacactaagg aatcattaat tgaatcaata ttaaatttca aacctctggg tactcgttca
    13441 tacgattaat cattctaagg ttaaaaaata aacaaacacc cctaatattg ttatcatcag
    13501 ctaaacaatg ttaggggtgt ttgttacgct tatgtataaa tttttcactt tcgaagatta
    13561 atgtaatagc gcacttgcct ttttaataaa tgatacttac ttagattggg taattcgaaa
    13621 tgatcataca ctgtcatgcc aattttttcc attacacgtt gtgaagcttt atttgcttct
    13681 gctgtaaaac tatagacatc gtatatttga tgttcttctg ctaacttcaa tgttgccttt
    13741 gcgccttcag ttgctaatcc ttttccccaa aattctggca acaagcgcca acctatttca
    13801 taaagcggta attctttaaa tggataatcg cttgtttctg gaatataatt caaacctata
    13861 aagcctatcc attgatggga ctctttatct tctacagcaa ataatccaat gccataatct
    13921 ttaataaccg catccatagt tctcatatct aattctgaac gacgataact caataaactt
    13981 ggaaaatatt tacgtacgtc ataattcgca ttcatttttt gaaaaggtaa cagatcatct
    14041 tcatgccagt ctcttaatat taaacgctct gtttcacaat aaatcatcga ttgacactcc
    14101 tatataattt ctcaataaaa aacacaaaat cgatattttg atcaactttg cgttttagat
    14161 agcagtttat tttgatttag aaatataact tataagttca acatgaactg gcacttcttt
    14221 taaacgtgct ctatctgatt tcagcgcagt aaaagttgca tctataaaat cttgttgatg
    14281 gtttaaacga tatgtagcaa caaaagtatc tgtatcggac tcaaagtttg gtaaatgtgc
    14341 aattacacgc ttaatttcgc cagtagaatc agcaatcgta tgtcctgtaa tattttctaa
    14401 ttctctacct aattctggca aagtaatatc ttgaccctct aaaattctaa aatcttgttc
    14461 atgcatgccg tttctctcct ttattaatta cgttgtctat ttttacgttc tgtttcaatc
    14521 atgaccacat tttaattaat acataccctt aaaataaggt ttaaaacgct gtacctttca
    14581 tctataacaa gtaagcaatt cgattatgat tggtgtttgt cattagcttt ttgatcttta
    14641 tctgctgatg taggcttttt gtgattatct ttatccgatg acttagtttg atcttttgat
    14701 tgcttatctt gtgatgcctc tttatcttta tctttgtctt tatttttgtc ttcgtctttt
    14761 ttatcatcct tgttatcttc ttttgaaggt tttaaatctt tcacttcttt ttcaaggtct
    14821 ttttgcttat ctgctaattt ttctttttct tgttgaagct tttctttttt gtcttttaat
    14881 tctttatttt ctttttgtaa attatcgata tccttttcca gagtggcttt ctcttgactt
    14941 ttaccacaac ctgttaaaaa tagcatgacc gccataatac tgatgattaa tttcttcata
    15001 taaaactcct taacgtcatt aaatcttttt taattttaac atgaattagc aacataatcc
    15061 taattctaat caatatcttt atacattttc acatgactca cttgataacc taaatcttta
    15121 ttcaaagata tcattggcaa attattttta tgaattgtat tgcttattcg ctttgcattc
    15181 atagtttttg cccatttttc aagcgcaatc ttcagttgcg tagctattcc cagtttgcga
    15241 aattgtggtt caacatatag cagttcaatg ttaaccatac ttttttcatt actaaaatgt
    15301 ccccaaataa acgctattaa ttgaccttca ttttcatata tataaatttt atcatttgta
    15361 tgttctaatc ttgaacatat catctcgtac ctaagagcaa tactcagttt agtacattta
    15421 tagttggatt ctacttgcga caataatgtc tcatgtatat tagctatttg cttgatataa
    15481 ttatgttcat ctttattaag tgtcctcatt ttcctctcca atattaatcg
//
LOCUS       DAKTIO010000050        15457 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.050, whole genome shotgun sequence.
ACCESSION   DAKTIO010000050 DAKTIO010000000
VERSION     DAKTIO010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15457
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.050"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..549)
                     /locus_tag="O0293_001188"
     CDS             complement(<1..549)
                     /locus_tag="O0293_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP synthase"
                     /protein_id="HCY0280080.1"
                     /translation="MIPTLEEIIDKYGNLVDYLKFDVTVEVYEDLLLLRSLSDLNEHQ
                     KMDRVSFIKKHLNSNFDYLLDDTEFRSINKIYSNLNIMTHINSQNNNFKRNPFINEEQ
                     LKSLLAIKKVDAHMSYDSNITSKALAEINKTQKRLVTKIDTLYNQSKKENEYVRLGEK
                     ISYTKLENHWKYLYNEIQFYNLN"
     gene            688..978
                     /locus_tag="O0293_001189"
     CDS             688..978
                     /locus_tag="O0293_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280081.1"
                     /translation="MNKKSKQQEKLYNFIIAKSFQQPVGSTFTYGELRKKYNVVCSTN
                     DQREVGRRFAYWIKYTPGLPFKIVGTKNGSLLYQKIGINPCNNSTPSKGGDC"
     gene            978..2765
                     /locus_tag="O0293_001190"
     CDS             978..2765
                     /locus_tag="O0293_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HCY0280082.1"
                     /translation="MSYTLFEIGPFALDSFGWKETIPPKKDGDSEKVVRMASPIIVNA
                     RFSDPITGVEKLIITNNNGKKDIFESDILTTRNLPSLIKYGYSINEKYIRSLSYALQL
                     MRDRLPLSELYEGVGILETPFGYLISLDKVLKSIQFNQSSPSYPIVDSAYDLTPKGTF
                     DNWFNMYIDEVKGHLLLELAVIFGISALVTSFLKHKHEIEFAGILFSFTGQSSTGKST
                     AAALAVSVAGNPTKGNETLFRSWNATRNALEGYLSNNYGIPIVFDELSSTTLRDTTGL
                     LYSIAEGQGRQRSNVHGEVKTPKNWGTSVISTSEYSIFNDSAQNDGLRVRTIEINEQF
                     TTNATNADNIKKAVALNYGHVLPLVAKYLINREDEVIQWFYKEVDWFEAKLKDETNNT
                     GIRMFKRYAVITTSAKILGRVLSTDIDIANIRDYFIDYHTHTVSERSLADKAIDVIIQ
                     FVAQNRGKFSDEGALKNMFENYGLISLKDDHIEVKIIANVFKQMLNNHQFQDVNNVVN
                     ALRDKGFILADRGRQTTKRSVKDNSGKKQSLVFYHLKLDVEFASILGLTKDKSLLQNW
                     TPSNDNKAAKELFKSANEGIGPSGVHEDF"
     gene            2999..4348
                     /locus_tag="O0293_001191"
     CDS             2999..4348
                     /locus_tag="O0293_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCY0280083.1"
                     /translation="MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQ
                     SGRKDNRSGYRQITQLIQQGQCDILCCYRLNRLHRNLKNALKLIKLCQTYHVHILSVH
                     DGYFDMDQAFDRLKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
                     NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYC
                     GRVNNQYGQFDNMFPSIVSTSIYEQAQRLRLQKQTKQTPSDNQLKQKIKCPCCNATLT
                     NMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEVCRDFYQNQRIYTKI
                     KSAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSING
                     HQIQHIIQNIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA"
     gene            4370..5998
                     /locus_tag="O0293_001192"
     CDS             4370..5998
                     /locus_tag="O0293_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCY0280084.1"
                     /translation="MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFE
                     VKDIYADRGISGKSMNRPELQRMLKDAKEGNIDCVMVYKTNRLARNTSDLLKIVEDLH
                     KQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
                     LPLGYDKIPDSKHELMINQHEANIVKYIFECYAKGHGYRKIANALNHKGYVTKKGKPF
                     SISSITYILANPFYIGKIQFAKYKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQ
                     MRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKDGTKKRIRYYSCSNF
                     RNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVTHVNQENQVDGAALHHDI
                     AYKQQQYDEVQIKINNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITNQINQLKNQQNE
                     DKTLFDAKEISKLLQHIFHDIKHIEKSRLKALYLSVIDRIDIKKDGNHKKQFYVTLKL
                     NNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI"
     gene            6520..6870
                     /locus_tag="O0293_001193"
     CDS             6520..6870
                     /locus_tag="O0293_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002502216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280085.1"
                     /translation="MKTITQELKQYITHLFQLSNNEAWECEALEEAAENILPERFIND
                     SPLVHLTLETYTYYNDELHELSIYPFLMYANNQLISVGYLDHFDMDFLYLTDTQNIII
                     DERHLLKQGGQDHE"
     gene            6957..7268
                     /locus_tag="O0293_001194"
     CDS             6957..7268
                     /locus_tag="O0293_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HCY0280086.1"
                     /translation="MKINRYITRGINESIPLDLQILLWHMVEKKDNQPHTDYLHIFKL
                     QEDENILSITHEQEQPTYKLEYHYTNYEKNQNALPKKVYVIREDDVDAFYYVMLLPEE
                     Y"
     gene            7283..7789
                     /locus_tag="O0293_001195"
     CDS             7283..7789
                     /locus_tag="O0293_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HCY0280087.1"
                     /translation="MNTIKSTIHTEAIFSDDEQHRYLLKKTWDEKKAACTVITMYPHL
                     DGVLSLDLTTVLILNQLANSERYGAVYLVNLFSNIRTPENLKHIKNPYDEHTDIHLMK
                     AISESDTVILAYGAYAKRPVVIDRVEQVMEMLKPHKKKVKKLINPVTNEIMHPLNPKA
                     RQKWILKS"
     gene            7841..9448
                     /locus_tag="O0293_001196"
                     /pseudo
     CDS             7841..9448
                     /locus_tag="O0293_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274780.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(9439..9699)
                     /locus_tag="O0293_001197"
     CDS             complement(9439..9699)
                     /locus_tag="O0293_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HCY0280088.1"
                     /translation="MYVVYRASHETAKEALGDKELRAIAHELTKTVKDNMSVDWSKRD
                     SAKAKMRVQVRRLLKKYGYPPDLQKMAVEQVVEQAELMASQQ"
     gene            complement(9671..10657)
                     /locus_tag="O0293_001198"
     CDS             complement(9671..10657)
                     /locus_tag="O0293_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer MecR1"
                     /protein_id="HCY0280089.1"
                     /translation="MLSSFLMLSIISSLLTICVIFLVRMLYIKYTQNIMSHKIWLLVL
                     VSTLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVTKDLAINVNQFET
                     SNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERLKVCQSKMQFYKK
                     HITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHELSHVKSHDLIFNQ
                     LYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRYGESILKCSILKS
                     QHINNVAAQYLLGFNSNIKERVKYIALYDSMPKPNRNKRIVAYIVCSISSFT"
     gene            10754..12763
                     /gene="mecA"
                     /locus_tag="O0293_001199"
     CDS             10754..12763
                     /gene="mecA"
                     /locus_tag="O0293_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase MecA"
                     /protein_id="HCY0280090.1"
                     /translation="MMKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFK
                     QVYKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKI
                     KTNYGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNN
                     VELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTV
                     KKMDEYLSDFAKKFHLTTNETESRNYPLGKATSHLLGYVGPINSEELKQKEYKGYKDD
                     AVIGKKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQ
                     KSIYNNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPL
                     LNKFQITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV
                     NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKN
                     LDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISK
                     ENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKD
                     NPNMMMAINVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
     gene            complement(12809..13237)
                     /locus_tag="O0293_001200"
     CDS             complement(12809..13237)
                     /locus_tag="O0293_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HCY0280091.1"
                     /translation="MKYDDFIVGETFKTKSLHITEEEIIQFATTFDPQYMHIDKEKAE
                     QSRFKGIIASGMHTLSISFKLWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAE
                     ITNKKSIKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
     gene            complement(13334..14077)
                     /locus_tag="O0293_001201"
     CDS             complement(13334..14077)
                     /locus_tag="O0293_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HCY0280092.1"
                     /translation="MLTVYGHRGLPSKAPENTIASFKAASEVEGINWLELDVAITKDE
                     QLIIIHDDYLERTTNMSGEITELNYDEIKDASAGSWFGEKFKDEHLPTFDDVVKIANE
                     YNMNLNVELKGITGPNGLALSKSMVKQVEEQLTNLNQNQEVLISSFNVVLVKLAEEIM
                     PQYNRAVIFHTTSFREDWRTLLDYCNAKIVNTEDAKLTKAKVKMVKEAGYELNVWTVN
                     KPARANQLANWGVDGIFTDNADKMVHLSQ"
     gene            complement(14914..15285)
                     /locus_tag="O0293_001202"
                     /pseudo
     CDS             complement(14914..15285)
                     /locus_tag="O0293_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016912861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase family
                     protein"
     gene            15339..>15457
                     /locus_tag="O0293_001203"
     CDS             15339..>15457
                     /locus_tag="O0293_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCY0280093.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYTLSYRDISEILRERGV"
BASE COUNT         5645 a         2590 c         2386 g         4836 t
ORIGIN      
        1 attaagatta taaaactgaa tctcattata taaatatttc caatgattct ccaattttgt
       61 atacgatatt ttttctccta accgtacata ttcattttct ttttttgact gattatagag
      121 tgtgtctatc tttgtaacta atcttttttg cgttttgttt atttctgcca atgcttttga
      181 agtaatattt gaatcataac tcatatgtgc atctactttc tttatcgcga gtagtgattt
      241 caattgttct tcatttataa atggattacg tttaaaatta ttattttgac tattgatatg
      301 cgtcatgata tttaaattac tgtaaatctt attgatactt ctaaactcag tatcatctaa
      361 taaataatcg aaatttgagt ttaaatgttt ttttataaat gaaactctat ccatcttttg
      421 atgttcattt aaatcgctta aacttcttaa taacaacaaa tcttcataaa cctcaactgt
      481 tacatcaaat ttcaaataat caactaagtt tccatactta tcaataattt cttctagcgt
      541 aggtatcaat atataccccc cctccccatt ttttgttccg cgtctaatca taatgcttca
      601 tttatgctta ttatagcaat caattgaaga tagtttaacc tatatgatat tgattgactc
      661 cttaaaaatt attggaggaa ttttaaaatg aataagaaat ctaagcagca agaaaagctt
      721 tataatttta taattgctaa atcttttcaa caaccagttg gaagtacgtt cacttatggt
      781 gaattaagaa agaaatataa tgtggtttgt agcacgaatg atcaacgtga agtaggacgt
      841 cgttttgctt actggattaa gtacacgcca ggattaccat ttaaaattgt aggaacaaaa
      901 aatggcagtt tattatatca gaaaataggg ataaacccat gcaataatag cacgccttcg
      961 aaaggaggtg attgctaatg agttacacat tgtttgaaat tggcccattt gctttagatt
     1021 ccttcggttg gaaagaaacg ataccaccta aaaaagatgg tgacagtgaa aaagtggtta
     1081 gaatggctag ccctattatt gttaatgcaa gattttctga tccaattact ggtgttgaaa
     1141 agttgattat tactaacaac aatggtaaaa aagacatttt tgagtctgat atcttaacaa
     1201 cacgaaactt accttcttta attaaatatg gctacagtat taacgagaag tatattagat
     1261 cattgagtta tgccttgcaa ttgatgcgag atagattacc actttctgaa ttatatgagg
     1321 gtgtaggtat actagagaca ccttttggct acttgatatc gctagataaa gttctcaaat
     1381 ctattcaatt taatcagtcc tcaccttcat accctattgt agatagtgct tatgacttaa
     1441 ctcctaaagg tacatttgat aattggttta acatgtatat tgacgaggtt aaaggtcatc
     1501 ttcttttaga attggcagtc atatttggga tttctgcctt agtgacaagt tttctgaagc
     1561 ataaacatga aatcgaattt gcaggaattc tctttagttt tacaggtcaa tcctcaaccg
     1621 gtaagtcaac cgcagctgct ttagctgtat cagtcgctgg taaccctacc aaaggtaatg
     1681 aaactctttt tagaagttgg aatgccacac gaaacgcact tgaaggttat ttgagtaaca
     1741 attatggcat tcctatagta tttgatgagc tttcatctac gacacttaga gataccacgg
     1801 gtttgttata ttccatcgct gaaggtcaag gtcgtcaacg ttcaaacgtt catggagaag
     1861 ttaaaacgcc taagaattgg ggtacttctg tcatttcaac gtcagaatac agtattttta
     1921 atgattcagc tcaaaacgat ggattacgtg taagaaccat cgagattaat gaacaattca
     1981 ctacaaatgc gaccaatgct gacaatatta aaaaagctgt agcactaaac tatggtcatg
     2041 ttttaccgtt agtagctaaa tatcttataa atcgtgaaga tgaagttatt caatggtttt
     2101 acaaggaagt agattggttt gaagctaaat tgaaagatga aacaaacaat acaggtattc
     2161 gtatgtttaa gcgttatgca gtcattacaa catctgcgaa aatattaggt cgtgttttat
     2221 ctacagatat tgatatcgct aatataagag actactttat tgattatcat actcatacag
     2281 tctctgaacg ctcattagct gataaagcta ttgatgtcat cattcaattt gtggcacaaa
     2341 acagaggtaa gttttcagat gaaggtgctt tgaaaaatat gtttgagaac tatggattaa
     2401 tctcattaaa agatgaccat atcgaagtaa aaataattgc gaacgttttt aaacagatgc
     2461 taaacaatca tcaatttcaa gatgttaata atgttgtcaa cgccttaaga gataaaggtt
     2521 tcatattagc tgaccgtgga cgccaaacaa ctaaaagaag tgtcaaagat aatagcggta
     2581 aaaaacaatc acttgtattt taccatctaa aattagatgt agagtttgca tctatcctcg
     2641 gattaactaa agataagtcg ttacttcaaa attggacacc gtcaaatgat aacaaagctg
     2701 cgaaagaact ttttaaatcc gctaatgaag ggataggccc ttcaggagtt cacgaagatt
     2761 tctaattgtc tttatcatac aactgtgaag catcaaactg aataatcgac gtattgaaga
     2821 tagaacaaat aacggagtga aaaacatgga tgaacttatc gagatattag ccgatttggt
     2881 aattgaagaa atcaatcaaa caaatgacta atatggacgc ccaacatctc attgatgttg
     2941 ggcttatata tcaattcatc cataaatata tattatcact gtgaaaggag gagccgatat
     3001 gaaacaagtc ataggctatt tacgtcaaag tacgatgaaa caacaatctc ttgcagcaca
     3061 gaaacaagct atcgaagcaa tagccgaaaa acatcatatt caacatatca acttttatag
     3121 cgacaaacaa tcaggtcgca aagataatcg tagtgggtat cgacaaataa cacaattaat
     3181 tcaacaagga cagtgtgaca tattatgctg ttatcgtctt aataggttgc atcgtaatct
     3241 gaaaaatgca ttaaaactca tcaaattatg tcaaacgtac catgttcata tcttaagcgt
     3301 acatgatggt tactttgata tggatcaagc tttcgaccga ctcaagctta atatcttcat
     3361 cagtttagcc gaacttgaat cggataacat tggagaacaa gtcagaaatg ggcttcaaga
     3421 aaaagcaaag caaggtcgat tgattacaac acatgcacct tttggttacg aatatcacaa
     3481 cggaacattc atcatcaatc aaaatgagtc accaacggta aaggctgtat tcaattatta
     3541 cattaaaggt catggttata agaaaattgc acagttatta gaagaagata acacgtatat
     3601 caatcgacaa ccctatcaag ttcgtaacat tatcatcaat cctaattatt gtggtcgtgt
     3661 caacaatcaa tatggccaat tcgacaatat gtttccttct attgtttcca caagtatata
     3721 tgagcaagcg cagagacttc gattgcaaaa acaaaccaaa cagacacctt cggataatca
     3781 actcaaacaa aaaatcaaat gcccatgttg taatgcaaca cttacaaata tgaccattag
     3841 aaaaaagaat catacattac gttactacgt ctgtcctaaa aacatgaatg cttcacgctt
     3901 tgtctgtgat tttaaaggca tcaatgcaca aacacttgaa gataaagtat tagaagtgtg
     3961 ccgagacttt tatcaaaatc aacgcatcta cacaaaaatt aaaagtgcga ttgacaaacg
     4021 catcaaaaga caaagaaaca tagaaaaaca tcacacattg actcaagaac aactgataga
     4081 aaagttggca caaggcatca ttgatgcaga aacgttcaga gagcaaacac aatcattacg
     4141 tcaacaaccg caacgcacta catctatcaa tgggcatcaa atacaacaca tcattcaaaa
     4201 tattattcaa aaacgtttca cgttaaacat attgtacccc tatattgaaa ccattcacat
     4261 tacgaaagat aaaaatctta taggaatcta tttcaaaaat gaaccactca atattgtcaa
     4321 tcaaaccatg caatcatcga ttgcataaat gaagaaagga tgtaacataa tgcaacaact
     4381 taaaacaaaa cgtgtcggta tctatgtacg tgtatcaaca gaaatgcaaa gcacagaagg
     4441 ttatagtatc gacggacaaa tcaatcaaat caaagaatac tgtgacttcc atcattttga
     4501 agttaaagat atatacgctg accgtggtat ttcaggtaaa tctatgaaca gacctgagct
     4561 ccaacgtatg ttgaaagatg caaaagaagg caatatcgac tgtgttatgg tatacaaaac
     4621 aaaccgatta gctcgtaata catctgatct tctcaaaatt gtcgaagatt tacacaaaca
     4681 aaatgtcgaa tttttcagtt tgtcagagcg tatggaagtc aatacttctt ctggtaaact
     4741 catgttacag atacttgcga gtttctcaga attcgaacgt aataacattg tcgagaacgt
     4801 atttatgggt caaacgagac gtgcccaaga aggctattat caaggcaatt taccactagg
     4861 ttatgacaaa ataccagata gtaaacacga gctaatgatt aaccaacatg aagctaatat
     4921 tgtaaaatat atattcgagt gctatgccaa aggacatggc tatcgtaaaa ttgccaatgc
     4981 attgaatcac aaaggatatg tcactaaaaa ggggaaacct ttcagtatta gttcaatcac
     5041 atacatctta gctaaccctt tctatatcgg caaaattcaa tttgcgaaat acaaagattg
     5101 gagtgaaaaa cgtcgtaaag ggctgaatga taaaccagtg atagctgaag gtaagcattc
     5161 ccccattatt aatcaagatt tatgggataa agtacaaatg cgtaagaaac aagtcagtca
     5221 aaaaccccaa gtccatggca aaggaacgaa tctgcttaca ggcattattc actgtcccca
     5281 atgtggcgca cctatggcag caagcaatac cacgaatact cttaaagacg ggactaagaa
     5341 acgtattcgt tactattcat gtagtaattt tcggaacaag ggttccaaag tatgttcggc
     5401 aaacagtgta agagctgatg tgattgaaga ttatgtgatg aagcaaatac ttgaaatagt
     5461 caaaagtgat aaagtcattc aacgcgtagt aacacacgtc aatcaagaaa atcaagttga
     5521 tggcgctgca cttcatcacg atattgctta taagcaacaa caatatgatg aagtacaaat
     5581 caaaataaat aacttgatta aaaccatcga ggataatccg gacttaacat cagtaatcag
     5641 accaagtatt caaaaatatg aaaagcaact caatgacatt acgaatcaaa tcaaccaact
     5701 caaaaatcaa caaaatgaag ataagacttt atttgatgcc aaagaaatca gtaaactatt
     5761 acaacacatc tttcatgata ttaagcacat agaaaaatct cgactcaaag cattgtatct
     5821 atcagtgatt gatcgcattg atattaaaaa agatggtaat cataaaaaac aattctatgt
     5881 cacactcaaa cttaataacg aaatcattaa acaacttttc aataataaac aactcgacga
     5941 agtgcatctc agcacttcgt ctttattttt gccccaaaca ctatatctta ctatttagct
     6001 aatagaatca ttgttataac aataattttc actatattct ttcgcttatt ttatctaact
     6061 attttcaaga attgctcttt atcaatttca ataacactta tctaatgaaa cagttattgt
     6121 cgtaattttt tcataactat acaatgctta tattgaaatt gctaaagaac atcccacaat
     6181 gattttttat cgggaaatga ttgggatttg cagattcgaa gaaacagaag gattgtgcaa
     6241 gcacaatccc aaaatcccgt tactttttat aaataaataa tatatataag gaagcagaga
     6301 cacaacccta tcatcaatac ttataaataa tgagttgatt gtttgtcaaa ataggcggaa
     6361 tttcaggatt tccttggcat accaacgttt gtagcgggtt tttatatgga attttctctt
     6421 attcccaccg ggattcgtaa taaatgacaa atcaaatata tattatctca atgatgtata
     6481 tgaactacat cactattaca ctatctagga ggcaataata tgaaaaccat cactcaagaa
     6541 ctcaaacaat atataaccca cctattccaa ctatctaata acgaagcttg ggaatgtgaa
     6601 gcgctggagg aagcagcaga aaatattcta ccggaacgtt ttattaatga ttctccactg
     6661 gtacatctca cactcgagac ttatacctac tataatgatg aactacatga gctaagtatc
     6721 tatccatttc taatgtatgc caataaccaa ctcatcagtg tcggatactt agaccatttt
     6781 gacatggact ttctgtacct tacagatact caaaatatca ttattgatga acgtcattta
     6841 ctaaaacaag gaggtcagga ccatgaataa ttggatcata gttgctcaat tatccgtcac
     6901 agttatcaac gaaatcatcg acatcatgaa agaaaagcag aagggaggga aatagcatga
     6961 aaatcaatcg atacatcaca agaggaatta atgaaagtat tccactagac cttcaaatct
     7021 tactttggca catggtagaa aaaaaagata accagcctca tacagattac ctacacattt
     7081 ttaaattaca agaagatgag aatatactct caatcacaca tgaacaagaa caacccacat
     7141 acaaattgga atatcactat acaaactatg aaaaaaatca aaatgcatta cctaagaaag
     7201 tctacgtcat tcgtgaagat gacgtggacg ctttttatta tgtgatgctt ttaccagaag
     7261 aatactaaag gaagtgacga tgatgaatac aatcaaaagt acgatacaca cagaagccat
     7321 atttagcgat gatgaacagc accgctattt actcaagaaa acatgggatg aaaagaaagc
     7381 tgcatgcaca gtgataacga tgtatcctca tttagacggt gtattatcac tcgatctcac
     7441 aactgttctt atcctcaacc aattagccaa ttcagaacga tacggtgctg tatatcttgt
     7501 aaatctattc tctaatatta gaacacccga aaacctcaaa catatcaaaa atccatacga
     7561 tgagcacact gatattcatt tgatgaaagc gattagtgaa agtgacacag tgattcttgc
     7621 ttatggtgcc tatgcgaagc gaccagttgt tatcgaccgt gtcgaacaag tgatggaaat
     7681 gttaaaacct cataaaaaga aagtaaaaaa gctcatcaat ccagtaacaa atgaaattat
     7741 gcatccactc aaccctaagg cacgtcaaaa atggattttg aaatcatagc aaatacatgg
     7801 aggtcaatat ggaaacaaaa caaatcaaca tcgttttact gtaaatgata ttgttgaaac
     7861 aattcctgac aatgaattcg acgaattcag acatcaccgt ggtgcaactt cgtaccatcc
     7921 taaaatgatg ttaaaagtga ttctatatgc ctacacacaa tctgtattct caggtcgtaa
     7981 aatagaaaaa atgcttaatg atagcatccg aatgatgtgg ctatcacaaa atcaaaaacc
     8041 ttcttataaa acaattaatc aatttagagt aaatccaaaa gtagatgctt tattagaatc
     8101 tttatttatt caattttaca gtcagtgtgt aaaacaaaat cttatagatg ataaagctat
     8161 ttttattgat ggtacaaaaa ttgaagcaaa tgccaatcga tatacatttg tatggaaaaa
     8221 gagtattcaa aaccatgaat caaagatgaa tgaggattct aaagccctct accatgaatt
     8281 ggtaaccaat aaaatcatac cggaaattaa agaagatcat gataatgaat taacaaaaga
     8341 agaaatagat ttgattggta gtcacttaga taaagaaatc gaagatttaa accaacatat
     8401 caacaatgaa aaatgtacta aaacaagaaa acaaatacgt ctcaaaagaa ctaaaatcaa
     8461 aaaatacaaa aagcaaatca atgattattt tgagcgaaag tatcgatacg aatttcaaaa
     8521 atctatttta aaggatagaa atagttattc taagacagat tatgatgcga catttatgag
     8581 aatgaaagaa gatcacatga aaaatggaca acttaagcca gggtataatt tacaaatagc
     8641 aacaaattcc caatttgttt tatcttataa tgtgtatcaa aatccaacgg atactagaac
     8701 gatgattcca tttttaaatt caattcaaga gacctacggt catttacctg aatatattgt
     8761 agctgatgca ggttatggta gtgaatcaaa ttataaggca attatagatg actttaatcg
     8821 aacgccactc ataacatatg gaatgtttat aaaagataaa actaaaaaat ataaaagtga
     8881 catctttaat actcaaaatt ggaactatga cgaaattaat gacgaattca tttgtccgaa
     8941 taataaacgg ctaggtttta aaagatatgc ctatcgtcat gataagtatg gttataagcg
     9001 agacttcaaa ttatatgaat gtgatgattg ttcagaatgt cctctgaaaa atcaatgtat
     9061 gaacttcaat tcaaaaacaa acaaaaaaat aatgaagaat tataactggg aatattttaa
     9121 atcccaaatt aacaaaaagc tttcagaacc agaaacaaaa aatatctaca gtcaaagaaa
     9181 aattgatgtg gaacctgttt ttggatttat gaaggctatt ttgggtttca ctcggatgtc
     9241 tgtccgagga ctcaataaag tcaaaagaga acttggtttt gtattaatgg cacttaatat
     9301 aagaaaagta gtagctcaac gagctgaaaa taatcaaaaa atttataaaa aagacaattt
     9361 ctatattatt tcaatagaaa ttgtcttttt tcacttatcc aagaacttta tgtcccggac
     9421 tcattatgat ttattttttt attgctgact tgccattaat tctgcttgct ctacaacttg
     9481 ttccacagcc attttttgaa gatctggtgg atagccatat ttctttaata ggcgtctaac
     9541 ttgaactctc attttagctt tagcactgtc tcgtttagac caatcaacac tcatgttatc
     9601 cttaactgtt ttagttaact catgtgcaat ggctcttaac tctttatcgc ccaaagcttc
     9661 tttagctgtt tcatgtgaag ctcgatatac tacatacaat atacgcaaca atacgcttgt
     9721 ttcgattagg tttaggcatt gaatcataaa gtgcaatata cttaacacgt tctttaatat
     9781 ttgaattaaa acctagtaaa tattgtgctg ccacattatt tatgtgctga gattttaaaa
     9841 tagagcattt taatatcgat tcaccataac gtatatgttc atggcgattc aaaattttta
     9901 aaacgtttct atcacatact ttttcacagt cattgtccat cattgtttta cttatatata
     9961 gtgcaggatt aaaccagaat atcattttaa aaacaacata aagctggttg aatattaagt
    10021 catgactttt cacatgtgat agttcatgta gaataatata ttcaatttct ttgtcattca
    10081 tggtttcgac tacgacagtt ggtagtacaa tttgggattt cactaaacca aataccatcg
    10141 gattatcaat gtttgaacta taactaattg ttatatgctt tttgtagaac tgcatcttac
    10201 tttgacatac tttaagtcgt tcattaagat atgacgattc caatgacgaa cttttaataa
    10261 catcaatttg tcggaatgcc ttaatcatat aaaataagca caacaaacta ccaaataccc
    10321 atatcaaaag aatcatatac gttatatttg aggtctcaaa ctgattaaca ttaattgcta
    10381 agtctttcgt aacagatgat tgttgaccat ctaacatatg actaaccgaa gaagtcgtgt
    10441 cagatacatt tcgattcatc atatcttttg aaaatgtaaa attcgatatt ttgtaaaatg
    10501 gtattaatgg aattaacgtg gagacgagca ctaataacca aatcttatgt gacataatat
    10561 tttgagtata ttttatatag agcattctca ctaaaaaaat tacacatatc gtgagcaatg
    10621 aactgattat acttaacatt aaaaaagatg ataacacctt ctacacctcc atatcacaaa
    10681 aattataaca ttattttgac ataaatacta catttgtaat atactacaaa tgtagtctta
    10741 tataaggagt atattgatga aaaagataaa aattgttcca cttattttaa tagttgtagt
    10801 tgtcgggttt ggtatatatt tttatgcttc aaaagataaa gaaattaata atactattga
    10861 tgcaattgaa gataaaaatt tcaaacaagt ttataaagat agcagttata tttctaaaag
    10921 cgataatggt gaagtagaaa tgactgaacg tccgataaaa atatataata gtttaggcgt
    10981 taaagatata aacattcagg atcgtaaaat aaaaaaagta tctaaaaata aaaaacgagt
    11041 agatgctcaa tataaaatta aaacaaacta cggtaacatt gatcgcaacg ttcaatttaa
    11101 ttttgttaaa gaagatggta tgtggaagtt agattgggat catagcgtca ttattccagg
    11161 aatgcagaaa gaccaaagca tacatattga aaatttaaaa tcagaacgtg gtaaaatttt
    11221 agaccgaaac aatgtggaat tggccaatac aggaacagca tatgagatag gcatcgttcc
    11281 aaagaatgta tctaaaaaag attataaagc aatcgctaaa gaactaagta tttctgaaga
    11341 ctatatcaaa caacaaatgg atcaaaattg ggtacaagat gataccttcg ttccacttaa
    11401 aaccgttaaa aaaatggatg aatatttaag tgatttcgca aaaaaatttc atcttacaac
    11461 taatgaaaca gaaagtcgta actatcctct aggaaaagcg acttcacatc tattaggtta
    11521 tgttggtccc attaactctg aagaattaaa acaaaaagaa tataaaggct ataaagatga
    11581 tgcagttatt ggtaaaaagg gactcgaaaa actttacgat aaaaagctcc aacatgaaga
    11641 tggctatcgt gtcacaatcg ttgacgataa tagcaataca atcgcacata cattaataga
    11701 gaaaaagaaa aaagatggca aagatattca actaactatt gatgctaaag ttcaaaagag
    11761 tatttataac aacatgaaaa atgattatgg ctcaggtact gctatccacc ctcaaacagg
    11821 tgaattatta gcacttgtaa gcacaccttc atatgacgtc tatccattta tgtatggcat
    11881 gagtaacgaa gaatataata aattaaccga agataaaaaa gaacctctgc tcaacaagtt
    11941 ccagattaca acttcaccag gttcaactca aaaaatatta acagcaatga ttgggttaaa
    12001 taacaaaaca ttagacgata aaacaagtta taaaatcgat ggtaaaggtt ggcaaaaaga
    12061 taaatcttgg ggtggttaca acgttacaag atatgaagtg gtaaatggta atatcgactt
    12121 aaaacaagca atagaatcat cagataacat tttctttgct agagtagcac tcgaattagg
    12181 cagtaagaaa tttgaaaaag gcatgaaaaa actaggtgtt ggtgaagata taccaagtga
    12241 ttatccattt tataatgctc aaatttcaaa caaaaattta gataatgaaa tattattagc
    12301 tgattcaggt tacggacaag gtgaaatact gattaaccca gtacagatcc tttcaatcta
    12361 tagcgcatta gaaaataatg gcaatattaa cgcacctcac ttattaaaag acacgaaaaa
    12421 caaagtttgg aagaaaaata ttatttccaa agaaaatatc aatctattaa ctgatggtat
    12481 gcaacaagtc gtaaataaaa cacataaaga agatatttat agatcttatg caaacttaat
    12541 tggcaaatcc ggtactgcag aactcaaaat gaaacaagga gaaactggca gacaaattgg
    12601 gtggtttata tcatatgata aagataatcc aaacatgatg atggctatta atgttaaaga
    12661 tgtacaagat aaaggaatgg ctagctacaa tgccaaaatc tcaggtaaag tgtatgatga
    12721 gctatatgag aacggtaata aaaaatacga tatagatgaa taacaaaaca gtgaagcaat
    12781 ccgtaacgat ggttgcttca ctgttttatt atgaattatt aataagtgct gttacttctc
    12841 ccttaaatac aatttcttca ttttcattgt atgttgaaag tgacactgta acgagtccat
    12901 tttctttttt tatggatttc ttatttgtaa tttcagcgat aacgtacaat gtattacctg
    12961 ggtatacagg tttaataaat ttaacgttat tcatttgtgt tcctgctaca acttcttctc
    13021 cgtatttacc ttcttctacc cataatttaa atgatattga aagtgtatgc atgccagatg
    13081 caatgatacc tttaaatcta ctttgttctg ctttttcttt atctatatgc atatattgag
    13141 gatcaaaagt tgttgcaaat tggataattt cttcttctgt aatatgaagg ctttttgttt
    13201 tgaatgtttc tcctactata aaatcatcgt atttcatata tgtctctctt tcttattcaa
    13261 attaattttt tagtatgtaa catgttaaag gtaagtctac cgtcactgaa acgtaagact
    13321 cacctctaac tttctattga gacaaatgca ccattttatc tgcattgtct gtaaagatac
    13381 catcaactcc ccaattagca agttggtttg cacgtgctgg tttgtttaca gtccatacgt
    13441 tcaattcata acccgcttct tttaccattt ttacttttgc tttagtaagt ttggcatctt
    13501 cagtgtttac tattttagca ttacagtaat ctaaaagtgt tctccagtct tcacgaaacg
    13561 aagttgtatg gaatataact gctctgttat attgtggcat gatttcttct gcaagtttaa
    13621 caagcacaac attaaagctt gaaatgagca cttcttgatt ctgatttaag tttgttaatt
    13681 gttcttccac ttgcttaacc atacttttag aaagtgctag tccattcggt ccagtaatac
    13741 cttttaattc tacatttaaa ttcatattat attcatttgc tatttttact acatcatcga
    13801 aagttggcaa atgttcatct ttgaattttt caccaaacca agatcctgca gaagcatctt
    13861 taatttcatc ataattcaat tcagttattt ccccggacat atttgtagtc cgttctaaat
    13921 aatcatcatg aatgataatc agttgttcat cttttgtaat tgcaacatct aactccaacc
    13981 agtttatacc ttctacttct gaagcagctt taaatgatgc aattgtattt tccggagctt
    14041 tactaggtaa tcctctatgt ccatatacag ttagcatatt acctctcctt gcatttttat
    14101 ttttttaatt aacgtaactg tattatcaca ttaatcgcac ttttatttcc attaaaaaga
    14161 gatgaatatc ataaataaag aagtcgatag attcgtattg attatggagt taatctacgt
    14221 ctcatctcat ttttaaaaaa tcatttatgt cccaagctcc attttgtaat caagtctagt
    14281 ttttctgtac cccttatctg caattttact taggattgct tttaacttac cccttatcag
    14341 caattttact gagaactgct tttaacgcac ctcttatctg caattttgcc tagaactgct
    14401 tttaacgtac ctcttatctg caattttact gagaactgct tttaacttac cccttatcag
    14461 caattttgca tggaattgct tttaacgtac ctcttatctg caattttact tagaactgct
    14521 tttaacaaac ctcttatctg caattttact tagaactgct tttaacgtac ctcttatctg
    14581 taaatttact gagaactgct tttaacaaac ctcttatctg caattttact tagaattgct
    14641 tttactattc ctcttattag tataatctca gtaagaatgc gtataaaaat gaaaattaca
    14701 accgattttg taagtgctga cgcctgaggg aatagtatgt gcgagagact aatggctcga
    14761 gccatacccc taggcaagca tgcacgtaca aaatcgtaag ataaaaaaat aagcatatca
    14821 ctgtaaactt taaaaaatca gtttagtgat atgcttattt atttcgagtt aggatttatg
    14881 tcccaagctc atcaagcaca atcggccact agtttatttc tctatcttat atgttctgat
    14941 atggtcttct atactgtata agtatacttt tgaatatgga tcttgtgtca attcacgttc
    15001 gaaatcaaat tcttgattat caaatctgtt aaagaatgtt tcgtattctt cgactgataa
    15061 ttgctctcta gattctagca tatttaagtg tttctcttta tctaatgctt tgtcatatcc
    15121 tttaacgatt gaaccactaa agatttctcc tactgctcct gaaccataac taaatagaca
    15181 tactttctct tctggttgga atgtgtggtt ctgtaataac gaaattaaac ttaagtataa
    15241 tgatcctgta taaatgttac caacatctct attccataat acggttctgt tgcaaagttg
    15301 aatttatagt ataattttaa caaaaaggag tcttctgtat gaactatttc agatataaac
    15361 aatttaacaa ggatgttatc actgtagccg ttggctacta tctaagatat acattgagtt
    15421 atcgtgatat atctgaaata ttaagggaac gtggtgt
//
LOCUS       DAKTIO010000051        15201 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.051, whole genome shotgun sequence.
ACCESSION   DAKTIO010000051 DAKTIO010000000
VERSION     DAKTIO010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15201
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.051"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(362..435)
                     /locus_tag="O0293_001204"
     tRNA            complement(362..435)
                     /locus_tag="O0293_001204"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(399..401),aa:Arg,seq:tct)
     gene            799..933
                     /locus_tag="O0293_001205"
     CDS             799..933
                     /locus_tag="O0293_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HCY0280094.1"
                     /translation="MEGLFNAIKDTVTAAINNDGAKLGTSIVSIVENGVGLLGKLFGF
                     "
     gene            990..1124
                     /locus_tag="O0293_001206"
     CDS             990..1124
                     /locus_tag="O0293_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HCY0280095.1"
                     /translation="MTGLAEAIANTVQAAQQHDSVKLGTSIVDIVANGVGLLGKLFGF
                     "
     gene            1248..1943
                     /locus_tag="O0293_001207"
     CDS             1248..1943
                     /locus_tag="O0293_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HCY0280096.1"
                     /translation="MGKLGYKNILIDFDDTIVDFYDAEEWAFHYMANVFNHKATKDDF
                     LTFKKINHQHWEAFQQNKLTKSEVLSERFVNYFKHHQMEVDGHRADVLFRNGLAEAKV
                     KYFDQTLETIVELSKRHDLYIVTNGVTETQKRRLNQTPLHKYIKKIFISEETGYQKPN
                     PEFFNYVFNDIGEDERQHSIIVGDSLTSDILGGINAGIATCWFNFRGFDHNPGIIPDY
                     EINSWKQLNDIVR"
     gene            complement(2052..2492)
                     /locus_tag="O0293_001208"
     CDS             complement(2052..2492)
                     /locus_tag="O0293_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCY0280097.1"
                     /translation="MSEIKRLEINYKTDELFENFRAFGNKDLYMVNELNGQMIDASSD
                     SPFYGIFVGDQLGARMALLKKGDVEEIYFPDFEDYILLWKLEVLPKYQNRGYASELID
                     FAKSFNMPIKAIGRNDSKDFFLHHGFTDVEAKNIEGHDVLLWKP"
     gene            2687..4300
                     /gene="bshC"
                     /locus_tag="O0293_001209"
     CDS             2687..4300
                     /gene="bshC"
                     /locus_tag="O0293_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HCY0280098.1"
                     /translation="MDCKVVSLNEKDQFIPKIKSSDPVITGLFQYDAAQQTSFEKRMS
                     KENNGREAALANVIREYMSDLKLSSEQELNIQHLANGSKVVIGGQQAGLFGGPLYTFH
                     KIFSIITLSKELTDTHKQQVVPVFWIAGEDHDFDEVNHTFVYNENHGSLHKVKYHTME
                     MPETTVSRYYPDKAELKQTLKTMFIHMKETVHTQGLLEICDRIIDQYDSWTDMFKALL
                     HETFKAYGVLFIDAQFEPLRKMEAPMFKKILKKHQLLDDAFRATQQRTQNQGLNAMIQ
                     TDTNVHLFLHDENMRQLVSYDGKHFKLNKTDKTYIKEEIINIAENQPELFSNNVVTRP
                     LMEEWLFNTVAFVGGPSEIKYWAELKDVFELFDVEMPIVMPRLRITYLNDRIEKLLSK
                     YNIPLEKVLVDGVEGERSKFIREQASHQFIEKVEGMIEQQRRLNKDLLDEVAGNQNNI
                     NLVNKNNEIHIQQYDYLLKRYLLNIERENDISMKQFREIQETLHPMGGLQERIWNPLQ
                     ILNDFGTDVFKPSTYPPLSYTFDHIIIKP"
     gene            4444..4875
                     /gene="mraZ"
                     /locus_tag="O0293_001210"
     CDS             4444..4875
                     /gene="mraZ"
                     /locus_tag="O0293_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCY0280099.1"
                     /translation="MFMGEYDHQLDTKGRMIIPSKFRYDLNERFIITRGLDKCLFGYT
                     LDEWQQIEEKMKTLPMTKKDARKFMRMFFSGAVEVELDKQGRINIPQNLRKYANLTKE
                     CTVIGVSNRIEIWDRETWNDFYEESEESFEDIAEDLIDFDF"
     gene            4891..5826
                     /gene="rsmH"
                     /locus_tag="O0293_001211"
     CDS             4891..5826
                     /gene="rsmH"
                     /locus_tag="O0293_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCY0280100.1"
                     /translation="MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLND
                     DGRLIAIDQDQTAIDNAKEVLKDHLHKVTFVHSNFRELTQILKDLNIEKVDGIYYDLG
                     VSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFS
                     KQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELS
                     AFEDSIEQAIELVKVDGRISVITFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTP
                     KLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK"
     gene            5840..6241
                     /gene="ftsL"
                     /locus_tag="O0293_001212"
     CDS             5840..6241
                     /gene="ftsL"
                     /locus_tag="O0293_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCY0280101.1"
                     /translation="MAVEKVYQPYDEQVYNSIPKQQPQTKPEKKTVSRKVVVQLTKFE
                     KVLYITLITVIAMLSIYMLSLKMDAYDTRGKIADLDYKIDKQSSENSALQSEIKKNSS
                     YERIYEKAKKQGMSLENDNVKVVRSNGEAKN"
     gene            6222..8456
                     /locus_tag="O0293_001213"
     CDS             6222..8456
                     /locus_tag="O0293_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCY0280102.1"
                     /translation="MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNG
                     QDLVMKANEKYLVKNAQQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRH
                     VVDKKETAKKLSTVINMKPEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPG
                     ISLLPETERFYPNGNFASHLIGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIH
                     DIWGYIAPNTKKEKQPKRGDDVHLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAK
                     TGEILAYSQRPTFNPETGKDFGKKWANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDK
                     KYKSGHRDIMGSRISDWNRVGWGEIPMSLGFTYSSNTLMMHLQDLVGADKMKSWYERF
                     GFGKSTKGMFDGEAPGQIGWSNELQQKTSSFGQSTTVTPVQMLQAQSAFFNDGNMLKP
                     WFVNSVENPVSKRQFYKGQKQIAGKPITKDTAEKVEKQLDLVVNSKKSHAANYRIDGY
                     EVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMGDAPKKNPKVIVYAGMSLAQKNDQEAY
                     ELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYSKVPDVEGQDKQKAIDNVSAKSLEP
                     VTIGSGTQIKAQSIKAGNKVLPHSKVLLLTDGDLTMPDMSGWTKEDVIAFENLTNIKV
                     NLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNSTNNSDSNSDDKKKSDSKTDK
                     DKSD"
     gene            8748..9713
                     /locus_tag="O0293_001214"
     CDS             8748..9713
                     /locus_tag="O0293_001214"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCY0280103.1"
                     /translation="MIFVYALLALVITFVLVPVLIPTLKRMKFGQSIREEGPQSHMKK
                     TGTPTMGGLTFLLSIVITSLVAIIFVDQANPIILLLFVTIGFGLIGFIDDYIIVVKKN
                     NQGLTSKQKFLAQIGIAIIFFVLSNVFHLVNFSTSIHIPFTNVAIPLSFAYVIFIVFW
                     QVGFSNAVNLTDGLDGLATGLSIIGFTMYAIMSFVLGETAIGIFCIIMLFALLGFLPY
                     NINPAKVFMGDTGSLALGGIFATISIMLNQELSLIFIGLVFVIETLSVMLQVASFKLT
                     GKRIFKMSPIHHHFELIGWSEWKVVTVFWAVGLISGLIGLWIGVH"
     gene            9715..11064
                     /locus_tag="O0293_001215"
     CDS             9715..11064
                     /locus_tag="O0293_001215"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCY0280104.1"
                     /translation="MLNYTGLENKNVLVVGLAKSGYEAAKLLSKLGANVTVNDGKDLS
                     QDAHAKDLESMGISVVSGSHPLTLLDNNPIIVKNPGIPYTVSIIDEAVKRGLKILTEV
                     ELSYLISEAPIIAVTGTNGKTTVTSLIGDMFKKSRLTGRLSGNIGYVASKVAQEVKPT
                     DYLVTELSSFQLLGIEKYKPHIAIITNIYSAHLDYHENLENYQNAKKQIYKNQTEEDY
                     LICNYHQRQVIESEELKAKTLYFSTQQEVDGIYIKDGFIVYKGVRIINTEDLVLPGEH
                     NLENILAAVLACILAGVPIKAIIDSLTTFSGIEHRLQYVGTNRTNKYYNDSKATNTLA
                     TQFALNSFNQPIIWLCGGLDRGNEFDELIPYMENVRAMVVFGQTKAKFAKLGNSQGKS
                     VIEANNVEDAVDKVQDIIEPNDVVLLSPACASWDQYSTFEERGEKFIERFRAHLPSY"
     gene            11080..12399
                     /locus_tag="O0293_001216"
     CDS             11080..12399
                     /locus_tag="O0293_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HCY0280105.1"
                     /translation="MDDKTKNDQQESNEDKDELELFTRNTSKKRRQRKRSKATHFSNQ
                     NKDDTSQQADFDEEIYLINKDFKKEESNDKNNDSASSHANDNNIDDSTDSNIENEDYR
                     YNQEIDDQNESNVISVDNEQPQSAPKEQNSDSIDEETVTKKERKSKVTQLKPLTLEEK
                     RKLRRKRQKRIQYSVITILVLLIAVILIYMFSPLSKIAHVNINGNNHVSTSKINKVLG
                     VKNDSRMYTFSKKNAINDLEENPLIKSVEIHKQLPNTLNVDITENEIIALVKYKGKYL
                     PLLENGKLLKGSNDVKINDAPVMDGFKGTKEDDMIKALSEMTPEVRRYIAEVTYAPSK
                     NKQSRIELFTTDGLQVIGDISTISKKMKYYPQMSQSLSRDSSGKLKTRGYIDLSVGAS
                     FIPYRGNTSSQSESDKNVTKSSQEENQAKEELQSVLNKINKQSSKNN"
     gene            12505..13917
                     /gene="ftsA"
                     /locus_tag="O0293_001217"
     CDS             12505..13917
                     /gene="ftsA"
                     /locus_tag="O0293_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCY0280106.1"
                     /translation="MEEHYYVSIDIGSSSVKTIVGEKFHNGINVIGTGQTYTSGIKNG
                     LIDDFDIARQAIKDTIKKASIASGVDIKEVFLKLPIIGTEVYDESNEIDFYEDTEING
                     SHIEKVLEGIREKNDVQETEVINVFPIRFIVDKENEVSDPKELIARHSLKVEAGVIAI
                     QKSILINMIKCVEACGVDVLDVYSDAYNYGSILTATEKELGACVIDIGEDVTQVAFYE
                     RGELVDADSIEMAGRDITDDIAQGLNTSYETAEKVKHQYGHAFYDSASDQDIFTVEQV
                     DSDETVQYTQKDLSDFIEARVEEIFFEVFDVLQDLGLTKVNGGFIVTGGSANLLGVKE
                     LLSDMVSEKVRIHTPSQMGIRKPEFSSAISTISSSIAFDELLDYVTINYHDNEETEED
                     VIDVKDKDNESKLGGFDWFKRKTNKKDTHENEVESTDEEIYQSEDNHQEHKQNHEHVQ
                     DKDKDKEESKFKKLMKSLFE"
     gene            13950..15122
                     /gene="ftsZ"
                     /locus_tag="O0293_001218"
     CDS             13950..15122
                     /gene="ftsZ"
                     /locus_tag="O0293_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCY0280107.1"
                     /translation="MLEFEQGFNHLATLKVIGVGGGGNNAVNRMIDHGMNNVEFIAIN
                     TDGQALNLSKAESKIQIGEKLTRGLGAGANPEIGKKAAEESREQIEDAIQGADMVFVT
                     SGMGGGTGTGAAPVVAKIAKEMGALTVGVVTRPFSFEGRKRQTQAAAGVEAMKAAVDT
                     LIVIPNDRLLDIVDKSTPMMEAFKEADNVLRQGVQGISDLIAVSGEVNLDFADVKTIM
                     SNQGSALMGIGVSSGENRAVEAAKKAISSPLLETSIVGAQGVLMNITGGESLSLFEAQ
                     EAADIVQDAADEDVNMIFGTVINPELQDEIVVTVIATGFDDKPTSHGRKSGSTGFGTS
                     VNTSSNATSKDESFTSNSSNAQATDSVSERTHTTKEDDIPSFIRNREERRSRRTRR"
BASE COUNT         5616 a         2074 c         2901 g         4610 t
ORIGIN      
        1 aaaattttga atagtatcaa aatctatagg aataatataa taataaaaat ctctacgcat
       61 gcaaatggtg agtgttaaaa atcttggtgt agtgttggtg tagttcaggt gcagtataga
      121 tgaaatttga gcacaaaatt acttaattaa aaatgtataa acaagtgtca gacgcgtgtc
      181 aattgtgtgt caaaaaattc tataacgaat aagcatgctt gttttaatta ctgtattttc
      241 ggggcttatg cgggggacgt tttggtgacg catcatacta tattactgaa attcaaaaca
      301 aaaagagccc cgtaatcacg gaactctttt gtttggtaat gcgtataaaa atacctataa
      361 acgtcctggg agggattcga acccccgacc gatggcttag aaggccattg ctctatccag
      421 ctgagctacc aggacacgtt taacaacaca agaattatta tatctaaatg aacttaaatt
      481 agcaatacct tgtaaataaa aaatgtttat atttttcact attatagagc tatttatcta
      541 aaaaggttca ataagactta aatacgaatt caggcaactt aattgtgtta aatacagttt
      601 tgaatgccta actgtatttc ttttctcttt aaaatacagt taagtacatt ataagatgtt
      661 gtgcggataa acaaactaat tgtatcaaat ttattttaaa ataacaacaa caaaacgata
      721 agcgaataac atttcggtga tttaaaagct acgcacgttt ttgttatctt caaatttaaa
      781 ttttaaggag tgttttcaat ggaaggttta tttaacgcaa ttaaagatac cgtaactgca
      841 gcaattaata atgatggcgc aaaattaggc acaagcattg tgagcatcgt tgaaaatggc
      901 gtaggtttat taggtaaatt attcggattc taatttcaat atgttatgta agtaatcagt
      961 attatttcaa aggtgaggga gagatttaaa tgactggact agcagaagca atcgcaaata
     1021 ctgtgcaagc tgcacaacaa catgatagtg tgaaattagg cacaagtatc gtagacatcg
     1081 ttgctaacgg tgtgggttta ctaggtaaat tatttggatt ctaatataat aactaatatt
     1141 ctttaaaata aactgggtga gcatacttta atgttatgca ctcagtttat tttatttgca
     1201 gaaatttgag cctctgttaa gatttagata catagacaat ataggagatg gggaaattgg
     1261 gatataaaaa tattttgata gactttgatg atacaattgt tgatttttat gatgcagaag
     1321 aatgggcgtt tcactatatg gcgaatgttt ttaatcataa agcaacaaag gatgattttt
     1381 taacatttaa aaaaatcaat caccaacatt gggaagcttt tcaacaaaat aaattaacga
     1441 agtctgaagt attatcagaa cgatttgtga attacttcaa acatcatcaa atggaagttg
     1501 atgggcatcg tgcagatgtg ttatttagaa atggattagc agaagctaaa gttaaatact
     1561 ttgatcaaac attagaaaca attgtcgaat tatcgaaaag acatgattta tatattgtta
     1621 ctaatggtgt aaccgaaacg caaaagagaa ggttaaatca gacgccgttg cataaatata
     1681 ttaaaaagat atttatatct gaagaaacag gatatcaaaa acctaatccg gaatttttta
     1741 attatgtttt taatgatatt ggtgaggatg aaagacagca ctcgattata gttggagatt
     1801 ctttaacatc tgacattcta ggtggaatca atgcgggtat agctacttgc tggtttaatt
     1861 ttagaggatt tgatcataat ccaggaatta tacctgatta tgaaattaat tcatggaaac
     1921 aactaaatga tattgtacgt taagacaaat aatttgaaaa tgatttactt atattagctt
     1981 aagaaagtaa ttgtaaaaaa gacactacga aaagatgcat tttcatgctc gtagtgtcga
     2041 atattattat cttatggttt ccacaataag acatcatgtc cctctatatt tttagcttcc
     2101 acatctgtaa atccatgatg taaaaagaaa tccttagaat catttctgcc aatggcttta
     2161 attggcatat tgaaactctt tgcaaaatca atcaattctg aagcgtaccc tctgttttga
     2221 tattttggta atacttctaa cttccataat aatatataat cttcaaaatc tgggaagtag
     2281 atttcttcga catcaccttt ttttagtaat gccattctag ctcctaattg atctccgaca
     2341 aatatgccat aaaatggtga atctgaactt gcatcaatca tttgaccgtt taactcattg
     2401 accatgtata agtctttgtt gccaaacgct ctaaagtttt cgaataattc gtcagttttg
     2461 taattaattt caagacgttt gatttcactc atatttatct accccttatt tgtttactac
     2521 tattattata cattaaacca cttgtgtttt tcattgattt gaaaatgtaa aacaatgaat
     2581 attataaatt ttccatgatt aaattgattt tcaagccgta aaattgtcaa tcaccggtat
     2641 agactttata ctatagtttg taatcgcgta cttaaggaga aaaaacatgg actgtaaagt
     2701 agttagttta aatgaaaaag atcagtttat accaaaaata aagagcagtg accctgtaat
     2761 aacaggatta tttcaatatg atgcagctca acaaactagt tttgaaaaaa ggatgtctaa
     2821 agaaaataat ggaagagaag cggcattagc gaatgttatt cgtgaatata tgagtgattt
     2881 aaagctttca agtgaacaag aattaaacat acaacattta gctaatggtt caaaagttgt
     2941 gattggtgga caacaagcag ggcttttcgg gggaccattg tatacattcc ataaaatatt
     3001 ttcaatcatt actttatcta aggaattaac ggatacacat aagcaacaag tagtaccagt
     3061 tttttggatt gcaggagaag atcatgattt cgatgaagtg aatcatacat ttgtttataa
     3121 cgaaaatcat gggtcgctgc ataaggttaa atatcataca atggagatgc cagagacgac
     3181 tgtctctaga tattatcctg ataaggctga gttgaaacaa actttaaaaa cgatgttcat
     3241 tcatatgaaa gaaactgttc atacacaagg tctactggag atttgtgaca gaattattga
     3301 ccaatatgac tcgtggactg atatgtttaa agcactactg catgaaacat ttaaagcata
     3361 tggcgttcta tttatagatg cgcagtttga gccgttaaga aaaatggaag cgcctatgtt
     3421 taaaaagatt ttgaaaaaac atcagttgct tgatgatgct tttagagcaa cacaacaacg
     3481 tactcaaaat caaggcttga atgcgatgat acaaacagat acaaatgttc atttattctt
     3541 acatgatgaa aatatgcgtc aattagtttc gtatgatggt aagcatttta aattaaataa
     3601 aacagataag acatatataa aggaagaaat tataaatatt gcggaaaatc aacctgaatt
     3661 attttctaat aatgtagtga caagaccatt aatggaagaa tggttattta acacggtggc
     3721 atttgttgga ggaccgagtg aaattaagta ctgggctgaa ctaaaagatg tatttgaact
     3781 atttgatgtt gaaatgccta tcgtgatgcc aaggcttaga attacttatt taaatgaccg
     3841 tatagaaaaa ttactttcga aatacaatat tccattagaa aaagtgttag tcgatggtgt
     3901 tgaaggagaa agaagtaagt ttattagaga acaagcatca catcaattta ttgaaaaggt
     3961 agaaggtatg attgaacaac agcgtcgtct aaacaaagac ttattagatg aagtggcggg
     4021 gaatcaaaat aatattaacc ttgtgaataa aaataatgaa attcatatac aacagtatga
     4081 ttatttgtta aaacgttatc ttttaaacat tgaaagagaa aacgacatca gtatgaagca
     4141 atttagagaa attcaagaaa cactccatcc aatgggagga ttacaagaaa gaatatggaa
     4201 tccacttcaa attttgaatg attttgggac agatgtgttc aagccctcca cctatccacc
     4261 actttcttac acttttgatc atattattat aaaaccttaa tataccaagg gtttagcccg
     4321 atttatctta atgataaatc gggcattttt ttgtttttta aaataaattt cacaaatttt
     4381 gtataaatag tggtggatag tggggagatg tggtaaatta tatataaggt gaggtgataa
     4441 aaaatgttca tgggagaata cgatcatcaa ttagatacaa aaggacgtat gattataccg
     4501 tccaagtttc gttatgactt aaatgagcgt tttattatca caagaggcct tgataaatgt
     4561 ttattcggtt acactctaga cgaatggcaa cagattgaag agaaaatgaa aaccttacct
     4621 atgacaaaaa aagacgcacg taagtttatg cgtatgttct tctctggtgc tgttgaagta
     4681 gaacttgata agcaagggcg tattaacatc cctcaaaact tgaggaaata cgctaattta
     4741 actaaagaat gtacagtaat cggtgtttca aatcgtattg agatttggga tagagaaact
     4801 tggaatgatt tctatgaaga atctgaagaa agtttcgaag atattgctga agatttaata
     4861 gattttgatt tttaaaatgg aggaattgaa gtgtttcatc atatcagcgt tatgttaaac
     4921 gaaaccattg attatttaaa tgtaaaagaa aatggtgtgt acattgactg tacgctaggt
     4981 ggagcgggac atgcccttta tttactaaat caattaaatg acgacggaag attaatagca
     5041 atcgatcaag accaaactgc aattgataat gctaaagagg tattaaagga tcatttgcat
     5101 aaggtgactt ttgttcatag caacttccgt gaattaactc aaatattaaa agacttaaac
     5161 attgaaaaag tagatggaat ttattacgac ttgggtgttt caagcccaca actcgacatt
     5221 ccagaacgag gattcagtta tcaccatgac gcaacattag acatgcgtat ggaccaaaca
     5281 caagaactaa cagcatatga aattgttaac aattggtcat atgaagcgtt agtgaagatt
     5341 ttttatcgct atggcgagga gaaattttca aaacagatag ctcgaagaat cgaagcacat
     5401 cgcgaacaac aaccaataac aacaacatta gaattagttg acattataaa agaaggtatt
     5461 cctgcaaaag caagaagaaa aggcggacat cctgcaaaac gagtatttca agcactacga
     5521 attgcagtaa acgatgaatt gtcagctttt gaagattcaa tagaacaagc gattgaatta
     5581 gtgaaagtag atggcaggat ttcggtaatc actttccatt ctttagaaga tcgtttatgt
     5641 aaacaggtgt tccaagaata tgaaaaaggt ccagaggtac caagaggatt accagttata
     5701 ccagaagcat atacacctaa gttaaagcgt gttaatcgta aaccgattac cgctacagaa
     5761 gaagatttag atgacaataa cagagcacga agcgcgaaat tacgtgtagc tgaaatactt
     5821 aaataaggag caatttataa tggctgtaga aaaagtgtac caaccatatg acgaacaagt
     5881 ttataatagt ataccgaagc aacaaccaca aactaagccc gaaaagaaga ctgtttcgag
     5941 aaaagtggtt gtacaattaa ctaaatttga aaaagtttta tacataactt tgattactgt
     6001 aattgctatg ttaagtattt atatgctatc tttaaaaatg gatgcgtatg atacgcgagg
     6061 aaagattgca gatttagatt ataaaataga taaacaatca agtgaaaaca gtgctttaca
     6121 atctgaaatc aaaaagaatt cttcttatga acgcatatac gaaaaggcta agaaacaggg
     6181 gatgagcctt gagaacgata atgtaaaggt agtgcgtagt aatggcgaag caaaaaatta
     6241 aaattaaaaa aaataaaata ggggcagtcc tacttgttgg tttattcgga ctgctctttt
     6301 ttatattggt tttaagaatt tcatatatca tgattactgg acattctaat ggtcaagatt
     6361 tagtcatgaa ggcaaatgaa aagtatttag ttaagaatgc acaacaacca gaacgaggaa
     6421 agatatatga tcgtaatggt aaagtgctag cagaagatgt agaaagatat aaacttgttg
     6481 cagtaataga taaaaaggcg agtgccaatt ctaaaaaacc taggcatgta gttgataaaa
     6541 aagagactgc aaagaaatta tctacagtca ttaatatgaa gccagaggaa attgaaaaga
     6601 gacttagtca aaagaaagct ttccaaattg aatttggacg caaaggaaca aatttaacgt
     6661 atcaggacaa attgaaaata gagaaaatga atttgcctgg tatttcttta ttgcctgaaa
     6721 cagaacgctt ttatccaaat ggcaattttg catcacactt aattggtaga gctcagaaaa
     6781 atccggatac tggtgaactt aaaggtgcac ttggagttga aaagattttt gatagttatt
     6841 taagtggatc taaaggatca ttgagatata ttcatgatat ttggggatat atcgcaccaa
     6901 atactaaaaa agagaagcag cctaaacgtg gtgatgatgt ccatttaaca atcgattcaa
     6961 atattcaagt atttgttgaa gaagctttag atggcatggt tgaaagatac cagccgaaag
     7021 atttatttgc ggttgtcatg gatgccaaaa ctggagaaat tttagcatac agtcagcgac
     7081 caacatttaa tcctgaaact ggtaaagact ttggtaaaaa gtgggcaaat gacctttatc
     7141 aaaacacata cgagcctgga tcaacattta aatcatatgg gttagcagct gctattcaag
     7201 aaggtgcttt tgatcctgat aagaaatata aatctggaca tagagatatt atgggttcac
     7261 gtatttcaga ctggaataga gtcggttggg gtgaaatccc aatgtcactc ggatttactt
     7321 attcatctaa tacattgatg atgcatttac aagatttagt tggtgcagac aaaatgaaat
     7381 cttggtatga acgatttgga tttggaaaat caactaaagg tatgtttgat ggagaagcac
     7441 ctggtcaaat tggatggagt aatgagttgc aacaaaaaac gtcatcattt ggtcaatcga
     7501 caacagtaac acctgttcaa atgttacaag cgcaatcagc gttctttaat gatggtaata
     7561 tgttaaaacc atggtttgtg aatagcgttg aaaatcctgt tagtaaaaga caattttata
     7621 aagggcaaaa acaaatcgca ggcaaaccaa taacaaaaga tactgctgaa aaagttgaaa
     7681 agcaattgga tttagttgtg aatagtaaga agagtcacgc tgcaaactat cgtattgatg
     7741 gttatgaggt cgaaggtaag actggtacag cacaagtcgc tgcacctaat ggtggtggat
     7801 acgttaaagg tccaaaccca tattttgtaa gttttatggg tgacgcgccg aagaaaaatc
     7861 ctaaagttat tgtatacgct ggtatgagct tggcacaaaa aaatgaccaa gaagcttatg
     7921 aattaggtgt tagtaaagcg tttaaaccaa taatggaaaa tactttgaaa tatttaaatg
     7981 taggtaaatc aaaagatgac acatctaatg cagagtatag taaagtgcca gatgttgaag
     8041 gtcaagacaa acaaaaagct attgataatg tgagtgcaaa atcattagaa ccagttacta
     8101 ttggttctgg cacacaaata aaagcacaat ctataaaagc agggaataaa gtcttacctc
     8161 atagtaaagt actgttatta acagatggag acttaactat gcctgacatg tcaggatgga
     8221 cgaaagaaga tgtcattgct tttgaaaacc taacaaatat taaagtaaat ttaaaaggta
     8281 gcggttttgt gtcccaccaa tcaattagta agggacaaaa acttactgaa aaagataaaa
     8341 tagacgtaga attttcatca gagaatgtag acagcaattc gacgaataat tctgattcaa
     8401 attcagatga taagaagaaa tctgacagta aaactgacaa ggataagtcg gactaacaag
     8461 aatggctaca ttaaattagg atgtattgtc actatttgtt tttactaaag taatgctgtg
     8521 atagaaaaca acatcttcat ttaatcttta taaataagtg ttgatagaga attttaaggt
     8581 agacgaggtc agattaactt gtttgttata ggcaaacttt ttatactggc ctcttatgcc
     8641 gtaaaaaaga aaaattataa attttgatta caactttgtt tatttaattg aaaaattgat
     8701 aatatattag tggttaaaaa agtctggatt aagaagagga gatagttatg atttttgtat
     8761 atgcgttatt agcgctagtg attacatttg ttttggtacc tgttttaata cctacattaa
     8821 aaaggatgaa atttggtcaa agtattcgag aagaaggtcc acaaagccat atgaagaaga
     8881 ctggtacacc aacgatgggt ggactaacat ttctattaag tattgtgata acgtctttgg
     8941 tggctattat atttgtagat caagctaatc caatcatact gttattattt gtgacgattg
     9001 gttttgggtt aattggtttt atagatgatt atattattgt tgttaaaaag aataaccaag
     9061 gtttaacaag taaacagaag tttttggcgc aaattggtat tgcgattata ttctttgttt
     9121 taagtaatgt gtttcatttg gtgaattttt ctacgagcat acatattcca tttacgaatg
     9181 tagcaatccc actatcattt gcatatgtta ttttcattgt tttttggcaa gtaggttttt
     9241 ctaatgcagt aaatttaaca gatggtttag atggattagc aactggactg tcaattattg
     9301 gatttacaat gtatgccatc atgagctttg tgttaggaga aacggcaatt ggtattttct
     9361 gtatcattat gttgtttgca cttttaggat ttttaccata taacattaac cctgctaaag
     9421 tgtttatggg agatacaggt agcttagctt taggtggtat atttgctacc atttcaatca
     9481 tgcttaatca ggaattatca ttaattttta taggtttagt attcgtaatt gaaacattat
     9541 ctgttatgtt acaagtcgct agctttaaat tgactggaaa gcgtatattt aaaatgagtc
     9601 cgattcatca tcattttgaa ttgataggat ggagcgaatg gaaagtagtt acagtatttt
     9661 gggctgttgg tctgatttca ggtttaatcg gtttatggat tggagtgcat taagatgctt
     9721 aattatacag ggttagaaaa taaaaatgta ttagttgtcg gtttggcaaa aagtggttat
     9781 gaagcagcta aattattaag taaattaggt gcgaatgtaa ctgtcaatga tggaaaagac
     9841 ttatcacaag atgctcatgc aaaagattta gaatctatgg gcatttctgt tgtaagtgga
     9901 agtcatccat taacgttgct tgataataat ccaataattg ttaaaaatcc tggaatacct
     9961 tatacagtat ctattattga tgaagcagtg aaacgaggtt tgaaaatttt aacagaagtt
    10021 gagttaagtt atctaatctc tgaagcacca atcatagctg taacgggtac aaatggtaaa
    10081 acgacagtta cttctctaat tggagatatg tttaaaaaaa gtcgcttaac tggaagatta
    10141 tccggcaata ttggttatgt tgcatctaaa gtagcacaag aagtaaagcc tacagattat
    10201 ttagttacag agttgtcgtc attccagtta cttggaatcg aaaagtataa accacacatt
    10261 gctataatta ctaacattta ttcggcgcat ctagattacc atgaaaattt agaaaactat
    10321 caaaatgcta aaaagcaaat atataaaaat caaacggaag aggattattt gatttgtaat
    10381 tatcatcaaa gacaagtgat agagtcggaa gaattaaaag ctaagacatt gtatttctca
    10441 actcaacaag aagttgatgg tatttatatt aaagatggtt ttatcgttta taaaggtgtt
    10501 cgtattatta acactgaaga tctagtattg cctggtgaac ataatttaga aaatatatta
    10561 gcagctgtgc ttgcttgtat tttagctggt gtacctatta aagcaattat tgatagttta
    10621 actacatttt caggaataga gcatagattg caatatgttg gtactaatag aactaataaa
    10681 tattataatg attccaaagc aacaaacacg ctagcaacac agtttgcctt aaattcattt
    10741 aatcaaccaa tcatttggtt atgtggtggt ttggatcgag ggaatgaatt tgacgaactc
    10801 attccttata tggaaaatgt tcgcgcgatg gttgtattcg gacaaacgaa agctaagttt
    10861 gctaaactag gtaatagtca agggaaatcg gtcattgaag cgaacaatgt cgaagacgct
    10921 gttgataaag tacaagatat tatagaacca aatgatgttg tattattgtc acctgcttgt
    10981 gcgagttggg atcaatatag tacttttgaa gagcgtggag agaaatttat tgaaagattc
    11041 cgtgcccatt taccatctta ttaaagggtg tgagtattga tggatgataa aacgaagaac
    11101 gatcaacaag aatcaaatga agataaagat gaattagaat tatttacgag gaatacatct
    11161 aagaaaagac ggcaaagaaa aagatcaaag gctacacatt tttctaatca aaataaagat
    11221 gatacatctc aacaagctga ttttgatgaa gaaatttact tgataaataa agacttcaaa
    11281 aaagaagaaa gcaatgataa aaataatgat tctgcttcta gtcatgcgaa tgataataat
    11341 atcgatgatt ctacagactc taatattgaa aatgaggatt atagatataa tcaagaaatt
    11401 gacgaccaaa atgaatcgaa tgtaatttca gtcgacaacg aacaacctca atcagctcct
    11461 aaagaacaaa atagcgactc gattgatgag gaaacagtaa cgaaaaaaga acgaaaaagt
    11521 aaagtaacac aattaaagcc attaacactt gaagaaaagc ggaagttaag acgtaagcga
    11581 caaaagcgaa tccaatacag tgttattaca atattggtat tgttgattgc tgttatatta
    11641 atttacatgt tttcaccact tagtaaaatt gcgcatgtaa atataaatgg aaataatcac
    11701 gttagtactt caaagataaa caaagtttta ggtgttaaaa atgattcaag gatgtatacg
    11761 tttagtaaaa aaaatgctat taatgatctc gaagagaatc cattaatcaa aagtgttgag
    11821 atacacaagc aattaccaaa cacattaaac gtagatatca cagaaaatga aattattgct
    11881 ttagtgaaat ataaaggtaa atatttacct ttattagaaa atggtaaatt gcttaaaggt
    11941 tcaaatgatg tcaaaattaa tgatgcacct gtcatggatg gtttcaaagg tacaaaagaa
    12001 gatgatatga ttaaggcgtt atctgaaatg acacctgaag ttagacgata tattgccgaa
    12061 gtgacatacg ccccaagtaa aaacaaacaa agcagaattg aattgtttac gacagatgga
    12121 cttcaagtaa tcggtgatat ttcgacgata tctaagaaaa tgaaatatta tccgcagatg
    12181 tcacaatcat tatcaaggga tagttcgggt aaactaaaaa cacgaggcta tattgattta
    12241 tcagtcggtg cttcatttat cccataccgt ggaaacacgt ctagtcaatc agaaagcgat
    12301 aaaaatgtga ctaaatcatc tcaagaggaa aatcaagcaa aagaagaatt acaaagcgtt
    12361 ttaaacaaaa ttaacaaaca atcaagtaag aataattaaa aaattttgat attgtctatg
    12421 tttatagttc acaagccatt caacgtattg taaactaagg atagtgtatt tttttaatag
    12481 taatttgtca ggaggtgcct atctatggaa gaacattact acgtaagtat tgatattgga
    12541 tcatcaagcg taaaaacaat agtaggcgag aaatttcaca atggtataaa tgtgataggt
    12601 acaggacaaa cctacacgag cggtataaaa aatggtttaa ttgatgattt tgatattgcg
    12661 cgacaagcaa tcaaagacac aattaaaaag gcatcaatcg cttcgggtgt tgatattaaa
    12721 gaagttttcc tgaaattacc tatcattgga acggaagttt atgatgaatc aaatgaaatc
    12781 gacttttatg aggatacaga aatcaacggt tcacatatcg aaaaagtatt agaaggtatt
    12841 agagaaaaaa atgatgtgca agaaacagaa gtaattaatg tgttcccgat tcgttttata
    12901 gtcgataaag aaaatgaggt ttcagaccct aaagaattaa ttgccagaca ttcattaaag
    12961 gttgaagcag gcgtaattgc tattcaaaaa tcgattttaa ttaatatgat taaatgcgta
    13021 gaagcatgtg gtgttgatgt attagatgtt tactctgatg catataacta tggttcaatc
    13081 ctaacagcta ctgaaaaaga gttaggtgca tgtgtcattg atattggtga agacgttacg
    13141 caagttgctt tttatgaacg cggtgaatta gtagatgctg attctatcga aatggcaggg
    13201 cgtgatatta cagacgatat tgcacaagga ttaaacactt cttatgaaac tgctgaaaaa
    13261 gttaaacacc aatatggtca tgcattctat gattctgctt cagatcaaga tatcttcact
    13321 gttgaacagg ttgatagtga tgaaacagta cagtatactc aaaaagattt gagtgacttt
    13381 attgaagcgc gtgtagaaga aatattcttc gaagtatttg atgttttaca agatttagga
    13441 ttaacaaaag taaatggtgg gtttattgta actggtggat ctgcaaactt acttggcgta
    13501 aaagaattat tatcagatat ggtaagtgaa aaagttagaa ttcacacgcc atcacaaatg
    13561 ggaattagaa aacctgaatt ttcttcagca atttctacaa tttctagtag tatcgctttt
    13621 gatgagttat tagattatgt tacaattaat tatcatgata atgaagaaac tgaagaagat
    13681 gttattgatg tgaaagacaa agataacgaa tctaaattag gcggatttga ttggtttaaa
    13741 cgtaaaacaa acaaaaaaga tactcatgaa aatgaagtag agtcaacaga tgaagaaatt
    13801 tatcaatcag aagataatca tcaggaacat aaacagaatc atgaacatgt tcaagacaaa
    13861 gataaagata aagaagaaag taaattcaaa aaactaatga aatctctatt tgaatgatta
    13921 ttggccaata aaactaggag gaaatttaaa tgttagaatt tgaacaagga tttaatcatt
    13981 tagcgacttt aaaggtcatt ggtgtaggtg gtggcggtaa caacgccgta aaccgaatga
    14041 ttgaccacgg aatgaataat gttgaattta tcgctatcaa cacagacggt caagctttaa
    14101 acttatctaa agctgaatct aaaatccaaa tcggtgaaaa attaacacgt ggtttaggag
    14161 caggagctaa tcctgaaatc ggtaaaaaag ctgcagagga atctcgtgaa caaattgaag
    14221 atgcaatcca aggtgcagac atggtatttg ttacttctgg tatgggtggc ggaactggta
    14281 ctggtgcagc accagtcgtt gctaaaattg caaaagaaat gggcgcatta actgttggtg
    14341 ttgtaactcg tccatttagt tttgaaggac gtaaacgtca aactcaagct gctgctggag
    14401 tagaagctat gaaagctgca gtagatacat taatcgttat accaaatgac cgtttattag
    14461 atatcgttga caaatctacg ccaatgatgg aagcatttaa agaagctgac aacgtgttac
    14521 gccaaggtgt acaaggtatc tcagacttaa tcgctgtttc tggtgaagta aacttagact
    14581 ttgcagacgt taagacaatt atgtctaacc aaggttctgc attaatgggt attggtgttt
    14641 cttctggtga aaatagagcg gtagaagctg ctaaaaaagc aatctcttct ccattacttg
    14701 aaacatctat cgttggtgca caaggtgtgc ttatgaatat tactggtggc gagtcattgt
    14761 cattatttga agcacaagag gctgctgata ttgtccaaga tgctgcagat gaagacgtta
    14821 atatgatttt cggtacagtt attaatcctg aattacaaga tgagattgtt gtaacagtta
    14881 ttgcaactgg ttttgatgac aaaccaacat cacatggtcg taaatctggt agcactggat
    14941 tcggaacaag cgtaaatact tctagcaatg caacttctaa agatgaatca ttcacttcaa
    15001 attcatcaaa tgcacaagca actgatagtg taagtgaaag aacacataca actaaagaag
    15061 atgatattcc tagcttcatt agaaatagag aagaaagacg ttcaagaaga acaagacgtt
    15121 aatcggttaa tatatataca caaataattc aacacaaatc atcagataac atatctgatg
    15181 atttttttac taatttttag a
//
LOCUS       DAKTIO010000052        15155 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.052, whole genome shotgun sequence.
ACCESSION   DAKTIO010000052 DAKTIO010000000
VERSION     DAKTIO010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15155
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.052"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            15..293
                     /locus_tag="O0293_001219"
     CDS             15..293
                     /locus_tag="O0293_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04197 family type VII secretion effector"
                     /protein_id="HCY0280108.1"
                     /translation="MMSTVQSDIFKTNSASSSIKSAVETCNNVSKPDKDESTTVSGNN
                     NAHSVIDDLMSKNQSVAEAIRTASDNIQKVGEAFDQTDVMIGNEIGKN"
     gene            308..676
                     /locus_tag="O0293_001220"
     CDS             308..676
                     /locus_tag="O0293_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280109.1"
                     /translation="MSNKLDEINKIITAKHEQMDDLYDEKREVKALIDESDALNHSID
                     QLYQHLGERYYSSNMASRMEQFRDEFHFAKRRSTEALYEQQQQIQHGIRKVEEEMIDL
                     EMRRNVEIETVTKEENKWKQ"
     gene            664..960
                     /locus_tag="O0293_001221"
     CDS             664..960
                     /locus_tag="O0293_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HCY0280110.1"
                     /translation="METIGSIIYLKEGSQKLMIINRGPIVEIENQKYMFDYSACKYPI
                     GVVEDEIYYFNEENIDSVIFKGYSDQDEVRFQELFENMKQNLDSEIQRGEVTQQ"
     gene            1294..2400
                     /locus_tag="O0293_001222"
     CDS             1294..2400
                     /locus_tag="O0293_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HCY0280111.1"
                     /translation="MQHHKVAIIGAGAAGIGMAITLKDFGITDVIILEKGTVGHSFKH
                     WPKSTRTITPSFTSNGFGMPDMNAISMDTSPAFTFNEEHISGETYAEYLQVVANHYEL
                     NIFENTVVTNISVDDAYYTIATTTEIYHADYIFVATGDYNFPKKPFKYGIHYSEIEDF
                     DNFNKGQYVVIGGNESGFDAAYQLAKNGSDIALYTSTTGLNDPDADPSVRLSPYTRQR
                     LGNVIKQGARIEMNVHYTVKDIDFNNGQYHISFDSGQSVLTPHEPILATGFDATKNPI
                     VQQLFVTTNQDIKLTTHDESTRYPNIFMIGATVENDNAKLCYIYKFRARFAVLAHLLT
                     QREGLPAKQDVIENYQKNQMYLDDYSCCEVSCTC"
     gene            2394..3734
                     /locus_tag="O0293_001223"
     CDS             2394..3734
                     /locus_tag="O0293_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HCY0280112.1"
                     /translation="MLEVKYDMRTGHYTPIPNEPHYLVISHADKLTATEKAKLRLLII
                     KQKLDISLAESVVSSPIASEHVIEQLTLFQHERRHLRPKISATFLAWLLIFLMFALPI
                     GIAYQFSDWFQNQYVSAWIEYLTQTTLLNHDILQHILFGDYGVLSLGTYSLVWALPVV
                     ILISLSTAIIDQTGLKSWMIWAIEPSMLWIGLQGNDIVPLLEGFGCNAAAISQAAHQC
                     HTCTKTQCMSLISFGSSCSYQIGATLSIFSVAGKSWLFMPYLILVLLGGILHNRIWLK
                     KNDQQLSVPLPYDRQLHMPNIRQMLLQMWQNIQMFIVQALPIFITICLIVSILSLTPI
                     LNVLSQIFTPILSLLGISSELSPGILFSMIRKDGMLLFNLHQGALLQGMTATQLLLLV
                     FFSSTFTACSVTMTMLLKHLGGQSALKLIGKQMVTSLSLVIGVGIIVKIVMLII"
     gene            3832..4722
                     /locus_tag="O0293_001224"
     CDS             3832..4722
                     /locus_tag="O0293_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCY0280113.1"
                     /translation="MKIVIIGGFLGGGKTTVLNHLLAESLKESLKPAVIMNEFGKMSV
                     DGALVSEDIPLSELTEGCICCAMKADVSEQLHQLYLKEQPDIVFIECSGIAEPVSVLD
                     ACLTPILAPFTTITHMIGVIDASMYKHIKSFPKDIQGLFYEQLAYCSVLFVNKIDSAD
                     VETTSKLLKDLEVINPEADIQVGMHGSVTLPISVRQMTATSDNKHKSLHQMINHQFVQ
                     SPVKCTKAEFIKRLACLPSHIYRLKGFMTFEDTAHTYLIQFTQGQYELTPVAFSKKVP
                     EYLVLIGKGISKEDYQCLEQ"
     gene            complement(4869..5699)
                     /locus_tag="O0293_001225"
     CDS             complement(4869..5699)
                     /locus_tag="O0293_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0280114.1"
                     /translation="METLELQGAKLRYHQVGQGPVLIFIPGANGTGDIFLPLAEQLKD
                     HFTVVAVDRRDYGESELTEPLPDSASNPDSDYRVKRDAQDIAELAKSLSDEPVYILGS
                     SSGSIVAMHVLKDYPEVVKKIAFHEPPINTFLPDSTYWKDKNDDIVHQILTEGLEKGM
                     KTFGETLNIAPIDAKMMSQPADTEEGRIEQYKRTMFWLEFEIRQYTHSNITLDDFTKY
                     SDKITLLNGTDSRGSFSQDVNFYINKETGIPIVDIPGGHLGYIQKPEGFADVLLNMWG
                     "
     gene            complement(5719..6729)
                     /locus_tag="O0293_001226"
     CDS             complement(5719..6729)
                     /locus_tag="O0293_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCY0280115.1"
                     /translation="MKSITFEEHYVIEDIQKETMNAISADPKGVPMKVMLEGLEKKTG
                     FTNADELSHHDERIQFMNNQDVQIQVLSYGNGSPSNLVGQKAIELCQKANDQLANYIA
                     QYPNRFVGFATLPINEPEAAAREFERCINDLGFKGALIMGRAQDGFLDQDKYDIIFKT
                     AENLDVPIYLHPAPVNSDIYQSYYKGNYPEVTAATFACFGYGWHIDVGIHAIHLVLSG
                     IFDRYPKLNMIIGHWGEFIPFFLERMDEALFAEHLNHPVSYYFKNNFYITPSGMLTKP
                     QFDLVKKEVGIDRILYAADYPYIEPEKLGVFLDELGLTDEEKEKISYTNGAKLLGLSP
                     NN"
     gene            complement(6827..7531)
                     /locus_tag="O0293_001227"
     CDS             complement(6827..7531)
                     /locus_tag="O0293_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCY0280116.1"
                     /translation="MTKIVLITGGNKGLGYASAEALKALGYKVYIGSRNDVRGQQASQ
                     KLGVHYVQLDVTSDYSVKNAYNMIAEKEGRLDILINNAGISGQFSAPSKLTPRDVEEV
                     YQTNVFGIVRMMNTFVPLLEKSEQPVVVNVSSGLGSFGMVTNPETAESKVNSLAYCSS
                     KSAVTMLTLQYAKGLPNMQINAADPGATNTDLVGDFSNNSKHVSEGIKPIIQLATIGA
                     DGPTGTFINGDGEMPW"
     gene            complement(7524..8081)
                     /locus_tag="O0293_001228"
     CDS             complement(7524..8081)
                     /locus_tag="O0293_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCY0280117.1"
                     /translation="MRKDAKENRQRIEEIAHKLFDEEGVENISMNRIAKELGIGMGTL
                     YRHFKDKSDLCYYVIQRDLDIFITHFKQIKDDYHSNYEVMQVSLDYLLQFKIDNKALL
                     QCIEAGNNKLRFYQSAFYQELFDFYYDLFKSDDDTYTKFKTDMLLQSLSTSVFAFQIE
                     HRHISIEAYRNYLLNIYLDEVGRND"
     gene            8212..8511
                     /locus_tag="O0293_001229"
     CDS             8212..8511
                     /locus_tag="O0293_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HCY0280118.1"
                     /translation="MSLNKEQRRITAEELQAHFEASTLSVQMIAEKLNVTTEDVEKVL
                     AMTAPLGIFSHQLQRFIHLVWDVRDVINDNIKGNGQTPEPYTYLKGEKEDYWFLR"
     gene            8531..9352
                     /locus_tag="O0293_001230"
     CDS             8531..9352
                     /locus_tag="O0293_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HCY0280119.1"
                     /translation="MKDILVIGATGKQGNAVVKQLLEDGWYVSALTRNKNNRKLSDIG
                     HPHLSIVEGDLSDNVSLQSAMKGKYGLYSIQPIVKDDVSEELRQGMKIIEIAEQENIQ
                     HIVYSTAGGVNRNRTGPHFEVLAKIENRLMESNINATVIKPSFFMDNFLRIAKVEDER
                     ITLPEFINPNIKFTMISSIDIAKIASYIFAHPQSFTHQSIEIGSDEVTLSEAATIFSE
                     VTGKSTVIEGEFVSGVAEKQWLEEKGYEVDFELMAEINPTRLSLSDWLKVQNYNK"
     gene            9369..9746
                     /locus_tag="O0293_001231"
     CDS             9369..9746
                     /locus_tag="O0293_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0280120.1"
                     /translation="MIQSMWFNLHVQDLEKSAQFYKALGFKINRNPQMLDKMVGIQIG
                     QTTVILIENKHFQNVSQQSLNTEPNEVMISLGVNTNEEVDQLVNKVKEAGGTVVQEPT
                     VSQGFYGAMFKDLDGHHFNFLVC"
     gene            complement(9896..10126)
                     /locus_tag="O0293_001232"
     CDS             complement(9896..10126)
                     /locus_tag="O0293_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HCY0280121.1"
                     /translation="MKILDRINELANKEKVQPLTVAEKQEQHALRQDYLSMIRGQVLT
                     TFSTIKVVDPIGQDVTPDKVYDLRQQYGYIQN"
     gene            complement(10346..10846)
                     /locus_tag="O0293_001233"
     CDS             complement(10346..10846)
                     /locus_tag="O0293_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280122.1"
                     /translation="MTSIFVFAYLNNTSGNMLFRQQMICACLLANVRHSSIVCLNNIC
                     CIVDMRCLNTKIGGIDVSETDEPQGFERTHNILNINQSSLGVVTYITNKLKSTLKQHI
                     IIARGKKRIDYRLSYNFYTTYYDNVEIKKIDCEYTYAMPIAILIRHTMSFDNAHFFAQ
                     LRHPVL"
     gene            complement(11044..11613)
                     /locus_tag="O0293_001234"
     CDS             complement(11044..11613)
                     /locus_tag="O0293_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCY0280123.1"
                     /translation="MMESKSIDPRIVRTKQLLVDAFLKISREKKLSQITVKDITDIAT
                     LNRATFYAHFTDKEDLLDYTLSVTILKDLNDNLSISNVINEKVLRNIFISIASYIKDA
                     AKSCELNSEAFCNKAHQRINNELEDIFAIMLENSYPEHQRDIIVNSASFLAAGISGLA
                     LHWFNTSQETADVFIDRNLPFLIHHIAHF"
     gene            11768..11896
                     /locus_tag="O0293_001235"
     CDS             11768..11896
                     /locus_tag="O0293_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280124.1"
                     /translation="MDFKNEDKIKVYCNNMFEKLPISLSENSEFCDILFFIVLSYR"
     gene            12023..13078
                     /locus_tag="O0293_001236"
     CDS             12023..13078
                     /locus_tag="O0293_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HCY0280125.1"
                     /translation="MTSIKTITPKDFIFRVLSGVAIGIVAGLVPNAILGEIFKYFMDY
                     HPIFKTLLGVVVAIQFTVPALIGALVAMKFDLSPLAIAVVASAAYVGSGAAQFKNGAW
                     MITGIGDLINTMITAAIAVLFILLIQHRVGSMALIVFPTVVGGISGAIGVLILPYTKM
                     ITTAIGNMVNGFTELQPIVMSILISMVFSLIIISPLSTVAIAFAIGITGLAAGSASIG
                     ISATEAVLIIGTSKVNRLGVPLSVFFGGVKMMIPNMVKYPILMLPILTTAIVSGLVSA
                     LVGIHGTKESAGFGFIGMVGPINAFKFMEVDSAWLSVLLIVVAFFVVPFVTAWLADII
                     YRKVFRLYTNDIFKFMG"
     gene            13686..14387
                     /gene="isaA"
                     /locus_tag="O0293_001237"
     CDS             13686..14387
                     /gene="isaA"
                     /locus_tag="O0293_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase IsaA"
                     /protein_id="HCY0280126.1"
                     /translation="MKKTIMASSLAVALGVTGYAAGTGHQAHAAEVNVDQAHLVDLAH
                     NHQDQLNAAPIKDGAYDIHFVKDGFQYNFTSNGTTWSWSYEAANGQTAGFSNVAGADY
                     TTSYNQGSNVQSVSYNAQSSNSNVEAVSAPTYHNYSTSTTSSSVRLSNGNTAGATGSS
                     AAQIMAQRTGVSASTWAAIIARESNGQVNAYNPSGASGLFQTMPGWGPTNTVDQQINA
                     AVKAYKAQGLGAWGF"
     gene            14663..15151
                     /locus_tag="O0293_001238"
     CDS             14663..15151
                     /locus_tag="O0293_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0280127.1"
                     /translation="MAINIIEYNRSYKEELIEFILSIQKNEFNIKIDRDDQPDLENIE
                     HNYLNNGGQFWLAINNHQNIVGTIGLIRLDNNMSALKKMFVDKGYRNLKIGKKLLDKV
                     IMTCKEQNIDGIYLGTIDKFISAQYFYSNNGFREIKRGDLPSSFPKLDVDNRFYYRNL
                     KD"
BASE COUNT         5089 a         2352 c         2765 g         4949 t
ORIGIN      
        1 ggagagaggt gtaaatgatg agtacagttc aaagtgatat ttttaagacc aatagtgcat
       61 catcatctat taaaagcgct gttgaaacat gtaataatgt gtcgaaaccg gataaagatg
      121 aaagtacaac agtaagtgga aataataatg ctcatagtgt gatagatgat ttgatgagta
      181 agaatcaatc tgttgctgaa gcaatacgaa ctgcgagcga taatatacaa aaagttggtg
      241 aggcttttga ccaaactgac gtaatgattg gtaatgaaat tggtaaaaat taaaacgtgg
      301 tgaaatgatg tcgaataaac tggatgaaat caataaaata atcacagcga aacatgagca
      361 aatggatgac ttatatgatg aaaagcgaga ggttaaagca ttgatagatg aaagtgatgc
      421 gcttaatcat tcgatagatc aattatatca acatttaggt gagcgttatt atagtagcaa
      481 tatggctagt cgtatggaac agttccgcga tgaatttcat tttgcgaaac gacgttcaac
      541 ggaagcgtta tacgagcagc aacagcaaat tcaacatggc attcgtaaag tggaagaaga
      601 gatgattgac ttggaaatgc gaaggaatgt tgaaattgag acggtgacaa aggaggaaaa
      661 taaatggaaa caataggaag cattatttat ttaaaagaag gttcgcaaaa gttaatgatt
      721 attaatagag gaccaattgt agaaattgaa aatcaaaagt atatgtttga ctattctgca
      781 tgtaaatatc cgattggtgt tgtagaagat gaaatttatt attttaacga ggaaaatata
      841 gattcagtta tttttaaagg ttattctgat caagatgagg ttagatttca agagttgttt
      901 gaaaatatga aacaaaattt ggatagtgaa atacaacgtg gagaagttac acaacaataa
      961 agaaatactt tttctttatt ggggtgggac gacgaaataa attttgtaaa aatatgattt
     1021 ctgtctcacc gccaaaatgt tatctccaag tactgaggct ggatgatacc atgaaatgtc
     1081 attcataatc atttgaacta aacgtagcag ccttaaattt taaaaaaaga cacataccaa
     1141 cttccgaaat gtagatgaat tctctacaat aacggaagtt tttcttttaa tattgaaatt
     1201 tctcaaggat aggtctatac tttataaatc gtaattatta cgatttataa tcaaaaacaa
     1261 taacttgaaa tagatcattg agggagtgtt aatatgcaac atcataaagt ggctattatc
     1321 ggtgccggtg ctgcaggtat aggtatggcc attaccttaa aagatttcgg tataacagat
     1381 gtcattattt tagaaaaagg aacagtagga cattcattta aacattggcc gaaatcgacc
     1441 cgtacgatca cgccatcatt tacgtctaat ggatttggca tgcctgatat gaatgcaatt
     1501 tccatggata cttcaccagc atttacattt aatgaagaac atatttccgg agaaacatat
     1561 gctgaatatt tacaagtggt tgccaaccat tacgagctga atatctttga aaatacagtt
     1621 gtcacaaata tatctgtaga tgatgcatat tatacgattg caacgacaac agagatatat
     1681 cacgcggatt atatctttgt cgcaacaggt gattataatt tccctaaaaa gccatttaaa
     1741 tatggtattc attatagtga aattgaagac tttgataact ttaataaggg gcaatatgtg
     1801 gttatcggag gtaatgaaag tggctttgat gctgcatatc aacttgcaaa aaatggctct
     1861 gacatcgcac tttatactag cacaaccggt ttaaatgatc cggatgctga tcctagtgtt
     1921 agattgtcac cttatacacg tcagcgacta ggtaatgtca ttaagcaagg tgctcgcatc
     1981 gaaatgaatg tacattatac agttaaagat attgatttta acaatggaca gtatcatatc
     2041 agttttgata gcggacaaag tgtgcttaca cctcatgaac caatactagc aactggcttt
     2101 gatgcaacaa aaaatccaat cgttcaacaa ttatttgtga caacaaatca agatattaaa
     2161 ttaacaacac atgatgaatc gacacgttat ccgaatattt ttatgattgg tgcaacagtt
     2221 gaaaatgata atgccaaatt atgctatatc tataaattta gagcgcgatt tgcagtactt
     2281 gcacatcttt taacacagcg ggaaggctta ccagctaaac aagatgtcat tgaaaattat
     2341 caaaaaaatc aaatgtattt agatgattat tcatgttgtg aagtgtcatg cacatgttag
     2401 aagtgaaata tgatatgaga actgggcatt atacgcccat acctaatgaa cctcattatt
     2461 tggttattag tcatgcggat aaacttaccg caacagaaaa agcgaaatta agattattaa
     2521 tcataaaaca gaaattagat atttcattgg cagaaagtgt agtttcttcg cctatagcga
     2581 gtgaacatgt gatagaacaa ttgacactat ttcaacatga gcgacgacat ttaagaccta
     2641 aaataagtgc gacattttta gcctggttgt tgatattttt aatgtttgca ttgccaatcg
     2701 gtatcgctta tcaattttca gattggtttc aaaatcagta tgtgtcagca tggatagaat
     2761 atttaactca aacaacattg ctcaatcacg atatattaca gcatatatta tttggtgatt
     2821 atggtgtgct atcacttgga acatattcgc tcgtatgggc attgccggtt gtaatattga
     2881 ttagtttatc aactgctata attgatcaaa caggactcaa atcatggatg atatgggcaa
     2941 ttgaaccgtc aatgttatgg ataggattac aaggtaatga tatcgtgcca ctattagaag
     3001 ggtttggatg taatgcagca gctatttcac aagcagcaca ccaatgccat acctgcacga
     3061 agacacagtg tatgagttta ataagctttg gtagttcttg tagttatcaa ataggtgcga
     3121 cattatctat ttttagtgta gctggaaagt catggctatt tatgccgtac ttaatattag
     3181 tacttttagg tggcatctta cataatagga tatggttgaa aaagaatgat caacaactta
     3241 gcgttccgct accttatgat aggcaattac atatgccaaa tatacgtcaa atgttgctac
     3301 aaatgtggca aaatatacaa atgtttatcg ttcaagcgct acctattttt atcacaatct
     3361 gtcttattgt tagtatttta tcactaacgc caattttgaa tgttttatca caaatattta
     3421 cacctatatt atcgttatta ggcatctcgt cagaattgtc accagggatt ttattttcaa
     3481 tgattcgaaa agacggcatg ctcttgttta atttgcatca gggcgcctta ttacaaggaa
     3541 tgacagcaac acagttacta ctacttgtgt tttttagttc aacatttaca gcgtgctcgg
     3601 tcacaatgac gatgcttttg aaacatttag gtggtcagtc agcactaaaa ttaattggaa
     3661 agcaaatggt gacatcattg tctttagtta ttggtgtagg catcattgtt aaaatagtaa
     3721 tgctgattat ttaaaaaaaa tgaactataa ctgaatatag agtcatgtca gtcaatagga
     3781 gatctatctt ggaatatgct attcatatga agtataagag gagagtcgca gatgaaaata
     3841 gttattatag gtgggttttt aggtggcggt aaaacgactg tcttaaatca tttgctcgct
     3901 gaatcattaa aggaatcgct gaaaccagca gtcatcatga atgaatttgg gaaaatgagt
     3961 gttgatggtg ccttagtatc tgaagacata cctttaagtg aactgacaga ggggtgtatc
     4021 tgttgtgcaa tgaaagcaga tgtatcagaa cagttacatc aattatattt aaaagagcaa
     4081 ccagacattg tatttattga atgtagtggg attgcagaac cggtctctgt cttagatgct
     4141 tgtttaacgc ctattttagc tccgtttaca acaattacac atatgattgg tgtaatagac
     4201 gcaagcatgt ataaacacat taaatcattc cctaaagaca tccaaggctt attttatgag
     4261 caattagcat attgttctgt cttatttgtt aataaaatag attcagcaga tgttgaaaca
     4321 acgagcaaac tattgaaaga tttagaagtt attaacccag aggccgatat acaagtcggt
     4381 atgcatggca gcgtcacttt gccaatatca gttagacaaa tgacagcaac ttctgacaat
     4441 aaacataagt ctttacatca aatgattaat catcaatttg tgcaatcacc agtcaaatgt
     4501 actaaagcag agtttataaa acgtttagca tgccttccgt ctcatattta taggttgaaa
     4561 gggtttatga catttgaaga caccgcacat acgtatctca ttcaatttac acaaggacaa
     4621 tatgaattaa cacctgtagc attttcaaaa aaagtgccag aatatttggt gcttatcgga
     4681 aagggtattt caaaagaaga ctatcaatgt ttggaacagt agtgttttca gtggaagaga
     4741 atggttaaca tgccttcatg tataataacg agttgatttg aacgtttaag cgtaaataaa
     4801 aataagcttg gtcagccatc aaatataatt tgaaaactgt ccaagctgtt ttattagaga
     4861 acaatcaatt aaccccacat atttaataat acatcagcaa agccttcagg tttttgaata
     4921 taacctaagt gaccgcctgg aatatctaca ataggtatgc cagtttcttt atttatataa
     4981 aagttaacat cttgtgagaa ggagcctcta gaatctgtcc catttagtag ggtgatttta
     5041 tcgctgtatt ttgtgaaatc atccaaagta atatttgaat gcgtatattg tctaatttca
     5101 aattctaacc agaacatcgt acgtttgtac tgttctatac gtccttcttc agtatcagca
     5161 ggttgagaca tcatttttgc atcaattggt gcgatattta atgtttcgcc aaatgttttc
     5221 atgccttttt ctaagccttc tgttaaaatt tgatgcacaa tgtcatcatt tttatctttc
     5281 caataagtac tgtctggtaa aaatgtatta attggtggtt cgtgaaatgc aatcttttta
     5341 acgacttcag ggtaatcttt taacacatgc atcgcaacga ttgaacctga acttgaacct
     5401 aatatataga caggttcatc acttaatgac tttgcaagtt cggcaatgtc ctgtgcgtcg
     5461 cgtttgacac gataatcact gtcagggttt gaagcggaat cagggagtgg ttcagttaac
     5521 tcgctttctc cataatcacg acgatcaacg gctacaacag taaaatggtc ttttaactgt
     5581 tctgcaagag gcagaaaaat gtctccggta ccgtttgcac caggaataaa gatgagcacg
     5641 ggtccttgtc cgacttggtg gtatcgtaat ttagcgcctt gtaattctaa agtttccata
     5701 ttcaatgacc tccatttgtt aattgttagg tgataaacct aataatttag caccatttgt
     5761 ataacttatt ttctcttttt cttcatctgt taaacccagt tcatctaaaa atacacctaa
     5821 tttttcaggc tcaatatatg gataatcagc agcataaaga attctatcaa tacctacttc
     5881 tttcttgact aaatcaaact gtggcttcgt taacatgcca ctcggtgtga tataaaaatt
     5941 atttttaaag taatagctta cagggtggtt caaatgttca gcgaataaag cttcatccat
     6001 acgttctaag aagaatggga taaactcacc ccaatgtcca ataatcatat ttaactttgg
     6061 ataacgatca aaaataccag ataatactag atgtattgca tgaatgccga catcaatgtg
     6121 ccaaccataa ccaaaacaag caaatgttgc cgcagttact tcaggataat ttcctttata
     6181 gtatgattga taaatgtcac tgttaactgg cgcgggatgt agataaatcg gtacgtctaa
     6241 attttcagct gttttgaaaa taatgtcata tttgtcttga tcaagaaaac catcttgtgc
     6301 acgtcccata atgagcgcac ctttgaatcc taaatcattg atgcaacgtt cgaattctcg
     6361 cgctgcggct tcaggctcat tgataggtaa agttgcaaag cctacaaagc gattgggata
     6421 ttgtgcaata tagtttgcca attgatcatt tgctttttga cataattcaa tggctttctg
     6481 accaaccaaa tttgaaggag aaccatttcc ataagataag acttgaattt gaacgtcttg
     6541 attattcata aattggatac gttcatcatg atgtgataat tcgtcggcat ttgtaaaacc
     6601 tgtctttttt tcaaggcctt ctaacattac tttcatcggt acacctttag gatctgctga
     6661 tatcgcattc atcgtttctt tttgaatatc ttcaatgaca taatgttctt caaacgtaat
     6721 acttttcatt tacttcgcct ccatattgta ttgcatgttt attgcatcta ttgcagaagc
     6781 attttttata tacctctaat ttcaatgttt gtaacataaa attgatctac caaggcatct
     6841 ctccatcgcc attaataaat gtacctgttg ggccatctgc accaatcgtt gctaattgaa
     6901 tgattggctt gattccttca gaaacgtgtt tggaattatt actaaaatca ccaactaaat
     6961 cagtatttgt agcgcctgga tcagcagcat tgatttgcat gttaggtaat cctttagcgt
     7021 attgtagcgt tagcattgtt actgccgatt tagacgaaca ataagctaat gaattcactt
     7081 tagattcagc tgtttcgggg tttgtaacca ttccaaatga acctaaacca cttgatacgt
     7141 tgacgacaac aggttgttca gatttttcta agagagggac gaatgtattc atcattcgta
     7201 cgataccgaa tacattcgtt tgatatactt cttcaacgtc acgaggtgtc aatttggaag
     7261 gtgctgaaaa ttgaccagat atacctgcat tgttaatgag gatatcaaga cggccttctt
     7321 tttcagcaat catgttataa gcatttttga ctgagtagtc acttgtaaca tctaattgta
     7381 cataatgaac acctaatttt tgtgatgctt gttgtcctct tacatcattc cgagaaccta
     7441 tataaacttt gtaacccaat gctttaagtg cctctgcact tgcatagcct aaccctttat
     7501 tgcctcctgt gattaacaca attttagtca ttacgtccca cctcatctaa ataaatgttt
     7561 aataaataat ttctgtacgc ttcaattgaa atatggcgat gctctatttg gaaggcaaat
     7621 acactagttg ataatgattg caacagcata tctgttttga acttcgtgta agtgtcgtca
     7681 tcgcttttaa ataagtcata ataaaaatca aataattctt gataaaatgc gctttggtaa
     7741 aaacgtaatt tattgttgcc tgcttcaata cattgcagta gtgccttatt atcgatttta
     7801 aattgtaaaa gataatctaa cgacacttgc ataacctcat aattagaatg atagtcatct
     7861 ttaatttgct taaaatgagt gataaaaata tcaaggtctc tttgtatgac gtagtagcat
     7921 aaatcgcttt tatctttgaa atgtcgatac aatgtcccca taccgatacc tagttcttta
     7981 gcaatacgat tcatactaat gttttcaacg ccttcttcat caaaaagttt gtgcgctatt
     8041 tcttcaattc gttgcctatt ctcttttgca tcttttcgca tgattacacc tacttaaaat
     8101 tctctaaaat tgacaaacgg ataactctcc gtttattata aaacgtgtta agaaagttag
     8161 caatgaattt gcaataacta ttaaatatca taaaagaaaa gagtgttgat aatgtcttta
     8221 aataaagagc aaagacgcat cacagctgaa gagttgcaag cacatttcga agcatctacc
     8281 ttatcggttc aaatgattgc tgaaaaactg aatgtcacta cagaagatgt ggaaaaagta
     8341 ttagctatga cagcgccact aggcattttt agtcatcaat tacaacgatt tattcattta
     8401 gtatgggatg tcagagatgt aataaacgac aatattaaag gaaatggaca aacaccagaa
     8461 ccatatacgt atttaaaagg tgaaaaagag gactattggt ttttaagata aaaagaggag
     8521 tggatctaaa atgaaagata ttttagtaat aggcgcaaca gggaagcaag gtaatgcagt
     8581 tgtaaaacag ttgttagagg atggatggta tgtaagtgca ttaactagaa ataagaataa
     8641 tcgtaaactt tcagacatag gacatccgca tttatcaatc gttgaaggtg atctgagtga
     8701 taatgttagt ttgcaatcag cgatgaaagg taagtatggc ttatacagta ttcagccaat
     8761 cgttaaagat gatgttagcg aagaattaag acaaggtatg aagataattg aaatagctga
     8821 gcaagaaaat attcaacata ttgtgtatag tactgcggga ggggttaatc gaaatcgaac
     8881 tggcccacac tttgaagtac tagcaaaaat agaaaataga ttaatggaat ctaatataaa
     8941 tgcaacagtt atcaaaccat cattctttat ggataatttt ttacgcattg ctaaagtcga
     9001 agatgaacgt attacattac cagaatttat taatccgaat ataaaattca caatgatttc
     9061 ttctattgat atagctaaaa ttgcatcgta tatttttgca catccacaaa gctttacgca
     9121 tcaatcaata gagattggtt ccgatgaagt tacattaagt gaagcagcaa caatttttag
     9181 tgaagtgaca ggtaaatcta ctgttataga aggagaattt gttagtggtg ttgcagaaaa
     9241 gcaatggttg gaagaaaaag gttatgaagt agactttgaa ttaatggctg aaataaatcc
     9301 aacaagatta tcattaagtg attggcttaa agtccaaaac tataataagt aatgaatggg
     9361 gtgcaatcat gattcaatca atgtggttta atttgcatgt gcaagattta gaaaagagcg
     9421 cacagtttta taaagcgtta ggatttaaaa taaatagaaa cccacaaatg ttagataaaa
     9481 tggtcggcat tcaaataggt caaacaaccg taattttaat agaaaacaag cattttcaaa
     9541 atgtaagtca gcaaagcctt aatactgaac caaatgaagt gatgatttct ctaggtgtga
     9601 acacaaatga agaagttgac cagttggtga ataaagtgaa agaagcgggt ggcacagtcg
     9661 ttcaagaacc aacagtaagc caagggtttt atggagcaat gttcaaagat cttgatggac
     9721 accattttaa ttttttggtc tgctaaaatg aaattctcaa aagaataaat aaaaagccca
     9781 tacagagatg aagcgtaatg tgcaccatgg tgcgacacat tagtgtcttc aatcagtatg
     9841 ggctttgttt atgaggtcag ttggcacctt aagtgcaata cctcgtgagc aaatattaat
     9901 tttgaatata accgtattgt tggcgaagat cataaacttt atctggtgtg acatcctgac
     9961 cgattggatc aaccactttt attgtggaaa atgttgttaa tacttgtcct cggatcatgc
    10021 ttaagtagtc ttgacgcaat gcatgttgtt cttgtttttc agctacagta agtggttgta
    10081 ctttttcttt atttgcaagt tcattaattc tatctaaaat tttcatattg tcatatcctc
    10141 cttagaatca agtgttgata tattttgaga ttatttttat atggtaattt atcaacacat
    10201 taagtaatgt ttttagtttc tcggtctttt gaccagcaga tttttggaat taaaataagg
    10261 gatttgagac acgattttac tagtagtacg atggcaaagt gatgttattg aagtgtcgtt
    10321 tgatatacat gtctgcaatg ttgttttata agacaggatg acgtaactga gcaaagaaat
    10381 gagcattgtc gaatgacatc gtgtgtctta ttaaaattgc aatgggcata gcataggtat
    10441 attcacagtc gattttcttg atttctacat tatcataata cgtagtgtaa aagttatacg
    10501 acagtcgata gtcgattcgc tttttaccac gagcaattat tatgtgttgc ttcaacgtcg
    10561 actttaattt atttgtaatg tatgtcacta cacccagact actctgatta atatttaata
    10621 tattatgcgt gcgttcaaac ccctgaggtt catctgtctc gctcacgtca atccctccta
    10681 tcttagtatt taaacaacgc atatcaacta tgcaacatat gttgtttaga catactatac
    10741 tactatgtcg tacgtttgcc aataaacacg cacatatcat ttgttgccta aacaacatat
    10801 tgcctgaagt gttgtttaag tacgcaaaaa caaaaatgct tgtcactaaa atctatatgt
    10861 tttaatgaca agcttatttt cattcagtta acgactgcta atatgacatt gtagagtata
    10921 ggacgttgat ttatgtccta gacacggcta atcaatcgta atacgttaaa ttttattgtg
    10981 tgtacaagta tgaacctaag tcacatagtg atttcaagat gcatgactaa ataccaagtt
    11041 ttattaaaaa tgtgctatat gatgaattaa aaatggaagg ttgcgatcga taaacacatc
    11101 ggctgtctct tgactcgtgt taaaccaatg taatgctaag cctgagatac cagctgctaa
    11161 aaaactcgca ctatttacaa tgatatctcg ttgatgctcc ggatagctgt tttctaacat
    11221 aatcgcaaaa atatcttcta attcattatt aatacgttga tgtgctttgt tgcaaaatgc
    11281 ttcactattt aattcgcaag actttgcagc atctttgata taactcgcaa ttgaaatgaa
    11341 aatattacgc agaacctttt cattaatgac attagaaatg ctcaaattat cattcaagtc
    11401 ttttaaaatg gttacagata atgtgtagtc taggaggtct tctttatcag tgaaatgagc
    11461 gtaaaatgtt gcacgattta gcgtagcaat atcagtgata tctttaactg taatttgaga
    11521 taatttcttt tctctagaaa ttttaagaaa agcatcgaca agcaattgtt tggttctaac
    11581 gattcttgga tctatggatt tactttccat catttgttca ctacctaaca tctgttgttt
    11641 taacacttat tatcagtatc ttctattata ttgtcaattg tgttgatgat atgtaagtgc
    11701 tgatacgctc ctaacaaata atgtaccttt ttggtaagca tttgaatgtc tttcttctat
    11761 tgtattaatg gattttaaaa atgaagataa aataaaagtg tattgtaaca atatgtttga
    11821 aaaattgcca atttctttat ctgaaaattc agaattttgt gatatactat ttttcatagt
    11881 cttgagttat agatgatact gctagcctaa atataacaac atcggcatgc acatcttaga
    11941 atttaagcta gtggcagctt ataacttaag gctctttatt atttgcatac agatgtatat
    12001 acataaatgg aaggatgata ttatgacatc aattaaaaca attacaccca aagattttat
    12061 atttagagtt ttatcaggtg tcgccatagg tattgttgcg ggtttagttc ctaatgcgat
    12121 acttggagaa atttttaaat actttatgga ttatcatccg atttttaaaa cgttattagg
    12181 cgttgtcgtt gcaattcaat ttacagtacc tgctttgatt ggtgcattgg ttgccatgaa
    12241 atttgaccta tcgccattag caattgctgt tgtggcaagt gcagcatatg taggaagcgg
    12301 tgcagcgcag tttaaaaatg gtgcttggat gattacgggt attggtgatt taattaatac
    12361 aatgattaca gcagcgattg cagtgttatt cattttactt attcaacatc gcgtaggtag
    12421 tatggcattg attgtattcc caacagttgt tggcggtatt tcaggtgcaa ttggtgtttt
    12481 aatattgccg tatacgaaaa tgattacaac agccatcggt aatatggtta atggttttac
    12541 agaattacaa ccgattgtca tgagtatttt gatttcaatg gtatttagtc ttattattat
    12601 ttcgccacta tcaactgtgg ccattgcctt tgcgataggt attacaggtt tagcagctgg
    12661 atcagcgtca attggtatct ccgcgactga agcagtattg ataattggta caagtaaagt
    12721 gaatcgttta ggggtgccgt tatccgtctt ctttggaggc gttaaaatga tgatacctaa
    12781 tatggtgaaa tatccaattc ttatgttacc aatattaacg acagccattg tatcaggttt
    12841 ggtatcagca ttagtaggta ttcatggaac gaaagagtca gctggatttg gttttatagg
    12901 aatggtagga ccgattaatg catttaaatt tatggaggtt gattctgctt ggttgagtgt
    12961 cttactaatt gtagtagcgt tctttgttgt accatttgta acagcttggc ttgcagatat
    13021 catttatcgt aaagtgtttc gtttatatac taatgatatt tttaaattca tgggataata
    13081 acagtatgtt ttttgaaaat atgagaccaa aaattattta atgttattgt gaattgcgag
    13141 aatgaagttg aacaatataa ttgagctgca gtttatttgc gctaataaaa gataagacaa
    13201 ttcacaaaag atgtgtgttt taaaactttg aatatacaac aataatagca atcaatacat
    13261 gtgtagaaac atatttgttg attgcttttt aattgcgttt aatgtacgaa aataagagaa
    13321 gttacatgtc agatatatta cagctatgta acaaaaatac aatctgtaat attacgaaag
    13381 ctgtaatagt tgagctaaat atagtaaaaa tcttatgtgt taaagactat ttcaagggtt
    13441 gattggattc taaagggcac atatttcaca ttacagtata aaacagccat atttcaacat
    13501 tacaaataac acttgatatt gtaatgtttt gtaaagaaag tgtaatttac tggctggttt
    13561 tttgtgatat agtgagtacc gttgaaagaa ataaaagaca atttaaactt gcactacgac
    13621 attcaaattc aaaatataat tactaaaaac ataaacttac aattattact ggaggatttt
    13681 ttactatgaa aaagacaatt atggcatcat cattagcagt ggcattaggt gtaacaggtt
    13741 acgcagcagg tacaggacat caagcacacg ctgctgaagt aaacgttgat caagcacact
    13801 tagttgactt agcgcataat caccaagatc aattaaatgc agctccaatc aaagatggtg
    13861 catatgacat ccactttgta aaagatggtt tccaatataa cttcacttca aatggtacta
    13921 catggtcatg gagctatgaa gcagctaatg gtcaaactgc tggtttctca aacgttgcag
    13981 gtgcagacta cactacttca tacaaccaag gttcaaatgt acaatcagta agctacaatg
    14041 cacaatcaag taactcaaac gttgaagctg tttcagctcc aacttaccat aactacagca
    14101 cttcaactac ttcaagttca gtgagattaa gcaatggtaa tactgcaggt gctactggtt
    14161 catcagcagc tcaaatcatg gctcaacgta ctggtgtttc agcttctaca tgggctgcaa
    14221 tcatcgctcg tgaatcaaat ggtcaagtaa atgcttacaa cccatcaggt gcttcaggtt
    14281 tattccaaac tatgccaggt tggggtccaa caaacactgt tgaccaacaa atcaacgcag
    14341 ctgttaaagc atacaaagca caaggtttag gtgcttgggg attctaattc cttcataata
    14401 aataaaagta atgtttagac attaaatata tagctcttcc catagaaatt ggggagagtt
    14461 tttttattaa ttaaaaatac attttttaaa aagtggatat tgaagttagg gaaatttatt
    14521 ggactttatt gtattactgc taaaatactt gtctttatta agtgccaagt ggaatgaaac
    14581 ttcatttgag gctgtactga gtacactaga tacaaactaa acgcacattt tataattaaa
    14641 ttaaatgaaa aaggagataa aaatggctat aaacataatt gaatataata ggtcttataa
    14701 ggaagagcta atcgagttta ttttatcgat tcaaaaaaat gaatttaata taaaaattga
    14761 tagagatgat caacctgatt tggagaacat cgaacataac tatttgaata atggagggca
    14821 attttggctt gcaataaaca atcatcaaaa tattgttggt actataggtt tgattagact
    14881 agataataat atgtctgcac ttaaaaaaat gtttgttgat aaaggttata ggaatttaaa
    14941 aataggtaaa aaacttttag ataaagttat aatgacatgt aaagaacaaa atatcgatgg
    15001 tatttattta ggaacaattg ataaatttat tagtgcacag tatttttatt ctaataatgg
    15061 cttccgtgaa attaagcgag gagatttacc aagtagtttt cctaaattag atgtagataa
    15121 taggttttat tacagaaatt taaaagatta attcg
//
LOCUS       DAKTIO010000053        14971 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.053, whole genome shotgun sequence.
ACCESSION   DAKTIO010000053 DAKTIO010000000
VERSION     DAKTIO010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14971
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.053"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            63..821
                     /gene="nagB"
                     /locus_tag="O0293_001239"
     CDS             63..821
                     /gene="nagB"
                     /locus_tag="O0293_001239"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCY0280128.1"
                     /translation="MKVLNLGSKKQASFYVACELYKEMAFNQHCKLGLATGGTMTDLY
                     EQLVKLLNKNQLNVDNVSTFNLDEYVGLTASHPQSYHYYMDDMLFKQYPYFNRKNIHI
                     PNGDADDMNAEASKYNDVLEQQGQRDIQILGIGENGHIGFNEPGTPFDSVTHIVDLTE
                     STIKANSRYFKNEDDVPKQAISMGLANILQAKRIILLAFGEKKRAAITHLLNQEISVD
                     VPATLLHKHPNVEIYLDDEACPKNVAKIHVDEMD"
     gene            898..1530
                     /gene="hxlA"
                     /locus_tag="O0293_001240"
     CDS             898..1530
                     /gene="hxlA"
                     /locus_tag="O0293_001240"
                     /EC_number="4.1.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hexulose-6-phosphate synthase"
                     /protein_id="HCY0280129.1"
                     /translation="MELQLAIDLLNKEDAAELANKVKDYVDIVEIGTPIIYNEGLPAV
                     KHMADNISNVKVLADMKIIDAADYEVSQAIKFGADVITILGVAEDASIKAAIEEAHKN
                     NKQLLVDMIAVQDLEKRAKELDEMGADYIAVHTGYDLQAEGQSPLESLRTVKSVIKNS
                     KVAVAGGIKPDTIKDIVAESPDLVIVGGGIANADDPVEAAKQCRAAIEGK"
     gene            1532..2080
                     /gene="hxlB"
                     /locus_tag="O0293_001241"
     CDS             1532..2080
                     /gene="hxlB"
                     /locus_tag="O0293_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HCY0280130.1"
                     /translation="MAKFSDYQLILDELKMTLSHVEADEFSTFASKILHAEHIFVAGK
                     GRSGFVANSFAMRLNQLGKQAHVVGESTTPAIKSNDVFVIISGSGSTEHLRLLADKAK
                     SVGADIVLITTNKDSAIGNLAGTNIVLPAGTKYDEQGSAQPLGSLFEQASQLFLDSVV
                     MGLMTEMNVTEQTMQQNHANLE"
     gene            2193..2840
                     /locus_tag="O0293_001242"
     CDS             2193..2840
                     /locus_tag="O0293_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCY0280131.1"
                     /translation="MKFDNYIFDFDGTLADTKKCGEVATQSAFKACGLTEPSSKEITH
                     YMGIPIEESFLKLADRPLDEAALAKLIDTFRHTYQSIEKDYIYEFAGITEAITSLYNQ
                     GKKLFVVSSKKSDVLERNLSAIGLNHLITEAVGSDQVSAYKPNPEGIHTIVQRYNLNS
                     QQTVYIGDSTFDVEMAQRAGMQSAAVTWGAHDARSLLHSNPDFIINDPSEINTVL"
     gene            3342..4742
                     /locus_tag="O0293_001243"
     CDS             3342..4742
                     /locus_tag="O0293_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0280132.1"
                     /translation="MDFNKENINMVDAKKAKKTVVATGIGNAMEWFDFGVYAYTTAYI
                     GANFFSPVENADIRQMLTFAALAIAFLLRPIGGVVFGIIGDKYGRKVVLTSTIILMAF
                     STLTIGLLPSYDQIGLWAPILLLLARVLQGFSTGGEYAGAMTYVAESSPDKRRNSLGS
                     GLEIGTLSGYIAASIMIAVLTFFLTDEQMASFGWRIPFLLGLFLGLFGLYLRRKLEES
                     PVFENDVATQPERDNINFLQIIRFYYKDIFVCFVAVVFFNVTNYMVTAYLPTYLEQVI
                     KLDATTTSVLITCVMAIMIPLALMFGKLADKIGEKKVFLIGTGGLTLFSIIAFMLLHS
                     QSFVVIVIGIFILGFFLSTYEATMPGSLPTMFYSHIRYRTLSVTFNISVSIFGGTTPL
                     VATWLVTKTGDPLAPAYYLTAISVIGFLVITFLHLSTAGKSLKGSYPNVDNEQDRAYY
                     AEHPKEALWWVKERKN"
     gene            5286..6662
                     /locus_tag="O0293_001244"
     CDS             5286..6662
                     /locus_tag="O0293_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HCY0280133.1"
                     /translation="MNVILEQLKTHTQNKPNDIALHIDDETITYSQLNARITSAVESL
                     QKYSLNPVVAINMKSPVQSIICYLALHRLHKVPMMMEGKWQSTIHRQLIEKYGIKDVI
                     GDTGLMQNIDSPMFIDSTQLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEM
                     LMLKNENAIAAPGPLSHSLTLYALLFALSSGRTFIGQTTFHPEKLLNQCHKISSYKVA
                     MFLVPTMIKSLLLVYNNEHTIQSFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETS
                     FISYNLNQQAPVESVGVLFPNVELKTTNHDHNGIGTICIKSNMMFSGYVSEQCINNDE
                     WFVTNDNGYVKEQYLYLTGRQQDMLIIGGQNIYPAHVERLLTQSSSIDEAIIIGIPNE
                     RFGQIGVLLYSGDVTLTHKNVKQFLKKKVKRYEIPSMIHHVEKMYYTASGKIAREKMM
                     SMYLRGEL"
     gene            6664..7803
                     /locus_tag="O0293_001245"
     CDS             6664..7803
                     /locus_tag="O0293_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HCY0280134.1"
                     /translation="MNQAVIVAAKRTAFGKYGGTLKHLEPEQLLKPLFQHFKEKYPEV
                     ISKIDDVVLGNVVGNGGNIARKALLEAGLKDSIPGVTIDRQCGSGLESVQYACRMIQA
                     GAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENVA
                     KMYDVSRELQDEFAYRSHQLTAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDN
                     FGRFKPVIKGGTVTAANSCMKNDGAVLLLIMEKDMAYELGFEHGLLFKDGVTVGVDSN
                     FPGIGPVPAISNLLKRNQLTIENIEVIEINEAFSAQVVACQQALNISNTQLNIWGGAL
                     ASGHPYGASGAQLVTRLFYMFDKETMIASMGIGGGLGNAALFTRF"
     gene            7778..8143
                     /locus_tag="O0293_001246"
     CDS             7778..8143
                     /locus_tag="O0293_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraC"
                     /protein_id="HCY0280135.1"
                     /translation="MQHYLLDSNQRLNVSFSKDSVAAYYQCFNQPYRKEVPPLMCASL
                     WPKFDLFKKYANSELILTKSAINQTQKIEVDTIYVGHLEDIECRQTRNITRYTMALTL
                     TKNDQHVITVTQTFIKAMK"
     gene            8146..8415
                     /locus_tag="O0293_001247"
     CDS             8146..8415
                     /locus_tag="O0293_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280136.1"
                     /translation="MKFNEIWINEYLALVNDDNPIHNEIVPGQLVSQMMLMAMSLETN
                     QCQINYVKPILINENIEFIEQHEHEIIAINDDGEIKIKISLSTKK"
     gene            complement(8616..8783)
                     /locus_tag="O0293_001248"
     CDS             complement(8616..8783)
                     /locus_tag="O0293_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraX"
                     /protein_id="HCY0280137.1"
                     /translation="MIIYRQYHHEGAPVYEIITKTFQHVSIKCDDSFSDTEIFKLLSL
                     LQDDIDHMKVS"
     gene            complement(9290..10120)
                     /gene="thiD"
                     /locus_tag="O0293_001249"
     CDS             complement(9290..10120)
                     /gene="thiD"
                     /locus_tag="O0293_001249"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCY0280138.1"
                     /translation="MALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTM
                     DKDTWSHDVTPLPMDVFEKQLETALSIGPDAIKTGMLGTEEIIKRAGEVYEASNAQYF
                     VVDPVMVCKGEDEVLNPGNTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKK
                     AATIIFDKGAQHVIIKGGKALDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAA
                     ATTAYLANGKSPKEAVISAKAFVASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV
                     "
     gene            10304..10960
                     /locus_tag="O0293_001250"
     CDS             10304..10960
                     /locus_tag="O0293_001250"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCY0280139.1"
                     /translation="MEWSQIFHDITTKHDFKAMHDFLEKEYSTAIVYPDRENIYQAFD
                     LTPFENIKVVILGQDPYHGPNQAHGLAFSVQPNAKFPPSLRNMYKELADDIGCVRQTP
                     HLQDWAREGVLLLNTVLTVRQGEANSHRDIGWETFTDEIIKAVSDYKEHVVFILWGKP
                     AQQKIKLIDTSKHCIIKSVHPSPLSAYRGFFGSKPYSKANAYLESVGKSPINWCESEA
                     "
     gene            10961..11341
                     /locus_tag="O0293_001251"
     CDS             10961..11341
                     /locus_tag="O0293_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5327 family protein"
                     /protein_id="HCY0280140.1"
                     /translation="MLNRETLIARIEQELVQAEQAQHDHDFEKHMYAIHILTSLYAST
                     SNTPHIGEQQMNRRIANHNQMPQSQITQPTHQVTVAEIEAMGGKVNTHSAHHHNKSYS
                     QPSNQQQRLATDDDIGNGESIFDF"
     gene            11474..11842
                     /locus_tag="O0293_001252"
     CDS             11474..11842
                     /locus_tag="O0293_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCY0280141.1"
                     /translation="MKLFIILGALNAMMAVGTGAFGAHGLQGKISDHYLSVWEKATTY
                     QMYHGLALLIIGVISGTTSINVNWAGWLIFAGIIFFSGSLYILVLTQIKVLGAITPIG
                     GVLFIIGWIMLIIATFKFAG"
     gene            11963..13447
                     /locus_tag="O0293_001253"
     CDS             11963..13447
                     /locus_tag="O0293_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCY0280142.1"
                     /translation="MEKKNKQIDRGDLKQNLSEKFVWAIAYGSCIGWGAFILPGDWIK
                     QSGPIAASIGIVIGALLMILIAVSYGALVERFPVSGGAFAFSFLSFGRYVSFFSSWFL
                     TFGYVCVVALNATAFSLLVKFLLPDVLNNGKLYTIAGWDVYITEIIIATVLLLVFMLV
                     TIRGASVSGSLQYYFCVAMVIVVLLMFFGSFFGNNFALENLQPLAEPSKGWLVSIVVI
                     VSVAPWAYVGFDNIPQTAEEFNFAPNKTFKLIVYSLLAASLTYVVMILYTGWLSTSHQ
                     SLNGQLWLTGAVTQTAFGYIGLGVLAIAIMMGIFTGLNGFLMSSSRLLFSMGRSGIMP
                     TMFSKLHSKYKTPYVAIIFLVGVSLIAPWLGRTALTWIVDMSSTGVSIAYFITCLSAA
                     KLFSYNKQSNTYAPVYKTFAIIGSFVSFIFLALLLVPGSPAALTAPSYIALLGWLIIG
                     LIFFVIRYPKLKNMDNDELSRLILNRSENEVDDMIEEPEKEKTK"
     gene            complement(13589..14041)
                     /locus_tag="O0293_001254"
     CDS             complement(13589..14041)
                     /locus_tag="O0293_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCY0280143.1"
                     /translation="MFWILNFIFSFLASMFFCVIFDAPRKLYLSCGFVGTCGWMVYTL
                     FFNGFNVHTIYSSFFGSLALGLLSHYMARKQKEPAIIFMVTGIIPLVPGGLAYDATKN
                     LVLLNFSTAINTMLEVTLIAGAIALGLLFADQISKLIVSGFVKSFKRL"
     gene            complement(14054..14818)
                     /locus_tag="O0293_001255"
     CDS             complement(14054..14818)
                     /locus_tag="O0293_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCY0280144.1"
                     /translation="MDINSEEYKQEVLIKDVVMLAARILLESGAEGTRVEDTMTRIAK
                     KLGYSESNSFVTNTVIQFTLHSESFPRIFRITSRDTNLIKISQANKISRQITNNEISL
                     AEAKTQLEKIYVAKRDSSLPFKGFAAAMIAMSFLYLQGGRLIDVLTAILAGSLGYLVT
                     EILDRKLHAQFIPEFIGSLVIGIIAVIGHTLIPTGDLATIIIAAVMPIVPGVLITNAI
                     QDLFGGHMLMFTTKSLEALVTAFGIGAGVGSVLILV"
BASE COUNT         5152 a         2251 c         2752 g         4816 t
ORIGIN      
        1 atggtgtatg aatgaattca taagttttta aaatgtatta aatttgtgga ggcatgtaaa
       61 caatgaaagt attaaactta ggatcgaaaa aacaagcatc attctatgtt gcatgtgagt
      121 tatataaaga gatggcattt aatcagcact gtaaactagg tttagcaact ggtggtacaa
      181 tgacagattt gtatgaacaa cttgttaagt tattaaataa aaatcagtta aacgtagaca
      241 atgtatccac gtttaattta gacgaatatg taggtttaac cgcatcacat ccgcaaagtt
      301 atcactatta tatggatgac atgcttttca aacaatatcc ttattttaat agaaagaaca
      361 ttcatattcc aaatggagat gccgatgata tgaatgcgga agcgtcaaaa tataatgacg
      421 ttttagaaca acaaggtcaa cgtgatattc aaattttagg tattggtgaa aatggtcata
      481 ttggatttaa tgaacctggt acgccgtttg atagcgttac tcatatcgtt gatttgactg
      541 aaagtactat taaggctaat agtcgatatt ttaaaaacga agatgatgtt ccaaagcaag
      601 ccatttcgat gggacttgct aatattcttc aagccaaacg tatcatttta ctcgcatttg
      661 gtgaaaagaa acgtgctgct attacacatt tattaaatca ggaaatttct gttgatgttc
      721 cagccacatt acttcacaaa cacccgaatg ttgagatata tttagacgac gaagcttgcc
      781 cgaaaaatgt tgcgaaaatt catgtcgatg aaatggattg attgcaatgt ttaattaaga
      841 aatgcctcgg gaaaggttcc aatagaaaga taaaaagcat tggaaggatg atttttagtg
      901 gaattacaat tagcaattga tttattaaac aaagaagacg cggctgagtt agcaaataaa
      961 gtaaaagatt atgtagatat cgtagaaatc ggtacgccaa tcatttacaa cgaaggttta
     1021 ccagcagtta aacatatggc agacaacatt agtaatgtaa aagtattagc agacatgaaa
     1081 attatagatg cagctgatta tgaagttagc caagcaatta aatttggcgc ggatgtaatt
     1141 acaatactag gtgttgcaga agatgcatca attaaagcag ctattgaaga agctcataaa
     1201 aataataaac aattactagt tgatatgatt gctgttcaag atttagaaaa acgtgcaaaa
     1261 gaactagatg aaatgggtgc tgattatatt gcagtacaca ctggttatga tttacaagca
     1321 gaagggcaat caccattaga aagtttaaga accgttaaat ctgttattaa aaattctaaa
     1381 gttgcagtag caggtggaat taaaccagat acaattaaag atattgtcgc tgaaagtcct
     1441 gatcttgtta ttgttggtgg cggaatcgca aatgcagatg atccagtaga agctgcaaaa
     1501 caatgtcgcg ctgcaatcga aggtaagtaa tatggctaaa tttagtgact atcaattaat
     1561 tctagatgaa ttaaagatga ctttgtcaca tgttgaagcg gatgagtttt caacttttgc
     1621 atccaaaata ctacatgctg aacatatatt tgtagctggc aaaggacgtt caggattcgt
     1681 ggcgaatagt tttgcaatgc gcttaaatca gctcggcaaa caggcacatg ttgttggaga
     1741 atcaacgaca cctgcgatta agtcgaatga tgtatttgta attatctctg gttcaggttc
     1801 cacggaacat ttaagattat tagcagacaa agcaaaatca gtaggtgctg acatcgtatt
     1861 aattactaca aataaagatt ctgcaatagg caatctagct gggacgaaca tcgttttgcc
     1921 tgcaggtaca aaatatgatg aacaaggctc ggcacaacca ttaggaagtt tgtttgaaca
     1981 agcatctcaa ttatttttag atagtgttgt aatgggattg atgactgaaa tgaatgttac
     2041 ggaacaaacg atgcaacaaa atcatgctaa tttagaataa aataaagata gtcgataata
     2101 tgatgcctag gcagaaatat tatcgattat ttttttattt aaataataaa ttatagtata
     2161 atatcaataa taaacgaata ggggtgttaa tattgaagtt tgacaattat atttttgatt
     2221 ttgatggtac gttggcagac acgaaaaaat gtggtgaagt agcaacacaa agtgcattta
     2281 aagcatgtgg cttaacggaa ccatcatcta aagaaataac gcattatatg ggaataccta
     2341 ttgaagaatc atttttaaaa ttagcagacc gaccattaga tgaagcagca ttagcaaagt
     2401 taatcgatac atttagacat acatatcaat ctattgaaaa ggactatatt tatgaatttg
     2461 cgggtataac tgaagccatt acaagtttgt ataaccaagg gaaaaaactt ttcgtggtgt
     2521 ctagtaagaa gagtgatgta ttagaaagaa atttatcggc tattggatta aatcacttga
     2581 ttaccgaagc tgttggatcc gatcaagtaa gtgcatataa accaaatcct gaaggcatac
     2641 acacaattgt gcaacgctac aatttaaata gccaacaaac ggtgtatatt ggtgattcaa
     2701 cgtttgatgt tgagatggca caacgtgctg gtatgcaatc tgcagctgtc acttggggtg
     2761 cacatgatgc aaggtcatta cttcattcaa atccggattt tattattaat gatccatcag
     2821 aaattaatac cgtattataa aacttgttaa aacagagaat accatggtta aaatgcatat
     2881 tcataaatat tagattatac ttagaaatat ttcgctttag attaggaatt taaaataaat
     2941 atttattaaa cattatgaat ttttaaagag taatgtctga ctcgttgata atttattttt
     3001 gtaaaaataa attaaagtaa tgacaaagtt attgaagtaa attgagtata aacatttaaa
     3061 tacgatgtcg aaaatggcga tagcatatca cttacatgaa gttatgtgct atcgctattt
     3121 ttagttataa ttccaaaaag ttaatcgttc gatgatttaa gaattattat tgtttaattc
     3181 aaatgtatga gggtataaaa tcattgaatt taattcgata aagcgaaatt tttgaacaaa
     3241 catacttttg tatttatata aaagtttaaa ttcttataaa tttgacaaaa ctaattaact
     3301 ccgtataatt atgaaacata caagagggag tgtatgaatt catggatttt aataaagaga
     3361 atattaacat ggtggatgca aagaaagcta aaaaaaccgt tgttgcaacc ggtatcggta
     3421 atgcaatgga atggttcgat tttggtgtct atgcatatac aactgcgtac attggagcga
     3481 acttcttctc tccagtagag aatgcagaca ttcgacaaat gttgactttc gcagcattag
     3541 ccattgcgtt tttattaaga ccaattggtg gtgtcgtatt tggtattatt ggtgacaaat
     3601 atggacgtaa agttgtatta acatctacaa ttattttaat ggcattttca acattaacca
     3661 ttggattatt gccaagctat gatcaaattg gactttgggc accaatacta ttattgcttg
     3721 caagagtact acaagggttt tcaacaggtg gagagtatgc gggggcaatg acatatgttg
     3781 ccgaatcatc tccagataag cgtcgtaact cattaggtag tggactagaa attgggacat
     3841 tatcaggtta catagctgct tcaattatga ttgctgtatt aacattcttt ttaacagatg
     3901 aacaaatggc atcatttggt tggagaatcc cattcttact cggtttattc ctaggattat
     3961 tcggcttata tttacgtcgt aagctggaag aatcaccagt tttcgaaaat gatgttgcaa
     4021 cacaaccaga aagagataac attaactttt tacaaatcat cagattttat tacaaagata
     4081 tatttgtatg ttttgtagct gttgtattct tcaatgttac aaactatatg gtaactgcat
     4141 atttaccaac ctatttagaa caagttatta aattagatgc aacgacaaca agtgtattaa
     4201 ttacttgtgt catggcaata atgattccat tagcattaat gtttggtaag ttagcggata
     4261 aaataggtga aaagaaagta tttctaattg gtactggtgg gctaacatta ttcagtatca
     4321 tcgcatttat gttattacat tcacaatcat ttgttgtaat agtaatcggt atatttatat
     4381 taggattttt cttatcaact tacgaagcga caatgccagg gtcgttacca acgatgtttt
     4441 acagtcatat aagatatcga actttatcag taacatttaa tatctctgtt tcgatatttg
     4501 gtggtacgac gccattagtt gcaacatggt tagttacgaa aactggagat ccattagcac
     4561 ctgcgtatta tttaacagca atcagtgtta ttggcttttt agttattaca ttcttacatt
     4621 taagtacagc aggaaaatct ctaaaaggtt cgtatccaaa tgtagataac gagcaagata
     4681 gagcttatta tgcagaacat ccaaaagaag cattatggtg ggttaaagaa cgtaagaatt
     4741 agagatttta ataaaaagta taaatcaatc gtatataagc actttaaagc tagtaggttc
     4801 tgctaacttt aaagtgcttt ttaaattgag aactgtaatt agccgtaata aagtttttgt
     4861 atatacataa acccccactg caatgattat cgcaatgggg gaaagagggg acttaaagca
     4921 tatgtttagc tttgaatact taaaattctc ttgctattga aatgttagga tgtaaatatg
     4981 tcttagagta ttttgtccaa cgcaattaat attgagactc taaccttcaa tattattata
     5041 gagaacacaa acttaaatag attgggtgac ttatttgtgt cagttattgc gattgcgata
     5101 acttcttttc tctatataca tatagtaacg tcttatctaa taaaaaacat ggtactacag
     5161 tatcaaattt atctagggct taagtttgat ttttataata ggcaggttta cctgataaaa
     5221 atacttattc attatataat gttaacaata tgtattttaa agtttacatt gagtgaggga
     5281 tattgatgaa cgtaatttta gaacagttga aaacacatac tcaaaataaa cctaatgaca
     5341 tagcattaca tatcgatgat gaaacaatta catatagtca actaaatgcc cgcatcacta
     5401 gcgcagttga atctttgcag aaatattcac ttaaccctgt cgttgctatt aatatgaaat
     5461 caccggtgca aagtattatt tgttatttag ctttgcatcg tttacataaa gtgcctatga
     5521 tgatggaagg taaatggcaa agtactatac atcgtcaatt gattgaaaaa tatggtatta
     5581 aagatgtaat tggagataca ggtctcatgc agaatataga ctcaccgatg tttattgatt
     5641 caacgcaatt acagcactac cccaatttat tacatattgg ttttacttca gggacaactg
     5701 gactgccaaa agcatattat cgtgatgaag attcatggtt ggcttctttt gaagttaatg
     5761 aaatgttgat gttaaaaaat gaaaatgcaa tagcagcccc tggaccacta tcgcactcgt
     5821 taacattata tgcgttattg tttgctttaa gttccggtcg tacttttata ggacagacca
     5881 cttttcatcc tgaaaagtta cttaatcaat gtcataaaat atcatcatac aaagttgcta
     5941 tgtttcttgt tccaacgatg attaaatcat tattgttagt ttacaacaat gaacatacaa
     6001 tccaatcatt ttttagcagt ggagataagc tgcattcttc tatttttaaa aagataaaaa
     6061 atcaagcaaa tgacataaat ttgattgaat tttttggtac atcggaaacc agttttatca
     6121 gctataactt gaatcagcaa gcaccagttg aatcagtagg tgtgctattt ccaaatgtgg
     6181 aattgaaaac aacgaatcac gatcacaatg gtataggaac tatttgtata aaaagtaata
     6241 tgatgtttag tggctatgta agtgaacaat gtataaataa tgatgaatgg tttgttacta
     6301 atgataatgg ctatgtaaaa gagcagtatt tatatttaac gggacgtcaa caggatatgt
     6361 taattattgg tggtcaaaat atatatccag cacatgttga acgcctttta acgcaatctt
     6421 cgagcattga tgaagcaatt atcatcggta ttccaaatga gcgttttggt caaataggcg
     6481 tattgcttta ttctggtgat gtgacactta cacataaaaa tgtaaaacaa tttttaaaaa
     6541 agaaagtgaa acgctatgaa attccatcga tgattcatca tgtagaaaag atgtattaca
     6601 ctgcaagtgg taaaattgct agagaaaaaa tgatgtcgat gtatttgaga ggtgaattat
     6661 aatatgaatc aagcagtcat agttgcagct aaacgaactg catttgggaa atatggtggc
     6721 actttaaaac atttagagcc agaacaattg cttaaacctt tattccaaca ttttaaagag
     6781 aagtatccag aggtaatatc taaaatagat gatgtagttt taggtaatgt tgttgggaat
     6841 ggtggcaata ttgcaagaaa agcattgctt gaagcggggc ttaaagattc aatacctggc
     6901 gtcacaatcg atcggcaatg tgggtctgga cttgaaagtg ttcaatatgc atgtcgcatg
     6961 atccaagccg gagctggcaa ggtatatatt gcaggtggtg ttgaaagtac aagtcgagca
     7021 ccttggaaaa tcaaacgacc gcattctgtg tacgaaacag cattacctga gttttatgag
     7081 cgtgcatcat ttgcacctga aatgagcgac ccatcaatga ttcaaggtgc tgaaaatgtg
     7141 gccaagatgt atgatgtttc aagagaatta caagatgaat ttgcttatcg aagtcatcaa
     7201 ttgacagcgg aaaatgtaaa aaatggaaat atttctcagg aaatattacc tataaccgtt
     7261 aaaggagaaa tattcaacac tgatgaaagt ctaaaatcac atattccgaa agataacttt
     7321 ggccgattta agcccgtgat caaaggtggg accgttaccg ctgcgaatag ttgtatgaaa
     7381 aatgatggtg cagttttatt gcttattatg gaaaaagata tggcatacga attaggtttc
     7441 gagcatggtt tattatttaa agatggtgtt acggtaggtg ttgattctaa ttttcctggc
     7501 attggtccag taccagccat ttccaactta ctaaaaagaa atcaattaac gatagaaaat
     7561 attgaagtca ttgaaattaa cgaagcgttc agtgcacagg tagttgcctg ccaacaagct
     7621 ttaaatattt caaatacgca attaaatata tggggtggtg cattagcatc aggtcatcca
     7681 tacggtgcaa gcggtgccca attagtgact cgattatttt atatgtttga caaagagact
     7741 atgattgcat ctatggggat agggggaggt ctaggaaatg cagcattatt tactcgattc
     7801 taaccagcga ttaaatgtgt cattttctaa ggatagtgtg gctgcatatt atcagtgttt
     7861 taaccaacct tatagaaaag aagtaccacc attaatgtgt gcgtcattat ggccaaaatt
     7921 tgatttattt aaaaaatatg caaatagcga actgatttta acaaaatcag caattaatca
     7981 aactcaaaag atagaagtag acacaatata tgtagggcat ttagaagata ttgaatgccg
     8041 acagactcgc aatatcacac gttatacaat ggctttaaca ttaactaaaa atgatcaaca
     8101 tgtcataacg gttacacaaa cttttattaa ggcgatgaag tagagatgaa gtttaatgag
     8161 atatggataa atgaatattt ggcgctcgta aatgatgata atccaataca taatgagatt
     8221 gtgccaggac aattagtgag tcaaatgatg ctgatggcta tgtcattaga gacaaaccag
     8281 tgtcaaatta actacgttaa acctatttta ataaatgaaa atatcgaatt cattgaacaa
     8341 cacgaacacg aaattatagc aattaatgac gatggagaga ttaaaataaa aatttctttg
     8401 agcacaaaaa aataaccgat attagctgca tgaacgcata ttaattagga gatgaaagga
     8461 cagctaatat cagttatgta ttgttattat tattgggaac agagatgaat ataggttacg
     8521 tttctttctt tgcacgggga tgcattaatc taaaataata ataacaacta tatcaatgtt
     8581 taataaattc tggattattg gaacgattag tcaatttaac taactttcat atgatctata
     8641 tcgtcttgta ataaagagag caatttgaat atttcagtat cactaaatga atcgtcacat
     8701 ttaattgaaa catgctgaaa cgttttggtt ataatttcat aaactggtgc gccttcatgg
     8761 tgatactgtc gataaataat cataacctat attacctcct ttgctactct atggttatat
     8821 tataaataac atttttatgt gtgacatcaa ccttaagtat caacttttta tcagacatag
     8881 aacgtatgat ttactaagac tatttatgta taaaagttct aaataaatat atatttatag
     8941 agtcgcctgg cagtcatttg ggaaatataa catatatgat tagagaggca tctatcgcaa
     9001 aagaatgata atgatagagg tattgagcat atagatgagt ttaagttcat cttgaaaata
     9061 aagggttatt tagtcataga tgtagatgta taggaaatat ttgtatgtat tgttcgatat
     9121 gtatgaaatt ttcaataaaa gctaataacg cttatatgta actttcaaat ttaaattata
     9181 tacagagcat gatgattata aaaaaataac cacatcacat aaattgagtt catacccaat
     9241 ttaagtggtg tggctaataa tgttgattta tagatgaacc gcctaatcgt taaacctctg
     9301 ttacttcaac atcgatatgt tcaatacggt tgtatgcacc gtgatccaca ggaccaacaa
     9361 aatcattcat tttccaaccg tttttaatag cagaagcgac gaaagctttc gcgctaatca
     9421 cagcttcttt cggtgactta ccgttagcta aatatgcagt tgttgccgca gcaaatgtac
     9481 aaccagcacc atggttataa ctttgttgga acatgtctgt tgttagttga taaaatgttt
     9541 gaccatcata gtataagtca tacgatttat cttgatctaa agctttgcca cctttaatga
     9601 tgacatgctg tgcgccttta tcaaagataa ttgttgcagc ctttttcata tcttcaattg
     9661 aatttaattt acctaatcct gataattgac ccgcttcaaa taagtttggt gtcactaccg
     9721 ttgctttagg tagtaaatat ttaatcatcg cctcagtatt tccaggatta agcacttcat
     9781 cttcgccttt acaaaccatg acaggatcta ctacaaaata ttgtgcatta gatgcctcat
     9841 atacttctcc agcacgtttg attatctcct cagtacctaa catacctgtt ttaatagcat
     9901 caggtccgat tgataaagcc gtttcaagtt gtttttcaaa tacatccatt ggtaatggtg
     9961 taacatcgtg tgaccatgta tctttatcca tagtaacgat ggcagttaaa gcgaccatgc
    10021 catacgtatc taattcttgg aacgttttca aatctgcttg catacctgcg ccagcacttg
    10081 tgtcagaacc ggcaattgtt aaaactttct ttaaagccat tgagcttcac tcctacataa
    10141 taatattgta ttcatcatat catttttaac ctaattgaaa aatattaagc attcaatatt
    10201 tgatgattgt tgaaatgaat cattcatact attgtaactt ttgaaaatgt cattcacttt
    10261 agataagtgt gatatgttaa aatatgtcct gaggtgagat tgaatggaat ggtcgcaaat
    10321 ttttcatgac ataacaacga aacatgactt taaagctatg catgattttt tagaaaaaga
    10381 atattcgact gcaatcgtat accctgatag ggaaaatata tatcaagcgt ttgatttaac
    10441 accgtttgaa aatatcaaag ttgttatatt aggacaagac ccgtatcatg gtccaaacca
    10501 agcacatgga ttagcatttt cagtgcaacc taacgcaaaa ttccctccat ctttacgtaa
    10561 tatgtataaa gaattagcag atgatattgg atgcgttaga caaacaccgc atttacaaga
    10621 ttgggcaaga gaaggcgtct tgttattgaa tacagtttta accgtaagac agggtgaagc
    10681 aaattctcat cgtgatattg gttgggaaac atttactgat gaaattatta aagcagtgtc
    10741 tgattataaa gaacatgttg tctttatttt gtgggggaaa cctgcacagc aaaaaataaa
    10801 gcttatcgat acatctaaac attgtattat aaaatcagtg catcctagtc cactgtctgc
    10861 atatagagga ttctttggat caaaaccgta ttccaaagcg aatgcctatt tagagtcagt
    10921 aggaaaatca ccaattaatt ggtgtgaaag tgaggcgtag atgttgaata gagaaacttt
    10981 aatagcacga attgagcaag aattagtaca agcagagcag gcacagcatg accatgactt
    11041 tgaaaaacat atgtatgcca tacatatatt aacatcttta tatgcttcaa catcaaatac
    11101 accacatatt ggtgaacaac aaatgaatcg tcgtattgct aaccataatc aaatgccaca
    11161 atcacaaata acgcagccaa ctcatcaagt gacagttgct gaaattgaag cgatgggtgg
    11221 taaagtaaat acgcattcag cacatcatca taataagtca tattcacaac cttcaaacca
    11281 acaacaaaga ttagcgacag atgatgacat tggcaatggt gaatccatat ttgattttta
    11341 aaaagcaaca atgaaacata attacttaat agcttgttaa gtatgtaggt taataatcaa
    11401 gacgcatata cttttattcg agtgttcgga tttaaacatt tattaatact gaattatata
    11461 aggagaggta gcaatgaaat tatttattat tttaggtgca ttaaacgcga tgatggctgt
    11521 cggtacaggt gcatttggtg cgcatggttt acaaggaaaa ataagtgatc actatttatc
    11581 agtatgggaa aaagcaacga cgtatcaaat gtaccatggc ttagcattat taattatagg
    11641 tgtaattagt ggtacaactt caatcaatgt taactgggct ggctggttaa tatttgctgg
    11701 tattattttc tttagtggat cattatatat tttagtatta actcaaatta aagttttagg
    11761 tgcgattacg ccaattggtg gcgtattgtt catcattgga tggataatgt taatcattgc
    11821 gacattcaaa tttgctggtt aaattttaaa actttagatt acctatgtaa ctaaacatta
    11881 aatttttaat aaaaataatc aagaaaaaga gttacaaact catcttttgg gtatagaata
    11941 ccttcgaggt gagtttttat ttatggaaaa aaagaataag caaatagata gaggcgattt
    12001 aaaacaaaac ctatctgaaa agtttgtatg ggcgattgca tatggttcat gtatcggatg
    12061 gggcgcattc atcttaccag gagactggat taagcagtca ggtccgattg cagcatcaat
    12121 tggtatagtt attggtgcat tattaatgat attaattgcg gttagttatg gcgcattagt
    12181 agagagattt ccagtatcag ggggcgcgtt tgcctttagt ttcttaagtt tcggcagata
    12241 tgtgagtttc ttctcatcat ggtttttaac ttttggttat gtctgtgtcg ttgctttaaa
    12301 tgcgaccgca ttcagtttac tagttaaatt cttattgcca gatgtcttaa ataatgggaa
    12361 actatacacc attgcgggct gggacgttta tattacggaa atcattattg cgaccgtatt
    12421 actacttgta ttcatgctag taacgattcg tggcgcaagt gtatctggat cattacaata
    12481 ttatttctgt gtggcgatgg taatcgtcgt attattgatg ttctttggtt cattctttgg
    12541 taataatttt gcacttgaaa atttacaacc gttagctgaa cctagcaaag gatggttagt
    12601 gtctattgtg gttattgtat ccgtggcacc atgggcatat gttggatttg ataatattcc
    12661 acaaacagca gaagagttta actttgcacc aaacaagaca tttaagctta tcgtgtacag
    12721 tttattagca gcatcattaa cttatgttgt catgatttta tacactggtt ggttatcaac
    12781 aagtcatcaa agtttaaatg ggcagttgtg gttaacaggt gctgttacac aaacagcatt
    12841 tggttatatt ggattaggtg tattagcaat tgcaattatg atgggtatat ttactggttt
    12901 aaatggattc ttgatgagtt caagtcgctt gttattttct atgggacgtt caggtattat
    12961 gccaacaatg tttagtaaat tacatagtaa atacaaaaca ccatatgtcg caatcatatt
    13021 cctagtagga gtgtcgttaa ttgcaccttg gctaggaaga actgcattga cttggattgt
    13081 agatatgtca tctactggtg tatccattgc ctactttatt acatgtttgt ctgcagcgaa
    13141 attattcagt tataacaaac aaagtaatac gtatgcaccg gtttacaaaa cgtttgctat
    13201 tatcggctca tttgtatcat tcattttctt agcgttgtta ttagtgccag gttctcctgc
    13261 agcactgact gcaccgtctt atattgcatt acttggatgg ttaatcatcg gtttaatatt
    13321 ctttgtgatt cgatatccta aattgaaaaa tatggataat gatgaattaa gtcgcttgat
    13381 tttaaataga agtgaaaatg aagttgatga tatgattgaa gaacctgaaa aagaaaaaac
    13441 taaataataa aagaatcgca caataaacct tcttcattcg gaggcgtatc gtgcgatttt
    13501 ttgtattata aattgacatt taagacgagg cagctgaacc ttatatataa ttgctaagag
    13561 ttagggctga gccatttcta acaaatattt ataatcgttt aaaagatttc acgaacccag
    13621 aaacaattaa tttggaaatt tggtcggcga ataataaacc taatgcgatg gcgcctgcaa
    13681 taagtgtaac ctctagcatg gtattgattg ctgtactgaa atttaataag actaaatttt
    13741 ttgtagcatc gtatgctaag ccaccaggta ctaatggaat gatacccgtt accataaaaa
    13801 tgatggcagg ttctttttgt ttacgagcca tataatgact taacaagcct aatgctaaac
    13861 taccaaagaa actagagtat atagtgtgca cattaaagcc gttgaagaat aaggtgtaaa
    13921 ccatccatcc acacgtacca acgaaaccac atgatagata taattttcta ggtgcatcaa
    13981 aaatgacgca gaagaacatt gaagctaaaa agctaaagat aaagtttaag atccaaaaca
    14041 tagtctgata ctcctatact aaaattaata cgctaccaac gccagcaccg atgccaaacg
    14101 cagtaaccaa tgcttctaat gatttcgttg tgaacatcaa catgtgtcca ccaaataaat
    14161 cttgtattgc gtttgttatt aatacaccag gaacaatagg catgactgcc gcaatgataa
    14221 tagttgccaa gtcacctgtt ggaataagtg tatgtccaat aacggcgata atcccaataa
    14281 ctaatgaacc aatgaattct gggataaact gtgcgtgtaa cttacgatct aaaatctcag
    14341 tgactaggta tcctagacta cctgctaata tcgcagttaa aacatcaatc aatctaccac
    14401 cttgtaaata taagaaactc attgcaatca ttgctgcagc aaaaccttta aagggaagac
    14461 tgctgtcacg cttagcaaca tatatttttt caagttgcgt ttttgcttcg gctaaagaaa
    14521 tttcattgtt tgtaatttga cgcgaaattt tattagcttg agaaattttt attaagtttg
    14581 tatctcgaga ggtaattcta aatattctag gaaacgattc cgaatgtaac gtaaactgga
    14641 tgacagtgtt tgtaacaaag ctgttacttt cactgtaacc aagttttttt gcaatacgtg
    14701 tcatggtatc ttctacacgc gtaccttctg caccagattc taatagtatg cgagcagcaa
    14761 gcatgacaac gtctttgata agtacctctt gtttgtattc ttctgaattt atgtccattt
    14821 catcaccatt gtttataaga atttaatact cattatagtt tatacactat aaaataacca
    14881 catgagcttt ttgataagtt gttattgatt ttaaaaaact aatatttgat actattgtaa
    14941 taataatcat cggaattttt agtagttaaa a
//
LOCUS       DAKTIO010000054        14420 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.054, whole genome shotgun sequence.
ACCESSION   DAKTIO010000054 DAKTIO010000000
VERSION     DAKTIO010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14420
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.054"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(122..2098)
                     /gene="sfaD"
                     /locus_tag="O0293_001256"
                     /gene_synonym="sfnaD"
     CDS             complement(122..2098)
                     /gene="sfaD"
                     /locus_tag="O0293_001256"
                     /gene_synonym="sfnaD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine--citrate ligase SfaD"
                     /protein_id="HCY0280145.1"
                     /translation="MNLNLIFKEQTLKFNKEEQETYLFLQQHNSDWANIFKEMILQGR
                     DKVTQRLVTSMHRENLVKARTQSKKILSRDLIMLDISTTHILEIQFPQAKQTLYAPIT
                     GEHAFDRIDVEGPFYIKDDITNTITRVHHPNEILECILIEAPDLKNAASDQFQQDLIN
                     SATNMTFAISYQALSMQHDSAPLFNIIENSEDSYLRSEQAVIEGHPLHPGAKLRKGLN
                     ALQTFLYSSEFNQPIKLKIVLIHSKLSRTMSLSKDYDTTVHQLFPDLIKQLENEFTPK
                     FNFNDYHIMIVHPWQLDDVLHSDYQAEVDKELIIEAKHTLDYYAGLSFRTLVPKYPAM
                     SPHIKLSTNVHITGEIRTLSEQTTHNGPLMTRILNDILEKDVIFKSYASTIIDEVAGI
                     HFYNEQDEADYQTERSEQLGTLFRKNIYQMIPQEVTPLIPSSLVATYPFNNESPIVTL
                     IKRYQSAASLSDFESSAKSWVETYSKALLGLVIPLVTKYGIALEAHLQNAIATFRKDG
                     LLDTMYIRDFEGLRIDKAQLNEMGYSTSHFHEKSRILTDSKTSVFNKAFYSTVQNHLG
                     ELILTISKASNDSNLERHMWYIVRDVLDNIFDQLVLSTHKSNQVNENRINEIKDTMFA
                     PFIDYKCVTTMRLEDEAHHYTYIKVNNPLYRENN"
     gene            2200..3393
                     /gene="sfaA"
                     /locus_tag="O0293_001257"
     CDS             2200..3393
                     /gene="sfaA"
                     /locus_tag="O0293_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A export MFS transporter"
                     /protein_id="HCY0280146.1"
                     /translation="MTKYFFSSSFLLFLGNWIGQIGLNWFVLTTYHNAVYLGIVNFCR
                     LVPILLLSVWAGAIADKYDKGRLLRITISSSFLVTAILCVLTYSFTAIPISVIIIYAT
                     LRGILSAVETPLRQAILPDLSDKISTTQAVSFHSFIINICRSIGPAIAGVILAVYHAP
                     TTFLAQAICYFIAVLLCLPLHFKVTKIPEDASRYMPLKVIIDYFKLHMEGRQIFITSL
                     LIMATGFSYTTLLPVLTNKVFPGKSEIFGIAMTMCAIGGIIATLVLPKVLKYIGMVNM
                     YYLSSFLFGIALLGVVFHNIVIMFICITLIGLFSQWARTTNRVYFQNNVKDYERGKVL
                     SIIMMDRGMIPLGSLLMSICADVFGIVRTFSIMGISTICITMVFYFINRKLKLKLEES
                     NHGIS"
     gene            3380..5137
                     /gene="sfaB"
                     /locus_tag="O0293_001258"
     CDS             3380..5137
                     /gene="sfaB"
                     /locus_tag="O0293_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A synthetase SfaB"
                     /protein_id="HCY0280147.1"
                     /translation="MVYLEWAKADRNIQYRVINAIIKERIYPEQTFISQKGSLIEIQY
                     HMHVLTIEVVRKSALERYEFTGDITYLNKGETSLIITLEGLLDVLNHDFDIPISERLR
                     EELIHSRDSLVETYKQMSHRQTLISQSFKFSRLPQDINFFSWLQHVKDSDKTDDLTYS
                     ESLVPEGHPTHPLTKTKLPLTMEEVRAYAPEFEKEIPLQIMMIEKDHVVCTAMDGNDQ
                     FIIDEIIPEYYNQIRVFLKSLGLKSEDYRAILVHPWQYDHTIGKYFEAWIAKKILIPT
                     PFTILSKATLSFRTMSLIDKPYHVKLPVDAQATSAVRTVSTVTTVDGPKLSYALQNML
                     NQYPGFKVAMEPFGEYANVDKDRARQLACIIRQKPEIDGKGATVVSASLVNKNPIDQK
                     VIVDSYLEWLNQGITKESITTFIERYAQALIPPLIAFIQNYGIALEAHMQNTVVNLGP
                     HFDIQFLVRDLGGSRIDLETLQHRVSDIKITNDSLIADSIDAVIAKFQHAVIQNQMAE
                     LIHHFNQYDCVEETELFNIVQQVVAHAINPTLPHANELKDILFGPTITVKALLNMRME
                     NKVKQYLNIELDNPIKKEV"
     gene            5144..6214
                     /gene="sfaC"
                     /locus_tag="O0293_001259"
     CDS             5144..6214
                     /gene="sfaC"
                     /locus_tag="O0293_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A biosynthesis protein SfaC"
                     /protein_id="HCY0280148.1"
                     /translation="MAHVNINISKIKYNAKVLQTVFQSKNMQFTPVIKCIAGDRTIVE
                     SLKALGINHVAESRLDNIISIADQDLTYTLLRTPAKKEISDMIEKVDMSIQTELSTIH
                     QINEVAEVLGKKHKILLMVDWKDGREGVLTYDVLDYIKEIIHLKNIHFVGLAFNFMCF
                     KSDAPSDDDIFMINRFVSAVEREIGYRLKIISGGNSSMLPQLLYNDLGKINELRIGET
                     LFRGVDTTTNQAIAMLYQDAITLEAEILEIKPRVNTQTHESFLQAIVDIGYLDTKVDN
                     ISPMDQHINILGASSDHLMLDLNGQGHYQVGDHISFSLNYEALSHSMYMKNLHKVYID
                     DSKIDTLLQNFDVKSPAMVNHY"
     gene            complement(6315..6440)
                     /locus_tag="O0293_001260"
     CDS             complement(6315..6440)
                     /locus_tag="O0293_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280149.1"
                     /translation="MYHCYIIFALIQLTPFSMTVIPVIQLILSVAILCDELLHLI"
     gene            6606..7589
                     /locus_tag="O0293_001261"
     CDS             6606..7589
                     /locus_tag="O0293_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0280150.1"
                     /translation="MRGLKTFSILGLIVALLLVAACGNTDNSSKKESSTKDTISVKDE
                     NGTVKVPKDAKRIVVLEYSFADALAALDVKPVGIADDGKKKRIIKPVREKIGDYTSVG
                     TRKQPNLEEISKLKPDLIIADSSRHKGINKELNKIAPTLSLKSFDGDYKQNINSFKTI
                     AKALNKEKEGEKRLAEHDKLINKYKDEIKFDRNQKVLPAVVAKAGLLAHPNYSYVGQF
                     LNELGFKNALSDDVTKGLSKYLKGPYLQLDTEHLADLNPERMIIMTDHAKKDSAEFKK
                     LQEDATWKKLNAVKNNRVDIVDRDVWARSRGLISSEEMAKELVELSKKEQK"
     gene            7601..8632
                     /locus_tag="O0293_001262"
     CDS             7601..8632
                     /locus_tag="O0293_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCY0280151.1"
                     /translation="MAIKEISSQSAIDHKRKRRTTLTYIVSLCFLFICIYLNMAIGSS
                     KINFSDIIHYVTGHTDTKATFLLHNVRMPRMIAGLFIGGALAVSGLLMQAMTRNPLAS
                     PKIFGVSSGASFFIVFVTIIIPALEYYALYLGVIGAFIGGLTVYTLSGATKGMTPIKL
                     ALAGMAIHLFFSSMTEGIIILNENSNEQVMFWLVGSLSSMKWDEILTILPWIIGALIV
                     TIFIGRQLTIMELGDDIAKGLGQNINKVRIIIGLLVIILTGMSVSVAGPIGFVGLIVP
                     HIVKRYVSKNYLVMIPLTFIIGADLLLLSDVLSRLITYPYESPVGIVTSFVGALYFLF
                     ITIKGVKRI"
     gene            8629..9597
                     /locus_tag="O0293_001263"
     CDS             8629..9597
                     /locus_tag="O0293_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCY0280152.1"
                     /translation="MMTTKIIRRYSLITILLIVSIFISLCVGSVMIHPIDAIKGIFTQ
                     DDFILNEYRIPRTLLGIIIGSSLAISGAIIQAVIRNPLASPDVIGISKGASLAAVIII
                     MTFPTAPLFVLPIGSFLGAFAVSLFLSFLISKYDVKGSKLALIGLAIGAICTAIVQFL
                     LIRNPLEANKALVWLTGSLYGHDMSNIYTILPWFLITIPIIILLSYQLDVLNLGDAVA
                     TALGLKVKTIKMLLLILAVILAGSAISVVGGISFLGLIAPHIARQLIGNKTLHVIIMS
                     GLIGAILLTFGDGLARGIHPPLDIPVGVIIAIIGAPYFLILLRRMK"
     gene            9833..11203
                     /locus_tag="O0293_001264"
     CDS             9833..11203
                     /locus_tag="O0293_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCY0280153.1"
                     /translation="MDSFSRKEIVIGRLKFITMSLIGILLFLVPIPVEQDGQKQTTLP
                     VAFLAGVLKDVLGGVMPFLIVTIITLSGIITLICSTILKDKLKPDGLMNNAFNVRIGW
                     LILRILAVVFAWMTFLRIGSKVIYSDETGGLLFSSLLPTLVAVFLFAALFLPLLMEYG
                     LLEMLGPIFRPVMRPLFTLPGRSTVDNLASFIGDGTVGVLITSRQYGEGYYSRREATV
                     ISTTFSVVSITFAIVVAETVHMQNQFFAFYLSVIVSCLVAAVIMPRIWPLNKIPDEYA
                     KEVPESARTEALPEGKTALRHGFDTATEVGIKAPGVIDFFKSGLKTVIDMWFVILPVV
                     MSIGTIATIIANYTPFFVILGKPFVPFLELMQIPEAAQASQTIIIGFADMFLPSILIE
                     GVQNDITRFVIGALSISQLIYLSEVGGVILGSKIPVSIGKLFMIFLIRTIITLPIISL
                     MAHLLL"
     gene            complement(11335..11595)
                     /locus_tag="O0293_001265"
     CDS             complement(11335..11595)
                     /locus_tag="O0293_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280154.1"
                     /translation="MAMTVKKDNNEVRIQWRVADIKIPTSEIKNITQDQDIHAVPKLD
                     SKDVSRIGSTFGKTNRVIIDTEDHEYIIYTQNDQKVYNELTK"
     gene            11772..12287
                     /locus_tag="O0293_001266"
     CDS             11772..12287
                     /locus_tag="O0293_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCY0280155.1"
                     /translation="MTGKTHASCGMLVGALTTQYFQTDIFSSVTVIILATLASLLPDI
                     CHTQSKIGRRFKVISFFVRLIFGHRTFTHSILFIAIIAFLLQIIQTPNYYMAAIIIGL
                     VSHVILDMITPKGVKLFYPLPFNVKTPIQFKTGGLVDLSLATALMVGTVYVLFQPFIN
                     DIISNWNTKFL"
     gene            12331..13518
                     /locus_tag="O0293_001267"
     CDS             12331..13518
                     /locus_tag="O0293_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HCY0280156.1"
                     /translation="MLDKNQLAKYKQDHLCEYEKIMSNNEKEALEEKVASLDLDFIAK
                     LYNDLYINKKTIDDVSAVSEVKYDIKSQMSDDEIKRLEEQGLQAIKEGQFAVLLMAGG
                     QGTRLGYKGPKGSFEIEGVSLFELQANQLKTLNHQSGHTIQWYIMTSDINHEETLAYF
                     EAHSYFGYDQEAIHFFKQDNIVALSEEGKLILNQQGRIMETPNGNGGVFKSLDKAGYL
                     EEMSNNGVKYIFLNNIDNVLVRVLDPLFAGFTVEHDYDITSKTIQPKPGESVGRLVNV
                     DCKDTVLEYSELDPEVANQFNNANIGIHAFKLGFILNAVNRELPYHLAIKNLKQLDEN
                     FGVIEQPTLKFELFYFDIFTYGTSFVTLQVPREEEFSPLKNKEGKDSVATATEDLRRM
                     GLI"
     gene            13537..14220
                     /locus_tag="O0293_001268"
     CDS             13537..14220
                     /locus_tag="O0293_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCY0280157.1"
                     /translation="MSKSTKKSNDTIVETFKDIIPLSFGEEIGNAASHGVAALLTLLV
                     LPYAAVHSYIAHGTLASVSMSIYVISIFMMFISSTIYHSMQNETPHKYILRIIDHSMI
                     YVAISGTYTPILLTVVGGWIGWTVFILLWGTTLWGILYKSIAVKVNQKLSLIVYLVMG
                     WVGIILLPIIFIRTSWIFILFIFLGGVSYTIGAWFYAQKNRPYFHMIWHIFIVIASLL
                     HLIAILYFM"
BASE COUNT         4794 a         1999 c         2627 g         5000 t
ORIGIN      
        1 cctgtttttt gtgattttta ataatttgaa tatggaaaat gtattattct ctcatttgta
       61 tagattgtat ttaataagtt aatgtaatcc ttgagctcac gattaataaa attctataac
      121 cttaattatt ttctcgatac aaagggttat taactttaat ataagtataa tgatgtgcct
      181 catcttcaag acgcattgtt gtaacacact tataatctat aaatggcgcg aacatggtat
      241 ctttaatttc atttatgcga ttctcattta cttgattaga tttgtgtgtc gaaagtacaa
      301 gttgatcaaa aatgttatct agtacatcac gaacgatata ccacatatgt ctttctaagt
      361 ttgaatcatt tgatgcttta gagattgtaa gaattaattc gcctaaatgg ttttgaacgg
      421 tagaataaaa ggctttgtta aacacagacg tttttgaatc agtaagaatt cttgattttt
      481 catggaaatg agatgtactg tatcccattt catttagttg tgctttatca attcttaaac
      541 cttcaaagtc tctaatatac atcgtgtcca atagaccatc tttccgaaat gttgcaatag
      601 cattttgcaa gtgagcctct aatgcaatgc catatttagt aactagtggg attacgagac
      661 caagcaatgc tttactataa gtttcaaccc acgatttcgc cgatgattca aaatcagaca
      721 atgatgcagc tgattgataa cgtttaatca atgtcacgat aggtgattca ttgttaaatg
      781 ggtaggttgc aactaagctt gaaggaatca atggtgtgac ttcttgtgga atcatttggt
      841 atatattttt tctaaataat gtacctaatt gttcacttcg ttcagtttga tagtctgctt
      901 cgtcttgctc attataaaaa tgaataccag caacttcatc aataattgtt gatgcatagg
      961 acttaaatat gacatctttc tccaaaatat catttaaaat acgtgtcatt agtggaccat
     1021 tgtgcgtcgt ttgttctgat aatgtacgaa tctcacctgt aatatgaacg tttgtcgaca
     1081 atttgatgtg tggcgacata gctgggtatt taggaactaa tgttctgaaa gataaaccag
     1141 cataataatc caacgtatgt tttgcttcaa tgattaattc tttatctact tctgcttgat
     1201 aatcagaatg taatacgtca tctaattgcc atggatgaac aatcataatg tgataatcat
     1261 taaagttaaa ctttggcgta aattcatttt ctaattgttt aattaagtcc ggaaatagtt
     1321 gatgaacagt tgtatcataa tctttagaca gtgacatagt acggcttaat ttactgtgaa
     1381 tcagtactat tttcaactta ataggttgat tgaattctga agaatatagg aatgtttgta
     1441 atgcatttaa acctttacgt aatttagccc caggatgtag cggatgacct tcaataacgg
     1501 cttgctctga acgcaagtaa ctatcttcgc tattttcgat aatattaaat aaaggtgcag
     1561 aatcatgttg cattgacagt gcttgatagc taattgcaaa tgtcatatta gttgcactgt
     1621 ttattaaatc ttgctgaaat tgatcactag cagcattttt taaatctggt gcttcaatta
     1681 aaatacactc aagaatttca tttggatggt gtactcgtgt aatcgtattt gtaatgtcat
     1741 ctttaatgta gaaagggcct tcaacatcaa ttcgatcaaa ggcgtgttct ccagtgatag
     1801 gagcatataa tgtttgctta gcttgtggga actggatttc tagtatatga gtcgttgaga
     1861 tatctaacat aatcaaatca cgactcaata ttttcttact ttgagtgcgc gctttaacta
     1921 agttttcgcg atgcattgat gtgaccaatc tctgagtgac tttatctctt ccttgtaaaa
     1981 tcatctcttt aaaaatatta gcccaatcgc tattatgttg ttgtaaaaat aaatatgttt
     2041 cttgttcttc tttgttaaat tttaatgtct gttctttaaa aattaagttc aagttcataa
     2101 ttcacctcta tgaaatattt tacaaaagca agatagattt gtataatcca tattaatgat
     2161 aatgattctt attatcaaca gaatgcgggt gtaagtttta tgacaaaata tttttttagc
     2221 agttcttttc tactatttct aggtaattgg attggacaaa tagggctaaa ttggtttgta
     2281 cttaccactt atcataacgc agtttatctg gggattgtca atttttgcag acttgtacca
     2341 atattattac taagtgtgtg ggcaggggca attgccgata aatatgataa agggcgattg
     2401 ctgagaatta caatttcatc atcattttta gtaactgcaa ttttatgtgt gctcacgtat
     2461 agtttcactg caattccaat tagcgtcatt attatatatg cgacattaag agggatttta
     2521 agtgcggttg aaacaccttt aagacaagca atcttaccag atttatcaga taaaatatct
     2581 actacacaag ctgtatcatt tcattcattc atcattaata tttgtcgttc aatagggcct
     2641 gccattgctg gtgtcatatt agcagtctat catgcgccaa caacatttct tgcacaggca
     2701 atttgttatt ttatcgcagt tttattatgc ttaccattac attttaaagt aactaaaata
     2761 cctgaagatg catcaagata catgccgtta aaagttatta tagattactt caaattacat
     2821 atggaaggtc gacaaatatt tataacatca ttattgatta tggcgacagg tttttcatat
     2881 acgacacttt taccagtttt gacaaacaaa gtatttccgg ggaaatctga aatatttggt
     2941 atcgctatga cgatgtgtgc cattggtggt attattgcaa cgctagtttt acctaaagta
     3001 cttaaatata ttggtatggt aaatatgtat tatttaagtt catttttatt tggcattgct
     3061 ttgttaggtg tggtatttca caatattgtc atcatgttca tttgtattac attgattggg
     3121 ttatttagtc aatgggcacg tacgacaaat cgcgtttatt ttcaaaataa tgttaaagat
     3181 tatgaacgtg gtaaagtact gagtattatt atgatggata gaggtatgat tccattggga
     3241 agtctattaa tgagtatatg tgcagatgtg tttggcattg ttagaacttt ttcaataatg
     3301 ggaataagta ctatatgcat tacaatggta ttctatttta taaatagaaa gttgaagtta
     3361 aagttggagg aaagtaatca tggtatatct tgaatgggca aaggcagata gaaatattca
     3421 atatcgtgta attaacgcca ttattaaaga acgtatttac cccgagcaaa catttatttc
     3481 gcaaaaagga tctttaattg aaattcagta tcatatgcat gtgttgacta ttgaagttgt
     3541 tagaaaaagt gcattagaac gctatgagtt tacaggtgat attacttatt taaataaagg
     3601 tgaaacgtca ttaattataa ctttagaagg tttattagat gtgttgaatc atgactttga
     3661 tatccctatt tcagagcgac tacgcgaaga gttaatacac agtcgagata gtttagttga
     3721 aacatataag caaatgtctc acagacaaac gttaataagt caaagtttta aattttcaag
     3781 gttaccacaa gatattaact ttttttcatg gttacaacat gtaaaagata gtgataagac
     3841 agatgattta acttattctg agagtttggt accagagggg catccaacac accctttaac
     3901 caaaacgaaa ttgcccttaa ctatggaaga agtacgagca tatgcacctg agtttgaaaa
     3961 agaaatccct ttgcaaatta tgatgattga aaaagaccat gttgtgtgca cagctatgga
     4021 tggtaatgat caatttatta ttgatgaaat aattcccgaa tactacaatc agattcgtgt
     4081 gtttttaaag agtttaggtt tgaaaagtga agactataga gcgattttag tacatccttg
     4141 gcaatatgat catacgatag ggaaatattt tgaagcatgg attgctaaaa aaatattaat
     4201 tccaacgccg tttacaatac tttcaaaagc aactttatca tttaggacga tgtctttaat
     4261 tgataaacca taccatgtta agttgcccgt cgatgcacaa gcaacaagtg ccgttagaac
     4321 agtctcaact gtgactactg tagatggacc aaagttaagt tatgctttac aaaacatgtt
     4381 gaatcaatat ccaggattta aagttgctat ggaaccgttc ggtgaatatg caaatgttga
     4441 taaagatagg gcacgtcagc ttgcttgtat tataagacaa aagcctgaaa ttgatggaaa
     4501 aggtgcaaca gtagttagtg caagtctagt taataaaaat ccaatagatc aaaaagttat
     4561 cgtggatagt tacttagagt ggttaaatca aggaattact aaagaaagta ttacgacatt
     4621 tattgaacga tacgctcaag cattaatccc gcctttaatt gcttttattc aaaattatgg
     4681 aattgcttta gaagcacaca tgcaaaatac agtagtgaac ttggggccac attttgacat
     4741 tcaattttta gtgagagatt taggtggttc tagaattgat ttagaaacat tacaacatcg
     4801 tgtatcagat attaaaatta caaatgatag tttaatagct gattctatag atgcagtgat
     4861 tgcaaaattc caacatgctg ttattcaaaa tcaaatggca gaattaatcc atcattttaa
     4921 tcagtatgat tgtgttgaag aaaccgaatt atttaacata gtacagcaag tagtagcgca
     4981 tgccattaac ccaacactac cacatgcaaa tgagttaaaa gatattttgt ttggaccaac
     5041 aattactgtc aaagcgttgt taaatatgag aatggaaaat aaagtaaagc aatatttaaa
     5101 tattgagtta gataatccga taaaaaaaga ggtgtagtac tacatggcac acgttaacat
     5161 aaatatatcg aagattaaat ataacgccaa agtacttcaa acagtttttc aaagtaaaaa
     5221 tatgcaattc acaccagtaa ttaagtgcat agctggtgac cgtacaattg tagaaagctt
     5281 aaaagcgtta ggtatcaatc atgttgcaga atccagattg gataacataa ttagtattgc
     5341 agatcaggat ttaacatata cgttattgcg tacgcctgcc aaaaaagaga tttcagatat
     5401 gatagaaaaa gttgatatga gtatccaaac agaattatct accatacatc aaataaatga
     5461 agtagcagaa gttttaggaa aaaaacataa gattttatta atggtagatt ggaaagatgg
     5521 ccgtgaaggt gttctgactt atgatgtttt agattatatt aaagagatta ttcacttgaa
     5581 aaatatccat tttgtaggtt tagcattcaa ttttatgtgt tttaaatctg atgcgccaag
     5641 tgatgatgat atctttatga ttaatcgatt cgtctcagct gttgaaagag aaataggata
     5701 tcgattgaaa attatttcag gtggtaattc gagcatgtta ccacaacttt tatataatga
     5761 tttgggtaaa ataaatgaat tgcgaatagg tgaaacgttg tttagaggtg ttgatacaac
     5821 gacaaatcaa gcgatcgcta tgttatatca agatgcgatt acattggagg ctgagatttt
     5881 agaaattaaa cctcgagtta atacacaaac gcatgaatca ttcttacaag ctattgtaga
     5941 tattggttat ttagatacaa aagtcgataa tatttcacct atggatcagc atatcaatat
     6001 tttaggcgct tcgagtgatc atttaatgct tgatttaaat ggtcaaggac actatcaagt
     6061 tggagatcat atatcttttt cattaaatta tgaagcactt tctcattcga tgtatatgaa
     6121 aaatttacat aaggtatata ttgatgattc aaagattgat acgctacttc aaaattttga
     6181 tgtgaaatct cctgctatgg taaaccacta ttaatatatt tatcaataag tctaagttga
     6241 caagttatac tacatatcgt ttgcacggtt gtatcataat tgttcaactt agattttttg
     6301 tatttgttga tttatcaaat taagtgcaac agttcgtcac ataaaattgc aacagataat
     6361 atcagctgaa ttacagggat aacggtcatg ctaaatggtg tcaattgtat taatgcaaag
     6421 atgatatagc aatgatacat tatcagtatt ttgtctaagg aaatgtgcta attgtagtca
     6481 taattattag aggaaatata tagtcataca ttttagaaat acaaaaaaga ttgaacgcta
     6541 cttgacaatg ataattgtta tcaataaaat aataaatgaa gttatacata ttaaggagtg
     6601 gaacgatgag aggtctaaaa acttttagta tattgggatt aatagttgcc ttacttttag
     6661 ttgcagcttg tggtaatacg gataattcaa gtaaaaaaga atcatcaact aaagatacta
     6721 tttcggtaaa agatgaaaat ggtacagtaa aagtacctaa agatgcaaaa cgtatcgttg
     6781 tattagagta ctcatttgca gatgcattag cagcattaga cgttaaacca gttggtattg
     6841 ctgatgatgg taagaaaaaa cgtatcatta aaccagttag agaaaaaatt ggggattata
     6901 cttctgtagg tacacgtaaa cagccaaact tagaagaaat tagtaaatta aaaccggatt
     6961 taattatcgc tgatagcagt agacataaag gtattaataa agaattaaac aaaattgcac
     7021 caacattatc attaaagagt tttgatggag actacaaaca aaatattaat tcgttcaaaa
     7081 caattgctaa agctttaaat aaagaaaaag aaggcgaaaa acgtcttgct gagcatgata
     7141 aattaatcaa taagtataaa gatgaaatta aatttgatag aaatcaaaaa gtgcttccag
     7201 cagtagttgc taaagctggt ttattagcac atccaaacta ttcatatgtt ggacaatttt
     7261 taaacgaact aggatttaaa aatgcattaa gtgacgatgt aacaaaaggt ttaagtaaat
     7321 atttgaaagg accttactta caattagaca ctgaacattt agctgattta aatccagagc
     7381 gtatgatcat tatgacagat catgctaaaa aagattctgc tgaattcaag aagttacaag
     7441 aagatgcaac atggaaaaag ttgaatgcag ttaaaaataa tcgcgtggat attgttgacc
     7501 gtgatgtttg ggcaagatct cgtggcttaa tttcttctga agaaatggct aaagaacttg
     7561 ttgaattatc aaaaaaagaa caaaagtaag gtggaagtaa atggctataa aagaaataag
     7621 tagccaatct gccatagatc ataaaagaaa aagacgcaca acactcacgt atatagtgag
     7681 tttgtgcttt ctttttattt gtatatattt aaatatggcg attggttctt cgaaaattaa
     7741 ttttagcgat atcattcact atgttactgg tcatacagat acgaaagcaa cgtttttatt
     7801 gcataatgta cgtatgccaa ggatgattgc agggttattt attggcggtg cattagcggt
     7861 atctggtttg ttaatgcaag caatgacacg caatccgcta gcatcaccta aaatttttgg
     7921 tgtcagttct ggtgcatcgt ttttcattgt atttgtgacg attatcattc cagcattaga
     7981 atattatgca ttatatttag gtgtgatagg tgcatttata ggtggtttaa ctgtttatac
     8041 actttcaggt gctacgaaag gtatgacacc cattaaatta gcattggctg gtatggccat
     8101 ccatttgttt tttagtagta tgaccgaagg tattattatt ttgaatgaga attcaaacga
     8161 acaagtgatg ttttggcttg tcggttcatt atccagtatg aagtgggatg aaattttaac
     8221 cattctacca tggattattg gtgctttaat tgtaacgatt tttattggac gtcaactaac
     8281 gataatggaa ctaggcgatg acattgctaa gggattgggt caaaatataa ataaagttag
     8341 aattatcatt ggactattag tcattattct tacaggtatg tcggtgtcag tggcaggacc
     8401 aattggattt gttggcttaa tcgtaccgca tatagtcaaa cgctatgtga gtaagaatta
     8461 tttagtcatg attccattaa catttattat aggtgcagat ttgttactac tatcagatgt
     8521 attaagtcgt ttaattacat atccttatga atcaccagta gggattgtta catcattcgt
     8581 aggtgcactt tacttcttat ttataacgat taaaggggtg aaaagaatat gatgacaacg
     8641 aaaattatac gtcgttatag tttaatcaca atccttttaa ttgtgagtat attcataagt
     8701 ttatgtgtag ggtctgtcat gattcatccg attgatgcta ttaagggaat ctttacacaa
     8761 gatgatttta ttttaaatga gtatcgtata cctagaacat tacttggtat tattatcggt
     8821 agtagcttgg ctatttcagg tgcaattata caagctgtaa taagaaaccc gttagcctca
     8881 cctgatgtta taggtatttc taaaggtgcc agtttagctg ctgtgattat tattatgacc
     8941 tttccaacag caccattgtt tgtattacct ataggttcat tcttaggtgc ctttgcagta
     9001 agtttatttt tatcattttt aatttcaaaa tatgatgtta aaggttcaaa gcttgcctta
     9061 attggtttag caataggcgc aatatgtacg gcgattgtgc agttcttatt aatcagaaat
     9121 ccactggaag caaacaaagc gcttgtttgg ttgacaggta gtctatatgg acatgatatg
     9181 tcgaatattt atactatatt accttggttt ttaataacga taccaatcat cattttattg
     9241 agttatcaat tagatgtatt gaatctaggt gatgctgttg caaccgcact cggtttaaaa
     9301 gttaaaacta ttaaaatgct attgttaatt ttagcagtaa tattagcagg atccgcaatt
     9361 tcagttgtcg gtggtattag ctttttagga ttaattgcac ctcatattgc gagacaatta
     9421 ataggaaata aaacattaca cgttattatc atgtcggggt taattggtgc tatattgtta
     9481 acatttggag atggtttagc ccgtggcatt catccgccac tggatattcc tgttggggtt
     9541 atcattgcaa ttatcggtgc accatacttc ttaatattat tacgaagaat gaaataacta
     9601 acatatgatt agagtttaaa aaaggttaag tacgaaatca gtaattgtac ttaacctttt
     9661 ttgaattcat cttgaaaaat tacaagaaat agcgtatatt taataactgt aattattcat
     9721 tattttgcat acataaacat tttgaaagat agaatgataa ggaatatgtt tgttaactga
     9781 atagaattct gtatatactt aaaacgaata catagagaaa tggattgatt ttatggatag
     9841 cttttcaagg aaagagatag ttattgggag acttaagttt attacgatgt ctttaattgg
     9901 tatcttatta tttttagtac caattccagt cgaacaagat ggacagaagc aaacaacgtt
     9961 gcccgtggca tttttagcag gcgtgcttaa agatgtatta ggtggcgtaa tgccattttt
    10021 aattgtgacg ataattactt tatcgggtat cataactctg atttgttcaa ctattttaaa
    10081 agataaattg aaacctgacg ggctaatgaa caatgctttt aacgttagaa ttggttggtt
    10141 aattttacga atattagcag ttgtttttgc atggatgaca tttttacgaa ttggttcaaa
    10201 ggttatttac tcagatgaaa cgggtggatt attgttctca agtttattgc caacattagt
    10261 ggcagtattc ttatttgcag ctctattttt accgctatta atggaatacg gtttgttaga
    10321 gatgttgggt cctattttta gaccggttat gagaccttta tttacattgc caggacgttc
    10381 gacggtagat aatttagcat catttattgg agacggaact gttggtgtct taattacaag
    10441 tagacaatat ggtgaaggat attattctcg tcgtgaggca acagttattt caacgacatt
    10501 tagtgtagtt tctatcacct ttgcaattgt agttgctgaa acagtgcaca tgcaaaatca
    10561 attttttgct ttctatttat cagtgatagt atcctgcctc gttgcagcag tcattatgcc
    10621 aagaatttgg cctttaaata aaattcctga tgagtatgct aaggaggtac ctgaaagtgc
    10681 gcgtactgag gcattgccag aaggaaagac ggcattgaga catggctttg atactgcaac
    10741 agaagtaggt attaaagcac cgggcgttat tgactttttt aagtcaggat taaaaacagt
    10801 cattgatatg tggtttgtta ttttgcctgt agtaatgagt attggtacta ttgctaccat
    10861 tattgcgaac tacacgccct tttttgtcat attgggtaaa ccttttgttc catttttaga
    10921 gttaatgcaa atacctgaag cggcacaggc ttctcaaacg attattatcg gttttgccga
    10981 tatgttttta ccttctatat taattgaagg tgtacaaaat gatataactc gttttgttat
    11041 tggggcactt agtatatcgc agttaatata cttgtctgaa gtaggtggcg tcatacttgg
    11101 ttcgaagatt cctgtaagca tcggcaaatt gtttatgatt ttcttgatta gaacaatcat
    11161 tacactacca attatttctt tgatggcaca tcttttgtta taaatatatg tgaaaaagga
    11221 caactgttaa aattatggga acagttgtcc ttttgtattt gtttatgctg ggtgttgaat
    11281 caattaaaaa aatcattcat gaagccctca ccatgaatga tttttttata caatttattt
    11341 agttaattca ttgtaaactt tttgatcatt ttgagtataa ataatgtatt cgtggtcttc
    11401 agtatcgata ataacgcgat tcgttttacc aaacgttgag ccgattctag atacatcttt
    11461 gctgtctaat ttaggaactg catgaatatc ttggtcttgt gtaatatttt taatttcact
    11521 tgtaggaatt ttgatatcag caactctcca ttgaatacgc acttcattat tatccttttt
    11581 tactgtcatt gccattattc aaacacatcc tttaaatatt ttgtaaatac atcataaacg
    11641 attttgtaat cgttttcaag agttatgtga tactttttta aattgtgtca tactaatgaa
    11701 ttttaacccg aaaatatgtt cgtgtttttg ttagggatgt ggtaaaatgt gagtattaaa
    11761 ggagaacaaa tatgacaggt aaaacacatg cttcatgcgg catgctcgtt ggagcgctaa
    11821 caacacaata ttttcaaacg gatatttttt cgtcggttac ggttatcatt ctagcaactc
    11881 tagctagctt attacccgat atatgtcaca ctcaaagtaa gattggcaga aggtttaagg
    11941 taattagttt ttttgtgaga ttgatctttg gacatcgaac ctttacgcat tcaatattat
    12001 ttatcgcaat catagcattt ttattgcaaa ttattcagac gcctaattat tatatggcag
    12061 caattataat tggattagtg tcacatgtta tactagatat gataacacca aaaggcgtga
    12121 aattatttta tccgttaccg tttaatgtga agacaccgat tcaatttaaa acaggtggac
    12181 ttgtagactt atctttagct acagcgttaa tggtcggaac agtttatgtt ttatttcagc
    12241 ctttcatcaa tgatataata agcaactgga atactaagtt tttatagaaa ataaattatt
    12301 aaaggttaca aactcagcag gaggcataaa atgctagata aaaatcaatt agctaaatat
    12361 aaacaagatc atttgtgtga atatgaaaaa ataatgagta acaatgaaaa agaagcactt
    12421 gaagagaagg tagcgtcatt agatttagat tttattgcca aattatataa cgatttatac
    12481 atcaataaga aaacaataga tgatgtatct gcagtttctg aagtgaaata tgatattaaa
    12541 tcacaaatga gtgatgatga gattaagcgt ttagaggaac aaggacttca agctattaaa
    12601 gaaggacaat ttgcagtact tttgatggca ggtggtcaag gaacaagact tggttacaag
    12661 ggccctaaag gttcgtttga aattgaaggt gtaagtttat ttgaacttca agctaaccaa
    12721 ttaaaaacat tgaatcatca atctggacat acgattcaat ggtatattat gacaagtgat
    12781 atcaatcatg aagaaacttt agcttatttc gaagcacata gttattttgg atatgatcaa
    12841 gaagcaatcc atttctttaa acaagataat attgtggcgc ttagtgaaga agggaagtta
    12901 attttaaatc aacagggtcg tattatggaa acgccgaatg gtaatggtgg cgtgtttaaa
    12961 tctttagaca aggcaggata cttagaagaa atgtctaata atggcgttaa gtatattttc
    13021 ttgaataata tcgacaatgt tttagtaaga gtgttggatc cactatttgc aggatttacg
    13081 gtagaacatg attacgatat tacttctaaa acgattcaac caaaaccagg agaaagtgtt
    13141 ggacgtttag ttaatgtcga ttgtaaagat acagtactgg agtattcaga attagatcca
    13201 gaagttgcaa atcaatttaa caatgctaat atcggtattc atgcatttaa acttggtttt
    13261 attttaaacg ctgtaaatcg tgagttacct tatcatttag ctattaagaa tttgaagcaa
    13321 ttagatgaga actttggtgt tattgaacaa ccaactttaa aattcgaatt gttctatttt
    13381 gacatattta cttatggaac tagttttgtc acgttacaag ttcctagaga agaagaattt
    13441 tcacctctta aaaataaaga aggtaaagat agtgttgcaa ctgcaacaga agatttacgt
    13501 agaatgggtt taatttaatg ataggcggtg actatgatgt ccaaatcaac taaaaaaagt
    13561 aatgacacaa tcgtagaaac atttaaggat atcatccctt tatcatttgg agaagaaatt
    13621 ggtaatgcgg catctcatgg tgttgcagct ttgttaacat tattagtgtt accctatgcg
    13681 gctgtgcaca gttatattgc acatggtacg cttgcgtctg tgagtatgtc tatttatgtt
    13741 atttctatct ttatgatgtt tatttcatct actatttatc attctatgca aaatgaaaca
    13801 cctcataaat atattttaag gattatcgat cacagtatga tttacgtagc aatttctggt
    13861 acatatacgc ctattttgtt aacagttgta ggtggttgga taggttggac agtatttatt
    13921 ttattatggg gaacgacact ttgggggatt ttatataaat ccatagctgt caaagttaac
    13981 caaaagttaa gcttaattgt ctatttagtg atgggctggg taggtattat cttgttacca
    14041 attattttta ttcgcacatc atggatattc atattattca ttttcttagg tggtgtgtct
    14101 tatacaattg gtgcatggtt ttatgcgcaa aagaatagac catattttca tatgatttgg
    14161 catattttta ttgtaatagc atcactattg catttaattg caatcttata ttttatgtag
    14221 tcgtttagaa aagctggggg aaactccgct ttttttatta cataaatcat tctaataggg
    14281 gattgtattg taatagaatt atcgttaaga tttgaaagtc aaaagtgttt atacatatga
    14341 aaaccaatta tttgtaaata aaatcttata aataaatata tagaaggagg tatttcatgc
    14401 gattaaagtc aatcataact
//
LOCUS       DAKTIO010000055        14158 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.055, whole genome shotgun sequence.
ACCESSION   DAKTIO010000055 DAKTIO010000000
VERSION     DAKTIO010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14158
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.055"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            41..274
                     /locus_tag="O0293_001269"
     CDS             41..274
                     /locus_tag="O0293_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HCY0280158.1"
                     /translation="MDYIGQYAVIHLVLHVVCICIAYWALQSIRLDQFFKKGYATQLQ
                     VCMIFVAILLGTAVSNFIVDLLQYSTQVKYLIK"
     gene            385..1650
                     /gene="murA"
                     /locus_tag="O0293_001270"
     CDS             385..1650
                     /gene="murA"
                     /locus_tag="O0293_001270"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCY0280159.1"
                     /translation="MDKIVIKGGNKLTGEVKVEGAKNAVLPILTASLLASDKPSKLVN
                     VPALSDVETINNVLTTLNADVTYKKDENAVVVDATKTLNEEAPYEYVSKMRASILVMG
                     PLLARLGHAIVALPGGCAIGSRPIEQHIKGFEALGAEIHLENGNIYANAKDGLKGTSI
                     HLDFPSVGATQNIIMAASLAKGKTLIENAAKEPEIVDLANYINEMGGRITGAGTDTIT
                     INGVESLHGVEHAIIPDRIEAGTLLIAGAITRGDIFVRDAIKEHMASLVYKLEEMGVE
                     LDYQEDGIRVRAEGELQPVDIKTLPHPGFPTDMQSQMMALLLTANGHKVVTETVFENR
                     FMHVAEFKRMNANINVEGRSAKLEGKSQLQGAQVKATDLRAAAALILAGLVADGKTSV
                     TELTHLDRGYVDLHGKLKQLGADIERIND"
     gene            1684..2124
                     /gene="fabZ"
                     /locus_tag="O0293_001271"
     CDS             1684..2124
                     /gene="fabZ"
                     /locus_tag="O0293_001271"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCY0280160.1"
                     /translation="METIFDYNQIKQIIPHRQPFLLIDKVVEYEEGQRCVAIKQVSGN
                     EPFFQGHFPEYAVMPGVLITEALAQTGAVAILNSEENKGKIALFAGIDKCRFKRQVVP
                     GDTLTLEVEITKIKGPIGKGNAKATVDGQLACSCELTFAIQDVK"
     gene            complement(2182..2622)
                     /locus_tag="O0293_001272"
     CDS             complement(2182..2622)
                     /locus_tag="O0293_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwpF-like family protein"
                     /protein_id="HCY0280161.1"
                     /translation="MKTFKAVRFQIVNEHGRIIEYELEDGVIINKEESGTGWLLEIVI
                     SNEHYETFKEYQDNEQLLDIRVVITRPANDPALFESTVKSIKNFKTTMSIVFECHIYT
                     LRQQYAESLLEQLIDDGLSGEELKKSFNRMMQSKPKLKDEKLEE"
     gene            2816..3163
                     /locus_tag="O0293_001273"
     CDS             2816..3163
                     /locus_tag="O0293_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002466750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCY0280162.1"
                     /translation="MLNKIVIVGRLTKDAQIFEKEDRKIATFCVATHRNYKDENGEIV
                     CDYLFCKAFGKLASNIEKYTNQGTLVGITGQMRSRKYDKDGQTHFVTELYVETIKFMS
                     PKSQNNEILSDSI"
     gene            3600..4295
                     /gene="sceD"
                     /locus_tag="O0293_001274"
     CDS             3600..4295
                     /gene="sceD"
                     /locus_tag="O0293_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase SceD"
                     /protein_id="HCY0280163.1"
                     /translation="MKKTLLASSLAVGLGIVAGNAGHEAHASEADLNKASLAQMAQSN
                     DQTLNQKPIEAGAYNYTFDYEGFTYHFESDGTHFAWNYHATGTNGADMSAQAPATNNV
                     APSAVQANQVQSQEVEAPQNAQTQQPQASTSNNSQVTATPTESKSSEGSSVNVNAHLK
                     QIAQRESGGNIHAVNPTSGAAGKYQFLQSTWDSVAPAKYKGVSPANAPESVQDAAAVK
                     LYNTGGAGHWVTA"
     regulatory      4499..4601
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4681..5370
                     /gene="tenA"
                     /locus_tag="O0293_001275"
     CDS             4681..5370
                     /gene="tenA"
                     /locus_tag="O0293_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HCY0280164.1"
                     /translation="MEFSQKLYQAAKPIINDIYEDDFIQKMLSGDIGADALRHYLKAD
                     AAYLKEFTNLYALLIPKMNSMNDVKFLVEQIEFMVEGEVLAHDILAQIVGESYEEIIK
                     TKVWPPSGDHYIKHMYFQAHSRENAIYTIAAMAPCPYIYAELAKRSQSDHKLNREKDT
                     AKWFDFYSTEMDDIINVFEALMNKLAESMSDKELEQVKQVFLESCIHERRFFNMAMTL
                     EQWEFGGKVND"
     gene            5363..6193
                     /gene="thiD"
                     /locus_tag="O0293_001276"
     CDS             5363..6193
                     /gene="thiD"
                     /locus_tag="O0293_001276"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCY0280165.1"
                     /translation="MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVA
                     QNTLGVQHIHNLNHQWVDEQLDSVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIP
                     YVIDPVMLAKSGDSLMDNDTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQ
                     AGRIFINEIGSKGVIIKGGHSNDTDIAKDYLFTNEGVQTFENERFKTKHTHGTGCTFS
                     AVITAELAKGRPLFEAVHKAKKFISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK
                     "
     gene            6177..6968
                     /gene="thiM"
                     /locus_tag="O0293_001277"
     CDS             6177..6968
                     /gene="thiM"
                     /locus_tag="O0293_001277"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCY0280166.1"
                     /translation="MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPE
                     EAEEFYKVAQALLINIGTLTAENEQDIIAIAQTANEAGLPIVFDPVAVGASTYRKQFC
                     KLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDANLDAVAIAKKAYATYKTAIVIT
                     GKEDVIVQDNKAFVLANGSPLLARVTGAGCLLGGVIAGFLFRETEPDIEALIEAVSVF
                     NIAAEVAAENENCGGPGTFSPLLLDTLYHLNETTYQQRIRIQEVE"
     gene            6970..7611
                     /locus_tag="O0293_001278"
     CDS             6970..7611
                     /locus_tag="O0293_001278"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCY0280167.1"
                     /translation="MFNQSYLNVYFICGTSDVPSHRTIHEVLEAALKAGITLFQFREK
                     GESALKGNDKLVLAKELQHLCHQYDVPFIVNDDVSLAKEINADGIHVGQDDAKVKEIA
                     QYFTDKIIGLSISDLDEYAKSDLTHVDYIGVGPIYPTPSKHDAHIPVGPEMIATFKEM
                     NPQLPIVAIGGINTNNVAPIVEAGANGISVISAISKSENIEKTVNRFKDFFNN"
     gene            7697..8569
                     /gene="yidC"
                     /locus_tag="O0293_001279"
     CDS             7697..8569
                     /gene="yidC"
                     /locus_tag="O0293_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCY0280168.1"
                     /translation="MKKKALLPLFLGIMVFLAGCDYSKPEKRSGFFYNTFVDPMKNVL
                     DWLGNNLLNDNYGLAIIILVLVIRIILLPFMLSNYKNSHMMRQKMKVAKPEVEKIQEK
                     VKRARTQEEKMAANQELMQVYKKYDMNPIKSMLGCLPMLIQLPIIMGLYFVLKDQLVD
                     GLFKYPHFLWFDLGRPDIWITIIAGVLYFIQAYVSSKTMPDEQRQMGYMMMVISPIMI
                     IWISLSSASALGLYWSVSAAFLVVQTHFANIYYEKVAKKEVQPFIEAYEREHNGGSNK
                     KGKNTQVVSKKKKK"
     gene            complement(9382..10029)
                     /locus_tag="O0293_001280"
     CDS             complement(9382..10029)
                     /locus_tag="O0293_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCY0280169.1"
                     /translation="MQQSDVISAAKKYMESIHQNDYTGHDIAHVYRVTALAKSIAENE
                     GVNDTLVIELACLLHDTVDEKVVDANKQYVELKSFLSSLSLSTEDQEHILFIINNMSY
                     RNGKNDHVTLSLEGQIVRDADRLDAIGAIGVARTFQFAGHFGEPMWTEHMSLDKINDD
                     LVEQLPPSAIKHFFEKLLKLESLMHTDTAKMIAKERHDFMMMYLKQFFTEWNCHD"
     gene            complement(10057..11541)
                     /gene="cls"
                     /locus_tag="O0293_001281"
     CDS             complement(10057..11541)
                     /gene="cls"
                     /locus_tag="O0293_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCY0280170.1"
                     /translation="MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANS
                     IWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDKEDKKGLELIVDEQLAALKNENF
                     SNSNYQIVKFKEMIQMLLYNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQ
                     YYIIQNDELGRTILNELGKKAEQGVEVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFF
                     PSKLPLINLRMNNRNHRKIVVIDGQIGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGD
                     AVNALQLRFILDWNSQATRDHISYDDRYFPDVNSGGTIGVQIASSGPDEEWEQIKYGY
                     LKMISSAKKSIYIQSPYFIPDQAFLDSIKIAALGGVDVNIMIPNKPDHPFVFWATLKN
                     AASLLDAGVKVFHYDNGFLHSKTLVIDDEIASVGTANMDHRSFTLNFEVNAFIYDQQI
                     AKKLKQAFIDDLAVSSELTKARYAKRSLWIKFKEGISQLLSPIL"
     gene            11707..12000
                     /gene="csoR"
                     /locus_tag="O0293_001282"
     CDS             11707..12000
                     /gene="csoR"
                     /locus_tag="O0293_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-sensing transcriptional repressor CsoR"
                     /protein_id="HCY0280171.1"
                     /translation="MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDV
                     LTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQEEAMEELLVTFQKLIKD"
     gene            12012..12221
                     /locus_tag="O0293_001283"
     CDS             12012..12221
                     /locus_tag="O0293_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HCY0280172.1"
                     /translation="MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVT
                     VSYETPANLNSIEKEIYDEGYKIVF"
     gene            12349..12486
                     /locus_tag="O0293_001284"
     CDS             12349..12486
                     /locus_tag="O0293_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280173.1"
                     /translation="MKRPEKIQNVVKLLSSLGVNIKKTKSRLDIINTLPASNKVSHEL
                     K"
     gene            complement(12750..13952)
                     /locus_tag="O0293_001285"
     CDS             complement(12750..13952)
                     /locus_tag="O0293_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HCY0280174.1"
                     /translation="MNYSSRQQPDKHWLRKVDWVLVATIAVLAIFSVLLINSAMGGGQ
                     YSANFGIRQIFYYILGAIFAGIIMFISPKKIKHYTYLLYFLICLLLIGLLVIPESPIT
                     PIINGAKSWYTFGPISIQPSEFMKIILILALARVVSRHNQFTFNKSFQSDLLLFFKII
                     GVSLVPSILILLQNDLGTTLVLAAIIAGVMLVSGITWRILAPIFITGIVGAMTVILGI
                     LYAPALIENLLGVQLYQMGRINSWLDPYTYSSGDGYHLTESLKAIGSGQLLGKGYNHG
                     EVYIPENHTDFIFSVIGEELGFIGSVILILIFLFLIFHLIRLAAKIEDQFNKIFIVGF
                     VTLLVFHILQNIGMTIQLLPITGIPLPFISYGGSALWSMMTGIGIVLSIYYHEPKRYV
                     DLYHPKSN"
BASE COUNT         5057 a         2228 c         2501 g         4372 t
ORIGIN      
        1 tcatgtacaa tgtaatacag taatcttatg aggtgataaa atggattata tcggacaata
       61 tgcagttatc catttagtgt tacatgttgt atgtatttgt attgcctatt gggctttaca
      121 atcaattaga ttagatcaat tttttaaaaa aggatacgcc actcaattac aagtgtgtat
      181 gatatttgtt gctattttat taggcactgc agtaagcaat tttattgtag atttgttaca
      241 atactcgacg caggtaaaat atttaataaa ataagtctaa ctctatgatt tgtaatcaaa
      301 actagatata attaaataat gacttaaaat aattttaaaa tagggaaatg taaagtaata
      361 ggagttctaa gtggaggatt tacgatggat aaaatagtaa tcaaaggtgg aaataaatta
      421 acgggtgaag ttaaagtaga aggtgctaaa aatgcagtat taccaatatt gacagcatct
      481 ttattagctt ctgataaacc gagcaaatta gttaatgttc cagctttaag tgatgtagaa
      541 acaataaata atgtattaac aactttaaat gctgacgtta catacaaaaa ggacgaaaat
      601 gctgttgtcg ttgatgcaac aaagactcta aatgaagagg caccatatga atatgttagt
      661 aaaatgcgtg caagtatttt agttatggga cctcttttag caagactagg acatgctatt
      721 gttgcattgc ctggtggttg tgcaattgga agtagaccga ttgagcaaca cattaaaggt
      781 tttgaagctt taggcgcaga aattcatctt gaaaatggta atatttatgc taatgctaaa
      841 gatggattaa aaggtacatc aattcattta gattttccaa gtgtaggagc aacacaaaat
      901 attattatgg cagcatcatt agctaagggt aagactttaa ttgaaaatgc agctaaagaa
      961 cctgaaattg tcgatttagc aaactacatt aatgaaatgg gtggtagaat tactggtgct
     1021 ggtacagaca caattacaat caatggtgta gaatcattac atggtgtaga acatgctatc
     1081 attccagata gaattgaagc aggcacatta ctaatcgctg gtgctataac gcgtggtgat
     1141 atttttgtac gtgatgcaat caaagaacat atggcgagtt tagtctataa actagaagaa
     1201 atgggcgttg aattggacta tcaagaagat ggtattcgtg tacgtgctga aggggaatta
     1261 caacctgtag acatcaaaac tctaccacat cctggattcc cgactgatat gcaatcacaa
     1321 atgatggcat tgttattaac ggcaaatggt cataaagtcg taaccgaaac tgtttttgaa
     1381 aaccgtttta tgcatgttgc agagttcaaa cgtatgaatg ctaatatcaa tgtagaaggt
     1441 cgtagtgcta aacttgaagg taaaagtcaa ttgcaaggtg cacaagttaa agcgactgat
     1501 ttaagagcag cagccgcctt aattttagct ggattagttg ctgatggtaa aacaagcgtt
     1561 actgaattaa cgcacctaga tagaggctat gttgacttac acggtaaatt gaagcaatta
     1621 ggtgcagaca ttgaacgtat taacgattaa ttcagtaaat taatataatg gaggatttca
     1681 accatggaaa caatttttga ttataaccaa attaaacaaa ttatacctca cagacagcca
     1741 tttttattaa ttgataaagt agttgaatat gaagaaggtc aacgttgtgt ggctattaaa
     1801 caagtatcag gaaacgaacc attctttcaa gggcattttc ctgagtatgc ggtaatgcca
     1861 ggcgtattaa ttactgaagc gttagctcaa acaggtgcgg tagctatttt aaatagtgaa
     1921 gaaaataaag gtaaaatcgc tttatttgct ggtattgata aatgtcgttt taaacgtcaa
     1981 gtagtacctg gtgatacttt aacgttggaa gtagaaatca ctaaaattaa aggaccaata
     2041 ggtaaaggta atgctaaagc tactgtcgat ggtcaacttg cttgtagttg tgaacttaca
     2101 tttgcaattc aagatgtaaa ataaaacaaa aaaacattca aagatttaat gtgttggcat
     2161 aatctttgaa tgttttttat tttactcttc taatttttca tcctttaact ttggtttaga
     2221 ctgcatcatt cgattaaatg atttttttaa ttcttcacca gataatccat catcaataag
     2281 ttgttctaat aaactttcag catactgttg acgtaaagtg taaatatgac attcaaatac
     2341 gatagacatt gttgttttaa aatttttaat acttttcaca gtagattcaa ataaagctgg
     2401 atcattggct ggacgcgtga tgacgacacg aatatccaat aactgttcat tgtcttgata
     2461 ttccttaaat gtttcataat gctcattaga aatgacaatt tcaagcaacc aacccgtgcc
     2521 actttcctct ttattaatta ttacgccatc ttctaattca tattctataa ttctgccgtg
     2581 ttcattcaca atttgaaacc ttactgcttt aaaagttttc atattcccac atcccattag
     2641 tttaatattt atgatttttg aattatttag aagatttaag gtcatttcat gactaatttc
     2701 tttaaatata acaaataacg agatgctata gctattaatt atataaaatt tgccattttg
     2761 accattttta agctagtaat taagatatag gcataggagg tgaatcacac actacatgct
     2821 aaataaaatc gtaattgtcg ggagactgac gaaagacgca caaatatttg aaaaggagga
     2881 tagaaaaatt gcaacgtttt gtgttgcaac gcaccgaaat tataaagatg aaaatggaga
     2941 aatcgtctgt gattacttat tctgtaaagc atttggcaag ttagcttcta atatagaaaa
     3001 atatactaat caaggtacat tggttggtat aactggtcaa atgagatcaa gaaagtatga
     3061 taaagacgga caaacacact ttgtcactga attatatgtt gaaacaataa aatttatgtc
     3121 ccctaaatcc caaaataatg aaattctctc agatagtatt tagatattga ctctcaaaat
     3181 atagataatc atgacttatt agaaatttaa atccaattat cctaaacatc cttaatatac
     3241 atttaatcca tcctaaattt tttatagatc taattaactt gaaatatact cacttaaaaa
     3301 cgacttacat gactagtact gttgtgtagg tcgtcttttt gtgtagtttt tctaatagaa
     3361 aatagacata gattgcaaaa cgattacata aaaaattgca aataaaaaaa catttttact
     3421 gtcatgacaa ttctcgatag aatcatatct gaaattatgg gggatttcgt tgtaatcact
     3481 gtgtaaaaaa caaggctatt tttttatttt gttgtaacct gcacggattt tatagatgtt
     3541 acagtagtct ctgtaaaact tttagagaaa caaattactt gtaggaggat tttaagatta
     3601 tgaagaaaac attactcgca tcatcattag cagtaggttt aggaatcgta gcaggaaatg
     3661 caggtcacga agcccatgca agtgaagcgg acttaaataa agcatcttta gcgcaaatgg
     3721 cgcaatcaaa tgatcaaaca ttaaatcaaa aaccaattga agctggggct tataattata
     3781 catttgacta tgaagggttt acttatcact ttgaatcaga tggtacacac tttgcttgga
     3841 attaccatgc aacaggtact aatggagcag acatgagtgc acaagcacct gcaactaata
     3901 atgttgcacc atcagctgtt caagctaatc aagtacaatc acaagaagtt gaagcaccac
     3961 aaaatgctca aactcaacaa ccacaagcat caacatcaaa caattcacaa gttactgcaa
     4021 caccaactga atcaaaatca tcagaaggtt catcagtaaa tgtgaatgct catctaaaac
     4081 aaattgctca acgtgaatca ggtggcaata ttcatgctgt aaatccaaca tcaggtgcag
     4141 ctggtaagta tcaattctta caatcaactt gggattcagt agcacctgct aaatataaag
     4201 gtgtatcacc agcaaatgct cctgaaagtg ttcaagatgc cgcagcagta aaattatata
     4261 acactggtgg cgctggacat tgggttactg cataagccat ttatgcatag ctaattaata
     4321 gttatataag taacttttag attggaatat atcggggaat tctcgatatg ttccggtttt
     4381 tttatttcgc aaatatatgt cattatagaa ttattattta aagataataa tacaactgag
     4441 caacaataat tgaaaatgtt gaaaccgttt gctaagatta atttgcaatt gaatactaag
     4501 gaactactag gggagcctaa tgatatggct gagatgaatt gttcagaccc ttatgacctg
     4561 atttggttag taccaacgta ggaaagtagt tattgaagtg atggggctag tatagcaaca
     4621 ttgccatcaa actactttca tgttggaagt agttttttta tttagggagg attttttaca
     4681 atggaatttt cacaaaaatt gtaccaagct gcaaagccaa tcattaatga tatttatgag
     4741 gatgacttta ttcagaagat gttatccgga gatataggag ctgacgcatt acgccattac
     4801 ttaaaagctg atgctgcgta tttaaaagaa tttacaaact tatatgcttt gttaattcca
     4861 aaaatgaata gcatgaatga cgtaaaattt ttagtagagc aaatagaatt tatggttgaa
     4921 ggtgaagtac ttgcgcatga tattttagcg caaattgttg gtgaatcata cgaagaaatc
     4981 attaaaacaa aagtttggcc tccaagtggc gaccattaca taaaacatat gtatttccaa
     5041 gcgcatagtc gtgaaaatgc tatttataca attgctgcaa tggcaccttg tccatatatt
     5101 tatgcagaat tagctaagcg ctctcaaagt gatcataagc ttaatagaga aaaagatacg
     5161 gcaaaatggt ttgattttta tagtactgaa atggatgaca taattaacgt atttgaggca
     5221 ttaatgaata aattagccga atcaatgtct gacaaggaat tggaacaagt aaagcaagta
     5281 tttttagaaa gttgcataca tgagcgtcga tttttcaata tggctatgac actagaacaa
     5341 tgggaatttg gaggaaaagt aaatgattaa acctaaaata gcattaacca ttgcaggtac
     5401 tgatccaaca ggtggtgccg gcgtaatggc tgatttaaaa tcatttcatt catgtggtgt
     5461 atatggtatg ggcgtcgtta caagtattgt tgctcaaaat acattgggcg tacaacatat
     5521 tcataattta aatcatcaat gggtagatga acaacttgat agtgtcttca atgatacctt
     5581 acctcatgct attaaaacgg ggatgattgc tacagcagat actatggaaa cgattcgtca
     5641 ttatttaatg caacatgaat ctattccata tgtaattgat cctgttatgt tggcgaaaag
     5701 cggtgattca ttaatggata atgacacaaa gcaaaacttg cagcatacgt tattgccatt
     5761 agctgacgta gtaacaccga atttaccaga agctgaagaa ataacgggac taaccattga
     5821 tagtgaagaa aaaattatgc aggctggccg catctttatt aatgagattg gtagtaaagg
     5881 tgtcatcatt aaaggcggtc attcaaatga tactgatata gcaaaagatt atttatttac
     5941 taacgaaggt gttcaaacat ttgaaaatga acgatttaaa acaaaacata cgcatggaac
     6001 agggtgtaca ttttcagcag ttataacggc agaacttgca aaaggtagac cattatttga
     6061 ggctgtacac aaggctaaaa agtttatttc aatgagtata caatatacgc ctgaaatcgg
     6121 ccgtggtaga ggtccagtga atcattttgc atatttaaag aaagagggat tagacgatga
     6181 attatctaaa taaaatacgt attgaaaacc cattaacaat ttgttataca aacgatgtag
     6241 ttaaaaattt tacagcgaat ggtttattaa gtattggtgc tagccctgca atgagtgaag
     6301 ctcccgaaga agctgaagaa ttttacaaag ttgcacaagc gctattaatc aatatcggta
     6361 ctttaacagc agaaaatgaa caagatatta ttgcgattgc tcaaacggca aatgaggcag
     6421 gcttacctat tgtatttgac cctgtagctg ttggtgcttc tacatatcga aagcaatttt
     6481 gtaaattatt attgaaatca gcgaaagtat cagtaattaa aggcaatgca tctgaaatat
     6541 tagcgttgat tgatgataca gcaactatga aaggtacaga tagtgatgct aatcttgatg
     6601 cggttgcaat agcgaaaaag gcttacgcaa catataaaac tgcaatagta atcacaggta
     6661 aagaggacgt tattgttcaa gataataaag ccttcgtatt agctaatgga tctccattat
     6721 tagcacgagt aactggagct ggttgtttat taggaggcgt tattgctgga tttttattta
     6781 gagaaacaga accagacata gaagcgttaa ttgaagcggt aagcgtattt aatattgctg
     6841 ctgaggtagc tgctgaaaat gaaaattgtg gtggtcctgg tacgttttca ccattgttgc
     6901 ttgatacgtt atatcattta aatgaaacaa cctatcaaca acggattcgt attcaagagg
     6961 tggaataata tgtttaacca atcgtatcta aatgtgtatt ttatttgtgg gacctccgat
     7021 gttccgagtc atcgaactat tcatgaagta ttagaagcag cattaaaggc agggattaca
     7081 cttttccaat ttcgagaaaa gggtgaatca gctttaaaag gaaacgataa acttgtatta
     7141 gctaaagaat tacagcatct ctgtcatcaa tatgatgtgc cttttatcgt taatgatgat
     7201 gtatccttgg caaaagaaat taacgcggat ggtatacatg tcggtcaaga tgatgcgaaa
     7261 gtcaaagaga tagcacaata tttcactgat aaaattattg gacttagtat tagtgattta
     7321 gatgagtatg caaaatctga tttaactcat gttgattata taggtgttgg accaatctat
     7381 cctacgccgt cgaagcatga cgcgcatata ccagtaggtc cagaaatgat tgcaacgttt
     7441 aaagaaatga atccgcaact accgattgta gctattggcg gtatcaatac taataatgtg
     7501 gcgccgattg tagaagcagg tgccaatgga atttcagtca tttcagccat ttctaaaagt
     7561 gaaaatattg aaaagactgt taatcgattc aaagattttt ttaataatta gttaaatttt
     7621 tccacttatt gtttcatacg tactcattaa tgtgatagaa taaatcctat gtgaatataa
     7681 tgatagaggt ggaaaaatga agaaaaaagc gttactacca ttatttttag gtattatggt
     7741 ctttttggct ggttgtgact attctaaacc tgaaaaacgt agtgggtttt tctacaatac
     7801 attcgtagat ccaatgaaaa atgtattgga ttggttggga aataacttat taaacgacaa
     7861 ttatggttta gctattatta tccttgtatt ggtaattcgt attattttat taccattcat
     7921 gttgtcaaac tataaaaata gtcatatgat gcgtcaaaaa atgaaagttg caaagccaga
     7981 agttgaaaaa attcaagaaa aagtgaaacg tgcgcgtaca caagaagaaa aaatggctgc
     8041 aaaccaagaa ttaatgcaag tatataaaaa gtatgacatg aacccgatta agagtatgtt
     8101 gggttgttta ccaatgctaa tccaattacc aatcatcatg ggattatact ttgtacttaa
     8161 agaccaactt gtagatggtt tgtttaaata tccacacttc ttatggttcg atttaggacg
     8221 tcctgatatt tggattacaa ttattgccgg tgttttatac tttatccaag catatgtatc
     8281 aagtaaaacg atgccagacg aacaacgtca aatgggttac atgatgatgg tcatttcacc
     8341 aattatgatt atctggattt cattaagctc agcatcagca cttggtttgt actggtcagt
     8401 cagtgcggcg ttccttgtag ttcaaacaca ctttgcgaac atttattatg aaaaagtcgc
     8461 taaaaaagaa gtacaacctt tcattgaagc gtatgaaaga gagcacaacg gcggcagcaa
     8521 taaaaaaggc aaaaacacac aagttgtgtc taaaaagaaa aagaaataac cttagcaagt
     8581 ccaaataatg tgcaccctaa aagttgaaga taattttaac tttatagggc gcacattatt
     8641 tttcgcttca atagcgaaac ttagtgtcac attatacaat atgtgataaa tagaaacagc
     8701 agtaagatga tttcaaatgt gaaagtcatc ctactgctgt ttttatttga aataatatta
     8761 acgagttttc agtatgtaaa aagtggttaa taacagagtt gtcattcttc atcgcaatgt
     8821 catcttgaca caatgattca tcagtgtagc agtatatgtc gcaaatttcc acagcaattc
     8881 acttatacat gtaacagtgt gaaataataa cgtcacaacg gcataataca cattgcaacg
     8941 tcccaacaac atatcataga atcgtaacgg catgacatca acaccaaacc tggctataac
     9001 accataacag cgcaacgcca catcatagca ctacatcaag tcgcatcgta gcaccagcac
     9061 atcaaagtaa catggcatca acatcaaacc ggtctataac accataacac cgcaatgcca
     9121 catcatagca ctacatcaag tcgcatcgtg gtaccagcac atcaaagtaa catggcatca
     9181 acgccaaacc tgtctataac accataacac cgcaatgcca catcatagca ctacatcaag
     9241 acgcatcgtg gtaccagcac atcaaagtag catgacatgt caccaagctc ccacaacaac
     9301 acatcaaagt aacatggcat gacaccaagc ttccacaaca acacgccatt acatcgcatc
     9361 atcatactac aacttcaatg tctagtcgtg acaattccat tccgtaaaaa actgtttcaa
     9421 gtacatcatc ataaagtcgt gacgttcttt agcaatcatc ttcgccgtat ctgtatgcat
     9481 taaagattct aacttaagta atttttcaaa gaaatgttta attgcagatg gtggcaactg
     9541 ttcaactaaa tcatcattaa tcttatctag tgacatatgt tctgtccaca taggttcacc
     9601 aaagtgtcct gcaaattgaa atgttcgtgc aacacctata gcgcctatag catcaagacg
     9661 atctgcatcc ctgacaattt gaccttctaa agataaagtg acatgatcat ttttgccatt
     9721 gcgatagctc atattattaa taataaataa aatgtgctct tgatcttcgg ttgatagtga
     9781 taaagaagat aaaaatgact tcaattcaac atattgtttg ttagcatcta caactttttc
     9841 gtcaacggta tcatgaagca aacatgcgag ttcaatgact aaagtatcat taacaccttc
     9901 attttcagcg attgatttag ctaaagcagt gacacgatat acatgcgcaa tatcatggcc
     9961 tgtataatca ttttgatgaa tagattccat atattttttg gcagcactaa tgacgtctga
    10021 ttgttgcatt aaagttacac tcctcatatt tctattttat aagataggtg acaataattg
    10081 tgaaatacct tctttaaatt taatccaaag acttcgctta gcataacgtg cttttgttaa
    10141 ttcagaagat actgctaaat catctataaa agcttgtttt aattttttgg caatttgttg
    10201 gtcataaata aaagcgttga cttcgaaatt caatgtgaaa ctgcgatggt ccatattagc
    10261 tgttcccaca cttgcaattt catcatctat aacaagtgtt tttgagtgta aaaagccatt
    10321 gtcgtagtga aatactttaa caccggcatc taataaggat gctgcatttt ttaaagtagc
    10381 ccaaaaaaca aacggatggt caggtttatt aggaatcatg atattgacat caacaccacc
    10441 taatgccgca attttaatag aatctaaaaa ggcttgatca ggtatgaaat agggagattg
    10501 aatataaatc gattttttag cagatgaaat cattttcaaa tagccgtatt taatctgttc
    10561 ccattcttcg tcaggaccac tagaagctat ttgaacgcca attgttccac cagaatttac
    10621 atctgggaaa taacgatcat cataggagat gtggtcacgt gtggcttgtg aattccaatc
    10681 tagaataaat cgtaattgca atgcattcac tgcatccccg acaattcgta aatgcgtatc
    10741 tcgccaatag ccgaattttt ttgatttacc taagtactca tcaccaacat taaaaccacc
    10801 aacatatcca atttgcccat ctattacaac aatttttcga tggtttcgat tgttcatacg
    10861 caagttaatt aaaggtaatt ttgatgggaa aaatgcttca gcatgtccac ctttattgcg
    10921 aaacgggcgt aagccttttt tacgcagtcc acgagaaccc atgtcatcat aaagaatttt
    10981 aacttctaca ccttgttccg cttttttacc aagttcattt aaaatggtac gacctaattc
    11041 atcattttga ataatatagt actgaaaatg aatataatca gtagcattac ggatgtcttg
    11101 tattaggtca tcaaattttt cttggccgtc tgtgtatatt tttaaatcgt tgtctgttgt
    11161 taaaaatgct gcgttattat ataacaacat ttgaatcatt tctttaaatt ttacaatttg
    11221 ataattggaa tttgaaaagt tttcattttt taaagcagct aattgctcat caacgattaa
    11281 ctctaatcct tttttatctt ccttatcaat tttgaaaatt tggtcacgtt gaatttgtcg
    11341 tcctaatagt aagtataaaa tgaagccgaa taaaggcaag aaaactaaga ctagtaacca
    11401 agcccagata gaattggcag aacgtctttc catgaaaata atggtaaagg cgaataataa
    11461 gtttaaaata aatgcaccaa taaatattga atttaaaata atattagaat gcttgagtgc
    11521 aatggataat aactctatca tttgaaacct cccatcgaaa atcttaaacg agtagtatat
    11581 cttattaatt gtatcatgaa gcaatttaaa tatcatgaca atttagtttg aaaaacacac
    11641 tttgtgttga cgttgtgttt tatgggatat agtatagtaa ggaatgtaaa tgaaggagtg
    11701 aatgctatga ctgaacaaga taatgcacat cattctgaac aaataaaaac gaatcttaaa
    11761 tcacgtttaa atcgaattga aggacaagtg agagcgatta atcgcatgat tgaagaagat
    11821 gtctattgtg atgatgtcct tacgcaaata agagcgacac gttcggcgtt aaacagtgtt
    11881 gcgataaagt tattagaaca acatatgaaa agttgtatta tgaataaagt taatcaaggt
    11941 gctcaggaag aggcaatgga agagttatta gtgacttttc aaaaattgat taaagactaa
    12001 aggagtttaa gatgatacat caaaatacga tttacacagc gggaattgaa acagaagaac
    12061 aagtaagtca attgacagaa cgcatttcaa atatgatagg tgttcatcaa gtgaatatta
    12121 atataataga tggtcaagta actgtatcgt atgagacacc agcaaatttg aatagtattg
    12181 aaaaagaaat ctatgatgaa ggatacaaaa ttgtatttta gggtataatg tagagtgcgc
    12241 tatagatttt aattttgaaa ataaattaaa aattttgtaa atgatgtagt aaaggtatgt
    12301 cgaatagggt aagataatat tgaaagcgat ccaaacgagg tgataataat gaaacgtcct
    12361 gaaaagattc aaaatgtagt caaactattg tcatcattag gtgtgaatat taaaaaaact
    12421 aaatctcgtt tagacattat taatactttg ccagcatcta ataaagtaag tcacgaatta
    12481 aaataattga ctgtgattgt gaatcacaca ccgttaaagc tatcgtcgga ctattcgatg
    12541 atagcttttt tgactggatt tactaaaatg aaagcaagta ctatataaca aaatccctct
    12601 ttagagtgta cattttacaa tgtgtacttc aaagagggat tgttattatc gagtaatatc
    12661 tatatatgac attttaaatc gacgacgtaa atagactata taacgtcatc ttgaaaagtt
    12721 atgatatttg aaactcaaaa tagtttaaat taattacttt ttggatggta taaatcgaca
    12781 tatcgttttg gttcatgata ataaattgat aagactatac ctattccagt catcatactc
    12841 catagcgcac taccaccata actaataaat ggtaatggaa taccagtgat tggtaacaac
    12901 tgaattgtca taccaatatt ttgtaaaata tggaacacaa gtaaagtgac gaaaccaacg
    12961 ataaagattt tgttaaattg atcttcaatt ttcgcagcta atcttattag atggaagatt
    13021 aaaaataaaa atattaagat caatatgaca gaaccgataa agccaagttc ctctccaatc
    13081 actgaaaaga taaagtcagt atgattttca ggtatataaa cttcaccgtg attgtatcct
    13141 ttacctagta actgtccaga accgatagct ttaagtgatt cagttaaatg atagccatca
    13201 ccactactat atgtataggg gtcaagccat gaattgattc gtcccatttg atacagttgg
    13261 acacctaata aattttcaat taatgcgggt gcatatagaa tacctaaaat gactgtcatt
    13321 gcaccaacaa tacctgtaat aaagataggt gctaagatac gccatgttat accacttact
    13381 aacatcacac ctgcaataat agcagctaat actaatgtag ttcctaggtc attttgcagt
    13441 aatattaaaa tacttggtac taacgagaca ccaataattt tgaaaaataa taacaaatca
    13501 ctttggaatg atttattgaa tgtgaattga ttatgtctag aaacgacacg cgctaatgct
    13561 aaaattaaaa taattttcat gaattcagat ggctgaatac tgatagggcc aaacgtgtac
    13621 caacttttgg caccattgat aataggtgta ataggtgact caggaataac gagcaagcct
    13681 attaataata gacagattaa gaaatacaat aaatatgtat aatgtttaat ctttttaggt
    13741 gaaataaaca tgatgatacc tgcaaaaatt gcacctaaaa tgtaataaaa aatttgtctg
    13801 ataccgaaat tagcactgta ttgaccaccg cccattgccg agttaataag cagaacactg
    13861 aaaattgcta aaacagctat agtggctact aatacccagt ctactttgcg aagccaatgc
    13921 ttatccggct gttgacgaga tgaataattc attgcaaact ccttttatac tcactaatgt
    13981 ttatatcaat tttacatgac tttttaaaaa ttagctagaa tatcacagtg atatcagcta
    14041 tagatttcaa tttgaattag gaataaaata gaagggaata ttgttctgat tataaatgaa
    14101 tcaacataga tacagacaca taagtcctcg tttttaaaat gcaaaatagc attaaaat
//
LOCUS       DAKTIO010000056        14018 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.056, whole genome shotgun sequence.
ACCESSION   DAKTIO010000056 DAKTIO010000000
VERSION     DAKTIO010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14018
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.056"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            6..2624
                     /gene="mutS"
                     /locus_tag="O0293_001286"
     CDS             6..2624
                     /gene="mutS"
                     /locus_tag="O0293_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCY0280175.1"
                     /translation="MSNVTPMMQQYLKIKSEYQDCLLFFRLGDFYEMFYEDAKEASRV
                     LEITLTKRDAKKENPIPMCGVPYHSADSYIDTLVNNGYKVAICEQMEDPKQTKGMVRR
                     EVVRIVTPGTVMEQGGVDDKQNNYILSFVMNQPEIALSYCDVSTGELKVTHFNDEATL
                     LNEITTINPNEVVINDNISDNLKRQINMVTETITVRETLSSEIYSVNQTEHKLMYQAT
                     QLLLDYIHHTQKRDLSHIEDVVQYAAIDYMKMDFYAKRNLELTESIRLKSKKGTLLWL
                     MDETKTPMGARRLKQWIDRPLISKEQIEARLDIVDEFSAHFIERDTLRTYLNQVYDIE
                     RLVGRVSYGNVNARDLIQLKHSISEIPNIKALLNSMNQNTLVQVNQLEPLDDLLDILE
                     QSLVEEPPISVKDGGLFKVGFNTQLDEYLEASKNGKTWLAELQAKERQRTGIKSLKIS
                     FNKVFGYFIEITRANLQNFEPSEFGYMRKQTLSNAERFITDELKEKEDIILGAEDKAI
                     ELEYQLFVQLREEVKKYTERLQQQAKIISELDCLQSFAEIAQKYNYTRPSFSENKTLE
                     LVESRHPVVERVMDYNDYVPNNCRLDNETFIYLITGPNMSGKSTYMRQVAIISIMAQM
                     GAYVPCKEAVLPIFDQIFTRIGAADDLVSGKSTFMVEMLEAQKALTYATEDSLIIFDE
                     IGRGTSTYDGLALAQAMIEYVAETSHAKTLFSTHYHELTTLDQALPSLKNVHVAANEY
                     KGELIFLHKVKDGAVDDSYGIQVAKLADLPEKVISRAQVILSEFEASAGKKSSISNLK
                     MVENEPEINQENLNLSVEETTDTLSQKDFEQASFDLFENDQESEIELQIKNLNLSNMT
                     PIEALVKLSELQNQLK"
     gene            2637..4646
                     /gene="mutL"
                     /locus_tag="O0293_001287"
     CDS             2637..4646
                     /gene="mutL"
                     /locus_tag="O0293_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCY0280176.1"
                     /translation="MGKIKELQTSLANKIAAGEVVERPSSVVKELLENAIDAGATEIS
                     IEVEESGVQSIRVVDNGSGIEAEDLGLVFHRHATSKLDQDEDLFHIRTLGFRGEALAS
                     ISSVAKVTLKTCTDNANGNEIYVENGEILNHKPAKAKKGTDILVESLFYNTPARLKYI
                     KSLYTELGKITDIVNRMAMSHPDIRIALISDGKTMLSTNGSGRTNEVMAEIYGMKVAR
                     DLVHISGDTSDYHIEGFVAKPEHSRSNKHYISIFINGRYIKNFMLNKAILEGYHTLLT
                     IGRFPICYINIEMDPILVDVNVHPTKLEVRLSKEEQLYQLIVSKIQEAFKDRILIPKN
                     NLDYVPKKNKVLHSFEQQKIEFEQRQNTENNQEKTFSSEESNSKPFMEENQNDEIVIK
                     EDSYNPFVTKTSESLIADDESSGYNNTREKDEDYFKKQQEILQEMDQTFDSNDGTTVQ
                     NYENKASDDYYDVNDIKGTKSKDPKRRIPYMEIVGQVHGTYIIAQNEFGMYMIDQHAA
                     QERIKYEYFRDKIGEVTNEVQDLLIPLTFHFSKDEQLVIDQYKNELQQVGIMLEHFGG
                     HDYIVSSYPVWFPKDEVEEIIKDMIELILEEKKVDIKKLREDVAIMMSCKKSIKANHY
                     LQKHEMSDLIDQLREAEDPFTCPHGRPIIINFSKYELEKLFKRVM"
     gene            4652..5194
                     /locus_tag="O0293_001288"
     CDS             4652..5194
                     /locus_tag="O0293_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCY0280177.1"
                     /translation="MNQVNNNILPAIRNIKDLEKLIKTDYKMCVLLDMHIGHIKSIME
                     LLKQNHIECFIHIDLIKGLSHDEFASEFIIQQYKPKGIVSTKSKVIKKAKSLNTLTIF
                     RVFIIDSQALKRSIDLIKKVEPDFVEVLPGVASKAIHHIQKETNTQVIAGGLINTIDE
                     VNEAVKNGAKYVTTSYDKLW"
     gene            5659..6477
                     /locus_tag="O0293_001289"
     CDS             5659..6477
                     /locus_tag="O0293_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCY0280178.1"
                     /translation="MNVYLAEFLGTAILILFGGGVCANVNLKRSAANGADWIVITAGW
                     GLAVTMGVFAVGQFSGAHLNPAVSLALALDGSFDWSLVPGYIVAQMLGAIVGATIVWL
                     MYLPHWKATEEAGAKLGVFSTAPAIKNYFANFLSEIIGTMALTLGILFIGVNKIADGL
                     NPLIVGALIVAIGLSLGGATGYAINPARDLGPRIAHAILPIAGKGGSNWSYAIVPILG
                     PIAGGLLGAVVYAVFYKHTFNIGCAIAIVVVIITLILGYILNKSSKKGDIESIY"
     gene            6606..8102
                     /gene="glpK"
                     /locus_tag="O0293_001290"
     CDS             6606..8102
                     /gene="glpK"
                     /locus_tag="O0293_001290"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCY0280179.1"
                     /translation="MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWV
                     EHDANEIWTSVLAVMTEVINENDVRADQIAGIGITNQRETTVVWDKHTGRPIYHAIVW
                     QSRQTQSICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGDLL
                     FGTIDTWLVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKA
                     SSEVYGKTIDYHFYGQEVPIAGVAGDQQAALFGQACFERGDVKNTYGTGGFMLMNTGD
                     KAVKSESGLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLRMINSAPQSESYATR
                     VDSTEGVYVVPAFVGLGTPYWDSEARGAIFGLTRGTEKEHFIRATLESLCYQTRDVME
                     AMSKDSGIDVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAFLAGLAV
                     GFWESKDDIAKNWKLEEKFDPKMDEGEREKLYRGWKKAVEATQVFKTE"
     gene            8260..9933
                     /locus_tag="O0293_001291"
     CDS             8260..9933
                     /locus_tag="O0293_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HCY0280180.1"
                     /translation="MALSTFKREHIKKNLRNDEYDLVIIGGGITGAGIALDASERGMK
                     VALVEMQDFAQGTSSRSTKLVHGGLRYLKQFQIGVVAETGKERAIVYENGPHVTTPEW
                     MLLPMHKGGTFGKFSTSIGLGMYDRLAGVKKSERKKMLSKKETLAKEPLVKKEGLKGG
                     GYYVEYRTDDARLTIEVMKRAAEKGAEIINYTKSEHFTYDKNQQVNGVKVIDKLTNEN
                     YTIKAKKVVNAAGPWVDDVRSGDYARNNKKLRLTKGVHVVIDQSKFPLGQAVYFDTEK
                     DGRMIFAIPREGKAYVGTTDTFYDNIKSSPLTTQEDRDYLIDAINYMFPSVNVTDEDI
                     ESTWAGIRPLIYEEGKDPSEISRKDEIWEGKSGLLTIAGGKLTGYRHMAQDIVDLVSK
                     RLKKDYGLTFSPCNTKGLAISGGDVGGSKNFDAFVEQKVDVAKGFGIDEDVARRLASK
                     YGSNVDELFNIAQTSQYHDSKLPLEIYVELVYSIQQEMVYKPNDFLVRRSGKMYFNIK
                     DVLDYKDAVIDIMADMLDYSPAQIEAYTEEVEQAIKEAQHGNNQPAVKE"
     gene            10083..10997
                     /locus_tag="O0293_001292"
     CDS             10083..10997
                     /locus_tag="O0293_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0280181.1"
                     /translation="MTEKQFKLTVQDNTNIEVKVNFTDVDSKGIIHIFHGMAEHMERY
                     DKLAHALSKHGFDVIRHNHRGHGINIDESTRGHYDDMKRVIGDAFEVAQTVRGNVDKP
                     YIIIGHSMGSVIARLFVETYPQYVDGLILSGTGMYSLWKGLPTVKVLQLITKIYGAEK
                     RVEWVNQLVSNSFNKKIRPLRTQSDWISSNPIEVDNFIKDPYSGFNVSNQLLYQTAYY
                     MLHTSQLKNMKMLNHAMPILLVSGYDDPLGDYGKGILKLANIYRKAGIKNVKVNLYHH
                     KRHEVLFEKDHDKIWEDLFKWLNQFYKK"
     gene            11015..11950
                     /gene="miaA"
                     /locus_tag="O0293_001293"
     CDS             11015..11950
                     /gene="miaA"
                     /locus_tag="O0293_001293"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCY0280182.1"
                     /translation="MNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYK
                     HMNIGTAKVTPEEMDGIPHHLIDILNPDDTFSAYEFKRLAEDLITDITNRGKVPIIAG
                     GTGLYIQSLIYNYELEDETVTPAQLSIVKQKLSALEHLDNQQLHDYLAQFDAVSAENI
                     HPNNRQRVLRAIEYYLKTKKLLSNRKKVQQFTENYDTLLLGIEMSRKTLYSRINKRVD
                     IMLDHGLFREVQQLVEQGYESCQSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYA
                     KRQMTWFKNKMSVHWLDKENMSLQMMLDEITTQIK"
     gene            11965..12198
                     /gene="hfq"
                     /locus_tag="O0293_001294"
     CDS             11965..12198
                     /gene="hfq"
                     /locus_tag="O0293_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCY0280183.1"
                     /translation="MIANENIQDKALENFKANQTEVTVFFLNGFQMKGVIEEYDKYVV
                     SLNSQGKQHLIYKHAISTYTVETEGQASTESEE"
     gene            complement(12420..12896)
                     /locus_tag="O0293_001295"
     CDS             complement(12420..12896)
                     /locus_tag="O0293_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCY0280184.1"
                     /translation="METIYDFVVETNKGVTYKLDAYKGDVMLIVNTASECGFTSQFEG
                     LQSLYEKYKDQGFVILGFPCNQFGGQEPGSGEEAAQNCKLNYGVTFPMHQKIDVKGEH
                     QLPLFRYLTAAQHGFFNEKIKWNFTKFLVDREGNVVKRFAPQKKPVQIEREIEKLL"
     gene            13008..>14018
                     /gene="hflX"
                     /locus_tag="O0293_001296"
     CDS             13008..>14018
                     /gene="hflX"
                     /locus_tag="O0293_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCY0280185.1"
                     /translation="MAQQQIHDTKNKLEKAVLVGVHAQDDKQFNFESTMEELSSLSET
                     CQLEVLGQITQNRDRVDRKYYVGKGKIEEIQAFIEFKDIDVVITNDELTTAQSKSLNE
                     ALGVKIIDRTQLILEIFALRARSKEGKLQVELAQLDYLLPRLQGHGKSLSRLGGGIGT
                     RGPGETKLEMDRRHIRTRMNEIKHQLRTVEEHRERYRNKRNQNQVFQVALVGYTNAGK
                     SSWFNVLANEETYEKDQLFATLDPKTRQIQINDGFNLIISDTVGFIQKLPTTLIAAFK
                     STLEEAKGADLLVHVVDSSHPEYRTQYDTVNDLIKQLDMSHISQIVIFNKKDLCDHAS
                     NRP"
BASE COUNT         5219 a         1910 c         2662 g         4227 t
ORIGIN      
        1 ataatatgtc taatgttaca ccaatgatgc agcaatattt aaaaataaaa tcagaatacc
       61 aagattgctt attatttttt agactaggtg atttctatga aatgttttat gaagatgcca
      121 aggaggcatc acgtgtactt gaaattactt taactaaaag agatgctaaa aaagaaaatc
      181 caattccgat gtgtggtgtt ccgtatcatt ctgcagatag ttatatagat acacttgtta
      241 ataatggata taaagtagct atttgtgaac agatggaaga tccgaaacaa acgaaaggta
      301 tggttagacg tgaggtagta agaattgtga ctccaggaac tgtgatggag caaggtggtg
      361 tagatgataa acaaaataac tatattttaa gttttgttat gaatcaacct gaaattgcgc
      421 ttagttactg tgatgtttct actggcgaat taaaggttac acattttaat gatgaagcga
      481 ctttattaaa tgaaattacg acgataaacc ctaacgaagt tgttatcaat gacaatattt
      541 ccgataattt aaaaagacaa attaatatgg tgacagaaac aataacagtc agggaaacgt
      601 tatcatcaga aatctatagt gtgaatcaaa ctgaacataa attaatgtat caagcgacac
      661 aattattgct agattatatt catcatacac aaaaacgtga tttatcgcat atcgaggatg
      721 ttgttcaata tgcagctata gattatatga aaatggattt ttatgctaag agaaaccttg
      781 agttaacgga aagcattcga ttaaaatcaa aaaaaggaac gctactttgg ctaatggacg
      841 aaacgaaaac accaatggga gcacgccgct taaaacaatg gatagataga ccactaataa
      901 gtaaagaaca aattgaagca cgattagata tcgttgatga atttagtgct catttcatag
      961 aaagagacac cttaagaaca tatcttaatc aagtgtatga tattgaacgt cttgttgggc
     1021 gtgttagtta cggaaatgtt aatgcgagag atttaattca acttaaacat tccatttctg
     1081 aaataccgaa tattaaagca ttactaaatt ctatgaatca gaatactctt gtacaagtta
     1141 atcaactaga accccttgat gatttacttg atatattaga acagagttta gtagaagaac
     1201 caccaatttc agttaaagat ggcggactat tcaaagttgg ttttaatacg caattagatg
     1261 aatatcttga agcttcaaaa aacggaaaaa catggttagc agaattacaa gccaaagaaa
     1321 gacaacgtac aggaataaaa tcattgaaaa taagctttaa taaagtgttt ggttatttta
     1381 tagaaataac acgtgccaac ttgcaaaatt ttgaaccaag tgaatttggt tatatgagga
     1441 agcaaacgtt atcgaatgct gaacgtttta taactgatga acttaaagaa aaagaagata
     1501 tcattttagg tgcggaagac aaagccatcg aattagaata tcaattattt gttcagctac
     1561 gtgaagaagt taaaaaatat actgaacgtt tacaacaaca agctaaaatt atttcagagc
     1621 tagattgttt acagagcttt gcagaaattg ctcaaaaata taattacact aggccttcat
     1681 ttagtgaaaa taaaacatta gaattagtgg aatctaggca cccagtagtg gaaagagtaa
     1741 tggattataa tgactatgtg cctaataatt gtcgattaga taatgaaaca tttatatatt
     1801 taattacagg tccgaatatg tctggtaaat cgacatatat gagacaagtt gccataatta
     1861 gtataatggc ccaaatggga gcttatgtcc cttgtaaaga ggcagtgtta cctatatttg
     1921 atcaaatatt cactagaata ggtgcggcag atgatttggt ttcaggtaag agtacgttta
     1981 tggtagaaat gctagaagca caaaaggcat taacttatgc aacagaggat agtttgatta
     2041 ttttcgatga aattggacgt ggtacttcaa cgtatgacgg tttagcttta gcgcaggcaa
     2101 tgatagagta tgtagctgaa acatcacatg ctaaaacgtt attttcaaca cattatcatg
     2161 aattgacaac attagatcaa gcattaccaa gtctaaaaaa tgttcacgtc gctgctaatg
     2221 aatataaagg tgaacttata ttcttgcata aagtcaaaga tggtgcagtt gacgatagtt
     2281 atggtattca agttgcgaaa ttagctgatt tacctgaaaa agttattagc agagcacaag
     2341 tgattctaag cgagtttgaa gcgtctgctg gtaaaaaatc atcgatatca aatttaaaaa
     2401 tggtcgaaaa tgaacctgaa attaatcaag aaaatttaaa cttaagtgtt gaagaaacaa
     2461 ctgatacttt atctcaaaaa gactttgaac aagcatcatt tgatttgttt gaaaatgatc
     2521 aagaaagcga gattgaacta caaattaaaa atttgaattt atctaatatg acaccaattg
     2581 aggcattggt gaagttaagt gaattacaaa atcaattaaa atagaggtgt tgcaaaatgg
     2641 ggaaaattaa agaactccaa acctcattag caaataaaat cgcagcaggt gaagtagttg
     2701 aaagaccgag ttctgttgtg aaagaactgt tggaaaatgc tatagatgca ggcgctacag
     2761 aaataagcat tgaagtagag gaatctggcg tccaatctat tcgcgtagtc gataatggaa
     2821 gcggaattga agcggaagac ttaggattag tatttcatag acatgcgact agtaaattag
     2881 atcaagatga agatttattt catattagga cattaggatt ccgtggtgaa gcactagcca
     2941 gtatttcatc agttgctaaa gtaacattga agacttgcac ggataatgct aatggaaatg
     3001 aaatatatgt agaaaatggt gaaatattaa atcataagcc tgcaaaagcg aaaaaaggaa
     3061 cagatatact tgtagaatca ttattttata atacaccagc acgtttaaaa tatattaaaa
     3121 gtttatacac tgaactaggt aaaataacag atattgtcaa cagaatggca atgagccatc
     3181 cggacattcg aatagcactc atttcagatg gcaaaacaat gttaagtaca aatggttcag
     3241 gacgaactaa tgaagtgatg gcagagattt atgggatgaa agttgcacga gatttagtac
     3301 atatatctgg agatacaagt gattatcaca ttgaaggttt tgttgcaaag cctgaacatt
     3361 ctagaagtaa taagcactat atttctattt ttattaatgg acgatacatt aaaaacttta
     3421 tgctaaataa agcgatttta gaaggctatc atacactctt aacaataggt aggttcccga
     3481 tttgttatat taatattgaa atggatccaa tcttagtaga cgtaaatgtt catccaacaa
     3541 aactagaagt gcgtttatca aaagaagagc aactatacca attgattgtg agcaaaatac
     3601 aagaagcatt taaagaccgt atattaattc ctaaaaataa cttggattat gtgccaaaaa
     3661 aaaataaagt attacattca ttcgaacaac aaaaaatcga atttgaacaa agacaaaaca
     3721 cagagaataa ccaagagaag acgttttcat ctgaagaaag taacagtaag ccatttatgg
     3781 aagaaaatca aaacgatgag atagttataa aagaagattc atataatcca ttcgtaacga
     3841 aaacatctga aagtttaata gctgatgatg aatcttctgg ttataataat acacgtgaaa
     3901 aagatgaaga ctacttcaaa aaacaacaag aaattttaca agaaatggat caaacatttg
     3961 attcaaatga tggtacaact gtgcaaaatt atgagaataa agcgtctgat gattattatg
     4021 atgtaaacga tattaaagga acaaaaagta aagaccctaa acgaagaatt ccatatatgg
     4081 aaattgttgg acaagtacat ggaacgtata ttattgctca aaatgaattt ggcatgtaca
     4141 tgattgacca gcatgcagct caagaaagaa taaaatatga atattttcga gataaaatag
     4201 gtgaggttac caatgaagta caagatttat taatcccgtt aacatttcat ttttcaaaag
     4261 atgaacaatt agtcattgat caatataaaa atgagcttca acaagtaggt atcatgttag
     4321 aacattttgg tggtcatgat tatattgtaa gtagctatcc agtttggttc cctaaagatg
     4381 aagtagaaga aattattaaa gatatgattg agctaatttt ggaagagaaa aaagtagata
     4441 tcaaaaaatt acgtgaagat gtagcaatca tgatgtcatg taaaaaatcc attaaagcga
     4501 atcattattt acaaaaacat gaaatgtctg atttaattga tcaattaaga gaagcggaag
     4561 atccatttac atgtccacat ggtcgtccaa taatcattaa tttttcaaaa tacgaattag
     4621 aaaaattatt taagcgtgtg atgtagagag gatgaatcaa gtgaataaca acatattgcc
     4681 tgctataaga aacattaaag atttagagaa actgattaaa acagactata aaatgtgtgt
     4741 gcttctagat atgcatatag gacatataaa aagtattatg gaattgctga agcaaaatca
     4801 tatagagtgt tttattcata tagatttgat aaaaggttta agccacgatg aatttgcaag
     4861 tgaatttatt attcagcaat acaagccaaa aggtatcgta tcgactaaat ctaaagtaat
     4921 aaaaaaagct aaatcattaa atactttaac gatttttaga gtatttatta ttgatagtca
     4981 agcattgaaa cgcagtatag atttgataaa aaaagttgaa cctgattttg ttgaagtact
     5041 tccaggtgtt gcgagtaaag cgattcatca tattcagaaa gaaacaaaca cacaagtcat
     5101 tgcaggtggc ctaattaata caatagatga agtcaatgaa gctgttaaaa atggagcgaa
     5161 atatgtaact actagttatg ataaactttg gtaaatcata agtctgcaaa aatttcattt
     5221 ttcacgagat ttagtcatat aacataataa aaaagccgtt ttcatatggg atatgcagta
     5281 ggtatcaaac aaaaaataat caaataaaat aattagcacc tctaaaacga ttaaaatcat
     5341 taaagtttta gaggtgttta ttagtaaaaa atgcaactag tcataaaaaa tatggctgaa
     5401 gaatgtcaat taaacaaaag agcgtcataa ataaattagt taggattggt atttctagtt
     5461 ttcaaattaa actactttta taaagacttg aaaaattaaa tttgacaaaa tttttacaaa
     5521 aaccattgac aacgctttca tatcgatagt atcataacaa atataataaa agttaataca
     5581 tagaatagag acgggagatt tctacgagcc aaactgctag tgtaggaatc tctttgtctt
     5641 tttgggagga catttaatat gaatgtatat ttagcagaat tcctaggaac tgcaatctta
     5701 atcctttttg gtggtggcgt ttgtgccaat gtcaatttaa agagaagtgc tgcgaatggt
     5761 gctgattgga ttgtcatcac agctggatgg ggattagcgg ttacaatggg tgtgtttgct
     5821 gtcggtcaat tctcaggtgc acatttaaac ccagcggtgt ctttagctct tgcattagac
     5881 ggaagttttg attggtcatt agttcctggt tatattgttg ctcaaatgtt aggtgcaatt
     5941 gtcggagcaa caattgtatg gttaatgtac ttgccacatt ggaaagcgac agaagaagct
     6001 ggcgcgaaat taggtgtttt ctctacagca ccggctatta agaattactt tgccaacttt
     6061 ttaagtgaga ttatcggaac aatggcatta actttaggta ttttatttat cggtgtaaac
     6121 aaaattgccg atggtttaaa tcctttaatt gtcggagcat taattgttgc aatcggatta
     6181 agtttaggcg gtgctactgg ttatgcaatc aacccagcac gtgatttagg tccgagaatt
     6241 gcacatgcga ttttaccaat agctggtaaa ggtggttcaa attggtcata tgcaatcgtt
     6301 cctatcttag gaccaattgc cggtggttta ttaggtgcag tggtatacgc tgtattttat
     6361 aaacatacat ttaatattgg ttgtgcaatt gcaattgttg tagttattat tactttgatt
     6421 ttaggttaca ttttaaataa atcatcaaaa aaaggtgata tcgaatcaat ttactaaaat
     6481 aaaaagaaac gtaaatagca taatttaaca tgtttgattc atggattatg ctattttttc
     6541 gccaaaattt aacagatttt gtacaatggg ttagcgatta ttttttaata aaggagatac
     6601 tactaatgga aaaatatatt ttatctatag accaaggaac aacaagctca agagcgattt
     6661 tattcaatca aaaaggggaa attgcagggg tagcacaacg tgagtttaag caatattttc
     6721 cacaatcagg ttgggttgaa catgatgcaa atgaaatttg gacatctgtg ttagctgtaa
     6781 tgacggaagt aattaatgaa aatgatgtta gagctgatca aattgcaggt atcggtatta
     6841 caaaccaacg tgaaacaacg gttgtttggg acaaacatac tggccgccca atttatcacg
     6901 caattgtttg gcaatcacgt caaacacaat caatttgttc agaattaaaa caacaaggat
     6961 atgaacaaac atttagagat aagacaggat tacttttaga tccgtatttt gcaggtacaa
     7021 aagttaaatg gattctagac aatgttgaag gtgcacgaga aaaagcagaa aatggcgatc
     7081 tattatttgg aacgattgat acttggttag tatggaaatt atcaggaaaa gctgcgcata
     7141 ttactgatta ttcaaatgcg agtcgtacat taatgtttaa tatccatgat ttagaatggg
     7201 acgatgagtt attagaacta cttacagtac ctaaaaatat gttgccagaa gttaaagctt
     7261 cgagtgaagt atatggtaag acaattgatt accacttcta tggtcaagaa gtaccaatcg
     7321 ctggagtagc tggtgatcaa caagcagcat tatttggaca agcttgcttc gaacgtggtg
     7381 acgtgaaaaa cacatatgga actggtggct tcatgttaat gaatacaggt gacaaagcgg
     7441 ttaaatctga aagtggttta ttaacaacaa ttgcttatgg tattgatgga aaagtaaatt
     7501 atgcgcttga aggttccatc tttgtttcgg gttcagcaat ccaatggtta cgtgatggat
     7561 taagaatgat taattcagca ccacaatcag aaagttatgc gacacgagtt gactctactg
     7621 agggtgttta tgttgttcca gcttttgtag gtttaggaac accatattgg gattctgaag
     7681 cacgtggtgc gattttcggt ttaacacgtg gaactgaaaa agagcacttt atccgtgcaa
     7741 ctttagaatc actatgttac caaactcgtg acgttatgga agcaatgtca aaagactctg
     7801 gtattgatgt ccaaagttta cgtgtcgatg gtggtgcagt taaaaataac tttattatgc
     7861 agttccaagc agacattgtt aatacttctg ttgaaagacc tgaaattcaa gaaactacag
     7921 ctttaggtgc tgcattttta gcaggtttag cagttggatt ctgggagagt aaagatgata
     7981 tcgctaaaaa ctggaaatta gaagaaaaat tcgatccgaa aatggatgaa ggcgaaagag
     8041 aaaaattata tagaggttgg aaaaaagctg ttgaagcaac acaagttttt aaaacagaat
     8101 aaacttgtag attagacttt tgtataaaca ttgtgataca atcaatttaa gttaatattt
     8161 gaatcgagaa gcgagagatt tgttcgaaca tgtacaattg aaggattgtt tatgtgggac
     8221 aagtctctcg tttttacatt ttttaggagg cgttttggaa tggcattgtc tacttttaag
     8281 agagaacata ttaaaaagaa tttaagaaat gatgaatatg atttagtaat tattggtggc
     8341 ggtattacag gtgcaggtat tgcactagac gcgagtgaaa gaggaatgaa agttgcatta
     8401 gttgaaatgc aagactttgc acaaggaaca agctcaagat ctacaaaatt agtccatggt
     8461 ggtttgcgtt acttaaaaca attccaaatt ggagtagttg ccgaaactgg taaagaacgt
     8521 gcgattgttt atgaaaatgg gcctcatgtt acgactccag agtggatgct tttaccaatg
     8581 cataaaggtg gaacatttgg taaattctca acatcaattg gtttaggaat gtatgatcgt
     8641 ttagcaggtg ttaagaagtc tgaacgtaaa aaaatgttat ctaaaaaaga aactttagct
     8701 aaagaaccat tagttaaaaa agaaggtcta aaaggcggcg gttactatgt tgaatatcgt
     8761 actgacgatg cgcgtttaac tattgaagtt atgaagcgtg ctgctgaaaa aggcgcagaa
     8821 attatcaact atactaaatc tgaacacttc acttatgata aaaatcaaca agtaaatggt
     8881 gttaaagtta tagataaatt aactaatgaa aattatacaa ttaaggctaa aaaagtggtt
     8941 aatgcagcag gtccatgggt tgatgatgtt agaagtggtg attatgcacg caataataaa
     9001 aaattacgtt taactaaagg tgtacatgtt gttattgatc aatcaaaatt cccattaggt
     9061 caagcagtat actttgatac tgaaaaagat ggaagaatga tttttgcaat tccacgtgaa
     9121 ggaaaagcgt atgtaggtac tacagataca ttctatgaca atatcaaatc ttcaccatta
     9181 actacacaag aagacagaga ctatttaatc gatgcgatta attacatgtt ccctagtgtt
     9241 aatgttacag atgaagatat tgaatcaaca tgggcaggaa ttagaccatt aatttacgaa
     9301 gaaggcaaag acccttctga aatctctcgt aaggatgaaa tttgggaagg taaatcaggt
     9361 ttattaacta ttgcaggtgg taaattaaca ggctatcgtc acatggctca agacattgtt
     9421 gatttagtat ctaaacgctt gaaaaaagac tacggtttaa catttagtcc atgtaataca
     9481 aaaggtctgg caatttcagg tggcgatgta ggtggtagca agaactttga tgcgtttgta
     9541 gagcaaaaag tagatgtagc taaaggattc ggcattgatg aagatgttgc aagacgttta
     9601 gcatctaaat atggttcaaa tgttgatgaa ttgttcaaca ttgcgcaaac atctcaatac
     9661 catgatagca agttaccatt agaaatttat gtagaacttg tttatagtat tcaacaagaa
     9721 atggtataca aacctaacga tttcttagtt cgtcgttctg gtaaaatgta tttcaatatt
     9781 aaagatgtat tagattataa agatgctgtc atcgatatta tggcagatat gcttgattac
     9841 tctccagctc aaattgaagc atatactgaa gaagttgagc aagcaattaa agaagcgcaa
     9901 catggaaata atcaaccagc agttaaagaa taattaattt gtacaatcat aaactggtgt
     9961 cctgttttaa gggcatcagt ttttttatac gagatacatt agtcaattga aactatgcat
    10021 cactaaatgt atgataataa taaaagcgaa tataaaagcg ttgttattaa aggtgtgaga
    10081 tgatgactga aaaacaattt aaattaactg tacaagataa tacgaatatt gaagttaaag
    10141 tgaattttac agatgtagat tcaaaaggaa ttattcatat atttcatggt atggctgaac
    10201 atatggaacg ttacgataaa ttagcacatg cactttcaaa gcatggcttc gatgtgatac
    10261 gtcataatca tcgaggacat ggtattaata ttgatgaatc aacaagaggg cattacgatg
    10321 atatgaaacg agttatcggt gatgcctttg aagtagcgca aacagtgaga ggcaatgttg
    10381 ataaaccata cattataatc ggacattcaa tgggatccgt tatagctaga ttgtttgtag
    10441 aaacatatcc gcaatatgtt gatggtctaa ttttaagtgg tactggtatg tattcattat
    10501 ggaaaggttt accaaccgtt aaagtgttac aactgattac aaaaatttat ggtgctgaga
    10561 aacgagttga atgggttaac cagttagtat caaatagttt taataaaaaa atacgtccat
    10621 tacgtacaca aagtgattgg atttctagta atccaattga agtagataac tttattaaag
    10681 atccatatag tggatttaat gtgtcaaatc aattattata tcaaacagcc tattatatgc
    10741 tacatacatc acaattaaaa aatatgaaaa tgttaaatca tgccatgcct atattattag
    10801 tttcaggata tgacgatcct ttaggtgatt atggtaaagg gattttaaaa ttggcgaata
    10861 tatatagaaa agctggcatt aaaaatgtta aagtgaatct ttatcatcat aaacgtcatg
    10921 aagtgttatt tgaaaaagat catgacaaaa tttgggaaga cttgtttaaa tggttgaatc
    10981 aattttataa aaaataaaga aagtggaatt aaatatgaat aaaaataagc cttttattgt
    11041 agtaattgtg gggccaactg cttcaggtaa aacagagctt agcatcgaac tcgcgaagcg
    11101 tatcaatggt gaaatcataa gcggtgactc tatgcaagtc tacaaacata tgaatattgg
    11161 aactgcaaaa gtaacacctg aagaaatgga tggtattcca catcatttaa ttgatatctt
    11221 gaatcctgat gatacatttt cagcatatga attcaagcga ttagcagaag atttaattac
    11281 tgatataacg aatagaggta aagttccaat catagcaggt ggaacaggct tatatattca
    11341 atcattaata tataattatg aattagaaga tgaaacagtt acacctgcac aattatccat
    11401 agttaaacaa aagttatctg cattagaaca tttagataat cagcaactac acgattattt
    11461 agctcaattt gatgcggttt ctgcagaaaa tattcaccct aacaaccgcc aaagagtgtt
    11521 gcgcgctatt gaatattatt taaaaacaaa aaaacttttg agtaatcgca agaaagtgca
    11581 acaatttact gaaaattatg atacattatt attagggatt gaaatgtcgc gtaaaacatt
    11641 atattcaaga ataaataaac gtgttgatat tatgttggat cacggattat ttagagaagt
    11701 gcaacaactt gttgaacaag gctatgaatc ttgccaaagt atgcaagcta ttggatataa
    11761 agaattaata cctgtgatta acggacaaat gatttatgaa gatgctgtca atgatttaaa
    11821 gcaacattca cgccaatatg caaaacgaca aatgacatgg ttcaagaata aaatgagtgt
    11881 tcattggtta gataaagaaa atatgtcact tcaaatgatg ttagatgaga ttacaaccca
    11941 gattaagtaa aaggagtccg acagatgatt gcaaacgaaa acatccaaga caaagcacta
    12001 gagaatttta aagcaaacca aactgaagta actgtattct ttctaaacgg tttccaaatg
    12061 aaaggtgtta ttgaagaata cgacaagtat gtcgtaagct taaattctca aggcaaacaa
    12121 cacttgattt acaaacatgc gatcagcact tatacagtag aaactgaagg tcaagcatct
    12181 actgaaagtg aagaataagt tgtaatcata tgggcacgat ttaatgactc gtgtacataa
    12241 cgtttgaagt atgataaata gatatagaaa taaagtcata tgtgacatca attaaatgat
    12301 gttcaaatga caagatacaa tagaggaatg tttgtaataa aaacgcttca tataaaggtc
    12361 gagtcaatat atgatacgac tttatatgaa gcgttttatt tgctatgagc tagtatattt
    12421 tataataatt tttctatttc tctttcgatt tgaacaggtt ttttttgagg tgcaaatcgt
    12481 ttaacaacgt taccttcgcg atccactaaa aacttagtga aattccattt gattttctca
    12541 ttaaagaatc cgtgttgtgc cgcagtcaaa tatctaaata aaggtaattg atgttcccct
    12601 tttacgtcta ttttttgatg catagggaag gtaacaccat agtttaattt acagttttga
    12661 gctgcttctt cgcctgaacc aggttcttgg ccaccaaatt gattacaagg gaaacctaga
    12721 attacaaacc cttgatcttt gtatttctcg tataatgatt gcaaaccttc aaattgtgaa
    12781 gtaaagccac attcgctagc tgtattaaca attagcataa cgtcaccctt atatgcatct
    12841 aatttgtaag taacaccttt atttgtttct actacaaaat cataaattgt ctccattgta
    12901 tcatcctttc gatttactta aaatgtacca caaaatcgtg taatagtctt tactaaaact
    12961 ctatgataga atactttgag taggatttta ttaaggagat gtataacatg gctcagcaac
    13021 aaattcatga tactaaaaat aaactagaaa aagctgtctt agtcggtgta catgctcaag
    13081 atgataagca atttaatttt gagtctacaa tggaggaatt atcatcttta tcagagactt
    13141 gccaacttga agtgttgggt caaattactc aaaacagaga tcgtgtagat cgcaaatatt
    13201 atgttggtaa aggtaaaatt gaagaaattc aagcatttat tgagttcaaa gatattgatg
    13261 tagtcatcac aaatgatgaa ttaacgactg cacaatccaa atcactaaat gaagctttag
    13321 gtgtaaaaat tattgataga actcagttga ttcttgaaat atttgcatta agagcaagaa
    13381 gtaaagaagg taaattgcaa gtagagctag cacaacttga ttatttatta cctagattgc
    13441 aaggccatgg taaaagcctt tctcgtttag gtggcggtat tggaactaga ggccctggtg
    13501 aaacgaagtt agagatggat cgcagacata ttcgaactcg tatgaatgaa attaaacatc
    13561 aattgcggac ggtagaagaa catcgcgaaa gatatcgaaa taaaagaaat caaaatcagg
    13621 tgtttcaagt agctttagtt ggttatacaa atgctggtaa atcatcatgg tttaatgttt
    13681 tagcaaatga agagacgtat gaaaaagatc aattatttgc aacgttagat cctaaaacac
    13741 gacaaattca aataaatgat ggatttaatt taattatttc agatactgtt ggttttatac
    13801 agaaactacc tacgacgtta attgcagctt ttaaatcaac tttagaagag gctaaaggtg
    13861 cagatttatt agtacatgtc gtagatagta gccatcctga ataccgtacg cagtatgaca
    13921 cagttaatga tttaatcaaa caattagata tgagtcatat ttctcaaata gttattttta
    13981 ataaaaagga cttatgtgat catgcatcaa atcgtcca
//
LOCUS       DAKTIO010000057        13812 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.057, whole genome shotgun sequence.
ACCESSION   DAKTIO010000057 DAKTIO010000000
VERSION     DAKTIO010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13812
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.057"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            29..2734
                     /gene="acnA"
                     /locus_tag="O0293_001297"
     CDS             29..2734
                     /gene="acnA"
                     /locus_tag="O0293_001297"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCY0280186.1"
                     /translation="MAANFKEQSKKHFDLNGQSYTYYDLKAVEEQGITKVSNLPYSIR
                     VLLESLLRQEDDFVITDDHIKALSQFGKDGNEGEVPFKPSRVILQDFTGVPAVVDLAS
                     LRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFL
                     NWATKAFDNYNAVPPATGIVHQVNLEYLASVVHVRDVDGEKTAFPDTLVGTDSHTTMI
                     NGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLVNSLPQGATATDLALRVTQELR
                     KKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDDESLKYMKLTGRSD
                     EHIALVKEYLKQNHMFFDVEKEDPNYTDVIELDLSTVEASLSGPKRPQDLIFLSDMKS
                     SFENSVTAPAGNQGHGLDKSEFDKKAEINFKDGSKATMKTGDIAIAAITSCTNTSNPY
                     VMLGAGLVAKKAVEKGLKVPEYVKTSLAPGSKVVTGYLRDAGLQPYLDDLGFNLVGYG
                     CTTCIGNSGPLLPEIEKAIADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAY
                     ALAGTVDIDLQNEPIGKGNDGEDVYLKDIWPSIKEVSDTVDSVVTPELFIEEYNNVYN
                     NNELWNEIDVTDQPLYDFDPNSTYIQNPSFFQGLSKEPGTIVPLNGLRVMGKFGDSVT
                     TDHISPAGAIGKDTPAGKYLQDHQVPIREFNSYGSRRGNHEVMVRGTFANIRIKNQLA
                     PGTEGGFTTYWPTNEVMPIFDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLG
                     VKTVIAQSYERIHRSNLVMMGVLPLEFKKGESADSLGLDGTEEISVNIDENVQPHDYV
                     KVTAKKQDGDLVEFDAMVRFDSLVEMDYYRHGGILQMVLRNKLAQ"
     gene            2914..3381
                     /locus_tag="O0293_001298"
     CDS             2914..3381
                     /locus_tag="O0293_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCY0280187.1"
                     /translation="MIYSITEIEARYAETDKMGVIYHGNYATWFEVARLDYISKLGFS
                     YADMEKQGIISPVTDLNVNYKKSIFYPEKVKVKTWVEKYSRLRSVYKYEIFNEKGELA
                     TTGSTELICIKEDTFKPIRLDRYFPDWHEAYSKVQTLNNEGKTVEIMDGIDSL"
     gene            complement(3588..3884)
                     /locus_tag="O0293_001299"
     CDS             complement(3588..3884)
                     /locus_tag="O0293_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HCY0280188.1"
                     /translation="MQIELTDAAVTWFKNELELPENNKVLVFFVRYGGEFQLKQGFSP
                     AFTVEPKEDVDIGYEQQYDDLNVVVAEKDLWYFEDDHIIVNVVDHEDEISYSTK"
     gene            complement(4260..4868)
                     /gene="plsY"
                     /locus_tag="O0293_001300"
     CDS             complement(4260..4868)
                     /gene="plsY"
                     /locus_tag="O0293_001300"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCY0280189.1"
                     /translation="MMIIVMLLLSYLIGAFPSGFVIGKLFFKKDIRQFGSGNTGATNS
                     FRVLGRPAGFLVTFLDIFKGFITVFFPLWLQVHADGPISTFFTNGLIVGLFAILGHVY
                     PVYLKFQGGKAVATSAGVVLGVNPILLLILAIIFFIVLKIFKYVSLASIVAAICCVIG
                     SLIIQDYILLVVSFLVSIILIIRHRSNIARIFRGEEPKIKWM"
     gene            5074..7065
                     /gene="parE"
                     /locus_tag="O0293_001301"
     CDS             5074..7065
                     /gene="parE"
                     /locus_tag="O0293_001301"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCY0280190.1"
                     /translation="MNKQNNYSDDSIQVLEGLEAVRKRPGMYIGSTDKRGLHHLVYEI
                     VDNSVDEVLNGYGNEIDVTINKDGSISIEDNGRGMPTGIHKSGKPTVEVIFTVLHAGG
                     KFGQGGYKTSGGLHGVGASVVNALSEWLEVEIHRDGNIYHQSFKNGGSPSSGLVKKGK
                     TKKTGTKVTFKPDDTIFKASTSFNFDVLSERLQESAFLLKNLKITLNDLRSGKERQEH
                     YHYEEGIKEFVSYVNEGKEVLHDVATFSGEANGIEVDVAFQYNDQYSESILSFVNNVR
                     TKDGGTHEVGFKTAMTRVFNDYARRINELKTKDKNLDGNDIREGLTAVVSVRIPEELL
                     QFEGQTKSKLGTSEARSAVDSVVADKLPFYLEEKGQLSKSLVKKAIKAQQAREAARKA
                     REDARSGKKNKRKDTLLSGKLTPAQSKNTEKNELYLVEGDSAGGSAKLGRDRKFQAIL
                     PLRGKVINTEKARLEDIFKNEEINTIIHTIGAGVGTDFKIEDSNYNRVIIMTDADTDG
                     AHIQVLLLTFFFKYMKPLVQAGRVFIALPPLYKLEKGKGKTKRVEYAWTDEELNKLQK
                     ELGKGFTLQRYKGLGEMNPEQLWETTMNPETRTLIRVQVEDEVRSSKRVTTLMGDKVQ
                     PRREWIEKHVEFGMQEDQSILDNSEVQVLENDQFDEEEI"
     gene            7065..9467
                     /gene="parC"
                     /locus_tag="O0293_001302"
     CDS             7065..9467
                     /gene="parC"
                     /locus_tag="O0293_001302"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCY0280191.1"
                     /translation="MSEIIQDLSLEDVLGDRFGRYSKYIIQERALPDVRDGLKPVQRR
                     ILYAMYSSGNTHDKNFRKSAKTVGDVIGQYHPHGDYSVYEAMVRLSQDWKLRHVLIEM
                     HGNNGSIDNDPPAAMRYTEAKLSLLAEELLRDINKETVSFIPNYDDTTLEPMVLPSRF
                     PNLLVNGSTGISAGYATDIPPHNLAEVIQATLKYIDNPDITVNQLMKYIKGPDFPTGG
                     IIQGIDGIKKAYESGKGRIIVRSKVEEETLRNGRKQLIITEIPYEVNKSSLVKRIDEL
                     RADKKVDGIVEVRDETDRTGLRIAIELKKDVNSESIKNYLYKNSDLQISYNFNMVAIS
                     DGRPKLMGIRQIIDSYLNHQIEVVANRTKFELDNAEKRMHIVEGLIKALSILDKVIEL
                     IRSSKNKRDAKENLIEVYEFTEEQAEAIVMLQLYRLTNTDIVALEGEHKELEALIKQL
                     RHILDNHDALLNVIKEELNEIKKKFKSERLSLIEAEIEEIKIDKEVMVPSEEVILSMT
                     RHGYIKRTSIRSFNASGVEDIGLKDGDSLLKHQEVNTQDTVLVFTNKGRYLFIPVHKL
                     ADIRWKELGQHVSQIVPIEEDEVVINVFNEKDFNTDAFYVFATQNGMIKKSTVPLFKT
                     TRFNKPLIATKVKENDDLISVMRFEKDQLITVITNKGMSLTYNTSELSDTGLRAAGVK
                     SINLKAEDFVVMTEGVSENDTILMATQRGSLKRISFKILQVAKRAQRGITLLKELKKN
                     PHRIVAAHVVTGEHSQYTLYSKSNEEHGLINDIHKSEQYTNGSFIVDTDDFGEVIDMY
                     IS"
     gene            9717..11177
                     /locus_tag="O0293_001303"
     CDS             9717..11177
                     /locus_tag="O0293_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCY0280192.1"
                     /translation="MKDFDSLIPGWFKEFVHVGTDLIWSQYLIGLLLTAGFFFTISSK
                     FVQLRMLPEMFRALVERPETLEDGKKGISPFQAFAISAGSRVGTGNIAGVATAIVLGG
                     PGAVFWMWVIAFIGAASAFIEATLAQVYKVHDKDGGFRGGPAYYITKGLNQKWLGIVF
                     AILITITFAFVFNTVQSNTIAESLNTQYNISPVITGIILAIVTAIIIFGGVRSIATLS
                     SLIVPIMAIIYIGMVLVILLFNLDQIVPMIGTIIKSAFGIEQVTGGAVGAAVLQGIKR
                     GLFSNEAGMGSAPNAAATAAVPHPVKQGLIQSLGVFFDTMLVCTATAIMILLYSGLKF
                     GDNAPQGVAVTQSALNEHLGSAGGIFLTIAVTLFAFSSVVGNYYYGQSNIEFLSTNRV
                     ILFIFRCLVVVLVFVGAVVKTETVWNTADLFMGLMAIVNIISIIGLSNVAFALMKDYQ
                     KQKKEGKNPVFKPENLEINLFGISAWGANKYKNSDK"
     gene            11677..12528
                     /locus_tag="O0293_001304"
     CDS             11677..12528
                     /locus_tag="O0293_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCY0280193.1"
                     /translation="MGEYIVTKTLNNNVVVCTNNDQEVILIGKGIGFNKKEGMALNDQ
                     TITIEKIYKLESEQQKAHYKSLVEIADDNVLQVIIDSLNFISNTAMNVDSKQLVVSLT
                     DHIIFAYKRLKQNQVISNPFVMETMQLYSDAYHIAKQVIDQLNAALDVHFPEDEIGFI
                     ALHIASNTEDLSMHEMTLINNVIKKGIDIIESDLVTTVDKESLQYQRFIRHVQFLIRR
                     LRRKEYIHAQDDFVSMIKNHYPICYNTAYKILTMIQKQFDVNISESEIIYLTLHIHHF
                     EERINQS"
     gene            12503..12670
                     /locus_tag="O0293_001305"
     CDS             12503..12670
                     /locus_tag="O0293_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280194.1"
                     /translation="MKKGLINPKLSVLHGRFVTVKGICMVSLMLVILHKWTVFIQHLM
                     NILSGGESIIE"
     gene            12663..>13812
                     /locus_tag="O0293_001306"
     CDS             12663..>13812
                     /locus_tag="O0293_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCY0280195.1"
                     /translation="MNENEKNIRKNFLKFTESRYMKFVGGNDLVFSLIALVLLGIVIF
                     IFEKVSYVFDPFIIVFKTIAAPIIVSLILFYLFNPIVNMMERYRIPRVAGISIIYLAV
                     VGVITLIVNLLIPIIGSQVDSLVKNSPQYLEKLINSIDKIANNTFFSSYYSQINDWLN
                     SLPKKIPSMLSEFTDGFGSKIATFAETIANIGVVIVTTPFVLFFMLKDGHHFKEFSTN
                     IMPPKFRKDFHDLLEKMSVQVGSYIQGQIIVSFCIGILLFIGYSVIGLKYSLVLASIA
                     AVTSVVPYLGPTIAISPAIVIAAITSPWMLLKLAVVWTLVQFVEGHFISPNIMGKTLK
                     IHPLTIIFILLCAGKLLGIVGVILGIPGYAILKVLVTHLFQLFKRRYNR"
BASE COUNT         4807 a         1944 c         2588 g         4473 t
ORIGIN      
        1 ataaaaatgt atcaaggggg atcattaaat ggctgcaaat tttaaagagc aatcaaaaaa
       61 acattttgac ttgaatggcc aaagttatac ttactatgat ttaaaagctg tagaagagca
      121 aggtattact aaagtttcca atttacctta ttcaattcgt gttttgttag aatctttact
      181 tcgtcaagaa gatgattttg taattacaga cgatcatatt aaagctttaa gtcagtttgg
      241 aaaagatgga aatgaaggcg aggtgccatt taaaccttct cgtgttattt tacaagattt
      301 cacaggtgta ccagccgtag ttgatttagc ttctttacgt aaagcaatgg atgacgttgg
      361 gggagatatt actaaaatta atccagaagt accggtggat ttagttattg accactcagt
      421 tcaagtggat agctatgcaa atccagaagc tcttgaacgt aatatgaaat tagaatttga
      481 acgtaactat gaacgttatc agtttttaaa ttgggcaacg aaagcatttg ataattacaa
      541 tgcagttcct cctgcaactg gaatagttca ccaagttaac ttagaatatt tagcaagtgt
      601 tgtacatgtt cgtgatgtag atggtgaaaa aactgcattt ccagatacat tagttggtac
      661 tgattcacat acaacaatga taaatggtat tggcgtacta ggatggggtg ttggtggtat
      721 tgaagctgaa gctggaatgc ttggacaacc ttcttatttc ccaattccag aggttattgg
      781 tgtacgacta gtaaattcat taccacaagg cgcaacagca actgatttag cgttaagagt
      841 aactcaagag ctacgtaaaa aaggtgttgt tggtaaattt gtggagttct ttggtccagg
      901 tgtacaacat ttaccactag cagaccgtgc tacaattgca aacatggcac cagagtatgg
      961 agcaacttgc ggattcttcc cagttgatga tgaatctctt aaatatatga agttaactgg
     1021 tagatcagac gaacatatcg cgctagtaaa agaatatttg aaacaaaacc atatgttctt
     1081 tgatgttgag aaagaagatc ctaattatac agatgttatc gaattggatt tatcaacagt
     1141 tgaagcatcg ctttcaggac caaaacgtcc tcaagattta attttcttaa gtgatatgaa
     1201 atcatcattt gaaaattctg taacagctcc agcaggcaac caaggacacg gtttagataa
     1261 aagtgaattt gataagaaag ctgaaattaa ctttaaagat ggatcaaaag ctacaatgaa
     1321 aacaggtgat attgcaatag cagcaattac atcatgtaca aatacatcta acccttatgt
     1381 aatgttaggt gcaggtttag ttgctaaaaa agcagttgaa aaaggcttga aagttcctga
     1441 atacgttaaa acttctctag caccaggatc aaaagttgtt accggatatt taagagatgc
     1501 tggcttacaa ccttatttag atgatttagg cttcaacttg gttggttatg gatgtacaac
     1561 ttgtatcggt aattcaggtc ctttattacc agaaattgaa aaagcgattg ctgatgagga
     1621 cctattagtg acatctgtat tatctggtaa ccgtaacttt gaaggtcgta tccatcctct
     1681 tgttaaagcc aattacctag cttcaccaca gttagttgtt gcttatgcat tagctggaac
     1741 ggttgatatt gatttacaaa atgaacctat tggtaaaggt aatgacggtg aagatgtata
     1801 tttgaaagat atttggccat caattaaaga agtttcagat accgttgata gtgttgtaac
     1861 acctgaatta tttattgaag aatataataa cgtatacaat aacaacgaat tatggaatga
     1921 gattgatgta actgatcaac ctctatatga ctttgatcct aattcaacat acattcaaaa
     1981 tccatcattc ttccaaggat tatctaaaga accgggtacg attgttccat taaatggttt
     2041 acgtgttatg ggtaaattcg gtgattctgt gacaactgac cacatctctc cagcaggtgc
     2101 aattggtaaa gatacgccag ctggtaaata tttacaagat catcaagtgc ctattcgtga
     2161 atttaattca tatggttcaa gacgtggtaa tcacgaagta atggttcgag gtacgtttgc
     2221 taatatacgt attaaaaacc aattagcgcc aggtactgaa ggtggtttta caacttattg
     2281 gccaacaaat gaagtaatgc ctatctttga tgctgcaatg aaatataaag aagatggtac
     2341 aggtttagtt gtattagctg gtaacgatta tggtatgggt tcatctcgtg actgggcagc
     2401 aaaaggtaca aacttattag gtgttaaaac agttattgca caaagttatg aacgtatcca
     2461 tcgttcaaat ttagttatga tgggtgtatt accattagag tttaaaaaag gtgaatcagc
     2521 tgattctctt ggtctagatg gtacagaaga aatttctgtt aatattgatg aaaatgttca
     2581 accacatgac tacgtcaaag ttactgctaa gaagcaagat ggtgatttgg tagaatttga
     2641 cgctatggtt cgttttgact cacttgttga aatggattac tatcgtcacg gtggaatttt
     2701 acaaatggtt ttaagaaata aattagcgca ataaaaaata gatatcacag taaaatttta
     2761 atcggtattt gaggcacttt gatagtgctc aaaaatactt ctataaactt tatgatataa
     2821 taggtcggtt caaaaccttt gtgttcttgg ccggcctctt cttgtatttt atgttagaat
     2881 gagatataaa gttaaactga aagaaggctt aaaatgatat atagtattac agaaatagaa
     2941 gcgcgttatg ctgaaactga taagatgggt gtaatttatc acgggaatta tgcaacttgg
     3001 tttgaagttg cgcggttgga ttatatatcg aagttaggtt ttagttatgc tgatatggaa
     3061 aaacaaggaa tcatttcacc tgtgactgac ctcaatgtca attataaaaa gtctattttt
     3121 tatccagaaa aagttaaagt taaaacttgg gttgaaaaat attcgagatt acgttcagtg
     3181 tataaatatg agatttttaa tgaaaaaggt gaacttgcaa ctacaggttc cacagaattg
     3241 atttgcatta aagaagatac ttttaagcct atacggttgg atcgttattt cccagattgg
     3301 catgaagctt atagtaaagt gcaaacgctc aataatgaag ggaaaacagt agagataatg
     3361 gatggtattg attctttata agttactgta aatgtttgga gaggcaatat taacaattaa
     3421 tctataaaaa atagccaata cgttaacatt aatgtaatta gcgtgaagat aagctaatat
     3481 atagcaaaaa cgaacttgtt catacaacat cagatgtgct aaaccaaagt acaacttccg
     3541 aggttcagga cacatcagaa tgtgtaaaca agttcgtttt attttagtta ttttgtggaa
     3601 taagaaattt catcttcgtg atcaactaca tttacaataa tgtggtcatc ttcaaagtac
     3661 cacaaatctt tttccgctac gacaacattt aaatcgtcat attgttgttc atagccaata
     3721 tcaacatctt cctttggttc aactgtaaaa gcaggactaa atccttgctt gagttggaat
     3781 tcgccaccat atcttacaaa aaacacgagc actttattat tttcaggcaa ctcaagttca
     3841 tttttaaacc aagttactgc tgcatcagta agttctattt gcataacaat gacctcctat
     3901 atttattaca gaatgggagt gggccaactt gtattatttg tggaatttct tatcgaaatt
     3961 ctctgtgttg gagcccaccc caacctgctc attattgtaa gctgaatttc cataagcttc
     4021 tatgttggga cccccgacca gaattgaaaa aacctagtta taagcgcatt ttgttcggtt
     4081 aactacagct aatataacat tgtggagcct atgacattga tttatgtctc ggtctcaaat
     4141 gttcttgtaa aaaaactaag tatagttgaa ttttaagtta aaaaaattgt ttttgcaaaa
     4201 ataaaataat ccatcaactg caaaaaaata tgcaatgatg gactaatgtc aaaaaattgt
     4261 tacatccatt ttattttagg ttcttcgcct ctaaaaatcc ttgcgatatt agagcgatgt
     4321 ctaattatca atatgattga aactaagaaa ctaacgacta ataaaatata gtcttgaatg
     4381 ataagcgagc caatcacaca gcaaattgct gcaacgatac ttgctaaaga aacatattta
     4441 aaaatcttca atacaataaa gaagataatt gcaagtatta gtaaaagtat cggattgact
     4501 cccaagacga cacctgcact agttgcaaca gctttgccac cttggaattt taaataaaca
     4561 ggataaacgt gtccaagtat agcgaataag ccaacaatta aaccatttgt aaaaaaagta
     4621 ctaatagggc catctgcgtg aacttgtaac cataaaggga agaaaacagt tatgaaccct
     4681 ttgaaaatat ctagaaatgt taccaagaat cctgcaggac gacctaatac tctaaagcta
     4741 ttagtagcgc cagtattacc actaccaaat tgtctaatat cttttttgaa aaataatttt
     4801 ccaattacga atccacttgg gaaagcgccg ataagataac ttagtagtaa catgacgatt
     4861 atcatcataa atattacaca tcctttaata tcttaggact attttatcat attcatttaa
     4921 attacggcta aaaattttaa aaacggggat taatatatgg aattaagcta tgaaagttaa
     4981 ttgatacttg cattttacgc tgatttatat aagaataact attgtatagt tttaaaaacg
     5041 aacgtacgtt tgcaggaggc gaaatcattg gcaatgaata aacaaaataa ttattcagat
     5101 gattcaatac aggttttaga ggggttagaa gcagttcgta aaagacctgg tatgtatatt
     5161 ggatcaactg ataaacgggg attacatcat ctagtatatg aaattgtcga taactccgtc
     5221 gatgaagtat tgaatggtta cggtaacgaa atagatgtaa caattaataa agatggtagt
     5281 atttctatag aagataatgg acgtggtatg ccaacaggta tacataaatc aggtaaaccg
     5341 acagtcgaag ttatctttac tgttttacat gcaggaggta aatttggaca aggcggctat
     5401 aaaacttcag gtggtcttca cggtgttggt gcttcagttg taaatgcatt gagtgaatgg
     5461 cttgaagttg aaatccatcg agatggtaat atatatcatc aaagttttaa aaacggtggt
     5521 tcgccatctt ctggtttagt gaaaaaaggt aaaactaaga aaacaggtac caaagtaaca
     5581 tttaaacctg atgacacaat ttttaaagca tctacatcat ttaattttga tgttttaagt
     5641 gaacgactac aagagtctgc gttcttattg aaaaatttaa aaataacgct taatgattta
     5701 cgcagtggta aagagcgtca agagcattac cattatgaag aaggaatcaa agagtttgtt
     5761 agttatgtca atgaaggaaa agaagttttg catgacgtgg ctacattttc aggtgaagca
     5821 aatggtatag aggtagacgt agctttccaa tataatgatc aatattcaga aagtatttta
     5881 agttttgtaa ataatgtacg tactaaagat ggtggtacac atgaagttgg ttttaaaaca
     5941 gcaatgacac gtgtatttaa tgattatgca cgtcgtatta atgaacttaa aacaaaagat
     6001 aaaaacttag atggtaatga tattcgtgaa ggtttaacag ctgttgtgtc tgttcgtatt
     6061 ccagaagaat tattgcaatt tgaaggacaa acgaaatcta aattgggtac ttctgaagct
     6121 agaagtgctg ttgattcagt tgttgcagac aaattgccat tctatttaga agaaaaagga
     6181 caattgtcta aatcacttgt gaaaaaagcg attaaagcac aacaagcaag ggaagctgca
     6241 cgtaaagctc gtgaagatgc tcgttcaggt aagaaaaaca agcgtaaaga cactttgcta
     6301 tctggtaaat taacacctgc acaaagtaaa aacactgaaa aaaatgaatt gtatttagtc
     6361 gaaggtgatt ctgcgggagg ttcagcaaaa cttggacgag accgcaaatt ccaagcgata
     6421 ttaccattac gtggtaaggt aattaataca gagaaagcac gtctagaaga tatttttaaa
     6481 aatgaagaaa ttaatacaat tatccacaca atcggggcag gcgttggtac tgactttaaa
     6541 attgaagata gtaattataa tcgtgtaatt attatgactg atgctgatac tgatggtgcg
     6601 catattcaag tgctattgtt aacattcttc ttcaaatata tgaaaccgct tgttcaagca
     6661 ggtcgtgtat ttattgcttt acctccactt tataaattgg aaaaaggtaa aggcaaaaca
     6721 aagcgagttg aatacgcttg gacagacgaa gagcttaata aattgcaaaa agaacttggt
     6781 aaaggcttca cgttacaacg ttacaaaggt ttgggtgaaa tgaaccctga acaattatgg
     6841 gaaacgacga tgaacccaga aacacgaact ttaattcgtg tacaagttga agatgaagtg
     6901 cgttcatcta aacgtgtaac aacattaatg ggtgacaaag tacaacctag acgtgaatgg
     6961 attgaaaagc atgttgagtt tggtatgcaa gaggaccaaa gtattttaga taattctgaa
     7021 gtacaagtgc ttgaaaatga tcaatttgat gaggaggaaa tctagtgagt gaaataattc
     7081 aagatttatc acttgaagat gttttaggtg atcgctttgg aagatatagt aaatatatta
     7141 ttcaagagcg tgcattgcca gatgttcgtg atggtttaaa accagtacaa cgtcgtattt
     7201 tatatgcaat gtattcaagt ggtaatacac acgataaaaa tttccgtaaa agtgcgaaaa
     7261 cagtcggtga tgttattggt caatatcatc cacatggaga ctactcagtg tacgaagcaa
     7321 tggtccgttt aagtcaagac tggaagttac gacatgtctt aatagaaatg catggtaata
     7381 atggtagtat cgataatgat ccgccagcgg caatgcgtta cactgaagct aagttaagct
     7441 tactagctga agagttatta cgtgatatta ataaagagac agtttctttc attccaaact
     7501 atgatgatac gacactcgaa ccaatggtat tgccatcaag atttcctaac ttactagtga
     7561 atggttctac aggtatatct gcaggttacg cgacagatat accaccacat aatttagctg
     7621 aagtgattca agcaacactt aaatatattg ataatccgga tattacagtc aatcaattaa
     7681 tgaaatatat taaaggtcct gattttccaa ctggtggtat tattcaaggt attgatggta
     7741 ttaaaaaagc ttatgaatca ggtaaaggta gaattatagt tcgttctaaa gttgaagaag
     7801 aaactttacg caatggacgt aaacagttaa ttattactga aattccatat gaagtgaaca
     7861 aaagtagctt agtaaaacgt atcgatgaat tacgtgctga caaaaaagtc gatggtatcg
     7921 ttgaagtacg tgatgaaact gatagaactg gtttacgaat agcaattgaa ttgaaaaaag
     7981 atgtgaacag tgaatcaatc aaaaattatc tttataaaaa ctctgattta cagatttcat
     8041 ataatttcaa catggtcgct attagtgatg gtcgtccaaa attgatgggt attcgtcaaa
     8101 ttatagatag ttatttgaat caccaaattg aggttgttgc aaatagaacg aagtttgaat
     8161 tagataatgc agaaaaacgt atgcatatcg ttgaaggttt gattaaagcg ttgtcaattt
     8221 tagataaagt aatcgaattg attcgtagct ctaaaaacaa gcgtgacgct aaagaaaacc
     8281 ttatcgaagt atacgagttc acagaagaac aggctgaagc aattgtaatg ttacagttat
     8341 atcgtttaac aaatactgac atagttgcgc ttgaaggtga acataaagaa cttgaagcat
     8401 taatcaaaca attacgtcat attcttgata accatgatgc attattgaat gtcataaaag
     8461 aagaattgaa tgaaattaaa aagaaattca aatctgaacg actgtcttta attgaagcag
     8521 aaattgaaga aattaaaatt gacaaagaag ttatggtgcc tagtgaagaa gttattttaa
     8581 gtatgacacg tcatggatat attaaacgta cttctattcg tagctttaat gctagcggtg
     8641 ttgaagatat tggtttaaaa gatggtgaca gtttacttaa acatcaagaa gtaaatacgc
     8701 aagataccgt actagtattt acaaataaag gtcgttatct atttataccg gttcataaat
     8761 tagcagatat tcgttggaaa gaattgggac aacatgtatc acaaatagtt cctatcgaag
     8821 aagatgaagt ggttattaat gtctttaatg aaaaggactt taatacagat gcattttatg
     8881 tttttgcgac tcaaaatggc atgattaaga aaagtacagt gcctctattt aaaacaacgc
     8941 gttttaataa acctttaatt gctactaaag ttaaagaaaa tgatgatttg attagtgtta
     9001 tgcgctttga aaaagatcaa ttaattaccg tcattactaa taaaggtatg tcattaacgt
     9061 ataatacaag tgaactatca gataccggat taagggcagc tggtgttaaa tcaataaatc
     9121 ttaaagctga agatttcgtt gttatgacag aaggtgtttc tgaaaatgat actatattga
     9181 tggccacaca acgcggctcg ttaaaacgta ttagttttaa aatcttacaa gttgctaaaa
     9241 gagcacaacg tggaataact ttattaaaag aattaaagaa aaatccacat cgtattgtag
     9301 ctgcacatgt agtgacaggt gaacatagtc aatatacatt atattcaaaa tcaaatgaag
     9361 aacatggttt aattaatgat attcataaat ctgaacaata tacaaatggc tcattcattg
     9421 tagatacaga tgattttggt gaagtaatag acatgtatat tagctaaaaa ctatatgcaa
     9481 tcacgaaatt aaatgataaa atacagtaat gttaaatttt gactaaattc aagggattta
     9541 tattaaatgc tgaccaagta cttatcgtta aattagcgat atactttgac caaactaata
     9601 taaatctctt gtattgtata agaaatgatg atgtatctct atatttactg aaggttcaat
     9661 tcggtctaat tttatagaag gtacatgatt catgtagagg atgcgagggg tttattttga
     9721 aagatttcga tagtttaata cctggatggt ttaaagaatt tgtccatgtt ggtaccgatt
     9781 taatatggtc tcaatattta attggtctat tattgacagc tggattcttc tttacgatta
     9841 gttctaaatt cgttcaatta cgaatgttac ctgaaatgtt tagagcttta gtagaacgtc
     9901 cagaaacttt agaagatggt aagaagggta tttcgccatt ccaagcattt gcgattagtg
     9961 ctggttcgag agttggtact ggtaatattg ctggtgttgc gactgcgatt gttttaggcg
    10021 gtccaggtgc agtattttgg atgtgggtta ttgcatttat aggtgcagcg agtgcattta
    10081 tagaagcgac tttggctcag gtttataaag tacatgataa agatggtgga ttccgtggtg
    10141 gtccagctta ctatattact aaaggtttaa atcaaaaatg gctaggtatc gtatttgcga
    10201 ttttaattac aattacattt gcatttgtat ttaacacagt gcaatctaat acaattgcgg
    10261 agtcgttaaa tacgcaatat aatattagtc cagtaatcac aggtattatt ttagcaatcg
    10321 taacagctat tattatattt ggtggtgtac gtagtattgc tacgttatct tcgttaattg
    10381 taccgattat ggctatcatt tacattggta tggttttagt aatattgcta tttaatttag
    10441 atcaaattgt tcctatgata ggtacgatta ttaaaagtgc atttggtatc gaacaagtaa
    10501 ctggtggcgc tgtaggtgct gcggttcttc aaggtatcaa acgtggttta ttctctaacg
    10561 aagctggtat gggttctgcg ccgaatgcag cggcaactgc tgccgtacca caccctgtta
    10621 agcaaggttt aatccaatca ttaggtgtgt tctttgatac aatgttggtt tgtacagcaa
    10681 ctgcaatcat gattttacta tattcaggac tgaaatttgg tgataacgca cctcaaggtg
    10741 ttgcagttac tcaatcagca cttaatgagc atttaggttc tgctggaggt attttcttaa
    10801 caatagcagt tacactgttt gcattttcat ctgttgtagg taattactat tacggtcaat
    10861 ctaatattga atttttatca acaaaccgtg taatattatt tatctttaga tgtcttgttg
    10921 tagtacttgt ctttgtcggt gcagttgtaa aaacagaaac agtatggaat acggcagact
    10981 tatttatggg cttaatggca attgtaaaca ttatttccat tataggactg tccaatgtag
    11041 cttttgcatt gatgaaagat tatcaaaagc agaaaaaaga aggcaagaac cctgtcttta
    11101 aacctgaaaa cttagaaatt aacttatttg gaattagtgc ttggggcgct aacaaatata
    11161 agaactctga taaataaata tttttaatgt aacgattcat tatataaaaa tcaaataaac
    11221 cctgaggttt tcaaattaaa gcgaaaatct cagggtttta aatttgtact caattatcat
    11281 ctgtatcatt acactagtta atctaagatt tgattatata ttaatatagt aaggttgaga
    11341 tttatttcct ccattggtgt gcaaagacta taccattgat aagacatatt gttggataga
    11401 tgaatataaa tttgttttag ctttgcacaa tttaatagta cgttttagtt tgtattgagt
    11461 attaattcag ataatatata aataaataga attatttgta aatatgggag tcagattttt
    11521 ttattatagt tattccactt tgagagacat cgaaattaaa ttaagatata tcatatagga
    11581 taggcgtgta atagaacaca agtatttaaa ttattatact ttcaagttag acatttccga
    11641 tatataatct taataataga aaaataggtg atgataatgg gagaatatat tgttactaaa
    11701 acattgaaca acaatgtcgt agtatgtact aataatgatc aagaagttat tttaatcggt
    11761 aaaggtattg gttttaacaa aaaagaggga atggcgttaa acgaccaaac tattacaata
    11821 gagaaaattt ataaattaga aagtgagcaa caaaaagcac attataaaag tttagttgaa
    11881 atcgctgatg ataatgtatt acaagtaatt attgattcgt tgaattttat ttctaatact
    11941 gcgatgaatg ttgattcaaa acaacttgta gtttcattaa cggatcatat tatatttgct
    12001 tataaacgct taaaacaaaa tcaagttatt agcaatccat ttgttatgga aactatgcag
    12061 ttatatagtg atgcatatca tattgctaaa caggtgattg atcagttaaa tgcagcatta
    12121 gatgtacatt ttcctgaaga tgagatagga tttattgcat tacatattgc atctaataca
    12181 gaagatttat ctatgcatga gatgaccttg atcaataatg ttattaaaaa aggtatagat
    12241 atcattgaat cagaccttgt gacaactgtt gataaggaat cattacaata ccaacgtttt
    12301 ataaggcacg tacaattttt aattcgccga ttaagaagaa aagaatatat acatgcacaa
    12361 gatgattttg tgtctatgat taaaaatcac tatccgattt gctataacac agcatataaa
    12421 attttaacta tgatacaaaa acaatttgat gttaatatca gtgagtctga aattatatat
    12481 ttaacattac acattcatca ttttgaagaa aggattaatc aatcctaaat taagtgtatt
    12541 acacgggcgt ttcgttactg taaagggtat ttgtatggta tcattgatgt tagtaatttt
    12601 acataagtgg actgttttta tacaacatct tatgaatatt ttaagtggtg gtgaatctat
    12661 aattgaatga aaatgaaaag aatataagaa agaatttttt aaaatttacc gaatcacggt
    12721 atatgaagtt tgttggtggg aatgatttag tcttctcatt aatagcgcta gtattgttgg
    12781 gtattgttat ttttattttc gaaaaagtat catatgtttt tgatcctttt atcatcgttt
    12841 ttaagacgat agcagcacct atcatcgtct ctttaattct attctatcta tttaacccaa
    12901 tcgtaaatat gatggaacgt tatagaatac caagagttgc aggtatttct attatttatc
    12961 tagctgtagt aggtgttatt acgttaattg ttaatttatt gatacctatt attggttcgc
    13021 aagtagatag tttagttaaa aattcaccgc aatatctaga aaaattaatt aattctattg
    13081 ataaaatagc aaataatacg tttttctctt cgtattatag tcaaattaat gattggttaa
    13141 attctttacc taagaaaata ccatctatgt taagtgaatt tacagatggc tttgggtcta
    13201 aaattgcaac gtttgcagaa acgattgcta atattggcgt tgtgattgtc acaacaccat
    13261 ttgtactatt ctttatgctt aaagatggac atcacttcaa agaattttca acgaatatta
    13321 tgccaccgaa attccgaaaa gattttcatg atctacttga aaaaatgagt gttcaagttg
    13381 gttcatacat tcaaggacaa attatcgttt cattctgtat cggtatactg ttgtttatcg
    13441 gttattcggt tatcgggttg aaatatagct tagtattagc tagtattgcg gcagttacaa
    13501 gtgttgtacc atatttaggg cctactatag cgatttctcc agctattgta atagctgcta
    13561 taacatcgcc gtggatgctc ttaaaattag cagtagtatg gactttagta caatttgttg
    13621 aagggcactt catttcacca aatatcatgg gtaaaacact taagattcat ccacttacaa
    13681 tcattttcat tttactgtgt gcaggcaaat tgcttggtat tgtaggcgtt attttaggta
    13741 ttccgggata tgctatttta aaagtattag ttactcattt attccaatta tttaaacgtc
    13801 gatacaatcg tt
//
LOCUS       DAKTIO010000058        13714 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.058, whole genome shotgun sequence.
ACCESSION   DAKTIO010000058 DAKTIO010000000
VERSION     DAKTIO010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13714
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.058"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..1616
                     /gene="coa"
                     /locus_tag="O0293_001307"
     CDS             <1..1616
                     /gene="coa"
                     /locus_tag="O0293_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498789.1"
                     /note="Staphylocoagulase, like its paralog von Willebrand
                     factor binding protein Vwb, is a coagulase and a virulence
                     factor. It induces clotting, not by being an enzyme, but
                     by activating host prothrombin to generate fibrin; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="staphylocoagulase"
                     /protein_id="HCY0280196.1"
                     /translation="KYEYGDNIYKEAKDRLLEKVLREDQYLLERKKSQYEDYKQWYAN
                     YKKENPRTDLKMANFHKYNLEELSMKEYNELQDALKRALDDFHREVKDIKDKNSDLKT
                     FNAAEEDKATKEVYDLVSEIDTLVVSYYGDKDYGEHAKELRAKLDLILGDTDNPHKIT
                     NERIKKEMIDDLNSIIDDFFMETKQNRPKSITKYNPTTHNYKTNSDNKPNFDKLVEET
                     KKAVKEADDSWKKKTVKKYGETETKSPVVKEEKKVEEPQAPKVDNQQEVKTTAGKAEE
                     TTQPVAQPLVKIPQGTITGEIVKGPEYPTMENKTVQGEIVQGPDFLTMEQSGPSLSNN
                     YTNPPLTNPILEGLEGSSSKLEIKPQGTESTLKGTQGESSDIEVKPQATETTEASQYG
                     PRPQFNKTPKYVKYRDAGTGIREYNDGTFGYEARPRFNKPSETNAYNVTTHANGQVSY
                     GARPTQNKPSKTNAYNVTTHGNGQVSYGARPTQNKPSKTNAYNVTTHANGQVSYGARP
                     TYKKPSKTNAYNVTTHADGTATYGPRVTK"
     gene            complement(1798..1905)
                     /locus_tag="O0293_001308"
     CDS             complement(1798..1905)
                     /locus_tag="O0293_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280197.1"
                     /translation="MCVRTRLVSSSSARLSKAIIIAVIVVYHLDVRGLF"
     gene            complement(2219..3403)
                     /locus_tag="O0293_001309"
     CDS             complement(2219..3403)
                     /locus_tag="O0293_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HCY0280198.1"
                     /translation="MQEAYIVAYGRSAAAKAKQGALFHERPDDVAAKVLQGVLKRIDG
                     KFNKNMIEDVIVGTAFPEGLQGQNIARTIALRAGLSDTVPGQTVNRYCSSGLQTIAIA
                     ANQIMAGQGDILVAGGVELMSAVPMGGNEPTNNPTLQYDDIGASYPMGLTAENVASQF
                     DVSREDQDAYAVRSHQRAYDAQRDGRFKDEIIPIQVNSVEYTNAGPKVHTNIFDQDEF
                     IRPDTTMEALAKLRTVFKADGTVTAGTSAPLSDGAGFVVLMSGDKVKELGVTPIARFV
                     GFKAVGVDPKIMGIGPAYAIPEVLSLSNLSVEDIDLIELNEAFASQTIASIKEVGLDI
                     SRTNVNGGAIALGHPLGATGAMLTARLLNEMGRRPDSRYGMVTMCIGVGMGAAAIFEY
                     VR"
     gene            complement(3433..5694)
                     /locus_tag="O0293_001310"
     CDS             complement(3433..5694)
                     /locus_tag="O0293_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HCY0280199.1"
                     /translation="MTINKVTVLGAGTMGAQLAALFVNAGLKVKLLDIVVDKNDPNLI
                     AKKSYDKITDKKRPLLFDLNLASHLTYGNFDDDLVNDDADLYIEAVKEDIEIKHAVWQ
                     QVLQHAKEDALFATNTSGIPINAIAQAFNEKDQERFFGLHFFNPPRIMKLVELIPTSH
                     TKESIILDVKNFAQNVLGKGVIVVNDVPGFVANRVGTQTMNDIMYRAEQHKISIVDVD
                     ALTGQAIGRPKTGTYALSDLVGLDIAVSVIKGMQQVPEETPYFHDVKIVNTLFDNGAL
                     GRKTKQGFYKKDKETKARLVYDVEKQDYVPVSQPQLPILNEFNKDLVHNLDTIFNAQD
                     EAGLFLWETLRNNFYYSAINVPKATDDFRDIDRALVWGFNWKLGPFQLWDAMGYERVK
                     TRMEDELGDLPQWISDLDGGFYKQDETIEYATPISHFVKDELWDKGDAKLSVTHDDQL
                     LLKLQSKNNVITDEFNDALVDAIDLLENDHYTSMVIYADGNNFSVGANLFLMKKAHED
                     GLVDDVVAQSIDKLHYSFNRLKYSLKPVVTAVQGRALGGGCELVLYSPIVVAASETYI
                     GLVEAGVGLLPSGGGLAEMADRILRTSHKFDDKQASMTKVLTNIAFAKVSTNAFEARR
                     YGYLRDTDTIIFNTAQRVEVALKRAKYEAETNYIPNPRHQYIALGEDFKALIQGQLDA
                     QRRGHFISDHDYHIALNIATILAGGDLPRNTFINQRYIQSLEKIGFIDLLKSKKSYER
                     IAHMLKTGKPLRN"
     gene            complement(5880..7091)
                     /locus_tag="O0293_001311"
     CDS             complement(5880..7091)
                     /locus_tag="O0293_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HCY0280200.1"
                     /translation="MTFEKETVLKTLFPEDVLSIAKGLTDGEVEFLQQVDSLLESKYR
                     ENINQHWIDATVPEDYFKDLGELNYFNNPLLYKDRPNAKMPSQLFQFFMSYLLARFDI
                     SLATLLGVHQGLGHNTFYFGGSKEQIAKYVPKLQSHELRTCFALTEPEHGSDVAGGLE
                     TVAERQGDTWVINGEKKWIGGAHVSDVIPVFAVNKETGKPHCFVVRPEQDGVDIEVID
                     NKIALRIVPNALIKLTNVKVDEADRLQNITSFKDIAKILYSTRAGVAYMATGGMAGAL
                     RATLDYVTERKQFGKPISKYQLIQEKLAMMQGNLAQAMATCAQLANMQAHGEYDEVAT
                     STAKMMNALRLRETVAMGRGITGGNGILADDYDIARFFSDAEAIYTYEGTHEINALVI
                     GRALTGDSAFV"
     gene            complement(7203..8708)
                     /locus_tag="O0293_001312"
     CDS             complement(7203..8708)
                     /locus_tag="O0293_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HCY0280201.1"
                     /translation="MNFDWIKTRSDFDDDKPAVIDHAKQTSWTYQQLNARADNMAHYL
                     TSQGVKKGDVIGIFAPNDIAILDLLFACFKTGAVFLPLNWRLNPKEIAAIVEDAQLKL
                     LFYAEKHLSSLTDIDQNLLHMDIDVAQYDEIVNPDYHQPFQATPVEPQDLAALIYTSG
                     TTGSPKGVMFSYESFVHNGANLELTYKFNSNYITIVSTPMFHVLGFNDTVLPVLMSGG
                     TLILQRYFNGEELNDMIAQYHPTFIIMIPTMYYSTLRASNFNPENFRAMDYIIQGGSQ
                     PLPSIQAAFKQYGINIINGYGLTEAPLVLVNTPENSKRKPMSIGKAVMFVDARILDDN
                     GEEVPTGEIGELAIKAKNVTPGYWNKPAETAKAFHGRYLLTGDLAKMDNDGDIFIIDR
                     KKELIITGGENVLPSEVENALAEHPLVDRCVVVGYDHPKYGESIAAAIILREDEPHYA
                     EILNQHMRSRLAGYKVPRMYVPVTHMPLNSTQKPDKLAIRQMMNDKVSQTL"
     gene            complement(8734..10311)
                     /locus_tag="O0293_001313"
     CDS             complement(8734..10311)
                     /locus_tag="O0293_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HCY0280202.1"
                     /translation="MEVTCLKQITWHDLQHIIKDGDVIGLPALAVANLPAEVLRAVLA
                     QHDTYHTPNDLTFILANDIHSLGAAPDLDDFIERRMIKRVIMSILTASSKTAQAMKNN
                     DIEAYFLPQGIIATHYRQSNQLLPGVITKIGLNTAVDPRYGGGKVNTRTTDDLVSLVT
                     INDETYLHYTFPSVDVALLRGTYADQQGNIYLTQEAYLSECYHVALNAKANHGKVIVQ
                     VKALVDDYQLKPNEVVIPGNLVDYVYVTEDEKNHRQVIQSHYLPALSGEERIDGIPEP
                     ALPFNSRKLILRRAAQFLTYGDTISIGYGINNELSNLLHEECVEHDVQPILDVGIFGG
                     FVGSREHFGMNYNADVRMPHDRAWDFIYNNGVSVAYLSFAEVDQYGNVNVSYFNDRLN
                     GCGGFIDITQSVNKIIFSGTFVAGSHVSCHNQRLNIETEGQNQKFVSDVSHIDFNAQY
                     SQSLEQEIYFVTDRAVFELTNQGLKLIEIAPGLDLHKDILNQMAFKPIIADHLKLIDT
                     SIYKEKWGQLKQSIHKV"
     gene            10752..11894
                     /locus_tag="O0293_001314"
     CDS             10752..11894
                     /locus_tag="O0293_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HCY0280203.1"
                     /translation="MSSASTQSTKTSDIHNESIDKQMEAKAHETAQNTDLKNEARSLF
                     DNATKSIGRLAGNDESLNLNLKDMLSEVFKPHTKNEADEIFIAGTAKTTPAICDISEE
                     WGKPWLFSRVFIAFTVTFIGLWVMAAIFNNTNAIPGLIFIGALTVPLSGLFFFYESNA
                     FKNISIFEVIIMFFIGGVFSLLSTMVLYRFVVFSDQFERFGSLTFFDAFLVGLVEETG
                     KALIIVYFVNKLKTNKILNGLLIGAAIGAGFAVFESAGYILNFALGENVPLLDIVFTR
                     AWTAIGGHLVWSAIVGAAIVIAKEQHGFEFKDIFDKRFLIFFLSAVVLHGIWDTSLTV
                     LGSDTLKIFILIVIVWILVFILMGAGLKQVNLLQKEFKEQQKKVDE"
     gene            complement(12208..13683)
                     /locus_tag="O0293_001315"
     CDS             complement(12208..13683)
                     /locus_tag="O0293_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0280204.1"
                     /translation="MKFKRLATIFSAVLVLSGCGSMHSSGKDLNISLPLKTKSIAPYE
                     TDVPVKIGAAESLFKTNDQGKIEKALVKSYHQPNDTTLDIELKDNIKFQNGQKLTAEK
                     VKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTNSAYPELVSELANPFMAIYDTDA
                     KSDVNQTPVGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNL
                     ESQKDDLITDVPVNKVQDIENNQNLKVSKESGFRTSLLMYNHTNKKMTKSVREALDHI
                     IDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKMLLAKDGYTKEHPLK
                     IKLITYDGRPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSAWDATMYSFGTI
                     PRGDTGYFFNQAYKKDGAINKGDYNNSNVDDLINQLNHTVDVKERHNISNDIIKLSSR
                     DVPNSYIAYNDQIVAANSKVKNYKVTPEGIYLIDYRTTIER"
BASE COUNT         4406 a         2512 c         2364 g         4432 t
ORIGIN      
        1 ataaatatga atatggagat aatatttata aagaagctaa agataggttg ttggaaaagg
       61 tattaaggga agatcaatat cttttggaga gaaagaaatc tcaatatgaa gattataaac
      121 aatggtatgc aaattataaa aaagaaaatc ctcgtacaga tttaaaaatg gctaattttc
      181 ataaatataa tttagaagaa ctttcgatga aagaatacaa tgaactacag gatgcattaa
      241 agagagcact ggatgatttt cacagagaag ttaaagatat taaggataag aattcagact
      301 tgaaaacttt taatgcagca gaagaagata aagcaactaa ggaagtatac gatctcgtat
      361 ctgaaattga tacattagtt gtatcatatt atggtgataa ggattatggg gagcacgcga
      421 aagagttacg agcaaaactg gacttaatcc ttggagatac agacaatcca cataaaatta
      481 caaatgaacg tattaaaaaa gaaatgattg atgacttaaa ttcaattatt gatgatttct
      541 ttatggaaac taaacaaaat agaccgaaat ctataacgaa atataatcct acaacacata
      601 actataaaac aaatagtgat aataaaccta attttgataa attagttgaa gaaacgaaaa
      661 aagcagttaa agaagcagat gattcttgga aaaagaaaac tgtcaaaaaa tacggagaaa
      721 ctgaaacaaa atcgccagta gtaaaagaag agaagaaagt tgaagaacct caagcaccta
      781 aagttgataa ccaacaagag gttaaaacta cggctggtaa agctgaagaa acaacacaac
      841 cagttgcaca accattagtt aaaattccac agggcacaat tacaggtgaa attgtaaaag
      901 gtccggaata tccaacgatg gaaaataaaa cggtacaagg tgaaatcgtt caaggtcccg
      961 attttctaac aatggaacaa agcggcccat cattaagcaa taattataca aacccaccgt
     1021 taacgaaccc tattttagaa ggtcttgaag gtagctcatc taaacttgaa ataaaaccac
     1081 aaggtactga atcaacgtta aaaggtactc aaggagaatc aagtgatatt gaagttaaac
     1141 ctcaagcaac tgaaacaaca gaagcttctc aatatggtcc gagaccgcaa tttaacaaaa
     1201 cacctaaata tgttaaatat agagatgctg gtacaggtat ccgtgaatac aacgatggaa
     1261 catttggata tgaagcgaga ccaagattca ataagccatc agaaacaaat gcatataacg
     1321 taacaacaca tgcaaatggt caagtatcat acggagctcg tccgacacaa aacaagccaa
     1381 gcaaaacaaa cgcatataac gtaacaacac atggaaacgg ccaagtatca tatggcgctc
     1441 gcccaacaca aaacaagcca agcaaaacaa atgcatacaa cgtaacaaca catgcaaacg
     1501 gtcaagtgtc atacggagct cgcccgacat acaagaagcc aagtaaaaca aatgcataca
     1561 atgtaacaac acatgcagat ggtactgcga catatgggcc tagagtaaca aaataagttt
     1621 gtaactctat ccaaagacat acagtcaata caaaacatta cgtatcttta caacagtaat
     1681 catgcattct atgatgcttc taactgaatt aaagcatcga acaatcggaa gcatatttct
     1741 aaattattta ttcattatag tcttaaacat aacatgacct aatatattac taacctatta
     1801 aaataaacca cgcacatcta agtgatatac gacaatcaca gcaataataa ttgctttaga
     1861 aagtcgtgcc gaactggaac ttacaagtct agttcgaaca cacactgatg tgagtggttt
     1921 tctttatttt aaacatgaac aatcagataa gttactagca ttagcaaata ttattaaatc
     1981 aaagggcttc gattcataaa atttaaaaca atgattaaaa ttagacgtgt aaatgttaaa
     2041 ttctaaaacg gaaataacca ccatcccatt aaaccacttt tttgttcaat cactatattt
     2101 cacacagctt cattaataaa acgaaattgc ttcaacccgc ttcaacttca actggcttca
     2161 acttcagcct acttcattca ataacaaaac gaatccgctt catccaaaat caaccattct
     2221 aacgcacata ttcaaatata gcagctgcac ccatgccgac accaatacac atcgtaacca
     2281 tgccgtaacg gctatcggga cgtctaccca tttcattaag taaacgcgcg gttaacattg
     2341 cgcctgtagc acctaatgga tgacctaaag caatagcgcc accattcaca ttcgtacgtg
     2401 atatatctag acctacttct ttaatagatg caatcgtttg agaagcaaat gcttcgttca
     2461 attcgatcaa atcaatgtct tcaacagata gattgctgag tgacaatact tcaggaatcg
     2521 catatgcagg cccaataccc ataattttcg ggtcaacgcc tactgcctta aaaccaacga
     2581 atcgtgcaat aggtgtcacg ccgagttctt tcactttatc tccagacatt aaaactacaa
     2641 atcctgcacc atcagaaagt ggggcagatg ttcctgcagt cacagtgccg tcagctttaa
     2701 atactgtacg taatttggct aatgcctcca tcgtggtgtc agggcgtata aattcatctt
     2761 ggtcaaagat atttgtgtgt acttttggtc ctgcgtttgt atattcaact gagtttactt
     2821 gtattggaat aatttcatct ttgaaccgac catcacgttg tgcgtcatag gcacgttgat
     2881 gacttctgac agcataagca tcttgatctt cacgtgatac gtcaaattgg gatgctacat
     2941 tttcagcagt taaacccata ggatatgacg cacctatatc atcatattgt aaggttggat
     3001 tgtttgtggg ctcgttgcca cccattggta cggcactcat caattcaacg ccaccagcta
     3061 caagtatatc tccttgacca gccataattt gattggctgc aatcgcgatg gtttgtaatc
     3121 ctgatgagca gtagcgattc actgtttgac ccggtaccgt gtcagataat cccgcacgca
     3181 atgcaatcgt tcgtgcaatg ttttggcctt gtaatccttc tggaaaagcc gtaccaacaa
     3241 tgacatcttc aatcatattc ttattgaatt ttccgtcaat acgtttcaat acgccttgta
     3301 atactttggc tgcgacatca tcaggtcttt cgtggaataa tgcgccttgc tttgctttcg
     3361 ctgcggctga acgcccataa gctacaatgt atgcttcttg catggttatc atcctctctt
     3421 aatgactatc ttttaattac gtaatggctt accagttttt aacatatgtg caattctttc
     3481 atatgatttt ttagatttta gtaagtcaat aaagccaatt ttctccaacg attgaatgta
     3541 acgttgattg ataaatgtat ttcttggtaa atcaccaccc gctaaaattg tggcgatatt
     3601 taaggcaata tgataatcat ggtcgctaat aaaatgaccc cgtctttgcg catctaattg
     3661 tccttggatc aatgctttga agtcttcacc taaagcgata tattgatgtc taggattcgg
     3721 aatatagttt gtttctgctt catatttcgc acgtttgagc gcaacttcga cacgttgtgc
     3781 tgtattgaaa ataatcgtat ctgtatcacg taaataacca taacgacgtg cctcaaaggc
     3841 atttgtagag actttcgcaa atgcgatatt cgtcagtact tttgtcatgg aagcttgttt
     3901 gtcatcaaac ttatgcgatg tgcgtaatat gcgatcagcc atttctgcaa ggccaccgcc
     3961 actcggtaat aagccaacac ctgcttcaac aagaccgata tatgtttcac ttgcagcgac
     4021 aacaataggt gagtaaagta caagctcaca gccaccgcct aaggcacgac cttgaacagc
     4081 tgtgactact ggtttcaaac tatacttcaa acgattaaag ctataatgta atttatcaat
     4141 tgattgtgca acgacatcat ctacaagacc gtcttcatgc gcctttttca ttaagaaaag
     4201 gttagcaccc acactgaaat tgttaccatc tgcataaata accatacttg tgtaatggtc
     4261 attttccagt aaatcaatcg catcaactaa cgcatcgttg aattcatcgg taatgacatt
     4321 atttttactt tgtaatttca gtaacagttg atcatcatga gttacggaaa gtttggcatc
     4381 acctttatcc caaagttcat cttttacgaa gtgagaaata ggtgttgcat attcaatggt
     4441 ctcatcttgt ttataaaagc caccatctaa atcactaatc cattgtggta agtctccaag
     4501 ttcgtcttcc atacgtgttt taacacgttc gtatcccatt gcatcccata attggaatgg
     4561 accaagtttc cagttgaacc cccagacaag cgcacggtct atgtctcgga aatcatcggt
     4621 agctttaggt acattgatag cagagtaata gaaattatta cgtaatgtct cccataaaaa
     4681 tagtcccgct tcgtcttgcg cattgaatat ggtatcaagg ttatgcacta agtctttatt
     4741 aaattcattt aaaattggta attgtggttg cgatacaggt acataatctt gtttttcaac
     4801 atcgtaaaca agtcgagctt tagtttcttt atcctttttg taaaatcctt gtttcgtttt
     4861 acgtccgagt gcgccattgt caaacaacgt atttacaatt ttgacatcat gaaaataagg
     4921 tgtttcttca ggtacttgtt gcatgccttt aattacagac actgcaatat ctaaaccgac
     4981 taggtcagat agcgcatatg tacctgtttt aggacgacca atcgcttgcc cagttaaagc
     5041 atccacatct acaatgctta tcttgtgttg ctcggcgcga tacataatat cattcattgt
     5101 ttgcgtgccg actctatttg cgacaaagcc aggcacatca ttgacgacaa tgacaccttt
     5161 acctaacaca ttttgcgcga aattttttac atctaatata atagattcct tcgtgtgtga
     5221 cgtaggtatt aactccacta atttcataat acgtggtggg ttaaagaaat gtagaccaaa
     5281 gaatcgttct tgatccttct cgttaaatgc ttgagcaatc gcattaattg gaatacctga
     5341 tgtatttgta gcgaataaag catcttcttt agcatgttgt agaacttgtt gccaaacagc
     5401 atgcttaatt tcaatatctt ctttgactgc ttcgatatat aaatcagcat catcatttac
     5461 caagtcatca tcaaaattac catatgttaa atgactcgct agatttaagt cgaatagtag
     5521 cggccgtttc ttatctgtaa ttttatcgta agattttttc gcaatgagat ttggatcgtt
     5581 tttgtccact acaatatcta atagttttac tttaagtcca gcattcacaa aaagtgctgc
     5641 cagttgagcg cccattgtgc ctgcgccaag aacggttact ttattaattg tcatagtgat
     5701 tcctccaatt tagttgagga taagataacc attaagataa ttggaataac gttgctattt
     5761 tataaaatta attaagtatc tttgacagtc atcttagcct cttatttaag gaaaaagctt
     5821 tatgcttaaa ataagtcttt tttagtgaaa ttaatgcatc tcatataatt atttgctatt
     5881 tatacgaaag cagaatctcc agtcaaagcg cgtccaatta ctaaggcatt aatttcatgt
     5941 gtaccttcgt acgtgtaaat cgcttctgca tcagagaaga aacgtgcaat atcataatcg
     6001 tcagctagta tgccattacc acctgtaata ccgcggccca tagctactgt ctcacgcaaa
     6061 cgtaaggcat tcatcatctt cgccgttgaa gttgcaacct cgtcatattc accatgtgct
     6121 tgcatattag ctaattgagc acatgttgcc attgcttgag ctaaattacc ttgcatcatt
     6181 gctagctttt cttgtattaa ctgatattta ctaattggtt tgccgaattg cttacgctca
     6241 gtgacataat ctaatgtggc acgtaaagcg ccagccatac cacctgtagc catataagca
     6301 acgcctgctc tcgttgaata aagaattttg gcaatatctt taaagcttgt tatgttttgt
     6361 aagcgatccg cttcatctac tttgacatta gttaatttaa ttagggcgtt aggaacaatg
     6421 cgaagtgcga ttttattatc aatgacttca atatcgacgc catcttgttc tggtctgact
     6481 acaaagcaat ggggtttgcc agtttcttta tttactgcga atactggaat gacatcagat
     6541 acatgtgcac caccaatcca tttcttttca ccattgataa cccaagtatc gccttggcgt
     6601 tcagcgactg tttcaagacc tcccgcaacg tccgaaccgt gttctggttc agttaaagca
     6661 aagcatgtac gcagttcatg tgactgtaat ttaggtacat atttcgcaat ttgttctttg
     6721 ctacctccga aatagaaagt gttatgccct aaaccttggt gaacaccgag tagggtagct
     6781 aaggaaatat caaatcgcgc gagtaggtaa gacatgaaaa actgaaatag ttgactaggc
     6841 attttggcgt ttggacgatc cttgtaaagt aatggattgt taaaataatt taattctccc
     6901 agatctttaa aatagtcctc gggtacagta gcgtctatcc aatgttgatt aatattttca
     6961 cggtacttac tttctagcaa tgaatctact tgttgtaaaa attcgacttc accgtctgtt
     7021 aaacctttag caatactaag tacatcttca ggaaataatg tttttaagac cgtttctttt
     7081 tcaaatgtca tataaattcc tcctaaaaat aatatgaata ctaatgtgaa atgcatttaa
     7141 ttcaaaaaca acacgcttta tttgtaaacg cttacactaa acgtcaaaaa tttttatcac
     7201 ctttaaagtg tttgcgagac tttgtcattc atcatttgtc gaatcgcaag tttatctggt
     7261 ttctgcgtac tgtttaacgg catatgtgtc actggtacat acattcttgg gactttataa
     7321 cctgctaaac gacttcgcat atgttgattt aaaatttcag cgtaatgagg ttcatcttcg
     7381 cgaagtataa tggctgcagc aattgattca ccatattttg gatgatcata gccaacgacc
     7441 acacaccggt ctactagtgg atgctcagct aaagcatttt cgacttcgga tggtaagaca
     7501 ttttcgccac cagttatgat taattctttt ttgcggtcaa taataaatat atcgccatcg
     7561 ttgtccatct tcgctaagtc accagttaat aaatatcgac catgaaatgc tttggcagtc
     7621 tctgctggtt tattccaata tcctggcgtg acatttttag ccttaattgc aagttcgcca
     7681 atctcaccag taggtacttc ctcaccgtta tcatcaagga tacgtgcatc aacgaacatg
     7741 actgctttac caatactcat tggcttacgt tttgaatttt ccggtgtatt aacaagtaca
     7801 agaggtgctt cagttaaacc atagccgtta ataatgttta tgccatattg tttaaaagct
     7861 gcttggatac ttggtaatgg ttgtgaacca ccttggatga tataatccat agctctaaaa
     7921 ttttcaggat taaaattact agcacgtagc gtactataat acattgtcgg aatcatgata
     7981 ataaatgtag ggtgatattg tgcaatcatg tcattcaatt cttcgccgtt aaagtaacgt
     8041 tgaagaataa gtgtgccacc tgacattaat actggtaata cagtatcgtt aaaccctaaa
     8101 acatggaaca ttggtgttga tacaatcgta atatagtttg aattgaactt atacgtcagc
     8161 tctaagtttg caccgttatg aacaaatgat tcatatgaga acatcacacc tttaggtgat
     8221 ccggttgtac cacttgtata aattaatgct gcaagatctt gtggttcaac aggtgttgct
     8281 tgaaaaggtt ggtgataatc tggatttacg atttcatcat attgcgccac atcaatatcc
     8341 atatgcaata agttttggtc aatatcggtg agtgaactta aatgtttttc agcatagaag
     8401 agcagtttta attgtgcatc ttccacaatg gctgcaattt cttttgggtt aagccgccaa
     8461 ttcaatggta aaaaaaccgc acctgtttta aaacaagcaa acaataaatc taatattgca
     8521 atatcatttg gcgcaaaaat accgataaca tcgccttttt taacaccttg agatgttaaa
     8581 taatgtgcca tattatcagc gcgtgcattg agttgttggt atgtccaaga tgtttgtttt
     8641 gcgtgatcaa taacggcagg cttgtcatca tcgaagtctg aacgcgtttt tatccaatcg
     8701 aaattcatta gtataccccc tttagcttca ctttcatact ttatgaattg attgtttaag
     8761 ttgtccccat ttttctttgt aaatgctggt atcaattaat tttaaatgat cagcaataat
     8821 tggtttaaaa gccatttgat tcaaaatatc tttatgcaaa tcaagacctg gtgcaatttc
     8881 aattagtttc aagccttgat tggtgagttc gaatactgca cgatcagtaa caaaatagat
     8941 ttcttgctcg agtgattgtg aatattgtgc attaaagtcg atatggctca catctgatac
     9001 aaatttctgg ttttgtcctt cagtttcaat gtttaatcgt tgattatggc atgagacatg
     9061 actgccagct acaaaagtac ctgaaaagat aattttattt acagattgcg taatgtctat
     9121 aaagccacca catccattta gtcggtcatt gaagtaagac acgttgacat tgccgtattg
     9181 atcaacctca gcaaagctaa gataggcaac tgatacacca ttgttataaa taaaatccca
     9241 tgctcgatca tgaggcatgc gcacatctgc attgtaattc ataccaaaat gttcacgact
     9301 cccaacgaat ccaccgaaaa tgccaacatc taaaatcggt tgcacatcat gttcaacaca
     9361 ttcttcatgc aataaattag agagttcatt attgatgcca taaccgatgc taattgtatc
     9421 gccataagtt aaaaactgag cagcacgtcg gagaatcaat ttgcgactat taaaaggtaa
     9481 tgcgggttca ggtattccat caattcgttc ttctccagac aaggctggta aataatgact
     9541 ttgaattact tggcggtgat tcttttcatc ttctgtgacg tatacataat cgacaagatt
     9601 tcctgggata acaacttcat tcggttttag ttgatagtcg tcaactaaag ctttaacttg
     9661 tacaataact ttcccatgat tggctttcgc gtttaatgcg acatgataac actcgctcaa
     9721 gtacgcttct tgagttaaat aaatgttacc ttgttgatct gcgtatgttc ctctcagtag
     9781 tgccacatca acgctaggga atgtgtaatg taagtatgtt tcatcgttga tggttactaa
     9841 tgaaactaaa tcatccgttg ttcgtgtatt tactttaccg ccaccgtatc taggatcaac
     9901 agctgtgttt aatccgattt tagtaataac tccaggtaat aattgattac tctgacgata
     9961 atgagttgca atgatacctt gtggtaaaaa ataagcttca atgtcattat ttttcattgc
    10021 ttgtgccgtt ttggaagaag ccgttaaaat actcataatg acacgtttaa tcatgcgacg
    10081 ttctataaaa tcatctaaat ccggtgcggc acctaaacta tgaatatcat tcgctaatat
    10141 aaacgttaaa tcattgggcg tatgatatgt gtcatgttgc gctaacacag cacgtagaac
    10201 ttcggcgggt aagttggcta cagctaatgc tggtaaacca atcacatcac catctttaat
    10261 gatatgttgt aagtcgtgcc atgtgatttg tttcaagcaa gtcacctcca tcacatttga
    10321 taaaatatag cgtttttaca ctttgtgtaa acccttacaa gaaatataac ataacgacgt
    10381 ttaaaatcaa ttagaaatat ctttttattc tgataataga cacagtatag acacattttg
    10441 acggtcgata acaattgtaa tatcaagggt ttgtaatgaa ttgaatatca ttaaaatact
    10501 tatataaaaa tattgttcgg aatataaaaa gttaaatagg ttttgatttt taaatatgaa
    10561 atacaaagtg cccaatcgaa caaagtattt atattaaaat atggaaaatc catcaatatt
    10621 aaattaaaat agttttatta tgaaaagtga aagtaggtaa gtctatggaa ggtcttaatc
    10681 atcgaagaaa tacagaaaaa gaagagacaa cacaaacgca atcagttgca cctaatacag
    10741 gtgaagaggg gatgtcatca gcaagtacac aatcaactaa gacgtccgac atacataatg
    10801 aatctatcga taaacaaatg gaagctaaag cgcatgaaac agcgcaaaat acagatttaa
    10861 aaaacgaagc aagaagttta tttgataatg caaccaaatc aatcggtaga ctagcgggca
    10921 atgatgaaag cttaaatctt aatttaaaag atatgctttc tgaagtattt aagccgcata
    10981 ctaaaaacga agcagatgaa atatttatag cgggtactgc taaaactacg ccagcaattt
    11041 gtgacatatc agaagaatgg gggaagccat ggctcttttc tcgagtattc atcgctttca
    11101 cagtaacatt tattggatta tgggtcatgg cagcaatttt taataacact aacgcgattc
    11161 cgggtctcat ttttataggg gctttaacag taccattatc gggtttgttc ttcttttatg
    11221 aatcaaatgc gtttaaaaat attagcattt ttgaagttat tatcatgttc tttattggcg
    11281 gcgtattttc attactaagt acgatggtat tatatagatt tgtcgttttt agtgatcaat
    11341 tcgaaaggtt tggttcttta acatttttcg atgcattttt agtaggatta gttgaagaaa
    11401 ctggaaaagc actcattatt gtttatttcg tcaataaatt gaaaacaaat aagattttga
    11461 atggattatt aatcggtgct gctattggtg cagggttcgc agtttttgaa tcagcaggtt
    11521 atattttgaa tttcgcttta ggagaaaatg tcccattatt agatattgtc ttcacacgtg
    11581 cgtggactgc gattggtggt catttagttt ggtcagcgat tgttggtgct gcaatagtta
    11641 ttgcgaaaga acagcatggc tttgaattca aagatatttt tgataaacgc tttttaatat
    11701 tctttttatc agccgttgtt ttacatggca tttgggatac atctttaact gtacttggca
    11761 gtgatacgtt gaaaatattt attttaatcg ttattgtgtg gatacttgta ttcattttaa
    11821 tgggggcagg tttaaaacaa gtgaatttac tgcagaaaga atttaaagaa caacagaaaa
    11881 aagtagacga ataataatta aagcttatgt tgctcatatg tttgtgacat aagctatttt
    11941 tataatttgt ctttaaaaga gtggaatagg aatacttttt ggagttaaaa aagtgtttca
    12001 cgttaaacaa atagtgacaa ttagatttat ataaaatgaa catgattcac tgaaagtatg
    12061 taataatcat tttattgaaa ttcatcaaac agaaattaat acaatcatat aagcaaatta
    12121 aaccacgcca taatcatatt ggatgacttc ggcgtggttt ttatagttga agcagggctg
    12181 agacataaat caatgtccca cactccctta tcgttcaatc gttgttcgat aatcgattaa
    12241 atagatacct tcaggtgtta ctttataatt tttaacctta gagttagcag cgactatttg
    12301 atcgttgtaa gcaatataac tgtttggtac atctcgactt gataatttaa taatatcatt
    12361 agaaatattg tgacgttcct taacatctac agtatgattc aattgattaa ttaaatcatc
    12421 gacgttgcta ttattgtagt ctcctttatt aatagcacca tcttttttat atgcttgatt
    12481 aaagaaataa cctgtatctc cacgaggaat tgttccgaaa ctatacatcg ttgcatccca
    12541 tgcagaacgg tcttttaagt aaccttctat gtcatcaaca cttttaatgt cgatttcaat
    12601 atttgctttt ttagcatctg attgtaatac ttgcgcaatt ttcgatagct ctggacgacc
    12661 gtcatacgta attaacttaa tttttaaagg gtgttctttt gtataaccat ctttagctaa
    12721 taacattttt gcttgttcga tattttgttt ggttaactta ggttctttaa tatatggaat
    12781 tttatcatta aatggactcg ttgcaggttt cgcataacct tgataaatat gatctgcaat
    12841 accttgtcta tcaatgatat gatctaatgc ttcacgaacg gatttagtca tttttttatt
    12901 agtatgatta tacataagta aagaagttct aaatccagat tcttttgaca cttttaaatt
    12961 ttgattattt tctatgtctt gaactttatt aactgggaca tcagttatta aatcatcttt
    13021 ttgagattct aaatttctga cgcgattatt gccgtcttct tggtacgtca cagtaatatg
    13081 atcaagtttc ggtttacctt gccaatagtc cttaaaattc gacaatgata tttttcgaga
    13141 ttgcttataa tcttttattt ggtaagggcc tgtaccaaca ggagtttgat taacatctga
    13201 tttagcatct gtatcataaa ttgccataaa aggattagct aattcagata caagttcagg
    13261 gtaagcggag ttggttttaa ttgtcagttt ttgaccttta gcggtaattg atgatattgg
    13321 taatgaatat ttgaccaagt cgcttttttt catgctattt tcaaggctag atttcacttt
    13381 ttctgcagtc aatttttgac cgttttgaaa tttaatatta tcttttaatt ctatatctaa
    13441 cgttgtatca tttggttgat gatacgattt cactaatgct ttttctattt ttccttgatc
    13501 atttgtttta aataatgatt ctgcagcacc aatcttaact ggtacatctg tttcataagg
    13561 tgcaatagac tttgttttta acggtaacga aatatttaag tctttgccag atgaatgcat
    13621 tgagccacat cctgataaca ctaatactgc tgaaaatata gttgctagtc ttttaaactt
    13681 catttcatta acactctctt tctaattact atgt
//
LOCUS       DAKTIO010000059        13706 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.059, whole genome shotgun sequence.
ACCESSION   DAKTIO010000059 DAKTIO010000000
VERSION     DAKTIO010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13706
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.059"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            69..362
                     /gene="esxA"
                     /locus_tag="O0293_001316"
     CDS             69..362
                     /gene="esxA"
                     /locus_tag="O0293_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxA"
                     /protein_id="HCY0280205.1"
                     /translation="MAMIKMSPEEIRAKSQSYGQGSDQIRQILSDLTRAQGEIAANWE
                     GQAFSRFEEQFQQLSPKVEKFAQLLEEIKQQLNSTADAVQEQDQQLSNNFGLQ"
     gene            446..3475
                     /gene="esaA"
                     /locus_tag="O0293_001317"
     CDS             446..3475
                     /gene="esaA"
                     /locus_tag="O0293_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaA"
                     /protein_id="HCY0280206.1"
                     /translation="MKKKNWIYALIVTLIIIIAIVSMIFFVQTKYGDQSEKGSQSVSN
                     KNNKIHIAIVNEDQPTTYNGKKVELGQAFIKRLANEKNYKFETVTRNVAESGLKNGGY
                     QVMIVIPENFSKLAMQLDAKTPSKISLQYKTAVGQKEEVAKNTEKVVSNVLNDFNKNL
                     VEIYLTSIIDNLHNAQKNVGAIMTREHGVNSKFSNYLLNPINDFPELFTDTLVNSISA
                     NKDITKWFQTYNKSLLSANSDTFRVNTDYNVSTLIEKQNSLFDEHNTAMDKMLQDYKS
                     QKDSVELDNYINALKQMDSQIDQQSSMQDTGKEEYKQTVKENLDKLREIIQSQESPFS
                     KGMIEDYRKQLTESLQDELANNKDLQDALNSIKMNNAQFAENLEKQLHDDIVKEPDTD
                     TTFIYNMSKQDFIAAGLNEDEANKYEAIVKEAKRYKNEYNLKKPLAEHINLTDYDNQV
                     AQDTSSLINDGVKVQRTETIKSNDINQLTVATDPHFNFEGDIKINGKKYDIKDQSVQL
                     DTSNKEYKVEVNGVAKLKKDAEKDFLKDKTMHLQLLFGQANRQDEPNDKKATSVVDVT
                     LNHNLDGRLSKDALSQQLSALSRFDAHYKMYTDTKGREDKPFDNKRLIDMMVDQVIND
                     MESFKDDKVAVLHQIDSMEENSDKLIDDILNNKKNTTKNKEDISKLIDQLENVKKTFA
                     EEPQEPKIDKGKNDEFNTMSSNLDKEISRISEKSTQLLSDTQESKSIADSVSGQLNQV
                     DNNVNKLHATGRALGVRANDLNRQMAKNDKDNELFAKEFKKVLQNSKDGDRQNQALKA
                     FMSNPVQKKNLENVLANNGNTDVISPTLFVLLMYLLSMITAYIFYSYERAKGQMNFIK
                     DDYSSKNHLWNNVITSGVIGTTGLVEGLIVGLIAMNKFHVLAGYRAKFILMVILTMMV
                     FVLINTYLLRQVKSIGMFLMIAALGLYFVAMNNLKAAGQGVTNKISPLSYIDNMFFNY
                     LNAEHPIGLVLVILTVLVIIGFVLNMFIKHFKKERLI"
     gene            3475..3933
                     /gene="essA"
                     /locus_tag="O0293_001318"
     CDS             3475..3933
                     /gene="essA"
                     /locus_tag="O0293_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HCY0280207.1"
                     /translation="MLMNSVIALTFLTASSNNGGLNIDVQQEEEKRINNDLNQYDTTL
                     FNKDSKAVNDAIAKQKKERQQQIKNDMFQNQASHSTRLNETKKVLFSKSNLEKTSESD
                     KSPYIQNKQEKKIFPYILMSVGAFLTLGFVIFSIHKGRRTKNESARKSNI"
     gene            3905..4147
                     /gene="esaB"
                     /locus_tag="O0293_001319"
     CDS             3905..4147
                     /gene="esaB"
                     /locus_tag="O0293_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaB"
                     /protein_id="HCY0280208.1"
                     /translation="MNQHVKVTFDFTNYNYGTYDLAVPAYLPIKNLIALVLDSLDISI
                     FDVNTQIKVMTKGQLLVENDRLIDYQIADGDILKLL"
     gene            4160..5494
                     /gene="essB"
                     /locus_tag="O0293_001320"
     CDS             4160..5494
                     /gene="essB"
                     /locus_tag="O0293_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HCY0280209.1"
                     /translation="MVKNHNPKNEMQDMLTPLDAEEAAKTKLRLDMREIPKSSIKPEH
                     FHLMYLLEQHSPYFIDAELTELRDSFQIHYDINDNHTPFDNIKSFTKNEKLRYLLNIK
                     NLEEVNRTRYTFVLAPDELFFTRDGLPIAKTRGLQNVVDPLPVSEAEFLTRYKALVIC
                     AFNEKQSFDALVEGNLELHKGTPFETKVIEAATLDLLTAFLDEQYQKQEQDYSQNYAY
                     VRKVGHTVFKWVAIGMTTLSVLLIAFLAFLYFSVMKHNERIEKGYQAFVKDDYTQVLN
                     TYDDLDGKKLDKEALYIYAKSYIQTNKQGLEKDKKENLLNNVTPNSNKDYLLYWMELG
                     QGHLDEAINIATYLDDNDITKLALINKLNEIKNNGDLSNDKRSEETKKYNDKLQDILD
                     KEKQVKDEKAKSEEEKAKAKDEKLKQQEENEKKQKEQAQKDKEKRQEAERKK"
     gene            5516..9955
                     /gene="essC"
                     /locus_tag="O0293_001321"
     CDS             5516..9955
                     /gene="essC"
                     /locus_tag="O0293_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HCY0280210.1"
                     /translation="MHKLIIKYNKQLKMLNLRDGKTYTISEDERADITLKSLGEVIHL
                     EQNNQGTWQANHTSINKVLVRKGDLDDITLQLYTEADYASFAYPSIQDTMTIGPNAYD
                     DMVIQSLMNAIIIKDFQSIQESQYVRIVHDKNTDVYINYELQEQLTNKAYIGDHIYVE
                     GIWLEVQADGLNVLSQNTVASSLIRLTQEMPHAQADDYNTYHRSPRIIHREPTDDIKI
                     ERPPQPIQKNNTVIWRSIIPPLVMIALTVVIFLVRPIGIYILMMIGMSTVTIVFGITT
                     YFSEKKKYNKDVEKREKDYKAYLDNKSKEINKAIKAQRFSLNYHYPTVAEIKDIVETK
                     APRIYEKTSHHHDFLHYKLGIANVEKSFKLDYQEEEFNQRRDELFDDAKELYEFYTDV
                     EQAPLINDLNHGPIAYIGARHLILEELEKMLIQLSTFHSYHDLEFLFVTREDEVETLK
                     WARWLPHMTLRGQNIRGFVYNQRTRDQILTSIYSMIKERIQAVRERSRSNEQIIFTPQ
                     LVFVITDMSLIIDHVILEYVNQDLSEYGISLIFVEDVIESLPEHVDTIIDIKSRTEGE
                     LITKEKELVQLKFTPENIDNVDKEYIARRLANLIHVEHLKNAIPDSITFLEMYNVKEV
                     DQLDVVNRWRQNETYKTMAVPLGVRGKDDILSLNLHEKAHGPHGLVAGTTGSGKSEII
                     QSYILSLAINFHPHEVAFLLIDYKGGGMANLFKDLVHLVGTITNLDGDEAMRALTSIK
                     AELRKRQRLFGEHDVNHINQYHKLFKEGIATEPMPHLFIISDEFAELKSEQPDFMKEL
                     VSTARIGRSLGIHLILATQKPSGVVDDQIWSNSKFKLALKVQDRQDSNEILKTPDAAD
                     ITLPGRAYLQVGNNEIYELFQSAWSGATYDIEGDKLEVEDKTIYMINDYGQLQAINKD
                     LSGLEDEETKENQTELEAVIDHIESITTRLEIEEVKRPWLPPLPENVYQEDLVETDFR
                     KLWSDDAKEVELTLGLKDVPEEQYQGPMVLQLKKAGHIALIGSPGYGRTTFLHNIIFD
                     VARHHRPDQAHMYLFDFGTNGLMPVTDIPHVADYFTVDQEDKIAKAIRIFNDEIDRRK
                     KILSQYRVTSISEYRKLTGETIPHVFILIDNFDAVKDSPFQEVFENMMIKMTREGLAL
                     DMQVTLTASRANAMKTPMYINMKTRIAMFLYDKSEVSNVVGQQKFAVKDVVGRALLSS
                     DDNVSFHIGQPFKHDETKSYNDQINDEVSAMTEFYKGETPNDIPMMPDEIKYEDYRES
                     LNLPDIVANGALPIGLDYEGVTLQKIKLTEPAMISSENPREIAHIAEIMMKEIDILNE
                     KYAICIADSSGEFKAYRHQVANFAEEREDIKAIHQLMIEDLKQREMDGPFEKDSLYII
                     NDFKTFIDCTYIPEDDVKKLITKGPELGLNILFVGIHKELIDAYDKQIDVARKMINQF
                     SIGIRISDQQFFKFRFIQREPVIKENEAYMVANQAYQKIRWFK"
     gene            9985..10377
                     /gene="esaC"
                     /locus_tag="O0293_001322"
     CDS             9985..10377
                     /gene="esaC"
                     /locus_tag="O0293_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion substrate EsaC"
                     /protein_id="HCY0280211.1"
                     /translation="MNFNDIETMVKSKFKDIKKHAEEIAHEIEVRSGYLRKAEQYKRL
                     EFNLSFALDDIESTAKDVQTAKSSANKDSVTVKGKAPNTLYIEKRNLMKQKLEMLGED
                     IDKNKESLQKAKEIAGEKASEYFNKAMN"
     gene            10393..10707
                     /gene="esxB"
                     /locus_tag="O0293_001323"
     CDS             10393..10707
                     /gene="esxB"
                     /locus_tag="O0293_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxB"
                     /protein_id="HCY0280212.1"
                     /translation="MGGYKGIKADGGKVDQAKQLAAKTAKDIEACQKQTQQLAEYIEG
                     SDWEGQFANKVKDVLLIMAKFQEELVQPMADHQKAIDNLSQNLAKYDTLSIKQGLDRV
                     NP"
     gene            10707..11381
                     /locus_tag="O0293_001324"
     CDS             10707..11381
                     /locus_tag="O0293_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5081 family protein"
                     /protein_id="HCY0280213.1"
                     /translation="MKDVKRIDYFSYEELTILGGSKLPLVNFELFDPSNFEEAKAALI
                     EKELVTENDKLTDAGFKVATLVREYISAIVNIRINDMYFAPFSYEKDEYILLSRFKNN
                     GFQIRIINKDIAWWSIVQSYPLLMRQEKSNDWDFKQIDDETLENLNNESIDTIGRVLE
                     IEIYNHQGDPQQSLYNIYEQNDLLFIRYPLKDKVLNVHIGVINTFIRELFGFDTDENH
                     INKAEE"
     gene            11381..11698
                     /locus_tag="O0293_001325"
     CDS             11381..11698
                     /locus_tag="O0293_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280214.1"
                     /translation="MTLSGKISVKAETIAHVVKELESISQKYDEIAQNFGKIAQLNYY
                     SSEKAAHSMENGYSSAATVISGLKGPLSTLGGGVMNSAQKFFEADEHWGTEFAKLYYN
                     IEG"
     gene            11708..13552
                     /locus_tag="O0293_001326"
     CDS             11708..13552
                     /locus_tag="O0293_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease YeeF family protein"
                     /protein_id="HCY0280215.1"
                     /translation="MTKDIEYLTADYDNEKSSIQSVIDAIEGQDFLDVDTTMDDAVSD
                     VSSLDEDGAISLTSSVVGPQGSKLMGYYQNELYDYASQLDSKMKEIIDTPFIEDIDKA
                     FKGITNVKLENILIKNGGGHGRDTYGASGKIAKGDAKKSDSDVYSIDEILKSDQEFVK
                     VIDQHYKEMKKEDKKLSKSDFEKMMTQGASCDYMTVAEAEELEEQKKKEEAIEIAALA
                     GMVVLSCINPVAGAVAIGAYSAYSAANAATGKNIVTGRKLSKEERIMEGLSLIPLPGM
                     GFLKGAGKSLMKLGFKGGEKFAVKTGLQKTMQQAVSRISPKMGMMKNSVLNQSRNFAQ
                     NTHVGQMLSNMRGQATHTVQQSRNWIGQQAQNVKRIVNNGLDKEIAHPFKQQLAPAGM
                     GGIKFAETTTLRNMGQNIKRAVTPQNHVTHGPKDSMVRSEGKHSISSHEMNSSKYVES
                     PNYTKVEFGEHYARLRPKKLKANIEYTTPTGHIYRTDHKGRIKEVYVDNLSLKDGDRN
                     SHAQRTVGGEDRLPDDDGGHLIARMFGGSKDIDNLVAQSKFINRPFKEKGHWYNLEKE
                     WQEFLNSGKEVKNIKMEVKYSGNSQRPTIFKVEYEINGERNIRRILNK"
     gene            13563..>13706
                     /locus_tag="O0293_001327"
     CDS             13563..>13706
                     /locus_tag="O0293_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF600 family protein"
                     /protein_id="HCY0280216.1"
                     /translation="MTFEEKLSKIYNEIANEISSMIPVEWEKVYTMAYIDDGGGEVFF
                     NYTK"
BASE COUNT         5341 a         1911 c         2608 g         3846 t
ORIGIN      
        1 cactttttca aaaaatagtg tccctaaaaa gttttgataa acttaaaata ttcaggaggt
       61 ttctagttat ggcaatgatt aagatgagtc cagaggaaat cagagcaaaa tcgcaatctt
      121 acgggcaagg ttcagaccaa atccgtcaaa ttttatctga tttaacacgt gcacaaggtg
      181 aaattgcagc gaactgggaa ggtcaagctt tcagccgttt cgaagagcaa ttccaacaac
      241 ttagtcctaa agtagaaaaa tttgcacaat tattagaaga aattaaacaa caattgaata
      301 gcactgctga tgccgttcaa gaacaagacc aacaactttc taataatttc ggtttgcaat
      361 aagcattctg aaattggcaa agtcacattt tctaatgtgg ctttgcttat cattttttta
      421 agaaaacaac tgaaaggaaa taagcatgaa aaagaaaaat tggatttatg cattaattgt
      481 cactttaatt attataattg ccatagttag tatgatattt tttgttcaaa caaaatatgg
      541 agatcaatca gaaaaaggat cccaaagtgt aagtaataaa aataataaaa tacatatcgc
      601 aattgttaac gaggatcaac caacgacata taacggtaaa aaagttgagc tgggtcaagc
      661 atttattaaa aggttagcaa atgagaaaaa ctataaattt gaaacagtaa caagaaacgt
      721 tgctgagtct ggtttgaaaa atggtggata ccaagtcatg attgttatcc cagaaaactt
      781 ttcaaaattg gcaatgcaat tagacgctaa aacaccatcg aaaatatcgc tacagtataa
      841 aacagctgta ggacaaaaag aagaagtagc taaaaacaca gaaaaagttg taagtaatgt
      901 acttaacgac tttaacaaaa acttagtcga aatttattta acaagcatca ttgataattt
      961 acataatgca caaaaaaatg ttggcgctat tatgacgcgt gaacatggtg tgaatagtaa
     1021 attctcgaat tacttattaa atccaattaa cgacttcccg gaattattta cagatacgct
     1081 tgtaaattca atttctgcaa acaaagacat tacaaaatgg ttccaaacat acaataaatc
     1141 attattgagt gcgaattcag atacgttcag agtgaacaca gattataatg tttcgacttt
     1201 aattgaaaaa caaaattcat tatttgacga gcacaataca gcgatggata aaatgttaca
     1261 agattataaa tcgcaaaaag atagcgtgga acttgataac tatatcaatg cattaaaaca
     1321 gatggacagc caaattgatc aacaatcaag tatgcaagat acaggtaaag aagaatataa
     1381 acaaactgtt aaagaaaact tagataaatt aagagaaatc attcaatcac aagagtcacc
     1441 attttcaaaa ggtatgattg aagactatcg taagcaatta acagaatcac tgcaagatga
     1501 gcttgcaaat aacaaagact tacaagatgc gctaaatagc attaaaatga acaatgctca
     1561 attcgctgaa aacttagaga aacaacttca tgatgatatt gtcaaagaac ctgatacaga
     1621 tacaacattt atctataaca tgtctaaaca agactttata gctgcaggtt taaatgagga
     1681 tgaagctaat aaatacgaag caattgtcaa agaagcaaaa cgttataaaa atgaatataa
     1741 tttgaaaaaa ccgttagcag aacacattaa tttaacagat tacgataacc aagttgcgca
     1801 agacacaagt agtttgatta atgatggtgt caaagtgcaa cgtactgaaa cgattaaaag
     1861 taatgatatt aatcaattaa ctgttgcaac agatcctcat tttaattttg aaggcgacat
     1921 taaaattaat ggtaaaaaat atgacattaa ggatcaaagt gttcaactcg atacatctaa
     1981 caaggaatat aaagttgaag tcaatggcgt tgctaaattg aaaaaggatg ctgagaaaga
     2041 tttcttaaaa gataaaacaa tgcatttaca attgttattt ggacaagcaa atcgtcaaga
     2101 tgaaccaaat gataagaaag caacgagtgt tgtggatgta acattgaatc ataaccttga
     2161 tggtcgctta tcgaaagatg cattaagcca gcaattgagt gcattatcta ggtttgatgc
     2221 gcattataaa atgtacacag atacaaaagg cagagaagat aaaccattcg acaacaaacg
     2281 tttaattgat atgatggttg accaagttat caatgacatg gaaagtttca aagacgataa
     2341 agtagctgtg ttacatcaaa ttgattcaat ggaagaaaac tcagacaaac tgattgatga
     2401 cattttaaat aacaaaaaga atacaacaaa aaataaagaa gatatttcca agctgattga
     2461 tcagttagaa aacgttaaaa agacttttgc tgaagagcca caagaaccaa aaattgataa
     2521 aggcaaaaat gatgaattta atacgatgtc ttcaaattta gataaagaaa ttagtagaat
     2581 ttctgaaaag agtacgcaat tgctatcaga tacacaagaa tcaaaatcaa ttgcagattc
     2641 tgttagtggc caattaaatc aagtcgacaa taatgtgaat aagctacatg cgacaggtcg
     2701 agcattaggc gtaagagcta acgatttgaa tcgtcaaatg gctaaaaacg ataaagataa
     2761 tgagttgttc gctaaagaat ttaaaaaagt attacaaaat tctaaagatg gcgacagaca
     2821 aaaccaagca ttaaaagcat ttatgagtaa tccggttcaa aagaaaaact tagaaaatgt
     2881 tttagctaat aatggtaata cagacgtgat ttcaccgaca ttattcgtat tattgatgta
     2941 tttactatca atgattacag catatatttt ctatagttat gaacgtgcca aaggacaaat
     3001 gaatttcatt aaagatgatt atagtagtaa aaaccatctt tggaataatg tcattacgtc
     3061 aggtgttatt ggtacaactg gtttggtaga agggttaatt gtcggtttaa ttgcaatgaa
     3121 taagttccat gtattagctg gctatagagc gaaattcatc ttaatggtga ttttaactat
     3181 gatggtcttc gtacttatta atacgtattt actaagacag gtaaaatcta tcggtatgtt
     3241 cttaatgatt gctgcattgg gtctatactt tgtagctatg aataatttga aagcagctgg
     3301 acaaggtgtg actaataaaa tttcaccatt gtcttatatc gataacatgt tcttcaatta
     3361 tttaaatgca gagcatccta taggcttggt gctagtaata ttaacagtac ttgtgattat
     3421 tggctttgta ctgaacatgt ttataaaaca ctttaagaaa gagagattaa tctaatgttg
     3481 atgaatagcg tgattgcttt aactttttta acagcatcta gcaataatgg cggacttaat
     3541 attgatgtgc aacaagaaga ggaaaagcga atcaataatg atttaaatca atatgataca
     3601 acgctattta ataaagacag caaagcggtt aatgatgcga ttgctaagca gaaaaaagaa
     3661 cgacaacaac aaataaaaaa tgatatgttt caaaatcaag cgagtcactc gactcgcttg
     3721 aatgaaacta aaaaagtgtt attttccaaa tctaacttag aaaagacttc ggagagtgat
     3781 aaaagcccct atattcaaaa caagcaggag aaaaaaatat tcccgtacat tttgatgtct
     3841 gtaggggctt ttttgacttt aggatttgtc attttttcaa ttcataaagg gagacgaacg
     3901 aaaaatgaat cagcacgtaa aagtaacatt tgattttact aattataatt acggcacata
     3961 tgacttagca gtaccagcat atttaccgat aaaaaactta atagctttag tattggatag
     4021 tttggacatt tcaatatttg atgtcaatac acaaattaaa gtgatgacga aaggtcaatt
     4081 acttgttgaa aatgatcgac tcattgatta tcaaatcgct gatggagata ttttgaagtt
     4141 actataggag gaaaaataga tggttaaaaa tcataaccct aaaaatgaaa tgcaagatat
     4201 gttaacgcct ttagatgctg aagaagcagc taaaacaaaa ttacgcttag atatgagaga
     4261 gattcctaag tcttcaatta aaccagaaca ttttcattta atgtacttat tagaacaaca
     4321 ttctccatat tttatagatg ctgaattaac tgaactacgt gacagtttcc aaatacatta
     4381 tgacattaat gacaatcata caccttttga taatattaaa tcatttacta aaaatgaaaa
     4441 attacgttac ttactcaata tcaaaaattt agaagaagta aatcgtacac gctacacatt
     4501 tgtgttggca ccagatgaat tatttttcac aagagatgga ttacccattg ctaaaacaag
     4561 agggttacaa aatgttgttg atccattacc tgtgtcagaa gctgaatttt taacaagata
     4621 taaagcgctg gttatctgtg cattcaatga gaaacaatca tttgatgctt tagttgaagg
     4681 aaacttagaa ctacataaag gaacgccatt tgaaactaaa gttattgaag cggcaacgtt
     4741 agatttacta acggcatttt tagatgaaca gtatcagaaa caagaacaag attatagtca
     4801 aaattatgca tatgtacgca aagtaggaca taccgttttc aaatgggttg ctatcggtat
     4861 gacaacgtta agtgttttat taattgcatt cttagccttt ttatattttt cagtaatgaa
     4921 gcataatgag cgcattgaaa aaggatacca agcatttgta aaggatgatt atacgcaagt
     4981 actaaatacg tatgatgatt tagatggtaa aaaattagat aaagaggcac tttacattta
     5041 tgccaaaagt tatatccaaa caaataaaca aggtttagaa aaagataaga aagaaaattt
     5101 acttaataat gtgacaccaa attcaaacaa agactactta ttatattgga tggaattagg
     5161 acaaggacat cttgatgaag cgattaatat tgccacttat ttagatgata acgatattac
     5221 aaagttagcg ttgattaata aattaaatga gattaaaaat aacggagatt tatcgaatga
     5281 taaacgttct gaagaaacga aaaagtataa cgataaattg caagatattt tagacaaaga
     5341 aaaacaagtt aaagatgaaa aagcgaaatc tgaagaagag aaagcaaaag cgaaagatga
     5401 gaaattaaag caacaagaag agaacgaaaa gaaacaaaaa gaacaagcac aaaaagataa
     5461 agaaaaacgc caagaagctg aaagaaaaaa atagtatagg actgaggcaa agacaatgca
     5521 taaattgatt ataaaatata acaaacaatt gaagatgctc aatttgcgag atggtaagac
     5581 atatactatt agcgaagacg agcgtgcaga tattacgttg aaatcgttag gcgaagtcat
     5641 tcatttagaa caaaataatc aaggtacttg gcaagcgaat catacttcta ttaataaggt
     5701 gcttgttaga aaaggtgacc ttgatgacat tacattacag ctttatacag aagctgatta
     5761 tgcatcattt gcgtatcctt caattcaaga tacgatgaca attggaccaa atgcgtatga
     5821 tgatatggtt attcaaagct tgatgaatgc catcattatt aaagattttc aatcaataca
     5881 agaatcacaa tacgtacgca ttgtgcacga taaaaataca gatgtgtata ttaactatga
     5941 actacaagag caactaacga acaaagctta cattggtgat catatttatg ttgaagggat
     6001 atggctcgaa gtacaagctg atggtttaaa tgtattgagt cagaatacag tggcatcgtc
     6061 attaattcgc ttaacacaag agatgccaca tgcacaggca gatgattaca atacgtacca
     6121 tcgttcgcca aggattattc accgtgaacc gacggatgat attaagattg aaagaccgcc
     6181 acagccaata cagaagaaca atacagtgat atggcgttcc attataccgc cattagtaat
     6241 gattgcttta actgttgtca tctttttagt gagaccaatt ggtatttata ttttaatgat
     6301 gattggtatg agtacagtaa cgatagtatt tggtattaca acgtatttct ctgaaaagaa
     6361 aaagtataac aaagatgttg aaaaacgaga gaaagattac aaagcttatt tggataataa
     6421 atctaaagaa attaacaaag cgattaaagc acaacgtttt agtttgaatt accattatcc
     6481 aacggttgct gaaattaaag atatcgttga aacgaaagca ccaagaatat atgaaaaaac
     6541 atcgcatcat cacgatttct tacattataa gttaggtatt gcgaatgtag aaaagtcatt
     6601 caaattagat taccaagaag aagaatttaa ccaacgtcgt gatgaactat tcgacgatgc
     6661 taaagaattg tatgaatttt acacagatgt agaacaagca ccattaatca atgatttaaa
     6721 tcatgggcca attgcatata ttggtgcacg acatctcatt ttagaagaat tggagaaaat
     6781 gctaattcaa ttgtcaacat tccatagtta tcatgattta gagtttctat ttgtgacacg
     6841 tgaagatgaa gttgaaacat tgaaatgggc acgttggttg ccacatatga cattgagagg
     6901 gcaaaacatt agaggatttg tttacaatca acgaacgcgt gaccaaattt taacgtcaat
     6961 ttatagcatg attaaagaac gtatccaagc tgtgcgtgaa cgcagcagaa gtaatgagca
     7021 aattattttc acaccgcaat tagtgtttgt cattacagat atgtcattaa ttattgatca
     7081 tgtcatttta gaatatgtaa accaagattt atcagaatat ggtatttcat taatctttgt
     7141 tgaagatgtg attgaaagtt tgccagagca tgtagatacc attattgata tcaagtctcg
     7201 tactgaaggc gaactgatta cgaaagaaaa agaattagtt caattgaaat ttacacctga
     7261 aaatattgat aacgtcgata aagaatatat cgcgcgacgt ttggcgaatt tgatacacgt
     7321 cgaacatttg aaaaatgcaa ttcctgatag tattacattt ttagagatgt ataacgtgaa
     7381 agaagtagat cagcttgatg tggttaatcg atggagacaa aacgaaacat acaaaacgat
     7441 ggcagtacct ttaggtgtaa gaggtaaaga tgatatttta tcattgaact tacatgaaaa
     7501 agcacacggg ccacatggtt tagttgctgg taccactggt tcagggaaat ctgagattat
     7561 ccaatcatac attttatctt tagctattaa ttttcaccct catgaagttg cattcctatt
     7621 gattgactat aaaggtgggg gtatggcgaa cttatttaaa gatttagtcc atttagttgg
     7681 tacgattaca aacttagatg gcgatgaagc gatgcgtgcc ttaacatcaa tcaaagccga
     7741 attgagaaaa cgtcaacgtt tattcggaga gcatgatgtt aaccatatta atcaatacca
     7801 taagttattt aaagaaggta ttgcgacaga accaatgcca catttattca ttatttccga
     7861 tgagtttgcc gaattaaaat cagaacaacc tgattttatg aaagaacttg tatcaacggc
     7921 acgtattgga cgttcgttag gtattcattt aatacttgcg acacaaaaac catcgggtgt
     7981 tgttgatgac caaatttggt ctaactctaa atttaagttg gcattaaaag tacaagatag
     8041 acaagacagt aatgaaattt taaaaacacc agatgcagca gacattacat taccaggtcg
     8101 tgcgtattta caagttggta ataatgaaat ttatgaatta ttccaatctg catggagtgg
     8161 tgcaacatat gacatcgaag gcgataaatt agaagttgaa gataagacga tttacatgat
     8221 taatgactat ggtcaacttc aagcaatcaa caaagacttg agtggacttg aagatgaaga
     8281 aacgaaagaa aatcaaactg agttagaagc ggtcatagat catatcgaat ctattacaac
     8341 acgattagaa atcgaagaag ttaagcgtcc atggctacca ccattgccag aaaatgtata
     8401 tcaagaagat ttagtagaaa cagatttcag aaaattatgg tcagatgatg caaaagaagt
     8461 ggaattaaca ttaggactta aagacgtacc agaagaacaa tatcaaggac cgatggtatt
     8521 gcaattgaaa aaagctgggc acatcgcgtt aatcggaagt ccaggatatg gtagaacaac
     8581 gttcttacac aacattattt tcgatgttgc aagacaccat cgtcctgatc aagcacacat
     8641 gtacttgttc gatttcggta ccaatggttt gatgccagtt acagacatac cacatgtcgc
     8701 tgattacttt acagtagatc aagaagacaa gattgctaag gcgatacgta tatttaatga
     8761 tgaaattgat cgtcgtaaga agattttaag tcagtatcgt gtcactagta tttctgaata
     8821 tcgaaaatta actggtgaaa caattccgca tgtctttatt cttattgata actttgacgc
     8881 agtaaaagat tcacctttcc aagaagtttt tgaaaatatg atgattaaaa tgacgcgtga
     8941 agggctagca ttagacatgc aagtaacctt aactgcttca agagctaacg ctatgaaaac
     9001 accaatgtac attaatatga aaacgcgtat cgcaatgttt ttatatgata aatcagaggt
     9061 gtcgaacgta gtaggacagc aaaaatttgc ggttaaagat gttgtgggtc gagcattgtt
     9121 aagtagtgat gacaacgtat cattccatat tggccaacca tttaaacatg atgagaccaa
     9181 atcatataat gatcaaatta atgatgaagt atcggcgatg acagaatttt ataaaggtga
     9241 aacaccaaat gatattccta tgatgccaga tgaaattaaa tatgaagatt acagagaatc
     9301 attaaactta ccagatatag ttgcaaatgg tgctttacca attggattag attatgaagg
     9361 tgttacacta caaaaaatta aattaactga accagcaatg atttcatcag aaaatccgag
     9421 agaaattgcg catattgctg aaattatgat gaaagaaatt gacatattaa atgaaaaata
     9481 tgcgatttgt atcgcagact caagtggaga gtttaaagct tataggcatc aagtggctaa
     9541 ctttgccgaa gaaagagaag acattaaagc gattcatcaa ctaatgattg aagacttaaa
     9601 gcaaagagaa atggacggcc catttgaaaa agattcactt tatattatca atgattttaa
     9661 aacatttatt gattgcacgt atattccgga agatgatgtt aaaaagctta ttacaaaagg
     9721 accagaactt ggcttgaaca ttttatttgt cggcattcat aaagaattaa tagatgctta
     9781 tgataaacag attgatgttg cacgtaaaat gattaaccaa tttagtatag gtattcgtat
     9841 ttcagaccaa caattcttta aatttagatt tattcaacga gaacctgtta ttaaagaaaa
     9901 tgaagcatat atggtcgcaa accaagctta tcaaaagatt agatggttta aatagcaatg
     9961 aattaaatag gagggaggta tgttatgaat tttaatgata ttgaaacaat ggttaagtcg
    10021 aaatttaaag atattaaaaa gcatgctgaa gagattgcgc atgaaattga agttcgttct
    10081 ggatatttaa gaaaagctga acaatataag cgattagaat ttaatttgag ttttgcacta
    10141 gatgatattg aaagcacagc aaaggacgta caaactgcaa aatctagtgc taataaggac
    10201 agtgtaactg ttaagggaaa ggcgcccaat acgttatata ttgaaaaaag aaatttgatg
    10261 aaacaaaagc ttgaaatgtt gggtgaagat atcgataaaa ataaagaatc cctccaaaaa
    10321 gctaaggaaa ttgctggcga aaaggcaagt gaatatttta ataaagcaat gaattaatat
    10381 tgaggtgaag atatgggtgg atataaaggt attaaagcag atggtggcaa ggttgatcaa
    10441 gcgaaacaat tagcggcaaa aacagctaaa gatattgaag catgtcaaaa gcaaacgcaa
    10501 cagctcgctg agtatatcga aggtagtgat tgggaaggac agttcgccaa taaggtgaaa
    10561 gatgtgttac tcattatggc aaagtttcaa gaagaattag tacaaccgat ggctgaccat
    10621 caaaaagcaa ttgataactt aagtcaaaat ctagcgaaat acgatacatt atcaattaag
    10681 caagggcttg atagggtgaa cccatgatga aagatgttaa gcgaatagat tatttttctt
    10741 acgaagaatt aacaatttta ggtggtagta aattgcctct cgtaaatttt gaattgtttg
    10801 atccatcaaa ttttgaagaa gctaaagctg ctttaattga aaaggaatta gtaacagaga
    10861 atgacaagtt aactgatgca ggttttaaag tggctacatt agtcagagag tatattagcg
    10921 ccattgtaaa tattcgaatt aatgatatgt attttgcacc atttagctat gaaaaagatg
    10981 aatatatttt gttaagccgg tttaaaaata atgggtttca aatacgaatt atcaataaag
    11041 acattgcatg gtggtcgatt gtacaatcat atcctttatt gatgagacaa gaaaagtcca
    11101 atgattggga ctttaaacaa attgacgatg aaacattgga gaacttaaat aatgaaagta
    11161 tcgatacgat tgggcgtgtt ttagaaattg aaatatacaa tcatcaaggt gaccctcaac
    11221 aaagtttata taacatttat gaacaaaatg atttgttatt cattcgatac ccattaaaag
    11281 ataaagtgct gaatgttcat attggtgtca ttaatacatt tatacgagaa ttatttggat
    11341 tcgatactga tgaaaatcat attaataaag cagaggagta atgacgttga gtggaaaaat
    11401 tagtgttaaa gctgaaacga ttgcacatgt tgtaaaagaa ttggaaagca taagtcaaaa
    11461 gtatgatgaa atagctcaaa actttggaaa aatagcgcaa ttaaattact acagtagtga
    11521 aaaagctgca cattctatgg aaaatggcta tagtagtgct gcaacagtca ttagtggtct
    11581 caaaggtcca ttgagtacac tcggtggtgg cgtcatgaat tcagcacaaa agttctttga
    11641 agcagatgaa cattggggta cggaatttgc caagctttac tataatattg agggataggt
    11701 gcatgacatg acaaaagata ttgaatatct aacagctgat tatgacaatg aaaagtcatc
    11761 tatccaaagt gtaatagatg caatagaggg tcaagacttc ttagatgtag atacaacaat
    11821 ggatgatgcg gtaagcgatg tcagttcttt agacgaagat ggcgcaatat cattaacaag
    11881 tagtgtagta ggtccacaag gatctaaatt aatggggtat tatcaaaatg agttatatga
    11941 ttatgcatct caattagatt cgaaaatgaa agaaattatt gacacgccat ttatagaaga
    12001 tatagataaa gcattcaaag gtataacgaa tgttaaattg gaaaatatac taattaaaaa
    12061 tggtggtgga catggtagag atacctatgg ggcttctggg aaaattgcaa agggagatgc
    12121 caagaaaagt gacagcgatg tttatagcat cgatgaaata ttaaaatcgg atcaagaatt
    12181 tgtaaaagta attgatcagc attacaaaga aatgaaaaaa gaagataaga aattatctaa
    12241 aagtgatttt gaaaaaatga tgactcaggg cgcttcttgt gattacatga cagtagctga
    12301 agcggaagag ctagaggagc aaaagaaaaa agaagaagct atagagattg cagcactagc
    12361 tggtatggta gttttatctt gtattaatcc tgttgctgga gcagtagcta ttggtgctta
    12421 ttccgcttat tcagcagcaa atgcagccac aggaaaaaat attgtaactg gaagaaagct
    12481 atctaaagaa gaacgaatca tggaaggact ttcgcttatt ccattgccag gtatgggctt
    12541 cctcaaaggt gctgggaaaa gtttaatgaa attaggcttc aaaggcggag aaaaatttgc
    12601 agttaaaaca ggattgcaaa agacaatgca acaagcagtt agtcgtattt cacctaaaat
    12661 gggaatgatg aaaaacagtg tgttgaatca atctcgtaac tttgctcaaa atactcatgt
    12721 tggacaaatg ctgagtaaca tgcgtggtca agcaactcat actgttcaac aaagtagaaa
    12781 ttggattgga caacaagcac aaaatgtcaa acgaatagtg aataatggac ttgataaaga
    12841 aatagcacat ccatttaaac aacaacttgc accagcggga atgggtggta taaaatttgc
    12901 tgaaacaact actttgagaa acatgggtca aaacataaag cgtgctgtta caccacaaaa
    12961 tcacgtgaca catggtccaa aagatagtat ggtgagaagt gaaggcaaac atagtataag
    13021 tagccatgaa atgaattcat caaagtatgt tgaatcacca aactacacca aggttgaatt
    13081 cggagaacac tatgcaagac ttagacctaa gaaactaaag gcgaatattg aatacacaac
    13141 acctactggt cacatatatc gaaccgatca taaaggtcgc ataaaagaag tttatgtaga
    13201 caatctctct ctaaaagatg gggatcgtaa tagccatgca caaagaactg tggggggaga
    13261 ggatagatta ccagacgatg atggaggtca cttaatcgct agaatgtttg gtggttcaaa
    13321 agacattgat aaccttgtgg cacaaagtaa atttatcaac cgtccattta aggagaaagg
    13381 tcattggtat aatcttgaga aagagtggca agagttctta aactctggga aagaggtgaa
    13441 aaatattaaa atggaagtaa aatatagcgg taatagtcaa agaccgacta tatttaaagt
    13501 tgaatatgaa attaatggtg aaagaaatat tagaagaata ttaaataagt agaggtgcca
    13561 acatgacatt tgaagagaag cttagcaaaa tatacaatga aattgcgaat gagattagca
    13621 gtatgatacc ggtagagtgg gaaaaagtat atacaatggc ttatatagat gatggaggag
    13681 gtgaagtatt ctttaattat actaaa
//
LOCUS       DAKTIO010000060        13688 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.060, whole genome shotgun sequence.
ACCESSION   DAKTIO010000060 DAKTIO010000000
VERSION     DAKTIO010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13688
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.060"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..588
                     /locus_tag="O0293_001328"
     CDS             <1..588
                     /locus_tag="O0293_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0280217.1"
                     /translation="IVYSTDKDIKKYQKVAPTVVVDYNKHKYLEQQEMLGKIVGKEDK
                     VKAWKKDWEETTAKDGKEIKKAIGQDATVSLFDEFDKKLYTYGDNWGRGGEVLYQAFG
                     LKMQPEQQKLTAKAGWAEVKQEEIEKYAGDYIVSTSEGKPTPGYESTNMWKNLKATKE
                     GHIVKVDAGTYWYNDPYTLDFMRKDLKEKLIKAAK"
     gene            987..1928
                     /locus_tag="O0293_001329"
     CDS             987..1928
                     /locus_tag="O0293_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCY0280218.1"
                     /translation="MTKKVYFNHDGGVDDLVSLFLLLQMENVQLIGVSTIGADCYLEP
                     SLSASVKIINRFSNEDIQVAPSYERGKNPFPKEWRMHAFFMDALPILNEPVKHVASNV
                     SDKEAFEDIIQTLKRQSEKVTLLFTGPLTDLAKALQKDSSIVQYIEKLVWMGGTFLPK
                     GNVEEPEHDGSAEWNAYWDPEAVKIVFDSDIEIDMVALESTNQVPLTLDVRQRWANER
                     QYTGIDFLGVSYAAVPPLTHFITNSTYFLWDVLTTAYIGNKDLVHSIEKKVDVISYGP
                     SQGKTFECKDGRKINVINHVDNNAFFDYITALAKKVN"
     gene            2049..2603
                     /locus_tag="O0293_001330"
     CDS             2049..2603
                     /locus_tag="O0293_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HCY0280219.1"
                     /translation="MTTKQLVYTALMTAIIAILGLVPVIPLPFSSVPIVLQNIGIFLA
                     GVILGRKYGTLSVIVFLLLVVAGLPLLSGGRGGIGVFAGPSAGFLLLYPVVAFMIGAI
                     RDRFINEINFWILFVGILVFGVIALDVIGTLIMGMIINIPFTKAISISLAYLPGDILK
                     AIVASLIGTALLNHSQFRQIMGIK"
     gene            2675..3445
                     /locus_tag="O0293_001331"
     CDS             2675..3445
                     /locus_tag="O0293_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0280220.1"
                     /translation="MASISMSDIYCNGTIFENDDEQLIYLTPSFPQRYTSNTWIYKKT
                     PTQERWLKDLERQHQLHTNQGSNHYAFSFPENEQLDNHWMAMFKDMNFELGIMELYAI
                     ESDALANLPRNSDVEIAIVDESHIDAYLKVAYQFSLPFGKDYADAHEEMVREHYQKDV
                     IKRLVAYLNNEPIGVVDVIESENYIELDGFGVLEQFRHQGIGSTIQSLIGEYAISKNH
                     KPIILVADGEDTAKDMYAKQGYVYQSFCYQILKEDIGN"
     gene            complement(3617..4414)
                     /gene="fdhD"
                     /locus_tag="O0293_001332"
     CDS             complement(3617..4414)
                     /gene="fdhD"
                     /locus_tag="O0293_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCY0280221.1"
                     /translation="MNKDVSLGQPIVRYEDGKLFNTTDQYVTEFPLTIMVNGEEFATV
                     ICSPTNLEELVIGFLASEGAILKRDELKSVLIDDSKGFAHVELNKDLGDRFQYSTKRM
                     IASCCGKSREFYFQNDAAIAKTSMSKITLTPMQIINMMTRLQSASHIYQETGGLHNAA
                     ISDGLTFFVHRQDIGRHNALDKLYGFCIQRHITVRDKVLIFSGRISSEILIKAAKIGV
                     GVILSKSAPTTLAVTLANDLNITAVGFIRNGGFNIYSHPERIIDSEQ"
     gene            4704..5486
                     /gene="modA"
                     /locus_tag="O0293_001333"
     CDS             4704..5486
                     /gene="modA"
                     /locus_tag="O0293_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCY0280222.1"
                     /translation="MKLKRLFAVVIAMLLVLAGCSNSNDNNESKKDDADNGKKQEIQV
                     AAAASLTDVTKKLASEFKKEHKNADIKFNYGGSGALRKQIESGAPVDVFMSANTKDVD
                     ALKDKNKAHDTYKYAKNSLVLIGDKDSNYTSVKDLKDNDKLALGEVKTVPAGKYAKQY
                     LDNNNLFKEVESKIVYAKDVKQVLNYVEKGNAKQGFVYKTDLYKQNKKIDTVKVIKEV
                     ELKKPITYEAGATSDSKLAKEWMEFLKSDKAKEILKEYHFAA"
     gene            5500..6171
                     /gene="modB"
                     /locus_tag="O0293_001334"
     CDS             5500..6171
                     /gene="modB"
                     /locus_tag="O0293_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCY0280223.1"
                     /translation="MPDLTPFWISIRVAVISTIIVTVLGIFISKWLYRRKGSWVKLLE
                     SLLILPIVLPPTVLGFILLIIFSPRGPIGQFFANVLHLPVVFTLTGAVIASVIVSFPL
                     MYQHTVQGFRGIDTKMINTARTMGASETKIFLKLILPLAKRSILAGIMMSFARALGEF
                     GATLMVAGYIPNKTNTLPLEIYFLVEQGRENEAWLWVLVLVAFSIAVISTINLLNKDK
                     YKEVD"
     gene            6172..6777
                     /locus_tag="O0293_001335"
     CDS             6172..6777
                     /locus_tag="O0293_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0280224.1"
                     /translation="MLKINVKYQLKNTLIRINIDDTEPKIYAVRGPSGIGKTTVLNMI
                     AGLRKADEAIIEVNGQLLTDTAKNVNVKIQQRRIGYLFQDYQLFPNMTVYKNITFMAE
                     PSEHIDQLIQTLNIDHLMKQYPMTLSGGEAQRVALARALSTKPDLILLDEPFSSLDDT
                     TKDESITLVKRIFNEWQIPIIFVTHSNYEAEQMAHEIITIG"
     gene            6947..7951
                     /locus_tag="O0293_001336"
     CDS             6947..7951
                     /locus_tag="O0293_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HCY0280225.1"
                     /translation="MSQERYSRQILFKQIGEIGQSKINQKCALIIGMGALGTHVAEGL
                     VRAGIAKLIIVDRDYIEFSNLQRQTLFTEEDALKMMPKVVAAKKHLLALRSDVDIDDY
                     IAHVDYYFLETHGQDVDVIIDATDNFETRQLINDFAYKYRIPWIYGGVVQSTYTEAAF
                     IPGKTPCFNCLVPQLPALNLTCDTVGVIQPAVTMATSLQLRDAMKVLTEQPIDTKITY
                     GDIWEGSHYSFGFSKMQRSDCTTCGDVPSYPYLNKNEQRYATLCGRDTVQYENASITH
                     DILVQFLKQHQLNYRSNSYMVMFEFKGHRIVAFKGGRFLIHGMTRTSDATHLMNLLFG
                     "
     gene            7982..8488
                     /locus_tag="O0293_001337"
     CDS             7982..8488
                     /locus_tag="O0293_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaB"
                     /protein_id="HCY0280226.1"
                     /translation="MGEHQNVKLNRTVKAAVLTVSDTRDFDTDKGGQCVRQLLQADDV
                     EVSDAHYTIVKDEKVAITTQVKKWLEEDIDVIITTGGTGIAQRDVTIEAVKPLLTKEI
                     DGFGELFRYLSYVEDVGTRALLSRAVAGTVNNKLIFSIPGSTGAVKLALEKLIKPELN
                     HLIHELTK"
     gene            complement(8490..8984)
                     /gene="moaC"
                     /locus_tag="O0293_001338"
     CDS             complement(8490..8984)
                     /gene="moaC"
                     /locus_tag="O0293_001338"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCY0280227.1"
                     /translation="MTEFTHINQQGHAKMVDVSDKQITKRTAVAHSSITVNETIFKQI
                     SNNTNTKGNVLNTAQIAGIMAAKNTSTLIPMCHPLPLTGIDVHFSWDETNAPLYTLNI
                     QTTVSTTGKTGVEMEALTAASATALTIYDMTKAVDKGMIIGETYLESKSGGKSGDFQR
                     QSNQ"
     gene            9047..10306
                     /locus_tag="O0293_001339"
     CDS             9047..10306
                     /locus_tag="O0293_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCY0280228.1"
                     /translation="MVVEKRNPIPVKEAIQRIVNQQSSMPAITVALEKSLNHILAEDI
                     VATYDIPRFDKSPYDGFAIRSVDSQGASGQNRIEFKVIDHIGAGSVSDKLVGDHEAVR
                     IMTGAQIPNGADAVVMFEQTIELEDTFTIRKPFSKNENISLKGEETKTGDVVLKKGQV
                     INPGAIAVLATYGYAEVKVIKQPSVAVIATGSELLDVNDVLEDGKIRNSNGPMIRALA
                     EKLGLEVGIYKTQKDDLDSGIQVVKEAMEKHDIVITTGGVSVGDFDYLPEIYKAVKAE
                     VLFNKVAMRPGSVTTVAFVDGKYLFGLSGNPSACFTGFELFVKPAVKHMCGALEVFPQ
                     IIKATLMEDFTKANPFTRFIRAKATLTSAGATVVPSGFNKSGAVVAIAHANCMVMLPG
                     GSRGFKAGHTVDIILTESDAAEEELLL"
     gene            10303..10788
                     /gene="mobB"
                     /locus_tag="O0293_001340"
     CDS             10303..10788
                     /gene="mobB"
                     /locus_tag="O0293_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCY0280229.1"
                     /translation="MILQIVGYKKSGKTTLMRHIVSFLKSHGYTVATIKHHGHGKEDI
                     QLQDSDVDHMKHFEAGADQSIVQGFQYQQTVTRVDNQNLTQIIEKSVTIDTNIVLVEG
                     FKNADFEKVVVYRNEEELQVLQQLSNVCYSINVREHEDFTAFEQWLLNKIKNDCDTQL
                     T"
     gene            10802..11248
                     /locus_tag="O0293_001341"
     CDS             10802..11248
                     /locus_tag="O0293_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HCY0280230.1"
                     /translation="MKQFEIVTEPIQTEQYREFTINEYQGAVVVFTGHVREWTKGVKT
                     EYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPLQISDIAVLIAVSSPHRK
                     DAYRANEYAIERIKEIVPIWKKEIWEDGSKWQGHQKGNYEEAKREE"
     gene            11254..11487
                     /gene="moaD"
                     /locus_tag="O0293_001342"
     CDS             11254..11487
                     /gene="moaD"
                     /locus_tag="O0293_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HCY0280231.1"
                     /translation="MKVLYFAEIKDILQKAQEDIVLEQALTVQQFEDLLFERYPQINN
                     KKFQVAVNEEFVQKSDFIQPNDTVALIPPVSGG"
     gene            11494..12093
                     /gene="mobA"
                     /locus_tag="O0293_001343"
     CDS             11494..12093
                     /gene="mobA"
                     /locus_tag="O0293_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCY0280232.1"
                     /translation="MKAIILAGGHSVRFGKPKAFAEVNGETFYSRVIKTLESTNMFNE
                     IIISTNAQLATQFKYPNVVIDDENHNDKGPLAGIYTIMKQHPEEELFFVVSVDTPMIT
                     GKAVSTLYQFLVSHLIENHLDVAAFKEDGRFIPTIAFYSPNALGAITKALHSDNYSFK
                     NVYHELSTDYLDVRDVDAPSYWYKNINYQHDLDALIQKL"
     gene            12113..13135
                     /gene="moaA"
                     /locus_tag="O0293_001344"
     CDS             12113..13135
                     /gene="moaA"
                     /locus_tag="O0293_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCY0280233.1"
                     /translation="MVEQIKDKLGRPIRDLRLSVTDRCNFRCDYCMPKEVFGDDFVFL
                     PKNELLTFDEMARIAKVYAELGVKKIRITGGEPLMRRDLDVLIAKLNQIDGIEDIGLT
                     TNGLLLKKHGQKLYDAGLRRINVSLDAIDDTLFQSINNRNIKATTILEQIDYATSIGL
                     NVKVNVVIQKGINDDQIIPMLEYFKDKHIEIRFIEFMDVGNDNGWDFSKVVTKDEMLT
                     MIEQHFEIDPVEPKYFGEVAKYYRHKDNGVQFGLITSVSQSFCSTCTRARLSSDGKFY
                     GCLFATVDGFNVKAFIRSGVTDEELKEQFKALWQIRDDRYSDERTAQTVANRQRKKIN
                     MNYIGG"
     gene            complement(13530..13688)
                     /locus_tag="O0293_001345"
     CDS             complement(13530..13688)
                     /locus_tag="O0293_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280234.1"
                     /translation="MSFGQLVVYSTTFIPKKDILQLNGMECDINVKEPLKIYYVVILK
                     SGIEMIFS"
BASE COUNT         4827 a         1910 c         2681 g         4270 t
ORIGIN      
        1 attgtatact ctactgacaa agatattaaa aaatatcaaa aagtagcacc aacagtagtt
       61 gttgactata ataagcataa atatttagaa caacaagaaa tgttagggaa aattgttggt
      121 aaagaagata aagtaaaagc ttggaagaaa gattgggaag aaacaactgc taaagacggt
      181 aaagaaatta aaaaagcaat tggacaagat gcaacagtgt cattgtttga tgaatttgat
      241 aaaaaattat acacttacgg cgataactgg ggtcgtggtg gagaagtatt atatcaagca
      301 tttggtttga aaatgcaacc agaacaacaa aagttaactg caaaagcagg ttgggctgaa
      361 gtgaaacaag aagaaattga aaaatatgct ggtgattaca ttgtgagtac aagtgaaggt
      421 aaacctacac caggatacga atcaacaaac atgtggaaga atttgaaagc tactaaagaa
      481 ggacatattg ttaaagttga tgctggtaca tactggtaca acgatcctta tacattagat
      541 ttcatgcgta aagatttaaa agaaaaatta attaaagctg caaaataatt cagctatata
      601 agttagtgaa atgagagtct gaaacatatc aatcttttga tattgtatta ggctcttatt
      661 tttatagcta gaaagttaga tatttgtatt tttttaaata ataagtgccg ttgttatcgt
      721 tcaatttaat taatgataga ttagtattat tatagctaaa gtagtatacc tgagaaaata
      781 gctcaatgta tctctttatt aataagttat atcataatta ttttagtgca tactttatgg
      841 aagggatatc agggaatggc tttcaattaa agaagaggtt taaaaggatt acaacagaat
      901 gttatgattt tgtagaaaga tatataacaa cgttttataa aaacataata ttgttaatgg
      961 aaaatgaaat gtaaggggga tttcgagtga ctaagaaagt ttattttaac cacgatggtg
     1021 gtgtagatga tttagtatct ctatttttat tattacaaat ggaaaacgtt caattgatag
     1081 gggtcagtac aattggtgct gattgttatt tagagccatc tttgagcgca tcagtaaaaa
     1141 ttattaatcg tttttcaaat gaagatattc aagttgcgcc atcatatgaa cgaggaaaaa
     1201 atccatttcc taaagaatgg cgtatgcatg ccttttttat ggacgcattg ccaattttaa
     1261 atgagccagt caaacatgtt gcttcaaatg tgagcgacaa agaagccttt gaagacatta
     1321 ttcaaacttt aaagagacaa tcagaaaaag taacattatt atttacaggc ccgcttacag
     1381 atttagcaaa agcactacaa aaagattcat ctatcgttca gtatatagaa aaattagttt
     1441 ggatgggtgg caccttttta ccaaaaggaa atgttgaaga acctgagcat gatggttctg
     1501 cagaatggaa tgcatattgg gatccagaag cggttaaaat tgtttttgat agcgatatag
     1561 agattgatat ggttgcttta gaaagtacga atcaagtacc gctaacgtta gatgttagac
     1621 aaagatgggc gaatgaacgc caatatacgg gcattgattt tttaggagta agttatgcag
     1681 cggtaccacc attaacacac tttataacaa attctactta ctttttatgg gatgttttaa
     1741 cgactgctta tattggtaac aaggacttgg ttcattcaat tgagaaaaaa gtcgatgtaa
     1801 taagttatgg accaagtcaa ggtaagacat ttgagtgtaa agatgggcgc aaaattaatg
     1861 tcataaatca tgtagataac aacgcatttt ttgattatat aactgcactt gctaaaaaag
     1921 taaattaaca gctgtgtaga ataattaagg ttttaattta tatagaacaa cttattgtaa
     1981 acttttcatt tcttaaagtt tacaatggtg ctataataat ggtcatgaaa tacgaaagga
     2041 agtaaaaaat gacaacaaaa cagttagtat atacagcttt aatgacagcg attatcgcta
     2101 ttttaggatt ggtaccggta attccactac cattttcttc agtaccaatt gtacttcaaa
     2161 acattggtat tttcttagca ggtgtgattt taggacgtaa atatggcaca ttaagtgtta
     2221 tcgtcttttt attattagta gttgctggct tgccattgtt atcaggtggt cgcggtggca
     2281 tcggtgtatt cgcaggtcct tcagcagggt ttttactatt atatccagtt gtagcattca
     2341 tgattggggc gattcgagat agattcatca atgaaattaa tttctggatt ttattcgttg
     2401 gtattttagt ttttggtgtt atagcattag atgttattgg tacattgatt atgggcatga
     2461 ttattaacat accatttacg aaagctattt caatttcatt agcttatttg cctggtgata
     2521 tattaaaagc aattgtagca agtttgattg gtacagcttt acttaatcac tcgcagtttc
     2581 gtcaaattat gggaataaaa taatcatatt taagatagta aagtaattga ataagttgct
     2641 ttgaaattta taaaagtgaa aggagtaggt gtcaatggct agtataagta tgtcagatat
     2701 atattgtaac ggcactatat ttgaaaatga cgacgagcag ttgatttatt taacgccttc
     2761 ttttccacaa cgatacacaa gtaacacatg gatatataaa aagacgccta cccaagagcg
     2821 atggctgaaa gacttagaac gtcaacatca attacataca aatcaaggtt caaatcatta
     2881 tgcgtttagt ttcccggaaa atgaacaact tgataatcat tggatggcta tgtttaaaga
     2941 tatgaatttt gaactaggta ttatggaatt gtatgccata gaaagtgatg cgcttgccaa
     3001 tttgccgcgt aactctgacg ttgaaattgc catcgttgac gagtcgcata tagatgccta
     3061 tttaaaagtt gcatatcagt ttagtttgcc atttggaaaa gactatgcag atgcacatga
     3121 agaaatggta agggaacatt atcaaaaaga tgtgattaaa cgcttagtag cttatttaaa
     3181 taatgaacct attggcgttg tagatgtcat tgaaagtgaa aattacattg aattagatgg
     3241 atttggtgta ttagaacaat ttcggcacca aggaattgga tctacaattc aatcgttgat
     3301 aggtgaatac gccatatcaa aaaatcacaa accaatcata ttagttgcag atggtgaaga
     3361 tacagcaaaa gatatgtatg caaagcaagg ttatgtctat caatcgtttt gttatcaaat
     3421 attaaaagaa gatattggaa attaacgatt gatgataggt ttgtattaga ataaaaaagc
     3481 agtaagctgg tttcgagtag aaatcaactt actgcttttt aaattgtttt gagctactta
     3541 tacttataaa aatagtgcgt ttaaattgtt gattcatgta gaatatcgtt cattatgaca
     3601 cactataatg aatatgttat tgttcagaat caatgatacg ttctggatga ctgtatatat
     3661 taaagccacc atttcgaata aatccaactg ccgtaatatt taggtcatta gctaaggtta
     3721 cagcaagcgt tgtcggagct gatttagata aaatgacgcc aacaccaatt tttgcggctt
     3781 taattaaaat ttctgatgaa atacgtccac taaaaattaa tactttatct cggacagtaa
     3841 tatgtcgctg aatacaaaat ccatataatt tatctagagc gttatgtcta ccaatgtctt
     3901 gtcgatgtac aaaaaatgtc aaaccatcgc ttatagcagc attatgtaag ccacctgttt
     3961 cttggtaaat atgacttgca ctttgtaatc gagtcatcat gttaataatt tgcattggag
     4021 ttaaagtgat tttagacata gatgttttag cgatagcagc atcattttga aaataaaact
     4081 cacgactctt tccgcaacaa gatgcaatca ttcgttttgt ggaatattga aagcgatcgc
     4141 ctaaatcttt attaagttca acatgggcaa aacctttact atcatcaatc agtacagatt
     4201 ttaattcatc tcgctttaaa atggcacctt ccgaagccag aaatccaatg actaactcct
     4261 caaggtttgt tggactgcat ataacagtcg caaattcttc accattcacc ataattgtaa
     4321 gtggaaattc tgtcacatat tgatctgttg tattgaataa ttttccatct tcatatctaa
     4381 caattggttg acctaaagat acatctttgt tcattatcta acccctttaa ttagcttaaa
     4441 ctttatttta aagcaatttg cttaaaattt taacatattt gcttaagttt gaaatttgat
     4501 tgataaaaat taatagcgag caatctgttt gatttaaatt gaattcgaga atatacatac
     4561 tagggcatca attaataaat atcaatctta tgcaaatttg acaattgttt gaatcaatat
     4621 ataaacaggc aacggttctt ttcaaatata atagtaagtg tataatgaaa atgtaaatat
     4681 tattaaaaat gggggttcac tcaatgaaat tgaaacgttt atttgctgtt gtgattgcaa
     4741 tgcttttagt attagctggt tgctctaatt ctaacgataa taatgaaagt aaaaaagatg
     4801 acgcagacaa tggtaagaaa caagagattc aagttgcagc ggcagcaagt ttaacagatg
     4861 taaccaagaa attagcttca gaatttaaaa aagagcataa aaatgctgat attaaattta
     4921 actatggtgg atcaggggca ttaagaaaac aaattgaatc aggcgcacct gttgacgtat
     4981 ttatgtctgc aaatactaaa gatgtagatg cattaaaaga caagaataaa gcgcatgata
     5041 catataaata tgcgaaaaat agtctagtat taattggtga taaagattca aattacactt
     5101 cagtaaaaga cttaaaagac aatgataaat tagcattagg tgaagtgaaa actgtaccag
     5161 caggaaaata tgcgaaacag tatttagata acaataactt atttaaagaa gtcgaaagta
     5221 aaatcgttta tgctaaagat gtaaaacaag tattaaatta tgttgaaaag ggtaatgcga
     5281 aacaaggttt tgtgtataaa actgacttat ataaacaaaa taaaaaaatt gatactgtaa
     5341 aagtaattaa agaagtagaa cttaagaagc caatcacata cgaagctggt gctacatcag
     5401 atagtaaatt agcaaaagag tggatggaat tcttaaaatc agataaagct aaagaaatac
     5461 taaaagaata ccactttgca gcataaggag ttgtaatcca tgcctgactt aacacctttt
     5521 tggatatcaa tacgagttgc tgtaatcagt acgattattg taacggtttt aggtattttt
     5581 atatctaaat ggttgtatcg tcgtaagggt tcgtgggtta aattattgga aagtttattg
     5641 atattaccta ttgttttgcc gccaacggta ttaggtttta ttctattaat catcttctcg
     5701 ccaagaggac caatcggtca attctttgcg aatgtactac atttacctgt agtgttcact
     5761 ttgacaggtg ctgtgatagc atctgtcatt gttagttttc cactaatgta tcaacatact
     5821 gtgcaaggct tcagaggtat agacacgaaa atgattaata cagctagaac gatgggagca
     5881 agtgaaacga aaattttcct caaattaatt ttaccattag ctaaacgctc tattttagca
     5941 ggtataatga tgagttttgc tcgtgcatta ggtgagtttg gtgctacatt aatggttgca
     6001 ggatatattc caaataaaac gaatacacta cctttagaaa tatacttctt agtggaacaa
     6061 ggtagagaaa atgaagcgtg gttatgggta ttagtgctag tcgcattctc tattgcggtt
     6121 atatctacaa ttaatttatt gaataaagat aaatataagg aggtcgacta gatgcttaaa
     6181 atcaatgtga aatatcaatt aaagaacact ttaattcgca tcaatataga tgatactgaa
     6241 ccaaaaattt atgcagttcg tggtccatct ggcattggta aaactactgt tttaaatatg
     6301 attgccggat tacgtaaagc agatgaagct attatcgaag tgaatgggca attacttact
     6361 gatacggcaa aaaacgtgaa tgttaaaatt caacaacgac gtattggata tctgtttcaa
     6421 gactaccaat tgtttcctaa tatgacggtc tataaaaata ttacttttat ggctgaacca
     6481 tctgaacaca tcgatcaatt aattcaaact ttaaacattg atcatttgat gaaacaatat
     6541 cctatgacat tgtcaggtgg agaggcacaa cgtgtagcac ttgcacgtgc acttagcacg
     6601 aaaccagatt taattttatt agatgaacct ttttctagtt tggatgatac tacaaaagat
     6661 gagagtatta cattagttaa acgtattttc aacgaatggc aaataccaat catatttgtg
     6721 acacattcaa actatgaagc agaacaaatg gctcatgaaa ttattacaat tgggtaatca
     6781 tttatttgcc attaaagagt ttagaacgta tttaaaattg tagaagtgaa tgcttctatc
     6841 agcattttaa tgatgtttta aactcttttt taggggcagt ttttttgaga gacattgacg
     6901 cgcgtcatat aatgaaagta atgataaaaa gaaaggataa cttaatgtga gtcaagaacg
     6961 ttattcaagg caaattttat ttaaacaaat aggtgaaata ggtcaaagca aaataaatca
     7021 aaaatgtgcg ttgattattg gtatgggcgc attaggtaca catgtggccg aaggacttgt
     7081 tagagcaggc attgccaaac taatcattgt tgatagagat tatattgaat ttagtaattt
     7141 acaaagacaa acattgttta ctgaagaaga tgctttgaaa atgatgccta aggtggttgc
     7201 agctaaaaag catttgctag cgttacgtag tgatgttgat attgatgatt atattgccca
     7261 tgtggattat tattttttgg aaacacatgg acaggacgtt gacgttatta ttgatgcaac
     7321 cgataacttt gaaacacgac aactgattaa tgattttgca tataaatatc gtataccttg
     7381 gatttatggt ggtgttgtac agagtacata tacagaagct gcatttatac ctggtaaaac
     7441 accttgcttt aactgtttgg taccacaatt gccagcatta aatttaacat gtgatacagt
     7501 aggggtcatt caacctgccg tgacgatggc aacaagttta caattaagag atgcgatgaa
     7561 agtattaacg gaacaaccaa ttgacacaaa aataacttat ggcgatattt gggaaggtag
     7621 tcattattca tttggtttca gtaaaatgca acgttcagac tgtacaactt gtggagatgt
     7681 accaagttat ccgtatttaa acaagaatga acaacgttat gcaacattgt gtggtagaga
     7741 cactgtacag tatgaaaatg catcaattac acacgacatt cttgttcaat ttttaaaaca
     7801 acatcagtta aattatcgca gtaattcgta tatggttatg tttgaattta aaggacaccg
     7861 cattgttgct tttaaaggtg gaaggttttt aatacatggc atgacacgca catcagatgc
     7921 cacacatcta atgaatttat tgtttggata aaaaaagata agacaaaagg agtgtaatat
     7981 tatgggcgaa catcaaaacg ttaaattgaa tcgtacagtt aaagcagccg tactaacggt
     8041 atcagatact agagactttg atacagataa aggtggtcaa tgcgtgcgcc aactattaca
     8101 agcagatgac gttgaagtga gtgacgcaca ttatacaatt gtgaaagatg aaaaagtagc
     8161 catcacgacg caggtgaaga agtggttaga agaagatatt gatgtcatca ttacgactgg
     8221 tggaacaggt attgcacaac gtgatgtgac gattgaagca gtaaaaccac ttttaactaa
     8281 agagatagat ggctttgggg aattgtttag atatttgagt tatgttgaag atgttggcac
     8341 gcgtgcatta ttgtctcgtg ctgtagcagg tacagttaat aataaattga tattttcgat
     8401 tccaggatca acaggcgcag ttaaattagc attagaaaag ctcattaaac cagaattaaa
     8461 tcatctgatt catgagctta caaaataatt tattgatttg attggcgttg aaaatctcca
     8521 gatttaccgc cagacttgct ttcaaggtag gtttcgccaa taatcatacc tttatcaact
     8581 gctttcgtca tgtcgtaaat ggttaaagcc gttgctgatg cagcggttaa agcttccatt
     8641 tcaacaccgg ttttgccagt tgtagagaca gttgtttgaa tgtttaaagt ataaaggggt
     8701 gcatttgttt catcccagct gaagtgaaca tctatgccag tcaatggtaa tggatggcac
     8761 atcggaataa gtgttgatgt atttttggca gccataatac cagcgatttg agcagtgttc
     8821 aatacattgc ctttggtatt ggtgttatta gaaatctgct taaaaatagt ttcatttaca
     8881 gtaatgcttg aatgagcgac agcagttctt tttgtaattt gtttgtctga tacatcgacc
     8941 attttggcgt ggccttgttg attaatatga gtaaactcag tcattttacc cctcctagtg
     9001 catctagtat atcatgaaaa aataaaagtt ttggagatga tttttaatgg tagtagaaaa
     9061 aagaaaccca atcccagtta aagaagcaat tcaacgtatc gttaatcagc agagttcaat
     9121 gccggcaatt acggtagcac ttgaaaaaag tctaaatcat atcttagcag aagatattgt
     9181 agctacttat gatataccaa ggtttgataa atcaccttat gatggttttg caattcgcag
     9241 tgttgattca caaggggcaa gtggtcagaa tcgcattgag tttaaagtga ttgatcatat
     9301 tggtgcaggt tcagtttctg ataaattagt tggggatcac gaagcggtgc gtattatgac
     9361 tggagcacaa atacctaatg gcgcagatgc tgttgttatg tttgaacaaa cgattgaact
     9421 agaagataca tttacaattc gtaaaccatt ttcaaaaaat gaaaatatat ctttaaaagg
     9481 tgaagaaaca aagacaggcg atgttgttct aaaaaaagga caagtaatta atccaggggc
     9541 tatcgcggtc cttgcaacat atggctatgc agaggttaaa gttattaagc aaccgagtgt
     9601 cgctgttatt gcaacaggaa gcgaattatt agatgttaat gatgtattag aagatggaaa
     9661 aattcgtaac tctaatggcc caatgattcg tgccttagca gaaaaattag gtcttgaagt
     9721 tggtatttac aaaacacaaa aagatgattt agatagtggc atccaagtcg ttaaagaagc
     9781 tatggaaaaa catgatatcg ttattacaac gggcggagtt tctgttggag attttgacta
     9841 tttacctgag atttataagg ctgtaaaggc ggaagtgtta tttaataaag tagcaatgcg
     9901 tcctggtagc gtaacaacgg ttgcatttgt agatggaaag tatttgtttg gattatctgg
     9961 aaatccatca gcttgtttta caggatttga actatttgtg aaaccagctg ttaaacatat
    10021 gtgtggcgca ctagaagtct tcccgcaaat aattaaagca acattaatgg aagattttac
    10081 caaggcaaac ccattcacac gatttatacg tgctaaagca acgttaacaa gtgctggagc
    10141 tactgtagta ccttcaggat tcaataaatc aggtgcggtt gtagcgattg cacatgctaa
    10201 ctgtatggtc atgttaccag gagggtcacg tggttttaaa gcggggcata cagtagatat
    10261 tatattgact gaatctgacg ctgctgaaga ggaacttctt ttatgatttt acaaattgta
    10321 ggttacaaaa agtctggtaa gacaacattg atgaggcata ttgtctcttt cttaaagtca
    10381 catggttata cagttgctac tattaaacat catgggcatg gtaaggaaga tattcaatta
    10441 caggattcag acgtcgatca catgaagcat tttgaagcgg gggcagatca aagtattgta
    10501 caaggttttc aatatcagca aactgtaaca cgtgtagata atcaaaatct tactcaaatt
    10561 attgaaaaat ctgttacaat tgacaccaat atcgtattag ttgaaggctt taaaaatgct
    10621 gattttgaaa aagtcgtagt ctatcgaaat gaagaagagt tgcaagtatt acaacaattg
    10681 tcgaatgttt gttatagcat taatgtaagg gagcatgaag attttacagc atttgagcaa
    10741 tggttattaa ataaaattaa aaatgattgt gatacacaat taacatagag gattgaaatg
    10801 aatgaaacaa tttgaaatcg tgacagaacc gatacaaaca gaacaatatc gtgaattcac
    10861 tataaatgaa tatcaaggtg cagtagttgt ttttaccggt catgttcgcg aatggactaa
    10921 aggcgtcaaa acggaatatt tagaatatga agcgtatatt ccaatggctg aaaagaaatt
    10981 ggcacaaatt ggagatgaaa taaatgaaaa atggcctgga acgataacga gtattgttca
    11041 tagaataggg ccattacaaa tttcagatat cgctgtatta attgcggttt cttcaccgca
    11101 tcgtaaagat gcctatcgag caaatgaata tgcaattgag cgtataaaag aaattgttcc
    11161 gatttggaaa aaagaaattt gggaagatgg ttcaaaatgg caagggcatc aaaaagggaa
    11221 ttatgaagaa gcgaagaggg aggaataaga gagatgaagg tactttactt cgcagaaatt
    11281 aaagatatat tacaaaaagc acaggaagat attgtgcttg aacaagcatt gactgtacaa
    11341 caatttgaag atttattgtt tgaacgttat ccgcaaatca ataataaaaa gtttcaagtt
    11401 gctgtaaatg aggaatttgt acaaaaatcg gatttcattc aacctaatga tactgttgca
    11461 ttaattccac cggttagtgg aggttaaggg agcatgaaag caataattct tgcaggtggt
    11521 cattcagtgc gatttggtaa gcccaaagct tttgcggaag tgaacggtga gaccttttat
    11581 agtagagtaa ttaagacatt agaatcaaca aatatgttca atgaaattat tattagtaca
    11641 aatgcgcaat tggcaacgca atttaaatat ccaaatgttg ttatagatga tgagaatcat
    11701 aatgataaag gtccattagc aggaatttat acaatcatga agcaacatcc tgaagaagaa
    11761 ttgttttttg tcgtttctgt tgatacacca atgattactg gtaaagctgt aagcacgttg
    11821 tatcagtttt tagtttctca tcttattgaa aatcatttag atgtcgcagc ttttaaagaa
    11881 gatggacgtt ttattccaac aattgcattt tatagtccga atgcattagg cgctataact
    11941 aaagcactac attctgataa ttacagtttt aaaaatgtat atcatgaatt atcaacggat
    12001 tatttggatg taagggatgt agatgcgccc tcatattggt acaaaaatat aaattatcag
    12061 catgatttgg acgctttaat tcaaaaattg taagctgtta ggaggtccac aaatggtaga
    12121 acaaataaaa gataaactag gacgtcccat ccgtgactta cggttatctg tgacagatcg
    12181 gtgtaacttt aggtgtgatt attgcatgcc taaagaggta tttggagatg atttcgtatt
    12241 tttacctaaa aatgaacttt taacgtttga tgaaatggct agaatcgcta aggtatatgc
    12301 agaattaggt gtaaaaaaaa tacgcattac aggtggagaa ccattgatgc gacgggattt
    12361 agatgtactt atagctaaat taaatcaaat cgatggtatt gaagatattg gtttgactac
    12421 aaatggtttg ttattaaaaa agcatggaca aaagttatat gatgctgggc tgcgcagaat
    12481 taatgtcagt ttggatgcta ttgatgatac gctatttcaa tcaatcaata atcgtaatat
    12541 taaagcgact acgattttag aacaaattga ttacgcgacg tctattggtt tgaatgtaaa
    12601 agtaaatgtt gttatacaaa aaggtattaa cgatgatcaa atcataccaa tgcttgaata
    12661 ttttaaagat aaacatatag agattcgatt tatagaattt atggatgttg gtaatgataa
    12721 tggatgggat ttcagtaaag ttgtaactaa agatgaaatg cttacaatga tagagcagca
    12781 ctttgaaatc gatcctgtag aaccaaaata ttttggggaa gtagcaaaat attatcgcca
    12841 taaggataat ggtgttcaat ttggtttgat tacaagtgtt tcacaatcat tttgttctac
    12901 atgtacacgc gcaaggctgt catcagatgg gaagttttac ggatgtttat ttgcaactgt
    12961 cgatggattt aacgttaaag cgtttattcg ttctggcgtg accgacgaag aattaaaaga
    13021 acaatttaaa gctttatggc aaataagaga tgatcgatat tcagatgaga gaactgctca
    13081 aacagttgcc aatcgtcaac gtaaaaagat aaacatgaat tatattggtg gttaatgtgt
    13141 agggaccact acatattaaa tcattagaga tgttttaata tttctgtctt actccctaaa
    13201 atacaatatt atttattaaa gtaaaaacgg tcatatctat gccagattta atagaaatga
    13261 tcgtttttaa agtttttaca agttggcggg gccccaacac agaagctgac agaaagtcag
    13321 cttacaataa tgtgcaagtt ggcggggccc caacatagag aatttcaaaa agaaattcta
    13381 cagacaatgc aagttgggga acggggcccc aacacagaag gtgacgaaaa gtcagcatac
    13441 aataatgtgc aagttggcgg ggccccaaca tagagaattt caaaagaaat tctacagaca
    13501 atgcaagttg gggatcaacg aaataaattt tatgagaata tcatttctat cccactctta
    13561 agaatcacta cataataaat ctttagtggt tctttaacat tgatgtcaca ctccatgcca
    13621 ttgagttgta atatatcttt tttaggtata aatgttgtcg aataaacaac aagttgtcca
    13681 aaagatat
//
LOCUS       DAKTIO010000061        13636 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.061, whole genome shotgun sequence.
ACCESSION   DAKTIO010000061 DAKTIO010000000
VERSION     DAKTIO010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13636
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.061"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(50..238)
                     /locus_tag="O0293_001346"
     CDS             complement(50..238)
                     /locus_tag="O0293_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCY0280235.1"
                     /translation="MNNNEENSVFFGKKKKVSLHLLVDPDMKDEIIKYAQEKDFDNVS
                     QAGREILKKGLEQIKSNK"
     gene            410..1384
                     /locus_tag="O0293_001347"
     CDS             410..1384
                     /locus_tag="O0293_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transfer complex protein TraA"
                     /protein_id="HCY0280236.1"
                     /translation="MDNITFRKRIEEYRKRSIEQKKYESKLNYQSRFGVKDDEVSTFN
                     IKGIATDKKENKLVMAEDREDKQKVLLYANKDNDMYYLNSGSTYKEAVQNTFGNETNY
                     EMVDDELFIYNSKNQALTNYDQLDEYHNRQKQLQYNTKLEQERKQRLTDTNKVSENAK
                     EDLALSLGLTATALKGNINISNVEKVTDNESMVTIENEINGKSEHLISDFKDSFIEKA
                     KEKLTVSPSMRLGFEEFKNASGQIAMGFTEFFGARYDESTLENDPKVVATEKGYSVSV
                     FDKDGNKKDLTSADTFEEAVKLSKEMKHDNSLSRSEIELNQELEKKKI"
     gene            1401..1718
                     /locus_tag="O0293_001348"
     CDS             1401..1718
                     /locus_tag="O0293_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280237.1"
                     /translation="MIKKFSLTTVYVAFLSIVLSNITLGAENPGPKIEQGLQQVQTFL
                     TGLIVAVGICAGVWIVLKKLPGIDDPMVKNEMFRGVGMVLAGVAVGAALVWLVPWVYN
                     LFQ"
     gene            1778..2185
                     /locus_tag="O0293_001349"
     CDS             1778..2185
                     /locus_tag="O0293_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280238.1"
                     /translation="MSVEQEVKDIMLDMLGEEKKVKENIIPRNVDASYKIFLNLNGKE
                     LAIVFVPFLLFLTIAFGVTFLMGLLNLITGFMVFIVGLIFGLTIYGLLTIRPISTKEN
                     IRMIDTIKQSQRFSRRQKVYFYKSKEGLGDDDV"
     gene            2172..2855
                     /locus_tag="O0293_001350"
     CDS             2172..2855
                     /locus_tag="O0293_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traD"
                     /protein_id="HCY0280239.1"
                     /translation="MMMFKKKNKQNKNNEQQFEIINRYIKGDGRNQSIQEDSLIAGFY
                     KDYVVTKDGYISGGLNITGVNTELLTKFEQNDLLEEYSSFISNILYDEPMIKTITEPV
                     NIQNYIKHLYRQVLLDMKNNNNKPTRRGQLIAGRILFLQEKLAKGEMSTKKHYIFFSE
                     KITENTVAQLDHAVNRLHEKINLNKEALQQAFSHYGLETRPIYQTEYLNQFYALYDYK
                     GYNDYHLSN"
     gene            2876..4888
                     /locus_tag="O0293_001351"
     CDS             2876..4888
                     /locus_tag="O0293_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCY0280240.1"
                     /translation="MAFLKKKKQEQVTNKIHNESFQKLTEVLDTDSVDSIYPFSWIEK
                     KSHIETGENYIKNLLVVDYPQSVKGAYLSNLLKKNGNIQITKFIRPANIERMIDHLNN
                     SIKNKTAEQMRTTDPKRNATIKREIESSKKQLDKFLDEKTGFMYMYMYITLNGDSYEK
                     IQALEKDVKRTLTRLRLKTHTPTNAMRESFHTVLPLNRNFLSAFTQQNMDTATAGHFF
                     MFDDSEIIDLTPNTSVFGINKNTDSLVAVDFNNKEKTLNKNMTIIGTSGVGKSTLNMR
                     MILDNIKKSIRQFIIDPEDEFSYITKYYGGTVVNISTSSNIKINPFEIFSEEVFEKED
                     ETDNETTSDVLTENNEHESQIDTLVRSKIGKLKTFFRVLKDEISQTEISVLSSTLRQL
                     YQDKGFKGNAKLSDFKSEDYPTLTELYNKLKDLDPEKYEVLKDLTLIIEDYTMEHGTT
                     TIFDGYTNIKLDNEIVTFNLKPLQTEKDVQSAAYLNIFSFLWDEITKDRTTETVLMTD
                     ELHFLATNEYSLDFYYQAYKRIRKYGGGAIASTQQIKDILRTSQEIGSAIIENSHTKF
                     FFGMDNVGVDDVVDKLGLKFSDQEISHLTKKKKGEALLLYGTQRAFIRIDLDREETRL
                     WNKELYETIYEEPADVEPNYVEQLGLTDIDLAELEEELRQAEMIYE"
     gene            4900..6180
                     /locus_tag="O0293_001352"
     CDS             4900..6180
                     /locus_tag="O0293_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280241.1"
                     /translation="MKIRNFNEGTLVGKHNHLEYIINVDLCHISDIDEIRYDLTEPNE
                     LFFEVKDLQKEGDYFHIIYNTDNEYNSFLSAKKESKALKLSLMDYVLKVDPLKDNITV
                     MHPANLFFKNIEDIKIGFKGHEYLPKPKINNLEQYKLLIMSTLSQYTFDKYYRNKFEV
                     LSKEKDDFFHKVNNAETFEKLKEIVRHELNQVQTDYLLNEEKRKRETRKKYIRNLILG
                     FVGIAIVISLISFMIINGKQQELDSKIAQADKQEQRSKVYENLYNGNVDQAVKGMKRD
                     DSFNKKDIEKTLKKEKKYEELIELSSKNTPYVIEQLYKEGKQNKIRELAFNFENNDTL
                     SLEKKILDKDGTAFSVGSTGKYEEQSKRLALASAKEGYVESAKDINKKLKDNEVEEEI
                     NKAEIKQLKEEKDSTKDKDKKKEIQKDIEELEKK"
     gene            6198..7274
                     /locus_tag="O0293_001353"
     CDS             6198..7274
                     /locus_tag="O0293_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0280242.1"
                     /translation="MWSAIAKGITMLMKKKALSKILKPVIAIVIGFFLLIFIMIAYLV
                     GSFTKEINESGEQKMKEAESTATGACTGGSVDGSGISAFEKNAKGGALEGKGKDMVKI
                     AKKNKIPPKLFIAIVASESEWGRGANATKQKNPLSIMGAGPLQVYPSIEEGLDKGAKN
                     LYDLYISEGLTTPEKIGPKYAPVGASNDPDDLNSNWVPTVKKIMKSFGGKEAKCSTES
                     SGGGSDGKGFDFKGEFPKPDKSKYNGQSYPWGQCTWYVHQRRKEIGKPVPLTWGNGGD
                     WGDNAKAQGWEVGSKPKAGAGASVKPGNFGAPPPYGHIMFVEKVKKDGGIVVSEANVK
                     GLGVISSREFSKAETQRMQFIYDK"
     gene            7284..7769
                     /locus_tag="O0293_001354"
     CDS             7284..7769
                     /locus_tag="O0293_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HCY0280243.1"
                     /translation="MKKLLTIVILFTLVLAGCGQNQSEKNSEKHNEEKTVDTTEERND
                     MIKGLIEYGYENDDYKGYKHMKDILSEKLQKQAELQNQSAQTKGVKKETKDIKLYQDV
                     DNDDELIYSIKLSITNKKEKNSDYSMRYGKIELTEEEGKQKIDNVEELTQRNLVSMNQ
                     E"
     gene            7785..9887
                     /gene="topB"
                     /locus_tag="O0293_001355"
     CDS             7785..9887
                     /gene="topB"
                     /locus_tag="O0293_001355"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001094110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCY0280244.1"
                     /translation="MNTLILCEKPSQAMDLSTVFAKKKKQNGYMEISDEQLNVSGFLT
                     WAVGHLVELKEPQEYDEKYKNFSTYPILLEKDDFQFKVSDKTKDQFNNIKKIFKENKI
                     DEVIIATDPAREGENIAYKILNQLKVTDKVTIKRLWLTSKVESSIRKAFKNILPKEKT
                     YGFYKEGRARELSDWLVGINLSRHFTKISRELGNDGVIHIGRVSSPTLNMVYNRENNI
                     KGFKGKKFYKVSATINKDEQEVKTELKNKFDSEDELHEFLFENDITDLTQKGLVTDIE
                     KEIGYTMPPKFYDLSALQEDMNDKYKISAKRTLEIAQTLYEKKLITYPRTDSRYITED
                     EKEMLLENIDYLKEITKINLNNELTNNSLINPSKIEDHYAILITGNDFNKVDLKEEEI
                     NVYKSILQNVAMNFMDKEQYETTTIEIAVKKLMFEVKGKIIQDNGFKALLNKQKTSEE
                     TIPNFEKNEEVDIELDLLEKETTPPKRYTEKTLLKAMANPIETLEDEGLKSTLKEVKG
                     LGTPATRADIIENLKKNKYIQVQKNKIYITKNGILACLLLEGHLLSKPDLTGQWEKYL
                     NGISKGEKDDDSFINTINEMIKKTINEEVKNKESIQKVAKEKVSTNNIAKCPACDNGY
                     LIDRKGFYGCTEYNNGCEFTIPKKLLEKSIPPTVVKALCESKTTKKLKGFKSKKSGKS
                     FDCKLILTKENKLQFSFD"
     gene            9903..10367
                     /locus_tag="O0293_001356"
     CDS             9903..10367
                     /locus_tag="O0293_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000715274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280245.1"
                     /translation="MKKGIFILTGIIWLVLIYLAQILAYALNTLSNTSNKQKIFSDIS
                     QLFIPPAKHPISTIIGFVSDKNTVFFVIGVIATIICIYILIKQLFESKDGNEKNQDYK
                     IAKHGSHGSAKFAEDSELFSEGHYKKMKENDITNYVYNSLDKSLIEERSDKE"
     gene            10364..12004
                     /locus_tag="O0293_001357"
     CDS             10364..12004
                     /locus_tag="O0293_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HCY0280246.1"
                     /translation="MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSYK
                     SAGYVIPNVIVKNQQSIVVTDPSGEVYEKTSNIKRMQGFDVRVVNFKNFLASDRQNFF
                     DYIDKDSDCSIVAELIIKSAGDSKINKDVWYKASVGLLNSLLLYAKYEFEPEKRTIGN
                     IIKFIQNNKPNQDEEGSVELDKRFNELSKDHPARESYEFGFAVSEGRTRASIILTFVA
                     DLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLY
                     RLGDENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKY
                     NKDKANAIIGNHAVILCLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSS
                     NKNVSYTGRALLNEDEISNMQQDKAILITKNRNPKIIKKLAYFKMFPNLTETYIAPQN
                     EYKRKKNQSMIDKHNRLREEWDKKQEKNKQQKLEEYKEEQRQKELEKEQQPQEEQQNE
                     KVKESKDKNDQEDKKDEQQKINDSKKAFYEKLKQKQAK"
     gene            12082..12999
                     /locus_tag="O0293_001358"
     CDS             12082..12999
                     /locus_tag="O0293_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000383805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280247.1"
                     /translation="MDWIKDAIKELTEGIADGMMNFIFQFLYTLFYEPLKFFIWLLDK
                     ILIDPNSISSDYISNASVLVNSLITSIAICVFAFKMVQVMKQNAEGNAESPGYYVAQL
                     LPSSLLVAVLPWLVDIMTKLSYSATRAFLDVGQKSYLETIKEWSSLKKGQDGFKEMLG
                     SMGTMAGSQIMFILFILLILVFTVVFIFQFVSRIADLVIMKVVAPLVAVSLLADENNY
                     IGVWWRELLAISIQLPLQVFTFFGGINLFFGAKLDVGTLLLGIGFFIITVKSPSFIRS
                     MVYSTGSGRMATGMAGSGAKLLVRKMLSK"
     gene            13016..13408
                     /locus_tag="O0293_001359"
     CDS             13016..13408
                     /locus_tag="O0293_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCY0280248.1"
                     /translation="MINKVIYSGRIATDLKLRKSKNEKSYVFFNIAVPRQFDNDRTDF
                     INCVVWGKQAESLVDYQSKGNLIIIEGSTETYKKDNINMSRCLVEKTIFLESKKENKK
                     HDVSEDDAISPAEDSGNDKSISDDDLPF"
     gene            13465..13632
                     /locus_tag="O0293_001360"
     CDS             13465..13632
                     /locus_tag="O0293_001360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280249.1"
                     /translation="MKVTNTIRFEEEKKNLIDNVVNTLEEYKDVIDSELRTIRNTNHL
                     VMRNNLVLLQS"
BASE COUNT         5669 a         1658 c         2353 g         3956 t
ORIGIN      
        1 tctttactgt ttgtgagtta tttaagtgag gtgttgccta atacacaaat tatttattac
       61 ttttaatttg ttctaatcct ttttttaaga tttctctacc agcttgtgaa acgttatcga
      121 aatctttttc ttgtgcgtat ttgataatct catctttcat atcgggatca actaacaaat
      181 gtagtgatac ttttttcttt ttaccaaaaa agactgaatt ttcttcatta ttattcatag
      241 taatataacc tcctttttgt gctttcgtgt aattctattt aattacacaa taccacattt
      301 acacatgaca tgtcatgaca aaaaagttaa aaaagtttta taaattacat gacatgacat
      361 gtaatactat gttataattg atttatcaaa tagaaaaggt gggttgtaat tggataacat
      421 aacatttcgt aaacgtattg aagaatatcg aaagcgatct attgagcaaa agaaatatga
      481 gagcaaacta aattatcaat caagatttgg tgtgaaagat gatgaagtta gtacttttaa
      541 tatcaaaggc attgctacag ataaaaaaga aaataagtta gtcatggccg aagatagaga
      601 ggataaacaa aaagttcttt tatacgccaa taaagataat gatatgtatt atttgaattc
      661 tggtagcact tataaagagg ctgttcaaaa cacttttggt aatgaaacca attacgaaat
      721 ggtagatgat gaattattta tttataattc aaaaaatcaa gcacttacaa actatgacca
      781 acttgatgag tatcacaatc gacaaaagca attacaatat aacaccaaat tagaacagga
      841 acgaaagcaa agattgactg atacaaataa agttagtgaa aatgctaaag aagatttagc
      901 gttaagttta ggacttacag caacagcatt gaaaggtaac ataaacattt ctaatgttga
      961 aaaagtcact gataatgaaa gtatggttac tatagaaaat gaaattaatg gtaagtcaga
     1021 acatttaatt tcagacttta aagatagttt cattgaaaag gctaaagaaa agctaactgt
     1081 ttccccttca atgaggttag gatttgaaga atttaaaaac gcctcgggac aaatagcaat
     1141 gggatttaca gagttttttg gcgctaggta tgacgaaagc actttagaaa acgaccctaa
     1201 agttgtggca acagaaaaag ggtactctgt ttctgttttt gataaagacg gaaacaaaaa
     1261 agatttaaca tcagctgaca cttttgagga ggcagtaaaa ttatccaaag aaatgaaaca
     1321 tgataactcg ctttctcgta gtgaaataga attaaatcaa gaattagaaa agaaaaaaat
     1381 ataaggaagg ttgattaatt atgattaaaa aattcagttt aacaactgtt tatgtagcat
     1441 ttttaagcat tgttttatca aatataacac ttggcgcaga aaatccaggg ccgaaaattg
     1501 aacaaggttt acaacaagta caaacattct taacaggcct aattgttgct gttggtatct
     1561 gtgctggtgt ttggatagtt cttaaaaaat tacctggaat tgatgatcca atggtaaaaa
     1621 atgaaatgtt tagaggcgtt ggaatggtat tagctggtgt ggctgttggg gcagcacttg
     1681 tttggttggt tccatgggta tacaaccttt tccaataaac acaacaaaag ctacattcta
     1741 gtaatgtagc tttttgtttt aaaaaggagt gaattatatg tctgttgagc aagaagttaa
     1801 agatataatg ctcgatatgt tgggcgaaga aaagaaagtt aaagagaata taatacctcg
     1861 taatgtagac gctagttata aaatattctt aaatttaaac ggtaaagaat tagcaattgt
     1921 atttgttcct tttttactat ttctaacaat agcatttggt gtaacgtttt taatgggctt
     1981 acttaattta ataactggct ttatggtttt tatagtaggt ttgatttttg gtttaacaat
     2041 atatggctta ctaacaatta gaccaattag tacaaaagaa aatataagaa tgattgatac
     2101 cataaaacaa tcacaacgat ttagcagaag gcagaaagtt tacttctata aaagtaaaga
     2161 aggattgggt gatgatgatg tttaaaaaaa agaataagca aaataaaaat aacgaacaac
     2221 aatttgaaat tataaataga tatataaaag gggacggacg taatcaaagt attcaagaag
     2281 attcactaat agctggtttt tataaagatt acgttgttac taaagacggt tatatcagtg
     2341 gaggtttaaa tattactggt gtaaatacag agttattaac gaagtttgag caaaatgatt
     2401 tattagaaga atattcatct tttatttcaa acattcttta cgacgaacca atgataaaaa
     2461 caattacaga accagtaaat atacaaaatt atataaaaca cctttacaga caagtattac
     2521 ttgatatgaa gaataataat aataaaccaa caagacgtgg ccaattaata gctggtcgta
     2581 tattgtttct tcaagaaaag ctagcaaaag gcgaaatgag tacaaagaaa cattatattt
     2641 tcttttcgga aaagataaca gaaaatactg tagcacaatt agatcatgca gtgaatagac
     2701 tacatgaaaa gataaacctt aataaagagg cattacaaca agcattttca cattatggtt
     2761 tagaaactag acctatatat caaacagaat acttaaacca gttttatgct ttatatgatt
     2821 ataaaggtta taacgactat catcttagta attagaacga agggagttta tgattatggc
     2881 atttttaaaa aagaaaaagc aagagcaagt tacaaataaa attcacaatg aatcttttca
     2941 aaaattaaca gaagtattag atacagattc tgttgatagt atttatcctt ttagttggat
     3001 agagaaaaaa tcgcatatag aaacaggcga aaattatatt aaaaatttat tagtagttga
     3061 ctaccctcaa tcagtaaaag gggcgtactt atcaaattta ttaaagaaaa acggtaatat
     3121 tcaaataact aaatttatta gaccagcaaa tatagaaaga atgattgacc atttaaacaa
     3181 ctcaatcaaa aacaaaacag cagaacaaat gcgtactaca gaccctaaac gaaatgcaac
     3241 aattaaaaga gaaattgaaa gtagtaaaaa acaacttgat aaatttttag atgaaaaaac
     3301 aggatttatg tacatgtata tgtatatcac gttaaatggc gattcatacg aaaagataca
     3361 agcgctagaa aaagatgtta agcgtacttt aacgagatta agacttaaaa cacatacacc
     3421 tacaaacgca atgcgagaat catttcatac agttcttcct ttaaatagaa acttcttatc
     3481 agcatttaca caacaaaata tggatacagc aacagcaggg catttcttta tgtttgatga
     3541 ttcagaaatt attgatttaa cacctaatac atcagttttt ggaattaaca aaaatacaga
     3601 ttcattagta gcagttgatt ttaataacaa agaaaaaact ttaaataaaa atatgactat
     3661 tatcggtaca agtggtgtag gtaaatctac cctcaatatg agaatgattt tagacaatat
     3721 caaaaaatct ataagacaat ttatcattga tcccgaagat gaattttcat atataactaa
     3781 atattacggt ggaacagttg taaatatttc aacaagttca aatatcaaaa ttaatccatt
     3841 tgaaattttt tcagaagaag tatttgaaaa agaagatgaa acagacaatg aaacaacatc
     3901 agatgtttta acggaaaaca acgaacacga aagccaaatt gacacattag ttcgttctaa
     3961 aatagggaag ttaaaaacct tctttagagt tcttaaagat gaaatatctc aaacagaaat
     4021 atctgtatta tcttcaactt taagacagtt atatcaagat aaaggattta aaggtaatgc
     4081 gaaattatca gatttcaaaa gcgaagatta tccaacttta acagaattat ataacaaact
     4141 aaaagactta gacccagaaa aatatgaggt attaaaggac ttaacactta tcatagaaga
     4201 ttacacaatg gaacatggta ctactacaat ctttgacggt tatacaaaca tcaaattaga
     4261 taatgaaatt gttactttta accttaaacc attacaaaca gaaaaagatg ttcaaagtgc
     4321 agcgtatttg aatatattca gtttcttatg ggacgaaata acaaaagata gaacaacaga
     4381 aacagtatta atgactgacg aattacattt cttagcaaca aatgaatata gcttagattt
     4441 ttattatcaa gcatacaagc gtattagaaa atatggcggt ggcgctattg cctctactca
     4501 acaaattaaa gatattctta gaacatcaca agaaataggt tcagctatta ttgaaaatag
     4561 tcatactaaa ttcttctttg gaatggataa cgtaggcgtt gatgatgttg ttgataaatt
     4621 aggattgaaa tttagcgatc aagaaatatc tcatttaact aaaaagaaaa aaggcgaggc
     4681 attattacta tacggaacac aaagagcatt tataagaatt gatttagata gagaagaaac
     4741 aagactttgg aataaagaat tatatgaaac aatttatgaa gaaccagcag acgtagagcc
     4801 gaattatgtt gaacaactag gattaacaga tattgattta gcagagttgg aagaagaatt
     4861 aagacaagca gaaatgattt atgaatagga ggtaatctca tgaaaattag aaattttaat
     4921 gaaggaacat tggtaggaaa acataaccac ttagaatata tcatcaatgt tgatttgtgc
     4981 catatatcag atatagacga aattagatat gacttaacag aacctaatga attgtttttt
     5041 gaagttaaag atttacaaaa ggaaggcgat tattttcata tcatttacaa cactgataat
     5101 gaatataatt ccttcctatc agcaaaaaaa gaaagcaaag cactcaaatt atctttaatg
     5161 gattatgtgt taaaagttga tccattaaag gacaatataa ctgttatgca cccagcgaac
     5221 ctatttttta aaaatattga ggatataaaa ataggattca aaggccatga atatttacct
     5281 aaacctaaaa taaataattt agaacagtat aaattgttaa taatgagtac attatcacaa
     5341 tacacgtttg ataagtatta tcgcaataaa tttgaagttt tatcaaaaga aaaagatgat
     5401 tttttccata aagtaaataa tgcagaaact tttgaaaaat tgaaagagat tgtaagacat
     5461 gaattgaatc aagtacagac agattattta ctgaatgaag aaaaacgtaa aagagaaaca
     5521 cgaaaaaagt acattagaaa tttaatactt ggttttgttg gtattgcaat agttataagt
     5581 ttaatttcat ttatgattat aaacggaaag caacaagaat tagatagcaa aatagcacaa
     5641 gcagacaaac aagaacaaag aagtaaagta tatgaaaatc tttacaatgg caatgttgat
     5701 caagcagtta aaggcatgaa acgtgacgat agctttaaca aaaaagatat tgaaaaaaca
     5761 ttaaaaaaag aaaagaaata tgaagaacta attgaattga gtagtaaaaa cacaccttat
     5821 gtaattgaac aattatataa agaaggtaag cagaataaaa taagagagtt agctttcaat
     5881 tttgaaaata acgatacatt gagtttagaa aagaaaatat tagacaaaga cggaacagca
     5941 tttagtgtag gctcaacagg taaatacgaa gaacaaagta aacgtttggc tttagcgtca
     6001 gctaaagaag gttatgttga atcagctaaa gatattaata aaaaacttaa agataatgaa
     6061 gttgaagaag aaataaataa agctgaaatt aaacaactaa aagaagaaaa agacagcacg
     6121 aaggataaag acaagaaaaa agaaatccaa aaagatatag aggaattaga gaagaaatag
     6181 gagggaggta aagtatcatg tggagtgcta ttgctaaagg aataacaatg ctgatgaaga
     6241 aaaaagcatt atccaaaata ttaaaaccag taatagctat agtaataggc tttttccttc
     6301 ttatttttat aatgattgct tatctagtag gatctttcac taaagaaatt aatgagagtg
     6361 gcgaacaaaa aatgaaagag gctgaatcaa cagcaacagg tgcgtgtact ggtggaagtg
     6421 ttgacggttc gggtatctct gcttttgaaa aaaacgccaa aggtggcgca ttagaaggta
     6481 aaggtaagga tatggttaaa attgctaaaa aaaataaaat accaccaaaa ttatttatag
     6541 caattgtagc ttctgagtca gaatggggaa gaggggcaaa tgcaacaaaa caaaaaaacc
     6601 ccctatctat aatgggggct ggccctctac aagtttaccc ttcaattgaa gaaggacttg
     6661 ataaaggtgc aaaaaattta tatgacttat acatttctga aggtttgaca actcccgaga
     6721 aaatcgggcc aaaatacgca ccagtaggcg ctagtaatga cccagatgac ttaaatagta
     6781 actgggtacc tacagttaag aaaatcatga agtcattcgg tggcaaagag gctaaatgtt
     6841 ctactgaaag cagtggtgga ggttcagacg gaaaaggatt tgattttaaa ggagaattcc
     6901 ctaaacctga caaatcgaaa tataacggtc aatcttatcc atggggacag tgtacttggt
     6961 atgtacatca acgtagaaaa gaaattggca aaccagtacc tttaacttgg ggtaatggtg
     7021 gggactgggg cgataatgca aaagcgcaag gctgggaagt aggtagtaag ccgaaagctg
     7081 gcgctggcgc ctctgtaaaa cccggtaact ttggcgcacc accaccgtat ggacacatca
     7141 tgtttgtcga aaaagttaaa aaagacggtg gaattgtagt tagtgaggcc aatgtaaaag
     7201 gcctaggagt tataagttca agagaatttt caaaagcaga aacacaacgt atgcaattta
     7261 tttatgataa gtaggtgtaa aaaatgaaaa aattattaac tatagttatt ttattcactt
     7321 tggttctggc tggttgtggc caaaatcaaa gtgagaaaaa tagtgaaaaa cataatgaag
     7381 aaaaaacagt agatactaca gaagaaagaa acgatatgat aaaaggtcta attgaatatg
     7441 gttatgaaaa tgatgattat aaaggttata agcatatgaa agatatatta tctgaaaagt
     7501 tgcaaaagca agcagaattg caaaatcaat cagcacaaac taaaggtgta aaaaaagaaa
     7561 ctaaagacat taaactttat caagacgtag ataatgatga tgaattgatt tattctatta
     7621 aactttcaat tacaaacaaa aaagagaaaa actctgacta ctcaatgaga tatgggaaaa
     7681 ttgaattaac ggaagaagaa ggtaaacaaa agatagataa tgtcgaagaa ctaacacaaa
     7741 gaaatttagt aagtatgaat caagaataga aagaggtaat acttatgaac actttaattc
     7801 tatgtgaaaa accttcacaa gcaatggact taagcactgt ttttgcaaaa aagaaaaagc
     7861 aaaatggata tatggaaatt tcagatgaac aattaaatgt aagtggcttt ttaacttggg
     7921 cagttggtca tttagtcgaa ttgaaggaac cacaagaata tgacgaaaaa tataaaaatt
     7981 ttagcacata tcctatattg ttagaaaaag acgattttca atttaaagta tctgacaaaa
     8041 cgaaagatca atttaacaac attaaaaaaa tatttaaaga aaacaagatt gatgaagtta
     8101 ttattgctac tgacccagct agagaaggcg aaaatatagc atataaaatt ttaaatcaat
     8161 tgaaagtaac agataaagtt actataaaaa gattgtggct tacttctaaa gtagaaagca
     8221 gtataagaaa ggcttttaaa aatatattac ctaaagaaaa aacttatgga ttttacaaag
     8281 agggcagggc tagagaatta agtgattggt tagtaggaat taacttatca cgtcatttca
     8341 ctaaaatttc acgagaatta ggaaatgacg gtgttattca tatcggtaga gtatctagtc
     8401 ctacattaaa catggtttac aacagagaga ataacattaa aggttttaaa ggtaaaaagt
     8461 tttataaagt ttcagcgact attaacaaag atgaacaaga agtgaaaaca gagttaaaaa
     8521 acaaatttga cagtgaagat gaattgcacg aatttttgtt tgaaaatgat attacagatt
     8581 taacacaaaa agggttagtt actgatattg aaaaagaaat aggctataca atgccaccta
     8641 aattttatga cttatcagct ttacaagaag atatgaatga taaatataaa ataagtgcga
     8701 aacgaacatt agaaatcgca cagacattat atgagaagaa attaatcaca tatccaagaa
     8761 ctgattcaag atatatcaca gaagatgaaa aagaaatgtt attagaaaat atagactatt
     8821 taaaagaaat aacaaagatt aatttgaata atgaattaac taataacagc ttgattaatc
     8881 cttctaaaat agaagatcat tacgcaatat taataactgg aaatgatttt aacaaagtag
     8941 atttaaaaga agaagaaatc aatgtttata aaagtatatt acaaaatgtg gccatgaatt
     9001 ttatggataa agaacaatat gaaacgacta ctatagaaat cgctgttaaa aagcttatgt
     9061 ttgaagtgaa aggtaaaatt attcaagata acggatttaa agctttgtta aataaacaaa
     9121 aaacttccga agaaacaatt cctaattttg aaaaaaatga agaagtagac attgaattag
     9181 atttgttaga aaaagaaacg acacctccaa aaagatatac agaaaaaaca ttgttaaaag
     9241 caatggccaa tcctatagaa acactagaag atgaaggctt aaagtctaca cttaaagaag
     9301 ttaaaggttt aggcacacca gcaactagag ccgatattat agaaaacttg aagaaaaata
     9361 aatatattca agtacaaaaa aacaaaattt atattactaa aaatggtata ttagcttgtt
     9421 tattattgga aggacattta ctttcaaaac cagacttaac aggacaatgg gaaaaatatt
     9481 tgaatggtat tagtaaaggc gaaaaagatg atgattcatt tataaatacc attaatgaaa
     9541 tgattaaaaa aacaattaat gaagaagtta aaaataaaga aagtatacaa aaagtggcca
     9601 aagagaaagt atcgactaac aatatagcaa aatgccctgc ttgcgataat ggctatttaa
     9661 tagatagaaa aggtttttat ggttgtacag agtacaataa cggttgtgaa tttactattc
     9721 ctaaaaaatt gttagaaaaa agtatacctc caactgtagt aaaagcatta tgtgaaagta
     9781 agacaacaaa aaaattgaaa ggctttaaat caaaaaaaag tggaaagtca tttgattgta
     9841 agttgatatt aacaaaagaa aacaaacttc aattttcatt cgattaaaag gaggataaga
     9901 aagtgaaaaa aggaatattc attttaactg gtattatttg gctagtatta atatatttag
     9961 cgcaaatatt agcatatgct ttaaatacac taagtaatac tagcaataag caaaagatat
    10021 ttagtgatat atcacagttg tttatacctc cagctaaaca cccaataagc actattatcg
    10081 gttttgtatc tgataaaaat acagtgtttt ttgtaatagg tgtaatagca acaattattt
    10141 gtatatatat tcttataaaa caattatttg agagcaaaga cggaaacgaa aaaaatcaag
    10201 actataaaat tgctaaacat ggttctcatg gatcagctaa atttgccgaa gatagcgaat
    10261 tgttttcaga aggacattat aaaaaaatga aagaaaacga cattacgaac tatgtttata
    10321 acagtttaga taaatcatta atagaagaaa gaagtgataa ggaatgacta cacttttagg
    10381 cgaattagaa agtaaagcaa cttctataaa taaaaagaat ctagtaatac aagattttga
    10441 tactaaatgc cctaatagaa atatatttgt ggtaggaggg ccaggttcct ataagtcagc
    10501 tgggtatgtt attcctaatg ttattgttaa aaatcaacaa tcaatagttg taactgaccc
    10561 tagtggcgaa gtttatgaaa aaacatctaa tattaaacgc atgcaaggtt ttgatgttag
    10621 agtagtaaat ttcaaaaact ttttagcctc tgatagacaa aacttttttg attatattga
    10681 taaagattct gattgttcaa tagttgctga attaattatt aaatcagctg gggattctaa
    10741 aataaataaa gacgtatggt ataaagcctc tgtaggatta ttaaattcat tgcttttata
    10801 cgctaaatat gaatttgaac cagaaaaacg aactatagga aacattatta aatttattca
    10861 aaataacaaa cctaatcaag atgaagaagg atcagttgaa ttagataaac gctttaatga
    10921 attatctaaa gaccacccag ctagagaaag ttatgaattt ggttttgcag tatcagaagg
    10981 aagaacaaga gcaagtatta tactaacctt tgtagccgat ttacgaaatt acattgataa
    11041 cgaaattaga agttatacaa gcgattctga ttttgattta cgagatgtag gattaagaaa
    11101 aactattata tatgtaatgt tgccagtttt ggggaacact gttcaatcac tttctagttt
    11161 attcttctct caaatgttcc aacagctata taggttaggg gacgaaaacg gtgctagact
    11221 tccagtccca gtagattttt tactagacga atggccaaat atcggcgcaa taccagacta
    11281 tgaagaaact ttagcaactt gtcgtaagta tggtattagt atcacaacaa ttgttcaatc
    11341 tatatcacaa gcagtagata aatataataa agataaagct aacgctatta taggtaacca
    11401 cgcagttatt ttatgtttag gtaatgtgaa taatgataca gcaaaatata taaaagaaga
    11461 attaggtaat gctactgtag agtttgaaac aagtagtgaa ggttcttcta gtggtaaaga
    11521 tagcagaagt aaatcatcaa ataaaaatgt aagctacact ggaagggcat tactaaatga
    11581 agatgaaata agtaatatgc aacaggataa agctattctt ataactaaaa atagaaatcc
    11641 taaaattatt aaaaagttag catattttaa aatgttccct aatttaacag aaacgtatat
    11701 agcacctcaa aacgaataca aacgtaaaaa aaaccagtct atgattgata agcataatag
    11761 attaagagaa gaatgggata aaaaacaaga gaaaaataaa cagcaaaaac tagaagaata
    11821 taaagaagaa caaagacaga aagagttaga gaaagaacaa caaccacaag aagaacaaca
    11881 aaatgagaaa gtaaaagaaa gtaaagacaa aaacgatcaa gaagataaga aagacgagca
    11941 acaaaaaatt aatgattcta agaaagcatt ctacgaaaaa ttaaaacaaa aacaagctaa
    12001 ataaaagtaa gattttgcat tacatgacat gacatgtaat gcaaaatctg ttatacttgt
    12061 attgaaagta gaggtggttg aatggattgg attaaagacg ctattaaaga actgaccgaa
    12121 ggtattgcag acggtatgat gaattttata tttcagtttc tttatacgtt attttacgaa
    12181 cctttaaaat tctttatatg gttgttagat aaaatactta ttgatcctaa tagtatttca
    12241 agtgattaca tttcaaacgc ctctgtatta gttaatagct taatcacttc aatagcaata
    12301 tgtgtgtttg cttttaaaat ggttcaagtt atgaaacaaa atgcagaagg taatgctgaa
    12361 agtccaggct attatgtagc acaattatta ccaagttctt tattggtagc agttttacca
    12421 tggctagttg atataatgac taaattatct tattcagcga ctagggcatt tttagatgta
    12481 ggtcaaaaaa gttatttaga aactattaaa gaatggagtt ctttaaaaaa aggtcaagac
    12541 ggatttaaag aaatgctagg ttctatgggg actatggcag gtagtcaaat tatgtttatt
    12601 ctatttattc tattgatttt agtatttact gtagtattta ttttccaatt tgttagtagg
    12661 atagctgatt tagtaataat gaaagttgtt gccccgttag tagcagtttc tttattagca
    12721 gatgaaaata attatatagg tgtatggtgg cgtgaattat tagcaattag tattcaatta
    12781 ccattacaag tgtttacttt ctttggagga ataaatttat tctttggtgc aaagctagac
    12841 gtaggcacat tattattagg tataggattc tttattatta cagttaaatc gccttccttt
    12901 attagatcaa tggtttactc tactggaagt gggcgcatgg caactggaat ggctggaagt
    12961 ggtgcaaaac ttctagttag aaaaatgtta tctaaataaa gaaaggaaga tatttatgat
    13021 aaacaaagta atatatagtg gacgtatcgc aactgattta aaattaagaa aaagtaaaaa
    13081 tgaaaagtct tatgttttct ttaacatagc tgtacctagg cagtttgata atgatagaac
    13141 agattttatt aattgtgttg tatggggaaa acaagcagaa agtttagttg attatcaaag
    13201 taaaggtaat ctaattatta ttgaaggttc aactgaaaca tataaaaaag acaatattaa
    13261 tatgtctaga tgtttggttg aaaagactat ctttttagaa agcaaaaaag aaaacaaaaa
    13321 acatgacgtg tcagaagatg acgctataag cccagcagaa gatagtggca acgataaatc
    13381 tatctctgat gatgacttac cattttaaaa ctataaaagg acgtgtgaaa acacgttctt
    13441 ttttaaaaaa taggagtgat ttagatgaaa gtaacaaata caattagatt tgaagaagaa
    13501 aagaaaaact taattgataa tgtagtaaat acactagaag aatataaaga tgttatcgac
    13561 agtgaattgc gtactattag aaacactaat catttagtta tgagaaataa tttggttctg
    13621 ttgcaaagtt gaattt
//
LOCUS       DAKTIO010000062        13542 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.062, whole genome shotgun sequence.
ACCESSION   DAKTIO010000062 DAKTIO010000000
VERSION     DAKTIO010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13542
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.062"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..285
                     /locus_tag="O0293_001361"
     CDS             <1..285
                     /locus_tag="O0293_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HCY0280250.1"
                     /translation="KDLSKYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYDIPT
                     NKAPKLLLKGTGNLKGSSVGYKDIEFTFVEKKEENIYFSDGLIFKPSEDK"
     gene            352..1119
                     /locus_tag="O0293_001362"
     CDS             352..1119
                     /locus_tag="O0293_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0280251.1"
                     /translation="MKRLNKLVLYISFLILVISFTAGCGIGKEAEVKKSFEKTLSMYP
                     IKNLEDLYDKEGYRDDEFDKNDKGTWIIGSEMVVQPKGERMKSKGMVLYMNRNTKTTT
                     GKYIVSETLHDEDGRPKSKDKEYPVKMVDNKIIPTKGIKDENIKKEIENFKFFAQYGS
                     FKDLSKYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYKIPTNKAPKLLLKGSGD
                     LKGSSVGYKDIEFTFVEKKGENTFFTDSLHLEPSEDK"
     gene            1213..3450
                     /locus_tag="O0293_001363"
     CDS             1213..3450
                     /locus_tag="O0293_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCY0280252.1"
                     /translation="MQRDYLIRVETESMSDFKRLNSLMIGFVIKGEAHIYDENNMTQC
                     NSGDIFIINHRDLYRFQLQQDGIICYIQFQMKYLADKFDDVHCLYFHLTDATTTKNIH
                     QLRNIMARLVSTHIRHNELSKLTEQQLVIQLLMHMIHYVPRTYHSNQSILNDDKVNQV
                     CDYIELHFHEDLSLSELSEYVGWSESHLSKKFTESLGVGFHHFLNTTRIEHAKLDLTY
                     TDETITDIALQNGFSSAASFARTFKHFTHQTPKQYRGDRPAITENQQSAQHDYHDREL
                     ILLLNDYIEEMNHFIEDIEKMNYKEIAFQPTNHQLNQFNHIIQVGYLRNLLNTQYQSQ
                     LLTCHHDFQVNEVLAYDVIPYIMKKLNAPFTYDAEISNIFYDIDLCLDFLLDHNFSLT
                     MHLDQYDSRDYIDAFKIFIHHVALHVSHRKDLKFNLYVTTLHTSLIEMIDYFKALFPN
                     GGLYIHLDQATERHLPLLKRLEPHIDHFVFDANSNDAVDFNKMNDDEFKTASQMIINK
                     TNYLIDLMHRHRLKRPLILLNWNTLTGDTFITNGEYFRGGIIIEQLLKLSSKVEGIGY
                     WLNYDLHVSHCKNERDYMNSIELFHQYNGKRPVYFTALLFNKLTSNILYSDDTCIVTG
                     TDSNFQILLYDAKHFNPYLALDNQMNMRATEMIHLNINALEEGMYKIKHFTLDKENGA
                     LFNLWRKHHTIHGMDKDSIDYVNRMSFPKLEVYDIDITDTLALNIKMITNGIHLIEVK
                     RYPSS"
     gene            3601..4779
                     /locus_tag="O0293_001364"
     CDS             3601..4779
                     /locus_tag="O0293_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCY0280253.1"
                     /translation="MNQQLIETLKSKEGKMIEIRRYLHQHPELSFHEDETAKYIAEFY
                     KGKDVEVETNVGPRGIKVTIDSGKPGKTLAIRADFDALPITEDTGLSFASQNKGVMHA
                     CGHDAHTAYMLVLAETLAEMKDSFTGKVVVIHQPAEEVPPGGAKTMIENGVLDGVDHV
                     LGVHVMSTMKTGKVYYRPGYVQTGRAFFKLKVQGKGGHGSSPHMANDAIVAGSYFVTA
                     LQTVVSRRLSPFETGVVTIGSFDGKGQFNVIKDVVEIEGDVRGLTDATKATIEKEIKR
                     LSKGLEDMYGVTCTLEYNDDYPALYNDPEFTEYVAKTLKEANLDFGVEMCEPQPPSED
                     FAYYAKERPSAFIYTGAAVENGEIYPHHHPKFNISEKSLLISAEAVGTVVLDYLKGDN
                     "
     gene            4781..6169
                     /locus_tag="O0293_001365"
     CDS             4781..6169
                     /locus_tag="O0293_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0280254.1"
                     /translation="MNETYRGGNKLILGIVLGVITFWLFAQSLVNVVPNLQQSFGTDM
                     GTISIAVSLTALFSGMFVVGAGGLADKIGRVKMTNIGLLLSIIGSALIIITNLPALLI
                     LGRVIQGVSAACIMPSTLAIMKTYYQGAERQRALSYWSIGSWGGSGICSLFGGAVATT
                     MGWRWIFIFSIIVAVLSMLLIKGTPETKSEITNTHKFDVAGLIVLVVMLLSLNVVITK
                     GAALGYTSLWFFGLIAIVIVAFFIFLNVEKKVDNPLIDFKLFENKPYTGATISNFLLN
                     GVAGTLIVANTFVQQGLGYTALQAGYLSITYLIMVLLMIRVGEKLLQKMGSKRPMLLG
                     TFIVVIGIALISLVFLPGIFYVISCVVGYLCFGLGLGIYATPSTDTAISNAPLDKVGV
                     ASGIYKMASSLGGAFGVAISGAVYAGAVAATSIHTGAMIALWVNVLMGIMAFIAILFA
                     IPNDDKRVKDAK"
     gene            complement(6662..8323)
                     /locus_tag="O0293_001366"
     CDS             complement(6662..8323)
                     /locus_tag="O0293_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCY0280255.1"
                     /translation="MSVTEVIFSFLGGLGIFLYGLKIMGDGLQASAGDRLRDILNKFT
                     SNPVLGVIAGIVVTILIQSSSGTTVITIGLVTAGFMTLKQAIGVIMGANIGTTVTAFI
                     IGIDLGEYAMPILALGAFLIFFFKRSKINNIGRILFGFGSLFFGLEFMGDAVKPLASL
                     DGFKQLMLDMSTNPILAVIVGAGLTALVQSSSATIGILQEFYQQDLISLNAAIPVLLG
                     DNIGTTITAILASLAGSIAAKRAALVHVIFNLIGVIIFTIFLPVVIHLISLLQDLWHL
                     KPAMTIAVSHGIFNITNTLIQLPFVAGLAWIVTKLVPGKDIADDYKPQHLNKDLVYHA
                     PGVALQETQKELQNVGQIVLSMFEDIREITKDDKKLIKKLEQKHQAVETINDSIRNYL
                     VRISTKAITKADVERLAVMFDVNRSILKVAELTEEYVAQLKRQHDEDIRITEDAQRGM
                     DKLFNHVAESFDKAIDMLDVYDKTKKDEIVERSRESFNIEHKLRKGHIKRLNRGECTT
                     KGGLLYIDMIGVLERIGYHSRNVSEALVGLNDDVPTDEEIATTEI"
     gene            complement(8649..10424)
                     /locus_tag="O0293_001367"
     CDS             complement(8649..10424)
                     /locus_tag="O0293_001367"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HCY0280256.1"
                     /translation="MYYSYGNYEAFARPKKPENVENKSAYLIGSGLASLAAACFLIRD
                     GQMEGSKIHILEELPKAGGSLDGENMPLKGYVVRGGREMENHFECLWDLFRSIPSLEI
                     DNASVLDEFYWLNKEDPNYSRCRVIEKQGQRLVTDGDFTLTKTAIKEILDLCLTNEED
                     LDDVKITDVFSDDFFNSNFWIYWKTMFAFEPWHSAMEMRRYLMRFVHHISGLADFSAL
                     KFTKYNQYESLVLPMVEYLKSHGVQFEYDVKVEDIKIDVTTSQKIAREILIDRNGNAE
                     SIKLTINDLVFVTNGSITESSTYGDNDTPAPPTDELGGSWTLWKNLARQSPEFGNPDK
                     FCQNIPKKSWFVSATSTTNNKEIIDTIESICKRDPLAGKTVTGGIITINDSAWQMSFT
                     INRQQQFKDQPENEISTWIYALYSDVNGDYIKKPITECSGNEICQEWLYHLGVSTDKI
                     EDLAKHASNTIPVYMPYITSYFMTRAIGDRPLVVPHQSQNLAFIGNFAETERDTVFTT
                     EYSVRTAMEAVYQLLNIDRGIPEVINSPFDLRVLMDAIYELNDHQDLREITKDSKMQK
                     LALAGFLKKIKGTYIESLLKEHKLL"
     gene            complement(10598..11470)
                     /locus_tag="O0293_001368"
     CDS             complement(10598..11470)
                     /locus_tag="O0293_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCY0280257.1"
                     /translation="MNKLQDTTFLSYLFTIILWGSAFPMIKIALNDFSAESLSAFRLI
                     LATIILLPFVIIKKLPTPELRDIPVIFILGFCGFVIYHTALNFGEALISAGISGILVS
                     TTPIFSSALAYIFLKEHFSKWNWLSSLVAFIGISIISISKDDYTTINVLGVFIILLAS
                     FSESLYFTFQKKYIEKYGFIAFTLYTIMASSPFMLIFIPEIINDIHGATFTSIVSVLY
                     LAIFPTIIPYVLLAYIVKSVGVSDATMSLYLTPIVSLLLSYLLLDELPTTLAIIGGII
                     TLLGVSLSNFFQNT"
     gene            11583..12980
                     /locus_tag="O0293_001369"
     CDS             11583..12980
                     /locus_tag="O0293_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCY0280258.1"
                     /translation="MAKYKDIASDIRDKIITGDWFYGMKIPSHRQLAIQYNVNRVTII
                     KSIELLEAEGFIYTKVGSGTYVNDYLNEAHITNKWSEMMLWSSQQRSQYTVQLINKIE
                     TDDSYIHISKGELGISLMPHIQLKKAMSNTASHIEDLSFGYNNGYGYIKLRDIIVERM
                     SKQGINVGRENVMITSGALHAIQLLSIGFLGQDAIIISNTPSYIHSTNVFEQLNFRHI
                     DVPYNQINEIDTIIDRFINFKNKAIYIEPRFNNPTGRSLTNEQKKNIITYSERHNIPI
                     IEDDIFRDIFFSDPTPSIKTYDKLGKVIHISSFSKTIAPAIRIGWIVASEKIIEQLAD
                     VRMQIDYGSSILSQMVVYEMLKNKSYDKHLVKLRYVLKDKRDFMLNILNNLFKDIAHW
                     EVPSGGYFVWLVFKIDIDIKYLFYELLSKEKILINPGYIYGSKEKSIRLSFAFESNEN
                     IKHALYKIYTYVKKV"
     gene            13170..>13542
                     /locus_tag="O0293_001370"
     CDS             13170..>13542
                     /locus_tag="O0293_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HCY0280259.1"
                     /translation="MSKIRSFTILSLLIYLAMMCYTVVTYPKLPTKVPIHYNLAGDAD
                     NFADKWVLLLINSAFIVIWLIFFIAGRYYERFAKWSHYNHTPREIRAIKLFLSTLNLE
                     IMSYMSIFTVLEIWQIQHHHQF"
BASE COUNT         4665 a         2072 c         2427 g         4378 t
ORIGIN      
        1 aaagatttgt cgaagtataa agacggagat atttcatata atccagaggt gccgagttat
       61 tcggctaaat atcaattaac taatgatgat tacaatgtaa agcaattacg taaaagatat
      121 gacataccaa caaataaagc accgaagttg ttgttgaagg gtacagggaa tttaaaaggt
      181 tcatcagttg gatataaaga cattgaattt acgtttgtag agaaaaaaga agagaacatt
      241 tactttagtg atgggttaat ttttaaacca agtgaggata aataatcatg actcgatcaa
      301 aatatgaaat caatccagga aagataataa gaagaatagg attgtgaaat tatgaaacga
      361 ttaaacaaat tggtattgta tattagtttt ttgatattag tcattagttt cactgctggt
      421 tgtggcatag gtaaagaagc ggaagttaag aaaagctttg aaaaaacatt gagtatgtat
      481 ccgattaaaa atctagagga tttatacgat aaagaaggtt atcgtgatga cgaatttgat
      541 aaaaatgata aaggtacatg gattattggt tctgaaatgg ttgttcaacc taaaggggaa
      601 cgtatgaaat caaaaggtat ggttctatat atgaatagaa acactaagac tacaaccggg
      661 aagtatatag tgagtgaaac acttcatgat gaagatggta gacctaagag taaagataaa
      721 gaatatcccg ttaaaatggt agacaataaa atcattccaa ctaaaggtat caaagacgaa
      781 aatataaaaa aagaaatcga gaattttaag ttcttcgcgc aatatggcag ctttaaagat
      841 ttgtcgaagt acaaagacgg cgatatatca tataatccag aggtaccgag ttattcagca
      901 aaatatcaat taactaatga tgactataat gtaaaacaat tacgaaaaag atataaaata
      961 ccaacgaata aagcaccaaa gttattgttg aaaggttcgg gagacttaaa aggttcatca
     1021 gttggatata aagacattga atttactttt gtggagaaaa aaggtgaaaa tacttttttt
     1081 actgatagtc tacatcttga accgagtgag gataaataat catccacaca cacgattcaa
     1141 tatgaaattc aaatatgttg ctgtaaaata atgtaaaata aacgtattca tattactgaa
     1201 ttgagggata gtatgcaacg tgattattta attcgagtag aaactgagag tatgtcagat
     1261 ttcaaaaggc tcaatagttt aatgattggt tttgttatta aaggtgaggc acacatttat
     1321 gatgaaaata atatgacgca atgcaacagt ggcgacattt tcatcattaa ccaccgcgac
     1381 ttgtatcgat ttcaacttca acaagatggc atcatatgtt atatccaatt ccaaatgaaa
     1441 tatttagcag acaagtttga tgatgtgcat tgtctatatt ttcacttaac agatgcgacc
     1501 acaacaaaga atatacatca actgagaaat ataatggcaa gactggtttc aacacatatc
     1561 cgacataatg agttgtctaa attgactgag caacaacttg tgattcagtt gcttatgcat
     1621 atgattcatt atgtcccgcg tacatatcat tcgaaccaaa gtatcttaaa tgatgataaa
     1681 gtgaatcaag tatgcgacta tatcgagtta cattttcatg aagatttaag cctttcagaa
     1741 ttaagcgaat acgttgggtg gtcagagagc catctgtcta aaaagtttac agaatcgcta
     1801 ggtgtaggat tccatcattt cttaaatacg acgcgaattg agcatgcgaa actcgatttg
     1861 acatacacag atgaaaccat tactgatatt gcattgcaaa atggcttttc aagtgcagcg
     1921 agctttgcga gaacatttaa acactttacg catcaaacac ctaaacaata tcgaggtgat
     1981 cgtccagcaa tcactgaaaa ccagcaatcg gcacaacatg attatcacga ccgtgaattg
     2041 atattacttt taaatgacta cattgaagaa atgaatcatt ttattgaaga tattgaaaag
     2101 atgaactata aagagattgc ctttcaacca accaatcacc aactaaatca atttaatcat
     2161 attattcaag tgggctattt gaggaatttg ctcaatacac agtatcaatc acagttgctt
     2221 acatgtcatc atgattttca agtcaatgaa gtattagcat atgatgtgat tccatatatc
     2281 atgaaaaagc tcaatgcgcc attcacgtat gatgcagaaa tttcgaatat attttatgat
     2341 attgatttgt gtttagactt tttattagat cataacttta gtttgaccat gcatttggat
     2401 cagtatgact cacgtgatta tatcgatgca tttaaaattt ttatccatca cgttgccctg
     2461 catgtcagtc atagaaaaga tttgaagttc aacttgtatg tgacgacatt gcacacgtct
     2521 ttgattgaaa tgattgatta ttttaaagca ttattcccta atggtggctt gtacattcac
     2581 ttagatcaag ctacggaaag acatctacca ttattgaaac gacttgagcc acacatcgac
     2641 cattttgtat ttgatgccaa ttcaaatgac gctgttgatt ttaataaaat gaatgatgat
     2701 gaatttaaaa ccgcgagtca aatgattatt aataaaacga attaccttat cgacttaatg
     2761 catcgtcatc gcctaaagcg tccactcatt ttactcaatt ggaatacatt gacgggtgat
     2821 acatttatta caaacggcga atattttaga ggtggtatca tcattgagca attattaaaa
     2881 ttaagttcta aagtagaggg tatcggttat tggttgaatt atgatttgca cgtcagtcat
     2941 tgtaaaaatg aacgggatta tatgaattct attgaactat ttcatcaata taatggaaaa
     3001 cgtccggtct atttcacggc attgctattt aataaattaa caagcaatat tttgtattct
     3061 gatgatacat gtattgtcac ggggactgat tcaaattttc aaatattgtt atatgatgca
     3121 aagcatttta atccgtactt agcgttggac aatcaaatga atatgcgtgc gacggaaatg
     3181 attcatttga acattaatgc cctggaagaa ggtatgtata agattaaaca ttttacctta
     3241 gataaagaaa atggtgcgtt atttaatctt tggcgcaaac atcatacgat acatggcatg
     3301 gacaaggact ctatagatta cgttaatcga atgagttttc cgaaattaga agtatatgat
     3361 atagatatca cggacacact ggcattaaac attaaaatga ttacgaatgg gattcactta
     3421 attgaagtaa aacgttaccc aagttcataa aatgatcaca aatcacaaat tttgatatac
     3481 ataatttgtg attttttata ttcaaagcta aaattgcaaa aaattaatgg ttaacatctc
     3541 tgttgttggc aatataaata atgattaatc atttatgatg taactaagga gatgaaggat
     3601 atgaatcaac aattaattga aactttaaaa tctaaagaag gcaaaatgat tgagatcaga
     3661 cgttatttac atcagcatcc agaattatct tttcatgaag atgaaacggc gaaatacatc
     3721 gctgaatttt acaaaggtaa agatgtggaa gtagaaacga atgtcggacc acgtggaatt
     3781 aaagtaacga ttgattcagg gaaacctggt aaaacattag caatccgtgc agactttgac
     3841 gcattaccca ttactgaaga tacaggatta tcttttgcat cacaaaataa aggtgttatg
     3901 cacgcatgtg gtcacgatgc acatacagca tacatgcttg tattagcaga gacgcttgct
     3961 gaaatgaaag atagttttac aggaaaagtc gttgtgatac atcaaccagc tgaagaagta
     4021 ccaccaggtg gtgctaaaac aatgattgaa aatggtgtat tagacggtgt tgatcatgta
     4081 ttaggtgtac acgtcatgag cacaatgaaa acaggtaaag tgtattacag acctggttat
     4141 gttcaaacag gacgcgcatt cttcaaattg aaagttcaag gtaaaggtgg tcatggttca
     4201 tcaccacata tggccaatga tgccattgtt gcaggtagct acttcgtcac agcgttacaa
     4261 acagttgtat ctagacgact aagtccattt gaaaccggtg ttgtcacaat cggttcattt
     4321 gacggtaaag gtcaattcaa tgtcattaaa gatgttgttg aaattgaagg tgatgtacgt
     4381 ggattaacag atgctacaaa agcaacaatt gaaaaagaaa ttaaacgttt atcaaaagga
     4441 ttagaggata tgtatggtgt aacttgcacc ttagaatata acgatgatta tccagcatta
     4501 tataatgatc cagagtttac tgagtacgtg gctaagacgt tgaaagaagc aaaccttgat
     4561 tttggtgttg aaatgtgtga accacaacca ccttcagaag actttgcata ttatgctaaa
     4621 gaacgcccaa gtgcctttat ttatacaggt gcagcggtgg aaaatggtga aatttaccca
     4681 catcatcatc ctaaatttaa catttcagaa aaatcattac ttatttctgc agaagcagta
     4741 gggacagttg ttttagatta ccttaaagga gataactaac atgaatgaaa cgtatcgcgg
     4801 gggcaacaag ttaatcttag gtattgtatt aggtgttatt acattttggt tgtttgcaca
     4861 atcacttgta aatgttgtac cgaatttaca acaaagtttt ggtacagaca tggggacaat
     4921 tagtattgcg gtcagtctaa ctgcactatt ttcaggcatg tttgttgttg gagcaggtgg
     4981 tctggcagat aaaattgggc gcgtgaaaat gacgaatatc ggtttattat taagtattat
     5041 tggttcagca ttaattatta ttacgaattt accggcatta ttaattttag gtcgtgttat
     5101 acaaggtgta tcagcagcgt gtattatgcc ttctacattg gccattatga aaacttatta
     5161 tcagggtgct gaacgtcagc gtgccttaag ttattggtct atcggttctt ggggtggcag
     5221 tggtatctgt tcactcttcg gtggtgcagt tgcgacaact atgggttgga gatggatttt
     5281 catcttctca attatcgttg ccgtactttc aatgttactc atcaaaggga cgcctgaaac
     5341 gaaatcagaa attaccaata cacataaatt tgacgttgca gggctaattg ttctagtagt
     5401 tatgttgcta agtttaaacg ttgtcattac taaaggtgca gcacttggtt acacatcatt
     5461 atggttcttt ggtttgattg caatcgtaat tgtagcattc tttattttct taaatgttga
     5521 gaaaaaagta gataatccac ttattgattt taaattattt gaaaataaac catatacagg
     5581 tgcaacgatt tcgaacttct tattaaacgg tgttgcaggt acattaattg tagcgaatac
     5641 attcgtgcaa caaggtttag gttatacagc attgcaggca ggatacttat caattactta
     5701 tttaatcatg gtgttattga tgattcgagt tggtgaaaaa ttattacaaa aaatgggttc
     5761 taagcgacca atgttattag gtacattcat tgtggtcatt ggtattgcac ttatttcatt
     5821 agtattctta ccaggcatat tttatgttat cagttgtgtc gtaggatatt tatgtttcgg
     5881 actaggctta ggtatttatg caacaccttc tacagataca gctatttcga atgcaccgtt
     5941 agataaagtt ggcgttgctt caggtattta taaaatggct tcatcacttg gtggcgcatt
     6001 cggtgtcgca attagtggtg ctgtatatgc tggtgcagtt gctgcaacga gcattcatac
     6061 aggtgcgatg attgcacttt gggttaacgt attaatggga atcatggcat ttatcgcaat
     6121 tttattcgcg attcctaatg atgataaacg tgtcaaagat gcgaaataat aacgatgaca
     6181 cgccaagtgt tttgataatg atataaatga aagtaaagca aattaaatga ggaaatgtat
     6241 agagcagtgc atatagataa atctatgatt ggattaacac taatataaat ataaatgaaa
     6301 atgaaaaacc atccacttaa atttttgata agttaggtgg atggttattt attgcgttgt
     6361 tctaaaaatt tggacattta tatggaaaga actatatata atgtgaaggt tatggcaatt
     6421 cgcttcaatg aaggcgacgt gtaaaagtgt atattaaacg tttcggggtc agactccgag
     6481 aacagtgtcc gtcatgctta ccgcatacta atgaagcatt attatatcag tgtttatagc
     6541 cgatttaaga taataaattt tgctaaacaa aaaagccaac ccatgaatgt tggattggct
     6601 ttttacatgc atctgaatct ctaattttaa aaaaatatga atataaataa gacagtaaaa
     6661 attaaatttc agttgttgca atttcttcat ctgtaggtac atcatcgtta aggccaacaa
     6721 gtgcttcaga aacatttcgt gaatgataac cgatacgttc aagaacacca atcatatcga
     6781 tatatagtaa tccgcctttt gttgtacatt caccacgatt aaggcgttta atatgacctt
     6841 tgcgtagttt atgttcaata ttaaatgatt ctctactacg ttctacaatt tcatcttttt
     6901 tcgttttgtc ataaacatct aacatgtcga tggctttatc aaatgactca gcaacatggt
     6961 tgaataattt atccataccg cgttgtgcat cttctgtaat gcgaatatct tcatcatgtt
     7021 ggcgttttaa ttgagcgaca tactcttctg ttagctctgc tacttttaaa atagagcgat
     7081 tgacatcaaa cataactgct aaacgctcaa cgtctgcctt cgtaatggct tttgtagaaa
     7141 ttctaactaa ataatttcga atgctatcat tgattgtttc aacagcttga tgcttttgtt
     7201 caagcttttt gatcaatttt ttatcgtctt ttgtaatttc gcgaatgtct tcaaacattg
     7261 ataagacaat ctgacccaca ttttgtaatt ctttttgagt ttcttgtaat gcaacaccag
     7321 gtgcgtgata aacaagatct ttgtttaagt gctgaggttt atagtcatca gcaatatctt
     7381 tacctgggac aagctttgta acaatccatg ctaaacctgc tacaaatggt aattgaatca
     7441 aagtatttgt tatgttgaag ataccatgtg atactgcaat cgtcatcgct ggttttaagt
     7501 gccataaatc ttgtaacaaa ctaatcaaat gaatcacaac tggcaagaaa attgtgaaga
     7561 taattacccc gattaagtta aagatgacgt gtacaagcgc cgcacgtttt gcagcgattg
     7621 agccggctaa actagctaag atagctgtaa tcgtggtacc aatgttatcg cctagtaaca
     7681 cagggattgc tgcgtttaag ctaattaaat cttgttgata aaattcttgt aaaataccaa
     7741 tcgtcgcact tgaactttga actagtgctg ttaaccctgc gccgacaatg acagcgagta
     7801 ttggatttgt agacatatca agcattaatt gcttaaatcc atctaatgat gctaaaggtt
     7861 taacggcatc acccataaat tctagaccga agaatagtga accgaaaccg aatagtatgc
     7921 ggccaatgtt attgatttta gagcgtttaa agaaaaagat taagaatgca cctaatgcta
     7981 aaattggcat tgcatattcg cctaaatcta taccgataat aaatgcagtt accgttgttc
     8041 cgatattagc acccattatc actccaatgg cttgtttcaa tgtcataaat ccagctgtta
     8101 ccagtccgat tgtgataact gtcgtacctg aactactttg tattaaaata gttacaacga
     8161 tacctgcaat aacacctaat actggatttg atgtaaattt gtttaaaata tctcgtagcc
     8221 tgtctcctgc tgatgcttga agcccgtctc ccatgatttt taagccgtaa aggaaaatac
     8281 ctaaaccacc taaaaaggag aaaatgactt ctgtaaccga catttccatt atttcacctc
     8341 aaataagctt tatatttaga ttatcgctta taattgtaaa tttaatgtta agattaggta
     8401 aaattattta acaatatatg ttatttgtat atgacttgta aaatatcgtc acttattatg
     8461 taaattttca gtgtgaaatg gcaggtttgc aatcacttgt ttaacaaaat gatgcaatca
     8521 atcatgtaat tatgtttcat caaaaaaatc atgagagtgg aacaacgaaa taatttttat
     8581 gaaaacatca tttctgtccc agtctcatga tttgaaatca ccaaataaaa atctattaat
     8641 ggttttcgtt ataacaattt gtgttctttt aataatgact caatgtacgt accttttatc
     8701 tttttaagga atcctgctaa tgcgagtttt tgcattttcg aatctttagt aatctcacgc
     8761 aaatcttggt ggtcattcag ttcgtatatg gcatccatta agacgcgaag atcaaatgga
     8821 ctattgatga cttctggaat accacgatct atatttagta attgataaac agcttccatg
     8881 gcagtacgaa ccgaatattc tgttgtaaat acagtgtctc gctctgtttc tgcaaagtta
     8941 ccaataaatg ctaagttctg agattgatgc gggacgacta aaggtctgtc gccgatagca
     9001 cgcgtcatga aataagatgt gatatatggc atataaacag gaatcgtatt agatgcatgt
     9061 tttgctaagt cttcaatttt gtcagttgat acacctaagt gatacagcca ttcttggcat
     9121 atttcattac cactacattc tgtaattggc tttttaatat aatcgccgtt tacatctgaa
     9181 tataaggcat aaatccatgt agatatttca ttttcaggtt ggtctttaaa ctgttgctga
     9241 cgattgattg taaaactcat ttgccatgca gaatcattga ttgtaataat accgcctgta
     9301 actgttttgc ctgcaagtgg gtcacgttta caaatacttt ctattgtatc gataatctct
     9361 ttattgtttg ttgtagaagt tgctgaaaca aaccaacttt ttttaggaat attttggcaa
     9421 aacttatcag gattaccaaa ttcaggactt tgtcgcgcta aatttttcca tagtgtccaa
     9481 ctaccaccta attcgtcagt tggtggcgct ggtgtatcat tatcaccata agtagagctt
     9541 tctgtaatac taccgtttgt cacaaagaca agatcgttta tagtcagttt aatagattct
     9601 gcattaccat tacggtcaat taatatttct cgggcaattt tttgacttgt cgtaacatct
     9661 attttaatat cttcgacttt tacatcgtat tcaaattgaa ccccatgcga ttttaaatat
     9721 tcaaccatag gtaatactaa agattcatat tgattatatt tagtgaattt taaagctgaa
     9781 aagtctgcga gaccactaat atgatgaacg aatcgcatta gatagcgacg catttccatt
     9841 gcagaatgcc acggttcaaa tgcaaacatc gttttccagt aaatccaaaa gtttgaatta
     9901 aagaagtcat cggaaaatac atctgttatt ttgacatcat ctaaatcttc ttcattcgtt
     9961 aagcataaat ctaaaatttc tttaatcgcc gttttagtca aagtgaagtc tccgtctgtg
    10021 actaaacgtt gaccctgttt ctcaataaca cgacagcgag aatagttagg gtcttctttg
    10081 tttagccaat agaactcatc taatacagac gcgttatcga tttctaatga agggatagat
    10141 ctgaataagt cccacaaaca ttcaaagtgg ttctccattt cacgaccacc gcggacaaca
    10201 tagcctttta aaggcatatt ttcaccatca agactaccac ctgctttagg taactcttct
    10261 aaaatatgaa tcttcgaacc ttccatttga ccatccctta ttaaaaaaca agctgcagca
    10321 agtgaagcta gaccagatcc gattaagtaa gcggatttgt tttctacatt ttcaggtttt
    10381 ttagggcgcg caaatgcttc ataatttcca taactgtaat acatatccac caagtccttt
    10441 cgtattagaa tacactcaga ctatacccct ttgtgaattg caaatattag tatttatatt
    10501 ggtaaatttg acgaaattag atcattttta tatttttgat ttttaaaatt ttatcactcg
    10561 aatgtttttg gattctgagg tgcgggactt ggaataatta tgtattttga aagaagttac
    10621 ttaaactaac gcctagtaga gtgataattc cgcctataat agcaagggtt gttggtagct
    10681 cgtctaataa cagataagat aataataaag aaacgatagg tgttaaataa agagacattg
    10741 ttgcatcaga gacaccaact gacttcacaa tataagcaag caaaacgtat ggaattatag
    10801 tagggaatat agctaaataa agtaccgata ctattgatgt aaaagtggcg ccgtgtatat
    10861 cgttgatgat ttcaggaata aaaataagca taaatggtga gcttgccatt attgtatata
    10921 gtgtgaaagc gatgaagccg tatttttcta tgtatttttt ctggaaagta aaatacaaac
    10981 tttcactaaa agatgcaagt aaaataataa aaacacctaa tacattaata gttgtgtaat
    11041 catctttact tattgaaata atggatattc ctataaatgc gacgagtgaa cttaaccaat
    11101 tccattttga aaaatgctct tttaaaaata tataagctaa agcactagaa aaaataggcg
    11161 ttgtagagac tagaattcca gatatacctg cactaatcaa agcttcacca aaatttaaag
    11221 ctgtgtgata tatcacaaat ccacaaaatc ctaaaataaa aataacaggg atatctctta
    11281 gttcaggggt aggcaatttc tttataatta cgaacggcaa gagaattatt gttgctaaaa
    11341 ttaaacgaaa tgccgacaat gattctgcac taaaatcatt taacgcaatc tttatcattg
    11401 gaaatgcaga tccccacaat atgatagtaa ataaatatga tagaaaagta gtgtcttgaa
    11461 gtttattcat taatatcatc actcctttaa ttatgtgttt ctatattaaa aaatatgatt
    11521 taaaatgagt acaaccaatt ttgaatggat ttacctatcc aattttaaaa gggagggaga
    11581 agatggctaa atataaagat attgctagtg acataagaga taaaataatc acaggggatt
    11641 ggttttatgg aatgaaaata ccttcacata ggcagttggc gatacagtac aacgtaaata
    11701 gagtaacgat tattaaaagt attgagttat tagaagctga aggatttatc tatactaaag
    11761 taggtagtgg aacatatgtt aatgactatt tgaatgaagc acatattaca aataagtggt
    11821 ctgaaatgat gttatggtcc tctcaacaaa gaagtcagta tacggtgcaa ttaattaata
    11881 aaattgagac agatgattcg tatatacata taagtaaagg tgaattgggt atatcgttaa
    11941 tgccacatat tcaattgaaa aaagccatgt ctaatacagc cagtcatatt gaagacttat
    12001 cttttggtta taataatggc tatggttata tcaagttaag agatattatc gttgaacgaa
    12061 tgtcaaagca aggtataaat gtaggtagag aaaatgtaat gatcacttca ggcgctttac
    12121 atgccattca acttttatct attgggtttt taggtcaaga tgccataata atttcgaata
    12181 caccatcata tattcactct acaaatgttt ttgagcaatt gaattttaga catattgatg
    12241 ttccttataa tcaaattaat gaaattgata ccatcattga tagatttatt aattttaaaa
    12301 ataaagcgat ttatatagaa cctaggttta ataacccgac aggtcgttct ttaacgaatg
    12361 agcaaaagaa aaatataatt acttatagcg aaagacataa tattcctatc attgaagatg
    12421 atatctttag agatattttc tttagcgatc caactccttc tatcaaaact tatgataaat
    12481 tgggaaaagt tatacatata agcagttttt caaaaacgat tgcaccagca ataagaatag
    12541 gttggattgt tgcttctgaa aaaataatag agcaattggc agatgtaaga atgcaaattg
    12601 actatggatc cagtattttg tcacaaatgg ttgtatatga gatgttgaaa aataagtctt
    12661 atgataaaca cttagtaaag ttaaggtatg ttttaaaaga taaacgagac tttatgttaa
    12721 acatcctcaa taatttattt aaggatatag cacattggga ggttccaagt ggaggttatt
    12781 ttgtatggtt agtctttaaa atagatatag atattaaata tttattttac gaattgttaa
    12841 gtaaagaaaa aatattaatc aatccgggtt acatttatgg cagtaaagaa aagagtataa
    12901 ggctatcttt tgcctttgaa tcaaatgaaa atattaagca tgcgctctat aaaatttata
    12961 catatgtgaa aaaggtttaa ttaaaacaat aattcgaatc attatgtggc atgttaaaca
    13021 gctaaatata agctatgcac atttaacaag cggatactcc tgacgcaatt atagtatcat
    13081 tacatattgt ttaatcgttt ggggtaatgt aattaaaagg taaaataggc agagtgttat
    13141 acataaaatg taaagaaaga ggcgatagaa tgtctaaaat caggtctttt acaatattaa
    13201 gtctacttat ttacttagct atgatgtgct atacagtagt gacctatcca aaattaccaa
    13261 ccaaagtgcc tattcattat aatttagcag gggatgctga taattttgct gataaatggg
    13321 tgctgctttt gattaatagc gcatttatag tgatttggct tatatttttc attgcaggta
    13381 gatactatga acgatttgcc aaatggtcac attataatca tacaccacgt gaaattcgag
    13441 cgattaaatt atttttaagt acgttaaatt tagagattat gagctatatg tctatcttca
    13501 cagtattaga aatttggcaa atacaacacc atcatcaatt ca
//
LOCUS       DAKTIO010000063        13423 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.063, whole genome shotgun sequence.
ACCESSION   DAKTIO010000063 DAKTIO010000000
VERSION     DAKTIO010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13423
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.063"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..258
                     /locus_tag="O0293_001371"
     CDS             <1..258
                     /locus_tag="O0293_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCY0280260.1"
                     /translation="VAIAKVIKAFKLKPDCHCTSKYLNNLIEQDHRHIKVRKTRYQSI
                     NTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEIRDMLAS"
     gene            390..1004
                     /locus_tag="O0293_001372"
     CDS             390..1004
                     /locus_tag="O0293_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280261.1"
                     /translation="MGNLDFDHEGYRKLLPLKDFKHFIKHSDSKTRNHFIGMAFNNKT
                     DVFSRNFSNKMINIFPKNEFYIHIQQFPENDSPSYMVYNTDITAFIQFRLVGRSENKY
                     LENIIRDYGVLIEVEALHSFKKGMGAMLVSQLEKLSDKLFIPIYLYDTNLKDELYYQD
                     LGFFDTTKKGNHGEPLLVYKPKNLETNKKEKPIKSLFKKVFNIN"
     gene            1073..2344
                     /locus_tag="O0293_001373"
     CDS             1073..2344
                     /locus_tag="O0293_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280262.1"
                     /translation="MDYTNQKRKYIIFGIVLAITLLIVGIAYYLISLNNAKGQVKEFE
                     KLVQHNDYNSIAKKLSNKEETLSENDAKNFVLFVKSKENQEQYNEQIQKIKSNIDKNK
                     DNNTVYGSITDQYGNNLITIKKNGKTFFFIDKLEFKPKLTSVYVKEFNNTGIYKYQQE
                     NAKKVIAESNQTTELGKFFIGKYSIKTDKTIEDSIHNGTLTGKIDIDLSNRGNNNKIY
                     ADEDFNQSWFKVKLNNDSLLEKNSYKLLIDDKDVDYNSKKTYGKYYNPTELSVYAIGK
                     LEGKEFKTNRINIRRNYDNKPQNLKLSFKESQIRDYESKSKKVKEKAKSYIKEYTKEL
                     NKAYKKKDYKYISDYVKNNSQLEKQLKKQVKSKSKVKFKDVKINNVKRDKNKIYIELS
                     KTNNGNVFQTKYTLDYDNVSNEFLVEDSHDN"
     gene            complement(2386..2802)
                     /locus_tag="O0293_001374"
     CDS             complement(2386..2802)
                     /locus_tag="O0293_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HCY0280263.1"
                     /translation="MKIINPNMPEPYKYETDYRNIPREYLNPHIPKGRGIVKWNAFKT
                     IPQQYEILEQHIEDQNKINMPLLSDDQLQEINEKVSSKISKNILTDLDYWKDGYIYSI
                     QCYINKIDEFNGFMIITNSNSNNKLKIPLSSIVSVQ"
     gene            3097..3654
                     /locus_tag="O0293_001375"
     CDS             3097..3654
                     /locus_tag="O0293_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCY0280264.1"
                     /translation="MANIGYARVSTRDQSLDLQIDALSNFGCEKIFSEKVSGRKVNRT
                     ELDKCLDYLREGDTLVIYKLDRLGRTTKQLIDLAQWLENNNIELQIIDMNINTKDPMG
                     KMFFTMMSAFAELEANLLSERTKKGLESARARGNMGGRKAMPQEKKEYIKHLYDTKEY
                     TGQEIAKKTNVSRDTVYRILREFKK"
     gene            3936..4715
                     /locus_tag="O0293_001376"
     CDS             3936..4715
                     /locus_tag="O0293_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280265.1"
                     /translation="MEVVRLNQNLFNKLRGNEISSNKNGSRPYYYSFKRNNNRVCIPF
                     RTNAQKVPNKYKINLGGEQPDKPNSAIDLTKSIVISNDEYLNNRSKAKIPQNVNNFLK
                     QQAPAIEQKYDTMSNDYIKAKASLSKIPLVKYSTMQYFHKELNIQDSIDNQQTKNAIN
                     ELISNGKSNKYNKLQSSLPNEKLNLLDDYETLYEFKSLTDYPAKINSNDIDNPFLEVE
                     KNNKHFTLSALTIKNEPEKHVKDFLNYDIENEKNKDIDLDL"
     gene            4849..6465
                     /locus_tag="O0293_001377"
     CDS             4849..6465
                     /locus_tag="O0293_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280266.1"
                     /translation="MRFTPRGILNNTDYELNSEFYYVPASDKQMNDQRDMTNKSIVGN
                     LVYRNNFYNEVYTQPVYDLDDSNEKYQTPHIISIDDSEFDYNLTNLDDYFTKDTANKG
                     YEKLVNAIKSEEITYFDSDINEVDDFTQQDEYSNDWYTNTFVDLVLSMKGYSYHVEPN
                     RESVIEDSHIITENDITYNVSYNPEKHHLYINDFPAIPIEENDNIRLLNDNLTQDMEK
                     TYDNINYNFSYNDFKTLEDDSYIYIDLTDYKDNEGKFVKNLIEDIPPNEIIKDRLKQN
                     INLFDERANYNALAVYRRDLEDFSDRNNLDSLDKVKDLKTFYDTEINIKGLNINNDIE
                     KKALNNQLAFNLYNEPLNNMFRDNNVLYEVYEKEIYDEKIPLSIEDRKEVKTYLDDAR
                     NNFSNNFYEYTTNKSSILAENSLTVLDKAKYDLEFAPVSNHLYKERENFYEGMKQATL
                     PLHNITKEQYKERENDIEIIGHFKNYINTELHGTKSSEFKPNFVNDSLENETNSIAFE
                     DIINGSEETHSNIINSIKNELKQERDNLEM"
     gene            6476..6664
                     /locus_tag="O0293_001378"
     CDS             6476..6664
                     /locus_tag="O0293_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000921494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280267.1"
                     /translation="MLKTYTEIIGSELPIYITKSETSELLFSEELDKNLKYEVAGYYL
                     YNAKKDLYKELYKPISEV"
     gene            6668..8392
                     /locus_tag="O0293_001379"
     CDS             6668..8392
                     /locus_tag="O0293_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LtrC"
                     /protein_id="HCY0280268.1"
                     /translation="MAYSKGNYKKKKIDSIIEELNNKLDDFKNNDKTYKEFLDTTAKF
                     HNYSINNIILIAGQNPEATAVAGYKAWKNKFDRQVQKGAKSMNIIAPIVQKRNVEIKD
                     DNGNIVRDNNGNPKTERKPVITGYRAHNVFDVADTKGKPLITAKDLIKTEFENSNDYK
                     DLYNEFKDYINEELTPSVEEKHFLDDPTLSNGAKGYYSPKSDEIVISDDLSYDMRFKT
                     LIHEYAHSQLHNNDIGKTQISEHSRSLKEIEAESSAYVVANYYGLDTSDYSLGYLSGW
                     GHNISDDELKAHIKNIHSFAKTTIEEINSLPEFSQYIDKKLESEMNKDVYKDLSTMID
                     TNLKNGFDKITIIKGNLVNDYGLNEISENSYENDDFKVNIDYKGFNTNNSQDQAKIEL
                     INKHDDSLNRDYNFTQTYNRNVINNTTTIFVQDDDNEDKNKKYIHERDIDGNILEEKH
                     KLSPQDEIVSFENFVNKSVEENGLKDTLRQFVSNGVLMGYELNVDNIDNTNETYFSMN
                     KNQQNGQRSNMTAKLEHDTNENVYIDFKIKNSAGIKNISYTESNEEFNKDEQNKEIEN
                     KQEEEIDI"
     gene            complement(8433..10430)
                     /locus_tag="O0293_001380"
     CDS             complement(8433..10430)
                     /locus_tag="O0293_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HCY0280269.1"
                     /translation="MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSN
                     KQCDYSEILLPNNADDKFKDREYLWNKVHDVENRKNSQVAREIIIGLPNEFDPNSNIE
                     LAKEFAESLSNEGMIVDLNIHKINEENPHAHLLCTLRGLDKNNEFEPKRKGNDYIRDW
                     NTKEKHNEWRKRWENVQNKHLEKNGFSVRVSADSYKNQNIDLEPTKKEGWKARKFEDE
                     TGKKSSISKHNESIKKRNQQKIDKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDL
                     KIYVTPINMYKENERLYDLKQKTSLITDDEDRLNKIEDIEDRQKKLESINEVFEKQAG
                     IFFDKNYPDQSLNYSDDEKIFITRTILNDRDVLPANNELEDIVKEKRIKEAQISLNTV
                     LGNRDISLESIEKESNFFADKLSNILEKNNLSFDDVLENKHEGMEDSLKIDYYTNKLE
                     VFRNAENILEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQQLIDFKTYHGTENTIEML
                     ETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLSEYDFDYSD
                     NNDLKHLYQESNEVGDEISKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLINAGLDS
                     MIFNFNEIFRERMPKYINHQYKGKNHSKQRHELKNKRGMHL"
     gene            10634..10906
                     /locus_tag="O0293_001381"
     CDS             10634..10906
                     /locus_tag="O0293_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280270.1"
                     /translation="MADIQKMLEQKEKLQEKIKEEKKKEYEKFGRWFFNKFKVNRSAD
                     AKKIINKNLLNIEDTKEHNLERNEIIEETKNSNEENKEINEINHNI"
     gene            11010..11243
                     /locus_tag="O0293_001382"
     CDS             11010..11243
                     /locus_tag="O0293_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280271.1"
                     /translation="MILLSTAGENAVHINPIFRFFLYFIMCVPMTVALCTIFLKFISI
                     FILPWKVCLYIGIALGIIVTIFLLVITIGIEMT"
     gene            11506..12546
                     /locus_tag="O0293_001383"
     CDS             11506..12546
                     /locus_tag="O0293_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="parM protein"
                     /protein_id="HCY0280272.1"
                     /translation="MSNVYVMALDFGNGFVKGKINDEKFVIPSRIGRKTNENNQLKGF
                     VDNKLDVSEFIINGNNDEVLLFGNDLDKTTNTGKDTASTNDRYDIKSFKDLVECSIGL
                     LAREVPEEVVNVVIATGMPSNEIGTDKQAKFEKLLNKSRLIEIDGIAKTINVKGVKIV
                     AQPMGTLLDLNMENGKVFKAFTEGKYSVLDFGSGTTIIDTYQNMKRVEEESFVINKGT
                     IDFYKRIASHVSKKSEGASITPRMIEKGLEYKQCKLNQKTVIDFKDEFYKEQDSLIEE
                     VMSNFEITVGNINSIDRIIVTGGGANIHFDSLSHYYSDVFEKADDSQFSNVRGYEKLG
                     ELLKNKVEQESK"
     gene            12549..12878
                     /locus_tag="O0293_001384"
     CDS             12549..12878
                     /locus_tag="O0293_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParR"
                     /protein_id="HCY0280273.1"
                     /translation="MDKKETKHLLKIKKEDYPQIFDFLENVPRGTKTAHIREALRRYI
                     EEIGENPPIPSKEHTAPLKEHTEPIKRNKESFKDAMERKDQLKQAEMPKIEEKDEEMV
                     DISIDDL"
     gene            complement(12882..13142)
                     /locus_tag="O0293_001385"
     CDS             complement(12882..13142)
                     /locus_tag="O0293_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCY0280274.1"
                     /translation="MNEYRIQKLYRYICLEFKNQRQLIGKRQEEVAFDLSVTAGHLSR
                     IENGKKPRIALHTFLVMSEYYGVDFHKVVKNAEEKMELDEGI"
BASE COUNT         5369 a         1689 c         1915 g         4450 t
ORIGIN      
        1 gtagcaatag ctaaagtaat taaagctttt aaacttaaac ctgactgtca ttgtacatcg
       61 aaatatctga ataacctcat tgagcaagat caccgtcata ttaaagtaag aaagacaaga
      121 tatcaaagta tcaatacggc aaaaaataca ctcaaaggca ttgaatgtat ttacgctcta
      181 tataaaaaga accgcagatc tcttcagatc tacggatttt cgccatgcca cgaaattagg
      241 gatatgttag ccagttaagg gaacaacgac atgataaaat ggtagttagc tatatttttc
      301 taactttgca acagaaccaa ataaaagata aaataacatc ttggcttgat aaagataaca
      361 acaaaaacga ataatatagg aggggttttt tggggaattt agattttgat cacgaaggat
      421 acagaaaact attaccatta aaagatttca aacattttat aaaacatagc gacagcaaga
      481 ctagaaatca ttttattggt atggctttta ataataaaac agatgtgttt agtcgtaact
      541 tctctaataa aatgataaac atattcccta aaaatgaatt ttatattcat atacaacaat
      601 ttccagagaa tgatagccct tcttatatgg tatacaacac tgatattaca gcatttattc
      661 agtttaggtt agttgggcga tcagaaaata agtatttaga aaacattatc agagattatg
      721 gcgtattaat agaagttgag gccttacatt catttaaaaa aggtatgggg gcaatgcttg
      781 taagtcaatt ggaaaagtta tcagataaac tgtttatacc tatatatctt tatgatacta
      841 atttaaaaga tgaattatat tatcaagact taggattctt tgatactact aaaaaaggga
      901 atcatggaga accactttta gtatataaac ctaaaaatct agaaactaat aaaaaagaaa
      961 agcctattaa atctttattc aagaaagtat tcaatatcaa ttagcctaca gtattaaaga
     1021 ggtggggtaa ctcatttctt taatacttat tttattaaca ggggtttaat atatggatta
     1081 cacaaatcaa aaaagaaaat acataatatt tggtattgtt cttgctataa cacttctaat
     1141 tgttggaatt gcttactatt taatcagttt gaataatgct aaaggacaag ttaaagaatt
     1201 tgaaaaatta gtacagcata atgactacaa tagtattgct aaaaagttat ctaataaaga
     1261 agaaacttta tctgaaaatg atgctaaaaa ctttgtgtta tttgttaaaa gtaaagaaaa
     1321 tcaagaacaa tacaatgagc aaatacaaaa aattaaaagt aatattgata aaaataaaga
     1381 caataatacc gtgtatggct ctataacaga tcaatacgga aataacctaa taactattaa
     1441 gaaaaacgga aagactttct tttttataga taaattagaa ttcaaaccta aacttaccag
     1501 cgtttatgtg aaagaattta ataacactgg aatttacaaa tatcagcaag aaaatgctaa
     1561 aaaggtaata gcagaaagca atcaaacaac agaattaggc aagttcttta ttggtaaata
     1621 tagtattaaa acagataaaa caattgaaga ttcaattcac aatgggactt tgacaggaaa
     1681 aatagatata gatttaagca atagaggaaa caataataag atatatgcag atgaagattt
     1741 taaccaaagc tggtttaagg ttaagctaaa caatgatagt ttattagaaa aaaatagtta
     1801 caaactttta attgatgata aagatgttga ttataattcc aaaaagacat atggaaagta
     1861 ttacaatcca actgaattaa gtgtatatgc gattggaaaa ttagagggta aagagtttaa
     1921 aactaacaga ataaatataa gacgtaatta tgataataaa cctcaaaatt taaaattaag
     1981 ttttaaggaa tctcaaataa gagattatga gagtaaaagt aaaaaggtta aagaaaaagc
     2041 taaaagctat attaaagaat atacgaaaga gttaaacaaa gcatataaaa agaaagatta
     2101 taaatatata tctgactacg ttaaaaataa ctctcaatta gaaaaacaac taaaaaaaca
     2161 ggttaagtct aaatcaaaag ttaaatttaa agatgttaag ataaacaacg ttaaacgtga
     2221 taaaaacaaa atatatattg agttaagtaa aaccaacaat ggtaatgtgt ttcaaacgaa
     2281 atatacctta gattacgata atgtatcaaa tgaatttcta gtagaagatt cacatgataa
     2341 ttaaaataat tcaaacacca atgacattaa attcattggt gttttttact gtacgctaac
     2401 aatactactt aaaggtattt ttagtttatt gtttgaattg gaattagtga taatcataaa
     2461 tccattaaat tcatctattt tgttaatgta acattgaatc gaatatatat atccgtcttt
     2521 ccagtaatct aaatcagtta atatattttt actaatctta ctacttactt tctcattaat
     2581 ttcttgtagc tggtcatcag acaatagagg catatttatt ttattttgat cttctatgtg
     2641 ttgttccaat atttcatatt gctggggtat cgtcttaaat gcgttccatt taactattcc
     2701 tctacctttt ggaatatgtg gatttaagta ttccctaggt atgtttctat aatcagtttc
     2761 atatttgtac ggttcgggca tattaggatt tattattttc atatcagtaa acacctcaac
     2821 caaattatag aacatatgtt cgtaaaaata aagagtgaaa taagagtatt ataaacgatt
     2881 gtatttatta cactgataat tacttttctt aatagcgtta taatgggttg actggtgtat
     2941 taaaatcttg tattataatc atagtaagat aattaacaaa taacatggca aataatacaa
     3001 atagattagc tctcatttgc attgagaatt aattttatag ataactatac attaaaaaca
     3061 atatacaacg ctattttaga aaggataatt acaattatgg ccaatattgg ttatgctcgt
     3121 gtatctacca gagatcaaag tttagattta caaatagacg ctttgagtaa ttttggttgt
     3181 gagaaaatat ttagcgaaaa agttagtggg cgtaaagtaa atagaactga actagataaa
     3241 tgtttagatt atttacgtga aggcgacact ttagttattt acaagctaga tagattaggc
     3301 aggactacta agcaactgat agatttagcc caatggttag aaaataataa tattgaatta
     3361 caaattattg atatgaatat caatacaaaa gacccaatgg gaaagatgtt ttttacaatg
     3421 atgagtgctt ttgctgaatt agaggctaac ttattaagcg aaagaactaa aaaaggttta
     3481 gaatcagcta gagcaagagg taacatggga ggcagaaaag caatgccaca agaaaaaaaa
     3541 gaatacatta aacatttata cgataccaaa gaatatacag gacaagaaat tgctaaaaaa
     3601 acgaatgtaa gtagagatac tgtatatcgc attttacgag agtttaaaaa gtaatcctaa
     3661 acattgcata acatgacagg tattgatata atataagtaa gaaaagaatc ggcgttgtgg
     3721 cgcagatata aaaattaata ctacacgaac tagaatcggc gttgtggcgc agatataaaa
     3781 attaatactg cacgaactag aatcggcgtt gtggcgcaga tataaaaatt aatactgcac
     3841 ggaaagaaac aactatagag atcaaaacac attatattgt tttaaggagg cgtatattac
     3901 gcctcttttt tattaaattt atggagtgaa taacaatgga agttgttcgt ttaaatcaaa
     3961 acctatttaa taaactacga ggaaacgaaa tatcaagcaa taaaaatggt tcaagaccat
     4021 attattattc tttcaaacgt aataataata gagtttgtat accttttaga acaaatgcac
     4081 aaaaggtacc taataaatat aaaattaatt taggaggcga acagcccgat aaacctaatt
     4141 cagctattga tttaacaaag tctatagtaa ttagtaatga tgaatatctt aataacaggt
     4201 caaaagctaa aataccacaa aatgttaata attttttaaa gcaacaagca ccagctattg
     4261 aacaaaaata cgatactatg tctaatgatt acataaaagc aaaagctagt ttaagtaaga
     4321 tacctttagt taaatattca actatgcaat acttccacaa agaattgaat attcaagata
     4381 gtattgataa tcaacaaact aaaaatgcta ttaatgaatt aatatctaat gggaaatcaa
     4441 ataaatataa taaattacaa tcttctctgc ctaatgaaaa attaaatttg ctagatgatt
     4501 atgaaactct atatgagttt aaaagcctaa cagactaccc agctaaaata aattctaatg
     4561 atattgataa tcctttctta gaagttgaga aaaataacaa acattttact ttgtctgcac
     4621 taactattaa aaatgaacca gaaaagcatg taaaagactt tttaaattat gatatagaga
     4681 atgaaaagaa taaagatatt gatttagatt tataaggcct gtaaaaaagg ccttttttct
     4741 ttttatttat tatgacacga catgaaatga cacgtcacaa atggtataat tactatatgg
     4801 aggttatatt tacattaggt ttttattaaa aaaaggagtg atttaataat gagatttaca
     4861 ccccgaggaa ttttgaacaa caccgattat gaactaaatt ctgaatttta ttatgtgcca
     4921 gcaagtgata aacaaatgaa tgatcaaaga gatatgacta ataaaagcat agtaggtaat
     4981 ttagtctata gaaataattt ctataacgaa gtgtatactc aaccagttta tgatttagac
     5041 gattctaacg aaaaatatca aacaccacat attataagta tagatgattc tgaatttgat
     5101 tataacttaa ctaatttaga tgattatttt actaaagata cagcaaataa aggttatgaa
     5161 aagctagtaa atgccattaa atcagaagaa attacctatt ttgacagtga tattaatgaa
     5221 gttgatgatt ttacacaaca agatgaatat tcaaatgatt ggtatactaa cacttttgtg
     5281 gatttagtat taagtatgaa aggatattca tatcatgtag aaccaaatag agaatctgtt
     5341 attgaggatt cacatatcat aacagaaaat gatattactt ataatgtaag ctataacccc
     5401 gaaaaacatc atttatatat taatgacttc ccagcaattc caatagaaga aaacgataat
     5461 ataagattat taaatgataa cttaactcaa gacatggaaa aaacttacga taatataaac
     5521 tataattttt catacaatga ttttaaaaca cttgaagatg attcttatat ttatattgat
     5581 ttaacagatt ataaagataa cgaaggtaaa tttgttaaaa atctaataga agatatacca
     5641 cctaatgaaa ttattaaaga tagattaaaa caaaatatta atttatttga cgaacgagca
     5701 aattataatg ccttagctgt ttatagaagg gatttagaag atttttcaga tagaaataat
     5761 ttagatagct tagataaagt taaagattta aaaacattct atgatacgga aattaatatt
     5821 aaaggattga acattaacaa cgatattgag aaaaaggctt taaataatca attagccttt
     5881 aacctttata atgaaccgtt aaataacatg tttagagata acaatgtttt atatgaagtt
     5941 tatgaaaaag aaatttatga tgaaaaaatt cccctttcta tagaagatag aaaagaagtg
     6001 aaaacttatt tagatgacgc tagaaacaat ttttcaaata atttctatga atatacaacg
     6061 aacaaatcat ctattttagc tgaaaatagt ttaactgttt tagataaagc taaatatgat
     6121 ttagaatttg cccctgtatc taatcattta tacaaagaaa gagaaaattt ttacgaagga
     6181 atgaaacaag ctaccctgcc cttacacaat attactaaag aacaatataa agaaagagaa
     6241 aatgatattg aaataatcgg tcattttaaa aactatatca atactgaatt acatggaaca
     6301 aagagttctg aattcaaacc taatttcgta aatgattctt tagaaaatga aactaactca
     6361 atagcttttg aagatattat aaatggatca gaagaaacac atagtaatat tattaattct
     6421 attaaaaatg aattaaagca agaaagagat aatttagaaa tgtaggaggt acaatatgct
     6481 aaaaacatat actgaaatca ttggaagtga attgccaatt tatataacaa aatcagaaac
     6541 aagcgaatta ctattttcag aagaattaga taaaaattta aaatatgaag ttgctggata
     6601 ttatctatat aatgctaaaa aagacttata caaagaactt tataaaccta ttagcgaggt
     6661 gtagtatatg gcttattcaa aaggaaatta taaaaagaaa aaaatagatt caataataga
     6721 agaattgaat aataagctag atgacttcaa aaacaatgac aaaacctata aggaattttt
     6781 agatactaca gcgaaatttc acaactattc aattaataat attattctaa ttgctggcca
     6841 aaatccagag gctacagctg tagctggtta taaagcatgg aaaaataaat ttgatagaca
     6901 agtgcaaaaa ggtgcaaaaa gtatgaatat aattgcccct attgtacaaa aacgaaatgt
     6961 agaaattaaa gatgataacg gtaatatagt acgtgataat aacggtaatc ctaaaactga
     7021 acgaaaaccc gtaattactg gatatagagc gcataatgtg tttgatgtgg cagatacgaa
     7081 aggcaagcct ttaataacag caaaagacct aataaaaacc gaatttgaaa actctaacga
     7141 ctataaagac ttatataatg aatttaaaga ttatattaat gaagaattaa cacctagtgt
     7201 agaggaaaaa cattttcttg atgatcctac tttatctaat ggcgctaaag gttattatag
     7261 tcctaaatct gatgaaattg taatctctga tgatttgtca tatgatatgc gctttaaaac
     7321 tttaatacat gaatatgcac actctcaatt acacaataat gatataggta aaacgcaaat
     7381 atctgaacat agtagatcat taaaagaaat cgaggctgaa agttctgctt atgtagtagc
     7441 taattattac ggtttagaca ctagcgatta ttctttaggt tatttaagtg gctgggggca
     7501 taatataagt gatgatgaat taaaagccca cataaaaaat atacattcgt tcgcaaaaac
     7561 tacaattgaa gaaataaatt cattgccaga gttttctcaa tatatagata aaaaattaga
     7621 atcagaaatg aacaaagatg tatataaaga cctttcaaca atgatagata ccaatctaaa
     7681 aaatggtttt gataaaataa ctattatcaa aggtaattta gttaatgatt atggacttaa
     7741 tgaaatttct gagaatagtt atgaaaatga tgattttaaa gtgaatattg attacaaagg
     7801 atttaatact aataattcgc aagatcaagc taaaattgag ttaattaata agcatgatga
     7861 ttcattgaat agagattaca acttcacgca aacttataac cgaaatgtaa ttaataacac
     7921 cacaactatt tttgtacaag atgatgataa tgaagataaa aacaaaaaat atatccatga
     7981 acgagatata gacggtaata tattagaaga aaaacataaa ttaagcccac aagatgaaat
     8041 agtatcattt gaaaactttg taaataaatc agttgaagaa aatggcctaa aagatacatt
     8101 acgccaattt gtaagtaatg gtgtattaat gggctatgaa ttaaatgttg ataatattga
     8161 taatacaaat gaaacttact tttcaatgaa taaaaatcaa caaaatggcc aacgatcaaa
     8221 tatgactgct aaattagaac atgatactaa cgaaaatgtt tatatagatt ttaaaattaa
     8281 aaattcagct ggtattaaaa acatatccta tactgaaagt aatgaagaat ttaataaaga
     8341 tgaacagaat aaagaaattg aaaacaaaca agaagaagaa attgatatat aaaaagaagg
     8401 catatacgcc ttcttttttt atacaatact atttataagt gcatacctct tttatttttc
     8461 aactcatggc gctgtttact gtgattctta cctttgtact gatgatttat atacttcggc
     8521 atacgttctc taaatatctc attaaaatta aatatcattg aatctagtcc agcatttatt
     8581 aaaccatagg cttgttgttt tctattgtaa ttattgtaag tttctttatc tactagaata
     8641 tcattacctt cataaaactc ttcaatgtta tctttgctta tttcgtcccc tacttcattg
     8701 gattcttgat ataaatgttt taaatcattg ttatcactat aatcaaagtc atattcactc
     8761 aataatttct tttgaatagc cccaaataca aatttatcat gctgattttt attaggttta
     8821 aattcttttt cttgtaacaa cttaacttgt tcagttatat attttctatc ttcatcactg
     8881 tatttaggaa taaaattacc tgtttctaac atttcaattg tgttttctgt accatgataa
     8941 gttttaaaat caattaattg ttgtttttct ttaatgtcag ttacttcatt aaaagcttta
     9001 taatcttcat catctgtaaa taattccttt atttgtacat cataataatc ttctagtata
     9061 ttttcagcat ttctaaaaac ttctaatttg ttagtataat aatctatttt caaactatct
     9121 tccatacctt catgtttatt ctctaacaca tcatcaaaag acaagttatt tttttcgaga
     9181 atatttgaaa gtttatcggc aaagaaattc gattcttttt caatagattc taaacttata
     9241 tctctgttac ctaaaactgt attaaggctt atttgagcct cttttatgcg tttctcttta
     9301 actatatcct ctagttcatt attagctggt aaaacatctc tatcatttaa tatagtgcgt
     9361 gtgataaaga ttttttcatc atcactatag tttaatgatt gatcgggata atttttatca
     9421 aagaaaattc cagcttgttt ttcaaatacc tcatttatag attcaagttt tttctgtcta
     9481 tcctctatat cctcaatttt atttaatcta tcttcatcat cagtgattaa tgaagttttt
     9541 tgttttaaat catatagtct ttcattttcc ttatacatat ttataggtgt tacataaatt
     9601 tttaaatctt tggccatatt ttttaatgta gtagaatctg atttattcat ataactaaaa
     9661 gcatttaatt tgtgagattt catatcatct acttcattaa acatcttatc aattttttgc
     9721 tgatttctct ttttaatact ttcattatgt ttcgatattg aagatttttt tcctgtttca
     9781 tcttcaaatt ttcgtgcttt ccaaccttct ttttttgttg gctctaaatc tatattttga
     9841 tttttatatg aatcagcaga aactctaaca gaaaaaccat tcttttctag gtgtttattt
     9901 tgaacatttt cccaccgttt tcgccattca ttatgttttt cttttgtatt ccaatctcta
     9961 atataatcat tgccctttcg cttaggttca aattcattat ttttatctaa accacgtaat
    10021 gtacataata aatgtgcgtg aggattttct tcatttattt tatgaatatt tagatcaaca
    10081 atcattcctt cattagataa ggattcagca aattcttttg ctaattcaat attactattt
    10141 ggatcaaatt cattcggcaa accaattata atttctcgtg caacttgact attttttctg
    10201 ttctcaacat catgaacttt attccacaaa tattctctat ctttaaactt atcatcagca
    10261 ttattaggta ataaaatttc actataatca cattgcttat ttgagtaatc tttaaattcg
    10321 ttttccttaa aatctttaat cctactagcg ctattatagg cactttttgc agttgcactt
    10381 tgtccatttg cttttgatac aaacttattt tgaaaatggt acattgccat aataaaatct
    10441 ccttaaaaaa caaatattga gcgaaggcga aataaattaa aaattaattt ttaatttcac
    10501 gaatttagca cgcgaacgga acgttcgcat aagtgcgccc ttacgggatt taactagatt
    10561 ataacggtaa aacttgaatc tgtcaaacga aaaggttata attaaattaa taaaaaatat
    10621 ggaggtttta tttatggctg atattcaaaa aatgttagaa caaaaagaaa aacttcaaga
    10681 aaaaattaaa gaagaaaaga aaaaagaata tgaaaaattt ggaagatggt tcttcaataa
    10741 atttaaagta aatagatcag cagacgcaaa gaaaattata aataaaaatt tgcttaatat
    10801 tgaagatact aaagaacaca atttagaaag aaatgaaatt atagaagaaa ctaaaaattc
    10861 caatgaagaa aacaaagaaa taaatgaaat aaaccacaat atttaataga tattaactaa
    10921 tattaatatg atgaaagact tataaaataa ttggtttact aatagcaaaa tctacagtag
    10981 tcaatacttt aaaaatagga ggttttatta tgattttatt atcaacagct ggcgaaaatg
    11041 ctgttcatat taaccctatt tttagatttt ttctatattt tattatgtgt gttcctatga
    11101 cagttgcttt atgtacaata ttccttaaat ttatctcaat ttttattctt ccatggaaag
    11161 tatgtttgta tattggtatt gctttaggta ttattgttac tatttttctt ttggttataa
    11221 caattggtat agaaatgaca taaattttct acgaaaatat caaacatgac atgacatgta
    11281 ataaagtatg tgttaaaatt cattataaga agtagcaatc aagtaaataa atgctggttg
    11341 ttattttttt acccacacag tatatactag tatatactaa gatatactat aatatatact
    11401 gatatatact aggatatatc agtatagaaa agtatataaa taacttgtgg tattttatta
    11461 attgtactaa tttaaaaaga gtaaataata tgtggaggtt ttattatgag taacgtatat
    11521 gtaatggctt tagattttgg taatggattt gtaaaaggga agattaatga tgaaaaattt
    11581 gtaatccctt caagaatcgg aagaaaaact aatgaaaaca atcaattaaa aggatttgtt
    11641 gataataaat tagatgtatc agaatttatt atcaatggga acaatgatga agtattacta
    11701 tttggtaatg atttagataa aactacaaat actggtaaag atactgcctc aacaaatgat
    11761 agatatgata ttaaatcttt caaagatttg gttgaatgtt ctataggatt attagcaaga
    11821 gaagtaccag aagaagttgt caacgttgta attgctactg gtatgccaag taatgaaata
    11881 ggaactgata aacaagctaa gtttgaaaaa cttttaaata aaagtcgttt aattgaaatt
    11941 gacggtatcg ctaaaacaat taatgttaaa ggcgttaaga tagtagctca acctatggga
    12001 actttacttg atcttaatat ggaaaatgga aaagtattca aagcatttac agaaggcaag
    12061 tacagtgttc ttgactttgg tagtggtact acaattattg atacttatca aaatatgaaa
    12121 cgtgtagagg aagaaagttt tgttataaac aaaggtacta ttgattttta taaacgaatt
    12181 gctagtcacg tatctaaaaa atcagaaggt gcctcaatca caccacgtat gattgaaaaa
    12241 ggattagaat acaagcaatg taaacttaat caaaaaacag ttattgactt taaagatgaa
    12301 ttttataagg aacaggatag tttgatagaa gaagttatga gtaactttga aatcactgta
    12361 ggtaatataa attcaattga cagaattatt gttactggtg gtggtgcaaa cattcatttt
    12421 gattcattaa gtcattatta ttcagatgtg ttcgagaaag cagatgatag ccagtttagt
    12481 aatgttcgtg gatatgaaaa attgggggaa ttgttaaaaa acaaagtaga gcaagaaagt
    12541 aaataattat ggataagaaa gaaactaaac atcttctgaa aataaaaaaa gaagattacc
    12601 ctcaaatctt cgatttttta gaaaatgttc ctagaggtac taaaacagcc cacataagag
    12661 aggcattgag gcgctatatt gaagaaatag gggaaaaccc tccaatacct tcaaaagaac
    12721 acacagcgcc cttaaaagag catacagaac ctattaaacg taataaagaa agttttaaag
    12781 acgcaatgga acgtaaggat caattaaaac aagctgaaat gcctaaaata gaagaaaaag
    12841 atgaagaaat ggtagatatt agtattgatg acctttaata tctatattcc ttcatccaat
    12901 tccatttttt cttctgcatt tttaactacc ttatgaaagt ctacaccata gtattcagac
    12961 attactaaaa atgtatgtaa tgctattcta ggttttttac cattctcgat acgtgaaaga
    13021 tgaccagcag tcacactcaa atcaaatgca acttcttctt gtcgcttgcc tatgagttga
    13081 cgctggtttt taaattctaa acaaatgtat ctatatagtt tttgtatacg gtattcattc
    13141 atgatttatc ggcctcataa ttttagatat attaattata ttagagattt gttatcaaat
    13201 acatttcatt acaagcatat aattgacctg tgaggcaaca aaaaattttt aattttttta
    13261 aaatagtgtt actattgaac atagaaaaag acacctaacg cttggcggtt gttagatgtc
    13321 taaagagttt aaataatatg tgttatttac ttttacaatt tgtttgtatc tttattatat
    13381 atacaacctc gatacattac aagataaaag caacaagtct tta
//
LOCUS       DAKTIO010000064        13148 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.064, whole genome shotgun sequence.
ACCESSION   DAKTIO010000064 DAKTIO010000000
VERSION     DAKTIO010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13148
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.064"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            108..1394
                     /gene="rseP"
                     /locus_tag="O0293_001386"
     CDS             108..1394
                     /gene="rseP"
                     /locus_tag="O0293_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HCY0280275.1"
                     /translation="MSYLVTIIAFIIVFGVLVTVHEYGHMFFAKRAGIMCPEFAIGMG
                     PKIFSFRKNETLYTIRLLPVGGYVRMAGDGLEEPPVEPGMNVKIKLNEENEITHIILD
                     DHHKFQQIEAIEVKKCDFKDDLFIEGITAYDNERHHFKIARKSFFVENGSLVQIAPRD
                     RQFAHKKPWPKFLTLFAGPLFNFILALVLFIGLAYYQGTPTSTVEQVADKYPAQQAGL
                     QKGDKIVQIGKYKISEFDDVDKALDKVKDNKTTVKFERDGKTKSVELTPKKTEKKLTK
                     VSSETKYVLGFQPASEHTLFKPIVFGFKSFLIGSTYIFTAVVGMLASIFTGGFSFDML
                     NGPVGIYHNVDSVVKAGIISLIGYTALLSVNLGIMNLIPIPALDGGRILFVIYEAIFR
                     KPVNKKAETTIIAIGAIFMVVIMILVTWNDIRRYFL"
     gene            1414..3117
                     /locus_tag="O0293_001387"
     CDS             1414..3117
                     /locus_tag="O0293_001387"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCY0280276.1"
                     /translation="MKQSKVFIPTMRDVPSEAEAQSHRLLLKSGLIKQSTSGIYSYLP
                     LATRVLNNITAIVRQEMERIDSVEILMPALQQAELWEESGRWGAYGPELMRLQDRHGR
                     QFALGPTHEELVTSIVRNELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDA
                     YSFHADEASLDQTYQDMYQAYSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGE
                     DTIVYSKESDYAANIEKAEVVYEPNHKHTTVQPLEKIETPNVKTAQELADFLGRPVDE
                     IVKTMIFKVDGEYIMVLVRGHHEINDIKLKSYFGTDNIELATQDEIVNLVGANPGSLG
                     PVIDKEIKIYADNFVQDLNNLVVGANEDGYHLINVNVGRDFNVDEYGDFRFILEGEKL
                     SDGSGVAHFAEGIEVGQVFKLGTKYSESMNATFLDNQGKAQSLIMGCYGIGISRTLSA
                     IVEQNHDDNGIVWPKSVTPFDLHLISINPKKDDQRELADALYAEFNTKFDVLYDDRQE
                     RAGVKFNDADLIGLPLRIVVGKRASEGIVEVKERLTGDSEEVHIDDLMTVITNKYDNL
                     K"
     gene            3381..7691
                     /locus_tag="O0293_001388"
     CDS             3381..7691
                     /locus_tag="O0293_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCY0280277.1"
                     /translation="MTEQQKFKVLADQIKISNQLDAEILNSGELTRIDVSNKNRTWEF
                     HITLPQFLAHEDYLLFINAIEQEFKDIANVTCRFTVTNGTNQDEHAIKYFGHCIDQTA
                     LSPKVKGQLKQKKLIMSGKVLKVMVSNDIERNHFDKACNGSLIKAFRNCGFDIDKIIF
                     ETNDNDQEQNLASLEAHIQEEDEQSARLATEKLEKMKAEKAKQQDNNESAVDKCQIGK
                     PIQIENIKPIESIIEEEFKVAIEGVIFDINLKELKSGRHIVEIKVTDYTDSLVLKMFT
                     RKNKDDLEHFKALSVGKWVRAQGRIEEDTFIRDLVMMMSDIEEIKKATKKDKAEEKRV
                     EFHLHTAMSQMDGIPNIGAYVKQAADWGHPAIAVTDHNVVQAFPDAHAAAEKHGIKMI
                     YGMEGMLVDDGVPIAYKPQDVVLKDATYVVFDVETTGLSNQYDKIIELAAVKVHNGEI
                     IDKFERFSNPHERLSETIINLTHITDDMLVDAPEIEEVLTEFKEWVGDAIFVAHNASF
                     DMGFIDTGYERLGFGPSTNGVIDTLELSRTINTEYGKHGLNFLAKKYGVELTQHHRAI
                     YDTEATAYIFIKMVQQMKELGVLNHNEINKKLSNEDAYKRARPSHVTLIVQNQQGLKN
                     LFKIVSASLVKYFYRTPRIPRSLLDEYREGLLVGTACDEGELFTAVMQKDQSQVEKIA
                     KYYDFIEIQPPALYQDLIDRELIRDTETLHEIYQRLIHAGDTAGIPVIATGNAHYLFE
                     HDGIARKILIASQPGNPLNRSTLPEAHFRTTDEMLNEFHFLGEEKAHEIVVKNTNELA
                     DRIERVVPIKDELYTPRMEGANEEIRELSYANARKLYGEDLPQIVIDRLEKELKSIIG
                     NGFAVIYLISQRLVKKSLDDGYLVGSRGSVGSSFVATMTEITEVNPLPPHYICPNCKT
                     SEFFNDGSVGSGFDLPDKTCETCGAPLIKEGQDIPFETFLGFKGDKVPDIDLNFSGEY
                     QPNAHNYTKVLFGEDKVFRAGTIGTVAEKTAFGYVKGYLNDQGIHKRGAEIDRLVKGC
                     TGVKRTTGQHPGGIIVVPDYMDIYDFTPIQYPADDQNSAWMTTHFDFHSIHDNVLKLD
                     ILGHDDPTMIRMLQDLSGIDPKTIPVDDKEVMQIFSTPESLGVTEDEILCKTGTFGVP
                     EFGTGFVRQMLEDTKPTTFSELVQISGLSHGTDVWLGNAQELIKTGICDLSSVIGCRD
                     DIMVYLMYAGLEPSMAFKIMESVRKGKGLTEEMIETMKENEVPDWYLDSCLKIKYMFP
                     KAHAAAYVLMAVRIAYFKVHHPLYYYASYFTIRASDFDLITMIKDKTSIRNTVKDMYS
                     RYMDLGKKEKDVLTVLEIMNEMAHRGYRMQPISLEKSQAFEFIIEGDTLIPPFISVPG
                     LGENVAKRIVEARDDGPFLSKEDLNKKAGLSQKIIEYLDELGSLPNLPDKAQLSIFDM
                     "
     gene            7981..8448
                     /gene="rimP"
                     /locus_tag="O0293_001389"
     CDS             7981..8448
                     /gene="rimP"
                     /locus_tag="O0293_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCY0280278.1"
                     /translation="MSKITEQVEVIVKPIMEDLNFELVDVEYVKEGRDHFLRISIDKE
                     GGVDLNDCTLASEKISEAMDANDPIPEMYYLDVASPGAERPIKKEQDFQNAITKPVFV
                     SLYVPIEGEKEWLGILQEVNNETIVVQVKIKARTKDIEIPRDKIAKARHAVMI"
     gene            8469..9644
                     /gene="nusA"
                     /locus_tag="O0293_001390"
     CDS             8469..9644
                     /gene="nusA"
                     /locus_tag="O0293_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCY0280279.1"
                     /translation="MSSNELLLATEYLEKEKKIPRAVLIDAIEAALITAYKKNYDSAR
                     NVRVELNMDQGTFKVIARKDVVEEVFDDRDEVDLSTALVKNPAYEIGDIYEEDVTPKD
                     FGRVGAQAAKQAVMQRLRDAEREILFEEFIDKEEDILTGIIDRVDHRYVYVNLGRIEA
                     VLSEAERSPNEKYIPNERIKVYVNKVEQTTKGPQIYVSRSHPGLLKRLFEQEVPEIYD
                     GTVIVKSVAREAGDRSKISVFSENNDIDAVGACVGAKGARVEAVVEELGGEKIDIVQW
                     NEDPKVFVKNALSPSQVLEVIVDETNQSTVVVVPDYQLSLAIGKRGQNARLAAKLTGW
                     KIDIKSETDAREAGIYPVVEAEKVTEEDVALEDADTTESTEEVNDVSVETNVEKESE"
     gene            9665..9949
                     /locus_tag="O0293_001391"
     CDS             9665..9949
                     /locus_tag="O0293_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HCY0280280.1"
                     /translation="MKKKKIPMRKCILSNEMHPKKDMIRVVVNKEGEIFADVTGKKQG
                     RGAYVSKDVAMVEKAQQKEILEKYFKASKEQLDPVYKEIIRLIYREEIPK"
     gene            9946..10263
                     /locus_tag="O0293_001392"
     CDS             9946..10263
                     /locus_tag="O0293_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ family RNA-binding protein"
                     /protein_id="HCY0280281.1"
                     /translation="MSIDQILNFLGLAMRAGKVKTGESVIVNEIKKGNLKLVIVANDA
                     SDNTAKLITDKCKSYKVPFRKFGNRNELGIALGKGERVNVGITDPGFAKKLLSMIDEY
                     HKE"
     gene            10268..12385
                     /gene="infB"
                     /locus_tag="O0293_001393"
     CDS             10268..12385
                     /gene="infB"
                     /locus_tag="O0293_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCY0280282.1"
                     /translation="MSKQRIYEYAKELNLKSKEIIDELKSMNIEVSNHMQALEDDQIK
                     ALDKKFKKEQKNDNKQSTQNNHQKSNNQNQNKGQQKDNKKNQQQNNKGNKGNKKNNRN
                     NKKNNKNNKPQNQPAAPKEIPSKVTYQEGITVGEFADKLNVESSEIIKKLFLLGIVAN
                     INQSLNQETIELIADDYGVEVEEEVVINEEDLSIYFEDEKDDPEAIERPAVVTIMGHV
                     DHGKTTLLDSIRHTKVTAGEAGGITQHIGAYQIENDGKKITFLDTPGHAAFTTMRARG
                     AQVTDITILVVAADDGVMPQTIEAINHAKEAEVPIIVAVNKIDKPTSNPDRVMQELTE
                     YGLIPEDWGGETIFVPLSALSGDGIDDLLEMIGLVAEVQELKANPKNRAVGTVIEAEL
                     DKSRGPSASLLVQNGTLNVGDAIVVGNTYGRIRAMVNDLGQRIKTAGPSTPVEITGIN
                     DVPQAGDRFVVFSDEKQARRIGESRHEASIIQQRQESKNVSLDNLFEQMKQGEMKDLN
                     VIIKGDVQGSVEALAASLMKIDVEGVNVRIIHTAVGAINESDVTLANASNGIIIGFNV
                     RPDSGAKRAAEAENVDMRLHRVIYNVIEEIESAMKGLLDPEFEEQVIGQAEVRQTFKV
                     SKVGTIAGCYVTEGKITRNAGVRIIRDGIVQYEGELDTLKRFKDDAKEVAKGYECGIT
                     IENYNDLKEGDVIEAFEMVEIKR"
     gene            12771..13121
                     /gene="rbfA"
                     /locus_tag="O0293_001394"
     CDS             12771..13121
                     /gene="rbfA"
                     /locus_tag="O0293_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCY0280283.1"
                     /translation="MSSMRAERVGEQMKKELMDIINNKVKDPRVGFITITDVVLTNDL
                     SQAKVFLTVLGNDKEVENTFKALDKAKGFIKSELGSRMRLRIMPELMYEYDQSIEYGN
                     KIERMIQDLHKQDR"
BASE COUNT         4779 a         1795 c         2629 g         3945 t
ORIGIN      
        1 acgttatatt ttgtgatttg tcagtatcat attataatga cttatgttac gtatacagca
       61 atcattttta aaataaaaga aatttataaa caatcgaggt gtagcgagtg agctatttag
      121 ttacaataat tgcatttatt attgtttttg gtgtactagt aactgttcat gaatatggcc
      181 atatgttttt tgcgaaaaga gcaggcatta tgtgtccaga atttgcgatc ggtatggggc
      241 caaaaatttt tagttttaga aaaaatgaaa cactttacac tattaggtta ttgcctgttg
      301 gtggatatgt tcgtatggca ggagatggct tagaagagcc accagtcgag cccggtatga
      361 acgttaaaat taaacttaat gaagaaaatg aaataacaca tatcatatta gatgatcatc
      421 ataagtttca acaaattgaa gcgatcgaag ttaaaaaatg tgattttaag gatgacttat
      481 tcatagaagg tatcactgct tatgataatg aaagacatca ttttaaaatt gctagaaagt
      541 ctttctttgt tgaaaatggt agcttagttc aaattgctcc gagagacaga caatttgcac
      601 ataaaaagcc atggccgaaa tttttaacat tatttgcggg accgttattt aactttatat
      661 tagctttagt cctatttatt ggtcttgcat attatcaagg cacgcctacg tctactgtag
      721 aacaagtcgc agataagtat ccagctcaac aagcaggatt acaaaaaggt gataagatcg
      781 tccaaattgg caaatataaa atatctgaat ttgatgatgt tgataaggcg ttagataaag
      841 ttaaagataa taagacgact gttaaatttg aacgtgatgg taaaacaaag tcagttgaat
      901 taacacctaa aaagactgaa aaaaaactga ctaaagtaag ttcagagacg aagtatgttc
      961 tcggattcca accagcgagt gaacatacac tttttaaacc aattgtattc ggatttaaaa
     1021 gctttttaat cggtagtact tatattttta cagctgtagt aggtatgttg gctagtatat
     1081 ttacgggcgg attctcattt gatatgttaa atggtccggt tggtatttat cataacgtcg
     1141 actcagttgt taaagcgggt atcattagct taattggtta cactgcgtta ttaagtgtaa
     1201 acttaggtat tatgaattta attcctattc ctgcactaga cggtggtcgt attttatttg
     1261 ttatatatga agcgattttc agaaaaccag ttaataaaaa agcggaaaca acgattattg
     1321 ctattggtgc cattttcatg gtcgttataa tgatattagt aacgtggaat gatattcgac
     1381 gatatttctt ataatttagg aggataaata attatgaagc aatccaaagt ttttatacca
     1441 acgatgcgtg acgtgccatc agaagcagaa gcacaaagtc atcgtttatt attgaaatcg
     1501 ggtttgataa aacaaagtac aagtgggatt tatagttatt taccgctagc aacacgtgtg
     1561 ttaaataata ttactgcaat tgtgcgacaa gaaatggaac gtatcgattc tgttgaaatt
     1621 ttaatgccag cgttacaaca agctgaatta tgggaagaat caggacgttg gggtgcatat
     1681 ggcccagaat taatgcgttt acaagataga catggaagac aatttgcatt aggtccaaca
     1741 catgaagaat tagttacatc aatagtaaga aatgaattga aatcatacaa acaattaccg
     1801 atgacattat tccaaattca atctaaattc cgtgatgaaa agagaccacg ttttggttta
     1861 cttcgtgggc gtgaatttat tatgaaagat gcatattcat tccatgctga cgaggcatca
     1921 ttagatcaaa cgtatcaaga tatgtatcaa gcgtatagcc gtatttttga gagagttggc
     1981 attaacgcaa gaccagtagt tgcagattca ggtgctatag gcggtagcca tacacatgaa
     2041 tttatggcat taagtgctat cggtgaggat acaatcgttt acagtaaaga aagtgattat
     2101 gctgctaaca tcgaaaaagc agaagtcgtt tacgaaccaa atcataagca tactactgtg
     2161 caacctttag aaaaaattga aacaccaaat gttaagactg cgcaagaatt ggcagacttc
     2221 ttaggtagac cagtagatga aatcgttaaa acgatgattt tcaaagttga tggcgaatat
     2281 attatggttt tagtgcgtgg ccatcatgaa attaatgaca ttaaattaaa atcttatttc
     2341 ggcacagata atattgaatt agcaacacaa gacgaaattg ttaatttagt tggtgcaaat
     2401 cctggttcac taggtcctgt aattgataaa gaaatcaaaa tttatgcaga taattttgtg
     2461 caagatttaa ataatttagt tgtcggtgct aacgaagatg gttatcactt aattaatgta
     2521 aatgtaggta gagacttcaa cgttgatgaa tatggcgatt tccgttttat tttagaaggc
     2581 gaaaagttaa gtgatggttc aggcgttgca cattttgctg aaggtattga agttggtcaa
     2641 gtattcaaat tgggtactaa gtattcagaa tcaatgaatg ctacattctt agataaccaa
     2701 ggaaaagctc aatctttaat tatgggttgt tacggaattg gaatttctag aacgctaagt
     2761 gcgattgttg aacaaaatca cgatgataat ggaattgttt ggcctaaatc agttactccg
     2821 tttgatttac atttaatttc tattaatcct aagaaagatg atcaacgaga actagcagat
     2881 gcactatatg ctgaatttaa tactaaattt gatgtgttgt acgatgatcg tcaggaacgt
     2941 gcaggtgtta aatttaatga tgccgattta attggtttac cactgcgaat tgttgttggt
     3001 aaacgtgcat cggaaggtat tgtagaagtt aaagaacgtt taacaggtga tagcgaagaa
     3061 gttcacattg atgacttaat gactgtcatt acaaataaat atgataactt aaaataatta
     3121 agatcgaatg aattataaga gtaggaaaaa gctgaaagaa atctgatgct tatgtcctgc
     3181 tcttattatt tttgatataa tgattattcg atgaaaaatg actgaagaca tagtataatt
     3241 aaagataaat ttgttttaac aatataatga ttagccaaat ataaagcatt taattttcta
     3301 tcattactat gctcacataa tctaaatatt gttcgaacac gtaaaagtaa tttctattta
     3361 aggtggtaat tgtcttggca atgacagagc aacaaaaatt taaagtgctt gctgatcaaa
     3421 ttaaaatttc aaatcaatta gatgctgaaa ttttaaattc aggtgaactg acacgtatag
     3481 atgtttctaa caaaaacaga acatgggaat ttcatattac attaccacaa ttcttagctc
     3541 atgaagatta tttattattt ataaatgcaa tagagcaaga gtttaaagat atcgccaacg
     3601 ttacatgtcg ttttacggta acaaatggca cgaatcaaga tgaacatgca attaaatact
     3661 ttgggcactg tattgaccaa acagctttat ctccaaaagt taaaggtcaa ttgaaacaga
     3721 aaaagcttat tatgtctgga aaagtattaa aagtaatggt atcaaatgac attgaacgta
     3781 atcattttga taaggcatgt aatggaagtc ttatcaaagc gtttagaaat tgtggttttg
     3841 atatcgataa aatcatattc gaaacaaatg ataatgatca agaacaaaac ttagcttctt
     3901 tagaagcaca tattcaagaa gaagacgaac aaagtgcacg attggcaaca gagaaacttg
     3961 aaaaaatgaa agctgaaaaa gcgaaacaac aagataacaa cgaaagtgct gtcgataagt
     4021 gtcaaattgg taagccgatt caaattgaaa atattaaacc aattgaatct attattgagg
     4081 aagagtttaa agttgcaata gagggtgtca tttttgatat aaacttaaaa gaacttaaaa
     4141 gtggtcgcca tatcgtagaa attaaagtga ctgactatac ggactcttta gttttaaaaa
     4201 tgtttactcg taaaaacaaa gatgatttag aacattttaa agcgctaagt gttggtaaat
     4261 gggttagggc tcaaggtcgt attgaagaag atacatttat tagagattta gttatgatga
     4321 tgtctgatat tgaagagatt aaaaaagcga caaaaaaaga taaggctgaa gaaaagcgtg
     4381 tagaattcca cttgcatact gcaatgagcc aaatggatgg tatacccaat attggtgcgt
     4441 atgttaaaca ggcagcagac tggggacatc cagccattgc ggttacagac cataatgttg
     4501 tgcaagcatt tccagatgct cacgcagcag cggaaaaaca tggcattaaa atgatatacg
     4561 gtatggaagg tatgttagtt gatgatggtg ttccgattgc atacaaacca caagatgtcg
     4621 tattaaaaga tgctacttat gttgtgttcg acgttgagac aactggttta tcaaatcagt
     4681 atgataaaat catcgagctt gcagctgtga aagttcataa cggtgaaatc atcgataagt
     4741 ttgaaaggtt tagtaatccg catgaacgat tatcggaaac gattatcaat ttgacgcata
     4801 ttactgatga tatgttagta gatgcccctg agattgaaga agtacttaca gagtttaaag
     4861 aatgggttgg cgatgcgata ttcgtagcgc ataatgcttc gtttgatatg ggcttcatcg
     4921 atacgggata tgaacgtctt gggtttggac catcaacgaa tggtgttatc gatactttag
     4981 aattatctcg tacgattaat actgaatatg gtaaacatgg tttgaatttc ttggctaaaa
     5041 aatatggcgt agaattaacg caacatcacc gtgccattta tgatacagaa gcaacagctt
     5101 acattttcat aaaaatggtt caacaaatga aagaattagg cgtattaaat cataacgaaa
     5161 tcaacaaaaa actcagtaat gaagatgcat ataaacgtgc aagacctagt catgtcacat
     5221 taattgtaca aaaccaacaa ggtcttaaaa atctatttaa aattgtaagt gcatcattgg
     5281 tgaagtattt ctaccgtaca cctcgaattc cacgttcatt gttagatgaa tatcgtgagg
     5341 gattattggt aggtacagcg tgtgatgaag gtgaattatt tacggcagtt atgcagaagg
     5401 accagagtca agttgaaaaa attgccaaat attatgattt tattgaaatt caaccaccgg
     5461 cactttatca agatttaatt gatagagagc ttattagaga tactgaaaca ttacatgaaa
     5521 tttatcaacg tttaatacat gcaggtgaca cagcgggtat acctgttatt gcgacaggaa
     5581 atgcacacta tttgtttgaa catgatggta tcgcacgtaa aattttaata gcatcacaac
     5641 ccggcaatcc acttaatcgc tcaactttac cggaagcaca ttttagaact acagatgaaa
     5701 tgttaaacga gtttcatttt ttaggtgaag aaaaagcgca tgaaattgtt gtgaaaaata
     5761 caaacgaatt agcagatcga attgaacgtg ttgttcctat taaagatgaa ttatacacac
     5821 cgcgtatgga aggtgctaac gaagaaatta gagaactaag ttatgcaaat gcgcgtaaac
     5881 tgtatggtga agacctgcct caaatcgtaa ttgatcgatt agaaaaagaa ttaaaaagta
     5941 ttatcggtaa tggatttgcg gtaatttact taatttcgca acgtttagtt aaaaaatcat
     6001 tagatgatgg atacttagtt ggttcccgtg gttcagtagg ttctagtttt gtagcgacaa
     6061 tgactgagat tactgaagta aacccgttac cgccacacta tatttgtccg aactgtaaaa
     6121 cgagtgaatt tttcaatgat ggttcagtag gatcaggatt tgatttacct gataagacgt
     6181 gtgaaacttg tggagcgcca cttattaaag aaggacaaga tattccgttt gaaacatttt
     6241 taggatttaa gggagataaa gttcctgata tcgacttaaa ctttagtggt gaatatcaac
     6301 cgaatgccca taactacaca aaagtattat ttggtgagga taaagtattc cgtgcaggta
     6361 caattggtac tgttgctgaa aagactgctt ttggttatgt taaaggttat ttgaatgatc
     6421 aaggtatcca caaaagaggt gctgaaatag atcgactcgt taaaggatgt acaggtgtta
     6481 aacgtacaac tggacagcat ccagggggta ttattgtagt acctgattac atggatattt
     6541 atgattttac gccgatacaa tatcctgccg atgatcaaaa ttcagcatgg atgacgacac
     6601 attttgattt ccattctatt catgataatg tattaaaact tgatatactt ggacacgatg
     6661 atccaacaat gattcgtatg cttcaagatt tatcaggaat tgatccaaaa acaatacctg
     6721 tagatgataa agaagttatg cagatattta gtacacctga aagtttgggt gttactgaag
     6781 atgaaatttt atgtaaaaca ggtacatttg gggtaccaga attcggtaca ggattcgtgc
     6841 gtcaaatgtt agaagataca aagccaacaa cattttctga attagttcaa atctcaggat
     6901 tatctcatgg tacagatgtg tggttaggca atgctcaaga attaattaaa accggtatat
     6961 gtgatttatc aagtgtaatt ggttgtcgtg atgatatcat ggtttattta atgtatgctg
     7021 gtttagaacc atcaatggct tttaaaataa tggagtcagt acgtaaaggt aaaggtttaa
     7081 ctgaagaaat gattgaaacg atgaaagaaa atgaagtgcc agattggtat ttagattcat
     7141 gtcttaaaat taagtacatg ttccctaaag cccatgcagc agcatacgtt ttaatggcag
     7201 tacgtatcgc atatttcaaa gtacatcatc cactttatta ctatgcatct tactttacaa
     7261 ttcgtgcgtc agactttgat ttaatcacga tgattaaaga taaaacaagc attcgaaata
     7321 ctgtaaaaga catgtattct cgctatatgg atctaggtaa aaaagaaaaa gacgtattaa
     7381 cagtcttgga aattatgaat gaaatggcgc atcgaggtta tcgaatgcaa ccgattagtt
     7441 tagaaaagag tcaggcgttc gaatttatca ttgaaggcga tacacttatt ccgccgttca
     7501 tatcagtgcc tgggcttggc gaaaacgttg cgaaacgaat tgttgaagct cgtgacgatg
     7561 gcccattttt atcaaaagaa gatttaaaca aaaaagctgg attatctcag aaaattattg
     7621 agtatttaga tgagttaggc tcattaccga atttaccaga taaagctcaa ctttcgatat
     7681 ttgatatgta aaatgaaata atcaaggtat ttatttaatg cgtatggcgt agtcaaagaa
     7741 atacaaaatt gttgctggac acaaaattat gcccgtattt cttttcaatg tcttacgagt
     7801 ctattcaaat gtaatggtga aataaaggaa caaactttta caagaatctc tgattaatag
     7861 tgaagtcatt tgtttcaagc ataaacttat gctataatta agttgcttaa aaattagtga
     7921 actcaggcag aagagtggga gattcccgct cttttctatt tgccaaaaag ggaggcctgt
     7981 atgagtaaaa ttacagaaca agtagaagtg attgttaaac caattatgga agacttgaat
     8041 tttgaacttg tagacgttga atatgtcaaa gagggtagag atcattttct tagaatctct
     8101 attgataaag aaggtggcgt agatttaaat gattgtacgc tagcttctga aaaaataagt
     8161 gaagctatgg atgcaaatga tcctattcct gaaatgtatt atttagacgt agcgtcacct
     8221 ggtgcagaac gtccaattaa aaaagaacaa gatttccaaa atgcaataac taaacctgta
     8281 tttgtttctt tatatgtacc aattgaaggt gaaaaggaat ggttaggcat tttacaagaa
     8341 gtcaataatg aaacaattgt agtacaagtt aaaatcaaag caagaacgaa agatatagag
     8401 ataccgagag acaaaatagc aaaagcacgt cacgcagtta tgatttaacg tgatgaggag
     8461 gaaaaaacgt gtcaagtaat gaattattat tagctactga gtatttagaa aaagaaaaga
     8521 agattcctag agcagtatta attgatgcta ttgaagcagc tttaattact gcatacaaaa
     8581 agaattatga tagtgcaaga aatgtccgtg tggaattaaa tatggatcaa ggtactttca
     8641 aagttatcgc tcgtaaagat gttgttgaag aagtatttga cgacagagat gaagtggatt
     8701 taagtacagc gcttgttaaa aaccctgcat atgaaattgg tgatatatac gaagaagatg
     8761 taacacctaa agattttggt cgtgtaggtg ctcaagcagc gaaacaagca gtaatgcaac
     8821 gtcttcgtga tgctgaacgt gaaattttat ttgaagaatt tatagacaaa gaagaagaca
     8881 tacttactgg aattattgac cgtgttgacc atcgttatgt atatgtgaat ttaggtcgta
     8941 tcgaagctgt tttatctgaa gcagaaagaa gtcctaacga aaaatatatt cctaacgaac
     9001 gtatcaaagt atatgttaac aaagtggaac aaacgacaaa aggtcctcaa atctatgttt
     9061 ctcgtagcca tccaggttta ttaaaacgtt tatttgaaca agaagttcca gaaatttacg
     9121 atggtactgt aattgttaaa tcagtagcac gtgaagctgg cgatcgctct aaaattagtg
     9181 tcttctctga aaacaatgat atagatgctg ttggtgcatg tgttggtgct aaaggcgcac
     9241 gtgttgaagc tgttgttgaa gagctaggtg gtgaaaaaat cgacatcgtt caatggaatg
     9301 aagatccaaa agtatttgta aaaaatgctt taagcccttc tcaagtttta gaagttattg
     9361 ttgatgaaac aaatcaatct acagtagttg ttgttcctga ttatcaattg tcattagcga
     9421 ttggtaaaag aggacaaaac gcacgtctag ctgctaaatt aaccggctgg aaaattgata
     9481 ttaaatcaga aacagatgcg cgtgaagcgg gtatctatcc agtagttgaa gctgaaaaag
     9541 taactgaaga agatgttgct ttagaagatg ctgacacaac agaatcaacc gaagaggtaa
     9601 atgatgtttc agttgaaaca aatgtagaga aagaatctga ataataggtt ggagtgaagt
     9661 atctatgaaa aagaaaaaaa ttccgatgcg aaaatgtatt ctttcaaatg aaatgcatcc
     9721 caaaaaagat atgattcgtg ttgttgttaa taaagaaggc gaaatctttg cggatgttac
     9781 tggaaagaaa caaggccgtg gcgcatatgt ttctaaagat gttgctatgg ttgaaaaagc
     9841 acaacaaaaa gaaattttag aaaaatattt taaagcatct aaagagcaat tggatcctgt
     9901 ttacaaagaa attattagat taatttatag agaagagatc ccaaaatgag tatagatcaa
     9961 atattaaact ttttaggatt agcaatgaga gctggtaaag taaaaacagg tgaatcagtc
    10021 attgttaatg agattaaaaa aggaaatttg aagctcgtta ttgttgcaaa tgatgcgtct
    10081 gataatacag ctaaattaat tacagataaa tgtaagagtt acaaagttcc attcagaaag
    10141 tttggaaatc gaaatgaatt gggaatagca cttggaaaag gtgagcgtgt taatgtaggg
    10201 attactgacc caggctttgc taaaaagttg ctatcaatga tagatgaata tcataaggag
    10261 tgattatatg agtaaacaaa gaatttacga atatgcgaaa gaattaaatc taaagagtaa
    10321 agagattata gatgagttaa aaagcatgaa tattgaggtt tcaaatcata tgcaagcttt
    10381 ggaagatgac caaattaaag cattagataa aaagttcaaa aaagaacaaa agaacgacaa
    10441 taaacaaagc actcaaaata atcaccaaaa atcaaacaat caaaaccaaa ataaagggca
    10501 acaaaaagat aacaaaaaga atcaacaaca aaataataaa ggcaacaaag gcaataaaaa
    10561 gaataataga aataataaga aaaataacaa gaataataaa ccacaaaatc aaccagctgc
    10621 tccaaaagaa ataccatcaa aagtgacata tcaagaaggt attacagtag gcgaatttgc
    10681 ggataaatta aatgttgaat catcagaaat tatcaaaaaa ttattcttac ttggtattgt
    10741 tgctaatatc aatcaatcat taaatcaaga aacaatcgaa ttaattgccg atgattatgg
    10801 cgttgaggtt gaagaagaag ttgtgattaa tgaagaagac ttatcaatct atttcgaaga
    10861 cgaaaaagat gatccagagg caattgagag accagcagtt gtaacaatta tgggacatgt
    10921 tgaccatggt aaaacgactt tattagattc aattcgtcat acaaaagtta cagcaggtga
    10981 agcaggcgga atcactcaac atattggtgc atatcaaatt gaaaacgatg gcaaaaaaat
    11041 cactttctta gatacaccgg gacatgctgc atttacaacg atgcgtgcgc gtggtgcaca
    11101 agtaacagat attactattt tagtagtagc agctgacgat ggtgttatgc cacaaacaat
    11161 tgaagcaatt aaccatgcta aagaagcaga agtaccaatt attgttgcag taaataaaat
    11221 tgataaacca acttcaaatc ctgatcgagt tatgcaagaa ttaactgaat atggtttaat
    11281 tcctgaagat tggggcggcg aaacaatttt cgttccactt tctgcattaa gtggtgatgg
    11341 tatcgacgat ttattagaaa tgataggatt agttgcagaa gttcaagaac ttaaagcaaa
    11401 tcctaaaaac cgtgctgttg gtacagttat cgaagctgaa ttagataaat cacgtggtcc
    11461 ttctgcatca ttattagtac aaaacggtac attaaatgtt ggtgatgcga ttgtagttgg
    11521 taatacttac ggccgtatcc gtgcaatggt taatgactta ggtcaaagaa tcaaaacggc
    11581 tggtccatca acgcctgttg aaattacagg tattaatgat gtgccacaag ctggggatcg
    11641 ctttgttgta tttagtgatg aaaaacaagc tcgtcgtatt ggtgaatcaa gacacgaagc
    11701 tagcattata caacaacgtc aagaaagtaa aaatgtttca ttagataacc tgtttgaaca
    11761 aatgaaacaa ggtgaaatga aagatttaaa cgttattatt aaaggtgatg ttcaaggttc
    11821 tgttgaagct ttagctgcat cattaatgaa aattgatgtt gaaggcgtaa atgttcgtat
    11881 cattcataca gcggttggtg caattaatga gtcagacgtg acacttgcta atgcctcaaa
    11941 tggtattatc attggtttca atgttcgtcc agacagtggt gcaaaacgtg ctgcagaagc
    12001 tgaaaatgtt gatatgcgtt tacacagagt tatttataat gttatcgaag aaattgaatc
    12061 agcgatgaaa ggtttacttg atccagaatt tgaagaacaa gttatcggac aagctgaagt
    12121 tcgtcaaaca ttcaaagttt ctaaagttgg tactattgct ggatgttatg ttactgaagg
    12181 taaaattacg cgaaatgctg gtgtacgtat tattcgtgat ggtattgttc aatatgaagg
    12241 cgaattagat acacttaaac gtttcaaaga tgatgctaag gaagttgcaa aaggttatga
    12301 atgtggtatt acaattgaaa actacaatga ccttaaagaa ggcgatgtta tcgaagcatt
    12361 tgaaatggtt gaaattaagc gttaattaaa taaattacaa gctaaaagta tagttaagat
    12421 tgatatgctc cctataaata ttgcactttt taagtgtcta ctttataggg agcatatttg
    12481 atactagctt ttggtttttt attagaatag attacctatt aaaagttacg ttatatggac
    12541 atgattttgt ataaaatttt gtggtggcct agaatgattt ttaatgacaa aatataatgt
    12601 cgactattat tggaaaattt tctgttgaaa tgcctatctt acggcaaact ttatttgatt
    12661 ttataggctt aatttattaa aataacgtgt gagctaaaat aattgtttaa gcattgttac
    12721 actaaaaaat gcaaataaca attgaactta aagataaaga ggtgacaaga atgagcagta
    12781 tgagagcaga gcgtgttggt gaacaaatga agaaggaatt aatggatatc atcaacaata
    12841 aagtcaaaga tcctcgagtt ggttttatta caattacaga tgttgtttta acaaatgatt
    12901 tatcgcaggc taaagtattt ttaactgtat taggtaacga taaagaagta gaaaatacat
    12961 ttaaagcact tgataaagca aaaggcttca ttaagtctga attaggttct agaatgcgat
    13021 tacgtattat gccggaatta atgtatgaat atgatcaatc aatcgaatat ggtaataaaa
    13081 ttgaacgaat gattcaagat ttacacaaac aagatagata atttagtgtt aggtatctgg
    13141 aaaatgtt
//
LOCUS       DAKTIO010000065        12990 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.065, whole genome shotgun sequence.
ACCESSION   DAKTIO010000065 DAKTIO010000000
VERSION     DAKTIO010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12990
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.065"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(51..326)
                     /locus_tag="O0293_001395"
     CDS             complement(51..326)
                     /locus_tag="O0293_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCY0280284.1"
                     /translation="MENKFVPGILIGAVIGGAISLADKSTRQALVQSVKDAKNGNRTR
                     KPSKVSKIKDEVLYWKDVVEEIRRNNPELERSLKDAKETFVNRKNQR"
     gene            complement(333..797)
                     /locus_tag="O0293_001396"
     CDS             complement(333..797)
                     /locus_tag="O0293_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HCY0280285.1"
                     /translation="MVDVAFVCLGNICRSPMAEAIMRQRLKDRNIHDIKVHSRGTGSW
                     NLGEPPHEGTQKILNKHNIPFDGMISELFEATDDFDYIVAMDQSNVDNIKSINPNLKG
                     QLFKLLEFSNMEESDVPDPYYTNNFEGVYDMVLSSCDNLIDYIVKDANLKEG"
     gene            944..1144
                     /locus_tag="O0293_001397"
     CDS             944..1144
                     /locus_tag="O0293_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1128 family protein"
                     /protein_id="HCY0280286.1"
                     /translation="MTNEEKVLAIREKLNIVNQGLLDPEKYKNANEEELTDIYDFVQS
                     RERLSPSEVTAIADALGQLRHE"
     gene            1147..2403
                     /locus_tag="O0293_001398"
     CDS             1147..2403
                     /locus_tag="O0293_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCY0280287.1"
                     /translation="MGILTNYKEKLQQYAELLVKVGMNVQPKQPVFIRSSVETLELTH
                     LIVEEAYHCGASDVRVVYSDPTLKRLKFENESVEHFANHEIKSYDVEARMDYVKRGAA
                     NLALISEDPDLMDGIDSQKLQAFQQQNARAFKGYMESVQKNQFPWVVAAFPSKAWAKR
                     VYPELSVEEAYIKFIDEVFDIVRIDGNDPVENWRQHIANLSVYAQKLQQKNYHALHYV
                     SEGTDLTVGLAKNHIWEDATSYVNGKEQAFIANIPTEEVFTAPDRNRVDGYVTNKLPL
                     SYNGTIIDQFKLMFKDGEIIDFSAGKGEAVLKDLINTDEGSRRLGEVALVPDDSPISN
                     RNTIFYNTLFDENAACHLAIGSAYAFNIQGGTEMTVEEKIASGLNDSNVHVDFMIGSS
                     DLTIYGIFEDGSKELVFENGNWASTF"
     gene            2493..3023
                     /locus_tag="O0293_001399"
     CDS             2493..3023
                     /locus_tag="O0293_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCY0280288.1"
                     /translation="MTNQDRPMKSMSESKCYKNRQVFPQDTNHHHTMFGGTLMANIDE
                     IAAITAMKHAGAQVVTASTDSVDFLKPIKTGDILQYVAMVSYAGTSSMEVVVQIRIDD
                     VFNNKHDLAALSYLTFVALDDEGKPKHVPGVYPEDDVEKWFYDTAPQRVERRKARRIE
                     SKQTIEYLAQVQHIRD"
     gene            complement(3283..4434)
                     /gene="yfkAB"
                     /locus_tag="O0293_001400"
     CDS             complement(3283..4434)
                     /gene="yfkAB"
                     /locus_tag="O0293_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/CxCxxxxC motif protein YfkAB"
                     /protein_id="HCY0280289.1"
                     /translation="MNSVKLKQPVSIYNDPWEAYNDVKEHGQLTLSNIEFTTTNLCNM
                     RCSHCAVGYTLQTVDPEPLDMDLIYRRLDEIPNLRTMSITGGEPMFSKKSIRNVVKPL
                     LKYAHHRGIYTQMNSNLTLPQDRYLDIAEYIDVMHISHNWGTTDEFANVGFGAMKKQP
                     PLKAKLKLYEQMISNARTLSEQGMFVSAETMLNQSTLPHLRKIHQEVVHDMKCSRHEI
                     HPMYPADFASQLNVLTLAEMKKTIHDILDFRDEDIWMLFGTLPVFPCLKDDEDQKLLS
                     RLRNAKNVTTRNDPDGRSRLNVNVFTGNVIVTDFGDETGTISNIQKDKLTDVFDKWLS
                     SDLAKSLNCHCSEFSCLGPNVLVKNMYYPNMDFKDNERHMHKQPQIIQF"
     gene            4658..4819
                     /locus_tag="O0293_001401"
     CDS             4658..4819
                     /locus_tag="O0293_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280290.1"
                     /translation="MNEQQTIEQIKARLNKFIEDIDHVNPDEVRVEDIDEWIGLLDQL
                     EEKVKLVSK"
     gene            4950..5465
                     /locus_tag="O0293_001402"
     CDS             4950..5465
                     /locus_tag="O0293_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCY0280291.1"
                     /translation="MTKKVAIILANEFEDIEYSSPKEALENAGFNTVVIGDTANSEVV
                     GKHGEKVTVDVGIAEAKPEDYDALLIPGGFSPDHLRGDTEGRYGTFAKYFTKNDVPTF
                     AICHGPQILIDTDDLKGRTLTAVLNVRKDLSNAGAHVVDESVVVDNNIVTSRVPDDLD
                     DFNREIVKQLQ"
     gene            5806..6615
                     /gene="sgtB"
                     /locus_tag="O0293_001403"
     CDS             5806..6615
                     /gene="sgtB"
                     /locus_tag="O0293_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional peptidoglycan glycosyltransferase
                     SgtB"
                     /protein_id="HCY0280292.1"
                     /translation="MKRSDRYSNSNEHFEHMKHEPHYNTYYQPVGKPPKKKKSKRILL
                     KILLTILIIIALFIGIMYFLSTRDNVDELRKIENKSSFVSADNMPEYVKGAFISMEDE
                     RFYNHHGFDLKGTTRALFSTISDRDVQGGSTITQQVVKNYFYDNDRSFTRKVKELFVA
                     HRVEKQYNKNEILSFYLNNIYFGDNQYTLEGAANHYFGTTVNKNSTTMSHITVLQSAI
                     LASKVNAPSVYNINNMSENFTQRVSTNLEKMKQQNYINETQYQQAMSQLNR"
     gene            6876..7694
                     /gene="recX"
                     /locus_tag="O0293_001404"
     CDS             6876..7694
                     /gene="recX"
                     /locus_tag="O0293_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCY0280293.1"
                     /translation="MPKITKIEVQKKNKERFNLFLDEQFEMGIDIDTLVKFNLKKGQQ
                     LEAADMAEIQKYDHYRIGLNKAIQYLSYKKRTEKEVIQYLQKEEISEQAISEVIEYCY
                     REKLIDHQDYAESLKNTMIRTTDKGPKIYQQKLYQLGIEPNIIEIFTELYREQQELDD
                     IIQIAEKISKTKKGPQNKVKEKVMQSLIQKGFEMETIHAVLNEMDFTQDEAVLDDLLQ
                     RDLEKIYNKNRKKYTQQKLISKTIEGLMRKGYKYDKIKAKLEESGIADGTEEIE"
     gene            7672..7986
                     /locus_tag="O0293_001405"
     CDS             7672..7986
                     /locus_tag="O0293_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhH family protein"
                     /protein_id="HCY0280294.1"
                     /translation="MEQKKLSEMSEPELRHEIQLYKEKMRKAEMNGILNEYDVYQSKV
                     IVAESYLVDRKKIEIGKIYKLTDGSNQYFKVERLKGIFAWGFRFNSDEPEEGLPIALL
                     QL"
     gene            8001..9518
                     /locus_tag="O0293_001406"
     CDS             8001..9518
                     /locus_tag="O0293_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0280295.1"
                     /translation="MGSSIVLKLLKVTHYYRNKQNKKWYLPFGYDAEDIDLNNISLHI
                     YQGEALGIIGEPESSKALVGQLLAGAIKPDKGKVVCTEDLFYGYIEDQSLIHQTVEAY
                     TAQLVQLFPYEINDHKAEQIIQYAHLGDYKTKPVNHISKAAYAQLLLSIARSSKSNII
                     ILNHVIDYLTPQFMERAIELTNDYIENNLTIVSIGDDIDKISQVSNYIAWFSHGQLRM
                     EGSLKQVIPSFKEHERDRLSLNSKEEIENFDLDWKKNRTRIPEMTYNFKRVERYNHAK
                     PPKFLVRFWTLASGTILGLALMMLLIFNNIGIISITDFTNRATMQNENKDPYGEKLAY
                     GIAFNGSVDMQGDKQVTIPKYSVVTITGENSKNYRVTADNKTYYVSKDKLEYFNPAGL
                     YQTHSFKKLAPYMKSNYSNYYAYFNSQLHKKHSSVIKTLVPDDDNRFVASVTQQPIQL
                     LFNDNNQLYGFVYPIVDKKELKDKFNINNNIWITKVGNGYCIANLKEDKWIYIEL"
     gene            9527..10363
                     /locus_tag="O0293_001407"
     CDS             9527..10363
                     /locus_tag="O0293_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280296.1"
                     /translation="MLDNIILYFKNLPHTKRYVTERLKQSWKSFLIVLAACLILIIAS
                     ETLFSFSHLTDVKEVRWLFRIIVLIVFAVVMFTIYISYHHYMNDFLVTKLFNISAATP
                     VVIMSILSFIMLVILTMISALVKPVNFETSYIALFYFIVMATIFVGLISVTFGLIRLL
                     TEKINIIFYGVCVLCFLLLPIIFIPNPNHVFINHILMLNPMYYIVNGIAQSIIFGISS
                     MENIPYHFYFILFLCLIAAVNFVLARYTTHAIYNKTSKVTQTDNQQDVSNDSTDEADT
                     SS"
     gene            complement(10624..11601)
                     /locus_tag="O0293_001408"
     CDS             complement(10624..11601)
                     /locus_tag="O0293_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCY0280297.1"
                     /translation="MDTATHIAIGVGLTALATQDPAMASTFGATATTLIVGSLIPDGD
                     TVLKLKDNATYISHHRGITHSIPFTILWPILITFLIFTFFSGTNPFHVWMWAQLAVFL
                     HVFVDIFNSYGTQALRPITNKWIQLSVINTFDPIIFTVLCIGIVLWVIGLHPFAVFFP
                     IIALLIIYYMIRFKMRAVIKQQALKAIQQEHHPVKVFVAPTIKFMEWRVAIQTDAHDY
                     VGKAYGRNVVFSDKVERQTLSTDSILWKVKGNKDIRTFLNFSSIYRWQTTTLADGSTE
                     IRLIDLRYLKNDHYSFVAIAHVTNDNVIDHSYIGWVFTEDKLQRKLYAK"
     gene            11753..12790
                     /gene="mutY"
                     /locus_tag="O0293_001409"
     CDS             11753..12790
                     /gene="mutY"
                     /locus_tag="O0293_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCY0280298.1"
                     /translation="MYQQSSFKENLIHWFDENQREMPWRQTTNPYYIWLSEVMLQQTQ
                     VKTVIDYYHRFVERFPTVEVLSQASEDEVLKYWEGLGYYSRARNFHTAIKEVHDKYEG
                     LVPKDPDQFKALKGVGPYTQAAVMSIAYNVPLATVDGNVFRVWSRLNDDYRDIKLQST
                     RKSYEQELLPYVTTEAGTFNQAMMELGALICTPKNPLCLFCPVQENCEAFDKGTFEKL
                     PVKSKNVSKKVIEQSVFLIRNNQGQYLLQKRSEKLLHGMWQFPMFESEHARRKMTEKI
                     GHDIQPVETPIFELKHQFTHLTWKIKVYAVSGAINIETLPDDMIWFDLSDRDQYTFPV
                     PMSKIYQFING"
BASE COUNT         4689 a         1826 c         2248 g         4227 t
ORIGIN      
        1 aaaggcgtac accaaattgt taagtattgc cttataaaaa caaaatcatc ttagcgttga
       61 ttttttctat taacaaatgt ttctttcgca tcctttaatg aacgttctaa ttcaggatta
      121 ttacgacgaa tttcttcaac aacatctttc cagtataaaa cttcgtcttt aatcttgctg
      181 actttagaag gcttacgagt gcggttacca ttttttgcat ctttaactga ttgaactaaa
      241 gcttgacgtg tagatttatc agctaaacta attgcaccac caattacggc accaattaaa
      301 ataccaggaa caaatttatt ttccataaaa aactacccct ctttcaaatt tgcatctttt
      361 acgatgtagt ctattaaatt atcacaagat gataatacca tgtcgtatac accttcaaaa
      421 ttattcgtgt agtatggatc tggtacatca ctctcttcca tattactaaa ttctaacagt
      481 ttgaacaatt gtcccttaag attaggattg atagatttaa tattatcaac gttactttga
      541 tccatagcca caatgtaatc aaaatcatct gtcgcttcga ataattcact aatcatgcca
      601 tcaaatggaa tattgtgttt gttgagaatt ttttgtgtac cttcatgagg tggctctcct
      661 aaattccagc taccagtacc tcttgaatgt actttaatat catgaatatt tctgtcttta
      721 agtctttgtc gcatgattgc ttctgccatt ggagaacgac atatattgcc aagacagaca
      781 aatgctacat ctaccatttt gattcctcca aactatgtag ttatatcccc attttatagc
      841 gactttaaac aataagaaag cagattatat aaaattctat taaagtttat taaattgtga
      901 tactttgata acataactat tattagaggt gaacattgtg gctatgacaa atgaagagaa
      961 agtattagct attagagaga agttaaatat tgttaatcaa ggattattag atcctgaaaa
     1021 atataaaaat gcaaatgaag aagaattaac agatatatat gattttgttc aatcaagaga
     1081 aagattgtcg ccaagtgaag tgacagctat tgctgacgct ttaggacaat tgcgacacga
     1141 ataggagtgg gaattttgac taattacaaa gaaaagttac aacaatacgc tgaactatta
     1201 gttaaggttg gtatgaatgt tcaaccaaaa cagccagtat ttattagatc atcagtagaa
     1261 acattagaat taacgcattt aattgtagaa gaagcatatc attgtggcgc ttcagatgta
     1321 cgtgttgtat atagcgatcc aaccttaaaa cgcttaaaat ttgaaaatga atctgtggaa
     1381 cattttgcta atcatgagat aaaatcatat gatgttgaag cgagaatgga ttacgttaaa
     1441 cgtggcgctg ctaatttagc acttattagt gaagatcctg atttaatgga tggtatcgat
     1501 agccaaaaat tacaagcatt tcaacaacaa aacgcacgcg catttaaagg atatatggaa
     1561 agtgtacaaa aaaatcaatt tccatgggtc gtagctgctt ttccatctaa agcttgggcg
     1621 aagagagtgt atcctgaact atctgtagaa gaagcatata ttaagtttat tgacgaggta
     1681 ttcgatattg tgcgaattga tggtaacgat ccagttgaaa attggcgtca acacatcgct
     1741 aatttaagtg tttacgctca aaagttacaa caaaaaaatt accatgcgtt gcattatgta
     1801 tctgaaggta ctgatttaac agtaggttta gcgaaaaatc atatctggga agatgctaca
     1861 agttatgtta acggcaagga acaagctttt attgctaata taccaactga agaagtattt
     1921 acagcaccag atcgaaatag agtagatgga tatgttacta ataaattgcc tttaagttat
     1981 aatggcacaa ttatcgatca atttaaattg atgtttaaag atggagagat tattgatttt
     2041 tcagctggaa aaggtgaagc ggtattgaaa gatttaatca atactgatga aggttcaaga
     2101 agattaggtg aagtagcatt agtacctgat gattcaccga tatcaaatcg taataccatt
     2161 ttttacaata ctttatttga tgaaaatgca gcatgtcatt tagcgattgg atctgcttac
     2221 gcctttaata ttcaaggtgg aacggaaatg actgttgagg aaaagattgc aagcggatta
     2281 aatgattcaa atgtacatgt cgatttcatg attggtagta gtgatttgac tatttatggc
     2341 atattcgaag atggttcaaa agaactagta tttgaaaatg gaaattgggc atcaacattt
     2401 taataaatgt tattttgagg tgctgagtag gaaatgaaac atgtatttaa aggtcaaata
     2461 cgtgattaaa gtatagattg ggagataaaa taatgacaaa tcaggacaga ccaatgaaat
     2521 ctatgtcaga atcaaaatgt tataaaaata gacaagtttt ccctcaagat acgaatcacc
     2581 atcatacaat gtttggtggt acattgatgg ctaatattga tgaaattgca gcaatcacag
     2641 ctatgaaaca tgctggtgcg caagtagtta ccgcatctac agactcagta gatttcttaa
     2701 agccgattaa aacaggggac atattacaat acgtagcgat ggtttcatac gctgggacta
     2761 gttcaatgga agtggtcgtt caaattagaa ttgacgatgt atttaataac aagcatgatt
     2821 tagctgcatt aagttactta acatttgttg cgttagatga tgaaggcaaa cctaaacatg
     2881 tacctggtgt atatccagaa gatgatgttg aaaaatggtt ttatgataca gcaccacaac
     2941 gtgttgaacg aagaaaagca cgtcgcattg aaagtaaaca aacaattgaa tatttagctc
     3001 aagtgcaaca tattagagac taaaaatata aagacagatt gttaaggatg aattgaactg
     3061 ttatgttcga atattccacc tacaatctgt ctttttgatt atgcacgttt atgcttttag
     3121 ttaaaattta ccccttttat tatagactat tagtcctatt aaatgccctt tattatttaa
     3181 gtagagtact gccagcaatt aattgttttt tattttataa tagcattaaa atatgcggag
     3241 actacttatc gataaagtgc ttctccgcat aattaagagt ttttaaaatt gtataatttg
     3301 tggttgtttg tgcatatgac gctcattatc tttaaaatcc atattcggat agtacatatt
     3361 tttaacaaga acatttggtc ctaaacaact aaactcggaa caatgacaat ttaatgattt
     3421 agcaagatca gaggataacc atttatcaaa tacatctgtt aatttatctt tttgtatatt
     3481 cgaaattgta cctgtttcat ctccgaaatc agttacgatt acattacctg taaatacatt
     3541 gacatttaaa cgactacggc catccgggtc atttctagtc gttacatttt tagcatttct
     3601 taaacgtgat agtaactttt gatcttcatc atcctttaag catggaaaca caggcaaagt
     3661 accaaataac atccaaatat cttcatctct gaaatccaat atatcatgaa ttgtcttttt
     3721 catttccgct agagttaaca catttaattg acttgcaaag tcagctggat acatagggtg
     3781 aatctcgtgt ctgctacatt tcatatcatg aacgacttct tgatgtattt ttcgtaaatg
     3841 tggtagcgta ctttgattga gcattgtttc cgcagataca aacattcctt gttctgataa
     3901 tgtacgtgca ttcgaaatca tttgttcata taattttaac ttagctttta acggtggttg
     3961 cttcttcatt gcgccaaagc caacatttgc gaattcatca gttgttcccc agttgtgtga
     4021 gatatgcata acatcgatat attcagcaat atctaaataa cgatcttgag gcaatgttag
     4081 gtttgaattc atttgtgtat atatacctcg atgatgtgca tactttaata gaggtttaac
     4141 aacatttcta atagactttt tagaaaacat tggttcgcca cctgtaatag acatcgttcg
     4201 cagatttgga atttcatcaa gtctacgata aattaagtcc atatctaaag gctcggggtc
     4261 gacagtttgt aaagtataac caactgcaca gtggctacaa cgcatattac aaagatttgt
     4321 agttgtaaat tcgatgttac ttaaagttaa ttggccatgt tctttaacat cgttatatgc
     4381 ttcccatgga tcattgtaaa tgctaacagg ttgcttcaat ttgacgctat tcatcgtctt
     4441 aaacttcctt ctttctattt ggttcatatc ggtatgatac cacttcgtat tatcactcgt
     4501 aacaaattat ctcttaattt tagttaattg aaaaggggat ttttaaaagt caaaataatt
     4561 tgaatgtcat aaatatagct aagattatat cgctttaacg caatttctgt taggataata
     4621 atgaaaaaga aaacaaaata aaaggagtta actttttatg aatgaacaac aaacaatcga
     4681 acagataaaa gcgcgtttaa ataagtttat tgaggatatc gatcatgtaa atcctgatga
     4741 agtacgtgtt gaagatatag atgaatggat tggattgtta gatcagcttg aagaaaaggt
     4801 taaattagta tctaagtaaa tgccacataa gatgtgttag ggttagatat cgcgacagtc
     4861 gcttaatatt ttttatatct tgtatgttta atcactgatt tacttgtcta tagtaattat
     4921 atcaatgata tatagaaagg tgatttgcaa tgactaaaaa agtagcaatt attctagcaa
     4981 acgaatttga agatatagaa tattcaagcc ctaaagaggc attagagaat gcaggcttta
     5041 atactgtagt gattggagat actgcaaata gtgaagttgt tggtaaacac ggtgaaaaag
     5101 ttactgtcga tgtaggcatt gcagaagcta aaccagaaga ttatgatgca ttattaattc
     5161 ctggaggatt ttcaccagat catttacgtg gagatacaga aggtcgatat ggcacatttg
     5221 ctaaatactt tactaaaaat gatgtaccaa catttgccat ttgtcatggg ccacaaatac
     5281 taatagatac agacgattta aaaggtcgta cgttaacagc agtattaaat gtacgcaaag
     5341 atttatcaaa tgcaggcgca catgtagttg atgagtcagt agttgtagac aacaatattg
     5401 taacaagtcg agtaccagac gatttagatg attttaatcg agaaatcgtt aaacaattac
     5461 aatagaagta ttgtggttat cgattgttaa aatttatata ttttcgtttg aaatgaaata
     5521 tggattttag ttttaataaa tgaataacaa ataaaaggtc gtctctattg gcatttaata
     5581 gggatggtct tttattttta taaaatataa caatatttga catgattaat atttaaaatt
     5641 gcaatgattt atctataatt ttttcaaaaa acaaaaaagt aagtatgatg gcgtatacga
     5701 ttgtaagccc ttggactgat tttcccaaaa agtgtagcct atttgtgata gtactggtaa
     5761 actcaaggta tatactaagt gagttttaaa agaaggagca aacgcatgaa aagaagcgat
     5821 aggtactcaa actcaaatga acattttgag catatgaaac acgaacctca ctataatacg
     5881 tattatcaac cagttggcaa accgccgaaa aagaaaaaaa gtaaacgaat actattaaaa
     5941 atattattaa ccattctaat cattatcgca ttgtttattg gtatcatgta ttttttatct
     6001 acacgcgata atgtggatga actaagaaaa attgaaaata aaagtagttt tgtgtcagct
     6061 gataacatgc cagagtatgt taaaggtgcc tttatttcaa tggaagatga acgattctac
     6121 aatcatcatg gattcgattt gaaaggtaca actagagctt tattttcaac gattagcgac
     6181 agagatgtgc aaggtggtag taccattaca caacaagttg tcaaaaatta tttttatgat
     6241 aatgatcgtt catttactag aaaagtaaaa gaattatttg tagctcatcg agttgaaaaa
     6301 caatataata agaacgaaat tttaagcttt tatttaaata atatttactt tggggataat
     6361 caatatacgc ttgagggcgc agcaaaccat tactttggaa caaccgtgaa taaaaatagt
     6421 acaacaatgt ctcacataac agttttacaa agcgctattt tagctagtaa agtcaatgca
     6481 cctagcgtat ataatatcaa taatatgtca gagaatttta cgcaacgtgt aagcacgaac
     6541 ttagaaaaaa tgaagcaaca aaattatatc aatgaaacac aatatcaaca ggctatgtca
     6601 caattaaatc gttaaatact tgcattcatg tgtaagatgc ttggaactat aatgtagaag
     6661 gtttaattga aatgtcataa tgataaagtt gaattgtttc tgctttataa attttacaga
     6721 tttgcttgta atcacacttt cattttaaaa gatgatagtg tgattttttg ttagcatcga
     6781 aaaatattta aagttaaaca tttgctatag gaagtggctt attgaataat aaaagagtat
     6841 tgtattaaaa cttaagaaag taacggggtg atccagtgcc gaagattact aaaatagaag
     6901 ttcaaaagaa aaacaaagaa cggtttaatc tttttttaga cgaacaattt gaaatgggta
     6961 tagatattga tacattagtc aaatttaatt taaaaaaagg gcaacaactt gaagctgctg
     7021 acatggcaga gattcaaaag tatgatcatt atcgcatagg tttaaataaa gcaatccaat
     7081 atttatcata taaaaagaga actgaaaaag aagttataca atatttacaa aaagaagaga
     7141 tatcagagca agcgatttct gaagtgattg aatattgtta tcgcgaaaag ttaatcgacc
     7201 atcaagatta tgcggaaagt ttaaaaaata caatgattcg cacgacagat aaaggaccta
     7261 aaatttatca acaaaaacta tatcaacttg gtatagaacc aaatatcatt gaaatattta
     7321 cagaacttta tagagaacaa caggaacttg atgatattat ccaaattgct gaaaaaatat
     7381 ctaaaacaaa aaaagggcca caaaataaag ttaaagaaaa agtaatgcaa tctttaattc
     7441 aaaaagggtt tgaaatggaa accattcatg ctgtattgaa tgaaatggat tttacacaag
     7501 atgaagcggt tttagacgat ttattacaac gagatttaga aaaaatttat aataaaaatc
     7561 gaaagaagta cacgcaacag aaattaattt caaaaactat cgaaggcctt atgagaaaag
     7621 gatataaata tgataaaatt aaagctaaat tagaagaaag tggtattgcc gatggaacag
     7681 aagaaattga gtgagatgtc tgagccagaa ttaagacacg aaatacaact ttataaagag
     7741 aaaatgagaa aagctgagat gaatggtatt ttaaatgaat atgacgttta tcaaagtaaa
     7801 gtaattgttg ccgaaagtta cttagtagac agaaaaaaga tagaaattgg taaaatttat
     7861 aaactgactg atggcagtaa tcaatatttt aaagtagaaa gattaaaagg tatttttgcg
     7921 tgggggttta gatttaatag cgatgaaccc gaagaaggat tacctatagc gttattacaa
     7981 ttatagaagg atgaaaatag atgggaagtt caattgtttt aaagttacta aaagtaacac
     8041 actactatag gaataaacag aataagaaat ggtatttacc ttttggatat gatgctgaag
     8101 atatcgattt aaacaatatt agtttacata tttatcaagg agaagcatta ggtataattg
     8161 gtgaacctga atcttccaaa gcgttggtag gtcaattgtt ggcaggtgca attaaacctg
     8221 ataaaggtaa agtagtttgt actgaagatt tgttctacgg atatattgaa gatcaatcgt
     8281 taattcatca aactgttgaa gcttatacag cgcagttagt tcaactattt ccatatgaaa
     8341 ttaatgatca taaagctgaa cagattattc aatatgcaca tttaggtgat tataaaacga
     8401 agccggttaa ccatatttcg aaagcggcat acgctcaatt actattaagt attgcacgct
     8461 catcaaaatc aaatattatt attttaaatc atgttattga ctatttaaca ccacaattta
     8521 tggaacgtgc gattgaatta acaaatgatt atattgaaaa taatttaacg attgtgtcaa
     8581 ttggtgatga tattgataaa atttcacaag tgagtaacta catagcttgg ttttcacatg
     8641 gtcaattaag aatggaaggg tcacttaaac aagttattcc atcttttaaa gaacatgaac
     8701 gtgatcgatt aagtctaaac tcaaaagaag aaattgaaaa ctttgattta gattggaaaa
     8761 agaatcgtac aagaatacca gagatgacct ataatttcaa acgtgttgag cgctataatc
     8821 atgcaaaacc gcctaagttt ttagtgcgtt tttggacttt ggcctcaggt actattttag
     8881 gcttagcatt gatgatgttg ctcattttca ataatatagg aattatttcg ataacagatt
     8941 ttacgaatcg tgctacgatg caaaatgaaa ataaagatcc atatggcgaa aagttagctt
     9001 atggaattgc ttttaatggc agtgtggata tgcaagggga taaacaagtc acaattccaa
     9061 aatatagtgt agttacaatt actggcgaaa atagtaaaaa ttatcgtgtt accgccgata
     9121 ataagactta ctatgttagt aaagataaat tagaatattt taacccggca ggtttatatc
     9181 aaacgcatag ttttaaaaaa ttagcaccat atatgaaatc aaattatagt aattactatg
     9241 catactttaa tagtcaatta cataaaaagc atagttcagt tataaaaact ttagttcctg
     9301 atgatgataa ccgtttcgtt gcatccgtta cacaacaacc gatacaatta cttttcaatg
     9361 ataataatca gttatacggt tttgtttatc caattgtaga taaaaaagaa ttaaaagata
     9421 agtttaatat taacaataac atttggatta ctaaagttgg gaatggatat tgtattgcca
     9481 atttgaaaga agacaaatgg atttatattg aattgtaggt gtaaagatgc tagataatat
     9541 tattttatat tttaaaaact taccgcatac taaaaggtat gtaacagaac gtttaaaaca
     9601 aagttggaaa agttttctaa tcgtattagc tgcatgttta atacttatta ttgcaagtga
     9661 aacgctattt tcctttagtc atttgaccga tgttaaagag gtgagatggt tatttagaat
     9721 cattgtattg attgtttttg cggtagtgat gttcacaatt tatatctctt atcatcatta
     9781 tatgaatgac tttttagtta ctaagttatt taatatttcc gcagcgacgc cagtagttat
     9841 tatgtctatt ttaagtttta ttatgctagt cattttgacg atgattagtg cattggttaa
     9901 accagtaaac ttcgaaacat catatatagc gctattctat tttattgtga tggcaactat
     9961 ttttgtagga ttaatatccg ttacttttgg tcttattcga ctgttaactg aaaaaataaa
    10021 tatcatattt tatggggttt gtgtattgtg tttcttattg ttaccaatta ttttcatacc
    10081 aaatccaaat catgtattta tcaatcacat cctaatgcta aatccaatgt attacattgt
    10141 taatggtata gcacaatcta tcatatttgg tatatcaagt atggaaaaca ttccatatca
    10201 tttttacttt attttattct tatgtttaat agctgcagta aatttcgtat tagcaaggta
    10261 tacgacacac gcaatttata ataaaacatc taaagtgaca caaactgata atcaacagga
    10321 tgtttcaaat gatagtacag acgaggcgga tacttcttca tgatgtttag ctgacctgtt
    10381 gtgtaaatta atctgtgaga atcagaaaca attagaatat catctagata ttgttttcct
    10441 atactgattt tgatatttcg agtcattaaa ggtagtttgt tagcaacaat ataaatttta
    10501 gattttgtaa aagcattcat agttttacat taaaaaaccg gacagagtaa aaatcgtttt
    10561 gattttcatt ctgcccggtt gttcattttt tatagattaa ctttagtgaa taataacttg
    10621 aaattattta gcatacagtt tacgttgtaa cttatcttct gtaaataccc agccaatata
    10681 agagtggtct atgacattat cgtttgttac atgtgcaatt gccacaaatg aataatgatc
    10741 attttttaaa taacgcaaat caatcaaacg aatttcagta gaaccatctg ctaacgttgt
    10801 tgtttgccaa cgatagattg atgaaaagtt taaaaaagta cgtatatctt tattaccttt
    10861 gactttccat aaaatggagt ctgttgataa tgtttgacgt tccactttat cactaaacac
    10921 cacattccta ccatatgctt ttccaacata gtcatgtgca tcagtttgta tcgcgacacg
    10981 ccattccata aattttattg ttggcgcaac aaatacttta acagggtgat gctcttgttg
    11041 aattgctttt aaagcttgtt gcttaattac ggctctcatt ttaaaacgaa tcatgtaata
    11101 aatgattagt aaagcgatta taggaaagaa gactgcaaat ggatgcaagc ctataaccca
    11161 taatacaata ccaatacaaa gaactgtgaa aataataggg tcaaatgtgt taatcacact
    11221 taattgaatc catttgtttg tgataggtct aagcgcttgt gtaccataag aattgaatat
    11281 atctacaaag acatgtaaaa atactgcgag ctgagcccac atccatacat gaaatgggtt
    11341 ggttccacta aagaacgtga atattaaaaa tgtaattaaa attggccata gtattgtgaa
    11401 agggatggaa tgcgtgatac ctctatgatg cgaaatatat gttgcattgt cctttaattt
    11461 aagaacagta tccccatcag gaattaatga accaacgata agggttgtag ctgttgcacc
    11521 aaacgtagaa gccattgctg gatcttgagt tgcaagtgct gtaaggccca ccccaattgc
    11581 gatatgtgtt gcagtgtcca tatcagttca ctcatttcat aatattatat atactattat
    11641 aatccaattt cgcaaataac taaattaact tacatactaa ccatgatttt aatgtacaat
    11701 aaaaacatta tgagtaaaga caattaattt tggtgaggaa agggttttat aaatgtatca
    11761 gcagtctagt tttaaagaaa atttgataca ctggtttgat gaaaatcaaa gagaaatgcc
    11821 atggcgacaa acgaccaatc cgtactatat ttggttaagt gaggtcatgt tacaacagac
    11881 acaagttaaa acagttattg actattatca tcgttttgtt gaacgatttc caactgtgga
    11941 agttttgagt caagcttcgg aagatgaagt gttaaagtat tgggaaggct taggatatta
    12001 tagtagagct cgaaattttc atacggcgat taaagaagtt catgataaat atgaaggctt
    12061 agttcccaaa gatcctgatc aatttaaagc gttaaaaggt gttggcccat acacacaagc
    12121 tgcagtaatg agcattgcat ataatgtacc actagcaact gtagacggaa atgtttttcg
    12181 agtatggtct agattgaatg atgactatcg agatattaaa ttacaatcga cgaggaaatc
    12241 gtacgaacaa gaattattac cgtatgttac tacagaagct ggaacgttta atcaagctat
    12301 gatggaatta ggggcgttaa tttgtacacc aaaaaatcca ttatgcttat tttgtccagt
    12361 tcaagaaaac tgtgaggcat ttgataaagg tacatttgaa aagttacctg ttaaatctaa
    12421 aaatgtaagt aaaaaagtaa ttgagcaatc cgtcttttta attagaaata atcaaggtca
    12481 atatctgttg caaaagcgta gcgaaaaatt attgcatggt atgtggcaat ttcctatgtt
    12541 tgaaagtgag catgctagac gtaaaatgac tgaaaaaatc ggtcatgaca ttcaaccagt
    12601 agaaacacct atttttgagt tgaagcatca atttactcat ttaacatgga agattaaagt
    12661 atacgccgtt tcaggagcaa ttaacattga aacattacca gatgacatga tttggtttga
    12721 tttaagtgat cgagatcaat atacattccc tgtacctatg tcaaagattt atcaatttat
    12781 aaatgggtaa cattcgatga ttggtaatat tatcggaaaa catagtgaca ttaattttaa
    12841 aaagtataaa tgatatcttt aaatctcata gaataacaat gtcaaaaagt taaagtgttg
    12901 atatataaaa gtgatgattt tatatacttt aaatgggaca tgtaaatctc tgttgtagta
    12961 cttagcaata ttaccgagtt taattattaa
//
LOCUS       DAKTIO010000066        12989 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.066, whole genome shotgun sequence.
ACCESSION   DAKTIO010000066 DAKTIO010000000
VERSION     DAKTIO010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12989
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.066"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            178..1119
                     /locus_tag="O0293_001410"
     CDS             178..1119
                     /locus_tag="O0293_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HCY0280299.1"
                     /translation="MNVGIKGFGAYAPEKIIDNAYFEQFLDTSDEWISKMTGIKERHW
                     ADDDQDTSDLAYEASLKAIADAGIQPEDIDMIIVATATGDMPFPTVANMLQERLGTGK
                     VASMDQLAACSGFMYSMITAKQYVQSGDYHNILVVGADKLSKITDLTDRSTAVLFGDG
                     AGAVIIGEVSDGRGIISYEMGSDGTGGKHLYLDKDTGKLKMNGREVFKFAVRIMGDAS
                     TRVVEKANLTSDDIDLFIPHQANIRIMESARERLGISKDKMSVSVNKYGNTSAASIPL
                     SIDQELKNGKIKDDDTIVLVGFGGGLTWGAMTIKWGK"
     gene            1131..2375
                     /gene="fabF"
                     /locus_tag="O0293_001411"
     CDS             1131..2375
                     /gene="fabF"
                     /locus_tag="O0293_001411"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCY0280300.1"
                     /translation="MSQNKRVVITGMGALSPIGNDVKTTWENALKGVNGIDKITRIDT
                     EPYSVHLAGELKNFNIEDHIDKKEARRMDRFTQYAIVAAREAVKDAQLDINENTADRI
                     GVWIGSGIGGMETFEIAHKQLMDKGPRRVSPFFVPMLIPDMATGQVSIDLGAKGPNGA
                     TVTACATGTNSIGEAFKIVQRGDADAMITGGTEAPITHMAIAGFSASRALSTNDDIET
                     ACRPFQEGRDGFVMGEGAGILVIESLESAQARGANIYAEIVGYGTTGDAYHITAPAPE
                     GEGGSRAMQAAMDDAGIEPKDVQYLNAHGTSTPVGDLNEVKAIKNTFGEAAKHLKVNS
                     TKSMTGHLLGATGGIEAIFSALSIKDSKVAPTIHAVTPDTECDLDIVPNEAQDLDITY
                     AMSNSLGFGGHNAVLVFKKFEA"
     gene            complement(2430..2801)
                     /locus_tag="O0293_001412"
     CDS             complement(2430..2801)
                     /locus_tag="O0293_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HCY0280301.1"
                     /translation="MLKKWLGMALITPILTFIIWLFNSHTIITYLNILFYVSLIIFIS
                     IFLILLVQEGIFDATSYGFRRLKYQMSSSKKKKSISDDPFFNPQEVKKEHYFVSTWII
                     PLLLINILYFIMTIVLSLILV"
     gene            3044..3970
                     /locus_tag="O0293_001413"
     CDS             3044..3970
                     /locus_tag="O0293_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0280302.1"
                     /translation="MGKYIFKRFIYMLISLFIIITITFFLMKLMPGSPFNDAKLNAEQ
                     KEILNEKYGLNDPVATQYLHYLKNVVTGDFGNSFQYHNQPVWDLIKPRLLPSFEMGLT
                     AMFIGVILGLILGVAAATKQNSWVDYTTTVISVIAVSVPSFVLAVLLQYVFAVKLRWF
                     PVAGWEGFSTAVLPSLALSAAVLATVARYIRAEMIEVLSSDYILLARAKGNSTMRVLF
                     GHALRNALIPIITIIVPMLASILTGTLTIENIFGVPGLGDQFVRSITTNDFSVIMAIT
                     LLFSTLFIVSIFIVDILYGVIDPRIRVQGGKK"
     gene            3970..5040
                     /locus_tag="O0293_001414"
     CDS             3970..5040
                     /locus_tag="O0293_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0280303.1"
                     /translation="MAENKNNLSINDDHSNAAMTHTSDAIASSDFIIRELDLNQEPEM
                     QRESKNFWQDAWAQLKRNKLAVVGMIGLIIIVIFAFIGPVINKHDYAEQNVEHRNLPA
                     KIPVLDKVPFLPFDGKDADGKDAYKAANAKENYWFGTDQLGRDLWTRTWKGAQISLFI
                     GVVAAMLDIFIGVVYGAISGFFGGRVDTIMQRILEVIASIPNLIVVILFVLIFEPSIW
                     TIILAMSITGWLGMSRVVRGEFLKLKNQEFVMASKTLGASKFKLIFKHILPNTLGAIV
                     VTSMFTVPSAIFFEAFLSFIGIGVPAPQTSLGSLVNDGRAMLLIYPHELFIPAMILSL
                     LILFFYLFSDGLRDAFDPKMRK"
     gene            5057..6139
                     /locus_tag="O0293_001415"
     CDS             5057..6139
                     /locus_tag="O0293_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0280304.1"
                     /translation="MTERILEVNDLHVSFDITAGEVQAVRGVDFYLNKGETLAIVGES
                     GSGKSVTTKAITKLFQGDTGRIKKGEILFLGEDLAKKPENELIKLRGKDISMIFQDPM
                     TSLNPTMQIGKQVMEPLIKHKNYSKAQAKKRALEILNLVGLPNAEKRFKAYPHQFSGG
                     QRQRIVIATALACEPKVLIADEPTTALDVTMQAQILDLMKELQQKIDTAIIFITHDLG
                     VVANIADRVAVMYGGQMVETGDVNEIFYDPKHPYTWGLLSSMPDLSTTNDTPLLAIPG
                     APPDLLHPPKGDAFARRSQYALDIDFKVEPPWFKVSPTHFVKSWLLDARAPKVELPEL
                     VKQRMKPMPNNYEKPLKVERVSFNEK"
     gene            6129..7070
                     /locus_tag="O0293_001416"
     CDS             6129..7070
                     /locus_tag="O0293_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0280305.1"
                     /translation="MKNDEVLLSIKNLKQYFNAGKKNEVRAIENISFDIYKGETLGLV
                     GESGCGKSTTGKSIIKLNDITSGEILYEGIDIQKIRKRKDLLKFNKKIQMIFQDPYAS
                     LNPRLKVMDIVAEGIDIHHLATDKRDRKKRVYDLLETVGLSKEHANRYPHEFSGGQRQ
                     RIGIARALAVEPEFIIADEPISALDVSIQAQVVNLLLKLQRERGITFLFIAHDLSMVK
                     YISDRIAVMHFGKIVEIGPAEEIYQNPLHDYTKSLLSAIPQPDPESERSRKRFSYIDD
                     EANNHLRQLHEIRPNHFVFSTEEEAAQLRENKLVTQN"
     gene            7089..8744
                     /locus_tag="O0293_001417"
     CDS             7089..8744
                     /locus_tag="O0293_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCY0280306.1"
                     /translation="MTRKFRTLILILIATIALSGCANDDGIYSDKGQVFRKILSSDLT
                     SLDTSLITDEISSEVTAQTFEGLYTLGKGDKPVLGVAKAFPEKSKDGKTLKVKLRSDA
                     KWSNGDKVTAQDFVYAWRKTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALN
                     DETLQIELEKPVPYINQLLALNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWK
                     QEDKTLLSKNQYYWDKKNVKLDKVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYK
                     DNKGLNFVLTTGTFFVKMNEKQYPDFKNKNLRLAIAQAIDKKGYVDSVKNNGSIPSDT
                     LTAKGIAKAPNGKDYASTMNSPLKYNPKEARAHWDKAKKELGKNEVTFSMNTEDTPDA
                     KISAEYIKSQVEKNLPGVTLKIKQLPFKQRVSLELSNNFEASLSGWSADYPDPMAYLE
                     TMTTGSAQNNTDWGNKEYDQLLKVARTKLALQPNERYENLKKAEEMFLGDAPVAPIYQ
                     KGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDKSIDKETGKKKK"
     gene            8956..10671
                     /locus_tag="O0293_001418"
     CDS             8956..10671
                     /locus_tag="O0293_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0280307.1"
                     /translation="MGKLIKYISILLIVVLVLSACGKSSNKDEGVKDATKTETSKHKG
                     GTLNVALTAPPSGVYSSLLNSTHADSVVEGYFNESLLATDKKIRPKAYIASWKDIEPA
                     KKIEFKIKKGIKWHDGNELKIDDWIYSIEVLANKDYEGAYYPSVENIQGAKDYHEGKT
                     DHISGLKKIDDYTMQVTFDKKQENYLTGFITGPLLSKKYLSDVPIKDLAKSDKIRKYP
                     IGIGPYKVKKIVPGEAVQLVKFDDYWQGKPALDKINLKVIDQAQIIKAMEKGDIDVAN
                     DATGAMAKDAKSSNAGLKVLSAPSLDYGLIGFVSHDYDKKANKTGKVRPKYEDKELRK
                     AMLYAIDREKWIKAFFNGYASEINSFVPSMHWIAANPKDLNDYKYDPEKAKKILDKLG
                     YKDRDGDGFREDPKGNKFEINFKHYSGSNPTFEPRTAAIKDFWEKVGLKTNVKLVEFG
                     KYNEDLANASKDMEVYFRSWAGGTDPDPSDLYHTDRPQNEMRTVLPKSDQYLDDALDF
                     EKVGIDEKKRKDIYVKWQKYMNDELPGLPMFQGKSITIVNDKVRNLDIEIGTDQSLYN
                     LTKEA"
     gene            10722..11708
                     /locus_tag="O0293_001419"
     CDS             10722..11708
                     /locus_tag="O0293_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0280308.1"
                     /translation="MNNVLLEVKDLETSLKINNEWLATVENISFELSKGEVLGIVGES
                     GCGKSILSKSIIKLLPEKISKLSNGEVIFDGKRIDTLNEKQLPDIRGNDIAMIFQEPM
                     TALNPVFTIKNQLVESIKSHKKISKKEANKLAKDLLKKVGIARQDEILNSYPHQLSGG
                     MRQRVMIAMAISCSPKLLIADEPTTALDVTIQAQILDLLKELQKETQMAIMMITHDLS
                     VVAEFCDKVLVMYAGQIVEFGGIKEILHNPKHPYTQKLLSTIPKLKEEQKRLETIEGI
                     VPSIQAFHVNKCRFANRCNKKLDICNNQSPKMHVCEDVIVRCHLYKNEYKEI"
     gene            11711..12691
                     /locus_tag="O0293_001420"
     CDS             11711..12691
                     /locus_tag="O0293_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCY0280309.1"
                     /translation="MENILEVNQIKKYYKIKTGLLQKTQYVKAVDDVSFSIKKGQTFG
                     LVGESGCGKSTLGKVIIRLEDATSGSIIVNGEDITRLQGKKLRKSRQQYQMIFQDPYA
                     SLNPMQMVGDIISEPILNYKKLPKEEIKKEVLYLLKCVGLSEDAYYKYAHEFSGGQRQ
                     RVGIARALALRPSLIVADEPVSALDVSVQSQVLNLLKDLQEQFNLSYLFIAHDLSVVK
                     HISDVIGVMYLGHIVEIASDKEIYENPKHPYTKALISSIPQIDKHNNNRIILKGELPS
                     PSNPPQGCPFHTRCPIAQDMCKKSMPELKDIGNEHQVACFYVDKVGDLND"
     gene            12684..>12989
                     /locus_tag="O0293_001421"
     CDS             12684..>12989
                     /locus_tag="O0293_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0280310.1"
                     /translation="MIKLILKRLGLMIPLLILISIVVFSLAIIQPGDPFSDLQNGKIK
                     QEAINAQREKLGLNDSIPHQYIRWVNHVIHGDLGESIKYKRPVIDVIEERIPNTILLG
                     "
BASE COUNT         4793 a         1815 c         2470 g         3911 t
ORIGIN      
        1 tgaatggcta ttctaaatta agcgcattaa aaccaatttc atactgaaat ttgacgataa
       61 taaagcatta aaattttatt aactagtcaa tattcctacc tctgacttga gtttaaaaag
      121 taatctatgt taaattaata cctggtatta aaaattttat taagaaggtg ttcaactatg
      181 aacgtgggta ttaaaggttt tggtgcatat gcgccagaaa agattattga caatgcctat
      241 tttgagcaat ttttagatac atctgatgaa tggatttcta agatgactgg aattaaagaa
      301 agacattggg cagatgatga tcaagatact tcagatttag catatgaagc aagtttaaaa
      361 gcaatcgctg acgctggtat tcagcccgaa gatatagata tgataattgt tgccacagca
      421 actggagata tgccatttcc aactgtcgca aatatgttgc aagaacgttt agggacgggc
      481 aaagttgcct ctatggatca acttgcagca tgttctggat ttatgtattc aatgattaca
      541 gctaaacaat atgttcaatc tggagattat cataacattt tagttgtcgg tgcagataaa
      601 ttatctaaaa taacagattt aactgaccgt tctactgcag ttctatttgg agatggtgca
      661 ggtgcggtta tcatcggtga agtttcagat ggcagaggta ttataagtta tgaaatgggt
      721 tctgatggca caggtggtaa acatttatat ttagataaag atactggtaa actgaaaatg
      781 aatggtcgag aagtatttaa atttgctgtt agaattatgg gtgatgcatc aacacgtgta
      841 gttgaaaaag cgaatttaac atcagatgat atagatttat ttattcctca tcaagctaat
      901 attagaatta tggaatcagc tagagaacgc ttaggtattt caaaagacaa aatgagtgtt
      961 tctgtaaata aatatggaaa tacttcagct gcgtcaatac ctttaagtat cgatcaagaa
     1021 ttaaaaaatg gtaaaatcaa agatgatgat acaattgttc ttgtcggatt cggtggcggc
     1081 ctaacttggg gcgcaatgac aataaaatgg ggaaaatagg aggataacga atgagtcaaa
     1141 ataaaagagt agttattaca ggtatgggag ccctttctcc aatcggtaat gatgtcaaaa
     1201 caacatggga gaatgctcta aaaggcgtaa atggtatcga taaaattaca cgtatcgata
     1261 ctgaacctta tagcgttcac ttagcaggag aacttaaaaa ctttaatatt gaagatcata
     1321 tcgacaaaaa agaagcgcgt cgtatggata gatttactca atatgcaatt gtagcagcta
     1381 gagaggctgt taaagatgcg caattagata tcaatgaaaa tactgcagat cgaatcggtg
     1441 tatggattgg ttctggtatc ggtggtatgg aaacatttga aattgcacat aaacaattaa
     1501 tggataaagg cccaagacgt gtgagtccat ttttcgtacc aatgttaatt cctgatatgg
     1561 caactgggca agtatcaatt gacttaggtg caaaaggacc aaatggtgca acagttacag
     1621 catgtgcaac aggtacaaat tcaatcggag aagcatttaa aattgtgcaa cgcggtgatg
     1681 cagatgcaat gattactggt ggtacagaag caccaattac tcatatggca attgctggtt
     1741 tcagtgcaag tcgagcgctt tctacaaatg atgacattga aacagcatgt cgtccattcc
     1801 aagaaggtag agatggtttt gttatgggtg aaggtgctgg tattttagta attgaatctt
     1861 tagaatcagc acaagctcga ggtgccaata tttatgctga gatagttggc tatggtacta
     1921 caggtgatgc ttatcatatt acagcgccag ctccagaagg tgaaggtggt tctagagcaa
     1981 tgcaagcagc tatggatgat gctggtattg aacctaaaga tgtacaatac ttaaatgccc
     2041 atggtacaag tactcctgtt ggtgacttaa atgaagttaa agctattaaa aatacatttg
     2101 gtgaagcagc taaacactta aaagttaact caacaaaatc aatgactggt cacttacttg
     2161 gtgcaacagg tggaattgaa gcaatcttct cagcgctttc aattaaagac tctaaagtcg
     2221 caccgacaat tcatgcggta acaccagata cagaatgtga tttggatatt gttccaaatg
     2281 aagcgcaaga ccttgatatt acttatgcaa tgagtaatag cttaggattc ggtggacata
     2341 acgcagtatt agtattcaag aaatttgaag cataactata aaaatcttca gtaacgttgt
     2401 tttagttact gaagattttt tcagtttctt tatactaaga tgagcgacag cacaatcgtc
     2461 ataataaaat ataaaatatt tattaataat aaggggatta tccatgtaga aacaaagtaa
     2521 tgctcttttt ttacctcttg tgggttgaaa aatggatcat cagagataga cttcttcttt
     2581 ttcgaagatg acatttgata ctttaatctt ctaaaaccat aacttgtcgc atcaaaaatg
     2641 ccttcttgta caagtaaaat caaaaatatg ctaataaaaa taattaatga aacataaaac
     2701 aatatattta aatatgtaat gatagtatgg ctattaaaaa gccatataat aaacgttaat
     2761 attggcgtta ttagtgccat tccaagccat tttttcaaca tttgatcact cccacttata
     2821 gaaaactctt acgcatagtt tacattaaaa tcagacattg aggaatgatt ttttaatttc
     2881 ttcagcttta ttgaaattct aaaatcaatc attcttcatt agtttaaagc aaaaaaatat
     2941 tgatatatag taaatattgt atatataata ttagttaaga tttcagaaaa ttttgaaggg
     3001 aatggaaatt tagaaatcgg aatttgttag aggaggggat tagatgggga aatatatttt
     3061 caaacgattt atttatatgc ttatttcttt atttattatt attacaatta catttttctt
     3121 aatgaaatta atgccaggtt cgccatttaa cgatgctaaa ttaaatgctg aacaaaaaga
     3181 aattttaaat gaaaaatatg gattaaatga tcctgtagct acgcagtatt tacattattt
     3241 aaaaaatgtt gttacaggcg attttggtaa ttcattccag tatcataatc aacctgtgtg
     3301 ggatttgatt aaaccgagac tactaccttc ttttgaaatg ggtcttacag caatgttcat
     3361 cggtgtgata ctgggactta ttttaggtgt tgcagcagct actaaacaaa attcttgggt
     3421 tgactataca actacagtta tttcagttat tgcagtatct gtaccatctt ttgtacttgc
     3481 tgtactttta caatatgtat ttgcagttaa attaagatgg ttcccagtag ctggatggga
     3541 aggtttttcg accgcggtat taccgtcact tgcattatct gcagctgttt tagcaactgt
     3601 cgccagatac ataagagcag agatgataga ggtattaagt tcagactata ttttattagc
     3661 gagagctaaa ggtaattcga caatgcgtgt actttttgga catgcactta gaaatgcttt
     3721 aattccaatt attacaatta tcgttcccat gttagcaagt attttaacag gcactttaac
     3781 aattgaaaat atttttggag ttcctggatt aggggatcaa ttcgtacgtt caattacaac
     3841 aaatgatttc tcagtaatca tggcaatcac actattattt agcacactgt ttatcgtttc
     3901 tatttttatt gtagatattt tgtacggtgt gatagatcca cgaattcgtg ttcaaggagg
     3961 taaaaaataa tggctgaaaa taaaaacaat ttgtcgatta acgacgatca ttctaatgca
     4021 gctatgacgc atacctctga cgctatcgca tcatctgatt ttattattag agaattagat
     4081 ttgaatcagg aacctgaaat gcaacgagaa agcaaaaact tttggcaaga tgcttgggct
     4141 cagttaaaac gaaataagtt agctgttgtc ggtatgatag gtttaattat cattgtaata
     4201 tttgctttta tcggtccagt tataaataaa catgattatg ctgaacaaaa tgtagaacat
     4261 agaaatcttc cggcaaaaat acctgtatta gacaaagttc catttttacc ttttgatggt
     4321 aaagatgcag atggcaagga tgcttataaa gcagcaaatg ctaaagaaaa ttattggttt
     4381 ggtactgatc agttgggtcg agatttatgg acaagaacat ggaaaggtgc tcaaatttca
     4441 ttgtttatcg gtgttgttgc agcgatgtta gatattttta ttggtgttgt atatggtgcg
     4501 atttctggat tcttcggtgg acgtgtcgat acgattatgc aacgtatact tgaagtcata
     4561 gcatctattc cgaatttaat tgtcgtaatt ttatttgtat taatttttga accatccatt
     4621 tggacaatta tattggctat gtctatcaca ggctggttag gcatgagcag agttgtacgt
     4681 ggagaatttt taaaattaaa aaatcaagag tttgtcatgg cttcgaaaac attgggggct
     4741 tcaaaattca aattgatatt taagcatatt ttacctaata cattaggtgc tatcgtggtt
     4801 acatcaatgt ttacagtacc tagtgctatt ttcttcgaag catttttaag tttcattggt
     4861 ataggtgtac ccgcacctca aacatcgtta gggtcattag taaatgatgg gcgcgcaatg
     4921 ttattaattt atccacatga attatttata ccagcaatga ttttaagttt attaattcta
     4981 ttcttttact tatttagtga tggattacgt gatgcatttg atccgaaaat gcgtaaataa
     5041 aaagggggca tagcatatga ctgaaagaat attagaagta aatgatttgc atgtttcctt
     5101 tgatattaca gcaggggaag tgcaggcagt gagaggcgta gatttttatt tgaacaaagg
     5161 ggaaacattg gcaattgttg gtgaatcagg ttcaggtaaa tctgtaacaa caaaagcaat
     5221 tacaaaatta ttccaagggg acacaggaag aattaaaaag ggagaaattt tatttttagg
     5281 ggaagattta gcaaaaaaac ctgaaaatga gttgattaaa ttacgtggca aagatatttc
     5341 aatgatcttt caagatccaa tgacatcttt aaacccaacg atgcaaattg gtaaacaagt
     5401 catggaacca ttaattaagc acaaaaatta tagtaaagca caagctaaaa agcgcgcatt
     5461 ggaaatacta aatcttgtag gtttaccaaa tgcagaaaaa agatttaaag catatcctca
     5521 tcaattttca ggtggacaaa ggcaaagaat tgttattgca accgcattag cttgtgaacc
     5581 taaagtgctc attgctgatg aaccaacgac tgcattagac gtaacgatgc aggcacaaat
     5641 tttagattta atgaaagaac tacaacaaaa aatcgataca gcaattattt ttataacgca
     5701 tgatttaggg gttgttgcga atattgctga tagagtggca gttatgtatg gtggtcaaat
     5761 ggttgaaaca ggagatgtta acgaaatatt ttatgatcca aagcatccat atacatgggg
     5821 attattatcg tcaatgcctg atttatcaac aacaaatgac acaccattac tagcgattcc
     5881 tggagcgcca cctgatttat tacacccacc taaaggtgat gcatttgcga gacgtagtca
     5941 atatgcatta gatattgatt ttaaagtaga accaccgtgg tttaaagttt caccgacaca
     6001 ttttgtgaaa tcttggttat tagacgcacg tgcaccaaaa gttgaactac ccgagctggt
     6061 aaaacaacgt atgaaaccga tgcctaataa ttatgaaaaa ccactcaagg tagaaagggt
     6121 gtcgttcaat gaaaaatgat gaagtgctat tatctattaa aaatttaaag caatatttta
     6181 acgcaggaaa gaaaaacgaa gtgagagcga ttgaaaatat ttcgtttgat atatacaaag
     6241 gggaaacatt aggtttagta ggagaatcgg ggtgtggtaa atctacaact ggtaaatcaa
     6301 ttattaaact taatgatatt acaagtggag aaattttgta tgagggtatt gatatacaaa
     6361 agattcgtaa acgtaaagat ttgcttaaat ttaataaaaa gatacagatg atttttcaag
     6421 acccatatgc gtctttaaat cctaggttaa aagtaatgga tatagtagct gaaggtattg
     6481 atatccatca tttagcaact gataagcgtg accgaaaaaa acgtgtctat gatttacttg
     6541 aaactgttgg attaagtaaa gaacatgcca atcgctatcc tcatgaattt tcaggtggac
     6601 aacgccaacg tattggaatt gcccgtgcat tagccgttga accagaattc attatcgcgg
     6661 acgaaccaat atcggcattg gatgtttcaa tccaagctca agtagttaat ttattattaa
     6721 aattacaacg tgaaagaggg attacgttcc tatttatagc tcatgatcta tcaatggtga
     6781 agtatatttc agatcgtatt gcagtcatgc attttgggaa aatagttgaa attggaccgg
     6841 cagaagaaat ttatcaaaat ccattacacg attatactaa gtctttatta tcagccattc
     6901 cacaacctga tcctgaatca gaacgcagtc gcaaacgatt tagttatatt gatgatgaag
     6961 caaataatca tttaagacaa ttacatgaaa ttagaccgaa tcactttgtc tttagtactg
     7021 aagaagaagc ggcacaacta cgagaaaata aattggtgac acaaaattaa ggggaagggg
     7081 gaaatgcaat gacgagaaaa tttagaacac ttattttaat tttgattgct acaattgcat
     7141 taagtggttg tgctaatgac gatggtattt attcagataa aggtcaagta ttcagaaaaa
     7201 ttttgtcatc agacttaaca tcccttgata catcattaat aacggatgaa atatcttctg
     7261 aagtgactgc gcaaacattc gaaggtttat acacattagg aaaaggtgac aaaccggtgt
     7321 taggtgttgc gaaagctttt cctgaaaaga gtaaagatgg taaaacttta aaggttaaat
     7381 taagaagcga tgctaaatgg agcaatggtg acaaagtgac tgcacaagac tttgtttatg
     7441 cttggagaaa aacagttgac cctaaaacag gttctgaatt tgcatacatt atgggggaca
     7501 ttaaaaatgc gagtgatatt agtactggta agaaacctgt agagcaatta ggtatcaaag
     7561 cattaaatga tgaaacatta caaattgaat tagaaaagcc ggttccatat attaatcaat
     7621 tattagcact taataccttt gcacctcaaa atgaaaaagt tgccaaaaaa tatggtaaaa
     7681 attacggtac ggcagctgat agagcggtat acaatggtcc atttaaagtt gatgattgga
     7741 aacaagaaga taaaacctta ctatctaaaa atcagtatta ttgggataaa aagaatgtaa
     7801 aattagataa agtgaattat aaagttatta aagacttaca agccggtgca tcattgtatg
     7861 atactgaatc agtagatgac gcagttatta ctgcagatca agtaaataaa tataaagaca
     7921 acaaaggatt aaactttgtg ttaacgactg ggacattttt tgtaaaaatg aatgaaaaac
     7981 aatatcctga ttttaaaaac aaaaatttaa gattggctat cgcacaagca atagataaaa
     8041 aaggatacgt tgattcagtg aaaaacaatg gctcaattcc ttccgataca ctaacagcca
     8101 aaggaattgc gaaagcgcct aatggcaaag attatgcgag taccatgaat tcgcctttaa
     8161 aatataatcc taaagaagca agagcgcact gggacaaagc taaaaaagag ttaggtaaaa
     8221 atgaagtgac attttcaatg aacacagaag atacaccaga tgcaaaaata tctgctgaat
     8281 atatcaaatc gcaagttgag aaaaatttac caggagttac tttgaaaatt aagcaattac
     8341 cgtttaaaca aagagtatca ctagaactga gtaacaattt tgaagcatca cttagtggtt
     8401 ggtctgcaga ttaccctgat cctatggctt atttagaaac aatgaccaca ggtagcgcac
     8461 aaaataatac agactggggt aataaagaat atgatcaatt acttaaagta gcaagaacca
     8521 aattggcact tcaaccgaac gaacgatatg aaaacttgaa aaaagcagaa gaaatgttcc
     8581 taggagatgc accggtagca ccaatttatc aaaaaggtgt tgcacattta acaaatcctc
     8641 aagtaaaagg attaatttac cataaatttg gtccaaataa ctcacttaaa catgtatata
     8701 ttgataaatc gatagataaa gaaacaggta agaagaaaaa ataatatgct ttgtaaatta
     8761 ggctggagac atatctccag tctttttgtg ttggataaaa actttgggaa taaaaattta
     8821 aaataagtcg ttttttaaat tactgaaatt gattaaatgc ataaataact gaatattcta
     8881 aaaataaact tgtaataatt ttttctatga gtaaactaaa aagaaaaaat tagattgaaa
     8941 gtaggaggca tatgtatggg gaagctaatt aaatatattt caatacttct tattgtcgtt
     9001 ttagtgttga gtgcttgcgg aaaaagcagt aataaagatg aaggagtaaa agatgctact
     9061 aaaacggaaa cctcaaaaca taaaggtggt accttaaatg tagcattaac agcaccgcca
     9121 agtggtgttt attcttcgtt attaaatagt acacatgcag attctgtagt tgagggatat
     9181 tttaacgaaa gcttattagc aactgataaa aaaatacgtc ctaaggcata tattgcttca
     9241 tggaaggaca tcgagccggc taagaaaata gaatttaaaa ttaaaaaagg tattaaatgg
     9301 catgatggta atgaattgaa aattgatgat tggatttatt caattgaagt cttagctaac
     9361 aaggactacg aaggtgctta ttatccaagt gtagaaaata tccaaggtgc gaaagattat
     9421 catgaaggaa aaactgatca tattagcgga ttgaagaaaa tagatgacta cactatgcag
     9481 gttacatttg ataaaaaaca agaaaattac ttaacaggat ttattactgg acctttatta
     9541 agtaaaaaat atttatcaga tgtaccaatt aaagatttag cgaaatcaga taaaatccga
     9601 aaatatccta ttggtattgg accgtataaa gttaagaaaa tcgttccagg tgaggctgtt
     9661 caactcgtta aatttgatga ttattggcaa ggtaagcctg cactagacaa aatcaattta
     9721 aaagttattg atcaagcgca aattattaag gcaatggaaa aaggcgatat tgatgttgcg
     9781 aatgatgcta ccggtgcaat ggcaaaagat gctaagtcat ctaatgctgg tctcaaggta
     9841 ttatctgcgc caagcttaga ctacggttta ataggattcg tatctcatga ttacgataaa
     9901 aaagctaata aaactggtaa agtgagacca aaatatgaag acaaagaatt acgtaaagca
     9961 atgctttatg caattgatag agaaaaatgg atcaaagcgt ttttcaatgg ttacgctagt
    10021 gaaatcaata gttttgtacc atctatgcat tggatagcag ccaatcctaa ggacctaaat
    10081 gattacaaat atgatcctga aaaagctaaa aaaatcttag ataagttagg ttataaagat
    10141 agagatggtg acggatttag agaagatcct aaaggtaata aatttgagat taactttaaa
    10201 cattattcag gttctaatcc tacttttgaa ccaagaactg ctgcgataaa agatttctgg
    10261 gaaaaagttg gcttgaaaac aaatgtgaag ttagtagaat tcggtaaata taatgaagac
    10321 ttagcaaatg catctaaaga tatggaagtg tacttcagat catgggcagg aggtacagat
    10381 ccagatccat cagatttata ccacactgat agacctcaaa atgaaatgag aacagtttta
    10441 ccaaaatcag atcaatattt agatgatgca ttagacttcg aaaaagtagg cattgatgaa
    10501 aagaaacgta aagatattta tgttaaatgg caaaaatata tgaatgatga gttacctgga
    10561 ttaccaatgt tccaaggtaa atcgataact attgttaacg ataaagtacg aaacttagac
    10621 attgaaattg gaactgatca aagtttatat aatttaacta aagaagctta gtaatgtaac
    10681 ctcgacgatt ttgtcgaggt ttacttatat ggggggatgt tatgaataat gtattgttag
    10741 aggttaaaga tttagaaaca tcattaaaaa taaataatga atggttagca actgttgaaa
    10801 atatttcttt tgaattatct aaaggagaag ttttgggtat agtaggggaa tctggttgcg
    10861 gtaagtccat attaagtaag tcaattatta aattattacc agaaaagata tctaaactaa
    10921 gtaatggaga agttatattt gatggtaaac gaatcgatac gctcaatgag aagcaattgc
    10981 cagatattcg aggaaatgat attgctatga tttttcaaga acctatgact gctttaaatc
    11041 ctgtatttac cataaaaaat caacttgtgg aatctataaa atcacataaa aaaatttcta
    11101 aaaaagaagc aaataaatta gcaaaagatt tactaaaaaa agttggaatt gctagacaag
    11161 atgaaatatt aaatagctat cctcatcaat tatctggtgg tatgagacaa agagtaatga
    11221 ttgcaatggc catttcatgt tctcctaaat tattaattgc tgatgaacct acaacagcat
    11281 tggatgtcac gattcaagcg caaatattag acttattaaa agaattgcaa aaggaaacgc
    11341 aaatggcaat tatgatgatt acacatgatt tgagtgtagt tgctgagttt tgcgataaag
    11401 tcttagttat gtatgcaggt caaattgtag aatttggagg cataaaagaa atactacaca
    11461 atccgaaaca tccttatacc caaaaattat tatcaacaat tccaaaactt aaagaagagc
    11521 agaaacgact tgaaacgata gaaggaattg tgccatcaat ccaagcattt cacgttaata
    11581 agtgcagatt tgcaaataga tgtaacaaaa aactggatat ttgtaataat caatctccta
    11641 aaatgcatgt ttgtgaagat gtcattgtac gttgtcattt gtacaaaaat gaatataagg
    11701 agatataaga atggaaaata ttttagaagt caaccaaata aaaaaatact acaaaattaa
    11761 aactggatta ttacaaaaaa ctcagtacgt taaagctgtt gatgacgtat cgttttcaat
    11821 aaaaaaagga caaacttttg gattagtagg agaatcgggt tgtggtaagt caacgttagg
    11881 taaagtgatt atcaggcttg aagatgcaac ttcaggctca ataattgtta atggtgaaga
    11941 tataacaaga ttacaaggta aaaaactcag aaaatcacga caacaatatc agatgatatt
    12001 tcaagatccg tatgcatcat tgaatccgat gcaaatggtt ggagatatca tttcagaacc
    12061 tattttaaat tataaaaaat tgccaaaaga agaaataaaa aaagaagtac tatatttatt
    12121 aaaatgtgtt ggcctaagtg aagatgcata ttataaatat gcacatgaat tttcaggtgg
    12181 acagagacaa agagtgggaa ttgcaagagc attggctttg cgtccgagtt taattgttgc
    12241 tgatgagcct gtaagtgcat tagatgtatc tgttcaatct caagtactga atttattaaa
    12301 agatttacaa gaacaattta acttaagcta tttatttatc gcacatgatt taagtgtagt
    12361 aaaacatata agtgatgtca ttggagttat gtatttaggt catatagttg aaatcgcatc
    12421 tgataaagaa atttatgaaa atcccaaaca tccatataca aaagcgttga tttcatcaat
    12481 accacaaatt gataaacata ataacaatag aattatatta aaaggagaat taccttcgcc
    12541 aagtaatccg ccgcaaggtt gtccttttca tacaagatgt ccaattgctc aagatatgtg
    12601 caaaaaaagt atgccagaat taaaggacat tggtaatgaa catcaagttg cttgtttcta
    12661 cgtagataaa gtaggtgatt taaatgatta aattaatact taaaagatta gggttaatga
    12721 tcccgttact aattttaatc tcaatagttg tattttcatt agcaatcata caaccaggag
    12781 atccattttc agatctacaa aacggtaaaa taaaacaaga agcaataaat gcacaaaggg
    12841 aaaaattagg tctcaacgac tcaataccac atcaatacat tagatgggtc aatcatgtta
    12901 tacacggtga tttaggtgag tcaatcaaat ataaaaggcc ggtaattgat gttattgagg
    12961 aaagaattcc aaatacaata ttactcggt
//
LOCUS       DAKTIO010000067        12828 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.067, whole genome shotgun sequence.
ACCESSION   DAKTIO010000067 DAKTIO010000000
VERSION     DAKTIO010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12828
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.067"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            2..706
                     /locus_tag="O0293_001422"
     CDS             2..706
                     /locus_tag="O0293_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCY0280311.1"
                     /translation="MSKNINQANSKLNTSNKKLKQAYSKSDSKNMKVIYMPHWLEFYS
                     IAIHSDVTSNKKRYYKSYSRGTVVYVKLGSNIGSEFSGNHFCVILDNKDNKGKETVTI
                     VPLSSKGNKNYLKLNESVLNLTATDLKKQIIDISSKVQALAQKYREIDIKLSTEDKKL
                     LSNLNQKANELYRVIGVYSKHKGKDTYVNISAITTISKRRISKINDSDPTGTIIISED
                     DMNEIEKQLKIKFFSN"
     gene            1099..1635
                     /locus_tag="O0293_001423"
     CDS             1099..1635
                     /locus_tag="O0293_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280312.1"
                     /translation="MHNNNEFNKKLKDFIGSDKRMALVKGYVNEYKLTTVLRALNNSD
                     YTKGTIHTRSIGNLKQIVDNRIIPKNITQNTIFKLSNLTLEVNLYERNTIYHGDFAIY
                     YPVESALIIETDTPRLINHINNNPVQKIFIITTNDWSFNTLELEKIVDETIIYDLKQE
                     DPKQYEILYKNKHGKLPY"
     gene            complement(1748..3289)
                     /gene="guaA"
                     /locus_tag="O0293_001424"
     CDS             complement(1748..3289)
                     /gene="guaA"
                     /locus_tag="O0293_001424"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCY0280313.1"
                     /translation="MEMAKEQELILVLDFGSQYNQLITRRIREMGVYSELHDHEISIE
                     EIKKMNPKGIILSGGPNSVYEEGSFTIDPEIYNLGIPVLGICYGMQLTTKLLGGKVER
                     ANEREYGKAIINAKSDELFAGLPAEQTVWMSHSDKVIEIPEGFEVIADSPSTDYAAIE
                     DKKRRIYGVQFHPEVRHTEYGNDLLNNFVRRVCDCRGQWTMENFIEIEIEKIRQRVGD
                     RRVLCAMSGGVDSSVVAVLLHKAIGDQLTCIFVDHGLLRKGEGDMVMEQFGEGFNMNI
                     IRVNAKDRFMNKLKGVSDPEQKRKIIGNEFVYVFDDEASKLKGVDFLAQGTLYTDVIE
                     SGTKTAQTIKSHHNVGGLPEDMEFELIEPINTLFKDEVRKLGIELGIPEHLVWRQPFP
                     GPGLGIRVLGEITEDKLEIVRESDAILRQVIREEGLEREIWQYFTVLPNIQSVGVMGD
                     YRTYDHTVGIRAVTSIDGMTSDFARIDWEVLQKISSRIVNEVDHVNRVVYDITSKPPS
                     TIEWE"
     gene            complement(3314..4780)
                     /gene="guaB"
                     /locus_tag="O0293_001425"
     CDS             complement(3314..4780)
                     /gene="guaB"
                     /locus_tag="O0293_001425"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCY0280314.1"
                     /translation="MWESKFAKESLTFDDVLLIPAQSDILPKDVDLSVQLSDKVKLNI
                     PVISAGMDTVTESKMAIAMARQGGLGVIHKNMGVEEQADEVQKVKRSENGVISNPFFL
                     TPEESVYEAEALMGKYRISGVPIVDNKEDRNLVGILTNRDLRFIEDFSIKIVDVMTQE
                     NLITAPVNTTLEEAEKILQKHKIEKLPLVKDGRLEGLITIKDIEKVIEFPNAAKDEHG
                     RLLVAAAIGISKDTDIRAQKLVEAGVDVLVIDTAHGHSKGVIDQVKHIKKTYPEITLV
                     AGNVATAEATKDLFEAGADIVKVGIGPGSICTTRVVAGVGVPQITAIYDCATEARKHG
                     KAIIADGGIKFSGDIIKALAAGGHAVMLGSLLAGTEESPGATEIFQGRQYKVYRGMGS
                     LGAMEKGSNDRYFQEDKAPKKFVPEGIEGRTAYKGALQDTIYQLMGGVRAGMGYTGSH
                     DLRELREEAQFTRMGPAGLAESHPHNIQITKESPNYSF"
     gene            complement(4818..6086)
                     /locus_tag="O0293_001426"
     CDS             complement(4818..6086)
                     /locus_tag="O0293_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HCY0280315.1"
                     /translation="MKNLILSVQHLLAMYAGAILVPIIVGTSLKFTPEQIAYLVTVDI
                     FMCGVATFLQANKVTGTGLPIVLGCTFTAVAPMILIGQTKGIDVLYGSLFLSGILVII
                     IAPFFSHLVKFFPPVVTGSVVTIIGINLMPVAMNYLAGGQGAKDYGDVKNILLGLMTL
                     IIILLLQRFTTGFIKSIAILIGLVLGTIGAGLLGMVDINQVNHAGWLGIPVPFRFSGF
                     SFDVTSTLVFFIVAIVSLIESTGVYHALSEITGKKLERKDFRKGYTAEGLAIVLGSIF
                     NSFPYTAYSQNVGLVSLSGAKKNNVIYGMVVLLLICGCIPKLGALANIIPLPVLGGAM
                     IAMFGMVMAYGVSILGHIDFKNQNNLLIIAVSVGLGTGISAVPQAFKGLGEQFAWLTQ
                     NGIVLGAISAIILNFFFNGIKYKQTEENVK"
     gene            complement(6086..6664)
                     /gene="xpt"
                     /locus_tag="O0293_001427"
     CDS             complement(6086..6664)
                     /gene="xpt"
                     /locus_tag="O0293_001427"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCY0280316.1"
                     /translation="MELLGQKVKEDGVVIDEKILKVDGFLNHQIDAKLMNEVGRTFYE
                     QFKDKGITKILTIEASGIAPAIMAALHFDVPCLFAKKAKPSTLTDGYYETSIHSFTKN
                     KTSTVIVSKEFLSEEDTVLIIDDFLANGDASLGLYDIAQQANAKTAGIGIVVEKSFQN
                     GHQRLEEAGLTVSSLCKVASLEGNKVTLVGEE"
     regulatory      complement(6895..6997)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            7177..7584
                     /locus_tag="O0293_001428"
     CDS             7177..7584
                     /locus_tag="O0293_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCY0280317.1"
                     /translation="MADITVVNDTGELYNVINQKKSEGYLESELTIISKSKLHLNDLH
                     DSEISLISTSGTFSDRMTKLLTGEDGEHAVLSRYNLAPDELEKYKQLILDDKMLVVAV
                     RDKSSHQEVHENNSAYEEIDITHFAEASKGPKA"
     gene            7727..8389
                     /locus_tag="O0293_001429"
     CDS             7727..8389
                     /locus_tag="O0293_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280318.1"
                     /translation="MEFKTIQSVKDPHYNEALKLYDTKLNLSLTEDKRIIKRSLENNK
                     TENDYVFIVGLENDVVVSLATAHYEATTNSAFLIYLFAKDLPNHDELMTQTLHQIEHE
                     LNLLSNLVHSRSINFLMMEVPKEPADLTQDNGLIIEHRRQFLYEHQFERQTEIDYIHP
                     TNDGHTKKVDLFLKKNIKLTKDIYGTSVKSNYILKYVFANGIAREIIYALLEEMDLRK
                     PF"
     gene            8507..9463
                     /locus_tag="O0293_001430"
     CDS             8507..9463
                     /locus_tag="O0293_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280319.1"
                     /translation="MLTKEFAQRVELSEKQVRKIVQHLEERGYQLSKTEYRGREATDF
                     KEEDIELFKDIADKVKQTNSYDLAFDELEKEKDFLQVIVKNDDKNLPTNQNVAQLVED
                     LRLEIQKMREERHLLGQMMNQVHQQQQELKELQNQLTSKIDSNSESLKAIQTSQEAIQ
                     EAQASQAKALAESTNKVEKNAVTEDKADSKDSKVAGVNTSTDAKTDTKAENAGDGTAT
                     KVDKEDQISATEAIEKASVEQSKNDNAAETSNKEATVDAEAQHDAEQQVAEAHAEASK
                     QATSNDSLEAKAENDSTASQSEMSEPKPQEEKKGFFARLFNL"
     gene            complement(10254..10355)
                     /locus_tag="O0293_001431"
     CDS             complement(10254..10355)
                     /locus_tag="O0293_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280320.1"
                     /translation="MQLNVNMMKFDRWHKGICKQIVLNSVVIGGKGK"
     gene            10440..11828
                     /locus_tag="O0293_001432"
     CDS             10440..11828
                     /locus_tag="O0293_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HCY0280321.1"
                     /translation="MNAFLTLINIIVLVIFIVILHMMARKHISFAKRVFTALGIGIVF
                     GVLLHLIYGTHSNVITSTSDWFNIVGQGYVALLQMIVMPLIFISIVAAFTKIQIGEKF
                     AKIGSLIFIFLIGTVTIAAIVGVVYALVFGLDASTINLGNAEQARGSEIAKQAKDLTA
                     HTLPQQILELLPKNPFLDFTGQRATSTIAVVIFASFIGFAYLRVARKQPDHGELLKRA
                     IDAIYSLVMAIVTFVLRLTPYGVLAIMANTLSTSDFGAIWTLGKFLIASYAALITMYI
                     IHLIILSLLGISPIRYVKKTLEVLIFAFTSRSSAGALPLNVQTQTRRLGVPEGIANFA
                     ATFGLSIGQNGCAGIYPAMLAIMVAPVANVEIDLQFIVTLIAVVIISSFGVAGVGGGA
                     TFASILVLSTLNLPVALAGVLISVEPLIDMGRTALNVNDSMLAGTGTAKLTKHWDKDT
                     FDSNDNAALTSH"
     gene            complement(11909..12664)
                     /locus_tag="O0293_001433"
     CDS             complement(11909..12664)
                     /locus_tag="O0293_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HCY0280322.1"
                     /translation="MSEHVYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFL
                     QAYSIIGIDDEKIKENLREVSGQPYVVENGYLFVFVIDYYRHHLVDQHAETDMENAYG
                     STEGLLVGAIDAALVAENIAVTAEDMGYGIVFLGSLRNDVERVREILDLPDYVFPVFG
                     MAVGEPADDENGAAKPRLPFDHVFHHNKYHADKETQYAQMADYDQTISEYYDQRTNGN
                     RKETWSQQIEMFLGNKARLDMLEQLQKSGLIQR"
BASE COUNT         4293 a         2430 c         2007 g         4098 t
ORIGIN      
        1 aatgtctaaa aatatcaatc aagcaaatag taaattaaat acgtcaaaca aaaaactaaa
       61 acaagcttat tcaaaatccg acagtaaaaa catgaaagtt atatacatgc ctcattggct
      121 agagttttat tcaattgcta tacatagcga tgttacaagc aataaaaaaa gatattataa
      181 atcatatagt agaggaacag tggtatatgt aaaactaggg agtaatattg gaagtgagtt
      241 ttctggtaat catttttgtg taatattaga taacaaagac aataaaggaa aagaaacagt
      301 tacaatagtt ccactttctt ccaaaggtaa taaaaattat ttgaagttaa acgaatcggt
      361 gctaaatcta acagcaacag atttaaaaaa acagattatc gatatcagta gtaaagtaca
      421 agcattagcc caaaaatata gagaaattga cattaaactt agcacagaag ataaaaagct
      481 tttgtctaac ttaaatcaaa aagctaatga actgtataga gtgataggtg tttattccaa
      541 gcacaaaggg aaagatacat acgttaatat atcagcaatt acaacaataa gcaaaagacg
      601 tataagcaaa ataaatgatt ctgatcctac tggaacgata ataatttcag aagatgacat
      661 gaatgaaatc gaaaagcaat taaagataaa gttcttttct aattgaattg taatattaca
      721 tctgatataa ttgatttaca aattcccggt aaccattccg gcttcattct cttgaagaat
      781 gacatattct cagcgtttta gctgaaggtc agatgatacg tcatctggcc tcttttttta
      841 tataaatgcc ctttaagtga aatatacaac aatctgcaat caatattaga catcacaaaa
      901 cacacaataa attacacaat gtgttataag ccacatgtca ctattatcgc agctagctgc
      961 gtcactcaaa acgaatacgg aacgcgttgg ttatttcaat cataatatta ctctgcaaat
     1021 acacagccct gtaaccgagt aaatggatag agatttgaac aaatgaaaac aatcaactaa
     1081 tggaaaggat aaaatattat gcacaacaat aatgaattca acaaaaaact gaaagatttt
     1141 ataggtagcg ataaacggat ggctttagta aagggttatg ttaacgagta taaattaaca
     1201 actgttttaa gagctttaaa taattccgat tacacaaaag ggacgataca cactcgttca
     1261 ataggcaacc taaaacaaat agtagataat cgaattattc ctaaaaatat aactcaaaac
     1321 acaatcttca aattgagtaa tttaacctta gaggttaacc tctatgaaag aaacacaatt
     1381 tatcacggag actttgctat ttactatcct gtagaatctg ctctaataat agaaacagat
     1441 actcctaggc taattaatca tataaataac aacccagtcc aaaaaatatt tataataaca
     1501 acaaatgact ggagctttaa tacacttgaa ttagaaaaaa tagtagacga aactataatt
     1561 tatgatttaa aacaagaaga tcctaaacaa tatgaaattt tatataaaaa taaacacgga
     1621 aaattgcctt attgatgaat taaaacgcaa acttagaaca ccttacccct tttccgtcct
     1681 tattgtaatt ttccaactac tcaaattaaa aaccacccgt gcaaacaggt ggttttatta
     1741 tatataatta ttcccactca attgtgcttg gtggttttga tgtaatgtca tagactacgc
     1801 ggttgacgtg atctacttcg tttacgatac gactagaaat cttttgtaag acttcccaat
     1861 cgatgcgtgc gaagtcactt gtcataccgt cgatagatgt tactgcacga atacctactg
     1921 tgtgatcata cgtacggtag tctcccataa cacctactga ttgaatgttt ggtaacactg
     1981 tgaagtattg ccaaatttct ctttcaagac cttcttctct aatcacttgg cgtaaaatcg
     2041 cgtctgattc tctaacgatt tctagtttat cttcagtaat ttctccaagt acacgaatac
     2101 caagaccagg tcctgggaat ggttgtctcc atactaaatg ttctggaata cctaactcaa
     2161 tacctaattt acgtacttca tctttaaaca atgtattgat tggttcgatt aattcgaatt
     2221 ccatgtcttc tggtaatcca ccaacattgt ggtgtgattt gattgtttgt gctgttttag
     2281 tacctgattc gatgacgtct gtatatagtg ttccttgcgc aaggaagtct acacctttca
     2341 gttttgatgc ttcatcatca aatacgtata caaattcatt accaatgatt ttacgttttt
     2401 gttcaggatc tgaaacacct tttaatttat tcataaagcg atctttcgca ttaacacgaa
     2461 taatattcat gttgaaacct tcaccgaatt gctccataac catgtcgcct tcacctttac
     2521 gaagtaagcc atggtctaca aagatacatg ttagttgatc acctattgct ttatgcaata
     2581 gtacagctac aacagatgaa tctacgccgc cactcatcgc acataataca cgacggtctc
     2641 ctacgcgttg acgaatcttt tcaatttcga tttcgataaa gttttccatt gtccattgac
     2701 ctctacaatc acaaacacga cggacaaaat tatttaataa atcattacca tattctgtat
     2761 gacgtacttc tggatggaat tgaacaccat aaatgcgacg tttcttatct tcgattgctg
     2821 catagtctgt gcttgggcta tcagcgataa cttcaaagcc ttctggaatt tcaataactt
     2881 tatcagaatg actcatccaa acagtttgtt ctgctggtaa gccagcgaat aactcatctg
     2941 acttcgcatt aatgattgct ttaccgtatt cacgttcatt ggcacgttca actttaccac
     3001 ctaataattt agtagttaat tgcatgccgt aacaaatacc aagtactgga attcctaaat
     3061 tatatatttc cggatcaatt gtaaatgaac cttcttcata aactgaattt ggaccacctg
     3121 ataagataat accttttgga ttcattttct taatttcttc aattgaaatt tcatgatcgt
     3181 gtaattcact ataaacgccc atttcacgaa ttcggcgtgt aattaattgg ttgtattggc
     3241 taccaaagtc taagacaagg attaactctt gttcttttgc catttccata tttgtcgttc
     3301 tcctttatct taattagaat gagtagttcg gtgattcttt cgtaatttga atattatgtg
     3361 gatggctttc tgctaaacca gcaggaccca tacgtgtaaa ttgtgcttct tcgcgtaatt
     3421 ctcttaaatc gtgtgaacca gtataaccca taccagcacg cacaccgccc attaattggt
     3481 aaattgtatc ttgtaacgca cctttataag ccgtacgtcc ttcgatacct tcaggaacaa
     3541 atttcttagg cgctttgtcc tcttggaagt aacggtcgtt tgaacctttt tccatcgcac
     3601 ctaaagagcc cataccacgg tatactttat attgtctacc ttggaaaatt tctgttgcgc
     3661 ctgggctttc ttcagtacct gctaataagc tacctaacat aaccgcatgt ccaccagcag
     3721 ctaatgcttt aatgatatct cctgagaatt taataccacc atcagcaatg atagctttac
     3781 catgtttgcg tgcttcagtt gcacaatcat aaattgctgt aatttgtggt acaccaacac
     3841 ctgctacaac acgcgtcgta caaattgaac ctgggccaat accaacttta acaatatctg
     3901 cacccgcttc aaataaatct tttgttgctt ctgcagttgc tacgttacct gctactaatg
     3961 tgatttctgg gtaagtcttc ttaatatgtt tcacttgatc gataacacct ttagagtgac
     4021 catgtgctgt atcgataact aagacatcca cacctgcttc gactaatttt tgagcacgaa
     4081 tatcagtatc ttttgaaata ccaattgctg cggctacaag tagacgacca tgttcatctt
     4141 ttgctgcatt agggaattcg ataacttttt caatatcttt aatagtaata agaccttcta
     4201 gacgtccgtc tttaactaat ggtaactttt caatcttatg tttttggaga attttttctg
     4261 cttcttcaag tgttgtattc actggagctg taattaaatt ttcttgcgtc attacatcta
     4321 caattttaat cgagaagtct tcaataaaac gtaagtcacg gtttgttaaa atacctacta
     4381 agttgcgatc ttctttatta tcaacaattg gtacacctga aatacggtat ttacccatta
     4441 atgcttctgc ttcataaacg ctttcttctg gcgttaagaa aaatgggttt gaaatgacac
     4501 cattttctga gcgttttact ttttgaactt cgtccgcttg ttcttcaacg cccatatttt
     4561 tatgaataac acctaaacca ccttgacgag ccatagcaat cgccatttta gattcagtta
     4621 cagtatccat accagcagaa ataactggaa tatttaattt aactttgtct gataattgta
     4681 cgcttaaatc aacgtctttc ggtaaaatat cagattgtgc tggaattaat aacacatcat
     4741 caaacgttaa tgattctttt gcaaatttac tttcccacat taaaaacagc ctccattttt
     4801 caaattaatt agttatatta tttcacattt tcttccgttt gtttatactt tattccatta
     4861 aaaaagaaat taagaataat tgcagagatt gcgcctaaaa caattccgtt ttgagtcaac
     4921 catgcaaatt gttcacctaa acctttaaat gcttgtggta cagcgcttat accagtacct
     4981 aatcctactg atacagcgat aattaataaa ttgttttgat ttttaaaatc gatatgtcct
     5041 aatatactaa caccatatgc cattaccatg ccaaacatag ctatcatcgc accgcctaac
     5101 acaggtagcg gtatgatatt tgctaatgcg ccaagcttag gtatacaacc acatataagt
     5161 aataacacga ccatgccgta tataacattg tttttcttag cgccggataa agaaacaagt
     5221 cctacatttt gcgaataggc tgtatacgga aatgaattga atatagaacc taacactatc
     5281 gctagacctt ccgcagtata acctttacga aaatcttttc tttctaactt cttaccggta
     5341 atttcactta acgcatgata gacacctgtc gactcaatta aactaacgat agctacaata
     5401 aagaacacta acgtcgatgt cacatcaaag ctaaatccag agaatctaaa cggcactggg
     5461 atgcctaacc aaccggcatg attgacttga ttaatatcga ccatcccaag taagccagca
     5521 cctatcgttc ctaaaacgag tccaattaat atggcaatac tcttaataaa tccagttgtg
     5581 aatctttgta aaagaagaat aatgattaat gtcattaaac ctaacaaaat gttcttaaca
     5641 tctccatagt cctttgcacc ttgacctcca gctaagtaat tcattgctac tggcattaaa
     5701 ttgataccaa tgatagtaac aacactaccc gttactactg gtgggaagaa ttttacaaga
     5761 tgtgaaaaga aaggcgcgat gataataact aatatccctg ataaaaatag cgaaccataa
     5821 agtacatcta ttcctttcgt ttgaccaatt aaaatcatgg gcgcaacagc cgtgaatgta
     5881 catccaagaa cgattggtaa tcctgttcct gttactttat tggcttgtaa aaatgtggca
     5941 accccacaca taaatatatc tactgtaact aagtaagcga tttgttcagg tgtaaacttc
     6001 aaacttgtac caacaatgat tggaactaag atagcacctg cgtacatagc taaaagatgt
     6061 tgaacactta ggattaaatt tttcattatt cttctcccac caatgtcact ttgtttcctt
     6121 ctagtgaagc aaccttgcag agagaagaaa ctgttaaacc tgcttcttct aaacgttgat
     6181 gcccattttg gaaactcttt tcaacaacaa taccaatacc agctgtctta gcattcgctt
     6241 gctgtgcgat atcgtataat cctaatgaag catcaccatt tgctaaaaag tcatcgatga
     6301 taagtacagt atcttcttct gataaaaact cttttgaaac aatgaccgta cttgttttat
     6361 ttttagtaaa tgaatgaata gatgtttcat aataaccatc cgtcaaagtg ctaggttttg
     6421 cttttttcgc aaataaacat ggcacatcaa aatgcagtgc agccatgatt gcaggtgcga
     6481 taccggaagc ttcaatggtt aagattttag taatcccttt atctttaaat tgctcgtaaa
     6541 aagtgcgacc aacttcattc attaactttg catcaatttg atgatttaaa aatccatcga
     6601 cttttaaaat cttctcatca atgacaacgc cgtcttcctt tactttttgt cctagtaact
     6661 ccactcttaa aacctcctca gatttgtgtg aaacattttg ctcttaaatt ggtgctagat
     6721 acaaaaaaat ccccaaacta aataatagtt tcagggttta tgagtgaacg aacatgcata
     6781 acgaatttgt catgcaatca atgtaaaaga agtttcatca aataaactgt gaccatcata
     6841 taaaatgata taaatcaccc accatggtta caatttaatg gctgaagcta ctcctagtat
     6901 tgtgttgtta ctcatagtca tgtcgttcaa ggcaacatgg tagaaacttc taaagccata
     6961 ttctttagat tatatgagtt tatgtaaatt atttaacgat aatagcaaat tttcggcatt
     7021 ttttcaataa ctgcttaggt aatcttttaa tagttttata aatgtacaga gacgtttaat
     7081 gcgaatgaag acttataact taataatgta agggttttac tttataataa gaagggtaaa
     7141 agattaaata aacaaatttt taaaggggcg acaactatgg cagatattac tgtagtaaat
     7201 gacactggcg aattatacaa cgtaatcaat caaaagaaat ctgaaggtta tttagaatca
     7261 gagctaacaa ttattagtaa aagtaaatta cacctaaatg acttacacga ctctgaaatc
     7321 tcccttatct caacaagtgg aacatttagt gatagaatga ctaaactgtt aacaggagaa
     7381 gatggtgaac atgcagtttt atcacgctac aacttagcgc ctgatgaatt agaaaaatat
     7441 aaacaactta ttttagacga taaaatgtta gttgtagcgg ttcgtgacaa atcatcacat
     7501 caagaagtgc atgaaaataa ttcagcttat gaagagattg atattacaca ttttgctgaa
     7561 gcatctaaag gtcccaaagc ttagttatca cactgaattc aaaatgagat tactgtactg
     7621 tctaatttaa gatagtacag ttttttatac catttaaaag tttatgaaca gtcttttaca
     7681 gcaggcgatt atgctataat attatttaaa ttggggaggt gttcttatgg aatttaaaac
     7741 cattcaatct gtcaaagatc cacattacaa cgaagcactt aaactttacg atactaagtt
     7801 aaatttatct ttaactgaag ataaacgtat catcaaacgc tcattagaaa acaacaaaac
     7861 cgaaaacgat tatgtcttta tcgtaggctt agaaaacgat gttgttgtca gtctagcaac
     7921 tgcacattat gaagccacaa ccaattcagc atttttaatt tatttattcg caaaagacct
     7981 acccaaccat gatgagttaa tgacgcaaac attacatcaa attgaacatg aactaaactt
     8041 attatcaaac cttgttcatt ctagaagtat taacttttta atgatggaag tccctaaaga
     8101 acctgcagat ttaacacaag ataacggcct cattattgaa caccgtagac agtttttata
     8161 cgaacatcaa tttgaaagac aaactgaaat tgattacatt catccaacta atgatggtca
     8221 cactaaaaaa gtggacttat ttttaaagaa aaatattaaa ctgacaaagg atatctacgg
     8281 tacaagtgtt aaatcaaatt atattttgaa gtatgtattt gccaatggta tcgcacgtga
     8341 aattatttat gctttattag aagaaatgga tttacgtaaa ccattttaaa caaacttgaa
     8401 atcactaaca ataacgttta aactatgtat gaagcaagac tgacgtttca ctgcccgtag
     8461 acaaacgtca gtatacataa agtatatgca gaaaggactt taaagtatgt taactaaaga
     8521 atttgcacaa cgcgtagaac taagtgagaa gcaagtacgt aaaattgttc aacatttaga
     8581 agaacgtggt taccaactaa gcaagactga atatcgtggt cgtgaagcaa ctgatttcaa
     8641 agaagaagat atcgagcttt tcaaagacat tgccgacaaa gtaaaacaaa caaatagtta
     8701 tgatctagcg tttgatgaat tagaaaaaga aaaagacttc ctgcaagtca ttgtcaaaaa
     8761 cgatgacaaa aacttaccta ctaatcaaaa tgtcgctcaa ctagtagaag atttacgcct
     8821 agaaatccag aaaatgcgcg aagaacgtca cctacttggt caaatgatga atcaagtaca
     8881 tcagcaacaa caagaattaa aagaacttca aaatcaactt acatctaaaa tcgattcaaa
     8941 tagcgaatcc ttaaaagcca tccaaacatc acaagaggct atccaagaag cgcaagcctc
     9001 tcaagcaaaa gcattagctg aatcaacaaa taaagttgaa aagaatgctg ttactgaaga
     9061 taaagctgac agtaaagatt cgaaggttgc tggagtgaat acatcaacag acgctaagac
     9121 ggatactaaa gcagaaaatg caggcgatgg cactgctact aaagtagata aagaggatca
     9181 aatttctgca actgaagcaa ttgaaaaagc cagcgttgag cagtctaaaa atgacaatgc
     9241 agctgagact tcaaataaag aagctacagt agatgcagaa gcgcaacatg atgctgaaca
     9301 acaagtagca gaagcccatg cagaagcttc taaacaagct acttcgaacg attcattgga
     9361 agcgaaagca gaaaatgaca gcacagcttc acagtcagaa atgtcagaac caaaacctca
     9421 agaagagaaa aaaggtttct tcgcacgttt attcaacctg taaacttgtg atttgaacac
     9481 aaatttaata ctcaattcat atatagcttt gatttaaaaa ctgattttta taaacctata
     9541 gaactgtaat ttactttcaa atttaatact caattcataa aacctgaaac aagctgatat
     9601 actccattca atatctactt tgaagagagc atatcaaact aatttgtttc aggtttttta
     9661 attattcatt atgattaata ttgaattttg aataaatttt tatagtatgt tacttttaaa
     9721 tgggatggct tctgtgtact tctaaataga ttcatcttaa taagtccatt caatttaaat
     9781 gtttggctgt gttagtaact gacgaatcgc acaagtgcac actcatattc acatttacta
     9841 caaacgcctt tttgctcaaa caaaaatatt tgtttacggt agcttttgaa ctaccacctg
     9901 cacacacgaa cactcaaaag atgctaattt tgattcgaca gaatttttta caacgtgttt
     9961 gttcgaaacg tcttttcaag gttgatcatt tattatcttt tcattcaata aatatgaggt
    10021 tagcaatttt tatcaaaccg ccatagccac atctcgattc aaggataaat ttgttttctc
    10081 atatcttatc tctcatgaca gtgcacgata tatatctttt ccaaatattc atcacttttt
    10141 tcctgttaaa atcatttcta ttattaatgc tttaatgaca acttatttaa tttattgcat
    10201 ttaatgccct gcttcatttt caaatattaa ctttaatcga ctagctcatc acactattta
    10261 ccctttccac ctatcactac tgaattcaaa acgatttgtt tacaaatccc cttatgccaa
    10321 cggtcgaatt tcatcatgtt tacatttaat tgcaaatgcc ctcttgaaat attttaaaat
    10381 taatattatt cttagcatac tagtcggaat tatattcaaa aatttgagga gtgacgttca
    10441 tgaatgcttt tcttacactg attaatatta ttgtattggt gattttcatc gtaatattgc
    10501 acatgatggc tcgcaaacat atatcttttg cgaaacgtgt gtttacagct ttagggattg
    10561 gtatagtatt cggtgtgtta ttgcatctta tttatggcac acattcgaat gttattacat
    10621 caacaagtga ctggttcaac attgtcggac aaggttacgt tgcattgttg caaatgattg
    10681 ttatgccttt aattttcatt tctattgtag ctgcattcac taaaattcaa atcggagaaa
    10741 agtttgctaa aattggtagt ctcattttta tctttttaat tggcactgtc accattgctg
    10801 cgattgttgg tgttgtgtat gccttggtgt ttggtttaga tgcatcaaca attaaccttg
    10861 gtaacgctga acaagcacgt ggaagcgaaa ttgctaaaca agcgaaagat ttaacagcac
    10921 atacgttacc acaacaaatt ttggaattat tgcctaaaaa tccattctta gatttcactg
    10981 gacaacgtgc aacttccacg attgctgtcg tgatatttgc ttcgtttata ggatttgctt
    11041 atttacgcgt agcacgcaaa caacctgatc atggtgaatt gcttaagcgt gcgattgacg
    11101 ctatctattc actagtaatg gcgattgtga cgttcgtctt acgtttaacg ccatacggtg
    11161 tattagcaat tatggccaac acattatcga caagtgactt tggcgccatt tggactttag
    11221 gaaaattctt aatcgcatcg tacgctgcat taattacgat gtatatcatt cacttaatta
    11281 ttttaagcct gttaggtatt agcccaattc gctatgttaa aaagactttg gaagtattaa
    11341 tctttgcatt cacatcacgc tcaagcgcgg gtgccttacc attgaatgta caaacacaaa
    11401 caagacgtct aggcgtgcca gaaggtattg ccaatttcgc tgcgacattt ggattgtcga
    11461 ttggacaaaa tggttgtgca ggcatttacc ctgccatgct tgcaattatg gtcgcacccg
    11521 tcgctaatgt agaaattgat ttacaattta ttgtaacgtt aatcgctgtc gtgattatta
    11581 gttcattcgg cgttgctggc gtcggtggcg gggctacatt tgcatccata ttggtgttgt
    11641 caacgttaaa cttgccagtc gctttagccg gcgtgctcat ctccgttgaa ccattgattg
    11701 acatgggtag aacagcatta aacgtgaacg attcaatgct agctggtaca ggtaccgcca
    11761 agttaactaa acattgggat aaagatactt tcgattcaaa tgataatgct gcattaactt
    11821 cacactaatt cattgcaaga aatacccgcc ccctctagct atacttatca ttttaagcta
    11881 gaggggggcg ggttattttg gtatcttgct atcgctgtat taagcctgat ttttgcaatt
    11941 gttctaacat atctaatctt gctttgtttc ctaggaacat ctcaatttgc tgcgaccatg
    12001 tttctttgcg attcccgttt gtacgttgat catagtactc gctgattgtc tggtcgtaat
    12061 ctgccatttg tgcatactgt gtttccttat cagcatgata cttattatga tggaagacat
    12121 ggtcaaatgg taagcgtggc ttggctgcac cattttcgtc atctgcgggt tcccctactg
    12181 ccataccaaa taccgggaag acatagtcag gtaagtctaa aatttctcga acgcgttcaa
    12241 catcatttct taatgatcct aaaaagacaa tgccataccc catatcttca gcagttaccg
    12301 caatattttc ggcaactaat gctgcatcga ttgcacctac tagcaaacct tccgttgaac
    12361 catatgcatt ttccatatca gtttcagcat gttgatcaac taaatgatga cgataataat
    12421 caataacaaa gacgaataaa tagccatttt ctacaacata aggttgtcca gaaacttctc
    12481 gtaaattttc tttaatcttc tcatcgtcga taccaataat tgagtatgct tgcaggaaac
    12541 ttgacgtcga agcgctttgt ccagcttcta ccaatttctt aacaacgtct tcacttaaag
    12601 gtttattctt aaattttcta acagaatgat gctttttcac aagattatat acatgttctg
    12661 acacgttacc cactcctcga tactttacaa ttaatttaaa atcttcatat tgcaattgta
    12721 cacgtccaaa cttttttata gcaattattt gcttataagt taataattga actttttaaa
    12781 atattgtctc atactttaaa aatccagttt ctcactcatt accttcat
//
LOCUS       DAKTIO010000068        12823 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.068, whole genome shotgun sequence.
ACCESSION   DAKTIO010000068 DAKTIO010000000
VERSION     DAKTIO010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12823
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.068"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            135..1007
                     /gene="dprA"
                     /locus_tag="O0293_001434"
     CDS             135..1007
                     /gene="dprA"
                     /locus_tag="O0293_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCY0280323.1"
                     /translation="MIKLFLLKLYWAHFSTKQIHQFLMAYPNVIKEEGRKKDSYLCEW
                     VNREENVHLLRKYYAFIKLDHNDIIKELQKLKVSYITYMDSEYPVLLKEIYQFPLLLF
                     YKGNIKLINNMHHLAVVGARDSTSYTQQSLEFLLSNDKSKYLTIVSGLAQGADAMAHQ
                     IALKYNLPTIAVLAFGHQTHYPKSTLALRNKIEEKGLVISEYPPHTPIAKYRFPERNR
                     IISGLSKGVLITEAKEQSGSHITIDFALEQNRNVYVLPGSMFNPMTKGNLLRIQEGAK
                     VVLNANDIFEDYYI"
     gene            1181..3256
                     /gene="topA"
                     /locus_tag="O0293_001435"
     CDS             1181..3256
                     /gene="topA"
                     /locus_tag="O0293_001435"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCY0280324.1"
                     /translation="MTLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQM
                     GVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILEL
                     EDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKV
                     KKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPF
                     KLKTKKDVEKITAALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKT
                     MMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKVEAKQYITDKYGESYTSKRKA
                     SGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVS
                     LDITQGDIKFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPA
                     QHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEI
                     VHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEME
                     KIEIKDEPAGEDCEICGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCND
                     GDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSN
                     CDYKEAAQK"
     gene            3412..4719
                     /gene="trmFO"
                     /locus_tag="O0293_001436"
     CDS             3412..4719
                     /gene="trmFO"
                     /locus_tag="O0293_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HCY0280325.1"
                     /translation="MTQTVNVIGAGLAGSEAAYQLAERGIKVNLIEMRPVKQTPAHHT
                     DKFAELVCSNSLRGNALTNGVGVLKEEMRRLNSIIIEAADKARVPAGGALAVDRHDFS
                     GYITETLKNHENITVINEEINAIPDGYTIIATGPLTTETLAQEIVDITGKDQLYFYDA
                     AAPIIEKESIDMDKVYLKSRYDKGEAAYLNCPMTEDEFNRFYDAVLEAEVAPVNSFEK
                     EKYFEGCMPFEVMAERGRKTLLFGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNI
                     VGFQTHLKWGAQKEVIKLIPGLENVDIVRYGVMHRNTFINSPDVLNEKYELISQPNIQ
                     FAGQMTGVEGYVESAASGLVAGINLAHKILGKGEVVFPRETMIGSMAYYISHAKNNKN
                     FQPMNANFGLLPSLETRIKDKKERYEAQANRALDYLENFKKTL"
     gene            5136..6032
                     /gene="xerC"
                     /locus_tag="O0293_001437"
     CDS             5136..6032
                     /gene="xerC"
                     /locus_tag="O0293_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCY0280326.1"
                     /translation="MNHIQDAFLNTLKVERNFSEHTLKSYQDDLIQFNQFLEQEHLEL
                     NTFEYRDARNYLSYLYSNHLKRTSVSRKISTLRTFYEYWMTLDENIINPFVQLVHPKK
                     EKYLPQFFYEEEMEALFKTVEEDTSKNLRDRVILELLYATGIRVSELVNIKKQDIDFY
                     ANGVTVLGKGSKERFVPFGAYCRQSIENYLEHFKPIQSCNHDFLIVNMKGEAITERGV
                     RYVLNDIVKRTAGVSEIHPHKLRHTFATHLLNQGADLRTVQSLLGHVNLSTTGKYTHV
                     SNQQLRKVYLNAHPRAKKENET"
     gene            6029..6574
                     /gene="hslV"
                     /locus_tag="O0293_001438"
     CDS             6029..6574
                     /gene="hslV"
                     /locus_tag="O0293_001438"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCY0280327.1"
                     /translation="MSNTTLHATTIYAVRHNGKAAMAGDGQVTLGQQVIMKQTARKVR
                     RLYEGKVLAGFAGSVADAFTLFEKFETKLQQFSGNLERAAVELAQEWRGDKQLRQLEA
                     MLIVMDKDAILVVSGTGEVIAPDDDLIAIGSGGNYALSAGRALKRHASHLSAEEMAYE
                     SLKVAADICVFTNDNIVVETL"
     gene            6640..8043
                     /gene="hslU"
                     /locus_tag="O0293_001439"
     CDS             6640..8043
                     /gene="hslU"
                     /locus_tag="O0293_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HCY0280328.1"
                     /translation="MDTAGIRLTPKEIVSKLNEYIVGQNDAKRKVAIALRNRYRRSLL
                     DEESKQEISPKNILMIGPTGVGKTEIARRMAKVVGAPFIKVEATKFTEVGYVGRDVES
                     MVRDLVDVSVRLVKAQKKSLVQDEATAKANEKLVKLLVPSMKKKASQTNNPLESLFGG
                     AIPNFGQNNEDEEEPPTEEIKTKRSEIKRQLEEGKLEKEKVRIKVEQDPGALGMLGTN
                     QNQQMQEMMNQLMPKKKVEREVAVETARKILADSYADELIDQESANQEALELAEQMGI
                     IFIDEIDKVATNNHNSGQDVSRQGVQRDILPILEGSVIQTKYGTVNTEHMLFIGAGAF
                     HVSKPSDLIPELQGRFPIRVELDSLSVEDFVRILTEPKLSLIKQYEALLQTEEVTVNF
                     TDEAITRLAEIAYQVNQDTDNIGARRLHTILEKMLEDLSFEAPSMPNAVVDITPQYVD
                     DKLKSISTNKDLSAFIL"
     gene            8068..8841
                     /gene="codY"
                     /locus_tag="O0293_001440"
     CDS             8068..8841
                     /gene="codY"
                     /locus_tag="O0293_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HCY0280329.1"
                     /translation="MSLLSKTRELNTLLQKHKGIAVDFKDVAQTISSVTVTNVFIVSR
                     RGKILGSSLNELLKSQRIIQMLEERHIPSEYTERLMEVKQTESNIDIDNVLTVFPPEN
                     RELFIDSRTTIFPILGGGERLGTLVLGRVHDDFNENDLVLGEYAATVIGMEILREKHS
                     EVEKEARDKAAITMAINSLSYSEKEAIEHIFEELGGTEGLLIASKVADRVGITRSVIV
                     NALRKLESAGVIESRSLGMKGTFIKVKKEKFLDELEKSK"
     gene            8892..8984
                     /locus_tag="O0293_001441"
     CDS             8892..8984
                     /locus_tag="O0293_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280330.1"
                     /translation="MAVVNEMTLMIMLKSFLYFCLIFNQIDISH"
     gene            9183..9950
                     /gene="rpsB"
                     /locus_tag="O0293_001442"
     CDS             9183..9950
                     /gene="rpsB"
                     /locus_tag="O0293_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCY0280331.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKKVDEAYNFLKQVSEDGGQVLFVGTKKQAQESVKSEAERAGQFYINQRWLGGLLT
                     NYKTISKRIKRISEIEKMEEDGLFEVLPKKEVVELKKEYDRLIKFLGGIRDMKSMPQA
                     LFVVDPRKERNAIAEARKLNIPIVGIVDTNCDPDEIDYVIPANDDAIRAVKLLTAKMA
                     DAILEGQQGVSNEEVAAEQNIDLDEKEKSEETEATEE"
     gene            9984..10097
                     /locus_tag="O0293_001443"
     CDS             9984..10097
                     /locus_tag="O0293_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280332.1"
                     /translation="MGDKILMLITFFKKKIEANYKYSIRVLAILPIIMLKS"
     gene            10132..11013
                     /locus_tag="O0293_001444"
     CDS             10132..11013
                     /locus_tag="O0293_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCY0280333.1"
                     /translation="MATISAKLVKELREKTGAGMMDCKKALTETDGDIDKAIDYLREK
                     GIAKAAKKADRIAAEGLVHVETKGNDAVIVEINSETDFVARNEGFQELVKEIANQVLD
                     TKAETVEALMETTLPNGKSVDERIKEAISTIGEKLSVRRFAIRTKTDNDAFGAYLHMG
                     GRIGVLTVVEGSTDEEAARDVAMHIAAINPKYVSSEQVSEEEINHEREVLKQQALNEG
                     KPENIVEKMVEGRLRKYLQEICAVDQDFVKNPDVTVEAFLKTKGGKLVDFVRYEVGEG
                     MEKREENFADEVKGQMK"
     gene            11150..11872
                     /locus_tag="O0293_001445"
     CDS             11150..11872
                     /locus_tag="O0293_001445"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCY0280334.1"
                     /translation="MAQISKYKRVVLKLSGEALAGEKGFGINPVIIKSVAEQVAEVAK
                     MDCEIAVIVGGGNIWRGKTGSDLGMDRGTADYMGMLATVMNALALQDSLEQLDCDTRV
                     LTSIEMKQVAEPYIRRRAIRHLEKKRVVIFAAGIGNPYFSTDTTAALRAAEVEADVIL
                     MGKNNVDGVYSADPKVNKDAVKYEHLTHIQMLQEGLQVMDSTASSFCMDNNIPLTVFS
                     IMEEGNIKRAVMGEKIGTLITK"
     gene            11891..12445
                     /gene="frr"
                     /locus_tag="O0293_001446"
     CDS             11891..12445
                     /gene="frr"
                     /locus_tag="O0293_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCY0280335.1"
                     /translation="MSDIINETKSRMQKSIESLSRELANISAGRANSNLLNGVTVDYY
                     GAPTPVQQLASINVPEARLLVISPYDKTSVADIEKAIIAANLGVNPTSDGEVIRIAVP
                     ALTEERRKERVKDVKKIGEEAKVSVRNIRRDMNDQLKKDEKNGDITEDELRSGTEDVQ
                     KATDNSIKEIDQMIADKEKDIMSV"
BASE COUNT         4863 a         1812 c         2465 g         3683 t
ORIGIN      
        1 ccaacatact tttcactttt taagtcagta tttttactcc ttaaaataac attacatcga
       61 caatgttttt gtattagaaa tgtaaaacaa aaatagaata tgattttgta tgattacaaa
      121 aaggagttga tttattgatt aaactatttt tgcttaagtt atactgggca cacttttcga
      181 ctaaacaaat tcatcaattt ttaatggcat atcctaatgt aattaaagag gagggaagaa
      241 aaaaagatag ttatttatgt gaatgggtga atagggaaga aaatgttcat ttattacgta
      301 aatactatgc ttttataaaa cttgatcata acgatattat taaagaactg cagaaattaa
      361 aagtaagtta cattacatat atggattctg aatacccagt gctattaaaa gaaatatatc
      421 aatttccatt acttcttttc tataaaggga acatcaaatt aataaataat atgcatcatt
      481 tggcagtagt aggtgcaaga gattctacaa gttataccca acagtcttta gaatttttat
      541 tatcaaatga taaaagcaaa tatttaacaa ttgtttccgg ccttgctcaa ggagctgatg
      601 caatggcaca tcaaatagct ttaaaataca atctccctac aattgcagtt ttagcctttg
      661 gccatcaaac acattatccc aaaagtacat tagcattaag aaataaaata gaagaaaaag
      721 gtttagttat atctgaatat ccaccacata caccaatcgc taaatataga tttcctgagc
      781 gcaatagaat tatcagcggt ttgtcaaaag gggttttaat tactgaggct aaggaacaaa
      841 gtggcagtca catcacgata gattttgcat tagagcaaaa tagaaatgtt tatgttttac
      901 ctggatctat gtttaatcct atgacaaaag gtaatttatt acgtatccaa gaaggtgcta
      961 aggtagtatt aaacgctaat gatatatttg aagactacta tatttaaaac taaaaataac
     1021 gagttgcttc atatttcttt tgtttccctt tgtaatttat tttttaataa aatctataaa
     1081 aaatagacag ggaaaatgat ttgtttagat ataaaacgtt gacaaaagca aaattaagcg
     1141 tttatcattt atctttagta attagattag cgagggggaa atgacattgg cagataattt
     1201 agtcattgtt gaatcgcctg caaaagcaaa aaccattgaa aagtatttag gtaagaaata
     1261 taaagttata gcttcaatgg gacacgtcag agacttacca agaagtcaaa tgggtgtcga
     1321 cactgaagat aattacgaac caaaatatat aacaatacgc ggaaaaggtc ctgttgtaaa
     1381 agaattgaaa aaacatgcaa aaaaagcgaa aaacgtcttt ctcgcaagtg accccgaccg
     1441 tgaaggtgaa gcaattgctt ggcatttatc aaaaatttta gagcttgaag attctaaaga
     1501 aaatcgcgtt gttttcaacg aaataactaa agacgctgtt aaagaaagtt ttaaaaatcc
     1561 tagagaaatt gaaatgaact tagtcgatgc acaacaagcg cgtcgaatat tagatagatt
     1621 agttggctat aacatctcgc cagttctttg gaaaaaagta aaaaaagggt tgtcagcggg
     1681 tcgagttcaa tctgttgcac ttcgtttagt cattgaccgt gaaaatgaaa ttcgaaactt
     1741 taaaccagaa gaatattgga ctattgaagg agaatttaga tacaaaaaat caaaattcaa
     1801 tgctaaattc cttcattata aaaataaacc ttttaaacta aaaacgaaaa aagatgttga
     1861 gaaaattaca gctgcattag atggagatca attcgaaatt acaaacgtga ctaaaaaaga
     1921 aaaaacgcgt aatccagcaa acccatttac aacttctaca ttacaacaag aggcggcacg
     1981 taaattaaac tttaaagcaa gaaaaacaat gatggtcgca caacaattat atgaaggtat
     2041 agatttgaaa aaacaaggta cgattggttt aataacatat atgagaaccg attctacacg
     2101 tatttcagat actgccaaag ttgaagcaaa acagtatata actgataaat acggtgaatc
     2161 ttacacttct aaacgtaaag catcagggaa acaaggtgac caagatgccc atgaggctat
     2221 tagaccttca agtactatgc gtacgccaga tgatatgaag tcatttttga cgaaagacca
     2281 ataccgatta tacaaattaa tttgggaacg atttgttgct agtcaaatgg ctccagcaat
     2341 acttgataca gtctcattag acataacaca aggtgacatt aaatttagag cgaatggtca
     2401 aacaatcaag tttaaaggat ttatgacact ttatgtagaa actaaagatg atagtgatag
     2461 cgaaaaggaa aataaactgc ctaaattaga gcaaggtgat aaagtcacag caactcaaat
     2521 tgaaccagct caacactata cacaaccacc tccaagatat actgaggcga gattagtaaa
     2581 aacactagaa gaattgaaaa ttgggcgacc atcaacttat gcaccgacaa tagatacgat
     2641 tcaaaagcgt aactatgtca aattagaaag taagcgtttt gttcctactg agttgggaga
     2701 aatagttcat gaacaagtga aagaatactt cccagagatt attgatgtgg aattcacagt
     2761 gaatatggaa acgttacttg ataagattgc agaaggcgac attacatgga ggaaagtaat
     2821 cgacggtttc tttagtagct ttaaacaaga tgttgaacgt gctgaagaag agatggaaaa
     2881 gattgaaatc aaagatgagc cagccggtga agactgtgaa atttgtggtt ctcctatggt
     2941 tataaaaatg ggacgctatg gtaagttcat ggcttgctca aacttcccgg attgtcgtaa
     3001 tacaaaagcg atagttaagt ctattggtgt taaatgtcca aaatgtaatg atggtgacgt
     3061 cgtagaaaga aaatctaaaa agaatcgtgt cttttatgga tgttcgaaat atcctgaatg
     3121 cgactttatc tcttgggata agccgattgg aagagattgt ccaaaatgta accaatatct
     3181 tgttgaaaat aaaaaaggca agacaacaca agtaatatgt tcaaattgcg attataaaga
     3241 ggcagcgcag aaataatatt tttatttcct agatacattt taagattgtt aaatagaatc
     3301 attagtgaat cttattttaa agatagtaaa ggattaatct aaataagtgc ggataatata
     3361 aacataacaa cataattaaa agacataaat gacaataaaa ggagtataga aatgactcaa
     3421 actgtaaatg taataggtgc tggtcttgcc ggttcagaag cggcatatca attagctgaa
     3481 agaggaatta aagttaatct aatagagatg agacctgtta aacaaacacc agcgcaccat
     3541 actgataaat ttgcggaact tgtatgttcc aattcattac gcggaaatgc tttaactaat
     3601 ggtgtgggtg ttttaaaaga agaaatgaga agattgaatt ctataattat tgaagcggct
     3661 gataaggcac gagttccagc tggtggtgca ttagcagttg atagacacga tttttcaggt
     3721 tatattactg aaacacttaa aaatcatgaa aatatcacag ttattaatga agaaattaat
     3781 gccattccag atggatacac aattatcgca acaggaccac ttactacaga aacccttgcg
     3841 caagaaatag tggacattac tggtaaagat caactttatt tctatgatgc ggctgctcca
     3901 attattgaaa aagaatctat tgatatggat aaagtttact taaagtcccg ttatgataaa
     3961 ggtgaagctg catatttaaa ctgtcctatg actgaggatg aatttaatcg cttttatgat
     4021 gcagtattag aagctgaagt tgcgcctgta aattcatttg aaaaagaaaa atatttcgag
     4081 ggttgtatgc cttttgaagt aatggcagaa cgcggacgca agacattact atttggacca
     4141 atgaaaccag taggattaga agatccaaag actgggaaac gtccttatgc ggtggttcaa
     4201 ttaagacaag atgacgctgc tggtacactc tacaatattg ttggcttcca aacgcattta
     4261 aaatggggag ctcaaaaaga agtcattaaa ttaattccag gcttagaaaa tgttgatatt
     4321 gttagatatg gtgtgatgca tagaaatacc ttcattaatt caccggacgt attaaacgag
     4381 aaatatgaat tgatttcaca accaaacata cagtttgcgg gacaaatgac tggtgttgaa
     4441 ggttatgtag aaagcgcagc tagcggctta gttgcaggta tcaatcttgc gcataaaata
     4501 ttaggcaagg gtgaggtagt atttccgaga gaaacaatga ttggaagtat ggcttactat
     4561 atttctcatg ctaaaaacaa taagaatttc caacctatga atgctaactt cgggttatta
     4621 ccatctttag aaactagaat taaagataaa aaagaacgct atgaagcaca agctaataga
     4681 gctttggatt acttagaaaa tttcaaaaaa actttataaa atagttagaa agactagata
     4741 tgctattcat tcttaagtca tcaacgagta agtaatgact ttctaaatgg aaaatactta
     4801 tcctagtctt tttaattttg gaattgttac gtatttctga caatttagaa ttcgcattca
     4861 aaaaatatct aaataaataa cacgcaataa gttgattgat gtaacatgta agagaatgtt
     4921 ttaaataaac tttatttaaa aggcaatgaa ataataaatg gcaaggctat taataaagac
     4981 ttttagtaat taatttaaaa aagaggtatt ctaattaaca ggttttccga ttagttacaa
     5041 ttatttaatt ctcaaaagat ttagaattga ttatcaaatt actgtaagcc ctttgctgta
     5101 tatgctacaa ttcttattga tggagggtaa atgtattgaa tcatattcaa gatgcgtttt
     5161 taaatacatt gaaagttgaa cggaattttt cggaacacac attgaaatca tatcaagatg
     5221 acttaattca gtttaatcaa tttttagaac aagaacattt agagttgaat acttttgaat
     5281 acagagatgc tagaaattat ttgagctatt tatattcaaa tcatttgaaa agaacatctg
     5341 tttctcgtaa aatctcaacg ttaagaactt tctatgaata ttggatgacg cttgatgaga
     5401 acattattaa tccatttgtt caattagtac atccgaaaaa agaaaaatat cttccgcaat
     5461 tcttttacga agaagaaatg gaagcgttat tcaaaactgt agaagaggac acttcaaaaa
     5521 atttacggga tcgagttatt cttgaattgt tgtatgctac aggcatccgt gtttcggaat
     5581 tagtaaatat taaaaaacaa gatatagatt tttacgcgaa tggtgttacc gtattaggaa
     5641 aagggagcaa agagcgcttt gtaccgtttg gtgcttattg tagacaaagc atcgaaaatt
     5701 atttagaaca tttcaaacca attcagtcat gcaatcatga ttttcttatt gtaaatatga
     5761 agggtgaagc aatcactgaa cgcggtgtac gatatgtttt aaatgatatt gttaaacgaa
     5821 cagcaggcgt aagtgagatt catccccaca agctcagaca tacatttgca acgcatttat
     5881 tgaatcaagg tgcagaccta agaacagtac aatcgttatt aggtcatgtt aatttgtcaa
     5941 caactggtaa atatacacac gtatctaacc aacaattaag aaaagtgtat ctaaatgcac
     6001 atcctcgagc gaaaaaggag aatgaaacat gagtaataca acattacatg caacaacaat
     6061 ttatgctgta agacataatg ggaaagcagc tatggctgga gatgggcaag taacgcttgg
     6121 tcaacaagtc atcatgaaac aaacggcaag aaaagtgcga cgtttatatg aaggtaaagt
     6181 gttagctggt ttcgcaggta gtgtagcaga tgcgtttacg ttatttgaaa aattcgaaac
     6241 aaaattacaa cagtttagtg gtaacttaga aagagctgct gttgaattgg cacaagaatg
     6301 gcgaggcgat aaacaattac gtcaattaga agctatgcta attgtaatgg ataaagatgc
     6361 tattttagtt gtcagtggaa ctggcgaagt tattgctcca gatgatgacc ttatcgctat
     6421 tggatcagga ggcaactacg cattaagcgc aggacgtgca ttgaaacgcc atgcatcgca
     6481 tttgtctgct gaagaaatgg catatgagag cttgaaagta gcggctgata tttgtgtctt
     6541 taccaacgat aatattgttg tcgaaacact ataataatca gagcacgata aataattacg
     6601 agcaattaat tttagttaaa agacggagga atgaaattaa tggatacagc tggaataaga
     6661 ttaactccaa aagaaatcgt atctaaatta aatgaataca tcgttggaca aaatgatgct
     6721 aaacgtaaag tggcaattgc cctacgtaat cgatacagaa gaagtttatt agatgaggaa
     6781 tcaaagcaag aaatttcacc taaaaatatt ttgatgattg gaccaaccgg cgttggtaaa
     6841 actgaaattg caagaagaat ggccaaagtt gtcggcgcgc catttataaa agtagaagct
     6901 actaaattta ctgaggtagg ttatgtagga cgagatgttg aaagtatggt tagagatctt
     6961 gttgatgttt cagtaagatt agtcaaggcg cagaaaaaat cattggtaca agatgaagca
     7021 acagctaagg ccaatgaaaa acttgttaag ttattagttc caagtatgaa aaagaaagcg
     7081 tctcaaacga ataatccttt agagtcactt ttcggaggtg caattccaaa tttcggacaa
     7141 aataacgaag atgaagaaga accacctact gaggaaatta aaacaaaacg ttctgaaatt
     7201 aagagacagc tagaagaagg caaacttgaa aaagaaaagg taagaattaa agtcgaacaa
     7261 gatcctggtg ctttaggtat gctaggtaca aatcaaaatc agcaaatgca agagatgatg
     7321 aatcaattaa tgcctaaaaa gaaagttgag cgagaagttg ctgttgagac ggcaaggaaa
     7381 atcttagctg atagttatgc ggatgaacta attgatcaag aaagcgctaa ccaagaagcg
     7441 cttgaattag cagaacaaat gggtatcatc tttatagatg aaatcgacaa agttgcgacg
     7501 aataatcata atagtggtca agatgtctca agacaaggtg ttcaaagaga tattttacct
     7561 atacttgaag gtagcgttat tcaaaccaaa tatggtactg tgaatactga acatatgctg
     7621 tttataggtg ctggagcttt ccatgtatct aagccgagtg acttgatacc agaattgcaa
     7681 ggtcgttttc cgattagagt tgaacttgat agtttatcgg tagaagattt tgtaagaatt
     7741 ttgacagaac caaaattgtc attaattaaa caatatgaag cattgcttca aacagaagaa
     7801 gttactgtaa actttaccga tgaagcaatt actcgcttag ctgagattgc ttatcaagta
     7861 aatcaagata cagacaacat tggtgcacgt cgacttcata caattttaga aaagatgcta
     7921 gaagatttat cattcgaagc accaagtatg ccgaatgcag ttgtagatat taccccacaa
     7981 tatgttgatg ataaattaaa atcaatttca acaaataaag atttaagtgc atttattcta
     8041 taaaaatata caaaaggaga aaaattcatg agcttattat ctaaaacgag agagttaaac
     8101 acgttacttc aaaaacacaa aggtattgcg gttgatttta aagatgtagc acaaacgatt
     8161 agtagcgtaa ctgtaacaaa tgtatttatt gtatcgcgtc gaggtaaaat tttaggatcg
     8221 agtctaaatg aattattaaa aagtcaaaga attattcaaa tgttggaaga aagacatatt
     8281 ccaagtgaat atacagaacg attaatggaa gttaaacaaa cagaatcaaa tattgatatc
     8341 gacaatgtat taacagtatt cccacctgaa aacagagaat tattcataga tagtcgtaca
     8401 actatcttcc caattttagg tggaggggaa agattaggta cattagtact tggtcgagta
     8461 catgatgatt ttaatgaaaa tgatttggta ctaggtgaat atgctgctac agttattggt
     8521 atggaaatct tacgtgagaa gcatagtgaa gtagaaaaag aagcgcgcga taaagctgct
     8581 attacaatgg caattaattc attatcttat tctgaaaaag aagcgattga acatatcttt
     8641 gaagaacttg gcggtacgga aggcctatta atcgcatcaa aagttgcaga tagagttggt
     8701 attactagat ctgtaattgt aaatgcacta cgtaaattag aaagtgctgg tgtaattgaa
     8761 tcacgttctt taggaatgaa aggtactttc attaaagtta aaaaagaaaa attcttagat
     8821 gaattagaaa aaagtaaata agttgatgag tctgggacat aattcaatgt cctagtcgtg
     8881 acgatgttat cttggcggta gtcaatgaaa tgactttgat gattatgttg aagtcatttc
     8941 tttatttttg tctaattttc aatcaaattg atatttcaca ttaacttgat agaattacct
     9001 ttaaatttat ggaaaaatgc ttgaaaaaca tttgctttag acacggcatt atgttatatt
     9061 tattttcggc tataaagcca atacacacat atcaaacgtg attgtgtaaa gggtgcaata
     9121 tttatatatt gtctttacaa tagtttgcta tggaggtaat taaccaatag gaggaattta
     9181 taatggcagt aatttcaatg aaacaattac tagaagcggg tgttcacttc ggtcaccaaa
     9241 cacgtcgttg gaacccaaaa atgaaaaaat atatcttcac tgagagaaat ggtatttata
     9301 tcatcgactt acaaaaaaca gtgaaaaaag tagacgaggc atacaacttc ttgaaacaag
     9361 tttcagaaga tggtggacaa gtcttattcg taggaactaa aaaacaagca caagaatcag
     9421 ttaaatctga agcagaacgt gctggtcaat tctacattaa ccaaagatgg ttaggtggat
     9481 tattaactaa ctataaaacg atctcaaaac gaatcaaacg tatttctgaa attgaaaaaa
     9541 tggaagaaga tggtttattc gaagtattac ctaaaaaaga agtagtagaa cttaaaaaag
     9601 aatacgaccg tttaatcaaa ttcttaggcg gaattcgtga tatgaaatca atgcctcaag
     9661 cattattcgt agttgaccca cgtaaagagc gtaatgcaat tgctgaagct cgtaaattaa
     9721 atattcctat cgtaggtatc gttgacacta actgtgatcc tgacgaaatt gactacgtta
     9781 tcccagcaaa cgacgatgct atccgtgcgg ttaaattatt aactgctaaa atggcagatg
     9841 caatcttaga aggtcaacaa ggcgtttcta atgaagaagt agctgcagaa caaaacatcg
     9901 atttagatga aaaagaaaaa tcagaagaaa cagaagcaac tgaagaataa tcaactgttg
     9961 aatctgactt agatatagtt taaatgggtg ataagatatt aatgcttatc acctttttta
    10021 aaaagaaaat cgaggcaaat tacaaatatt caattagagt attggcaatc ttgcctataa
    10081 taatgctaaa atcataatat ataaaatgat aacttattgg aggaataatg aatggcaact
    10141 atttcagcaa aacttgttaa agaattacgt gaaaaaactg gcgcgggtat gatggattgt
    10201 aaaaaagcgc taactgaaac tgatggtgac atcgataaag cgattgacta cctacgtgaa
    10261 aaaggtattg ctaaagcagc taaaaaagca gaccgtattg cggctgaagg tttagtacat
    10321 gtagaaacta aaggtaacga cgcagttatc gttgaaatca actctgaaac agactttgtt
    10381 gctcgtaacg aaggtttcca agagttagtt aaagaaatcg ctaatcaagt attagataca
    10441 aaagctgaaa ctgttgaagc tttaatggaa acaactttac caaatggtaa atcagttgat
    10501 gaaagaatta aagaagcaat ttcaacaatc ggtgaaaaat taagtgttcg tcgttttgct
    10561 atcagaacta aaactgataa cgatgctttc ggcgcttact tacacatggg tggacgcatt
    10621 ggtgtattaa cagttgttga aggttcaact gacgaagaag cagcaagaga cgttgctatg
    10681 catatcgctg caatcaaccc taaatatgtt tcttctgaac aagttagcga agaagaaatc
    10741 aaccacgaaa gagaagtttt aaaacaacaa gcattaaatg aaggtaaacc agaaaacatc
    10801 gttgaaaaaa tggtggaagg acgtttacgt aaatacttac aagaaatttg tgctgtagat
    10861 caagacttcg ttaaaaaccc tgatgtaaca gttgaagctt tcttaaaaac aaaaggtgga
    10921 aaacttgttg acttcgtacg ctatgaagta ggcgaaggta tggaaaaacg cgaagaaaac
    10981 tttgcggatg aagttaaagg acaaatgaaa taatctgtca taaagtaaaa caaggaagaa
    11041 gacaccttta atgttgcttt attaaaatgt aaatcattct aataaaacga caactgtgtc
    11101 ttctttactt gtatatgtta catatattca cgatagagag gataagaaaa tggctcaaat
    11161 ttctaaatat aaacgtgtag ttttgaaact aagtggtgaa gcgttagctg gagaaaaagg
    11221 atttggcata aatccagtaa ttattaaaag tgttgctgag caagtggctg aagttgctaa
    11281 aatggactgt gaaatcgcag taatcgttgg tggcggaaac atttggagag gtaaaacagg
    11341 tagtgactta ggtatggacc gtggaactgc tgattacatg ggtatgcttg caactgtaat
    11401 gaatgcctta gcattacaag atagtttaga acaattggat tgtgatacac gagtattaac
    11461 atctattgaa atgaagcaag tggctgaacc ttatattcgt cgtcgtgcaa ttagacactt
    11521 agaaaagaaa cgcgtagtta tttttgctgc aggtattgga aacccatact tctctacaga
    11581 tactacagcg gcattacgtg ctgcagaagt tgaagcagat gttattttaa tgggcaaaaa
    11641 taatgtagat ggtgtatatt ctgcagatcc taaagtaaac aaagatgcgg taaaatatga
    11701 acatttaacg catattcaaa tgcttcaaga aggtttacaa gtaatggatt caacagcatc
    11761 ctcattctgt atggataata acattccgtt aactgttttc tctattatgg aagaaggaaa
    11821 tattaaacgt gctgttatgg gtgaaaagat aggtacgtta attacaaaat aaatttagag
    11881 gtgtaaaata atgagtgaca ttattaatga aactaaatca agaatgcaaa aatcaatcga
    11941 aagcttatca cgtgaattag ctaacatcag tgcaggaaga gctaattcaa atttattaaa
    12001 cggcgtaaca gttgattact atggtgcacc aacacctgta caacaattag caagcatcaa
    12061 tgttccagaa gcacgtttac ttgttatttc tccatacgac aaaacttctg tagctgacat
    12121 cgaaaaagcg ataatagcag ctaacttagg tgttaaccca acaagtgatg gtgaagtgat
    12181 acgtattgct gtacctgcct taacagaaga acgtagaaaa gagcgcgtta aagatgttaa
    12241 gaaaattggt gaagaagcta aagtatctgt tcgaaatatt cgtcgtgata tgaatgatca
    12301 gttgaaaaaa gatgaaaaaa atggcgacat tactgaagat gagttgagaa gtggtactga
    12361 agatgttcag aaagcaacag acaattcaat aaaagaaatt gatcaaatga ttgctgataa
    12421 agaaaaagat attatgtcag tataaaacta atatacaatg acatattaaa atgccagtat
    12481 taaacgataa tgtaacattt aaaatgggca tgtttaatta aatcaaagat gcatgtgata
    12541 atttaaattc acaatgagca taaaaatggt gtttaaacaa gttaattaaa catatacttt
    12601 ataaataata ggcattaggt atattgctat aataaagtta tgtaattttt aacctcagta
    12661 tgtatgtcac atttctggtg taaactgtac cgagtcagac tttggtacag tttttttatt
    12721 tgcttattca atgcattaaa tgagtatgat aaaatgataa tgattgttta gtaacttata
    12781 ctatatgaca gagatgatca ggctcggagg aaagaccatg ttt
//
LOCUS       DAKTIO010000069        12797 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.069, whole genome shotgun sequence.
ACCESSION   DAKTIO010000069 DAKTIO010000000
VERSION     DAKTIO010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12797
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.069"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..1173
                     /locus_tag="O0293_001447"
     CDS             <1..1173
                     /locus_tag="O0293_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCY0280336.1"
                     /translation="FPGFEGEWEEKQLGDLTDRVIRKNKNLESKKPLTISGQLGLIDQ
                     TEYFSKSVSSKNLENYTLIKNGEFAYNKSYSNGYPLGAIKRLTRYDSGVLSSLYICFS
                     IKSEMSKDFMEAYFDSTHWYREVSGIAVEGARNHGLLNVSVNDFFTILIKYPSLEEQQ
                     KIGKFFSKLDRQIELEEQKLELLQQQKKGYMQKIFSQELRFKDENGEDYPDWENSKIE
                     KYLKERNERSDKGQMLSVTINSGIIKFSELDRKDNSSKDKSNYKVVRKNDIAYNSMRM
                     WQGASGKSNYNGIVSPAYTVLYPTQNTSSLFIGYKFKTHRMIHKFKINSQGLTSDTWN
                     LKYKQLKNINIDIPVLEEQEKIGDFFKKMDILISKQKMKIEILEKEKQSFLQKMFL"
     gene            1556..2233
                     /locus_tag="O0293_001448"
     CDS             1556..2233
                     /locus_tag="O0293_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL11"
                     /protein_id="HCY0280337.1"
                     /translation="MKLKNIAKASLALGILTTGMITTTAQPVKASTLEVRSQATQDLS
                     EYYNRPFFEYTNQSGYKEEGKVTFTPNYQLIDVTLTGNEKQNFGEDISNVDIFVVREN
                     SDRSGNTASIGGITKTNGSNYIDKVKDVNLIITKNIDSVTSTSTSSTYTINKEEISLK
                     ELDFKLRKHLIDKHNLYKTEPKDSKIRITMKDGGFYTFELNKKLQTHRMGDVIDGRNI
                     EKIEVNL"
     gene            2255..3763
                     /locus_tag="O0293_001449"
     CDS             2255..3763
                     /locus_tag="O0293_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKLRK protein"
                     /protein_id="HCY0280338.1"
                     /translation="MRENFKLRKMKVGLVSVAITMLYIMTNGQAEASENQNALISNIN
                     VDNQEKQNNVNQAVQPQNNTNETSKVPANFVKLNDIKPGDTSIQGTTLPNQFILLTID
                     KKDVSSVEDSDSSFVMSDKDGNFKYDLNGRKIVHNQEIEVSSSDPYLGDDEEDEEVEE
                     TSTEEVGAEEESTEAKATYTTPRYEKAYEIPKEQLKEKDGHHQVFIEPITEGSGIIKG
                     HTSVKGKVALSINNKFINFETNANGGPNKEEAKSGSEGIWMPIDDKGYFNFDFKTKRF
                     DDLELKKNDEISLTFAPDDEDEALKSLIFKTKVTSLEDIDKAETKYDHTKVEKVKVLK
                     DVKEDLHVDEIYGSLYHTEKGKGILDKEGTKVIKGKTKFANAVVKVDSELGEGQEFPD
                     LQVDEKGEFSFDVDHAGFRLQNGETLNFTVVDPITGELLSGNFVSKNIDIYESPEEKA
                     DREFDERMENTPAYHKLHGDKIVGYDTNGFPITWFYPLGEKKVERKAPKLEK"
     gene            complement(3866..4174)
                     /gene="spn"
                     /locus_tag="O0293_001450"
     CDS             complement(3866..4174)
                     /gene="spn"
                     /locus_tag="O0293_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myeloperoxidase inhibitor SPIN"
                     /protein_id="HCY0280339.1"
                     /translation="MKFKKVLVATAMVGVLATGVVGYGNQADAKVYSQNGLVLHDDAN
                     FLEHELSYIDVLLDKNADQATKDNLRSYFADKGLHSIKDIINKAKQDGFDVSKYEHVK
                     "
     gene            4542..5315
                     /gene="lpl1"
                     /locus_tag="O0293_001451"
     CDS             4542..5315
                     /gene="lpl1"
                     /locus_tag="O0293_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl1"
                     /protein_id="HCY0280340.1"
                     /translation="MGYLKRFALYISVMILIFAIAGCGKGNETKEDSKEEQIKKSFAI
                     TLDMYPIKNLEDLYDKEGYRDSEFKKGDKGMWTIYTDFAKSNKPGELDDEGMVLNLDR
                     NTRTAKGHYFVTTFYRNGKLPDEKNYKIEMKNNKIILLDEVKDDKLKQKIENFKFFGQ
                     YANLKELRKYNNGDVSINENVPSYDVEYKMSNKDEIVKELRSRYNISTEKSPILKMHI
                     DGDLKGSSVGYRKLEIDFSKRENSKLSVIEFLSYKPAKK"
     gene            5346..6146
                     /gene="lpl2"
                     /locus_tag="O0293_001452"
     CDS             5346..6146
                     /gene="lpl2"
                     /locus_tag="O0293_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl2"
                     /protein_id="HCY0280341.1"
                     /translation="MRYLKKLAWFISVIILGIFIIGCDSSSDTGEKAKEDSKEEQIKK
                     SFAKTLDMYPIKNLEDLYDKEGYRDSEFKKGDKGMWMIYTDFAKSNKPGVLDNEGMIL
                     NLDRNTRTAKGYYFVDTIYDNHENSYSKNYRVEMKNNKIILLDKVEDQKLKERIENFK
                     FFGQYADFKSLKSYNNGDVSINSNVPSYDAKFKMSNKDENVKQLRSRYNIPTEKAPIL
                     KMHIDGDLKGSSVGYKKLEIDFSKEENSELSVVDSLNFQPAKKNKDDE"
     gene            6165..6972
                     /locus_tag="O0293_001453"
                     /pseudo
     CDS             6165..6972
                     /locus_tag="O0293_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            7021..7821
                     /gene="lpl4"
                     /locus_tag="O0293_001454"
     CDS             7021..7821
                     /gene="lpl4"
                     /locus_tag="O0293_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl4"
                     /protein_id="HCY0280342.1"
                     /translation="MGYLKRLVLYIVIMVMSVFIIGCDKSSDTAEKSKEDSKETQIKK
                     SFAKTLDMYSIKNLEELYDKEGYRDGEFEKGDKGMWTIYTDFAKSNKPGELSNEGMVL
                     YLDRNTRTAKGYYFVRTFYRKDKLPDRKNYKVEMKNNKIILLDKVEDKKLKQKIENFK
                     FFSQYANLKELKNYSNGDVSINENVPSYDVKYKMSNKDENVKQLRSRYNIPTDKSPVL
                     KMHIDGNLKGSSVGDRKLEIDFSKRENSHLSVIDSLDYQPAKTNKDDE"
     gene            7840..8610
                     /gene="lpl5"
                     /locus_tag="O0293_001455"
     CDS             7840..8610
                     /gene="lpl5"
                     /locus_tag="O0293_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl5"
                     /protein_id="HCY0280343.1"
                     /translation="MKSIKRIGLCISLLILSIFVTSCDGDNKITGDSKETQIKKSFSK
                     TLDMYPIKNLEDLYDKEGYRDGEFKKGDKGMWTIYTDFAKGNKSDELDDEGMVLNLDR
                     NTRTAKGYYFVKKFYEKDKLPDRKNYKVEMKNNKIILLDKVEDPNLKKRIENFKFFGQ
                     YANFKDLENYNNGDVSINWNVPSYDVEYKMSNKDENVKQLRSRYNIPTDKAPMLKMHI
                     DGDLKGSSVGYKRLEIDFSKEGRDISVIDYLSYKPAKK"
     gene            8642..9433
                     /gene="lpl6"
                     /locus_tag="O0293_001456"
     CDS             8642..9433
                     /gene="lpl6"
                     /locus_tag="O0293_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl6"
                     /protein_id="HCY0280344.1"
                     /translation="MEYLKRLALLISVIILTIFIMGCDSQSDTAENPKEGSKEAQIKK
                     SFSKTLDMYPIKNLEDFYGKEGYRDGEFKKDDKGTWLIRSEIVKQPKGKVMKTRGMQL
                     YINRNTETAKGFFVLKEISENNNRVNKDKEEKYEVKMVGNKIIPTEQINDEKIKKEIE
                     NFKFFVQYGNFKNFEKYNNGEFSYNPEAPIYSAKYQLHNDDYNVRQLRKRYDISTKET
                     PKLLLKGGGDLKNSSVGQNDIEFTFVERKGENIYFNDSVEFIPSK"
     gene            9503..10279
                     /gene="lpl7"
                     /locus_tag="O0293_001457"
     CDS             9503..10279
                     /gene="lpl7"
                     /locus_tag="O0293_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl7"
                     /protein_id="HCY0280345.1"
                     /translation="MKCFQKLYIFILILIVLMAGCESNKITGDSKETQIKKSFAKTLD
                     VYPTKNLEDFYDKEGYRDGEFKKGDKGKWVIRSEMTTELKNENMVSKGMVIRLNRNSR
                     TCTGEYFVRIVKEDSEGKVYSDERKYPVKMENNKIITLKPIDDEKVKKEIEEFKFFVQ
                     YGNFKELENYKDGEVTYNPEAPIYSAQYQLKNSDYNVEQLRKRYNITTKKAPKLLLKG
                     SGNLKGSSVGYKNIEFTFVENKEENIYFTDSINFNPSEDK"
     gene            10458..11270
                     /gene="lpl8"
                     /locus_tag="O0293_001458"
     CDS             10458..11270
                     /gene="lpl8"
                     /locus_tag="O0293_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl8"
                     /protein_id="HCY0280346.1"
                     /translation="MGYIKRMTLYMSVFLLIIFIVGCRNMKDEQKKEEQTNKTDSKEE
                     QIKKSFEKTLDMYPIKNLEELYDKEGYRDGEFKKGDKGMWTIYTDFAKSNKQGGLSNE
                     GMVLYLDRNTRTAKGHYFVKTFYNKGKFPDRKNYKVEMKNNKIILLDKVEDTNLKKRI
                     ENFKFFGQYANLKELKNYNNGDVSINENVPSYDAKFKMSNKDENVKQLRSRYNIPTDK
                     APVLKMHIDGNLKGSSVGYKKLEIDFSKGGKSDLSVIDSLNFQPAKVDEDDE"
     gene            11289..12074
                     /gene="lpl9"
                     /locus_tag="O0293_001459"
     CDS             11289..12074
                     /gene="lpl9"
                     /locus_tag="O0293_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl9"
                     /protein_id="HCY0280347.1"
                     /translation="MKSIKRIGLCISLLILIIFVTSCDGDNKIIGDSKEEQIKKSFAK
                     TLDIYPIKNLEDLYDKEGYRDGEFKKDDKGTWLIRSEMKIQLKGENLESRGAVLEINR
                     NTRTAKGHYIVREVVEDSDGMTHNHTKRYPVKMENNKMIPLKPIDDEKVKKEIEEFNF
                     FVQYGNFKELENYKEDEVSYNPEVPIYSAKYQLKNSDYNVEQLRKRYNIPTQKAPKLL
                     LKGSGNLKGSSVGYKNIEFTFIENKEENIYFTDSIYFNPSEDK"
     gene            12257..>12797
                     /gene="lpl10"
                     /locus_tag="O0293_001460"
     CDS             12257..>12797
                     /gene="lpl10"
                     /locus_tag="O0293_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl10"
                     /protein_id="HCY0280348.1"
                     /translation="MEYIKKIALYMSVLLLIIFIGGCGNMKDEQKKEEQTNKTDSKEE
                     QIKKSFAKTLDMYPIKNLEDLYDKEGYRDGEFKKGDKGTWTILTGFSKSNKPGVLDDE
                     GMVLYLNRNAKKATGYYFVNKVYDDISKNHNEKKYRVELKNNKIVLLDNVEDKKLKQK
                     IENFKFFSQYADFKDLKNYQ"
BASE COUNT         5377 a         1365 c         2364 g         3691 t
ORIGIN      
        1 ttcccagggt ttgaaggcga atgggaagag aagcagttag gggatcttac agatagagta
       61 attaggaaaa ataaaaactt agaatcgaaa aagcctttaa caatatccgg acagttaggt
      121 ttaattgatc aaacagaata ttttagtaaa tcagtttcgt cgaaaaatct agaaaattat
      181 acactaataa agaatggaga attcgcgtat aacaaaagtt attctaatgg atacccatta
      241 ggggctatta aaagattaac tagatatgat agtggtgtat tgtcctcttt gtatatttgt
      301 ttttctatta aaagtgaaat gtctaaagac ttcatggaag catattttga ttcgacacac
      361 tggtatagag aagtttctgg aattgcagtt gagggtgcaa gaaatcacgg attattaaat
      421 gtttctgtga atgatttttt tactattcta attaaatatc caagtttaga agaacagcaa
      481 aaaataggca agttcttcag caaactcgac cgacaaattg aattagaaga acaaaagctt
      541 gaattacttc aacaacagaa aaaaggctat atgcagaaaa ttttctcaca ggaactgcga
      601 ttcaaagatg agaatggtga agattatcca gattgggaaa atagcaaaat agaaaaatat
      661 ttaaaagaga gaaacgaacg ttctgacaaa gggcaaatgc tttcagtaac tataaatagt
      721 ggcattataa aatttagtga attggataga aaagataatt caagtaaaga taaaagtaat
      781 tataaagtag ttaggaaaaa tgatattgca tataattcta tgagaatgtg gcaaggggct
      841 agtggtaaat caaattataa tgggattgtt agccctgcat atactgtgct ttatccaaca
      901 caaaatacta gctcattatt tattggatat aagtttaaaa cacatagaat gattcataaa
      961 tttaaaatta attcacaagg attaacatca gatacatgga acttaaaata taaacaatta
     1021 aaaaatataa atatagatat acctgtattg gaggaacaag aaaagatagg tgatttcttt
     1081 aaaaaaatgg atatattgat aagtaaacag aaaatgaaaa ttgaaatatt agaaaaagag
     1141 aaacaatcct ttttacaaaa aatgttctta taactttgat aaatacatag attgcataag
     1201 aataaaattt gtataattta acataaaagt tgtaaaagta aagtgaatta aaaacgaaca
     1261 ttaaatttag gcactgtgaa agcgcagtgt cttttttgtg tcgaaattgt gtacagaata
     1321 agtagttaaa taaagattaa gttgagataa agtgttattc gtaaataaaa gagagtagat
     1381 cgataggaat tgaatgatat tagttaacta tttattaaat tacttaataa tgattaattt
     1441 ttagttaaag taagtttaat gtgaagcacg accattgctc attataatga atgaggattg
     1501 ttcgtattgc gtaatagaat aaatcaaata gactaaaaat tgggagcata gaattatgaa
     1561 attaaaaaat attgctaaag caagtttagc actagggatt ttaacaacag ggatgattac
     1621 aactactgct cagccagtaa aagcaagtac attagaggtt agatcacaag ctactcaaga
     1681 cttgagtgaa tattataata gaccgttctt tgagtataca aatcagtcag gatataaaga
     1741 ggaaggaaaa gtgacgttta ctcctaatta tcaacttata gatgtaactt taactgggaa
     1801 tgaaaagcaa aattttggtg aagatatttc taatgtagat atatttgttg taagagaaaa
     1861 ttctgataga tctggtaata cagcttcaat tggtggtatt actaaaacaa acggttcaaa
     1921 ttatattgat aaagtaaaag atgtaaattt aataattact aaaaacatcg atagtgttac
     1981 atcaacgtca acatcatcta catatacaat taataaagaa gaaatttcat taaaagaact
     2041 tgattttaaa ttaagaaagc atttaattga taaacataac ctttataaga cagaacctaa
     2101 agacagtaaa attcgaatta ctatgaaaga tggtgggttc tacacatttg aattgaataa
     2161 aaagttacaa acacaccgta tgggtgatgt tattgatggc agaaatatag aaaaaattga
     2221 agtgaattta taaaattatt cgagggagca tatcatgagg gaaaatttta agttacgtaa
     2281 aatgaaagtc ggtttagtat ctgttgcaat tacaatgtta tatattatga caaacggaca
     2341 agcagaagca tctgaaaatc aaaacgcttt aatctctaat ataaatgtag acaatcagga
     2401 aaaacagaat aatgtaaatc aagctgttca gcctcaaaat aatactaatg aaacatcaaa
     2461 agtaccggct aattttgtca aattgaatga tattaaacca ggtgatactt ctatacaagg
     2521 aacaacttta ccaaatcaat ttatactatt aactattgat aaaaaagatg tgagctcagt
     2581 tgaagattct gacagcagct ttgttatgtc tgataaagat gggaatttta agtatgactt
     2641 aaatggtcgc aaaattgttc ataatcaaga aattgaagtg tcttcatcag atccctattt
     2701 aggtgacgat gaagaagatg aagaagtaga agaaacttca actgaagaag ttggtgctga
     2761 ggaagaaagt acagaagcta aagctacata tacaacaccg cgatatgaaa aagcgtatga
     2821 aataccgaaa gaacagctaa aagaaaaaga tggacatcac caagttttta tcgaacctat
     2881 tactgaaggt tcaggtatta ttaaaggcca tacctctgta aaaggtaaag ttgctctatc
     2941 tattaataat aaatttatta actttgagac aaatgctaat ggtggtccaa ataaagaaga
     3001 agcgaaatct ggatcagaag gaatctggat gcctattgat gacaaaggat actttaattt
     3061 tgacttcaaa acgaaacgtt tcgatgattt agagttaaag aaaaatgatg agatctcatt
     3121 aacatttgca cctgatgacg aagatgaggc attgaagtca ttaattttca aaactaaggt
     3181 aacgagttta gaagatattg ataaagcaga aactaaatat gaccatacta aagtggaaaa
     3241 agtaaaagta ttgaaagatg ttaaagaaga tttacatgta gatgaaattt acggaagctt
     3301 atatcataca gaaaaaggta aaggtattct tgataaagaa ggtactaaag taattaaagg
     3361 taagactaaa ttcgcaaatg cagttgtgaa ggtagactct gaactaggtg aaggtcaaga
     3421 attccctgat ttgcaagtcg atgaaaaagg tgaattcagc tttgatgtag atcatgctgg
     3481 atttagatta caaaatggag aaacactaaa cttcacagta gttgatccta ttacaggtga
     3541 attattaagt ggaaattttg tttctaaaaa catagatatt tatgaatctc ctgaagaaaa
     3601 agcagatcgt gagtttgatg aaagaatgga aaacacacct gcatatcata aattacatgg
     3661 tgataaaatt gtcggctacg atactaacgg attcccgatt acctggtttt atccattagg
     3721 tgaaaagaaa gttgaacgta aggcaccaaa attagaaaaa taattaaata aaacagctta
     3781 atgatgtaat gaaattagtg agttaatcac tgacttctac gtcattgagc tgttttttgt
     3841 gctttgttac aaagcattat tgaatttatt ttacgtgttc atattttgaa acatcaaagc
     3901 cgtcttgttt agctttgttg ataatgtctt tgattgaatg tagtccttta tcggcgaagt
     3961 atgatcttaa gttgtctttt gtagcttggt cagcattctt atctaataac acatcaatat
     4021 aacttaattc atgttctaag aagtttgcat catcatgtag tacgagtcca ttttgagaat
     4081 aaactttcgc atctgcttga ttaccatatc caacaacgcc agttgctaaa acacctacca
     4141 ttgccgtagc tactaaaacc tttttaaatt tcatatctat cactcctcta aaaattgtaa
     4201 ctccatcata acactgaata ttaagaaaat tacgtttatt aagtcgattt aataattttt
     4261 aataaatagt taaagtgaca aatattgttt aaatgcaatt aatctttaat acgatgcttg
     4321 aggatttttc ctaataaaac cttgatttca aaaagggttt aaatcaaatg aaacaataat
     4381 aaaaaaatga cgcaatataa taataagtac aaatttaatt aagaaattaa attgattgta
     4441 tatgtatatt ttggtaacgt aaaagagaaa tatacaaaat aattaattat ttatatgaaa
     4501 agagaatata aatgaagtat aaaacagaga gacgtgaagc gatgggatat ttaaaaaggt
     4561 ttgcattgta cataagcgtt atgattttaa tatttgcgat agcaggttgt ggcaaaggta
     4621 atgaaacaaa agaagattca aaggaagaac aaatcaaaaa gagctttgcg ataacattag
     4681 atatgtatcc aattaagaat ctcgaggact tatatgacaa agaaggatat cgagatagcg
     4741 aatttaaaaa aggtgacaaa gggatgtgga cgatatatac agatttcgcc aaaagtaata
     4801 aaccgggtga attggatgat gaaggtatgg ttttaaatct ggataggaat actagaaccg
     4861 ctaaaggcca ttattttgtt actacatttt accggaatgg taaactacca gatgaaaaga
     4921 attataaaat tgaaatgaaa aataataaga ttattttatt agatgaagta aaagatgata
     4981 agctcaagca gaaaatagaa aactttaaat ttttcggtca atatgcaaat cttaaagaat
     5041 tgagaaaata taataatggt gatgtttcaa ttaatgaaaa tgttcctagt tatgatgttg
     5101 aatacaaaat gagcaataaa gatgaaatag ttaaggaatt aagaagtcgt tataatattt
     5161 cgactgaaaa atcacctata ttaaaaatgc atattgatgg ggacctgaaa ggcagttctg
     5221 taggttatag aaagttagaa attgactttt caaaacgtga aaacagcaaa ttatcagtca
     5281 ttgaattttt aagttataaa ccagcgaaaa aatagtattg ataaggaata ttagggtgtg
     5341 aaaaaatgcg atatctaaaa aagcttgcat ggttcataag tgttattatt ttgggcattt
     5401 ttataatagg ttgtgatagt tcaagcgata ctggggaaaa agcaaaagaa gattcaaagg
     5461 aagaacaaat caaaaagagc tttgcgaaaa cattagatat gtatccaatt aagaatctcg
     5521 aggacttata tgacaaagaa ggatatcgag atagcgaatt taaaaaaggt gacaaaggga
     5581 tgtggatgat atatacagat ttcgccaaaa gtaataaacc gggtgtatta gataatgaag
     5641 gtatgatttt aaatttggat agaaatacac gtacggccaa gggatattat tttgtagata
     5701 ctatatatga caatcatgaa aactcttata gtaaaaatta tagagttgag atgaaaaaca
     5761 ataaaattat tttattagac aaggtggaag atcaaaaact taaagaaaga atagaaaact
     5821 ttaaattttt cggacaatat gccgatttca agagtttgaa aagttacaac aatggcgacg
     5881 tttcaattaa tagtaatgtt ccaagttatg acgcgaaatt taaaatgagt aataaagatg
     5941 aaaatgttaa gcaattaaga agtcgttata acattcctac tgaaaaagct ccaatattaa
     6001 aaatgcatat tgatggggac ttaaaaggca gttccgttgg atataaaaag ttagaaatag
     6061 acttttcaaa agaagaaaat agcgaattat cagtagtcga ttcattaaat tttcagcctg
     6121 ccaaaaaaaa taaagatgat gaatgatagg gaggatgtga aacaatgtgg tcaataaaaa
     6181 gaattggaat gtacacaaat gttataattt tgagcgtttt tataataggt tgtgatagtt
     6241 caagcaataa tgcagaaaat caaagagaag actcaaaaga agaacaaatc aaaaagagct
     6301 ttgcgaaaac gttagatatg taccctatta agaatctcga ggatttatat gacaaagaag
     6361 gatatcgtga tggtgagttt gaaaaaggcg ataaggggac gtgggtattg tattcagcta
     6421 tggtatcaca accaaaaggc gagagtttaa aatcacgagg aatgatatta aagttagata
     6481 gaaataagag aactgctaaa ggaagttata ttattagaga attgaaagaa gataaaaatc
     6541 atgatgttca aaaaaatgaa aagaaatatc cagtgaaatt ggtgaataat aggatagttt
     6601 tgataaaaga tgttaaagac aaaaagttaa aaaaatgaaa tagagtcgtt tgaattattt
     6661 tcacaatatg gaaactttaa tcattttgat cggaatgaga ttactaatat ttcatataat
     6721 cctaatgctc ccaattactc tgcagaatat aaaatgaaga aaaatgacag aaacattcaa
     6781 cagttgaaaa agagatttaa tctaaaaact agcaagacac caaaattatt gtttaaggga
     6841 tctggagata taaaggggtc ttctgtagga tataaggaaa tagaaatcat atttagtaga
     6901 agtaaagaag aaagcattta ttatgttgac agcgttgagt tcgttccaag taacaaaata
     6961 acaaaaagtt gatattagtc aacaaaagca aatattgaga ataaagagag ttgtgaaatg
     7021 atgggttatt taaaaagact tgtattgtat atagttatta tggttatgag tgtttttata
     7081 ataggttgtg ataaatcaag cgatactgca gaaaaatcaa aagaagactc gaaagaaaca
     7141 caaatcaaaa agagttttgc gaaaacgtta gatatgtatt caattaagaa tctcgaggaa
     7201 ctatatgaca aagaaggcta tcgagatggc gaatttgaaa aaggtgacaa agggatgtgg
     7261 acgatatata cagatttcgc caaaagtaat aaaccgggtg aactaagtaa tgaaggcatg
     7321 gtcttgtact tagatagaaa cacacgcaca gctaaggggt actactttgt caggacgttt
     7381 tatcgcaagg ataaattacc tgatagaaaa aattataaag ttgaaatgaa aaataataaa
     7441 atcattttat tagataaagt agaagataaa aagcttaaac aaaaaataga aaattttaaa
     7501 tttttcagtc aatatgcaaa tcttaaagaa ttgaaaaact acagcaatgg tgatgtctca
     7561 attaatgaga atgttccaag ttatgacgtg aaatataaaa tgagtaataa agatgaaaat
     7621 gttaagcaat taagaagtcg ttataatatt cctactgata aatcaccggt attaaaaatg
     7681 catattgatg gtaatttgaa aggaagttcc gttggagata ggaagttaga aattgatttt
     7741 tcgaaacgtg aaaatagcca tttatcagta atagattctt tggattacca gccagcgaaa
     7801 actaataaag atgatgaata atgaggatgg tgtgtaacaa tgaagtctat aaaaaggatt
     7861 ggattgtgca taagtctgtt gattttaagc atttttgtaa catcttgcga tggtgataat
     7921 aagatcactg gagattcaaa agaaacacaa atcaaaaaga gcttttcgaa aacgttagat
     7981 atgtatccaa ttaagaatct cgaggactta tatgataaag aaggataccg tgatggcgaa
     8041 tttaaaaagg gcgacaaagg aatgtggact atatatacag attttgctaa aggcaataaa
     8101 tcagacgaat tggatgatga aggtatggtt ttaaatctgg atagaaatac tcgaacggct
     8161 aagggatatt attttgttaa gaaattttat gaaaaggata aattacctga tagaaaaaat
     8221 tataaagttg aaatgaaaaa taataaaatt atcttattag acaaggtaga agatccaaat
     8281 ctaaaaaaga gaatagaaaa ctttaaattt ttcggacaat atgcaaattt taaggatttg
     8341 gaaaattaca acaatggcga cgtgtcaata aattggaatg ttccaagtta tgacgtggaa
     8401 tataaaatga gcaataaaga tgaaaatgtt aaacaattaa gaagtcgtta taacattcct
     8461 actgataaag ctccaatgtt aaaaatgcat attgacgggg acttaaaagg tagttctgtt
     8521 ggatataaaa ggttagaaat agatttttca aaagaaggta gggatatttc agtcattgat
     8581 tatttaagtt ataagccagc gaaaaaatag tactgataat taaatattag ggtgtgaagg
     8641 aatggaatat ctaaaaaggc ttgcattgtt aataagtgtt attattttga ccatttttat
     8701 aatgggttgt gatagtcaaa gcgatactgc agaaaatcca aaagaaggtt caaaagaagc
     8761 acaaattaaa aagagttttt cgaaaacgtt agatatgtat ccaattaaga atctcgagga
     8821 tttttatggc aaagaaggat atcgagatgg cgaatttaaa aaagatgata aaggtacttg
     8881 gctaattaga tccgaaatag ttaaacagcc aaagggcaaa gtgatgaaaa caagaggtat
     8941 gcaattatat attaatagaa ataccgaaac agccaaaggt ttctttgttt tgaaagaaat
     9001 aagtgaaaat aataatcgtg taaataaaga taaggaggaa aaatacgaag tgaaaatggt
     9061 aggaaataaa attattccta ctgaacaaat taatgacgag aaaataaaaa aagaaattga
     9121 aaacttcaag ttttttgtgc aatatggaaa ctttaaaaat ttcgaaaaat acaacaatgg
     9181 tgagttttca tataatcctg aagcaccaat ttattcggct aaatatcaat tacacaacga
     9241 tgattacaat gtaaggcaac tacgtaaaag atatgacatt tcaacaaaag aaacaccgaa
     9301 gttacttttg aaaggtggag gagatttaaa aaattcctca gttggtcaaa acgatattga
     9361 atttactttt gttgaaagaa aaggtgagaa tatttatttt aacgatagtg ttgaattcat
     9421 accaagtaag taaagttaaa ttaaaatgtg tggttagatg aaatgatcaa atatcaaaaa
     9481 atgtgcaaag agatgtgaca agatgaagtg ttttcagaaa ttatacatat ttatattaat
     9541 tttaatcgta ttaatggcag gatgcgaaag taataagatc actggagatt cgaaagaaac
     9601 acagatcaaa aagagctttg cgaaaacatt agatgtatac cctacgaaaa atctagaaga
     9661 tttttatgac aaagaaggat atcgagatgg cgaatttaaa aagggtgaca aagggaagtg
     9721 ggttattaga tctgaaatga caacagaact gaaaaatgaa aatatggtat ctaaaggtat
     9781 ggtcatacgt ttaaatagaa atagtagaac atgcactggt gaatattttg tcaggatagt
     9841 taaagaagac agtgagggca aggtatatag tgatgaacga aaatatccag tgaaaatgga
     9901 aaataataaa atcattacat taaaaccaat cgatgatgaa aaagtaaaaa aagaaattga
     9961 agaatttaaa ttcttcgtac aatacgggaa tttcaaagaa ttggaaaact ataaagacgg
    10021 agaagtgaca tataacccag aagcaccaat atactctgca caatatcaat tgaaaaatag
    10081 tgattataat gtagaacaac tacgtaagcg atataatata acgacgaaaa aagcgcctaa
    10141 attattattg aagggttcag gtaatttaaa aggctcatca gttggatata aaaatattga
    10201 atttaccttt gttgaaaata aggaagaaaa tatttacttc acagatagta ttaatttcaa
    10261 cccaagtgag gataaataag tatatttagt attacaaaaa cgttagaaaa attaataata
    10321 aagttaaatt aacaaactgt attaaattca tatagtcagt tttgtttgat gacatgatag
    10381 tagaaaaatt attattttaa taggttgtat gaactataca aatatttata aaaattaaaa
    10441 cagagagatg tgaaatgatg gggtatataa aaagaatgac tttatacatg agtgtatttc
    10501 ttttaatcat ttttattgtt ggatgtcgaa atatgaaaga tgaacagaaa aaagaagaac
    10561 aaacaaataa aacagattca aaagaagaac aaatcaaaaa gagttttgag aaaacattag
    10621 atatgtatcc aattaagaat ctcgaggagt tatacgacaa agaaggatac cgagatggcg
    10681 aatttaaaaa gggtgataaa gggatgtgga cgatatatac agatttcgcc aaaagtaata
    10741 aacaaggtgg attgagtaat gaaggtatgg tcttatactt agatagaaat acacggactg
    10801 caaagggaca ttattttgtt aagacattct ataataaggg caaattccca gatagaaaaa
    10861 attataaagt tgaaatgaaa aataataaaa ttatcttatt agataaagta gaagatacaa
    10921 atctaaaaaa gagaatagaa aactttaaat tttttggaca atatgcaaac cttaaagaat
    10981 tgaaaaacta caacaatggt gatgtctcaa ttaatgagaa tgttccaagt tatgacgcaa
    11041 aatttaaaat gagcaataaa gatgaaaatg ttaagcaatt aagaagtcgt tataatattc
    11101 ctactgataa agcaccggta ttaaaaatgc atattgatgg taatttgaaa ggaagttctg
    11161 tgggttataa aaagttggaa attgactttt caaaaggtgg aaaaagcgat ttgtcagtaa
    11221 tagattcttt gaatttccag ccggcgaagg tagatgaaga tgatgaatga tgaggatggt
    11281 gtgtaacaat gaagtctata aaaaggattg gattgtgcat tagtttgttg attttaatca
    11341 tctttgttac atcttgtgat ggtgataata agatcattgg agattcaaaa gaagaacaaa
    11401 tcaaaaagag ctttgcgaaa acgttagata tctaccctat taagaatctc gaggatttat
    11461 acgacaaaga aggatatcga gatggcgaat ttaaaaaaga tgataaaggt acttggctaa
    11521 ttagatctga aatgaaaatc caattaaaag gagaaaatct ggaatctaga ggagcagttt
    11581 tagaaattaa cagaaatact agaacggcta aagggcatta tattgttaga gaagttgttg
    11641 aagatagcga tggaatgaca cacaatcata caaaaagata tcctgtgaaa atggaaaata
    11701 ataaaatgat tccattaaaa ccaatcgatg acgaaaaagt aaaaaaagaa atcgaagaat
    11761 ttaacttctt tgtacaatac gggaatttca aagaattgga aaactataaa gaggacgaag
    11821 tgtcatataa cccagaagta ccaatttact ctgcaaaata tcaattgaaa aacagtgatt
    11881 acaatgttga acaattaagg aagcgatata atatcccgac gcaaaaagcg cccaaattat
    11941 tattgaaagg ctcaggtaat ttaaaaggtt catcagtcgg atataaaaat attgaattta
    12001 cctttattga aaacaaggaa gaaaatattt acttcacaga tagtatctac tttaatccaa
    12061 gcgaggataa ataaatataa ttactaatat aaaaaataac gttagaacat ttaataataa
    12121 agttaaaatg aattacatgt attaaattga ataagttagt tttgtttgat aacataaaag
    12181 ttgaataatc actattttaa taagttgtat gaaatattca aatgtttata aaattaaaac
    12241 agagagatgt gaaatgatgg agtatataaa aaaaattgct ttgtacatga gtgtattact
    12301 tttaatcatt tttattgggg gatgtggaaa tatgaaagat gaacagaaaa aagaggaaca
    12361 aacgaataaa acagattcaa aagaagaaca aatcaaaaag agttttgcga aaacgttaga
    12421 tatgtatcca attaagaatc tcgaggattt atacgacaaa gaaggatatc gagatggtga
    12481 gtttaaaaaa ggcgataaag gtacgtggac tatacttaca ggtttttcaa aaagtaacaa
    12541 accaggagta ttagatgatg aaggcatggt gttatatctt aatagaaatg ccaaaaaggc
    12601 aacaggttat tattttgtaa ataaagttta tgatgatatt agcaaaaatc ataatgagaa
    12661 aaaatatcgt gttgaactaa aaaataataa gattgttctt ttggataatg tagaagacaa
    12721 aaaacttaaa caaaaaattg aaaattttaa attttttagt cagtatgctg attttaaaga
    12781 tttaaaaaat tatcaag
//
LOCUS       DAKTIO010000070        12787 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.070, whole genome shotgun sequence.
ACCESSION   DAKTIO010000070 DAKTIO010000000
VERSION     DAKTIO010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12787
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.070"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            53..1021
                     /gene="dhaK"
                     /locus_tag="O0293_001461"
     CDS             53..1021
                     /gene="dhaK"
                     /locus_tag="O0293_001461"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCY0280349.1"
                     /translation="MMKKLINKKETFLTDMLEGLLIAHPELDLIANTVIVKKAKKEHG
                     VAIVSGGGSGHEPAHAGFVAEGMLDAAVCGEVFTSPTPDKILEAIKAVDTGDGVLLVV
                     KNYAGDVMNFEMAQELAEMEGINVQTVIVRDDIAVTNEVQRRGVAGTVFVHKLAGYLA
                     EKGYSLTEIKSRVEALLPEIKSIGMAIEPPLVPTTGKYGFDIEDDKMEIGIGIHGEKG
                     IHREEVKDIDHIVGTLLDELYKEVTANDVILMVNGMGGTPLSELNIVTKYIQQNLAAR
                     TVNVAKWFVGDYMTSLDMQGFSITIVPNKPEYLEAFLAPTTSQYFK"
     gene            1063..1647
                     /gene="dhaL"
                     /locus_tag="O0293_001462"
     CDS             1063..1647
                     /gene="dhaL"
                     /locus_tag="O0293_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HCY0280350.1"
                     /translation="MKVNDMKARLLNLEETFKKHESELTELDRAIGDGDHGVNMVRGF
                     SSLKDKLDDSSMQSLFKSTGMALMSNVGGASGPLYGFSFVKMSAVTKDDMDNQDFITL
                     IQAFAEAVESRGKVTLNEKTMYDVVARAAEKLKNGETLTFNDLQQLADNTKDMVATKG
                     RAAYFGEESKGYIDPGAQSMVYILNALIGDEDNA"
     gene            1640..2002
                     /locus_tag="O0293_001463"
     CDS             1640..2002
                     /locus_tag="O0293_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HCY0280351.1"
                     /translation="MPKIILVSHSKEIASGTKSLLKQMAGDVDIIPIGGLPDGSIGTS
                     FDIIQEVLTKLEDDALCFYDIGSSEMNVDMAIEMYDGNHRVLKVDAPIVEGSFIAAVK
                     LSIGGSIDDALAEIKQSF"
     gene            2118..2615
                     /locus_tag="O0293_001464"
     CDS             2118..2615
                     /locus_tag="O0293_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280352.1"
                     /translation="MQHLIKKHVLNGEFDLVRQLMSETDFMEFEEAYISSAHEVESMM
                     FYTCILDMIKYEESSEMHDLAFLLLVYPLSEYEGALDSAYYHADASIKLTDGKEVKSL
                     LQMLLLHAIPTPVISDKKAFDIAKQILKLDPNNNVARNVLKDTAKRMDNVVVDINELH
                     QRNAR"
     gene            3071..4138
                     /locus_tag="O0293_001465"
     CDS             3071..4138
                     /locus_tag="O0293_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280353.1"
                     /translation="MMMNKEATKIGFAYVGIVVGAGFSTGQEVMQFFTKYGLWAYLGV
                     IISGFILAFIGRQVAKIGTAFEATNHESTLQYVFGEKFSKVFDYILIFFLFGIAVTMI
                     AGAGATFEESYNIPTWLGALIMTLAIYITLLLDFNKIVRALGIVTPFLIVLVVLIAGV
                     YLFKGHVSLAEVNQVVPEASIWKGIWFGTIYGGLAFSVGFSTIVAIGGDTEKRTVSGA
                     GAMYGGIIYTVLLALINFALQSEYPTIKNASIPTLTLANNIHPLIATVLSVIMLAVMY
                     NTILGLMYSFAARFTEPYSKNYHIFIIIMMVAGYLLSFVGFAELINKLYTIMGYVGLF
                     IVVAVIIKYFKRKNADKKHIA"
     gene            4289..5332
                     /locus_tag="O0293_001466"
     CDS             4289..5332
                     /locus_tag="O0293_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0280354.1"
                     /translation="MNKDNKWTMITALFITVISVLLAFHLKQHYDQITNENHANKDKI
                     NIKNKNVRIYQNLTYNRVFPNSKLDIITPVDMSSNAKLPVIFWMHGGGYIAGDKQYKN
                     PLLAKIAEQGYIVVNVNYALAPQYKYPTPLIQMNQATQFIKENKMNLPIDFNQVIIGG
                     DSAGAQLASQFTAIQTNDRLREAMKFDQSFKPSQIKGAILFGGFYNMQTVRETEFPRI
                     QLFMKSYTGEEDWEKSFKNISQMSTVKQSTKNYPPTFLSVGDSDPFESQNIEFSKKLQ
                     ELNVPVDTLFYDGTHHLHHQYQFHLNKPESIDNIKKVLLFLSRNTSSSGIQTEEKPQI
                     ENPSNELPLNPLN"
     gene            5662..6090
                     /locus_tag="O0293_001467"
     CDS             5662..6090
                     /locus_tag="O0293_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280355.1"
                     /translation="MTRSALKPFKNKRVMVTGRIQRVLFKNYLDRHSTFKPNVRILLK
                     DVFVSGVSIDHLWLYETNKYYALAMELIHQRVKFSANVVPYYKINRNNNLFVQDYGIK
                     RKGRLITEEAYNQNNQYQDKIYEKLPDIDFRLEDFYSKEN"
     gene            complement(6299..6805)
                     /locus_tag="O0293_001468"
     CDS             complement(6299..6805)
                     /locus_tag="O0293_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0280356.1"
                     /translation="MAHIIRRVSIKDVENFISMLANIYDESPYMFYTPGEYDPSVTSA
                     SKQLEEYITSPHKVIFVAESDEQLVGFAFVNTTPFQRIKHVAKIDLGVKKLYQHRGIG
                     QALLDAIMAWCLNNQIHRIEANVPLNNQPALELFKSADFQIEGVLKDKLFIDGKYYDD
                     YMMAKILN"
     gene            6918..7841
                     /gene="graX"
                     /locus_tag="O0293_001469"
                     /gene_synonym="apsX"
     CDS             6918..7841
                     /gene="graX"
                     /locus_tag="O0293_001469"
                     /gene_synonym="apsX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="auxiliary protein GraX/ApsX"
                     /protein_id="HCY0280357.1"
                     /translation="MKPKVLLAGGTGYIGKYLSEVIENDAELFAISKYPDNKKTDDVE
                     MTWIQCDIFHYEQVVAAMNQIDIAVFFIDPTKNSAKITQSSARDLTLIAADNFGRAAA
                     INQVKKVIYIPGSRYDNETIERLGAYGTPVETTNLVFKRSLVNVELQVSKYDDVRSTM
                     KVVLPKGWTLKNVVNHFIAWMGYTKGTFVKTEKSHDQFKIYIKNKVRPLAVFKIEETA
                     DGIITLILLSGSLVKKYTVNQGKLEFRLIKESAVVYIHLYDYIPRLFWPIYYFIQAPM
                     QKMMIHGFEVDCRIKDFQSRLKSGENMKYTK"
     gene            7857..8531
                     /gene="graR"
                     /locus_tag="O0293_001470"
                     /gene_synonym="apsR"
     CDS             7857..8531
                     /gene="graR"
                     /locus_tag="O0293_001470"
                     /gene_synonym="apsR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor
                     GraR/ApsR"
                     /protein_id="HCY0280358.1"
                     /translation="MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFN
                     PEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSRDNPMDQVMSMELGADDYMQKPF
                     YTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAVVDLSKDSIQKGDQTIFLSKTEMIIL
                     EILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGK
                     GYMAHE"
     gene            8524..9564
                     /gene="graS"
                     /locus_tag="O0293_001471"
                     /gene_synonym="apsS"
     CDS             8524..9564
                     /gene="graS"
                     /locus_tag="O0293_001471"
                     /gene_synonym="apsS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase GraS/ApsS"
                     /protein_id="HCY0280359.1"
                     /translation="MNNLKWVAYFLKSRMNWIFWILFLNFLMLGISLIDYDFPIDSLF
                     YIVSLNLSLTMIFLLLTYFKEVKLYKHFDKDKEIEEIKHKDLAETPFQRHTVDYLYRQ
                     ISAHKEKVVEQQLQLNMHEQTITEFVHDIKTPVTAMKLLIDQEKNQERKQALLYEWSR
                     INSMLDTQLYITRLESQRKDMYFDYVSLKRMVIDEIQLTRHISQVKGIGFDVDFKVDD
                     YVYTDIKWCRMIIRQILSNALKYSENFNIEIGTELNDQHVSLYIKDYGRGISKKDMPR
                     IFERGFTSTANRNETTSSGMGLYLVNSVKDQLGIHLQVTSTVGKGTTVRLIFPLQNEI
                     VERMSEVTNLSF"
     gene            9708..10469
                     /gene="vraF"
                     /locus_tag="O0293_001472"
     CDS             9708..10469
                     /gene="vraF"
                     /locus_tag="O0293_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein VraF"
                     /protein_id="HCY0280360.1"
                     /translation="MAILEVKQLTKIYGTKKMAQEVLRDINMSIEEGEFIAIMGPSGS
                     GKTTLLNVLSSIDYISQGSITLKGKKLEKLSNKELSDIRKHDIGFIFQEYNLLHTLTV
                     KENIMLPLTVQKLDKEHMLNRYEKVAEALNILDISDKYPSELSGGQRQRTSAARAFIT
                     LPSIIFADEPTGALDSKSTQDLLKRLTRMNEAFKSTIIMVTHDPVAASYANRVVMLKD
                     GQIFTELYQGDDDKHTFFKEIIRVQSVLGGVNYDL"
     gene            10459..12348
                     /gene="vraG"
                     /locus_tag="O0293_001473"
     CDS             10459..12348
                     /gene="vraG"
                     /locus_tag="O0293_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease VraG"
                     /protein_id="HCY0280361.1"
                     /translation="MTFNEIIFKNFRQNLSHYAIYLFSLITSVVLYFSFVALKYAHKL
                     NMTESYPIIKEGSQVGSYFLFFIIIAFLLYANVLFIKRRSYELALYQTLGLSKFNIIY
                     ILMLEQLLIFIITAILGIIIGIFGSKLLLMIVFTLLGIKEKVPIIFSLRAVFETLMLI
                     GVAYFLTSAQNFILVFKQSISQMSKNNQVKETNHNKITFEEVVLGILGIVLITTGYYL
                     SLNIVQYYDSIGTLMFILLSTVIGAYLFFKSSVSLVFKMVKKFRKGVISVNDVMFSSS
                     IMYRIKKNAFSLTVMAIISAITVSVLCFAAISRASLSSEIKYTAPHDVTIKDQQKANQ
                     LASELNNQKIPHFYNYKEVIHTKLYKDNLFDVKAKEPYNVTITSDKYIPNTDLKRGQA
                     DLFVAEGSIKDLVKHKKHGKAIIGTKKHHVNIKLRKDINKIYFMTDVDLGGPTFVLND
                     KDYQEIRKYTKAKHIVSQFGFDLKHKKDALALEKAKNKVDKSIETRSEAISSISSLTG
                     ILLFVTSFLGITFLIAVCCIIYIKQIDETEDELENYSILRKLGFTQKDMARGLKFKIM
                     FNFGLPLVIALSHAYFTSLAYMKLMGTTNQIPVFIVMGLYICMYAVFAVTAYNHSKRT
                     IRHSI"
BASE COUNT         4641 a         1640 c         2301 g         4205 t
ORIGIN      
        1 cattcattta ttctgtttta tactatgact gataatacca aggaggtaca acatgatgaa
       61 aaagttaatc aataaaaaag aaacattttt aactgatatg cttgaaggat tgttaattgc
      121 gcacccagag ttagatctga ttgctaatac agttattgta aaaaaagcta agaaagaaca
      181 tggtgtagca atagtctctg gaggtggaag cggacatgaa cctgcgcatg ccggttttgt
      241 tgcagaaggt atgctagatg cagcggtttg tggcgaagta tttacatcac ctacacctga
      301 taaaatatta gaagctatta aagcagtaga tactggtgat ggtgtattac tagttgtaaa
      361 aaactatgca ggtgacgtga tgaatttcga aatggcacaa gagcttgcag aaatggaagg
      421 tataaatgtt caaactgtta ttgttcgtga cgacattgct gtgacaaacg aagtacaacg
      481 tcgtggtgtt gcaggaacag tgtttgttca taagcttgcc ggttatcttg ctgaaaaagg
      541 ttattcatta acagagataa aatcgcgtgt agaagcgttg ttacctgaaa ttaaaagtat
      601 tggtatggca attgagccac cgcttgttcc aactactgga aaatatggct ttgatattga
      661 agacgacaaa atggaaatcg gtattggtat acatggtgaa aaaggtattc atagggaaga
      721 agtaaaggat attgatcata ttgttggaac attgttagac gaattgtata aagaagttac
      781 tgccaatgat gtcatattaa tggtaaatgg tatgggtggt acgccgttat ctgaattaaa
      841 tatcgtaact aaatatattc aacaaaattt agctgcaaga acggttaatg ttgctaaatg
      901 gtttgttggt gattatatga catctttaga catgcaaggt ttttctataa ctatcgtgcc
      961 taataaacca gaatatttgg aagcattttt agcaccaaca acaagtcaat actttaaata
     1021 agaagtgaat atgaataaat atacatttat gaggtggcac aaatgaaagt gaatgatatg
     1081 aaagcacgtt tattaaattt agaagaaacg tttaaaaaac atgaatctga attaactgaa
     1141 ttagatcgag caattggtga tggtgaccac ggggttaaca tggttcgtgg gtttagtagt
     1201 cttaaagaca aacttgatga tagctcaatg caatcattgt tcaaatcaac tggtatggca
     1261 ttgatgtcaa atgttggggg tgcatcagga ccactgtatg gctttagctt tgttaaaatg
     1321 tctgcagtca ccaaagatga tatggataat caagatttca ttacactaat tcaggcattt
     1381 gccgaagcgg ttgaatcacg tggtaaagtt actttaaatg aaaagacaat gtatgatgta
     1441 gtagcgcgag cagcagagaa gcttaaaaat ggtgaaactt taacattcaa tgatttacag
     1501 caattagcag ataatacaaa agatatggta gcaacgaaag gtagagctgc atattttgga
     1561 gaagaatcaa aaggttatat tgatccaggt gctcaaagta tggtttatat tttaaacgct
     1621 ttgattggag atgaagataa tgcctaaaat tatacttgtt agccacagta aagaaattgc
     1681 aagtggtaca aaatctttgt taaagcaaat ggcaggtgac gttgatatta taccaatcgg
     1741 gggattacca gatggttcaa ttggaacttc atttgatatc atccaagaag ttttgactaa
     1801 attagaggat gatgcattgt gtttttacga tattggatct tcagaaatga atgtagatat
     1861 ggcaattgaa atgtatgatg gtaatcatcg tgtgttaaaa gttgatgcac caattgttga
     1921 aggcagtttt atcgcagcag taaagctatc aatcggcggt tcaattgatg atgcattagc
     1981 agaaatcaaa caatcatttt agttaaaatt tactaataat gaaaaatgta aacctttttc
     2041 aaatgaaact ttataaaaaa tatgatagta tatatgtaaa tgtttaataa aatctggaga
     2101 aataggagga cattgccatg caacacctta taaaaaaaca tgtattgaat ggcgagtttg
     2161 atttagtacg acaattgatg tccgaaacag attttatgga atttgaagaa gcatatattt
     2221 caagtgcgca tgaagtagaa agtatgatgt tttatacatg tattttagat atgattaagt
     2281 acgaagaatc atctgaaatg catgacttag catttttatt gcttgtgtat ccactaagtg
     2341 aatatgaagg tgctttggat tctgcttatt atcatgcaga cgcttccata aaacttactg
     2401 acggcaaaga agttaaaagt ttgttacaaa tgttattatt gcatgcgata ccaacacctg
     2461 ttatttcaga taagaaggct tttgatatcg ccaagcaaat tttaaaatta gatcctaata
     2521 ataatgttgc tcgtaacgtc ttaaaagaca ctgccaaacg tatggacaac gttgttgttg
     2581 atataaatga attacaccaa cgtaatgcac gttaattaca tttcaattat attagcttaa
     2641 taatagtttt aacatttggt tgggttgggc atatgttcca gcctttttta atacttaaaa
     2701 actaacgaag tatacttgtg tgcacaaatg gtttttatac aacattttat aaatttatac
     2761 attttaataa agaacatacg atagatggtt taaaccttgt taactgagaa attttgatat
     2821 gtattcttcg aaatttaact aaatatacga aattcaagaa gcacaataat taatcatttt
     2881 tcctatacaa aagttcgtat gactgcatta taaaagcata aatttataat ttttttaaat
     2941 gtcattgaac gtgataatgt gaatggattg agcaattttg aaaaagtgaa aaataaccta
     3001 tgcggcttgc aattaatttt cagtacgtta taatgcacac tgtgcaaaat taaggaggtc
     3061 tattattcac atgatgatga ataaagaagc aacaaaaatt ggatttgcct acgtcggcat
     3121 tgtagtgggc gcaggatttt caactggaca agaagttatg caatttttca ctaaatatgg
     3181 cttgtgggct tatttaggtg ttattatatc tggttttatt ttagctttta ttgggcgcca
     3241 agtagcaaaa attggtactg cctttgaagc gacaaatcat gaatcaacat tacaatacgt
     3301 attcggtgaa aagtttagta aagtctttga ttatatttta atcttcttct tatttggtat
     3361 agctgtaacc atgatagctg gtgcaggcgc aacatttgaa gaaagttata acatacctac
     3421 atggctaggt gctttaatta tgacattagc gatttatatt acgttgctat tagactttaa
     3481 taaaatagta cgtgcactag gtatcgttac accattttta attgttttag ttgtattaat
     3541 cgctggcgtt tatttattta aaggtcatgt ttcattagca gaagttaacc aagtagtgcc
     3601 tgaagcaagt atttggaagg gaatctggtt tggtacaata tatggtggat tagctttttc
     3661 tgtaggtttt agtaccatcg tagcaatcgg tggggatact gaaaagcgta cagtgtcagg
     3721 tgcaggcgcg atgtatggtg gtattatcta tactgtatta ctagcattga tcaactttgc
     3781 attgcaaagt gaatatccaa ctattaaaaa tgcctcaatt cctacattga cgttagcaaa
     3841 taatatccat cctttaatag caacagtgtt atctgttatt atgctggcgg ttatgtataa
     3901 tactattcta ggactaatgt attcatttgc agcacgtttt acagaaccat acagtaaaaa
     3961 ttatcatatc tttattatta taatgatggt agcaggttat ttattaagtt tcgtaggatt
     4021 tgctgaatta attaataagt tatatacaat tatgggatat gtaggcttat ttattgtagt
     4081 agctgtaatt attaaatatt tcaaacgtaa aaatgcggat aaaaaacata ttgcttaata
     4141 tcatatgagg gatatccgaa actttacaat tgaatcactt tgttttaacc ttaaaagcaa
     4201 ttcgtctcta ctcttatcgg gcgaattgct ttttatattt attcagtcta ttaatatgag
     4261 cgtctaacaa atagagaggt acgatgtaat gaataaagat aataaatgga cgatgataac
     4321 tgcgcttttt ataactgtaa tcagtgtatt gttagcattt catctgaaac aacattatga
     4381 ccaaattaca aatgagaacc atgctaataa agacaaaatt aatattaaaa ataaaaatgt
     4441 gcgcatttat caaaacctta catacaatag agttttccct aacagtaaat tagatattat
     4501 tacacctgtt gatatgtctt ctaatgccaa actgccagtt attttttgga tgcacggtgg
     4561 tggttatatt gcgggtgata agcagtataa aaacccatta ttagcgaaaa ttgctgaaca
     4621 agggtacatt gttgtgaatg taaattatgc attggcgcca caatataaat atcccacacc
     4681 attaattcaa atgaatcaag caactcaatt cattaaagaa aataaaatga atttacctat
     4741 tgattttaat caagtaatta ttggcggtga ttctgcaggt gctcaattag ctagccaatt
     4801 tacggcaata cagacgaatg atcgcttaag agaagccatg aaatttgatc agtcattcaa
     4861 accatcgcaa attaaaggtg ctatactatt tggtggtttt tataatatgc aaacagttag
     4921 agaaactgag tttccaagaa tacagttatt tatgaaaagt tatactggcg aagaagattg
     4981 ggaaaagagt tttaaaaaca tttcacaaat gtcgacagta aaacaatcga caaaaaatta
     5041 tccaccaaca tttttatctg ttggagatag cgatccattc gaaagtcaaa atatagaatt
     5101 cagtaagaaa ttacaagaat tgaatgtacc agtagatact ttgttttatg atggtacgca
     5161 tcatttacat catcagtatc aatttcacct taataaacct gaatcgatag ataatatcaa
     5221 aaaagtgtta cttttcttaa gtcgtaatac atcctctagt ggtattcaaa ctgaagagaa
     5281 accacaaata gaaaatccga gtaatgaatt accgttaaat cctttaaact aatgataaac
     5341 agtagtaatt tattgcttaa gcaacattta agattttcaa attaaaaacg aagaatttaa
     5401 aacatgtggt gctaatgtgt aagagtctga gatataataa attgttagcg gttctttatc
     5461 atttctatct cactctatta tgatgtgaca tttattttac aaactaattt gttttgaacc
     5521 tgaaaataac tttttacaac taaattgtcg aaaaaacagt gtgtacatga tataataaat
     5581 ttataaattg aaaagaattc aaaagaaaaa ttaaaatata gattgagcac gaagtgattt
     5641 gaaataaggt tgtgaaaggg aatgacaagg tcagcattaa aaccatttaa aaataaacgc
     5701 gttatggtta ctggacgtat acaacgtgtt ttgtttaaaa attatttaga tagacatagc
     5761 acatttaagc cgaatgtaag gatattatta aaagatgtat ttgtttcagg tgtatcaata
     5821 gatcatttat ggttatatga gacaaataaa tactatgcat tggcaatgga acttattcat
     5881 caacgagtaa aatttagtgc gaatgttgta ccgtattaca aaataaatag aaataataat
     5941 ttattcgtac aagattatgg aattaagcgt aaaggtaggt taattactga agaagcttac
     6001 aatcaaaaca atcagtatca ggataagata tatgaaaaat taccggatat agattttaga
     6061 ctcgaagatt tttatagtaa ggaaaactaa acaacatata taaagcacca ctaatcgtag
     6121 ttgatagtta atcaattaca cattagtggt gttttatttt gaatttaaat ttgataaatg
     6181 aataagagtg tacgtattta tttgagatta gataacattg ataagtatga gtaatgtatt
     6241 aggtaaccat taaattcgtt gttatacgat tctgatacaa gattatgata aaatagcttt
     6301 aattaagaat tttagccatc atatagtcat cataatattt accatcgata aataacttat
     6361 cttttaaaac gccttcgatt tgaaaatcgg cacttttaaa aagctcgagg gcaggttggt
     6421 tattgagtgg tacatttgct tcaattcggt gtatttgatt gtttaaacac caagccataa
     6481 tggcatcaag aagtgcttgg ccaattccac gatgttgata taatttcttt acacctaaat
     6541 caattttagc aacatgttta atgcgttgaa atggtgtcgt attaacaaag gcaaagccaa
     6601 cgagttgttc atcactttca gcaacgaaga tgactttatg cggagaagtg atatattctt
     6661 ctaattgttt actagccgat gtgacgctag gatcatattc tcctggtgtg tagaacatat
     6721 acggagattc gtcgtatatg ttcgctaaca ttgaaatgaa attttctaca tctttgatac
     6781 taactctacg tataatatgg gccataaaaa gcctccagta ttttgaaatt ttaaaacatt
     6841 tgctactatt ataatatata tgtaactaaa aggtggagta atatgttttt tggttacagt
     6901 ttaaaggagt attttagatg aaacctaaag ttttattagc aggtggaaca ggatatattg
     6961 gtaagtattt aagtgaagtg attgaaaatg atgctgaact ttttgctata tcaaaatatc
     7021 cagacaataa aaaaacagat gatgttgaaa tgacttggat tcagtgtgat atatttcatt
     7081 acgaacaggt tgttgcagca atgaatcaaa tagatattgc tgtattcttt atcgacccaa
     7141 caaagaattc tgccaaaata acacaatcat cagcaagaga tttaacatta atcgcagcag
     7201 ataattttgg tcgagcagcg gctatcaatc aagtaaaaaa agtaatctac atacctggga
     7261 gtcgttatga taatgaaaca attgaacgcc taggtgcata tggtacacct gtagaaacaa
     7321 caaatttagt ttttaaacgt tctttagtta atgtagaatt acaagtttca aagtatgatg
     7381 atgttagatc aacgatgaag gtagttttac caaagggatg gacattaaag aacgttgtaa
     7441 accattttat tgcatggatg ggttacacta aaggaacttt tgtgaaaaca gaaaaatcac
     7501 atgatcaatt taagatatat attaagaata aggtgcgacc gctcgcagta tttaaaatag
     7561 aagaaacagc tgatggaata ataactttaa ttttattgag tggaagttta gtgaaaaaat
     7621 atacagttaa tcaagggaag ttagaattta gattaatcaa agagtcggca gtcgtttata
     7681 tacatctata cgattatatc cctcgattat tttggccgat ttattacttt atacaagcac
     7741 caatgcaaaa aatgatgatt catggctttg aagttgactg ccggattaaa gattttcaaa
     7801 gtcgattaaa atcaggagaa aatatgaaat atactaaatg atattgggtg atatggatgc
     7861 aaatactact agtagaagat gacaatactt tgtttcaaga attgaaaaaa gaattagaac
     7921 aatgggattt taatgttgct ggtattgaag atttcggcaa agtaatggat acatttgaaa
     7981 gttttaatcc tgaaattgtt atattggatg ttcaattacc taaatatgat gggttttatt
     8041 ggtgcagaaa aatgagagaa gtttccaacg taccaatatt atttttatca tctcgtgata
     8101 atccaatgga tcaagtgatg agtatggaac ttggcgcaga tgattatatg caaaaaccgt
     8161 tctataccaa tgtattaatt gctaaattac aagcgattta tcgtcgtgtc tatgagttta
     8221 cagctgaaga aaaacgtaca ttgacttggc aagatgctgt cgttgatcta tcaaaagata
     8281 gtatacaaaa aggtgatcag acgattttcc tgtccaaaac agaaatgatt atattagaaa
     8341 ttcttattac caaaaaaaat caaatcgttt cgagagatac aattatcact gcattatggg
     8401 atgatgaagc atttgttagt gataatacgt taacagtaaa tgtgaatcgt ttacgaaaaa
     8461 aattatctga aattagtatg gatagtgcaa tcgaaacaaa agtaggaaaa ggatatatgg
     8521 ctcatgaata atttgaaatg ggtagcttat tttttgaaat ctcgcatgaa ctggatattt
     8581 tggatattgt ttttaaactt ccttatgtta ggcattagtc taatcgatta tgattttcca
     8641 atagacagtt tattttatat tgtttctttg aatttaagtt taacaatgat ttttctttta
     8701 ttgacatatt ttaaagaagt aaaattatat aagcattttg acaaagataa agaaatagaa
     8761 gaaattaaac ataaagattt agcggaaacg ccatttcaac gtcatacagt tgattattta
     8821 tatcgtcaaa tctcagcgca caaagaaaag gttgttgagc aacagttgca attgaacatg
     8881 catgaacaaa ccattacaga atttgtgcac gacataaaaa cacctgtgac agctatgaaa
     8941 ttattaattg atcaagaaaa aaatcaagaa agaaaacaag cattactata tgaatggtct
     9001 cgtataaact cgatgctaga tacacagctg tatattacta gattagaatc tcaacgtaaa
     9061 gatatgtatt ttgattacgt gtcacttaaa cgcatggtca ttgatgaaat acaattaaca
     9121 agacatatta gtcaggttaa aggtattggt tttgatgttg actttaaagt ggatgattat
     9181 gtttatacag atataaaatg gtgtcgtatg attattagac aaattttgtc aaacgcattg
     9241 aaatatagtg agaattttaa tattgaaatt gggacagaat taaatgatca acatgtttcg
     9301 ttatatatta aagactatgg cagaggtatt agtaaaaaag atatgccgcg aatatttgaa
     9361 cgaggattta cgtcaacggc taacagaaat gaaacgacgt cttcaggtat gggtctatat
     9421 ttagtaaata gtgtaaagga tcaattaggt attcacctgc aagtcacgtc gactgttggt
     9481 aaggggacaa ctgtcagatt gattttccca ttacaaaatg aaattgttga acgcatgtcg
     9541 gaagtgacaa atttgtcatt ttaaacatgc gttttgttac ttagaattga tacatcaatg
     9601 cagcttcaac gttataataa gatagatgtt agtcatatgt taaattgaag atacaagtgc
     9661 caaagcctaa aggaaatgaa gttaagataa attataggag tgttaaagtg gcaattttag
     9721 aagtaaaaca attaacaaaa atatatggaa ctaaaaaaat ggcacaagaa gtgttgcgag
     9781 atatcaatat gtctattgaa gaaggcgagt ttattgctat tatgggtccc tctggatctg
     9841 ggaaaacgac attattaaat gttttaagtt caattgatta tatttcacaa ggttctatta
     9901 cattaaaagg aaaaaaatta gaaaagcttt caaacaagga attatctgat atacgcaagc
     9961 atgatattgg ttttattttt caagagtata atttactgca tacattgact gttaaagaaa
    10021 acataatgtt accactaacg gtacagaagt tagataaaga acatatgtta aatcgttatg
    10081 aaaaagtagc agaagcatta aatatattgg atattagtga taaatatccc tctgaattgt
    10141 ctggtggaca aaggcaacga acatcagctg ccagagcatt tataacattg ccttctatta
    10201 tatttgctga cgaaccaaca ggtgcattgg attctaaaag tactcaagat ttattaaaac
    10261 gattaacaag aatgaatgaa gcatttaagt ctacaattat tatggtaacg catgatcctg
    10321 ttgcagcaag ctatgcaaat cgagtagtga tgctaaaaga tggtcaaatt ttcactgaat
    10381 tataccaagg ggatgacgat aaacatacct ttttcaaaga aataatacgt gtacaaagtg
    10441 ttttaggtgg cgttaattat gacctttaac gagataatat ttaaaaattt ccgtcaaaat
    10501 ttatcacatt atgccatcta tcttttttcg ttaattacga gtgtagtatt gtattttagc
    10561 tttgtagcat taaaatacgc tcataaacta aacatgacag agtcatatcc aattataaag
    10621 gaaggctcac aagtcggaag ctactttcta tttttcatca taattgcatt tttgttatat
    10681 gccaatgtgt tatttattaa acgacgaagt tatgagcttg cattatatca aacattaggt
    10741 ttatctaaat tcaacattat ttatatacta atgctcgaac aattactaat atttataatt
    10801 acggcaatat taggtattat tattggtatt tttggttcga aactgttatt aatgattgtc
    10861 tttacattat taggaattaa agaaaaggtt ccaattattt ttagtttgag ggcggtattt
    10921 gaaacattaa tgttaatcgg tgtcgcttat tttttaacat ctgctcaaaa ttttatatta
    10981 gtgttcaaac aatctatttc acagatgtca aagaataacc aggttaaaga aacaaatcat
    11041 aataaaatta catttgaaga ggttgtttta ggcatcttag gtatagtatt gattaccaca
    11101 ggatactatc tatctttgaa cattgttcaa tattatgatt ctatcggtac acttatgttt
    11161 attttattgt caactgtgat tggggcatac ttatttttta aaagctctgt ttctctagtt
    11221 tttaaaatgg tgaagaagtt tagaaaaggt gttataagtg taaatgatgt catgttctca
    11281 tcatctatta tgtatcgtat taagaaaaat gctttttcac ttacggtcat ggcaatcatt
    11341 tcagcgatta ctgtttcagt tctttgcttt gctgctataa gtagagcgtc cttatcaagt
    11401 gaaataaaat atactgcacc acacgacgtt acaattaaag accaacaaaa agctaatcaa
    11461 ttagcaagtg aattaaacaa tcaaaaaatt cctcattttt ataattataa agaagtaatt
    11521 catacgaaat tgtataaaga taatttattt gatgtaaaag cgaaagaacc atacaatgta
    11581 acaattacta gtgataaata catccctaat actgatttga aacgtgggca agctgattta
    11641 tttgtagcgg aaggttctat caaagattta gtgaaacata agaagcatgg taaggcaatt
    11701 ataggaacga aaaaacatca tgttaatatt aagttacgta aagatattaa taaaatctat
    11761 tttatgacag atgttgattt aggtggacca acgtttgtct taaatgacaa agactatcaa
    11821 gaaataagaa agtatacaaa ggcaaagcat atcgtctctc aatttggatt cgatttgaaa
    11881 cataaaaaag atgctttagc attagaaaaa gcgaaaaata aagttgataa atctattgaa
    11941 acaagaagtg aagcgataag ctcaatatca agtttaaccg gaatattatt atttgtaaca
    12001 tcatttttag gtattacatt cttgattgct gtatgttgca ttatatacat aaagcaaata
    12061 gatgaaaccg aagatgagtt agagaattat agtattttga gaaagcttgg atttacacaa
    12121 aaagatatgg caaggggact aaagtttaaa attatgttta attttgggtt acctttagtt
    12181 attgcactat cacatgcata ttttacatca ttagcatata tgaaattaat gggtacaacg
    12241 aatcaaatac cggttttcat agtaatggga ttatacattt gtatgtatgc tgtttttgca
    12301 gtgacggctt ataatcattc caagcgaaca attagacatt ccatataaaa tatacagatg
    12361 gctttcagta gagtagtgga ttcggattca cgaactatac tggaagcttt ttattataaa
    12421 tgaagagaag ttatattttt agcatgtata gttgaatact gggttaaaat accatattaa
    12481 taatgaagta aaggtatgag tgattatgaa agtgttttga atgaaatata tttaattggt
    12541 gatgctttta attgaaaaga ttaacaggat tcaactttgt aaattgtatt aaatgtgaga
    12601 aaataaaagt atattcattg agagatatat gagtcaatga tcgttttaaa caagataagt
    12661 gtattttaat atgtaaaagt tatgtaataa atattgtatc gttgcaaatt tcccaactat
    12721 atccatttac aatttttaga gttgtataga taaaaaattg cctatacaat aactttttca
    12781 aaacaaa
//
LOCUS       DAKTIO010000071        12784 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.071, whole genome shotgun sequence.
ACCESSION   DAKTIO010000071 DAKTIO010000000
VERSION     DAKTIO010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12784
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.071"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..138
                     /locus_tag="O0293_001474"
     CDS             <1..138
                     /locus_tag="O0293_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCY0280362.1"
                     /translation="ALGQVRHYQLDELLNIIDACAETDYKLKSSYMDKQLILELFILS
                     L"
     gene            complement(183..434)
                     /locus_tag="O0293_001475"
     CDS             complement(183..434)
                     /locus_tag="O0293_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCY0280363.1"
                     /translation="MANIKSAIKRVKTTEKAEARNISQKSAMRTAVKNAKTAVSNNAD
                     NKNELVSLAVKLVDKAAQSNLIHSNKADRIKSQLMTANK"
     gene            780..2603
                     /gene="lepA"
                     /locus_tag="O0293_001476"
     CDS             780..2603
                     /gene="lepA"
                     /locus_tag="O0293_001476"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCY0280364.1"
                     /translation="MDNEQRLKRRENIRNFSIIAHIDHGKSTLADRILENTKSVETRD
                     MQDQLLDSMDLERERGITIKLNAVRLKYEAKDGNTYTFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNELELLPVINKIDLPAAEPERVKQEIEDMI
                     GLDQDDVVLASAKSNIGIEEILEKIVEVVPAPDGDPEAPLKALIFDSEYDPYRGVISS
                     IRIVDGVVKAGDKIRMMATGKEFEVTEVGINTPKQLPVDELTVGDVGYIIASIKNVDD
                     SRVGDTITLASRPASEPLQGYKKMNPMVYCGLFPIDNKNYNDLREALEKLQLNDASLE
                     FEPESSQALGFGYRTGFLGMLHMEIIQERIEREFGIELIATAPSVIYQCVLRDGSEVT
                     VDNPAQMPDRDKIDKIFEPYVRATMMVPNDYVGAVMELCQRKRGQFINMDYLDDIRVN
                     IVYELPLAEVVFDFFDQLKSNTKGYASFDYEFIENKESNLVKMDILLNGDKVDALSFI
                     VHRDFAYERGKALVEKLKTLIPRQQFEVPVQAAIGQKIVARTNIKSMGKNVLAKCYGG
                     DISRKRKLLEKQKAGKAKMKAVGNVEIPQDAFLAVLKMDDE"
     gene            3166..4290
                     /locus_tag="O0293_001477"
     CDS             3166..4290
                     /locus_tag="O0293_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCY0280365.1"
                     /translation="MTVQSAYIHIPFCVRICTYCDFNKYFIQNQPVDEYLDALITEMS
                     TAKYRILKTMYVGGGTPTALSINQLERLLKAIRDTFTITGEYTFEANPDELTKEKVQL
                     LEKYGVKRISMGVQTFKPELLSVLGRTHNTEDIYTSVLNAKNTGIKSISLDLMYHLPK
                     QTIEDFEQSLDLALDMDIQHISSYGLILEPKTQFYNMYRKGLLKLPNEDLGADMYQLL
                     MSKIEQSPFHQYEISNFALDGHESEHNKVYWFNEEYYGFGAGASGYVDGVRYTNINPV
                     NHYIKAINKESKAILVSNKPSLTERMEEEMFLGLRLNEGVSSSRFKKKFDQSIESVFG
                     QTINNLKEKELIVEKNDVIALTNRGKVIGNEVFEAFLIND"
     gene            4390..5367
                     /gene="hrcA"
                     /locus_tag="O0293_001478"
     CDS             4390..5367
                     /gene="hrcA"
                     /locus_tag="O0293_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HCY0280366.1"
                     /translation="MITDRQLSILNAIVEDYVDFGQPVGSKTLIERHNLNVSPATIRN
                     EMKQLEDLNYIEKTHSSSGRSPSQLGFRYYVNRLLEQTSHQKTNKLRRLNQLLVENQY
                     DVSSALTYFADELSNISQYTTLVVHPNHKQDIINNVHLIRANPNLVIMVIVFSSGHVE
                     HVHLASDIPFNNDKLNTISNFVTNKLTEFNQNLQDDIVSFVQSEQEEIFINKLLNTMN
                     NHISNQSNSIYMGGKVKLIDALNESNVSSIQPILQYIESNRIAELLQDISSPNINVKI
                     GNEIDDSLSDISIVTSQYHFDETLKGQIAVIGPTAMHYQNVIQLLNRIW"
     gene            5399..6025
                     /gene="grpE"
                     /locus_tag="O0293_001479"
     CDS             5399..6025
                     /gene="grpE"
                     /locus_tag="O0293_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCY0280367.1"
                     /translation="MTNKDESVEKNTESTVEETNVKQNIDDSVEQAEESKGHLQDEAI
                     EETSDENVIEEIDPKDQKINELQQLADENEEKYLRLYAEFENYKRRIQKENEINKTYQ
                     AQRVLTDILPAIDNIERALQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEG
                     EAFDPNIHQAVVQDDNPDFESGEITQELQKGYKLKDRVLRPSMVKVNQ"
     gene            6094..7926
                     /gene="dnaK"
                     /locus_tag="O0293_001480"
     CDS             6094..7926
                     /gene="dnaK"
                     /locus_tag="O0293_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCY0280368.1"
                     /translation="MSKIIGIDLGTTNSCVTVLEGDEPKVIQNPEGSRTTPSVVAFKN
                     GETQVGEVAKRQAITNPNTVQSIKRHMGTDYKVDIEGKSYTPQEISAMILQNLKNTAE
                     SYLGEKVDKAVITVPAYFNDAERQATKDAGKIAGLEVERIINEPTAAALAYGLDKTDK
                     DEKVLVFDLGGGTFDVSILELGDGVFEVLSTAGDNKLGGDDFDQVIIDYLVAEFKKEN
                     GVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFE
                     ELSDSLIRRTMEPTRQAMKDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEIGKEPNKG
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSK
                     SQIYSTAVDNQPSVDVHVLQGERPMAADNKTLGRFQLTDIPPAERGKPQIEVTFDIDK
                     NGIVNVTAKDLGTNKEQRITIQSSSSLSDEEIDRMVKDAEVNAEADKKRREEVDLRNE
                     ADSLVFQVEKTLTDLGENIGEEDKKSAEEKKDALKTALEGQDIEDIKSKKEELEKVIQ
                     ELSAKVYEQAAQQQQQAQGANAGQNNDSTVEDAEFKEVKDDDKK"
     gene            8062..9201
                     /gene="dnaJ"
                     /locus_tag="O0293_001481"
     CDS             8062..9201
                     /gene="dnaJ"
                     /locus_tag="O0293_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCY0280369.1"
                     /translation="MAKRDYYEVLGISKDASKDEIKKAYRKLSKKYHPDINKEEGADE
                     KFKEISEAYEVLSDDNKRASYDQFGHDGPQGFGGQGFNGSDFGGFSGFGGGGFEDIFS
                     SFFGGGRQRDPNAPQKGDDLQYTMTLTFEEAVFGTTKEISIRKDVTCETCHGDGAKPG
                     TSKKTCSYCNGAGHVAVEQNTILGRVRTEQVCPKCNGSGQEFEEACPTCHGKGTENKT
                     VKLEVKVPEGVDNEQQIRLAGEGSPGVNGGPAGDLYVVFRVKPSETFKRDGDDIYYKL
                     NVSFPQAALGDEIKIPTLNNEVMLTIPAGTQTGKQFRLKEKGIKNVHGYGYGDLYVDI
                     KVVTPTKLTDRQKELMKEFAQLNGEEINEQPSNFKDRAKRFFKGE"
     gene            9205..10143
                     /gene="prmA"
                     /locus_tag="O0293_001482"
     CDS             9205..10143
                     /gene="prmA"
                     /locus_tag="O0293_001482"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCY0280370.1"
                     /translation="MNWTELSIIINHEAVELATNILENHGSNGVVIEDSDDLINQPED
                     KYGEIYALKKEDYPDKGVRLKAYFNEMTYDDKLRQQIKDELLNLDELDQHNVQFSEQI
                     IAETDWENEWKNYFHPFRASKKFTIVPSWETYAKEADEELCIELDPGMAFGTGDHPTT
                     SMCLKAIETYVLPQHSVIDVGTGSGILSIASHLIGVKRIKALDIDEMAVSVAKENFRR
                     NHCETLIEAVPGNLLKDETEKFDIVIANILAHIIDEMIEDAYNTLNEGGYFITSGIIK
                     EKYEGIQSHMERVGFKIISEQHDNGWVCLVGQKVSE"
     gene            10145..10897
                     /locus_tag="O0293_001483"
     CDS             10145..10897
                     /locus_tag="O0293_001483"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCY0280371.1"
                     /translation="MQRYFIDQNADVSQRFFITKKEDIHHITNVMRYDVGSKIILTFN
                     DQTVYLCEIADITSDKIEVDLKEKQNINTELPVDVTICSGLIKADKYEWLLQKATELG
                     ASSFIAVSMERSIVKLNEAKVAKKIERWQKIIKEAAEQSYRLVIPSIQFESNLKLICD
                     TIDNYDYILIAYEEEAKDGELSNFKQTLQQFNAQDKVLMIFGPEGGLSENEISLFSNT
                     STVVGLGPRILRAETAPLYALSAISYEKELMG"
     gene            10904..12250
                     /gene="mtaB"
                     /locus_tag="O0293_001484"
     CDS             10904..12250
                     /gene="mtaB"
                     /locus_tag="O0293_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HCY0280372.1"
                     /translation="MSTVAFHTLGCKVNHYETEAIWQLFKEANYERVDFEANADVFVI
                     NTCTVTNTGDKKSRQIIRRAIRQNPDAVICVTGCYAQTSSAEIMEIPGVDVVVGTQDR
                     HKLLGYIDEFRKERQPINGVGNIMKNRKYEELDVPYFTDRTRASLKIQEGCNNFCTFC
                     IIPWARGLMRSRDPEKVVEQATQLVNSGYKEIVLTGIHTGGYGQDLKDYNLAQLLRDL
                     ETINGLERIRISSIEASQLTDEVIDVLERSTKVVRHLHIPLQSGSDTVLKRMRRKYTM
                     DRFSERLTKLHKALPDLAVTSDVIVGFPGETEAEFQETYDFIVKHKFSELHVFPYSPR
                     IGTPAARMDDQIDEEIKNERVHKLITLSNQLGKLYASKFDQDVLEVIPEEQGDTEGTL
                     VGYADNYMKVQFEGDESLIGQIVKVKITQANYPLNEGQAIKVVDFATNKSDREVLV"
     gene            12543..12719
                     /locus_tag="O0293_001485"
     CDS             12543..12719
                     /locus_tag="O0293_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCY0280373.1"
                     /translation="MSKTVVRKNESLEDALRRFKRSVSKSGTIQEVRKREFYEKPSVK
                     RKKKSEAARKRKFK"
BASE COUNT         4788 a         1751 c         2453 g         3792 t
ORIGIN      
        1 gcgttaggac aagtaagaca ttatcaactt gatgaattat tgaatattat agatgcttgt
       61 gcggaaactg attataaact taaatcatca tatatggata aacagttaat actggaatta
      121 ttcattctat ctttataaaa aataaaagtt caagcatgcg cttgaacttt tatttaaaaa
      181 gattatttat ttgcagtcat taattgtgat ttaatacggt cagctttgtt tgaatgtatt
      241 aaattacttt gagcagcttt gtctactaac ttaactgcta agcttactaa ttcattttta
      301 ttatcagcgt tatttgaaac agctgtttta gcgtttttaa ctgctgtacg cattgcactc
      361 ttttgtgaaa tgttgcgtgc ttcagctttt tcagttgttt ttacacgttt aattgcagat
      421 ttgatatttg ccatttctgt cacctcctaa aagtgagttc atcttatcaa aattttattt
      481 gattacaaca agaaatattt taacaaaagt ctaaccgttg tgcaatcatt tatttgatgg
      541 ggttagctta aaattttgca gatttaataa attgtgttgg aaacacaatc aatacgtttt
      601 ataattataa gtacgtaatt aacatattaa catatcgcaa gtatgtattt aaataaagat
      661 tgttataatt tcaaagttca tccaagatta tggcgtttgc atttacctat taaaaacgtt
      721 attatatcaa agatgcgaaa gataatacgg gtttatttta tgaaagtgag aaggataaaa
      781 tggataatga gcaacgctta aaaagaagag agaatataag gaatttctcg attatagcac
      841 atattgacca cggaaaatct acattggctg atagaatttt agaaaatacc aaatcagttg
      901 aaacaagaga tatgcaagat cagttactag attcaatgga tttagaaaga gaacgtggta
      961 ttacaatcaa attaaacgca gtacgtttaa agtacgaagc taaagatgga aatacttata
     1021 cattccattt aatcgatacg cctggacacg tcgattttac atatgaagtg tcacgttctt
     1081 tggcagcttg tgagggcgcg attttagtag tagatgcggc tcaaggtatc gaagcacaaa
     1141 cattagcaaa tgtttattta gcattagata atgagttaga gttattgcct gttattaaca
     1201 aaattgattt acctgctgca gaacctgaac gcgtgaaaca agaaattgaa gatatgatag
     1261 gtttagacca agacgatgtt gttttagcaa gtgctaaatc taacattgga attgaagaga
     1321 tactagagaa aatagttgaa gttgtgccag ctccagatgg tgacccagaa gcaccactaa
     1381 aagcgttaat atttgattct gagtatgatc catatagagg ggtaatttca tcgataagaa
     1441 ttgtggacgg tgttgttaaa gccggagata aaattcgaat gatggccact ggtaaagagt
     1501 tcgaagtaac agaagttgga attaatacac ctaagcagct tccagttgat gaattaacag
     1561 ttggtgatgt tggttatatt attgcaagta ttaaaaatgt tgatgattct agggttggtg
     1621 acaccatcac attagctagt agacctgcat cagaaccatt gcaaggttat aagaaaatga
     1681 atccaatggt atattgcgga ctgttcccaa tagataacaa aaattataat gatttaagag
     1741 aagcattaga aaaattacaa ttgaatgatg catcattaga atttgagcct gaatcgtcac
     1801 aagcattagg ttttggttat agaactggtt tcttaggtat gttacacatg gaaataattc
     1861 aagaaagaat tgaaagagaa tttggtattg aattaattgc aactgcacca tctgtaattt
     1921 atcaatgtgt tttaagggac ggttcagaag tgacggttga taacccagca caaatgccag
     1981 atcgtgataa aattgataaa atatttgagc catatgttcg tgcaactatg atggttccaa
     2041 atgactatgt cggtgcagta atggaattat gtcaacgtaa acgtggacaa tttataaata
     2101 tggactattt agatgatatt cgtgtaaata ttgtttatga attaccttta gctgaagttg
     2161 tatttgattt cttcgatcaa cttaaatcta atactaaagg atatgcatca tttgattatg
     2221 aattcatcga aaataaagaa agtaatttag tcaagatgga tattttatta aatggtgata
     2281 aagtggatgc gctaagcttc atagttcata gagattttgc atatgaacgt ggtaaagcat
     2341 tagttgaaaa acttaaaacg ttaattccaa gacagcaatt tgaagtacct gtacaggctg
     2401 caataggaca aaaaattgta gcgcgtacaa atattaaatc aatgggtaaa aacgttttag
     2461 ctaaatgtta tggcggtgac ataagccgta aacgtaaatt acttgaaaaa caaaaagcag
     2521 gtaaagctaa gatgaaagca gttggtaatg ttgaaattcc acaagatgct ttcttggctg
     2581 tattgaaaat ggatgatgaa taattttaaa aaatcaatta acaatttaca atgaataaag
     2641 tttaataact aaaaagaggg agcctaggat aaattaacgt cctgggcttt acaatgttat
     2701 attggcagcc atcgacagag ttaaaatgag cttataacaa tggggcccca acacagaagc
     2761 tgacgaaaag tcagcttact ataatgtgca agttggggtg gggccccaac atagagaatt
     2821 tcgaaaagaa attctacagg caatgcaagt tggggtggga cgacgaaata aattttgcga
     2881 aaatatcatt tctgtcccac tcccttatgc atgagtttta ctcatgtaat tttattttta
     2941 aggacatatt acatctggct aatgtgtaag agccactaca taataaatca ttagtggttc
     3001 tttattattt ctatctcact ccctctaaac aagaataaat attaaaatga atcgatatat
     3061 tagacaatca ttgattaaac gttaaagtta aaagtaagaa taattgcaga tagtccaaca
     3121 ggatatagcc gattggataa aaagtctgag aagcggggca ttaaaatgac ggtacaaagt
     3181 gcatatatac atattccatt ttgtgtaaga atatgtacat attgtgattt caataaatat
     3241 tttatacaga atcaacctgt agatgagtac ttagatgcac taatcacaga aatgtctaca
     3301 gcaaaatata ggatcttaaa gaccatgtat gtaggtggcg gcacaccaac ggccctttct
     3361 attaatcagt tggaaagatt acttaaagca atacgtgata cgtttacaat cacaggcgag
     3421 tatacatttg aagcaaatcc tgatgagtta actaaagaga aagtccaact attagagaaa
     3481 tatggagtaa aaaggatttc aatgggcgtt caaacattca agccggagtt attgtctgtt
     3541 ttaggtagaa cgcacaatac tgaagatatt tacacttcgg tgttaaatgc taaaaacaca
     3601 ggtattaaat caatcagttt agatttaatg tatcatttac cgaaacagac gattgaagat
     3661 tttgaacaaa gtttagatct agctttagat atggatattc aacatatttc gagttacggc
     3721 ttaatacttg aacctaaaac ccaattttat aatatgtata gaaaaggctt gctcaaactt
     3781 cctaatgagg atttaggtgc tgacatgtat cagttgctga tgtctaagat agaacaatct
     3841 cctttccatc aatacgaaat atctaatttt gcattagatg gccatgaatc agaacataat
     3901 aaggtttact ggtttaatga ggaatattat ggatttggag caggtgcaag tggttatgta
     3961 gatggtgtgc gttatacgaa tatcaatcca gtgaatcatt atatcaaagc tataaataaa
     4021 gaaagtaaag caattttagt atcaaataaa ccttctttga ctgagagaat ggaagaagaa
     4081 atgtttcttg ggttgcgttt aaatgaaggt gtgagtagta gtaggttcaa aaagaagttt
     4141 gaccaatcta ttgaaagtgt ctttggtcaa acaataaata atttaaaaga gaaggaatta
     4201 attgtagaaa agaacgatgt gattgcactt acaaatagag ggaaagtcat aggtaatgag
     4261 gtttttgaag ctttcctaat aaatgattaa aaaaattgaa atttcgagtc tttaacattg
     4321 acttactttg accaatttga taaattataa ttagcacttg agataagtga gtgctaatga
     4381 ggtgaaaaca tgattacaga taggcaattg agtatattaa acgcaattgt tgaggattat
     4441 gttgattttg gacaacccgt tggttctaaa acactaattg agcgacataa cttgaatgtt
     4501 agtcctgcta caattagaaa tgagatgaaa cagcttgaag atttaaacta tatcgagaag
     4561 acacatagtt cttcagggcg ttcgccatca caattaggtt ttaggtatta tgtcaatcgt
     4621 ttacttgaac aaacatctca tcaaaaaaca aataaattaa gacgattaaa tcaattgtta
     4681 gttgagaatc aatatgatgt atcatcagca ttgacatatt ttgcagatga attatcaaat
     4741 atatctcaat atacaacttt agttgttcat cctaatcata aacaagatat tatcaataat
     4801 gtacacttga ttcgtgctaa tcctaattta gttataatgg ttatcgtatt ttcatcaggt
     4861 catgttgagc atgtacatct tgcttcagac ataccgttta ataatgacaa actaaataca
     4921 atatcaaatt ttgttacaaa caaactaact gagtttaatc agaatttaca agatgatatt
     4981 gtctcttttg ttcaatcaga gcaggaagaa atttttatca ataaattgtt aaatacgatg
     5041 aataatcata tttctaatca aagtaatagc atttatatgg gtggaaaggt taaacttatt
     5101 gatgcattga atgaaagtaa tgtatcttca attcagccca ttctacaata tatagaatca
     5161 aatagaattg cagaattatt acaagatata tcttcaccaa atattaatgt aaaaattggt
     5221 aatgaaattg atgacagctt aagtgatatt tcaattgtaa caagtcaata tcattttgat
     5281 gaaacgttaa aaggtcaaat cgctgtgatt ggacctacag ctatgcatta tcaaaatgtc
     5341 attcaattat taaatagaat ttggtaatga tggattagaa agtattggag gacagaaaat
     5401 gacaaataaa gacgaatcag ttgaaaaaaa cactgaatca acagttgaag aaacaaacgt
     5461 caaacaaaat attgatgatt cagttgaaca agctgaagaa agtaaaggtc atttacaaga
     5521 tgaagcaata gaagaaacgt ctgacgagaa tgttattgaa gaaatagatc caaaagatca
     5581 aaaaattaat gaacttcaac aattagcaga tgaaaacgaa gagaaatatt taaggctcta
     5641 cgctgagttt gaaaattata agcgtagaat tcaaaaagaa aatgaaataa acaaaacata
     5701 tcaagcacaa cgtgtgttaa cagatatttt accagcaata gacaatatag aacgtgcact
     5761 tcaaattgaa ggtgatgatg agacttttaa atctcttcaa aaaggtgtac aaatggtgca
     5821 tgaaagtttg attaacgcac taaaagataa tggtcttgaa gttattaaaa ctgaaggtga
     5881 agcatttgat ccaaatattc accaagctgt agttcaagat gataaccctg attttgaatc
     5941 tggcgaaatc actcaagaac tacaaaaagg atacaagctt aaagatagag tattaagacc
     6001 atcaatggtc aaagtaaacc aataacttaa atttggcgaa aagacattgt ttaaaattaa
     6061 attaatttaa tgattaaatg gaggaatttt attatgagta aaattattgg tatagactta
     6121 ggtacaacaa attcatgtgt aacagtatta gaaggcgatg agccaaaagt aattcaaaac
     6181 cctgaaggtt cacgtacaac accatctgtt gtagctttca aaaatggaga aactcaagtt
     6241 ggtgaagtag caaaacgtca agctattaca aacccaaaca ctgttcaatc tattaaacgt
     6301 catatgggta ctgattataa agtagatatt gaaggtaaat catacacacc acaagaaatc
     6361 tcagctatga ttttacaaaa cttaaaaaat acagctgaaa gctatttagg tgagaaagtt
     6421 gacaaagctg taattacagt acctgcatac tttaacgatg ctgaacgtca agcaactaaa
     6481 gatgctggta aaattgctgg tttagaagtt gagcgtatca ttaatgaacc aacagctgca
     6541 gcattagcat atggtttaga caaaactgat aaagatgaaa aagttcttgt ttttgactta
     6601 ggtggcggta catttgacgt atctatccta gaattaggtg acggtgtatt cgaagtacta
     6661 tcaacagccg gtgacaacaa acttggcggt gatgattttg accaagtaat tattgactac
     6721 ctagttgcag aattcaaaaa agaaaatggc gtagacttat ctcaagataa aatggcatta
     6781 caacgtttga aagatgctgc tgaaaaagct aaaaaagact tatcaggtgt atcacaaact
     6841 caaatctcat taccatttat ctcagctggt gaaaacggtc cattacactt agaagtaaac
     6901 ttaactcgtt ctaaatttga agaattatca gattcattaa ttagaagaac aatggaacct
     6961 acacgccaag caatgaaaga cgctggctta acaaactcag atatcgatga agttatctta
     7021 gttggtggat caactcgtat tccagcagta caagaagctg tcaaaaaaga aatcggtaaa
     7081 gagcctaaca aaggagtaaa cccggacgaa gtagtggcaa tgggagctgc aatccaaggt
     7141 ggcgttatca caggtgacgt taaagacgta gtattattag acgtaacacc actatcttta
     7201 ggtattgaaa ttttaggtgg acgtatgaat acgttaattg aacgtaacac tacgattcct
     7261 acatctaaat ctcaaatcta ttcaacagca gtagataatc aaccatcagt agatgtacac
     7321 gtattacaag gtgaacgtcc aatggctgcg gataataaaa cacttggtag attccaattg
     7381 actgatattc caccagctga acgtggtaaa cctcaaattg aagtaacgtt tgatatcgat
     7441 aaaaacggta ttgtaaatgt aactgcaaaa gacttaggta caaataaaga acaaagaatt
     7501 acaattcaat caagttcttc attatcagac gaagaaatcg accgtatggt aaaagatgct
     7561 gaagttaacg ctgaagcaga taaaaaacgt cgtgaagaag tagacttaag aaacgaagct
     7621 gacagtctag tattccaagt tgaaaaaact ttaactgatt taggcgaaaa tatcggtgaa
     7681 gaagataaaa aatctgctga agagaaaaaa gacgctctta aaactgcttt agaaggtcaa
     7741 gatatagaag atattaaatc taaaaaagaa gaacttgaaa aagtgattca agaattatca
     7801 gcaaaagtat atgagcaagc ggctcaacag caacaacaag cacaaggtgc taatgctggt
     7861 caaaacaatg atagtactgt agaagatgct gaatttaaag aagtaaaaga cgacgacaaa
     7921 aaataattaa acaatacaat tagaatgttg tcatgatctg aaaagtcaaa gtcaatagaa
     7981 cattggcttt ggctttttat ttggatagta tgacatatta cgttaaacta tatgagttaa
     8041 ttaagaaaag gagagatagc tgtggccaaa agagattatt atgaagtttt aggcattagt
     8101 aaggatgctt caaaagatga aatcaaaaaa gcgtatcgaa agctttcgaa aaaatatcat
     8161 ccagatatta acaaagaaga aggtgcagat gaaaagttta aagaaatttc tgaagcctat
     8221 gaagttttaa gtgatgataa taaacgcgca agctatgatc aatttggaca tgatggtcca
     8281 caaggttttg gtggacaagg attcaatggc tctgactttg gcggttttag tggcttcggt
     8341 ggcggcggtt ttgaagatat ctttagctct ttcttcggtg gcggcagaca aagagatcca
     8401 aatgcaccgc aaaaaggtga tgatcttcag tatacaatga cattaacatt tgaagaagcg
     8461 gtatttggta caacaaaaga aatttcaatc cgtaaagatg taacatgcga aacatgtcat
     8521 ggtgatggtg caaagcctgg aacaagtaaa aagacttgta gttactgtaa tggcgctggt
     8581 catgtagctg tagaacaaaa cacaatttta ggtagagttc gtactgaaca agtttgtcct
     8641 aaatgtaatg gaagtggtca agaatttgaa gaagcttgtc caacatgtca cggaaaaggt
     8701 actgaaaata aaacagttaa attagaagtg aaagtacctg aaggtgtcga caatgaacaa
     8761 caaattagat tagctggtga aggctctcca ggcgtgaatg gaggacctgc tggtgattta
     8821 tacgtagtat ttagagttaa accatctgaa acatttaaac gtgatggtga tgatatttat
     8881 tacaaattaa atgttagctt cccacaagca gctttaggcg atgaaataaa aataccaaca
     8941 ttaaataatg aagttatgtt aacaatccct gctggtacac aaacaggtaa gcaattccgc
     9001 ttaaaagaaa agggtataaa aaatgttcat ggctatggat acggagattt atatgttgat
     9061 attaaagtag taacgcctac taaattaaca gatagacaaa aagaactaat gaaagaattt
     9121 gcccaattaa atggtgaaga aattaatgaa caaccttcga attttaaaga tagagcaaaa
     9181 agattcttta agggagaata aagtatgaac tggacagagc tttcaattat tattaatcat
     9241 gaagcagtag aattggctac caatatactt gaaaatcatg gatcaaatgg tgtcgtgata
     9301 gaagattcag atgatttaat taaccaacca gaagataaat acggtgaaat ttacgctttg
     9361 aaaaaagagg attatccaga taagggagta agattgaaag cctattttaa tgaaatgact
     9421 tatgatgata agttgcgaca gcaaattaaa gatgagttat taaatttaga tgaacttgat
     9481 caacataacg ttcaattcag tgagcaaatt attgcagaga cggattggga aaatgaatgg
     9541 aaaaactatt tccatccatt ccgagcgtcg aagaagttca caatagttcc tagttgggaa
     9601 acatatgcta aagaagcgga tgaagagctt tgcattgagc tcgacccagg tatggctttt
     9661 ggaacaggtg atcatccgac tacaagtatg tgtttgaagg caatagaaac atatgtattg
     9721 ccacagcatt cagtaattga tgttggtact ggctcaggta tattaagtat tgcaagtcat
     9781 ctaatcggtg taaaacgtat taaagcgttg gatattgatg aaatggcagt gagtgtagct
     9841 aaagaaaact tcagaagaaa tcattgtgaa acgttaattg aagctgttcc aggtaactta
     9901 ttgaaagacg aaacagaaaa atttgatatt gtaatagcaa atattttagc gcatattatt
     9961 gatgaaatga ttgaagatgc ttataatact ctaaatgaag gcggttattt tattacttct
    10021 ggtattataa aagagaagta tgaaggtata cagtcacata tggagcgtgt aggttttaaa
    10081 attatttcag aacaacatga caacggttgg gtttgtcttg ttggccagaa agtgagtgaa
    10141 taatgtgcaa cgttatttca tagaccaaaa cgctgatgta agtcagcgtt tttttattac
    10201 aaaaaaagaa gatattcatc atataacaaa tgtaatgaga tatgatgttg gtagtaaaat
    10261 tattttaact tttaatgatc aaacagttta tttatgtgaa attgctgaca taacaagtga
    10321 taaaattgaa gttgatttaa aagaaaaaca aaatattaat acagaattgc cagttgatgt
    10381 tacgatttgc agtggactaa tcaaagctga caaatatgag tggttgctac aaaaagctac
    10441 tgaattgggt gcttcatcat ttatagctgt gagcatggaa cgttcaattg ttaaattaaa
    10501 cgaggcaaaa gttgccaaaa agatagagcg ttggcaaaaa ataattaaag aagctgctga
    10561 acaaagctat cgtttagtga ttccatctat tcaattcgag tcgaatttaa aattaatttg
    10621 tgatactatt gataattatg actatattct tattgcatat gaagaggaag caaaagatgg
    10681 tgagttaagc aatttcaagc aaactttaca acaattcaat gctcaggata aagtgttgat
    10741 gatttttggt cctgagggtg gtttgtcaga aaatgaaatt tcattattta gtaatactag
    10801 tacagttgtt ggtcttgggc cgagaatttt acgggcagaa actgcgccat tgtatgcgtt
    10861 aagtgcaata agttatgaaa aagaattaat ggggtgaata tttatgtcaa cagttgcgtt
    10921 tcacacatta ggttgtaaag taaaccatta tgaaactgaa gcaatctggc aattatttaa
    10981 agaagcaaac tatgagcgcg ttgactttga agcgaatgct gatgtatttg ttattaatac
    11041 ttgtacagta acgaatacgg gtgataaaaa aagtcgtcaa ataattagac gtgcaataag
    11101 acaaaatcct gatgctgtaa tctgtgtaac aggttgttat gcgcaaactt catcagctga
    11161 aattatggaa attcctggtg tcgatgtagt agttggtaca caagatagac ataaactatt
    11221 aggttacatt gacgaattcc gtaaagaacg ccaaccaatt aatggtgttg gaaatatcat
    11281 gaaaaatcgt aaatatgaag aattagatgt cccttatttt acagatagaa cacgtgcgtc
    11341 attaaaaatt caagaaggtt gtaacaactt ctgcacattc tgtattattc catgggctcg
    11401 tggcttaatg cgttcaagag atccggaaaa agtagttgaa caagcgacgc aactagtgaa
    11461 ttcaggatat aaggaaattg tattgacggg aattcataca ggtggatatg gtcaagattt
    11521 aaaagattat aacttggccc aattattacg tgatcttgaa acgattaatg gattagaacg
    11581 aattcgaatt tcttcaattg aagcaagtca acttacagat gaagtaattg acgttttaga
    11641 acgttcaaca aaagttgtgc gtcatttgca tattccatta caatctggtt cagatacagt
    11701 attaaaacgt atgagacgta agtatacaat ggatagattt tcagaacgat taacaaaatt
    11761 gcataaagct ttaccagact tggcagttac gagtgatgta attgttggtt tcccaggtga
    11821 aactgaagct gagttccaag aaacatatga ttttatcgta aagcataagt tctctgaact
    11881 gcatgttttc ccttattctc ctagaattgg cacgccagct gcaagaatgg atgaccaaat
    11941 tgatgaagaa attaaaaatg aacgggtgca taagttaatt acgctaagca atcaactcgg
    12001 aaagttatat gcgtctaaat ttgatcaaga tgtgcttgaa gtaattcctg aggaacaggg
    12061 tgacacagaa ggtacattag ttggatatgc agataattat atgaaagtac aatttgaagg
    12121 tgacgaatca ctcataggtc aaattgtaaa agttaaaatt acgcaagcaa attatccatt
    12181 aaatgaaggg caagcaatta aagttgttga tttcgcaaca aataaatctg atagagaagt
    12241 tttagtttaa gtattccttg attcatacca tatggttccg tattaaagat ataggttttg
    12301 taacgttgtt ttaacggctt cacaatttac tatataagca actcttctca ttaaataaat
    12361 tattaagaga tttttagaac aaaatctaaa agtctttgtg attttgatgt gaaatatgtt
    12421 tcaaataaaa aacaatttac taattgacca taaattacag atatattata cttataaatg
    12481 catagtttta ctgtgcaatt gactataaag ttccgttgat atttggaggg agggaaatac
    12541 agatgtctaa aacagtagta cgtaaaaatg aatcacttga agatgcgtta cgtagattta
    12601 aacgttcagt ttctaaaagt ggaacaatcc aagaagtacg taaacgtgaa ttttacgaaa
    12661 aaccaagcgt aaaacgtaaa aagaaatcag aagctgcacg taaacgtaaa ttcaaataat
    12721 taatacctct gttgactccc tcaacacgaa tattaattat ataaaacaaa catcacaagt
    12781 tagt
//
LOCUS       DAKTIO010000072        12723 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.072, whole genome shotgun sequence.
ACCESSION   DAKTIO010000072 DAKTIO010000000
VERSION     DAKTIO010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12723
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.072"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(189..1460)
                     /locus_tag="O0293_001486"
     CDS             complement(189..1460)
                     /locus_tag="O0293_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCY0280374.1"
                     /translation="MNYLESLYWIHERTKFGIKPGVKRMEWMLAQFNNPQNNIKGIHV
                     GGTNGKGSTVAYLRTALVENGYEVGTFTSPFIETFNERISLNGVPISNDAIVELVSRI
                     KPVSEMMERETDLGVATEFEIITAMMFLYFGEIHPVDFVIVEAGLGIKNDSTNVFTPV
                     LSILTSIGLDHTDILGGTYLDIARDKGAIIKPNVPVIYAVKNEDALKYVRERAIEQHA
                     KPIELDREIVVVSQNDEFTYRYKDYELETIILSMLGEHQKQNAALAITALIELNEQGL
                     IELDFNKMIDGIESVRWTGRIEQVHDKPLIILDGAHNSESIDALIDTIKQYHDKEKVD
                     ILFSAINGKPINEMVKHLSLIAHTFYATEFDFPKALRKEEIVGSIENDEIQLVDDYVE
                     FIKNYQGDTLVITGSLYFISEVKSKLDFEAL"
     gene            complement(1473..4103)
                     /locus_tag="O0293_001487"
     CDS             complement(1473..4103)
                     /locus_tag="O0293_001487"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCY0280375.1"
                     /translation="MEMKPKYDPREVEAGRYEEWVKNGYFKPSEDKSKETYTIVIPPP
                     NVTGKLHLGHAWDTTLQDIITRMKRMQGYDTLYLPGMDHAGIATQAKVEAKLNEQGIT
                     RYDLGREKFLEQAWDWKEEYASFIRAQWAKLGLGLDYSRERFTLDEGLSKAVKKVFVD
                     LYNKGIIYRGERIINWDPKARTALSDIEVIHEDVQGAFYHFKYPYADGEGFIEIATTR
                     PETMLGDTAIVVNPNDERYKDVIGKTVILPIVGRELPILADEYVDIDFGSGAMKVTPA
                     HDPNDFEIGQRHQLENIIVMDENGKMNDKAGKYEGMDRFDCRKQLVKDLKEQDLVIKI
                     EDHVHSVGHSERSGAVVEPYLSTQWFVRMEDLAKRSLDNQKTDDRIDFYPQRFEHTFN
                     QWMENIRDWTISRQLWWGHQIPAWYHKETGEIYVGEEAPTDIENWQQDEDVLDTWFSS
                     ALWPFSTLGWPDLESEDFKRYYPTNALVTGYDIIFFWVARMIFQGLEFTDRRPFNDVL
                     LHGLVRAEDGRKMSKSLGNGVDPMDVIDEYGADSLRYFLATGSSPGHDLRYSTEKVES
                     VWNFINKIWNGARFSLMNIGEDFKVEDIDLSGNLSLADKWILTRLNETIATVTDLSDK
                     YEFGEVGRALYNFIWDDFCDWYIEMSKIPMNSNDEEQKQVTRSVLSYTLDNIMRMLHP
                     FMPFVTEKIWQSLPHEGDTIVKASWPEVRESLIFEESKQTMQQLVEIIKSVRQSRVEV
                     NTPLSKEIPILIQAKDKEIETTLSQNKDYLIKFCNPSTLNISTDVEIPEKAMTSVVIA
                     GKVVLPLEGLIDMDKEISRLEKELAKLQSELDRVDKKLSNENFVSKAPEKVINEEKRK
                     KQDYQEKYDGVKARIEQLKA"
     misc_binding    complement(4147..4448)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4506..5066
                     /locus_tag="O0293_001488"
     CDS             4506..5066
                     /locus_tag="O0293_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCY0280376.1"
                     /translation="MNECAFGTKDPVYLDYHDHVWGQPLYDSKALFKLLALESQHAGL
                     SWLTILKKKEAYEEAFYDFEPEKVAQMTAQDIDRLMTFPNIVHHRKKLEAIVNQAQGY
                     LKIEQAYGSFSKFLWSYVNGKPKDLQYEHASDRITVDDTATQLSKDLKQYGFKFLGPV
                     TVFSFLEAAGLYDAHLQDCPSKPKHN"
     gene            complement(5289..6362)
                     /locus_tag="O0293_001489"
     CDS             complement(5289..6362)
                     /locus_tag="O0293_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCY0280377.1"
                     /translation="MAMIYRNNFIVFVLSFFISILLYSSHVLLPFMFGPIIASIICVK
                     VFKLDIKWPFLLSELGIVLLGVQIGSTFTKNVVMDIKNNWLSIIVVSISILLIAIVMA
                     FVFKKIARINTETAILSVIPGALTQMLVMAEQDKRANLLVVSLTQTSRIIFVVVLVPF
                     ISYFFHDGNMHANGKLTKVLPLSQVLNIGQIVILAIAIFIVYLIMSKIKFPTFQLLAP
                     LIVLIVWNFSTGLTFTLDHWLLNMAQLIYMIRIGVQIAHLLSDLKGRLAIAITIQNIM
                     LIIGALIMVYIIHFFDNNPINELFLGAAPGGVNQIVLVALATGADVAMITSYHIFRIF
                     FILFIIAPGINYFLKYRSNKRED"
     gene            complement(6396..6497)
                     /locus_tag="O0293_001490"
     CDS             complement(6396..6497)
                     /locus_tag="O0293_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280378.1"
                     /translation="MGGDLIVKVHICFVVKTASGYCYLNKREAQAAI"
     gene            complement(6558..7844)
                     /gene="hemL"
                     /locus_tag="O0293_001491"
     CDS             complement(6558..7844)
                     /gene="hemL"
                     /locus_tag="O0293_001491"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCY0280379.1"
                     /translation="MRYTKSEEAMKVAETLMPGGVNSPVRAFKSVDTPAIFMDHGKGS
                     KIYDIDGNEYIDYVLSWGPLILGHRDPQVISHLHEAIDKGTSFGASTLLENKLAQLVI
                     DRVPSIEKVRMVSSGTEATLDTLRLARGYTGRNKIVKFEGCYHGHSDSLLIKAGSGVA
                     TLGLPDSPGVPEGIAKNTITVPYNDLDALKIAFEKFGNDIAGVIVEPVAGNMGVVPPI
                     EGFLQGLRDITTEYGALLIFDEVMTGFRVGYHCAQGYFGVTPDLTCLGKVIGGGLPVG
                     AFGGKKEIMDHIAPLGNIYQAGTLSGNPLAMTSGYETLSQLTPETYEYFNMLGDILED
                     GLKRVFAKHNVPITVNRAGSMIGYFLNEGPVTNFEQANKSDLKLFAEMYREMAKEGVF
                     LPPSQFEGTFLSTAHTKEDIEKTIQAFDTALSRIVK"
     gene            complement(7892..8866)
                     /gene="hemB"
                     /locus_tag="O0293_001492"
     CDS             complement(7892..8866)
                     /gene="hemB"
                     /locus_tag="O0293_001492"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001633925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCY0280380.1"
                     /translation="MKFDRHRRLRSSATMRDMVRENHVRKEDLIYPIFVVEKDDVKKE
                     IKSLPGVYQISLNLLESELKEAYDLGIRAIMFFGVPNSKDDIGTGAYIHDGVIQQATR
                     IAKKMYDDLLIVADTCLCEYTDHGHCGVIDDHTHDVDNDKSLPLLVKTAISQVEAGAD
                     IIAPSNMMDGFVAEIRRGLDEAGYYNIPIMSYGVKYASSFFGPFRDAADSAPSFGDRK
                     TYQMDPANRLEALRELESDLKEGCDMMIVKPALSYLDIVRDVKNHTNVPVVAYNVSGE
                     YSMTKAAAQNGWIDEERVVMEQMVSMKRAGADMIITYFAKDICRYLDK"
     gene            complement(8869..9537)
                     /locus_tag="O0293_001493"
     CDS             complement(8869..9537)
                     /locus_tag="O0293_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCY0280381.1"
                     /translation="MKPVVVMTQTNDMQSDLVSIIHKPFIDIKPLNFDIHLLNQRYDW
                     LIFSSKNAVKFFYKYLKGINVDNIAVIGSKTAQYCESLGIRVDFMPNDFSQEGFLKSF
                     NQTNQKILLPSSELARPLLLAALSKDNEVVKIDLYTSVPNKQNIQDVKEMIEHQQIDA
                     LTFSSSSAVRYYFNEGFVPKFKSYFAIGEQTARTIKSYQQPVTIAEIQTLESLIEKIL
                     ESRG"
     gene            complement(9559..10485)
                     /gene="hemC"
                     /locus_tag="O0293_001494"
     CDS             complement(9559..10485)
                     /gene="hemC"
                     /locus_tag="O0293_001494"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCY0280382.1"
                     /translation="MRKLVVGSRRSKLALTQSQQFIDKLKAVEPNLEIEIKEIVTKGD
                     RIVDKQLSKVGGKGLFVKEIQHELFEKNIDMAIHSLKDVPSVIPEGLTLGCIPDRELP
                     FDAYISKTHTPLSQLPEGSIIGTSSLRRGAQILSKYPNLEIKWIRGNIDTRLEKLQTE
                     DYDAIILAAAGLRRMGWSDDIVTSYLDRDTLLPAIGQGALGIECRSDDEELLTLLSKV
                     HNDEVAKCVTAERTFLAEMDGSCQVPIAGYATISDQNEIEFTGLIMTPDGKERFEYTM
                     NGTDPVELGKTVSNKLKEQGAYEIIKRLNEQH"
     gene            complement(10527..11342)
                     /gene="ccsA"
                     /locus_tag="O0293_001495"
     CDS             complement(10527..11342)
                     /gene="ccsA"
                     /locus_tag="O0293_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HCY0280383.1"
                     /translation="MQENLFIRFNEIILLIYLISIICYFYDFVQKSHKIRSLGIYLLG
                     IVWVLQTISLSIFIIQTRHIPLGSISDVFYTLSWLIISISLILNLIKVLNFSVFFLNL
                     IGLTLLGMNTFQPTHYTNKVQKIAVVDELLLVHIGLAVLSYVFFALAFVNALLYIMQY
                     RNLKEKRFDQKYFRIGSVATLESIVFYSTLSGWIILIFSIILGTQWGVISVGERIFID
                     PKVILSSIITVLYGSYILLRINKWLNSRYLIYYNIILFCLNMINLFFATHFVN"
     gene            complement(11364..12710)
                     /locus_tag="O0293_001496"
     CDS             complement(11364..12710)
                     /locus_tag="O0293_001496"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCY0280384.1"
                     /translation="MHFIAISINHRTADVALREQVAFRDDALRIAHEDLYETKSILEN
                     VILSTCNRTEVYAVVDQIHTGRYYIQRFLARAFGFEVDDIKAMSEVKVGDEAVEHLLR
                     VTSGLDSIVLGETQILGQIRDAFFLAQSTGTTGTIFNHLFKQAITFAKRAHNETDIAD
                     NAVSVSYAAVELAKKVFGKLKSKQAIIIGAGEMSELSLLNLLGSGITDITVVNRTIEN
                     AMKLAAKHQVKYDELSSLPNLLESADIVISSTSAQSYIITNEMIERIAENRKQDSLVL
                     IDIAVPRDIEPGISAITNIFNYDVDDLKGLVDANLRERQLAAATISEQIPAEIHAHNE
                     WISMLGVVPVIRALREKAMAIQAETMDSIDRKLPGLSERERKIISKHTKSIINQMLKD
                     PIKQAKELSSDKKSNEKLELFQNIFDIEAECPHEQAKQQKESKVKEISARRIFSFE"
BASE COUNT         4091 a         2461 c         1746 g         4425 t
ORIGIN      
        1 ttcatttaaa tttttcaata ttgctaagca taattgatgg atactttaac aacccattac
       61 tgctcggcaa aattaataat ggcaagaaat tgaaccttat aaacacatac gatttagagc
      121 ataaaaaata accatgaagc tctacctatt gattaaatag attcttcatg gctattttag
      181 ttttagtttt ataatgcttc aaagtctaat tttgatttaa cttcacttat gaaatacaga
      241 ctaccggtaa ttactaatgt atcaccttga taatttttta taaattcaac gtagtcatct
      301 actaattgta tttcatcatt ttcaatacta cctacaattt cttctttgcg taacgctttc
      361 ggaaaatcaa attcagttgc ataaaacgta tgcgcaatta aacttaaatg tttgaccatc
      421 tcgttaatcg gttttccgtt tattgctgag aacaaaatat ctactttttc tttatcatgg
      481 tactgtttaa ttgtatcaat tagagcatct atactctctg aattatgtgc gccatccaaa
      541 atgattaaag gtttgtcatg cacctgctca atacgtccag tccaacgaac tgattcaata
      601 ccgtctatca tcttattgaa atctaattca attaatcctt gttcatttaa ttcaataaga
      661 gctgttatgg ctaatgcagc attttgtttc tgatgttcac ctaacatgct taaaatgatt
      721 gtttctaatt cataatcttt ataacggtaa gtaaattcat cattttgcga tacaacaaca
      781 atttctctat ctaattcaat tggctttgca tgttgttcaa ttgcgcgttc acgaacatat
      841 tttaatgcat cttcattttt aacagcatat atcactggaa cgttaggctt tataatcgcg
      901 cctttatccc tagcaatatc tagataagta ccacctaaaa tatctgtatg gtctagaccg
      961 atactagtta agattgataa aaccggtgta aagacatttg tcgaatcgtt ctttataccc
     1021 aatccagcct caacaatgac aaaatcaaca ggatgtattt caccaaaata taaaaacatc
     1081 atcgctgtga ttatttcgaa ttcagttgca acacctaaat ctgtttcacg ttccatcatt
     1141 tcacttactg gtttaatacg tgatactaat tctacaatag cgtcatttga tattggcaca
     1201 ccatttagac taattcgttc attaaatgtt tcaataaacg gcgacgtaaa tgtacctact
     1261 tcataaccat tttcaactaa agctgttcta aggtaagcaa ctgtagagcc tttaccattt
     1321 gtgccaccta catgaatacc cttaatgtta ttttgaggat tattaaattg tgctagcatc
     1381 cattccatac gtttaacacc tggtttgatg ccaaatttag ttctttcgtg tatccaatac
     1441 aagctctcta ggtaattcat tgttactaac tcctatgctt ttaattgttc aattcttgcc
     1501 ttcacaccat catatttttc ttgataatct tgttttttac gtttttcttc atttataacc
     1561 ttttcaggtg ctttacttac aaagttttca ttagagagct ttttatctac tctatctaat
     1621 tcgctttgaa gtttagctaa ttctttttcc aaacggctga tttccttatc catatcaatt
     1681 agcccttcta atggtaatac cactttacct gcaattacaa ctgatgtcat tgctttctca
     1741 ggaatttcca cgtcagtgct aatatttaag gtactaggat tacagaattt gattaaataa
     1801 tctttgtttt gtgataaagt tgtttcaatt tctttatctt tagcttgaat taaaataggt
     1861 atttctttag acaatggcgt atttacttct acacgtgatt gtcttacaga tttaatgatt
     1921 tcaacaagtt gttgcattgt ttgtttactt tcttcaaaaa tcaatgattc acgcacttct
     1981 ggccatgaag ctttaacaat tgtgtcacct tcatgtggta aactttgcca tattttctct
     2041 gttacaaatg gcatgaatgg atgtagcatt ctcataatat tgtctaaagt ataactcaat
     2101 actgaacgtg taacttgttt ttgttcttca tcattactat tcattggaat tttactcatt
     2161 tcaatgtacc aatcacagaa atcatcccaa atgaaattat ataatgcacg tccaacttcg
     2221 ccgaattcat atttgtcact taaatcagta actgttgcaa tcgtttcatt taaacgtgtt
     2281 agaatccatt tatctgctaa tgataagtta ccacttaaat cgatatcttc aactttaaag
     2341 tcttcaccga tattcattaa actgaaacgt gccccattcc agattttatt gataaagttc
     2401 cacactgact caactttttc agttgagtat cttaaatcat gtcctggaga tgaacctgtt
     2461 gctaagaagt aacgcaagct atcagcaccg tattcgtcaa taacatccat tggatccaca
     2521 ccattaccta atgatttact catcttacgc ccgtcttcag cacgaactaa accgtgtaat
     2581 aatacatcat taaatggacg acgatctgta aattctaagc cttggaatat catgcgtgct
     2641 acccagaaaa agataatatc gtaacctgta actaaggcat ttgttgggta gtatcgttta
     2701 aagtcttcac tttctaaatc aggccaacct aacgtagaga aaggccataa agcacttgag
     2761 aaccacgtat ctaatacatc ttcatcttgt tgccaatttt caatatcagt tggcgcttct
     2821 tctccaacat atatttcgcc tgtttcttta tgataccaag ccggaatttg atgaccccac
     2881 cataattgtc ttgaaatcgt ccaatctcta atattttcca tccattggtt aaatgtatgt
     2941 tcgaaacgtt gcggataaaa atcaatacga tcatctgttt tttggttatc taatgaacgt
     3001 ttcgctaagt cttccatgcg cacaaaccat tgtgttgata aatatggttc aacaacagcg
     3061 ccagatcgtt ctgaatgacc tacagaatga acatgatctt caatcttgat aactaaatct
     3121 tgttctttta aatctttaac tagctgttta cgacaatcaa aacggtccat accttcatat
     3181 ttacccgctt tgtcgttcat tttaccattt tcatccataa cgataatatt ttctaattga
     3241 tgtctttgac caatttcaaa atcattaggg tcatgtgctg gtgtcacttt catagcacca
     3301 gaaccgaagt ctatatcaac atactcatct gctaaaatag gcagttcgcg tcctacgatt
     3361 ggtaatataa cagttttacc gattacatct ttgtatcgtt cgtcattagg gttaacaaca
     3421 atcgctgtat cacctaacat cgtttctggt cttgttgttg caatttcaat aaaaccttca
     3481 ccatcagcgt aaggatattt aaaatgataa aacgcacctt gaacatcttc atgtattact
     3541 tcaatatcag ataaagctgt acgtgcttta ggatcccaat ttataatacg ttcgccacga
     3601 taaataattc ctttattgta taaatcaaca aaaacttttt taactgcttt acttaaacct
     3661 tcatctaaag taaaacgttc tctactataa tctaaaccta gacctaattt agcccattgc
     3721 gcacgaataa atgacgcata ctcttctttc caatcccatg cctgttctaa aaacttttca
     3781 cgaccaagat catatctagt tattccttgt tcatttaatt tagcttctac ctttgcctgt
     3841 gtcgcaatac cagcatgatc catacctggt aagtataacg tatcgtatcc ttgcatacgt
     3901 ttcatacgtg taatgatatc ttgtaaagtc gtatcccatg catgtcctaa atgtaattta
     3961 ccagttacat ttggtggcgg gataacaatt gtatatgttt cttttgattt atcttctgac
     4021 ggtttaaaat aaccattctt tacccattct tcataacgtc ccgcttcaac ttcacgagga
     4081 tcatattttg gtttcatttc catttgctat acctcctaaa aaaataaaaa tatccatcct
     4141 atatacaaat aggacggata ttccgtggta ccacctatat tcaagaagga tgattaatat
     4201 caaattcact cttttaacat aattggaata atcataccaa tactatcatc gtgaaatttg
     4261 aaatgcttca tctcttcaag cactctagat tatgattaac gctcaaacac gtcttagcct
     4321 actattaatc acgttcagct aagatactct gtgggctacc ttcagtaaaa atcatttaca
     4381 tactcacacc aaatcatatg ctctctttaa aataatttga acttactctt cccaaatcct
     4441 atattaaact cttaacttat agtataatga ttgacaaaat aagtcaatgt ataggtggga
     4501 ataaaatgaa tgaatgcgca tttggtacta aagatccagt ctacttagac tatcatgatc
     4561 atgtatgggg acaaccgctc tatgatagca aggcattgtt taaactttta gcattagaat
     4621 cacaacatgc tgggctatct tggttaacta ttttaaaaaa gaaagaagcc tatgaagaag
     4681 cattttatga tttcgaacca gaaaaggtag cacaaatgac cgctcaagat atcgaccgct
     4741 taatgacttt tccaaatatc gttcatcatc gtaaaaaatt agaagcaatt gttaatcaag
     4801 ctcaagggta tttaaaaatt gaacaagcat atggtagttt tagtaaattt ttatggtcat
     4861 atgtaaatgg taagcctaaa gatttgcagt atgaacatgc ttctgatcgt atcacagttg
     4921 atgatactgc aacacaacta tctaaagatt taaaacaata cgggtttaaa tttttaggtc
     4981 cagtaacagt attttcgttt ttagaagcag ccggtttata tgatgcacat ttacaagatt
     5041 gtccatcaaa gcctaaacat aattaatttg ctatagcatc ataaagtaaa cgagacatca
     5101 tctattttta actaatagac tgtctcgttt tttaatgtga aaatttattc aaatcataag
     5161 ttaaagttcc aagttaatgc tctgctaata ttagatatct taatctaaaa atagatgtac
     5221 aacctttgct taaaccttaa taatgaattc tatcctaagc ataattcaac ttagatggat
     5281 gatagtttct aatcttcacg cttattcgaa cgatatttta aaaagtaatt aattcctggt
     5341 gcaataataa ataggatgaa aaagattcgg aaaatatgat aactcgtaat catagcaaca
     5401 tcggcaccag tagctaatgc aactaaaact atctgattaa cccctcctgg tgctgcacca
     5461 agaaacaatt cattaatagg attattatca aagaaatgta tgatataaac catgattagc
     5521 gcaccaatta tcaacataat attttgaatt gtaattgcga ttgctagtct accttttaaa
     5581 tctgacaata aatgcgcaat ttgaactcca attctaatca tatatattag ttgtgccatg
     5641 ttcaacaacc aatgatctag tgtaaatgtt aaacctgtag aaaaattcca aacaattaat
     5701 acaatgagtg gtgctaataa ttgaaatgtt ggaaacttta ttttagacat aattagataa
     5761 actataaaga tagctatcgc taaaataact atttgcccta tgtttaatac ttgtgataaa
     5821 ggcaagactt ttgttaactt tccattcgca tgcatgttac catcatgaaa aaaatatgaa
     5881 atgaacggta ctaaaacaac aacaaatata attcgtgatg tttgcgttaa gctaacaact
     5941 aacaaattag cacgtttgtc ttgttcagcc atgaccagca tttgtgttag tgctcctggt
     6001 ataacactta aaatagctgt ttctgtatta atacgtgcaa tttttttaaa aacaaatgcc
     6061 attactattg caattaataa tatcgaaata gatacaacaa taatcgaaag ccaattgttt
     6121 ttaatatcca taacgacatt tttcgtaaac gttgatccga tttgcacacc taatagtaca
     6181 atacctaatt cactaagtaa gaatggccat ttaatatcaa gtttgaaaac ttttacacaa
     6241 atgattgatg cgataatagg accaaacata aatggaagta atacgtgcga tgaatacagt
     6301 agaatactaa taaaaaatga taaaacgaac acaatgaaat tatttctata tatcattgcc
     6361 atgttttcca cttctttcaa taaaaaataa aatgactaaa ttgctgcttg agcttcacgt
     6421 ttgttaagat aacaatatcc gctagcagtt ttgactacaa agcatatatg gactttcact
     6481 atcaagtcgc cgcccatgcc ttatatacat ttaaaaagag cctgaacaaa gttcaggctc
     6541 tcaatttgtc cgtatattta ttttacaata cgacttaaag ccgtatcaaa tgcttgaatc
     6601 gttttttcaa tatcttcttt cgtgtgtgcc gtagataaga atgtaccttc aaattgagat
     6661 ggtggtaaaa acacaccttc ttttgccatt tctcgataca tttctgcaaa taatttcaaa
     6721 tcacttttat tcgcttgttc aaaattagtt acaggtcctt catttaagaa ataaccaatc
     6781 attgaacctg ctctatttac agttattggt acattgtgtt tagcaaatac acgttttaaa
     6841 ccgtcttcaa gtatatcgcc taacatatta aaatactcat atgtctctgg cgttaattgg
     6901 cttaacgttt cataaccact tgtcattgca agaggatttc ctgataacgt acccgcttga
     6961 taaatatttc ctaatggtgc tatatgatcc atgatttctt ttttaccacc aaatgcacct
     7021 acaggtagtc ctccaccgat aacttttcct aagcaagtta aatctggtgt cacaccaaag
     7081 taaccttgtg cacaatgata accgactctg aaaccagtca ttacttcatc gaaaattagc
     7141 aatgcgccgt attcagtcgt aatatctctt aatccctgta aaaaaccttc aatcggcggt
     7201 acgacaccca tattaccagc aacaggttct acgattacac cagcaatatc gtttccaaat
     7261 ttttcgaaag cgattttaag tgcatctaaa tcattgtatg gaactgtaat tgtattttta
     7321 gcaatacctt caggcacacc aggagaatcc ggcaatccta atgttgccac cccagaacca
     7381 gctttgatta ataacgaatc actatgacca tgatagcaac cttcaaattt cacaatttta
     7441 tttctgccag tataaccacg tgctaatctt aaagtatcca atgtagcttc tgtaccagat
     7501 gacaccatac gcactttttc tattgaaggt actcggtcaa taacgagctg cgccaattta
     7561 ttttcaagta atgttgatgc accaaaactt gtacctttat caattgcttc atgtaaatga
     7621 ctaataactt gagggtctct atgtcctaaa ataagtggtc cccaacttag tacatagtcg
     7681 atatactcgt taccatcgat atcataaatt tttgaacctt taccgtgatc cataaaaatt
     7741 gctggtgtat ctactgattt aaatgcgcgt actggactat ttacaccacc aggcattaaa
     7801 gtttcagcaa ccttcattgc ttcttctgat ttcgtatatc tcataaagta aattcctcct
     7861 acagtttatg gaaaatcata aatataaaac cttatttatc taaatagcga caaatgtcct
     7921 ttgcaaaata cgtaataatc atatcagcac ctgcacgttt cattgaaacc atttgttcca
     7981 taacgacacg ttcttcatct atccaaccat tttgtgccgc tgctttagtc atactatatt
     8041 ctccactcac attatatgca acaactggaa cattcgtatg atttttaaca tctcgaacta
     8101 tatctaaata acttagagca ggtttaacaa tcatcatgtc gcacccttct ttaagatcac
     8161 tttctaattc acgaagtgct tccaaacggt tagcagggtc catctgatac gtttttctat
     8221 ccccaaatga tggcgctgaa tctgctgcat ctctaaaagg tccaaagaaa cttgatgcat
     8281 acttgacacc ataactcatt ataggaatat tgtaatagcc ggcttcatct aatccacgac
     8341 gaatttcagc aacaaaacca tccatcatat tacttggcgc aataatatca gcaccagctt
     8401 ccacttgaga aattgctgtt ttaacaagta gtggcaatga tttatcattg tcaacgtcat
     8461 gtgtatggtc atcaatcacg ccacaatgac catgatcagt atattcacat aaacaagtgt
     8521 ctgcaacaat taataagtca tcatacattt ttttagcaat acgtgttgcc tgttgaataa
     8581 caccatcgtg aatgtatgca ccagtaccta tatcatcttt tgagtttgga acaccgaaaa
     8641 acataatggc acgtatgcct aagtcataag cttcttttaa ttcactttca agtaaattca
     8701 aactgatttg gtatacacct ggcaatgact taatttcttt tttcacatcg tctttttcaa
     8761 ctacaaaaat tggatatatt aaatcttctt ttcttacatg attctctcta accatatctc
     8821 tcattgtcgc tgatgatctc aatcttctat gtctatcaaa tttcattttt agcccctact
     8881 ttctaaaatc ttttcaatta gtgattcgag tgtttgaatt tctgcaattg ttactggttg
     8941 ttgatatgat ttaatggtcc gtgctgtttg ttctccaata gcaaaatacg acttgaattt
     9001 tggtacaaat ccttcattaa aataataacg tactgccgac gaacttgaaa atgttaatgc
     9061 atcgatttgt tgatgttcta tcatttcttt aacatcttgt atattttgtt tgttaggcac
     9121 tgaagtatat aaatctattt taacaacttc attatcttta gataacgctg ctaataacaa
     9181 tggtctcgcc aattcactcg aaggcaaaag tattttttgg ttagtttgat taaatgattt
     9241 taaaaatcct tcttgagaaa agtcgtttgg cataaaatca actcgaatgc caagtgattc
     9301 acaatattgc gctgtcttac ttcctatcac agcaatgtta tcaacattaa ttccttttag
     9361 atatttataa aagaatttca cagcattttt agatgaaaaa ataagccagt catagcgttg
     9421 atttaacaaa tgaatatcaa aatttagtgg ctttatatca ataaagggtt tgtgaataat
     9481 tgatactaaa tcactttgca tgtcatttgt ttgtgtcata actacaactg gcttcatatt
     9541 taaacgtcac tccattattt aatgttgttc atttaagcgt tttataattt cataagcacc
     9601 ttgctctttt aatttgttac tcactgtttt gcctaactca accggatctg ttccgttcat
     9661 tgtatattca aatcgttctt taccatctgg ggtcataatt aaacctgtaa attcgatttc
     9721 gttttgatct gagattgtag catatcctgc aattggcacc tgacaactac catccatttc
     9781 tgctaaaaac gttcgttcag cagtcacaca ttttgcaacc tcatcattat gtactttgct
     9841 taataatgtt aatagttctt catcgtcact acgacattct atccctaaag caccttgtcc
     9901 gattgcaggt aacaatgtat ctctatcaag ataagatgtt acaatatcat ctgaccagcc
     9961 cattcttctt aaaccagctg cagctaaaat aatcgcatca taatcttcag tttgtaactt
    10021 ttctaatcgt gtatctatat tacctctaat ccatttaatc tctaaattag gatacttaga
    10081 taatatttgt gcaccacgac gtaatgaact agtaccaata atactgcctt ctggcaattg
    10141 ggatagtggt gtatgtgttt tagaaatata cgcatcaaaa ggtaattctc tatcagggat
    10201 acaacctaat gttaaacctt ccggaattac acttggtacg tctttaagcg agtgtattgc
    10261 catatcgata tttttttcaa aaagttcatg ttgtatttct ttaacaaata agcctttgcc
    10321 tccgacttta gacaattgtt tatctactat acgatcgcct ttcgtgacaa tttctttaat
    10381 ttcaatttct agatttggct cgacagcttt taatttatca ataaattgct ggctttgtgt
    10441 taaagctaat ttacttcttc tggagccaac gactaattta cgcatgttca attcctccta
    10501 ggaacggatt gctctagatt attttctcaa ttcacaaaat gtgttgcaaa aaataaatta
    10561 atcatattta agcaaaataa aataatgtta tagtatatta aatatcttga attcaaccat
    10621 ttgttgattc taagtaaaat ataacttcca tataatactg taataattga agagagtatt
    10681 accttcgggt caatgaatat acgttcacca actgaaatta caccccactg tgtacctaaa
    10741 ataatactaa atatgagaat tatccaccca cttaacgttg agtaaaacac aattgattca
    10801 agtgtagcaa cgctaccaat tctaaagtat ttttgatcaa aacgtttttc cttcaaatta
    10861 cggtattgca tgatatacag taatgcattg acaaaagcta aggcaaagaa gacataactt
    10921 aacacagcta gaccgatatg gactaacagt aactcgtcta caacagcaat tttctgaacc
    10981 ttattagtat aatgtgtcgg ttgaaatgta ttcatcccta aaagtgttaa ccctattaaa
    11041 ttcaagaaaa acacagagaa attcaaaact ttgataaggt taagaattaa agagattgaa
    11101 ataatcagcc aacttaaagt atagaataca tctgaaatag accctaatgg aatatgtcta
    11161 gtttgtataa taaaaataga taaagagatt gtttgtaaaa cccaaacaat ccccaataaa
    11221 tatatgccta aacttctaat cttatgactt ttttgtacaa aatcataaaa atagcaaatg
    11281 atactgatta agtatattaa taatataatt tcattgaatc gaataaacag gttttcttgc
    11341 atattatcac catattaata tgcttattca aaactaaaga tacgtcgcgc tgaaatttcc
    11401 ttgaccttgc tttctttttg ttgcttcgct tgctcatgag gacattcagc ttcgatatca
    11461 aatatatttt gaaatagctc taatttttca ttacttttct tatcactact taattctttg
    11521 gcctgtttaa taggatcttt caacatttga ttgatgatac tttttgtatg tttagatata
    11581 attttacgtt ctctttcgct taaccctggc aatttacgat caatactgtc catcgtttct
    11641 gcttgaattg ccatagcttt ttcacgtaaa gctctaatca ctggaacaac acccaacata
    11701 ctaatccact cattgtgtgc atgtatttct gcaggaattt gttccgaaat tgttgcagcc
    11761 gctaattgtc gctcacgtaa gtttgcatca actaaacctt ttaagtcatc aacatcataa
    11821 ttaaagatgt ttgtgatggc actaatacca ggttcaatat ctcgaggaac tgcaatatca
    11881 atcaatacta gtgaatcttg ctttctattt tctgcaattc tttctatcat ttcatttgta
    11941 atgatataag attgtgcact cgttgaacta atcacaatat ctgcactttc aagtaaattt
    12001 ggtaatgatg atagttcatc atatttcact tgatgctttg ctgctaattt catagcattt
    12061 tcaattgttc tatttactac tgtaatatca gtaattccag aaccaagaag atttaatagt
    12121 gataattcac tcatttcccc tgcaccaata atgatagctt gcttactttt caatttgcca
    12181 aatacttttt tcgccaactc gaccgcagca taagacacac ttacagcatt atcagctata
    12241 tctgtttcat tatgtgctct ttttgcaaaa gtaattgcct gtttaaatag atgattaaaa
    12301 attgttcctg tcgtacctgt gctttgcgct aagaaaaatg catctcttat ttgacctaaa
    12361 atttgagttt ctccaagtac gattgaatct aaaccagaag tgacacgcaa taaatgttct
    12421 actgcttcgt cccccacttt tacttctgac attgctttaa tatcatctac ttcaaatcca
    12481 aatgcacgag ctagaaatcg ttgaatatag taacgacctg tgtgaatttg atcaacaaca
    12541 gcatatactt cagttcgatt acatgttgat aatatgacat tttctaaaat agatttagtt
    12601 tcatataaat cttcatgggc aattcgtaag gcatcatctc taaaagcaac ttgctctctt
    12661 agtgcaacat cagctgtgcg atgatttata ctaattgcaa taaaatgcat ttgaaacgcc
    12721 ccc
//
LOCUS       DAKTIO010000073        12662 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.073, whole genome shotgun sequence.
ACCESSION   DAKTIO010000073 DAKTIO010000000
VERSION     DAKTIO010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12662
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.073"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(32..199)
                     /locus_tag="O0293_001497"
     CDS             complement(32..199)
                     /locus_tag="O0293_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280385.1"
                     /translation="MKRIVILENVIVDNPIWLNHVDYLVKSVLLKSIKLRFQEVRKFE
                     ILVKIMNRIHI"
     gene            complement(327..830)
                     /locus_tag="O0293_001498"
     CDS             complement(327..830)
                     /locus_tag="O0293_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCY0280386.1"
                     /translation="MSKWIIVSDNHTESGVLYQIYEMHPDADVYLHLGDSEFAYDDTE
                     LSLFNRVKGNCDFYPEFENEAVAKYNDVKAFYTHGHLYQVNRTRDLLAEKGLELGCLF
                     AFYGHTHVAKYEYINGVHVINPGSISQSRSSMEETYAEVIIDDQTLHGTINFKNRHHE
                     TISHTTF"
     gene            complement(823..1410)
                     /locus_tag="O0293_001499"
     CDS             complement(823..1410)
                     /locus_tag="O0293_001499"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCY0280387.1"
                     /translation="MKEIVIASNNQGKINDFKVIFPDYHVIGISELIPDFDVEETGST
                     FEENAILKSEAAAKALNKTVIADDSGLEVFALNGEPGIYSARYAGENKSDEANIEKLL
                     NKLGNTTDRRAQFVCVISMSGPDMETKVFKGTVSGEIADGKYGENGFGYDPIFYVPKL
                     DKTMAQLSKEQKGQISHRRNAINLLQAFLEGDKNV"
     gene            complement(1422..2222)
                     /gene="racE"
                     /locus_tag="O0293_001500"
     CDS             complement(1422..2222)
                     /gene="racE"
                     /locus_tag="O0293_001500"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCY0280388.1"
                     /translation="MNKPIGVIDSGVGGLTVAKEIMRQLPNETIYYLGDIGRCPYGPR
                     PGEQVKQYTVEIARKLMEFDIKMLVIACNTATAVALEYLQKTLSIPVIGVIEPGARTA
                     IMTTRNQNVLVLGTEGTIKSEAYRTHIKRINPHVEVHGVACPGFVPLVEQMRYSDPTI
                     TSIVIHQTLKRWRNSESDTVILGCTHYPLLYKPIYDYFGGKKTVISSGLETAREVSAL
                     LTFSNEHASYTEHPDHRFFATGDPTHITNIIKEWLNLSVNVERISVND"
     gene            complement(2458..3273)
                     /gene="sdhB"
                     /locus_tag="O0293_001501"
     CDS             complement(2458..3273)
                     /gene="sdhB"
                     /locus_tag="O0293_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HCY0280389.1"
                     /translation="MTEQSVKNTPQHETQSKPKQKTVKLIIKRQDTSDSKPYEETFEI
                     PYRENLNVIACLMEIRRNPVNIKGEKTTPVVWDMNCLEEVCGACSMVINGRARQSCSA
                     IVDQLEQPIRLEPMNTFPVIRDLQVDRSRMFDNLKRMKAWIPIDGTYDLGPGPRMPEK
                     KRQTAYELSKCMTCGVCLEVCPNVTENNKFVGAQAISQVRLFNLHPTGSMTKDERLNA
                     LMGTGGLQQCGNSQNCVNACPKGIPLTTSIAAMNRETTFHMFKSFFGSDHEVE"
     gene            complement(3273..5039)
                     /gene="sdhA"
                     /locus_tag="O0293_001502"
     CDS             complement(3273..5039)
                     /gene="sdhA"
                     /locus_tag="O0293_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCY0280390.1"
                     /translation="MAEKHLIVVGGGLAGLMSTIKAAEKGAHVDLFSVVPVKRSHSVC
                     AQGGINGAVNTKGEGDSPWIHFDDTVYGGDFLANQPPVKAMTEAAPKIIHLLDRMGVM
                     FNRTNEGLLDFRRFGGTLHHRTAYAGATTGQQLLYALDEQVRAYEVDGLVTKYEGWEF
                     LGIVKGDDDSARGIVAQNMTTAEIETFGSDAVIMATGGPGIIFGKTTNSMINTGSAAS
                     IVYQQGAIYANGEFIQIHPTAIPGDDKLRLMSESARGEGGRIWTYKDGKPWYFLEEKY
                     PDYGNLVPRDIATREIFDVCINQKLGINGENMVYLDLSHKDPHELDVKLGGIIEIYEK
                     FTGDDPRKVPMKIFPAVHYSMGGLYVDYDQMTNIKGLFAAGECDFSQHGGNRLGANSL
                     LSAIYGGTVAGPNAIDYISNIDRSYTDMDESIFEKRKAEEQERFDKLLAMRGTENAYK
                     LHRELGEIMTANVTVVRENEKLLETDKKIVELMKRYEDIDMEDTQTWSNQAVFFTRQL
                     WNMLVLARVITIGAYNRNESRGAHYKPEFPERNDEEWLKTTMASFQGAFEKPQFTYDD
                     VDVSLIPPRKRDYTSKSKGGKK"
     gene            complement(5091..5705)
                     /locus_tag="O0293_001503"
     CDS             complement(5091..5705)
                     /locus_tag="O0293_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b558 subunit"
                     /protein_id="HCY0280391.1"
                     /translation="MAQSKNEFYLRRIHSLLGIIPIGAFLVVHLLVNHQATQGAEAFN
                     KASNFMESLPFLIIVEFLFIYIPLLYHGLFGIHIAFTAKENVGHYSIFRNWMFFFQRV
                     SGILTFIFIGIHLWQTRLQKAFYGKEVNYDLMHETLQHPGWAIFYIICIIAVVFHFAN
                     GLWSFLVTWGGLQSPKSQRVFTWVSLIVFLVISYIGVTAIIAFM"
     gene            complement(6029..7810)
                     /gene="uvrC"
                     /locus_tag="O0293_001504"
     CDS             complement(6029..7810)
                     /gene="uvrC"
                     /locus_tag="O0293_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCY0280392.1"
                     /translation="MEDYKQRIKNKLNVVPMEPGCYLMKDRNDQVIYVGKAKKLRNRL
                     RSYFTGAHDAKTTRLVGEIRRFEFIVTSSETESLLLELNLIKQYQPRYNILLKDDKSY
                     PFIKITKEKYPRLLVTRTVKQGTGKYFGPYPNAYSAQETKKLLDRIYPYRKCDKMPDK
                     LCLYYHIGQCLGPCVYDVDLSKYAQMTKEITDFLNGEDKTILKSLEERMLTASESLDF
                     ERAKEYRDLIQHIQNLTNKQKIMSSDKTIRDVFGYSVDKGWMCIQVFFIRQGNMIKRD
                     TTMIPLQQTEEEEFYTFIGQFYSLNQHILPKEVHVPRNLDKEMIQSVVDTKIVQPARG
                     PKKDMVDLAAHNAKVSLNNKFELISRDESRTIKAIEELGTQMGIQTPIRIEAFDNSNI
                     QGVDPVSAMVTFVDGKPDKKNYRKYKIKTVKGPDDYKSMREVVRRRYSRVLNEGLPLP
                     DLIIVDGGKGHMNGVIDVLQNELGLDIPVAGLQKNDKHQTSELLYGASAEIVPLKKNS
                     QAFYLLHRIQDEVHRFAITFHRQTRQKTGLKSILDDIDGIGNKRKTLLLRSFGSIKKM
                     KEATLEDFKNIGIPENVAKNLHEQLHK"
     gene            7976..8071
                     /locus_tag="O0293_001505"
     CDS             7976..8071
                     /locus_tag="O0293_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280393.1"
                     /translation="MLDYHLTFIDLLIPPFSSRKQYDDLSFNFLY"
     gene            complement(8134..8448)
                     /gene="trxA"
                     /locus_tag="O0293_001506"
     CDS             complement(8134..8448)
                     /gene="trxA"
                     /locus_tag="O0293_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCY0280394.1"
                     /translation="MAIVKVTDADFDSKVESGVQLVDFWATWCGPCKMIAPVLEELAA
                     DYEGKADILKLDVDENPSTAAKYEVMSIPTLIVFKDGQPVDKVVGFQPKENLAEVLDK
                     HL"
     gene            complement(8621..10969)
                     /locus_tag="O0293_001507"
     CDS             complement(8621..10969)
                     /locus_tag="O0293_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCY0280395.1"
                     /translation="MRQKTLDVLEFEKIKSLVANETISDLGLEKVNQMMPATNFETVV
                     FQMEETDEIAQIYNKHRLPSLSGLSKVSAFIHRADIGGVLNVSELNLIKRLIQVQNQF
                     KTFYNQLVEEDEGVKYPILDDKMNQLPVLTDLFQQINETCDTYDLYDNASYELQGIRS
                     KISSTNQRIRQNLDRIVKSQANQKKLSDAIVTVRNERNVIPVKAEYRQDFNGIVHDQS
                     ASGQTLYIEPSSVVEMNNQISRLRHDEAIEKERILTQLTGYVAADKDALLVAEQVMGQ
                     LDFLIAKARYSRSIKGTKPIFKEDRTVYLPKAYHPLLNRETVVANTIEFMEDIETVII
                     TGPNTGGKTVTLKTLGLIIVMAQSGLLIPTLDGSQLSVFKNVYCDIGDEQSIEQSLST
                     FSSHMTNIVEILKHADKHSLVLFDELGAGTDPSEGAALAMSILDHVRKIGSLVMATTH
                     YPELKAYSYNREGVMNASVEFDVDTLSPTYKLLMGVPGRSNAFDISKKLGLSLNIINK
                     AKTMIGTDEKEINEMIESLERNYKRVETQRLELDRLVKEAEQVHDDLSKQYQQFQNYE
                     KSLIEEAKEKANQKIKAATKEADDIIKDLRQLREQKGADVKEHELIDKKKRLDDHYEA
                     KSIKQNVQKQKYDKIVAGDEVKVLSYGQKGEVLEIVNDEEAIVQMGIIKMKLPIEDLE
                     KKQKEKVKPTKMVTRQNRQTIKTELDLRGYRYEDALIELDQYLDQAVLSNYEQVYIIH
                     GKGTGALQKGVQQHLKKHKSVSDFRGGMPSEGGFGVTVATLK"
     gene            complement(10979..>12662)
                     /gene="polX"
                     /locus_tag="O0293_001508"
     CDS             complement(10979..>12662)
                     /gene="polX"
                     /locus_tag="O0293_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HCY0280396.1"
                     /translation="EQIATYMELKGENTFKISAYRKAAQSLELDERPLDEISDVTELK
                     GIGKGVAEVINDYRETGESQYLQQLQEEVPEGLIPLLKIQGLGSKKIAKLYKELNIVD
                     KASLQVACENGKVSELSGFAKKTEQNILEAVKQLGAKKDRYPIDQMRRLNQEIIDYID
                     TLNYIDQYSSAGSFRRFKEMSKDLDFIISTDNPKAVQQQLLNIPNKVKEVAVGNTKVS
                     LELAYDDETIGVDFRLIEPSAFYHTLQHFTGSKEHNIRIRQLAKARDEKVSEYGIEQA
                     DGTLIQYDSEAKIYEHFNVNFIPPAMREDGSEFDKDLSNIITIDDINGDIHMHTTYSD
                     GAFSIRDMVEANIAKGYKFMVITDHSQSLRVANGLQVERLLRQNEEIKALDKEYSEID
                     IYSGTEMDILPDGSLDYDDEILAQLDYVIGAIHQSFNQSEEQIMERLANACRNPYVRH
                     IAHPTGRIIGRRDGYKPNIEQLMALAEETNTVLEINANPHRLDLSADIVRKYPNVKLT
                     INTDAHHTNHLDFMNYGVATAQKGFVTKDRVINALSREAFKDFIENNTKLKK"
BASE COUNT         3801 a         2470 c         1767 g         4624 t
ORIGIN      
        1 attgatataa tttatgactt tagaaatcct tttaaatatg tatacgattc atgattttaa
       61 ctaaaatttc aaatttcctg acttcttgga atcttaattt tatacttttt aataatacac
      121 ttttaaccaa ataatcaaca tgattcaacc atatagggtt atcaacaatc acattctcca
      181 aaattacaat tcttttcata acataaatgt gttattcatt ccaattatct tataaaattg
      241 tcagaaatac ttttaaacaa acattcatga cacgacctcc tagttaaatc agtctataaa
      301 ctaaaaaaag ccatagctac ttctatttaa aaagtagtat gactgattgt ttcgtgatgt
      361 cgatttttga aattgatggt gccatgtaaa gtttgatcat caataataac ttcagcatat
      421 gtttcttcca ttgaacttct agattgagat atacttccag gattaataac atgaacacca
      481 ttaatatact catattttgc cacatgtgta tgtccataaa atgcaaacaa acaacctaat
      541 tcaagtcctt tttcagctaa taaatctctt gttcgattga cttgatataa atgtccatga
      601 gtataaaatg ctttcacgtc attatatttt gcgaccgctt cattttcaaa ttctgggtaa
      661 aaatcacaat tgccttttac tctattaaat aagctaagtt ccgtatcatc atacgcgaat
      721 tctgaatctc ctaaatgtaa atatacatct gcatctgggt gcatttcata aatttgatat
      781 aaaacgcctg attcagtatg gttatcactc acaataatcc atttagacat ttttatcacc
      841 ttcaagaaaa gcttgaagta aattaatcgc atttcgtcta tgactaattt gccctttttg
      901 ttcttttgaa agttgagcca tggttttatc taatttcggt acataaaata tcggatcata
      961 tccgaaacca ttttcgccat attttccatc tgcaatttca cctgaaacag tacctttaaa
     1021 tacttttgtt tccatatcag ggccactcat acttatgaca caaacaaatt gcgcacgacg
     1081 atcagttgta ttaccaagct tatttaataa tttttcaata tttgcttcat cgcttttatt
     1141 ttcaccagca taacgtgcag agtatatacc tggctcacca tttaatgcaa aaacttctag
     1201 tccactgtca tcagctatga ccgttttatt caatgctttt gcagcagctt ctgattttaa
     1261 tatagcattt tcttcaaatg ttgatcctgt ttcttccaca tcaaaatctg gtattagttc
     1321 tgaaatacct attacgtggt aatctggaaa tattacttta aagtcattta ttttcccttg
     1381 attattcgat gcaataacaa tctctttcat taaaaatcct cctagtcatt cactgatata
     1441 cgttccacat tgacagataa atttaaccac tctttgataa tgttagtaat gtgagtagga
     1501 tcacctgttg caaaaaatcg atgatctgga tgttcagtat aacttgcatg ttcattacta
     1561 aatgttagca atgcactaac ttcacgagcc gtttctaatc cagacgaaat cactgtcttt
     1621 ttaccaccaa aataatcata gataggttta tagagcaatg gatagtgggt acatcctaaa
     1681 atgacagtat cagactcact attacgccaa cgtttcagtg tttgatgaat gacaatgctt
     1741 gtaattgttg gatcactata tctcatttgt tctacaagtg gcacaaaacc tggacaggca
     1801 acgccatgta cctctacatg tggattgata cgtttaatat gcgtacgata tgcttcagat
     1861 ttaattgtgc cttccgttcc tagtactaat acattttgat ttctagtagt cattattgct
     1921 gttctagcac ctggttcaat tacgccaatc actgggattg ataaggtctt ttgtaaatat
     1981 tctaaagcta cagcagttgc tgtattacaa gcaatcacga gcatttttat atcaaattcc
     2041 attaatttac gagcgatttc aactgtatat tgttttactt gttctcctgg tcttggccca
     2101 tatggacatc gtccaatatc acctaagtaa taaatcgtct catttggcaa ctgacgcata
     2161 atttctttag ctactgtcaa acctccgaca ccagagtcta ttacacctat tggtttattc
     2221 atattcggtc atccttactt ttaatttaat ttttagttta tcataactaa gcattggatt
     2281 ttagtattat gcactgtgtt taccattttt gtcattataa tatttatttt aaatcagccc
     2341 actatcatat tgtcatgtaa tctgcttatt aaaaaaatcc cttccaagtt attgtgtatc
     2401 tccattcaat ttaattttga aaggaacata acattttaac tcaaaaggga ttaattttta
     2461 ttctacttca tggtctgaac caaagaatga tttaaacatg tggaatgttg tttctctgtt
     2521 cattgctgca atggatgttg ttaatggaat acctttaggg caagcattaa cacagttttg
     2581 tgaattacca cactgctgta agccaccagt acccattaat gcatttaaac gttcatcttt
     2641 agtcatagat cctgttgggt gcaaattaaa caaacgaact tgcgagattg cttgtgcacc
     2701 aacgaattta ttattttcag taacattagg acaaacctct aaacatacac cacatgtcat
     2761 acatttagat aattcataag ctgtttgacg ttttttctct ggcatacgtg gtcccggacc
     2821 taaatcatac gttccatcaa ttgggatcca tgctttcata cgttttaagt tatcgaacat
     2881 tctagaacga tcaacttgta agtcacggat aactgggaaa gtattcattg gctctaaacg
     2941 aataggttgt tctaattgat caacaatcgc agaacaagat tgtcttgcac gaccattgat
     3001 aaccatagaa catgctccac atacttcttc taagcagttc atatcccaga caacaggtgt
     3061 tgttttttca cctttaatat taactgggtt acgtctaatt tccattaaac aagcaatgac
     3121 gtttaaattt tcacgatatg gaatttcaaa tgtttcttca taaggcttag aatcacttgt
     3181 atcttgtcgt ttaataatta attttactgt tttttgtttc ggtttagatt gtgtttcatg
     3241 ttgtggagtg tttttcactg attgttcagt cattattttt tacccccttt agacttactt
     3301 gtgtaatcac gtttacgagg tggtattaaa ctcacatcga cgtcatcata agtaaactgc
     3361 ggtttttcaa atgcgccttg gaatgaggcc attgtcgttt ttaaccactc ttcatcatta
     3421 cgctctggga attctggttt ataatgggca ccgcgtgatt cgttacggtt atatgcacca
     3481 atcgtaataa cacgtgcaag tactaacatg ttccatagtt gacgggtaaa gaataccgct
     3541 tggttactcc aagtttgagt atcttccata tcaatatctt cataacgttt catcaattca
     3601 acaatctttt tatctgtttc taacagtttt tcattttcac gaacaacagt tacatttgct
     3661 gtcataattt caccaagttc acggtgtaat ttatatgcat tttctgtacc gcgcatagct
     3721 aataatttat caaaacgttc ttgctcttca gctttacgct tttcaaaaat actttcgtcc
     3781 atatcagtat atgatcgatc aatatttgaa atataatcaa tcgcgtttgg acctgctact
     3841 gtaccaccat aaatcgctga taacaatgaa ttggcaccta agcggttacc accatgttga
     3901 gagaagtcac attctccagc tgcaaataac cctttaatat ttgtcatttg atcataatct
     3961 acatatagac cacccattga atagtgaaca gctgggaaaa tcttcattgg tactttgcgt
     4021 gggtcatcac cagtgaattt ttcataaatc tcaatgatac cacctagttt tacatctaac
     4081 tcatgtggat ctttatgtga caaatcaaga tataccatgt tttcgccatt tatacctaat
     4141 ttttggttaa tacatacatc gaaaatttca cgcgttgcga tatcacgagg tactaagtta
     4201 ccataatcag gatatttctc ttctaagaag taccaaggct taccatcttt atatgtccaa
     4261 attcgtccac cttcaccacg tgctgattca ctcattagtc gcagtttatc atcaccaggg
     4321 attgcagtag gatgaatttg aatgaactca ccattagcat aaatagcgcc ttgttggtaa
     4381 acaatggaag ccgctgatcc tgtattaatc attgagtttg ttgttttacc gaaaataata
     4441 ccagggccac ccgttgccat aataactgca tctgaaccaa atgtttcaat ctcagcagtt
     4501 gtcatatttt gtgcaacgat acctcttgca ctatcatcgt cacctttaac tatgccaagg
     4561 aattcccatc cttcatactt cgtaactaat ccatctactt catatgcacg aacttgttca
     4621 tccaatgcat ataataattg ttgtccagtt gttgcccctg catatgctgt tctgtgatgt
     4681 aatgtaccac cgaaacgtct aaaatctaat agaccttcat ttgttctatt gaacattacg
     4741 cccatacggt ctaataaatg aataatttta ggtgctgcct ctgtcatcgc tttaacaggt
     4801 ggttggtttg caaggaaatc gccaccatac actgtatcat caaagtgaat ccaaggagaa
     4861 tcgccttccc ctttagtatt gaccgcacca ttaatgccac cttgggcaca aacagagtgc
     4921 gaacgcttta ctggtacaac tgagaacaaa tctacatgtg cacctttttc tgccgcttta
     4981 attgttgaca ttaagcccgc taggccacct ccgacaacaa taagatgttt ctctgccata
     5041 aaaatttcac tcccctaaat tttcaatcta tatttgttaa atgcgatgta ttacataaag
     5101 gcaataattg cagtaacacc aatatacgaa ataactaaaa atacgattaa tgaaacccat
     5161 gtaaatactc gttgtgattt tggagattga agtccacccc aagtaactaa gaatgaccat
     5221 aagccatttg caaagtggaa cacaacagca ataatacaaa taatataaaa tattgcccat
     5281 ccaggatgtt gcaatgtttc gtgcattaaa tcgtaattca cttctttgcc gtaaaatgct
     5341 ttttgtaaac gtgtttgcca taaatggata ccaataaaga taaatgttaa gataccactc
     5401 actctttgga agaagaacat ccagtttcta aaaatcgagt aatgtccaac attttctttt
     5461 gctgtaaatg caatgtgtat accaaacaaa ccgtgatata acaacggaat gtatataaat
     5521 aaaaattcta caataattag aaatggtaat gattccataa agttagatgc cttattaaac
     5581 gcttcagcac cttgtgttgc ttggtgattc actaataaat gaacgaccaa aaatgcacct
     5641 attgggataa tacctaataa cgagtgaata cgtcttagat aaaattcatt ttttgattga
     5701 gccaaaagga gtcccccctg tgaacgaata tttaatttat tgagctattt atattaaacg
     5761 tacgcttaac cccctaaagt gatatcgttt ctagcgttgt cattatcatt aagcgaaaca
     5821 ttttaaagac aaatacacac tgcacgatca ccaaactgca tgtcgaacaa tgtaacattt
     5881 ggattcgata tttaaaattg cttgtgatga taaactttct catttagaaa acgcttccac
     5941 gtacattcaa aaaaataact ttgttaacca tattgtaaca ttatttcata tattttgggg
     6001 catgagaatg attctcacgc ccagtaattt atttatgcaa ttgttcatgt aggttctttg
     6061 cgacgttttc aggaatacct atatttttaa aatcttcaag tgtagcttcc ttcattttct
     6121 tgattgaacc gaatgaacgc aataataatg ttttacgttt gttaccgata ccatctatat
     6181 catcaagtat tgatttcaag cctgtctttt gacgtgtttg tctatgaaat gtgattgcga
     6241 atctgtgaac ctcatcttgg atacggtgca acaaataaaa tgcctggcta tttttcttca
     6301 gtggtacaat ttctgcacta gcgccatata ataattcaga tgtttggtgt ttatcatttt
     6361 tctgcaaacc tgcaacaggg atatcaagac ctaattcgtt ttgtagcaca tcaataaccc
     6421 cgttcatatg tcctttacca ccatctacta ttattaaatc aggtaatggt aatccttcgt
     6481 ttaaaacgcg agaatatcgt cgtcttacta cttctctcat tgatttgtaa tcatctggac
     6541 ctttaaccgt tttgatttta tactttctat aatttttctt atctggttta ccgtcgacaa
     6601 atgtaaccat tgctgacact ggatccacac cttgaatatt agaattatcg aatgcttcaa
     6661 ttctaattgg tgtttgaatt cccatttgtg ttccaagttc ttcaatagct ttaatcgttc
     6721 tggactcatc acgtgatatt aattcaaatt tattatttaa ggatacttta gcgttatgtg
     6781 cagctaggtc aaccatatct tttttgggac ctcgcgcggg ttgaacgatt ttagtgtcca
     6841 caacagattg aatcatttct ttatccaaat tacgtggtac atgaacttcc ttaggtaaaa
     6901 tatgttggtt taagctataa aattgtccaa taaatgtata aaattcttct tcttctgttt
     6961 gctgtaatgg aatcatcgtt gtatctcgct ttatcatatt accttgtcgt ataaagaaaa
     7021 cttggataca catccatcct ttatcaacac tataaccaaa gacatcacga atcgttttat
     7081 ctgatgacat aattttttgt ttgtttgtca gattttgaat atgttgaatt aaatctctat
     7141 attctttagc ccgttcaaaa tcaagtgatt cacttgcagt taacattcgc tcttctaaac
     7201 tttttaaaat tgttttgtct tccccattca gaaaatcagt aatttccttc gtcatttgtg
     7261 cgtatttact caaatcaacg tcatatacac atggtcctaa acattgtcca atatggtaat
     7321 aaagacataa tttatctggc atcttatcac atttgcgata tggatatatt ctgtctaata
     7381 actttttagt ttcttgagca gaatatgcat tcggatacgg tccgaaatat ttgccagtac
     7441 cttgttttac agttctcgtc actagtagtc taggatattt ctccttcgta attttaataa
     7501 atggataact tttatcatcc tttaataata tattatatct tggttgatat tgtttaatca
     7561 gattcaattc cagtaaaagt gattctgttt cacttgacgt gacaataaac tcaaagcgac
     7621 gtatttcacc aaccagtctc gttgttttag catcatgagc acccgtaaaa tatgatcgca
     7681 atcgatttct tagcttttta gctttgccaa catatatcac ttgatcatta cgatctttca
     7741 ttaaatagca tcctggttcc ataggtacga catttaattt atttttaatt cgttgcttat
     7801 agtcttccaa aaatatcccc ccttctaatt ttaaaatgaa tgatgctaca aaagtgcttc
     7861 aaatttatca ccactaattc tagttaataa tcaacatctt cgagtatgac ttttcatgca
     7921 catcatgtaa tattgaacat ggttttatca aaccaactgc tacaaatcta tcagcatgct
     7981 agattatcat ttaacgttca tagatttatt aatcccacca ttctcaagtc gtaaacaata
     8041 tgatgactta tcattcaact ttttatatta aaaacacaaa caaaaactgg agcaattcat
     8101 taaagaaatg ccccagtcgt cattggttgt aacttataaa tgtttatcta aaacttcagc
     8161 taagttttct tttggttgga aaccaacaac tttatcaact ggttgaccgt ctttaaagac
     8221 gattaatgtt ggaatactca tcacttcata tttagctgca gttgatggat tttcatcaac
     8281 atctaatttt aaaatgtcag ctttaccttc atagtcagct gctaattctt ctaataccgg
     8341 agcgatcatt ttacatggac cacaccatgt tgcccaaaaa tctactagtt gtacaccaga
     8401 ttctactttt gaatcaaaat ctgcatctgt tacttttacg attgccataa atgccaatcc
     8461 tccttaatta ttattatgat gttcaaatta tatcagataa catgcttcat gtatttctat
     8521 ttgctcaatt tttatgctta tttcaaataa catgtttgaa atgtattctt gttgctttac
     8581 attattttaa ctgcagctat ttaatttatc aaattataat ttattttagt gttgcaacgg
     8641 taacgccaaa tccaccttcg cttggcatac cacctctaaa gtcactaaca cttttatgct
     8701 ttttcaaatg ttgttgtaca cctttttgaa gtgcacctgt acctttacca tgaatgatat
     8761 aaacttgttc gtaattactt aaaacggctt gatctaaata ttgatctagt tcaattaaag
     8821 catcctcata acgatagcct cgtaagtcaa gttcagtttt aattgtttga cgattttgac
     8881 gtgtaaccat tttcgttggc ttaacttttt ctttttgttt tttctctaaa tcttcaatag
     8941 gtaacttcat tttaataatt cccatttgaa caattgcttc ttcatcattg acaatttcta
     9001 aaacttcacc cttttgaccg taagataata cttttacttc atcaccagca acaattttat
     9061 cgtatttttg cttttgtaca ttttgcttta tagatttcgc ttcataatga tcatctaatc
     9121 gtttcttctt atcaatcaat tcatgttctt taacatctgc acctttttgt tcacgcaatt
     9181 gtcttaagtc tttaataata tcgtcagctt cttttgttgc agccttaatc ttctgatttg
     9241 ctttttcttt cgcttcctct attagagact tttcataatt ttggaattgt tgatactgct
     9301 tagataaatc atcatgcact tgctccgctt cttttacaag acggtccagt tctaacctct
     9361 gtgtctctac acgtttgtaa ttacgctcta atgattcaat catttcattt atttcttttt
     9421 catcagtacc aatcatcgtc ttagccttat taataatatt caaactaaga cctaactttt
     9481 tagaaatgtc aaaagcattt gaacgacccg gcacacccat taataactta tacgttggac
     9541 tcaaagtatc tacatcaaat tctacactcg cattcataac gccttctcga ttataactat
     9601 atgctttaag ttcaggatag tgcgtcgttg ccattactag agaaccaatt tttctaacat
     9661 gatctaaaat gctcattgct aatgcagcac cttcactcgg atctgtacct gcacctaatt
     9721 catcaaataa aactaaacta tgtttgtctg catgctttaa aatttcaact atattcgtca
     9781 tatgagatga aaaagttgat aatgattgtt ctattgattg ttcatctccg atatcgcaat
     9841 atacattttt aaatacactt aactgactac catcaagtgt gggaatcaac aatcctgatt
     9901 gagccataac aataattaaa cctaatgttt ttaatgttac agttttacca cctgtattcg
     9961 gtcctgtaat aattaccgtt tcaatatctt ccataaattc gatggtatta gctacaacag
    10021 tctcacgatt taataatgga tggtatgctt taggtaaata tacagtacgg tcctctttaa
    10081 atatcggctt tgttccttta atacttctac tatatctcgc ttttgcgatt aaaaaatcta
    10141 actgacccat gacttgttct gccacaagta gtgcatcttt gtccgcagcc acataaccag
    10201 ttagttgcgt taaaatgcgt tctttttcaa ttgcttcgtc atgacgtaat cgactaattt
    10261 gattattcat ttcaacaact gatgatggct caatatacaa tgtttgtcct gaagcagatt
    10321 gatcatgtac aatcccatta aaatcttgtc gatattcagc tttgacaggt ataacgtttc
    10381 tttcattcct aactgttaca atagcatctg ataatttttt ctgatttgct tggcttttaa
    10441 caatacggtc caaattttgt ctaatacgtt gattcgtgct agaaatttta cttctaatcc
    10501 cttgcaattc ataactcgca ttatcatata aatcatacgt atcgcatgtt tcatttattt
    10561 gttgaaaaag atcagttaac acaggtaatt gattcatctt gtcatctaat attgggtatt
    10621 taacaccttc atcttcttca accaattgat tataaaatgt cttgaattga ttttgtactt
    10681 gaattaatct ttttatcaag ttaagctctg atacatttaa aacgccgcca atatcagcgc
    10741 gatgaatgaa tgctgatact ttagataagc cactcaagct tggtaaacga tgcttattat
    10801 agatttgagc aatctcatcc gtttcttcca tttgaaaaac aaccgtttca aaattagtag
    10861 ctggcatcat ttgattgacc ttttccaagc ctaagtcact aatagtttca ttggcaacga
    10921 gtgattttat tttttcaaat tctaagacgt ctaatgtttt ttgtctcata aaatccctct
    10981 atttcttaag ttttgtatta ttttcaataa agtctttaaa agcttcacgc gataatgcgt
    11041 taatcactct atcttttgtt acaaatcctt tttgcgcagt tgctacgcca taattcataa
    11101 aatctaaatg atttgtatga tgcgcatcag tgttaatagt taatttcaca tttggatatt
    11161 tacgaacgat atcagcgctc agatccagtc gatgtggatt ggcattaatt tctaatactg
    11221 tattcgtttc ttcagctaat gccattaatt gttcaatatt cggtttataa ccatctcttc
    11281 tacctataat acgccctgtt ggatgcgcta tatgtcgcac gtatggattg cgacatgcat
    11341 tagctaatcg ttccataatt tgttcttctg attggttaaa gctttgatga atagctccaa
    11401 ttacataatc aagttgtgct aaaatttcat catcataatc cagcgagcca tcaggtaata
    11461 tatccatttc tgtacctgaa taaatatcaa tttcactata ttctttatct aaagccttaa
    11521 tttcttcgtt ttgtcttaaa agtctttcta cttgtaagcc attagcaaca cgtaaacttt
    11581 gtgaatgatc agtaattacc atgaatttat aaccttttgc gatatttgct tctaccatgt
    11641 ctcgaataga aaacgcacca tcactatacg ttgtatgcat atgaatatca ccattaatat
    11701 catctattgt aatgatatta cttagatctt tatcaaattc gctaccatct tctcgcatag
    11761 caggtggtat aaaattcaca ttaaaatgtt catatatctt ggcttcacta tcatattgaa
    11821 ttaatgtacc atcagcttgt tcaattccat attcacttac tttttcatca cgtgctttag
    11881 caagttgtcg aattcttata ttatgttctt ttgacccagt aaaatgctgc aatgtatgat
    11941 aaaaagcact tggttcaatt aatcgaaaat cgacaccaat cgtttcatca tcatacgcta
    12001 attctaatga aacttttgtg ttccccactg caacttcttt tactttattg ggaatattta
    12061 ataattgctg ctgcactgct tttgggttat cggtacttat tatgaaatct aaatctttgc
    12121 tcatttcttt aaaacgacgg aagcttcctg cagatgaata ttgatcgata taatttaatg
    12181 tatctatata atcaatgatt tcttgattaa gtcttctcat ttgatcaatt ggatatctat
    12241 ctttcttagc accaagttgt ttcacagctt ctaatatgtt ttgttccgtt ttcttagcaa
    12301 atccgcttaa ttcactaact tttccatttt cacaagcaac ttgaagtgac gctttatcaa
    12361 caatattcaa ctctttatat agcttagcaa ttttcttgct tccaagtcct tgaattttca
    12421 aaagtggaat aagaccttcc ggaacttctt cctgtaattg ctgtaaatac tgagattcac
    12481 cggtctcacg gtaatcattg attacttctg caacaccttt accaatgcct tttaactccg
    12541 ttacatcaga tatttcatct aatggtcgtt catctaattc aagactttga gctgcttttc
    12601 gatacgctga tattttaaaa gtattttccc cttttaattc catataagta gcaatttgtt
    12661 ct
//
LOCUS       DAKTIO010000074        12467 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.074, whole genome shotgun sequence.
ACCESSION   DAKTIO010000074 DAKTIO010000000
VERSION     DAKTIO010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12467
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.074"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..392
                     /locus_tag="O0293_001509"
     CDS             <1..392
                     /locus_tag="O0293_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCY0280397.1"
                     /translation="LLNLQKRLNQTKKDIDYILSPIVNRPSLLDRLNKNGKTTDLNKL
                     ANLMNQGSDLLDSIPDIPTPKPEKTLTLGKGNGLLSGLLNADGNVSLPKAGETIKEHW
                     LPISVIVGAMGVLMIWLSRRNKLKNKA"
     gene            560..1120
                     /locus_tag="O0293_001510"
     CDS             560..1120
                     /locus_tag="O0293_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCY0280398.1"
                     /translation="MSRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPV
                     IFIRLVLDKHFNDVSSSNKVFSTIKAQGYAITEADASTRILEDLAPLEDEPIISKRRF
                     SAFTGSYLEVYLRANDINHLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDD
                     KHDFIIEQILSRSCDIESVESWKSSL"
     gene            complement(1387..3246)
                     /locus_tag="O0293_001511"
     CDS             complement(1387..3246)
                     /locus_tag="O0293_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HCY0280399.1"
                     /translation="MPKNKILIYLLSTTLVLPTLVSPTAYADTPQKDTTAKTTSHDSK
                     KSNDDETSKDTTSKDIDKADKNNTSNQDNNDKKFKTIDDSTSDSNNIIDFIYKNLPQT
                     NINQLLTKNKYDDNYSLTTLIQNLFNLNSDISDYEQPRNGEKSTNDSNKNSDNSIKND
                     TDTQSSKQDKADNQKAPKSNNTKPSTSNKQPNSPKPTQPNQSNSQPASDDKANQKSSS
                     KDNQSMSDSALDSILDQYSEDAKKAQKDYASQSKKDKNEKSNTKNPQLPTQDELKHKS
                     KPAQSFNNDVNQKDTRATSLFETDPSISNNDDSGQFNVVDSKDTRQFVKSIAKDAHRI
                     GQDNDIYASVMIAQAILESDSGRSALAKSPNHNLFGIKGAFEGNSVPFNTLEADGNQL
                     YSINAGFRKYPSTKESLKDYSDLIKNGIDGNRTIYKPTWKSEADSYKDATSHLSKTYA
                     TDPNYAKKLNSIIKHYQLTQFDDERMPDLDKYERSIKDYDDSSDEFKPFREVSDSMPY
                     PHGQCTWYVYNRMKQFGTSISGDLGDAHNWNNRAQYRDYQVSHTPKRHAAVVFEAGQF
                     GADQHYGHVAFVEKVNSDGSIVISESNVKGLGIISHRTINAAAAEELSYITGK"
     gene            3457..6438
                     /locus_tag="O0293_001512"
     CDS             3457..6438
                     /locus_tag="O0293_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCY0280400.1"
                     /translation="MKNAFKLFKMDLKKVAKTPAVWIILAGLAILPSFYAWFNLWAMW
                     DPYGNTGHIKVAVVNEDKGDTIRGKKVNVGNTMVNTLKKNKSFDWQFVSREKADHEIK
                     MGKYFAGIYIPSKFTHEITGTLRKQPQKADVEFKVNQKINAVASKLTDTGSSVVVEKA
                     NEQFNKTVTRALLEEANKAGLTIEENVPTINKIKNAVYSADKALPKINDFANKIVYLN
                     NHQADLDKYANDFRKLGNYKGDILDAQKKLNEVNGAIPQLNEKAKLILALNNYMPKIE
                     KALNFAADDVPAQFPKINQGLNIASQGIDQANGQLNDAKGFVTQVRSRVGDYQDAIRR
                     AQDLNRRNQQQIPQNSAANNETSNSAPAAGNGVASTPPSAPSGDTAPNNNVTQNTAPN
                     SNNAPVSTTPQSTSGKKDGQSFVDITTTQVSTANENTQNITDKDVKSMEAALTGSLLS
                     LSNNLDTQAKAAQKDSQALRNISYGILASDKPSDFRESLDNVKSGLEYTTQYNQQFID
                     TLKEIEKNENVDLSKEIDKVKAANNRINESLRLVNQLSNALKNGSSGTAEATKLLDQL
                     SKLDSSLSSFRDYVKKDLNSSLVSISQRIMDELNKGQTALSNVQSKLNTIDQVINSGQ
                     AILKNGKTRIDRLQTVLPSIEQQYISAVKNAQANFSKVKSDVAKAANFVRNDLPQLEQ
                     RLTNATASVNKNLPTLLNGYDQAVGLLNKNQPQAKKALSDLADFSQNKLPDVEKDLKK
                     ANKIFKKLDKDDAVDKLIDTLKNDLKKQAGIIANPINKKTVDVFPVKDYGSGMTPFYT
                     ALSVWVGALLMVSLLTVDNKHKSLESVLTTRQVFLGKAGFFIMLGMLQALIVSVGDLL
                     ILKAGVESPVLFVLITIFCSIIFNSIVYTCVSLLGNPGKAIAIVLLVLQIAGGGGTFP
                     IQTTPQFFQNISPYLPFTYAIDSLRETVGGIVPEILITKLIILTLFGIGFFVVGLILK
                     PVTDPLMKRVSEKVDQSNVTE"
     gene            complement(6550..7488)
                     /gene="manA"
                     /locus_tag="O0293_001513"
     CDS             complement(6550..7488)
                     /gene="manA"
                     /locus_tag="O0293_001513"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HCY0280401.1"
                     /translation="MALFLQPVFKDRIWGGHALKAFNYDIPNETTGECWAISAHPNGP
                     NTIINGPYKDMTLDQLWSQHRELFDNDSRDSFPLLTKVLDANDKLSVQVHPDDDYALK
                     HEGELGKTECWYILDAAPGAEIIYGVHAHNKQTLIDMIDNHEFDSLFKRIPVKPGDFY
                     YVPAGTVHAIGSGILILETQQSSDTTYRIYDYDRRDQNGQLRDLHLEQSKDVIELGNH
                     EPNTTPITTHIDTHTVTQFVDNQYFAVYKWDIHGDLKFNKPHAYCLVTIIDGTGTLTV
                     NDKHYDIQKGSSFILTTEDEDIHFVGNISAIVSHPG"
     gene            complement(7502..9454)
                     /locus_tag="O0293_001514"
     CDS             complement(7502..9454)
                     /locus_tag="O0293_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HCY0280402.1"
                     /translation="MKIVAITSCPNGIAHTYMAQEKLEQVAKEMGVDIKVETQGGVGA
                     ENVLTTQDIEEADGVIIAADKQVDLSRFVGKRLINENVREGIHNPRGLIQRIINQDAP
                     IYQSETNYHSKDRGKSKSGIQMVYQHLMNGVSFMVPFIVVGGLLIAIALTLGGETTSK
                     GLVIPDDSFWKSIENIGSLAFKFMVPILAGYIAVSIADKPGLVPGMIGGAIAADGSFY
                     GSDAGAGFLGGIVAGFLAGYIAKWIKDIKVPKAMAPIMPIIIIPIISSVVVGLIFIFL
                     IGAPISNIFEALTTWLKSMQGANIIILAMIIGAMIAFDMGGPVNKVAFLFGSALIAEG
                     NYAVMGMVAVAVCTPPIGLGLATFVQKYKFNHSEREMGKASFTMGLFGITEGAIPFAA
                     QDPLRIIPANIIGAMIASVIAAIGGVGDRVAHGGPIVAVLGGIDHVLWFIFAVIVGSL
                     VTMTTVLLLKRNTPVIAVDAPAQHTQLHDTDITQHDTEVDNVDNTAKHSHLNKPSHVF
                     DQQTMIITDHDMSRNEAIDMLIHKLKICRYVEHTSHLKNAILEREMGSTTAIGMNVAI
                     PHAKSDVVKQPIVAVMKNNHGVKWDSLDGSLPQLIFLIAVPNNSQDTHLKILQRLSKA
                     LMNDETRQSLINANSTTEIYNLLMKI"
     gene            complement(9531..11405)
                     /locus_tag="O0293_001515"
     CDS             complement(9531..11405)
                     /locus_tag="O0293_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCY0280403.1"
                     /translation="MLDRHLKLLQFFIKNPSKHISSNEIAKHVNVSNRTVRNDIHVIN
                     SNFMDDIIVSIKSKGYQLNTSQYTLETITERYTHIQSYKEKLLLSMAYQLLMHNKSQT
                     LQQLEQDYLLSKTVLNDYFVRIQQWCQKFNIALTIKKKQGIVVDGTDNDITNAIIHLN
                     QLSSGHVHVEDLILNELPDSHQRMISHIIQETLQQHSIETTDIQIQQLLIHLILIIKR
                     KQSLSELDTLNHDSKLISQSCIKQINERLGYDLNEKVINMFSFFIAYHFNKLDIGIER
                     IFIQSYIDRLIELMQQRIHVPFNEDSILQQNLYNHFSKAYLRITQNIYLNNPLVIEIK
                     KLYPFVFNTLFEAIDKLAIDTDIEMSEDEIAFLTIHFQAAIERRTKTQLNVVIACYYG
                     LGVSNFLETKINNLSEELSVINTIKLENITHYHFDNVDLLITTHDIPKQTLNILPKHL
                     TTIKVAPLFSEDDRHKIRHVVKQKQNPVQAHHHMDTVNFLVVNTEQKSRHTVQIFEEA
                     QKILQAHHAIVEGYIESALEREKSSSTYIGNFMAIPHGDPEKVLQSHVLIFRTKDVFP
                     WRQHDVKLVFFLAISHKDKAFTKQMMQLIANLDDDSVNHLCSLDDHSLKQQLFEYLQE
                     "
     gene            complement(11645..12103)
                     /locus_tag="O0293_001516"
     CDS             complement(11645..12103)
                     /locus_tag="O0293_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280404.1"
                     /translation="MKKLLITIVIIVLIGALGFAIYAAINHSTSSSNNQEQENKSTHK
                     KTETEENDQQDDSADKTDKNQNNGNNVTNDNQPSSPSNVPNNHSTVPSNTQPAQPKDS
                     NSNKNNTHGGGSQSSTNNQNNQQQNTPPANKSNNTNTPSKQTPQAQSPKN"
BASE COUNT         4088 a         1931 c         2265 g         4183 t
ORIGIN      
        1 atttattgaa tttacaaaag agactcaatc aaacgaaaaa agatatagat tatattttat
       61 caccaatagt aaatcgtcca agtttactag atcgattgaa taaaaatggg aaaacgacag
      121 atttaaataa gttagcaaat ttaatgaatc aaggatcaga tttattagac agtattccag
      181 atatacccac accaaagcca gaaaagacgt taacacttgg taaaggtaat ggattgttaa
      241 gtggattatt aaatgctgat ggtaatgtat ctttgcctaa agcgggggaa acgataaaag
      301 aacattggtt gccgatatct gtaattgttg gtgcaatggg tgtactaatg atttggttat
      361 cacgacgcaa taagttgaaa aataaagcat aattatattg ggggaagagc atctatatat
      421 ttttttaagt atataagacg tcttatttcc ccttaattta ttgtgaagta tatgcaaaat
      481 gcaatgaata gattgtccat cattttaacg ttataatgaa tttaacgact tagaactaca
      541 caagtaaagg agaatgaaga tgtctcgaaa aacggcgcta ttagttttgg atatgcaaga
      601 aggtatagcg agtagtgtac ctagaataaa aaatattatt aaagcgaatc agagagcaat
      661 tgaagcagca agacaacatc gaataccagt cattttcata cgtttagtgt tagataagca
      721 ttttaatgat gtctcctcga gtaataaagt gttttcaaca attaaagctc aaggatatgc
      781 gattactgaa gcagatgcat ctacacgaat acttgaagat ttagcaccac tagaagatga
      841 gccgattatt tctaagcgac gctttagcgc atttacaggt agttacttgg aagtttattt
      901 acgtgcaaat gatattaatc atttagtatt aacgggtgtc tctacaagtg gagctgtatt
      961 gagcacggca ttagaaagtg tagataaaga ctattatatt actgttttag aagatgctgt
     1021 tggtgataga tcagatgata aacatgactt tattattgaa caaattttat cacgctcatg
     1081 tgacattgaa tccgtagagt catggaaaag tagtttatag ttaatataac gtcaattaaa
     1141 gctcggcagt aatgtttgag aataagtaca tttgctcata tttataaaat gtgtgagatg
     1201 gcaattgaaa cggatatgat gaggaacatt tgaacataaa ataatatatt tatataaaac
     1261 gacccgaggc gttcgaactg aatgcctcgg gtttaattga ataagaaatc agacttatga
     1321 acagaaatat gtttaagtcc gaactccttg tttatactta taaattttac gggtttaata
     1381 taatacttat ttacctgtaa tatatgataa ttcttcagcg gcagctgcgt tgatagttct
     1441 atgagaaatg atacctaatc ctttaacatt ggattctgaa ataacgatag aaccatcact
     1501 gttaactttt tcaacaaatg ctacatgacc gtaatgttga tctgcaccaa attgtccagc
     1561 ctcaaataca acagcagcat gacgttttgg tgtatgactt acttgataat cacggtattg
     1621 agctcgatta ttccaattat gtgcatcacc taaatcacct gagatagatg taccaaattg
     1681 tttcatacgg ttatatacgt accaagtaca ttggccatgt ggatatggca tactatcaga
     1741 tacctcacgg aaaggtttga attcatctga tgaatcatca taatccttga tagaacgttc
     1801 atatttatct aaatctggca tgcgttcatc gtcaaactga gttaattgat agtgtttaat
     1861 aatactgttt aatttcttag catagtttgg atctgtagca tatgttttag ataagtgtga
     1921 tgttgcatct ttataagaat cggcttccga tttccatgtt ggtttataaa ttgttcgatt
     1981 gccatcaata ccatttttaa taaggtcaga gtaatctttt agtgattctt tcgtgcttgg
     2041 atattttcgg aatccagcat taatactata caattgatta ccatcagctt ctaatgtgtt
     2101 aaaaggaaca gaattccctt caaaagcacc tttgataccg aataaattat ggtttggtga
     2161 cttagctaaa gcactacgac ctgagtcaga ttctaagatt gcttgggcaa tcatgacaga
     2221 cgcataaata tcgttatctt gaccaatgcg atgtgcatct ttagcaattg atttgacaaa
     2281 ttgacgtgta tcttttgagt caacaacgtt aaattgtccg ctatcatcat tgttagatat
     2341 actaggatct gtttcgaata atgatgttgc acgtgtatcc ttttgattaa catcgttatt
     2401 gaatgattga gcaggtttag atttatgttt caattcatct tgtgttggta actgtggatt
     2461 ctttgtatta gatttttcat ttttgtcttt tttagattga gatgcataat ctttttgtgc
     2521 tttctttgca tcttcactgt attgatccaa aatagagtct aaagccgaat ctgacattga
     2581 ttgattatct ttcgatgaag atttttgatt tgctttatcg tcacttgctg gttgactatt
     2641 tgattgatta ggttgtgttg gctttggcga atttggttgc ttattagatg tacttggttt
     2701 tgtattgttt gatttaggtg ctttttgatt gtctgcttta tcttgtttag atgattgcgt
     2761 atcagtgtca tttttgatgc tattgtcact gtttttattc gaatcatttg ttgacttttc
     2821 gccattacga ggttgttcgt aatcagaaat atccgaattt aaattgaata agttttggat
     2881 taaagttgtt aatgagtaat tatcatcgta tttatttttg gttagcaatt ggtttatatt
     2941 ggtttgtggt aaattcttat aaataaaatc aatgatattg ttagagtctg aagtgctgtc
     3001 gtctatagtt ttaaattttt tgtcgttatt gtcttggtta cttgtattat ttttgtctgc
     3061 tttatcaata tctttacttg tagtatcctt agaagtttca tcgtcattag atttttttga
     3121 atcatgagat gttgtcttag ctgtagtatc tttttgaggt gtatcagcat aagcggtagg
     3181 tgaaactaaa gtaggtaata cgagcgtagt tgatagcaaa taaattaaaa ttttattttt
     3241 aggcatattt cgtattctcc cttgaaaaat ataataatta agtgtgataa taaactatga
     3301 tttgttataa tttatcgtat gctgaaaata gttgataggt atcaatcgac taaatatctt
     3361 ccagtaaatt gattatacta attcacaacg caaaaataaa ttaatttaca aaaaatatat
     3421 aaaaaatatg aataattcct acataggagt gtgacaatga agaacgcatt taaattattt
     3481 aaaatggatc tgaagaaagt agctaagacg ccagctgtgt ggattatctt agcaggctta
     3541 gctattttgc catcgttcta cgcttggttt aacttatggg caatgtggga tccatatggc
     3601 aacacgggac acatcaaggt cgcagtcgtt aatgaagata aaggcgacac aatcagaggg
     3661 aaaaaagtta atgtcggtaa tacgatggtt aatacactca agaaaaataa aagttttgat
     3721 tggcagtttg taagtagaga gaaagctgat catgagataa aaatgggtaa atattttgca
     3781 ggtatttaca tcccatctaa gtttacacat gaaattacag ggacactacg taagcagcct
     3841 caaaaagcag atgtagaatt taaggtgaat cagaagatta acgctgttgc gtctaagcta
     3901 acagatactg gttcgtcagt tgtcgttgaa aaagcgaatg aacaatttaa taaaacagta
     3961 actcgagcat tattagaaga agctaacaaa gcaggtttaa ctattgaaga aaatgtgccg
     4021 acaattaaca agataaaaaa tgcggtatat tcagcagata aagctttacc taagattaat
     4081 gactttgcga ataaaattgt atatttgaat aaccaccaag cggatttaga taaatatgcc
     4141 aatgatttta gaaaactagg aaattataaa ggtgatattt tagatgctca gaaaaaatta
     4201 aacgaagtca atggtgctat tccgcaactt aatgaaaagg ctaagttgat attagcttta
     4261 aataattata tgccgaaaat tgaaaaagcg ttaaattttg cagctgatga cgtgccagcg
     4321 cagttcccta aaattaatca aggacttaac attgcgagtc aaggtattga tcaagctaat
     4381 ggacagttaa atgatgccaa aggcttcgtc acacaagtta gaagtagagt cggtgattat
     4441 caagatgcaa ttcgacgcgc gcaagattta aatcgaagaa accagcaaca gattcctcaa
     4501 aatagcgcgg cgaacaacga aacatcaaat agtgcacctg cagctggtaa tggtgtagca
     4561 tcaacgccac caagtgcacc aagtggcgat actgcaccaa ataataatgt tacgcaaaat
     4621 accgcaccaa atagtaataa tgcgcctgta tcgactacac cacaaagtac aagcgggaaa
     4681 aaagatggtc aaagttttgt agatataaca acaacacaag tcagcacagc taacgagaac
     4741 acacaaaaca ttacagataa agatgttaaa tcaatggaag cggcattaac gggctcttta
     4801 ttatcattat caaataattt agatacccaa gcgaaagccg cacaaaaaga tagtcaggca
     4861 ttacgtaata tttcgtatgg gattttagca tcggacaagc cttctgattt tagagagtct
     4921 ttagataatg ttaagtccgg tttagaatac acaacgcaat ataatcaaca atttatcgat
     4981 acattaaaag agattgagaa gaatgaaaat gttgatttat caaaagaaat tgataaggta
     5041 aaagcagcta ataatcgaat taatgaatca ttaaggttag ttaatcaatt aagcaatgca
     5101 ttaaagaatg gtagttcagg aactgctgaa gctactaaat tactagatca actttcaaaa
     5161 ctagattcat cattatcatc atttagagat tatgttaaaa aagatcttaa cagctcttta
     5221 gtatcaatat cacaacgtat tatggatgaa ttgaacaaag ggcaaactgc attatccaat
     5281 gttcagtcta aattaaatac aattgatcaa gtcattaaca gtggacaagc tattttaaaa
     5341 aatggtaaaa cacgtattga tcgcttacaa acagtattac caagtattga acaacaatac
     5401 attagtgctg ttaaaaatgc tcaagcaaac ttctcgaaag tgaaaagtga tgtagctaaa
     5461 gctgctaact ttgtgcgcaa tgacttacca cagttagaac agcgattaac taatgcgaca
     5521 gcaagtgtga ataaaaattt accaacgtta ttaaatggtt atgatcaagc ggtaggatta
     5581 ctaaataaaa atcagccaca agcgaaaaag gctttatcag atttagctga tttttctcaa
     5641 aataaattgc ctgatgttga aaaagatttg aaaaaagcga ataaaatttt caagaaatta
     5701 gacaaagatg atgcagtcga caaattaatc gacacactta agaatgattt gaaaaagcaa
     5761 gcgggtatta ttgcaaatcc tattaataag aagactgttg atgttttccc agttaaggat
     5821 tatggttcag gtatgacacc attctatact gcactgtcag tatgggtagg tgcactcttg
     5881 atggtaagtt tattaacggt tgataataaa cataagagtc tagagtcagt cttaacgaca
     5941 agacaagtgt tcttaggtaa ggcaggattc tttataatgc ttggtatgtt gcaagcactc
     6001 attgtatcgg ttggagattt gttaatccta aaagcaggag ttgagtcacc tgtattattt
     6061 gtacttataa cgattttctg ttcgattatt ttcaactcaa tcgtatatac gtgcgtatca
     6121 ttacttggta acccaggtaa agccattgca atcgtattgc ttgtattaca aattgcaggt
     6181 ggtgggggaa cattcccaat tcaaactacg ccacaatttt tccaaaacat ttcgccatac
     6241 ttaccattta cgtatgcaat tgattcatta cgtgaaacag taggcggtat tgttccggaa
     6301 atcctaatta caaaattaat tatattaacg ttatttggta taggattctt cgttgtaggt
     6361 ttaattttaa aacctgtaac agatccattg atgaagcgcg tatctgaaaa agttgaccaa
     6421 agtaacgtta cagaataaaa attaaatcca cacattaggg ttatagctcc ttaatgtgtg
     6481 gatttttatg tttttagaca gaagagatag taatttctgt cttttatggg acggttgtta
     6541 tcattgctat tatccaggat gacttactat agcactaata ttaccgacaa agtgaatatc
     6601 ctcgtcttcc gtagttaaaa taaagctaga acctttttgg atgtcatagt gcttatcgtt
     6661 tactgttaaa gtaccagtac catcgataat tgtaactaag caataagcat gtggtttatt
     6721 gaattttaaa tctccatgaa tatcccattt atatactgca aaatattgat tatctacaaa
     6781 ttgagttaca gtgtgtgtgt cgatgtgagt tgttatagga gtagtatttg gttcatgatt
     6841 gcctaattca atcacatctt tactttgctc taagtgcaaa tcacgcaatt gaccattttg
     6901 atctcgtcta tcatagtcat aaatacggta tgtcgtatcg gaggattgtt gtgtctctaa
     6961 aattaaaata cccgaaccaa tggcatggac agtgccagca ggaacataat aaaagtcacc
     7021 gggcttaaca ggtatacgtt tgaaaagact gtcaaattca tgattatcaa tcatgtctat
     7081 taacgtctgt ttattatgtg catgtacgcc atatataatt tcagcacctg gggctgcatc
     7141 taaaatatac caacattctg ttttacctag ttcgccttcg tgttttaaag cgtagtcatc
     7201 atctgggtga acttgaacag ataatttatc attggcatct aatactttag ttagcagagg
     7261 gaaactatct cgtgaatcat tatcgaataa ttcacgatgt tgtgaccaaa gttgatctag
     7321 ggtcatatcc ttgtatggac cattgataat tgtattagga ccatttggat gtgcagaaat
     7381 tgcccagcat tcaccagttg tttcattagg gatatcatag ttaaatgctt ttaatgcatg
     7441 accgccccaa attctgtctt taaaaacggg ttgtaaaaat aatgccatag ttaaaactcc
     7501 tctatatttt cattaataag ttataaattt ctgtagtact gtttgcatta attagtgatt
     7561 ggcgtgtctc atcattcatt aacgctttag ataagcgctg aagtattttt aaatgtgtat
     7621 cctgactgtt gtttggtacg gcaattaaga atatcaattg aggtagacta ccatctagac
     7681 tgtcccattt aacaccatga ttatttttca taacagctac aatcggttgt tttacaacat
     7741 cagactttgc atgtggaatg gccacgttca tgccaatagc tgtcgtagac cccatttcac
     7801 gttctagtat tgcatttttt aaatgcgatg tgtgctctac ataacggcaa attttaagtt
     7861 tatgaatcaa catatcaatt gcttcgtttc gagacatgtc gtgatcagta attatcatag
     7921 tttgttgatc aaaaacatga gaaggtttat tgagatgtga atgtttcgcg gtgttatcta
     7981 cattgtcaac ctctgtatca tgttgtgtaa tatctgtatc atgaagttgc gtgtgttgcg
     8041 ctggtgcatc tactgctata actggtgtat tgcgttttaa taatagtaca gtagtcattg
     8101 tgacaagact acctactatc actgcaaaga taaaccataa tacatgatca ataccaccta
     8161 atacagccac gattggacct ccatgtgcga ctctatcgcc gacaccacca atggctgcaa
     8221 tgactgatgc aatcattgca ccaatgatgt ttgcaggtat aatgcgcaat ggatcttggg
     8281 ctgcgaaagg aatagcacct tcagtaatac caaatagtcc catagtgaag gaagccttac
     8341 ccatttctct ttcggaatga ttgaatttat acttttgaac aaacgttgct aaacctaaac
     8401 cgattggtgg tgtacataca gcaactgcga ccatacccat aacggcgtaa ttaccttcag
     8461 caataagtgc tgagccaaat aaaaatgcta ccttgtttac tggaccgccc atatcgaagg
     8521 caatcatcgc acctataatc atcgcaagta taataatatt agcaccttgc atacttttta
     8581 accaggttgt taatgcctca aaaatattag aaattggtgc accgattaaa aatataaata
     8641 tcaatcctac aacgaccgat gaaataatgg gaataataat gataggcata attggtgcca
     8701 ttgcttttgg aactttaata tctttaatcc actttgcgat ataacctgct aagaaaccag
     8761 caacaatacc acctaaaaat cctgcgcctg catcactgcc ataaaaacta ccgtcagcag
     8821 cgatagcgcc gccaatcata ccaggaacaa gaccgggctt gtcagcgata ctaacagcga
     8881 tatatccagc tagtattgga accataaatt taaaggctaa actaccaatg ttttcaatgg
     8941 atttccaaaa tgaatcatct gggatgacta atccttttga tgtcgtttca ccgcctagag
     9001 tcagcgcgat ggcgataagg agtccaccaa ctacgataaa aggaaccata aacgatacac
     9061 cgttcattaa atgttgatac accatttgaa taccactttt agacttaccg cgatctttcg
     9121 aatgataatt tgtttcagat tgataaatag gcgcatcttg attaatgata cgttgaatta
     9181 gacctctcgg attatgaatc ccttcgcgaa cattttcatt aatcaaccgt ttaccaacaa
     9241 atcgggacag atcaacttgt ttatcagctg caattatgac accgtcagct tcttcgatgt
     9301 cttgcgtagt taaaacattt tcagcaccaa caccgccctg tgtctctact ttaatatcca
     9361 cacccatttc ttttgctacc tgctcaagct tttcttgagc catatatgta tgtgcaatgc
     9421 catttgggca tgaggtaata gctacaattt tcataaaatc atctcctttt ctatattgta
     9481 agcgtattct cgatactaaa aaaaagaata attaccgtta ctagtggcaa ttattcttgt
     9541 aagtattcaa ataactgttg ctttaaacta tgatcatcta aactacataa atggttcact
     9601 gaatcatcat ccaagttagc aattaattgc atcatttgtt ttgtaaaagc tttgtcttta
     9661 tgcgaaatcg ctaagaaaaa gacaagtttg acatcgtgtt gtcgccaagg aaaaacatct
     9721 tttgtgcgaa aaataagcac atgtgattgt aaaacttttt caggatctcc atgaggaatc
     9781 gccataaaat tacctatgta tgtagaagat gatttctcac gctctaaagc tgattcgata
     9841 tatccttcta caatcgcatg atgtgcttgt aatatttttt gagcttcttc aaaaatttgc
     9901 acagtatgcc gtgatttttg ttcagtattt acgacaagga aattgacagt gtccatatga
     9961 tgatgtgctt gaaccggatt ttgcttttgc ttcacaacgt gtctgatttt gtgacgatca
    10021 tcttcagaaa ataatggtgc aaccttgata gtcgtcaggt gcttaggaag tatgtttagc
    10081 gtttgtttag gaatatcatg ggtcgttatt aataaatcta cattgtcaaa gtgatagtgt
    10141 gttatatttt ctagtttaat cgtatttatc actgacaact cttcggataa gttatttatt
    10201 ttagtttcta aaaaattcga cacacctaga ccataataac aagcaatgac tacatttaat
    10261 tgtgttttgg tacgacgctc gatggcagct tgaaaatgaa ttgttaaaaa tgcaatttca
    10321 tcttcgctca tctctatatc agtatcaatt gctaatttat caatcgcttc aaaaagtgtg
    10381 ttaaacacaa agggatagag ttttttaatc tctataacta aaggattgtt taaataaatg
    10441 ttttgagtga tacgtaaata tgctttacta aaatgattat ataaattttg ttgtaaaatc
    10501 gaatcttcat tgaaaggtac atgaatacgt tgctgcatca attcgattaa gcgatcaata
    10561 taactttgta taaatatacg ttctatgcca atatcgagtt tattaaaatg ataagcaata
    10621 aagaatgaaa acatattgat tactttttcg ttcaagtcat aacctaatct ttcgttgatt
    10681 tgcttaatgc aagattgaga tatcaatttt gaatcgtggt ttaacgtgtc tagctcactt
    10741 aaagattgct tacgtttaat gattaaaatg agatgaatta aaagctgttg tatttgaata
    10801 tcagttgttt caatactatg ttgttgaagt gtctcttgta taatatgcga aatcatcctt
    10861 tggtgtgaat caggtaattc atttaaaatt aggtcttcaa catgtacatg ccctgatgat
    10921 aattgattta aatggatgat ggcattagtg atatcattat ctgttccatc gaccacaata
    10981 ccttgttttt tcttgattgt taaagcgata ttaaattttt gacaccattg ttgaattcga
    11041 acgaaatagt cgttgagcac agttttagat aatagataat cttgctctag ttgttgcaat
    11101 gtctgcgatt tattgtgcat tagcagttga tacgccatag ataataatag tttttcttta
    11161 tatgattgaa tatgcgtgta acgttcagtt atcgtttcta aggtatactg agatgtattt
    11221 aattgatatc ctttagattt aatacttacg atgatatcat ccataaaatt gctattgata
    11281 acatggatat cattgcgaac tgtgcggttt gaaacattga catgtttagc aatttcatta
    11341 gaactaatgt gctttgatgg atttttaata aaaaactgga gtagttttaa gtgtctatca
    11401 agcatttaac atgtacctcc tttctaaatt tttcgtgtaa gcgtttttaa gggtgtatta
    11461 atattattaa acatgagagc ttatacatac gtcaatgaca ttaaagcgaa cttttatatg
    11521 attttaacag agtgcgaatt atgcaaataa agaacagcag taagatattt caaatagaaa
    11581 aatatctcac tgctgttttt ctgaatttat gcatctggta cttgtggacg tatcaggcaa
    11641 atgattaatt tttaggtgat tgtgcttgag gtgtttgttt agaaggcgta ttggtattat
    11701 ttgatttatt agcaggtggt gtgttctgtt gttgattatt ttggttgttt gttgacgatt
    11761 gactgccacc accgtgagta ttatttttat ttgaatttga gtcttttggc tgagctggtt
    11821 gcgtattcga tggtactgtt gaatggttgt taggaacatt tgacggtgaa gatggttgat
    11881 tgtcattagt aacgttattg ccattatttt gatttttatc tgttttgtct gcactatcat
    11941 cttgttgatc attttcttcg gtttctgtct ttttatgcgt agatttattt tcttgttctt
    12001 ggttattact tgatgatgta gaatgattta ttgcagcata aattgcgaac ccaagtgctc
    12061 cgattaatac gataattacg atggtaataa gaagtttttt caaatataac gcctccatgt
    12121 taatgaaaag tagttgatac gtgaagctaa ataatttatt tcaatattgc cataatcatt
    12181 ttaaaaaata aatattagaa aagtaggcga tattaatatt ttcattgttt tttgtgtact
    12241 tcaattagta taacaaaact gttatgtatt tataaatata atcattagta tttttatggc
    12301 tgaaaaagtt ataataaaag tgtaaggaat aaaatatttg tatggaaaag agagataatt
    12361 atgaataaaa ccagtaaagt ttgtgtagca gcaacattag cattgggcac actgattgga
    12421 gtaacagttg ttgaaaattc ggcgccaaca agtaaacagg cacaggc
//
LOCUS       DAKTIO010000075        12374 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.075, whole genome shotgun sequence.
ACCESSION   DAKTIO010000075 DAKTIO010000000
VERSION     DAKTIO010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12374
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.075"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            223..1206
                     /locus_tag="O0293_001517"
     CDS             223..1206
                     /locus_tag="O0293_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCY0280405.1"
                     /translation="MKKWQFVGTTALGATLLLGACGGGNGGSGNSDLKGEAKGDGSST
                     VAPIVEKLNEKWAQDHSDAKISAGQAGTGAGFQKFIAGDIDFADASRPIKDEEKQKLQ
                     DKNIKYKEFKIAQDGVTVAVNKENDFVDELDKQQLKAIYSGKAKTWKDVNSKWPDKKI
                     NAVSPNSSHGTYDFFENEVMNKEDIKAEKNADTNAIVSSVTKNKEGIGYFGYNFYVQN
                     KDKLKEVKIKDENGKATEPTKKTIQDNSYALSRPLFIYVNEKALKDNKVMSEFIKFVL
                     EDKGKAAEEAGYVAAPEKTYKSQLDDLKAFIDKNQKSDDKKSDDKKSEDKK"
     gene            1397..2323
                     /gene="pstC"
                     /locus_tag="O0293_001518"
     CDS             1397..2323
                     /gene="pstC"
                     /locus_tag="O0293_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HCY0280406.1"
                     /translation="MTSSTNVKALIEKNNNKKGKHNDKIIPVILAAISAISILTTLGI
                     LITLLLETITFFTRIPITEFLFSTTWNPTGSDPKFGIWALIIGTLKITVIATIFAVPV
                     GLGAAIYLSEYASDRARRIIKPILEILAGIPTIVFGFFALTFVTPVLRSFIPGLGEFN
                     AISPGLVVGIMIVPLITSLSEDAMASVPNKIREGAYGLGATKLEVATKVVLPAATSGI
                     VASIVLAISRAIGETMIVSLAAGSSPTASLSLTSSIQTMTGYIVEIATGDATFGSNIY
                     YSIYAVGFTLFIFTLIMNLLSQWISKRFREEY"
     gene            2325..3242
                     /gene="pstA"
                     /locus_tag="O0293_001519"
     CDS             2325..3242
                     /gene="pstA"
                     /locus_tag="O0293_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCY0280407.1"
                     /translation="METTDNNRQSLVDQQLVQKHLSSRTVKNKVFKLIFLACTLLGLV
                     VLIALLTQTLIKGVSHLNLQFFTNFSSSTPSMAGVKGALIGSLWLMLSIIPLSIILGI
                     GTAIYLEEYAKNNKFTQFVKISISNLAGVPSVVFGLLGYTLFVGGAGIEALKMGNSIL
                     AAALTMTLLILPIIIVSSQEAIRAVPNSVREASYGLGANKWQTIRRVVLPAALPGILT
                     GFILSLSRALGETAPLVLIGIPTILLATPRSILDQFSALPIQIFTWAKMPQEEFQNVA
                     SAGIIVLLVILILMNGVAIILRNKFSKKF"
     gene            3289..4140
                     /locus_tag="O0293_001520"
     CDS             3289..4140
                     /locus_tag="O0293_001520"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCY0280408.1"
                     /translation="MAQTLAQTKQISQSHTFDVSQSHHKTPDDTNSHSVIYSTQNLDL
                     WYGENHALQNINLDIYENQITAIIGPSGCGKSTYIKTLNRMVELVPSVKTAGKILYRD
                     QDIFDQKYSKEQLRTNVGMVFQQPNPFPKSIYDNITYGPKIHGIKNKKVLDEIVEKSL
                     RGAAIWDELKDRLHTNAYSLSGGQQQRVCIARCLAIEPEVILMDEPTSALDPISTLRV
                     EELVQELKEKYTIIMVTHNMQQAARVSDKTAFFLNGYVNEYDDTDKIFSNPSNKKTED
                     YISGRFG"
     gene            4147..4788
                     /gene="phoU"
                     /locus_tag="O0293_001521"
     CDS             4147..4788
                     /gene="phoU"
                     /locus_tag="O0293_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCY0280409.1"
                     /translation="MAIIRQRYQEQLDDLIKELRRLGANVYVSIENGIKSLSIDDRGF
                     ARQTVKNDKHINQLNYDINERVIMLITKQQPIASDLRMMISSLKIASDLERIGDNASS
                     IANIRLRTKITDDYVLTRLKTMGKLAMLMLKDLDQAFKKKDTVLIREIIERDEDIDDL
                     YSHIINATYLIDNDPFVAAQAHLAARHLERIGDHIINIAESVYFYLTGTHYEQ"
     gene            complement(4928..6742)
                     /gene="pepF"
                     /locus_tag="O0293_001522"
                     /pseudo
     CDS             complement(4928..6742)
                     /gene="pepF"
                     /locus_tag="O0293_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
     gene            6947..7291
                     /locus_tag="O0293_001523"
     CDS             6947..7291
                     /locus_tag="O0293_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280410.1"
                     /translation="MTLFDMPNYLWITTLIMILLTIFCCLVLNKWFVSAVITFVILGV
                     LAFFIPNFQDIKYQPLLGYAAFLAIMSLLISFLLWYFTRNWRKERKARKLEKQIEKYG
                     YEGAELRRKDKK"
     gene            7239..7424
                     /locus_tag="O0293_001524"
     CDS             7239..7424
                     /locus_tag="O0293_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280411.1"
                     /translation="MKNTAMRALNFAVKIKNNIRQLYAQNHRLLSYTAVTIISKEMEY
                     SAIVVFVLTFLYFTWLT"
     gene            7595..8581
                     /locus_tag="O0293_001525"
     CDS             7595..8581
                     /locus_tag="O0293_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0280412.1"
                     /translation="MFIIKSMLYRLMQMIVVLFVISTLTFILMKLSPGNPVDKILHLD
                     VAQVSTEQINATKDKLGLNDSLLVQWWHWMNHLLHFNLGKSFESKEPVTQILFNYAPI
                     TLLISFSTLVVSLCISIPLGIIAAKRFHKWTDKVIRVISTLSISLPAFFIGIILLFIV
                     TNLMNIDSVILSQFILPVITLSLGMCAYIIRLVRSNLLMLLQSNIVQASRLRGMNERY
                     ILIHDLLKPTILPIIPLLGISLGSLIGGTVVIENLFDIPGIGYLLMDSIKSRDYPVIQ
                     GCVLFIGFFVVIINTIADLLTLLLDPKQRLQLGNPKIKTNTPLISESSDRHA"
     gene            8574..9404
                     /locus_tag="O0293_001526"
     CDS             8574..9404
                     /locus_tag="O0293_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0280413.1"
                     /translation="MHKIFSKNNLIFFVFVAFIFVVIVLQFFVSSENATKVNLSQTFE
                     PISWLHLLGTDDYGRDLFTRIIIGARSTLFVTVLTLIAIVVIGVTLGLFAGYKKGWIE
                     RLVLRFIDVGLSIPEFIIMIALASFFQPSLWNLVISITLIKWMNYTRLTRSIVNSEMN
                     KPYIKMAQLFHVPTRTILIRHLTPKIIPAIIVLMVVDFGKIILYISSLSFIGLGAQPP
                     TPEWGAMLQQGRDFISSHPIMLIAPASVIAITILIFNLTGDALRDRLLKQRGEYDESH
                     "
     gene            9391..10164
                     /locus_tag="O0293_001527"
     CDS             9391..10164
                     /locus_tag="O0293_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0280414.1"
                     /translation="MSLIDIQNLTIKNTSEKSLIKGIDLKIFSQQINALIGESGAGKS
                     LIAKALLEYLPFDLSCTYDSYQFDGENVSRLSQYYGHTIGYISQNYAESFNDHTKLGK
                     QLTAIYRKHYKGSKEEALSKVDKALSWVNLQSKDILNKYSFQLSGGQLERVYIASVLM
                     LEPKLIIADEPVASLDALNGNQVMDLLQHIVLEHGQTLFIITHNLSHVLKYCQYIYVL
                     KEGQIIERGNINHFKYEHLHPYTERLIKYRTQLKRDYYD"
     gene            10157..10858
                     /locus_tag="O0293_001528"
     CDS             10157..10858
                     /locus_tag="O0293_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0280415.1"
                     /translation="MIELKHVTFGYNKKQMVLQDINITIPDGENVGILGESGCGKSTL
                     ASLVLGLFKPVKGEIYLSDNAVLPIFQHPLTSFNPDWTIETSLKEALYYYRGLTDNTA
                     QDQLLLQHLSTFELNAQLLTKLPSEVSGGQLQRFNVMRSLLAQPRVLICDEITSNLDV
                     IAEQNVINILKAQTITNLNHFIVISHDLSVLQRLVNRIIVLKDGMIVDDFAIEELFNV
                     DRHPYTKELVQAFSY"
     gene            10982..11455
                     /locus_tag="O0293_001529"
     CDS             10982..11455
                     /locus_tag="O0293_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCY0280416.1"
                     /translation="MGLLDIASIRSIERGFNYYQSECVINLKSFSETQHEAEVKGSGN
                     KVYRCYIDMEHPRKSICNCPHADGRRVICKHMIALLFTASPEAANKHIMMLNEVEEDY
                     QLRRNMWIDSLKEMINDMSEEELRDAYLNMLIEHGEMAELFGLDEEEEMFEDEFY"
     gene            complement(11503..12270)
                     /locus_tag="O0293_001530"
     CDS             complement(11503..12270)
                     /locus_tag="O0293_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCY0280417.1"
                     /translation="MRFVFDIDGTLCFDGRLIDQTIIDTLLQLQHDGHELIFASARPI
                     RDLLPVLPSVFHQHTLIGANGAMISQQSKISVIKPIHTDTYHHIFKIIQKYELDYIID
                     DDWNYAAQLDAENAIFERLDPHKLASCIDVANIDTPIKIILLNIDPAQITTILDELDK
                     YHQELEMIHHSNEYNIDITAQNINKYTALQYIFDADVKYIAFGNDHNDIVMLQHASSG
                     YIIGPSEAYTHAILKLDKIKHINNNAQAICKVLKSYK"
BASE COUNT         4261 a         1827 c         2118 g         4168 t
ORIGIN      
        1 tttttgtatt catatttaaa tttcgatgat ttttcaggaa gcattttaat tttactaatg
       61 aagcaatatt ttttagatta acaaaaatta atatttacat tttcttaaca attttttatg
      121 taacatttac agtttctaaa aatgaggtta ataattcaag gttaagataa agatgtaatc
      181 aatacaaata ctatttgttg ttcatacagg gaggatattt caatgaaaaa atggcaattt
      241 gttggtacta cagctttagg tgcaacacta ttattaggtg cttgtggtgg cggtaatggt
      301 ggcagtggta atagtgattt aaaaggggaa gctaaaggtg atggctcatc aacagtagca
      361 ccaattgtgg agaaattaaa tgaaaaatgg gctcaagatc actcggatgc taaaatctca
      421 gcaggacaag ctggtacagg tgctggtttc caaaaattca ttgcaggaga tatcgacttc
      481 gctgatgctt ctagaccaat taaagatgaa gagaagcaaa aattacaaga taagaatatc
      541 aaatacaaag aattcaaaat tgcgcaagat ggtgtaacgg ttgctgtaaa taaagaaaat
      601 gattttgtag atgaattaga caaacagcaa ttaaaagcaa tttattctgg aaaagctaaa
      661 acatggaaag atgttaatag taaatggcca gataaaaaaa taaatgctgt atcaccaaac
      721 tcaagtcatg gtacttatga cttctttgaa aatgaagtaa tgaataaaga agatattaaa
      781 gcagaaaaaa atgctgatac aaatgctatc gtttcttctg taacgaaaaa caaagaggga
      841 atcggatact ttggatataa cttctacgta caaaataaag ataaattaaa agaagttaaa
      901 atcaaagatg aaaatggtaa agcaacagag cctacgaaaa aaacaattca agataactct
      961 tatgcattaa gtagaccatt attcatttat gtaaatgaaa aagcattgaa agataataaa
     1021 gtaatgtcag aatttatcaa attcgtctta gaagataaag gtaaagcagc tgaagaagct
     1081 ggatatgtag cagcaccaga gaaaacatac aaatcacaat tagatgattt aaaagcattt
     1141 attgataaaa atcaaaaatc agacgacaag aaatctgatg ataaaaagtc tgaagacaaa
     1201 aaataataag acgcaatttc aaatgtgtct tgaaacatga ttttgatggt gaatcattat
     1261 ttagagtaca aagcttgatt tatcgagacg ctgattttga cattcagtta gtctacaagc
     1321 ttatcaactt aaaatagtgg ttcatcatta ttttacaaat ctaattattt tgggagtaat
     1381 agaaagaggt ttgattatga cttcatctac taatgttaaa gctttaatcg aaaaaaataa
     1441 taataaaaaa ggaaagcata atgacaaaat tataccagtt attttagccg caatttcagc
     1501 gatttccatt ttaacaacac taggtatatt aatcacattg cttttagaaa ccatcacttt
     1561 tttcaccaga attccaataa ctgaatttct attttctact acttggaatc ctaccggttc
     1621 agaccctaag tttggtatct gggcattgat aatagggact ttaaaaatca cagttattgc
     1681 gactatattt gcagttccag tcgggcttgg tgcagcaatt tatttaagtg aatatgcaag
     1741 tgaccgtgca cgacgcataa ttaaaccgat attagaaatt ttagcaggaa taccaacaat
     1801 tgtgtttggt ttctttgcat taacctttgt tacaccagta ttaagatctt tcataccagg
     1861 tcttggagag tttaatgcta taagtcccgg cttagttgtc ggtattatga ttgtccctct
     1921 catcacaagt ttgagtgagg atgcaatggc atctgtacca aataaaattc gagaaggtgc
     1981 ctatggactt ggagcaacta aattagaagt agcaactaaa gtcgtacttc ccgcagcaac
     2041 atcaggtatt gtagcttcaa tcgttctcgc gatttcaaga gcaattggag aaacgatgat
     2101 tgtatcatta gcggcaggta gttcgccaac agcttcatta agtttaacaa gttcgattca
     2161 aacaatgact ggatatattg ttgagatagc gacaggtgat gcaacatttg gatcaaatat
     2221 ttattacagt atttatgctg tagggttcac actatttatc tttaccttaa tcatgaattt
     2281 actttctcag tggatttcta agcgttttag ggaggagtat taatatggaa acgacagata
     2341 ataatagaca atcactcgtc gatcaacaac ttgtccaaaa acatttatca tccagaacgg
     2401 ttaaaaataa agtgttcaaa ctcatatttt tagcatgtac attattagga cttgtcgtac
     2461 ttattgcgtt gttaactcaa acattgatta aaggggtaag tcatttaaat ttacagtttt
     2521 tcactaattt ttcttcttca acaccatcta tggctggcgt taaaggcgcg ttaatcggtt
     2581 cactttggtt aatgttaagt atcattccat tatcaatcat cctaggaata ggtacagcta
     2641 tatacttaga agaatatgcg aaaaacaaca aatttactca gtttgttaaa atcagtattt
     2701 ccaatttagc tggtgtacca tcagttgtat ttgggttatt aggttatact ttgttcgttg
     2761 gtggtgcagg gattgaagcc ttgaaaatgg gtaacagtat attggcagca gcgctaacaa
     2821 tgaccttact gatattacca attattattg tttcaagtca ggaagcaatt agagctgtac
     2881 ctaactcagt acgcgaagct tcttacggct taggtgctaa taaatggcaa acgataagac
     2941 gtgttgtctt accagcagcg ttacctggta ttttaactgg attcattttg tctctttcaa
     3001 gagcactggg agaaacagcg ccacttgtgc taatcggtat accgactata ttattggcaa
     3061 cacctagaag tatattggat caattttcag cattacctat ccaaatattt acttgggcga
     3121 aaatgcctca agaagaattc cagaatgttg catcggcagg cattatcgtt ttactagtta
     3181 tcttaatctt aatgaatggc gttgcgatta ttttacgtaa caaatttagt aaaaaattct
     3241 aatttaaaca atcaatctca tttatctatt aaaaagggag ttttaaatat ggcgcaaaca
     3301 cttgcacaaa ctaaacaaat atctcaaagt catacgtttg atgtctcaca aagtcatcat
     3361 aaaacaccag atgatacaaa ctcacattct gttatatatt caacacaaaa tttagactta
     3421 tggtatggcg aaaatcatgc attacaaaat attaatttag atatttatga aaaccaaatt
     3481 actgccatta taggtccatc tggttgtggc aaatcaacat atattaaaac tttgaatcga
     3541 atggttgagt tagtaccttc tgtgaaaaca gctggtaaaa tattatatcg agatcaagac
     3601 atttttgatc aaaaatattc taaagaacaa ttacgtacaa atgtgggcat ggtctttcaa
     3661 caacctaatc catttccaaa atcaatatac gataatatta cttacggtcc aaagattcac
     3721 ggtattaaaa ataaaaaagt tcttgatgaa atcgttgaga aatcattacg tggcgctgca
     3781 atttgggatg aattaaagga taggttgcac acaaatgcat atagtttatc cggtgggcaa
     3841 caacaacgtg tttgtatcgc gcgttgttta gcaattgaac ctgaagtcat tttaatggat
     3901 gaaccgacat cagcattaga tccaatctca acattaagag tagaagagtt ggttcaagaa
     3961 ctaaaagaaa agtatacaat tattatggtt acacataata tgcaacaagc agctcgtgta
     4021 tcagataaaa ctgcattttt cttaaatggt tatgtcaatg aatatgatga tactgataaa
     4081 attttctcta acccatcaaa caagaaaaca gaagattata tttcaggaag gtttggttga
     4141 tatataatgg caataattag acaacgatat caggagcaac ttgatgattt aataaaagaa
     4201 ttacgtcggt taggtgcaaa tgtctatgtg agtattgaaa atggtataaa atcattaagt
     4261 attgacgata gaggctttgc acgacaaaca gttaaaaacg ataaacatat caatcaatta
     4321 aattatgata ttaatgagcg agttatcatg ttaattacaa agcaacagcc cattgcgagt
     4381 gatttgcgta tgatgatttc ttcattaaaa atcgcctccg atttagaaag aataggagat
     4441 aatgcctcga gtattgccaa tattcgattg cgtacaaaga ttacagatga ttatgtgtta
     4501 acccgtttaa agacaatggg taaattagct atgttaatgt taaaggactt agatcaagca
     4561 tttaaaaaga aagataccgt attaataaga gaaataattg agcgtgatga agatatcgat
     4621 gacttatata gtcatattat taacgcaacg tatcttattg ataacgatcc atttgtcgct
     4681 gcacaagctc atttagcagc aagacattta gaacgtattg gtgatcatat tattaacatc
     4741 gctgaaagtg tttattttta tttaacaggt acacattacg aacaataact taaagttatt
     4801 actataaaat cccttacgat aaatatatat ttctattatt cataaaccct caaaaaaacc
     4861 aagattctca caattagtaa tgtgaaaatc ttggtttata ttgttctact ataaattgtc
     4921 tcgcatctta gttatttgct tgctcaattt catctgttaa tttttcaact tcatcgacta
     4981 aatcagaaat atattgaatt gtagatttaa gtggctgttc tgtagtaatg tctacacctg
     5041 caatgtttgc aagttcgaca ggtgatacac taccaccttt tttcaatgtt tctaaccaag
     5101 catcaacagc tggttggcct tcatttttaa tcttttgaga aacgacagtt ccgattgtta
     5161 agccagcaga atacgtatac gaatataatc ccatatagta atgaggttga cgcatccatg
     5221 ttaattcagc accctcagtc atgtctactg catctccaaa aaattgttta taaacattta
     5281 gcattatttc atttaatgtc ggcgcattta aagattcacc ttgatctact ttgtgataca
     5341 cttcacgttg ataagcagct tctaataaat gggtaaccat attatgataa tatgttctag
     5401 ataaaattga gccaataacc caacgcttaa atcttggatt atcacttgtg ttaaataaat
     5461 aattggccat caacatttca ttcattgtag aaggggcttc aacaaagtac attgatgctt
     5521 ctgattcaag atatggttga tgtttttgag ctaatgtaaa atgacctgca tgacctaatt
     5581 catgtgctaa gacaaatgct tcagccattt taccagtcca agaaataaac acatatgaat
     5641 gtgtaaagta tggacttgca caaaatgcgc ctgtatcttt acctttattt tgtgcaaaat
     5701 caatccatcg ctgatcgtat gcttcacgta acatgtttgt atagtcatca cctaaaacac
     5761 ttaacgcacc gaaaatataa ttttttgagt cttcaattga aatctctggt tcataatcag
     5821 ggtctacaga aatcttcaag tcttcaaaac gcatgttatc taatccgtga atacgttgta
     5881 aaagtttagc atatttctgc ataactggtg ccaagtcacg cataatcata tcgatttgac
     5941 ggtcaaacat atcacgcgtt acttcttgac tatgtaataa ataatcgatg actgattcaa
     6001 atccacgtaa atcagcttca attttttctt gttgtacttg catattatat gtagccgcgg
     6061 tagtatgctg atattttcga atcccatcgc taaacgattt gaaactttta cgtctaaact
     6121 caggatcttt attatcttca taatcatttt caaacgtagc atagtcgaca gggtacgttg
     6181 taccatcatg ttcaaatgaa tcgaatgtaa tatctagcat ttttgtcgtg ccgtataaat
     6241 cgtaaggact gtttagcgta ggtgataaag ttgctaatac ttgttctacc gacgcagata
     6301 attggaatgg cttttgtttt attaactgtt taatatagtg ttgatatgga catgattctt
     6361 ctaattgttg aagtatttct tctggtaatt caagtatttc tgactctaca aatgataatt
     6421 ggctaacaat tttaccgtat gtagtagata atttagcgct caatacttgt gcctcgatat
     6481 tactagtatc tacacttaaa cgtagttctg cataattact taagcgatct aaggcaatta
     6541 aaatattttc taattcagct aaagcagtat taatttgttc gattgaattt aatgttgttg
     6601 catatgtatg atgaaattga tttgcttgtt gtactagagc gtcaatactt tcataatatt
     6661 gttgatcatc tttaaataag tctactaaat cctatgtttc tgaaacagga acatcttctc
     6721 ttaaaggtaa accttgagac atataatcac tcctttggta taatacattc attatacata
     6781 attttgtctc ctaaacgata atgttacgat ttccatgtcg caaggaacaa tgatttttct
     6841 attagtagat agaatattgc ttgaaactga ctgctattgt ttaagttttt aaaaatttat
     6901 aataaaatgt atacaaggaa agtaatgaaa taaaaggaga atgaatatga cattattcga
     6961 tatgcctaat tatttatgga tcacaacatt aataatgatt ttattaacaa tattctgttg
     7021 cttagtttta aataaatggt ttgtatctgc agtaattaca tttgttatat taggtgtgct
     7081 tgcatttttt attccaaatt ttcaagatat aaaatatcaa ccattattag gatacgctgc
     7141 atttttagcg attatgagtt tattaattag ttttctatta tggtatttta ctagaaattg
     7201 gcgtaaagaa agaaaagcgc gtaaattaga aaagcaaatt gaaaaatacg gctatgaggg
     7261 cgctgaactt cgccgtaaag ataaaaaata atattcgaca actatatgca caaaatcatc
     7321 gacttttaag ttacactgca gtaacaatta tttcgaaaga gatggaatac agtgctatag
     7381 ttgtttttgt tttgactttt ttatatttta catggttgac ttgattcaaa atattatatt
     7441 ttataatgat tttgttaaaa aattaaatat acttcaattt ctttatggat aatactataa
     7501 tgaacgttag ttcttaatat gtaagttgca tatacatgcg ccattctcat gtaaaaagaa
     7561 agtgttagta acttacacaa ggagggggac tattatgttc ataataaagt ctatgttgta
     7621 tagactgatg caaatgatag ttgttttatt tgttatttct acacttacat tcattttaat
     7681 gaaattatca ccaggcaatc ctgttgataa aatattacat cttgatgtag cacaagtgtc
     7741 gacggaacaa ataaatgcaa ccaaagataa attgggatta aatgattctt tattggtaca
     7801 gtggtggcat tggatgaatc atttattaca ttttaattta ggaaaaagtt ttgaatcaaa
     7861 agaaccggta acacagatat tattcaacta tgcacccata acattactca tatcattttc
     7921 aacattagta gtgtcgttat gcatatctat accgctcggc attatagcag ccaaacgatt
     7981 tcataagtgg acggataaag tgattagagt gatctcaaca ctatcgatta gtttaccagc
     8041 gttctttatc ggtattattt tattatttat tgtcacaaat ttaatgaata tagatagcgt
     8101 tatactaagt cagtttatat tacctgtaat cacgctatct ttaggtatgt gtgcatacat
     8161 cattcgttta gtgcgttcta atttattgat gttattgcaa agtaatatcg tacaagcatc
     8221 aagattacgc ggtatgaatg aacgttatat tttaattcat gatttactaa aaccaacaat
     8281 tttgccgatt atcccattac tagggatttc acttggcagt ctaataggtg gtactgtagt
     8341 gattgaaaat ttatttgata tacctggtat tggttatcta ttaatggata gtattaaatc
     8401 tcgagattat cctgttattc aaggatgcgt gttatttatt ggcttcttcg ttgttattat
     8461 caatacgatt gctgatttat taacgttatt acttgatccg aagcagcgtt tacaattagg
     8521 aaatccaaaa atcaaaacca atacaccatt gatatcagaa agtagtgacc gtcatgcata
     8581 aaatattttc aaagaataac ctgatatttt ttgtattcgt tgcatttatt tttgtggtaa
     8641 ttgtactgca attctttgtc agtagtgaaa atgcaaccaa agtcaattta tcacaaactt
     8701 ttgaacctat tagttggttg catttattag gaactgatga ttatgggaga gatttattta
     8761 cccgaattat tatcggtgca cgttcaacat tgtttgttac tgttttaaca ttaatagcta
     8821 tcgttgtcat aggtgttaca ctaggtctat ttgccggata caaaaaaggg tggattgaac
     8881 gattagtgtt aaggtttatt gatgttggtc taagtattcc agaatttatc atcatgattg
     8941 ctttagcaag tttttttcaa ccatctttat ggaatttagt tatctcaatt acattaataa
     9001 aatggatgaa ttacacaagg ttgactagaa gtatagttaa tagcgaaatg aataagcctt
     9061 atataaaaat ggcacaatta tttcatgtac caacaagaac aatattaata cgtcatttaa
     9121 cacctaaaat tataccggct attatcgttt tgatggtcgt tgatttcggt aaaatcattc
     9181 tatatataag ttcactatca tttattgggt taggtgcaca accgccaaca ccagagtggg
     9241 gcgctatgtt gcaacaaggt cgtgatttta tttcgtctca tccaattatg ttgattgcac
     9301 ctgcttcagt cattgctata actattttaa tttttaattt aaccggtgat gcactaagag
     9361 atagattgct gaaacaacgg ggtgaatatg atgagtctca ttgatataca aaatttaaca
     9421 ataaagaata ctagtgagaa atctcttatt aaagggattg atttgaaaat ttttagtcaa
     9481 cagattaatg ccttgattgg agagagcggc gctggaaaaa gtttgattgc taaagcttta
     9541 cttgaatatt taccatttga tttaagctgc acgtatgatt cgtaccaatt tgatggggaa
     9601 aatgttagta gattgagtca atattatggt catacaattg gctatatttc tcaaaattat
     9661 gcagaaagtt ttaacgacca tactaaatta ggtaaacagt taactgcgat ttatcgtaag
     9721 cattataaag gtagtaaaga agaggctttg tccaaagttg ataaggcttt gtcgtgggtt
     9781 aatttacaaa gcaaagatat attaaataaa tatagtttcc aactttctgg gggccaactt
     9841 gaacgcgtat acatagcaag cgttctcatg ttggagccta aattaatcat tgcagacgaa
     9901 ccagttgcat cattggatgc tttgaacggt aatcaagtga tggatttatt acagcatatt
     9961 gtattagaac atggtcaaac attatttatt atcacacata acttaagtca tgtattgaaa
    10021 tattgtcagt acatttatgt tttaaaagaa ggtcaaatca ttgaacgagg taatattaat
    10081 catttcaagt atgagcattt gcatccgtat actgaacgtc taattaaata tagaacacaa
    10141 ttaaagaggg attactatga ttgagttaaa acatgtgact tttggttata ataaaaagca
    10201 gatggtgcta caagatatca atattactat acctgatgga gaaaatgttg gtattttagg
    10261 cgaaagtggc tgtggtaaaa gtacgctcgc ttcattggtt cttggcttgt ttaaacctgt
    10321 taaaggagag atttacttaa gtgacaatgc tgtgttaccg attttccaac accctttaac
    10381 tagctttaac cctgattgga cgattgagac ctcattaaaa gaagcgttat attattacag
    10441 aggtctaact gataatactg ctcaggatca attattatta caacatttat ctacttttga
    10501 gttaaacgcg caattattga ctaaattacc aagcgaagtg agtggcggac aattacaaag
    10561 atttaatgtc atgcgttcgt tattagcaca gcctcgcgtt ttaatatgtg atgagataac
    10621 ttcaaattta gatgttatag ctgaacaaaa tgtaatcaat atattaaaag cgcaaacgat
    10681 tacgaactta aatcatttta tcgttatttc tcatgattta tccgtgttac aacgcttagt
    10741 taatagaatt atcgttctta aggatggcat gatagtcgat gattttgcaa tagaggaatt
    10801 atttaatgtt gatagacacc cttatacaaa agaattagtg caagcatttt catattagtt
    10861 atttaagaat gcgataattc tagacttgtt ataaaatata gataaatcaa gtattttaat
    10921 ctagacactt atctatttta ttttctttat ttaaaaataa taataaaaag gagtatcatt
    10981 aatgggatta cttgatattg caagtattcg ttctatagaa agaggcttta attattatca
    11041 aagtgaatgc gtcattaact taaaatcatt ttcagaaacg cagcatgagg ctgaagtaaa
    11101 gggcagtggc aacaaagtat atcgttgtta tattgatatg gaacatccta gaaaatccat
    11161 atgtaattgt cctcatgctg atggaagacg agtgatatgt aaacatatga ttgcattact
    11221 ctttacagct agtccagaag cagcaaataa acatataatg atgttaaacg aagttgaaga
    11281 agactatcaa ttacgcagaa atatgtggat tgatagtctt aaagaaatga ttaatgatat
    11341 gagtgaagaa gaactccgcg atgcatattt aaacatgtta attgaacatg gagaaatggc
    11401 agaattattt ggattagatg aagaggaaga aatgttcgag gacgaatttt attaaaatag
    11461 cccctatcga ttgataatga ttatcatttg ataggggtgt ttttatttat atgattttaa
    11521 gactttgcaa atagcttgtg cattattatt gatgtgtttg attttatcaa gtttcaatat
    11581 tgcgtgtgtg tatgcttctg atggtcctat aatatagcca ctactagcat gttgtaacat
    11641 gacaatatca ttgtggtcat taccaaatgc tatatattta acatctgcat caaatatata
    11701 ttgtaatgca gtatatttgt taatattttg cgctgttata tcaatgttat actcatttga
    11761 atggtgaatc atttccaatt cttgatggta tttatctagc tcgtctaata tagttgtaat
    11821 ttgtgccggg tctatattta ataaaataat cttgattggt gtgtcgatat ttgcaacatc
    11881 aatacaactg gccagcttat gtggatctaa acgctcaaaa atcgcgttct cagcgtcaag
    11941 ttgtgcagca taattccaat catcatcaat aatataatct aactcatact tttgaattat
    12001 tttgaagata tgatgatatg tatcagtatg aattggtttg ataacagaaa tctttgattg
    12061 ctgtgaaatc atagcaccat ttgcgccaat taatgtgtgc tgatgaaata ctgatggtaa
    12121 aactggcaac aaatcacgaa tcggacgtgc tgatgcaaat ataagttcat gaccatcatg
    12181 ttgtaattgt aacaatgtat caataatagt ctggtcaatt aatcggccgt caaaacaaag
    12241 cgtaccatca atatcaaaga caaatctcat caaatcactc caaacaatat aataccatga
    12301 ttatagcata agtaagttat gtgacgatat tagataatga ttatgatgta gggtaccttt
    12361 tgcgttacac acat
//
LOCUS       DAKTIO010000076        12364 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.076, whole genome shotgun sequence.
ACCESSION   DAKTIO010000076 DAKTIO010000000
VERSION     DAKTIO010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12364
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.076"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            431..631
                     /locus_tag="O0293_001531"
     CDS             431..631
                     /locus_tag="O0293_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCY0280418.1"
                     /translation="MNNGTVKWFNAEKGFGFIEQENGGDVFVHFSGIASDGYKTLEEG
                     QKVTFEITEGQRGDQAVNVQTV"
     gene            complement(758..1327)
                     /locus_tag="O0293_001532"
     CDS             complement(758..1327)
                     /locus_tag="O0293_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCY0280419.1"
                     /translation="MNLSKQIKKYRERDGYSQEYLAEKLYVSRQSISNWENDKSLPDI
                     HNLLMMCELFNVTLDDLVKGTIPFVPDIKAQRSLNLWTYVMLIFMTLAAILMGPLVVY
                     WNWTWGVTVAIILGIGFYASMKIEDLKKVHKMDNYDRIVAFMNGKDPSEVQTTKARNT
                     MTNALSIISVIGILSLIIFLSVYLANKFL"
     gene            complement(1587..1982)
                     /locus_tag="O0293_001533"
     CDS             complement(1587..1982)
                     /locus_tag="O0293_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HCY0280420.1"
                     /translation="MKLTLMKFFVGGFAVLLSYIVSVTLPWKEFGGIFATFPAVFLVS
                     MFITGMQYGDKVAVHVSRGAVFGMTGVLVCILVTWMMLHMTHMWLISIVVGFLSWFIS
                     AVCIFEAVEFIAQKRLEKHSWKAGKSNSK"
     gene            complement(2050..2403)
                     /locus_tag="O0293_001534"
     CDS             complement(2050..2403)
                     /locus_tag="O0293_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HCY0280421.1"
                     /translation="MFSIGSAILHFVIGGIAVALASIIADKVGGKLGGIIATMPAVFL
                     AAIIALALDHRGTQLVEMSMNLSTGAIVGILSCILTVFLTSLYIKHKGYRKGAIFTVV
                     CWFVISLAIFSIRHL"
     gene            complement(2856..3695)
                     /gene="noc"
                     /locus_tag="O0293_001535"
     CDS             complement(2856..3695)
                     /gene="noc"
                     /locus_tag="O0293_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HCY0280422.1"
                     /translation="MKKPFSKLFGLKNKDDIIGHIEEDRNSNVESIQIERIVPNRYQP
                     RQVFEPNKIKELAESIHEHGLLQPIVVRPIEEDMFEIIAGERRFRAIQSLNLPQADVI
                     IRDMDDEETAVVALIENIQRENLSVVEEAEAYKKLLEIGDTTQSELAKSLGKSQSFIA
                     NKLRLLKLAPKVLLRLREGKITERHARAVLSLSDSEQEALIEQVIAQKLNVKQTEDRV
                     RQKTGPEKVKAQNLRFAQDVTQARDEVGKSIQAIQQTGLHVEHKDKDHEDYYEIKIRI
                     YKR"
     gene            complement(3738..4457)
                     /gene="rsmG"
                     /locus_tag="O0293_001536"
     CDS             complement(3738..4457)
                     /gene="rsmG"
                     /locus_tag="O0293_001536"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCY0280423.1"
                     /translation="MTVEWLAEQLKEHNIQLTETQKQQFQTYYRLLVEWNEKMNLTSI
                     TDEHDVYLKHFYDSIAPSFYFDFNQPISICDVGAGAGFPSIPLKIMFPQLKVTIVDSL
                     NKRIQFLNHLASELQLQDVSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLP
                     LVKKGGQFVALKSSKGEEELEEAKFAISVLGGNVTETHTFELPEDAGERQMFIIDKKR
                     QTPKKYPRKPGTPNKTPLLEK"
     gene            complement(4457..6334)
                     /gene="mnmG"
                     /locus_tag="O0293_001537"
     CDS             complement(4457..6334)
                     /gene="mnmG"
                     /locus_tag="O0293_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCY0280424.1"
                     /translation="MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMP
                     CNPSVGGPAKGIVVREIDALGGQMAKTIDKTHIQMRMLNTGKGPAVRALRAQADKVLY
                     QQEMKRVIEDEENLHIMQGMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGE
                     IILGNMKYSSGPNHQLPSITLSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPG
                     DDVGRAFSFETTEYILDQLPCWLTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPS
                     IEDKFVRFNDKPRHQLFLEPEGRNTNEVYVQGLSTSLPEHVQRQMLETIPGLEKADMM
                     RAGYAIEYDAIVPTQLWPTLETKMIKNLYTAGQINGTSGYEEAAGQGLMAGINAAGKV
                     LNTGEKILSRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDMGY
                     ELGMISEERYARFNEKRQQIDAEIKRLSDIRIKPNEHTQAIIEQHGGSRLKDGILAID
                     LLRRPEMTYDIILELLEEEHQLNADVEEQVEIQTKYEGYINKSLQQVEKVKRMEEKKI
                     PEDLDYSKIDSLATEAREKLSEVKPLNIAQASRISGVNPADISILLIYLEQGKLQRVS
                     D"
     gene            complement(6401..7780)
                     /gene="mnmE"
                     /locus_tag="O0293_001538"
     CDS             complement(6401..7780)
                     /gene="mnmE"
                     /locus_tag="O0293_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCY0280425.1"
                     /translation="MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLND
                     VPSHTINYGHIIDPESKEVIEEVMVSVLRAPKTFTREDIIEINCHGGILTINRVLELT
                     MTYGARMAEPGEFTKRAFLNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLI
                     KKQRQSILEILAQVEVNIDYPEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIM
                     REGLSTVIVGKPNVGKSSMLNNLIQDNKAIVTEVAGTTRDVLEEYVNVRGVPLRLVDT
                     AGIRETEDIVEKIGVERSRKALSQADLILFVLNNNEALTQEDYTLYEVVKNEDVIVIV
                     NKMDLEQNIDINEVKDMIGDTPLIQTSMLKQEGIDELEIQIRDLFFGGEVQNQDMTYV
                     SNSRHISLLKQARQTIQDAIDAAESGVPMDMVQIDLTRTWEILGEIIGETASDELIDQ
                     LFSQFCLGK"
     gene            complement(7919..8272)
                     /gene="rnpA"
                     /locus_tag="O0293_001539"
     CDS             complement(7919..8272)
                     /gene="rnpA"
                     /locus_tag="O0293_001539"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCY0280426.1"
                     /translation="MLLEKAYRIKKNADFQRIYKKGHSVANRQFVVYTCNNKEIDHFR
                     LGISVSKKLGNAVLRNKIKRAIRENFKVHKSHILAKDIIVIARQPAKDMTTLQIQNSL
                     EHVLKIAKVFNKKIK"
     gene            complement(8393..8530)
                     /gene="rpmH"
                     /locus_tag="O0293_001540"
     CDS             complement(8393..8530)
                     /gene="rpmH"
                     /locus_tag="O0293_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCY0280427.1"
                     /translation="MVKRTYQPNKRKHSKVHGFRKRMSTKNGRKVLARRRRKGRKVLS
                     A"
     gene            9261..10622
                     /gene="dnaA"
                     /locus_tag="O0293_001541"
     CDS             9261..10622
                     /gene="dnaA"
                     /locus_tag="O0293_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCY0280428.1"
                     /translation="MSEKEIWEKVLEIAQEKLSAVSYSTFLKDTELYTIKDGEAIVLS
                     SIPFNANWLNQQYAEIIQAILFDVVGYEVKPHFITTEELANYSNNETATPKETTKPST
                     ETTEDNHVLGREQFNAHNTFDTFVIGPGNRFPHAASLAVAEAPAKAYNPLFIYGGVGL
                     GKTHLMHAIGHHVLDNNPDAKVIYTSSEKFTNEFIKSIRDNEGEAFRERYRNIDVLLI
                     DDIQFIQNKVQTQEEFFYTFNELHQNNKQIVISSDRPPKEIAQLEDRLRSRFEWGLIV
                     DITPPDYETRMAILQKKIEEEKLDIPPEALNYIANQIQSNIRELEGALTRLLAYSQLL
                     GKPITTELTAEALKDIIQAPKSKKITIQDIQKIVGQYYNVRIEDFSAKKRTKSIAYPR
                     QIAMYLSRELTDFSLPKIGEEFGGRDHTTVIHAHEKISKDLKEDPIFKQEVENLEKEI
                     RNV"
     gene            10900..12033
                     /gene="dnaN"
                     /locus_tag="O0293_001542"
     CDS             10900..12033
                     /gene="dnaN"
                     /locus_tag="O0293_001542"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCY0280429.1"
                     /translation="MMEFTIKRDYFITQLNDTLKAISPRTTLPILTGIKIDAKEHEVI
                     LTGSDSEISIEITIPKTVDGEDIVNISETGSVVLPGRFFVDIIKKLPGKDVKLSTNEQ
                     FQTLITSGHSEFNLSGLDPDQYPLLPQVSRDDAIQLSVKVLKNVIAQTNFAVSTSETR
                     PVLTGVNWLIQENELICTATDSHRLAVRKLQLEDVSENKNVIIPGKALAELNKIMSDN
                     EEDIDIFFASNQVLFKVGNVNFISRLLEGHYPDTTRLFPENYEIKLSIDNGEFYHAID
                     RASLLAREGGNNVIKLSTGDDVVELSSTSPEIGTVKEEVDANDVEGGSLKISFNSKYM
                     MDALKAIDNDEVEVEFFGTMKPFILKPKGDDSVTQLILPIRTY"
BASE COUNT         4061 a         2287 c         1791 g         4225 t
ORIGIN      
        1 cttattattt aaaacgtcgt gaagaaaaag gcaagtaagc acattccatc ggtatagaac
       61 attgctatga cctttcaagt cagattttaa tatcaacaag tagcgtttat cgctgcttgt
      121 tttttatgga ttgcttataa aatcatcatg tttaaacgat atgtcacttt gaaaactagc
      181 aaacaccata aaagactaca atttttttat ttttataaac ccctttaaat caacgtttct
      241 aaggttttta aaaatctttt ttaatttatt ttcaaaaaac actgtacatt atgccaatat
      301 gagcgtatag ttggtcttac gtaataaaag ctcgtgaatt aaattgtagt gtatttgttt
      361 agaatatcct cttttttagt tatgaatttg ttacaaatat taagtgcaaa agcacacgga
      421 ggttttctat atgaataacg gtacagtaaa atggtttaac gcagaaaaag gttttggttt
      481 catcgaacaa gaaaatggcg gagacgtatt cgtacatttc tcaggtatcg ctagcgatgg
      541 ctacaaaact ttagaagaag gtcaaaaagt tactttcgaa atcactgaag gtcaacgtgg
      601 agaccaagca gttaacgtac aaactgttta atcttacaac ataaaacgac tcattataaa
      661 tgaatcgctt tgattaccaa caaggttcta aatcttgttg gtattttttt atgctatttt
      721 ggataatata aataatatga gacgatacca cgatgattta taaaaactta tttgccaaat
      781 acacactaag gaaaattatg aggctgagta taccaattac tgatataata gaaagcgcat
      841 ttgtcatcgt atttctagct ttagtcgttt gtacttcact aggatctttt ccattcataa
      901 aagcaacaat tcgatcgtag ttgtccattt tatgcacttt ttttaaatct tctattttca
      961 tagatgcata aaaacctatt cccaaaatga ttgccaccgt tacaccccaa gtccaattcc
     1021 aataaacaac taaaggtccc attaaaattg cagctaatgt catgaaaata agcatcacat
     1081 atgtccataa gttaagactt cgttgcgctt taatatcagg tacaaatgga atggtccctt
     1141 ttactaaatc atctaaagtt acattgaaca attcacacat cattaataag ttatgtatgt
     1201 ctggtaagct tttgtcattt tcccaattag aaatactctg cctagataca tataactttt
     1261 cagcaagata ttcttgtgaa taaccatctc gttccctata ctttttaatt tgtttactca
     1321 gattcaatta catacgcctc cagtcaatta gtacaatcat tacaatcaga ctatcagata
     1381 aagttaaatc aagtcacctt tatgattgac taaatgtttt gacatacgac aacatcgggg
     1441 ttcgaagact cccttaagac agacctgtat gattacgcgc ttgtaatttt ctcaatgcca
     1501 acttgaattt cacttattaa tgatgcaact tttccatgct attcaatgct tgcaaagtta
     1561 gtcctagtta agagattacg ttcacactat ttactattcg attttccagc tttccaacta
     1621 tgcttttcta atcttttttg tgctataaat tctaccgctt caaaaataca tactgcactg
     1681 atgaaccagc ttaggaaacc aacaacaatg ctaatcaacc acatgtgcgt catatgtaac
     1741 atcatccatg taactaaaat acaaactaat acccctgtca taccaaacac tgcgccacga
     1801 cttacatgca cagcgacttt atcaccatat tgcatacctg taataaacat agacactaaa
     1861 aatactgccg gaaacgttgc aaatataccg ccaaattctt tccaaggtag tgttacagat
     1921 acaatataac ttaataatac tgcaaatccc cccacaaaaa atttcattaa tgttaatttc
     1981 atgatgttct gcctccaata atttattact taataactga atacaactaa ttatcacgca
     2041 ttttccaaac tataaatgtc taatactgaa tattgcgagg gaaatgacaa accaacaaac
     2101 aactgtgaat attgcgcctt tccgataacc tttatgctta atgtagagag atgtcaaaaa
     2161 tacagttaat atacaagaca gaataccgac aattgctcca gtactaagat tcatcgacat
     2221 ctccactaat tgcgtaccac gatgatctaa agctaatgcg ataatagccg caagaaagac
     2281 tgccggcata gtagctataa tacctcctaa cttaccacct accttatcag caataattga
     2341 agctaatgca acagcgatac caccaatgac aaaatgaaga attgcacttc caattgaaaa
     2401 catcttcaac gtacctttct ataattaatt caatacaaaa cgtatcattc acacaaagtg
     2461 taaaacttaa attttctact gtaaaggcaa ctcaagcgat gttcataaag ctttcatgat
     2521 actttcataa actagcaata aagggttcaa atgaaagtat atccagctca aattagggag
     2581 tgggacagaa atgatatttt cgcaaaattt atttcgtcgt cccaccccag caaggttgac
     2641 tagaattgaa aaaagcttgt tacaagcgca ttttcgttca gtcaactact gccaatataa
     2701 cttcgtagag catagaacat tgatttatgt accagcctga tcaacatata aatataaatt
     2761 tttatgtttc acgtaaaaca aaaaaaaaga caaagctgtt atgatcttag ctttgtcttt
     2821 tatgtgttag tcatcatgta tacgacatcc tactactaac gtttatatat tcgaattttt
     2881 atttcataat aatcttcatg atctttgtct ttatgctcaa catgtaatcc tgtttgttga
     2941 atcgcttgga tacttttgcc tacctcatct cgtgcttgag tgacatcttg tgcaaagcga
     3001 aggttttgtg ctttgacttt ttcgggcccc gttttttggc gtactctatc ttcagtctgt
     3061 ttcacattta gcttttgtgc aatgacttgc tcaatcaacg cttcttgttc gctatcagac
     3121 aatgataata ccgctctcgc atgacgttca gtaattttac cttctcttaa gcgaagtagt
     3181 actttcggcg ccaacttcaa taaacgcaac ttatttgcaa taaagctttg acttttacct
     3241 aaactttttg ccaattcact ttgcgttgta tcaccaattt ccaataattt cttataggct
     3301 tccgcttctt caacaacaga caaattttct ctttgaatat tctcaattaa tgcaacaaca
     3361 gccgtctctt catcatccat atcacgaata ataacgtctg cttgaggtaa atttagtgat
     3421 tgtattgctc taaatcggcg ctctccagca ataatttcaa acatatcttc ttcaatcggt
     3481 cttacaacaa taggttgtag taaaccatgt tcatgtattg attcagcaag ttctttaatt
     3541 ttatttggtt caaacacctg tcttggttga taacggttgg gaacgatacg ttcaatttga
     3601 atggattcaa cattactatt gcgatcttct tcaatatgtc caatgatgtc atctttgttt
     3661 ttcaaaccaa ataattttga aaaaggtttt ttcattatcc attcgctcct tttatgttag
     3721 ttgtaaagga ttatgcatta tttttcaagt aaaggagtct tattaggcgt ccctggtttt
     3781 cttggatact ttttcggcgt ctgtcttttt ttatcaataa tgaacatctg gcgctctcca
     3841 gcatcttctg gcaattcaaa ggtatgtgtt tctgtaacat taccacctaa cacactaatt
     3901 gcaaattttg cttcttctaa ttcttcttca ccttttgaag attttaatgc aacaaactgt
     3961 ccaccttttt taactagcgg taaacacaat tcacttaaca cggataatct agctactgct
     4021 cttgcagtaa caacatcata agactccctg tagacaccct taccaaatgt ttctgctcta
     4081 tcgtgtataa agctgacatc ctgtaattgt aattctgacg ctaaatggtt taaaaattga
     4141 atacgcttat ttaatgaatc aacaatcgtc acttttaact gcggaaacat tatttttaac
     4201 ggaatacttg gaaaaccagc tccagcgcct acatcacata tacttatagg ctgattaaaa
     4261 tcaaaataaa aactaggtgc aatggaatca taaaaatgtt tcaaatatac atcgtgttca
     4321 tctgtaatac ttgtcaaatt catcttttca ttccattcaa caagtaaacg ataatatgtt
     4381 tgaaactgtt gtttttgagt ctcagttaat tgaatattat gttcttttaa ttgttctgct
     4441 aaccattcta cagtcattag tcactcaccc tttggagttt accttgttct aagtaaatca
     4501 ataatataga tatgtctgct ggatttaccc ctgatattct agaagcttgt gcaatattta
     4561 aaggttttac ttctgataat ttttctcgcg cttcagtcgc caaactatca atcttactat
     4621 aatctaagtc ttctggaatt ttcttctctt ccatacgctt aactttctca acttgttgta
     4681 gtgatttatt gatataacct tcatattttg tttgtatttc tacttgttct tcaacatctg
     4741 cattcaattg atgttcttct tctaaaagtt ctaaaattat atcgtaagtc atttcaggtc
     4801 tgcgtaataa atcgatagct aaaataccat cttttaagcg agaaccacca tgttgttcaa
     4861 taatcgcttg cgtatgttcg tttggtttaa tacgaatatc tgataaacgc ttaatttccg
     4921 catcaatttg ctgacgtttt tcattaaaac gtgcatatct ttcttcagaa atcataccaa
     4981 gttcatatcc catatccgtc aatctcaaat cagcattatc atgacgtagt aacaaacgat
     5041 attctgcacg tgatgttagt aaacgataag gttcattagt acctttagtt acaagatcat
     5101 cgattaagac accaatatat gcatctgaac gacttaatat cttttcgcct gtgtttaaca
     5161 ctttacctgc agcgttaata cctgccatca atccttgtcc tgctgcttct tcataaccag
     5221 atgtaccatt aatttgacct gcagtatata agtttttaat cattttcgtt tcaagtgtag
     5281 gccataactg cgttggcaca atcgcatcat attcaattgc gtagccggca cgcatcatat
     5341 ctgctttttc aagacctggt atcgtctcta acatttgacg ttgcacatgt tcaggaagac
     5401 ttgtagacaa tccttgcaca tatacttcat ttgtattacg accttcaggc tctaagaaaa
     5461 gttgatgtcg cggcttatca ttaaatcgaa caaatttatc ttcaattgaa gggcaataac
     5521 gtggcccggt tcctttaatc atccctgaat acattgcaga tagatgtaaa ttatcatcga
     5581 taactttgtg tgtttcagca ttagtatacg ttagccaaca tggcaattga tctaatatat
     5641 attctgttgt ttcaaagctg aatgcacgac ctacatcgtc acctggttgt atttcagtct
     5701 tcgaatagtc aattgttttt gaatttacac gcggtggtgt acctgtttta aaacgaacaa
     5761 tatcaaaacc aagttctctt aaattgtctg ataatgtgat tgatggtaat tggtgatttg
     5821 gtccacttga atacttcata ttacctaaaa tgatttcacc acgtaaaaat gttcccgttg
     5881 taataattac tgctttagat aaatactctg taccaatatt tgtacgtaca cctttaactt
     5941 cattatcttc tataataagt tcgtctacca taccttgcat tatatgcaaa ttttcttcat
     6001 cttcaatcac gcgtttcatt tcttgttgat aaagtacttt atctgcttgc gctcttagtg
     6061 ctcttacagc aggtccttta cctgtattta acattctcat ttgaatgtgt gttttatcga
     6121 ttgtttttgc catttgtcca cctaaagcat caatttcacg aacaacgata cctttagctg
     6181 gtccacctac agatgggtta catggcataa atgcaatatt atctaaattt attgttagca
     6241 ttaatgtttt agcaccacgt cttgcagatg ctaaacctgc ttctacacct gcatgtcccg
     6301 cacctataac gattacatca tattcttgaa ccacaatata aacctcctta tttgatatct
     6361 tactagcctt cttaagacgg tattccgtct atttcaatta ctatttacct aagcagaatt
     6421 gactgaataa ctgatcgatg agttcatcac ttgcagtctc accaataatt tctcctaata
     6481 tttcccaagt tctagttaaa tcaatttgta ccatatccat aggcacacca gattctgctg
     6541 catcaatcgc atcttgtatc gtttgtcttg cttgttttaa taatgaaata tgtcttgaat
     6601 tagaaacata agtcatatct tgattttgta cttctccacc aaagaacaaa tctcgaattt
     6661 gtatttctaa ttcatcaata ccttcttgtt ttaacattga agtttgaatt aatggcgtat
     6721 cacctatcat atctttaact tcattaatat ctatgttttg ctctaaatcc attttattaa
     6781 caattacgat tacatcttca tttttaacca cttcatataa tgtgtaatct tcttgagtca
     6841 atgcttcgtt attgtttaat acaaataaaa ttaagtctgc ttggctaaga gcctttctag
     6901 agcgttcaac accaatcttc tctactatat cttctgtctc acgtatacca gcagtatcaa
     6961 ctaatcttaa tggcacgcca cgaacattga cgtactcttc taagacatct ctagtagtac
     7021 ctgctacctc agttacaatc gctttattat cttgtattaa attatttaac atcgatgatt
     7081 tacctacgtt tggtttacca acaataactg tagataaacc ttcacgcata attttaccct
     7141 gcgcaccggt atctaataaa cgattaattt cctgtttgat ttctttagac tgctctaaaa
     7201 gaaattcagt agtcgcatct tcaacatcat cgtattcagg ataatcaata ttcacttcca
     7261 cttgagcgag tatctctaat atagattgac gttgtttttt gattaagtca cttagacgac
     7321 cttcaatttg attcatcgca actttagaag ctctatctgt cttcgagcga ataaagtcca
     7381 taactgcttc agcttgagat aaatcaatac gaccatttaa aaaggcacgt tttgtaaatt
     7441 cacctggctc agccattcta gcgccatatg tcatagtaag ttccagcact ctattaatcg
     7501 ttaaaatacc accatgacaa ttaatttcta taatatcttc gcgtgtaaat gtttttggcg
     7561 ctcttaacac agacaccata acttcttcaa tcacttcttt agactctgga tcaataatat
     7621 gaccgtaatt aatcgtatgt gatggaacat catttaaaag atgttttcct ttatataatt
     7681 tgtcagcaat ttcaacggct tgcggtccag acaatcgaac aattccaatt gccccttcac
     7741 ccattggtgt tgaaatactc gtaattgtat ctaaatccat attgctactc gcctccttca
     7801 acgatgtgaa tacattttaa agtaagttat tataacccta aggtcagtct tacgtttgtc
     7861 tgaggtaaga cttcgggatg tgttgagtgg ttaatgtttt ccttccccta ccctatcctt
     7921 acttaatctt tttattaaaa actttggcaa ttttaagtac gtgctcaaga ctattctgta
     7981 tttgtaaagt cgtcatatct ttagctggct gtcttgctat tacaataata tctttggcca
     8041 atatatgcga cttatgtact ttgaaatttt cacgtattgc tcttttaatc ttgtttctta
     8101 acactgcatt acctagtttt ttagaaacac taatacctaa gcgaaaatgg tctatttctt
     8161 tattattaca agtgtataca acaaattgtc tgttggctac agaatgacct tttttatata
     8221 ttctctgaaa atctgcattc tttttaattc ggtaagcttt ttccaataac atcactcgct
     8281 tatttatcgt ttttatttga agctatattt aaacttctat tgagcttata acataaattt
     8341 ctatttattc ttaatttaaa cgaaaaaaaa gatcactgat gagtcagtga tcttatgcag
     8401 ataaaacttt acggccttta cgacgacggc gcgctaaaac tttacggcca ttttttgtgc
     8461 tcatgcgttt tctgaaacca tgaactttac tatgtttacg tttatttggt tgataagtac
     8521 gttttaccat gcaaaacacc tccatcttta tacagttatc taatatacca gattatgcag
     8581 actctgatca ctttaataga taagattttg cgtttcttag attaaaattc tcgtattagc
     8641 tcattgatta tctagtcata attcaagcaa ctactacaat ataacaaaat cctatttata
     8701 acgcaagttc attttatact actgctcaat ttttttactt ttatcgatta aagatagaaa
     8761 tacacgatgc gagcaatcaa atttcataac atcaccatga gtttggtccg aagcatgagt
     8821 gtttacaatg ttcgaacacc ttatacagtt cttatacata ctttataaat tatttcccaa
     8881 actgttttga tacactcact aacagatact ctatagaagg aaaagttatc cacttatgca
     8941 catttatagt tttcagaatt gtggataatt agaaattaca cacaaagtta tactattttt
     9001 agcaacatat tcacaggtat ttgacatata gaggactgaa aaagtataat tgtgtggata
     9061 agtcgtccaa ctcatgattt tataaggatt tatttattga tttttacata aaaatactgt
     9121 gcataactaa taagcaagat aaagttatcc accgattgtt attaacttgt ggataattat
     9181 taacatggtg tgtttagaag ttatccacgg ctgttatttt tgtgtataac ttaaaaattt
     9241 aagaaagatg gagtaaattt atgtcggaaa aagaaatttg ggaaaaagtg cttgaaattg
     9301 ctcaagaaaa attatcagct gtaagttact caactttcct aaaagatact gagctttaca
     9361 cgattaaaga tggtgaagct atcgtattat cgagtattcc ttttaatgca aattggttaa
     9421 atcaacaata tgctgaaatt atccaagcaa tcttatttga tgttgtaggc tatgaagtta
     9481 aacctcactt tattactact gaagaattag caaattatag taataatgaa actgctactc
     9541 caaaagaaac aacaaaacct tctactgaaa caactgagga taatcatgtg cttggtagag
     9601 agcaattcaa tgcccataac acatttgaca cttttgtaat cggacccggt aaccgctttc
     9661 cacatgcagc gagtttagct gtggccgaag caccagccaa agcgtacaat ccattattta
     9721 tctatggagg tgttggttta ggaaaaaccc atttaatgca tgccattggt catcatgttt
     9781 tagataataa tccagatgcc aaagtgattt acacatcaag tgaaaaattc acaaatgaat
     9841 ttattaaatc aattcgtgat aacgaaggtg aagctttcag agaaagatat cgtaatatcg
     9901 acgtcttatt aatcgatgat attcagttca tacaaaacaa ggtacaaaca caagaagaat
     9961 ttttctatac ttttaatgaa ttgcatcaga ataacaagca aatagttatt tcgagtgatc
    10021 gaccaccaaa ggaaattgca caattagaag accgattacg ttcacgcttt gaatgggggc
    10081 taattgttga tattacgcca ccagattatg aaactcgaat ggcaattttg cagaagaaaa
    10141 ttgaagaaga aaaattagat attccaccag aagctttaaa ttatatagca aatcaaattc
    10201 aatctaatat tcgtgaatta gaaggtgcat taacacgttt acttgcatat tcacaattat
    10261 taggaaaacc aattacaact gaattaactg ctgaagcttt aaaagatatc attcaagcac
    10321 caaaatctaa aaagattacc atccaagata ttcaaaaaat tgtaggccag tactataatg
    10381 ttagaattga agatttcagt gcaaaaaaac gtacaaagtc aattgcatat ccgcgtcaaa
    10441 tagctatgta cttgtctaga gagcttacag atttctcatt acctaaaatt ggtgaagaat
    10501 ttggtgggcg tgatcatacg accgtcattc atgctcatga aaaaatatct aaagatttaa
    10561 aagaagatcc tatttttaaa caagaagtag agaatcttga aaaagaaata agaaatgtat
    10621 aagtaggaaa ctttgggaaa tgtaatctgt tatataacag cactaatgat aacaatcatt
    10681 ttttacattt ctatatgcta atgtggcaag atgagcaaaa ctcattttgt ggataatgtt
    10741 taaaagtcat acacaccata cacaagttat caacatgtgt ataacttcgc caaatctatg
    10801 tttttaagac ttatccacca atccacagca cctactacta ttactaagaa cttaaaacct
    10861 atataattat atataaacga ctggaaggag ttttaattaa tgatggaatt cactattaaa
    10921 agagattatt ttattacaca attaaatgac acattaaaag ctatttcacc aagaacaaca
    10981 ttacctatat taactggtat caaaatcgat gcgaaagaac atgaagttat attaactggt
    11041 tcagactctg aaatttcaat agaaatcact attcctaaaa ctgtagatgg cgaagatatt
    11101 gtcaatattt cagaaacagg ctcagtagta cttcctggac gattctttgt tgatattata
    11161 aaaaaattac ctggtaaaga tgttaaatta tctacaaatg aacaattcca gacattaatt
    11221 acatcaggtc attctgaatt taatttaagt ggcttagatc cagatcaata tcctttatta
    11281 cctcaagttt ctagagatga cgcaattcaa ttgtcggtaa aagtgcttaa aaacgtgatt
    11341 gcacaaacaa attttgcagt gtccacctca gaaacacgcc cagtactaac tggtgtgaac
    11401 tggcttatac aagaaaatga attaatatgc acagcgactg actcacaccg cttggctgta
    11461 agaaagttgc agttagaaga tgtttctgaa aacaaaaatg tcatcattcc aggtaaggct
    11521 ttagctgaat taaataaaat tatgtctgac aatgaagaag acattgatat cttctttgct
    11581 tcaaaccaag ttttatttaa agttggaaat gtgaacttta tttctcgatt attagaagga
    11641 cattatcctg atacaacacg tttattccct gaaaactatg aaattaaatt aagtatagac
    11701 aatggggagt tttatcatgc gattgatcgt gcctctttat tagcgcgtga aggtggtaat
    11761 aacgttatta aattaagtac aggtgatgac gttgttgaat tgtcttctac atcaccagaa
    11821 attggtactg taaaagaaga agttgatgca aacgatgttg aaggtggtag cctgaaaatt
    11881 tcattcaact ctaaatatat gatggatgct ttaaaagcaa tcgataatga tgaggttgaa
    11941 gttgaattct tcggtacaat gaaaccattt attctaaaac caaaaggtga cgactcggta
    12001 acgcaattaa ttttaccaat cagaacttac taaaaataaa tataaataaa ggatgacgtg
    12061 attaattaaa acgtcatcct ttattttttg gcaaaaataa ttctaggtgc gtatgtaaaa
    12121 taaatttggc agcattttaa acagcaaata aaagacgcca attaaattta tgacaaatgt
    12181 atccaaaatt taataagtgt gcttatatgc cctttaaatt taaaatttta atagtcaata
    12241 acaagttgaa tataaaagtt aaacgccgtt aaatagcgtt aaaaaattga aaatgacagt
    12301 attgccaaaa aataagaatt aattatttat atgtaaacgg tttctacctc tattttaaat
    12361 gaaa
//
LOCUS       DAKTIO010000077        12331 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.077, whole genome shotgun sequence.
ACCESSION   DAKTIO010000077 DAKTIO010000000
VERSION     DAKTIO010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12331
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.077"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(1..1580)
                     /locus_tag="O0293_001543"
                     /pseudo
     CDS             complement(1..1580)
                     /locus_tag="O0293_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500098.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1573..2502)
                     /locus_tag="O0293_001544"
     CDS             complement(1573..2502)
                     /locus_tag="O0293_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCY0280430.1"
                     /translation="MKKFIGSVLATTLILGGCSTMENESKKDTKTETKSVPEEMEASK
                     YVGQGFQPPAEKNAIEFAKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGVEVYVH
                     CEDHGIVFNASLPLYKDAIHQKGSMRSNDNGDDMSMMVGTVLSGFEYRAQKEKYDNLY
                     KFFKENEKKYQYTGFTKEAINKTQNVGYKNEYFYITYSSRSLKEYRKYYEPLIRKNDK
                     EFKEGMERARKEVNYAANTDAVATLFSTKKNFTKDNTVDDVIELSDKLYNLKNKPDKS
                     TITIQIGKPTINTKKAFYDDNRPIEYGVHSKDE"
     gene            complement(2747..4498)
                     /gene="srrB"
                     /locus_tag="O0293_001545"
     CDS             complement(2747..4498)
                     /gene="srrB"
                     /locus_tag="O0293_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SrrB"
                     /protein_id="HCY0280431.1"
                     /translation="MMSRLNSVVIKLWLTIILIVTTVLILLSIALITFMQYYFTQETE
                     NAIREDARRISSLVEQSHNKEEAIKYSQTLIENPGGLMIINNKHRQSTASLSNIKKQM
                     LNEVVNNDHFDDVFDKGKSVTRNVTIKEKGSSQTYILLGYPTKAQKNSHSKYSGVFIY
                     KDLKSIEDTNNAITIITIITAVIFLTITTVFAFFLSSRITKPLRRLRDQATRVSEGDY
                     SYKPSVTTKDEIGQLSQAFNQMSTEIEEHVDALSTSKNIRDSLINSMVEGVLGINESR
                     QIILSNKMANDIMDNIDEDAKAFLLRQIEDTFKSKQTEMRDLEMNARFFVVTTSYIDK
                     IEQGGKSGVVVTVRDMTNEHNLDQMKKDFIANVSHELRTPISLLQGYTESIVDGIVTE
                     PDEIKESLAIVLDESKRLNRLVNELLNVARMDAEGLSVNKEVQPIAALLDKMKIKYRQ
                     QADDLGLNMTFNYCKKRVWSYDMDRMDQVLTNLIDNASRYTKPGDEIAITCDENESED
                     ILYIKDTGTGIAPEHLQQVFDRFYKVDAARTRGKQGTGLGLFICKMIIEEHGGSIDVK
                     SELGKGTTFIIKLPKPE"
     gene            complement(4479..5204)
                     /gene="srrA"
                     /locus_tag="O0293_001546"
     CDS             complement(4479..5204)
                     /gene="srrA"
                     /locus_tag="O0293_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator SrrA"
                     /protein_id="HCY0280432.1"
                     /translation="MSNEILIVDDEDRIRRLLKMYLERESFEIHEASNGQEAYELAME
                     NNYACILLDLMLPEMDGIQVATKLREHKQTPIIMLTAKGEETNRVEGFESGADDYIVK
                     PFSPREVVLRVKALLRRTQSTTVEQSEPHARDVIEFKHLEIDNDAHRVLADNQEVNLT
                     PKEYELLIYLAKTPNKVFDREQLLKEVWHYEFYGDLRTVDTHVKRLREKLNRVSSEAA
                     HMIQTVWGVGYKFEVKSNDEPAK"
     gene            complement(5337..6074)
                     /locus_tag="O0293_001547"
     CDS             complement(5337..6074)
                     /locus_tag="O0293_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCY0280433.1"
                     /translation="MTKELERLQKRIANSGYTSRRKAETLISEGKVKVNGTTVTELGT
                     KVKPSDTIEVEGIKIELEDKIYILFHKPTQVITSVSDDRGRTVVTDYFKDIEARIYPV
                     GRLDYDTSGLLLLTNDGEFTNLMTHPRYQIKKKYVAKLKGYLMREEVKALEKGIELED
                     GMTQPAEVKVKKQDKDKNTTLVEITITEGRNRQVRRMFEHFGHQVTKLSRIEYGPLNV
                     VGLNAGEGRVLTPHEVKVMRHLAEHGK"
     gene            complement(6067..6609)
                     /gene="scpB"
                     /locus_tag="O0293_001548"
     CDS             complement(6067..6609)
                     /gene="scpB"
                     /locus_tag="O0293_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HCY0280434.1"
                     /translation="MDNHGILESLLFTAGDEGLDEKQLLEILDMSKDQLVELIENYSS
                     HGLMIQRFGMTYVLTTKKEAATYIEQLIEQKSQMKLSQAAMEVLSIIAYNQPLSRSDI
                     ELIRSINSDGAVKTLIAKGLVEAKVVNEQRSQQLITTDLFLNVFGISNIEDLPTTEED
                     DEEMDAFFSNLVNQKGENND"
     gene            complement(6602..7333)
                     /locus_tag="O0293_001549"
     CDS             complement(6602..7333)
                     /locus_tag="O0293_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HCY0280435.1"
                     /translation="MYEVKLDAFNGPLDLLLHLIQKFEIDIYDIPMQALTEQYMQYVH
                     AMKQLEINIASEYLVLASELLMIKSKMLLPQSTSDMDVDDDPREDLVGRLIEYQNYKE
                     YTAILNDMKEERDFYFTKRPTDLSHLETDESWDPNHTIDLTELIVAYQRVKNRVELNT
                     PKSVEIRKETFTIQQATEQVTSRLKDKDHFNFFSLFTFSEPIEQVVTHFLAILEMSKA
                     GIINIEQQRNFEDINIIRGVNYHFG"
     gene            7425..7931
                     /locus_tag="O0293_001550"
     CDS             7425..7931
                     /locus_tag="O0293_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HCY0280436.1"
                     /translation="MQQALINFYYQFHTKQHYFLCHDILEDAWKAENNYSKQDAVVSL
                     ILFATACYHYRRNNLKGAYKSFNKSKEIIQNAKDRDALYLNLNDYQLLIEQQIAKLNA
                     AKPFSSVILPITPVFERIIKANYPDYDYNQETATDPFIVDHHMRRDRSEVIAAKEEAI
                     QLRKHRRN"
     gene            complement(8009..8896)
                     /gene="xerD"
                     /locus_tag="O0293_001551"
     CDS             complement(8009..8896)
                     /gene="xerD"
                     /locus_tag="O0293_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCY0280437.1"
                     /translation="METIIEEYLRFIQIEKGLSSNTIGAYRRDLKKYQDYMTEHHISH
                     IDFIDRQLIQECLGHLIDQGQSAKSIARFISTIRSFHQFAIREKYAAKDPTVLLDSPK
                     YDKKLPDVLNVDEVLALLETPDLNKINGYRDRTMLELLYATGMRVSELIHLELENVNL
                     IMGFVRVFGKGDKERIVPLGDAVIEYLTTYIETIRPQLLKKTVTEVLFLNMHGKPLSR
                     QAIWKMIKQNGVKANIKKTLTPHTLRHSFATHLLENGADLRAVQEMLGHSDISTTQLY
                     THVSKSQIRKMYNQFHPRA"
     gene            complement(8945..9394)
                     /locus_tag="O0293_001552"
     CDS             complement(8945..9394)
                     /locus_tag="O0293_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCY0280438.1"
                     /translation="MEERLNRVKQQLQQSSYKLTPQREATVRVLIENEKDHLSAEDVY
                     LKVKDKAPEIGLATVYRTLELLAELKVVDKINFGDGVARFDLRKEGAKHFHHHLVCME
                     CGRVDEIDEDLLPEVENRVENEFNFKILDHRLTFHGVCETCQAKGKG"
     gene            complement(9499..10041)
                     /locus_tag="O0293_001553"
     CDS             complement(9499..10041)
                     /locus_tag="O0293_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCY0280439.1"
                     /translation="MDLNEKTIDRTVIYNGKIVDVEIHTVTLPNGETSTRELVYHNGA
                     VAVCAVTPKKEVVLVKQYRKPVEKPLLEIPAGKLEDDEDRVEAAKRELEEETGYIAKE
                     LTHVVDMYGSPGFCDEQLSIYFTDNLEEGTVHLDEDEFVEVIKVPIENVKSMLMNKEI
                     EDAKTIIALQHLLLNYNHSK"
     gene            10123..11031
                     /locus_tag="O0293_001554"
     CDS             10123..11031
                     /locus_tag="O0293_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCY0280440.1"
                     /translation="MQKNILKSGISLSELGLGCMSLGTDLKKAEQIIDCAVENGITYF
                     DTADMYDKGINESVVGKALLKYQQRDDIFIGTKVGNRLTKDGSTTWDPSKSYIKEAVK
                     GSLKRLGIDHIDLYQLHGGTIDDPLDETISAFDELKQEGIIRAYGISSIRPNVIDYYL
                     KHSQIETIMSQFNLIDNRPESLLDAIHNNDVKVLARGPVSKGLLTSNSVNVLDNKFKD
                     GIFDYSHDELGETIASIKEIESNLSALTFSYLTSHDVLGSIIVGASSVDQLKENIENY
                     HTKVSLDQIKTARARVKDLEYTNHLV"
     gene            complement(11247..11495)
                     /locus_tag="O0293_001555"
     CDS             complement(11247..11495)
                     /locus_tag="O0293_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280441.1"
                     /translation="MNCQSESVVRLCVRYAEQLSVFEEFTVLDILGDISVDQISDGTL
                     YYTCEKFKLLVLQGNVLGVQIITNNDESTCEVKYRKMF"
     gene            complement(11511..12266)
                     /locus_tag="O0293_001556"
     CDS             complement(11511..12266)
                     /locus_tag="O0293_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCY0280442.1"
                     /translation="MKGQHFIVTGGTSGLGLSIVRKLLENKVHVTLLVRDVDKATRIF
                     EQELGKTINVIPCDLNDLKSIQALQFEDNTSFDGFIYSAGLGYFKSISDHSFSEMIET
                     YQLNLISFNVLYTVLRPYLTSNAHIVGISSQAAFSTQANAAHYGASKAGFYALMNALR
                     LESPNLHIMTVNVGPIDTPFHQKADPSMKYAKKMGEIMLDANQLAEDIIYGIKTKQLE
                     INRPKWMHHALKMYQIAPRFFERCFPKLFKNKA"
BASE COUNT         3791 a         2278 c         1657 g         4605 t
ORIGIN      
        1 taaataatta gaaggtataa ctagaccctg taccatattt tcgccgctac aattaatttg
       61 aaaatttcca tctaaaattg ttgcatcttg ttgtttcata atacttctaa tatctgcaat
      121 ttgcctacca taaatatcat tttgattttt tatttggtct atattctgtt tcactacttt
      181 caaatgtttc atcatttctt cagatactcc atctctgaag tcgtgatcta tatttttgaa
      241 aatttctaaa atttcattat ctatactatc atacactttt tctataaaag attttatacc
      301 tttaaacaac tcattaattc tttcttttaa tgcatccaat gcaaaatcag gtaataagtg
      361 tttaacagca ctaatacttt ctattgtttc atctgcaact tcttcaagtg agtttatttt
      421 actaattaaa gttctttcca tttcttctaa ctgaaataag ttaatcttat ccttaaatcc
      481 ttctgataat tgtttctttc tatctgcaaa atttttattt tcattttctg agatgttaaa
      541 actttcattt aaaaagatta cgcattctgc taacatacca cttgtttcac cagtaatcag
      601 tttactcaac gcatcaagat tttctaaatt aagtttaatt aaagttcctc ttccagaacg
      661 tgcaatcgaa tctcctgtcc aaacatttat tggaattcgc ccatccatat ctaatgttat
      721 gttaatagtc ttttttactt ttttccattt ttaatttctg tatcttttac cgactttatt
      781 ttgattagtg gtacagtatc gtatgtgtta tcttttctat ttaacttcct tttataacct
      841 acatggctgt ctattaaagt atctaacctg ggcacaccat cattaatgtt aacgcgtttt
      901 cctggcatat ctttgatgaa tggatcttgt aaccatgtta ataaatcgtt ggtactatta
      961 aaactaatca tattatcaaa gcgtggtcta gcaaatttct gccaagcagc ataaggaatc
     1021 attgctgagt cagtagcaac aactttttca ttcggatgtt tcgctccttg atattttgct
     1081 cctgcaccgc cttccgaatt accgccatcc gccacaatgg ttttgttttt gaagttactt
     1141 gattccattc tatatttttt taaaaaatca ctatttgata atctatctgc atcttctaac
     1201 tttattttat attgatttga taattgatct gtttgcttta aataatctgt gctttcatta
     1261 tcattattca ttaatttagc attttggagc caatcatctc caaaccccga tgattttaat
     1321 ggattatttg gattaattgc ctcattagat gttccttgat aaattattgt ctgttgacca
     1381 gttggtttac ttttatcatc taataactca tatattttaa tatcagcagc accattcaaa
     1441 ttactattct tatcatcatt ataactgtct acttgtttaa acctttttcc attaacttta
     1501 aattcttttt ttatatcaat attttgataa acccagtaac tacttaattc tgttaaatcc
     1561 ctatcattaa ttttattcat ctttactgtg caccccatat tctattggac gattatcatc
     1621 ataaaaggct ttcttagtat taatagtggg tttccctatt tgtattgtga ttgtagattt
     1681 atctggttta ttttttaaat tatataattt atcacttagt tcgattacat catctactgt
     1741 attgtcttta gtaaagtttt tcttagtaga aaaaagtgta gcaacagcat ctgtatttgc
     1801 agcgtaattc acttcttttc ttgctcgttc cattccttct ttaaattctt tatcattttt
     1861 tcgaatcagt ggttcataat actttcgata ttcttttaaa cttctagaag agtatgtaat
     1921 ataaaaatat tcatttttat atccgacatt ttgtgtcttg ttaattgcct cttttgtaaa
     1981 gcctgtatat tgatatttct tttcattttc tttgaagaat ttatataagt tatcatactt
     2041 ttctttttgc gctcgatatt caaagccact cagcactgta cccaccatca tactcatatc
     2101 atcaccattg tcattactgc gcattgatcc tttttgatgg atggcatctt tgtacaaagg
     2161 tagacttgca ttaaatacaa tgccatgatc ttcacaatgc acataaactt ctacaccatc
     2221 atctttacct acaacatttg tagctttaac ttttagtcca aagttatctt taaagaattg
     2281 ttcacctact ttttcaaatt ctttacgatg cttcttcgca aattcaatcg catttttttc
     2341 tgcaggcggt tgaaagcctt ggcctacata ttttgaagct tccatttctt ctggtacaga
     2401 ttttgtttct gtttttgtgt ctttttttga ttcattttcc atcgtggaac atccccctaa
     2461 aattaatgtc gtagctaaaa ctgatccaat gaattttttc atatgacacc tcgaataatt
     2521 taattaatac aatatatcta tattttaccc tatttttata atcaaataaa taactttatc
     2581 aaactttata tttttgtata aattagtaat atatttaata gttgatattc gcaaaaaccc
     2641 taagttctaa tccacataca aaattcccca catcaatcaa aaaacgcatg ctataaatta
     2701 gagtctctaa tcaataacat gcgttctgaa ataactatat tcaattttat tctggttttg
     2761 gtagtttaat aataaatgtt gtgcctttcc ctaattcgct tttaacatct atggaaccac
     2821 catgctcttc gataatcatt ttacaaatga acaaacctaa accggtacct tgtttacctc
     2881 gcgttctcgc tgcatcaact ttataaaaac gatcaaatac ttgttgtaaa tgttctggtg
     2941 caatgcctgt acctgtatct ttaatgtata aaatatcttc gctttcattt tcatcacaag
     3001 taattgcaat ttcatctcca ggtttcgtat aacgtgatgc attatcaatt aagttcgtta
     3061 gtacttggtc catgcgatcc atatcataac tccaaacacg cttcttacaa taattaaaag
     3121 tcatatttag acctaaatca tcagcttgtt ggcgatactt aattttcatc ttatctagta
     3181 acgctgcaat aggctgaact tctttattta cggataaccc ttcagcatcc atgcgtgcga
     3241 catttaacaa ttcattaact aaacgattta aacgtttcga ttcatcaagg acaatggcaa
     3301 gcgattcttt tatttcatcc ggttctgtaa caataccatc tacaattgat tcagtataac
     3361 cttgaagtaa tgatatcggt gtacgtaatt catgtgatac attagcaatg aaatctttct
     3421 tcatttgatc tagattgtgc tcattagtca tatcacgaac tgtcacaaca acaccacttt
     3481 tacctccctg ttcaatcttg tcgatatagc ttgtggtcac aacaaagaat cgtgcattca
     3541 tttctaaatc gcgcatttca gtttgttttg atttaaaagt atcttctatt tgtcttaata
     3601 agaaagcttt agcatcttca tcaatattgt ccataatatc attcgccatc ttattagata
     3661 agataatttg tcgactctca ttaataccta ggacaccttc taccatagag ttaattaagc
     3721 tgtctctaat atttttagat gtggataatg cgtcgacatg ctcttcgatt tctgtactca
     3781 tctgattaaa tgcctgcgat aattgaccaa tttcatcttt cgttgtgaca gaaggtttat
     3841 aagagtaatc cccttcagat acacgtgtag cttggtctct taaacgtctt aaaggttttg
     3901 taattcttga cgataagaaa aacgcaaaga ctgttgtaat tgttaagaaa ataacagccg
     3961 taattatggt gataatcgta atagcattat ttgtatcttc gattgatttc aagtctttat
     4021 atataaagac tccactatat ttgctatgac tattcttctg tgcttttgtt ggatagccta
     4081 acaaaatata tgtttgagat gagccctttt ctttaatcgt tacatttcga gtaacagatt
     4141 tacctttatc aaacacatcg tcaaaatggt cgttgttgac tacttcattc aacatttgct
     4201 ttttaatatt agaaagtgaa gccgttgatt gacgatgttt attatttata atcatcaacc
     4261 caccaggatt ttcaattaat gtttgactat attttattgc ttcttcttta ttatgtgatt
     4321 gttcgaccag tgaacttata cgtctagcat cttctcttat ggcattttcg gtttcttgtg
     4381 tgaaatagta ttgcataaag gtaattaaag caatacttaa taaaattaaa actgtcgtca
     4441 ctattaaaat aatagttaac cacagtttaa ttacgacact atttagccgg ctcatcatta
     4501 gatttaacct caaatttata cccaacgccc cagactgttt gaatcatatg cgcagcttca
     4561 ctagacacac gatttaactt ttctctaagt cgtttaacat gagtatcaac tgttcttaaa
     4621 tcaccataga attcataatg ccaaacttct tttaataatt gttcacggtc aaatacttta
     4681 tttggtgttt tagctaaata tattaataat tcgtactctt taggagtcaa attaacttct
     4741 tgattatcag caagtacgcg atgtgcatca ttatctattt ctaaatgttt aaattcaatc
     4801 acatcacgtg cgtgaggttc gctttgttct acagttgtag attgcgttct tcttagaagt
     4861 gctttaactc ttaagactac ttctcttggt gaaaatggtt tgacgatata atcatctgca
     4921 ccagattcaa aaccttcaac acggtttgtt tcttcacctt tagcagtcaa cataataatc
     4981 ggtgtttgtt tatgttcacg caatttagtt gccacctgga taccatccat ttcaggcaac
     5041 attaaatcta gtagtatgca agcataatta ttctccattg caagttcata agcctcttgg
     5101 ccattacttg cttcatggat ttcaaaagat tctctttcta aatacatttt aagtaatctt
     5161 ctgattctat cctcatcatc tacgataagt atttcgttcg acatacaggt catacctccc
     5221 acacatgctt ttctttacaa aagtattata tcacaggaaa gcgcatacag ttattaaaaa
     5281 cctaaaattt tgtgaaaaaa ttctcaaact ttgtgataaa tacatatttt atttaattat
     5341 ttcccatgtt ctgctaaatg acgcatcact tttacttcat gaggcgtcaa tacacgtcct
     5401 tcaccagcat tcaaaccgac aacatttaaa ggcccatatt caatacgaga cagtttcgtc
     5461 acttgatgac caaaatgttc gaacattctt ctgacttggc gattacgacc ttctgtaatt
     5521 gtaatttcaa ccaatgttgt gtttttatct ttatcttgtt tcttaacttt cacttcagcc
     5581 ggttgcgtca taccatcttc taattcaata cctttttcta gcgctttcac ttcttctctc
     5641 attaaataac cttttaattt cgcaacatat tttttcttaa tttgatatct tggatgtgtc
     5701 attaaattag taaattcacc atcatttgtg agtaataaca atccagaagt atcatagtct
     5761 aaacgaccaa caggataaat acgtgcttct atatctttaa agtaatctgt aaccactgta
     5821 cgtcctctat catcagatac acttgttatc acttgagttg gtttatggaa taaaatgtaa
     5881 attttgtctt ctagttctat tttaatacct tcaacttcaa tcgtatctga tggcttcact
     5941 tttgttccta attcagtgac agtcgtacca ttcactttca cttttccttc agaaattaaa
     6001 gtttctgcct tacgtcttga agtataaccg ctattagcta tacgtttttg taatcgttct
     6061 aattctttag tcattatttt ctcctttttg attgactaga ttactgaaaa aagcatccat
     6121 ttcttcatcg tcttcttcag ttgtcggcaa atcttctata ttggaaatac caaatacatt
     6181 taaaaataaa tcagtagtaa ttaactgttg gctacgctgt tcattaacca ctttagcctc
     6241 aactagtcct ttggcaatca atgtcttaac tgcaccatct gaattgatac tacgaattaa
     6301 ttcaatatca cttcttgata atggctggtt ataagcaata attgatagta cttccattgc
     6361 tgcttgtgat aatttcattt gtgacttttg ttcaattaat tgttcaatat acgttgccgc
     6421 ttctttttta gtcgttaaaa catacgtcat tccaaatcgt tgtatcatta atccatgtga
     6481 tgaataattt tcaattaatt caacgagttg gtctttcgac atatctaata tttctaatag
     6541 ttgtttttca tctaaacctt catcgccagc tgtaaataaa agcgactcta atataccatg
     6601 attatccaaa atggtagttc actcctctaa taatgttaat atcttcaaaa ttacgttgct
     6661 gctcaatatt aattattcct gcttttgaca tctctaaaat agctaaaaag tgagtgacta
     6721 cttgttcaat tggctcagaa aacgtaaaca gactaaagaa gttaaaatga tctttatctt
     6781 tcaatctcga tgtcacttgt tctgtagctt gttgaatggt aaatgtctct tttcggattt
     6841 caacagattt aggtgtattt aactcaactc tatttttaac tctttgataa gctacaatta
     6901 attcagttaa atcaatcgta tgatttggat cccaagattc atctgtttcc aaatgagata
     6961 aatctgtcgg tctttttgta aaataaaaat ctctttcttc tttcatgtca tttaaaatag
     7021 cagtatattc tttataattt tgatattcta ttaaacgccc aactaaatct tcccgtgggt
     7081 catcatcaac atccatatct gatgttgatt gtggtaatag catcttactt ttaatcatta
     7141 agagttctga cgctaatact aggtattcac ttgcaatatt aatttcaagc tgtttcattg
     7201 catgaacgta ctgcatatac tgctctgtta atgcttgcat aggaatatca taaatatcta
     7261 tttcaaattt ttggataaga tgcagcaata aatctaatgg tccattgaaa gcatctaatt
     7321 taacttcata cataatatct acctcgtatt gcgtcatatg tgcataattc tctaaaatat
     7381 tatagcatga aaataactta cttttaaatt ggggtgataa caaaatgcaa caggcactga
     7441 taaatttcta ttatcaattt catactaagc aacattattt cctatgtcat gacattttag
     7501 aagatgcatg gaaagctgaa aataactaca gtaagcaaga tgcagttgtt agtttaatct
     7561 tgtttgcaac tgcttgttac cattatcgcc gcaataattt aaaaggcgcc tataagtctt
     7621 ttaataaatc taaagaaata atacaaaatg ctaaagatag agatgcgtta taccttaatt
     7681 taaatgatta tcagttgtta attgagcaac agattgcaaa attgaatgca gcaaagcctt
     7741 tttcatctgt tatattacca ataactccag tctttgaacg catcatcaag gccaactatc
     7801 cagactatga ttataatcag gaaacagcaa cagatccttt tattgtagac catcacatgc
     7861 gccgtgatcg ttcagaagtg attgcagcaa aagaagaagc gattcaatta agaaaacata
     7921 gacgtaatta atcaaccaat ttaaaaaagc cacaatagca cagacatttt tagtcatgtg
     7981 tattgtgact tttgagttat tgttcacttt atgctctagg atgaaattgg ttatacattt
     8041 ttctaatttg agatttcgaa acatgtgtat agagttgggt agtagatatg tcagagtgac
     8101 ctaacatctc ttgcactgct cttaaatctg cgccattttc caataaatgt gtcgcaaaag
     8161 agtggcgtaa cgtatgtggc gttaacgtct ttttaatgtt tgcctttaca ccattttgtt
     8221 taatcatttt ccatattgct tgtcgtgata aaggtttacc atgcatattt aaaaataaga
     8281 cttcagtaac agtctttttt aaaagttgcg gtctaatcgt ttcaatataa gtagttaagt
     8341 actcaatgac tgcgtcgcct aatggtacaa ttctttcttt atcgccttta ccaaatacgc
     8401 gtacaaatcc cattattaag ttcacgtttt ctaactctaa atgtatcaat tcagatacac
     8461 gcattcccgt tgcgtacaga agttctaaca tcgtacgatc acgatatcca ttaattttat
     8521 ttaaatctgg cgtttctaat aaagccaaca cttcgtcaac atttaaaacg tcaggcaatt
     8581 ttttgtcata ttttggtgaa tctaataata ccgttggatc tttcgccgca tatttttctc
     8641 ttatagcaaa ttgatgaaaa ctacggattg ttgaaataaa tcgcgcaata gatttagcag
     8701 attgcccttg gtctattaaa tgccccaaac actcttgaat taattgtcga tctataaaat
     8761 caatatgcga gatatgatgt tcagtcatat aatcttgata ctttttcaaa tcacgtctat
     8821 aagcaccaat tgtgttagaa cttagtcctt tttcaatttg tataaaacgc aaatattctt
     8881 caataattgt ttccataacc acacctctaa aaaaagcgaa ggttaattta acctacgcaa
     8941 tttactatcc tttaccttta gcttggcacg tttcacacac accatggaaa gttaaacgat
     9001 gatctaaaat tttaaaattg aactcatttt caactcgatt ttcaacttct ggtaacaaat
     9061 cttcatcgat ttcatctaca cgaccacatt ccatacatac taaatgatgg tggaaatgtt
     9121 ttgcgccttc ttttcttaaa tcaaaacgag cgacgccatc accaaagtta attttgtcga
     9181 caacttttag ttcagctaac aactctaacg ttctgtatac tgtcgccaag ccaatttcag
     9241 gcgctttatc ttttactttc agatatacgt cttcagcact tagatgatct ttttcatttt
     9301 caattagaac tctaacagta gcttcgcgtt gtggcgttag cttatatgat gattgttgta
     9361 attgttgctt aacgcgattt aatcgttctt ccaacgatgt ccactcccct actaataatt
     9421 aaaatcatta caaattattt caaacttcac aattaaaact aacagttttc tcaataaaat
     9481 gcaagctttt ctcatttgtt atttagaatg attataattt aataataggt gttgcaatgc
     9541 aatgatagtc ttcgcatctt ctatttcttt attcattagc atcgatttaa cattctcaat
     9601 cggaacttta atgacttcga caaattcatc ttcatctaag tgaactgtac cttcttctaa
     9661 attatccgta aaatatattg ataattgttc atcgcaaaaa cctggtgaac catacatatc
     9721 tacaacatgt gttaactctt tggcaatata tcctgtttct tcttctaatt cacgttttgc
     9781 cgcttcgact ctatcttcat catcttctaa tttaccagct ggaatttcta gcaatggttt
     9841 ttctactggt ttacgatact gtttcactaa tacgacttct tttttaggtg tcactgcaca
     9901 aacagcaact gcaccattat gataaactaa ttctcttgtt gacgtttcac cgtttggtaa
     9961 tgtcactgta tgaatttcta catctacaat tttgccatta taaataactg ttcgatcaat
    10021 tgttttttca tttaaatcca ttataatcac gttcctttaa attcatatta tatattgata
    10081 cactatgctt gttagctaaa gtgtatcgaa aggagaacag acatgcaaaa aaatatatta
    10141 aaaagtggta tttcattatc tgaattaggt ctaggttgca tgagtttagg tacagattta
    10201 aaaaaagccg aacaaattat agattgtgct gttgaaaatg gtatcactta ttttgataca
    10261 gcagatatgt acgataaagg tataaatgaa tcagttgttg gtaaggcact tcttaaatat
    10321 caacaacgcg atgatatttt tattggtaca aaagtaggca atcgtttaac aaaagatggc
    10381 agtacaacat gggatccgag taaatcctat attaaagagg cagttaaagg ttcactaaag
    10441 cgtttaggta tcgatcatat cgatttatat caacttcatg gcggaaccat tgatgaccca
    10501 ttagacgaaa caataagcgc atttgatgaa ttgaaacaag aaggaattat acgtgcttac
    10561 ggtatttctt ctattcgccc aaatgtaatt gattattatt taaaacatag tcaaatcgaa
    10621 acgataatgt ctcaattcaa tttgattgat aatcgtccag aatcattatt agatgcaatt
    10681 cacaacaatg atgttaaagt attggcaaga ggacctgtgt ctaaaggatt attaacttca
    10741 aacagtgtta atgtgctcga caataaattt aaagatggta tttttgatta ttctcatgat
    10801 gaattgggtg aaacaatagc ctctattaaa gaaattgaaa gtaatttatc tgcattgaca
    10861 tttagttatt taacatcaca tgacgtgctt ggttccatca ttgtaggtgc aagtagcgtc
    10921 gaccaattaa aagaaaatat tgaaaactat catactaaag ttagtttaga tcagattaaa
    10981 acagcaagag ctcgtgtaaa ggatttggaa tataccaatc atttagtgta gaagtcattt
    11041 tcagtaataa aaacagcagc atgaggcgtt tcattataaa aatgccttac tgctgttgtt
    11101 tatgtacaat tcgctataat ttatgattat gattactcac ttatgataga aattaaagcg
    11161 ttgtcctcac gcatcagtat ttagtaattt cgccttgcgg cattgcctta agcaaacttc
    11221 tgccacttca tctcttaata attttattaa aacatctttc tatatttcac ttcgcatgtt
    11281 gattcatcat tattagttat tatttgtaca cccagcacat ttccttgcaa cacaagtagt
    11341 ttgaattttt cacaagtata atataatgta ccgtctgaaa tttggtctac agaaatatcg
    11401 cctaaaatat ccagcactgt aaattcttca aatactgata gttgttccgc atatcgtaca
    11461 caaagtctta ccacactctc cgattgacag ttcattgcca tcccacctat ttatgcttta
    11521 tttttaaata atttagggaa acatcgttca aaaaatctag gcgcaatttg atacattttc
    11581 aacgcatgat gcatccattt aggccgatta atttccaatt gttttgtttt aatgccataa
    11641 atgatatctt ctgcaagctg attagcatca agcataattt cccccatctt tttagcatac
    11701 ttcattgatg ggtcggcttt ttgatgaaaa ggtgtatcaa tcgggccaac attaactgtc
    11761 atgatatgta agtttggtga ctctagtctt aaagcattca ttaatgcata aaaccctgct
    11821 ttcgatgccc cataatgtgc agcatttgct tgtgtggaaa atgcagcttg acttgaaata
    11881 cctacaatat gtgcgttaga tgttaaatat ggtctcaaca cagtatataa aacattaaaa
    11941 ctaattaaat taagctgata cgtttcaatc atttctgaaa aactatggtc tgaaatagat
    12001 ttgaaataac ctaaacctgc actataaatg aatccatcga atgatgtatt gtcttcaaat
    12061 tgcagtgcct gtatcgactt caaatcattt aagtcacaag gaataacatt tatagttttc
    12121 cccaattcct gttcaaagat tctagttgct ttatcaacat cacgcaccaa caacgttaca
    12181 tgcactttat tttctagtaa ctttcggaca atcgataaac ctaaaccact cgtaccacca
    12241 gtcactataa aatgttgtcc tttcatcaat taaccttcct tttcaattat atagaatgca
    12301 atttatcaac tttacataat tgagacaagt t
//
LOCUS       DAKTIO010000078        12213 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.078, whole genome shotgun sequence.
ACCESSION   DAKTIO010000078 DAKTIO010000000
VERSION     DAKTIO010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12213
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.078"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..144
                     /locus_tag="O0293_001557"
     CDS             <1..144
                     /locus_tag="O0293_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCY0280443.1"
                     /translation="VKRELGFVLMALNIRKIAAQRAVHYKIHIKKADFHQIINRNQLF
                     YIA"
     gene            complement(280..1182)
                     /gene="cvfB"
                     /locus_tag="O0293_001558"
     CDS             complement(280..1182)
                     /gene="cvfB"
                     /locus_tag="O0293_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HCY0280444.1"
                     /translation="MALDKDIVGSIEFLEVVGLQGSTYLLKGPNGENVKLNQSEMNDD
                     DELEVGEEYSFFIYPNRSGELFATQNMPDITKDKYDFAKVLKTDRDGARIDVGLPREV
                     LVPWEDLPKVKSLWPQPGDYLLVTLRIDRENHMYGRLASESVVENMFTPVHDDNLKNE
                     VIEAKPYRVLRIGSFLLSESGYKIFVHESERKAEPRLGESVQVRIIGHNDKGELNGSF
                     LPLAHERLDDDGQVIFDLLVEYDGELPFWDKSSPEAIKEVFNMSKGSFKRAIGHLYKQ
                     KIINIETGKIALTKKGWSRMDSKE"
     gene            1329..2930
                     /locus_tag="O0293_001559"
     CDS             1329..2930
                     /locus_tag="O0293_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0280445.1"
                     /translation="MLQVTDVSLRFGDRKLFEDVNIKFTEGNCYGLIGANGAGKSTFL
                     KILSGELDSQTGHVSLGKNERLAVLKQDHYAYEDERVLDVVIKGHERLYEVMKEKDEI
                     YMKPDFSDEDGIRAAELEGEFAEMNGWNAEADAANLLSGLGIDPTLHDKKMAELENNQ
                     KIKVLLAQSLFGEPDVLLLDEPTNGLDIPAISWLEDFLINFDNTVIVVSHDRHFLNNV
                     CTHIADLDFGKIKVYVGNYDFWYQSSQLAQKMAQEQNKKKEEKMKELQDFIARFSANA
                     SKSKQATSRKKQLEKIELDDIQPSSRRYPFVKFTPEREIGNDLLIVQNLSKTIDGEKV
                     LDNISFTMNPNDKAILIGDSEIAKTTLLKILAGEMEPDEGSYKWGVTTSLSYFPKDNS
                     EFFEGVNMNLVDWLRQYAPEDEQTETFLRGFLGRMLFSGEEVKKKASVLSGGEKVRCM
                     LSKMMLSSANVLLLDEPTNHLDLESITAVNDGLKSFKGSIIFTSYDFEFINTIANRVI
                     DLNKQGGVSKEIPYEEYLQEIGVLK"
     gene            3790..4995
                     /locus_tag="O0293_001560"
     CDS             3790..4995
                     /locus_tag="O0293_001560"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCY0280446.1"
                     /translation="MVTRSVLKFGGSSVSDFTKIKRIAEMLKERVNQDEQLIVVVSAM
                     GNTTDQLMTNVSTLTKAPKQQELALLLTTGEQQTVSYLSMVLNDIGMNAKAMTGYQAG
                     IKTIGHHLKSKIAQINPQTFEQAFQENDILVVAGFQGINEHQELTTLGRGGSDTTAVA
                     LAVSNQIPCEIYTDVDGVYATDPRLLPKAKRLDIVSYEEMMEMSALGAGVLETRSVEL
                     AKNYNIPLYLGKTLSNVKGTWIISNEEILEKKAVTGVALDKHMMHVTISYPLPDNQLL
                     TQLFTELEEGAVNVDMISQIVNLDGLQLSFTIKDSDFHQISMILETLKNQYEALAYKI
                     NEHYVKISLIGSGMRDMSGVASKAFLTLIENNIPFYQTTTSEISISYVIDDFNGQQAV
                     EKLYDAFNI"
     gene            5059..6048
                     /locus_tag="O0293_001561"
     CDS             5059..6048
                     /locus_tag="O0293_001561"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCY0280447.1"
                     /translation="MTKLAVVGATGLVGTKMLETLNRKNIPFDELVLFSSARSAGQEV
                     EFQGKTYTVQELTDARASEHFDYVLMSAGGGTSEHFAPLFEKAGAIVIDNSSQWRMAE
                     DIDLIVPEVNEPTFTRGIIANPNCSTIQSVVPLKVLQDAYGLKRVAYTTYQAVSGSGM
                     KGKKDLAEGVNGKAPEAYPHPIYNNVLPHIDVFLENGYTKEEQKMIDETRKILNAPDL
                     KVTATCARVPVQDSHSVEIDVTLDKETTAEDIKALFDQDDRVVLVDNPENNEYPMAIN
                     STNKDEVFVGRIRRDDSLENTFHVWCTSDNLLKGAALNAVQVLEQVMRLKGAN"
     gene            6050..6937
                     /locus_tag="O0293_001562"
     CDS             6050..6937
                     /locus_tag="O0293_001562"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCY0280448.1"
                     /translation="MTHLFEGVGVALTTPFTNNKVNIEALKTHVNFLLENNAQAIIVN
                     GTTAESPTLTTDEKERILKTVIDLVDKRVPVIAGTGTNDTEKSIQASIQAKALGADAI
                     MLITPYYNKTNQRGLVKHFEAIADAVKLPVVLYNVPSRTNMTIEPETVEILSQHPYIV
                     ALKDATNDFEYLEEVKKRIDTNSFALYSGNDDNVVEYYQRGGQGVISVIANVIPKEFQ
                     ALYDAQQSGLDIQDQFKPIGTLLSALSVDINPIPIKALTSYLGFGNYELRLPLVSLED
                     TDTKVLRETYDTFKAGENE"
     gene            6934..7656
                     /locus_tag="O0293_001563"
     CDS             6934..7656
                     /locus_tag="O0293_001563"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCY0280449.1"
                     /translation="MKILLIGYGAMNQRVARLAEEKGHEIVGVIENTPKATTPYQQYQ
                     HIADVKGADVAIDFSNPNLLFPLLDEDFHLPLVVATTGEKEKLLNKLDELSQNMPVFF
                     SANMSYGVHALTKILAAAVPLLDDFDIELTEAHHNKKVDAPSGTLEKLYDVIVSLKEN
                     VTPVYDRHELNEKRQPQDIGIHSIRGGTIVGEHEVLFAGTDETIQITHRAQSKDIFAN
                     GAIQAAERLVNKPNGFYTFDNL"
     gene            7683..8402
                     /gene="dapD"
                     /locus_tag="O0293_001564"
     CDS             7683..8402
                     /gene="dapD"
                     /locus_tag="O0293_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HCY0280450.1"
                     /translation="MVQHLTAEEIIQYISDAKKSTPIKVYLNGNFEGITYPESFKVFG
                     SEQSKVIFCEADDWKPFYEAYGSQFEDIEIEMDRRNSAIPLKDLTNTNARIEPGAFIR
                     EQAIIEDGAVVMMGATINIGAVVGEGTMIDMNATLGGRATTGKNVHVGAGAVLAGVIE
                     PPSASPVIIEDDVLIGANAVILEGVRVGKGAIVAAGAIVTQDVPAGAVVAGTPAKVIK
                     QASEVQDTKKEIVAALRKLND"
     gene            8545..9696
                     /locus_tag="O0293_001565"
     CDS             8545..9696
                     /locus_tag="O0293_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCY0280451.1"
                     /translation="MNELEFVTKHRRHLHQHPELSLHEFETTAYIKAFLDSLNIKYDC
                     PLETGVIAYLEGNGSHTIAYRADIDALPILEENDVPYRSQSDHVMHACGHDGHTTALM
                     LFVQRCKDMQDAGQLPQNVVFIFQPAEETGGGANRLIKAGAFDKYPIEAVFGIHVNPF
                     ADEGIAVIRDEEITASATEYRFFLTGLSSHVADKEQGHSCGEALQHVLTQISQIQQFH
                     LNGLKRNIVHIGHFKAGEAINTVPSNGYLEGTIRTYDIDDLTIVKNQMHKIAESVKLL
                     FNVDCEVKFAEGYPPTINSPKLRTQIEDALIKADLNVYDKPTPFLFGEDFSFYGQQLA
                     PAYFVFIGTRNEDKGFVTGLHTSHLNFDEKVLINVVNFYENLLNNYKEV"
     gene            9701..10786
                     /locus_tag="O0293_001566"
     CDS             9701..10786
                     /locus_tag="O0293_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCY0280452.1"
                     /translation="MTATWSVNKKIFLQNAITVKNNQPLMAVVKNNAYHYDLEFAVTQ
                     FIHAGIDTFSTTSLREAIQIRQLAPDATIFLMNAVYEFDLVREHQIHMTLPSLTYYYN
                     HKNDLAGIHVHLEFENLLHRSGFKDLNEIKEVLKDHHHNQNAKMIISGLWTHFGYADE
                     FDVSDYNVERSQWMEIVEALLSEGYQFDLIHAQNSASFYREGQILLPHHTHARVGIAL
                     YGSRPYSSLNQHDIVQSLTLKAHVIQVREVQAGDYCGYSFAFEVTKNNTKLAVVDIGY
                     GDGILRTRAKHEALINGKRYPIRALMMSHMFVEVDGNVHAQDEVILYNNDIRIDEYTF
                     KGVGANSEQLSAMNHDSLKKEYISNDC"
     gene            10776..12041
                     /gene="lysA"
                     /locus_tag="O0293_001567"
     CDS             10776..12041
                     /gene="lysA"
                     /locus_tag="O0293_001567"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCY0280453.1"
                     /translation="MTVKYNQNGELTMDGISLKTIAQSFGTPTIVYDELQIREQMRRY
                     HRAFKDSGLKYNISYASKAFTCIQMVKLVAEEDLQLDVVSEGELYTALEAGFEPSRIH
                     FHGNNKTKHEIRYALENNIGYFVIDSLEEIELIDRYANDTVQVVLRVNPGVEAHTHEF
                     IQTGQEDSKFGLSIQYGLAKKAIDKVQQSKHLKLKGVHCHIGSQIEGTEAFIETAKIV
                     LRWLKEQGIQVELLNLGGGFGIKYVEGDESFPIESGIKDITDAIKSEIKVLGIDAPEI
                     GIEPGRSIVGEAGVTLYEVGTIKEIPEINKYVSIDGGMSDHIRTALYDAKYQALLVNR
                     NEEADDSVTIAGKLCESGDIIIKDAKLPSSVKRGDYLAILSTGAYHYSMASNYNQMQK
                     PSVFFLKDGKAREVIKRQSLRQLIINDTK"
BASE COUNT         4252 a         1919 c         2247 g         3795 t
ORIGIN      
        1 gttaaacgag agctaggttt tgtattaatg gcacttaata taaggaaaat agcagctcaa
       61 cgagctgtac attataaaat acatatcaaa aaagctgatt tccatcaaat aattaataga
      121 aatcagcttt tttacattgc ctaagaactt aatgtcccag cctattcgtt tgaattattt
      181 aattttttac tttgaaagga atgtttaaaa aactacttca tctgtccgat ttaatattga
      241 ataaaaaaaa cacgcatcct acgacacgtg taaaaatgat tattcttttg agtccattcg
      301 actccaacct tttttagtta aagcgatttt acctgtttct atattaataa tcttctgttt
      361 atataagtga ccgattgcac gtttgaatga acctttactc atattgaata cttctttaat
      421 cgcttcaggg cttgatttgt cccagaatgg taattcacca tcatattcaa ctagtaaatc
      481 aaagatgact tggccgtcat cgtctaaacg ttcatgtgca agtggtaaaa atgaaccatt
      541 taactcacct ttatcattat gcccgataat tctaacttga acagattcac ctaatcttgg
      601 ttcagcttta cgttctgatt catgtacgaa aattttgtaa cctgattcgc ttaataaaaa
      661 gctaccaatt cgtaatacgc ggtaaggttt ggcttcaatg acttcgtttt ttaaattatc
      721 gtcgtgtaca ggtgtaaaca tattttctac aacagattca ctcgctaaac gtccatacat
      781 atgattctca cggtcaattc gtaatgtaac tagcaaataa tcaccaggtt gtggccatag
      841 tgatttcact tttggtaaat cttcccatgg tactaacact tcacggggta atccaacatc
      901 tatacgtgcc ccatcgcgat ccgttttaag tactttagca aagtcatatt tatctttcgt
      961 aatatcaggc atattttgag ttgcaaataa ttcacctgaa cggtttggat aaatgaagaa
     1021 actatattct tcacctactt ctaattcatc atcatcgttc atttctgatt ggtttaactt
     1081 tacgttttca ccgtttggtc cttttaaaag gtaagttgaa ccttgtaacc ctactacttc
     1141 aaggaattct atagaaccta ctatatcttt gtctaatgcc atgttattct ccttcgattc
     1201 ttgtattctt tattattata acatgtgtga gtcattctat cattacaact tatatgaggt
     1261 cgaaactgtg acgataatat gatataattc ttccgttaga atatcgaatg aaaaaaagga
     1321 gaaaatgcat gttacaagta actgatgtga gtttacgttt tggagatcgt aaactatttg
     1381 aagatgtaaa tattaaattt acagaaggta attgttatgg attaattggt gcgaatggtg
     1441 caggtaaatc aacattctta aaaatattat ctggtgaatt agattctcaa acaggacatg
     1501 tttcattagg gaaaaatgaa cgtctagctg ttttaaaaca ggaccactat gcttatgaag
     1561 atgaacgcgt gcttgatgtt gtaattaaag gtcacgaacg tctttatgag gttatgaaag
     1621 aaaaagatga aatctatatg aagccagatt tcagtgatga agatggtatc cgtgctgctg
     1681 aacttgaagg tgaatttgca gaaatgaatg gttggaatgc tgaagctgat gctgctaacc
     1741 ttttatctgg tttaggtatc gatccaactt tacacgataa aaaaatggct gaattagaaa
     1801 acaaccaaaa aattaaagta ttattagcgc aaagtttatt cggtgaacca gacgtactat
     1861 tactggatga gcctactaac ggtctcgata ttccagcaat cagttggtta gaagatttct
     1921 taattaactt tgataatact gttatcgtag tatcgcatga ccgtcatttc ttaaataatg
     1981 tatgtactca tatcgctgat ttagacttcg gtaaaattaa agtttatgtt ggtaactatg
     2041 atttttggta tcaatctagt cagttagctc aaaagatggc tcaagaacaa aacaagaaaa
     2101 aagaagaaaa aatgaaagag ttacaggact ttattgctcg tttctcagct aacgcttcta
     2161 aatctaaaca agcaacaagt cgtaaaaaac aactcgagaa aattgaatta gatgatattc
     2221 aaccatcatc aagaagatat cctttcgtta aattcacacc tgagcgcgaa atcggtaatg
     2281 acttactaat cgttcaaaat ctatctaaaa cgattgacgg tgaaaaagta ttagataata
     2341 tttcattcac aatgaatcca aatgataaag caattttaat tggggatagt gaaattgcga
     2401 aaaccacatt gcttaaaata ttagccggtg aaatggaacc agacgaaggt tcatataaat
     2461 ggggtgtaac aacgtcatta agttacttcc ctaaagataa ctcagaattc tttgagggcg
     2521 ttaatatgaa ccttgtagat tggttaagac aatacgcacc agaagatgag caaactgaaa
     2581 catttttacg cggcttctta ggccgtatgc tatttagtgg agaagaagtt aagaaaaaag
     2641 ctagtgtact ttcaggtgga gaaaaagtac gttgtatgct aagtaaaatg atgttatcaa
     2701 gtgcaaacgt tcttttactt gatgaaccaa caaaccactt agacttagaa agtattactg
     2761 ctgttaatga tggacttaaa tcattcaaag gttctattat ctttacttca tatgactttg
     2821 aatttatcaa tacaatcgca aaccgtgtta tcgatttaaa taaacaaggt ggcgtttcaa
     2881 aagaaattcc atatgaagaa tatttacaag aaatcggcgt tttaaaataa acataatatg
     2941 tagaatttct tttcgaaaat ctctgtattg aggcccgacc aacttgcaca ttattgtaag
     3001 ttggcttact gtcaccttct atattgggac ttattccgca acttgtattg tttgtagaaa
     3061 ttaggaatcc aatttctcta tgtgttgcac cgacaaggat tgaaaaaagc tagctacaaa
     3121 gtcattttca ttcagtcaac tactgtcaat ataacattgc agggcctagg gcattgattc
     3181 atgtccgaga ctatattcaa tttaatagac attaactggg cgtctcgatg ctaatattta
     3241 tagcatcgag gtgtcctttt tattttattt tttaacacat cagcatagtg aacacccctt
     3301 ctctaaatta caaatacagt tatcaacgca ttaaaagagt acatttattt aatgtgcata
     3361 ttgattatgc tataagtatt ttgaatttta tttttcaaaa agtctcatta tcaccacctt
     3421 ttaaaatata agcaacgcta taacttgaat tttcaggtta ttccaaaaaa tctaaattat
     3481 cggtttacat tcatatttgt catatgtata atgaacacat accaatttaa taatattttg
     3541 atgaggcgca tcaatcatga gtaaagttta gattactgtc tgctaacagc taaatttgaa
     3601 agggtgcgat gccgaagcaa ttataatagc agttataatt tgttggactt tttggttaag
     3661 agctgagagt ttgtcattat ttaaaaataa tggagtgcat cacttgtata tagattaaga
     3721 gcaagttcgc attccgaact tgttcttttt ttattattgt gtgcccttcc taacaattag
     3781 gaggattata tggtaacaag aagtgtgttg aaatttggcg gatcatccgt cagtgatttt
     3841 acaaaaataa aaaggatcgc tgaaatgtta aaggagcgag tcaatcaaga tgaacaatta
     3901 attgtcgttg taagtgctat gggtaacaca acagatcaat taatgacgaa tgtatcaacc
     3961 ttgactaaag caccaaaaca acaagaactg gcattattat tgacaaccgg agagcaacaa
     4021 actgtatctt atttatcaat ggtattaaat gatatcggta tgaatgccaa agcaatgact
     4081 ggctatcaag cgggtattaa aaccattggc catcatttaa aaagtaaaat tgctcaaatt
     4141 aatcctcaaa catttgaaca agcctttcaa gaaaacgata ttttagtagt tgctggattt
     4201 caaggcatca atgaacatca ggaattaaca actttaggca gaggtggttc tgatacgacc
     4261 gctgtggcac ttgctgttag taatcaaata ccttgtgaaa tttataccga cgttgatggt
     4321 gtgtatgcca ctgacccaag acttttacca aaggctaaac gactagacat cgtctcatat
     4381 gaagaaatga tggaaatgag cgctttaggt gctggtgtac ttgaaacaag aagtgttgaa
     4441 ttagctaaaa actataatat ccctttatat ttaggaaaaa ctttatcgaa cgtgaaagga
     4501 acatggatta tatcaaatga agaaatatta gagaaaaaag cagttactgg tgtggctttg
     4561 gataaacata tgatgcatgt aacaattagt tatcccctac ctgacaatca gctacttacc
     4621 caactattta cggaacttga agaaggtgct gtaaatgttg atatgatttc acaaatcgtc
     4681 aacttggatg ggctacaact atccttcacg attaaagata gtgattttca tcaaatttct
     4741 atgattcttg aaacattaaa gaatcaatat gaagcattag cttataaaat caatgagcat
     4801 tatgtcaaaa tttcattaat tggctcaggc atgcgtgata tgtcaggtgt ggcatcaaaa
     4861 gcatttttga cattaattga aaataatata cctttctacc aaacaacaac atctgaaata
     4921 agtatttcat acgtcattga tgattttaat gggcaacaag cggtagaaaa actatatgac
     4981 gcatttaaca tttaatggta aaatgattgt taaaatattc taaaaattgg aaattattat
     5041 aaaatggagt gacaagttat gacaaagtta gcagttgtgg gtgcaacagg attagtaggt
     5101 acaaaaatgt tggagacatt aaatcgtaaa aatattcctt tcgatgaatt agtattattt
     5161 tcatcagcac gttctgcagg gcaagaagtt gaatttcaag gaaaaacata tacagttcaa
     5221 gaattaactg atgctcgtgc aagtgaacat ttcgattatg tattaatgag tgctggtggc
     5281 ggtacaagcg aacactttgc cccacttttt gaaaaagctg gtgcaatcgt tatagacaat
     5341 tcaagtcaat ggcgtatggc agaagatatt gatttaatcg ttccggaagt caatgaacct
     5401 acatttacaa gaggtatcat tgccaatcca aactgctcta cgattcaatc tgttgtacct
     5461 ctaaaagtat tgcaagatgc ttatggttta aaacgagtgg catatacaac atatcaagct
     5521 gtatcaggtt cagggatgaa aggtaagaaa gatttagctg aaggtgtaaa tggtaaagca
     5581 ccagaagcat atccacatcc aatttataat aatgtgttac cgcatattga tgtgttttta
     5641 gaaaacggat atacaaaaga agaacaaaaa atgattgatg agacgagaaa aattttaaat
     5701 gcgccagact taaaagtaac agcaacatgc gcacgtgtgc ctgttcaaga tagtcatagt
     5761 gttgaaattg atgtaacgct tgacaaagaa acaacagcag aagatattaa agcgttattt
     5821 gatcaagatg accgcgttgt tttagtagac aatccagaga acaatgaata tccaatggca
     5881 atcaattcta ctaataaaga tgaagtgttt gttggccgta tacgtagaga tgattcatta
     5941 gaaaatactt tccatgtatg gtgtacatca gacaatttat taaaaggtgc tgcattaaat
     6001 gctgtacaag tattggaaca agttatgcgt ttaaaaggag cgaattaaaa tgacacattt
     6061 atttgagggt gttggcgttg cacttacaac cccttttaca aataacaaag ttaatattga
     6121 agctttgaaa acacacgtta attttttact agaaaataat gcccaagcaa tcatcgttaa
     6181 tggaactact gctgagagcc ctactttaac aacagatgaa aaagaacgca ttctaaaaac
     6241 agttattgat cttgtagata aacgtgttcc tgtcatagca ggaactggca ctaatgatac
     6301 tgaaaagtca atccaagctt caatccaagc taaagcctta ggggctgatg caattatgtt
     6361 aattacgccc tactacaaca aaacgaacca acgtggttta gtcaaacact ttgaagcgat
     6421 tgcagatgct gtgaaattac cagtcgtgct gtacaatgtt ccttcaagaa cgaacatgac
     6481 aattgaacca gaaactgtag aaatattaag tcaacatcct tatatagttg ctttaaaaga
     6541 tgctacgaat gattttgagt atttagaaga agtgaaaaag cgcattgata caaattcatt
     6601 tgcattatat agtggcaatg atgacaacgt cgtcgaatac tatcaacgtg gcggtcaagg
     6661 ggttatctct gttattgcca atgtcattcc taaagaattt caagcgttat acgatgctca
     6721 acaaagtgga ttagatattc aagatcaatt taaaccaatc ggcacactgt tatcagcttt
     6781 atcagttgat attaacccaa ttcctattaa agctctaaca agttatttag gatttggaaa
     6841 ttatgaatta cgtctaccat tggttagcct agaagataca gatactaaag tgcttcgtga
     6901 aacatatgac acatttaaag cgggtgaaaa tgagtgaaaa tattactaat tggctatggc
     6961 gcaatgaatc aacgcgttgc tagattagca gaagaaaaag gacatgaaat cgttggggtc
     7021 attgaaaata caccgaaagc aacaacgcca tatcaacaat atcaacatat tgcagatgtt
     7081 aaaggtgccg atgttgcaat agatttttca aatccaaatc tgcttttccc tttattagat
     7141 gaagattttc atttgccatt agttgtggca acaactggcg agaaagaaaa actacttaat
     7201 aagttagatg aattgagtca aaatatgcct gtgtttttca gcgcgaacat gagttatggc
     7261 gttcatgcat tgactaaaat tttagcagct gctgttcccc tacttgatga tttcgacatc
     7321 gaattgactg aggcacatca taataaaaaa gtagatgcac caagtggtac gttagaaaaa
     7381 ttgtatgatg tgatcgtatc tttgaaagaa aatgtaacac ctgtgtatga tagacatgaa
     7441 ttaaatgaaa aacgccagcc acaagatatt ggtatacatt ctattcgtgg aggtacgatt
     7501 gtcggtgaac atgaagttct atttgctggc actgatgaaa cgattcaaat cacgcatcgt
     7561 gcacaatcaa aagatatttt tgcgaatggt gcaatacaag cagcagaacg cttagttaat
     7621 aaaccaaacg gcttttatac gtttgacaac ttataaacat attaaaggag atcgattatt
     7681 ttatggtaca acatttaaca gctgaagaaa ttattcaata tataagtgat gctaaaaagt
     7741 ctacaccaat aaaagtatat ttaaatggta attttgaagg catcacatat ccagaaagtt
     7801 ttaaagtatt tggttcagaa caatctaaag taatcttttg tgaagcggat gattggaaac
     7861 ctttttacga agcatatggt agtcaattcg aagatataga aattgaaatg gatcgtcgca
     7921 attctgctat tccattaaaa gacttaacaa atacgaatgc acgaattgaa ccaggtgcgt
     7981 ttattagaga acaagccatt attgaagatg gtgctgtcgt tatgatgggc gcaacaatta
     8041 atattggcgc agtcgttggc gaaggtacaa tgattgatat gaatgctact ctcggtggtc
     8101 gtgctacaac tggtaaaaat gtacatgtag gggctggcgc agtattagca ggtgtgattg
     8161 aaccccctag tgcttcaccg gttataatcg aggatgatgt attaatcggt gcaaatgcag
     8221 ttattttaga aggtgtacgt gttggtaaag gtgctattgt tgcagctggc gcgattgtga
     8281 cacaagatgt accagctggt gcagttgttg ctggtacacc tgcaaaagtg attaagcaag
     8341 cttctgaagt acaagatact aaaaaagaga ttgtagcagc attaagaaaa ctgaatgact
     8401 agtgacgtca acgtataata atttcaaggt tgagatacga ttatgtctca accttattgt
     8461 ttagcattga ataaacttat gatcacaaat ttcgaaatac gttacactat ttatatagta
     8521 atttaacaca aaggattgat aattatgaat gaattagaat ttgttacgaa acatcgccgt
     8581 catttacatc aacatcctga attaagctta catgaatttg aaacaactgc ttatattaaa
     8641 gcgtttttag atagtttaaa tattaaatac gattgcccat tggaaactgg cgtcattgca
     8701 tacttagaag gtaatggctc acatacgata gcgtatagag ctgatattga tgcgttacct
     8761 attttagagg aaaatgatgt gccttatcgc agtcaatctg atcatgtgat gcatgcttgt
     8821 ggacatgatg gtcatacaac tgcattaatg ctttttgtac aacgttgcaa agacatgcaa
     8881 gatgcaggtc aattaccgca aaatgtcgtt ttcattttcc aacctgcaga agaaactggt
     8941 ggcggtgcaa atcgattaat aaaagccggt gcctttgata agtatccaat tgaagcggta
     9001 tttggtattc atgttaaccc atttgctgat gaaggcattg cagtgataag agatgaagaa
     9061 attacggcca gcgcaacaga gtatcgcttt ttcttaacag gcctgtcaag tcatgttgct
     9121 gataaagaac aaggtcattc ttgtggtgaa gcattacaac atgtattaac tcaaatatca
     9181 caaattcaac aatttcacct taacggtttg aaacgaaata ttgttcatat tggtcatttt
     9241 aaagctggtg aagcgattaa cactgtacca agtaatggct atttagaagg tactattcgt
     9301 acatatgata ttgatgattt aacaatcgtt aaaaatcaaa tgcacaagat agcagaaagt
     9361 gtcaagcttc tgtttaatgt agattgtgaa gttaaatttg cagaaggtta tccccctaca
     9421 atcaatagtc cgaaattacg tactcaaata gaggacgcct taataaaagc tgatttaaat
     9481 gtctatgaca aaccaacgcc attcttattt ggggaagatt ttagttttta tggtcaacaa
     9541 ctagctccag cttactttgt ttttatagga acacgaaatg aagataaagg ttttgtaact
     9601 ggtttgcaca catcacattt aaattttgat gaaaaagtgt taataaacgt ggttaatttt
     9661 tacgaaaatt tattaaataa ttacaaagag gtgtaataca ttgacagcaa catggtctgt
     9721 aaataagaaa atatttttac aaaatgcaat cacagtcaaa aacaatcagc cattaatggc
     9781 agttgttaaa aataatgcat atcactatga cctagaattt gctgtaactc agtttatcca
     9841 tgcaggtata gatacattta gcacaacatc actacgagaa gcaattcaaa ttagacaact
     9901 tgctccagat gcaacaatct ttttaatgaa tgcagtttac gagtttgatt tagtccgtga
     9961 acatcaaata catatgactt tgccgtcgtt gacatattac tataaccata aaaatgattt
    10021 agctggtatt catgttcact tagaatttga aaatttatta catcggtctg gatttaaaga
    10081 tttaaacgaa attaaagaag tattgaaaga tcaccatcat aatcaaaatg caaaaatgat
    10141 tattagtggt ttatggaccc attttggata tgctgatgaa ttcgatgtgt cagattataa
    10201 tgttgaacgt tcacaatgga tggaaattgt tgaagcactt ttatctgaag gttatcagtt
    10261 cgacctaatc catgctcaaa atagtgcgag tttttatcgg gaaggacaaa tattactacc
    10321 ccaccataca catgcgcgtg taggtattgc gttatacggt tcaagaccat atagttcact
    10381 gaatcaacat gatatagttc agtcattaac tttaaaagca catgttattc aagtgcgcga
    10441 agtacaagct ggtgattatt gcggttatag ctttgccttt gaagtgacta aaaacaatac
    10501 aaaattagct gtagttgata tcggttatgg cgatggaatt ttaagaactc gtgctaaaca
    10561 tgaagcactt atcaatggta aacgctaccc gatacgtgca ttaatgatga gccatatgtt
    10621 tgttgaagta gatggcaatg tacatgcaca agatgaagtt attctttata ataatgatat
    10681 ccgcatcgat gaatatacct ttaaaggtgt tggtgcaaat tctgaacaat taagtgctat
    10741 gaatcatgat tctttaaaaa aggagtacat ttcaaatgac tgttaaatat aatcaaaatg
    10801 gcgaattaac aatggatggt attagtttaa aaacgattgc acaaagcttt ggtacaccta
    10861 ccattgttta tgatgaacta caaattagag aacagatgcg ccgttaccat cgcgcattta
    10921 aagatagtgg attaaaatac aatatttcat acgcctcaaa ggcatttact tgcattcaaa
    10981 tggtcaaact tgtagctgag gaagatttac agttagatgt tgtttctgaa ggtgaattat
    11041 atacagcttt agaagcaggt tttgaaccga gtcgcatcca tttccatggt aacaataaaa
    11101 cgaaacatga aattaggtat gctttagaaa ataatatcgg ttattttgtt atagattcat
    11161 tagaagaaat tgaattaata gaccgctatg ctaatgatac ggttcaagtt gtattacgag
    11221 ttaatccagg tgttgaagca catacacacg aatttattca aactgggcaa gaagatagta
    11281 agtttggatt atcaattcaa tatggcttag ctaaaaaagc aattgacaaa gtccaacaat
    11341 ctaaacactt aaaattaaaa ggtgtacatt gtcatattgg ttcacagatt gaaggtacag
    11401 aagcatttat tgaaactgct aaaattgttt tacgttggct taaagagcaa ggcattcaag
    11461 ttgaattatt aaaccttggt ggtggctttg gtattaaata tgttgaaggt gacgaaagtt
    11521 tccctatcga aagtggtatt aaagatatta cagacgcaat aaaatccgaa attaaagttc
    11581 taggtataga tgcaccagaa ataggtattg aaccgggacg atcaattgta ggtgaagctg
    11641 gcgttacttt atatgaagtt ggaaccatta aagaaattcc agagattaat aaatatgttt
    11701 caatcgatgg cggtatgagt gatcatatca gaactgcact ttatgacgca aagtatcaag
    11761 cattgcttgt taatagaaat gaagaagcag atgacagtgt aactatagct ggaaaattat
    11821 gtgagtctgg tgatatcatt attaaagacg ctaaattacc ttcatcagtc aaacgtggag
    11881 actatcttgc tatattatca actggtgcat atcattactc tatggcatcc aattacaatc
    11941 aaatgcaaaa gccttctgtg tttttcttaa aagatggcaa agcacgtgaa gttataaagc
    12001 gacaatcgtt aagacaactc attattaatg atacaaaata aaaataaaca aagtaatccc
    12061 cgaagcacag aaaattatac tgtgactcgg ggaattttta tgcattatag atgactgtag
    12121 aaaacatgct tggacatata ctctaaaaaa ggaagaacag cacattttta gttagaaagt
    12181 atttatttct ttttgtcatt aatcctttat att
//
LOCUS       DAKTIO010000079        12060 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.079, whole genome shotgun sequence.
ACCESSION   DAKTIO010000079 DAKTIO010000000
VERSION     DAKTIO010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12060
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.079"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            74..1228
                     /locus_tag="O0293_001568"
     CDS             74..1228
                     /locus_tag="O0293_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCY0280454.1"
                     /translation="MHWTIIGGGIQGTAIAQKLLSSGLTTDRLTIIDPHETFCQRFNS
                     YTNRIEMPYLRSPIVHHVHPQPFHLKQFAKQHQYTNAFYGPYQRPELTMFMDHIAHAS
                     KQYQLEDCLVQGLVQTLDKQEDKWHIKLEDGQIITTDCVVIAIGSTNIPFMPDILKDK
                     QNVNHIFEKELDQVVYDKTDHIVGSGITAAHLALKLLNHDNDKKIHLWLNKDIEIHDF
                     DADPGWLGPKNMSSFLSTKSMPERNAIVQRERHKGSMPHELYLRLKKHIKNGRINVHK
                     TPITQISGGVINTENDSVPYQQIMVATGFEQDFMSQPLIKQLIQNYDAPINECNYPVI
                     SEKLEWIPNLFVAGCFADLELGPFGRNVMGGRKAAERIEQAFLKLQQYSA"
     gene            complement(1284..1658)
                     /locus_tag="O0293_001569"
     CDS             complement(1284..1658)
                     /locus_tag="O0293_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HCY0280455.1"
                     /translation="MTHLLETFEMSIDHQEDGLVVISMPVTDKVKQPFGYLHGGASIA
                     LGETACSLGSANLIDTTKFIPLGLEMNANHIHSAKDGRVTATAEIIHRGKSTHVWDIK
                     IKNDKEQLITVMRGTVAIKPLK"
     gene            complement(1677..2993)
                     /locus_tag="O0293_001570"
     CDS             complement(1677..2993)
                     /locus_tag="O0293_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HCY0280456.1"
                     /translation="MINAVVIAVILMIVLCLCRLNVVISLFISALVGGLISGMSIEKV
                     INVFGKNIVDGAEVALSYALLGGFAALISYSGITDYLVGKIINAIHAENSRWSRVKVK
                     VTIIIALLAMSIMSQNLIPVHIAFIPIVIPPLLSLFNDLKIDRRLIGLIIGFGLCFPY
                     VLLPYGFGQIFQQIIQSGFAKANHPIEFNMIWKAMLIPSMGYIVGLLIGLYVYRKPRE
                     YETRKISDSDNVTELKPYILIVTIVAILATFLVQTFTDSMIFGALAGVLVFFISRAYN
                     WYELDAKFVEGIKIMAYIGVVILTANGFAGVMNATGDIDELVKTLTSITGDNKLFSII
                     MMYVIGLIVTLGIGSSFATIPIIASLFIPFGASIGLDTMALIALIGTASALGDSGSPA
                     SDSTLGPTAGLNVDGQHDHIRDTCVPNFLFYNIPLMIFGTIAAMVL"
     gene            complement(3404..4879)
                     /locus_tag="O0293_001571"
     CDS             complement(3404..4879)
                     /locus_tag="O0293_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HCY0280457.1"
                     /translation="MNFKLNNTLSNEINTLIIGIPEHLNQLERISFNHIDITESLERL
                     KHQHIIGSKVGKIYTTAFDVQDQTYRLITVGLGNLKTRSYQDMLKIWGHLFQYIKSEH
                     IEDTYLLMDSFISKYDQLSDVLMACGIQSERATYEFDHYKSSKKAPFKTNLNLISESL
                     IELDFIHEGISIGQSINLARDFSNMPPNVLTPQTFAEDIVNHFKNTKVKVDVKDYDTL
                     VSEGFGLLQAVGKGSKHKPRLVTITYNGKDKDEAPIALVGKGITYDSGGYSIKTKNGM
                     ATMKFDMCGAANVVGIIEAASRLQLPVNIVGVLACAENMINEASMKPDDVFTALSGET
                     VEVMNTDAEGRLVLADAVFYANQYQPSVIMDFATLTGAAIVALGDDKAAAFESNSKVI
                     LNDILQISSEVDEMVFELPITATERASIKHSDIADLVNHTNGQGKALFAASFVTHFSG
                     QTPHIHFDIAGPATTNKASYNGPKGPTGFMIPTIVQWLKQQ"
     gene            complement(5010..6218)
                     /locus_tag="O0293_001572"
     CDS             complement(5010..6218)
                     /locus_tag="O0293_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0280458.1"
                     /translation="MAQDRKKVLVLGAGYAGLQTVTKLQKAISTEEAEITLINKNEYH
                     YEATWLHEASAGTLNYEDVLYPVESVLKKDKVNFVQAEVTKIDRDAKKVETNQGIYDF
                     DILVVALGFVSETFGIEGMKDHAFQIENVITARELSRHIEDKFANYAASKEKDDNDLS
                     ILVGGAGFTGVEFLGELTDRIPELCSKYGVDQNKVKITCVEAAPKMLPMFSEELVNHA
                     VSYLEDRGVEFKIATPIVACNEKGFVVEVDGEKQQLNAGTSVWAAGVRGSKLMEESFE
                     GVKRGRIVTKQDLTINGYDNIFVIGDCSAFIPAGEERPLPTTAQIAMQQGESVAKNIK
                     RILNGESTEEFEYVDRGTVCSLGSHDGVGMVFGKPIAGKKAAFMKKVIDTRAVFKIGG
                     IGLAFKKGKF"
     gene            complement(6672..7031)
                     /locus_tag="O0293_001573"
     CDS             complement(6672..7031)
                     /locus_tag="O0293_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HCY0280459.1"
                     /translation="MPTVILTEAAAYEVKDMLKANEMPDGYLKIKVNGGGCTGLTYGM
                     GAEEAPGENDEVLEYFGLKVLVDKKDAPVLNGTTIDFKQSLMGGGFQIDNPNAIASCG
                     CGSSFRTAKVAGNPENC"
     gene            complement(7044..7280)
                     /locus_tag="O0293_001574"
     CDS             complement(7044..7280)
                     /locus_tag="O0293_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzB family protein"
                     /protein_id="HCY0280460.1"
                     /translation="MNPIVEFCLSNMAKGGDYVFNQLENDPDVDVLEYGCLTHCGICS
                     AGLYALVNGDIVEGDSPEELLQNIYAHIKETWIF"
     gene            7597..8661
                     /locus_tag="O0293_001575"
     CDS             7597..8661
                     /locus_tag="O0293_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0280461.1"
                     /translation="MKNLVLLGGGYGNMRIMSRILTTSLPQDYTVTLVDRMPFHGLKP
                     EFYALAAGTKSDKDVRMKFPNHPQVNTVYGEINDIDLDAQIVSVGNSKIDYDELIIGL
                     GCEDKYHNVPGAEEYTHSIQTLSKARDTFHSISELPEGAKVGIVGAGLSGIELASELR
                     ESRSDLEIYLYDRGPRILRNFPEKLSKYVAKWFAKNNVTVVPNSNINKVEPGKIYNCD
                     EPKDIDLVVWTAGIQPVEVVRNLPIDINSNGRVIVNQYHQVPTYRNVYVVGDCADLPH
                     APSAQLAEVQGDQIADVLKKQWLNEPLPDKMPELKVQGIVGSLGDKQGFAYIMDRTVT
                     GRLASILKSGVLWLYKYHNG"
     gene            complement(8720..9043)
                     /locus_tag="O0293_001576"
     CDS             complement(8720..9043)
                     /locus_tag="O0293_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzD family protein"
                     /protein_id="HCY0280462.1"
                     /translation="MEHVSVVVYGADVICASCVNAPTSKDIYDWLQPLLKRKYPNISF
                     KYTYIDITKDNDNLTDHDLQFIERIEQDELFYPLITMNDEYVADGYIQTKQITRFIDQ
                     KLVNE"
     gene            9144..9386
                     /locus_tag="O0293_001577"
     CDS             9144..9386
                     /locus_tag="O0293_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HCY0280463.1"
                     /translation="MPTEDTTMFDQVAEVIERLRPFLLRDGGDCSLIDVEDGIVKLQL
                     HGACGTCPSSTITLKAGIERALHEEVPGVIEVEQVF"
     gene            complement(9649..10824)
                     /gene="dltD"
                     /locus_tag="O0293_001578"
     CDS             complement(9649..10824)
                     /gene="dltD"
                     /locus_tag="O0293_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HCY0280464.1"
                     /translation="MKLKPFLPILISGAVFIVFLLLPASWFTGLVNEKTVEDNRTSLT
                     DQVLKGTLIQDKLYESNKYYPIYGSSELGKDDPFNPAIALNKHNANKKAFLLGAGGST
                     DLINAVELASQYDKLKGKKLTFIISPQWFTNHGLTNQNFDARMSQTQINQMFQQKNMS
                     TELKRRYAQRLLQFPHVHNKEYLKSYAKNPKETKDSYISGFKENQLIKIEAIKSLFAM
                     DKSPLEHVKPATKPDASWDEMKQKAVEIGKADTTSNKFGIRDQYWKLIQESKRKVRRD
                     YEFNVNSPEFQDLELLVKTMRAAGADVQYVSIPSNGVWYDHIGIDKERRQAVYKKIHS
                     TVVDNGGKIYDMTDKEYEKYVISDAVHIGWKGWVYMDEQIAKHMKGEPQPEVDKPKN"
     gene            complement(10821..11057)
                     /gene="dltC"
                     /locus_tag="O0293_001579"
     CDS             complement(10821..11057)
                     /gene="dltC"
                     /locus_tag="O0293_001579"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     2"
                     /protein_id="HCY0280465.1"
                     /translation="MEFREQVLNLLAEVAENDIVKENPDVEIFEEGIIDSFQTVGLLL
                     EIQNKLDIEVSIMDFDRDEWATPNKIVEALEELR"
     gene            complement(11075..>12060)
                     /gene="dltB"
                     /locus_tag="O0293_001580"
     CDS             complement(11075..>12060)
                     /gene="dltB"
                     /locus_tag="O0293_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PG:teichoic acid D-alanyltransferase DltB"
                     /protein_id="HCY0280466.1"
                     /translation="MFYYHSKPKNNSFSKFVTVMVLSILPLALVKVLQSTWLGGHQIH
                     FHESKLIEFVGFLGISYVTFKSVQLIMEIRDGSIKEIKVWKLIQFISFFPTISSGPID
                     RYKRFVKDDKKVPTGNEYRELVLKAIHMIMLGFLYKYIVAYFINTYAIMPLQLDLHGF
                     VNLWLYMYAYSLYLFFDFAGYSLFAIAFSYLFGIKTPPNFDKPFKAKNIKDFWNRWHM
                     TLSFWFRDCIYMRSLFYMSRKKLLKSQFAMSNVAFLINFFIMGIWHGIEVYYIVYGLY
                     HAALFIGYGYYERWRKKHPPRWQNGFTTALSIVITFHFVTFGFLIFSGKLI"
BASE COUNT         3971 a         2169 c         1770 g         4150 t
ORIGIN      
        1 atgtcgttat gtattatgat aataataatt ataaatcgta acgattacgt tttaaaaaga
       61 gagaggtttt attatgcatt ggacaattat cggcggtggc atacagggaa ctgcaatcgc
      121 acaaaaacta ttatcaagcg gattaacaac agaccgatta acaatcattg acccacacga
      181 aactttttgc caaaggttta actcatatac aaatcgaata gaaatgcctt atttaagatc
      241 accgattgta catcacgtac atccacaacc gttccatcta aaacaattcg ctaaacagca
      301 ccaatataca aatgcttttt atggtcctta tcaacgacct gaattgacaa tgtttatgga
      361 tcatattgca catgcttcta aacaatatca attagaggat tgcttggttc aaggtttagt
      421 tcaaacttta gataaacaag aagacaaatg gcatatcaag ttagaagatg gacaaattat
      481 cactacagat tgcgtcgtta ttgcaatagg cagtacaaat attccgttta tgcctgacat
      541 tttaaaagac aaacagaatg taaatcatat cttcgagaaa gaacttgatc aagtagtata
      601 tgataagacc gatcatatcg ttggtagcgg cattactgct gcacatcttg cacttaaatt
      661 gttaaatcat gataacgata aaaagattca tttatggcta aataaagata ttgaaataca
      721 tgactttgat gctgatcctg gttggttagg tccgaaaaat atgtcttcat ttttaagtac
      781 taaaagcatg cctgaaagaa atgccattgt acaacgcgaa cgtcataaag gatcaatgcc
      841 tcacgaactg tacttacgcc ttaaaaaaca tattaaaaat ggtcgtataa atgtgcataa
      901 aacacctatc actcaaatta gtggtggtgt aattaacact gaaaatgatt ctgttccata
      961 tcaacagatt atggttgcaa ctggttttga acaagatttt atgtcacaac cacttattaa
     1021 gcaattaata caaaattatg atgcacctat caacgaatgt aattaccctg ttatttccga
     1081 aaaattagaa tggataccaa atctatttgt cgcaggatgc tttgcagact tagaattagg
     1141 accatttggt agaaatgtta tgggtggccg taaagctgcc gaacgcattg aacaagcatt
     1201 tctaaaacta caacaatata gcgcataaaa aaactgctag ccgtagttat gaatatctca
     1261 taacatttca gctagcagtt cttttatttt aaaggtttaa tagcaactgt accacgcata
     1321 actgtaatta attgttcttt gtcattctta atttttatat cccatacatg tgtcgactta
     1381 cctcgatgaa taatttcagc tgtcgcagta acacgaccat ctttagcaga atgaatatgg
     1441 ttagcattca tctctaaacc taatggaata aatttggttg tatcaattaa attagcagat
     1501 cctaatgaac atgctgtttc acctaaagca atcgaagccc caccatgtaa atatccaaat
     1561 ggttgtttta ctttatcagt aacaggcata gaaataacaa ctaaaccatc ttcctggtga
     1621 tctattgaca tctcaaatgt ctctaataaa tgagtcatat tttcacctct ttatgcttat
     1681 agtaccatag cagcaatagt accgaaaatc attaaaggaa tattataaaa caagaagttt
     1741 ggtacacatg tatcacgtat atgatcatgt tggccatcaa catttaatcc cgcagttggt
     1801 cctaatgttg aatcacttgc aggcgaacct gagtcaccta atgcactcgc tgttccaatc
     1861 aatgcgatta atgccattgt atctagtcca attgacgctc caaaaggaat gaataatgat
     1921 gcgataatag gaattgttgc aaatgatgat ccaataccta aagtgacaat taaacctatc
     1981 acatacatca tgataatgct aaataattta ttatcaccag taatacttgt taaagtttta
     2041 actaattcat ctatatcacc agtagcattc attacaccag caaatccatt tgctgttaaa
     2101 ataactacac caatataagc cataatttta ataccttcaa caaacttagc atctaattca
     2161 taccaattat atgcacgtga aataaagaat acgagtaccc ctgccagtgc accaaaaatc
     2221 attgaatctg taaatgtttg tactaaaaat gtagctagta ttgctacaat tgttactatt
     2281 aagatatatg gttttaactc tgtaacattg tcactatctg aaattttacg tgtttcatat
     2341 tcacgtggtt tacgatatac atataaaccg ataagtaagc caacaatata ccccattgaa
     2401 ggaataagca ttgctttcca aatcatatta aactcaattg ggtgatttgc ctttgcaaag
     2461 ccactttgaa taatttgctg gaaaatttga ccgaatccat atggtaataa cacatacggg
     2521 aaacataaac caaaaccgat aatcaaaccg attaaacgtc tatctatttt taagtcatta
     2581 aacagactta acaatggtgg gatgacaatt ggaatgaatg caatatgtac aggaattaag
     2641 ttttgactca tgatactcat agctaataat gcaatgatta ttgtcacttt gactttaact
     2701 cttgaccatc gactattttc agcgtgaatt gcatttataa tttttcctac taaatagtct
     2761 gtgataccac tgtatgaaat taatgctgca aatccaccta ataaagcata gcttaatgct
     2821 acctcagcac catcgactat atttttccca aatacattta taactttttc aatgctcatg
     2881 cctgaaatta agccaccaac tagcgcactg ataaataagc ttataactac gtttaatcga
     2941 cataaacata gcacaatcat taaaattact gctattacta ctgcatttat catatgacaa
     3001 ccatccttta tcactctatc gaactaaagc gtttatagac tacatagtat cagtagcttt
     3061 aacacctgtc aatagcaaac tataccaata atctttattt tttagttaat tgtctttctt
     3121 aaaataattt tagctttcat taaattaaac aattttacaa gcttggaaca ccaatcaaaa
     3181 tcctaagttc taaaatgcaa tattagtagt cgttgactga atgaacatat gcttataata
     3241 tttttttgca atgctagtca agttgattta tgctcacaag gatatgcgat ttatattttc
     3301 ttacaacaat gaaaatgcct gatacaatgc gatcctttat actttcaaga gttaaattag
     3361 aaatgtatat caagacacaa gttgtatcag gcaaataatt aaattattgt tgttttaacc
     3421 attgtactat cgtcggaatc ataaatcctg ttggcccttt tggaccatta tatgaagctt
     3481 tattagtcgt tgctggacct gcaatatcga aatgaatgtg aggtgtttga ccactaaaat
     3541 gtgttacaaa acttgccgca aatagcgctt taccttgtcc attcgtatgg ttaactaaat
     3601 cagcgatatc actgtgttta atacttgcac gttcggttgc agtaatcggt aattcaaata
     3661 ccatttcatc gacttcagaa cttatttgta atatatcgtt taatatcact ttactattcg
     3721 attcaaatgc agcagcttta tcatcgccta gtgcaacaat tgctgcaccc gttaatgtag
     3781 caaagtccat aatcacacta ggctgatatt gatttgcata aaacacagca tctgcaagga
     3841 ctaatctacc ttcagcgtct gtattcatta cttctacagt ttcaccactt aatgctgtaa
     3901 atacatcatc tggcttcatt gatgcttcat ttatcatatt ttcagcacac gcaagcactc
     3961 cgacaatatt tacaggcagt tgtaaacgac tagccgcttc aatgatacca acgacattcg
     4021 cagcgccaca catgtcaaac ttcattgtag ccatgccatt cttcgtttta atactataac
     4081 caccagaatc atacgttata cctttaccaa ctaaggcaat tggtgcttca tctttgtctt
     4141 tgccattata tgtgatggtt actaatctcg gtttatgctt actaccttta cctactgctt
     4201 gtaaaagtcc gaatccttca gaaactaaag tgtcataatc tttaacatct actttgacct
     4261 ttgtattttt aaaatgatta acaatatctt ctgcaaatgt ttgtggtgtt aatacattcg
     4321 gtggcatatt actaaagtct cttgccaaat taatggattg gccaatactg ataccctcat
     4381 gaataaaatc taattcaatt aatgattcac taataaggtt taaattcgtc ttaaacggtg
     4441 ccttcttact tgatttataa tgatcgaatt catatgttgc acgctcactt tgaataccgc
     4501 atgccattaa tacatctgat aactgatcat attttgaaat aaatgaatcc ataagtaaat
     4561 acgtatcttc aatgtgttct gactttatgt attggaaaag atgtccccat attttcaaca
     4621 tatcttgata actacgtgtc tttaagtttc ctaaaccaac tgtaattaaa cgatatgttt
     4681 gatcttgtac atcaaatgca gttgtataaa tcttcccaac tttactacca ataatatgtt
     4741 gatgttttag tctttcaagt gattctgtaa tatcgatatg attaaaacta atgcgctcta
     4801 actgatttaa atgttctggt ataccaataa tcaatgtatt tatttcgttg cttagtgtgt
     4861 tatttaattt aaaattcata atgtacctcc ttagatttga tatgttactc acttcaaatt
     4921 gtacaacaat aaagcccttc agtgacactg aaggacttta taaatgaata atttaatttt
     4981 atgtgtttag ctgaaacttt atttaaagcc tagaatttac cttttttgaa tgctaaaccg
     5041 ataccaccga ttttgaatac cgcacgtgta tcaatcactt ttttcatgaa tgctgctttt
     5101 ttaccagcga taggtttacc aaataccata cctacaccgt catgtgaacc taaagaacaa
     5161 acagttccac gatcaacgta ttcgaattct tcagttgatt caccgtttaa gatgcgttta
     5221 atgtttttag caacactttc accttgttgc attgcaattt gtgctgtagt tggtaatgga
     5281 cgttcttctc cagctgggat aaacgctgaa cagtcaccaa taacaaaaat gttgtcgtaa
     5341 ccattgattg ttaaatcttg ctttgtaacg atacgtccac gtttaacgcc ttcaaatgat
     5401 tcttccatta atttactacc acgtacacca gctgcccata ctgaagtacc tgcatttaat
     5461 tgttgttttt caccatctac ttcaactaca aaaccttttt cgttacaagc aacgattggt
     5521 gtagcaattt taaattcaac accgcggtct tctaagtagc taactgcgtg gttaactaat
     5581 tcttctgaga acattggtaa cattttaggt gctgcttcaa cacaagtgat tttaacttta
     5641 ttttgatcca caccatattt gctacataat tcaggaattc tgtctgttaa ttcacctaag
     5701 aattcaacac cagtgaatcc agcaccacca actaagatag ataaatcgtt atcatctttt
     5761 tcttttgatg ctgcatagtt agcaaatttg tcttcgatat gacgtgataa ttcacgtgct
     5821 gtgataacat tttcaatttg gaaagcatga tctttcatac cttcgatgcc gaatgtttca
     5881 ctaacgaaac ctaatgctac tactaaaata tcaaagtcat aaataccttg atttgtttct
     5941 acctttttag catcacggtc aatttttgtt acttctgctt gaacaaagtt cactttgtct
     6001 ttcttcaaga cactttccac aggatataat acatcttcat agtttagtgt acctgctgat
     6061 gcttcatgta accatgttgc ttcatagtga tattcatttt tattaataag cgtaatttct
     6121 gcttcttctg ttgatatcgc tttttgcaat ttagttacag tttgtaaacc tgcataacca
     6181 gcaccaagta caagtacttt tttacgatct tgagccattt aatttcacct aagctttcat
     6241 atttttttaa ccaaatgctg ataattacta gtaacaactg gtcattacta cttttaccac
     6301 aatgtatact agtttatcat cattttgtta tttaagtctt ttaaattatg taaaaacctg
     6361 tgatgttcac aaaaaagaca tttttaatcc aatactaatt ctatagcttt tatgttatat
     6421 tttcaagcaa gattcagtca gattgtgaaa aaattaatta attttttcgt agcctgattt
     6481 cgattaaaaa tgtatccgct atcacgatca tttaaaatac tatttatagt cagattctta
     6541 attttcaata agctttttaa taaaacaaaa tatctttacg caaaaatagg acataatcaa
     6601 atatgtcctt aatcgttatt gatatcaatt tgtatcccaa aaattgggtt aaccattaaa
     6661 aagttatttt attagcaatt ttcaggatta cctgcaactt ttgcagttct aaatgaacta
     6721 ccacagccac atgaagcaat tgcattagga ttgtcgattt ggaaaccgcc acccattaat
     6781 gattgcttaa aatcaatagt cgtaccattt aatacgggtg catctttttt gtctactaat
     6841 acttttaatc caaagtattc taagacttca tcattttcac caggcgcttc ttctgcaccc
     6901 ataccgtatg ttaaaccagt gcacccgcca ccattcactt taatttttaa atagccatct
     6961 ggcatttcat ttgctttaag catatctttt acttcgtaag cagctgcttc tgttaatata
     7021 actgttggca tgataactcc tccttaaaaa atccaagttt cttttatatg tgcatatata
     7081 ttttgtaata attcttccgg cgaatcacct tcaacaatat caccatttac taaagcatac
     7141 aacccggctg aacatatacc acaatgtgtc aggcaaccat actctaacac atcgacatct
     7201 gggtcatttt ccagttgatt aaaaacataa tctccacctt ttgccatgtt agagagacaa
     7261 aattctacga tcggattcat acttcacctt cttatttcat ttgttacaat attatagcat
     7321 tttaaaactg gtattttaac atgatgtgct caattagcaa caactgatgt ttcttatccc
     7381 agttatgtaa tagtgcctta gttagtacaa atatttattt gtagtcattt tcaaaaaaat
     7441 atataataag catataaata catatttact tttttagggg ttattgatga atcaaagaca
     7501 cttgcatttt ataactgttc attaacaaat atatctatgc actttgatct tttcaaaatt
     7561 aaagtaaata ttaacatata aaaggggtac ttcattatga aaaacttagt tttgttaggc
     7621 ggcggatatg gtaatatgcg tatcatgtca cgcattttaa ctacttcttt accacaagat
     7681 tatacagtca cattagttga tcgtatgcca tttcatggat tgaaaccaga attttatgct
     7741 ttagctgcgg gcacgaaatc agataaagat gttcgtatga aattccctaa tcatccacaa
     7801 gtgaatacag tttatggtga aattaacgac atagatttag atgctcaaat tgtctcagtc
     7861 ggtaattcta aaattgatta tgatgagcta atcattggtt taggatgtga agataaatat
     7921 cataacgttc caggagccga agaatataca catagtattc aaacactctc aaaggctcgg
     7981 gatactttcc atagtattag tgaactacca gaaggtgcta aagtcggtat cgttggtgct
     8041 ggattaagcg gcatagaact tgccagcgaa ttaagagaaa gtagatcaga cttggaaata
     8101 tatctttatg accgtgggcc gcgaatttta agaaattttc cagaaaaatt aagtaagtat
     8161 gttgcgaaat ggttcgccaa aaataatgtt accgttgttc caaattcaaa tattaataaa
     8221 gttgaacctg gtaaaatata taactgtgat gaacctaaag atattgattt agttgtatgg
     8281 acagcaggaa ttcaacctgt tgaagttgtt cgtaacttgc cgattgatat aaatagtaat
     8341 ggacgcgtga tagttaacca gtatcatcaa gtaccaacat atcgtaacgt ctatgtagtt
     8401 ggtgattgtg ctgatttacc acatgcgcca agtgctcagt tagccgaagt tcaaggtgat
     8461 caaattgccg atgtgcttaa aaagcaatgg ctaaatgaac cattacctga caaaatgccg
     8521 gaactaaagg tacaaggtat cgttgggtca ttaggagata agcaaggttt tgcatatatc
     8581 atggaccgca ctgtaacagg acgactcgct tctattttaa aatccggcgt actttggtta
     8641 tataaatatc acaatggtta aaattaaaaa gcaatgtcag ttcacactat tactgacatt
     8701 gcttttagtt atgtgtatat tattcattca caagcttttg gtctataaat cgagtgattt
     8761 gctttgtttg tatataacca tctgctacat attcatcgtt cattgtaatt aatggataaa
     8821 atagttcatc ttgttctatt ctttcaataa attgtaaatc atgatctgtt aagttgtcat
     8881 tatcttttgt aatatctata tacgtatatt taaatgatat atttgggtat tttcttttta
     8941 atagcggctg tagccagtca taaatatctt tcgatgttgg cgcattaacg caacttgcac
     9001 atataacatc tgccccataa actaccacac tcacgtgctc catatattta tcccccattg
     9061 gtttgataga tttttattac actatctatt ataatatact aataaagatt atcaaattca
     9121 tctctcgaaa ggagacttgc ctgatgccta ctgaagatac aacgatgttt gatcaagtag
     9181 cagaagttat tgaacgtctt cgtccatttt tattacgtga tggtggcgac tgctcattga
     9241 ttgacgtgga agacggtatt gttaaattac aattacatgg tgcatgtggt acatgcccaa
     9301 gttctacaat cactcttaaa gctggtattg agcgtgcatt acacgaagaa gtgcctggtg
     9361 taatagaagt agaacaagta ttctaatata aagatattaa gttaacagaa catattatga
     9421 ataacaacaa agacaggaat actgaattat ttataatcag ttattacctg tcttttttcg
     9481 ctgtcaaatt ggactgatgg gaaagttatt ttatactatt tatctgatta attattttat
     9541 tattgccaca tatccaagca catattacta ttctattcta tgaaatttaa aatataaaaa
     9601 tagcacatac gctcaatcaa aatcgttgag ttatgtgcta tttgtatttt aatttttagg
     9661 tttatctact tcaggttgtg gttcaccttt catatgtttc gcaatttgct catccatata
     9721 aacccaacct ttccaaccga tgtgtacggc atcactgata acatattttt catattcttt
     9781 atcagtcata tcgtaaattt taccaccatt atctacaaca gtagaatgga tttttttata
     9841 aactgcttga cgacgttctt tatcgatacc aatgtggtca taccatacac cgtttgatgg
     9901 aatacttaca tattgaacat ctgcaccagc agcacgcatt gtttttacaa gtaattctaa
     9961 atcttggaat tctggagaat taacattgaa ttcgtagtca cgtctaactt tacgcttact
    10021 ttcttgaatt aatttccagt attgatctct aataccaaat ttattcgatg tagtatcagc
    10081 tttaccaatt tcaactgctt tttgtttcat ctcatcccaa gaagcgtctg gttttgtagc
    10141 aggtttaaca tgttctaatg gagatttatc cattgcaaac aatgatttaa tcgcttctat
    10201 tttaatcaat tgattctctt taaaaccaga aatataacta tctttagttt ctttagggtt
    10261 tttagcataa gatttcaagt attctttatt gtgtacatgt ggaaactgta ataaacgttg
    10321 tgcataacga cgttttaatt cagtagacat gtttttctgc tggaacattt gattaatttg
    10381 agtttgagac atacgagcat caaagttttg attcgttaaa ccatggtttg taaaccattg
    10441 tggtgaaata ataaatgtta atttcttacc ttttaattta tcatactgtg atgcaagttc
    10501 aactgcgtta attaagtctg tagaaccacc agcacctaat aagaatgctt ttttgttggc
    10561 gttatgctta tttaatgcaa ttgcaggatt aaatgggtca tctttaccta attcactaga
    10621 gccgtatata ggataatact tgtttgattc gtataactta tcttgaatga gtgtgccttt
    10681 tagtacttga tctgtcaatg aagttctatt atcttctaca gtcttttcat ttactaatcc
    10741 tgtaaaccaa ctagcaggta ataatagaaa gacaatgaat accgctccac taattaaaat
    10801 gggtaaaaaa ggttttaatt tcatcgtaac tcttctaatg cttcaacgat tttatttggt
    10861 gttgcccact catctctatc aaagtccata atagatactt cgatatcaag tttattttga
    10921 atctctaata ataatccaac tgtttggaaa gaatcaataa taccttcttc aaaaatttct
    10981 acgtctggat tttcttttac aatatcattt tctgctactt ctgctaataa atttaatact
    11041 tgttctctaa attccataat taaattctcc tttattatat aagtttacct gagaagatta
    11101 aaaagccaaa tgttacaaag tggaatgtaa tcacaatgct aagtgctgtt gtgaaaccat
    11161 tttgccaacg tggcggatgt ttcttacgcc aacgttcata atagccataa cctataaaca
    11221 atgctgcatg gtataaacca taaacaatgt aatacacttc gataccatgc caaattccca
    11281 ttatgaagaa gttgattaag aatgccacgt tagacattgc aaattgactc ttcaataatt
    11341 ttttacgaga catgtagaat aaagatctca tgtaaataca atctctgaac cagaatgata
    11401 atgtcatatg ccatctattc cagaaatctt taatattttt cgctttgaaa ggtttatcga
    11461 agtttggtgg tgttttaata ccgaataaat aactaaatgc tatcgcaaat aaactataac
    11521 ctgcaaagtc aaagaataaa tataagctgt atgcgtacat atataaccac aaattgacaa
    11581 agccatgtaa gtctaattgt aacggcatga ttgcatatgt gttaataaag taagcaacaa
    11641 tatatttata caagaaacca agcataatca tgtgaattgc ttttaatact aattcacgat
    11701 attcattgcc tgttggtact tttttatcgt ctttaacgaa acgtttgtaa cgatcgattg
    11761 gtccagatga aatcgttggg aagaatgaaa taaattgaat taatttccat actttaattt
    11821 ctttgataga accatcacga atttccataa ttaactgcac acttttgaat gtaacataag
    11881 aaattcctaa gaaaccaaca aattcaatta atttactttc atggaagtga atctgatgtc
    11941 cacctaacca tgtactttgt aacactttca caagtgctaa tggcaatatt gataaaacca
    12001 ttacagttac aaattttgaa aatgaattat tttttggttt tgaatgataa taaaacatta
//
LOCUS       DAKTIO010000080        12001 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.080, whole genome shotgun sequence.
ACCESSION   DAKTIO010000080 DAKTIO010000000
VERSION     DAKTIO010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12001
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.080"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            360..2315
                     /locus_tag="O0293_001581"
     CDS             360..2315
                     /locus_tag="O0293_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0280467.1"
                     /translation="MTDNYSLHIYKSMKMSSVKEANLKIIYWLQGTGVVSINLQQYDL
                     KRNYVTIIMLNDLYQIDGNDDSVCCVVEVSAKTFLKFMNANYMMRGKILTNEVASTFR
                     ILIKYLIYSKIKQQSYNANDKIINYMCVELMSLNTYDGSRHLVAEEVHDYLTNNHHKK
                     INRKDVINQVSITNKALSDMFKATPYSNFIQYLNHIRLEHCLIDILTSKKPIEEIASS
                     HGFNHYSRFIHLFKETYGNTPKLIRKRYSPTSHSINHSQLVEIDKNIIELFDDTNQSS
                     SSMREIDIPFEPTKRSQMYQPKNIYIKGSNFYWMDYYTIKQIIQRLGVNPENLHIIVT
                     IDTSNELSIEQITLLLQKIVNYNVNVVFRIKHEYKELLTSAERGKIEKLVATIFNMTF
                     ESNRCKIAFLINDLNTTAIQKLKRLIDNYIGVFELIYHLEPKELNTRYDKEIDHLIDY
                     FILPLRQIEQVSIARSKIICDVDLINENNDLKEKITMSDINKVSELLTNYTKFRGLIM
                     HMSIISESSKEINLTRLSIFIFIINILNQLRGIVIYQNDTMIVTKFKHEVQCVICLPA
                     ALLNENSDAIRLSCKGLQQFSHAKTKQIVLSIDKDNHTSENGVLVNDIMQQHSYWKAN
                     FIERNIYDHSLMVSSNSIVHLKYHLEI"
     gene            2341..3084
                     /locus_tag="O0293_001582"
     CDS             2341..3084
                     /locus_tag="O0293_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HCY0280468.1"
                     /translation="MSKFKVKRLTAHILMLKVINDMLKYSFHLTLTQVKLLNLLVKYD
                     NSDSNSASIDSLLKMKEIQSKMALLQMLSYLNNHQWLLKGRDQRDQRKLTISINKMHI
                     DKIEYMNNELSDYIERYFGQYTFDFSCDYVSYLLSSQELLRNLKCYLNMCQLSLEELY
                     VLGILNLHKGQLTVKELQGEFHHPIFAVSPILKCLILKGLVKKERCELDERRVIVTIK
                     REKFSKVTMLIQACYNYLEKGIQVNLNNK"
     gene            3148..3474
                     /locus_tag="O0293_001583"
     CDS             3148..3474
                     /locus_tag="O0293_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCY0280469.1"
                     /translation="MILDNVNPNDLFPTEKKGPSVLGIIEYQVQGENEFEGAFIATNE
                     RLIMNVDMNGQFYYRSISYNEIEKIDYDGQTIMFKFNIGNVPMHDIKSANVEMFVEYV
                     KQHMIV"
     gene            3902..4249
                     /gene="sarR"
                     /locus_tag="O0293_001584"
     CDS             3902..4249
                     /gene="sarR"
                     /locus_tag="O0293_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarR"
                     /protein_id="HCY0280470.1"
                     /translation="MSKINDINDLVNATFQVKKFFRDTKKKFNLNYEEIYILNHILRS
                     ESNEISSKEIAKCSEFKPYYLTKALQKLKDLKLLSKKRSLQDERTVIVYVTDTQKANI
                     QKLISELEEYIKN"
     gene            complement(4438..5274)
                     /locus_tag="O0293_001585"
     CDS             complement(4438..5274)
                     /locus_tag="O0293_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HCY0280471.1"
                     /translation="MDEQQWTGQLDLTVFFDGNRSVSRDIFFEKALKVIRPVYLNQST
                     IPTFYIVNVGGGYLDGDRYRMNVNVEDNAKVTLTSQGATKIYKTPSNHVEQYQTFNLK
                     DNAYLEYVADPIIAYENAKFYQHNTFNLNNSSSLFYTDILTPGYSKTGEAFKYQYMHL
                     INEIYIEDELVTYDNLLLNPNKQSINEIGYMEHYSHYGSAYFIHEDVNQKLIDSVYET
                     ISSYSNTFDCRVAISQLPTHGFAVRIFAYRTQIIEKILGTIQSYIAENIYDRKLDFLR
                     KY"
     gene            complement(5274..5888)
                     /gene="ureG"
                     /locus_tag="O0293_001586"
     CDS             complement(5274..5888)
                     /gene="ureG"
                     /locus_tag="O0293_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HCY0280472.1"
                     /translation="MANPIKIGIGGPVGAGKTQLIEKVVKRLSKEMSIGVITNDIYTK
                     EDEKILVNSGVLPESRIIGVETGGCPHTAIREDASMNFAAIDELLERHDDIELIFIES
                     GGDNLAATFSPELVDFSIYIIDVAQGEKIPRKGGQGMIKSDFFVINKTDLAPYVGASL
                     EQMAEDTKVFRGKRPFTFTNLKTDEGLDEVIDWIERDTLLKGLS"
     gene            complement(5901..6590)
                     /locus_tag="O0293_001587"
     CDS             complement(5901..6590)
                     /locus_tag="O0293_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HCY0280473.1"
                     /translation="MIDHTHLRLFQFCDSQFPTGAFSHSFGLETYIQRNIIHDDHTFI
                     AWLKMFLQEQLTYSDGLAMRLVYDALENDDTQKVLHIDKLMFVQNLPKETRVGAKQMG
                     TRMVKLALELYNSPWIAWYHQQMQDKKAKLNPAICFTMLGHHLGVDIETIIDYYLYQN
                     VSSLTQNAVRAIPLGQTAGQKIVTHMIPYIEGTRKQIFELKEADFGMTAPGLELNQMA
                     HENVNVRIFIS"
     gene            complement(6583..7035)
                     /gene="ureE"
                     /locus_tag="O0293_001588"
     CDS             complement(6583..7035)
                     /gene="ureE"
                     /locus_tag="O0293_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HCY0280474.1"
                     /translation="MIVEEIQGNIANLSNSEKQKHVEKVYLENSDLVKRIQRVVTDHG
                     TEIGIRLKQPIDLQYGDILYADDHNMIIVDVNSEDLLVIQPRTLQEMGGIAHQLGNRH
                     LPAQFTETEMLVQYDYLVEDLLKSLGIPYVREDRKVNKAFRHIGHSHD"
     gene            complement(7048..8763)
                     /gene="ureC"
                     /locus_tag="O0293_001589"
     CDS             complement(7048..8763)
                     /gene="ureC"
                     /locus_tag="O0293_001589"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HCY0280475.1"
                     /translation="MSFKMTQNQYTSLYGPTVGDSIRLGDTNLFAQIEKDYAVYGEEA
                     TFGGGKSIRDGMAQNPRVTRDDVNVADLVISNAVIIDYDKVVKADIGIKNGYIFAIGN
                     AGNPDIMDNVDIIIGSTTDIIAAEGKIVTAGGIDTHVHFINPEQAEVALESGITTHIG
                     GGTGASEGSKATTVTPGPWHIHRMLEAAEGLPINVGFTGKGQATNPTALIEQINAGAI
                     GLKVHEDWGATPSALSHALDVADEFDVQIALHADTLNEAGFMEDTMAAVKDRVLHMYH
                     TEGAGGGHAPDLIKSAAFSNILPSSTNPTLPYTHNTVDEHLDMVMITHHLNAAIPEDI
                     AFADSRIRKETIAAEDVLQDMGVFSMISSDSQAMGRVGEVITRTWQVAHRMKEQRGPL
                     DGDFEHNDNNRIKRYIAKYTINPAITHGISEYVGSIEPGKLADIVLWDPIFFGVKPEL
                     VVKGGLINSAVNGDANGSIPTSEPMKYRKMYGQYGGNLTSTSMTFVSKTAYENGINRA
                     LNLKRMVRPVKNIRQLSKADMKNNSATPKLDVDPQTYEVYVDGEKITSNAATELPLTQ
                     RYFLF"
     gene            complement(8760..9170)
                     /locus_tag="O0293_001590"
     CDS             complement(8760..9170)
                     /locus_tag="O0293_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HCY0280476.1"
                     /translation="MIPGEIITKSTEVEINNHHPETVIEVENTGDRPIQVGSHFHFYE
                     ANAALDFEREMAYGKHLDIPAGAAVRFEPGDKKEVQLVEYAGKRKIFGFRGMVNGPID
                     ESRVYRPTDENDEYAGVFGDNGAENVNKKGGKRS"
     gene            complement(9184..9486)
                     /locus_tag="O0293_001591"
     CDS             complement(9184..9486)
                     /locus_tag="O0293_001591"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HCY0280477.1"
                     /translation="MHFTQREQDKLMIVVAAEVARRRKARGLKLNHPEALALISDELL
                     EGARDGKTVAELMSYGRQILNKEDVMDGVEHMITDIEIEATFPDGTKLITVHHPIV"
     gene            9746..10651
                     /gene="yut"
                     /locus_tag="O0293_001592"
     CDS             9746..10651
                     /gene="yut"
                     /locus_tag="O0293_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HCY0280478.1"
                     /translation="MKIIDVLLKNISQVVLISNKWTGLFILIGLFVADWTIGLAAIVG
                     SIIAYTFARFINYSEAEINDGLAGFNPVLTAIALTIFLDKSGLDIVITMIATLLTLPV
                     AAAVREVLRPYKVPMLTMPFVIVTWFTILLSGQVKFVDTSLKLMPQNIETVNFSNNDR
                     IHFIQSLFEGFSQVFIEASVIGGVCILIGILIASRKATLLAVIASLLSFIIVALLGGN
                     YDDINQGLFGYNFVLMAIALGYTFKTAINPYISTFLGVLLTVVVQLGTTTLLEPFGLP
                     ALTLPFIIVTWILLFAGIKHDKVDA"
     gene            10868..>12001
                     /locus_tag="O0293_001593"
     CDS             10868..>12001
                     /locus_tag="O0293_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HCY0280479.1"
                     /translation="MNDMDNSFLITTEIQRKWIEKFKVIRDTFKAKAEYNDQHSQFPY
                     KNIEWLIKEGYGKLTLPKAYGGEGATIEDMVILQSFLGELDGATALSIGWHVSVVGQI
                     YEQKLWSQDMLEQFAVEINNGALVNRAVSEAEMGSPTRGGRPSTHAVKADDGYILNGV
                     KTYTSMSKALTHIIVAAYIEELESVGFFLVDRNLPGVEIADNWDVLGMRATESHDLIL
                     NDVKVPLKHLVETEKSKAPNGWILHIPSCYLGIAQAARNYAVDFAIQHSPNSIEGTIA
                     TLPTVQQNLGKMETLLLCARQFLWSTAKGYQQYKDDSQIRNPTSASKVMVMNQGLEVI
                     DLAMRIVGAKSLEMNRPLQRYYRDMRAGLHNPPMEDAAYTNIAK"
BASE COUNT         4139 a         1783 c         2004 g         4075 t
ORIGIN      
        1 tttcgaaaat aagtctgtta caaatttgta atattactga aaattctaaa tgtatatttt
       61 gtgcataata taggactttt aatcagaaaa tgcataaaaa ttcgacacga acgatcaagt
      121 ttctatgatt atgaaagctt tggtatttgg atgaaaatgt atatttaatg gataaaatat
      181 cctaatttag cataaaaaaa tgttttaata aaagtattat ttgatataat cgatttatgt
      241 tttgttactg ctaaaaaaca tgtggcgata taattggtag tgaatattag atgttgacaa
      301 ttgatttgag aaagacaaac aggagtaaat caatatattt gtatatgtga ggatgtaaga
      361 tgacagataa ttattcacta catatttata aaagtatgaa aatgagtagt gtgaaggaag
      421 cgaacttaaa aattatatat tggttacaag gtacaggtgt agtttcaatc aatctacaac
      481 aatatgatct aaaaagaaat tatgtcacca ttattatgtt gaatgatttg tatcaaattg
      541 atggtaatga tgatagtgta tgttgtgttg ttgaagtaag tgcaaagaca tttttgaagt
      601 ttatgaatgc aaactatatg atgcgtggca agatattaac aaatgaggtt gctagcactt
      661 ttagaatctt gattaagtat ttaatctaca gcaaaattaa acaacagtct tataatgcta
      721 atgataagat tattaattac atgtgtgttg aattaatgtc attaaataca tacgatggta
      781 gtcgtcattt agtagccgaa gaagtgcacg attatttaac aaacaaccac cataaaaaga
      841 taaataggaa agatgtcatt aatcaagtta gtattacaaa caaagcttta agtgacatgt
      901 ttaaagcgac accttatagt aattttattc aatatttaaa tcatattaga ctggagcatt
      961 gcttaattga tattttaact tctaagaagc ctattgaaga aatcgcaagt agtcatggtt
     1021 ttaatcatta ttctcgtttt atacacctct ttaaagaaac gtatggtaac actcctaaat
     1081 taattagaaa aagatatagt ccaacatctc attcaattaa ccattcccaa ttagttgaaa
     1141 ttgataaaaa tataattgaa ttatttgatg atactaatca aagttcttca agtatgcgcg
     1201 agattgatat accttttgag cctacaaaaa ggagtcaaat gtatcaacct aaaaacattt
     1261 atattaaagg aagtaatttt tattggatgg attattatac tattaaacaa atcattcaaa
     1321 gattaggtgt taatcctgaa aacttgcata taattgttac aatcgacaca agcaatgagc
     1381 tatcgattga acaaatcact ttattacttc aaaagatagt gaattacaat gtaaatgtag
     1441 tgtttcgtat taaacatgaa tataaggaat tgttaacgag tgctgaaaga ggcaagatag
     1501 aaaagctagt agctacaatt tttaatatga cttttgagag taatagatgt aagattgcat
     1561 ttttaattaa cgacttaaat actactgcaa ttcaaaaact gaaacgttta atagataatt
     1621 atataggagt atttgaatta atatatcatc tggaaccaaa agagttgaat acgcgctatg
     1681 ataaagaaat tgatcatcta atagactatt ttatcttacc gctaagacaa atagaacagg
     1741 tgtctattgc acgttctaaa attatatgtg atgtagattt gattaatgag aataatgact
     1801 taaaagaaaa aatcacaatg tctgatatta ataaggtttc tgaacttttg acaaattata
     1861 caaaatttcg aggacttatt atgcacatgt caattataag tgaatcgagc aaagaaataa
     1921 atttaactag attatcaata ttcattttta tcataaatat acttaatcaa ttacgaggga
     1981 ttgttattta tcaaaatgac acgatgattg taacgaagtt caaacatgaa gtacaatgtg
     2041 tcatttgttt gccagccgca ttgttaaatg agaattcaga cgctatacgt ttatcgtgta
     2101 agggtcttca acaatttagc catgctaaaa ctaaacaaat tgttttaagt atagataaag
     2161 ataatcatac ttccgaaaat ggtgtacttg tgaatgatat tatgcaacaa cacagttatt
     2221 ggaaagctaa ttttatagaa agaaatattt atgatcattc attaatggtc tcaagcaata
     2281 gtattgttca tttgaaatat catcttgaga tataggcatg gtttaagaga ggttttaaag
     2341 atgtctaaat ttaaagttaa gcgtttaact gcacacatac taatgttgaa agtcattaat
     2401 gatatgttga aatattcatt tcatctcact ttgactcaag tgaaactatt aaatctatta
     2461 gttaaatatg ataattctga ttccaatagc gcttcaatag atagtttatt aaaaatgaaa
     2521 gaaatccaat ctaagatggc ccttcttcaa atgttaagtt atttaaacaa tcatcaatgg
     2581 ctattgaaag gaagagatca aagggatcaa cgtaaattga ctatttcgat taataaaatg
     2641 catatagata aaatagaata tatgaataat gagcttagtg actatattga acgttatttt
     2701 ggtcaatata catttgattt tagttgtgac tatgtatcgt atttattgtc gtcacaagaa
     2761 ttattgcgga atctaaagtg ttatttaaat atgtgtcaat tatcattaga agagttatac
     2821 gttttaggaa tattaaatct gcataaaggt caattgacgg ttaaagaatt acagggtgaa
     2881 ttccatcacc cgatatttgc ggttagcccg attttaaagt gtttaatttt gaagggatta
     2941 gttaaaaaag agagatgtga attagatgaa aggcgtgtta ttgtaacaat caagcgagaa
     3001 aagttttcta aagtcactat gcttattcaa gcatgttata attatcttga aaagggaata
     3061 caagttaatc ttaataataa atagttaatc acttgtattt catataccta tgaagaatag
     3121 tttatagttt taagtgggag ggatattatg attttagata atgtgaatcc aaatgattta
     3181 tttccaactg aaaaaaaggg tccgtcagtg ctaggaataa ttgagtatca agttcaaggt
     3241 gagaatgaat ttgaaggggc atttatagca acaaatgaaa gattaattat gaatgtagat
     3301 atgaatggcc aattttatta tagaagtatt agttataatg aaatagagaa gatagattat
     3361 gatggtcaaa ctataatgtt caaatttaat attggtaatg tcccaatgca tgatattaaa
     3421 agtgctaacg ttgaaatgtt tgttgaatat gtaaaacaac atatgatagt ataatgtcaa
     3481 tgtatgtagg ttgataagaa gagtttgaat gataactcat tgcatttaaa gataaatggc
     3541 atgcgttatg atttaaagct cttctttttt tatattttat aaattttgtg agcaagccat
     3601 ccaattaata taaaaaatat caaatcaaac acgttgatat agattaaaat tttaaaagtt
     3661 tacatatcag ttaagataca aaatattcag actaataatt ttcaatttgg caaaatatct
     3721 taaacatcaa attattataa gaaataaatg tatttaacca tattctagta ataaaaatat
     3781 tgaattttta tacttatttg tttagaatga actttataac atagttggat agagtttcga
     3841 tttaataaat tacatgtgaa ccttgctaca acaagatgtg catcagagga gtggtttaat
     3901 aatgagtaaa attaatgaca ttaatgattt agtcaacgca acatttcaag ttaagaagtt
     3961 tttcagagat acaaaaaaga agttcaattt gaactatgaa gaaatttata ttttaaatca
     4021 tattttaaga agtgagtcta acgaaatctc atctaaagag attgctaagt gctcagagtt
     4081 caaaccttac tatttaacta aagctttaca aaagctaaaa gatttaaaat tgttatcaaa
     4141 gaaaagaagt ttacaagacg aaagaacagt tattgtttat gttacagata cacaaaaagc
     4201 aaatattcaa aaactgattt cagaattaga agaatacatt aaaaattaaa tcaaggttaa
     4261 ttgcgtttaa taacattgaa cgataacaat ttattaaaac gaagttattt attcagcatt
     4321 gggacataaa attaacttaa aatttaaata ttgaagatgc tttaattaaa gttaaagact
     4381 agccatacct tatttcagct tattaagctt gaaacaaggt acactagtct ttttatttta
     4441 atattttctt agaaaatcaa gtttacgatc ataaatattt tctgcgatat agctttggat
     4501 ggttccaagt attttctcta taatttgtgt gcgataagca aaaattctaa ctgcaaaacc
     4561 atgtgtaggc aattgagaaa tagcaacacg acaatcgaat gtattgctat aagaactaat
     4621 ggtttcataa actgaatcga ttagtttttg gttaacatct tcgtgtatga agtaagcaga
     4681 gccataatga gagtaatgtt ccatataacc tatttcattg atcgattgtt tattaggatt
     4741 caataataaa ttatcatatg tgactaactc atcttcaata taaatttcat ttattaaatg
     4801 catatattga tacttgaagg cttcgccagt ttttgaataa ccaggagtta aaatatcagt
     4861 ataaaataat gaactagaat tattgagatt gaacgtattg tgttgataaa atttagcatt
     4921 ttcatatgcg atgattggat cagcgacata ttctaaatat gcgttatctt tcaaattaaa
     4981 agtttgatac tgctcaacat gattggaagg tgtcttgtat atttttgttg caccttgaga
     5041 tgtcaatgtc actttagcgt tatcttcgac attcacattc atacggtaac gatctccatc
     5101 taaatagcca ccacctacat ttactatata aaatgtagga atggtagatt gatttagata
     5161 aactggacgt atcactttaa gtgctttttc aaagaaaata tctcttgata ctgatcgatt
     5221 gccatcgaaa aacactgtta aatcaagttg cccagtccat tgttgttcat ccattatgat
     5281 aatcctttga gtaaagtgtc gcgttcaatc caatcgataa cttcatccaa accttcgtcg
     5341 gtttttaagt tagtaaaagt aaatggacgt ttaccacgaa atactttagt atcttcagcc
     5401 atttgttcta atgatgcacc tacataggga gctaaatcag ttttgttaat tacaaagaaa
     5461 tctgacttaa tcataccttg accaccttta cgtggaatct tttcaccttg agcaacatcg
     5521 ataatatata ttgaaaagtc aacaagttct ggactaaatg ttgctgctaa gttatcgcca
     5581 ccagattcta tgaaaattag ttctatatcg tcatgacgtt ctaataattc gtctattgct
     5641 gcaaagttca tagatgcatc ttcacgaatc gcagtatgag gacatccacc agtttcaaca
     5701 ccaatgatac gactttcagg tagaactcct gaatttacta atatcttttc gtcttctttt
     5761 gtatatatat catttgtaat aacgccgata ctcatttctt ttgaaagacg ttttacaact
     5821 ttttcaatta attgtgtttt acctgcacct acaggaccac caataccaat tttaatcgga
     5881 tttgccacaa ttataacctc ctatgatatg aaaattctaa cattgacgtt ctcatgcgcc
     5941 atttgattta gttctaaacc aggcgctgtc atgccaaaat ctgcttcttt taattcgaaa
     6001 atctgctttc ttgttccttc tatataagga atcatgtgag taactatctt ttgaccagca
     6061 gtttgtccaa gtggaatagc acgaacagca ttttgagtta aacttgaaac attttgatat
     6121 aaatagtaat caataatcgt ttcaatatct acacctaaat gatggcctag catagtaaaa
     6181 caaatagctg gatttaactt tgctttctta tcttgcattt gttgatgata ccaagcaatc
     6241 catgggctat tataaagttc taaagccaat ttaaccatgc gagtccccat ttgttttgca
     6301 ccaacacgtg tttctttagg taagttttga acaaacatca gtttatctat gtgtaatact
     6361 ttttgtgtat catcattttc caatgcatca taaactaaac gcatggctaa accatcagaa
     6421 taggtaagtt gctcttgtaa aaacattttt aaccaagcaa taaaagtatg atcgtcatga
     6481 attatatttc gttgaatata tgtttcaaga ccaaatgaat gactgaaagc acctgttgga
     6541 aactgtgaat cacagaactg aaataatctt aagtgtgtat gatcaatcat gagaatgccc
     6601 tatatgtctg aaagccttat taactttacg gtcttctcga acatatggga tgcctaaact
     6661 ttttaataaa tcttcaacta aataatcata ttgtactagc atttcagtct ctgtaaattg
     6721 tgctggcaaa tgacgatttc ctaattgatg ggctatacct cccatttctt gcaatgttct
     6781 tggttgaatc actaaaagat cttctgaatt aacatccaca ataatcatat tatggtcatc
     6841 tgcgtataaa atatctccat attgtaagtc aataggttgt tttaaacgaa tgcctatttc
     6901 agtgccatgg tctgtaacga ctctttgaat acgtttaaca agatctgaat tttcaaggta
     6961 tactttttcg acgtgctttt gtttttctga atttgacaaa ttggcaatat tgccttggat
     7021 ttcttcaaca atcattctat gttcctccta gaataagaag tatctttgag ttaatggtaa
     7081 ctcagttgct gcattacttg taattttttc tccatctaca tatacttcat atgtttgtgg
     7141 atcaacgtct aatttaggtg ttgcactgtt atttttcata tctgctttag ataattgtct
     7201 aatattttta actggacgca ccatgcgttt taaatttaat gcacgattga taccattttc
     7261 ataagcagtt ttagacacga atgtcattga cgtacttgta aggtttccgc cgtattgacc
     7321 atacatttta cggtacttca tcggttcaga tgtaggtata gaaccatttg catcgccatt
     7381 tacggcagag ttaattaatc cgccctttac aactaattca ggtttaaccc caaagaaaat
     7441 tgggtcccat aagacaatgt cagctagttt gcccggctcg atagatccta catattcaga
     7501 aataccatgt gtaattgctg ggttaattgt atatttagcg atataacgtt tgatgcgatt
     7561 attatcatta tgttcaaaat caccatctaa aggaccacgt tgttctttca tgcgatgtgc
     7621 tacttgccat gttcgtgtaa ttacttcacc tacacggccc attgcttgtg aatcggaact
     7681 aatcatactg aatacaccca tatcttgcag aacatcttct gctgcaatcg tttctttacg
     7741 aatacgtgaa tctgcgaatg cgatatcttc aggaatagcc gcatttaaat ggtgagtaat
     7801 cattaccata tctaaatgtt catctacagt attatgtgta taaggcaaag ttggatttgt
     7861 agatgaaggt aaaatatttg aaaatgcagc ggatttaatt aaatcaggcg catgaccgcc
     7921 accagcacct tcagtatggt acatatgaag tacacggtct ttaacagcag ccattgtgtc
     7981 ttccataaat cctgcttcat ttaaagtatc tgcatgtaat gcaatttgaa catcaaattc
     8041 atcagcaaca tctaatgcat gactcaaagc agatggtgtt gcaccccagt cttcatgtac
     8101 ttttaatcca attgctccgg cattgatttg ttcaatgagt gcagttggat ttgttgcttg
     8161 tcctttacct gtaaaaccga cattaatcgg taaaccttcg gcagcttcta acattctatg
     8221 aatatgccat ggacctggag ttacagttgt tgctttagaa ccttctgaag caccagtacc
     8281 accaccaata tgagtcgtaa taccactttc taatgcgacc tctgcttgtt caggattaat
     8341 aaaatgaaca tgagtatcaa taccaccagc agtgacgatt ttaccttcag cggcaatgat
     8401 atctgttgtt gaacctataa taatgtcgac attatccatt atatctgggt tgccggcatt
     8461 acctatggcg aaaatataac catttttaat gcctatatca gctttaacca ctttatcgta
     8521 atcgataata acggcattag aaatgacaag gtctgcaacg ttcacgtcat cacgtgttac
     8581 acgaggattt tgcgccatac cgtctctaat agatttacca ccaccaaaag tagcttcttc
     8641 accataaacc gcatagtctt tttctatttg agcaaataga ttcgtatcac ctaaacgaat
     8701 ggaatctcca acagttggac cgtataagct cgtatattga ttttgcgtca ttttaaagct
     8761 catgatcttt ttcctccttt tttattcacg ttttcagcac cgttatctcc gaatacacct
     8821 gcatattcat cattttcatc agttgggcga tagacacgtg actcatcgat aggaccattg
     8881 accataccac gaaaaccaaa aattttacgt ttgccagcat attcaactaa ttgaacttct
     8941 tttttatccc caggttcaaa tcgaacagct gctccagctg gaatatctaa atgttttcca
     9001 tatgccattt cacgttcgaa atctaatgct gcatttgctt cataaaaatg aaaatgtgag
     9061 cccacttgaa ttggtcgatc tcctgtattt tcaacttcga taactgtttc aggatgatgg
     9121 ttattaattt caacctctgt actttttgta ataatttctc ctggtatcat ttgactgcct
     9181 cctttaaaca atagggtgat gtactgtgat taacttagta ccatcgggga acgtagcctc
     9241 gatttcgata tctgtaatca tgtgttcgac accatccatg acatcttctt tgtttagaat
     9301 ttgtctacca taactcatta actctgcaac ggtcttacca tcgcgtgcac cttctaataa
     9361 ttcatcgctg attaaagcta atgcctcagg atgatttagt ttcaaaccac gtgctttacg
     9421 acgacgtgca acttccgccg ccactacaat cattaatttg tcttgctctc gttgtgtaaa
     9481 atgcaaatta aaacccccaa tttcatatta gatacaattt acaaaattta tattaatcct
     9541 aattgttgtg ataaacaagt aatatacaaa gttcaatgtg taattagaaa attatatttt
     9601 tagcatatcc gatattgaag caaacaatct aatcgaaaac aaatagtgga atatatttat
     9661 gtaaaaacca aaatagtttt taatataact tttcatagaa tagtagtata ttaatgagta
     9721 atgattcaaa ggaaaggtga aagatttgaa gataatagat gtgcttttga aaaatatatc
     9781 tcaggttgtg ttaattagta ataaatggac aggattattt atcttaatag gattatttgt
     9841 agccgattgg acaattggat tagcggctat tgtaggtagc atcatcgcct atacttttgc
     9901 gcgttttata aattatagtg aggcagagat taatgatggg ttagctggat ttaatccagt
     9961 gctaactgcc attgcgttaa caatcttttt agataagtca ggattagata ttgttataac
    10021 aatgatagca actttattaa cgttaccagt tgctgctgca gtgagagaag ttttaagacc
    10081 atataaagtt ccgatgctga cgatgccttt tgtcattgtg acttggttta caattttact
    10141 ttcaggacag gttaaatttg tagatacatc gttaaagtta atgcctcaaa acattgaaac
    10201 ggttaatttt agcaacaatg atagaataca tttcattcag tcattatttg aaggattcag
    10261 tcaagtattt atcgaagcga gtgtaattgg tggcgtatgt attttaatcg gcatattgat
    10321 agcatcaaga aaagcaacac tcttagctgt tatagctagt ttgttaagct ttatcattgt
    10381 agctctatta ggtggtaatt atgatgatat taatcaggga ttattcggtt ataactttgt
    10441 attaatggca atcgcactag gatatacatt taaaacagcg attaaccctt atatttcgac
    10501 ttttttaggt gtgttattaa cagtagtggt gcaactaggt acaacaacat tgcttgaacc
    10561 gtttggctta cctgcattaa cattgccatt tattatcgtg acatggattt tattatttgc
    10621 tggtattaaa catgacaaag tagatgcttg atagttaaat caaacctaat attgtttgaa
    10681 tatcacctta aactatacag cgaattgtat agtttaaggt gtatttttat ggataaaatt
    10741 aagtgcatac ttaatcgaat tatcagtttt cttttaattg ttcacagcac gttccttttt
    10801 atttttaaag tcgagtgtta aactaggaat aaataattta tattgtgtgt tgtgtggggt
    10861 gactaatatg aatgatatgg ataattcctt tttaataaca acggaaattc aaagaaaatg
    10921 gattgaaaaa ttcaaagtaa ttagagatac atttaaggct aaagctgaat ataatgatca
    10981 acatagccaa tttccatata aaaatattga atggttaatt aaagaaggtt atggaaaatt
    11041 aacgttacca aaagcatatg gtggtgaagg tgcgaccata gaagacatgg ttattttgca
    11101 atcattttta ggcgaacttg atggtgccac agcattatct attggttggc atgtgagtgt
    11161 cgtaggacaa atttatgaac agaaattatg gtctcaagat atgttggagc aatttgctgt
    11221 tgaaattaat aatggtgcat tagttaatag agcagttagt gaagctgaaa tgggtagtcc
    11281 aacaagaggg ggaagaccaa gtacacatgc tgttaaagct gatgatgggt atattttaaa
    11341 tggtgtgaag acatatacat caatgagtaa agcactaaca catattattg ttgctgctta
    11401 tatagaagaa ttagaaagtg ttggtttttt cttagtagac aggaatttgc ctggtgttga
    11461 aattgccgat aattgggacg tattaggtat gcgagcgaca gaaagtcatg atttaatatt
    11521 aaatgatgta aaagtaccat taaaacattt agtggaaaca gagaaaagta aagcgccaaa
    11581 tggttggata ttgcatatac caagctgtta tttaggtata gcacaagctg ctagaaatta
    11641 tgctgtagac tttgcaatcc aacatagccc taatagtatt gaaggaacga ttgcaacatt
    11701 gccaactgtg caacaaaatt tagggaaaat ggaaacgcta ttgttatgtg cgagacaatt
    11761 tttatggagt acagcaaaag ggtatcaaca atataaagat gacagtcaga taagaaatcc
    11821 aacaagtgcg agtaaagtga tggtaatgaa tcaagggctt gaagtgattg atttagcgat
    11881 gagaatagtt ggtgctaaaa gcttagaaat gaatcgacca ttacaacgtt actatcgtga
    11941 catgcgtgca ggattacaca atccacctat ggaagatgca gcatatacaa atattgctaa
    12001 g
//
LOCUS       DAKTIO010000081        11987 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.081, whole genome shotgun sequence.
ACCESSION   DAKTIO010000081 DAKTIO010000000
VERSION     DAKTIO010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11987
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.081"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            127..2223
                     /gene="pnp"
                     /locus_tag="O0293_001594"
     CDS             127..2223
                     /gene="pnp"
                     /locus_tag="O0293_001594"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCY0280480.1"
                     /translation="MSQEKKVFKTEWAGRSLTIETGQLAKQANGAVLVRYGDTVVLST
                     ATASKEPRDGDFFPLTVNYEEKMYAAGKIPGGFKKREGRPGDDATLTARLIDRPIRPL
                     FPKGYKHDVQIMNMVLSADPDCSPQMAAMIGSSMALSVSDIPFQGPIAGVNVGYIDGK
                     YIINPTVEEKEVSRLDLEVAGHKDAVNMVEAGASEITEQEMLEAIFFGHEEIQRLVDF
                     QQQIVDHIQPVKQEFIPAERDEALVERVKSLTEEKGLKETVLTFDKQQRDENLDNLKE
                     EIVNEFIDEEDPENELLIKEVYAILNELVKEEVRRLIADEKIRPDGRKPDEIRPLDSE
                     VGILPRTHGSGLFTRGQTQALSVLTLGALGDYQLIDGLGPEEEKRFMHHYNFPNFSVG
                     ETGPVRAPGRREIGHGALGERALKYIIPDTADFPYTIRIVSEVLESNGSSSQASICGS
                     TLALMDAGVPIKAPVAGIAMGLVTREDSYTILTDIQGMEDALGDMDFKVAGTKEGITA
                     IQMDIKIDGLTREIIEEALEQARRGRLEIMNHMLQTIDQPRTELSAYAPKVVTMTIKP
                     DKIRDVIGPGGKKINEIIDETGVKLDIEQDGTIFIGAVDQAMINRAREIIEEITREAE
                     VGQTYQATVKRIEKYGAFVGLFPGKDALLHISQISKNRIEKVEDVLKIGDTIEVKITE
                     IDKQGRVNASHRALEE"
     gene            2459..4132
                     /locus_tag="O0293_001595"
     CDS             2459..4132
                     /locus_tag="O0293_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCY0280481.1"
                     /translation="MSLIKKKNKDIRIIPLGGVGEIAKNMYIVEVDDEMFMLDAGLMF
                     PEDEMLGIDIVIPDISYVLENKDKLKGIFLTHGHEHAIGAVSYVLEQLDAPVYGSKLT
                     IALIKENMKARNIDKKVRYYTVNNDSIMRFKNVNISFFNTTHSIPDSLGVCIHTSYGA
                     IVYTGEFKFDQSLHGHYAPDIKRMAEIGEEGVFVLISDSTEAEKPGYNTPENVIEHHM
                     YDAFAKVRGRLIVSCYASNFIRIQQVLNIASKLNRKVSFLGRSLESSFNIARKMGYFD
                     IPKDLLIPITEVDNYPKNEVIIIATGMQGEPVEALSQMAQHKHKIMNIEEGDSVFLAI
                     TASANMEVIIANTLNELVRAGAHIIPNNKKIHASSHGCMEELKMMINIMKPEYFIPVQ
                     GEFKMQIAHAKLAAEAGVAPEKIFLVEKGDVINYNGKDMILNEKVNSGNILIDGIGIG
                     DVGNIVLRDRHLLAEDGIFIAVVTLDPKNRRIAAGPEIQSRGFVYVRESEDLLREAEE
                     KVREIVEAGLQEKRIEWSEIKQNMRDQISKLLFESTKRRPMIIPVISEI"
     gene            4388..6757
                     /locus_tag="O0293_001596"
     CDS             4388..6757
                     /locus_tag="O0293_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCY0280482.1"
                     /translation="MAQAKKKSTAKKKTTSKKRTNSRKKKNDNPIRYVIAILVVVLMV
                     LGVFQLGIIGRLIDSFFNYLFGYSRYLTYILVLLATGFITYSKRIPKTRRTAGSIVLQ
                     IALLFVSQLVFHFNSGIKAEREPVLSYVYQSYQHSHFPNFGGGVLGFYLLELSVPLIS
                     LFGVCIITILLLCSSVILLTNHQHREVAKVALENIKAWFGSFNEKMSERNQEKQLKRE
                     EKARLKEEQKARQNEQPQIKDVSDFTEVPQERDIPIYGHTENESKSQSQPSRKKRVFD
                     AENSSNNIVNHHQADQQEQLTEQTHNSVESENTIEEAGEVTNVSYVVPPLTLLNQPAK
                     QKATSKAEVQRKGQVLENTLKDFGVNAKVTQIKIGPAVTQYEIQPAQGVKVSKIVNLH
                     NDIALALAAKDVRIEAPIPGRSAVGIEVPNEKISLVSLKEVLDEKFPSNNKLEVGLGR
                     DISGDPITVPLNEMPHLLVAGSTGSGKSVCINGIITSILLNAKPHEVKLMLIDPKMVE
                     LNVYNGIPHLLIPVVTNPHKAAQALEKIVAEMERRYDLFQHSSTRNIKGYNELIRKQN
                     QELDEKQPELPYIVVIVDELADLMMVAGKEVENAIQRITQMARAAGIHLIVATQRPSV
                     DVITGIIKNNIPSRIAFAVSSQTDSRTIIGTGGAEKLLGKGDMLYVGNGDSSQTRIQG
                     AFLSDQEVQDVVNYVVEQQQANYVKEMEPDAPVDKSEMKSEDALYDEAYLFVVEQQKA
                     STSLLQRQFRIGYNRASRLMDDLERNQVIGPQKGSKPRQVLIDLNNDEV"
     gene            6762..7475
                     /locus_tag="O0293_001597"
     CDS             6762..7475
                     /locus_tag="O0293_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCY0280483.1"
                     /translation="MSEMNAVYNVKQYILNLIKQNKLEYGDQLPSNLSIARELNVKTD
                     DVYEAIQALITEQVIKDNFEEGTSVKSLPPFFYPLNELISIGQMIKNAGFECGTEYLN
                     FDEQPATMLDANLLSVEEGYPVTIIERLRTADGEPVVYCLDKIAKKELTCTEYQMSNG
                     SILSAIKEQSNHNICYADTEIEAVNYEPRISEVLNASPHEGLILLKITHYNESDEPIL
                     YSLNYMKNSLVQFKITRKI"
     gene            7506..8771
                     /locus_tag="O0293_001598"
     CDS             7506..8771
                     /locus_tag="O0293_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCY0280484.1"
                     /translation="MSRQSQPNIHIKVSPTTKFKTTTIVFKFMAPLEYDTITARSLLS
                     KLLVRATKKWPTDKSFNNHLADLYGAYVNSTISKFKDQHVITFSLEIVNERYLRNGES
                     LFNQGLDLLQEIIWNPLIENKAFNDNFVNQEKTLLAKKIEAMVDNKAQYSFLKLLDHM
                     FENEAYKYLSTGQLEQIPHITAETLYHTYQSMINNDQCSVYVVGNVEPESVEKQIREK
                     FALKPFDKHQFQHSTHHLHDEEVDYIVEYDDVDQAKLNMGYRFPTQYGQSGYAAFVVF
                     NMMFGGDPSSVLFNEVREKQSLAYSIHSQIDGKNGYLFVLSGVSSDKYETAKDTIISE
                     FEKIKAGDFTEEKLELAKKVIISHRYESEDRPKSIIEIMHNQILLEQPQSKETFINDI
                     QKVSREDIVSVAEKAFLDTIYVLTKGGDK"
     gene            8771..10057
                     /locus_tag="O0293_001599"
     CDS             8771..10057
                     /locus_tag="O0293_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCY0280485.1"
                     /translation="MKERYYELIDERVFEQELENGLRLFIIPKPGFQKTFVTYTTQFG
                     SLDNQFKPLGQDQFVTVPDGVAHFLEHKLFEKEEEDLFTAFAEDNAQANAFTSFDRTS
                     YLFSATDNIENNIKRLLTMVETPYFTKETVDKEKGIIAEEIKMYQEQPGYKLMFDTLR
                     AMYQQHPIRVDIAGSVESIYDITKDDLYLCYETFYHPSNMVLFVVGDVDPEAICRIVK
                     QHEDARNKVNQPKIERGLVDEPEDVKEAFVTESMKIQSPRLMLGFKNKPLQEAPQKYV
                     QRDLEMSLFFELIFGEETDFYQNLLNEGLIDDTFGYQFVLEPTYSFSIVTSATEEPDK
                     LKKLLLDELRDKKGNFQDAEAFELLKKQFIGEFISSLNSPEYIANQYTKLYFEGVSVF
                     DMLDIVENITLDSINETSSLYLNLDQQVDSRLEIKK"
     gene            10057..10761
                     /locus_tag="O0293_001600"
     CDS             10057..10761
                     /locus_tag="O0293_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCY0280486.1"
                     /translation="MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFN
                     DDKVRFIQADLSQTIDIDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNV
                     LQLIRLCRYFVDVLRQSANGRIIVISSIWGETGASMETIYSAMKSAQLGFVKALSQEL
                     ALTSVTVNAIAPGFVAGNMASEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHPNA
                     RSVTGTIQKVNGAWYI"
     gene            10866..11693
                     /locus_tag="O0293_001601"
     CDS             10866..11693
                     /locus_tag="O0293_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfK family protein"
                     /protein_id="HCY0280487.1"
                     /translation="MTVAEKKEWYLEYEIEINRPGLLGDVSSLLGMLGISIVTINGVD
                     QGKRGLLIKTDNLEKVERFEQIARGINEIEITKLKKPELRDRLAVRHGRYIEQDAKDK
                     KTFRFEREDLGLLVDFLAELFKEEGHKLIGIRGMPRVGKTESIVAGSVCAHKRWLFIS
                     STLIKQTVRSSLIKGEYDANHVYIIDGAVTARESNPKHQELVNEVMTLPSIKVVEHPD
                     LFVETSTCTMEDFDYIIELRENENQEIHYEEMKKQTVQSKNNLDFGDPFGGGFGFFE"
     gene            11712..>11987
                     /locus_tag="O0293_001602"
     CDS             11712..>11987
                     /locus_tag="O0293_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCY0280488.1"
                     /translation="MKTVGEALKGRRERLGMTLTELEQRTGIKREMLVHIENNEFDQL
                     PNKNYSEGFIRKYASVVNIEPNQLIQAHQDEIPSNQAEWDEVITVFNN"
BASE COUNT         4388 a         1635 c         2325 g         3639 t
ORIGIN      
        1 ctattttgta aataatagat ggatatcccg ctctcttttt ttccaattca atattttata
       61 actaatatta aaatacgata ataaatgata tgatataact attagattca agagaggaga
      121 tttataatgt ctcaagaaaa gaaagttttt aaaactgaat gggcaggaag atctttaacg
      181 attgaaacag ggcaattagc taaacaagca aatggcgctg tattggttcg ttatggagat
      241 acagtcgtgt tatcgacggc aactgcatca aaagaacctc gtgatggaga tttcttccca
      301 ttaacagtga actatgaaga aaaaatgtac gctgcgggta aaattcctgg tggatttaaa
      361 aagagagaag gacgtcctgg tgacgatgca acattaactg cgcgattaat tgatagacca
      421 attagacctt tattccctaa aggatataag catgatgttc aaattatgaa catggtatta
      481 agtgcagatc ctgattgttc accacaaatg gctgcaatga ttggttcatc tatggcgctt
      541 agtgtgtcgg atattccatt ccaagggcca atcgccggtg taaatgtggg ttatattgac
      601 ggtaaatata tcattaaccc aacagtagaa gaaaaagaag tttctcgttt agaccttgaa
      661 gtagctggtc ataaagatgc ggtaaacatg gtagaggcag gcgctagtga gattactgaa
      721 caagaaatgt tagaggcgat tttctttggt catgaagaga ttcaacgttt agttgatttc
      781 caacaacaaa tcgtcgacca cattcaacct gttaaacaag aatttattcc agcagagcgt
      841 gatgaagcgc tagttgaacg tgtaaaatct ttaaccgaag aaaaaggact taaagaaaca
      901 gttttaacat ttgataaaca acaacgagat gaaaatcttg ataacttaaa agaagaaatc
      961 gtcaatgaat ttatcgatga agaagatcca gagaatgaat tacttattaa agaagtttat
     1021 gcaattttaa atgaattagt gaaagaagaa gttcgacgtt taattgcaga tgaaaaaatt
     1081 agaccagacg gccgtaaacc tgatgaaatc cgtccattag attctgaagt tggtatttta
     1141 cctagaacgc atggttcagg tctatttaca cgtggtcaga ctcaagcact ttcagtttta
     1201 acattaggtg ctttaggcga ttatcaatta attgatggtt taggacctga agaagaaaaa
     1261 agattcatgc atcattacaa cttcccgaat ttttcagtag gtgaaactgg tccagtacgt
     1321 gcgccaggtc gtcgtgaaat tggacatggt gcgttaggtg aaagagcatt aaaatatatt
     1381 attcctgata ctgctgattt cccatataca attcgtattg taagtgaggt acttgaatca
     1441 aatggttcat catctcaagc gtcaatttgt ggatcaacat tagcattaat ggatgcgggc
     1501 gtaccgatta aagcaccagt tgctggtatt gctatgggcc ttgttacacg tgaagatagc
     1561 tatacgattt taactgatat ccaaggtatg gaagatgcat taggtgatat ggactttaaa
     1621 gtcgctggta ctaaagaagg tattacagca atccaaatgg atattaaaat tgacggttta
     1681 acgcgtgaaa ttatcgaaga ggctctagaa caagcgagac gtggtcgttt agaaataatg
     1741 aatcatatgt tacaaacaat tgatcaacca cgtactgaat taagtgctta cgcgccaaaa
     1801 gttgtaacta tgacaattaa accagataag attagagatg ttatcggacc tggtggtaaa
     1861 aaaattaacg aaattattga tgaaacaggt gttaaattag atattgaaca agatggtact
     1921 atctttattg gtgctgttga tcaagctatg ataaatcgtg ctcgtgaaat cattgaggaa
     1981 attacacgtg aagcggaagt aggtcaaact tatcaagcca ctgttaaacg tattgaaaaa
     2041 tacggtgcgt ttgtaggcct attcccaggt aaagatgcgt tgcttcacat ttcacaaatt
     2101 tcaaaaaata gaattgaaaa agtggaagat gtattaaaaa tcggtgacac aattgaagtt
     2161 aagattactg aaattgataa acaaggtcga gtaaatgctt cacatagagc attagaagaa
     2221 taatatttaa agtcatatga cgacaatgta tcgtcatgtg atttttttat gccacttttt
     2281 acgaagtgac ccgttttgaa tttgttgtat tgaacatttt aaaacgcttt attattttgt
     2341 gtgcaactgt taattatcct gtatgtatag tgattaatag tgtacatcaa gtgtttttta
     2401 acttataatg aatagtgagt ttatatatgg acgggtaaca aatttaggag gtaagatttt
     2461 gagtttaata aagaaaaaga ataaagatat tcgcattata ccattaggcg gtgttggcga
     2521 aattgctaaa aatatgtata tcgttgaagt agacgatgaa atgtttatgt tagatgctgg
     2581 acttatgttt ccagaagacg aaatgctagg tattgatatt gttataccag acatttcata
     2641 cgtacttgaa aataaagata aattgaaggg tatattcctt acacacggac atgagcacgc
     2701 gattggtgca gtgagttatg ttttagaaca attagatgca ccagtatatg gatctaaatt
     2761 gacaatagcg ttaattaaag aaaatatgaa agcccgtaat attgataaaa aagttcgcta
     2821 ctatacagtt aataatgatt caattatgag attcaaaaac gtgaatatta gtttctttaa
     2881 tacgacacac agtattcctg atagtttagg tgtttgtatt cacacttcat atggtgccat
     2941 tgtgtataca ggtgaattta agtttgacca aagtttacat ggacattatg caccagatat
     3001 taaacgtatg gcagagattg gtgaagaagg cgtatttgtc ttaatcagtg attctactga
     3061 ggcagagaaa cctggatata atactccgga aaatgtgatt gaacatcata tgtatgatgc
     3121 ttttgcaaaa gtgcgaggtc gcttgatagt ttcatgttat gcttcgaact ttatacgtat
     3181 tcagcaagtt ttaaatattg ctagcaagct aaatcgtaaa gtgtcatttt taggaagatc
     3241 acttgaaagt tcatttaata ttgctcgtaa aatggggtat ttcgacattc ctaaagattt
     3301 gctaattcct ataacagaag ttgataatta tcctaaaaat gaagtgataa ttatagctac
     3361 tggtatgcaa ggagaacctg tagaagcctt aagtcaaatg gcgcaacata agcataaaat
     3421 tatgaatatc gaagaaggcg attctgtatt tttagcaatt acggcttctg ctaatatgga
     3481 agttatcatt gcgaatacat taaatgagct tgtacgtgct ggcgcacata ttattccaaa
     3541 taacaaaaag attcatgctt caagtcatgg ttgcatggaa gaattaaaaa tgatgattaa
     3601 tattatgaaa cctgaatact ttattcctgt acaaggtgaa tttaaaatgc agatagcaca
     3661 tgcgaagcta gcagctgaag caggtgttgc accagaaaag attttccttg tggaaaaagg
     3721 agatgtcatt aattacaacg gtaaagatat gatattaaat gaaaaggtaa attcaggaaa
     3781 tattttaata gatggcattg gtattgggga tgtaggaaat atcgtgttga gagaccgtca
     3841 tcttttagca gaagatggta tctttattgc tgttgtaacg ttagatccta aaaatagacg
     3901 tatagctgcg ggacctgaaa ttcaatctcg tgggtttgta tatgtacgtg aaagtgaaga
     3961 cttattacgt gaagcagaag agaaagtacg tgaaatagta gaggctggtt tacaagaaaa
     4021 acgcatagaa tggtctgaaa ttaaacaaaa tatgcgtgat caaattagta aactattatt
     4081 cgaaagtaca aaacgtcgtc ctatgattat tccagtaatt tctgaaattt aatcaaaaag
     4141 tcattaacat aaaagaggtc agaacaagtc actgaaatat aatggttgtc atggacaatt
     4201 tacttatatt ttatgatggt caattgaagg ggtaacgatt aatctgttat cttaagtaaa
     4261 ttgatacata gatgatattg ttctaacctc tttcatcgtc tgtttggact acatattcta
     4321 aacatcaaat aggaaattat atataataac gtcgttttaa ctaaggcaac ataaggaggt
     4381 gcgtcaattg gcacaagcaa aaaagaaatc gacagctaag aaaaaaacaa catcaaaaaa
     4441 aagaacaaat tcgaggaaaa agaagaatga taatccgata cgttatgtca tagctatttt
     4501 agtagttgta ttaatggtgt tgggtgtttt ccaattagga ataataggtc gtctaattga
     4561 cagcttcttt aattatttat ttgggtacag tagatattta acatatattt tagtactctt
     4621 agcaactggt tttattacat actctaaacg tattcctaaa actagacgaa cggctggttc
     4681 gattgtattg caaattgcat tgctatttgt atcacagtta gtttttcatt ttaatagtgg
     4741 tatcaaagct gaaagagaac ctgtactttc ttatgtgtat cagtcatacc aacacagtca
     4801 tttcccaaat tttggtggcg gtgtattagg cttttattta ttagagttaa gcgtaccttt
     4861 aatttcatta tttggtgtat gtattattac tattttatta ttatgctcaa gtgttatttt
     4921 attaacaaac catcaacatc gtgaagttgc aaaagttgca ctggaaaata taaaagcttg
     4981 gtttggttca tttaatgaaa aaatgtcgga aagaaaccaa gaaaaacaat tgaagcgtga
     5041 agaaaaagca agacttaaag aagaacaaaa ggcacgtcaa aatgaacagc cacaaataaa
     5101 agatgtgagt gattttacgg aagtgcctca agaaagagat attccaattt atgggcatac
     5161 tgaaaatgaa agtaaaagcc agagtcaacc aagtcgaaaa aaacgagtgt ttgatgcaga
     5221 gaatagttcg aataacatcg taaatcatca tcaagcagat cagcaagaac aattaacaga
     5281 acaaactcat aacagtgttg aaagtgaaaa cactattgaa gaagctggtg aagttacgaa
     5341 tgtatcgtat gttgttccac cgttaacttt acttaatcaa cctgcaaaac aaaaagcaac
     5401 atctaaagct gaagtgcaac gtaaaggaca agtactagag aatacattaa aagattttgg
     5461 ggtaaatgca aaagtgacac aaattaaaat tggtcctgca gtaactcaat atgaaattca
     5521 accagctcaa ggggttaaag tgagtaaaat tgtaaacttg cataatgata ttgcattagc
     5581 tttagcagca aaagatgtta gaatcgaagc gccaatacct ggtcgttctg cagtaggtat
     5641 tgaagtgcca aatgagaaaa tttcattagt ttcactaaaa gaagttttag atgaaaaatt
     5701 cccgtctaat aataaactag aagttggatt aggaagagat atatcaggtg atccaattac
     5761 tgttccacta aatgaaatgc cacacttatt ggtggcagga tcgacgggta gtggtaaatc
     5821 tgtttgtata aatggtatta ttacaagtat tttattaaat gctaagccgc atgaagttaa
     5881 acttatgtta atcgatccga aaatggttga actaaatgtt tataacggaa ttccacactt
     5941 attaattccg gttgttacaa atcctcataa agctgctcaa gctttagaaa aaattgtagc
     6001 tgagatggaa agacgttatg atttattcca acattcatca actagaaaca ttaaaggtta
     6061 taacgaatta atccgtaagc aaaatcaaga attagatgag aagcaaccag aattacctta
     6121 tatcgttgtt attgtagatg agcttgcaga tttaatgatg gtagctggta aagaagttga
     6181 aaatgcgatt caacgtatta cacaaatggc acgtgcagca ggtatacatt taattgtagc
     6241 gacacaaaga ccttctgtgg atgtaattac aggtatcatt aaaaataata ttccatctag
     6301 aatagctttt gctgtgagtt ctcaaacaga ttcaagaact attattggta ctggcggcgc
     6361 agaaaagtta cttggtaaag gtgacatgtt atacgttgga aatggtgact catcacaaac
     6421 acgtattcaa ggggcgtttt taagtgacca agaggtgcaa gatgttgtaa attatgtagt
     6481 agaacaacaa caggcaaatt atgtaaaaga aatggaacca gatgcaccag tggataaatc
     6541 ggaaatgaaa agtgaagatg ctttatatga tgaagcgtat ttgtttgttg ttgaacaaca
     6601 aaaggcaagt acatcattgt tacaacgcca atttagaatt ggttataata gagcatctag
     6661 gttgatggat gatttagaac gcaatcaggt aatcggtcca caaaaaggaa gcaagcctag
     6721 acaagtttta atagatctta ataatgacga ggtgtaaaaa aatgtcagaa atgaatgcgg
     6781 tatataacgt taaacaatac attttaaatt tgattaagca aaataaattg gaatatggtg
     6841 accaacttcc aagtaattta tcaattgcca gagaattaaa tgtaaaaacc gacgatgttt
     6901 atgaagcaat tcaggcattg attactgaac aagtcattaa agataatttt gaagagggca
     6961 caagtgttaa gtcactgccc cctttctttt atccattgaa tgaacttata agtattgggc
     7021 aaatgattaa aaatgcagga tttgaatgcg gaactgaata cttaaatttt gatgagcaac
     7081 cagcaactat gttagatgca aatttgttga gcgttgaaga aggatatcca gtaaccatta
     7141 tagaacgatt acgaactgcc gatggagaac cggtcgtcta ttgtttagat aaaattgcta
     7201 aaaaagaatt aacatgtaca gagtatcaaa tgagcaatgg atcgattcta agtgcgataa
     7261 aagaacaaag taatcataat atttgttatg ctgatacaga aattgaagcg gtaaattatg
     7321 aacctcgaat atccgaagta ctgaatgctt cgccacacga aggtttgatt ttattaaaaa
     7381 ttacgcacta taatgaatca gatgaaccta ttttgtattc attaaattat atgaaaaata
     7441 gcttagttca atttaaaatc actagaaaaa tatagatgta agaagatata gaggaggcta
     7501 tatatttgag tagacaatct caaccaaata tacatatcaa agtttcacca acaactaaat
     7561 ttaaaacaac tactatagtt tttaaattta tggcaccttt agaatatgac acaataacag
     7621 ctagatcatt attaagcaaa ttattagttc gagcaactaa gaaatggcca accgataagt
     7681 cgtttaataa tcacttagcc gatttatatg gtgcgtatgt gaatagtaca atttcaaaat
     7741 tcaaagatca gcatgtcatt acattttcat tagaaattgt taatgaacgt tatttaagaa
     7801 acggtgaatc attatttaat caaggattag atttattaca agaaatcatt tggaatccat
     7861 taattgaaaa taaagcattc aatgataatt ttgttaacca agagaaaaca ttattagcca
     7921 aaaaaataga agcaatggta gataataaag cacaatattc gtttttaaaa ttactcgacc
     7981 atatgtttga aaatgaagca tataaatact tatctacagg acaactagaa caaatcccac
     8041 atattactgc tgaaacacta tatcatacat atcaatcaat gattaataat gatcaatgtt
     8101 ctgtttatgt tgtcggcaat gtagaacctg aaagtgttga gaaacaaata cgtgaaaaat
     8161 ttgcacttaa accattcgat aaacatcaat tccaacattc tactcatcat ttacacgatg
     8221 aagaagttga ttatattgtt gaatatgatg acgtggatca agctaaatta aatatgggat
     8281 accgttttcc aacacaatat ggacaaagtg gatatgctgc ctttgttgta tttaacatga
     8341 tgtttggagg agatccttca tctgttttat ttaatgaagt gcgagaaaag caaagtttag
     8401 cgtactctat acattcacaa attgatggca aaaatggcta tttatttgtt ttgagtgggg
     8461 tttcaagtga taagtacgaa actgcaaaag acactattat aagtgaattt gaaaaaataa
     8521 aagcaggaga tttcactgaa gaaaaattag agttagctaa aaaagtaatc atttctcatc
     8581 gatatgaatc tgaagatcgt ccgaaaagta ttatagagat tatgcataac caaatattat
     8641 tagagcaacc acaaagcaaa gaaacattta taaatgatat acagaaggta agtcgcgaag
     8701 atattgtttc tgttgctgaa aaagcatttt tagatacaat ctatgtgttg acaaaaggag
     8761 gggataaata atgaaagagc gttattatga attaatagac gaaagagtat tcgaacaaga
     8821 attagaaaat ggtttacgat tatttattat ccccaaacca ggttttcaaa agacatttgt
     8881 cacttacact acacaatttg gttcattaga taatcaattc aaaccccttg gacaagacca
     8941 atttgttact gtacctgatg gagttgctca ctttttagaa cataaattat ttgaaaaaga
     9001 agaagaagac ttatttactg cgtttgctga agataacgca caagcaaatg cgtttacaag
     9061 ctttgatcgt acaagctact tgttcagtgc aactgataat attgaaaaca acattaaacg
     9121 tttacttaca atggttgaaa cgccttattt tacaaaagaa actgttgata aagaaaaagg
     9181 tattattgca gaagaaataa aaatgtatca agagcaacct ggatataaat taatgtttga
     9241 tacattgcgt gcaatgtatc aacaacatcc aatacgtgtt gatattgccg gtagtgtaga
     9301 aagtatatac gatattacaa aagatgattt gtatctatgt tatgaaacgt tttatcatcc
     9361 atcaaatatg gttttatttg ttgttggcga tgtggatcct gaagcaatat gtcgaatagt
     9421 aaaacaacac gaggatgctc gtaataaagt taaccaaccc aaaatcgaac gaggacttgt
     9481 tgatgaaccg gaggatgtta aagaagcatt tgttactgaa tctatgaaaa ttcaatcacc
     9541 aagactaatg cttggtttta aaaataaacc attacaagaa gcgcctcaaa aatatgtaca
     9601 acgtgattta gaaatgtcat tattctttga gttaattttt ggggaagaaa cagattttta
     9661 tcagaattta ttaaacgaag gacttatcga tgatacattt ggttatcaat ttgtactaga
     9721 gccgacgtat agtttttcaa tcgtgacaag tgctactgaa gaaccagata aattaaaaaa
     9781 attattatta gatgagttgc gtgataaaaa aggcaatttc caagatgcag aagcatttga
     9841 acttttgaaa aagcaattta taggtgaatt catatcaagt ttaaactcac ctgaatatat
     9901 tgctaatcaa tatactaaat tgtattttga aggtgttagc gtgtttgaca tgttagacat
     9961 tgttgaaaat atcacgttag atagtattaa cgaaacgtca tcgttatatt taaatttaga
    10021 tcagcaagtc gatagtcgtt tggagattaa aaagtaatga aagcattagt attaggtggt
    10081 tctggttcaa ttggttctga gatagtcaaa caattattaa ctgatggatt tgaagtttat
    10141 gtgcaatatt atcgtactga tataaatgaa ttaactagca aatttaatga tgataaagtt
    10201 cgttttatac aagcggattt atctcaaaca attgatattg acaaaacatt tggtgacatt
    10261 aaatcattag actgtttaat atatgcaagt ggtcagtctt tatatggtgt tttacaagat
    10321 atgaaagacc atgatattga tgcatgttat cagttaaatg tcttgcaatt aattcgatta
    10381 tgtagatatt tcgttgatgt tttacgtcaa agtgccaatg gaagaattat tgtaatttca
    10441 tcaatttggg gtgagacagg agctagtatg gaaactattt attcggcgat gaaaagtgca
    10501 caattaggtt tcgttaaggc gcttagtcaa gagcttgcac taacatcagt gacagtaaat
    10561 gctatcgcac ctggatttgt agccggtaat atggcaagtg agtggcaaga agatgaactt
    10621 caagcaatga taactgaatt accacaacag cgattggttt taccgagtga ggttgctcat
    10681 acatgcgcct atttatatca cccaaatgct agaagtgtta ctggaactat acagaaagtt
    10741 aatggtgctt ggtatattta aaatatagaa ctatttttat gagcaaatgt ttttatgatt
    10801 gatgttataa tatgaatatt acaatgaatc ataatttgtt aattaaacaa ggggtgaggt
    10861 tcaccatgac agttgcagag aaaaaagaat ggtacctaga atacgaaatt gaaattaata
    10921 gaccgggtct tttaggtgat gtatctagtt tattaggtat gttaggtata agtattgtta
    10981 caattaatgg tgttgatcaa ggtaaacgag gccttttaat taaaacagac aatcttgaaa
    11041 aagttgaacg ttttgagcaa atagctcgtg gtataaatga aattgaaata acaaagctta
    11101 aaaaaccaga attaagagac cgtcttgcag taagacatgg aagatatatt gagcaagatg
    11161 caaaagataa gaaaaccttt cgatttgagc gtgaagattt aggcttgtta gtagactttt
    11221 tagctgaatt gttcaaagaa gaaggtcata agttgattgg cattagaggt atgccacggg
    11281 ttggtaaaac tgaatcaatt gttgcgggaa gtgtttgtgc acataagaga tggttattta
    11341 ttagttctac tttaataaaa caaactgtac gtagctctct aattaaaggg gaatatgatg
    11401 ccaatcatgt atacattatt gatggtgcag ttactgccag agaatctaat ccaaaacatc
    11461 aagagcttgt taacgaagtt atgacgttac catcaatcaa agtcgttgaa catccagatt
    11521 tatttgttga aacaagtact tgtacaatgg aagattttga ctatattatc gaattgagag
    11581 aaaatgaaaa tcaagaaata cattacgaag aaatgaagaa acagacagtc caaagtaaga
    11641 ataatttaga ttttggagat ccgtttggtg gtggttttgg tttcttcgag taagtgtaag
    11701 gaggctatga attgaaaacg gtcggtgaag cgctaaaagg tagacgtgaa aggttaggaa
    11761 tgactttaac agaattagag caacgtactg gaattaaacg tgaaatgcta gtgcatattg
    11821 aaaataatga attcgatcaa ctaccgaata aaaattacag cgaaggattt attagaaaat
    11881 atgcaagcgt agtaaatatt gaacctaacc aattaattca agctcatcaa gatgaaattc
    11941 catcgaacca agccgaatgg gacgaagtaa ttacagtttt caataat
//
LOCUS       DAKTIO010000082        11975 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.082, whole genome shotgun sequence.
ACCESSION   DAKTIO010000082 DAKTIO010000000
VERSION     DAKTIO010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11975
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.082"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(29..1120)
                     /locus_tag="O0293_001603"
     CDS             complement(29..1120)
                     /locus_tag="O0293_001603"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HCY0280489.1"
                     /translation="MTTVLFVGLGLIGGSLASNIKYHNPNTNIIAYDADTSQLDKAKS
                     IGIINEKCLNYSEAIKKADVIIYATPVAITNKYLSELIDMPTKPGVIVSDTGSTKAMI
                     QQHECNLLKHNIHLVSGHPMAGSHKSGVLNAKKHLFENAYYILVYNEPRNEQAANTLK
                     ELLSPTLAKFIVTTAEEHDYVTSVVSHLPHIVASSLVHVSQKNGQEHHLVNKLAAGGF
                     RDITRIASSNAQMWKDITLSNKTYILEMIRQLKSQFQDLERLIESNDSEKLLSFFAQA
                     KSYRDALPAKQLGGLNTAYDLYVDIPDESGMISKVTYILSLHNISISNLRILEVREDI
                     YGALKISFKNPTDRERGMQALSDFDCYIQ"
     gene            1285..2316
                     /locus_tag="O0293_001604"
     CDS             1285..2316
                     /locus_tag="O0293_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCY0280490.1"
                     /translation="MEPILEMIKTLTGINSPSGDTEEAIQFVEKYAKDLGYQTTLTNK
                     GALLITVPGKNDEVQRCITAHVDTLGAMVKEIKEDGRLAIELIGGFTYNAIEGEYCQI
                     KTDAGQIYTGTICLHETSVHVYRNNHEIPRDQKHMEIRIDEVTTSEEDTKSLGISVGN
                     FVSFDPRTVITSSGFIKSRHLDDKASVAMILQLLKKLKEEQIILPHTTQFYISNNEEI
                     GYGANASIDSKIKEYIALDMGALGDGQASDEYTVSICAKDASGPYHKQLKSHLVNLCK
                     INNIPYKVDIYPYYGSDASAALHAGADIRHGLFGAGIESSHAMERTHIDSIKATEKLL
                     YAYCLSPIE"
     gene            2808..4214
                     /locus_tag="O0293_001605"
     CDS             2808..4214
                     /locus_tag="O0293_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCY0280491.1"
                     /translation="MDIFYKKIKANVTPEVLAQLHSKKIILESTNQQQTKGRYSVVIF
                     DIYGTLTLDNDVLSVSTLKESYQITERPYHYLTTKINEDYHNIQDEQLKSLPFISGYV
                     GTCSFDLVRHEFPKLQSIQLEDHKQHDVRLYMVEQVYVFDHYKDELYIIATNQFSNST
                     KSDLENRVIKSIEDLTKIQPFMPTQDFDFKTKEIQSNISEERFIEMIQYFKEKITEGD
                     MFQVVPSRIYKYAHHASQHLNQLSFQLYQNLKRQNPSPYMYYLNIDQPYIVGSSPESF
                     VSVKDQIVTTNPIAGTIQRGETTQIDNENMKQLLNDPKECSEHRMLVDLGRNDIHRVS
                     KIGTSKITELMVIEKYEHVMHIVSEVTGKINQNLSPMTVIANLLPTGTVSGAPKLRAI
                     ERIYEQYPHKRGVYSGGVGYINCNHNLDFALAIRTMMIDEQYINVEAGCGVVYDSIPE
                     KELNETKLKAKSLLEVSP"
     gene            4211..4777
                     /locus_tag="O0293_001606"
     CDS             4211..4777
                     /locus_tag="O0293_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HCY0280492.1"
                     /translation="MILVVDNYDSFTYNLVDIVAQHTDVIVQYPDDDNVLNQSVDAVI
                     ISPGPGHPLDDQQLMKIISTYQHKPILGICLGAQALTCYYGGEVIKGDKVMHGKVDTL
                     KVISHHQHLLYQDIPEQFSIMRYHSLISNPDNFPEELKITGRTKDCIQSFEHKERPHY
                     GIQYHPESFATDYGVKIITNFINLVKEG"
     gene            4783..5781
                     /gene="trpD"
                     /locus_tag="O0293_001607"
     CDS             4783..5781
                     /gene="trpD"
                     /locus_tag="O0293_001607"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HCY0280493.1"
                     /translation="MTLLTRIKTETILLESDIKELIDILISPSIGTDIKYELLSSYSE
                     REIQQQELTYIVRSLINTMYPHQPCYEGAMCVCGTGGDKSNSFNISTTVAFVVASAGV
                     KVIKHGNKSITSNSGSTDLLNQMNIQTTTVDDTPNQLNEKDLVFIGATESYPIMKYMQ
                     PVRKMIGKPTILNLVGPLINPYHLTYQMVGVFDPTKLKLVAKTIKDLGRKRAIVLHGA
                     NGMDEATLSGDNLIYELTEDGEIKNYTLNATDYGLKHAPNSDFKGGSPEENLAISLNI
                     LNGKDQSSRRDVVLLNAGLSLYVAEKVDTIAEGIELATTLIDNGEALEKYHQMRGE"
     gene            5783..6565
                     /gene="trpC"
                     /locus_tag="O0293_001608"
     CDS             5783..6565
                     /gene="trpC"
                     /locus_tag="O0293_001608"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HCY0280494.1"
                     /translation="MTILSEIVKYKQSLLQNGYYQDKLNTLKSVKIQNKKSFINAIEK
                     EPKLAIIAEIKSKSPTVNDLPERDLSQQISDYDQYGANAVSILTDEKYFGGSFERLQA
                     LTTKTTLPVLCKDFIIDPLQIDVAKQAGASMILLIVNILSDKQLKDLYNYAISQNLEV
                     LVEVHDRHELERAYKVNAKLIGVNNRDLKRFVTNVEHTNTILENKKTNHYYISESGIH
                     DASDVRKILHSGIDGLLIGEALMRCDNLSEFLPQLKMQKVKS"
     gene            6565..7197
                     /locus_tag="O0293_001609"
     CDS             6565..7197
                     /locus_tag="O0293_001609"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HCY0280495.1"
                     /translation="MKLKFCGFTSIKDVTAASQLPIDAIGFIHYEKSKRHQTITQIKK
                     LASAVPNHIDKVCVMVNPDLTTIEHVLSNTSINTIQLHGTESIDFIQEIKKKYSSIKI
                     TKALAADENIIQNINKYKGFVDLFIIDTPSVSYGGTGQTYDWTILKHIKDIPYLIAGG
                     INSENIQTVNQLKLSHQGYDLASGIEVNGRKDIEKMTAIVNIVKGDRDNE"
     gene            7190..8404
                     /gene="trpB"
                     /locus_tag="O0293_001610"
     CDS             7190..8404
                     /gene="trpB"
                     /locus_tag="O0293_001610"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCY0280496.1"
                     /translation="MNKQIQTEADELGFFGEYGGQYVPETLMPAIIELKKAYKEAKAD
                     PEFQRELEYYLSEYVGRATPLTYAASYTESLGGAKIYLKREDLNHTGAHKINNALGQA
                     LLAKRMGKKKLVAETGAGQHGVASATVAALFDMELVVFMGSEDIKRQQLNVFRMELLG
                     AKVVAVEDGQGTLSDAVNKALQYWVSHVDDTHYLLGSALGPDPFPTIVRDFQSVIGKE
                     IKSQILKKEGRLPDAIVACIGGGSNAIGTFYPFIKDDVALYGVEAAGQGDDTDKHALA
                     IGKGSPGVLHGTKMYLIQDEDGQVQLAHSISAGLDYPGIGPEHSYYHDIGRVTFENAS
                     DTQAMNALINFTKHEGIIPAIESAHALSYVERLAPTMSKEDIIVVTISGRGDKDMETI
                     RQYMVERGLAND"
     gene            8397..9125
                     /locus_tag="O0293_001611"
     CDS             8397..9125
                     /locus_tag="O0293_001611"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCY0280497.1"
                     /translation="MTKLFIPYIMGNKDLIENATLLSENGADIIEIGVPFSDPVADGP
                     VIMEAGQQAIKQGITIDYIFNQLEKHGDQIKCNYVLMTYYNIICHYGEQAFFEKCRDT
                     GVYGLIIPDLPYELSQRLKQQFSHYGVKIISLVAMTTDDKRIKDIVSHAEGFIYTVTM
                     NATTGQNGAFHPELKRKIESIKAIANVPVVAGFGIRTPQHVADIKEVADGIVIGSEIV
                     KRFKSNTREEIIKYLQSIQQTLNN"
     gene            9447..10709
                     /gene="femA"
                     /locus_tag="O0293_001612"
     CDS             9447..10709
                     /gene="femA"
                     /locus_tag="O0293_001612"
                     /EC_number="2.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemA"
                     /protein_id="HCY0280498.1"
                     /translation="MKFTNLTAKEFGAFTDSMPYSHFTQTVGHYELKLAEGYETHLVG
                     IKNNNNEVIAACLLTAVPVMKVFKYFYSNRGPVIDYENQELVHFFFNELSKYVKKHRC
                     LYLHIDPYLPYQYLNHDGEITGNAGNDWFFDKMSNLGFEHTGFHKGFDPVLQIRYHSV
                     LDLKDKTADDIIKNMDGLRKRNTKKVKKNGVKVRFLSEEELPIFRSFMEDTSESKAFA
                     DRDDKFYYNRLKYYKDRVLVPLAYINFDEYIKELNEERDILNKDLNKALKDIEKRPEN
                     KKAHNKRDNLQQQLDANEQKIEEGKRLQEEHGNELPISAGFFFINPFEVVYYAGGTSN
                     AFRHFAGSYAVQWEMINYALNHGIDRYNFYGVSGKFTEDAEDAGVVKFKKGYNAEIIE
                     YVGDFIKPINKPVYAAYTALKKVKDRIF"
     gene            10728..>11975
                     /gene="femB"
                     /locus_tag="O0293_001613"
     CDS             10728..>11975
                     /gene="femB"
                     /locus_tag="O0293_001613"
                     /EC_number="2.3.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemB"
                     /protein_id="HCY0280499.1"
                     /translation="MKFTELTVTEFDNFVQNPSLESHYFQVKENIVTRENDGFEVVLL
                     GIKDDNNKVIAASLFSKIPTMGSYVYYSNRGPVMDFSDLGLVDYYLKELDKYLQQHQC
                     LYVKLDPYWLYHLYDKDIVPFEGREKNDALVNLFKSHGYEHHGFTTEYDTSSQVRWMG
                     VLNLEGKTPETLKKTFDSQRKRNINKAINYGVKVRFLERDEFNLFLDLYRETEERAGF
                     VSKTDDYFYNFIDTYGDKVLVPLAYIDLDEYVLKLQQELNDKENRRDQMMAKENKSDK
                     QMKKIAELDKQIDHDQHELLNASELSKTDGPILNLASGVYFANAYEVNYFSGGSSEKY
                     NQFMGPYMMHWFMINYCFDNGYDRYNFYGLSGDFTENSEDYGVYRFKRGFNVQIEELI
                     GDFYKPIHKVKYWLFTTLDKLRKK"
BASE COUNT         4380 a         1746 c         2203 g         3646 t
ORIGIN      
        1 atggaattta tcaaaagaga cttaataatt attggatata acaatcaaaa tcactcaatg
       61 cttgcatacc gcgttctcgg tcagtagggt ttttgaaact aatttttaaa gcaccgtata
      121 tatcttcgcg tacttctaag attcttaagt tgcttataga tatgttatgt aaactcagga
      181 tataagtcac tttacttatc atacctgatt catccggaat gtctacatat agatcatacg
      241 cagtatttag tccacctagt tgtttagcgg gtagtgcgtc gcgatacgat ttagcttggg
      301 caaaaaatga taacaatttt tcagaatcat tgctttcaat tagtctttct aaatcttgaa
      361 actgactttt tagctgtcga atcatttcta aaatatacgt tttattactc aaggtgatat
      421 ctttccacat ttgtgcatta ctactagcta tacgagtgat atcacgaaaa ccaccagctg
      481 caagtttatt aactaaatga tgttcttgac cgttcttttg actaacatga actaaactag
      541 atgcaacgat atgaggtaaa tgacttacga cgcttgttac gtagtcgtgt tcttcagcag
      601 tagttacaat aaatttagca agagtaggtg ataacagttc ttttaacgtg tttgctgctt
      661 gctcatttct tggctcattg tagactaaaa tataataagc gttttcaaat aagtgctttt
      721 tagcatttag tacaccagat ttatgactac cagccattgg atgaccactg actaaatgaa
      781 tattatgctt taataaattg cattcgtgtt gctgtatcat tgctttagta ctaccagtat
      841 cagaaacaat aacaccaggt ttagttggca tatctataag ctcgctaaga tatttatttg
      901 tgatagcaac aggtgttgca taaataatta catcggcttt tttaatagct tcactataat
      961 ttaaacattt ttcattaata atgccgattg atttagcttt atctaactga gaagtatctg
     1021 catcgtatgc aataatatta gtattagggt tatggtattt tatattgcta gcaagacttc
     1081 caccaattaa tccaagccca acaaataaaa ctgttgtcat ataaatcacc ttatttcgaa
     1141 attttcagaa taataacatt gtaaatgagc tgttgacaca gtgcaatagt aaataaaaat
     1201 cgataatagc attaatagat aaacggagat aaatcatcta caataaagag tatagtaaca
     1261 caatggcaac ggaggggtaa atcaatggaa ccaatattag aaatgattaa aacattaaca
     1321 ggtattaata gtccttcagg agacacagaa gaagcaattc aatttgtcga aaaatatgca
     1381 aaagacttgg gttatcaaac aacactaaca aataaaggtg cgttattaat aacagtgcca
     1441 ggcaaaaatg atgaagtaca acgctgtatt actgctcatg ttgatacttt aggtgcaatg
     1501 gttaaagaaa ttaaagaaga tggtcgctta gcaatagaat taattggagg attcacgtat
     1561 aacgcgattg agggtgaata ttgccaaatt aaaactgatg ctggtcaaat atatacagga
     1621 acaatttgtc tgcatgaaac aagtgttcat gtatatagaa ataatcatga aatacctaga
     1681 gatcaaaagc atatggaaat aagaattgat gaagtaacta catcagaaga agatacaaag
     1741 agtttaggta tttcagtagg taattttgtt agctttgatc cacgtacagt tatcacgtca
     1801 tcaggtttta ttaaatctcg tcatttagat gataaagcta gcgtagcgat gatactacaa
     1861 ttactaaaga aattaaaaga agagcaaata atattaccac atacaacgca attttatatt
     1921 tctaataacg aagaaatagg ttacggtgca aatgcatcaa ttgattcgaa aatcaaagaa
     1981 tatattgcat tagatatggg cgcgttggga gacggtcaag catcggatga atatacagtt
     2041 tctatttgtg ccaaagatgc ttcaggtcca tatcataagc aattgaaatc gcacctagtt
     2101 aatctttgca aaataaataa cattccatat aaagtagaca tatatccata ttatggttca
     2161 gatgcttcag cagctttaca tgctggtgcg gatatcagac atggtttatt tggcgctggc
     2221 attgaatcat ctcatgcaat ggaacgaaca catattgatt ctattaaagc gacagagaaa
     2281 ttactatatg catattgctt atcaccaatt gagtaaacaa ttagtgttga caaatgtgaa
     2341 cgacctatgt aatataatga actataaaaa taattagaat tttctaaaga aatagtagca
     2401 gatatgaaac gtagcaaata gaaagctaat gggtgatggg aattagcacg ccatatcttg
     2461 tgaattggac tttggaaaac aattgaatga gttttgaaag tgaacatgaa ttatgttaac
     2521 taaggtggca ccacggtaac gcgtccttac aggtatatgc gttatgtggt gtctttttat
     2581 ttagacaaaa tgtagtagtt aattaaaggt agcaacagaa agttagtgga tgatgtgaac
     2641 taacaccgag attaatgaaa ttgggttttg tctgcaacag aaaaattata tatagtaaag
     2701 agtgaactat gaatatttcg aatattcggt taatttaggt ggtaccacgc gtcagcgtcc
     2761 tttatattga taaggatgct ggcgcttttt tgaaaggagc gtatagaatg gatatatttt
     2821 ataaaaaaat aaaagcaaat gtaacgcccg aagttttagc acaacttcat tccaagaaga
     2881 tcattttgga aagtacaaat caacaacaaa ctaaaggtcg ctattcagtt gttatttttg
     2941 atatttatgg cactttaact ttagataatg atgtattatc agtaagtact ttaaaagaat
     3001 cgtatcaaat cactgaaaga ccgtaccatt atttaacgac taaaataaat gaagactacc
     3061 ataatattca agatgagcaa cttaagtcat taccatttat atctggatat gttgggacgt
     3121 gtagctttga tttagtaaga catgaatttc ctaaattgca atcaatacaa ttagaagatc
     3181 acaagcagca cgatgtaagg ttatatatgg ttgaacaagt ttatgtattt gaccattaca
     3241 aagatgagtt atatatcatc gcgacgaatc aattttcaaa ttcaacaaaa tcagatcttg
     3301 agaatcgagt tattaagtct atcgaagact taactaaaat ccaaccattc atgcctacac
     3361 aagattttga ttttaaaact aaagaaattc aatcaaacat ttctgaagaa agatttatcg
     3421 aaatgattca gtatttcaaa gagaaaataa cagaagggga tatgttccaa gttgtgccat
     3481 caagaattta caaatatgcg catcatgcta gtcagcattt aaatcaactt tcgtttcaac
     3541 tgtatcaaaa tttaaaacga caaaacccaa gtccatatat gtattatctt aatatcgatc
     3601 aaccatatat tgtcggtagt tctcccgaaa gtttcgtaag tgtcaaagat caaattgtaa
     3661 caactaatcc tattgcaggt acgattcaac gtggtgagac gacacaaata gataatgaga
     3721 atatgaaaca actacttaat gatccaaaag aatgcagcga acatcgtatg ctagttgatt
     3781 taggacgtaa tgatattcat agagtaagta aaatcggtac ctcaaaaatt actgaattaa
     3841 tggttattga aaaatatgaa catgttatgc atatcgtaag tgaagtcaca ggtaaaataa
     3901 atcaaaattt atcgccaatg acagttattg cgaatttatt accaacaggt accgtttcag
     3961 gtgcaccaaa attacgtgca attgaaagaa tatatgaaca atatccacat aaacggggcg
     4021 tttatagtgg tggtgttgga tacataaatt gtaatcataa cttagatttt gcattagcaa
     4081 ttcgaacgat gatgatagat gagcagtata tcaacgtaga agctggttgt ggcgttgtat
     4141 atgattctat tcctgaaaaa gaactgaatg aaacgaaatt gaaagctaaa agcttattgg
     4201 aggtgagccc atgatcttag ttgtagataa ttatgattcc tttacatata acctagtgga
     4261 tattgttgct caacatactg acgtcattgt tcaataccct gatgatgata atgtgctgaa
     4321 tcaatcggtg gacgctgtta ttatatctcc tggtccaggg catccattag acgatcaaca
     4381 gttaatgaaa atcatatcaa cctatcaaca caaacccatt ttaggtattt gtttaggggc
     4441 tcaggcactg acttgttact acggtggaga agtcattaaa ggcgacaagg ttatgcacgg
     4501 caaagttgat acactaaagg ttatatcgca tcatcaacat ctgttatatc aagatatacc
     4561 agaacagttt tcaattatga gatatcattc attaataagt aaccctgaca attttccaga
     4621 agaattgaaa attactggac gtaccaaaga ttgtatacag tcattcgagc ataaagaaag
     4681 accgcattat ggtattcagt accatcctga atcatttgct acagactatg gtgtcaaaat
     4741 aattacaaat ttcattaatc tagtgaagga aggatgaaaa ccatgacatt actaacaaga
     4801 ataaaaactg aaactatatt acttgaaagc gacattaaag agctaatcga tatacttatt
     4861 tctcctagta ttggaactga tattaaatat gaattactta gttcctattc ggagcgagaa
     4921 atccaacaac aagaattaac atatattgta cgtagcttaa ttaatacaat gtatccacat
     4981 caaccatgtt atgaaggggc tatgtgtgtg tgcggcacag gtggtgacaa gtcaaatagt
     5041 ttcaacattt caacgactgt tgcttttgtt gtagcaagtg ctggcgtaaa agttataaaa
     5101 catggtaata aaagtattac ctcaaattca ggtagtacgg atttgttaaa tcaaatgaac
     5161 atacaaacaa caactgttga tgatacacct aaccaattaa atgaaaaaga ccttgtattc
     5221 attggtgcaa ctgaatcata tccaatcatg aagtatatgc aaccagttag aaaaatgatt
     5281 ggaaagccta caatattaaa ccttgtgggt ccattaatta atccatatca cttaacgtat
     5341 caaatggtag gcgtctttga tcctacaaag ttaaagttag ttgctaaaac gattaaagat
     5401 ttaggtagaa aacgtgcaat cgttttacat ggtgcaaatg gtatggatga agcaacacta
     5461 tctggtgata atttgatata tgaattgact gaagatggag aaatcaaaaa ttacacatta
     5521 aatgcgactg attatggttt gaaacatgcg ccgaatagtg attttaaagg cggttcacct
     5581 gaagaaaatt tagcaatctc ccttaatatc ttgaatggta aagatcagtc aagtcgacgt
     5641 gatgttgtct tactaaatgc gggtttaagc ctttatgttg cagagaaagt ggataccatc
     5701 gcagaaggca tagaacttgc aactacattg attgataatg gtgaagcatt ggaaaaatac
     5761 catcaaatga gaggtgaata atatgacgat tttatcagaa attgttaaat ataaacagtc
     5821 acttttacaa aatggctatt atcaagacaa acttaatacc ttgaaaagtg tgaagattca
     5881 gaataaaaaa tcttttataa acgcaattga gaaagaacca aagctagcaa ttattgcaga
     5941 aattaaatcg aagagtccta cagttaatga cttacctgaa cgagatttat cgcaacaaat
     6001 ctcagattat gaccaatatg gtgcaaatgc cgtgtccatt ttaactgatg aaaagtactt
     6061 tggtggtagt tttgaaagat tacaagcatt gacgacaaaa acaacattac ccgtattatg
     6121 caaagacttt attatagacc cgcttcaaat tgatgttgct aaacaagctg gtgcatctat
     6181 gattttattg atcgttaaca tcttatctga taaacaattg aaagatttat ataactacgc
     6241 tatatcgcaa aatctagaag tgttagttga agtacatgat cgccatgaat tagaacgtgc
     6301 ctataaggtt aatgctaaat tgattggtgt aaataacagg gacttaaaac gatttgttac
     6361 aaatgtggaa catacaaata ctattttaga aaataaaaaa acaaatcatt attatatttc
     6421 tgaaagtggt attcacgatg catctgatgt aagaaaaatc ttgcatagtg gtatcgatgg
     6481 cttactaata ggtgaggcgc ttatgcgttg tgacaatcta tctgaatttt taccacaact
     6541 gaaaatgcaa aaggtgaagt catgatgaaa ttgaaatttt gtggctttac atcaataaag
     6601 gatgttacag cggccagtca attacctatt gatgcgatag gtttcatcca ttatgaaaaa
     6661 agtaaaaggc atcaaacaat tacccaaata aaaaagttag cgtctgctgt tccaaatcat
     6721 atcgataaag tatgtgtcat ggtaaatcct gatttaacaa caattgaaca cgtattaagc
     6781 aatacgtcaa ttaacacaat acagttacac ggcacagaat ctattgattt tatacaggaa
     6841 attaaaaaga aatattcaag cattaaaatc actaaagctt tagctgcaga tgaaaacata
     6901 atccaaaaca taaataaata taaagggttc gtagatttat ttattatcga cacaccctca
     6961 gtgtcgtatg gtggtaccgg tcaaacatat gactggacta ttttgaagca cataaaagac
     7021 ataccttatt tgatagcagg aggcattaac tctgaaaata ttcaaacagt taatcaactt
     7081 aaattatcac atcaaggtta tgatcttgca tcaggtatag aagtaaatgg gcgaaaagat
     7141 atagaaaaaa tgacagcaat tgtaaatatt gtgaaaggag atagagacaa tgaataaaca
     7201 aatacaaaca gaagcagatg aattaggttt ctttggtgaa tatggagggc aatatgttcc
     7261 agaaacatta atgccagcaa ttattgagtt gaaaaaagct tataaagagg caaaagcaga
     7321 cccagagttt caaagagaac tggaatacta tttatcagag tatgtaggac gcgcgacacc
     7381 acttacatat gctgcatcat atactgaaag cctaggtggc gctaaaatat atttgaaacg
     7441 agaggatcta aatcatacag gcgcccataa aattaataat gcgttaggtc aagcgttgct
     7501 tgctaaaaga atgggcaaga agaagcttgt tgctgaaact ggtgcgggtc aacatggtgt
     7561 agctagtgct acggttgctg cattatttga tatggaactt gttgtcttta tgggaagtga
     7621 agatattaaa agacaacaac ttaatgtatt tagaatggaa ttacttggtg caaaggttgt
     7681 ggcagttgaa gatggtcaag ggactttatc ggatgcagtt aataaagcat tgcaatattg
     7741 ggtaagtcat gtagatgata cacattattt attaggttct gcattaggtc cagacccgtt
     7801 cccaacgatt gttagagatt ttcagagtgt gattggtaaa gaaataaaat cacagatatt
     7861 gaagaaagaa ggtcgacttc cggatgcaat tgtagcatgt atcggtggtg gctcaaatgc
     7921 aatcggtaca ttttatccat ttattaaaga tgatgttgca ttatacggtg ttgaagccgc
     7981 aggtcaaggc gatgatactg ataaacatgc acttgcaatt ggcaaaggat cacctggcgt
     8041 attacatggt actaaaatgt atttaattca agatgaagat gggcaagtgc aactagcaca
     8101 ttctatttca gcaggacttg attatcctgg tattggacca gaacattctt attaccacga
     8161 cattggtaga gtaacttttg aaaatgctag tgatacacaa gcaatgaatg ctttaatcaa
     8221 ctttacaaaa catgaaggta ttatacctgc aattgaaagt gcacatgcac tgagttatgt
     8281 tgaaagacta gcgcctacga tgtcgaaaga agatattatt gtagtaacta tttctggacg
     8341 tggcgataaa gatatggaaa caattagaca atatatggta gagcgaggtc ttgcaaatga
     8401 ctaaattatt tataccttat attatgggca ataaagattt gattgaaaat gcaacattgt
     8461 tgagtgaaaa tggtgcagat ataattgaaa ttggagtacc tttctctgat ccggttgctg
     8521 atggtccagt tatcatggaa gcaggtcaac aagcgattaa acaaggcatc acgatagatt
     8581 atattttcaa tcaattagaa aaacatggtg atcaaattaa gtgtaactat gtattaatga
     8641 cgtattataa tattatttgt cattatggag aacaagcgtt ttttgaaaaa tgtcgagata
     8701 ctggtgtcta cggcttaatt attcctgatt taccatatga attatcgcag cgtttaaaac
     8761 aacaatttag tcactatggc gtcaaaatca tatcgttagt tgcgatgact actgatgaca
     8821 aacgtataaa agatatcgta tcccatgcgg aaggctttat ttatactgtg acgatgaatg
     8881 cgacaacagg gcaaaacggt gcgtttcatc cagaattaaa acgaaaaatt gagtcaatta
     8941 aagcgatagc caatgtgcca gttgtcgcag gatttggtat aagaacacca caacatgttg
     9001 cagatataaa agaggttgca gatggcattg tcattggtag cgaaatcgtt aagcgattta
     9061 aatctaacac gcgtgaggaa atcattaaat atttacaatc tatccaacaa acattgaata
     9121 attaagttta cttgatttaa aaaaattagg cgaatactgt ttgaaaaagt gaaaaacggt
     9181 gaattataaa attgaataga aatgatacaa aagtaatatg agcaaaccca aacgttcata
     9241 ttactttttt tgaaattgta ttcaaaaatc taaatattac tataaaagta tacgcaatta
     9301 aagcgtttat gttttagttt taacattaac tattgtatac ttatttagat tagatttatt
     9361 atttttgaca tttgcagagg ggaaatagaa aaactgcaaa tacggaaatg aaattaatta
     9421 acgagagaca aataggagta atgataatga agtttacaaa tttaacagct aaagagtttg
     9481 gtgcctttac agatagcatg ccatacagtc atttcacgca aactgttggc cactatgagt
     9541 taaagcttgc tgaaggttat gaaacacatt tagtgggaat aaaaaacaat aataacgagg
     9601 tcattgcagc ttgcttactt actgctgtac ctgttatgaa agtgttcaag tatttttatt
     9661 caaatcgcgg tccagtgatt gattatgaaa atcaagaact cgtacacttt ttctttaatg
     9721 aattatcaaa atatgttaaa aaacatcgtt gtctatacct acatatcgat ccatatttac
     9781 catatcaata cttgaatcat gatggcgaga ttacaggtaa tgctggtaat gattggttct
     9841 ttgataaaat gagtaactta ggatttgaac atactggatt ccataaagga tttgatcctg
     9901 tgctacaaat tcgttatcac tcagtgttag atttaaaaga taaaacagca gatgacatca
     9961 ttaaaaatat ggatggactt agaaaaagaa acacgaaaaa agttaaaaag aatggtgtta
    10021 aagtaagatt tttatctgaa gaagaactac caatttttag atcatttatg gaagatacgt
    10081 cagaatcaaa agcttttgct gatcgtgatg acaaatttta ctacaatcgc ttaaaatatt
    10141 acaaagaccg tgtgttagta cctttagcgt atatcaactt tgatgaatat attaaagaac
    10201 taaacgaaga gcgtgatatt ttaaataaag atttaaataa agcgttaaag gatattgaaa
    10261 aacgtcctga aaataaaaaa gcacataaca agcgagataa cttacaacaa caacttgatg
    10321 caaatgagca aaagattgaa gaaggtaaac gtctacaaga agaacatggt aatgaattac
    10381 ctatctctgc tggtttcttc tttatcaatc catttgaagt tgtttattat gctggtggta
    10441 catcaaatgc attccgtcat tttgccggaa gttatgcagt gcaatgggaa atgattaatt
    10501 atgcattaaa tcatggcatt gaccgttata atttctatgg tgttagtggt aaatttacag
    10561 aagatgctga agatgctggt gtagttaaat tcaaaaaagg ttacaatgct gaaattattg
    10621 aatatgttgg tgactttatt aaaccaatta ataaacctgt ttacgcagca tataccgcac
    10681 ttaaaaaagt taaagacaga attttttagg aagggaatta tcaaaacatg aaatttacag
    10741 agttaactgt taccgaattt gacaactttg tacaaaatcc atcattggaa agtcattatt
    10801 tccaagtaaa agaaaatata gttacccgtg agaatgatgg ctttgaagta gttttattag
    10861 gtattaaaga cgacaataac aaagtaattg cagcaagcct tttctctaaa attcctacta
    10921 tgggaagtta tgtttactat tcgaatcgtg gtccagtaat ggatttttca gatttaggat
    10981 tagttgatta ttatttaaaa gagttagata aatatttaca gcaacatcaa tgtttatatg
    11041 ttaaattaga tccgtattgg ttatatcatc tatatgataa agatatcgtg ccatttgaag
    11101 gtcgcgagaa aaatgatgcc ctagtaaact tgtttaaatc acatggttac gagcatcatg
    11161 gctttacaac tgagtatgat acatcgagcc aagtacgatg gatgggcgta ttaaaccttg
    11221 aaggtaaaac acccgaaaca ttgaaaaaga catttgatag tcaacgtaaa cgtaatatta
    11281 ataaagcgat aaactatggt gttaaagtca gattccttga acgtgatgag ttcaatcttt
    11341 tcttagattt atatcgtgaa actgaagagc gtgctggatt tgtatcaaaa acagatgatt
    11401 atttttataa ctttattgac acatatggag ataaagtatt agtaccatta gcatatattg
    11461 accttgatga atatgtgtta aagttgcaac aggaattgaa tgacaaagaa aatcgtcgtg
    11521 atcaaatgat ggcgaaagaa aacaaatcag ataagcaaat gaagaaaatt gcagaattag
    11581 ataagcaaat tgatcatgat cagcatgaat tattgaatgc aagtgaattg agcaaaacgg
    11641 acggcccaat tctaaacctt gcttctggcg tttattttgc aaatgcatat gaagtgaatt
    11701 atttctctgg tggttcatca gaaaaatata atcaatttat gggaccatac atgatgcatt
    11761 ggtttatgat taactattgc ttcgataatg gctatgatcg ttataatttc tatggtttat
    11821 caggtgattt tacggaaaac agtgaagatt atggcgtata ccgctttaaa cgtggattta
    11881 atgtacaaat cgaagaatta ataggggatt tctataaacc aattcataaa gtgaaatatt
    11941 ggttgttcac aacattggat aaattacgta aaaaa
//
LOCUS       DAKTIO010000083        11851 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.083, whole genome shotgun sequence.
ACCESSION   DAKTIO010000083 DAKTIO010000000
VERSION     DAKTIO010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11851
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.083"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            194..514
                     /locus_tag="O0293_001614"
     CDS             194..514
                     /locus_tag="O0293_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HCY0280500.1"
                     /translation="MPHILNVTVPIPETHVLITKDEYDELIGYSLDPVWNMSDLKKKL
                     KIASDETIKDRLLFHPRFEKELRAQGIVHYPDESFNRWRFNARKMNKFVDEHFNEIYK
                     ERIK"
     gene            511..672
                     /locus_tag="O0293_001615"
     CDS             511..672
                     /locus_tag="O0293_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
                     /protein_id="HCY0280501.1"
                     /translation="MSDTYKSYLLAVLCFTVLAIVLMPLLYFTTAWSIAGFASIGTFI
                     YYKEYFYEE"
     gene            767..1027
                     /locus_tag="O0293_001616"
     CDS             767..1027
                     /locus_tag="O0293_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001794769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2482 family protein"
                     /protein_id="HCY0280502.1"
                     /translation="MTKNYKDMTQDELRGLLAEKNAELFDLASEIDEETEFDVLLFSN
                     VGISNGDFTPSSHCVIGNVVDIANLLKRRAVYRDIADVIKMR"
     gene            1067..1327
                     /locus_tag="O0293_001617"
     CDS             1067..1327
                     /locus_tag="O0293_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HCY0280503.1"
                     /translation="MYYKTGDVCRKIINVDGFDFQLRVKKRAYSVEMVVLDHEGNSID
                     GLLVSDENDLYTALDILKQSVYEWIENNTDEQDKLMNLVMKW"
     gene            1336..1599
                     /locus_tag="O0293_001618"
     CDS             1336..1599
                     /locus_tag="O0293_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001638663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280504.1"
                     /translation="MRDTERNILNIFKTLFDEYTLSNQRALLEIERNHHGYLSINFLH
                     YHDSYKTNNKLVQIHEINPDSHERIKNLIIEVLRGHRKIKKGA"
     gene            1608..3551
                     /locus_tag="O0293_001619"
     CDS             1608..3551
                     /locus_tag="O0293_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCY0280505.1"
                     /translation="MKINKLTISNFAGIKEEKFNFDGKDAKIYGNNATGKTTTATALQ
                     WLLFDKGLDGSTKSFNPVPLNEKNEENYELIPTVFAEFEIDGKITTFKKESHPKYTIN
                     QKTNRKEYSRSRTKKQYINDESIKVKDYKARIDELIDEDVFKLITNPQAFNLLDWKKR
                     RSLLFEIAKPINDEDVIKTNDDFKELNNILGDHEIETKKKILTDKIKQINKDIKDIPI
                     RINQTQQNKQDVPEFDNDRYAIIKQEIEQLENERIDIQNGKEEINLRNQLADKQSELK
                     RIEDNNSASNENKIHALTNELHVENGTVANLKTRLKQNKQQITHEENRRNQLLENHKG
                     LKSDLEKAKNQKFEYLDDNVCSCCGQKLPVEQVDMAREKALQKFNASKSKELETIQTS
                     INHIISEGKKIKLIIEKLEDDNNNLQIKINEAEERSARIQNKINKLKTTHVDVTQTDE
                     YKAVMLEINEINQKRSNIRKTIQDKVSGIDDKISELTQEKSEIEVSRSIEKSNKHLDD
                     VISELRNEEDRLLDEKEKYSHDLYILKEFTTTKVKMLTENINNEFEIAEFKLFNTLVN
                     GELEETCSTTVNGVEYDSGLNNASRINVGLDIINTLSKHFKVTAPIFIDNAESVTELI
                     KTESQQIQLIVNEQDKKLRMETI"
     gene            3553..4473
                     /locus_tag="O0293_001620"
     CDS             3553..4473
                     /locus_tag="O0293_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HCY0280506.1"
                     /translation="MTENNKLQTIEQQLVQEKNVSDNVLNKVRVLESQGNLELPNDYS
                     PSNAMKQAWLQISQDNKLMSCNDTSKANALLDMVTQGLNPAKNQCYFIPYGNKMQLQR
                     SYHGNVMMLKRDAGAQDVVAQVIYKGDTFKQEMGETGRIKAIKHEQDFFNIDKENIIG
                     AYCTIVFNDGRDNYIEVMTIEQIKQAWMQSSMIKDEKALQNSKTHNNFKEEMAKKTVI
                     NRAAKRYINTSTDSNLFKYAQESEQRQRKEVLDAEVEENANQEQLDFEQPVLEEAQYT
                     ELENDKPIDVSDFEEIKEPATEKESEEEPF"
     gene            4554..5171
                     /locus_tag="O0293_001621"
     CDS             4554..5171
                     /locus_tag="O0293_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCY0280507.1"
                     /translation="MKFERVQKHFKYKTRHIAGCLITHEHGDHAKYTKQFVDNGVISY
                     MTAGTQQAMNFESHRLCTIKAKQELRIGTWSILPFDIEHDANEPVAFLLQSTLGYKVL
                     YVTDTKYLKYKFNGITHMMLEVNYIYEQMQENIKNGSVHSTLANRIMESHFSLEHAIG
                     MLKANDLTRLEEIHLIHLSSQNSNAKYIKSEIQKVTGAPVYFGGL"
     gene            5172..5642
                     /gene="ssb"
                     /locus_tag="O0293_001622"
     CDS             5172..5642
                     /gene="ssb"
                     /locus_tag="O0293_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCY0280508.1"
                     /translation="MLNRTILVGRLTRDPELRTTQNGVNVASFTLAVNRTFTNAQGER
                     EADFINIIVFKKQAENVNKYLSKGSLAGVDGRLQTRNYENKEGQRVYVTEVIADSIQF
                     LEPKNSNDTQQDLYQQQVQQTRGQSQYSNNKPVKDNPFANANGPIELNDDDLPF"
     gene            5672..6565
                     /locus_tag="O0293_001623"
     CDS             5672..6565
                     /locus_tag="O0293_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HCY0280509.1"
                     /translation="MTGWISIDRSIQNHWLFKEKRTFSKFEAWIYLLMEANHSKAKVP
                     IGNQIVTVERGQRLTSILTLSDLFNWSRFKVKTFLDLLESDGMLEVKTTSKYTLITIV
                     NYDFYQSEQGRNQHQNDIKPTSKQHQSNINPTSKQHQTNTNNNDNKDNNEKNVNNEKK
                     KTTAFDFFQDNGFGFITSYNLDDLNYYLDSFENDSDEIVTASLKIAKDRNKVTWGYAK
                     SILNTWLNANLKSIEQVRAFEKQQLESKKQNYKPFVKQSKEKTPKWLTDSTRETKTPE
                     VDENLEKDREAFIKRLNSKWE"
     gene            6572..6790
                     /locus_tag="O0293_001624"
     CDS             6572..6790
                     /locus_tag="O0293_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280510.1"
                     /translation="MDAFDKYYLFDHDGNKMFSVTPHFKDGRHLVVGLKHTKFNGRRW
                     YLDDYELKTLIDNEQMELGHQTSLFEYI"
     gene            6799..7203
                     /locus_tag="O0293_001625"
     CDS             6799..7203
                     /locus_tag="O0293_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCY0280511.1"
                     /translation="MEIEIKFNEVFNAPMGSPRPRFSTKGRYAHTYMPTKYTEHKKYL
                     QNQMPKLNLENALKIELEFYFTPPKSWSKKKKTQAIGQLKVTKPDIDNLMKTVLDACN
                     NYLWKDDNQITEITSSKRYGIEPKIIIRIEEI"
     gene            7216..7584
                     /locus_tag="O0293_001626"
     CDS             7216..7584
                     /locus_tag="O0293_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280512.1"
                     /translation="MARKARIVTINDKPYRFTKSEMELIESHGITAGMVSKRVKDGWE
                     LHEAMDAPEGTRLSEYREKKTIERLEQARLERKLERKRKKEAELRRKKPHLFNVPQKH
                     PRGRYACYLMENDIFVKVKK"
     gene            7588..7830
                     /locus_tag="O0293_001627"
     CDS             7588..7830
                     /locus_tag="O0293_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phi PVL orf 51-like protein"
                     /protein_id="HCY0280513.1"
                     /translation="MTDNARKEYLNQFFGSKRYLYQDNERVAHIHVVNGTYYFHGHIV
                     PGWQGVKKTFDTAEELETYIKQQGLEYEEQKQLTLF"
     gene            7839..8210
                     /locus_tag="O0293_001628"
     CDS             7839..8210
                     /locus_tag="O0293_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCY0280514.1"
                     /translation="MKVNYETGFQIGVMEARLKKMRKQRDEYKKQRDELIGDIAKLRE
                     RNEELENMWRTLKNELFGRYEFYRFRLSELQIESRANKEVAIYRRAEINLSVILCRMD
                     KLDGTNEFYEFLGQMEDDTNE"
     gene            8203..8439
                     /locus_tag="O0293_001629"
     CDS             8203..8439
                     /locus_tag="O0293_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HCY0280515.1"
                     /translation="MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEIRE
                     AINAQWVEYPEDWASEVLREVRELEYEEEQENEN"
     gene            8429..8671
                     /locus_tag="O0293_001630"
     CDS             8429..8671
                     /locus_tag="O0293_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280516.1"
                     /translation="MKINESLKKLKEKGYKENEDKAIFNLADGTLEIYIDHDEKTIIT
                     EFHDLKVFVSEDLKDKSMESVMYELAGIDEEDKEND"
     gene            8664..9197
                     /locus_tag="O0293_001631"
     CDS             8664..9197
                     /locus_tag="O0293_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP pyrophosphatase"
                     /protein_id="HCY0280517.1"
                     /translation="MTNILQVKLLSENARMPERNHKTDAGYDIFSAETVVLEPQEKAV
                     IKTDVAVSIPEGYVGLLTSRSGVSSKTHLVIETGKIDAGYHGNLGINIKNDNETLESE
                     DMSNFGRSPAGIDGKYARLPVTDKILCMNGSYVINKGDKLAQLVIVPIWTPELKQVEE
                     FESVSERGAKGFGSSGV"
     gene            9234..9479
                     /locus_tag="O0293_001632"
     CDS             9234..9479
                     /locus_tag="O0293_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280518.1"
                     /translation="MSDMLEIFLIGFGVYLFYRIAIIFLKSKKTIHTNIYEMLMLATI
                     FMISTFAYKHQKTHILIAFLVMFFMSKLKQVQGSYEE"
     gene            9476..9682
                     /locus_tag="O0293_001633"
     CDS             9476..9682
                     /locus_tag="O0293_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HCY0280519.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAKEKY
                     EKQVKRDAVIKVGQLFENIRECGK"
     gene            9679..10065
                     /locus_tag="O0293_001634"
     CDS             9679..10065
                     /locus_tag="O0293_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280520.1"
                     /translation="MIKKLKNMDGFDIFIVGILSLFGITALLLVVALPIYTVASYQNK
                     EVHQGTITDKYNKRQDKEDKFYIVLDDKQVIENSDLFFKGKFDSADIQARLKVGDKVK
                     VKTIGYRIHFLNLYPVLYEVKKVDKK"
     gene            10062..10211
                     /locus_tag="O0293_001635"
     CDS             10062..10211
                     /locus_tag="O0293_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCY0280521.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDYVFR
                     AEVSE"
     gene            10211..10411
                     /locus_tag="O0293_001636"
     CDS             10211..10411
                     /locus_tag="O0293_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HCY0280522.1"
                     /translation="MWITMTIVFAILLLVCISINSDRAREIQALRYMNDYLLDEVVKT
                     KGYNGLKEYRIELKRMNNDIKK"
     gene            10439..10855
                     /locus_tag="O0293_001637"
     CDS             10439..10855
                     /locus_tag="O0293_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCY0280523.1"
                     /translation="MIKIEKHDIKKLEEYIQHIDNYRRELKMREYELLESHEPDNAGA
                     GKSNLPGNPIERCAIKKFSDNRYNTLRNIVNGVDRLIDESDEDTLELLRFRYWDCPIG
                     CYEWEDIAHYFGTSKTSILRRRNALIDKLAKYIGYV"
     gene            11087..11386
                     /locus_tag="O0293_001638"
     CDS             11087..11386
                     /locus_tag="O0293_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCY0280524.1"
                     /translation="MMTKDERIRFYKSKEWQTTRKRVLERDNYECQQCKRDGKLTTYD
                     KSKRKSLDVDHILSLEHHPEFAHDLNNLETLCIKCHNKKEKRFIKKENKWKDEKW"
     gene            11516..>11851
                     /locus_tag="O0293_001639"
     CDS             11516..>11851
                     /locus_tag="O0293_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280525.1"
                     /translation="MEIIVDENLVLKEKERLQVLYKDIPSNKLKVVDGLIIQAARLRV
                     MLDYMWEDIKEKGDYDLFTQSEKAPPYERERPVAKLFNARDAAYQKIIKQLSDLLPEE
                     KEDTETPSDD"
BASE COUNT         4774 a         1707 c         2305 g         3065 t
ORIGIN      
        1 aatgatgtgt agagttttgc tttcagcatc tttcatatgt tcttgtactt taataacttc
       61 accattaatt aaatgtaaat ctaaagaagt aacgatttct ttatctaaat attgagtgag
      121 caatctctta tgcatactta tcacctcctt tcactaggag ataacaacat tatacacgaa
      181 aggaaagata gaaatgccac acattttaaa cgtaacagtt ccaatacctg aaacacatgt
      241 acttatcaca aaagatgaat atgatgagct aattggttat tcattagacc ctgtatggaa
      301 catgagtgac ttaaagaaga aattaaaaat tgcatctgat gagactatca aggacagatt
      361 actatttcat cctagatttg aaaaagaact aagagcgcaa ggaattgtgc attacccaga
      421 tgagagtttt aatcgctgga gatttaacgc aagaaagatg aataaattcg tcgatgagca
      481 tttcaatgaa atatataagg agagaataaa atgagcgaca catataaaag ctacctatta
      541 gcagtgctat gtttcacagt cttagcgatt gtgcttatgc cattgctgta cttcactaca
      601 gcgtggtcaa ttgcaggatt cgcaagcatt ggaacattca tatactacaa agaatacttt
      661 tatgaagaat aaaaaaactg ctacttgcgt caacaagtaa cagtgacaaa cgattaacaa
      721 aattaattca ttttcaatat aaaacgaaac aaggaggaag tcaaccatga ctaaaaatta
      781 taaagatatg actcaagacg aattaagggg tttattggct gaaaagaatg cagaattgtt
      841 tgatttagcg agcgaaatcg atgaagaaac tgaatttgac gttttacttt tttcaaatgt
      901 agggattagc aacggagatt ttacaccgtc gtcacattgt gtgattggga atgttgttga
      961 tattgctaat ttattgaaac gtagagctgt ttaccgagat atcgctgatg ttatcaaaat
     1021 gcgttaaaaa ttctagctat agataatgac aaggaggacg agaacaatgt attacaaaac
     1081 gggtgacgta tgtcgaaaaa taattaatgt agatggcttt gattttcaat taagagttaa
     1141 gaaacgagcg tatagcgtcg aaatggttgt tctagaccat gaaggaaatt caattgacgg
     1201 gttactagtt tctgatgaga acgatctata cacagcttta gatattttga aacaaagtgt
     1261 ttatgaatgg attgaaaata acacagatga acaggacaaa ctaatgaact tagtcatgaa
     1321 atggtaggta taagcatgag agatacagaa agaaatatat tgaatatttt taagacgtta
     1381 ttcgacgaat atactttgtc aaaccaacga gcattattgg aaattgaacg taatcatcac
     1441 ggatacttat cgattaattt cttgcactat cacgacagtt acaaaacaaa caataagctt
     1501 gtgcagatac atgaaatcaa tccagacagc catgaacgaa taaaaaattt aattatcgag
     1561 gtactaagag gtcatcggaa gattaaaaaa ggagcatgag gaaggatatg aaaataaata
     1621 aattaacaat atcgaacttt gccggaatca aagaagaaaa atttaatttt gacggtaaag
     1681 atgcaaaaat atacggcaat aatgcgactg gtaagactac aacagcaacc gcattacaat
     1741 ggttgctttt cgataaaggt ttggacggtt caaccaaatc atttaaccct gtacctttaa
     1801 acgaaaaaaa cgaagaaaat tatgagttaa ttccgactgt tttcgcagaa tttgaaatcg
     1861 acggaaaaat tacgactttt aaaaaagagt cacatcctaa atacacaata aatcaaaaga
     1921 cgaatcgcaa ggaatactca cgaagtcgaa cgaagaaaca atatatcaat gatgaatcaa
     1981 taaaagtaaa ggattataaa gctcgtattg atgaacttat tgatgaagat gtattcaagt
     2041 taattacgaa ccctcaagca tttaacttac tagattggaa gaagcgaaga agtttgttgt
     2101 ttgaaattgc taaaccaatc aatgatgagg atgtcattaa aacaaatgat gattttaaag
     2161 aactaaataa tattcttgga gatcatgaaa ttgaaacaaa gaaaaagatt cttacggaca
     2221 agataaaaca gattaacaaa gatatcaaag atattccgat acgtattaac caaacacaac
     2281 aaaataagca ggatgtacca gaattcgata atgatagata cgcaattatc aaacaagaaa
     2341 ttgagcaact tgaaaatgag cgtatagata ttcaaaacgg taaggaagaa attaatttac
     2401 gtaatcaatt agctgataaa caatcagaat tgaaacgcat agaagacaat aacagcgcaa
     2461 gtaatgagaa caaaatccat gctttaacaa atgaattaca cgttgaaaat ggaacggtag
     2521 caaaccttaa aacgagatta aagcaaaaca aacaacaaat cacgcatgaa gaaaatagac
     2581 gtaatcaatt attagaaaat cataaaggat taaaaagtga tttagaaaaa gctaaaaatc
     2641 aaaaatttga atatcttgat gacaatgtat gtagttgttg tggtcaaaag ttaccagttg
     2701 aacaagtgga tatggcaaga gaaaaagcat tgcagaaatt caatgctagc aaatcgaaag
     2761 aattagaaac aatacaaaca tctatcaatc acattatttc agaaggcaag aaaataaagc
     2821 taatcatcga gaagttagag gatgacaata ataatcttca aattaaaatc aacgaagcag
     2881 aagagcgttc agcaagaata caaaacaaaa ttaataagtt gaaaacaact cacgttgacg
     2941 ttacgcaaac tgacgaatac aaagcagtaa tgttagagat aaatgagatt aatcaaaaac
     3001 gctctaacat caggaaaact attcaagata aagtttcagg aatagatgac aaaataagcg
     3061 aacttactca agaaaaatca gaaattgaag tgtcaagatc aatcgaaaaa tcaaataaac
     3121 atctagatga tgttatttct gaattaagaa atgaagaaga cagattattg gatgaaaaag
     3181 aaaagtattc acatgacctt tatatcttaa aagaatttac aacaacaaaa gtcaaaatgc
     3241 ttactgaaaa catcaataat gaatttgaaa ttgctgaatt taagctattc aataccttag
     3301 ttaacggcga attagaagaa acatgttcca caacggttaa cggcgtcgaa tacgacagcg
     3361 gtttaaataa cgcctcaaga attaatgttg gcttagatat catcaataca ctgtcaaaac
     3421 attttaaagt tacagcgcca atatttattg ataatgctga atcagtaaca gagcttatca
     3481 aaacagaatc acaacaaatt caattgatag taaatgaaca agataaaaaa ttaagaatgg
     3541 agactatata aaatgactga aaataataaa ttacaaacta ttgaacaaca attagtacaa
     3601 gaaaagaacg tatctgacaa cgtattaaac aaagtgagag ttttagagtc acaaggcaat
     3661 ttggaattgc caaatgatta ttcaccaagt aatgccatga aacaagcatg gttacaaatc
     3721 agccaagata acaaattaat gagttgtaac gatacaagca aagcaaatgc cttattagac
     3781 atggtaacgc aaggtttaaa tccagctaaa aatcaatgct actttattcc ttacggcaac
     3841 aaaatgcagt tacaacgtag ctatcacggt aatgtaatga tgttaaaacg tgatgcaggt
     3901 gctcaagatg ttgttgctca agtgatttat aaaggcgata cattcaagca agaaatggga
     3961 gaaacaggac gtatcaaagc gattaaacac gaacaagact tctttaacat cgacaaagaa
     4021 aacattatcg gtgcgtactg cacaatcgta tttaatgatg gacgagataa ctatattgaa
     4081 gtcatgacta ttgaacaaat taaacaagca tggatgcagt catcaatgat taaagatgaa
     4141 aaagcattac aaaattctaa aacacataat aatttcaaag aagaaatggc taaaaaaaca
     4201 gttatcaata gagctgctaa acgttatatc aacacatcaa cagatagcaa tcttttcaaa
     4261 tacgcacaag aatccgaaca acgtcaacgc aaagaagtgt tggacgcaga agttgaagaa
     4321 aatgcaaatc aagaacaatt ggactttgaa caaccagttc ttgaagaagc acaatacaca
     4381 gaattagaaa atgataagcc tattgatgta tctgactttg aagaaataaa agaacctgca
     4441 acagaaaaag aaagcgaaga agagccattt taattgaaac aatagcaact ggttcaagtg
     4501 gtaactgcta cgtcttaaat gatggacgta ctacgttact acttgaggca ggtataaaat
     4561 ttgaacgtgt tcaaaagcat ttcaaatata aaacaagaca tatagcaggg tgtcttatca
     4621 cacacgaaca tggtgatcat gcaaagtata caaagcagtt tgtcgacaat ggtgtaatca
     4681 gctatatgac tgctggaaca caacaagcta tgaattttga aagtcatcgc ttatgcacga
     4741 ttaaggcaaa gcaagagctg cgaataggta catggtcaat tctaccgttt gacatcgaac
     4801 atgatgctaa cgagcctgtg gctttcttat tacaaagcac actaggttat aaggttctgt
     4861 atgttactga tacaaagtat ttgaaataca aatttaatgg cattacgcac atgatgctag
     4921 aagttaatta tatctatgaa caaatgcaag aaaacataaa aaacggcagt gtgcacagca
     4981 cattagcaaa cagaattatg gagtctcatt ttagcttaga acatgctatc ggaatgttaa
     5041 aagcaaatga tttaactaga ctcgaagaaa tacatttaat tcatttaagt agtcaaaatt
     5101 caaatgcaaa atacattaaa agtgaaatac aaaaagtgac gggcgcgccc gtttattttg
     5161 gaggtttata aatgctaaac agaacaatat tagttggtcg tttaactaga gacccagaat
     5221 taagaaccac tcaaaatggt gtaaatgtag catcattcac attagcagtt aaccgtacat
     5281 ttacaaatgc acaaggcgag cgcgaggcag actttattaa tatcatcgta tttaaaaaac
     5341 aagcagagaa cgttaataaa tacctatcta aaggatcgtt ggcgggcgta gatggtaggt
     5401 tacaaacgcg gaactatgaa aataaggaag gtcaacgtgt atacgttacg gaagttattg
     5461 ctgatagtat tcaattttta gaaccgaaaa actcaaatga cactcaacaa gatttatatc
     5521 aacaacaagt acaacaaaca cgtggacaat cgcaatattc aaataacaaa ccagtaaaag
     5581 ataatccgtt tgcgaatgca aatggtccga ttgaactaaa tgatgatgat ttaccattct
     5641 aatttaaccg gtttgaaagt gaggtgtgta tatgactggt tggataagta ttgatcgctc
     5701 aattcaaaat cattggctat ttaaagaaaa gagaacattt tcaaagtttg aagcatggat
     5761 atatttactc atggaagcga atcattcaaa ggcaaaagtg cctataggaa accaaattgt
     5821 aaccgttgaa agaggacaaa gattaacatc tattttgacc ttgtctgacc tttttaactg
     5881 gtcacgattt aaagtgaaaa ccttccttga cttactcgag agtgatggaa tgttagaagt
     5941 caaaacaaca tcaaaatata cccttataac cattgtcaat tatgactttt atcaaagtga
     6001 gcagggcagg aaccaacatc aaaacgacat caaaccaaca tcaaaacaac atcagtcaaa
     6061 catcaaccca acatcaaaac aacatcaaac caacacaaac aataatgata ataaagataa
     6121 taatgaaaag aatgtgaata atgagaagaa gaagacaacc gccttcgact tcttccaaga
     6181 taacggattc ggtttcataa cttcttacaa tttagacgat ttaaattatt atcttgattc
     6241 atttgaaaat gattcagatg aaatagttac cgcatcactt aaaatcgcta aagacagaaa
     6301 caaagttact tggggatatg ctaaaagcat tttgaataca tggcttaatg caaacttgaa
     6361 atctattgaa caagtacgtg catttgaaaa gcaacaactt gaaagcaaaa aacaaaatta
     6421 taaacctttc gttaaacaat caaaagaaaa aacacccaaa tggctcacag acagcacgag
     6481 agaaacgaaa acgccggaag tagatgaaaa ccttgagaaa gacagagaag cttttattaa
     6541 gcgtctaaat agcaaatggg agtgattgaa aatggatgca tttgataaat actatctatt
     6601 tgatcatgac ggcaacaaaa tgttttcagt tacaccacat tttaaagatg gacggcattt
     6661 agttgttgga ttaaaacaca caaaattcaa tggtcgacgt tggtacttag atgattatga
     6721 attaaaaaca cttattgata atgaacaaat ggagttagga caccaaacaa gcttatttga
     6781 atatatatga gggattacat ggagatagaa attaaattta atgaagtgtt taatgcgccg
     6841 atgggctcgc ctcgaccgcg ttttagcaca aaaggtagat atgcacacac atatatgcct
     6901 acaaaatata cagaacataa aaaatattta caaaatcaaa tgccaaagct aaatctagaa
     6961 aatgcattaa aaattgaatt agagttttac tttactccac ctaaatcatg gtcgaagaaa
     7021 aagaaaactc aagcgattgg acaattaaaa gtaactaaac cagatatcga taatttgatg
     7081 aagacagttc tagacgcttg taacaattat ttgtggaaag acgataacca aattacagaa
     7141 ataactagct caaagcgtta tggaattgag cccaaaataa tcatacgaat agaagaaata
     7201 taagaggtgg ataaaatggc gagaaaagca aggattgtaa caataaatga taaaccatat
     7261 aggtttacca aatctgaaat ggaattaata gaaagtcacg gtataaccgc tggaatggtt
     7321 tctaagagag taaaagacgg ttgggaacta catgaagcaa tggacgcacc agaaggtacg
     7381 cgtttaagcg agtacagaga aaagaaaaca atagaaagac tggaacaagc tagactcgaa
     7441 cgcaaattgg aaagaaagcg aaagaaagag gctgagctaa gaagaaagaa gccacatttg
     7501 tttaatgtac cacagaaaca tccaagagga cgttatgcgt gctacctgat ggaaaacgac
     7561 atattcgtga aagttaagaa gtagatcatg acagataacg cacgcaaaga atacctaaat
     7621 caattctttg gatctaagag atatctgtat caggataacg aacgagtggc acatattcat
     7681 gtagtaaacg gcacttatta ctttcatggg catatcgtac caggttggca aggcgtgaaa
     7741 aagacatttg atacagctga agagcttgaa acatatataa agcaacaggg tttggaatat
     7801 gaggaacaga agcaactaac tttattttag aggaggttat gaaagtgaac tatgaaacag
     7861 gattccaaat aggcgtaatg gaagctaggt tgaagaagat gagaaaacaa cgtgatgagt
     7921 acaagaagca acgtgacgag cttattgggg atatagctaa gttaagagag cgtaacgaag
     7981 agctggagaa catgtggcgc acgcttaaaa atgaattgtt tggaagatac gaattttacc
     8041 gttttagact tagcgaacta cagattgaga gcagagcgaa caaggaagta gctatatata
     8101 gaagagctga aatcaactta agtgttatat tgtgccgaat ggacaaacta gacggaacaa
     8161 atgagttcta cgaattttta gggcaaatgg aggatgacac aaatgaataa ccgcgaacaa
     8221 atcgaacaat cagttatcag tgctagcgcg tataacggca atgacacaga gggattgcta
     8281 aaagagattg aagacgtgta taagaaagcg caagcgtttg atgaaattcg cgaagctatt
     8341 aatgcgcaat gggttgagta tccagaagac tgggcgtcag aggttttgag agaagtaaga
     8401 gagcttgaat atgaggagga acaggaaaat gaaaattaat gaatctttga agaaattaaa
     8461 agaaaaagga tacaaagaaa atgaagataa agctattttt aatttagctg atggtacgct
     8521 agaaatttac atcgaccatg acgaaaaaac aataattaca gaattccatg atttaaaggt
     8581 attcgtgtca gaagatttaa aagataaaag tatggagagc gttatgtatg aattggctgg
     8641 aattgatgag gaggacaagg aaaatgacta acatcctaca agtaaagcta ttatcagaaa
     8701 acgctagaat gccagaacga aatcataaga cggatgcagg ttatgacata ttctcagctg
     8761 aaactgtcgt acttgagcca caagaaaagg cagtgatcaa aacagatgta gctgtaagca
     8821 taccagaggg ctatgtcgga ctattaacta gccgtagtgg tgtaagtagt aaaacgcatt
     8881 tagtgattga aacaggcaag atagacgcgg gatatcacgg caatttaggg attaatatca
     8941 agaatgataa tgaaacgtta gagagtgagg atatgagtaa ctttggtcgg agtcctgctg
     9001 ggatagatgg aaagtatgcc cgactacctg taacagataa aattttatgt atgaatggta
     9061 gttatgtcat aaacaaaggc gacaaactag ctcaattggt tattgtgcct atatggacac
     9121 ctgaactaaa gcaagtggag gaattcgaga gtgtttcaga acgtggagca aaaggcttcg
     9181 gaagtagcgg agtgtaaaga catattagat cgagtcaagg aggttttggg gaagtgagcg
     9241 acatgttaga aatattttta atagggtttg gcgtttatct cttttatcgc atagcaatta
     9301 tttttcttaa gagtaaaaag actatacaca caaacatata tgaaatgtta atgcttgcta
     9361 ctatctttat gatatctaca tttgcttata aacatcaaaa gacgcatatc ttaatagcat
     9421 ttttagtaat gttttttatg agtaagctca aacaagttca agggagctat gaggaatgac
     9481 acaatactta gtcacaacat tcaaagattc aacaggacgt aaacatacac acataactaa
     9541 agctaagagt aatcaaaggt ttacagttgt tgaggcagag agtaaagaag aagcgaaaga
     9601 gaagtacgag aaacaagtta aaagggatgc agttattaaa gtgggtcagt tgtttgaaaa
     9661 tataagggag tgtgggaaat gattaaaaaa cttaaaaata tggatgggtt cgacatcttt
     9721 attgttggaa tactgtcatt attcggtata accgcattgc tacttgttgt cgcattgcct
     9781 atctatacag tggctagtta ccaaaacaaa gaagtacatc aagggacaat tacagataaa
     9841 tataacaaaa gacaagataa agaggacaaa ttctatattg tattagatga taaacaagtc
     9901 atcgaaaact cagacttatt cttcaaagga aagtttgata gcgcagacat acaagctagg
     9961 ttaaaagtag gtgataaagt aaaagttaag acgattggat atagaataca ctttttaaat
    10021 ttatatccgg tcttatacga agtaaagaag gtagataaaa aatgattaag caaatactaa
    10081 gattattatt cttactagcg atgtatgagt taggtaagta tgtaactgag caagtatata
    10141 ttatgatgac ggctaatgat gatgtagagg cgccgagtga ttacgtcttt cgagcggagg
    10201 taagtgagtg atgtggatta ctatgactat tgtatttgct atattgctat tagtttgtat
    10261 cagtattaat agtgatcgtg caagggagat acaagcgctc agatatatga atgattatct
    10321 acttgatgaa gtagttaaaa ctaaaggata caacgggtta aaagaataca ggattgaatt
    10381 aaagcgaatg aataacgata ttaaaaagta atttatatta tcggaggtat tacattgaat
    10441 gataaagatt gagaaacacg atatcaaaaa gcttgaagaa tacattcagc acatcgataa
    10501 ctatcgaaga gagttgaaga tgcgagaata tgaattactt gaaagtcatg aaccagataa
    10561 tgcgggagct ggcaaaagta atttgccggg taacccgatt gaacgatgtg caataaagaa
    10621 gtttagtgat aacaggtaca atacattaag aaatatagtt aacggtgtag atagattgat
    10681 agatgaaagt gatgaggata cgcttgagtt attaaggttt agatattggg attgtcctat
    10741 tggttgttat gaatgggaag atatagcaca ttactttggt acaagtaaga caagtatatt
    10801 acgtagaagg aatgcactga tcgataagtt agcaaagtat attggttatg tgtagcggac
    10861 ttttacccta tgtaagtccg cattaaaaca gtttattatg ttagtatcag attaatattt
    10921 aaagttatta aatgctaata cgacgcatga acaagaggcg catcactatg tgatgtgtct
    10981 ttttatttat gaggtatgaa catgttcaaa ctaatagtaa atacattact acacatcaag
    11041 tatagatgcg tcttgatact acttaagtta tataaggtga aacattatga tgactaaaga
    11101 cgaacgcata cgattctata agtctaaaga atggcaaaca acaagaaaaa gagtgctaga
    11161 aagagataat tatgaatgtc aacaatgtaa gagagacggc aagttaacga catatgacaa
    11221 aagcaagcgt aagtcgttgg atgtagatca tatattatcg ctagaacatc atccggagtt
    11281 tgctcatgac ttaaacaatt tagaaacact gtgtattaaa tgtcacaaca aaaaagaaaa
    11341 gagatttata aaaaaagaaa ataaatggaa agacgaaaaa tggtaaatac ccccgggtca
    11401 aaaaaatcaa aagcgatcaa aatacttggg gaacggggag gggctcgact tcgcgataat
    11461 tttaaaaatc catgtatacc ccccctctta taaccatttt aaggcaggtg atgaaatgga
    11521 gattatagtt gatgaaaact tagtgcttaa agaaaaagaa aggctgcaag tattatataa
    11581 agacatacct agcaataaat taaaagtagt tgatggttta attattcaag cagcaaggct
    11641 acgtgtaatg cttgattaca tgtgggaaga cataaaagaa aaaggtgact atgatttatt
    11701 tactcaatct gaaaaggcgc caccatatga aagggaaaga ccagtagcca aactatttaa
    11761 tgctagagat gctgcatatc aaaaaataat caaacaatta tcggatttat tgcccgaaga
    11821 gaaagaagac acagaaacgc catctgatga t
//
LOCUS       DAKTIO010000084        11753 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.084, whole genome shotgun sequence.
ACCESSION   DAKTIO010000084 DAKTIO010000000
VERSION     DAKTIO010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11753
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.084"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            178..1989
                     /locus_tag="O0293_001640"
     CDS             178..1989
                     /locus_tag="O0293_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCY0280526.1"
                     /translation="MDTKDFKRLEKMYSPRYLPGLDGLRAFAVIGIIIYHLNAQWLSG
                     GFLGVDTFFVISGYLITSLLISEYYRTQKIDLLEFWKRRLKRLIPAVLFLICVVLTFT
                     LIFKPELIIQMKRDAIAAIFYVSNWWYISQNVDYFNQFAIEPLKHLWSLAIEEQFYLL
                     FPLVITFLLHRFKPRNIIQTLFIVSLISLGLMIVIHFITGDNSRVYFGTDTRLQTLLL
                     GCILAFIWPPFALKKDISKKIVVSLDIIGISGFAVLMTLFFIVGDQDQWIYNGGFYII
                     SFATLFIIAIAVHPSSLFAKFLSMKPLLIIGKRSYSLYLWHYPIIVFVNSYYVQGQIP
                     VYVYIIEILLTALMAEISYRFIETPIRKKGFKAFAFLPKKKGQFARTVLVILLLVPSI
                     VVLSGQFDALGKQHEAEKKEKKTEFKTTKKKVVKKDKQEDKQTANSKEDIKKSSPLLI
                     GDSVMVDIGNVFTKKIPNAQIDGKVGRQLVDATPIVKSQYKDYAKKGQKVVVELGTNG
                     AFTKDQLNELLDSFGKADIYLVSIRVPRDYEGRINKLIYEAAEKRSNVHLVDWYKASA
                     GHPEYFAYDGIHLEYAGSKALTDLIVKTMETHATNKK"
     gene            2350..3117
                     /locus_tag="O0293_001641"
     CDS             2350..3117
                     /locus_tag="O0293_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0280527.1"
                     /translation="MKKIVTATIATAGLATIAFAGHDAQAAEQNNNGYNSNDAQSYSY
                     TYTIDAQGNYHYTWTGNWNPSQLTQNNTYYYNNYNTYSYNNASYNNYYNHSYQYNNYT
                     NNSQTATNNYYTGGSGASYSTTSNNVHVTTTAAPSSNGRSISNGYASGSNLYTSGQCT
                     YYVFDRVGGKIGSTWGNASNWANAAASSGYTVNNTPKVGAIMQTTQGYYGHVAYVEGV
                     NSNGSVRVSEMNYGHGAGVVTSRTISANQAGSYNFIH"
     gene            3292..3780
                     /gene="crtO"
                     /locus_tag="O0293_001642"
     CDS             3292..3780
                     /gene="crtO"
                     /locus_tag="O0293_001642"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl-4,4'-diaponeurosporenoate
                     acyltransferase"
                     /protein_id="HCY0280528.1"
                     /translation="MKKYIKTAFFCSMYWLIVQLNIANLGTRIPDKYFRQKYIIFKSF
                     NFEKHGKFWNKWFYVRKWKHKILDGHQLNQNIYDQRHLMTINTDEIEKMIIETKRAEL
                     IHWISILPVIIFNKGPRLVKYINIFYAMIANVPIIIVQRYNRPRLTQLLRILKRRGER
                     HD"
     gene            3773..5266
                     /locus_tag="O0293_001643"
     CDS             3773..5266
                     /locus_tag="O0293_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0280529.1"
                     /translation="MTKHIIVIGGGLGGISAAIRMAQSGYSVSLYEQNNHIGGKVNRH
                     ESDGFGFDLGPSILTMPYIFEKLFEYSKKQMSDYVTIKRLPHQWRSFFPDGTTIDLYE
                     GIKETGQHNAILSKQDIEELQNYLNYTRRIDRITEKGYFNYGLDTLSQIIKFHGPLNA
                     LINYDYVHTMQQAIDKRISNPYLRQMLGYFIKYVGSSSYDAPAVLSMLFHMQQEQGLW
                     YVEGGIHHLANALEKLAREEGVTIHTGARVDNIKTYQRRVTGVRLDTGEFVKADYIIS
                     NMEVIPTYKYLIHLDTQRLNKLEREFEPASSGYVMHLGVACQYPQLAHHNFFFTENAY
                     LNYQQVFHEKVLPDDPTIYLVNTNKTDHTQAPVGYENIKVLPHIPYIQDQPFTTEDYA
                     KFRDKILDKLEKMGLTDLRKHIIYEDVWTPEDIEKNYRSNRGAIYGVVADKKKNKGFK
                     FPKESQYFENLYFVGGSVNPGGGMPMVTLSGQQVADKINAREAKNRK"
     gene            5272..6399
                     /gene="crtQ"
                     /locus_tag="O0293_001644"
     CDS             5272..6399
                     /gene="crtQ"
                     /locus_tag="O0293_001644"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,4'-diaponeurosporenoate glycosyltransferase"
                     /protein_id="HCY0280530.1"
                     /translation="MKWLSRILTVIVTMSMACGALIFNRRHQLKAKTLNFNHKALTII
                     IPARNEEKRIGHLLHSIIQQQVPVDVIVMNDGSTDETARVARSYGATVVDVVDDTDGK
                     WYGKSHACYQGVTHACTNRIAFVDADVTFLRKDAVETLINQYQLQGEKGLLSVQPYHI
                     TKRFYEGFSAIFNLMTVVGMNVFSTLDDGRTNQHAFGPVTLTNKEDYYATGGHKSANR
                     HIIEGFALGSAYTSQSLPVTVYEGFPFVAFRMYQEGFQSLQEGWTKHLSTGAGGTKPK
                     IMTAIVLWLFGSIASILGLCLSLKYRQMSVRKMVALYLSYTTQFIYLHRRVGQFSNLL
                     MVCHPLLFMFFTKIFIQSWKQTHRYGVVEWKGRQYSISKEQ"
     gene            6436..7299
                     /locus_tag="O0293_001645"
     CDS             6436..7299
                     /locus_tag="O0293_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HCY0280531.1"
                     /translation="MTMMDMNFKYCHKIMKKHSKSFSYAFDLLPEDQRKAVWAIYAVC
                     RKIDDSIDVYGDIQFLNQIKEDIQSIEKYPYEHHHFQSDRRIMMALQHVAQHKNIAFQ
                     SFYNLIDTVYKDQHFTMFETDAELFGYCYGVAGTVGEVLTPILSDHETHQTYDVARRL
                     GESLQLINILRDVGEDFDNERIYFSKQRLKQYEVDIAEVYQNGVNNHYIDLWEYYAAI
                     AEKDFQDVMDQIKVFSIEAQPIIELAARIYIEILDEVRQANYTLHERVFVDKRKKAKL
                     FHEINSKYHRI"
     gene            7311..8819
                     /locus_tag="O0293_001646"
     CDS             7311..8819
                     /locus_tag="O0293_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0280532.1"
                     /translation="MKIAVIGAGVTGLAAAARIASQGHEVTIFEKNNNVGGRMNQLKK
                     DGFTFDMGPTIVMMPDVYKDVFTACGKNYEDYIELRQLRYIYDVYFDHDDRITVPTDL
                     AELQQMLESIEPGSTHGFMSFLTDVYKKYEIARRYFLERTYRKPSDFYNMTSLVQGAK
                     LKTLNHADQLIEHYIDNEKIQKLLAFQTLYIGIDPKRGPSLYSIIPMIEMMFGVHFIK
                     GGMYGMAQGLAQLNKDLGVNIELNAEIEQIIIDPKFKRADAIKVNGDIRKFDKILCTA
                     DFPSVAESLMPDFAPIKKYPPHKIADLDYSCSAFLMYIGIDIDVTDQVRLHNVIFSDD
                     FRGNIEEIFEGRLSYDPSIYVYVPAVADKSLAPEGKTGIYVLMPTPELKTGSGIDWSD
                     EALTQQIKEIIYRKLATIEVFEDIKSHIVSETIFTPNDFEQTYHAKFGSAFGLMPTLA
                     QSNYYRPQNVSRDYKDLYFAGASTHPGAGVPIVLTSAKITVDEMIKDIERGV"
     gene            9246..10400
                     /locus_tag="O0293_001647"
     CDS             9246..10400
                     /locus_tag="O0293_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCY0280533.1"
                     /translation="MISNKLANIPDSYFGKTMGRKIEHGPLPLINMAVGIPDGPTPQG
                     IIDHFQKALTIPENQKYGAFHGKEAFKQAIVDFYQRQYNVTLDKEDEVCILYGTKNGL
                     VAVPTCVINPGDYVLLPDPGYTDYLAGVLLADGKPVPLNLEPPHYLPDWSKVDSQIID
                     KTKLIYLTYPNNPTGSTATKEVFDEAIAKFKGTDTKIVHDFAYGAFGFDAKNPSILAS
                     ENGKDVAIEIYSLSKGYNMSGFRVGFAVGNKDMIQALKKYQTHTNAGMFGALQDAAIY
                     ALNHYDDFLEEQSNVFKTRRDRFEAMLAKADLPFVHAKGGIYVWLETPPGYDSEQFEQ
                     FLVQEKSILVAPGKPFGENGNRYVRISLALDDQKLDEAAIRLTELAYLYE"
     gene            10423..11421
                     /locus_tag="O0293_001648"
     CDS             10423..11421
                     /locus_tag="O0293_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCY0280534.1"
                     /translation="MTKIKIMSVRDEDMPYIKAWAEKHHVEVDITKEALTDDNVEGVA
                     GYDGLSLSQQIPLSEHVYKRLNELGIKQIAQRSAGFDTYDLELANKYNFIVSNVPSYS
                     PNSIAEFAVNQAINVVRHFNQIQTKVREHDFRWEPTILSKSIKDLKVAVIGTGRIGRV
                     VADIFANGYQSDVVAYDPFPNAKIATYVDYKDTIEEAVEGADIVTLHVPATKYNHYLF
                     NAELFKHFKKGAVFVNCARGSLVDTKALLDALDNGVIKGAALDTYEFERKLFPSDQRG
                     KTLNDPLLESLIDREDVILTPHIAFYTEAAVKNLIVDALDATLDVLQTGDTRLRVN"
     gene            complement(11511..11717)
                     /gene="copZ"
                     /locus_tag="O0293_001649"
     CDS             complement(11511..11717)
                     /gene="copZ"
                     /locus_tag="O0293_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HCY0280535.1"
                     /translation="MSQEILNVEGMSCGHCKSAVESALNNIDGVTSADVNLENGQVSV
                     QYDDSKVAVSQMKDAIEDQGYDVV"
BASE COUNT         4043 a         1727 c         2292 g         3691 t
ORIGIN      
        1 tatatgtggt ttgcagtaat gaaatcaact gtaataattg ttttctactt aataaaaaat
       61 ttaaaactat cttaattaag ggttaaatca attaatgaaa atcagtgcaa tattttattt
      121 tcaaaaatat tgtgatatgc tacaaaatag agaaaaataa atggggcgtt tattaacatg
      181 gatacaaaag actttaaacg tttagaaaaa atgtattccc cgcgatactt acctggatta
      241 gatggattga gggcattcgc agttatagga atcattattt atcacttgaa tgcacaatgg
      301 ttaagtgggg gctttttagg agtagataca ttcttcgtta tttcaggtta tttaataaca
      361 agtttgttga taagtgagta ttatcggacg caaaaaatcg atttgctaga gttttggaag
      421 cgacgattga aacgactcat tccggcagtg ttatttttaa tctgtgtcgt gcttacgttc
      481 acactgatat ttaaaccgga attgattata caaatgaaac gagatgctat tgcagctata
      541 ttctatgttt caaactggtg gtacatttca cagaatgtag attattttaa ccaatttgct
      601 attgaaccac tcaaacattt atggtcttta gccattgaag aacaatttta cttgcttttc
      661 ccattggtta tcacgttctt attacataga tttaaaccga gaaatattat tcaaacgcta
      721 tttattgtat cgttgatttc tttaggactt atgatagtga ttcatttcat cactggagat
      781 aattcacgtg tgtattttgg gacagataca cgactgcaaa ctttattgct tggttgtata
      841 ttagcattta tttggcctcc gtttgctttg aaaaaagata tttctaaaaa gattgtcgta
      901 tcattagata ttatagggat atctggtttt gcggttctaa tgactttgtt ctttatagtt
      961 ggagaccaag atcaatggat ctataatgga ggattttaca ttatatcatt tgcaacttta
     1021 ttcattattg caattgcggt acatccttct agtttatttg ctaaattttt aagtatgaaa
     1081 cctttactaa ttataggtaa acgatcatat agcttatact tatggcatta tcctatcatt
     1141 gtttttgtga acagttatta cgtacaagga caaataccgg tatacgttta tattatagaa
     1201 attttgttaa cagcgttaat ggctgaaatt tcgtatcgct ttattgaaac acctatacgt
     1261 aaaaaaggat ttaaagcttt tgcattttta cctaaaaaga aggggcaatt tgctagaaca
     1321 gtgttagtta tcctattatt ggttccgtct atcgttgtgc tcagtggaca gtttgatgca
     1381 cttggcaaac aacatgaagc cgagaagaaa gagaagaaga cggaatttaa aacaacgaag
     1441 aaaaaagtcg ttaaaaaaga taagcaagag gataagcaga cagcgaatag caaagaggat
     1501 attaaaaagt catcaccact attaattggt gactcggtca tggtggatat tggtaatgtc
     1561 tttactaaga aaataccaaa tgcacaaatt gatggtaaag ttggacggca actcgttgat
     1621 gctacaccaa ttgtgaaatc gcaatataaa gactatgcta aaaaaggtca aaaagttgta
     1681 gtagagcttg gtacaaatgg ggcatttacg aaagatcaat taaatgaact attggatagt
     1741 tttggaaaag cagacatata tttagtttct attagagtac ctagagatta tgaaggtaga
     1801 ataaataaat taatttatga ggcagctgaa aagcgctcta atgtacatct agtcgattgg
     1861 tataaagctt ctgcaggtca tccggaatac tttgcatatg acggtattca cttagaatat
     1921 gcaggtagta aagcgctgac tgatttgatt gtaaaaacga tggaaacaca tgctacaaat
     1981 aagaaataat ttgatgcact aaacttttga aatattacat tacttctgat atttattatc
     2041 aaaaatgatg tatttcatta aaagtttagt gcttttttat tttcaaatcc catagtaacg
     2101 gtgcagaaaa agtgttgtaa acattctaat tggtatatta cattcaatga agctttatta
     2161 ggaacagatt acattatgat aacaaagccc gcaagacacc taatctctgt tatagtttgt
     2221 tttgtcgcaa aactataaaa gttataattg tttgcatact aaaaaaataa aaaatataaa
     2281 atttaaaata attgagtcgc taatgactat atgtatagag tgttttgatt attgggagga
     2341 tatttaatta tgaaaaaaat cgttacagct acaatcgcta cagcaggact tgccactatc
     2401 gcatttgcag gacatgatgc acaagccgca gaacaaaata acaatggata taattctaat
     2461 gacgctcaat catacagcta tacgtataca attgatgcac aaggtaatta tcattacact
     2521 tggacaggaa attggaatcc aagtcaatta acgcaaaaca acacatacta ctacaacaac
     2581 tacaatactt atagttataa caatgcatct tacaataact actataatca ttcatatcaa
     2641 tacaataact atacaaacaa tagtcaaaca gcaacaaata actattatac tggtggttca
     2701 ggtgcaagtt atagcacaac aagtaataat gttcatgtga ctacaactgc agcgccatct
     2761 tcaaatggtc gttcaatttc taatggttat gcatcaggaa gtaacttata tacttcagga
     2821 caatgtactt attatgtatt tgatcgtgtt ggtgggaaaa ttggttcaac atggggtaac
     2881 gcaagtaatt gggctaacgc agctgcatca tctggctata cagtgaacaa tacaccaaaa
     2941 gttggtgcta tcatgcaaac aacacaaggc tattacggtc atgttgctta cgttgaaggc
     3001 gttaacagca acggttctgt tcgtgtttca gaaatgaact atggacatgg tgctggtgtg
     3061 gttacgtctc gtacaatttc agcaaaccaa gcaggttcat ataatttcat tcattaatca
     3121 aatgtaaatc aaatgacgtc aatattctct aacatgagag tattggcgtt tttgttttat
     3181 ataaatataa atgagagcgg tttattcact gatctttagg gaactaagta ataaagtgat
     3241 aatttatact atgtcagtat gattgagagt gattcaattt agatgaaaac catgaaaaaa
     3301 tatattaaaa cagcattttt ttgtagtatg tattggttaa ttgttcaact aaatatagca
     3361 aatttaggta caagaattcc tgataagtat tttcgtcaga agtacataat atttaaatca
     3421 tttaactttg agaagcatgg aaaattttgg aacaaatggt tttacgtaag aaaatggaaa
     3481 cataagattt tagatggtca tcagcttaat caaaatatat atgatcagcg tcatttaatg
     3541 acaatcaata ctgatgaaat tgaaaaaatg attatagaga caaagagggc agagttgatt
     3601 cattggatat cgatacttcc agtcatcata ttcaataaag gccctcgttt agtaaagtat
     3661 ataaatattt tctatgcaat gatagctaat gttccaatca ttattgtgca acgctataat
     3721 cgaccgagat taacgcagtt actacgcata ttaaaacgaa gaggtgaacg tcatgactaa
     3781 acatatcatc gttattggtg gtggcttagg tgggatttct gcagcaattc gaatggcaca
     3841 aagtggctat tcggtctcat tatatgaaca aaataatcat ataggaggca aagtgaatcg
     3901 tcatgaatca gatggctttg gctttgattt aggtccatct attttaacga tgccttatat
     3961 ttttgaaaaa ttattcgaat atagcaagaa gcaaatgtca gactacgtta caatcaagcg
     4021 attgccacat caatggcgta gcttttttcc agatggaacg actatcgatt tgtatgaagg
     4081 tattaaagaa acaggtcagc ataatgcgat attgtcgaaa caggatatag aggaactgca
     4141 aaattatttg aattatacaa gacgaatcga tcgtattact gaaaaagggt atttcaacta
     4201 tggtttagat acactatctc aaattattaa atttcatggg ccattaaatg ctcttattaa
     4261 ttatgattat gtacatacta tgcaacaggc catagacaag cgtatctcga atccatactt
     4321 gcgacaaatg ttaggctatt ttatcaaata tgtaggttct tcatcatacg atgcgccagc
     4381 tgtattatct atgttattcc atatgcaaca agagcaaggc ctttggtatg tagaaggtgg
     4441 aatccatcat ttagccaatg ccttggaaaa gctagcgcgt gaagaaggtg tcacaattca
     4501 tacaggtgca cgtgtggaca atattaaaac atatcaaaga cgtgtgacgg gtgtcagatt
     4561 agatacaggt gagtttgtaa aggcagatta tattatttca aatatggaag tcatacctac
     4621 ttataaatat ttaattcacc ttgatactca acgattaaac aaattagaga gggaatttga
     4681 gccggcaagc tcaggatatg tgatgcattt aggtgttgct tgccaatacc cgcaattagc
     4741 acatcataat ttctttttta cggaaaatgc ttatctcaat tatcaacaag tttttcatga
     4801 aaaggtattg ccagatgatc cgaccattta tctagtaaat acgaataaaa ctgatcacac
     4861 acaagcgcca gtaggttatg aaaatatcaa agtcttacca catattccat atattcaaga
     4921 tcagcctttt accactgaag attatgcgaa gtttagggat aaaattttgg ataaattaga
     4981 aaaaatggga cttactgatt taagaaaaca cattatttat gaagatgttt ggacaccgga
     5041 ggatattgaa aaaaattatc gttctaatcg tggtgcaata tatggtgttg tagcagataa
     5101 aaagaaaaac aaaggattta aatttcctaa agaaagtcag tattttgaaa acttgtactt
     5161 tgtaggtgga tcagtaaatc ctggtggtgg catgccaatg gttacattaa gtgggcaaca
     5221 agtcgcagac aaaataaacg cgcgagaagc gaagaatagg aagtgatatc tatgaaatgg
     5281 ttatcacgaa tattaacagt aatagtgacc atgtctatgg cgtgtggtgc attgatattt
     5341 aatcgtagac atcagctaaa ggcgaaaacg ctgaacttca atcataaagc attaacaatt
     5401 attattccgg ctagaaacga agaaaaaaga ataggtcatt tactacattc gataatacaa
     5461 cagcaagttc cagtagatgt cattgttatg aatgacggat cgacagatga aacagcacgt
     5521 gtagcacgtt catatggtgc gactgtagta gatgttgttg atgatactga cggcaaatgg
     5581 tatgggaaat cacatgcttg ttatcaaggt gtgacgcatg catgtacgaa tcgcattgcc
     5641 tttgtagatg ctgatgtaac tttcttaagg aaagatgctg ttgaaacgtt gattaatcag
     5701 tatcaattac aaggtgaaaa aggattgtta agcgtacagc cttatcatat aacaaagcgt
     5761 ttctacgaag ggttttcagc gatatttaat ttaatgacag tcgttggtat gaatgtattt
     5821 tctaccttag acgacggtcg gactaaccag catgcatttg gaccggtgac attaacaaat
     5881 aaagaagatt attatgcaac tggaggtcat aaaagtgcaa accgtcatat tattgaagga
     5941 tttgctttag gaagtgcata tacttcacaa tcattgcccg taacagttta tgaagggttt
     6001 ccatttgttg catttcgcat gtatcaagaa ggatttcagt cattacaaga aggatggaca
     6061 aagcatttgt caactggggc aggtggcaca aagcctaaga tcatgacagc aattgtgttg
     6121 tggttgtttg gttctatagc gagtatttta gggctatgtc ttagtttaaa atatcgccaa
     6181 atgtctgtaa gaaaaatggt agcactttac ttgagctata ctacacaatt tatttatctg
     6241 catcgaaggg tcggccaatt ttctaattta ttaatggtat gtcatccatt gttatttatg
     6301 ttttttacta aaattttcat ccaatcttgg aaacaaacgc atcgttatgg tgtagttgaa
     6361 tggaaaggtc gtcaatattc tatatctaaa gaacaataaa tcaaggtaat ggcatttcaa
     6421 tataggagga ctagtatgac aatgatggat atgaatttta aatattgtca taaaatcatg
     6481 aagaaacatt caaaaagctt ttcttacgct tttgacttgt taccagaaga tcaaagaaaa
     6541 gcggtttggg caatttatgc tgtgtgtcgt aaaattgatg acagtataga tgtttatggc
     6601 gatattcaat ttttaaatca aataaaagaa gatatacaat ctattgaaaa atacccatat
     6661 gaacatcatc actttcaaag tgatcgtaga atcatgatgg cgcttcagca tgttgcacaa
     6721 cataaaaata tcgcctttca atctttttat aatctcattg atactgtata taaagatcaa
     6781 cattttacaa tgtttgaaac ggacgctgaa ttattcggat attgttatgg tgttgctggt
     6841 acagtaggtg aagtattgac gccgatttta agtgatcatg aaacacatca gacatacgat
     6901 gtcgcaagaa gacttggtga atcgttgcaa ttgattaata tattaagaga tgtcggtgaa
     6961 gattttgaca atgaacggat atattttagt aagcaacgat taaagcaata tgaagttgat
     7021 attgctgaag tgtaccaaaa tggtgttaat aatcattata ttgacttatg ggaatattat
     7081 gcagctatcg cagaaaaaga ttttcaagat gttatggatc aaatcaaagt atttagtatt
     7141 gaagcacaac caatcataga attagcagca cgtatatata ttgaaatact ggacgaagtg
     7201 agacaggcta actatacatt acatgaacgt gtttttgtgg ataagaggaa aaaggcaaag
     7261 ttgtttcatg aaataaatag taaatatcat agaatatagg tggttgaata atgaagattg
     7321 cagtaattgg tgcaggtgtc acaggattag cagcggcagc ccgtattgct tctcaaggtc
     7381 atgaagtgac gatatttgaa aaaaataata atgtaggcgg gcgtatgaat caattaaaga
     7441 aagacggctt tacatttgat atgggtccca caattgtcat gatgccagat gtttataaag
     7501 atgtttttac agcgtgtggt aaaaattatg aagattatat tgaattgaga caattacgtt
     7561 atatttacga tgtgtatttt gaccacgatg atcgtataac ggtgcctaca gatttagctg
     7621 aattacagca aatgctagaa agtatagaac ctggttcaac gcatggtttt atgtcctttt
     7681 taacggatgt ttataaaaaa tatgaaattg cacgtcgcta tttcttagaa agaacgtatc
     7741 gcaaaccgag tgacttttat aatatgacgt cacttgtgca aggtgctaag ttaaaaacgt
     7801 taaatcatgc agatcagcta attgaacatt atattgataa cgaaaagata caaaagcttt
     7861 tagcgtttca aacgttatac ataggaattg atccaaaacg aggcccgtca ctatattcaa
     7921 ttattcctat gattgaaatg atgtttggtg tgcattttat taaaggcggt atgtatggca
     7981 tggctcaagg gctagcgcaa ttaaataaag acttaggcgt taatattgaa ctaaatgctg
     8041 aaattgagca aattattatt gatcctaaat tcaaacgggc cgatgcgata aaagtgaatg
     8101 gtgacataag aaaatttgat aaaattttat gtacggctga tttccctagt gttgcggaat
     8161 cattaatgcc agattttgca cctattaaaa agtatccacc acataaaatt gcagacttag
     8221 attactcttg ttcagcattt ttaatgtata tcggtataga tattgatgtg acagatcaag
     8281 tgagacttca taatgttatt ttttcagatg actttagagg caatattgaa gaaatatttg
     8341 agggacgttt atcatatgat ccttctattt atgtgtatgt accagcggtc gctgataaat
     8401 cacttgcgcc agaaggcaaa actggtattt atgtgctaat gccgacgccg gaacttaaaa
     8461 caggtagcgg aatcgattgg tcagatgaag ctttgacgca acaaataaag gaaattattt
     8521 atcgtaaatt agcaacgatt gaagtatttg aagatataaa atcgcatatt gtttcagaaa
     8581 caatctttac gccaaatgat tttgagcaaa cgtatcatgc gaaatttggt tcggcattcg
     8641 gtttaatgcc aactttagcg caaagtaatt attatcgtcc acaaaatgta tcgcgagatt
     8701 ataaagattt atattttgca ggtgcaagta cgcatccagg tgcaggcgtt cctattgtct
     8761 taacgagtgc gaaaataact gtagatgaaa tgattaaaga tattgagcgg ggcgtataag
     8821 ggagtagtct aagagaaaga tgtgagaaaa gtataagggg aacgtaaaag gcatagtgaa
     8881 ataagtatgt gcttaagaag ttttatttaa caatgaatga cgcctgttaa ggttcacgtt
     8941 tgaatacgta ccttgatggg cgttatactt tatataagca tatctaactg gttgcgcatg
     9001 cggatatgca tgacagttaa atcataacga ttgagtgcat attaatctga aaattcctaa
     9061 tattgacaat aatattatac ctacctaaaa tgaaaataaa ttcattcaat acaacgtgtg
     9121 tcataagttc tgttgaacat aacggtgaat ggcgatgtca ttgtaaccat ctaaatgaga
     9181 gtgatacaag ttgttaggat tatgatagtc gcccaggcta tgtttaatga tagaggagga
     9241 atttgatgat ctctaacaaa ttagcaaata tccctgatag ttatttcggg aaaacgatgg
     9301 gacgtaaaat cgaacatgga ccattgccat taattaatat ggcagttgga atccctgatg
     9361 ggcccacgcc acaaggtatt attgatcatt ttcaaaaagc actaactatt cctgaaaatc
     9421 aaaaatatgg tgcttttcat ggtaaagaag cgttcaagca agctattgta gatttttatc
     9481 aaagacaata taatgtgacg ttagataaag aagatgaagt atgcatttta tatggtacaa
     9541 aaaatggatt agtggcagta ccaacatgtg ttatcaatcc aggagactat gtattactac
     9601 ctgatccagg ctacacggat tatttagcag gtgtactttt agctgatggc aagccagtcc
     9661 cgcttaattt agaaccgcca cattatttgc cagattggtc caaagttgat tcacaaataa
     9721 tagataaaac aaaactcatt tatttaacgt atccaaataa tccaactgga tcgacagcta
     9781 cgaaagaagt ttttgatgaa gcgatagcta aatttaaagg tacagacact aaaatcgtgc
     9841 atgattttgc ttatggtgct ttcggtttcg acgctaaaaa tcctagtata cttgcttcgg
     9901 aaaatgggaa agatgttgcg attgaaattt attccttgtc taaaggttat aacatgtcag
     9961 gttttagagt cggttttgca gttggtaata aagatatgat tcaagctttg aaaaagtacc
    10021 aaacgcacac aaatgctggg atgtttggtg cattacaaga tgccgcaata tatgctttga
    10081 atcattatga tgatttttta gaagaacaaa gcaatgtatt taaaacacgt cgtgaccgat
    10141 tcgaagccat gcttgcaaaa gctgatttgc catttgttca tgctaaaggt ggcatatatg
    10201 tatggttgga aacaccgcct ggttatgaca gcgagcaatt tgagcaattt ttagtgcaag
    10261 agaagtcaat acttgttgct ccagggaaac catttggaga gaatggtaat cgttatgtga
    10321 gaatttcatt ggcgttagat gaccagaaat tagacgaagc tgcaataaga ttaacagaac
    10381 tagcatattt atatgaataa taaagttaag ggtgatgtta aaatgacaaa aattaaaatc
    10441 atgagtgtac gtgatgaaga tatgccttat atcaaggcgt gggcagagaa acatcatgtt
    10501 gaggtagata ttactaaaga agcattaact gatgacaatg ttgaaggtgt cgctggttat
    10561 gatggcttat cgctatcaca acaaatacca ttatcagagc atgtttataa acgattaaat
    10621 gaactaggta ttaaacaaat cgcacaacgt agtgctggat ttgatacata tgatttggag
    10681 ttagcaaaca agtataactt tattgtgtca aatgtgccgt catactctcc gaattcaatt
    10741 gcggagtttg ctgtgaacca ggctattaat gtggttcgtc attttaatca aattcaaacg
    10801 aaagtgcggg agcatgattt tagatgggaa cctactattt tatcgaaatc cattaaagat
    10861 ttaaaagtag ctgttattgg tacaggtcga attggccgtg tagtagccga tatatttgcc
    10921 aatggttatc aaagtgatgt ggtcgcatac gacccgtttc ctaatgctaa aattgcaacg
    10981 tatgtcgatt acaaagatac gattgaggaa gcggttgaag gtgctgatat cgtgacatta
    11041 catgtacctg caacgaaata taatcattat ctatttaatg ctgaattatt taaacatttt
    11101 aaaaagggcg cagtatttgt caattgtgcg agaggttctt tagtagatac taaggcgtta
    11161 ttagacgcat tagacaatgg tgtgattaaa ggtgcagcac ttgatacgta tgaatttgaa
    11221 cgcaaacttt tcccaagtga tcaaaggggc aaaacactga acgatccatt gttagaatcg
    11281 ttgattgaca gggaagatgt catattaaca ccacatattg cgttttatac tgaagctgca
    11341 gttaaaaatc taattgtcga tgcattagat gcaacattag atgtattgca gactggagat
    11401 actaggttac gagtaaatta aaaatcgaac tgatgagata atttggattg ttgggattct
    11461 catcagttcg attttttaat ttggtgttga tgacgttgaa tattgcctaa ttaaacgaca
    11521 tcgtaacctt gatcttcaat tgcgtctttc atttgagata cagcaacttt actgtcatca
    11581 tattgaacac ttacttgacc attttcaagg ttaacgtcag ctgaagtgac accgtcaata
    11641 ttatttaatg cagattcaac agcacttttg cagtgaccac agctcatacc ttcaacattt
    11701 aaaatttctt gtgacatagc attcacctcc ttttagagcc acttattatt tat
//
LOCUS       DAKTIO010000085        11637 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.085, whole genome shotgun sequence.
ACCESSION   DAKTIO010000085 DAKTIO010000000
VERSION     DAKTIO010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11637
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.085"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            433..636
                     /locus_tag="O0293_001650"
     CDS             433..636
                     /locus_tag="O0293_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280536.1"
                     /translation="MQLSHSVKVAISIYLALIFITFTSYLVIILYTSMTGHDVSHFVL
                     DSQHSHHGSLTQKHLSLPEISFK"
     gene            819..1754
                     /locus_tag="O0293_001651"
     CDS             819..1754
                     /locus_tag="O0293_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCY0280537.1"
                     /translation="MRYLANNHSALRLKGIILAIVGACLWGLGGTVSDFLFKYKNINV
                     DWYVTARLVVSGVFLLIMYKMMQPKRSIFSVFQDRRMLGKLLIFSILGMLVVQYAYMA
                     SINTGNAAIATLLQYIAPVYIIIWFVIRGVAKLTLFDVLAIIMTLLGTFLLLTNGSFS
                     NLVVNPASLFWGILAGVALAFYTIYPSDLLNRFGSILIVGWAMLISGVAMNLRHPIWH
                     IDITKWDISIILFLIFGIIGGTALAFYFFIDSLQYISAKETTLFGTVEPVVAVIASSL
                     WLHVAFKPFQIVGIILIMILILLLSLKRQPETLDE"
     gene            complement(1836..2804)
                     /locus_tag="O0293_001652"
     CDS             complement(1836..2804)
                     /locus_tag="O0293_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0280538.1"
                     /translation="MRKKWSTLAFGFLVAAYAHIRIKEKRSVKSYMLEQGIRLSRAKR
                     RFMYKEEAMKALEKMAPQTAGEYEGTNYQFKMPVKVDKHFGSTVYTVNDKQDKHQRVV
                     LYAHGGAWFQDPLKIHFEFIDELAETLNAKVIMPVYPKIPHQDYQATYVLFEKLYHDL
                     LNQVADSKQIVVMGDSAGGQIALSFAQLLKEKHIVQPGHIVLISPVLDATMQHPEIPD
                     YLKKDPMVGVDGSVFLAEQWAGDTPLDNYKVSPINGDLDGLGRITLTVGTKEVLYPDA
                     LNLSQLLSAKGIEHDFIPGYYQFHIYPVFPIPERRRFLYQVKNIIN"
     gene            complement(2937..3281)
                     /locus_tag="O0293_001653"
     CDS             complement(2937..3281)
                     /locus_tag="O0293_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCY0280539.1"
                     /translation="MVKQLNSVEAFREFIHQYPLAVVHVMRDQCSVCHAVLPQIEDLM
                     QSYPNVPLAVINQSQVEAIAGELNIFTVPVDLIFMNGKEMHRQGRFIDMQRFEHHLKQ
                     MNDSVNNDVDEH"
     gene            complement(3323..3733)
                     /locus_tag="O0293_001654"
     CDS             complement(3323..3733)
                     /locus_tag="O0293_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCY0280540.1"
                     /translation="MTTEGLLVAEKEIEVNGYDIDAMGVVSNIVYIRWFEDLRTAFIN
                     QHMNYSTMINQGISPILMKTEAEYKVPVTIHDKPVGRIYLVKASKMKWVFQFEIVSAH
                     GVHCIGTQTGGFYRLSDKKITSVPQVFQDILATK"
     gene            complement(4113..6179)
                     /locus_tag="O0293_001655"
     CDS             complement(4113..6179)
                     /locus_tag="O0293_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HCY0280541.1"
                     /translation="MFKKLFGQLQRIGKALMLPVAILPAAGILLAFGNAMHNEQLVEI
                     APWLKNDIIVMISSVMEAAGQVVFDNLPLLFAVGTALGLAGGDGVAALAALVGYLIMN
                     ATMGKVLHITIDDIFSYAKGAKELSQAAKEPAHALVLGIPTLQTGVFGGIIMGALAAW
                     CYNKFYNITLPPFLGFFAGKRFVPIVTSVVAIATGVLLSFAWPPIQDGLNSLSNFLLN
                     KNLTLTTFIFGIIERSLIPFGLHHIFYSPFWFEFGSYTNHAGELVRGDQRIWMAQLKD
                     GVPFTAGAFTTGKYPFMMFGLPAAAFAIYKNARPERKKVVGGLMLSAGLTAFLTGITE
                     PLEFSFLFVAPVLYGIHVLLAGTSFLVMHLLGVKIGMTFSGGFIDYILYGLLNWDRSH
                     ALLVIPVGIVYAIVYYFLFDFAIRKFKLKTPGREDEETEIRNSSVAKLPFDVLDAMGG
                     KENIKHLDACITRLRVEVVDKSKVDVAGIKALGASGVLEVGNNMQAIFGPKSDQIKHD
                     MAKIMSGEITKPSETTVTEEMSDEPVHVEALGTTDIYAPGIGQIIPLSEVPDQVFAGK
                     MMGDGVGFIPEKGEIVAPFDGTVKTIFPTKHAIGLESESGVEVLIHIGIDTVKLNGEG
                     FESLINVDEKVTQGQPLMKVNLAYLKAHAPSIVTPMIITNLENKELVIEDVQDADPGK
                     LIMTVK"
     gene            complement(6480..8219)
                     /locus_tag="O0293_001656"
     CDS             complement(6480..8219)
                     /locus_tag="O0293_001656"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HCY0280542.1"
                     /translation="MAKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQF
                     KFYHVRHEEVASLAAAGYTKLTGKIGVALSIGGPGLIHLLNGMYDAKMDNVPQLILSG
                     QTNSTALGTKAFQETNLQKLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVI
                     CPNDLLTEKIKDTTNKPVDTSRPTVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAK
                     DELREFIEMAKIPVIHSLPAKTILPDDHPYSIGNLGKIGTKTSYQTMQEADLLIMVGT
                     NYPYVDYLPKKNIKAIQIDTNPKNIGHRFNINVGIVGDSKIALHQLTENIKHVAERPF
                     LNKTLERKAVWDKWMEQDKNNNSKPLRPERLMASINKFIKDDAVISADVGTATVWSTR
                     YLNLGVNNKFIISSWLGTMGCGLPGAIASKIAYPNRQAIAIAGDGAFQMVMQDFATAV
                     QYDLPLTVFVLNNKQLAFIKYEQQAAGELEYAVDFSDMDHAKFAEAAGGKGYTIKSAS
                     EVDAIVEEALAQDVPTIVDVYVDPNAAPLPGKIVNEEALGYGKWAFRSITEDKHLDLD
                     QIPPISVAAKRFL"
     gene            complement(8266..8955)
                     /locus_tag="O0293_001657"
     CDS             complement(8266..8955)
                     /locus_tag="O0293_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCY0280543.1"
                     /translation="MNDYVQALLMILLTVVLYYFAKRLQQKYPNPFLNPALIASLGII
                     FVLLIFGISYNGYMKGGSWINHILNATVVCLAYPLYKNREKIKDNVSIIFASVLTGVM
                     LNFMLVFLTLKAFGYSKDVIVTLLPRSITAAVGIEVSHELGGTDTMTVLFIITTGLIG
                     SILGSMLLRFGRFESSIAKGLTYGNASHAFGTAKALEMDIESGAFSSIGMILTAVISS
                     VLIPVLILLFY"
     gene            complement(8948..9343)
                     /gene="cidA"
                     /locus_tag="O0293_001658"
     CDS             complement(8948..9343)
                     /gene="cidA"
                     /locus_tag="O0293_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like murein hydrolase modulator CidA"
                     /protein_id="HCY0280544.1"
                     /translation="MHKVQLIIKLLLQLGIIIVITYIGTEIQKIFHLPLAGSIVGLFL
                     FYLLLQFKIVPLTWVEDGANFLLKTMVFFFIPSVVGIMDVASEITLNYILFFAVIIIG
                     TCIVALSSGYIAEKMSVKHKHRKGVDAYE"
     gene            complement(9556..10434)
                     /locus_tag="O0293_001659"
     CDS             complement(9556..10434)
                     /locus_tag="O0293_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0280545.1"
                     /translation="MDIKHMKYFIEVVKQGGMTNASKSLYIAQPTISKAIKDIENEMG
                     TPLFDRSKRHLILTDAGQIFYEKSKEIVALYDYLPSEMERLNGLETGHINMGMSAVMN
                     MKILINILGAFHQQYPNVTYNLIENGGKTIEQQIINDEVDIGVTTLPVDHHIFDYTTL
                     DKEDLRLIVSREHRLAKYETVKLEDLAGEDFILFNKDFYLNDKIIENAKNVGFVPNTV
                     AQISQWHVIEDLVTNELGISILPTSISEQLNGDVKLLRIEDAHVHWELGVVWKKDKQL
                     SHATTKWIEFLKDRLG"
     gene            10601..10819
                     /locus_tag="O0293_001660"
     CDS             10601..10819
                     /locus_tag="O0293_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCY0280546.1"
                     /translation="MSFYEFMQSFLGDDTPLGELVDWINQDSNFPREVKSHSEILSYF
                     RTNPCPESISVPVIKRALSVFNQFTNVQ"
     gene            11208..>11637
                     /locus_tag="O0293_001661"
     CDS             11208..>11637
                     /locus_tag="O0293_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0280547.1"
                     /translation="MEYKKILIRLLIAFAVLFSADFTYQSVEQTHQSHAAVNYYSKNQ
                     CTWWAFKRRAQVGKPVSNRWGNAKNWYYNARKSKYATGRTPRKFAVMQSTAGYYGHVA
                     VVEQVYKNGSIKVSEYNFYRPLKYNTRVLSKKAARNFNYIY"
BASE COUNT         3630 a         2171 c         1740 g         4096 t
ORIGIN      
        1 atatttgtga aagattttta aaatggattg tttcaaaaac tacttatgtt gtcgttaata
       61 tttactaatt aactttttac tctatatttc aaacagttgt gtgacagttt tttgataact
      121 tttttacatc tgaaagtaag taatatttct aaaaactttt aatatttata cactttatct
      181 ttcgagctat ttattttttg tttttgtgct gtgtttataa aaccgcacac tatattcaaa
      241 aataacttat tacacatata tttgcaataa gataattaaa gtaggatatt atttttagtt
      301 ttctgatagg aatgatgata gtctataggt tgaatcttta ctttttttta aagctaaatt
      361 tacatcaact taacaatggt tggttatact gaagatgaaa atatttagta taacttagtg
      421 gaggcgataa aggtgcaatt gagtcattcc gttaaagttg caatttctat ctatttagca
      481 cttatcttta taacgttcac ttcttattta gtcattattt tatatacgag tatgactgga
      541 catgatgtat cacatttcgt gttagatagt cagcattctc atcatggatc tcttacgcaa
      601 aaacatttga gtcttcctga aatctcattt aaatagttta tcctctgtgt ttcaacattc
      661 atttcccata tcgattcatt tatctatcat ctagaccact acatcttaga tgattttttt
      721 attttctcat ttcactcttt cttaaaagtc gatataatga attaaatcat tatcatacac
      781 cgacatattt tatgttgttg gtgttaagtt ttaaaggggt gagatacttg gcgaataatc
      841 attcagcttt gcgtttaaaa ggaattatac ttgccattgt cggtgcttgt ttatggggat
      901 taggtggtac tgtttctgat ttcttgttca aatataagaa tattaatgtc gattggtacg
      961 tcactgctcg acttgtagtc agtggtgttt tcttacttat tatgtacaaa atgatgcaac
     1021 ccaaacgttc aatatttagc gtattccaag atcgacgtat gttaggcaaa ttacttatct
     1081 tcagtatact gggcatgtta gtagtacaat atgcttatat ggcatctatt aatacaggta
     1141 atgctgcgat tgcaacatta ctacaataca ttgcgccagt ttatattatt atttggtttg
     1201 tcataagagg cgttgcaaaa ctaacattat ttgatgtgct tgctattatc atgacactat
     1261 taggaacatt tttattatta acaaatggtt cattttctaa tttagtcgtc aatcctgcaa
     1321 gtttattctg gggtatttta gctggtgtag cactcgcttt ttacacaatt tatccttcag
     1381 acctacttaa ccgcttcggt tcgattctaa ttgtcgggtg ggcaatgctt atttctggtg
     1441 ttgcgatgaa tttacgccat ccaatttggc acattgatat cactaaatgg gacatatcaa
     1501 ttatattatt tttaatcttt ggtattatcg gtggtaccgc actcgcattt tatttcttta
     1561 tcgacagttt acaatacata tcagcgaaag aaacaacatt attcggaact gttgaacctg
     1621 tcgtagccgt tatcgcaagc agtctatggt tacatgtggc attcaaacca tttcaaatcg
     1681 taggcatcat tcttattatg attttaattt tattactatc acttaaaaga caacctgaaa
     1741 cattagatga ataagaaaac tctgataatc actttagcaa gtaactatta tttaacaacg
     1801 tagttacctt ataggtgata tcagagtttt ttattttagt taataatatt tttcacttgg
     1861 tataaaaagc gtcgtcgctc tggtatcgga aatactggat aaatatggaa ttggtaatat
     1921 ccaggtataa agtcatgttc gatacctttc gcactcaaca attgcgataa gttcaaagca
     1981 tctgggtaca gtacttcttt tgtaccaaca gttaatgtaa tacgacctaa accatctaag
     2041 tcaccattaa ttggtgatac tttgtagtta tctaaaggtg tgtcccctgc ccattgttca
     2101 gctaagaaca cactgccatc cacacctacc attgggtctt tctttaagta gtcaggaatt
     2161 tcaggatgct gcatcgttgc atctaaaact ggtgaaatta atacaatatg tcctggttgc
     2221 acaatatgtt tttcttttaa caattgagca aatgataaag caatttgacc gcccgcagag
     2281 tcacccatta caacgatttg tttagaatct gctacttgat tcaataaatc atggtacaac
     2341 ttttcaaaaa gcacatacgt cgcttgataa tcttgatgcg gaatcttcgg atatactggc
     2401 atgatgactt tagcattgag tgtttctgca agttcatcaa taaattcgaa atgaattttg
     2461 agtgggtctt ggaaccatgc gcctccatgt gcatataata caacgcgttg atgcttatct
     2521 tgtttatcgt taacggtata aacggttgaa ccgaagtgct tatccacttt tactggcatc
     2581 ttaaactgat aattggttcc ctcatattcg cctgctgtct gtggcgccat tttttctaat
     2641 gctttcatcg cttcttcttt atacataaaa cgacgcttag ctctagataa tcgtatacct
     2701 tgttctaaca tataactttt cacactgcgt ttttctttaa ttctaatatg tgcgtatgct
     2761 gcaactaaaa atccaaacgc aagtgtagac cattttttac gcatttgtcg tcactcctct
     2821 ttgatgattt ctactatagt tctactgtac catgctgatt ctaattacta ccaatattta
     2881 ctgggaaata agttatgatg tctacataat cttagcaatg ctaatcattt gcgatattaa
     2941 tgctcatcga catcgttatt tacactatca ttcatttgct taagatgatg ttcaaaacgt
     3001 tgcatatcga taaaacgccc ttgacgatgc atttcttttc cattcataaa aattaaatcc
     3061 acaggtacag tgaaaatatt taattctcca gcaatagctt ccacctgact ttgattaatc
     3121 acagctaatg gcacattggg atatgattgc atcaagtctt caatttgtgg taaaacggca
     3181 tgacacacgc tacactgatc gcgcatgaca tgtacaactg ctaacggata ttgatgaata
     3241 aattcacgga atgcttcgac actatttaat tgcttaacca tttcgaacac cttcttactt
     3301 tttatatttt aaaatgaagt cattattttg ttgctaaaat gtcttgaaac acttgtggca
     3361 cagaggttat cttcttatca ctcaatctgt aaaaaccgcc tgtctgtgta ccaatacaat
     3421 gcacgccatg tgcggacaca atttcaaact gaaacaccca tttcatcttg cttgctttaa
     3481 ctaagtaaat acgacctact ggtttgtcat gtattgtgac aggtacttta tactctgctt
     3541 ccgttttcat aagtataggt gaaatgcctt gattgatcat tgttgagtaa ttcatgtgct
     3601 gattaataaa cgctgttctc aaatcttcga accatctaat ataaacgata ttactaacga
     3661 cacccatcgc atcaatgtcg taaccattca cttcgatttc tttctctgca actaatagac
     3721 cttctgttgt cataatgaca aatctcctta tcttacaaat agatttaaaa tcatgttaat
     3781 ttatttttat tactttcatg ataaaaatac aagcaattga actttattac ggagaatcaa
     3841 acttttcaat gagactatta atttggctag tcgcgaagat gcatttttct aaaatgtgac
     3901 aaaaacataa gcaatgtttt ctatatctaa cactaatatt taacttatta tcatcaacac
     3961 acctgctata catcattgcg cactaaaaag gcagtagctt cattttaagc tactgccttt
     4021 tgtaacgtat tctctttcaa ctcgctactt cgcatacgca ctacgaatga ctaaaagttc
     4081 attcatttga tatgctgttt catttttaat cattatttga ctgtcataat tagcttacct
     4141 ggatcagcat cttgtacatc ttcaatgaca agttctttat tttcaagatt tgtaataatc
     4201 attggtgtaa cgatgcttgg tgcgtgtgct ttcaagtatg ctaaattcac tttcattaat
     4261 ggttgacctt gtgttacttt ttcatcaacg ttaatcagac tttcgaatcc ttcaccattc
     4321 agtttcactg tatcgatacc aatatgaata agtacttcga cgccactttc agattctaat
     4381 cctatcgcat gtttcgtagg gaagattgtt ttcactgtac catcaaacgg tgctacaatt
     4441 tcaccttttt cagggataaa gccaacacca tcacccatca ttttaccagc gaacacttga
     4501 tcaggtactt ctgataatgg aatgatttga ccgatacctg gtgcatagat gtctgttgtt
     4561 ccaagtgctt ctacgtgaac tggttcatct gacatttctt cagtcactgt cgtttcactt
     4621 ggtttcgtaa tttcaccact cataatcttg gccatatcat gtttaatttg atctgatttt
     4681 ggaccaaaga tagcttgcat attgtttcca acttctaata cacctgatgc gcctaaagct
     4741 ttaatacctg ctacatctac ttttgattta tcaaccactt ctacgcgtag acgtgtaata
     4801 catgcatcta aatgtttaat gttttctttt ccacccattg catctaagac atcaaatggt
     4861 aattttgcga cactagagtt acgaatttca gtttcttcat cttcacgacc tggtgttttc
     4921 aatttaaact tacgaattgc aaagtcgaat aagaagtaat acacgatagc atatacaata
     4981 ccgactggaa taactaataa tgcgtgtgaa cgatcccagt ttaataaacc atataaaata
     5041 taatctatga aaccacctga gaatgtcata ccaattttaa cgcctaataa atgcattact
     5101 aagaatgatg taccagctaa taatacgtga attccataaa gtactggtgc tacaaataag
     5161 aatgaaaatt ctaatggctc agtgatacca gttaaaaatg cagttaatcc tgctgataac
     5221 attaaaccac ccacgacttt tttacgttct ggtcgtgcat ttttataaat agcaaatgcc
     5281 gccgctggta aaccaaacat cataaatgga tatttaccag tagtaaatgc accagcagta
     5341 aatggtacgc catctttcaa ttgtgccatc caaatacgtt ggtcaccacg aaccaattca
     5401 cctgcgtgat ttgtataact tccgaattca aaccagaacg gtgaatagaa aatatgatgt
     5461 aaaccaaatg gaattaatga gcgttcaata ataccgaata taaacgttgt taatgttaaa
     5521 tttttattta ataagaaatt cgataaacta tttaatccat cttgaattgg tggccacgca
     5581 aagcttaaaa gcacacctgt tgcgattgct acgaccgatg tcacaatcgg tacaaatcgt
     5641 ttacctgcaa agaatcctaa aaatggtggt agtgtaatat tataaaattt gttgtaacac
     5701 catgcggcta aagcacccat gataatacca ccaaacacac ccgtttgtaa cgttggaata
     5761 cctaatacta aagcatgtgc tggttctttc gctgcttgac ttaattcttt tgcccctttg
     5821 gcatatgaga aaatgtcatc aattgtaatg tgcaacactt tccccattgt tgcattcata
     5881 attaagtaac ctactagcgc tgctaatgct gcaacaccgt ctcctcctgc taatccaagt
     5941 gctgtaccaa ctgcaaataa taatggcaag ttatcaaata caacttgtcc tgctgcttcc
     6001 atgaccgacg aaatcattac aatgatatcg ttttttaacc atggtgcaat ttctactaat
     6061 tgttcgttgt gcattgcgtt accaaacgct aataaaatac cagctgctgg taaaatcgca
     6121 acaggtaaca ttaatgcttt accgatacgt tgcaattgtc caaacaattt cttaaacata
     6181 taaacatccc ctatctcaat ttccgcttcc atgtacaaaa aaagcaggca tgaacaaaca
     6241 agactattct catagtgcgg ttgtttgctc atgcctgcat taccagtaac acgcaagtga
     6301 tatctaattc atagctatta tacatatttg taaaagcgtt ttcaacaact ttttcacaat
     6361 tttatcatca attcagtacg atcatttgtg ataaataaac aaaacggcaa ttcccatttt
     6421 gttgaaatag aaattaccgt ttttatagtt caattcaatt gtgataacct ttaaatcagt
     6481 tataagaaac gttttgctgc cactgaaatt ggtggaattt gatctaaatc taaatgttta
     6541 tcttcagtaa ttgatctaaa tgcccactta ccataaccaa gcgcttcttc atttacaatt
     6601 ttacctggta atggcgcagc attaggatca acatatacat ctacaatcgt tggtacatct
     6661 tgtgctaatg cctcttcgac tatagcatct acttcgctag cactcttaat tgtataacct
     6721 ttaccacctg ctgcctcagc aaattttgca tgatccatat cagaaaaatc aactgcatat
     6781 tctaattcac cagctgcttg ttgttcatat ttaataaatg ctaactgttt gttattaagt
     6841 acaaatacag ttaaaggtaa atcatattgt actgctgtag cgaagtcttg cattaccatt
     6901 tggaatgcac cgtcaccagc aattgcgatg gcttgtctat ttggatatgc aatttttgat
     6961 gcaattgcac ctggaagacc gcaacccatt gtacctaacc aacttgaaat gatgaacttg
     7021 ttatttacac caaggtttaa gtatcgagtt gaccaaactg ttgctgtacc tacatctgct
     7081 gaaatcactg catcatcttt aataaattta ttgattgatg ccattaatcg ttctggacgt
     7141 aatggtttac tattattatt tttatcttgt tccatccatt tatcccaaac cgctttacgt
     7201 tctaacgttt tgtttaagaa tggtctttca gcaacatgtt taatattttc agttaactga
     7261 tgcaacgcaa ttttactatc tccaacaatt cctacattaa tattgaaacg atgtccgata
     7321 tttttaggat ttgtgtcaat ttgaatggct ttaatatttt tcttaggtaa gtaatccaca
     7381 tatggatagt ttgtaccaac cataattaat aaatccgctt cctgcattgt ttgataagat
     7441 gttttggtac cgattttacc taagttaccg atactatatg gatgatcatc cggcaagatt
     7501 gttttagctg gtaatgaatg aatgacagga attttagcca tttcaataaa ttcacgtagc
     7561 tcatctttcg catgtttcgc acctacacca attaacatga caggcttttt acttttatta
     7621 attagtttaa ccgctttttt gatgtcttta tattttggtg atactactgt tggtcttgat
     7681 gtatctactg gtttattcgt tgtatcttta attttttcag ttaataagtc gttaggacaa
     7741 ataacaacag ctacaccttt ttgttcatat gccgtacgaa ttgcttcgtt aacgatttca
     7801 aacacattgt cacctttttc aatttggtga ttataaacgg ctacatcttc acataatttt
     7861 tgtaaatttg tttcttggaa tgctttcgtt ccaagtgctg tactattcgt ttgtccagat
     7921 aatattaatt gcggtacatt atccattttg gcatcataca taccatttaa taaatgaatt
     7981 aaaccagggc caccgatact taatgccaca ccgattttac cagttaattt tgtgtaacca
     8041 gcagccgcta agcttgctac ttcttcatga cgtacatgat aaaatttaaa ttgatctctc
     8101 actgtacgta aactatcgac tactgcgtcg attgagtctc ctggaatacc atacaagtga
     8161 tctatatccc atgcttgtaa tgctttaact aatgcttcat ttgcttttat ttttgccatt
     8221 attactaata gcctcccttt ctgtctatca ttttaaatat ctaaattaat agaataataa
     8281 aattagaaca ggtattaaca ctgaactaat aactgcagtt aaaatcatcc caattgaact
     8341 aaatgcaccg gattcaatat ccatttctag tgctttagct gtgccaaatg catgtgacgc
     8401 attcccatac gttaatcctt tggcgataga agattcaaat cttccaaatc ttaataacat
     8461 cgaacctaaa atactaccga ttaaacccgt tgtgataata aaaagtacgg tcatcgtatc
     8521 tgtaccacct agttcatgtg acacttcgat acctactgcg gctgttatag atcggggcaa
     8581 taacgttaca atgacgtctt tagaatagcc aaatgcttta agtgttaaga acactaacat
     8641 gaaattcagc atgacgccag ttaatacact tgcaaaaatg atagagacat tgtctttaat
     8701 tttctctcta tttttataaa gtgggtacgc taaacatacg accgttgcgt ttaaaatatg
     8761 gttgatccaa ctgccacctt tcatataccc gttataacta attccaaaga taagtaagac
     8821 aaaaataatt cctaaagatg caattaatgc tggattcaaa aatgggttcg gatatttttg
     8881 ttgtaacctt ttagcgaaat aatataaaac gacagtcaac aaaatcatta ataaggcttg
     8941 cacgtaatca ttcataagcg tctacacctt tacgatgttt atgtttaaca gacatttttt
     9001 cagcaatata acctgaagat aatgcaacga tacatgttcc tatgataatg actgcgaaaa
     9061 agagtatata atttagcgta atttcggaag caacatccat aatacctaca actgacggta
     9121 tgaagaaaaa gaccatcgtc tttaataaaa agtttgcacc gtcttctacc caagttagcg
     9181 gtacaatctt aaattgtaat agtaaataaa ataaaaatag accaacaata ctgccggcta
     9241 agggaagatg aaaaatcttt tgaatttctg tgccaatata agtaatcaca atgatgattc
     9301 ctagttgtag tagtagtttg attattaatt ggactttgtg catggcgcca tccctttcta
     9361 aatatgtcta aattgttaca ataactatta taaagatggc gtacacaata taaaaatact
     9421 ctttttttat agtttgtatt actattttct atggaaacgc tctctaaatt tcacttcatt
     9481 ttcacaaaaa aacactcatt atttcaatga cccacgtaat gatgcgcctt aaataatgag
     9541 tgctttatta atatgttagc ctaaacggtc tttcaaaaat tctatccatt tcgttgtggc
     9601 atgacttaat tgtttatcct tcttccaaac aacacctaat tcccaatgta catgagcatc
     9661 ttcaatgcgt agcagcttca catctccatt tagttgctct gaaattgatg ttggtaaaat
     9721 actaatacct aattcattcg taactaaatc ttctataaca tgccattgtg aaatttgcgc
     9781 tacagtattc ggaacaaagc caacgttttt agcattttca ataattttat cattcaagta
     9841 aaagtcttta ttaaataaaa tgaagtcttc acctgctaaa tcttcgagtt taacagtttc
     9901 atattttgcg agtcgatgct ctctgctcac gataagtcgc aaatcttcct tatctagggt
     9961 agtataatcg aaaatatgat gatcgactgg caaagtggtc acgcctatat ctacttcatc
    10021 attgataatt tgctgttcaa ttgttttacc gccattttct atcaaattat atgtaacatt
    10081 tggatattgt tgatggaatg caccaagaat attgataaga atcttcatat tcatgactgc
    10141 cgacatgccc atgtttatat gtcctgtttc cagtccattc aagcgttcca tttcagatgg
    10201 taaataatca tacagtgcaa caatttcttt acttttctca taaaaaattt gacctgcatc
    10261 ggtaagaatt aaatgtcttt tacttctatc aaataagggc gtgcccattt cattttcaat
    10321 atctttaatt gctttactaa ttgtaggttg tgcaatatat aatgatttgg aagcattagt
    10381 catgcctcct tgcttaacga cttcaataaa atatttcata tgtttgatat ccactaacat
    10441 catcctaact atttcaacac gtttatttca ttatacacaa cttcttaaac attcaataaa
    10501 tttagattta taaaattgct ttataaaaag ttttatgtaa aatacacatt cttttatggt
    10561 attatattaa tggctcaagt atattaatcg aggtgaggta ttgagctttt acgaatttat
    10621 gcaaagtttt cttggtgatg acacaccatt aggtgaattg gttgattgga ttaatcaaga
    10681 tagcaatttc cctagagaag tgaagagcca tagtgaaata ttgtcatatt ttcgaacaaa
    10741 tccatgtcct gaaagcatct cagtacctgt aattaaacgg gcactatcag ttttcaacca
    10801 attcacaaat gtacaatgat acatgaatac atggagtcac atcatctcat gttagcgctc
    10861 tgattgcagg gcgctatttt taatgttatt gctacattta gtatattgtg attgctattt
    10921 tctttcagtt atagaaccat gtaccttttt tttaaatcat tttatatttt cccactaata
    10981 tccgctgtta atcaatcctg acatccttgt atcactatga caattaattg ttaaatacat
    11041 gaatttctac attttatgaa aaaatccatt tttattacaa ttcaacactt tatatgacaa
    11101 cttcattaca gttactttta ttgttgattg cttacattgt tttctaaaaa aaatttgtta
    11161 tcataattaa cgttgaataa agaaaaaaat taacttggga gataaaaatg gaatataaaa
    11221 agatactaat tcgtttatta attgcttttg cagtactttt ctcagcagat ttcacttatc
    11281 aatccgtcga acaaacgcat caatcgcatg ccgcagttaa ttattatagt aaaaaccaat
    11341 gtacatggtg ggcatttaaa cgtcgcgcac aagtcggtaa acctgtttct aatagatggg
    11401 gcaatgctaa aaattggtat tacaatgcac gtaaatcaaa atatgcgact ggtcgtacac
    11461 caagaaaatt tgctgtcatg caatcaactg caggatatta tggacatgtc gcagttgttg
    11521 aacaagtata taaaaacggt agtattaaag tttcagaata caacttttat cgcccattaa
    11581 aatacaatac acgtgtacta agcaaaaagg cagcacgtaa ctttaactat atttact
//
LOCUS       DAKTIO010000086        11373 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.086, whole genome shotgun sequence.
ACCESSION   DAKTIO010000086 DAKTIO010000000
VERSION     DAKTIO010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11373
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.086"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            128..766
                     /locus_tag="O0293_001662"
     CDS             128..766
                     /locus_tag="O0293_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HCY0280548.1"
                     /translation="MDDLNKKYLIDLHQHQNSSIEVLREFAEVNEVPIVDRLTLDLIK
                     QLIRMNNVKNILEIGTAIGYSSMQFASISDDIHVTTIERNETMIQYAKQNLATYHFEN
                     QVRIIEGNALEQFENVNDKVYDMIFIDAAKAQSKKFFEIYTPLLKHQGLVITDNVLYH
                     GFVSDIGIVRSRNVRQMVKKVQDYNEWLIKQPGYTTNFLNIDDGLAISIKGE"
     gene            769..1692
                     /locus_tag="O0293_001663"
     CDS             769..1692
                     /locus_tag="O0293_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCY0280549.1"
                     /translation="MTELLVTPKSLSHMETLIDLGADAFVIGEQKFGLRLPGEFNRQQ
                     MTEAVALAHKNDKKVYAAVNGLFHNYHLDAVEDYINFLHEIRVDRIIFGDPAVVMYVK
                     AQENPIPLHWNAETLVTNHFQCNYWGKRGASRAVLARELNLDEIINIKENSNVEIEVQ
                     VQGMTCMFQSKRMLLGNYYTFQDRQMKIERRNDEQSLLLYDEERQNNYPVYEDYNGTH
                     IMSPNDICLIEELAPFFEAGIDSFKIDGILQTEEYINVVTEQYRQAIDLYNEDPEIYE
                     DEKFMLMDPIEEIQPDHRPFDEGFLYKQTVY"
     gene            1704..2972
                     /locus_tag="O0293_001664"
     CDS             1704..2972
                     /locus_tag="O0293_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCY0280550.1"
                     /translation="MKTIEEIKSTPKTVMKKPELLAPAGNLEKLKIAVHYGADAVFLG
                     GQEYGLRSNADNFTMEEIAEGVEFANRYGAKIYVTTNIIAHDENIEGLESYLRNLEKT
                     GATGIIVADPLIIETCKEVAPKLEIHLSTQQSLSNYKAVEYWKEEGLDRVVLARETGA
                     MEMREMKEKVDIEIEAFIHGAMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYELLE
                     VDDNGELDVFYNQGEVTPFAMSPKDLKLIESIPQMMDIGVDSLKIEGRMKSIHYIATV
                     VSVYRKVIDAYAADPDNFKINPEWLIELDKCANRDTAPAFFEGTPGYEEQMFGQQQSK
                     KSPFDFCGLVLDYNEDTKIATIQQRNNFKPGQEIEFFGPEIETFTQVVEAIYDEEGNS
                     LDAARHPLQIVQIKVDRPIYPNNMMRKEIG"
     gene            2972..3595
                     /gene="udk"
                     /locus_tag="O0293_001665"
     CDS             2972..3595
                     /gene="udk"
                     /locus_tag="O0293_001665"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCY0280551.1"
                     /translation="MKATTIIGIAGGSGSGKTTVTNEIMKNLEGHSVALLAQDYYYKD
                     QKHLTFDERLETNYDHPFAFDNDLLIENLKDLKNGKAVEVPTYDYASHTRSDITIDFK
                     PKDVIIVEGIFALENKVLRDMMDVKIYVDTDADLRILRRLTRDTKERGRSMDSVINQY
                     LSVVRPMHDQFIEPTKKYADIIIPEGGSNKVAIDIMTTKIQSLVSKQ"
     gene            3623..4099
                     /gene="greA"
                     /locus_tag="O0293_001666"
     CDS             3623..4099
                     /gene="greA"
                     /locus_tag="O0293_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCY0280552.1"
                     /translation="MENQKQYPMTQEGFEKLERELEELKTVKRPEVVEKIKVARSFGD
                     LSENSEYDAAKDEQGFIEQDIQRIEHMLRNALIIEDTGDNNVVKIGKTVTFVELPGDE
                     EESYQIVGSAESDAFNGKISNESPMAKALIGKGLDDEVRVPLPNGGEMNVKIVNIQ"
     gene            4425..5159
                     /gene="pxpB"
                     /locus_tag="O0293_001667"
     CDS             4425..5159
                     /gene="pxpB"
                     /locus_tag="O0293_001667"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCY0280553.1"
                     /translation="MDVRFINEQTIMIYFENKISEETYRNVTAMVRWIREKEILEIQD
                     IVPSYRAVLIYFDEQAITSSKLIENLELNKFNEKNVHAVNQTNRIIKIPVQYGGTYGP
                     DIEEVAKHNRITVEQVIEKHTSKPYLIYMLGFMPGFPYLGGLDEQLHTPRRNQPRLKI
                     HAGSVGIANNQTGLYPSDSPGGWQIIGRTPLKVFSSEREPMSMYEAGEWIQFYAIDEQ
                     KFIQIERDISDGNFNVDDWVVIENVN"
     gene            5149..6159
                     /locus_tag="O0293_001668"
     CDS             5149..6159
                     /locus_tag="O0293_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HCY0280554.1"
                     /translation="MSIKILQPGLFSTVQDLGRIGYEHIGFSGAGAMDQFSFKVAQSL
                     INNDGPAIEYTLIGPTIQFNTQNTFVITGGSINASLNNKTISMNSVILAEKGDILKIG
                     AITKGARGYLTFGHSINVPSIAESYATHTRSSIGGFKGRKLLADDVITVKINNDFKEN
                     IGKTIHLQDDLLPENNIIHILQGPQFEAFSEEARAKIVNHPYLITEQSDRMGYRLEGD
                     SVAPINQADIISEPVALGSVQVPNDGQPIILLNDKQTIGGYTKIATVCKFDLPKLAQM
                     KPQDTIQFKWISFQEAVDKNREQMSLFNEILKSHQKTPIFDTSSLRHTSKKLATILKG
                     DL"
     gene            6161..6610
                     /locus_tag="O0293_001669"
     CDS             6161..6610
                     /locus_tag="O0293_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HCY0280555.1"
                     /translation="MNIEKIEQIIKLVKENDVKKFKYKNFEDEIEIDFTDSNHLAAHS
                     NQSNQSMNNNDLTASKANDNSDVSTNDYHDIKSPMIGTFFLQDSKELTEPIVNVGDKV
                     NKGDIIGYVEAMKVLNEVTTDVAGEITEIVADHGTNVEYDQVLVRIK"
     gene            6624..7985
                     /locus_tag="O0293_001670"
     CDS             6624..7985
                     /locus_tag="O0293_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCY0280556.1"
                     /translation="MLRCLIANRGEIAVRIIRACREYGIETVAVYAKGDEQSLHVHLA
                     DQAICIGEANALDSYLNIDRIISAAQITGANAIHPGYGFLSESTKFAQTVEEQGIAFI
                     GPTKKTMEMMGDKITARQTVHHAGVPVIPGSNGAVNHVSEIENLAKDIGYPVVIKAAS
                     GGGGKGIRIVKKAEDLEKAFKEAKSEGKKYFDDDRVYVEAFIPVAKHVEVQVMGDGQD
                     NYVHLGERDCSVQRKNQKLIEESPCAALTEERRQQICNDAVKVARAANYRSAGTIEFL
                     VTDTAHYFIEMNARIQVEHTVTEMRAERDLVAAQLYLLEHNHLPFSQSDIQFNGHVIE
                     ARINAENPEKKFQPTPGKVTALHLPQGFNVRVDSLLYHGYQVSPYYDSLVAKVIVKSH
                     DRASAIDKLKVTLDEMVIDGFSTTADFLYAVLNYPLYRDGDAKDVDIKFLEKHQIVKG
                     VEL"
     gene            7985..8737
                     /gene="pxpA"
                     /locus_tag="O0293_001671"
     CDS             7985..8737
                     /gene="pxpA"
                     /locus_tag="O0293_001671"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCY0280557.1"
                     /translation="MRVDLNCDLGEAFGNYSFGGDHQIIPLITSANVACGFHAGDENV
                     MNETVKLAKAHNVAVGAHPGLPDLKGFGRRNIDISNDEIYNLMIYQLGALQGFCRIHQ
                     VKINHVKPHGALYQMGAKDREIANVIAQAVYDFDPSLVLVGLANSYLISEAKNVGLIT
                     ASEVFADRRYEDDGQLVSRKESDAVITDTDEALKQVLKMVKENKVISKNNKEVTLQAD
                     TICVHGDGEHALLFVSKIREILMKEGIDIQSL"
     gene            8750..9979
                     /locus_tag="O0293_001672"
     CDS             8750..9979
                     /locus_tag="O0293_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HCY0280558.1"
                     /translation="MGRNLKLKKESDFEFTKNHKRLLLGSVFLMATSAIGPAFLTQTA
                     VFTSQFFASFAFAILLSIIIDIGAQINIWRILVVTGLRGQEISNKVVPGLGTVISILI
                     AFGGLAFNIGNIAGAGLGLNAIFGLDVKWGAAITAIFAILIFVSKSGQKIMDVVSMIL
                     GIVMILVVAYVMFVSNPPYGDAFVHTFAPEHPMKLVLPIITLVGGTVGGYITFAGAHR
                     ILDSGIKGKQYLPFVNQSAIAGILTTGIMRTLLFLAVLGVVVTGVTLSSENPPASVFE
                     HAIGPIGKNIFGIVLFAAAMSSVIGSAYTSATFLKTLHKSLNERSNLIVIVFIVISTM
                     IFLFIGKPISLLIIAGAINGWILPITLGAILIASKKKSIVGDYKHPNWMFIFGIVAVL
                     VTILTGIFSFKEVLQLF"
     gene            10268..>11373
                     /locus_tag="O0293_001673"
     CDS             10268..>11373
                     /locus_tag="O0293_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HCY0280559.1"
                     /translation="MGYRTLKSIFHEHNESKMKEEYIKRFNSLASFNTNINIIPMENG
                     KKVNDLEYPLFFMVTKNLSKKQELISINSRKIDRALNSLPYAAREQYFNDLLIDELQS
                     TNEIENVFSTKQEIAHALNNQASEFLKFRGLVDQYKEIELNKKIKVDNVRDIRAIYDK
                     LVSNEINEQDKLDGELFRKNFVGVHDGSTNKYIHVGLQPETKIVEFIGEMLTFLKYFD
                     APQPFKIMASHYLFEYIHPFYDGNGRVGRFIIAKLLSDYYDNYTALTFSYVINRNKSK
                     YYKAFMTASNHLNCGDLTEFIDTMLELLIAGQERILDELIPKMDATEKLTLYLTSHYK
                     QIDYEFLYLLSMDKLFGNKRNRLTLIDLENILGVG"
BASE COUNT         4112 a         1528 c         2209 g         3524 t
ORIGIN      
        1 gatagctttg cgtctaacac gaaatcagtc atttaattgg ttggcaaaat tataatcaat
       61 tgtgaaggct gatttatttt attgtcataa tcatattgac ttattttaca taaaggaagt
      121 ttaaaacatg gatgacctaa ataaaaaata tttaatagat ttacatcaac atcaaaatag
      181 ttcaatcgaa gttttgcgtg aatttgccga ggtaaatgaa gtgccaattg tagatcgttt
      241 aacattagat ttaattaagc aattaattcg tatgaataat gttaaaaata ttttagaaat
      301 tggtacagca atcggctata gttctatgca attcgcttct atatctgatg acattcatgt
      361 cacaacgata gagcgtaatg aaacgatgat tcaatatgct aaacaaaatt tagctactta
      421 tcattttgaa aaccaggttc gaattattga aggtaatgct ttagagcaat ttgaaaatgt
      481 aaatgacaaa gtttatgata tgatatttat tgatgcagca aaagcgcaat caaagaaatt
      541 ttttgaaata tatacaccac ttttaaagca ccaaggtctc gtaattacag ataatgtttt
      601 atatcacggt tttgtatcgg atattgggat tgttagatcg agaaatgtaa gacaaatggt
      661 taaaaaggtt caagattata atgagtggtt aataaagcaa ccaggatata caacgaattt
      721 tttaaatata gacgatggat tagcgatttc aattaaagga gaatgactat gacagaatta
      781 cttgtcacac ccaagtcatt aagccatatg gaaacactta ttgatttagg tgcagatgct
      841 tttgtaattg gcgagcaaaa gtttggttta agacttccag gggaattcaa tagacaacaa
      901 atgacagagg ctgtcgcatt agctcacaaa aacgataaaa aagtatatgc ggcagtgaat
      961 ggattatttc ataattatca tttagatgct gtagaagatt atattaattt tttacatgaa
     1021 atacgagttg acagaattat tttcggggat ccagcagtag taatgtatgt gaaggcgcaa
     1081 gaaaatccaa taccacttca ttggaatgca gagacgttag taactaatca ttttcaatgt
     1141 aattactggg gtaaaagagg cgcatcaaga gctgtcttag cacgtgagct gaatttggat
     1201 gaaattatta atataaaaga aaattcaaat gtggaaattg aggtgcaagt tcaaggaatg
     1261 acttgtatgt ttcaatcaaa gcgtatgcta ctaggcaatt attatacgtt ccaagatcgc
     1321 caaatgaaaa ttgaacgtcg caatgatgaa caatctttat tactttatga tgaagaaaga
     1381 caaaataatt acccagttta cgaagattac aatggtacgc acattatgtc accaaacgac
     1441 atttgtttaa ttgaagaatt agcaccattt tttgaggcgg gtatagattc atttaaaatc
     1501 gatggtattc tacaaacgga agaatatatt aatgtggtaa cggaacagta tcgtcaagcg
     1561 atagatttgt acaatgaaga tcctgaaatc tatgaagatg agaaatttat gttgatggat
     1621 ccaattgaag aaattcaacc tgatcatcga ccatttgacg aaggtttctt atataaacaa
     1681 acagtatatt aaggaggtta atcatgaaga caatagaaga gattaaatca actcctaaaa
     1741 cagttatgaa gaaaccagaa ttattagcac ctgctggaaa cttagaaaag ttaaaaatag
     1801 cagtacatta tggcgctgat gccgtatttt taggtggtca agaatatgga ttacgttcaa
     1861 atgctgataa tttcacgatg gaagaaatag ctgaaggtgt tgaatttgcg aaccgttacg
     1921 gtgccaaaat ttatgttacg acaaatatta ttgcacatga tgagaatatt gaaggtctag
     1981 aatcatattt gcgtaatttg gaaaagactg gtgcgacagg tatcattgtt gcagatcctt
     2041 taattatcga aacatgtaaa gaagttgcgc caaaacttga aattcattta tctactcaac
     2101 aatcactttc taattacaaa gctgtagaat attggaaaga agaaggattg gatcgtgttg
     2161 tattagcacg tgagaccggc gcgatggaaa tgcgtgaaat gaaggaaaaa gtagatattg
     2221 aaatcgaagc atttattcat ggtgctatgt gtatcgccta ttcaggtaga tgtacattaa
     2281 gtaatcatat gactgcaagg gattccaaca gaggcggttg ctgtcaaagt tgccgttggg
     2341 attatgaatt attagaagtt gatgataatg gtgaacttga tgttttttat aatcaaggtg
     2401 aagttacacc gtttgcgatg agtcctaaag atttaaaatt aatcgaatca attcctcaaa
     2461 tgatggatat tggtgtggac tcattaaaaa ttgaaggacg tatgaagtca attcattata
     2521 ttgcaacagt tgtctcagta tatcgtaaag tcattgatgc gtatgcggca gatcctgaca
     2581 actttaagat taatccggaa tggttaatag agttagataa atgtgcaaat agagacactg
     2641 caccagcatt ctttgaggga acacctggtt atgaagaaca gatgtttggt caacaacaat
     2701 ctaaaaaatc accatttgat ttttgtggtt tggtattaga ctataatgaa gatacaaaaa
     2761 ttgcgactat tcaacaacga aataacttta aaccaggtca agaaatagaa ttctttggtc
     2821 cagaaattga aacatttaca caagtagtag aagcaattta tgatgaagaa ggtaacagct
     2881 tagacgcggc gcgccatcca ttacaaatcg ttcaaattaa agtggatcgc ccgatatatc
     2941 caaacaacat gatgagaaag gaaattggct aatgaaagct actacaatca ttggcatagc
     3001 tggtggatct ggctcaggaa aaacaactgt aactaacgaa attatgaaaa acttagaagg
     3061 tcatagtgtc gctttacttg ctcaagatta ctattataaa gatcaaaagc acttgacttt
     3121 cgacgagcgc ctagaaacca attatgacca tccatttgca ttcgataatg atttattaat
     3181 tgaaaatctt aaagacttga aaaatggtaa agcagtagaa gtaccgacat atgattatgc
     3241 tagtcataca agaagtgaca ttaccattga ttttaaacct aaagatgtta ttatcgtaga
     3301 aggcattttc gctttagaaa ataaggtatt acgtgatatg atggatgtta aaatatatgt
     3361 tgatacagat gcagacttga gaatattacg ccgtttaaca cgagatacta aagagcgtgg
     3421 gcgttcaatg gactctgtta tcaatcaata tttaagtgtt gttagaccta tgcatgacca
     3481 atttattgaa ccgactaaga aatatgctga tataattatt cctgaaggtg ggagcaataa
     3541 agttgcaata gatattatga caacaaaaat tcagtcttta gttagcaagc aatagtataa
     3601 ttcagtaaag gaagatgaca atatggaaaa tcaaaagcaa tatccaatga ctcaagaagg
     3661 ttttgaaaaa ttagagcgtg aacttgaaga attaaaaaca gttaagcgtc ctgaagttgt
     3721 agagaaaatt aaagttgcac gttcatttgg tgacttatca gagaactctg agtatgatgc
     3781 agcaaaagat gaacaaggat tcatcgaaca agatattcaa agaattgagc atatgttaag
     3841 aaatgcatta atcattgaag atactggaga taacaacgtt gttaaaattg gtaaaacagt
     3901 aacgtttgta gaattaccag gtgatgaaga ggaaagttat caaatcgttg gttcagctga
     3961 atcagatgca tttaatggta agatttcaaa tgaatcacca atggctaaag cgttaattgg
     4021 taaaggttta gatgatgaag ttcgtgttcc actacctaat ggtggcgaaa tgaacgtaaa
     4081 aattgttaat atccaataaa tgatcaaata gattatttaa ttgcgcgatt gaagtgtgtt
     4141 aatgatactt cggtcgtgct tttttatatt caaaatcaaa aaatcggaca gatgaaaagt
     4201 aaaaattttc catcagaccg aatgatagca gaacctaaat tagtgtaaga gttacaatat
     4261 taactactcc attttaaaaa agcaaaatat ttccgagaaa acaaattgat tatcatttgt
     4321 tattgtatgc gatttgaatt acttattaaa caacttgtgc ttctcaatga tacaatgaaa
     4381 aggtattcaa tactggttta ttgcaataca aagggggatt taaagtggat gtaagattta
     4441 ttaacgagca gacaattatg atttattttg aaaataaaat atccgaagag acatatcgta
     4501 atgtcacagc aatggtacgg tggatacgag aaaaagaaat attagaaatt caagatattg
     4561 tcccttcgta tcgagcagtt ctaatatatt ttgatgaaca agctataaca tcttctaaat
     4621 taattgaaaa tttagaactt aacaaattta atgaaaaaaa tgttcatgct gtgaatcaaa
     4681 ccaatcgtat tattaaaata cctgttcaat atggtggtac atatggacca gatattgaag
     4741 aagtagcaaa gcataatcga ataactgttg agcaagttat tgaaaaacat acaagtaaac
     4801 cttatttaat atatatgcta ggatttatgc caggatttcc atacttaggc ggacttgatg
     4861 aacaactaca tacacctaga cgtaatcagc caaggttaaa aattcatgca ggttctgtag
     4921 gtattgcgaa taatcaaaca ggactatatc catcagattc tcctggcgga tggcaaatta
     4981 ttggacgcac accgttaaaa gtatttagca gcgagcgtga accaatgtca atgtacgaag
     5041 caggagaatg gatacaattt tatgcaatag atgaacagaa atttatacaa atcgaaagag
     5101 atatatcaga tggcaacttt aatgttgatg actgggtggt gattgaaaat gtcaattaag
     5161 attttacaac caggtctctt ttcaacggta caagatctag gaagaatagg ttatgaacat
     5221 attggatttt caggcgcagg tgccatggat caatttagtt ttaaggttgc gcagtcatta
     5281 attaacaatg atggtccagc gattgaatat actttgattg gtcctaccat tcaatttaat
     5341 acgcaaaata catttgttat aaccggtggt agtattaatg cttcgctaaa taataaaact
     5401 atatcaatga attctgtcat attagctgag aaaggtgaca ttttaaaaat aggtgctata
     5461 accaaaggtg cacgcggtta tcttactttt ggtcattcta tcaacgtacc ttctattgct
     5521 gaaagttatg caacacatac ccgaagcagt ataggaggat tcaaaggtag aaagttatta
     5581 gctgatgatg taataacagt gaaaataaat aatgacttta aagaaaacat tggaaagacg
     5641 attcatttac aggacgattt attgccagaa aataatatta tacacattct tcaaggacct
     5701 caattcgagg cattttctga agaggctaga gcgaaaattg taaatcatcc atatttaatt
     5761 actgaacaat cagaccgtat gggctatcgt ttggaaggtg acagcgttgc accaattaat
     5821 caagcagata tcatttctga accggttgct cttggtagtg ttcaggtacc aaatgatggt
     5881 caacctatta ttctgcttaa tgataaacaa acgataggtg gttataccaa aattgcaact
     5941 gtatgcaaat ttgatctgcc aaagttagca cagatgaaac cacaagatac aattcaattt
     6001 aaatggatat cattccaaga agctgtagat aagaatcggg aacaaatgtc tctgtttaat
     6061 gaaattctta agagtcatca aaagactcca atttttgata catctagctt gcgacatact
     6121 tcgaaaaaat tagcaacaat tttaaaaggg gatttgtaaa atgaatattg aaaaaatcga
     6181 acaaataatc aaattagtga aggaaaatga tgttaagaaa tttaaatata aaaattttga
     6241 agatgaaata gaaattgact tcactgactc gaatcatttg gctgcacaca gtaatcaatc
     6301 aaatcaaagt atgaacaata atgatttgac agcttcaaaa gcgaatgata actccgatgt
     6361 ttcgacaaat gattatcatg acattaaatc accaatgata ggtacattct ttttacaaga
     6421 tagtaaagaa ttaactgaac caattgtgaa tgtcggtgac aaagttaaca agggagatat
     6481 tataggatat gttgaagcga tgaaagtatt aaacgaggta acaacagatg ttgctggaga
     6541 aattactgaa atagtagctg atcatggcac aaatgttgaa tacgaccaag ttttggtacg
     6601 tattaagtaa agggggtaag taaatgcttc gttgtttaat tgcgaacaga ggtgaaattg
     6661 ctgtaaggat tataagagct tgcagagaat atgggattga aactgtagca gtttatgcaa
     6721 aaggggacga gcaaagcttg catgtacatt tagcagatca agctatatgt attggggaag
     6781 ctaatgcttt agatagttat ttaaatattg accgcatcat atctgctgca caaatcactg
     6841 gtgctaatgc aattcaccca ggatatggct ttttatcaga atcaacaaaa tttgctcaaa
     6901 ccgttgagga acaaggcata gcgtttatag ggcctactaa aaaaacgatg gaaatgatgg
     6961 gggataaaat aacagcaaga caaacagttc atcatgcagg tgttcctgtg attccaggat
     7021 caaatggtgc tgtgaatcat gtgtctgaaa ttgagaatct tgccaaagac atcggttatc
     7081 cagttgttat caaagctgcc agtggcggtg gtggtaaagg tatacgtatt gtaaagaaag
     7141 ctgaagattt agaaaaggca tttaaagaag ctaaaagtga aggaaaaaaa tactttgatg
     7201 atgatcgtgt ttatgttgag gcttttatac ctgttgcaaa acatgttgaa gttcaagtta
     7261 tgggagatgg gcaggataat tatgtacatt taggggaacg tgattgttct gtacaacgta
     7321 aaaatcaaaa attaatcgaa gaatctcctt gtgcagcatt aactgaagaa agacgacaac
     7381 aaatatgtaa cgatgctgtt aaagttgcac gtgcagcaaa ttatagaagt gcaggtacga
     7441 ttgaattttt agtaactgat accgcacatt actttattga aatgaatgct cgaatacaag
     7501 ttgaacatac tgttacagag atgcgagcag aacgtgattt ggttgcagca caactgtatt
     7561 tattggaaca taatcattta cctttttcac aaagcgatat tcaatttaat ggtcatgtga
     7621 tagaagcgag aataaatgcc gaaaatcctg aaaagaaatt tcaaccaaca ccaggtaaag
     7681 tgacagcgtt acatttacct caaggcttta atgtgcgagt cgattcttta ctatatcacg
     7741 gttatcaagt ttcaccatat tatgattctt tagtagctaa agtaattgta aaatctcatg
     7801 atagagcttc agcaattgat aagttgaaag tcaccttaga tgaaatggtg atagatggat
     7861 tttctacaac tgccgatttt ttatatgcag ttttaaatta tccactatat cgtgatggag
     7921 atgcaaaaga tgttgatatt aaatttttag aaaaacatca aattgttaaa ggggtggaat
     7981 tataatgcga gttgatttga attgtgattt aggcgaagca tttggaaatt attcctttgg
     8041 tggtgatcat caaattattc cgttaattac aagtgcgaat gttgcttgtg gttttcacgc
     8101 tggtgatgaa aatgtaatga atgaaacggt aaaacttgcc aaagcacata atgttgcagt
     8161 aggtgcacat cctggtttac ctgatttgaa aggctttggc agacgaaata tagatatctc
     8221 taacgacgag atttataatt tgatgattta tcaattaggt gcattacaag ggttttgtcg
     8281 cattcatcaa gttaaaatta atcatgttaa accgcatggt gcattgtatc agatgggtgc
     8341 aaaagacaga gaaatagcaa acgttatagc acaagctgtt tatgactttg atccatcact
     8401 agtgttagta ggattagcaa attcatatct aatttcagaa gcaaagaatg tcggattaat
     8461 tacagcttct gaagtgtttg ctgatagacg atacgaagat gatgggcagc tcgttagtag
     8521 aaaagaaagt gatgctgtga ttactgatac tgacgaagca cttaagcagg ttttaaagat
     8581 ggtgaaggaa aataaagtta tttcaaaaaa caataaggaa gtaacgttac aagcagatac
     8641 aatttgtgtg catggtgatg gagaacatgc attattattt gtttcgaaaa ttagagaaat
     8701 tttaatgaaa gaaggcattg atattcaatc cttatagggg gctaaaaaaa tggggagaaa
     8761 tttaaagctt aaaaaagaaa gtgactttga attcacaaaa aatcataaaa ggttattatt
     8821 aggttctgta tttttgatgg caacttctgc aattgggcca gcatttttaa cgcaaacagc
     8881 agtatttaca tcacaatttt ttgcaagttt cgcatttgcc atattactgt ctatcatcat
     8941 tgacattggt gcacaaatta atatatggcg catattagtt gtaactggtt taagaggtca
     9001 agaaatatca aataaagttg ttcctgggct tggtactgtt atctcaatat tgattgcatt
     9061 tggtggtcta gcttttaata ttggtaatat tgctggtgcc ggtttaggtt taaatgcaat
     9121 ttttggatta gatgtaaaat ggggcgcagc tattactgca atctttgcaa tattaatctt
     9181 tgtaagtaaa agtggccaaa aaattatgga cgttgtttca atgattcttg gtattgtgat
     9241 gattttagtt gtggcatatg tgatgtttgt ttctaatcca ccttatggtg atgcttttgt
     9301 gcatacattt gcgccagaac atccaatgaa attagtcttg cccatcatta cgttagttgg
     9361 tggaactgta ggtggttata ttacctttgc aggtgcacat cgtatattag actctggcat
     9421 taaaggtaag caatatttac catttgtaaa tcaatcagca attgctggta ttttaactac
     9481 aggtattatg agaacgttac tattcctagc ggtattagga gttgttgtaa caggtgtgac
     9541 actaagttct gaaaatccac cagcgtcagt ttttgaacac gcaattggac caattggaaa
     9601 gaatattttt ggtattgtgt tatttgctgc agctatgtca tcagtaattg gctcagcata
     9661 cacaagcgca acatttttaa aaacacttca taaatcactt aacgaaagaa gtaatttaat
     9721 tgtgattgtg tttatcgtta tttcaacaat gattttctta tttattggaa aaccaatcag
     9781 ccttttaatt atagcaggcg cgataaatgg atggatttta cctattacgt taggtgcaat
     9841 tttgattgca agtaaaaaga aatcaattgt tggtgattat aaacacccaa attggatgtt
     9901 catctttggt atcgttgcag tacttgttac aatattgaca ggtatattct cattcaaaga
     9961 agtacttcag ttattttaaa agacatttcg actaatagca tcactgtaaa tgttgttagt
    10021 attaggcaag atatattttg atttccggtg attcatttat aaagcataat tagatatatt
    10081 ttaagggaga gtggtgcatg acaaatgtac tgctcttttt gttgtgatga aaaatctttt
    10141 tctattgatt gagaaaatag ttatttatat agtttttaaa gaaaaaaagt gataatattt
    10201 agctaatcta atgaaaattg atttattgga caaaaaatac attttaaatg agtagaggag
    10261 gctgcaaatg ggttacagaa ctttaaaaag tatttttcat gaacacaatg aaagtaaaat
    10321 gaaggaagag tatattaaaa gatttaattc tttagcttct ttcaatacta atattaatat
    10381 catacctatg gaaaatggaa aaaaagttaa tgatttggaa tatcctctat tctttatggt
    10441 gactaaaaat ctatcaaaaa aacaagaatt aatatcaatt aatagtagaa aaattgatag
    10501 agcacttaat tctttaccat atgcagctag agaacaatat tttaatgatt tattaatcga
    10561 tgaattacaa agtactaatg aaattgaaaa tgtatttagt actaaacaag agattgcaca
    10621 tgcgttaaat aaccaagcat cagaatttct taagttcaga ggcctcgtgg atcaatataa
    10681 agagatagaa cttaataaaa aaattaaagt tgataatgta agagacatta gagcgattta
    10741 tgataaatta gtttcaaatg aaattaacga acaagataag ttagatggag agctatttcg
    10801 taaaaatttt gtcggtgtgc atgatgggtc aacgaataaa tatatacatg ttgggttaca
    10861 acctgaaacc aaaattgttg aatttatagg tgaaatgcta acatttttaa aatattttga
    10921 tgcgcctcag ccgttcaaaa tcatggctag tcattatctg tttgaatata tacatccttt
    10981 ttatgatggc aatggtagag ttggaagatt tataattgcc aagctcttga gtgattatta
    11041 tgataattat actgctttga cattttcata tgttataaat agaaataaat cgaaatatta
    11101 caaggcgttt atgactgctt caaatcattt gaattgtggt gatttgacgg aatttattga
    11161 cacaatgctt gagctattga tagcaggaca agaaagaata cttgatgagt taataccaaa
    11221 aatggatgcg actgaaaaat taacgcttta tttaactagt cattataaac agatagatta
    11281 tgaatttttg tatctccttt caatggataa attgtttgga aataaaagaa atagattaac
    11341 attaattgat ttagaaaaca tattgggagt agg
//
LOCUS       DAKTIO010000087        11256 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.087, whole genome shotgun sequence.
ACCESSION   DAKTIO010000087 DAKTIO010000000
VERSION     DAKTIO010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11256
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.087"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..1077)
                     /locus_tag="O0293_001674"
     CDS             complement(<1..1077)
                     /locus_tag="O0293_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3578 domain-containing protein"
                     /protein_id="HCY0280560.1"
                     /translation="MIDNFMQVLKLIKEKRTNNVVKKSDWDKGDLYKTLVHDKLPKQL
                     KVHIKEDKYSVVGKVATGNYSKVPWISIYDENITKETKDGYYLVYLFHPEGEGIYLSL
                     NQGWSKISDMFPRDKNAAKQRALTLSSELNKYITSNEFNTGRFYYAENKDSSYDLKND
                     YPSGYSHGSIRFKYYDLNEGFTEEDMLEDLKKFLELFNELASKVTKTSYDSLVNSIDE
                     IQEDSEIEEIRTAQKDKTLKEVEAPKGIIPKYKKGVSKTTKNDSEIEKSNKENKLTGK
                     VGEKLALNYFNELIDNKIDEDKKEQFRNILNDNPGSQHGHGYDLVAFDPTNTDKAVEK
                     FIEIKTSTSSSIEEPFFMSLNEMFA"
     gene            complement(1399..1878)
                     /gene="rlmH"
                     /locus_tag="O0293_001675"
     CDS             complement(1399..1878)
                     /gene="rlmH"
                     /locus_tag="O0293_001675"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020363758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCY0280561.1"
                     /translation="MKITILAVGKLKEKYWKQAIAEYEKRLGPYTKIDIIEVPDEKAP
                     ENMSDKEIEQVKEKEGQRILAKIKPQSTVITLEIQGKMLSSEGLAQELNQRMTQEQSD
                     FVFVIGGSNGLHKDVLQRSNYALSFSKMTFPHQMMRVVLIEQVYRAFKIMRGEAYHK"
     gene            complement(2246..4564)
                     /gene="adsA"
                     /locus_tag="O0293_001676"
     CDS             complement(2246..4564)
                     /gene="adsA"
                     /locus_tag="O0293_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored adenosine synthase AdsA"
                     /protein_id="HCY0280562.1"
                     /translation="MKALLLKTSVWLVLLFSVMGLWQVSNAAEQHTPMKAHAVTTIDK
                     ATTDKQQVPPTKEAAHHSGKEAATNVSASAQGTADDTNSKVTSNAPSNKPSTVVSTKV
                     NETRDVDTQQASTQKPTHTATFKLSNAKTASLSPRMFAANAPQTTTHKILHTNDIHGR
                     LAEEKGRVIGMAKLKTVKEQEKPDLMLDAGDAFQGLPLSNQSKGEEMAKAMNAVGYDA
                     MAVGNHEFDFGYDQLKKLEGMLDFPMLSTNVYKDGKRAFKPSTIVTKNGIRYGIIGVT
                     TPETKTKTRPEGIKGVEFRDPLQSVTAEMMRIYKDVDTFVVISHLGIDPSTQETWRGD
                     YLVKQLSQNPQLKKRITVIDGHSHTVLQNGQIYNNDALAQTGTALANIGKITFNYRNG
                     EVSNIKPSLINVKDVENVTPNKALAEQINQADQTFRAQTAEVIIPNNTIDFKGERDDV
                     RTRETNLGNAIADAMEAYGVKNFSKKTDFAVTNGGGIRASIAKGKVTRYDLISVLPFG
                     NTIAQIDVKGSDVWTAFEHSLGAPTTQKDGKTVLTANGGLLHISDSIRVYYDINKPSG
                     KRINAIQILNKETGKFENIDLKRVYHVTMNDFTASGGDGYSMFGGPREEGISLDQVLA
                     SYLKTANLAKYDTTEPQRMLLGKPAVSEQPAKGQQGSKGSKSGKDTQPIGDDKVMDPA
                     KKPAPGKVVLLLAHRGTVSSGTEGSGRTIEGATVSSKSGKQLARMSVPKGSAHEKQLP
                     KTGTNQSSSPEAMFVLLAGIGLIATVRRRKAS"
     gene            complement(4792..5592)
                     /locus_tag="O0293_001677"
     CDS             complement(4792..5592)
                     /locus_tag="O0293_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCY0280563.1"
                     /translation="MSRLIRMSVLASGSTGNATFVENEKGSLLVDVGLTGKKMEELFS
                     QIDRNIQDLNGILVTHEHIDHIKGLGVLARKYQLPIYANEKTWQAIEKKDSRIPMDQK
                     FIFNPYETKSIAGFDVESFNVSHDAIDPQFYIFHNNYKKFTILTDTGYVSDRMKGMIR
                     GSDAFIFESNHDVDMLRMCRYPWKTKQRILGDMGHVSNEDAAHAMTDVITGNTKRIYL
                     SHLSQDNNMKDLARMSVGQVLNEHDIDTEKEVLLCDTDKAIPTPIYTI"
     gene            complement(5982..6770)
                     /locus_tag="O0293_001678"
     CDS             complement(5982..6770)
                     /locus_tag="O0293_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HCY0280564.1"
                     /translation="MNWKLTKTLFIFVFILVNIVLVSIYVNKVNRSHINEVESNNEVN
                     FQQEEIKVPTSILNKSVKGIKLEQITGRSKDFSSKAKGDSDLTTSDGGKLLNANISQS
                     VKVSDNNLKDLKDYVNKRVFKGAEYQLSEISSDSVKYEQTYDDFPILNNSKAMLNFNI
                     EDNKATSYKQSMMDDIKPTDGADKKHQVIGVRKAIEALYYNRYLKKGDEVINARLGYY
                     SVVNETNVQLLQPNWEIKVKHDGKDKTNTYYVEATNNNPKIINH"
     gene            complement(6771..8105)
                     /locus_tag="O0293_001679"
     CDS             complement(6771..8105)
                     /locus_tag="O0293_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280565.1"
                     /translation="MNNKEHIKSVILALLVLMSVVLTYMVWNFSPDIANVDNTDSKKS
                     ETKPLTTPMTAKMDTTITPFQIIHSKNDHPEGTIATVSNVNKLTKPLKNKEVKSVEHV
                     RRDHNLMIPDLNSDFILFDFTYDLPLSTYLGQVLNMNAKVPNHFNFNRLVIDHDADDN
                     IVLYAISKDRHDYVKLTTTTKNDHFLDALAAVKKDMQPYTDIITNKDTIDRTTHVFAP
                     SKPEKLKTYRMVFNTISVEKMNAILFDDSTIVRSSKSGVTTYNNNTGVANYNDKNEKY
                     HYKNLSEDEASSSKMEETIPGTFDFINGHGGFLNEDFRLFSTNNQSGELTYQRFLNGY
                     PTFNKEGSNQIQVTWGEKGVFDYRRSLLRTDVVLNSEDNKSLPKLESVRSSLANNSDI
                     NFEKVTNIAIGYEMQDNSDHNHIEVQINSELVPRWYVEYDGEWYVYNDGRLE"
     gene            complement(8098..9924)
                     /gene="walK"
                     /locus_tag="O0293_001680"
     CDS             complement(8098..9924)
                     /gene="walK"
                     /locus_tag="O0293_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HCY0280566.1"
                     /translation="MKWLKQLQSLHTKLVIVYVLLIIIGMQIIGLYFTNNLEKELLDN
                     FKKNITQYAKQLEISIEKVYDEKGSVNAQKDIQNLLSEYANRQEIGEIRFIDKDQIII
                     ATTKQSNRSLINQKANDSSVQKALSLGQSNDHLILKDYGGGKDRVWVYNIPVKVDKKV
                     IGNIYIESKINDVYNQLNNINQIFIVGTAISLLITVILGFFIARTITKPITDMRNQTV
                     EMSRGNYTQRVKIYGNDEIGELALAFNNLSKRVQEAQANTESEKRRLDSVITHMSDGI
                     IATDRRGRIRIVNDMALKMLGMAKEDIIGYYMLSVLSLEDEFKLEEIQENNDSFLLDL
                     NEEEGLIARVNFSTIVQETGFVTGYIAVLHDVTEQQQVERERREFVANVSHELRTPLT
                     SMNSYIEALEEGAWKDEELAPQFLSVTREETERMIRLVNDLLQLSKMDNESDQINKEI
                     IDFNMFINKIINRHEMSAKDTTFIRDIPKKTIFTEFDPDKMTQVFDNVITNAMKYSRG
                     DKRVEFHVKQNPLYNRMTIRIKDNGIGIPINKVDKIFDRFYRVDKARTRKMGGTGLGL
                     AISKEIVEAHNGRIWANSVEGQGTSIFITLPCEVIEDGDWDE"
     gene            complement(9937..10638)
                     /locus_tag="O0293_001681"
     CDS             complement(9937..10638)
                     /locus_tag="O0293_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0280567.1"
                     /translation="MARKVVVVDDEKPIADILEFNLKKEGYDVYCAYDGNDAVDLIYE
                     EEPDIVLLDIMLPGRDGMEVCREVRKKYEMPIIMLTAKDSEIDKVLGLELGADDYVTK
                     PFSTRELIARVKANLRRHYSQPAQDTGNVTNEITIKDIVIYPDAYSIKKRGEDIELTH
                     REFELFHYLSKHMGQVMTREHLLQTVWGYDYFGDVRTVDVTIRRLREKIEDDPSHPEY
                     IVTRRGVGYFLQQHE"
BASE COUNT         3174 a         2198 c         1680 g         4204 t
ORIGIN      
        1 agcaaacatt tcatttagcg acataaaaaa tggttcctca atactagaag atgtagatgt
       61 tttaatttca ataaattttt ctacagcttt atctgtattt gttggatcaa aagctactaa
      121 atcatagcca tgaccgtgtt gagagcctgg attatcattt aaaatattcc taaactgttc
      181 tttcttatct tcgtctattt tattatcaat tagctcatta aagtaattta gcgctaattt
      241 ttctccaact ttaccggtta atttattctc tttatttgat ttttcaattt ctgaatcatt
      301 tttagtagtc tttgatacac cttttttata ttttggaatt attcctttag gtgcttccac
      361 ttccttgagt gtcttatctt tttgtgctgt tctaatttct tcaatttcgc tgtcttcctg
      421 tatttcgtct atgctattga ccaagctatc ataggatgtt tttgtaactt ttgaagctaa
      481 ttcattaaat agttctaaaa atttctttaa atcctctagc atatcttctt ctgtgaatcc
      541 ttcattcaaa tcataatatt tgaatcttat tgatccatga gaatatcctg atggataatc
      601 attttttaaa tcataagatg aatctttatt ttctgcgtaa taaaatcttc cagtattaaa
      661 ttcatttgat gtaatatatt tattgagttc ggaagataaa gttaatgctc tttgttttgc
      721 agcattttta tcccgcggaa acatatcact tatctttgac catccttgat tcaaagataa
      781 gtatatgcct tctccttccg gatgaaaaag atataccaaa taatatccat cctttgtttc
      841 ttttgttata ttctcatcat atattgaaat ccaaggaact ttactatagt tcccagtagc
      901 aaccttccct acaactgaat atttatcttc ttttatatgc acttttaact gcttgggtaa
      961 cttatcatgg actaaagttt tatatagatc acctttatcc caatcagatt ttttaactac
     1021 attattggta cgtttctctt taattaattt aaggacctgc ataaagttgt ctatcatttg
     1081 aaattccctc ctattataaa atatattatg tctcattttc ttcaatatgt acttatttat
     1141 attttaccgt aatttactat atttagttgc agaaagaatt ttctcaaagc tagaactttg
     1201 cttcactata agtattcagt ataaagaata tttcgctatt atttacttga aatgaaagac
     1261 tgcggaggct aactatgtca aaaatcatga acctcattac ttatgataag cttcttaaaa
     1321 acataacagc aattcacata aacctcatat gttctgatac attcaaaatc cctttatgaa
     1381 gcggctgaaa aaaccgcatc atttatgata tgcttctcct cgcataatct taaatgctct
     1441 gtacacttgt tcaattaaca caacccgcat catttgatgt gggaatgtca ttttgctgaa
     1501 tgatagtgcg tagttactgc gttgtaagac gtccttgtgc aggccgtttg atccgccaat
     1561 gacgaaaaca aagtcgcttt gctcttgggt catgcgttgg ttcaattctt gggccaatcc
     1621 ttcggaagat agcatctttc cttgtatttc taatgtaatg actgtggatt gtggtttgat
     1681 tttggctagt attcgttggc cttctttttc ttttacttgc tcaatttctt tgtcactcat
     1741 attttctggt gctttttcgt ctggaacttc tatgatgtct atcttggtgt atgggcctaa
     1801 acgtttttca tattctgcta tggcttgctt ccaatatttc tcttttagtt tccctacagc
     1861 taaaatggtg attttcatgt cgtttggtcc tccaaattgt tatcaacttt ccagttatcc
     1921 acaagttatt aacttgttca cactgttccc tcttattata ccaatatttt ttgcagtttt
     1981 tgatattttc ctgacattta tgcccgattt ttagtgtata acctgtgtat ttgtggataa
     2041 ctctgtgggc attgtgggta tttctcttta aattgtacac aattctggat aactatcccc
     2101 atttctgtgg ataaacacct tgatgtctta ttacttatcc tcgtttttat atcccatatt
     2161 tatcaacggt tatcgctttt tttcataaaa aaacactacc gtacctctta tttaagaaat
     2221 acagtagtat tattttcaat atattttagc tagcttttct acgtcgtaca gtcgcgatta
     2281 aacctatacc tgctaataat acaaacatcg cttctgggct tgaactttga ttagttccag
     2341 tttttggtaa ctgtttctca tgcgcgctac ctttaggcac tgacattcta gccaattgtt
     2401 tcccactctt gcttgataca gtagctcctt ctattgtgcg accagaacct tctgtaccgc
     2461 tactaacagt tcctctatgc gctagcaaca atacaacttt acctggagct ggttttttcg
     2521 ctggatccat cactttgtcg tcaccaattg gttgtgtatc tttaccagac ttactacctt
     2581 tgctaccttg ttgtccttta gctggttgtt cacttactgc tggtttacct aataacatac
     2641 gttgtggttc tgtcgtatca tacttagcta agttagctgt ttttaaataa cttgctagta
     2701 cttgatctaa tgaaatacct tcttctctag gaccaccgaa catactatat ccgtcgccac
     2761 ctgatgctgt gaagtcattc atcgttacgt gatatacacg ttttaaatca atattttcaa
     2821 acttacctgt ctctttattt aaaatttgaa tagcattaat tcgtttgcca gacggtttat
     2881 ttatatcata gtaaacacgg attgaatcag agatatgtag taaaccgcca ttcgctgtta
     2941 acactgtctt accgtccttt tgtgttgttg gtgcgcctaa actatgttcg aaagccgtcc
     3001 agacgtctga accttttaca tcaatttgcg caatcgtatt tccaaatggt aatactgaga
     3061 ttaaatcata gcgtgtcacc ttaccttttg cgatagaggc acgaatacct ccaccatttg
     3121 tcacggcaaa gtcagtcttt ttagagaaat tcttaacgcc atacgcttcc atagcatctg
     3181 caatcgcgtt tcctaaattt gtttcacgcg ttctaacgtc atctctttct cctttgaaat
     3241 caatggtatt gtttggaata attacctctg cagtttgtgc tctaaatgtt tgatcagctt
     3301 gattaatttg ttcagctaat gctttgttcg gtgttacatt ttcaacgtct ttaacattaa
     3361 tcaatgacgg tttaatattc gatacctctc cattgcgata attaaatgta atcttaccga
     3421 tattcgcaag tgctgtacct gtttgtgcca atgcatcatt gttataaatt tgaccatttt
     3481 gaagtactgt atgtgaatga ccatcaataa ctgtaatacg tttcttcaat tgtggatttt
     3541 gacttaattg tttcactaag taatcaccac gccatgtttc ttgtgttgaa ggatcaattc
     3601 ctaaatgtga tataacaaca aatgtatcta cgtctttata aatacgcatc atttccgctg
     3661 tcacactttg taatggatct ctaaattcaa cgcctttaat gccttcaggt cttgttttcg
     3721 tctttgtttc tggtgtcgtt acaccaataa ttccataacg aataccattt tttgttacaa
     3781 tcgttgaagg cttaaacgcg cgttttccat ctttataaac gttagtactt agcatcggga
     3841 agtctaacat accctctaac tttttcaact gatcgtatcc aaagtcaaat tcatggttac
     3901 cgactgccat agcatcataa cctactgcat tcattgcttt agccatttct tcacctttag
     3961 actggtttga aagtggtaaa ccttggaagg cgtctcctgc gtctaacatt aaatcaggct
     4021 tttcttgttc ttttactgtt tttaatttag ccataccgat gacacgccct ttttcttcgg
     4081 ctagtcggcc atggatatca tttgtatgta atattttatg tgttgttgtt tgtggtgcat
     4141 tagcagcaaa cattcgtggt gaaagtgatg ctgttttagc atttgataat ttgaacgttg
     4201 ctgtgtgagt tggtttttgt gttgaggctt gttgtgtatc tacgtcgcgt gtttcgttta
     4261 cttttgttga aactactgta gatggtttgt tagatggtgc gttggatgtt actttgctgt
     4321 ttgtatcatc agctgttccc tgcgctgatg ctgatacgtt ggttgccgct tctttgccag
     4381 aatgatgagc cgcttccttt gttggcggta cttgttgctt atctgttgtt gctttgtcta
     4441 tcgttgttac tgcatgtgct ttcattggtg tatgctgctc agccgcgttc gagacttgcc
     4501 ataatcccat tacactaaaa agcaaaacga gccatacact tgttttaagt aataaagctt
     4561 tcatttcgtt ccctccatta actgtcatac accaatatat tatcataatc cttatattaa
     4621 aatgattgat tttatgtaaa ttatttataa attacattta atatcgaatt attttacaca
     4681 tttcaatcaa gccgattcaa ctcaacaaca gccatttccc accaacaaca taaaaaagca
     4741 cccgataaag tcgtctcaca gcaatgcgga actttatcgg atgactctca tttatattgt
     4801 atatattggc gttggaatag ctttatccgt atcacatagc aatacttctt tttccgtatc
     4861 aatatcgtgt tcgttcaata cttggccaac actcatacgc gccaaatctt tcatgttatt
     4921 gtcttgtgat aaatgcgata ggtaaatacg tttcgtgtta cctgtaatca cgtctgtcat
     4981 tgcatgagcc gcatcctcat tagatacatg acccatatcg cctaaaatac gttgtttcgt
     5041 cttccatgga taacgacaca ttctcaacat atcgacgtca tgattactct caaaaataaa
     5101 cgcatcgctg ccacgtatca tacctttcat acgatcagac acgtaacccg tatccgttaa
     5161 aatcgtaaac ttcttatagt tattatggaa aatataaaat tgcggatcta ttgcatcatg
     5221 tgacacgtta aacgattcaa catcgaaacc tgcaatagat tttgtttcat aaggattaaa
     5281 aatgaatttc tgatccatag ggatgcgact atctttcttt tcaattgcct gccaagtctt
     5341 ttcattcgca taaattggca attgatattt acgcgccaaa acacctaatc ctttaatatg
     5401 atcaatatgt tcatgggtta ctaaaatacc atttaaatct tgaatattac ggtcaatttg
     5461 actaaacaat tcttccattt tctttccagt caaaccaaca tcaactaata gactaccttt
     5521 ttcattttct acaaaagtgg cgttacctgt actaccactt gctaatacac tcatgcgtat
     5581 caagcggctc atcctttctc tttaaacagt caatctctct atatgactgc aatatacatc
     5641 agtgcaaata tacgattttc cctacagaaa ctatcagtat attagctgtt ttgtatacct
     5701 ttcctattat gtaactttag ttcatcgacc ggtttcttat atttaaattt tgataatcac
     5761 gtgcaatgaa tttcttaatt attataacaa tgattagaca aatactaaag tttttaaaat
     5821 aacagttaca ctaattttac aatgcggttc tacttaaaat atgaataaat gcgcacgttg
     5881 gagacgcaaa aacatcagcc ataataaacc gtaccagcaa aaaagctagc accgcttctc
     5941 acatatgatg agcttgcaat gctagcttat tacgattcat attaatgatt aataatttta
     6001 gggttattat ttgtcgcttc gacatagtaa gtattcgttt tatccttacc gtcatgcttc
     6061 actttaattt cccagtttgg ttgtaacaat tgaacattcg tttcattcac gactgagtag
     6121 taaccgagtc tagcattaat gacttcatca ccttttttca agtaacgatt ataatataat
     6181 gcctcgattg cttttctcac accaatcact tgatgcttct tatctgcacc atctgtgggc
     6241 ttaatgtcat ccatcattga ttgtttataa ctagtcgctt tgttatcttc tatattaaag
     6301 ttcaacatcg ctttactgtt atttaaaatc ggaaaatcat catacgtttg ttcatatttt
     6361 acagaatctg aactaatctc gcttaattga tattcagcac ctttaaatac gcgcttgtta
     6421 acataatctt tcaaatcttt taagttattg tcactgacct ttaccgattg actaatgttc
     6481 gcattcaata attttccacc atctgatgtg gtcaaatccg aatcgccttt agctttagaa
     6541 ctaaagtctt ttgatcgccc tgtaatttgc tctaatttta tacctttaac tgatttattc
     6601 aatatactag tcggtacttt aatttcttct tgctgaaaat taacttcatt gttactctcg
     6661 acttcattaa tgtgtgagcg attgacttta ttaacataaa tcgatactaa cacgatgttg
     6721 acaagaataa acacgaaaat gaaaagtgtc tttgtcagtt tccagttcat ttattcaagc
     6781 ctcccatcgt tataaacata ccattcgcca tcatattcta cataccaacg cggtacgagt
     6841 tcactgttaa tctgcacttc aatgtgatta tgatctgaat tatcctgcat ttcgtaaccg
     6901 atagcgatgt ttgttacttt ttcaaaatta atatcactat tgttcgctaa gcttgaacgt
     6961 acagactcta atttcggcaa cgatttatta tcctcactat ttaaaacaac gtcggtgcgt
     7021 aataacgaac gacgatagtc aaagacgcct ttttcacccc aagtgacttg aatttgatta
     7081 gaaccttctt tattaaacgt tggataacca ttaaggaaac gttgatatgt taactcgcct
     7141 gactgattat tcgtactaaa caatctaaag tcttcgttta agaaaccacc atgaccatta
     7201 ataaaatcaa aggttcctgg aatcgtttct tccattttgc tggaactcgc ttcatcttcg
     7261 gacaggtttt tataatgata tttttcattt ttatcgttat agtttgcgac acctgtatta
     7321 ttgttgtagg ttgtaacacc actctttgaa ctacgaacga tggttgaatc gtcaaatagt
     7381 atagcattca ttttctcaac actaatcgtg ttaaatacca tgcgatatgt ttttaacttt
     7441 tcaggtttac ttggtgcaaa aacatgcgtc gtacgatcaa ttgtatcttt gtttgtgatg
     7501 atatctgtgt atggttgcat atcttttttc actgctgcta atgcatctaa aaaatgatca
     7561 tttttcgttg tagttgttaa ttttacgtaa tcgtggcgat ctttgcttat agcataaagc
     7621 acgatattat catcagcatc atgatctatg accaaacgat tgaaattgaa atgatttggt
     7681 actttcgcat tcatgttcag tacttgacca agatatgttg ataacggtaa atcatacgta
     7741 aaatcgaata atataaaatc actgttcaaa tcaggaatca tcaagttatg atcacgacga
     7801 acatgttcca cggacttcac ttctttattt ttcaaaggtt tcgtcagttt attcacatta
     7861 gataccgtcg caatcgttcc ttctggatga tcatttttcg aatgaataat ctgaaatggc
     7921 gtaatagttg tatccatttt ggctgtcata ggtgtcgtta aaggtttcgt ttcactcttc
     7981 ttactatctg tattgtcgac atttgcaata tcaggagaaa agttccatac catatatgtc
     8041 aatacgacac tcatcaagac gagtagtgct aaaatgacag atttaatatg ctccttatta
     8101 ttcatcccaa tcaccgtctt caatgacttc acatggaagt gtgataaaga tagatgtacc
     8161 ttgaccttct acactgtttg cccaaatacg accattgtgc gcttccacaa tctctttcga
     8221 aatggctagt cctaatccag taccacccat tttacgcgta cgtgccttat ctacacgata
     8281 gaatcggtcg aatatcttat cgactttatt gataggaata ccaatgccat tatctttaat
     8341 acgaatcgtc attcgattat aaagtggatt ttgtttcacg tggaactcga cacgtttatc
     8401 gcctctagaa tatttcatcg catttgtaat gacattatca aatacttgcg tcattttatc
     8461 aggatcaaat tctgtgaaaa tcgtcttttt cggaatatct cgaataaatg ttgtatcttt
     8521 cgcagacatt tcatgtcgat taataatttt attaatgaac atgttaaagt cgataatttc
     8581 tttgttgatt tgatcagact cattatccat tttagataac tgtagcaagt cattgaccag
     8641 tcgaatcatt cgttctgttt cttcacgggt aacagataaa aattgtggcg caagttcctc
     8701 atctttccat gcaccttctt caagtgcttc aatgtaacta ttcatagaag ttaaaggtgt
     8761 acgtaactca tgtgatacat tggcaacaaa ttcacgacgc tcacgttcaa cttgttgttg
     8821 ttcagttacg tcatgtaaca cagcgatata accagttaca aatcctgttt cctgcacaat
     8881 cgtactaaag ttaacacgtg cgattagacc ttcttcttca tttaaatcta ataagaaact
     8941 atcattattc tcttgaattt cttccagttt aaattcatct tcaagactta atacacttaa
     9001 catgtaatat ccgatgatgt cttctttcgc cataccaagc atcttgagtg ccatatcatt
     9061 gacgatacga atacgtccac ggcggtctgt tgcaataata ccatcactca tatgggtgat
     9121 aactgagtcc agtctacgtt tctcactttc agtattagcc tgcgcttctt gtacacgttt
     9181 agacaagtta ttaaatgcta aagctaattc gccaatttca tcattaccat aaatcttcac
     9241 acgttgcgta tagttacctc tggacatttc gaccgtctgg ttacgcatat cggtgattgg
     9301 tttggtaatc gttcgcgcta taaagaatcc taggatgact gtgattaata atgaaatagc
     9361 tgtaccaaca atgaatattt gatttatatt atttaattgg ttataaacgt cattaatttt
     9421 tgattcgata taaatattac caattacctt tttatcgact ttaactggga tattatatac
     9481 ccagacacgg tccttaccac cgccataatc ttttaaaatt aaatgatcgt ttgattgtcc
     9541 tagtgatagt gctttttgga cagaactatc attcgctttt tgattgatta gactacggtt
     9601 agactgcttc gtcgtcgcaa taataatttg gtctttatct ataaaacgaa tttctccaat
     9661 ttcttgacgg ttggcatact cacttaataa attttgaata tctttttgtg catttacgga
     9721 gcccttttcg tcatatactt tttcaatact aatttctaat tgtttcgcgt actgcgtaat
     9781 attcttctta aaattatcaa gcagctcttt ttcaaggtta tttgtaaaat ataacccgat
     9841 aatttgcata ccaatgataa tcagtaatac ataaacaatt acaagtttag tatgaaggga
     9901 ttgtagttgt tttagccact tcattcgttt cgacctctac tcatgttgtt ggaggaaata
     9961 tccaacgcct ctacgcgtca caatatattc aggatgtgac ggatcatctt caatcttttc
    10021 acgtaaacga cgaatcgtta catcgaccgt acgtacatcg ccaaagtaat catagcccca
    10081 tactgtttgt aataaatgtt cacgtgtcat tacttgtccc atatgttttg ataaataatg
    10141 gaacaattca aattcacgat gtgttaattc aatatcttcg ccacgttttt taatagaata
    10201 tgcgtctgga taaatcacaa tatctttaat tgtgatttca ttcgttacat ttccagtgtc
    10261 ttgtgctggt tgtgagtaat gacgacgtaa gttcgctttc acacgtgcga ttaattcacg
    10321 cgtactaaac ggtttcgtta catagtcatc tgcacctagt tctaaaccaa gcactttatc
    10381 aatttctgaa tctttagcag taagcattat tattggcatt tcgtattttt tgcgcacttc
    10441 acgacatact tccataccat cacgaccagg taacatgata tctagtaata cgatgtctgg
    10501 ttcttcttca taaattaagt cgactgcatc attaccatcg tatgcacagt acacatcgta
    10561 tccttctttt tttaagttaa attctaaaat atcagcaatc ggtttttcat catcaactac
    10621 aacaactttt ctagccattt gcataaacct cttttcttaa atcattaata ttattttatc
    10681 attttttgtt ctttatcgtt tatgtttgtg taaaaaatca caggatgtcg cttactattt
    10741 ttgttattgt gcgcattttc cgtcttataa cgtatagtaa cctattatat aatttaccat
    10801 ttataagcta ttaattctat tttccaatat aaacttatat attactattt tttattagtt
    10861 tctcacggcg gattgtgaca atgatattta acacaatgat gaggtctcta ttccaataat
    10921 aaaattagga taatggcaac ttctagttgc acgtcattgc tcagaaaata aatatcatta
    10981 ttcgttattc gttactttaa caattgatgt tatggaggac tggtggcata tcacaacgaa
    11041 gaaatatgtt gacagtagca tatgtttgta cgtctaataa agattttatt gtctacgttt
    11101 atctttcata tccaaccact aagttacttg aggttcatac attttaaaga aaatggacga
    11161 acagataaat caatatgatt atcattcgtc catatatctt ttattaatat aatattaaac
    11221 gcaaaaaaat gcctatcctc ttggataggc atttgt
//
LOCUS       DAKTIO010000088        11213 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.088, whole genome shotgun sequence.
ACCESSION   DAKTIO010000088 DAKTIO010000000
VERSION     DAKTIO010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11213
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.088"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            26..787
                     /locus_tag="O0293_001682"
     CDS             26..787
                     /locus_tag="O0293_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0280568.1"
                     /translation="MSKEAGHTFLAKLGKTRLRPGGKEATDWLIQQGAFSQDKQVLEV
                     ACNMCTTSIYLAHTYGCHIQGVDINKKALEKAQENISAAGLESYIQVQQANAVKLPFD
                     DNQFDIVLNEAMLTMLPIAIKEKALREYYRVLKPGGILLTHDIVIVNESHATHVVKSL
                     SAAINVNVSPQTKLGWLDLYNQAGFNHVHYHTGPMSLMTPKGLIYDEGIVGTIKIINN
                     ALKKENRPMFCKMFKTMTKLRKDMNYITFVAKKEH"
     gene            complement(1221..2657)
                     /locus_tag="O0293_001683"
     CDS             complement(1221..2657)
                     /locus_tag="O0293_001683"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCY0280569.1"
                     /translation="MTKLPQNFMWGGALAANQFEGGYDKGGKGLSVIDVMTSGAHGKA
                     RQITESIDPNHYYPNHEGIDFYHRYKEDIALFKEMGLKCLRTSIAWTRIFPNGDEDVP
                     NEEGLAFYDRIFDELIAQGIEPVVTLSHFEMPLHLAKHYGGFRNREVVDYFVHFARVV
                     FERYKDKVTYWMTFNEINNQMDTSNPIFLWTNSGVALTENDNPEEVLYQVAHHELLAS
                     ALAVRLGKEINPKFKIGTMISHVPIYPYSCHPKDMMEAQIANRLRFFFPDVQVRGYYP
                     SYAKKMLARKGYDVGWQEGDDSILQQGTVDYIGFSYYMSTAVKHDVDTTVENNIVNGG
                     LNHSVENPHIATSDWGWAIDPDGLRYTLNVLYDRYQLPLFIVENGFGAVDEVVDGHIH
                     DDYRIEYLKAHITAAIEAVDQDGVDLIGYTPWGIIDIVSFTTGEMKKRYGLIYVDRDN
                     DGHGTMERLKKDSFYWYQQVIASNGDKL"
     gene            complement(2673..3464)
                     /locus_tag="O0293_001684"
     CDS             complement(2673..3464)
                     /locus_tag="O0293_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCY0280570.1"
                     /translation="MSNKYKEQAQDILTAVGGVENIVDATYDTKCITIHMQHTIPSTA
                     NEVKQIVDVTSVAENDTQLVIKLNGNVDEVYQQLQRLIKNANVEESENTDNINSQDTS
                     YTPQVKVTTPILVKAPIAGRRILLKEVRDSIFREKMVGEGLAIKAHEESKVIAPFNGL
                     ISMIVPTKHAVGIQSEDGVDIVIHIGLNTVDLEGKGFKCFVKQNDHVEAGQTLLQFDQ
                     QYIQQQGYNADVIVVISNSADLGKVELTMNEIITTEDVIFKIFKN"
     gene            3613..4317
                     /locus_tag="O0293_001685"
     CDS             3613..4317
                     /locus_tag="O0293_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCY0280571.1"
                     /translation="MLKYEHIAKQLNAFIHQSNFKPGDKLPSVTQLKERYQVSKSTII
                     KALGLLEQDGLIYQAQGSGIYVRNIADANRINVFKTNGFSKSLGEHRMTSKVLVFKEI
                     ATPPKSVQDELQLNADDTVYYLERLRFVDDDVLCIEYSYYHKEIVKYLNDDIAKGSIF
                     DYLESNMKLRIGFSDIFFNVDQLTSSEASLLQLSTGEPCLRYHQTFYTMTGKPFDSSD
                     IVFHYRHAQFYIPSKK"
     gene            complement(4425..5126)
                     /gene="lrgB"
                     /locus_tag="O0293_001686"
     CDS             complement(4425..5126)
                     /gene="lrgB"
                     /locus_tag="O0293_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HCY0280572.1"
                     /translation="MINHLALNTPYFGILLSVIPFFLATILFEKTNRFFLFAPLFVSM
                     VFGVAFLYLTGIPYKTYKIGGDIIYFFLEPATICFAIPLYKKREVLVKHWHRIIGGIG
                     IGTVVALLIILTFAKLAQFANDVILSMLPQAATTAIALPVSAGIGGIKELTSLAVILN
                     GVIIYALGNKFLKLFRITNPIARGLALGTSGHTLGVAPAKELGPVEESMASIALVLVG
                     VVVVAVVPVFVAIFF"
     gene            complement(5119..5562)
                     /gene="lrgA"
                     /locus_tag="O0293_001687"
     CDS             complement(5119..5562)
                     /gene="lrgA"
                     /locus_tag="O0293_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like murein hydrolase modulator LrgA"
                     /protein_id="HCY0280573.1"
                     /translation="MVVKQQKDASKPAHFFHQVIVIALVLFVSKIIESFMPIPMPASV
                     IGLVLLFVLLCTGAVKLGEVEKVGTTLTNNIGLLFVPAGISVVNSLGVISQAPFLIIG
                     LIIVSTILLLICTGYVTQIIMKVTSRSKGDKVTKKIKIEEAQAHD"
     gene            complement(5675..6415)
                     /locus_tag="O0293_001688"
     CDS             complement(5675..6415)
                     /locus_tag="O0293_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LytR"
                     /protein_id="HCY0280574.1"
                     /translation="MKALIIDDEPLARNELTYLLNEIGGFEEINEAENVKETLEALLI
                     NQYDIIFLDVNLMDENGIELGAKIQKMKEPPAIIFATAHDQYAVQAFELNATDYILKP
                     FGQKRIEQAVNKVRATKAKDDNNASAIANDMSANFDQSLPVEIDDKIHMLKQQNIIGI
                     GTHNGITTIHTTNHKYETTEPLNRYEKRLNPTYFIRIHRSYIINTKHIKEVQQWFNYT
                     YMVILTNGVKMQVGRSFMKDFKASIGLL"
     gene            complement(6418..8172)
                     /locus_tag="O0293_001689"
     CDS             complement(6418..8172)
                     /locus_tag="O0293_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCY0280575.1"
                     /translation="MLSLTMLLLERVGLIIILAYVLMNIPYFKNLMNRRRTWKARWQL
                     CIIFSLFALMSNLTGIVIDHQHSLSGSVYFRLDDDVSLANTRVLTIGVAGLVGGPFVG
                     LFVGVISGIFRVYMGGADAQVYLISSIFIGIIAGYFGLQAQRRKRYPSIAKSAMIGIV
                     MEMIQMLSILTFSHDKAYAVDLISLIALPMIIVNSVGTAIFMSIIISTLKQEEQMKAV
                     QTHDVLQLMNQTLPYFKEGLNRESAQQIAMIIKNLMKVSAVAITSKNEILSHVGAGSD
                     HHIPTNEILTSLSKDVLKSGKLKEVHTKEEIGCSHPNCPLRAAIVIPLEMHGSIVGTL
                     KMYFTNPNDLTFVERQLAEGLANIFSSQIELGEAETQSKLLKDAEIKSLQAQVSPHFF
                     FNSINTISALVRINSEKARELLLELSYFFRANLQGSKQHTITLDKELSQVRAYLSLEQ
                     ARYPGRFNININVEDKYRDVLVPPFLIQILVENAIKHAFTNRKQGNDIDVSVIKETAT
                     HVRIIVQDNGQGISKDKMHLLGETSVESESGTGSALENLNLRLKGLFGKSAALQFEST
                     SSGTTFWCVLPYERQEEE"
     gene            complement(8419..9093)
                     /gene="scdA"
                     /locus_tag="O0293_001690"
     CDS             complement(8419..9093)
                     /gene="scdA"
                     /locus_tag="O0293_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein ScdA"
                     /protein_id="HCY0280576.1"
                     /translation="MINKNDIVADVVTDYPKAADIFRSVGIDFCCGGQVSIEAAALEK
                     KNVDLNELLQRLNDVEQTNTPGSLNPKFLNVSSLIQYIQSAYHEPLREEFKNLTPYVT
                     KLSKVHGPNHPYLVELKETYDTFKNGMLEHMQKEDDVDFPKLIKYEQGEVVDDINTVI
                     DDLVSDHIATGELLVKMSELTSSYEPPIEACGTWRLVYQRLKALEVLTHEHVHLENHV
                     LFKKVS"
     gene            complement(9233..10957)
                     /gene="tarS"
                     /locus_tag="O0293_001691"
     CDS             complement(9233..10957)
                     /gene="tarS"
                     /locus_tag="O0293_001691"
                     /EC_number="2.4.1.355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(ribitol-phosphate)
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCY0280577.1"
                     /translation="MMKFSVIVPTYNSEKYITELLNSLAKQDFPKTEFEVVVVDDCST
                     DQTLQIVEKYRNKLNLKVSQLETNSGGPGKPRNVALKQAEGEFVLFVDSDDYINKETL
                     KDAAAFIDEHHSDVLLIKMKGVNGRGVPQSMFKETAPEVTLLNSRIIYTLSPTKIYRT
                     ALLKDNDIYFPEELKSAEDQLFTMKAYLNANRISVLSDKAYYYATKREGEHMSSAYVS
                     PEDFYEVMRLIAVEILNADLEEAHKNQILAEFLNRHFSFSRTNGFSLKVKLEDQPQWI
                     NALGDFIQAVPERVDALVMSKLRPLLHYARAKDIDNYRTVEESYRQGQYYRFDIVDGK
                     LNIQFNEGEPYFKGIDIAKPKVKMTAFKFDNHKIVTELTLNEFMIGEGHYDVRLKLHS
                     RNKKHTMYVPLSVNANKQYRFNIMLEDIKAYLPKEKIWDVFLEVQIGTEVFEVRVGNQ
                     RNKYAYTAETSALIHLNNDFYRLTPYFTKDFNNISLYFTAITLTDSISLKLKGKNKII
                     LTGLDRGYVFEEGMASVVLKDDMVMGMLSQTSENEVQILLSKDIKKRDFKNIVKLNTA
                     HITYPLNK"
     gene            complement(10990..>11213)
                     /locus_tag="O0293_001692"
     CDS             complement(10990..>11213)
                     /locus_tag="O0293_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCY0280578.1"
                     /translation="YAFDLEDYITTRDFYEPYESFVPGKIVQSFDALMDALDNEDYEG
                     EKVIPFLDKHFKYQDGRSSERLVRNLFGS"
BASE COUNT         3536 a         2123 c         1680 g         3874 t
ORIGIN      
        1 ttatcaatta aaaggaggtt atgctatgtc taaagaagct ggtcatacat ttttagctaa
       61 attaggaaaa actcgtctac gccccggtgg taaagaagca acagattggt taatacaaca
      121 aggggcattt tcacaagata aacaagtgtt agaagtggca tgtaatatgt gcacaacatc
      181 tatttatcta gctcatacat atggctgtca cattcaaggc gttgatataa ataagaaagc
      241 attagaaaaa gcacaggaaa acatttcagc agcaggtctt gaatcatata ttcaagttca
      301 acaagcgaat gctgttaaat tgccctttga tgacaatcaa ttcgatatcg ttttaaatga
      361 agcaatgtta acaatgttac ccatcgccat aaaggaaaaa gcattacgcg agtactaccg
      421 agtcttaaag cctgggggta tcttgttaac acatgatatt gtcatcgtta atgaatcaca
      481 tgccacacat gttgttaaat cattatctgc agcaattaat gtcaatgtct caccgcagac
      541 gaaacttggc tggttagatt tatataatca agctggtttt aatcatgtgc attatcatac
      601 tggtccaatg agtttaatga caccaaaagg tttaatttat gacgaaggta ttgttggaac
      661 tataaagatt atcaacaatg ctttgaaaaa agaaaatcga ccaatgtttt gtaaaatgtt
      721 taaaacgatg actaaattgc gtaaagatat gaattatatt acttttgtcg ctaaaaaaga
      781 gcactaaata taatgccact aactgtactt tgtatctatg tttgactatc actttaattt
      841 ctttgtgaca ctaatcatct acttaacaat atcgttatcg ttgattagta agtcatcaat
      901 tttggttaaa gactttcata aacactcaaa cattaacact atacatagtt agtggcatta
      961 tttttttctt aaaattttaa catcacggga ttggaacaga aatgatattt tcgcaaaatt
     1021 tatttctgtc cttccccaac ttgcattacc tgtagaattt cttttcgaat ttctctgtgt
     1081 tggggccccg ccaacttgca ttacctgtag aatttctttt cgaaattctt tgtgttgggg
     1141 gcccgccatt ataacactgt aggtcctact atattaattt atgtctcggg ctctaaccct
     1201 aaaatactta taatatacct ttataattta tctccatttg atgctatcac ttgttgatac
     1261 caatagaacg aatctttttt caagcgttcc atcgtgccat gaccatcatt atctcgatca
     1321 acatatatta aaccatagcg tttcttcatt tcaccggttg taaatgaaac aatatcaatg
     1381 attccccacg gtgtataacc gattaaatct acaccatctt gatcaactgc ttctatcgct
     1441 gctgtaatat gtgcttttaa atattcaatg cgataatcat catgaatatg tccatctacc
     1501 acttcatcaa ctgcaccaaa accattttcc acaataaaaa gtggtaactg ataacgatca
     1561 tataacacat tcaatgtata tcttaagcca tctggatcaa tcgcccaacc ccaatcactc
     1621 gttgcgatat gcggattctc cacagaatga ttcaaaccac cgttgacgat gttgttttca
     1681 actgtagtat caacatcatg ttttacagcc gtagacatgt aataactaaa gccaatataa
     1741 tcaaccgtgc cctgctgtaa aatactgtcg tccccttctt gccatccaac atcatatcct
     1801 tttcgtgcca acattttttt agcatagctt ggataataac ctctcacttg gacatccggg
     1861 aaaaagaaac gtaagcgatt cgcaatttgt gcttccatca tatctttcgg atgacacgaa
     1921 tatggataaa tgggtacatg tgaaatcatt gttccaatct taaacttcgg attaatctct
     1981 ttaccaagac gaactgctaa agcactggct aaaagttcat gatgtgctac ttgatacaag
     2041 acttcttcag gattatcatt ttctgtcaat gctaccccag aattcgtcca taaaaagata
     2101 ggatttgatg tgtccatctg attattaatt tcattaaacg tcatccaata tgtaacttta
     2161 tctttatatc tttcaaatac aacacgcgca aaatgcacaa aataatcgac aacttctcta
     2221 tttctaaatc caccataatg tttcgctaaa tgaagtggca tctcaaaatg tgataacgtc
     2281 acaacaggtt caataccttg tgcaattaat tcatcaaaga tacgatcata aaaggcgagt
     2341 ccttcttcat ttggcacatc ttcatcccca ttcggaaaga tacgtgtcca cgcaatcgac
     2401 gtacgtaaac atttcaatcc catttcttta aacaaggcaa tatcttcctt ataacgatga
     2461 taaaaatcaa taccttcatg atttggataa tagtgattgg gatctataga ttctgtaatc
     2521 tgacgtgctt tgccatgtgc accactcgtc ataacatcaa ttacacttaa ccctttacca
     2581 cctttatcat atccaccttc aaattgattt gcggcaagag cgccacccca catgaaattt
     2641 tgcggtaatt ttgtcattat tacaacacac tcctagtttt taaatatttt aaaaataaca
     2701 tcttcagtcg taatgatttc attcattgtc agttctactt ttcctaaatc ggcagagttg
     2761 ctaataacga caataacgtc agcattgtag ccttgttgtt gtatatattg ctggtcgaat
     2821 tgcaacaacg tttgccctgc ttcaacatga tcattttgct ttacaaagca cttgaaccct
     2881 ttaccttcca agtcaactgt attcaagcca atatgaatga ctatgtccac accgtcttct
     2941 gattgaatac caactgcatg cttagttggt acaatcatag atattaaacc attgaacggt
     3001 gcgattactt tggattcttc atgagctttg attgctaagc cttcacctac cattttctct
     3061 ctaaaaattg aatctcttac ttctttaagt aaaatacgac gaccagcgat tggtgctttc
     3121 actaaaattg gtgttgttac ttttacttga ggtgtataac ttgtatcttg actattaata
     3181 ttgtcagtat tctcactctc ttcgacatta gcattcttaa ttaatcgctg taattgctga
     3241 tacacttcat cgacatttcc atttaatttt atgactaact gcgtatcatt ttctgctaca
     3301 gatgtcacat caactatttg tttcacttca tttgctgtag aaggaattgt atgttgcata
     3361 tgaattgtaa tgcacttcgt atcatacgtt gcatcaacaa tgttttcgac accacctaca
     3421 gctgtaagaa tgtcttgggc ttgttcttta tatttattgc tcataaatat taccccctca
     3481 acttgatagt agaatgaaaa tgtatttact tacctccaat tataattgta ccggttcaat
     3541 ttgtaaacgc cgatacaatt ataatatttt gtgctataat aattacagac aaagtgaaac
     3601 gaggacagaa tattgttaaa gtatgaacat attgctaagc aacttaatgc gtttatacat
     3661 caatctaatt tcaaacccgg tgataaattg ccaagcgtga cgcaattaaa agaacgttat
     3721 caagtaagta agagtactat cattaaagca ttaggcttat tggaacaaga tggtttgatc
     3781 tatcaagcac aaggcagtgg tatttatgtg agaaatattg ctgatgccaa tcgtatcaac
     3841 gtctttaaga ctaatggttt ctctaaaagt ttaggtgaac accgaatgac aagtaaggta
     3901 cttgttttta aggagattgc aacgccacct aaatctgtac aagatgagct ccaattaaat
     3961 gcagatgata ccgtctacta tttagagcga ttaagattcg tggacgatga tgttttatgt
     4021 atcgaatatt cttattatca taaagaaatc gtgaaatatt taaatgatga tattgctaag
     4081 ggctctatct tcgactattt agaatcaaac atgaaacttc gtattggttt ttcagatatt
     4141 ttctttaatg tagatcaact cacttcaagt gaagcttcat tactacaatt gtctacaggt
     4201 gaaccatgtt tacgttacca ccagactttt tatacaatga ctggcaaacc ctttgattca
     4261 tctgacatcg tatttcatta tcgtcatgca cagttttata ttcctagtaa aaagtaataa
     4321 atacataaaa acgtctatat cccagttata aactggagta tagacgtttt tttacgataa
     4381 taacaatggc tcaaattgct attatcttgc ttaggttttt cgttttagaa gaatattgct
     4441 acaaagacag gcacaactgc tacaacaact acaccaacta acactaaagc tatacttgcc
     4501 attgattctt ctacaggtcc taattctttg gctggtgcta cacctaatgt gtgaccactt
     4561 gttccaagtg ctaatcctcg ggcaataggg ttagtaattc ggaaaagctt taagaattta
     4621 ttacctaggg cataaataat gacaccattt aaaataactg ctaatgatgt taattctttt
     4681 ataccaccga taccagctga tactggtaac gcaatcgctg tagttgctgc ttgaggtaac
     4741 attgataaaa taacatcatt ggcaaattgt gctaacttcg caaaagttaa aataattaat
     4801 aacgctacaa ctgtaccgat accaatacct ccgatgatac gatgccaatg tttaacaagc
     4861 acttcacgct ttttatataa cggaatcgca aaacagattg ttgccggttc taagaagaag
     4921 taaataatgt ctccacctat tttgtaagtc ttatacggaa tgcctgttaa atagaggaag
     4981 gccacaccaa ataccatact gacaaatagc ggtgcgaata agaagaaacg attagttttt
     5041 tcaaataata tggtcgctaa gaaaaatggt ataacggata acagtattcc gaagtaaggt
     5101 gtgtttagtg ctaagtggtt aatcatgagc ttgtgcctcc tctattttga tcttttttgt
     5161 gactttgtca cctttagatc tcgaagtaac tttcataata atttgtgtga catagccagt
     5221 acaaataagt aatagtattg ttgagacgat tattagtcca atgattaaaa atggtgcttg
     5281 gctaatgaca cctaaagagt taacaactga gataccggct ggtacgaaga gtaagccaat
     5341 gttatttgtt agtgtcgttc ctactttttc gacttcgcct aacttaacag caccagtaca
     5401 taataataca aataataata ctaaaccgat tactgatgca ggcataggaa ttggcataaa
     5461 tgattcaatt attttcgata caaagagtac taaagcaatt acaatgactt ggtgaaaaaa
     5521 gtgtgctggt tttgatgcgt ctttttgttg tttcacgacc attgcctcct acgtttgatt
     5581 taactaaagt atagatggct cacttcgatt tgcgtgattt ttagtccgaa atacaaaata
     5641 tcataggtaa aatgcataaa aaaaaggatt actgttaaag taatcctatc gacgctttaa
     5701 aatctttcat aaatgaacgt ccaacttgca tcttgacacc atttgtcaat attaccatat
     5761 aagtatagtt aaaccattgt tgcacttctt taatgtgttt cgtgttaata atatatgaac
     5821 gatgaatacg tataaaataa gtgggattca atcgtttttc ataacgattc aatggctctg
     5881 ttgtttcgta tttatgattc gttgtatgta tggttgtaat accattatgt gtgccaatcc
     5941 caataatatt ttgttgcttt aacatgtgaa ttttatcgtc aatttcaaca ggtaagcttt
     6001 gatcaaaatt cgccgacata tcattcgcaa ttgcacttgc gttattatca tctttggctt
     6061 tagtcgcacg cactttattg actgcttgtt caatacgttt ttgaccaaac ggtttcaaaa
     6121 tatagtctgt cgcatttaat tcaaatgcct gtactgcgta ttggtcatgt gcagttgcaa
     6181 aaataatcgc aggtggctct ttcatctttt gaatcttagc tcctaattcg atcccatttt
     6241 catccattaa attgacatct aaaaatataa tgtcatattg attgatcagt agtgcttcca
     6301 atgtttcttt tacattttct gcctcattaa tttcttcaaa accaccaatt tcatttaata
     6361 aatatgttaa ttcattacgt gctaatggct catcatctat gattaatgct ttcatattta
     6421 ttcctcctct tgtctttcat aaggaagtac acaccaaaaa gtggtaccgc tcgatgtcga
     6481 ttcaaattgt aatgctgcgg attttccaaa taatcctttt aggcgtaagt ttaaattttc
     6541 taaagcacta ccagttccag actctgattc tacagatgtt tctcccaaca aatgcatttt
     6601 atctttagaa ataccctgac cattatcttg tacaataata cgtacatgtg ttgcagtttc
     6661 tttaatcact gacacgtcaa tatcgttacc ttgctttcga ttcgtaaacg catgtttgat
     6721 ggcattttca actaaaattt gaattaaaaa tggtggtaca agcacatcgc gatatttgtc
     6781 ttcaacatta atattgatat taaatcttcc tggataacgt gcttgttcga gtgataagta
     6841 tgcacgcact tgacttaact ctttatctaa agtaatcgta tgttgcttag agccttgtaa
     6901 attcgctctg aaaaaataac tcaattctaa tagtaactct cgtgcctttt cgctatttat
     6961 tctaactaaa gctgagatcg tgttaattga attgaagaaa aaatgtggac tcacttgtgc
     7021 ctgtaatgac ttaatctcag catctttcaa taacttactt tgcgtttcgg cttcaccaag
     7081 ttcaatttgg ctactaaaaa tatttgccaa tccttctgca agttgacgtt ccacaaaagt
     7141 taaatcatta gggtttgtaa aatacatctt caatgtaccg acgatagaac catgcatctc
     7201 aagtggtatc acgatagctg ctctaagcgg gcaattcgga tgactacaac caatctcttc
     7261 tttagtatga acttctttca actttcctga tttcaataca tctttagaca gacttgttaa
     7321 tatttcattt gttggtatgt gatgatcact acctgcacct acatgcgata agatttcatt
     7381 tttgcttgta attgctacgg cagatacttt cattaaattt ttaataatca tcgcaatttg
     7441 ctgtgccgat tctctattca atccttcttt aaaatacggc aatgtctggt tcatcaattg
     7501 cagtacatca tgtgtttgaa cagccttcat ttgctcctct tgctttaatg ttgaaatgat
     7561 aatagacata aaaatcgccg taccaacgct attaacaata atcattggta gtgcaattaa
     7621 tgatatgagg tcaaccgcat atgctttgtc gtgggaaaat gttaaaatgc tcaacatttg
     7681 aatcatttcc ataacaattc caatcatggc acttttcgca atactcgggt aacgcttgcg
     7741 tctttgagct tgtaagccaa aataaccagc aattatacca ataaatatag atgagataag
     7801 ataaacttgt gcatccgccc cacccatata cactctgaaa atacctgaaa taacgccaac
     7861 aaatagacct acaaaagggc caccaactaa tcctgcgaca cctatcgtta atacacgtgt
     7921 gttagctaaa gatacatcat catctaaacg gaagtacaca cttcctgaca aactatgttg
     7981 atgatcgatg acgataccag ttaaattaga cattaaggca aacaaactga aaataataca
     8041 taattgccaa cgtgctttcc atgtacgtcg acgattcatt aagtttttaa aatatggaat
     8101 attcatcaac acataggcca aaataataat taaacctaca cgctcaagta ataacattgt
     8161 tagcgatagc acgatagcac ctcagtgaat tataattatg tttattttat cataatagtg
     8221 catgtaaaat tcatgaataa attattaaaa aaacacgcta ctatttaatt atctatacca
     8281 ttaaattttc atatcaattt ttatgctaag ttgaccattt ttcttttcaa ataatgctgt
     8341 ttatgtcact attatgactt ttatacatag cgaaaaactg ctggacttat ttttgccaac
     8401 agtttctaat cgcgttattt atgatacttt tttaaataat acgtgattct ctaaatgtac
     8461 gtgttcatgt gttaacactt caagtgcttt taatctctga taaacaagtc gccaagtacc
     8521 acacgcttct atcggaggtt cataactaga tgttaattcg ctcattttta ctaacaattc
     8581 tcccgttgca atgtggtctg aaactaaatc atctatcaca gtattaatat cgtctactac
     8641 ctcaccttgc tcatatttaa tgagttttgg aaaatcgaca tcgtcttctt tttgcatatg
     8701 ctctaacatg ccatttttaa atgtatcgta tgtttctttt aactcaacta aatatggatg
     8761 attaggtcca tgtactttcg ataacttcgt cacataaggt gttaaatttt taaattcttc
     8821 tcttagaggt tcatgatatg ctgattgaat atattgaata agtgatgaaa catttaaaaa
     8881 tttaggattt aacgaacctg gtgtattcgt ttgttcaacg tcgttgagac gctgtaataa
     8941 ttcgttcaaa tctacatttt tcttttctaa ggctgctgct tctatactta cttgtccgcc
     9001 acaacaaaaa tctattccca cacttctaaa aatatccgct gctttcggat aatcagttac
     9061 tacatctgct actatgtcat ttttatttat cattatagat aacctcctca tttccagtgt
     9121 atacaattta atgtatatga aaatgaaaat cagggaaatg tcataatcat gtcataaaca
     9181 gcaaaataaa gccctcaaat catgttggct cacaatgatt tgagggcatt tattatttat
     9241 ttagtggata agtgatatgt gcagtgttta acttaacaat atttttgaag tctcgctttt
     9301 taatatcttt gctaagtaag atttgcactt cgttttctga tgtttggctt aacattccca
     9361 tcaccatgtc gtcttttagt acgacactag ccataccttc ttcaaataca taaccacgat
     9421 ccagaccagt taaaatgatt ttgtttttac cttttaactt caatgagatt gaatccgtta
     9481 atgtaatagc tgtaaagtat aacgaaatgt tattaaagtc ttttgtgaaa tacggtgtta
     9541 atctataaaa atcattattc aaatgaatta atgcacttgt ttctgcagta tatgcatatt
     9601 tattacgttg attaccaaca cgcacttcaa atacttccgt acctatttgg acttctaaga
     9661 aaacatccca aattttttct ttaggtaaat acgctttaat atcttctaac ataatgttaa
     9721 aacgatattg tttattcgca ttgacactta aaggtacata cattgtgtgc ttcttgtttc
     9781 gtgaatgtaa tttaagtctg acatcataat gtccttcgcc aatcataaat tcatttaacg
     9841 ttagctctgt aacaatttta tgattatcaa atttaaatgc tgtcattttc acttttggct
     9901 tagcgatatc aatgccttta aagtatggtt cgccttcatt gaattgaatg tttaatttac
     9961 catctacaat atcaaaacgg tagtattgac cttgacggta actttcctcc acagttctat
    10021 agttgtctat atctttcgct ctcgcgtagt gcaacaatgg tcgtaattta ctcatcacca
    10081 atgcatctac acgttctgga actgcttgta taaagtctcc tagagcatta atccattgtg
    10141 gttgatcttc tagtttaact ttaagtgaga agccattcgt acgagaaaaa ctaaaatgac
    10201 gatttaaaaa ttctgctaag atttgatttt tatgggcttc ttctaaatct gcatttaata
    10261 tttctacagc aatcaatctc atgacttcat aaaagtcttc aggtgaaaca tacgcactac
    10321 tcatatgttc accttcacgc tttgtagcat aataatacgc tttatcactt aacacactga
    10381 ttcgatttgc atttaaatat gctttcattg taaataattg atcttctgca ctctttaatt
    10441 cttctggaaa ataaatgtca ttatctttta gtaatgctgt tctatagatt ttagtcgggc
    10501 ttaaagtata gataattctt gaatttaaca aagtaacttc aggtgctgtt tctttaaaca
    10561 tagattgtgg tacaccacga ccattaacac ctttcatttt aatcaataag acatctgagt
    10621 gatgttcatc aataaatgct gctgcatcct ttaaagtctc tttgtttata tagtcatcgg
    10681 agtccacaaa taatacaaat tcaccttctg cttgttttaa cgccacattt ctaggtttac
    10741 ctggaccacc agaatttgtt tcgagttgac ttactttcaa gttcaattta ttgcgatact
    10801 tttcaactat ttgtaacgtt tgatctgttg aacagtcatc aactacaacc acttcaaatt
    10861 cagttttcgg aaaatcttgt ttcgcaaggc tattaagtaa ttctgttata tacttttctg
    10921 aattgtatgt tggaactatt actgaaaatt tcatcattat acctctccca ctttgactac
    10981 tatataaact tagctaccaa ataaatttct gactaaacgc tcacttgatc ggccatcttg
    11041 atatttaaaa tgtttatcta agaatggaat gactttttct ccttcataat cttcattgtc
    11101 caaggcgtcc attaatgcgt caaatgattg cacaatttta cctggaacaa atgattcata
    11161 tggttcataa aaatcacgcg tcgtaatata atcttctaaa tcaaatgcat aga
//
LOCUS       DAKTIO010000089        11022 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.089, whole genome shotgun sequence.
ACCESSION   DAKTIO010000089 DAKTIO010000000
VERSION     DAKTIO010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11022
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.089"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            166..2355
                     /locus_tag="O0293_001693"
     CDS             166..2355
                     /locus_tag="O0293_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HCY0280579.1"
                     /translation="MNNEYPYSADEVLHKAKSYLSADEYEYVLKSYHIAYEAHKGQFR
                     KNGLPYIMHPIQVAGILTEMRLDGPTIVAGFLHDVIEDTPYTFEDVKEMFNEEVARIV
                     DGVTKLKKVKYRSKEEQQAENHRKLFIAIAKDVRVILVKLADRLHNMRTLKAMPREKQ
                     IRISRETLEIYAPLAHRLGINTIKWELEDTALRYIDNVQYFRIVNLMKKKRSEREAYI
                     ETAIDRIRTEMDRMNIEGDINGRPKHIYSIYRKMMKQKKQFDQIFDLLAIRVIVNSIN
                     DCYAILGLVHTLWKPMPGRFKDYIAMPKQNLYQSLHTTVVGPNGDPLEIQIRTFDMHE
                     IAEHGVAAHWAYKEGKKVSEKDQTYQNKLNWLKELAEADHTSSDAQEFMETLKYDLQS
                     DKVYAFTPASDVIELPYGAVPIDFAYAIHSEVGNKMIGAKVNGKIVPIDYILQTGDIV
                     EIRTSKHSYGPSRDWLKIVKSSSAKGKIKSFFKKQDRSSNIEKGRMMVEAEIKEQGFR
                     VEDILTEKNIQVVNEKYNFANEDDLFAAVGFGGVTSLQIVNKLTERQRILDKQRALNE
                     AQEVTKSLPIKDNIITDSGVYVEGLENVLIKLSKCCNPIPGDDIVGYITKGHGIKVHR
                     TDCPNIKNETERLINVEWVKSKDATQKYQVDLEVTAYDRNGLLNEVLQAVSSTAGNLI
                     KVSGRSDIDKNAIINISVMVKNVNDVYRVVEKIKQLGDVYTVTRVWN"
     gene            2367..2819
                     /locus_tag="O0293_001694"
     CDS             2367..2819
                     /locus_tag="O0293_001694"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCY0280580.1"
                     /translation="MKVVVQRVKEASVTNDTLNNQIKKGYCLLVGIGQNSTEQDADVI
                     AKKIANARLFEDDNNKLNFNIQQMNGEILSVSQFTLYADVKKGNRPGFSNSKNPDQAV
                     KIYEYFNDALRAYGLTVKTGEFGTHMNVSINNDGPVTIIYESQDGKIQ"
     gene            2816..3691
                     /locus_tag="O0293_001695"
     CDS             2816..3691
                     /locus_tag="O0293_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCY0280581.1"
                     /translation="MKKIEAWLSKKGLKNKRTLIVVIAFVLFIIFLFLLLNSNSEDSG
                     NITITENAELRTGPNAAYPVIYKVEKGDHFKKIGKVGKWIEVEDTSSNEKGWIAGWHT
                     NLDIVADNTKEKNPLQGKTIVLDPGHGGSDQGASSNTKYKSLEKDYTLKTAKELQRTL
                     EKEGATVKMTRTDDTYVSLENRDIKGDAYLSIHNDALESSNANGMTVYWYHDNQRALA
                     DTLDATIQKKGLLSNRGSRQENYQVLRQTKVPAVLLELGYISNPTDETMIKDQLHRQI
                     LEQAIVDGLKIYFSA"
     gene            4152..5414
                     /locus_tag="O0293_001696"
     CDS             4152..5414
                     /locus_tag="O0293_001696"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCY0280582.1"
                     /translation="MIKIPRGTQDILPEDSKKWRYIENQLDELMTFYNYKEIRTPIFE
                     STDLFARGVGDSTDVVQKEMYTFKDKGDRSITLRPEGTAAVVRSYIEHKMQGNPNQPI
                     KLYYNGPMFRYERKQKGRYRQFNQFGVEAIGAENPSVDAEVLAMVMHIYQSFGLKHLK
                     LVINSVGDMASRKEYNEALVKHFEPVIHEFCSDCQSRLHTNPMRILDCKVDRDKEAIK
                     TAPRITDFLNEESKAYYEQVKAYLDDLGIPYIEDPNLVRGLDYYTHTAFELMMDNPNY
                     DGAITTLCGGGRYNGLLELLDGPSETGIGFALSIERLLLALEEEGIELDIEENLDLFI
                     VTMGDQADRYAVKLLNHLRHNGIKADKDYLQRKIKGQMKQADRLGAKFTIVIGDQELE
                     NNKIDVKNMTTGESETIELDALVEYFKK"
     gene            5430..7196
                     /gene="aspS"
                     /locus_tag="O0293_001697"
     CDS             5430..7196
                     /gene="aspS"
                     /locus_tag="O0293_001697"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCY0280583.1"
                     /translation="MSKRTTYCGLVTEAFLGQEITLKGWVNNRRDLGGLIFVDLRDRE
                     GIVQVVFNPAFSEEALKIAETVRSEYVVEVQGTVTKRDPETVNPKIKTGQVEVQVTNI
                     KVINKSETPPFSINEENVNVDENIRLKYRYLDLRRQELAQTFKMRHQITRSIRQYLDD
                     EGFFDIETPVLTKSTPEGARDYLVPSRVHDGEFYALPQSPQLFKQLLMISGFDKYYQI
                     VKCFRDEDLRADRQPEFTQVDIEMSFVDQEDVMQMGEEMLKKVVKEVKGVEINGAFPR
                     MTYKEAMRRYGSDKPDTRFEMELIDVSQLGRDMDFKVFKDTVENDGEIKAIVAKGAAE
                     QYTRKDMDALTEFVNIYGAKGLAWVKVVEDGLTGPIGRFFETENVETLLTLTGAEAGD
                     LVMFVADKPNVVAQSLGALRVKLAKELGLIDETKLNFLWVTDWPLLEYDEDAKRYVAA
                     HHPFTSPKEADIAKLGTAPEEAEANAYDIVLNGYELGGGSIRIHDGELQEKMFEVLGF
                     TKEQAQEQFGFLLDAFKYGAPPHGGIALGLDRLVMLLTNRTNLRDTIAFPKTASATCL
                     LTNAPGEVSDKQLEELSLRIRH"
     gene            7274..7464
                     /gene="ssrS"
                     /locus_tag="O0293_001698"
     ncRNA           7274..7464
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="O0293_001698"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            7529..7657
                     /locus_tag="O0293_001699"
     CDS             7529..7657
                     /locus_tag="O0293_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280584.1"
                     /translation="MGFIAFNKCFLVEFWENNQSDNAKYKILSYYVLMYKSVKEVK"
     gene            7657..8430
                     /locus_tag="O0293_001700"
     CDS             7657..8430
                     /locus_tag="O0293_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HCY0280585.1"
                     /translation="MKHQFSRNELAIGQQGLDLLKQQTVVILGVGGVGSFAAEALART
                     NIGHIILIDKDDVDITNVNRQLHALTTTVGQSKVSLMEERIKLINPDCKVTPLHMFYT
                     EETYEEIFDNYDIDYFVDASDTIMYKVHLMKECLDRGIKVISSMGAANKTDPTRFTIS
                     DISKTHTDPMAKIIRQKLKKLGIRKGIPVVFSDESPIVIREDVKDIVGDKNAINRKGQ
                     MPPSSNAFVPSVVGLICASYVVNDVLKDIPVRRIKDKGQ"
     gene            complement(8591..9865)
                     /locus_tag="O0293_001701"
     CDS             complement(8591..9865)
                     /locus_tag="O0293_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HCY0280586.1"
                     /translation="MSTEPLASRMRPKNIDEIISQQHLVGPRGIIRRMVDTKKLTSMI
                     FYGPPGIGKTSIAKAISGSTQYKFRQLNAVTNTKKDMQLVVEEAKMSGQVILLLDEIH
                     RLDKAKQDFLLPHLENGKIVLIGATTSNPYHAINPAIRSRAQIFELYPLNDEDVRQAL
                     TRAIEDDENGLKTYQPKIDEDAMTYFSTQSQGDVRSALNALELAVLSADNDKDGYRHV
                     TLQDAKDCLQKGAFVSDKDGDMHYDVMSAFQKSIRGSDVNAALHYLARLIEAGDLPTI
                     VRRLLVISYEDIGLASPNAGQRTLAAIESAERLGLPEARIPLSQAVIELCLSPKSNSA
                     MSAIDSALSDIRNGHVGQIPNHLKDGHYQGAKDLGRSIGYKYPHQYVNGYVSQQYLPD
                     KLKNKIYYEPKTTSKSEQQLKEIYNNLLKQRP"
     gene            9950..10372
                     /locus_tag="O0293_001702"
     CDS             9950..10372
                     /locus_tag="O0293_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCY0280587.1"
                     /translation="MKISTKGRYGLTLMISLAKKEGQGCISLKSIAEENNLSDLYLEQ
                     LVGPLRNAGLIRSVRGAKGGYQLRVPAEEISAGDIIRLLEGPITFVESIESEPPAQKQ
                     LWIRMRDAVRDVLDNTTLKYLAEYVDTSEDLDGYMFYI"
     gene            10472..10654
                     /locus_tag="O0293_001703"
     CDS             10472..10654
                     /locus_tag="O0293_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCY0280588.1"
                     /translation="MADESKFDQFKGNVKETVGNVTDNKELEKEGQQDKVIGKAKEVV
                     ENAKNKITDAIDKLKK"
     gene            10694..10840
                     /locus_tag="O0293_001704"
     CDS             10694..10840
                     /locus_tag="O0293_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280589.1"
                     /translation="MSFMDKAKDAVEKFKNSDNEQVKNVKDKINEYTGSNNEEKKENE
                     DKEK"
BASE COUNT         3960 a         1541 c         2163 g         3358 t
ORIGIN      
        1 caaattaaac tcgctcaagt aaaattaata ttacgattaa tgacgacagg ataaatattt
       61 atcgtcgacg gacgtatgat tggtgtggga caaatactat tcaacaagag tacctaaatc
      121 attgtttaag gcgaagtaat aaatatgaat ggggtgtatc atataatgaa caacgaatat
      181 ccatatagtg cagacgaagt tcttcacaaa gcaaaatcat atttgtcagc agatgaatat
      241 gagtatgttt taaaaagcta tcatattgct tatgaagcac ataaaggtca gttccgaaaa
      301 aacggattac catacattat gcatcctata caagttgcag gtattttaac agaaatgcga
      361 ttagacggac cgacgattgt cgcaggtttt ttgcatgatg taattgaaga tacaccgtat
      421 acatttgaag atgtaaaaga aatgttcaat gaagaagttg ctcgaattgt tgatggtgtg
      481 acgaagctta aaaaagtaaa ataccgctca aaagaagaac aacaagctga aaatcatcgc
      541 aagttattta ttgcgattgc caaagatgta cgcgtaattt tggtgaaatt agcagacaga
      601 ttacataata tgcgtacctt gaaagccatg ccgcgcgaaa aacaaattag aatttctcga
      661 gaaacattag aaatttatgc accattagca catcgtcttg gtattaatac aatcaaatgg
      721 gaactagaag atacggctct tcgttatatt gataatgtgc aatattttag aatagtcaat
      781 ttaatgaaga agaaacgtag tgaacgtgaa gcgtatatcg aaacggctat tgatagaata
      841 cgtactgaaa tggaccgaat gaatatcgaa ggcgatataa atggtagacc taaacatatt
      901 tacagtattt atcggaaaat gatgaagcag aaaaaacaat ttgatcaaat ttttgatttg
      961 ttggcgatac gtgttattgt caattctatt aatgattgtt atgcgatact tgggttggtg
     1021 catacgttat ggaaaccgat gccaggacgt tttaaagatt atattgcaat gcctaaacaa
     1081 aatttgtatc agtcattgca tactacagta gtaggcccaa atggagaccc gctcgaaatc
     1141 caaatacgaa cgtttgatat gcacgaaatt gctgagcatg gtgttgcagc acactgggct
     1201 tacaaagaag gtaaaaaagt aagtgaaaaa gatcaaactt atcaaaataa gttaaattgg
     1261 ttaaaagaat tagctgaagc ggatcataca tcgtctgacg ctcaagaatt tatggaaacc
     1321 ttaaaatatg acttacagag tgacaaagta tacgcattta ccccagcgag tgatgttatt
     1381 gagttgccat atggtgctgt gccgattgat tttgcttatg cgattcacag tgaagtaggt
     1441 aataagatga ttggtgccaa ggtgaatggc aaaattgtac caattgacta tattttacaa
     1501 acaggcgata ttgttgaaat acgtactagt aaacattcat atggaccaag tcgtgattgg
     1561 ttgaaaattg ttaaatcgtc tagtgccaaa ggtaaaatta aaagtttctt caaaaaacaa
     1621 gatcgttcat ctaatattga aaaaggccga atgatggttg aagctgaaat aaaagagcaa
     1681 ggatttagag tcgaagatat tttgacagag aaaaatattc aggttgttaa tgaaaaatat
     1741 aactttgcaa atgaagatga tttattcgca gctgtaggat ttggcggcgt gacatcctta
     1801 cagattgtta ataaattaac tgaaagacaa cgtattttag ataaacaacg tgctttaaat
     1861 gaagcacaag aagttacgaa atcattgcct attaaagaca acatcattac tgatagtggt
     1921 gtctatgtag aaggtttaga aaatgtactt atcaagttgt caaaatgttg taatcctata
     1981 ccaggtgatg atattgtagg ttatatcacc aaaggtcacg gtattaaagt acatcgcact
     2041 gattgcccaa atattaagaa cgaaactgaa cgactaatta atgttgaatg ggtaaaatca
     2101 aaagacgcaa ctcaaaaata tcaggttgat ttagaggtaa ctgcgtatga ccgaaatggc
     2161 ttgttgaatg aagtactaca agctgttagc tcgacagccg gcaatttaat taaagtttca
     2221 ggacgttcag atattgataa aaatgcaata ataaatatta gtgtcatggt gaaaaacgtg
     2281 aatgatgttt atcgtgtggt agaaaagatc aaacaacttg gtgatgttta tacagtaaca
     2341 agagtttgga actagaggtg caaaatatga aagtagttgt acaaagagtt aaagaagcat
     2401 cggtgacgaa tgatacatta aataatcaaa tcaaaaaagg atattgttta ttagtcggta
     2461 tcggtcagaa ctctacagag caagatgcag atgtaattgc aaagaaaatt gctaatgcaa
     2521 gattatttga agatgacaat aataaattaa actttaatat ccaacaaatg aatggtgaaa
     2581 tactatcagt ttcacaattt actctctatg cagatgtaaa aaaaggtaac cgtccaggtt
     2641 tctcaaattc taaaaatcct gatcaagcgg taaaaattta tgagtatttt aatgatgcgc
     2701 tacgagcgta tggtcttact gtgaaaacag gtgaatttgg aacacacatg aatgttagca
     2761 taaataatga tggtccagtc actattattt atgaaagtca ggacggcaaa attcaatgaa
     2821 aaaaatagag gcatggttat ctaaaaaggg tcttaaaaat aaacgtactc taatagtagt
     2881 gattgccttt gtcttattta tcatcttttt atttttattg ctgaatagca atagtgaaga
     2941 tagtgggaac atcacgataa ctgaaaatgc tgaattacgt acaggtccaa acgctgcgta
     3001 tccagtcata tataaagttg aaaaaggtga ccattttaaa aagattggta aagtaggtaa
     3061 atggattgaa gttgaagata catccagtaa tgaaaaaggt tggatagctg gatggcacac
     3121 aaatttagat attgtcgcgg ataatacgaa ggagaaaaat cctttgcaag gtaaaacaat
     3181 agtgcttgat cctggtcatg gaggtagtga ccagggtgct tcaagcaata ctaaatataa
     3241 aagtttagaa aaagattata cgttgaaaac agcaaaagaa ttgcagcgta ctttagaaaa
     3301 agaaggcgca actgttaaga tgacaagaac agacgataca tatgtttcac tagaaaatcg
     3361 tgatatcaaa ggcgatgcct atttgagtat acataatgat gcgttagaat catctaatgc
     3421 aaatggaatg acagtttatt ggtatcatga taatcaaaga gctttagcag atacgttaga
     3481 cgctacgatt cagaagaaag gtctactttc taatcgcggt tcaagacaag aaaattatca
     3541 agtgttaaga caaacaaaag ttcctgctgt tttattagaa ttaggttata ttagtaaccc
     3601 aactgatgaa acgatgatta aagatcaatt acatagacaa attttagaac aagcaattgt
     3661 tgatggcctt aaaatttatt tttctgcgta gggcttgcaa aaatatgtga aagtagttat
     3721 cattgatatt gaattttata actaaaaccg ttagtattct tgaaatggta aatgaaatag
     3781 gtagcaatct aactaagatt gtgtaggaat ataatccata gactgaaaga ttatgctgag
     3841 tagtttatat acattgaaca caagaagagg tgctttatga aaagtaaagc cgttaaacgt
     3901 acgttaaacg ttttgagtgg gtttattaaa tgcacgctta taaaaagtaa tgatgattac
     3961 aattaggcat gttttttaaa ccaatgaggg tggcaacacg gatattcgtc ccttgtgcag
     4021 tttcaggctg tgcaagggct ttttcttttg cttttaagat tatgatttat cgtgcaaagt
     4081 taagtggtcg tatatagttt tagttttaaa aaggtaatta aataaaatag tttgccgagg
     4141 gagatgtcaa aatgattaaa atacctagag ggacgcagga tattttacct gaagattcaa
     4201 agaaatggcg ttacattgaa aatcaattag atgaattaat gacattttat aattataaag
     4261 aaataagaac accaattttt gaaagtacag atctttttgc aagaggtgtt ggtgattcaa
     4321 ccgatgtcgt acaaaaagaa atgtatacat ttaaagataa aggcgataga agtattacat
     4381 taagacctga gggaacagct gcagttgtgc gttcatatat tgaacataaa atgcaaggta
     4441 atccaaacca accaattaaa ctttattaca atggaccgat gtttagatat gaacgtaagc
     4501 aaaaaggacg ctatcgtcaa tttaatcaat ttggtgtaga agctattggt gctgaaaatc
     4561 ctagcgtaga tgcagaagta ttagctatgg ttatgcatat ttatcaatca tttggattaa
     4621 aacatttaaa gcttgttatt aatagtgtag gggatatggc gtctcgaaaa gaatataacg
     4681 aagcgttagt gaaacacttt gaaccagtaa ttcatgaatt ttgttcagat tgtcaatcac
     4741 gtttgcatac aaatccgatg cgaattttgg attgtaaagt agaccgtgat aaagaagcga
     4801 ttaagactgc acctagaatc actgatttct taaatgagga atctaaggca tattatgaac
     4861 aagtaaaagc ttatttagat gatttaggta ttccatatat tgaagatcct aacttagttc
     4921 gtggattgga ttattataca catacagcat ttgaattaat gatggataac cctaactatg
     4981 atggtgccat tacaacgctt tgtggtggtg gccgttataa tggtttatta gaattgctag
     5041 atggtccaag tgaaacaggt attggttttg cgctaagtat agaacgatta ttgcttgcac
     5101 ttgaagaaga aggtatcgaa ttagatattg aagaaaactt agatttattc attgttacaa
     5161 tgggtgatca agcagatcga tatgctgtga agctattaaa tcatttgaga cataatggta
     5221 ttaaagcaga taaagactat ttacagcgta aaattaaagg acaaatgaaa caagcagacc
     5281 gtttaggtgc caagtttaca atcgttattg gtgatcaaga attagaaaat aataaaatcg
     5341 atgttaaaaa tatgacaact ggtgaatctg aaacaattga attagacgca ttagtcgaat
     5401 attttaagaa gtagagaggg cgttaaaata tgagtaagag aacaacttat tgtggattag
     5461 ttactgaggc atttttagga caagaaatta cattaaaagg atgggttaac aatcgtcgtg
     5521 accttggtgg attgattttc gttgatttaa gagatagaga aggaattgta caagtcgtgt
     5581 ttaatcctgc attttcagaa gaggcattga aaattgctga aacagtacgt tctgaatatg
     5641 ttgtagaagt tcaaggtaca gttacgaagc gtgaccctga aacagttaat cctaaaatta
     5701 aaactggcca agttgaagta caagttacaa atattaaagt gattaataaa tctgagacac
     5761 caccattttc tataaatgaa gaaaatgtta acgttgatga aaatattcga ttaaaatacc
     5821 gttatttaga tttacgtcgt caagagttag cgcaaacatt taaaatgaga catcaaatta
     5881 cacgttctat tcgtcaatat ttggatgatg aagggttctt tgacatcgaa acaccagtac
     5941 taacgaagtc aacacctgag ggtgcacgtg actatttagt accatctcgt gttcatgatg
     6001 gtgaatttta tgcattacca caatcaccac aattatttaa gcaattattg atgattagtg
     6061 gatttgacaa atactaccaa atcgtaaaat gcttccgtga cgaagattta cgtgcagatc
     6121 gtcaacctga atttacacaa gtcgatattg aaatgagttt tgtagaccaa gaagatgtga
     6181 tgcaaatggg tgaagaaatg cttaaaaaag ttgttaaaga agttaaaggc gttgaaatta
     6241 atggcgcttt cccacgcatg acatataaag aagcgatgcg tcgctatggt tctgataaac
     6301 cagatacacg ttttgaaatg gaattaattg acgtttctca attaggacgt gatatggact
     6361 ttaaagtatt taaagatact gttgaaaatg atggtgaaat taaagcaatt gtcgctaaag
     6421 gtgcagctga acaatatact cgtaaagata tggatgcttt aacagaattt gtaaacatct
     6481 atggtgctaa aggattagcg tgggttaaag ttgtggaaga tggtttgaca ggtccaattg
     6541 gacgtttctt tgaaacagaa aatgttgaaa cattacttac attaactggt gctgaagctg
     6601 gtgacttagt aatgtttgtt gcagacaaac caaatgtcgt tgcacaaagt ttaggtgcat
     6661 tacgtgtcaa attagctaaa gaattaggtt taatcgatga aacaaaatta aacttcttat
     6721 gggtgacaga ttggccatta ttagaatatg atgaagatgc gaaacgttac gttgcagcac
     6781 atcatccatt tacatctcca aaagaagctg atattgctaa gcttggcact gcgccagaag
     6841 aagctgaggc aaatgcttat gacatagtat taaatggtta tgaattaggt ggcggttcaa
     6901 tcagaattca tgatggtgag ttacaagaaa aaatgttcga agttcttgga tttactaaag
     6961 aacaagcaca agaacagttc ggcttcttac tagatgcatt taaatatggt gcaccaccac
     7021 atggcggtat tgcattagga ttagaccgat tagtcatgtt attgactaat agaacaaact
     7081 tgagagatac aatcgcattc cctaaaacag catctgctac atgtttatta acgaatgcgc
     7141 ctggtgaagt ttctgataaa caattagaag aactttcttt gcgaattcgt cactaaattt
     7201 taaaaaatca ataaactttg gtttgttata atttcattaa ttttaattat gtggtaatat
     7261 aacatcataa gatagtcatc tgagttgttc gtaagtttgc tttttaattt gggcctaaca
     7321 ctctttgatc aagggagccc aatgggtttt cttgcagcgt acacgcctta tatgaaggac
     7381 gtacaaaaca agaaacaggg cacccacctg tatataacag gccgaatgat caagctatta
     7441 taactacggc aatacggact ctatcgatac gcaagacttt gtcttgcgta tttttatgcg
     7501 tttctttaag tttttaataa agaattagtt gggttttata gcgtttaata aatgtttcct
     7561 cgttgaattt tgggaaaata atcaaagtga taatgctaaa tataaaattt taagttacta
     7621 tgtacttatg tataaatcag tgaaagaggt aaaataatga aacatcaatt ttcaagaaat
     7681 gaactagcga ttggacaaca aggtttagat ttattgaagc aacaaactgt tgtcatactt
     7741 ggcgttggag gtgttggttc atttgctgct gaagcattag caagaacgaa tattggtcat
     7801 attatattaa tagataaaga tgatgttgat attacaaatg taaacagaca attacacgct
     7861 ttaactacaa cggtaggaca aagtaaagtt tctttgatgg aagaacgtat taaattaatt
     7921 aatccagatt gtaaagtcac gcccttacat atgttttata cagaagagac atatgaagaa
     7981 attttcgata actacgatat agattatttt gtagatgcaa gtgatacaat aatgtacaaa
     8041 gtacacttaa tgaaagagtg tcttgaccga gggattaaag tgatttccag tatgggtgct
     8101 gcaaataaaa cagatccaac acgttttaca atttcagata tctctaaaac acataccgat
     8161 ccaatggcca aaattattcg tcaaaaattg aaaaaactag gaattcgtaa agggattcca
     8221 gttgtatttt cagatgaaag tccaattgtc ataagagaag atgtaaaaga tatagttgga
     8281 gataaaaatg ctatcaatcg aaaaggacaa atgccacctt cttcaaatgc ctttgtgcca
     8341 agtgttgttg gattaatttg tgcaagttat gtggtgaatg acgtattaaa agatattcca
     8401 gttcgtcgca ttaaagacaa agggcaataa ttcattttga aagggataat tttcaacgta
     8461 aggcaagtgt aaccacacca taaaactaaa actgactagt tcgcaagcat agtagaatat
     8521 gcctcgtgta ctagtcagtt ttgatttgat tatagctaaa atatcaaagg taatcgattt
     8581 aatcttattt ttacggcctt tgtttaagta agttgttata tatttctttg agttgttgtt
     8641 cacttttaga tgtcgttttt ggttcgtaat aaattttgtt ttttagttta tcaggtaaat
     8701 attgctgtga aacatagcca ttaacatatt ggtgtggata tttgtaacca atagatcggc
     8761 ctagatcttt agcaccttga taatgtccat cttttaaatg gtttggaatt tggcccacat
     8821 gaccgtttct aatatcggac aatgcactat caatggcact cattgctgaa tttgacttag
     8881 gtgataagca tagttcgatt actgcttggc ttagtggaat tctagcttct ggtaaaccta
     8941 gacgttctgc tgattcaata gcagcaagtg ttctctgacc agcattaggt gaggctaagc
     9001 ctatatcctc atagctaatt acaagtaatc gtcgaactat tgtaggtaaa tctccagctt
     9061 caattaatcg tgctaaataa tgtaaagcgg cattgacgtc gctaccacgg atagattttt
     9121 ggaaagcgct cataacatcg taatgcatgt caccatcctt gtcacttaca aatgcacctt
     9181 tttgtaaaca gtctttagca tcttgcaatg taacatgtcg ataaccgtct ttgtcattat
     9241 ctgcgcttaa tacagctaat tccaatgcat ttaacgcact acgaacatca ccttggcttt
     9301 gtgtagaaaa gtaggtcatg gcatcttcat caattttggg ttgatatgtt ttcaaaccat
     9361 tctcatcatc ttctattgca cgagttaacg cttggcgcac atcttcgtca tttaaaggat
     9421 ataactcgaa aatttgcgct cttgaacgaa tcgctggatt gatagcatga taaggatttg
     9481 aagttgtagc accgatcaag acgattttgc cattttctaa atgaggtaat aaaaagtctt
     9541 gtttagcttt atctagtcga tgtatttcat ctaataacaa gataacttga ccagacattt
     9601 tagcttcttc aacaacaagt tgcatatctt ttttagtgtt agttacagca ttcaattgtc
     9661 tgaatttata ttgcgtactg cccgaaattg ctttggcaat acttgttttg cctataccag
     9721 gtggaccata aaaaatcatt gaagttaatt tttttgtatc aaccattctt ctgataatgc
     9781 ctcttggtcc aactaaatgt tgttgggaaa tgatttcatc tatatttttt gggcgcattc
     9841 tcgatgctaa tggttctgta ctcacatttg tcacaccttt cttatactat cttaactcaa
     9901 aaaatgctaa aatgggaata tcaatattcg aattaagtga ggttatacaa tgaaaatttc
     9961 tactaaaggg agatatggac ttacattgat gatttctctt gctaaaaaag aggggcaagg
    10021 atgtatatca ttaaagtcaa ttgctgaaga aaataatttg agtgatttat atttagaaca
    10081 gcttgtaggt cctttaagaa atgcggggtt aattcgaagt gtacgcggtg ctaaaggtgg
    10141 ataccaatta agagtgccag cggaagaaat ctcagcaggg gatattataa gactgttaga
    10201 aggtccaatt acatttgttg aaagtattga atcagaacca cctgcgcaaa aacaactatg
    10261 gattcgcatg agagatgcag tgagagatgt tttagataat acaacattga aatatttagc
    10321 ggaatatgta gatacaagtg aagatttaga cggatacatg ttttatattt aatcgtataa
    10381 atactatact ataacataaa aacttcatat tataatgttt agcgaacctc cttagtggta
    10441 tataaatata tacatccaag gaggtttcac tatggcagac gaaagtaagt tcgatcaatt
    10501 taaaggaaat gttaaagaaa cagtaggtaa cgttactgat aacaaagaat tagaaaaaga
    10561 aggtcagcaa gataaagtga ttggtaaagc aaaagaagtt gttgaaaatg ctaaaaacaa
    10621 aataactgat gcaattgata aacttaaaaa ataagttcaa ttaaactaat caaatataag
    10681 aggagcgatt aatatgagtt ttatggataa agctaaagac gcagtagaaa aatttaaaaa
    10741 tagcgataat gaacaagtta aaaatgtaaa agataagata aatgaatata caggatcgaa
    10801 taacgaagag aaaaaagaaa atgaagataa agagaaataa atttctttaa attttggata
    10861 tgcttttcaa tattttgaaa gcgccccatc aaataaacac ttacacagtg taaagttgaa
    10921 ggggcgcttt catatttgca ttagaatttt aagtgaattt gaatcattac taaaataaat
    10981 gattgaacaa taaagacatc tttcattcaa aatgctgtta ct
//
LOCUS       DAKTIO010000090        10779 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.090, whole genome shotgun sequence.
ACCESSION   DAKTIO010000090 DAKTIO010000000
VERSION     DAKTIO010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10779
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.090"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            343..2307
                     /locus_tag="O0293_001705"
     CDS             343..2307
                     /locus_tag="O0293_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HCY0280590.1"
                     /translation="MTQITEKELKKKYLDLLSQNFDTPEKLATEIINLESILELPKGT
                     EHFVSDLHGEYEAFQHVLRNGSGNVRAKINDIFKERLSTKELNDLTALVYYPEDKLKL
                     IKSDFQNCGQLNVWYITTIEHLIELIKYCSSKYTRSKLRKALPKQYVYIIEELLYKSN
                     EYQNKKSYYETLVNQVIELKQADDLIIGLAYSVQRLVVDHLHVVGDIYDRGPQPDKIM
                     DTLINYHSLDIQWGNHDVLWVGAYAGSKVCLANLLRICARYDNLDIIEDAYGINLRPL
                     LTLAEKYYDADNPAFKPKKRPDKHERLTQREESQITKIHQAIAMIQFKLEIPIIKRRP
                     NFEMEERLVLEKVNYDTNEITVYGNTYPLKDTCFQTVNRDNPAELLPEEEEVMNKLLL
                     SFQQSEKLRRHMSFLMRKGSLYLPYNGNLLIHGCIPVDENGEMESFEIDGHTYSGQEL
                     LDVFEYHVRKSFDEKENTDDLSTDLVWYLWTGKYSSLFGKRAMTTFERYFIADKASHK
                     EEKNPYYHLREDVNMVRKMLSDFGLNPDEGRIINGHTPVKEINGEDPIKADGKMLVID
                     GGFSKAYQSTTGIAGYTLLYNSFGMQLVAHQQFNAKEKILSEGIDELSIKRVVDKELQ
                     RKKIRDTNIGKDLQAQIDILKMLMHDRYLD"
     gene            2651..3730
                     /locus_tag="O0293_001706"
     CDS             2651..3730
                     /locus_tag="O0293_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280591.1"
                     /translation="MRILKESIIVAFAFVGVVVGAGFATGQEIFQFFTSHGAYSISGI
                     IVTGLLITLGGMVVMHTGHHLKSRNHSDSINYFLYPSIARGFDIILTMFMLSLAIIMT
                     AGGASTIHQSFNLPYWLSALILVAFILATLFLKFDRLIAVLGGVTPFLIAIVIMIAVY
                     YFTTSHLDFTAANNDAQIHKQKSLSPGWWFDAINYASLQIAAAFSFLSVMGSKVKYRD
                     STLYGGLIGGLIITFLLMMINLGLISQFDKIKHVDLPTLKLATQMSPSIGIIMSVIMI
                     LVIYNTVVGLMYAFASRFSVPFSRRYFIIIITMAVITYISTFIGFISLIGKVFPIMGL
                     FGFILLIPVLYKGLIKRITGKSHID"
     gene            complement(3835..4428)
                     /locus_tag="O0293_001707"
     CDS             complement(3835..4428)
                     /locus_tag="O0293_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0280592.1"
                     /translation="MEHIFREGQNGAPTLILLHGTGGDEFDLLPLGEALNENYHLLSI
                     RGQVSENGMNRYFKRLGEGVYDEEDLAFRGQELLTFIKEAAERYDFDIEKAVLVGFSN
                     GSNIAINLMLRSEAPFKKALLYAPLYPVEVTSTKDLSDVSVLLSMGKHDPIVPLAASE
                     QVINLFNTRGAQVEEVWVKGHEITETGLTAGQQILGK"
     gene            complement(4450..5418)
                     /locus_tag="O0293_001708"
     CDS             complement(4450..5418)
                     /locus_tag="O0293_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HCY0280593.1"
                     /translation="MINNHELLGIHHVTAMTDDAERNYKFFTEVLGMRLVKKTVNQDD
                     IYTYHTFFADDVGSAGTDMTFFDFPNITKGQAGTNSITRPSFRVPNDDALTYYEQRFD
                     EFGVKHEGIQELFGKKVLPFEEVDGQVYQLISDELNEGVAPGVPWKNGPVPVDKAIYG
                     LGPIEIKVSYFDDFKNILETVYGMTTIAHEDNVALLEVGEGGNGGQVILIKDDKGPAA
                     RQGYGEVHHVSFRVKDHDAIEAWATKYKEVGINNSGIVNRFYFEALYARVGHILIEIS
                     TDGPGFMEDEPYETLGEGLSLPPFLENKREYIESEVRPFNTKRQHG"
     gene            complement(5496..5930)
                     /locus_tag="O0293_001709"
     CDS             complement(5496..5930)
                     /locus_tag="O0293_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0280594.1"
                     /translation="MDRTKQSLNVFVGMNRALDTLEQITKEDVKRYGLNITEFAVLEL
                     LYNKGPQPIQRIRDRVLIASSSISYVVSQLEDKGWITREKDKDDKRVYMACLTEKGQS
                     QMADIFPKHAETLTKAFDVLTKDELTILQQAFKKLSAQSTEV"
     gene            6157..6441
                     /locus_tag="O0293_001710"
     CDS             6157..6441
                     /locus_tag="O0293_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCY0280595.1"
                     /translation="MSNLEIKQGENKFYIGDDENNALAEITYRFVDNNEINIDHTGVS
                     DELGGQGVGKKLLKAVVEHARENNLKIIASCSFAKHMLEKEDSYQDVYLG"
     gene            6612..7418
                     /locus_tag="O0293_001711"
     CDS             6612..7418
                     /locus_tag="O0293_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0280596.1"
                     /translation="MAFHDKTATQVTNIVLNVRDLDLMTTFYKNILGLSVKSSDDNTT
                     VLSVGTGGHTLTLHLLEDGRQTSPREAGLFHIAFLLPTTEDLANFLYFVAQKNMGIGA
                     GDHLVSEALYFNDPEGNGIEVYRDRPSSSWEWQNGKVKMDTLEVDSQTLLTHRTDEGW
                     QGMPAKGMIGHLHLKTHDLDAAYQCYIEQLGFQHVSDFPRALFMSTNHYHHHIAANTW
                     QSNQARQNNSQSYGLTHVDIYQPDASTHTFTAPEGFDITVHSNTDLVPEK"
     gene            complement(7538..8209)
                     /locus_tag="O0293_001712"
     CDS             complement(7538..8209)
                     /locus_tag="O0293_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCY0280597.1"
                     /translation="MSNMNQTIMDAFHFRHATKQFDPQKKVSKEDFETILESGRLSPS
                     SLGLEPWKFVVIQDQALRDELKAHSWGAAKQLDTASHFVLIFARKNVTSRSPYVQHML
                     RDIKKYEAQTIPAVEQKFDAFQADFHISDNDQALYDWSSKQTYIALGNMMTTAALLGI
                     DSCPMEGFSLDTVTDILANKGILDTEQFGLSVMVAFGYRQQEPPKNKTRQAYEDVIEW
                     VGPKE"
     gene            8479..9471
                     /locus_tag="O0293_001713"
     CDS             8479..9471
                     /locus_tag="O0293_001713"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCY0280598.1"
                     /translation="MTKIMFFGTRDYEKEMALNWGKKNNVEVTTSKELLSSATVDQLK
                     DYDGVTTMQFGKLENDVYPKLESYGIKQIAQRTAGFDMYDLDLAKKHNIVISNVPSYS
                     PETIAEYSVSIALQLVRRFPDIERRVQAHDFTWQAEIMSKPVKNMTVAIIGTGRIGAA
                     TAKIYAGFGATITAYDAYPNKDLDFLTYKDSVKEAIKDADIISLHVPANKESYHLFDK
                     AMFDHVKKGAILVNAARGAVINTPDLIAAVNDGTLLGAAIDTYENEAAYFTNDWTNKD
                     IDDKTLLELIEHERILVTPHIAFFSDEAVQNLVEGGLNAALSVINTGTCETRLN"
     gene            complement(9720..10520)
                     /locus_tag="O0293_001714"
     CDS             complement(9720..10520)
                     /locus_tag="O0293_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCY0280599.1"
                     /translation="MVKAIAVDMDGTFLDSKKTYDKLRFEAIFTELRNRDITFIAASG
                     NQYAKLKSIFGDRDMYFISENGAVIYNGNELYNYKSFNRQVFQQVVDYLNMKQSIDQL
                     VICGLKSAYILKHTSEAFKEDTRFYYHQLKEIDSLQQLPEDDYVKIAFNINRETHPNV
                     DEEVATQFSNDIKLVSSGNDSIDIIMPNMTKGQALKRLLDKWEMSPSELMAFGDANND
                     KDMLAFAKHSYVMENSHDEELFNIASAVAPSNDKQGVLTIIEQEVLKK"
BASE COUNT         3456 a         1991 c         1678 g         3654 t
ORIGIN      
        1 acatattcca tcgtaattca aaatcatttt caaatccctt cacccaaata atggtgcggg
       61 gattttttca tccaaatttt ggaaattcca cattttacat atcgtaattt tttaggaaac
      121 tagtgaatat aacaaatccc tcctctcatt tttaaaatag atatatcact tccccacttt
      181 tacttaacta aactgcaacg gttcctaata ccaaaatcct gccctctatt tttatcaatt
      241 caagcatact tattgaaaaa tgttaacgtt ttcttgataa tcattgtaag cgcatttatt
      301 ttataaacta acgtttgaaa tatactacag gagtgacacg taatgactca aattactgaa
      361 aaagaattaa aaaagaagta tttagattta ctatcccaaa attttgatac tccagaaaaa
      421 cttgcaactg aaattatcaa tttagaatca attttagaat tacctaaagg tacggaacat
      481 ttcgtcagcg atttacatgg tgaatatgaa gctttccaac acgtattacg caacggttct
      541 gggaacgtgc gagcgaaaat caatgatatt ttcaaagaga gactttcaac taaggagctt
      601 aatgacttaa ctgctcttgt ctactatcca gaagacaaat taaaattgat taaaagtgat
      661 ttccaaaatt gcggtcaact taatgtctgg tatatcacaa caatcgaaca tttaattgag
      721 ttaattaaat attgttcttc aaaatatacg cgttcaaaac tgcgtaaagc attgccaaaa
      781 caatacgttt atattattga ggaactactg tataaaagta atgaatatca aaataaaaaa
      841 tcatattacg aaacacttgt taaccaagta attgaactta aacaggcaga tgatttaatt
      901 attggactcg cttattccgt acaacgctta gtcgtcgatc atttacacgt tgtcggtgat
      961 atttatgatc gtggaccaca accagataaa attatggata cactgattaa ttatcattcc
     1021 ctagatattc aatggggtaa tcatgatgtg ctttgggttg gagcctatgc tgggtcaaaa
     1081 gtatgcttag caaacttact tcgaatttgt gcacgttatg ataatttaga tattatcgaa
     1141 gacgcttatg gcattaattt aagaccactg cttactttag ctgaaaaata ctatgacgca
     1201 gataatcctg cttttaagcc taaaaaaaga cctgacaaac acgaacgttt aactcaacgt
     1261 gaagaaagtc aaattactaa aattcatcaa gctattgcga tgattcaatt caagttagaa
     1321 ataccaatta ttaaacgtcg tccaaatttc gaaatggaag aacgtcttgt gcttgaaaag
     1381 gttaattatg atacaaatga aattacagtt tatggtaata catacccatt gaaagacaca
     1441 tgtttccaaa ctgtcaatcg tgataatcca gcagaattac tacctgaaga agaagaagtc
     1501 atgaataaac tattattgtc attccaacaa tctgaaaaat tacgtcgtca tatgtctttc
     1561 ttgatgcgta aaggctctct ttacttacca tataatggca atttactcat tcatggttgt
     1621 attccagttg atgaaaatgg tgagatggaa tcatttgaaa ttgatggtca tacttacagc
     1681 ggccaagaat tattagatgt gtttgagtat catgtccgta aatcatttga tgaaaaagaa
     1741 aatactgatg acttatcgac ggatttagtt tggtatttat ggactgggaa atattcgtca
     1801 ctatttggta aacgtgccat gactacgttt gagcgatact ttattgcaga taaagcttct
     1861 cataaagaag aaaagaatcc gtactatcat cttcgtgaag atgtgaatat ggttcgtaaa
     1921 atgctcagtg atttcggatt aaatccagat gaaggacgca ttattaatgg tcacacacca
     1981 gtgaaagaaa tcaatggcga agatcctatc aaggctgatg gaaagatgct tgtcattgat
     2041 ggtggctttt caaaagctta tcagtcaaca actggcattg caggctatac actattgtat
     2101 aattcattcg gtatgcaact cgttgcacac caacaattta acgctaaaga aaaaatactt
     2161 tccgaaggta tcgatgaact ttctataaag cgcgtcgtag ataaagaatt acaacgtaag
     2221 aagattcgtg ataccaatat tggtaaagat cttcaagctc aaatagatat tttgaaaatg
     2281 ttaatgcatg atcgttactt agactaaaga cgacatttga tgcatgtatc gtgcacttat
     2341 ttagtggtac tgtgtttacg cattggctac gcaaacacga tgataaatag gcgacctagc
     2401 tacaccgaaa aaaatcccct cactattgcg agtagtgagg ggattggtgt atctatgcta
     2461 attcctgtta actgtattat aacaacttta cagcaaaaaa atgcaaaatc aactaataaa
     2521 ttaatgaaat gaccgacacc ctcgcttcta ctccaattta acaaaatact atattagtac
     2581 tatatttttc attaacatgt atgttacatt ttctatatta cttaaaatta cgaagggaca
     2641 tttttaaatc atgcgtatct taaaagagtc cattattgtg gcatttgcct ttgttggtgt
     2701 tgtcgttggt gccggctttg ctactggtca agaaattttc cagtttttca caagtcatgg
     2761 cgcatatagc atttcaggca ttattgtaac aggactattg attactttag gtggaatggt
     2821 tgtcatgcat acaggtcatc atctaaagtc cagaaatcat tctgattcaa ttaactattt
     2881 cttatacccc tctattgcaa gaggttttga tattatttta acaatgttta tgttgtcttt
     2941 agctattatt atgactgcag gtggtgcgtc aaccattcat caaagtttca acttaccgta
     3001 ttggctgagc gcactcatat tagtcgcctt tattttagca acactgtttc taaaattcga
     3061 tcgtttaatt gctgtgcttg gcggtgttac cccattttta attgcgattg tcattatgat
     3121 tgcggtctac tatttcacaa caagtcatct tgattttact gccgctaata atgatgctca
     3181 gattcataag cagaaatcat tatcacctgg atggtggttt gatgcgatta actatgcaag
     3241 cttgcaaatt gctgctgcct tcagcttctt atcagtgatg ggtagtaaag ttaaatatcg
     3301 tgactcaacg ttatacgggg gcttgattgg cggtttaatc attacatttt tactcatgat
     3361 gattaatcta ggtttaattt ctcaattcga taaaattaaa cacgtagatc tacctacatt
     3421 aaaattagcg acacaaatgt ctccgtcaat tggtattatt atgtctgtca ttatgatact
     3481 tgtcatctac aatactgttg ttggattaat gtatgcattt gcgtcacgtt tcagcgttcc
     3541 attcagcaga cgttacttca tcattattat tacaatggct gtcatcactt atattagtac
     3601 atttatcggt ttcatttcat taattggaaa agtattccct attatgggat tgttcggttt
     3661 catcttactc atacctgtac tctataaagg tttaattaag cgtattaccg gcaaatctca
     3721 tatcgattaa aatatctata ctattgtaaa atttagtaca gcacttcaaa atcaaaaaag
     3781 cgggcagatg aaaagtaaaa atcttttcca tctgtccgct tcttaataga actgttattt
     3841 cccaagtatt tgttgaccag ccgttaatcc agtttctgta atttcatggc ccttcaccca
     3901 aacttcttcg acttgtgccc cacgtgtatt aaacaagtta atgacttgtt cacttgcagc
     3961 taatggcaca attggatcat gtttccccat agaaagcaac acactgacat ctgataaatc
     4021 ctttgttgac gttacttcaa ctgggtataa cggtgcatat aacaatgctt ttttaaatgg
     4081 tgcttctgaa cgcaacatta agttaatcgc tatatttgat ccatttgaaa atccaacaag
     4141 tactgctttt tcaatatcaa aatcataacg ttcagcagct tctttaatga acgtcaacaa
     4201 ttcttgtcca cgaaatgcca aatcttcttc atcataaaca ccttcaccaa gacgtttgaa
     4261 ataacggttc atcccatttt ctgaaacttg tcctctaata cttaacaagt gataattttc
     4321 attcaatgct tcgcctaacg gtaataaatc gaactcatca ccacctgtac catgcaataa
     4381 tattagtgtt ggcgcaccat tttgtccttc tctaaaaata tgttccatca caaatcctcc
     4441 tcattccaat taaccatgtt gacgcttcgt attaaaaggt ctaacttccg attcaatata
     4501 ttctctttta ttttctaaaa atggtggtaa ggataaccct tcgcctaatg tttcataagg
     4561 ttcatcttcc ataaatcctg gtccatctgt tgaaatttct attaaaatat gccccacacg
     4621 tgcatataat gcttcaaaat agaaacgatt aacgatgcct gagttattaa tacctacctc
     4681 tttatatttc gttgcccacg cttctattgc atcatgatct ttcacacgaa atgacacatg
     4741 atgtacctca ccataacctt gacgtgctgc tggcccttta tcatctttta ttaagattac
     4801 ctggccacca ttgcctcctt cgccaacttc aagtaatgcg acattatctt catgcgcaat
     4861 agttgtcata ccgtaaacag tctctaaaat atttttaaag tcgtcaaaat aacttacttt
     4921 aatttcaatg gggcctaatc cataaatcgc tttatctact ggaaccggtc cattcttcca
     4981 aggtacacca ggtgctaccc cttcatttaa ctcatctgaa attaattgat acacttggcc
     5041 atcgacttct tcaaatggca acactttttt accaaataat tcttgaatac cttcgtgttt
     5101 aacaccaaac tcatcaaagc gctgttcata atatgttaat gcgtcatcgt taggcactct
     5161 aaaagacggt cttgtaatgg aatttgttcc tgcctgccct tttgtaatat ttggaaaatc
     5221 aaagaacgtc atgtctgtac ctgccgaacc tacatcatct gcaaaaaaag tatgatacgt
     5281 ataaatatca tcttgattga ctgtcttttt aactaaacgc atgcctagta cttctgtaaa
     5341 aaatttataa ttacgttctg catcatctgt cattgcagta acatggtgaa tacctagtaa
     5401 ttcatgatta tttatcattt tatataccct ctttatattt ttagtgcatt tatctcgaaa
     5461 tcgagatact ttaatgtaaa tttttagtgc acgccttaca cttctgtaga ttgtgcactt
     5521 agtttcttaa acgcttgttg taagattgtt aattcatcct ttgttaacac atcaaacgct
     5581 tttgttaatg tctcagcatg cttagggaaa atatctgcca tttgactttg acctttttca
     5641 gttaaacaag ccatatatac acgtttatca tctttatcct tttcacgtgt aatccaacct
     5701 ttgtcctcta attgacttac aacatatgaa atgctgctac ttgcaattaa tacgcggtct
     5761 ctaatacgtt gaattggttg cggaccttta ttataaagca actcgagcac tgcaaattca
     5821 gtaatattta agccatatcg ctttacgtct tcttttgtaa tttgctctaa tgtgtctaac
     5881 gccctattca ttccgacaaa aacattgaga gattgtttcg ttcgatccat atgatcacca
     5941 acttttttat tttatttcga tttcgagata aattatatag ctgaatagat ttaatgtcaa
     6001 gatacgtgtt ttcatcttca tttcaaactg tgcttaataa aaacgactac atagatgtgg
     6061 caatgtcgtc acatgactgt catgcacctt tgacttcgtt taagttaaaa tagaataggg
     6121 aaatactata atcaatcatg ataggagttg atatgtatga gtaaccttga aatcaaacaa
     6181 ggcgagaaca aattctatat tggtgatgat gaaaataatg ctttagctga aatcacatac
     6241 cgttttgtgg ataataatga aattaacatt gatcatacag gcgtatctga tgaacttggt
     6301 ggtcaaggtg ttggcaaaaa actacttaaa gcagttgttg aacacgctcg agaaaataat
     6361 ttgaaaatta ttgcctcatg ttcatttgcc aaacatatgt tagaaaaaga agattcatat
     6421 caagatgtat atcttggtta aataaatttt gcgatggtgc aactgtacca tcgctttttt
     6481 gttcgcatca atataaaatt gcaaaacgac tagttatttt gcatttcaaa ctttatgcta
     6541 tcaattcatt ttcttttctg atatactttt tatactaact tatagaaaaa tagaaaaaga
     6601 ggcgatgcat aatggcattt cacgataaaa cagcaacaca agtgacaaac attgtactga
     6661 acgtaagaga cttagactta atgacaacat tttacaaaaa cattttagga ctatccgtta
     6721 aaagttctga cgataataca accgtattat ctgttgggac tggtggccat actctaacgt
     6781 tacatttatt agaagacggc cgtcagactt ccccacgtga agcagggctt tttcatatag
     6841 catttttatt accaactact gaggatctag ctaacttctt atatttcgtg gcacaaaaaa
     6901 atatgggcat cggcgctggt gatcatttag taagtgaagc tttatatttc aacgaccccg
     6961 aaggtaatgg tattgaagtc tatcgcgata gaccttcatc ttcatgggaa tggcaaaatg
     7021 gcaaagttaa aatggatact ttagaagttg atagccaaac cttattaaca catcgtactg
     7081 atgaaggttg gcaaggaatg ccagcaaaag gcatgattgg acacttacat ttaaaaacac
     7141 atgatttaga cgcagcatat caatgttaca ttgaacaatt aggattccaa catgtgtctg
     7201 attttccacg tgcgctattt atgtcaacga atcattatca ccatcatatt gctgctaata
     7261 cttggcaatc caatcaagca agacaaaaca attcacaaag ttatggatta acacatgttg
     7321 atatttatca acccgacgcc tcaacacata catttacggc accagaaggg tttgacatca
     7381 cagttcattc aaatactgac cttgttccag aaaaataatc atccatactt tattaataca
     7441 attttttcac gcaaaagcct gcgattcatt tatgtaatcg caggcttttt tattgctaac
     7501 tttttaattt tatatattta gacatacggt attctattta ttcttttggt ccaacccatt
     7561 caataacatc ttcataagct tggcgtgttt tatttttcgg tggctcttgt tgtctgtagc
     7621 caaatgcgac catcactgat aaaccaaatt gctcagtatc taagatccct ttatttgcta
     7681 aaatgtctgt cactgtatcc agactaaaac cttccatcgg acatgaatca atacctaaca
     7741 atgcggctgt cgtcatcata ttgcctaatg caatatacgt ttgtttactt gaccagtcat
     7801 acaaggcttg atcattatca gaaatatgga aatctgcttg gaatgcatcg aatttttgtt
     7861 caacagctgg aatcgtttgt gcctcatatt ttttaatatc tcttaacata tgttgtacat
     7921 acggtgatct tgacgttaca tttttacgcg caaaaattag cacaaaatgg ctcgctgtat
     7981 ctaattgttt tgctgcgccc caactgtgcg cttttaattc atcacgtaac gcttgatctt
     8041 gaatcacgac aaacttccaa ggttctaacc caagagaact tggagacaat ctacctgact
     8101 ctaatattgt ttcaaaatct tctttcgaaa ctttcttttg tggatcgaat tgcttagtcg
     8161 catgtctgaa atgaaatgca tccataattg tttgattcat attgctcatt gaacatagcc
     8221 tcccattttt atatttattg ctatacaaat tatacttcat gttttaaaaa gactaaagta
     8281 atccgtttgc aattcactat aacttgtgca catgttataa gttttctgca ataaaacaca
     8341 cttttgaatt ttgggaaatt tagtgttata atatatttgt gatatttttc acaattaaat
     8401 tgtggcgtga ttttaatgtg cataatcttt aatttcactc atttactttt caatctttta
     8461 aaagcgaggt ttttaataat gacaaaaatt atgttctttg gtacgcgtga ttatgagaaa
     8521 gagatggcat taaattgggg gaaaaagaat aatgtcgaag taactacttc taaagagcta
     8581 ttatcaagtg ctacagtcga tcaattaaaa gattacgatg gcgtaactac aatgcaattt
     8641 ggtaagttag aaaatgacgt ttatcctaaa ttagaatctt acggtattaa acaaattgca
     8701 caacgtactg ctggatttga tatgtatgat ttagatttag ctaaaaaaca caatattgtg
     8761 atatctaacg ttcctagtta ttcacctgaa acaattgcag agtattctgt ttctatcgcc
     8821 ctacaattag tgcgtcgctt cccagatatt gaacgccgtg tacaagcaca tgattttact
     8881 tggcaagcag aaatcatgtc taaaccagtt aaaaatatga ctgttgcaat tattggtacg
     8941 ggtcgtatcg gtgctgctac agctaaaata tatgcaggat ttggtgctac aattacagct
     9001 tatgacgcct atcctaataa agatttagac tttttaactt ataaagatag tgttaaagaa
     9061 gctattaaag atgccgatat tatttcttta catgttccag cgaacaaaga aagctatcat
     9121 ttattcgata aagcaatgtt tgatcatgtg aaaaaaggtg caatcttagt taacgcagca
     9181 cgtggtgcag tcatcaatac acctgattta atcgctgcag tgaacgatgg tactttgtta
     9241 ggtgctgcga ttgatactta tgaaaatgaa gcagcatact tcacaaatga ctggactaat
     9301 aaagacattg acgataaaac attattagag ttaatcgaac atgaaagaat tttagtaaca
     9361 ccacatattg ctttcttctc tgatgaagca gtacaaaacc ttgttgaagg tggtttaaac
     9421 gcagcattat ctgtcattaa cactggtaca tgtgaaacac gtttaaatta atatatatat
     9481 ttaaaagcaa aggcgcgcca tgtgcctttg ctttttattt tgtatatctg tatcaaaatc
     9541 gatttgatta aaatccgctt tattttatca tctattcaaa tgattttagt gcgcttattt
     9601 tactatggca ttattgccta acctgtttga gatatattaa tatttgatga ttcgtattca
     9661 aagttttcat ttaacatgta tttagtatca tgatagctgc ttcattgatg atatactacc
     9721 tactttttca acacttcttg ttcgataatc gttaatacac cctgcttatc attacttgga
     9781 gcgacagcag aggcaatatt gaacaattct tcatcatgac tattttccat cacataacta
     9841 tgcttagcga acgctaacat atctttatca ttattcgcat ctccgaaggc catgagctct
     9901 gaaggagaca tttcccattt atctaacaat cgttttaatg cctgcccttt agtcatgttt
     9961 ggcataataa tatcaatgct atcgttccca cttgagacaa gtttaatatc attgctgaat
    10021 tgcgttgcta cttcttcgtc aacattcgga tgcgtctcac gattaatatt aaatgctatt
    10081 ttgacataat catcctcagg taattgctgt agactgtcaa tttcttttaa ctgatgataa
    10141 taaaatctcg tatcttcttt aaacgcttca gaagtatgtt ttaaaatata cgcgcttttc
    10201 aaaccacaga tgacgagttg atcaatactt tgcttcatat ttaagtaatc gacaacctgt
    10261 tgaaacacct gacgattaaa gcttttataa ttatataact cattgccatt ataaataact
    10321 gcaccatttt cagaaataaa atacatatct ctatccccga aaatagactt caacttcgca
    10381 tattgattgc cactcgcagc aataaatgta atatctctat ttctaagttc agtaaaaatc
    10441 gcttcaaatc taagtttatc atatgtcttt tttgaatcta aaaacgtccc gtccatatct
    10501 accgcaatag ccttaaccat gattatcctc ctcattgctc ttttagtcgt cttcattata
    10561 tccatctcat gacaagcaat gaagtttgtc ataccacaat gaacctatat tcacattttg
    10621 aaattgcttt catatatgca aaacacatta cactggatat agcattcaga ggcgaacata
    10681 aatttcagaa tattgtaaaa atatcacact atatttatca tttagacaaa atatagtgtg
    10741 atatactaat ttataacatc tatacataat gtgaaagaa
//
LOCUS       DAKTIO010000091        10763 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.091, whole genome shotgun sequence.
ACCESSION   DAKTIO010000091 DAKTIO010000000
VERSION     DAKTIO010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10763
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.091"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(4..1017)
                     /locus_tag="O0293_001715"
     CDS             complement(4..1017)
                     /locus_tag="O0293_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCY0280600.1"
                     /translation="MAIKYSALNLVPIREGEDEQTAINDMVNLAQHLDELSYERYWIA
                     EHHNAPNLVSSATALLIQHTLEHTKHIRVGSGGIMLPNHAPLIVAEQFGTMATLFPNR
                     VDLGLGRAPGTDMMTASALRRDQHDGVYKFPEEVSLLQQYFGPAHQQAYVRAYPAVGK
                     NVPLYILGSSTDSAHLAARKGLPYVFAGHFAPQQMKEAIEIYKTLFEPSDVLDEPYVI
                     VCLNTIVAENDDEAQYLASSMAQVMVSITRGRMQPVQPPTHELQNILTPREYAMAMER
                     QKISLIGSENTVQQKIQDFMETYGEVNEIMAISYIYDKDMQLDSYRRFKNVINQINEK
                     NTL"
     gene            1329..2471
                     /locus_tag="O0293_001716"
     CDS             1329..2471
                     /locus_tag="O0293_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCY0280601.1"
                     /translation="MEIYADYAATTPVKPEVVDAMMTIYNSHYGNPSSIHAKGRDARK
                     YLDESRRQIAQLLGADTHEIIFTSGATESNNTAIKGIVKANEQLGNHIITSKIEHHSV
                     LHVFEQLEREGFDVTYLDVDDTGAIDLDQLEETITDKTILVSIMFVNNEVGTVQQIYD
                     IQDIIAETNAYFHVDAVQAIGHLDVKFDEFEIDAMSITAHKFGGPKGVGALLVKDHVT
                     LDYPQLGGEQELKRRAGTENLAQIVGMAKALQLAEKNRDDNNIHLMNLKEQFLVKLQE
                     RAIPFELNGSMTDATGHIVNLYFPFVEVETMLTLLDMAQIYVSSGSACTAGSTQPSHV
                     LDAMFEDEERSNHSIRFSFNELTTENEINAIVAEIHKIYFKFKEES"
     gene            2472..3590
                     /gene="mnmA"
                     /locus_tag="O0293_001717"
     CDS             2472..3590
                     /gene="mnmA"
                     /locus_tag="O0293_001717"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCY0280602.1"
                     /translation="MSNKDIRVVVGMSGGVDSSVTAHVLKEQGYDVIGIFMKNWDDTD
                     ENGVCTATEDYNDVIEVCNQIGIPYYAVNFEKEYWDKVFTYFLDEYKKGRTPNPDVMC
                     NKEIKFKAFLDHAMNLGADYVATGHYARIHRHEDGHVEMLRGVDNNKDQTYFLNQLSQ
                     QQLSKVMFPIGDIEKSEVRRIAEEQGLVTAKKKDSTGICFIGEKNFKTFLSQYLPAQP
                     GDMITLDGKKMGKHSGLMYYTIGQRHGLGIGGDGDPWFVVGKNLKDNVLYVEQGFHHD
                     ALYSDYLIASDYSFVNPEDNDLDQGFECTAKFRYRQKDTKVFVKRENDHALRVTFAEP
                     VRAITPGQAVVFYQGDVCLGGATIDDVFKNEGQLNYVV"
     gene            4052..4720
                     /locus_tag="O0293_001718"
     CDS             4052..4720
                     /locus_tag="O0293_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCY0280603.1"
                     /translation="MIDQQTIYQYIQNGKIEEALQALFGNIEENPTIIENYINAGIVL
                     ADANEIEKAERFFQKALTIDPKNGVVFFNLANVYYNQQRYQEAIKLYQQALQTEIEQV
                     DCNYMIGMAFNQLESFKLALPYLMTAAELDKDKDAEVQFQYGLVLCQLEMFNEAITQL
                     KHVLTIDKNHVDARYNLGLALFMKNEDIDEAITHFKEAVTIDPKHLLSQHALKTFTKM
                     KEEE"
     gene            4722..7199
                     /locus_tag="O0293_001719"
     CDS             4722..7199
                     /locus_tag="O0293_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HCY0280604.1"
                     /translation="MSDPTLFDYSMIKGTVEAILFQNSDNFYTVLKVDTIETNEDFDT
                     MPTVVGFLPNIVEGDVYTFKGQVVDHPRYGKQLKAETFEKEMPQTKEAIISYLSSDLF
                     KGVGKKTAQNIVNTLGDNAINDILDDHSVLEKVSGLSKKKQKQIAEQISANQESEKIM
                     IRLHDLGFGPKLSMAIYQFYLGDTLTILDRNPYQLIYDIKGIGFNKADQLARNIGIAY
                     NDNERLKAALLYTLEEECIKQGHTYLPINVVIDLTVDVLNYQDEEVIEPEKLDEMLQY
                     LNEEKRLIIDNEQVAIPSLYYSEIKSVQNLFRIKTHTNKLTEIEQSDLQMHIGEIEDA
                     NQVNYAASQKEALQTAINSKVMLLTGGPGTGKTTVIKGIVELYAEIHGLSLDYDDYVN
                     DDYPVVLAAPTGRASKRLQESTGLEAMTIHRLIGWNQDTKPEDILENEINARLIIIDE
                     MSMVDTWLFHQFLSAVPLDAQLIFVGDEDQLPSVGPGQVFKDLIESKAIPRVNLTEVY
                     RQQDGSSIIELAHRMKLGQKIDITQRFHDRSFINCQANQIPTVVEKVVTSAVNKGYTM
                     ADIQVLAPMYKGNAGIKRLNQVLQDILNPKKKDTREIEFGDVVFRKGDKVLQLVNRPN
                     DNIFNGDIGVIVGIFWAKENALNKDVLVVDFEGNEITFTKQDMMELTHAYCTSIHKSQ
                     GSEFPIVIMPIVKQYFRMLQRPILYTGLTRAKTSLVLLGDPEAFDIGLKTNGQARLTQ
                     LCTLLKNYFNSEDEEMLENTATNDTGASQTTIDDQVEAPMSSNDSEEVMSDSTKTDTN
                     VLTEATMFKIDPMINMGEITPYDFIER"
     gene            7542..10172
                     /gene="alaS"
                     /locus_tag="O0293_001720"
     CDS             7542..10172
                     /gene="alaS"
                     /locus_tag="O0293_001720"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCY0280605.1"
                     /translation="MKKLKASEIRQKYLDFFVEKGHMVEPSAPLVPIDDDTLLWINSG
                     VATLKKYFDGRETPKKPRIVNSQKAIRTNDIENVGFTARHHTFFEMLGNFSIGDYFKQ
                     EAIEFAWEFLTSDKWMGMEPDKLYVTIHPEDMEAYNIWHKDIGLEESRIIRIEGNFWD
                     IGEGPSGPNTEIFYDRGEAYGQDDPAEEMYPGGENERYLEVWNLVFSEFNHNKDHSYT
                     PLPNKNIDTGMGLERMASVSQNVRTNYETDLFMPIMNEIEKVSGKQYLVNNEQDVAFK
                     VIADHIRTIAFAISDGALPANEGRGYVLRRLLRRAVRFSQTLGINEPFMYKLVDIVAD
                     IMEPYYPNVKEKADFIKRVIKSEEERFHETLEDGLAILNELIKKAKATTNEINGKDAF
                     KLYDTYGFPIELTEEIAVQAGLKVDMTTFESEMQQQRDRARQARQNSQSMQVQSEVLK
                     NITSASTFVGYDTATAQTTLTHLIYNGEEVSQVEAGETVYFMLTETPFYAISGGQVAD
                     TGIVYNDNFEIAVSEVTKAPNGQNLHKGVVQFGQVNVGATVSAEVNQNDRRDIQKNHS
                     ATHLLHAALKSVLGDHVNQAGSLVEADRLRFDFSHFGPMTNDEIDQVERLVNEEIWKG
                     IDVNIQEMDIASAKEMGAMALFGEKYGDVVRVVNMAPFSIELCGGIHVRNTSEIGLFK
                     IVSESGTGAGVRRIEALTGKAAFLYLEDIQEKFNTMKSQLKVKSDDQVVDKLTQLQDE
                     EKALLKQLEQRDKEITSLKMGNIEDQVEEINGYKVLVTEVDVPNAKAIRSTMDDFKSK
                     LQDTIIILASNVDDKVSMVATVPKSLTNNVKAGDLIKQMAPIVGGKGGGRPDMAQGGG
                     TQPENISKSLSFIKDYIKNL"
     gene            10235..10495
                     /locus_tag="O0293_001721"
     CDS             10235..10495
                     /locus_tag="O0293_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HCY0280606.1"
                     /translation="MENFDKTMKFDYEELPTQDVRDVLNNVYRTLDERGYNAVNQIVG
                     YLLSGDPAYIPRQNEARNQIRHIDRDVIMEELVSYYLKEQNK"
     gene            10499..>10763
                     /gene="ruvX"
                     /locus_tag="O0293_001722"
     CDS             10499..>10763
                     /gene="ruvX"
                     /locus_tag="O0293_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCY0280607.1"
                     /translation="MLQHKILGLDVGSRTVGIAISDIMGWTAQGLDTLRINEENNELG
                     IDQLVDIIKKHNVGTVVIGLPKNMNNSIGFRGEASLTYKEKLLE"
BASE COUNT         3880 a         1521 c         2089 g         3273 t
ORIGIN      
        1 acattataaa gtgttttttt catttatctg atttataaca ttcttgaacc gacgataaga
       61 gtctaattgc atatctttat cataaatata acttattgcc ataatttcgt tgacttcacc
      121 ataagtttcc ataaaatctt gaattttttg ttgaacagta ttttctgaac ctattaatga
      181 tattttctgt ctttccatag ccatcgcgta ttctctcggc gttaatatat tttgtagttc
      241 atgtgttggc ggttgaacgg gctgcattct gccacgagtg atactaacca ttacttgtgc
      301 catagatgaa gctaaatatt gtgcttcgtc atcattttca gcaacgattg tatttaaaca
      361 tacaataaca taaggttcgt ctaatacatc agaaggttca aataacgttt tgtaaatttc
      421 gatagcttct ttcatttgtt gaggtgcaaa atgtccagcg aacacatatg gaagcccttt
      481 gcgagcagct aaatgtgcag aatctgttga agaaccaaga atgtataaag gcacattttt
      541 acctactgct ggataagcac gaacatatgc ttgttggtga gcagggccga aatattgttg
      601 taataatgaa acctcttctg gaaatttata aacaccatca tgttgatctc gtcttaatgc
      661 acttgcggtc atcatatctg ttccaggtgc acgtcctaat cctaaatcga cacgatttgg
      721 aaataatgtt gccatcgtgc caaattgttc cgcaacgatt aatggagcat gattaggtaa
      781 catgatgcct ccagaaccta cacgtatgtg tttcgtatgt tctaacgtat gttgaattaa
      841 taaagcagtt gctgaactta ctaggttggg agcgttatgg tgttcagcaa tccaatatct
      901 ttcatatgat aattcgtcta aatgttgtgc gagattaacc atatcattaa ttgctgtttg
      961 ttcatcttca ccttctcgaa tagggacaag gtttaatgct gaatatttaa tcgccatttt
     1021 tatatcgtcc ttcctttttt atgatataag aagtatacca atcgaataac cagataaata
     1081 ttaaattgct cataaccaaa ataatagtac gttacatttt acaaataaaa aaatatctgc
     1141 aaatactaaa atttataaaa aagacaagaa taaagcgata aacttaaaaa agcctatcga
     1201 aaagcatgtg tagaaaatgg taaaatataa ttaaaaacat tataaataaa gttaaacaga
     1261 aaagtagaaa aatagtatgt aaggtgctat tattggaaca ttgataaaat tgtgaggagt
     1321 gtccaaacat ggaaatttat gcagattatg ctgcgaccac accagtaaaa cctgaagtag
     1381 tagatgcgat gatgacgatt tacaattcgc attatggtaa tccatcatca atacatgcta
     1441 agggaagaga tgcgcgtaaa tatttagatg agtcaagacg tcaaattgca caattacttg
     1501 gtgcagatac acatgaaatt atatttacga gtggcgcaac agaatcgaat aatacggcaa
     1561 ttaaaggcat agtaaaagca aatgaacaat tgggtaatca cattatcaca tcgaagattg
     1621 aacatcattc tgtgttacat gtttttgaac aattagagcg agaaggtttt gatgtaacat
     1681 atctagatgt tgatgataca ggtgctattg atttagatca acttgaagaa acaattacag
     1741 ataagacgat tttagtatct attatgtttg taaataatga agttggcaca gtgcaacaaa
     1801 tttatgatat tcaagatatt attgctgaga caaatgcata tttccatgtt gatgcggttc
     1861 aagcaattgg acatcttgat gttaagtttg acgaatttga gatcgacgca atgagtatta
     1921 ctgctcataa gtttggcgga ccaaaaggtg tcggtgcatt actagttaaa gaccatgtta
     1981 cactagatta tcctcaactt ggcggtgaac aagaattgaa acgccgtgca ggcactgaaa
     2041 atcttgcgca aattgttggg atggctaaag cattacaatt agcagaaaag aatcgagacg
     2101 ataacaatat tcatttaatg aatttaaaag aacaattttt agttaaatta caagaaagag
     2161 cgattccatt tgaattaaat ggttcaatga cggatgctac aggccatatt gtaaatctat
     2221 attttccgtt tgttgaagtt gaaacgatgt taacattgtt ggatatggca caaatctatg
     2281 tgtcgtctgg atctgcatgt acagcaggct ctacacaacc gtcgcatgtt ttagatgcaa
     2341 tgttcgaaga tgaggagcga tcaaatcatt cgattcgatt tagttttaac gaattgacta
     2401 ctgaaaatga aattaatgca attgtagctg aaattcataa aatatatttt aaatttaagg
     2461 aggagtcata attgtcaaat aaagatatac gtgttgtcgt tggtatgtca ggcggtgtag
     2521 atagttctgt aacagcccac gtcttaaaag aacaaggtta tgatgtcatt ggcatattta
     2581 tgaaaaactg ggatgacact gacgaaaatg gcgtatgtac tgcaactgaa gattacaacg
     2641 atgttattga agtgtgtaat caaattggca ttccgtatta cgctgttaat tttgaaaaag
     2701 aatattggga taaagtcttt acgtatttct tagatgaata caaaaaaggt cgtactccaa
     2761 atccagacgt tatgtgtaat aaagaaatta agtttaaagc ctttttagat catgcgatga
     2821 atttaggtgc agattatgta gcaacaggac attacgcacg catacatcgt catgaagatg
     2881 gtcatgttga aatgttacgt ggtgtagata ataataaaga tcaaacatac ttcttgaatc
     2941 aattatctca acaacaactt tcaaaagtga tgttcccaat tggcgacatc gaaaagagtg
     3001 aagtgcgtcg aattgctgaa gaacaaggac ttgttactgc taagaaaaaa gattctacag
     3061 gcatttgttt tatcggcgaa aaaaacttta aaacattttt atcacaatat ttacctgcac
     3121 aaccgggtga tatgataaca cttgatggta agaaaatggg taaacatagt ggtttgatgt
     3181 attacacaat aggacaaaga catggattag gtataggtgg agatggcgat ccttggtttg
     3241 ttgtcggtaa aaacctaaaa gataatgttt tatatgtaga acaaggattc catcacgatg
     3301 cattatacag tgattactta attgcttcag actattcatt tgtaaatcca gaagataatg
     3361 acttagatca aggttttgaa tgtacagcta aatttagata tcgccaaaaa gatacgaaag
     3421 tttttgtgaa acgtgaaaac gaccatgcac tacgtgttac ttttgctgag ccagtaagag
     3481 caatcacacc tggacaagca gttgtttttt atcaaggtga tgtgtgtctt ggtggtgcaa
     3541 caattgacga tgtgttcaaa aatgaaggtc aattaaatta tgttgtataa acaatggcaa
     3601 caataaatta cttatttgaa gtttcaacgt tgaaaatgac gaaagacagt ttttgatgag
     3661 aataattcat gaggatagag tctgggacat cacaatgtcc taggctctac aatgttatat
     3721 tggcgggacc acaacataga gaatttcgta aagaaattct acaggcaatg ccagttgggg
     3781 ataacgaatt taattttgtt aaaatatcat ttctgtccca ctccctatgc atgaatctaa
     3841 ttatgtattc ttatttttaa gtacataata gtggtggcta atgtggaaga accattacat
     3901 aataaaccgt taatggttct taagcatttc tattccattc ccgctttttc atgaatgaag
     3961 atgatattag attatatttt attcgttgtt aagtgattcg agacatacaa tttatcaaga
     4021 tgttataatt gatgagaaat gaggttcgta aatgatagat caacaaacaa tttatcaata
     4081 catacaaaat ggaaaaatag aagaagcgtt acaagcattg ttcggaaata tcgaagaaaa
     4141 tcctacaatt attgaaaatt atattaatgc tggtatcgta cttgctgatg cgaatgagat
     4201 tgaaaaggca gagcgttttt tccaaaaagc tttaacaata gatccgaaga atggcgtcgt
     4261 attttttaat ctagcaaatg tatattataa tcagcaacgt tatcaagaag ctattaaatt
     4321 atatcaacaa gcattacaaa cagagattga acaagttgat tgtaattata tgatcggtat
     4381 ggcgtttaat cagttagaat catttaagct ggcattgccg tatttaatga ctgctgcgga
     4441 actagataaa gacaaagatg cagaagttca atttcaatat ggtcttgtat tatgtcaatt
     4501 agaaatgttt aatgaagcca taactcaact taaacatgta ttaacgattg ataaaaatca
     4561 tgttgatgca agatacaatt tgggcttagc gttatttatg aaaaatgaag atattgatga
     4621 agcaataact cattttaaag aagctgtgac tatcgaccct aaacacttat taagtcagca
     4681 tgcgctgaaa acattcacta aaatgaaaga ggaggagtaa catgtcagac cctacactat
     4741 ttgattattc aatgatcaaa ggtactgttg aagcgatatt gtttcaaaac agtgataatt
     4801 tttatacagt gcttaaagta gatactattg aaacaaatga agactttgat acaatgccaa
     4861 ctgttgtagg gtttcttccg aatattgttg aaggcgatgt ctacacattt aaagggcaag
     4921 ttgttgatca tccacgttat ggtaaacaat taaaagcaga gacttttgaa aaagaaatgc
     4981 cacaaacgaa agaagcaatt ataagttatt tatctagtga tttgtttaaa ggtgtaggta
     5041 aaaagacagc tcaaaatatt gttaatacat taggtgacaa tgcgataaat gatatattag
     5101 atgaccattc agtgctagaa aaagtttcgg gattatctaa aaagaaacag aaacaaattg
     5161 cagaacaaat ttcagcaaat caagaatctg aaaaaataat gattcgttta catgatttag
     5221 ggtttggtcc taaactttca atggcaattt atcagtttta tctcggcgat acactgacta
     5281 ttttagatcg aaatccatat caattgattt atgatattaa aggtattggt tttaataaag
     5341 cagatcaact tgctagaaat attggtatcg cttataacga taatgaacga ttaaaagctg
     5401 cgttgctcta tacgttagaa gaagaatgta ttaaacaggg acatacatat ttgcctatca
     5461 atgttgttat agatttaacg gttgatgtat taaattatca agatgaagag gtcatcgaac
     5521 ctgaaaaatt agacgaaatg ttgcaatact taaatgagga aaaacgacta attattgaca
     5581 atgaacaagt tgcaattcca agtttgtatt attctgaaat caaaagtgtt caaaacttat
     5641 tcagaataaa gacgcataca aataaactta cagaaattga acagtcagat ttacaaatgc
     5701 atattggtga aatagaagat gcgaatcaag tgaactatgc agcttcacaa aaagaagctt
     5761 tgcaaacggc tatcaattct aaggtgatgc tattaaccgg tggaccggga acaggaaaaa
     5821 cgacagtaat taaaggtatt gttgaattat atgctgagat tcatggctta tcattagatt
     5881 atgatgatta tgtcaatgat gattatccag ttgtattagc agcgccaaca ggaagagcgt
     5941 ctaagagact acaagagtca actggattag aagcaatgac gattcatcgt ttaataggtt
     6001 ggaatcaaga cacaaagcca gaggatatat tagaaaatga aataaacgca cgactcataa
     6061 tcattgatga gatgtctatg gtagatactt ggttgttcca ccaattttta agtgcagttc
     6121 ctttagatgc acaattgata tttgtaggtg atgaggacca attgccttct gtaggtcctg
     6181 gtcaagtatt taaagattta attgaatcga aagctatacc acgagtaaat ctgactgaag
     6241 tatatcgtca acaagatggt tcaagcatta tagaattagc acatcgaatg aagttgggtc
     6301 agaaaattga tattacacag cgttttcatg atagaagttt tattaactgt caggcaaacc
     6361 aaataccaac agttgttgag aaggtagtta ctagtgctgt taataaaggg tatactatgg
     6421 ctgatattca agtgcttgca ccaatgtata aaggaaatgc cggtattaaa cgtttaaatc
     6481 aagttttaca agatatttta aaccctaaaa agaaagacac acgagaaatt gagtttggtg
     6541 atgtagtctt tagaaaaggt gacaaagtat tacaacttgt taataggcca aatgataaca
     6601 tattcaatgg tgacatagga gttattgttg gtattttttg ggcgaaagaa aatgccctta
     6661 ataaagatgt acttgttgtg gattttgaag gtaatgaaat tacatttacg aaacaagata
     6721 tgatggaatt aacacatgct tattgtacat caatccataa atcacaaggt tcagaatttc
     6781 ctattgttat catgcctatt gttaaacagt attttagaat gttacaaaga ccgatattat
     6841 atactggttt aactagagcg aaaacgagtt tagttttact tggtgatccg gaagcatttg
     6901 atattggatt gaaaacaaat ggtcaagcgc gtttaacaca attatgtaca ttgttgaaaa
     6961 attattttaa ttcagaagat gaagaaatgc tggaaaacac agcaactaat gatacaggag
     7021 cttcacaaac gacaatagat gatcaagtag aagcgccaat gtcatcgaat gatagtgaag
     7081 aagttatgtc tgattcaaca aaaacagata caaatgtatt aacggaagct acaatgttta
     7141 aaattgaccc aatgattaat atgggagaaa tcacgccgta tgactttata gaacgttgac
     7201 aacaagcgta aatgtaacta aaatagaatt aaattcgtat aaagatgagt agttaatgat
     7261 ttgtttatca gagatgtact ggttggtgaa aagtacaaca aacattagtg aacgctactt
     7321 tatgcacaac tgtatagtcg aattaaagtg ataaatgtgt tgaattgcat ttataactag
     7381 ggtggtaccg cgataacgtt cgtccctttt gaggatgggc gttttttatt ttttaataat
     7441 aaaaaatgat attgatttat actagttttg caaatcgact atctgtgtca tcaattttta
     7501 tggaatggta atttaaaaac agtaaatagg atgtgtaaat gatgaaaaag ttaaaagcga
     7561 gtgaaattag acaaaaatat ctagatttct ttgttgaaaa aggacatatg gttgaacctt
     7621 ctgcaccatt agtgccaatt gatgatgata cattattatg gattaattca ggtgtagcaa
     7681 cattaaagaa atattttgat ggacgtgaaa cacctaaaaa gccaagaatt gtaaactctc
     7741 aaaaagctat tcgtacaaat gatattgaaa atgttggttt cacagcgcgt caccatacat
     7801 tctttgaaat gttaggtaac ttctcaattg gtgattattt taaacaagaa gcgattgaat
     7861 ttgcttggga atttttaacg agtgataaat ggatgggtat ggagccagat aaattgtacg
     7921 ttacgattca tccggaagat atggaagcat acaacatttg gcataaagat attgggcttg
     7981 aagaaagtcg tattattcgc attgaaggta acttctggga tattggtgaa gggccttcag
     8041 gaccgaacac tgagattttc tatgatcgcg gagaagcata tggacaagac gatccggcag
     8101 aagaaatgta tccaggtgga gaaaatgaac gctatcttga agtatggaac ttagtattta
     8161 gtgaattcaa tcataataaa gatcatagtt acacaccatt acctaataaa aatattgata
     8221 ctggcatggg gcttgagcgt atggcctcag tttctcaaaa tgtacgtact aactatgaaa
     8281 cagatttatt tatgcctata atgaatgaaa tcgaaaaagt atcaggtaaa caatatttag
     8341 taaacaacga acaagatgtg gcatttaaag taattgctga ccacattcgt acgattgcat
     8401 ttgcaatttc tgatggtgca ttacctgcca atgaaggtag agggtatgta ttacgtcgat
     8461 tgttacgtcg tgccgttcgt tttagtcaaa cgttaggaat caatgagcca tttatgtaca
     8521 aacttgttga tattgttgca gacattatgg aaccatatta tccaaatgtt aaggaaaaag
     8581 cagatttcat taagcgtgtt ataaagtctg aagaagaacg attccatgaa acattagaag
     8641 atggtttagc gattttaaat gaattaatta aaaaagctaa agcgacaaca aatgaaatta
     8701 atgggaaaga tgcatttaaa ttgtatgata cgtatgggtt cccaattgaa ttaactgaag
     8761 aaatagcagt gcaagcagga ttgaaagttg atatgacaac attcgagtca gaaatgcaac
     8821 aacaacgtga tcgtgcacgt caagcacgtc aaaattctca atcaatgcaa gttcaaagtg
     8881 aagtattgaa aaatattaca tctgcaagta cttttgttgg ttatgatact gcgacagctc
     8941 aaacaacact aacacacttg atatacaatg gtgaagaagt ttcacaagtt gaagcgggtg
     9001 aaacagtata cttcatgtta acggaaacac cattttatgc aatcagtggt ggacaagttg
     9061 cggatacagg tattgtttat aatgacaatt ttgaaattgc tgttagtgaa gtaaccaaag
     9121 caccaaatgg tcaaaacttg cataaaggag tagtacaatt tggccaagta aatgttggcg
     9181 ctacagtgtc tgctgaagtg aaccaaaatg atcgacgtga cattcaaaag aaccatagtg
     9241 caacacattt attacatgca gcgttgaaat cagtactggg tgatcatgtt aaccaagctg
     9301 gttcactagt agaagcagat cgtttacgtt ttgatttctc tcattttggt ccaatgacta
     9361 atgatgaaat tgatcaagtt gaacgcttag taaatgaaga aatttggaaa ggtattgacg
     9421 ttaacattca agaaatggat attgcttcag ctaaagaaat gggcgcaatg gcattattcg
     9481 gtgaaaaata tggtgatgtt gtgcgtgtag taaatatggc accattttca attgaattat
     9541 gtggtggtat tcatgtccgc aatacttctg aaattggctt attcaaaata gtaagtgagt
     9601 caggtacagg agctggtgtg cgtcgtattg aagcattaac aggtaaagca gctttcttat
     9661 atttagaaga tattcaagag aaatttaata cgatgaaatc acagctgaaa gtgaaatctg
     9721 atgatcaagt agtcgataag ttaacacaat tacaagatga agaaaaagca ttattaaaac
     9781 aattagagca acgtgacaaa gaaatcacat cacttaaaat gggtaatatt gaagatcaag
     9841 ttgaagaaat caatggctat aaagtattgg ttactgaagt ggatgtacca aatgcgaaag
     9901 caattcgctc gacaatggac gattttaaat ctaaactaca agatacaatt atcattcttg
     9961 caagtaatgt tgatgataaa gtatcgatgg ttgcaactgt ccctaaatct ttaacaaata
    10021 acgttaaagc cggtgatctt atcaaacaaa tggcaccaat cgttggtggt aaaggtggcg
    10081 gtcgtccaga tatggctcaa ggtggcggta cacaacctga aaatatctca aaatcattaa
    10141 gctttattaa agattacatt aaaaatctat aatttaaagc cataatattg tatgatgata
    10201 gtgagttata tttgtaatta aaggagtgtc gcaaatggaa aactttgata aaacaatgaa
    10261 attcgactat gaagaacttc caacgcaaga tgtaagagat gttttaaata atgtttatcg
    10321 aacattagat gaacgaggat ataatgccgt aaaccaaatt gtaggttatt tattatcagg
    10381 tgaccctgcg tatattccac gccaaaatga agcaagaaat caaattcgtc atattgatag
    10441 agatgttatt atggaagagc ttgtttctta ctatttaaaa gagcaaaata aataatttat
    10501 gttacaacat aaaattttag gactcgatgt cggtagtaga acggtaggaa ttgcaattag
    10561 tgatataatg ggttggacgg cacaaggatt agacacactc cgaatcaatg aagaaaataa
    10621 tgaattaggt attgaccaat tagtagacat tattaaaaaa cataatgtgg gtacagtcgt
    10681 aataggacta cctaaaaaca tgaataattc aataggattt cgaggcgaag cttcgttaac
    10741 atataaagaa aaattattag aag
//
LOCUS       DAKTIO010000092        10695 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.092, whole genome shotgun sequence.
ACCESSION   DAKTIO010000092 DAKTIO010000000
VERSION     DAKTIO010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10695
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.092"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..594)
                     /locus_tag="O0293_001723"
     CDS             complement(<1..594)
                     /locus_tag="O0293_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein"
                     /protein_id="HCY0280608.1"
                     /translation="MKFTVIAKAIFILGILTTSVMITENQSVNAKGKYEKMNRLYDTN
                     KLHQYYSGPSYELTNVSGQSQGYYDSNVLLFNQQNQKFQVFLLGKDENKYKEKTHGLD
                     VFAVPELVDLDGRIFSVSGVTKKNVKSIFESLRTPNLLVKKIDDKDGFSIDEFFFIQK
                     EEVSLKELDFKIRKLLIKKYKLYEGSADKGRIVINMKD"
     gene            complement(938..1633)
                     /locus_tag="O0293_001724"
     CDS             complement(938..1633)
                     /locus_tag="O0293_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL7"
                     /protein_id="HCY0280609.1"
                     /translation="MKLKTLAKATLALGLLTTGVITSEGQAVQAKEKQERVQHLYDIK
                     DLHRYYSSESFEFSNISGKVENYNGSNVVRFNQENQNHQLFLLGKDKEKYKEGIEGKD
                     VFVVKELIDPNGRLSTVGGVTKKNNKSSETNTHLFVNKVYGGNLDASIDSFSINKEEV
                     SLKELDFKIRQHLVKNYGLYKGTTKYGKITINLKDGEKQEIDLGDKLQFERMGDVLNS
                     KDINKIEVTLKQI"
     gene            complement(2054..2749)
                     /locus_tag="O0293_001725"
     CDS             complement(2054..2749)
                     /locus_tag="O0293_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL6"
                     /protein_id="HCY0280610.1"
                     /translation="MKLKTLAKATLVLGLLATGVITTESQTVKAAESTQGQHNYKSLK
                     YYYSKPSIELKNLDGLYRQKVTDKGVYVWKDRKDYFVGLLGKDIEKYPQGEHDKQDAF
                     LVIEEETVNGRQYSIGGLSKTNSKEFSKEVDVKVTRKIDESSEKSKDSKFKITKEEIS
                     LKELDFKLRKKLMEEEKLYGAVNNRKGKIVVKMEDDKFYTFELTKKLQPHRMGDTIDG
                     TKIKEINVELEYK"
     gene            complement(3195..3899)
                     /locus_tag="O0293_001726"
     CDS             complement(3195..3899)
                     /locus_tag="O0293_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL5"
                     /protein_id="HCY0280611.1"
                     /translation="MKMTAIAKASLALGILATGTITSLHQTVNASEHKAKYENVTKDI
                     FDLRDYYSGASKELKNVTGYRYSKGGKHYLIFDKNRKFTRVQIFGKDIERFKARKNPG
                     LDIFVVKEAENRNGTVFSYGGVTKKNQDAYYDYINAPRFQIKRDEGDGIATYGRVHYI
                     YKEEISLKELDFKLRQYLIQNFDLYKKFPKDSKIKVIMKDGGYYTFELNKKLQTNRMS
                     DVIDGRNIEKIEANIR"
     gene            complement(4263..5189)
                     /locus_tag="O0293_001727"
     CDS             complement(4263..5189)
                     /locus_tag="O0293_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL4"
                     /protein_id="HCY0280612.1"
                     /translation="MKITTIAKTSLALGLLTTGVITTTTQAANATTPSSTKVEAPQST
                     PPSTKIEAPQSKPNATTPPSTKVEAPQQTANATTPPSTKVTTPPSTNTPQPMQSTKSD
                     TPQSPTTKQVPTEINPKFKDLRAYYTKPSLEFKNEIGIILKKWTTIRFMNVVPDYFIY
                     KIALVGKDDKKYGEGVHRNVDVFVVLEENNYNLEKYSVGGITKSNSKKVDHKAGVRIT
                     KEDNKGTISHDVSEFKITKEQISLKELDFKLRKQLIEKNNLYGNVGSGKIVIKMKNGG
                     KYTFELHKKLQENRMADVIDGTNIDNIEVNIK"
     gene            complement(5298..5435)
                     /locus_tag="O0293_001728"
     CDS             complement(5298..5435)
                     /locus_tag="O0293_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280613.1"
                     /translation="MFVKVAFLCLKSDETSNVPSVESHQNHFYLTNIMDFLIYLTMIQ
                     I"
     gene            complement(5554..6612)
                     /locus_tag="O0293_001729"
     CDS             complement(5554..6612)
                     /locus_tag="O0293_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL3"
                     /protein_id="HCY0280614.1"
                     /translation="MKMRTIAKTSLALGLLTTGAITVTTQSVKAEKIQSTKVDKVPTL
                     KAERLAMINITAGANSATTQAANTRQERTPKLEKAPNTNEEKTSASKIEKISQPKQEE
                     QKTLNISATPAPKQEQSQTTTESTTPKTKVTTPPSTNTPQPMQSTKSDTPQSPTIKQA
                     QTDMTPKYEDLRAYYTKPSFEFEKQFGFMLKPWTTVRFMNVIPNRFIYKIALVGKDEK
                     KYKDGPYDNIDVFIVLEDNKYQLKKYSVGGITKTNSKKVNHKVELSITKKDNQGMISR
                     DVSEYMITKEEISLKELDFKLRKQLIEKHNLYGNMGSGTIVIKMKNGGKYTFELHKKL
                     QEHRMAGTNIDNIEVNIK"
     gene            complement(6903..7598)
                     /locus_tag="O0293_001730"
     CDS             complement(6903..7598)
                     /locus_tag="O0293_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL2"
                     /protein_id="HCY0280615.1"
                     /translation="MKMKNIAKISLLLGILATGVNTTTEKPVHAEKKPIVISENSKKL
                     KAYYNQPSIEYKNVTGYISFIQPSIKFMNIIDGNSVNNIALIGKDKQHYHTGVHRNLN
                     IFYVNEDKRFEGAKYSIGGITSANDKAVDLIAEARVIKEDHTGEYDYDFFPFKIDKEA
                     MSLKEIDFKLRKYLIDNYGLYGEMSTGKITVKKKYYGKYTFELDKKLQEDRMSDVINV
                     TDIDRIEIKVIKA"
     gene            complement(7884..8564)
                     /locus_tag="O0293_001731"
     CDS             complement(7884..8564)
                     /locus_tag="O0293_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL1"
                     /protein_id="HCY0280616.1"
                     /translation="MKFKAIAKASLALGMLATGVITSNVQSVQAKAEVKQQSESELKH
                     YYNKPILERKNVTGFKYTDEGKHYLEVTVGQQHSRITLLGSDKDKFKDGENSNIDVFI
                     LREGDSRQATNYSIGGVTKSNSVQYIDYINTPILEIKKDNEDVLKDFYYISKEDISLK
                     ELDYRLRERAIKQHGLYSNGLKQGQITITMNDGTTHTIDLSQKLEKERMGESIDGTKI
                     NKILVEMK"
     gene            9036..9881
                     /locus_tag="O0293_001732"
     CDS             9036..9881
                     /locus_tag="O0293_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCY0280617.1"
                     /translation="MNIMLTGATGHLGTHITNQAIANHIDHFHIGVRNVEKVPEDWRG
                     KVPVRQLDYFNQESMVEAFKGMDTVVFIPSIIHPSFKRIPEVENLVYAAKQSGVAHII
                     FIGYYADQHNNPFHMSPYFGYAARLLATSGIDYTYVRMAMYMDPLKPYLPELMNMHKL
                     IYPAGDGRINYITRNDIARGVIAIIKNPDTWGKRYLLSGYSYDMKELAAILSEASGTE
                     IKYEPVSLETFAEMYDEPKGFGALLASMYDAGARGLLDQESNDFQQLVNDQPQTLQSF
                     LQENI"
     gene            9900..10259
                     /locus_tag="O0293_001733"
     CDS             9900..10259
                     /locus_tag="O0293_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HCY0280618.1"
                     /translation="MNIISTILIIFVALEFFYIMYLETIATTSKKTSETFNISVDKLK
                     DKNINLLLKNQGVYNGLIGVLLIYGLFISSNPKEICAAILVYIIGVAIYGGLSSNISI
                     FFKQGTLPVLALISMLW"
BASE COUNT         3159 a         1844 c         1494 g         4198 t
ORIGIN      
        1 atctttcata ttaataacaa ttctaccttt atcagctgac ccttcataca gtttgtattt
       61 tttaatcaac agttttctta ttttaaaatc aagttccttc aatgacactt cttccttttg
      121 aataaagaaa aattcatcaa tagaaaaacc gtctttatcg tctatttttt taactagtaa
      181 gttcggcgtt cttagagact caaatattga ttttacgttt ttctttgtta caccactaac
      241 actaaatatt cttccatcta aatctactaa ttctggtacc gcaaagacat ctaaaccatg
      301 tgttttttct ttgtatttat tttcatcttt tcccaataaa aacacttgga acttttgatt
      361 ttgttggtta aaaagcaaaa cgttagagtc ataataacct tgactttggc cactaacatt
      421 tgttaactca taactaggtc ctgaatagta ttgatgtaac ttgtttgtat catataaacg
      481 gttcattttt tcatactttc cttttgcatt aaccgattga ttttctgtta tcattacact
      541 tgttgttaat attcctaata taaatatcgc tttagctatc actgtaaatt tcatattcat
      601 gtgctccaat cttaatatat tggattgctt ttattacgta atttgaataa tacaccttta
      661 ttataaaggg cattgagaca gactgacatt aacactcgtt taattaaact taaatcattg
      721 ttaatgaaga gctaattaat attcaatggg catcactttc aagagcaatg taaattttta
      781 gtatgctcac tcataacctg cgttataaaa cgtaaaaaaa agcctaacga caaggttgtt
      841 ttaaatacgc tccttaatgt aaggtgtacg tatctattca acatttcatc gttaagctgc
      901 ttcaaattta ttactttaga gtcattgatt acttacttta aatttgtttc aaagtcactt
      961 caatcttatt aatatcctta ctattcaaca catcacccat gcgctcgaat tgcaatttat
     1021 cacctaaatc aatttcttgc ttttctccat ctttcaaatt gatagtgatc ttaccgtatt
     1081 tagtcgtacc tttatataaa ccataatttt taactaaatg ttgtctaatt ttgaaatcaa
     1141 gttctttcag tgaaacttct tctttattaa ttgaaaatga gtcaattgat gcatctaaat
     1201 ttccgccata cactttatta acaaataaat gtgtattagt ttcagaagat ttgttatttt
     1261 tcttagtcac accaccaaca gtagataatc taccgtttgg atcaattaat tcttttacca
     1321 caaagacatc tttgccttca atgccttctt tatatttctc tttatcttta cctaataaga
     1381 ataattggtg attttgattt tcttggttaa agcgtacaac gttagaaccg ttataatttt
     1441 caaccttacc actaatatta ctgaattcaa aactttctga tgagtagtat cgatgtaagt
     1501 ctttaatatc atataaatgt tgtactctct cttgcttttc ttttgcttga actgcttggc
     1561 cttctgatgt aatcacacca gtagttaata agcctaatgc caatgttgct ttagctaacg
     1621 tttttaattt cacagtacta ttctcccaat ctatttataa atttgtctta atatattttt
     1681 atatgattaa ttcaattacg taatacgaac aatctactat cattatataa gtgagatact
     1741 gcgctatgaa ttaacctctt tttaactatt tttgaaccaa cgttaagtta gttaatgaaa
     1801 tcctaacagt attaattatt ttttataaca caatactaat tttttaacta ttcacacttt
     1861 ccctcaattt aattatgttt tccacattgt ttcatgtcac gaaaaggaca acgcgcgact
     1921 ataagtatca accgtttttc caaaaacttg atcataaacc cgctcctttt ttcatcataa
     1981 caaaataaaa aagaagctaa gcaatatgtt atcgcttaac ttccctacaa ttactagtct
     2041 gcttgtccaa agattattta tattctagct caacattaat ttctttgatt ttggtaccat
     2101 ctatcgtgtc acccatgcga tgcggttgta gtttttttgt aagttcgaaa gtataaaact
     2161 tatcatcttc cattttaact acaattttac cttttctatt attaacagca ccatataatt
     2221 tttcttcttc catcaatttt tttcttaatt taaagtccaa ctcttttaac gagatttctt
     2281 ctttagtaat tttaaattta ctatctttag acttttccga tgattcatca atttttcttg
     2341 ttactttaac atcgacttct ttactaaatt ctttactatt tgtcttactt aaaccaccaa
     2401 ttgaatattg tcttccatta acagtttcct cctcgatgac taaaaatgca tcttgcttat
     2461 catgctcacc ttgagggtat ttttcaatat ctttaccaag caagccaaca aaataatctt
     2521 ttcgatcctt ccaaacatat actcctttat ctgtcacttt ctgtctatac aaaccatcaa
     2581 gattttttaa ctctatactt ggcttgctat agtagtattt taacgattta taattgtgtt
     2641 gaccttgagt tgattctgcc gcttttactg tttgactttc tgttgttatt acaccagtag
     2701 ctaacaatcc caatactaat gttgctttag ctaacgtttt taatttcata gtactattct
     2761 cccaatctat ttataaattt tgtcttaata tatttttata tgattatttc aattacgtaa
     2821 tacgaacaat ctactatcat tatataagcg taatactaag ctatgaatta acctcttttt
     2881 aactattttt gaaccaatgt taagctaatt aatggaatcc taacagcgtt aatctatttt
     2941 ttaaacttaa cgcagctttt ttaactattc acactttccc tcaatttaac tatgttttcc
     3001 acattgtttc atgtcacgaa aaggacaacg cgcgactata agtatcaact atttccacaa
     3061 gttttattgg tgtttttatt attcatcgat acgcttcatt ttcatctctc caacacaaaa
     3121 aagaagctaa gcaacttatg ttgcctaact cctctatact atccatattt tactattatc
     3181 catatttcat tgaattatct aatgttggct tctatttttt caatatttct accgtcaatg
     3241 acgtcactca tgcgatttgt ttgtaatttt ttattaagtt caaacgtata atagccgcca
     3301 tctttcatta tcacttttat cttactatct ttaggaaact ttttatacag atcaaaattt
     3361 tgaattaaat actgtctcaa tttaaagtcg agttctttaa gtgaaatctc ttctttataa
     3421 atgtagtgta ctctaccgta cgtagcaata ccgtcacctt catctctctt gatttgaaat
     3481 cttggtgcgt ttatataatc ataataagcg tcttgatttt tcttagtgac accaccatat
     3541 gaaaacactg tgccattacg gttttccgct tctttaacaa caaatatgtc taatcccgga
     3601 tttttacgtg ctttaaatct ttcaatatct ttaccaaata tctgtactct tgtgaatttt
     3661 ctatttttat caaagataag gtaatgcttg ccacctttgc tataacgata accagtaaca
     3721 tttttaagtt ccttacttgc gccactatag taatctctta agtcaaagat atcttttgtc
     3781 acattttcat attttgcttt atgttcactc gcatttacag tttgatgcaa tgacgttatt
     3841 gttcctgttg ctaaaatacc taatgctaaa cttgctttcg caattgctgt cattttcata
     3901 gttgtatgct ccattcgtaa ttattagatt tgttcgctta cgtctattga atcatacagc
     3961 tttattatag ttagcgtatt tgacctttca cattaaacca tgtttaataa tcattgaatc
     4021 attattaagt aaattaagga atctataatg ttcgttaaat aaaactgatc ccgttgtgct
     4081 tcacacccga tagataggga tttacagata aattcaggtc tcttccacgt catatttgga
     4141 cccatcgaaa attcgggttc tcaaatcatc gaacataaca aaagaagcta agcaacatgt
     4201 aggccgttgt cacttaactt cttgtttttc cgatgacagc ttctatttag agaatgtcat
     4261 gattatttta tattcacttc aatgttatca atattagtgc catctatgac atctgccatg
     4321 cgattttctt gtaatttttt gtgcaattca aacgtgtact ttccaccgtt tttcatttta
     4381 ataacaattt tacctgaacc aacgttaccg tacagattat ttttttcaat aagttgtttt
     4441 ctcaatttaa aatcaagttc tttcaaggaa atctgttctt tagtaatctt gaattctgaa
     4501 acatcatgag agattgtacc tttattatct tccttagtaa ttcttactcc tgctttgtga
     4561 tcaacttttt tactattact ctttgtgata ccaccgacag aatatttttc cagattgtaa
     4621 ttattttctt ctaaaacgac aaatacatcg acattcctat gtactccttc accatatttt
     4681 ttatcatctt taccaactaa agcaatttta tatatgaaat aatctgggac aacattcata
     4741 aatcttattg tcgtccattt ttttaaaata ataccaatct catttttaaa ttctaaactt
     4801 ggtttcgtat aatacgctct taaatcttta aatttaggat ttatttctgt tggtacttgt
     4861 tttgtggttg gcgattgtgg tgtgtctgat ttagtagatt gcattggttg tggcgtgttt
     4921 gttgatggag gtgttgtcac tttagttgaa ggcggtgttg tcgcatttgc tgtttgttgc
     4981 ggtgcttcta ctttagttga gggcggtgtt gtcgcgtttg gttttgattg cggtgcttct
     5041 attttagttg agggcggtgt tgattgtggt gcttccactt tagtggaaga tggtgttgtc
     5101 gcgtttgctg cttgcgttgt cgttgtgatt acacctgttg ttaaaaggcc tagtgctaaa
     5161 cttgttttag caatcgttgt tattttcata gttgtatgct ccattcgtaa ttattagatt
     5221 tgttcgatta cattcattga atcatacagc tttattatag atggcgtatt gctccattca
     5281 cattaaacct tgtttaacta tatttgaatc atcgttaagt aaattaagaa atccataatg
     5341 ttcgttaaat aaaaatgatt ttgatgtgat tcaacacttg gcacatttga agtttcgtca
     5401 cttttaagac atagaaatgc cacttttaca aacaaatgaa tattcgtctt tttacatcat
     5461 tacgcataat aaaagaagct aagcaacatg taaaccgttg tcacttaact tcttgttttt
     5521 ccgatgacag cttctattta gagaatgtca tgattatttt atattcactt caatgttatc
     5581 aatattagtg cctgccatac gatgctcttg cagttttttg tgtaattcaa acgtatattt
     5641 cccaccgttt ttcattttaa taacgattgt tcctgaaccc atgttaccgt aaagattatg
     5701 tttttcaata agttgttttc tcaatttaaa atcaagctct ttcaaggaaa tctcttcctt
     5761 agtaatcatg tattctgaaa catcgcgtga aatcatacct tgattatctt ttttagtaat
     5821 gcttaattct actttgtgat taactttttt actattagtc ttcgtgatgc caccgacaga
     5881 atattttttc aattgatatt tattgtcttc taaaacgata aatacatcga tattatcgta
     5941 aggtccatct ttatattttt tctcatcttt tccaactaaa gctattttat agatgaacct
     6001 atttggaata acattcataa acctaaccgt cgtccatggt ttgagcataa atccaaactg
     6061 cttttcaaat tcaaaactcg gttttgtata atacgctctt aaatcttcat atttaggagt
     6121 catatctgtt tgtgcttgtt ttatggttgg agattgtggt gtgtctgatt tagtagattg
     6181 cattggttgt ggcgtgtttg ttgatggagg tgttgtcact ttagttttcg gcgttgtgga
     6241 ttcggttgtc gtttgtgatt gttcttgttt aggcgctggc gttgctgata tattaagcgt
     6301 tttctgctct tcttgtttag gttgtgatat tttttctatt ttggaagctg aggttttttc
     6361 ctcattagta tttggtgcct tttcgagttt aggcgtgcgt tcttgtcttg tgttagctgc
     6421 ttgtgttgtc gctgaatttg cacctgctgt tatgtttatc attgctaatc gctctgcttt
     6481 aagcgttggt actttgtcaa ctttagttga ttgtattttt tctgctttga ccgattgcgt
     6541 cgttactgta attgcgcctg ttgttaaaag ccctagtgct aaactggttt tagcaattgt
     6601 tctcattttc ataattgtat gctccaatct atattatatt cgattgtctt tttacgtaat
     6661 ttgaatcata caacatcatt atagatggcg ttctaagata atcacattaa acccctttta
     6721 acaattattg aagtattatt aagtaattta agcaaaaaat aatgagtgag tatgagatta
     6781 atatagcgtt tctatgtgcc tttgaaataa tttttaagca ttaaaaagaa gttaagcaac
     6841 gtttgatcgt cacttaacct ctctatttca atttcaactt atttcgtcat caagtatatg
     6901 tgttatgctt ttataacttt gatttcaatt ctatcaatat ctgtgacatt gataacatcg
     6961 gacatacggt cttcttgtaa ctttttatcc aattcaaatg tatactttcc atagtatttc
     7021 tttttgactg taatttttcc tgtactcatt tcaccgtaaa gaccataatt atcaataagg
     7081 tattttctta atttaaaatc aatctctttc aatgacatcg cttctttatc tattttaaat
     7141 gggaaaaagt cataatcata ttcaccagta tgatcttctt taataactct tgcttctgct
     7201 attaggtcga cagctttatc gtttgcactc gtgatacccc caatagagta ctttgcacct
     7261 tcaaatctct tatcctcatt aacgtaaaat atattaagat tacgatgtac acccgtatga
     7321 taatgttgct tatctttgcc aattaaagca atattattaa cagaattacc atctatgata
     7381 ttcataaatt taatacttgg ttgaatgaaa ctgatataac ctgtcacatt tttatattca
     7441 atactaggtt gattataata agcttttaat tttttgctat tttcacttat tacaataggt
     7501 ttcttttcgg catgaactgg tttttccgtt gtagtgttta cacctgttgc taatattcct
     7561 aataacaaac ttatttttgc aatatttttc attttcatag ttgtatgctc caatctatta
     7621 taattagatt gttttattac gtaatttgaa tcatacaccc atattatagg agctgtattc
     7681 ggatattcac attaacctgt ttttaactat tcataaaata tgattaagct atttaagcaa
     7741 aagataatgc gtggtgcgac tgtttgtgtg agttttgtaa cttttcccct caaaaaaaga
     7801 agctaagcaa cagtacgtcg cttaacttcc atatatttcc ataacaagca ctattcaaca
     7861 tagcgctttg ttgttagaaa gtattatttc atttctacta gaattttatt aatcttagtg
     7921 ccgtcgattg actcacccat acgttctttt tcaagttttt gacttaaatc gattgtatgt
     7981 gttgtgccat cattcattgt aattgtaatt tgaccttgtt taagaccatt tgaatacaag
     8041 ccgtgttgtt taatcgcacg ttctcttaat ctataatcaa gttcttttaa tgagatgtct
     8101 tcttttgaaa tgtagtaaaa atctttaagt acatcttcat tatctttctt gatttctaaa
     8161 attggcgtat tgatataatc aatatactgc acactatttg attttgtaac gccaccaatt
     8221 gagtaatttg ttgcttgtct actgtcacct tctctaagga taaacacatc tatatttgag
     8281 ttttctccgt ctttaaattt atctttatca gatccaagta aagtgattcg agaatgctgt
     8341 tgccctactg tgacttctaa atagtgttta ccctcatcag tatatttaaa tccagtcaca
     8401 tttttacgct ctaaaattgg tttattataa tagtgtttta actctgattc actttgttgt
     8461 ttaacttctg ctttcgcttg tactgattgt acattcgatg taattacacc tgttgctaac
     8521 attcccaatg ctaaacttgc ttttgctatc gctttaaatt tcataatttt tagccccaat
     8581 ctatttaaat ttgatttttc aattacgtaa tatgaacaat ctacctacat tatattgagg
     8641 cgctatcccc tttcaaatta aacctcattt aactaaactt gaatcattgt taaggtaatt
     8701 aataaaatgc taactgttat taatcaattc ttatccgtcg agactctttt ttatacaaat
     8761 cacactttat accattttta ttttaaaaac tgtaaaactt tcagacacaa aacagacatc
     8821 atattgtcgt agtacctgat taaaattttc aatgatatta gtgatacaca tcaactaaaa
     8881 cgcgtcatta cggcattttc aacattaata atatttacgt atttactata cacgtcgaca
     8941 ataaatacag attagaattt tttataaaat caaatgtttt gaaaaccatc aaataagtat
     9001 tatgttatgt atataaagat taggagatga agtgaatgaa tattatgtta acaggtgcta
     9061 caggtcattt aggcacacat attacaaatc aagccattgc aaatcacata gatcattttc
     9121 acattggtgt tagaaatgtt gagaaagttc cagaagattg gcgcggaaaa gttcctgttc
     9181 gacaattaga ttattttaat caagaaagca tggtagaagc atttaaagga atggataccg
     9241 ttgtgtttat tccaagtatt atccatccat cattcaaacg tattcctgaa gtggaaaatt
     9301 tagtatatgc ggcaaagcag agcggcgttg ctcatatcat tttcataggt tattacgcag
     9361 atcagcataa taatccattc catatgagtc cttattttgg ttatgcagca cgtctattgg
     9421 caacaagtgg cattgactat acgtatgtaa gaatggcaat gtacatggat ccacttaaac
     9481 catatttacc agaattgatg aatatgcata aactgattta tccagctggc gatggtcgta
     9541 ttaattatat tactagaaat gatattgcta gaggtgtcat tgctattatt aaaaatccag
     9601 atacttgggg caaacgctac ttattatcag gctacagtta tgatatgaaa gaacttgctg
     9661 caattttatc tgaggcatca ggcacagaaa ttaaatatga gcccgtttca ttagagacat
     9721 ttgcagaaat gtatgatgaa cctaaaggct ttggtgcatt attggcatca atgtacgacg
     9781 caggagcaag aggactatta gaccaagaat ccaatgattt ccaacaatta gtcaatgatc
     9841 aaccacagac actgcaatca tttttacaag aaaatattta ataataaagg agcgttatag
     9901 tgaatatcat ctcaacaatt ttaatcatat ttgtggcatt agagtttttc tatattatgt
     9961 accttgaaac gattgctaca acttccaaaa agactagcga gacatttaat ataagcgtcg
    10021 ataaattgaa agacaaaaat attaacctac ttttgaagaa ccaaggcgta tataacggtt
    10081 taatcggagt tttgctaata tacggtttgt ttatcagcag taatccaaaa gaaatatgcg
    10141 cagctatttt agtgtatatc attggcgttg ctatttatgg tggcctttca agcaatatta
    10201 gtatcttttt caaacaaggc acattgccag tattggcact catatcaatg ctttggtaag
    10261 tattggtgtt tgggggggtg gagatgtagt cggaggtttg gaggatttga gcgaattgtg
    10321 tgtggacttt agactcagag tatttcatcc taattatttc aagcagaggt gacagtagcg
    10381 ttgcctctgt ttccttatag aaaaattatt taatgaagaa aacccatatc tgatttaatt
    10441 ttcagctgat aaatactcca tatattagaa tggctacttt atctattgca tcaatccttt
    10501 aaaacaaaaa acccatgatt tcgaaattcc cgtatgatgg ggttcctact ctcatggatc
    10561 agttaaataa atattatcac tatcagttta ttatttcaat attattaaca atatatgtag
    10621 tcgtaaaagg aaagaggaca tgagaacttc ggtgttgatt ggcattacat aacgcttcca
    10681 aacatattat ttggt
//
LOCUS       DAKTIO010000093        10646 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.093, whole genome shotgun sequence.
ACCESSION   DAKTIO010000093 DAKTIO010000000
VERSION     DAKTIO010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10646
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.093"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(187..852)
                     /locus_tag="O0293_001734"
     CDS             complement(187..852)
                     /locus_tag="O0293_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0280619.1"
                     /translation="MALVVEDIVKNFGEGLSETKVLKGINFEVEQGEFVILNGASGSG
                     KTTLLTILGGLLSQTSGTVLYNDAPLFDKQHRPSDLRLEDIGFIFQSSHLVPYLKVIE
                     QLTLVGQEAGMTKQQSSTRAIQLLKNIGLEDRLNVYPHQLSGGEKQRVAIMRAFMNNP
                     KIILADEPTASLDADRATKVVEMIRQQIKEQQMIGIMITHDRRLFEYADRVIELEDGK
                     ITD"
     gene            complement(852..1907)
                     /locus_tag="O0293_001735"
     CDS             complement(852..1907)
                     /locus_tag="O0293_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0280620.1"
                     /translation="MKLAIKEIMFYKFRYILITLIILLLSIMVLFISGLAQGLGRENI
                     SLFEHFDNDEYVVQKMKEPQIEKSQLSDTQQNQIKKVIHQEPYKMNIQTLKLSNKEQD
                     VITMNDVKQQRIQLKKGDYPKNAHEVAINDKLAADNIRVGDRLHFKNNSTSYRVSGIL
                     NDTMYAHSSIVLLNDNGFNALNKVNTAFYPVKNLTQQQRDELNKINDVQVVSEKDLTG
                     NIASYQAEQAPLNMMIVSLFAITAIVLSAFFYVMTIQKISQIGILKAIGIKTRHLLSA
                     LVLQILTLTIIGVGIAVIIIVGLSFMMPVTMPFYLTTQNILLMVGIFILVAILGASLS
                     FIKLFKVDPIEAIGGAE"
     gene            2043..2717
                     /gene="hssR"
                     /locus_tag="O0293_001736"
     CDS             2043..2717
                     /gene="hssR"
                     /locus_tag="O0293_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding heme response regulator HssR"
                     /protein_id="HCY0280621.1"
                     /translation="MVQCLVVDDDPRILNYIASHLQTEHIDAYTQPSGEAALKLLEKQ
                     RVDIAVVDIMMDGMDGFQLCNTLKNDYDIPVIMLTARDALSDKERAFISGTDDYVTKP
                     FEVKELIFRIRAVLRRYNINSNSEMTIGNLTLNQSYLELQVSNKTMTLPNKEFQLLFM
                     LAARPKQIFTREQIIEKIWGYDYEGDERTVDVHIKRLRQRLKKLNATLTIETVRGQGY
                     KVENHV"
     gene            2710..4083
                     /gene="hssS"
                     /locus_tag="O0293_001737"
     CDS             2710..4083
                     /gene="hssS"
                     /locus_tag="O0293_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme sensor histidine kinase HssS"
                     /protein_id="HCY0280622.1"
                     /translation="MFKTLYARIAIYSITVILFSALISFVLTNVYYHYNLKASNDAKI
                     MKTLKEARQYEQSAKPTHIQQYFKHLGQMNYQIMTIDQKGHKTFYGEPFREDTLSQNA
                     INNVLNNQDYHGIKDKPFALFVTGFFDNVTDNTVGINFKTKDGSIAVFMRPDIGETFS
                     EFRTFLAVLLMLLLFISISLVIASTYSIIRPVKKLKLATERLIDGDFETPIKQTRKDE
                     IGTLQYHFNKMRESLGQVDQMRQHFVQNVSHEIKTPLTHIHHLLSELQQTSDKTLRQQ
                     YINDIYTITTQLSGLTTELLLLSELDNHQHLLFDDKIQVNQLIKDIIRHEQFAADEKS
                     LIILADLESINFLGNQRLLHQALSNLLINAIKYTDVGGAIDIALQHSHNNIIFTISND
                     GSPISPQAEARLFERFYKVSKHDNSNGLGLAITKSIIELHHGTIQFTQSNEYVTTFTI
                     TLPNNSL"
     gene            4246..4689
                     /locus_tag="O0293_001738"
     CDS             4246..4689
                     /locus_tag="O0293_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0280623.1"
                     /translation="MMKLNLFINANETESYIDIHAPKMNDNVQSIINAVNDLDKSHTL
                     VGYIDKEIHIINVSDVITFQVINKNVTAITSNQKFKLKLRLYELEKQLPQHFIRISKS
                     EIVNKYYIEKLLLEPNGLIRMYLKDAHYTYSSRRYLKSIKERLSI"
     gene            4686..5141
                     /locus_tag="O0293_001739"
     CDS             4686..5141
                     /locus_tag="O0293_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HCY0280624.1"
                     /translation="MKNLKNSLFISLIIGLSLSLFFSMLFADGKYYPLNPQSTIGILY
                     YTHFTETTVMLISIILWLLIGVVFFLGDFIFKYTDWSITKATIMHFITTYVGFLPLAM
                     LAGWFPLTVHYLIIFTIIFIVVYVLIWIIQFFKNKNYVDTINKQLKQLK"
     gene            complement(5207..6685)
                     /gene="mqo"
                     /locus_tag="O0293_001740"
     CDS             complement(5207..6685)
                     /gene="mqo"
                     /locus_tag="O0293_001740"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCY0280625.1"
                     /translation="MTTQHSKTDVILIGGGIMSATLGTLLKELSPEKNIKVFEKLAQP
                     GEESSNVWNNAGTGHSALCELNYTKEGKDGTVDCSKAIKINEQYQISKQFWAYLVKTG
                     QLDNPDRFIQAVPHMSFVIGEDNVAFIKSRVATLKKSILFEKMKLSQDEEEMKSWVPL
                     MIEGRKSDEPIALTYDETGTDVNFGALTAKLFDNLEQRGVGIQYKQNVLDIKKQKSGV
                     WLVKVKDLETNETTTYESDFVFIGAGGASLPLLQKTGIKQSKHIGGFPVSGLFLRCTN
                     QEVIDRHHAKVYGKAAVGAPPMSVPHLDTRFVDGKRSLLFGPFAGFSPKFLKTGSHMD
                     LIKSVKPNNIVTMLSAGIKEMSLTKYLVSQLMLSNDERMDDLRVFFPNAKNEDWEVIT
                     AGQRVQVIKDTEDSKGNLQFGTEVITSDDGTLAALLGASPGASTAVDIMFDVLQRCYR
                     DEFKGWEPKIKEMVPSFGYRLTDHEDLYHKINEEVTKYLQVK"
     gene            complement(7066..8664)
                     /locus_tag="O0293_001741"
     CDS             complement(7066..8664)
                     /locus_tag="O0293_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCY0280626.1"
                     /translation="MLVNTFNPFDNLLLSSLIAAIPIVLFLLCLTVFKMKGIYAAITT
                     LVVTLLIAIPFFKLPVGIASGAVVEGFFQGIIPIGYIVMMAVLLYKITVESGQFLTIQ
                     DSITNISQDQRIQVLLIGFAFNAFLEGAAGFGVPIAICALLLTQLGFNPLKAAMLCLV
                     ANAASGAFGAIGIPVGVVETLKLPGDVSVLGVSQSATLTLAIINFIIPFLLIFIIDGF
                     RGVKETLPAILVVSITYTFTQGLLTVFSGPELADIIPPLLTMLALAVFSKKFQPKHIY
                     RVNKDEEIEPAKAHSAKAVLHAWSPFIVLTVIVMIWSAPFFKNLFLPNGALSSLVFKF
                     NLPGTISEVTHKPLVLTLNIIGQTGTAILLTIIITILMSKKVNFKDAGRLFGVTFKEL
                     WLPVLTICFILAISKITTYGGLSAAMGQGIAKAGNVFPVLSPILGWIGVFMTGSVVNN
                     NSLFAPIQASVAQQIGTSGSLLVSANTVGGVAAKLISPQSIAIATAAVKQVGKESELL
                     KMTLKYSVCLLIFICIWTFILSLL"
     gene            8981..>10646
                     /locus_tag="O0293_001742"
     CDS             8981..>10646
                     /locus_tag="O0293_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCY0280627.1"
                     /translation="MIKQINVSNMQKFESQLMKAQSEGYTHVVPYANEIMIYQSMLDA
                     VQLYPKSIVVDYTVDGQYKNDCHYFGQSSINIADWAQNNNYYPNLIYAIQQTLDLIHY
                     YSVETIFDLALLTLLKGDLSIDGHVVFDFKAPIATSASIWETIKTIEDFDMMSQFYLN
                     KMAYIDHHPIPFRNLFIEDSEQLNSPDNWLYSTKFMLPKWLYKIAKQRADNKQLQNFG
                     LYTKQPNVLKDHIVFIGDHHQYIGNSKYLFTYFVKHNPMTACYFVTDDRRGPHFISPK
                     SEKADELINSARVVIVENDIPETLQPNGTLIQLHQGTPIMQLFLDSKEPIKNIETPFY
                     RAKRYNRWLQFDYVIHSADDISHFYQTAFPSHQANVLAYGNPKHQYLLQKSNESTTQQ
                     QYKKSFKINDQKPVLLYAPIGLVSAQQLPLSDALFKAYHVVVQGVDEAILPEEALVAP
                     KYLSAQDLILMSDVVITDYSNIIFDAMAIDKTVALYTPNHSQYIESQGVNEDIWRHLS
                     KIWYTDRQLLINNLISQAIPVIKYPQIQHKEQPLESISQLILSKMTS"
BASE COUNT         3658 a         1901 c         1523 g         3564 t
ORIGIN      
        1 tgtacattat aaaatacata tcaaaaaagc tgatttctat caaataatta atagaaatca
       61 gcttttttac attgcctaag aacttaatgt cccagcctgt tttttgtaaa cccaaatcgt
      121 attctgttat ctaactttta tactactatg tcttaaatca ttaacattta gcgtctttac
      181 aagccactaa tcagttattt tgccatcttc taattcaatc actcgatctg catattcaaa
      241 taatcttcga tcgtgtgtaa tcataatacc aatcatttgt tgttctttaa tttgttgacg
      301 tatcatctca acaacttttg ttgctctatc ggcatctaaa cttgctgtgg gctcatctgc
      361 taaaatgatt ttcggattat tcataaatgc tctcataatc gcaacacgtt gcttttcacc
      421 gccagataac tgatgcggat atacattcaa gcgatcttct aaaccaatat ttttcaaaag
      481 ttgtattgct cttgttgaac tttgttgttt ggtcattccc gcttcttgac ctacgagtgt
      541 caattgctct atcactttta aataaggaac taaatgtgaa gattgaaaaa taaaaccaat
      601 atcttccaat cgtaaatcac taggacgatg ctgtttatca aacaatggcg catcattgta
      661 aagcactgta ccactcgttt gacttaacaa tccgcctaat atcgttagca atgttgtttt
      721 cccagaacca gaggcaccat ttaaaatgac aaattcccct tgttccactt caaaattaat
      781 accttttaaa acttttgttt cagacaaacc ttctccgaaa tttttgacga tatcttcaac
      841 gactaatgcc attattctgc acctccaatt gcttcgatag gatccacttt aaataatttg
      901 ataaatgata gtgaggcacc taaaatcgct actaatataa atatccccac cattaataaa
      961 atattttgcg ttgttaagta aaaaggcatc gttaccggca tcataaatga tagtcctact
     1021 atgatgatca cagcaatacc taccccaatt attgttagtg ttaaaatttg taaaactaac
     1081 gcactcaata aatgtcttgt cttaatacca attgctttca aaatgccaat ttgtgatatt
     1141 ttttgaatcg tcataacata gaaaaatgca cttagaacga ttgctgtaat agcaaacaaa
     1201 ctaacaatca tcatatttaa cggtgcttgc tctgcttgat aactcgcaat attacctgtt
     1261 aaatcttttt cactcacaac ttgaacgtca tttattttat taagctcatc acgttgttgt
     1321 tgtgttaaat ttttcactgg ataaaatgcc gtattaacct tattcaatgc attaaatccg
     1381 ttatcgttca atagcacaat ggaactatgc gcatacattg tgtcgtttaa aataccagaa
     1441 actctataac tagttgaatt atttttaaaa tgtaatctat ccccgactct aatgttgtct
     1501 gcagctaact tatcattaat agcgacctca tgagcatttt taggataatc acctttttta
     1561 agttgtatgc gttgttgttt aacgtcattc attgtaatca catcttgttc tttattagat
     1621 aatttcaatg tttgaatatt cattttatat ggttcttgat gaattacctt tttaatttga
     1681 ttctgttgcg tatcactaag ttgcgatttc tcaatttgcg gctctttcat tttttgaaca
     1741 acatattcat cattatcaaa gtgttcaaat aacgaaatat tctccctacc aagcccttga
     1801 gctaaaccac taataaataa caccataata cttaataaaa gaatgattaa tgtgattaaa
     1861 atataacgaa atttgtaaaa cataatctct tttatcgcta atttcatatc gattcacttc
     1921 tccctatttc ttctttagcg tcagtataag gttgaaatat gaactcaata tgaacaattg
     1981 tttttttacg caaaaatatt ttacatgtat aataaatgaa aaagataagg gagtttatag
     2041 ctatggtgca atgtcttgtt gtcgatgacg atcctcgaat cctaaattat atagctagcc
     2101 atttacaaac agagcacatt gatgcataca cacaaccaag tggtgaagct gcattaaaac
     2161 ttttagaaaa acagcgtgtc gatattgcag tggtagatat tatgatggat ggtatggacg
     2221 gctttcaatt atgtaataca ttaaaaaatg attatgatat accagttatt atgttaacag
     2281 cgcgggatgc acttagtgac aaagagcgtg cgtttataag cggtactgac gattatgtaa
     2341 ccaaaccctt tgaggttaag gaacttattt ttagaattcg tgctgtatta cgtcgatata
     2401 atatcaattc aaattcagaa atgactattg gcaacttaac gctaaaccaa tcctatttgg
     2461 aactccaagt atctaataaa acgatgacgt taccgaacaa ggaatttcaa ttattattta
     2521 tgcttgcagc acgtcctaag caaatcttta ctcgtgaaca aataatagaa aaaatttggg
     2581 gctatgatta tgaaggagat gagcgaacag ttgacgttca tattaagcga ctacgccaaa
     2641 gattaaaaaa attaaatgcc acacttacaa ttgaaacagt aagaggacaa ggctataagg
     2701 tggagaatca tgtttaaaac actctatgct agaattgcga tttattccat tacggttatt
     2761 ttatttagtg cattaataag ttttgtatta acaaatgttt actatcatta taatttaaaa
     2821 gcatctaatg acgcgaaaat catgaagacg cttaaagaag caagacaata tgaacaatca
     2881 gctaaaccaa cgcacattca acaatatttc aaacatttag ggcaaatgaa ttaccaaata
     2941 atgaccattg atcaaaaagg tcataagaca ttttatgggg agccatttag agaagatact
     3001 ttatcacaaa atgctattaa caacgtatta aataatcaag attaccatgg tattaaagat
     3061 aaaccattcg cattattcgt aactggattt ttcgacaatg taacagataa tactgttggt
     3121 attaacttta agacaaaaga cggttcaata gcagtattta tgcgtccaga tattggagaa
     3181 acatttagcg agtttagaac attcttagcc gtcttgttaa tgttgttatt atttatctcg
     3241 atttcattag ttatcgcatc aacctattca atcattcgtc cagtaaaaaa gttaaagcta
     3301 gcgaccgaac gcttaattga tggtgatttt gaaacaccta tcaaacaaac acgcaaagat
     3361 gaaattggaa cattacagta tcactttaat aagatgagag agtcattggg acaagttgat
     3421 caaatgaggc agcattttgt acaaaatgta tctcatgaaa tcaaaacacc attaacacat
     3481 attcatcatt tattaagcga gttacaacaa acttctgata aaacattaag acaacaatat
     3541 ataaatgata tctataccat tacaacgcag ctgagcggat taacaactga attgttgctc
     3601 ttatccgaat tagataatca ccaacattta ttattcgatg ataagattca agtcaatcaa
     3661 ctcattaaag atatcattcg acatgaacaa tttgctgctg atgaaaagtc gttaatcatt
     3721 ttagctgacc tagaatcaat caatttccta ggaaatcaac gtttgttaca tcaagcattg
     3781 agtaatttat taataaatgc tattaagtat acagatgttg gtggtgccat tgacatcgct
     3841 ttgcaacata gtcacaacaa tatcattttc acaataagta atgatggttc accaattagt
     3901 ccacaagctg aagcacgttt atttgaacgt ttttataaag tgagcaaaca tgataatagc
     3961 aatggtttag gtcttgcaat tacaaaatcc atcattgaat tgcatcatgg tacaatccaa
     4021 ttcacacaaa gtaacgagta tgtaaccacc tttactatta cactgccaaa taattcactt
     4081 taatctctac ctcctgaaaa aacatttctg tttttatttc aggaggtatc ttttatattg
     4141 attagcgctt tctagccact taacatttaa tttctaccat tcaacaaaat ttaaatatat
     4201 cgatggtcat ttatggttta ataattttga aggaggcatt aacctatgat gaaactcaat
     4261 ttatttatca atgcaaatga gacggaatct tatattgata tacatgcacc taaaatgaat
     4321 gataacgttc aaagtattat taatgcagtc aatgatttag acaaatcaca tacattagtc
     4381 ggatacatag acaaagaaat ccatatcatt aatgtatcag atgttataac attccaagtt
     4441 attaataaaa atgtaacagc cattacaagc aatcaaaaat tcaaactaaa attacgactt
     4501 tatgaactag aaaaacagtt accacaacat ttcattcgca tttccaaatc agaaatcgtc
     4561 aacaagtatt acattgaaaa attattatta gagccaaatg gacttatacg tatgtatctt
     4621 aaagatgcac actacaccta ttcttctaga cgttatttaa aatctataaa ggagcgctta
     4681 tcaatatgaa aaatttgaaa aactcattat ttattagctt aatcattggt ttgtcccttt
     4741 cactgttctt tagtatgtta tttgcagacg gcaaatatta tccactgaac ccacaatcaa
     4801 ctattggcat attgtattat acgcatttca cagaaactac cgtaatgttg atttctatca
     4861 ttttatggtt actcataggc gtcgtctttt tccttggaga ttttatcttt aaatacacag
     4921 attggagcat tactaaagca actataatgc atttcataac aacgtatgtc ggattcttgc
     4981 ctttagcaat gcttgccggt tggtttccac taacagtgca ttatctaatc atattcacaa
     5041 ttatcttcat tgtggtttat gtattaattt ggataattca attctttaaa aacaagaact
     5101 acgtagacac tattaacaag caactgaaac aattaaaatg atttttatta accactaaaa
     5161 aacggtaatg tctatcacat taaaaaagac attaccgttt cgtttattat ttaacttgta
     5221 aatacttagt tacttcttca ttaattttat gatataaatc ctcatgatct gttaagcgat
     5281 aaccaaatga cggcaccatt tctttaatct ttggttccca tcctttgaat tcatcacgat
     5341 agcatctctg taaaacatca aacataatat ctacagctgt tgacgcacca ggtgatgcac
     5401 caagtaatgc agctaatgtg ccatcatctg acgtaataac ttcagtacca aattgtaagt
     5461 tacctttaga atcctcagta tccttgatta cttggacacg ttgccctgct gtaatcactt
     5521 cccaatcttc atttttagca tttgggaaaa agactcttaa atcatccata cgctcatcat
     5581 tagataacat caattgtgac actaaatact tcgtaagact catttctttg atacctgcag
     5641 ataacatcgt cacgatatta tttggtttaa ccgatttaat taaatccata tgtgaacctg
     5701 tttttaaaaa tttaggtgag aaacctgcaa atggaccaaa taacaatgaa cgcttgccgt
     5761 ctacaaaacg tgtatctaag tgcggcactg acattggtgg cgcacccact gctgcttttc
     5821 cgtacacttt agcatgatga cgatcaatca cttcttgatt tgtacagcgc aggaataatc
     5881 cacttaccgg gaaaccacca atatgttttg attgtttaat cccagtcttt tggagtaatg
     5941 gtaaactcgc accgccagca ccaataaata caaaatcaga ttcatatgtt gtcgtttcat
     6001 tagtttctaa atctttaact ttaactagcc ataccccaga tttctgtttc ttgatgtcta
     6061 atacattctg cttatattga attcccacac cacgttgctc taaattatca aataactttg
     6121 cagttaacgc accaaagtta acatctgtac cagtttcatc ataagttaaa gcaattggtt
     6181 catcagactt acgaccttca atcattaacg gtacccaaga tttcatttct tcttcatctt
     6241 gcgataattt cattttttcg aataaaatac ttttctttaa cgttgcaaca cgacttttta
     6301 taaaagctac attatcttcg ccaatgacaa aactcatgtg tggcaccgct tgaataaagc
     6361 gatctgggtt atctaattgt cctgttttaa ctaaatatgc ccaaaactgt tttgaaattt
     6421 ggtactgctc atttatctta attgctttac tacaatcaac tgtgccatcc ttaccttctt
     6481 ttgtatagtt caactcgcaa agtgctgaat gccctgtacc ggcattattc catacatttg
     6541 aactctcttc gccaggttgt gctaattttt caaacacttt aatatttttc tcaggtgata
     6601 attctttaag taatgttcct aatgttgcac tcataatacc gccaccaatt aagatgacat
     6661 ctgttttgct atgttgtgtt gtcataacaa atacagtccc cctttttaaa atgaataatg
     6721 gaacgttcga gaaatacgac atattaatac cactttaaat caataagtgt ctcaataggt
     6781 tcccatggtg atgctctatt actatctact atgtatatta atattttata ataacgaaac
     6841 tactttaatt atatacttta tgtgcgatag cacaaagaca aaaccacaaa ccttaacagc
     6901 tatcaaccaa tttaatttga ttgctgatta ctcactattc attttaacat aaagcgctac
     6961 cattattata atgtcaaact tctttcatta atgatccata aaaaaaagat gagatgttag
     7021 aaaacatctc accttctgta tatttatatt ttgaaatacg tttttttata ataatgacaa
     7081 gatgaaagtc caaatacaga tgaatattag taaacataca ctgtatttca atgtcatttt
     7141 taataattct gattccttac caacttgttt tactgctgca gttgcaattg caattgattg
     7201 tggtgaaatc aattttgccg ctacaccacc aactgtatta gcagatacaa gaagtgaacc
     7261 acttgttcca atttgttgtg caacagaagc ttgaattggt gcaaataatg agttgttatt
     7321 tacaactgat cctgtcataa acacacctat ccaacctaaa attggtgata gaactgggaa
     7381 gacattacct gctttagcaa taccttgacc cattgctgca cttaaaccac cataagttgt
     7441 gattttagaa attgctaaga tgaaacaaat tgtaagaact ggtaaccaca actctttaaa
     7501 tgtaacgccg aataatctac ctgcatcttt aaagttaacc tttttagaca ttaaaattgt
     7561 aataataata gttaataaaa tagctgtacc tgtttgtcca ataatattta aagtcaatac
     7621 taatggttta tgcgtaactt cgctgattgt tccaggtaag ttgaatttaa atactaatga
     7681 tgataaagca ccatttggta agaataagtt tttaaagaat ggcgcactcc aaatcattac
     7741 aatgactgtt aatacaatga atgggctcca tgcatgtaat actgcttttg cagaatgtgc
     7801 ttttgcaggt tcaatttctt catctttatt aacacgataa atgtgttttg gttggaattt
     7861 tttagaaaat actgctaatg ctaacattgt taataacggt ggaataatat ctgctaattc
     7921 aggtccactg aatacagtta ataatccttg agtaaatgta taagtgattg aaactactaa
     7981 aattgctggt aatgtttctt taacacctct aaaaccatca ataataaaga taagtaagaa
     8041 tggaataatg aaatttatga ttgccaatgt taaagttgct gattgagaaa cacctaatac
     8101 tgaaacatct ccaggtaatt tcaacgtttc tacaacacct acagggatac caatcgcacc
     8161 aaaagcacca gacgctgcat ttgcgactaa acataacatc gcagctttta atggattaaa
     8221 tcctaattgt gttaataaaa gtgcacaaat tgcaattggt acaccaaatc ctgctgcacc
     8281 ttctaaaaat gcgttgaatg caaatccaat aagtaaaact tgaatacgtt ggtcttgtga
     8341 aatatttgta atactatctt gaattgttaa aaattgtcca gattcaacag taattttgta
     8401 taacaatact gccatcataa cgatatagcc aatcggaatg ataccttgga agaaaccttc
     8461 gactactgca ccagaagcga taccaactgg caatttgaaa aatggtattg caattagtaa
     8521 tgttacaaca agtgttgtaa tagctgcata aatacctttc attttaaaaa cagttaaaca
     8581 tagtaaaaac agtacaatcg gtattgctgc aattaaagac gataataata aattatcaaa
     8641 tgggttaaac gtatttacta acataggctg actcttcttt ccataaagta gtagtgtaat
     8701 tgcttcatta aaatcatgct ctggctatag cattttgtta tcgataacat tttacagaac
     8761 agtgagatta atgtgatttt tttcacaatt aaaatatacc attttttcag actattaaca
     8821 atatcacttt cctatttaat tgttttattt ttcattttaa atctgttcat caaaatgaat
     8881 tgtacttaat gtaatgaagt aacttatact attcatgaat tttacataag accgtgctaa
     8941 aataaggaag atactacgat gaggaatgga agttatagca atgattaaac agataaatgt
     9001 ttctaatatg caaaagtttg aatctcaatt aatgaaagct cagtctgaag gttatacaca
     9061 tgtcgttcca tatgccaatg agataatgat ttatcaatcg atgttagatg cggttcaatt
     9121 atatcctaag tctatcgtgg ttgattatac tgtggatgga caatacaaaa atgattgtca
     9181 ctatttcggt caatcatcga tcaacattgc cgattgggct caaaacaata attattatcc
     9241 taacctgata tatgcgattc aacagactct tgatttaata cattattact ctgtagaaac
     9301 gattttcgat ttagctttat tgacacttct gaaaggtgat ttatcaatcg atggtcatgt
     9361 cgtttttgat tttaaagccc ctatagcaac aagtgcttca atatgggaaa ctattaaaac
     9421 tattgaagat tttgatatga tgtctcagtt ttatttgaat aaaatggctt atattgatca
     9481 tcatcctata ccttttcgca acctttttat cgaggattca gaacaactta attcgccaga
     9541 taattggttg tattctacca agtttatgct acctaaatgg ttatataaaa tagcaaagca
     9601 acgtgctgat aacaaacaat tacaaaactt cggcctgtat acgaagcaac caaacgtttt
     9661 aaaagatcac atcgttttta tcggtgatca tcatcaatat ataggtaatt caaaatattt
     9721 atttacttac ttcgttaaac ataatccaat gactgcctgt tattttgtta ctgacgatcg
     9781 tcgcggacca catttcatat cacctaagtc tgaaaaagct gacgaattga ttaacagcgc
     9841 acgcgtagtt atagttgaaa atgatatacc tgaaacgtta caaccaaacg gtactttaat
     9901 tcaattacat caaggcacac caataatgca attattttta gatagtaaag agcctattaa
     9961 aaatatagaa acaccttttt atcgtgctaa aaggtataat cgctggttgc aattcgatta
    10021 tgttatacat tctgctgatg atattagcca cttttatcaa acggcttttc ctagtcacca
    10081 agcaaatgta ttggcttatg gtaatcctaa acaccaatat ttactacaaa agagtaacga
    10141 aagtactact caacaacagt ataagaaatc gtttaaaata aatgatcaaa aaccagtttt
    10201 attatacgca cctattggat tagtgagtgc gcaacaatta ccactatcag atgctttatt
    10261 caaagcctat catgttgtgg ttcagggtgt tgatgaagcg atcttaccag aagaggcgct
    10321 tgttgcacca aagtatctta gtgctcaaga cttaattcta atgtctgatg tcgttatcac
    10381 tgattactca aatataatat ttgatgcgat ggctatagac aaaacagtag cattatacac
    10441 tccaaatcac agtcaataca ttgagtctca aggtgttaac gaagatattt ggcgtcatct
    10501 atcaaaaatt tggtatacag accgacaatt attaattaat aatttaattt cccaggcaat
    10561 accagtgatt aaatatccac aaatacaaca taaagaacaa ccattagaat ctatttcaca
    10621 acttatattg tctaagatga catcta
//
LOCUS       DAKTIO010000094        10636 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.094, whole genome shotgun sequence.
ACCESSION   DAKTIO010000094 DAKTIO010000000
VERSION     DAKTIO010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10636
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.094"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            157..1779
                     /locus_tag="O0293_001743"
     CDS             157..1779
                     /locus_tag="O0293_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCY0280628.1"
                     /translation="MKKEKVMDWTTFIGTVAVLLFAVIPMMAFPKASEDIITGINSAI
                     SDSIGSIYLFMGLAIFCFVMYIAFGKYGNVTLGKASDKPEFNTFTWAAMLFCAGIGSD
                     ILYWGVIEWAFYYQVPPNGAKSMSDEALQYATQYGMFHWGPIAWAIYVLPALPIGYLV
                     FVKKQPVYKISQACRPILKGQTDKFVGKVVDILFIFGLLGGAATSLALGVPLISAGIE
                     RLTGLDGKNMILRSAILLTITVIFAISSYTGLKKGIQKLSDINVWLSFVLLAFIFIIG
                     PTVFIMETTVTGFGNMLRDFFHMATWLEPFGGIKGRKETNFPQDWTIFYWSWWLVYAP
                     FIGLFIARISKGRRLKEVVLGTIIYGTLGCVLFFGIFGNYAVYLQISGQFNVTQYLNT
                     HGTEATIIEVVHHLPFPSLMIVLFLVSAFLFLATTFDSGSYILAAASQKKVVGEPLRA
                     NRLFWAFALCLLPFSLMLVGGERALEVLKTASILASVPLIVIFIFMMISFLIILGRDR
                     IKLETRAEKLKEVERRSLRIVQVSEEEQDDNL"
     gene            2043..2729
                     /locus_tag="O0293_001744"
     CDS             2043..2729
                     /locus_tag="O0293_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCY0280629.1"
                     /translation="MTNQFKNKQSKLHDSLESITKNLYATPTSELPFDNRFLFKSFIL
                     KRETGNIVIYHSGHLGDSQQDIASLGGVSKVLMNHDHESIGGSNQVEAPYFIHENDVA
                     ALKHKISVQKQFSNRVMLDKDLEVIPAPGHTPGTTLFLWDDGHHRYLFTGDFICFEGK
                     RWRTVILGSSDREKSIQSLEMVKELDFDVLVPWVTIKDEPLVYFVENEYEKREQIQNI
                     IDRVREGENS"
     gene            complement(2917..3480)
                     /locus_tag="O0293_001745"
     CDS             complement(2917..3480)
                     /locus_tag="O0293_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HCY0280630.1"
                     /translation="MTRSQNKQQQLEQAKDIVINSIGQTMDLYGTNRSVGNLYGTMVF
                     EGSMTLDEMRHQLQMSKPSMSAGVKKLQEFDIVKQQFTRGSRKQHFIAEKDFFIFFRN
                     FFTKKFQREIDINVEAVKDAQAIINSLLESSDLTEAETQEAQKIKSQLDHTHVYYEWL
                     EQLTEAIESGEIFKYFPIPEQPSDSEN"
     gene            complement(3569..3664)
                     /locus_tag="O0293_001746"
     CDS             complement(3569..3664)
                     /locus_tag="O0293_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280631.1"
                     /translation="MKNVSIYKTTQELSFVSSLDFYLTKHLTLML"
     gene            3733..5223
                     /gene="betB"
                     /locus_tag="O0293_001747"
     CDS             3733..5223
                     /gene="betB"
                     /locus_tag="O0293_001747"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HCY0280632.1"
                     /translation="MELLKHLSQRQYIDGEWVESANKNTRDIINPYNQEVIFTVSEGT
                     KEDAERAILAARRAFESGEWSQETAETRGKKVRAIADKIKEHREALARLETLDTGKTL
                     EESYADMDDIHNVFMYFAGLADKDGGEMIDSPIPDTESKIVKEPVGVVTQITPWNYPL
                     LQASWKIAPALATGCSLVMKPSEITPLTTIRVFELMEEVGFPKGTINLILGAGSEVGD
                     VMSGHKEVDLVSFTGGIETGKHIMKNAANNVTNIALELGGKNPNIIFDDADFELAVDQ
                     ALNGGYFHAGQVCSAGSRILVQNSIKDKFEQALIDRVKKIKLGNGFDADTEMGPVIST
                     EHRNKIESYMDVAKAEGATIAVGGKRPDRDDLKDGLFFEPTVITNCDTSMRIVQEEVF
                     GPVVTVEGFETEQEAIQLANDSIYGLAGAVFSKDIGKAQRVANKLKLGTVWINDFHPY
                     FAQAPWGGYKQSGIGRELGKEGLEEYLVSKHILTNTNPQLVNWFSK"
     gene            5484..7193
                     /gene="betA"
                     /locus_tag="O0293_001748"
     CDS             5484..7193
                     /gene="betA"
                     /locus_tag="O0293_001748"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HCY0280633.1"
                     /translation="MSNKNKSYDYVIIGGGSAGSVLGNRLSEDKDKEVLVLEAGRSDY
                     FWDLFIQMPAALMFPSGNKFYDWIYSTDEEPHMGGRKVAHARGKVLGGSSSINGMIYQ
                     RGNPMDYEGWAEPEGMETWDFAHCLPYFKKLEKTYGAAPYDKFRGHDGPIKLKRGPAT
                     NPLFQSFFDAGVEAGYHKTPDVNGFRQEGFGPFDSQVHRGRRMSASRAYLHPAMKRKN
                     LTVETRAFVTEIHYEGRRATGVTYKKNGKLHTIDANEVILSGGAFNTPQLLQLSGIGD
                     SEFLKSKGIEPRVHLPGVGENFEDHLEVYIQHKCKEPVSLQPSLDIKRMPFIGLQWIF
                     TRTGAAASNHFEGGGFVRSNNEVDYPNLMFHFLPIAVRYDGQKAAVAHGYQVHVGPMY
                     SNSRGSLKIKSKDPFEKPSIRFNYLSTEEDKKEWVEAIRVARNILSQKAMDPFNGGEI
                     SPGPEVQTDEEILDWVRRDGETALHPSCSAKMGPASDPMAVVDPLTMKVHGMENLRVV
                     DASAMPRTTNGNIHAPVLMLAEKAADIIRGRKPLEPQYIDYYKHGVHDENEGAIEVKP
                     YAK"
     gene            7471..7683
                     /locus_tag="O0293_001749"
     CDS             7471..7683
                     /locus_tag="O0293_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCY0280634.1"
                     /translation="MSFYEYIQTFKDDKTPLGELAIWIKEDDSFPKQEKLTENILSYF
                     HQMSNIDHEFLEIVKRSLSLYDQLKS"
     gene            7964..8407
                     /locus_tag="O0293_001750"
     CDS             7964..8407
                     /locus_tag="O0293_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCY0280635.1"
                     /translation="MKIYDEYRSYVIEELDDCLTIQKNNDTAYYDVLEAINDLSNDSL
                     CVLNHLYINEGQEETFEQKFLRRNKHLKNVDGFKALRFLRPRTAGRHYIIITLWENRQ
                     AFYHWQNSAEYKHTHKHRGTSKGADVKIINRELSYNIRIELADMV"
     gene            8601..10199
                     /locus_tag="O0293_001751"
     CDS             8601..10199
                     /locus_tag="O0293_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HCY0280636.1"
                     /translation="MQKSALFAPEKYNIISEIEKYSHIPDKKAILYHNTEGEDISVTY
                     QQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRT
                     KDLQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVIAGQKDGWISLEAEKENASN
                     KLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWAT
                     AAPGWQKWVWSPFLSVLGSGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMA
                     KLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDT
                     PQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPALFKGYYKEPERTAKAQ
                     TGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVA
                     KPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNS
                     GKIRRVELRDAEIEKWQQQKDSNQ"
     gene            complement(10259..10588)
                     /locus_tag="O0293_001752"
     CDS             complement(10259..10588)
                     /locus_tag="O0293_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280637.1"
                     /translation="MAYSILNLYSILIQYKCKINHKKDIQYLHPRKTLQLPLILKIIL
                     NLALIGIVIIVLTHTEFQLNAGDSNVFLLNWDIRGLTSYIFSFLILVALVSKLILIFI
                     TRRQRTS"
BASE COUNT         3643 a         1583 c         2077 g         3333 t
ORIGIN      
        1 tatataaata ttcaataatt gccaagtttc aaattgtatg aaatttgcat tattattaaa
       61 tgttagttat tgtcaatttt gtgaatcaat ataattatta cattttgaga taaatcgaaa
      121 caggattcat aaaattaata attaggggga gcacaattga aaaaagagaa agttatggac
      181 tggacgacct ttatagggac agtagctgta cttctttttg cagttatacc tatgatggct
      241 tttccaaaag caagtgaaga tatcatcact ggtattaata gtgccatttc tgattcaatt
      301 ggttcgatat atttatttat ggggctggcg attttttgct ttgtaatgta tatcgcattt
      361 ggtaaatatg gtaatgtcac acttggtaaa gcaagtgata aaccagaatt taatacattt
      421 acatgggcgg caatgctgtt ttgtgcaggc ataggctctg atattttata ctggggcgtt
      481 attgaatggg ctttttacta tcaagttcca ccaaatggcg cgaaaagtat gagtgatgaa
      541 gcactccaat atgcgacgca atatggtatg ttccactggg ggccaattgc ttgggctatt
      601 tatgttctac cagcattacc aattggttat ttagtatttg ttaaaaaaca accggtgtat
      661 aaaattagtc aagcttgtcg tccgatttta aaaggtcaaa cagataaatt tgtaggtaaa
      721 gttgtagata tcttatttat ctttggattg ctaggtggtg cggcaacatc actagcgtta
      781 ggtgtgccat taatttctgc aggcatagaa agattaactg gtttagatgg taaaaatatg
      841 attttacgtt cggccatttt attaacaatc acggttatat ttgccattag ttcatataca
      901 ggattgaaaa aaggtattca aaagttaagt gatatcaacg tttggctatc ctttgtactt
      961 ttagccttta tatttattat tggaccgact gtttttatta tggaaacgac agtgacaggg
     1021 ttcggaaata tgttgagaga tttctttcat atggcaacat ggttagaacc attcggtggt
     1081 attaaaggtc gaaaagaaac gaatttccca caagactgga caatattcta ctggtcatgg
     1141 tggttagtat atgcgccatt tatcggttta tttatcgcta gaatttcaaa aggtcgacgc
     1201 cttaaagaag tcgtgctagg aacaattatt tatggaacgc ttggatgcgt attattcttt
     1261 ggtatttttg gtaactatgc tgtgtattta caaatttctg gacagtttaa tgtaacacaa
     1321 tatttaaata cacatggtac agaggcaacc attattgaag tggtgcatca tttaccattc
     1381 ccatcattga tgattgtact attcttagta tctgctttct tattcttagc aacaacattt
     1441 gattcgggtt catatatttt agcggcagca tctcagaaaa aagtggtagg cgaaccatta
     1501 cgtgccaatc gtttattctg ggcatttgca ttgtgcttat tgccattttc attgatgcta
     1561 gttggtggtg aacgtgcatt agaagtattg aaaactgctt caatactggc aagtgtgcca
     1621 ttaattgtta tttttatttt catgatgata tcatttttaa tcattttagg gcgcgataga
     1681 attaaacttg aaacgcgtgc tgaaaaatta aaagaagttg aacgtcgttc attgcgaatc
     1741 gttcaagtat cagaagaaga acaagacgat aatttataat tcaaagcggg tctgggacga
     1801 cgaaatgaat tttgtgaaaa tatcatttct gttccattcc ccttttttta gtagcattgt
     1861 aggatgaact tttaggtttt cattaatgtt gtactaaaag atttaatttt ttagtgctcc
     1921 aagtacttat ttattgtatg aagcatattc taaatcgaag tttgaaagac tctcattgat
     1981 tattaaatta aataaagggt actgcgtatg tacaattcaa attaatcgaa ggatgaaata
     2041 aaatgactaa tcaatttaaa aataaacagt ccaaattaca tgacagttta gaatccatca
     2101 caaaaaactt atatgcgaca cctacttcgg aactgccttt tgataatcga tttctattta
     2161 aatcttttat tttaaaacgt gaaacaggta atattgttat ctatcattcc ggacacttag
     2221 gtgactccca acaagacatt gcatcattag gtggtgtttc aaaagtattg atgaatcatg
     2281 atcatgaatc tataggaggt tctaatcaag ttgaagcccc ttactttata catgaaaatg
     2341 atgtggctgc actgaaacat aagatttctg ttcaaaaaca atttagtaat cgtgtaatgt
     2401 tggataagga tttagaagtt attcccgcgc ctggacatac accagggacg acactatttt
     2461 tatgggatga tggtcatcac cgttacttat ttactggaga ttttatatgt tttgaaggga
     2521 agagatggcg tacagttata ttaggttcaa gtgatagaga aaaatctatt caaagtttag
     2581 agatggttaa agaattagat tttgatgtac ttgtaccttg ggttactatc aaagatgaac
     2641 cgttagttta ttttgtagaa aatgaatatg aaaaacgtga acaaatacaa aatattattg
     2701 atagagtacg tgagggcgag aatagctaat tgaaatatat tggcgaagca atgtaacgaa
     2761 tctaagaaag ccctagaaaa tacctccata attgattgtc atataaaaca aaaacggtaa
     2821 tttctattta ttgagataga aattaccgtt tatttcgtgg acctattgca ttgtttttat
     2881 catgcataat catcattgtc gttgtttgag tcaattttaa ttttcagaat cagaaggctg
     2941 ttctggaatt gggaaatatt tgaaaatttc accgctttca atcgcttcgg ttaactgttc
     3001 taaccattcg taataaacat gtgtatgatc aagctgagat ttaatttttt gtgcctcttg
     3061 tgtttcagct tcagttaaat cactgctttc aagtaatgaa ttgataatag cttgagcatc
     3121 ttttactgct tcgacattga tgtcaatttc acgctggaat tttttagtga aaaagtttcg
     3181 gaaaaagatg aaaaagtctt tctcggcgat aaaatgttgt ttgcggcttc ctctcgtaaa
     3241 ttgttgttta acaatatcaa attcctgcaa tttcttaacg ccagcactca tacttggttt
     3301 gctcatttgc aattgatgac gcatttcatc aagcgtcata ctgccttcaa acaccattgt
     3361 gccatataag tttcctacac ttctattagt gccatacaaa tccattgtct gtccaattga
     3421 attaattaca atatcttttg cttgttctaa ttgttgctgt ttgttctgag aacgagtcat
     3481 cattgcacct ccgtacatca ttttggtcac gttaaaataa atactaatac attataaaac
     3541 cttttctaaa aaaagacatt aaaaatattt aaagcattaa agttaaatgt ttcgttaaat
     3601 aaaaatctaa cgaacttaca aaacttaatt cttgagttgt tttgtaaatt gacacatttt
     3661 tcatttctat gctaacataa gtttgtaaaa ttcgttaaat aaaaatttaa caaacttaac
     3721 ggaggttgtt gaatggaact tttaaaacat ttatctcagc gtcaatatat tgatggtgag
     3781 tgggttgaaa gcgcgaataa aaatacaaga gatattatca atccttacaa tcaagaagtg
     3841 atatttacgg tttctgaagg gacaaaagag gatgcagaac gtgcaatctt agctgcaaga
     3901 cgtgcgtttg agtctggtga atggtcgcaa gaaactgctg aaacaagagg taaaaaagta
     3961 cgtgccatcg cggacaagat taaagaacat cgcgaagcgt tagcacgatt agaaacatta
     4021 gatactggaa aaacgttaga agaatcatat gcagatatgg atgatattca taatgtgttt
     4081 atgtattttg ctggattagc agataaagac ggtggcgaaa tgattgattc accaattcca
     4141 gatacagaaa gcaaaattgt taaagaacca gtaggtgtag ttacacaaat tacaccttgg
     4201 aattatccgt tattacaagc atcatggaaa attgcgccag cgcttgctac gggttgttca
     4261 ctagttatga aaccaagtga aattacacca ttaacaacaa tacgtgtttt tgaattaatg
     4321 gaagaagttg gtttccctaa aggaacaatt aatcttattc taggtgcagg ttctgaagtt
     4381 ggtgacgtaa tgtcaggtca taaagaggtt gaccttgtat catttacagg tggcattgag
     4441 actggtaagc atattatgaa aaatgctgct aataatgtta cgaatattgc cttggaactt
     4501 ggcggtaaaa atccaaacat tatctttgat gatgctgatt ttgaattggc agtagaccaa
     4561 gcgttaaatg gtggatattt ccatgcaggt caagtttgtt cagcaggatc aagaatatta
     4621 gtacaaaaca gtattaaaga caaatttgag caagcactta ttgatcgcgt gaaaaaaatc
     4681 aaattaggta atggttttga tgctgatact gaaatgggac cagtgatttc aacagaacat
     4741 cgtaataaga tcgaatctta tatggatgta gctaaagcag aaggcgcaac aattgctgtt
     4801 ggtggtaaac gtccagatag agatgattta aaagatggtc tattcttcga gccaacagtc
     4861 attacaaatt gtgatacgtc aatgcgtatt gtacaagaag aggttttcgg acctgtcgtt
     4921 actgtagaag gctttgaaac tgaacaagaa gcgattcaat tagcgaatga ttctatatat
     4981 ggtttagcag gtgctgtatt ttctaaagat attggaaaag cacaacgcgt tgctaacaag
     5041 ttgaaacttg gaacggtgtg gattaatgat ttccatccat attttgcaca agcgccatgg
     5101 ggtggataca aacaatcagg tatcggtaga gaattaggca aagaaggctt agaagagtac
     5161 cttgtttcaa aacacatttt aacaaataca aatccacaat tagtgaattg gtttagcaaa
     5221 taaaaattag ataaggtgag tgccattgta agaacacaag acactcactt tgttttgtat
     5281 aagtggcgaa atgttgattg ataatttgga ctaaacgcaa aatgaatcat agattatttc
     5341 attactgtta gtaacaatcg taaaaggaaa agcgagtgtt ttggttagct aagtttagca
     5401 attcaacgat aaccaatcag ccactaacaa atatttcatg caatactcac tttgaaatac
     5461 aacaaacttt ggaggtcata acgatgagta acaaaaacaa atcatatgat tatgtcatca
     5521 ttggaggagg cagtgcaggt tctgtactag gtaatcgtct gagtgaagat aaagataaag
     5581 aagtcttagt attagaagcg ggtcgcagtg attatttttg ggatttattt atccaaatgc
     5641 ctgctgcgtt aatgttccct tcaggcaata aattttacga ttggatttat tcaacagatg
     5701 aagaaccaca tatgggcggt cgtaaagtag cacatgcccg aggtaaagtt ttaggtggat
     5761 caagttcgat taatggcatg atttatcaac gtggtaatcc aatggactat gaaggctggg
     5821 cagaaccaga aggtatggaa acttgggatt ttgcgcactg tttaccgtat tttaaaaaat
     5881 tagaaaaaac atacggtgca gcgccttatg ataaatttag aggccatgat ggaccaatta
     5941 agttaaaacg agggccagca acgaatcctt tattccagtc attctttgat gcaggtgttg
     6001 aagcaggcta tcataaaaca cctgatgtga atggatttag acaagaaggt tttggaccgt
     6061 tcgatagtca agtacatcgt ggtcgccgaa tgtcagcttc aagagcatat ttacatccag
     6121 cgatgaagcg taaaaactta accgttgaaa cacgtgcctt tgtaactgaa attcattatg
     6181 aaggtagaag agcaactggt gttacgtata agaaaaatgg caaactacat accatcgatg
     6241 ctaatgaagt cattttgtct ggtggggcat tcaatacgcc acaattacta caattatctg
     6301 gtatcggtga ttcagagttc ctaaaatcaa aaggcattga gccacgtgtt catttacctg
     6361 gtgtgggtga aaactttgaa gatcacttag aggtatacat tcaacataaa tgtaaggaac
     6421 ctgtatcatt acagccaagc ttagatatca agcgcatgcc atttattggt ttacaatgga
     6481 ttttcacacg tacaggtgca gcagcatcta accattttga aggtggcgga tttgtacgtt
     6541 caaataacga agttgattat cctaacttaa tgttccattt cttaccaata gctgtaagat
     6601 atgatggtca aaaggcagcg gtcgcacatg gttatcaagt tcacgtagga ccaatgtatt
     6661 caaactctcg tggtagcttg aaaatcaaat ctaaagatcc attcgaaaaa ccaagtatcc
     6721 gctttaatta tttatcaaca gaagaagata aaaaagaatg ggtagaagca attcgtgtag
     6781 caagaaatat cttatctcaa aaagcaatgg atccattcaa tggtggagaa atttcaccag
     6841 gaccagaagt gcaaacagac gaagaaattc ttgattgggt tcgtagagat ggtgaaacag
     6901 cattacaccc atcttgtagt gctaaaatgg gtcctgcttc agatccaatg gcagtagttg
     6961 acccgttaac aatgaaagtc cacggtatgg aaaatttacg tgtagttgat gcatcagcta
     7021 tgccacgtac gacaaatggc aatatacacg cacctgtttt aatgttagct gaaaaagcag
     7081 cagacattat acgtggcaga aagcctttag agccacaata tattgattat tataagcatg
     7141 gtgttcatga tgaaaatgaa ggtgcaatcg aagtaaaacc atacgctaaa taaatttata
     7201 tagtagactc ttagtctggc atctattgta ttggtgccag gcttttttgc gttttatcta
     7261 aatgagaagt taatgtgttg ttcaaaaacg gtgtgtgagt atatgaactg aaaatagagt
     7321 taattgtaca ttgtgatgat taattgacct aattggcaga cattaaaaga gtgcacaacc
     7381 ttaaaaagtt cactaaatag acatttaatt gtgttttctt gaaagctttc gtggtaggat
     7441 ttatataagt aaattgagag aaggaatact atgagctttt acgaatatat acaaacattt
     7501 aaagatgata aaacaccatt aggcgaatta gcgatttgga ttaaagaaga tgattcattc
     7561 ccaaaacaag agaagctaac agaaaacatt ttgtcttatt ttcatcaaat gtccaatata
     7621 gatcatgagt ttttagaaat tgtaaaaaga tcactttctc tgtatgatca attaaaatcg
     7681 taaaatcaga aataaagtta gctaaaataa aaggccggtg atttcacaga tgaagttgtc
     7741 ggctattttc atgactaatg ttaaataatg atcatgaaat tgaagacaca aaaatagtgg
     7801 gcgactcctg tcccccattt gcatgaaata ttaatacttt ttacaaagtc ttatctttta
     7861 aaaagaagcc aaactatgat tgtaattaat aaatacggga atgaattagt aatagatata
     7921 aagtaatatg accaaaaata taaatgaaat gaaggcattt attatgaaaa tttatgatga
     7981 atatagaagt tatgtaatag aagaactgga tgattgtctc acaatacaaa aaaataatga
     8041 cactgcatat tacgatgtgc tagaagcgat aaatgattta tcaaatgatt cattatgtgt
     8101 gttaaatcat ttatatatta acgaaggaca agaagaaaca ttcgagcaaa aatttttgcg
     8161 acgtaataag catttaaaaa acgttgatgg atttaaagca ttaaggtttt taagacctag
     8221 aacagctggt agacactata tcattattac gctgtgggaa aataggcaag cattttatca
     8281 ttggcaaaat tcagctgagt acaagcatac acacaagcat cgtggcactt cgaaaggtgc
     8341 agatgtaaaa ataattaatc gtgaattatc atacaatatt cgtattgaat tagctgatat
     8401 ggtctagccc gagatatcaa ccctcaatca tttgaaagtc cttgaatgct acgtcatgtc
     8461 aaagtgtagt gtcgaggact ttggtattta accctattta attgaaaatt ctgtaaagat
     8521 aggcgtatga tgtatataat tcatttataa aattacaatg ttcatcacag gaaaagtgga
     8581 tgtatgaaag gaagttagtt atgcaaaagt cagctctatt cgcgcctgaa aaatataaca
     8641 ttatttcgga aatcgaaaaa tatagccaca taccagataa aaaggcaatt ttatatcata
     8701 atactgaagg cgaagatata tcagtcactt accaacagct cattgaacag tctaataaag
     8761 taggtaatgt tttagcatcg cacggtttat ctaaaggcga taaagtactc attatgatgc
     8821 cacgatctat tgcgacgtat gaattatata ttgctgcttt aaaattaggt gttgcaatca
     8881 ttccatgttc tgaaatgcta agaactaaag atttacaata ccgtatcaca catggtgaaa
     8941 ttaatgcagt tattgcacta gagccattca cagttgaatt tgaaaaaatt aaagaatatg
     9001 atgcgttaac taaatttgtt attgccggtc aaaaagatgg atggatttca cttgaagctg
     9061 aaaaagaaaa cgcaagtaac aaactagaaa tggcagatac gacgcgtgat gatatggcga
     9121 ttctgtcata tacatctggt acaactggca atcctaaagc cgttacacat tgtcatggat
     9181 ggggatttgc acatttacaa atggcaccaa aacattggtt atgtattaat gaagatgact
     9241 tagtttgggc aacagcagca ccaggttggc aaaaatgggt atggagtcct ttcttatctg
     9301 tattaggttc aggtgcaact gcttttgtat ttaatggaag gttcagtcct gaaacatact
     9361 tagaattgct tcaaaaatat caaattaatg ttttatgttg tacaccgaca gaatatcgta
     9421 tgatggctaa attacagaac ttaaatgatt atgacttaac gcatttacat agtgccgttt
     9481 ctgcaggtga gccactaaat cgagaagtag ttgaacaatt caagaaatac tttaatttaa
     9541 ctgtaagaga tggatatggc cagactgaaa gtaccttgct tataggcttt ttaaaagata
     9601 caccacagag gattggatca atgggtaaag gcattccggg tagttcggtc actgtagtag
     9661 atgatgaggg gaatagcgta cctgctaata cgaaagggaa tattgcagta ccacttgatt
     9721 tgccagcact tttcaaaggg tattataaag agccagaacg tacggcgaaa gcgcaaacag
     9781 gtaaatacta catcactggt gacttagctc atgtagatga tgatggttat ttctggtttg
     9841 aaggacgtcg agatgacatt attattagtt caggctatac gattgggcct tttgaagttg
     9901 aagatgcgtt aacgaatcat ccagccgtta aagaatgtgc tgtagtagca aaacctcatg
     9961 atatacgagg aaatattgtt aaagcattcg ttattttaca ggaccatact gcgggtgatg
    10021 atacgttagt taaagaacta caacaatttg ttaaaaatga agttgcacca tataaatatc
    10081 cgagagagat tgaattcgtt gacgacttac caaaaacaaa ctcaggtaaa attcgtcgtg
    10141 ttgaactacg agatgctgaa atagaaaaat ggcaacaaca aaaagatagc aatcaataaa
    10201 catagcaaag cagtccaatg cgtaaggtat gatgatacgc actggactgc ttgtttattt
    10261 atgatgttct ttgtcgtcta gttataaata ttaaaattag tttagaaaca agcgcaacca
    10321 gtattaagaa tgagaaaata taagatgtta aacctcgaat atcccagttt aataaaaaca
    10381 cattactatc accggcatta agttgaaact cagtatgtgt tagaacgata attacaatac
    10441 ctataagtgc aagattcaaa ataattttca atattaaagg taattgtaat gtttttcttg
    10501 gatgcagata ttgaatatcc tttttatgat tgattttaca cttatattgt atcaaaatag
    10561 agtataggtt taaaatgcta taagccaaat gtagattaaa acaatcgtta gctatttgtt
    10621 gtcaaataat tatgat
//
LOCUS       DAKTIO010000095        10507 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.095, whole genome shotgun sequence.
ACCESSION   DAKTIO010000095 DAKTIO010000000
VERSION     DAKTIO010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10507
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.095"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            96..2033
                     /gene="isdB"
                     /locus_tag="O0293_001753"
     CDS             96..2033
                     /gene="isdB"
                     /locus_tag="O0293_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdB"
                     /protein_id="HCY0280638.1"
                     /translation="MNKQQKEFKSFYSIRKSSLGVASVAISTLLLLMSNGEAQAAAEE
                     TGGTNTEAQPKTEAVASPTTTSEKAPETKPVANAVSVSNKEVEAPTSETKEAKEVKEV
                     KAPKETKEVKPAAKATNNTYPILNQELREAIKNPAIKDKDHSAPNSRPIDFEMKKKDG
                     TQQFYHYASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIKLVSYDTVK
                     DYAYIRFSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKTEEDYKAE
                     KLLAPYKKAKTLERQVYELNKIQDKLPEKLKAEYKKKLEDTKKALDEQVKSAITEFQN
                     VQPTNEKMTDLQDTKYVVYESVENNESMMDTFVKHPIKTGMLNGKKYMVMETTNDDYW
                     KDFMVEGQRVRTISKDAKNNTRTIIFPYVEGKTLYDAIVKVHVKTIDYDGQYHVRIVD
                     KEAFTKANTDKSNKKEQQDNSAKKEATPATPSKPTPSPVEKESQKQDSQKDDNKQLPS
                     VEKENDASSESGKDKTPATKPTKGEVESSSTTPTKVVSTTQNVAKPTTASSKTTKDVV
                     QTSAGSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLPQTGEESNKDMTLP
                     LMALLALSSIVAFVLPRKRKN"
     gene            complement(2187..2360)
                     /gene="rpmF"
                     /locus_tag="O0293_001754"
     CDS             complement(2187..2360)
                     /gene="rpmF"
                     /locus_tag="O0293_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCY0280639.1"
                     /translation="MAVPKRRTSKTRKNKRRTHFKISVPGMTECPNCGEYKLSHRVCK
                     NCGSYNGEEVAAK"
     gene            complement(2440..2997)
                     /locus_tag="O0293_001755"
     CDS             complement(2440..2997)
                     /locus_tag="O0293_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HCY0280640.1"
                     /translation="MKWSITQLRKYQGKPFEFDQTVSFDNLKESLDLIDLSPITIQGQ
                     LTIKSTEVVADIHITGTYTMPCARTLVPVKVPLDVTTTEVFDLEGYNQYNDDQDDVDE
                     HYHIIKDGMVNLQDIVEDIVIIEKPMRAYSEQSDQMLTVGNGWEVIDEDQLDELAKQQ
                     EQDDSESRQVDPRLQKLQQLYDKEQ"
     gene            3124..4263
                     /locus_tag="O0293_001756"
     CDS             3124..4263
                     /locus_tag="O0293_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCY0280641.1"
                     /translation="MKSVGLITEYNPFHNGHQYHINQSKKLTNADVTIAIMSGNFVMR
                     GEPAIYNKFTRAKMALSTADLVIELPATASLSSGDHFAELAVKVADYMSVDTIAFGSE
                     NNDIKTLKQLAHSINEIEQSESFSQKVKEGKSYPRIISELLEHHEALASPNNILGISY
                     LKAIAKNAKNINAISIKRENAQHHDSLIQHHQFASGTSIRTSIISQDDHWHHVVPKDI
                     QHLYVTPHITLNQIFPYLKYQIIAMTTDSLKNIYTVTEGFENRLKSNIYEATDFHHFV
                     KLLKTKRYTYTHIQRLLMNVLLNIKPTDVTSNIHAVKVLAMNDRGRQYLKHLKTAFPE
                     RQYITNINKSNAHYFTNEIKATHIYNAISGQQQTDFNTPVIQQYR"
     gene            complement(4325..4807)
                     /gene="coaD"
                     /locus_tag="O0293_001757"
     CDS             complement(4325..4807)
                     /gene="coaD"
                     /locus_tag="O0293_001757"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCY0280642.1"
                     /translation="MEHTIAVIPGSFDPITYGHLDIIERSTDRFDEIHVCVLKNSKKE
                     GTFSLEERMDLIEQSVKHLPNVKVHQFSGLLVDYCEQVGAKTIIRGLRAVSDFEYELR
                     LTSMNKKLNNEIETLYMMSSTNYSFISSSIVKEVAAYRADISEFVPPYVEKALKKKFK
                     "
     gene            complement(4809..5351)
                     /gene="rsmD"
                     /locus_tag="O0293_001758"
     CDS             complement(4809..5351)
                     /gene="rsmD"
                     /locus_tag="O0293_001758"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HCY0280643.1"
                     /translation="MRVIAGKHKSKALESMEGRNTRPTMDKVKEGIFNSLYDVSGIGL
                     DLFAGSGALGIEALSRGMDKVIFVDQNFKAVKVIKSNLANLDLEAQSEVYKNNADRAL
                     KALSKRDIQFDVIFLDPPYNKGLIDKALKLISEFNLLKENGIIVCEFSNHEEIDYQPF
                     NMIKRYHYGLTDTLLLEKGE"
     gene            5421..5810
                     /locus_tag="O0293_001759"
     CDS             5421..5810
                     /locus_tag="O0293_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280644.1"
                     /translation="MKQTFITLGEGLTDLFEFMTMIEYNHQRIDKIIYFHSPQAENKK
                     SSVAIIMNPTTGNHFQAFYIMINAIKYPYPDSNKKFQMINDCAEKFDIPILGIDVQPP
                     QAFHDLSLYYNYLISVLRLQKWIPELQ"
     gene            complement(5813..6067)
                     /locus_tag="O0293_001760"
     CDS             complement(5813..6067)
                     /locus_tag="O0293_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HCY0280645.1"
                     /translation="MNLIPRTSIVVYLKHMKHERQIRKYGHIVHSNRDRKFVIMYVNE
                     QDVDQIVHKLMQLKYVRHIDGSPYKYLKKTYEKEKHEIYN"
     gene            6306..7232
                     /locus_tag="O0293_001761"
     CDS             6306..7232
                     /locus_tag="O0293_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCY0280646.1"
                     /translation="MKKVNRNILNAVLVGAGFLSSLLMINKNKVITKKQTIPAFFKGN
                     APYIFAHRGGMALRPEQTQLAFDYAKQLGVDGFETDVRLTKDQQLIVFHDATVDRTTN
                     GSGKVSAHTLAELKKLDAAYHFKDINGLTPYRGHAHTAILTFDELLKQYPDMYINVDL
                     KDAPESYEGSIAPQIMFDTIAENQAFDRVLVTSFYKEQIVRFNKIAQGSVAIGASQQE
                     VTEAFLKYHLLGGRYYQPLAQTFQMPTHFKGIDLTSSRFIKWLNDMNIIPGYYGVNSI
                     NLMNDLYQKGAHTIVTDRPDLAQQFKQTIPNK"
     gene            complement(7295..7729)
                     /locus_tag="O0293_001762"
     CDS             complement(7295..7729)
                     /locus_tag="O0293_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCY0280647.1"
                     /translation="MINEASLAILDDIDELADMIVASDIYASFEQAKQALENNDEAHL
                     LYQSFLKSKEKYDEVMRFGKYHPDYKKVMLETRQRKRAYEMLDVVMHYKAREMALQHL
                     IDEVVTKIAYAVSEHVKIETGNPFFQTSHSGCATGGSCNCSL"
     gene            complement(7745..8782)
                     /locus_tag="O0293_001763"
     CDS             complement(7745..8782)
                     /locus_tag="O0293_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HCY0280648.1"
                     /translation="MKKLIIRVVGVLFLVGFLIYLFYSPRLKFDVLENPNKGNKVNRS
                     EQVNKSNNHAENPKPKEGVGTWVGKDIKVLTSKFGQADRVYPFRDGYKNYVFKDKNSY
                     YIVSTKREEIVSVYATGEKVNVSPLKIGQHSAEIFNHTSINPEPSFKVDGKKYEFELS
                     DEDLKTQTLIKYGDIYAQVYSDQQSKKVLSVRFLTKEMLADIEPYQLNSNSTSEEHNK
                     RPVEQNPNQLISLYEVTNEMRKLKGLKPLKINSDLAHIASNNLYEATSNGSDSVEFTE
                     DALRGQLDKNHVTYKTTAQNVGYAFNDVPTLIHSWMNSDIHRSRLLNSKYDEMGGDVM
                     RDYYSLIFLEK"
     gene            complement(9109..9570)
                     /locus_tag="O0293_001764"
     CDS             complement(9109..9570)
                     /locus_tag="O0293_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HCY0280649.1"
                     /translation="MGVPILPTISTTCIVISAILIAIGWRLIWKREINKHKNVMLAAA
                     VFALTFFLIYASRTIFIGNTAFGGPASIKKYYTIFLFFHINLATIGGILGLVQIITAF
                     KDKYNVHRKFGPFASVIWFCTAITGVAVYLLLYVLYPGGETTSLIKATFGH"
     gene            complement(9595..10506)
                     /locus_tag="O0293_001765"
     CDS             complement(9595..10506)
                     /locus_tag="O0293_001765"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCY0280650.1"
                     /translation="MSKEHTLSQNISRVNFKELQQIIKMGLVQGNLIPAFAGAWLAVV
                     MTNHSFLSSIPQILLMLFGSTLIMGGACALNNYYDQDIDRIMPSKQNRPTVNNRITDQ
                     NLLLLSFGMMLVGEICLFLLNIPSGVLGLMGIVGYVSYYSIWSKRHTTWNTVIGSFPG
                     AVPPLIGWVAIEGQISLTAIALFLVVFCWQPIHFYALAIKRKDEYALANIPMLPSVKG
                     FKRTRVSMFIWLIILLPVPLLLINLGVVFVVLATLLNLGWIALGLTTFKKNSDQTKWA
                     TQMFIYSLNYLVIFFVLAVIVSLLTLI"
BASE COUNT         3800 a         1854 c         1477 g         3376 t
ORIGIN      
        1 cgttgcaatt aactaacatt tcaatgtaca atacaagtaa tcaattgata atgattatca
       61 gttgataata tacaattagg agttgtttct acaacatgaa caaacagcaa aaagaattta
      121 aatcatttta ttcaattaga aagtcatcac taggcgttgc atctgtagca attagtacac
      181 ttttattatt aatgtcaaat ggcgaagcac aagcagcagc tgaagaaaca ggtggtacaa
      241 atacagaagc acaaccaaaa actgaagcag ttgcaagtcc aacaacaaca tctgaaaaag
      301 ctccagaaac taaaccagta gctaatgctg tctcagtatc taataaagaa gttgaggccc
      361 ctacttctga aacaaaagaa gctaaagaag ttaaagaagt taaagcccct aaggaaacaa
      421 aagaagttaa accagcagca aaagccacta acaatacata tcctattttg aatcaggaac
      481 ttagagaagc gattaaaaac cctgcaataa aagacaaaga tcatagcgca ccaaactctc
      541 gtccaattga ttttgaaatg aaaaagaaag atggaactca acagttttat cattatgcaa
      601 gttctgttaa acctgctaga gttattttca ctgattcaaa accagaaatt gaattaggat
      661 tacaatcagg tcaattttgg agaaaatttg aagtttatga aggtgacaaa aagttgccaa
      721 ttaaattagt atcatacgat actgttaaag attatgctta cattcgcttc tctgtatcaa
      781 acggaacaaa agctgttaaa attgttagtt caacacactt caataacaaa gaagaaaaat
      841 acgattacac attaatggaa ttcgcacaac caatttataa cagtgcagat aaattcaaaa
      901 ctgaagaaga ttataaagct gaaaaattat tagcgccata taaaaaagcg aaaacactag
      961 aaagacaagt ttatgaatta aataaaattc aagataaact tcctgaaaaa ttaaaggctg
     1021 agtacaagaa gaaattagag gatacaaaga aagctttaga tgagcaagtg aaatcagcta
     1081 ttactgaatt ccaaaatgta caaccaacaa atgaaaaaat gactgattta caagatacaa
     1141 aatatgttgt ttatgaaagt gttgagaata acgaatctat gatggatact tttgttaaac
     1201 accctattaa aacaggtatg cttaacggca aaaaatatat ggtcatggaa actactaatg
     1261 acgattactg gaaagatttc atggttgaag gtcaacgtgt tagaactata agcaaagatg
     1321 ctaaaaataa tactagaaca attattttcc catatgttga aggtaaaact ctatatgatg
     1381 ctatcgttaa agttcacgta aaaacgattg attatgatgg acaataccat gtcagaatcg
     1441 ttgataaaga agcatttaca aaagccaata ccgataaatc taacaaaaaa gaacaacaag
     1501 ataactcagc taagaaggaa gctactccag ctacgcctag caaaccaaca ccatcacctg
     1561 ttgaaaaaga atcacaaaaa caagacagcc aaaaagatga caataaacaa ttaccaagtg
     1621 ttgaaaaaga aaatgacgca tctagtgagt caggtaaaga caaaacgcct gctacaaaac
     1681 caactaaagg tgaagtagaa tcaagtagta caactccaac taaggtagta tctacgactc
     1741 aaaatgttgc aaaaccaaca actgcttcat caaaaacaac aaaagatgtt gttcaaactt
     1801 cagcaggttc tagcgaagca aaagatagtg ctccattaca aaaagcaaac attaaaaaca
     1861 caaatgatgg acacactcaa agccaaaaca ataaaaatac acaagaaaat aaagcaaaat
     1921 cattaccaca aactggtgaa gaatcaaata aagatatgac attaccatta atggcattat
     1981 tagctttaag tagcatcgtt gcattcgtat tacctagaaa acgtaaaaac taataaatcg
     2041 tctttatatt taattattaa attaacaaat tttaattggc ggatgaggta tccagttacc
     2101 tcgttcgcca attatttttc gcaatataaa aagtcccact taaaacaatc attttaagcg
     2161 ggacttttta tattgagtaa ctaaaattat ttagctgcta cttcttcgcc attgtaagaa
     2221 ccacagtttt tacatacacg gtgtgataat ttgtattcgc cacagtttgg gcattcagtc
     2281 atacctggta ctgaaatttt gaaatgcgta cgacgtttgt tttttctagt tttagaagtt
     2341 cttctttttg gtactgccat gatatatcct ccttagatta taaacgaaaa atactaaatg
     2401 ttagtttaat taacaacatt atatcattaa ttaaactact tattgctctt tatcatataa
     2461 ttgttgtaat ttttgaagcc ttggatcaac ttgtcgtgat tctgaatcat cttgttcttg
     2521 ctgtttagca agctcatcta attgatcctc atcgattact tcccaaccat tacctactgt
     2581 caacatttgg tcactttgct ctgaataagc tctcattggt ttctcaataa taactatatc
     2641 ctcgacaata tcctgaagat taaccatacc atctttaata atgtgatagt gttcatctac
     2701 atcatcttga tcatcgttat actgattgta cccttctaaa tcaaatactt ctgtagtagt
     2761 tacatctagt gggactttta ctggtacaag agtacgtgca caaggcattg tatacgttcc
     2821 agtaatgtga atatccgcaa cgacttctgt tgacttaatg gttaactgac cttggattgt
     2881 aattggagat aaatcaatta aatctaatga ttcttttaaa ttgtcaaaac tcaccgtttg
     2941 atcaaattca aatggcttac cttgatattt ccttaattgc gtaattgacc atttcatatg
     3001 gcttcacctc taacaagcac aaaatttatt ttaactttct aagaacaagt tgtcaagatt
     3061 ttttcttaac atatcatgtt ctgttacaat attaataatg aataccgaaa ggacgaattg
     3121 caaatgaaaa gcgttggctt aatcacagaa tataatccct ttcataatgg gcatcaatat
     3181 catattaatc aatctaaaaa acttacaaat gctgacgtta ctattgcaat aatgagtggt
     3241 aactttgtca tgcgtggcga accagcaatc tataataagt ttactcgtgc aaaaatggca
     3301 ttatcaacag ctgatttagt tatcgaacta ccagcaactg ccagtttatc atctggcgat
     3361 cattttgccg aactagcagt taaagtcgca gattatatga gtgtcgatac aattgcattt
     3421 ggtagtgaaa ataatgatat caaaacatta aagcaattag cacacagcat taatgaaatt
     3481 gaacaatctg aatccttttc acaaaaagta aaagaaggta aaagctaccc tcgtatcatt
     3541 agcgaattat tagagcatca tgaagcacta gcaagtccta acaacatact tggtattagt
     3601 tacctgaaag caattgctaa aaatgctaaa aacatcaatg caatttctat caaacgagaa
     3661 aatgctcaac atcatgattc attaattcaa caccatcagt ttgcaagtgg tacatctatt
     3721 agaacatcaa tcattagtca agatgatcat tggcatcatg tggtacctaa agatattcaa
     3781 cacttgtacg taacacctca tataacatta aatcaaatat ttccatattt gaaatatcaa
     3841 atcatagcta tgactacaga ttcattaaaa aatatttata ctgttactga aggttttgaa
     3901 aatcgtttga aatctaacat ttatgaagca actgattttc atcattttgt aaaattacta
     3961 aaaacaaagc gttatacata tacgcatatc caaagactgt taatgaatgt attattaaac
     4021 attaaaccta cagatgttac gagtaacatt cacgcagtta aagtattagc gatgaacgac
     4081 cgtggcagac aatatttaaa gcatttaaaa acagcgttcc cagaaagaca atatataacc
     4141 aatattaaca aaagcaatgc acattacttc acaaatgaaa tcaaagcaac acacatatac
     4201 aatgcaataa gcgggcaaca acaaacagat ttcaacacgc ctgtcataca gcaatatcgt
     4261 taacaaatga aaacagtatt ttaggattgt aaaccatgat aaacctaaaa tactgttatt
     4321 tttattactt aaatttcttc ttcaatgcct tttcaacata aggtggaacg aattcagaaa
     4381 tatctgctcg ataagctgca acttctttaa caatacttga acttataaat gaataattag
     4441 tactagacat catatataac gtttcaattt cattgttcaa ctttttattc attgaagtta
     4501 agcgtaattc atattcaaaa tcactgactg ctcttaaacc acgtatgatt gttttagctc
     4561 ctacttgttc acaataatcg actagtaaac cactaaattg atgaaccttg acattaggta
     4621 aatgtttaac agattgttca attaaatcca tacgctcttc taaactaaac gtaccttctt
     4681 ttttactatt tttaagaaca cagacatgaa tttcatcaaa tctatctgta cttctctcaa
     4741 taatgtctaa atgaccataa gtaatggggt caaaactacc cggaatgacc gctattgtat
     4801 gttccatgct attctccctt ttctaataac aatgtgtctg tcaacccata atggtaacgt
     4861 ttaatcatat taaacggttg ataatctatt tcttcatgat tgctaaattc acaaacgatg
     4921 ataccatttt ctttcaataa attaaactct gaaattagtt ttaaagcttt atcaatgaga
     4981 cctttattat aaggtggatc taagaaaatg acatcaaatt gaatatcacg ttttgacaat
     5041 gcttttaaag ctctatctgc attattttta taaacttcag attgtgcctc taaatccaaa
     5101 ttcgcaagat ttgatttaat aacttttaca gctttaaaat tttgatcaac aaagattacc
     5161 ttatccatac ctcgagagag tgcttctatt ccaagcgccc cgcttcctgc aaataaatct
     5221 aaacctatac ctgacacatc atataaacta ttaaagatac cttctttaac tttatccata
     5281 gttggtctcg tattacggcc ttccatactt tctaaagctt tacttttatg tttacctgca
     5341 atgacgcgca tgttgttcac acttccaatt catttagtta tttaatataa tttattgaga
     5401 aaaaggagaa tgataaacca atgaaacaaa catttattac acttggtgaa ggtctaacag
     5461 atttgttcga attcatgacg atgattgaat ataaccatca acgtattgat aaaattatct
     5521 attttcattc accacaagct gaaaataaaa agtcatctgt agcaatcatt atgaacccta
     5581 caactggcaa tcatttccaa gcattttata tcatgataaa cgctattaaa tatccatatc
     5641 cagattcaaa taaaaagttt caaatgataa atgattgtgc tgaaaaattc gacataccaa
     5701 ttttaggtat cgatgtacag ccccctcaag catttcatga tttatcgtta tattataatt
     5761 atttaattag tgtgttaagg ctccaaaaat ggataccaga acttcaataa tattaattat
     5821 atatttcgtg tttctctttt tcgtaagttt tctttaagta tttatatggt gagccatcaa
     5881 tgtgtctaac gtatttaagt tgcattagtt tatgtacaat ttgatcaaca tcttgctcat
     5941 tcacatacat aattacaaat ttacgatctc tatttgaatg aacgatatgt ccatattttc
     6001 ggatttgtcg ttcatgtttc atatgtttta aataaactac aatactagtt cttgggatta
     6061 aattcattta aacctcactc catttatttt cagcattaat catatcatgt ctttattaat
     6121 tttaaaatat taagctacct taattatgtc ttatgtaatt gctcgataaa tgtatttaga
     6181 ttatattaaa catcattata ttaattcaat tctcaagagc atcactatat gattaaccac
     6241 acatttcgct gtagtcagaa ctatgaattt gttaagctat aaaagtaaac gaggaggaat
     6301 tttttatgaa aaaagtaaac agaaacattt taaatgcggt actagtcggt gctggatttt
     6361 taagtagtct attaatgatc aataaaaata aagtgattac taaaaagcaa accatccctg
     6421 cttttttcaa aggcaatgca ccatatatat ttgcgcatcg cggtggtatg gctttaagac
     6481 cagagcaaac gcaacttgca tttgactatg ctaaacaatt aggcgttgat ggctttgaaa
     6541 cagatgtgag actcacaaaa gatcaacagc ttatcgtttt tcatgacgca actgttgata
     6601 gaacgacgaa cggctctgga aaagttagtg cccatacatt agcagaatta aagaaattag
     6661 acgcagctta tcacttcaaa gatatcaatg gactaacacc atatcgtggt catgcacaca
     6721 cagctatact aaccttcgat gagttgctga agcaatatcc tgatatgtat ataaacgtag
     6781 acttaaaaga tgctcctgaa tcctatgaag gctcaatcgc accacaaatt atgtttgata
     6841 caattgcaga aaatcaagca tttgaccgcg ttcttgtgac aagtttttat aaagaacaaa
     6901 tcgttcgttt taataaaatt gcacaaggat ccgtcgcaat tggtgctagc caacaggaag
     6961 ttacagaagc atttttaaaa tatcatttat taggtggtag gtactaccaa ccattagcac
     7021 aaacgttcca aatgcctact cattttaaag gcattgattt gacttcatct cgttttatca
     7081 aatggttaaa tgatatgaat atcattccag gctattatgg cgtaaatagt ataaatctga
     7141 tgaatgattt ataccaaaaa ggtgcacaca cgattgtgac agatcgacca gatttagcac
     7201 aacaattcaa acaaacaatc ccgaataaat aaaaacatcg agtcagatgc tagttagtgg
     7261 tttcaataac cacctttttc tgactcgatg ttttttataa tgaacaatta caggatccgc
     7321 ccgtcgcaca accactatgt gatgtttgaa agaatggatt acctgtttct attttgacat
     7381 gttctgaaac agcgtacgca attttcgtaa caacttcatc tattaaatgt tgaagtgcca
     7441 tttccctagc tttgtaatgc atcacaacgt caagcatctc atatgctctt ttgcgttgtc
     7501 ttgtctctaa cataactttc ttataatcag gatgatattt cccgaatcgc attacttcat
     7561 catatttctc ttttgatttt aaaaatgatt gataaagtaa atgtgcttcg tcattgtttt
     7621 cgagcgcctg tttagcttgc tcaaatgatg cataaatatc tgatgcaact atcatatcag
     7681 ctagttcatc aatatcatct aatatcgcta gtgaagcttc attaatcata aatacccctc
     7741 attgttattt ttctaagaaa attagtgaat aataatctct cattacatct ccacccatct
     7801 catcgtattt tgaatttaat agacgagatc gatgtatatc tgaattcatc caactatgga
     7861 ttaatgttgg tacatcatta aacgcataac caacattttg agcagttgtt ttataagtaa
     7921 cgtgattttt atctaattgc cctcttaatg cgtcctctgt aaattcaaca ctatcagaac
     7981 cattagaggt cgcttcatat aagttattag atgcaatatg tgctaaatcg ctattgattt
     8041 tcaatggttt taatcctttt aattttctca tttcattcgt tacttcataa agagaaatta
     8101 attgatttgg attttgctca actggacgct tattatgctc ttctgacgta gaattagaat
     8161 ttaattgata aggttcaata tctgctaaca tttcttttgt taaaaatcgt acacttagca
     8221 cctttttcga ttgttgatca gaatacactt gtgcatatat gtcgccatat ttaatcagtg
     8281 tttgtgtttt taaatcttca tctgaaagtt caaattcata ttttttacca tcaactttaa
     8341 aggacggttc tggattaata cttgtatgat taaaaatttc tgcagaatgt tgtcctattt
     8401 ttaacggact aacattgact ttctcacctg tagcatacac tgaaacgatt tcttcacgtt
     8461 tagttgaaac aatgtaataa ctgtttttgt ctttaaacac ataatttttg taaccatctc
     8521 taaaagggta gacacgatct gcttgtccaa atttagaagt aagcacttta atatctttac
     8581 ctacccatgt accaacacct tctttaggct ttggattttc agcatggtta tttgatttat
     8641 tcacctgttc agatctattt actttattac ctttattcgg gttctctaat acatcaaatt
     8701 ttaatctcgg tgaataaaaa agatatatta aaaatccaac taaaaataat acaccgacga
     8761 ctcttataat taactttttc atcaatcaac cacctaaaaa gtattaatac tattgtaaaa
     8821 aacaacacat taattagcaa attttcaaca ctgacataac tgtgtcgttt cgataaaaca
     8881 aaatcatctt caggcataat tttaacgtca taaagatttt ccctacactc tatatcataa
     8941 cctatcttta tgttttcagg ttgaatttca ttattaagat taaaatatgt ataaaaaaat
     9001 ggacagttaa ggtatcaaat gaataccatc aactgtccga ctattcttca tcaaaaaacc
     9061 tgataaaaca aattgcctta tcagattagt atcatttgta taagcatatt aatgaccaaa
     9121 tgttgcttta atcagtgatg tcgtttctcc acctggatat aatacatata ataataagta
     9181 aactgctaca cctgtaattg cagtacagaa ccatataact gaagcgaatg gcccgaattt
     9241 gcggtgtaca ttatatttat ctttaaatgc agtaataatt tgaactaggc ctagaatacc
     9301 accaattgtt gctaaattaa tgtggaaaaa taagaaaatc gtataatatt tcttaattga
     9361 tgctggtccg ccaaatgctg tattaccgat aaaaatcgtt ctacttgcat agattaagaa
     9421 aaaagttaaa gcaaaaacag ctgcagctaa cataacattt ttgtgtttat ttatttccct
     9481 tttccaaata agtctccaac caatggcaat taaaattgca ctaatgacaa tacatgtcgt
     9541 actaatcgtt ggtaaaattg gaacgcccat atttttcatc ctaacttaat taatctagat
     9601 caaagtaagt aatgaaacaa tcacagctaa cacgaaaaag atcactaaat aatttagtga
     9661 atatataaac atttgtgttg cccattttgt ttgatctgaa tttttcttaa atgttgttaa
     9721 acctaatgca atccatccta aatttaataa tgtagctaac actacgaata cgacacctaa
     9781 atttattagt aataaaggta ctggcaataa aataatcaac cagataaaca tactgacacg
     9841 tgtacgttta aagcccttaa ctgatggtaa cattggaata tttgcaagtg catattcatc
     9901 tttacgttta atagctaagg cataaaaatg aattggttgc caacaaaata caactaaaaa
     9961 cagcgcaatc gctgttaaac taatttgtcc ttcaattgca acccatccaa ttagtggtgg
    10021 tactgctcca ggaaaactcc caatcactgt gttccatgtt gtatgtcttt tagaccatat
    10081 tgagtaataa gacacataac ctacaatccc cataagacca agtacgcctg atggtatatt
    10141 caataaaaac aaacaaattt ctccaactaa catcatacca aaacttaata gtaataaatt
    10201 ttgatctgta attctattat ttacagttgg tctattttgt ttactaggca taatacgatc
    10261 aatatcttgg tcgtaataat tatttaacgc acatgcgcca cccataatta aagtagatcc
    10321 aaatagcatt aataaaattt gaggtattga tgataagaag gaatgatttg tcattacaac
    10381 tgctagccat gcgcccgcaa aagccggaat taagttacct tgaacaagtc ccattttaat
    10441 tatctgttgc aattctttga agttaactct gctaatattt tgtgacaaag tatgctcttt
    10501 gctcata
//
LOCUS       DAKTIO010000096        10347 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.096, whole genome shotgun sequence.
ACCESSION   DAKTIO010000096 DAKTIO010000000
VERSION     DAKTIO010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10347
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.096"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..588
                     /gene="capA"
                     /locus_tag="O0293_001766"
     CDS             <1..588
                     /gene="capA"
                     /locus_tag="O0293_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     protein CapA"
                     /protein_id="HCY0280651.1"
                     /translation="FLIISAIVTFFVLSPKYQANTQILVNQTKGDNPQFMAQEVQSNI
                     QLVNTYKEIVKSPRILDEVSKDLNDKYSPSKLSSMLTITNQENTQLINIQVKSGHKQD
                     SEKIANSFAKVTSKQIPKIMSVDNVSILSKADGTAVKVAPKTVVNLIGAFFLGLVVAL
                     IYIFFKVIFDKRIKDEEDVEKELGLPVLGSIQKFN"
     gene            604..1290
                     /gene="cap8B"
                     /locus_tag="O0293_001767"
     CDS             604..1290
                     /gene="cap8B"
                     /locus_tag="O0293_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8B"
                     /protein_id="HCY0280652.1"
                     /translation="MSKKENTTTTLFVYEKPKSTISEKFRGIRSNIMFSKANGEVKRL
                     LVTSEKPGAGKSTVVSNVAITYAQAGYKTLVIDGDMRKPTQNYIFNEQNNNGLSSLII
                     GRTTMSEAITSTEIENLDLLTAGPVPPNPSELIGSERFKELVDLFNKRYDIIIVDTPP
                     VNTVTDAQLYARAIKDSLLVIDSEKNDKNEVKKAKALMEKAGSNILGVILNKTKVDKS
                     SSYYHYYGDE"
     gene            1293..2057
                     /gene="cap8C"
                     /locus_tag="O0293_001768"
     CDS             1293..2057
                     /gene="cap8C"
                     /locus_tag="O0293_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8C"
                     /protein_id="HCY0280653.1"
                     /translation="MIDIHNHILPNIDDGPTNETEMMDLLKQATTQGVTEIIVTSHHL
                     HPRYTTPIEKVKSCLNHIESLEEVQALNLKFYYGQEIRITDQILNDIDRKVINGINDS
                     RYLLIEFPSNEVPHYTDQLFFELQSKGFVPIIAHPERNKAISQNLDILYDLINKGALS
                     QVTTASLAGISGKKIRKLAIQMIENNLTHFIGSDAHNTEIRPFLMKDLFNDKKLRDYY
                     EDMNGFISNAKLVVDDKKIPKRMPQQDYKQKRWFGL"
     gene            2077..3901
                     /gene="cap8D"
                     /locus_tag="O0293_001769"
                     /pseudo
     CDS             2077..3901
                     /gene="cap8D"
                     /locus_tag="O0293_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498717.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8D"
     gene            3891..4919
                     /gene="cap8E"
                     /locus_tag="O0293_001770"
     CDS             3891..4919
                     /gene="cap8E"
                     /locus_tag="O0293_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8E"
                     /protein_id="HCY0280654.1"
                     /translation="MFDDKILLITGGTGSFGNAVMKQFLDSNIKEIRIFSRDEKKQDD
                     IRKKYNNSKLKFYIGDVRDSQSVETAMRDVYYVFHAAALKQVPSCEFFPVEAVKTNII
                     GTENVLQSAIHQNVKKVICLSTDKAAYPINAMGISKAMMEKVFVAKSRNIRSEQTLIC
                     GTRYGNVMASRGSVIPLFIDKIKAGEPLTITDPDMTRFLMSLEDAVELVVHAFKHAET
                     GDIMVQKAPSSTVGDLATALLELFEADNAIEIIGTRHGEKKAETLLTREEYAQCEDMG
                     DYFRVPADSRDLNYSNYVETGNEKITQSYEYNSDNTHILTVEEIKEKLLTLEYVRNEL
                     NDYKASMR"
     gene            4932..6041
                     /gene="cap8F"
                     /locus_tag="O0293_001771"
     CDS             4932..6041
                     /gene="cap8F"
                     /locus_tag="O0293_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8F"
                     /protein_id="HCY0280655.1"
                     /translation="MNIVITGAKGFVGKNLKADLTSTTDHHIFEVHRQTKEEELESAL
                     LKADFVVHLAGVNRPEHDKEFSLGNVSYLDHVLDILTRNTKKPAILLSSSIQATQDNP
                     YGESKLQGEQLLREYAEEYGNTVYIYRWPNLFGKWCKPNYNSVIATFCYKIARNEEIQ
                     VNDRNVELTLNYVDDIVAEIKRAIEGTPTIENGVPTVPNVFKVTLGEIVDLLYKFKQS
                     RLDRTLPKLDNLFEKDLYSTYLSYLPSTDFSYPLLMNVDDRGSFTEFIKTPDRGQVSV
                     NISKPGITKGNHWHHTKNEKFLVVSGKGVIRFRHVNDDEIIEYYVSGDKLEVVDIPVG
                     YTHNIENLGDTDMVTIMWVNEMFDPNQPDTYFLEV"
     gene            6045..7169
                     /gene="cap8G"
                     /locus_tag="O0293_001772"
     CDS             6045..7169
                     /gene="cap8G"
                     /locus_tag="O0293_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8G"
                     /protein_id="HCY0280656.1"
                     /translation="MEKLKLMTIVGTRPEIIRLSSTIKACDQYFNQILVHTGQNYDYT
                     LNQIFFDDLELRQPDHYLEAVGSNLGETMGNIIAKTYDVLLREQPDALLILGDTNSCL
                     AAVSAKRLKIPVFHMEAGNRCFDQNVPEEINRKIVDHVSDVNLPYTEHSRRYLLDEGL
                     NKANIFVTGSPMTEVIEAHRDKINHSDVLNKLGLEPQQYILVSAHREENIDNEKNFKS
                     LMNAINDIAKKYKMPVIYSTHPRSWKKIEESKFEFDPLVKQLKPFGFFDYNALQKDAF
                     VVLSDSGTLSEESSILKFPGVLIRTSTERPEVLDKGTVIVGGITYNNLIQSVELAREM
                     QNNNEPMIDAIDYKDTNVSTKVVKIIQSYKDIINRNTWRK"
     gene            7172..7798
                     /locus_tag="O0293_001773"
     CDS             7172..7798
                     /locus_tag="O0293_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HCY0280657.1"
                     /translation="MRIAIEKIIGLLKNQSSKESNVKIHRLAYITNSKFDGNNYIDRW
                     CKIRNSHIGEYSYIGFGSDFNNVEVGRYCSISSDVKIGLGKHPTHFFSSSPIFYSNNN
                     PFNIKQKFIDFNDQPSRTTIKNDVWIGANVIIMDGLTINTGAVIAAGSVVTKNVGAYE
                     VVGGVPAKVIKKRFDNKTIEKLLESKWWEKTPDKLKGFSVEYLNKKDT"
     gene            7803..8912
                     /locus_tag="O0293_001774"
     CDS             7803..8912
                     /locus_tag="O0293_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCY0280658.1"
                     /translation="MRILNIVSSNIVQDPRVLKQIETIKGVTDDYKIVGMNNSQATNK
                     RLENLDCNYRLLGSKVDPKNILSKLIKRIRFATGVIREIKAYKPDVIHANDFDVLLMV
                     YLSNYKKANIVYDAHEIYAKNAFINKVPLISKFVESIEKHIVKHRVNAFVTVSHAAKE
                     YYQSKGYKKEANVITNAPILNDSREFKEIENFKEIVYQGQIVMDRGYEEFIIASSAFK
                     QNAPSFIIRGFGPHEEVIKELISYNPENIRLDKPVEVKELVDKLAESNVGVVLTKPVS
                     INFEYTVSNKIFECIHAGLPVILSPVKEHIYLNEKYKFGIVLKEVTPLEIEKAVRKLR
                     DNHDLFNHLRQNAIKASKILNWQIESERLVELYKF"
     gene            8926..10092
                     /locus_tag="O0293_001775"
     CDS             8926..10092
                     /locus_tag="O0293_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCY0280659.1"
                     /translation="MKFFVLCAIISMNIFIVISTFTKEVLGFPIEPVYYSTMVGIALI
                     TTVFAIYKIIVTQEIPRGLILLIAICLLYLAFYYFSPDKEEKLAKNNILFFLTWAVPA
                     AISGIYIKYINKATVERFFKLVFFIFSVSFIFVILIPKLTGEIPSYINFGLMNYQNAS
                     YLSAFTAGLGIYFIMKGSVKHKWIYVLFTIIDIPIVFIPGGRGGAILLILYGLFAFIL
                     ITFKRGIPIAVKSIMYIFALSISSVLIYFLFTKGSNTRTFSYLQGGTLNLEGTSGRGP
                     IYEKGIYFIQQSSLLGYGPFNYYKLIGNIPHNIIIELILSFGLLGFFIIMICILLLVY
                     KMIRNYDPNTIDLLVMFIAIYPITLLMFSSNYLVVSEFWFVLFYFITKGRRHHG"
     gene            10085..>10347
                     /locus_tag="O0293_001776"
     CDS             10085..>10347
                     /locus_tag="O0293_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HCY0280660.1"
                     /translation="MAKKVFIMDSVKTIIGTLLIALGLQFLAYPIINQRVGNEAFGSI
                     LTIYTIITITSVVLGNTLNNIRLINMNLYKSNHYYWKFASILL"
BASE COUNT         3745 a         1413 c         1971 g         3218 t
ORIGIN      
        1 tttttaatta ttagcgctat tgttacattt ttcgtcttat cacctaaata tcaagctaat
       61 actcaaattt tagtgaatca aactaagggt gacaatcctc agtttatggc gcaagaggtt
      121 caaagtaata ttcaacttgt aaatacgtat aaagaaattg ttaaaagtcc tagaatttta
      181 gatgaggtgt caaaggactt aaatgataag tattcaccat ctaaattgtc gagtatgttg
      241 acaattacaa accaagaaaa tacgcaactt atcaacatcc aagttaaaag tggtcataaa
      301 caagattcgg aaaaaattgc gaatagcttc gctaaagtta caagtaaaca aattccgaag
      361 attatgagtg tggataacgt atcaatttta tctaaagcag acggtacagc agttaaagtc
      421 gcaccaaaaa ctgtagtgaa tctaatcggt gcattctttt taggattagt tgtcgcgctt
      481 atatatatct tcttcaaagt aattttcgat aagcgaatta aagatgaaga agatgtagag
      541 aaagaattag gattgcctgt attgggttca attcaaaaat ttaattaagg atggttgcta
      601 cttatgtcaa aaaaggaaaa tacgacaaca acactatttg tatatgaaaa accaaaatca
      661 acaattagtg aaaagtttcg aggtatacgt tcaaacatca tgttttcaaa agcaaatggt
      721 gaagtaaagc gcttattggt tacttctgaa aagcctggtg caggtaaaag tacagttgta
      781 tcgaatgtag cgattactta tgcacaagca ggctataaga cattagttat tgatggcgat
      841 atgcgtaagc caacacaaaa ctatattttt aatgagcaaa ataataatgg actatcaagc
      901 ttaatcattg gtcgaacgac tatgtcagaa gcaattacgt cgacagaaat tgaaaattta
      961 gatttgctaa cagctggccc tgtacctcca aatccatctg agttaattgg gtctgaaagg
     1021 ttcaaagaat tagttgatct gtttaataaa cgttacgaca ttattattgt cgatacaccg
     1081 ccagttaata ctgtgactga tgcacaacta tatgcgcgtg ctattaaaga tagtctgtta
     1141 gtaattgata gtgaaaaaaa tgataaaaat gaagttaaaa aagcaaaagc acttatggaa
     1201 aaagcaggca gtaacattct aggtgtcatt ttgaacaaga caaaggtcga taaatcttct
     1261 agttattatc actattatgg agatgaataa gtatgattga tattcataac catatattgc
     1321 ctaatatcga tgacggtccg acaaatgaaa cagagatgat ggatctttta aaacaagcga
     1381 caacacaagg tgttacagaa atcattgtaa catcacatca cttacatcct cgatatacca
     1441 cacctataga aaaagtgaaa tcatgtttaa accatattga aagcttagag gaagtacaag
     1501 cactaaatct aaagttttat tatggtcagg aaataagaat taccgatcaa atccttaatg
     1561 atattgatcg aaaagttatt aacggtatta atgattcacg ctatttacta atagaatttc
     1621 catcaaatga agttccacac tatactgatc aattattttt cgaattacag agtaaaggct
     1681 ttgtaccgat tattgcacat ccagagcgga ataaagcaat aagtcaaaac cttgacatac
     1741 tatacgattt aattaacaaa ggtgctttaa gtcaagtgac aacggcgtca ttagcgggta
     1801 tttccggtaa aaaaattaga aaattagcaa ttcaaatgat tgaaaacaat ctgacacatt
     1861 tcatcggttc agatgcgcat aacacagaaa tcagaccgtt cttaatgaaa gacttattta
     1921 atgataagaa attacgtgat tattatgaag atatgaacgg atttattagt aatgcgaagt
     1981 tagttgttga tgataaaaaa attcctaaac gaatgccaca acaagattat aaacagaaaa
     2041 gatggtttgg gttataaaca gcaaatgagg ggttttatgg cacatttatc tgtgaaattg
     2101 cggcttttaa tactagcatt aatcgattca ctgatagtga cattttcagt attcgtaagt
     2161 tattacattt tagaaccgta tttcaaaaca tattctgtca aattattaat attggcagct
     2221 atatcactat tcatatcgca tcatatttca gcatttattt ttaatatgta tcatcgagcg
     2281 tgggaatatg ccagtgtgag tgaattgatt ttaattgtta aagctgtgac gacatctatc
     2341 gttattacga tggtggtcgt gacaattgtt acaggcaata gaccgttttt tagattgtat
     2401 ttaattactt ggatgatgca cttgatttta ataggtggct caaggttatt ttggcgtatt
     2461 tatcggaaat accttggagg taagtcattt aataagaagc caactttagt tgttggtgct
     2521 ggtcaagcag gttcaatgct gattagacaa atgttgaaaa gtgacgaaat gaaacttgaa
     2581 ccggtattag cagtcgatga tgacgaacat aaacgcaata tcacaattac tgagggtgta
     2641 aaagtccaag gtaaaattgc ggatattcca gaactagtga ggaaatataa gattaaaaaa
     2701 atcatcattg caattccaac tattggtcaa gagcgtttga aagaaattaa taatatttgc
     2761 catatggatg gcgttgagtt attgaaaatg ccaaatatag aagacgtcat gtctggtgag
     2821 ttagaagtga accaacttaa aaaagttgaa gtagaagatt tactaggcag agatcctgtt
     2881 gaattagata tggatatgat atcaaatgaa ttgacgaata aaactatttt agttacgggt
     2941 gcaggtggtt caataggatc agaaatttgt agacaagttt gtaatttcta tccagaacgt
     3001 attattctac ttggccatgg tgaaaacagt atttatttaa tcaatcgtga attgcgaaat
     3061 cgcttcggaa aaaaatgttg atatcgttcc tattatagcg gatgtgcaaa atagagcgcg
     3121 tatgtttgaa attatggaaa cgtataaacc atacgcagtt tatcatgcag cagcacacaa
     3181 gcacgtgccg ttaatggaag acaaccctga agaagcagta cgtaataata ttttaggtac
     3241 gaaaaatact gctgaagctg ctaaaaatgc agaggtaaag aaattcgtta tgatttctac
     3301 ggataaagcc gttaatccgc ctaatgtcat gggagcttca aagcgaattg cagaaatgat
     3361 tattcaaagt ttaaatgatg aaacgcatcg aacaaatttt gttgcagtga gatttggtaa
     3421 tgtacttgga tcgagaggat ctgtgattcc acttttcaaa agtcaaattg aagaaggtgg
     3481 gccagttact gtgacacatc ctgaaatgac acgttacttt atgacaattc ctgaagcttc
     3541 tagactagtt ttgcaggcag gggcattagc agaaggtggc gaagtatttg tgctagatat
     3601 gggagaacca gtgaaaattg tagatttggc acgtaattta attaagctaa gtggtaaaaa
     3661 agaagacgac atacgcatta cttatacagg gattagaccc ggcgaaaaaa tgtttgaaga
     3721 gcttatgaat aaagatgagg ttcatcctga acaagtattt gaaaaaattt atcgtggcaa
     3781 agtacaacat atgaaatgta atgaagttga agcgattatt caagacatcg tcaatgactt
     3841 tagtaaagaa aaaattatta actatgccaa tggcaaaaag ggagataatt atgttcgatg
     3901 acaaaatttt attaattact gggggcacag gatcattcgg taatgctgtt atgaaacagt
     3961 ttttagattc taatattaaa gaaattcgta ttttttcacg cgatgagaaa aaacaagatg
     4021 acattcgaaa aaaatataat aattcaaaat taaagttcta cattggtgat gtgcgtgata
     4081 gtcaaagtgt agaaacagca atgcgagatg tttattacgt attccatgca gcagctttaa
     4141 aacaagtgcc gtcatgtgaa ttctttccag ttgaggcagt gaagacaaat attattggta
     4201 cagaaaatgt cttacaaagt gctattcatc aaaatgttaa aaaagtcata tgtttatcta
     4261 cagataaggc agcgtatcct attaatgcta tgggtatttc aaaagcaatg atggaaaaag
     4321 tattcgtagc caaatcaaga aatattcgta gtgaacaaac gcttatttgt ggtacaagat
     4381 acggtaatgt gatggcttca agaggatcag taataccttt gtttatcgac aaaatcaaag
     4441 ctggagaacc tttaacgatt acagatcctg atatgacaag atttttaatg agcttagaag
     4501 atgcggtaga actagttgtt catgcattta agcatgcaga gacaggagat attatggttc
     4561 aaaaagcacc aagctcaacg gtaggggatc ttgcgaccgc attattagaa ttgtttgaag
     4621 ctgataatgc aattgaaatc attggtacgc gacatggaga gaaaaaagca gaaacattgt
     4681 tgacgagaga agaatacgca caatgtgaag atatgggtga ttattttaga gtgccggcag
     4741 actccagaga tttaaattat agtaattatg ttgaaaccgg taacgaaaag attacgcaat
     4801 cttatgaata taactccgat aatacacata ttttaacggt ggaagagata aaagaaaaac
     4861 ttttaacact agaatatgtt agaaacgaat tgaatgatta taaagcttca atgagatagg
     4921 agagattgac gttgaatatt gtaattacag gagcaaaagg ttttgtagga aaaaacttga
     4981 aagcagattt aacttcaacg acagatcatc atattttcga agtacatcga caaactaaag
     5041 aggaagaatt agagtcagca ttgttgaaag cagactttgt cgtgcattta gcgggtgtta
     5101 atcgacctga acatgacaaa gaattcagct taggaaacgt gagttattta gatcatgtac
     5161 ttgatatatt aactagaaat acgaaaaagc cagcgatatt attatcgtct tcaatacaag
     5221 caacacaaga taatccttat ggtgagagta agttgcaagg ggaacagcta ttaagagagt
     5281 atgccgaaga gtatggcaat acggtttata tttatcgctg gccaaattta ttcggcaagt
     5341 ggtgtaagcc gaattataac tcagtgatag caacattttg ttacaaaatt gcacgtaacg
     5401 aagagattca agttaatgat cggaatgttg aactaacgct aaactacgtg gatgatatcg
     5461 tcgctgaaat aaagcgtgct attgaaggaa ctccaacgat tgaaaatggt gtacctacag
     5521 taccaaacgt atttaaagtg acattgggag aaattgtaga tttattatac aagttcaaac
     5581 agtcacgtct cgatcgaaca ttgccgaaat tagataactt gtttgaaaaa gatttgtata
     5641 gtacgtattt aagctatcta cctagtacag actttagtta tcccttactt atgaatgtgg
     5701 atgatagggg ttcttttaca gaatttataa aaacaccgga tcgtggtcaa gtttctgtaa
     5761 atatttctaa accaggtatt actaaaggta atcactggca tcatactaaa aacgaaaaat
     5821 ttctagtcgt atcaggtaaa ggggtaattc gttttagaca tgttaatgat gatgaaatca
     5881 ttgaatatta tgtttctggc gacaaattag aagttgtaga cataccagta ggatacacac
     5941 ataatattga aaatttaggc gacacagata tggtaactat tatgtgggtg aatgaaatgt
     6001 ttgatccaaa tcagccagat acgtatttct tggaggtata gcgcatggaa aaactgaaat
     6061 taatgacaat agttggtaca aggcctgaaa tcattcgttt atcatcaacg attaaagcat
     6121 gtgatcaata ttttaatcag atattagtac acactggtca aaattatgat tatacattga
     6181 atcaaatttt ctttgatgat ttggaattaa gacaaccgga ccactactta gaggcagttg
     6241 gaagtaacct tggagaaacg atggggaata ttattgcgaa gacatatgat gttttattac
     6301 gcgaacaacc agatgcactt ttaattcttg gtgatacaaa tagttgttta gcagcagtat
     6361 ctgctaaacg attaaagatt cctgtgttcc acatggaagc gggtaataga tgctttgatc
     6421 agaatgtacc tgaagaaatc aatcgtaaaa ttgttgacca tgtcagtgat gtgaatctac
     6481 cttatacgga acatagcaga cgttatttat tagatgaagg cctcaataaa gcgaatatct
     6541 ttgtgacagg atcaccgatg acagaagtga tagaagcgca tcgagataaa attaatcaca
     6601 gtgacgtttt aaataaacta ggattagaac cgcaacaata cattttagta tctgcgcata
     6661 gagaagagaa tatcgataat gaaaagaatt ttaaatcatt aatgaatgcg ataaatgata
     6721 ttgccaaaaa gtataaaatg cctgtgattt attcaacgca tccaagaagt tggaagaaaa
     6781 ttgaagaaag taaatttgaa tttgatccat tagttaaaca gttaaagcca tttggtttct
     6841 ttgattataa tgcattgcaa aaagatgcat ttgttgtgct atcagatagt ggaacattgt
     6901 cagaagagtc gtctattttg aagttccctg gtgtccttat tcgaacttcc acagaaagac
     6961 cggaagtact agataaaggt acggttattg taggtggtat tacctataac aatctaatcc
     7021 aatccgttga actagcaaga gagatgcaaa acaataacga accgatgatt gatgctattg
     7081 attataaaga cactaacgtt tcgacaaagg tagttaaaat tattcaaagc tataaagata
     7141 ttatcaatcg aaatacttgg aggaaatgac gatgaggata gcgattgaaa agataattgg
     7201 tttgctgaaa aaccagtcct ctaaagaatc gaatgttaag attcatcgct tggcgtatat
     7261 tacaaactca aaatttgatg gcaataacta tatagataga tggtgtaaaa tcaggaattc
     7321 tcacattggt gaatacagtt atattggatt tggtagtgat tttaataatg tagaagtagg
     7381 aagatattgt tcgatatctt cggatgtaaa aattgggtta ggaaaacatc ctacacactt
     7441 ttttagctca tcaccgattt tttattctaa taataatcca tttaacataa agcaaaagtt
     7501 tatagacttt aatgaccaac caagccgtac aacaattaaa aatgatgtgt ggattggtgc
     7561 aaatgtaatt attatggatg gtttaacaat aaatactggt gcagtcatag cagccggctc
     7621 agttgttact aaaaatgtag gagcatatga ggttgttggt ggtgttcctg caaaagtgat
     7681 taagaagcga tttgacaata aaacaattga aaaacttttg gaaagcaagt ggtgggagaa
     7741 aacgcctgac aaactaaaag gattttcggt tgaatattta aataaaaagg atacttaatg
     7801 atatgagaat tttaaatatt gtatcgagta atattgttca agacccaagg gtacttaaac
     7861 aaatagaaac aattaaaggc gttacggatg attataaaat tgttggaatg aataattcac
     7921 aagctactaa taagcgattg gaaaatttag attgtaatta tcgtttgtta ggtagcaagg
     7981 tagatccaaa aaatattctt tctaaattaa ttaagcgtat aagatttgca acaggtgtta
     8041 tccgagaaat taaagcttat aaacctgacg tgattcatgc aaatgatttc gacgtattat
     8101 taatggtcta tttaagcaat tataaaaaag ctaatattgt ttatgatgcg catgaaatat
     8161 atgcgaaaaa tgcctttatt aataaagttc cacttatttc aaagtttgta gaaagtatag
     8221 aaaaacacat agtaaaacat cgtgttaatg ccttcgtaac agtaagtcat gcagcaaaag
     8281 aatattatca atctaaagga tataagaagg aagcgaatgt tattacgaat gcacctattt
     8341 taaatgatag cagagaattt aaagaaatcg aaaactttaa agaaattgta tatcaaggtc
     8401 aaattgtaat ggacagagga tatgaagagt ttattattgc ttcatcagct tttaaacaaa
     8461 atgctccttc attcataatt cgagggtttg gtccgcatga agaagtgata aaagaactga
     8521 ttagttataa cccggaaaat attaggttgg ataaaccagt tgaagtaaaa gaattggttg
     8581 ataagttagc agaaagtaat gttggtgttg tcttgacgaa accagtatct attaattttg
     8641 aatatacagt atctaataaa atttttgaat gtatacatgc tggtttacca gtaattttat
     8701 ctcctgtcaa agagcatatt tatctcaatg aaaaatataa atttggcatt gttctaaagg
     8761 aagttacgcc gttagaaatt gaaaaggcgg ttagaaaatt aagagataat cacgatttgt
     8821 ttaatcattt acgtcaaaat gcaattaagg cgtctaaaat tttgaattgg caaatagaga
     8881 gtgaacggtt agtagaatta tataaatttt aaagagaggt agactatgaa attttttgta
     8941 ctttgtgcaa ttatcagcat gaacatattt atagtaatct ctacatttac taaagaagta
     9001 ttagggttcc ctatagagcc ggtgtattac tcaaccatgg ttggtatagc attaattact
     9061 acggtgtttg ctatttataa gataattgtc acccaagaaa ttccgcgagg gttaatatta
     9121 ttaattgcta tatgtttgct ttatctagct ttttattatt tttcaccaga taaggaagag
     9181 aaactagcta aaaataatat tctattcttt ttaacatggg cagttccagc ggcaattagt
     9241 ggtatttata ttaaatatat aaacaaggct acggtagaaa gattttttaa attagtattt
     9301 ttcatatttt ctgtttcatt tatttttgta attttaatac caaaacttac aggtgagata
     9361 cctagctata tcaattttgg acttatgaac tatcaaaacg cttcgtacct ttcagcattt
     9421 actgccggat taggcattta tttcattatg aaaggttcag ttaaacataa gtggatatat
     9481 gttctattta caataattga tatccctatt gtgtttatac caggagggcg tggaggtgct
     9541 attttattaa ttctttacgg cttatttgca tttatactta ttacgtttaa aagaggaata
     9601 cctatcgcag taaaaagcat tatgtatatt tttgcattaa gcatatctag tgtattgatt
     9661 tactttcttt ttacaaaagg ttcgaatact agaacatttt catatctaca aggtggaaca
     9721 cttaatttag aaggtacttc tggaagagga ccgatttatg aaaaaggtat ttactttatt
     9781 caacaaagtt cgttattagg ctatgggcca tttaactatt ataaactaat cggaaatata
     9841 ccacataaca tcattattga gttgattcta tcatttggct tattagggtt ttttatcata
     9901 atgatttgca ttttgctact agtttataaa atgattagga actatgatcc aaacactata
     9961 gatttactcg ttatgtttat agcaatctat ccaatcacat tattaatgtt tagttcaaat
    10021 tatttagttg taagtgaatt ttggtttgtg ttgttctatt ttattacaaa aggacggcgt
    10081 catcatggct aagaaagttt ttattatgga tagcgtaaag acaataattg gtacgttgct
    10141 tatagcttta ggattacaat ttttagctta tccaattatt aatcaacgag taggtaatga
    10201 agcgttcggt tctattttaa cgatttatac aataataaca atcacgagtg ttgtattagg
    10261 caatacgctt aacaatatac gattgattaa tatgaatcta tacaaatcca atcattacta
    10321 ctggaaattt gcatcgatac ttttaat
//
LOCUS       DAKTIO010000097        10313 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.097, whole genome shotgun sequence.
ACCESSION   DAKTIO010000097 DAKTIO010000000
VERSION     DAKTIO010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10313
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.097"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(113..673)
                     /locus_tag="O0293_001777"
     CDS             complement(113..673)
                     /locus_tag="O0293_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCY0280661.1"
                     /translation="MNTIRNSICLTIITMVLCGFLFPLAITLIGQIFFYQQANGSLIT
                     YDNRIVGSKLIGQHWTETRYFHGRPSAVDYNMNPEKLYKNGVSSGGSNESNGNTELIA
                     RMKHHVKFGNSNVTIDAATSSGSGLDPHITVENALKQAPRIADARHVSTSRVADLIQH
                     RKQRGVLTNDYVNVLELNIALDKMKD"
     gene            complement(693..2720)
                     /gene="kdpB"
                     /locus_tag="O0293_001778"
     CDS             complement(693..2720)
                     /gene="kdpB"
                     /locus_tag="O0293_001778"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCY0280662.1"
                     /translation="MHHVNKYFNQTMVIEALKMSFYKLNLKQLIKNPIMFVVEVGMIL
                     TLILICFPDIFGTSYLSRGYLITIFIILLITILFANFSEAFAEGRGKAQADSLRQAQS
                     NLTARLIEENGAYRIVNATELKAGQNIRVENGETIPADGVVINGLATVDESAITGESA
                     PVIKESGGDFDGVIGGTLVTSDWLEIRVESEAGTSFLDKMIALVEGAERNKTPNEIAL
                     FTLLTTLTIIFLVVIVTLYPIASYLHLILPIAMLIALTVCLIPTTIGGLLSAIGIAGM
                     DRVTQFNVLAKSGRAVEVCGDVDVMILDKTGTITYGNRIASEFLPVNQQMLEKLIVAA
                     YMSSIYDDTPEGKSIVRLAKQMYINELPKDIDGTYKPFTAETRMSGIITNEISVFKGA
                     PNSMINLVKQQQGNIPLNIESLCMDVSSKGGTPLIVIENNVMLGVIYLKDVIKDGLVE
                     RFTELRKMGIETVMCTGDNALTAATIAKEAGVDRFVAECKPEDKIKVIKDEQAKGHIV
                     AMTGDGTNDAPALAQANIGLAMNSGTISAKEAANLIDLDSNPTKLIEVVKIGKQLLMT
                     RGALTTFSLANDVAKYFAILPALMMSTIPEMTSLNIMHLSSPKSAIISALIFNALIIV
                     ALIPIAMKGVKVKGYSIDRIFINNMLIYGLGGLIVPFLGIKLIDMIVQFFV"
     gene            complement(2739..4415)
                     /gene="kdpA"
                     /locus_tag="O0293_001779"
     CDS             complement(2739..4415)
                     /gene="kdpA"
                     /locus_tag="O0293_001779"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit A"
                     /protein_id="HCY0280663.1"
                     /translation="MEIILFLTMMVMITYVFSGYLYRVALVQSSRVDLIFTRFENMCF
                     KIIGTDLEHMSAKTYVKHFLAFNGFMGFITFVLLIVQQWLFLNPNHNLNQSIDLAFNT
                     AISFLTNSNLQHYNGESDVTYLTQMIVMTYLMFTSSASGYAVCIAMLRRLTGLTNIIG
                     NFYQDIVRFIVRVLLPLSCLISILLMTQGVPQTLHANLMIRTLSGHIQHIAFGPIASL
                     ESIKHLGTNGGGFLAGNSATPFENPNIWSNFIEMGSMMLLPMSMLFLFGRMLSRHGKR
                     VHRHALILFVAMFFIFIAILTLTMWSEYRGNPILANLGIYGPNMEGKEVRFGAGLSAL
                     FTVITTAFTTGSVNNMHDSLTPIGGLGPMVLMMLNVVFGGEGVGLMNLLIFVLLTVFI
                     CSLMVGKTPEYLNMPIGAREMKCIVLVFLIHPILILVFSALAFMIPGASESITNPSFH
                     GISQVMYEMTSAAANNGSGFEGLKDDTTFWNISTGIIMLLSRYIPIILQLMIASSLVN
                     KKSYHQDKYTIAIDKPYFGVSLIVFIVLLSGLTFIPVLLLGPIGEFLTLK"
     gene            complement(4439..4549)
                     /gene="kdpF"
                     /locus_tag="O0293_001780"
     CDS             complement(4439..4549)
                     /gene="kdpF"
                     /locus_tag="O0293_001780"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCY0280664.1"
                     /translation="MLLCCKEVMAMLIVLLLVVIALVLYLFYALIRSEKF"
     gene            4687..7344
                     /locus_tag="O0293_001781"
     CDS             4687..7344
                     /locus_tag="O0293_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HCY0280665.1"
                     /translation="MSNTESLNIGKKRGSLTIYIGYSPGVGKTFEMLSNAIELFQSNV
                     DIKIGYIEPHQRDETNALAEQLPKITTNFTKHGSHHFQYLDVDRIIEESPTIVLIDEL
                     AHTNISRDRHEKRYMDIEEILNHGIDVHTTLNIQHIESLSSQIELMTGVHVKERVPDY
                     FIMSADVLEVVDISPEQLIKRLKAGKVYKKDRLDVAFSNFFTYAHLSELRTLTLRTVA
                     DLMSDKEKVRHNHKTSLKPHIAVAISGSIYNEAVIKEAFHIAQKEHAKFTAIYIDVFE
                     KNRQYKDSQKQVHQHLMLAKSLGAKVKVVYSQTVALGLDEWCKNQDVTKLIIGQHIRN
                     KWRDFFNTPLIDHLMSFEHSYKIEIVPIKQIPVELKMNKSPYRPKGKRFAIDMLKMIL
                     IQIICVMMGLWIYQLDKHESSTIILMIFLIGIILLSIWTRSFIIGFLAAIINVFVFNY
                     FFTEPRYTFEVYRFDYPITFIVSILTSILTSALLKQIKFQYSITKKQLYRTDLLFQFN
                     DSIKQTYTVENLLINAGYQINQLLQQSITIYVINQSKVIKTIPLQNHIDNTTQQHEQA
                     LSWVIKNERQAGATTDTFPGINKWLIPIGTSPIKGILAIDYQSSQVINPYDASILESM
                     LNELSLAVENVTLLKQTRESMLQAERQLTHSNFLRSISHDIRTPLTTIMGNLDILVSH
                     SKDMSIIEKEQLLVHSFQESQYLYLLVTNILSLTKLQSSNVQIKLQPYLVSELVEEID
                     MILERRHLKKRITVSSSVNLQFIHIDSKLILQALFNLIENAVKHTSTDTKINLSIRYA
                     SYEQIEFAVIDEGPGISLEEQQKIFEPFYTGSNKYFKDNQKESMGLGLYLVQTILHKH
                     QSNLQYKPNQPHGSIFYFNIYTDFNEGDV"
     gene            7344..8039
                     /locus_tag="O0293_001782"
     CDS             7344..8039
                     /locus_tag="O0293_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0280666.1"
                     /translation="MQSKILIIEDDHAITHLLDVALTLDYYNVTTADNATQAHFKIQI
                     DKPDVILLDLGLPDKDGLCLISEIRQHTDIPIIVISARQEEQTIIQALDNGANDYMTK
                     PFNVDELRARIRVIERIAKSHQETNIVFTNGLLSIDFGSKSVVINNQEVHLTPNEFSL
                     LELLSNHKGKVLTYEMILKRIYGYVNKTEMPSLRVHMTSLRQKLSQCHEDAKDIIKTH
                     PRIGYQMLQWKEK"
     gene            complement(8408..9928)
                     /locus_tag="O0293_001783"
     CDS             complement(8408..9928)
                     /locus_tag="O0293_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCY0280667.1"
                     /translation="MQNFKELGISDNTVQSLESMGFKEPTPIQKDSIPYALQGIDILG
                     QAQTGTGKTGAFGIPLIEKVVGKQGVQSLILAPTRELAMQVAEQLREFSRGQGVQVVT
                     VFGGMPIERQIKALKKGPQIVVGTPGRVIDHLNRRTLKTDGIHTLILDEADEMMNMGF
                     IDDMRFIMDKIPAVQRQTMLFSATMPKAIQALVQQFMKSPKIIKTMNNEMSDPQIEEF
                     YTIVKELEKFDTFTNFLDVHQPELAIVFGRTKRRVDELTSALISKGYKAEGLHGDITQ
                     AKRLEVLKKFKNDQINILVATDVAARGLDISGVSHVYNFDIPQDTESYTHRIGRTGRA
                     GKEGIAVTFVNPIEMDYIRQIEDANGRKMSALRPPHRKEVLQAREDDIKEKVENWMSK
                     ESESRLKRISTELLNEYNDVDLVAALLQELVEANDEVEVQLTFEKPLSRKGRNGKPSG
                     SRNRNSKRGNPKFDSKSKRSKGYSSKKKSTKKFDRKEKSSGGSRPMKGRTFADHQK"
BASE COUNT         3529 a         1891 c         1500 g         3393 t
ORIGIN      
        1 ctaatttatt cttcctaaag gtacatctaa aaatttaatt aagcagaaag tgcttgaatt
       61 gctaaaaaga caccatgtat aattttatca acatgatgcc tttcatctat aatcaatctt
      121 tcatcttatc aagagcgata tttagttcaa gcacattcac ataatcattt gttaacacac
      181 cacgctgctt acgatgttga atcaagtcgg ccactcttga agtagataca tgacgagcat
      241 cagcaatacg aggtgcttgc ttcaatgcat tttcgaccgt aatatgcgga tctaagcccg
      301 acccagaact tgttgcagca tctattgtta catttgaatt cccaaattta acatgatgtt
      361 tcatgcgtgc tattaattcg gtgtttccat tcgattcatt acttccacct gaagatacgc
      421 cgtttttata taatttttca ggattcatat tataatcaac tgcactcggt ctcccgtgaa
      481 aatatcgtgt ctctgtccag tgctgtccaa tcaattttga tccaactata cgattgtcat
      541 acgtaattaa actgccattt gcttgttgat aaaaaaatat ttgaccaatt aacgtgatag
      601 ctaacgggaa taaaaatcca cataatacca tagttattat cgttaaacaa atactatttc
      661 ttatcgtatt catggtacag gctccttcct ctttacacaa aaaattgtac aatcatatct
      721 attaatttaa tgcctaaaaa cgggacgatt aatccaccta atccataaat caacatatta
      781 tttataaaga ttctatcaat gctgtaaccc tttactttta cacctttcat ggcaattgga
      841 attaaggcaa caatgattaa tgcattgaat atcaaagctg atataattgc agatttaggt
      901 gacgataaat gcataatatt tagtgaagtc atttctggaa ttgttgacat cattagtgca
      961 ggtaaaattg caaagtattt tgctacgtca ttagccaaac taaatgtcgt taatgcacct
     1021 ctcgtcatta ataattgttt gcctattttt acaacctcta ttaactttgt aggattcgaa
     1081 tctaaatcaa ttagattagc tgcctcttta gcactaattg tccctgagtt catagctaat
     1141 cctatattcg cttgtgctag cgcaggtgca tcatttgtac catctcctgt catcgcaaca
     1201 atatggcctt tcgcttgttc atctttgatg actttaattt tatcttcggg tttacactct
     1261 gcaacaaatc tatcaacccc ggcttctttt gcaattgtag ctgctgttaa agcattatca
     1321 cctgtacaca taactgtttc aatccccatt tttctcaatt cagtaaatcg ttctacaaga
     1381 ccatctttaa tcacatcttt taaataaatc acgccaagca tgacattgtt ttcaatgact
     1441 attaatggtg tgccaccttt actcgataca tccatacaga gagactcaat attaagagga
     1501 atattgcctt gttgttgttt gacaagattt atcatactat taggtgcacc tttgaatacc
     1561 gatatttcat ttgtaatgat tccgctcatt ctagtttcag ctgtaaaagg cttatatgtg
     1621 ccatcaatgt ctttaggcag ctcatttata tacatctgct tcgctaatcg tacaatactt
     1681 tttccttctg gcgtatcatc gtagattgat gacatataag cagcgactat caatttttca
     1741 agcatttgtt gattcactgg taaaaattca ctagcgattc gattgccata agtgattgtg
     1801 cctgtcttgt ctaaaatcat tacatcgaca tctccacata cttctacagc acgcccactt
     1861 ttcgctaata cattgaattg agtaacacga tccatgcctg caataccaat cgccgataac
     1921 aaaccaccga ttgtcgttgg tattaaacat actgttaacg caatgagcat cgcaataggt
     1981 aaaattaaat gcaggtaaga tgctattgga tataacgtta caataacgac taaaaatata
     2041 attgttaacg ttgttaataa tgtaaaaagt gcaatttcat ttggtgtttt atttctttcc
     2101 gccccttcaa ctaaggcaat cattttatct aaaaaagatg tacccgcttc actctcaaca
     2161 cgtatttcta accaatcaga tgttacaagt gtaccgccaa tgactccatc aaaatcgcca
     2221 cctgattctt ttatcacagg tgcagactca ccagtaattg cagattcatc aacggttgct
     2281 aatccattta ttacaacgcc atcagcaggg attgtttctc cattttctac ccgaatattt
     2341 tgtccggctt ttaactctgt ggcgttcact atccgatacg caccattttc ttctatcaat
     2401 cgagcagtta aatttgattg tgcttgtctt aaactatcag cttgcgcttt tccacgacct
     2461 tcagcaaagg cttctgaaaa attagcaaac aatatagtta ttaataatat gataaaaatt
     2521 gtaatcaaat aacctcgcga tagatagcta gttccaaata tgtcaggaaa acatattaat
     2581 atcaacgtta aaatcattcc aacctcaacg acaaacatta tcggattttt tattaattgt
     2641 ttaagattca gcttataaaa actcattttc aaagcttcta tcaccattgt ttgattgaaa
     2701 tatttattta catgatgcat tttttccatc cctttacttt attttaaagt taaaaattca
     2761 ccaataggac caagtaatag tactggaata aatgtcaaac cacttagtaa aacgataaat
     2821 acgattagtg atacgccaaa ataaggttta tcaatcgcta ttgtatattt atcttgatgg
     2881 tatgattttt tattcactaa acttgatgca atcattaatt gcaaaataat tggtatataa
     2941 cgagaaagca acataatgat tcctgtagag atattccaga atgttgtatc atctttcagt
     3001 ccttcaaacc ctgatccatt gttcgcagca gctgatgtca tttcatacat aacttgtgaa
     3061 ataccatgaa aagacggatt cgttatactt tcacttgctc caggaatcat aaaagcaagt
     3121 gctgaaaata ctaaaattaa aattgggtgt atgagaaaga ctaagacaat acatttcatt
     3181 tcacgggcgc caattggcat atttaaatat tctggtgttt taccaaccat caaactgcat
     3241 ataaacaccg tcagtaagac aaatatcaat aaattcatga gtcctacgcc ttcgccacca
     3301 aatacaacat ttagcatcat taataccatt ggtcctaatc cacctatagg cgttaagcta
     3361 tcatgcatgt tattaacaga acccgttgta aatgccgtcg taataactgt aaatagtgct
     3421 gacaaacctg ctccaaaccg tacctcttta ccttccatat tcggtccata aatgcctaaa
     3481 ttcgctagta ttggattacc acgatactca ctccacatag ttaatgtaag aattgctata
     3541 aaaatgaaaa acattgcgac aaataatatc aacgcatgac gatgtactcg tttaccatgt
     3601 ctacttaaca tgcgaccaaa taagaacaac attgacatag gaagtaacat catactgccc
     3661 atttctataa aattgctcca aatatttgga ttttcaaaag gtgtcgcaga atttcctgct
     3721 aaaaatcctc caccattcgt accaagatgt tttattgatt caagtgatgc aataggtcca
     3781 aatgcaatat gttgaatatg tccgcttaaa gtccgaatca ttaaattagc atgcaacgtt
     3841 tgtggtacac cttgagtcat caataaaata ctaattaaac atgataatgg taaaagtact
     3901 cggacaataa accgaacaat atcttgataa aaattaccaa tgatattagt taatccagtt
     3961 aaacgtctca acatcgctat acaaacggcg taacctgatg cactagatgt aaacattaaa
     4021 tatgtcatta caatcatttg cgttaaatat gtcacatctg attcaccgtt atagtgttgt
     4081 aaattactat ttgttaaaaa agatattgct gtattaaacg ctaaatctat cgattggttt
     4141 aaattatgat ttggatttaa aaaaagccat tgctgaacta ttagcaatac aaatgttata
     4201 aaccccataa atccattaaa tgccagaaaa tgtttgacat atgttttagc tgacatgtgt
     4261 tctaaatctg tgccgataat tttaaaacac atattttcaa atctagtaaa tattaaatct
     4321 actcttgacg attgcaccaa tgctacgcga tatagatatc cactaaaaac atacgtaatc
     4381 ataaccatca ttgttagaaa caaaattatt tccatgataa ccctcactta atatatttct
     4441 aaaatttttc actacgaatt aaggcataaa ataaatacaa aactaatgca ataactacca
     4501 gtaataaaac gatgagcatt gccataacct ccttacaaca caacaacatc gtaacaactt
     4561 gtttatgaga gaaatattaa ttttcaaact tagttattaa gaaatcatta agatgtgtat
     4621 gcagaaataa attttatagc atttaattgt gaagaatatt atgatattgc tatcgaggtg
     4681 aaggttatgt caaacactga atcgctaaac ataggaaaaa agcgtggatc tttaacaatt
     4741 tacatcggtt acagtccagg tgttggtaaa acatttgaga tgctttcaaa tgccattgaa
     4801 ctatttcaaa gtaacgttga tattaaaata ggatatattg aaccgcatca gcgtgatgaa
     4861 acaaatgcat tagctgaaca attacctaaa atcacaacca attttactaa acatggtagt
     4921 catcattttc aatatttaga tgtcgaccgc ataatcgaag aatcgccgac aatagtactt
     4981 atcgatgagt tagcacatac gaatatttct agagatcgtc atgagaaacg atatatggat
     5041 attgaagaaa ttttaaatca tggtatcgat gttcatacca ctttgaacat tcaacatatt
     5101 gaaagtttaa gtagtcaaat tgaactgatg accggtgtac atgttaaaga acgtgtaccc
     5161 gactatttca taatgagcgc cgatgtatta gaagtcgtag atatctcacc tgaacaatta
     5221 attaaacgct taaaagctgg caaggtatat aaaaaggata ggctagatgt agcatttagt
     5281 aatttcttta cgtatgccca cctaagcgaa ctgcgtacat tgacgttaag aacagttgcc
     5341 gacttgatga gtgataaaga aaaagtccga cacaaccata aaacgtcact caaacctcat
     5401 attgctgtgg caattagtgg gagcatttat aatgaagcag taattaaaga ggcattccat
     5461 attgctcaaa aagaacatgc gaagttcact gctatttata tagatgtatt cgaaaaaaac
     5521 aggcaatata aagatagtca aaagcaagtg catcaacatc tcatgcttgc aaaatcatta
     5581 ggagcaaaag taaaagtagt ttatagccaa accgttgcat taggattaga cgaatggtgt
     5641 aaaaatcaag atgtaaccaa attaattatc ggacaacata ttagaaataa gtggcgagac
     5701 tttttcaata cacctttaat tgaccattta atgtcctttg aacatagcta taaaatcgaa
     5761 atcgttccaa tcaaacaaat acctgttgaa ttgaaaatga acaaatcacc ctatcgtcct
     5821 aaaggcaaac gtttcgccat agatatgtta aaaatgattt tgattcaaat aatttgtgta
     5881 atgatgggac tgtggattta tcaacttgat aagcatgagt ctagtacgat tattttaatg
     5941 atttttctca tcggcatcat tttattatcc atttggacgc ggtccttcat cattggcttt
     6001 ttagcagcaa ttattaacgt atttgttttt aattattttt ttacggaacc tagatataca
     6061 tttgaagtat atcgctttga ctatcctatt acatttatcg ttagcatttt aacaagtatt
     6121 ttaacgagtg ctttattaaa acaaattaaa ttccaatact ccattactaa aaagcaactt
     6181 tatcgtacag atttattatt tcaatttaat gattcgatta aacaaactta tacagttgaa
     6241 aacttactaa taaatgctgg ataccaaatt aatcaattat tgcaacaatc aattactata
     6301 tacgttatca atcaatcgaa agtaattaaa acaataccat tgcaaaacca tatcgataat
     6361 acgactcaac agcatgaaca agcattaagc tgggtaatta aaaatgaaag acaagctggt
     6421 gcaacaacag atacatttcc aggcattaat aaatggttga taccaattgg cacatctccg
     6481 attaaaggca ttctagccat tgattaccaa agttctcaag tgattaatcc atatgatgca
     6541 tcaatattag aatcaatgtt aaatgaactt tctctcgccg ttgaaaatgt gacgttactt
     6601 aaacaaacga gagaatccat gttacaggct gaacgtcaac tgacacattc aaatttttta
     6661 agatcaattt cgcatgacat acgtacaccg ttaacaacaa ttatgggaaa tttagatatt
     6721 ttagtatcac atagtaaaga tatgtctatc atagaaaagg aacaattact tgtacatagt
     6781 tttcaagaaa gtcagtattt atatctatta gtgacaaata ttttatcatt aacaaaatta
     6841 cagtcatcaa atgttcagat aaaattgcaa ccttatctcg ttagtgaatt agtagaagaa
     6901 atcgatatga ttttagaacg tcgccattta aaaaagcgta taactgtatc atcctcagta
     6961 aatttacagt ttatacacat tgacagtaag ttgattttac aagctttatt caatttgatt
     7021 gaaaatgcag ttaaacacac ttctactgac accaaaatca atttatctat tcgttatgct
     7081 agctatgaac aaatcgaatt tgccgttata gacgaaggac ctggaattag tttagaagaa
     7141 caacaaaaga tattcgagcc tttttacaca ggatcaaata aatattttaa agataaccaa
     7201 aaagaaagca tgggactagg cttatattta gtacagacta tcctacacaa acatcaatca
     7261 aacttacaat ataaaccgaa ccaaccacac ggcagtatat tctatttcaa tatatataca
     7321 gactttaacg aaggagacgt ataatgcaat ctaaaatatt gataattgaa gatgatcacg
     7381 caatcacaca tttacttgat gttgcattaa ctttagatta ttacaatgta actacagccg
     7441 acaatgccac acaagcacac tttaaaattc aaattgataa accagatgtc attttattag
     7501 atttaggttt accagataaa gatggattat gtttgatttc agaaatcagg caacatactg
     7561 acattcctat cattgtaata agtgcaagac aagaagaaca aacaattatt caagctttag
     7621 ataacggtgc gaatgactat atgactaaac cttttaatgt tgatgagctt cgggcacgaa
     7681 tacgagtcat cgagagaatc gctaagtcac atcaagaaac taacattgta tttactaacg
     7741 gtttgctaag catcgacttt ggctccaaat ctgttgttat taataatcag gaggtacatt
     7801 tgacgccaaa tgaatttagc ttgttagaac tattgtcaaa tcataaagga aaagtactca
     7861 cttatgagat gatattaaaa cgaatatatg gctatgttaa taagactgaa atgcctagtt
     7921 taagagtgca tatgacatca ttaagacaaa aactttcaca atgtcatgag gacgcaaaag
     7981 atattattaa aacacatcca cgcattggtt atcaaatgtt gcagtggaaa gagaaataat
     8041 taaataaaaa agatcgctgc cattaaacga atgattggca gtgatctttt ttatattcaa
     8101 cttattattt atcgcgcact agcgtcagca aaaagcccta ttgtttttta taagaacatg
     8161 atactgaaat ttcataaagt ttatttatgt tcgattattc ttaatttaaa aatagttctt
     8221 taaattatat actcaccaca tttggtggag aaccatattt acacagaatg atataaccgc
     8281 atacagatct caatccaatt aaatatttaa cctttaaaac gttagtactt tgatatctac
     8341 agagaatcaa ttattgaaaa caaaaaagag ctcaagacat tacatcttta agctcttaat
     8401 ctataaatta tttttgatgg tcagcaaatg tgcgaccttt cataggtctg cttccaccgc
     8461 tgctcttctc tttacggtcg aatttttttg tacttttctt cttacttgag tatccttttg
     8521 aacgtttact cttactgtca aatttaggat taccacgctt actatttctg ttacgagaac
     8581 cacttggttt accgttacgg cctttgcgag ataatggttt ttcaaaagtt aattgaactt
     8641 caacttcatc gtttgcttct actaactctt gtaaaagtgc agcaactaaa tcaacatcgt
     8701 tatattcatt taacaactct gtagaaatgc gtttcaagcg tgattctgac tctttagaca
     8761 tccagttttc aactttttct ttgatgtcat cttcacgtgc ttgaagtact tctttacgat
     8821 gtggtggacg aagtgcactc atttttctac cgtttgcatc ttcaatttgt ctgatataat
     8881 ccatttcgat tggattaaca aacgttacag cgataccttc tttaccagca cgacccgtac
     8941 gaccaatacg gtgtgtatag ctttcagtat cttgaggtat atcaaagtta taaacatgac
     9001 tcacaccaga aatatctagt cctcttgctg ctacatcagt agcgactaaa atattaattt
     9061 ggtcattttt aaatttcttt aatacttcta aacgtttcgc ttgtgtaata tcaccatgta
     9121 aaccttcagc tttatatcct ttagaaatca aagcacttgt taattcatca acacgacgtt
     9181 ttgtacgtcc gaatacgatt gctaattcag gttgatgaac atctaggaaa tttgtaaatg
     9241 tatcaaattt ctctaattct ttaacaattg tatagaattc ttcgatttgt ggatcagaca
     9301 tttcattatt cattgtctta atgatttttg gtgatttcat aaattgttgt actaaagctt
     9361 ggattgcttt aggcattgta gctgagaaca acattgtttg acgttgtact gctggaattt
     9421 tatccataat aaatctcata tcatcgatga atcccatatt catcatttca tcagcttcat
     9481 ctaaaatcaa agtatgaatt ccgtccgttt ttaatgtgcg acgatttaaa tggtcgataa
     9541 cacgcccagg tgttccgact acgatttgtg ggcctttttt caaggcttta atttggcgtt
     9601 cgataggcat accaccgaat acagtaacaa cttggacacc ttgtccacgg ctaaattctc
     9661 ttaattgttc agctacctgc attgccaatt ctcttgtagg tgctaaaatc aacgattgaa
     9721 ccccttgttt ccctactact ttctcaatta aaggaatacc gaatgctcct gttttacctg
     9781 taccggtttg agcttgccca aggatatcaa ttccttgtaa cgcataaggg atactgtctt
     9841 tttggatagg tgtcggctct ttaaatccca ttgattcaag tgactgaacc gtattatccg
     9901 aaatccctag ttctttaaaa ttttgcaaaa taattctcct ttttacctgt ttatttctca
     9961 atatactttt gcatataagt tcattaatat ttcatcaact tctatatagt accaccaaaa
    10021 aaatttaatc gcaataaagc aatttgcgaa tatttattag aatcacatta attgattgtc
    10081 atatttttgt caaaaagcta gcattagata agatgtaagc gttacgcatc attcgaataa
    10141 tcattataaa aaatttttta taaaatacgt atatcgttaa cattccacaa caaattttac
    10201 tgaaacacat gtgaaattag acattataat actacatctt tttgtaaaac attatataaa
    10261 cattcaaact acttcctata taaagtgtgt acatagaatt aaccttgatt tag
//
LOCUS       DAKTIO010000098        10295 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.098, whole genome shotgun sequence.
ACCESSION   DAKTIO010000098 DAKTIO010000000
VERSION     DAKTIO010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10295
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.098"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..172
                     /locus_tag="O0293_001784"
     CDS             <1..172
                     /locus_tag="O0293_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCY0280668.1"
                     /translation="DNLNSFNKNEIYYIVCAGGVRSAKVVEYLEANGIDAVNVEGGMH
                     AWGDEGLEIKSI"
     gene            498..7058
                     /gene="sasC"
                     /locus_tag="O0293_001785"
     CDS             498..7058
                     /gene="sasC"
                     /locus_tag="O0293_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored repetitive surface protein SasC"
                     /protein_id="HCY0280669.1"
                     /translation="MNLLKKNKYSIRKYKVGIFSTLIGTVLLLSNPNGAQALTTDNNV
                     QSDTNQATPVNSQDKDVANNRGLANSAQNTPNQSATTNQATNQALVNHNNGSIVNQAT
                     PTSVQSSTPSAQNNNHTDGNTTATETVSNANNNDVVSNNTALNVPTKTNENGSGGHLT
                     LKEIQEDVRHSSNKPELVAIAEPASNRPKKRSRRAAPADPNATPADPAAAAVGNGGAP
                     VAITAPYTPTTDPNANNAGQNAPNEVLSFDDNGIRPSTNRSVPTVNVVNNLPGFTLIN
                     GGKVGVFSHAMVRTSMFDSGDNKNYQAQGNVIALGRIHGTDTNDHGDFNGIEKALTVN
                     PNSELIFEFNTMTTKNGQGATNVIIKNADTNDTIAEKTVEGGPTLRLFKVPDNVRNLK
                     IQFVPKNDAITDARGIYQLKDGYKYYSFVDSIGLHSGSHVFVERRTMDPTATNNKEFT
                     VTTSLKNNGNSGASLDTNDFVYQVQLPEGVEYVNNSLTKDFPSNNSGVDVNDMNVTYD
                     AANRVITIKSTGGGTANSPARLMPDKILDLRYKLRVNNVPTPRTVTFNETLTYKTYTQ
                     DFINSAAESHTVSTNPYTIDIIMNKDALQAEVDRRIQQADYTFASLDIFNGLKRRAQT
                     ILDENRNNVPLNKRVSQAYIDSLTNQMQHTLIRSVDAENAVNKKVDQMEDLVNQNDEL
                     TDEEKQAAIQVIEEHKNEIIGNIGDQTTDDGVTRIKDQGIQTLSGDTATPVVKPNAKK
                     AIRDKATKQREIINATPDVTEDEIQDALNQLATDETDAIDNVTNATTNADVETAKNNG
                     INTIGAVVPQVTHKKAARDAINQATATKRQQINSNREATQEEKNAALNELTQATNHAL
                     EQINQATTNADVDNAKGDGLNAINPIAPVTVVKQAARDAVSHDAQQHIAEINANPDAT
                     QEERQAAIDKVNAAVTAANTNILNANTNADVEQVKTNAIQGIQAITPATKVKTDAKNA
                     IDKSAETQHNTIFNNNDATLEEQQAAQQLLDQAVATAKQNINAADTNQEVAQAKDQGT
                     QNIVVIQPATQVKTDTRNVVNDKAREAITNINATTGATREEKQEAINRVNTLKNRALT
                     DIGVTSTTAMVNSIRDDAVNQIGAVQPHVTKKQTATGVLNDLATAKKQEINQNTNATT
                     EEKQVALNQVDQELATAINNINQADTNAEVDQAQQLGTKAINAIQPNIVKKPAALAQI
                     NQHYNAKLAEINATPDATNDEKNAAINTLNQDRQQAIESIKQANTNAEVDQAATVAEN
                     NIDAVQVDVVKKQAARDKITAEVAKRIEAVKQTPNATDEEKQAAVNQINQLKDQAINQ
                     INQNQTNDQVDTTTNQAVNAIDNVEAEVVIKPKAIADIEKAVKEKQQQIDNSLDSTDN
                     EKEVASQALAKEKEKALAAIDQAQTNSQVNQAATNGVSAIKIIQPETKVKPAAREKIN
                     QKANELRAKINQDKEATAEERQVALDKINEFVNQAMTDITNNRTNQQVDDTTSQALDS
                     IALVTPDHIVRAAARDAVKQQYEAKKREIEQAEHATDEEKQVALNQLANNEKRALQNI
                     DQAIANNDVKRVETNGIATLKGVQPHIVIKPEAQQAIKASAENQVESIKDTPHATVDE
                     LDEANQLISDTLKQAQQEIENTNQDAAVTDVRNQTIKAIEQIKPKVRRKRAALDSIEE
                     NNKNQLDAIRNTLDTTQDERDVAIDTLNKIVNTIKNDIAQNKTNAEVDRTETDGNDNI
                     KVILPKVQVKPAARQSVGVKAEAQNALIDQSDLSTEEERLAAKHLVEQALNQAIDQIN
                     HADKTAQVNQDSINAQNIISKIKPATTVKATALQQIQNIATNKINLIKANNEATDEEQ
                     NIAIAQVEKELIKAKQQIASAVTNADVAYLLHDEKNEIREIEPVINRKASAREQLTTL
                     FNDKKQAIEANIQATVEERNSILAQLQNIYDTAIGQIDQDRSNAQVDKTASLNLQTIH
                     DLDVHPIKKPDAEKTINDDLARVTALVQNYRKVSNRNKADALKAITALKLQMDEELKT
                     ARTNADVDAVLKRFNVALSDIEAVITEKENSLLRIDNIAQQTYAKFKAIATPEQLAKV
                     KVLIDQYVADGNRMIDEDATLNDIKQHTQFIVDEILAIKLPAEATKVSPKEIQPAPKV
                     CTPIKKEETHESRKVEKELPNTGSEGMDLPLKEFALITGAALLARRRTKNEKES"
     gene            complement(7175..8443)
                     /locus_tag="O0293_001786"
     CDS             complement(7175..8443)
                     /locus_tag="O0293_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0280670.1"
                     /translation="MYQTIIIGGGPSGLMAAVAASEQSSSVLLIEKKKGLGRKLKISG
                     GGRCNVTNRLPYAEIIKNIPGNGKFLYSPFSIFDNESIIDFFESRGVKLKEEDHGRMF
                     PVSNKAQDVVDTLVTTIERQHVTIKEEEAVSRIEVNTDQTFTVHTQNNSYESHSLVIA
                     TGGTSVPQTGSTGDGYKFAQDLGHTITELFPTEVPITSAEPFIKSNRLKGLSLKDVEL
                     SVLKKNGKKHISHQMDMLFTHFGISGPAALRCSQFVYKEQKNQKTQHISMAIDAFPEL
                     NHEQLKQHITSLLSDTPDKIIKNSLHGLIEERYLLFMLEQAGIDENTTSHHLSNQQLN
                     DLVNMFKGFVFKVNGTLPIDKAFVTGGGVSLKEIQPKTMMSKLVPGLFLCGEVLDIHG
                     YTGGYNITSALVTGHVAGLYAGHYSHASME"
     gene            8850..>10295
                     /locus_tag="O0293_001787"
     CDS             8850..>10295
                     /locus_tag="O0293_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCY0280671.1"
                     /translation="MSESKEMVRGTFLITISILITKVLGVLFIIPFNYLIGGQENMAP
                     FTYAYAPYNIAIAVATAGVPLAASKYVAKYNAIGAYKVSQKFYKSSFIVMSITGVLGF
                     LVLYFLAPYISELTLARNIHDKNGWSVDDITWIIRIISMVVIFIPVLATWRGIFQGYK
                     SMGPTAVSEVTEQIARVIFILIGSYLVLNVFDGSILLANGIATFAAAVGAIIGIFTLW
                     YYWRKRKHNIDRMVESDYTDIDVSYGKMYKEIIAYSIPFVIVSLNYPLFNLVDQFTHN
                     GALSLVGIPSQLQDIFFNMLNMSTNKIVMIPTSLSAGFAVSLIPYITKTFAEGRLHEM
                     HHQIRTSIGVLMFITVPASIGIMALAQPLFTVFYGYDPIVLGHDPNHDGSRLLFYYAP
                     VAILISLLSVTASMLQGIDKQKLTVYVILASVVIKLALNYPLIMLFHTPGAILSTSIA
                     LLFAIGCNFYILKKYAKFKFSYSWIHFAKIFL"
BASE COUNT         3928 a         1663 c         1834 g         2870 t
ORIGIN      
        1 ggataattta aattcattta ataaaaatga aatatattat attgtatgtg ctggtggagt
       61 tcgaagcgct aaagttgtag aatatttaga ggcaaatggc attgatgccg taaatgtcga
      121 aggcggcatg cacgcatggg gcgatgaagg tttggaaata aaaagtattt aaagtagtga
      181 cataatttaa aataatatta catttgtaat gacaccaagt aacgtttcgg ttgcttggtg
      241 ttttttggta tgaattactt tctgttacaa aacaatctaa agcgttcttg ttatgtttta
      301 ttaagatttt aattacaaaa cggaaactaa attgtaataa aataaaactt tattttataa
      361 aatgatgatg ataaaattga gtgaacttaa aatattgtac aaaataatat agctataaat
      421 ataatatgag ggagcgtata tttttagcat aattcttaac aacacagcag agaacagaca
      481 accaggagga aaatgaaatg aatttgttaa agaaaaataa atatagtatt aggaagtata
      541 aagtaggcat attctctact ttaatcggaa cagttttatt actttcaaac ccaaatggtg
      601 cacaagcctt aactacggat aataatgtac aaagcgatac taatcaagca acacctgtaa
      661 attcacaaga taaagatgtt gctaataata gaggtttagc aaatagtgcg cagaatacac
      721 ctaatcaatc tgcaacaacc aatcaagcaa cgaatcaagc attggttaat cataataatg
      781 gtagtatagt aaatcaagct acgccaacat cagtgcaatc aagtacgcct tcagcacaaa
      841 acaataatca tacagatggc aatacaacag caactgagac agtgtcaaac gctaataata
      901 atgatgtagt gtcgaataat accgcattaa atgtaccaac taaaacaaat gaaaatggtt
      961 caggaggaca tctaacttta aaggaaattc aagaagatgt tcgtcattct tcaaataaac
     1021 cagagctagt tgcaattgct gaaccagcat ctaatagacc gaaaaagaga agtagacgtg
     1081 cggcaccggc agatcctaat gcaactccag cagatccagc ggctgcagcg gtaggaaacg
     1141 gtggtgcacc agttgcaatt acagcgccat atacgccaac aactgatcct aatgccaata
     1201 atgcaggaca aaatgcacct aacgaagtgc tgtcatttga tgacaatggt attagaccaa
     1261 gtaccaaccg ttctgtgcca acagtaaacg ttgttaataa cttgccgggc ttcacactaa
     1321 tcaatggtgg caaagtaggg gtgtttagtc atgcaatggt aagaacgagc atgtttgatt
     1381 caggagataa taagaactat caagcacaag gaaatgtaat tgcattaggt cgtatacatg
     1441 gaactgatac gaatgaccat ggcgatttta atggtatcga gaaagcatta acagtaaatc
     1501 cgaattctga attaatcttt gaatttaata caatgactac taaaaacggt caaggcgcaa
     1561 caaatgttat tatcaaaaat gctgatacta atgatacgat tgctgaaaag actgttgaag
     1621 gcggtccaac tttgcgttta tttaaagtac ctgataatgt gagaaatctc aaaattcaat
     1681 ttgtacctaa aaatgacgca ataacagatg cgcgtggcat ttatcaacta aaagatggtt
     1741 acaaatacta tagctttgtt gactctatcg gacttcattc tgggtcacat gtttttgttg
     1801 aaagacgaac aatggatcca acagcaacaa ataataaaga gtttactgta acaacatcat
     1861 taaagaataa tggtaattct ggtgcttctc tagatacaaa tgactttgta tatcaagttc
     1921 aattacctga aggtgttgaa tatgtgaaca attcattgac taaagatttt ccaagtaaca
     1981 attcaggcgt tgatgttaat gatatgaatg ttacatatga tgcagcaaat cgtgtgataa
     2041 caattaaaag tactggagga ggtacagcaa actctccggc acgacttatg cctgataaaa
     2101 tactcgattt aagatataaa ttacgtgtaa ataatgtgcc gacaccaaga acagtaacat
     2161 ttaacgagac attaacgtat aaaacatata cacaagattt cattaattca gctgcagaaa
     2221 gtcatactgt aagtacaaat ccatatacta tcgatatcat catgaataaa gatgcattac
     2281 aagccgaagt tgacagacgt attcaacaag ctgattatac atttgcgtca ttagatatct
     2341 ttaatggtct gaaacgacgc gcacaaacga ttttagatga aaatcgtaac aatgtaccat
     2401 taaataaaag agtttctcaa gcatatattg attcattaac taatcaaatg caacatacgt
     2461 taattcgaag tgttgatgct gaaaatgcag ttaataaaaa agttgaccaa atggaagatt
     2521 tagttaatca aaatgatgaa ttgacagatg aagaaaaaca agcagcaata caagttatcg
     2581 aggaacataa aaatgaaata attggtaata ttggtgacca aacgactgat gatggcgtta
     2641 ctagaatcaa agatcaaggt atacagacct taagtgggga tactgcaaca ccggttgtta
     2701 aaccaaatgc taaaaaagca atacgtgata aagcaacgaa acaaagggaa attatcaatg
     2761 caacaccaga tgttactgaa gacgagattc aagatgcact aaatcaatta gctacggatg
     2821 aaacagatgc tattgataat gttacgaatg ctactacaaa tgctgacgtt gaaacagcta
     2881 aaaataatgg catcaatact attggagcag ttgttcctca agtaactcat aaaaaagctg
     2941 caagagatgc aattaaccaa gcaacagcaa cgaaaagaca acaaataaat agtaatagag
     3001 aagcaactca ggaagagaaa aatgcagcat tgaacgaatt aactcaagca accaaccatg
     3061 ctttagaaca aatcaatcaa gcaacaacaa atgctgatgt tgataacgcc aaaggagatg
     3121 gtctaaatgc cattaatcca attgctcctg taactgttgt taagcaagct gcaagggatg
     3181 ccgtatcaca tgatgcacaa caacatatcg cagagatcaa tgctaatcct gatgcgactc
     3241 aagaagaaag acaagcagca attgacaaag tgaatgctgc tgtaactgca gcaaacacaa
     3301 acattttaaa cgctaatacc aatgctgatg ttgaacaagt aaagacaaat gcgattcaag
     3361 gaatacaagc aattacacca gctacaaaag taaaaacaga tgcaaaaaat gccatcgata
     3421 aaagtgcgga aacgcaacat aatacgatat ttaataataa tgatgcgacg ctcgaagaac
     3481 aacaagcagc acaacaatta cttgatcaag ctgtagccac agcgaagcaa aatattaatg
     3541 cagcagatac gaatcaagaa gttgcacaag caaaagatca gggcacacaa aatatagtag
     3601 tgattcaacc ggcaacacaa gttaaaacgg atactcgcaa tgttgtaaat gataaagcgc
     3661 gagaggcgat aacaaatatc aatgctacaa ctggcgcgac tcgagaagag aaacaagaag
     3721 cgataaatcg tgtcaataca cttaaaaata gagcattaac tgatattggt gtgacgtcta
     3781 ctactgcgat ggtcaatagt attagagacg atgcagtcaa tcaaatcggc gcagttcaac
     3841 cgcatgtaac gaagaaacaa actgctacag gtgtattaaa tgatttagca actgctaaaa
     3901 agcaagaaat taatcaaaac acaaatgcaa caactgaaga aaagcaagtg gctttaaatc
     3961 aagtggatca agagttagca acggcaatta ataatataaa tcaagctgat acaaatgcgg
     4021 aagtagatca agcgcaacaa ttaggtacaa aagcaattaa tgcgattcag ccaaatattg
     4081 ttaaaaaacc tgcagcatta gcacaaatca atcagcatta taatgctaaa ttagctgaaa
     4141 tcaatgctac accagatgca acgaatgatg agaaaaatgc tgcgatcaat actttaaatc
     4201 aagacagaca acaagctatt gaaagtatta aacaagctaa cacaaatgca gaagtagacc
     4261 aagctgcgac agtagcagag aataatatcg atgctgttca agttgatgta gtaaaaaaac
     4321 aagcagcgcg agataaaatc actgctgaag tggcgaagcg tattgaagcg gttaaacaaa
     4381 cacctaatgc aactgacgaa gaaaagcagg ctgctgttaa tcaaatcaat caacttaaag
     4441 atcaagcaat taatcaaatt aatcaaaacc aaacaaatga tcaggtagac acaactacaa
     4501 atcaagcggt aaatgctata gataatgttg aagctgaagt agtaattaaa ccaaaggcaa
     4561 ttgcagatat tgaaaaagct gttaaagaaa agcaacagca aattgataat agtcttgatt
     4621 caacagataa tgagaaagaa gttgcttcac aagcattagc taaagaaaaa gaaaaagcac
     4681 ttgcagctat tgaccaagct caaacgaata gtcaggtgaa tcaagcagca acaaatggtg
     4741 tatcagcgat taaaattatt caacctgaaa caaaagttaa accagctgca cgtgaaaaaa
     4801 tcaatcaaaa agcgaatgaa ttacgtgcta agattaatca ggataaagaa gcaacagcag
     4861 aagaaagaca agtagcacta gataaaatca atgaatttgt aaatcaagcc atgacagata
     4921 ttacgaataa tagaacaaat caacaagttg atgatacaac aagtcaagcg cttgatagca
     4981 ttgctttagt gacgcctgac catattgtta gagcagctgc tagagatgca gttaagcaac
     5041 aatatgaagc taaaaagcgc gaaattgagc aagcggaaca tgcgactgat gaagaaaaac
     5101 aagttgcttt aaatcaatta gcgaataatg aaaaacgtgc attacaaaac atcgatcaag
     5161 caatagcgaa taatgatgtg aaacgtgttg aaacaaatgg cattgctaca ctaaaaggtg
     5221 tacaacctca tattgtaatt aagcctgaag cacaacaagc aataaaagca agtgcagaaa
     5281 atcaagtaga atcaataaaa gatacaccac atgcaacagt tgatgaatta gatgaagcga
     5341 atcaattaat tagcgacaca ctcaaacaag cgcaacaaga aatagaaaat acaaatcaag
     5401 atgctgctgt tactgatgtt agaaatcaaa caatcaaggc aatagagcaa ataaaaccta
     5461 aagtaagacg taaacgagct gcgcttgata gcattgaaga aaataataaa aatcaactcg
     5521 atgcaatccg aaatacgttg gatactactc aagatgaaag agatgttgct attgatactt
     5581 taaataaaat tgtaaataca attaaaaatg acattgcaca aaacaaaacg aatgcagaag
     5641 tggatcgaac tgagactgat ggcaacgaca acatcaaagt gattttacct aaagttcaag
     5701 ttaaaccagc agcgcgtcaa tctgttggtg taaaagccga agctcaaaat gcactaatcg
     5761 atcaaagcga tttatcaact gaagaagaaa gactagctgc taaacattta gtagaacaag
     5821 cacttaatca ggctattgat cagatcaatc atgcagataa gactgcccaa gttaatcaag
     5881 atagtataaa tgctcaaaat attatttcaa aaattaaacc agcgacaaca gttaaagcaa
     5941 cagcattaca acaaattcaa aatatcgcta caaataaaat taatttaatt aaagcaaata
     6001 acgaagcgac agatgaagaa caaaatattg caatagcaca agttgaaaaa gagttaatta
     6061 aagctaaaca acaaattgct agtgcagtga ctaatgcaga tgtggcatat ttattgcatg
     6121 atgagaaaaa cgaaattcgt gaaatcgaac ctgttattaa cagaaaggcg tctgctcgag
     6181 aacaattgac aacattattc aacgataaaa aacaagcaat tgaagcgaat attcaagcaa
     6241 cggtagaaga aagaaatagt atattagcac agttacaaaa tatttatgac actgctattg
     6301 gacaaattga tcaagatcgt agcaatgcac aagttgataa aacagcatca ttaaatctac
     6361 aaacaataca tgatttagat gtacatccta ttaaaaagcc agatgctgaa aaaacgatta
     6421 atgatgatct tgcacgcgtc actgctttag tgcaaaatta tcgaaaagta agtaatcgta
     6481 ataaggctga tgcattaaaa gctataactg ctttaaaatt acaaatggat gaagaattaa
     6541 aaacagcacg cactaatgct gatgttgatg cagttttaaa acgatttaat gttgcattaa
     6601 gcgatataga agcagtaatt actgaaaaag aaaatagctt actgcgaatt gataacattg
     6661 ctcaacaaac atatgcgaaa ttcaaagcga tcgcaacacc agaacaatta gctaaagtaa
     6721 aagtattaat tgatcaatat gttgcagatg gcaatagaat gattgatgaa gatgcgacat
     6781 taaatgacat caaacaacac acgcaattca ttgttgatga aattttagca attaaattac
     6841 cagctgaagc gacgaaagta tcaccaaaag aaattcagcc agctccaaaa gtttgtacgc
     6901 ctattaaaaa agaagagaca catgaatcgc gcaaagttga aaaagaactt ccaaatacag
     6961 gttctgaagg aatggattta ccattgaaag aatttgcact gattacaggt gcggctttgt
     7021 tagctagaag acgtactaaa aacgaaaaag aatcataatt aacgaaatga ccttaatata
     7081 acgacacttt ttagtgactc agttatatta aggtcatttt tatttgaatg aaaaagttga
     7141 tctactaaag agaatcgaac caaattttat actattattc cattgatgca tgtgagtaat
     7201 gtccggcata taatccagcg acatgtcctg ttacgagtgc acttgtaata ttataaccac
     7261 cagtataacc atgtatatct aatacttcac cacataaaaa taatcccgga actaatttag
     7321 acatcattgt tttaggttga atttctttaa gtgacacacc accacctgtg acaaatgcct
     7381 tatctatagg taatgtcccg ttcaccttaa atacaaaccc tttaaacata tttactaagt
     7441 cgttcaattg ttgatttgat aagtgatgtg atgtggtatt ttcatcgatt cctgcttgtt
     7501 ccagcatgaa cagtaagtag cgctcttcaa ttagaccatg caaactgttt ttaatgattt
     7561 tatctggtgt gtccgataat aatgatgtga tgtgttgttt taattgttca tggtttaatt
     7621 caggaaatgc atcgattgcc atagaaatgt gctgtgtctt ttgatttttt tgttctttat
     7681 aaacaaactg actacatctt aatgcagctg gaccactgat accaaaatga gtaaataaca
     7741 tatccatttg atgactgatg tgttttttac catttttctt aagtactgac aattcaacat
     7801 cttttaaact taaacctttt agacgattgg atttgatgaa aggttcagct gatgtaattg
     7861 gaacttcggt cgggaataac tcagtaatgg tatgacctaa atcttgtgcg aacttataac
     7921 catcaccagt tgaaccagtt tgagggacac ttgtaccacc tgtagcaatc actagcgaat
     7981 ggctttcata actattattt tgagtatgta cagtgaaagt ttggtctgta ttaacttcga
     8041 ttctactaac agcttcttct tctttaatcg ttacatgttg gcgttcgata gttgtcacta
     8101 atgtatcaac cacgtcttgt gctttgttgg aaactggaaa catacgcccg tgatcttctt
     8161 cttttaattt aacaccccta gactcaaaaa aatctatgat ggattcatta tcaaaaattg
     8221 aaaagggact atataaaaat ttcccatttc caggaatgtt cttaataatt tcagcatatg
     8281 gtaatcgatt agttacgtta catctaccgc caccagatat tttgagttta cgacctagac
     8341 ctttcttttt ttcaatgagt aacacactgc tactttgttc gcttgcagct actgccgcca
     8401 ttaagccgct aggtccgcct ccgataataa ttgtttgata catatacgct tcctccaaat
     8461 ttgttactgg acattcaatt gctattttat agtacttttt taaactttgc tatatctctt
     8521 gtgttatgag atgacaatta tgttgaatat atttataatg tgaacttcat aaaaaatgaa
     8581 gatgtatgtg tatgataaga atgatttttc tactgttatg cgatgttcat ctcaaatagt
     8641 agtataatgt ttatatgaaa tttcgtaata ttctaatcaa aatctagtat tcaatgacaa
     8701 tgtgttgata attttgttat gataattcat tgaatatacg acaatgaaat aaagttttgt
     8761 gatatattca taataatgaa aaatgatttt acagagaaac agcttttagt ttacaaaact
     8821 ttaataaaca tgagataggg agattcgtaa tgagtgaaag taaagaaatg gtgcgtggaa
     8881 cctttttaat taccattagt atattaatta ccaaggtatt gggcgtactt tttatcattc
     8941 cattcaacta tttaattggt ggacaagaaa atatggcgcc gttcacatac gcttatgcac
     9001 catataatat tgcaattgca gttgcgacag caggtgtacc attagcagct tcgaaatatg
     9061 ttgcgaaata taatgctatt ggggcatata aagtaagtca gaaattttat aaatcgagtt
     9121 ttattgtaat gagtattacc ggtgttttag gtttcttggt actgtatttc ttagcacctt
     9181 atatttctga attaacatta gctagaaaca ttcatgataa aaacggttgg tcagtcgatg
     9241 atattacttg gattataaga atcattagta tggttgttat ctttattcct gtattagcga
     9301 catggagagg gatattccaa ggttataaat ctatggggcc aacagcagtg tcagaagtaa
     9361 ctgagcaaat tgcacgcgtg attttcatat taatcggaag ttatttagta ctaaatgtct
     9421 ttgatggctc tattttatta gccaatggta ttgctacttt tgcagcagca gttggtgcga
     9481 ttattggtat attcacactc tggtattact ggagaaagcg taagcataat attgatcgta
     9541 tggtcgaatc tgattacaca gatatagatg tgtcatatgg caagatgtat aaagaaatta
     9601 ttgcttatag tataccgttc gttattgtaa gtttgaatta tccattattt aacttagtag
     9661 atcaatttac acataacggt gctttatctt tagttggtat accttcacag ttacaagata
     9721 tattctttaa tatgctaaat atgtcaacta ataaaattgt aatgattccg acatcattaa
     9781 gtgctggttt tgcagtgagt ttaattcctt atattacaaa aacatttgca gaaggtcgct
     9841 tacatgaaat gcatcatcaa attagaacat ctattggtgt attaatgttt ataactgttc
     9901 cagctagtat tggaattatg gccttggcac aaccattatt cacagttttc tatggttacg
     9961 atccaattgt tttaggtcat gaccctaacc atgatggtag tcgcttatta ttctattatg
    10021 cgcctgtagc aatactaatt tcactattaa gtgtaacggc atcaatgtta caaggtatag
    10081 ataaacagaa attaacagtt tatgttattt tagcttctgt agttatcaag ttagcgttaa
    10141 actatccatt aattatgtta ttccatacac ctggtgcaat tttaagtact agtatcgcat
    10201 tattatttgc aattggatgt aatttctata ttcttaaaaa atatgcgaag tttaaattca
    10261 gctatagttg gattcatttt gcaaaaatat tcttg
//
LOCUS       DAKTIO010000099        10257 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.099, whole genome shotgun sequence.
ACCESSION   DAKTIO010000099 DAKTIO010000000
VERSION     DAKTIO010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10257
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.099"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(75..146)
                     /locus_tag="O0293_001788"
                     /pseudo
     CDS             complement(75..146)
                     /locus_tag="O0293_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(181..447)
                     /locus_tag="O0293_001789"
     CDS             complement(181..447)
                     /locus_tag="O0293_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCY0280672.1"
                     /translation="MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALE
                     GIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIKIASCKYHY"
     gene            complement(447..698)
                     /locus_tag="O0293_001790"
     CDS             complement(447..698)
                     /locus_tag="O0293_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCY0280673.1"
                     /translation="MIIKNYSYARQNLKALMTKVNDDSVMVTVTSTDDKNVVIMSESD
                     YNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKDIDV"
     gene            complement(863..964)
                     /locus_tag="O0293_001791"
     CDS             complement(863..964)
                     /locus_tag="O0293_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280674.1"
                     /translation="MLRSVSDTNLLGQYEKAISISDMIEIVFLMYEV"
     gene            complement(971..1570)
                     /locus_tag="O0293_001792"
     CDS             complement(971..1570)
                     /locus_tag="O0293_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCY0280675.1"
                     /translation="MTKKLLTLFIVSMLILTACGKKESATTSSKNGKPLVVVYGDYKC
                     PYCKELDEKVMPKLRKNYIDNHKVEYQFVNLAFLGKDSIVGSRASHAVLMYAPKSFLD
                     FQKQLFAAQQDENKEWLTKELLDKHIKQLHLDKETENKIIKDYKTKDSKSWKAAEKDK
                     KIAKDNHIKTTPTAFINGEKVEDPYDYESYEKLLKDKIK"
     gene            complement(1589..1948)
                     /locus_tag="O0293_001793"
     CDS             complement(1589..1948)
                     /locus_tag="O0293_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HCY0280676.1"
                     /translation="MKRFVATVLLLLVFISGCGNDKYVKEIDEAVKIQNQKQEQLAKK
                     GNGDRVDHFERKDANIYVYDKDKIIILAYKPLSNDEVHYYAYDFSDKRVSYKQDFDSR
                     RYYQQHDADYHEENMTN"
     gene            2203..3453
                     /gene="fmhA"
                     /locus_tag="O0293_001794"
     CDS             2203..3453
                     /gene="fmhA"
                     /locus_tag="O0293_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhA"
                     /protein_id="HCY0280677.1"
                     /translation="MNFVTLTSDEFNAFTTKHFSHYTQSAIHYNHRVDLKGDVHLVGV
                     KDDNGQVIAGCLLTEARTLKFFKYFYTHRGPVMDYTNQSLVAFFFKALTSYLKKHNCL
                     YVLVDPYLIENLRNADGEIVKSYDNRAFVRTMDKLGYKHQGFPVGYDSMSQIRWLSVL
                     DLKDKTEDQLLKEMDYQTRRNIKKTYDIGVKTKTLTIDETQTFFDLFHMAEEKHGFKF
                     RELPYFEEMQKLYDDHAMLKLAYIDLNEYLKTLQLKQQQLTAELSGVEEALEESPNSK
                     KNKTKRTQLEQQLNSNKRKIDNTIEQIEQDGAVLNLASALFIYNEHEVYYLSSGSNPK
                     YNAYMGAYHLQWEMIKFAKAHHIDRYNFYGITGDFSESSEDYGVQQFKKGFNAHVEEY
                     IGDFIKPIKPLLYKVQTYLNHKRR"
     gene            complement(3550..4281)
                     /locus_tag="O0293_001795"
     CDS             complement(3550..4281)
                     /locus_tag="O0293_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCY0280678.1"
                     /translation="MIQLNNIHKSFNDVEVIKGIDLSVEQGEVVTLIGRSGSGKTTLL
                     RMINALEIPTEGTVYVNGKTYTSKDKKSQIEVRKQSGMVFQSYNLFPHKTALENVMEG
                     LITVKKLKKDEARGKSLELLEKVGLTHVKDQRPHALSGGQQQRVAIARALAMNPKVML
                     FDEPTSALDPELVNDVLKVIKDLANEGMTMVIVTHEMRFAKEVSNNIVFIHEGMIGEQ
                     GAPEEMFNRPKTEELRRFLNVINEE"
     gene            complement(4278..4997)
                     /locus_tag="O0293_001796"
     CDS             complement(4278..4997)
                     /locus_tag="O0293_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCY0280679.1"
                     /translation="MFLNLNSEQQHALDAAKQAFGPMLEGLVKYSIPITLVTFVLGLI
                     IALFTALMRISTSKILRSISRVYVSIIRGTPMIVQLFIIFYGIPELGRLLTNDADNQW
                     TLAPVVAAIIGLSLNVGAYASEIIRGGIISIPKGQTEAAYSIGMTYGQTIQRIILPQA
                     IRVSIPALGNTFLSLIKDTSLLGFILVAEMFRKAQEVASTTYEYLTIYVLVALMYWVV
                     CFIISIIQGIYESYIERGYRS"
     gene            complement(4978..5757)
                     /locus_tag="O0293_001797"
     CDS             complement(4978..5757)
                     /locus_tag="O0293_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCY0280680.1"
                     /translation="MKRLLFVMIAFVFILAACGNNSSKDKEASKDSKTINVGTEGTYA
                     PFSFHDKDGKLTGYDIDVIKAVAKEEGLKLKFNETSWDSMFAGLDAGRFDVIANQVGI
                     NPDREKKYKFSKPYTFSSAVLVIRENEKDIKDFDDVKGKKLAQTFTSNYGKLAKDKGA
                     DITKVDGFNQSMDLLLSKRVDGTFNDSLSYLDYKKQKPNAKIKAIKGNAEQSRSAFAF
                     SKKADDETVQKFNDGLKKIEENGELAKIGKKWFGQDVSKSK"
     gene            complement(5878..7317)
                     /gene="mdeA"
                     /locus_tag="O0293_001798"
     CDS             complement(5878..7317)
                     /gene="mdeA"
                     /locus_tag="O0293_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdeA"
                     /protein_id="HCY0280681.1"
                     /translation="MSKKQKLTMIITMLMGGFFGLLNETLLVTALPSIMKDFEISYTQ
                     VQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVA
                     RIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYFN
                     WRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISE
                     KGFDNPIVLVSMIGGVVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSM
                     IGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRNLALVGMA
                     IVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSM
                     TNTVQQIAGSIGTAALITILSHASKNFSPTMSDYNGMNKIDMMNQIKVDTMLHGYHAG
                     FLFALLITVVSFFCSFMLQGKKKEVDSRQ"
     gene            7491..7595
                     /locus_tag="O0293_001799"
     CDS             7491..7595
                     /locus_tag="O0293_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCY0280682.1"
                     /translation="MKLDLQTARRNLNSPNIKTRKRALKIIKQHKRAK"
     gene            complement(7749..8435)
                     /locus_tag="O0293_001800"
     CDS             complement(7749..8435)
                     /locus_tag="O0293_001800"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCY0280683.1"
                     /translation="MPKLILCRHGQSEWNAKNLFTGWEDVNLSEQGINEATRAGEKVR
                     ENNIAIDVAFTSLLTRALDTTHYILTESKQQWIPVYKSWRLNERHYGGLQGLNKDDAR
                     KEFGEEQVHIWRRSYDVKPPAETEEQREAYLADRRYNHLDKRMMPYSESLKDTLVRVI
                     PFWTDHISQYLLDGQTVLVSAHGNSIRALIKYLEDVSDEDIINYEIKTGAPLVYELTD
                     DLEVIDKYYL"
     gene            complement(8762..9628)
                     /locus_tag="O0293_001801"
     CDS             complement(8762..9628)
                     /locus_tag="O0293_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCY0280684.1"
                     /translation="MSHNENLKLAQRGAYLSLIVYIILSIVKYVTGFVFNSAAVRADA
                     LNNMTDIIVSLAVIIGLKISIKPADRNHPYGHLKSENISSLLVSFVIMFVGIQVVIQN
                     APRLFKEDDVVPNAITIIVSLISGLVMLIVFAVNQRLAKRTKSSSLNSAAKDNLSDSL
                     VSIGTAIGLIFTQIGFPIVDIILATLLGLLIVYTGFGIFKEAIFMLSDGFNETELEAY
                     RNDILEVDEVQEVKSIKGRYHGSSVFIDVTIVVDANLSLVEAHQICDNVEHHLHKKGI
                     SSVYVHPEPDHL"
     gene            complement(10181..>10257)
                     /locus_tag="O0293_001802"
     CDS             complement(10181..>10257)
                     /locus_tag="O0293_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280685.1"
                     /translation="YVKKRLIQNIQRILSKEVLYGEMQ"
BASE COUNT         3437 a         1888 c         1410 g         3522 t
ORIGIN      
        1 ccacttttcg ttttatctaa tacatgacta ttgtacaaat atgatacaga aaactttata
       61 gtatttacga acgtcataat ataagaaaaa aagacaatct ctatcatatt gctaatagaa
      121 attgcctttt catattgtcc taaaataact taccctattt ttatagtgtg acttttagat
      181 ttaataatgg tatttacatg atgctatttt tatatgattg tcatctactg tataaaccaa
      241 tctatgttcg tgattgatac gtctactata atacccagtc agattcgatt ttaacttttc
      301 aggcttacct ataccttcca atgcaccatt tctgtcaata cttttaagta actcattaat
      361 cttcttcacc atttttttat cgttctgctg aaaatactta taatcttcaa aggcttgagg
      421 cgaaaacgta atatttaacc tagccattat acatctatat ctttcgttat agttttccca
      481 cgttctagat ctgcaattga ttgagctaag tgttcagcat tatttgggtt ctgttggagg
      541 taaagtgttt ccatcatgga gttataatct gattctgaca tgattactac gtttttatca
      601 tcagtagatg ttacagttac cataacacta tcatcattta cttttgtcat aagtgccttt
      661 aaattctgtc gagcgtatga ataattttta ataatcatat ttatcgcctc cttttaacac
      721 acctcaattg tacaaatatc tgtacaataa aacaagcgac acagaaaaaa actactaatc
      781 aagatttcgc acggggaaga atcaatacct atcctgtact gattccctac taaacaatgt
      841 ttacatgata atttccacct atttagactt catacattaa aaagacaatc tctatcatat
      901 cgctaataga aattgccttt tcgtattgtc ctaataaatt ggtatcactt actgatctca
      961 acaattaaga ctatttgatt ttatctttta ataacttctc ataactttca taatcatatg
     1021 gatcttcaac tttctcgcca ttaataaatg cagttggtgt cgtttttata tgattatctt
     1081 tcgctatttt tttatctttc tctgcagctt tccaagactt gctatctttt gtcttgtaat
     1141 cttttataat tttattttcc gtctctttat ctaaatgcag ttgtttaata tgtttatcta
     1201 atagttcttt tgttaaccat tctttatttt catcttgctg ggcagcaaat aattgctttt
     1261 gaaaatctaa aaatgattta ggtgcataca tcaatactgc atgactcgca cgcgaaccaa
     1321 caattgagtc tttacctaag aaagctaaat tgacaaattg gtattccact ttgtgattat
     1381 ctatataatt tttacgcaac tttggcatga ctttttcatc taattcttta caataaggac
     1441 atttatagtc gccatatacg acaactaatg gtttgccgtt tttcgaagat gtcgttgctg
     1501 attctttttt accgcaagct gttaaaatta acatgctcac tataaataat gttagtaatt
     1561 ttttagtcat aataactcct attcatatct agttcgtcat attttcttca tgataatccg
     1621 catcatgttg ttgataatat cgtctcgaat caaaatcttg cttatatgat acacgtttat
     1681 cactaaaatc atatgcataa taatgcactt catcattact caaaggttta taagctaaaa
     1741 tgataatttt atccttatca tagacataaa tattagcatc tttgcgttca aaatgatcaa
     1801 cacgatcacc gttgcctttt ttggcaagtt gttcttgttt ttgattttga attttaactg
     1861 cttcatctat ttctttcaca tatttatcat taccacatcc tgatataaag actagtaata
     1921 ataataccgt cgccacaaat cgcttcaatg ctcccactcc tactatcaac ttccaactaa
     1981 ctttcaatat aattaatact atacaccgtc aaacattaat gcgctacaac tactaccaat
     2041 gcatttaata taaatatcca aagaaataac agttatgtat agtcaaagaa aaatatattt
     2101 cattttctat gtgtatttgt aatcagtttt tgataaaata aaaaaatagc attattaata
     2161 aattcaaaaa ttatagttca attcagagag ggatgggata aaatgaactt tgtaacgttg
     2221 acttcggatg agttcaatgc gtttacaaca aagcattttt cacattacac acaatcagct
     2281 attcattaca atcatagagt tgatttaaaa ggcgatgtgc atcttgtagg ggttaaagat
     2341 gacaatggtc aagtgattgc aggatgctta ttgacagaag cacgcacact taaatttttc
     2401 aaatattttt atacacatcg cgggccagtg atggattata caaatcaatc attagtagca
     2461 tttttcttta aagcattaac gtcatattta aagaaacaca attgtttata tgtccttgta
     2521 gatccatatt taattgaaaa tttacgcaat gcagacggtg aaattgttaa atcttatgat
     2581 aaccgagcat ttgttagaac aatggataaa ttaggttata aacaccaagg tttccctgta
     2641 ggttatgatt caatgagcca aatccgttgg ctgtcagtgt tagatttaaa agataagact
     2701 gaagaccaac ttttaaaaga aatggattat caaacgagac gtaatattaa aaaaacatat
     2761 gatattggtg tcaaaactaa aacgttaacg attgatgaaa cgcaaacttt tttcgactta
     2821 ttccatatgg ctgaggaaaa gcacggtttc aaattccgtg agttaccata ctttgaagaa
     2881 atgcaaaagt tatacgatga ccacgccatg ttaaagttgg cgtatattga tttaaacgag
     2941 tatttaaaaa cgttacaatt aaagcaacaa caattaacag ctgaactctc aggtgttgaa
     3001 gaagcattgg aagaaagtcc taattcaaag aaaaataaaa cgaaacgcac acagctagaa
     3061 caacagctaa atagcaataa gcgtaaaatc gacaatacaa tagaacaaat tgaacaagat
     3121 ggtgcagtct taaacttagc ttctgcttta tttatctaca atgagcatga agtttattac
     3181 ttatctagtg gttcaaaccc taaatataat gcttatatgg gtgcctacca tttgcagtgg
     3241 gaaatgatta agtttgctaa agcgcatcat attgatcgct ataattttta tggtattacc
     3301 ggtgactttt cagagtcatc agaagactat ggcgtccaac aattcaaaaa aggttttaac
     3361 gcacatgtag aagagtatat cggtgacttt attaagccta tcaaaccatt actatataag
     3421 gtacaaacgt acttaaatca taaacgtcgt taaaatatcg ctccaagatt atgacaacat
     3481 aacaaagaac cacgcatcat gtatatggct tcatcgccat actaaaacat gatacgtggt
     3541 tctatttgat tattcttcat ttataacatt taagaaacgt cttaattctt ctgttttcgg
     3601 acgattgaac atctcttctg gagccccttg ttctccgatc atgccttcat gaataaatac
     3661 aatgttatta gatacttctt tagcaaaacg catttcatgt gtcacaatga ccattgtcat
     3721 gccttcatta gccaaatctt taataacctt taaaacatca ttcacaagtt caggatcaag
     3781 tgcagatgtt ggttcatcaa acaacatcac tttagggttc attgctagtg ctcttgcaat
     3841 agcaacacgt tgttgttgac cacctgataa tgcatgtgga cgttgatctt tgacatgtgt
     3901 taaaccaact ttctcaagta actcaagtga tttcccacgt gcctcatcct ttttcaactt
     3961 tttaactgtg ataagacctt ccattacatt ttctaatgcc gtcttatgcg gaaaaaggtt
     4021 ataactttga aataccatac cagactgttt acgaacttct atttgtgatt ttttatcttt
     4081 agatgtatat gttttgccgt taacataaac tgtaccttca gttggaattt ctaatgcatt
     4141 aatcatacgt aacaatgttg ttttacctga accagatcga ccgattaagg ttacaacctc
     4201 accttgttca acagataaat caataccttt gatgacttca acatcattaa atgatttatg
     4261 gatattgttc aattgaatca tgagcgatac cctctttcaa tataagattc atagatacct
     4321 tggataattg aaataataaa gcataccacc cagtacatta gcgcaactaa cacataaatt
     4381 gttaaatatt catatgttgt agacgcaact tcttgagctt ttctaaacat ttcagccact
     4441 aaaataaatc ctaataatga tgtatctttg attaaactta aaaatgtatt acctagtgca
     4501 ggaatcgaca ctcgaattgc ctgcggtaaa atgatacgtt gtatcgtttg accatacgtc
     4561 ataccgatgg aatatgcagc ttctgtttgt cctttcggta tagaaataat accgccacga
     4621 ataatttccg aagcatacgc acctacattt aatgataaac caataatagc agccactaca
     4681 ggtgccaatg tccattggtt gtcagcgtca tttgttaata atctacctaa ttctggaata
     4741 ccataaaata tgataaatag ttgtactatc attggtgttc ctcgaataat agatacatag
     4801 acacgtgaaa tacttcttaa aattttactc gttgaaattc gcattaatgc tgtaaataat
     4861 gcaataatca atcctaaaac aaatgtaact aatgtaatag gaattgaata tttgaccaaa
     4921 ccttctagca taggtccgaa agcttgtttt gcagcatcta gcgcatgttg ttgttcgcta
     4981 tttagattta gaaacatctt gaccaaacca tttcttacct attttagcta attcaccgtt
     5041 ttcctcgatt tttttcaagc catcattgaa tttttgaact gtttcatcat ctgctttttt
     5101 agaaaatgca aatgcagatc tactttgttc agcattacct ttgattgctt tgatcttagc
     5161 attaggtttt tgttttttat aatccaagta tgacagacta tcattaaatg taccatcaac
     5221 acgcttagac aataataaat ccattgattg gttaaagcca tcaacttttg taatatcagc
     5281 acctttatcc ttagctaatt taccataatt agatgtgaat gtttgtgcta acttcttacc
     5341 tttaacatca tcaaaatctt taatatcttt ttcattttca cgaataacta aaacagcact
     5401 tgagaatgtg taaggcttag aaaatttata tttcttttct ctatcaggat taatacctac
     5461 ttggttcgcg attacatcaa aacgccctgc gtctaaacct gcaaacatag aatcccaaga
     5521 agtttcatta aatttaagtt ttaaaccttc ttctttagcc actgctttaa taacatcaat
     5581 atcgtaacca gttaatttac catctttatc gtggaaacta aatggtgcat aagtcccctc
     5641 agtcccaaca ttaattgtct tgctatcttt actagcttcc ttgtctttcg acgaattgtt
     5701 tccgcatgca gccaatatga aaacgaaagc tatcatcaca aataaaagtc ttttcataga
     5761 atatatctcc ttattcttat tattctaatc ggaataaaaa tattttaatc tgaaatgccg
     5821 gataagtcaa tatgttgaac ttttgctaaa aagctttata tagctagcaa cttatattta
     5881 ttgacgagaa tcaacttctt ttttcttacc ttgtaacata aatgaacaaa agaaactaac
     5941 aactgtaatc aataatgcaa ataaaaatcc agcgtgataa ccatgcaaca tcgtatcaac
     6001 tttaatttga ttcatcatat caattttgtt catgccatta tagtctgaca tagttggaga
     6061 aaagtttttg cttgcatggg ataaaatagt aattaatgca gctgttccga tagaaccagc
     6121 aatttgttgt actgtgtttg tcatagatga accatgtgca ttcatttctg gctttaattg
     6181 attcatcgta tgggtcatta ctggaattaa tcctaacgcg ataccaacca ttcgaatcgc
     6241 ataaactgtt gccaacatga ttgttgatgt ttgttcatcc attacaacaa aataagccgt
     6301 agtaataaca acaatcgcca tacctactaa agcaagattt ctaggaccaa acttttcata
     6361 taaagcaccc gaagtcatcg acataaatgc cataacaatt gctcctggta ataaagttaa
     6421 tccagaatct aaagctgaac gatgcaataa attttgcaca aagataggta aaatcgtttc
     6481 cgaaccaatc atcgatacca ttgtgacacc cataataatg ataccaactg taaattgttt
     6541 atttttaaat acagcaaaat ttaataatgg tgttgatagc cgatattgac gtaatacaaa
     6601 taatgcgact aaaacaacgc ctccaatcat agatactaat acgataggat tatcaaaacc
     6661 tttttctgaa attgaactcg ttccatacaa taacccgccg aaacctagta cagaaaaaat
     6721 gacagaaata aaatcaatag gtactttaat tgtagttcca acatttttta tcgttttaaa
     6781 tccaattaaa aatgtcacag ctgcaattgg agcgacaaca tggaaaagcg atctccagtt
     6841 aaaatattca acaaggtaac ctgaaagagt aggtccaata gctggtgcta aaccaattac
     6901 caaaccaaat atccccatat atttaccgcg ttcatgtggt tggaaaacat ccaaaatcgt
     6961 tgtcatcatc aatggcatca taatacctgc gcctaacgcc tgaataattc tagcaactaa
     7021 taatgttgca aagtgcggac tcaagccgcc gagtaatgta cctaagaaaa agatagaaat
     7081 acccactaaa aacacttgtc ttgttgtata acgttgtata acaagcgcgg acaaaggaat
     7141 aacaatccca ttagtcaata aaaaagctgt tgtcagccat tgaacttgtg tatatgaaat
     7201 ttcaaaatct ttcataatac ttggtaaagc cgtcactaat agtgtttcat ttaataatcc
     7261 aaaaaatcca cccatcagca tagtaataat catcgttaat ttttgtttct tcgacattta
     7321 aagcttcccc ctcaccatct aaaaaacgac acacattttt agatatccgg taattatacc
     7381 cattttccaa aaaattagca cgactttatt acaatttctt taaaacgtaa tcattactat
     7441 ttacatatga gtagttatcg gtcataatat atttacaagg agggatagag atgaaattag
     7501 atttacagac tgctcgtcgt aatttaaata gccctaacat taaaacaaga aagcgtgctt
     7561 taaagattat taagcaacat aaaagagcga aataattgtt ttaatttgac tatattgtat
     7621 gtctaactat attttcgagt atccctagct cgttaatata aactctatac ttaactattt
     7681 ccttacatta cttcacatat ttaagagctg catatacact cactccttgt gtacatgcag
     7741 ctcttttttt ataagtagta tttatctata acttctaaat catccgttaa ttcataaaca
     7801 agcggtgcac ctgttttaat ttcataatta atgatatctt catctgacac atcttcaaga
     7861 tatttaatca atgcgcgaat tgaatttccg tgtgcagaaa ctaataccgt ttgaccatct
     7921 agcaaatatt gtgaaatatg atctgtccaa aatggtatca ctcgaactaa agtatctttc
     7981 agactttcag aataaggcat catacgttta tctaaatgat tatatcgacg atcagctaag
     8041 taagcttcac gttgttcttc ggtttcagca ggtggtttca catcataaga acgacgccaa
     8101 atatgtactt gttcttctcc aaattctttt ctagcatcat ctttatttaa gccttgcaat
     8161 ccaccatagt ggcgttcatt taaacgccag cttttatata caggaatcca ttgttgttta
     8221 gattcagtta aaatataatg cgttgtatct aaagcacgtg ttaataacga tgtaaaagct
     8281 acatcgatgg caatgttatt ttctcttact ttttcacctg ctctagtcgc ttcattaata
     8341 ccttgttcag ataaattaac atcttcccat ccagtaaata agtttttagc attccactcg
     8401 ctttgtccat gacgacataa aattaatttt ggcataactt ataacctctt tcctttaatc
     8461 tttaggacgt tatttcaggg atattttaag atgatttaat cttagtaatt ttcttttcaa
     8521 tatattttaa aattcaatga ttaaatcaat tatcactaga cattaaatac ataaatccta
     8581 ttccccattt tcatttttta attcataaat gaatcaataa ccacctaata acaaatcata
     8641 ttatacacct ttgttcgcta tttttctaag gtttaaaaaa tatttttagg taaacctaaa
     8701 aatagatgta ataaaaacgc ctcctcagat atttatatat ctatgaagac gtttaaatac
     8761 attatagatg gtctggttct gggtgaacgt atactgagga aatacctttt ttgtgcaaat
     8821 gatgttcgac attgtcacaa atttgatgcg cttctactaa ggaaaggtta gcatctacaa
     8881 caattgtgac atcaataaac acactacttc catggtaacg ccctttaata cttttaactt
     8941 cttgtacttc atcaacttct aaaatatcat tgcgatacgc ttctaattca gtttcattga
     9001 aaccatcact caacataaaa attgcttctt taaaaatacc aaaaccagta taaacgatta
     9061 gtaagcctag taatgttgct aaaataatat cgacaattgg gaaaccgatt tgcgtaaaaa
     9121 ttaatcctat cgctgttcca atgctgacta aactatccga taaattatct ttggcagccg
     9181 aatttaaaga actacttttc gttcttttcg ctagtctttg attgactgca aatacaatca
     9241 acattacaag accactgatt aagctgacga taattgttat tgcgttaggt acaacgtcat
     9301 cttctttgaa caaacgaggt gcattttgaa taactacttg gatacctaca aacataatga
     9361 caaatgacac caataatgaa gaaatatttt cagacttcaa atggccataa ggatgatttc
     9421 gatcggcagg tttaattgaa attttcaatc caataataac agctaaagaa acgataatat
     9481 ctgtcatatt gtttaatgca tcggctctta cagctgcaga gttaaagaca aaacccgtga
     9541 catacttaac aatagataag attatatata caattaaact caaataagca ccgcgttgcg
     9601 ccaatttaag attttcatta tgagacatgc gttgaaccac cttgaattag tatagtaaca
     9661 atattatgaa tgattcattt taattttaca acgtttttaa tttttataaa tttttataaa
     9721 attaaactaa tttattcatt cgcaacccct aaaaataatt tttagccttt ctgcgaattt
     9781 tatgagctag aaaggcgccc aactctccct gtttgttaac tttcgcctcg aaagtttcta
     9841 tgttgggacc ctatgcatta gtagcgaagc tatctaatgt gttcctttat tttctgcgaa
     9901 ttttatgagc tagaaaggct tatgcagttg acgttttacg tccaactgcg ttcctccgtc
     9961 ttcttcaaat ttatttgtta gaaaggcacc caactctccc tgtttgttaa ctttcgcctc
    10021 gaaagtttct atgttagaac cctatgcatg agttgcgaag ctatctaatg tcgtgaacta
    10081 attatataga agaaaaagtg catcaatgac aaattaaatg agatttctac tctaccaaac
    10141 tctcttcgaa agacaatttt ctcctctatt tattagcaaa ctattgcatt tctccatata
    10201 gtacttcctt acttaaaata cgctgaatgt tctgaattaa acgctttttt acatagt
//
LOCUS       DAKTIO010000100        10151 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.100, whole genome shotgun sequence.
ACCESSION   DAKTIO010000100 DAKTIO010000000
VERSION     DAKTIO010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10151
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.100"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(60..3233)
                     /gene="carB"
                     /locus_tag="O0293_001803"
     CDS             complement(60..3233)
                     /gene="carB"
                     /locus_tag="O0293_001803"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCY0280686.1"
                     /translation="MPKRNDIKTILVIGSGPIIIGQAAEFDYAGTQACLALKEEGYRV
                     ILVNSNPATIMTDKEIADKVYIEPLTHDFIARIIRKEQPDALLPTLGGQTGLNMAIQL
                     HESGVLQDNNVQLLGTELTSIQQAEDREMFRTLMNDLNVPVPESDIVNTVEQAFKFKE
                     QVGYPLIVRPAFTMGGTGGGICHNDEELHEIVSNGLHYSPATQCLLEKSIAGFKEIEY
                     EVMRDKNDNAIVVCNMENIDPVGIHTGDSIVVAPSQTLSDVEYQMLRDVSLKVIRALG
                     IEGGCNVQLALDPHSFDYYIIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     LNPITGTSYAAFEPTLDYVISKIPRFPFDKFEKGERELGTQMKATGEVMAIGRTYEES
                     LLKAIRSLEYGVHHLGLPNGESFDLDYIKERISHQDDERLFFIGEAIRRGTTLEEIHN
                     MTQIDYFFLHKFQNIIDIEHQLKEHQGDLEYLKYAKDYGFSDKTIAHRFNMTEEEVYQ
                     LRMENDIKPVYKMVDTCAAEFESSTPYYYGTYETENESIVTDKEKILVLGSGPIRIGQ
                     GVEFDYATVHAVWAIQKAGYEAIIVNNNPETVSTDFSISDKLYFEPLTEEDVMNIINL
                     EKPKGVVVQFGGQTAINLADKLAKHGVKILGTSLENLNRAEDRKEFEALLRKINVPQP
                     QGKTATSPEEALANAAEIGYPVVVRPSYVLGGRAMEIVDNDKELENYMTQAVKASPEH
                     PVLVDRYLTGKEIEVDAICDGETVIIPGIMEHIERAGVHSGDSIAVYPPQTLTEDELA
                     TLEDYTIKLAKGLNIIGLINIQFVIAHDGVYVLEVNPRSSRTVPFLSKITDIPMAQLA
                     MRAIIGEKLTDMGYQEGVQPYAEGVFVKAPVFSFNKLKNVDITLGPEMKSTGEVMGKD
                     TTLEKALFKGLTGSGVEVKDHGTVLMTVSDKDKEEVVKLAQRLNEVGYKILATSGTAN
                     KLAEYDIPAEVVGKIGGENDLLTRIQNGDVQIVINTMTKGKEVERDGFQIRRTTVENG
                     IPCLTSLDTANALTNVIESMTFTMRQM"
     gene            complement(3226..4326)
                     /gene="carA"
                     /locus_tag="O0293_001804"
     CDS             complement(3226..4326)
                     /gene="carA"
                     /locus_tag="O0293_001804"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCY0280687.1"
                     /translation="MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETIS
                     DPSYTGQIITFTYPLIGNYGINRDDFESLVPTLNGIVVKEASAHPSNFRQQKTLHDVL
                     ELHQIPGIAGVDTRSITRKIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVS
                     TKTPYVSTGKDLSVVLVDFGKKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVML
                     SNGPGNPEVVECAIPMIQGILGKIPFFGICLGHQLFALSQGASSFKMKFGHRGANHPV
                     KNLETGKVDITSQNHGYAIDIDSLKSTDLEVTHLALNDGTVEGLKHKTLPAFSVQYHP
                     EANPGPSDSNYLFDDFVAMMTNFKEKERHINA"
     gene            complement(4328..5602)
                     /locus_tag="O0293_001805"
     CDS             complement(4328..5602)
                     /locus_tag="O0293_001805"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCY0280688.1"
                     /translation="MKLIKNGKVLQNGELQQADILIDGKVIKQIAPAIEPSNGVDIID
                     AKGHFVSPGFVDVHVHLREPGGEYKETIETGTKAAARGGFTTVCPMPNTRPVPDSVEH
                     FEALQKLIDDNAQVRVLPYASITTRQLGKELVDFPALVKEGAFAFTDDGVGVQTASMM
                     YEGMIEAAKVNKAIVAHCEDNSLIYGGAMHEGKRSKELGIPGIPNICESVQIARDVLL
                     AEAAGCHYHVCHVSTKESVRVIRDAKRAGIHVTAEVTPHHLLLTEDDIPGNNAIYKMN
                     PPLRSTEDREALLEGLLDGTIDCIATDHAPHARDEKAQPMEKAPFGIVGSETAFPLLY
                     THFVKNGDWTLQQLVDYLTIKPCETFNLEYGTLKENGYADLTIIDLDSEQEIKGEDFL
                     SKADNTPFIGYKVYGNPILTMVEGEVKFEGDK"
     gene            complement(5620..6501)
                     /locus_tag="O0293_001806"
     CDS             complement(5620..6501)
                     /locus_tag="O0293_001806"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HCY0280689.1"
                     /translation="MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLF
                     FENSTRTKCSFEMAELKLGLKTISFETSTSSVSKGESLYDTCKTLESIGCDLLVIRHP
                     FNNYYEKLANINIPIANAGDGSGQHPTQSLLDLMTIYEEYGYFEGLNVLICGDIKNSR
                     VARSNYHSLKALGANVMFNSPNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGL
                     AEETRFAADDYHQKHGLNEVRYNKLQEHAIVMHPAPVNRGVEIQSDLVEASKSRIFKQ
                     MENGVYLRMAVIDELLK"
     gene            complement(6529..7836)
                     /locus_tag="O0293_001807"
     CDS             complement(6529..7836)
                     /locus_tag="O0293_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCY0280690.1"
                     /translation="MQNDEMFERTVKPVLDVNEKPQPAQWAFLSLQHLFAMFGATVLV
                     PFLTGLPISAALLASGIGTLLYILITKAQIPAYLGSSFAFITPIITGLSTHSLGDMLV
                     ALFMSGVMYVIIGILIKLSGTAWLMKLLPPVVVGPVIMVIGLSLAPTAVNMAMYENPG
                     DMKGYNISFLIVAMITLLVTIVVQGFFKGFLSLIPVLVGIIVGYVVAIFMGIVKFDAI
                     MSAKWIDFPHIYLPFKDYVPSFHLGLVLVMIPIVFVTVSEHIGHQMVLNKIVGRNFFE
                     KPGLDKSIIGDGVSTMFASIIGGPPSTTYGENIGVLAITRIYSIYVIGGAAVIAIVLA
                     FIGKFTALISSIPTPVMGGVSILLFGIIAASGLRMLVESKVDFANNRNLVIASVILVV
                     GIGNLVFNLKEIGINLQIEGMALAALSGIILNLILPKEKKQNN"
     gene            complement(8054..8581)
                     /gene="pyrR"
                     /locus_tag="O0293_001808"
     CDS             complement(8054..8581)
                     /gene="pyrR"
                     /locus_tag="O0293_001808"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HCY0280691.1"
                     /translation="MSERIIMDDAAIQRTVTRIAHEILEYNKGTDNLILLGIKTRGEY
                     LANRIQDKIHQIEQQRIPTGTIDITYFRDDIEHMSSLTTKDAIDIDTDITDKVVIIID
                     DVLYTGRTVRASLDAILLNARPIKIGLAALVDRGHRELPIRADFVGKNIPTSKEETVS
                     VYLEEMDQRNAVIIK"
     gene            complement(8980..9897)
                     /locus_tag="O0293_001809"
     CDS             complement(8980..9897)
                     /locus_tag="O0293_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCY0280692.1"
                     /translation="METYEFNITDKEQTGMRVDKLLPELNNDWSRNQIQDWIKAGLVV
                     ANDKVVKSNYKVKLNDHIVVTEKEVVEADILPENLNLDIYYEDDDVAVVYKPKGMVVH
                     PSPGHYTNTLVNGLMYQIKNLSGINGEIRPGIVHRIDMDTSGLLMVAKNDIAHRGLVE
                     QLMDKSVKRKYIALVHGNIPHDYGTIDAPIGRNKNDRQSMAVVDDGKEAVTHFNVLEH
                     FKDYTLVECQLETGRTHQIRVHMKYIGFPLVGDPKYGPKKTLDIGGQALHAGLIGFEH
                     PVTSEYIERHAELPQDFEDLLDTIRKRDA"
     gene            complement(9897..>10151)
                     /gene="lspA"
                     /locus_tag="O0293_001810"
     CDS             complement(9897..>10151)
                     /gene="lspA"
                     /locus_tag="O0293_001810"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCY0280693.1"
                     /translation="YFFIKDAQYNLFMQVAISLLFAGALGNFIDRILTGEVVDFIDTN
                     IFGYDFPIFNIADSSLTIGVILIIIALLKDTSNKKEKEVK"
BASE COUNT         3083 a         1996 c         1495 g         3577 t
ORIGIN      
        1 aatataaact tctttaagta aatggttaat tacagcccca ccgatacagt ttgattgatt
       61 tacatttgac gcattgtaaa tgtcatactt tcaattacat tcgttaaggc attagctgta
      121 tctaaagatg tcaaacatgg aataccattt tcaactgtag tacgtctaat ttggaagcca
      181 tccctttcta cttctttacc tttagtcatt gtatttataa cgatttgaac atcaccattt
      241 tgaatacgtg ttaataaatc attttcgcca ccaattttgc ctactacttc tgcaggtatg
      301 tcatactcag ctaatttatt agctgttcca gacgttgcta aaattttata gccaacttca
      361 tttaagcgtt gtgccaattt aacaacttcc tctttatctt tgtcactgac ggtcattaat
      421 actgtaccgt gatctttaac ttcaacgcca ctacctgtta accctttgaa taacgccttt
      481 tctaatgtag tatctttccc catcacttca cctgttgact tcatttcagg tcctaaagta
      541 atatcaacat ttttcaattt attaaaacta aatactggtg ctttcacaaa gacaccctca
      601 gcatatggtt gaaccccttc ttgataaccc atatctgtta gtttttcccc aatgattgct
      661 cgcatagcta attgtgccat tggaatatca gtaattttac ttaagaatgg taccgttcta
      721 ctagaacgtg gatttacttc taaaacatac acaccatcgt gagctataac gaattgaatg
      781 ttgattaagc caatgatgtt taaaccttta gctaatttta tagtatagtc ctcaagtgtt
      841 gctaactcgt cttctgtcaa agtttgtggt ggatatacag cgattgagtc accactatgc
      901 acaccagcac gttcaatatg ttccatgatt cctggaataa tgaccgtttc tccatcacaa
      961 atcgcatcaa cttcaatttc tttaccagtt aaatatctat cgactagtac cggatgttcc
     1021 ggactcgctt ttacagcctg ggtcatatag ttttctaact ctttgtcatt gtctacaatt
     1081 tccattgcgc gaccacctaa tacataagaa ggtcttacta caaccggata tccgatttct
     1141 gcagcattcg ctaatgcttc ctcaggtgat gtagctgttt tcccttgtgg ctgtggcacg
     1201 ttaatttttc ttaatagtgc ttcaaattct tttctatctt cagcacgatt tagattttct
     1261 agtgaagtac caagtatttt aacaccatgt ttagccaatt tgtctgctaa attaatcgct
     1321 gtttgtcctc caaattgtac aacgacacct ttaggttttt ctaaattaat gatattcatc
     1381 acatcttctt cagttaaagg ttcaaagtat aatttgtcag aaattgagaa gtctgttgaa
     1441 actgtttctg gattgttatt cacaattatc gcttcgtacc ctgctttttg aattgcccaa
     1501 acggcgtgaa ctgtcgcata gtcaaattct acaccttggc cgattcgaat tggtccagag
     1561 cctaatacta agattttttc tttgtcagta actatggatt cattttcagt ttcgtatgta
     1621 ccataataat atggtgttga agattcaaat tcagctgcgc aagtatcaac catcttgtaa
     1681 acaggtttaa tatcattttc catacgcaat tgatatactt cttcttccgt catattaaag
     1741 cgatgcgcta ttgttttatc actaaatcca taatcttttg catatttaag atattctaaa
     1801 tcaccttgat gctcttttaa ttgatgctca atatcaataa tgttttggaa cttgtgtaag
     1861 aagaagtaat caatctgagt catattatga atttcttcta atgttgtgcc tcttctaatt
     1921 gcttcgccga tgaaaaataa tcgttcatca tcttggtgtg aaatacgttc tttaatataa
     1981 tcaagatcga agctttcacc atttggtaat cctaagtgat gcacaccata ctcaagtgat
     2041 cgaattgctt ttaacaatga ttcttcgtaa gttcgaccaa tggccataac ttcacctgtt
     2101 gctttcattt gtgtgccaag ctctcgttct cctttttcaa atttatcaaa aggaaatctt
     2161 ggtatttttg aaatcacata gtctaaagtt ggttcaaacg ctgcataaga tgttcctgta
     2221 attggattta acatttcatc taatgttaga ccaaccgcga ttttagcagc taattttgca
     2281 ataggatatc ctgttgcttt tgaagctaac gctgatgaac gtgatacacg cggatttact
     2341 tctataatat aataatcgaa tgaatgggga tctaatgcta attgaacatt acaaccacct
     2401 tcgataccta aagctcgaat aacttttaat gaaacatcac gtaacatttg atactcaaca
     2461 tctgataatg tttgactagg agccacaaca attgaatcgc ctgtatgaat accaactgga
     2521 tcaatatttt ccatgttaca tacaacgatg gcattatcgt ttttatcacg cattacttcg
     2581 tattcgattt ctttaaaacc tgcgatagat ttttctaata aacattgcgt tgctggacta
     2641 taatgaagac catttgagac gatttcatgt aattcttcat cattatgaca aataccgcct
     2701 ccggtaccac ccatcgtaaa tgccggtcta acaattagcg ggtatcccac ttgctctttg
     2761 aatttaaagg cttgctctac tgtatttaca atgtcactct ctggtacagg aacgtttaaa
     2821 tcattcatta atgttctaaa catttcacgg tcttctgctt gttgaattga tgttagctca
     2881 gttcctaata attggacgtt attatcttga agcacaccac tttcgtgtag ttgaatcgcc
     2941 atgtttaaac ctgtttgacc acctaaagtt ggaagtaaag cgtcaggttg ctctttacgt
     3001 ataattcgcg ctataaaatc atgagttaac ggttcgatat atactttatc cgcaatttcc
     3061 ttatcagtca tgattgtcgc tggatttgaa tttacaagaa taactcgata tccctcttct
     3121 tttaaagcta gacatgcttg tgttccagca taatcaaatt cagctgcttg acctatgata
     3181 attggcccag accctattac taaaattgtt ttgatatcat tacgtttagg cattgatatg
     3241 acgctccttt tccttaaaat tagtcatcat tgctacaaaa tcatcaaata gatagtttga
     3301 atctgacggt cctggatttg cttcaggatg gtattgaaca gaaaatgctg gtaatgtttt
     3361 atgttttaaa ccttctacag taccatcatt taatgcaaga tgagtaactt ctaaatcagt
     3421 actttttaac gaatctatat ctattgcata tccatggttt tgactcgtaa tatcaacttt
     3481 tccagtctct aaatttttaa ctggatggtt cgcaccacga tgaccaaact tcattttaaa
     3541 tgagcttgct ccttgagata atgcaaaaag ttgatgtcct agacagatac caaagaacgg
     3601 aattttccct aaaattcctt gaatcattgg aatcgcacat tctacaactt caggattacc
     3661 tggtccgttt gatagcataa cgccatctgg agccattgct aaaatttctt cggcagtagt
     3721 tgtatatggt acaactgtga cgttacaacc tctgacgttt aattctcgaa caatattttg
     3781 cttcttacca aagtctacaa gtacgacact tagatcctta cctgtcgaaa catacggtgt
     3841 tttagtcgaa acgatttcta cttcgttttt aggtaattct acttgttgta aatgtttgac
     3901 aagttgatca atatcttctt ttcgatcagt aaaaccagct tttaacacac cgtgttgtcg
     3961 aattttacgc gtaatacttc ttgtatcaac acctgcaatc cctggaattt gatgcaattc
     4021 taaaacgtca tgaagtgtct tttgctgtct aaaattactt ggatgcgcac tcgcttcttt
     4081 cactacaata ccgtttaatg taggtactaa tgattcaaaa tcgtctctat tgataccata
     4141 attaccgatt aatggatacg taaaagtaat gatctgacct gtatatgatg gatctgaaat
     4201 agtttcttga taacctgtca tcgctgtatt aaatacaatt tctcctacag ttaagttatc
     4261 agatcctaaa cggtagcctt cgtaaaaaga accgtcttct aacactagat aacgtttgct
     4321 ttgcatatta tttatccccc tcaaatttaa cttcgccttc aaccattgtt aagatcggat
     4381 ttccataaac tttatagccg ataaatggtg tattatctgc ttttgataag aaatcttctc
     4441 ctttaatttc ttgttcacta tctaaatcaa tgattgttaa atctgcataa ccattttctt
     4501 ttaatgtgcc gtattctaaa ttaaatgtct cacatggttt aattgttaag taatctacta
     4561 attgttgtaa tgtccaatca ccatttttta caaaatgcgt atataataat gggaatgctg
     4621 tttcactacc aacaattccg aatggtgctt tttccattgg ttgtgctttt tcatcacgtg
     4681 catgtggtgc atggtctgtt gcgatacagt caattgtacc gtctagtaac ccttctaaca
     4741 aagcctctct atcttcagta cttctcaatg gtggattcat tttataaatg gcattattac
     4801 caggaatatc atcttctgtt aacaataaat ggtgtggtgt aacttcagct gtaacatgaa
     4861 tgcctgcgcg tttagcgtca cgaatgactc taacactttc tttagtagaa acatgacata
     4921 catgataatg acaacctgct gcttcagcta atagtacatc tcttgcgatt tgaacagatt
     4981 cacaaatgtt tggaatacct ggtataccta actctttact gcgtttccct tcatgcattg
     5041 caccaccata gattaatgaa ttatcttcac agtgtgctac gatggctttg tttacttttg
     5101 cagcttcaat catgccttca tacatcatgc ttgcagtttg tactcctaca ccgtcatctg
     5161 taaacgcaaa ggcaccttct tttactagtg ctgggaaatc aaccaattct ttacctaatt
     5221 gacgtgttgt aattgaagca taaggtaata cacgtacttg agcattgtca tcgattaatt
     5281 tttgtaaagc ttcaaaatgt tctacagaat ctggtaccgg tcttgtgtta ggcattggac
     5341 atacagttgt aaatccgcct ctagcagcag ctttagtacc agtttcaatt gtctctttat
     5401 attcaccacc aggttcacgt aaatgaacat gaacatcgac aaatccaggt gacacaaagt
     5461 gacctttcgc atctatgatg tcaacaccat tgcttggttc aattgcaggt gcaatttgtt
     5521 taattacctt accatcaatt aaaatatctg cttgttgtaa ttcgccattt tgtaatactt
     5581 taccgttttt aattaatttc atcattttcg tccccttacc tattttaata attcatcaat
     5641 gactgccatt cttaagtaaa cgccattttc catttgctta aaaattcttg attttgaagc
     5701 ttctactaaa tcgctttgta tttctactcc tctattcaca ggtgccggat gcataacaat
     5761 agcatgttct tgtaatttgt tatagcgtac ttcatttaag ccatgctttt gatgataatc
     5821 atctgctgca aatctagttt cttctgcaag cccatgcctt tcatgttgaa ttcttaataa
     5881 cataactata tctactgttt ctataacatc atctatattt acataaggtg cttctaaaga
     5941 atcatcaatc caagcatttg ggctattaaa cattacgttt gcacctaatg cttttaaact
     6001 atggtaatta ctacgtgcga cacgtgaatt tttaatgtct ccacaaatca atacattcaa
     6061 gccttcaaaa tatccatatt cttcatatat cgtcattaaa tcaagtaaac tttgtgttgg
     6121 atgttgtcca ctaccatcac cagcattcgc aattgggatg ttaatattcg ctaatttttc
     6181 atagtagtta ttaaacggat gtctaatgac taataaatca cagccaatac tttctaaagt
     6241 tttacatgtg tcatataaag attcaccttt tgaaacagat gatgttgatg tttcaaagct
     6301 aatcgttttt aaccctagct taagttctgc catttcaaaa ctacattttg ttcgagtaga
     6361 attttcaaag aataaatttg cgacatattt cccttcaaag tttggtagtt gacgttcacc
     6421 agatttaaat tgacttgcct tttggataag tttgtatatt tgatctgtag ataaatgttc
     6481 cattgataat aaatgattca taaaagcgcc ctccttaatt tgtaaatctt aattgttttg
     6541 ttttttctct ttaggtaaga ttaagttcaa aataattcct gaaagtgcag ctaatgccat
     6601 cccctcaatt tgaaggttga taccaatttc ttttaagtta aatactaaat taccgatacc
     6661 tacaactaaa attacagaag ctataactaa atttcgattg ttcgcaaaat ctactttgct
     6721 ttcaactaac attcttaagc cacttgctgc aataataccg aaaagtaata tagatactcc
     6781 tcccataact ggtgtaggta tagaagaaat taatgcagtg aacttaccaa tgaatgctaa
     6841 aacaattgct ataactgctg caccaccaat gacgtaaata ctgtatattc tggtaatcgc
     6901 taatacaccg atattttcac cgtatgttgt acttggtggt ccaccaataa tactggcaaa
     6961 cattgtagaa acaccatcac caatgattga tttatcaagt cctggctttt caaagaagtt
     7021 tctacctacg attttattca ataccatttg gtgcccaata tgttcactta ctgttacgaa
     7081 cacaatcgga atcattacaa gtacaagtcc taagtgaaat gaaggtacat aatctttaaa
     7141 tggcagataa atatgaggga aatctatcca ttttgctgac ataattgcat caaatttcac
     7201 aatccccata aaaatcgcta ccacataacc tacgataata cctacaagaa ctggaattaa
     7261 agataggaat cctttaaaaa atccttgaac gactattgtt acaagcaagg ttatcattgc
     7321 aacaattaag aaactgatat tgtaaccttt catatctcca ggattttcat acattgccat
     7381 attgactgca gtaggcgcta agcttaaacc aattaccatg atgactggtc caacaacaac
     7441 tggtggtaat aatttcatta accatgctgt cccacttaat ttgattagaa tcccgatgat
     7501 gacgtacata acaccactca tgaataatgc tacaagcatg tctcctaagc tatgcgtact
     7561 taatcccgtg ataattggcg tgataaatgc aaagctagat cccaagtatg ctggtatttg
     7621 cgccttcgtt attaagatat aaagtaatgt accgattccc gaagctagta acgctgctga
     7681 tattggtagt cctgttaaga atggtactag tactgttgcg ccaaacatcg caaataaatg
     7741 ttgtaagctt aaaaatgccc attgcgctgg ttgtggtttt tcatttacat ctagtacggg
     7801 ttttactgtt cgttcaaaca tttcatcatt ttgcataata ttcatttcct ccgataataa
     7861 aaaaatctct ttacatcagt atatgtaaag agacaaaaag tgtgacaagt tgctacaagt
     7921 cattttcgtc catagaaatt gacttatagt tgtcgaacat gagggtatta ttagataaac
     7981 aagcatatga aaacttattt atcattcaac tcccccacct ttttcagtct ctcgtactga
     8041 attaaaaggg gtattattta attataactg catttctttg atccatttct tctaaataga
     8101 cacttaccgt ttcctcttta gaagtaggta tatttttacc aacaaaatct gctcgaattg
     8161 gtaactcacg atgtcctcga tcaaccaaag cagctaaacc aattttaata ggtctagcat
     8221 ttagcaaaat agcatcaagt gaagcacgaa ccgttcgacc agtatacagc acatcgtcaa
     8281 taatgatgac tactttatct gtaatatctg tgtcgatgtc tattgcgtct tttgtcgtaa
     8341 gtgatgacat gtgctctata tcatctctaa agtatgtaat atcaattgtt ccagtaggta
     8401 tacgttgttg ctcaatttga tgaattttat cttgtatacg attcgctaaa tattcacctc
     8461 ttgttttgat acctaaaaga attaaattat cagtaccttt gttatactcc aaaatttcgt
     8521 gagcgattct cgtcactgta cgttgtattg cggcatcatc cattatgata cgttcagaca
     8581 tattctcacc ctttcaaaat ttagaaacaa aaaacctcat gtctttaata tcgacatgag
     8641 gtttagtata tacaatcagc ctatttacat agaagaatcg tagtattact acttattctt
     8701 gcatatgtga aagtgttatg atcattttag tatatatcta tattttcatg tctttgacgt
     8761 ctctctggac gctcaattaa agactcttta tgttgtactt aaactgttat aaggataata
     8821 ctaatcaggc gctttgtcaa tctatattga aaaattaacc atactcagta aaaatgttaa
     8881 ccttaatcta ttttataatg tgttttcatt taaaaaaacg aaaacgataa acacttatag
     8941 tttacataat aatgttatcg taattatagc acgacacaat tatgcatctc tttttcgaat
     9001 tgtatctaat aaatcttcaa agtcttgtgg taattcagca tgtctttcaa tatattcact
     9061 tgttactgga tgttcgaatc caataagtcc agcatgtaga gcttgaccac caatatccaa
     9121 tgtctttttc ggtccatact ttggatcacc aactaatggg aagccaatat atttcatgtg
     9181 aacacggatt tgatgcgtac gtcctgtttc aagttgacat tcaacaagcg tataatcttt
     9241 aaaatgttct agtacgttaa aatgtgtcac tgcttcctta ccatcatcaa caacagccat
     9301 agattgacga tcatttttgt ttctaccaat tggcgcatcg attgtaccgt aatcatgagg
     9361 aatattcccg tgaactaaag cgatatattt tcttttaaca gatttatcca ttaattgttc
     9421 tacaagccca cgatgagcaa tatcattttt agcaaccatt aataaaccag aagtatccat
     9481 atctatacgg tgaacaatac ctggacgaat ttctccatta atacctgaca aatttttaat
     9541 ttgatacatt aaaccattaa ctaatgtatt ggtataatgc cctggtgatg gatgaactac
     9601 catgcctttc ggtttatata caactgcaac atcgtcatct tcataataaa tatctaaatt
     9661 taaattttca ggtagaatat cagcttcaac cacttctttt tcagtgacaa ctatatgatc
     9721 attaagtttc actttataat tagatttaac aactttatcg tttgcaacga ccaaacctgc
     9781 tttaatccaa tcttgtatct ggttacgaga ccaatcatta tttaattcag gcagcaactt
     9841 atctacacgc atacctgttt gttctttatc tgtaatgtta aattcataag tctccattac
     9901 ttaacctcct tctccttttt attggaagta tcctttaata aggcaataat aattaatatt
     9961 acaccaattg ttaaacttga atctgcgata ttaaatattg gaaaatcata accaaaaata
    10021 tttgtatcaa taaagtcaac aacttctcct gttaaaattc tatcaataaa gtttccaagt
    10081 gcacctgcaa aaagtaaact aatagcaact tgcataaaca aattatattg agcatcttta
    10141 ataaagaaat a
//
LOCUS       DAKTIO010000101         9972 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.101, whole genome shotgun sequence.
ACCESSION   DAKTIO010000101 DAKTIO010000000
VERSION     DAKTIO010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9972
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.101"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(228..3284)
                     /gene="fnbA"
                     /locus_tag="O0293_001811"
     CDS             complement(228..3284)
                     /gene="fnbA"
                     /locus_tag="O0293_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbA"
                     /protein_id="HCY0280694.1"
                     /translation="MKNNLRYGIRKHKLGAASVFLGTMIVVGMGQDKEAAASEQKTTT
                     VEENGNSATDNKTSETQTTATNVNHIEETQSYNATVTEQPSNATQVTTEEAPKAVQAP
                     QTAQPANIETVKEEVVKEEAKPQVKETTQSQDNSGDQRQVDLTPKKATQNQVAETQVE
                     VAQPRTASESKPRVTRSADVAEAKEASNAKVETGTDVTSKVTVEIGSIEGHNNTNKVE
                     PHAGQRAVLKYKLKFENGLHQGDYFDFTLSNNVNTHGVSTARKVPEIKNGSVVMATGE
                     VLEGGKIRYTFTNDIEDKVDVTAELEINLFIDPKTVQTNGNQTITSTLNEEQTSKELD
                     VKYKDGIGNYYANLNGSIETFNKANNRFSHVAFIKPNNGKTTSVTVTGTLMKGSNQNG
                     NQPKVRIFEYLGNNEDIAKSVYANTTDTSKFKEVTSNMSGNLNLQNNGSYSLNIENLD
                     KTYVVHYDGEYLNGTDEVDFRTQMVGHPEQLYKYYYDRGYTLTWDNGLVLYSNKANGN
                     GKNGPIIQNNKFEYKEDTIKETLTGQYDKNLVTTVEEEYDSSTLDIDYHTAIDGGGGY
                     VDGYIETIEETDSSAIDIDYHTAVDSEAGHVGGYTESSEESNPIDFEESTHENSKHHA
                     DVVEYEEDTNPGGGQVTTESNLVEFDEESTKGIVTGAVSDHTTVEDTKEYTTESNLIE
                     LVDELPEEHGQAQGPVEEITENNHHISHSGLGTENGHGNYDVIEEIEENSHVDIKSEL
                     GYEGGQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNKGNQSFEEDTEKD
                     KPKYEHGGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPSYQFGGHNSVDFEEDTLP
                     KVSGQNEGQQTIEEDTTPPIVPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEV
                     PSEPETPTPPTPEVPAEPGKPVPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVAPT
                     KKPQSKKSELPETGGEESTNKGMLFGGLFSILGLALLRRNKKNHKA"
     gene            complement(3268..3369)
                     /locus_tag="O0293_001812"
     CDS             complement(3268..3369)
                     /locus_tag="O0293_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280695.1"
                     /translation="MLLKTEQYRLAFIKFKKLMNFAFKGRYYSEKQS"
     gene            complement(3753..5111)
                     /locus_tag="O0293_001813"
     CDS             complement(3753..5111)
                     /locus_tag="O0293_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease"
                     /protein_id="HCY0280696.1"
                     /translation="MFNEIWPLISVVLGIVILLVLIIGFKLNTFISLIITSMITALML
                     GIPLTKIMETIEKGMGSTLGHIALIFGLGAILGKLLADGGGATRIADTLIQKFGQKHV
                     QWAMLVAAFIVGIALFFEVGLVLLIPLVFTVAKRANVSVLKLGLPMVTALSVTHGFLP
                     PHPGPVVIAKELKANVGDVLLYGMIIAIPVTLIAGPIFNKVAQKMIPSAYTREGDISA
                     LGAQKEFTDQEMPGFGMSLLTATLPVILMLVSTITQLVTGHDKPTNLFESIIYMIGTA
                     GTAMLIAVLFAIVTMGLMRKRKMNHIMESVTNAIYPIGMMLLIIGGGGTFKQVLIDGG
                     VGNTIAKMFEGTEMSPILLAWIVAAVLRIALGSATVAAISTTGIVLPLLQSSDVNVAL
                     VVLAIGAGSVILSHVNDAGFWMFKEYFGLTVKETFLTWSLLETIISVSGIIFILFISL
                     FV"
     gene            complement(5228..6781)
                     /gene="gntK"
                     /locus_tag="O0293_001814"
     CDS             complement(5228..6781)
                     /gene="gntK"
                     /locus_tag="O0293_001814"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCY0280697.1"
                     /translation="MKYMIGVDIGTTSTKSVLYDENGTFIMKHQIGYDLHTPNVDVSE
                     ENPDELFDAVLMTIKYVMRESKVNKDDIKFVSFSAQMHSLIAMDQQHQRLTNNITWAD
                     NRAAKYATVINEVHDGNAIYQRTGTPIHPMSPLAKIFWMKHEWQDVFQRTAKFADIKT
                     YIFYHLFDTYIIDYSMASATGMFNLETLDWDVEALELLGISKEMLPELVPTTYVMKGM
                     KERYATLMGLNKDTPFVIGASDGVLSNLGVNSVGKGEVAVTIGTSGAIRTVIDKPRTD
                     YKGRIFCYVLTEDHYVIGGPVNNGGVVLRWLRDELLASEVETAKRLGVDPYDVLTQIA
                     KRVKPGADGLIFHPYLAGERAPLWNANARGSFFGLTLSHKKEHMIRAALEGVLYNLYT
                     VYLALIEVMNETPKMIKATGGFAKSEVWRQMMSDIFDTELVVPESYESSCLGACVLGL
                     KAVGDIEDFSIVSSMVGATNNHTPIEENVTVYQEIVSIFINLSRSLTENYEQIADFQR
                     QHIAENKTQ"
     gene            complement(6805..7485)
                     /locus_tag="O0293_001815"
     CDS             complement(6805..7485)
                     /locus_tag="O0293_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCY0280698.1"
                     /translation="MMYGYPEKWLEGMTTGEGIAAELRLGIVNGHIAEGTLLTENQMA
                     KQFNVSRSPIRDAFKLLQQNQLIQLERMGAHVLPFGEQEKKEMYDLRLMLESFAFSRV
                     KNQERLPIVKEMKKQLEMMKVAVKFEDAESFTKHDFEFHETLIKASNHQYLNSFWSHL
                     KPVMMALVLTSMRQRMQQNPQDFERIHHNHQVFIDAVEQYDSQILKEAFHLNFDDVGK
                     DIEGFWLN"
     gene            complement(7639..8403)
                     /locus_tag="O0293_001816"
     CDS             complement(7639..8403)
                     /locus_tag="O0293_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCY0280699.1"
                     /translation="MSNYSTGELAKLCNVTTRTIQYYDRKGILKPQGFTEGKRRVYTE
                     QQRQTLELILLLKDLGCALSDIDMLLKGEGTLKTLNTLLTMKQQEINQQVKQQQAVLN
                     KIKNVQYYVNEASTSPITHLKDIEHVMSKSAEMKSIRRNIWISAGIIGIIQYSSIISA
                     ILMKNKWPFLIALPFMIGYGIGVTFYYQQKVAYLCPNCQHIFSPSLWAVIKAKHTATT
                     RRFECPNCHETHYCIEVPKAHMSTEQLEISHIQHNN"
     gene            complement(8568..9260)
                     /locus_tag="O0293_001817"
     CDS             complement(8568..9260)
                     /locus_tag="O0293_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCY0280700.1"
                     /translation="MYVDRKPSLYLEDLRHDFKNSLSKFENGDEAFDTLLGFVELDHI
                     YSSALKEISTKLSILDDNFNHIYKHNPIHHMERRVKEMRSLIEKLNRKGLQISAETAK
                     EHILDIAGIRVVCNYLDDIYLIEEMLLKQEDVQLIKRKDYIQHPKENGYRSLHIVVSI
                     PVFLAERVEVLPVEIQIRTIGMDMWASLEHKIRYKNNAETEKYRDLLKECATEITEVE
                     DKLQQIHSEITE"
     gene            complement(9491..9907)
                     /locus_tag="O0293_001818"
     CDS             complement(9491..9907)
                     /locus_tag="O0293_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCY0280701.1"
                     /translation="MPFTMKGYPEKWKEFEVIKRQKAIEIANAMLKEGYLEKDVVPIA
                     AKKAKDWYRALSKEEIKALEEENFVQFHTATMEKTEVDSEYATTDNEEHIQEQSNQDK
                     SYFDSESIGNQSGGVRQREPLMDGPHPKVVESFKDV"
BASE COUNT         2844 a         1998 c         1482 g         3648 t
ORIGIN      
        1 agttaataat tgcatttatc aaatttgtct tattgatcca atctaatttg tactcacaaa
       61 ctagtttaaa attctaactt tatctctcag ttcgttatca atcatcagac ataaaccaat
      121 gaagcaatca gaaaacactc taattttcta ttagaaattt gatttaatat aaaaaaacag
      181 gcttacttca tataatttat gaaataaacc cgtcaatttt tgtttaatta tgctttgtga
      241 ttctttttat ttctgcgtaa taatgctaaa cctagaatgc tgaataatcc gccgaacaac
      301 atacctttgt ttgttgattc ttctccacct gtttcaggta gttcagattt cttagattgt
      361 ggttttttag ttggtgccac tgctttaacc ttttcattga tttcaataac aggtgttact
      421 actttacctt gttccactgg tttagaaggc tttttaggtt cttctttggc aggtggtact
      481 ggtttaccag gttcagctgg tacctctggt gttggcggtg ttggagtttc tggctcactc
      541 ggcacttctg gtgtcggtgg tgttggtgtt tccggctcac ttggtacttc tggtgttggt
      601 ggcgttggtg tttccggctc acttggtact tctggtgtcg gtggcgttgg tggcacgatt
      661 ggaggtgttg tatcttcttc aatcgtttgt tgaccttcat tttggccgct tacttttgga
      721 agtgtatctt cttcaaagtc aacactattg tgtccaccga attgataact tggtttatct
      781 ttatttgtat cttcttcaat aatttcagtg tgcttattga atccgtgaat atgtggcaca
      841 ctgtcgaagt cgatatcaat gatgttaccg ccatgttcat acttaggttt gtctttttct
      901 gtatcttcct cgaatgactg attaccttta ttttgaccat gaatttgagg tacactatca
      961 aaatcgatat ctacgatatt gccaccttgt tcatatttag gtttgtcttc ttctgtgtct
     1021 tcctcgaatg actggttacc gctattttgg ccaccttcat aacctaattc actcttaata
     1081 tcaacgtggc tattttcttc gatttcttca atcacgtcat aattcccgtg accattttca
     1141 gttcctaaac cagaatgaga aatatgatga ttgttttcag taatttcctc gactggtcct
     1201 tgtgcttgac catgctcttc aggtaattca tccactaatt caatcagatt actttcagtt
     1261 gtatattctt tcgtatcttc aactgttgta tgatcgctca ctgcgccagt tacaatacct
     1321 tttgtagact cttcgtcaaa ttcaactaag ttagactcag tagtaacctg accaccacct
     1381 gggtttgtat cttcttcata ttcaacaaca tcagcgtgat gttttgaatt ttcatgtgta
     1441 gattcttcaa agtcaattgg atttgattcc tcagaggact cagtgtatcc tccaacgtga
     1501 cctgcttcgc tatccacagc agtatggtaa tcgatatcaa tagctgatga atccgtttct
     1561 tctattgttt caatgtatcc atcaacatat ccacctccac catctatagc tgtgtggtaa
     1621 tcaatgtcaa gagttgatga atcatattcc tcttcaacag tagttactaa attcttatca
     1681 tattgacctg taagagtttc tttaattgta tcttctttat attcaaattt attattttga
     1741 ataatcggac catttttccc atttccgttc gctttattac tgtataaaac taaaccatta
     1801 tcccaagtta aggtatatcc tctatcataa taatacttat aaagttgctc tggatgtcct
     1861 accatttgtg ttctaaaatc aacttcatca gtaccattta aatactctcc atcatagtga
     1921 acaacataag ttttatctag attttctata ttcaatgaat agcttccatt attttgtaaa
     1981 ttcaaattcc cactcatatt acttgtgact tctttaaatt tagaagtatc tgtcgtattt
     2041 gcatatacac tcttcgctat gtcttcatta ttacccaagt attcaaatat cctaactttt
     2101 ggttgatttc cattctgatt actacctttc attaaagttc cagtaacagt cacacttgtc
     2161 gttttaccat tattaggttt aataaatgca acatgcgaaa atctattatt cgctttatta
     2221 aatgtctcaa tcgatccatt taaattggca taataattcc caataccatc tttatattta
     2281 acatctaatt cctttgaagt ttgttcttca tttagtgttg aagttatagt ttgatttcca
     2341 ttagtttgta cagttttagg atcaataaat aaattaattt ctagttcagc cgttacatca
     2401 accttatctt caatatcatt tgtaaatgta tatctaatct ttccaccttc taaaacttca
     2461 cctgtcgcca ttacgactga accattttta atttctggta cttttctagc agttgatacg
     2521 ccatgcgtat ttacattatt tgataaagta aagtcaaagt agtcaccttg atgtaaacca
     2581 ttctcaaatt tcaacttata ttttagtacc gctcgttgtc ctgcatgagg ttctacttta
     2641 tttgtattgt tatgcccctc aatagaacca atttctactg taactttact tgttacatct
     2701 gtacccgttt ccactttcgc gttactagct tccttagctt ccgctacatc tgctgatctt
     2761 gtcacacgtg gcttactttc tgatgccgtt cttggctgtg ccacttcaac ttgtgtttct
     2821 gcgacttgat tttgtgtagc ctttttaggt gttaaatcta cttgtctttg atctccgcta
     2881 ttgtcttgag attgtgttgt ttccttaact tgaggtttcg cttcttcctt aactacctct
     2941 tctttaactg tttctatatt tgctggttgt gcagtttgtg gtgcttgtac tgcttttggt
     3001 gcttcttcag ttgttacttg tgttgcgttt gacggttgtt ctgttactgt tgcgttatat
     3061 gattgagttt cttctatatg attaacgtta gttgcagttg tttgtgtttc acttgtttta
     3121 ttatcagtag ctgaattccc attttcttct actgtagttg tcttttgttc tgatgctgca
     3181 gcttctttgt cttgtcccat cccaacaacg atcattgttc ctaagaatac tgatgctgct
     3241 cccaatttat gttttctaat gccgtaccta agattgtttt tcactataat atctcccttt
     3301 aaatgcaaaa ttcattaatt ttttaaactt aataaatgca agtctatatt gttcggtttt
     3361 taaaagcaat acaaaaaagc gagttaatga aaagttaaga ttgtcattaa ttttatgtta
     3421 attgataatt ttattatttg aaatatacct ataaattgta ttcaagtcat cagaaaccct
     3481 tgtcacacaa ggcttgtatt ttttatactt atttttttaa attaaattca tcattatcta
     3541 atttaaaaca atatactaaa cgtttcataa ttatcgcctg tacaatacgc acaaaaacat
     3601 gtcttgaaac gcctttcatc actctaaaat acccaatata ctttttatat cgttcggatt
     3661 ctgagtattt cagacgattt tctgcataaa aataaacgtg tttcaaggca atatattgca
     3721 attacctaaa aacacgttta cttaatattt agttaaacaa ataagctaat gaataaaatg
     3781 aagatgatac ctgaaacgga aataatcgtt tctaataatg accatgttaa gaatgtttct
     3841 tttacagtta aaccaaaata ttctttaaac atccaaaatc ctgcgtcatt tacatgagac
     3901 aaaatcacac tacctgcacc tatcgcaagt acaactaatg caacatttac atctgatgat
     3961 tgtaataatg gtaagacaat acctgtagtt gaaatcgcag ctactgtagc cgaacctaat
     4021 gcgatacgta gcacagctgc aacaatccat gctagtaaaa tcggagacat ctctgtacct
     4081 tcaaacattt tagcaattgt atttccgaca ccgccgtcaa ttaatacttg tttaaatgta
     4141 ccgccaccgc caataatcaa taacatcatt ccgattggat aaatcgcatt cgtcactgat
     4201 tccataatat gattcatctt acgctttctc attaatccca tcgtaacgat tgcaaataat
     4261 actgctatta gcatggctgt ccctgctgtt cctatcatat aaatgataga ttcaaataga
     4321 tttgtaggtt tgtcatgccc agttacaagt tgcgttatcg tagacactaa cattaatatg
     4381 actggtaatg ttgctgttaa taaactcata ccaaatcctg gcatctcttg atccgtaaat
     4441 tctttttgtg cacctaacgc tgaaatatcg ccttctcgtg tatacgcaga cggaatcatt
     4501 ttttgtgcaa ctttgttaaa tataggccct gcaatgagtg taactggaat ggcaataatc
     4561 ataccataca gtaatacatc tccaacattt gcctttaatt cttttgcgat gactaccggt
     4621 cctggatgtg gtggtaaaaa gccatgtgtc actgataaag ctgttaccat aggtagtcct
     4681 agttttaaca ctgaaacatt tgcgcgtttt gctactgtaa atactaatgg aatcagtaag
     4741 actaaaccta cttcaaagaa caatgcaata ccgacgataa atgctgcaac aagcattgcc
     4801 cattgtacat gtttttgacc aaatttttga atcaacgtgt ctgcgattcg agttgcacca
     4861 ccaccatcag caagcaattt cccaagtatg gcacctaaac cgaatatcag tgcaatgtgg
     4921 ccgagcgtac tgcccattcc tttctcaatc gtctccataa ttttagtcaa tggtatacct
     4981 agcattaacg ctgtaatcat cgatgtgata attaatgaaa taaatgtatt taatttaaac
     5041 ccaataatta atactaataa aataacgata cctaaaacaa cactgattaa cggccatatt
     5101 tcgttaaaca tgacattccc ctctttctct tttcaataga atgtaacacc gtcgtcgcgc
     5161 acgtattcca cataatatat agtagtcatg gcacaacaac tttatctaag atgcttgagt
     5221 gacgtattta ttgtgtttta ttttcagcga tatgttggcg ttgaaaatct gcaatttgtt
     5281 cataattctc tgttaaagaa cgacttaaat tgataaaaat ggatacgatc tcttggtaaa
     5341 cagtgacatt ttcttcaatc ggcgtatgat tgtttgtggc accgaccatc gatgaaacga
     5401 ttgaaaaatc ttcaatgtca cctacagctt taagtccgag cacgcaggca cctaagcatg
     5461 aactttcata actttcagga accactaact ctgtgtcaaa tatatctgac atcatttgac
     5521 gccatacttc acttttcgca aaaccacctg ttgcttttat catcttaggt gtttcattca
     5581 ttacttcaat aagcgcaaga tagacggtat acaaattgta aagaacacct tctaatgcag
     5641 cgcgaatcat atgttctttt ttatgagata aagttaaacc gaagaatgaa cctcttgcat
     5701 ttgcgttcca aagcggcgca cgttctcctg ctaaataggg atggaatatt aaaccatctg
     5761 cacctggttt aacacgcttt gcaatttgag ttaagacatc ataaggatca acaccgagac
     5821 gtttcgcagt ttcgacttca ctcgctagca actcgtcgcg caaccatctc aatacgacac
     5881 caccattatt tacaggacct ccgatgacgt agtggtcctc tgttaagaca taacaaaata
     5941 ttctaccttt gtaatcagta cgcggtttat ctatcacagt acgaatcgcc ccagatgtac
     6001 cgattgtgac agcaacttct cctttaccaa cactattgac acctaaatta gaaaggaccc
     6061 catcactcgc accaataaca aacggtgtat ctttattaag ccccattaat gttgcataac
     6121 gttctttcat acctttcatc acatacgttg ttggaactaa ttccggcaac atttccttgg
     6181 aaatacccag cagttctaat gcctcaacat cccaatctaa tgtttctaaa ttaaacatcc
     6241 ctgttgcgga agccattgaa taatcaatga tatatgtatc aaataaatga tagaaaatgt
     6301 atgttttaat atctgcaaac ttagcagtac gttgaaatac atcttgccat tcatgtttca
     6361 tccaaaaaat cttcgctaat ggcgacatag gatgaatcgg tgtgcctgtt cgctggtaaa
     6421 tcgcattgcc atcatgcact tcatttatta ctgttgcata ttttgcagcg cggttatctg
     6481 cccaagtaat attatttgtt aatctttgat gttgctgatc catcgcaatc aagctatgca
     6541 tttgcgcact aaatgacaca aacttaatgt cgtctttatt aactttggat tctctcataa
     6601 catatttaat agtcattagt actgcatcaa ataattcatc tgggttttct tctgagacat
     6661 caacgtttgg tgtgtgtaaa tcatagccta tttgatgttt catgataaaa gttccatttt
     6721 catcatataa gactgacttg gtactcgtcg ttccaatgtc gacaccaatc atatatttca
     6781 tgataaatcc ttctttcttt cattttaatt caaccaaaat ccttcaatat ctttaccaac
     6841 atcgtcgaaa tttaaatgaa acgcttcttt caaaatttga ctgtcgtatt gttccactgc
     6901 atcaataaac acttgatgat tatgatgtat gcgttcaaaa tcttgcgggt tctgttgcat
     6961 acgttgtcgc atggatgtta aaacgagtgc catcattact ggttttaaat gactccaaaa
     7021 tgagtttaaa tattgatgat tagatgcttt gattaatgtt tcatgaaatt caaagtcatg
     7081 cttcgtaaat gattctgcat cctcaaattt tactgccact ttcatcattt caagttgttt
     7141 cttcatttct tttacgatgg gtagtcgctc ttgattttta actcttgaaa atgcaaatga
     7201 ctctaacatc agtcgcaaat catacatttc tttcttttct tgttccccaa acggcaacac
     7261 atgtgcaccc attctttcta attggatgag ttgattttgt tgcaataatt taaatgcatc
     7321 tcgaattggc gaacgactca cattaaattg ctttgccatt tgattttcag tgagtaacgt
     7381 accttcagct atgtgaccat tcacaatgcc taagcgtaat tctgccgcga taccttctcc
     7441 agttgtcata ccttccaacc atttctctgg atatccatac atcatcaaag tcactccttc
     7501 attacacgac atacttgtat acaagtatgt taatatagtt attatgagtt tgcaagcgct
     7561 ttctttacga gcactaaaat agtgaccacc ccttttcgat ttaaatttaa aggaaatggt
     7621 cactatcaca cgaatgattt aattgttatg ttgtatgtgg gatatttcta attgttctgt
     7681 actcatatgc gctttaggta cttcaatgca ataatgcgtt tcatgacagt ttggacattc
     7741 gaatcgacgt gttgtcgctg tatgtttcgc tttgataact gcccacaaag atggtgagaa
     7801 tatatgctgg cagttaggac ataaataggc aaccttttgt tggtaataaa aagtaacacc
     7861 aatgccataa ccaatcataa atggtaaagc aattaaaaac ggccatttat ttttcatcaa
     7921 aattgcactt ataatgctag aatattgaat tattcctata ataccagcac taatccaaat
     7981 gttacgacga atacttttca tttcagctga tttactcatg acatgctcta tgtcttttaa
     8041 gtgtgtgatt ggagacgtcg acgcttcatt tacgtaatat tgaacatttt taattttgtt
     8101 taataccgct tgttgctgtt taacttgttg gttaatttct tgttgtttca tagttagtaa
     8161 agtattgagc gtcttcaaag taccttcacc ttttagcaac atatctatat cgcttaacgc
     8221 acaacctaaa tctttaagca ataagattaa ctctaatgtt tgtcgctgtt gttctgtata
     8281 cacacgacgc tttccttctg taaatccttg tggtttcaaa atacctttgc gatcataata
     8341 ttgaatcgtt cgtgttgtca cattgcataa ttttgcgagt tctccagtcg aatagttaga
     8401 catagattcc acctcctata attaccatag ttgatgaccc gacgtcacga gcaagtacaa
     8461 tttccacatt ttaaagaaat ttattatact aggcgtctta tttttatgat ttcgtaccat
     8521 gttgatttac aaactcactc aaactaagta acacacctac taaacatcta ctctgttatt
     8581 tcagaatgaa tttgttgtaa tttatcttca acttcagtaa tctctgtcgc acattctttc
     8641 agtaaatctc gatacttttc cgtctctgca ttgtttttat aacgtatttt atgttctaaa
     8701 cttgcccaca tatccatacc tatcgttcta atttgaattt caacaggcaa tacctcaacg
     8761 cgttctgcta aaaagactgg aatagataca acgatatgta aactgcggta accattttct
     8821 ttagggtgct gaatataatc tttacgtttt atcaattgta cgtcttcttg tttaagcaac
     8881 atctcttcaa tcaaataaat atcatctaag taattacata ctacgcgaat tccggcaata
     8941 tccagtatat gttctttggc agtttctgcg ctaatctgta atcctttacg attaagcttt
     9001 tctattaaac tacgcatttc tttcacacgt cgctccatat gatgtatagg attgtgttta
     9061 taaatgtgat tgaaattgtc atctaaaata ctcagtttag tgcttatttc ctttagtgct
     9121 gacgaataaa tatgatctaa ctctacgaaa cctaataacg tatcaaatgc ttcatcacca
     9181 ttttcaaatt tacttaagct atttttaaaa tcatgtcgca aatcctctaa atatagtgat
     9241 ggttttcgat ctacatacat ttttatacta acctccgata tataatcatc tttattgtac
     9301 ctaacatttt attaagataa tactaataca ctatcgaact ttgggctgat actgaaacag
     9361 cacaaagaaa ctcaaacaat tcgaattatg tatcaaaacc ttcaattaca ggaagtgatt
     9421 ttaaaatgat ttaacgcaaa aaaacacctg ttaccgttat ataggtacaa gtgcttaatt
     9481 gatagagatg ttatacgtct ttaaatgatt ccacaacttt tggatgtgga ccatccataa
     9541 gcggttctct ttgtcgaacg ccaccacttt ggttgccaat tgattcgcta tcaaagtacg
     9601 atttatcttg atttgattgt tcttgaatat gttcttcatt atcagttgtt gcatattcac
     9661 tatccacctc tgttttttcc attgttgctg tatggaattg tacgaagttc tcttcttcta
     9721 atgctttgat ttcttctttc gataacgctc gataccaatc tttcgctttt ttcgccgcaa
     9781 taggaacaac atctttttca agatatcctt cttttaacat tgcattcgca atttctatcg
     9841 ctttttgccg tttaattact tcaaattctt tccatttttc ggggtaacct ttcattgtaa
     9901 agggcatttc cttaacctcc aatatgttat aattcatgtt atataccacc ataattataa
     9961 aatgaaacat at
//
LOCUS       DAKTIO010000102         9617 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.102, whole genome shotgun sequence.
ACCESSION   DAKTIO010000102 DAKTIO010000000
VERSION     DAKTIO010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9617
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.102"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            341..1093
                     /locus_tag="O0293_001819"
     CDS             341..1093
                     /locus_tag="O0293_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HCY0280702.1"
                     /translation="MGEFEVKYLTGFILILLLEGIFTNSASAIEYSDLHHKSKFDSKR
                     LSNAKMSFINPTQLENKNTNDRLLKHDLLFHDMFVNDDWKKDFKVEFENEALSKKFIN
                     KDIDIFAGNYGYGCHGGATNKTQCSYGGVTLSDNNKYDDYKNIPCNLWIDGHQTEIEL
                     TAVKTKKKIVTIQELEVQLRNYLNEKYKLYEQGGDIVKGYVKYYNDDEQNVEYDFYNL
                     NGEYGREVLKMYADNKTINSDKLHLDIYLFKS"
     gene            complement(1295..1564)
                     /locus_tag="O0293_001820"
     CDS             complement(1295..1564)
                     /locus_tag="O0293_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280703.1"
                     /translation="MAENNQNSLVTKIATYGSFIAIASFVILFISIFLKFSFNIEFTA
                     TIMNICRYTLILGFILMSLPDVVDKNIKKIIFDAFIIIVFIFFLL"
     gene            1878..2564
                     /gene="mtnN"
                     /locus_tag="O0293_001821"
     CDS             1878..2564
                     /gene="mtnN"
                     /locus_tag="O0293_001821"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCY0280704.1"
                     /translation="MIGIIGAMEEEVTILKNKLTQLSEISVAHVKFYTGILKDREVVI
                     TQSGIGKVNAAISTTLLINKFKPDVIINTGSAGALDESLNVGDVLISDDVKYHDADAT
                     AFGYEYGQIPQMPVAFQSSKPLIEKVSQVVQQQQLTAKVGLIVSGDSFIGSVEQRQKI
                     KKAFPNAMAVEMEATAIAQTCYQFNVPFVVVRAVSDLANGEAEMSFEAFLEKAAVSSS
                     QTVEALVSQL"
     gene            2584..3111
                     /locus_tag="O0293_001822"
     CDS             2584..3111
                     /locus_tag="O0293_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HCY0280705.1"
                     /translation="MGLVRKFFMPNSYVQSIFQIDLDKLVDKGVKGIITDLDNTLVGW
                     DVKEPTERVKAWFKEANEKGITITIVSNNNESRVASFSQHLDIDFIFKARKPMGKAFD
                     KAITKMNIRPDQTVVIGDQMLTDVFGGNRRGLYTIMVVPVKRTDGFITKFNRLIERRL
                     LRHFSKKGYITWEEN"
     gene            3112..4212
                     /gene="yqeH"
                     /locus_tag="O0293_001823"
     CDS             3112..4212
                     /gene="yqeH"
                     /locus_tag="O0293_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HCY0280706.1"
                     /translation="MSDILKCIGCGAPLQSEDKNKPGFVPEHNMFRDDVICRRCFRLK
                     NYNEVQDVGLESEDFLKLLSGLADKKGIVVNVVDVFDFEGSFINAVKRIVGNKKIILA
                     ANKLDLLPKQINKRRVKEWLKRTARKYGLEADDVVLISAEKGWGIDDLLSSIANIREN
                     EDVYIVGTTNVGKSTLINKLIEASVGEKDVVTTSRFPGTTLDMIDIPLDETSFMYDTP
                     GIIQDHQMTHLVSEKELKIIMPKKEIKQRVYQLNEAQTLFFGGLARIDYVSGGKRPLV
                     CFFSNDLNIHRTKTEKANDLWRNQLGDLLTPPGNPQNFDLNEVKAVRLETGKEKRDVM
                     ISGLGFITIGPGAKVIVRVPKNVDVVLRNSIL"
     gene            4226..5032
                     /gene="aroE"
                     /locus_tag="O0293_001824"
     CDS             4226..5032
                     /gene="aroE"
                     /locus_tag="O0293_001824"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCY0280707.1"
                     /translation="MKFAVIGNPISHSLSPVMHRANFNSLGLDDTYEALNIPIEDFHL
                     IKEIISKKELEGFNITIPHKERIIPYLDYVDEQAINAGAVNTVLIKDGKWIGYNTDGI
                     GYVKGLHSVYPDLENAYILILGAGGASKGIAYELAKFVKPKLTVANRTMARFESWNLN
                     INQISLADAEKYLAEFDIVINTTPAGMAGNNESIINLKHLSPNTLMSDIVYIPYKTPI
                     LEEAERKGNHIYNGLDMFVYQGAESFKIWTNKDADINSMKTAVLQQLKGE"
     gene            5036..5326
                     /gene="yhbY"
                     /locus_tag="O0293_001825"
     CDS             5036..5326
                     /gene="yhbY"
                     /locus_tag="O0293_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCY0280708.1"
                     /translation="MLTGKQKRYLRSLAHNIDPIFQIGKGGINENMIKQIDDTLENRE
                     LIKVHVLQNNFDDKKELAETLSEATHSELVQVIGSMIVIYRESKDNKEIELP"
     gene            5329..5898
                     /gene="nadD"
                     /locus_tag="O0293_001826"
     CDS             5329..5898
                     /gene="nadD"
                     /locus_tag="O0293_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HCY0280709.1"
                     /translation="MKKIVLYGGQFNPIHTAHMIVASEVFHELQPDEFYFLPSFMSPL
                     KKHHDFIDVQHRLTMIQMIIDELGFGDICDDEIKRGGQSYTYDTIKAFKEQHKDSELY
                     FVIGTDQYNQLEKWYQIEYLKEMVTFVVVNRDKNSQNVENAMIAIQIPRVDISSTMIR
                     QRVSEGKSIQVLVPKSVENYIKGEGLYEH"
     gene            5888..6472
                     /gene="yqeK"
                     /locus_tag="O0293_001827"
     CDS             5888..6472
                     /gene="yqeK"
                     /locus_tag="O0293_001827"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HCY0280710.1"
                     /translation="MNIEKAKRLAKEKLPEKRYNHSLRVAETAIKLAEIYDGDTSKVE
                     LAGVLHDFCKYDDLGKMYQIVRQYELGNDLLSYGSEILHGPVCAAIMEHEYGINDEEV
                     LMAIKYHTTGRQQMTKTEKLIFIADYIEPGRTIPGVDDIRDMAYNQGSLDKTIYEISK
                     RTVLFLIQKDITVYNKTIDCLNYYNYSDERIKDD"
     gene            6473..6826
                     /gene="rsfS"
                     /locus_tag="O0293_001828"
     CDS             6473..6826
                     /gene="rsfS"
                     /locus_tag="O0293_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCY0280711.1"
                     /translation="MNSQELLAIAVDAIDNKKGEDTISLEMKGISDMTDYFVVTHGNN
                     ERQVQAIARAVKEVANEQNIEVKRMEGYNEARWILIDLADVVVHVFHKDERNYYNIEK
                     LYQDAPLESYSQVAY"
     gene            6829..7545
                     /locus_tag="O0293_001829"
     CDS             6829..7545
                     /locus_tag="O0293_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0280712.1"
                     /translation="MSQYAEMSLVYDQLTQDQPYEKWFEIVKNHCKDESNILDIGCGT
                     GSLTVQLEALGNVTGMDLSVDMLTVAAQKSANVNWLEGDMTSFDLQQQFDIITIFCDS
                     LNYLQDETAVIETFINVYHHLTDSGVFIFDVHTVYKMMTLFNNQSYIDDKGDIFLAWD
                     AVQGDLPLSVYHDMTFFIRHEDETYSRFDESHFQRTFDEKTYLSWLAQVGFKHVETFT
                     DFNIDEHNEDAERLFFIAKK"
     gene            7585..8271
                     /locus_tag="O0293_001830"
     CDS             7585..8271
                     /locus_tag="O0293_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HCY0280713.1"
                     /translation="MVLLYQFLLRYKDFLTQWKLYILSAVVLIMVLIGFIFWRQDDYT
                     SRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSKNKGPVYVDVKGAVKHPNVYKMT
                     SKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHEKN
                     GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKT
                     FDKLKSYFTT"
     gene            8363..8824
                     /locus_tag="O0293_001831"
     CDS             8363..8824
                     /locus_tag="O0293_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HCY0280714.1"
                     /translation="MERIKWEEYFMAQSHLLALRSTCQRLSVGATIVKDNRIIAGGYN
                     GSVAGEVHCIDEGCLIEDGHCIRTIHAEMNALLQCAKQGVSTEGATIYVTHFPCLNCT
                     KSIIQAGIKRIYYAEDYHNHEYATKLLKQSGIEFKKIPFSPEYVAKYLTKG"
     gene            8829..>9617
                     /locus_tag="O0293_001832"
     CDS             8829..>9617
                     /locus_tag="O0293_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family DNA internalization-related
                     competence protein"
                     /protein_id="HCY0280715.1"
                     /translation="MLFVALSMIVGVLWNSSKVLSTFLFILLLYITYRKNKIVYAPIS
                     LFLIIFSAWYLHYSQQAIFNYINYIERNSQFNERAQVIQIQRQGSDTYKGRLSLKNEI
                     YPFFLTDKKNFDLKKIESRNCIVKGQFKVNDNKFVTLKLQSIVVQSCLESNRSNLIEK
                     HKQFIMNRIYDSGIKFPDRIMALITGDVKEVNEQFKERVKEIVIYHLLAVSGSHIAAI
                     VFLIYQPLKRLNLPLFVIKGITIIVLALFAQYTNYAPSAVRAIIM"
BASE COUNT         3549 a         1177 c         1788 g         3103 t
ORIGIN      
        1 ttgagagtgg tgcatgacaa atgtactgct ctttttgttg tgtatcagta ttgtgatgtt
       61 ttaatgagaa tattatatga atcattatga aatttaataa aaataaaaga aatgattatc
      121 attttttctt atatactgtt aaacggtttg gaatttttag gtatacactg tattggttga
      181 tataactcaa ctaataattg cgaacagagt atttcaaatt gaaaagtatt atgagcgtga
      241 tacataatca aaattgtagg ctcaagaacc actacataat aaaccataag cggttcttta
      301 tcatttatgt ctcgctctca aatgtaaatt aataattgtt ttgggggagt ttgaagttaa
      361 atatttaaca ggatttattt taatattatt gttagaagga atttttacaa attcagcgag
      421 tgcaatcgaa tattcagact tacatcataa aagtaagttt gattcaaagc gtctaagtaa
      481 tgctaagatg tcattcatca atccaactca gcttgaaaat aaaaacacaa acgatagact
      541 gttgaagcat gatttgttat ttcatgacat gttcgtaaat gatgattgga aaaaggattt
      601 taaagttgaa tttgaaaatg aggcactttc aaagaaattt ataaataagg atatcgatat
      661 atttgctgga aattatggat acggatgtca tgggggagca accaataaaa cgcaatgtag
      721 ttatggtggt gttactttaa gtgacaataa taaatacgat gattataaga atataccttg
      781 taatttatgg attgacggac atcaaacaga aatagaacta actgcagtaa aaacgaaaaa
      841 gaaaattgtt actattcaag aattagaggt tcaattaaga aattatttga atgagaagta
      901 taagttgtac gaacaaggtg gcgacattgt taaagggtat gttaaatatt ataatgatga
      961 tgaacaaaat gtagaatatg atttttataa tttaaatggt gagtatggtc gtgaggtatt
     1021 aaaaatgtat gctgataata aaactatcaa tagtgacaaa ttgcatttag atatctattt
     1081 attcaaatca taatttaatt aagttaatca ttaataatct ttgacagtga aatataaagt
     1141 acctgtcatt atgttgttac aaataaaaac agcagtaagg atgctaacac ttagaaaagt
     1201 cttactgctg ttttatatta gaattatctt taatgcacaa aggaattcga tacgaaacaa
     1261 atatgattaa ctgtgtaatt acaaaataaa actactatag taaaaagaag ataaagacga
     1321 tgataataaa tgcatcgaat ataattttct tgatgttttt atcaacaaca tcaggtaagg
     1381 acataagtat aaatcctaga attagagtgt agcgacatat gttcataatt gttgctgtaa
     1441 actcgatatt aaatgaaaac tttaaaaata tacttataaa taaaatgaca aacgacgcaa
     1501 ttgcaataaa actgccgtat gtagctatct ttgttacgag actattttga ttattttcag
     1561 ccattgtaaa atcctcttat ttcatcatta ttattttaaa catatcattt tcctgttatt
     1621 taataaagtt tcttgacacg attggttgat gtaattgata tgattagttt ttgaaaaata
     1681 gacaaattag aagcatcgaa ttcactgtta tgagttcaaa aataattatt tatgatacca
     1741 agttattgaa agagcttttt ataaaaataa agcaaaagtt ttatacagta atgaaaatca
     1801 tgatacttgt cgttgtagtg attaaaatgt gataaaataa caaatgctag gattttttaa
     1861 aagaggtgaa tcaaaagatg attggtataa ttggtgccat ggaagaagaa gtaacaatat
     1921 taaaaaataa attaacacaa ttaagcgaaa tttcagttgc acatgttaaa ttttatactg
     1981 gcattttaaa agatagagaa gtagtgatta cccaaagtgg cattggaaaa gttaatgctg
     2041 caatttctac gacattatta attaataagt ttaaaccgga cgtcattatt aatacaggtt
     2101 ctgctggagc tttagatgaa agtttaaatg taggtgacgt tcttataagt gatgatgtaa
     2161 aatatcatga tgcagacgca acagcatttg gttatgaata tggacaaata ccacagatgc
     2221 cggtagcatt tcaatcaagt aaacctttaa tagaaaaagt atctcaagtt gtacaacaac
     2281 aacaattaac agctaaagta ggcttaattg taagtggtga tagctttatc ggtagtgttg
     2341 aacaacgcca aaaaattaaa aaagcatttc caaatgcgat ggcggttgaa atggaagcaa
     2401 ctgcaattgc acaaacatgt tatcaattta atgtaccatt tgttgtagtt cgtgcagttt
     2461 cagacttagc aaatggagaa gcggaaatga gcttcgaagc atttttagaa aaagcagctg
     2521 tatcatcaag tcaaactgtt gaagcattag tgtctcaatt ataaaaaggt aaaggtgagt
     2581 taaatgggtt tagttcgcaa gttttttatg ccgaattcat atgttcaatc aatatttcaa
     2641 attgatttag acaagttagt ggacaaaggc gttaaaggta ttattacaga tttagataat
     2701 acgctagtag gttgggatgt taaagaacct acagaacgtg ttaaagcatg gtttaaggaa
     2761 gctaatgaaa aaggaatcac tattacaatc gtgtctaata ataatgagtc tcgtgttgct
     2821 agttttagtc agcatttaga catcgatttt atttttaaag cgagaaagcc aatggggaaa
     2881 gcgtttgata aagcaataac taagatgaat atcagaccag atcaaactgt tgttataggt
     2941 gaccaaatgc ttactgatgt atttggtggt aatcgtcgag gtctatatac aattatggtt
     3001 gttccagtta aacgaactga tggctttatt actaagttta atagattaat tgaaaggcga
     3061 ttattacgtc atttcagtaa aaaaggttat atcacatggg aggaaaattg attgtctgac
     3121 attttaaaat gtatcggttg tggtgcgcca cttcaatctg aagataaaaa taaacctggt
     3181 tttgtaccag agcataatat gtttcgtgat gacgtgattt gcagacgttg tttccgcttg
     3241 aaaaattata acgaagttca agatgtagga ttagaaagtg aagacttttt aaaattatta
     3301 tcaggacttg cggataaaaa gggtattgtc gtcaatgtcg tggatgtatt tgactttgaa
     3361 ggatcattta ttaatgcagt taaacgtatt gtcggaaaca aaaaaatcat tttagcagca
     3421 aataaattgg atttattgcc gaaacaaatt aataaacgtc gagttaaaga atggttaaaa
     3481 cgaacagcaa gaaaatatgg tttggaagct gacgatgtcg tattaatttc agctgaaaaa
     3541 ggctggggca tagacgactt attatcatca attgcgaata ttcgagaaaa tgaagatgtg
     3601 tatattgtag ggacaacgaa tgttgggaaa tctacattga ttaataaact gattgaagct
     3661 agtgttggtg aaaaagatgt agtaacaact tcaagattcc ctggaacaac tttagatatg
     3721 atagatattc ctttagatga aacatcattt atgtatgata caccaggtat tattcaagat
     3781 caccaaatga cgcatttagt tagtgaaaaa gaattgaaaa ttattatgcc taagaaagaa
     3841 ataaaacaac gcgtatatca attaaatgag gcgcagacat tattcttcgg cggtctagcg
     3901 cgcatagatt atgtatcagg tggtaaacgt ccgttagttt gtttcttttc taatgacttg
     3961 aatatacatc gtactaaaac ggagaaggct aatgatttat ggcgtaatca acttggcgat
     4021 ttattaacgc cacctggaaa tccacaaaat tttgatctta atgaggtaaa ggctgttaga
     4081 cttgaaacag gcaaagagaa acgcgatgtt atgatctctg gtctaggctt tataactata
     4141 ggaccagggg ctaaagtaat cgttcgtgtt cctaaaaatg ttgatgttgt attaagaaat
     4201 tctattttat aaggtgatta aaaaaatgaa atttgcagtt ataggaaatc ctatttcaca
     4261 ttccttgtcg cccgttatgc atagagcaaa ttttaattct ttaggattag atgatactta
     4321 tgaagcttta aatattccaa ttgaagattt tcatttaatt aaagaaatta tttcgaaaaa
     4381 agaattagaa ggctttaata tcacaattcc tcataaagaa cgtatcatac cgtatttaga
     4441 ttatgttgat gaacaagcga ttaatgcagg tgcagttaac actgttttga taaaagatgg
     4501 caagtggata gggtataata cagatggtat tggttatgtt aaaggattgc acagcgttta
     4561 tccagattta gaaaatgcat acattttaat tttgggcgca ggtggtgcaa gtaaaggtat
     4621 tgcttatgaa ttagcaaaat ttgtaaagcc caaattaact gttgcgaata gaacgatggc
     4681 tcgttttgaa tcttggaatt taaatataaa ccaaatttca ttagcagatg ctgaaaagta
     4741 tttagctgaa ttcgatatcg ttattaatac aacaccagcg ggtatggctg gaaataacga
     4801 aagtattatt aatttaaagc atctttctcc caatacttta atgagtgata ttgtttatat
     4861 accgtataaa acacctattt tagaggaagc agagcgcaag ggaaaccata tttataatgg
     4921 cttagatatg tttgtttacc agggtgcgga aagctttaaa atttggacta ataaagatgc
     4981 tgatattaat tctatgaaaa cagcagtttt acaacaatta aaaggagaat aacatatgct
     5041 tactggcaaa caaaaaagat acttaagaag tttagcacac aatattgatc cgatttttca
     5101 aattggaaaa ggcggtatca acgaaaatat gattaaacaa atagatgata cgttagaaaa
     5161 cagagaattg attaaagtac atgtactaca aaataacttt gatgataaaa aagaattagc
     5221 tgaaacatta agcgaagcta ctcatagtga attagtgcaa gtgattggat ctatgatagt
     5281 gatttataga gaatctaaag ataataaaga aattgaattg ccataataat gaaaaagata
     5341 gtactttacg gcggtcagtt taaccctatc catactgcac atatgatagt agctagcgaa
     5401 gtatttcatg aattacagcc agatgaattt tattttttac ctagttttat gtctccattg
     5461 aaaaagcacc atgattttat agacgttcag cacagattaa caatgataca gatgattatc
     5521 gacgagcttg gttttggaga tatttgtgac gatgaaatta aacgtggtgg tcaaagttat
     5581 acctatgaca cgatcaaggc attcaaggag caacacaaag acagtgagtt gtactttgtt
     5641 attgggacgg atcagtataa ccaactagag aaatggtatc aaattgaata cttaaaagaa
     5701 atggttactt ttgtagttgt aaatcgagac aaaaatagtc aaaatgttga aaatgctatg
     5761 attgcaattc agatacctag ggtagatata agttcgacaa tgattcgaca aagagttagt
     5821 gaagggaaat ctatccaagt tcttgttcct aaatccgttg aaaactatat taagggggaa
     5881 ggattatatg aacattgaaa aagcaaaacg gcttgcaaaa gagaaactgc cagagaaacg
     5941 ttataatcat tctttaagag ttgcagaaac agcgattaaa ttggcagaaa tttatgatgg
     6001 agacactagt aaagtagaat tagcaggtgt attacatgat ttctgtaaat atgatgattt
     6061 aggtaaaatg tatcaaattg ttcgacaata cgaattaggt aatgatctac tgagttatgg
     6121 tagtgaaata ttgcatggcc ctgtgtgtgc agcaatcatg gaacatgaat atggtatcaa
     6181 tgatgaggaa gtattaatgg ctatcaaata ccatactact ggacgtcaac aaatgacaaa
     6241 aactgaaaaa ctgattttta ttgcagatta catcgaacct ggaagaacaa tcccaggagt
     6301 tgatgatatt cgagatatgg catacaatca aggtagttta gataagacaa tttatgaaat
     6361 ttctaaacgc acagtactat ttttaataca gaaagatatt acggtatata ataagacgat
     6421 tgactgttta aattattata actatagtga cgaaagaata aaggatgatt aaatgaattc
     6481 acaagaatta ttagcaattg ctgtggatgc aattgacaat aaaaaaggcg aagatacgat
     6541 ttctttagaa atgaaaggta tcagcgatat gacagattat tttgttgtaa cgcacggaaa
     6601 taatgaacga caagttcaag cgattgctag agcggtgaaa gaagtagcca atgaacaaaa
     6661 tatagaagta aaacgtatgg aaggatacaa tgaagcgcgt tggatattaa ttgacttagc
     6721 tgatgttgtg gtacatgttt tccataaaga cgaaagaaat tattataata ttgaaaagtt
     6781 atatcaagat gcaccattag aatcatatag tcaggttgcg tattaattat gtcacaatat
     6841 gcagaaatga gcctagtgta cgatcaattg actcaagatc aaccatatga aaaatggttt
     6901 gaaattgtaa aaaatcactg caaagatgaa tcaaatattt tagatattgg atgcggtact
     6961 ggtagtttaa cagttcaatt agaagcttta ggtaatgtta ctggtatgga tttaagtgtt
     7021 gatatgttga ctgttgcagc tcaaaaatca gcaaatgtaa attggcttga aggagatatg
     7081 acatcttttg atttgcaaca acaatttgat atcatcacta ttttttgtga ctctctaaat
     7141 tatttgcaag atgaaactgc agtgattgaa acattcatca atgtttatca tcatctgact
     7201 gattcaggtg tatttatttt tgatgtacat actgtttata aaatgatgac tttgtttaat
     7261 aatcaaagtt atattgatga taaaggggac atttttttag catgggatgc tgtgcaagga
     7321 gatttacctt taagtgttta tcatgatatg acatttttca ttcgacatga agatgaaaca
     7381 tattcaagat ttgatgagtc tcattttcag agaacatttg atgaaaagac atatttgtct
     7441 tggttagctc aagttggttt taaacatgtt gaaacattta ctgattttaa tatagatgaa
     7501 cataatgaag atgcagaaag attgtttttc attgcgaaaa aataaaagta aaaccactcg
     7561 ctcagacgtg tataaagttg aggagtggtt ttattgtatc aatttttatt acgctataaa
     7621 gattttttaa ctcaatggaa gttatatatt ctaagtgctg ttgttttaat tatggtatta
     7681 attggtttta tattctggag acaagatgat tatacttcaa gaaattttga aaataaagat
     7741 actgctctga aacaaagcac tagtgaaaat agtagtttgt ccaaagtaga agatgtccag
     7801 gtcaaagatg gagataattc caaaaataag ggtcctgtat atgtcgatgt aaaaggtgct
     7861 gttaaacatc ctaatgttta taaaatgaca tctaaggata gagtagttga tttacttgat
     7921 aaagcacaat tattggagga tgcagatgta agtcaaatta atttgtctga aaaattaaca
     7981 gatcaaaaaa tgattttcat acctcataaa ggacaaaaga atgttgaacc acaaattgaa
     8041 gtaaacagtg tgcacgaaaa aaatgggaac acaaataata ctaaagtaaa tttaaatacg
     8101 gcatctgtat cagaattgat gtctgttcct ggagttgggc aagctaaagc taatgcaatt
     8161 gttgaatatc gcaaccaaca aggtgcattt caagaaattg acgatttgaa aaaagtaaaa
     8221 ggttttggaa gtaaaacttt tgataaactg aaatcttatt tcacgacata atggattatt
     8281 ttaattgcaa attttatttt ttgtgattaa tcttatatta ttagaaaatg ttactaaatt
     8341 ttaagtatgg aaggtgaaca tcttggaaag aatcaaatgg gaagaatatt ttatggcaca
     8401 aagtcatttg ctagcattac gttcaacttg tcaaagatta tctgtaggtg caacgattgt
     8461 taaggataat cgtattattg ctggtggtta taatggctct gtagctggcg aggtgcattg
     8521 tatagatgaa ggatgtttaa ttgaagatgg acattgtatc agaacgatac atgcagaaat
     8581 gaatgcttta ttacaatgtg caaaacaagg tgtatctact gaaggtgcaa caatctatgt
     8641 tactcatttt ccatgcctaa attgtacaaa gtcaattatt caagcaggta taaagcgtat
     8701 ctactatgca gaagattatc ataaccatga atatgcaact aaattactca aacaatctgg
     8761 tattgaattt aaaaaaattc cattttcacc agaatatgtt gctaaatatc tgactaaagg
     8821 ttaatgtttt gctgtttgtc gcgttatcaa tgattgtagg agtgctttgg aattctagca
     8881 aagtgctctc tacatttctt ttcattttac ttttgtatat tacttatcgt aaaaataaaa
     8941 tcgtttatgc ccctatttct ctctttttaa tcattttctc cgcatggtat ttacattatt
     9001 cacaacaagc aatatttaat tatatcaatt atattgaacg taattctcag tttaatgagc
     9061 gtgctcaagt aatccaaatt caacgtcaag gtagtgacac atataaaggt aggttgagtt
     9121 taaaaaatga aatatatcct ttctttttaa cagataaaaa gaattttgat ttaaagaaaa
     9181 ttgaaagtcg taattgtatt gttaaaggac aattcaaagt taatgacaat aagtttgtaa
     9241 ctcttaaatt acaaagtata gttgtacaaa gctgcctaga atcgaaccgg tctaatttaa
     9301 ttgagaaaca taaacagttt ataatgaatc gaatttatga ttcgggtatt aagtttccgg
     9361 atcgtattat ggcattgatt actggtgacg taaaagaagt taatgagcaa tttaaggaac
     9421 gtgttaaaga gatagttata tatcatttgc tggcagttag tggctcgcat atagctgcaa
     9481 ttgtattttt aatttaccaa cctttaaaac gattaaattt acctttattt gtcattaaag
     9541 gaattacaat cattgtatta gctttatttg ctcaatacac aaattatgca cctagtgctg
     9601 taagagctat aataatg
//
LOCUS       DAKTIO010000103         9483 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.103, whole genome shotgun sequence.
ACCESSION   DAKTIO010000103 DAKTIO010000000
VERSION     DAKTIO010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9483
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.103"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(219..1511)
                     /gene="asnS"
                     /locus_tag="O0293_001833"
     CDS             complement(219..1511)
                     /gene="asnS"
                     /locus_tag="O0293_001833"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCY0280716.1"
                     /translation="MKTTIKQAKDHLNQDVTIGAWLTNKRSSGKIAFLQLRDGTGFMQ
                     GVVVKSEVDEEVFKLAKEITQESSLYVTGTITEDNRSDLGYEMQVKSIEVISEAHDYP
                     ITPKNHGTEFLMDHRHLWLRSKKQHAVMKIRNEVIRATYEFFNKDGFTKVDPPILTAS
                     APEGTSELFHTKYFDQDAFLSQSGQLYLEAAAMAHGKVFSFGPTFRAEKSKTRRHLIE
                     FWMIEGEMAFTNHAESLEIQEQYVTHVVKSVLENCKLELKILERDTSKLEKVATPFPR
                     ISYDDAIEFLKAEGFDDIEWGEDFGAPHETAIANHYDLPVFITNYPTKIKPFYMQPNP
                     ENEETVLCADLIAPEGYGEIIGGSERVDDLELLEQRVKEHGLDEEAYSYYLDLRRYGS
                     VPHCGFGLGLERTVAWISGVEHVRETAPFPRLLNRLYP"
     gene            complement(1833..4526)
                     /locus_tag="O0293_001834"
     CDS             complement(1833..4526)
                     /locus_tag="O0293_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase DinG"
                     /protein_id="HCY0280717.1"
                     /translation="MGMATYAVVDLETTGNQLDFDDIIQIGITFVRNNQIIDTYHSMI
                     RTNLEIPPFIQALTSIEENMLQQAPYFNQVAQEIYDKIKDCIFVAHNVDFDLNFIKKA
                     FKDCNIQYRPKKVIDTLEIFKIAFPTDKSYQLSELAEAHGITLANAHRADEDAATTAK
                     LMILAFEKFEKLPLDTLKQLYYLSKQLKYDLYDIFFEMVRQYDAKPLDKSYEKFEQII
                     YRKQVDFKKPTTNYNGSLKSLYSKAVDQLGLTYRPQQLYLAETILDQLMHSEKAMIEA
                     SLGSGKSLAYLLAALMYNIETGKHVMISTNTKLLQSQLLEKDIPAMNEALNFKINALL
                     IKSKSDYISLGLISQILKDDTSNYEVNILKMQLLIWITETPSGDIQELNLKGGQKMYF
                     DQKIETYVPARHDVHYYNFIKRNAQNIQIGITNHAHLIHSDVENSIYQLFDDCIVDEA
                     HRLPDYALNQVTNELSYADIKYQLGLIGKNENEKLLKAIDQLEKQRILEKLDIAPIDI
                     FGLKASMNEIHELNEQLFSTIFTIINDSDVYDDDIHRFHNVFTFETKDILKDLHAIID
                     KLNKTLEIFNGISHKTVKSLRKQLLYLKDKFKNIEQSLKAGHTSFISIKNLSQKSTIR
                     LYVKDYAVKDVLTKQVLEKFKSLIFISGTLKFNHSFEAFKQLFNKDVHFNTFEVNTSL
                     QSAKNTSVFIPSDVASYQYKNIDEYVASIVSYIIEYTTITSSKCLVLFTSYKMMHMVQ
                     DMLNELPEFEDYVVLTQQQNQNYKIVQQFNNFDKAILLGTSTFFEGFDFQANGIKCVM
                     IAKLPFMNKHNAKYWLMDSEFTSTFKEYVLPDAVTRFRQGLGRLIRNENDRGIIVSFD
                     DRLINSNYKNFFEQTLENYRQKKGDIQQFGKLLRQIQKKKK"
     gene            complement(4550..5521)
                     /locus_tag="O0293_001835"
     CDS             complement(4550..5521)
                     /locus_tag="O0293_001835"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HCY0280718.1"
                     /translation="MSKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLK
                     LEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSALFDFSEVYDSIDSTQLAAKKSL
                     VGNQSSFFILSDEQTKGRGRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALG
                     IRDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLE
                     NFDESIRHRATSIQLHDKNKLDRYQFLEILLQEIEKRYNQFLTLPFSEIREEYTAASN
                     IWNRTLLFTENDKQFKGQAIDLDYDGYLIVRDEAGESHRLISADIDF"
     gene            complement(5508..6710)
                     /locus_tag="O0293_001836"
     CDS             complement(5508..6710)
                     /locus_tag="O0293_001836"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HCY0280719.1"
                     /translation="MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDI
                     TTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYEVTTFRAEEDYVDHRRPSGVTFV
                     RDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMI
                     RCLRFQSQLSFDIATETFEAMRIQMADIKFLSIERIVIELTKLMRGINVEKSFNHLKS
                     LKAFNYMPYFEHLDMNQINVTEAIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQ
                     YIQIMNALPSIITKEQLKMFVYDYDTHLIKNVMVAADVLKANDIQGHEPLIVNLQTID
                     ETLHRLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILK
                     WVDNHVKI"
     gene            complement(6715..7857)
                     /gene="bshA"
                     /locus_tag="O0293_001837"
     CDS             complement(6715..7857)
                     /gene="bshA"
                     /locus_tag="O0293_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HCY0280720.1"
                     /translation="MKIGITCYPSMGGSGIIATELGIKLAERGHEVHFITSNIPFRIR
                     KPLPNMIFHQVEVNQYAVFQYPPYDITLSTKIAEVIKEYDLDLLHMHYAVPHAICGIL
                     AREMSGKDIKIMTTLHGTDITVLGYDHSLQGAIKFGIEKSDIVTSVSKSLAQETHEII
                     ETNKEIIPIYNFVRENEFPTKHNTALKSQFGIAPDEKVLIHVSNFRQVKRIDTIIETF
                     AKVREKIPSKLILLGDGPELVPMRQLTKELNVEEDVLFLGKQDCVSEFYQLSDLVLLL
                     SEKESFGLTLLEAMKTGVVPIGSNAGGIKEVIKHGETGFVVDVGDCDSASDYAIRLLE
                     DKVLYNKLQKNMLADIAERFGSELITDQYEYYYQKMLNEHNKSKGE"
     gene            complement(8098..8415)
                     /locus_tag="O0293_001838"
     CDS             complement(8098..8415)
                     /locus_tag="O0293_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCY0280721.1"
                     /translation="MKSMVEMQREVDEYIGQFKTGYFSPLANLARLTEEVGELAREIN
                     HTYGEKKKKDSEEANTIKAELGDNLFVLLCLANSMGIDMTESFNETMEKFNTRDKNRF
                     ERK"
     gene            complement(8752..9432)
                     /locus_tag="O0293_001839"
     CDS             complement(8752..9432)
                     /locus_tag="O0293_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HCY0280722.1"
                     /translation="MIIYFVILMVLPLWAQHKVKSNYEKYSQVRSTSGKTGREVALEI
                     LHANGIYDVEVVKGEGFLTDHYDPKKKVVSLSPANYDRPSVAGTAIAAHEVGHAIQDH
                     QGYWFLRFRAALVPVANLGSSLSYMIIMLGIILTAIGSAFGSTALWIGAGLMSLAVLF
                     SIVTLPVEFDASSRAMKQITALNIVNEKEYKHARKVLSAAAMTYVAATAVAVAELVRI
                     ILIARSSD"
BASE COUNT         3003 a         1726 c         1253 g         3501 t
ORIGIN      
        1 aacagtattt tttaacacta gattttaaca atgaacttta tggataatca gatgaactat
       61 ttattttcca aatatagtta ctaaacaaaa gtttgttcat gattaatata agccgttatc
      121 aatcttcatt ttccaagaat aaatcgccca gctattaata taggctggac gattcttttt
      181 attatcataa tatatatttt aagtaacgac cctaaaactt atggatataa acggtttaat
      241 aatcttggga atggcgctgt ttcacgaacg tgttcaacac cagaaatcca tgctactgta
      301 cgctctaaac ctaaaccaaa tccacagtgt ggcacactac cataacgacg taagtctaag
      361 tagtaactat atgcttcttc gtctaatcca tgttctttaa cgcgttgttc taacaattct
      421 aagtcatcca cacgttcaga tccaccaata atttcaccgt atccttcagg tgcaattaag
      481 tctgcacata atacagtttc ttcattttca ggatttggtt gcatatagaa aggcttaatt
      541 ttagttggat aattagtaat aaacaccggt aaatcataat gattagcaat ggctgtttca
      601 tgtggcgcac caaaatcttc gccccattca atatcatcaa agccttctgc ttttaagaat
      661 tcaattgcat catcatatga aattctaggg aatggtgtcg caactttttc aagttttgat
      721 gtatcacgct ctaaaatttt caactctagt ttacaatttt ctaaaactga ttttacaaca
      781 tgtgttacat attgttcttg aatttctaaa ctttcagcat gatttgtgaa agccatttct
      841 ccttcaatca tccagaactc gatcaagtgt ctacgtgttt ttgatttttc agctctgaaa
      901 gttggaccaa atgaaaatac ttttccgtgt gccattgctg cagcttctaa gtataactga
      961 ccactttgag ataaaaacgc atcttgatca aagtatttag tatggaataa ttcacttgta
     1021 ccttctggcg cacttgctgt caaaattggt ggatcaacct ttgtaaatcc atctttgttg
     1081 aaaaattcat acgttgcacg aataacttca tttctaattt tcattacagc atgttgtttt
     1141 ttagaacgta accataaatg acggtgatcc attaagaatt ctgtaccatg atttttaggt
     1201 gtaatcggat agtcatgcgc ttctgaaata acttcaattg atttcacttg catttcgtat
     1261 cctaagtcag aacgattatc ttctgtaatt gtgcctgtaa cgtatagaga tgattcttga
     1321 gtaatttctt tcgcaagttt gaatacctct tcatcaactt ctgatttaac tactacgcct
     1381 tgcataaagc ctgttccatc acgtaattgt aaaaaggcga ttttaccact tgaacgttta
     1441 tttgttaacc aagcaccaat tgtaacgtct tggtttaaat gatctttcgc ttgtttaatc
     1501 gttgttttca taaccattct cctatttatt ttttcgttat acaatactca ttcattttaa
     1561 caaaatccgc tttcaagttc tagaactaga ctaaaagata acgtgtaaat ggtaatgatt
     1621 tacgcacaga tttgaacatt aatttatatt aagacaatac aatcatcagt ttcagacaaa
     1681 atatataata aagcctcaat attattagta tgaaggtcgt tttttgtgct aacattcaag
     1741 tttaattata aaactcaaat tttgacgacc aaacaaaaat tcttgtaaca gaacatagca
     1801 catgtcatgt cacaagaatt ttactttgta tttcactttt tctttttttg aatttgtctt
     1861 aataattttc caaactgttg aatgtcgcct tttttctgac gataattttc aagtgtttgt
     1921 tcaaaaaagt ttttataatt actgtttatg agtcgatcat caaatgaaac tattatgccg
     1981 cgatcatttt catttctaat taatcttcca agtccttgtc taaaacgtgt aactgcatca
     2041 ggtaatacat attccttgaa agttgaagtg aattcagaat ccataagcca atattttgca
     2101 ttatgcttgt tcataaacgg taacttcgct atcatcacac atttaatacc atttgcttga
     2161 aaatcaaaac cttcaaaaaa tgttgacgta ccaagcagta tggccttatc aaaattatta
     2221 aactgttgta ctattttata attttggttc tgctgttgtg ttaatacaac ataatcttca
     2281 aattctggca attcatttag catatcttgt accatatgca tcattttata actcgtaaat
     2341 aagactaaac attttgatga cgttatagtc gtatattcaa taatatagct tacaatcgat
     2401 gctacatact catctatatt tttatattga taagatgcta catcactcgg tataaataca
     2461 cttgtatttt ttgcactttg taacgacgtg ttaacttcaa atgtattaaa gtgaacatct
     2521 ttgttgaata actgtttaaa agcttcaaac gaatgattaa attttaaagt accagatata
     2581 aaaatgagcg atttaaattt ttccaatact tgtttcgtta atacatcttt tacagcgtaa
     2641 tctttcacat ataaacgtat tgttgatttt tgagataaat ttttaatcga aatgaaacta
     2701 gtatgtccag cttttaaact ttgttcgata tttttaaatt tatcttttaa atacaacaac
     2761 tgtttccgta atgatttaac tgttttatga ctaatgccat tgaatatttc tagcgtttta
     2821 tttaacttat cgataatcgc atgtaaatcc ttcaaaatgt cttttgtttc aaaagtaaat
     2881 acattatgga agcgatgaat atcatcatca taaacatcag aatcattgat aatcgtaaat
     2941 atcgttgaga acaattgctc atttaactca tgaatctcat tcatactagc cttcaagcca
     3001 aaaatatcaa ttggtgcaat atctaatttt tccaaaattc gctgcttttc cagttgatca
     3061 attgccttta acaatttttc attttcgttt ttaccaatca aaccaagctg atatttaata
     3121 tcagcataac tcaactcatt tgtcacttga tttaaggcat agtctggtaa gcgatgtgct
     3181 tcatccacta tacaatcatc aaacaattga tatattgaat tttcaacatc agaatgaatt
     3241 aaatgtgcat gatttgtaat accaatttga atgttctgtg catttcgctt aataaaatta
     3301 taataatgaa catcgtgacg tgccggtaca tatgtttcaa ttttctggtc aaaatacatc
     3361 ttttgaccac cttttaaatt taattcctgt atatctccgg acggcgtctc tgtaatccaa
     3421 atcagcaatt gcattttcaa gatattcact tcgtaattac ttgtgtcatc ttttaaaatt
     3481 tgactaataa gccccaatga aatgtaatca cttttacttt taatcaatag tgcattaatt
     3541 ttaaaattca acgcttcatt cattgctgga atatcttttt ctaacaattg actttgcagt
     3601 aatttagtat tggtagaaat catgacatgc ttcccagttt caatattata catcaaggcc
     3661 gcaagtaaat atgctaatga tttaccactg cctagtgatg cttcaatcat tgctttttca
     3721 ctatgcatga gctgatctaa tatagtttcc gctaaatata attgttgcgg tcgatatgtt
     3781 aagccaagtt gatctacagc tttgctatat aaagacttca agctgccatt ataatttgtt
     3841 gtcggctttt taaaatcaac ttgcttacga tagataatct gttcgaactt ttcgtacgat
     3901 ttatccaatg gctttgcatc atattgccta accatctcaa agaaaatatc atacaaatcg
     3961 tatttcaact gtttacttaa ataatataat tgcttcaaag tatctaacgg taacttttca
     4021 aatttttcaa aagctaatat catcaattta gcagtagtag cggcatcttc gtcagctcga
     4081 tgggcatttg ctaaggtaat accatgtgcc tctgctaatt cacttaattg atagctttta
     4141 tctgtaggaa aagctatttt aaagatttct agtgtatcta taactttttt gggacgatat
     4201 tgaatattac aatctttaaa tgccttttta ataaaattca aatcaaaatc tacattatga
     4261 gctacaaaaa tgcaatcttt tatcttatcg tagatttctt gtgcaacttg attaaaatat
     4321 ggcgcttgtt gtagcatatt ttcttcaatg gatgttaacg cttgaatgaa cggcggaatc
     4381 tctaaatttg ttctaatcat agaatgatat gtatcaataa tttggttatt gcgcacaaac
     4441 gttataccaa tttgaatgat atcgtcaaaa tctaattggt tgcctgttgt ttccaaatcc
     4501 acaacggcat aggttgccat acccatagct atctctcctt gctttagtgt taaaaatcta
     4561 tatctgcact aattaaacgg tgtgattcac ccgcttcatc tctaacaatt agatagccat
     4621 cgtaatctaa atcaattgct tgtcctttaa actgtttatc attttctgta aatagcaacg
     4681 ttctattcca aatattagaa gctgcagtat attcttcacg aatttcagaa aaaggtaacg
     4741 ttaaaaattg attatatctt ttttcaattt cttgaagtaa tatctctaaa aattgatatc
     4801 tatctaattt atttttatca tgtaattgta tacttgttgc tctatgtcta atactttcat
     4861 caaagttttc tagttgttgc gtcaaattaa tacctatacc acatattatt gcttctatac
     4921 catcattatt agcaaccatt tcagttaaga aaccacacac tttaccatta tcaatatata
     4981 tatcattcgg ccatttcact ttgacttcat cttgactaaa atgttgaatc gcatctctta
     5041 tccctaatgc aataaataaa ttaaatttag atatcattga gaatgcaacg ttaggtctta
     5101 acacgacaga catccaaagt ccttgccctt ttgaagaact ccaatgtcta ttaaatcgcc
     5161 cacgaccttt cgtttgttca tcactcaaga taaaaaatga agattgattt ccaacaagtg
     5221 actttttcgc agcaagttgt gtagaatcta ttgaatcgta tacttcacta aaatcaaaca
     5281 aagcagaact ttttgtatat tggtctatta taccttgata ccaaatatct gggagctgtt
     5341 gtaataaatg ccctttatga tttactgaat ctattttaca tccctctaac tttaattggt
     5401 caatcacttt ttttactgca gtgcgtgaaa tattaagtga ttccgcaatg ctttgtccag
     5461 atatataatt cggtttattt ttatagagta attgaagtac atcttgacta tattttgaca
     5521 tgattatcca cccatttcaa aatttcagtt tcttcgttgc ttactttacc tgttacaatc
     5581 gctatctcaa tttgtcttag cacatctttt aaccacggac cacttttggc atttaaatgt
     5641 gccataagta caccgccatt aaccatcatg tcttttctat tatgcatagg taaacgatgt
     5701 aatgtttcat caatcgtttg aaggttaacg attaatggtt catgtccttg aatatcattt
     5761 gcctttaaca cgtctgctgc aaccattaca tttttaatga gatgcgtatc ataatcataa
     5821 acaaacattt ttaattgttc ttttgtaata atacttggta atgcattcat aatttgaata
     5881 tattgattga tatcttttac ttgtcggtta cttagcttta aaggcttcaa tgagtaatta
     5941 atatcaaatt taactgatac tatagcaatc aacaattcta aatcaattgc ttcagttaca
     6001 ttaatttgat tcatatcaag atgttcgaaa tacggcatat aattaaatgc tttcagcgat
     6061 tttaaatgat taaaactctt ttcaacatta atacctcgca ttaatttagt tagttcaatc
     6121 actatacgct caattgataa aaatttaata tctgccattt gtatacgcat cgcttcgaat
     6181 gtttccgttg caatatcaaa tgataattgt gactggaacc ttaaacatcg aatcatacgt
     6241 aaagcatctt cttggaatcg ttcctcagct atacctacag ttcttattat tcgattatta
     6301 atatcttgtt gaccatcaaa ataatcatac aatttgtatg ctgtatccat tgctatcgca
     6361 ttcatcgtga aatctcgtcg ttgcaaatct tcgtataaat cacgaacaaa tgtaacacca
     6421 cttggtctac ggtgatcgac ataatcttct tcagcccgga atgttgtcac ttcataattt
     6481 tcatcattaa aaactacatt tatcgtgcca tgttctttac ctacaggtat cgtatgacta
     6541 aagatagatt ctatttcatc cggcgttgca cttgttgtga tatctatatc atgaatattt
     6601 cttcccatga cataatctct tacagagcca cctacataat atgcttcaaa accattgtct
     6661 tgaatttgtt ctaatatagg ccttgcctgt tcaaataatg atttatccat attattactc
     6721 gcctttactt ttgttatgct catttagcat tttttgataa taatactcat attgatctgt
     6781 aataagttct gatccaaaac gttcagcaat atctgctagc atgtttttct gaagtttgtt
     6841 gtataacacc ttatcttcaa gtaatcggat agcatagtca ctcgctgaat cacaatcacc
     6901 cacatctacg acaaatccag tttcaccatg tttaataacc tctttaattc caccggcatt
     6961 tgaaccaatt ggaacgacgc ctgttttcat agcctcaagt aaagttagtc caaagctttc
     7021 tttttcactt aataataata ctaagtcaga taattggtaa aattcactta cgcaatcttg
     7081 tttccctaaa aataaaacat cctcttctac gtttaactct ttcgtcaatt gacgcattgg
     7141 cactaattca ggaccatctc caagtaaaat taatttacta ggtatctttt cacgtacttt
     7201 tgcaaatgtt tctataatag tatctatgcg ttttacttgt ctaaaattcg atacatgtat
     7261 taacactttt tcatctggtg ctataccaaa ttgtgatttt aatgctgtgt tatgtttagt
     7321 tggaaactca ttttcacgta caaaattata aatcggtata atttctttgt tagtttcaat
     7381 aatttcatgt gtttcttgtg ctaaagattt actcacactt gtcacaatat cacttttttc
     7441 aatgccaaat ttaattgcac cttggagtga atgatcatag cccaaaacag taatatcagt
     7501 accgtgtagc gttgtcataa tttttatatc tttacctgac atctcacgag ctaaaatccc
     7561 acaaattgca tgaggtacag catagtgcat atgcaacaaa tcaagatcat attctttaat
     7621 aacttcagcg attttagtac ttaacgtaat atcatacggt ggatactgaa atactgcata
     7681 ttgattcact tcaacttgat gaaaaatcat attcggtaat ggttttctta ttctaaacgg
     7741 gatatttgaa gtgataaaat gtacttcgtg acctcgctct gctaatttaa ttcctaattc
     7801 tgtggcaata attccagaac cacccatgga cgggtaacat gttataccta tcttcattcg
     7861 cttgcccatc ctttctttct atttctctct attattctcg atgcgtagat aattgtttaa
     7921 atttaagttt atagtaatgt tgagtttata atttcatata tctaaaaaca ggtgttgtat
     7981 atataatcat tcatctagtt atacttactt taaaaataat ataatttcat gcgatgcaat
     8041 tcattgatgg atgtttttaa tcttaatcaa atccaaataa agcatatatt tttaaattca
     8101 ctttctttcg aatcgatttt tatctcttgt attaaacttt tccattgttt cattaaagct
     8161 ctctgtcata tctattccca ttgaattcgc taaacataac aacacaaata aattatcacc
     8221 taattctgct ttaatcgtat ttgcttcctc tgaatctttc ttcttttttt caccataggt
     8281 atgatttatt tcacgtgcaa gttcgcccac ttcttcagtc aatctagcta agttagctaa
     8341 tggtgaaaaa tatcctgttt taaattgtcc aatatattca tcaacttcac gttgcatttc
     8401 taccattgat ttcatttcta cgttctcctt atattgcatt tctaatatag tatatatcaa
     8461 tttgaagtct catgcatgtt tagtgcaatc tactgactct agctaaacta gatgacaagt
     8521 tgtttatttt agaagtttaa taattcagtt tatataaatg taatgcattc ctaactaaat
     8581 taaatcaatt gaaattggga ttataacttt atgatacgta ccactacaat aaaataatat
     8641 agtgaataat ctaccattag aaaaataagc acaaaaaaac tagcaaccac acaaaaatgt
     8701 gattagctag ttaataagtg tctaatttaa gttaattgtt aatctataag attaatcact
     8761 tgaacgcgca atcaaaataa tacgtacaag ctctgctaca gcgactgcag ttgctgcaac
     8821 ataagtcatt gctgctgcag ataatacttt acgcgcatgc ttgtattctt tttcatttac
     8881 aatgttcaat gccgtaattt gtttcatcgc tcttgaactc gcatcaaact caactggtaa
     8941 cgtaacaatt gagaataata ccgctaatga cattaaacca gcaccaatcc ataaagcagt
     9001 tgaaccaaat gcactaccta tcgctgttaa gataatacct aacatgatga tcatataact
     9061 taatgaactc cctaggtttg caacaggtac taatgctgct ctgaatctta agaaccaata
     9121 tccttggtga tcttgaatgg catgaccaac ttcgtgggct gcaattgcag ttccagcaac
     9181 tgatggtctg tcatagtttg caggagatag tgaaacaact ttctttttag gatcgtaatg
     9241 atctgttaag aatccttcac ctttaacaac ttcgacatca taaataccgt ttgcatgtaa
     9301 aatttctaat gcaacttcac gacccgtttt accactagtt gatctaactt gtgaatattt
     9361 ctcatagtta gatttaactt tgtgttgtgc ccataaagga agcaccatta atattacgaa
     9421 ataaattatc atagtaaaaa ttgaagacaa taaactcact ctcctttata aatattttac
     9481 tgt
//
LOCUS       DAKTIO010000104         9348 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.104, whole genome shotgun sequence.
ACCESSION   DAKTIO010000104 DAKTIO010000000
VERSION     DAKTIO010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9348
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.104"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(262..594)
                     /locus_tag="O0293_001840"
     CDS             complement(262..594)
                     /locus_tag="O0293_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HCY0280723.1"
                     /translation="MGKKMGLGLSIALVVIGIAVVCLMIFSSQKTTYFGYMNSNTNAE
                     KVVSEKDGLVKHNIKVEPSNDFKPKKGDFVKLVSKDDGKTFYKQEIVKHDDVPHGLMM
                     KIHDMHMN"
     gene            complement(678..1823)
                     /locus_tag="O0293_001841"
     CDS             complement(678..1823)
                     /locus_tag="O0293_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HCY0280724.1"
                     /translation="MFQLLAVCPMGLEAVVAREIQELGYETNVENGRIFFEGDASAIV
                     KANLWLRTADRIKIVVGRFNATTFDELFEQTKALPWESIIDKEGNFPVQGRSVKSTLH
                     SVPDCQAITKKAIVERLRRAYNEKGWLNESGAKYPVEVAILKDNVLLTIDTSGSGLNR
                     RGYRLAQGEAPIKETLAASLIRLANWKGDTPLIDPFCGSGTIAIEACLIAQNIAPGFN
                     REFVSEQWNIMPANIYDDYRDEADKMADYDKEIEVYASDIDPEMVEIAKRNAEEVGLS
                     DIIKFSVKDVNTLTIDTEEPVALIGNPPYGERIGDREEVEEMYRYIGKLMKQHPFLST
                     YILTSNKEFEYLVDRKATKRRKLFNGYIECTYYQYWGKKTERKTIEN"
     gene            complement(2032..2411)
                     /gene="rnpB"
                     /locus_tag="O0293_001842"
     ncRNA           complement(2032..2411)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="O0293_001842"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(2474..2818)
                     /gene="gpsB"
                     /locus_tag="O0293_001843"
     CDS             complement(2474..2818)
                     /gene="gpsB"
                     /locus_tag="O0293_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HCY0280725.1"
                     /translation="MSDVSLKLSAKDIYEKDFEKTMARGYRREEVDAFLDDIIADYQK
                     MADMNNEVVKLSEENHKLKKELEELRLRVATSRPQDNKSFSSNNTTTNTSSNNVDILK
                     RISNLEKAVFGK"
     gene            complement(2832..3395)
                     /locus_tag="O0293_001844"
     CDS             complement(2832..3395)
                     /locus_tag="O0293_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HCY0280726.1"
                     /translation="MVKTVYVTGYKSFELNIFKDDAPEVHYLKQFIKHKIEQLLDEGL
                     EWVLIQGQMGIELWTAEVVIELQRTYDSLKFAVITPFQGHTEKWNEHNQSKYANIIKH
                     ADYVDSIFHTSYQGPFQFKQADQFMLEHSDQTLLIYDEEQEASPKFFKQMLVDFMDKT
                     NYTCDIVTFDELTAFINDLQWSEDQSF"
     gene            complement(3388..3738)
                     /locus_tag="O0293_001845"
     CDS             complement(3388..3738)
                     /locus_tag="O0293_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YppE family protein"
                     /protein_id="HCY0280727.1"
                     /translation="MNDVVESLIYEVNNMQQNFENVKSQQQDHDFYQTVKPYTEHIDS
                     ILNEIKLHREFIIEVPYMNSRKFELLIANIEQLSVECHFKRTSRKLFIEKLKSVQYDL
                     QNILDGVTKEGTDG"
     gene            complement(3793..3972)
                     /locus_tag="O0293_001846"
     CDS             complement(3793..3972)
                     /locus_tag="O0293_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001788819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280728.1"
                     /translation="MLLISRGVFDLNDFIRLKVFSSYNNIHDKILYLYRKFLDLLDLT
                     DNTFEKIVKSNYIEC"
     gene            4227..4904
                     /gene="recU"
                     /locus_tag="O0293_001847"
     CDS             4227..4904
                     /gene="recU"
                     /locus_tag="O0293_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HCY0280729.1"
                     /translation="MLCIINNVYYQTCVVKFMNYPNGKPYRKNSAIDGGKKTAAFSNI
                     EYGGRGMSLEKDIEHSNTFYLKSDIAVIHKKPTPVQIVNVNYPKRSKAVINEAYFRTP
                     STTDYNGVYQGYYIDFEAKETKNKTSFPLNNIHDHQVEHMKNAYQQKGIVFLMIRFKT
                     LDEVYLLPYSKFEVFWKRYKDNIKKSITVDEIRKNGYHIPYQYQPRLDYLKAVDKLIL
                     DESEDRV"
     gene            4901..7084
                     /locus_tag="O0293_001848"
     CDS             4901..7084
                     /locus_tag="O0293_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCY0280730.1"
                     /translation="MTENKGSSQPKKNGNNGGKSNSKKNRNVKRTIIKIIGFMIIAFF
                     VVLLLGILLFAYYAWKAPAFTEAKLQDPIPAKIYDKNGELVKTLDNGQRHEHVNLKDV
                     PKSMKDAVLATEDNRFYEHGALDYKRLFGAIGKNLTGGFGSEGASTLTQQVVKDAFLS
                     QHKSIGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYYSDGVTGIKAAAKYYFNKDLKD
                     LNLAEEAYLAGLPQVPNNYNIYDHPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDL
                     KANLVNRTPEERQNIDTNQDSEYNSYVNFVKSELMNNKAFKDENLGNVLQSGIKIYTN
                     MDKDVQKTLQNDVDNGSFYKNKDQQVGATILDSKTGGLVAISGGRDFKDVVNRNQATD
                     PHPTGSSLKPFLAYGPAIENMKWATNHAIQDESSYQVDGSTFRNYDTKSHGTVSIYDA
                     LRQSFNIPALKAWQSVKQNAGNDAPKKFAAKLGLNYEGDIGPSEVLGGSASEFSPTQL
                     ASAFAAIANGGTYNNAHSIQKVVTRDGETIEYDHTSHKAMSDYTAYMLAEMLKGTFKP
                     YGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLPDNAAKDVWINGFTPQYTMSVWMGF
                     SKVKQYGENSFVGHSQQEYPQFLYENVMSKISSRDGEDFKRPSSVSGSIPSINVSGSQ
                     DNNTTNRSTHGGSDTSANSSGTAQSNNNTRSQQSRNSGGLTGIFN"
     gene            complement(7650..7991)
                     /locus_tag="O0293_001849"
     CDS             complement(7650..7991)
                     /locus_tag="O0293_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280731.1"
                     /translation="MTNITKEVFDNLEQEIDLFAKNKTLGSSEAKPYLDEYHSKIIDY
                     FKQVNDITGNIDFDKLNQYPVVPMNFKERYDYMIERKYHFMGYRQMKTFKTELIKMNA
                     SYQTRLKNKQV"
     gene            complement(7996..8655)
                     /gene="nth"
                     /locus_tag="O0293_001850"
     CDS             complement(7996..8655)
                     /gene="nth"
                     /locus_tag="O0293_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCY0280732.1"
                     /translation="MVSKKKALEMIDVIANMFPDAECELKHDNPFELTIAVLLSAQCT
                     DVLVNRVTTELFKKYKTPEDYLAVSDEELMNDIRSIGLYRNKAKNIKKLCQSLIDQFN
                     GEIPQTHKELESLAGVGRKTANVVMSVAFDEPSLAVDTHVERVSKRLGINRWKDNVRQ
                     VEDRLCSVIPRDRWNRSHHQLIFFGRYHCLARKPKCDICPLLEDCREGQKRYKASLKE
                     A"
     gene            complement(8645..9331)
                     /locus_tag="O0293_001851"
     CDS             complement(8645..9331)
                     /locus_tag="O0293_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HCY0280733.1"
                     /translation="MDKYQLKARPVVIRRELLDHYSDLGLDEQDLVILLKLIYASETS
                     NKQPSIELLQKGSTMQPRDITMVIQNLIQRELLELQVQKDEEGRFTEYMNLDPFFEKL
                     SHILKQQSMETKELNSKEKFKQLFRVLEDTFARPLSPYEIETLNQWIDVDKHDTAIIQ
                     AALDEANSLNKLSFKYIDRILLNWKKNNVKTIDDSRKIREKFNKPKMTHTVKTVPKFD
                     WLNGENLDGK"
BASE COUNT         3044 a         1641 c         1358 g         3305 t
ORIGIN      
        1 ttgtatttat aattgaatta atattatata ttaaagatcg tcgtgctaaa caataatgca
       61 cttaaagttt tgaactgacg aaatttacaa aatgattctc gtctctctaa ttacttaaaa
      121 cgggttctat aataaatcgt actgatggaa agtttttact ttttatctgt ccgatttttt
      181 gaatttgaag ataaaaaaac atctaaaacg cataagaaga taactctact tcttcaaaat
      241 tacgttatag atgctttttt attaattcat atgcatgtcg tgaattttca tcattaaacc
      301 gtgtgggacg tcatcatgtt taacaatctc ttgtttataa aatgtcttcc catcatcttt
      361 agaaactaat tttacaaagt ctcctttttt cggcttgaaa tcattagatg gttctacttt
      421 gatattatgt ttgactaatc catctttttc actgacaact ttttctgcat ttgtattact
      481 attcatataa ccaaaataag tcgttttttg actagaaaaa atcattaaac atacaacggc
      541 aataccaata acaaccaatg caatagataa acctagaccc atttttttac ccatttatag
      601 actccttcat ttttcaaaaa tacctattat catgagaact taattattac taattttagt
      661 cacgcattag aaacacttta gttttcaata gttttacgtt ctgtcttttt accccagtat
      721 tgataataag tacattctat atatccatta aataacttac gtcgctttgt tgctttacga
      781 tctactaaat attcaaattc tttattactt gttaaaatgt atgtagataa aaatggatgt
      841 tgtttcatta gtttaccaat ataacggtac atttcttcaa cttcttcacg atcaccaata
      901 cgttcaccat atggaggatt tccaattaac gccaccggtt cttctgtatc aattgttaat
      961 gtattgacat cttttacact aaatttaata atatcagaca atccaacttc ttcagcgtta
     1021 cgcttagcaa tctctaccat ttctggatcg atatcagaag catatacttc gatttcttta
     1081 tcataatcag ccatcttatc cgcttcatca cggtaatcat cataaatatt tgctggcatg
     1141 atgttccatt gctctgatac gaactcgcga ttaaaaccag gtgcgatatt ttgagcaatt
     1201 aaacaagctt ctatagctat tgttcccgaa ccgcaaaatg gatcaattaa aggtgtatca
     1261 cctttccagt ttgcaagacg gattaaactt gctgccaacg tttctttaat tggtgcttca
     1321 ccttgtgcta atctataacc acgtctgttc aaaccagaac ctgatgtgtc gatagtcaat
     1381 aatacattat cttttaaaat ggcaacttca acagggtatt tggcacctga ttcatttaac
     1441 caaccttttt cgttatatgc gcgacgtaat cgttcaacaa tagctttctt agttatcgcc
     1501 tgacaatctg gcacactatg tagtgttgat ttaacgcttc taccttgaac tgggaagtta
     1561 ccctctttat caattataga ttcccaaggg agcgctttgg tttgttcgaa taattcgtca
     1621 aacgttgttg cgttaaaacg tccaacaaca attttgattc ggtctgctgt gcgcaaccat
     1681 aaatttgcct ttacaattgc acttgcgtct ccttcaaaaa atatacgacc attttcaaca
     1741 tttgtttcat agcctaattc ttgaatttcc ctagcaacaa cagcttctaa tcccatcgga
     1801 caaactgcaa gtaattgaaa catatatgat tctcctttta tacaggtatt ttattcttag
     1861 cttgtgtttt ttatacattt ccaacaaatt taatcgctga tacattaacg catccgctta
     1921 ctattttaaa acaaggcagt gtcattatat caagacaagg cgttaatttt aagtgttctt
     1981 ttcatgaaaa aagctctcca tcatctagga gagctaaact agtagtgata tttctataag
     2041 ccatgttctg ttccatcgta ctcatcacgt gcactagtca cactggtact caggtgataa
     2101 ccatctgtct acaccacttc atttcgcgaa gtgtgtctcg tttatacgtt gaattccgtt
     2161 aaacaagtgc tcctaccaaa tttggattgc tcactcgagg ggtttaccgc gttccacctt
     2221 ttatatttct ataaaagcta cgtcactgtg gcactttcaa attactctat ccatatcgaa
     2281 agacttagga tatttcattg ccgtcaaatt aatgccttga tttattgttt catcaagcac
     2341 gaacactaca atcatctcag actgtgtgag catggacttt cctctatata atatagcgat
     2401 tacccaaaat atcactttta aaattataac atagtcatta ttagtaagac agttaaactt
     2461 ttgtatttag taattattta ccaaatacag ctttttctaa gtttgaaata cgttttaaaa
     2521 tatctacatt atttgaagat gtatttgttg ttgtattatt cgaagaaaaa cttttattgt
     2581 cctgaggtct tgatgttgct acacgtagtc ttaattcttc taattctttt ttaagtttat
     2641 gattctcttc tgataatttt acaacttcat tattcatatc ggccattttt tgataatcag
     2701 caataatgtc atctaaaaat gcatctactt cttctcttct atagccacga gccatcgttt
     2761 tttcaaaatc tttttcataa atatcttttg ctgataattt caatgaaaca tctgacattt
     2821 tttccacctc attagaaact ttgatcttca gaccactgta agtcattgat gaatgctgtt
     2881 aattcatcga acgtcacaat atcacaagta tagtttgttt tatccataaa atcaactaac
     2941 atctgcttga agaacttagg actcgcctct tgttcctcat cataaatgag caatgtttga
     3001 tccgaatgtt caagcataaa ttgatctgct tgtttaaatt gaaaaggacc ttgatacgac
     3061 gtatgaaaaa tactatcaac ataatctgca tgcttaatta tgttggcata cttactttga
     3121 ttatgttcat tccacttttc tgtatgtcct tggaacggtg taattacagc aaactttaac
     3181 gaatcatatg ttcgttgtaa ttcaataaca acttctgcag tccataattc tatacccatt
     3241 tgcccttgta ttaacaccca ttctaatcct tcatccaaca gttgttcaat tttatgtttt
     3301 ataaattgtt ttaaataatg tacttcaggt gcgtcatctt taaaaatgtt taattcgaat
     3361 gatttgtaac ctgttacata aactgtttta accatcagta ccctcttttg ttacgccatc
     3421 taatatattt tgtaaatcat attgaacact tttaagcttt tctataaata actttcgact
     3481 tgttcgctta aaatgacatt cgacagaaag ttgttcaatg ttagcaatca gtagctcaaa
     3541 tttccttgaa ttcatataag gtacttctat aataaattca cgatgtaatt tgatctcatt
     3601 gagtatgcta tcaatatgtt cagtatatgg ctttacagtt tggtaaaaat catgatcttg
     3661 ttgttgtgat ttcacatttt caaaattttg ttgcatgttg ttaacttcat aaattagtga
     3721 ttcgactaca tcattcatga ggacgcctcc tacatttttt aatttatcac aatatactgt
     3781 attcgtcatg ttttaacact ctatataatt tgatttaact attttttcaa atgtgttatc
     3841 tgttaaatca agtaaatcta aaaacttcct atataaatac aaaattttat cgtgtatgtt
     3901 gttatacgat gaaaatactt ttaatctaat aaaatcattt aaatcaaata cacctctgct
     3961 gattaacaac acatacttgt acttgcctca aaaataaaaa ttactaatca tgatttgact
     4021 tttataacaa aattcaaaaa tattgtaatg agtattcatt tttataagca aaacttttca
     4081 attaattaaa attgtgtgta aaattccaac attaacggta aatgtattat aatattattg
     4141 tgttaatgct gaaatcacgt taaaataagc tttggtgtat tgtgtctttc gcatttatac
     4201 taatttaagt ttggtgtttc actataatgt tgtgtataat aaacaacgtt tactaccaaa
     4261 cttgtgtggt gaaatttatg aattatccaa atggtaaacc atatcgtaaa aatagtgcta
     4321 tagacggagg gaaaaagacc gctgccttta gtaatattga gtatggtgga cgtggtatgt
     4381 cacttgaaaa agatatcgaa cattcaaata cgttttatct taaaagcgac attgcagtta
     4441 ttcacaaaaa gcctacgcca gtacaaatag ttaatgtcaa ctatcctaag cggagtaaag
     4501 ctgtgattaa cgaagcttat tttcgtacac cttcaacaac tgattacaac ggcgtttatc
     4561 aaggttatta tattgatttt gaagcaaagg aaactaaaaa caagacgtcc tttcctttaa
     4621 ataatattca tgaccatcaa gtcgaacata tgaaaaatgc atatcaacaa aaaggtattg
     4681 tgtttttaat gattcgtttt aaaacgctag atgaagttta tcttttaccc tattcaaaat
     4741 tcgaagtatt ttggaagaga tataaagata atattaaaaa gtctataaca gttgatgaaa
     4801 tacgaaaaaa tggttaccat attccttatc agtatcaacc aagattagac tatctaaaag
     4861 cagttgataa gttgatatta gatgaaagtg aggaccgcgt atgacggaaa acaaaggatc
     4921 ttctcagcct aagaaaaacg gtaataatgg tgggaaatcc aactcaaaaa agaatagaaa
     4981 tgtgaagaga acgattatta agattattgg cttcatgatt attgcatttt tcgttgttct
     5041 tttactaggt atcttattgt ttgcttatta tgcttggaaa gcacctgctt ttaccgaagc
     5101 taaattacaa gatccgattc ctgcaaagat atatgacaag aacggagaac ttgttaaaac
     5161 attagataat ggccaaagac atgagcatgt aaatttaaaa gacgtgccga aatcaatgaa
     5221 agacgcagta cttgcaactg aagacaatcg tttctacgaa catggcgcac ttgattataa
     5281 acgtttattc ggtgcaattg gtaagaactt gactggtgga tttggttctg aaggtgcctc
     5341 aacattaaca caacaagttg ttaaagatgc atttttatca caacataaat ctattggacg
     5401 taaagctcaa gaagcatact tatcatatcg tttagaacaa gagtatagta aagatgatat
     5461 cttccaagta tatctaaaca aaatttacta ttctgatggc gtaacaggta ttaaagctgc
     5521 tgctaagtat tactttaata aagatttaaa agatttaaac ttagcggaag aagcttattt
     5581 agccggttta cctcaggttc caaacaacta taatatttat gatcatccaa aagctgctga
     5641 agatcgtaaa aacactgttt tatacttaat gcattatcat aaacgcatta cagataaaca
     5701 gtgggaagat gctaagaaaa tcgatttaaa agcgaactta gtaaatcgta ctcctgaaga
     5761 acgtcaaaac attgatacaa atcaagattc tgagtataat tcatacgtta actttgtaaa
     5821 atctgaatta atgaataata aagcattcaa agatgaaaat ttaggtaatg tattacaaag
     5881 tggtattaaa atttatacaa acatggataa agatgttcaa aaaacattac aaaatgatgt
     5941 tgataatggt agcttctaca agaataaaga ccaacaagtt ggtgcaacaa ttcttgatag
     6001 taaaactggt ggtttagttg ctatatctgg tggacgtgat ttcaaagacg tcgttaacag
     6061 aaaccaagca acagatcctc accctactgg ttcatcttta aaacctttct tagcgtatgg
     6121 acctgccatt gaaaatatga aatgggcaac aaaccatgcg attcaagatg aatcttcata
     6181 tcaagttgat ggttctacat ttagaaacta tgatacgaag agtcacggta ctgtatctat
     6241 ttatgatgct ttacgacaaa gtttcaatat cccagcttta aaagcttggc aatcagttaa
     6301 gcaaaatgct ggtaatgatg cacctaagaa attcgctgcc aaacttggct taaactacga
     6361 aggcgatatt ggtccatctg aagtacttgg tggttctgct tcagaattct caccaacaca
     6421 attagcatca gcatttgctg caatcgctaa cggtggtact tataacaacg cgcattcaat
     6481 tcaaaaagta gttactcgtg atggtgaaac aatcgaatac gatcatacta gccataaagc
     6541 gatgagtgat tacactgcat acatgttagc tgagatgcta aaaggtacat ttaaaccata
     6601 tggttctgca tatggccatg gtgtatctgg agtaaatatg ggtgctaaga caggtactgg
     6661 tacttacggt gctgaaactt attcacaata taatttacct gataatgcag cgaaagacgt
     6721 gtggattaac ggctttacac ctcaatacac tatgtcagtg tggatgggct tcagtaaagt
     6781 taaacaatat ggtgaaaact catttgtggg acatagccaa caagaatatc cacagttctt
     6841 atatgaaaat gtgatgtcaa aaatttcatc tagagatggc gaagacttta aacgtcctag
     6901 ctcagtaagt ggtagtatcc catcaatcaa tgtttctggt agtcaagata acaacactac
     6961 aaatcgtagt acacacggtg gtagtgacac atcagcaaac agcagtggta ctgcacaatc
     7021 aaataacaat actagatctc aacaatctag aaacagcggt ggattaacag gtatattcaa
     7081 ctaatccact caacataaaa tcctcagtta taccatattt atggtgtagc cgaggatttt
     7141 tttaggttct tcatctttta tggtgggaag gtaaaacttc ctgctttttt tataagtatt
     7201 catacgctat tgctatatta gtagaaaccg taaaatcagt cattcacata cattttgaaa
     7261 catctaaata gtttttcata ttcacatctt tagctatgta aaattattga atttgaataa
     7321 gtggttgttc ccatttttaa aattatgatt atattttctc atagtcatct tagacgctgt
     7381 tttaaattga tatatttatt aattccctgt gtacgttaat cagtgaaata tcaacatgtt
     7441 tgcaacatat tttcacaatc atagatataa gaaatccacc tacgattcga aatagatatc
     7501 ttaaagcata tctcaagata tccattatga tgtagatgga cagtttttat attaagacgt
     7561 tgtaatgaca cgacacctaa cataattata aatatgtgtg catgaattag ttttatatat
     7621 tttcaacatt aggaaatgat caatcttgtc tatacctgct tattctttaa ccttgtttga
     7681 taactagcat tcattttaat caattcagtt ttaaaggtct tcatttgtcg atagcccata
     7741 aaatgatatt tacgttcaat catataatca tatctttctt taaaattcat tggcacaaca
     7801 ggatattgat ttaatttatc aaaatcaata ttgccagtta tgtcattaac ctgcttaaaa
     7861 taatcaataa ttttactatg gtattcatcc aagtagggct ttgcttcact agaacctaat
     7921 gtcttatttt tggcaaataa atcaatctct tgttctaaat tatcaaatac ctcttttgta
     7981 atatttgtca tgttatcacg cttctttcaa actagcttta taacgttttt gtccttctct
     8041 acaatcttct aatagtggac aaatatcgca tttaggtttt cgggctaaac agtggtatct
     8101 tccaaagaaa atgagttgat gatggctcct attccatcta tctctaggta tgacagaaca
     8161 taaacggtct tctacctgtc tcacattatc tttccaacga ttaataccta agcgtttaga
     8221 aacacgttct acatgcgtat caacagctaa tgaaggttca tcaaatgcta cactcatgac
     8281 tacattagca gttttacgtc ctacacctgc taaactttct aattccttat gtgtttgtgg
     8341 tatttctcca ttaaattgat caatcaaaga ttgacaaagt ttcttaatat tcttagcttt
     8401 gttacgatac agaccgatag aacgaatatc attcataagt tcttcatcac tgactgccaa
     8461 ataatcttca ggcgttttgt attttttaaa cagctcagtt gttactctat ttactagaac
     8521 gtctgtacat tgcgctgaca ataatacagc aatagttaat tcgaacggat tatcatgttt
     8581 taattcacat tctgcatccg gaaacatatt tgctataaca tcaatcattt ctaatgcttt
     8641 tttcttactt accatcaagg ttctccccgt ttaaccaatc aaatttaggt accgttttaa
     8701 ctgtgtgcgt cattttcggt ttattgaatt tttctcttat ttttctagaa tcgtcaattg
     8761 ttttgacatt gtttttcttc caattaagta aaatacgatc tatatattta aagctaagtt
     8821 tattcaaact attcgcctcg tctaatgccg cttgtataat tgcagtatcg tgtttatcaa
     8881 catcaatcca ttgatttaac gtttctattt catatggaga taacggcctt gcaaatgtat
     8941 cctctaaaac tctaaataat tgtttaaatt tttctttact atttagctct ttcgtttcca
     9001 tactttgttg cttcaatata tgacttaatt tttcgaaaaa aggatctaga ttcatatatt
     9061 cggtaaatct accttcttca tctttttgaa cttgtaattc tagcaattca cgttgtatca
     9121 aattttgaat aaccattgta atatcacgtg gttgcatagt tgagcccttc tgaagcaatt
     9181 caattgaagg ctgtttattt gatgtttcgg aagcataaat caatttaagc aaaatgacta
     9241 aatcttgctc atctaaacct aagtcactgt aatggtctaa taattctctt cgtatcacta
     9301 caggtcttgc ttttaattga tatttatcca tgttcgtgcc ccctttta
//
LOCUS       DAKTIO010000105         9331 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.105, whole genome shotgun sequence.
ACCESSION   DAKTIO010000105 DAKTIO010000000
VERSION     DAKTIO010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9331
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.105"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            659..841
                     /locus_tag="O0293_001852"
     CDS             659..841
                     /locus_tag="O0293_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280734.1"
                     /translation="MKDSIFWKKAFIPVYFIVAMLVFLLFRFYIKTDNFSIYLMSIFL
                     ICLGTASIIYNYKTNR"
     gene            complement(823..1062)
                     /locus_tag="O0293_001853"
     CDS             complement(823..1062)
                     /locus_tag="O0293_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280735.1"
                     /translation="MCVVIMRDVGLIDVEDCYDIFGMWCLVNGGLKLSFYGGLKMLFY
                     VGLEFVVLWRYKKPQQDLDLTVTYTFHTYIYRLVL"
     gene            1295..2959
                     /gene="alsS"
                     /locus_tag="O0293_001854"
     CDS             1295..2959
                     /gene="alsS"
                     /locus_tag="O0293_001854"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HCY0280736.1"
                     /translation="MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGP
                     ELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGG
                     QVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFI
                     SIPQDVISSPVESKAISLCQKPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSAEE
                     TNAIRKLVERTNLPVVETFQGAGVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTI
                     GYDPIEYEASNWNKELETQIINIDEVQAEITNYMQPKKELIGNIAKTIEMISEKVDEP
                     FINQQHLDELEQLRTHIDEETGIKATHEEGILHPVEIIESMQKVLTDDTTVTVDVGSH
                     YIWMARNFRSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQ
                     DLETAVRKNLNIIQLIWNDGKYNMVEFQEEMKYKRSSGVDFGPVDFVKYAESFGAKGL
                     RVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN"
     gene            2996..3700
                     /gene="budA"
                     /locus_tag="O0293_001855"
     CDS             2996..3700
                     /gene="budA"
                     /locus_tag="O0293_001855"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCY0280737.1"
                     /translation="MTNVLYQHGTLGTLMAGLLKGTASINELLQHGDLGIATLTGSNG
                     EVIFLDGKAYHANEHKEFVELKGDELTPYATVTKFVADTSYETKDKSSEAVFAEIKEK
                     MLSENLFSAVKISGLFKKMHVRMMPAQEPPYTRLIDSARRQPEQTETYVKGSVVGFFT
                     PELFHGIGSAGFHVHFANDDRNFGGHVLDFEVEDVKVEIQNIETFEQHFPIQDKDFTK
                     ANIDYKDIADEIREAE"
     gene            complement(4064..4489)
                     /locus_tag="O0293_001856"
     CDS             complement(4064..4489)
                     /locus_tag="O0293_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCY0280738.1"
                     /translation="MKLKSFVTATLALGLLSTVGAALPSHEASADSNNGYKEMTVDGY
                     HTVPYTISVDGITALHRTYFIFPENKNVLYQEIDSKVKNELASQRGVTTEKINNAQTA
                     TYTLTLNDGNKKVVNLKKNDDAKNSIDPSTIKQIQIVVK"
     gene            4783..5598
                     /locus_tag="O0293_001857"
     CDS             4783..5598
                     /locus_tag="O0293_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HCY0280739.1"
                     /translation="MSKRLLLFDFDETYFKHNTNEEDLSHLREMEKLLEKLTNNNEVI
                     TAVLTGSTFQSVMDKMDQVNMTFKPLHIFSDLSSKMFTWNNGEYVESETYKKKVLSEP
                     FLFEDIEDILRHISAQYNVEFIPQRAFEGNETHYNFYFHSTGNHNNDSRILEALVRYA
                     NDQNYTARFSRSNPLAGDPENAYDIDFTPSNAGKLYATQFLMRKYNIPVKSILGFGDS
                     GNDEAYLSYLEHAYLMSNSRDEALKQKFRLTKYPYYQGITLHVKEFVEGKYDY"
     gene            5809..6663
                     /locus_tag="O0293_001858"
     CDS             5809..6663
                     /locus_tag="O0293_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HCY0280740.1"
                     /translation="MNKTNFGAYLNKSKSRKIYKIFDQNLKREFSYWQFRKLFKSYTR
                     QSDVHELKVCTQNDGTIEQIWIDEANQFGVSVTWKDNSIIGFVMRPLINYDAIKNETT
                     LKYAVPTTDCYEIFWGGDNELYNYHYLYPHQRYAIDLVIKENHKTYKNQGKVNTDYFC
                     FGKDIIAPANGTVEKVVNGVQDNSIGSTNESQFLGNYIVIKHAENEYSLIAHLHQYSI
                     IVNEGQNVKYGDIIGKVGNSGNSTEPHIHFQVMNDKNIEACTSLKIRFINNRELIKGD
                     VVCGLQAE"
     gene            complement(6723..9146)
                     /gene="hysA"
                     /locus_tag="O0293_001859"
     CDS             complement(6723..9146)
                     /gene="hysA"
                     /locus_tag="O0293_001859"
                     /EC_number="4.2.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase HysA"
                     /protein_id="HCY0280741.1"
                     /translation="MTYRIKKWQKLSTITLLMAGVITLNGGEFRSVDKHQIAVADTNV
                     QTPDYEKLRNTWLDVNYGYDKYDENNPDMKKKFDATEKEATNLLKEMKTESGRKYLWS
                     GAETLETNSSHMTRTYRNIEKIAEAMRNPKTTLNTDENKKKVKDALEWLHKNAYGKEP
                     DKKVKELSENFTKTTGKNTNLNWWDYEIGTPKSLTNTLILLNDQFSNEEKKKFTAPIK
                     TFAPDSDKILSSVGKAELAKGGNLVDISKVKLLECIIEEDKDMMKKSIDSFNKVFTYV
                     QDSATGKERNGFYKDGSYIDHQDVPYTGAYGVVLLEGISQMMPMIKETPFNDKTQNDT
                     TLKSWIDDGFMPLIYKGEMMDLSRGRAISRENETSHSASATVMKSLLRLSDAMDDSTK
                     AKYKKIVKSSVESDSSYKQNDYLNSYSDIDKMKSLMTDNSISKNGLTQQLKIYNDMDR
                     VTYHNKDLDFAFGLSMTSKNVARYESINGENLKGWHTGAGMSYLYNSDVKHYHDNFWV
                     TADMKRLSGTTTLDNEILKDTDDKKSSKTFVGGTKVDDQHASIGMDFENQDKTLTAKK
                     SYFILNDKIVFLGTGIKSTDSSKNPVTTIENRKANGYTLYTDDKQTTNSDNQENNSVF
                     LESTDTKKNIGYHFLNKPKITVKKESHTGKWKEINKSQKDTQKTDEYYEVTQKHSNSD
                     NKYGYVLYPGLSKDVFKTKKDEVTVVKQEDDFHVVKDNESVWAGVNYSNSTQTFDINN
                     TKVEVKAKGMFILKKKDDNTYECSFYNPESTNSASDIESKISMTGYSITNKNTSTSNE
                     SGVHFELTK"
     gene            complement(9143..9310)
                     /locus_tag="O0293_001860"
     CDS             complement(9143..9310)
                     /locus_tag="O0293_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280742.1"
                     /translation="MRKIWVIDCLYASTFFVDCLTVDSHLYTLTNFEKLNHYLIIIFI
                     AHAQHKKETCQ"
BASE COUNT         3175 a         1394 c         1571 g         3191 t
ORIGIN      
        1 gcaaaaccat atttgcattg tgtgttcaat aaagatattt taaatatgga taaagttgat
       61 atagaaaatt ttttcaaaag tatagaagtc aaatacagta aataagatca attattgaat
      121 ccaccgacca gcagattttt ttaaaaaatt aataccccgt taataccctt tgcttcaatt
      181 tgatgaaaat caatgaaatt caaaattgaa gaaatccttt aatatcaagg tgtacgacag
      241 tctatataat catgcgaaat tctaaaattt tctgaacgta aaaaaacaaa ttcttaaagc
      301 agctcgtcgt tcacctcaat tctcaaaacg ttaattgtcg gacgatatat atacaaaaca
      361 cctcgatgtt atgtcgaggt gtttttttgc gtttgtgtgg ggaatatgga atagagtata
      421 aatgaattaa tgtctcaggt atagaattaa ttcaactatg aattattgat ttacacgtat
      481 aatttgtaca tgcttacaca atcaaaagtg tacattatta aattgtcatt tagcttgcgg
      541 agaccgtacg atagtgttct aataatagat attcaaggga aagagctatc ggatgataac
      601 cagatgataa ctcaagaacc atgatttaag agtgttatca aaataaggag tgacttaatt
      661 gaaagattct atattttgga agaaagcttt tattcctgtt tattttattg ttgcgatgct
      721 ggtgtttcta ctttttaggt tttatattaa aacagataac ttttctatat atttaatgag
      781 tatcttctta atttgtttag gaactgcttc tatcatttat aactataaaa ccaatcgata
      841 aatataagta tgaaatgtat aagtcacagt tagatctaag tcttgctgtg gctttttata
      901 tctccataaa acgacaaatt caagcccgac ataaaacagc attttcagcc caccataaaa
      961 cgacaatttc agcccgccat tgactaaaca ccacatccca aaaatatcgt aacaatcctc
     1021 tacatcaatc aatccaacat ccctcataat cacaacgcac aaaatctatt catgcatttt
     1081 tggaatactt agtattacaa ataacgattt ttattcatct aacaaaggtt atataatgta
     1141 ctgaaggcaa tttttatgta ttacaaatct aatcgtacat gtaaaatttt gataaacatc
     1201 attaattttg cgtaactatc attagattac aaatcacaaa gtaattacat gtaatacaca
     1261 tctatacatc acatttgaag ggaaatgaat ataaatgact gataaaaagt acactgcagc
     1321 cgatatggtt attgatactt tgaaaaataa tggggtagaa tatgtttttg gtattccggg
     1381 tgcaaagatt gactatctat ttaatgcttt aattgatgat ggtcctgaac ttattgtcac
     1441 tcgtcatgaa caaaacgccg caatgatggc gcaaggtatt ggaagattaa caggcaaacc
     1501 gggtgtagta cttgttacaa gtggtcctgg tgtaagtaat ttaactactg gattattaac
     1561 cgcaacatct gaaggggatc ctgtattagc gttaggtggc caagtgaaac gtaatgattt
     1621 attacgatta acgcatcaaa gtattgataa tgctgcgcta ttaaaatatt catcaaaata
     1681 tagtgaagaa gtacaagatc ctgaatcatt atcagaagtt atgacaaatg caattcgaat
     1741 tgctacttca ggaaaaaatg gcgcaagttt tattagtatt ccgcaagacg ttatttcttc
     1801 accagttgaa tctaaagcta tatcactttg ccaaaaacca aatttaggag taccgagtga
     1861 acaagatatt aatgatgtca ttgaagcgat taaaaatgca tcatttcctg ttttattagc
     1921 tggtatgaga agttcaagtg cagaagaaac aaatgccatt cgcaaattag ttgagcgcac
     1981 gaatttacca gttgtagaaa cattccaagg tgcaggtgta attagtcgtg aattagaaaa
     2041 tcatttcttc ggtcgtgtgg gcttattccg caatcaagtt ggtgatgaat tattacgtaa
     2101 aagtgattta gttgttacaa tcggttatga tccaattgaa tacgaagcta gtaactggaa
     2161 taaagaatta gaaacacaaa ttatcaatat tgacgaagtt caagctgaaa ttactaatta
     2221 tatgcaaccg aaaaaagagt tgattggtaa tattgctaaa acgattgaaa tgatttctga
     2281 aaaagtggat gagccattta taaatcaaca acatttagac gaattagaac aattaagaac
     2341 acatattgat gaagaaactg gtattaaagc gacgcatgaa gaaggaattc tacatccagt
     2401 ggaaattatt gaatctatgc aaaaggtatt aactgatgat actactgtaa cagttgatgt
     2461 tggaagtcac tatatttgga tggcacgtaa tttcagaagt tacaatccaa gacatttatt
     2521 atttagcaat ggtatgcaaa cgcttggtgt agcattaccg tgggcaattt cagctgcact
     2581 tgtgcgccct aatacgcaag ttgtgtccgt tgctggcgat ggtggctttt tattttcatc
     2641 acaagattta gaaacggccg tacgtaaaaa tttaaatatc atccagctta tttggaatga
     2701 tggaaaatat aacatggttg aattccaaga agaaatgaaa tataaacgtt cgtcaggtgt
     2761 agacttcggt cctgtagatt ttgtaaaata tgcagaatca tttggcgcga aaggtttacg
     2821 agttactaat caagaagaat tagaagcggc aattaaagag ggctatgaaa cagatggtcc
     2881 agtattaatt gatatacctg taaattacaa agataatatc aaactttcaa caaatatgtt
     2941 acctgacgta tttaactaaa ataaagataa atgttaaaga ggagtgggag attttatgac
     3001 taatgtttta taccaacatg gtacattagg cacattaatg gcaggattat taaaaggaac
     3061 tgcatcaata aatgaattat tgcaacatgg tgacttaggt atcgctacac taacaggttc
     3121 aaacggtgag gtaatctttt tagatggaaa agcttaccat gcaaatgaac ataaagaatt
     3181 tgtagaatta aaaggtgacg agttaacacc atatgcaact gtaactaaat ttgtagcaga
     3241 tacaagctat gaaacgaaag ataaatcttc agaagcagtt tttgcagaaa ttaaggaaaa
     3301 gatgttgagt gaaaatttat tttcagcagt aaaaatttca ggcttattta aaaaaatgca
     3361 tgtacgtatg atgccggctc aagaaccacc ttatacacgt ttaatcgatt cagctagaag
     3421 acaacctgaa caaactgaaa cgtatgtcaa aggttcagtc gttggtttct ttacaccaga
     3481 attattccat ggtatcggat cagcaggatt tcatgtacac tttgcgaatg atgatcgtaa
     3541 ctttggtgga catgtcttag attttgaagt agaagatgtt aaagtagaaa tccaaaatat
     3601 agaaacattt gaacagcatt ttccaattca agataaagat ttcactaaag caaatattga
     3661 ctataaagat attgcagacg aaattagaga agctgaataa tgaatccaga aatataatga
     3721 cggtttatga aaattgactt cataatgcgc gatttagaaa tgatagtttg taaatatgat
     3781 taaccatgac tacaatagaa caaatatatt tataattacg tctaagtaat aaaataaatc
     3841 ccttcactat tagcagtagt gaggggattt attaggttcc agatatttga gatttgctgt
     3901 tatgtttaga ttataaattg tggtacacac tcatataaaa tttactattg tataggccaa
     3961 tctgttacta cgagaagcaa acaacaataa tttacaagtt caataactaa aaagacaaac
     4021 gccaattttt cagcgcttgc cctataaaac tattttcaaa ttattattta actacaattt
     4081 gtatctgttt gattgtactt ggatcaattg aatttttagc gtcgtcattt ttctttagat
     4141 tcactacttt tttattacca tcattcaaag taagcgtata agttgctgtt tgggcattat
     4201 taattttttc tgttgtaaca ccacgttgag aagctaattc attttttact ttactgtcaa
     4261 tttcttgata aagaacattt ttattttctg ggaagataaa gtaagttcga tgtaatgcag
     4321 taataccatc tactgaaatt gtgtaaggaa cagtgtgata accatccaca gtcatttctt
     4381 tatagccgtt attactatct gcagatgctt cgtgactcgg taatgcagct ccgaccgttg
     4441 ataataatcc caatgctaaa gtggcagtaa caaatgattt tagtttcata tctcaatctc
     4501 cttttcgggt tgtatgatat tacactattt gtataacacg tttcataaag tttaaaacat
     4561 actttttgca ttttttatat tttaattttt agtaatagtt tttaacgagg gatatgactc
     4621 gtgcattttg aaaagtggta agcaatgttt ttagtttata gggattgatt agttgtatat
     4681 aataatcatg aaaagcatat agtcgatctt atacttttaa ttgagactta tagtgtattt
     4741 aagttataga taaattcatt aaaatattat gggggaatga gtatgtcaaa gcgtctatta
     4801 ttgtttgatt ttgatgaaac atattttaaa cataatacaa atgaagaaga tttaagtcat
     4861 ttaagagaaa tggaaaaatt gttggaaaaa ctaactaata ataatgaagt gatcactgct
     4921 gtattgacag gaagtacatt tcaaagtgtc atggataaaa tggatcaagt taatatgacg
     4981 tttaaaccat tacatatctt ttctgatttg agttctaaaa tgttcacttg gaataatggc
     5041 gaatatgttg aatcagaaac atataaaaag aaagtcttga gcgaaccatt tttatttgaa
     5101 gatattgaag atatattacg tcatatttct gcgcaatata atgtcgaatt tattccacaa
     5161 agagcatttg aaggtaatga aacacattat aatttttatt ttcattcaac aggtaatcac
     5221 aacaatgata gtcgtatcct agaagcactt gtcagatacg caaatgacca aaactataca
     5281 gcgagattta gtcgaagcaa tccattagct ggtgatcctg aaaatgcgta tgatattgac
     5341 ttcacaccgt caaatgctgg aaaattatat gctactcaat ttctgatgag gaaatataat
     5401 ataccagtaa aatcaatatt aggttttgga gatagcggca atgacgaagc atatttaagc
     5461 tatcttgaac atgcatattt gatgtcgaat agtagagatg aagcattaaa gcagaaattt
     5521 agattaacaa aatatccata ttatcaaggg ataacactgc atgttaaaga atttgttgaa
     5581 ggtaagtatg attattgatt agttgagtga tatgtatact ttttaagtgc aaatattaat
     5641 atttaaggga tgattagaaa atatggtgaa tcgactacac tttagcttta aaaaattatt
     5701 tatatgttga tttatccaat taagtgtaga cgattcaggt agcgcaaaaa tgacgcagga
     5761 actggagaaa gaattgatac caaaattaat ataataggtt aagtatatat gaataaaaca
     5821 aactttgggg cttatttaaa taaaagtaag agcaggaaaa tatataagat ttttgatcaa
     5881 aatttgaagc gagagtttag ttattggcag tttaggaaac ttttcaaatc ttatacaagg
     5941 cagtctgatg tacatgaatt aaaagtgtgt actcaaaatg atggtactat tgagcaaata
     6001 tggatagatg aagcaaatca atttggagtg tcagtgactt ggaaagataa tagtattatc
     6061 ggatttgtta tgcgtccgtt aattaattat gatgcgatta agaatgaaac gactttaaaa
     6121 tacgcagtgc cgacaacaga ttgctatgag attttttggg gtggagataa tgaattatat
     6181 aattatcact atttataccc acatcaaagg tatgctattg atttagttat taaagaaaat
     6241 cataaaacat ataagaatca aggaaaagta aatactgatt atttttgttt tggaaaagat
     6301 atcattgcgc cagcaaatgg tacagttgaa aaagtagtga atggggttca agacaacagt
     6361 attggaagta cgaatgaatc acagttttta ggaaattata ttgtaattaa gcacgcagaa
     6421 aatgagtata gcttaatagc tcatttacac caatattcaa tcattgtgaa tgaggggcaa
     6481 aatgttaaat atggtgatat cattgggaag gttgggaatt ctggcaattc tactgaacct
     6541 catatacatt ttcaagtaat gaatgataag aatattgaag catgtacatc tttaaaaatt
     6601 cgatttataa ataatcgaga acttatcaaa ggggatgtgg tctgcggatt acaagctgaa
     6661 tgatggcgat acttataaaa tctcgacact ataaaaatgg tatagtgtcg agattttctt
     6721 gcttatttag ttaattcaaa gtgcacgccg gattcattag aagtcgacgt atttttgttt
     6781 gtaatagagt aaccggtcat tgaaatttta gattcaatat ctgaagcgga atttgtagat
     6841 tcaggattat agaagctaca ttcataagtg ttatcatctt tctttttaag tataaacata
     6901 cctttggctt taacttcgac tttagtgttg ttaatgtcaa aagtttgagt gctattacta
     6961 taattaacac cagcccaaac cgattcatta tctttcacaa cgtggaagtc atcttcttgc
     7021 ttaacgacag ttacttcatc tttttttgtc ttaaagacat ctttagataa gcctggatac
     7081 aacacatatc catatttatt gtcagaatta gaatgctttt gagttacttc ataatactca
     7141 tcagtttttt gtgtatcctt ttgactttta tttatttctt tccacttacc agtatgactt
     7201 tcttttttta cagttatttt cggtttgttt aaaaaatgat aaccgatgtt ctttttggta
     7261 tctgtggact ctaaaaagac tgaattgttt tcctgattat cagaattggt tgtttgttta
     7321 tcgtctgtat ataacgtata cccattcgct ttgcgatttt caatcgttgt aactggattc
     7381 tttgatgaat cagtactttt aatgccagtt cctaagaaga caattttatc gtttaatatg
     7441 aaatatgatt ttttggcagt taaagttttg tcctgatttt caaaatccat tccgatacta
     7501 gcatgttggt catcaacttt tgttccgcca acaaaagttt tactcgactt tttatcatcc
     7561 gtatctttta atatttcatt gtctaaagtt gttgtacctg ataaacgttt catatcggct
     7621 gtcacccaga agttatcatg atagtgtttg acatcgctgt tatataaata agacattcca
     7681 gcaccagtgt gccaaccttt taaattctct ccgttgatac tttcatagcg tgctacgttt
     7741 ttcgacgtca tacttaaacc aaatgcaaag tctaagtctt tgttatgata ggtgacacga
     7801 tccatgtcat tatatatttt aagttgttgt gttaatccgt ttttagaaat actgttatct
     7861 gtcattaaag acttcatttt atctatgtcc gaatatgaat ttaaataatc attttgttta
     7921 taacttgaat ctgactctac tgaagatttg acaatctttt tatacttagc ttttgttgaa
     7981 tcatccatgg catcacttaa tctcaacaat gatttcatta ctgttgcaga tgctgagtga
     8041 ctggtttcat tttcacgact gatagctcta cctcgtgata aatccatcat ttcaccttta
     8101 taaatgagtg gcataaatcc gtcgtcaatc catgacttta aggttgtatc attttgggtt
     8161 ttatcattaa aaggtgtttc ttttatcatc ggcatcattt gagaaatccc ctctaagagt
     8221 acaacgccat aagcaccagt gtatggaacg tcttgatgat caatgtaaga gccatcttta
     8281 taaaatccat tacgttcttt accagtggca gaatcttgaa cgtaagtgaa gactttatta
     8341 aatgaatcta tagacttttt catcatatct ttatcttctt cgataataca ttctaaaagt
     8401 ttcaccttag aaatgtctac tagatttccg cctttagcaa gttcagcttt tcctacagaa
     8461 gataatattt tgtcactatc tggggcgaaa gttttaatag gggcagtgaa ttttttcttt
     8521 tcttcatttg aaaattgatc attcagcaat ataagcgtat ttgttaatga tttaggtgtt
     8581 ccaatttcat aatcccacca atttaagttg gtattcttgc cagttgtttt agtaaaattc
     8641 tcacttaatt cttttacttt tttatctggt tcttttccat atgcattttt atgcagccac
     8701 tcaagggcat ctttcacttt cttcttattt tcgtcagtat ttaaagtggt tttaggattc
     8761 ctcatcgctt ctgcgatttt ctcaatatta cgataggtac gagtcatatg agaagaatta
     8821 gtttcaaggg tttccgctcc tgaccacaag tatttcctac cactttcagt tttcatttcc
     8881 ttgagtaaat tcgtcgcctc tttctctgta gcatcaaact tcttcttcat atctggatta
     8941 ttctcatcat acttatcata accatagtta acgtccagcc atgtgttcct caatttttca
     9001 taatctggcg tttgaacatt cgtatcagcc acagcgattt gatgtttatc aacacttctg
     9061 aattcaccac cattcaaagt aatcacacca gccattaata acgtaatggt ggataatttt
     9121 tgccatttct ttattctata tgtcattgac atgtctcctt tttgtgttgc gcgtgcgcaa
     9181 tgaatattat gattaaataa tgattcaatt tttcaaaatt cgttaacgta tacaaatgac
     9241 tgtctactgt caaacaatcc acaaagaatg ttgatgcata taaacaatcg atcacccaaa
     9301 ttttccgcat cacccaaaaa ttgcaactaa a
//
LOCUS       DAKTIO010000106         9166 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.106, whole genome shotgun sequence.
ACCESSION   DAKTIO010000106 DAKTIO010000000
VERSION     DAKTIO010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9166
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.106"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            125..1237
                     /gene="pdhA"
                     /locus_tag="O0293_001861"
     CDS             125..1237
                     /gene="pdhA"
                     /locus_tag="O0293_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HCY0280743.1"
                     /translation="MAPKLQAQFDAVKVLNDTQSKFEMVQILDENGNVVNEDLVPDLT
                     DEQLVELMERMVWTRILDQRSISLNRQGRLGFYAPTAGQEASQLASQYALEKEDYILP
                     GYRDVPQIIWHGLPLTEAFLFSRGHFKGNQFPEGVNALSPQIIIGAQYIQAAGVAFAL
                     KKRGKNAVAITYTGDGGSSQGDFYEGINFAAAYKAPAIFVIQNNNYAISTPRSKQTAA
                     ETLAQKAIAVGIPGIQVDGMDALAVYQATKEARDRAVAGEGPTLIETMTYRYGPHTMA
                     GDDPTRYRTSDEDAEWEKKDPLVRFRKFLENKGLWNEDKENEVIERAKADIKAAIKEA
                     DNTEKQTVTSLMEIMYEDMPQNLAEQYEIYKEKESK"
     gene            1241..2218
                     /locus_tag="O0293_001862"
     CDS             1241..2218
                     /locus_tag="O0293_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017637695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCY0280744.1"
                     /translation="MAQMTMVQAINDALKTELKNDQDVLIFGEDVGVNGGVFRVTEGL
                     QKEFGEDRVFDTPLAESGIGGLAMGLAVEGFRPVMEVQFLGFVFEVFDAIAGQIARTR
                     FRSGGTKTAPVTIRSPFGGGVHTPELHADNLEGILAQSPGLKVVIPSGPYDAKGLLIS
                     SIRSNDPVVYLEHMKLYRSFREEVPEEEYTIDIGKANVKKEGNDISIITYGAMVQESM
                     KAAEELEKDGYSVEVIDLRTVQPIDVDTIVASVEKTGRAVVVQEAQRQAGVGAAVVAE
                     LSERAILSLEAPIGRVAAADTIYPFTQAENVWLPNKNDIIEKAKETLEF"
     gene            2309..3601
                     /locus_tag="O0293_001863"
     CDS             2309..3601
                     /locus_tag="O0293_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HCY0280745.1"
                     /translation="MAFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKS
                     VVEIPSPVSGTVEEVMVEEGTVAVVGDVIVKIDAPDAEDMQFKGHDDDSSSKEEPAKE
                     EAPAEQAPVATQTEEVDENRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDA
                     YLNGGAPTASNESAASATSEEVAETPAAPAAVTLEGDFPETTEKIPAMRRAIAKAMVN
                     SKHTAPHVTLMDEIDVQALWDHRKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALN
                     TSFNEEAGEIVHKHYWNIGIAADTDRGLLVPVVKHADRKSIFQISDEINELAVKARDG
                     KLTADEMKGATCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPV
                     LALSLSFDHRQIDGATGQNAMNHIKRLLNNPELLLMEG"
     gene            3605..5011
                     /gene="lpdA"
                     /locus_tag="O0293_001864"
     CDS             3605..5011
                     /gene="lpdA"
                     /locus_tag="O0293_001864"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCY0280746.1"
                     /translation="MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLG
                     GVCLNVGCIPSKALLHASHRFVEAQHSENLGVIAESVSLNFQKVQEFKSSVVNKLTGG
                     VEGLLKGNKVNIVKGEAYFVDNNSLRVMDEKSAQTYNFKNGIIATGSRPIEIPNFKFG
                     KRVIDSTGALNLQEVPGKLVVVGGGYIGSELGTAFANFGSEVTILEGAKDILGGFEKQ
                     MTQPVKKGMKEKGVEIVTEAMAKSAEETDNGVKVTYEAKGEEKTIEADYVLVTVGRRP
                     NTDELGLEELGVKFADRGLLEVDKQSRTSISNIYAIGDIVPGLPLAHKASYEAKVAAE
                     AIDGQAAEVDYIGMPAVCFTEPELATVGYSEAQAKEEGLAIKASKFPYAANGRALSLD
                     DTNGFVKLITLKEDDTLIGAQVVGTGASDIISELGLAIEAGMNAEDIALTIHAHPTLG
                     EMTMEAAEKAIGYPIHTM"
     gene            5179..5454
                     /locus_tag="O0293_001865"
     CDS             5179..5454
                     /locus_tag="O0293_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HCY0280747.1"
                     /translation="MEYEYPIDLDWSNEEMISVINFFNHVEKYYESGVTAGDFMGAYK
                     RFKEIVPAKAEEKQIFNTFEKSSGYNSYKAVQDVKTHSEEQRVTAKK"
     gene            5598..6137
                     /locus_tag="O0293_001866"
     CDS             5598..6137
                     /locus_tag="O0293_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0280748.1"
                     /translation="MNIGNKIKNLRRIKNLTQEELAERTDLSKGYISQIESEHASPSM
                     ETFLNIIEVLGTTPSEFFKDSENEKVLYKKEEQVIYDEYDEGYILNWLVSKSNEYDME
                     PLILTLKPGASYKNFNPSESDTFIYCMSGQITLNLGKEIYQAQEEDVLYFKARDNHRL
                     SNESNNETRILIVATASYL"
     gene            6150..7244
                     /locus_tag="O0293_001867"
     CDS             6150..7244
                     /locus_tag="O0293_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0280749.1"
                     /translation="MEPLLSLKSVSKSYDDLNILDDIDIDIESGYFYTLLGPSGCGKT
                     TILKLIAGFEYPDSGEVIYQNKPIGNLPPNKRKVNTVFQDYALFPHLNVYDNIAFGLK
                     LKKLSKTEIDQKVTEALKLVKLSGYEKRNINEMSGGQKQRVAIARAIVNEPEILLLDE
                     SLSALDLKLRTEMQYELRELQSRLGITFIFVTHDQEEALALSDFLFVLKDGKIQQFGT
                     PTDIYDEPVNRFVADFIGESNIVEGRMVRDYVVNIYGQDFECVDMGIPENKKVEVVIR
                     PEDISLIKAEEGLFKATVDSMLFRGVHYEICCIDNKGYEWVIQTTKKAEVGSEVGLYF
                     DPEAIHIMVPGETEEEFDKRIESYEEVDNA"
     gene            7237..8034
                     /locus_tag="O0293_001868"
     CDS             7237..8034
                     /locus_tag="O0293_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0280750.1"
                     /translation="MRNTNKFLLIPYLLWMVIFIIVPVVLLIYFSFLDINGHFSFTNY
                     QQIFTTKYLKMFAYSILYAASITIITLTISYPAAYYITRSKFQNILLMIMIIPTWINL
                     LLKTYAFIGLLSHDGVINQFFHLFNLPSFNLLFTTGAFLVVASYIYIPFMILPIFNSM
                     KAIPNNLLQASSDLGASPFYTFRKVIMPLTKEGVMTGIQVTFIPSLSLFMITRLIAGN
                     KVINIGTAIEEQFLTIQNYGMGSTIAIFLIVFMAFILIITKSSNGRG"
     gene            8040..8849
                     /locus_tag="O0293_001869"
     CDS             8040..8849
                     /locus_tag="O0293_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0280751.1"
                     /translation="MKWYGKLYIGILLAILYIPIFFLMFYSFNSAGNMIHFEHFTLEH
                     YQSLFQNDHLMSVIFNTIAVALLAASISTVIGTFGAIAIYYLRNKKFKVTLLTLNNVL
                     MVSSDVVIGASFLIMFTTIGHFTGLGLGFWTVLISHIAFCIPIVVIIVLPQLYEMNNN
                     MLNAARDLGATEPQLLSNIIIPNILPSIIGGFFMALTYSLDDFTVSFFVTGNGFSVLS
                     VEVYAMARKGISMEINAISTLLFAVIVLGILGYYLIQYVINKKKLIKRGVK"
     gene            8849..>9166
                     /locus_tag="O0293_001870"
     CDS             8849..>9166
                     /locus_tag="O0293_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCY0280752.1"
                     /translation="MKRFLQLIIGALVVGMLCLTLSHWFKSKEQVHTNQKIYVYNWGE
                     YIDPELIKKFEKETGIQVVYETFDSNEAMEAKIRNGGTHYDVAFPSEYTVQKLKRDHL
                     LLPI"
BASE COUNT         3226 a         1344 c         1759 g         2837 t
ORIGIN      
        1 tctgtatctt tttttattaa aacagaactt tttcaaatgg tttaacagtc ccatttattt
       61 gtggtacaat tagtaaggat aaaatgaatt tctatacaat tatgggaaag gtatggtgaa
      121 ttgaatggct cctaagttac aagcccaatt cgatgcagta aaagttttaa atgatactca
      181 atcgaaattt gaaatggttc aaattttgga tgagaatggt aacgtcgtaa atgaagactt
      241 agtacctgat cttacggatg aacaattagt ggaattaatg gaaagaatgg tatggactcg
      301 tatccttgat caacgttcta tctcattaaa cagacaagga cgtttaggtt tctatgcacc
      361 aactgctggt caagaagcat cacaattagc gtcacaatac gctttagaaa aagaagatta
      421 cattttaccg ggatacagag atgttcctca aattatttgg catggtttac cattaactga
      481 agctttctta ttctcaagag gtcacttcaa aggaaatcaa ttccctgaag gcgttaatgc
      541 attaagccca caaattatta tcggtgcaca atacattcaa gctgctggtg ttgcatttgc
      601 acttaaaaaa cgtggtaaaa atgcagttgc aatcacttac actggtgacg gtggttcttc
      661 acaaggtgat ttctacgaag gtattaactt tgcagcagct tataaagcac ctgcaatttt
      721 cgttattcaa aacaataact atgcaatttc aacaccaaga agcaagcaaa ctgctgctga
      781 aacattagct caaaaagcaa ttgctgtagg tattcctggt atccaagttg atggtatgga
      841 tgcgttagct gtatatcaag caactaaaga agcacgtgac cgcgcagttg caggtgaagg
      901 tccaacatta attgaaacta tgacatatcg ttatggtcct catacaatgg ctggtgacga
      961 tccaactcgt tacagaactt cagacgaaga tgctgaatgg gagaaaaaag acccattagt
     1021 acgtttccgt aaattccttg aaaacaaagg tttatggaat gaagacaaag aaaatgaagt
     1081 tattgaacgt gcaaaagctg atattaaagc agcaattaaa gaggctgata acactgaaaa
     1141 acaaactgtt acttctctaa tggaaattat gtatgaagat atgcctcaaa acttagcaga
     1201 acaatatgaa atttacaaag agaaggagtc gaagtaagcc atggcacaaa tgacaatggt
     1261 tcaagcgatt aatgatgcgc ttaaaactga acttaaaaat gaccaagatg ttttaatttt
     1321 tggtgaagac gttggtgtta acggcggtgt tttccgtgtt actgaaggac tacaaaaaga
     1381 atttggtgaa gatagagtat tcgatacacc tttagctgaa tcaggtattg gtggtttagc
     1441 gatgggtctt gcagttgaag gattccgtcc ggttatggaa gtacaattct taggtttcgt
     1501 attcgaagta tttgatgcga ttgctggaca aattgcacgt actcgtttcc gttcaggcgg
     1561 tactaaaact gcacctgtaa caattcgtag cccatttggt ggtggcgtac acacaccaga
     1621 attacacgca gataacttag aaggtatttt agctcaatct ccaggtctaa aggttgttat
     1681 tccttcaggc ccatacgatg cgaaaggttt attaatttct tctattagaa gtaatgaccc
     1741 agtcgtatac ttagagcata tgaaattgta tcgttcattc cgtgaagaag tacctgaaga
     1801 agaatataca attgacattg gtaaggctaa tgtgaaaaaa gaaggtaatg acatttcaat
     1861 catcacatac ggtgcgatgg ttcaagaatc aatgaaagct gcagaagaac ttgaaaaaga
     1921 tggttattct gttgaagtaa ttgacttacg tactgttcaa ccaatcgatg ttgacacaat
     1981 tgtagcttca gttgaaaaaa ctggtcgtgc agttgtagtt caagaagcac aacgtcaagc
     2041 tggtgttggt gcagcagttg tagctgaatt aagtgaacgt gcaatccttt cattagaagc
     2101 acctattgga agagttgcag cagcagatac aatttatcca ttcactcaag ctgaaaatgt
     2161 ttggttacca aacaaaaatg acatcatcga aaaagcaaaa gaaactttag aattttaata
     2221 cattttaaaa gttaacgaag ttagcgtatt ttagtctcat tgattaaaat gaaatgttta
     2281 atttacgaaa tcttaggagg gcaaaaacgt ggcatttgaa tttagattac ccgatatcgg
     2341 ggaaggtatc cacgaaggtg aaattgtaaa atggtttgtt aaagctggag atactattga
     2401 agaagacgat gttttagctg aggtacaaaa cgataaatca gtagtagaaa tcccatcacc
     2461 agtatctggt actgtagaag aagttatggt agaagaaggt acagtagctg tagttggtga
     2521 cgttattgtt aaaatcgatg cacctgatgc agaagatatg caatttaaag gtcatgatga
     2581 tgattcatca tctaaagaag aacctgcgaa agaggaagcg ccagcagagc aagcacctgt
     2641 agctactcaa actgaagaag tagatgaaaa cagaactgtt aaagcaatgc cttcagtacg
     2701 taaatacgca cgtgaaaaag gtgttaacat taaagcagtt tctggatctg gtaaaaatgg
     2761 tcgtattaca aaagaagatg tagatgcata cttaaatggt ggtgcaccaa cagcttcaaa
     2821 tgaatcagct gcttcagcta caagtgaaga agttgctgaa actcctgcag cacctgcagc
     2881 agtaacatta gaaggcgact tcccagaaac aactgaaaaa atccctgcta tgcgtagagc
     2941 aattgcgaaa gcaatggtta actctaagca tactgcacct catgtaacat taatggatga
     3001 aattgatgtt caagcattat gggatcaccg taagaaattt aaagaaatcg cagctgaaca
     3061 aggtactaag ttaacattct taccttatgt tgttaaagca cttgtttctg cattgaaaaa
     3121 atacccagca cttaacactt cattcaatga agaagctggt gaaatcgttc ataaacatta
     3181 ctggaatatc ggtattgcag cagacactga tagaggatta ttagtacctg ttgttaaaca
     3241 tgctgatcgt aagtctattt tccaaatttc agatgaaatt aatgaattag ctgttaaagc
     3301 acgtgatggt aaattaacag ccgatgaaat gaaaggtgct acatgcacaa tcagtaatat
     3361 cggttcagct ggtggacaat ggttcactcc agttatcaat cacccagaag tagcaatctt
     3421 aggaattggc cgtattgctc aaaaacctat cgttaaagat ggagaaattg ttgcagcacc
     3481 agtattagca ttatcattaa gctttgacca cagacaaatt gatggtgcaa ctggccaaaa
     3541 tgcaatgaat cacattaaac gtttattaaa taatccagaa ttattattaa tggaggggta
     3601 aaacatggta gttggagatt tcccaattga aacagatact atagtaatcg gagcaggtcc
     3661 tggtggatac gttgcagcaa ttcgtgcagc tcaattagga caaaaagtaa caatcgttga
     3721 gaaaggtaat cttggtggtg tttgcttaaa cgtaggatgt attccttcaa aagcattact
     3781 acatgcttct caccgttttg ttgaagcaca acattctgaa aacttaggtg ttattgctga
     3841 aagtgtttct ttaaacttcc aaaaagttca agaattcaaa tcatcagttg ttaataaatt
     3901 aactggtggt gttgaaggct tacttaaagg taacaaagtt aacatcgtta aaggtgaagc
     3961 atatttcgta gataacaata gcttacgtgt tatggacgaa aagagcgcac aaacatacaa
     4021 ctttaaaaat ggaatcattg caacaggttc aagaccaatt gaaattccta atttcaaatt
     4081 cggtaaacgt gttatcgact caacaggtgc tttaaactta caagaagtac caggtaaatt
     4141 agttgtagtt ggtggaggat acattggatc agaattaggt acagcatttg ctaactttgg
     4201 ttcagaagta accatccttg aaggtgctaa agatatctta ggtggcttcg aaaaacaaat
     4261 gacacaacct gttaaaaaag gtatgaaaga aaaaggtgtt gaaatcgtta ctgaagctat
     4321 ggctaaatca gctgaagaaa cagataacgg agttaaagtt acttatgaag ctaaaggcga
     4381 agagaaaaca atcgaagctg attatgtatt agtaactgta ggtcgtcgtc caaacacaga
     4441 cgaattaggc ctagaagaat taggtgttaa attcgctgac cgtggattat tagaagttga
     4501 taaacaaagc cgtacgtcta tcagcaatat ctatgcaatt ggtgatatcg ttccaggttt
     4561 accacttgct cacaaagcta gctatgaagc taaagttgct gctgaagcaa ttgatggtca
     4621 agctgctgaa gttgattaca ttggtatgcc agcagtatgc tttactgaac cagaattagc
     4681 tacagttggt tattcagaag cgcaagctaa agaagaaggt ttagcaatta aagcttctaa
     4741 attcccatat gcagcaaatg gtcgtgcatt atcattagat gatactaacg gatttgttaa
     4801 acttattaca cttaaagaag atgatacttt aatcggtgct caagtagttg gtactggtgc
     4861 atcagatatt atctctgaat taggtttagc aattgaagct ggtatgaatg ctgaagatat
     4921 cgcattaaca atccatgcac atccaacatt aggtgagatg actatggaag cagcagaaaa
     4981 agctatcgga tacccaatcc atacaatgta ataactgatt atctataaag attcagtcat
     5041 taaaagctgt agcatatgct acggcttttt tgttttaggt aaagtaatgt aaggaaattg
     5101 atttgagata tcgttaacat gtgacatgca tgttatacta gcgatgctaa caaaagaatt
     5161 gaaatggagg gttcaacaat ggaatatgag tatccaattg atttagactg gagtaatgaa
     5221 gagatgattt cagtgataaa tttctttaat catgtagaga agtattatga atccggcgtg
     5281 acggcaggcg actttatggg tgcatataaa agatttaaag aaattgtgcc tgctaaagca
     5341 gaggaaaaac aaatttttaa tactttcgaa aaaagtagtg gctataatag ttacaaagca
     5401 gttcaagatg taaaaactca ctctgaagaa caaagagtaa cagctaaaaa ataattcgtt
     5461 cgaaattaac acaatttaat aggaattttt ctttaaaact attgctaata aagctatatt
     5521 ttgatacctt tatcaagtgt taaacaaaat gtttgataaa agtaaactta atatagcttt
     5581 tttaggtgga aaaataaatg aacataggta ataaaattaa aaatcttaga agaattaaaa
     5641 atttaacgca agaagaactt gctgaacgta cagacttatc gaaaggctac atttcacaaa
     5701 tagaaagtga acatgcctca ccaagtatgg aaactttctt aaatattata gaggtgttag
     5761 gaacgacgcc aagtgaattt tttaaagaca gtgaaaatga aaaagtatta tacaagaagg
     5821 aagaacaagt tatttatgat gagtatgatg aaggttatat attaaattgg ttagtttcaa
     5881 agtcaaatga atatgatatg gagccattaa tattaacttt aaagcctgga gcatcatata
     5941 aaaattttaa tccatcagag tctgatacgt ttatttattg tatgtcaggt cagataacac
     6001 ttaatttagg caaagagata tatcaagcac aagaagaaga cgttttgtat tttaaagcac
     6061 gagataatca tcgtttgtca aacgaatcaa acaatgaaac acgaatactt attgtagcga
     6121 cagcttcata tttatagggg ggatcttatt tggaaccgtt attatcatta aaatcagtta
     6181 gtaaaagcta tgatgatctt aatatcttag atgacataga tattgatatt gaatcaggat
     6241 acttttatac attattaggt ccttcaggtt gtggtaaaac aacaatttta aaattaattg
     6301 cagggtttga atatcctgac agtggtgaag tgatttatca aaacaaacca attggtaatt
     6361 taccaccaaa taaacgtaaa gtgaatacag tctttcaaga ttatgcatta tttccacact
     6421 taaacgtcta tgataatatc gcttttggtt tgaaattaaa aaaattatca aaaaccgaaa
     6481 ttgatcaaaa agtaactgag gcattaaaat tagtaaaact ttcaggttat gaaaaaagaa
     6541 atattaatga aatgagtggc ggacaaaagc aacgtgttgc aattgcacgt gctatcgtaa
     6601 atgaaccaga aatattattg ttagatgaat ctttatccgc attagatttg aaattgcgta
     6661 ctgaaatgca atatgaatta cgagaattgc aatctagatt aggtattaca tttatatttg
     6721 taacacatga tcaagaagaa gcgttagcat taagtgactt tctttttgta ttaaaagatg
     6781 ggaaaattca acaatttggc acaccaacag atatatatga cgaaccagtg aatcgatttg
     6841 tagctgattt tattggagaa tctaatattg ttgaagggcg catggttaga gattatgtcg
     6901 tgaatattta tgggcaagat ttcgaatgtg tcgatatggg tattcctgaa aataaaaaag
     6961 tagaagtcgt tattcgacca gaagatatat cattaatcaa agctgaagaa ggattattta
     7021 aagcaactgt tgattctatg ttatttagag gggtccacta tgaaatatgt tgtatagaca
     7081 ataaaggtta tgaatgggta atacaaacga ctaaaaaagc tgaagtaggc agtgaagttg
     7141 gtctttattt tgatcctgaa gccattcata ttatggttcc tggagaaaca gaagaagaat
     7201 ttgataaacg tattgaaagc tatgaggaag tagacaatgc gtaatactaa taaatttctc
     7261 ttaatcccgt atttactatg gatggttata tttattattg taccagttgt attactcatt
     7321 tatttttcat ttttagatat caatggacat tttagtttca cgaattatca acaaattttt
     7381 actacaaaat atttgaaaat gtttgcatat tcaattttat atgccgcttc aataacaatt
     7441 attaccttga ctatcagtta tccagctgcc tattatatta ctcgttcgaa atttcaaaat
     7501 atcttattaa tgataatgat tattccaaca tggataaatt tattgttaaa gacatatgct
     7561 tttataggtt tattaagtca tgatggcgtg attaatcaat ttttccactt atttaattta
     7621 ccatcattca atttgttatt tacaactggt gcgtttttag tagtggcaag ttacatttat
     7681 ataccattta tgattttacc tatatttaat agcatgaaag caattcctaa taatttattg
     7741 caggcctcaa gtgatttagg tgccagtcct ttctatactt ttagaaaagt aatcatgccg
     7801 ttaacaaaag aaggtgttat gactgggatt caagtaacct ttattccatc actttcatta
     7861 tttatgatta caagattaat tgcaggtaat aaagtcataa atataggtac ggcaatagag
     7921 gaacaatttt taacaattca aaattatggt atgggatcaa ctatagctat attcttaatt
     7981 gtatttatgg cattcatttt aatcattaca aaatcatcta atgggagagg gtgaatcata
     8041 tgaaatggta tggaaagctg tatatcggga tacttttagc gattttatac atcccaatat
     8101 tctttttaat gttctattca ttcaattcgg ctggtaatat gattcacttt gaacatttta
     8161 cattagagca ttatcaatca ttatttcaaa atgatcattt aatgtcggtc atttttaata
     8221 cgatagctgt agcactttta gcagcctcaa tttctacagt tattggtaca tttggtgcca
     8281 ttgctattta ttatttaaga aataaaaagt ttaaagtaac tttactaaca ttgaataatg
     8341 tcttgatggt atcatccgac gttgtcatag gtgcatcatt cttaattatg tttacaacga
     8401 ttggccattt tactggtctt ggtttaggat tttggacagt tctaatatct catattgcat
     8461 tttgcatacc tatagttgtg attatcgtct taccacaact gtatgaaatg aataataata
     8521 tgttaaatgc tgcaagagat ttaggagcga ctgaaccaca attattaagc aacattatta
     8581 ttcctaatat tttaccttct attataggag gattctttat ggctttaact tattcactag
     8641 acgatttcac agtaagtttc ttcgttactg gtaatggctt tagtgtgtta tcagttgaag
     8701 tttatgctat ggcgagaaaa ggaattagta tggaaattaa tgcgatttca acattattgt
     8761 ttgctgttat tgtattagga atactaggat attatttgat tcaatacgtg ataaataaga
     8821 aaaaactaat caagcgaggt gtaaaataat gaaacgattt ttacaactca ttataggtgc
     8881 attagttgtg ggtatgcttt gtcttacttt aagtcattgg tttaaatcta aagaacaagt
     8941 gcatacaaat caaaaaattt acgtatacaa ttggggcgaa tatattgatc cagagttaat
     9001 taagaaattt gaaaaagaaa ctggcattca agtcgtttat gaaactttcg attcaaatga
     9061 agcgatggaa gccaaaattc gcaatggcgg tacacattat gatgttgctt ttcctagtga
     9121 atatacagtt caaaaattga aaagagatca tttattgtta ccaata
//
LOCUS       DAKTIO010000107         9056 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.107, whole genome shotgun sequence.
ACCESSION   DAKTIO010000107 DAKTIO010000000
VERSION     DAKTIO010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9056
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.107"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..78
                     /locus_tag="O0293_001871"
     CDS             <1..78
                     /locus_tag="O0293_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenyl pyrophosphate synthase subunit A"
                     /protein_id="HCY0280753.1"
                     /translation="KKYNQSEVKHYLHDIQKSYLKSRGN"
     gene            81..806
                     /locus_tag="O0293_001872"
     CDS             81..806
                     /locus_tag="O0293_001872"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HCY0280754.1"
                     /translation="MADNKANKEQVHRVFQNISKKYDRLNNIISFEQHKVWRKRVMKD
                     MGVRKGTKALDVCCGTGDWTIALSKAVGPTGEVTGIDFSENMLEVGKEKTASMENVKL
                     VHGDAMELPFEDNSFDYVTIGFGLRNVPDYLVALKEMNRVLKPGGMVVCLETSQPTLP
                     VFKQMYALYFKFVMPIFGKLFAKSKEEYEWLQQSTFNFPGKEELKRMFEEAGFINVRV
                     RSFTGGVAAMHLGYKEKDNTKGD"
     gene            808..1767
                     /locus_tag="O0293_001873"
     CDS             808..1767
                     /locus_tag="O0293_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCY0280755.1"
                     /translation="MAKLNMNNEIKKVEQRLEKAIKSKDSVLEQASLHLLSSGGKRVR
                     PAFVILSSQFGKDEQTSEQTYQVAVALELIHMATLVHDDVIDKSDKRRGKLTISKKWD
                     QTTAILTGNFLLALGLEHLMAVKDNRVHQLISESIVDVCRGELFQFQDQFNSQQTIIN
                     YLRRINRKTALLIQISTEVGAITSQSDKETVRKLKMIGHYIGMSFQIIDDVLDFTSTE
                     KKLGKPVGSDLLNGHITLPILLEMRKNPDFKLKIEQLRRDSERKEFEECIQIIRKSDS
                     IDEAKAVSSKYLSKALNLISELPDGHPKSLLLSLTKKMGSRNT"
     gene            1859..2308
                     /gene="ndk"
                     /locus_tag="O0293_001874"
     CDS             1859..2308
                     /gene="ndk"
                     /locus_tag="O0293_001874"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCY0280756.1"
                     /translation="MERTFLMIKPDAVQRNLIGEVISRIERKGLKLVGGKLMQVPMEL
                     AETHYGEHQGKPFYNDLISFITSAPVFAMVVEGEDAVNVSRHIIGSTNPSEASPGSIR
                     GDLGLTVGRNIIHGSDSLESAEREINLWFNENEITSYASPRDAWLYE"
     gene            2517..2717
                     /locus_tag="O0293_001875"
     CDS             2517..2717
                     /locus_tag="O0293_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280757.1"
                     /translation="MKNIISIILGILMFLKLMELLYGAIFLDKPLNPITKIIFILTLI
                     YIFYVLVKELIIFLKSKYNKSA"
     gene            3103..4269
                     /gene="aroC"
                     /locus_tag="O0293_001876"
     CDS             3103..4269
                     /gene="aroC"
                     /locus_tag="O0293_001876"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCY0280758.1"
                     /translation="MRYLTSGESHGPQLTVIVEGVPANIEIKVEDINKEMFKRQGGYG
                     RGRRMQIEKDTVEIVSGVRNGYTLGSPITMVVTNDDFTHWRKIMGAAPISEEERENMK
                     RTITKPRPGHADLVGGMKYNHRDLRNVLERSSARETAARVAVGALCKVLLQQLDIDIY
                     SRVVEIGGIKDKDFYDSETFKANLDRNDVRVIDDSIAQAMRDKIDEAKNEGDSIGGVV
                     QVVVENMPVGVGSYVHYDRKLDGKIAQGVVSINAFKGVSFGEGFKAAEKPGSEIQDEI
                     LYNSEIGYYRGSNHLGGLEGGMSNGMPIIVNGVMKPIPTLYKPLNSVDINTKEDFKAT
                     IERSDSCAVPAASIVCEHVVAFEIAKALLEEFQSNHIEQLKQQIIERRQLNIEF"
     gene            4295..5359
                     /locus_tag="O0293_001877"
     CDS             4295..5359
                     /locus_tag="O0293_001877"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCY0280759.1"
                     /translation="MKLQTTYPSNNYPIYVEHGAIDHISTYIDQFDQSFILIDEHVNQ
                     YFADKFDDILSYENVHKVIIPAGEKTKTFEQYQETLEYILSHHVTRNTAIIAVGGGAT
                     GDFAGFIAATLLRGVHFIQVPTTILAHDSSVGGKVGINSKQGKNLIGAFYRPTAVIYD
                     LDFLKTLPFEQILSGYAEVYKHALLNGESATQDIEQHFKDREILQSLNGMDKYIAKGI
                     ETKLDIVIADEKEQGVRKFLNLGHTFGHAVEYYHKIPHGHAVMVGIIYQFIVANALFD
                     SKHDINHYIQYLIQLGYPLDMITDLDFETLYQYMLSDKKNDKQGVQMVLIRQFGDIVV
                     QHVDQLTLQHACEQLKTYFK"
     gene            5369..6667
                     /gene="aroA"
                     /locus_tag="O0293_001878"
     CDS             5369..6667
                     /gene="aroA"
                     /locus_tag="O0293_001878"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCY0280760.1"
                     /translation="MVNEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGVSTIYK
                     PLLGEDCRRTMDIFRLLGVEIKEDDEKLVVTSPGYQSFNTPHQVLYTGNSGTTTRLLA
                     GLLSGLGIESVLSGDVSIGKRPMDRVLRPLKLMDANIEGIEDNYTPLIIKPSVIKGIN
                     YQMEVASAQVKSAILFASLFSKEPTIIKELDVSRNHTETMFKHFNIPIEAEGLSINTT
                     PEAIRYIKPADFHVPGDISSAAFFIVAALITPGSDVTIHNVGINPTRSGIIDIVEKMG
                     GNIQLFNQTTGAEPTASIRIQYTPMLQPITIEGELVPKAIDELPVIALLCTQAVGTST
                     IKDAEELKVKETNRIDTTADMLNLLGFELQPTNDGLIIHPSEFKTNATVDSLTDHRIG
                     MMLAVASLLSSEPVKIKQFDAVNVSFPGFLPKLKLLENEG"
     gene            6674..7918
                     /locus_tag="O0293_001879"
     CDS             6674..7918
                     /locus_tag="O0293_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280761.1"
                     /translation="MEDIYKLIDDINLQKLENLDSRVNEAITTDNDDALFILGETLYN
                     FGLMPQGLEVFRVLYHKYPDESELLIYFIEGLMSENQTDEALEYLSYVEPSPEKLMLE
                     ADLYQQINMMEVAIDKLQEALELEPNDPIIHFALAEMLYYDGQYLRATSEYETVLETG
                     EYQVNGVNLFSRMADCSLQSGNYSDAIRLYDEINEDEMTSEDYLKKAISYDKNDITQE
                     AIKIMTTLLSKDPDYIQGYLYLQSLYENEKNYPDAIETGKEGLRLSQFYKELMYTTGC
                     LEIEHGDANEGVLLLKQALEVDNAYQEPLLILSDLYRNEEDYEAIIELLTYVDEEDLD
                     PTFMWHLAFAYGQEERDKEAQHFFELAYPTMKTNIDFMSDYYFYLIEIGQKEQAITIL
                     NQLLELEPSNENWHDELSRLQS"
     gene            7932..8507
                     /locus_tag="O0293_001880"
     CDS             7932..8507
                     /locus_tag="O0293_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HCY0280762.1"
                     /translation="MSETFNQIKESFIEYLLFQYRFKSRIAVWVLNYIKVNEAKLANI
                     HFVDTKINHHETLEIAEVGSHASAIQFTKRNIKLMNTNEIFDYIANHNCAFDIQIHFA
                     NVSKREQRLDDLIVAQLTESPSYQTYLHDLNSMAIDRHKHALLIDYLLHNIDLSLQMN
                     EKQRFYQLTQILNTLKLVNKHNQFEDLADDN"
     gene            8497..>9056
                     /locus_tag="O0293_001881"
     CDS             8497..>9056
                     /locus_tag="O0293_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1405 domain-containing protein"
                     /protein_id="HCY0280763.1"
                     /translation="MTIKAFWQYTLYQRSWLMMLLICNILGMIYGYIWYGEQLSHTPW
                     HFKIFVPDSPTAILYLVISISLILIQKQNSIIDALAFVTLFKYGIWAVIMNILFIIEQ
                     GDITVNGLVLMFSHSIMAVQAIYFYPRFKRSMIGISVAMIWVFLNDYIDYFHLQFPYY
                     DFITTHVWQIGVLSCCLSVFGLLLYI"
BASE COUNT         3244 a         1219 c         1706 g         2887 t
ORIGIN      
        1 aagaaatata atcaaagtga agtaaagcat tacttacacg atatccaaaa aagttattta
       61 aaaagtagag gtaattaagc atggctgaca ataaagcaaa taaagagcaa gtacatcgcg
      121 tttttcaaaa tatttcaaaa aaatatgata gattaaataa tattattagt tttgagcagc
      181 ataaagtatg gagaaaacgc gtcatgaaag acatgggtgt tagaaaaggg acgaaagcat
      241 tagatgtttg ttgtggtact ggtgattgga caatcgcatt aagcaaagct gtaggaccaa
      301 caggtgaagt tactggtatt gactttagtg agaatatgtt agaagtagga aaagaaaaaa
      361 ctgcttcaat ggaaaatgtt aaacttgttc atggtgatgc aatggaattg ccgtttgaag
      421 acaattcttt tgattatgta acaattgggt ttggattaag aaatgtgcca gactatttag
      481 tcgcgttaaa agaaatgaat agagtactta aaccaggtgg tatggtggta tgtcttgaaa
      541 cgagccaacc aactttgcca gtctttaaac aaatgtatgc actatacttt aaatttgtta
      601 tgcctatttt tggaaaatta tttgcaaaat caaaagaaga atatgaatgg ttacagcaat
      661 ctacgtttaa ttttcctgga aaagaagagt tgaagcgcat gtttgaagaa gcaggtttca
      721 taaatgtaag agtacgtagt tttacagggg gcgttgctgc aatgcacctt ggctataaag
      781 aaaaagataa taccaaaggt gattaacgtg gcaaagttaa acatgaacaa tgaaattaag
      841 aaagtggaac aacgacttga aaaggcaata aaaagtaaag attctgtatt agaacaggca
      901 tcattacact tattgtcttc tggtggtaaa agagtacgcc cagcatttgt tattctgagt
      961 agccaatttg gcaaagatga acaaacgtct gaacaaacgt atcaagttgc agtcgcatta
     1021 gagttaattc atatggcaac acttgttcat gatgacgtta ttgataaaag cgacaagcgt
     1081 cgaggcaagt taaccatatc aaagaaatgg gatcagacaa ctgctatttt aactgggaat
     1141 tttttattgg cattaggact tgaacactta atggccgtta aagataatcg tgtacatcaa
     1201 ttgatatctg aatctatcgt tgatgtttgt agaggggaac ttttccaatt tcaagaccaa
     1261 tttaacagtc aacagacaat tattaattat ttacgacgta tcaatcgcaa aacagcactg
     1321 ttaattcaaa tatcaactga agttggtgca attacttctc aatctgataa agagactgta
     1381 cgaaaattga aaatgattgg tcattatata ggtatgagct tccaaatcat tgatgatgta
     1441 ttagacttca caagtaccga aaagaaatta ggtaagccgg tcggaagtga tttgcttaat
     1501 ggtcatatta cgttaccgat tttattagaa atgcgtaaaa atccagactt caaattgaaa
     1561 atcgaacagt tacgtcgtga tagtgaacgc aaagaatttg aagaatgtat ccaaatcatt
     1621 agaaaatctg acagcatcga tgaggctaag gcagtaagtt cgaagtattt aagtaaagct
     1681 ttgaatttga tttctgagtt accagatgga catccgaaat cactactttt aagtttgacg
     1741 aaaaaaatgg gttcaagaaa cacgtagtat ttatgaaaag tattgaaagc gctttaccaa
     1801 cctgttaata tataatagta atatacataa acctattaaa cacaggggga tacataaagt
     1861 ggaacgtaca tttttaatga ttaaaccaga tgcagtacaa agaaatctaa ttggtgaagt
     1921 aatttcaaga attgaaagaa aaggactaaa acttgtcggt ggtaaattaa tgcaagtacc
     1981 aatggaactt gctgaaacac attatggtga acaccaaggt aaaccatttt ataatgattt
     2041 aatttcattt attacatcag caccagtgtt cgcaatggta gttgaaggtg aagatgcagt
     2101 taatgtatct agacatatta ttggcagcac caatccttca gaagcttcac caggatcaat
     2161 tagaggtgat ttaggtttaa ctgttggtag aaatatcatt cacggttcag attcattaga
     2221 gtctgctgaa cgtgaaatta atctatggtt taatgaaaat gaaattacta gctatgcttc
     2281 accacgtgat gcatggttat atgaataaaa tataaactgt aaacctttac gatttattta
     2341 taaaggtaga aagggttttg ttatgtggtt agtcattatg attatacata acaaggcccg
     2401 ttttttatgt tgtagtaaat tacttgaaaa attttatagt tttttggtaa cacgtattaa
     2461 aaagagagga atattcttta tcaaatgaaa ctaaacagag agaaggggtt gttaaaatga
     2521 agaatattat ttcgattatt ttggggattt taatgttctt aaaattaatg gaattactat
     2581 atggtgctat atttttagat aaaccactta atcctataac aaaaattatt tttatactga
     2641 ctctcattta tattttttat gtattagtaa aagaattgat tatatttttg aagtcaaagt
     2701 ataacaaaag cgcttaacat atgtatattt taatatcata atttttttaa acggactgat
     2761 taactttatt aataattaac agttcgttct tttgtattaa gaaatgtagt cagtatatta
     2821 tttgctaaag ttgcgatacg attatattaa aacggctaat catttttaat taatgattat
     2881 atgatgcaac tgtttagaaa ttcatgatac ttttctacag acgaatatat tataattaat
     2941 tttagttcgt ttaatattaa gataattctg acatttaaaa tgagatgtca tccattttct
     3001 taattgagct tgaaaacaaa catttatgaa tgcacaatga atatgataag attaacaaca
     3061 tattataatg ttatcgtgga agtatgaaag gagcgagtgt gtatgagata cctaacatca
     3121 ggagaatcac atggacctca attaacagtt attgttgaag gtgtacctgc aaatatagaa
     3181 attaaggttg aggatattaa taaagaaatg tttaagcgtc aaggcggtta cggacgtgga
     3241 cgtcgtatgc aaattgagaa agatacagta gaaatagtat caggcgttag aaatggttat
     3301 acattaggta gtccaattac tatggttgta accaatgatg actttacgca ttggagaaaa
     3361 attatgggag cagctccaat aagtgaagaa gaacgtgaaa atatgaaacg tactattaca
     3421 aaaccaagac ctggtcatgc agatttggtt ggaggtatga aatataatca tcgtgattta
     3481 cgaaatgtgc tagagcgatc atctgctaga gaaacagcag ctcgagttgc agtcggtgcc
     3541 ttatgtaaag tgttattaca acagttagat atcgatatat acagtcgtgt tgttgaaata
     3601 ggtggaatta aagataaaga tttttatgat tcagaaacat ttaaagcaaa tcttgatcgt
     3661 aatgatgttc gtgtaattga tgacagtatc gcacaagcaa tgcgagataa aattgacgaa
     3721 gctaaaaatg aaggagattc aattggcggt gtcgttcaag ttgtagttga aaatatgcct
     3781 gttggtgtag gtagttatgt gcattatgat cgtaagttag atggtaagat tgcacaaggt
     3841 gttgtcagca taaatgcttt taaaggtgta agctttggtg aaggatttaa agcagctgaa
     3901 aagccaggta gtgagattca agatgaaatt ctatataata gtgaaattgg ttattatcgt
     3961 ggatctaatc acttaggtgg tttagaaggc ggtatgtcaa atggaatgcc aattatcgtt
     4021 aatggtgtaa tgaaaccaat tccaacgtta tataaaccat taaattcagt agacattaat
     4081 actaaagaag actttaaagc aacaattgaa cgttctgata gttgtgctgt tcctgcagca
     4141 agtatcgtct gcgaacatgt cgtagcattt gaaatagcaa aagcattatt ggaagaattc
     4201 caatcaaatc atattgagca acttaaacaa caaattattg agcgcagaca attaaatatt
     4261 gagttttaac aacaagaaca attgaggtgt aatcatgaaa ttacaaacaa catacccttc
     4321 aaataattat ccaatatatg ttgaacacgg tgcaattgac catattagca cgtatattga
     4381 tcagtttgat caaagtttta tattaattga cgagcatgta aatcaatatt ttgctgataa
     4441 atttgatgat attttatcat atgaaaatgt acataaagtt attattccag ctggtgaaaa
     4501 gacgaaaaca tttgagcaat atcaagaaac attagagtat attttatccc atcatgtaac
     4561 tcgtaataca gcaattattg ctgttggtgg tggtgcaact ggtgattttg ctgggtttat
     4621 tgcggcgaca cttttacgag gcgttcactt tatacaagtg ccaacgacta tactagcgca
     4681 tgattctagt gttggcggta aagtgggtat taactcaaag caaggtaaaa accttatcgg
     4741 tgcattttat cgtccaactg ctgtgattta tgatttagac tttttaaaga cgttaccatt
     4801 tgagcaaata ttaagtggct atgcagaagt ttataagcat gcgttattga atggtgaatc
     4861 agcgacgcaa gatatcgaac agcactttaa agatagagag atattacagt cattaaatgg
     4921 tatggataaa tatattgcta aaggtattga aacgaagctg gatattgtta ttgcagatga
     4981 aaaagaacaa ggtgtacgta aatttttaaa tttaggtcat acatttggtc atgctgttga
     5041 atactatcat aaaatacctc atggtcatgc agtgatggtt ggcattatct atcaatttat
     5101 agttgcgaat gctttgtttg attctaagca tgatattaat cattatattc aatatttaat
     5161 acaactcggc tatcctttag acatgataac tgacttggat tttgaaacgt tataccaata
     5221 tatgctaagt gataaaaaga atgataagca aggtgtacaa atggtcttga ttagacaatt
     5281 tggagatatc gttgtacaac atgttgatca actaacatta caacatgcat gtgaacaatt
     5341 aaaaacatat tttaagtagg tgaatgaaat ggtaaatgaa caaatcattg atatttcagg
     5401 tccgttaaag ggcgaaatag aagtgccggg cgataagtca atgacacacc gtgcaatcat
     5461 gttggcgtcg ctagctgaag gtgtatctac tatatataag ccactacttg gcgaagattg
     5521 tcgtcgtacg atggacattt tccgactgtt aggtgtagaa atcaaagaag atgatgaaaa
     5581 attagttgtg acttccccag gatatcaatc ttttaacacg ccacatcaag tattgtatac
     5641 aggtaattct ggtacgacaa cacgattatt ggcaggtttg ttaagtggtt taggtattga
     5701 aagtgttttg tctggcgatg tttcaattgg taaaaggcca atggatcgtg tcttgagacc
     5761 attgaaactt atggatgcga atattgaagg tattgaagat aattatacac cattaattat
     5821 taagccatct gtcataaaag gtataaatta tcaaatggaa gttgcaagtg cacaagtaaa
     5881 aagtgccatt ttatttgcaa gtttgttttc taaggaaccg accatcatta aagaattaga
     5941 tgtaagtcga aatcatactg agacgatgtt caaacatttt aatattccaa ttgaagcaga
     6001 agggttatca attaatacaa cccctgaagc aattcgatac attaaacctg cagattttca
     6061 tgttcctggc gatatttcat ctgcagcgtt ctttattgtt gcagcactta tcacaccagg
     6121 aagtgatgta acaattcata atgttggaat caatccaaca cgttcaggta ttattgatat
     6181 tgttgaaaaa atgggcggta atatccaact tttcaatcaa acaactggtg ctgaacctac
     6241 tgcttctatt cgtattcaat acacaccaat gcttcaacca ataacaatcg aaggagaatt
     6301 agttccaaaa gcaattgatg aactgcctgt aatagcatta ctttgtacac aagcagttgg
     6361 cacgagtaca attaaagatg ccgaggaatt aaaagtaaaa gaaacaaata gaattgatac
     6421 aacggctgat atgttaaact tgttagggtt tgaattacaa ccaactaatg atggattgat
     6481 tattcatccg tcagaattta aaacaaatgc aacagttgat agtttaactg atcatcgaat
     6541 aggaatgatg cttgcagttg cttctctact ttcaagcgag cctgtcaaaa tcaaacaatt
     6601 tgatgctgta aatgtatcat ttccaggatt tttaccaaaa ctaaagcttt tagaaaatga
     6661 gggataatat aaaatggaag atatctataa attaatagac gatatcaatc tacaaaaact
     6721 agaaaattta gactctcgtg ttaatgaagc aataactact gacaacgatg acgcattatt
     6781 tattctagga gagacacttt acaattttgg attaatgcca caaggtttgg aagtattccg
     6841 cgtgttatat cacaaatatc cagacgaaag tgaattgctg atttatttta ttgaaggttt
     6901 aatgtctgaa aatcaaactg acgaagcgtt agaatattta agttatgttg aaccatcacc
     6961 tgaaaagttg atgttagaag cagatttata tcaacaaatt aatatgatgg aagttgctat
     7021 tgataaatta caagaagcac ttgaactaga gccaaatgat ccaataatcc attttgcatt
     7081 ggctgaaatg ttatattatg atggtcaata tttacgtgct acctctgaat acgaaaccgt
     7141 tttagaaact ggtgaatatc aagttaatgg tgtaaactta ttctctcgta tggcagattg
     7201 tagtttacaa agtggtaact atagtgatgc gattcgctta tacgatgaaa ttaatgaaga
     7261 tgaaatgact tcagaagatt atctcaaaaa agccatttct tacgataaaa atgacatcac
     7321 tcaagaagca attaaaataa tgactacatt actttctaaa gatcctgatt atattcaagg
     7381 ctacttgtat ttacaatcat tatatgaaaa tgaaaaaaat tatccagatg ccattgaaac
     7441 gggtaaagaa ggattacgac taagtcaatt ttataaagag ttaatgtata caactggatg
     7501 tttggaaata gaacacggcg atgctaatga aggtgtatta ttgttaaaac aagcattaga
     7561 ggttgataat gcttaccaag aacctttatt gattttaagc gatttatatc gtaatgaaga
     7621 agattatgaa gcaatcattg aattattaac atatgtagat gaagaagatt tggatcctac
     7681 attcatgtgg catttagcgt tcgcctatgg tcaagaagag cgagataaag aagctcaaca
     7741 tttctttgaa ttagcatatc caacgatgaa aacaaatatt gattttatga gtgattatta
     7801 tttctattta attgaaattg gtcaaaagga acaagcaatt actattttaa atcaattgtt
     7861 ggaactagag ccaagtaacg aaaattggca tgacgaatta tcaagattgc aatcttaaaa
     7921 tggggtattt gatgtcagag acattcaatc aaattaaaga aagttttatt gaatatttac
     7981 tttttcaata tcgatttaag tcgcgtattg cagtttgggt gcttaactat ataaaagtga
     8041 atgaggcgaa attggcaaac attcattttg tagatacaaa aattaatcat catgaaacat
     8101 tagaaattgc agaagtggga agtcacgctt ctgcaattca atttactaag cgtaatataa
     8161 agttaatgaa tacaaatgaa atatttgatt atatcgctaa tcataactgt gcttttgata
     8221 ttcaaattca cttcgcaaac gtgtcaaagc gtgagcaaag gctagatgat ttaatagttg
     8281 ctcaactaac ggaatcgccc agttatcaaa cttatcttca tgacttaaat agtatggcta
     8341 tagatcggca taaacatgcg ttattaatag attacttgtt acacaatatt gatttaagtt
     8401 tacagatgaa tgaaaagcaa cgtttttatc aactaacgca aattttaaat acactcaagc
     8461 tagtaaataa acataatcaa tttgaggact tagcagatga caattaaagc attttggcaa
     8521 tatacacttt atcaacgttc ttggttaatg atgctactca tttgtaatat attagggatg
     8581 atttatggtt atatatggta tggtgaacaa ttaagtcata cgccctggca ttttaagatt
     8641 tttgttccag atagcccaac tgcaatatta tacttagtta tttctatctc attgatatta
     8701 attcaaaaac aaaatagtat tatcgatgca ctggcatttg taacattatt caaatatggg
     8761 atttgggcag ttataatgaa tatattattt attattgaac aaggtgatat tacagtaaat
     8821 ggtttggtat taatgttttc ccacagtata atggctgttc aggcaattta cttctatcct
     8881 aggtttaaac gttctatgat tggaatcagt gtagcgatga tttgggtgtt tttgaatgat
     8941 tatattgact attttcacct tcaatttcca tattatgatt ttataactac acatgtgtgg
     9001 caaattggtg tattatcatg ttgtctaagt gtttttggac ttttgcttta tattga
//
LOCUS       DAKTIO010000108         9053 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.108, whole genome shotgun sequence.
ACCESSION   DAKTIO010000108 DAKTIO010000000
VERSION     DAKTIO010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9053
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.108"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..272
                     /locus_tag="O0293_001882"
     CDS             <1..272
                     /locus_tag="O0293_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3885 domain-containing protein"
                     /protein_id="HCY0280764.1"
                     /translation="EFSYKCSIKDIKYKKLLKAISNQDFKGLVPNINQNDNYSEIYII
                     NNTKNLIYHLYDDRGLWLAFNNNEDYARYSEKYNDLISEFDNEDI"
     gene            complement(365..526)
                     /locus_tag="O0293_001883"
     CDS             complement(365..526)
                     /locus_tag="O0293_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280765.1"
                     /translation="MLLILELRFWNLEFGFRNSTFGIQNYNEWKILIIACLMTWFCSD
                     FYLMNCFFV"
     gene            complement(563..1303)
                     /locus_tag="O0293_001884"
     CDS             complement(563..1303)
                     /locus_tag="O0293_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCY0280766.1"
                     /translation="MFNLKHDLETLKHIIDSSNRITFFTGAGVSVASGVPDFRSMGGL
                     FDEISKDGLSPEYLLSRDYLEDDPEGFINFCHKRLLFVDTMPNIVHDWIAKLERNQQS
                     LGVITQNIDGLHSDAGSQHVDELHGTLNRFYCNVCHKSYTKSDVIDRTLKHCDNCGGA
                     IRPDIVLYGEMLDQPTIIRALNKIEHADTLVVLGSSLVVQPAAGLISHFKGDNLIIIN
                     KDRTPYDSDATLVIHDDMVSVVKSLMTE"
     gene            1509..2300
                     /locus_tag="O0293_001885"
     CDS             1509..2300
                     /locus_tag="O0293_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCY0280767.1"
                     /translation="MTHRLNKGGNFTLNKHERLDEIAKLVNKKGTIRTNEIVEGLNVS
                     DMTVRRDLIELENKGILTKIHGGARSNSTFQYKEISHKEKHTRQIAEKRFIAKKAASL
                     IEDGDTLFFGPGTTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMR
                     HITEAFVGEMANAMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYL
                     LIDHTKVGKEDFTSFCQLNELTAVVMDYEDEEKVETIKTYIEVVD"
     gene            2668..3096
                     /gene="lacA"
                     /locus_tag="O0293_001886"
     CDS             2668..3096
                     /gene="lacA"
                     /locus_tag="O0293_001886"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HCY0280768.1"
                     /translation="MAIIIGSDEAGKRLKEVIKSYLLDNKYDVVDVTEGQEVDFVDAT
                     LAVAKDVQSQEGNLGIVIDAFGAGSFMVATKIKGMIAAEVSDERSGYMTRGHNNSRMI
                     TMGSEIVGDTLAKNVVKGFVEGKYDGGRHQIRVDMLNKMC"
     gene            3112..3627
                     /gene="lacB"
                     /locus_tag="O0293_001887"
     CDS             3112..3627
                     /gene="lacB"
                     /locus_tag="O0293_001887"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HCY0280769.1"
                     /translation="MKIALGCDHIVTDTKMRVSEFLKSKGHEVIDVGTYDFTRTHYPI
                     FGKKVGEQVVSGNADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSALYAKEELNAN
                     VIGFGGRIIGELLMCDIIDAFINAEYKPTEENKKLIAKIKHLETSNADQADPHFFDEF
                     LEKWDRGEYHD"
     gene            3640..4572
                     /gene="lacC"
                     /locus_tag="O0293_001888"
     CDS             3640..4572
                     /gene="lacC"
                     /locus_tag="O0293_001888"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HCY0280770.1"
                     /translation="MILTLTLNPSVDISYPLTALKLDDVNRVQEVSKTAGGKGLNVTR
                     VLAQVGEPVLASGFIGGELGQFIAKKLDHADIKHAFYNIKGETRNCIAILHEGQQTEI
                     LEQGPEIDNQEAAGFIKHFEQLLEKVEAVAISGSLPKGLNQDYYAQIIERCQNKGVPV
                     ILDCSGATLQTVLENPYKPTVIKPNISELYQLLNQPLDESLESLKQAVSQPLFEGIEW
                     IIVSLGAQGAFAKHNHTFYRVNIPTISVLNPVGSGDSTVAGITSAILNHENDHDLLKK
                     ANTLGMLNAQEAQTGYVNLNNYDDLFNQIEVLEV"
     gene            4576..5556
                     /gene="lacD"
                     /locus_tag="O0293_001889"
     CDS             4576..5556
                     /gene="lacD"
                     /locus_tag="O0293_001889"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCY0280771.1"
                     /translation="MSKSNQKIASIEQLSNNEGIISALAFDQRGALKRMMAKHQTEEP
                     TVAQIEQLKVLVAEELTQYASSILLDPEYGLPASDARNKDCGLLLAYEKTGYDVNAKG
                     RLPDCLVEWSAKRLKEQGANAVKFLLYYDVDDAEEINIQKKAYIERIGSECVAEDIPF
                     FLEVLTYDDNIPDNGSVEFAKVKPRKVNEAMKLFSEPRFNVDVLKVEVPVNMKYVEGF
                     AEGEVVYTKEEAAQHFKDQDAATHLPYIYLSAGVSAELFQETLKFAHEAGAKFNGVLC
                     GRATWSGAVQVYIEQGEDAAREWLRTTGFKNIDDLNKVLKDTATSWKQRK"
     gene            5578..5889
                     /locus_tag="O0293_001890"
     CDS             5578..5889
                     /locus_tag="O0293_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCY0280772.1"
                     /translation="MNREEVQLLGFEIVAFAGDARSKFLEALTAAQAGDFAKADALIE
                     EGNNCIAEAHRAQTSLLAKEAQGDDIAYSVTMMHGQDHLMTTILLKDLMKHLLEFYKR
                     G"
     gene            5895..7607
                     /locus_tag="O0293_001891"
     CDS             5895..7607
                     /locus_tag="O0293_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCY0280773.1"
                     /translation="MMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSI
                     FLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAFLVAGTTAKSLTDSFNRKLESTN
                     QINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKR
                     NITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLF
                     TAADGWIGVTIIFGAFALFWFVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKI
                     ITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVPTFFGVNEPILFGAP
                     LVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWS
                     FVLAITLIVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILA
                     ASGVSDDAAKASNITEQTNVLVLCAGGGTSGLLANALNKAAEEYHVPVKAAAGGYGAH
                     MDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAALDFVQQ
                     QFEN"
     gene            7625..9037
                     /gene="lacG"
                     /locus_tag="O0293_001892"
     CDS             7625..9037
                     /gene="lacG"
                     /locus_tag="O0293_001892"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCY0280774.1"
                     /translation="MTKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWY
                     TAEPASDFYNRYPVDLELSEKFGVNGIRISIAWSRIFPNGYGEVNPKGVEYYHKLFAE
                     CHKRHVEPFVTLHHFDTPEVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNE
                     IGPIGDGQYLVGKFPPGIKYDFEKVFQSHHNMMVAHARAVKLFKDGGYKGEIGVVHAL
                     PTKYPFDPSNPEDVRAAELEDIIHNKFILDATYLGKYSRETMEGVQHILSVNGGKLNI
                     TDEDYAILDAAKDLNDFLGINYYMSDWMRGYDGESEITHNATGDKGGSKYQLKGVGQR
                     EFDVDVPRTDWDWMIYPQGLYDQIMRVVKDYPNYHKIYITENGLGYKDEFIESEKTVH
                     DDARIDYVRQHLNVIADAIIDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQE
                     RYPKKSAYWYKELAETKEIK"
BASE COUNT         3219 a         1355 c         1709 g         2770 t
ORIGIN      
        1 aggagttttc atataagtgt tccataaaag atataaaata taaaaaatta ttaaaagcta
       61 tatccaatca agactttaaa gggttagttc caaatatcaa tcagaatgat aattattctg
      121 aaatatatat aattaacaat actaaaaact taatatatca tttatatgat gatagaggtc
      181 tatggctagc ttttaataat aatgaagatt atgcaagata ttctgaaaaa tataatgatt
      241 taatatcgga atttgataat gaagatatct aattttttaa tattcgtagc ttttattttt
      301 ctacttattt tatgtttatt tttaatctta caaatgacaa accattaaaa gtagcatccc
      361 aacatcaaac aaaaaaacaa ttcatcaaat aaaaatcgct acaaaaccaa gtcattaaac
      421 acgcaataat taaaattttc cactcattat aattctgaat tccaaatgtc gaattccgaa
      481 aaccaaactc caaattccaa aaacgcaact ccaaaattaa aagcatttcc ctaccattcg
      541 ggaaatgctt tttacatact gattactctg tcattaatga ttttacaacg gaaaccatgt
      601 cgtcatgtat gaccaaagta gcgtcgctat cataaggtgt tcgatcttta ttgataataa
      661 ttaaattgtc gcctttaaaa tgtgatatta atcctgcggc aggttgtaca acgagtgatg
      721 aacctagtac aacaagggtg tcagcatgtt caattttatt taatgccctt atgatggtag
      781 gttgatctaa catttcaccg tataatacga tgtccggtcg aatggcacca ccacaattat
      841 cacaatgttt caaagttcta tcaataacat ctgacttcgt ataagattta tgacatacat
      901 tacaataaaa acgatttaac gtgccatgta attcatcaac atgttgactt ccagcgtctg
      961 agtgcaaacc atcgatattt tgcgtgatga cacctaaaga ttgttgatta cgttctaatt
     1021 ttgcaatcca atcatgaacg atattgggca tcgtatcgac aaatagtaag cgcttatggc
     1081 agaaattgat aaaaccttca ggatcatctt ctaaataatc acggcttaac aagtattctg
     1141 gcgaaagccc atcttttgaa atttcatcaa ataagccacc cattgaacgg aaatctggaa
     1201 cgccacttgc gacagataca ccagcacctg taaaaaatgt aatacgattc gaactatcta
     1261 taatatgttt tagtgtctct aaatcatgtt tcaagttaaa caccccatca aaataatgaa
     1321 tgatttataa cgtcttttac ttttattata gtatattcat tttacgatta tcaaaagcgc
     1381 agtttaaaca gttcttttta aaaatgaatt atacatataa tcgaacagtt aaattatatt
     1441 agtttttacg caatttttat tatagaataa gcatgtatat taaaattgat gtctaaacat
     1501 aaaggataat gacccatcgc ctaaataaag gaggcaattt tacattgaat aaacatgaac
     1561 gtttggacga aattgctaaa ctagtgaata aaaagggcac gataagaacg aatgaaatcg
     1621 tcgaaggttt aaatgtgtct gatatgacag ttcgaagaga tttaattgaa ttggaaaata
     1681 aagggatttt aacgaagatt catggtggtg cacgcagtaa ttcaacattt cagtataaag
     1741 aaatatcgca taaagaaaaa catacccgtc aaattgcaga aaaacgattt atagctaaaa
     1801 aagctgcatc attaattgaa gatggggata ctttgttttt cggaccagga acaacagtgg
     1861 aactattagc agaagaagtc aatcatcata cgctcacaat tattacgaat tgtttgccgg
     1921 tgtataaaat tttgttagaa aaacaaacag cacattttcg tgtctattta attggtggtg
     1981 aaatgcgcca tattacagaa gcatttgtag gtgaaatggc gaacgccatg ttggaaaaac
     2041 taagatttag caagatgttc tttagtagta acgcagtaaa taaaggtgct gtgatgacat
     2101 ctacattaga tgaagcctat acgcaacaac tggcactaag taattcaatt gaaaaatact
     2161 tgttaatcga tcatacgaaa gttggcaaag aagattttac atcattttgt cagttaaacg
     2221 aattgactgc agtggtcatg gactatgaag atgaagaaaa agtagaaacg attaaaacat
     2281 atattgaagt agtcgactaa attaaaagta taactgcatt gttaaaatat gtatctatat
     2341 gctcataagt gaatgtgaat ttgcattcat ttatgggcta ttttttatgc aaatttatgt
     2401 caacgggaac ctcgacaaag ttttactaca atttcagacg aatacctaat taaaacaaca
     2461 ccacaatatg aatgttcact tattgtttga aaatacgtca taaacaaaca tattataaac
     2521 atattatgtt gatttattgt ttgtttatgt ttataattta aacgtaatca aattgaaagc
     2581 cttttctcaa gattttcata acctacaaat aaatatgtca cattttaaga aaggtatttc
     2641 aaaattaaag taaaaaggag tcttattatg gcgattatta ttggttcaga tgaagctggc
     2701 aaacgattaa aagaagtcat caaatcatac ttattagaca ataaatatga tgttgttgac
     2761 gtaacagaag gacaggaagt tgactttgtt gatgcaactt tggctgtagc aaaagatgtt
     2821 caaagtcaag aaggtaactt aggtattgtt atcgatgcat ttggtgcagg tagctttatg
     2881 gttgctacaa aaatcaaagg catgattgct gctgaagtat cagatgaacg ctctggatat
     2941 atgactagag gccataacaa ttcaagaatg atcactatgg gatctgaaat tgttggagac
     3001 acattagcga aaaatgtcgt taaaggtttt gttgaaggta aatacgatgg tggtagacac
     3061 caaatccgcg tagatatgct taataaaatg tgctaaggag gaaaaattac tatgaagatt
     3121 gcattaggat gcgaccatat tgttacagat acaaaaatgc gtgtatctga atttttaaaa
     3181 tcaaaaggac atgaagtcat tgacgtagga acatacgatt tcacaagaac acattatcca
     3241 atttttggta aaaaagttgg cgaacaagtt gttagcggta atgcagactt aggtgtttgt
     3301 atttgtggaa caggtgttgg tattaacaat gctgtaaata aagtacctgg cgttcgttca
     3361 gcactagtac gtgatatgac atcagcgtta tacgctaaag aagaattaaa tgcgaacgtt
     3421 attggcttcg gtggacgtat tataggtgag ttattaatgt gcgatattat cgatgcattt
     3481 attaatgctg aatataaacc aactgaagag aacaaaaaat taatcgctaa aattaaacat
     3541 ttagaaacaa gcaatgcaga tcaagctgat ccacatttct ttgatgaatt cttagaaaaa
     3601 tgggacagag gcgaatacca cgattaaggg acgacacata tgattttaac tttaacatta
     3661 aatccatccg ttgatatttc atacccatta acagcattga aattagatga tgttaatcgt
     3721 gttcaagaag taagtaaaac agctggtggt aaaggtttaa atgtaacaag agtacttgcg
     3781 caagtaggtg agccagtcct tgcgagtggt tttattggcg gtgaattagg tcaatttatt
     3841 gctaaaaaat tagatcatgc cgacatcaag catgcatttt ataacattaa gggtgaaaca
     3901 cgaaactgca ttgcaatttt acatgaaggg caacaaacag aaattttaga gcagggaccg
     3961 gagattgata atcaagaggc tgcaggattt attaaacatt ttgaacaatt attagaaaaa
     4021 gttgaagcag ttgctatttc aggtagctta cctaaaggat taaatcaaga ttattatgca
     4081 caaatcattg aacgatgcca aaacaaaggt gtgcctgtga tattagattg ctcgggtgca
     4141 actttgcaaa cagttttgga aaatccatac aaacctacag tcattaaacc aaacatttct
     4201 gaactatatc aattgcttaa ccagccactt gatgaaagtt tagaaagttt gaaacaggca
     4261 gtgtcacaac ccttattcga agggattgag tggattattg tgtctttagg tgcacaaggt
     4321 gcattcgcta agcacaatca tacattctat agagtgaata ttccgacaat aagtgtatta
     4381 aatccggttg gttcaggtga ttctacagtt gctggaatta cttcagcaat tcttaatcat
     4441 gaaaacgacc acgatttact aaaaaaagcc aatactttag gcatgctgaa tgcacaagaa
     4501 gcacaaacag gttatgtcaa tttaaataat tatgatgatt tattcaatca aattgaagtt
     4561 ttagaggtgt aaaaaatgtc aaaatcgaat caaaaaatcg catcaattga acaattaagt
     4621 aacaacgaag gtatcatttc tgcattagca tttgaccaac gtggtgcttt aaaacgcatg
     4681 atggcaaaac atcaaactga agaaccaact gtagctcaaa ttgaacaatt aaaagtatta
     4741 gttgcggaag aattaacaca atatgcatct tcaattttat tagatcctga atatggttta
     4801 ccagcttcag atgcacgtaa caaagattgt ggattattac ttgcttatga aaaaactgga
     4861 tatgacgtaa acgctaaagg tcgtttacct gactgtttag tagagtggtc tgcaaaacgt
     4921 ttaaaagaac aaggcgcaaa tgctgttaaa ttcttacttt actatgatgt tgatgatgct
     4981 gaagaaatta acattcaaaa gaaagcatac attgaaagaa tcggttcaga atgtgttgca
     5041 gaagatattc cattcttttt agaagtatta acatacgatg acaacattcc tgataacggt
     5101 agtgttgaat tcgcaaaagt taagcctaga aaggttaacg aagcaatgaa attattctca
     5161 gaaccacgct ttaacgttga tgtattgaaa gttgaagtac cagtaaacat gaaatatgta
     5221 gaaggctttg ctgaaggtga agtggtttat acaaaagagg aagcggcaca acactttaaa
     5281 gatcaagatg cagcaactca tttaccatac atttacttaa gtgctggtgt ttctgcggaa
     5341 ttattccaag aaactttaaa atttgctcac gaagctggtg ctaaattcaa cggggtactt
     5401 tgtggccgtg ctacatggtc tggtgctgtt caagtataca ttgaacaagg tgaagatgct
     5461 gctcgtgaat ggttacgtac aactggtttc aaaaatattg atgacttaaa taaagtatta
     5521 aaagatactg cgacatcttg gaaacaaaga aaataagttt aaaaggggat taatgctatg
     5581 aatagagaag aagtccaatt attaggtttt gaaattgttg catttgcagg ggatgcacgt
     5641 tctaagtttt tagaagcatt aacagcagct caagctggag attttgcaaa agcagatgca
     5701 ctgattgaag aaggaaacaa ttgcattgcg gaagcgcata gagcacaaac aagcctgttg
     5761 gctaaagaag cgcaaggtga tgatattgca tacagtgtaa cgatgatgca tggtcaagat
     5821 catttaatga caacaatttt actgaaagat ttaatgaagc atttattaga attttataaa
     5881 agagggtgat gacaatgatg caaaaattga tcgcacaaat tgaaaaaggg aagccatttt
     5941 tcgagaaatt atcacgtaac atatatttaa gagccatccg tgatggattt atttctgcaa
     6001 tgcctgtcat cttattctca agtatctttc tattaattgc atatgtgcca aatatatttg
     6061 gttttaaatg ggataaaggc atggaagcca ttttaatgaa accatacaac tatacgatgg
     6121 ggcttgtagc atttttagta gcaggaacga cagcgaaatc tttaacagat tcatttaacc
     6181 gtaagctaga aagtacaaat caaattaact ttatttctac aatgctagca gcgatgtgtg
     6241 gattcttgtt cttagcttct gatccagcca aagatggtgg attcttaagt gcatttatgg
     6301 gaactaaagg tttattaaca gcattcttat cagcatttgt aactgttatt gtttataact
     6361 tctgtgtgaa gcgcaatatt acaattaaaa tgccgaaaga agtaccgccg aatatttcac
     6421 aagtatttaa ggacttaatt ccattttcag cggtaatcat cattctttat gcattagatt
     6481 tagtcattcg caacagcttt aaatcaaatg tagcggaagg tattttaaaa ttattcgaac
     6541 cattatttac agcagcagat ggatggattg gtgtcacaat tatctttggt gcctttgcat
     6601 tattctggtt tgtaggtatt catggtccgt caattgtaga gccagcaatt gcagccatta
     6661 catatgcgaa tatcgaagcg aacttcaagt tgcttcaagc tggagaacac gcagataaaa
     6721 ttattacatc aggtacacaa atgtttatcg ttacatttgg aggtacgggt gcaacattgg
     6781 tcgtcccgtt catgtttatg tggatgacga aatctaaacg aaacaaagcg attggacgtg
     6841 catcagttgt accaacattc tttggtgtaa acgaaccaat tttatttggt gcaccactag
     6901 tattgaatcc tgtgttcttt attccatttg tattagcacc aattgttaac gtatggattt
     6961 tcaaactttt cgttgaagtg ttaggaatga atagttttag tgtgaattta ccttggacaa
     7021 caccaggtcc attgggcatt atcatgggta caggttttgg tttatggtca ttcgtactag
     7081 ctattacttt gattgttgta gatattatta tttactaccc attcctaaaa gtttatgata
     7141 gtgaaattct tgatgaagaa gaaggacgta aagaaagtaa ttcagattta aaagaaaaag
     7201 ttgcagcaaa ctttgatacg aaaaaagctg attcaatttt agcggcaagt ggtgtatcag
     7261 acgatgcagc taaagcttca aatatcacag aacaaacaaa tgtcttagta ctttgtgccg
     7321 gtggtggtac aagtggatta ttagccaatg cacttaataa agcagctgaa gaatatcatg
     7381 tgccagtgaa agcggcagct ggcggatatg gcgcacatat ggacattatg aaagagtacc
     7441 aattaatcat tttagcgcca caagttgcgt ctaactatga agatattaag caagatacag
     7501 atagattagg tattaaatta gcgaagactc aaggtgctga atatatcaaa ttgacacgag
     7561 atggccaagc agcccttgat ttcgttcaac aacaatttga aaattaagaa taggagtttt
     7621 tcatatgact aaaacattac ctgaagattt tatttttggt ggagcaacag cagcatatca
     7681 agcagaaggt gcgacaaata cggatggtaa aggtcgcgta gcatgggata cgtatttaga
     7741 agaaaactat tggtacactg cagaaccagc gagtgatttt tataacagat atccagtgga
     7801 tttagaatta agtgaaaaat tcggtgttaa tggtatccga atttcaattg catggtctcg
     7861 tatcttccca aatggatatg gcgaagttaa ccctaaaggt gttgagtatt accataagtt
     7921 atttgcagag tgtcataaac gtcatgttga accatttgta acattgcatc attttgatac
     7981 accagaagtg ttacacaaag atggcgattt tctaaatcgt aaaacgattg actactttgt
     8041 agattatgct gaatattgtt ttaaagaatt cccagaagta aaatattgga ctacattcaa
     8101 tgaaattgga cctattggtg atggtcaata tttagtaggt aaattccctc caggtattaa
     8161 atatgatttt gaaaaagtat tccaatcaca tcataacatg atggtggctc atgctagagc
     8221 agtaaaatta tttaaagatg gcggatacaa aggagaaatt ggtgttgtac acgcattgcc
     8281 aacaaaatat ccgttcgatc catcaaatcc tgaagatgtt cgtgcagcag aattagaaga
     8341 tatcatccat aataaattca tcttagatgc aacatattta ggtaagtatt ctcgtgaaac
     8401 gatggaaggt gtgcaacata tcttatctgt taatggcggt aaattaaaca ttacagatga
     8461 agattatgcc attttagatg cagccaaaga tttaaacgac ttcttaggta tcaattacta
     8521 catgagtgat tggatgagag gttacgatgg agaatctgaa atcacgcata atgcgacagg
     8581 tgataaaggc ggttctaaat accaacttaa aggtgtcgga caacgtgaat ttgatgtcga
     8641 cgtacctcgc acagactggg actggatgat ctatcctcaa ggtttatatg atcaaatcat
     8701 gcgtgttgtt aaagattatc ctaactatca taagatttat atcactgaaa atggtttagg
     8761 atataaagat gaatttattg aatctgaaaa aacagttcat gatgatgcac gtattgatta
     8821 tgtaagacaa catttgaacg ttattgcaga tgctattata gatggtgcaa atgttaaagg
     8881 ttactttatt tggtcattaa tggatgtgtt ctcttggtca aatggttatg aaaaacgata
     8941 tggtttattc tatgtcgatt ttgagactca agaacgttat cctaagaaaa gtgcttattg
     9001 gtacaaagag ttagccgaaa caaaagaaat taaataatta tagatattaa aag
//
LOCUS       DAKTIO010000109         8981 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.109, whole genome shotgun sequence.
ACCESSION   DAKTIO010000109 DAKTIO010000000
VERSION     DAKTIO010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8981
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.109"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            98..1231
                     /locus_tag="O0293_001893"
     CDS             98..1231
                     /locus_tag="O0293_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HCY0280775.1"
                     /translation="MINEQRLLNTFFELVQIDSETGNESTIQPILKEKFIALGLDVKE
                     DEAAKHPKLGANNLVCTMNSTIEEGEVPKLYLTSHMDTVVPAINVKPIVKDDGYIYSD
                     GTTILGADDKAGLAAMLEVLQVIKEQQIPHGQIQFVITVGEESGLIGAKELNSELLDA
                     DFGYAIDASADVGTTVVGAPTQMLISAKIFGKTAHASTPKEGVSAINIAAKAISRMKL
                     GQVDEITTANIGKFHGGSATNIVADEVILEAEARSHDPERIKTQVKHMTDVFETTASE
                     LGGKAVVTVEQSYPGFKINDNEAVVKIAQESARNLGLSANTIISGGGSDGSIINTFGI
                     PSVILGVGYEKIHTTNERMPIKSLNLLASQVLEIIKIVARHSK"
     gene            1299..2705
                     /gene="gndA"
                     /locus_tag="O0293_001894"
     CDS             1299..2705
                     /gene="gndA"
                     /locus_tag="O0293_001894"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCY0280776.1"
                     /translation="MTQQIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEE
                     SKGKNIHPTYSLEEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNT
                     NYQDTIRRNKALAQSAINFIGMGVSGGEIGALTGPSLMPGGQEEAYNKVADILDAIAA
                     KAKDGASCVTYIGPNGAGHYVKMVHNGIEYADMQLIAESYAMMKELLGMSHEDIAQTF
                     KDWNAGELESYLIEITGDIFMKLDENKEALVEKILDTAGQKGTGKWTSINALELGIPL
                     TIITESVFARFISSIKEERVNASKELNGPKASFDGDKKDFLEKIRKALYMSKICSYAQ
                     GFAQMRKASEDNEWNLKLGDLAMIWREGCIIRAQFLQKIKDAYDNNPGLQNLLLDPYF
                     KNIVTEYQDALRDVVATGVQNGVPTPGFSSSINYYDSYRAADLPANLIQAQRDYFGAH
                     TYERKDKEGVFHTQWIEE"
     gene            2924..3295
                     /locus_tag="O0293_001895"
     CDS             2924..3295
                     /locus_tag="O0293_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0280777.1"
                     /translation="MEKLNQVMLYVDDVEKAKAFWTETLEFVVVSETPLAEDYVAVEV
                     SPTKDAETSLTIMAKEFIEKHSPEVNLGTPSLMFKEKNFDALYSKLNDLGLTGHDIVE
                     MNGQRVFNFQDGQGNYFAVSD"
     gene            3234..3488
                     /locus_tag="O0293_001896"
     CDS             3234..3488
                     /locus_tag="O0293_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280778.1"
                     /translation="MVNVYLIFKMAKVTILQLVTKQSILIFIQTFILIFGLNFSMKVL
                     FLSFYAIVCTNLLTFLESVTKLVLYSGWKRFPMQKLTGGD"
     gene            3489..4508
                     /locus_tag="O0293_001897"
     CDS             3489..4508
                     /locus_tag="O0293_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCY0280779.1"
                     /translation="MVTIKDVALKAGVSPSTVSRVIKGNQRISEATISKVKKVMEELN
                     YFPNTAARTLITNQTYKIGLVLKGSEEPIRLNPFYINVLLGISETCNQHGYGTQTTVS
                     NNMNDLMDEVYKMIKQRMVDAFILLYSKENDPIKQMLIDESMPFIVIGKPTSDIDHQF
                     THIDNDNILASENLTRHVIEQGVDELIFITEKGNFEVSKDRIQGFETIASQNKINYQI
                     IETSNEREVIFNYMQNLHTRLKDPNIKQAIISLDAMLHLAILSVLYELNIEIPKDVMT
                     ATFNDSYLTEIASPPQTCIDIKPRMLGQQAGAAILNILKNKAQDVIELVIIDTELKIR
                     KSTQR"
     gene            4524..6173
                     /locus_tag="O0293_001898"
     CDS             4524..6173
                     /locus_tag="O0293_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCY0280780.1"
                     /translation="MNKQWWKEAVAYQVYPRSFNDSNHDGIGDLPGMIDKLDYLKDFG
                     IDVIWLSPMFKSPNDDNGYDISDYQEIMDEFGTMEDFDRLLKGVHDRGMKLILDLVVN
                     HTSDEHPWFIESKSSKDNPKRDWYIWQDPKPDGSEPNNWESIFNGSTWEYDANTEQYY
                     FHLFSKKQPDLNWGNPEVRDAVFEMMNWWFDKGIDGFRVDAITHIKKTFEAGDLPVPE
                     GKTYAPAFDVDMNQPGIQTWLQEMKDRSLSKYDIMTVGEANGVSPDDADDWVGEENGK
                     FNMIFQFEHLGLWNSGDSHFDVNSYKSVLNRWQKQLENKGWNALFIENHDQPRRVSTW
                     GDDDKYWYESATSHAAVYFLQQGTPFIYQGQEIGMTNYPFESIETFNDVAVKNDYQIV
                     KAQGGDVDALLAKYKDENRDNSRTPMQWDDTLNGGFTNGEPWFPVNPNYKTINVAQQL
                     EDEHSVLQFYKDLIQLRKSNDVYVYGQFDLVDAENSQVFAYTRTLNEKQVLIVGNLTN
                     HEAELTVPFDLSHGEVKLFNYDAKVNLKQLRPYEACVIELN"
     gene            complement(6256..7122)
                     /locus_tag="O0293_001899"
     CDS             complement(6256..7122)
                     /locus_tag="O0293_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCY0280781.1"
                     /translation="MDVIKQIQQAIVYIEDRLLEPFNLQELSDYVGLSPYHLDQSFKM
                     IVGLSPEAYARARKMTLAANDVINGATRLVDIAKKYHYANSNDFANDFSDFHGVSPIQ
                     ASTKKDELQIQERLYIKLSTTERAPYPYRLEETDDISLVGYARFIDTKYLSHPFNVPD
                     FLEDLLIDGKIKELRRYNDVSPFELFVISCPLENGLEIFVGVPSERYPAHLESRFLPG
                     KHCAKFNLQGEIDYATNEAWYYIESSLQLTLPYERNDLYVEVYPLDISFNDPFTKIQL
                     WIPVKQSPYDED"
     gene            complement(7352..8836)
                     /locus_tag="O0293_001900"
     CDS             complement(7352..8836)
                     /locus_tag="O0293_001900"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCY0280782.1"
                     /translation="MSTKNKHIPCLITIFGATGDLSHRKLFPSIFHLYQQDNLDEHIA
                     IIGIGRRDITNDDFRNQVKSSIQKHVKDTNKIDAFMEHVFYHRHDVSNEESYQELLDF
                     SNELDSQFELKGNRLFYLAMAPQFFGVISDYLKSSGLTDTKGFKRLVIEKPFGSDLKS
                     AEALNNQIRKSFKEEEIYRIDHYLGKDMVQNIEVLRFANAMFEPLWNNKYISNIQVTS
                     SEILGVEDRGGYYESSGALKDMVQNHMLQMVALLAMEAPISLNSEDIRAEKVKVLKSL
                     RHFQSEDVKKNFVRGQYGEGYIDGKQVKAYRDEDRVADDSNTPTFVSGKLTIDNFRWA
                     GVPFYIRTGKRMKSKTIQVVVEFKEVPMNLYYETDKLLDSNLLVINIQPNEGVSLHLN
                     AKKNTQGIETEPVQLSYSMSAQDKMNTVDAYENLLFDCLKGDATNFTHWEELKSTWKF
                     VDAIQDEWNMVDPEFPNYESGTNGPLESDLLLARDGNHWWDDIQ"
BASE COUNT         3103 a         1319 c         1667 g         2892 t
ORIGIN      
        1 cctgataaat ctgtgtaacg tcaattactg taacatttta tttcatcaat ttttaccgtt
       61 tggcgtacat gaaaattttg ataaaagggg ttccattatg attaatgagc aaagattact
      121 taatacgttt tttgaacttg tacaaatcga ttctgagaca gggaatgaat caacaatcca
      181 acctatttta aaagaaaaat tcatagcatt agggttagat gttaaagaag atgaagcggc
      241 taaacatcct aaattaggtg ccaataatct agtgtgtaca atgaatagta ctatcgaaga
      301 aggcgaagta cctaaattat atttgacgag ccatatggat actgttgtac ccgcaattaa
      361 tgtaaagcca attgtaaaag atgacggcta catatactct gatggtacga ctattctagg
      421 tgcagatgat aaagcaggat tagcagcgat gcttgaagtt ttgcaagtga taaaggaaca
      481 acaaatccca catggacaaa ttcaatttgt gattactgtt ggagaagaat caggtttaat
      541 tggtgctaaa gaattaaatt cagagttgtt ggacgcggac tttggttatg ctattgatgc
      601 aagtgctgat gtcggtacta cggttgtagg tgcaccgacg caaatgttaa tttcagctaa
      661 aatttttggc aaaacggctc atgcaagtac gccaaaagag ggtgttagtg ctattaatat
      721 tgcggcgaaa gcaattagtc gaatgaaatt aggacaagtt gatgaaataa ctactgccaa
      781 tattggtaag tttcatggcg gttcagctac gaatattgtt gcagatgaag tcattttaga
      841 agcagaggca cgttctcacg atccagaaag aataaaaaca caagtcaaac atatgactga
      901 tgtgtttgaa actactgcat cagaattagg tgggaaagca gtagttacag tggagcaaag
      961 ttatccagga tttaaaatta atgataatga agcggttgtt aaaattgcac aagaaagtgc
     1021 tcgtaattta ggattatctg caaatacaat tatttctggt ggcggttcag acggcagtat
     1081 tattaataca tttggtattc cttcagtcat tttaggagtg ggttatgaaa aaatccatac
     1141 aacaaatgag agaatgccga ttaaatcatt gaacttgctt gcaagtcaag tgttagaaat
     1201 aatcaaaata gttgcgcgac attcgaaatg atttagtgta gtgtatgtct attttgtgat
     1261 acaatactaa tgaaaaatca acaagtgagg taagtaatat gacacaacaa attggagtta
     1321 ttggtttagc cgttatgggt aaaaacctag cttggaatat tgaatcacgc ggatatagtg
     1381 tatctgtatt taaccgctca agtgaaaaaa ctgatttaat ggttgaagaa tcaaaaggga
     1441 aaaatattca tccaacgtat tcattggaag aatttgttaa ttctttagaa aaaccacgta
     1501 aaattttatt aatggttcaa gcaggtaaag ctacggacgc aacgattgat agtttgttac
     1561 ctttattaga tgatggagat attttaattg atggtggtaa tacaaactat caagatacta
     1621 tcagacgtaa taaagcatta gcacaaagtg caatcaactt tattggtatg ggcgtttctg
     1681 gtggtgaaat tggagcgtta actggtccgt cattaatgcc gggtggacaa gaagaagctt
     1741 ataacaaagt tgcagatatt cttgatgcaa ttgctgcaaa ggcaaaagac ggtgcttcat
     1801 gtgttactta tattggacct aatggtgctg gacactatgt gaaaatggta cacaatggta
     1861 tcgaatatgc tgacatgcaa ttaattgcag aaagttatgc tatgatgaaa gaattattag
     1921 gcatgtctca tgaagacatt gctcaaacgt tcaaagactg gaatgctggt gaattagaaa
     1981 gttatttaat tgaaatcact ggtgatattt tcatgaaatt agatgaaaat aaagaagcat
     2041 tagttgaaaa aattcttgat actgcaggtc aaaaaggtac aggaaaatgg acttcaatta
     2101 acgcattaga attaggtatt ccattaacaa tcattactga atctgtattt gcacgtttta
     2161 tctcttcaat caaagaagag cgcgtaaatg cttctaaaga attaaatggt ccaaaagcat
     2221 catttgatgg tgacaagaaa gactttttag aaaaaattcg taaagcatta tatatgagta
     2281 aaatttgttc ttatgcacaa ggtttcgcgc aaatgcgtaa agcaagtgaa gataatgaat
     2341 ggaatttaaa acttggtgat ttagctatga tttggagaga aggttgcatt atccgtgcac
     2401 aattcttaca aaagatcaaa gatgcttacg ataataatcc aggattacaa aacttattgc
     2461 tagatcctta ctttaaaaac atcgttactg aatatcaaga tgcgttacgt gatgttgtcg
     2521 caactggtgt tcaaaatggt gtaccaacac ctggtttctc atcaagtatt aattactatg
     2581 atagttaccg tgctgctgat ttacctgcaa acttaattca agcacaacgt gactacttcg
     2641 gtgctcatac gtacgaaaga aaagataaag aaggcgtatt ccatacacaa tggattgaag
     2701 aataatattg attaaaggat agttgaaaga tactatcctt ttttatatct tttgataaat
     2761 ataaaagaga gtgtgataga gtagctacaa cctataaaaa attacacaag tattatcctt
     2821 gaccttaaaa gcatcccgcc atatttttaa gcattttgtg aagtaataaa atatttactg
     2881 tgttatgata gcatgtacta tagagaaata gaggtactat tttatggaaa agttaaatca
     2941 agttatgtta tatgttgatg atgtcgaaaa agcgaaagca ttttggacag aaactttaga
     3001 atttgttgtt gtaagtgaaa caccgttagc tgaggactat gttgcagttg aagtatcacc
     3061 taccaaagat gctgaaacgt cattgacgat tatggcaaaa gaatttattg aaaagcatag
     3121 cccggaagtg aacctaggaa caccatcttt aatgtttaaa gagaaaaact ttgatgcatt
     3181 atactcaaaa ttaaatgatt taggtttaac tgggcatgat atagtagaaa tgaatggtca
     3241 acgtgtattt aattttcaag atggccaagg taactatttt gcagttagtg actaaacaat
     3301 cgattttaat atttatacaa acctttatat tgattttcgg attgaatttc agtatgaagg
     3361 ttttattttt atcgttttat gcaatcgttt gcacaaatct tttgactttt ttagaaagtg
     3421 tgactaaact ggtattatac agtggttgga agcgttttcc aatgcagaaa ttaacaggag
     3481 gtgattaaat ggttacgatt aaagatgttg cactaaaagc cggtgtttct ccttcgacag
     3541 tttcaagagt tataaaagga aatcaacgta ttagcgaagc gacaatttca aaagtgaaga
     3601 aagttatgga agaattgaat tattttccta ataccgctgc tagaacttta attacaaacc
     3661 aaacatataa aattggttta gtgttaaaag ggtctgagga gcctattcga ctgaatccat
     3721 tctacatcaa tgtattgcta ggaatttctg aaacgtgtaa ccagcatggc tatggtacac
     3781 aaacgacagt ctcaaataat atgaatgatt taatggatga agtttataaa atgattaaac
     3841 aacgaatggt tgatgcgttt atactgctct attcaaaaga aaatgatccg attaaacaaa
     3901 tgttaattga tgaaagcatg ccatttattg tgattggtaa gcctacatcg gatatagatc
     3961 atcaatttac acatatagat aatgataata tattagcttc tgaaaatttg acacgacatg
     4021 ttattgaaca aggtgtagat gaattaatat ttattacaga aaaaggaaat tttgaagttt
     4081 caaaagatag aattcaagga tttgaaacga tagcttctca aaataaaatt aattatcaaa
     4141 ttattgagac tagtaatgaa agagaagtta tttttaatta catgcaaaat ctacatacgc
     4201 gtttgaagga cccaaatatt aaacaggcaa tcatttcgtt agatgctatg ttacatttag
     4261 cgattttaag tgtcctatat gaacttaata ttgaaattcc gaaagatgta atgacagcaa
     4321 cgttcaatga ttcttattta accgaaattg cgtcaccacc acaaacctgt attgacatca
     4381 agcctcgaat gttaggtcaa caggctggtg cagcaatttt aaatatattg aaaaacaaag
     4441 cacaggatgt tattgaactc gtcattatag atacagaatt aaaaataaga aaatcaacac
     4501 agcgatagca aaggagtttt aaaatgaata agcaatggtg gaaagaagca gtagcatatc
     4561 aagtatatcc aagaagtttt aatgatagta atcacgatgg tattggggat ttacctggaa
     4621 tgattgataa attggactac ttaaaagatt tcggtatcga tgtcatttgg ctcagtccaa
     4681 tgtttaaatc acctaatgat gacaatggtt atgatattag tgactaccaa gagattatgg
     4741 atgaatttgg aacgatggaa gactttgatc gtttattaaa aggtgttcat gatagaggca
     4801 tgaagcttat tttagattta gttgtaaatc atacatctga tgaacatcct tggtttatag
     4861 aatccaaatc tagtaaagac aatcccaaac gtgattggta catttggcaa gatccaaagc
     4921 cagatggctc tgaacctaac aactgggaaa gtatatttaa tggatctaca tgggaatatg
     4981 atgctaatac tgagcaatat tatttccatt tattcagtaa aaaacaacct gatttgaatt
     5041 ggggtaatcc ggaagttaga gatgctgtat ttgaaatgat gaactggtgg tttgataaag
     5101 gcattgatgg atttagagta gatgcaatta cgcatattaa gaagacgttt gaagcgggtg
     5161 acttacctgt acctgagggt aaaacatatg ccccagcatt tgatgtagat atgaatcagc
     5221 caggtataca aacttggtta caagagatga aagatcgctc attaagtaag tatgacatta
     5281 tgactgttgg tgaagcgaat ggtgtaagcc ctgatgatgc tgatgactgg gtcggggaag
     5341 aaaatggtaa atttaatatg atattccaat ttgaacattt gggactgtgg aatagtggtg
     5401 attctcactt tgatgtaaat tcgtataaat ctgtattaaa tagatggcaa aaacaacttg
     5461 aaaataaagg ttggaatgcg ttgtttattg aaaatcacga ccaaccacga cgtgtatcga
     5521 cgtggggtga cgatgacaag tattggtatg aatcagcaac aagtcatgca gctgtttatt
     5581 tcttgcaaca aggtacgcca ttcatttatc aaggtcaaga aattggtatg acgaattatc
     5641 catttgaaag tattgaaacg tttaacgatg ttgctgttaa aaatgactat caaatagtga
     5701 aagctcaagg tggagatgta gacgctttac ttgcgaaata taaagatgag aaccgagata
     5761 attctcgcac accaatgcaa tgggatgata cgttaaatgg aggatttaca aatggtgaac
     5821 cgtggttccc agtgaatccg aattataaaa ctatcaatgt tgcacaacaa ttagaagatg
     5881 agcattcagt attacaattt tataaagatt taattcaatt aagaaagtct aatgatgtat
     5941 acgtatatgg tcaatttgat ttagtagatg ctgaaaattc acaagttttt gcgtacacga
     6001 gaacattaaa tgaaaagcaa gttcttattg taggtaatct tactaaccac gaagcagaat
     6061 taactgtacc atttgattta agccatggag aagtaaagct atttaattat gatgccaaag
     6121 ttaatttaaa acagttacgt ccatatgaag catgtgttat cgaactaaat taacatatta
     6181 atatgccccc taatagaatc taaggccatt tgtagaccta tagatatatt agggggcttc
     6241 tttgtttttt attatttaat cttcgtcata aggactctgt ttaacaggaa tccaaagctg
     6301 aattttagtg aatgggtcat taaatgaaat atcgagaggg tacacttcaa catataaatc
     6361 atttcgttca tatggcaatg ttaactgcaa acttgattca atatagtacc aagcttcatt
     6421 agttgcataa tcaatttcac cttgtaaatt gaatttcgca caatgtttgc caggtaaaaa
     6481 tcgactttct aagtgtgcag gataacgttc acttggtaca cctacaaata tttctaaacc
     6541 attttcaaga ggacaactaa taacaaatag ttcaaatgga ctaacgtcat tatatcgtcg
     6601 taactcttta attttaccat caatgagcaa gtcttctaaa aaatccggaa cattaaaagg
     6661 atgtgacaaa tacttagtgt ctataaatcg tgcatatcca accaatgaaa tatcatctgt
     6721 ctcttctaat ctgtatggat aaggtgctct ctcagtagtt gataatttga tatataatcg
     6781 ctcttgaatt tgtaattcat cttttttagt agaggcttga ataggtgata cgccgtgaaa
     6841 atcactaaaa tcatttgcaa aatcatttga atttgcatag tgatattttt tagcgatatc
     6901 tacaagtcgt gtagcaccat taatcacatc atttgcagcg agtgtcattt tacgcgcgcg
     6961 tgcataagct tctggagata agccgacaat cattttaaat gattgatcaa gatggtatgg
     7021 cgaaagacca acgtaatcac ttaattcttg caaattgaaa ggctctaata aacgatcttc
     7081 aatataaaca attgcctgtt gtatttgctt gataacgtcc aaaactttca ctccaattaa
     7141 ttcaatcatt tatttttatt ctacattatt tctataaatt atacacccat ttgttcaatg
     7201 attattaaaa tagttttggg cattgtaaaa tataatttca taatatagtc tagaaaaaaa
     7261 gcgaatgata gaacaattga tttacttgat tcgtaatcaa tccttgtcat tcgctcattt
     7321 atttttgttt aacatgtgcg ttttaattca attattgaat atcgtcccac caatggttac
     7381 catcacgagc aagtagtaaa tcactttcta atggaccatt agtacctgat tcatagttag
     7441 ggaattctgg atcaaccata ttccattcat cttgaattgc atcaacaaat ttccatgttg
     7501 attttaattc ttcccagtgc gtgaagttag tggcatcacc tttaagacaa tcaaataata
     7561 gattttcata tgcatctaca gtattcattt tatcttgagc gctcattgag taagacaatt
     7621 ggacaggttc tgtttcgata ccttgtgtat ttttcttagc atttagatgt aaagatacac
     7681 cttcattagg ttggatattg attactaata ggtttgaatc taacagttta tcagtttcat
     7741 agtataagtt cattggtact tctttaaatt caacgacaac ttgaattgtt ttagatttca
     7801 tacgtttacc agtacgaata tagaatggta caccagccca tctaaagtta tcaattgtta
     7861 atttacctga aacaaaggta ggtgtgttag agtcatctgc aacgcgatct tcatcacggt
     7921 atgctttaac ttgtttacca tcgatatagc cttcgccata ttgaccacga acaaagttct
     7981 ttttaacatc ttcagattgg aaatgacgca gtgatttaag tacttttact ttctcagcac
     8041 ggatatcttc actatttaaa ctaataggtg cttccatagc taataatgca accatttgta
     8101 acatgtggtt ttgcaccata tcttttagcg cgccacttga ttcataataa ccaccacgat
     8161 cttcaacacc tagtatttca gaagatgtaa cttggatgtt tgaaatatat ttgttattcc
     8221 ataatggttc aaacatcgca ttcgcaaaac gtaatacctc gatattttga accatgtctt
     8281 ttcctaaata gtggtcaata cgataaattt cttcttcttt aaatgattta cgaatttgat
     8341 tgtttaatgc ttcggctgat tttaaatcac taccgaatgg tttttcgata acaaggcgtt
     8401 taaatccttt tgtatcagta agaccagaag attttagata atcagaaata acgccaaaga
     8461 attgtggtgc cattgctaaa tagaatagtc gattaccttt taattcaaat tggctatcta
     8521 attcattact aaaatctagt aattcttgat agctttcttc attactaaca tcatgtctat
     8581 gatagaagac atgttccata aacgcgtcaa ttttgtttgt atcttttacg tgcttttgaa
     8641 ttgatgattt tacttgatta cggaaatcat cattagtaat gtcacgacgt ccaataccga
     8701 tgatggcaat atgttcatct aaattgtctt gttggtagag atggaatatt gatggaaaca
     8761 acttacgatg gcttaagtca ccagttgcac caaagattgt gattaaacat gggatgtgtt
     8821 tgtttttagt actcaagatt aaaacctcaa ttcttaatta gatatatgat ttattattat
     8881 aagataatcc attcagtagg tcatacaata tgctcgactg tatgcagtgt cttaaatgaa
     8941 atatcgattc atgtatcatg tttaatgtga tacttattaa t
//
LOCUS       DAKTIO010000110         8975 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.110, whole genome shotgun sequence.
ACCESSION   DAKTIO010000110 DAKTIO010000000
VERSION     DAKTIO010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8975
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.110"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(100..210)
                     /locus_tag="O0293_001901"
     CDS             complement(100..210)
                     /locus_tag="O0293_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280783.1"
                     /translation="MIEYDLTIILIPILKVAYPFFKKVEREKWYFSGKEV"
     gene            complement(310..846)
                     /gene="nrdG"
                     /locus_tag="O0293_001902"
     CDS             complement(310..846)
                     /gene="nrdG"
                     /locus_tag="O0293_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HCY0280784.1"
                     /translation="MILLDIKQGQGYIAKIESNSFVDGEGVRCSVYVSGCPFNCVGCY
                     NKASQKFRYGEKYTDEILAEILDDCDHDYISGLSLLGGEPFCNLDITLNLVKAFRARF
                     GNTKTIWVWTGFLYEYLANDCTERRELLSYIDVLVDGLFIQHLFKPDLPYKGSLNQRI
                     IDVQQSLSHARMIEYIVS"
     gene            complement(843..2693)
                     /gene="nrdD"
                     /locus_tag="O0293_001903"
     CDS             complement(843..2693)
                     /gene="nrdD"
                     /locus_tag="O0293_001903"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCY0280785.1"
                     /translation="MNQIEEALTGLISKDPAIVNENANKDSDTFSTMRDLTAGIVSKS
                     YALNHLLPKHVADAHQRGDIHFHDLDYHPFQPLTNCCLIDAKNMLHNGFEIGNANVTS
                     PKSIQTASAQLVQIIANVSSSQYGGCTVDRVDELLSTYARHNEEQHRNIAKQFVKESE
                     IDRYVDQQVTKDINDAIESLEYEINTLYTSNGQTPFVTLGFGLGTDHLSRKIQQAILN
                     TRIKGLGKDRTTAIFPKLVFSIKKGTNFSPQDPNYDIKQLALKCSTKRMYPDILNYDK
                     LVEILGDFKAPMGCRSFLPSWKDAEGHFENNGRCNLGVVTLNLPRMALESAGNMTKFW
                     EIFYERIDVLHDALLYRINRLKDAVPNNAPILYKSGAFNYKLKETDDVAELFKNKRAT
                     ISMGYIGLYETATVFYGPDWETSQEAKAFTLEILKEMKRYQTKWTELYDIWFSIYSTP
                     SESLTDRFCRLDQERFGDIKDITDKGYYQNSFHYDVRKDVTPFEKLDFEKDYPYYASG
                     GFIHYCEYPKLQHNLKALEAVWDYSYDKVGYLGTNIPIDHCYECDYDGDFEATEKGFK
                     CPNCGNDNPKTVDVVKRTCGYLGNPVQRPVIKGRHKEICARVKHMKAPKE"
     gene            complement(2940..4310)
                     /locus_tag="O0293_001904"
     CDS             complement(2940..4310)
                     /locus_tag="O0293_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HCY0280786.1"
                     /translation="MNSDNMWLTVMGLIIIISIVGLLIAKKINPVVGMTIIPCLGAMI
                     LGYSVTDLVGFFAKGLDQVINVVIMFIFAIIFFGIMNDSGLFKPLVKRLILMTRGNVV
                     IVCAMTALIGTIAQLDGAGAVTFLLSIPALLPLYKALNMNKYLLILLLALSAAIMNMV
                     PWGGPMARVAAVLKAKSVNELWYGLIPIQIIGFILVMLFAVYLGFKEQKRIKKAIERN
                     ELPQTQDIDVHKLVEVYERDQDVRFPVKGRARTKSWIKWVNTALTLAVILSMLINIAP
                     PEFAFMIGVSLALVINFKSVDEQMERLRAHAPNALMMAAVIIAAGMFLGVLNETGMLK
                     AIATNLIKVIPAEVGPYLHIIVGLLGVPLDLLTSTDAYYFAVLPIVEQTAGQFGVPSV
                     STAYSMVIGNIIGTFVSPFSPALWLAIGLAEANMGTYIKYAFFWIWGFAIVMLVIAML
                     MGIVTI"
     gene            complement(4632..4727)
                     /locus_tag="O0293_001905"
     CDS             complement(4632..4727)
                     /locus_tag="O0293_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280787.1"
                     /translation="MGGFVNSLIMKDLSDNDMRFKYEYYNREKDT"
     gene            complement(4803..5408)
                     /locus_tag="O0293_001906"
     CDS             complement(4803..5408)
                     /locus_tag="O0293_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HCY0280788.1"
                     /translation="MNMPLMIDLTNKNVVIVGGGVVASRRAQTLNQYVEHMTVISPTI
                     TEKLQNMVDNGVVIWKEKEFEPSDIVDAYLVIAATNEPRVNEAVKKALPEHALFNNVG
                     DASNGNVVFPSALHRDKLTISVSTDGASPKLTKSIMAELEALYPPSYSSYIDFLYTCR
                     QKIKVLDITYNEKQQLLSQIVSQEYLNHDKQAQFLAWLDVR"
     gene            complement(5471..7351)
                     /locus_tag="O0293_001907"
     CDS             complement(5471..7351)
                     /locus_tag="O0293_001907"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HCY0280789.1"
                     /translation="MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDT
                     TSDSNQQQLETEVWQQSQISEEQATSTTYMLQNKEPHIEANQRHVTVLYGSESGNAMR
                     LAEIFSERLSDIGHQVVLMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFL
                     EDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDVLLENLGAERICKRVDCDIDYEED
                     AEKWMADIINIIDTTSEGIQSESVISESIKSAKEKKYSKSNPYQAEVLANINLNGTDS
                     NKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLIFMLGWDPQSPVPINDHGDT
                     VPIVEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAREYVINRDFIDLITD
                     FPTIELQPENMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSV
                     HFAERIKPGDIVPIYLKKNPNFKFPMKQDIPVIMIGPGTGIAPFRAYLQEREELGMTG
                     KTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIMEESKRF
                     NEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAELLLRQMKQQQRYQRD
                     VY"
     gene            7546..7638
                     /locus_tag="O0293_001908"
     CDS             7546..7638
                     /locus_tag="O0293_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280790.1"
                     /translation="MSVINAYSNASLLFFIYLLFIMYQISLSVK"
     gene            complement(7746..8243)
                     /locus_tag="O0293_001909"
     CDS             complement(7746..8243)
                     /locus_tag="O0293_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCY0280791.1"
                     /translation="MNNRNVYDIEVSDYKGLTYKLEAFRGKVILVVNTATECIYSEQL
                     KKLETLFQKYKDRGFVVLSFPNNNFDNRQPGSNEEILKISREKFGVTFPVLAKISVNG
                     NDEHPLFTHLKNEQPGIFGSPIKWNFTKFIIDRQGNVVNRFLPMEDPLDISTNIEILL
                     QESSS"
     gene            complement(8253..8513)
                     /locus_tag="O0293_001910"
     CDS             complement(8253..8513)
                     /locus_tag="O0293_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280792.1"
                     /translation="MIIMRGYNGRYCSWTNTAIQCDYFNKVRQVKKDGKSDRLSNNIN
                     SNIIYRVLEKCITLYNVCSRCKRALILSEHSKKNIFNIKKAV"
     gene            complement(8895..>8975)
                     /locus_tag="O0293_001911"
     CDS             complement(8895..>8975)
                     /locus_tag="O0293_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280793.1"
                     /translation="GGQYYLQTRMLSNVDNAEKIISRNSI"
BASE COUNT         2781 a         1714 c         1247 g         3233 t
ORIGIN      
        1 tacgatttaa cgaatttaac aaattttact agaatggcat ttaagaatat ttatacgtta
       61 ttaacgaata tttatttatt gtaaaacgct accaaaaagt tagacttcct tcccactaaa
      121 ataccacttt tctctttcaa cttttttaaa aaacggatat gcaactttta gtattggtat
      181 caaaatgatt gttaggtcat attctatcaa tatattttta taaagaattg cttttattaa
      241 ctttcaatta tgtacctaac ctaaaaagaa gccaaggcaa cgaatgttac cttgacttct
      301 aatacatatt caactaacta tatattcaat catacgcgca tgcgagagtg attgttgtac
      361 atctataatg cgttgattta aagaaccttt atatggtaaa tcaggtttga ataagtgttg
      421 tataaataga ccatctacta aaacgtcaat gtatgataat aactctcgac gttctgtaca
      481 atcatttgct aaatattcat ataaaaatcc agtccatacc caaattgtct ttgtatttcc
      541 aaaacgtgct cgaaatgctt tgacaagatt taatgtaata tccaaattac aaaatggttc
      601 gccacctaat agacttagcc cagatatata atcatgatcg caatcatcta atatttctgc
      661 taatatttca tcagtgtatt tctcgccata tctgaacttt tgtgaggctt tgttataaca
      721 tccaacacaa ttaaatggac atcctgatac ataaacactg catcttactc cttcaccgtc
      781 aacaaagcta tttgattcta ttttagcaat ataaccttgt ccttgtttaa tgtctaaaag
      841 tatcattctt taggcgcttt catatgtttt actcgtgcgc aaatttcttt atgacggcct
      901 ttaattactg gacgttgaac tggattgcct aggtaaccac atgttcgttt aacgacatca
      961 actgttttag gattatcatt gccacagttc gggcatttaa atcctttttc agttgcttca
     1021 aaatctccat cgtaatcaca ttcataacaa tgatcaatcg gaatatttgt acctaagtaa
     1081 ccaactttgt cataagagta gtcccatacc gcttctagtg ctttcaaatt gtgttgcaat
     1141 ttcggatact cacaatagtg aatgaaacca ccactcgcat aataaggata atctttttca
     1201 aaatctaact tttcaaaagg tgtaacatct ttacgtacat cataatggaa agagttttga
     1261 taatatcctt tatctgtaat gtctttaata tctccaaatc tctcttggtc taaacgacaa
     1321 aaacgatccg ttagcgattc actcggcgta ctgtaaatac tgaaccaaat gtcatataat
     1381 tctgtccatt tcgtttgata acgtttcatt tctttaagaa tttcaagcgt aaatgctttt
     1441 gcttcttgag atgtttccca gtctggacca tagaaaacag tagctgtttc atacaaccct
     1501 atatagccca ttgaaatcgt tgcacgttta tttttaaata actcagcaac atcatctgtt
     1561 tcttttaatt tatagttaaa tgcgccactt ttatataaaa tcggtgcgtt attcggtaca
     1621 gcatctttca aacgatttat acgataaagt aatgcatcat gtaacacatc gatacgttca
     1681 taaaagattt cccagaattt cgtcatatta ccggcagatt ctaatgccat tctaggtaaa
     1741 ttaagtgtaa caacaccaag attacaacga ccattatttt caaaatgacc ttccgcatct
     1801 ttccaacttg gtaaaaatga acgacaaccc attggcgctt tgaaatcacc taatatttct
     1861 acgagtttgt cataatttaa aatatctgga tacatacgtt tcgttgaaca ctttaatgct
     1921 agttgtttaa tgtcatagtt cggatcttgc ggactaaagt tggttccttt tttaattgaa
     1981 aatacaagtt tcgggaaaat cgctgtcgtg cggtcttttc ctaagccttt gatacgagta
     2041 tttaagatag cttgttgaat tttgcgactt aaatgatctg tacctaagcc gaatcctaat
     2101 gttacaaaag gtgtctgtcc attagatgta tataaggtat taatttcata ttctaaactt
     2161 tcaatcgcat cattgatgtc tttagtgact tgttgatcaa cataacgatc aatttcagat
     2221 tctttgacaa attgctttgc gatattccta tgttgttctt cattatgtcg tgcatatgta
     2281 ctaagtaatt cgtcaacgcg gtcaaccgta cagccaccat attgactgct agaaacattg
     2341 gctataattt gtacaagctg cgctgatgca gtttgtattg attttggtga agttacattc
     2401 gcgttgccta tttcaaatcc attatgtagc atatttttag catctattaa acaacagtta
     2461 gttaacggtt ggaatggatg ataatctaag tcgtgaaaat gtatgtcccc tctttgatgt
     2521 gcatctgcaa cgtgctttgg taataaatga tttaatgcgt aagatttaga aacgatacct
     2581 gctgttaaat ctctcattgt tgaaaatgta tcactatctt tgttagcatt ttcgttaaca
     2641 atagcaggat ctttagaaat caaacccgtt aatgcttctt cgatttgatt cattttaccc
     2701 cttcttccta tatattgtgt ctactcatta cgttatacca ctatatatag tattttcaaa
     2761 tttttattag tctgttaact aaaacgtcac aaagttatta acatttagtg aaattcatat
     2821 ttctacacaa ttatttcatt aatttttaaa attgtttaaa gattcgtccg tcatttcgac
     2881 atgtttatgc aactattatt tcattttgca acgtgaccat agtttctatt ttttcatact
     2941 taaatcgtca caatgcccat caacattgca attactaaca taacgatagc gaatccccaa
     3001 atccaaaaga atgcatactt aatatacgtg cccatgtttg cctctgctaa accaattgcc
     3061 aaccataagg ctggtgaaaa tgggctgaca aatgtaccta taatattccc tatgaccatt
     3121 gaataagctg ttgatacaga cggtacacca aattgccctg ctgtttgttc gacaatcggt
     3181 aacacagcaa aataataagc gtctgtgcta gttagtaaat ctaatggtac gccaagtaaa
     3241 cctacaataa tatgcaagta tggtcctact tctgcaggaa tcactttgat taaattggtc
     3301 gcaatcgctt taagcatacc ggtttcattt agtacaccta aaaacatacc tgctgcaata
     3361 atcactgcag ccatcattaa tgcattcggc gcatgcgctc ttaatcgttc catttgttca
     3421 tccactgatt taaaattaat aacaagtgcc aacgaaacac ctatcatgaa tgcaaattca
     3481 ggtggcgcaa tatttattaa catcgataga ataacagcta aagttaaagc tgtattcacc
     3541 cattttatcc atgattttgt tcttgcacgt ccttttacag gaaaccttac atcttgatct
     3601 cgttcatata cttcaactaa tttatgtaca tctatatctt gtgtttgcgg taattcattt
     3661 ctctctattg cttttttgat acgtttctgt tctttaaatc caagatatac cgcaaacaac
     3721 ataacaagaa tgaaacctat tatttgaata ggtattaatc cataccataa ttcattgaca
     3781 cttttggctt ttaacactgc agctacacga gccattggac ctccccaagg taccatgttc
     3841 ataatcgccg cgcttaatgc taatagtaaa atcaataaat atttattcat atttaacgct
     3901 ttatataaag gtaataatgc aggaatagaa agcaaaaatg ttaccgcacc ggccccatct
     3961 aattgggcta ttgtgccaat taaagctgtc attgcacaga caatgacgac attgcctcgt
     4021 gtcattaata ttaagcgttt gacaagcggc ttgaataaac cactatcgtt catgatgcca
     4081 aagaaaataa tggcaaagat aaacataata acaacgttga tgacttgatc taacccttta
     4141 gcaaaaaatc caaccaaatc tgtcacacta tatcctaaaa tcattgcccc taagcaaggt
     4201 atgattgtca tacctacaac tggatttatc tttttggcaa tgagtaaacc tacaattgaa
     4261 ataataataa tgagccccat tactgttaac cacatattat cactattcat attattttct
     4321 ccctccactt ttaatacaat ttacataatc cccctcttta acttaaatat tataattaat
     4381 ataatttttt atatttaagt tatctcattt tatcaaaaaa atcttttttg cgtaactatc
     4441 aatagtgaag tttgattttc ctacttgata acatggcatg actaatcctt gtatatcctg
     4501 tcctgtattt cattttgatc tatcattttt attattgctt tctattgcac aaaaaatctg
     4561 gctaaaggtc gctatgatag taacgacttc aaccagattt caatttatcc acctatttta
     4621 ttaagtttgt tctacgtatc tttttctcta ttgtaatatt catatttaaa acgcatatca
     4681 ttatcgctta aatccttcat tattagacta ttcacaaagc cgcccatgtc ttaatttcaa
     4741 tgattaagcg ttaaagttaa aataagaaga cttaccttag acggttagac ggtccgctat
     4801 tattatctta catccaacca cgctaaaaat tgagcttgtt tgtcatgatt taaatattct
     4861 tgtgacacaa tttgtgacag taactgttgc ttttcgttat atgttatatc aagtactttt
     4921 attttctgtc ggcaagtata taaaaagtcg atatacgaac tgtatgatgg tggatataac
     4981 gcctcaagct ctgccataat tgattttgtc aacttaggac tcgcaccatc agttgataca
     5041 ctgatagtta gcttgtcgcg gtgtagtgca cttggaaata caacattgcc atttgatgca
     5101 tctccaacat tattaaaaag ggcatgctca ggtaaggctt tttttaccgc ttcattgaca
     5161 cgtggctcat tggttgctgc aataactaga tacgcgtcta caatatcgct tggttcaaat
     5221 tctttttctt tccatatgac gacaccgtta tctaccatat tttgaagttt ttcagtgatt
     5281 gtcggactga tgaccgtcat atgttcaacg tattgattta atgtttgtgc ccgacgactt
     5341 gcaacgacgc ctccaccaac tatgacgaca tttttatttg ttaaatctat cattaatggc
     5401 atattcatgt tgagcaacat cctttatatc tttttacatc attacacctt aaaatattta
     5461 acaccaatcg ctaataaaca tcacgttgat agcgttgttg ttgtttcatt tgtcgcaata
     5521 ataactctgc ttcttcttga gaaatatgac gttcttttac caatacatct ttaatggctt
     5581 gatggacatc tttcgccata catttttcat cgccacaaat atagattgct gcgccttgct
     5641 caatccattc attgaaacgt ttactttctt ccattatacg atgctgtaca tattctttgt
     5701 gttcttggtc tcttgaaaat gctaaatcta cgcgtgttaa gtttccattt tcaagccatt
     5761 cttctatttc ttcttcatat aaaaagtcag aactacggtg ttgatcacca aagaacaacc
     5821 atgtttttcc agtcatacca agttcttcac gttcttgtaa atatgctcta aaaggagcaa
     5881 ttccagtacc tggtccaatc ataataaccg gtatatcttg cttcatcgga aatttgaagt
     5941 tcggattttt cttcaaataa attggtacta tatcgcctgg tttaattcgc tcagcaaaat
     6001 gaaccgagca tacacctttt ctctcacgtc catgtgcttg ataacgaacc gtaccaacgg
     6061 taatatgcac ttcatctggc gttgccataa aactactaga aatcgaatac tctcttggtg
     6121 gtaattttct aaggatttga tacatattct caggttgtaa ttctatagtt ggaaaatctg
     6181 ttattaaatc tataaagtcc cgatttataa catattcacg cgcccatgac tcatcttgaa
     6241 tacgttcaga taattcttca ttgtcaaaat agatatctgc atttttcaat aatggcaatg
     6301 ttaatttagt aaattcaaaa tgtgatgtta gtgcttcaac aataggaact gtatcaccat
     6361 gatcattaat tggcaccgga gattgcggat cccaacctaa catgaatatt agtttttcaa
     6421 ccaattcagg gtcgttttgc ggtaatgcta ctatacaatc tcctggttca tatgattcac
     6481 taaaatcatc aagtaaaaat tctatatgtc gtgtttcttt atttgaatcg gtaccattta
     6541 aattgatatt cgctaatact tctgcttggt atggatttga tttagaatat ttcttttctt
     6601 tggcagactt aattgattca cttatcaccg attcactttg aataccttct gatgtggtat
     6661 caataatatt aatgatgtct gccatccact tttctgcgtc ttcttcataa tcaatatcac
     6721 aatctacacg cttacatata cgctcagcgc ctagattttc tagtaaaaca tctacatctt
     6781 taccggcttg acagaaaaat tcatatgttt gatcacctaa agctagtact gaatatctca
     6841 catgatttaa attaggtgcg ttatcgtctt ctaaaaattc aaagaaatcc catgcattat
     6901 caggcggttc tccttcacca tgagtagacg taataataaa taaatcttct aactgcgcga
     6961 tgtttgtcgt atcatattca tccattgaca tcaaaacaac ttgatgtccg atatcactta
     7021 aacgttctga aaaaatttca gctaaacgca tggcattacc tgattcagaa ccatatagca
     7081 ctgtaacatg ccgctgatta gcttcgatat gtggctcttt attttgtaac atatacgttg
     7141 ttgaagttgc ttgttcttct gatatttgtg attgttgcca cacttctgtc tctaattgtt
     7201 gttgattact atcacttgtc gtatcgttac tattagctag taaaaaacca ctaagccact
     7261 gttgttggct ctctgttaat gtttgcagca gattgttaat ctctgtgact tgtttttctg
     7321 taaatggact attagatgta tttaatttca aatgttttca cctcaataaa cttgaaatag
     7381 atgattgact ctttttttat cgatagttta gtcaattttt taatatgtat ggtataaatt
     7441 aaaacaaact tgagttgtgt ctgatagtta tacttgcaaa tatttacaac cttcatgttg
     7501 cttgaatgac attatcagta attcagacaa aactttgatt atgtcgtgtc agttattaat
     7561 gcatacagta atgcatcgtt gttatttttt atttatttac tatttattat gtaccaaatc
     7621 agcctttcag taaaataggt caaattactg attttctaaa tataaaatgc ctcctaataa
     7681 catactatta gtacatcatt aagaggctct tgtgttattt gcatactaag cgctcaaatt
     7741 taaatttaag atgaagattc ttgcaataat atttctatat ttgtcgatat atccaatgga
     7801 tcttccattg gcaagaatcg atttacaaca tttccttgtc gatcgattat aaattttgtg
     7861 aaattccatt ttattgggga cccaaagatt cctggttgtt cattctttaa atgcgtaaat
     7921 aacggatgtt catcgttccc gttcacagat attttagcta acactggaaa tgttacacca
     7981 aatttctcac gactaatttt caagatttct tcattagaac ctggttgtcg attgtcaaaa
     8041 ttattattcg gaaaactcaa cactacaaac ccacgatcct tatatttttg aaataaagtc
     8101 tctagttttt tcaattgttc gctatatata cattctgttg cagtattaac aactaaaatc
     8161 actttacctc taaatgcttc taatttataa gttaagcctt tataatcact tacttcgata
     8221 tcatacacat ttctattatt cataagacac ccctacacag ccttttttat attgaatatg
     8281 ttctttttag aatgttctga taaaataagt gcgcgtttac accgtgaaca cacattatat
     8341 agcgtgatac atttttcgag cacacgataa ataatgttcg agtttatgtt gttgctcaac
     8401 ctatccgatt taccgtcttt tttcacctgt cttactttat taaaataatc acattgtata
     8461 gctgtattag tccagctaca atatcttccg ttataacccc tcattataat catccttatt
     8521 ttctattttt aaaaagacaa ttagaccgct ctttaaacta tagattaata cttaagttaa
     8581 actcatacat actgatacca tacgttagat ctaacaattt aaaattcgtt ataactatgg
     8641 attaaagagc tgcccaactc atataatcct taaaaacttc acatgtgatt gtttattaag
     8701 ccctccttta tcttattaaa tatccttata acccttttaa aattaaactg acacactcat
     8761 acattgttac acgcgacgat aaacgtcata ttattgcata tcattaaaac aagtcacttt
     8821 gtatcgtgtg cgaccgaata catattaaat catcaataat gattattcaa ttattaaaaa
     8881 taaaaacgaa cattctatat agagtttcta cttattattt tttcagcatt gtctacatta
     8941 cttaacattc gtgtttgtaa gtaatattga ccgcc
//
LOCUS       DAKTIO010000111         8942 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.111, whole genome shotgun sequence.
ACCESSION   DAKTIO010000111 DAKTIO010000000
VERSION     DAKTIO010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.111"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..256
                     /locus_tag="O0293_001912"
     CDS             <1..256
                     /locus_tag="O0293_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCY0280794.1"
                     /translation="KESRSNTLIELSKILENGYTIDKEEFKKEDDYLSKYIFTDIDYL
                     KKLESKINVAIDIRSGEIHLVLDDDSLDILIQNQKYGNEF"
     gene            299..782
                     /locus_tag="O0293_001913"
                     /pseudo
     CDS             299..782
                     /locus_tag="O0293_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837446.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            943..1047
                     /locus_tag="O0293_001914"
     CDS             943..1047
                     /locus_tag="O0293_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280795.1"
                     /translation="MLLLERTSMSDFEMLMVVLTIIGLVLISTQDHKK"
     gene            complement(1547..2638)
                     /locus_tag="O0293_001915"
     CDS             complement(1547..2638)
                     /locus_tag="O0293_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCY0280796.1"
                     /translation="MFTYFKSAFKNAKPQLLITLIYALIAFAVIAVVYLLANFQLAKY
                     AQTIAIYSQFGQKPPVDAYLKVIAVLLIAAVVSLFVLVQIFIGITNVMKRAMSHEKVK
                     FTDLFIAFKKGNYLKSVLIGLVSIAMIIVLSLLTSLLYKLFSPVSEMIMNSVQSSYAD
                     STHLIGIAITTQSIIIIVVLLIKAIITWLLLIPIFNFMTSFVESTNDKVKTHLANGFK
                     AMKNGQKTFFKFFIGILLLNLIIILFKTPVGYLISFNTQSLSQSVAENIIRVYTVMTI
                     ILFVVIHAIILMGIVQYYLKRGQKITKDKVKTADKNKKVVTEPKNTKVENDKVTTSVE
                     TKTEKAQDSLNDNTTKTMTSDKPEDNQPK"
     gene            complement(2904..3884)
                     /gene="czrB"
                     /locus_tag="O0293_001916"
     CDS             complement(2904..3884)
                     /gene="czrB"
                     /locus_tag="O0293_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc efflux transporter CzrB"
                     /protein_id="HCY0280797.1"
                     /translation="MSHSHHHHDHMHSHVTTNNKKVLFISFLIIGLYMFIEIIGGLLA
                     NSLALLSDGIHMFSDTFSLGVALVAFIYAEKNATTTKTFGYKRFEVLAALFNGVTLFV
                     ISILIVFEAIKRFFVPSEVQSKEMLIISIIGLIVNIVVAFFMFKGGDTSHNLNMRGAF
                     LHVIGDLLGSVGAITAAILIWAFGWTIADPIASILVSVIILKSAWGITKSSINILMEG
                     TPSDVDIDEVITTIKKDSRIQSVHDCHVWTISNDMNALSCHVVVDHTLTMKECELLLE
                     NIEHDLLHLNIHHMTIQLETPNHKHDESIICSGTHSHSHNHHAHHHAHVH"
     gene            complement(3886..4206)
                     /gene="czrA"
                     /locus_tag="O0293_001917"
     CDS             complement(3886..4206)
                     /gene="czrA"
                     /locus_tag="O0293_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)-responsive metalloregulatory
                     transcriptional repressor CzrA"
                     /protein_id="HCY0280798.1"
                     /translation="MSEQYSEINTDTLERVTEIFKALGDYNRIRIMELLSVSEASVGH
                     ISHQLNLSQSNVSHQLKLLKSVHLVKAKRQGQSMIYSLDDIHVATMLKQAIHHANHPK
                     ESGL"
     gene            4358..5023
                     /locus_tag="O0293_001918"
     CDS             4358..5023
                     /locus_tag="O0293_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCY0280799.1"
                     /translation="MNILVIGANGGVGSLLVQQLAKENVPFTAGVRQSDQLNALKSQG
                     MKAILVDVENDSIETLTETFKPFDKVIFSVGSGGNTGADKTIIVDLDGAVKSMIASKE
                     ANIKHYVMVSTYDSRRQAFDDSGDLKPYTIAKHYADDYLRRSGLNYTILHPGALTNAA
                     GSGKIEAAQYFDGKGEIPREDVATVLKEIVTSNHFNHQEFQIISGDQDIKDALTQFEN
                     ETD"
     gene            5458..6396
                     /locus_tag="O0293_001919"
     CDS             5458..6396
                     /locus_tag="O0293_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HCY0280800.1"
                     /translation="MPLFLQPILKTKLWGGQRLSEFGYQLDNDTTGECWCVSAHPNGT
                     SEIINGPYQGQTLDRIWSEHRELFGDFPSKDFPLLTKIVDARESLSIHVHPDNSYAYE
                     HENGQYGKSECWYIIDAEEDAEIVIGTLAESREEVANHVQHGTIESILRYIKVKPGEF
                     YFIPAGTVHTISSGILAYETMQSSDITYRLYDFNRQDNQYNDRPLNIEKALDVIQYNA
                     PLPNILPESEIIENHKCTHIVSNDFFTLVKWEISGTLNYMKPREFCLVTVLEGEGQMI
                     VDGEIFKLTTGTNFILTSEDLDSVFEGDFTLMISYV"
     gene            6427..6657
                     /locus_tag="O0293_001920"
     CDS             6427..6657
                     /locus_tag="O0293_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280801.1"
                     /translation="MKQFLYIALVCGVIAGLGAFLHIPQYPSMTIPRIVAILGIISAM
                     LTFKDKQISASLKFSALLINVLPLCGTFVASN"
     gene            7329..8702
                     /locus_tag="O0293_001921"
     CDS             7329..8702
                     /locus_tag="O0293_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HCY0280802.1"
                     /translation="MTAETNYFWLNCGYNRWNHNEPLVGQTALFESGAHFNPSQGFRA
                     FKKAKVGDQVIFYQVQTDTGLLGCGEIISVETGAQNKIRVQFRFNEQLKPLTADYLKR
                     SEALEFRISNMKETLFNQITAEEFDLISGLGKGEIKIPRYFFLAETEEFEPGNQYTIY
                     THTYNGIKRNGYHFYTQLEEGDNIIFYNRTKNQSVVGIGEVSKHIHEKPPIPGRTNST
                     VIEVSYEKDITPITLSTLNKHPKLKNLYFLQENAKQAIASMSQAQYDAIIEMSDNNGL
                     KSPFEMVQKPDMLESEKEEALKPFILLVVDRKEEGLKAANDLLQKANANPVITTGHPD
                     FSEDMLYGKYLPNETGALYYREGFITQLMPKKDKSYLVIDNFNRIDTDIFQTYINVLE
                     GYEVTLPRYNKDGNMIKWSRQKDSFYYFNPNWHIVGITYDSLEEIKEKYSEQFLKYTR
                     IVKVKHD"
BASE COUNT         3224 a         1198 c         1605 g         2915 t
ORIGIN      
        1 aaaagaaagt aggagtaata ctcttataga gttatcaaaa attttagaga acggatatac
       61 aattgataaa gaagaattta aaaaagaaga tgattatctt tctaagtaca tatttactga
      121 catagattat ttgaaaaagt tagaatcaaa aataaacgtt gctattgata ttcgaagtgg
      181 agaaattcat ttggtattag atgatgatag ccttgatata ttaatacaaa atcaaaaata
      241 cggcaatgag ttttgagtca taactaaata taatatgttg aagagaggtt ttatttaaat
      301 ggctaaaatt ggttatgatc gtgtatcaac gaaagattaa tatcgataca cttaaagaat
      361 ttgggtgtgg gtgtgtatat agtgaaagaa taagtggtgt caaacgtaca gagcttgtta
      421 agtgtttaga ttatttacga gagagcgaca cattagttgt ctatcaactt gatcggttag
      481 gtagaacgac aaaactatta attgaattat cacaatgatt cgatgataac ggaattgact
      541 tacaaattag taacatgaac atttcaacga aagacacaat gggcaaaatg ttttttacga
      601 tgatgagtgc atttttcagg attagaagtt aatttactat gtgagtgtat aaaatagact
      661 tagcagcaac aagagcgaga ggccgaaaaa gcgggcgccc ctctttacca gagaataaaa
      721 aatgagaaat taaattttta tatgatgaac aaacgataac aggggaagaa atagctagtt
      781 agacaagggt atgtcactca actgtttatc gagttgatta agaaatgaaa aaacttatac
      841 tatgaattac tgtttaaaag tgtgcatgtt ataatattta ttgagcaagt tggatagatg
      901 gtggctaatc tcttaataaa ggggtgatgc ctatggttat agttgttact cctagaaagg
      961 actagcatgt ctgattttga aatgcttatg gttgtattaa caatcattgg tttagtattg
     1021 attagtactc aagaccataa aaaataacct tctattcgct ttgaccggca tttttgaagg
     1081 ctatttttaa ataaaatata aggtcaccgt ctttttaacg ggctcattag ggtaacatgt
     1141 ttccgagtgt tgcccttttt gtgtttcaag agttaatgat tttcatcttt tgcttctact
     1201 tactacaaat atattttaac acatttttct tatgaattgt agttctgaac ataatcagaa
     1261 ttaataaaac caactttcca tacagcagaa aatacaatta aaagtataga atgtaatcac
     1321 gaattatata aaaagtattg gagacctctt catatataga taattcactt agttatttta
     1381 gaaagaagcc cctaacaact aaagttgaaa aatagaggaa cacagttgga tgctacgcat
     1441 caactgcata agagccccta acaactaaag tttaaaagta ggagcgcctg cgctaagcgc
     1501 atgcataaga gcccctaaca actaaagttg taaggggctc taaaatttat tttggttgat
     1561 tgtcttctgg tttatctgaa gtcattgttt ttgttgtatt atcatttaat gaatcttgtg
     1621 ctttttctgt ttttgtttca acagatgttg tcactttatc attttctact tttgtatttt
     1681 ttggttcagt gacaactttt ttattcttgt cagctgtttt tactttatct ttagtaattt
     1741 tttgaccacg ttttaagtaa tattgaacga tacccattaa aatgattgca tgaataacaa
     1801 cgaataaaat aattgtcatt actgtataaa ctcttatgat attttctgca acactttgag
     1861 agagtgattg cgtattaaat gaaatcaagt aaccaacagg tgttttaaat aaaataataa
     1921 ttaagtttaa taatagtatt ccaatgaaaa atttgaaaaa tgttttttga ccatttttca
     1981 ttgctttaaa tccattagct aagtgtgttt ttactttatc gttagttgat tcaacgaaac
     2041 tagtcataaa gttgaaaata ggtattaaca ataaccaagt aataattgct ttaatcaata
     2101 aaacaacaat gattatgatg ctttgagttg taatagcaat accaatcagg tgtgtactat
     2161 ctgcatatga tgattgtact gaattcataa tcatttctga tactggagaa aataatttgt
     2221 atagtaatga tgtcagaagt gaaagaacga taatcattgc tatagataca agaccgatca
     2281 gaacgctctt taaataatta ccttttttaa atgcaataaa taaatcggta aattttactt
     2341 tttcatgact cattgctcgt ttcataacat ttgtaattcc gataaaaatt tgaactaaaa
     2401 caaacagtga aactacagct gctataagta atacagcgat aacttttaaa tatgcatcta
     2461 ctggtggttt ttgcccaaat tgtgaataaa ttgcaatagt ttgtgcatac ttagcaagct
     2521 ggaaattagc aagtaaatat acaactgcaa ttacagcaaa tgctataagt gcatatatca
     2581 atgttattaa taattgtggt ttagcatttt taaatgctga tttaaagtaa gtaaacaaag
     2641 tggtgcctcc ttttcttcat ataaatacaa taacaaaaaa cacgttattt tcatattaat
     2701 atctaataaa aattaattaa agtgactaaa gttagagaat tataatagta aattgtcaaa
     2761 aatatgacta atgtaagaaa taatagttga aaatttgtta ataaacattg catcatcaaa
     2821 gaccaaacaa gtcgccataa agacataaaa aacctactgc cattgaaaag tttaatcgat
     2881 gcaatggcag taggttaaaa ttattaatgt acatgcgcgt gatgatgagc atgatggtta
     2941 tgtgaatgac tatgtgttcc tgaacatata atcgattcat catgtttgtg attaggcgtt
     3001 tctaattgaa tagtcatatg gtgaatattt aaatgtaata aatcatgctc aatgttttct
     3061 aataataatt cacattcttt cattgtcaat gtatggtcta caacaacatg acaactcaat
     3121 gcattcatat catttgaaat tgtccaaaca tggcaatcat gcacactttg tattcgtgaa
     3181 tcctttttaa tagtagttat aacttcatct atatcaacat cacttggtgt gccttccatt
     3241 aaaatgttaa ttgaagattt tgtgataccc caagcacttt ttaaaataat aacggaaact
     3301 aaaatacttg cgataggatc ggcgattgtc catccaaatg cccaaattaa aatagctgca
     3361 gtaatggcgc caactgaacc taataagtct ccgataacat gtagaaaagc accacgcata
     3421 tttaaattgt gtgaagtgtc gccgccttta aacataaaga atgcaacaac gatattgaca
     3481 attaaaccga taatactaat gattaacatt tcttttgatt gaacttcaga aggaacaaag
     3541 aaacgtttaa tcgcttcaaa aacaatcaaa atacttatta caaaaagcgt tacaccgtta
     3601 aataacgctg cgagtacttc gaaacgttta taaccaaatg tttttgtagt tgtggcattc
     3661 ttttcagcat aaataaatgc gacaagtgca acacctaatg agaatgtgtc gctaaacata
     3721 tggataccgt cagatagtaa tgccaagctg ttagcaagga gaccgccgat gatttcgata
     3781 aacatatata gaccgattat taaaaacgat ataaacaata ctttcttatt atttgtagtt
     3841 acatgactat gcatatggtc atgatgatga tgtgaatgag acatattata acccactttc
     3901 tttaggatga ttcgcgtgat gtatggcttg ctttaacata gttgctacgt ggatgtcatc
     3961 taatgaataa atcattgatt ggccttgtcg ttttgctttc acaagatgca cacttttaag
     4021 taattttaat tggtgcgaga catttgattg agataaattc aattgatgtg aaatgtgacc
     4081 aacacttgct tcgctgactg ataacaattc catgatacgt attcgattgt aatcgcctaa
     4141 tgccttgaaa atttcagtta cgcgttctaa tgtatctgta tttatttctg aatattgttc
     4201 tgacattata tgacaatcct ttcatgtgaa tatttgttca tatattaatt taatacgttc
     4261 ccagtagtat gtcaattgat ttttatagtt gtaacaagtt ttacatatct cataaagtgg
     4321 tatggcatag agaataataa ataggagagt gttgtaaatg aatattttgg ttataggtgc
     4381 taatggcggt gtaggttcgt tactagtgca acaattagca aaagaaaatg taccatttac
     4441 tgctggtgtt aggcaatcag atcaacttaa tgcgttaaaa tcacaaggta tgaaagcaat
     4501 tcttgttgat gttgaaaatg attcaataga gactttaact gagacgttta aaccattcga
     4561 taaagttatc ttttcagtag gttctggagg gaacacgggt gcagataaaa caattattgt
     4621 cgatttagat ggtgctgtta aatcaatgat tgcgagtaaa gaggccaata ttaaacacta
     4681 tgtcatggtt tcaacatatg attcgagacg tcaggctttt gatgatagcg gtgatttaaa
     4741 gccatataca atagctaaac attacgctga tgactattta agacgttcag gtttgaatta
     4801 tacaatttta catccagggg cacttacaaa tgcagcagga tctggaaaga tagaagctgc
     4861 acaatatttt gatggtaaag gtgaaattcc tagagaagat gttgcgacag tattaaaaga
     4921 aattgtcaca tccaatcatt ttaatcacca agagtttcaa attattagtg gtgatcagga
     4981 cattaaagat gctttaactc aatttgaaaa tgaaacggat taataaggat agtaaatatg
     5041 atttgaagcg ggttactttt tcgtaactcg ttattttttg aaaatgatga attataagta
     5101 tgaataagta cgtattgtct atgcgtttta caaagaattt gaacaatgta tggcacaact
     5161 attaaagttg tttattaatt ggtataatgg gcgagtaaat ttaaagggaa atatgtacaa
     5221 atgtaagaga gatataatct ctatgctata aatttttgaa aatgttaaag tactgtaaat
     5281 ttaagtgaag cgctttaatt ggcagtactg caatgttagt aaatatggta caatcactaa
     5341 tagttaattt gaaatgtgga agttaataat acgatgtaaa tgagaacaaa atcaaaatat
     5401 aatggaacaa caacatcaga gttctattgc ttacttaatc acaatggagg tgcaacaatg
     5461 ccattatttt tacaaccaat tttaaaaaca aaattatggg gcggtcaacg tctaagtgag
     5521 tttggatatc aattagacaa tgatacaact ggggaatgtt ggtgtgtgtc agcacatcca
     5581 aatggtacga gcgagattat taatggacca tatcaaggtc aaacattaga ccgtatttgg
     5641 tcagaacatc gtgaattgtt tggtgatttc ccaagcaaag attttccgct tctaactaaa
     5701 atagtggatg caagagaatc actttctatt catgtgcacc ctgataattc ttatgcttat
     5761 gagcatgaaa acgggcaata tggcaaatct gaatgttggt atattataga tgcagaagaa
     5821 gatgcagaaa tagttatagg gacattagca gagtctagag aagaagttgc gaatcatgtt
     5881 caacacggaa cgatagagtc gatacttaga tatattaaag taaaacctgg agaattctat
     5941 tttattccag caggaacagt acatactatt tcttcaggaa tattagcata cgaaacgatg
     6001 caatcgtcag acattacata tagactttat gatttcaatc gtcaagataa tcaatataat
     6061 gatagaccgt taaatattga aaaagcttta gacgttattc agtacaatgc accattacct
     6121 aatattttgc ctgaaagcga aattattgaa aaccataagt gtacacacat tgtatcgaat
     6181 gatttcttta cattggttaa atgggaaatt tctggcacgt taaattatat gaagcctaga
     6241 gagttctgtt tagttacagt gttggaaggc gaagggcaaa tgattgtcga tggtgaaatt
     6301 ttcaaactga ctactggtac aaactttatt ttgacttctg aagatttgga tagtgtcttt
     6361 gaaggtgatt tcacattgat gattagctat gtgtaatagt gtaataacaa tttaggagtg
     6421 aatgatttga agcaatttct atatattgcg ttagtatgtg gtgtgatagc aggtcttggt
     6481 gctttcttac atataccgca gtatccgagc atgacaattc cacgtatagt agctatttta
     6541 ggaattatca gtgctatgtt gacttttaaa gacaagcaaa tcagcgcctc attaaagttt
     6601 agcgcattgt taattaatgt gctgccatta tgcggtacct ttgtagcttc aaattaattg
     6661 tcgatagcgc gtttgaatat aatacagtgc attaacagaa gaaatatgat tgtgttaatg
     6721 acattgatga aaataaagca catcaacata acctctcact tgcatagtag tgagggggtt
     6781 tttgttggtt tatataaaat gaagtaatta atatttaatg aaaattaaga catgataaaa
     6841 ttctaacggc agtatagata tttaaaaatg tgtatcaatg gcgctataga gataagcaag
     6901 catagtggta tcatatataa atttgtaatt tttattgcat agtgtatgca agcatgtttt
     6961 ttatatgcaa aataggtagc taaatatgtt aaactgtttg taacaatttt tatcattaag
     7021 acagatataa taaataaaat atagaaaact gattaaaagg tatacagttg ctcgtatgtg
     7081 tgtccaactg tatttagagt cgcacttgtt gtaaagcaaa atagttcgac ttacttagaa
     7141 gtgaaaacga taagggtctg ggacgtaaat taatgtcctg ggcgctacaa tgttatagga
     7201 aatgcgatta aaatcgcagt gaacaaaggg tatacagttg ttcgcatacg cgtccaactg
     7261 cataagaaac actaaaagaa tgtaattcat agtgtttcta aaatttattc aggttaagga
     7321 gtattaagat gacagcagaa acgaattatt tttggttgaa ttgtggatat aaccgttgga
     7381 atcataatga accgttagta ggacaaacag ctttatttga atcaggcgca cattttaacc
     7441 catctcaagg atttagagcg tttaaaaagg ctaaagtagg agatcaagtc atcttttatc
     7501 aagttcagac ggatactgga ctacttggat gtggtgaaat tattagtgtt gaaacaggtg
     7561 cacaaaataa aatacgtgta cagtttcgtt ttaatgaaca attgaaacca ttaacagcag
     7621 attatttaaa gcgtagtgaa gctttggaat ttcgtataag taatatgaaa gagacattgt
     7681 tcaatcaaat cactgcagaa gagtttgatt taataagtgg tcttggtaaa ggcgaaatta
     7741 agataccgcg ttatttcttt ttagctgaaa ctgaagaatt tgaacctgga aaccaatata
     7801 cgatttatac gcacacgtac aacggtatta aacgtaatgg ttatcatttt tatactcagt
     7861 tagaagaagg cgacaatatt attttttata atcgaacgaa aaatcaatct gtcgttggaa
     7921 ttggagaagt atcaaagcat attcatgaaa aaccaccaat tcctggtaga acaaatagta
     7981 cagtgattga agtgtcttat gagaaagaca ttacaccgat tacattaagt acattaaata
     8041 agcatcctaa attgaaaaac ttatatttcc ttcaagaaaa tgcaaaacag gctattgcaa
     8101 gtatgtcaca agcacagtat gatgcaatta ttgaaatgag cgataataat ggattgaaat
     8161 ctccatttga aatggttcaa aaaccagata tgcttgaaag tgaaaaagaa gaagcgttaa
     8221 agccatttat tttacttgtt gtagatcgaa aagaagaagg cttaaaagcg gctaatgact
     8281 tattgcaaaa agcgaatgca aatcctgtta taacaacagg tcatcctgat ttttcagaag
     8341 atatgcttta tggcaagtat ttaccaaatg aaacaggggc attatactat cgtgaagggt
     8401 ttattactca attaatgcct aaaaaggata aaagttattt agttattgat aattttaatc
     8461 gtatagatac agacattttc caaacgtata ttaatgtgtt agaaggctat gaagtaacat
     8521 taccacgtta taataaagac ggtaatatga ttaaatggtc acgacaaaaa gattctttct
     8581 attatttcaa tcctaattgg catattgtag gtattacata tgacagttta gaggaaatta
     8641 aggagaaata ttcagagcag tttttaaaat acacacgtat agtcaaagtc aaacatgact
     8701 aatgatatat ggtagttttt gaaaagaagt aaggatagga ttaaaaattg cataattgca
     8761 tatttgaata accgcataaa gaaaaaatga cgggtaatca accataagag ctaattaaga
     8821 gcttatgata gattactcgt ttttatttta ataaataata aagacagtat atgaatataa
     8881 aagaatttat gttacagaat taaaacttta tatgagtttt ccagtggtat gttgaataag
     8941 ac
//
LOCUS       DAKTIO010000112         8868 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.112, whole genome shotgun sequence.
ACCESSION   DAKTIO010000112 DAKTIO010000000
VERSION     DAKTIO010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8868
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.112"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..207)
                     /locus_tag="O0293_001922"
     CDS             complement(<1..207)
                     /locus_tag="O0293_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCY0280803.1"
                     /translation="MTSKDITQISVIAAILTILAVLKIPSIIPGLDFQLSAPAALLIL
                     AFFGIKKYFLGGLLSSLLLLVFGVF"
     gene            complement(218..910)
                     /locus_tag="O0293_001923"
     CDS             complement(218..910)
                     /locus_tag="O0293_001923"
                     /EC_number="6.2.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxyhexanoate--CoA ligase"
                     /protein_id="HCY0280804.1"
                     /translation="MYSIKMRSSNQDVHISGAETICEFDKIEQTVQRFYNKGFFHENG
                     QPDFLNIKIQKIMEPIQQIKALQIIEDDKANLQHLTQECGVTEQALNQGMTYIKNETV
                     YTGAIILSAISGKRLDSFGQRGIRATHFSFEDINNKGDLNERVTDALAIASCINAHPY
                     VKGELCVSDDLTYTTGYFAAAKIGYHRLFDIKPVNTRYGGRIIFVDDCIDLNHYISFL
                     ESTPKQVVYETV"
     gene            complement(921..2036)
                     /locus_tag="O0293_001924"
     CDS             complement(921..2036)
                     /locus_tag="O0293_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HCY0280805.1"
                     /translation="MKFQEQIDLLQQKGLYRSLKSVGYIDRQYIEVENKRCTNYTSND
                     YLGLGQIAFDKDDFERFMRKYSYHLSSSRLISGSSTAYEEIETMLAGWLGYSACTILN
                     SGYDANLALFNIFKNTNCVVFSDQENHASIIDGIKLSGLEKVIYKHLDIADLEKRLEK
                     YPNQNIPKIIISDSVFSTNGDVVDIGQLVSLKHKYNATLILDVSHSFGIENYSNYQGV
                     DILTSSLSKACGAYGGVILSSNDVKDMLINHGRPLIYSSSLPIYNLYFIKRNIEKLIN
                     ADDRRTKLNSLSKYFNQKLKALNVNYNSSNSPIKFIEFDDIEAAENIHQTLLKHHVFT
                     SYLRYPTVTKPMLRISLSYFHTEQDIDRLFEILHQED"
     gene            complement(2014..3021)
                     /gene="bioB"
                     /locus_tag="O0293_001925"
     CDS             complement(2014..3021)
                     /gene="bioB"
                     /locus_tag="O0293_001925"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCY0280806.1"
                     /translation="MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYF
                     GKKVKLNMILNAKSGICPENCGYCGQSRDIKQKQRYALIPEEQIIDGAKVAHDNHIGT
                     YCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYN
                     HNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFAL
                     KEMDADSIPINFLHPIKGTKFGSMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNL
                     RSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYDTCENKENKNEVSRA
                     N"
     gene            complement(3023..4381)
                     /gene="bioA"
                     /locus_tag="O0293_001926"
     CDS             complement(3023..4381)
                     /gene="bioA"
                     /locus_tag="O0293_001926"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCY0280807.1"
                     /translation="MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDT
                     NGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIAHSTLLGSSNIPSIELAEKLIEI
                     TPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAV
                     SVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEMMTAILTNIEQILIERNDEIA
                     GFILEPLIQGATGLFVHPKGFLKEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHED
                     VQPDIMCLGKAITGGYLPLAATLTSKKIYNAFLSDSHGVNTFFHGHTYTGNQIVCTVA
                     LENIRLYEKRKLLSHIETTSSTLEKQLHALKRHRNVGDVRGRGLMFGVELVTDKDSKT
                     PLEIEKVERIVRNCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDR
                     KC"
     gene            complement(4359..5045)
                     /gene="bioD"
                     /locus_tag="O0293_001927"
     CDS             complement(4359..5045)
                     /gene="bioD"
                     /locus_tag="O0293_001927"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HCY0280808.1"
                     /translation="MRIFITSTNTDVGKTYVTKHLYHALKTRGHRVCIFKPFQTEERQ
                     DGTFPDLEVFKNECDLSYDITSLYTFKQPVSPHLAFKMTDQIFLNKQRVLDKVKVLDK
                     EFDFILIEGAGGIAVPIYEGTDDFYMTKDLINDCADCVISVLPSKLGAISDAIVHQDY
                     VNQNVSASNFLIMNRYTDSYIEKDNQMTIGKLTNKTVYTFEEHATYENFSEAFLKQLI
                     GVKNELHTTT"
     gene            complement(5099..5266)
                     /locus_tag="O0293_001928"
     CDS             complement(5099..5266)
                     /locus_tag="O0293_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280809.1"
                     /translation="MQLLILTDIFYRIAVYIWPNSLRSLSHFNSKVKILPKFIVILRS
                     LNSCFLEKHAP"
     gene            complement(5497..7230)
                     /locus_tag="O0293_001929"
     CDS             complement(5497..7230)
                     /locus_tag="O0293_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0280810.1"
                     /translation="MFQITFKILNWIRPYKARMILGFSMSFLNAIFIALPIFLAAKIF
                     NNVLSHKPIYMKDILNVVIIMVLLVIGRFITAYFKSKSHESIAYEMSAKERLDIGDKL
                     KNVRLGYFNSHHSNELTTIVTTDLTFLENFAMKMVDVVVNGYILITVLILSLLVVSWQ
                     VSLLACIGVLLSFFAIQLLERKSRQNAPAYHNVQNQLVEKVLEVIRGIQVIKSFAKEN
                     TSLKSFNQAVNESKRINTKIEMQYIPFNLLHLLSLKVVSIMIVLVACLLYMNHSIDLP
                     TLIMISIFSFVIFDSVENINSAAHVLEMIDMTIDDIEKIKNAPELDENGKNLTIKNEN
                     IAFQNVNFSYDDKQVIKNVNFEIPTQTSTAIIGPSGSGKSTLCHLLLRFYDIDDGNIR
                     IDGVDIKDMTLSTLMSKISAVFQKVYLFNDTIENNILFGNPGATKEEIIRAAKQACCH
                     DFIMSLPEGYQTMLNEKGSNLSGGEKQRISIARAILKDAPIIILDEATASIDPENEQL
                     IQTAINELSKGKTVITIAHKLETIKNADQIIVLNEGEIIQKGSHDELIRKPGMYQDFI
                     RIKSKSAGWKL"
     gene            complement(7255..>8868)
                     /locus_tag="O0293_001930"
     CDS             complement(7255..>8868)
                     /locus_tag="O0293_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0280811.1"
                     /translation="INNTYTINSIMVYIFIALIAYISQVCCFGASTMLSHITAYEILS
                     EIRKKLAQKLMRLPLGVVESKKIGELKNIFVDKVETIELPLAHMIPEVIGNLLLSAAI
                     FLYIMLIDWRMASALLVTIPISIFAFKKVMSGFNETYAEQMKSNNYMNSAIVEFIEGI
                     EVIKTFNQSQSSYKKYKDAVDNYKIHTLNWFKNTWGYMNLGASVLPSTFLGILPVGMY
                     LISINQLNYAEFFLCIVLSLGVVAPIKNFTNYVNHLKSIQYALTEVNQILSLEELVLS
                     TKFKKPQHYEIAFNNVGFSYNKDKDDLVFKHLSFTVPENNFTAIVGASGSGKSTIAKL
                     ISRYWDVTSGEITIGGINIKDIESKQLNDLVGFVGQDNFLLNLTFKENIKLGNPEATD
                     EAVEKAAKLAQCHEFIEKLPDGYDTNVGTVGDKLSGGEKQRVTIARMILKDAPIIVLD
                     EATVYVDPDNEQKIQEALNVLTQDKTLIVIAHRLSTIQHADQIIVLGKQQILEKGSHH
                     LLLKLNGNYKKMWDTHMHTKDWGINTGHN"
BASE COUNT         2804 a         1657 c         1149 g         3258 t
ORIGIN      
        1 aaatacgcca aatactagta ataataggct agataataat ccacctaaaa agtacttttt
       61 aattccaaag aaagctaata tcaataatgc tgccggtgca gataattgaa aatctaatcc
      121 tggtataatg gacggtattt tcaaaactgc caaaatggtt aaaatcgcag caatgacact
      181 aatttgagta atatcttttg atgtcatact aaaaccccta taccgtttca taaacaactt
      241 gcttcggtgt gctttctaaa aatgatatgt aatgatttaa atcaatacaa tcgtccacaa
      301 atattattct gcctccatat ctcgtattaa ctggtttaat atcaaataat cgatggtaac
      361 caattttagc agcggcaaaa taacctgtcg tatacgttaa gtcatcggac acgcaaagtt
      421 ctcctttgac atacggatgc gcattgatac aactagcaat tgctaaggca tcagtcactc
      481 tttcattaag atcaccttta ttatttatat cttcaaacga aaaatgtgtt gccctaatcc
      541 ccctttgtcc aaatgaatct aaacgtttac cagatatagc agatagaata atagctcctg
      601 tataaaccgt ttcattttta atatatgtca tcccttgatt tagcgcttgt tcagtgacac
      661 cacattcttg tgttaaatgt tggagatttg ctttatcatc ctcaataatt tgtaatgctt
      721 ttatttgttg aatcggttcc atgatttttt gtatttttat attgagaaaa tccggttgcc
      781 cattttcatg aaaaaaacct ttattataaa acctttgtac cgtctgttct attttgtcaa
      841 actcacaaat cgtttcagca ccactaatat gaacatcttg attgctagaa cgcattttta
      901 tactatacat gacgatcacc tcaatcttct tgatgcaaaa tttcaaacaa cctatctata
      961 tcttgttcag tatgaaaata cgacaatgat attcttaaca ttggcttagt cacagttgga
     1021 taccttaaat aacttgtaaa cacatgatgc tttaataatg tttgatgaat gttctcagcc
     1081 gcttctatgt catcaaactc aataaactta atcggcgagt ttgaactatt ataattaaca
     1141 ttgagtgctt ttaacttttg gttaaaatat ttactcaaac tatttaattt agtgcgtcta
     1201 tcatcagcat ttattaactt ttcaatgttt ctttttataa aatacaaatt ataaattggc
     1261 aaactacttg agtagatgag tggtctaccg tgattaatta acatatcctt cacatcattt
     1321 gaacttaaaa tcacaccccc gtatgcacca catgctttag ataaactaga agtgagtata
     1381 tctacacctt gataattcga gtaattctct attccaaaac tatgtgaaac atcgagtatc
     1441 agtgttgcgt tatatttatg ctttaatgag actaattgac caatatccac aacgtcgcca
     1501 ttcgttgaaa atacactatc agatatgatt atttttggta tattttgatt agggtatttc
     1561 tctaaccttt tttctaaatc agcaatatct aaatgcttat atatcacttt ttctaaacca
     1621 cttaacttaa taccgtcaat aatactcgca tgattttctt gatctgaaaa cacgacacaa
     1681 tttgtatttt tgaaaatatt aaataacgcc aaattagcat cataaccact atttaagata
     1741 gtacatgcac tatatccgag ccaacctgct aacattgttt caatttcttc ataagctgtc
     1801 gaacttccac taattaatct tgaacttgat aagtgataac tatacttccg cataaatctt
     1861 tcgaaatcat ccttatcaaa cgctatttga cctaatccta aataatcatt agatgtatag
     1921 ttcgtacatc tcttattttc tacttcaata tactgtctat ctatataccc taccgattta
     1981 agcgaccgat ataacccttt ctgttgtaat aaatcaattt gctcttgaaa cttcattctt
     2041 gttttcctta ttttcacaag tgtcataatc aatttcaaag cctaaatcat taatcatatc
     2101 gtagtctaat tggttcggtt gcccaccagt aattagataa tcaccgacaa atattgaatt
     2161 cgccgctttt aatgctaatg gctgtaacga acgtaagttg acctctcttc ctccagcaat
     2221 acgaatttct ttcgtaggat tgattaatcg gaataatgct acgattctta aacatttcat
     2281 tggtgttaaa tcatccatgc ttccaaactt tgtgcctttg attggatgca aaaaattaat
     2341 cggaatactg tcggcatcca tttcttttaa agcaaatgcc atatcaacaa tatcttgatt
     2401 agattctccc ataccacaaa tcacgccaga acatggtgat atattattcg ctttcattag
     2461 ttctatcgta tctgttctat ctttataact atgcgttgtc acgacgttat catggtaatt
     2521 ttcacttgta ttaatattgt ggttatatct gtctacacca gctgacttaa gtttcttagc
     2581 ttgttcgtca ttcgttaatc ctaaacatgc acagattttt agttgcgggt gttgagattt
     2641 aatcgttctt acagtattac taatatgatc aacttcttta tcgctcggtc ctctaccact
     2701 cataacaata caatatgttc caatatgatt atcatgtgcc acctttgctc catcgataat
     2761 ttgttcctct ggaattaaag catatcgctg tttttgttta atatctcgtg attgtccaca
     2821 gtacccacaa ttttcaggac atataccact tttagcattt aaaatcatgt ttaattttac
     2881 ttttttacca aaataatgtt ttcttaaaat gtacgcctca tttaataaat ctaaggtatc
     2941 aatattagta tcctcataaa ttttcaatac agtctctttt gttaattgtt ccccttgtaa
     3001 tatgcgttta gccaaattca tattaacact tcctatctaa aatgttatgt actgcctttt
     3061 taaaaatacg taccatcgtt ttcacttctt tatttgacat acttaacact ggcacaaacg
     3121 taatgacatt ttctaaattt ctaatcatta gcccgttttc tttacaatta cgtacaatac
     3181 gttcaacttt ttcaatttct aacggcgttt tactatcttt atctgtaact aattcaactc
     3241 caaacattaa gcctcgtcct cttacatcac caacatttcg atgacgcttc agcgcatgta
     3301 actgtttctc aagtgttgat gatgtcgttt caatatgtga caataactta cgtttttcat
     3361 aaagtcttat attttctaat gcaaccgtac aaacgatttg atttccggtg tatgtatgac
     3421 catggaaaaa ggtattcaca ccatgcgaat cacttaaaaa tgcattgtat atttttttag
     3481 atgtcaatgt agctgcaagt ggtaagtagc caccagtaat cgccttacct aaacacataa
     3541 tatccggttg aacatcttca tgattgcatg caaacatctt tccagttctc ccaaaaccaa
     3601 ctgctacctc atcacaaatt aataagacat cgtatttttt gcacaatttc tcgacttctt
     3661 tcaaaaagcc tttaggatga acaaacaagc ctgtcgcacc ttgaatcaac ggttccaata
     3721 taaaccctgc gatttcatca tttctttcaa ttagaatttg ctctatattc gttaaaatag
     3781 ctgtcatcat ttcattttca gtatcgtaat tactgcgata gaaagatggg ctttctactt
     3841 gaatattctc aaatattaag tctttaaata ttttatgaaa ggtcttgata ccaccaacac
     3901 ttaccgcacc aatcgtatcc ccatgataac cgtgatttag cgttataaac ttgtttttct
     3961 tggcatattt ttctctatca atatttttcc aatactgata tgccatcttt attgcgattt
     4021 caacagacgc actgcctgta tcagaataaa atacttttct tagattactt ggcgtgattt
     4081 cgattaattt ttccgcaagt tctattgacg gaatatttga tgatcctagc agcgtagaat
     4141 gggcaatttt attgagttgc tttttaatta ccttatttaa gtatttgtta ttatgaccat
     4201 gcacattgac ccacaacgat gcataaccat ctaaatattt attgccattc gtatcgtaaa
     4261 ggtaactacc ctttcctttt tcaatgatga ttgcttcttc tttgctatat acacccattt
     4321 gtgtaaatgg atgccaaaca tattctgagt ctttttgttt aagttgttgt gtgtaattca
     4381 tttttaactc ctattaattg ttttaaaaat gcttctgaga aattttcata cgtggcatgt
     4441 tcttcaaatg tatagactgt tttatttgtt aattttccaa tcgtcatttg attgtctttt
     4501 tcaatatagc tgtctgtata gcgattcatt attaaaaaat tactcgccga tacattctga
     4561 ttaacataat cttggtgaac aatggcatcg ctaatagcac ctaattttga tggcaacaca
     4621 ctgatgacac aatctgcaca atcattgatt agatctttag tcatgtagaa atcatctgta
     4681 ccttcatata ttggtacggc aattccccca gcaccctcaa ttaagataaa atcaaattcc
     4741 ttatctaaaa cttttacctt atctaataca cgctgcttat ttagaaaaat ttgatctgtc
     4801 attttaaatg caaggtgtgg tgatacaggt tgcttaaaag tataaagtga cgttatgtca
     4861 tagcttaaat cacattcatt tttaaatact tctaaatctg gaaacgtccc gtcttggcgt
     4921 tcctcagttt gaaatggttt aaaaatacaa actcgatgac cacgtgtttt caaagcatgg
     4981 tataaatgct ttgtaacata ggttttgcct acatcagtat tcgtacttgt aataaaaatc
     5041 ctcataaaaa taaccacctc aatccaaatg taaactttta taattaataa gtttacattt
     5101 aaggcgcatg tttttcaaga aaacatgaat ttaaacttct cagtataact ataaattttg
     5161 gcaaaatttt gaccttgcta ttgaaatgac taagacttct taaggaattc ggccagatat
     5221 aaacagcgat tcgataaaat atgtcagtta aaattaataa ttgcattatt tcttaataaa
     5281 aagacatcct agctttacca tcttgtgaaa caatccatta attgtaatat tacgttgagc
     5341 gccttgttca tcaatgctat aattagttac ataataaatt gaacatctaa atacaccaaa
     5401 tcccctcact attgccctag tgaggggatt tattttgctg ttgattatct atcacccttt
     5461 ttatttccaa attctgattc catattaaac tattatttat aatttccagc ctgctgattt
     5521 actttttatt cttataaagt cttgatacat tcctggtttt cgaattaatt catcatgact
     5581 acctttttga attatttcac cttcattgag cactataatc tgatctgcat ttttaatagt
     5641 ttcaagttta tgtgcaattg taattactgt tttgccttta cttaattcat taattgccgt
     5701 ttgaatcagc tgttcatttt cagggtcaat acttgcagtt gcttcatcta aaataattat
     5761 tggtgcatct tttaatatcg ccctagcaat agaaatcctt tgcttttctc cgccagataa
     5821 attactacct ttttcattta gcattgtttg atatccctca ggtaatgaca tgatgaagtc
     5881 gtggcaacat gcttgcttcg ctgcacgaat aatttcttct ttcgttgcac ctggattgcc
     5941 aaacaatatg ttattttcaa tcgtatcatt aaataaatac accttttgaa atactgcact
     6001 aattttcgac attaacgtac ttaatgtcat atctttaata tcaacaccat cgatgcgaat
     6061 atttccatca tcgatatcat aaaagcgcaa gagtaagtga cataatgtag atttcccact
     6121 tcctgaaggt ccaattattg ctgttgatgt ttgtgtaggt atctcaaaat tcacattctt
     6181 tatcacttgt ttatcatcat atgaaaagtt cacgttttga aaagcgatat tttcattttt
     6241 aatcgtcaaa ttttttccat tctcatccag ttctggagca ttttttatct tttcaatatc
     6301 atctatcgtc atatctatca tttcaagtac gtgtgcagca ctattaatat tttcaacact
     6361 atcaaatatc acaaatgaaa aaattgaaat cataataagg gtaggtaaat caatactatg
     6421 attcatatat aataagcatg caactaatac aatcattatt gaaacaactt ttaaactaag
     6481 taaatgcaat aaattgaatg ggatatattg catttctatt tttgtattta tacgtttact
     6541 ttcattgact gcttggttaa aacttttaag actcgtattt tctttcgcga atgattttat
     6601 tacttgaata ccacgaataa cctccaatac cttttccact aattggtttt gtacattatg
     6661 atacgctggc gcattttgtc gactctttct ttctaataat tgaatcgcaa aaaatgatag
     6721 taatacgcca atgcatgcta ataatgatac ttgccacgaa actacaagta gagacaatat
     6781 gagtactgta attaatatgt atccattaac aacaacgtcc accattttca tagcaaagtt
     6841 ttctaaaaag gttaaatctg ttgttactat tgttgttaac tcatttgaat gatgcgaatt
     6901 aaaataacct aaccttacat ttttcaattt atcccctata tctaaacgtt ctttcgcact
     6961 catttcataa gcgatgctct catggctttt gcttttgaaa tatgctgtaa taaatcgtcc
     7021 aatcactaat aaaaccatga taattacaac attcaatata tctttcatat aaataggttt
     7081 atgagatagc acattattaa atatttttgc agctaaaaag ataggtaacg caataaaaat
     7141 agcatttaaa aatgacatgc taaatcctaa aatcattctt gctttatatg gtcttatcca
     7201 gtttaaaatt ttaaatgtaa tttgaaacat atttgacctc cttttctcat tagtttaatt
     7261 atgtccagta ttgattcccc aatcttttgt atgcatgtgc gtatcccaca ttttcttata
     7321 attaccgttt aattttagca ataagtgatg tgaccctttt tctaaaattt gttgtttacc
     7381 taaaacaata atttgatctg catgttgaat cgtagataac cgatgtgcaa taacaatcaa
     7441 tgttttatcc tgcgtcaaca catttaatgc ttcttgaatt ttttgttcgt tgtctggatc
     7501 aacgtaaaca gtcgcttcat ctaatacaat gataggcgca tcttttaata tcattcttgc
     7561 aatagtgact cgttgttttt caccaccaga caatttatct cccactgtac caacattcgt
     7621 atcatatcca tctggcaact tttcaataaa ttcatgacat tgtgctaact tggcagcttt
     7681 ttcaactgcc tcatccgtag cttctggatt gccaagttta atattttctt taaaagtaag
     7741 atttaataaa aagttatctt gtccaacaaa accaactaaa tcgttaagtt gtttcgattc
     7801 aatatctttt atatttatac cgccaatcgt aatttcacct gaagtcacat cccagtatcg
     7861 tgatataagc ttggcaatgg ttgacttacc gctaccagat gccccgacga tagctgtgaa
     7921 attattttct ggtactgtaa atgataaatg cttaaagacc agatcgtcct tatctttgtt
     7981 atatgaaaat ccaacattat taaaagcaat ttcataatgt tgaggcttct taaattttgt
     8041 tgacaatact aactcttcta gacttaatat ttgattcact tcagttaatg cgtattgtat
     8101 agactttaaa tgatttacat aattagtaaa attcttaatc ggtgctacta cacctaaaga
     8161 taatacgatg cataggaaaa attccgcata gtttaattgg ttgatagata tcaaatacat
     8221 gccgaccggt aaaatcccta aaaatgttga aggtaataca ctagctccta aattcatata
     8281 cccccatgta tttttaaacc aattcaaagt gtgaatctta taattatcta ctgcatcttt
     8341 atatttttta tatgaacttt gagattgatt aaatgtttta atcacttcaa tgccttcgat
     8401 aaactctaca atcgcgctat tcatataatt attcgatttc atttgttcag catatgtctc
     8461 attaaatcca gacataactt ttttaaaagc gaaaattgaa attggtatcg ttactaataa
     8521 ggcactagcc atacgccaat caatgagcat tatgtataaa aagatagcag ctgacaaaag
     8581 taagtttcct ataacttcag gaatcatatg tgctaaaggt aattctattg tttcaacctt
     8641 atcgacaaat atatttttta attcacctat tttcttagat tccactacgc ctaaagggag
     8701 acgcattaat ttttgagcta attttttacg aatttcagat aaaatttcat atgccgtaat
     8761 atgtgatagc atcgttgacg ctccaaaaca acacacttgt gaaatataag cgattaaagc
     8821 aataaagata taaaccataa tcgaattaat cgtatatgta ttgttaat
//
LOCUS       DAKTIO010000113         8756 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.113, whole genome shotgun sequence.
ACCESSION   DAKTIO010000113 DAKTIO010000000
VERSION     DAKTIO010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8756
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.113"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..303
                     /gene="deoC"
                     /locus_tag="O0293_001931"
     CDS             <1..303
                     /gene="deoC"
                     /locus_tag="O0293_001931"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCY0280812.1"
                     /translation="VKVIIETVLLDHDEIVKASELTKTAGADFVKTSTGFAGGGATAE
                     DVKLMKDTVGADVEVKASGGVRNLEDFNKMVEAGATRIGASAGVQIMQGLEADSDY"
     gene            331..1509
                     /gene="deoB"
                     /locus_tag="O0293_001932"
     CDS             331..1509
                     /gene="deoB"
                     /locus_tag="O0293_001932"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCY0280813.1"
                     /translation="MTRPFNRVHLIVMDSVGIGEAPDAADFKDEGSHTLRHTLEGFDQ
                     TLPNLEKLGLGNIDKLPVVNAVEQPEAYYTKLSEASVGKDTMTGHWEIMGLNIMQPFK
                     VYPNGFPEELIQQIEEMTGRKVVANKPASGTQIIDEWGEHQMKTGDLIVYTSADPVLQ
                     IAAHEDIIPLEELYDICEKVRELTKDPKYLIGRIIARPYVGEPGNFTRTSNRHDYALK
                     PFGKTVLDHLKDGGYDVIAIGKINDIYDGEGVTEAVRTKSNMDGMDQLMKIVKKDFTG
                     ISFLNLVDFDALYGHRRDKPGYAQAIKDFDDRLPELFSNLKEDDLVIITADHGNDPTA
                     PGTDHTREYIPVIMYSPKFKGGHALESDTTFSSIGATIADNFNVTLPEFGKSYLKELK
                     "
     gene            complement(1640..2455)
                     /gene="phnE"
                     /locus_tag="O0293_001933"
     CDS             complement(1640..2455)
                     /gene="phnE"
                     /locus_tag="O0293_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCY0280814.1"
                     /translation="MTQEIAKYNVHTKAHKRKLIKRWLIAIVVLAIIIWAFAGVPSLE
                     LKSKSLEILKSIFSGLFHPDISYIYIPDGEDLLRGLLETFAIAVVGTFIAAIICIPLA
                     FLGANNMVKLRPVSGVSKFILSVIRVFPEIVMALIFIKAVGPGSFSGVLALGIHSVGM
                     LGKLLAEDIEGLDFSAVESLKASGANKIKTLVFAVIPQIMPAFLSLILYRFELNLRSA
                     SILGLIGAGGIGTPLIFAIQTRSWDRVGIILIGLVLMVAIVDLISGSIRKRIV"
     gene            complement(2452..3252)
                     /gene="phnE"
                     /locus_tag="O0293_001934"
     CDS             complement(2452..3252)
                     /gene="phnE"
                     /locus_tag="O0293_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCY0280815.1"
                     /translation="MPLEIPTKYDSLLKKKVSLKTSFTFMLIIVLIIWSFIYTGFNFG
                     DLMIGIPQIGDLFKQMIPPDFEYLQQITTPMLDTIRMAIVSTVLGSIVSIPIALLCAS
                     NIVHQKWISIPSRFILNIVRTIPDLLLAAIFVAVFGIGQIPGILALFILTICIIGKLL
                     YESLETIDPGPMEAMTAVGANKIKWIVFGVVPQAISSFMSYVLYAFEVNIRASAVLGL
                     VGAGGIGLFYDQTLGLFQYPKTATIILFTLVIVVVIDYISTKVRAHLA"
     gene            complement(3254..4027)
                     /gene="phnC"
                     /locus_tag="O0293_001935"
     CDS             complement(3254..4027)
                     /gene="phnC"
                     /locus_tag="O0293_001935"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCY0280816.1"
                     /translation="MSQIEFKNVSKVYPNGHVGLKNINLNIEKGEFAVIVGLSGAGKS
                     TLLRSVNRLHDITSGEIFIQGKSITKAHGKALLEMRRNIGMIFQHFNLVKRSSVLRNV
                     LSGRVGYHPTWKMVLGLFPKEDKIKAMDALERVNILDKYNQRSDELSGGQQQRISIAR
                     ALCQESEIILADEPVASLDPLTTKQVMDDLRKINQELGITILINLHFVDLAKEYGTRI
                     IGLRDGEVVYDGPASEATDDVFSEIYGRTIKEDEKLGVN"
     gene            complement(4241..5197)
                     /locus_tag="O0293_001936"
     CDS             complement(4241..5197)
                     /locus_tag="O0293_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCY0280817.1"
                     /translation="MKNFKCLFVLMLAVIVFAAACGNSSSLDNQKNASNDSDSKSGGY
                     KPKELTVQFVPSQNAGTLEAKAKPLEKLLSKELGIPVKVSVSTNYNTIVEAMKSKKVD
                     VGFLPPTAYTLAHDQKAADLLLQAQRFGVKEDGSASKELVDSYKSEILVKKDSKIKSL
                     KDLKGKKIALQDVTSTAGYTFPLAMLKNEAGINATKDMKIVNVKGHDQAVISLLNGDV
                     DAAAVFNDARNTVKKDQPNVFKDTRILKLTQAIPNDTISVRPDMDKDFQEKLKKAFID
                     IAKSKEGHKIISEVYSHEGYTETKDSNFDIVREYEKLVKDMK"
     gene            5426..6970
                     /locus_tag="O0293_001937"
     CDS             5426..6970
                     /locus_tag="O0293_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCY0280818.1"
                     /translation="MKKIYKSLTVSAIVATVSLSALPQSLAITHESQPTKQQRTVLFD
                     RSHGQTAGAADWVSDGAFSDYADSIQKQGYDVKAIDGHSNITEASLKSSKIFVIPEAN
                     IPFKESEQAAIVKYVKQGGNVVFISDHYNADRNLNRIDSSEAMNGYRRGAYEDMSKGM
                     NAEEKSSTAMQGVKSSDWLSTNFGVRFRYNALGDLNTSNIVSSKESFGITEGVKSVSM
                     HAGSTLAITNPEKAKGIVYTPEQLPAKSKWSHAVDQGIYNGGGKAEGPYVAISKVGKG
                     KAAFIGDSSLVEDSSPKYVREDNGEKKKTYDGFKEQDNGKLLNNITAWMSKDNDGKSL
                     KASSLTLDTKTKLLDFERPERSTEPEKEPWSQPPSGYKWYDPTTFKAGSYGSEKGADP
                     QPNTPDDHTPPNQNEKVTFDIPQNVSVNEPFEMTIHLKGFEANQTLENLRVGIYKEGG
                     RQIGQFSSKDNDYNPPGYSTLPTVKADENGNVTIKVNAKVLESMEGSKIRLKLGDKTL
                     ITTDFK"
     gene            7021..8556
                     /locus_tag="O0293_001938"
     CDS             7021..8556
                     /locus_tag="O0293_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCY0280819.1"
                     /translation="MSNIAFYVVSDVHGYIFPTDFTSRNQYQPMGLLLANHVIEQDRR
                     QYDQSFKIDNGDFLQGSPFCNYLIAHSGSSQPLVDFYNRMAFDFGTLGNHEFNYGLPY
                     LKDTLRRLNYPVLCANIYENDSTLTDNGVKYFQVGDQTVGVIGLTTQFIPHWEQPEHI
                     QSLTFHSAFEILQQYLPEMKRHADIIVVCYHGGFEKDLESGTPTEVLTGENEGYAMLE
                     AFSKDIDIFITGHQHRQIAERFKQTAVIQPGTRGTTVGRVVLSTDEYENLSVESCELL
                     PVIDDSTFTIDEDDQHLRKQLEDWLDYEITTLPYDMTINHAFEARVAPHPFTNFMNYA
                     LLEKSDADVACTALFDSASGFKQVVTMRDVINNYPFPNTFKVLAVSGAKLKEAIERSA
                     EYFDVKNDEVSVSADFLEPKPQHFNYDIYGGVSYTIHVGRPKGQRVSNMMIQGHAVDL
                     KQTYTICVNNYRAVGGGQYDMYIDAPVVKDIQVEGAQLLIDFLSNNNLMRIPQVVDFK
                     VEK"
     gene            8631..8756
                     /locus_tag="O0293_001939"
                     /pseudo
     CDS             8631..8756
                     /locus_tag="O0293_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc peptidase"
BASE COUNT         2933 a         1427 c         1632 g         2764 t
ORIGIN      
        1 gtaaaagtga ttattgagac ggtattgttg gaccatgacg aaattgtaaa agcgagtgaa
       61 ttaacaaaaa cggctggtgc ggacttcgtt aaaacttcaa caggttttgc aggtggcggt
      121 gcgactgcag aagacgttaa attaatgaaa gatacagtag gtgctgatgt agaagtaaaa
      181 gcatcaggtg gcgtacgtaa tttagaagat ttcaataaaa tggttgaagc aggtgcgaca
      241 cgtattggtg cgagcgcagg tgttcaaatt atgcaaggtt tagaagcaga ttcagattac
      301 taatatatat aaattttggg agtgatagct atgacaagac catttaatcg tgtacattta
      361 atcgtaatgg attcagtagg tattggtgaa gcgccagacg cagctgattt taaagatgaa
      421 ggttcacata ctttaagaca taccttagaa ggtttcgatc aaactttacc aaaccttgaa
      481 aagttaggtc tagggaacat cgataaatta ccagtagtaa atgcagttga acaaccagaa
      541 gcatactata ctaaattgag tgaagcttca gttggtaaag atacaatgac tggtcactgg
      601 gaaattatgg gattaaatat tatgcaacct tttaaagtat accctaatgg attccctgaa
      661 gagttaattc aacaaattga agaaatgaca ggtcgtaaag ttgttgctaa caaaccggca
      721 tcgggtacgc aaattatcga tgagtggggc gagcaccaaa tgaaaactgg tgacttaatt
      781 gtttatacaa gtgcagaccc agtattgcaa attgctgcac atgaagacat tatcccatta
      841 gaagagttat atgatatttg tgaaaaggtt cgtgagttga caaaagaccc taaatattta
      901 attggtcgta ttatcgcacg tccatatgtt ggtgaaccag gaaactttac acgtacatct
      961 aatcgacatg actatgcgtt aaaacctttt ggtaaaactg tcttagatca tttgaaagac
     1021 ggtggttatg atgttattgc catcggtaaa attaatgaca tttatgatgg tgaaggtgta
     1081 acagaagcgg ttcgtacgaa gagtaacatg gacggtatgg atcaattgat gaaaattgtt
     1141 aagaaagatt tcacaggtat tagcttctta aacttagtag actttgatgc attatacggt
     1201 catcgtcgtg ataaaccagg ttatgcacaa gcaattaaag atttcgatga tcgcttgcca
     1261 gaactgttta gcaacttaaa agaagacgat ttagtaatta ttacagcaga ccatggtaat
     1321 gacccgacag cgccaggtac ggaccatacg agagaatata tcccagtaat tatgtacagt
     1381 ccgaaattta aaggtggtca tgcactagaa agtgatacta cattcagttc tatcggtgca
     1441 actatagcag ataatttcaa cgtaacatta ccagagttcg gtaaaagtta tttaaaggaa
     1501 ttgaaataga ataaatttag atattataaa aacagcagtg aagttaacta taacaatagt
     1561 tttcttcact gctgttttta ttataataga gaaacgtaag acggtaggac ttcttattta
     1621 ggagtatcct gatttaatgt taaacaatac gttttcggat tgaaccggaa attaaatcga
     1681 caattgcgac cattagtact aaaccgatta atataatacc tacacggtcc caagaacgtg
     1741 tttgaatggc aaatatgagt ggtgtcccga taccaccagc cccaattagc cccagtatag
     1801 aagctgaacg taagtttagt tcaaagcgat aaagtatgag tgatagaaag gcaggcataa
     1861 tttgtggtat gactgcaaat acgagtgttt taatcttatt cgcaccactg gcctttaatg
     1921 attctacagc actgaaatct agaccttcaa tatcttcagc taaaagtttc ccaagcatac
     1981 ctacggaatg gatacctaaa gctaatacac ctgaaaatga acctgggcca acagctttga
     2041 taaatataag tgccattaca atttctggga agacacgtat aacacttaaa ataaatttgc
     2101 taacacctga aactgggcgt agctttacca tattatttgc acctagaaat gctaatggaa
     2161 tacagataat tgcggcgatg aaagtaccta caacggctat cgcaaaggtt tcaagtaaac
     2221 cacgtaataa gtcttcgcca tctggtatat agatatagct gatatcagga tggaataatc
     2281 cgctgaatat ggattttaag atttctaatg atttactttt aagttctaaa cttggtacac
     2341 ctgcaaatgc ccagatgata atagctaaga cgacaattgc aataagccat cttttaatca
     2401 attttcgttt gtgtgctttt gtgtgaacat tatattttgc tatttcctgt gtcatgcgag
     2461 atgtgccctc actttcgtac tgatgtaatc aatgacgacg acgataacta aagtaaataa
     2521 aataatcgtt gctgtttttg gatattgaaa taaaccaagt gtttgatcat aaaacaatcc
     2581 aataccgcca gcgccgacta atccaagcac agctgaagca cgtatattta cttcaaatgc
     2641 atataatacg tatgacataa atgacgatat ggcttgtggt acaacaccga aaacaatcca
     2701 ttttatttta ttagcgccaa cagccgtcat tgcttccatt ggacctggat ctatcgtttc
     2761 caatgattca tataataatt ttccaataat acagatagtt aaaataaaca gtgctaatat
     2821 ccctggaatt tgaccgattc caaatacagc cacaaagatt gctgctaata acaaatctgg
     2881 aatagtacga actatattta aaataaagcg cgagggtatt gaaatccact tttgatgaac
     2941 gatattgcta gcacataata acgcaattgg tattgaaacg atgctaccta atactgtact
     3001 tacgatagcc attcgaatgg tatctaacat tggcgttgta atttgttgta aatactcgaa
     3061 atcaggtgga atcatttgtt tgaatagatc acctatttga ggtattccta tcattaaatc
     3121 tccaaaatta aatcccgtat aaatgaagct ccaaatgata agcacaatga ttaacatgaa
     3181 ggtaaaactc gtttttaaag aaaccttttt ctttaaaagg gagtcatact ttgtaggtat
     3241 ttctaaaggc atgttagttc actcctagct tttcatcttc tttaattgta cgtccatata
     3301 tttcactaaa tacgtcatct gttgcttcag atgcaggacc atcatagaca acttcaccat
     3361 cacgtaaacc aatgatgcgt gtgccatatt cttttgccaa gtcaacaaaa tgtaaattaa
     3421 ttaaaattgt gatgcctaat tcttggttga tttttcttaa atcatccata acctgtttcg
     3481 tagttaatgg gtctaatgaa gcaactggtt catctgcaag aataatttca gattcttggc
     3541 atagcgcacg tgcaatagat atacgttgtt gttggccacc tgataattca tcagagcgtt
     3601 gattatattt atctaagata ttgacgcgtt ctagtgcatc cattgcctta attttgtctt
     3661 cttttgggaa taaacctaat accattttcc aagtagggtg ataacctaca cgtccactta
     3721 gtacatttcg taatacactt gaccgtttaa ctaaattaaa atgttggaaa atcataccta
     3781 tatttcggcg catttctaat aatgctttac catgggcttt agtgattgat ttaccttgga
     3841 tgaaaatttc acctgacgtg atatcatgca aacgatttac agatcttaat aacgtggatt
     3901 tcccagcacc agatagtccg acaataactg caaattcacc tttttcaata tttaagttaa
     3961 tatttttcaa gcctacatga ccgttaggat agactttact gacgttttta aattcgattt
     4021 gactcatgtt tgacaccttt ctttaataaa gaaaagccaa ggtaaactga aatatcatag
     4081 acattgggtt tctgtgtaca aatggtttca aatgtacagc gacacctttc tatatctgtt
     4141 tcagtttagc gcctagcttt tcaatattaa tctagaatat atctattgaa cgaaagcttt
     4201 taataccaaa ttcgctaatg attcatttgt taaataatga ttatttcata tctttaacta
     4261 atttttcgta ctctcttaca atgtcgaaat ttgaatcttt cgtttctgtg tatccttcat
     4321 gtgaataaac ttcgctaata attttgtgac cttcttttga tttagcaatg tctataaaag
     4381 cttttttcaa tttttcttga aaatctttat ccatatctgg tcttacagaa attgtgtcat
     4441 tcggaatagc ttgtgttaat tttaaaattc gtgtgtcttt aaatacattt ggttggtctt
     4501 ttttcacagt attacgtgca tcgttaaata cagccgcagc atctacatct ccatttaata
     4561 atgagataac tgcttggtca tgacctttaa cattcacaat tttcatatct ttagttgcat
     4621 taatacctgc ttcgtttttt aacatcgcaa gtgggaatgt atatccagca gttgatgtta
     4681 catcttgtaa ggcaattttc ttacctttta aatctttcaa gcttttaatt tttgagtctt
     4741 ttttaacaag aatttctgat ttataactat ctacaagttc tttacttgct gaaccatctt
     4801 cttttacacc gaaacgttgt gcttgtaata ataaatcagc tgctttttga tcatgtgcta
     4861 atgtgtatgc cgttggtggt aagaaaccaa catcaacttt tttagacttc atagcttcaa
     4921 caattgtatt gtagttagtt gatacagaca ctttaactgg aatccctaat tctttagata
     4981 gtaatttttc taatggtttt gctttagctt ctaatgttcc agcattttgc gaaggtacaa
     5041 attgaacggt taattcttta ggtttgtatc ctcctgattt agaatccgaa tcattactag
     5101 cgttcttttg attatctaaa gaacttgagt ttccacatgc tgctgcaaaa acaatgactg
     5161 ctaacattaa tacaaataaa cacttaaaat ttttcatttg ataactgtcc ccttcacatt
     5221 cttaatcgtg taatatgtat gcaatgaaat tacacttata gcgtaccaca tttaaatgtt
     5281 aaaaagtatt ttataaaaat gaattttaat aactttcttt aagagtttga taagattttg
     5341 taaatttttc gttaaattgc tcatatgcaa cgaattttta tcctataatt caagaacaaa
     5401 tttattttaa aaggagcctc acaaaatgaa aaaaatatat aagtcattaa ctgtctctgc
     5461 aattgttgca acggtatcat taagtgcttt accgcaatct ttagctataa cgcatgaatc
     5521 gcaacctaca aagcaacagc gaacggtatt attcgatcgt tctcatggtc aaacagctgg
     5581 tgctgcagat tgggttagtg atggtgcatt ttcagattat gcggattcaa tacaaaaaca
     5641 aggttatgac gttaaagcta ttgatggtca ttcgaacata acagaagcaa gtttgaaaag
     5701 ttccaaaata tttgtaattc ctgaggctaa cattcctttc aaagaatcag aacaggcagc
     5761 aattgttaaa tatgtgaaac aaggtggcaa tgttgtcttt atttcagatc attacaatgc
     5821 tgaccgaaat ttaaatcgta ttgattcatc ggaggcaatg aatggttatc gacgtggagc
     5881 atatgaagat atgtcgaaag gtatgaatgc agaagaaaaa agttctactg caatgcaagg
     5941 tgtgaaaagt tcagattggt tatctacaaa ctttggcgta cgttttcgat ataatgcact
     6001 aggtgattta aatacgagca atattgtttc ttcaaaagaa agtttcggta ttactgaagg
     6061 tgtgaaatct gtctctatgc atgccggatc gacattagca attactaatc cagagaaagc
     6121 aaaaggtatt gtgtatacac cagaacaatt gccagcgaaa agtaaatggt cacatgctgt
     6181 agatcaaggt atttataatg ggggcggtaa agcagaaggc ccctatgtag caatttctaa
     6241 agttggaaaa ggtaaagcag catttatcgg tgattcatca cttgtggaag atagttcgcc
     6301 caaatatgta agagaagata atggagaaaa gaagaaaaca tatgatggtt ttaaagaaca
     6361 agacaacggt aagctattaa ataatataac ggcttggatg tctaaagata atgatgggaa
     6421 atcacttaag gcgagtagcc taacattaga tacaaagact aagttgcttg attttgaacg
     6481 accagagcgt tcaactgagc ctgaaaaaga gccatggtca caaccgccga gtggttataa
     6541 atggtatgat ccaacaacat ttaaagcagg tagttatggc agcgaaaaag gcgcagatcc
     6601 tcagccaaac acaccagatg atcatacacc accaaatcag aacgaaaaag taacatttga
     6661 tatcccgcaa aatgtttctg taaatgagcc atttgaaatg acaatacatt taaaaggatt
     6721 tgaagcaaat caaacacttg aaaatcttag agttggtatt tacaaagaag gcggacgtca
     6781 aatcggacaa ttttcaagta aagataacga ttataaccca ccaggttaca gtactttgcc
     6841 aacagttaaa gcagatgaaa acggaaatgt cacaattaag gtcaatgcta aagtacttga
     6901 aagtatggaa ggttcaaaga ttcgtttaaa actcggtgac aaaaccttga ttacaacaga
     6961 cttcaaataa atatataata gaaataagaa agatgtttgt gattgaagga gtgagtgagg
     7021 atgtcaaaca tagcatttta tgtcgtgagt gacgtacatg gttatatttt cccaacagat
     7081 tttacgagta gaaatcaata tcaacctatg ggattgttac tagcgaatca tgttatagaa
     7141 caagacagaa ggcagtatga ccaaagtttt aaaatagata atggtgattt tttgcaaggg
     7201 tcaccatttt gtaattactt aatcgcgcat agcggcagta gccagccttt agttgatttt
     7261 tataatcgaa tggcattcga ctttggtacg cttggtaatc atgaatttaa ttatggatta
     7321 ccatacttaa aagacacttt acgcagactc aattatccag ttttgtgcgc taatatatat
     7381 gaaaatgata gtacattgac tgataacggt gtgaagtatt ttcaggttgg agatcaaact
     7441 gttggtgtga taggtttaac gacacaattt attccccatt gggaacaacc agagcatatt
     7501 cagtcactta cgtttcatag tgcttttgaa atacttcaac aatacttacc tgaaatgaag
     7561 cgacatgcag atatcattgt ggtttgttac catggtggat ttgaaaagga tttagaaagt
     7621 ggtacgccga ccgaagtatt aacgggtgaa aatgaaggat atgccatgtt agaagcgttt
     7681 tctaaagata tagatatctt tattacgggt caccaacatc gacaaattgc tgaaaggttt
     7741 aagcaaacgg ctgtgattca acctggtacg agaggtacaa ctgtaggcag agtagtcttg
     7801 agtactgatg aatatgaaaa tctatccgtt gaatcatgtg aattacttcc tgttatagat
     7861 gattccacat ttactattga tgaagatgac caacatttac gaaagcagtt agaggactgg
     7921 ttagattacg aaattactac attgccatat gatatgacga ttaatcatgc atttgaggca
     7981 cgtgtggcac cgcatccttt tacaaatttt atgaattacg ctttattaga aaaaagtgac
     8041 gcagatgttg cctgtacagc tttgtttgat tctgctagtg gtttcaagca agtcgtgacg
     8101 atgcgagatg ttattaacaa ttacccattt ccaaatacat ttaaagtttt agctgtaagt
     8161 ggtgccaaac ttaaagaagc cattgaacga tcagcagaat attttgacgt gaaaaatgat
     8221 gaagttagtg tgagcgcaga cttccttgaa cccaaaccac aacactttaa ttatgatata
     8281 tatggtggcg taagttatac cattcatgtt ggaagaccaa agggacaacg tgtgagcaat
     8341 atgatgattc aaggtcacgc agttgattta aagcagacat atacaatttg tgtaaataat
     8401 tatcgtgcag taggcggtgg tcagtatgat atgtatatcg acgcgccagt tgtaaaagat
     8461 attcaagttg aaggcgcaca attacttatt gattttttat caaataataa tttgatgcgc
     8521 atcccgcaag ttgttgattt taaagttgaa aagtgacgga tatatgtaaa acttacttga
     8581 ttttaatcgg gtaagttttt gtttttgttt aaagcgagaa agtattatct ttagacgacg
     8641 tgattgaaat gtcacgtcct gtcaaaatga atagtttatt aaagttgtat tttgatagaa
     8701 atttactgac accacaaaaa ttattgaatt atttaaaagt tgatgaaaca tttttg
//
LOCUS       DAKTIO010000114         8754 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.114, whole genome shotgun sequence.
ACCESSION   DAKTIO010000114 DAKTIO010000000
VERSION     DAKTIO010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8754
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.114"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..316
                     /locus_tag="O0293_001940"
     CDS             <1..316
                     /locus_tag="O0293_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCY0280820.1"
                     /translation="KFSKIYDILTEDMIMPNMIIFLDADLDVLKSRIAKRNRSFEHQI
                     EDEYLLKLKKDYREYYESLQSNGSNVVLIDTTSIDFLKNEQDYEDILHIILPMIGDIT
                     NE"
     gene            309..971
                     /locus_tag="O0293_001941"
     CDS             309..971
                     /locus_tag="O0293_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCY0280821.1"
                     /translation="MNNYGIPQNAIITIAGTVGVGKSTLTQALADKLNFKTSFENVEH
                     NPYLDKFYSDFERWSFHLQIYFLAERFKEQKRMFEYGGGFVQDRSIYEDVDIFAKMHE
                     EEGTMSKEDFKTYSDLFNAMVMTPYFPKPDVMIYLECNYDEVIDRIIERGREMEINTD
                     PEYWKKLFKRYDDWINSFNACPVVRININEYDIHKDPESLNPMINKIARIIQTYRQVD
                     TR"
     gene            complement(1087..1770)
                     /locus_tag="O0293_001942"
     CDS             complement(1087..1770)
                     /locus_tag="O0293_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCY0280822.1"
                     /translation="MEWILFDKDGTLIEFDRSWEKIGVRFVQSLLETFPVHNKEAALR
                     QLGVIKESIDPKSVMGSGSLQQIIQAFNDVTGQDTTDWSKSTSQKLVDERIPEINWVE
                     GVKEALIDLKAKGYQLGIVTSDTKKGVEQFLAHTNATSLFDLIISTEADAYEKPNPKV
                     LSPLFEQYNVDPQKVAIVGDTANDMKTASNANLGMAIGVLTGIATKEELHEADIILNS
                     AADILEALN"
     gene            complement(2020..3096)
                     /locus_tag="O0293_001943"
     CDS             complement(2020..3096)
                     /locus_tag="O0293_001943"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HCY0280823.1"
                     /translation="MSQAVKVERRETLKQKPNTSQLGFGKYFTDYMLSYDYDADKGWH
                     DLKIVPYGPIEISPAAQGVHYGQSVFEGLKAYKRDGEVALFRPEENFKRLNNSLARLE
                     MPQVDEAELLEGLKQLVDIERDWIPEGEGQSLYIRPFVFATEGALGVGASHQYKLLII
                     LSPSGAYYGGETLKPTKIYVEDEYVRAVRGGVGFAKVAGNYAASLLAQTNANKLGYDQ
                     VLWLDGVEQKYIEEVGSMNIFFVENGKVITPELNGSILPGITRKSIIELAKNLGYEVE
                     ERRVSIDELFESYDKGELTEVFGSGTAAVISPVGTLRYEDREIVINNNETGEITQKLY
                     DVYTGIQNGTLEDKNGWRVVVPKY"
     gene            complement(3431..4396)
                     /locus_tag="O0293_001944"
     CDS             complement(3431..4396)
                     /locus_tag="O0293_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCY0280824.1"
                     /translation="MKKIMITGALGQIGTELVVKCREIYGTDNVLATDIREPEADSPV
                     QNGPFEILDVTDRDRMFELVRDFEADSLMHMAALLSATAEKNPILAWDLNMGGLMNAL
                     EAARTYNLHFFTPSSIGAFGDSTPKVNTPQVTIQQPTTMYGVNKVAGELLCQYYFKRF
                     GVDTRSVRFPGLISHVKEPGGGTTDYAVEIYFKAVREGHYTSFIDKGTYMDMMYMDDA
                     IEAIIKLMEADDAKLETRNGYNLSAMSFDPEMVKEAIQEYYPNFTLDYDVDPIRQGIA
                     NSWPDSIDTSCSRGEWGFDPKYDLASMTKLMLEAIEQKDTVKNNN"
     gene            complement(4610..6247)
                     /locus_tag="O0293_001945"
     CDS             complement(4610..6247)
                     /locus_tag="O0293_001945"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCY0280825.1"
                     /translation="MSYSIGIDYGTASGRVFLINTTNGQVVSKFVKPYTHGVIESELN
                     GLKIPHTYALQNSNDYLEIMEEGISYIVRESKIDPDNIVGIGIDFTSSTIIFTDENLN
                     PVHNLKQFKNNPHAYVKLWKHHGAYKEAEKLYQTAIENNNKWLGHYGYNVSSEWMIPK
                     IMEVMNRAPEIMEKTAYIMEAGDWIVNKLTNKNVRSNCGLGFKAFWEEETGFHYDLFD
                     KIDPKLSKVIQDKVSAPVVNIGEAVGKLDDKMAQKLGLSKETMVSPFIIDAHASLLGI
                     GSEKDKEMTMVMGTSTCHLMLNEKQHQVPGISGSVKGAIIPELFAYEAGQSAVGDLFE
                     YVAKQAPKSYVDEAENRNMTVFELMNEKIKHQMPGESGLIALDWHNGNRSVLSDSNLT
                     GCIFGLTLQTKHEDIYRAYLEATAFGTKMIMQQYQDWHMEVEKVFACGGIPKKNAVMM
                     DIYANVLNKKLIVMDSEYAPAIGAAILGAVSGGAHNSINDAVDAMKEPILYEINPEAE
                     KVQRYETLFKAYKALHDIHGYKKANIMKDIQSLRVEG"
     gene            complement(6407..7285)
                     /gene="hchA"
                     /locus_tag="O0293_001946"
     CDS             complement(6407..7285)
                     /gene="hchA"
                     /locus_tag="O0293_001946"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCY0280826.1"
                     /translation="MSQDVNELSKQPTPDKAEDNAFFPSPYSLSQYTAPKTDFDGVEH
                     KGAYKDGKWKVLMIAAEERYVLLENGKMFSTGNHPVEMLLPLHHLMEAGFDVDVATLS
                     GYPVKLELWAMPTEDEAVISTYNKLKEKLKQPKKLADVIKNELGPDSDYLSVFIPGGH
                     AAVVGISESEDVQQTLDWALDNDRFIVTLCHGPAALLSAGLNREKSPLEGYSVCVFPD
                     SLDEGANIEIGYLPGRLKWLVADLLTKQGLKVVNDDMTGRTLKDRKLLTGDSPLASNE
                     LGKLAVNEMLNAIQNK"
     gene            complement(7551..8738)
                     /locus_tag="O0293_001947"
     CDS             complement(7551..8738)
                     /locus_tag="O0293_001947"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCY0280827.1"
                     /translation="MVQSLHEFLEENINYLKENGLYNEIDTIEGANGPEIKINGKSYI
                     NLSSNNYLGLATNEDLKSAAKAAIDTHGVGAGAVRTINGTLDLHDELEETLAKFKGTE
                     AAIAYQSGFNCNMAAISAVMNKNDAILSDELNHASIIDGCRLSKAKIIRVNHSDMDDL
                     RAKAKEAVESGQYNKVMYITDGVFSMDGDVAKLPEIVEIAEEFGLLTYVDDAHGSGVM
                     GKGAGTVKHFGLQDKIDFQIGTLSKAIGVVGGYVAGTKELIDWLKAQSRPFLFSTSLA
                     PGDTKAITEAVKKLMDSTELHDKLWNNAQYLKNGLSKLGYDTGESETPITPVIIGDEK
                     TTQEFSKRLKDEGVYVKSIVFPTVPRGTGRVRNMPTAAHTKDMLDEAIAAYEKVGKEM
                     KLI"
BASE COUNT         2746 a         1663 c         1174 g         3171 t
ORIGIN      
        1 gaaattcagt aaaatttatg atattttaac tgaagatatg attatgccga atatgattat
       61 ctttttagat gcagaccttg atgtgttaaa atctagaatt gctaaacgta accgtagttt
      121 tgagcatcaa atagaagatg aatatctgtt aaagttaaaa aaagattatc gtgagtatta
      181 tgagtcctta caaagtaatg gttcaaatgt agttttaatc gatacaactt ctattgattt
      241 tcttaaaaat gaacaagatt acgaagatat attacatatt atattaccta tgataggaga
      301 tattaccaat gaataattac ggtattccac aaaatgccat tataaccatt gcaggtacag
      361 ttggtgttgg aaaatcaaca ctaacgcaag cacttgcaga taaattaaac tttaaaacgt
      421 cttttgaaaa tgtcgaacat aatccatatt tagataaatt ttacagcgat tttgaacgat
      481 ggagtttcca tttgcaaatt tacttcttag ctgaacgttt taaagaacaa aagcgtatgt
      541 ttgaatatgg tggtggcttt gtccaagatc gatcaattta tgaagatgtt gatatttttg
      601 caaaaatgca tgaagaagaa ggcacaatga gtaaagaaga tttcaaaaca tattcagact
      661 tatttaatgc catggtcatg acaccttatt ttcctaaacc tgatgtaatg atttatttag
      721 aatgtaacta tgatgaggtc attgatcgta ttattgaacg tggtcgcgaa atggaaatta
      781 atacagaccc tgaatactgg aaaaagctat ttaaacgcta tgacgattgg attaatagct
      841 ttaatgcatg tccagttgta cgtatcaata ttaatgaata tgatatccat aaggaccccg
      901 aatctttaaa tcctatgata aacaaaattg ctcgaattat tcaaacatat cgacaagtag
      961 atacacgata aaagactaaa gacatagcgt atatgtttat attcaatgta tattccatag
     1021 atattatcga ttattttatc aattctatcg aatacattaa ttcacatata cactatgtct
     1081 ttctttttaa tttaaagctt ctaaaatatc tgccgcacta tttaaaataa tatcagcttc
     1141 atgtaattct tcttttgttg caatacctgt taatacacct attgccatac ctaaatttgc
     1201 attacttgct gtcttcatat cattagcagt gtctcctact atagctactt tctgaggatc
     1261 tacattatat tgctcaaata aaggcgataa tactttagga tttggcttct cataggcatc
     1321 cgcttcggta gaaatgatca aatcgaacaa cgaggtagca ttggtatgtg ctaaaaattg
     1381 ttctacacct tttttagtat cactcgtaac aataccaagt tgatagcctt ttgctttcaa
     1441 atcgataagt gcttctttaa caccttctac ccaattaatt tcaggaatac gttcatctac
     1501 cagcttttga cttgttgact tggaccagtc ggttgtatct tgtcccgtca catcattaaa
     1561 tgcctggata atttgttgta aagatcctga acccatcact gattttggat caatagattc
     1621 tttaatgaca ccgagttgtc ttaaagcagc ttctttatta tgtactggga aagtctcaag
     1681 caatgattgt acaaatcgta cccctatttt ttcccaactt ctatcaaatt caattaacgt
     1741 accatcttta tcaaataata tccattccat tgatatcaat actcctattt atttatttcg
     1801 tattatgctg attctatgat attcgttatc ccctgaaaat gaactcgtag tattgttcta
     1861 tttaaatatt gaattaaata taataataag tgaaatcccc ttcaatactt aacaataaac
     1921 attgtaaact taatttatta ccatgcttcg cttcattgaa agggatttta gtcatgatta
     1981 acttttgcat attgttttca tgattatatt caatttttat taatattttg gtacaacgac
     2041 tctccaacca tttttatctt ctaaagtacc attttgaata ccagtataga cgtcgtataa
     2101 tttttgagta atttcaccag tctcattatt attaataacg atttcacgat cttcgtatct
     2161 caatgtaccc acaggtgaaa taactgctgc agtaccacta ccaaatactt ctgttaactc
     2221 acctttatca tatgattcga ataattcatc gattgaaacg cggcgctctt cgacttcata
     2281 tcctaagttt ttagctaatt cgataataga tttacgtgta ataccaggta aaatactgcc
     2341 attcaactct ggtgtaatta ctttgccatt ttcaacgaag aaaatgttca tgctaccaac
     2401 ttcttcgata tatttctgtt caacaccatc aagccataat acttggtcat aacctaattt
     2461 atttgcatta gtttgtgcta ataaacttgc cgcatagtta cctgcaactt ttgcaaagcc
     2521 tacaccgcca cgaacagcac gcacatattc atcttctaca tagattttag ttggttttaa
     2581 agtttcacca ccataatatg cacctgaagg agataaaata attaataatt tatactgatg
     2641 tgatgcacca acgccaagtg ccccttctgt tgcaaaaaca aatggacgaa tatataatga
     2701 ttgaccttcc ccttcaggaa tccaatctct ttcaatatca actaattgtt ttagcccctc
     2761 taacaattct gcttcgtcta cttgaggcat ttctaatcgt gctaacgagt tattaagacg
     2821 cttaaaattt tcttcaggac ggaaaagtgc aacttcccca tctcttttat atgcttttaa
     2881 tccttcgaat accgattgac cataatgaac accttgtgca gcaggtgaaa tttcaatagg
     2941 accataaggt actatcttca aatcatgcca tcctttatct gcatcataat cataactcaa
     3001 catataatca gtaaaatatt taccaaaacc tagttgagat gtatttggtt tttgttttaa
     3061 tgtttctcgt cgttcaactt taactgcttg tgacatggtg attgcctcct aataatattg
     3121 tataagaatt tgtttaactt aaattataac aatccatatt ttgctgttca acaaattttc
     3181 taaaaattca aaattaatta acagatttct agaaagacta tatcttttag tataaacgta
     3241 ttaatttcac agagacaagt aatctgtgtt ttactaatat actttacata caaaaaactc
     3301 tttactttaa aatgaactaa gctcgcgaat tcaataagta taatgaataa tattagaatt
     3361 catgcactag tttattaaaa taaagagtaa tttaaaatat cattccgtgt attaaagtga
     3421 atggaaatga ttagttatta tttttaacag tatctttttg ttcaatagct tctaacatta
     3481 atttagtcat gctcgctaaa tcatatttag gatcaaatcc ccattcgcca cgtgaacagc
     3541 ttgtatcaat agaatccggc caactattag cgataccttg tctaatagga tcaacatcgt
     3601 aatctaatgt aaaattggga tagtattctt gaattgcttc ttttaccatc tctggatcaa
     3661 aactcattgc gctcaaatta taaccatttc tagtttctaa tttagcgtcg tctgcttcca
     3721 taagtttaat aattgcttca attgcatcat ccatatacat catatccata tacgtgcctt
     3781 tatctatgaa gcttgtataa tgaccctctc ttactgcttt gaagtatatt tcaacagcat
     3841 agtctgtagt accgccacct ggctctttaa catgcgagat taaacctggg aatctaacac
     3901 ttcttgtatc tacaccaaaa cgtttgaaat agtattgaca caataattct ccagctactt
     3961 tatttacacc atacattgtc gtaggttgct gaatcgttac ttgtggcgta ttaactttag
     4021 gagttgagtc tccaaatgca ccaattgaac ttggtgtgaa aaagtgcaaa ttataagttc
     4081 ttgcagcttc taatgcattc attaatccac ccatatttaa atcccaagct agaattggat
     4141 ttttctcagc agttgctgat aataatgctg ccatatgcat tagactatcc gcttcaaagt
     4201 ccctaactaa ctcaaacata cggtcacgat ctgttacgtc taagatttca aatggtccat
     4261 tttgtacagg tgagtctgct tcaggttccc taatatctgt agcaagaaca ttatctgtcc
     4321 cataaatttc tctgcactta acaactaatt ctgtaccaat ttgtcctaat gcaccagtaa
     4381 tcataatttt tttcatagaa atatctcctt tgtctcttct atatagctat agtccatcac
     4441 aagcggacat aatattcatt ttcataataa ttataatata aaagcgcttt cttgtatata
     4501 tgacatgtac atgttgctga tatttctgta aatggaaatt ctagttgtat taattgattt
     4561 tagtaattta tagcgtttat tattgctaat tactgtcaaa ttaaattttt tatccctcaa
     4621 ctcttaaact ctggatatct ttcattatat tagctttttt ataaccatgg atatcatgta
     4681 aagccttata agctttaaat aatgtttcat acctttgtac tttttccgct tctggattaa
     4741 tttcgtataa aattggctct ttcatagcat caactgcgtc attaattgaa ttatgtgcgc
     4801 caccactgac tgcacctaat attgctgcgc ctattgctgg tgcatactca ctatccataa
     4861 caattagttt tttattcagt acattcgcat agatatccat cataacagca ttctttttag
     4921 gtataccgcc acatgcaaat accttttcta cttccatatg ccaatcttga tactgttgca
     4981 taatcatctt agtaccaaat gctgtagctt ctaaatatgc tctataaata tcctcatgct
     5041 tagtttgtaa agttaatcca aagatacaac ctgttaaatt gctatcactt aatacacttc
     5101 gatttccatt atgccaatca agagcaatga gcccactttc acctggcatt tgatgtttta
     5161 tcttttcatt cattaattca aatacagtca tatttctatt ttctgcttca tctacatatg
     5221 actttggtgc ttgcttagcg acatactcaa acaaatcacc tactgctgat tgccccgctt
     5281 cataagcaaa taattctgga ataattgctc cttttacaga acctgatata cctggcactt
     5341 gatgttgctt ttcatttaac ataagatggc atgtgcttgt tcccatcacc atagtcattt
     5401 ctttatcttt ttcagaccca atacctaata aactagcatg ggcatcaata ataaaaggac
     5461 ttaccatagt ttcttttgat aatcctaatt tctgtgccat tttatcatcc agtttcccta
     5521 ctgcttcacc aatattaaca accggtgcag atactttatc ttgaattact tttgataatt
     5581 tggggtctat tttatcaaat aaatcataat gaaaccctgt ttcttcttcc caaaatgctt
     5641 tgaaacctaa tccacaattc gagcgtacat ttttattagt taatttattt acaatccaat
     5701 cgcccgcttc cataatataa gccgtttttt ccataatttc tggtgctcga ttcatgacct
     5761 ccattatttt gggaatcatc cattcactac taacattata tccataatgg cctaaccact
     5821 tattattatt ttcaatagca gtttgatata atttctctgc ctctttatat gcaccatgat
     5881 gtttccaaag tttcacatac gcatgtggat tgtttttaaa ttgttttaag ttatgtaccg
     5941 ggttaaggtt ttcgtcagta aaaataatag tagatgaagt aaagtctata ccaataccta
     6001 ctatattgtc tggatctatt tttgattcac gtactatata tgatattcct tcttccataa
     6061 tttctaaata atcattacta ttttgaagtg catatgtatg tggtattttc aaaccattta
     6121 attcactctc aatgacacca tgtgtatatg gtttcacaaa ttttgatact acttgaccgt
     6181 tagttgtatt aattaaaaac acacggcctg aagcagttcc ataatcaatt ccaatgctat
     6241 aagacatcat gtttcctcca tatcgctagt tgcggttatt agttttgcaa gcgttttctt
     6301 aaatcatact cataataggt tctattttac aagtatcact taaattttgc caaaaaaaaa
     6361 gagccctcat acatttacat atgaggctcc tctaattaat atttaattat ttattttgta
     6421 ttgcatttaa catttcatta actgctaatt ttcctaactc atttgaagct aaaggactgt
     6481 cacctgttaa taatttacga tcttttaacg ttcttcctgt catatcgtcg ttaactactt
     6541 ttaatccttg tttagttaat aaatcagcaa ctaaccattt caagcgtcca ggtaaataac
     6601 ctatttcaat atttgcacct tcatctaatg agtcagggaa gacacaaaca gagtatcctt
     6661 ctaatggaga tttttctctg ttaagccctg ctgaaagtag tgctgctggt ccatgacata
     6721 atgtaactat aaagcggtca ttgtctaatg cccaatctaa tgtttgttga acgtcctcac
     6781 tttcagaaat accaacaact gcagcatgtc cgcctgggat aaagacagat aaatagtctg
     6841 aatcaggtcc taattcattt ttaatcacat ctgctaattt ttttggctgt tttaattttt
     6901 ctttcaattt attataagta cttataactg cctcgtcttc agttggcata gcccataatt
     6961 ctaatttaac tggataacca gataatgtcg caacatcaac gtcaaaacct gcttccatta
     7021 aatgatgtaa aggtaataac atttcaacag gatgattacc cgtagagaac atttttccat
     7081 tttccaataa tacatatcgc tcttcagcag caatcatcaa tactttccat ttaccatctt
     7141 tataggcacc tttgtgttca acaccatcaa aatctgtttt aggtgctgta tattgactaa
     7201 gggaatatgg tgatgggaaa aatgcgttat cttctgcttt atctggcgtt ggttgcttac
     7261 ttaattcatt tacatcttgt gacatagttt attcctactt tctctatata gttactacta
     7321 gtatagacat atactacaca ctataaacat gtattactat taaaatgctc attatcaaaa
     7381 ataatcaaaa tgaatttctt tgaaaaaaat acaactaaat tctacaagat gcttttaaaa
     7441 ttcaaacttc aattttgggg agcactaaac attgatatac gaacttactg ttaataaact
     7501 aaacattaaa aaaagcccac gacaatattt gccgtgggaa taaataaata ttaaatcaac
     7561 ttcatttctt ttcctacttt ttcataagcc gcaattgctt catctaacat gtcttttgta
     7621 tgtgcagctg taggcatatt tcttacacgt cctgtacctc ttggtactgt tgggaaaacg
     7681 atagatttca catagacacc ttcgtctttt aaacgcttac taaattcttg agttgttttt
     7741 tcatcaccaa taattactgg tgtaattgga gtttctgact cacctgtatc atatcctaat
     7801 tttgacaatc cattttttaa atattgtgca ttgttccata atttatcatg taattcagtt
     7861 gaatccatta actttttaac tgcttcagtt attgctttgg tatccccagg tgctaatgat
     7921 gtagagaata agaatggtcg tgattgtgct tttaaccaat ctattaactc ttttgtacct
     7981 gctacataac cgccaacgac accaattgct ttagaaagcg tacctatttg gaaatcgatt
     8041 ttatcttgta aaccaaaatg tttaaccgta ccagcgcctt tacccataac acctgaacca
     8101 tgagcgtcgt caacataagt taataaacca aattcttctg caatttctac aatttcaggt
     8161 aatttagcca catcaccatc catactaaaa acgccatcag tgatatacat cactttattg
     8221 tattgacctg attcaactgc ttcttttgct ttcgcacgta aatcatccat gtctgaatgg
     8281 ttaactcgaa taattttagc tttagataag cgacatccat caataattga tgcatgatta
     8341 agctcatctg ataaaatagc atcattttta ttcatgacag ctgaaatagc agccatatta
     8401 caattaaatc ctgattgata agctattgca gcttctgttc ctttaaattt tgctagtgtt
     8461 tcttctaatt cgtcgtgtaa atctaatgta ccattgattg tacgaacagc gcctgcacct
     8521 acaccatgtg tatcaatagc tgcttttgca gctgatttca aatcttcatt tgttgctagt
     8581 cctaaataat tatttgaaga taagttaatg tatgatttcc cattgatttt gatttctggt
     8641 ccgtttgcac cttcaattgt atctatttca ttatacaaac cattttcttt tagataattt
     8701 atattttcct ctaaaaactc atgtaatgat tgaaccacag tatataatcc ccct
//
LOCUS       DAKTIO010000115         8669 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.115, whole genome shotgun sequence.
ACCESSION   DAKTIO010000115 DAKTIO010000000
VERSION     DAKTIO010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8669
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.115"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..2312
                     /locus_tag="O0293_001948"
     CDS             <1..2312
                     /locus_tag="O0293_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HCY0280828.1"
                     /translation="IIYSTIRPPLSYSDNLNYILNKLSLHNDDFVKKIREIQKLILAY
                     EKTEIGFGEELYKDIIHHMKALFKCKNYLQIDTKIDMINNYLHQDIATNISEAAYLLW
                     LLSRNNIGFTDLKVLHNRFIEKYGFEQLVNIKDLISDITGFGTTIFQEEETDGNNIVM
                     LKQKFLHALRNNDEIVINEKDVESLINDNEINHYHAPMSADVYAELYLGRFYNQYNEL
                     IVISPLTASFNAGATFGRFHHLIDTETLAKLEHEKGHYYQKMICDDNVEMISINNIPK
                     YPRNHNVLTNHDSYEYSLNLGSSNSYSKYELTLDDIYVGATFNKLYLYSSQLNKRVLF
                     ESNNMYNFLKECNLYRLLREISMESVKCIEPMNDVSIDSFSYSPRIRYKNVILKPAYW
                     KINEMVLPLPKNEEWDQQFLKYQEQFNIPNIVNLVYGDNKLLLNLSLANHRYLLMKEY
                     KKHKRVRLVESFLPQSKNDHVYEIVTPIYKKSSYRGPEIEIPKYKNTDIEYDKDWFAL
                     HIHIDKPSQDTFIIDNLYPFVKHLKDKGDIDQYFLMRYIKQGDILKLRLFRNDENYAE
                     IYSILKNWLPHVRQTTEVSDYEFVSYEPEFFRYGGKNTINEIEAFFEYDTNLAVNIIE
                     NDFKFDRPYIVAISIMYLFEMFSISNEERMEIVNNYVPTSFKSKDIRPFKNELVTICN
                     PANNFEYMAKHYSGIYRILKDGNQILSKLNEGLKKTLTTKRSRIIGSLIHMRCNRIFG
                     IDKDQETFVLSIVKEIVKTQKHWCGDKND"
     gene            2305..3549
                     /locus_tag="O0293_001949"
     CDS             2305..3549
                     /locus_tag="O0293_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HCY0280829.1"
                     /translation="MIDMNLILKKKFQEISEVDDFISKASTETDYFEPSTLSHGIPGI
                     ILFLDAYQKVFDINTEQIVHKYIMKLAPYLQSGQYNNSLFGGLSGIAFSMDLASQNGK
                     NYQNILNNIDGHIVNEIENKFDQILQEPLNPLNYDTVSGLAGIGRYLLNRVDENEFNV
                     KALKSILVYFKDIQYSKNSWVVPQESQFLESDKNYFTEGNINLGLAHGVLGPMSLFAL
                     CVIKGITIENHQHILKDMYKFIMDEKFCNHERWLQRYDLISERNHFNFIRNGWCYGNT
                     GVMTTLFLIGQALQDDEIIKMSKKVMLQVVNDKDENLISPTICHGLSSQILMLTIMNL
                     NFELNEVSDYITVLINKLISHYKEDYLVNFIDINENKQDVFKSRKVGLLEGELGVYLT
                     LMTLKNTKILNEKNWTNAFLIS"
     gene            3565..4083
                     /locus_tag="O0293_001950"
     CDS             3565..4083
                     /locus_tag="O0293_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HCY0280830.1"
                     /translation="MGENVLICLCGSVNSINISHYIIELKSKFDEVNVIASTNGRKFI
                     NGEILKQFCDNYYDEFEDPFLNHVDIANKHDKIIILPATSNTINKIANGICDNLLLTI
                     CHTAFEKLSIFPNMNLRMWENPVTQNNIRLLKDYGVSIYPANISESYELASKTFKKNV
                     VAPEPYKVLEFI"
     gene            4093..5466
                     /locus_tag="O0293_001951"
     CDS             4093..5466
                     /locus_tag="O0293_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HCY0280831.1"
                     /translation="MKIIKRAIISLIILSLLISITMSNASASEELYYSVEYKNTATFN
                     KLVKKKSLNVVYNIPELHVAQIKMTKMHANALANYKNDIKYINATCSTCITSEKTIDR
                     TSNESLFSRQWDMNKITNNGASYDDLPKHANTKIAIIDTGVMKNHDDLKNNFSTDSKN
                     LVPLNGFRGTEPEETGDVHDVNDRKGHGTMVSGQTSANGKLIGVAPNNKFTMYRVFGS
                     KKTELLWVSKAIVQAANDGNQVINISVGSYIILDKNDHQTFRKDEKVEYDALQKAINY
                     AKKKKSIVVAAAGNDGIDVNDKQKLKLQREYQGNGEVKDVPASMDNVVTVGSTDQKSN
                     LSEFSNFGMNYTDIAAPGGSFAYLNQFGVDKWMNEGYMHKENILTTANNGRYIYQAGT
                     SLATPKVSGALALIIDKYHLEKHPDKAIELLYQHGTSKNNKPFSRYGHGELDVYKALN
                     VANQKAS"
     gene            5489..6181
                     /locus_tag="O0293_001952"
     CDS             5489..6181
                     /locus_tag="O0293_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding subunit"
                     /protein_id="HCY0280832.1"
                     /translation="MAKLVTENISKRFKNQDVLKHINITLENNEVYGLLGINGAGKTT
                     LMKIICGILQQDSGEIKLDNRPMTRNDLHKVGSLIETPATYNHLSAQDNLKIVCLNES
                     VDFSEINSVLSLVNLNVDKKKKVKDFSLGMKQRLGIAMALIKKPEILVLDEPSNGLDP
                     YGIQELRELLKLLTEQGTSIIISSHILSEIQVLADHIGIIHEGELKYQQRNNKDENLE
                     EIFFKITKGDYK"
     gene            6178..6939
                     /locus_tag="O0293_001953"
     CDS             6178..6939
                     /locus_tag="O0293_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HCY0280833.1"
                     /translation="MIHLKIEGIKFKNSFSMYVLLISPLVFLCFAIFTVLFAKSNTGT
                     ANSVSPYITLLFNIWPIAFIPIVLCMACNSLFKIEMRNKSFNYYLSNNWSITKEIRAK
                     IFILSIAFLVHCFLVFIIAYIGDLIINPHPVNAMLLLVTILLMYVVSLPLIPLNFLLT
                     RYFGVFVSILINLVLSVICVLFLTLKSLFWVLPWGIMQRIPLITLGILPNGLVVNHNS
                     KYFNDLNALYISIIVSIIIFAIVTFLNNKKSWRLK"
     gene            6936..7634
                     /locus_tag="O0293_001954"
     CDS             6936..7634
                     /locus_tag="O0293_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsaG protein"
                     /protein_id="HCY0280834.1"
                     /translation="MIINELKSCKLKFSKQALTFVPIIVTILFILFINWYLNVNLWNG
                     RQISLFTASFNAITSLLISINVYQVINFEENIGHFNHILGKANRLNWLNASMIFTYTI
                     TAICILLASINLLWHSHDMKITLMFIGVSLFFNVIILLLLFIFSIFIKDVMAIVVGVL
                     MFIFNVYFGLEVLGDHSWFYLPITYATRYVYMFIEGSIPVTLTLAIYIIITLLLAVLL
                     FKGFNKWSGRTIKD"
     gene            complement(8108..>8669)
                     /locus_tag="O0293_001955"
     CDS             complement(8108..>8669)
                     /locus_tag="O0293_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HCY0280835.1"
                     /translation="TKFLGKTLVASALVLTTLGTGLHSSYLGLDTNKVVKTAKAEEKM
                     TDGQLWKKVKDSLHDSDIILSNEYETINVTYLLSNGYSSSVSAPGNDDGGHLTQSIDF
                     KGLKQIDLTKENVYDDFNKKLDAKNTWNSLTEKLKGLGLLQNGQKVSIYSSDSSSPVS
                     GKVGEGVTSGGENTLTKRFINKITID"
BASE COUNT         3173 a         1096 c         1455 g         2945 t
ORIGIN      
        1 aaattatata ttcaacaatc agaccaccgt tatcatatag cgataattta aattatattt
       61 taaataaact ttctttacat aatgatgatt ttgtgaaaaa aattagagaa atccagaaat
      121 tgatattagc ttatgaaaaa actgaaattg gatttggcga ggaattatat aaagacatta
      181 ttcatcatat gaaggcacta ttcaaatgta aaaactatct tcaaattgat accaaaattg
      241 acatgattaa caattattta catcaagata tagcaacaaa tatatctgaa gcagcgtact
      301 tattgtggct tttatcaaga aataatattg gattcacaga tttgaaagtt cttcacaacc
      361 gttttattga aaagtatgga tttgaacaac tcgtgaatat taaagatttg atttcagata
      421 taacaggttt cgggacaaca atttttcaag aagaagaaac cgatggaaat aatattgtaa
      481 tgcttaagca aaaattttta catgccttga gaaataacga tgagatagtc ataaatgaaa
      541 aagatgtaga gtcattgatt aacgataatg aaatcaatca ttatcatgcg ccaatgagtg
      601 cagatgttta tgctgaactt tatttaggac gtttttacaa tcagtacaat gagctcattg
      661 ttattagtcc tttaacggcg tcttttaatg ctggcgctac atttgggcga tttcatcatt
      721 taattgatac tgaaacttta gcaaaattag aacatgaaaa aggacattat tatcagaaga
      781 tgatatgtga tgacaatgta gaaatgattt ctataaataa cataccgaaa tatccgagaa
      841 atcataatgt attaactaat catgactcat acgaatattc attgaattta ggaagtagta
      901 atagttattc aaagtatgag cttaccttag atgatattta tgttggtgct acctttaaca
      961 aattatattt atattctagc caactaaata aaagggtact atttgaatca aacaatatgt
     1021 ataacttttt aaaagaatgt aatttatatc gattgttacg agaaatttca atggaatctg
     1081 tgaaatgcat tgaacctatg aatgatgtga gtattgattc atttagttat tcaccacgaa
     1141 tcagatataa aaatgtcatt cttaaaccag catattggaa aattaacgag atggtattac
     1201 ctttacctaa aaatgaagaa tgggatcaac aatttttgaa atatcaagaa caattcaata
     1261 tacctaatat cgtgaattta gtttatggag ataacaagct tctactaaat ttatcattag
     1321 ctaatcatag atatttattg atgaaagaat ataagaagca taaacgtgta cgtttagtag
     1381 aatcattttt acctcaatca aagaatgatc atgtgtatga aattgttaca ccaatttata
     1441 aaaaatcatc ttatcgagga cccgaaatcg aaattccaaa atataaaaat acagatattg
     1501 aatatgacaa agactggttt gcattacata ttcatatcga taagccatcg caagatacat
     1561 ttatcatcga caatttgtat ccatttgtaa aacatcttaa agataaggga gatatagatc
     1621 aatatttttt aatgcgttat atcaaacaag gtgacatttt aaagttgagg ttattcagga
     1681 atgacgaaaa ttacgcagaa atatacagca tcttgaagaa ttggttacca catgttcgac
     1741 aaacgacaga agtttcagat tatgaatttg tatcatacga gccagaattt tttagatatg
     1801 gtggaaagaa cacgattaat gaaatagaag ccttctttga atatgacacg aatttagctg
     1861 tcaatattat tgaaaatgat tttaagtttg atcgaccata tattgttgct atttcaataa
     1921 tgtatttatt tgagatgttt tcaatttcta atgaagagcg tatggaaatt gttaataact
     1981 atgtacctac atcatttaaa agtaaagata ttagaccttt taaaaatgaa cttgtaacta
     2041 tatgtaatcc tgctaataat tttgaatata tggctaaaca ctatagtggt atttatcgaa
     2101 ttttaaaaga tggcaaccaa attttgagca aattaaatga agggcttaaa aaaactttaa
     2161 ctactaaaag gtcaaggatt attggcagct taattcatat gcgctgcaat cgaatatttg
     2221 gcattgataa ggatcaagaa acatttgttt tatctattgt aaaagaaatt gttaaaactc
     2281 aaaagcattg gtgtggtgac aaaaatgatt gatatgaatc taatattgaa gaagaagttt
     2341 caagagattt cagaagtaga tgatttcatt tcaaaagcat ctactgaaac cgattatttt
     2401 gaaccttcaa ctttatcaca tggtatacca ggtatcattt tgtttttaga tgcttatcaa
     2461 aaagttttcg atattaatac tgagcaaatt gtgcataagt acatcatgaa attagcacca
     2521 tatttacaga gtggtcaata taacaattct ttgtttggtg gactttcagg tattgcattt
     2581 tcaatggatt tagcatcgca aaatggcaaa aattatcaaa atattttaaa taatattgat
     2641 ggtcacattg taaatgaaat agaaaataaa tttgatcaaa tattacaaga accattaaat
     2701 ccattaaatt atgatactgt cagtggatta gctgggatag ggagatattt gctaaataga
     2761 gtagatgaga atgaatttaa tgttaaagca ttaaaaagca tattagtata ctttaaagat
     2821 attcaatatt ctaaaaatag ctgggtagtc ccacaagaaa gtcaattttt agagtctgat
     2881 aaaaattatt ttactgaagg taatatcaat cttggccttg cacatggagt gctaggaccg
     2941 atgtctttat ttgcattatg cgtgattaaa ggaattacga ttgaaaatca tcagcacata
     3001 ttaaaagaca tgtacaaatt tatcatggac gaaaaatttt gtaaccacga aagatggttg
     3061 cagcgttacg atttaatttc tgaacgtaat catttcaatt ttattcggaa tggttggtgt
     3121 tatggcaata cgggtgtaat gacgacgttg tttttaatcg gccaagcatt acaagatgat
     3181 gaaataatta aaatgtctaa aaaagtgatg ctacaagtag taaatgataa agatgaaaat
     3241 ttaataagtc caactatttg tcatggattg tcatcacaaa tattaatgtt aacaattatg
     3301 aatttgaatt ttgaattaaa tgaagtgtct gattatatca ctgtattaat aaataaactg
     3361 atttctcatt ataaggaaga ttatctggtg aattttatag acattaatga aaataagcaa
     3421 gatgtattta aaagtaggaa agttggcctt ttagaaggtg aattaggggt ctatctaaca
     3481 ttgatgacac tgaaaaatac aaaaattctc aatgaaaaaa attggaccaa tgcgttttta
     3541 attagctgaa tggaggcgta tatcatggga gaaaatgtat tgatttgttt gtgtggctca
     3601 gttaatagta tcaatattag ccactatatt attgaattaa aatcaaaatt tgatgaagta
     3661 aatgtaatcg cgtcaacaaa cggtagaaag tttattaatg gtgaaatatt aaagcaattt
     3721 tgcgataact attatgacga atttgaagac ccttttttaa atcatgtaga tatagcaaat
     3781 aaacatgaca agattattat tttacctgcg acttctaata cgattaataa aattgcaaat
     3841 ggtatatgtg acaatttatt attaactatt tgtcatacag cttttgaaaa actttctata
     3901 tttccaaata tgaatttacg aatgtgggaa aatccagtta ctcaaaataa tattcgatta
     3961 ttaaaagatt atggtgtatc aatatatcca gcaaatattt cagaaagtta tgaattagcg
     4021 tcaaaaacat ttaaaaagaa tgttgtcgca cctgaaccat ataaagttct ggaattcatt
     4081 tgagattaga atatgaaaat cataaagcgc gctattatta gtttaataat attgagtctt
     4141 ttaatatcta ttacaatgag taatgcatca gcgtcagaag aactatatta cagtgttgaa
     4201 tacaaaaata cagcaacatt taacaagtta gttaaaaaga agtccttaaa cgttgtctat
     4261 aatataccgg aattacatgt ggcacagatt aaaatgacga aaatgcatgc taatgcttta
     4321 gcaaactata aaaatgatat taaatatatc aatgccacat gttcaacttg tattactagc
     4381 gagaaaacaa tagacagaac atcaaatgag tcattatttt caagacaatg ggatatgaat
     4441 aaaataacca ataatggtgc atcgtatgat gatttgccaa aacatgctaa caccaaaata
     4501 gcaatcatag atacaggtgt gatgaaaaac catgacgatt tgaaaaataa tttctcgact
     4561 gattctaaaa atttagtacc tttaaacggt tttagaggta ctgaaccgga ggaaacaggt
     4621 gatgttcacg atgtcaatga taggaaagga catggcacga tggtgtcggg tcaaacgagt
     4681 gctaatggta agttaatagg tgttgcaccg aataacaaat ttacaatgta tcgcgtgttt
     4741 ggtagtaaaa aaacagaact gctttgggta tcaaaagcga ttgttcaagc tgcaaatgat
     4801 ggaaatcaag tcattaatat tagtgttggt agttatatta ttttggacaa aaatgaccat
     4861 caaacattta gaaaagatga aaaagtagaa tacgatgcgt tacagaaagc aatcaattac
     4921 gccaagaaga aaaaatctat cgttgttgct gcagctggta atgatggtat tgatgtcaat
     4981 gacaaacaga aactaaaatt acagcgtgaa tatcaaggta atggcgaagt gaaagatgtt
     5041 cctgcatcta tggacaatgt cgttacagta ggatctacag atcaaaagag taatctatct
     5101 gagttttcca attttggtat gaattataca gatattgctg cgcccggagg atcatttgct
     5161 tatttaaatc aattcggtgt ggataaatgg atgaatgaag ggtatatgca taaggagaac
     5221 attttaacta ctgccaataa cggaagatat atttatcaag ctggaacttc attagccaca
     5281 cctaaagttt cgggagcact agctttaatc attgataaat atcatcttga aaaacatcca
     5341 gataaagcga ttgaattgtt atatcagcat gggacatcta agaataataa accatttagt
     5401 agatatgggc atggtgagct tgatgtgtat aaagcattaa atgtagcaaa tcaaaaagca
     5461 agttaataaa tcaaaggagt ttttgattat ggcaaaatta gttactgaaa acatttcgaa
     5521 gcggtttaaa aatcaagatg tattaaagca tattaatatc actttagaaa ataacgaagt
     5581 ttatggatta cttggtatta atggagccgg taaaacgaca cttatgaaaa ttatatgtgg
     5641 catacttcaa caagattcag gggaaattaa attagataat agaccaatga cacgaaatga
     5701 tttgcacaaa gttggttcgc ttattgaaac acctgcgaca tataatcatt taagtgcaca
     5761 agataatttg aaaattgtgt gtttaaatga aagcgttgat ttcagcgaaa ttaatagtgt
     5821 tttaagctta gtcaatttaa atgtcgataa aaagaaaaag gttaaggact tttctttagg
     5881 tatgaaacaa agacttggaa ttgcaatggc attaattaaa aagccagaaa ttttagtatt
     5941 agacgaacca tctaatggtt tagacccata tggaatccaa gaacttagag aacttctaaa
     6001 attattaaca gaacaaggta ctagtattat tatttcaagt cacattttat ctgaaatcca
     6061 agttttagca gatcatatcg gtattattca tgagggtgag ctaaaatatc agcaaagaaa
     6121 taacaaagat gaaaacttag aagagatatt cttcaaaata acgaaaggtg attacaaatg
     6181 atacatttaa agatagaagg tatcaaattt aaaaattctt tcagtatgta tgttttatta
     6241 ataagtccgc tggtatttct ttgttttgct attttcacag tcttattcgc caaaagtaat
     6301 acgggaacag cgaatagtgt gtcaccatat ataactttac tatttaatat ttggccaatt
     6361 gctttcatcc cgattgtatt atgtatggct tgtaattcgt tatttaaaat tgaaatgaga
     6421 aataaatcat ttaattatta cttaagtaat aattggtcga ttacaaaaga aataagagca
     6481 aagattttca ttttatcaat agcatttttg gtacattgct ttttagtatt tattattgct
     6541 tatataggtg acctaattat taatccacat ccggttaatg ctatgttgtt attggtgaca
     6601 atattgttga tgtatgtagt atctctacca ttgataccgc tcaacttttt attaactcga
     6661 tactttggtg tgttcgtatc aatattaata aacttagtat tatcagtcat ttgtgtcttg
     6721 tttttaacat tgaagagttt attttgggtg ttgccgtggg ggataatgca gagaatcccg
     6781 cttattacgc ttggtatact acctaatggt ttagttgtaa accataattc aaaatacttt
     6841 aatgatctca atgccttata tatttcgatt attgttagca tcattatttt cgcgatagta
     6901 acatttttaa ataataagaa aagttggcga ttaaaatgat aattaacgaa ttaaagtcat
     6961 gtaagttgaa attttctaag caagcgctca catttgtacc cattattgta accatattgt
     7021 ttatattatt tataaattgg tatttaaacg taaatttatg gaatggtcga caaataagtt
     7081 tgtttacagc gagttttaat gcaattacat cgctattaat ttctataaac gtctatcaag
     7141 ttatcaattt tgaagaaaat attggtcact ttaatcatat tttaggaaaa gctaataggc
     7201 taaattggtt aaatgcatca atgattttta cttatactat tacagccata tgtattctat
     7261 tagcatcaat taatttattg tggcattcac atgatatgaa aataacactt atgtttatag
     7321 gcgtatcatt gtttttcaat gtaattatat tactgctact ttttattttt agtattttca
     7381 ttaaagatgt aatggctatt gttgtcggag ttttaatgtt tatttttaac gtttattttg
     7441 gattagaagt gcttggagat cattcgtggt tctatttacc aatcacatat gctacacgtt
     7501 acgtttatat gtttatcgaa gggagtatac cagttacatt aacattggca atctatatta
     7561 ttatcacgtt gctattagca gttttactct ttaaaggatt taataaatgg agcggtagaa
     7621 caattaaaga ttaaagattg gggctctaac ggtaaaagaa atttagttgc gttgaaaagg
     7681 gaggaaagag aacagttaaa tatgaataca aaataatttt gttttcggac agcaggggta
     7741 ttagacgcga ttgacaatgt ctgtttaatt aaacgtaatg tttatttaag cgatgaatat
     7801 taggtgaaaa gtttttgaat ttgaatgtaa ttgaggttta ttgattagac attttattga
     7861 attgcgtgtt attatataaa tgtaaaaata agacgacatg cgcgaacatg tcgtctaagc
     7921 aagccaaacg ctggcttcta aaatattttt ttaaccattc caacttgcca aagttaatgc
     7981 ggaatggttt ttttattttc cgctaattga aataaaaatg acgttttaat atattatggg
     8041 ctaggtggtt tgtaagaaag ggttagttat taatgtttta tgaattaagg aaatttgagt
     8101 ttaaggttta atcaattgtg attttgttga tgaagcgttt agttagagta ttttcgccac
     8161 cactagttac tccttctccc actttacccg agactggaga agagctatct gaagaataaa
     8221 tagatacttt ttggccattt tgtagtaaac caagaccttt taacttctcg gttagagaat
     8281 tccatgtatt tttagcatct agctttttgt taaagtcatc gtagacattt tccttagtta
     8341 aatcaatttg ttttaatcct ttaaagtcta tagattgtgt tagatggcct ccatcatcat
     8401 ttccaggagc agaaacgctg ctagaatatc cattgcttaa taaataagta acgttgattg
     8461 tttcgtactc gttacttaaa ataatatcag aatcatgtaa tgaatcttta acttttttcc
     8521 ataattgacc atctgtcatt ttttcttctg ctttagccgt tttaacaact ttatttgtat
     8581 ctaatcctaa gtatgaagaa tgtaagcctg ttcctaatgt tgttaatact aaagcacttg
     8641 ctactaatgt tttacctaaa aattttgta
//
LOCUS       DAKTIO010000116         8621 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.116, whole genome shotgun sequence.
ACCESSION   DAKTIO010000116 DAKTIO010000000
VERSION     DAKTIO010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8621
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.116"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..475
                     /locus_tag="O0293_001956"
     CDS             <1..475
                     /locus_tag="O0293_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0280836.1"
                     /translation="KLSSVQFGFSDMLYGIGGLCAGLISAILSKKISTKVLIFLLYFI
                     LVINSALFIWINSAFYLFIGSFILGYSISSIRIYMNTAIMNTVSDKYVGRSFTIWTSI
                     SLLLQSLIAPFLGRWINEINDKFGFYIILILSLLIFVTLLLVNKTGKIKYAHKEE"
     gene            complement(547..843)
                     /locus_tag="O0293_001957"
     CDS             complement(547..843)
                     /locus_tag="O0293_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HCY0280837.1"
                     /translation="MATKKDVHDLFLNHVNSNAVKRRKMMGEYIIYYDGVVIGGLYDN
                     RLLVKATKSAQQKLQDNTLVSPYPGSKEMILILDFTEATNLTDLFKTIKNDLKK"
     gene            complement(892..1095)
                     /locus_tag="O0293_001958"
     CDS             complement(892..1095)
                     /locus_tag="O0293_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280838.1"
                     /translation="MEGLKHSLKSLGWWDLFFAIPIFLLFAYLPNYNFITIFLNIVII
                     IFFSIGLILTTHIIIDKIKSNTK"
     gene            1404..2390
                     /locus_tag="O0293_001959"
     CDS             1404..2390
                     /locus_tag="O0293_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCY0280839.1"
                     /translation="MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEF
                     TNTESFCHPEGIHSVRGRLTFSEDEQPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLN
                     MGCPVANVAKKGKGSGLILRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKH
                     VFEQDIANLSIHLRTRKEMSKVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGL
                     ELAEKYGIDGVMIGRGIFHNPFAFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQF
                     KSLRRFFKIYVRGIRGASELRHQLMNTQSIAEARALLDEFEAQMDEDVKIEL"
     gene            complement(3057..4250)
                     /gene="sqr"
                     /locus_tag="O0293_001960"
                     /pseudo
     CDS             complement(3057..4250)
                     /gene="sqr"
                     /locus_tag="O0293_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498643.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II sulfide:quinone oxidoreductase Sqr"
     gene            complement(4269..5603)
                     /gene="cstB"
                     /locus_tag="O0293_001961"
     CDS             complement(4269..5603)
                     /gene="cstB"
                     /locus_tag="O0293_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
                     /protein_id="HCY0280840.1"
                     /translation="MFFKQFYDNHLSQASYLVGCQRTGEAIIIDPVRDLSKYIEVADS
                     EGLTITQATETHIHADFASGIRDVAKRLNANIYVSGEGEDALGYKNMPSKTQFVKHGD
                     IIQVGNVKLEVLHTPGHTPESISFLLTDLGGGSSVPMGLFSGDFIFVGDIGRPDLLEK
                     SVQIKGSTEISAKQMYESVQNIKNLPDYVQIWPGHGAGSPCGKALGAIPISTIGYEKI
                     NNWAFNEIDETKFIESLTSNQPAPPHHFAQMKQVNQFGMNLYQSYDVYPSLDNKRVAF
                     DLRSKEAFHGGHTKGTINIPYNKNFINQIGWYLDFEKDIDLIGDKSTVEKAKHTLQLI
                     GFDKVAGYRLPKSGISTQSVHSADMTGKEEHVLDVRNDEEWNNGHLDQAVNIPHGKLL
                     NENIPFNKEDKIYVHCQSGVRSSIAVGILESKGFENVVNIREGYQDFPESLK"
     gene            complement(5634..6701)
                     /gene="cstA"
                     /locus_tag="O0293_001962"
     CDS             complement(5634..6701)
                     /gene="cstA"
                     /locus_tag="O0293_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide response sulfurtransferase CstA"
                     /protein_id="HCY0280841.1"
                     /translation="MKQYGEKFIDEFSKAELEKLAKQGQLIDVRTEEEYALGHINGSI
                     LHPVDEIESFNKEKNKTYYVICRSGNRSANASKYLAKQGYNVINLDGGYKAYEEENDS
                     YDTQEEYKSIEIKADRKQFNYRGLQCPGPIVKISQEMKNIEVGDQIEVKVTDPGFPSD
                     IKSWVKQTRHTLVKLDENNNGINAIIQKEKAKDLDINYSAKGTTIVLFSGELDKAVAA
                     LIIANGARAAGKDVTIFFTFWGLNALKKVQTVNVKKQGIAKMFDLMLPKKNIRMPLSK
                     MNMFGLGNMMMRYVMKKKNVDSLPTLINQAIEQNIKLIACTMSMDVMGIQKEELRDEV
                     EYGGVGTYIGATENANHNLFI"
     gene            6838..7098
                     /gene="cstR"
                     /locus_tag="O0293_001963"
     CDS             6838..7098
                     /gene="cstR"
                     /locus_tag="O0293_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide-sensing transcriptional repressor
                     CstR"
                     /protein_id="HCY0280842.1"
                     /translation="MNYDKKMINRINRIQGQLNGIIKMMEEGKDCKDVITQISASKSS
                     LQRLMGIIISENLIECVKAAADDEESSQELINEAVNLLVKSK"
     gene            7098..7853
                     /locus_tag="O0293_001964"
     CDS             7098..7853
                     /locus_tag="O0293_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCY0280843.1"
                     /translation="MDIVNIVIMLLIGVFGGFISGLVGVGGAIIIYPAILLLPPLFGA
                     PAYSAYIASGLTSSQVFFSTLSGSLKARKKTEFSPQLVLYMGGGMIIGSMLGAFLANL
                     FDATFVNTVYIIIALLALTLMFIKVKPSSEKSSFNKYLLVIIGLFIGIISGIVGAGGA
                     FIIIPILLVLFKLPMNTVVANSIVIAFISSIGAFVIKLIQGYIPLYDALFLIIGSIIF
                     APIGLKLSKKVPNIIQKWIISILIVFAIVQLIL"
     gene            7951..8052
                     /locus_tag="O0293_001965"
     CDS             7951..8052
                     /locus_tag="O0293_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280844.1"
                     /translation="MMKYPYMLININLSTITKNDKRFNATDDRINII"
     gene            8068..8520
                     /locus_tag="O0293_001966"
                     /pseudo
     CDS             8068..8520
                     /locus_tag="O0293_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
BASE COUNT         2816 a         1466 c         1304 g         3035 t
ORIGIN      
        1 aaaactttcg tctgtacaat ttggtttttc agatatgtta tatggcattg gaggattatg
       61 tgcaggttta atttcagcta ttctttcgaa gaaaatctca actaaagtat tgatattttt
      121 gttatatttc atattagtca taaattcagc actatttatt tggataaact cagcatttta
      181 cttattcata ggatcattta tactaggcta ctcaatttca tcaatcagaa tttatatgaa
      241 tacagctata atgaacactg tttcagataa atatgtcggt cgctcattta cgatatggac
      301 gtcaatttca ttgttactgc aaagtttaat tgctccattt ttaggaagat ggattaatga
      361 aattaatgat aaattcggtt tctatattat actcatttta tccttactca tatttgttac
      421 actgctgctt gttaacaaaa caggcaaaat aaaatatgca cataaagaag agtgaccgtc
      481 actcttcttt aacaagcgac catttatcga tgggcttagt tctctctgca cccacacttc
      541 actacttcac tttttcaaat cattttttat ggtcttaaat aaatcagtga gatttgttgc
      601 ttcggtaaag tctagaatta atatcatttc tttagaacct ggatatggcg aaactaatgt
      661 attatcttgc aatttctgct gggcactttt agtcgccttg accaataatc tattatcata
      721 caaaccacct ataaccacgc catcataata aataatatat tctcccatca tctttcttct
      781 cttaaccgcg tttgaattca catgatttaa aaataaatca tgtacatctt tcttagtcgc
      841 cattgtaatc gctccttcag ttttatgttt aatcacattc gtattaatga ttcatttcgt
      901 gttgctctta attttatcta taattatatg cgtagttaaa atcaaaccta tggaaaagaa
      961 aataatgata acaatgttaa gaaatatagt tataaaatta tagtttggaa ggtatgcgaa
     1021 tagcagaaaa ataggtatcg caaaaaataa atcccaccaa cctaaacttt ttaaagaatg
     1081 ctttaaacct tccataatat cacctttata aatttgtctt tgttataaga taactaaaaa
     1141 atcgctttac tgtaaaagta gccaaagaaa attctgaatc atattcataa gtagtgtatc
     1201 attaataatg aacaatttaa tactataatc cttgatcttt gtattgatca acttaccaca
     1261 acatttattt tagactactc ttagacttcc ctttcaaatg gttgcatcta ttgaaattcc
     1321 ttttgtataa gttaggcttt tgtggtaata tcatcatgca taaaaaatcg agatactaat
     1381 tataaagagg gtataaatat attatgaaag aaaatttttg gagtgaatta ccacgtccat
     1441 tttttatttt ggcgccaatg gaagacgtta cagatatcgt ctttcgacac gttgtaagtg
     1501 aagcagctag accggatgtg tttttcactg aatttacaaa tactgaaagc ttttgccacc
     1561 ctgaaggcat acatagtgtg cgcggacgct taacttttag tgaagatgaa cagccgatgg
     1621 tcgctcatat atggggagat aagccagaac agttccgtga aacgagtatt caattagcta
     1681 aaatgggctt taaaggcata gacttaaata tgggatgtcc tgtagcaaat gttgctaaaa
     1741 agggtaaggg ttccggctta atcttaagac ctgacgttgc tgccgaaatt attcaagcga
     1801 ctaaagcagg tgggcttccg gtaagtgtta aaacacgcct tggctactat gaaatcgatg
     1861 aatggaaaga ttggttgaag cacgtcttcg aacaagacat tgccaattta tctattcatc
     1921 ttcgtacacg taaagaaatg agtaaagtag atgcacattg ggaattaatc gaagctatta
     1981 aaaatttacg tgacgaaatt gcaccaaata cattgttaac aattaacggt gatattcccg
     2041 atagaaaaac aggacttgaa ctggcagaaa aatatggcat tgatggcgtc atgattggta
     2101 gaggcatttt ccacaatcca ttcgcttttg aaaaagaacc acgcgaacac acaagcaagg
     2161 aactattaga tctattgaga ttgcatttat cattgtttaa caaatatgaa aaagatgaaa
     2221 tacgacaatt caagagcttg cgtagattct ttaaaatcta tgtgcgtggc ataagaggcg
     2281 ctagcgaact tcgacatcaa ttgatgaaca cacaatcaat tgcagaagca cgagcactac
     2341 tcgatgaatt tgaagcccaa atggacgaag acgttaaaat tgaattatag tatgagtaat
     2401 aaagtttatg gatgatattt cccaatttaa cacggattga acactttcta cttaggtatt
     2461 atcttggttt tcctgatagg ataactcccg atgatgcctt tttacttatg tcacttatcc
     2521 ttttggaaat ggtgcaaaat taaaaataca tcaggatatc gtttaactca gaaaaaaact
     2581 ccaatcaaaa aaaatgttta caacacatta tgaaattaaa attgctctta gtgttcaaca
     2641 gacaaagtta ttgaaattac tattggaggt acataccaag ctatcatttt gagtacacgt
     2701 ggtttgaata tctggcaaga aatggaaatt ggtaatgtca gtcataaagt cgctaaacgt
     2761 gctcaaatac cattaattat ttttaaataa tttagaggat gaggtataaa cctttaaaaa
     2821 acagcagtga gatgattttc aattagaaaa tatcttactg ctgtttctat tttcataatc
     2881 attcttattg aatgacttca tcgtgatcat ttaaaacagt aacaactttt tcaatcttat
     2941 actatttaga gctaaaaatt cacgacatat aaacacaaca ctgacacgtc atttaaatac
     3001 ttaatctttt atagtaaaag atttttcatt aaaaatttat tgttttctgt actttattat
     3061 attaatcctt ttagcatgcc gtaccaatac attttaggta ataaatcttt cttaaatata
     3121 tacatacttc tacgttcttt ggcctgatta aacggcattg tttctttagt atttttatta
     3181 taatcaaact ctgcaagtat taacctatta tatccagtaa caatagggca tgaagtataa
     3241 ccatcataat gatgcgttaa catttgatta ttcatcactt gcaataaatt attagcgacg
     3301 ataggtgctt gcttacgaat agctgcgcct gtttttgaag taggtacatt tgaagcatca
     3361 ccaagtgcaa atacattaga gtagctttta tgctgtaatg tggttgggtt aacatctacc
     3421 caaccctcac tatctgaaag tgtactttct tttactacat ctaagggacc cataggtggt
     3481 gttacatgta acatatcata acttattgtt tttctatcgt atgctttgat atgttcgaat
     3541 gtagccactt ttttgtcacc gtcgatttca acaaggttat aattataatt gactgttata
     3601 tttctttctt caacaatcct ttctaattct ttattatatt ttcctacgtc aaataaagca
     3661 tcttttggcg ttgcatatat cacattagcg ttagaacgga ttttatgttt cctaaaataa
     3721 tcttcagcta aatacataat tttcataggc gcacctccac actttatagg agtgtttgga
     3781 tgcgtaaaaa tggcatttcc ttgtttaaaa ttagaaattt ggttccaagt ttcgttaaca
     3841 tagtcaggtg aatagttaga gcaaacacca tttttaccta tattttcttt tagtccttta
     3901 attgaagacc aattaatctg taatcctgga gctactacta aaaaatcata ataaacgact
     3961 gtattatctc ctaaaataac gctattattt tcaggttaaa aacttgaaac agcctgtttt
     4021 atccagttag caccttcagg tataacactt tccatatctt tacgagaact tttcaaacta
     4081 gatacccctg caccaaccaa cgtccataat ggttgatagt aatgatggtc tgctggatct
     4141 ataattgcta ttttctcttt taagttttga ttttttctta atagtcttga tgctacggta
     4201 acacctgctg taccgccacc aataataaca atttgataat gcttattcat tttttccaca
     4261 tccttaaatt attttaatga ttctggaaaa tcttgatagc cttctctaat attcaccaca
     4321 ttttcaaaac ctttgctttc caatataccc actgcaattg aacttctaac acctgactga
     4381 caatgtacat atattttatc ctctttatta aaaggaatat tttcatttaa taatttacca
     4441 tgcggaatat taactgcttg atctaagtgt ccattattcc actcttcatc attacgtacg
     4501 tctaatacat gttcttcttt acctgtcata tcagcgctat gaacggactg ggttgaaatg
     4561 cctgattttg gcaaacgata gcctgctacc ttatcaaacc caattaattg taaagtgtgt
     4621 ttcgctttct caacagtaga tttatctcca attaaatcta tatctttttc aaaatctaag
     4681 taccaaccaa tttgattaat aaagtttttg ttgtatggta tattgattgt tccttttgtg
     4741 tggccaccgt gaaaggcctc tttgctacga agatcaaatg ctactctctt attatctaaa
     4801 ctaggataaa catcatatga ttgatataaa ttcataccaa actgattaac ttgtttcatt
     4861 tgtgcaaaat gatgcggtgg tgctggttga tttgatgtta atgattcaat aaatttagtc
     4921 tcatcaattt cattaaatgc ccagttatta attttctcat aacctattgt agatataggt
     4981 atggcaccta atgctttacc acaagggctt ccagcaccat gacccggcca gatttgaaca
     5041 tagtctggta aatttttaat attttgaacg gactcataca tttgtttcgc gctaatttct
     5101 gtagaaccct ttatttgaac agatttttct aataaatcag gtctacctat atcaccaaca
     5161 aaaataaagt caccactaaa taatcccatc ggaacacttg aaccaccacc taaatcagtg
     5221 agtaaaaagc taatactttc aggcgtgtgt cctggagtat gcagaacttc taatttaaca
     5281 ttgcctactt gaatgatatc tccatgttta acaaattgtg tttttgatgg catattttta
     5341 taccctaatg catcttcacc ttcgccagac acatatatat ttgcatttaa gcgtttagcc
     5401 acatcacgaa ttcctgaagc aaaatcagca tgaatatgtg tttctgtagc ttgtgtaatt
     5461 gttaaacctt cagaatctgc aacttctata tatttcgata aatcacgaac agggtctatt
     5521 attattgcct ctcctgtacg ttgacaaccc actaaatatg atgcttgaga taaatgatta
     5581 tcgtaaaact gtttaaaaaa catgataaca actcctttta ttaatagatt taattagata
     5641 aataaattat gattcgcatt ttcagtagca ccaatataag tgcctacacc accgtactca
     5701 acttcatctc taagttcttc tttctgaata cccatgacat ccatactcat cgtacaagcg
     5761 attaatttga tattttgctc aatagcttga ttgataagtg ttggtaatga atcaacattt
     5821 ttctttttca ttacgtagcg catcatcata tttcctaaac caaacatatt cattttggaa
     5881 agaggcattc gtatattctt tttgggcaac attaaatcaa acatttttgc aataccttgc
     5941 tttttaacat taactgtttg cacttttttt aatgcattaa gcccccaaaa agtaaagaag
     6001 atagttacat cttttccagc agctctagca ccatttgcaa taatcaacgc tgctacagct
     6061 ttgtctaatt ctccactaaa taatacaatt gtagtacctt tagcagaata atttatatct
     6121 aaatcttttg ctttttcttt ttgaataatc gcattaattc cattgttatt ttcatcaagc
     6181 ttaactaaag tatgccttgt ttgtttcacc caacttttaa tgtcactagg gaatccaggg
     6241 tctgtgactt tgacttcaat ttggtcacct acttcaatat tcttcatttc ttgactaatt
     6301 tttacaattg gccctggaca ttgaagacca cgatagttaa attgtttacg atctgcttta
     6361 atttctatac ttttatattc ttcttgtgta tcataactat cgttttcttc ttcataagct
     6421 ttataaccac catcaagatt tataacgtta taaccttgtt tagctaaata tttactagca
     6481 ttagcacttc tgttaccact tctacagatt acataatagg ttttattttt ttctttattg
     6541 aatgactcaa tctcatcaac aggatgaagt atggaaccat tgatatgtcc taatgcatac
     6601 tcctcttctg ttctaacgtc aattaattgc ccttgcttgg ctagtttttc caattctgct
     6661 ttactaaatt catcgataaa cttttctccg tattgtttca taataacctc cctaaaaata
     6721 cctgtagggg tattttaaac agttggatgg aacttgtcaa atacctctag gggtatttga
     6781 cacatttgtg tttatctatt aatatatttt tattcaatta aaagtggagg tatgactatg
     6841 aattatgata aaaaaatgat taatcgtatt aatagaatac aagggcaact aaatggaatt
     6901 attaaaatga tggaggaagg aaaagactgt aaagatgtca ttacacaaat aagtgcatca
     6961 aagagttcac tccaacgctt gatgggtatc attattagtg agaatttaat agaatgtgta
     7021 aaagcagctg cggatgatga agaaagctcc caagagttaa ttaatgaagc tgtaaactta
     7081 ttggtgaaaa gtaagtaatg gatattgtta atatagttat catgttactc attggggtat
     7141 tcggtgggtt catatctggt ctagtaggcg taggtggtgc tatcattatt tatccagcta
     7201 ttttattatt accaccatta ttcggagccc ctgcatatag cgcatatatc gcatcaggtc
     7261 ttacttctag ccaagtcttt ttcagtacat taagtggctc attaaaagct cgaaaaaaaa
     7321 cagagttctc gccacaatta gttctttata tgggtggcgg catgattata gggagtatgt
     7381 taggtgcatt tctagcaaat ttatttgatg ctacatttgt aaatacggta tatatcatta
     7441 ttgctttact tgcattaaca ttaatgttta ttaaagtgaa acctagttcc gaaaagtctt
     7501 catttaataa atatttatta gttatcattg gccttttcat tggtattatt tcaggtatag
     7561 taggtgcagg tggtgccttc attattattc ctatattatt ggttttattc aaattaccaa
     7621 tgaatactgt agtcgctaat agtatagtaa ttgcttttat atcttcaatt ggtgcttttg
     7681 tcatcaaact tattcaaggt tatatccctt tatatgacgc cttattcctt ataataggga
     7741 gtattatatt tgctccaata gggttaaaat taagtaaaaa agtccctaac ataatacaaa
     7801 aatggattat cagtatttta atcgtttttg ctatagttca actcatttta taaaactaat
     7861 ccttttaact cagacagcaa tttcccgaaa aataatacct cttatttaaa caaatatgta
     7921 tgcagttcgg atttatataa ataaaaattt ttgatgaaat atccatatat gcttataaat
     7981 ataaatttat caactataac taaaaatgat aaaagattta acgccactga tgaccgaatt
     8041 aatattattt aagtttttat caaacgcatg agtaaagtag atgcacattg ggaattaatc
     8101 gaagctatta aaaatttacg tgacgaaatt gcaccaaaca cattgttaac aattaacgat
     8161 gatattcccg atagaaaaac aggacttgaa cttgcagaaa aatatggtat tgatggcatc
     8221 atgattggta gaggcatttt ccacaatcca ttcacttttg aaaaagaacc acgcgaacac
     8281 acaagcaaag aactattaga tttgttgaga ttgcatctat cattgtttaa caaatatgag
     8341 aaagatgaaa tacgacaatt caagagtttg cgtagattct ttaaaatcta tgtgcgtggc
     8401 ataagaggcg ctagcgaact tcgccatcaa ttgatgaaca cacaatcaat tgcagaagta
     8461 cgagcactac tagatgaatt tgaagcccaa atggacgaag acgttaaaat tgaattatag
     8521 tatgagtaat aagggaaagg aggaatttaa gtttcctcct ttgtttgtgt gaaagtcttt
     8581 taggtatgaa ctagggtgat ttaaaataaa agtttaattt c
//
LOCUS       DAKTIO010000117         8537 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.117, whole genome shotgun sequence.
ACCESSION   DAKTIO010000117 DAKTIO010000000
VERSION     DAKTIO010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8537
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.117"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            549..1844
                     /gene="purB"
                     /locus_tag="O0293_001967"
     CDS             549..1844
                     /gene="purB"
                     /locus_tag="O0293_001967"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCY0280845.1"
                     /translation="MIERYSREEMSNIWTDQNRYEAWLEVEILACEAWSELGHIPKAD
                     VQKIRQNAKVNVERAQEIEQETRHDVVAFTRQVSETLGEERKWVHYGLTSTDVVDTAL
                     SFVIKQANDIIEKDLERFIDVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTE
                     MQRNLQRFKQVREEIEVGKMSGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDR
                     HAYYIATLALIATSLEKFAVEIRNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSEN
                     ITGISRVIRGYITTAYENVPLWHERDISHSSAERIMLPDVTIALDYALNRFTNIVDRL
                     TVFEDNMRNNIDKTFGLIFSQRVLLALINKGMVREEAYDKVQPKAMISWETKTPFREL
                     IEQDESITSVLTKEELDECFDPKHHLNQVDTIFERAGLA"
     gene            1953..2255
                     /locus_tag="O0293_001968"
     CDS             1953..2255
                     /locus_tag="O0293_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280846.1"
                     /translation="MQIEKLRGAALDELFDAILTLENREECYQFFDDLCTVNEIQSLS
                     QRLQVAKMIKQGYTYATIEQESGASTATISRVKRSLQWGNDAYTMILDRMNIETNE"
     gene            2427..3119
                     /locus_tag="O0293_001969"
     CDS             2427..3119
                     /locus_tag="O0293_001969"
                     /EC_number="2.5.1.n9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenylglyceryl phosphate synthase"
                     /protein_id="HCY0280847.1"
                     /translation="MYDIKKWRHIFKLDPAKHISDDDLDAICMSQTDAIMIGGTDDVT
                     EDNVIHLMSRVRRYPLPLVLEISNIESVMPGFDFYFVPTVLNSTDVVFHNGTLLEALK
                     TYGHSIDFEEVIFEGYVVCNADSKMAKHTKANTDLTTEDLEAYAQMVNHMYRLPVMYI
                     EYSGIYGDVSKVQAVSEHLTETQLFYGGGISSEQQATEMAAIADTIIVGDIIYKDIKK
                     ALKTVKIKESSK"
     gene            3116..5308
                     /gene="pcrA"
                     /locus_tag="O0293_001970"
     CDS             3116..5308
                     /gene="pcrA"
                     /locus_tag="O0293_001970"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HCY0280848.1"
                     /translation="MNALLNHMNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     LDEKDVSPYNVLAITFTNKAAREMKERVQKLVGDQAEVIWMSTFHSMCVRILRRDADR
                     IGIERNFTIIDPTDQKSVIKDVLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKE
                     ATDYHSQMVATVYSGYQRQLSRNEALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHV
                     DEYQDTNKAQYTLVKLLASKFKNLCVVGDSDQSIYGWRGADIQNILSFEKDYPEANTI
                     FLEQNYRSTKTILNAANEVIKNNSERKPKGLWTANTNGEKIHYYEAMTERDEAEFVIR
                     EIMKHQRNGKKYQDMAILYRTNAQSRVLEETFMKSNMPYTMVGGQKFYDRKEIKDLLS
                     YLRIIANSNDDISLQRIINVPKRGVGPSSVEKVQNYALQNNISMFDALGEADFIGLSK
                     KVTQECLNFYELIQSLIKEQEFLEIHEIVDEVLQKSGYREMLERENTLESRSRLENID
                     EFMSVPKDYEENTPLEEQSLINFLTDLSLVADIDEADTENGVTLMTMHSAKGLEFPIV
                     FIMGMEESLFPHIRAIKSEDDHEMQEERRICYVAITRAEEVLYITHATSRMLFGRPQS
                     NMPSRFLKEIPESLLENHSSGKRQTIQPKAKPFAKRGFSQRTTSTKKQVLSSDWNVGD
                     KVMHKAWGEGMVSNVNEKNGSIELDIIFKSQGPKRLLAQFAPIEKKED"
     gene            5312..7315
                     /gene="ligA"
                     /locus_tag="O0293_001971"
     CDS             5312..7315
                     /gene="ligA"
                     /locus_tag="O0293_001971"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCY0280849.1"
                     /translation="MADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIK
                     IEEEHPEYKTVDSPTVRVGGEAQASFNKVNHDTPMLSLGNAFNEDDLRKFDQRIREQI
                     GNVEYMCELKIDGLAVSLKYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPL
                     NVEVRGEAYMPRRSFLRLNEEKEKNDEQLFANPRNAAAGSLRQLDSKLTAKRKLSVFI
                     YSVNDFTDFNARSQSEALDELDKLGFTTNKNRARVNNIDGVLEYIEKWTSQRESLPYD
                     IDGIVIKVNDLDQQDEMGFTQKSPRWAIAYKFPAEEVVTKLLDIELSIGRTGVVTPTA
                     ILEPVKVAGTTVSRASLHNEDLIHDRDIRIGDSVVVKKAGDIIPEVVRSIPERRPEDA
                     VTYHMPTHCPSCGHELVRIEGEVALRCINPKCQAQLVEGLIHFVSRQAMNIDGLGTKI
                     IQQLYQSELIKDVADIFYLTEEDLLPLDRMGQKKVDNLLAAIQQAKDNSLENLLFGLG
                     IRHLGVKASQVLAEKYETIDRLLTVTEAELVEIHDIGDKVAQSVVTYLENEDIRALIQ
                     KLKDKHVNMIYKGIKTSDIEGHPEFSGKTIVLTGKLHQMTRNEASKWLASQGAKVTSS
                     VTKNTDVVIAGEDAGSKLTKAQSLGIEIWTEQQFVDKQNELNS"
     gene            7328..8527
                     /locus_tag="O0293_001972"
     CDS             7328..8527
                     /locus_tag="O0293_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CamS family sex pheromone protein"
                     /protein_id="HCY0280850.1"
                     /translation="MKRTLVLLITAIFILAACGNHKDDQAGKDNQKHNNSSNQVKEIA
                     TDKNVQGDNYRTLLPFKESQARGLLQDNMANSYNGGDFEDGLLNLSKEVFPTDKYLYQ
                     DGQFLDKKTINAYLNPKYTKREIDKMSEKDKKDKKANENLGLNPSHEGETDPEKIAEK
                     SPAYLSNILEQDFYGGGDTKGKNIKGMTIGLAMNSVYYYKKEKDGSTFSKKLDDSEVK
                     KQGKQMASEILSRLRENDDLKDIPIHFAIYKQSSEDSITPGEFITQATAEKSQTKLNE
                     WHNINEKSALLPSSTAADYDENLNNNFKQFNDNLQSYFSNFTQAVGKVKFVDKKPQRL
                     VVDLPIDYYGQAETIGITQYVTEQANKYFDKIDNYEIRIKDGNQPRALISKTKDDKEP
                     QVHIYSN"
BASE COUNT         3256 a         1163 c         1635 g         2483 t
ORIGIN      
        1 tcaatattaa tgtcttatat ttaaaatgcc acacctatgc aatagaatat atattctttt
       61 tgtgagtaga aaatttgtta tattagaagc gaataatttt aaaacatatt atgaaatgta
      121 agaagaaata ttatgaaact aggaaaagta gattgttgaa aattccatac aataggagaa
      181 gtaaagtgaa taaattctgg tattactaaa gctattgatt gtcctaattg taataaaaag
      241 ttggatagtg tagttgaggg gagaaaaact aaaatgcaaa aatatattct caatggatca
      301 ttataaatac caattagatt aaagtattgc gaacatttac aattagaaat cagtattaat
      361 ctgccataga taattgtatt aatgtaaaaa ataaaataga atgagaatat acggaaattt
      421 tttatacatt tataaccaat tctgttaaac tgtgaagagt agagttaaaa acgaactttt
      481 tgtttatttt tattaaaaaa tgttctgttt ttgaaaattc gagtatacat acgataggag
      541 ttaaaaaaat gattgaacgc tattctagag aagaaatgtc taatatttgg actgatcaaa
      601 atcgctatga agcatggtta gaagtggaaa ttttagcatg tgaagcatgg agtgaattag
      661 gacatatacc gaaagctgac gtacaaaaaa ttcgtcaaaa cgcaaaggta aatgtcgaac
      721 gtgcacaaga aattgaacaa gaaacgcgcc atgatgttgt agcctttact agacaagttt
      781 ctgaaacgct aggtgaagaa cgtaagtggg tacattatgg tttaacttct actgatgttg
      841 tagatacagc tttaagtttc gttattaaac aagcaaatga cattattgaa aaagatttag
      901 aaagatttat cgacgtttta gctgaaaaag caaaaaatta taaatataca ttgatgatgg
      961 gacgtacaca tggagtgcat gcagaaccaa ctacatttgg tgtcaaaatg gcattgtggt
     1021 acacggaaat gcaacgtaac ttacaacgtt tcaaacaagt aagagaagaa attgaagtag
     1081 ggaaaatgag tggtgcagta ggtacttttg ctaacattcc tcctgaaata gaaagttatg
     1141 tgtgtaaaca tttaggtatt ggcacagcgc ctgtatcaac gcaaacattg caacgcgatc
     1201 gacatgcata ctatattgcg acattagcat taatcgcgac atcactagaa aaatttgccg
     1261 ttgaaatacg taaccttcaa aaaaccgaaa cgagagaagt tgaagaagca tttgcaaaag
     1321 gccaaaaagg ttcatctgca atgcctcata aacgaaatcc aattggttct gaaaatatca
     1381 caggtatttc aagagtcatt cgtggttata ttactacagc ttatgagaat gtgccattat
     1441 ggcatgaaag agatatttct cattcttctg ctgagcgtat tatgttacca gatgttacaa
     1501 tagcactaga ttatgcattg aatcgtttca caaatattgt ggaccgttta actgtatttg
     1561 aagataatat gcgtaataac atagataaaa catttggtct gattttctca caacgtgtct
     1621 tattagcatt aattaataaa ggtatggttc gtgaagaagc atacgacaaa gtacaaccaa
     1681 aagcgatgat atcttgggaa acgaaaacac cgttccgtga attaatcgaa caagatgagt
     1741 ctattacaag tgttttaaca aaagaagaat tggatgaatg tttcgatcct aaacatcatt
     1801 tgaatcaagt tgacactatt ttcgaacgcg ctggattagc ataaaattaa ataaagtaac
     1861 gattcagatt taataaaata taaatcgcta cactttaata gattaatgcg ttaaaatgac
     1921 ataaagaata aaattatagg ggttgtttcg atatgcaaat tgaaaaatta cgaggtgcag
     1981 cgttagatga attgtttgat gcaatactaa cgttagaaaa tagagaagaa tgttaccaat
     2041 ttttcgatga tttgtgtact gtaaatgaaa ttcaatcact gtctcaaaga ttacaagttg
     2101 ctaaaatgat taagcaaggt tatacctatg caacgattga acaagaatct ggagcatcga
     2161 ctgcaacgat ttctagagtg aagcgttcat tacaatgggg taatgatgct tatacaatga
     2221 ttttagatcg tatgaatatt gaaacaaatg aataataaat gaacgataaa caatggttat
     2281 ctatctgcac taataaggta gataatcatt gttttttcac gaaaaaattt acagagtaaa
     2341 agaacttaaa tttcatatta agtctttaga actcgacact taaaaatgct ataatcatat
     2401 gtatgttaaa aaaggagttt cggaaaatgt atgacattaa aaaatggcgc catattttta
     2461 aattagaccc agctaaacat atttcagatg atgatttaga tgcgatttgt atgtctcaaa
     2521 cagatgcaat tatgattggt ggaactgatg acgttactga agataatgtc attcatttaa
     2581 tgagcagagt aagaagatac ccattgcctt tagtacttga aatatcaaac atcgaaagtg
     2641 taatgcctgg ttttgatttt tattttgtac ctacagtact gaacagtaca gatgttgtat
     2701 ttcacaatgg tacattatta gaagcgctta aaacatatgg acatagtata gattttgagg
     2761 aagtaatatt tgaagggtat gtcgtgtgca atgctgatag caaaatggca aaacatacca
     2821 aagcaaatac agatttaaca acagaagatt tagaagcata tgcccaaatg gtcaatcata
     2881 tgtatcgatt accggttatg tatatagagt atagtggcat ttatggcgac gtatcaaagg
     2941 ttcaagctgt ctcagaacat ctaacagaaa cgcaactttt ttatggtggc ggtatttcct
     3001 cagaacaaca agcgacagag atggcagcta ttgcagatac aattatcgtc ggtgatatta
     3061 tttataaaga tattaaaaaa gctttaaaaa cagtaaaaat aaaggagtct agtaaatgaa
     3121 tgcgttatta aatcatatga atacagagca aagtgaagct gtaaagacaa cagaaggacc
     3181 attgttaatt atggcaggtg ctggttcagg gaagacacgt gttttaacac atagaattgc
     3241 ttatttatta gacgaaaaag atgtctcacc atacaatgtt ttggctatta cttttacaaa
     3301 taaagctgca agagaaatga aagaacgtgt tcaaaaatta gtaggtgatc aagcagaagt
     3361 tatttggatg tcaacattcc actcaatgtg tgttcgtatt ttacgtcgtg atgcagatcg
     3421 aattggtata gaacgcaatt ttacgataat tgatcctaca gaccaaaaat ctgttattaa
     3481 agacgtctta aaaaatgaaa atattgatag taaaaagttt gaacctcgta tgtttatcgg
     3541 tgcgatcagt aatttgaaaa atgaacttaa aacacctgca gatgctcaaa aagaagccac
     3601 agattatcac tcgcaaatgg tagcaacggt ttatagtgga tatcaacgcc aattgtcacg
     3661 taatgaagcg ttagattttg atgaccttat tatgacaacg attaacttat ttgagcgtgt
     3721 accagaagtt ctagaatatt atcagaacaa attccaatat attcatgtag atgagtatca
     3781 agatactaat aaagcacaat acacattagt taaattatta gcaagtaagt ttaaaaactt
     3841 atgtgttgta ggtgactcag atcagtcaat ttatggttgg cgtggtgctg atattcaaaa
     3901 tatcttatca tttgaaaaag actatccaga agcgaataca atctttttag agcaaaatta
     3961 tcgttcgacg aaaacgattt taaatgcggc taacgaagtg attaaaaata attctgaacg
     4021 taagccaaaa ggactgtgga ctgcaaatac gaatggtgag aaaattcatt actatgaagc
     4081 aatgacggaa cgtgatgaag cggaatttgt aatacgagaa attatgaagc atcaacgtaa
     4141 tggtaagaaa tatcaagata tggcaatttt atatagaacg aatgcacaat cacgtgtact
     4201 tgaggaaaca ttcatgaaat ctaatatgcc atacacaatg gttggtggcc aaaagttcta
     4261 tgaccgtaaa gaaatcaaag atttattaag ttatttgcgt ataattgcca atagtaatga
     4321 tgacattagt ttgcaacgta ttattaatgt tccaaaaaga ggtgtaggtc cttcatctgt
     4381 tgaaaaagtt caaaactatg cacttcaaaa caatatcagt atgtttgatg cacttggaga
     4441 agctgatttt atcggattgt caaaaaaggt gacacaagag tgtcttaatt tttacgagtt
     4501 aatccaaagc ttgataaaag aacaagaatt tttagaaatt catgaaatag ttgatgaagt
     4561 attacaaaag tctggctatc gagaaatgct tgaacgtgaa aatacattag aatctcgaag
     4621 tagattagaa aacatcgatg aatttatgtc agtaccaaaa gactatgagg aaaatacccc
     4681 attagaagaa cagtcattaa ttaacttttt aacggattta tcgttagtag ctgatattga
     4741 tgaggcagat actgaaaatg gcgtaacact aatgacgatg cactcggcta agggccttga
     4801 atttccaatt gtctttataa tggggatgga agaatcttta ttcccacata ttagagcgat
     4861 taagagtgaa gatgatcatg aaatgcaaga agaacgtcgt atttgttatg tagcaattac
     4921 aagggctgaa gaggtgttat atatcactca tgcgacatca agaatgttat ttggtcgccc
     4981 tcagtcaaat atgccatcca gatttttaaa ggaaattcca gaatcactat tagaaaatca
     5041 ttcaagtggc aaacgacaaa cgatacaacc taaggcaaaa ccttttgcta aacgcggatt
     5101 tagtcaacga acaacgtcaa cgaaaaaaca agtattgtca tctgattgga atgtaggtga
     5161 caaagtgatg cataaagcct ggggagaagg catggtgagt aatgtaaacg agaaaaatgg
     5221 ctcaatcgaa ctagatatta tctttaaatc acaagggcca aaacgtttgt tagcgcaatt
     5281 tgcaccaatt gaaaaaaagg aggattaagg gatggctgat ttatcgtctc gtgtgaacga
     5341 gttacatgat ttattaaatc aatacagtta tgaatactat gtagaggata atccatctgt
     5401 accagatagt gaatatgaca aattacttca tgaactgatt aaaatagaag aggagcatcc
     5461 tgagtataag actgtagatt ctccaacagt tagagttggc ggtgaagccc aagcctcttt
     5521 caataaagtc aaccatgaca cgccaatgtt aagtttaggg aatgcattta atgaggatga
     5581 tttgagaaaa ttcgaccaac gcatacgtga acaaattggc aacgttgaat atatgtgcga
     5641 attaaaaatt gatggcttag cagtatcatt gaaatatgtt gatggatact tcgttcaagg
     5701 tttaacacgt ggtgatggaa caacaggtga agatattacc gaaaatttaa aaacaattca
     5761 tgcgatacct ttgaaaatga aagaaccatt aaatgtagaa gttcgtggtg aagcatatat
     5821 gccgagacgt tcatttttac gattaaatga agaaaaagaa aaaaatgatg agcagttatt
     5881 tgcaaatcca agaaacgctg ctgcgggatc attaagacag ttagattcta aattaacggc
     5941 aaaacgaaag ctaagcgtat ttatatatag tgtcaatgat ttcactgatt tcaatgcgcg
     6001 ttcgcaaagt gaagcattag atgagttaga taaattaggt tttacaacga ataaaaatag
     6061 agcgcgtgta aataatatcg atggtgtttt agagtatatt gaaaaatgga caagccaaag
     6121 agagtcatta ccttatgata ttgatgggat tgttattaag gttaatgatt tagatcaaca
     6181 ggatgagatg ggattcacac aaaaatctcc tagatgggcc attgcttata aatttccagc
     6241 tgaggaagta gtaactaaat tattagatat tgaattaagt attggacgaa caggtgtagt
     6301 cacacctact gctattttag aaccagtaaa agtagctggt acaactgtat caagagcatc
     6361 tttgcacaat gaggatttaa ttcatgacag agatattcga attggtgata gtgttgtagt
     6421 gaaaaaagca ggtgacatca tacctgaagt tgtacgtagt attccagaac gtagacctga
     6481 ggatgctgtc acatatcata tgccaaccca ttgtccaagt tgtggacatg aattagtacg
     6541 tattgaaggc gaagtagcac ttcgttgcat taatccaaaa tgccaagcac aacttgttga
     6601 aggattgatt cactttgtat caagacaagc catgaatatt gatggtttag gcactaaaat
     6661 tattcaacag ctttatcaaa gcgaattaat taaagatgtt gctgatattt tctatttaac
     6721 agaagaagat ttattacctt tagacagaat ggggcagaaa aaagttgata atttattagc
     6781 tgccattcaa caagctaagg acaactcttt agaaaattta ttatttggtc taggtattag
     6841 gcatttaggt gttaaagcga gccaagtgtt agcagaaaaa tatgaaacga tagatcgatt
     6901 actaacggta actgaagcgg aattagtaga aattcatgat ataggtgata aagtagcaca
     6961 atctgtagtt acttatttag aaaatgaaga tattcgtgct ttaattcaaa aattaaaaga
     7021 taaacatgtt aatatgattt ataaaggtat caaaacatca gatattgaag gacatcctga
     7081 atttagtggt aaaacgatag tactgactgg taagctacat caaatgacac gcaatgaagc
     7141 atctaaatgg cttgcatcac aaggtgctaa agttacaagt agcgttacta aaaatacaga
     7201 tgtcgttatt gctggtgaag atgcaggttc aaaattaaca aaagcacaaa gtttaggtat
     7261 tgaaatttgg acagagcaac aatttgtaga taagcaaaat gaattaaata gttagagggg
     7321 tatgtcgatg aagcgtacat tagtattatt gattacagct atctttatac tcgctgcttg
     7381 tggtaaccat aaggatgacc aggctggaaa agataatcaa aaacataaca atagttcaaa
     7441 tcaagtaaaa gaaattgcaa cggataaaaa tgtacaaggt gataactatc gtacattgtt
     7501 accatttaaa gaaagccagg caagaggact tttacaagat aacatggcaa atagttataa
     7561 tggcggcgac tttgaagatg gtttattgaa cttaagtaaa gaagtatttc caacagataa
     7621 atatttgtat caagatggtc aatttttgga caagaaaaca attaatgcct atttaaatcc
     7681 taagtataca aaacgtgaaa tcgataaaat gtctgaaaaa gataaaaaag acaagaaagc
     7741 gaatgaaaat ttaggactta atccatcaca cgaaggtgaa acagatcctg aaaagattgc
     7801 agaaaaatca ccagcctatt tatctaacat tttagagcaa gatttttatg gtggtggaga
     7861 tacaaaaggt aagaatatta aaggtatgac gattggttta gctatgaata gtgtttatta
     7921 ctataaaaaa gaaaaagatg gatcgacttt tagtaaaaaa ctagatgata gcgaagttaa
     7981 aaagcaaggt aaacaaatgg ctagtgagat attatcaagg ttacgtgaaa atgatgattt
     8041 aaaagatata ccaattcatt ttgcaattta taagcaatca agtgaagatt caatcacacc
     8101 aggtgaattt atcactcaag cgactgcaga aaagagtcaa acaaagctta atgaatggca
     8161 taatatcaat gaaaaatcag ctttattacc ttcttcaaca gcagcagatt atgatgaaaa
     8221 tttaaataat aatttcaagc aatttaatga taatttgcaa tcatattttt ctaatttcac
     8281 acaagcagta gggaaagtta aatttgttga taaaaagcca caacgattag tagtagattt
     8341 accaatcgat tactatggac aagctgaaac aattggtatt acacagtacg ttactgaaca
     8401 agcgaataaa tatttcgata aaatcgataa ctatgaaatt cgtattaaag atggtaacca
     8461 accacgtgct ttaattagta agacaaaaga tgacaaagaa ccgcaagttc atatttacag
     8521 taattaattg taaatta
//
LOCUS       DAKTIO010000118         8408 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.118, whole genome shotgun sequence.
ACCESSION   DAKTIO010000118 DAKTIO010000000
VERSION     DAKTIO010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8408
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.118"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            278..3232
                     /gene="fdhF"
                     /locus_tag="O0293_001973"
     CDS             278..3232
                     /gene="fdhF"
                     /locus_tag="O0293_001973"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCY0280851.1"
                     /translation="MQEHLVVTLDGKDYLVEPGTNLLEFIKSQDTFVPSICYNESMGP
                     IQTCDTCTVEIDGKIERSCSTVIDRPMTVNTVNNDVKDAQKEALDRILEKHMLYCTVC
                     DYNNGDCEIHNTMDAWGLQHQTYEYKEKPYEKDYGPFYRYDPNQCILCGRCVEACQDI
                     EVNETIRIDWDREHPRVIWDNDVPINESSCVSCGQCATVCPCNAMMEVNMEGNAGYMT
                     DTEPGSLAAMIDLTKKAEPGYGPLFAISDSEAEMRKERIKKTKTVCTYCGVGCSFEVW
                     TKDREILKVQPSHDSPANKIATCVKGKFSWGHINSDQRLTKPLVRKNGEFHEVEWDEA
                     LNVIADNFTAIKEKHGPDALSFISSSKATNEESYLMQKLARQVIGTNNVDNCSRYCQA
                     PATKGLFRTVGHGGDSGSIEDLEKAAMSVLIGTNTAEAHPVIASRMKRAQKLFGQKIH
                     VFDIRKHEMAERADRFYQPKPGTDLAWLSAVTKYIIDHDLHDKAFIDEWVDDFDEYYK
                     SLETFTMAFAEEATGIPESELIKFAEECAKAESVVICWAMGITQQDIGSDSSTAISNL
                     LLVTGNYRRPGTGAYPLRGHNNVQGCSDMGSMPDKITGYQSIEADDIRAKFEKEYGVK
                     LNPKAGKDNHEMVEGIHDGEVHSLYLYGEDTGIVDSNINFVQAAFEKLDFMVVQDEFL
                     TFTATYADVVLPASPSLEKDGTFTNTERRIQRLYQALEPLGDSKPDWKIFQAIANRLG
                     FDWNYKHPSEIMDEVARLTPLYAGVSYDRLEGFNSLQWPVQPDGTDEPILYLEGFNFD
                     NGKAKLFPLSFDNYFKQDEIYDIHVNNGRLLEHFHEGNMTYQTPMIKYKVPRAFVEIS
                     PELAEDRGIHEGAEVKLISETGEAVLQVHVTDRVKGKEIYIPLNNDAMENGDLGAINL
                     LTNSDVDQYTDTPSYKRTSCRLEVITKRGKSPLNPNNFRVNKKRQPQYSVQVQKKWER
                     SDYVFPGNQVDK"
     gene            3232..3705
                     /locus_tag="O0293_001974"
     CDS             3232..3705
                     /locus_tag="O0293_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1641 domain-containing protein"
                     /protein_id="HCY0280852.1"
                     /translation="MAERISSKIRRLEKSEEQIKLESLNEVTEAIAANKDSILKAIKL
                     IKTLDDAKLLDALNGAIRGRQVIINKFAVELNKDIYTGLLSNMASMVFLLGELNVSDL
                     SDFLNKVNKGLHVANQASPNAKTTIRSLLGVLKDDDMNRSLTYMLNMLKGMSREE"
     gene            3933..4166
                     /locus_tag="O0293_001975"
     CDS             3933..4166
                     /locus_tag="O0293_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280853.1"
                     /translation="MLKTSAVISNTLLVIGLVCLFMMKLVLAITFFAVSLSISLVVFN
                     MMFRDRTTMKVIVNASFLIVILAIVVAYFLLSK"
     gene            4188..4964
                     /locus_tag="O0293_001976"
     CDS             4188..4964
                     /locus_tag="O0293_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HCY0280854.1"
                     /translation="MKKNFKLRISTLLLIVILVVFAVLLIVNETKLFKNDVNYSFDEA
                     VSMQQGKGIVQTKEEDGKFVEANNNEIAKAMTISHKDNDMKYMDITEKVPMSESEVNQ
                     LLKGKGILENRGKVFLEAQEKYEVNVIYLVSHALVETGNGKSELAKGIKDGKKRYYNF
                     FGIGAFDSSAVRSGKSYAEKEQWTSPDKAIIGGAKFIRNEYFENNQLNLYQMRWNPEN
                     PAQHQYASDIRWADKIAKLMDKSYKQFGIKKDDIRQTYYK"
     gene            5275..6399
                     /locus_tag="O0293_001977"
     CDS             5275..6399
                     /locus_tag="O0293_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HCY0280855.1"
                     /translation="MKIAIIGAGIGGLTAAALLQEQGHTIKVFEKNESVKEIGAGIGI
                     GDNVLKKLGNHDLAKGIKNAGQILSTMTVLDDKDRLLTTVKLKSNTLNVTLPRQTLID
                     IIKSYVKDDAIFTNHEVTHIDNETDKVTIHFAEQESEAFDLCIGADGIHSKVRQSVNA
                     DSKVLYQGYTCFRGLIDDIDLKHPDCAKEYWGRKGRVGIVPLLNNQAYWFITINSKEN
                     NHKYSSFGKPHLQAYFNHYPNEVREILDKQSETGILLHNIYDLKPLKSFVYGRTILLG
                     DAAHATTPNMGQGAGQAMEDAIVLVNCFNAYDFEKALQRYDKIRVKHTAKVIKRSRKI
                     GKIAQYRSRLFVAVRNRIMKMMPNALAAGQTKFLYKSKEK"
     gene            complement(6491..7444)
                     /locus_tag="O0293_001978"
     CDS             complement(6491..7444)
                     /locus_tag="O0293_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HCY0280856.1"
                     /translation="MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKN
                     ATALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNIDIEYAREKSIDVTNTPKASTNA
                     TADLTIGLVLAVARRIVEGDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAV
                     ARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLID
                     TEQFKMMKSTAYLINASRGPIVHEQALVQALKDNEIEGAALDVYEFEPDITDDLKSLN
                     NVVLTPHIGNATFEARDMMSKIVANAAISAVQGEKPQFVVN"
     gene            complement(7806..8306)
                     /locus_tag="O0293_001979"
     CDS             complement(7806..8306)
                     /locus_tag="O0293_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0280857.1"
                     /translation="MKKLVTATTLTAGIGTALVGQAHHADAAENYTNYNNYNYNTTQT
                     TTTTTTTTTTSSISHSGNLYTAGQCTWYVYDKVGGEIGSTWGNANNWAAAAQGAGFTV
                     NHTPSKGAILQSSEGPFGHVAYVESVNSDGSVTISEMNYSGGPFSVSSRTISASEAGN
                     YNYIHI"
BASE COUNT         2963 a         1206 c         1607 g         2632 t
ORIGIN      
        1 aagatatggc aaaaagcgtg aaacagataa ttggaaaact aataatgata attattttca
       61 attaaacaaa aagatgattt aatttcgcgc aaaatatttc aaataaaaat gattgtaaaa
      121 aggcaaaata caaaatttta cttaacaact agtacataaa gtaatacaat taaattaatt
      181 ctatctgaaa gatgtgtggg gcatcgttat tttaggtgga tatgagcaat ttattaaaag
      241 tcatttacgg aaaatatata tagacggggt gagtaatatg caagaacatt tggtggttac
      301 acttgatgga aaagattatc ttgtagaacc aggtacgaat ttacttgaat ttattaaatc
      361 acaagatact tttgtacctt caatttgtta taacgagtcg atgggaccaa ttcaaacatg
      421 tgatacatgt actgttgaga ttgacggtaa aattgaacgt tcatgtagta cggtgattga
      481 tcgtccaatg actgtaaata ctgtgaacaa tgatgtgaaa gatgctcaaa aagaagcgct
      541 tgatcgaatt ttagaaaagc atatgctgta ttgtacagta tgtgattata ataatggtga
      601 ttgtgaaatt cataatacga tggatgcatg ggggcttcag catcaaacgt atgaatataa
      661 agagaagcca tacgaaaaag attacggccc attttatcgt tatgatccaa atcaatgtat
      721 cttatgtggg cgttgtgtag aagcatgtca agatatcgaa gtgaatgaaa ctattagaat
      781 tgattgggat cgtgaacatc cacgtgttat ttgggataat gatgtaccga ttaatgagtc
      841 ttcatgtgta tcttgtggtc aatgtgcgac ggtatgtcca tgtaatgcca tgatggaagt
      901 gaatatggaa ggtaatgcgg gttatatgac tgatacggaa cctggttcat tagcagcaat
      961 gattgattta actaaaaaag cagaacctgg ttatggtcca ctatttgcaa tctcagattc
     1021 agaagcagaa atgcgtaaag agcgtattaa gaaaactaaa acagtgtgta cttattgtgg
     1081 tgtgggttgt tcatttgaag tttggactaa agatagagaa attttaaaag tacaaccatc
     1141 acatgattct ccagcaaata aaattgcgac ttgtgttaaa ggtaagtttt catggggaca
     1201 tattaattca gatcaacgat taactaaacc actagtaagg aaaaatggtg agttccatga
     1261 agtagaatgg gatgaagctt taaacgtcat tgcagataat tttacagcta ttaaagaaaa
     1321 gcatggccca gatgcactat cattcatttc ttcttctaaa gcgacgaatg aagaatcgta
     1381 tttaatgcaa aaattagcaa gacaagttat tggcacaaat aacgttgata actgttcaag
     1441 atattgccaa gcacctgcaa caaaaggctt atttagaacg gttggacacg gcggtgactc
     1501 aggtagtatt gaagatttag aaaaagcggc aatgtctgta ttgataggta ctaatacagc
     1561 cgaagctcat ccagttatcg catcacgcat gaaacgtgca caaaaattat ttggtcaaaa
     1621 aatacatgta tttgatatta gaaaacatga aatggcagaa cgtgcggatc gtttttatca
     1681 acctaaacca ggtacggatt tagcgtggtt aagtgcagtg actaagtata ttattgatca
     1741 tgatttacac gataaagcat ttattgatga gtgggtagat gattttgatg aatattacaa
     1801 atcattagaa acatttacaa tggcttttgc tgaagaagca acaggtattc ctgaatcaga
     1861 attgattaaa tttgctgaag aatgtgctaa agctgaatct gttgtaattt gttgggcaat
     1921 gggtattaca caacaagaca ttggtagtga ctcaagtaca gcgatttcaa acttattatt
     1981 agtaacaggt aattatcgtc gtcctggtac tggtgcgtat ccattacgtg gacataataa
     2041 tgttcaagga tgtagtgata tgggaagtat gcctgataag attactggtt atcaaagtat
     2101 tgaagcggat gatattcgcg ctaaatttga aaaagaatat ggcgttaaat tgaatccaaa
     2161 agctggtaaa gataatcatg aaatggtaga aggtatacat gacggagaag tacactcatt
     2221 gtacttatat ggtgaagata cgggtattgt ggattcaaat attaattttg tacaagctgc
     2281 gtttgaaaaa ttagatttca tggtagtcca agatgaattt ttaacattca cagcaacata
     2341 cgcagatgtt gtattgccag caagtccttc acttgaaaaa gacggtactt ttacaaatac
     2401 cgaacgtcgt attcaacgtt tataccaagc attagaacct cttggtgatt caaaacctga
     2461 ctggaaaatt ttccaagcaa ttgctaatag attaggattt gattggaatt acaagcatcc
     2521 tagtgaaatt atggatgaag tagcacgctt aacacctcta tatgctgggg taagttatga
     2581 tcgtttagaa ggattcaata gtttacaatg gccagtacaa cctgatggta ctgatgagcc
     2641 tattctatac ttagaaggat tcaattttga taatggtaaa gcaaaattat tcccattatc
     2701 atttgataat tactttaagc aagatgaaat ttatgatatt catgtaaaca atggtcgatt
     2761 gttagagcac ttccatgaag gtaatatgac ttatcaaaca ccaatgatta aatacaaagt
     2821 gccacgtgca tttgttgaaa tttctccaga gcttgctgaa gatagaggca ttcatgaagg
     2881 tgcagaagtt aagctgattt ctgaaacagg agaagcggta ttacaagttc acgtgacaga
     2941 tcgtgttaaa ggtaaagaaa tctatattcc attaaataat gatgcgatgg aaaatggaga
     3001 tctaggtgca attaacttat taactaatag tgatgttgat caatacacgg atacaccatc
     3061 ttataaacgt actagctgtc gtttggaggt aattacgaaa cgtggtaagt caccgttgaa
     3121 ccctaataat ttccgtgtca ataaaaaacg tcaaccgcag tacagtgttc aagtacagaa
     3181 aaaatgggaa cgttcagatt atgttttccc gggaaatcag gtggataaat aatggctgag
     3241 agaatatctt ctaaaattag acgcttagaa aaatcagaag aacaaattaa gttagaaagt
     3301 ttaaatgaag ttacagaggc aattgcagcg aataaagata gtatattaaa agcaatcaag
     3361 cttattaaaa cattagatga tgcaaaacta ttagatgctt taaatggtgc aattcgagga
     3421 cgtcaagtca tcataaataa atttgcagtt gaacttaata aagatattta tacagggcta
     3481 ttatctaata tggcttcaat ggtattttta ttaggtgaac tcaatgtatc agatttaagc
     3541 gacttcctaa ataaagttaa taaaggatta cacgttgcca atcaagcgag tccaaatgct
     3601 aagacaacta taagaagtct attgggcgta ttgaaagatg acgacatgaa ccgtagttta
     3661 acatacatgc ttaatatgct aaaaggtatg tctcgagaag aataacatta caatgaaaaa
     3721 gtgtttctac atgcaagcag tgtaggagca ctttttttaa tttttgtaaa aattaaaaag
     3781 ggtgtttttg atggaataaa tgaataatat tgaaaaacaa aaaaatcacg tgctattgtg
     3841 aatcaatcat cgacaggcct atgaaacatc atgtattatt atgattatgt aagttattta
     3901 aataaaaagg gtgagtgcat attaattcga gaatgttaaa aacgtcagct gtaatttcta
     3961 atacattatt agtcataggt ttagtctgtc tatttatgat gaaattagtg ttagctatta
     4021 ctttttttgc tgtttcatta tcaattagtc tagttgtctt taatatgatg ttcagagata
     4081 gaacaacgat gaaagtaatc gttaacgctt cttttttaat cgtaatatta gcgattgtag
     4141 tggcatattt tttattgtct aagtaagaaa aatgaggttt tgtctatatg aagaagaatt
     4201 tcaagttacg catttcaacg ctactattga tagttatttt agttgttttt gctgtattac
     4261 tcatcgtgaa tgaaactaaa ttgtttaaaa atgatgtgaa ttactctttt gatgaggctg
     4321 tttcaatgca acaagggaaa ggtattgtac agacaaaaga agaggatggt aaatttgttg
     4381 aagcaaataa taatgaaatt gctaaagcaa tgactatttc acataaagac aatgatatga
     4441 agtatatgga tatcacagaa aaagtgccaa tgtcggaatc tgaagttaac caattgctaa
     4501 aaggtaaggg gattttagaa aatcgaggga aagtttttct agaagctcaa gaaaaatatg
     4561 aggttaatgt catttatctt gttagccatg cattagtaga aacaggtaac ggcaaatcag
     4621 aattagcaaa aggcattaaa gatgggaaaa aacgctatta caactttttt ggtataggag
     4681 cattcgatag tagtgctgtt cgtagtggga aaagttatgc tgaaaaggaa caatggacat
     4741 caccagataa ggcgattatt ggtggtgcaa agttcattcg taatgaatat tttgaaaaca
     4801 atcaactgaa tttatatcaa atgcgatgga atccagaaaa tcctgcgcaa catcaatatg
     4861 cgagtgacat tcgctgggca gataaaattg ccaaattaat ggataaatcc tataagcagt
     4921 ttggtataaa gaaagatgat attagacaaa catattataa ataagacatc ggtgcttaaa
     4981 ggagctggaa caatttattg tttcgagctc ctttagcgca ttctgagtgt gttagttaaa
     5041 tggattttaa cctaacaaaa aacgctatat agcatcaaat atgctatatc ccacatcatt
     5101 gttacaaatg tacatgatgt aaatgaatat tgctgtctaa atgtgcatgt aatatacaat
     5161 ggtgcagata atacacttaa gtccttaaaa atgaaacgtt agttccaaga gtcattttta
     5221 aacaatagtg catgtgataa aatagaaaag aatgaaaaat atagaggtga caatatgaag
     5281 atagcaatta taggtgcagg catcggtgga ttaacagctg ctgcattatt acaagaacaa
     5341 ggtcatacta ttaaagtctt tgaaaaaaat gagtcagtta aagaaattgg cgctgggatt
     5401 ggtatcggag ataatgtgct taaaaaacta ggtaatcatg acttagctaa aggtattaaa
     5461 aatgctgggc aaatcttatc tacaatgaca gtgttagatg acaaagatcg cctgttaact
     5521 actgttaaat taaaaagtaa tacattgaat gtgacgttac cacgccaaac attaattgac
     5581 attattaaat cttatgtaaa agatgacgca atatttacaa atcatgaagt cacgcatata
     5641 gataatgaga cagataaagt taccatacat ttcgcggaac aagaaagtga agcatttgat
     5701 ttatgtattg gtgctgatgg aattcattct aaagtgagac aatctgtaaa tgctgacagt
     5761 aaagtattat atcaagggta tacatgcttt agaggtttaa ttgatgatat tgatttaaag
     5821 catccagatt gtgcaaaaga atactgggga agaaaaggaa gagtaggtat tgttccgtta
     5881 ttaaataatc aagcatattg gttcattaca attaactcga aggaaaacaa tcataaatat
     5941 agttcgtttg gtaaacctca tttgcaagca tactttaatc actatccaaa tgaagttaga
     6001 gagatcttag acaaacaaag tgaaacaggt atcttattgc ataatattta tgatttgaaa
     6061 ccactcaaat cttttgttta tggtcgtact attttactag gagatgcagc acatgcgaca
     6121 acgcctaata tggggcaagg tgctggacaa gcaatggaag atgctatcgt attagtaaat
     6181 tgttttaacg catacgactt tgaaaaagca ttacagcgtt atgataaaat acgtgtcaaa
     6241 catactgcaa aagtaattaa gcgttctaga aaaatcggta aaattgccca atatcgtagt
     6301 cgtttatttg ttgcagttag aaatcgtatt atgaaaatga tgccaaatgc attagcagct
     6361 ggacaaacta aattcttata taaatcgaaa gaaaaataat acaacaatat gaaaaccccc
     6421 gtatgttgaa acgagagctc aacatatggg ggttcttgtt tttataatgt tattataata
     6481 aattcaatta ttagttaacg acaaattgtg gtttctcacc ttgaacggca ctaattgcag
     6541 cattagcaac aattttagac atcatgtcac gtgcttcaaa tgtagcatta ccaatatgcg
     6601 gtgttaatac tacattatta agtgatttta agtcatcggt aatatctggt tcaaattcat
     6661 atacatcaag tgcagcacct tcaatttcat tatctttcaa tgcttgcact agtgcttgtt
     6721 cgtgcacgat tggaccacga gaggcattga ttaaatacgc cgtagatttc atcattttaa
     6781 attgttctgt atcaattaaa tgatgcattt taggattata agcagcgttg atagtgataa
     6841 aatctgcatt ctttaatagt gtatctaaat ctacatattt tgcaccgatt tctcgttctt
     6901 tttcttcttt gcgattaggt ccagtgtata gcacatccat gtcaaatgct cttgcacgac
     6961 gagctactgc actaccaatt tcacctaaac cgataatgcc gattgttttc ccagatactt
     7021 ctctacctct gaaaaataaa ggtgcccatc catcaaatcc agttgtacgt gataattggt
     7081 ccccttcaac aatacgacgc gctactgcaa gtactaatcc aattgttaaa tcagcagtcg
     7141 cgtttgttga tgctttaggt gtgtttgtaa catctatact tttttctcgg gcatactcga
     7201 tatcaatatt attaaaacca gcgccatagt tggcaatgat ttttaagtct ttaccagcat
     7261 cgataacatc tttatcaacg tttgtagata ataaactaat taaggcagtc gcgtttttaa
     7321 cacctttaat taaagtgtct ttatcgacta atcctttacc ttcatacatt tcaacttcaa
     7381 aatgttcttg taaaagtttt aaacctactt ctggtattgc accagcaaca taaacttttt
     7441 ccataaaaga tcactccttt tatcttagta tagtagaaga ttagacagta tacaactatg
     7501 tcatgatgtc ttgtgtatca atgatgtaag cgcgtacttt tgatggaggt gatataactt
     7561 aggcactgta gaactatgaa tattgtaatg tggaaaaact ggatcaatta aattagataa
     7621 cgtagtttta aagttaatag tattagaaaa aaataatatt ttgaatatgg gaggaaatat
     7681 aaataagtag gtggcaacga aaaatagcaa aaaaagagct tctcctataa aggaaagctc
     7741 aaagtttttt gatgacatat gtactagaat taagtttcaa gacaatatgt atcatcgtgt
     7801 ttatattaaa tatggatgta gttgtagtta cctgcttcac ttgcagaaat agttctagaa
     7861 cttactgaga aaggtccgcc actataattc atttctgaaa ttgtaactga accatcactg
     7921 tttacacttt ctacatatgc aacgtgacca aatggtcctt cagaagattg taggatagcg
     7981 cctttagaag gtgtatgatt tactgtgaat ccagcacctt gtgcagcagc agcccaatta
     8041 ttagcatttc cccaagtaga accgatttct ccgccaactt tatcatatac ataccaagta
     8101 cattgtcctg cagtgtataa gttaccagaa tgtgaaattg atgatgtagt tgtcgtagtt
     8161 gtcgtagtcg ttgtagtttg agtcgtgttg tagttatagt tgttgtaatt tgtataattt
     8221 tcagcagcat ctgcatgatg tgcttgacct actaatgctg tgccgattcc tgctgttaac
     8281 gtagttgctg ttactaattt tttcatgaat aaagtcctcc aaagttctat atcttttttt
     8341 ataaataaaa cgtagcgact gttttattct cacatctcga attgatgaca atagttactt
     8401 taacaaaa
//
LOCUS       DAKTIO010000119         8399 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.119, whole genome shotgun sequence.
ACCESSION   DAKTIO010000119 DAKTIO010000000
VERSION     DAKTIO010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8399
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.119"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            12..1535
                     /locus_tag="O0293_001980"
     CDS             12..1535
                     /locus_tag="O0293_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280858.1"
                     /translation="MSKKLKIIIPIIIVLLLIGGIAWGVYAFFANTPKNTYLKSEQQT
                     AKMYKDYFNDRFENEVKFQEKMKDNSFLSSLELSADASDEIVKGLGIPKSVVNASKIK
                     MSYGHDPKKEKSMINLEPTIADSALGKFQLAADKDKHYFESPLFKGKYSVNNSDLLST
                     YSKLTGEDEETAKENGITNQQLNLNTLFNNAQAQQSDYSKIAEKYSELIVDKLDDDNF
                     DKGKKEEIKVNGEKYKVRPVTLTLSRADTKKITLAVLEEAKKDKDLKKLMEEQGATKD
                     FEKDIKKAIDDVKETKKDEFAKIQSKIYTEKHTIVKREITITDKENNKTKIKGTNTLE
                     DDKLKLDYALDFDQDKYTYAEAKYTIKGVSSKEKDNKYNDKYEFGKKTEYDESKIKLD
                     NQEKVDGTKRQDKGKITVALDKYSDENEFTFENNIDSDVKNNTQKSTLNIGIKYAEEP
                     INFILKSSTKLKADIDFDDSGAKDFNSLSSKDREKLEKEIEKNGGKMFESILKKASK"
     gene            1603..2769
                     /locus_tag="O0293_001981"
     CDS             1603..2769
                     /locus_tag="O0293_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0280859.1"
                     /translation="MKTIHLFRIYHSFLLKKWYLIIYLLFILAALLITLTTIQHVTED
                     DNHFNIGVVDKDQSSETKLILNSIGKGSNLGKNVSIKAYDDKQAHTLLKKHKLQGYFV
                     FDKGMTKAFYKQGELPISVYTYDQQSMKSVVLSQLTDSVYQRLMRSMGGILAFQDLAP
                     KASHSDSINVMTDLLITGLNRSGAFNLEPIHLYDTGSYYAITGFLTTVFIFALSLFTV
                     LKMNQDTVLKARLKMFHFSKERLLIIRTLITWFYTMLWSIVGVVWIVFSIPNIFELYN
                     WPTLAIHLSYYVTFLILWLLLIELLTTGLLNSISKVILAIVILVLSGLTIPTIFLQHI
                     ANGVFNIQPFAVVTNQLLEIILNNYILELHPSFYLSFIALLIINLAVLVWRYRQ"
     gene            2766..3686
                     /locus_tag="O0293_001982"
     CDS             2766..3686
                     /locus_tag="O0293_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0280860.1"
                     /translation="MKPYIQLVVFKQWLQYILLVTTIVIALVLIGIGYRVAHDNFKIP
                     ITIQDLDQTTASKSFVNKIKQSDYVTIKKVDEDESYIEDDVTKKEAILSMQIPKGFSQ
                     KLKENRLKETIQLYGRDDFIGGIAVEIVSSSLYEQQIPNIIYEHLEDMKQHQSIDAIN
                     KSYHKHTPESKIKFVSLTKQAQHSISISLIFAVILFVSAVQVVLHYRLNQQAALQRLS
                     QYHLSRFKLYSTYVMTHTILLLLVLLAVSLYLSQPLSLIFYLKSLLLILIYEIGIVFI
                     LFHIQTISHRLFMTFIYALAMGIVYLIIFM"
     gene            3700..4359
                     /locus_tag="O0293_001983"
     CDS             3700..4359
                     /locus_tag="O0293_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0280861.1"
                     /translation="MIEINNLSKRYRNKQIFNHLTMSFDSNRLTVLLGDNGAGKSTLL
                     RMIAGIEKANDGTINYFGEKWNQRQIQNHIGYVPQDIALFEHMTVAENIKFFKSLCKN
                     PINDTTINEYLQQLNFDDTSAKVSTLSGGNKRKINILVGLLGQPRILILDEPTVGIDL
                     KSRHDIHQLLNIMKSKCLIILTTHHLDEVEALADDIKLIGQDPFYQHVLEDKQWTYTY
                     Y"
     gene            complement(4411..5361)
                     /gene="lytM"
                     /locus_tag="O0293_001984"
     CDS             complement(4411..5361)
                     /gene="lytM"
                     /locus_tag="O0293_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-glycine endopeptidase LytM"
                     /protein_id="HCY0280862.1"
                     /translation="MKKLTAAAIATMGFATFTMAHQADAAETTNTQQAHTQMSTQSQD
                     VSYGTYYTIDSNGDYHHTPDGNWNQAMFDNKEYSYTFVDAQGHTHYFYNCYPKNANAN
                     GSGQTYVNPATAGDNNDYTASQSQQHINQYGYQSNVGPDASYYSHSNNNQAYNSHDGN
                     GKVNYPNGTSNQNGGSASKATASGHAKDASWLTSRKQLQPYGQYHGGGAHYGVDYAMP
                     ENSPVYSLTDGTVVQAGWSNYGGGNQVTIKEANSNNYQWYMHNNRLTVSAGDKVKAGD
                     QIAYSGSTGNSTAPHVHFQRMSGGIGNQYAVDPTSYLQSR"
     gene            complement(5687..6679)
                     /locus_tag="O0293_001985"
     CDS             complement(5687..6679)
                     /locus_tag="O0293_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HCY0280863.1"
                     /translation="MCTGFTIQTLNNQVLLGRTMDYDYPLDGSPAVTPRNYRWKSRTG
                     TTGQTQYGFIGTGTDMEGFIYGDGVNEHGVAISTQYFRGYSSYGSTHKADAMNITQNE
                     IVTWILGYTTSIEDMKQQASQIHVVAVYLNDIGEVPPLHYHVSDATGHTVEVSFKEGE
                     VVIKDNPIGVLTNHPDLNWHYSNLRQYINISPYPATANLLEGVTIEPLGNEAGTFGLP
                     GGFTSTERFVRMAFMKANIAQNNDKEMDLMNAFYLLDAVNIPIGIVRPHDADNHYTMY
                     QTVINLTTRTLYIKYYGSNELVALKLTDDLINRKDMTIFKPEKHITIRKLNDNQ"
     gene            6913..8289
                     /locus_tag="O0293_001986"
     CDS             6913..8289
                     /locus_tag="O0293_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0280864.1"
                     /translation="MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTS
                     PAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTYMGLIISMFASLLLIISDITALL
                     IIGRILQGLSAAILLPSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIAS
                     NFGWQTNFIISIVIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLT
                     QGFEQGWFSTFSIICLSIFIITTLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVL
                     NSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIRLGEKATLRFGGKLMLII
                     GPLFPVIGITIISMTQLLASQYVIAVIIGFVICAIGNGLVATPGLTIAIFSMPNEKVG
                     LATGLYKMSGTLGGAFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYM
                     IIPKTVKS"
BASE COUNT         2821 a         1433 c         1356 g         2789 t
ORIGIN      
        1 gaggaagcaa aatgtctaaa aagttaaaaa ttataattcc tattattatt gtcttactat
       61 taataggtgg aatcgcatgg ggagtttatg cattttttgc taatacaccg aaaaatacat
      121 acctaaaaag tgaacaacaa acagcaaaaa tgtataaaga ttattttaat gaccgttttg
      181 aaaatgaagt gaagttccaa gaaaagatga aagataattc gtttttatct tcattagaat
      241 taagcgcaga tgcatcagat gaaattgtta aagggcttgg cattcctaaa tctgttgtta
      301 atgcatcgaa aattaaaatg tcatatggac atgatcctaa aaaagagaaa tcaatgatta
      361 atcttgaacc aacaatcgca gactctgcat tagggaaatt ccagttagct gcagataaag
      421 ataagcatta tttcgaatca ccattattta aagggaaata tagtgttaat aattctgatt
      481 tattatcaac ttattcaaaa cttacaggtg aagatgaaga aacagcaaaa gaaaatggta
      541 ttacaaacca acaactaaat ttaaatactc tttttaataa tgctcaagca caacaaagtg
      601 actacagcaa aattgccgaa aaatattccg aacttattgt cgacaaatta gatgacgata
      661 attttgataa aggtaaaaaa gaagaaatta aggttaatgg tgaaaagtac aaagttagac
      721 ctgtcacgtt aacacttagc agagctgaca ctaaaaaaat tacattagct gtattagaag
      781 aagctaaaaa ggataaagac cttaaaaaat taatggaaga acaaggtgct acaaaagact
      841 ttgaaaaaga catcaaaaaa gcaattgacg atgtcaaaga aactaaaaag gatgaatttg
      901 ctaaaattca atctaaaatt tataccgaaa aacatacgat tgtaaaacga gaaattacta
      961 ttacagacaa agaaaataat aaaactaaaa tcaaaggtac taatacttta gaagacgata
     1021 agttaaaact agattacgca cttgatttcg atcaagataa atacacgtat gctgaagcga
     1081 aatatacaat taaaggcgta tcttctaaag aaaaagataa taaatataat gataaatacg
     1141 aatttggtaa aaagacagaa tatgatgaat caaaaatcaa attagataac caagaaaaag
     1201 tagatggcac aaaacgtcaa gataaaggta aaatcactgt cgcattagat aaatatagcg
     1261 acgaaaatga attcactttt gaaaataata tagattctga cgtaaaaaat aacactcaga
     1321 aatctacgtt aaatatcggc atcaaatatg ctgaagaacc aattaatttc attttaaaat
     1381 ctagcacaaa attgaaagca gatattgatt ttgatgatag tggtgcaaaa gatttcaata
     1441 gtctatcttc aaaagaccgt gaaaaacttg aaaaagaaat cgaaaaaaat ggcggcaaaa
     1501 tgtttgaatc aattttaaaa aaggcatcta aataactaaa atataaaaat aagattggaa
     1561 ggtcatcttg cctttcaatc ttatcattgg aggagtggtt aaatgaaaac aatacatttg
     1621 tttcgcatct accactcttt tttattgaaa aagtggtatt tgattatcta tttactattt
     1681 atattagcgg cactccttat tacattaacg acaatccaac atgtaacaga agatgacaat
     1741 cattttaata taggtgtcgt agataaagat caatcaagtg aaacgaaatt aatcttaaac
     1801 tctattggta aagggagtaa cctaggaaaa aacgtgagca ttaaagcata tgatgataag
     1861 caagcacata ctttgttaaa aaaacataaa cttcaaggct attttgtttt tgataaaggt
     1921 atgaccaagg cattttataa acaaggcgaa ctaccaattt cagtatatac atatgatcaa
     1981 caatccatga aaagtgtcgt gctatctcag ctaacagatt ctgtttacca acgtcttatg
     2041 cgatcaatgg gtggcatctt agcttttcaa gacttagcac cgaaagcatc acattctgac
     2101 agtatcaatg ttatgactga tttgctgatt acaggattaa accgttcagg tgcatttaac
     2161 ttagaaccga ttcatttata cgatacgggc agttattatg caattacagg atttttaaca
     2221 acggtattca tctttgcact atctttattt acagttttga aaatgaatca agatactgta
     2281 ttgaaagcgc gattgaaaat gtttcatttt tctaaagagc gtttattaat cattcgtacg
     2341 ttgattacat ggttttatac tatgttatgg agtatcgttg gtgtagtttg gattgtgttc
     2401 agtattccga atatctttga attatataat tggccaacgt tagccattca tttaagctat
     2461 tatgttacct ttttaatact gtggttatta ctcatcgaac ttttaacaac aggcttgttg
     2521 aatagtatta gtaaagtcat attagctatt gtcatcctgg tgttatctgg tttaacaata
     2581 cctacgattt ttttacaaca tatagcaaat ggtgttttca atattcaacc gttcgcagtt
     2641 gtaacaaatc aattgttaga aattatttta aacaactaca ttttagaact gcaccctagt
     2701 ttctatctta gcttcatcgc acttttgata attaatttag ctgtattggt ttggaggtat
     2761 cgtcaatgaa gccttatata caacttgttg tgttcaagca atggttacaa tacatcttgc
     2821 tcgtaacaac cattgtcatc gcactcgtac ttattggtat cggttaccgt gtagcacatg
     2881 acaacttcaa aataccgatt accattcaag atttagacca aaccactgca tcaaaatcat
     2941 tcgtcaataa aattaaacaa tctgactatg taactattaa aaaagtcgat gaagatgaaa
     3001 gctatattga agatgatgtt actaaaaagg aagctatttt aagtatgcaa attcctaaag
     3061 gtttctctca aaaattaaaa gagaaccgtt taaaagaaac gatacagtta tatggtagag
     3121 atgactttat aggtggtatt gctgtagaaa ttgttagtag ttcattatat gagcagcaaa
     3181 ttcctaacat tatttatgaa caccttgagg atatgaaaca gcatcaatcc atcgatgcta
     3241 tcaacaagtc ctatcataaa catacacctg aatctaaaat caaatttgtg tcgcttacta
     3301 aacaagcaca acactctatt tcaattagct taatctttgc ggtgatttta tttgttagcg
     3361 ctgttcaagt agtacttcat tatcgtttaa accaacaagc agcattgcaa cgattatcac
     3421 aatatcattt aagccgtttc aaactatata gtacttatgt aatgacacat acgattttgt
     3481 tattgttggt actattggca gttagtctat atttgtctca accactcagc ttaatatttt
     3541 acttaaaatc actgttactt atattgattt atgagatagg tatcgtcttt atcttattcc
     3601 atattcaaac aataagtcat cgattattca tgacatttat atatgcactt gctatgggta
     3661 tcgtatactt gattattttc atgtaaagga gcgtaactga tgatagaaat taataacctt
     3721 tcaaagcgtt accgtaacaa acagattttc aatcatttaa ctatgtcctt tgatagtaat
     3781 cgtttaaccg tattacttgg tgataatggt gctggaaaat caacattact tcgtatgatt
     3841 gctggtattg aaaaagctaa tgatggaact atcaactatt tcggcgaaaa atggaatcaa
     3901 agacaaatac aaaatcacat cggttatgtg ccacaagaca ttgcgttatt tgaacacatg
     3961 acagtggctg aaaacattaa attttttaaa tcactttgta aaaatccaat taacgataca
     4021 actatcaacg aatatttaca gcaattaaac tttgatgata cgtctgccaa agtatctaca
     4081 ttgtccggtg ggaataaacg taaaattaat atattagtag gtttactagg tcaacctcga
     4141 attctcattt tagatgaacc gacagttggt attgatttaa aatctagaca tgacatccac
     4201 caactactta acatcatgaa atctaaatgt ttaattatat taactaccca tcatttagat
     4261 gaagttgaag cacttgcaga tgatatcaag ttaattggcc aagatccttt ttatcaacat
     4321 gttttagagg acaaacaatg gacttatacc tattattaaa acgaaaaaat cccaagctgc
     4381 gtatgatatc gcaacttggg attttctgta ttatctactt tgcaagtatg acgttgggtc
     4441 tactgcatat tgattaccga tgccaccaga catacgttgg aagtgtacgt gaggcgctgt
     4501 tgaattaccc gtactacctg aatatgcaat ttggtcacca gctttgactt tatcaccagc
     4561 tgaaacagtt aaacgattat tatgcatata ccattggtag ttattactgt tcgcttcttt
     4621 aatcgttact tgattgccgc caccatagtt actccaacct gcttgtacta ctgtaccatc
     4681 agttaatgag taaactggtg aattttcagg cattgcatag tcgacaccgt aatgcgcacc
     4741 accaccgtga tattgtccat atggttgtag ttgtttacga cttgttaacc agcttgcgtc
     4801 tttcgcatga ccactagctg tcgctttact tgctgatcca ccattttgat tagatgtgcc
     4861 attaggataa ttgacctttc cattaccatc atggctgtta tacgcttggt tgttgttact
     4921 atgtgaataa tagctcgcgt ctggacctac atttgattga taaccatatt gattaatatg
     4981 ctgttggctt tgactcgctg tgtagtcatt gttatctcct gctgttgctg gattcacata
     5041 tgtttggccg cttccattgg catttgcatt ttttggataa cagttataaa aataatgcgt
     5101 atgtccttga gcatctacga atgtatagct atattcttta ttatcaaaca ttgcttgatt
     5161 ccagttacca tcaggtgtgt gatgataatc cccattagaa tcaattgtat aataagtacc
     5221 ataagatacg tcttgtgatt gtgttgacat ttgtgtatgt gcttgttggg tgtttgtcgt
     5281 ttctgctgca tctgcttgat gcgccattgt aaatgtagcg aagcccatcg ttgcaatcgc
     5341 tgctgctgtt aattttttca tgtataaaac atcctccatt aaagttaaag ttagttttca
     5401 attaaactgt actgcacata ctaaaagaat tagacaactg agtaaaggat ttaattctca
     5461 ttttccaact atttaatatt cccgaaatgt tttactaaac tcattacatt gtcattacaa
     5521 aatagccata cattgatatt aaaatgacat ctctcactgc attcgtttaa ccttttataa
     5581 attttcaaaa ttaacaacta atcgttcgtc catgttcgcg attcaacgct aatgcatgat
     5641 agtaatcatc catcaaatca tatcaaccaa attccattat caatcgctat tgattgtcat
     5701 tcaactttct aatagtgata tgcttctcag gcttaaaaat cgtcatatct tttctattaa
     5761 ttaaatcatc tgtgagcttt aatgctacta attcattgct gccataatac ttaatatata
     5821 acgttcttgt agttaaattt attacggtct gatacatcgt atagtgattg tcagcatcat
     5881 gcggacgtac aattccaatc ggtatattta ccgcatctaa taaataaaat gcattcatta
     5941 aatccatttc tttatcattg ttttgagcaa tgtttgcttt cataaatgcc attctcacaa
     6001 agcgctcagt tgaagtaaat ccacctggca atccaaatgt acctgcttca ttgcctaaag
     6061 gttcaatcgt tacaccttcc aataaatttg ctgttgctgg ataaggagaa atattgatat
     6121 attgtcttaa attactataa tgccaattta agtctggatg atttgttaag acaccaatag
     6181 gattatcttt tataaccact tcaccctctt taaatgaaac ttcgactgta tgtccagttg
     6241 catcggaaac atgataatgc aatggcggaa cttcaccgat gtcatttaaa tatacagcta
     6301 caacatgtat ttgggatgct tgttgtttca tatcttcaat gcttgttgta tatcccaaaa
     6361 tccatgtcac aatttcattt tgcgtaatat tcatcgcgtc cgctttgtgt gttgatccat
     6421 atgaactata acctcggaaa tattgtgttg aaatggcaac gccatgttca ttaacaccat
     6481 caccataaat aaaaccttcc atatctgttc ctgtgccaat aaagccatat tgcgtttggc
     6541 ctgtcgtgcc agtgcgagat ttccaacgat aatttctagg cgtcactgct ggcgaaccat
     6601 ctaatggata atcataatcc atcgtgcgtc caagaagtac ttgattattt aaagtttgta
     6661 ttgtgaatcc tgtgcacatt gttctcactc ctctgtacct tcatttactt taatcacttt
     6721 caaataaagc tgtttcactt aaacatacta taaaaaatca attatacaag caattaattg
     6781 atattcattc tcaataactg tggtatgata tgtaaggaaa tcatgactta tgtgtgagtg
     6841 aacgatcatc tatacatccg ttcacttcat ctcatgactt tctatattta atttttacaa
     6901 ggagtgacat ctgtgaataa cacacaatct tcaccacgca gtaatattat tattgcgatt
     6961 atgttgtctg cattaacata ttggttgttt gcacaatcat ttattaatat aggacctctc
     7021 gttggtcaaa cgtatcaaac ctctcctgcc gtgttaaatt tatctattag tttaacttcc
     7081 ttcgccacag gtatcttcat ggtggctgca ggtgatattg ctgataaaat aggacaactg
     7141 agaatgacat acatgggtct cataatcagt atgtttgcat ctcttctatt aattatatcg
     7201 gacatcactg cactgctcat catcggtaga attttacaag gtctatcagc agctatcttg
     7261 ttaccttcaa cagttggcgt gttaaataat caatttaaag gagaacattt aagacgagcg
     7321 attagttatc taatgattag tactgttggt ggcatcggcc tagctggtgt tatcggcggt
     7381 ttaattgcct caaatttcgg atggcaaacg aatttcatca ttagtatagt cattgctttc
     7441 attgccatat tgcttctaaa aggcacacct gaaaaagtaa gtcaacatag ccaccgtcat
     7501 ccattcgatt acaaaggtat gtcgattttc gctgttatga ttggtagctt tacattattg
     7561 ttaacacaag gattcgaaca aggttggttt agtacatttt caatcatttg tctgagcatt
     7621 tttatcatca ctacgttgat attcatcatc atcgaacgtc gacatgaagt accttttatt
     7681 gatttctcag tattacgcaa ccgtccgttc attggtgcat ttttaaataa ctttgtttta
     7741 aatagcggtc taggcgtaac agtggtcttt ttcatatatg ctcaaacaca ccttggttta
     7801 tcagctgcgc aatctggact tgttacattg ccatatgcca ttgtggcagt tgcgatgatt
     7861 cgtttaggtg aaaaagcaac attacgtttc ggtggcaaat tgatgctcat cattggtccg
     7921 ttgttccctg tcatcggcat cactattatt agcatgactc aattattggc atcacaatat
     7981 gtcattgcag ttatcattgg tttcgtcata tgtgcgatag gtaatggttt agtcgcaaca
     8041 cctggactta cgattgcaat tttcagtatg cctaatgaaa aagttggttt agctacagga
     8101 ttatataaaa tgagtggtac attaggtggc gcctttggta tagcactaag tactacagtt
     8161 ttcagtatgt tacaactaaa ctatgcacca agtgtagctg caaccgtaac atttatagtc
     8221 agcattgtat tgatgatcct tggctcattg tctgcataca tgatcattcc aaaaacagtt
     8281 aaatcttaaa tataatagaa gaattatgtt tcgaaatatc tttatcactt taaaatgata
     8341 tacaagaaat ccaagaaaaa taagcgaact gaataaataa agattcaatt aacgcatca
//
LOCUS       DAKTIO010000120         8302 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.120, whole genome shotgun sequence.
ACCESSION   DAKTIO010000120 DAKTIO010000000
VERSION     DAKTIO010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8302
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.120"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..625
                     /locus_tag="O0293_001987"
     CDS             <1..625
                     /locus_tag="O0293_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCY0280865.1"
                     /translation="LSDMERLIRLPGKFKYFEHNVAAHSFKVTKIAQYLATVEEYHGR
                     KINWKSLYEKALNHDFAEVFTGDIKTPVKYASSELKKLFSQVEEEMVETFIEEEIPLQ
                     YRDVYKQRLQEGKDDSLEGQILSVADKIDLLYETFGEIQKRNPEELFFEIYEMSLETI
                     IQFDHLASVQDFINNIIPEMLTENFIPRTELRETTMNILNKRKEENE"
     gene            622..858
                     /locus_tag="O0293_001988"
     CDS             622..858
                     /locus_tag="O0293_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbA family protein"
                     /protein_id="HCY0280866.1"
                     /translation="MIWYFSAAFFPCVLVVLFSVITRSKWVGTILTLILIGASIYKEY
                     FHNEWIIFIDVVSLLAGYLIIDQLEFHKHQDEDR"
     gene            1127..3112
                     /gene="uvrB"
                     /locus_tag="O0293_001989"
     CDS             1127..3112
                     /gene="uvrB"
                     /locus_tag="O0293_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002473941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCY0280867.1"
                     /translation="MVEHYPFKIHSDFEPQGDQPQAIKEIVEGIKAGKRHQTLLGATG
                     TGKTFTMSNVIKEVGKPTLIIAHNKTLAGQLYSEFKEFFPENRVEYFVSYYDYYQPEA
                     YVPSTDTFIEKDASINDEIDQLRHSATSALFERDDVIIIASVSCIYGLGNPEEYKDLV
                     VSVRVGMEMDRSELLRKLVDVQYTRNDIDFQRGTFRVRGDVVEIFPASKEELCIRVEF
                     FGDEIDRIREVNYLTGEVLKEREHFAIFPASHFVTREEKLKVAIERIEKELEERLKEL
                     RDENKLLEAQRLEQRTNYDLEMMREMGFCSGIENYSVHLTLRPLGSTPYTLLDYFGDD
                     WLVMIDESHVTLPQVRGMYNGDRARKQVLVDHGFRLPSALDNRPLKFEEFEEKTKQLV
                     YVSATPGPYEIEHTDKMVEQIIRPTGLLDPKIEVRPTENQIDDLLSEIQTRVERNERV
                     LVTTLTKKMSEDLTTYMKEAGIKVNYLHSEIKTLERIEIIRDLRMGTYDVIVGINLLR
                     EGIDIPEVSLVVILDADKEGFLRSNRSLIQTIGRAARNDKGEVIMYADKMTDSMKYAI
                     DETQRRREIQMKHNEKHGITPKTINKKIHDLISATVENDENNDKAQTVIPKKMTKKER
                     QKTIDNIEKEMKQAAKDLDFEKATELRDMLFELKAEG"
     gene            3120..5966
                     /gene="uvrA"
                     /locus_tag="O0293_001990"
     CDS             3120..5966
                     /gene="uvrA"
                     /locus_tag="O0293_001990"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCY0280868.1"
                     /translation="MKEPSIVVKGARAHNLKDIDIELPKNKLIVMTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMDKPDVDTIEGLSPAISIDQKTTSKNPRSTVAT
                     VTEIYDYIRLLYARVGKPYCPNHNIEIESQTVQQMVDRIMELEARTKIQLLAPVIAHR
                     KGSHEKLIEDIGKKGYVRLRIDGEIVDVNDVPTLDKNKNHTIEVVVDRLVVKDGIETR
                     LADSIETALELSEGQLTVDVIDGEDLKFSESHACPICGFSIGELEPRMFSFNSPFGAC
                     PTCDGLGQKLTVDVDLVVPDKDKTLNEGAIEPWIPTSSDFYPTLLKRVCEVYKINMDK
                     PFKKLTERQRDILLYGSGDKEIEFTFTQRQGGTRKRTMVFEGVVPNISRRFHESPSEY
                     TREMMSKYMTELPCETCHGKRLSREALSVYVGGLNIGEVVEYSISQALNYYKNIDLSE
                     HDQAIANQILKEIISRLTFLNNVGLEYLTLNRASGTLSGGEAQRIRLATQIGSRLTGV
                     LYVLDEPSIGLHQRDNDRLINTLKEMRDLGNTLIVVEHDDDTMRAADYLVDIGPGAGE
                     HGGQIVSSGTPQKVMKDKKSLTGQYLSGKKRIEVPEYRRPASDRKISIRGARSNNLKG
                     VDVDIPLSIMTVVTGVSGSGKSSLVNEVLYKSLAQKINKSKVKPGLYDKIEGIDQLDK
                     IIDIDQSPIGRTPRSNPATYTGVFDDIRDVFAQTNEAKIRGYQKGRFSFNVKGGRCEA
                     CKGDGIIKIEMHFLPDVYVPCEVCDGKRYNRETLEVTYKGKNIADILEMTVEEATQFF
                     ENIPKIKRKLQTLVDVGLGYVTLGQQATTLSGGEAQRVKLASELHKRSTGKSIYILDE
                     PTTGLHVDDISRLLKVLNRLVENGDTVVIIEHNLDVIKTADYIIDLGPEGGSGGGTIV
                     ATGTPEDIAQTKSSYTGKYLKEVLERDKQNTEDK"
     gene            6628..7560
                     /locus_tag="O0293_001991"
     CDS             6628..7560
                     /locus_tag="O0293_001991"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HCY0280869.1"
                     /translation="MLTTEKLVETLKLDLIAGEEGLSKPIKNADISRPGLEMAGYFSH
                     YASDRIQLLGTTELSFYNLLPDKDRAGRMRKLCRPETPAIIVTRGLQPPEELVEAAKE
                     LNTPLIVAKDATTSLMSRLTTFLEHALAKTTSLHGVLVDVYGVGVLITGDSGIGKSET
                     ALELVKRGHRLVADDNVEIRQINKDELIGKPPKLIEHLLEIRGLGIINVMTLFGAGSI
                     LTEKRIRLNINLENWNKQKLYDRVGLNEETLSILDTEITKKTIPVRPGRNVAVIIEVA
                     AMNYRLNIMGINTAEEFSERLNEEIIKNSHKSEE"
     gene            7566..>8302
                     /locus_tag="O0293_001992"
     CDS             7566..>8302
                     /locus_tag="O0293_001992"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCY0280870.1"
                     /translation="MGIVFNYIDPVAFNLGPLSVRWYGIIIAVGILLGYFVAQRALVK
                     AGLHKDTLVDIIFYSALFGFIAARIYFVIFQWPYYAENPSEIIKIWHGGIAIHGGLIG
                     GFIAGVIVCKVKNLNPFQIGDIVAPSIILAQGIGRWGNFMNHEAHGGSVSRAFLEQLH
                     LPNFIIENMYINGQYYHPTFLYESIWDVAGFIILVNIRKHLKLGETFFLYLTWYSIGR
                     FFIEGLRTDSLMLTSNIRVAQLVSILL"
BASE COUNT         2987 a         1137 c         1674 g         2504 t
ORIGIN      
        1 attatcagat atggaaagac ttataagatt acctggaaaa tttaaatatt ttgaacacaa
       61 tgttgcagca cactccttta aagtaactaa aattgcacaa tatctagcaa cagttgaaga
      121 atatcatgga cgaaagatta attggaaaag cttatatgaa aaagcattaa atcatgattt
      181 cgccgaagtg tttactggtg atataaaaac acctgttaaa tatgcgagta gtgaattaaa
      241 aaaattattt tcgcaagttg aagaagaaat ggtagagacc tttattgaag aagaaattcc
      301 attacaatat agagatgttt ataagcaacg actgcaagaa ggtaaagatg attcattaga
      361 aggccaaata ctttcagttg ctgataaaat tgatttgctt tatgaaacat ttggagaaat
      421 acaaaaacgt aatcccgaag aattattttt cgaaatttat gaaatgagtc tagaaacaat
      481 tattcaattt gaccatttag catctgtaca agattttatt aataatatca ttccagaaat
      541 gttgactgaa aactttatac ctagaacaga attaagagaa acaaccatga acattttaaa
      601 taaaagaaaa gaggaaaatg aatgatatgg tattttagcg cagcattctt tccatgtgtc
      661 ttggtagtat tatttagtgt aataacaaga agtaaatggg tcggtactat tctgacatta
      721 attttaattg gtgcctcaat ctataaagag tatttccata acgagtggat tatttttatt
      781 gatgtagtgt cattattagc tggttattta attatagatc aactcgaatt tcataaacat
      841 caagatgaag atcgctaaga ttaactttaa aataatgttt caaacaaatt tgttgaaaca
      901 aaatgatgat taatataatg tgtatttaca tactaaaaat aacaagataa acgatttgat
      961 ttaaggcaag catagttagc acaactatgt ttgtttttct ttgttcgaca tttttacgaa
     1021 caaacgtttg ctttttgtgt gactactttg ctaaaatatg taatgagaaa gcaaagagtt
     1081 gagcggaaat ataaataata cgttgaaaga ggaggcatat gtgacaatgg ttgaacatta
     1141 tccttttaaa atacattctg attttgagcc tcaaggtgac caaccgcaag caattaaaga
     1201 aatcgtggaa ggtattaaag cggggaaaag acatcaaact ttattaggtg ctactggcac
     1261 agggaaaaca tttacgatga gtaatgttat taaagaagtt gggaaaccaa cgttaattat
     1321 cgcgcataac aaaacattag caggacaatt atatagtgag tttaaagaat tttttcctga
     1381 aaacagggtg gaatactttg taagttacta tgattattat caaccagagg catacgtacc
     1441 gtctactgac acttttattg aaaaagatgc ctcaatcaat gatgaaattg atcaactacg
     1501 acattctgct acaagtgcat tatttgaacg cgatgatgta attattattg ctagtgtaag
     1561 ttgtatatat ggtttaggta atcctgaaga atataaagat ttagtagtaa gtgttcgagt
     1621 tggtatggaa atggatagaa gtgaattact tagaaaactt gtagatgtgc aatatacacg
     1681 aaatgacatc gatttccaac gaggaacgtt tcgagtgcgt ggtgatgtag tggaaatatt
     1741 cccagcctct aaagaagaac tttgtataag ggttgagttt ttcggcgatg agattgaccg
     1801 tatccgagaa gttaactacc taacaggtga agtgttgaaa gaaagagaac attttgcgat
     1861 attcccagct tctcacttcg taacacgtga agaaaagttg aaagttgcga ttgaacgtat
     1921 tgaaaaagaa ttggaagaac gattgaaaga attacgagat gagaataaat tactagaagc
     1981 gcaaaggtta gaacagcgta ccaactatga tttagaaatg atgcgagaga tgggattctg
     2041 ttcaggaatt gaaaactatt ccgtacattt aactttgcga ccactgggtt cgacaccata
     2101 tactttattg gattactttg gcgatgattg gttagtaatg attgatgaat cacatgtgac
     2161 attaccgcaa gttcgaggca tgtataacgg agacagagcg cgtaaacaag ttttggtgga
     2221 tcatgggttt agattaccga gtgcattaga taaccgtcca cttaaatttg aagaatttga
     2281 agaaaagaca aaacaacttg tgtatgtatc tgcaacgcct ggaccatacg aaattgaaca
     2341 tacggataag atggttgaac aaattattcg tcctactggt ttactggatc ctaagattga
     2401 ggttagacct actgaaaatc aaattgacga tttattaagt gaaattcaaa caagagttga
     2461 gcgtaatgaa cgcgtacttg ttacaacgct cactaaaaag atgagtgaag atttaaccac
     2521 atacatgaaa gaagcgggta ttaaagttaa ttatctgcat tcagaaatca agacattaga
     2581 acgaattgaa ataattagag acttacgaat gggtacatat gatgttatcg taggtattaa
     2641 tttattaaga gagggtattg atataccaga agtttctcta gttgtcatat tagatgcaga
     2701 taaagaaggg tttttacgtt ctaaccgctc attaattcaa acaataggta gagctgcgcg
     2761 taacgataaa ggtgaagtca ttatgtatgc cgataaaatg actgattcga tgaagtatgc
     2821 aattgatgag acacaacgtc gtcgagaaat acagatgaaa cataatgaaa aacatggtat
     2881 tacacctaaa acaattaata aaaaaataca tgatttaatt agtgctactg ttgaaaatga
     2941 cgaaaataat gacaaagcac aaactgtgat acctaagaag atgacgaaaa aagaacgtca
     3001 aaagacaatc gacaatatag aaaaagaaat gaaacaagca gcgaaagatt tagatttcga
     3061 gaaagctaca gaattaagag atatgttatt tgaattaaaa gcagaagggt gacaagtaaa
     3121 tgaaagaacc atccatagta gtaaaaggtg ctcgtgcgca taacttgaaa gatattgata
     3181 tcgaactacc taaaaataaa ttaattgtta tgacaggttt atctgggtca ggtaaatcgt
     3241 cattagcatt cgatactata tatgctgaag gacaacgacg ttatgttgaa tcattaagtg
     3301 cctatgcgcg tcaattttta ggccaaatgg acaaaccaga tgttgataca attgaaggat
     3361 tatcgccagc aatttcaata gatcaaaaaa caacaagtaa aaatccaaga tcaactgtag
     3421 caacagtaac agaaatatat gattatatac gtttgttata tgcacgtgtt ggtaaacctt
     3481 actgtccaaa tcacaatata gaaattgaat cgcaaacagt acaacaaatg gttgaccgca
     3541 ttatggaatt agaggcacgt acaaagattc aattattagc acctgtcatc gctcatcgta
     3601 aaggtagtca tgaaaagcta atcgaagata ttggtaaaaa aggttatgta cgtttaagaa
     3661 tcgatggcga aattgttgat gtaaatgatg tacctacttt agataagaac aagaatcata
     3721 caatagaagt tgttgtagac cgattagttg ttaaagatgg aattgaaaca cgactagctg
     3781 actctataga aactgcctta gagctttcag aaggacaatt aacagtcgat gtcattgacg
     3841 gggaagacct taagttttca gaaagccatg cttgtcctat atgtggattt tcaatcggag
     3901 agttagaacc aagaatgttt agctttaaca gtccttttgg tgcttgtccg acatgtgatg
     3961 gcttaggcca aaagttaaca gtcgatgtag acttggttgt tcccgacaaa gataagacgc
     4021 taaacgaagg tgcaatagaa ccttggatac cgacgagttc tgatttttat ccaacattgt
     4081 taaaacgtgt ttgtgaagtt tataaaatca atatggataa accttttaaa aagttaacag
     4141 aacgtcaacg tgatatttta ttgtatggtt ctggtgacaa agaaattgaa tttacattta
     4201 cacaacgtca aggtggtact agaaaacgaa caatggtttt cgagggtgta gttcctaata
     4261 taagtagacg attccatgaa tctccttcag aatatacacg tgaaatgatg agtaaatata
     4321 tgactgaact accttgcgaa acttgtcatg gaaagcgatt gagtcgtgaa gcgttatctg
     4381 tttatgtagg tggtttaaat attggtgaag tagtcgaata ttcaatcagt caagcgctga
     4441 actattataa aaacattgat ttgtcagaac atgatcaagc gattgcaaat caaatattga
     4501 aagaaattat ttcccgactc acttttttaa ataatgtggg acttgaatat ttaacgttaa
     4561 acagagcttc aggtacactt tcaggtggtg aagcacaacg tattcgatta gcaacgcaaa
     4621 ttgggtcgcg tttgactggt gtcttatatg tattagatga gccatcaatt ggactgcatc
     4681 aaagagataa tgatcgatta attaatacac ttaaagaaat gagagattta ggaaatactt
     4741 taattgtagt tgaacacgat gatgatacaa tgcgtgcggc tgattactta gtggatatag
     4801 gtcctggtgc tggtgaacat ggagggcaga ttgtgtctag tggtactcct caaaaggtaa
     4861 tgaaagataa aaaatcatta acaggacaat acttgagtgg taagaaacgt attgaagtac
     4921 ctgaatatcg cagaccggct tcagatcgta aaatttctat acgtggagct agaagcaaca
     4981 atcttaaagg ggttgatgtg gacataccac tatcaatcat gacggttgtt acaggtgtat
     5041 caggttctgg taaaagctca ttagtaaatg aagtattata caaatcatta gctcaaaaaa
     5101 ttaataaatc taaagtaaag ccaggattgt acgataagat tgaaggtatt gatcaacttg
     5161 ataaaattat tgatattgat caatcaccaa taggtagaac gccacgctct aatccagcaa
     5221 catatactgg tgtgtttgat gatatacgtg atgtgtttgc gcaaacaaat gaagctaaaa
     5281 ttcgaggata tcaaaaaggg cgttttagtt ttaatgtaaa aggtggacgc tgtgaagctt
     5341 gtaaaggtga cggtattatt aaaattgaaa tgcatttttt acctgatgtt tatgttcctt
     5401 gtgaagtgtg tgatggtaaa cgatataatc gtgagacact agaggttact tacaaaggta
     5461 aaaatattgc tgacatttta gaaatgactg ttgaagaagc aacacaattt tttgaaaata
     5521 ttcctaagat taagcgcaag ttacaaacac tagttgatgt tggtcttgga tacgtcacat
     5581 taggtcaaca agctacaacg ttatcaggtg gtgaggctca acgtgtgaaa cttgcatctg
     5641 aacttcataa acgttcaact ggtaaatcta tttatatcct agatgaaccg acaacagggt
     5701 tacatgttga cgatattagt agattattaa aagtattaaa ccgattagtt gaaaatggtg
     5761 atactgttgt aattattgaa cataacctag atgttatcaa aacagcagac tatattatag
     5821 acttaggtcc tgaaggtggt agtggcggtg gtactattgt tgcgactggc acacccgaag
     5881 atattgctca gacaaagtca tcatatacag gaaagtattt aaaagaagta cttgaacgag
     5941 ataaacaaaa tactgaagat aaataagatt aaaagaagtg aaggatgtta taaatttatc
     6001 cttcgcttct ttttattaat ttagtaatga atagtagaaa gaaaagatgc gtaaaaagaa
     6061 ttatgttaag atagggtcaa tctagagtag ttaaacataa atcgaactgg gagtgggaca
     6121 gaaatgataa agaatcacta atgatttatt atgtagtggt tctttgtcat tagccacagc
     6181 tattgtgtac ttaaaaatag gaatgcatga gtgcaactca tgcataagaa atactaattt
     6241 ctaaagaaaa agtatttctt tatgttgggg ccccgccaac ttgcattgtt tgtagaattt
     6301 cttttcgaaa ttctttatgt tggggccccg ccaacttgca ttgtttgtag aatttctttt
     6361 cgaaattctt tatgttgggg ccccgccaac taattccaat atatcattgt agagcttagg
     6421 tcattgattt ttggctcgga cttttatggc gatatgaacc atgtaaatta agcaagcaat
     6481 aaattaatga ttgatattga cttgtaaaat aataacaata atgaacaatt aatatttatt
     6541 ttagcttttc aatgtagatt ggtgttatat ttttgatatg ataagaagag atgtaagagt
     6601 agggataaat acaattgagg tgaacccatg ttaacgacag aaaaactagt tgaaacatta
     6661 aagttagatt taatcgctgg tgaagaagga ctatcgaagc caattaaaaa tgctgatata
     6721 tcaagaccgg gcttagagat ggcaggttat ttttcacatt atgcgtcaga tagaatacaa
     6781 ctattaggaa caacggaact atcgttttac aatttattac cagataagga tcgcgcaggt
     6841 cgtatgcgta aactatgcag accagaaacg cctgcaatta ttgtgacacg tggattgcag
     6901 ccaccagaag aattagttga agctgcaaaa gaattaaata ccccacttat agttgctaaa
     6961 gatgcgacta caagtttaat gagtcgctta acaacgtttt tagagcatgc acttgcaaag
     7021 acgacatctt tacatggtgt tttagtagat gtttacggtg ttggtgtact aattaccggt
     7081 gattcaggaa taggtaaaag tgagactgcg ttggaattag ttaaacgtgg gcatagatta
     7141 gtagcagatg ataatgtaga aatacgtcaa attaataaag atgaactaat agggaaacca
     7201 ccaaagttaa tagaacatct attagaaata cgtggactag gtattatcaa tgttatgact
     7261 ttatttggcg cgggttcaat attaactgaa aaacgaatta gattaaatat taatttggaa
     7321 aactggaaca agcaaaagtt atatgaccgc gtaggtctta atgaagagac gctaagtatt
     7381 ttagatactg aaatcactaa aaaaacaata cctgtaagac ctggtagaaa tgttgcggta
     7441 attattgagg tcgctgcaat gaactatcga ttaaatatca tgggcattaa cacggccgaa
     7501 gaatttagtg aaagattaaa tgaagaaatt atcaagaaca gtcataagag tgaggagtag
     7561 gttgaatggg tattgtattt aactatatag atcctgtggc atttaactta ggaccactga
     7621 gtgtacgatg gtatggaatt atcattgctg tcggaatatt acttggttac tttgttgcac
     7681 aacgtgcact agttaaagca ggattacata aagatacttt agtagatatt attttttata
     7741 gtgcactatt tggatttatc gcggcacgaa tctattttgt gattttccaa tggccatatt
     7801 acgcggaaaa tccaagtgaa attattaaaa tatggcatgg tggaatagca atacatggtg
     7861 gtttaatagg tggctttatt gctggtgtta ttgtatgtaa agtgaaaaat ttaaacccat
     7921 ttcaaattgg tgatatcgtt gcgccaagta taattttagc gcaaggaatt ggacgctggg
     7981 gtaactttat gaatcacgag gcacatggtg gatcggtgtc acgcgctttt ttagaacaat
     8041 tacatttgcc taattttata atagaaaata tgtatattaa cggccaatat tatcatccaa
     8101 cattcttata tgaatccatt tgggatgtcg ctggatttat tatcttagtt aatattcgta
     8161 aacatttaaa attaggagaa acattctttt tatatttaac ttggtattca attggtcgat
     8221 tctttataga aggattacgt acagatagct taatgctcac aagtaatatt agagttgcac
     8281 aattagtatc aattctttta at
//
LOCUS       DAKTIO010000121         8238 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.121, whole genome shotgun sequence.
ACCESSION   DAKTIO010000121 DAKTIO010000000
VERSION     DAKTIO010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8238
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.121"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..1051)
                     /locus_tag="O0293_001993"
     CDS             complement(<1..1051)
                     /locus_tag="O0293_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HCY0280871.1"
                     /translation="MKEFKEQFGYQLSNFDDMDIKGYANLYQKDIGKDVSMIEQGLKQ
                     LSITETEVLLPEQINKKLLGVLNMNEVNQSSNTWVGTLTKQLVSSENNFNIQELPTAR
                     KEVFKELLVNCELPQSLREVITITDDEHVESIPSLTYIKDKLATNGIELSLNGSSKYF
                     DRREGHIYTEVSDSVVHGSDRTLDDLLKEIFINECVAYETTLLLDKNNSSGLIDKGNQ
                     DLSLYNQGIKEVSNTSIYDGIKQAMKDIPQTFRRNVSIVMNTEHHDKLIKELAQMGLG
                     GLAGDLSNLFNVTNVVVTDDAHDVFVGDFGHAIYAKYEPIMYNKKKQALKGIYQFALN
                     YVFDIKIIPELLRIVN"
     gene            complement(1038..1244)
                     /locus_tag="O0293_001994"
     CDS             complement(1038..1244)
                     /locus_tag="O0293_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280872.1"
                     /translation="MLDHTNESVKRVFKYRDKQELLNKYHELLDKYFLHNISFYEEDK
                     NNERIYGLVVHTKNKLGDEDYERV"
     gene            complement(1469..1813)
                     /locus_tag="O0293_001995"
     CDS             complement(1469..1813)
                     /locus_tag="O0293_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCY0280873.1"
                     /translation="MNRNRMKQIILEYIKNNDSTSFVEIENVFEEQGFKYKGNGAYTS
                     GNHKNIIFWMGWNEEAFNIVADLKRDGLIEMQICPPMYYLIDGKGLRLPIVKNKNIKI
                     DHWLPVTFSLVN"
     gene            complement(2484..4856)
                     /locus_tag="O0293_001996"
     CDS             complement(2484..4856)
                     /locus_tag="O0293_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCY0280874.1"
                     /translation="MAIKKKDRIIGVKELEIPQELKLVPNWVLWRAEWNEKQQNFGKV
                     PYSINGYRASTTNKKTWCDFESVSIEYEVDEQYSGIGFVLSDGNNFVCLDIDNAIDKK
                     GQINSELALKMMQLTYCEKSPSGTGLHCFFKGKLPDNRKKKRTDLDIELYDSARFMTV
                     TGCTIGQSDICDNQEVLNTLVDEYFKENLPANEVVREESNTNIQLSDEDIINIMMKSK
                     QKDKIKDLLQGTYESYFESSSEAVQSLLHYLAFYTGKNKQQMERIFLNYNNLTDKWES
                     KRGNTTWGQLELDKAIKNQKTIYTKSIDEFNVIPQGSKDVKQLLNQLGHEERTKMEEN
                     WIEEGKRGRKPTTISPIKCAYILNEHLTFILFDDEENTKLAMYQFDEGIYTQNTTIIK
                     RVISYLEPKHNSNKADEVIYHLTNMVDIKEKTNSPYLIPVKNGVFNRKTKQLESFTPD
                     YIFTSKIDTSYVRQDIVPEINGWNIDRWIEEIACNDNQVVKLLWQVINDSMNGNYTRK
                     KAIFFVGDGNNGKGTFQELLSNVIGYSNIASLKVNEFDERFKLSVLEGKTAVIGDDVP
                     VGVYVDDSSNFKSVVTGDPVLVEFKNKPLYRATFKCTVIQSTNGMPKFKDKTGGTLRR
                     LLIVPFNANFNGIKENFKIKEDYIKNQQVLEYVLYKAINLDFETFDIPDASKKMLEVF
                     KEDNDPVYGFKVNMFDQWTIRKVPKYIVYAFYKEYCDENGYNALSSNKFYKQFEHYLE
                     NYWKTDAQRRYDNEELAKRIYNFNDNRNYIEPIESGKNYKSYEKVKLKAI"
     gene            complement(4948..5196)
                     /locus_tag="O0293_001997"
     CDS             complement(4948..5196)
                     /locus_tag="O0293_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1474 family protein"
                     /protein_id="HCY0280875.1"
                     /translation="MNWEIKNLMCDLKILKEKFEDLKDNHGSHFEDLYLHEPNHTLNK
                     DDAIKEGFSYHERRIHNDQMFDALEKASSFADQSQDNA"
     gene            complement(5197..5580)
                     /locus_tag="O0293_001998"
     CDS             complement(5197..5580)
                     /locus_tag="O0293_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCY0280876.1"
                     /translation="MEIKQKYQLSKVVKILEVVLYEEDKFQSDKDYHYQDKALYEYAL
                     KLVHNGLFNILAELDFEDEAFLILDEVTMTLSDVMKETQHVYRYSVIDEKGEHKHTTD
                     RKGHVIGMLEWALDYIVGNIEVEEL"
     gene            complement(5582..5785)
                     /locus_tag="O0293_001999"
     CDS             complement(5582..5785)
                     /locus_tag="O0293_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCY0280877.1"
                     /translation="MRKQVIITKTVVGWYNIKDTQHNLMLNIPPKVFEQYFPDVSKDV
                     QVACLEMDLSKITEIKNKKKVGS"
     gene            complement(5752..5925)
                     /locus_tag="O0293_002000"
     CDS             complement(5752..5925)
                     /locus_tag="O0293_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCY0280878.1"
                     /translation="MKMYLTYICLVSLLTILLLAISNMYVAFSVYGMMVTYGFNLTGE
                     ITTCENKLLLQKQ"
     gene            complement(5937..6209)
                     /locus_tag="O0293_002001"
     CDS             complement(5937..6209)
                     /locus_tag="O0293_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCY0280879.1"
                     /translation="MSRTKLHDVPAKENTITEPKQVVVNPLFAKPNALASILGISYSS
                     VNRILKEWEKDSKGVDDLYYSLSSTMIVISIPRFEEYMKARHKKWM"
     gene            complement(6202..6465)
                     /locus_tag="O0293_002002"
     CDS             complement(6202..6465)
                     /locus_tag="O0293_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0280880.1"
                     /translation="MVLNLKRLRAERIACGITQDEMAHKMGWKTRTPYAKRENGIVDI
                     GANEFIKMAKILGYETNNLDIFFTNNVPGKERKNILKGGELNV"
     gene            6658..6990
                     /locus_tag="O0293_002003"
     CDS             6658..6990
                     /locus_tag="O0293_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0280881.1"
                     /translation="MRTNDEIITIIKTSMKEQNMSLSELARRVGVAKSAVSRYLNLTR
                     EFPLNRAEDFAKVLGIKTEYLLGFAEREESTKQDTIAAHLDGDFTEEELIEIRKYAEL
                     VRKAHRNQ"
     gene            7002..7460
                     /locus_tag="O0293_002004"
     CDS             7002..7460
                     /locus_tag="O0293_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCY0280882.1"
                     /translation="MYLYEKMVIENKEIPIDDGKSLGNFEGLYDNGVILINKNLSETR
                     KAEVLYEELAHHKLTYGNILDQSKWINRKFENYARRHGFISAVPLREIVEAYNYGVRN
                     LYELSEYLQLSEKYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI"
     gene            7477..8205
                     /gene="seq"
                     /locus_tag="O0293_002005"
     CDS             7477..8205
                     /gene="seq"
                     /locus_tag="O0293_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type Q"
                     /protein_id="HCY0280883.1"
                     /translation="MNKIFRVLTVSLFFFTFLIKNNLAYADVGVINLRNFYTNYQPET
                     LQGVSSGNFSTSHQLEYIDGKYTLYSQFHNEYEAKRLKDHKVDIFGISYSGLCNTKYM
                     YGGITLANQNLDKPRNIPINLWVNGKQNTISTDEVSTQKKEVTAQEIDIKLRKYLQNE
                     YNIYGFNKTKKGQEYGYQSKFNSGFNKGKITFHLNNETSFTYDLFYTGTGQAESFLKI
                     YNDNKTIDAENFHLDVEISYEKTE"
BASE COUNT         2602 a         1447 c         1077 g         3112 t
ORIGIN      
        1 tattaactat gcgcaataac tctggaatta tttttatatc aaacacatag ttcaatgcaa
       61 attgataaat accttttaat gcttgtttct ttttgttata cataataggt tcgtatttag
      121 catatatggc atgtccaaag tctccaacaa agacatcatg tgcatcatca gtaaccacta
      181 cattagtcac attaaatagg ttacttaaat cacctgcaag tccacctaat cccatctgtg
      241 ccaactcttt gatgagctta tcatgatgtt ctgtgttcat cacaatagat acattacgcc
      301 taaacgtttg tggtatatct ttcatcgctt gcttgatacc atcgtatatt gatgtgttac
      361 ttacttcttt gataccttga ttataaagtg ataagtcttg gttaccttta tcaattaaac
      421 cacttgaatt attcttatct aataacaatg ttgtttcata tgcgacacac tcattaataa
      481 atatctcttt aagtagatca tctaatgttc tatcagagcc atgaactaca ctgtctgata
      541 cttctgtgta gatatgtccc tcacgtctat caaagtattt actactgcca ttaagtgata
      601 gctcaatacc gttagtagct aacttatctt taatgtatgt tagtgatggt atagattcaa
      661 catgttcatc atcagtaatg gtaatcacct cacgtagtga ttgaggtagc tcacaattca
      721 ccaataactc tttaaatact tctttacgtg cagttggtaa ctcttgaata ttaaagttat
      781 tctcactact aactaattgt tttgttaagg tacctaccca tgtattactt gattgattta
      841 cttcattcat atttaacact cctaataatt ttttgttaat ttgctctggt aataaaactt
      901 ctgtttcagt aatacttaat tgctttaacc cctgttcaat catacttaca tctttaccta
      961 tatccttttg atatagattg gcatatcctt taatatccat atcatcaaag ttagatagtt
     1021 gatacccaaa ttgttcttta aactctttca taatcttcat ctcctaattt gtttttagta
     1081 tgaacaacta aaccatatat acgttcgttg tttttatctt cttcatagaa cgaaatgtta
     1141 tgaagaaagt atttgtctaa taattcgtga tacttattta atagttcttg cttatctcta
     1201 tacttaaaca cacgttttac agattcattt gtatgatcta gcacgtcttg cctctctcct
     1261 tttaatctga tagatataca ttttaataag ttcaaaatca gcacgctttt ttgtatagcg
     1321 ccttacaacg cgatttatat atttcgtatt cattaattga acgttcacag gtgtatgttt
     1381 gattatttga tatgcgattc ttttatgatt aataaatata cacactccca tatctatact
     1441 tgatatttta aatgcttatc tgcatgtttt aatttactaa actaaatgtt actggtagcc
     1501 aatgatctat ttttatattt ttattcttta caattggtag ccttagacct ttaccatcaa
     1561 taagatagta cattggcgga caaatttgca tttcaattag cccgtcgcgt tttaaatctg
     1621 ccacaatgtt aaacgcttct tcattccaac ccatccaaaa tataatattc ttatggttgc
     1681 cactggtata tgcgccgtta cctttgtatt taaaaccttg ttcttcaaat acattctcaa
     1741 tctctacaaa tgaagtgctg tcattgttct ttatatattc taagatgatt tgtttcattc
     1801 tatttctatt catgttactc tagtcctcca atctatccag ttactaaagc aaccacgtta
     1861 gatactaaaa accactgaat ttatgttttt caataaccac tgtaaacaca gtgatgtcaa
     1921 tacatacagg ctgttggtta ctgagttact gaaaattcac ggtattttat ataatctttt
     1981 attaccttaa ttctctatta tacatttcta ctaaaaagaa ataatttata gtaactcagt
     2041 aactttttgt caaaaagact aatatgaaag taaaaactgt ggttactgaa ttctagaatt
     2101 ttagtatctt ttagtgactt aaacgttttt tagtaacttt agtatcttag gcaattttgt
     2161 ataactgcaa gtctcttccg attatgtgca actgaaattt acagctatgc aactcgtttt
     2221 aaattttcca gttgcaattt ttttcttact gtcacaaggg gttaatccac ttttgcaact
     2281 atgcaaccga aatcacacaa attagtttat ataatttttg atactatttc ttatttatat
     2341 tttggttatt tacttttcta ctaataagtt atagattttg agttgctcag ttgcataagt
     2401 gccctaaacc cttgatataa ctgtgattat aggtgcaacc cgttttaaaa ttttcggttg
     2461 cattcggttg cattcggttg caactatatt gcttttagct tcaccttttc atacgattta
     2521 tagttttttc cactttcaat aggttcaatg taatttctat tgtcattaaa gttgtatatc
     2581 ctcttagcaa gttcttcatt gtcatatctt cgctgtgcat cagttttcca ataattctct
     2641 aaataatgtt caaattgttt ataaaacttg tttgaactca atgcattata gccattttca
     2701 tcacaatatt ctttataaaa tgcgtataca atatatttcg gcacttttct aatagtccat
     2761 tgatcaaaca tatttacttt aaacccataa actggatcgt tatcttcttt aaatacttca
     2821 agcatttttt tagatgcatc aggaatgtca aaggtttcaa aatctaaatt aattgcttta
     2881 taaagcacat actctagcac ttgctgattt tttatatagt cttctttgat tttaaaattc
     2941 tctttaatgc cattaaaatt ggcattaaac ggtactatca ataaccttct taaagtcccg
     3001 cctgttttgt ctttaaattt aggcattcca ttcgttgatt gaataactgt acacttaaaa
     3061 gtcgctctat ataagggttt atttttaaac tcaaccaaca ctggatcgcc agtaactaca
     3121 cttttaaagt ttgaagaatc atcgacatac acaccaactg gtacatcgtc tccaattact
     3181 gctgtcttgc cctctaacac actcaattta aaacgttcat caaactcatt cacttttaag
     3241 ctagcaatat tgctataacc tattacatta gacaataatt cttgaaacgt ccctttacca
     3301 ttatttccat caccaacaaa gaaaatagcc tttttacgag tgtagttacc attcattgag
     3361 tcattaatta cttgccacaa taatttaaca acctgattat cattacaagc aatctcttct
     3421 atccaccgat ctatattcca gccatttatt tcaggtacta tatcttgcct tacatacgat
     3481 gtatctattt tactggtaaa tatataatca ggtgtaaatg attctagttg cttcgtttta
     3541 cggttaaata caccattttt aactggtatt aagtatggtg agttagtttt ctcttttata
     3601 tctaccatat tggttaaatg ataaataact tcatcagctt tgttgctatt atgtttgggc
     3661 tctaaatagg aaatcactcg ttttataatt gtagtgttct gtgtatatat cccttcatca
     3721 aattgataca tagctaactt agtattttct tcatcatcaa aaagtataaa tgttaaatgc
     3781 tcattcaaaa tatatgcaca ttttataggg ctaattgttg taggctttcg ccctcgtttt
     3841 ccttcttcaa tccaattttc ttccattttt gttctttctt catgccccaa ttgattcaac
     3901 aactgtttaa catctttact cccctgtggt ataacattaa attcatctat cgatttagta
     3961 taaattgtct tttgattctt tatagcttta tctaactcaa gctgtcccca agtcgtatta
     4021 cctcgtttac tttcccattt atccgtaaga ttattgtagt ttaaaaatat acgctccatt
     4081 tgctgtttgt ttttacctgt gtaaaacgct aagtagtgta ataagctttg tactgcttcg
     4141 cttgaactct caaaatatga ttcatatgtg ccttgtaata gatctttaat tttatctttt
     4201 tgtttagatt tcatcataat gtttataata tcttcatcag ataattgtat attggtatta
     4261 gattcctctc tcacaacctc atttgctggc aaattctctt taaaatattc atcaactaga
     4321 gtatttaata cttcttgatt atcacaaata tcactttgac caattgtgca tcctgtaaca
     4381 gtcataaacc ttgctgaatc atataactct atgtctaaat ctgttctttt ctttttacgg
     4441 ttatctggta gtttaccttt aaagaaacaa tgtaatcctg taccacttgg agacttttca
     4501 caatatgtga gttgcatcat ttttagtgct aattcagaat tgatttgtcc ttttttatca
     4561 attgcattat caatatctag gcaaacaaaa ttattaccat cacttaatac aaaacctata
     4621 ccgctatatt gctcatcaac ttcatattca atacttacac tttcaaagtc acaccatgtt
     4681 tttttattgg ttgtactagc tctgtaacca ttaatactat atggtacttt tccaaaattt
     4741 tgttgcttct cgttccattc agctcgccat aatacccaat tgggcactaa ttttaattct
     4801 tgcggtattt ctaattcttt aactccaata attctatctt ttttcttaat tgccacagaa
     4861 tgcctccttt ctaatctgat ttctctgtat tttcaatgca actaatgtta aaatattaat
     4921 ggtatgattt tataaatttt tattttttta tgcgttatct tggctttggt cggcgaaaga
     4981 tgacgctttt tctaatgcat caaacatttg atcattgtga atacgtctct catgatatga
     5041 aaatccttct ttaatagcat cgtctttatt taaggtatga tttggctcat gtagatataa
     5101 atcttcaaaa tgcgagccat gattatcctt taaatcctca aacttttctt tcaatatctt
     5161 taaatcacac attaaatttt taatttccca attcatttat aattcctcca cttcaatatt
     5221 tcccacaatg taatctaatg cccactctaa cattccaatc acgtgtcctt tgcgatctgt
     5281 tgtatgttta tgttcacctt tttcgtctat gacactataa cggtaaacgt gttgtgtttc
     5341 tttcatgaca tcacttagcg tcattgttac ttcatcaaga attaaaaatg cttcatcttc
     5401 aaaatctaat tcagcaagaa tattgaacaa tccattatga actaacttta aagcatattc
     5461 atataatgct ttatcctgat aatgatagtc cttatcggat tgaaacttat cttcctcgta
     5521 taatactact tctaatattt taaccacttt tgataattga tatttttgtt tgatttccat
     5581 cttaactacc tacttttttc ttatttttaa tttctgtaat ttttgataaa tccatttcta
     5641 aacacgcaac ttgaacatct ttactaacat caggaaagta ctgttcaaat acttttggcg
     5701 gtatatttaa cattaaatta tgttgagtat ctttaatgtt gtaccagcca actactgttt
     5761 ttgtaataat aacttgtttt cgcacgttgt aatctctcct gttaaattaa atccataagt
     5821 taccatcatg ccgtacacac taaaagcgac atacatgtta gatattgcta gtaataatat
     5881 tgttaacaat gaaactaagc agatataagt taagtacatt ttcattgcct tgcctcctac
     5941 atccattttt tatgacgcgc cttcatgtac tcctcgaatc gcggaatact gataacaatc
     6001 attgttgatg ataacgagta atataaatca tcaacacctt tagaatcttt ttcccactct
     6061 tttaaaatgc gattcaccga actatatgaa attcccaaaa tactagctag tgcattaggt
     6121 ttcgcaaaca aaggattcac tacaacttgc tttggttctg taattgtatt ttctttagct
     6181 ggtacatcat gcaattttgt tctagacatt taattcacct ccttttaaga tgttttttcg
     6241 ttcttttccg ggaacgttat tggtaaaaaa aatgtctagg ttatttgttt cataacctaa
     6301 tatttttgcc attttaataa attcattcgc tccaatatct actattccat tttctctctt
     6361 tgcataaggc gttcttgttt tccaccccat tttgtgagcc atttcatctt gcgtaatacc
     6421 acaagctatt ctttccgctc tcaatctttt taaatttagt accacgttta cacctcctat
     6481 cgttctcgtt cgagaactaa ttacaattta ccatttacgt tctcgttcgt caacaatttt
     6541 tacttaaaaa aattcaaaaa gttttttctt ccttatatat tgtattcttt tgggaacggt
     6601 gttataatca aatcgttcac aaataagaac aaatattcaa ttcaggagat acaagaaatg
     6661 agaactaatg atgaaataat cacaataatt aaaacatcaa tgaaagaaca aaatatgtca
     6721 ctaagtgaat tagctcgtcg tgtaggtgta gcaaaatcag cagtatcaag atatttaaat
     6781 ttaactagag agttcccatt gaatcgtgct gaagattttg cgaaagtact tggaataaaa
     6841 acagaatatt tattaggatt tgctgaacgc gaagaatcta caaaacaaga tactatcgcc
     6901 gcgcacttag atggagattt tacagaggaa gaattaattg aaattagaaa gtatgcggag
     6961 ttagttagaa aagcacatcg aaatcagtaa ggggttattg catgtattta tacgaaaaga
     7021 tggttattga aaacaaagag ataccaattg atgatgggaa atctttaggt aattttgagg
     7081 gtctctatga caatggggtt attttaatta ataaaaattt gtcagaaaca cgtaaagcag
     7141 aagtgttata tgaggaactt gcccaccaca agttgacgta tggcaacatt ttagaccaat
     7201 caaaatggat taatagaaaa tttgaaaatt atgcacgtag acacggcttt atctcagctg
     7261 ttccattacg tgaaattgta gaagcttata attatggcgt acgtaacttg tatgagttgt
     7321 ccgagtacct acaactgagt gaaaaataca tattagaagc gatagaacaa tataaaaaga
     7381 tatacggtat tggtacccac tacggcgagt attcaattac atttgagccg ttgagagttt
     7441 ttaaatataa agaaatataa caaaggagaa atgaaaatga ataaaatatt tagagtactc
     7501 actgttagct tgtttttctt cacattttta ataaaaaaca atctagcata tgctgatgta
     7561 ggggtaatca accttagaaa cttttatact aattatcaac cagaaacgct tcaaggagtt
     7621 agttctggaa atttttctac ttctcatcaa ttagagtata ttgatggaaa atacacttta
     7681 tattcacagt ttcataatga atatgaagcg aagagattaa aagatcataa agtagatatc
     7741 tttggaataa gttactcagg tctttgtaat acaaaatata tgtatggcgg aattacgttg
     7801 gcgaatcaaa atttagataa acctagaaat atacctatta atctctgggt caatggtaag
     7861 caaaacacta tatctacaga cgaggtttct actcaaaaaa aagaggtaac tgctcaagag
     7921 attgatatta agttacgaaa atacctacaa aacgaataca atatatatgg ttttaataaa
     7981 acaaaaaaag gtcaggaata tggatatcag tcaaaattta attctggatt taacaaagga
     8041 aaaattactt tccatttaaa taatgaaact tcttttacat acgatttgtt ttacaccgga
     8101 actggtcaag cagagagttt tttaaaaatt tacaatgata ataaaactat agatgcagag
     8161 aattttcatt tggatgtaga gatttcatat gagaagactg aataaataga atttaaggag
     8221 aaaatgcaat gaaaaaat
//
LOCUS       DAKTIO010000122         8199 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.122, whole genome shotgun sequence.
ACCESSION   DAKTIO010000122 DAKTIO010000000
VERSION     DAKTIO010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8199
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.122"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..1249
                     /locus_tag="O0293_002006"
     CDS             <1..1249
                     /locus_tag="O0293_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HCY0280884.1"
                     /translation="EILDPFKGILKFQLPNDVIKRDGSYQAQVSVAELGNSDVVVVER
                     TITFNVEKSLFSKIPSETKLHYIVEFQELEKTIMDRAKAMDEAIKNGEDYASLIEKAK
                     EKGLSDIQIAKSSSIDELKQLANSRISDLENKAQAYSRTFDEQKRYMDEKHEAFKQSV
                     NSGGLVTSGSTSNWQKAKITKDDGKIMQITGFDFNNPEQRIGDSTQFIYVSQAINYPR
                     DVSTNGTVEYLVVTSDYKRMTYRPNGTNKVFVKRKEAGSWSEWSELAINDYNTPFETV
                     QSAQSKANMAESNAKLYADDKFNKRYSVIFDGTANGVGSTLYLNESLDQFILLIFYGT
                     FPGGDFTEFGSPFGGGKISLNPSNLPDGDGNGGGVYEFGLTKSSRTSLTISNDVYFDL
                     GSQRGSGANANRGTINKIIGVRK"
     gene            1249..1638
                     /locus_tag="O0293_002007"
     CDS             1249..1638
                     /locus_tag="O0293_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2977 domain-containing protein"
                     /protein_id="HCY0280885.1"
                     /translation="MQILVNKRNEIISYAIIGGFEEGIDIENLPENFSQVFRPKAFKY
                     SNGEIVFNEDYSEEKDDLHQQIDSEEQNTVASDDILRKMVASMQKQVVQSTKLSMQVN
                     KQNALMAKQLVTLNKKLEEVKGETENA"
     gene            1631..1795
                     /locus_tag="O0293_002008"
     CDS             1631..1795
                     /locus_tag="O0293_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCY0280886.1"
                     /translation="MLKLISPTFEDIKTWYQLKEYSKEDIAWYVDMEVIDKEEYAIIT
                     GEKYPENLES"
     gene            1841..2140
                     /locus_tag="O0293_002009"
     CDS             1841..2140
                     /locus_tag="O0293_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HCY0280887.1"
                     /translation="MFGFTKRHEQDWRLTRLEENDKTMFEKFDRIEDSLRTQEKIYDK
                     LDRNFEELRRDKEEDEKNKEKNAKNIRDIKMWILGLIGTILSTFVIALLKTIFGI"
     gene            2276..2578
                     /locus_tag="O0293_002010"
     CDS             2276..2578
                     /locus_tag="O0293_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCY0280888.1"
                     /translation="MDAKVITRYIVLILALVNQFLANKGISPIPVDEESVSSIILTVV
                     ALYTTYKDNPTSQEGKWANQKLKKYKAESKYRKATGQAPIKEVMTPTNMNDTNDLG"
     gene            2589..4043
                     /locus_tag="O0293_002011"
     CDS             2589..4043
                     /locus_tag="O0293_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCY0280889.1"
                     /translation="MLITKNQAEKWFDNSLGKQFNPDLFYGFQCYDYANMFFMLATGE
                     RLQGLYAYNIPFDNKAKIEKYGQIIKNYDSFLPQKLDIVVFPSKYGGGAGHVEIVESA
                     NLNTFTSFGQNWNGKGWTNGVAQPGWGPETVTRHVHYYDNPMYFIRLNFPNNLSVGNK
                     AKGIIKQATTKKEAVIKPKKIMLVAGHGYNDPGAVGNGTNERDFIRKYITPNIAKYLR
                     HAGHEVALYGGSSQSQDMYQDTAYGVNVGNKKDYGLYWVKSQGYDIVLEIHLDAAGES
                     ASGGHVIISSQFNADTIDKSIQDVIKNNLGQIRGVTPRNDLLNVNVSAEININYRLSE
                     LGFITNKNDMDWIKKNYDLYSKLIAGAIHGKPIGGVVASEVKAPVKNEKNPPVPAGYT
                     LDKNNVPYKKEQGNYTVANVKGNNVRDGYSTNSRITGVLPNNTTITYDGAYCINGYRW
                     ITYIANSGQRRYIATGEVDIAGNRISSFGKFSAV"
     gene            4433..5371
                     /gene="lukS-PV"
                     /locus_tag="O0293_002012"
     CDS             4433..5371
                     /gene="lukS-PV"
                     /locus_tag="O0293_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panton-Valentine bi-component leukocidin subunit
                     S"
                     /protein_id="HCY0280890.1"
                     /translation="MVKKRLLAATLSLGIITPIATSFHESKADNNIENIGDGAEVVKR
                     TEDTSSDKWGVTQNIQFDFVKDKKYNKDALILKMQGFINSKTTYYNYKNTDHIKAMRW
                     PFQYNIGLKTNDPNVDLINYLPKNKIDSVNVSQTLGYNIGGNFNSGPSTGGNGSFNYS
                     KTISYNQQNYISEVERQNSKSVQWGIKANSFITSLGKMSGHDPNLFVGYKPYSQNPRD
                     YFVPDNELPPLVHSGFNPSFIATVSHEKGSGDTSEFEITYGRNMDVTHATRRTTHYGN
                     SYLEGSRIHNAFVNRNYTVKYEVNWKTHEIKVKGHN"
     gene            5367..6350
                     /gene="lukF-PV"
                     /locus_tag="O0293_002013"
     CDS             5367..6350
                     /gene="lukF-PV"
                     /locus_tag="O0293_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panton-Valentine bi-component leukocidin subunit
                     F"
                     /protein_id="HCY0280891.1"
                     /translation="MDMKKIVKSSVVTSIALLLLSNTVDAAQHITPVSEKKVDDKITL
                     YKTTATSDSDKLKISQILTFNFIKDKSYDKDTLILKAAGNIYSGYTKPNPKDTISSQF
                     YWGSKYNISINSDSNDSVNVVDYAPKNQNEEFQVQQTVGYSYGGDINISNGLSGGGNG
                     SKSFSETINYKQESYRTSLDKRTNFKKIGWDVEAHKIMNNGWGPYGRDSYHSTYGNEM
                     FLGSRQSNLNAGQNFLEYHKMPVLSRGNFNPEFIGVLSRKQNAAKKSKITVTYQREMD
                     RYTNFWNQLHWIGNNYKDENRATHTSIYEVDWENHTVKLIDTQSKEKNPMS"
     gene            6599..6784
                     /locus_tag="O0293_002014"
     CDS             6599..6784
                     /locus_tag="O0293_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280892.1"
                     /translation="MFILNTQAHARLDQMAQQFEVVCNGLNENEGQAIQTMDQSASLI
                     RSNLIQVKEQLEKLAVY"
     gene            6875..7792
                     /locus_tag="O0293_002015"
     CDS             6875..7792
                     /locus_tag="O0293_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCY0280893.1"
                     /translation="MFKRTKLILIATLLLSGCSTTNNESNKETKSVPEEMEASKYVGQ
                     GFQPPAEKDVVEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVVGSGKGVEVFVHCDDH
                     DIVFNASIPFDKSIIESDSSLRSEDKGDDMSTLVGTVLSGFEYRTQKEKYDNLYKFFK
                     DNEEKYQYTGFTKEAINKTQNVGYKNEYFYITYSSRSLKEYRKYYEPLIHKNDKEFKE
                     GMEQARKEVNYAANTDTVTTLFSTKENFTKDNTVDDVIELSDKLYNFKNKPEKSTITI
                     QIGKPTINTKKAFYDDNDPIEYGVYRKDE"
     gene            7849..>8199
                     /locus_tag="O0293_002016"
     CDS             7849..>8199
                     /locus_tag="O0293_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HCY0280894.1"
                     /translation="MFKKAKLILIATLLLSGCSAMNNESKKDTNTETNTETKSVPEEM
                     EASKYVGQGFQPPAEKDAIEFVKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGVE
                     VYVHCEDHGIVFNAS"
BASE COUNT         3197 a         1073 c         1522 g         2407 t
ORIGIN      
        1 agaaatatta gatccattta aaggtatttt aaaatttcaa ttacctaatg atgtaattaa
       61 aagagatgga agttatcaag ctcaagtttc ggttgcagaa ttaggtaatt cagacgtggt
      121 agttgtagag agaactatca catttaacgt tgaaaaaagt ttgtttagca agattccctc
      181 tgaaacaaaa ctacactata ttgttgagtt tcaagaatta gaaaaaacta ttatggatcg
      241 cgcgaaagca atggacgagg ctataaaaaa tggtgaggat tatgcgagtc tgattgaaaa
      301 agctaaagaa aaaggtctat cagatattca aatagcaaaa tcttcaagta ttgatgaatt
      361 aaagcaactt gctaatagcc gtatatctga tttggaaaat aaagcgcaag catattcaag
      421 aacattcgat gagcaaaagc gatatatgga tgagaaacat gaagccttca agcagtcagt
      481 gaatagtggt ggtttagtca caagtggttc tacttcaaat tggcaaaaag ctaagattac
      541 taaagatgat ggtaagataa tgcagattac tggatttgat tttaataatc cagaacaaag
      601 aataggtgat tcaacccaat ttatttatgt ttcgcaagct ataaattatc caagagatgt
      661 tagtactaac ggtactgtcg aatatttagt agtaacttca gattacaagc gtatgactta
      721 tcgaccgaac ggtacaaata aagtgtttgt taaaagaaaa gaagcgggtt catggtctga
      781 gtggtcagaa ttagctatta atgattacaa tacacctttt gaaactgttc aaagtgccca
      841 atcaaaagct aatatggccg aaagtaacgc taaattatac gcagatgaca agtttaataa
      901 aaggtattcg gttatttttg atggaacagc aaatggtgtg ggctctacat tgtacttaaa
      961 tgagagttta gaccaattta ttttattaat tttttatggg acttttccag gtggtgactt
     1021 tacagagttt ggcagtcctt ttggaggagg aaagatttca ttgaatccct caaatcttcc
     1081 agatggtgat ggaaatggtg gaggtgttta tgagtttgga ttaactaaat ctagtcgtac
     1141 atctttaact atatcaaacg atgtctattt cgacttagga agtcaaagag gctctggtgc
     1201 gaacgcaaat agagggacaa ttaacaaaat tataggagtg agaaaataat gcaaatatta
     1261 gttaacaagc gtaatgagat aatttcatac gctatcattg gcggctttga agaaggtatt
     1321 gatattgaaa atttaccaga aaatttctct caagttttta gacctaaagc ctttaaatat
     1381 tcaaatgggg aaatagtttt taacgaagat tattcagaag aaaaagatga cttgcatcaa
     1441 cagattgaca gtgaagaaca aaacacagtc gcttctgatg acatcttacg aaaaatggtt
     1501 gctagtatgc agaaacaagt tgttcaaagt acaaagttat cgatgcaagt taataagcaa
     1561 aatgcactaa tggcaaaaca acttgtgaca cttaataaaa aattagaaga ggttaaagga
     1621 gagactgaaa atgcttaaat taatttcacc aacattcgaa gatattaaaa catggtatca
     1681 attgaaagaa tatagtaaag aagatatagc gtggtatgta gatatggaag ttatagataa
     1741 agaggaatat gcaattatta caggagaaaa gtatccagaa aatctagagt cataggttat
     1801 aatcttatgg ctttttaatt tgaataaagt gggtggtgta atgtttggat ttaccaaacg
     1861 acacgaacaa gattggcgtt taacgcgatt agaagaaaat gataagacta tgtttgaaaa
     1921 attcgacaga atagaagaca gtctgagaac gcaagaaaaa atttatgaca agttagatag
     1981 aaatttcgaa gaactaaggc gtgacaaaga agaagatgaa aaaaataaag agaaaaatgc
     2041 taaaaatatt agagacatca agatgtggat tctaggatta atagggacga ttctaagtac
     2101 atttgttata gccttgttaa aaactatttt tggcatttaa aggaggtgat caccatgctt
     2161 aagggaattt taggatatag cttttggtcg tgtttctggt ttggtaagtg taagtaatat
     2221 ttaagagtca gtgcttcggc actggctttt tattttggat aaaaggagca aacaaatgga
     2281 tgcaaaagta ataacaagat acatcgtatt gatcttagca ttagtaaatc aattcttagc
     2341 gaataaaggt ataagtccga taccagtaga tgaagaaagt gtttcatcga ttatcttaac
     2401 agttgttgct ttatatacta catataaaga taatccaaca tctcaagaag ggaaatgggc
     2461 gaatcaaaaa ttaaagaaat ataaagctga aagtaaatat agaaaagcaa cagggcaagc
     2521 gccaattaaa gaagtaatga cacctacgaa tatgaacgac acaaatgatt tagggtaggt
     2581 gttgaccaat gttgataaca aaaaaccaag cagaaaaatg gtttgataat tcattaggga
     2641 agcagttcaa tcctgatttg ttttatggat ttcagtgtta tgattacgcc aatatgttct
     2701 ttatgttagc gacaggcgaa aggctgcaag gtttatatgc ttataatatc ccgtttgata
     2761 ataaagcaaa gattgaaaaa tatggtcaaa taattaaaaa ctatgacagc tttttaccgc
     2821 aaaagttgga tattgtcgtt ttcccgtcaa agtatggtgg cggagctgga cacgttgaaa
     2881 ttgttgagag cgcaaattta aacactttca catcatttgg tcaaaactgg aacggtaaag
     2941 gttggactaa tggcgttgcg caacctggtt ggggtcctga aactgtgaca agacatgttc
     3001 attattatga caatccaatg tattttatta ggttaaactt ccctaacaac ttaagcgttg
     3061 gcaataaagc taaaggtatt attaagcaag cgactacaaa aaaagaggca gtaattaaac
     3121 ctaaaaaaat tatgcttgta gccggtcatg gttataacga tcctggagca gtaggaaacg
     3181 gaacaaacga acgcgatttt atacgtaaat atataacgcc taatatcgct aagtatttaa
     3241 gacatgcagg acatgaagtt gcattatacg gtggctcaag tcaatcacaa gatatgtatc
     3301 aagatactgc atacggtgtt aatgtaggca ataaaaaaga ttatggctta tattgggtta
     3361 aatcacaggg gtatgacatt gttctagaaa tacatttaga cgcagcagga gaaagcgcaa
     3421 gtggtgggca tgttattatc tcaagtcaat tcaatgcaga tactattgat aaaagtatac
     3481 aagatgttat taaaaataat ttaggacaaa taagaggtgt aacacctcgt aacgatttac
     3541 taaatgttaa tgtatcagca gaaataaata tcaattatcg cttatctgaa ttaggtttta
     3601 ttactaataa aaatgatatg gattggatta agaaaaatta cgacttgtac tctaaactaa
     3661 tagctggtgc gattcatggt aagccaattg gtggagtggt agctagtgag gtcaaagcgc
     3721 cagttaaaaa cgaaaagaat ccgccagtgc cagcaggtta tacactcgat aagaataatg
     3781 tcccttataa aaaagaacaa ggcaattaca cagtagctaa tgttaaaggt aataatgtaa
     3841 gagacggtta ttcaactaat tcaagaatta caggggtatt acccaacaac acaacaatta
     3901 cgtatgacgg tgcatattgt attaatggtt atagatggat tacttatatt gctaatagtg
     3961 gacaacgtcg ctatattgcg accggagagg tagacatagc aggcaaccga ataagcagtt
     4021 ttggtaagtt tagtgcagtt tgataattgt atatgatgaa tcttaggcag gtacttcggt
     4081 acttgcctat tatttaaaat taataaacag ttaattttta catgaatata ttaaatttta
     4141 aaaaaacaaa cgtttttagt atataaatta ttttgtgttc gtattgtgtg ctatgattaa
     4201 aaagttgtta tggtcaacta tatcgtggtt ttatgtttat tatcaatcaa aatataaatt
     4261 atttataatt tgtttggtaa tgaacgggtt tttttcgaaa taatagtaaa aaaacacatt
     4321 tgtagatatt ttaaactcgg taaatctttt aataaatatt taattttatt aaaagttaaa
     4381 aaggtttaat ataaaaatgt aataaaattt ataaagaaag gaaatgattt ttatggtcaa
     4441 aaaaagacta ttagctgcaa cattgtcgtt aggaataatc actcctattg ctacttcgtt
     4501 tcatgaatct aaagctgata acaatattga gaatattggt gatggcgctg aggtagtcaa
     4561 aagaacagaa gatacaagta gcgataagtg gggggtcaca caaaatattc agtttgattt
     4621 tgttaaagat aaaaagtata acaaagacgc tttgatttta aaaatgcaag gttttatcaa
     4681 ttcaaagact acttattaca attacaaaaa cacagatcat ataaaagcaa tgaggtggcc
     4741 tttccaatac aatattggtc tcaaaacaaa tgaccccaat gtagatttaa taaattatct
     4801 acctaaaaat aaaatagatt cagtaaatgt tagtcaaaca ttaggttata acataggtgg
     4861 taattttaat agtggtccat caacaggagg taatggttca tttaattatt caaaaacaat
     4921 tagttataat caacaaaact atatcagtga agtagaacgt caaaattcaa aaagtgttca
     4981 atggggaata aaagctaatt catttatcac atcattaggt aaaatgtctg gacatgatcc
     5041 aaatttattt gttggatata aaccatatag tcaaaatccg agagactatt ttgttccaga
     5101 caatgaatta cccccattag tacacagtgg tttcaatcct tcatttattg caactgtttc
     5161 tcatgaaaaa ggctcaggag atacaagtga atttgaaata acgtatggca gaaatatgga
     5221 tgttactcat gctactagaa gaacaacaca ctatggcaat agttatttag aaggatctag
     5281 aatacacaac gcatttgtaa acagaaatta cacagttaaa tatgaagtga actggaaaac
     5341 tcatgaaatt aaagtgaaag gacataattg atatgaaaaa aatagtcaaa tcatcagttg
     5401 ttacatcaat tgcattgctt ttgctatcca atacagttga tgcagctcaa catatcacac
     5461 ctgtaagtga gaaaaaggtt gatgataaaa ttactttgta caaaacaact gcaacatcag
     5521 attccgataa gttaaaaatt tctcagattt taacttttaa ttttattaaa gataaaagtt
     5581 atgataaaga tacattaata ctcaaagctg ctggaaacat ttattctggc tatacaaagc
     5641 caaatccaaa agacactatt agttctcaat tttattgggg ttctaagtac aacatttcaa
     5701 ttaattcaga ttctaatgac tcagtaaacg ttgtagatta tgcacctaaa aatcaaaatg
     5761 aagaatttca agtacaacaa acggtaggtt attcttatgg tggagatatt aatatctcta
     5821 acggcttatc aggtggaggt aatggttcaa aatctttttc agagacaatt aactataaac
     5881 aagaaagcta tagaactagc ttagataaaa gaactaattt caaaaaaatt ggttgggatg
     5941 ttgaagcaca taaaattatg aataatggtt ggggaccata tggcagagat agttatcatt
     6001 caacttatgg taatgaaatg tttttaggct caagacaaag caacttaaat gctggacaaa
     6061 acttcttgga atatcacaaa atgccagtgt tatccagagg taacttcaat ccagaattta
     6121 ttggtgtcct atctcgaaaa caaaacgctg caaaaaaatc gaaaattact gttacttatc
     6181 aaagagaaat ggatagatat acaaactttt ggaatcaact tcactggata ggtaataatt
     6241 ataaagatga aaatagagca actcatacat caatttatga agttgattgg gaaaatcata
     6301 cagttaaatt aatagatact caatctaagg aaaaaaatcc tatgagctaa acagatagat
     6361 aatcaaaaaa tcttaaatat gttaaaattt acaaacactt tctttctata ttagggtaac
     6421 cacgtcttaa ttgacgtggt tattttttca gggcaaaaaa agggcggatt atttaaataa
     6481 ggacaaacac ttgtggaaaa tttaaaaggt taaaaataat aaagaacttg gtataacaag
     6541 ggttttatac atttgcgtac aacgacgaaa tgtcaattta ccatcacatt atgacgatat
     6601 gtttatttta aacacacaag ctcatgcgcg tcttgatcaa atggcacaac agtttgaagt
     6661 tgtttgtaat ggcttgaacg aaaatgaagg acaagcaatt caaacgatgg atcaatctgc
     6721 ctctctaata cggtcaaact taattcaagt taaagaacaa ttagaaaaac tagctgtata
     6781 ctaattaatt tattaaatgc tacttgtttt cattgagaat aagtagcttt ttcaaacata
     6841 aaagttttac aaacacataa atgggtgatg agctatgttt aaaagaacta aactaatctt
     6901 aatagcaaca ttactgctat caggatgttc aactaccaat aacgaatcta ataaagaaac
     6961 aaaatcagtg ccagaagaaa tggaagcttc aaaatatgta ggtcaaggct tccaaccacc
     7021 tgcagaaaaa gatgtggttg aatttgcgaa aaagcataaa gataaaattg ccaaacgagg
     7081 cgaacaattt tttatggata atttcggtct aaaagttaaa gctaccaatg ttgtaggtag
     7141 tggaaaaggc gtagaagtat tcgtgcattg tgatgaccac gatatcgtat ttaatgcgag
     7201 tattccattt gataaatcaa taattgagag tgatagctca ttaagaagtg aggacaaagg
     7261 cgatgatatg agtactttag ttggtacagt gttgagtggc tttgaatatc gaacacaaaa
     7321 agaaaagtat gacaatttat ataaattttt caaagataat gaagagaaat accaatatac
     7381 aggctttaca aaagaagcaa taaacaagac gcaaaatgtc ggatataaaa atgaatattt
     7441 ttatatcact tactcgtcaa gaagcttaaa agaatatcgt aagtattacg aaccattgat
     7501 tcataaaaat gataaagaat ttaaagaagg aatggaacaa gccagaaaag aagtgaatta
     7561 cgctgctaat acagatacag taacaacatt gtttagtaca aaggaaaatt ttactaaaga
     7621 caatacagtt gatgatgtaa ttgaactgag tgataaacta tataatttta aaaataagcc
     7681 agaaaaatct acaattacaa ttcaaatagg aaagcctact attaatacta aaaaagcctt
     7741 ttatgatgat aatgatccaa tagaatatgg agtgtatcgt aaagatgaat aaattttttt
     7801 aaaatgcttt ttaatattct atataaacac ataaatgggt gatgagctat gtttaaaaag
     7861 gcgaaattaa tcttaatagc aacgctactg ttatcaggat gttcagcgat gaataatgaa
     7921 tcaaaaaaag acacaaatac agaaacaaat acagaaacaa aatcagtacc agaagaaatg
     7981 gaagcttcaa aatatgtagg acaaggcttt caaccacctg cagaaaaaga tgcgattgaa
     8041 tttgtgaaga agcatcgtaa agaatttgaa aaagtaggtg aacaattctt taaagataac
     8101 tttggactaa aagttaaagc tacaaatgtt gtaggtaaag atgatggtgt agaagtttat
     8161 gtgcattgcg aagatcatgg cattgtattt aatgcaagt
//
LOCUS       DAKTIO010000123         8098 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.123, whole genome shotgun sequence.
ACCESSION   DAKTIO010000123 DAKTIO010000000
VERSION     DAKTIO010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8098
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.123"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(2..808)
                     /gene="yjbH"
                     /locus_tag="O0293_002017"
     CDS             complement(2..808)
                     /gene="yjbH"
                     /locus_tag="O0293_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease adaptor protein YjbH"
                     /protein_id="HCY0280895.1"
                     /translation="MAGELRIMENKSREDINLSPVSKIEIYSFFDPFSSDCFKLSAIL
                     SKLRIEYNQYIRIRHILNPSLKVLTKCQAQSTSNFDNIALAYKAAELQGRVRAERFIH
                     LMQNEIIPKRDIITESMICDCIQNAGIDLEVFKDDLQKSKLTESLKIDLHIAREMEIE
                     QAPSLVFFSEDVHEEGLKVEGLYPYHIYTYIINELMGKPIEKNLPPKLETYIQQQQLV
                     TMEELLTIYEWPEKLLNKELKKLAIQQKIEKLKYPDGDFWKSKMPKIKSK"
     gene            complement(831..1196)
                     /locus_tag="O0293_002018"
     CDS             complement(831..1196)
                     /locus_tag="O0293_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="truncated hemoglobin"
                     /protein_id="HCY0280896.1"
                     /translation="MTTTPYDIIGKEALYDMIHYFYTLVEKDERLNHLFPGDFAETSR
                     KQKQFLTQFLGGPNIYTEEHGHPMLRKRHIDFTITEFERDAWLENMQTAINRAAFPQG
                     VGDYLFERLRLTANHMVNS"
     gene            complement(1300..1893)
                     /locus_tag="O0293_002019"
     CDS             complement(1300..1893)
                     /locus_tag="O0293_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HCY0280897.1"
                     /translation="MATNHEIEFKQMITASIYNKLQEKYFKDSVLFKQVNYYIDTPDF
                     KLKEHRSALRIRVKDNQYEMTLKTPAKVGLLEYNYIVDIKPEMNLIISNDNLPDDIRQ
                     IIVEQFGVKDTTLSILGALTTYRQETKYKGDLLVLDKSEYFDTTDYELEFEVKDYNQG
                     LQKFQSLLNELNLEHHQPLNKVQRFFKKKETLSNNIN"
     gene            2079..2426
                     /locus_tag="O0293_002020"
     CDS             2079..2426
                     /locus_tag="O0293_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280898.1"
                     /translation="MRLYINEIKIKDDILYCYTEDSIKGLSEVGQMLVDSDNYAFAYT
                     LDDGKAYAYLIFVQETWTMLHENMTKKIIINDELELTEFHQELTYILDNIKGNNNYGK
                     EFVATVEETFDIE"
     gene            2443..3078
                     /locus_tag="O0293_002021"
     CDS             2443..3078
                     /locus_tag="O0293_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCY0280899.1"
                     /translation="MNQWDQFLTPYKQAVDELKVKLKGMRKQYEVGEQASPIEFVTGR
                     VKPIASIIDKANKRQIPFDRLREEMYDIAGLRMMCQFVEDIDVVVNILRQRKDFKVIE
                     ERDYIRNTKESGYRSYHVIIEYPIETLQGQKFILAEIQIRTLAMNFWATIEHTLRYKY
                     DGAYPDEIQHRLERAAEAAYLLDEEMSEIKDEIQEAQKYYTQKRSKKHEND"
     gene            3095..3904
                     /locus_tag="O0293_002022"
     CDS             3095..3904
                     /locus_tag="O0293_002022"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HCY0280900.1"
                     /translation="MRYTILTKGDSKSNALKHKMMNYMKDFRMIEDSENPEIVISVGG
                     DGTLLQAFHQYSHMLSKVAFVGVHTGHLGFYADWLPHEVEKLIIEINNSEFQVIEYPL
                     LEIIMRYNDNGYETRYLALNEATMKTENGSTLVVDVNLRGKHFERFRGDGLCVSTPSG
                     STAYNKALGGALIHPSLEAMQITEIASINNRVFRTVGSPLVLPKHHTCLISPVNHDTI
                     RMTIDHVSIKHKNVNSIQYRVANEKVRFARFRPFPFWKRVHDSFISSDEER"
     gene            3901..4755
                     /locus_tag="O0293_002023"
     CDS             3901..4755
                     /locus_tag="O0293_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCY0280901.1"
                     /translation="MKFKYHISQQETVKTFLARHDFSKKTVSAIKNNGALIVNDEPVT
                     VRKQLMTNDILEIHLPREIPSVNLIPYARKLEVLYEDAFIIIVTKPNNQNCTPSREHP
                     HESLIEQVLYHCQEHGENINPHIVTRLDRNTTGIVIFAKYGHIHHLFSKVNLKKIYTC
                     LVYGKTHTSGIIEANIRRSKDRIITREVASDGKYAKTSYEVINQNDKYSLCKVHLHTG
                     RTHQIRVHFQHIGHPIVGDSLYDGFHDKIHGQVLQCTQIYFVHPINKNNIYITIDYKQ
                     LLKLFNQL"
     gene            4776..6161
                     /gene="mgtE"
                     /locus_tag="O0293_002024"
     CDS             4776..6161
                     /gene="mgtE"
                     /locus_tag="O0293_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HCY0280902.1"
                     /translation="MSMNTDEKERVQEELYDQTLLDQYLENDDIDQFRDEFLALHTYE
                     QSEYFEDTTDENRQKIFQYLSPEEVANFFDQLDIDDDEYELLFDKMNATYASHILEEM
                     SYDNAVDILNELTKPKVASLLTLMNKDDANEIKALLHYDEDTAGGIMTTEYLSLKAHT
                     PVKEALLLVKAQAPDAETIYVIFVVDDDGKLVGVLSLRDLIVAENDAYIEDIMNERVI
                     SVNVADDQEDVAQVMRDYDFMAVPVIDYQEHLLGIITIDDILDVMDEEASEDYSRLAG
                     VSDIDSTNDSIIKTALKRLPWLIILTFLGMITATILGRFEKTLENVALLAAFIPIISG
                     MSGNSGTQSLAVSVRNITTGEINEQSKFRIALREAGSGVLSGVVCSTILFTIIVAIYH
                     QPLLALIVAGSLTCAMTVGTFVGSMIPLLMNKLNIDPAVASGPFITTINDIISMLIYF
                     GLATSFMAYLI"
     gene            6171..8015
                     /locus_tag="O0293_002025"
     CDS             6171..8015
                     /locus_tag="O0293_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter family
                     protein"
                     /protein_id="HCY0280903.1"
                     /translation="MEFLSLVIVVLAAFLTPIIVNRLNINFLPVVVAEILMGIVIGNS
                     FLNIVERDSILNILSTLGFIFLMFLSGLEIDFKAFKKDKRARQGQNDDESSIPGHLNL
                     ALTVFAFIMIISILLAYVFKWLGLVDDVLLMVIIISTISLGVVVPTLKEMNIMRTTIG
                     QFILLVAVLADLVTMILLTVYGAINGQGGSTIWLIGILVVFTAISYILGVQFKRMSFL
                     QKLMDGTTQIGIRAVFALIILLVALAEGVGAENILGAFLAGVVVSLLNPDEEMVEKLD
                     SFGYGFFIPIFFIMVGVDLNIPSLIKEPKLLIIIPILIVAFIISKLIPVMFIRRWFDM
                     KTTIASAFLLTSTLSLVIAAAKISERLNAISAETSGILILSAVITCVFVPIIFKKLFP
                     VPDEFNRKIEVSLIGKNQLTIPIAQNLTSQLYDVTLYYRKDLSDRRQLSDDITMIEIA
                     DYEQDVLERLGLFDRDIVVCATNDDDINRKVAKLAKAHQVERVICRLESTTDDTELVD
                     SGIEIFSSYLSNKILLKGLIETPNMLNLLSNVETSLYEIQMLNYKYENIQLRNFPFGG
                     DIIFVRIIRNNESIVPHGDTQLRYGDRLIVTGAKEYVDELKQELEFYF"
BASE COUNT         2727 a         1136 c         1446 g         2789 t
ORIGIN      
        1 tttattttga tttgatttta ggcattttag atttccagaa atcgccatct ggatatttaa
       61 gtttttcaat tttttgttga atggctaact tctttaactc tttgtttaaa agtttttctg
      121 gccattcata aatagtaagt aattcttcca tcgttacaag ttgttgttgc tgtatataag
      181 tttctaattt aggaggaaga ttcttttcga taggtttacc catcaattca ttaattatat
      241 aagtatagat gtgatatggg tataatcctt cgacttttaa accttcttca tgaacatctt
      301 cactaaagaa aacgagagaa ggggcttgtt cgatttccat ttctcttgca atatgcaaat
      361 caattttcaa gctttcggtt agtttacttt tttgtaagtc gtctttaaat acttctaaat
      421 caatacctgc attttgaata cagtcacaaa tcattgattc tgtaataata tctcttttag
      481 gtatgatttc attttgcatt aaatgtataa atcgttcggc acgtacacga ccttgtaact
      541 cagctgcttt ataagctagg gcgatgttat caaagttgga tgtactttga gcttggcatt
      601 tcgttaatac ttttaacgaa ggatttaata tatgtctgat acgtatatat tgattatatt
      661 caattcttaa tttggataag attgctgata atttgaagca atcggagcta aatggatcga
      721 aaaatgaata aatttcgatt ttacttacag gtgatagatt gatatcttca cgactcttat
      781 tttccattat tcgtaattct ccagccatgt ttattcacct acaattaatt ttaggaattc
      841 accatatgat tagcagttaa tcttaagcgc tcaaataaat aatctccaac accttgtgga
      901 aacgcagcgc gattaattgc tgtctgcata ttttctaacc atgcatctct ttcaaattca
      961 gtgattgtaa aatctatatg tctttttctt agcataggat gtccgtgttc ttcggtataa
     1021 atgtttggac cgcccaaaaa ctgtgttaaa aattgttttt gtttacgact tgtttctgca
     1081 aaatctcctg gaaacaggtg attaagtcgt tcatcttttt ctacaagggt gtaaaaataa
     1141 tgaatcatat cgtataacgc ttctttacca atgatgtcat atggtgttgt tgtcatttaa
     1201 tcacccattt tcaaaaattt actgttacga acttaagtta atatataact aatataacat
     1261 gattttaaac atttgaaaga aatatgcata tttgccaatt taatttatat tgtttgaaag
     1321 tgtttctttt ttcttgaaaa aacgttgaac tttatttaaa ggttgatgat gttcgaggtt
     1381 tagttcgttt aataaagatt ggaacttttg taaaccttga ttatagtctt taacttcgaa
     1441 ctctaactca taatccgtag tatcgaaata ctcactttta tctaaaacca gtaaatcacc
     1501 tttatattta gtttcttggc gatatgtcgt taatgcacca agtattgata aagttgtatc
     1561 ttttacacca aactgttcaa ctataatttg acgaatgtca tctggaagat tgtcgtttga
     1621 aataatcaag ttcatctctg gtttaatgtc gacgatatag ttgtattcta atagaccaac
     1681 ctttgctggt gtctttaaag tcatttcata ttgattgtct ttaactctta tgcgtagtgc
     1741 agagcgatgt tcctttaatt tgaaatcggg tgtatcaata tagtaattga cttgcttaaa
     1801 aagcacactg tctttaaaat atttctcttg caatttatta tagattgatg cagttatcat
     1861 ttgtttaaat tctatttcat gatttgttgc catgatatgt atacacctcg tatcaaattc
     1921 aatttatctt aactatatta tgaatgacaa agttgaattt taaaagtaat ttcctttatc
     1981 tattatcaat gttaatttga ccattaaaaa tagtgttcgt aagtgttttg tattattgaa
     2041 ttgtgttaaa atgttatgga ataagaggag gattaagcat gcgtttatat attaatgaaa
     2101 ttaaaattaa agatgacata ctttattgtt atacagaaga ttctattaaa ggattatctg
     2161 aagtaggaca aatgctcgtt gatagtgata attatgcctt tgcgtataca ttagatgatg
     2221 gtaaagcgta tgcttatctc attttcgtac aagaaacatg gacgatgttg catgaaaaca
     2281 tgactaaaaa aattattatc aatgatgaac tagaattgac tgaattccac caagaactta
     2341 cttatatttt agacaacata aaagggaata ataattatgg taaggaattt gttgcaaccg
     2401 ttgaagaaac attcgacatt gaataaagcg gggtgaagca ctatgaatca atgggatcag
     2461 ttcttaacac cttataagca agcggttgat gagttgaaag tgaaacttaa aggcatgcgc
     2521 aaacaatatg aagttggtga acaagcgtcg ccaatagaat ttgttactgg tcgtgttaaa
     2581 ccaatcgcta gtattataga taaggcaaac aaacgacaaa taccatttga taggttaaga
     2641 gaagaaatgt acgatatcgc tggtttaaga atgatgtgcc aatttgttga agatattgat
     2701 gttgtcgtca atattttaag acaaagaaaa gattttaaag taattgaaga acgagattat
     2761 attcgtaaca ctaaagaaag tggttaccgc tcgtatcatg tcattattga atatccaatt
     2821 gaaacattac aaggccaaaa atttatattg gctgagattc agattcgtac attagcaatg
     2881 aatttctggg caacgattga acatacttta cgatataaat atgatggtgc ttatccggat
     2941 gaaattcaac atcgtttgga aagagcggca gaagcagcgt atttacttga tgaagagatg
     3001 tctgaaatta aagatgaaat tcaggaagct caaaaatatt acacgcaaaa acgttctaaa
     3061 aaacatgaaa atgattaacg aggtgttata aatcatgcgt tatacaattt taactaaagg
     3121 tgactccaag tctaatgcct taaagcataa aatgatgaac tatatgaaag attttcgcat
     3181 gattgaggat agtgaaaatc ctgaaattgt tatttcagtt ggtggtgacg gtacattact
     3241 acaagcattc catcagtata gccacatgtt atcaaaagtg gcatttgttg gagttcatac
     3301 aggtcattta ggattttatg cggattggtt acctcatgaa gttgaaaaat taatcatcga
     3361 aattaataat tcagagtttc aggtcattga atatccattg cttgaaatta ttatgagata
     3421 caacgacaac ggctatgaaa caaggtattt agcattaaat gaagcaacga tgaaaactga
     3481 aaatggctca acacttgttg tggatgttaa cttaagaggg aaacactttg agcgatttag
     3541 aggcgatgga ttatgtgtat caacaccttc gggttcaacg gcttataaca aagcgctagg
     3601 tggcgcactg atacatcctt cacttgaagc aatgcaaatt acagaaattg cctcgataaa
     3661 taatcgtgtg tttagaacgg taggatcacc acttgtatta ccaaagcatc atacatgttt
     3721 aatatcacca gttaatcatg ataccattag aatgacgata gatcatgtta gtatcaaaca
     3781 taaaaatgtt aattcaatac aataccgtgt agcaaatgaa aaagtgaggt ttgcacgttt
     3841 tagaccattc ccattctgga aacgtgtgca cgattctttc atatcaagtg atgaagaacg
     3901 atgaaattta agtatcatat atcacaacaa gaaactgtta aaactttttt agcacgacat
     3961 gatttttcta agaagacagt gagcgccatt aaaaataatg gcgctttaat tgttaatgat
     4021 gaaccagtca cagtgcgtaa gcaattaatg acaaatgata tattagaaat tcatttaccg
     4081 cgagaaatac cgagtgttaa tttaatacct tatgctcgta agctagaagt attgtatgaa
     4141 gatgctttta tcatcatagt tactaaacca aacaatcaaa attgtacgcc ttcgagagaa
     4201 catcctcatg aaagtttaat cgaacaagta ctatatcatt gtcaggaaca tggtgaaaat
     4261 attaacccac atattgttac gcgtctagat cgtaatacaa ctggtattgt gatattcgct
     4321 aaatatggac atatccatca tttattttct aaagtaaact tgaaaaaaat atatacttgc
     4381 cttgtatatg gtaaaaccca tacatctggt attattgaag ctaatattag acggtcaaag
     4441 gataggatta taactagaga agttgcctcg gatggtaaat acgctaaaac atcttatgaa
     4501 gtaataaatc agaatgataa atacagttta tgcaaagttc atttgcatac gggacgtaca
     4561 catcaaattc gtgtacattt tcaacatatt gggcatccaa ttgtgggaga ttctttgtat
     4621 gatggttttc atgacaaaat tcatggtcaa gtactgcaat gtacgcaaat atattttgtt
     4681 catccaatca ataagaacaa tatttatatt acaattgatt ataagcaatt acttaaatta
     4741 ttcaatcaac tctaattcac acagggggtg taagtatgtc aatgaacaca gatgaaaaag
     4801 agcgtgttca agaggaatta tacgaccaaa ctttattaga tcaatattta gaaaatgatg
     4861 atattgatca atttagagat gaatttctag cattacacac atatgaacaa agtgagtatt
     4921 ttgaagatac taccgatgaa aatagacaaa agatttttca atatttatca cctgaagaag
     4981 ttgcaaattt ctttgatcaa ttagatattg atgacgatga atatgagttg ctatttgata
     5041 agatgaatgc gacatacgca agtcacatat tagaagaaat gtcatacgac aatgcagtag
     5101 atattttaaa tgagttgact aaaccaaaag ttgctagtct tttaacattg atgaataaag
     5161 atgacgcgaa tgaaatcaaa gcattacttc actatgatga ggatacggcc ggcggtatta
     5221 tgacgacgga gtatttatca cttaaagcgc atacgcctgt taaagaagca ttattattgg
     5281 tcaaagcgca agcaccagac gcagaaacaa tatatgttat atttgtcgtt gatgatgatg
     5341 gtaaattagt aggtgtttta tcgctaagag atttaattgt agctgaaaat gatgcttata
     5401 ttgaagatat tatgaatgaa cgtgtcatta gtgtgaatgt agcagacgac caagaagatg
     5461 ttgctcaagt tatgagagac tatgatttca tggctgtacc tgttatagat taccaagaac
     5521 atttgcttgg tatcatcacg attgatgata ttttagacgt tatggatgaa gaggctagtg
     5581 aagactactc tcgtttagcc ggggtatcag atatcgattc gactaatgat tcaatcatta
     5641 aaacagcatt aaaacgttta ccatggttga ttattttaac atttttagga atgattactg
     5701 cgacaatttt agggagattc gaaaaaacat tagaaaatgt agcgctactc gcagcgttta
     5761 ttcctattat tagtggtatg tcaggaaatt caggtacaca atctttagcc gtttcagttc
     5821 gtaacattac gacaggggaa attaatgagc aaagtaaatt tagaattgca ttaagagaag
     5881 caggaagtgg tgtattatcg ggtgttgtat gttcaacaat attatttaca attattgttg
     5941 caatatatca tcagccactt ttagcattaa tcgttgcagg aagtttaact tgtgcgatga
     6001 cggtggggac gtttgtaggt tcgatgattc cattattgat gaataaatta aatatcgatc
     6061 cagcagtggc tagtggacca tttattacaa caattaatga tattattagt atgttgattt
     6121 attttggttt agctacatca tttatggctt acttaattta aggaggagtt atggagtttt
     6181 tatctttagt tattgttgtt ttagcagcgt ttttaactcc aataattgtc aatcgattaa
     6241 atattaattt cttgccagtt gttgttgcag aaattttgat ggggattgtg attggaaatt
     6301 catttctaaa tatagtagaa agggattcaa ttctaaatat tttatcaacg ttaggcttta
     6361 tctttttaat gtttttaagt ggtttagaaa ttgattttaa agcttttaaa aaagataaac
     6421 gcgcacgtca aggacaaaat gatgatgaat cctcaattcc agggcatctt aatctagcgt
     6481 taactgtatt tgcatttatt atgattattt cgattctttt agcgtatgta tttaaatggc
     6541 ttggattagt ggatgatgtg ttattaatgg tcattatcat ttcaactatt tccttaggcg
     6601 tagttgttcc aactttaaaa gaaatgaata ttatgagaac aactataggg caatttatcc
     6661 tattagtagc agtacttgcg gacttagtaa ctatgatttt attaacggtc tatggcgcaa
     6721 tcaatggtca aggcggcagt acaatatggt taataggtat attagttgtt ttcacagcaa
     6781 tttcatatat tttaggtgtt caatttaaaa gaatgtcatt tttacaaaaa ttgatggatg
     6841 gtacgacgca aatcggtatt cgtgcggtat ttgcattaat aatattatta gtagccctag
     6901 cagagggagt tggcgcagaa aatatattag gtgcattctt agcaggtgtc gttgtttcat
     6961 tattaaatcc agatgaagaa atggttgaaa agttagactc atttggttat gggttcttta
     7021 ttcctatttt ctttataatg gttggtgtag atttaaacat accttcatta attaaagaac
     7081 cgaaattact aattatcata ccgattttaa tcgttgcatt tatcatttca aaattaattc
     7141 cagtcatgtt tattcgacgt tggtttgata tgaaaacaac gattgcatca gcatttttat
     7201 taacatcaac attatcgctc gtgatagctg cagccaaaat ttcagaaaga ttaaatgcta
     7261 tttcagctga aacgtcaggt atattaattt taagcgcagt cattacatgt gtattcgttc
     7321 cgattatttt caaaaaactg tttccagttc cagatgagtt taaccgtaaa attgaagtta
     7381 gtttaattgg taaaaatcaa ttaacgattc ctatagcgca aaatttaaca tctcagttat
     7441 atgacgtgac attatattat cgcaaagact tgagtgatcg tcgtcaattg tcagatgata
     7501 tcacgatgat agaaattgct gattatgaac aagatgtttt agaacgacta ggtctgtttg
     7561 accgagacat agttgtttgt gctacgaatg acgatgatat taaccgaaaa gttgctaaat
     7621 tagccaaagc acatcaagtt gagcgtgtca tttgcagact tgaaagcaca acggacgata
     7681 cagagttagt tgattcaggt attgaaattt tcagtagcta cttaagtaat aaaatcttat
     7741 taaaaggttt aattgaaaca cctaacatgt tgaatttatt aagtaatgtt gaaacgtcac
     7801 tatatgaaat tcaaatgtta aattataaat atgaaaatat tcaattacgt aatttcccat
     7861 tcggaggaga catcatcttc gtgcgtatta tccgtaataa tgagtcgatt gttccgcatg
     7921 gagatacaca attgcgatat ggagatcgct taattgttac cggtgctaaa gaatacgttg
     7981 atgaattgaa gcaagagtta gaattttatt tttaacaata atgatattaa aatgcagacg
     8041 tattttagta cgacgtaaaa ttaatgattt taaaatgcta gtatgtatat gattttga
//
LOCUS       DAKTIO010000124         7968 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.124, whole genome shotgun sequence.
ACCESSION   DAKTIO010000124 DAKTIO010000000
VERSION     DAKTIO010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7968
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.124"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            183..1937
                     /gene="eap"
                     /locus_tag="O0293_002026"
                     /gene_synonym="map"
     CDS             183..1937
                     /gene="eap"
                     /locus_tag="O0293_002026"
                     /gene_synonym="map"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular adherence protein Eap/Map"
                     /protein_id="HCY0280904.1"
                     /translation="MKFKSLITTTLALGVLASTGANFNNNEASAAAKPLDKSSSSLHH
                     GYSKVHVPYAITVNGTSQNILSSLTFNKNQNISYKDLEDRVKSVLKSDRGISDIDLRL
                     SKQAKYTVYFKNGTKKVIDLKAGIYTADLINTSEIKAININVDTKKQVEDKKKDKANY
                     QVPYTITVNGTSQNILSNLTFNKNQNISYKDLEDKVKSVLESNRGITDVDLRLSKQAK
                     YTVNFKNGTKKVIDLKSGIYTANLINSSDIKSININVDTKKHIENKAKRNYQVPYSIN
                     LNGTSTNILSNLSFSNKPWTNYKNLTSQIKSVLKHDRGISEQDLKYAKKAYYTVYFKN
                     GGKRILQLNSKNYTANLVHAKDVKRIEITVKTGTKAKADRYVPYTIAVNGTSTPILSD
                     LKFTGDPRVGYKDISKKVKSVLKHDRGIGERELKYAKKATYTVHFKNGTKKVININSN
                     ISQLNLLYVQDIKKIDIDVKTGTKAKADSYVPYTIAVNGTSTPILSKLKISNKQLISY
                     KYLNDKVKSVLKSERGISDLDLKFAKQAKYTVYFKNGKKQVVNLKSDIFTPNLFSAKD
                     IKKIDIDVKQYTKSKKNK"
     gene            1924..2238
                     /locus_tag="O0293_002027"
                     /pseudo
     CDS             1924..2238
                     /locus_tag="O0293_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669703.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
     gene            complement(2562..3848)
                     /locus_tag="O0293_002028"
     CDS             complement(2562..3848)
                     /locus_tag="O0293_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCY0280905.1"
                     /translation="MNPLAQSLNEQLQQSNATAFTMLSDLGQNMFYPKGILSQSAEAK
                     STTYNATIGMATNKDGKMFASSLDAMFNDLTPDEIFPYAPPQGIEELRDLWQQKMLRD
                     NPELSIDNMSLPIVTNALTHGLSLVGDLFVNQGDTILLPEHNWGNYKLVFNTRNGANL
                     QTYPIFDKDGHYTTDSLVEALQSYNKDKVIMILNYPNNPTGYTPTHKEVTTIVDAIKA
                     LADKGTKVIAVVDDAYYGLFYEDVYTQSLFTALSNLNSNAILPVRLDGATKEFFAWGF
                     RVGFMTFGTSDQTTKEVLEAKVKGLIRSNISSGPLPTQSAVKHVLKNNKQFDKEIEQN
                     IQTLKERYEVTKEVVYADQYHSHWQAYDFNSGYFMAIKVHDVDPEELRKHLIEKYSIG
                     VIALNATDIRIAFSCVEKEDIPHVFDSIAKAIDDLR"
     gene            4048..4146
                     /locus_tag="O0293_002029"
     CDS             4048..4146
                     /locus_tag="O0293_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280906.1"
                     /translation="MMWWQEGMMTLITGGLLIIFRLWLELKWKNKK"
     gene            4389..4565
                     /locus_tag="O0293_002030"
     CDS             4389..4565
                     /locus_tag="O0293_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280907.1"
                     /translation="MKHLTKIFVVLAIILFIIGYYLQATNHESQGIKLLLAAIMFMIC
                     AFISRSNDRRKNSK"
     gene            4829..5209
                     /locus_tag="O0293_002031"
     CDS             4829..5209
                     /locus_tag="O0293_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCY0280908.1"
                     /translation="MKIILKNNSDFPIYEQIKQQVKQNILKGHVAPGEHLPSMRELAK
                     DLQVSLITTKRAYEDLEKDGFVTTIRGKGTFVKEQDSSILKEKQFFTIENLVKELVNE
                     AQAIEMSLEELQDILTFIYEEESS"
     gene            5206..6102
                     /gene="pmtA"
                     /locus_tag="O0293_002032"
     CDS             5206..6102
                     /gene="pmtA"
                     /locus_tag="O0293_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtA"
                     /protein_id="HCY0280909.1"
                     /translation="MNAIELSNVNYSSDQFNLKNISFKVPQGFVTGFIGRNGAGKTTI
                     IRLIMDLYQPQTGVIRVLEEDMALNPIELKNRIGFVYSENYFNERWTTKQLEKMIAPF
                     YRKWDHQVFEFYLEKFDLPINKSIKTFSTGMKMKLSLAVAFSHHAELYIFDEPTSGLD
                     PLARNELLEIIQQELIDENKTIFMSTHIISDLEKIADYIIHLSDGEVILNGSKEQLLQ
                     RYQVVSGAIEDLDDELASLLIYEEHKRTGFIGLTEHAQVFKEILGHKVNITTPSIENL
                     MVYLEKRKPKYHENIKLMEEGF"
     gene            6103..6783
                     /gene="pmtB"
                     /locus_tag="O0293_002033"
     CDS             6103..6783
                     /gene="pmtB"
                     /locus_tag="O0293_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtB"
                     /protein_id="HCY0280910.1"
                     /translation="MKALLIRNFKLRRYTLIIYVLLLTLYPFYIMLDSTKFFYLLQSF
                     ISPTILIIWILDAGHLFRLNRRLGGNDSYYFYMSLPVSKKQLLNANYITCIVLTLIGT
                     LVISLYAYEADVIEPNSIYFSTAYAFVISNFLSIPIAFSQFTELRRVKVPYGIYVFTI
                     IILVPFLFSIAIVLVNYFVLSQSSFPDLYSYILNIGFLIISIVILIVNYFKQLNKINT
                     RKFKGGSR"
     gene            6780..7652
                     /gene="pmtC"
                     /locus_tag="O0293_002034"
     CDS             6780..7652
                     /gene="pmtC"
                     /locus_tag="O0293_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtC"
                     /protein_id="HCY0280911.1"
                     /translation="MKLEHITKKYGSNVVLNDIDFDFGDSRIVGLIGKNGVGKTTVMK
                     VMNGNIIKFDGKVDIDNADNIGFLIEHPKLYDNKSGLYNLKLFAQVLGKGFDKAYTDK
                     IIDAFGMRPYIKKKVKKYSMGMKQKLAIAVSLMNKPKFLILDEPTNGMDPDGSIDVLT
                     TIKSLVNELDMRILISSHKLEDIELICDRAVFLRDGHFVQDVNMEEGVASDTTIVTVD
                     HKDFDRTEKYLAEHFQLQNVDKADGHLMINAQKNYQVILKALSELDIYPKYIETRKSS
                     LRDTYFNINQRGDK"
     gene            7652..>7968
                     /gene="pmtD"
                     /locus_tag="O0293_002035"
     CDS             7652..>7968
                     /gene="pmtD"
                     /locus_tag="O0293_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtD"
                     /protein_id="HCY0280912.1"
                     /translation="MRILNLVKYDFYSIFKSPLTYLAILVVSSLIATQSILMANSMDN
                     PKHIIVYGSVFAAAKWLLLIIGLMFVVKTITRDFSQGTIQLYMSKVKTRVGYIISKTI
                     SII"
BASE COUNT         2972 a         1018 c         1308 g         2670 t
ORIGIN      
        1 caaacaacat tttaggtatt gtaaattaat tagttaatca aaattaaaag aacttttaac
       61 agtgttaaaa aattattgtt aaagataatt tattattaat attcagttaa ttcaaaaaat
      121 agagaaagtc tggctataat taagttgcaa atacgaatta cacaaaaaag gagagataat
      181 ttatgaaatt taagtcattg attacaacaa ctttagcatt aggtgtttta gcatcaacag
      241 gtgcaaactt taataataat gaagcgtctg ccgcagctaa gccattagat aaatcatcaa
      301 gttcgttaca ccatggatat tctaaagtcc atgttccata tgcaatcact gtgaacggta
      361 caagccaaaa tattttatca agcttaacat ttaataagaa tcaaaatatt agttataaag
      421 atttagagga tagagttaaa tcagttttaa aatcagacag aggtattagt gatatagatt
      481 taagactatc gaagcaagcg aaatatactg tttactttaa aaatggaaca aagaaagtta
      541 tcgatttgaa agcaggtatt tacacagctg atttaattaa tacaagtgaa attaaagcta
      601 ttaatattaa cgtagatact aaaaagcaag ttgaagataa aaagaaagat aaagcaaatt
      661 accaagttcc atacacaatc actgtgaacg gtacaagcca aaatatttta tcaaacttaa
      721 catttaataa gaatcaaaat attagttaca aagatttaga ggataaagtt aaatcagttt
      781 tagaatcaaa tagaggtatt accgatgttg atttaagatt atcgaagcaa gcgaaatata
      841 cagttaattt taaaaatgga acgaagaaag ttatcgattt gaaatcaggt atttacacag
      901 cgaatttaat aaattcaagt gatattaaaa gtatcaatat taacgtagat acaaaaaaac
      961 atatcgaaaa taaagctaaa agaaactatc aagttccata ttcaattaat ttaaatggta
     1021 catctacaaa cattttatcg aatctttcat tttcaaataa accttggaca aattacaaaa
     1081 atttaactag tcaaataaaa tcagtactga agcatgatag aggtattagt gaacaagatt
     1141 taaaatatgc taagaaagct tattatactg tttattttaa aaatggtggt aaaagaatct
     1201 tacagttgaa ttcaaaaaat tacacagcaa acttagttca tgcgaaagat gttaagagaa
     1261 ttgaaattac tgttaaaaca ggaactaaag cgaaagcaga cagatatgta ccatacacaa
     1321 ttgcagtaaa tggcacatca acaccaattt tatcagattt aaaatttaca ggtgacccac
     1381 gtgtaggcta caaagatatc tctaaaaaag ttaaatcagt attgaagcat gatagaggta
     1441 tcggggaacg tgaattaaaa tatgcaaaaa aagcaactta cacagtacat tttaaaaatg
     1501 gaacgaaaaa agtgattaac ataaattcaa atattagcca actgaatctg ctttatgttc
     1561 aagatattaa aaagatagat attgatgtta aaacaggaac taaagcgaaa gcggatagct
     1621 atgtaccata tacaattgca gtaaatggca catcaacacc aattttatca aaacttaaaa
     1681 tttcgaataa acaattaatt agttacaaat atttaaatga caaagtgaaa tctgtattaa
     1741 aaagtgaaag aggcatcagt gatcttgact taaaatttgc gaaacaagca aaatatacag
     1801 tatatttcaa aaatggaaag aaacaagtag tgaatttaaa atcagacatc tttacaccta
     1861 atttatttag tgccaaagat attaaaaaga ttgatattga tgtaaaacaa tacactaaat
     1921 caaaaaaaaa taaataaatc taataatgtg aaattcccag taacaataaa taaatttgaa
     1981 aacatagttt caaatgaatt tgtgttctat aatgcaagca aaattacaat taatgattta
     2041 agtataaaac ttaaatcagc aatggcaaat gatcaaggga taactaaaca tgacatagga
     2101 cttgctgaac gcgcagtgta taaagtgtat tttaaaaatg gttcgtcaaa atatgtagac
     2161 ttaaaaactg agtataaaga tgaaagagta tttaaagcaa ctgacattaa aaaggtagat
     2221 attgaactta aattctaatc tttaatttta taataaggca tctcacaatc gtggggtgcc
     2281 ttttacattt gtagagatgt gatgcttgaa tgtttgaagt gatttgccaa aagttatata
     2341 aattggttct ataataaatc ggactgatag aaaaagtttt ttacttttca tctgtccgat
     2401 tttttgattt tgttaggaga gatgtataaa actaattagg tatagtgttt gacaagttgc
     2461 tgaataaaat atatcttatg atactttgaa agaaggaata attttaaaaa taaaaaacca
     2521 ataatccgag tcatactcat cagattattg gttgaaatta attatcttaa gtcatcaatc
     2581 gctttagcaa ttgaatcaaa tacatgtggg atatcttctt tttctacaca gctaaacgca
     2641 atacgaatat ctgttgcatt aagtgcaata acaccaattg aatatttttc aattaaatgt
     2701 ttacgaagtt cttcagggtc aacatcatga acttttattg ccataaagta tccagagtta
     2761 aagtcatagg cttgccaatg tgaatgatat tgatcagcat agacaacttc cttagtgact
     2821 tcatagcgtt cttttaatgt ttgaatattt tgttcgattt ctttatcgaa ttgtttatta
     2881 ttttttaata catgcttaac agcgctttgt gttggaagag gtccactaga aatgttactt
     2941 cgtataagac cttttacttt ggcttctaat acttctttag ttgtttgatc agacgttcca
     3001 aatgtcataa acccaacacg gaatccccat gcgaaaaatt cttttgttgc accatctaaa
     3061 cgaacaggta atattgcatt tgaatttaaa ttagataatg cagtaaataa tgattgagta
     3121 tacacatctt catagaataa accatagtat gcgtcatcca caacagctat aacttttgta
     3181 cctttatcag ctaatgcttt aattgcatcg acgatggtag tcacttcttt atgcgtaggt
     3241 gtgtaacctg tcggattatt aggataattt aaaatcataa tgactttatc tttattgtat
     3301 gattgtaaag cttctacaag tgaatcagta gtataatgcc cgtctttatc aaagatagga
     3361 tatgtttgaa ggtttgcacc atttctcgta ttgaaaacaa gtttgtaatt accccaatta
     3421 tgctctggta acaagatagt gtcaccttga tttacaaata aatcgccaac taaagataaa
     3481 ccatgtgtta atgcattcgt aacaattggt agtgacatgt tgtcgattga tagctctgga
     3541 ttgtcacgca acattttttg ttgccataaa tcacgtaatt cttcgatgcc ttgtggtggc
     3601 gcataaggga atatttcatc tggagttaaa tcattaaaca ttgcatctaa agatgatgca
     3661 aacatttttc cgtctttgtt tgtcgccata cctatagttg cattatatgt tgtactcttt
     3721 gcttcagcag attgagataa aatgcctttt ggataaaaca tattttgacc taagtcagat
     3781 aacattgtaa aggcagttgc atttgactgt tgaagttgtt catttaagct ttgggctaaa
     3841 ggattcatta ttgcttaacc tctctttaaa aaaattgtat gcctatatat tatcttcaat
     3901 tataccaaag ataaagtgtt gttggtgaat taacgaataa tatgtaaaat ctgctgccaa
     3961 aatacgaaca tatgttctgt ttttatgtta gaataggtgt aggtcatcct tcagtatgat
     4021 actttagtcg aacggaggtg tagtgtaatg atgtggtggc aagaagggat gatgacactt
     4081 attacaggtg gcttactaat tatttttcga ctttggttag aactgaaatg gaaaaataaa
     4141 aaatgatcct acacacataa ataaccccaa gtctgctggt accagacttg gggttgtgta
     4201 gtataattct gtggatacac ttaaataata tcattgaaca caatatatcg caatcaataa
     4261 tcattttgat tgaatggttt tgttgcgcta ttttagttga aagtgattag taccttgaac
     4321 ttttgaaatt caataactta ttaaaaaaat ttatatttgt tataatgtcg atgttgaggt
     4381 gaaataaatt gaaacatcta acaaaaatat ttgtagtact tgcaattatt ttatttatta
     4441 ttggttatta tttacaagca actaatcatg aaagccaagg tataaaatta ttattagcag
     4501 cgattatgtt tatgatttgt gcttttataa gtagaagtaa tgatcgacgt aaaaatagta
     4561 aatagaattg actgtttaaa acgattgttt atgttgacgc tacggttgtc acgtagagat
     4621 taaagacatt cgttttagca gtctttttta ttgaagtaga caatgcaaga ttaaatggta
     4681 gtgtcatttc attttatttt attcaactgc tagtaataca atactcagtg ttatttctga
     4741 agactggttg ataatgagac atgcatattt acaaaactgt gtatattgtg tatattgtat
     4801 atatacagaa gttgataggg ggacgttgat gaaaataatt ttaaaaaaca atagtgattt
     4861 tccgatttat gaacagatta agcaacaagt aaaacaaaat attttaaagg gacatgttgc
     4921 tcctggagag catttgccgt caatgagaga acttgccaaa gatcttcaag taagtttgat
     4981 tactaccaaa cgtgcttatg aagatttaga gaaagacggt tttgttacaa caattagagg
     5041 aaaagggacc tttgttaagg agcaagatag ttctatttta aaagagaaac aattttttac
     5101 cattgaaaat ttggttaaag aattggttaa tgaagcgcaa gccatcgaaa tgtcacttga
     5161 ggaactgcag gatattttaa cgttcattta tgaggaggaa tcatcatgaa tgccatagaa
     5221 ttaagtaatg ttaattattc aagtgatcag tttaatttaa agaatatctc tttcaaagtt
     5281 cctcaaggat tcgtcactgg ttttattggt agaaatggcg ctggtaaaac cacaataata
     5341 aggttaatta tggatttgta tcaaccacaa actggtgtta ttagagtatt agaagaggat
     5401 atggctctca atccgataga gttaaagaat agaattgggt ttgtttactc tgaaaattat
     5461 tttaatgaga gatggactac gaagcaactt gaaaaaatga ttgccccttt ttatcgtaaa
     5521 tgggaccatc aagtatttga attttatctt gaaaagttcg atttaccaat caataaatca
     5581 ataaaaacat tttcgactgg gatgaaaatg aaattatcac tagcagttgc attttcacat
     5641 catgctgaat tgtatatttt tgatgagcct acttcaggtc ttgatccctt agctagaaat
     5701 gagctattgg agataattca gcaagaatta attgatgaaa ataaaacaat attcatgtcg
     5761 acacacatta tctcagattt agaaaaaata gctgattata tcattcactt aagtgacggt
     5821 gaagttatct taaatggttc aaaagaacaa ctgttacaaa gatatcaagt tgtgagtggt
     5881 gctattgagg accttgatga tgaattagca tctctactta tttatgaaga acataagcgt
     5941 acgggattta taggattaac agaacatgcg caagtattta aagaaatttt aggtcataaa
     6001 gtgaatatca ctacaccttc tattgagaac ttaatggttt atcttgaaaa gagaaaacct
     6061 aaatatcatg aaaacatcaa attgatggaa gaaggttttt aaatgaaggc attgttaatt
     6121 aggaatttta aattacgacg atatacactt atcatctatg ttctattatt aaccttatat
     6181 cctttttata taatgcttga ctcaacaaag ttcttttact tattgcaatc ttttataagt
     6241 ccaacaatat tgattatttg gattttagat gctggtcact tatttagact caatcgtaga
     6301 ttaggtggta atgattcata ttatttttat atgagtttac cagtgtcaaa aaaacaattg
     6361 cttaatgcca actatattac atgcattgtt ttaacattaa ttggaacgct tgttattagt
     6421 ttatatgctt atgaagcaga tgtgattgaa ccaaattcaa tctatttttc aactgcatat
     6481 gcatttgtca tatctaactt cttgtctata ccaattgcat ttagtcaatt tacagaattg
     6541 cgtagagtca aagtgccata tggtatatac gtgtttacta ttatcatttt agttccattt
     6601 ttattttcaa ttgcaatagt attggtgaat tattttgttc taagtcaatc atcattccca
     6661 gatttatatt catatatttt aaatattggt tttctaatta taagcattgt tatacttatt
     6721 gttaattatt ttaaacagct caataaaata aatactagaa aatttaaagg aggcagtcga
     6781 tgaaattaga acatattaca aaaaaatacg gctcaaatgt cgttttaaat gatattgatt
     6841 ttgactttgg cgatagtaga attgtcggat taataggaaa aaacggtgtt ggtaaaacaa
     6901 ccgttatgaa agtaatgaat ggtaatatta ttaaatttga tggaaaagta gatattgata
     6961 atgcagataa tatcggtttt ttaattgagc atcctaaatt atatgataat aaatcaggat
     7021 tgtataactt gaaattattt gcacaagtat taggtaaggg ttttgataaa gcatacacag
     7081 acaaaattat agatgcattt ggtatgagac cttatattaa aaagaaagtt aagaaatatt
     7141 caatggggat gaagcaaaag ttagcaattg cagtatcttt aatgaataaa cctaaatttt
     7201 taatcttgga tgagcctaca aatggtatgg atccagatgg ctcaattgat gtgctgacta
     7261 caattaagtc tttagtaaat gaacttgata tgagaattct aatatcaagt cataagttag
     7321 aagatattga attaatttgt gatagagctg tatttttaag agacggacat tttgttcaag
     7381 atgtaaacat ggaggaaggt gttgcatctg acacaacgat agttactgtt gatcataaag
     7441 actttgatag aactgaaaaa tatcttgcag agcatttcca attacaaaat gtcgacaaag
     7501 cagacggaca tttaatgatc aatgcacaaa aaaattatca agttatacta aaagcattat
     7561 ctgaattaga tatttatccg aaatatattg aaacacgtaa aagttcattg cgtgatacgt
     7621 acttcaatat aaatcaaaga ggtgataaat aatgagaatt ttaaatttag ttaagtatga
     7681 tttttatagt atatttaaaa gtcctttaac atatttagcg atactagtcg tatctagttt
     7741 gattgcaact caaagtatac ttatggcaaa ttcgatggat aacccgaaac atattattgt
     7801 ctatggatct gtatttgctg cagcaaaatg gttattgtta ataattggat taatgtttgt
     7861 tgttaagaca attacgcgtg atttttcaca aggtacaatt caactatata tgagtaaagt
     7921 taaaacacgc gttggataca ttatttcgaa aacaatttca attatttt
//
LOCUS       DAKTIO010000125         7865 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.125, whole genome shotgun sequence.
ACCESSION   DAKTIO010000125 DAKTIO010000000
VERSION     DAKTIO010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7865
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.125"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..403)
                     /gene="ftsW"
                     /locus_tag="O0293_002036"
     CDS             complement(<1..403)
                     /gene="ftsW"
                     /locus_tag="O0293_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCY0280913.1"
                     /translation="MKNFRSILRYIGKTSKFIDYPLLVTYIVLSLIGLVMVYSASMVP
                     ATKGTLTGGIDVPGTYFYNRQLAYVIMSFIIVFFIAFLMNVKLLSNIKVQKGMIITIV
                     SLLLLTLVIGKDINGSKSWINLGFMNLQASEL"
     gene            complement(717..992)
                     /locus_tag="O0293_002037"
     CDS             complement(717..992)
                     /locus_tag="O0293_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HCY0280914.1"
                     /translation="MAKQATMKNAALKQLTKDADEILHLIKVQLDNLTLPSCPLYEEV
                     LDTQMFGLQKEVDFAVKLGLVDREDGKQIMLRLEKELSKLHEAFTLV"
     gene            1133..1615
                     /locus_tag="O0293_002038"
     CDS             1133..1615
                     /locus_tag="O0293_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280915.1"
                     /translation="MIQIKGAVKFPITLDSTTWIFDDRKVSIEDLEKGVFDGSKPIDF
                     EDNHEWNRAILEGQTNPPTLNSEIKYKKRAMLEGSFVINMTPFFKHSEPHDSATTIRL
                     SNEHDHLDIPIDLLPYLFFQFAKDGKRLYSDNGVDSFIYNQEDGYSYRINHVTHIEVI
                     "
     gene            1617..1784
                     /locus_tag="O0293_002039"
     CDS             1617..1784
                     /locus_tag="O0293_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaI family protein"
                     /protein_id="HCY0280916.1"
                     /translation="MRQVQCIICDAKVFIDERTTESKRLKNNPIRTFMCDDCKSRLDT
                     PKQRAQHYPLD"
     gene            complement(1924..3771)
                     /gene="typA"
                     /locus_tag="O0293_002040"
     CDS             complement(1924..3771)
                     /gene="typA"
                     /locus_tag="O0293_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HCY0280917.1"
                     /translation="MTNKREDVRNIAIIAHVDHGKTTLVDELLKQSGIFRENEHVDER
                     AMDSNDIERERGITILAKNTAVDYKGTRINILDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLKPVVVVNKIDKPSARPEGVVDEVLDLFIELEANDEQ
                     LEFPVVYASAVNGTASLDPEKQDDNLQSLYETIIDYVPAPIDNSDEPLQFQVALLDYN
                     DYVGRIGIGRVFRGKMRVGDNVSLIKLDGTVKNFRVTKIFGYFGLKRLEIEEAQAGDL
                     IAVSGMEDINVGETVTPHDHQEALPVLRIDEPTLEMTFKVNNSPFAGREGDFVTARQI
                     QERLNQQLETDVSLKVSNTDSPDTWVVAGRGELHLSILIENMRREGYELQVSKPQVII
                     KEIDGVMCEPFERVQCEVPQENAGAVIESLGARKGEMVDMTTTDNGLTRLIFNVPARG
                     MIGYTTEFMSMTRGYGIINHTFEEFRPRIKAQIGGRRNGALISMDQGSASTYAILGLE
                     DRGVNFMEPGTEVYEGMIVGEHNRENDLTVNITKTKHQTNVRSATKDQTQTMNRPRIL
                     TLEEALQFINDDELVEVTPESIRLRKKILNKNVREKEAKRIKQMMQENE"
     gene            3873..4064
                     /locus_tag="O0293_002041"
     CDS             3873..4064
                     /locus_tag="O0293_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaF family protein"
                     /protein_id="HCY0280918.1"
                     /translation="MKQKKSKNIFWVFSILAVVFLVLFSFAVGASNVPMMILTFILLV
                     ATFGIGFTTKKKYRENDWL"
     gene            complement(4218..5045)
                     /locus_tag="O0293_002042"
     CDS             complement(4218..5045)
                     /locus_tag="O0293_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HCY0280919.1"
                     /translation="MALYGFAQGLIQEAGIRIKQLMEQNLTIETKSNPNDLVTNVDKA
                     TEDFIFDTILETYPNHQVLGEEGHGHDIDTSKGTVWIVDPIDGTLNFVHQQENFAISI
                     GIYIDGKPYAGFVYDVMADVLYHAKVGEGAYRGSQPLKPLNDSNLRQSIIGINPNWLT
                     KPILGEIFKEIVNDSRSARAYGSAALEIVSVATGNLEAYMTPRLQPWDFAGGLVILYE
                     VNGQASNLLGEPLTISGPNSILVGNRGLHQEISNDYLEPHHDALIQLHEQRFKRKSK"
     gene            5202..5816
                     /locus_tag="O0293_002043"
     CDS             5202..5816
                     /locus_tag="O0293_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YktB family protein"
                     /protein_id="HCY0280920.1"
                     /translation="MTKYTFKPKDFKAFNVEGLDARMEALNEYIRPQLRELGEYFSDF
                     FTSQTGETFYPHVAKHARRSVNPPKDTWVAFATNKRGYKMLPHFQIGMFEDQLFVMFG
                     IMHEAKDKATRAKVFERKFKAIQQLPDDYRVCLDHMKPDKPFIKDLTDDDLIEAIQRA
                     INVKKGEFFIARAITPQDKRLKSDKAFIAFLEETFDQFLPFYSA"
     gene            6000..7352
                     /locus_tag="O0293_002044"
     CDS             6000..7352
                     /locus_tag="O0293_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCY0280921.1"
                     /translation="MNNKRHSTNEQLSLDEINNTIKFDHRSSNKQKFLSFLGPGLLVA
                     VGYMDPGNWITSMQGGAQYGYTLLFVILISSLSAMLLQSMTVRLGIATGMDLAQMTRH
                     YLSRPIAIIFWIIAELAIIATDIAEVIGSAIALNLLFNIPLIVGALITVLDVFLLLFI
                     MKYGFRKIEAIVGTLIFTVLFIFIFEVYISSPQLNAVLNGFIPHSEIITNNGILYIAL
                     GIIGATIMPHNLYLHSSIVQSRTYSRHNNEEKAQAIKFATIDSNIQLSIAFVVNCLLL
                     VLGASLFFNSNADDLGGFYDLYHALKTEPVLGATMGAIMSTLFAVALLASGQNSTITG
                     TLAGQIVMEGFLRLHIPNWLRRLITRSLAVIPVIVCLIIFKGNAAKIEQLLVFSQVFL
                     SIALPFCLIPLQLATSNKDLMGPFYNKTWVNIISWTLIIILSILNVYLIVQTFQELQS
                     "
     gene            complement(7434..7853)
                     /locus_tag="O0293_002045"
     CDS             complement(7434..7853)
                     /locus_tag="O0293_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCY0280922.1"
                     /translation="MNRKPELIMAWIANSISIIYLLVMGLSYFSLKSGNASQREELAK
                     QLSQNGGKVSLDMLQTTMGALAIILLISTLYGIFATICIKGRRKLSIILFVIAIIVSL
                     MALNLIAIVLWVIVMIMLISKKESKETTHKDDEYIYH"
BASE COUNT         2644 a         1375 c         1133 g         2713 t
ORIGIN      
        1 ataactcaga tgcctgtaag ttcataaatc ctaagtttat ccaactttta gaaccattaa
       61 tatctttacc tattactaac gtcagtaata atagtgagac gatagttata atcatacctt
      121 tttgcacttt aatattactc agtaatttaa cattcattaa aaatgcaata aaaaatacaa
      181 ttataaaact cattatgaca tatgctaatt gtcggttgta aaaatacgtt cctggaacat
      241 cgataccacc agtcaatgtg cctttagttg ctggaaccat acttgcacta tataccatca
      301 ctaatccaat taaactcaat acaatatatg taactaataa cggataatca ataaactttg
      361 aggttttacc aatataccgt aaaatacttc taaaattctt catatattca gtcatccaat
      421 tctacaattt atacttcaaa tgatttcgtt ttatacttta attttaaaaa tccatgttac
      481 ttattatata catgacacag tcataatcac aaatattaac aggtctcttg aggtaattta
      541 tagaatattt gtttacttaa tatcgtgaat tgctaaaacc caataaaagt gactgacatg
      601 tcatcaagaa gacaacataa cagccacttt aatcagtaat taaactataa atataaaatc
      661 ggcgcagctc aattgtcgat atctcacgcc attttaatat gcttatatat taaaccttaa
      721 acaagtgtaa aagcttcatg taattttgaa agttctttct caagacgtaa cataatttgt
      781 ttgccatctt cgcggtcaac taaacctaat ttaacagcaa aatcaacttc tttttgaagt
      841 ccaaacattt gtgtatctag tacttcttca tataatgggc atgaaggtaa tgttaaatta
      901 tctagttgaa ctttaatcag atgcaagatt tcatcagcat ctttagtcaa ttgtttcaaa
      961 gctgcatttt tcattgttgc ttgtttcgcc atgacacgct ccccctctat ataaattctc
     1021 aagtcttact aaaagtttat cttactaaat attaaaaagc aagtagattt aaaatgtgtc
     1081 gttagcaatt tgtgttaaaa taatgctata tacattgata gggagtggga aaatgataca
     1141 aattaaagga gccgtaaagt ttcctataac attagatagt acgacttgga ttttcgatga
     1201 tagaaaagtt agcatcgaag atttagaaaa aggtgtattt gatggttcaa aaccaatcga
     1261 ctttgaagat aatcatgaat ggaatcgtgc tatcctagaa ggacaaacta atccgcctac
     1321 tttaaattca gaaataaaat ataaaaaacg tgccatgtta gaaggttcat ttgtcattaa
     1381 tatgacacca tttttcaaac attccgaacc acatgactct gcaactacta ttcgattatc
     1441 aaatgagcat gatcatttag atataccgat agatctatta ccctatttat ttttccaatt
     1501 tgcaaaagac ggtaaacgac tttacagcga taatggtgta gatagtttta tttacaatca
     1561 agaagatggt tattcatatc gaatcaacca tgtaactcat attgaggtga tttaatttga
     1621 gacaagtgca atgcattatc tgtgatgcaa aagtatttat agatgagcgc acaactgagt
     1681 ctaaacgact taaaaacaat cctattcgaa catttatgtg tgatgattgt aaaagtcgat
     1741 tagacacacc taaacaacgt gcgcaacact atccgctcga ttaatatctg aataaacaag
     1801 ttagaaattt gaaaatgtga aagtaacagg aaataatagc atttaaacac tttttcaaat
     1861 attatatgcc aaaagagaag ctaagcaact ttcatcgctc agcttctctt ttattatgtt
     1921 aaattattcg ttttcttgca tcatttgttt gatacgcttt gcttcttttt cacgaacatt
     1981 tttgtttaaa attttctttc ttaaacgtat actttctggt gtaacctcaa caagttcatc
     2041 atcattaatg aattgtaacg cttcttccaa tgttagaata cgcggtctat tcattgtttg
     2101 tgtttggtct ttcgttgcag aacgtacgtt agtttgatgt tttgttttag tgatgttaac
     2161 agttaaatca ttttcacgat tatgttcacc aacaatcata ccttcataaa cttcagtacc
     2221 aggttccatg aagtttacac ctctatcttc aagtcccaaa atggcataag tacttgcaga
     2281 accttgatcc attgaaatta atgcaccatt acgacgaccg ccaatttgtg ctttaatacg
     2341 tggtctaaat tcttcaaatg tatggttaat aataccgtaa cctcttgtca ttgacataaa
     2401 ttcagtcgta taaccaatca taccacgagc cggtacatta aagattaaac gtgtaagtcc
     2461 attatcagtt gtagtcatat caaccatttc acctttacgt gcacctaatg attcaataac
     2521 agcacctgca ttttcttgtg gcacttcaca ttgcacacgt tcaaatggtt cacacattac
     2581 accatctatt tctttaataa ttacttgtgg ttttgaaact tgtaattcat aaccttcacg
     2641 acgcatattt tcaataagga ttgataaatg caattcaccg cgaccagcaa ctacccatgt
     2701 atctggagaa tctgtgttag aaactttcaa agatacatct gtttctaatt gttgatttaa
     2761 acgttcttga atttgacgtg ctgttacaaa gtcaccttca cggccagcaa atggagaatt
     2821 gttaacttta aatgtcattt caagagtagg ctcatcaata cgtagaactg gcaatgcttc
     2881 ttgatggtca tgtggtgtta cagtttcacc aacattaatg tcttccatac ctgaaacagc
     2941 aattaaatct ccagcttgtg cttcttcaat ttctaaacgt tttaatccaa agtaaccaaa
     3001 gattttagtt acacggaagt ttttcactgt accgtctaat ttaattagtg atacattatc
     3061 tccgacacgc attttacctc tgaatacacg accaatacca atacgtccaa cataatcatt
     3121 gtagtccaac aatgctactt ggaattgtaa tggctcatca ctgttatcaa ttggagctgg
     3181 tacataatca ataattgttt catataatga ttgtaaatta tcatcttgct tttcaggatc
     3241 taagctagct gtaccattta ctgctgaagc ataaacaaca gggaattcta attgttcatc
     3301 gtttgcttct aattcaataa ataaatctaa aacttcatct acaacaccct ctggacgtgc
     3361 tgatggttta tcaattttat taacaacaac aacaggtttc aggttttgtt ctagcgcttt
     3421 tttaagtaca aaacgtgttt gaggcattgt accttcatac gcatctacta ctaagacaac
     3481 cccatcaacc attttcataa tacgttctac ttctccacca aagtctgcat gtcctggtgt
     3541 atccaaaata ttaatacgtg tacctttata atcaacagcc gtatttttgg ctagaatcgt
     3601 aattccacgc tctctttcga tatcgttaga gtccattgca cgttcatcga catgttcatt
     3661 ttctctgaat ataccagatt gttttaacaa ctcatctact aaagttgttt taccatggtc
     3721 aacgtgagca ataattgcta tattgcggac atcttctctt ttattagtca tataaatagt
     3781 ttccttcttt cttaagtaaa atcccacttt taattacaac ttttttatta tatcatataa
     3841 ttgagaaaag ataagcaaaa ggtggggata ggatgaaaca aaaaaaatct aaaaatatct
     3901 tttgggtatt ttctatatta gctgttgttt tcttagtatt atttagtttt gctgttggtg
     3961 catcaaatgt accaatgatg attttaacat ttatattact cgttgcaacc tttggaattg
     4021 gatttactac aaagaaaaaa tatcgagaaa acgattggct ataagttata tcgacaataa
     4081 cacgcgttga tttaagacca cacatgttca tgatgaacat ttaactggtt ttatattggc
     4141 gcgtgttttt attatttaag gtataaaagc gtaatatgtt acctaattgt taggactaca
     4201 tattacgctt ttggtaatta ttttgatttt cttttaaatc gttgttcatg taattgtatt
     4261 aacgcatcat ggtggggctc taaataatca ttgctaattt cttgatggag accacgattt
     4321 ccaactaaga ttgaatttgg accactaatt gttaatggtt ctcctagtaa attggaagct
     4381 tgtccattta cttcatataa aataaccaat ccgccagcaa aatcccatgg ttgaagtctt
     4441 ggcgtcatat atgcttctaa attacctgta gcaactgaaa cgatttcaag cgctgcacta
     4501 ccatatgccc ttgcacttct agaatcatta acaatttctt taaagatttc tcctaaaatt
     4561 ggtttagtta accagttcgg attgatccca ataatgcttt gtcttagatt agaatcattc
     4621 aatggtttca agggttggct accacgatat gcaccttccc ctactttagc atgatataag
     4681 acatcagcca taacatcata tacaaaacct gcataaggtt taccatcgat ataaatacca
     4741 attgaaattg cgaaattttc ttgttgatga acaaaattca atgtaccgtc tattgggtca
     4801 acaatccata ccgtaccttt ggaagtatcg atgtcatgac catgcccttc ttcacctaat
     4861 acttgatgat tgggatatgt ttctaaaatt gtatcaaaaa tgaaatcttc tgttgcttta
     4921 tctacatttg taacaaggtc attcggattt gactttgttt caattgttaa attttgctcc
     4981 atcaattgtt taattctaat tcctgcttct tgaataagtc cttgggcaaa tccataaagt
     5041 gccataacat catctcctaa catctttatt atacatcaac attttataaa aataacttct
     5101 tatgataaaa atgaaaatac taattttaat gagagcgtct taagccgcaa ttgataaaac
     5161 atatgctaca atatttttaa acactataca ggaggtactc tatgacaaaa tatacattta
     5221 aacctaaaga tttcaaagcg ttcaacgtag aaggcttaga cgcacgaatg gaagctttaa
     5281 acgaatacat acgaccacaa ctccgtgaat taggagaata ttttagtgac ttctttacaa
     5341 gtcaaactgg tgaaacattt tatcctcacg tagcaaagca tgctagaaga agtgtgaatc
     5401 ctcctaaaga tacatgggtt gcttttgcaa caaacaaaag aggctataaa atgttacctc
     5461 atttccaaat tggtatgttt gaagatcaac tgtttgttat gtttggaatc atgcatgaag
     5521 caaaagataa agcaacacgt gcaaaagttt ttgaaagaaa atttaaagct attcaacaat
     5581 tacctgatga ttatcgtgtt tgcttagatc atatgaaacc tgataaacca tttattaaag
     5641 atttaacgga tgatgattta atagaagcga tacaaagagc catcaatgtg aaaaaaggtg
     5701 aattctttat agcgcgtgca atcacaccac aagataaaag attaaaaagt gacaaagcat
     5761 ttattgcatt tttagaagaa accttcgatc agttcttacc attttattct gcataaataa
     5821 ctttgtttaa ataatagagc acttaatcac atccatgatt tcgtgctctt ttttcttaat
     5881 attaaatcga acgttcaaca taataattca tacttttaaa aaaattaaaa taaatttagg
     5941 ttgacctaaa cattttatta ggttattata ttgtccataa gaagtagagg tgagtcaaaa
     6001 tgaataataa acgacattca acaaatgaac aattaagttt agacgaaata aacaatacaa
     6061 ttaaattcga tcatcgcagt tcaaataaac agaaattttt atcatttctt ggacctgggt
     6121 tattagtcgc tgttggttac atggatcccg gaaactggat aacatcaatg caaggtggcg
     6181 cccaatatgg ctatactttg ctattcgtaa ttcttatttc aagtttatca gcaatgttac
     6241 ttcaaagcat gacagtgaga ttaggaatag caacaggtat ggacttagct caaatgacac
     6301 gacattattt atcaagacct attgctataa tcttttggat cattgcagaa ctagcaatta
     6361 tcgctacaga tattgctgag gttattggta gtgctattgc tcttaatctc ctatttaaca
     6421 tacctttaat cgtcggtgca ctaataactg tacttgatgt atttttacta ctttttataa
     6481 tgaaatatgg ttttagaaaa attgaagcta ttgttggtac attaattttc acagtgttat
     6541 tcatctttat atttgaagtc tatatttcat caccacagtt gaatgctgtg ttaaatggat
     6601 ttataccaca tagtgaaatc attacaaata acggcattct ctatattgca ttaggtatta
     6661 ttggcgctac aattatgcct cataatttgt acttacattc atcaattgta caatctagaa
     6721 catactcaag acataacaat gaagaaaaag cgcaagcgat taaatttgct acgatagatt
     6781 cgaacattca gttatcaatc gcatttgtag tcaattgctt attattagtg ttaggagcat
     6841 cactattttt caactcaaat gctgacgatt taggtggttt ctatgattta tatcacgcct
     6901 taaaaactga acctgtacta ggtgcaacaa tgggtgcaat catgagtaca ttatttgcag
     6961 ttgcattatt agcgtcaggt caaaattcaa cgattactgg tactttagca ggacaaattg
     7021 taatggaagg atttttaaga ttacacatac caaattggtt aagacgttta attacacgtt
     7081 ctcttgctgt cattcctgtt atcgtatgct taatcatttt taaaggtaat gcagctaaaa
     7141 ttgagcaact acttgtattc tctcaagtat ttttaagtat tgctctgcca ttctgtttaa
     7201 taccattaca attggctact agtaataaag acttgatggg acctttctat aataaaacat
     7261 gggttaatat catttcatgg acacttatta ttattctaag tattttaaat gtctatttaa
     7321 tcgttcaaac attccaagaa ctacaaagtt aaaaataaaa ccattaaaat gttggttgta
     7381 tctaataatt aacaacttaa cattttaatg gtttttcttt tgctatgaac atattaatga
     7441 taaatatact catcgtcctt atgtgttgtt tcttttgatt cttttttaga aatcaacata
     7501 atcatcacga taacccataa gacaattgca attaaattaa gagccatcaa acttacaatt
     7561 atcgcgataa caaaaagtat aatcgataat tttctacgtc ctttaataca aattgtcgca
     7621 aatataccat aaagtgttga aattaataaa ataattgcta atgcacccat tgttgtctga
     7681 agcatatcta aagaaacctt gccaccgttc tgagataatt gcttcgctaa ttcttcacgt
     7741 tgtgatgcat taccactttt taaagaaaaa taggatagcc ccataactaa taaataaata
     7801 atactaatac tatttgcaat ccaagccatg attaattcag gttttctatt catcttaaaa
     7861 tcctt
//
LOCUS       DAKTIO010000126         7747 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.126, whole genome shotgun sequence.
ACCESSION   DAKTIO010000126 DAKTIO010000000
VERSION     DAKTIO010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7747
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.126"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..689)
                     /locus_tag="O0293_002046"
     CDS             complement(<1..689)
                     /locus_tag="O0293_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCY0280923.1"
                     /translation="MDLNQIKAVVFDLEGTLLDRVKSREKFIEEQYERFHDYLIHVQL
                     ADFKKAFIELDDDEDNDKPDLYKEIIKRFHVDRLTWKDLFNDFEMHFYRYVFPYYDTL
                     YTLEKLSQKGFQIGVIANGKSKIKQFRLHSLGLMHVINYLSTSETVGFRKPHPKIFED
                     MIDQLGVLPEQIMYVGDDALNDVAPARAMGMVSVWYKQEDAEIEPLEEEVDFTITTVE
                     ELLTILPIKND"
     gene            complement(744..1694)
                     /locus_tag="O0293_002047"
     CDS             complement(744..1694)
                     /locus_tag="O0293_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCY0280924.1"
                     /translation="MTFNKVLLSWIVILIITTSIYLFWQLGDINDVFNQSILINVRLP
                     RLLEALLTGMILTVAGLIFQTVLNNALADSFTLGLASGATFGSGLALFLGLTTLWIPV
                     FSITFSLITLITVLVITSVLSQGYPVRILILSGLMIGALFNSLLYFLILLKPRKLNTI
                     ANYLFGGFGDAEYSNVSIIAITFIIALFGIFIILNQLKLLQLGELKSQSLGLNVQLIT
                     YIALCIASMITAINVAYVGIIGFIGMVIPQLIRKWQWKQSLGRQLALNIVTGGQIMVM
                     ADFIGSHILSPVQIPASIIIALIGIPVLFYMLISQSKRLH"
     gene            complement(1842..2729)
                     /locus_tag="O0293_002048"
     CDS             complement(1842..2729)
                     /locus_tag="O0293_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0280925.1"
                     /translation="MKKSLIAFILIFMLVLSGCGMKDNDKQGSNDNGSSKSPYHRIVS
                     LMPSNTEILYELGLGKYIVGVSTVDDYPKDVKKGKKQFDALNLNKEELLKAKPDLILA
                     HESQKATANKVLSSLEKQGIKVVYVKDAQSIDETYNTFKQIGKLTHHDKQAEQLVEET
                     KDNIDKVIDSIPAHHKKSKVFIEVSSKPEIYTAGKHTFFNDMLEKLEAQNVYSDINGW
                     NPVTKESIIKKNPDILISTEAKTRSDYMDIIKKRGGFNKINAVKNTRIEVVNGDEVSR
                     PGPRIDEGLKELRDAIYRK"
     gene            complement(3108..3743)
                     /locus_tag="O0293_002049"
     CDS             complement(3108..3743)
                     /locus_tag="O0293_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HCY0280926.1"
                     /translation="MLGTDELYKVLYEHLGPQFWWPADNDIEMMLGAILVQNTRWRNA
                     EIALNQIKEHTHFNPNHILELPIETLQSLIHSSGFYKSKSLTIKTLLTWLARHHFNYQ
                     EINERYKGGLRKELLSLKGIGSETADVLLVYIFGRIEFIPDSYTRKIYDKLGYENTKN
                     YDQLKKVVTLPNHFTNQDANEFHALLDVFGKHYFRDKDIKNYDFLEPYFKK"
     gene            complement(4131..5792)
                     /locus_tag="O0293_002050"
     CDS             complement(4131..5792)
                     /locus_tag="O0293_002050"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCY0280927.1"
                     /translation="MNIIDQVKQTLVEEIAASINKAGLADEIPDIKIEVPKDTKNGDY
                     ATNIAMVLTKIAKRNPREIAQAIVDNLDTEKAHVKQIDIAGPGFINFYLDNQYLTAII
                     PEAIEKGDQFGHVNESKGQNVLLEYVSANPTGDLHIGHARNAAVGDALANILTAAGYN
                     VTREYYINDAGNQITNLARSIETRFFEALGDNSYSMPEDGYNGKDIIEIGKDLAEKHP
                     EIKDYSEEARLKEFRKLGVEYEMAKLKNDLAEFNTHFDNWFSETSLYEKGEILEVLAK
                     MKELGYTYEADGATWLRTTDFKDDKDRVLIKNDGTYTYFLPDIAYHFDKVKRGNDILI
                     DLFGADHHGYINRLKASLETFGVDSNRLEIQIMQMVRLMENGKEVKMSKRTGNAITLR
                     EIMDEVGVDAARYFLTMRSPDSHFDFDMELAKEQSQDNPVYYAQYAHARICSILKQAK
                     EQGIEVTAANDFTTITNEKAIELLKKVADFEPTIESAAEHRSAHRITNYIQDLASHFH
                     KFYNAEKVLTDDIEKTKAHVAMIEAVRITLKNALAMVGVSAPESM"
     gene            complement(5789..6205)
                     /locus_tag="O0293_002051"
     CDS             complement(5789..6205)
                     /locus_tag="O0293_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HCY0280928.1"
                     /translation="MDKKVSIQTKQVLKQHNEKEKFEFTTEGTWQQRQSNFIRYVEQI
                     EDATVNVTIKVDDDSVKLIRKGDINMNLHFVEGQTTTTFYDISAGRIPLEVKTLRILH
                     FVSGDGGKLKIHYELYQDNEKMGSYQYEINYKEIGE"
     gene            complement(6298..6405)
                     /locus_tag="O0293_002052"
     CDS             complement(6298..6405)
                     /locus_tag="O0293_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280929.1"
                     /translation="MKDSENLVFFGFEVLFVFYKMAYIYEALIKYGIVN"
     gene            complement(6480..7490)
                     /gene="adhP"
                     /locus_tag="O0293_002053"
     CDS             complement(6480..7490)
                     /gene="adhP"
                     /locus_tag="O0293_002053"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCY0280930.1"
                     /translation="MRAAVVTKDHKVSIEDKKLRALKPGEALVQTEYCGVCHTDLHVK
                     NADFGDVTGVTLGHEGIGKVIEVAEDVESLKIGDRVSIAWMFESCGRCEYCTTGRETL
                     CRSVKNAGYTVDGAMAEQVIVTADYAVKVPEKLDPAAASSITCAGVTTYKAVKVSNVK
                     PGQWLGVFGIGGLGNLALQYAKNVMGAKIVAFDINDDKLAFAKELGADAIINSKDVDP
                     VAEVMKLTDNKGLDATVVTSVAKTPFNQAVDVVKAGARVVAVGLPVDKMNLDIPRLVL
                     DGIEVVGSLVGTRQDLREAFEFAAENKVTPKVQLRKLEEINDIFEEMENGTITGRMVI
                     KF"
BASE COUNT         2422 a         1512 c         1006 g         2807 t
ORIGIN      
        1 ttatcatttt ttattggtaa aatggttaat aattcttcca ctgttgtaat tgtaaaatca
       61 acttcttctt cgagtggttc aatttcagca tcttcttgtt tataccatac actaaccatg
      121 cccatagctc gtgctggagc tacatcattt aacgcatcat cgccaacata cataatttgc
      181 tcaggtaata cccctagttg atcaatcata tcttcaaaaa ttttaggatg tggtttacga
      241 aaaccaactg tttctgatgt tgataaataa ttaataacat gcatcaaacc aagtgaatgt
      301 aatcgaaatt gtttaatctt agatttacca tttgcgataa caccaatttg aaagcctttt
      361 tgcgatagct tttctagtgt atacaaagta tcgtaataag gaaatacata acgataaaaa
      421 tgcatttcaa aatcattaaa taagtctttc caagttaacc tatctacatg gaaacgttta
      481 atgatttctt tatataaatc aggtttatca ttatcttcat cgtcatctag ctcaataaat
      541 gcttttttaa aatctgccag ttgaacatga attaagtagt catgaaatcg ttcatattgc
      601 tcttcgataa atttctctcg agatttaact ctgtccaaca acgtaccttc taaatcaaat
      661 acaactgctt taatttgatt caaatccatc gcttatgccc tctttatgtt tatagttatt
      721 tttattttag caaatgtcgt gtgctagtgt aaccgtttcg actgagatat tagcatgtaa
      781 aataacactg gtataccaat taatgcaatg ataatacttg ccggtatttg tactggtgac
      841 aatatatggc taccaataaa atctgccata accattattt gtcctccagt tacaatattt
      901 aaagccaatt gtcttcctaa tgattgtttc cactgccatt ttctaatgag ttgcggtatc
      961 accataccaa tgaatccaat gatgccaaca tatgcgacat ttatcgccgt tatcatagaa
     1021 gctatacata acgcgatata tgtaatcaat tgaacattta agcctagtga ctgacttttt
     1081 agttctccta attgcaataa ctttagttga ttaagaatga taaatatacc aaacaatgca
     1141 atgataaatg tgattgctat tatagataca tttgagtatt ctgcatcacc aaaaccacca
     1201 aacagataat tggcaattgt atttaatttg cgaggtttta ataaaatcaa aaaatataga
     1261 agtgaattga ataacgcacc aatcattaaa ccacttaata ttaagattct aactggatag
     1321 ccttggctca ataccgacgt aatgactaat acagttatta atgttatcaa actaaatgtt
     1381 attgaaaata caggaatcca taacgttgtt aaacctaaaa ataatgctaa tcctgaacca
     1441 aatgtagcgc cgcttgccaa tcctaatgta aagctatctg ccaatgcatt atttaaaact
     1501 gtttgaaata taaggcctgc aacagttaat atcatacctg tcaacaatgc ttctaataat
     1561 ctcggtaatc taacattgat taaaatagac tggttaaata catcattgat atcgcccaac
     1621 tgccaaaata gatatatgct agttgttata atcaatatga ctatccagct caataatact
     1681 ttattaaatg tcatagcatt ctcttttctt tcatcgataa ttacattcat tatagcaatc
     1741 acatactcgc gttgctatca ttcactaaat atattttaaa aaccccttat ctcaaacatg
     1801 tattttttac atgtagcaat aaggggcata attagaatgg tttattttct ataaattgca
     1861 tctcttaatt cttttaatcc ttcatcaata cgtggacctg gtcttgatac ttcatcacca
     1921 tttacaactt caatacgtgt attcttgaca gcattaattt tattgaatcc acctcttttt
     1981 ttgatgatat ccatataatc tgatcttgtc ttagcttccg tcgaaattaa tatatctggg
     2041 ttctttttaa taatactttc cttcgttaca gggttccaac cattaatgtc actatacaca
     2101 ttttgggctt ctaatttttc taacatatca ttaaaaaatg tatgcttccc tgctgtatat
     2161 atttcaggct ttgatgaaac ctcaataaat acttttgatt ttttatgatg agcaggaatt
     2221 gaatctatga ctttatcgat attatcttta gtttcctcaa caagttgttc agcctgctta
     2281 tcatgatgcg ttaatttccc aatttgctta aatgtgttgt aagtttcatc aattgattgt
     2341 gcatctttaa catacactac tttgatgcct tgtttctcta atgatgacaa tactttatta
     2401 gcagttgcct tttgcgactc atgcgcaaga attagatctg gctttgcctt taaaagttcc
     2461 tctttattta gattcaaagc atcaaattgt ttcttaccct ttttcacatc ttttggataa
     2521 tcatcaaccg ttgaaacacc aactatgtat ttacctaatc ctaattcata taaaatttca
     2581 gtattactag gcattaacga aacaattcta tggtacggcg atttagacga gccattatca
     2641 ttgctacctt gtttatcatt atctttcata ccacagccac tcaggacaag cataaaaatc
     2701 aaaataaaag caattaacga tttcttcaca ctatcacatc tcctttttaa tcccactttt
     2761 cgctttagcg attagaggga tttatgcagt tgtgcggtag catccaactg cgttcctctt
     2821 catctttagc gattagaggc ttttatgcag ttgtgcctta gcatccaact gcgttcctct
     2881 tcacctttag tgattagagg cttttatgca gttgctgcac aaacaactgt attcctttac
     2941 tccccttgag ctttagcgat taggggctct tatgcagttg ctgcacaaac aaacaactgt
     3001 ttttttcttt atatgcttct atttattata taagtccaca tactaaaaga aaatagtaaa
     3061 ttatttttta tatttcatat aaactcatct atctaacttc acagcgttta ctttttaaag
     3121 taaggttcta aaaaatcata attctttata tctttgtctc taaagtaatg tttaccaaat
     3181 acatctaaca gagcatgaaa ttcattagca tcttgatttg taaaatgatt tggtaatgtg
     3241 actacttttt ttaattgatc ataattttta gtgttttcat atcctaattt atcatatatt
     3301 tttcttgtat agctatctgg aataaattca atacgtccga atatataaac aagtaagaca
     3361 tctgctgttt cacttccaat acctttcaaa gataataatt cttttcttaa tccaccttta
     3421 tatcgctcat taatctcttg ataattgaaa tgatgtcgtg ctaaccatgt taataatgtt
     3481 ttaatcgtca gtgatttact tttataaaag ccacttgaat gtatcaatga ttgtaacgtt
     3541 tcaataggta gttctaatat atgatttgga ttaaaatgcg tatgttcttt aatctgattc
     3601 aatgcaattt ctgcatttcg ccatctagta ttttgaacta aaattgcacc taacatcatt
     3661 tcaatgtcat tatcagcagg ccaccaaaat tgtggtccga gatgttcata taaaacttta
     3721 tataattcat cagttcctaa catttacacc ctccatttta gagggagtgg gacagaaatg
     3781 atattatcgt aaaatttatt tcgtcgtccc accccaactt gcattgtctg tagaaattgg
     3841 gaatccaatt tctctttgtt ggggccccgc cggcaaggtt gactagaatt gaaaaaagct
     3901 tgttacaagc gaattttcgt tcagtcaact actgccatta taactttgta gagcatagaa
     3961 cattgattta tgtctcaggc tctcttatgc agttgtgcag agcatccaac tgtgttcctc
     4021 ttaaccttat tcacatacta agcattcccc aatatgtcac atacctaaaa aaacactcca
     4081 acacaaagta tctttcgaga aactctacgt tggagtgtat ataaatgttc ttacattgat
     4141 tcaggtgcgc ttacaccgac cattgccaat gcatttttca atgtaattct gaccgcttca
     4201 atcatagcaa catgtgcttt tgttttttca atatcatctg ttaacacttt ttcagcatta
     4261 tagaatttat ggaaatgaga agctaaatct tgaatataat tagtaattct atgtgccgat
     4321 ctatgctcag cagcactttc aattgtaggt tcgaaatcag ctactttttt caacaattca
     4381 atcgcttttt cattagtaat cgttgtaaaa tcattcgcag cagtcacttc aataccttgc
     4441 tctttcgctt gttttaaaat tgaacaaata cgcgcatgtg catattgagc atagtaaact
     4501 ggattgtctt gagattgctc tttcgctaat tccatatcaa aatcaaagtg actatcagga
     4561 ctacgcatag ttaagaaata acgtgcagcg tcaacgccaa cttcgtccat aatttctctt
     4621 aatgtaatcg cattaccagt acgtttactc atcttcactt ctttaccatt ttccattaaa
     4681 cgaaccattt gcatgatttg aatttctaaa cgattactat ctacaccaaa cgtttcaaga
     4741 gatgctttca aacgattaat ataaccatga tgatcagcac caaataaatc gattaaaatg
     4801 tcattaccac gttttacttt atcgaagtgg tacgcaatat ctggtaagaa atacgtatat
     4861 gtaccgtcat ttttaattaa tactctgtct ttgtcgtctt taaaatcagt tgtacgtaac
     4921 catgtagcgc catcagcttc atacgtataa cctaattctt tcatttttgc taaaacttca
     4981 agaatttctc ctttttcata taaagatgtt tcactaaacc aattatcaaa atgcgtattg
     5041 aactctgcta aatcattttt caatttagcc atttcgtatt ctacgcctaa ttttctaaat
     5101 tctttcaaac gtgcttcttc agaataatct ttaatttcag ggtgtttctc tgctaaatct
     5161 ttacctattt caataatatc ttttccatta tagccatctt ctggcattga ataactattg
     5221 tcacctaaag cttcaaagaa acgtgtttca atcgaacgcg ctaagttagt aatttgatta
     5281 ccagcatcat taatataata ttcacgtgtt acattatagc cagctgcagt taaaatatta
     5341 gctaaagcat caccaactgc tgcatttcta gcatgaccaa tatgtaaatc tcctgtaggg
     5401 ttagctgaaa catactcaag caatacattt tgaccttttg attcatttac atgtccaaat
     5461 tgatcacctt tttcaattgc ttcaggaata attgctgtta aatactgatt atctaagtaa
     5521 aaattaatga atcctggacc agcaatgtca atttgtttta catgtgcttt ttcagtatct
     5581 aagttatcaa caatcgcttg agcaatttca cgaggattac gctttgcaat cttagtcagt
     5641 accatcgcaa tattagtagc ataatctcca ttttttgtat ctttaggaac ttcaatttta
     5701 atatcaggaa tctcatctgc taatcctgct ttgttaatac ttgctgcaat ttcttctact
     5761 aatgtttgtt tcacttgatc aataatattc attcgcctat ctccttatag ttaatttcat
     5821 attgataaga acccattttt tcattatctt gatataattc ataatgaatc tttagcttgc
     5881 caccgtctcc acttacgaaa tgtaaaatgc gtaatgtttt aacttctagt ggaattcgtc
     5941 cagccgatat atcgtaaaaa gttgttgtcg tttgtccttc aacaaaatgc aaattcatat
     6001 taatgtcgcc tttacgaatc aacttaacgc tatcatcatc cacttttatt gtaacattaa
     6061 ctgttgcatc ctcaatttgt tctacatacc gaataaagtt agattgcctt tgttgccaag
     6121 ttccttcagt agtaaattca aatttttctt tttcgttgtg ctgtttcaac acttgctttg
     6181 tttgaatact tactttttta tccaatttac tactcacctt aatcttcgca taatagaaat
     6241 attatagcat aaaataaggt catcttgtta tgccttctta aagctaaata ggttcaatta
     6301 attaacaatt ccatacttaa tcaacgcttc atatatgtaa gccattttat aaaatacaaa
     6361 caacacctca aaaccaaaaa acacgagatt ttcactatct ttcatgaaaa cctcgtgttt
     6421 gattataatg atagtgttca gagcactacc ttctttatct atatagtcag ttgatatttt
     6481 taaaatttaa taaccattct accagttata gtaccatttt ccatttcttc aaaaatatca
     6541 ttgatttctt ctaattttct taattgaact ttaggtgtta ctttattttc agcagcaaat
     6601 tcaaacgctt cacgtaagtc ttgtcttgta ccaacaagtg aacctactac ttcaatacca
     6661 tcaagcacta atcttgggat atctaagttc attttatcaa caggtaaacc aacggcaaca
     6721 actcttgcac cagcttttac aacatcaacc gcttggttaa atggcgtctt agcaactgaa
     6781 gttaccactg ttgcatctaa tcctttgtta tcagttaatt tcataacttc tgcaactgga
     6841 tcaacatctt tagaattaat aatagcatca gcacctaatt ctttcgcgaa tgctaattta
     6901 tcatcattga tgtcgaaggc aacaatttta gcccccataa cgtttttagc atattgtaaa
     6961 gctaggttac ctaaaccacc tataccaaaa acacctaacc attgtccagg ttttacatta
     7021 cttactttta cagctttata agttgtcaca cctgcgcatg taatagaaga cgctgctgct
     7081 ggatctaatt tttcaggtac tttcacagca tagtctgcag taacaataac ttgttcagcc
     7141 attgcaccat ctactgtata accagcattt ttcacactac ggcaaagtgt ttcacgacct
     7201 gttgtacaat attcacatct tccacagctt tcgaacatcc aagcgataga cacacggtct
     7261 ccaattttta atgattctac atcttccgca acttcgatga ctttaccaat accttcatga
     7321 cctaaagtaa cgcctgtaac atcaccaaaa tcagcattct taacatgtaa atcggtatga
     7381 caaacgccac aatattccgt ttgtaccaac gcttcaccag gttttaaagc tcttaacttt
     7441 ttgtcctcaa tacttacttt gtgatctttc gttacaactg ctgctctcat aataatgtcc
     7501 tccttatagt cactccagta tgtatgatat taattggcta ttactttcaa aaaagaaaac
     7561 gaataattat agatgtaatt tttatttgta atagacgcca gattaaaaca tactactgtg
     7621 accttgtttt tagttagata tcaaccgtta acttttaacg tttccctagt taatatgtta
     7681 aaagaaatga cataatcgat atgctaacgt gtaattttta cttcacatat atttatttaa
     7741 agataat
//
LOCUS       DAKTIO010000127         7646 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.127, whole genome shotgun sequence.
ACCESSION   DAKTIO010000127 DAKTIO010000000
VERSION     DAKTIO010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7646
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.127"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..110
                     /locus_tag="O0293_002054"
     CDS             <1..110
                     /locus_tag="O0293_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280931.1"
                     /translation="DGNNQIDVCFFVLVNNYYTMSIYPRIINSNGDYAT"
     gene            139..552
                     /locus_tag="O0293_002055"
     CDS             139..552
                     /locus_tag="O0293_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280932.1"
                     /translation="MKPTKVILKDASYLHSKTSITFILKDVVIEEDNKIYYFDTSATF
                     EDQEVKFEFALFDSDMDNLKHMDCDNPVTEIYFIEPDLHFTIIDFNQELLCIYIDFDS
                     GLRHSNMATESGISLRINVAKSDFTKFINELASLH"
     gene            complement(1042..2028)
                     /locus_tag="O0293_002056"
     CDS             complement(1042..2028)
                     /locus_tag="O0293_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCY0280933.1"
                     /translation="MNDQWYKHLIGARTIKTGIAIFLTAVFCMALDLTPIYAILTAVV
                     TIEPTAKASLIKGYRRLPATVIGAGFAVLFTYLFGDQSPFTYALSATFTILFCTKLKL
                     QVGTNVAVLTSLAMIPGIHDAYIFNFLSRTLTAIIGLVTSGLINFMVFPPKYYGQVEE
                     KLSKTDALMYKLFYNRCQELILSRLQSDKSEKAYKNIFNLNNQVETLISYQRDELSYH
                     KKKECDWKLLNQLTKRAYTNRLFITHLSNIIYLPKNTRVNFSGDEKMALLKISSSIKD
                     IFYDGSFKREDDSVETLRSTIKALEISGENQIKSHILYEVLMIYRLLDSRYA"
     gene            2556..3314
                     /gene="map"
                     /locus_tag="O0293_002057"
     CDS             2556..3314
                     /gene="map"
                     /locus_tag="O0293_002057"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCY0280934.1"
                     /translation="MIVKTEEELQALKEIGYICAKVRNTMQAATKPGITTKELDNIAK
                     ELFEEYGAISAPIHDENFPGQTCISVNEEVAHGIPSKRVIREGDLVNIDVSALKNGYY
                     ADTGISFVVGESDDPMKQKVCDVATMAFENAIAKVKPGTKLSNIGKAVHNTARQNDLK
                     VIKNLTGHGVGLSLHEAPAHVLNYFDPKDKTLLTEGMVLAIEPFISSNASFVTEGKNE
                     WAFETSDKSFVAQIEHTVIVTKDGPILTTKIEEE"
     gene            3531..3917
                     /locus_tag="O0293_002058"
     CDS             3531..3917
                     /locus_tag="O0293_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280935.1"
                     /translation="MNYVERYIEQFLRATVRNNIKHYLLMLDEKMKNLDDYMRYLITK
                     KEQLSKLIDSLMLTLENKYIDIAEAFQIQCAREINNQEIENIKSELNKVEAYYAQIET
                     QIQQTSTEKIATEKTSYLINYMNAVA"
     gene            3932..4633
                     /locus_tag="O0293_002059"
     CDS             3932..4633
                     /locus_tag="O0293_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HCY0280936.1"
                     /translation="MTHKYISTQMLIIFTALMIIANFYYIFFEKIGFLLVLLLGCVLV
                     YVGYLYFHKIRGLLAFWIGALLIAFTLLSNKYTIIILFVFLLLLIVRYLIHKFKPKKV
                     VATDEVMTSPSFIKQKWFGEQRTPVYVYKWEDVQIQHGIGDLHIDLTKAANIKENNTI
                     VVRHILGKVQVILPVNYNINLHVAAFYGSTYVNEKSYKVENNNIHIEEMMKPDNYTVN
                     IYVSTFIGDVEVIYR"
     gene            4630..5673
                     /locus_tag="O0293_002060"
     CDS             4630..5673
                     /locus_tag="O0293_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCY0280937.1"
                     /translation="MNHYIRTIGSMLILVYSMLAAFLFIDKVFVNIIYFQGMFYTQIF
                     GIPVFLFLNLIIILLCIIVGSVLAYKINQQNDWIKTQIERSMEGETVGINDQNIEIYS
                     ETLDLYHTLVPLNQELHKLRLKTQNLTNENYNINDVKVKKIIEDERQRLARELHDSVS
                     QQLFAASMMLSAIKETKLEPPLDQQIPILEKMVQDSQLEMRALLLHLRPLGLKDKSLG
                     EGIKDLVIDLQKKVPMKVVHEIQDFKVPKGIEDHLFRITQEAISNTLRHSNGTKVTVE
                     LFNKDDYLLLRIQDNGKGFNVDEKLEQSYGLKNMRERALEIGATFHIVSLPDSGTRIE
                     VKAPLNKEDSYDD"
     gene            5663..6292
                     /gene="vraR"
                     /locus_tag="O0293_002061"
     CDS             5663..6292
                     /gene="vraR"
                     /locus_tag="O0293_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator VraR"
                     /protein_id="HCY0280938.1"
                     /translation="MTIKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKA
                     HELKPDLILMDLLMDDMDGVEATTQIKKDLPQIKVLMLTSFIEDKEVYRALDAGVDSY
                     ILKTTSAKDIADAVRKTSRGESVFEPEVLVKMRNRMKKRAELYEMLTEREMEILLLIA
                     KGYSNQEIASASHITIKTVKTHVSNILSKLEVQDRTQAVIYAFQHNLIQ"
     gene            complement(6408..7625)
                     /locus_tag="O0293_002062"
     CDS             complement(6408..7625)
                     /locus_tag="O0293_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HCY0280939.1"
                     /translation="MSKKDHSSSKYLNSVKEAQEESKKKNKSNPKIDVDRTYIEPQQF
                     QSKKPKKDDQVFFLSRLNKPAKYKKDSNFLSYLIYRIGKDDASGLAAQMTYHFVLAMF
                     PMLLFLLTLLPFFNIKQSQITNMLSNAPAETSTLIKSVIGDITQNSSGGLLSIGLILA
                     IWSASNGMTAIMNSFNVAYDVEDSRNGIVLKLLSVVFTVVMGVVFVVALALPTLGSVI
                     SHFLFGPLGFDEQVKWIFNLIRIVLPIIIIFIIFIVLYSVAPNVKTKLKSVLPGAVFT
                     SIIWLAGSFGFGWYISNFGNYSKTYGSIAGIIILLLWLYITSFIIIVGAEINAIIHQR
                     SVIKGKTPEEAALEHDDNNKNHYNENTRYEYDEDSNATHQRTYHVDEHPSDTYPEDDK
                     NIKDKIVDKLKKD"
BASE COUNT         2719 a         1125 c         1345 g         2457 t
ORIGIN      
        1 tggatggaaa caaccaaatt gatgtgtgtt tttttgttct agtgaataat tattatacaa
       61 tgagtatcta tcctagaatt atcaatagta atggtgatta tgcaacatga aaaaatgaat
      121 gatgaaagga atttgacgat gaagcctact aaagtgatat taaaagatgc atcttattta
      181 catagcaaaa catcgataac atttatttta aaagatgtag ttatcgaaga agataataaa
      241 atttattatt tcgacactag tgcaactttc gaagatcaag aagttaaatt tgaatttgca
      301 ctctttgata gtgacatgga caatttgaaa catatggatt gcgacaatcc tgttactgaa
      361 atatatttta tagaacctga tttacatttt acaattattg attttaatca agaactgctt
      421 tgtatttata ttgattttga ttctggttta aggcattcaa acatggcaac agaatctggt
      481 atttcattaa ggataaatgt tgctaaatca gattttacta aatttattaa tgaattagcc
      541 tctttacatt aatgatttaa atctgatatg taattacaat caaaaaagac agccacatcc
      601 ctccgtagtt taggcgtgtg gctatatttg agtctgaata tttatgcttg taattttaaa
      661 aagggacatg ctatatacga taaaaagagg cggggacata aatcaatgtt ctatgctcta
      721 cgaagttata ttggcagtag ttgactgaac gaaaatgcgc ttgtaacaag cttttttcaa
      781 ttctagtcag gggccccaac aaagagaaat tggattccca atttctacag acaatgcaag
      841 ttggggtgtg ggccccaaca aagagaaatt ggattcccaa tttctacaga caatgcaagt
      901 tggggtgtgg gccccaacac agagaatttc gaaaagaaat tctacaggca aagcgagttg
      961 gggtgggacg acgaaataaa ttttatgaaa atatcatttc tgtcccactc ccatggtgcc
     1021 aattagcata aggtacttaa attaagcata tctgctgtct agcagtcgat aaatcattag
     1081 aacttcgtat agtatatgac ttttaatttg attttcacca ctaatttcaa gtgcttttat
     1141 agtcgaacgt aaagtttcta cagaatcatc ttctctctta aaagaaccat cataaaatat
     1201 atctttgatg ctactactaa tttttagcaa tgccattttt tcgtcacctg aaaagttaac
     1261 acgagtattt ttaggcaagt aaatgatatt tgataaatga gtgataaaca aacgattcgt
     1321 atatgcacgt ttagttaatt gattgagtaa tttccaatca cattcttttt tcttatgata
     1381 gcttaattca tcacgttgat aacttattaa cgtttcaact tgattattta aattgaaaat
     1441 atttttatat gctttttcgc ttttatcaga ttgcagtctt gataagataa gttcttggca
     1501 gcgattgtaa aataatttat acatcaaggc atctgtctta cttaattttt cttcgacctg
     1561 accataatac ttaggtggaa acaccatgaa gttaattaaa cctgatgtca cgagtccaat
     1621 aattgctgtc aatgttcgag acaaaaagtt gaatatgtag gcatcatgaa tacctggaat
     1681 catagctaat gatgttagta cagcgacatt cgtaccaact tgcaatttga gttttgtaca
     1741 gaataaaatc gtgaacgttg cactcaatgc atatgtaaaa ggtgattgat cgccgaataa
     1801 atatgtaaat aatactgcaa agcctgcacc aattaccgta gcaggtaatc tacgataacc
     1861 tttaataagt gatgccttgg cagttggttc aattgtgact acagctgtta aaatggcata
     1921 gatgggtgtt aaatctagtg ccatacaaaa gacagctgtt aaaaaaatgg caataccagt
     1981 tttaattgtt ctggcaccaa ttaaatgttt ataccattga tcgttcattt tttaacctct
     2041 aatcatcgta aaatcttagc gagcgcttta taataatagt atcgtacatt ggaaaagttc
     2101 atgtatgtaa aatatttgaa ataatcatac ataagcatta ctttgatttt catatacatt
     2161 aatcaaagtt ctgggctcca caatgttata ttgttagtag ttgactgaaa taaaatgcac
     2221 ttgtaacaag catttttcaa ttatagtccg gggccccaac atagagaatt tcaaaaaaga
     2281 aattctacag gcaatgcagg ttggcggggc cccaacacag aagctgacga aaagtcagct
     2341 tacgataatg tgcaggttgg cggggcccca acatagagaa attggatcta caatttctac
     2401 aggcaatgca agttggggta caacgataaa gaaatatttt ttctttatca cactatgtct
     2461 cactcacttt ccaaaatact aaagtaacat ctttagtata tcaaagaatt tttgctataa
     2521 taagttataa ttatataaaa aaggaacggg ataaaatgat tgtaaaaaca gaagaagaat
     2581 tacaagcgtt aaaagaaatt ggatacatat gcgctaaagt gcgcaataca atgcaagctg
     2641 caaccaaacc aggtatcact acgaaagagc ttgataatat tgcgaaagag ttatttgaag
     2701 aatacggtgc tatttctgcg ccaattcatg atgaaaattt tcctggtcaa acgtgtatta
     2761 gtgtcaatga agaggtggca catgggattc caagtaagcg tgtcattcgt gaaggagatt
     2821 tagtaaatat tgatgtatcg gctttgaaga atggctatta tgcagataca ggcatttcat
     2881 ttgtcgttgg agaatcagat gatccaatga aacaaaaagt atgtgacgta gcaacgatgg
     2941 catttgagaa tgcaattgca aaagtaaaac cgggtactaa gttaagtaac attggtaaag
     3001 cggtgcataa tacagctaga caaaatgatt tgaaagtcat taaaaactta acaggtcatg
     3061 gtgttggttt atcattacat gaagcaccag cacatgtact taattacttt gatccaaaag
     3121 acaaaacatt attaactgaa ggtatggtat tagctattga accgtttatc tcatcaaatg
     3181 catcatttgt tacagaaggt aaaaatgaat gggcttttga aacgagcgat aaaagttttg
     3241 ttgctcaaat tgagcatacg gttatcgtga ctaaggatgg tccgatttta acgacaaaga
     3301 ttgaagaaga atagttcaac atatactaag actaaagtat gaacatcatt tagttccgga
     3361 gcctattcat attggtttcg gaactgtttt ataataatta agaacacaat caatgcgtca
     3421 tttcaaaaat atgttgtaac aaagtagttt ttaagcaaac atatcatcga catcaacgaa
     3481 gatacatagc gcatttggta ttttaaaact tattataaaa ggtgatagtt atgaactatg
     3541 ttgaacgtta tattgaacag tttttgagag caacagtaag aaataatatc aagcactacc
     3601 ttttaatgct agatgaaaaa atgaaaaatt tagatgatta tatgcgttat ttaattacta
     3661 aaaaagaaca acttagcaag ttaattgaca gtctaatgct aacattagaa aataaatata
     3721 ttgatattgc tgaagcattt caaattcaat gtgcaagaga aatcaataat caagaaattg
     3781 aaaatattaa atcagagttg aataaagttg aagcatatta tgcacaaatt gaaactcaaa
     3841 ttcaacaaac ttcaactgaa aaaatagcaa cagaaaaaac atcgtatcta ataaattata
     3901 tgaacgctgt ggcatagaaa ggcggcgaaa catgacacac aaatatatat caacgcaaat
     3961 gttgatcatt tttactgcat taatgattat tgccaatttt tactacatat tttttgaaaa
     4021 aattggcttt ttactcgttc tattattggg atgtgtatta gtttatgtag gatatcttta
     4081 ttttcataaa atacgtggcc ttttggcgtt ttggataggc gcgctattaa ttgcattcac
     4141 attattgtct aataagtata caatcatcat cttgttcgtc tttttattat tacttattgt
     4201 gcgttattta atacacaagt ttaaaccaaa aaaagtagtt gcgacggatg aggttatgac
     4261 ttcaccatct tttattaaac aaaagtggtt tggtgagcaa cgtacaccag tttatgtata
     4321 taagtgggaa gatgtacaaa ttcaacatgg aattggcgac ctacatattg acttaacaaa
     4381 agctgcaaat attaaggaaa ataataccat tgttgttaga cacattttag gtaaagtgca
     4441 ggttatattg ccggttaatt acaatattaa tttacatgta gctgcttttt atggaagtac
     4501 ttacgtgaat gaaaaatcat ataaagttga aaataacaat attcatattg aagaaatgat
     4561 gaaaccggat aactatacag ttaatatcta cgtatcaacg tttatcggag acgtagaggt
     4621 gatttatcga tgaaccacta cattagaaca attggttcaa tgctcatctt agtatatagc
     4681 atgctagctg catttctgtt catcgataaa gtttttgtaa atatcatcta ttttcaaggt
     4741 atgttttata cacaaatatt cggaatacca gtttttttat ttttaaatct catcatcata
     4801 ttattgtgta ttattgttgg ttcggtactc gcttacaaaa tcaatcagca aaatgattgg
     4861 attaagacgc aaattgagcg ttcaatggaa ggcgaaacag ttggcattaa tgatcaaaat
     4921 atagaaatat atagtgaaac gttagattta taccatacac tcgtaccttt aaatcaagaa
     4981 ttgcataagt tgcgacttaa aactcaaaac ttaaccaatg aaaattataa tattaatgat
     5041 gtgaaagtta aaaagattat tgaagatgaa cgtcaaagac tagcacgaga acttcacgat
     5101 tctgttagtc agcaactttt tgcggcaagt atgatgctat ctgctatcaa agaaacgaag
     5161 ttagaaccac cattagacca acaaattcct attttagaga aaatggttca agattcgcag
     5221 ttagaaatgc gtgctttgct gttacattta agaccgcttg gtttaaaaga caaatcttta
     5281 ggtgagggta ttaaagattt agttattgat ttacaaaaaa aagtgccaat gaaagttgtg
     5341 catgaaatac aagattttaa agtgcctaaa ggtattgaag atcatttgtt cagaattaca
     5401 caggaagcaa tttcgaatac attgcgtcat tcaaacggta caaaagtgac agtagaattg
     5461 tttaataaag acgattattt attgttgaga attcaagata atggtaaagg ttttaatgtt
     5521 gatgaaaaat tagaacaaag ttatggactt aaaaatatgc gtgaaagagc tttggaaatt
     5581 ggtgcaacgt tccatattgt atcattgcca gattcaggta cacgtatcga ggtgaaagca
     5641 cctttaaata aggaggattc gtatgacgat taaagtattg tttgtggatg atcatgaaat
     5701 ggtacgtata ggaatttcaa gttatctatc aacgcaaagt gatattgaag tagttggtga
     5761 aggcgcttct ggtaaagaag caattgccaa agcccatgag ttgaagccag atttaatttt
     5821 aatggattta cttatggatg acatggatgg tgtagaagcg acgactcaga ttaaaaaaga
     5881 tttaccgcaa attaaagtat taatgttaac tagttttatt gaagataaag aggtatatcg
     5941 tgcattagat gcaggtgtcg atagttacat tttaaaaaca acaagtgcaa aagatatcgc
     6001 cgatgcagtt cgtaaaactt ctagaggaga atctgttttt gaaccggaag ttttagtgaa
     6061 aatgcgtaac cgtatgaaaa agcgcgcaga gttatatgaa atgcttacag aacgagaaat
     6121 ggaaatatta ttattgattg cgaaaggtta ctcaaatcaa gaaattgcta gtgcatcgca
     6181 tattactatt aaaacggtta agacacatgt gagtaacatt ttaagtaagt tagaagtgca
     6241 agatagaaca caagctgtta tctatgcatt ccaacataat ttaattcaat agttcatatc
     6301 gaattaagaa aagttactta cgccaatcac aatataacat caaatagaca ccaaaaaaca
     6361 gaggttttcg caataatcat tagcgaaaac ctctgttttg ttggtgttta gtctttttta
     6421 agcttatcaa cgattttgtc ttttatattt ttatcatctt ctggataggt atcactagga
     6481 tgttcatcaa catgatacgt acgttgatgt gttgcattac tatcttcatc atattcgtat
     6541 ctagtgttct cattataatg atttttatta ttgtcatcat gttctaatgc tgcttcttca
     6601 ggtgttttac ctttaattac actacgctga tgaatgattg cattgatttc agcaccgaca
     6661 attataataa aacttgtgat atataaccat agtaacaaaa tgatgatacc cgcgatactg
     6721 ccatatgttt tagaatagtt accaaaattt gaaatatacc aaccaaaacc aaatgaacca
     6781 gctaaccaaa taattgaagt aaatactgca cctggtaata ctgacttaag cttcgtttta
     6841 acgttaggtg caaccgaata taacacgata aatatgataa atataataat gattggtaac
     6901 acaattctaa taaggttaaa aatccatttc acttgttcgt caaatccaag tggaccgaat
     6961 aggaaatgac taattacaga accaagcgtt ggtaatgcta gagcaactac aaacacaacg
     7021 cccataacta cagtgaagac aacacttagt agttttaata cgattccatt acggctatct
     7081 tctacatcgt aagcaacatt gaaagaattc ataattgcag tcattccatt tgaagctgac
     7141 caaattgcta aaatcaaacc gatagataat aagccaccac tggagttttg agttatatca
     7201 ccaattacac tcttaattag agtagatgtt tcagcgggtg cattgcttaa catattagta
     7261 atttgactct gcttaatatt gaaaaatggt aataatgtta ataggaaaag caacataggg
     7321 aacatagcaa gtacgaaatg gtaagtcatt tgtgctgcta gtcctgaggc atcatctttt
     7381 cctatgcgat agatgagata tgataagaaa ttagagtctt tcttatattt tgcaggttta
     7441 tttaatcttg ataagaagaa aacctgatca tcttttttag gtttcttaga ttggaattgt
     7501 tgaggttcaa tatatgtacg atcaacatca attttgggat tacttttatt tttctttttt
     7561 gactcctctt gcgcttcctt aacagaatta aggtattttg aagaagagtg atcctttttc
     7621 gacatgtgca ctccttaacc tttctc
//
LOCUS       DAKTIO010000128         7499 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.128, whole genome shotgun sequence.
ACCESSION   DAKTIO010000128 DAKTIO010000000
VERSION     DAKTIO010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7499
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.128"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..1189
                     /locus_tag="O0293_002063"
     CDS             <1..1189
                     /locus_tag="O0293_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HCY0280940.1"
                     /translation="FAYFKPHLLYLLIGTAIIFVICKLIAQFLIRFKTKDLAEMIETQ
                     GFHNRTVIKKTTEYIPIDYKQTEYDYYPTMFYKRGKKSFVIHIKKDGSRFQDNYLELE
                     TIIEPMFNSELVEKKHIGRYLRYEFMPLKYKKRIVMNGEQESNTIFYDTKIAITHQIT
                     WDFVKAPHGLVTGITGGGKTYFLFYVIRELFRRHSEVRLLDPKVSDLSFMKRVIGDDK
                     VADTKGQILKQLREANNEMEERFRLMNDSSDYKIGNDFRNFDMRPYFIIFDEVTAFTS
                     TLDKKELQEMNDYLINIIMKGRQAGVFMFLTAQRPDADVIKGNVRDQLGLRVSLGNLS
                     NDGYRMTFGQTDKEFQTIHDSDIGRGYISILGQYNEPILFDAPLMEQYDFVEDVKQIL
                     NKE"
     gene            1194..3041
                     /locus_tag="O0293_002064"
     CDS             1194..3041
                     /locus_tag="O0293_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280941.1"
                     /translation="MKHKSVYLLCFLILPILLLTSVSAYAVSNPVGEPSHAVKPKIEK
                     YDLSHYRSIYSEKGDWNPFGQEEISRQINNVSDFFFSMTKILAGVTDYAIENLFQLDV
                     INDFADKIGDFVGDIYQKLLSNLALTLFIIVCFNAFIIFSVQGNAREALKRAFLIMCL
                     IGFGVGILANAGNIIRGTNNIGKDLNNIIMNSTSSIDGNIDYIHENSGMNHIRNQLFD
                     MTIYRTYLVMNYGTVDEKEIKAKGKDRIDNILKLDYDKKSEKELDDIVKDEVEKKNNK
                     YMTQGYVFQKLAISVIGFIITLFMSIVFLSISFAKLIFSTFALFLFLFLVFSWIVSFI
                     PGFELSVFSAFAKTLGYIILSACMTFLFVIVGLCIKLANSFIEPDSQNAYFLNSIFII
                     VILFVMYKKRAQIINFVSRGNISFSPSAIGAGVMNRTQERFNKIRHEQAQNKKAKREN
                     QRDEPAPPLQNDNDLRRRQQDKPMPLFINKDNQKNGNKRREQQESMNGNDVKSASVES
                     NANNYSKQPQKASQQEHQVRETRQRKDIQRSPQVVNQPLNNENHSINRKEQKSVQTAY
                     DTDVQKRQIQNATQNQQSRQSGNRNQPITRNSQSKDRLKEQKDINKHGK"
     gene            3031..4077
                     /locus_tag="O0293_002065"
     CDS             3031..4077
                     /locus_tag="O0293_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0280942.1"
                     /translation="MVNKKDIAKKVIGKTPIGVKLKLAKVIIILCVVAFFIFPVIIMF
                     LLAPDLNKGKDDAGCTVSGGNVSANGIDKFNENAKGGKLEGKGKEIQKIAEKNKVPVN
                     IFMAIIASESQWGKGENATRQNNPLSVMGSKSIHDSTYPTIEDGLNAGAKNLYDVYIS
                     KGLDTPKKIGPKYAPVGASNDPNNMNARWIPTVEKIMKDLGGSEAKTSCSNGKGKSIK
                     FNGKLPHWSNDDPGKGNLYTAGQCTWYAYGMRQKMGKPVSTYWHDAHKWNDRAKAEGY
                     KVDKNPEPGALFIAEQGAGGHDGHYGHVAVVIGVSDGGKTFRITEMNWEGAFKVNERT
                     LKMTDGYSFIHDKE"
     gene            4084..4674
                     /locus_tag="O0293_002066"
     CDS             4084..4674
                     /locus_tag="O0293_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280943.1"
                     /translation="MKQKIIMFTLVTVILFCAVLIGYQIPKQQVKMKQNQIEDLQEEQ
                     RILRDKNGELNKLVKRQSKTVISDEEKQIREVSSNFVKQMFEMKKDSSFKSKAPQIKP
                     LVTKDYYDTLFKDSKDKYDLYDDITVNDIHVYFDTYDPKKDSYKVFVQFDERIETDGD
                     DKIEHRQTSAQLDLVRTAEGWRIDNLKRFNLKPLGR"
     gene            4730..5086
                     /locus_tag="O0293_002067"
     CDS             4730..5086
                     /locus_tag="O0293_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystatin-like fold lipoprotein"
                     /protein_id="HCY0280944.1"
                     /translation="MRRWFVLILGLVILLSACGQKYDKEIDEVTKLEKESIQDVKNTK
                     KYKNVERSKSYYKIYNDGEVIIMTYMPFKDSNTKVSRVYKINQTSDKYEEDSNIDAEK
                     FEKDNKPVYEENNMKK"
     gene            5195..5698
                     /locus_tag="O0293_002068"
     CDS             5195..5698
                     /locus_tag="O0293_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCY0280945.1"
                     /translation="MKKRKKRKELDYKTRLAIAKYLNDGLNIIQISKILGRDDAVLYR
                     EVNKRSVDGVYNPLLAEEDAIKSRSISDEQVAKRRRLNDETKAYIEEKLSLKWSPRQI
                     ASQIEKDTGYYISYPTIYRYIRNGLVKVDVKRDMRQAGKKYNKSSEKRGKLDVGSRVI
                     KYRLKKY"
     gene            5679..6215
                     /locus_tag="O0293_002069"
     CDS             5679..6215
                     /locus_tag="O0293_002069"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCY0280946.1"
                     /translation="MGSKSINNREEFGHYEIDTVWSVRPSTYCLLTIIERKTRYLYAS
                     RLNTRKSNVVCNEIINIMKPLLPKSITMDRGKEFALFEEIEDELNCSIYFSDLGCPYQ
                     RGSIENANGLLRQYYPKGTDFANITQKSLDEAVKQINARPRMIFDYKSSEEMLKYHVS
                     TQNCEPILNDCVRHEPVN"
     gene            6435..6983
                     /locus_tag="O0293_002070"
                     /pseudo
     CDS             6435..6983
                     /locus_tag="O0293_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
BASE COUNT         2754 a          883 c         1336 g         2526 t
ORIGIN      
        1 gtttgcatat tttaaaccgc atttattgta tttattaatt ggtacagcaa ttatatttgt
       61 gatatgtaag cttattgctc agtttttaat acgttttaaa acaaaagatt tggctgaaat
      121 gattgaaaca caaggttttc ataatcgtac agtaatcaag aaaacaactg agtatatccc
      181 tattgattat aaacaaactg aatatgatta ttatccaaca atgttttata agcgtggtaa
      241 aaaatctttt gttattcata ttaagaaaga cggttcgagg tttcaagata attatttaga
      301 gttagagacg attattgaac caatgtttaa ttctgaatta gtagaaaaaa agcatattgg
      361 tcgttatttg agatatgaat ttatgccact taaatataaa aagcgtattg ttatgaatgg
      421 cgaacaagaa tcaaatacaa ttttttatga tacaaaaatt gctattacgc atcaaattac
      481 ttgggatttt gtgaaagcac ctcatggatt ggttacaggt ataaccggag gtggtaaaac
      541 atatttttta ttttatgtga ttcgtgagtt attccgtaga cattcagaag tacgtttatt
      601 agacccaaaa gtttcagatt tgagctttat gaagcgcgtt attggtgatg ataaagttgc
      661 agatactaaa ggtcaaatat taaaacaatt gagagaagct aataatgaaa tggaagagcg
      721 ttttagatta atgaatgata gttcggatta taaaatcggg aatgatttcc gtaattttga
      781 tatgcgcccc tatttcatta tttttgatga ggtaacagct tttacttcaa ctttagataa
      841 aaaagaatta caagaaatga atgattattt aattaatatt attatgaaag gtcgtcaagc
      901 tggtgtattt atgtttttaa cagcacaaag accagatgca gatgtgatta aaggtaatgt
      961 gcgtgaccaa ttagggttac gtgtgtcttt aggtaatttg tcaaatgatg gttatcgtat
     1021 gacctttggt caaactgata aagaatttca aacgattcat gatagtgata tcggccgagg
     1081 ttatatttct atacttggac aatataatga accaatttta tttgatgcac ctttaatgga
     1141 acaatatgat tttgtggaag atgtgaaaca aatattaaat aaggagtgat ttgatgaagc
     1201 ataaaagtgt gtatctttta tgctttttaa ttttgcctat tctgctttta acatctgtga
     1261 gtgcttatgc tgtttctaat ccagttggag agccctcaca tgcagttaag ccaaaaattg
     1321 aaaagtatga tttatcacat tatcgttcaa tatatagtga aaaaggcgac tggaatccgt
     1381 ttggtcaaga agaaatttca cgtcaaatta ataatgtgtc agattttttc ttttctatga
     1441 ctaagatttt agcaggtgtt acagattatg cgattgaaaa tttatttcaa ttagatgtta
     1501 ttaatgattt tgcggataaa atcggcgatt ttgttggcga tatatatcaa aagttattga
     1561 gtaatttagc attaacatta tttattattg tttgttttaa cgcatttatt atttttagtg
     1621 tacaaggtaa tgcaagagaa gcattaaaac gagcgttttt aattatgtgt ttaattggtt
     1681 ttggtgttgg tatacttgcg aatgcaggta atattatacg tggtacaaac aatattggta
     1741 aggacttaaa taatataatt atgaatagta catcttctat tgatggtaat attgattata
     1801 ttcatgagaa ttcaggtatg aatcatatac gtaatcaatt atttgatatg acgatatata
     1861 gaacatattt agtaatgaat tatggaactg tcgacgaaaa agagataaaa gcaaaaggta
     1921 aagaccgtat tgataacatt ttaaaacttg attatgataa aaaatcagaa aaagaattag
     1981 atgatattgt taaagatgag gttgaaaaga aaaataataa atatatgacg caaggttatg
     2041 tatttcaaaa gttggctatt tcagtgattg gttttattat tacacttttt atgtcgatag
     2101 tatttttatc aataagtttt gcgaagttga ttttttctac atttgcttta tttttattct
     2161 tatttttagt attcagttgg atagtgagtt ttataccagg atttgaatta tctgttttct
     2221 ctgcgtttgc aaaaacgtta ggctatatta ttttaagtgc ttgtatgaca ttcttatttg
     2281 tgatagttgg tttgtgtatc aagcttgcaa atagttttat tgaaccggat agtcaaaatg
     2341 cttatttttt aaattcaata tttataattg taatattgtt tgtaatgtat aaaaaacggg
     2401 ctcaaattat caattttgta agtcgtggta atattagttt ttctcctagt gcaattggtg
     2461 ctggtgtgat gaatcgaaca caagaacgtt ttaataagat tcgtcatgaa caagcgcaaa
     2521 ataagaaagc taaacgagaa aatcaaagag atgaacccgc cccaccactt caaaatgata
     2581 atgatttaag aagaagacaa caggataaac caatgccttt atttataaat aaagataatc
     2641 aaaaaaatgg taataagcgc agagaacaac aagagtcaat gaatggtaac gatgtaaaat
     2701 ctgcttctgt tgaatcaaat gcaaataatt attctaaaca accacaaaaa gcatcacagc
     2761 aagaacatca agtacgagaa acaagacagc gaaaagatat acaaaggtcg ccacaagtgg
     2821 ttaatcagcc attaaataat gagaatcatt caataaatcg taaagaacag aagtcagttc
     2881 aaactgctta tgatactgat gttcaaaaac gacaaataca aaatgcgaca cagaatcaac
     2941 aaagtagaca atctggaaat cgtaatcaac ctattacgcg taattctcag tcaaaagacc
     3001 gtttaaaaga gcaaaaggat atcaataagc atggtaaata aaaaagatat tgctaaaaaa
     3061 gtaataggta aaacgccaat aggcgttaaa ttgaaattag cgaaagtcat catcatttta
     3121 tgtgtggtgg ctttttttat atttccagta attataatgt ttttgttagc gcctgattta
     3181 aataaaggta aagatgatgc tggttgtact gttagtggcg gtaatgtttc ggcaaatggt
     3241 attgataagt ttaacgaaaa tgctaaaggt ggaaaattag agggtaaagg aaaagaaata
     3301 cagaaaattg cagaaaaaaa taaagtacct gtgaatattt ttatggccat tattgcgagt
     3361 gaatcacaat ggggtaaagg agaaaatgcg acaagacaaa ataatccttt gtctgttatg
     3421 ggttcaaaat cgattcatga ttcaacatat ccaactattg aagatggtct taatgcggga
     3481 gctaaaaatt tgtatgatgt ttatatttct aaaggtttag atactcctaa aaaaataggt
     3541 cccaaatatg cgcctgttgg tgcgtcaaat gaccctaata atatgaatgc ccgttggata
     3601 ccaacagttg aaaaaattat gaaagattta ggcggttcag aagctaaaac gtcatgtagt
     3661 aatggtaaag gaaagtctat aaagtttaat ggtaaattgc cacattggtc aaatgatgac
     3721 cctggaaaag gtaatttata cactgctggt caatgtactt ggtatgcata cggtatgcgt
     3781 caaaaaatgg gtaaacctgt atcgacttat tggcatgatg cacataaatg gaatgataga
     3841 gctaaagctg agggttataa agttgataaa aatccggaac ctggtgcatt atttattgct
     3901 gaacaaggtg caggcggtca tgatgggcat tatggtcatg tggctgttgt tattggtgta
     3961 agtgatggtg gtaaaacatt tagaataaca gaaatgaatt gggaaggtgc ttttaaagtt
     4021 aatgaacgta cattgaaaat gactgacgga tatagtttta ttcatgataa ggagtgaaaa
     4081 taaatgaagc aaaaaattat tatgtttact cttgtaacag taatattatt ttgtgctgtt
     4141 ttaattggtt atcagattcc taaacaacaa gttaaaatga aacaaaatca aattgaagat
     4201 ttgcaggaag aacaacgtat attaagagat aaaaatggag agttaaataa attagtaaag
     4261 agacaatcaa aaacagttat ttctgatgaa gaaaaacaaa tacgtgaagt ttcttcaaat
     4321 tttgttaaac agatgtttga aatgaaaaaa gattcttctt ttaaatcaaa agcaccacaa
     4381 ataaagcctt tagttacaaa ggattattac gatacattat ttaaggattc taaagataaa
     4441 tatgatttat atgatgatat aacggttaat gatattcatg tttattttga tacttatgac
     4501 cctaaaaaag atagttataa agtttttgtt cagttcgacg aacgtattga aacagatgga
     4561 gatgataaaa ttgaacatcg tcaaacatct gcacaattgg atttagttcg tacagctgaa
     4621 ggttggcgta ttgataattt aaaacgtttc aatttaaaac ctctaggacg ttagtttgtt
     4681 tcaagacgat agcaatgata taatttacgt aaaaaggagt gaatttatca tgaggcgatg
     4741 gtttgtatta attttaggat tagttatatt attatctgca tgtggtcaga aatatgataa
     4801 agaaattgat gaagttacaa agttagaaaa agaaagtatt caagatgtga aaaatacaaa
     4861 gaagtataaa aatgttgagc ggtcaaaaag ttattataag atttataatg atggtgaggt
     4921 tattataatg acttatatgc cttttaaaga tagtaataca aaagtttcaa gagtatataa
     4981 aataaatcag actagcgata aatatgaaga agattctaat attgatgctg aaaagtttga
     5041 gaaagataat aaaccagttt atgaagaaaa taatatgaaa aaataaagaa tcaactccct
     5101 atacgcttta gcgatggtat cgggagttga tgaacatata tcctcttata acttatttta
     5161 taaataacct ttttaaaagt aaaggaggat atacatgaaa aaaagaaaaa aacgtaaaga
     5221 attagactat aaaacacgtc tagctatcgc aaagtattta aatgatggtt taaacatcat
     5281 tcagatttct aagattttag gtcgtgatga tgcagttctt tatagagaag taaataagcg
     5341 ttctgttgat ggtgtttata atcctttatt agctgaggaa gatgctataa aatcgcgtag
     5401 tatttcagat gaacaagttg ctaaacgtag acgtttgaat gatgaaacaa aagcatatat
     5461 tgaagaaaaa ctttcattaa aatggtctcc tagacagata gcaagtcaaa ttgaaaaaga
     5521 tacagggtat tatataagtt atcctactat atatagatat attcgcaatg gattggtaaa
     5581 agttgatgtt aagcgtgata tgagacaagc aggcaagaaa tataataagt cttctgaaaa
     5641 gcgtggcaaa ttagacgtag ggtctcgtgt tataaagtat cggctcaaaa agtattaata
     5701 atcgtgaaga atttggacat tatgaaattg atactgtttg gagtgttaga ccatcaacat
     5761 attgcttgtt aactattatt gaaagaaaga caagatattt atatgctagt agattaaata
     5821 cacgtaaatc caatgtagtt tgtaatgaga ttataaatat tatgaaacca ttattaccca
     5881 aaagtataac tatggataga ggtaaggagt ttgctctatt tgaagaaata gaagatgaat
     5941 tgaattgtag tatttatttt agtgatttgg gttgtccata tcaacgtggt tctattgaaa
     6001 atgcaaatgg acttttaaga cagtattatc ctaaaggtac ggattttgca aatataacac
     6061 aaaagtcatt agatgaagct gttaaacaaa ttaatgcacg gcctcgaatg atatttgact
     6121 ataaatcgag tgaggaaatg ctaaagtacc atgtaagcac acaaaattgt gaacctatat
     6181 taaatgactg tgtgaggcac gaacccgtca attaatatag gttaatctaa gcgaatggtt
     6241 tacaatgagc gcggaaggac aggacgaata aattttgtat gtaaaatgca taatctaaaa
     6301 agaagcatac tatttaaatc tgtggttttt gacagatatt actagtaaaa tgcttctttt
     6361 tcttcttctg tactctttta gtaatattta ttaaaaaaat ttaagaaatt ctcatttttc
     6421 tattgacaca ctgccattta attaaagcaa ttggagtatt gttactcatt gtcggcatcg
     6481 tcataatgaa tcaatttaac aagaataatt tattactaac tgatcaaaag tatttactgt
     6541 tttggcttct attaggattt atttttggtt tctttccacc tattcaaacg acaattaata
     6601 gtgctttagc tagtcatact cattcaccag cctttgcatc attagtatca tttacaattg
     6661 ggtcaatagc gctattgatt ttaaccgcta tttttaatcg ttctttaaaa ctaaaaacaa
     6721 gtcatttaaa attcggtaaa ttaaagccta tctattttac tggcggtata cttggcatgg
     6781 cttttgtaac agctaacatt atcttaatgc ctcatatggg tgcagcatta acaacactta
     6841 ttgggatgtt tggccagatt ctaatgggca tattgataga tcactttgga ttatttggtt
     6901 cacctaaaat agcaatgaca tccagaaaaa ctattggtct attatgtatt ttgacaggca
     6961 ttatactttt aagattattt taaattaact tttagcttat cattttaact tgtaattatt
     7021 tttaaaagtg ataagctatt tttttgtggt ctaaaaatct ttagaaaagc gttgatttgg
     7081 gtagtatgct ttgacacaac aaattttaat ttagcaaatt cgatagtcaa ctcattctta
     7141 agacctaaat taatgttatt ttttaataat ttacaccaaa ttaatagcaa aaattatgtt
     7201 attcgtgcta atatttcata gttggttatt caattaatta aaaataagtc aaaatgcaca
     7261 actttttata attcattgag tcgagtttga aaaataaaag tgctttaatg catgatcaat
     7321 tatcgtactt tctattattt gttacccgtt atcaatcgga ataacgtata gacactttaa
     7381 cgtgctatag attggtttta atcactaaat taatgtgttt ttcttatcat taaaactgca
     7441 ctgagaatta ctaaattaaa aaaattataa aaatttttca tttttagtga taaaattct
//
LOCUS       DAKTIO010000129         7433 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.129, whole genome shotgun sequence.
ACCESSION   DAKTIO010000129 DAKTIO010000000
VERSION     DAKTIO010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7433
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.129"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(626..1072)
                     /gene="perR"
                     /locus_tag="O0293_002071"
     CDS             complement(626..1072)
                     /gene="perR"
                     /locus_tag="O0293_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HCY0280947.1"
                     /translation="MSVEIESIEHELEESIASLRQAGVRITPQRQAILRYLISSHTHP
                     TADEIYQALSPDFPNISVATIYNNLRVFKDIGIVKELTYGDSSSRFDFNTHNHYHIIC
                     EQCGKIVDFQYPQLNEIERLAQHMTDFDVTHHRMEIYGVCKECQDK"
     gene            complement(1169..2119)
                     /locus_tag="O0293_002072"
     CDS             complement(1169..2119)
                     /locus_tag="O0293_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCY0280948.1"
                     /translation="MKVVGLNRMREVETELQQRFSDLDFKFYKKASEIPESDLADLDI
                     LVGYDGGINEAFLRRCPNLKWIAWFATGVNTLPLDYIADHGILLTNGKGVQAKQLSEY
                     ILAFILDDYKKMKLSYDNQRQHIYDSKITGKRLSGQTVLFLGTGAIATRTAKLAKAFN
                     MNLIGLSKSGQNKDEFDEIYTIESLESTLPNADIIINALPETQETIHLLKKKHFELMK
                     DEALFINIGRGSIVKEALLIEVLKSKVIRHAYLDVFENEPLKPNHELYELDNVTITAH
                     ITGNDYEAKYDLLDIFKNNLVNFLNKNGLIENEVDAKKGY"
     gene            complement(2125..2580)
                     /gene="bcp"
                     /locus_tag="O0293_002073"
     CDS             complement(2125..2580)
                     /gene="bcp"
                     /locus_tag="O0293_002073"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCY0280949.1"
                     /translation="MLQKGEQFPIFKLENQDGTVITNDTLKGKKAIIYFYPRDNTPTC
                     TTEACDFRDNLEMFNDLDVAVYGISGDSKKKHQNFIEKHGLNFDLLVDEDFKLAKETG
                     VYQLKKSFGKESMGIVRTTFIIDEQGKVLDVIEKVKVKTQIEELKNILG"
     gene            2661..3950
                     /locus_tag="O0293_002074"
     CDS             2661..3950
                     /locus_tag="O0293_002074"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCY0280950.1"
                     /translation="MNFSESERLQQLSNEYILGGVNSPSRSYKAVGGGAPVVMKEGHG
                     AYLYDVDGNKFIDYLQAYGPIITGHAHPHITKAIQEQAAKGVLFGTPTELEIEFSKKL
                     RDAIPSLEKIRFVNSGTEAVMTTIRVARAYTKRNKIIKFAGSYHGHSDLVLVAAGSGP
                     SQLGSPDSAGVPESVAREVITVPFNDINAYKEAIEFWGDEIAAVLVEPIVGNFGMVMP
                     QPGFLEEVNEISHNNGTLVIYDEVITAFRFHYGAAQDLLGVIPDLTAFGKIVGGGLPI
                     GGYGGRQDIMEQVAPLGPAYQAGTMAGNPLSMKAGIALLEVLEQDGVYEKLDSLGQQL
                     EEGLLKLIEKHNITATINRIYGSLTLYFTDEKVTHYDQVEHSDGEAFGKFFKLMLNQG
                     INLAPSKFEAWFLTTEHTEEDIKQTLKAADYAFSQMK"
     gene            4243..5337
                     /locus_tag="O0293_002075"
     CDS             4243..5337
                     /locus_tag="O0293_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCY0280951.1"
                     /translation="MRLGARIFKTGIAIILAMSIASLLPDDVGLKALAGVSAVVAMQP
                     SIYRSFKTVSDQALGNIIGAILSVTMVTIFSDNFIIMGVTVIVLIAILFKFNLAHVAT
                     LASVTALIIMGQHTGSFYITAFYRFVLVMIGVISSSLVNFVFLPPKFETKIYYNSLNI
                     SSDIFMWFKLVLNDTTEFNNIKQDSHNLKQRVEKLEKIYDYYSEERPITKKHIHQQNR
                     KKILFREVVQTTRQAYEVLNKLSRYQNDLYLLNNNFLLQIKLDLDSLTAFHEQILASL
                     SKKARYNVTHVDYELDNPQKKDLLSTFQHELINHPYQTEYSFANVMQIVAAIEEYRHH
                     LEHLDRIRISFFTYHRSDADIEIVEEDFDL"
     gene            complement(5427..5519)
                     /locus_tag="O0293_002076"
     CDS             complement(5427..5519)
                     /locus_tag="O0293_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280952.1"
                     /translation="MFDFSTILKRKFVTGWWGHNIEKAVRDFLN"
     gene            complement(5528..7264)
                     /locus_tag="O0293_002077"
     CDS             complement(5528..7264)
                     /locus_tag="O0293_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAV1866 family putative multidrug efflux ABC
                     transporter"
                     /protein_id="HCY0280953.1"
                     /translation="MIKRYLQFVKPYKYRIFATIIVGIIKFGIPMLIPLLIKYAIDGV
                     INNHALTTDEKVHHLTIAIGIALFIFVIVRPPIEFIRQYLAQWTSNKILYDIRKKLYN
                     HLQALSARFYANNQVGQVISRVINDVEQTKDFILTGLMNIWLDCITIIIALSIMFFLD
                     VKLTLAALFIFPFYILTVYVFFGRLRKLTRERSQALAEVQGFLHERVQGISVVKSFAI
                     EDNEAKNFDKKNTNFLTRALKHTRWNAYSFAAINTVTDIGPIIVIGVGAYLAISGSIT
                     VGTLAAFVGYLELLFGPLRRLVASFTTLTQSFASMDRVFQLIDEDYDIKNGVGAQPIE
                     IKQGRIDIDHVSFQYNDNEAPILKDINLSIEKGETVAFVGMSGGGKSTLINLIPRFYD
                     VTSGQILIDGHNIKDFLTGSLRNQIGLVQQDNILFSDTVKENILLGRPTATDEEVVEA
                     AKMANAHDFIMNLPQGYDTEVGERGVKLSGGQKQRLSIARIFLNNPPILILDEATSAL
                     DLESESIIQEALDVLSKDRTTLIVAHRLSTITHADKIVVIENGHIVETGTHRELIAKQ
                     GAYEHLYSIQNL"
BASE COUNT         2458 a         1229 c         1057 g         2689 t
ORIGIN      
        1 acaagattta attataactc gtaagttttt gtaagtcaag aattattttt aattatttta
       61 tttctaaaaa ataaatttcc taactcaatt aattcaacaa atattatttt attatgtatt
      121 tgttcgaatt tcaatagtca aatttacact ttattaatat tttaaatcaa caacttaaaa
      181 catttgtttt ttaaatatta gtgccgtttg acgactttta tataatatca agttattcta
      241 gtagagtcaa cataaaacaa cacttatttt tgtttaaaaa ttactgttga agggattatt
      301 ttctattcgt aagtactata tgtatttaaa gtgtaattcc ttatgctttt tacttcagtt
      361 ttacaaacaa acttatactc actttatgga tagttattta gtagaccttc gtttgaatat
      421 ttttatgttt atttataaag gatactagtt attagttaat accacttccg tagttagaat
      481 attcttttct tcaatttaaa tatttttcag aataagcata aaaaaaagtt aaacaacttt
      541 catgtcgttt aacttcttaa atattacgct tacttatatg aagtaagcta acgctttttt
      601 aatttgtcat actaccaaag ttaaattatt tatcttggca ttctttacaa actccataaa
      661 tttccattcg atgatgtgtt acgtcaaagt cagtcatatg ctgagctaat ctttcaattt
      721 catttaactg tggatattga aaatcaacaa tcttaccaca ttgttcacat ataatatgat
      781 aatgattatg tgtattaaag tcgaatcgac ttgatgagtc tccatatgtt aattctttta
      841 caattccaat atctttaaac actcttaagt tattatatat tgtcgcaaca cttatatttg
      901 gaaaatcagg tgaaagtgct tgataaattt catcagctgt tggatgagta tgtgaagaaa
      961 ttaaataacg taatattgct tgtctttgag gtgtaattct tacgcctgct tgtcgcaatg
     1021 atgcaattga ttcttctagt tcatgttcaa ttgattctat ttcaacactc atctatatca
     1081 ccatctttct tatataataa ttattattac ttactataaa tataactgta gtattgcgcg
     1141 cgtgtcaata tttacatgat gatttcattt aatagccttt tttagcatca acttcattct
     1201 caattagacc attcttattg agaaaattaa ctagattgtt tttaaaaata tctaataagt
     1261 catactttgc ttcataatca ttaccagtta tatgcgctgt tatagttaca ttatccaatt
     1321 catataattc atgattaggt ttcaaaggtt cattttcaaa cacatctaaa tatgcatgtc
     1381 gaataacttt actttttaat acttctatta agagcgcttc tttaactatg ctacctcgtc
     1441 ctatatttat aaaaagtgct tcatctttca ttaattcaaa atgttttttc tttagtaaat
     1501 gaatcgtttc ttgcgtttct ggtaaagcat ttataataat gtcagcattt ggtaatgtgc
     1561 tttcaagtga ttcgatagta tatatctcgt caaactcatc tttgttctga cctgacttgc
     1621 tcagaccaat taaattcata ttaaaagcct ttgctaactt cgcagttcta gtagcaattg
     1681 cacctgtacc taaaaataaa actgtttgtc ctgataggcg tttaccagtt attttcgaat
     1741 catatatatg ttgtcgttgg ttatcatatg atagtttcat ctttttataa tcatctaaaa
     1801 tgaaagctaa aatgtattca gataattgtt tagcttgaac accttttcca ttagttaaaa
     1861 gtatgccgtg atctgcaata taatctaacg gcaatgtatt tacacccgtt gcaaaccatg
     1921 caatccattt taaattcggg caacgtcgta aaaatgcctc attgataccg ccatcataac
     1981 caactaatat atctaaatca gccaagtcgc tctcaggtat ttctgatgct tttttataaa
     2041 atttaaaatc taaatctgaa aagcgttgtt gtaattcagt ttcaacttca cgcatacgat
     2101 ttaacccaac aactttcata tatgtcaccc caaaatgttt ttaagttctt ctatttgtgt
     2161 tttaacctta accttctcga taacatctaa tactttacct tgttcatcta ttataaaagt
     2221 cgttcttaca atgcccatac tttctttgcc aaatgatttt tttaactgat atacgccagt
     2281 ttctttagct aatttaaaat cttcatctac taataaatcg aaattcaatc cgtgtttctc
     2341 aataaaattt tggtgttttt tctttgaatc accgcttata ccatatactg caacatctaa
     2401 atcattgaac atttctaaat tgtctctaaa gtcacaagct tctgtggtac aagtaggtgt
     2461 attatctcta ggataaaaat atataatcgc ctttttacct tttaatgtat catttgtaat
     2521 gacagttccg tcttgatttt ctaatttaaa tattggaaat tgttctcctt tttgcaacat
     2581 ataatcacct ttttcttcat tataataatt ttatgatacg atattagatg aaaatattaa
     2641 attaaaagag gttgatataa atgaatttta gtgaaagtga acgtttacaa caactttcaa
     2701 acgaatatat tctaggcggt gtcaattccc cttctcgttc ttataaagct gtaggaggcg
     2761 gtgcacctgt tgttatgaaa gaaggacacg gtgcatattt atatgatgtc gatggcaata
     2821 aatttattga ttaccttcaa gcatacggtc caattattac ggggcatgca catcctcata
     2881 ttactaaagc aattcaagaa caagctgcta aaggtgtttt atttggtaca ccgactgaat
     2941 tagaaattga attcagcaaa aaattacgtg atgcaattcc atctcttgag aaaattcgct
     3001 ttgtaaattc tggaacagaa gcagtcatga caacaattcg tgttgcacgt gcatatacta
     3061 aaagaaataa aattataaaa tttgctggat cttatcatgg ccattctgat ttagtattgg
     3121 ttgcagcagg tagcggccca tctcagctcg gttctccaga ctcagctggt gttccagaaa
     3181 gcgtcgcacg tgaagtcatt actgtacctt tcaatgatat taacgcctat aaagaagcaa
     3241 ttgaattttg gggtgatgaa attgccgcag tattagtaga accaattgtt ggtaactttg
     3301 gaatggtaat gcctcaacct ggatttttag aagaggttaa tgaaatttca cataacaatg
     3361 ggaccctagt gatttatgat gaagtaatta ctgcattccg tttccattac ggtgccgctc
     3421 aagatttatt aggtgttatc cctgatttaa ctgcatttgg taaaattgtt ggcggtggtt
     3481 taccaattgg aggctatggt ggacgtcaag atattatgga acaagtagca cctctaggac
     3541 ctgcatatca agctggtaca atggctggta acccgttatc tatgaaagca ggtattgcat
     3601 tactcgaagt actagaacaa gacggtgttt atgaaaaatt agacagctta ggccaacaac
     3661 tagaagaagg tttacttaaa ttaatcgaaa aacataatat cacagctaca attaatcgta
     3721 tttatggatc tttaacattg tactttacag atgaaaaagt cacacattat gatcaagttg
     3781 aacattctga cggcgaagcg ttcggtaaat ttttcaaatt aatgttaaat caaggtatca
     3841 atttagcacc ttctaagttt gaagcttggt tcttaacaac tgaacataca gaagaagata
     3901 ttaaacaaac tttaaaagct gcagactatg cttttagtca aatgaaataa cttttaagga
     3961 agtatatttt taattgaatt aaaataaagc aaatgctcac atagtagatt cattttatat
     4021 cgttaactat taacgtttaa cttgatatgt cactatgtga gtttttattt tacaaattta
     4081 tactatttta atctctaact aaagttttac atcgctatca gtattatgta tgatttattt
     4141 taacaactat aaataagctt gaattttgta actagtaagt gtataacagt aatgaatacg
     4201 aatattagat attcaataaa cttaaaaggg gcgtcattat ttttgagact aggagctcgg
     4261 atttttaaaa ctggtatagc cattatttta gctatgtcta tcgcttcttt actaccggat
     4321 gatgttggtc tgaaagcctt agctggtgtc agtgctgttg ttgcaatgca acctagtatt
     4381 tatcgttcat ttaaaacagt ttctgatcaa gcattgggta acattatagg tgccatacta
     4441 tctgttacaa tggtaacaat ttttagtgat aattttatca ttatgggtgt taccgtcata
     4501 gtacttattg ctattttatt taaatttaat ttagcacatg ttgctacttt ggcgagtgta
     4561 acagcattaa tcattatggg acaacatacc ggttctttct atattaccgc cttttacagg
     4621 tttgtacttg taatgattgg tgtcataagt tcatctctag ttaactttgt gtttctacca
     4681 cctaaatttg aaacaaaaat ttactataat tcattaaata tatcctcaga tatttttatg
     4741 tggtttaaat tagtattaaa tgatacgacg gagtttaata atattaaaca agatagtcat
     4801 aatttaaaac aacgtgtcga aaagttagag aaaatttatg attactatag cgaagaacgt
     4861 cctattacaa aaaaacatat tcaccaacag aatagaaaga aaatactttt cagagaagta
     4921 gttcagacga ctagacaagc ttatgaagta ttaaacaaat tgtcgcgtta tcaaaacgac
     4981 ttatatttac ttaataacaa tttcctatta caaatcaaat tagatttaga ttcattaact
     5041 gcttttcacg agcaaatttt agctagcctt tctaaaaaag cacgttataa tgtgacacat
     5101 gttgattatg agttagataa tccgcagaaa aaagatttat tgtccacatt tcagcatgag
     5161 ttaataaatc atccttatca aacggaatat tcgtttgcca atgttatgca aattgttgca
     5221 gccatcgaag agtatcgaca ccatttagag catttagacc gcataagaat tagctttttc
     5281 acatatcata gatctgatgc agacattgaa attgttgaag aagactttga tttataacat
     5341 taaaacaatt ttatcaatta atgccacaat gagataaact aatattgaat ctcgaaatta
     5401 aatttaaaaa agcagtaaga ttattttcaa ttaagaaaat ctcttactgc tttttctatg
     5461 ttatgacccc accatcctgt tacaaatttt ctcttcaaga ttgtactgaa gtcaaacaac
     5521 taatacttta taagttttga atgctatata aatgctcgta agcaccttgt tttgcaatca
     5581 attcacgatg cgtacctgtt tcaacaatat gtccattttc aattacgaca attttgtcag
     5641 catgtgtaat agtggacaag cgatgcgcta cgataagtgt cgttcgatct ttactcaaca
     5701 catctaatgc ttcttgaata atggattcac tttctaaatc aagtgcactt gttgcttcat
     5761 ccaagataag aattggcgga ttatttaaaa atattctagc aatcgataat ctttgttttt
     5821 gaccacctga taatttaaca cctcgttcac ctacttcagt gtcatatccc tgtggcaagt
     5881 tcataataaa gtcatgtgca ttagccattt tcgccgcttc aactacttct tcatctgttg
     5941 ctgttggacg accaagtaaa atattttcct taactgtatc agagaataaa atattatcct
     6001 gttgcaccaa tcctatttga tttcttaaac ttcccgttaa aaaatcttta atgttgtgac
     6061 catctattaa aatttgccca gaagttacat cgtaaaatct cggtattaag ttaattaatg
     6121 ttgatttacc accaccactc atacctacga aagcaactgt ttctcctttt tcaatactca
     6181 aattaatatc ttttaaaatt ggagcttcgt tatcgttata ttgaaaacta acatgatcaa
     6241 tatcaatacg accttgttta atttcaatag gttgagcacc aacaccattt ttgatgtcat
     6301 aatcttcatc aattaattgg aatacacggt ccattgaagc aaaactttgc gttaaagttg
     6361 taaatgatgc gactaaacga cgtaaaggcc cgaacaataa ctctaagtat ccaacaaatg
     6421 ctgcaagtgt acctactgtg attgatccag aaatagcaag atatgcacca acaccgatga
     6481 caataattgg tccaatatct gtaactgtat taattgcggc aaaggaatag gcattccatc
     6541 ttgtatgttt caacgcacgt gttaggaaat tagtattctt tttatcaaag tttttcgctt
     6601 cattgtcttc aatcgcaaaa cttttaacga ctgaaatacc ttgaacacgt tcatgcaaga
     6661 atccttgaac ctcagctaat gcttgagatc tttcacgtgt caattttctt aatcttccaa
     6721 agaaaacgta caccgttaaa atgtaaaatg ggaagataaa cagtgctgct aaagtcaatt
     6781 tcacatctaa aaagaacatt atggatagtg caataataat tgttatacaa tctaaccaaa
     6841 tattcattaa cccggttaaa atgaaatctt ttgtttgttc aacatcatta atcactctag
     6901 atattacttg acctacttga ttattagcat aaaatctcgc acttaaagct tgtaaatggt
     6961 tgtataactt tttacgtata tcatacaata ttttattact tgtccattgc gccaaatatt
     7021 gacgtataaa ttcaattggt ggtctcacta ttacaaaaat aaataatgcg ataccaatgg
     7081 caatagttaa atgatgaact ttttcatcag tcgttagtgc gtggttatta atcacgccat
     7141 ctattgcata tttaattaat agtggtataa gcattggtat accaaactta attatcccaa
     7201 caataatcgt tgcaaaaata cgatatttgt atggcttaac aaattgcaaa tatcgtttaa
     7261 tcatacaatc ccccctaatc tattgcccta tcctattcat aagcataaaa atgaatagag
     7321 gttggataca taatttgtag atgtaaattc ttcttacaat ttacattttt aaaattaaga
     7381 tatctcaacc tctgtcatat tcatgtgatt aaatacgttg tcttattcgt atg
//
LOCUS       DAKTIO010000130         7409 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.130, whole genome shotgun sequence.
ACCESSION   DAKTIO010000130 DAKTIO010000000
VERSION     DAKTIO010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7409
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.130"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            110..838
                     /locus_tag="O0293_002078"
     CDS             110..838
                     /locus_tag="O0293_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5067 domain-containing protein"
                     /protein_id="HCY0280954.1"
                     /translation="MKKVMGILLASTLILGACGHHQDSAKKESTSHKKKENDNEELNE
                     ELKEFKSKKNMDIKIKGDTIVSDKFEAKIKEPFIINEKDEKKKYIAFKMEITAKKDDK
                     DLNPSSISHDYINITQDDKNTVNKLRDGYLLSDKKYKDWTEHNQDQIKKGKTAQAMFI
                     YELRGDGNINLNVHKYSEDKTVDSKSFKFSKLKTEDFSHRAETREEVEKKEKEFEEEY
                     KKEQEREKEKEKQKDDDHSGLDEV"
     gene            923..1176
                     /locus_tag="O0293_002079"
                     /pseudo
     CDS             923..1176
                     /locus_tag="O0293_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015728980.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1192..1515
                     /locus_tag="O0293_002080"
     CDS             1192..1515
                     /locus_tag="O0293_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280955.1"
                     /translation="MNNEELEMRLLLMKQSIEQLQEELAPNLKTRDLVLLRYMYSYKE
                     INMLDSYLFQLATNKEQITKKQFKTKLENIREVPEIPIRQVNDILEGYKNSELYVELI
                     NSILK"
     gene            complement(2385..2746)
                     /gene="ssrA"
                     /locus_tag="O0293_002081"
     tmRNA           complement(2385..2746)
                     /gene="ssrA"
                     /locus_tag="O0293_002081"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2837..3301)
                     /gene="smpB"
                     /locus_tag="O0293_002082"
     CDS             complement(2837..3301)
                     /gene="smpB"
                     /locus_tag="O0293_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCY0280956.1"
                     /translation="MAKKKSPGTLAENRKARHDYNIEDTIEAGIVLQGTEIKSIRRGS
                     ANLKDSYAQVKNGEMYLNNMHIAPYEEGNRFNHDPLRSRKLLLHKREIIKLGDQTREI
                     GYSIVPLKLYLKHGHCKVLLGVARGKKKYDKRQALKEKAVKRDVARDMKARY"
     gene            complement(3323..5695)
                     /gene="rnr"
                     /locus_tag="O0293_002083"
     CDS             complement(3323..5695)
                     /gene="rnr"
                     /locus_tag="O0293_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCY0280957.1"
                     /translation="MNLKQSIEEIINQPEYEPMSVSDFQDALGLSSADSFRDLIKVLV
                     ELEQSGLIERTKTDRYQKKHSYRGQSKLIKGTLSQNKKGFAFLRPEDEDMEDIFIPPT
                     KINRALDGDTVIVEIHQSKGEHKGKIEGEVKSIEKHSVTQVVGTYSEARHFGFVIPDD
                     KRIMQDIFIPKGQSLGAVDGHKVLVQITKYADGSDNPEGHISAILGHKNDPGVDILSI
                     IYQHGIEIEFPDEVLQEAEAVPDHIENTEIKGRHDLRDELTITIDGADAKDLDDAISV
                     KKLANGNTQLTVSIADVSYYVTEGSALDKEAYDRATSVYLVDRVIPMIPHRLSNGICS
                     LNPNVDRLTLSCRMEIDASGRVVKHEIFDSVIHSDYRMTYDAVNQIITEKDPNIREQY
                     NEITPMLDLAQDLSNRLIQMRKRRGEIDFDISEAKVLVNEDGIPTDVQLRQRGEGERL
                     IESFMLIANETVAEHFSKLDVPFIYRVHEQPKSDRLRQFFDFITNFGIMIKGTGEDIH
                     PTTLQKVQEEVEGRPEQMVISTMMLRSMQQAHYDDVNLGHFGLSAEYYTHFTSPIRRY
                     PDLTVHRLIRKYLIEKSMDNKEVKRWEDKLPELAEHTSKRERRAIEAERDTDELKKAE
                     YMIQHIGDEFEGIVSSVANFGMFIELPNTIEGMVHIANMTDDYYRFEERQMALIGERQ
                     AKVFRIGDTVKVKVTHVDVDERLIDFQIVGMPLPKNDRSQRPARGKTIQAKTRGKSLD
                     KSKSDDKGRKKKGKQRKGKNQRNNDKSGNSKHKPFYKDKSVKKKARRKKK"
     gene            complement(5729..6469)
                     /locus_tag="O0293_002084"
     CDS             complement(5729..6469)
                     /locus_tag="O0293_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HCY0280958.1"
                     /translation="MQIKLPKPFFFEEGKRAVLLLHGFTGNSSDVRQLGRFLQKKGYT
                     SYAPQYEGHAAPPDEILKSSPFVWFKDALDGYDYLVEQGYDEIVVAGLSLGGDFALKL
                     SLNRDVKGIVTMCAPMGGKTEGAIYEGFLEYARNFKKYEGKDQETIDNEMDHFKPTET
                     LKELSEALDTIKEQVDEVLDPILVIQAENDNMIDPQSANYIYDHVDSDDKNIKWYSES
                     GHVITIDKEKEQVFEDIYQFLESLDWSE"
     gene            complement(6585..6818)
                     /gene="secG"
                     /locus_tag="O0293_002085"
     CDS             complement(6585..6818)
                     /gene="secG"
                     /locus_tag="O0293_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCY0280959.1"
                     /translation="MHTFLIVLLIIDCIALITVVLLQEGKSSGLSGAISGGAEQLFGK
                     QKQRGVDLFLNRLTIILSILFFVLMICISYLGM"
     gene            complement(6885..7343)
                     /locus_tag="O0293_002086"
     CDS             complement(6885..7343)
                     /locus_tag="O0293_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280960.1"
                     /translation="MADRTNKEIKTGRFIATASIVFSILLIIHYFVSLDNATAKALLN
                     LTNQNTSDKAIDYILNSFRFTGIMYILAYLAGFITFWNRHTYVWWFMFAVYVSNSLFT
                     LINLSITIQAIKAAHGAYLTLPILIVIIGSVALAIYMLVVSIKRKSTFNR"
BASE COUNT         2564 a         1325 c         1072 g         2448 t
ORIGIN      
        1 aatatcatct tagaatctca caaaatatca actttgttta attactcaaa atgataagtc
       61 atttagagtt tttaaaataa atttttgcga aataaaggag acatgttaaa tgaaaaaagt
      121 aatggggata ttattagcaa gtacacttat cttaggtgct tgtggacatc atcaagatag
      181 tgcaaaaaaa gagagcacta gtcacaaaaa gaaagaaaat gacaatgaag aattaaatga
      241 agaacttaaa gaatttaaaa gcaaaaaaaa tatggatata aaaattaaag gcgatactat
      301 tgttagtgac aaatttgaag ctaaaataaa agaaccgttt atcatcaatg aaaaagatga
      361 gaaaaagaaa tatatcgctt ttaaaatgga aattactgct aaaaaagacg ataaagattt
      421 aaatccatct tctatttctc atgactatat taatatcact caagatgata aaaatacagt
      481 aaataaatta agagatggtt atcttttaag tgataaaaaa tataaagatt ggacagaaca
      541 taaccaagat caaattaaaa aaggcaaaac tgcacaagcc atgtttatct atgagttaag
      601 aggtgatgga aatattaatt taaatgtcca taaatactca gaagataaaa cagttgattc
      661 taaatcattc aaatttagta aacttaaaac cgaagatttt tctcatagag cggaaacaag
      721 agaagaagta gaaaagaaag aaaaagaatt tgaagaagag tacaaaaaag aacaagaacg
      781 agagaaagaa aaagaaaagc aaaaagatga cgaccacagt ggtttagatg aagtataaat
      841 ttggtttgtt gattgtctta cattcagtta tttaattttt agagaaactg acttatttat
      901 agcttagcca acatgatact ataaagctat gattaaaaat aaaaaatctg tgtttaatgg
      961 tgttaaagaa atcagtcccc gtgccggaaa atcataaaaa aaagatggga taaatttgta
     1021 aatcccgcat tgaataatgc attaaaacaa gaagaggcta catggggaaa tgtacaaggt
     1081 caagtttcac aagcattgat gggaactggt attaaagatt ctactgcaag aagcataggg
     1141 ttttgggttt cccaagttgg tcaagcatta atataaaaag agtgtgataa aatgaataat
     1201 gaagaattag aaatgagatt gttattaatg aaacaatcaa tagaacaatt acaagaagaa
     1261 ctagcgccta acttaaaaac tagagattta gtgttattaa gatatatgta ttcatataaa
     1321 gaaattaata tgctagattc ttacttgttc caattggcta caaataaaga gcaaataaca
     1381 aaaaaacaat ttaaaacaaa attggaaaac attagagaag taccagaaat acctattaga
     1441 caagttaatg atatattaga aggttataaa aatagtgagt tatatgttga gttgataaat
     1501 agcatcctaa aataaaaata aacatactaa gattagctat gaaggaatct atgacgatag
     1561 attttttcat agctattttt tatagttata aagaggagta gtctgttctg actcttggat
     1621 tttaaatatg tataataaac aagtaagctt tactctcgcc ttttaaaatt cgtttgtagt
     1681 atgttgggtt cttaaaatcg tgaataggaa aatgcaatga gaaaggttaa gtaaagtttt
     1741 taacttctca tttattcaat ggaaaatttt ttatcgatta cttagacatt aatattagta
     1801 aaagaagtag tgtagttgca aattataaaa actaaaaatt ccaaaaagag tttatcatcc
     1861 aaaacaataa aaatggctac aatggtatgt tgtgaaatgc atttcttaac tacgacttct
     1921 tttatttccg tctcgtcaac tattgctgat tgtgttaata ggcatattga ttctaatgaa
     1981 aatatcaaaa ctcggctcat ctctttataa ctacacccac ttagtgacgt aacaaaaatg
     2041 aaaagttctt tttcagtagc catttatcca attaagtgta gacgattcac atttttatat
     2101 tgacatatat aacatggaga attttttgcg tataaagaag tttcttcatg aacaatgttg
     2161 aatgtaaaac tcagaatcca acttttttga cacttatttg acacacatca ctgtcccctt
     2221 atatctcaaa aactaattca taatcactta ttactctttc ctccatcaaa taaacaccta
     2281 tacaaacact gctatatcaa tactcacaac aaattctgca atgcattatc ccctgaaaaa
     2341 cacaaaataa aaaagcccac aaccacaaag gctgtaggct acaaatatgg agacggcggg
     2401 atttgaaccc gcgtccaaag gtcctgatac acatctttct acgtgtgtag tttatcgata
     2461 ggtttcgcat catgaaaagt gataaacaac caacatgatg ctagtttgat taagtttctt
     2521 ctaaacagac ttcaaacggc agtgtttagc atatcctatt aaggttgaat cgcgttaaca
     2581 gcacatagga aatgctgtta ggcgatgcag agtgcgatta ggcagctact gcgaaattat
     2641 tgtttgattt gccagttatt ataaactgtg tgtgttgatg acgaagacaa ccctccgaca
     2701 cgcttaatga gctcggggga cccctgtcga atccatgaac gtccccgaaa taataaatca
     2761 aacaaatcat acttttgtaa tttacaaaag cacatataac tatatcaaat atagcccgat
     2821 taagcaacta aatcgcttaa taacgggctt tcatatcgcg cgcaacatct cgtttgactg
     2881 ctttttcttt caaagcttga cgtttatcat attttttctt acctcgtgca acaccaagta
     2941 atactttaca atgtccatgc ttcaaataaa gctttaacgg cacaatcgaa taaccaatct
     3001 cacgtgtttg atcacccaat ttaatgattt cacgcttgtg caataataat tttcgagaac
     3061 gaagaggatc gtgattaaaa cgattccctt cttcgtatgg tgctatatgc atattattca
     3121 aatacatttc accgttttta acttgcgcat aactatcttt aaggttagca ctacctcggc
     3181 gaattgattt tatttctgtg ccttgcaata caattcccgc ttcaatcgta tcttcaatat
     3241 tataatcatg tcttgcctta cgattttccg ctaatgtacc tggtgatttc ttcttagcca
     3301 ttcatactca cctcattgct gcttattttt tcttacgacg tgctttcttt ttcacacttt
     3361 tatctttata aaatggctta tgcttactat tacctgattt atcattatta cgttggtttt
     3421 tacctttacg ttgcttacct tttttcttac gacccttatc atcagatttt gatttatcta
     3481 atgatttacc acgcgttttg gcttgaattg tcttacctcg cgctgggcgc tgtgatcgat
     3541 catttttcgg taaaggcata cctacaattt gaaaatcaat taatcgttca tctacatcaa
     3601 catgcgtcac tttaacctta actgtgtcac caattctaaa tactttagct tgacgctcac
     3661 caattaatgc catttgacgc tcttcaaaac ggtaataatc atcagtcata ttcgcaatat
     3721 gaaccatacc ttctatcgta tttggcaatt caatgaacat accgaagtta gctactgagc
     3781 tgacaatacc ttcaaattca tcaccaatat gttgaatcat atattctgct tttttcaatt
     3841 catcagtatc acgttctgcc tcaatagcac gacgttcacg tttagaagta tgttcagcta
     3901 actcaggcaa tttgtcttcc caacgcttca cttctttgtt atccattgat ttctcaatta
     3961 aatacttacg gattaaacga tgaactgtta aatcaggata acgtctaatt ggtgatgtaa
     4021 aatgcgtata atattcagct gataagccaa aatgtcccaa gttcacatca tcataatgcg
     4081 cttgttgcat tgaacgcaac atcattgttg aaatgaccat ttgttcaggt cgaccttcta
     4141 cttcttcttg aaccttttga agtgttgttg gatgaatatc ttcgccagta cccttaatca
     4201 tgatgccaaa gtttgtaata aaatcaaaga attgtcttaa gcgatctgat ttaggttgct
     4261 catgcactcg gtaaataaaa ggtacatcta acttactaaa atgttcagca actgtttcat
     4321 ttgcaattaa cataaatgat tcaattagac gttcaccctc gccacgttgt cttaattgaa
     4381 catctgttgg tataccgtct tcgttaacta atacttttgc ttcactaata tcaaaatcga
     4441 tttcaccacg tcgttttctc atttgaatca aacgattaga taaatcttgt gctaaatcta
     4501 gcataggcgt aatttcatta tattgttcgc gaatgttagg atccttttca gtaataatct
     4561 gatttaccgc atcatacgtc attcgataat cagaatgtat aacactatca aaaatttcat
     4621 gtttaacaac gcgaccacta gcatcgattt ccatgcgaca gcttagagtt aaacgatcaa
     4681 cattaggatt caatgaacaa ataccattac ttaatcgatg tggaatcatt ggaattacac
     4741 ggtcaacaag atatacactt gtcgctctat catatgcctc tttatccaat gcagaacctt
     4801 ctgttacata atagctgaca tcagcaatac ttacagttaa ttgcgtatta ccgttcgcta
     4861 actttttaac actaattgcg tcatctaagt ctttagcatc agcaccatca attgtgattg
     4921 tcaattcatc acgtaaatca tgacggcctt taatttcagt attttcaata tgatcaggta
     4981 ctgcttcagc ttcttgtaac acttcatcag gaaattcaat ttctatgcca tgttgataga
     5041 taatagataa aatatctacg ccaggatcat ttttatgtcc taaaatagca gaaatatgtc
     5101 cttctggatt atctgaacca tcagcatact tagtaatttg tacaagtacc ttatgaccat
     5161 cgactgcgcc taaactttga cctttaggaa tgaaaatatc ttgcataata cgtttatcat
     5221 ccggaataac aaagccaaaa tgtctagctt cactatacgt accaacaact tgagttacag
     5281 aatgcttctc aatcgactta acttcccctt cgattttacc tttatgttca ccttttgatt
     5341 gatggatttc tacaataaca gtatctccat ccaaggcacg attaattttc gtcgggggaa
     5401 taaatatatc ttccatatcc tcatcttcag gtcttaagaa tgcaaagcct tttttatttt
     5461 gacttaacgt tccttttatc aattttgatt gacctctata actatgcttt ttttggtatc
     5521 tgtctgtttt tgtacgttcg attaatcctg attgttctaa ctccacaagc accttaatta
     5581 aatctctaaa cgagtcggca ctgcttaaac ctaatgcatc ttgaaaatct gacactgaca
     5641 taggttcata ttcaggttga ttaataatct cttctataga ttgctttaaa ttcattatgc
     5701 ccctcctttc taatgttaaa atctcttttt attctgacca gtctaatgac tctaaaaatt
     5761 gataaatatc ttcaaatact tgttctttct ctttatcaat cgtaataaca tgtccagatt
     5821 cactgtacca cttgatattt ttgtcatcag aatctacatg gtcatatata taatttgcgg
     5881 attgtggatc aatcatattg tcgttttctg cttgaatcac taaaatagga tccaacactt
     5941 catcaacttg ctctttaatc gtatctaatg cttcacttag ttcttttaaa gtttcagttg
     6001 gtttaaaatg atccatttca ttatcaatag tctcttgatc tttaccttca tactttttaa
     6061 aattgcgtgc atattctaaa aagccttcat aaatggcacc ttcagttttg ccacccatag
     6121 gagcacacat cgttacaata ccctttacat ctctatttaa gcttaatttt aaagcaaaat
     6181 ccccacctaa tgatagacca gcaacaacaa tttcatcata accttgttca acaagataat
     6241 cataaccatc taacgcatct ttaaaccaaa cgaaaggact agatttcagt atttcatctg
     6301 gtggtgccgc gtggccttca tattgcggtg catatgatgt ataacctttc ttttgtaaaa
     6361 atcgacctaa ttgacgaaca tcagacgaat tgcctgtaaa accatgtagt aataacacgg
     6421 cacgtttacc ttcctcaaaa aagaaaggtt ttggtaattt tatctgcata aatctaacgc
     6481 tccctttgca tacttaacta ggctatatat tttacatagc ttattgttaa actattacat
     6541 tcttaattat aaataaaaaa gcccgacatt tacatcgccg gaccttacat accaagataa
     6601 cttatgcaaa tcataagtac aaaaaataat attgataaaa taattgttaa tctatttaag
     6661 aataaatcga cgccacgttg tttttgttta ccgaataact gctcagcacc accactgatg
     6721 gcacctgaaa gtccactgct tttaccttct tggagtagta caacagttat taatgcaata
     6781 caatcaatga ttaataatac gattaaaaat gtatgcataa attgtcctcc gttccttata
     6841 tacgacaagt agtatatcat atttttattg ttaaaatcaa ttttctagcg attaaatgta
     6901 cttttacgtt tgatagaaac aacaagcata taaatcgcta atgcaaccga acctataata
     6961 acaattaaaa ttggcaatgt taagtacgca ccgtgtgcag cttttattgc ttgaattgtg
     7021 attgataaat taatcaacgt aaacaaacta tttgatacat aaactgcaaa cataaaccac
     7081 cacacataag tatgtcgatt ccaaaaagtg atgaagcctg ctagataagc caaaatatac
     7141 ataataccag tgaatctaaa gctgtttaaa atgtaatcaa tcgctttatc tgaagtgttt
     7201 tgattcgtta aattaagtaa tgctttggca gtcgcattat ccaacgaaac aaagtaatga
     7261 ataatcaata atattgagaa tacgattgat gcagttgcaa taaagcgtcc tgttttaatt
     7321 tctttattcg ttctatcagc catttgttta ctcctcatat atttaaatac gttctataaa
     7381 acctttattt aattttacct tttaataaa
//
LOCUS       DAKTIO010000131         7366 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.131, whole genome shotgun sequence.
ACCESSION   DAKTIO010000131 DAKTIO010000000
VERSION     DAKTIO010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7366
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.131"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(237..680)
                     /locus_tag="O0293_002087"
     CDS             complement(237..680)
                     /locus_tag="O0293_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0280961.1"
                     /translation="MSIITRLFNNSDFEKLNQLCKLYDDLGYPTNENDLKKRLKKITN
                     HDDYFLLLLIKENKIIGLSGMCKMMFYEKNAEYMRILAFVIHSEFRKKGYGKRLLADS
                     EEFSKRLNCKAITLNSGNRNERLSAHKLYSDNGYVSNTSGFTKQL"
     gene            complement(845..1222)
                     /locus_tag="O0293_002088"
     CDS             complement(845..1222)
                     /locus_tag="O0293_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280962.1"
                     /translation="MFISLAIWSINSMGNKQCISLNLLLSDDTFAVISVMTTLGTAIL
                     GGIAPSIYGIFTSPIDKKSSFRAISDWEQEQRNNVKDLMMLLPFTLLSILIVIFIFQF
                     AYSKLFNTIAIIIFIIILVVMFF"
     gene            complement(1540..2130)
                     /locus_tag="O0293_002089"
     CDS             complement(1540..2130)
                     /locus_tag="O0293_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCY0280963.1"
                     /translation="MSELTAKQARFVNEYIRTLNVTQSAIKAGYNANSAHVTACRLLK
                     KPHIKQYIQEQKDKIIDENVLTAKELLHVLTNAAVGDETETKEVVVKRGEYKENPQSG
                     KVQLVYNEHVELIEVPIKPSDRLKARDMLGKYHKLFTDKHDINGNVPIFINIGEWDGG
                     DEGLDKAVKDVSNDNPNHTVIVDDIPLEDYESIRFL"
     gene            complement(2127..2339)
                     /locus_tag="O0293_002090"
     CDS             complement(2127..2339)
                     /locus_tag="O0293_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island family protein"
                     /protein_id="HCY0280964.1"
                     /translation="MTDDNVNDHIIKNHIEMIVDRLATDKEFYIFDSLIQGLSYQDIS
                     SALDCSEQSVILWYETILDKIVGVIK"
     gene            2400..2946
                     /locus_tag="O0293_002091"
                     /pseudo
     CDS             2400..2946
                     /locus_tag="O0293_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002476300.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(3041..4657)
                     /gene="groL"
                     /locus_tag="O0293_002092"
     CDS             complement(3041..4657)
                     /gene="groL"
                     /locus_tag="O0293_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500736.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCY0280965.1"
                     /translation="MVKQLKFSEDARQAMLRGVDQLANAVKVTIGPKGRNVVLDKEFT
                     APLITNDGVTIAKEIELEDPYENMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEG
                     LKNVTSGANPVGLRQGIDKAVKVAVEALHENSQKVENKNEIAQVGAISAADEEIGRYI
                     SEAMEKVGNDGVITIEESNGLNTELEVVEGMQFDRGYQSPYMVTDSDKMVAELERPYI
                     LVTDKKISSFQDILPLLEQVVQSNRPILIVADEVEGDALTNIVLNRMRGTFTAVAVKA
                     PGFGDRRKAMLEDLAILTGAQVITDDLGLDLKDASIDMLGTASKVEVTKDNTTVVDGD
                     GDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKL
                     RIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIEAEGDIETGVNIVLKALTAPVRQ
                     IAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNMLEEGIVDPTKVTRSALQHAASVA
                     AMFLTTEAVVASIPEKNNDQPNMGGMPGMM"
     gene            complement(4733..5017)
                     /gene="groES"
                     /locus_tag="O0293_002093"
     CDS             complement(4733..5017)
                     /gene="groES"
                     /locus_tag="O0293_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCY0280966.1"
                     /translation="MLKPIGNRVIIEKKEQEQTTKSGIVLTDSAKEKSNEGVIVAVGT
                     GRLLNDGTRVTPEVKEGDRVVFQQYAGTEVKRDNETYLVLNEEDILAVIE"
     gene            5192..5935
                     /locus_tag="O0293_002094"
     CDS             5192..5935
                     /locus_tag="O0293_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCY0280967.1"
                     /translation="MTRLWASLLTVIIYILSQFLPLLIVKKLPFVQYSGIELTKAVIY
                     IQLVLFLIAATTIILINLKIKNPTKLELEVKEPKKYIIPWALLGFALVMIYQMVVSIV
                     LTQIYGGQQVSPNTEKLIIIARKIPIFIFFVSIIGPLLEEYVFRKVIFGELFNAIKGN
                     RIVAFIIATTVSSLIFALAHNDFKFIPVYFGMGVIFSLAYVWTKRLAVPIIIHMLQNG
                     FVVIFQLLNPEALKKATEQANFIYHIFIP"
     gene            complement(5960..7285)
                     /locus_tag="O0293_002095"
     CDS             complement(5960..7285)
                     /locus_tag="O0293_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280968.1"
                     /translation="MSYHWFKKMLLSTSILILSSSSLGLATHTVEAKDNLNGEKPTTN
                     LNHNITSPSVNSEMNNNETGTPHESNQTGNEGTGSNSRDANPDSNNVKPDSNNQNPST
                     DSKPDPNNQNPSPNPKPDPDNPKPKPDPKPDPDKPKPNPDPKPDPDNPKPNPDPKPDP
                     NKPNPDPKPDPDNPKPNPDPKPDPNKPNPDPKPDPDKPKPNPNPKPDPNKPNPNPSPD
                     PDQPGDSNHSGGSKNGGTWNPNASDGSNQGQWQPNGNQGNSQNPTGNDFVSQRFLALA
                     NGAYKYNPYILNQINKLGKDYGEVTDEDIYNIIRKQNFSGNAYLNGLQQQSNYFRFQY
                     FNPLKSERYYRNLDEQVLALITGEIGSMPDLKKPEDKPDSKQRSFEPHEKDDFTVVKK
                     QEDNKKSASTAYSKSWLAIVCSMMVVFSIMLFLFVKRNKKKNKNESQRR"
BASE COUNT         2255 a         1377 c         1027 g         2707 t
ORIGIN      
        1 tttagacaac gctattattt ttagtttttc aattctatta tgtcataatt atgtcactca
       61 aaaactgttt tccaacattg atttattgtt ggaaaatctc gaaaatctag tctatttctc
      121 caacaatcga agaattatgc ccttttttct ccctttaaat aagtcataat acgaggcata
      181 catgcaacat ttacaataaa atatgttcct acatcgtatt atacgaatgc tcacacttat
      241 agttgtttag taaacccaga tgtattgcta acatacccat tatcactata tagtttatgt
      301 gcagatagtc tttcatttct attaccacta tttagtgtta ttgctttaca attcaaccgt
      361 ttagaaaatt cttcagaatc agctaataat ctctttccat aacctttttt cctaaattca
      421 gaatgtataa caaacgcaag gattctcata tactctgcat ttttttcgta aaacatcatt
      481 ttacacatac cacttaaacc aattatttta ttttctttta tcaaaagcag taggaagtaa
      541 tcatcatgat tcgttatttt ctttagtctc ttttttaaat cattctcatt tgttggataa
      601 cctagatcat catataattt acatagttga tttaattttt caaaatcact gttattaaac
      661 aatcttgtaa ttatgctcat ataacccacc ctaaaaatat tatgtaccta ttgttacata
      721 caatagatac gcaaaatcat ttctcactgc aaatcaggac gtttctcagc gtaaacaaat
      781 catttctatt cttttgaaaa atatctgcat atcttcacag taattttata acagattaat
      841 aaaattaaaa aaacataaca acaagtatta taataaatat tataatagct atcgtattaa
      901 atagtttaga atatgcaaac tgaaaaataa aaatcacaat taaaatacta agtaatgtaa
      961 atggtaatag catcatcaga tctttaacat tatttctttg ttcttgctcc caatcgctaa
     1021 tagccctaaa actacttttt ttatctatag gcgatgtaaa aataccatat attgaaggtg
     1081 ctatacctcc taaaatagca gttcccaaag ttgtcattac tgaaattact gcgaaagtat
     1141 catccgaaag caataaattc aaactaatgc attgtttatt acccatcgaa tttattgacc
     1201 aaatagctag agaaataaac aacccaaaat ttaaaataaa tgatatagta atagcaattg
     1261 tttacaaaac acgaaatttt tcatttttat ttatattatc catttttctc cctttttctt
     1321 aaatcatttt attatatatt acaataatca atctgaaatg ttgatgtaat ttgaaaaaaa
     1381 tatcatactt ttactcctga aaacctccct aaatcatcaa tatgaaaatc agtattagga
     1441 tattgcgttt acaactcttt taaatctcac tcattcttct catcgtctta accgatacta
     1501 tcaataaata ctggcaacac cataaaatat atacccaatt tataaaaacc taattgactc
     1561 ataatcttct aacggtatat catccacaat cacagtatga ttaggattat cgttagatac
     1621 atctttcact gccttatcta acccctcatc acctccgtcc cattcaccaa tattaatgaa
     1681 tataggtaca ttaccgttta tatcatgctt atctgtaaat aatttatgat atttacccaa
     1741 catatcacga gcttttaaac gatcactagg ttttattggt acctctatca gttcaacatg
     1801 ttcattatag actaattgta ctttgccact ttgtggattt tctttatatt ctccacgctt
     1861 gactacaact tctttcgttt ctgtttcatc accgactgcc gcattcgtta gcacatgtaa
     1921 taactctttt gcggttaata cattctcatc tataatctta tctttttgtt cttgtatata
     1981 ttgcttgatg tgcggctttt tcaataacct acacgctgtc acatgtgcgc tatttgcgtt
     2041 atatcctgct tttatggcac tttgtgttac attcagtgtt ctaatatact cattcacaaa
     2101 acgtgcttgc tttgcagtta actcactcat tttatcaccc ccacaatttt atctaatatg
     2161 gtttcatacc ataatattac agattgttct gaacaatcta aggcactact aatatcttga
     2221 taactaagtc cttgtataag ggagtcaaaa atataaaact ctttatcggt cgctaatctg
     2281 tcaacaatca tttctatgtg attctttata atatgatcat tgacattatc gtctgtcatc
     2341 aattcgtcag aatcttcatc acctattaaa aagaaatcat cagtatttat ttcatcatcg
     2401 ccccgttaac tagctttgaa gtctttagca cacttgcata taccggctgt cgtgctggcg
     2461 agatactagc attgaagtgg tctgatattg attttgaaaa caacacgatt agtattacta
     2521 aaacatatta caatccaaat aataacaaga agaaatatca gatacttccc cctaaaactg
     2581 aaagttctat cggtaagatt tccgttgacc caaatgtaat aaaggtgttg cgtgattata
     2641 agataaacgt tcaaaataat tggaaaaacg aattatataa tgataaccat tgtaatgaaa
     2701 aaactatcaa tgtggataca aaccattatg tcacgtacca gcatactcaa aaatatctct
     2761 acgcactcat tcagatacac acattgcgca ttgctcatag aatcaggtgt acacattaaa
     2821 gaaatacaag aacgattacg gcataaagat atcaatacca ctatgaatat ctatgctaaa
     2881 atcacaaatt catacaaaaa agacgcctcc cataagttta gtcaccaaat ggaagacgtc
     2941 tcgtaattaa aatatatttg cgtgcattct aatttatact tagaatgaat catactcgtg
     3001 cataatgtaa ttttctagtt agtcaaaact ataaacagtt ttacatcatt cctggcatgc
     3061 cacccatgtt aggttggtca ttattttttt ctggaattga tgctacaacc gcttcagtcg
     3121 ttaagaacat tgctgcaaca cttgcagcat gttgtaatgc tgagcgtgtt actttagttg
     3181 gatcaacgat accttcttct aacatattaa cccactcgtt tgtagcagcg ttaaaaccaa
     3241 cacccggctc tgcgtttttc aaacgttcta caataacaga accttctaat cctgcatttt
     3301 cagcaatttg acgaactggt gcagttaatg ctttaagtac aatatttaca cctgtttcaa
     3361 tgtcaccttc agcttcaatt tcacttactt tttggtaaac atttactaat gcagtaccac
     3421 cacctgcaac aataccttct tcaactgctg cacgtgtaga atttaatgca tcttcaatac
     3481 gtaatttacg ttctttaagc tctgtttcac ttgctgcacc tactttgata actgcaacac
     3541 cacctgctaa tttagctaag cgctcttgta atttttcacg atcaaagtca gattcagttt
     3601 cttcaatttg agatttcaat tggctaacac gtgcatcaat gctgttttcg tcaccgtcac
     3661 catcaacaac agtggtatta tctttagtta cttctacttt acttgcagta cctaacatat
     3721 caattgatgc atcttttaaa tctaagccta aatcatcagt aatcacttgc gcaccagtta
     3781 aaatagctaa atcttcaagc atcgcttttc tacgatcacc aaaaccaggt gcttttactg
     3841 caacagctgt aaatgtgcca cgcatacggt ttagcacgat atttgttaat gcatcgcctt
     3901 caacttcatc agctacaatt aagattggac gattagattg aaccacttgt tctaataaag
     3961 gtaagatatc ttggaaagac gagattttct tatctgttac taaaatgtat gggcgttcta
     4021 attcagcaac cattttatct gaatcagtaa ccatatacgg tgattgataa ccacgatcaa
     4081 attgcatacc ttcaaccact tctagttcag tgtttagtcc atttgattct tcaattgtaa
     4141 tgacaccatc gttacctact ttttccatag cttcagaaat ataacgtcca atttcttcat
     4201 ctgctgctga aatcgcacct acttgcgcaa tttcattttt attttcaact ttttgagaat
     4261 tttcatgtaa cgcttcaaca gcaactttaa ctgctttgtc gataccttgt cgtaaaccaa
     4321 ctgggttcgc accacttgta acatttttca agccttcttg aatcattgct tgagctaata
     4381 ctgttgcagt tgtcgtaccg tcaccagcaa tttcatttgt cttatttgcg acttcttgaa
     4441 ctagtttagc ccccatattt tcatatggat cttctaattc gatttcttta gcaatcgtca
     4501 caccatcatt cgtaattaaa ggtgctgtaa actctttatc taatacaaca ttacgtcctt
     4561 taggaccaat cgttacttta actgcatttg caagttggtc aacaccacgt aacattgctt
     4621 gacgtgcatc ttcagagaat ttcaattgtt taaccataaa tgataaacct ccatttgttt
     4681 agttgtgttt cattttcgtt ctatttaatt tatttatgaa ttaagttctg tattattcaa
     4741 taactgctaa aatatcttct tcatttaata ccagatatgt ttcattatct cgtttaactt
     4801 ctgtaccagc atattgttgg aacacgacac ggtccccttc tttcacttca ggagtcactc
     4861 ttgtaccatc atttaatagg cgtccagttc ctactgcaac gataacgcct tcgtttgatt
     4921 tttctttagc actatcagtt aaaacaatac cacttttagt tgtttgttct tgttcttttt
     4981 tctcaataat cacacgattt ccaattggtt ttagcatgat tgttcctcct taaaaaacct
     5041 aaagtttagc acttaacatt aaagagtgct aacatacatt tataataatc aaatttggtc
     5101 aaaatttcaa gtcataacct tttaattaag ttttgtaact tcaagttatt tacgataaaa
     5161 taaattataa acaaatattt ggaggaaaat tatgacaaga ttatgggcat cattgctaac
     5221 tgttattatt tatatattgt ctcaattttt accgcttctc attgtaaaaa aattaccatt
     5281 tgtacaatat agtggcatag aactgactaa agcagtcatt tacatacaac ttgttctatt
     5341 tttaatcgcc gccacgacga ttattttaat taatttaaaa attaaaaatc caacaaaatt
     5401 agaattagaa gttaaagaac ctaaaaaata tatcattcca tgggcattgc ttggatttgc
     5461 attggtaatg atttatcaaa tggtagtgag cattgtatta acgcaaattt atggtggaca
     5521 acaagtaagt cctaatacag aaaagctaat tattattgct cgaaaaatac ctatatttat
     5581 cttctttgta tctattattg gtcctttatt agaagaatat gtattcagaa aagtaatctt
     5641 tggagaatta tttaatgcga ttaaaggtaa tcgtatcgtg gcatttatta ttgctacaac
     5701 agtaagttca ttaatatttg cattagcaca taatgatttc aaatttattc cagtttattt
     5761 tggtatgggt gtcatttttt cattagcata tgtttggaca aaacggcttg ctgttccaat
     5821 tattatccat atgttacaaa acggatttgt cgttatattc caattactta atcctgaggc
     5881 tttgaaaaaa gccacggaac aagcgaattt tatatatcac atttttattc cataataaaa
     5941 aaaacgaatc acacacggat tatcgtcgct gtgattcgtt tttatttttc tttttatttc
     6001 gctttacaaa taagaatagc atgattgaaa ataccaccat catagaacat acaattgcta
     6061 gccaactttt actatatgca gttgacgcac ttttcttatt atcttcttgt tttttaacta
     6121 ctgtaaaatc gtctttttca tgcggttcaa atgagcgttg ttttgaatcc ggcttatctt
     6181 cgggcttttt caaatctggc attgatccaa tttcaccagt aattaatgcg agtacttgtt
     6241 catctaaatt acgatagtac ctttctgatt tcaatggatt gaaatattgg aatctaaagt
     6301 aattcgattg ctgttgtaat ccatttaaat atgcatttcc gctgaaattt tgttttcgaa
     6361 taatattata aatgtcttca tcagtaactt ctccataatc tttgcccaac ttattaattt
     6421 gatttaaaat atacggatta tacttgtaag ccccatttgc caaggctaaa aatcgttggg
     6481 atacaaaatc attaccagta ggattttgtg agtttccttg attcccattt ggttgccatt
     6541 gaccttgatt agatccatct gaagcatttg ggttccatgt ccccccattt ttcgagccac
     6601 cagaatgatt ggaatcccca ggttgatcgg gatctggtga cgggttagga tttggcttat
     6661 tagggtctgg ttttggattc ggatttggct ttggtttatc tggatctggt tttggatccg
     6721 gatttggctt attagggtct ggttttggat ctggatttgg tttcgggtta tctggatctg
     6781 gttttggatc cggatttggc ttattagggt ctggttttgg atctggattt ggtttcgggt
     6841 tatctggatc tggttttgga tccggatttg gctttggttt atctgggtct ggttttggat
     6901 ccggttttgg tttcgggtta tctggatctg gtttaggatt cggacttggg ttttggttat
     6961 ttgggtctgg ttttgaatct gtacttgggt tttggttgtt tgagtctggc ttcacattat
     7021 tcgaatcagg attagcatca cgactattcg aacctgttcc ttcattaccc gtttgatttg
     7081 attcgtgagg tgtcccagtc tcattattat tcatttcact atttactgat ggtgaagtta
     7141 tattatgatt caaattagta gttggttttt ctccatttaa gttatccttt gcttcaactg
     7201 tgtgcgttgc aagccctaaa ctactactac ttaaaattaa aatacttgtt gaaagtaaca
     7261 ttttcttaaa ccaatgatat gacatttcac gagacacctc cctttttcat gtaagtatta
     7321 atctaagtat aataaatatg cgttgaaaat gcatttatat ttggaa
//
LOCUS       DAKTIO010000132         7362 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.132, whole genome shotgun sequence.
ACCESSION   DAKTIO010000132 DAKTIO010000000
VERSION     DAKTIO010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7362
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.132"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            148..348
                     /locus_tag="O0293_002096"
     CDS             148..348
                     /locus_tag="O0293_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280969.1"
                     /translation="MTNPTRTKRCGGDVLYGIDNHFQLLLYISENHCQLETKTFFEYF
                     LRIVNKTHDYAILENKKICISH"
     gene            425..556
                     /locus_tag="O0293_002097"
     CDS             425..556
                     /locus_tag="O0293_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280970.1"
                     /translation="MLLPSSKSHIEMMCEMFKLRSFCENKDNNYSFYNSEKSVYDNN"
     gene            818..1477
                     /gene="arlR"
                     /locus_tag="O0293_002098"
     CDS             818..1477
                     /gene="arlR"
                     /locus_tag="O0293_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor ArlR"
                     /protein_id="HCY0280971.1"
                     /translation="MTQILIVEDEQNLARFLELELTHENYNVDTEYDGQDGLDKALSH
                     YYDLIILDLMLPSINGLEICRKIRQQQSTPIIIITAKSDTYDKVAGLDYGADDYIVKP
                     FDIEELLARIRAILRRQPQKDIIDVNGITIDKNAFKVTVNGAEIELTKTEYDLLYLLA
                     ENKNHVMQREQILNHVWGYNSEVETNVVDVYIRYLRNKLKPYDRDKMIETVRGVGYVI
                     R"
     gene            1474..2829
                     /gene="arlS"
                     /locus_tag="O0293_002099"
     CDS             1474..2829
                     /gene="arlS"
                     /locus_tag="O0293_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase ArlS"
                     /protein_id="HCY0280972.1"
                     /translation="MTKRKLRNNWIIVTTMITFVTIFLFCLIIIFFLKDTLHNSELDD
                     AERSSSDINNLFHSKPVKDISALDLNASLGNFQEIIIYDEHNNKLFETSNDNTVRVEP
                     GYEHRYFDRVIKKRYKGIEYLIIKEPITTQDFKGYSLLIHSLENYDNIVKSLYIIALA
                     FGVIATIITATISYVFSTQITKPLVSLSNKMIEIRRDGFQNKLQLNTNYEEIDNLANT
                     FNEMMSQIEESFNQQRQFVEDASHELRTPLQIIQGHLNLIQRWGKKDPAVLEESLNIS
                     IEEMNRIIKLVEELLELTKGDVNDISSEAQTVHINDEIRSRIHSLKQLHPDYQFDTDL
                     TSKNLEIKMKPHQFEQLFLIFIDNAIKYDVKNKKIKVKTRLKNKQKIIEITDHGIGIP
                     EEDQDFIFDRFYRVDKSRSRSQGGNGLGLSIAQKIIQLNGGSIKIKSEINKGTTFKII
                     F"
     gene            3113..5911
                     /locus_tag="O0293_002100"
     CDS             3113..5911
                     /locus_tag="O0293_002100"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCY0280973.1"
                     /translation="MTNERKEVSEAPVNFGANLGLMLDLYDDFLQDPSSVPEDLQVLF
                     STIKNDDSIVPALKSTSSQNSDGTIKRVMRLIDNIRQYGHLKADIYPVNPPKRKHVPK
                     LEIEDFDLDQQTLEGISAGIVSDHFADIYDNAYEAILRMEKRYKGPIAFEYTHINNNT
                     ERGWLKRRIETPYKVTLNNNEKRALFKQLAYVEGFEKYLHKNFVGAKRFSIEGVDALV
                     PMLQRTITIAAKEGIKNIQIGMAHRGRLNVLTHVLEKPYEMMISEFMHTDPMKFLPED
                     GSLQLTAGWTGDVKYHLGGIKTTDSYGTMQRIALANNPSHLEIVAPVVEGRTRAAQDD
                     TQRAGAPTTDHHKAMPIIIHGDAAYPGQGINFETMNLGNLKGYSTGGSLHIITNNRIG
                     FTTEPIDARSTTYSTDVAKGYDVPIFHVNADDVEATIEAIDIAMEFRKEFHKDVVIDL
                     VGYRRFGHNEMDEPSITNPVPYQNIRKHDSVEYVFGKKLVNEGVISEDEMHSFIEQVQ
                     KELRQAHDKINKADKMDNPDMEKPADLALPLQADEQSFTFDHLKEINDALLTYPDGFN
                     ILKKLNKVLEKRHEPFNKEDGLVDWAQAEQLAFATILQDGTPIRLTGQDSERGTFSHR
                     HAVLHDEQTGETYTPLHHVPDQKATFDIHNSPLSEAAVVGFEYGYNVENKKSFNIWEA
                     QYGDFANMSQMIFDNFLFSSRSKWGERSGLTLFLPHAYEGQGPEHSSARLERFLQLAA
                     ENNCTVVNLSSSSNYFHLLRAQAASLDSEQMRPLVVMSPKSLLRNKTVAKPIDEFTSG
                     GFEPILTESYQADKVTKVILATGKMFIDLKEALAKNPDESVLLVAIERLYPFPEEEIE
                     ALLAQLPNLEEVSWVQEEPKNQGAWLYVYPYVKVLVADKYDLSYHGRIQRAAPAEGDG
                     EIHKLVQNKIIENALKNN"
     gene            5925..7193
                     /gene="sucB"
                     /locus_tag="O0293_002101"
     CDS             5925..7193
                     /gene="sucB"
                     /locus_tag="O0293_002101"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCY0280974.1"
                     /translation="MPEVKVPELAESITEGTIAEWLKNVGDSVEKGEAILELETDKVN
                     VEVVSEEAGVLSEQLASEGDTVEVGQAIAIIGEGSGNASKENSNDNTPQQNEETNNKK
                     EETTNNSVDKAEVNQANDDNQQRINATPSARRYARENGVNLAEVSPKTNDVVRKEDID
                     KKQQAPASTQTTQQASAKEEKKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAMLT
                     TFNEVDMTNVMELRKRKKEQFMKDHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGDD
                     MITKQYYDIGVAVSTDDGLLVPFVRDCDKKNFAEIEAEIANLAVKAREKKLGLDDMVN
                     GSFTITNGGIFGSMMSTPIINGNQAAILGMHSIITRPIAIDQDTIENRPMMYIALSYD
                     HRIIDGKEAVGFLKTIKELIENPEDLLLES"
BASE COUNT         2774 a         1059 c         1331 g         2198 t
ORIGIN      
        1 tctgaaccat acgcatagcg tatggttttc tttttacaaa aaacaaaagc agtaaaccta
       61 aagtgtcgta agggtttact gcttttataa aacgttgtta taacgtatat tgtcttttac
      121 gggcatataa aaggggaata tttgaaaatg accaatccaa caagaacgaa acgttgtggg
      181 ggggatgttc tatatggtat tgataatcat tttcaactac tattatacat tagtgagaat
      241 cattgtcaat tagaaactaa aacttttttt gaatattttt taagaatagt aaataaaacg
      301 catgattacg ctattttaga aaataaaaaa atttgtattt ctcattagaa ttagaatatt
      361 taaaagtgat gaggtttaaa cattatattg tttacatact ccttttgaat tcatacatta
      421 tgaaatgtta cttccaagtt caaaatcgca cattgaaatg atgtgtgaaa tgtttaaact
      481 acggtcattt tgtgaaaata aagataataa ctattcattt tacaatagtg aaaagtcagt
      541 atatgacaac aattaatatt gcggtaaggc cttgtgttac agtattctat atttaagtac
      601 tgcaatcaga attaacagaa tgccattaac tgattattaa atatttgagt taataaataa
      661 ttaatgattg tagcttgaaa aatttaaaac atggttattg atttgtgata aaatttaaac
      721 gtaaacaaac taatttaaaa agcaactatt gtatagaaaa atacaaaatt taaaatatat
      781 taccttatta gaaaaagtcg taatatgagg tgtacaaatg acgcaaattt taatagtaga
      841 agatgaacaa aacttagcaa gatttcttga attggaactc acacatgaaa attacaatgt
      901 ggacacagag tatgatggac aagacggttt agataaagcg cttagccatt actatgattt
      961 aatcatatta gatttaatgt tgccgtcaat taatggctta gaaatttgtc gcaaaattag
     1021 acaacaacaa tctacaccta tcattataat tacagcgaaa agtgatacgt atgacaaagt
     1081 tgctgggctt gattacggtg cagacgatta tatagttaag ccgtttgata ttgaagaact
     1141 tttagcaaga attcgtgcaa ttttacgtcg tcagccacaa aaggatatta tcgatgtcaa
     1201 cggtattaca attgataaga acgcttttaa agtgacggta aatggcgcag aaattgaatt
     1261 aacaaaaaca gagtatgatt tactatatct tctagctgaa aataaaaacc atgttatgca
     1321 acgggaacaa attttaaatc atgtatgggg ttataatagt gaagtagaaa caaatgtcgt
     1381 agatgtttat ataagatatt tacgaaacaa gttaaaacca tacgatcgtg acaaaatgat
     1441 tgaaacagtt cgtggcgttg ggtatgtgat acgatgacaa aacgtaaatt gcgcaataac
     1501 tggattattg ttaccacgat gattacgttt gtcacgatat ttttgttttg tttaattatt
     1561 atttttttct tgaaagatac actgcataat agtgagcttg atgatgcaga acgaagctca
     1621 agcgatatta ataatttatt tcattctaag cctgttaaag atatatctgc attagacttg
     1681 aatgcatctt taggtaattt tcaagagata attatttatg atgagcataa taataaatta
     1741 tttgagacat cgaatgataa cacagtgaga gttgaaccag gttatgaaca ccgttatttt
     1801 gaccgcgtaa taaaaaaacg ctataaaggc attgaatatt taattattaa agaaccaatt
     1861 acaacgcaag atttcaaagg gtatagcttg ttaattcatt cactagaaaa ttatgataac
     1921 atcgtaaaat cattgtatat cattgcgctg gcatttggag tgattgcaac aattataact
     1981 gccacaatca gttatgtatt ttcaacacaa attactaaac cgcttgtcag tttatcaaat
     2041 aaaatgattg agattcgacg agatggtttt caaaataaat tgcaattaaa tacaaattat
     2101 gaagaaatag ataatttagc aaatacgttt aatgagatga tgagccaaat tgaagaatca
     2161 tttaatcaac aaagacaatt tgttgaagat gcgtcacatg aattacgaac accattacaa
     2221 attattcaag gtcatttaaa tttgattcag cgatggggaa aaaaagaccc agcagtatta
     2281 gaagaatcgt taaatatttc tattgaagaa atgaatcgta tcataaaatt agtcgaagaa
     2341 ttacttgaat tgactaaagg agatgtaaat gacatttctt ctgaagcaca gaccgtgcat
     2401 attaatgatg aaattcgctc gcgaatacac tcattaaaac aattgcatcc tgattatcaa
     2461 tttgatacgg atctgacatc taaaaatcta gaaattaaaa tgaaacctca tcaattcgaa
     2521 caattatttt taatctttat tgataatgca atcaaatatg atgtgaagaa taagaaaatt
     2581 aaagttaaga caaggttaaa aaataagcaa aaaataattg aaattacaga tcatggaatt
     2641 ggtattccag aggaagatca agatttcatt tttgatcgct tttatcgagt ggataaatct
     2701 cgttcaagaa gtcaaggcgg taatggactc ggattatcta ttgctcaaaa aatcattcaa
     2761 ttaaacggag gatcgattaa aattaaaagt gaaattaaca aaggaacaac gtttaaaatc
     2821 atattttaat catgactgag acgtcaatca aagtcatagg atcaattttt taagtacaca
     2881 ttagctgtga ctaatgtata agaacaacta taaaacaaat aaacagtggt tctttatcat
     2941 ttctgttgta ctcccaaaat ttacaataaa atacatctat aaacctagaa gaatcaacgc
     3001 ttttgttgat tcttcttttt agcagataaa taggtaaatc tactttaaca aataactaaa
     3061 tagtgatatt attacattgt aagcgtttca acatttttgt ggagggtgta aaatgactaa
     3121 cgaaagaaaa gaagtttcag aggctcctgt aaacttcggt gcgaatttag gtctaatgtt
     3181 agatctatat gatgactttt tacaagatcc atcatctgta ccagaagatt tacaagtctt
     3241 attcagcaca attaagaatg atgactcaat tgtaccagct ttaaaaagta caagtagtca
     3301 aaatagcgac ggcacaatta agcgtgtcat gcgtttaatt gataatattc gccaatacgg
     3361 gcatcttaaa gccgatattt atcctgtaaa tcctccaaaa aggaaacatg tacctaaatt
     3421 agagattgaa gactttgatt tagatcaaca gactttggaa ggtatatcag caggaattgt
     3481 ttcagatcac tttgccgaca tttatgataa tgcttatgaa gcaattttaa gaatggaaaa
     3541 acgttacaaa ggaccaattg catttgagta tacacatatt aataacaata ccgaacgtgg
     3601 ttggttaaaa agaagaattg aaacgccata taaagtaacg ttaaataata acgaaaaaag
     3661 ggcactattc aaacaattag cgtatgttga agggtttgaa aaatatcttc ataaaaactt
     3721 cgttggtgca aagcgttttt caattgaagg ggtagacgca cttgtaccga tgttacaacg
     3781 tactattacg attgctgcga aagaaggtat taaaaatata caaataggca tggctcaccg
     3841 tggacgttta aacgttttaa cgcatgtctt agaaaaaccg tacgaaatga tgatttcaga
     3901 atttatgcat acagatccaa tgaaattctt acctgaagat ggtagcttgc agttaactgc
     3961 tggatggact ggtgatgtga aatatcacct tggtggcatt aaaactactg attcatacgg
     4021 tacaatgcag cgtattgcac tggctaacaa tccaagtcac ttggaaattg ttgcacctgt
     4081 tgttgagggg cgtacgagag cagcacaaga tgatacacaa cgagctgggg ctccgacgac
     4141 tgatcatcat aaagcaatgc caattattat acatggcgat gctgcttatc ctggtcaagg
     4201 aattaacttc gaaacaatga acttaggaaa cttgaaaggc tattctacgg gtggttcatt
     4261 gcatattatt actaacaata gaattggatt tactacagaa ccaattgatg cacgttcaac
     4321 aacttattct acagatgtgg ccaaaggtta tgatgtgcca atattccatg tcaatgcaga
     4381 tgacgttgaa gctactattg aagcaattga tattgcaatg gaatttagaa aagagtttca
     4441 taaagacgtc gttattgatt tagtaggtta tcgtcgtttc ggacataacg aaatggatga
     4501 accatcaatt actaatccag ttccttatca gaatattcgc aaacatgact ctgttgaata
     4561 tgtgtttggt aaaaagcttg ttaatgaagg tgtcatttca gaagatgaaa tgcattcatt
     4621 tatagaacaa gtccaaaagg aactaagaca agctcatgat aaaattaata aagctgataa
     4681 aatggataat ccagatatgg aaaagcctgc agatcttgca ttaccgttac aagcagacga
     4741 acaatcattt acttttgatc acttgaaaga aataaatgat gcattgttaa catatccgga
     4801 tggctttaac attttgaaaa agttaaacaa agttcttgag aagcgtcatg agccgtttaa
     4861 taaagaagat ggtttagttg attgggcaca agcagaacaa cttgcatttg cgacaatttt
     4921 acaagatggt acaccgattc gcttaactgg tcaagatagt gaacgtggta cattcagtca
     4981 taggcatgcc gtgttacatg atgagcaaac aggtgaaaca tatacacctt tacatcatgt
     5041 tcctgatcaa aaagcgacat ttgatataca caattctccg ctttcagaag cagcagtagt
     5101 tggttttgaa tacggctata atgtggaaaa caaaaaaagc ttcaatattt gggaagcaca
     5161 atatggtgat tttgcaaata tgtcacaaat gatttttgac aacttcttat tcagttctcg
     5221 ctcaaaatgg ggagaacgtt caggattaac attattctta cctcatgcat atgagggtca
     5281 agggcctgaa cattcatcag caagattaga gcgattttta caattagctg ctgaaaataa
     5341 ttgcacagtt gtcaacttat ctagttcaag taattatttc cacttattgc gtgcacaagc
     5401 ggctagttta gattctgaac aaatgcgacc attggttgtt atgtcaccaa aaagcttact
     5461 gagaaataaa acagttgcaa aaccaattga tgaatttact tctggtggat ttgagccaat
     5521 tttgacagaa tcatatcaag cggataaggt tacaaaagtt attttggcaa ctggtaaaat
     5581 gttcattgat ttaaaagaag cattagctaa aaatccagac gaatcagtat tactcgttgc
     5641 gattgaaaga ttgtatccat tcccagagga agagattgaa gcattactag cacaattgcc
     5701 aaaccttgaa gaagtgtcat gggtacaaga agaacctaaa aatcaaggtg catggttata
     5761 tgtctatcca tatgttaaag tgctagttgc agataaatat gatttaagtt atcatggcag
     5821 aattcaaagg gctgctccag ctgaaggcga tggagaaatt cataaacttg ttcaaaataa
     5881 aattatagaa aatgcattaa aaaataacta gggggaaata agtcatgcca gaggttaaag
     5941 ttccagaatt agcagaatct attacagaag gtaccattgc agaatggttg aaaaacgtag
     6001 gggatagcgt agaaaaaggt gaagctattc ttgaattaga aactgataaa gttaatgtcg
     6061 aagttgtatc tgaagaagca ggtgtattat ctgaacaact tgcaagtgaa ggcgacactg
     6121 tagaagttgg acaagcaatt gctatcatcg gcgaaggtag tggcaatgct tctaaagaaa
     6181 atagtaacga caatactcca caacaaaatg aagaaacaaa taataaaaaa gaagaaacaa
     6241 caaataattc ggtagataaa gctgaagtaa atcaagcaaa tgatgacaat cagcaacgta
     6301 ttaatgctac gccttctgcg cgtcgatatg ctcgtgaaaa tggtgtgaat cttgctgaag
     6361 taagtccgaa aacaaatgat gtggttcgta aagaagatat tgataagaaa caacaggcac
     6421 cggcatcaac acaaacaaca caacaagcat ctgcaaaaga agagaaaaaa tacaatcaat
     6481 atcctacaaa accagtgatt cgtgaaaaaa tgtcacgtag aaagaaaaca gctgccaaaa
     6541 aattattaga ggtatctaat aatacagcta tgttaacaac atttaacgaa gttgacatga
     6601 caaatgttat ggaattgcgt aaacgtaaga aagaacaatt tatgaaagat catgatggta
     6661 ctaaattagg atttatgtca ttctttacta aagcttctgt agcagctttg aaaaagtatc
     6721 cagaagttaa tgcagaaatc gacggcgacg acatgattac gaaacaatat tatgatattg
     6781 gtgtagctgt ttctacagat gatggattat tagtaccatt tgtaagagat tgtgataaaa
     6841 agaattttgc agaaatcgaa gcagaaattg ctaatttagc agttaaagca cgagagaaaa
     6901 aacttggctt agatgatatg gttaatggtt catttacgat tacaaatggc ggtatttttg
     6961 gatcaatgat gagtacgcca attatcaatg gtaatcaagc tgcaatctta ggcatgcatt
     7021 caattattac aagaccaatt gcgattgatc aagatacaat cgaaaatcgt ccaatgatgt
     7081 atattgcatt aagctatgat catagaatta ttgacggtaa agaagcagtt ggattcttaa
     7141 aaacaattaa agaattaatt gaaaacccag aagacttatt attagaatct taatccacaa
     7201 cacaaaatag tttactattc ctaaaagcgg gattaaatca ataacaaaca gcagtaagat
     7261 tatttcctag tcgaaatatc ttactgctgt actttatttt tataatgatt cgtattaaaa
     7321 tgtgggttta attttgacta cttaaaaaat atatcatttc ta
//
LOCUS       DAKTIO010000133         7343 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.133, whole genome shotgun sequence.
ACCESSION   DAKTIO010000133 DAKTIO010000000
VERSION     DAKTIO010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7343
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.133"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            175..804
                     /locus_tag="O0293_002102"
     CDS             175..804
                     /locus_tag="O0293_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4887 domain-containing protein"
                     /protein_id="HCY0280975.1"
                     /translation="MSNQNYDYNKNEDGSKKKMSTTAKVVSIATVLLLLGGLVFAIFA
                     YVDHSNKAKERMLNEQKQEQKEKRQKENAEKERKKKQQEEKEQNELDSQANQYQQLPQ
                     QNQYQYVPPQQQAPTKQRPAKEENDDKASKDESKDKDDKASQDKSDDNQKKTDDNKQP
                     AQPKPQPQQPTPKPNNNQQNNQSNQQAKPQAPQQNSQSTTNKQNNANDK"
     gene            1564..2577
                     /locus_tag="O0293_002103"
     CDS             1564..2577
                     /locus_tag="O0293_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCY0280976.1"
                     /translation="MKDLLQAQQKLIPDLIDKMYKRFSILTTISKNQPVGRRSLSEHM
                     DMTERVLRSETDMLKKQDLIKVKPTGMEITAEGEQLISQLKGYFDIYADDNRLSEGIK
                     NKFQIKEVHVVPGDADNSQSVKTELGRQAGQLLEGILQEDAIVAVTGGSTMACVSEAI
                     HLLPYNVFFVPARGGLGENVVFQANTIAASMAQQAGGYYTTMYVPDNVSETTYNTLLL
                     EPSVINTLDKIKQANVILHGIGDALKMAHRRQSPEKVIEQLQHHQAVGEAFGYYFDTQ
                     GQIVHKVKTIGLQLEDLESKDFIFAVAGGKSKGEAIKAYLTIAPKNTVLITDEAAAKI
                     ILE"
     gene            2630..3640
                     /gene="gap"
                     /locus_tag="O0293_002104"
     CDS             2630..3640
                     /gene="gap"
                     /locus_tag="O0293_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCY0280977.1"
                     /translation="MAVKVAINGFGRIGRLAFRRIQEVEGLEVVAVNDLTDDDMLAHL
                     LKYDTMQGRFTGEVEVVDGGFRVNGKEVKSFSEPDASKLPWKDLNIDVVLECTGFYTD
                     KDKAQAHIEAGAKKVLISAPATGDLKTIVFNTNHQELDGSETVVSGASCTTNSLAPVA
                     KVLNDDFGLVEGLMTTIHAYTGDQNTQDAPHRKGDKRRARAAAENIIPNSTGAAKAIG
                     KVIPEIDGKLDGGAQRVPVATGSLTELTVVLEKQDVTVEQVNEAMKNASNESFGYTED
                     EIVSSDVVGMTYGSLFDATQTRVMSVGDRQLVKVAAWYDNEMSYTAQLVRTLAYLAEL
                     SK"
     gene            3779..4969
                     /locus_tag="O0293_002105"
     CDS             3779..4969
                     /locus_tag="O0293_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCY0280978.1"
                     /translation="MAKKIVSDLDLKGKTVLVRADFNVPLKDGEITNDNRIVQALPTI
                     QYIIEQGGKIVLFSHLGKVKEESDKAKLTLRPVAEDLSKKLDKEVVFVPETRGEKLEA
                     AIKDLKEGDVLLVENTRYEDLDGKKESKNDPELGKYWASLGDVFVNDAFGTAHREHAS
                     NVGISTHLETAAGFLMDKEIKFIGGVVNDPHKPVVAILGGAKVSDKINVIKNLVNIAD
                     KIIIGGGMAYTFLKAQGKEIGISLLEEDKIDFAKDLLEKHGDKIVLPVDTKVAKEFSN
                     DAKITVVPSDSIPADQEGMDIGPNTVKLFADELEGAHTVVWNGPMGVFEFSNFAQGTI
                     GVCKAIANLKDAITIIGGGDSAAAAISLGFENDFTHISTGGGASLEYLEGKELPGIKA
                     INNK"
     gene            5091..5852
                     /locus_tag="O0293_002106"
     CDS             5091..5852
                     /locus_tag="O0293_002106"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCY0280979.1"
                     /translation="MRTPIIAGNWKMNKTVQEAKDFVNALPTLPDSKEVESVICAPAI
                     QLDALTTAVKEGKAQGLEIGAQNTYFEDNGAFTGETSPVALADLGVKYVVIGHSERRE
                     LFHETDEEINKKAHAIFKHGMTPIICVGETDEERESGKANDVVGEQVKKAVAGLSEDQ
                     LKSVVIAYEPIWAIGTGKSSTSEDANEMCAFVRQTIADLSSKEVSEATRIQYGGSVKP
                     NNIKEYMAQTDIDGALVGGASLKVEDFVQLLEGAK"
     gene            5855..>7343
                     /locus_tag="O0293_002107"
     CDS             5855..>7343
                     /locus_tag="O0293_002107"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCY0280980.1"
                     /translation="MAKKPTALIILDGFANRESEHGNAVKLANKPNFDRYYNKYPTTQ
                     IEASGLDVGLPEGQMGNSEVGHMNIGAGRIVYQSLTRINKSIEDGDFFENDVLNNAIA
                     HVNSHDSALHIFGLLSDGGVHSHYKHLFALLELAKKQGVEKVYVHAFLDGRDVDQKSA
                     LKYIEETEAKFNELGIGQFASVSGRYYAMDRDKRWEREEKAYNAIRNFDAPTYATAKE
                     GVEASYNEGLTDEFVVPFIVENQNDGVNDGDAVIFYNFRPDRAAQLSEIFANRAFEGF
                     KVEQVKDLFYATFTKYNDNIDAAIVFEKVDLNNTIGEIAQNNNLTQLRIAETEKYPHV
                     TYFMSGGRNEEFKGERRRLIDSPKVATYDLKPEMSAYEVKDALLEELNKGDLDLIILN
                     FANPDMVGHSGMLEPTIKAIEAVDECLGEVVDKILDMDGYAIITADHGNSDQVLTDDD
                     QPMTTHTTNPVPVIVTKEGVTLRETGRLGDLAPTLLDLLNVEQPED"
BASE COUNT         2642 a         1160 c         1471 g         2070 t
ORIGIN      
        1 aaaatgaatc cgatttacga gtgagtaata gtgaaggcag ttttaagttg aagaaggcaa
       61 aaagagtaaa tgtttattaa tatttgtaga aactaggtaa gcaaattagt tgtgaaaatg
      121 ttaatggttg cgtgataatt tctatattta aattagtttg aagtgaggga gagtatgtcg
      181 aatcaaaatt acgactacaa taaaaatgaa gatggaagta agaagaaaat gagtacaaca
      241 gcgaaagtag ttagcattgc gacggtattg ctattactcg gaggattagt atttgcaatt
      301 tttgcatatg tagatcattc gaataaagct aaagaacgta tgttgaacga acaaaagcag
      361 gaacaaaaag aaaagcgtca aaaagaaaat gcagaaaaag agagaaagaa aaagcaacaa
      421 gaggaaaaag agcagaatga gctagattca caagcaaacc aatatcagca attgccacag
      481 cagaatcaat atcaatatgt gccacctcag caacaagcac ctacaaagca acgtcctgct
      541 aaagaagaga atgatgataa agcatcaaag gatgagtcga aagataagga tgacaaagca
      601 tctcaagata aatcagatga taatcagaag aaaactgatg ataataaaca accagctcag
      661 cctaaaccac agccgcaaca accaacacca aagccaaata ataatcaaca aaacaatcaa
      721 tcaaatcagc aagcaaaacc acaagcacca caacaaaata gccaatcaac aacaaataaa
      781 caaaataatg ctaatgataa gtagtattta gtcaaacaaa aatgaaccag tatgacagac
      841 aacacaatta attaggttgt ctcgaatatt ggttcttatt tttataattg ttaattaggg
      901 gagagatgat acttaaatag ttagttattt attttacgga tagtgaaatt tattttgagt
      961 gaggtgggac agaaatgata ttttcgcaaa atttatttcg tcgtcccacc ccaacttcca
     1021 ttgcctgtag aaattggaaa tccaatttct ctatgttggg gccccacccc aacttgcatt
     1081 gcctgtagaa attgggagga gccaatttca ctatgttggg gccccacccc aactcgcatt
     1141 gcctgtagaa tttcatttcg aaattctcta tgttggggcc cctgacttta attgaaaaaa
     1201 gcttgttaca agtgcatttt cgttcggtta actactacta atgtgacttt ttggattcta
     1261 gagcattgat ttatgtccta gtctcaaata ataagcaaat gattatcgag tcagtataag
     1321 ggtatacatt tgactacagc gaataaaata aaacgttttg ttgaataaca aatcgaaaat
     1381 atattgcaag cgctttatca aattatttag aaaatttaag ttttatgctt gcaatttttg
     1441 aaatagaata gtactattgc aagtgtaaag aggttaattt ttgtcccacg cgggacttaa
     1501 aaaggcaacc actggttgtg acatatcctt atttacattt ataaatataa ggaggaggta
     1561 gtagtgaaag acttattgca agcacagcaa aagcttatac cggatctcat agataaaatg
     1621 tataaacgtt tttctattct tactactatc tcaaaaaatc agcctgtcgg acgtcgaagt
     1681 ttaagcgaac atatggatat gactgaacgt gtactgcgtt ctgaaacaga tatgcttaag
     1741 aaacaagatt tgataaaagt taagcctacc ggaatggaaa ttacagctga aggtgagcaa
     1801 ctgatttcgc aattgaaagg ttactttgat atctatgcag atgataatcg tctgtcagaa
     1861 ggtattaaga ataaatttca aattaaggaa gttcatgttg ttcctggtga tgctgataat
     1921 agtcaatctg ttaaaacaga attaggtaga caagcaggtc aattacttga aggcatatta
     1981 caagaagacg cgatagttgc tgtaactggc ggatccacga tggcatgtgt tagtgaagca
     2041 attcatttat taccatataa tgtattcttc gtaccagcca gaggtggact aggcgaaaat
     2101 gttgtctttc aggcaaacac aattgcagcc agtatggcac aacaagctgg cggttattat
     2161 acgacgatgt atgtacctga taatgtcagt gaaacaacat ataatacatt gttgttagag
     2221 ccatcagtca taaacacttt agacaaaatt aaacaagcaa acgttatatt acacggcatt
     2281 ggtgatgcgc tgaagatggc gcatcgacgt caatcacctg aaaaggtcat tgaacaactt
     2341 caacatcatc aagctgtcgg agaggcattt ggttattatt ttgatacaca aggtcaaatt
     2401 gtccataagg ttaaaacaat tggacttcaa ttagaagacc ttgaatcaaa agactttatt
     2461 tttgcagttg caggaggcaa atcgaaaggt gaagcaatta aagcatactt gacgattgca
     2521 cccaagaata cagtgttaat cactgatgaa gccgcagcaa agataatact tgaataagag
     2581 ataaaaagtt taatactttt taaatatcat tttaaaggag gccattataa tggcagtaaa
     2641 agtagcaatt aatggttttg gtagaattgg tcgtttagca ttcagaagaa ttcaagaagt
     2701 agaaggtctt gaagttgtag cagtaaacga cttaacagat gacgacatgt tagcgcattt
     2761 attaaaatat gacactatgc aaggtcgttt cacaggtgaa gtagaggtag ttgatggtgg
     2821 tttccgcgta aatggtaaag aagttaaatc attcagtgaa ccagatgcaa gcaaattacc
     2881 ttggaaagac ttaaatatcg atgtagtatt agaatgtact ggtttctaca ctgataaaga
     2941 taaagcacaa gctcatattg aagcaggcgc taaaaaagta ttaatctcag caccagctac
     3001 tggtgactta aaaacaatcg tattcaacac taaccaccaa gagttagacg gttctgaaac
     3061 agttgtttca ggtgcttcat gtactacaaa ctcattagca ccagttgcta aagttttaaa
     3121 cgatgacttt ggtttagttg aaggtttaat gactacaatt cacgcttaca caggtgatca
     3181 aaatacacaa gacgcacctc acagaaaagg tgacaaacgt cgtgctcgtg cagcggcaga
     3241 aaacatcatc cctaactcaa caggtgctgc taaagctatc ggtaaagtta ttcctgaaat
     3301 cgatggtaaa ttagatggtg gtgcacaacg tgttcctgta gctacaggtt cattaactga
     3361 attaacagta gtattagaaa aacaagacgt aacagttgaa caagttaacg aagctatgaa
     3421 aaatgcttca aacgaatcat tcggttacac tgaagacgaa atcgtttctt cagacgttgt
     3481 aggtatgact tacggttcat tattcgacgc tacacaaact cgtgtaatgt cagttggcga
     3541 ccgtcaatta gttaaagttg cagcttggta tgataacgaa atgtcatata ctgcacaatt
     3601 agttcgtaca ttagcatact tagctgaact ttctaaataa ttttagtata gtttttattc
     3661 aaatacgcta gtgctcagaa ctatttagca ttaattaaag cttatgagta agcggggagc
     3721 acaaacgctt ctccgcttat ttttatataa aatttcctaa ttacaaggag gaaacaccat
     3781 ggctaaaaaa attgtttctg atttagatct taaaggtaaa acagtcctag tacgtgctga
     3841 ttttaacgta cctttaaaag acggtgaaat tactaatgac aaccgtatcg ttcaagcttt
     3901 acctacaatt caatacatca tcgaacaagg tggtaaaatc gtactatttt cacatttagg
     3961 taaagtgaaa gaagaaagtg ataaagcaaa attaacttta cgtccagttg ctgaagactt
     4021 atctaagaaa ttagataaag aagttgtttt cgtaccagaa acacgcggcg aaaaacttga
     4081 agctgctatt aaagacctta aagaaggcga cgtattatta gttgaaaata cacgttatga
     4141 agatttagac ggtaaaaaag aatctaaaaa tgatccagaa ttaggtaaat actgggcatc
     4201 tttaggtgat gtgtttgtaa atgatgcttt tggtactgcg catcgtgagc atgcatctaa
     4261 tgttggtatt tctacacatt tagaaactgc agctggattc ttaatggata aagaaattaa
     4321 gtttattggc ggcgtagtta acgatccaca taaaccagtt gttgctattt taggtggagc
     4381 aaaagtatct gacaaaatta atgtcatcaa aaacttagtt aacatagctg ataaaattat
     4441 catcggcgga ggtatggctt atactttctt aaaagcgcaa ggtaaagaaa ttggtatttc
     4501 attattagaa gaagataaaa tcgacttcgc aaaagattta ttagaaaaac atggtgataa
     4561 aattgtatta ccagtagaca ctaaagttgc taaagaattt tctaatgatg ccaaaatcac
     4621 tgtagtacca tctgattcaa ttccagcaga ccaagaaggt atggatattg gaccaaacac
     4681 tgtaaaatta tttgcagatg aattagaagg tgcgcacact gttgtatgga atggacctat
     4741 gggtgtattc gagttcagta actttgcaca aggtacaatt ggtgtatgta aagcaattgc
     4801 aaaccttaaa gatgcaatta cgattatcgg tggcggtgat tcagctgcag cagcaatctc
     4861 tttaggtttt gaaaatgact tcactcatat ttcaactggt ggcggcgcgt cattagagta
     4921 cctagaaggt aaagaattgc ctggtatcaa agcaatcaat aataaataat aaagtgatag
     4981 tttaaagtga tgtggcatgt ttgtttaaca ttgttacggg aaaacagtca caagatgaca
     5041 tcgtgtttca tcacttttca aaaatattta caaaacaagg agtgtcttta atgagaacac
     5101 caattatagc tggtaactgg aaaatgaaca aaacagtaca agaagcaaaa gacttcgtca
     5161 atgcattacc aacattacca gattcaaaag aagtagaatc agtaatttgt gcaccagcaa
     5221 ttcaattaga tgcattaact actgcagtta aagaaggaaa agcacaaggt ttagaaatcg
     5281 gtgctcaaaa tacgtatttc gaagataatg gtgcgttcac aggtgaaacg tctccagttg
     5341 cattagcaga tttaggcgtt aaatacgttg ttatcggtca ttctgaacgt cgtgaattat
     5401 tccacgaaac agatgaagaa attaacaaaa aagcgcacgc tattttcaaa catggaatga
     5461 ctccaattat atgtgttggt gaaacagacg aagagcgtga aagtggtaaa gctaacgatg
     5521 ttgtaggtga gcaagttaag aaagctgttg caggtttatc tgaagatcaa cttaaatcag
     5581 ttgtaattgc ttatgaacca atctgggcaa tcggaactgg taaatcatca acatctgaag
     5641 atgcaaatga aatgtgtgca tttgtacgtc aaactattgc tgacttatca agcaaagaag
     5701 tatcagaagc aactcgtatt caatatggtg gtagtgttaa acctaacaac attaaagaat
     5761 acatggcaca aactgatatt gatggggcat tagtaggtgg cgcatcactt aaagttgaag
     5821 atttcgtaca attgttagaa ggtgcaaaat aatcatggct aagaaaccaa ctgcgttaat
     5881 tattttagat ggttttgcga accgcgaaag cgaacatggt aatgcggtaa aattagcaaa
     5941 caagcctaat tttgatcgtt attacaacaa atatccaacg actcaaatcg aagcgagtgg
     6001 cttagatgtt ggactacctg aaggacaaat gggtaactca gaagttggtc atatgaatat
     6061 cggtgcagga cgtatcgttt atcaaagttt aactcgaatc aataaatcaa ttgaagacgg
     6121 tgatttcttt gaaaatgatg ttttaaataa tgcaattgca cacgtgaatt cacatgattc
     6181 agcgttacac atctttggtt tattgtctga cggtggtgta cacagtcatt acaaacattt
     6241 atttgctttg ttagaacttg ctaaaaaaca aggtgttgaa aaagtttacg tacacgcatt
     6301 tttagatggc cgtgacgtag atcaaaaatc cgctttgaaa tacatcgaag agactgaagc
     6361 taaattcaat gaattaggca ttggtcaatt tgcatctgtg tctggtcgtt attatgcaat
     6421 ggatcgtgac aaacgttggg aacgtgaaga aaaagcttac aatgctattc gtaattttga
     6481 tgccccaact tatgcaactg ccaaagaagg tgtagaagca agctataatg agggcttaac
     6541 tgacgaattc gtagtaccat tcatcgttga gaatcaaaat gacggtgtta atgatggaga
     6601 tgcagtgatc ttctataatt tccgacctga tagagcagcg caattatcgg aaatttttgc
     6661 gaacagagca ttcgaaggct ttaaagttga acaagttaaa gacttattct atgcaacatt
     6721 cactaagtat aatgacaata tcgatgcggc tatcgtcttc gaaaaagttg atttaaataa
     6781 tacaattggt gaaattgcac aaaataacaa tttaactcaa ttacgtattg cagaaactga
     6841 aaaataccct cacgttactt actttatgag tggtggacgt aacgaggaat ttaaaggtga
     6901 acgccgtcgt ttaattgatt cacctaaagt tgcaacgtat gacttgaaac cagaaatgag
     6961 tgcttatgaa gttaaagatg cattattaga agagttaaat aaaggtgact tggacttaat
     7021 tattttaaac tttgctaacc ctgatatggt tggacatagt ggtatgcttg agccgacaat
     7081 caaagcaatc gaagcggttg atgaatgttt aggagaagtg gttgataaga ttttagacat
     7141 ggacggttat gcaattatta ctgctgacca tggtaactct gatcaagtat tgacggatga
     7201 tgatcaacca atgactacgc atacaacgaa cccagtacca gtgattgtaa caaaagaagg
     7261 cgttacactt agagaaactg gtcgcttagg tgacttagca cctacattat tagatttatt
     7321 aaatgtagaa caacctgaag ata
//
LOCUS       DAKTIO010000134         7270 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.134, whole genome shotgun sequence.
ACCESSION   DAKTIO010000134 DAKTIO010000000
VERSION     DAKTIO010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7270
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.134"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..459)
                     /locus_tag="O0293_002108"
     CDS             complement(<1..459)
                     /locus_tag="O0293_002108"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HCY0280981.1"
                     /translation="MKKLNIALLGLGTVGSGVVKIIEENRQQIQDTLNKDIVIKHILV
                     RDKSKKRPLNISQYHLTEDVNEILNDDSLDIIVEVMGGIEPTVDWLRTALKNKKHVIT
                     ANKDLLAVHLKLLEDLAEENGVALKFEASVAGGIPIVNAINNGLNANNISK"
     gene            650..2032
                     /locus_tag="O0293_002109"
     CDS             650..2032
                     /locus_tag="O0293_002109"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCY0280982.1"
                     /translation="MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDN
                     DIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYIN
                     QLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQIL
                     DSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENF
                     TDVSGIYKANPNIIKDPELIEEITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKN
                     TNRPQDKGTFIVHDREINAKNVISGISCDKDFTVINIKKYLMNRQVGFTRKILGVLED
                     NNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGE
                     GMNKVIGTANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHG
                     VALKNLCDKN"
     gene            complement(2086..2661)
                     /locus_tag="O0293_002110"
     CDS             complement(2086..2661)
                     /locus_tag="O0293_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280983.1"
                     /translation="MNAKEQLVDNLMKTSSQLFKFHGEVAMQLFLNDELKLPSIVEIC
                     VERKRLSDIVKVIPQSYALLYIDKQDQAIAKEDLSLSKIAKVYVQYDDTTIMSIFVYD
                     VVNDEWIFRLDPNIRIPKSNIYFHSLNWDVDYIKPEIVLMYDLMQHHQYHHYSNYKRV
                     IDALSYYQFFILKFVVGEQRIKDAIQRTINN"
     gene            2992..3846
                     /locus_tag="O0293_002111"
     CDS             2992..3846
                     /locus_tag="O0293_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0280984.1"
                     /translation="MSFYVVLIIIIVALIGILVLNQRYSNSKIDTEVYARKQLIKKNK
                     ALSAENAELRSQMLSSNNDVGHHAYKNAKRELRKILDSYLENGKLKYYDIIVTSNLAT
                     KHPFFEYARSFDFIIVSDIGLINVDVKSWGEKTFYHFDVPDEHDTEISNSNIEKVVGH
                     YISQQYHDQFNSSRKSIYTFTETVQPNRVIYDFYDYDPYQLAANNAKALKDHIEQNFN
                     FKVQSTGVIYFSDGTVNIIQGSEERDKYVDTVSTKSSLRRIISEAIELSKHPLNKEQV
                     DQITAIFK"
     gene            complement(3989..4522)
                     /locus_tag="O0293_002112"
     CDS             complement(3989..4522)
                     /locus_tag="O0293_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCY0280985.1"
                     /translation="MKSNKSLAMIVVAIIIVGVLAFQFMNHTGPFKKGTNHETVQDLN
                     GKDKVHVQRVVDGDTFIANQNGKEIKVRLIGVDTPETVKPNTPVQPFGKEASNYSKKT
                     LTNQDVYLEYDKEKQDRYGRTLAYVWISKDRMYNKELVEKGLAREKYFSPNGKYRNVF
                     IEAQNKAKQQKLNIWSK"
     gene            4661..4849
                     /locus_tag="O0293_002113"
     CDS             4661..4849
                     /locus_tag="O0293_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HCY0280986.1"
                     /translation="MNISNENTVTLIAVIIAIIIGIFLQIFFKLPLIVTAVLSILLGI
                     FVGFIVYLIVSFYNKRKN"
     gene            complement(4962..5564)
                     /locus_tag="O0293_002114"
     CDS             complement(4962..5564)
                     /locus_tag="O0293_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0280987.1"
                     /translation="MTSLIIAEDQNMLRQAMVQLIKLHGDFEILADTDNGLDAMKLIE
                     EYNPNVVFLDIEMPGMTGLEVLAEIRKKHLNIKVIIVTTFKRPGYFEKAVVNDVDAYV
                     LKERSIEELVETINKVNNGEKEYSATLMTSFFVDKNPLTPKEQIVLREIGNGLSSKEI
                     SEKLFLTDGTVRNYTSVIIDKLFADNRFDAWKKANEKGWI"
     gene            complement(5561..6652)
                     /locus_tag="O0293_002115"
     CDS             complement(5561..6652)
                     /locus_tag="O0293_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCY0280988.1"
                     /translation="MKFLKDTSIAEISSIVYLIFPIAGIFFNEVYGPKWLYIISVIVF
                     SLSYLILVIVNNRLNTLMFYILLIIHYFIICYFVFSVHPMLSLFFFYSAFAVPFTFKN
                     NVKKTATNLFILTMIICTIITYLLYNNYFVAMMVYYVVISLIMLDNFKKMKNREYQKE
                     IAEKNRHINTLIAEQERHRIGQDLHDTLGHVFASLSLKSELAYKLIDADVEKVKAELL
                     AINKLSRESLNKVREIIDDVKLPSFIEEIDSIRKVLKDADIDFTFENKELAQVLSPTK
                     QSMLVMITREAINNVIKHANASKVHGKLKTVNNHKLLLMIEDDGKGIDSDCEVKSISQ
                     RVQHLNGTLAVDSTNGTKIIIEISTGGIA"
     gene            complement(6656..>7270)
                     /locus_tag="O0293_002116"
     CDS             complement(6656..>7270)
                     /locus_tag="O0293_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0280989.1"
                     /translation="LPEDVKPKFYKEYMYSMTVYSLLSFSLLTFPLDIINEKQNEWRQ
                     RLMVTPFTFTSYYISKVVKTMLQFAIAILVIFMVGHFYKGVAMSAVQWLESGIFLWLG
                     ASLLITFGILFSLLNDIQKTSALANIVTIGLAVLGGLWFPINTFPNWLQHVAHVLPSY
                     HLRKLGVDIASNHHINLISFAIILLYALGSIIAVYCISHFKRAE"
BASE COUNT         2555 a         1148 c          966 g         2601 t
ORIGIN      
        1 ttttgaaata ttattcgcat tcaaaccatt atttatggca tttacgatcg gaataccacc
       61 tgctacactc gcttcaaact ttaaagctac accattttct tctgctaaat cttctaaaag
      121 tttaagatgt actgctaata aatctttatt tgcggtaata acatgttttt tatttttaag
      181 tgctgttctt aaccaatcta cagttggttc aattcctccc atgacttcaa cgataatatc
      241 taatgaatca tcatttaaaa tttcattaac atcttcagtt aaatgatatt ggctaatatt
      301 tagcggtctc tttttagatt tatctcgaac aagaatatgc tttatgacaa tatctttatt
      361 taatgtatct tgaatttgct gtcggttctc ttcgatgatt ttaacaacac cagatccgac
      421 agtacctaat cctaataatg ctatatttaa ttttttcatt ttatgtacct ccgtttgtgc
      481 ttttgagaaa aacaaatgta ttattgaaaa acactagatg atagtatagt atagaattat
      541 tctttatttt tgtaaagttc gtaatattca aaatataaat acataatatc attcttactt
      601 acaaaactga atacaatttt ctgatttttt agaaaggttg gcatgcacta tgaaagtttc
      661 caaattcggt ggtagctccg tctccaatgc tactcaaatt aaaaaggttt taaatattgt
      721 taattctgat ccagagcgaa agattgttat cgtttctgct ccaggtaaaa gacatgataa
      781 tgatattaaa acaactgatt tgttaatcag attatatgaa aaggtcatta atcatcttga
      841 ttatcatgat aaaaaaagag aaattattca gcgttatgat gatattgtaa aagaattgca
      901 aatggatgaa agtattttac ggacgataga tgtgactttg gaacattata ttaatcaatt
      961 aaaaaatgaa ccaaagagac tattagatgc attactttct tgtggtgaag attttaatgc
     1021 gcaattgata gctttatata ataatagtca aggtatacca acaaaatata tatcaccgaa
     1081 ggaagcgggt atttttgtta ctgacgtccc acaagaagca caaatattag atagtgctta
     1141 ctctgaaatt aaaaaattaa gagattatga tgaaaaacta attatcccag gattttttgg
     1201 cgtatctcat gaaggatata tagttacatt tccacgtggc ggatcagata taactggcgc
     1261 catcatatca agtggtgtta gagccacaat ttatgaaaat ttcaccgatg tgtctggtat
     1321 ttataaagct aatccaaata tcatcaaaga tcctgaatta atcgaagaaa tcacttatcg
     1381 agaaatgcgt gaattatctt atgctggttt tagtgttttc catgatgaag ccttacaacc
     1441 actttataaa gatcgcatcc ctgtggtgat taaaaatacg aaccgccctc aagataaggg
     1501 tacatttata gtgcatgata gagaaataaa tgcgaaaaat gtgattagcg gtataagttg
     1561 tgataaagat tttaccgtta ttaatatcaa aaagtattta atgaatagac aagtcggctt
     1621 tactagaaag atattaggtg tcttagagga taataatatt tcatttgatc atatgccttc
     1681 aggtattgat actatcagta tcgttatgcg ttcaaaacaa attcaaaata aagaaacaaa
     1741 agtattaaat gaaattcgtc aaaaatgtga tgttgatgag ctaagtattg agcatgattt
     1801 agcaatatta atgattgtcg gcgaaggtat gaataaagtc atcggtactg caaataaaat
     1861 tacacatgcc ttagctgaat caaaaattaa cttaaaaatg attaaccaag gtgcttcgga
     1921 aatctcgatg atgtttggaa ttcatgtaga tgatgctgaa aaagctgtat tatctacata
     1981 tgagttttgc tatcacgggg ttgcattaaa aaacttgtgt gataaaaact aatataaaag
     2041 caattcgtaa cacatgtatt caactgcgtt atgaattgct ttttcttaat tatttattgt
     2101 tctctggatt gcatccttaa tacgttgctc acctactaca aattttaaaa taaaaaattg
     2161 atagtagctt aatgcatcta tgactcgttt ataattggaa taatgatgat actgatggtg
     2221 ttgcattaga tcatacatta gaacgatctc cggtttaata taatccacat cccaatttaa
     2281 actatggaag tatatgttac tcttaggtat acgtatattc ggatccaatc taaaaatcca
     2341 ttcatcgttt actacatcat aaacgaaaat actcattatt gttgtatcat catattgcac
     2401 ataaactttt gcaatttttg aaagtgataa atcttcttta gctattgctt gatcttgctt
     2461 atctatgtat agtaacgcat atgattgcgg aataactttc acaatatcac ttaaacgctt
     2521 acgttccacg catatttcaa caatagaagg taattttaat tcatcattta agaaaagctg
     2581 catggcaact tcaccgtgaa atttaaataa ttgcgatgat gttttcatta aattgtccac
     2641 taattgttct ttagcattca tttcattcac tctcaaatac acctcctaag caagcgcttt
     2701 cacaaatatc attatacatt ttaaaattaa tgaattcaat cttcaaaata ctatgagtga
     2761 cctaattctg tcatttaaat gaccgttata acataaattc gacataaact tgaattcacg
     2821 aatctttcac gtcatattca ttgaacatca atatctgtca gtttataatt tgattataac
     2881 aatgatgaac taaaatcaac taataaaaat gtgggaaatt gaataacaat taatctttat
     2941 taattattat tgtatacaaa gttaaagaag tcaaaaagga ttggtgaaaa aatgtctttt
     3001 tatgttgtac ttatcattat cattgtagca ttaattggta tattagtttt aaatcaaagg
     3061 tatagtaata gtaaaataga taccgaagtt tatgcaagaa agcaacttat taaaaagaac
     3121 aaagcattaa gtgcagaaaa tgctgaatta agaagtcaaa tgctgagttc taataatgat
     3181 gttggccacc atgcatacaa aaatgctaaa agagaattac gcaagatttt agatagctat
     3241 ctcgaaaatg gtaaattaaa atattacgat attatcgtta caagcaattt agctactaaa
     3301 catccgtttt tcgagtatgc acgttcattt gattttatca ttgtttcaga tatcggttta
     3361 ataaacgttg atgtaaaaag ctggggtgaa aaaacatttt atcactttga tgtacctgat
     3421 gagcatgata cagaaataag caatagtaat attgaaaaag ttgtcggtca ttacattagc
     3481 cagcaatatc atgatcagtt taattcatca agaaagtcta tctatacttt tacagaaaca
     3541 gttcaaccaa atcgtgttat ttatgatttt tatgattatg atccgtatca attagcagca
     3601 aataatgcga aagcattaaa ggatcatatt gaacaaaact ttaactttaa agtccaaagt
     3661 accggtgtta tttactttag tgatggtaca gttaatatca ttcaaggttc tgaagaaaga
     3721 gataaatatg ttgatacagt ttcaacaaaa tcttcgttac gacgtattat tagcgaagcc
     3781 atagagctat ctaaacaccc tttaaataaa gaacaggttg atcaaatcac agctatattc
     3841 aagtaaaatc aaaggcataa taatccatta cgctcgatac gcactatggt tattatgcct
     3901 ttaaattatt tctatatata aatttatggt aataataaat cgatgcttca agtatatttt
     3961 actttaatat atataatcac ctatttaatt atttactcca aatatttaat ttctgttgtt
     4021 tagctttatt ttgtgcttct ataaatacat ttctatattt gccatttggt gaaaaatact
     4081 tctctctagc aagtcccttt tccactaatt ccttattgta catacgatct ttacttatcc
     4141 atacatacgc caatgttcta ccatagcgat cttgtttttc tttatcatat tctaaataaa
     4201 catcttgatt tgttaatgtc ttcttactat aatttgatgc ttctttgcca aatggttgta
     4261 caggcgtatt cggtttcacc gtttctggcg tatcaacccc tataagccta actttaattt
     4321 ctttaccatt ttgatttgca ataaatgtat caccatccac aactctttga acatgtactt
     4381 tatctttacc atttaaatct tgtacagttt catgattcgt cccctttttg aaaggacccg
     4441 tatgattcat aaattgaaat gctaatacac ctacaataat gatggctacc acaatcatag
     4501 caagcgattt atttgacttc atattgccac ctacttctct tttttcattc cgattaatca
     4561 tataaaagtt aagaaatatc gtcaatagcc atatctacat atttcaatat tttggaaagc
     4621 tatagcatga tataatgtta ttgttaagga gtgaaaataa atgaacattt caaatgaaaa
     4681 tacagtcaca ttaattgcag taattatcgc aattatcatt ggtattttcc tacaaatatt
     4741 tttcaaatta ccattaatag tgacggcagt actatctatt ttattgggta tctttgtagg
     4801 ctttatcgtt tacttgattg tttcgtttta taataagcga aaaaattagt taacaaatct
     4861 aaattgcgag taccaaaata aaagtattga gtaagatttt gattattgac tcactttcca
     4921 agtctcttaa atcaaaacac tcaatacttt tttgtttgta tttagatcca gcctttttca
     4981 tttgcctttt tccaagcatc aaaacgatta tctgcaaata atttatcaat tataacagat
     5041 gtataatttc taactgttcc atctgtcaaa aataattttt cacttatttc tttactactt
     5101 aaaccattgc caatttccct taatacaatt tgttctttgg gcgttaatgg gtttttatct
     5161 acaaaaaatg aagtcatcaa tgtggcgcta tattctttct ctccgttatt tactttatta
     5221 atggtttcca ccaattcttc tatagaacgt tcttttaaaa catatgcatc cacatcattc
     5281 acaactgctt tttcaaagta tcccggtctt ttaaaagttg ttacaataat cactttaata
     5341 ttcaaatgct tttttctaat ttccgctaaa acttcaagtc cagtcatgcc tggcatttct
     5401 atatctaaaa aaacaacgtt aggattatat tcttcaataa gtttcattgc atcgagacca
     5461 ttatcagtat ctgctaaaat ttcaaaatca ccatgtagtt taattaattg aaccattgcc
     5521 tgtcgtaaca tattttgatc ttctgcaata attaaagatg tcatgctatt cctcctgttg
     5581 agatttcaat gattatttta gttccatttg ttgagtcgac tgctaaagtt ccatttaaat
     5641 gttgtacacg ctgtgaaata cttttcacct cacaatcact atcgatacct ttgccatcat
     5701 cttcaatcat aagcagtaat ttatgattgt ttacagtttt taatttacca tgaacttttg
     5761 aagcatttgc atgtttaata acattattta tcgcttcacg cgtaatcata actaacatag
     5821 attgtttagt aggacttaat acttgcgcta attctttatt ttcaaatgta aaatcaatat
     5881 cagcatcttt taaaacttta cgtatactat caatctcttc aataaatgat ggtaatttta
     5941 catcatcaat aatttctcga actttgttca atgattcacg agataattta ttaattgcta
     6001 ataattcagc ttttactttt tctacatcag catctattag tttataagct aattctgatt
     6061 ttaatgataa acttgcaaac acatgcccta acgtatcatg taagtcttga ccaattctat
     6121 gtcgctcttg ttcagcaatt aatgtattaa tatgtctatt tttttctgct atttcttttt
     6181 gatattcacg gtttttcatt tttttaaaat tatctagcat tattaacgat ataacgacat
     6241 aataaaccat cattgcaaca aaatagttgt tatacaataa gtacgttatt attgtacata
     6301 taatcattgt tagtatgaaa agattagttg ccgttttttt aacattattt ttaaaagtaa
     6361 atggaacggc aaaagcacta taaaagaaaa acaaacttag cattggatgt acactgaaaa
     6421 caaaataaca aataataaaa taatgaataa tcaacaaaat gtaaaacatt aatgtattaa
     6481 gtctattatt tactataact aatataagat acgacaacga aaagacaatg actgatataa
     6541 tatacaacca tttgggacca tatacttcat taaaaaatat accggcaata ggaaaaatca
     6601 gataaactat agacgatatt tcagcaattg aagtatcttt taaaaatttc atattttatt
     6661 ccgccctttt aaaatgacta atacaatata ctgctattat actccctaaa gcatacaaga
     6721 gtattatagc aaatgatatt aaattgatat gatgatttga agcaatatct acacctagtt
     6781 tacgcaaatg atagctcggt aaaacatgag caacatgttg aagccaattt ggaaatgtgt
     6841 ttatcggaaa ccacaatcca cctaatactg ctaaaccaat tgttacgata ttagctaaag
     6901 cacttgtttt ttgaatatca tttaacaaag aaaataatat gccaaaagtt attaatagag
     6961 acgcacctaa ccataaaaat attcctgact ctaaccattg aactgcactc attgcaacac
     7021 ctttataaaa atgtccaacc ataaaaataa ctaatatcgc tattgcaaat tgcagcatag
     7081 ttttcactac ttttgaaata taataactag taaaagtaaa tggtgttacc attaatcttt
     7141 ggcgccattc attttgtttt tcattaataa tatctaatgg aaaagttagt aaactaaaac
     7201 ttaacaaact ataaaccgtc atactataca tatactcttt ataaaattta ggtttaacat
     7261 cttccggcaa
//
LOCUS       DAKTIO010000135         7267 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.135, whole genome shotgun sequence.
ACCESSION   DAKTIO010000135 DAKTIO010000000
VERSION     DAKTIO010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7267
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.135"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            260..634
                     /locus_tag="O0293_002117"
     CDS             260..634
                     /locus_tag="O0293_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HCY0280990.1"
                     /translation="MKRILVVFLMLAIILAGCSNKGEKYQKDIDKVYKEQNQMNKIAS
                     KVQNTIKTDIKQEDSNTHVYKDGKVIVIGIQLYKDREKMYYFAYEIKDGKAEINREID
                     PIKYMKDHKADYEDENVEVEKD"
     gene            787..1185
                     /locus_tag="O0293_002118"
     CDS             787..1185
                     /locus_tag="O0293_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCY0280991.1"
                     /translation="MEKSIKIMTIIGIVVQGLATVFSLLLMVLAASGVMTTDVSTTVN
                     GEVDPVDAETAAAIFTVLFLSLFIFGIISIILGAIGMFKASKNKKMSGILLIIGAVIS
                     GNIITFALWLISGIKLLTNNKPKDEISDLS"
     gene            complement(1401..2225)
                     /locus_tag="O0293_002119"
     CDS             complement(1401..2225)
                     /locus_tag="O0293_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCY0280992.1"
                     /translation="MKEKHIKWDKIFYGDDWVNNVVETIRTKDILQSVYLKRYLLRAM
                     MAGFIIGIITVFVLSVKATHEPDLPPGIVNMASAITFSFALVLILFTNSELLTSNFMY
                     FTVGLYYKVIKPTRVLKIFLLCFAGNILGAAILFSFMRFSNVMTPDMLNQLSAVIEHK
                     TLSTGFVSILMKAIFANFFINISLVIAMQIDDVLAKMFVMMFGVTIFAFMGYEHVVYN
                     SCLFMGGLIYQVDTLHFIPAISNIAAAFIGNYIGGGLIIGLFYAYLNDHHQFYKNN"
     gene            complement(2462..3769)
                     /gene="brnQ"
                     /locus_tag="O0293_002120"
     CDS             complement(2462..3769)
                     /gene="brnQ"
                     /locus_tag="O0293_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCY0280993.1"
                     /translation="MKKQVIISGLMLFSLFFGAGNLIFPPMLGHTAGQNMWIGMLGFA
                     LTGILLPFITVIVVAFYDEGVESVGNRIHPWFGFIFAVVIYMSIGAFYGIPRAANVAY
                     EIGTRHILPVHNQWTLIIFAAIFFAIVYWISLNPSKIVDNLGKLLTPLLLLMVALLSI
                     AVIFNPESALSAPKDKYITHPFISGSLEGYFTMDLVAALAFSVVIVNGYKFKGLTDRM
                     KILKYVCFSGLIAAILLGMIYFALAYVGASTAPGNFKDGTDILTYNSLRLFGSFGNLV
                     FGMTVILACLTTCIGLVNACATFTKKHVPKFSYKIFALIFSIIGFLFTTLGLEMILKI
                     AVPLLTLIYPVSIALVLISFANMFSTFRFSWAYRLATVITLIISILQILNSFNLLHGV
                     ILKSFMMLPLADIDLAWLVPFMLFAIIGFIIDVFIRRPKQATT"
     gene            4353..5243
                     /locus_tag="O0293_002121"
     CDS             4353..5243
                     /locus_tag="O0293_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HCY0280994.1"
                     /translation="MNKISKYIAIASLSVAVTVSAPQTTNSTAFAKSSAEVQQTQQAS
                     IPASQKANLGNQNIMAVAWYQNSAEAKALYLQGYNSAKTQLDKEIKKNKGKHKLAIAL
                     DLDETVLDNSPYQGYASIHNKPFPEGWHEWVQAAKAKPVYGAKEFLKYADKKGVDIYY
                     ISDRDKEKDLKATQKNLKQQGIPQAKKSHILLKGKDDKSKESRRQMVQKDHKLVMLFG
                     DNLLDFTDPKEATAESREALIEKHKDDFGKKYIIFPNPMYGSWEATIYNNNYKASDKA
                     KDKLRKNAIKQFDPKTGEVK"
     gene            5492..6541
                     /locus_tag="O0293_002122"
     CDS             5492..6541
                     /locus_tag="O0293_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HCY0280995.1"
                     /translation="MFLAWNEIRRNKLKFGLIIGVLTMISYLLFLLSGLANGLINMNK
                     EGIDKWQADAIVLNKDANQTVQQSVFNKKDIENKYKKQATLKQTGEIVSNGHQKDNVL
                     VFGVEKSSFLVPSLIEGHKATKDNEVLADETLKNKGFKIGDTLSLSQSDEKLHIVGFT
                     ESAKYNASSVIFTNDATIAKINPRLTGDKINAVVVRDTNWKDKKLNQELEAVSINDFI
                     ENLPGYKPQNLTLNFMISFLFVISATVIGIFLYVMTLQKTSLFGILKAQGFTNGYLAN
                     VVISQTVILALFGTAFGLLLTGVTGAFLPDAVPVKFDVLTLLVFAIVLMIVSVLGSLF
                     SILTIRKIDPLKAIG"
     gene            6554..7231
                     /locus_tag="O0293_002123"
     CDS             6554..7231
                     /locus_tag="O0293_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0280996.1"
                     /translation="MLKFENVTKSFKDGNRNIEAVKDTNFEINKGDIIALVGPSGSGK
                     STFLTMAGALQTPTSGHILINNQDITTMKQKALAKVRMSEIGFILQATNLVPFLTVKQ
                     QFTLLKKKNKNVMSNEDYQQLMSQLGLTSLLNKLPSEISGGQKQRVAIAKALYTNPSI
                     ILADEPTAALDTENAIEVIKILRDQAKQRKKACIIVTHDERLKAYCDRSYHMKDGVLN
                     LENETVE"
BASE COUNT         2748 a         1051 c         1260 g         2208 t
ORIGIN      
        1 aatgtaaagg tatcaaatag ttatttcata atttattatg atgacataat tttcagtaga
       61 tagttgtaaa ttaacactga aatagatgtt tatattaaga agactttaat atcataatta
      121 atcaaaatct tgcgtcaaac atttaaacga attttaagtt gataacaatt ggcaaaacat
      181 tgttcaaaca tcacaatgat aaagcatatt atcagtattg tagtgtgtgg aaaatgacag
      241 ccatctaagg agaaaaatga tgaaaagaat attggtagta tttttaatgt tagcaattat
      301 attggcaggt tgttctaata aaggtgaaaa gtatcaaaaa gatattgata aagtgtacaa
      361 agaacagaat caaatgaata aaattgcctc gaaagtacaa aacactatta aaacagacat
      421 taaacaagaa gacagtaata cacatgttta taaagatggt aaagtcattg ttattggtat
      481 tcaattatat aaagatcgtg aaaaaatgta ttatttcgca tatgaaataa aagatggtaa
      541 ggcagagatt aatagagaaa tagacccaat taagtatatg aaagaccata aagcagatta
      601 tgaagatgaa aatgtagaag tggaaaaaga ttaacatagg ctttctttaa aacttttaac
      661 cagtttcaaa aagtaaatga gctttagcga caataatttt taaaataaac acgatataaa
      721 tttaaacatt tatatataag aacacaaacg caagtttgta aataataata ataaaggagt
      781 aagacaatgg aaaaatcgat caaaataatg acaataatag gaattgttgt tcagggttta
      841 gcaacggtat ttagtttact attgatggtt ttagcagcat caggtgtaat gactacagat
      901 gtgtcaacaa cagttaatgg tgaggttgac ccagttgatg cagaaacagc agcagcaatt
      961 ttcactgtat tattcttatc cctattcata tttggaatca tttcaattat tttaggtgca
     1021 atcggtatgt ttaaagcatc taaaaacaaa aaaatgagtg gtatattgtt gattattgga
     1081 gctgtaataa gtggtaacat aattacattt gctttatggt taatcagtgg tattaaacta
     1141 cttactaata acaagcctaa agatgaaata agcgacttat cataaacatc gtatattgaa
     1201 atttcaagat ttcttaagta attaaataaa gtgctctctt agaagtagat tttcagtcaa
     1261 taactgcttt taagagagca ttttgtattt gataatgctc atagcatcgg ataaaaatca
     1321 ttgacgaact ttaaaaaaat acgttagaca atgcacttct aaatattaaa ggcatcgact
     1381 aacgcattgt tcaaatatag ttaattattt ttataaaatt gatgatgatc attcaagtaa
     1441 gcatagaata aacctataat gagtccgcct ccaatatagt taccgataaa agccgcagcg
     1501 atattcgaaa tagctggtat gaagtgcaat gtatcaactt gataaattaa accacccata
     1561 aataagcaac tgttgtaaac gacgtgttca taacccataa aggcgaatat ggtaacaccg
     1621 aacatcatga caaacatttt tgcgagtaca tcgtcaattt gcatggcaat aactaatgaa
     1681 atattgataa agaaattggc gaagatcgct ttcattaata tacttacaaa accagtagac
     1741 aacgttttat gctctataac tgctgataac tgatttaaca tatctggcgt cattacattt
     1801 gaaaaacgca tgaaactaaa taaaatagca gcacctaaaa tatttcctgc aaagcataat
     1861 aaaaatattt tcaatactct agttggttta attactttat aatacaggcc tacagtaaag
     1921 tacatgaagt tactggttag tagttcggag tttgtaaata aaatgagtac taacgcaaag
     1981 ctgaatgtaa tggcactggc catattcaca atgcctggcg gtaaatctgg ttcgtgtgtt
     2041 gctttaactg ataatacgaa gaccgtaata atcccgataa taaatcctgc catcatcgcg
     2101 cgtaataaat aacgttttaa ataaacgctt tgtaatatat cttttgttct tatcgtttcg
     2161 actacgttat ttacccagtc gtccccataa aaaatcttat cccatttaat atgtttctcc
     2221 ttcacaatga catcctcact tttcatttgt taatcagagt atactatgaa ttgccttatt
     2281 tgtgaataat ttcacaaaat taattttaaa aactaatatt tttgaataac agctaggatt
     2341 ttttaacaaa tcatttattt taaaataatc aatagacaga cggtatcact gaaaagtcat
     2401 gaatttcata taaaaaagcc tagtaacgat gggtcaatca ttactaggca gtgagcattt
     2461 attaagttgt cgcttgtttc ggacggcgta taaatacatc gattatgaaa ccgataatag
     2521 caaagagcat gaatggtaca agccaagcta aatcgatatc tgctaaaggt aacatcataa
     2581 acgatttcaa aataacaccg tgtaataagt tgaaactatt tagtatttgt aaaattgaaa
     2641 taatcaatgt aataacagtt gcgagtcgat aggcccaact gaatctgaat gtgctaaaca
     2701 tgttagcaaa tgatatgagt acaagtgcaa tcgacacggg atatattaaa gtcaataatg
     2761 ggacagcaat ttttaaaatc atttctaaac caagtgttgt aaataagaac cctatgatag
     2821 agaaaataag tgcgaatatt ttataagaaa acttaggtac gtgtttctta gtaaatgtgg
     2881 cgcaagcatt gacgagtcct atacatgttg ttaggcatgc aaggataacc gtcattccaa
     2941 atacgaggtt accgaacgaa ccaaataatc gtaatgagtt gtacgtcaat atatctgtac
     3001 catctttaaa gtttcctgga gctgttgatg ccccaacgta tgcaagtgca aagtaaatca
     3061 ttccaagtaa tatggctgca ataagacctg aaaagcagac atattttaaa attttcatgc
     3121 gatctgtgag gcctttaaac ttatagccat tgacaatgac tacggaaaaa gctaacgcag
     3181 caacaagatc cattgtaaaa tagccttcca aacttcctga aatgaaagga tgtgttatat
     3241 atttatcctt aggtgcactt agtgcagatt cagggttgaa aatgacagca atacttaata
     3301 gagcgaccat taatagtaat aacggtgtta ataatttacc taaattatca acgattttcg
     3361 atggatttaa actaatccag taaacgatgg caaaaaagat tgctgcgaat ataattaaag
     3421 tccattggtt atgcacaggt aaaatgtgtc ttgtaccaat ttcgtacgcg acatttgcag
     3481 cacgtggaat accgtaaaat gctccgatag acatgtaaat cacgacagca aaaataaacc
     3541 cgaaccatgg atgtatacga ttgcctacac tttcaacacc ttcatcataa aatgcaacaa
     3601 caataacagt aataaagggg agtaatatgc ctgtaagggc aaagcctagc ataccaatcc
     3661 acatattttg acccgctgta tggccaagca tgggcgggaa tattaaattt ccggctccaa
     3721 aaaatagtga aaataacatg aggcccgaaa taataacttg ttttttcaat gtaatttcct
     3781 tcctaaaact agatattcat aataatttaa aaaatctgaa aaataaaaac gttcttactt
     3841 tatctttaaa tgacaatact atgattctat tattttataa aaaagattgc aataataacg
     3901 tcttgctatt attggataat gacaattaaa gtgtcgtttt tatgaattaa aataggtgaa
     3961 agtaagttgg aaatacgttg gagcgttgtg atttttatga gaaattgtat gagtgaggac
     4021 tgctatttat aaaaggtaaa ctatggatga gtttatggtt gttccaatag gtaaataaga
     4081 gatagcacac tgtacacata atgatacgtg gatgagtgaa tatgtctgat aggaaggcct
     4141 agtcactcaa atgaatttaa gtttattacg tgataaatca caaatctctc tcatgtgata
     4201 ggtctcccat taaatcatgc ttatatgaaa tgttcacata tttgttagct tttcaagaaa
     4261 taatattaaa tatcttttca tagtatagca atattaaata aatgcattta taattttaac
     4321 tgtattttaa atattaatca tgaggtgata agatgaataa aatttcaaag tatattgcaa
     4381 tagcatcatt atcggtagcg gttacagttt cagcaccaca aacgacaaat tctacagcgt
     4441 ttgccaaaag ttctgctgaa gttcaacaaa cgcaacaagc ttctatacca gcatcacaaa
     4501 aggcgaatct tggtaatcaa aatattatgg cagtggcttg gtatcaaaat tcagctgaag
     4561 caaaagcatt atatttacaa ggttataaca gtgcaaagac acagttagat aaagagatta
     4621 aaaagaataa aggtaaacat aagttagcta ttgctttgga tttagatgaa acagttttag
     4681 ataattctcc atatcaaggc tatgcatcaa tacataataa acctttccca gaaggttggc
     4741 atgaatgggt acaagctgct aaagctaaac ctgtctatgg cgcaaaagaa ttcttgaaat
     4801 atgctgacaa aaaaggtgtc gatatctact atatttctga tagagataaa gaaaaagatt
     4861 taaaggcaac acaaaagaac ttaaaacaac aaggtatccc tcaagctaag aagagtcata
     4921 ttttactaaa aggtaaagat gataagagta aagaatcacg cagacaaatg gttcaaaagg
     4981 atcataaact tgtcatgcta tttggagata atttattaga ctttacagat ccaaaagaag
     5041 ctacagctga atctcgtgaa gcattaattg aaaaacataa agacgatttc ggtaagaaat
     5101 atatcatttt ccctaaccca atgtatggta gttgggaagc tacgatttac aacaataact
     5161 ataaagcaag tgacaaagca aaagataaat tacgtaaaaa tgctattaag caattcgatc
     5221 ctaaaacagg cgaagttaaa taatatatga attggacgtc tacatgtact taaaggtata
     5281 tgtaggcgtt tttaaattga tccttaattt taaatgtacc gtacaaaaat aatagacaat
     5341 taattgcatt ttccataaat ttgcttttaa tataaaaagt tcgggtataa tgaaggataa
     5401 gtgtatgtat aacattacag agcgcaacac gacgtacgtg catctaagaa tataaacaat
     5461 gtttaaatca tagtagtagg gagagaaatg catgttttta gcttggaatg aaatacggcg
     5521 caacaaattg aagtttggac taattattgg tgtgttaacg atgattagtt acttgctatt
     5581 tttattatct ggattggcga atggtcttat caatatgaat aaagaaggca ttgataagtg
     5641 gcaagcagat gccattgttc taaataaaga tgccaatcaa actgtgcaac aatctgtttt
     5701 taacaagaaa gatattgaaa ataaatacaa gaagcaagct actttgaagc aaacagggga
     5761 aattgtgtct aatggccatc aaaaagacaa tgttttagtg ttcggtgttg aaaagtcatc
     5821 atttttagtt ccgagtttaa tagaagggca taaagcgact aaagataatg aagtgttagc
     5881 tgatgaaacg cttaaaaata aaggatttaa aattggtgac acattatcac tatctcaatc
     5941 agatgaaaaa ttgcatatcg taggttttac agaaagtgca aaatataatg cgtcatcagt
     6001 cattttcacg aatgacgcta ccattgccaa gatcaatcct agattgactg gagataaaat
     6061 taatgcagtt gttgtacgtg atacaaattg gaaagacaaa aaattaaacc aagagcttga
     6121 agcggtaagt attaatgact ttattgaaaa tttaccaggt tataaaccac agaacttaac
     6181 attaaacttt atgatttcat tcttatttgt catttcagct acagttatag gcattttcct
     6241 atatgtcatg acattacaaa agacgagttt atttggcata ttaaaagctc aaggatttac
     6301 gaatggctat ttggcgaatg tggtaatttc gcagacggtc atattagcac tatttggtac
     6361 ggcatttggc ttactgttaa caggcgttac aggtgcattt ttacctgatg cagtacctgt
     6421 caaattcgat gtactaacat tgctcgtatt tgcaattgtg ttaatgattg tctctgtatt
     6481 aggaagttta ttctccattt taacaattag aaaaatagat ccgttaaagg cgattgggta
     6541 ggaggtgtag caaatgttga aatttgaaaa tgtaacaaag tcatttaaag atgggaatcg
     6601 taacattgaa gcggttaaag atacaaattt tgagataaat aaaggtgata ttatagcatt
     6661 ggttggacct tctggctctg gtaaaagtac atttctaact atggcaggtg ctttacaaac
     6721 accgacatct gggcacattt taatcaataa ccaagatatt acgacaatga agcaaaaagc
     6781 attggcaaaa gttagaatgt ctgaaatagg ttttatttta caagctacaa accttgtacc
     6841 atttttaacg gtaaagcaac aatttacatt attgaaaaag aaaaataaga atgttatgtc
     6901 taatgaagac tatcagcaac ttatgtcaca attaggtcta acttcattgc ttaataagtt
     6961 accttcagaa atttcaggtg gtcagaaaca acgtgtggcg atagccaaag cgttatatac
     7021 gaatccgtcg attattttag cggatgaacc taccgcggcg ttagatactg aaaatgcgat
     7081 tgaagtcatt aaaattctac gtgatcaagc caaacaaaga aagaaagcat gtattattgt
     7141 tacacatgat gaacgactta aagcatattg tgatcgttca tatcatatga aagatggcgt
     7201 ccttaatctt gaaaatgaaa cagtagaata gttttattaa gccggtacat catgtgccgg
     7261 tattttt
//
LOCUS       DAKTIO010000136         7241 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.136, whole genome shotgun sequence.
ACCESSION   DAKTIO010000136 DAKTIO010000000
VERSION     DAKTIO010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7241
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.136"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..114
                     /locus_tag="O0293_002124"
     CDS             <1..114
                     /locus_tag="O0293_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4909 domain-containing protein"
                     /protein_id="HCY0280997.1"
                     /translation="ALQDMNRIPIDIADDVATMGKSPHQNDRMTLKLNKNN"
     gene            273..1751
                     /gene="menE"
                     /locus_tag="O0293_002125"
     CDS             273..1751
                     /gene="menE"
                     /locus_tag="O0293_002125"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCY0280998.1"
                     /translation="MDFWLYKQAQQNGHHIAITDGQESYTYQNLYCEASLLAKRLKAY
                     QQSRVGLYIDNSIQSIILIHACWLANIEIAMINTRLTPNEMTNQMKSIDVQLIFCTLP
                     LELRGFQIVSLDDIEFAGRDITTNSLLDNTMGIQYETSNETVVPKESPSNILNTSFNL
                     DDIASIMFTSGTTGPQKAVPQTFRNHYASAIGCKESLGFDRDTNWLSVLPIYHISGLS
                     VLLRAVIEGFTVRIVDKFNAEQILTMIKNERITHISLVPQTLNWLMQQGLHEPYNLQK
                     ILLGGAKLSATMIETALQYNLPIYNSFGMTETCSQFLTATPEMLHARPDTVGMPSANV
                     DVKIKNPNKEGHGELMIKGANVMNVYLYPTDLTGTFENGYFNTGDIAEIDHEGYVMIY
                     DRRKDLIISGGENIYPYQIETVAKQFPGISDAVCVGHPDDTWGQVPKLYFVSESDISK
                     AQLIAYLSQHLAKYKVPKHFEKVDTLPYTSTGKLQRNKLYRG"
     gene            1756..2757
                     /gene="menC"
                     /locus_tag="O0293_002126"
     CDS             1756..2757
                     /gene="menC"
                     /locus_tag="O0293_002126"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCY0280999.1"
                     /translation="MKLTALHFYKYSEPFKSQIVTPKVTLTHRDCLFIELIDDKGNAY
                     FGECNAFQTDWYDHETIASVKHVIEQWFEDNRNKSFETYEAALKLVDSLENTPAARAT
                     IVMALYQMFHVLPSFSVAYGATASGLSNKQLESLKATKPTRIKLKWTPQIMHQIRVLR
                     ELDFHFQLVIDANESLDRQDFTQLQLLAREQVLYIEEPFKDISMLDEVADGTIPPIAL
                     DEKATSLLDIINLIELYNVKVVVLKPFRLGGIDKVQTAIDTLKSHGAKVVIGGMYEYG
                     LSRYFTAMLARKGDYPGDVTPAGYYFEQDVVAHSGILKEGRLEFRPPLVDITQLQPY"
     gene            complement(2754..3011)
                     /gene="yidD"
                     /locus_tag="O0293_002127"
     CDS             complement(2754..3011)
                     /gene="yidD"
                     /locus_tag="O0293_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCY0281000.1"
                     /translation="MKKIFLAMIHFYQRFISPLTPPTCRFYPTCSEYTREAIQYHGAF
                     KGLYLGIRRILKCHPLHKGGFDPVPLKKDKSASKHSHKHNH"
     gene            3077..3550
                     /gene="ytkD"
                     /locus_tag="O0293_002128"
     CDS             3077..3550
                     /gene="ytkD"
                     /locus_tag="O0293_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase YtkD"
                     /protein_id="HCY0281001.1"
                     /translation="MKFRDKDNRQVNLTFKKDNEIADGNHVLAIPTFKNQLLFTKHNL
                     RGIEFPGGKRERGESSAEAVTRELYEETGAKVKNIYYIAQYTIETHDQTDFVKDVYFI
                     EVESLVSKNDYLETAGPVLFNCINDIELAQRSFLLQDSTILKCVERVQSLGFYQT"
     gene            3555..4301
                     /locus_tag="O0293_002129"
     CDS             3555..4301
                     /locus_tag="O0293_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HCY0281002.1"
                     /translation="MPIEYFTHQFEEITYLSDDLQVKALMMTPHHEVNRIVVYLRGGK
                     GQVGRVRAGRLMQFSDSQTLVIGPYYRGNNGSEGKDEFYRGDLNDVTQLLRLLHDKYP
                     HAFIHMVGFSRGGLQGLLTFQDLPVTSYTIWGGVSDIDLMYEERVDLRGMLRRMIGHP
                     KKDRAAYEARQAIPNINENSPPILIVHGGKDQQVGIHHAYYLADQLELKGATHETFYQ
                     MAEGHVPRPPAMVETLTYIKEFMNQVESHS"
     gene            4676..5161
                     /gene="tnpA"
                     /locus_tag="O0293_002130"
                     /pseudo
     CDS             4676..5161
                     /gene="tnpA"
                     /locus_tag="O0293_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500412.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(5297..6889)
                     /gene="pckA"
                     /locus_tag="O0293_002131"
     CDS             complement(5297..6889)
                     /gene="pckA"
                     /locus_tag="O0293_002131"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCY0281003.1"
                     /translation="MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTE
                     LGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEINQPIDEETFLKLYHKVLDYLDK
                     KDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVS
                     APHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMS
                     MHCSANVGEKGDVALFFGLSGTGKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAK
                     AINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENTRAAYPINHIDNIVV
                     PSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSF
                     STCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMV
                     NQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTILNPINAWSDKEKYKAQAEDLIQRF
                     EKNFEKFGEKVEHIAEKGSFNK"
     regulatory      7044..7154
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT         2421 a         1156 c         1420 g         2244 t
ORIGIN      
        1 gcattacaag acatgaaccg tataccaatt gacattgcag atgatgtggc aacaatgggt
       61 aaatcaccac atcaaaatga cagaatgaca ctaaaattaa ataaaaataa ctgataatag
      121 tagacgaatg atataacgat atgtattgag cattataaca atcatatcaa tgcatcaatt
      181 cgtctacttt tttatatttt taaacataac cactgctaga atgtcataaa atcgttataa
      241 ttataatgta aatgattttg gaggaataac gcatggactt ttggttatat aaacaagcac
      301 aacaaaatgg acatcatatt gcgataacag acggtcaaga atcttatact tatcaaaatt
      361 tatactgtga agcgagtcta ttggctaaaa gactcaaggc ttatcaacaa tctcgtgttg
      421 ggctatacat agataattcg attcaatcga tcattttaat acatgcatgt tggttggcaa
      481 atattgaaat tgcgatgatt aatacaaggt tgacacctaa tgagatgacg aatcagatga
      541 agtcaatcga tgtacaattg attttttgta ccttgccact ggaattacga gggtttcaaa
      601 ttgtatcgct ggatgatatt gaattcgctg gaagggatat tacaacgaac agtttgttgg
      661 acaacacaat gggtatccaa tatgaaacat cgaatgaaac tgtggtgccg aaagagtcgc
      721 cgtccaacat attaaataca tcatttaatt tagatgacat tgcatcgatt atgtttacat
      781 cagggacaac gggccctcaa aaagcggtgc cacaaacgtt tcgtaatcat tatgccagtg
      841 caatcggatg taaagagagc ttgggatttg atcgtgacac taattggcta tctgtcttgc
      901 cgatttatca tatttcgggt ctcagtgtac ttttaagagc tgttattgaa ggttttactg
      961 tgcgcattgt tgataaattc aatgccgaac aaattttaac gatgattaaa aatgaacgaa
     1021 tcacgcacat ttcgcttgtg ccacaaactt taaattggct tatgcaacaa ggtttacatg
     1081 aaccttataa tttacaaaaa atattactcg gcggtgctaa attatctgcc actatgatag
     1141 agacggcatt acaatataac ctgccaattt ataattcatt tggtatgact gagacatgtt
     1201 cgcaattttt aacagcaaca ccggaaatgt tgcatgcacg tcctgacact gtagggatgc
     1261 caagtgccaa tgttgacgta aaaattaaaa atcctaataa agaaggtcat ggagaattaa
     1321 tgattaaagg cgccaatgtg atgaatgtat atttgtatcc aacagattta acgggtacgt
     1381 ttgaaaatgg ttattttaat acgggtgaca ttgctgaaat agatcatgaa ggttatgtca
     1441 tgatttatga ccgacgtaaa gatttaatta ttagtggcgg tgaaaatatt tatccatatc
     1501 aaattgaaac ggtagcgaag caatttccag gtatcagtga cgcagtatgt gtagggcatc
     1561 ctgatgatac ttggggccaa gtgcctaaat tatattttgt cagtgaaagt gacatttcaa
     1621 aggcacaatt gattgcatat ttatcacaac atttagcgaa atataaagta ccgaaacact
     1681 ttgaaaaggt tgatacttta ccttatacat caacaggtaa attacaaaga aataagttgt
     1741 atagaggatg atttgatgaa gttgacagct ttgcattttt ataaatatag tgagccattt
     1801 aagtcacaaa ttgtaacacc gaaagtcact ttaacgcatc gtgattgttt gtttatcgaa
     1861 ttgattgatg acaaaggaaa tgcatatttc ggggaatgta acgcttttca aacagattgg
     1921 tatgatcatg aaacaattgc ctcagtgaaa catgtaattg agcaatggtt cgaagataat
     1981 agaaataaat catttgaaac gtatgaagca gcactaaaat tagtagattc attggaaaat
     2041 acgcctgctg caagggcaac tattgtcatg gcattgtatc aaatgtttca tgtactgcct
     2101 tcattttcag tagcatatgg agcgacagcg agcggcttat caaataaaca actagagtca
     2161 ttaaaagcaa caaagccgac cagaattaaa ttaaaatgga cgcctcaaat catgcatcaa
     2221 attagagtgt tacgggaatt agactttcac tttcaattgg ttatagatgc aaatgagtcc
     2281 ttggatcgtc aagatttcac acagttacag ttattagcgc gtgaacaggt tctttatatc
     2341 gaagagccat ttaaagatat ctccatgctt gatgaagtag cggatggaac aatacctcca
     2401 atagcccttg atgaaaaggc aacatcgttg ttggacataa ttaatttgat agaattatac
     2461 aatgtgaaag ttgttgtatt aaaaccattt cgtctaggtg gcattgataa agtgcaaacg
     2521 gcaattgata ctttgaaaag tcatggtgca aaagtggtta ttggcggcat gtacgaatat
     2581 ggtttaagcc gctattttac agcgatgctt gctcgtaaag gtgattatcc aggagatgtt
     2641 acaccagcag gttattattt tgaacaagat gtggtggcgc attcaggcat attaaaagag
     2701 gggcgactcg aatttcgccc ccctttagtg gatataactc aattacaacc atattaatgg
     2761 ttatgtttat gtgaatgctt gcttgctgac ttgtcttttt ttaacggaac agggtcaaag
     2821 ccgcctttat gaagcggatg acattttaaa atacgacgga tacctaaata aaggcctttg
     2881 aaagcaccgt ggtattgaat cgcttctcta gtgtactctg aacatgttgg ataaaaacga
     2941 caagttggtg gagtgagtgg cgaaatgaaa cgttgataaa aatgaatcat cgccaagaat
     3001 atctttttca taattaatgc ctccaacgat agtaattaac tattatatta acatacaatg
     3061 tagggatgtg atgcgcgtga agtttaggga taaagataat cgtcaagtta atttgacatt
     3121 taaaaaggat aatgagatag cagatggcaa tcatgtgcta gctattccaa cgtttaaaaa
     3181 tcaattgctt tttaccaaac ataatttacg ggggattgaa tttcctggtg gtaaaaggga
     3241 acgcggggaa agtagtgctg aagcagttac acgtgaatta tatgaagaaa caggcgccaa
     3301 agttaaaaat atttattaca tagcacaata taccattgaa acacatgatc aaactgattt
     3361 tgtaaaagat gtatatttta tcgaagttga atcattggta agcaagaacg attatttaga
     3421 aacagcaggg ccagtgttgt ttaactgtat caatgatatt gaactcgcac aacggagttt
     3481 cttactgcaa gatagtacca ttttaaaatg cgtagagagg gtgcaatcac ttgggtttta
     3541 tcaaacgtaa acgaatgcca attgaatatt ttacgcatca attcgaagaa attacatatt
     3601 tgtcagatga cttacaagta aaagctttaa tgatgacacc acatcatgaa gtaaatcgca
     3661 ttgtagttta tttgcgaggt ggcaaaggtc aagtaggacg tgtgcgcgca ggtagattaa
     3721 tgcaattttc agatagtcaa actttagtga ttgggccata ctaccgaggt aacaatggga
     3781 gtgaaggcaa agacgaattt taccgtggcg atttaaatga tgtgacgcaa ttattaaggt
     3841 tgctacatga taaatatcca catgcattta tccacatggt tggtttttcg cgaggtggtt
     3901 tacaaggatt gttgacattt caagacttac cagtaacaag ttatacaatc tggggtggtg
     3961 tctcagatat tgatttaatg tatgaagaac gtgtcgattt aagaggcatg ctacgaagaa
     4021 tgattggtca tccgaaaaaa gatcgagcgg catatgaggc acgccaagcg attccaaaca
     4081 ttaatgaaaa cagtccgcca atattaattg tacatggagg gaaagaccag caagttggta
     4141 ttcatcatgc gtattattta gcggaccaac tagagttaaa aggtgccacg catgaaacat
     4201 tttatcaaat ggcagaagga catgtgccaa gaccaccagc gatggttgaa acattgactt
     4261 atattaaaga gtttatgaac caagttgagt cgcatagcta gtatgaaacg tggtgtgcgc
     4321 atgggaacta tcaagctttg aagatgtggt actacatgcg ttgaattagg gatcggtact
     4381 ttatatgaag acgtgctgct tccattaaat gagtgatgcg attttggcat gaaggtcacc
     4441 ttaaatgtac attgttgtaa taaaattgcc tataaatttt tagcacataa aataagagga
     4501 gccaaccatt gttagactat aacaacggtt ggctctttaa ttgtaaaaag aaaaccatac
     4561 gctatgcgta tggttcagaa aaggttctac cattgtcaca aaaaatgcat ctctacgtgc
     4621 tagaataaat attggtcagc caaccaaaat aatcaacacg aggagatgct atttaatgtc
     4681 atctgacaca aacagtttag cacatacaaa atggaattgt aagtaacata ttgtctttgc
     4741 acctaaatac agaagacaag tgatatatgg aaaaataaaa aaagatatag ggattatatt
     4801 gcgtcaatta tgtgaaagaa aaggtgtaga gataattgaa gcagaggcat gtaaagatca
     4861 tatccatatg ttagttagta taccacccaa acttggggta tcatcatttg ttggctattt
     4921 aaaaggaaaa agtagtttaa tgatatttga tagacatgct aacttaaagt atagatatgg
     4981 aaatagaaag ttttggtgta aaggatttta tgtggataca gtaggtagaa ataaaaaagt
     5041 gattgaaaat tatattcgta atcaattaca agaggatatc gttgcagacc aaatctcaat
     5101 ggaagaatat ttagatcctt tcactggaga gaaaaataaa aaaagaaaga aaaaagagta
     5161 accctttagg gttgctggaa tagtagtgca gttggctgac ttgtcagtgc ccttttaggg
     5221 ctggccagta aggaaggctt atagccgcag aacaaaccac ccgttcacac gggtggtttt
     5281 gatttagtat tcaaatttat ttgttgaagc tacctttttc agcaatatgt tcaacttttt
     5341 caccaaattt ttcgaagttc ttttcaaaac gttgaattaa atcttctgct tgtgctttat
     5401 atttctcttt gtcgctccaa gcattaattg gatttaaaat tgttttcggt acatcttcaa
     5461 tttctacagg aatgcttaaa ccaaacgtac tatcttttgt atattctgca tttttcaatt
     5521 tgccagaaat cgcttggttt accatttgac gtgtgtaatg taagctgatt ctacgtccta
     5581 caccatattt tccgccagtc catccagtat taacaagata aacatcaaca tcatgtaaat
     5641 cgataagttc acctaataga tcagcgtaaa cagtagggtg taacgggaag aacggtgctc
     5701 cgaaacatgt tgagaatgat ggttcaggtt ctgtcacacc acgctctgta ccagctaatt
     5761 tagaagtgaa accactcaag aaatgataca ttgcttggtc tttatttaac tttgaaatcg
     5821 gtggaataac accaaatgca tccgcagtta agaaaataat tgtatttgga tgtgctgctt
     5881 tagatggtac tacaatattg tcaatgtgat taattggata agcggcacgc gtgttttctg
     5941 tataacgatt gtcttcaaag tccactgaac catcttctgc aactacagtg ttctctaaaa
     6001 ttgcaccata tttgattgcg tcaaaaatct gtggttcttt ttctttggaa agattaattg
     6061 cttttgcata gcagccacct tcgatattaa agaccccgtt tttattccag ccgtgttcat
     6121 catcaccgat tagtttacgg tgtgggtcag ccgataaggt tgttttacca gtgccagata
     6181 gaccaaagaa taatgcaaca tcgccttttt caccaacgtt tgctgagcaa tgcatgctca
     6241 taatatcttg catcggtaag agataattca ttacagagaa gatacctttt ttcatttcac
     6301 cagcgtattc agtaccgccg attaaaatga ctttgtgttt aaatgaaata atgacaaagg
     6361 tttcagattt agtaccatca acttctggat ctgctttaaa atgtggtgca gaaacgatag
     6421 tgaagttagg tttaatcttt gtagcttctt cttttgattc aggtctaata aacatatttt
     6481 tagcaaataa attatgccat gctaattcat tgatgactgt aagttttaac attgtatctt
     6541 tatcgctacc agcgtagcct ttaaatacgt atagttcatc ttttttatct aaatagtcta
     6601 gtactttatg gtataacttc aagaaagttt cttcatcgat aggttgatta atttctcccc
     6661 aatcaatgtt atctctatat gaaggttcag agacaaaaaa tttgtcttta ggcgaacgac
     6721 cagtatattt tccagtactt gcattaacag caccaagttc tgttaatacc ccttcgttat
     6781 tgtctaagat tttattataa agttgtgtcg tcgaaagttg aaaatgtgac gttggttttt
     6841 tcagtaattt gtcaatttta gttgtttcag tgtatgtgtc tactgacatc ctaaatccct
     6901 ccaaagcgat aatttaaatg taagccttta catttaatta gtataacaca ttaacataat
     6961 taatctacaa tattttaccg taaatttatt tgtgaaaaat tgacattaat tcattaatta
     7021 ggtactatta tttttaacgg attctcttat cctgagtggt ggagggacat ggacccaatg
     7081 aaacccagca acctcttttt tataaaagaa aggtgccaaa ccgtttgcag acaaataggt
     7141 ctgaacgata agagcgaatg gacgtattag gccttctctc tatattaata gttagaaggt
     7201 cttttttatt tagctcacag agagagaatt ttcgtaatat a
//
LOCUS       DAKTIO010000137         7032 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.137, whole genome shotgun sequence.
ACCESSION   DAKTIO010000137 DAKTIO010000000
VERSION     DAKTIO010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7032
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.137"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            164..1033
                     /locus_tag="O0293_002132"
     CDS             164..1033
                     /locus_tag="O0293_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0281004.1"
                     /translation="MKKIFVIITTLLAVAIIIGSIIMVVFSQRQAQTFKIQQQQFVKK
                     PIPTLFLHGFGGSANSEKFMVKQAEKRGVTKDIITAYVSKDGAVTFKGKLRKDAVNPI
                     VKIELENNRQGYLDKNAAWFKNVLTKLQSEYNFDKFNFVGHSMGNLTFAQYMMTYGND
                     KSLPQLNKQVNIAGTFNGVLNMNEDVNEITVDKDGKPSRMNQPYQQLRVLKDIYKGKG
                     IEVLNIYGDLKDGTHSDGRVSNSSSKSLKYLLGNSPKSYRESKYEGEPAQHSQLHENE
                     NVANELIDFLWKK"
     gene            1316..2317
                     /locus_tag="O0293_002133"
     CDS             1316..2317
                     /locus_tag="O0293_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCY0281005.1"
                     /translation="MQNKQILFNKIPEGMPQEDTFKIEEIDTPKLESDGVLVQTLYIS
                     VDPYMRGRMTKADSYVQPFEIGKPIVSHVVAKVIDSTLADYKKGDVVVGMLPWRIINH
                     VQADQITKVPTTDVPLDLYLSVLGMPGQTAYHGLLDIGQPKAGDTVVVSAASGAVGSV
                     VGQIAKIKGCRVVGIAGGDKKVNYLTETLGFDAGIDYKKEDFAEALAQAVPDGIDVYF
                     ENVGGTVGDEVFKHLNRFARVPVCGAISSYNHPEADIGPRIQGTLIKKQVMMRGFLVS
                     EFANAFKEASEQLATWVQEGKIQSQVTIEDGFENAPHAFKNLFTGDNFGKQVIKVTE"
     gene            2551..3552
                     /locus_tag="O0293_002134"
     CDS             2551..3552
                     /locus_tag="O0293_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCY0281006.1"
                     /translation="MKMIGFEKPFKLEEGNLFKVYEQRKPTPENDDILVKVNSISVNP
                     VDTKQRQMEVTQAPRVLGFDAIGTVEAIGPDVTLFSPGDVVFYAGSPNRQGSNATYQL
                     VSEAIVAKAPHNISANEAVSLPLTGITAYETFFDTFKISTNPAENEGKSVLIINGAGG
                     VGSIATQIAKRYGLTVITTASRQETTEWCEKMGADIVLNHKEDLVRQFKEIPLVDYIF
                     CTYNTDLYYNTMIELIKPLGHITTIVAFNEDQDLNALKLKSITFTHEFMFARPIHRTP
                     DMIKQHEYLEDITKNIESGHYQPTTTQVFEGLSPENLYQAHQLLEKQSMIGKLVINI"
     gene            3859..5421
                     /locus_tag="O0293_002135"
     CDS             3859..5421
                     /locus_tag="O0293_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCY0281007.1"
                     /translation="MKNKSVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITTFIAL
                     HFSWYYLLLVLVILFVCVYILFSRYANITLGEEGEDPEFSLPSWFAMLFSAGMGIGLV
                     FWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFH
                     KGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFL
                     FNVPSNFTMQVILIVIATILFTWSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTL
                     YILNTFTNGLGNYIANFFSMSLRIPTGGQKFQWLQNWTIFYWAWWISWAPFVGIFIAR
                     VSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIADIAKAATETATFATLQ
                     HYPLGFVLSLITLLVIMIFFVTSADSATYVLGMLSSSGDINPKSFVKVSWGIIMALFA
                     IIMIYTGGTQAIQNLLIIAALPFSVVIIAMIWSLIKSLSEEKPRNSNKVLIKHRDPDI
                     LEYRSSKHIDEN"
     gene            5596..6132
                     /gene="amaP"
                     /locus_tag="O0293_002136"
     CDS             5596..6132
                     /gene="amaP"
                     /locus_tag="O0293_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCY0281008.1"
                     /translation="MKRLKNFILGLLIVVIVGFLLFMYIQDGRITEYQDYFLQFEWFQ
                     PLLISLAALLILIGLILVFSIFKPTHRKPGLYKDFDDGHVYVSRKAVEKTAFDTVAKY
                     DQVRQPNVVAKLYNKKNKSYIDIKTDFFVPNNVQVQSLTEAIRSDIKKNVEYFTEMPV
                     RKLEVNVRDQKTSGPRVL"
     gene            6145..6384
                     /locus_tag="O0293_002137"
     CDS             6145..6384
                     /locus_tag="O0293_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCY0281009.1"
                     /translation="MANNHNQNGQDSTQQVINFLKVFKWRIVGFLAFLLIAILFLTLG
                     FWKTVLIIVLCLIGVGIGYMKDRKQDFMNFLNRWS"
     gene            6447..6956
                     /locus_tag="O0293_002138"
     CDS             6447..6956
                     /locus_tag="O0293_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCY0281010.1"
                     /translation="MTVDNNKAKQAYDNQTGVNEKEREERQKQQEQNQEPQFKNKLTF
                     SDEVVEKIAGIAAREVKGILDMKGGLTDTFTNAFSSGNNVTQGVSVEVGEKQAAVDLK
                     VILEYGESAPKIFRKVTELVKEQVKYITGLDVVEVNMQVDDVMTQKEWKQKHEKNNEN
                     NNQERQGLQ"
BASE COUNT         2472 a         1000 c         1343 g         2217 t
ORIGIN      
        1 gtgacacacc attgaaagct aaagaagcaa tcaattaaca aacgttaacg gacaactttc
       61 caaagtcatc ttaaaagtta atgatgtatt caaaatcaac taaaggacat ttttaatcac
      121 atcctttata cttatataaa agacatgaat gggagttaaa agtgtgaaga aaatttttgt
      181 aattattacg acattgttag cggtcgctat tattataggc tctattatta tggtggtgtt
      241 tagtcaacga caggcacaaa catttaaaat acaacaacag caatttgtta aaaagccaat
      301 tccaacattg ttcttacatg gatttggtgg tagtgccaat tctgaaaagt ttatggtgaa
      361 gcaagcagaa aaaagagggg taactaaaga tatcatcact gcttacgttt caaaggatgg
      421 tgcagtgaca tttaaaggga aactaagaaa agatgcagtg aatcctattg tgaaaattga
      481 acttgaaaat aacagacaag gctatttgga taagaatgct gcgtggttta aaaatgtact
      541 gactaagtta caatctgaat acaatttcga taaattcaac tttgtcggtc actcaatggg
      601 gaacttaaca tttgcacaat atatgatgac atatggtaac gacaagtcac tgccacagtt
      661 aaacaaacaa gtgaatattg cagggacatt taatggtgtt ttaaatatga atgaagatgt
      721 caatgaaatt acagttgata aagatggtaa accaagtcga atgaatcaac cttatcagca
      781 attacgcgtg ctaaaagata tttataaagg taaaggtatc gaagtgctaa atatttatgg
      841 ggatttgaaa gatggtacac attcagacgg cagagtgtct aatagttctt cgaaatcatt
      901 aaaatattta ctgggaaata gtccgaaaag ttatcgagaa tctaaatatg aaggtgagcc
      961 tgcacaacat agtcagcttc atgagaatga aaatgttgct aatgagttaa tcgacttctt
     1021 atggaaaaaa taaagcaact gctagttttt ctggggcaaa aaagcttgta atattagagc
     1081 ttttcaaaaa ttatctattg tttgagagtt tccataaaat gtctaagtcg taacatttta
     1141 aatttatgat tatagcaaat ataatgtggt tgaagttaca tgaatgaatc agtgcttaag
     1201 tcaaaatata acaactaaga atgtatttgc atacagacat cacgaagaca caattcaaac
     1261 ttaacgctta agtaacaaat aagtaaaagt ttcaaataaa gaaaggtgat taatgatgca
     1321 aaataaacaa attcttttta acaaaatacc agaaggcatg cctcaagagg atacattcaa
     1381 aattgaagag attgacacgc ctaaacttga gagcgatggt gtacttgtac aaacgctgta
     1441 tatttcagtg gatccatata tgagaggacg tatgactaag gcagattcgt atgtacagcc
     1501 ttttgaaatt ggtaaaccga ttgttagcca tgtcgttgca aaagtaattg attcgacact
     1561 cgcagattat aaaaaaggag atgtcgttgt aggtatgttg ccttggcgaa tcattaatca
     1621 tgtccaagcg gatcaaatta ctaaagttcc aacgacagat gtaccacttg atttatattt
     1681 aagtgtttta ggaatgccag gacaaacagc atatcatggt ctgcttgata ttggacaacc
     1741 taaagccggt gatactgttg tggtttcagc tgcatctggt gctgtaggtt cagttgtagg
     1801 tcaaattgct aagattaaag gttgccgtgt agtaggtatt gcgggtggcg acaaaaaagt
     1861 aaattattta acagagacac tgggctttga cgctggtatt gattataaga aagaggattt
     1921 tgcagaagcg ttagcacaag cagtaccaga tggtattgat gtttattttg aaaatgttgg
     1981 tggaacagtt ggcgatgaag tatttaagca tttaaatcgt tttgcacgtg taccagtatg
     2041 tggcgcaatt tcgtcatata atcatcctga agccgatatc ggaccacgta ttcaaggtac
     2101 gcttattaag aagcaagtaa tgatgagagg cttcttggta agtgaattcg ctaatgcttt
     2161 taaagaagct agtgaacagc ttgcaacatg ggttcaagaa ggtaaaattc agtcacaagt
     2221 aaccattgaa gatggttttg aaaatgcgcc acatgcattt aaaaatttat ttactggcga
     2281 taactttggt aaacaagtta ttaaagtaac ggaataataa agtatacgtt ttaacaaact
     2341 aactttgtta attgaccaaa atttaataat tttttatcta aagtaaattt gttcagcaac
     2401 atggtagaaa taagtctaaa atttaatacg aaagtcgtct gtaattattt atatattcaa
     2461 atgaaaaagg agtcatgatg ctgcgatata agcacacatg gcttctattt tgtataataa
     2521 gaaataagtt cataaatgag gtgaaacgat atgaaaatga taggatttga gaagcccttt
     2581 aaattggaag aaggaaattt atttaaagta tacgaacaaa gaaagccaac gccagaaaac
     2641 gatgatatct tagtcaaagt aaatagcatt agtgtaaatc ctgtagatac aaagcaacga
     2701 caaatggaag tcactcaagc acctcgtgta cttgggtttg atgctattgg tactgttgaa
     2761 gctatcggac cagacgttac attatttagt ccgggtgacg ttgtatttta tgctggatcg
     2821 ccaaatagac aaggatcaaa tgcgacatat caattggtgt cagaagctat agttgcaaaa
     2881 gcaccacaca acataagtgc taatgaagcg gttagtctac cgctaactgg tattacggca
     2941 tatgaaacgt tttttgatac atttaaaatt tctacgaatc ctgctgaaaa cgaagggaag
     3001 tctgtcttaa tcattaatgg tgccggtggt gtaggcagta tagccactca aattgctaaa
     3061 cggtatggtt taactgtcat tacaacagca tcacgacaag aaacaacaga gtggtgtgaa
     3121 aagatgggcg cagatatcgt attgaatcat aaagaggatt tagtgcgtca atttaaagaa
     3181 attccattag tagattatat attttgcacc tataatacag acttgtatta caatactatg
     3241 attgaactga taaaaccatt agggcatatt acgactatcg tagcttttaa tgaagatcaa
     3301 gatttaaatg cattaaaatt aaaaagtata acatttacac atgaatttat gtttgcgcgt
     3361 ccaattcatc gaacgcctga tatgattaaa caacatgagt atttagaaga tattacgaaa
     3421 aatatagaat cagggcatta ccaaccaaca acgacacaag tttttgaagg tttatcacct
     3481 gaaaacttat atcaagcaca tcaattgttg gaaaaacaat caatgattgg aaaattagta
     3541 attaatatat agattagtaa gtaaagtcgc cttatgcaga aaatactgta taggcgactt
     3601 attttttgtg tctagcgact attgattcta aacggaaata tggcaacttt gcaggtatca
     3661 aatcaataat gcactagaaa ggcattactt aaatacaata aaatactttg ttgctcaatg
     3721 ataaataatt tgagtgtgtt ttatatatca tttttaaaag atgaggcatt attaattgtg
     3781 tctatataaa attacaaatg attaaaatgt tgcagttatg ggtatgaact taccattaca
     3841 cagaaagggt ttgtatttat gaaaaataaa tcagtaagtc tagtgttttg ggtatcttta
     3901 gtgatatgta cgatatttgt tgcttttgga gcaatatttc cgaaacagtt agaaaaactc
     3961 acgcaaaaca ttactacatt tattgcgcta catttttctt ggtattattt attgctcgtt
     4021 ttagttattt tatttgtctg tgtgtatatt ttattttcga gatacgcaaa cattacatta
     4081 ggtgaagaag gcgaagatcc tgaattctca ttgccgtctt ggtttgctat gttatttagt
     4141 gctggtatgg gaataggact tgtgttctgg acaacggcag aaccaatcag tcatgccttc
     4201 aaattaacgc ctattcataa agctggcacg caatctgcaa ttaatgatgc aatgcagttt
     4261 tcatttttcc attgggggat tcatgcatgg gcagtctatg gtattgtcgc gctagtgttt
     4321 gcatatttta gctttcataa aggatatcct ggcttagtca gcgctacatt gacgccttta
     4381 ttaggtgaaa aggcaatgcg tggaccttta ggtggtgcca ttgatgtatt ggcagtcata
     4441 gctacagtaa caggcgttgc tgcaacatta ggtttcggtg cattgcaaat aaacgaaggt
     4501 ttacattttt tatttaatgt accgagtaac ttcacgatgc aagtaatctt gattgtcatt
     4561 gcaaccatat tatttacttg gtcagcatgg tcaggtatcg ataaaggtat caaaacgttg
     4621 agtaatatca acatgttact tgcttttgta gtacttattg gtctttttat tgttgggcca
     4681 acgctataca ttttaaatac atttacgaat ggtctaggta attatattgc aaatttcttt
     4741 agtatgagtt tacgcattcc gacaggtggt caaaaattcc aatggttaca aaattggaca
     4801 atcttttact gggcatggtg gatatcttgg gcaccatttg taggtatatt tattgcacgt
     4861 gtgtcaaaag gacgtacgat taaagaattt attctaggtg tactttttgt cccggcgtta
     4921 gtttgcttta tattctttgc ggtattcggc gcgtcagcaa tttacttaca agataatcat
     4981 attgccgata ttgctaaagc tgcgacagaa acagcaactt ttgcgacgct acaacattat
     5041 ccgctaggtt ttgtattaag cttaattact ttacttgtta ttatgatttt ctttgttaca
     5101 tcagcggatt cggcaactta tgttttaggt atgttgagtt caagtggtga tattaatccg
     5161 aagtcatttg tcaaagtgag ttggggtatc attatggcat tatttgcaat catcatgatt
     5221 tacacaggtg gtactcaagc aattcaaaat ttattaatca ttgctgcgtt accattttcc
     5281 gtggtcatta tagcgatgat atggtcgctt ataaaatctt tgagtgagga gaaaccgaga
     5341 aatagtaata aggttttaat taaacatcgt gacccagata ttttagaata tcgttcttca
     5401 aaacatattg acgaaaatta attaataatg aggttttatt tacaaaaact agggaatata
     5461 ttactaaatg gtacttcatc ttatatatta aaaataaatt taaagttatt aatttattat
     5521 gtttaagaaa aaaactgatg ggtacatatt taatatgttt ttaaattaac tttaactaag
     5581 gaggtgtcgt taggggtgaa acgacttaaa aactttatcc ttggcttatt aatagtagta
     5641 atcgtggggt tcctgttatt catgtatatc caagatggac gtataactga atatcaagac
     5701 tacttccttc aatttgaatg gttccaacca ttacttattt cacttgctgc actattgata
     5761 ttaataggtc ttatattagt atttagtatc ttcaaaccta cgcatcgaaa acctggatta
     5821 tacaaagact tcgatgatgg tcatgtttac gtatctcgta aagctgttga aaagacggca
     5881 ttcgatacgg ttgcaaaata tgatcaagta agacaaccaa atgtagttgc gaaactatat
     5941 aacaaaaaaa ataaatctta tatcgacatt aaaactgact ttttcgtacc aaataatgtt
     6001 caagttcaat ctttaactga agcaattcgt tcggatatta aaaagaatgt agaatatttc
     6061 acagaaatgc cagtacgtaa gttagaagtt aatgtgagag atcagaaaac ttctggtcca
     6121 cgagtgttgt aagggaggta tatcatggct aacaatcata accaaaacgg acaagactct
     6181 acacaacaag tgattaattt tttaaaagta tttaaatgga gaatcgttgg tttcctagcg
     6241 tttctattaa tcgcgatatt attcttaacg ttaggttttt ggaaaacagt acttatcatc
     6301 gttttatgtt taattggtgt aggtattggg tatatgaaag accgtaagca agattttatg
     6361 aattttttaa atcgatggag ttaattcatc aatttagaat ttaattaaaa aaatataaaa
     6421 attaacaagt aaaaggagaa tctattatga ctgtagataa caataaagca aaacaagcat
     6481 acgacaatca aactggtgtt aacgaaaaag aaagagaaga gcgtcaaaaa caacaagaac
     6541 aaaatcagga gcctcaattc aaaaacaaat taacattctc tgatgaagtt gttgaaaaaa
     6601 ttgctggtat cgctgcacgt gaagttaaag gtatcttaga tatgaaaggt ggcttaactg
     6661 atacattcac taatgcattc tcaagtggca acaatgttac tcaaggtgta tctgttgaag
     6721 ttggtgaaaa acaagctgct gtagacttaa aagtaatctt agaatatggt gaatcagcac
     6781 caaaaatctt ccgtaaagta actgaattag ttaaagaaca agttaaatat attactggtt
     6841 tagatgtagt tgaagttaac atgcaagttg acgatgtaat gactcaaaaa gagtggaaac
     6901 aaaaacacga aaaaaataac gaaaacaata accaagaaag acaaggttta caataagtta
     6961 ttcgataaaa agggcttggg acattaaatt cttaggcaat gtaaaaaagc tgatttctat
     7021 taattatttg at
//
LOCUS       DAKTIO010000138         7029 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.138, whole genome shotgun sequence.
ACCESSION   DAKTIO010000138 DAKTIO010000000
VERSION     DAKTIO010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7029
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.138"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..526
                     /locus_tag="O0293_002139"
     CDS             <1..526
                     /locus_tag="O0293_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCY0281011.1"
                     /translation="LFKAITLKGPCYLHYYLQGYDEPMYTRQQVSLIEKLSQQQLFEY
                     EMNNLVTMMFELESGEYTILSKIIMKPTLLNQTYITYTKLLEQFTMEDIAAQQQVKIN
                     TIEDHVLEILIKGYMSNYDDYVELEDQLQFLNFYQQHRGERLKFYKEQFDTLSYFQLK
                     VLIVGFERGDLNVA"
     gene            516..1895
                     /locus_tag="O0293_002140"
     CDS             516..1895
                     /locus_tag="O0293_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCY0281012.1"
                     /translation="MLHDILRNKFGFESFKPGQQEIIESIMSQQHTLGILPTGSGKSL
                     CYQIPTYLSGKPTLIISPLISLMDDQVMQLKINGEKRVTCIHSGMDEIEKKHNIKCLR
                     HSRFIFLSPEFLLQPSNFKLISMIDFGMIVLDEAHCLSEWGYDFRPHYALIGKVTKHF
                     KEAVVLALTATAPPHLQDDLTEMLAIQFNVIKTTMNRPNISFKHLNFHDDEDKIEWLL
                     PFLQQSGPTIIYVSSKKMCLNLAQLIYDSGFLTGIYHGDMNYQERHTVQQQFLNNDIP
                     IIVATSAFGMGINKKDIRTIIHFHLSTSPSNYIQEIGRAGRDGELSQAISLFQPDDKY
                     ILETLLFADMITEEDVQNFEIGEFLAPDKQAVLTTLQSFYSIGALKQIFKQSFKRKQL
                     GFFRMIGYCKLDQCRRKYLLEFFGEYPPAQDRCCDNDSNITDIAILNKKKVIRSIGFD
                     EKLQNLFLR"
     gene            2048..3508
                     /gene="ebpS"
                     /locus_tag="O0293_002141"
     CDS             2048..3508
                     /gene="ebpS"
                     /locus_tag="O0293_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elastin-binding protein EbpS"
                     /protein_id="HCY0281013.1"
                     /translation="MSNNFKDDFEKNRQSIDTNSHQDHTEDVEKDQSELEHQDTIENT
                     EQQFPPRNAQRRKRRRDLATNHNKQVHNESQTSEDNVQNEAGTIDDRQVESSHSTESQ
                     EPSHQDSTPQHEEEYYNKNAFAMDKSHPEPIEDNDKHDTIKNAENNTEHSTVSDKSEA
                     EQSQQPKPYFTTGANQSETSKNEHDNDSVKQDQDEPKEHHNGKKAAAIGAGTAGVAGA
                     AGAMAASKAKKHSNDAQNKSNSGKANNSTEDKASQDKSKDHHNGKKGAAIGAGTAGLA
                     GGAASKSASAASKPHASNNASQNHDEHDNHDRDKERKKGGMAKVLLPLIAAVLIIGAL
                     AIFGGMALNNHNNGTKENKIANTNKNNADESKDKDTSKDASKDKSKSTDSDKSKEDQD
                     KATKDESDNDQNNANQANNQAQNNQNQQQANQNQQQQQQRQGGGQRHTVNGQENLYRI
                     AIQYYGSGSPENVEKIRRANGLSGNNIRNGQQIVIP"
     gene            3633..3749
                     /locus_tag="O0293_002142"
     CDS             3633..3749
                     /locus_tag="O0293_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281014.1"
                     /translation="MLFNKVFSRILAYQFNFLNSHQKDKTPQSTNLTRKNKK"
     gene            3910..4896
                     /locus_tag="O0293_002143"
     CDS             3910..4896
                     /locus_tag="O0293_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HCY0281015.1"
                     /translation="MQKVESIIIGGGPCGLSAAIEQKRKGIDTLIIEKGNVVESIYNY
                     PTHQTFFSSSDKLSIGDVPFIVEESKPRRNQALVYYREVVKHHQLKVNAFEEVLTVKK
                     MNNKFTITTTKDVYECRFLTIATGYYGQHNTLEVEGADLPKVFHYFKEAHPYFDQDVV
                     IIGGKNSAIDAALELEKAGANVTVLYRGGDYSPSIKPWILPNFTALVNHEKIDMEFNA
                     NVTQITEDTVTYEVNGESKTIHNDYVFAMIGYHPDYEFLKSVGIQINTNEFGTAPMYN
                     KETYETNIENCYIAGVIAAGNDANTIFIENGKFHGGIIAQSMLAKKQTPLES"
     gene            complement(5011..5979)
                     /locus_tag="O0293_002144"
     CDS             complement(5011..5979)
                     /locus_tag="O0293_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCY0281016.1"
                     /translation="MKHLLVIHTGGTISMSQDQSNKVVTNDINPISMHQDVINQYAQI
                     DELNPFNVPSPHMTIQHVKQLKDIILEAVTNKYYDGFVITHGTDTLEETAFLLDLILG
                     IEQPVVITGAMRSSNEIGSDGLYNYISAIRVASDEKARHKGVMVVFNDEIHTARNVTK
                     THTSNTNTFQSPNHGPLGVLTKDRVQFHHMPYRQQALENVNDKLNVPLVKAYMGMPGD
                     IFSFYSREGIDGMVIEALGQGNIPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAY
                     DGGGYQLAQQGFIFSNGLNGPKARLKLLVALSNNLDKAEIKSYFEL"
     gene            6056..6715
                     /locus_tag="O0293_002145"
     CDS             6056..6715
                     /locus_tag="O0293_002145"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCY0281017.1"
                     /translation="MKAINIALDGPAAAGKSTIAKRVASELSMIYVDTGAMYRALTYK
                     YLKLNKTEDFAKLVDQTTLDLTYKADKGQCVILDNEDVTDFLRNNDVTQHVSYVASKE
                     PVRSFAVKKQKELAAEKGIVMDGRDIGTVVLPDADLKVYMIASVEERAERRYKDNQLR
                     GIESNFEDLKRDIEARDQYDMNREISPLRKADDAVTLDTTGKSIEEVTDEILAMVSQI
                     K"
     gene            6746..6862
                     /locus_tag="O0293_002146"
     CDS             6746..6862
                     /locus_tag="O0293_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281018.1"
                     /translation="MQRLTHIQNECADNDLLKLKGDFYSMMNESCLFIFGKS"
BASE COUNT         2653 a         1081 c         1240 g         2055 t
ORIGIN      
        1 cttgtttaaa gcaataactt taaaagggcc atgttactta cattattatt tgcaaggcta
       61 tgatgaacca atgtatacga gacagcaagt tagtttaata gaaaagctat ctcaacagca
      121 attgtttgaa tacgaaatga ataatttagt gacaatgatg tttgaattag aaagtggaga
      181 atatactatt ttatcaaaaa taataatgaa acctacatta ttaaatcaaa cttatattac
      241 ttatacaaaa ttgcttgaac aattcacgat ggaagatata gcggctcaac aacaagttaa
      301 aatcaatact atcgaagatc atgtacttga aatcttaatc aaaggttaca tgtctaatta
      361 cgatgattat gttgaactag aagatcaact ccagtttttg aatttttatc aacagcatcg
      421 tggcgaacga ttaaaatttt acaaagaaca atttgacacg ttatcatatt ttcaattaaa
      481 agtattaatc gttggatttg aaagaggtga tctgaatgtt gcatgatatt ttacgaaaca
      541 aatttggatt cgagagtttt aaaccgggac aacaggaaat tatagaaagt ataatgtctc
      601 aacaacacac tctaggtata cttccaactg gaagtggaaa gagtttgtgt tatcaaatac
      661 ctacgtattt atcaggtaag ccgacattaa ttatctcacc gttaatatct ttaatggatg
      721 accaagttat gcagttgaaa ataaatggag aaaaacgtgt aacatgtatt cactctggta
      781 tggatgaaat tgagaaaaag cataatatta aatgtttacg acatagccgc ttcatctttc
      841 taagtccaga atttctcctg caaccgtcaa attttaaatt aatatctatg atagactttg
      901 gcatgattgt tctagatgaa gcacattgcc tatctgaatg gggatatgat ttcagaccac
      961 attatgctct aataggaaaa gtaacaaagc attttaaaga agcggttgtc ttagcattga
     1021 cagcaactgc accaccgcat ttacaagatg atttgacgga aatgttagcg attcaattca
     1081 atgttattaa aactacaatg aatcgcccaa atataagctt taagcatctt aattttcatg
     1141 atgatgaaga taaaattgaa tggttgctgc cgtttctaca acagtcggga ccaacgatta
     1201 tttatgtctc atcgaaaaag atgtgtctga atttagcgca acttatttat gattcaggtt
     1261 ttcttacagg tatttatcat ggtgatatga attatcaaga gcgacacaca gttcaacaac
     1321 aatttttaaa taatgatatt ccgattatag tcgcaacgag tgcttttggt atgggaatta
     1381 ataaaaaaga tattcgcaca atcattcact ttcatctttc aacaagtcct tctaactaca
     1441 ttcaagaaat tggccgtgcg ggtcgcgatg gtgaactaag tcaggcaatt agtttattcc
     1501 aaccggacga taaatatatt ttagaaacgt tattatttgc agatatgata acagaagaag
     1561 atgtacaaaa tttcgaaata ggagaatttt tagctcccga taaacaagcc gttttgacaa
     1621 cgttgcaatc attctatagt atcggcgcct tgaaacagat atttaagcaa tcatttaaac
     1681 gaaagcaatt aggattcttt cgcatgattg gctattgcaa attggatcaa tgtagacgga
     1741 agtatttatt agaatttttc ggtgaatatc caccggcaca agatcgatgt tgtgacaatg
     1801 attctaatat aactgatatc gcaattttaa ataagaagaa ggtaattaga agtattggat
     1861 ttgatgaaaa gttgcaaaat ttatttctca gatagtatta ctttactaaa agaaaattga
     1921 caagctataa ttagtgtata cacaattgaa aaatgattga aataattttg aaaaatatac
     1981 ataaacatat gtcatgtggg tatattttat gtaaaatcat tgtaatagaa tagaaaggaa
     2041 gatggctatg tctaataatt ttaaagatga ctttgaaaaa aatcgtcaat cgatagacac
     2101 aaattcacat caagaccata cggaagatgt tgaaaaagac caatcagaat tagaacatca
     2161 ggatacaata gagaatacgg agcaacagtt tccgccaaga aatgcccaaa gaagaaaaag
     2221 acgccgtgat ttagcaacga atcataataa acaagttcac aatgaatcac aaacatctga
     2281 agacaatgtt caaaatgagg ctggcacaat agatgatcgt caagtcgaat catcacacag
     2341 tactgaaagt caagaaccta gccatcaaga cagtacacct caacatgaag aggaatatta
     2401 taataagaat gcttttgcaa tggataaatc acatccagaa ccaatcgaag acaatgataa
     2461 acacgatact attaaaaatg cagaaaataa cactgagcat tcaacagttt ctgataagag
     2521 tgaagctgaa caatctcagc aacctaaacc atattttaca acaggtgcta accaatcaga
     2581 aacatcaaaa aatgaacatg ataatgattc tgtaaaacaa gatcaagatg aacctaaaga
     2641 acatcataat ggtaaaaaag cagcagctat tggtgctgga acagcaggtg ttgcaggtgc
     2701 agctggtgca atggctgctt ctaaagctaa gaaacattca aatgacgctc aaaacaaaag
     2761 taattctggc aaggcgaata actcgactga ggataaagcg tctcaagata agtctaaaga
     2821 tcatcataat ggcaaaaaag gtgcagcgat cggtgctgga acagcaggtt tggctggagg
     2881 cgcagcaagt aaaagtgctt ctgccgcttc aaaaccacat gcctctaata atgcaagcca
     2941 aaaccatgat gaacatgaca atcatgacag agataaagaa cgtaaaaaag gtggcatggc
     3001 caaagtattg ttaccattaa ttgcagctgt actaattatc ggtgcattag cgatatttgg
     3061 aggcatggca ttaaacaatc ataataatgg tacaaaagaa aataaaatcg cgaatacaaa
     3121 taaaaataat gctgatgaaa gtaaagacaa agacacatct aaagacgctt ctaaagataa
     3181 atcaaaatct acagacagtg ataaatcaaa agaggatcaa gacaaagcga ctaaagatga
     3241 atctgataat gatcaaaaca acgctaatca agcgaacaat caagcacaaa ataatcaaaa
     3301 tcaacaacaa gctaatcaaa atcaacaaca gcaacaacaa cgtcaaggtg gtggccaaag
     3361 acatacagtg aatggtcaag aaaacttata ccgtatcgca attcaatact acggttcagg
     3421 ttcaccggaa aatgttgaaa aaattagacg tgccaatggt ttaagtggta acaatattag
     3481 aaacggtcaa caaatcgtta ttccataata taactatata aattgtaact gaactgctat
     3541 atacaaacgt gcatattata agtatctcta aatattttgg agaacttaaa ttgcgcttat
     3601 aagtatgtag cggttttttc atttttcaaa gtttgttatt taacaaggtc ttttctcgaa
     3661 tattggcata tcaatttaac tttttaaata gtcatcaaaa agataaaaca ccacaatcaa
     3721 caaatttaac gaggaagaat aaaaaataaa tcaacatatt aaattgtagt gttattcaac
     3781 tccgtagcta acaattctct atgcacatta aacaaattgt caaaaatata tcataaatct
     3841 tcaagcacag acttagcgca tcaatcactg aactgttata atagtttggg attaaaggag
     3901 gccgaaacaa tgcaaaaagt tgaaagtatc ataattggtg gagggccatg cggattaagt
     3961 gcggctattg aacaaaaaag aaaaggtatt gataccttaa ttattgaaaa gggtaatgtc
     4021 gttgaatcaa tctacaatta tcctactcac caaacatttt tctcatcaag tgataaatta
     4081 agtattgggg acgtaccgtt tatcgttgaa gaaagtaaac caagacgtaa tcaagcgcta
     4141 gtttattacc gagaagttgt aaaacatcat caattaaaag taaatgcatt tgaagaagta
     4201 ttaactgtta aaaaaatgaa taataaattt actattacta cgacgaaaga tgtttatgaa
     4261 tgtcgatttt taacaatcgc gacaggctat tatggtcagc ataatacatt agaagttgaa
     4321 ggtgcggatt tacctaaagt gttccattat tttaaagagg cacatccgta ttttgatcaa
     4381 gatgttgtaa ttatcggtgg taagaattcg gctatcgatg ctgctttgga gttggaaaaa
     4441 gctggtgcta acgtgacggt tctatatcgt ggtggagatt attcgccttc aattaaaccg
     4501 tggatacttc caaatttcac agcattagta aatcatgaaa aaattgacat ggaatttaat
     4561 gctaatgtta cccaaataac tgaagatact gtgacttatg aagtaaatgg tgaaagtaaa
     4621 acgatacaca atgattatgt atttgcgatg attggttatc atcccgatta tgaattttta
     4681 aaatctgtag gcattcaaat taatacaaat gaatttggaa cagcgcctat gtataataaa
     4741 gaaacatacg aaacaaatat cgaaaattgc tatattgcag gtgtaattgc tgcagggaac
     4801 gatgcgaata ccatttttat tgaaaatggt aaattccacg ggggcattat tgctcaaagc
     4861 atgctagcta agaaacaaac gcccttagaa tcataaaaat aaaggtctat gtactaaata
     4921 acttagtttt acaacgactg acattcatga tatgtcaaat gaggttgatg actaatgatt
     4981 gtacatagac ctttttatgt tacgtattca ttataattca aaatatgatt tgatttcagc
     5041 tttatctaaa ttgttgctta acgcgactaa taattttaat cttgcttttg gaccattcaa
     5101 gccgttagaa aaaataaaac cttgttgtgc gagttggtaa ccaccaccat cgtatgcgta
     5161 agttggactc acaataccat taaaggaacg tgaaactagc acaataggta tatttaaaga
     5221 tactaattgt tgaatgcctt ctaatgcgct tggaggtatg ttgccttgtc ctaacgcttc
     5281 aataaccata ccatcgatac cttctcgact ataaaaacta aaaatgtcac ctggcatacc
     5341 catatatgct tttactaatg gtacatttag tttgtcattg acattttcca atgcttgttg
     5401 gcgatatggc atatgatgga attgcacacg atcctttgtc aatacaccta gcggaccatg
     5461 atttggactt tgaaatgtgt ttgtattaga cgtatgtgtt ttggtaacat tacgcgccgt
     5521 atgaatttca tcattaaata caaccatcac gcctttatga cgggcctttt catcagaggc
     5581 aacgcgaata gcggaaatat aattatataa tccgtcagaa ccaatttcat tagacgagcg
     5641 cattgcgcca gtaataacaa caggttgctc gatacctaat attaaatcaa gtaaaaaggc
     5701 agtttcttct aacgtatcgg taccatgcgt gataacgaaa ccatcataat atttatttgt
     5761 tactgcttct aaaataatat cctttaattg tttaacatgt tggattgtca tatgaggtga
     5821 tggtacatta aaaggattta attcatctat ttgtgcatat tgatttatga catcttgatg
     5881 cattgaaata gggttgatat catttgttac tactttatta gattggtctt gtgacatact
     5941 aatggtgcca ccagtatgaa taacaagtag atgtttcata tatttcctcc tatatttaat
     6001 ttacctaatt atgataaaat attattcata aaacgacaag gaagggaaat gacgcatgaa
     6061 agccattaat attgcattag atggtccagc tgctgccgga aaaagtacaa ttgcgaaacg
     6121 tgtagccagc gaactatcaa tgatttatgt cgatacagga gcaatgtatc gtgcattaac
     6181 atacaaatat ttaaaattaa acaaaactga ggactttgca aaactagttg accaaacaac
     6241 attagattta acttataaag cagataaagg tcaatgtgtc attttagata acgaagatgt
     6301 aacagacttt ttaagaaata atgatgtgac gcaacatgtt tcatacgttg catctaaaga
     6361 gccagtacgt tcattcgccg ttaaaaaaca aaaagagtta gctgcagaaa aaggtatcgt
     6421 aatggatggt cgcgatatcg gaactgtagt gctaccagat gcagatttaa aagtatatat
     6481 gattgcatca gttgaagagc gagcagaaag aagatataaa gataatcaat taagaggtat
     6541 cgaatcaaat tttgaagatt taaaacgtga tattgaagct cgtgatcaat atgacatgaa
     6601 ccgtgaaata tcaccattaa gaaaagcaga tgatgcagtg acattagata cgacaggcaa
     6661 gtcgattgaa gaagttactg acgaaatttt agcgatggtg agtcaaatta aataatctaa
     6721 taagtcataa ctatctattt gtaatgtgca acgcttaacg catatacaaa atgaatgtgc
     6781 tgataatgat ttactcaaat taaaaggtga tttttattca atgatgaatg aaagttgcct
     6841 ttttattttt ggtaaaagtt aatgcgtcag tgaattgtgt aagtttttca aaaagtaaaa
     6901 agaaataata aaggtgaatt attagaattc caaaaataat tcattacatt cataaagcat
     6961 tttacaaatg gtaagaaaat gagtgttaca aatctaaata ttgcaaaaga agctgattta
     7021 gtcacaaaa
//
LOCUS       DAKTIO010000139         7024 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.139, whole genome shotgun sequence.
ACCESSION   DAKTIO010000139 DAKTIO010000000
VERSION     DAKTIO010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7024
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.139"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(23..256)
                     /gene="nrdH"
                     /locus_tag="O0293_002147"
     CDS             complement(23..256)
                     /gene="nrdH"
                     /locus_tag="O0293_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCY0281019.1"
                     /translation="MSEIIVYTQNDCPPCTFVKNYLNEHHIDFEERNINNQQYRNEMI
                     DFDAFSTPFILLNGNPMYHVDLDEINKVLNIQD"
     gene            454..1815
                     /locus_tag="O0293_002148"
     CDS             454..1815
                     /locus_tag="O0293_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCY0281020.1"
                     /translation="MDTVEISRFLTAMTLAVHIIFATIGVGMPLMFAIAEFLGIRKND
                     LQYIAMAKRWAKAYTITVAVGVVTGTIIGLQLSLIWPTFMEMGGHVIALPLFMETFAF
                     FFEAIFLSIYLYTWDRFKNKWTHFLISIPVIIGGSFSAFFITSVNSFMNTPAGFELKN
                     GKMVNVQPIEAMFNPSFIVRSFHVITTAGMTMAFVIASIAAFKLLRNRQPKDTVYHKK
                     ALKMSMIVGFFSTLLSMLAGDLSAKFLHKFQPEKLAAYEWHFDTSSHAKLLLFGVLDE
                     KTQQVKGAIELPGLLSFLADNSVKTKVQGLNDFPKSLHPPMIVHYFFDLMVTMGILCF
                     VISGVYVLTLMFKKLRKFSTHKWMLYGILLTGPASMLAIEFGWFLTEMGRQPWIVRGY
                     MRVAEAATQAGGITFVTILFGILYIILMYTCAYVLIRMFKNKPAYEDVNRLAKKQGGE
                     IEK"
     gene            1812..2831
                     /locus_tag="O0293_002149"
     CDS             1812..2831
                     /locus_tag="O0293_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCY0281021.1"
                     /translation="MIYAFIGITVLWLFLFCYIIIASIDFGAGFFALHSKLTGDEKKI
                     NHLISRYLNPVWEVTNVFFVFFFVGFVGFFPESIKYLGTVLLIPGSIALIMISLRNSF
                     YAFENYGQDTKLAWMIMYGVSGLLIPASLSTALTITEGGYINVRNNVIDLDWVQLLLS
                     PFAWSVVFLAIISVLYISSGFLTYYAKKANDEPAYNLTRQWHIFLGPPMIIICLFVFL
                     SLRIQNSEHFYSAVFDYWWMFGISFLFFALASLLTFFKKKHGLAFVFVILQMMFAFFG
                     YGISKLPYLLYPFVKITDAYVNPEMGWTLVIVFILGLLLLLPSLILLLRLFVFDKEYV
                     EGKKS"
     gene            2964..3626
                     /locus_tag="O0293_002150"
     CDS             2964..3626
                     /locus_tag="O0293_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HCY0281022.1"
                     /translation="MGKEYVVIGLGRFGGSIVRELNALDMDVMAIDHDENRVNEYSDI
                     ATHAVVADTTDEAVMKSLGIRNFDHVIVAIGENIQSSTLTTLILKELGVKKVTAKAQN
                     DYHAKILNKIGADTVVHPERDMGRRIAHNVASASVLDYLELADEHSIVELKATEKMAG
                     QSIIDLDIRAQYGINIIAIKRGKEFIISPNPNINLEIGDILIMIGHDNDLNRFEKNIA
                     TR"
     gene            complement(3975..5672)
                     /locus_tag="O0293_002151"
     CDS             complement(3975..5672)
                     /locus_tag="O0293_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCY0281023.1"
                     /translation="MKQLHPNEVGVYALGGLGEIGKNTYAVEYKDEIVIIDAGIKFPD
                     DNLLGIDYVIPDYTYLVQNQDKIVGLFITHGHEDHIGGVPFLLKQLNIPIYGGPLALG
                     LIRNKLEEHHLLRTAKLNEINEDSVIKSKHFTISFYLTTHSIPETYGVIVDTPEGKVV
                     HTGDFKFDFTPVGKPANIAKMAQLGEEGVLCLLSDSTNSLVPDFTLSEREVGQNVDKI
                     FRNCKGRIIFATFASNIYRVQQAVEAAIKNNRKIVTFGRSMENNIKIGMELGYIKAPP
                     ETFIEPNKINTVPKHELLILCTGSQGEPMAALSRIANGTHKQIKIIPEDTVVFSSSPI
                     PGNTKSINRTINSLYKAGADVIHSKISNIHTSGHGSQGDQQLMLRLIKPKYFLPIHGE
                     YRMLKAHGETGVECGVEEDNVFIFDIGDVLALTHDSARKAGRIPSGNVLVDGSGIGDI
                     GNVVIRDRKLLSEEGLVIVVVSIDFNTNKLLSGPDIISRGFVYMRESGQLIYDAQRKI
                     KTDVISKLNQNKDIQWHQIKSSIIETLQPYLFEKTARKPMILPVIMKVNEQKESNNK"
     gene            complement(5672..5890)
                     /locus_tag="O0293_002152"
     CDS             complement(5672..5890)
                     /locus_tag="O0293_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HCY0281024.1"
                     /translation="MAVFKVFYQHNRDEVIVRENTQSLYVEAQTEEQVRRYLKDRNFN
                     IEFITKLEGAHLDYEKENSEHFNVEIAK"
     gene            complement(5919..6041)
                     /locus_tag="O0293_002153"
     CDS             complement(5919..6041)
                     /locus_tag="O0293_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281025.1"
                     /translation="MNDMMNLVINQIVIKLHYRDDWNEFIDIYSNTILLGLTIN"
     gene            complement(6371..6922)
                     /locus_tag="O0293_002154"
     CDS             complement(6371..6922)
                     /locus_tag="O0293_002154"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCY0281026.1"
                     /translation="MLTMKDIIRDGHPTLRQKAAELELPLTKEEKETLIAMREFLVNS
                     QDEEIAKRYGLRSGVGLAAPQINISKRMIAVLIPDDGSGKSYDYMLVNPKIVSHSVQE
                     AYLPTGEGCLSVDDNVAGLVHRHNRITIKAKDIEGNDIQLRLKGYPAIVFQHEIDHLN
                     GVMFYDHIDKDHPLQPHTDAVEV"
BASE COUNT         2236 a         1061 c         1213 g         2514 t
ORIGIN      
        1 atgaagttga tttagatgaa aattaatctt ggatatttaa tactttgttg atttcatcaa
       61 gatcaacatg gtacattgga ttgccattca acaaaataaa cggagttgaa aaagcatcaa
      121 aatctatcat ttcgtttcga tattgttgat tgttgatatt tctctcttca aaatcaatgt
      181 gatgctcatt tagataattt tttacaaatg tacaaggtgg acaatcattc tgcgtataaa
      241 cgattatttc tgacatgtat catgctcctt cgtgctttat tctaatgtaa tatacacaat
      301 tatacattga aattcaaatt tgtgaacaca ttgtgaactg acataaatta tacacaattt
      361 catagcgcat aatgttttaa aaatacacat tatagcaatt ataattaagt tcattgtgtt
      421 gtcatcaaaa aaagaaaaag gtgatgtttt taaatggata cagttgaaat cagtcggttt
      481 ttgacagcta tgactttagc agttcatatc atttttgcaa cgattggtgt tggtatgcct
      541 ttaatgttcg caattgcaga atttttaggt attcgcaaaa atgatcttca atatatagct
      601 atggccaaaa gatgggctaa agcttataca attactgtag cagtgggagt tgttacaggt
      661 acaattatag gacttcaatt atcattgatt tggcctacat ttatggaaat gggtggacac
      721 gttattgcac ttccattatt tatggaaaca tttgcgttct tctttgaagc tattttctta
      781 agtatatatt tatatacttg ggatcgtttt aaaaataaat ggacacattt cttaattagt
      841 ataccagtaa ttattggtgg ctctttctca gcattcttca ttacttcagt gaattcattt
      901 atgaatacgc ctgcaggttt tgagttgaag aatggaaaga tggtcaatgt tcaacctata
      961 gaagcgatgt ttaacccatc gtttatagtt cgatcatttc acgtaattac tacagccggt
     1021 atgacgatgg catttgttat tgcatcaata gcagctttta aattattgcg taatcgtcaa
     1081 cctaaagata ctgtctacca taagaaagct ttgaaaatgt ctatgatagt tggattcttt
     1141 tcaacattac tttctatgtt ggcaggggat ttatctgcaa aatttttgca taaattccaa
     1201 cctgaaaaat tagcagctta tgaatggcat ttcgatacat cttcccatgc caaattatta
     1261 ttatttggtg tgttagatga aaagactcag caagttaaag gtgcgattga attacctgga
     1321 ctattgagtt tcttagcaga taatagtgtc aaaactaaag tacaagggtt aaatgatttt
     1381 ccaaaaagtt tacatccgcc tatgatcgtc cattatttct ttgatttaat ggtaacgatg
     1441 ggaattttat gttttgtcat ttcaggtgtt tatgtcttaa ctttaatgtt taaaaagctt
     1501 agaaagtttt ctactcataa atggatgctt tatggaatat tattaacagg cccagcttca
     1561 atgctagcta tagaatttgg atggttctta acagagatgg gtagacagcc ttggattgtt
     1621 cgtggttata tgcgcgtggc agaagcagca acacaagcag gcggaataac cttcgttaca
     1681 attttatttg gcatattgta catcatttta atgtatacat gtgcatacgt attaattcgt
     1741 atgtttaaaa ataaaccggc gtatgaagat gtaaatcgtt tagccaagaa gcaaggagga
     1801 gaaatagaaa aatgatttat gcatttatag gtataacagt gttatggttg tttttatttt
     1861 gctatatcat tattgcttct atagattttg gggcaggttt cttcgcattg cattcaaagt
     1921 taactggtga tgaaaagaaa attaatcact taatttcacg ttatttaaac ccagtttggg
     1981 aagttacgaa tgtattcttt gttttcttct tcgtaggatt cgtaggtttc tttccagagt
     2041 caatcaaata tttaggcacg gtattgttaa taccaggttc aatagcactg attatgatat
     2101 cgttgagaaa cagtttttat gcatttgaaa attatggtca agatacaaaa ttagcatgga
     2161 tgatcatgta tggggtaagt ggattattaa ttccagcttc attatctact gctttaacta
     2221 ttacagaagg tggctatatt aatgttcgaa acaatgttat cgatctagat tgggtgcagt
     2281 tattattaag cccatttgct tggtctgtag tattcttggc aattatttca gttttatata
     2341 tttcatcagg atttttgaca tattatgcta aaaaagcaaa tgacgaacca gcatataatt
     2401 taacaagaca atggcacata tttttagggc cgccgatgat tatcatttgt ttattcgtat
     2461 ttctatcatt acgcattcaa aattctgaac atttttattc agctgttttt gactattggt
     2521 ggatgtttgg tataagtttc ttattctttg cattagcttc attgttaaca ttctttaaga
     2581 aaaaacatgg tttggctttt gtatttgtta ttttacaaat gatgttcgcg ttctttggct
     2641 atggaattag taaattgcca taccttttat atccgtttgt aaaaattaca gatgcatatg
     2701 ttaatccaga aatgggctgg acattagtga ttgtctttat tttaggttta cttttattac
     2761 ttccatcgtt aatattatta ttaagattat ttgttttcga caaagaatat gttgaaggaa
     2821 agaaatcata agttgagacg ataccccaac tttcatttac ttgcattact gcatttagat
     2881 aatttagttg gggtactcat ttataataat tttaaattta tattttcaat gaattgatga
     2941 aaatcattta atggagggcg agtatgggta aagaatatgt agtcatcggt ctaggtcgtt
     3001 ttggaggtag tatcgttcgt gagttgaatg cattagacat ggatgtaatg gccatcgacc
     3061 atgatgaaaa tagagtgaat gaatatagtg atatcgctac tcatgcggtt gttgcagaca
     3121 ctacagatga agcagttatg aaaagtttag gtatccgtaa ctttgatcat gtcattgtgg
     3181 caattggtga aaatattcaa tcaagtacgt tgacgacttt aattttaaaa gagttaggtg
     3241 taaaaaaagt aactgctaaa gcacaaaatg attatcatgc aaagatttta aataaaattg
     3301 gagcagatac ggttgtgcac cctgagcgtg atatgggtag acgtattgcg cataatgttg
     3361 cgagtgcaag tgtacttgat tatcttgagt tggcagacga gcattctatt gtagaattga
     3421 aagcaactga aaagatggcg gggcagtcta tcattgattt agatataaga gcacaatatg
     3481 gaattaacat tattgcaatt aaacgaggca aagagttcat catttcacca aatccaaata
     3541 ttaatttaga aataggtgat attttaatca tgattggaca tgataatgat ttaaatcgct
     3601 ttgaaaaaaa tattgcgacg agataatcgg gagtgggaca gaaatgatat tttctcaaaa
     3661 tttatttcgt cgtcccaccc caacttacat tgtctgtaga aattgggaat ccaatttctc
     3721 tttgttgggg cccacacccc aactcgcatt gcctgtagaa tttcttttcg aaattctcta
     3781 tgttggggcc cctgactaga attgaaaaaa gcttgttaca agcgcatttt cgttcagtca
     3841 actactgcca atataacttt gtagagcatt gaacattgat ttatgtctca agctcatcat
     3901 atataaagaa aaagctacta acaatgggtg gtgagtttaa ttaaaacttc acttcaaagt
     3961 tagtagcttt ttgattattt attgtttgat tctttttgtt cgtttacctt cataatgact
     4021 ggtaaaatca ttggttttct agctgttttt tcaaataaat aaggttgtaa tgtttcaatg
     4081 atagaagatt taatctgatg ccattgaata tctttatttt gatttaactt actaataaca
     4141 tcagttttga ttttgcgttg tgcatcataa attaattgac ctgattccct catatataca
     4201 aatcctcgag aaataatgtc tggaccagaa agtaatttat ttgtattaaa atcaatacta
     4261 acaacaacga taactaaacc ttcttcagat aatagcttac ggtctcttat tacaacatta
     4321 ccgatatcac cgataccact accatcaaca agtacattac cagatggaat gcgaccagct
     4381 ttacgtgctg aatcgtgtgt taaagctaag acatctccaa tatcaaagat gaagacatta
     4441 tcttcttcaa cgccgcattc aacaccagtc tcaccatgtg cttttaacat acggtattca
     4501 ccatgaatag gtaagaaata tttcggcttg attaatcgaa gcattaattg ttgatcacct
     4561 tgagaaccat gccctgaagt atggatgtta gaaatcttgc tatggataac atctgcacca
     4621 gctttataca aggaattaat agttctgtta atactttttg tattacctgg gataggtgat
     4681 gaactaaata caacggtatc ttcaggtata attttaattt gcttatgagt accattagca
     4741 attctagata atgctgccat tggttcacct tgtgaaccag tacatagtat caataactca
     4801 tgcttcggta cggtattaat tttattaggt tcaataaatg tttcaggtgg tgctttaata
     4861 taaccaagtt ccatacctat tttaatattg ttttccatcg aacgaccgaa cgtaacaatt
     4921 ttacggttat ttttgatagc agcttcaact gcttgttgaa ctcggtaaat attagaagcg
     4981 aaggtagcaa atataatacg acctttacaa ttacggaaga tcttatctac gttttgacca
     5041 acttcacgtt cgcttaaagt aaaatcaggc acaagtgaat ttgttgagtc tgaaagtaaa
     5101 catagaacgc cttcttcgcc taattgagcc attttagcaa tgtttgctgg tttgcctaca
     5161 ggtgtaaaat caaatttaaa gtcaccggta tgaactactt ttccttcagg tgtatctacg
     5221 atgacgccat aagtttcagg aatactatgt gtagttaagt agaaagaaat cgtaaagtgc
     5281 ttagatttaa tcacactgtc ctcattgatt tcatttagtt tagcagtacg taataaatga
     5341 tgttcttcaa gtttattacg gattaaacct aatgctaaag gaccaccata aataggtata
     5401 ttaagttgtt ttaataggaa gggcacaccg cctatatggt cttcgtgacc atgtgttata
     5461 aataggccaa caattttatc ttggttttga actagatatg tgtagtcagg tataacataa
     5521 tcaatcccta ataagttatc atcagggaat ttgatacctg catcgataat gacaatttcg
     5581 tctttatact caactgcata agtattttta ccgatttcac ctagacctcc aagtgcatat
     5641 acacctactt catttggatg taattgtttc attatttagc aatctccaca ttaaagtgtt
     5701 ctgagttttc tttttcgtaa tctaaatgtg cgccctctaa tttagtgata aattcgatat
     5761 taaaattacg atctttcaag taacgacgta cttgttcttc tgtttgagct tcaacataaa
     5821 gtgattgtgt attttcacgc acaattacct cgtctctgtt atgttgataa aaaactttaa
     5881 atactgccat gttaaaaatt cctcctaaga atgtttgttt aatttattgt taaccctagt
     5941 aaaatcgtat tggagtatat atcgataaat tcattccaat catctctata atgtaactta
     6001 ataacgattt ggttaataac taggttcatc atgtcgttca ttttaaaaaa ttagtgaaat
     6061 aacactaaaa tttcagttaa atcttatttt acatgatgaa tgataataaa taaagcagtt
     6121 tatctcacta gaaatctaag tatttcgaat aaatgtgcag ttctatttat taaaataaat
     6181 gtagagatta taaattaatg ttaagaaaga attaaaggat tttatatgaa acggtaaaat
     6241 ttaaatgagg ggataaaaag tttggtagaa aggtgaatat atttaactgt tgtttattcg
     6301 aaaaagttgt aaaaggaaaa gcttaggaca tcatatttat cctaagcttt gataattaga
     6361 aaaatgtgtt ttaaacttct actgcatctg tatgtggttg taatgggtga tctttgtcaa
     6421 tgtgatcata gaacattaca ccatttaaat ggtcaatttc atgttggaaa acaattgctg
     6481 gatatccttt tagtcgtaat tgtatatcat taccttcgat gtctttggct ttaattgtaa
     6541 ttctattatg acggtgaact agaccagcaa cattatcatc gacactaagg caaccttcac
     6601 cagttggtaa ataagcttct tgaacgctat gacttacaat ttttgggttc acaagcatat
     6661 agtcataaga tttgccactg ccatcatctg gtattaaaac agcaatcata cgtttagaaa
     6721 tattaatttg aggtgcagcc aaaccaacgc ctgaacgtaa accatatcgt ttcgcgattt
     6781 cctcatcttg actatttact aaaaactctc tcatggcgat taatgtttct ttttcttctt
     6841 tagttaatgg taattctaac tcagctgctt tttgacgcaa agttggatga ccatctctaa
     6901 tgatgtcttt cattgttaac atatattgca ccttccttat tttaatttgt tttagttgaa
     6961 tgacagtaaa aaggttgtta agatactcat acatttttat gtgtaaatat ctacaaagtt
     7021 aacc
//
LOCUS       DAKTIO010000140         6928 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.140, whole genome shotgun sequence.
ACCESSION   DAKTIO010000140 DAKTIO010000000
VERSION     DAKTIO010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6928
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.140"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(146..574)
                     /locus_tag="O0293_002155"
                     /pseudo
     CDS             complement(146..574)
                     /locus_tag="O0293_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(769..993)
                     /locus_tag="O0293_002156"
     CDS             complement(769..993)
                     /locus_tag="O0293_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HCY0281027.1"
                     /translation="MTKTLELSFKSSLDKPVKLQLPQLNNVVTEQVARDSMNALVDLN
                     IIKSNSGTITKVHSAQIIDKTTTVLFEDKK"
     gene            complement(1010..1213)
                     /locus_tag="O0293_002157"
     CDS             complement(1010..1213)
                     /locus_tag="O0293_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HCY0281028.1"
                     /translation="MSKINHITIVLSFTKVDANGKQTEFKRRFANINPDASNDQIKTF
                     SKLIERLTGETYSNIELIKSLSI"
     gene            1372..1932
                     /locus_tag="O0293_002158"
     CDS             1372..1932
                     /locus_tag="O0293_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCY0281029.1"
                     /translation="MNKVEAIKFNDDIVKMYEALKIKSERDYLFFKLAIHSGLKVSEL
                     LTITVSQVKRLIEKCTLSEMCKAHFHSLIKIRLPETLSKELLQYIEDRSLSNEDVLFQ
                     SLRTNQVLSRQQAYRIIHQASIEAGIDNVGLTTLRKTFAYHAYQKGIPIPVIQKYLGH
                     QSAIETLNFIGLENECEHSIYISLQL"
     gene            1951..4353
                     /gene="mnhA2"
                     /locus_tag="O0293_002159"
     CDS             1951..4353
                     /gene="mnhA2"
                     /locus_tag="O0293_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit A"
                     /protein_id="HCY0281030.1"
                     /translation="MSLVYLLIAILVIMAMILLMSKRRALAKYAGYIALVAPVISSIY
                     FLIQIPSVAKLQYLSTSIPWIKTLDINLDLRLDGLSLMFSLIISLIGIAVFFYATQYL
                     SSRKDNLPRFYFYLTLFMFSMIGIVLSDNTILMYIFWELTSVSSFLLISYWYNNGDSQ
                     FGAIQSFMITVFGGLALLVGFIMLYIMTGTNNITEILGQADHIKNHGLFIPMIFMFLL
                     GAFTKSAQFPFHIWLPRAMAAPTPVSAYLHSATMVKAGIFLLLRFTPLLGLSNMYVYI
                     VTFVGLITMLFGSITALKQWDLKGILAYSTISQLGMIMAMVGIGGGYAQHQQDAIASI
                     YVFVLFGALFHLMNHAIFKCALFMGVGILDHEAGSRDIRILSGMRQLFPKMNLVMTIA
                     ALSMAGVPFLNGFLSKEMFLDALTQTGQLSQFSLISMIAIVFVGVIASVFTFTYALYM
                     VKEVFWTKYDSKVFTKKNIHEPWLFSLPSLILMVLVPVIFFVPNIFGKGIIVLALRAV
                     SGGNHQIDQLAPHVSQWHGFNIPLLLTIIIILLGSVLAIKVDWKKVFTGKIRQISVSK
                     SYEMVYRHFEKFATKRFKRVMQDRLNQYIIMTLGIFMIIIGYGYIRIGLPKVHQLHVS
                     EFGALEIILAIVTVTIGISLIFIRQRLTMVILNGVIGFVVTLFFIAMKAPDLALTQLV
                     VETITTILFIVSFSRLPNVPRSNANKKREIIKISVSLLMALIVVSLIFITQQTDGLSS
                     ISDFYLKADKLTGGKNIVNAILGDFRALDTLFEGLVLIITGLGIYTLLNYQDRRGQDE
                     RE"
     gene            4340..4765
                     /gene="mnhB2"
                     /locus_tag="O0293_002160"
     CDS             4340..4765
                     /gene="mnhB2"
                     /locus_tag="O0293_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit B"
                     /protein_id="HCY0281031.1"
                     /translation="MKENDVVLRTVTKLVVFILLTFGFYVFFAGHNNPGGGFIGGLIF
                     SSAFILMFLAFNVEEVLESLPIDFRILMIIGALVSSITAIIPMFFGKPFLSQYETTWI
                     LPILGQIHVSTITLFELGILFSVVGVIVTVMLSLSGGRS"
     gene            4762..5106
                     /gene="mnhC2"
                     /locus_tag="O0293_002161"
     CDS             4762..5106
                     /gene="mnhC2"
                     /locus_tag="O0293_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit C"
                     /protein_id="HCY0281032.1"
                     /translation="MNLILLLVIGFLVFIGTYMILSINLIRIVIGISIYTHAGNLIIM
                     SMGTYGSSRSEPLITGGNQLFVDPLLQAIVLTAIVIGFGMTAFLLVLVYRTYKVTKED
                     EIEGLRGEDDAK"
     gene            5096..6592
                     /gene="mnhD2"
                     /locus_tag="O0293_002162"
     CDS             5096..6592
                     /gene="mnhD2"
                     /locus_tag="O0293_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit D"
                     /protein_id="HCY0281033.1"
                     /translation="MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTII
                     SLMLLIYVQRHRPITLDFGGWSAPFGIQFLGDSLSLIMVTTASFVITLIMAYGFGRGE
                     HKANRYHLPSFILFLSVGVIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAA
                     IIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFS
                     AKAALVLFMWLPKAYAVLNTELAALFAALMTKVGAYALIRFFTLLFDQHNDLIHPLLA
                     TMAAITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTFAGINGAIFYLVNDI
                     VVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPLFGVAFIIMIFAIGGVPPFSGFPGK
                     VLIFQGALQNGNYIGLALMIITSLIAMYSLFRILFYMYFGDKDGEEVNFKKIPLYRKR
                     ILSILVVVVIAIGIAAPVVLNVTSDATELNTSDQLYQKLVNPHLKGED"
     gene            6593..>6928
                     /gene="mnhE2"
                     /locus_tag="O0293_002163"
     CDS             6593..>6928
                     /gene="mnhE2"
                     /locus_tag="O0293_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit E"
                     /protein_id="HCY0281034.1"
                     /translation="MNQIVLNIIIAFLWVLFQDEDHFKFSTFFSGYLIGLIVIYILHR
                     FFSDDFYVRKIWVAIKFLGVYLYQLITSSISTINYILFKTKDMNPGLLSYETRLTSDW
                     SITFLTILII"
BASE COUNT         2256 a          965 c         1181 g         2526 t
ORIGIN      
        1 acttctaata tacgaaaata tgcaaaatta tacattttac aaatttttct tttagaaaca
       61 ccatgaaatc ttaaaattac aaatttaaga taatttaatt gatttgaacc atcaaaaaaa
      121 ggtgtaaact cagtcataat tatcaattcc tttcataaga attaactgtt aactaaagtt
      181 tacaccacca attaaaatca tgaattgtag cgccaaataa tccaaatgta tcaataatga
      241 cacccataat aatttgaccc gcaactgttg caattacagt taatgttgca cctaattttg
      301 gcaataacaa taaattgcca gttaaaaagc taaccccaag caaaccaccg actacccatg
      361 tgtagttaaa tgattgatta ttgtaaaagt gaatagtaaa tacttctgga ttgataatga
      421 tatttaaaat aattaaacaa attgttccaa ctgaaaatga aatgaatgaa gtatagaaag
      481 gcgatttagt atacagtgat agtcttgaat tgacagatgt ttggatagga ataagcattc
      541 caactaggac accaatgata tagaaaagaa gcatatgaat aaagtctcac tttccaaagt
      601 taataataaa atcatatatt tacattatca ttatagagaa aatatacaac tcatttacaa
      661 taatgttgaa aaaaacagga cattattaca acaaaatcac cctagacaat catgatgaaa
      721 gtctagggtg ttagtattta tactaaatta attgcgcttt ctttatatct atttcttatc
      781 ttcaaataaa acagttgttg ttttatcaat aatttgagca gaatgtactt tggtgatagt
      841 accactattt gactttataa tatttaaatc tactaatgca ttcatactat cacgtgcaac
      901 ttgctcggtc actacgttgt ttaattgtgg taattgcagt tttacaggct tgtctagtga
      961 agatttaaaa cttaattcaa gtgttttagt catgaatatg tcctcctgat taaattgata
     1021 aagatttgat gagttcgata ttgctatatg tttctccagt aagtcgctca ataagtttgc
     1081 tgaatgtttt aatttggtcg tttgatgcat cagggttaat gttagcgaat cgacgcttaa
     1141 attctgtttg tttgccgtta gcgtctactt tagtaaatga taatacaata gtgatgtggt
     1201 ttattttact catattttaa aacctccttt cacactatat atcgaaacaa aataataaaa
     1261 tggctaattt tattttctat gtttaaaatc tataaaaaag gcaatagata tatgtaacta
     1321 aaatataata tagctatgat taagacactt aaagcaaggg gaggttgagt aatgaataaa
     1381 gtagaagcga ttaaatttaa tgatgatatt gttaaaatgt atgaagcgct caagataaaa
     1441 tctgaacgtg actatttatt ctttaagtta gctatacata gtggattgaa agtatcagaa
     1501 ttattaacaa ttacagtctc tcaagttaag agactaattg aaaagtgtac gttatcagaa
     1561 atgtgtaaag cacattttca ttcgttgatt aaaattaggt taccagaaac attatcgaaa
     1621 gaactacttc aatatataga ggacaggagt ctttcgaatg aagacgttct ttttcaatca
     1681 ctacgaacaa atcaagtatt atctagacag caagcatatc gaataattca ccaagcatca
     1741 attgaagctg gtatagataa tgtaggacta acgacattgc gtaagacatt tgcatatcat
     1801 gcttatcaaa aaggtatacc tataccagtc attcaaaagt atttagggca tcaatctgct
     1861 attgaaacac taaattttat cggtttagaa aatgagtgtg aacatagtat ttatatttca
     1921 ttacaattat agaaacaaag gaggctaata atgagtttgg tttatttatt aattgctata
     1981 cttgtgatta tggcgatgat acttctaatg tctaaacgta gagcattggc taaatatgcc
     2041 gggtacatag cgttggttgc acctgtaatt tcatctatct attttttgat tcaaatacca
     2101 tcagtagcta aactgcaata tctttctacc tctattccat ggattaagac attagatatt
     2161 aatttagatt tacgtttaga tggtttaagt ttaatgtttt ctcttattat ttcacttatt
     2221 ggaattgcag tattcttcta tgcaactcaa tatttatcct ctcgaaaaga caatttacca
     2281 aggttttatt tttatttaac gttatttatg ttcagtatga ttggtattgt attatcagac
     2341 aatacgatat tgatgtacat tttttgggaa ttaacgagtg tatcatcatt tttattgatt
     2401 tcatattggt ataacaatgg tgacagtcaa tttggtgcga ttcaatcatt tatgattaca
     2461 gtatttggtg gattggcgtt attagttggt tttattatgc tgtatatcat gacaggaacg
     2521 aataacatca cagagatatt aggacaagca gatcatatta agaatcatgg attgtttatc
     2581 cctatgattt ttatgttttt attaggtgca tttacaaaat cagcacaatt tccatttcat
     2641 atttggctac ctagagcaat ggctgcacct acacctgtaa gtgcttattt acattcagcc
     2701 acgatggtaa aagctggtat ctttttatta cttcgattta caccattatt aggtcttagc
     2761 aatatgtacg tatatatcgt tacgtttgtt ggtttaataa caatgttatt tggttcaatt
     2821 acagctttaa aacaatggga tttaaaaggt atcctagcgt actctacaat cagtcaactt
     2881 gggatgatta tggctatggt gggtataggt ggcggatatg ctcaacacca acaagacgca
     2941 atagcatcta tttatgtatt tgtattattt ggtgcgctat ttcatctaat gaatcatgcc
     3001 atctttaaat gtgcgctttt catgggagta ggtattttag atcatgaagc aggttcaagg
     3061 gatatacgaa ttttaagtgg aatgcgtcaa ctatttccta aaatgaatct agtcatgacg
     3121 atagcggctc tatctatggc tggagtacca tttttaaatg gatttttaag taaagaaatg
     3181 tttttagatg cattaacaca aactggacaa ttatcccaat ttagtttgat ttcaatgata
     3241 gctatcgtgt ttgttggtgt tattgcgagt gtttttacat tcacatatgc actatacatg
     3301 gtaaaagaag tattttggac aaaatatgat tctaaggttt ttactaaaaa aaatatccac
     3361 gaaccatggt tgtttagttt accatctctt atattaatgg tgctagtacc tgtaatcttt
     3421 tttgtaccaa atatatttgg gaaggggatt atcgttctag cattaagagc tgtatcaggt
     3481 ggtaatcatc aaattgatca attggcacca catgtttcgc aatggcatgg atttaacata
     3541 ccgcttcttt taaccatcat cattatttta ttgggtagtg tactagcaat caaagtagat
     3601 tggaaaaaag tgttcacagg taaaattaga cagatttcag tttcaaaaag ctatgagatg
     3661 gtatatcgac attttgaaaa gtttgctacg aagcgattta aacgtgttat gcaagatcgt
     3721 ttaaaccaat acattattat gaccttaggc atatttatga ttatcattgg atatggttat
     3781 attcgaattg gacttcctaa agtacatcag ttacatgttt ctgaatttgg ggcattagaa
     3841 attatattag caatcgtaac tgtcacaatt ggtatttctt taatttttat acgtcaacga
     3901 ctgacaatgg tcattttaaa tggagtcatc ggatttgttg tgaccttatt ctttatagca
     3961 atgaaagccc ctgatctagc attgactcag ctagtagttg aaacaataac gacgatacta
     4021 tttattgtca gtttttcaag attaccaaac gtgccaagat ctaacgctaa caaaaaaaga
     4081 gaaataatta aaatttctgt atcactcttg atggcactta ttgttgtatc attaattttt
     4141 attacacaac aaacagatgg tttatcatca atatcagact tttatttaaa agctgacaaa
     4201 ctaacaggtg gtaaaaatat tgtaaatgcg atacttggtg actttagagc attagataca
     4261 ttatttgaag gattagtgtt aattattact gggctaggta tttacacatt attaaattat
     4321 caagatcgga ggggacaaga tgaaagagaa tgatgtcgtg ttaagaacgg tcacgaaact
     4381 tgttgtattt attttattga ctttcggatt ctatgtcttc ttcgcaggtc ataataatcc
     4441 tggtggtggg tttattggtg gtttaatatt tagttcagcg tttattttaa tgtttctggc
     4501 ttttaatgtt gaagaggttt tagaaagttt accgattgat tttagaattt taatgattat
     4561 tggagcattg gtatcatcta ttactgcgat aatacctatg ttttttggaa aaccattttt
     4621 gtctcaatat gaaacaactt ggatacttcc aattttagga caaattcatg taagtacaat
     4681 aacacttttt gaattaggta ttttattctc agttgttggt gttattgtca cagtgatgtt
     4741 gtcgcttagc ggaggtcgat catgaattta atattattac tagttatagg atttttagtg
     4801 tttataggaa catatatgat tttatcaatc aatttaattc gtattgtaat cggaatttca
     4861 atatatactc atgctggtaa tctcattatt atgagtatgg gaacgtatgg ttctagtaga
     4921 tcagaaccac taataactgg tggaaaccaa ttgtttgttg atcccttgtt acaagctatt
     4981 gtactaactg caatagttat agggtttggg atgactgcgt ttttacttgt acttgtttat
     5041 agaacttata aagtaacaaa agaagatgaa attgaaggcc taagggggga agatgatgct
     5101 aagtaactta ttgattttac caatgttatt accattcctt tgtgccttaa tccttgtatt
     5161 tttaaaaaat aatgatcgta tttctaaata tttatactta ggtacaatga ctatcaccac
     5221 aattatttca ttaatgctat taatttatgt tcagcgtcac cgtccaatta cgctagactt
     5281 tggaggatgg tcagcgccct ttggtataca gtttttagga gattctttaa gtttaattat
     5341 ggttacaacc gcttcgtttg tgattacttt aattatggca tacggatttg ggcgtggcga
     5401 acataaagca aatcgttatc acttgccatc gttcatatta tttttaagtg ttggcgtgat
     5461 aggctctttt ctaacatcag atttatttaa tttatacgtc atgtttgaaa ttatgttact
     5521 agcgtcattt gtactcatta cacttggaca atctgtagaa caattacgtg ctgcaattat
     5581 ttatgttgtc ttgaatatta ttggttcatg gctattctta ttaggtatag gtttacttta
     5641 taaaacagta ggtacattaa acttttcaca tattgcaatg cgtttgaatg acatgggaga
     5701 taatcgcact gttacaatga tttcattaat cttcttagtc gcatttagtg cgaaagcagc
     5761 gctggtcctt tttatgtggc tacccaaagc ctacgctgtg ttaaatactg agcttgcagc
     5821 attatttgca gcgttaatga ccaaagtagg ggcctatgca ttaattcgat tcttcacttt
     5881 actatttgat caacataatg atctcataca tccattgcta gcaactatgg ctgctataac
     5941 tatggtcatc ggcgctatag gtgtcattgc ttataaagat attaaaaaga ttgcagctta
     6001 ccaagtcata atctcaatag gatttatcat tttaggttta ggaacaaaca cgtttgcagg
     6061 tattaatggt gcaatatttt atttggtaaa tgacattgtt gtaaaaacat tgctattttt
     6121 tattattggt agtttagttt acattacagg ctatcgacaa tatcaatatt tgaatggctt
     6181 agctaaaaaa gaacctttat ttggagttgc gtttattata atgatttttg ctattggcgg
     6241 cgtgcctcca tttagtggct ttccggggaa agtacttatt ttccaaggtg cattgcaaaa
     6301 tggcaattat attggactag cgttaatgat tattactagt ctaattgcaa tgtacagttt
     6361 atttaggata cttttttata tgtattttgg agataaagat ggggaggaag ttaattttaa
     6421 gaaaatcccg ctatatcgaa aaagaatttt aagtatttta gtagttgtgg ttatcgcaat
     6481 cggaattgct gcacctgttg tgttaaatgt tacaagtgat gcaactgagt tgaacacgag
     6541 tgatcaatta tatcaaaaac ttgtaaatcc gcatttgaaa ggagaggact aaatgaatca
     6601 aatagtttta aatattatca ttgcattctt atgggtatta tttcaagatg aagatcattt
     6661 taaattctcg actttctttt ctggatatct aattggttta attgtcattt atatattaca
     6721 caggtttttc agcgatgatt tttatgttag aaaaatatgg gtagctatta aatttttagg
     6781 tgtttattta tatcaattaa taacatctag cattagcacg attaattata ttctttttaa
     6841 aacaaaagat atgaaccctg gattactttc atatgaaaca agactaacaa gtgattggtc
     6901 aataacattt ttaacaattt taattatt
//
LOCUS       DAKTIO010000141         6720 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.142, whole genome shotgun sequence.
ACCESSION   DAKTIO010000141 DAKTIO010000000
VERSION     DAKTIO010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6720
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.142"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            151..1380
                     /locus_tag="O0293_002164"
     CDS             151..1380
                     /locus_tag="O0293_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCY0281035.1"
                     /translation="MSLRDEALEMHKRNQGKLEVKPNVKVTNKEELSLAYSPGVAEPC
                     KDIYEDKRKVYDYTIKGNTVAVITDGTAVLGLGNIGPEASIPVMEGKAVLFKSFAGIN
                     GVPIALNTTDTEEIIKTVKLLEPNYGGINLEDISAPRCFEIEERLKKETNIPVFHDDQ
                     HGTAIVTLAGLVNALRVVNKDIAKIKVVLNGAGAAGIAIVKLLYAYGVRNMVMCDSRG
                     AIFEGRSYGMNPTKDVVAKWTNKDKIEGSLEEVVKDADVFIGVSVANALSQDMVKSMA
                     DNPIIFAMANPNPEIIPDDAKAAGARVVGTGRSDYPNQINNVLAFPGIFRGALEVEAT
                     HINEEMKKAAVEAIADLIDSSELNEDYCIPGPFDKRVAPSVARNVAKAAMESGVARIE
                     VDPQDVYDKTMKLTDLQ"
     gene            1575..2432
                     /locus_tag="O0293_002165"
     CDS             1575..2432
                     /locus_tag="O0293_002165"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HCY0281036.1"
                     /translation="MFKDFFNRTKKKKYLTVQDSKNNDVPAGIMTKCPKCKKIMYTKE
                     LAENLNVCFNCDHHIALTAYKRIEAISDEGSFTEFDKGMTSANPLDFPSYLEKIEKDQ
                     QKTGLKEAVVTGTAQLDGMKFGVAVMDSRFRMGSMGSVIGEKICRIIDYCTENRLPFI
                     LFSASGGARMQEGIISLMQMGKTSVSLKRHSDAGLLYISYLTHPTTGGVSASFASVGD
                     INLSEPKALIGFAGRRVIEQTINEKLPDDFQTAEFLLEHGQLDKVVHRNDMRQTLSEI
                     LKIHQEVTK"
     gene            2432..3376
                     /locus_tag="O0293_002166"
     CDS             2432..3376
                     /locus_tag="O0293_002166"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HCY0281037.1"
                     /translation="MLDFEKPLFEIRNKIESLKESQDKNDVDLQEEIDMLEASLERET
                     KKIYTNLKPWDRVQIARLQERPTTLDYIPYIFDSFMELHGDRNFRDDPAMIGGIGFLN
                     GRAVTVIGQQRGKDTKDNIYRNFGMAHPEGYRKALRLMKQAEKFNRPIFTFIDTKGAY
                     PGKAAEERGQSESIATNLIEMASLKVPVIAIVIGEGGSGGALGIGIANKVLMLENSTY
                     SVISPEGAAALLWKDSNLAKIAAETMKITAHDIKQLGIIDDVISEPLGGAHKDIEQQA
                     LAIKSAFVAQLDSLESLSRDEIANDRFEKFRNIGSYIE"
     gene            3645..4613
                     /gene="pfkA"
                     /locus_tag="O0293_002167"
     CDS             3645..4613
                     /gene="pfkA"
                     /locus_tag="O0293_002167"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002462395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCY0281038.1"
                     /translation="MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGL
                     LNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGG
                     DGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTAS
                     SHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHS
                     IVLVAEGCMTAQDCQKELSQYINVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDL
                     LMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI"
     gene            4635..6392
                     /gene="pyk"
                     /locus_tag="O0293_002168"
     CDS             4635..6392
                     /gene="pyk"
                     /locus_tag="O0293_002168"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCY0281039.1"
                     /translation="MRKTKIVCTIGPASESEEMIEKLINAGMNVARLNFSHGSHEEHK
                     GRIDTIRKVAKRLDKIVAILLDTKGPEIRTHNMKDGIIELERGNEVIVSMNEVEGTPE
                     KFSVTYENLINDVQVGSYILLDDGLIELQVKDIDHAKKEVKCDILNSGELKNKKGVNL
                     PGVRVSLPGITEKDAEDIRFGIKENVDFIAASFVRRPSDVLEIREILEEQKANISVFP
                     KIENQEGIDNIAEILEVSDGLMVARGDMGVEIPPEKVPMVQKDLIRQCNKLGKPVITA
                     TQMLDSMQRNPRATRAEASDVANAIYDGTDAVMLSGETAAGLYPEEAVKTMRNIAVSA
                     EAAQDYKKLLSDRTKLVETSLVNAIGISVAHTALNLNVKAIVAATESGSTARTISKYR
                     PHSDIIAVTPSEETARQCSIVWGVQPVVKKGRKSTDALLNNAVATAVETGRVSNGDLI
                     IITAGVPTGETGTTNMMKIHLVGDEIANGQGIGRGSVVGTTLVAETVKDLEGKDLSDK
                     VIVTNSIDETFVPYVEKALGLITEENGITSPSAIVGLEKGIPTVVGVEKAVKNISNNM
                     LVTIDAAQGKIFEGYANVL"
BASE COUNT         2382 a          901 c         1364 g         2073 t
ORIGIN      
        1 ttttattatc gtttatcgct atttgtgtta cataatcaaa accataaatt cttactcatt
       61 cagatttacc caatattttt acttttataa tgtaatgcgt tttatccaag ttatttttta
      121 aaaataaata ttgaattagg ggctgatttc atgtcattaa gagatgaagc attggaaatg
      181 cacaaacgta atcaaggtaa attagaagtt aaaccaaatg taaaagttac taataaagag
      241 gaattaagtt tagcatactc acctggcgtt gctgaaccgt gtaaagatat ttatgaagat
      301 aaaagaaaag tatatgatta cacaattaaa ggaaatacag ttgcagttat tactgatgga
      361 acagcggtat taggtttagg taacattgga cctgaagcaa gtattcctgt aatggaaggt
      421 aaagcagtat tattcaaaag cttcgctggt atcaatgggg tgcctattgc gttaaataca
      481 actgataccg aagaaatcat taaaacagtt aagttgttag aacctaatta tggtggtatt
      541 aatttagagg atatttcggc accacgttgt tttgaaattg aagaacgatt gaaaaaagaa
      601 actaatattc cggtattcca tgacgatcaa catggtacag caattgtaac attggcaggt
      661 ttggtaaatg cattgagagt tgttaacaaa gatattgcta aaataaaagt tgtactaaat
      721 ggtgctggtg cagcaggaat agccattgtt aaattactat acgcgtatgg tgtaagaaat
      781 atggttatgt gtgactcaag aggcgcaatt tttgaaggac gttcatatgg tatgaatcct
      841 acgaaagatg ttgtagcaaa atggacaaat aaagataaga ttgaagggtc tttagaagaa
      901 gtcgtaaaag atgcagatgt atttatcggg gtttctgtag ctaatgcgct gtcacaagat
      961 atggttaaga gtatggcaga taatccaatt atatttgcaa tggctaatcc aaatcctgaa
     1021 ataatacctg atgatgccaa agcggcaggt gcacgagttg ttggtacagg acgttcagac
     1081 tatcctaacc aaattaataa tgtattagct ttccctggta tttttagagg tgcattagag
     1141 gttgaagcta cacatataaa tgaagaaatg aaaaaggcag ctgtagaagc gattgctgat
     1201 ttaatcgata gttctgaatt aaatgaagac tactgtatcc caggaccgtt tgataaacgt
     1261 gtagcgccat cagttgctcg taatgttgct aaagcggcaa tggaatctgg agtagctagg
     1321 attgaagttg atccgcaaga tgtgtatgat aaaacaatga aacttacaga tttacaataa
     1381 aaataattaa cgtttaatta aattgcttaa aagttagcaa taaagagtca ttaaaatgat
     1441 gaaaatgaaa gtttatgata aacattcaac agtcaaacga atataaatca aataaattta
     1501 aacccgtttt taactggtca agttcagttt aaggctctaa atggttagaa cagaggttat
     1561 ttggaggttt tcctatgttt aaagattttt ttaatcgaac aaagaaaaag aaatatctta
     1621 cagtacaaga ctctaaaaat aatgatgtgc ctgcaggtat tatgactaag tgtccaaagt
     1681 gtaagaaaat tatgtacaca aaagaattag ctgaaaattt aaatgtgtgc tttaattgtg
     1741 atcatcatat tgctttaact gcgtataaac gtatagaagc aatttctgat gaaggatcat
     1801 ttacagaatt cgataaggga atgacctctg cgaatccatt agattttcca agttatttag
     1861 aaaaaattga aaaggaccaa caaaagacag gtcttaaaga agcagttgtg actggtacag
     1921 cacaactaga tggtatgaaa tttggcgttg ctgtcatgga ttcacgtttt agaatgggaa
     1981 gtatgggatc ggttatcggt gaaaagatat gtcgcatcat tgattactgc actgagaacc
     2041 gtttaccatt tattcttttc tctgcaagtg gtggtgcacg tatgcaagaa ggtattattt
     2101 ccttgatgca aatgggtaaa accagtgtat ctttaaaacg tcattctgac gctggactat
     2161 tatatatatc atatttaaca catccaacta ctggtggtgt atctgcaagt tttgcatcag
     2221 ttggtgatat aaatttaagt gagccaaaag cgttgatagg ttttgcaggt cgtcgagtta
     2281 ttgaacagac aataaacgaa aaattgccag atgatttcca aactgcagaa tttttattag
     2341 agcatggaca attggataaa gttgtacatc gtaatgatat gcgtcaaaca ttgtctgaaa
     2401 ttctaaaaat ccatcaagag gtgactaaat aatgttagat tttgaaaaac cactttttga
     2461 aattcgaaat aaaattgaat ctttaaaaga atctcaagat aaaaatgatg tggatttaca
     2521 agaagaaatt gacatgcttg aagcgtcatt ggaacgagaa actaaaaaaa tatatacaaa
     2581 tctaaaacca tgggatcgtg tgcaaattgc gcgtttgcaa gaaagaccta cgaccctaga
     2641 ttatattcca tatatctttg attcgtttat ggaactacat ggtgatcgta attttagaga
     2701 tgatccagca atgattggtg gtattggctt tttaaatggt cgtgctgtta cagttattgg
     2761 acaacaacgt ggaaaagata caaaagataa tatttatcga aattttggta tggcgcatcc
     2821 agaaggttat cgaaaagcat tacgtttaat gaaacaagct gaaaaattca atcgtcctat
     2881 ctttacattt atagatacaa aaggtgcata tcctggtaaa gctgctgaag aacgtggaca
     2941 aagtgaatct atcgcaacaa atttgattga gatggcttca ttaaaagtac cagttattgc
     3001 gattgtcatt ggtgaaggtg gcagtggagg tgctctaggt attggtattg ccaataaagt
     3061 attgatgtta gagaatagta cttactctgt tatatctcct gaaggtgcag cggcattatt
     3121 atggaaagac agtaatttgg ctaaaattgc agctgaaaca atgaaaatta ctgcccatga
     3181 tattaagcaa ttaggtatta tagatgatgt catttctgaa ccacttggcg gtgcacataa
     3241 agatattgaa cagcaagctt tagctattaa atcagcgttt gttgcacagt tagattcact
     3301 tgagtcatta tcacgtgatg aaattgctaa tgatcgcttt gaaaaattca gaaatatcgg
     3361 ttcttatata gaataatcaa cttgagcatt tttatgttaa atcgatactg ggttttacca
     3421 taaattgaag tacattaaaa caataattta atatttagat actgaatttt aactaagatt
     3481 agtagtcaaa attgtggcta ctaatctttt tttaattaag ttaaaataaa attcaatatt
     3541 taaaacgttt acatcaattc aatacattag ttttgatgga atgacatatc aatttgtggt
     3601 aatttagagt taaagataaa tcagttatag aaaggtatgt cgtcatgaag aaaattgcag
     3661 ttttaactag tggtggagat tcacctggaa tgaatgctgc cgtaagagca gttgttcgta
     3721 cagcaattta caatgaaatt gaagtttatg gtgtgtatca tggttaccaa ggattgttaa
     3781 atgatgatat tcataaactt gaattaggat cagttgggga tacgattcag cgtggaggta
     3841 cattcttgta ttcagcaaga tgtccagagt ttaaggagca agaagtacgt aaagttgcaa
     3901 tcgaaaactt acgtaaaaga gggattgagg gccttgtagt tattggtggt gacggtagtt
     3961 atcgcggtgc acaacgcatc agtgaggaat gtaaagaaat tcaaactatc ggtattcctg
     4021 gtacgattga caatgatatc aatggtactg attttacaat tggatttgac acagcattaa
     4081 atacgattat tggcttagtc gacaaaatta gagatactgc gtcaagtcac gcacgaacat
     4141 ttatcattga agcaatgggc cgtgattgtg gagatctagc attatgggct ggattatcag
     4201 ttggtgctga gacaattgta gttccagaag tgaaaacaga tattaaagaa atagctgata
     4261 aaattgaaca aggtattaaa cgtggtaaga aacactcaat cgttcttgta gcagaaggtt
     4321 gtatgactgc gcaagattgt caaaaagaat tatcacaata catcaatgtt gataatagag
     4381 tgtctgtgtt aggtcacgtt caacgtggtg gtagcccaac aggtgcggat agagttttag
     4441 catcacgttt aggtggatat gcggtagact tattaatgca aggtgaaaca gctaagggtg
     4501 ttggaattaa gaacaataaa attgtagcaa catcttttga tgaaattttt gatggtaaag
     4561 atcataaatt tgattatagt ctatatgaac ttgctaacaa gttatctata taagatttca
     4621 ggaggaatta taaaatgaga aaaactaaaa ttgtatgtac aattggacca gcttcagaat
     4681 cagaagaaat gattgagaaa ttaatcaatg ctggtatgaa cgttgcacga ttaaactttt
     4741 cacatggtag tcatgaagag cataaaggta gaattgatac aattcgtaaa gtagctaaaa
     4801 gattagacaa aattgtagca attttattag atacaaaagg tccagaaatt cgtacgcata
     4861 atatgaaaga cggtatcatt gaacttgaac gtggcaacga agttattgtt agcatgaatg
     4921 aagttgaagg aacacctgaa aagttctcag taacatatga aaacttaatt aacgatgttc
     4981 aagtaggttc atacatttta cttgatgatg gcttaattga attacaagtt aaagatattg
     5041 accatgctaa aaaagaagtt aaatgtgata ttttaaactc tggtgagctt aaaaacaaaa
     5101 aaggtgttaa cttacctggc gtaagagtaa gtttacctgg tattacagaa aaagatgctg
     5161 aagatatccg tttcggtatt aaagaaaatg ttgacttcat tgcagcaagt ttcgtacgtc
     5221 gtcctagtga tgttttagaa attcgtgaaa ttttagaaga acaaaaagct aacatttcag
     5281 tattccctaa aattgaaaac caagaaggta ttgataatat tgcggaaatt cttgaagtgt
     5341 ctgatggttt aatggttgca cgtggtgaca tgggtgttga aattccacct gaaaaagtac
     5401 caatggttca aaaagattta atcagacaat gtaacaaatt aggtaaacca gttattacag
     5461 ctacacaaat gttagattct atgcaacgta acccacgtgc tacacgtgca gaagctagtg
     5521 acgttgccaa cgcaatctat gatggtacag atgcagtaat gttatctggt gaaactgctg
     5581 ctggtttata tcctgaagaa gctgttaaaa caatgagaaa tattgctgta tcagctgaag
     5641 cagcccaaga ttacaaaaag ttattgtcag atcgtactaa attagttgaa acttcattag
     5701 tgaatgctat cggtatttcg gttgcacata cagctttaaa cttaaatgtt aaagcaattg
     5761 tagctgctac tgaaagtggt tcaacggcac gtactatctc caaatatcgt ccacattcag
     5821 acattattgc ggtgactcca agtgaagaaa ctgcacgtca atgttcaatt gtttggggag
     5881 ttcaacctgt agttaaaaaa ggacgtaaga gtacagatgc attgttaaac aatgcagttg
     5941 caacagctgt tgaaactggt agagtatcta atggtgattt aatcattatt actgctggtg
     6001 taccaactgg tgaaactgga actactaata tgatgaaaat ccacctagtt ggtgacgaaa
     6061 ttgctaatgg tcaaggtatt ggacgtggat cagttgttgg tactacgtta gttgctgaaa
     6121 ctgttaaaga tttagaaggt aaagatttat ctgacaaagt tatcgttact aactcaatcg
     6181 atgaaacgtt tgtaccttat gtagaaaaag ctttaggctt aattacagaa gaaaatggta
     6241 ttacatcacc aagtgcaatt gttggtttag aaaaaggtat tccaacagtt gtaggtgtag
     6301 aaaaagctgt taaaaacata agcaataaca tgttagttac gattgatgct gctcaaggta
     6361 aaatctttga aggatatgca aacgtactat aatttataaa aaaacgtctt tccatttatc
     6421 aacaatggaa aggcgttttt tggttcatct ggtattttat gacgtaatta ataggttatt
     6481 tgataatgat agtgtatgaa tggcaatcta tataaatgtt tatatctttt atacatgtac
     6541 attatcacct tcaaactttc actcatatta ctttggaaat ttattataaa atagaagtat
     6601 ggatgtattt ctgaaatgat acattattaa atagatgaga aagtaaaagt tttgagccaa
     6661 gtacgcaatt taatattata agttgcatat aaaacaggat gggacataaa tccctaaaaa
//
LOCUS       DAKTIO010000142         6697 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.143, whole genome shotgun sequence.
ACCESSION   DAKTIO010000142 DAKTIO010000000
VERSION     DAKTIO010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6697
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.143"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            12..3476
                     /gene="sdrE"
                     /locus_tag="O0293_002169"
     CDS             12..3476
                     /gene="sdrE"
                     /locus_tag="O0293_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrE"
                     /protein_id="HCY0281040.1"
                     /translation="MINRDNKKAITKKGMISNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAENTSTENAKQDDATTSDNKEVVSETENNSTTENNSTNPIKKETNTDSQP
                     EAKKESTSSSTQKQQNNVTATTETKPQNIEKENVKPSTDKTATEDTSVILEEKKAPNN
                     TNNDVTTKPSTSEPSTSEIQTKPTTPQESTNIENSQPQPTPSKVDNQVTDATNPKEPV
                     NVSKEELKNNPEKLKELVRNDSNTDHSTKPVATAPTSVAPKRVNAKMRFAVAQPAAVA
                     SNNVNDLIKVTKQTIKVGDGKDNVAAAHDGKDIEYDTEFTIDNKVKKGDTMTINYDKN
                     VIPSDLTDKNDPIDITDPSGEVIAKGTFDKATKQITYTFTDYVDKYEDIKSRLTLYSY
                     IDKKTVPNETSLNLTFATAGKETSQNVTVDYQDPMVHGDSNIQSIFTKLDEDKQTIEQ
                     QIYVNPLKKSATNTKVDIAGSQVDDYGNIKLGNGSTIIDQNTEIKVYKVNSDQQLPQS
                     NRIYDFSQYEDVTSQFDNKKSFSNNVATLDFGDINSAYIIKVVSKYTPTSDGELDIAQ
                     GTSMRTTDKYGYYNYAGYSNFIVTSNDTGGGDGTVKPEEKLYKIGDYVWEDVDKDGVQ
                     GTDSKEKPMANVLVTLTYPDGTTKSVRTDANGHYEFGGLKDGETYTVKFETPTGYLPT
                     KVNGTTDGEKDSNGSSVTVKINGKDDMSLDTGFYKEPKYNLGDYVWEDTNKDGIQDAN
                     EPGIKDVKVTLKDSTGKVIGTTTTDASGKYKFTDLDNGNYTVEFETPAGYTPTVKNTT
                     ADDKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKD
                     VTVTLQNEKGEVIGTTKTDENGKYRFDNLDSGKYKVIFEKPAGLTQTVTNTTEDDKDA
                     DGGEVDVTITDHDDFTLDNGYFEEDTSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDAGKHTPVKPMSTTKDHHNKAKALPETGSENNGSNNATLFGGLFAALGSLLLF
                     GRRKKQNK"
     gene            3595..5067
                     /locus_tag="O0293_002170"
     CDS             3595..5067
                     /locus_tag="O0293_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCY0281041.1"
                     /translation="MNYILGTILESKITGVEKAQINRLKLFKQHGISSKCVYVKWNPY
                     SYTYAKQHQIENDVFTMYDYFQKAINYKKTKQVNWIQYWEKSCRYTLKFVENSNDVRI
                     YDEEQFVMYAHFLDKQYHQLNYVNYFDHKRRKVKRELYDGRGFLSCSRILGEGQRIVL
                     ENYYTPNGEIVIQKYFDDIKGKNTLTKVILNEDQHQQFFDTEDELVQYFLHQLCKNND
                     QIILDRPHELGNVIAGLNQSIPVIVVLHSTHLSGAGNGIKSFYKTVFNNLTRYKAIVV
                     STEKQCQDISQYIENKIPVINIPVGYVANLKYQFDINQKEKNHIISIARLVENKQIKH
                     QIEVIKQLVTKHPNIQLNIYGHGNGLSEYRQLVEDYHLSEHVKFHGFKTHINEEIAKA
                     ELMLSTSKMEGFGLAILESLSVGTPVISYDVDYGPSELIQDGFNGYLVPQGDINQMVE
                     KVDQLLNNTQKLQQFSINSIESAQQYNATTISTKWQNILN"
     gene            complement(5191..6681)
                     /locus_tag="O0293_002171"
     CDS             complement(5191..6681)
                     /locus_tag="O0293_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCY0281042.1"
                     /translation="MNYFVGNSLGVNLTGIEKAIINRLNLFKEMGRPAQCVFLSWNRY
                     LYRNAQNYITSSDYINMYDFFQEATYLERNEPFDWLSYWTDECHYTLKHVENSHDFRI
                     YDQERFLMYAHFQDPKYRILDYVNHFDSQRRKVKRDFYDVRGFLSCSRILVDKQQTLC
                     EFFYNPEGDTKLEKYFSYKDGKPEVQKIIVYYANKQYFFNNETELGAFFIKQLYQHGD
                     LFFSDRNVYTAPIFNLTPESIPVVAVLHSTHIKNIDALDSSPFKNVYKAMFENLSRYR
                     AIIVSTEQQKLDVEKRINHTIPVVNIPVGYSETIDTPVQTLDQRSVKLISVARYSPEK
                     QLHQQIELIKRLVSYVPKIELHMYGFGSESKKLNELIQKYGLENHVYLRGFLSNLDQE
                     YSDAYLSLITSNMEGFSLALLESLAHGVPVISYDIKYGPNELITSDFNGYLITKNDED
                     ALFDKVKYVIDHPEVQQRLSKGSLAKAQQYSKASLIKQWDQFVRLI"
BASE COUNT         2600 a         1059 c         1125 g         1913 t
ORIGIN      
        1 aggagattta tatgattaac agggataata aaaaggcaat aacaaaaaag ggtatgattt
       61 caaatcgctt aaacaaattt tcgattagaa agtatactgt aggaactgca tcgattttag
      121 taggtacgac attgattttt ggtctaggga accaagaagc taaagctgct gaaaacacta
      181 gtacagaaaa tgcaaaacaa gatgatgcaa cgactagtga taataaagaa gtagtgtcgg
      241 aaactgaaaa taattcgaca acagaaaata attcaacaaa tccaattaag aaagaaacaa
      301 atactgattc acaaccagaa gctaaaaaag aatcaacttc atcaagtact caaaaacagc
      361 aaaataacgt tacagctaca actgaaacta agcctcaaaa cattgaaaaa gaaaatgtta
      421 aaccttcaac tgataaaact gcgacagaag atacatctgt tattttagaa gagaagaaag
      481 caccaaataa tacaaataac gatgtaacta caaaaccatc tacaagtgaa ccatctacaa
      541 gtgaaattca aacaaaacca actacacctc aagaatctac aaatattgaa aattcacaac
      601 cgcaaccaac gccttcaaaa gtagacaatc aagttacaga tgcaactaat ccaaaagaac
      661 cagtaaatgt gtcaaaagaa gaacttaaaa ataatcctga gaaattaaaa gaattggtta
      721 gaaatgatag caatacagat cattcaacta aaccagttgc tacagctcca acaagtgttg
      781 caccaaaacg tgtaaacgca aaaatgcgct ttgcagttgc acaaccagca gcagttgctt
      841 caaacaatgt aaatgattta attaaagtga cgaagcaaac aatcaaagtt ggcgatggta
      901 aagataatgt ggcagcagcg catgacggta aagatattga atatgataca gagtttacaa
      961 ttgacaataa agtcaaaaaa ggcgatacaa tgacgattaa ttatgataag aatgtaattc
     1021 cttcggattt aacagataaa aatgatccta tcgatattac tgatccatca ggagaggtca
     1081 ttgctaaagg aacatttgat aaagcaacta agcaaatcac atatacattt acagactatg
     1141 tagataaata tgaagatata aaatcacgct taactctata ttcgtatatt gataaaaaaa
     1201 cagttccaaa tgagacaagt ttgaatttaa catttgctac agcaggtaaa gaaacaagcc
     1261 aaaatgtcac tgttgattat caagatccaa tggtccatgg tgattcaaac attcaatcta
     1321 tctttacaaa attagatgaa gataagcaaa ctattgaaca acaaatttat gttaacccat
     1381 tgaaaaaatc agcaaccaac actaaagttg atatagctgg tagtcaagta gatgattatg
     1441 gaaatattaa actaggaaat ggtagcacca ttattgacca aaatacagaa ataaaggttt
     1501 ataaagttaa ctctgatcaa caattgcctc aaagtaatag aatctatgat tttagtcaat
     1561 acgaagatgt aacaagtcaa tttgataata aaaaatcatt tagtaataat gtagcaacat
     1621 tggattttgg tgatattaat tcagcctata ttatcaaagt tgttagtaaa tatacaccta
     1681 catcagatgg cgaactagat attgcccaag gtactagtat gagaacaact gataaatatg
     1741 gttattataa ttatgcagga tattcaaact tcatcgtaac ttctaatgac actggcggtg
     1801 gcgacggtac tgttaaacct gaagaaaagt tatacaaaat tggtgactat gtatgggaag
     1861 acgttgataa agacggtgtt caaggtacag attcaaaaga aaaaccaatg gcaaacgttt
     1921 tagttacatt aacttacccg gacggtacta caaaatcagt aagaacagat gctaatggtc
     1981 attatgaatt cggtggtttg aaagacggag aaacttatac agttaaattc gaaacgccaa
     2041 ctggatatct tccaacaaaa gtaaatggaa caactgatgg tgaaaaagac tcaaatggta
     2101 gttcggttac tgttaaaatt aatggtaaag atgatatgtc tttagatact ggtttttaca
     2161 aagaacctaa atacaactta ggtgactatg tatgggaaga tactaataaa gatggtatcc
     2221 aagatgcaaa tgagccagga atcaaagatg ttaaggttac attaaaagat agtactggaa
     2281 aagttattgg tacaactact actgatgcct cgggtaaata taaatttaca gatttagata
     2341 atggtaacta tacagtagaa tttgaaacac cagcaggtta cacgccaacg gttaaaaata
     2401 ctacagctga tgataaagat tctaatggtt taacaacaac aggtgtcatt aaagatgcag
     2461 ataatatgac attagacagt ggtttctata aaacaccaaa atacagttta ggtgattatg
     2521 tttggtacga cagtaataaa gacggcaaac aagattcaac tgaaaaaggt atcaaagatg
     2581 tgacagttac attgcaaaac gaaaaaggcg aagtaattgg aacaactaaa acagatgaaa
     2641 atggtaaata tcgtttcgat aatttagata gcggtaaata caaagttatt tttgaaaagc
     2701 ctgctggctt aacacaaaca gttacaaata caactgaaga tgataaagat gcagatggtg
     2761 gcgaagttga cgtaacaatt acggatcatg atgatttcac acttgataac ggatacttcg
     2821 aagaagatac atcagacagc gattcagact cagatagtga ctcagacagc gactcagact
     2881 cagacagcga ctcagactca gacagtgatt cagattcaga cagcgactca gattcagata
     2941 gcgactcaga ttcggacagc gattcagact cagatagcga ctcagattca gatagcgatt
     3001 cagactcaga cagcgactca gattcagata gcgattcgga ctcagacagc gattcagact
     3061 cagatagcga ctcagactca gacagcgact cagattcaga tagcgattcg gactcagata
     3121 gcgactcaga ttcagacagc gattcagact cagatagcga ctcagactca gacagtgatt
     3181 cagattcaga cagcgactca gactcagata gcgactcaga ttcggacagc gactcagact
     3241 ctgatagcga ctcagactca gacagtgatt cagacagcga ttcagactcg gatgcaggaa
     3301 aacatacacc tgttaaacca atgagtacta ctaaagacca tcacaataaa gcaaaagcat
     3361 taccagaaac aggtagtgaa aataacggct caaataacgc aacgttattt ggtggattat
     3421 ttgcagcatt aggttcatta ttgttattcg gtcgtcgcaa aaaacaaaac aaataataca
     3481 atatgaccca ggtccttgtg gcctggtttt tttataatta cacatgcaat agatgtattt
     3541 ttcatataaa ataaacaata aagatacgga ataaaactta tatgaggcga taatatgaat
     3601 tacattttag gaacaatttt agaaagtaaa attacaggtg tagaaaaagc gcaaataaat
     3661 agattgaagt tgttcaaaca acacggcata tcttcaaaat gtgtatatgt taaatggaat
     3721 ccttattcat acacatatgc gaagcaacat cagattgaaa atgatgtatt tacaatgtat
     3781 gactattttc aaaaagcaat caattataaa aagacaaagc aagttaactg gatacagtat
     3841 tgggaaaagt catgtaggta cacattgaaa tttgtggaaa attcaaatga tgtcagaata
     3901 tatgatgaag agcaatttgt aatgtatgct cattttttag ataaacagta tcatcaatta
     3961 aattatgtga attattttga tcataaaaga agaaaagtaa aacgcgaatt gtatgatgga
     4021 agaggctttt taagttgttc tcgaatttta ggtgaaggac aacggattgt actcgaaaat
     4081 tactatacac ctaatgggga aatcgtcatc caaaaatatt ttgacgatat aaaagggaaa
     4141 aacacgctca caaaggttat cttaaatgaa gaccagcatc aacaattttt tgatacagaa
     4201 gatgaattag ttcaatattt tctccatcaa ttatgtaaaa ataatgatca aatcatatta
     4261 gatcgtcctc atgaattagg aaatgttata gcgggattaa atcaaagtat tccagttatt
     4321 gttgtactcc atagtacaca tttatccggt gccggtaatg gtataaaaag tttttataaa
     4381 acagtgttta ataatttaac acgttataaa gcgattgttg tatcaacaga aaagcaatgc
     4441 caagatattt cacaatatat tgaaaataaa ataccagtta tcaatattcc ggttggctac
     4501 gtggcaaatt taaagtatca atttgacatc aatcaaaagg agaaaaatca tatcatatca
     4561 attgctcgcc tcgttgaaaa taaacaaatt aaacatcaaa ttgaagtaat caagcaatta
     4621 gtaacaaaac atcccaatat tcaattgaat atttatggac atggaaatgg tttgtcagaa
     4681 tatcgacaac ttgtagaaga ttatcattta tcggaacatg ttaaatttca tggttttaag
     4741 acgcatatta atgaagagat tgctaaagca gaactgatgt tatcgacaag taaaatggaa
     4801 ggttttggct tagcaatttt agagtcgctt tcagtaggta caccagtgat cagttatgat
     4861 gtagattatg gtccatcaga actgattcaa gatggattta atggctattt agtacctcaa
     4921 ggtgacatca atcaaatggt tgaaaaggtc gaccaattac taaataatac tcaaaaattg
     4981 caacagtttt caattaatag catagaatct gcacaacagt acaatgcaac tactatcagt
     5041 acaaagtggc aaaatatttt aaactaagtc aaaagaaaaa agcattttcc tagaatcatc
     5101 tatttaacaa acctcgttca tacgaactta catgtttagt taaatgacat aggaaaatgc
     5161 tatttttgta aaaataaagg taaagtacat ttatataaga cgaacaaatt ggtcccattg
     5221 tttaattaac gacgctttac tatattgttg tgcttttgcc aaactacctt ttgacagtcg
     5281 ttgctgtact tcaggatgat caatcacata ttttacttta tcaaataggg catcttcatc
     5341 atttttagta attaaataac cattgaaatc tgaagtaatc agttcgttag gtccatattt
     5401 aatatcataa ctaataactg gaacaccatg tgctaaagat tcaagtagcg ctaaagagaa
     5461 accttccatg ttacttgtta ttaaactcaa ataggcatcg ctatattctt ggtctagatt
     5521 gcttaaaaag ccgcgtaagt aaacatgatt ttccaatcca tatttttgta tcaattcatt
     5581 taatttttta ctttcagaac caaaaccata catatgaagc tctatttttg ggacatacga
     5641 tactaagcgt ttaattaatt caatttgttg atgtaattgt ttttcaggtg aataacgagc
     5701 aacggaaatt aatttaacac tgcgctgatc taatgtttgg actggtgtat caattgtttc
     5761 actatagccg acaggaatat taacaactgg aatagtatgg ttaatacgtt tttcaacatc
     5821 taatttttgc tgctcagtag aaacgataat tgcacgatat cgagataaat tttcaaacat
     5881 cgctttatat acatttttaa atggcgatga atctaatgca tcaatatttt taatgtgtgt
     5941 actgtgaagc acagctacta ctgggattga ctcaggcgtt aagttgaaaa taggtgctgt
     6001 gtacacatta cgatcactga aaaataaatc cccatgttga tatagttgtt taatgaaaaa
     6061 tgcgcctaat tccgtttcat tattaaagaa atattgtttg ttagcatagt aaacaataat
     6121 tttttgtact tctggtttgc catccttgta agaaaaatac ttttctaatt ttgtgtcacc
     6181 ttctggatta tagaaaaatt cacataatgt ttgttgttta tcaacaagaa tcctactaca
     6241 acttaaaaag ccacgcacat cataaaaatc acgttttact tttcgtcttt gactatcaaa
     6301 atgatttaca taatctaata tacgatattt aggatcttga aaatgggcat acattaagaa
     6361 acgctcttga tcatatattc taaagtcatg actattttca acatgtttta aagtataatg
     6421 acattcatca gtccaatacg acaaccagtc aaatggttca ttgcgttcta aatatgttgc
     6481 ttcttggaag aaatcataca tattaatata gtcagaacta gtaatataat tttgggcatt
     6541 tctatataaa tatctattcc atgacagaaa tacacattgc gctggtcttc ccatttcttt
     6601 aaataaattt aaacgattaa taattgcttt ctctatccca gttaaattaa cacctaaact
     6661 attacctaca aaataattca tttacaacac cacttat
//
LOCUS       DAKTIO010000143         6633 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.144, whole genome shotgun sequence.
ACCESSION   DAKTIO010000143 DAKTIO010000000
VERSION     DAKTIO010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6633
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.144"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            234..554
                     /locus_tag="O0293_002172"
     CDS             234..554
                     /locus_tag="O0293_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF961 family protein"
                     /protein_id="HCY0281043.1"
                     /translation="MKPVLDIKKTLGQLNFLGVDEKYKYENGERTDKTVYAYKLASQE
                     QGEQITVKTPNKVELDYLQECELVEPDVKMYVQMSGDFGTIAYSWNAEDIKVVGSNSA
                     LKQK"
     gene            568..870
                     /locus_tag="O0293_002173"
     CDS             568..870
                     /locus_tag="O0293_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281044.1"
                     /translation="MDYYTADRLYRYTNSSNLSEPILNYVASRINWGDKVSLMTLAKE
                     IQSKFNDSYVKENTVKGRPKIYADLCLLCMSLSEAGHGRMLQVNLEDCIYIGDIDV"
     gene            1045..2136
                     /locus_tag="O0293_002174"
     CDS             1045..2136
                     /locus_tag="O0293_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000172946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCY0281045.1"
                     /translation="MTLKNQCTPLTNRGVERTNKSAVEAVVDWVQVTFHIAPISAVIE
                     DVIGLPITLFKKRNSGIYFYNRGYEFSNIKLYYSSDDESMGIHLQLTGTGCREFEHHL
                     QQLNKTWQDFFDKCLSVNANFTRIDIAIDDYKTYLKVPLLIKKAEKAECVSRFRAGSA
                     INGFNLSDGRSKGATFYIGSKQSNLYCRFYEKNYEQAFKRHCDVEDIGLWNRYEIQMR
                     KAYAVNCAKVLSRTDNISEIVKSILHNNLRFISPPKGGNDKNRKRWPLYRPWALFIKD
                     TEKLNLTTRPTLKSIEDNLDWLCKQVATTLDTVLTAESMAQSEGLLTDTDFLDKILAH
                     SQFNDEHTNRINHYLEALKQKKHLSKDKC"
     gene            2197..3252
                     /locus_tag="O0293_002175"
     CDS             2197..3252
                     /locus_tag="O0293_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCY0281046.1"
                     /translation="MKIISKYILTRFSKNGKHFDIPKEQRRVIPKFKNRRKMIVICFY
                     SLLAILLILLLASFIKATRANNDSKEAVNKTNMIQKKYEDNANTVQYSPKLKLYADKF
                     IDTYMNISKDSKELESREKELLKYFPSDYKKPDEKISDTERKLNSKEFYNIKRKDKQT
                     IIQYIVNYDVNITEKKEVKVKKKKKSEKDKDEYETKTEEKQRKVNQNILINIPIKSEN
                     NKYVVVEYPYFTPIPDSQLNQAKMVEDNLKDNKREDNPKAKAFIEDFFNKYASSKSDD
                     MAYLMDNPEGLEGTREVSQIREIRLYPKGDDYVAKVEILMKDKDSPLENLEHYTLDIT
                     KKDGKYYVKNMTNSIGG"
     gene            3257..3517
                     /locus_tag="O0293_002176"
     CDS             3257..3517
                     /locus_tag="O0293_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281047.1"
                     /translation="MSGLFNFFVLGADRPSLGGVGDWISNEVGTGIGLVVLVVGIVKW
                     ASGKYGHMVILFVVGGFLFLVSKGPEQVFNAIAGVWKMIFGG"
     gene            3529..3912
                     /locus_tag="O0293_002177"
     CDS             3529..3912
                     /locus_tag="O0293_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCY0281048.1"
                     /translation="MDKKAYNLKRTFEQPIVMYEFTDKFRINKGFRLDFWATFLIVWF
                     ILFLLFWYLLQPVIMSIGGLMFIYFTVAPYYITKYIVKLKQDGKKLFFFLWDFVIFVF
                     NVQLRKVKLSYDEEVEYHDKKITFK"
     gene            3947..6442
                     /locus_tag="O0293_002178"
     CDS             3947..6442
                     /locus_tag="O0293_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCY0281049.1"
                     /translation="MNLKAPYAGLRDNLLLTKTGEVWAYYRVRSESISTANYDAKEES
                     KKRMRYFLESIKGYQDFHFEMYDHDLDLRNKFKEISKDFDDEAFNVAEYYSKETIDVL
                     EQELQMITHNSFVMGVKIRELADDAVTLKEILKSTLSDTAERIVSNFGFDVNLDDKIL
                     DKYKTFERDLADSFLGIGGERLTENELAYIIRKPFISNATHDVQEESSRRAITDIYNA
                     VIDPTRLSVLEVENDNEKFYTTTVVVDDFPLDMEYTHLFEKAQNMPFPVECHVKAKII
                     NNEKVKKKFDRAKINHKQQAKEKQDTGDSASEDVQDNLFVLNQMEREVTGSGVFIEWV
                     FMFVIKSDDYRDCKRKAKRLMKRLKETQIYAVNPLADQLQLFYQCLHGNDLFINKYWL
                     QRTTATGIAENLFGVSQSLGTKTGFYIGRIDKFIRSVSREEAVASSRDIILFSLLLAA
                     KGIKGAVSDSPHVLITGQTGKGKSFLAKLLWIYTSFFKGQMLYWDPKSEFAEWFDRVT
                     ESKEMQEKYPLFINHLKTFKYVTLNYEDSTNYGCLDPIVFLDGIEANEMLKSVFDEIK
                     SFENYHHIETAIYQAISDTVEERENGKKVGSLNVIEKLQNNEDEHVKNAGDLLFEKTQ
                     NNILKLVFSDGTNPALNIQEKATILQVKGLDMPKADDDTSSYSTSEKNGITLMLLIGK
                     FLEKFGSRRDVQTTIFIDEGWAFSASRQGKKVGKRIKRTGRSENNSLVFITQSVKDKA
                     DDDGGNFGCHFAFDEKDEREDILKSLGLEYSKESPENMEMLKDLKKGQCIFSDFYGRV
                     GKMVVHCPFEEMTEAFRTQEDSASSKAEEKFAM"
     gene            6496..>6633
                     /locus_tag="O0293_002179"
     CDS             6496..>6633
                     /locus_tag="O0293_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HCY0281050.1"
                     /translation="MAMNEIALFKGVRIQPYQKYIDFMFASVLAFIFLVYRIYILFID
                     YK"
BASE COUNT         2395 a          718 c         1313 g         2207 t
ORIGIN      
        1 atggtgtgtc taatacattt atttgtaaga tgtatcatat ttcaattgaa gacttagaat
       61 tttttgaggt gatgaaccga taggtttgat taaaaatatg ataggtcagg agaagcatag
      121 acactccatt atcaaaaatg cagtggtacg gatttgcggg agttattcct attcgtgcga
      181 ccctttagaa gtcgtttaat tttattaaaa attttgtaaa ggatggatat attatgaaac
      241 cagtattgga tattaaaaaa actttaggac aattaaactt tttaggtgta gatgaaaaat
      301 ataaatatga aaatggagag cgtactgata aaactgttta tgcatataaa ttagcgagtc
      361 aagaacaagg cgaacaaatc actgttaaaa cgcctaataa agtggaatta gactatttac
      421 aagaatgcga attagttgaa ccagacgtta aaatgtatgt tcaaatgtca ggcgattttg
      481 gaactatcgc atatagctgg aatgcagaag atattaaagt agttggttct aatagtgcac
      541 ttaaacaaaa ataattaggt gatgtaaatg gattattata cagcagatag attatatcgc
      601 tacacaaata gttcaaattt gagtgaacct attttaaact atgttgctag tcgtataaat
      661 tggggcgata aagtttcatt aatgacttta gctaaagaaa tacagtctaa atttaatgat
      721 agttacgtta aagaaaatac ggtaaaaggt cggcctaaaa tttacgctga cctttgtcta
      781 ttgtgtatga gcttgagtga agcaggacat ggcagaatgc tacaagtgaa tttggaagat
      841 tgtatttata ttggcgatat tgatgtttaa catatagtat tagtgattta acatgaccgt
      901 gtagctttta gcgtatgcag gcgcagaaat gcaagcacgg agtgcgttaa gcatttgggc
      961 ggagcctgcg acaaatacgc gttaaaaaag cgagaacggt catgtatcaa atcgcttgcg
     1021 atttgatagg aggcataaat aaatatgact ttaaaaaacc agtgtacccc ccttactaat
     1081 aggggggtag agcgtacaaa taaaagtgct gtggaggcag ttgttgactg ggttcaggtc
     1141 actttccata tagcccctat ttcagcagta attgaggacg ttatcggttt gcctataacg
     1201 ttgttcaaaa aacgaaatag tggcatttac ttttataatc gcggttatga attttcaaac
     1261 attaaattgt attattcaag tgatgatgaa tcaatgggca ttcacttaca gttaactggt
     1321 acaggttgta gagagtttga acatcattta cagcagttga ataaaacttg gcaagatttt
     1381 tttgacaaat gtttatcagt taatgcaaat tttacaagaa ttgatattgc aattgatgat
     1441 tataaaacat atttaaaagt gcctttatta attaaaaagg ctgaaaaagc tgagtgtgtt
     1501 tcacgtttta gagctggaag cgcaataaac ggttttaatt tgtcagacgg aagaagtaaa
     1561 ggcgctactt tttatattgg ttctaaacaa agcaatttat attgtcgttt ttatgaaaag
     1621 aattatgaac aagcgtttaa acgtcattgt gatgtcgaag acattgggct atggaatcgt
     1681 tatgaaatac aaatgcgaaa ggcatatgca gttaattgtg cgaaagtgtt gagtagaaca
     1741 gataatataa gcgaaattgt aaagtctata ttgcataata atttgcgttt tatctctcct
     1801 cctaaaggtg gaaatgataa aaatcgtaag cggtggccat tatatagacc ttgggcgtta
     1861 tttattaaag atactgaaaa attgaattta actactagac caacattaaa gtccattgag
     1921 gataatcttg attggttatg taaacaagtt gctactactt tagatacagt gttaactgcc
     1981 gaaagtatgg cgcaatctga ggggttgctt acagatactg attttttaga taaaatttta
     2041 gcgcattcac aatttaatga tgagcatacg aatagaatta atcattattt ggaggcttta
     2101 aaacaaaaaa agcacttatc aaaagataag tgttaaaaaa ataactggga tgtgattttt
     2161 attaaaaatt tcgtaaagga ttttagaaaa tcgtttatga aaataataag caagtacatt
     2221 ttaacacgat tttctaaaaa cggaaagcat tttgatattc ctaaagaaca acgccgagta
     2281 attccaaaat ttaaaaatcg tcgtaagatg attgtaattt gtttttactc attattagct
     2341 attttgttaa ttcttttatt agcttctttt attaaagcta caagagcaaa taatgacagt
     2401 aaagaagcag ttaataaaac gaatatgatt caaaaaaagt atgaagataa tgcgaataca
     2461 gttcagtata gtcctaaatt aaagttatat gctgataagt ttattgatac gtatatgaat
     2521 atctcgaaag attcaaaaga attggaatct agagaaaaag aattgttgaa atattttcca
     2581 tcagattata aaaaacctga tgaaaaaatt tcagatacag aacgaaaatt gaatagtaaa
     2641 gaattttata atattaaacg taaagataaa caaacaatta tacagtatat tgttaattat
     2701 gatgtgaata ttactgaaaa gaaagaagtt aaagttaaga agaaaaagaa aagtgaaaaa
     2761 gataaagatg agtatgaaac aaaaacagaa gaaaagcaac gtaaagtaaa tcaaaacatt
     2821 ttaataaata tacctattaa gagtgaaaat aataagtatg ttgttgttga atatccttat
     2881 tttacgccaa tccctgatag tcaattgaat caagctaaaa tggttgagga taatttgaaa
     2941 gacaataaac gggaagacaa tccgaaagca aaagctttta ttgaagattt ctttaataaa
     3001 tatgcttcta gtaagtctga tgatatggcc tatttaatgg ataaccctga gggcttagaa
     3061 ggaactagag aagtttctca gataagagaa attaggttat atccaaaagg cgatgattat
     3121 gttgctaaag ttgaaatttt gatgaaagat aaagattctc ctttggagaa tttggagcat
     3181 tacacattag atataacaaa aaaagatggt aaatattacg ttaaaaatat gactaattct
     3241 attggaggtt aatgttatga gtggtttatt taattttttt gttttaggtg ctgaccgtcc
     3301 atcacttggt ggagttggtg actggataag taatgaagta ggtacgggta ttggtttagt
     3361 agtattggtt gttggtattg taaaatgggc tagtggtaaa tatgggcata tggttatatt
     3421 atttgttgtt ggtggctttt tattcttagt ttctaaaggt cctgaacagg tatttaatgc
     3481 gattgcaggt gtttggaaaa tgatttttgg cggttgaggt gattccattt ggataaaaaa
     3541 gcttataatt taaagcgaac ttttgaacaa ccgatagtta tgtatgaatt tacagataag
     3601 tttcgtatta ataagggttt tcgtttagat ttttgggcta catttttgat tgtgtggttt
     3661 atattatttt tgttattttg gtatttatta cagcctgtaa ttatgtcgat tggtggactt
     3721 atgtttattt actttacggt tgcaccttat tatataacga aatatattgt taagttaaaa
     3781 caagatggaa agaaattatt tttcttctta tgggattttg taatatttgt ttttaatgtg
     3841 cagttaagaa aagttaaatt aagttatgat gaagaagtag aatatcatga taaaaaaata
     3901 acatttaaat agcccctatt ggggttattt ttattggagg gatgacatga atttaaaagc
     3961 accgtatgca ggtttaagag ataatttgtt attaacgaaa actggcgaag tttgggcgta
     4021 ttatcgtgtt cgttctgagt ctatatcaac agcaaattat gatgcaaaag aagaatcaaa
     4081 aaagcgtatg cgttattttt tagagtcgat taaaggttat caagattttc attttgaaat
     4141 gtatgaccac gatttagatt tgcgaaataa atttaaagaa attagtaaag attttgatga
     4201 tgaagctttt aatgtagctg aatattattc aaaagaaact attgacgtat tagaacaaga
     4261 attacaaatg attacgcata attcttttgt tatgggtgtt aaaatacgtg aattagctga
     4321 cgatgcagtt acacttaaag agattttaaa aagtacatta tcagatacgg ctgagcgtat
     4381 tgtttcgaat tttggttttg atgtgaattt agacgataaa attttagata agtataaaac
     4441 atttgaacga gatttggctg atagttttct aggtattgga ggcgagcgtt taactgagaa
     4501 tgaattggct tatattatta gaaagccttt tatttcaaat gcaacacatg acgtacagga
     4561 agaatcttca agaagagcta ttacagatat ttataatgca gtaattgacc ctactcgctt
     4621 gagtgtttta gaagttgaaa atgataacga gaaattttat acaacaacag ttgttgttga
     4681 tgattttccg ttagatatgg aatatacaca tttatttgag aaagctcaaa atatgccttt
     4741 tccggttgaa tgccatgtaa aagcaaaaat cattaataat gagaaagtta agaaaaaatt
     4801 tgaccgtgcg aaaataaatc ataaacaaca agcaaaagaa aagcaagata ctggcgacag
     4861 tgcttcggaa gatgttcaag ataatttgtt tgtattgaat caaatggaac gagaagtgac
     4921 tggctcaggt gtatttattg agtgggtatt tatgtttgta attaaaagtg atgattatag
     4981 agattgtaaa cgtaaagcaa agcgtttgat gaaacgatta aaggaaactc aaatatatgc
     5041 agttaatcca ttagctgacc agttacaatt gttttatcaa tgtttacatg gtaatgattt
     5101 atttattaat aagtattggt tacaaagaac aacggcaaca ggtatagcag agaatttatt
     5161 tggtgtgtct caaagtttgg gaacaaaaac gggtttttat atagggcgta ttgataaatt
     5221 tatacgttct gtttcacgtg aggaagctgt tgcaagttca agggatataa ttttgtttag
     5281 tttattgttg gccgcaaaag gtattaaggg ggctgtcagt gatagtccgc atgttttaat
     5341 tactggtcaa actgggaaag gtaaatcatt tttagcaaaa ttattatgga tttatacatc
     5401 attttttaaa ggtcaaatgt tgtactggga tccaaaatct gagtttgcag aatggtttga
     5461 cagagtaaca gaaagtaagg aaatgcaaga aaagtatccc ctatttatta atcatttaaa
     5521 aacatttaaa tatgtaacat tgaattatga agattcaacc aattatggtt gtctagaccc
     5581 tattgtattt ttagatggta ttgaagctaa tgagatgttg aaatcagtgt ttgatgaaat
     5641 taaatctttt gaaaattatc atcatattga aacagcaatt tatcaagcta tttctgatac
     5701 ggttgaagaa cgtgaaaatg gtaaaaaagt tggttcatta aatgtaattg aaaaattaca
     5761 gaataatgaa gatgagcatg taaaaaatgc aggcgattta ttatttgaaa aaacgcaaaa
     5821 caatatttta aaacttgttt ttagtgatgg tacgaatcca gctttaaata ttcaagaaaa
     5881 agcaactatt ttacaggtta aaggtttaga tatgcctaaa gcagatgatg atacatcaag
     5941 ttattcaaca agtgagaaaa atgggattac attaatgtta ttaattggta agtttttaga
     6001 gaaattcggt tctcgtagag atgttcaaac gactatattt attgatgagg gttgggcttt
     6061 tagtgcttca agacaaggta aaaaagttgg taagcgtata aaaagaacgg gacgttctga
     6121 gaataattca cttgtattta ttacacaatc ggtaaaagat aaagcagatg atgatggtgg
     6181 taactttggt tgtcattttg catttgatga aaaagatgaa cgtgaggata ttttaaaatc
     6241 tttaggctta gaatattcaa aagaatctcc tgaaaacatg gagatgttga aagatttgaa
     6301 gaaaggtcaa tgtatattca gtgattttta tggacgtgtt ggaaaaatgg ttgtacattg
     6361 tccatttgag gaaatgactg aagcatttag aacacaagaa gattctgcaa gctctaaagc
     6421 tgaagaaaaa tttgcgatgt aatttttagc acatacaaaa ttgtatgtgc tttttatttt
     6481 aaaggagtgg atttgatggc aatgaatgaa attgctttat ttaaaggcgt acgtatacag
     6541 ccttatcaga agtatattga ttttatgttt gcaagtgtat tagcatttat ttttttggta
     6601 tatcgtatat atattttgtt tattgattat aaa
//
LOCUS       DAKTIO010000144         6561 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.146, whole genome shotgun sequence.
ACCESSION   DAKTIO010000144 DAKTIO010000000
VERSION     DAKTIO010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6561
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.146"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            18..815
                     /locus_tag="O0293_002180"
     CDS             18..815
                     /locus_tag="O0293_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HCY0281051.1"
                     /translation="MRIMFIGDIVGKIGRDAIETYIPQLKQKYKPTVTIVNAENAAHG
                     KGLTEKIYKQLLRNGVDFMTMGNHTYGQREIYDFIDEAKRLVRPANFPDEAPGIGMRF
                     IQINDIKLAVINLQGRAFMPDIDDPFKKADQLVKEAQEQTPFIFVDFHAETTSEKYAM
                     GWHLDGRASAVVGTHTHIQTADERILPKGTGYITDVGMTGFYDGILGINKTEVIERFI
                     TSLPQRHVVPNEGRSVLSGVVIDLDKEGKTKHIERILINDDHPFSTF"
     gene            955..2715
                     /locus_tag="O0293_002181"
     CDS             955..2715
                     /locus_tag="O0293_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HCY0281052.1"
                     /translation="MKPQLSWKVGGQQGEGIESTGEIFATAMNRKGYYLYGYRHFSSR
                     IKGGHTNNKIRVSTTPVHAISDDLDILIAFDQETIDVNHHEMREDSIILADAKAKPVK
                     PEGCHAQLIELPFTATAKELGTALMKNMVAIGATSALMNLNTNTFEELITNMFSKKGD
                     KVVEVNIQALNEGYQLMQSRLPEIYGDFELESTDALPHLYMIGNDAIGLGAIAAGSQF
                     MAAYPITPASEVMEYMIANISKVNGAVIQTEDEIAAVTMAIGANYGGVRAFTASAGPG
                     LSLMMEAIGLSGMTETPLVIINTQRGGPSTGLPTKQEQSDLMQMIYGTHGDIPKIVVA
                     PTDAEDAFYLTMEAFNLAEQYQCPVIVLSDLQLSLGKQTVEKLDYNRIEIKRGEIIQS
                     DIEREEDDKGYFKRYALTSDGVSPRPIPGVKGGIHHITGVEHNEEGKPSESASNRQQQ
                     MEKRMRKIEQLLIESPVEANLQHEDADILYIGFISTKGAIQEGSNRLNQQGIKVNTIQ
                     IRQLHPFPTSVIQDAVNKAKKVVVVEHNYQGQLASIIKMNVNIHDKIENYTKYDGTPF
                     LPHEIEEKGKIIATEIKEMV"
     gene            2716..3582
                     /locus_tag="O0293_002182"
     CDS             2716..3582
                     /locus_tag="O0293_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HCY0281053.1"
                     /translation="MATFKDFRNNVKPNWCPGCGDFSVQAAIQKAAANIGLEPEEVAI
                     ITGIGCSGRLSGYINSYGVHSIHGRALPLAQGVKMANKDLTVIASGGDGDGYAIGMGH
                     TIHALRRNMNMTYIVMDNQIYGLTKGQTSPSSAVGFVTKTTPKGNIEKNVAPLELALS
                     SGATFVAQGFSSDIKGLTKLIEDAINHDGFSFVNVFSPCVTYNKINTYDWFKEHLTSV
                     DDIENYDSTDKQLATKTVIEHESLVTGIVYQDKETPSYESQIKELDDTPLAKRDIKIT
                     EDTFNALTEQFI"
     gene            3676..3969
                     /locus_tag="O0293_002183"
     CDS             3676..3969
                     /locus_tag="O0293_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HCY0281054.1"
                     /translation="MKDTLMSIQIIPKTPNNDNVIPYVDEAIKIIDESGLHFRVGPLE
                     TTVQGNMNECLILIQSLNERMVELECPSIISQVKFYHVPDGITIETLTEKYDE"
     gene            4103..5647
                     /gene="miaB"
                     /locus_tag="O0293_002184"
     CDS             4103..5647
                     /gene="miaB"
                     /locus_tag="O0293_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCY0281055.1"
                     /translation="MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKR
                     GKQEVRYNRDFQIDEKYRGMGNERTFLIKTYGCQMNAHDTEVIAGILEALGYQATTDI
                     NTADVILINTCAIRENAENKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILK
                     SYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVN
                     IMYGCDKFCTYCIVPFTRGKERSRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDL
                     QDIEYDLGDLLQAISKIAIPRVRFTTSHPWDFTDHMIDVISEGGNIVPHIHLPVQSGN
                     NAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTDIIVGYPNESEEQFEETLTLYDEV
                     GFEHAYTYLYSQRDGTPAAKMKDNVPLNVKKERLQRLNKKVGHYSQIAMSKYEGQTVT
                     VLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEVRIDEAKQYSLNGSFIKEVE
                     PEMVIQ"
     gene            5648..6013
                     /locus_tag="O0293_002185"
     CDS             5648..6013
                     /locus_tag="O0293_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
                     /protein_id="HCY0281056.1"
                     /translation="MYNKDDVLNQADNIANKIKNLDTIKTYQQIEAQIHQNQTIKTKM
                     DMLKKHQKQAVNFQNYGKQNALEQSEHTIQSIEAEINTLPIVEQFQTSQYEANQLLKM
                     FVSTMETRLNDHNKAKHSD"
     gene            6040..6531
                     /gene="thiW"
                     /locus_tag="O0293_002186"
     CDS             6040..6531
                     /gene="thiW"
                     /locus_tag="O0293_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HCY0281057.1"
                     /translation="MKSRKLAITALLIAINVVLSSIIIIPLGPVKAAPVQHFVNVLSA
                     VIVGPWYGLAQALISSILRVLFGTGTAFAFPGSMIGVLLASMFYIYRKHIFMAAVGEV
                     LGTGVIGSLICIPLAYFLGLQDFFIKPLMITFIVSSAIGSIISYFLLITLKKRGILQR
                     FIK"
BASE COUNT         2428 a          916 c         1265 g         1952 t
ORIGIN      
        1 gagaggatga acaaaacatg agaataatgt ttatagggga tatcgtaggt aaaattggac
       61 gagacgcaat tgaaacgtac atacctcaac tgaagcaaaa gtataaacca acagttacaa
      121 ttgtaaatgc tgaaaatgca gcacatggta aaggtttgac tgaaaaaata tataaacaat
      181 tactaagaaa tggtgtagat ttcatgacta tgggtaatca cacatatggt caacgtgaaa
      241 tttatgattt tatagatgaa gcaaaacgac tagtaagacc agcgaatttt ccggatgaag
      301 cgccgggaat tggtatgaga tttatacaaa ttaatgatat taaacttgca gttattaatc
      361 tgcaaggaag agcgtttatg ccagatattg atgatccttt taaaaaggca gatcaattag
      421 tcaaggaagc acaagaacaa actccgttta tatttgttga ttttcatgca gaaacaactt
      481 ctgaaaagta tgcaatggga tggcatttag atggtagagc tagcgctgtt gttggaacgc
      541 atacacacat tcaaacagca gatgaacgta ttttaccaaa ggggacaggg tatataacgg
      601 atgttggtat gacaggtttt tatgatggca ttttaggaat aaataaaaca gaggtaattg
      661 agcgttttat cactagtttg ccacaaagac atgttgttcc aaatgaaggt agaagtgtat
      721 tatctggtgt tgttattgat ttagacaaag aaggtaaaac aaagcacatc gaacgtatat
      781 tgataaatga tgaccatcca ttttcaacat tttaaaatta cgtaagtaaa cattcgaatt
      841 ggaccctatc gtccattagt atgaatttaa tatagtacca ctgtttacat agtaaatcgg
      901 tggttctttt tgttatcatt taatatgaaa tatatccata ggaggcatat aactatgaaa
      961 ccacaattat cgtggaaagt tggcggtcaa caaggcgaag gtattgaatc aactggggaa
     1021 atcttcgcta cggctatgaa tagaaaagga tattatttat atggatatag acatttttca
     1081 agtcgtatca aaggtggaca tacgaataat aaaattagag tttctacgac gcctgttcat
     1141 gcaattagtg atgatttaga tattttgatt gcatttgacc aagaaacaat tgatgttaac
     1201 catcatgaaa tgagagaaga cagtattatt ttagctgatg ccaaggctaa acctgtgaaa
     1261 ccagaaggat gtcatgcaca gcttattgaa ttacctttta cagcaaccgc taaagaatta
     1321 ggtacagcat taatgaaaaa catggttgca ataggtgcta ctagcgcatt gatgaatttg
     1381 aatacaaata catttgaaga acttattact aatatgtttt ctaaaaaagg tgacaaggta
     1441 gttgaagtca atatccaagc attaaacgaa ggttatcaat taatgcaatc tcgcttacct
     1501 gaaatctacg gggactttga attagagtca acagatgcac taccacatct atatatgatt
     1561 ggtaacgatg ccattggatt aggtgcaatt gctgcaggtt cacaatttat ggcggcatat
     1621 cctattacac ctgcgtctga agttatggaa tatatgattg ccaatatatc taaagtaaac
     1681 ggagcggtta ttcaaacaga agatgaaatt gctgctgtaa ctatggctat tggtgcaaat
     1741 tatggtggag tcagagcgtt tacggctagt gctggtccag gtttatcttt aatgatggaa
     1801 gcaattggat tatctggtat gactgaaacg ccattagtca ttattaatac ccaacgaggt
     1861 ggaccttcta ctggattacc tacgaaacaa gaacagtcag atttaatgca aatgatttat
     1921 ggtacacatg gtgatattcc aaaaattgtt gtagcaccaa cagatgcaga agatgcattt
     1981 tatttaacta tggaagcatt taatttagca gaacaatatc aatgccctgt tatagttcta
     2041 agtgatttgc aattatcttt aggtaaacaa actgttgaaa aattagatta taatcgtatt
     2101 gaaattaaac gtggtgaaat cattcaatct gatattgaac gtgaagaaga tgataaaggt
     2161 tatttcaagc gttatgcgtt aacatccgat ggtgtttctc ctagacctat ccccggtgtt
     2221 aaaggaggta ttcatcatat aactggtgtg gaacacaatg aagaaggtaa acctagtgaa
     2281 tctgcgtcaa atagacaaca acaaatggaa aaacgaatgc gtaaaattga gcagttacta
     2341 attgaatcgc cagtagaagc taacttacaa catgaggatg cagatattct ttatatcggt
     2401 tttatttcta caaaaggtgc aattcaagaa ggtagtaacc gtttgaatca acaaggcata
     2461 aaagttaaca ctatacaaat tagacaattg catccattcc caacaagcgt tattcaagat
     2521 gcagttaata aagcgaagaa agtcgttgta gtggagcaca attatcaagg acaattggct
     2581 agtattataa aaatgaatgt caatattcat gataagattg aaaattatac aaagtatgat
     2641 gggacacctt tcctaccaca tgaaatcgaa gaaaaaggca aaataattgc tactgaaata
     2701 aaggagatgg tatagatggc gacatttaaa gattttagaa ataatgttaa gcctaactgg
     2761 tgccccggat gtggcgattt ctcagtacaa gctgcaattc aaaaagcagc cgcaaatata
     2821 gggttagaac ctgaagaagt agctatcatc accggtatag gatgttctgg ccgtctttca
     2881 ggatatatta attcttatgg cgttcattct attcacggac gtgcattacc tttagctcaa
     2941 ggtgtaaaaa tggcgaataa agatttaact gttattgcat cgggaggaga tggtgatggt
     3001 tatgctatag gtatggggca tacaatccat gctttaagaa gaaatatgaa catgacgtat
     3061 atagtcatgg ataatcaaat ttatggtttg acaaagggac aaacatcgcc gtcatcagca
     3121 gtaggatttg ttactaaaac aacgccaaaa ggtaatatag aaaaaaatgt tgcgccttta
     3181 gaattagcat tatcatctgg tgccacattt gtagcccaag gtttttcaag cgatattaaa
     3241 ggattaacaa aactaattga agatgcaatt aatcatgatg gattttcatt cgttaatgtc
     3301 ttttcaccat gtgtgactta taataaaatt aacacatacg attggtttaa agaacattta
     3361 acaagtgttg atgacattga aaattatgat tctacagata aacaattagc gactaaaact
     3421 gttattgaac atgaatcttt agtaactggt attgtttatc aagataaaga aacaccatca
     3481 tatgaatctc aaattaaaga gttagatgat acaccacttg ctaaaagaga tatcaaaatt
     3541 actgaagaca ccttcaatgc attaactgaa caatttattt aataattaaa actgagtcgt
     3601 ataaatgtat ttataacaga tccatttatg ctactcagtt ttttactatt acaaaaaata
     3661 aaggagtttt taaaaatgaa agacacatta atgagtatac aaataattcc taaaacacca
     3721 aacaatgaca atgttatacc ttacgtagac gaggcgatta aaataattga cgaatctggt
     3781 ttgcatttta gagtaggtcc gttagaaacg acagtacaag gaaatatgaa tgaatgttta
     3841 attttaatac aatcattaaa tgaacgaatg gtggaacttg aatgtccaag tattattagc
     3901 caagttaagt tttatcatgt gccagatggc atcactattg aaactttaac tgaaaaatat
     3961 gatgaataac attaaaagtg aagtaaactg gatttgaatt ggcttgttag agatgacgta
     4021 taactttaac tgtttttgca ctttatagtt aaatttaata taattattaa atgatacggg
     4081 caaatagaaa ggattttgta aagtgaacga agaacaaaga aaagcaagtt ctgtagatgt
     4141 tttagctgag agagataaga aagcagaaaa agattatagt aaatattttg aacatgttta
     4201 tcagccgcct aatttaaaag aagcgaaaaa aagaggtaaa caagaagttc gttataatag
     4261 agatttccaa attgatgaaa aatatcgcgg tatggggaac gagcgtacat ttttaattaa
     4321 aacatatgga tgtcaaatga atgcacatga cactgaggtc attgctggta tacttgaagc
     4381 attaggctat caagcaacga ctgatattaa cactgcagat gttattttaa ttaatacatg
     4441 tgcgattaga gaaaatgccg agaacaaagt gtttagtgaa ataggtaatt tgaagcattt
     4501 gaaaaaagaa cgacctgata ttttaatcgg tgtttgtggt tgtatgtcac aagaagagtc
     4561 agtagtgaat aaaattttaa aatcgtatca aaatgtagat atgatatttg gtacacataa
     4621 tattcatcat ttaccagaaa ttttagaaga agcatactta tctaaagcaa tggttgttga
     4681 agtatggtct aaagaaggag acgttattga aaatcttcca aaagtccgtg aaggcaacat
     4741 taaagcatgg gtcaatatta tgtatggttg tgataagttt tgtacatatt gtattgttcc
     4801 atttacaaga ggtaaagaac gaagccgtag acctgaagac attatagatg aagtacgtga
     4861 acttgctcgt gaaggttaca aagaaataac gcttttaggt caaaatgtaa attcttatgg
     4921 taaagattta caggatatag aatatgactt aggagatctt ttacaagcaa tttctaaaat
     4981 agcgattcca agagttcgtt tcacaacaag tcatccttgg gactttacag atcacatgat
     5041 tgatgttatt tcagagggtg gtaatatcgt tccgcatatc cacttgccag ttcaatctgg
     5101 aaataatgca gtattaaaaa taatgggtag aaaatataca cgagaaagtt atttggattt
     5161 agtaaaacga atcaaagata gaattcctaa tgtagcatta actacagata ttattgtagg
     5221 gtatccaaat gaatcagagg aacaatttga agaaacttta actctgtatg atgaagttgg
     5281 ttttgaacat gcatatacgt acttgtattc acaacgtgat ggtacgcctg ctgctaaaat
     5341 gaaagataat gtacctttaa atgtcaaaaa ggaacgattg caacgtttga ataaaaaagt
     5401 tggtcattat tcacaaatag ctatgagtaa gtacgaagga caaactgtaa cagtactttg
     5461 tgaaggaagt agtaaaaaag atgatcaggt tcttgctggc tacactgata aaaataagct
     5521 agttaatttc aaagcgccta aagaaatgat tggtaaacta gtggaagtac gaatagatga
     5581 agctaaacag tattcattaa atggcagttt tataaaggaa gtagagccgg aaatggtgat
     5641 tcaataaatg tataataaag atgacgtgtt gaaccaagcg gataatattg caaataaaat
     5701 taaaaatttg gatactatca aaacatatca acaaattgaa gcacagattc atcagaacca
     5761 aacgataaag actaaaatgg atatgttaaa aaagcatcaa aaacaagcag taaactttca
     5821 aaattacggg aaacaaaatg cgctagaaca gtcggaacat accattcaga gtatagaagc
     5881 agaaataaat acattgccca tagttgaaca gtttcaaact tcacaatatg aagcgaatca
     5941 attattgaaa atgtttgtat caacaatgga aacacgttta aatgaccata ataaagccaa
     6001 gcatagtgat taattacaaa caaaaaggag aacaatcata tgaaatcaag aaaactggct
     6061 ataactgcac ttttaattgc aataaatgtt gtattaagca gtattatcat cattcctcta
     6121 ggaccagtta aggcagcacc agtacagcat tttgtaaatg tattaagtgc ggtcatagta
     6181 ggtccttggt atggattagc tcaagcgctt atatcatcaa ttttaagagt tctttttggt
     6241 actggtacag cttttgcatt tccgggtagt atgattggag ttttattggc tagtatgttt
     6301 tacatatatc gtaaacatat atttatggcc gcggtcggtg aagtacttgg aactggtgtc
     6361 atcggaagtt taatttgtat accattagca tatttccttg ggcttcaaga cttcttcatt
     6421 aaaccgttaa tgattacgtt catagtctca agtgctatcg gatctattat aagttatttc
     6481 ttattaatta ctctaaaaaa acgtggtatt cttcaaaggt ttataaaata attttatgat
     6541 atatgatttc aaacagagtc a
//
LOCUS       DAKTIO010000145         6544 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.147, whole genome shotgun sequence.
ACCESSION   DAKTIO010000145 DAKTIO010000000
VERSION     DAKTIO010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6544
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.147"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            197..1000
                     /locus_tag="O0293_002187"
     CDS             197..1000
                     /locus_tag="O0293_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCY0281058.1"
                     /translation="MLMDPSLILPYLWVLVVLVFLEGLLAADNAIVMAVMVKHLPPEQ
                     RKKALFYGLLGAFVFRFLALFLISIIANFWFIQAAGAVYLIYMSIKNLWQFFKHPEIE
                     SPEAGDDHHYDESGEEIKASNKSFWGTVLKIEFADIAFAIDSMLAALAIAVTLPKVGI
                     HFGGMDLGQFVVMFLGGMIGVILMRYAATWFVELLNKYPGLEGAAFAIVGWVGVKLVV
                     MVLAHPDIAVLPEHFPHGVLWQSIFWTVLIGLVIIGWLGSVVKNKKSHK"
     gene            1219..3543
                     /locus_tag="O0293_002188"
     CDS             1219..3543
                     /locus_tag="O0293_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCY0281059.1"
                     /translation="MEWTLVDIGKKHVIPKSQYRRKRREFFHNEDREENLNQHEDKQN
                     IDNTTSKKADKQIHKDSIDKHERFKNSLSSHLEQRNRDVNENKAEESKSNQDSKSAYN
                     RDHYLTDDVSKKQNSLDSVDQDTVKSKYYEQNSEATLSTKSTDKVESTEMRKLSSDKN
                     KVGHEEQHVLSKPSEHDKETRIDSESSRTDSDSSMQTEKIKKDSSDGNKSSNLKSEVI
                     SDKSNTVPKLSESDDEVNNQKPLTLPEEQKLKRQQSQNEQTKTYTYGDSEQNDKSNHE
                     NDLSHHIPSISDDKDNVMRENHIVDDNPDNDINTPSLSKTDDDRKLDEKIHVEDKHKQ
                     NADSSETVGYQSQSTASHRSTEKRNISINDHDKLNGQKTNTKTSANNNQKKATSKLNK
                     GRATNNNYSDILKKFWMMYWPKLVILMGIIILIVILNAIFNNVNKNDRMNDNNDADAQ
                     KYTTTMKNANNTVKSVVTVENETSKDSSLPKDKASQDEVGSGVVYKKSGDTLYIVTNA
                     HVVGDKENQKITFSNNKSVVGKVLGKDKWSDLAVVKATSSDSSVKEIAIGDSNNLVLG
                     EPILVVGNPLGVDFKGTVTEGIISGLNRNVPIDFDKDNKYDMLMKAFQIDASVNPGNS
                     GGAVVNREGKLIGVVAAKISMPNVENMSFAIPVNEVQKIVKDLETKGKIDYPDVGVKM
                     KNIASLNSFERQAVKLPGKVKNGVVVDQVDNNGLADQSGLKKGDVITELDGKLLEDDL
                     RFRQIIFSHKDDLKSITAKIYRDGKEKEINIKLK"
     gene            3560..4918
                     /locus_tag="O0293_002189"
     CDS             3560..4918
                     /locus_tag="O0293_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCY0281060.1"
                     /translation="MSIFSQFLKRSSPQQGIVLYYIVAIVIAFLLLNLPYVHKPGVEV
                     NPIDTLFVAVSGISVTGLSPISIVDTYSTFGQLIILVILNIGGIGVMAIGTMLWVVLG
                     KHIGIRERQLIMLDNNKNTMSGTVKLIIDIVKSIFVIELVGAMLLAFYFYRDNPDLKY
                     AIMQGVFVSISATTNGGLDITGKSLIPYAHDYFVQAIVIFLIILGSIGFPVLLEVKAY
                     IQNRVTNFRFSLFTKITTSTYLFLFIVGVLAILLFEHNHAFKGLSWHQSLFYSLFQSA
                     TTRSAGLQTIDVTTLSDPTNIIMGILMFIGSSPSSVGGGIRTTTFAILILFLINFSNN
                     ADKTSIKVYNREVHIMDIQRSFAVFTMATILTFLGMLIISATENGKLTFLQVFFEVMS
                     AFGTCGLSLGVTSDISDISKVVLMILMFIGRVGLISFIIMIAGRREPDKFHYPKERIQ
                     IG"
     gene            5057..>6544
                     /locus_tag="O0293_002190"
     CDS             5057..>6544
                     /locus_tag="O0293_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCY0281061.1"
                     /translation="MEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQVRAQN
                     HRVILLDSGGSLAGSLAAYYYAIVAPYKRHPMIKLMNRMHYDASGVSPSEFKFGLSFL
                     TRSIALARFPWLSANIEYNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSEM
                     EQDVSIEKTLVASKRWIRYIHEVEEPDFLIVIYHGGLNKISNSTKNKKASSNEAEKLM
                     EELGVIDLMITAHQHQTIVGQDHETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDS
                     KVIDLNEYEEDQELLDLTFYDRKAVAYWSQEIISDKGLMLSVNGLQDLVCQTHPFSQL
                     LHDAIHLAFDNDITCVHVPMNGEKGLSGQIRNEDLYHAYPYPDKPMDMTISGQNIKDI
                     LEYSYSHLDFVNEQLSLTIIDETLCTMWQGFNYEIDMNQEPGQRVMLDQIDLTKSYRV
                     TMTDYCYRNYKNYLKNAIIHESYDETMSTLIAEKLRDPNYRISCSD"
BASE COUNT         2347 a          864 c         1240 g         2093 t
ORIGIN      
        1 tgaataacct atggcatttt gtcagagggg agtaacttaa gaatcatgac cgtataaatg
       61 attcgacact ttatcgtcat tacgaagata tcttccggta aagtgggcaa tttaaattgc
      121 ttagtgagac ctttgctatt tatttagcat aggtcttttt gtttgtactt aacttattta
      181 tttaaaggag ttgtacatgt taatggatcc aagtttgatc ttaccttatt tatgggtact
      241 tgtcgtttta gtatttttag aaggcttatt agcagcagat aacgcgattg ttatggctgt
      301 aatggttaag cacttaccac ccgaacaacg taaaaaagct ttgttttacg gtttgttagg
      361 tgcatttgta tttagatttt tagcattatt cttaattagt attatcgcga acttttggtt
      421 tattcaagct gcaggagcgg tttacttaat ttatatgtca atcaaaaatc tgtggcagtt
      481 ctttaaacac ccagaaattg aaagtcctga agctggagat gatcatcatt atgatgaatc
      541 tggtgaagag attaaagcaa gtaacaaatc attctgggga actgtgttga aaatagaatt
      601 tgcagatatc gcatttgcca ttgattctat gcttgctgct ttagctattg ctgtaacact
      661 tcctaaagtt ggtattcact ttggtggtat ggacttaggt cagttcgtag tcatgttcct
      721 aggtggaatg attggtgtta ttctaatgcg ttatgcagca acatggtttg tagagctatt
      781 aaacaaatat ccaggacttg aaggtgcagc cttcgcgatc gttggttggg taggtgttaa
      841 attagttgtc atggtattag cgcacccaga catcgctgta ttgcctgagc acttcccaca
      901 tggcgtatta tggcaatcta ttttctggac agtactaatt ggattagtaa ttatcggttg
      961 gttaggttca gttgttaaaa ataaaaaatc gcataaataa ttgatgtgaa gcggacaatc
     1021 ttaatttagt ttaaggttgt cctttttcat ttaattgagt gatttatgaa aaatggattt
     1081 tgaagaatgt gaatcaaaag atgcgatata gtattaagaa aatgtgcctt ttatatttag
     1141 cattttttca atagaaatta tatagatttt aaagcaaatt aggtgttaat gtgtcataat
     1201 gataagtgat tttattgaat ggagtggaca ttagtggata ttggtaaaaa acatgtaatt
     1261 cctaaaagtc agtaccgacg taagcgtcgt gaattcttcc acaacgaaga cagagaagaa
     1321 aatttaaatc aacatgaaga taaacaaaat atagataata caacatcaaa aaaagcagat
     1381 aagcaaatac ataaagattc aattgataag cacgaacgtt ttaaaaatag tttatcatcg
     1441 catttagaac agagaaaccg tgatgttaat gagaataaag ctgaagaaag taaaagtaat
     1501 caggatagta agtcagcata taacagagat cattatttaa cagacgatgt atctaaaaaa
     1561 caaaattcat tagattcagt ggaccaagat acagtgaaat caaaatatta tgagcaaaat
     1621 tctgaagcga ctttatcaac taaatcaacc gataaagtag aatcaactga aatgagaaag
     1681 ctaagttcag ataaaaacaa agttggtcat gaagagcaac atgtactttc taaaccttca
     1741 gaacatgata aagagactag aattgattct gagtcttcaa gaactgattc agacagctcg
     1801 atgcagacag agaaaataaa aaaagacagt tcagatggaa ataaaagtag taatctgaaa
     1861 tctgaagtaa tatcagacaa atcaaataca gtaccaaaat tgtcggaatc tgatgatgaa
     1921 gtaaataatc agaagccatt aactttaccg gaagaacaga aattgaaaag acagcaaagt
     1981 caaaatgagc aaacaaaaac ctatacatat ggtgatagcg aacaaaatga caagtctaat
     2041 catgaaaatg atttaagtca tcatatacca tcgataagtg atgataaaga taacgtcatg
     2101 agagaaaatc atattgttga cgataatcct gataatgata tcaatacacc atcattatca
     2161 aaaacagatg acgatcgaaa acttgatgaa aaaattcatg ttgaagataa acataaacaa
     2221 aatgcagact cgtctgaaac ggtgggatat caaagtcagt caactgcatc tcatcgtagc
     2281 actgaaaaaa gaaatatttc tattaatgac catgataaat taaacggtca aaaaacaaat
     2341 acaaagacat cggcaaataa taatcaaaaa aaggctacat caaaattgaa caaagggcgc
     2401 gctacgaata ataattatag tgacattttg aaaaagtttt ggatgatgta ttggcctaaa
     2461 ttagttattc taatgggtat tattattcta attgttattt tgaatgccat ttttaataat
     2521 gtgaacaaaa atgatcgcat gaatgataat aatgatgcag atgctcaaaa atatacgaca
     2581 acgatgaaaa atgccaataa cacagttaaa tcggtcgtta cagttgaaaa tgaaacatca
     2641 aaagattcat cattacctaa agataaagca tctcaagacg aagtgggatc aggtgttgta
     2701 tataaaaaat ctggagatac gttatatatt gttacgaatg cacacgttgt cggtgataaa
     2761 gaaaatcaaa aaataacttt ctcgaataat aaaagtgttg ttgggaaagt gcttggtaaa
     2821 gataaatggt cagatttagc tgttgttaaa gcaacttctt cagacagttc agtgaaagag
     2881 atagctattg gagattcaaa taatttagtg ttaggagagc caatattagt cgtaggtaat
     2941 ccacttggtg tagactttaa aggcactgtg acagaaggta ttatttcagg tctgaacaga
     3001 aatgttccta ttgatttcga taaagataat aaatatgata tgttgatgaa agctttccaa
     3061 attgatgcat cagtaaatcc aggtaactcg ggtggtgctg tcgtcaatag agaaggaaaa
     3121 ttaataggtg tagttgcagc taaaattagt atgccaaacg ttgaaaatat gtcatttgca
     3181 atacctgtta atgaagtaca aaagattgta aaagatttag aaacaaaagg taaaattgac
     3241 tatcccgatg taggtgttaa aatgaagaat attgccagtc taaatagttt tgaaagacaa
     3301 gcagttaaat tgccaggaaa agttaagaac ggtgttgttg tagatcaagt tgacaacaat
     3361 ggtttagcag atcaatctgg tctgaaaaaa ggtgatgtaa ttactgaatt agatggcaaa
     3421 cttttagaag atgatttacg ctttaggcag attatattta gtcataaaga tgacttgaaa
     3481 tcaattacag cgaagattta tagagatggt aaagagaaag aaattaatat taaactaaaa
     3541 taatgttgag gtgaacaacg tgtcaatttt tagccagttt ttaaaaagat caagccctca
     3601 acaaggtatt gtattgtact atatcgtcgc aattgtcatt gcatttttat tattaaactt
     3661 accgtatgtt cataaaccag gtgtagaagt aaatccaatt gacacattat ttgttgccgt
     3721 atccggaatt agtgttactg gattgtctcc gataagtatt gtcgatacct attctacatt
     3781 tggacaatta attatcctcg tgatattaaa tattggtgga attggcgtca tggcaattgg
     3841 tacgatgtta tgggtggtac taggtaaaca tattggaatt agagaacgtc agttaattat
     3901 gttagataat aacaaaaaca caatgagtgg taccgtcaaa ttgattattg atattgtaaa
     3961 atcaatattt gtaatcgaac tcgtaggagc catgttatta gcattttact tttatcgaga
     4021 taatccagat ttaaaatatg caatcatgca aggtgttttt gtttctattt ctgccactac
     4081 caatggtgga ttagatatta caggtaagtc attaattcct tatgcacatg attattttgt
     4141 acaagcgata gttatatttt taataatttt aggatcaatc ggcttcccag tattattaga
     4201 agttaaagct tatattcaaa atagggttac taattttaga ttttcattat ttactaaaat
     4261 tacgacatca acatatttat tcctatttat tgttggggta ttagccattc tattatttga
     4321 acataaccat gcgttcaaag gtttaagttg gcatcaatcg ttattctatt cgctgtttca
     4381 atcagcgact acaagaagtg cgggtcttca aacaattgat gtgacaacac taagtgaccc
     4441 cactaatatt atcatgggta ttttaatgtt tataggatct tcgccaagtt cggttggtgg
     4501 cggtattcgt acaacaactt tcgctatttt aattttgttt ttaattaact ttagtaataa
     4561 tgccgataaa acatccatta aagtttacaa tagagaagta cacattatgg atattcaacg
     4621 ttcatttgca gtatttacaa tggcgacaat tttaacattt ttaggaatgc taattatatc
     4681 agctactgaa aatggtaagc ttacattttt acaagtattt tttgaagtca tgtctgcatt
     4741 tggaacttgt ggactatcgc ttggtgtcac aagtgatatt agtgatattt ctaaggtcgt
     4801 actaatgata ctcatgttta taggacgtgt tggcttaata tcatttatca ttatgatagc
     4861 aggacgtcga gaaccagata aattccatta tccaaaagaa cgtattcaaa taggataata
     4921 taatagcaat ctaagtttag ttaatgtaga ttttaactgg aacttagatt gcttttttag
     4981 tttgtatttt taacttattt tataagacga ttggtttcga aaatggtaaa ctagtaacaa
     5041 tgagaggtgt aacatgatgg aaaaaaatga aaacattaat gtagagattt taactacgtc
     5101 agatatgcat agtcatttct taaatggtga ttatggttca aatatttata gagctggtac
     5161 ttatgttaac caagtaagag cacaaaatca tcgcgtcatt ttattagata gtggcggaag
     5221 tttagctggc tcgttagcgg cctattatta tgctattgtt gcaccttata aacgacatcc
     5281 aatgataaag ttaatgaaca gaatgcatta tgatgctagc ggtgtgagtc caagtgaatt
     5341 caagtttggt ttatcatttt taactcgttc aattgctttg gcacgttttc catggttatc
     5401 agcaaatatt gaatacaatg ttactaagga gccttatttt tcaactccat attgtattaa
     5461 acattttggt gacttaaaaa ttgctatcgt aggcgtcaca gcagatggtt taatggaaaa
     5521 tgagtattct gaaatggagc aagatgtatc tattgaaaag acattagtgg catcaaaacg
     5581 ttggattaga tatatccatg aagttgaaga gccagatttt ttgattgtaa tttatcatgg
     5641 tggattgaat aaaattagta atagtacgaa aaataaaaag gcaagttcga atgaagctga
     5701 aaaattaatg gaagaactcg gtgttataga tttaatgatt acagctcatc agcatcaaac
     5761 aatagtaggt caagatcatg aaacgtatta tgttcaggct ggtcaagatg ccaaagagct
     5821 tgtacatctt tcgattaatt ttaaaaagag aacaacaact tatgatgttg aaagcattga
     5881 ttctaaagtg attgacttaa atgagtatga agaggatcaa gaattattag atttaacatt
     5941 ctatgataga aaagcagtgg cttattggtc acaggaaatc ataagtgata aaggtttgat
     6001 gttatcagta aatgggttac aagatttagt ctgtcaaaca catccatttt cgcaattatt
     6061 acatgatgca attcaccttg catttgataa tgatataaca tgtgtccacg tgcctatgaa
     6121 cggagagaag gggttgagtg gacagattcg aaatgaagat ttgtatcatg catacccata
     6181 tccagataag ccaatggata tgacaattag tggtcaaaat atcaaagata tattggagta
     6241 tagttattca catttagatt ttgttaacga gcaattaagc ttaacaatta ttgatgaaac
     6301 gttatgtaca atgtggcaag gattcaatta tgagattgat atgaatcaag aacctgggca
     6361 acgagtaatg ttagatcaaa ttgatttgac taagagttat agagttacaa tgactgacta
     6421 ttgttatcgt aactacaaga attatttaaa aaatgctatt atacatgaat catacgatga
     6481 aacaatgagt acattaattg cagagaagtt aagagatccg aattatcgta tttcatgtag
     6541 tgat
//
LOCUS       DAKTIO010000146         6504 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.148, whole genome shotgun sequence.
ACCESSION   DAKTIO010000146 DAKTIO010000000
VERSION     DAKTIO010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6504
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.148"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..1466
                     /locus_tag="O0293_002191"
     CDS             <1..1466
                     /locus_tag="O0293_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCY0281062.1"
                     /translation="SVLRELVKHPKFNQLDIDLYDDKVNMGQGVPFQNDSSELLINMP
                     SKKMSLNLDDETEFWKWYKQQTDFNFDEPAYLPRFVFGHYMKSYLSMFTKKYPNISTN
                     YNKVQEIYTNSNIDETNLTYYICTTNSGQSWQAYDYVFLTCGTFAYHDPYNLKGKKGY
                     IATPYPTYNTLDEVNELDDIAIIGTGLASLDVVRYVAAHHPKLPITMTSRSAHLPSVR
                     GTMIDVTFKYLTKDKLNDIKKHHFGNAPLDTLVSLFLKECADYDIDFKKLVHRRTGNH
                     IADLKYDLARPTEMGIFQSMIEHLKENLNWIWNSLSIEDQHQFNQKYSKMIQLNSNPM
                     PPRTAELIIELIENKSLILKKDLEDVKHDGKLYYFSYKNQESVDIYNVVINATGAKSH
                     LNELDQDDQLIRNLENRQIVQAHPMGGIQIIPETNQVISPRFGTLTNMIAIGQMTNGV
                     NKLRNGVKMIVEQVAHTVSQLYDALESNEQQQRSDNQ"
     regulatory      1685..1820
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1947..2990
                     /gene="ribD"
                     /locus_tag="O0293_002192"
     CDS             1947..2990
                     /gene="ribD"
                     /locus_tag="O0293_002192"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCY0281063.1"
                     /translation="MSQFMDYAIQLANMVQGQTGVNPPVGAVVVKEGRIVGIGAHLRK
                     GDKHAEVQALDMAQQNAEGATIYITLEPCSHFGSTPPCVNKIIDCKIAKVVYATKDNS
                     LDTHGDETLRAHGIEVECVDDERASQLYQDFFKAKAKQLPQITVKVSASLDGKQANDN
                     GQSQWITNKEVKQDVYKLRHRHDAVLTGRRTVELDDPQYTTRIQDGKNPIKVILSKSG
                     NIHFNQQIYQDESTPIWIYTENPNLTTNQTHIEIIYLKSCDLTTILHNLYKRGVGTLL
                     VEAGPTTTSEFLQSNYIDEFILYYAPKLIGGSGNYQFYQTNDVIEIPDANQFEIVHSE
                     LLNQNVKLTLRKK"
     gene            2997..3629
                     /locus_tag="O0293_002193"
     CDS             2997..3629
                     /locus_tag="O0293_002193"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCY0281064.1"
                     /translation="MFTGIVEEIGVVKSVQIRQSVRTIEIEAHKITADMHIGDSISVN
                     GACLTVIDFNQTSFTVQVIKGTENKTYLADVKRQSEVNLERAMSGNGRFGGHFVLGHV
                     DELGTVSKINETANAKIITIQCSQHINNQLVKQGSITVDGVSLTVFDKHDNSFDIHLI
                     PETRRSTILSSKKLGDKVHLETDVLFKYVENILNKDKDQLSVDKLRAFGF"
     gene            3640..4821
                     /gene="ribB"
                     /locus_tag="O0293_002194"
     CDS             3640..4821
                     /gene="ribB"
                     /locus_tag="O0293_002194"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCY0281065.1"
                     /translation="MQFDNIDSALMALKNGEPIIVVDDENRENEGDLVAVTEWMNDNT
                     INFMAKEARGLICAPVSKDIAQRLDLVQMVDDNSDIFGTQFTVSIDHVDTTTGISAYE
                     RTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEAAVDLAKLTGAKPAGV
                     ICEIMNDDGTMAKGQDLQKFKEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFG
                     TFDMYGFKATYTDEEIVVLTKGAIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMK
                     YINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLALGFDEDLRDYHIAAQ
                     ILKYFNIEHINLLSNNPSKFEGLKQYGIDIAERIEVIVPETVHNHDYMETKKIKMGHL
                     I"
     gene            4834..5298
                     /locus_tag="O0293_002195"
     CDS             4834..5298
                     /locus_tag="O0293_002195"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCY0281066.1"
                     /translation="MNFEGKLIGKDLKVAIVVSRFNDFITGRLLEGAKDTLIRHDVNE
                     DNIDVAFVPGAFEIPLVAKKLASSGNYDAVITLGCVIRGATSHYDYVCNEVAKGVSKV
                     NDQTNVPVIFGILTTESIEQAVERAGTKAGNKGAEAAVSAIEMANLLKSIKA"
     gene            complement(5420..6421)
                     /locus_tag="O0293_002196"
     CDS             complement(5420..6421)
                     /locus_tag="O0293_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase"
                     /protein_id="HCY0281067.1"
                     /translation="MALLKNFFIGLSNNSFLNNAAKKVGPRLGANKVVAGNTIPELIN
                     TIEYLNDKNIAVTVDNLGEFVGTVEESNHAKEQILTIMDALHQHGVKAHMSVKLSQLG
                     AEFDLELAYQNLREILLKANTYNNMHINIDTEKYASLQQIVQVLDRLKGEFRNVGTVI
                     QAYLYDSHELVDKYQDLRLRLVKGAYKENESIAFQSKEDVDANYIKIIEQRLLNARNF
                     TSIATHDHRIINHVKQFMKENHIEKDRMEFQMLYGFRSELAEEIANEGYNFTIYVPYG
                     DDWFAYFMRRLAERPQNLSLAVKEFVKPAGLKRVGIIAALGATVMLGLSTIKKLCRK"
BASE COUNT         2305 a          947 c         1200 g         2052 t
ORIGIN      
        1 taagtgtatt gagagagtta gttaagcacc cgaagtttaa tcaattagat atagatttat
       61 acgatgataa agtaaatatg ggacagggcg taccatttca aaatgatagt tctgagctgc
      121 taattaatat gccttcgaaa aagatgagtt taaatttaga tgatgagact gaattttgga
      181 agtggtataa acaacaaact gattttaatt ttgatgaacc tgcatattta ccgagatttg
      241 tattcggaca ttatatgaaa tcttatttat ctatgttcac gaaaaaatat ccaaatatat
      301 caactaatta taataaagtt caagagattt atacaaattc taatatcgat gaaacgaatt
      361 taacttatta catatgtact acaaattcag gacaatcatg gcaagcgtac gattatgtat
      421 ttttaacatg tggtacattt gcctaccatg atccatataa cttaaagggt aaaaaaggat
      481 atatcgctac gccttatcca acatataata cgttagacga agtaaatgaa ctggacgata
      541 ttgcgattat tggtacaggc cttgccagtt tagatgttgt ccgttatgtt gctgctcatc
      601 atccaaaatt accaattact atgacgagtc gttctgctca tttaccaagt gtaagaggta
      661 caatgataga cgtgacattc aagtacttaa ctaaagacaa attaaatgac attaaaaaac
      721 atcatttcgg caatgcaccg cttgatactt tagtttcttt atttttaaaa gaatgtgctg
      781 attatgacat tgattttaaa aaattagtac atagacgtac aggaaatcat attgcagatt
      841 tgaagtatga tttagcgcgt ccaacagaaa tgggcatttt ccaaagtatg attgaacatt
      901 tgaaagagaa tttaaattgg atatggaata gcttaagtat tgaagaccaa catcaattta
      961 atcaaaaata ctcaaagatg attcaattaa attcaaatcc aatgcctcca agaactgctg
     1021 aactaattat tgaattaata gaaaataagt ctttaatttt gaaaaaagat ttagaagatg
     1081 tgaaacatga tggcaagttg tattattttt catacaaaaa ccaagaatca gtagacatat
     1141 ataatgttgt cattaacgcg acaggtgcaa aaagtcattt aaatgaattg gatcaagatg
     1201 atcaattaat tagaaatctt gaaaatagac aaattgtaca agcacaccca atgggaggca
     1261 tacaaatcat tccggaaacc aatcaagtta ttagtccaag gtttggaaca ttaacaaata
     1321 tgattgcgat aggtcaaatg acaaacggtg ttaataaact acgaaacggt gtgaaaatga
     1381 ttgtagaaca agttgcgcat acagtatctc aattatatga tgctttagaa tcgaatgagc
     1441 aacaacagcg cagtgacaat caataatttg taactagaag ataataaaga gaacgctcta
     1501 tagagacgaa ttgaaggttt gattttaatg tctgttagta agaatcatat caatgagatg
     1561 cctatagtac tcagattata ttaaattaaa accgtcatta attgtttttt cagaaaacat
     1621 atagtatcat tttaaatgta gttgacatac tacgtactca aataatctat aacaatttca
     1681 tatataattc tttcggggca gggtgaaatt cccaaccggc agtaaataaa gcctgcgacc
     1741 tgctaatatg tttcatatta gtggctgatc tagtgagatt ctagagccga cagttaaagt
     1801 ctggatggga gaaagaatgt taattatcga caaagataat gtagcgtatt tgtaaaaatg
     1861 tgtacaaata ggcttattta acgataaatt tttctccttg catcttaatt catgatgtga
     1921 ggatttttgt ttatagaggt gatcatttga gtcaatttat ggattatgcg attcaacttg
     1981 caaatatggt acaaggtcaa acaggtgtta atccacccgt tggcgctgtt gtagttaaag
     2041 aaggtaggat tgttggtatt ggtgcacact tgagaaaagg tgacaagcat gcggaggttc
     2101 aagcacttga tatggcacaa caaaatgctg aaggtgcgac gatttatatt acgttagagc
     2161 catgtagtca ttttggttca acaccaccct gtgttaacaa aataattgat tgtaagatag
     2221 caaaagtagt atacgcaaca aaagacaatt cgttagacac acatggtgat gagacgttac
     2281 gggctcacgg tattgaggtt gaatgcgttg atgatgaacg ggcatcacaa ttataccaag
     2341 acttttttaa agcaaaagca aagcaactgc cacaaattac agtgaaagta tctgcaagtt
     2401 tagatggtaa acaagcgaat gataatggac aaagtcaatg gattactaac aaagaggtta
     2461 aacaagatgt ctataagtta agacatcgac acgacgcagt gttaactgga agacgtacag
     2521 ttgaattaga tgatccacaa tatactacac gtattcaaga tggaaaaaac cctataaaag
     2581 taatattgtc taagtctggg aatattcatt ttaatcagca aatttatcaa gatgaatcaa
     2641 caccaatttg gatatatact gaaaatccaa atttaacaac caatcaaaca catattgaaa
     2701 ttatttactt gaagtcttgc gatttaacaa caattcttca caatttatat aaaagaggag
     2761 ttggaacttt gctagttgag gcaggtccaa ccactacttc agaatttctc caatctaatt
     2821 atatagatga atttattctc tattatgccc cgaaattaat tggcggatct ggaaattatc
     2881 aattttatca aacaaatgat gtgattgaga taccagatgc gaaccaattt gaaattgttc
     2941 attccgagtt attaaatcaa aatgttaaat taactttacg aaagaagtga tgatgcatgt
     3001 ttactggcat cgttgaagaa ataggtgtcg ttaaaagtgt tcaaattcgt caatcagtca
     3061 ggacgattga aattgaagca cataagatta cggcagatat gcatattggt gattccatca
     3121 gtgttaatgg tgcatgttta acagtgattg attttaatca gacatctttt actgttcaag
     3181 taattaaagg cactgaaaat aaaacctatt tagcagatgt taagcgacaa tcagaagtaa
     3241 atttagagcg tgccatgagt ggtaacggta ggtttggtgg acattttgtc ctcggtcatg
     3301 tagatgaact aggaacagtt tcaaaaataa atgaaacagc caatgcaaaa attattacga
     3361 ttcaatgtag ccaacatatt aataatcagt tagttaagca agggtctatt actgtggatg
     3421 gtgttagtct aacggtattt gataagcatg ataacagttt tgacattcat cttattccag
     3481 aaacgaggcg ttcaacgatt ttatcatcca aaaaattagg agataaagta catttagaaa
     3541 cagacgtttt gtttaaatat gttgaaaata ttttaaataa agataaagac caattatctg
     3601 tagataaatt aagagcattt ggtttttagg aggggtagca tgcaattcga taatattgac
     3661 agtgctttaa tggctttaaa aaatggagaa ccaattattg tagtagatga tgagaatcgt
     3721 gaaaatgaag gtgatttagt agcggttact gaatggatga acgataatac cattaatttt
     3781 atggcgaaag aagcaagggg attaatatgc gcaccagtgt ctaaagatat tgcacaacgt
     3841 ttggatttgg tacaaatggt tgatgataac tccgacatct ttggtacgca atttacagtg
     3901 agtattgatc atgtagatac aacaacagga attagtgctt atgaacgtac attgactgcc
     3961 aaaaagctca ttgatcctag tagtgaagct aaagatttta atcgtcctgg tcatttattt
     4021 ccattagtag cacaagataa aggcgtatta gctagaaatg gacacacaga agcggctgtt
     4081 gatttagcta aacttactgg tgccaagccc gctggtgtca tttgtgagat tatgaatgat
     4141 gacggcacga tggcgaaagg acaagattta caaaagttta aagaaaaaca tcaattaaag
     4201 atgattacga ttgatgattt aattgaatat cgtaaaaaat tagaaccaga aattgaattt
     4261 aaggcaaaag tgaaaatgcc tacagatttc ggaacatttg atatgtatgg ttttaaagcg
     4321 acatacacag atgaagagat agttgtactg acaaaaggtg caattcgaca acatgaaaat
     4381 gtacgcttac attctgcgtg ccttacaggc gatattttcc atagtcaacg ttgtgattgt
     4441 ggtgctcaac ttgaatcgtc tatgaagtat atcaatgaac atggtggcat gattatttat
     4501 ctacctcaag aaggtcgtgg cataggattg ttaaacaaat tacgcgcata tgaattaatt
     4561 gagcaaggat atgatacagt aactgcaaat ttagcattag gttttgatga agatttgcga
     4621 gattatcata ttgctgcaca gattttaaaa tattttaaca tcgaacatat caatttatta
     4681 agtaataatc caagtaaatt tgagggatta aaacaatatg gcattgatat tgcagaaaga
     4741 attgaagtta tcgtaccaga aacggtacat aatcatgatt atatggaaac gaaaaaaata
     4801 aaaatgggtc atttaatata ggaggatttt aacatgaatt ttgaaggtaa attaattgga
     4861 aaagatttga aagttgcaat cgtagttagt cgatttaatg attttatcac tggaagatta
     4921 cttgaaggtg caaaagatac tttgattcga catgatgtta atgaagacaa tattgatgta
     4981 gcatttgtgc caggtgcgtt tgaaattcct ttagtagcta aaaaattagc ctcatcagga
     5041 aattatgatg cagtaattac attaggatgc gtaattcgcg gtgctacgtc tcattatgat
     5101 tatgtttgta atgaagtcgc gaaaggtgtt tctaaagtga atgatcaaac taatgtacca
     5161 gtcatatttg gtattttaac gactgaaagt attgaacaag ctgtggaaag agcaggtacg
     5221 aaagctggca ataaaggtgc tgaagcagca gtaagtgcaa ttgaaatggc taatttatta
     5281 aaatctataa aagcatagag cactgttgtg agttcataat caaagattta taaatgtttc
     5341 agaactgcca atataacatt taggagccta gaacattgat tattgttcca gactcctaaa
     5401 ttattgttta tgtcttgctc tatttacggc ataatttttt aattgtactt aaacctaaca
     5461 taactgtagc tcctaaagct gcaattatgc caacacgttt taagccagca ggtttcacaa
     5521 attcttttac agcaagagat aggttttgtg ggcgttctgc taatcttctc ataaaatacg
     5581 caaaccaatc atcgccataa ggtacataaa tagtgaaatt atagccttca tttgcgattt
     5641 cttctgctaa ctctgatcta aaaccataga gcatttggaa ttccatacga tctttttcaa
     5701 tgtgattttc tttcataaat tgttttacat gattaatgat gcgatggtca tgtgttgcaa
     5761 ttgaagtgaa attgcgtgcg tttaacaaac gttgttcaat tattttgatg taatttgcat
     5821 ctacgtcttc cttagattga aatgcaattg attcgttttc tttatatgca cctttaacca
     5881 aacgtaatcg taaatcttgg tacttatcaa ctaattcgtg gctatcgtat aaatatgctt
     5941 gaattacagt accaacattt ctaaattcgc cttttaagcg atctaaaact tgaacaattt
     6001 gttgcaggct agcatatttt tcagtatcaa tatttatatg catattgttg taagtatttg
     6061 ctttaagtaa aatctctctt aaattttggt aagctaattc taagtcgaat tctgcaccta
     6121 actgactcaa tttaacagac atatgtgcct ttacgccatg ttgatgaagc gcgtccataa
     6181 ttgttaaaat ttgttcttta gcatgattac tttcttcaac tgtaccgaca aattccccta
     6241 aattgtctac cgtaacagcg atattcttgt catttaagta ttcgattgta ttaattaact
     6301 ctggaattgt atttccggca acgactttat tggcgcccaa acgtgggccc actttttttg
     6361 ctgcgttgtt taaaaaacta ttattagata atccgataaa aaaattcttt aatagtgcca
     6421 ttgctactcc tccaaaccct tgtgtttaca ataagtttag catgaaaaaa atattcgaga
     6481 aagcgttttc atcaaaaaca atca
//
LOCUS       DAKTIO010000147         6444 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.149, whole genome shotgun sequence.
ACCESSION   DAKTIO010000147 DAKTIO010000000
VERSION     DAKTIO010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6444
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.149"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(497..952)
                     /locus_tag="O0293_002197"
     CDS             complement(497..952)
                     /locus_tag="O0293_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HCY0281068.1"
                     /translation="MNNDKLQRMVENLSEEKFGRTFRHCAYFNKRLRTTGGRYLLKSH
                     DIEINPKQYEHYGEDAVVKIILHELCHYHLHIAGKGYQHKDQDFKRLSQQVGAPRFCN
                     SIESYQQRANYEYYCTKCHAKYIRIRKVDTNRMRCGHCNGKLRMKRQLK"
     gene            complement(945..3095)
                     /locus_tag="O0293_002198"
     CDS             complement(945..3095)
                     /locus_tag="O0293_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCY0281069.1"
                     /translation="MDNQLINSIIEKYQFSKKQIEAVLTLLEEKNTVPFIARYRKEQT
                     GGLDEVQIKQIDDEYQYMVNLQKRKEEVIKNIEQQGLLTEELKKDILKQNKLQRVEDL
                     YRPFKQKKKTRATEAKRKGLEPLAIWMKARKHEVSIEEKAQQFINEEVQSVEDAIKGA
                     QDIIAEQISDNPKYRTKILKDMYHQGVLTTSKKKNAEDEKGIFEMYYAYSEPIKRIAN
                     HRVLAVNRGEKEKVLSVKFEFDTTSVEDFIARQEINHNNVNRSYILEAIKDSLKRLIV
                     PSIEREIHADLTEKAENHAIDVFSENLRNLLLQPPMKGKQILGVDPAFRTGCKLAVIN
                     PFGTFIAKGVIYPHPPVSKKEAAEKDFVQMVKAYDVQLIAIGNGTASRETEQFVADLI
                     KKHQLPVQFIIVNEAGASVYSASEIARDEFPDFQVEERSAVSIGRRVQDPLSELVKID
                     PKSIGVGQYQHDVNQKALENALTFVVETAVNQVGVDVNTASSSLLQYVSGLSSQIAKN
                     IIAYREENGAIKHNKELSKIKRLGAKTFEQSIGFLRIVDGSEPLDNTSIHPESYKVTY
                     QLLDKLGFGGNDLGSDALKAKLNSLDMDELAIELQVGVPTLEDIIKSLKAPNRDPRDE
                     FETPILKSDVLSIEDLQEGMKLSGTVRNVVDFGAFVDIGVKQDGLVHVSKLSKKFVKN
                     PMDIVSVGDIVDVWIYSIDKNKDKVSLTMIDPHE"
     gene            complement(3529..4299)
                     /gene="sigB"
                     /locus_tag="O0293_002199"
     CDS             complement(3529..4299)
                     /gene="sigB"
                     /locus_tag="O0293_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigB"
                     /protein_id="HCY0281070.1"
                     /translation="MAKESKSANEISPEQINQWIKEHQENKNTDAQDKLVKHYQKLIE
                     SLAYKYSKGQSHHEDLVQVGMVGLIGAINRFDMSFERKFEAFLVPTVIGEIKRYLRDK
                     TWSVHVPRRIKEIGPRIKKVSDELTAELERSPSISEIADRLEVSEEEVLEAMEMGQSY
                     NALSVDHSIEADKDGSTVTLLDIMGQQDDHYDLTEKRMILEKILPILSDREREIIQCT
                     FIEGLSQKETGERIGLSQMHVSRLQRTAIKKLQEAAHQ"
     gene            complement(4274..4753)
                     /gene="rsbW"
                     /locus_tag="O0293_002200"
     CDS             complement(4274..4753)
                     /gene="rsbW"
                     /locus_tag="O0293_002200"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma B factor RsbW"
                     /protein_id="HCY0281071.1"
                     /translation="MQSKEDFIEMRVPASAEYVSLIRLTLSGVFSRAGATYDDIEDAK
                     IAVSEAVTNAVKHAYKENNNVGIINIYFEILEDKIKIVISDKGDSFDYETTKSKIGPY
                     DKDENIDFLREGGLGLFLIESLMDEVTVYKESGVTISMTKYIKKEQVRNNGERVEIS"
     gene            complement(4755..5081)
                     /locus_tag="O0293_002201"
     CDS             complement(4755..5081)
                     /locus_tag="O0293_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HCY0281072.1"
                     /translation="MNLNIETTTQDKFYEVKVGGELDVYTVPELEEVLTPMRQDGTRD
                     IYVNLENVSYMDSTGLGLFVGTLKALNQNDKELYILGVSDRIGRLFEITGLKDLMHVN
                     EGTEVE"
     gene            complement(5200..6201)
                     /locus_tag="O0293_002202"
     CDS             complement(5200..6201)
                     /locus_tag="O0293_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HCY0281073.1"
                     /translation="MEEFKQHYKGLIDESLTCQDKVELIKKCEKYTDEVIRKDVLPED
                     IVDIHKNYILTLNLTREDVFKTLDVLQEIVKGFGYSYRDYQRLVDKLQVHDKEIDLAS
                     SLQQTMLKTDIPQFDSIQIGVISVAAQKVSGDYFNLIDHNDGTMSFAVADVIGKGIPA
                     ALAMSMIKFGMDSYGHSQLPSDGLKRLNRVVEKNINQNMFVTMFYGLYEEMNHLLYCS
                     SAGHEPGYIYRAEKEEFEEISVRGRVLGISSQTRYQQQEIPIYLDDLIIILTDGVTEA
                     RNSEGTFIDKQKLLEYIKKHKHMHPQDIVQIIYEAILKLQNPNKKDDMTILIIKRVN"
BASE COUNT         1963 a         1225 c          796 g         2460 t
ORIGIN      
        1 ttagaactta ttgtcaacat attttataaa ttaaatttag aattgactgt ctttcaaatc
       61 aaagtttatt taatttgttc ttttttaagg aacgtgttta atcatactaa ctataagctg
      121 tacagtcaat agaaaagttc aatcaaaatg tcatgaaaaa tgtataatta tcttttgtgc
      181 tactttaaag ctttaattgg gtaaagttta tatattaatt attactaaaa tacgccaaga
      241 tttttaatct aatcatttta atttcatcct atcaagccac aatttatgcc attacctata
      301 tcataagtag aaaagccgag attgcgaagt attaaattac gacaatctcg gctttaaaca
      361 ttttataaac acaatttctt cttattcaat tactatgttt accaatcaaa ataactcact
      421 gataaattct atataaattc catacaactc ataaataatc ttacatatac acgaattagc
      481 tctatttgta ttgtacttat ttcaattgtc ttttcattct taattttcca ttgcaatgtc
      541 cgcaacgcat acgattcgta tcaactttac ggatacgtat atattttgca tgacatttag
      601 tgcaataata ttcataattt gcccgttgtt gatagctttc aatgctgtta caaaatctag
      661 gcgctccaac ctgttggctc aatcgtttaa aatcttgatc tttatgttga taacctttac
      721 cagcaatatg caagtgataa tgacacaatt cgtgcagtat aatttttaca acagcatctt
      781 ctccataatg ctcatattgt tttggattaa tttcaatatc atgggacttt aaaagataac
      841 gtccgcctgt tgtacgtaac cttttattaa aatatgcaca atgtcgaaac gtacgtccaa
      901 atttttcttc cgaaagattc tcaaccattc gctgaagttt gtcattattc atgtggatca
      961 atcatcgtta atgatacttt gtctttattt ttgtcaatac tgtaaatcca aacgtcaacg
     1021 atatcaccaa cactgacaat atccattgga ttttttacga acttcttaga aagtttcgaa
     1081 acatggacaa gtccatcttg tttaacacct atatctacaa atgctccaaa atctactaca
     1141 tttctaactg taccactcaa tttcattcct tcttgtaaat cttcaattga tagcacatcg
     1201 gatttaagga ttggtgtttc aaactcgtcc cttggatctc gattaggtgc tttcaaggat
     1261 ttaataatat cctctaatgt aggtacaccg acttgtaatt caatcgccag ttcatccata
     1321 tccaatgaat ttaatttagc ttttaatgca tcactaccta aatcattgcc accaaatccg
     1381 agtttatcta ataactgata cgttacttta tagctttcgg gatgaatcga tgtattatcc
     1441 aatggctctg aaccgtctac tattcttaaa aatcctatac tctgctcaaa agttttagca
     1501 cccaaacgct taatcttgct taactcttta ttatgcttta ttgcgccatt ttcttctcta
     1561 tatgcaatta tatttttagc aatttgcgaa cttagaccag acacatattg taataatgat
     1621 gatgatgctg tattgacatc aacacctact tggttaactg ctgtttcaac aacgaaagtt
     1681 aatgcatttt ctagtgcttt ttgattaaca tcatgctggt attgtccgac accaatagat
     1741 ttaggatcga ttttaactaa ttcacttaat ggatcttgaa cgcgtcgtcc aattgatacg
     1801 gcacttcttt cttccacttg aaaatcagga aattcatctc tagcaatttc tgatgctgag
     1861 tatactgaag cgcccgcttc attgacaatg atgaattgta ctggcaactg atgcttttta
     1921 attaaatctg caacaaattg ttctgtttca cgacttgcag taccattgcc aattgcaatt
     1981 aattgcacat catacgcttt aaccatttgt acaaaatcct tctctgctgc ctctttttta
     2041 gaaactggtg gatgcggata aatcacacct tttgctataa aagtaccgaa tgggttaatg
     2101 actgctaatt tacaacctgt tctaaatgct ggatctacgc ctaatatttg tttacctttc
     2161 attggaggtt gcagtaatag atttcttaag ttttcactaa aaacatctat tgcatgattt
     2221 tcagcttttt cagttaaatc agcatggatt tcacgctcta tcgaagggac aattaagcgt
     2281 ttcaagctat ctttaatcgc ctctaaaata taactgcgat ttacattatt atgattgatt
     2341 tcttgacgtg caatgaaatc ctctactgat gtcgtatcga attcaaactt tacagataat
     2401 actttctctt tttcaccacg attaacagct aaaactctat gattagcaat gcgtttaatt
     2461 ggctcactat atgcatagta catttcaaaa ataccttttt catcttcagc atttttcttt
     2521 ttagatgtag ttaacacacc ttgatgatac atatctttta aaatttttgt tctatattta
     2581 ggattatctg aaatttgttc cgcaataata tcttgtgcac ctttgatagc atcttcaacc
     2641 gattgcactt cttcatttat aaattgttgt gctttttctt caattgagac ttcatgttta
     2701 cgtgccttca tccatatcgc taatggctct aaccctttac gtttcgcctc agttgccctt
     2761 gtctttttct tttgtttaaa aggcctatat aggtcttcaa cacgttgtaa tttgttctgt
     2821 tttaaaatat ccttctttaa ttcctcagta agtaatcctt gctgttctat atttttgata
     2881 acttcttctt tacgtttttg taaattgacc atatattggt attcgtcatc aatttgcttt
     2941 atttgaactt catctagtcc accagtttgc tcttttcgat acctcgcaat aaatggtact
     3001 gtattttttt cttctagcag tgttaatact gcttcaattt gttttttact aaattgatat
     3061 ttctctatga ttgaattaat caattgattg tccatttact taacccacac tttttcttaa
     3121 atttactcat ttattttaac atattctaaa atacttctat taaggtatga tacttaatgt
     3181 aattttcact tccaaaacat ttaaaacgaa tgattaggtc atactatatt ttaaaacgca
     3241 ctaagattat tgaaaatgac acaccatcat ttctaaattt cataatctaa gactactttt
     3301 tttattctaa tagcaaccca cctatttaaa acgacaatta gatatcttct tcacttaaat
     3361 tattaatact atatcgcgac atttatgtgg atacacatta aactacacta ataatgtatt
     3421 aattcgaaaa tcttaattaa attaaaaata cccctcgatt tcaatttaat aatcaaggga
     3481 taattatatg gatttttcat tataaaacgt atcattaata aacaaattct attgatgtgc
     3541 tgcttcttgt aatttcttaa ttgccgttct ctgaagtcgt gatacatgca tttgacttaa
     3601 accgatacgc tcacctgtct ctttttgact caatccttca ataaacgtac attgtatgat
     3661 ttctcgttcg cgatcagata atataggtaa tattttttct aaaatcatac gtttttcagt
     3721 taagtcataa tggtcatctt gttgccccat aatatctaat agcgtaacag ttgaaccatc
     3781 tttatcagct tcaatggaat gatcaacact taacgcatta taactttgtc ccatttccat
     3841 tgcttctaac acttcttctt ctgagacttc taatcgatca gctatttcac tgatagaagg
     3901 tgaacgctct aattcagcgg ttagttcatc gctcactttt ttgattcttg gcccaatttc
     3961 tttaatacgt ctcggaacat gtacactcca agttttatct cgtagatatc ttttgatttc
     4021 accgattaca gtaggtacta aaaaggcttc aaacttccgt tcaaaggaca tatcgaatct
     4081 atttatggca cctattaaac caaccatacc aacttgaact aaatcttcgt gatgtgattg
     4141 tcctttagaa tatttatatg ccaatgactc aattagtttt tggtaatgtt taactaactt
     4201 atcctgtgca tctgtattct tattttcttg gtgttcttta atccattggt taatttgctc
     4261 aggtgaaatt tcattagctg atttcgactc tttcgccatt atttcgcacc tgctcttttt
     4321 ttatatactt agtcatactg attgtcacac cagattcttt atatactgtg acttcatcca
     4381 ttaaagattc gattaaaaat aaacctaggc caccttcgcg taaaaagtct atattttcgt
     4441 ctttatcgta aggacctatt tttgatttag ttgtttcata atcaaaactg tcacctttat
     4501 cagaaataac aattttaatt ttatcttcta aaatttcaaa atatatgtta ataatgccca
     4561 cattgttatt ttctttgtat gcatgtttaa ctgcatttgt cacagcttca ctaactgcaa
     4621 tcttggcatc ttcaatatca tcatatgtag caccagctct cgaaaaaacg ccagaaagtg
     4681 ttaaacgaat taaacttaca tactctgccg atgctggcac gcgcatttcg ataaaatctt
     4741 ctttagattg catgttattc gacctccgtt ccttcattaa catgcattaa atccttaaga
     4801 ccagtaattt caaatagtct accgatacga tctgacacac ctaaaatgta tagttcttta
     4861 tcattttggt ttaatgcttt taatgtacct acgaataaac ctaaacctgt cgaatccata
     4921 taactcacat tttctaaatt aacataaata tcacgagttc catcttgtct cataggtgtt
     4981 aaaacctctt ctaattcagg cacagtataa acatctaatt ctccaccgac tttaacttcg
     5041 taaaatttat cttgagtggt tgtttctata ttaagattca tttcattaca ctcctactta
     5101 ttaaaaatat ttatcttcat tttaagcgct gtatccacca tatttatatt tcaaattatt
     5161 atatacccat cgaaataatt tctaatcttc ttttttaaat taatttactc tttttataat
     5221 caaaatagtc atatcatctt ttttatttgg gttttgaagc tttaaaattg cttcatagat
     5281 aatttgaaca atatcttgtg ggtgcatatg tttatgtttt ttaatatatt ctaaaagttt
     5341 ttgtttatct ataaaggtac cttcactatt tctagcttca gtcacaccat ccgttaaaat
     5401 gataattaaa tcatcaaggt atattggaat ttcttgttgt tgatatcgtg tttgtgaact
     5461 gattcctaac actctacctc taactgaaat ttcttcaaat tcttcttttt cagcgcgata
     5521 aatatatcca ggctcatgac cagctgaact acaatacaat aaatggttca tttcttcata
     5581 taaaccataa aacattgtga cgaacatatt ttgattaata ttcttttcaa caacacgatt
     5641 taaacgtttt aaaccatcac tcggtaattg tgagtgtcca taagaatcca tgccaaactt
     5701 tatcatactc attgctaaag cagctggtat accttttcca atgacatctg caacagcaaa
     5761 gctcattgtg ccatcgttat ggtcaattaa attaaaataa tctccactta ctttttgtgc
     5821 cgccactgaa ataacgccaa tttgaatact atcaaattgt ggaatatctg ttttaagcat
     5881 tgtttgttgt aagctagaag ctaagtctat ctctttatcg tgaacttgaa gtttatctac
     5941 caatctttga taatctcgat aactataacc aaagccttta acgatttctt gtaagacatc
     6001 taatgtcttg aacacatctt cacgcgttaa gtttaacgtc aatatatagt ttttgtgaat
     6061 atcgacaatg tcttcaggca agacgtcctt acgaatcact tcgtcagtgt atttctcaca
     6121 cttttttatc aattctactt tatcttggca cgttaaactt tcatcaatta aacccttata
     6181 atgttgctta aattcttcca cgattagttg cctcctcact tcccttacaa agcaaatgct
     6241 taagcaagga taattatgat tcacttacta acatttattt aattgcactt atcaattttg
     6301 tcgtttgcaa ttttgctgta gatgatactt ttaagctaaa aaaaacttta ggatggaata
     6361 cctaaagcct aaaaaactta agacatcctt ttataataaa aaatatgaat atcataactg
     6421 gtatttgcta aaatctttgt ttat
//
LOCUS       DAKTIO010000148         6366 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.150, whole genome shotgun sequence.
ACCESSION   DAKTIO010000148 DAKTIO010000000
VERSION     DAKTIO010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6366
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.150"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            17..652
                     /locus_tag="O0293_002203"
     CDS             17..652
                     /locus_tag="O0293_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0281074.1"
                     /translation="MKLNNYSLKVKNKQLVDNCDLNFYLGQINHIVGKNGVGKSLLAK
                     DFLLNNSGNIPKSISQNVTLISSSSNIPNDITKDFLLSLLKSKFENNRQTFDKIYNIL
                     NIEAIPSNVLLKNLSDGQKQKLKLLSFLLEDHDLIILDEVTNALDKKTVNEIYEFLND
                     FIQSHQTKTIINITHNLSDLSALPGKYFIFKDLQIEEYQSKEEVINDYINL"
     gene            658..1389
                     /locus_tag="O0293_002204"
     CDS             658..1389
                     /locus_tag="O0293_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HCY0281075.1"
                     /translation="MSTLKLEFKKSISNKIIYTLVVLFTFLFLLGYFLPIGIDKVKSL
                     SYSQFFFSSYTVATQLGFLLFSFVIAYFINKEYSNKNILFYKLIGDNIFTFFYKKVAV
                     LFFECLVFIILSITIISIIYSDFSHYLLLIILFSLVILQYILVVGTISMVSPNILISL
                     GISIVYWIGSVILVAINKNIFGIVAPFEASNTMYRAVEKILNNESAFMCPTEIINIVS
                     FFVLLFIVNTIVLLLSRKRWLKIGM"
     gene            complement(1549..2169)
                     /gene="srtA"
                     /locus_tag="O0293_002205"
     CDS             complement(1549..2169)
                     /gene="srtA"
                     /locus_tag="O0293_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase SrtA"
                     /protein_id="HCY0281076.1"
                     /translation="MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQ
                     YDKNVKEQASKDKKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATPEQLNRGVS
                     FAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRD
                     VKPTDVGVLDEQKGKDKQLTLITCDDYNEKTGVWEKRKIFVATEVK"
     gene            2363..2854
                     /locus_tag="O0293_002206"
     CDS             2363..2854
                     /locus_tag="O0293_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCY0281077.1"
                     /translation="MIRYAKKEDLNAILAIYNDAIINTTAVYTYEPQTIDERVAWFET
                     KQRKHEPIFVFEENGSVLGFATFGSFRPWPAYLYTIEHSIYVDASARGKGIASQLLHH
                     LIVEAKAKGYRALVAGIDASNKASIQLHQKFAFKHAGTLTNVGFKFNRWLDLAFYELD
                     LQD"
     gene            complement(3292..3462)
                     /locus_tag="O0293_002207"
     CDS             complement(3292..3462)
                     /locus_tag="O0293_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281078.1"
                     /translation="MLRLSIIFIAFIINTTITYGYTTEGTWVNLLFKSLSLSMIIVFM
                     FYYIRFVIEKKR"
     gene            complement(3524..3622)
                     /locus_tag="O0293_002208"
     CDS             complement(3524..3622)
                     /locus_tag="O0293_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281079.1"
                     /translation="MIKKKHLSGSLVPDRCFLIISEEVERFMENAY"
     gene            complement(3641..4540)
                     /gene="sdaAA"
                     /locus_tag="O0293_002209"
     CDS             complement(3641..4540)
                     /gene="sdaAA"
                     /locus_tag="O0293_002209"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HCY0281080.1"
                     /translation="MFDSIRETIDYAVENNMSFADIMVKEEMELSGKSRDEVRAQMKQ
                     NLDVMRDAVIKGTTGDGVESVTGYTGHDAAKLRDYNETHHALSGYEMIDAVKGAIATN
                     EVNAAMGIICATPTAGSSGTIPGALFKLEKTHDLTEEQMIDFLFTSALFGRVVANNAS
                     VAGATGGCQAEVGSASAMAAAAAVAIFGGSPEASGHAMALAISNLLGLVCDPVAGLVE
                     IPCVMRNAIGSGNALISADLALAGIESRIPVDEVIEAMDKVGRNLPASLRETGLGGLA
                     GTPTGEAIKRKIFGTAEDMVKNN"
     gene            complement(4554..5234)
                     /gene="sdaAB"
                     /locus_tag="O0293_002210"
     CDS             complement(4554..5234)
                     /gene="sdaAB"
                     /locus_tag="O0293_002210"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HCY0281081.1"
                     /translation="MAKSYDYQSAFDIIGPVMMGPSSSHTAGAVKIGNSARAILGDIP
                     KNIEIHYYESFAQTHQGHGTDVAIVGGAMGYSTFDNRIKSSLEIAADDGIKIDIIEEE
                     GDSIGQHPNCAYIKSSRNDGRYIEIIGISIGGGTIKIKGIHINGLEVDLNHGLPILVV
                     DGNMTKAQVNHFINDINDMKLDCKDEFIKIDEDKCLVVIPLNKAISESTLNTIKEKYS
                     DVNVSYIN"
     gene            complement(5237..6280)
                     /locus_tag="O0293_002211"
     CDS             complement(5237..6280)
                     /locus_tag="O0293_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCY0281082.1"
                     /translation="MDILLGVGTLVLVLVIMTLFLKYAPYGKQGLQALSGAACATFLP
                     QAFLSYAIGGVFDIKFLQDIGDLAGSLSGIAVGILTCLNLGVAPVFAVIVGLVLHDFK
                     LLPAFIAAYGVAFLIKWIEKKVPEGLDLIVVILFAPAIAFGLASIITPGVLATLKQIG
                     SAVTAVGDNNPYALAVILGLVIPVTGMTPLSSMVLTSLLGLTGVPMAIGALTCTGSSF
                     VNLILFRKLNIGGPSKAFAVCIEPLTQIDLIAQYPVQLYGTNALIGVVNACIVTFSGL
                     VIGVKGMATPIAGAIVLFGFNNAVTSIVTIATVIIVSIVLAYIIGTLINKFNLMNINF
                     KMPSKKNHIKESV"
BASE COUNT         2017 a         1192 c          944 g         2213 t
ORIGIN      
        1 cataggagtt gtatacatga aactaaataa ctattcttta aaagttaaaa acaaacaact
       61 agttgacaat tgtgatttaa atttctatct tggtcagatc aatcacattg ttggtaaaaa
      121 tggtgtagga aaatctttat tagctaaaga tttcttacta aataatagtg gaaatatccc
      181 taagtccatt tctcaaaatg taaccttaat atcaagttca tcaaatattc ctaatgatat
      241 aacaaaagat tttttattat cattgttaaa atcaaaattt gaaaacaatc gacaaacatt
      301 cgataagatt tataacatac taaacatcga agcaataccg tctaacgtac tactaaaaaa
      361 cttgagtgat ggtcaaaaac aaaagcttaa attattaagt ttcttattgg aagatcatga
      421 tttaatcata ttagatgaag ttacaaacgc tttagacaag aaaacagtta atgaaattta
      481 tgaattttta aatgatttta ttcaaagcca tcaaactaaa actattatca atattacaca
      541 taatttatcc gatttaagtg ctttgccggg taaatacttt atttttaaag accttcaaat
      601 agaagagtac caatcaaaag aagaagtcat aaatgattac attaatttat aattgaggtg
      661 tccactttga aattagaatt taaaaaaagt atttccaata aaattattta cacgcttgtt
      721 gtgctattca cattcctttt cttacttggg tattttttac caataggtat agacaaagtt
      781 aaaagtttaa gctatagcca gtttttcttc agttcatata ctgttgcaac gcaattaggg
      841 ttcttactat tttcatttgt tattgcatat tttattaata aagagtattc gaataaaaat
      901 atcctgtttt ataaattgat tggcgataat atctttacct tcttctataa aaaggtagca
      961 gtgttatttt ttgaatgttt agtgttcata attctaagta tcactattat ttcaattata
     1021 tattcggatt tctcacatta cttactatta ataatcttat tttcactcgt tatcttacaa
     1081 tatatcttag ttgttggaac gattagtatg gtttcaccta acattttaat cagcttaggt
     1141 atcagcattg tatattggat tggttcagtc atcttagttg ccattaataa aaatatattt
     1201 ggtatcgttg ctccatttga ggcgagcaat acgatgtatc gtgccgtaga aaaaatattg
     1261 aataacgagt cagcatttat gtgtcctact gaaattataa atatagtatc attcttcgtt
     1321 cttctattta tagttaatac tattgtctta ttactaagta gaaagagatg gttgaaaatc
     1381 ggtatgtagt ataaaaaagg agcggtatac attgccgctt ctttttatta tgtattttta
     1441 caatctccac caatgacacg tcgtcattgc tacctcatac caaacaaaaa acagtctcgc
     1501 aatcaagact gttttccact caatatattc atccattagc gtaatagatt atttgacttc
     1561 tgtagctaca aagattttac gtttttccca aacgcctgtc ttttcattgt aatcatcaca
     1621 agtaattaat gttaattgtt tatctttacc tttttgttca tctagaactc ctacatctgt
     1681 aggcttaaca tctcttatac ttgtcatttt atacttacgt gtttcattac caactttaaa
     1741 gtacaccata ctaccttttt tggctgcttt aagatttgta aattgatagt tcggacggtc
     1801 aatgaaagtg tgtcctgcaa ttgaaatatt ttgatcatct agtgattcat tttcttctgc
     1861 aaagcttaca cctctattta attgttcagg tgttgctggt cctggatata ctggttcttt
     1921 aatatcagca tctggaattt caatatagcc tgccactttc gatttatctt tcggaatttg
     1981 aggtttagct tgctgctttt tatctttact cgcctgttct tttacatttt tatcatattg
     2041 ttcaatcttt tcatctttat ctttatcgtg aagataatta tcgatatgtg gtttagcaaa
     2101 caaatatgct gccactagga taagtaccac accagcgatt gtcattaatc gatttgtcca
     2161 ttttttcata cgttaaggct ccttttatac atttcacaat gcctgttaac atttcatata
     2221 gtataccatt aatttcaaaa tgactcatag caattcattt tatactataa aatttacatg
     2281 tatactttta cgttagattt cattacacat atttgcattc aaataacgaa acgcttttaa
     2341 taattactaa gggggaattg atatgattag atacgctaaa aaagaggatt taaacgctat
     2401 attagcgata tacaatgatg ccattatcaa tactacagct gtttatactt atgaaccaca
     2461 aaccatagac gaacgtgtcg catggtttga aacgaaacaa cgtaagcatg agcctatctt
     2521 tgtatttgag gaaaatggaa gtgtcttagg gtttgcgacg ttcggttcat ttagaccttg
     2581 gccagcatac ctatatacaa tcgaacattc tatttatgtc gatgcttcag ctagaggaaa
     2641 aggtattgct agtcaattac tacaccattt aattgtggaa gcaaaagcta aaggttatcg
     2701 tgcgctagtt gcaggcattg atgcttccaa caaagcgagt attcagttgc atcaaaagtt
     2761 tgcttttaag catgccggca cactgaccaa tgtaggtttt aaatttaata gatggttaga
     2821 tttagcattt tacgaattag atttacaaga ctagtaatgt ttgaatcaca taatataaac
     2881 aagacaacca tgttaattcc cttaacataa caagccaaca tataaaattt taaacttctc
     2941 aggggagtgg gacagaaatg ataaagagcc actaatgatt tattatgtag tggttcttac
     3001 acattagcca cagctaatgt gtacttaaaa ataggaatac atgagtaaaa ctcatgcata
     3061 agaaatacta atttctatag aaaaagtatt tctttatcgt cgtcccaccc caactcgcac
     3121 attattgtaa gctgactttt cgtcagcttc tgtgttgggg cccaaaaagc ttgttacaag
     3181 cgcattttcg ttcagtcaac tactgccaat ataactttgt agagcatatt acattgattt
     3241 acattgtccc ttttatttat tcttttcaaa tactatcccc atagctttga tttaacgctt
     3301 tttctcaata acaaaacgaa tatagtagaa catgaaaacg ataatcatgc tgagcgataa
     3361 agatttaaat aatagattga cccacgttcc ctcagtcgta tatccatatg taatcgttgt
     3421 gttaatgatg aatgctataa agatgattga tagtcttagc atatcatcac tccttttaag
     3481 ttattttaga tatacggggg cgcttttgca atcactattt tgattagtat gcattttcca
     3541 taaatctttc aacttcttca gagataatta agaagcatct atctggtact aatgatccag
     3601 acagatgctt cttttttatc aatattttat tgttatctca ttaattattt ttaaccatat
     3661 cttcagctgt gccaaagatt ttacgtttaa ttgcttcgcc agttggtgtg cctgctagtc
     3721 cacccaatcc agtttcacgt aatgatgcag gaaggttacg accaacctta tccattgctt
     3781 caataacttc atcaacaggg attctacttt caatacctgc taatgctaaa tctgctgaaa
     3841 ttaaagcgtt acccgaacca attgcatttc tcataacaca aggaatttca acaagtccgg
     3901 ctactggatc acaaactaaa cctaataaat tacttatcgc taatgccata gcgtgcccgg
     3961 atgcttctgg tgatcctccg aatatagcta ctgctgcagc tgcggccatt gcagatgctg
     4021 aaccaacttc agcttggcag ccacctgttg caccagctac acttgcattg tttgctacga
     4081 cacgcccaaa caatgctgaa gtgaataaga aatcaatcat ttgctcttct gttaaatcat
     4141 gtgttttttc taatttaaaa agtgcaccgg gaatggtacc cgaggaacca gctgttggcg
     4201 ttgcacaaat aatacccatc gcagcattga cttcatttgt tgcaatggca cctttgactg
     4261 cgtcaatcat ttcatatcca gacaaagcat gatgtgtttc attataatca cgtagtttag
     4321 cagcatcatg accagtgtag cccgttacac tttcaacccc atcacctgtc gtccctttga
     4381 ttactgcgtc tcgcatgaca tctaaatttt gtttcatttg cgctcgcact tcatcacgtg
     4441 atttaccgct taattccatt tcttctttaa ccatgatatc cgcaaatgac atattatttt
     4501 ctacggcata atctatagtc tctctaattg aatcaaacat gtttattccc cctctaattt
     4561 atataggaaa cgtttacgtc actgtatttc tctttaattg tatttaatgt tgattctgag
     4621 attgctttat ttaatggtat tacaaccaag catttatctt catctatctt aataaattca
     4681 tctttacagt ctaatttcat atcgttgata tcattgatga aatgatttac ttgtgcttta
     4741 gtcatatttc cgtcaacaac taaaattggt aatccatgat ttaaatctac ttctagtcca
     4801 tttatatgaa tacctttaat tttaattgta ccaccaccga ttgaaatacc gataatttca
     4861 atgtagcgac catcattacg agatgatttg atataagcac agtttggatg ttgaccaata
     4921 ctatcgcctt cttcttcgat gatatctatt ttaataccat catcagctgc aatttctaat
     4981 gaagatttaa ttcggttatc aaatgttgaa tatcccattg ctccacccac aatagcgaca
     5041 tctgtaccat gtccttggtg tgtttgagca aatgattcat aataatgtat ttcaatattt
     5101 ttaggaatat ctcccaatat tgcgcgtgct gaattcccaa tctttactgc accagccgta
     5161 tgagaacttg aagggcccat cataactggt ccgataatat cgaaagcact ttgataatca
     5221 tagctctttg ccataattaa acactctcct taatatgatt ctttttgctc ggcattttaa
     5281 agttgatatt cattaaatta aacttattaa ttagtgttcc aataatatag gctaagacaa
     5341 tgctgacaat aatcactgtt gcgattgtta caattgatgt cactgcatta ttaaagccaa
     5401 acaatacgat ggcgcctgca attggtgttg ccataccttt gacacctatt actagtccgc
     5461 taaatgtcac gatacatgcg ttgacgaccc caatcagtgc atttgtacca tatagttgta
     5521 ctggatattg cgctattaaa tcaatttgcg tcaatggctc aatacaaact gcaaatgctt
     5581 ttgacggtcc accaatgttt aattttcgga ataaaataag gttaacaaat gagctacctg
     5641 tacatgttag tgctccaata gccataggaa cacctgtcag tcctaataaa cttgttaata
     5701 ccattgaact tagcggtgtc atacctgtaa caggaatcac tagtcctaaa atgaccgcta
     5761 atgcatatgg attgttatca cctaccgcag tgacagcact acctatttgt tttaatgttg
     5821 ctagcacacc aggtgtaatg attgatgcaa gtccgaaagc aattgctggt gcaaataaga
     5881 tcaccacaat taagtccaag ccttctggaa ctttcttttc aatccattta attaaaaaag
     5941 ctacgccata agctgcgatg aatgctggta ataatttaaa gtcatgtaat actaaaccaa
     6001 caatgaccgc aaatactggt gcaacgccta agtttaagca cgttagaata cctactgcga
     6061 taccgcttaa acttcctgct aaatccccaa tatcttgtag aaatttaata tcaaatacgc
     6121 caccaatagc ataacttaag aatgcttgtg gtagaaatgt cgcacaagct gcaccggata
     6181 atgcttgtag tccttgttta ccgtacggtg catactttaa aaatagcgtc atgatcacta
     6241 aaaccaagac taatgtgcct acacctaaca gaatatccat ttcccaaaaa cctctctcta
     6301 tgtttatttt attttcagac cataaacatc gtacaccctt aagaaaacgt tttcaacttt
     6361 tatctg
//
LOCUS       DAKTIO010000149         6361 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.151, whole genome shotgun sequence.
ACCESSION   DAKTIO010000149 DAKTIO010000000
VERSION     DAKTIO010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.151"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            2..1519
                     /locus_tag="O0293_002212"
     CDS             2..1519
                     /locus_tag="O0293_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HCY0281083.1"
                     /translation="MSQQDKKLTGVFGHPVSDRENSMTAGPRGPLLMQDIYFLEQMSQ
                     FDREVIPERRMHAKGSGAFGTFTVTKDITKYTNAKIFSEIGKQTEMFARFSTVAGERG
                     AADAERDIRGFALKFYTEEGNWDLVGNNTPVFFFRDPKLFVSLNRAVKRDPRTNMRDA
                     QNNWDFWTGLPEALHQVTILMSDRGIPKDLRHMHGFGSHTYSMYNDSGERVWVKFHFR
                     TQQGIENLTDEEAAEIIATDRDSSQRDLFEAIEKGDYPKWTMYIQVMTEEQAKNHKDN
                     PFDLTKVWYHDEYPLIEVGEFELNRNPDNYFMDVEQAAFAPTNIIPGLDFSPDKMLQG
                     RLFSYGDAQRYRLGVNHWQIPVNQPKGVGIENICPFSRDGQMRVVDNNQGGGTHYYPN
                     NHGKFDSQPEYKKPPFPTDGYGYEYNQRQDDDNYFEQPGKLFRLQSEDAKERIFTNTA
                     NAMEGVTDDVKRRHIRHCYKADPEYGKGVAKALGIDINSIDLETENDETYENFEK"
     gene            1610..1759
                     /gene="rpmG"
                     /locus_tag="O0293_002213"
     CDS             1610..1759
                     /gene="rpmG"
                     /locus_tag="O0293_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCY0281084.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERIEMKKYCPRLNKYTL
                     HRETK"
     gene            2213..2482
                     /gene="rpsN"
                     /locus_tag="O0293_002214"
     CDS             2213..2482
                     /gene="rpsN"
                     /locus_tag="O0293_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCY0281085.1"
                     /translation="MAKKSKIAKERKREELVNKYYELRKELKAKGDYEALRKLPRDSS
                     PTRLTRRCKVTGRPRGVLRKFEMSRIAFREHAHKGQIPGVKKSSW"
     gene            2639..3616
                     /gene="guaC"
                     /locus_tag="O0293_002215"
     CDS             2639..3616
                     /gene="guaC"
                     /locus_tag="O0293_002215"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCY0281086.1"
                     /translation="MKIFDYEDIQLIPNKCIVESRSECDTTIQFGPKKFKLPVVPANM
                     QTVMNEKLAKWFAENDYFYIMHRFDEEARIPFIKHMQNSGLFASISVGVKKAEFDFIE
                     KLAQEKLIPEYITIDIAHGHSDSVINMIKHIKTHIPDSFVIAGNVGTPEGVRELENAG
                     ADATKVGIGPGRVCITKIKTGFGTGGWQLAALNICSKAARKPLIADGGIRTHGDIAKS
                     IRFGASMVMIGSLFAAHEESPGETVELDGKQYKEYFGSASEFQKGEHKNVEGKKMFVE
                     HKGSLMDTLKEMQQDLQSSISYAGGKDLKSLRTVDYVIVRNSIFNGDRD"
     gene            3638..4654
                     /locus_tag="O0293_002216"
     CDS             3638..4654
                     /locus_tag="O0293_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HCY0281087.1"
                     /translation="MKKFIVTVVAFLSIIIIAPITEFKPFIHLQNEVRQYIDIHINKE
                     TISAENKLDTPKKQQFAFNNIQMNMSKSDVEKTLNKPKRVTFNEYGTKWYTYYDDDYN
                     NFIMISYMKDKVNALYTNQNIITSKSKIKYNTPKSVVRQRLGEPETEIVKGRVRYEQN
                     NKEYDVFHKNHIYTTVFYDKHRRNNVTAVLQVSDAMENRLKEQYGAPSKSLADSFELQ
                     NFDLVNAERKQHQLSTLKYSKQNSETARKHSKDMANNHYFDHTNLKGQSPFDRLKKDG
                     ITFNSAGENLAYGQVSSIYAHQGLMNSIGHRKNILNDTFKILGVGVDFNDEKQPFWTE
                     NYTG"
     gene            complement(5034..5657)
                     /gene="lexA"
                     /locus_tag="O0293_002217"
     CDS             complement(5034..5657)
                     /gene="lexA"
                     /locus_tag="O0293_002217"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HCY0281088.1"
                     /translation="MRELTKRQSEIYNYIKQVVQTKGYPPSVREIGEAVGLASSSTVH
                     GHLSRLEEKGYIRRDPTKPRAIEIVSDQTNDNINMEETIHVPVIGKVTAGVPITAVEN
                     IEEYFPLPEHLTSTHNSDIFILNVVGDSMIEAGILDGDKVIVRSQTIAENGDIIVAMT
                     EEDEATVKRFYKEKNRYRLQPENSTMEPIYLDNVAVIGKVIGLYREM"
     gene            5800..6033
                     /locus_tag="O0293_002218"
     CDS             5800..6033
                     /locus_tag="O0293_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281089.1"
                     /translation="MFYNKYKNVSTYIIIFLVSSAAFAIFLLSANISAHSEQVYEMTD
                     HQIKNNTINKAYEHKDPTNNSEQRDGKVFALIN"
     gene            6169..>6361
                     /locus_tag="O0293_002219"
     CDS             6169..>6361
                     /locus_tag="O0293_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HCY0281090.1"
                     /translation="MSNSDLNIERINELAKKKKEVGLTQEEAKEQTALRKAYLESFRK
                     GFKQQIENTKVIDPEGNDVT"
BASE COUNT         2362 a          871 c         1146 g         1982 t
ORIGIN      
        1 tatgtcacaa caagacaaaa agttaactgg tgtttttggg catccagtat cagaccgaga
       61 aaatagtatg acagcagggc ctaggggacc tcttttaatg caagatattt actttttaga
      121 gcaaatgtct caatttgata gagaagtaat accagaacgt cgaatgcatg ccaaaggttc
      181 tggtgcattt gggacattta ctgtaactaa agatataaca aaatatacga atgctaaaat
      241 attctctgaa ataggtaagc aaaccgaaat gtttgcccgt ttctctactg tagcaggaga
      301 acgtggtgct gctgatgcgg agcgtgacat tcgaggattt gcgttaaagt tctacactga
      361 agaagggaac tgggatttag tagggaataa cacaccagta ttcttcttta gagatccaaa
      421 gttatttgtt agtttaaatc gtgcggtgaa acgagatcct agaacaaata tgagagatgc
      481 acaaaataac tgggatttct ggacgggtct tccagaagca ttgcaccaag taacgatctt
      541 aatgtcagat agagggattc ctaaagattt acgtcatatg catgggttcg gttctcacac
      601 atactctatg tataatgatt ctggtgaacg tgtttgggtt aaattccatt ttagaacgca
      661 acaaggtatt gaaaacttaa ctgatgaaga agctgctgaa attatagcta cagatcgtga
      721 ttcatctcaa cgcgatttat tcgaagccat tgaaaaaggt gattatccaa aatggacaat
      781 gtatattcaa gtaatgactg aggaacaagc taaaaaccat aaagataatc catttgattt
      841 aacaaaagta tggtatcacg atgagtatcc tctaattgaa gttggagagt ttgaattaaa
      901 tagaaatcca gataattact ttatggatgt tgaacaagct gcgtttgcac caactaatat
      961 tattccagga ttagattttt ctccagacaa aatgctgcaa gggcgtttat tctcatatgg
     1021 cgatgcgcaa agatatcgat taggagttaa tcattggcag attcctgtaa accaacctaa
     1081 aggtgttggt attgaaaata tttgtccttt tagtagagat ggtcaaatgc gcgtagttga
     1141 caataaccaa ggtggaggaa cacattatta tccaaataac catggtaaat ttgattctca
     1201 acctgaatat aaaaagccac cattcccaac tgatggatac ggctatgaat ataatcaacg
     1261 tcaagatgat gataattatt ttgaacaacc aggtaaattg tttagattac aatcagagga
     1321 cgctaaagaa agaattttta caaatacagc aaatgcaatg gaaggcgtaa cggatgatgt
     1381 taaacgacgt catattcgtc attgttacaa agctgaccca gaatatggta aaggtgttgc
     1441 aaaagcatta ggtattgata taaattctat tgatcttgaa actgaaaatg atgaaacata
     1501 cgaaaacttt gaaaaataaa tttgatatgt agtttctata ttgcgtagtt gagcagttta
     1561 tgatatcata ataaatcgta aagattccta acaagagagg gtgtttaacg tgcgcgtaaa
     1621 cgtaacatta gcatgcacag aatgtggcga tcgtaactat atcactacta aaaataaacg
     1681 taataatcct gagcgtattg aaatgaaaaa atattgccca agattaaaca aatatacgtt
     1741 acatcgtgaa actaagtaat tcttatcatt caaatacgac gatttgaaaa taaagcgggc
     1801 ttacctatta tattggggag ctcgcttttt tatgaaattt ttgtgaagag tgattaatgg
     1861 attgagtttc atcggtagaa caatatatga ttatattagt tgttacttta ttaaaatttg
     1921 agaatattta tagaaggaaa tagattactg attttataaa gtcactttgt tagcgaatgc
     1981 ttgaaagagt atttaatata gtagaattta aaatttcaaa gcggaattta ataagtacga
     2041 agtaattctg ggtatgtttt ataaatgttc gataatacac tttaatctta aatatgatgg
     2101 tttagaaaat gatttaacaa agaaatgaaa ctttactgtt gaattatgtg aggattgtgt
     2161 tattatataa atcgtaataa ttacgatttg ataaaaagtg aggtaactat atatggctaa
     2221 gaaatctaaa atagcaaaag agagaaaaag agaagagtta gtaaataaat attacgaatt
     2281 acgtaaagag ttaaaagcaa aaggtgatta cgaagcgtta agaaaattac caagagattc
     2341 atcacctaca cgtttaacta gaagatgtaa agtaactgga agacctagag gtgtattacg
     2401 taaatttgaa atgtctcgta ttgcgtttag agaacatgcg cacaaaggac aaattccagg
     2461 tgttaaaaaa tcaagttggt aaaatcaaat ttcgtacttt agcccattta caatatcaat
     2521 aaaacaatgt acagtatata cgaatgctat aaactgaatg ttttctcata ttaataagga
     2581 aacattcggt ttttaatttg catttaaaaa taattagttt ttaaaggggc tatttaaagt
     2641 gaaaatattt gattacgaag atattcaatt aatacctaat aaatgcatag ttgaaagtag
     2701 gtctgaatgt gatacaacta tccaatttgg tccgaaaaaa ttcaagctac ctgtagttcc
     2761 tgcaaatatg caaacagtta tgaatgagaa attagcgaaa tggtttgctg aaaatgatta
     2821 cttttatatc atgcatcgtt ttgatgaaga agcaagaata ccttttataa aacatatgca
     2881 aaattcaggc ttatttgcat ctatttcagt tggtgtaaag aaagcggaat ttgattttat
     2941 tgaaaagtta gctcaagaaa aattaatccc cgaatatatt acaatagata ttgcgcatgg
     3001 tcactcagat tcagtgataa acatgattaa acatataaaa acccatatac ctgatagttt
     3061 tgttattgct ggtaatgttg gtacgccaga aggtgttaga gaattagaaa atgctggtgc
     3121 tgatgctacc aaagtcggta taggtcctgg tagagtttgt attacaaaga ttaaaacagg
     3181 ttttggtact ggtggttggc agttagcggc attaaacata tgtagtaaag cagctcgtaa
     3241 acctttgatt gccgatggtg gtataagaac gcatggcgac attgctaaat caattagatt
     3301 tggtgcatca atggtcatga ttggttcatt atttgcggca cacgaagaat cacctggtga
     3361 aactgtagaa cttgatggta aacagtataa agaatatttt ggtagtgcat ctgaatttca
     3421 aaaaggcgaa cataaaaatg tagaaggtaa aaaaatgttt gtagaacata agggttcatt
     3481 aatggatacc ttaaaagaaa tgcaacaaga tttacaaagc tcaatttcat atgccggtgg
     3541 aaaagacttg aaatcattac gtactgtaga ttatgttatt gttagaaact ctattttcaa
     3601 cggtgataga gattaatatt tatagtaggt gatgtaaatt aaaaaattca tagtaactgt
     3661 tgttgcgttt ttatcaatta ttatcattgc gccaataaca gaatttaaac cattcattca
     3721 tttacaaaat gaagtaagac aatatattga cattcacatc aataaagaaa caatttctgc
     3781 ggaaaataaa ttggatacac cgaagaaaca acaatttgcc tttaataata tacaaatgaa
     3841 catgtcgaaa tcagatgttg agaaaacatt aaataaacca aaaagagtga catttaatga
     3901 atatggtacg aagtggtata cgtattatga tgacgattac aataatttta taatgataag
     3961 ttacatgaaa gataaagtta atgcgttata tacaaatcaa aatataatca cttcaaaatc
     4021 aaaaattaaa tataatacac ctaaatcggt tgtaaggcaa agattaggcg aaccagaaac
     4081 agagattgtt aaaggtagag tgcgttacga acaaaataat aaagaatatg atgttttcca
     4141 taaaaatcac atttatacga cggtatttta tgataagcat cgacgtaata atgtaacagc
     4201 tgttttacaa gtaagtgatg ctatggaaaa tagattaaaa gaacaatatg gagcaccatc
     4261 gaaatcgctt gcagatagtt ttgaactaca aaattttgat ttagttaatg ctgaaagaaa
     4321 acaacatcaa ttatctacat tgaagtattc taaacagaat tctgaaactg cacgtaagca
     4381 tagtaaagat atggccaaca atcattattt tgatcataca aatttaaaag gtcaatcacc
     4441 atttgatcga ttgaaaaaag atggtattac atttaactca gccggagaga atttagcata
     4501 tggtcaagtt agtagtatct atgcacatca aggattaatg aattctattg gtcacagaaa
     4561 aaatatttta aatgatacgt ttaaaatatt aggtgttggt gttgatttta atgatgaaaa
     4621 acaacctttt tggacagaaa attatactgg ttaatgtttt tggcatgaga ttaaagggta
     4681 atgtttgtct gtaaggaagt agataaacat gatgacaaca gataaaccaa aagatgcgga
     4741 tatacttgaa cgcgtcaaag atatacttaa taaaaaagaa agaaaaaaat aataatggct
     4801 cgctcctgta aattattacg ggggcgagtt ttaatttgtg taacattttg cagaaatctg
     4861 taattcaaaa tttatattaa agaatgagat tatctaacaa ttcgcaattt ctgtttgaac
     4921 tgaatttaga ttagaataaa taaaacctct tgaaaacgta actttttcaa gaggtccaaa
     4981 atttataaaa atgagggctt aacaattacg ataatatata acggtttaaa atattacatt
     5041 tcgcggtaca aaccaattac tttcccaatt acagcaacat tgtctaggta aattggctcc
     5101 attgtactat tttcaggttg taatcgataa cgattttttt ctttatagaa gcgtttgaca
     5161 gttgcttcat cttcctcagt catagcaaca ataatgtctc cattttctgc tatggtttga
     5221 ctgcgaacaa ttactttgtc tccgtctaat ataccagcct caatcatact gtcgcctacg
     5281 acgtttaata tgaatatgtc gctattgtgt gtcgatgtta agtgttcagg taatggaaaa
     5341 tattcttcaa tattttctac tgcggtaata ggaacacctg ctgtgacttt accaataact
     5401 ggcacatgaa tcgtttcttc catattaata ttatcatttg tttgatcact tacaatttct
     5461 atagcacgtg gtttcgttgg atctcttctt atatagcctt tttcttcaag acgtgaaagg
     5521 tgaccatgaa cagttgaact ggatgctaag ccaactgctt caccaatttc gcgaacacta
     5581 ggcggataac ctttcgtttg aacaacttgt ttaatatagt tatatatttc gctttgtcgt
     5641 tttgttaatt ctctcatata taggcactcc ctaaattaat ttaaatacag tatatcatta
     5701 atttgaaacc aaaacaaaca tttgttcgtt aaaatattga cacagaacat aagttctgat
     5761 aaattactaa tacaaacaaa tgttctagga gtgaaaatga tgttttacaa taaatataaa
     5821 aacgtatcaa catatatcat catattttta gtttcaagtg cagcctttgc aatattcttg
     5881 ttaagtgcga acattagtgc tcactcggaa caagtgtacg aaatgactga ccatcaaatt
     5941 aagaacaata cgataaataa agcatacgaa cataaagacc ctacaaacaa tagcgaacaa
     6001 agagatggga aagtgttcgc tttaataaat tgatacattg tcacaacgtt attttgccta
     6061 tttttgcgaa atagcgtttt ttattacttt tttgctgatc ttaaatttgt tatattttgt
     6121 taaagtatta taatgattga ataaacaaat tgaaggtagg ttttttaatt gagtaattct
     6181 gatttgaata tcgaaagaat taacgagtta gctaaaaaga aaaaagaagt aggattaact
     6241 caagaagaag caaaggagca aacagcctta agaaaagctt atcttgagag ttttagaaaa
     6301 gggtttaaac aacaaattga aaatactaaa gtaattgatc cagaaggtaa tgatgtaaca
     6361 c
//
LOCUS       DAKTIO010000150         6341 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.152, whole genome shotgun sequence.
ACCESSION   DAKTIO010000150 DAKTIO010000000
VERSION     DAKTIO010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6341
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.152"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..534
                     /locus_tag="O0293_002220"
     CDS             <1..534
                     /locus_tag="O0293_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0281091.1"
                     /translation="FIIIIQFACILINTGLWRVVLPLFISNILKEGVGVYGLATSCLG
                     IASLLMSLIMGLLSEKRLIFKFSIGVLVWGIGLSIINVFPSVAILYIGATLLGLGQSI
                     EGLTRSVAIQIKTPNHLMGKVFSISSTSNYAADTLSLGLISIIIPLLSLSNIFILGGV
                     IISILSLAGLKFVKKSM"
     gene            515..748
                     /locus_tag="O0293_002221"
     CDS             515..748
                     /locus_tag="O0293_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281092.1"
                     /translation="MLRSLCKHYRILINAIKVGIEMKYKISLAYNLAIIIGSLIILCI
                     LISRGYDIYVILIPILTILASLINLFCDIKKHK"
     gene            complement(856..1164)
                     /locus_tag="O0293_002222"
     CDS             complement(856..1164)
                     /locus_tag="O0293_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281093.1"
                     /translation="MTVNIVFSIVFCISMVILGIYVAITKDFTLISFINQTAIADKHK
                     NQIAYIFTLCISLSAVFLMSSILSFEYDFIALAFLFLTIALLLIALFYVCFYKITKYP
                     "
     gene            complement(1308..1574)
                     /locus_tag="O0293_002223"
     CDS             complement(1308..1574)
                     /locus_tag="O0293_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281094.1"
                     /translation="MKGSKQILLIMGIISLIVLFIFTLFIMAQYAKHYEQKSDSSNAH
                     TLNSSSAIIEQHTMSNLASLDLYAPVRNITSSRDIHPIYFMTKD"
     gene            complement(1858..3666)
                     /locus_tag="O0293_002224"
     CDS             complement(1858..3666)
                     /locus_tag="O0293_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCY0281095.1"
                     /translation="MHFHTHLLYNTCEKYCLGAVFFYFWGVFLYNSLHKCKGFSFHVL
                     LSLTEILKGCLATMDKELWIERANDSLVKHFYEQQSDIEQREGFESKLTFGTAGIRGK
                     FGLGEGRLNKFTIEKLALGLARYLNAQTNSPTIVIHYDIRHLSTEFAQIIANVLANHQ
                     ITVYLPDTYKTTPELSFAVRNLNTTAGIMITASHNPKDYNGIKVYGSDGAQLSTDASE
                     LASRYIEEVGDPLQIDIPISKQNTSYIKPFPKSVTDDYMKHIQNMIGYIPKSDLQVVF
                     TSLHGTSVPIVPELLQSLNFNQFNLVEAQCKPDPNFSSVQSANPEDHRAFDQAVELAN
                     KSHADLLISTDPDADRLGIAERDAHGHITYFNGNQIGALLLNYRIQQTSQLRHRLMIQ
                     SIVSSELTKSLARYNNVEYKEVLTGFKFIAQEIRQLDDHQNMIFAFEESYGFLSEPFV
                     RDKDAVQIVPLIIKYASELKLYGKTLKDELEQIYQTVGRHEDTLFSHTLEGFEGKKKI
                     NAIMTKFRSNPPQEIQGLKVKAIEDYLTSEVYHLDKDTTSQINSPKSNVIRVLFDEGF
                     IALRPSGTEPKIKLYVSLKCPNFDDVAQKINAMIFS"
     gene            3783..4175
                     /locus_tag="O0293_002225"
     CDS             3783..4175
                     /locus_tag="O0293_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HCY0281096.1"
                     /translation="MKKVINVVGAIIFSDNKILCAQRSEKMSLPLMWEFPGGKVEKNE
                     TEKDALIREIREEMKCDLIVGDKVITTEHEYDFGIVRLTTYKCTLNKELPTLTEHKSI
                     EWLSINELDKLNWAPADIPAVNKIMTEG"
     gene            4177..>6341
                     /locus_tag="O0293_002226"
     CDS             4177..>6341
                     /locus_tag="O0293_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCY0281097.1"
                     /translation="MSRLLNDFNQSLHKGFIDKHISHKGNYTPKLLVNNKNEKVLSTI
                     IDELQKCETFYFSVAFITESGLASLKAQLLDLSNKGVKGKILTSNYLGFNSPKMYGEL
                     LKLKNVEVRLTDIAGFHAKGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKN
                     GDLVDSVKSEFDLLWQKSTPLTEQWINSYKESFEYRSLEKLAEVEQTQMLLADKVKKS
                     VEIVPNLMQAEALRSLKAIRDKAKDKALIISATGTGKTILCALDVREVNPNKFLFIVH
                     NEGILNRAKEEFKKVLPIKNDSDFGLLTGKHRDVDAKYLFATIQTLSRDDNFKQFDEN
                     EFDYIVFDEAHRSAASTYQRVFNYFKPKFMLGMTATPERSDELSIFELFDYNIAYEIR
                     LQAALESDILCPFHYFGVTDYVHQGIKEDDVTKLRYLTSDERVNYIIQKTDYYGYSGE
                     ILQGLIFVSSKKEAYDLADKLSSKGIKSVALTGDDSVNYRQIVIEKLKEGKINYIITV
                     DLFNEGIDIPEVNQVVMLRPTESSIIFIQQLGRGLRKSSNKEYVTVIDFIGNYKTNYL
                     IPIALSGDQSQNKDNYKKFLTNNDSINGVSTINFEEVAKKQIYNSLDAVSLNQNKLIL
                     KAYEEVENRLGHMPLLMDFIQQHSIDPSVIFSKFSNYYEFLVRYKKIDTLLTENESKN
                     LVFFSRQIAPGLKRIDSLVLEELLKNELTYDELKNKMLN"
BASE COUNT         2276 a          829 c         1077 g         2159 t
ORIGIN      
        1 tttattataa tcatccaatt tgcctgcata ttgattaata ctggcctttg gcgtgttgta
       61 ttaccattgt ttatttccaa tatattaaaa gagggcgttg gtgtatatgg actagctaca
      121 tcatgtttag gtatagcatc tttattaatg tcattgatta tgggtttgct gtcagaaaag
      181 cgattaatct ttaaatttag tattggtgtt ttagtttggg gaattggctt atcgattatt
      241 aatgtgtttc caagtgtggc gatattatat ataggtgcaa cattattggg acttggacaa
      301 tctatagagg gactaacaag atcagttgcc attcaaataa aaacgccaaa tcatttgatg
      361 gggaaagtat ttagtatctc atctacatcg aattatgcag cggatacatt gtctttagga
      421 ctgataagta taattatacc tttactaagc ttaagtaata tatttatatt aggaggtgta
      481 attatttcaa tacttagctt agctgggtta aagtttgtta agaagtctat gtaaacatta
      541 tagaatttta ataaatgcta ttaaggtggg aattgaaatg aaatataaaa ttagtttggc
      601 ttataattta gcaattataa tagggtcgct aattatatta tgtatactaa tatctagagg
      661 ttatgatatt tatgtaatct tgattccaat tttgactatt ttagctagtt taatcaattt
      721 gttttgtgat attaaaaagc ataaatgatt ctatattaat gcaactttgt attcgtacaa
      781 ggttgtattt ttttcgcaaa caattactta ctgacagcgc atcaataaat ctaaccaaca
      841 cactatcaca aacatttatg ggtattttgt aattttataa aagcaaacgt agaataatgc
      901 gataagtaat aatgctatcg ttaaaaataa gaatgctaat gcaataaaat catattcaaa
      961 gcttagaatt gaagacatta aaaatactgc actcaaactt atacatagcg taaatatata
     1021 tgcaatttgg ttcttgtgtt tatctgcaat ggccgtttga tttataaaag agattagtgt
     1081 gaaatcttta gttattgcga cataaatgcc taatataacc atacttatgc agaatacgat
     1141 actaaatacg atgttgacgg tcattgttgc accttcctga tagaatataa tgtactgtct
     1201 tttagtaatg gatgcgtttc ttatatatgt atgtttctaa aaaacagtac gtttttatca
     1261 ttttttaatt tattagttga atgaatgggg ttatgaaaaa ggaagtttta atcttttgtc
     1321 atgaaataaa tgggatgaat atcacgacta gaagtaatgt tacgaacagg agcgtataaa
     1381 tctagagacg ctaaattcga catagtatgt tgctcaatta tggctgatga tgaatttaaa
     1441 gtatgtgcgt tggaactgtc ggatttttgt tcataatgtt ttgcatattg cgccatgatg
     1501 aatagtgtaa aaataaataa aacaataaga gatataatgc ccataatcaa aagtatttgt
     1561 ttagagcctt tcattatttc acatcctttc taaaatatat ttgtaactaa atttaaaata
     1621 gttatttttg taattctaaa cctttttcat cgcgaaaaca attaaatagg tcgcggtatt
     1681 aattattata ttattaccgc ttaatatgaa aaatacatga aaattaattt tctaatatac
     1741 ttttgaaaaa ttattacaaa ttagcccctt caaaacgcga aaacataagg attctagttt
     1801 caaaagggct gataagcata aaatgaaatg taatatttcg atgtataaaa tttttaatta
     1861 gctaaaaatc atcgcattaa ttttttgagc tacatcatca aaattcggac attttaacga
     1921 cacatataat ttaattttag gttcagtacc agaaggacgt aaagcgataa atccttcgtc
     1981 aaataagaca cgaataacat ttgatttagg agaattaatc tgcgacgttg tatctttatc
     2041 caaatgataa acctcgctag ttaaataatc ttcaattgct ttcactttga gtccttgaat
     2101 ctcttgcggt ggatttgaac ggaatttggt cattattgca ttaattttct ttttcccttc
     2161 aaatccttct agcgtatgcg agaataatgt atcctcatgt ctaccaacag tttgataaat
     2221 ctgttctaat tcatctttca atgttttgcc atataatttt aactcagaag cgtattttat
     2281 aatgagtggc acaatttgta cggcatcttt atcacgtaca aaaggctctg atagaaaacc
     2341 gtaactctct tcaaatgcga aaatcatatt ttgatgatca tccagttgtc ttatttcctg
     2401 agcaataaat ttaaagcccg tcagcacctc tttgtattca acattattat agcgtgctaa
     2461 tgactttgtt aactcactac ttacaataga ttgaatcatt aagcggtgac gtaattgaga
     2521 cgtttgttga atacgataat taagtaacag cgcaccaatt tggttgccat taaaatatgt
     2581 gatatgaccg tgtgcatcac gttcagcaat accaagtcga tctgcatcag ggtcagttga
     2641 tattaataag tctgcatgac ttttatttgc aagttcgact gcttgatcaa aagcgcgatg
     2701 atcttcagga ttagcacttt gaactgagct gaaattcgga tcaggtttac attgtgcttc
     2761 tacaagatta aattgattga aattcaaaga ttgtaataac tcaggtacaa tcggaacact
     2821 tgtaccatgc aaactagtga acacaacttg taaatcagat ttaggaatat agccaatcat
     2881 attttgaatg tgtttcatat agtcatcagt tacagatttc ggaaaaggct tgatataaga
     2941 tgtattttgt ttagaaatag gtatatcaat ctgtaatgga tcgccaactt cttcgatata
     3001 acgacttgca agctcagatg catcagtcga taattgcgca ccatcagaac catatacttt
     3061 gatgccgtta tagtctttcg gattatgact tgctgtaatc ataatgccag cagtagtatt
     3121 aagattgcgc accgcgaacg ataattccgg tgtcgtttta tatgtatcag gtaaataaac
     3181 tgttatttga tggtttgcta atacattagc aattatttgg gcgaattcag ttgaaagatg
     3241 tctaatatca taatgaatga ctattgttgg actgtttgtt tgggcattta aataacgcgc
     3301 taaacctaat gccaattttt caatagtaaa cttattaagt cgaccttcac caagaccgaa
     3361 ttttccgcgt atacccgcag taccaaatgt taatttactt tcaaaacctt ctcgctgttc
     3421 aatgtcagat tgctgctcat aaaaatgttt aactaaacta tcattagctc gttctatcca
     3481 taattcttta tccattgttg ctaaacatcc tttcaaaatc tcagttagac ttaataaaac
     3541 atgaaaacta aagcccttac atttatgtaa tgaattataa agaaatacgc cccaaaagta
     3601 aaaaaacaca gccccaagac aatacttttc acaagtatta tataatagat gtgtatgaaa
     3661 atgcatggag tagagcgttc atgtgaatgt aaaatattaa tttttaatat tgatttatat
     3721 atagttttgt aatttttgat agaataacaa ttaagatgat tttaaattga agggtgagtc
     3781 ttatgaaaaa agtaatcaat gtagtaggag ctattatttt ttctgataac aaaattcttt
     3841 gtgcacagag aagtgaaaaa atgagtctgc ctttaatgtg ggaatttcct ggcggtaagg
     3901 ttgaaaagaa tgaaactgaa aaagacgctt tgattagaga aattagagaa gaaatgaaat
     3961 gtgatttaat tgttggagac aaagttataa ctacagaaca tgaatatgat tttggaattg
     4021 ttaggttaac aacatacaaa tgtactttaa acaaagagtt accaactcta actgaacata
     4081 agagtattga atggttgtca ataaacgaac ttgataaatt aaattgggcc ccagcggata
     4141 taccagccgt taataaaatt atgactgagg gataatatga gtagattact aaatgatttc
     4201 aatcaatctt tacataaagg gtttatagac aaacatattt ctcataaagg gaattataca
     4261 ccgaagcttt tagtaaataa taaaaatgaa aaagttcttt caacaattat agatgaactt
     4321 caaaaatgtg aaacgtttta tttttctgtg gcttttataa cagaaagcgg tttagccagc
     4381 ttaaaggctc aactattaga tttaagcaat aagggtgtta aagggaaaat attaacatct
     4441 aattacttag ggtttaatag tcctaaaatg tatggagaat tacttaaatt aaaaaatgta
     4501 gaggtaagat taactgatat tgctggattc catgccaaag gatatatttt tgagcataag
     4561 gattatagtt ctatggtaat aggaagctct aatttaacat ctaatgcgtt aaaggttaat
     4621 tatgagcata atgttttact gtctactatg aaaaatggcg atttagttga tagtgtaaaa
     4681 agtgaatttg acttgttatg gcaaaagagt accccactga ctgagcaatg gattaattca
     4741 tataaagaat catttgagta ccgttcctta gaaaaattag cggaggttga acaaactcaa
     4801 atgctgctag ctgataaggt gaaaaaatca gtggaaattg tacccaattt aatgcaagca
     4861 gaagcattaa ggtcattaaa agctattagg gataaagcca aagataaggc gttaataata
     4921 tctgcaactg gtacaggtaa aacgatttta tgtgcattag atgttagaga agtaaaccct
     4981 aataaatttt tatttattgt tcataatgag gggattttaa atagagctaa ggaagaattt
     5041 aaaaaagtat tacctataaa aaatgatagt gattttggat tgttgacagg aaaacataga
     5101 gatgtcgatg ccaaatattt atttgcaacc attcaaacat tatctagaga tgataatttt
     5161 aagcaatttg atgaaaatga atttgattat attgtttttg atgaagcaca tcgttctgct
     5221 gcatctactt atcaacgagt atttaactac ttcaaaccta agttcatgct gggaatgact
     5281 gctacaccag aaagatcaga tgaattaagt atatttgaac tgtttgatta taatattgca
     5341 tatgaaataa ggttacaagc agcattagaa agtgatattt tatgtccatt tcattatttt
     5401 ggtgtgactg attatgtaca tcaaggtatt aaagaagatg atgtaactaa actgagatat
     5461 ttaacttctg atgaaagagt taattatatt attcaaaaga cagattacta tggatattca
     5521 ggtgaaattt tacaaggatt gatttttgta agtagcaaaa aagaagctta tgatttagcg
     5581 gataaactaa gttcaaaagg gattaaaagt gttgctttaa caggagatga tagtgttaat
     5641 tatcgccaaa ttgtaataga aaaattaaaa gaaggtaaga ttaactatat tataactgta
     5701 gatcttttta acgaaggtat tgatattcca gaagtcaatc aagttgtaat gttgagacca
     5761 acagagtcta gtattatatt cattcagcaa cttggtagag gattaagaaa aagttcaaat
     5821 aaagagtatg ttacagtaat tgattttatt ggtaattata agacaaatta tttaattccg
     5881 attgcgcttt ctggggatca atcgcaaaat aaagataatt ataagaagtt tttaacgaat
     5941 aacgattcga ttaatggagt atctacaatt aattttgaag aagttgctaa aaaacagatt
     6001 tacaattcat tggatgcagt atcattaaat caaaataaat taatattaaa agcttatgaa
     6061 gaagttgaaa atagattggg acacatgccg ttactaatgg atttcataca acaacattct
     6121 atagatccaa gcgttatatt ttctaaattt agtaattatt acgagttctt agtgagatat
     6181 aaaaaaatag atacattatt gacggaaaat gaatctaaaa atctggtttt cttttcaagg
     6241 caaatagcac ctggattgaa aagaatcgat agtttagttt tagaagaact tttaaaaaat
     6301 gaattaacat atgatgaatt aaaaaataaa atgttgaacg a
//
LOCUS       DAKTIO010000151         6279 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.153, whole genome shotgun sequence.
ACCESSION   DAKTIO010000151 DAKTIO010000000
VERSION     DAKTIO010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6279
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.153"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            93..2081
                     /gene="tkt"
                     /locus_tag="O0293_002227"
     CDS             93..2081
                     /gene="tkt"
                     /locus_tag="O0293_002227"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCY0281098.1"
                     /translation="MFNEKDQLAVDTLRALSIDTIEKANSGHPGLPMGAAPMAYTLWT
                     RHLNFNPQSKDYFNRDRFVLSAGHGSALLYSLLHVSGSLELEELKQFRQWGSKTPGHP
                     EYRHTDGVEVTTGPLGQGFAMSVGLALAEDHLAGKFNKEGYNVVDHYTYVLASDGDLM
                     EGISHEAASFAGHNKLSKLVVLYDSNDISLDGELNKAFSENTKARFEAYGWNYLLVKD
                     GNDLEEIDKAITTAKSQEGPTIIEVKTTIGFGSPNKAGTNGVHGAPLGEVERKLTFEN
                     YGLDPEKRFNVSEEVYEIFQNTMLKRANEDESQWNSLLEKYAETYPELAEEFKLAISG
                     KLPKNYKDELPRFELGHNGASRADSGTVIQAISKTVPSFFGGSADLAGSNKSNVNDAT
                     DYSSETPEGKNVWFGVREFAMGAAVNGMAAHGGLHPYGATFFVFSDYLKPALRLSSIM
                     GLNATFIFTHDSIAVGEDGPTHEPIEQLAGLRAIPNMNVIRPADGNETRVAWEVALES
                     ESTPTSLVLTRQNLPVLDVPEDVVEEGVRKGAYTVYGSEETPEFLLLASGSEVSLAVE
                     AAKDLEKQGKSVRVVSMPNWNAFEQQSEEYKESVIPSSVTKRVAIEMASPLGWHKYVG
                     TAGKVIAIDGFGASAPGDLVVEKYGFTKENILNQVMSL"
     gene            2359..2601
                     /locus_tag="O0293_002228"
     CDS             2359..2601
                     /locus_tag="O0293_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HCY0281099.1"
                     /translation="MATWLAIIFIVAALILGLIGGFLLARKYMMDYLKKNPPINEEML
                     RMMMMQMGQKPSQKKINQMMTMMNKNMDQNMKSAKK"
     gene            2588..2686
                     /locus_tag="O0293_002229"
     CDS             2588..2686
                     /locus_tag="O0293_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281100.1"
                     /translation="MRKSKFAIDRGYFPDMEMASFYNQINKNKYVY"
     gene            2780..3247
                     /locus_tag="O0293_002230"
     CDS             2780..3247
                     /locus_tag="O0293_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdC family protein"
                     /protein_id="HCY0281101.1"
                     /translation="MLYLIFSIIVALFMGTIVIVIRMKAQNYPVNEKKIVLPPFFMAT
                     GALMYVVPYFRLTGSEMLEAFIIGLLFSTVLIWTSRFEVKGTEIYMKRSKAFPVILIS
                     LLIIRTVMKIFISNEIDPGELGGMFFLLAFCMIVPWRAAMLYKYKKLKKTLIN"
     gene            3371..4492
                     /locus_tag="O0293_002231"
     CDS             3371..4492
                     /locus_tag="O0293_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCY0281102.1"
                     /translation="MKIIHTADWHLGKILNGKQLLEDQAYILDMFVEKMKEEEPDIIV
                     IAGDLYDTTYPSKDAIMLLEQAIGKLNLELRIPIIIISGNHDGKERLNYGASWFEHNQ
                     LFIRTDFTSINSPIEINGVNFYTLPYATVSEMKHYFEDDTIETHQQGITRCIETIAPE
                     IDEDAVNILISHLTVQGGKTSDSERPLTIGTVESVQKGVFDIFDYVMLGHLHHPFSIE
                     DDKIKYSGSLLQYSFSEAGQAKGYRRVTINDGIINDVFIPLKPLRQLEIISGEYNDVI
                     NEKVHVKNKDNYLHFKLKNMSHITDPMMSLKQIYPNTLALTNETFNYNEENNAIEISE
                     KDDMSIIEMFYKHITDKELSDIQSKKIKNILENELRKED"
     gene            4496..>6279
                     /locus_tag="O0293_002232"
     CDS             4496..>6279
                     /locus_tag="O0293_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCY0281103.1"
                     /translation="MKPLHLKLNNFGPFLKEEIDFSKIDNNELFLISGKTGSGKTMIF
                     DAMTYALFGKASTEQREENDLRSHFADGKQPMSVTFEFQLNHRIYKVHRQGPYIKEGN
                     TTKTNAKFDVFEMVDGKYEIRESKVISGTQFIIELLGVNADQFRQLFILPQGEFKRFL
                     ISNSREKQGILRTLFDSEKFEAIREILKEEVKKEKAQIENRYQQIDLLWQEIESFDDD
                     NIKGLLEVATQQIDKLIENIPLLQARSKEILASVNESKETAIKEFEIIEKKTLENNIL
                     KDNINQLNKNKIDFVQLKEQQPEIEGIEAKLKLLQDITNLLNYIENREKIETKIANSK
                     KDISKTNNKILNLDCDKRNIDKEKKMLEENGDLIESKISFIDKTRVLFNDINKYQQSY
                     LNIERLRTEGEQLGDELNDLIKGLETVEDSIGNNQSDYEKIIELNNTITNINNEINII
                     KENEKAKAELDKLLGSKQELENQINEETSILKNLEIKLDRYDKTKLDLNDKESFISEI
                     KSAVNIGDQCPICGNEIQDLGHHIDFDSIAKRQNEIKEIEANIHAIKSNIAVHNSEIK
                     FVNEKISNINIKTQSDFSLEVLNKRLLE"
BASE COUNT         2402 a          797 c         1146 g         1934 t
ORIGIN      
        1 attataactt gaaataagta ttttacttat ctttttattt taaaataagt tataatgtat
       61 ttgataaaat tgaagaaggg aagatacaca agatgtttaa cgaaaaagat caattagctg
      121 ttgatacgct acgtgcacta agtatcgaca caatcgaaaa agcgaattct ggtcatccag
      181 gattacctat gggagctgcc ccaatggctt acactttgtg gacacgtcat ctgaatttta
      241 atccacaatc taaagattac ttcaatagag accgtttcgt attatctgca gggcatggtt
      301 cagcattatt gtatagcttg ttacatgttt ctggtagttt agaattagaa gaattaaagc
      361 aatttagaca atggggttct aaaacaccag gtcatcctga atacagacat acagatggtg
      421 tagaagttac taccggacca cttggacaag gttttgctat gtcagtagga ttagctttag
      481 cagaagatca cctagcaggg aaatttaata aagaaggata taatgttgta gatcattaca
      541 catatgtatt agcttctgac ggtgatttaa tggaaggtat atcgcatgaa gcagcttcat
      601 ttgctggaca taataaatta agtaaattag ttgttttata cgattcaaat gatatttcat
      661 tagatggcga attaaacaaa gctttttctg aaaacacaaa agctcgtttt gaagcatatg
      721 gttggaatta cttactagtt aaagatggta atgatttaga agaaattgat aaagcgatta
      781 ctacagctaa atctcaagaa ggaccaacga ttattgaagt taaaacaaca atcggatttg
      841 gttcaccgaa taaagcagga actaatggtg ttcatggggc acctttaggt gaagttgaaa
      901 gaaaattaac attcgaaaat tacggtttag atcctgaaaa acgttttaat gtttcagaag
      961 aggtatacga aattttccaa aatactatgt taaaacgtgc taatgaagat gaatctcaat
     1021 ggaattcatt attagaaaaa tatgcagaaa catatcctga attagcagaa gaatttaaat
     1081 tagcgattag tggtaaattg cctaaaaatt ataaggatga attaccacgt tttgaactgg
     1141 gtcataatgg tgcatctcgt gctgattctg gtactgttat tcaagcaatc agtaaaactg
     1201 tcccttcatt ctttggtgga tcagcagacc ttgctggttc aaacaaatcc aatgtaaatg
     1261 atgcaactga ttatagttct gaaacacctg aaggtaaaaa tgtgtggttt ggtgtacgtg
     1321 aatttgctat gggtgctgct gtaaatggta tggctgcaca tggaggttta catccatatg
     1381 gtgcaacatt cttcgtattt agtgattatt taaaaccagc gttacgttta tcatcaatta
     1441 tgggattaaa tgcaacgttc atcttcacac atgattcaat tgcagtaggt gaagatggtc
     1501 ctactcatga accaattgag caattagctg gattaagagc cattccaaat atgaatgtta
     1561 tccgtcctgc tgatggtaat gaaacaagag tagcatggga agttgcctta gaatctgaat
     1621 ctacacctac ttcattagta ttgacacgtc aaaacttacc ggtattagat gtaccagaag
     1681 atgtagttga agaaggcgtt cgaaaaggtg cctatacagt ttatggctct gaagagacac
     1741 cagaattcct attattagct tcaggttcag aagttagtct tgcagttgaa gctgctaaag
     1801 atcttgaaaa acaaggtaaa tcagtacgtg ttgtttcaat gcctaactgg aatgcatttg
     1861 aacaacaatc tgaagaatat aaagaatcag ttattccatc aagcgtaaca aaacgtgttg
     1921 cgattgaaat ggcttcaccg cttggatggc ataaatatgt aggtactgca ggtaaagtta
     1981 ttgctattga cggctttggc gcaagtgcac ctggcgattt agtagttgaa aaatatggat
     2041 ttacaaaaga aaatatctta aaccaagtta tgagcttata agaataattt ataaagcgag
     2101 tatgtttaga agtctaggat gcataatctt aggcttcttt taaagtgttg aaatttagag
     2161 tatagcactt aaactacatc ataagtgata agttatgaaa gtatactatt tcagattaat
     2221 ctttaaaagc tctgttataa cagcatgatt tttgatatta tttttagtat cgatattaaa
     2281 atacttgaat aaactagttc ttgaaataat gtgatgaatt tagtaaaatt cagtaggata
     2341 aagaaagcgg gtgaaacaat ggcaacttgg ttagcaatta tttttatagt agctgcatta
     2401 attttaggtt taattggagg tttcctttta gctagaaaat atatgatgga ctacttgaag
     2461 aaaaacccac caatcaacga agaaatgctt cgtatgatga tgatgcaaat gggtcaaaaa
     2521 ccttctcaga agaaaattaa tcaaatgatg acgatgatga ataaaaatat ggatcaaaat
     2581 atgaagagtg cgaaaaagta aattcgcaat tgatagaggc tattttccag atatggaaat
     2641 ggcctctttt tataatcaaa ttaataagaa taaatatgtt tattaaaatt aaagttaaca
     2701 aaatgacgaa tagactgaga aatgctataa ttcattttgt atgatttaca gagagtttat
     2761 ttaacgagaa ggtgtctgcg tgctctattt aatattttca atcattgtag ctttatttat
     2821 gggaactata gttatagtta ttcgtatgaa agctcaaaat tatccggtaa atgagaaaaa
     2881 aatagttttg ccaccgtttt ttatggcgac cggtgcattg atgtacgtcg ttccatattt
     2941 taggctaaca ggatcggaaa tgctagaagc ctttataatt ggtttgcttt tttctacagt
     3001 tctaatttgg acttctcgat ttgaagtcaa aggtacagaa atttatatga aacgatctaa
     3061 agcatttcca gttattttga tttcattact tatcattcgt actgtgatga aaatattcat
     3121 tagtaatgaa atagatcctg gagaattagg cggcatgttc tttttattag cattctgtat
     3181 gattgttcct tggagagcag caatgctata taaatacaaa aaactaaaga aaacattaat
     3241 caattaatta cttttaaaac cacttgtgat cgacttctaa atcagtcaat gagtggtttt
     3301 aattttactt gaaaaggtga aatgattata ttaaaataaa agcgaacaaa tgttctatat
     3361 ggaggcagta atgaaaatta tacatacagc agactggcac ttagggaaaa tattaaatgg
     3421 caaacagctt ttagaagatc aagcgtatat tttagatatg ttcgtagaaa aaatgaaaga
     3481 agaagaacct gatatcattg tgatagctgg agatttatat gacacaacat atccaagtaa
     3541 agatgcaatc atgttattag aacaagcgat tggaaagtta aatttagaac tgcgtatacc
     3601 aataattatt attagtggaa atcacgatgg taaagagagg ttaaactatg gggcgagttg
     3661 gtttgaacat aatcagttat ttataagaac agattttaca tcgattaatt caccaataga
     3721 gataaatggg gttaattttt atacactccc ttatgctact gtgagcgaaa tgaaacacta
     3781 ctttgaagat gacaccattg aaacacatca acagggaatt acgcgctgta ttgaaacaat
     3841 agcaccggaa attgatgaag atgccgtcaa tattttaatt agtcatctga ctgttcaagg
     3901 tggaaagaca tctgattctg aaagaccatt aactattgga acggttgaat cagttcagaa
     3961 aggtgttttt gatatatttg attatgtcat gctaggtcac ttgcatcatc catttagtat
     4021 agaagacgac aaaattaaat atagtggctc cttattgcag tattcatttt cggaagcggg
     4081 tcaagctaaa gggtatagac gtgtaacaat taatgatggc attattaacg atgtatttat
     4141 tcctcttaag ccacttagac aattggaaat tatctcaggc gaatataatg atgttattaa
     4201 tgaaaaagtt catgtgaaaa ataaagataa ttatttacat tttaaactta aaaatatgtc
     4261 tcatattact gatccaatga tgagtttaaa acaaatttat cctaatactt tagcgctgac
     4321 gaatgaaact tttaattaca atgaagaaaa taatgctata gaaataagtg aaaaagatga
     4381 catgtcaatt atcgaaatgt tttataaaca tataactgat aaagaattat cggatatcca
     4441 atctaaaaag ataaaaaata ttttagaaaa cgaattgaga aaggaggatt aatgaatgaa
     4501 accattacat ttaaagttga ataatttcgg ccccttttta aaagaagaaa ttgatttttc
     4561 taaaattgat aataatgaat tgtttttaat aagtggtaag actggatcgg gtaaaacaat
     4621 gatttttgat gcaatgactt atgccttgtt tggtaaagca tcaactgaac aaagagaaga
     4681 aaatgatttg agaagtcatt tcgctgatgg taaacagccg atgtcagtaa catttgaatt
     4741 tcaattaaat catcgaattt ataaagtgca tagacaaggc ccttatatca aagaaggtaa
     4801 tacaacaaaa acgaacgcta aatttgatgt atttgagatg gtggatggca agtatgaaat
     4861 tagagaaagt aaagtaattt caggtaccca attcattatt gaattattag gagtaaatgc
     4921 agatcaattc cgacaattgt ttattttgcc tcaaggtgaa ttcaaacgct ttttaatatc
     4981 aaacagtcgt gaaaagcaag ggatattaag aacactgttt gacagtgaaa aatttgaagc
     5041 tatacgagaa atattaaaag aagaagtaaa aaaagaaaaa gctcaaatcg agaatagata
     5101 tcaacaaatt gaccttttat ggcaagaaat tgaatcattt gatgatgaca atataaaagg
     5161 cttattagag gttgccactc aacagataga caaattgatt gaaaatatac cacttttaca
     5221 agctaggtcg aaagaaatac tagcatctgt aaatgaaagt aaagaaactg ctattaaaga
     5281 atttgaaata atagaaaaga aaacattaga aaataatata ttaaaagata atattaatca
     5341 actcaacaaa aataaaattg atttcgttca attgaaagaa caacaacctg aaatagaggg
     5401 aattgaagct aagttaaagt tgttacaaga tattacaaac ctattgaatt atattgaaaa
     5461 tagagaaaaa attgaaacta aaattgctaa tagcaaaaaa gatatttcta aaaccaataa
     5521 taaaatattg aatcttgatt gtgataagcg aaacatagac aaagagaaaa aaatgttaga
     5581 agaaaatgga gatttaattg aaagtaaaat ctcttttatt gataaaacta gagtattatt
     5641 taacgatatt aataagtatc aacaaagtta tctcaatatt gaacgcttga gaactgaggg
     5701 tgaacaatta ggtgatgaat taaatgatct aattaaaggt ttagaaacgg tcgaagattc
     5761 aataggtaat aaccaaagtg attacgagaa aattatcgaa ctaaataata cgataacgaa
     5821 cataaataat gaaattaata taattaaaga aaatgaaaaa gctaaagctg aattagataa
     5881 actattaggt agtaagcaag agttagagaa tcaaattaat gaagaaacat ctatattgaa
     5941 gaatctcgaa ataaaattag atcgctacga taaaacaaaa ttggacttaa atgataaaga
     6001 aagctttata agtgaaatta aatctgctgt aaatattgga gatcaatgtc cgatatgtgg
     6061 taatgaaatt caggatttag ggcatcatat tgattttgac agtattgcta aacgtcaaaa
     6121 tgaaattaaa gaaattgaag caaatatcca cgctataaaa tcgaatattg ctgtgcataa
     6181 ttctgaaatt aaatttgtta atgaaaaaat atcgaatatt aatattaaaa cgcaaagtga
     6241 tttttcactt gaagtattga ataagcgtct gctagaaaa
//
LOCUS       DAKTIO010000152         6217 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.154, whole genome shotgun sequence.
ACCESSION   DAKTIO010000152 DAKTIO010000000
VERSION     DAKTIO010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6217
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.154"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..209
                     /locus_tag="O0293_002233"
     CDS             <1..209
                     /locus_tag="O0293_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0281104.1"
                     /translation="NEFNKREVNYSLTQKGKKLSYIHDELHEKSVKKYEEVLKVFDDD
                     EMAVIIEFLNRSIEELKKEDGLND"
     gene            202..1365
                     /locus_tag="O0293_002234"
     CDS             202..1365
                     /locus_tag="O0293_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0281105.1"
                     /translation="MINSNIRLYILGFLAFFASLIQNIYTPIIPRLYDDFQVPLFWIN
                     ATVGGFIFIVAVMQIVLGRSIDSRDSKKVLLTGLGIVIISSFICAVTHNFILFAISRL
                     FQAIGCGIIPLVTLTLLAKLSTDNGRAQAMANYQIFLSCAPALAPILGSTLGARWDYI
                     GIFSFLLVISIVLFLIIFFIDIPNVEKGIVKLTEKIEEKYLTDKVFITLVTLGFLIFF
                     TYFSILVYLPTLLNNTYDIGVGISGVLFLPITVSVILGSLFYKRFSKKYNEIMILRVT
                     ITGFAIFTLLFGWLNESNVIILSVIIFILGTCVGIVPALLSTIISKRFEHIKGKVLGV
                     FNFVRYIGMTVGALLIGIISQPLVAFYFTTITIMLIVIFLYIKIVDFQLKYAK"
     gene            1355..1453
                     /locus_tag="O0293_002235"
     CDS             1355..1453
                     /locus_tag="O0293_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281106.1"
                     /translation="MPNKLKKAVNHFNRWNDSLLFVYRNLKFMPLC"
     gene            complement(1462..2073)
                     /locus_tag="O0293_002236"
     CDS             complement(1462..2073)
                     /locus_tag="O0293_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCY0281107.1"
                     /translation="MEQIITEFISRFGYAAIFILILLENVLPIVPSEIILTFAGLMSV
                     KSHLSILTLFIIATIASFIGLLILYYICRLISEERLYRFIDRHGKWIKLKSKDLKRAN
                     DWFKKYGVWAVFICRFIPVLRVLITIPAGVNRMNVVTFTVISLIGTTIWNFGLILLGR
                     TLSDSFGMLMTGLHTYSRIMYVVIIIAVIYFAIRYIGKRKRVK"
     gene            2620..3897
                     /locus_tag="O0293_002237"
     CDS             2620..3897
                     /locus_tag="O0293_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0281108.1"
                     /translation="MKEKRTNVRWMFALAFFFIGVIAYMDRANISYIAKQMMDDLGMT
                     KPQFGLLASFFSLGYALMQVPSGMLAEKFGPRKMITIALVWWSAFTILTGMIKNHGLI
                     YLVRFLFGVGEAPMYPSNAVFNSFWFSKNEKGRASSALLAGSYFGPVLAPIVTIAIVN
                     AFNWQAVFYIFGAVGILMAVLWAIIAKDLPEQHRMVNEAEKRFIMENRDIVATEKSSP
                     PWNDFFKRFSFYAIAIQYFVVQFIITLFLIWLPTYLTEVFHVNFKEMSISSLPWLLMF
                     FLILSAGAISDRVLGLGRSKFVARGVIAIAGFIVFAVSIIFAVRTGNLYVSIFWLSLG
                     LGGIGISMGMSWAAATDLGRNFSGTVSGWMNLWGNIGALISPLLAGLFVEHLGWTMTF
                     QLLIVPAVIAVIMWFYVKPDQPLIVSDDKAIEK"
     gene            4375..6198
                     /locus_tag="O0293_002238"
     CDS             4375..6198
                     /locus_tag="O0293_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCY0281109.1"
                     /translation="MIIPAIIGYLCGNFQFGLLVATGTLAHIYVFKGPSRSKLRTVII
                     CNLAFAICMMLGTLTAKTPLVFGMTLLIVTVIPFYIFTALKIAGPSSTFFIVTFSLPI
                     NLPIAPEEALYRGFAILVGGILATMMVLITIVFSKNKAEEQAIQNDFKLISKLLHTYN
                     DKSAFLQVAKTAVDSFKASDKLLITSTSSNDKLSRRFQKLLLLHTSAQGIYSELLELN
                     AKQIRPLPDELIEMMDHIIAQLDNSEENVRYWRKEVTVTEEFQNLFNHILKIDEMVHA
                     NEARIAYEADMRKPLYSKRIYQNLTLDSIVFRNTLRYTAIMMIAIFIALMFDFEKAYW
                     IPLSAHTILLGTSTIHAIERGMARGLGTILGVLVLSVILLFSIPTPVAVILMGIAALF
                     TEALVGANYAIAVVFITIQVILMNGLASQNLTINIAFPRVIDVAMGIVIAIIGLFVLG
                     QRTASALLPNVMAEVVRKEATLFHYLFSENQYKDDVYQKNTAMNLSVKLNNMTQVYNA
                     ANGELFSDKTLIQNYYPSLFALEEISFMLNRAMANEDRLTINEQLMGEYLAIFENIAK
                     HFELNTELEIKILPDLPQYNYIQSAMMNIQHNGFRERDKNV"
BASE COUNT         2148 a          853 c         1099 g         2117 t
ORIGIN      
        1 acaatgaatt taacaaaaga gaagttaatt attcattaac acaaaaagga aaaaaattat
       61 cttatataca tgatgaatta catgaaaaat cggtaaaaaa atatgaagag gtacttaaag
      121 tctttgatga tgatgaaatg gcagttatta tagagttttt aaaccgtagt atagaagaat
      181 taaaaaaaga agatggttta aatgattaat agtaatatca gattatatat tctaggattt
      241 ttagcatttt tcgcatcatt gattcaaaac atatacacgc ctattattcc gagactatat
      301 gacgattttc aagtaccact attttggata aatgcgacag ttggcggttt tatatttatc
      361 gttgcagtta tgcaaattgt acttggtaga agtatagatt ctcgtgattc gaaaaaagta
      421 cttttaacgg gattgggaat agtaatcata tctagcttta tttgtgcagt gacacataac
      481 tttatattat ttgctatatc aaggttattt caagcaattg gttgcggaat tattcctctc
      541 gtgactttaa ctttattagc caaactaagt acagataatg gtagagctca agctatggcg
      601 aactatcaaa tatttttatc atgtgcacca gctttggcac caatcttagg gagtacttta
      661 ggtgcaagat gggattatat tggtattttt agttttttgc tcgttatatc tatcgtatta
      721 tttttgatta tcttttttat tgatattcca aatgttgaaa aagggattgt aaaattaact
      781 gaaaaaattg aagaaaaata tttgaccgat aaagtgttta ttactttagt gacattggga
      841 tttttaatat tttttacgta cttttcaata ttagtttatt taccaacatt attaaataat
      901 acctatgata taggcgttgg aatatcagga gttttgtttt tacctattac tgttagtgtt
      961 atattaggaa gtttatttta taagcgattt tcaaaaaaat ataatgaaat aatgatactt
     1021 cgtgttacta taactggatt tgctatattt acgttattgt ttggatggtt aaacgaatca
     1081 aatgtaatta tattatcagt aattatattt atattaggta cttgtgttgg tattgtgcca
     1141 gctttgctgt ctactataat ttctaaaagg tttgaacata taaaagggaa agtgctaggt
     1201 gtatttaatt ttgtgagata tattggaatg actgtcggtg cattattaat tggtatcatt
     1261 tctcagccgt tggtagcctt ttacttcaca actataacta tcatgttaat agtaatattt
     1321 ctttatataa agatagttga ctttcagcta aagtatgcca aataaactta aaaaagcagt
     1381 gaatcatttc aatagatgga atgattcact gctttttgtt tatagaaatt taaaattcat
     1441 gcctttatgc taacaacaat attattttac tctcttacgt ttgccaatgt aacgtatagc
     1501 aaagtaaatg actgcgataa tgatgacaac atacataata cgtgaatatg tatgaagacc
     1561 agtcatcaac ataccaaagc tatcgctcaa agtgcgtcct agtaaaatta aaccgaaatt
     1621 ccagattgta gtacctatta atgaaataac ggtaaatgtc acaacattca tgcggtttac
     1681 accagctgga atggtaatca atacacgtaa tacaggtatg aaacgacaga taaatacagc
     1741 ccatacgcca tactttttaa accaatcatt tgctcgcttt aaatccttac ttttcaattt
     1801 aatccactta ccgtgtcgat caataaaacg atatagacgt tcttctgaaa tcaaacggca
     1861 gatataatat aaaattaaca gccctataaa cgatgcgata gttgcaataa taaataaagt
     1921 taaaattgat aaatgtgatt taacagacat aagaccagca aatgtcagaa taatttccga
     1981 tggtacgata ggtagtacat tttctaataa aattaatata aaaatggctg catacccaaa
     2041 acggctaata aattcagtga taatttgttc cattattaaa aataagctcc tttaattttc
     2101 agcaattgca gacaaataga aactggataa attcaaatga ataattaatg atgtcgcatg
     2161 cgatgttgaa acaaacattt gtcttaaaat gtattataag caataaatca aaaaattgct
     2221 tgctataaga gcaaaaagtc aataaatgat catctaaaca acaggcgctg cacaatgaat
     2281 ggttaatgta gttatatgca atttaaaatg aatgtcgaat atgtaagaaa gcctgcaata
     2341 tacgttttaa aacactagga ttgcagtatt tttaattaac aaacgatagg ctactatgta
     2401 actaaaagta cctttatatc gttttaatag caaaagttaa gcggaattta ataaaatttt
     2461 tatagattct attgtaaacg ctttccatgt ctgataatat tataaaaaat aactttggta
     2521 caattttgtg acaaagtagg ggtggacatg ccagatgtcc gaaaatttta acgtttcagg
     2581 gtaagtttta aaaaattaat tagggaagta ggaatagtta tgaaggaaaa acgaactaac
     2641 gtaaggtgga tgttcgcgct tgcgttcttc tttattgggg ttattgcgta tatggataga
     2701 gcaaacattt catatatcgc taagcaaatg atggatgatt tagggatgac aaaaccacaa
     2761 tttggtttat tggcatcatt cttctctcta ggttatgcat taatgcaagt accatcgggg
     2821 atgttggctg aaaaattcgg tccacgtaag atgattacaa ttgcattagt ttggtggagt
     2881 gcatttacaa tcttaacggg tatgattaag aaccacggtt taatttattt agtgagattc
     2941 ttatttggtg taggtgaggc gccaatgtac ccttctaatg ctgtgtttaa ttcattttgg
     3001 ttctctaaaa atgaaaaagg tagagcatca agtgcattat tagcaggatc atatttcgga
     3061 cctgtattag caccaatagt tacaattgct attgttaacg catttaactg gcaagcagta
     3121 ttttacattt ttggtgcagt aggtatttta atggctgtat tatgggcgat tattgccaaa
     3181 gacttacctg agcaacatag aatggttaat gaagcggaga aacgtttcat tatggaaaat
     3241 cgtgatatcg tagctactga aaagtcatca ccaccatgga atgatttctt taaacgtttt
     3301 agcttctatg caattgcaat tcaatacttt gttgtacaat ttatcattac attattctta
     3361 atttggttac cgacgtattt aacagaagta ttccacgtta actttaaaga aatgagcatt
     3421 agttcattac cttggttatt aatgttcttc ttaatcttat cagcaggtgc aatttctgac
     3481 cgtgtattag gattaggtcg ttcaaaattc gtagctagag gtgtaattgc aattgcagga
     3541 tttattgtgt ttgcagtttc aattatcttt gctgtacgca caggaaattt atatgtaagt
     3601 attttctggt tatcactagg tcttggtggt atcggtattt caatgggtat gagttgggct
     3661 gcagcaactg acttaggacg taacttctct ggtacagtat cagggtggat gaacttatgg
     3721 ggtaatatag gtgcattaat cagtccgcta ttagcaggtc tattcgtaga acatttgggt
     3781 tggacaatga cattccaatt gttaatcgtt ccagcagtaa tcgctgtgat tatgtggttc
     3841 tatgtgaaac cagatcaacc tttaattgtt agtgatgata aagcaataga aaaataattt
     3901 aaacaagcag taagctttca catagttggg gcttattgct ttttttgcgt tgaaattgaa
     3961 actttttaaa acagatatgg tttaagatga aaatgaagtt attgaaatga tatatgtaaa
     4021 gaaataaggt tttaaaacat tagtcaggta acgcttgtaa aagtacatat aaattttaac
     4081 tagcgcaaag gtgggcgacc aaaggttcaa cgatgttaaa taacattaga aattaatttt
     4141 aattggactt taaaagtttt taaatttaga taattgagca taaggtgtta taatgacata
     4201 tgttgcgtaa ttaaaattta tagcaacaaa ttcattttaa ctatgctaat aaaaagatta
     4261 tggaaatatt ttgacaagga aaggagaagt cgaaatgaca tctttttgac atcactcata
     4321 aaaatcaatc gacttaactt agacttttat aaaggtgtaa gacagggact gttaatgatt
     4381 attcctgcaa taatcggtta cttatgtggt aatttccaat ttggattatt agttgcaacc
     4441 ggaacactag cccatattta tgtttttaaa ggtccgtcgc gatctaagct gcgaactgta
     4501 ataatttgta atttagcgtt tgcaatatgt atgatgcttg gtacgctaac agccaaaacg
     4561 ccactcgttt ttggaatgac attattaatt gttacggtta taccatttta tatatttact
     4621 gccttaaaaa tagctggacc gtcatcgaca ttcttcattg tgacattcag tctacccatt
     4681 aacttaccta tagctcccga agaagcatta tatagaggct ttgcgatttt agtaggcggt
     4741 atacttgcca ctatgatggt gttaatcacg atcgtatttt ctaaaaacaa agctgaagaa
     4801 caagcaattc aaaatgattt taaactcata tctaagttgt tacacactta taatgataaa
     4861 tctgcttttt tacaagtagc aaaaacagcg gtggatagtt ttaaagcatc tgataaatta
     4921 ttaatcactt ctacttcaag taacgataaa ttaagtagac gtttccaaaa attattatta
     4981 ttacacacat ctgcccaagg gatttattct gaactgttag agttgaacgc taaacaaatt
     5041 cgaccattgc cagatgagtt aattgaaatg atggatcata tcattgcaca actagataat
     5101 agtgaggaaa atgtaagata ttggcgaaaa gaagtgacag taacagagga atttcaaaat
     5161 ttattcaacc atatattgaa aattgatgaa atggtgcatg caaatgaagc gcgtattgcg
     5221 tatgaagcag acatgcgaaa acctttatat agtaaacgca tttatcaaaa tttaacatta
     5281 gactctattg tttttagaaa tacattgaga tatacagcga ttatgatgat agcgatattt
     5341 attgcgttaa tgtttgattt tgaaaaagca tactggatac cgttatctgc acatacaata
     5401 ttactaggaa catcaactat acatgcaatc gagagaggta tggcacgagg tttaggtact
     5461 attttaggtg tgttagtact ttcagtcata ttgttgtttt caataccaac acctgttgca
     5521 gtaattttaa tgggcattgc agcattgttt actgaagcat tggtgggagc aaattatgcg
     5581 attgcagtag tttttattac aatacaagtt attttaatga acggattagc atcacagaat
     5641 ttaacaatta acattgcgtt tccaagagtt attgacgttg caatgggtat tgtgattgca
     5701 atcataggtt tatttgtcct tggacaacgt accgcatccg cattgcttcc taatgtaatg
     5761 gctgaagttg ttcgtaaaga agcaacgctc tttcattatt tattttctga aaatcaatat
     5821 aaagatgatg tgtatcagaa aaacacggct atgaacctat ctgtgaaatt aaataacatg
     5881 acgcaagttt ataatgcagc taatggtgaa ttatttagtg ataagacatt gattcaaaat
     5941 tattatccta gcttattcgc attagaagaa attagcttta tgttaaatag agccatggct
     6001 aatgaagata gacttacaat aaatgaacaa ttaatgggcg agtatttagc aatatttgaa
     6061 aacatcgcta agcatttcga attaaataca gaattagaaa taaagatatt accagattta
     6121 ccacagtata attatattca atcagcaatg atgaatattc aacacaatgg ttttcgtgag
     6181 agagacaaga acgtttaaaa ttaaatattt atcaggt
//
LOCUS       DAKTIO010000153         6217 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.155, whole genome shotgun sequence.
ACCESSION   DAKTIO010000153 DAKTIO010000000
VERSION     DAKTIO010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6217
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.155"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            91..492
                     /locus_tag="O0293_002239"
     CDS             91..492
                     /locus_tag="O0293_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0281110.1"
                     /translation="MKKVNNDTVFGILQLETLLGDINSIFSEIESEYKMSREEILILL
                     TLWQKGSMTLKEMDRFVEVKPYKRTRTYNNLVELEWIYKERPVDDERTVIIHFNEKLQ
                     QEKVELLNFISDAIASRATAMQNSLNAIIAV"
     gene            complement(612..1175)
                     /locus_tag="O0293_002240"
     CDS             complement(612..1175)
                     /locus_tag="O0293_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0281111.1"
                     /translation="MKLERILPFSKTLIKQHITPESIVVDATCGNGNDTLFLAEQVPE
                     GHVYGFDIQDLALENTRDKVKDFNHVSLIKDGHENIEHHINDAHKGHIDAAIFNLGYL
                     PKGDKSIVTKPDTTIQAINSLLSLMSIEGIIVLVIYHGHSEGQIEKHALLDYLSTLDQ
                     KHAQVLQYQFLNQRNHAPFICAIEKIS"
     gene            complement(1172..2125)
                     /locus_tag="O0293_002241"
     CDS             complement(1172..2125)
                     /locus_tag="O0293_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500380.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCY0281112.1"
                     /translation="MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRD
                     GTVAHGGCTFCSAAGSGDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTH
                     APVEVLKEKFEPVLKEPGVVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIH
                     QSTSDLINRAHDMKTYYDGVAKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDV
                     QGIKIHLLHLLKGTPMVKQYDKGLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGP
                     IDIMVGPMWSVNKWEVLNGIDAELARRNSYQGLRYKSKVKQ"
     gene            2235..3416
                     /locus_tag="O0293_002242"
     CDS             2235..3416
                     /locus_tag="O0293_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0281113.1"
                     /translation="MNIPKSVWWLVIGMALNITGSSFLWPLNTIYMKQELGKSLTVAG
                     LVLMINSFGMVIGNLLGGSLFDKLGGYKTILIGTFTCLCSTTLLNFFHGWPWYAVWLV
                     MLGFGGGMIIPAIYAMAGAVWPNGGRQTFNAIYLAQNIGVAVGAAMGGFVAEFSFNYI
                     FLANLIMYVVFALVAVTQFNIEINAKVKYPTHLDITGKKNKARFISLVLICAMFAICW
                     VAYIQWESTIASFTQSINISMAQYSVLWTINGIMILVAQPLIKPILYLLKGNLKKQMF
                     VGIIIFMLSFFVTSFAENFTIFVVGMIILTFGEMFVWPAVPTIANQLAPDGKQGQYQG
                     FVNSAATVGKAFGPFLGGVLVDAFNMRMMFIGMMLLLVFALILLMVFKENNTQPKKID
                     A"
     gene            3704..6121
                     /locus_tag="O0293_002243"
     CDS             3704..6121
                     /locus_tag="O0293_002243"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCY0281114.1"
                     /translation="MLNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPS
                     GAGLHVGHPEGYTATDIISRYKRMQGYNVLHPMGWDAFGLPAEQYALDTGNDPREFTK
                     KNIQTFKRQIKELGFSYDWDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCP
                     ALGTVLSNEEVIDGVSERGGHPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDM
                     QRNWIGRSEGAKVSFDVDNTEGKVEVFTTRPDTIYGASFLVLSPEHALVNSITTDEYK
                     EKVKAYQTEASKKSDLERTDLAKDKSGVFTGAYATNPLSGEKVQIWIADYVLSTYGTG
                     AIMAVPAHDDRDYEFAKKFDLPIIEVIEGGNVEEAAYTGEGKHINSGELDGLENEAAI
                     TKAIQLLEQKGAGEKKVNYKLRDWLFSRQRYWGEPIPVIHWEDGTMTTVPEEELPLLL
                     PETDEIKPSGTGESPLANIDSFVNVVDEKTGMKGRRETNTMPQWAGSCWYYLRYIDPK
                     NENMLADPEKLKHWLPVDLYIGGVEHAVLHLLYARFWHKVLYDLAIVPTKEPFQKLFN
                     QGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLRLYEMFMGPLDAAIAWSEKGLDGS
                     RRFLDRVWRLMVNEDGTLSSKIVTTNNKSLDKVYNQTVKKVTEDFETLGFNTAISQLM
                     VFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSKLGHEESITYQPWPTYDEALL
                     VDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVKASIEGKDIMKVIAVPQK
                     LVNIVAK"
     gene            6143..>6217
                     /locus_tag="O0293_002244"
     CDS             6143..>6217
                     /locus_tag="O0293_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCY0281115.1"
                     /translation="MKSITTDELKNKLLESKPVQIVDVR"
BASE COUNT         2079 a          905 c         1197 g         2036 t
ORIGIN      
        1 ttagcacata caagttttgg gattgttggg atgtttgtta atacttgtat agtagctaaa
       61 tatgtgatta ttaattggga gatgtttagc atgaaaaaag taaataacga cactgtattt
      121 ggaattttgc aattagaaac acttttgggt gacattaact caattttcag cgagattgaa
      181 agcgaataca aaatgtctag agaagaaatt ttaattttac taactttatg gcaaaaaggt
      241 tctatgacgc ttaaagaaat ggacagattt gttgaagtta aaccgtataa gcgtacgaga
      301 acgtataata atttagttga attagaatgg atttacaaag agcgtcctgt tgacgatgaa
      361 agaacagtta ttattcattt caatgaaaag ttacaacaag agaaagtaga gttgttgaat
      421 ttcatcagtg atgcgattgc aagtagagca acagcaatgc aaaatagttt aaacgcaatt
      481 attgctgtgt aagtttaata gcataaaaag aggttttcat taagttgaaa acctcttttt
      541 gttgttggca ttaatttttc aaatgttgac tactcaatcc taaattataa atagtatagc
      601 gcagcaaatg cttaagaaat tttttctatg gcacaaatga atggagcatg attacgttgg
      661 tttaaaaatt gatattgcaa aacttgcgca tgcttttgat ccaaagtact caagtaatca
      721 agcaatgcat gcttctcaat ttgtccttcg ctatgaccat gatatataac aagtacaata
      781 ataccttcaa ttgacattaa tgatagcaat gaattaatag cttggattgt cgtgtcaggc
      841 tttgtcacga tagatttatc acctttaggc aaataaccta ggttaaagat ggctgcatca
      901 atatgacctt tatgtgcatc atttatatga tgttcaatat tttcatgtcc atcttttatt
      961 aaagaaacat gattgaaatc cttaacttta tcacgtgtat tttccaaagc taaatcttga
     1021 atgtcgaaac cataaacatg tccttctggt acttgttcgg ctaaaaataa agtgtcattg
     1081 ccgttaccgc aagttgcgtc tacaacaata ctttctggtg ttatatgttg tttaataagt
     1141 gtttttgaaa aagggagtat acgttctaat ttcattgctt caccttagac ttgtaacgca
     1201 agccttgata agaatttcta cgtgctaatt cagcatcgat gccatttaat acttcccatt
     1261 tattaacact ccacattgga cctaccatga tatctattgg accatcaccg gtaattcggt
     1321 gaacgatcat ttcaggggga atcacttcta attggtccac aactaggttt gtgtactctt
     1381 cttgagtcat aaaagttaat aaacctttat cgtattgttt taccatcggt gtacctttta
     1441 acaaatgaag taaatgaatt ttaatacctt gtacatccat ttgtgcaact tctttggcag
     1501 tagccatcat catgtcatag tcttcgccag gtaagccatt aatgatgtgt gtacatacat
     1561 tgatattatg cttacgtaat tttgccacac catcataata agttttcata tcatgggcac
     1621 gattgattaa atcagatgtt gactgatgga ttgtttgtag tcctaattca acccataagt
     1681 atgttcgttg attcaaatct gctaaatatt cgacaacatc gtctggtaga cagtcaggac
     1741 gcgtaccaat agataatccc acaacacccg gttctttaag tacaggttcg aatttttctt
     1801 ttaatacttc aaccggtgca tgtgtatttg taaatgcctg aaaataagca atatattttc
     1861 cttcgtgcca tttctcatgc atcttttctt taatttcttt aaattgtact gcgattgaat
     1921 ctgcacgatt acctgcaaag tctccgctac ctgcagcaga acaaaatgta catccaccat
     1981 gtgctacagt gccatcgcgg ttaggacagt caaacccgcc atccaatgca actttaaata
     2041 ttttttgtcc aaatttattt tttaaatggt aattccatgt gtgataacgt ttgttttcaa
     2101 aagcgtattg gaaatgattg cccatatgtc attttccttt ctataaaaaa agagttctaa
     2161 gtacagattt taacatattt taatgttata gtgtttatta tagtttgaca aaaaagagag
     2221 aggaactatg aaatatgaat atacctaaat cagtctggtg gctagtaatt ggcatggcgt
     2281 taaatattac tggttccagt tttttgtggc ctttaaatac aatttatatg aaacaagaac
     2341 ttggaaaaag tttaactgtt gctggtttag tgctaatgat aaattcattt ggcatggtta
     2401 ttggaaactt attaggtggt tcactatttg ataaattagg tggatacaag acgattttaa
     2461 ttggaacttt cacttgtctt tgtagtacaa cgctacttaa tttctttcac gggtggcctt
     2521 ggtatgctgt atggcttgta atgttagggt ttggtggcgg aatgattatt cctgcgatat
     2581 acgctatggc tggagcagtg tggccaaatg gcggaagaca aacgtttaat gcgatatact
     2641 tagcgcaaaa tattggtgtg gctgtcggtg ctgcaatggg cggctttgtc gcagaattta
     2701 gctttaacta tatcttttta gccaatctta ttatgtatgt tgtgtttgcg cttgtcgcgg
     2761 taacgcaatt taatattgaa attaatgcga aagttaaata tccaactcat ttagatatta
     2821 ctggtaaaaa gaataaagca agatttattt cattagtact aatttgtgca atgtttgcaa
     2881 tttgttgggt tgcatatatt caatgggagt ctacaatcgc ttcatttaca caatctatta
     2941 atatttcaat ggcacaatat agtgttttat ggacaattaa cggaataatg attttagtag
     3001 cacaaccatt aattaaaccg attctctatc tgttaaaagg aaacttaaag aagcaaatgt
     3061 ttgtcggcat catcattttt atgttgtcgt tctttgtcac gagttttgcc gaaaacttta
     3121 caatatttgt tgtcggtatg attattttaa cttttggaga aatgtttgta tggccagcag
     3181 ttccaactat agccaatcag ttagcgccag atggtaagca aggacagtac caaggttttg
     3241 tgaattcagc tgctacagta ggaaaagcat ttggtccatt tcttggtggt gtattagttg
     3301 atgcgtttaa tatgcgcatg atgtttatcg gtatgatgct actacttgta tttgcattaa
     3361 tattattaat ggttttcaag gagaataata cgcaacctaa aaaaatagat gcataatgag
     3421 taaatagaat taacgttata gacttgaaat aaatgtcgtt ataacataat attaatttgt
     3481 ataatttaat ttcgtttgga gcttttctac agaaagctag tgatgctgag agctagtgtt
     3541 aaggactaaa tgtaaatcgt attaatttta aattgaatga atgacatctc ttactattaa
     3601 aatgagtgca caatttttgt gaaatagggt ggtaacgcgg caaatgtcgt ccctatgtaa
     3661 atagaatagt tagaggtgtc ttttttattg aataggagga aatgtgttga attataacca
     3721 caatcaaatt gaaaagaaat ggcaagacta ttgggacgaa aataaaacat ttaaaacaaa
     3781 tgataactta ggtcaaaaga aattttatgc tttagacatg tttccatatc catcaggtgc
     3841 tggtttacat gttggacatc ctgagggcta tacagcaaca gatatcattt caagatataa
     3901 aagaatgcaa ggatataatg tattacatcc gatggggtgg gatgcattcg gattaccagc
     3961 agagcaatat gctttagaca ctggcaacga cccacgtgaa tttacaaaga aaaatatcca
     4021 aacttttaaa cgacaaatta aagaattagg gttcagttat gattgggatc gtgaagttaa
     4081 tacaacagat ccagaatact ataaatggac acagtggatt ttcatacagt tatataacaa
     4141 aggtttagca tacgttgatg aagttgcagt taactggtgt ccagcattag gcactgtttt
     4201 atctaacgaa gaagtgattg atggtgtctc tgaacgtggt ggacatccag tttatcgtaa
     4261 gccgatgaaa caatgggtac ttaaaatcac agaatatgca gatcaattat tagcagattt
     4321 agatgattta gattggcctg agtctttaaa agatatgcag cgcaattgga ttggacgttc
     4381 tgaaggggcc aaagtttcat ttgatgtaga taatacggaa ggaaaagtag aagtatttac
     4441 gactagacca gatacaatct atggtgcatc attcttagtc ttaagtcctg aacatgcatt
     4501 agttaattca attacaacag atgaatataa agaaaaagta aaagcttatc aaacagaagc
     4561 ttctaaaaag tcagatttag aacgtacaga tttagcaaaa gataaatcag gtgtatttac
     4621 tggtgcatat gcaactaatc ctttatctgg tgaaaaagta caaatttgga ttgctgatta
     4681 tgtattatca acatatggta ctggagcaat tatggcagta ccagcgcatg atgacagaga
     4741 ttatgaattt gctaaaaagt ttgatttgcc aatcattgaa gtcatcgaag gtggaaatgt
     4801 tgaagaagca gcatacactg gtgaaggtaa acatattaat tctggtgaac ttgatggttt
     4861 agaaaatgaa gcggcaatta ctaaagctat tcaattatta gagcaaaaag gtgctggcga
     4921 aaagaaagtt aattacaaat taagagattg gttattcagt cgtcagcgtt attggggcga
     4981 accaattcct gtcattcatt gggaagatgg aacaatgaca actgttcctg aagaagagct
     5041 accattgttg ttacctgaaa cagatgaaat caagccatca gggactggtg agtctccact
     5101 agctaatatt gattcatttg taaatgttgt agatgaaaaa acaggtatga aaggacgtcg
     5161 tgaaacaaat acaatgccac aatgggcagg tagttgttgg tattatttac gttacatcga
     5221 tcctaaaaat gaaaatatgt tagcagatcc tgaaaaatta aaacattggt tacctgttga
     5281 tttatatatc ggtggagtag aacatgcggt tcttcactta ttatatgcaa gattttggca
     5341 taaagtcctt tatgatttgg ctatcgtacc tactaaagaa cctttccaaa aattatttaa
     5401 ccaaggtatg attttaggag aaggtaatga gaagatgagt aaatctaaag gaaatgtaat
     5461 caatcctgat gatatagtac agtctcatgg tgcagatact ttgcgtcttt acgaaatgtt
     5521 tatgggacct ttagatgctg caattgcatg gagtgaaaaa ggattagatg ggtctcgtcg
     5581 attcttagat cgcgtatggc gtttaatggt aaatgaagat gggacattga gttcaaaaat
     5641 tgtaactaca aataataaat ctttagataa agtttataac caaactgtta aaaaggtaac
     5701 agaagacttt gaaacattag gatttaatac tgctattagt caattaatgg tatttattaa
     5761 tgagtgttat aaagttgatg aagtttataa accttacatt gaaggcttcg ttaaaatgtt
     5821 agcacctatt gcaccacata tcggtgaaga attatggtca aaattaggac atgaagagtc
     5881 tattacgtac caaccttggc caacttatga cgaagcacta cttgtagatg atgaagtaga
     5941 aatcgttgtt caagtgaatg gtaaattgag agctaaaatt aaaattgcta aagatacatc
     6001 aaaagaagaa atgcaagaaa ttgccttatc taatgacaat gttaaagcga gtattgaagg
     6061 taaagacatc atgaaagtca tcgctgttcc tcaaaaatta gtcaatattg tagctaaata
     6121 atgttttaag gaggactttg aaatgaagtc aattactaca gatgaattaa aaaataaact
     6181 tttagaatct aaaccagttc aaattgttga tgttcgt
//
LOCUS       DAKTIO010000154         6201 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.156, whole genome shotgun sequence.
ACCESSION   DAKTIO010000154 DAKTIO010000000
VERSION     DAKTIO010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6201
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.156"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..764
                     /gene="ftsW"
                     /locus_tag="O0293_002245"
     CDS             <1..764
                     /gene="ftsW"
                     /locus_tag="O0293_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCY0281116.1"
                     /translation="SKPKLILSPIVLALGCTFLVFLQKDVGQTLLILIILVAIIFYSG
                     IGVNKVLRFGIPAVLGFLVVFVIALMAGWLPSYLTARFSTLTDPFQFESGTGYHISNS
                     LLAIGNGGVFGKGLGNSAMKLGYLPEPHTDFIFAIICEELGLIGGLLVITLEFFIVYR
                     AFQFANKTSSYFYKLVCVGIATYFGSQTFVNIGGISATIPLTGVPLPFISFGGSSMIS
                     LSIAMGLLLIVGKQIKVDQQRKKQQQKVDIRRQFN"
     gene            1318..4770
                     /locus_tag="O0293_002246"
     CDS             1318..4770
                     /locus_tag="O0293_002246"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HCY0281117.1"
                     /translation="MKQIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHR
                     YKADESYLVGSDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEG
                     IKFIGPHLEHLDMFGDKVKARTTAIKADLPVIPGTDGPIKSYELAKEFAEEAGFPLMI
                     KATSGGGGKGMRIVREESELEDAFHRAKSEAEKSFGNSEVYIERYIDNPKHIEVQVIG
                     DEHGNIVHLFERDCSVQRRHQKVVEVAPSVGLSSTLRQRICDAAIQLMENIKYVNAGT
                     VEFLVSGDEFFFIEVNPRVQVEHTITEMVTGIDIVKTQILVAAGADLFGEEINMPQQK
                     DITTLGYAIQCRITTEDPLNDFMPDTGTIIAYRSSGGFGVRLDAGDGFQGAEISPYYD
                     SLLVKLSTHAISFKQAEEKMVRSLREMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFI
                     EETPELFNIQPSLDRGTKTLEYIGNVTINGFPNVEKRPKPDYELASIPTVSSSKIASF
                     SGTKQLLDEVGPKGVAEWVKKQDDVLLTDTTFRDAHQSLLATRVRTKDMINIASKTAD
                     VFKDGFSLEMWGGATFDVAYNFLKENPWERLERLRKAIPNVLFQMLLRASNAVGYKNY
                     PDNVIHKFVQESAKAGIDVFRIFDSLNWVDQMKVANEAVQEAGKISEGTICYTGDILN
                     PERSNIYTLEYYVKLAKELEREGFHILAIKDMAGLLKPKAAYELIGELKSAVDLPIHL
                     HTHDTSGNGLLTYKQAIDAGVDIIDTAVASMSGLTSQPSANSLYYALNGFPRHLRTDI
                     EGMESLSHYWSTVRTYYSDFESDIKSPNTEIYQHEMPGGQYSNLSQQAKSLGLGERFD
                     EVKDMYRRVNFLFGDIVKVTPSSKVVGDMALYMVQNDLDEQSVITDGYKLDFPESVVS
                     FFKGEIGQPVNGFNKDLQAVILKGQEALTARPGEYLEPVDFEKVRELLEEEQQGPVTE
                     QDIISYVLYPKVYEQYIQTRNQYGNLSLLDTPTFFFGMRNGETVEIEIDKGKRLIIKL
                     ETISEPDENGNRTIYYAMNGQARRIYIKDENVHTNANVKPKADKSNPSHIGAQMPGSV
                     TEVKVSVGETVKANQPLLITEAMKMETTIQAPFDGVIKQVTVNNGDTIATGDLLIEIE
                     KATD"
     gene            complement(4910..5821)
                     /locus_tag="O0293_002247"
     CDS             complement(4910..5821)
                     /locus_tag="O0293_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HCY0281118.1"
                     /translation="MFGKKNLKWLGVVATLMMTFVQLGGALVTKTGSADGCGSSWPLC
                     HGALIPEFFPIDTIIELSHRAVSALSLLMVLWLVITAWKHIGYIKEIKPLSIISVGFL
                     LLQALIGAAAVIWQQNDYVLALHFGISLISFSSVFLITLIIFSIDQKYEADELYIKKP
                     LRRLTWLMAIIIYCGVYTGALVRHADASLAYGGWPLPFHDLVPHSEQDWVQLTHRIMA
                     FIVFTIIMITYIHAVKNYPNNRTVHYGYTAAFILVILQVITGALSIMTNVNLIIALFH
                     ALFITYLFGMTTYFIMLMLRSVRSDKQ"
BASE COUNT         2197 a          929 c         1144 g         1931 t
ORIGIN      
        1 taagtaaacc aaaattaatt ttaagtccta ttgtattagc attaggttgt acgtttttag
       61 ttttcctaca aaaagacgta gggcaaacat tactaatatt aattatttta gttgcgatca
      121 ttttttattc aggaattggg gtaaacaaag tcctaagatt tggtatacca gcagtgctag
      181 gattcttagt agtatttgtc attgcattaa tggctggttg gttaccaagt tatttaactg
      241 ccagatttag tacgctaaca gatccattcc aattcgaatc aggaactgga taccatattt
      301 ccaattcatt gcttgcgata ggtaacggtg gcgtatttgg aaaaggatta ggaaatagtg
      361 caatgaaatt gggctattta ccagaaccac atacagattt tatttttgca attatttgcg
      421 aagaattagg tttaatcgga ggattgctag ttattacttt agagttcttt attgtatatc
      481 gtgccttcca gtttgcaaat aaaacatcat catattttta taaacttgtg tgtgttggga
      541 ttgccacata ctttggaagt caaacgtttg taaacattgg cggtatttcg gcaacaattc
      601 cattaactgg tgtgccattg ccatttatca gctttggtgg atcatcaatg attagtttaa
      661 gtattgctat gggtttactt ctgattgtag gtaaacaaat caaagtagac cagcaacgaa
      721 agaaacaaca acaaaaagtt gatataagaa gacaatttaa ttaaaaaaat actagccaat
      781 atttagtacg agatgacttc tgtcattaag caatgctaaa cattggctat ttatatactc
      841 tttgtcaaat tggactgatg agttcaataa cggaagttag gcaaccaaac aacgcttaac
      901 ttcctttttg ttttgatatt acgagattac tcatgattat caaattttat aaacaaaaca
      961 acgtataatt ataactaaat tttatgacta aaaattacta aatgtggtgg cgaaattaga
     1021 atcatgatga taagataaag ttatcgctat tgataataaa tgttgatgtt ttgcaatttt
     1081 atttttaatt ttttagagtt gtagaaatcg aaaatgtttc acaacaaaaa cgtgaactac
     1141 ctgttgttgc atagtcattt ttgaaattat tatttctgaa gcggaggttt taattgtctc
     1201 aagaatgtta aattttgaaa atttgaaaca taactatagc aaaacagagc gctttaagat
     1261 aaaattttat tatctaatta gttagaaaat tctaatagtt tgaggagtgt atcgctattg
     1321 aaacaaataa aaaagttact tgttgctaac cgtggagaaa ttgcaattcg tatattcaga
     1381 gcggcggcag aattagacat cagcacagtt gcaatttatt cgaatgaaga caaaagttca
     1441 ttacatagat ataaagcaga tgaatcctat ttagttggaa gtgatttagg tcctgctgaa
     1501 agttatttaa atattgagcg tatcattgat gtagcaaaac aagcgaatgt ggatgcgatt
     1561 catcctggct atggattttt aagtgaaaat gaacaatttg cgcgtcgttg tgcagaagaa
     1621 ggaattaaat ttattggtcc tcatttagaa catttagata tgtttggaga taaagttaaa
     1681 gctcgtacaa cggctatcaa ggcagattta ccagttattc ctggtacaga cggtccaatt
     1741 aaatcatatg aattagcaaa agaatttgca gaagaagctg gtttcccgct aatgattaaa
     1801 gccacaagtg gtggcggcgg taaaggtatg agaatcgttc gtgaagaaag tgaattagaa
     1861 gatgctttcc atagagcaaa atcagaagct gaaaaatcat ttggtaatag tgaagtttac
     1921 atagaaagat acattgataa tccaaagcat attgaagtac aagtcatagg tgacgaacat
     1981 ggaaatatcg tacacttatt tgaacgtgat tgttcagtac aacgtcgtca tcaaaaagtt
     2041 gtagaagttg caccatcagt tggattatca tcaacattac gtcaacgtat ttgtgatgct
     2101 gcaattcaat tgatggaaaa tattaaatat gtcaatgcag gtactgttga atttctagta
     2161 tctggtgacg aattcttctt tatagaagtt aaccctcgtg tacaagtaga gcatacaatt
     2221 acagagatgg taacaggaat tgatattgtt aagacacaaa ttttagttgc agcaggtgcc
     2281 gatttatttg gtgaagagat taatatgccg caacaaaaag atattacaac attaggctat
     2341 gccatccaat gtcgtattac aacagaagat ccgttaaatg atttcatgcc ggatactgga
     2401 acaatcattg cttatcgttc aagcgggggc tttggtgtac gtcttgatgc tggagatggt
     2461 ttccaaggtg ctgagatatc accttattat gattcattac tcgtaaaatt atctacacac
     2521 gcgatatcat ttaaacaagc agaagaaaaa atggtacgct cattacgaga aatgcgtatt
     2581 cgtggtgtta aaactaatat tccattctta attaatgtaa tgaagaataa aaagttcaca
     2641 agtggtgatt acacaactaa atttattgaa gaaacaccag aacttttcaa cattcagccg
     2701 tctctagata gaggtactaa aacattagaa tatataggta atgtaacaat taatggtttc
     2761 ccaaatgttg agaaacgtcc gaaaccagac tatgaattag catcaattcc aactgtatct
     2821 tcaagtaaaa tcgcttcatt tagtggtacg aaacaattgc ttgatgaagt aggtccaaaa
     2881 ggtgtagctg aatgggttaa aaagcaggat gatgtcttac taacagatac aacctttaga
     2941 gatgcacacc aatcattatt agctacacga gttagaacta aggatatgat taatatcgca
     3001 tccaaaacag cggacgtatt taaagatggt ttctcactag aaatgtgggg cggtgctaca
     3061 tttgatgtgg catataattt cttgaaggaa aacccatggg aacgacttga acgtctacgt
     3121 aaagctattc caaatgtatt attccaaatg ttgttacgtg cttcaaacgc agttggttat
     3181 aaaaactatc ctgataatgt tattcataaa ttcgtacaag aaagtgctaa agcaggcata
     3241 gatgtcttta gaattttcga ttcattaaac tgggtagatc aaatgaaagt tgccaatgaa
     3301 gcagtacaag aagcgggcaa aatctcagaa ggtactattt gttatacagg tgacatttta
     3361 aatcctgagc gatcaaacat ttatacttta gagtattatg tcaaactagc taaagagtta
     3421 gaacgtgaag gtttccatat tttagcgatt aaagatatgg caggcttatt aaaacctaaa
     3481 gccgcttacg aattgattgg tgagttaaaa tcagctgtag atttaccaat tcatcttcac
     3541 actcatgata caagtggtaa tggtttatta acatacaaac aagcaataga tgctggtgtc
     3601 gatatcattg atactgctgt tgcttcaatg agtggtttaa caagtcagcc aagcgccaat
     3661 tcgttatatt atgcattaaa tggcttccca cgccacctta gaactgatat tgaaggtatg
     3721 gagtcactta gtcattattg gtcaactgta cgtacttatt attcagactt tgaaagtgat
     3781 atcaaatcac cgaatactga aatttatcaa catgaaatgc ctggtggaca gtattcgaat
     3841 ttaagtcaac aagctaaaag tttaggttta ggcgaaagat ttgatgaagt caaagatatg
     3901 tatcgcagag tgaatttctt atttggtgat atcgtaaaag taacaccatc gtctaaagta
     3961 gttggtgata tggcacttta tatggtacaa aatgatcttg atgaacaatc cgtgattaca
     4021 gatggctata aattagattt cccagaatca gtagtgtcgt tcttcaaagg tgaaatagga
     4081 caacctgtaa atggttttaa taaagattta caagcggtta ttttaaaagg ccaagaagca
     4141 ctaacagctc gtccaggtga atatctagag ccagttgatt ttgaaaaagt ccgtgagttg
     4201 cttgaagaag agcaacaagg tcctgttacg gagcaagata ttattagtta tgtactatat
     4261 ccaaaagtat atgaacaata tattcaaact agaaatcaat acggaaactt atcgttactt
     4321 gatacgccta cattcttctt tggaatgcgt aatggtgaaa cagtagaaat cgaaatcgat
     4381 aaaggtaaac gattaattat taaactagaa acgattagtg aaccagatga aaatggtaat
     4441 agaacgattt actatgcgat gaatggtcaa gcgagacgta tttacattaa agatgaaaat
     4501 gtgcatacaa atgcgaacgt taagccaaaa gcagataaga gtaatccaag tcatatcggt
     4561 gctcaaatgc caggttcagt aactgaagtc aaggttagtg taggtgaaac tgtgaaagct
     4621 aatcagccgt tgctaattac tgaagctatg aaaatggaaa caacaattca agcaccattt
     4681 gacggtgtga ttaaacaagt aactgtaaat aatggtgaca caatagcgac aggcgattta
     4741 ttaatcgaaa ttgaaaaagc aactgactaa aatgattaaa ataaaacgag attacacaac
     4801 aattgacgtg taatctcgtt tttacatggc ttaggtttaa ttttaaatat tgatataaaa
     4861 aaccgtaatt ttaatgggcg tgccattaaa attacggttt gcttttttgt tattgcttgt
     4921 cacttcttac tgatcgtaac attagcatga taaagtatgt tgtcatacca aataaataag
     4981 tgataaataa tgcatggaaa agtgcgatta tcaggttaac atttgtcata atagataatg
     5041 cacctgtgat aacttgtaat ataacaagta taaatgcagc tgtataacca taatgaacag
     5101 tacggttatt tggataattt ttaacagcgt gaatataagt aatcataata atcgtaaaca
     5161 ctataaaagc catgatacga tgcgtgagtt gaacccaatc ttgttctgaa tgtggtacaa
     5221 gatcgtggaa tggcaatggc caaccaccat atgctaaact tgcatccgca tgtctcacta
     5281 gtgcaccagt ataaacacca caataaatga tgattgccat taaccatgtt aaacgtctta
     5341 atggcttttt gatatataat tcgtcagctt catatttttg atctatagag aaaataatca
     5401 atgttattaa aaatacagat gagaaactga ttaatgatat accaaagtgc aatgctaaaa
     5461 cgtaatcgtt ttgttgccaa ataacagcag cagctccgat taatgcttgc aataataaga
     5521 atccaacact aatgattgat aaaggtttaa tttctttaat atagcctata tgtttccatg
     5581 cagtgataac taaccataag accattaata aagacaaagc tgaaacggct ctatgactta
     5641 actcaataat cgtatcaata ggaaagaatt ctggaatcaa cgcaccatga catagtggcc
     5701 aagaagaacc acaaccatca gctgatccgg ttttggtaac taaggctcca ccaagttgta
     5761 caaatgtcat cattaacgtt gctacgacac ctaaccattt taaattcttt ttgccaaaca
     5821 atttatacac cccatcataa aaaaacgcat cactgagaat tagaattaat agccggattt
     5881 gagatgatac tataaataga cctgattgta ggatatgaaa agatgggatc caacgcagga
     5941 aatatgattt aattttacaa gtattgtatg tggacgacat aaaaagaact taatcgtaat
     6001 gattttttta ttgggataat tatgtaatcc acgtttactc aaatttgaag cttaaatatt
     6061 aatctctttt agatgcatga ttctacttat taaaattttc taacatatac attatagcaa
     6121 taaattaaat gttatttgtg tcacaaaatt gacattttct cttgtcgtta atttgtcaca
     6181 atttaatttt attaaaccta g
//
LOCUS       DAKTIO010000155         6162 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.157, whole genome shotgun sequence.
ACCESSION   DAKTIO010000155 DAKTIO010000000
VERSION     DAKTIO010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.157"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(2..109)
                     /locus_tag="O0293_002248"
     CDS             complement(2..109)
                     /locus_tag="O0293_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281119.1"
                     /translation="MVLLAFVTTIHDTDDGRFFNEHKHANNKLLKHSLI"
     gene            complement(146..469)
                     /gene="isdG"
                     /locus_tag="O0293_002249"
     CDS             complement(146..469)
                     /gene="isdG"
                     /locus_tag="O0293_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdG"
                     /protein_id="HCY0281120.1"
                     /translation="MKFMAENRLTLTKGTAKDIIERFYTRHGIETLEGFDGMFVTQTL
                     EQEDFDEVKILTVWKSKQAFTDWLKSDVFKAAHKHVRSKNEDESSPIINNKVITYDIG
                     YSYMK"
     gene            complement(488..1222)
                     /gene="srtB"
                     /locus_tag="O0293_002250"
     CDS             complement(488..1222)
                     /gene="srtB"
                     /locus_tag="O0293_002250"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HCY0281121.1"
                     /translation="MRMKRFLTIVQILLVVIIIIFGYKIVQTYIEDKQERANYEKLQQ
                     KFQMLMSKHQEHVRPQFESLEKINKDIVGWIKLSGTSLNYPVLQGKTNHDYLNLDFER
                     EHRRKGSIFMDFRNELKNLNHNTILYGHHVGDNTMFDVLEDYLKQSFYEKHKIIEFDN
                     KYGKYQLQVFSAYKTTTKDNYIRTDFENDQDYQQFLDETKRKSVINSDVNVTVKDRIM
                     TLSTCEDAYSETTKRIVVVAKIIKVS"
     gene            complement(1284..2249)
                     /gene="isdF"
                     /locus_tag="O0293_002251"
     CDS             complement(1284..2249)
                     /gene="isdF"
                     /locus_tag="O0293_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin ABC transporter permease protein IsdF"
                     /protein_id="HCY0281122.1"
                     /translation="MIKNKKKLLFLCLLVILIATAYISFVTGTIKLSFNDLFTKFTTG
                     SNEAVDSIIDLRLPRILIALMVGAMLAVSGALLQAALQNPLAEANIIGVSSGALIMRA
                     LCMLFIPQLYFYLPLLSFIGGLIPFLIIILLHSKFRFNAVSMILVGVALFVLLNGVLE
                     ILTQNPLMKIPQGLTMKIWSDVYILAVSALLGLILTLLLSPKLNLLNLDDIQARSIGF
                     NIDRYRWLTGLLAVFLASATVAIVGQLAFLGIIVPHVVRKLVGGNYRVLIPFSTVIGA
                     WLLLVADLLGRVIQPPLEIPANAILMIVGGPMLIYLICQSQRNRI"
     gene            complement(2262..3140)
                     /gene="isdE"
                     /locus_tag="O0293_002252"
     CDS             complement(2262..3140)
                     /gene="isdE"
                     /locus_tag="O0293_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HCY0281123.1"
                     /translation="MRIIKYLTILVISVVILTSCQSSSSQESTKSGEFRIVPTTVALT
                     MTLDKLDLPIVGKPTSYKTLPNRYKDVPEIGQPMEPNVEAVKKLKPTHVLSVSTIKDE
                     MQPFYKQLNMKGYFYDFDSLKGMQKSITQLGDQFNRKAQAKELNDHLNSVKQKIENKA
                     AKQKKHPKVLILMGVPGSYLVATDKSYIGDLVKIAGGENVIKVKDRQYISSNTENLLN
                     INPDIILRLPHGMPEEVKKMFQKEFKQNDIWKHFKAVKNNHVYDLEEVPFGITANVDA
                     DKAMTQLYDLFYKDKK"
     gene            complement(3137..4213)
                     /gene="isdD"
                     /locus_tag="O0293_002253"
     CDS             complement(3137..4213)
                     /gene="isdD"
                     /locus_tag="O0293_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated surface determinant protein IsdD"
                     /protein_id="HCY0281124.1"
                     /translation="MRNVKQIATKSIIAIISLGILTYTTMIGSVLADEIKYPSAKFNQ
                     PEAKDKTELTTSIFDEKIKENKALELLIFNQENKNVTEEQQLVDEKAQLISDMTGKIY
                     LQVKLKGQIDKEQLVFQNDKNEEFPFVIKDEKDDTIVRILIEQHMDKINMHVKTLAEK
                     KNLDNKEMVYSIHFKEKKVQHDDAKEVPSKHQNQENNQDQLKKDIDDKKDSQKSDTKE
                     RRTSLFTEKGLNDIPVQKDKVQQDSNKKIENERPKASGTLKVENSPPTIKKVENNHKE
                     QPKHKDEKSKKEKKKVVEKEKALPAFNRDDDSKNSSQLSSDIKELDEPNHKKQYMLFA
                     AGIVLATILLISAHLYSRKRGNQV"
     gene            complement(4213..4896)
                     /gene="isdC"
                     /locus_tag="O0293_002254"
     CDS             complement(4213..4896)
                     /gene="isdC"
                     /locus_tag="O0293_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdC"
                     /protein_id="HCY0281125.1"
                     /translation="MKNILKVFNTTILALIIIIATFSNSANAADSGTLNYEVYKYNTN
                     DTSIANDYFNKPAKYIKKNGKLYVQITVNHSHWITGMSIEGHKENIISKNTAKDERTS
                     EFEVSKLNGKIDGKIDVYIDEKVNGKPFKYDHHYNITYKFNGPTDVAGANAPGKDDKN
                     SASGSDKGSDGTTTGQSESNSSNKDKVENPQTNAGTPAYIYAIPVASLALLIAITLFV
                     RKKSKGNVE"
     gene            5105..6157
                     /gene="isdA"
                     /locus_tag="O0293_002255"
     CDS             5105..6157
                     /gene="isdA"
                     /locus_tag="O0293_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored heme-scavenging protein IsdA"
                     /protein_id="HCY0281126.1"
                     /translation="MTKHYLNSKYQSEQRSSAMKKITMGTASIILGSLVYIGADSQQV
                     NAATEATNATNNQSTQVSQATSQPINFQVQKDGSSEKSHMDDYMQHPGKVIKQNNKYY
                     FQTVLNNASFWKEYKFYNANNQELATTVVNDNKKADTRTINVAVEPGYKSLTTKVHIV
                     VPQINYNHRYTTHLEFEKAIPTLADAAKPNNVKPVQPKPAQPKTPTEQTKPVQPKVEK
                     VKPTVTTTSKVEDNHSTKVVSTDTTKDQTKTQTAHTVKTAQTAQEQNKVQTPVKDVAT
                     AKSESNNQAVSDNKSQQTNKVTKHNETPKQASKAKELPKTGLTSVDNFISTVAFATLA
                     LLGSLSLLLFKRKESK"
BASE COUNT         2002 a         1095 c          795 g         2270 t
ORIGIN      
        1 ttcaaatcaa actatgtttt agcaatttgt tgttagcatg tttgtgttca ttaaaaaaac
       61 gaccatcatc ggtatcatgt atggtcgtta caaaagctaa caataccaat tgtcataaca
      121 agtactgcaa cctctttaaa ttcaattatt tcatgtaact atagcctata tcatatgtaa
      181 ttactttgtt atttataatc gggctacttt catcttcatt tttacttcta acatgtttat
      241 gcgctgcttt aaagacatca gattttaacc aatccgtaaa agcttgcttt gatttccaaa
      301 ctgttaaaat tttcacttca tcaaaatctt cttgttctaa agtttgtgta acaaacatgc
      361 catcaaagcc ttctaatgtt tcaatcccat gtctcgtgta aaatcgttct ataatatctt
      421 ttgctgttcc ttttgttaac gtcagcctat tttctgccat aaatttcata attatcctct
      481 tttctgttta acttacctta attatttttg cgacaacaac aattcttttc gtcgtttcac
      541 tatatgcatc ttcgcacgtt gataaagtca ttattctatc ttttaccgtt acattaacat
      601 ctgaattaat tacagattta cgttttgtct catctaaaaa ttgttgataa tcttgatcat
      661 tttcaaaatc tgtacgtatg taattatctt tagtagtagt tttatatgca ctaaatactt
      721 gcaattgata tttaccatat ttattgtcaa attcaattat cttgtgtttt tcataaaacg
      781 attgctttaa ataatcttct aacacatcaa acatcgtatt atcaccgaca tggtgcccgt
      841 ataaaatagt attatgattt aaattcttca attcatttct aaaatccata aaaatactac
      901 ctttacgtcg atgttctcgc tcaaaatcta aatttaaata atcgtgattt gtcttacctt
      961 gtagtactgg ataatttaat gatgttcctg ataattttat ccatccaaca atgtctttat
     1021 ttattttttc aagtgattca aattgtggtc tcacatgttc ttgatgtttg ctcatcagca
     1081 tttgaaattt ttgttgtaat ttctcataat ttgcgcgttc ttgcttgtct tcaatatatg
     1141 tttgaacaat tttgtaacca aaaatgataa taattacaac caataaaatt tgtacaatag
     1201 ttaaaaatcg cttcattctc ataaaaatcc tcttttatta acgacgtttc ttcagtcatc
     1261 actaaaccag ttgttgtacc gttttagatt cgatttcgtt gactttgaca aattaagtaa
     1321 attagcattg gaccaccgac aatcattaaa atagcattgg ctggaatttc taaaggaggc
     1381 tgtatcactc gtcctaataa atcagccact aacaatagcc atgcaccaat aactgtagaa
     1441 aacggaataa gtactctgta attgccccca actagctttc taaccacatg tggcacaata
     1501 atacctaaaa aggctagttg tccaacaatc gcaacagttg cacttgctaa aaatactgct
     1561 aataaacctg ttaaccatct gtaacgatca atattaaaac cgatacttcg cgcttgtatg
     1621 tcgtctaaat ttagtaaatt caatttaggg gacaatagta atgttaatat taatcccaat
     1681 aatgctgata ctgctaatat gtatacgtcg ctccatattt tcattgttaa gccttgagga
     1741 attttcatta aagggttttg agttaaaatt tctaaaacac catttaataa tacgaataac
     1801 gcaacaccta ctaatatcat acttacagca ttgaatctaa atttagaatg caacaatata
     1861 attattaaaa atggtattaa acctccaata aaacttaata atggtaagta aaagtacaat
     1921 tgtggaataa acaacataca aagtgctctc attataagtg cacctgagga aacgccaatg
     1981 atattcgcct ctgccaaagg attttgtagt gctgcttgta ataatgctcc agaaactgct
     2041 aacattgcgc caaccatcaa tgcaattaat atacgtggca atcgcaaatc aatgattgaa
     2101 tccactgctt cattgctacc agttgtaaat tttgtaaata ggtcattaaa tgacaattta
     2161 attgtaccgg ttacaaacga aatataagca gttgcgatta aaatgactaa caaacataaa
     2221 aatagtagtt tctttttatt ttttatcatc atatcaactc actatttttt atccttataa
     2281 aataaatcat ataattgagt cattgcctta tcagcatcaa catttgctgt aataccgaat
     2341 ggcacttcct ctaagtcata aacatgatta tttttcacag ctttaaaatg tttccaaata
     2401 tcattttgtt taaattcttt ttgaaacatt ttcttaactt cttcaggcat tccgtgtggt
     2461 aatcgtaaaa taatatctgg attgatattc aacaaatttt cagtattaga cgaaatatat
     2521 tgacgatctt tcactttaat aacattttct ccacctgcta ttttaactaa atcaccaata
     2581 tatgatttat cagttgctac taaatagcta cccggtacac ccattaatat taatactttg
     2641 ggatgtttct tttgtttagc tgctttattt tcaatttttt gctttacaga atttaaatgg
     2701 tcatttaatt cttttgcttg tgctttacga ttaaattgat cacctaattg tgtaatcgac
     2761 ttttgcatcc cttttaaact atcaaaatca taaaagtagc ctttcatatt taattgtttg
     2821 taaaatggtt gcatttcatc tttaatcgtt gacacactca aaacatgtgt tggttttaac
     2881 tttttaacag cttcaacatt cggctccatt ggttgaccaa tttccggtac atctttataa
     2941 cgattaggca atgtcttata tgacgtgggt ttgccgacaa ttggtaaatc caatttgtcc
     3001 aatgtcattg tcaatgcaac agttgttggt acgattctga attcgccgga tttagttgat
     3061 tcttgagaac tggaagattg acagctggtt aagataacga cgcttatcac taaaatggtt
     3121 aaatacttta tgattctcaa acttggttac ctctctttct gctgtataaa tgtgccgaaa
     3181 taagtaaaat agttgctaac acaatgccag ctgcaaataa catatattgc tttttatgat
     3241 ttggttcatc aagttcttta atatcactag ataattgact actattcttg ctatcatcat
     3301 ctctattaaa agctggtaac gctttttctt tttcaactac ttttttcttt tccttttttg
     3361 atttttcatc tttatgtttc ggttgctctt tgtgattatt ttcaaccttt tttattgttg
     3421 gagggctatt ttcaactttt aatgtacctg atgctttagg tcgctcattt tcaatctttt
     3481 tattactgtc ttgttgcact ttatcttttt gtacaggaat atcatttaat cctttttcag
     3541 taaaaaggct agtacgtctt tccttagtat ctgatttttg actatctttt ttgtcatcaa
     3601 tatctttttt aagctgatct tgattatttt cttgattttg atgttttgaa ggcacttctt
     3661 ttgcatcatc atgttgtact tttttctctt taaaatgaat agaatacacc atttctttgt
     3721 tatctagatt tttcttttca gccaacgttt taacatgcat attgatttta tccatatgct
     3781 gttcaattaa aattcttact attgtgtcat ccttttcatc ttttataaca aaaggaaatt
     3841 cttcattttt gtcattttga aaaacaagtt gttctttatc tatttgacct tttagcttta
     3901 cttgcaagta aattttacca gtcatatctg aaatcaattg cgccttttcg tcaactagtt
     3961 gttgttcctc agttacattt ttattttctt gattaaaaat tagtaactct aacgctttat
     4021 tctcttttat cttttcatca aaaattgatg tagttaattc tgttttatct tttgcttcag
     4081 gttgattaaa tttggctgat ggatatttta tctcatcagc caacacgcta ccaatcattg
     4141 ttgtatatgt aagtatacct aagctaataa tagctataat agattttgta gcaatttgtt
     4201 taacatttct cattattcca cattgccttt agattttttt ctaacaaaca atgtgattgc
     4261 gattaataat gctaaggatg caactggtat tgcatatata tatgcaggtg taccagcatt
     4321 tgtttgtgga ttttctactt tgtctttatt cgaactatta gattcacttt gaccagtagt
     4381 cgttccatca gatcctttgt cactacctga agcagaattt ttatcatctt tacctggtgc
     4441 attagcacct gctacatcag ttggtccatt aaatttatat gtaatgttgt aatgatggtc
     4501 atatttgaat ggctttccat ttactttttc atcgatataa acgtcaattt ttccatctat
     4561 tttaccgttc aacttactta cttcaaattc agaagtgcgt tcatctttgg cagtgttttt
     4621 actaataata ttttctttat gtccttcgat actcattcca gtaatccaat gactgtggtt
     4681 gacagttatt tgaacataca atttaccatt tttcttaatg tactttgccg gtttattaaa
     4741 atagtcatta gcaattgacg tgtcattggt attgtatttg taaacctcat aattcaaagt
     4801 accgctatct gcggcatttg cagaattact gaatgtcgcg atgatgataa ttaacgctaa
     4861 aatcgttgta ttaaaaactt ttaaaatatt tttcaaaaca taatcctcct ttttatgatt
     4921 gcttttaagt ctttagtaaa atcataaata ataatgatta tcattgtcaa tatttatttt
     4981 ataatcaatt tattattgtt atacgaaaat agatgtgcta gtataattga taaccattat
     5041 caattgcaat ggttaatcat ctcatataac aacacataat ttgtatcctt aggaggaaaa
     5101 caacatgaca aaacattatt taaacagtaa gtatcaatca gaacaacgtt catcagctat
     5161 gaaaaagatt acaatgggta cagcatctat cattttaggt tcccttgtat acataggcgc
     5221 agacagccaa caagtcaatg cggcaacaga agctacgaac gcaactaata atcaaagcac
     5281 acaagtttct caagcaacat cacaaccaat taatttccaa gtgcaaaaag atggctcttc
     5341 agagaagtca cacatggatg actatatgca acaccctggt aaagtaatta aacaaaataa
     5401 taaatattat ttccaaaccg tgttaaacaa tgcatcattc tggaaagaat acaaatttta
     5461 caatgcaaac aatcaagaat tagcaacaac tgttgttaac gataataaaa aagcggatac
     5521 tagaacaatc aatgttgcag ttgaacctgg atataagagc ttaactacta aagtacatat
     5581 tgtcgtgcca caaattaatt acaatcatag atatactacg catttggaat ttgaaaaagc
     5641 aattcctaca ttagctgacg cagcaaaacc aaacaatgtt aaaccggttc aaccaaaacc
     5701 agctcaacct aaaacaccta ctgagcaaac taaaccagtt caacctaaag ttgaaaaagt
     5761 taaacctact gtaactacaa caagcaaagt tgaagacaat cactctacta aagttgtaag
     5821 tactgacaca acaaaagatc aaactaaaac acaaactgct catacagtta aaacagcaca
     5881 aactgctcaa gaacaaaata aagttcaaac acctgttaaa gatgttgcaa cagcgaaatc
     5941 tgaaagcaac aatcaagctg taagtgataa taaatcacaa caaactaaca aagttacaaa
     6001 acataacgaa acgcctaaac aagcatctaa agctaaagaa ttaccaaaaa ctggtttaac
     6061 ttcagttgat aactttatta gcacagttgc cttcgcaaca cttgcccttt taggttcatt
     6121 atctttatta cttttcaaaa gaaaagaatc taaataaatc at
//
LOCUS       DAKTIO010000156         6044 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.158, whole genome shotgun sequence.
ACCESSION   DAKTIO010000156 DAKTIO010000000
VERSION     DAKTIO010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6044
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.158"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            132..1586
                     /locus_tag="O0293_002256"
     CDS             132..1586
                     /locus_tag="O0293_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0281127.1"
                     /translation="MEDNKMNRSLNSRHISMIAIGGAIGTGLFVATGNIISQAGPGGA
                     ILAYLVIGVMLYFLMSSIGELATFYPVSGSFSSYSTRFIDSSLGFTMGWLYWALWSLV
                     TSVDVIVASNVLYFWDTFKFFHPITWSLIFITILLLLNIFSVKSFGETEFWLSLIKVL
                     TIIVFVIFGFLMIFGILGGHTYGFENYTKGQAPFVGGISGFLGVLLVAGFSVGGTEVV
                     AVTAGESDDPKKSMPKAIKQVFWRILLFYVLSIAVIGAIIPYTDPSLLRASSSISQSP
                     FTIVFDRVGIAFAASVINAVILTSLLSAANSGVYTTGRMLYSLSSDKKAPQFLSKLNK
                     TTKLPLRALLTTYAVVVIVIIYANFNSNAVFNLLEIIGSMIIVVWGSSIWSQIRLRQA
                     IKKQGQDPNKVLPYKAPFYPLGPIIVITTLLFLLFGGSVEYILKDQWLNAFKNFLPLI
                     ILALIYFIHKIIHKTKFVKLETINLKPHDYDNQK"
     gene            1725..1829
                     /locus_tag="O0293_002257"
     CDS             1725..1829
                     /locus_tag="O0293_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281128.1"
                     /translation="MLPFFILDILHIVYNIDNNNFSGVKFNDWTNKFI"
     gene            1804..2118
                     /locus_tag="O0293_002258"
     CDS             1804..2118
                     /locus_tag="O0293_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281129.1"
                     /translation="MIGQTNLFDDVLKQDERFVIVVQALEEKNGQLLKRTLREYPGLN
                     HKQMNDLFMHLKELFSEESFAENQSAFSITVYTNLDYTADQIYAHVKRFRGKHDWTQT
                     AK"
     gene            complement(2411..3214)
                     /locus_tag="O0293_002259"
     CDS             complement(2411..3214)
                     /locus_tag="O0293_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCY0281130.1"
                     /translation="MTNYKVVVLDMDDTLLNSDNVISEETANYLTAIQDEGYYVVLAS
                     GRPTEGMIPTARDLKLPEHHSYIISYNGSKTINMTNEEVEVSKSIGKQDFDEIVDYCR
                     DRGFFVLTYHDGQIIYDSEHEYMNIEAELTGLPMKRVDDIKAYIQGDVPKVMGVDYVA
                     NITEARIDLNGVFNDNVDATTSKPFFLEFMAKDVSKGNAIKVLCHKLGYSVDQVIAFG
                     DSMNDKSMFEVAGLAIAMGNASDELKQYANEVTLDHNENGIPHALKKLL"
     gene            complement(3272..4186)
                     /locus_tag="O0293_002260"
     CDS             complement(3272..4186)
                     /locus_tag="O0293_002260"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCY0281131.1"
                     /translation="MSNVLELTIPASTANLGVGFDSIGMALDKFLHLSVKETSGTKWE
                     YIFHDDASKQLPTDETNFIYHVAQQVASKYSVDLPNLCIEMRSDIPLARGLGSSASAL
                     VGAIYIANYFGDIQLSKHEVLQLATEIEGHPDNVAPTIYGGLIAGYYNDVSKETSVAH
                     IDIPDVDVIVTIPTYELKTEASRRALPQKLTHSEAVKSSAISNTMICALAQHNYELAG
                     KLMQQDGFHEPYRQHLIAEFDEVKTIASQHNAYATVISGAGPTILIFSRKENSGELVR
                     SLNSQVVSCHSELVDINISGVKERIVYQ"
     gene            complement(4188..5249)
                     /locus_tag="O0293_002261"
     CDS             complement(4188..5249)
                     /locus_tag="O0293_002261"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCY0281132.1"
                     /translation="MRRWQGLVEEFKAHLPVNENTPKLTLNEGNTPLIHCENMSKILG
                     IDLYVKYEGANPTGSFKDRGMVMAVTKAKEQGKKIVICASTGNTSASAAAYAARAGLK
                     AIVVIPEGKIALGKLSQAVMYGAEIVSIEGNFDEALEIVKEIAKSGEIELVNSVNPFR
                     IEGQKTGSFEIVQQLDGEAPDILAIPVGNAGNITAYWKGFKEYHEAKGSQLPKMFGFQ
                     AEGASPIVQNKVIKNPETIATAIRIGNPASWDKATNALKESNGLIDSVTDDEILEAYQ
                     LMTTKEGVFSEPASNASIAGLIKLHRQGKLPQGKKVVAILTGNGLKDPDTAISLLDNP
                     IKPLPNDKDSIIDYIKGAL"
     gene            complement(5255..>6044)
                     /locus_tag="O0293_002262"
     CDS             complement(5255..>6044)
                     /locus_tag="O0293_002262"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HCY0281133.1"
                     /translation="ILSKMTKEQTTFEEALDEAKRLGFAEADPTDDVEGVDAARKVVI
                     TSYLSFNQVIKLNDVKRRGISGVTLTDINVADQLGYKIKLIGKGIYENGKVNASVEPT
                     LIDKKHQLAAVEDEYNAIYVIGDAVGDTMFYGKGAGSLATGSAVVSDLLNVALFFESD
                     LHTLPPHFELKTDKTREMMDSDAEINIKEKSNFFVVVNHVKGSIENFENELKAILPFH
                     RSLRVANYDNQSYAAVIVGLESSPEELITKHGYEVDKVYPVEGV"
BASE COUNT         1873 a         1117 c          882 g         2172 t
ORIGIN      
        1 taaacatgct caagatgatt aaaaaatcag aaaattttaa ttttttcgct ttacagtact
       61 aaatagttat tgaatagtac acttaaaaag cttacaataa tgggtatatt taaatagatg
      121 ggagactaaa tatggaagat aataaaatga accgtagtct taactcaaga cacatttcca
      181 tgattgctat aggtggtgca attgggactg gtctatttgt agctactggt aatatcattt
      241 ctcaagctgg tcctggaggc gctatactcg cttatcttgt tattggtgtc atgctatatt
      301 tcttaatgtc atcaattgga gagttggcaa cattttatcc agtatcaggt tcattcagct
      361 cttattcaac acgctttatt gactcatctc ttggctttac catgggatgg ttgtattggg
      421 cattgtggtc attagttaca agtgttgatg tcatagtagc gtcaaatgtg ctttatttct
      481 gggacacatt taaatttttc caccccatta cttggagctt aatctttatt acaattttac
      541 tattattaaa cattttttct gtaaaatcat ttggagaaac tgagttttgg ttatcattga
      601 ttaaagtgtt aacaattatc gtattcgtta tttttggctt tttaatgatt ttcggtatct
      661 taggtggtca tacatatgga tttgaaaact atacaaaagg ccaagcaccg tttgttggtg
      721 gtatctctgg tttcttaggc gtattattag tcgccggatt ttcggttggt ggtacagaag
      781 tagtagcagt aactgctggt gaatcagatg accctaaaaa gtctatgcct aaggcaatta
      841 aacaagtatt ttggcgtatt cttttattct atgtcttatc aattgcagta attggtgcaa
      901 ttattccgta cacagatcca tcattattaa gagcaagtag ttcaataagt caaagcccat
      961 ttacaattgt attcgataga gtaggcatag cctttgcagc atcagtaatc aacgcggtta
     1021 ttttaacttc attattatcc gctgcaaatt caggtgttta tacaacaggc agaatgttgt
     1081 attccttaag ttcagacaaa aaagcacccc aatttttaag taaattaaac aagacaacta
     1141 agttaccttt aagagcatta ttaactactt atgcagtcgt tgttattgtt attatttatg
     1201 caaactttaa ttcaaatgcc gtttttaatt tacttgaaat tattggttca atgattatag
     1261 ttgtttgggg atcaagcatt tggtcacaaa tacgattgcg acaagctatt aaaaaacaag
     1321 gtcaagaccc taataaggtc ctaccatata aagcaccttt ttatccatta ggaccaatca
     1381 ttgtcatcac tacactatta ttcttgctat ttggtggctc agttgaatat attttaaaag
     1441 atcaatggtt aaatgctttt aaaaactttt tacctttaat cattctagcg ttgatttact
     1501 ttattcataa aatcattcac aaaacaaaat ttgtaaagct agaaacaatt aatttaaaac
     1561 cacacgatta tgacaatcaa aaataaataa attctcatac ggcagatgct ttagattatc
     1621 tatagcattt gccgcttttt aatggtgtaa agcagatata ctttaatatc tttttctagt
     1681 ataaagtgta ttaactagta ttctgtttat attttattat taccatgtta ccattcttta
     1741 ttttagatat tttgcatatc gtgtataata tagataacaa taacttttca ggggtgaaat
     1801 tcaatgattg gacaaacaaa tttatttgat gatgtactaa agcaagatga aagatttgtt
     1861 attgtagttc aagcattaga agaaaagaac ggacaattat taaagagaac tttaagggaa
     1921 tatcccggtt taaaccataa acaaatgaat gatttattta tgcacttaaa ggaattattt
     1981 tccgaagaat catttgctga aaaccaatca gcgttcagta ttacagttta tacaaattta
     2041 gattatactg ctgaccaaat atatgctcat gtaaaacgtt tcagaggtaa gcatgactgg
     2101 acacaaacag ctaaataaaa tcaagatgct tgcagtgatt ttaacaatta atgttagatt
     2161 gtcactgcat tttttaatgc attcgttatt attcagaatg atcaaattta tttatctacc
     2221 ttccgtgact agttaatact tgatttattc aaaaaatata catttactct attcgtttga
     2281 tatcattact aatctaaaat atgtatttca aatcctccat agtataactg aagtcatttt
     2341 cacaaaaata aaaagcctta accacaagat atcttattca aatatcatgt gttaaggctt
     2401 attttaaaat ttataacaat tttttgagcg catgtggaat accattttca ttatgatcca
     2461 acgtaacttc atttgcatat tgcttaagtt catctgatgc attccccata gcaatagcta
     2521 gacctgcgac ttcaaacatt gatttatcat tcatactatc accaaaagca atgacttgat
     2581 ccaccgaata tcccaatttg tgacataaca ctttaattgc attacctttt gaaacgtctt
     2641 tggccataaa ttctaagaag aatggcttac ttgtcgtagc atctacatta tcattgaaca
     2701 caccattcaa atcaattcta gcttctgtaa tattcgctac ataatctaca cccatgacct
     2761 tgggtacatc gccttgaata tacgctttga tatcatcaac acgtttcatc ggtaaacctg
     2821 ttaattctgc ttcaatattc atatactcat gttcgctgtc gtaaataatt tgaccatcat
     2881 gatatgtaag aacgaaaaag cctctatctc gacaataatc tacaatttca tcgaaatctt
     2941 gcttaccaat cgatttactt acttctactt cttcattagt catgttaatc gttttactac
     3001 cgttataact aataatatag ctatgatgtt caggtaattt taaatctcta gcagttggaa
     3061 tcataccttc agtaggtcta ccagatgcta gaacaacata ataaccttca tcttgaattg
     3121 ctgttaaata atttgcagtt tcttctgata tcacattatc tgaatttagc aatgtgtcat
     3181 ccatatctaa aacgacaact ttataatttg tcatatcgca ttaaatctcc tctctattct
     3241 gcatttaata ctattttaca atataaagcg tctattggta tacaattcgt tctttaacac
     3301 cactgatatt aatatcgacc aattcagaat ggcatgatac tacctgacta tttaaagagc
     3361 gaaccaattc cccactattt tctttacgac taaatattaa aatagttggt ccagcaccac
     3421 taattacagt tgcataggca ttatgttgac tagcaattgt tttcacttca tcaaattcag
     3481 caattaaatg ctgacgatac ggttcatgaa agccatcttg ttgcatgagt ttacctgcta
     3541 attcataatt gtgctgtgct aatgcacaaa tcattgtatt actaattgca ctacttttaa
     3601 ccgcttcact atgtgttaat ttttgtggta aagcacgtct tgatgcttct gtttttagtt
     3661 cataagttgg tatcgttaca atcacatcca cgtctggtat gtcgatatgt gcaactgacg
     3721 tttctttcga gacatcatta taatatccag cgattaaacc accataaatg gtcggcgcaa
     3781 cattatcagg atgtccttcg atttcagtcg ctaattgtaa tacctcatgt ttagacagtt
     3841 ggatatcacc aaaataattt gcgatatata tagctcctac taaagcagaa gctgacgaac
     3901 ctaaccctct tgccaatgga atatcacttc tcatttcgat acataaatta ggcaagtcaa
     3961 cactatattt agaagcaact tgttgtgcta catgataaat aaagtttgtt tcgtcagtag
     4021 gcaattgctt agatgcatca tcatggaaaa tatattccca ttttgtccct gatgtttcct
     4081 ttacagacag atgcaaaaat ttatctaaag ccatacctat agaatcaaag ccaactccaa
     4141 ggttggctgt tgatgcagga attgttaact ccaaaacatt cgacatgtta taaagctcct
     4201 ttaatataat cgataatgct atctttatca tttggcaatg gctttatcgg attatctagt
     4261 agtgaaatag cagtatcagg atcttttaat ccattaccag ttaaaatagc aactactttt
     4321 ttaccttgag gtaatttacc ttgtctatgc aatttaatta aacctgcaat agaagcatta
     4381 ctcgctggtt cactaaagac accttcttta gttgtcatta actgatatgc ttctagaatt
     4441 tcatcatcag taacactatc tattaatcca tttgattctt taagagcatt agtcgcctta
     4501 tcccaactag caggattacc aattcgaata gcagttgcaa tcgtttcagg atttttaatg
     4561 actttatttt gaacaattgg tgatgcgcct tcagcttgga agccaaacat tttcggcaat
     4621 tgtgatcctt tagcttcatg atattcttta aagcctttcc aatatgcagt aatattacct
     4681 gcattaccta caggaatcgc taaaatatca ggtgcttcac cgtctaattg ttgtacaatt
     4741 tcaaatgagc ctgtcttttg tccttcgatt ctaaatggat tgacagagtt tacaagctcg
     4801 atttcgccac tttttgcaat ttcttttaca atttctaaag cttcatcaaa gtttccttca
     4861 atagaaacga tttctgcacc atacattact gcttgcgaca atttacctaa tgcaatttta
     4921 ccttctggta ttacgacgat agcttttaaa cctgctctcg ctgcatatgc tgctgcagac
     4981 gctgatgtat ttccagtcga agcgcatatt acaattttct taccttgctc ttttgctttt
     5041 gtcacagcca ttaccatacc gcgatcttta aatgaacctg tcggattggc accttcatac
     5101 ttcacatata aatctatgcc tagtatttta gacatatttt cacaatgaat gagtggtgta
     5161 tttccctcgt tcaatgttaa ttttggtgta ttttcattta ctggtaaatg tgctttaaac
     5221 tcttctacta atccttgcca tcttctcatt ataattaaac tccttctact gggtatactt
     5281 tgtcaacttc gtatccatgc ttagtgatta attcttccgg tgatgattcc aatccaacta
     5341 taacagcggc atatgattga ttatcgtaat ttgcaactct taatgatcgg tgaaatggta
     5401 atattgcctt taactcattt tcaaaatttt caattgaacc tttgacatga ttcactacta
     5461 caaagaaatt ggatttttct ttaatattaa tttctgcatc tgaatccatc atttcccgtg
     5521 ttttatctgt ctttaattca aaatgtggtg gcaatgtgtg taaatctgat tcaaagaata
     5581 atgctacatt caataaatca ctgacaacgg cactacctgt tgctaaactg cctgctcctt
     5641 ttccataaaa catcgtgtca ccaacggcat caccaataac ataaatcgcg ttatattcat
     5701 cctctacagc tgctaattga tgctttttat caattaacgt tggttctacc gatgcattaa
     5761 ctttgccatt ttcatatatt cccttaccaa tcaatttaat tttatacccc agttgatcgg
     5821 ctacattaat atcagttaaa gttacgccac taattcctct tcgtttaacg tcgtttaatt
     5881 taatgacttg gttaaatgat aaatatgatg taatgacaac tttacgcgct gcatctaccc
     5941 cttctacatc atcagttgga tccgcttcag caaaaccaag tcttttcgct tcatcaagtg
     6001 cttcctcaaa tgtcgtttgc tctttagtca ttttagataa aata
//
LOCUS       DAKTIO010000157         6011 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.159, whole genome shotgun sequence.
ACCESSION   DAKTIO010000157 DAKTIO010000000
VERSION     DAKTIO010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.159"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(109..987)
                     /locus_tag="O0293_002263"
     CDS             complement(109..987)
                     /locus_tag="O0293_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HCY0281134.1"
                     /translation="MPNKILLVDGMALLFRHFYATSLHKQFMYNSQGEPTNGIQGFVR
                     HIFSAIHEIRPTHVAVCWDMGQSTFRNDMFDGYKQNRSAPPEELIPQFDYVKEISEQF
                     GFVNIGVKNYEADDVIGTLAQQYSTDNDVYIITGDKDLLQCINDNVEVWLIKKGFNIY
                     NRYTLHRFNEEYALEPQQLIDIKAFMGDTADGYAGVKGIGEKTAIKLIQQYQSVENVV
                     ENIDALSAGQRNKINDNLDELYLSKRLAEIHTQVPIDSEALFEKMSFATTLNHILSIC
                     NEHELHVSGKYISSHF"
     gene            complement(1007..4447)
                     /locus_tag="O0293_002264"
     CDS             complement(1007..4447)
                     /locus_tag="O0293_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HCY0281135.1"
                     /translation="MINKEQLDLLYKLKKEVEKSRNEALLHTINQVIKKVYLQQYTCS
                     FVGHFSAGKSTLINLLIEQDILPSSPVPTTSNTAIVSVSDNHDIIANLPNQTYAKLSN
                     YDEVREMNRQNVDVESVEINFQSAKFENGFTLQDTPGVDSNVASHQSITEQYMYTSNM
                     IFYTVDYNHVQSELNFKFMKHINDVGIPVVFIINQIDKHQDDELSFSTFKSRVEKSIA
                     DWGIKLERTFYVSKFDHPENELEVLSSYLISLDQHRETIEDYTSRTVEYITEAQLDYI
                     QSEIQEVLEDLGIEEAEFEQAFLNSQQHQAISEEAQLLNNPDELMAFLKNKRKNILEN
                     AYIMPHNMREMLRSYLESMSQDFNVGGFFNKKKKKLQIQQQRLLTATDALQEHVNQQI
                     RQPMREDMSFVTRFINKKEASDKVLNQHYDVKPEMIEGLYQPQTSISNTYVLTFSDEV
                     VKAIKKYVEQQSTPIFKEIIENVQADELPTEESDDLKEYQRYTELNELRQSLTTKNYR
                     HYYIHLDESLDKLIGRQETTYQVATDNAQDNRDNQQLNQNTATTNMSIDIQKALDIIS
                     DVPLFKRTKQDIHETLTRIDNKLIKIGVFGTFSAGKSSLINALLGEHILVSSPNPTTA
                     ATTEISYGDESYITLKSQSQLLDEINAVVEYQDMSYSTIEDFINSDLEKLKSHLNKNQ
                     LAFIQAVEKHYKLYVNMLENGEKHAINQQELKKWSAEDEYATFVKTVHIALMHDWLKG
                     KIIVDSLGLHSNNQRHTNETEQILTSSDLILYVSYFNHSFTDNDKAFIEHMKDMNQLN
                     ENQALKMVINAADLAESQDDLEAVETYVSDALRQVHLQSDIFAVSSRNALQAEDKGID
                     QLKQSIQQFVDVESKSILEQQMIHQLQQMDRSYVEMITEFETNKADISRRQQRLTDYK
                     DKQRLQHQLIDATLQHTDNEVEEQVYHLNARLKLQLLDDVKSVFNSQMTQNSDFNEEK
                     KVSTRVYLDQIHQRLFLEQSLITERIKKYFNKQLTEQIAPIVQQLADLHVIINPQFNF
                     ESANIEQPLLHIDFNDMLNALPKQLTKRKILNPNGQRDIHESICQSTLELLQPQMGLL
                     RQQLELYVKQMAVEAESQFESFEANIQTQINDLLAFDLDTTLINQLKDKHQQLKTILY
                     "
     gene            4751..>6011
                     /locus_tag="O0293_002265"
     CDS             4751..>6011
                     /locus_tag="O0293_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCY0281136.1"
                     /translation="MKNTFNPLQRLHFYAAIFIAPLLITLTISGIGYLFFPEVENNIY
                     KNEFFGDSDVKTHQTLNDAVHQVEQQYEGFFVSKVSILDEPYNKRITLSDMAGNQRYV
                     FLDHNNQFVADQNAKHTYSNVMRSIHSSLFTENTIINYLVELTACWMIFMILSGTYLL
                     IKKHLISNKSKALRWQKWHAMIGVIIAIPVFVLVLTGLPWSGFMGSKIAGMMDTNGAL
                     GQGELAINPPKSDLNELPWATRKNKQPASSEKGSSGHHGNAAMPQTKLDYQISIDKVV
                     EQAQKAGIKKPFSIVYPSDKNGTFIVSNTSNSGVTGLDVSPYKEQTLYFDQYSGKKLG
                     TIKYDDYGIIAKWFTWGIPLHEGHLFGILNKIINLFVCIALLVAIGMGFVSWIKRTKN
                     TAIKVPHRVKKPASISLIICLIVLGLLM"
BASE COUNT         1873 a          982 c          899 g         2257 t
ORIGIN      
        1 ttcactctga catatccaaa tacgcaaaaa gactatttct accttgtatc gatagaaata
       61 gtctttttat acttaattgg tttaattttt cataaaatct tatacttttt aaaaatggga
      121 tgaaatatat tttcctgaaa catgtaattc atgttcgtta caaattgata aaatatgatt
      181 aagcgttgtt gcaaatgaca ttttctcaaa aagcgcttca ctatcaattg gtacttgtgt
      241 gtgaatttca gccagtcgct tcgataaata tagttcatct aaattgtcat taatcttatt
      301 acgttgccca gctgataatg catcaatgtt ttcaaccaca ttttcaacgc tttgatattg
      361 ctgaattaac ttaatagccg ttttctcgcc aataccttta acacccgcat atccatctgc
      421 tgtatcaccc ataaacgctt taatatcgat tagttgttgt ggttcaaggg catattcttc
      481 gttaaaacga tgtaatgtat atctattata aatgttaaaa ccttttttaa ttagccaaac
      541 ttcaacattg tcattaatac attgcagtaa atctttgtcg cccgtaataa tatagacatc
      601 gttatcagtt gaatattgtt gtgctaatgt acctataaca tcatccgctt catagttttt
      661 aacgccaata tttacaaagc caaattgctc tgaaatttct ttaacataat caaattgtgg
      721 tatcaattct tctggtggtg cagaacgatt ttgcttataa ccatcaaaca tatcatttct
      781 aaaagttgat tgtcccatat cccaacatac agctacatgt gtagggcgta tttcatgtat
      841 tgccgaaaag atatgacgca caaatccttg tattccattt gtaggttcac cttgtgaatt
      901 gtacataaat tgtttatgaa gacttgtagc atagaaatgt ctaaataata gcgccatacc
      961 atctacaagt aatattttat taggcattta aaacgttcct tctttcttaa tataaaatag
     1021 ttttcagttg ttgatgttta tctttcaatt gattgataag tgttgtatct aaatcaaatg
     1081 ctaataaatc gtttatttgc gtttgaatat tagcttcaaa actttcaaat tgcgattcag
     1141 cttctacagc catttgcttt acatataatt caagctgttg cctcaataat cccatttgtg
     1201 gttgtaataa ttctaacgta ctttgacaaa ttgattcatg tatatctctt tgcccatttg
     1261 gattcaaaat tttacgtttt gttaattgtt taggcaatgc atttagcata tcgttgaaat
     1321 cgatgtgcaa taatggttgc tctatattag ctgattcaaa gttaaactga ggattaataa
     1381 tgacatgtaa atctgctaat tgttgaacga ttggtgcaat ttgctcagtg agttgcttat
     1441 taaagtattt ttttatacgt tctgtaatta aagattgctc taaaaacaat cgttgatgaa
     1501 tttgatctaa gtatactctc gtagacactt tcttttcttc attaaaatca ctattttgcg
     1561 tcatttgaga attaaacact gatttaacat cgtcaagtag ttgtagtttt aaacgggcat
     1621 ttaaatgata aacttgttct tcaacttcgt tgtctgtatg ttgtaacgtc gcatcaatta
     1681 attgatgttg taaacgctgt ttatctttat aatctgttaa cctttgttgg cgacgtgaaa
     1741 tatcagcttt atttgtttca aattctgtaa tcatctctac ataagaacga tccatttgtt
     1801 gaagctgatg aatcatttgt tgttctaaaa ttgatttaga ttcaacatca acaaattgtt
     1861 gtatgctttg ttttaattga tcaatgccct tatcttcagc ttgcaatgca tttcgacttg
     1921 atacagcaaa aatgtctgat tgtaagtgta cttgtcttaa tgcatctgat acatatgttt
     1981 caactgcttc aagatcatct tgactttctg ctaaatcagc agcattaatt accattttta
     2041 atgcttggtt ttcattcaac tggttcatat ctttcatgtg ttctataaac gctttgtcat
     2101 tatcagtaaa tgaatgatta aaataactta catacaatat taagtctgaa gaagttaaaa
     2161 tttgttcggt ttcatttgta tgcctttggt tatttgagtg tagccctaat gaatcaacaa
     2221 ttattttacc ttttaaccaa tcatgcatta atgcaatgtg tactgtctta acaaatgttg
     2281 catattcatc ttctgcactc cactttttca attcctgttg attaatggca tgtttttctc
     2341 cattttccaa catattgaca tacaatttat aatgtttttc aactgcctga ataaaagcga
     2401 gttgattttt atttaaatgt gattttaact tttctaaatc tgaattaata aagtcttcta
     2461 tagtcgaata agacatatct tggtattcaa ctactgcatt aatttcatct aataattgcg
     2521 attgtgattt tagcgttata taactctcgt ctccataaga tatttctgta gtagctgctg
     2581 tagtaggatt tggagaactg actaaaatat gctcgcctaa taatgcattt atcaaactac
     2641 ttttaccagc actaaatgtt ccaaatacac ctatttttat taatttatta tctatacgtg
     2701 ttaatgtttc gtggatatct tgctttgtac gcttgaacaa aggcacatcc gaaattatat
     2761 caagcgcttt ttgaatatct atagacatat ttgttgtagc tgtattttga tttagctgct
     2821 gattatcacg attatcctga gcattatcag tagccacttg atatgttgtc tcttgtcgac
     2881 ctattaattt atctagagat tcatctaaat gaatatagta gtgacgataa ttcttagtcg
     2941 tcaatgactg acgcagctca tttaattctg tataacgttg atattctttt aaatcatcac
     3001 tttcttctgt tggtaattca tctgcctgca cattttctat tatttcttta aaaattggtg
     3061 ttgattgttg ttcaacatat ttcttaatgg ctttaaccac ttcgtctgaa aatgtaagta
     3121 cataagtatt gctgattgat gtttgtggtt gatataaacc ttcaatcatt tctggcttaa
     3181 cgtcataatg ctgatttaat actttatctg aagcttcttt tttattgata aaacgcgtaa
     3241 caaatgacat atcttctcgc attggttgac gaatttgttg attaacatgt tcttgtaacg
     3301 catctgtcgc tgttaataat cgctgttgtt gaatttgtag ttttttcttc tttttattaa
     3361 aaaatccgcc aacattaaag tcttgagaca tactttccaa ataacttcgt aacatttctc
     3421 tcatattatg cggcataatg tatgcatttt ctaaaatatt tttacgctta ttctttaaaa
     3481 atgccattaa ttcatctgga ttatttaaaa gttgtgcctc ttcactaatt gcttgatgtt
     3541 gttgactatt taaaaatgct tgttcaaact ccgcttcttc gatacctaaa tcttctagta
     3601 cttcctgaat ttcagactga atgtaatcta gctgagcttc ggtaatgtat tcaaccgttc
     3661 ttgatgtata atcctctatt gtctctctat gttgatctaa tgaaattaga taacttgata
     3721 atacttcaag ttcattttca gggtgatcaa atttagatac ataaaaggtg cgttctaatt
     3781 taatacccca atctgcaatt gatttttcaa ctcgagattt aaacgtagag aatgacaatt
     3841 catcgtcttg atgcttgtca atttgattaa tgataaacac aacaggtatt ccaacatcat
     3901 ttatatgctt cataaactta aagttaagtt cagattgaac gtggttatag tcaaccgtat
     3961 aaaatatcat attacttgta tacatatatt gttctgttat tgactgatgt gatgcaacat
     4021 ttgaatcaac acctggtgta tcttgcaacg taaacccatt ttcaaattta gctgattgaa
     4081 aattaatttc tacagattca acgtcgacat tttggcgatt catttccctt acttcatcat
     4141 aattagataa tttggcatac gtttgattcg gcaaattagc aataatatcg tgattgtctg
     4201 aaactgacac aatagcagta ttacttgtcg ttggtacagg tgaacttggt aagatatctt
     4261 gttcaattaa taaatttatc agtgtcgatt tacctgcaga aaaatgtcca acgaacgaac
     4321 atgtatattg ctgcaaatat actttcttaa ttacttggtt aattgtatgt aaaagtgctt
     4381 catttcgcga cttttcaact tcttttttta atttatataa aagatctaat tgttctttat
     4441 taatcatgtt actttcccct ttggttcgtt cacatagaaa tgcttgcttg ttacaacgaa
     4501 ttcatacaat atcgatgtcc atttaaacat agatcgttaa atggctcgca ttgtattatg
     4561 tttaaatttt atcatagctg cttgataaaa catacgaata atcaattttc ttcaaacatt
     4621 ccaacttaaa agttcatttt tctaaaaatc acaaatcatc gttaaatttt tctagaaaat
     4681 aaatattgtc acagtttgtt cacacacatc acagaattga caccgtataa tagccaattg
     4741 ggagggaaat atgaaaaata catttaatcc attacaaaga ttacatttct atgcagcaat
     4801 atttattgct ccactgttaa tcactttaac catttcgggc attggttact tgttctttcc
     4861 agaagttgaa aataatattt ataagaatga gttttttggt gacagtgatg taaaaacgca
     4921 tcaaacatta aatgatgcag tacatcaagt tgaacaacaa tatgaaggat tctttgtaag
     4981 taaagttagc atacttgatg aaccatataa caaacgaatt acactgagtg atatggcagg
     5041 aaatcaacgt tacgtctttc tagatcataa caatcaattt gttgcagatc aaaatgcgaa
     5101 acatacgtat tctaatgtga tgcgaagtat acatagttct ttgtttactg aaaatactat
     5161 tattaattat ttagtagagt taaccgcatg ttggatgata ttcatgattt tatctggtac
     5221 ttatttactc attaagaagc atttaatttc taacaaaagt aaggcacttc gttggcaaaa
     5281 gtggcacgca atgattggag ttatcattgc aattccagta tttgtattag tcttaactgg
     5341 attgccatgg tctggtttta tgggcagtaa aattgccggt atgatggaca caaacggtgc
     5401 ccttggtcaa ggtgaattag cgattaatcc acctaaatca gatttgaacg aattaccttg
     5461 ggctacacgt aaaaataaac agccagcttc atccgaaaaa ggttcaagtg gtcatcatgg
     5521 taatgcagca atgcctcaaa ccaaattaga ttatcaaata tctattgata aggtcgttga
     5581 acaggcgcaa aaagctggta ttaaaaagcc gttttcaatc gtatatccaa gtgataaaaa
     5641 tggtaccttt attgtatcta atactagtaa ttcaggtgtt actgggctag atgtatcacc
     5701 atacaaggaa caaacacttt atttcgatca atatagcggt aaaaagctag gtacgattaa
     5761 atatgatgac tacggtatta ttgctaaatg gtttacatgg ggcattccgc ttcacgaagg
     5821 tcatttattc ggcattttaa ataaaatcat taatttattt gtatgtatcg ctttattagt
     5881 agccattggc atggggtttg tctcttggat aaagcgtaca aaaaatactg caataaaagt
     5941 accacatcgc gtaaaaaaac cagcatctat atcactcata atatgtttaa ttgtattagg
     6001 attattaatg c
//
LOCUS       DAKTIO010000158         5937 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.160, whole genome shotgun sequence.
ACCESSION   DAKTIO010000158 DAKTIO010000000
VERSION     DAKTIO010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5937
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.160"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            105..1043
                     /locus_tag="O0293_002266"
     CDS             105..1043
                     /locus_tag="O0293_002266"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HCY0281137.1"
                     /translation="MANIVFKLSDKDITTLMSRISFDTENLPQGMKARAKYQNTTVNI
                     YQSGKVMFQGNHAEAVSEELLPQHSQLNTNKTKKKNMANSFLEQTLMYDQFNCIGSDE
                     AGSGDYFGPLTVCAAFVTKEHVPILKTLGVDDSKKLTDTKIVELAEQLVTFIPHSLLT
                     LHNEKYNIQQAKGWTQVKMKAALHNEAIKNVLEKIDSSQLDYIVIDQFAKREVYSHYA
                     LSDIPLPKKTKFETKGESKSLAIAVASIISRYAFVTYMDQISKNINMTIPKGAGAKVD
                     VIAAKIIKKYGLSRLDTISKKHFKNREKAQKILKPL"
     gene            complement(1214..3616)
                     /locus_tag="O0293_002267"
     CDS             complement(1214..3616)
                     /locus_tag="O0293_002267"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCY0281138.1"
                     /translation="MLISNEWLKEYVTIDDSVSNLAERITRTGIEVDDLIDYTKDIKN
                     LVVGFVKSKEKHPDADKLNVCQVDIGEDEPVQIVCGAPNVDAGQYVIVAKVGGRLPGG
                     IKIKRAKLRGERSEGMICSLQEIGISSNYIPKSFESGIYVFSEAQVPGTDALQALYLD
                     DQVMEFDLTPNRADALSMIGTAYEVAALYNTKMTKPETTSNELDLSANDELTVTIENE
                     DKVPYYSARVVHDVTIEPSPIWMQARLIKAGIRPINNVVDISNYVLLEYGQPLHMFDQ
                     DAIGSQQIVVRQANEGEKMTTLDDTERELLTSDIVITNGQTPIALAGVMGGDFSEVKE
                     QTSNIVIEGAIFDPVSIRHTSRRLNLRSESSSRFEKGIATEFVDEAVDRACYLLQTYA
                     NGKVLKDRVSSGELGAFITPIDITADKINRTIGFDLSQNDIVTIFNQLGFDTEINDDV
                     ITVLVPSRRKDITIKEDLIEEVARIYGYDDIPSTLPVFDKVTSGQLTDRQYKTRMVKE
                     VLEGAGLDQAITYSLVSKEDATAFSMQQRQTIDLLMPMSEAHASLRQSLLPHLIEAAS
                     YNVARKNKDVKLFEIGNVFFANGEGELPDQVEYLSGILTGDYVVNQWQDKKETVDFYL
                     AKGVVDRVSEKLNLEFSYRRADIDGLHPGRTAEILLENKVVGFIGELHPILAADNDLK
                     RTYVFELNFDALMAVSVGYINYQPIPRFPGMSRDIALEVDQNIPAADLLSTIHAHGGN
                     ILKDTLVFDVYQGEHLEKGKKSIAIRLNYLDTEETLTDERVSKVQAEIEAALIEQGAV
                     IR"
     gene            complement(3616..4674)
                     /gene="pheS"
                     /locus_tag="O0293_002268"
     CDS             complement(3616..4674)
                     /gene="pheS"
                     /locus_tag="O0293_002268"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCY0281139.1"
                     /translation="MSEQQTMSELKQQALVDINEANDERALQEVKVKYLGKKGSVSGL
                     MKLMKDLPNEDKPAFGQKVNELRQTIQNELDERQQMLVKEKLNKQLAEETIDVSLPGR
                     HIEIGSKHPLTRTIEEIEDLFLGLGYEIVNGYEVEQDHYNFEMLNLPKSHPARDMQDS
                     FYITDEILLRTHTSPVQARTMESRHGQGPVKIICPGKVYRRDSDDATHSHQFTQIEGL
                     VVDKNVKMSDLKGTLELLAKKLFGADREIRLRPSYFPFTEPSVEVDVSCFKCKGKGCN
                     VCKHTGWIEILGAGMVHPNVLEMAGFDSSEYSGFAFGMGPDRIAMLKYGIEDIRHFYT
                     NDVRFLDQFKAVEDRGDM"
     gene            complement(5055..5795)
                     /locus_tag="O0293_002269"
     CDS             complement(5055..5795)
                     /locus_tag="O0293_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HCY0281140.1"
                     /translation="MEQITSAQNNRIKQANKLKKKRERDKTGLALIEGVHLIEEAYQS
                     GIVITQLFAIEPARLDQQIIAYAQEVFEINMKVAESLSGTVTPQGFFAIIEKPHYDIS
                     KAQQVLLIDRVQDPGNLGTLIRTADAAGMDAVIMEKGTTDPYQDKVLRASQGSVFHLP
                     VMTQDLDTFITQFNGPVYGTALENAVAYKEVTSSDSFALLLGNEGEGVNPELLAHTTQ
                     NLIIPIYGKAESLNVAIAGSILLYHLKG"
BASE COUNT         1903 a         1152 c          848 g         2034 t
ORIGIN      
        1 aattaattct ttacaacata cataaatgtt tctgacgtta ttatcattta tgatatgatt
       61 atttttgcta aagacaatga aattttatga aaggatttac acaaatggcg aatatcgttt
      121 ttaaattgtc ggataaagac ataacgacat taatgtcacg catttctttt gatactgaga
      181 atttacctca aggtatgaaa gcacgtgcaa agtatcaaaa tacaactgta aatatttacc
      241 aatcaggtaa agtcatgttt caaggtaatc atgcagaagc tgtgtctgaa gaacttctac
      301 cgcagcattc tcaattgaat actaataaaa ctaaaaagaa aaatatggct aattcttttc
      361 ttgaacaaac tttaatgtat gatcaattta actgtattgg tagtgatgag gctggaagtg
      421 gcgattactt tggtccgttg actgtgtgtg ctgctttcgt taccaaagag catgtcccta
      481 ttttaaaaac tttgggtgta gatgattcaa aaaaattaac cgataccaaa attgtagaac
      541 ttgctgagca attggttact ttcataccac attcattatt aacactacac aatgaaaaat
      601 ataatattca gcaagcaaaa ggctggactc aagtaaaaat gaaagctgcc cttcataatg
      661 aagccatcaa aaatgtactt gaaaaaattg attcgtctca attagactat atcgtcattg
      721 atcagttcgc aaaacgcgaa gtgtatagcc attacgcatt atcagatata ccacttccaa
      781 agaaaacaaa atttgaaaca aaaggagaat cgaagtcatt agccattgca gtggcaagca
      841 taatttctcg atacgcattt gttacatata tggatcaaat atcaaaaaat attaatatga
      901 cgattcctaa aggtgccggt gctaaagtcg atgtaatcgc cgccaaaatt ataaaaaaat
      961 acggtctttc acgtttagat accatttcaa aaaagcattt taaaaatcgt gaaaaagccc
     1021 aaaaaatttt aaagcctctt tgatttttaa tcattagagg cttttatgca gttgtgcgaa
     1081 gcatcatctg tattccgctg ttcctctttg actttttaaa tgcattggaa ccaaattggt
     1141 gaacccaaaa ataaaacccc atgttaggga tatcaatcta cttcagatat ccttatacat
     1201 ggggtttaaa tcattatcta ataacagcac cttgttcaat taatgctgct tcaatttccg
     1261 cttgtacttt tgaaacgcgc tcatctgtca atgtttcttc tgtgtctaaa taatttaaac
     1321 gtattgcaat tgatttttta cctttttcta aatgttcgcc ctgatataca tcaaagacaa
     1381 gtgtatcttt taatatattg ccaccatgtg catgaatcgt tgataataaa tcagctgctg
     1441 gaatattttg atctacttct aatgcaatgt cacgagacat gcctgggaat ctcggaattg
     1501 gctggtaatt aatgtaacct accgacacag ccattaatgc atcaaaattc aactcaaaaa
     1561 catacgtacg ttttaaatca ttatcagctg ctaatattgg atgtaattca ccaataaaac
     1621 caacaacttt attctctaat aagatttcag cagtacgacc tggatgtaat ccatcaatat
     1681 cagcacggcg ataactaaat tcaagattta acttttcaga tactcgatcc acgacacctt
     1741 ttgctaaata gaaatcaacc gtttctttct tatcttgcca ttgattgact acataatctc
     1801 cagttaaaat accacttaaa tattcaactt gatctggtag ttcaccttct ccattagcaa
     1861 agaagacatt gccgatttca aataatttta catctttatt tttgcgtgcc acattatatg
     1921 atgccgcttc gattaaatgt ggtaataaac tttgacgtaa tgacgcatgc gcttcactca
     1981 ttggcatcaa taaatcaatt gtttgacgct gttgcatcga aaatgcagta gcatcttctt
     2041 tagaaactaa cgaatacgta atagcttggt ctaatccagc accttctaac acttctttaa
     2101 ccattctagt tttatattgg cgatcagtta gctgaccact agtaacttta tcgaagacag
     2161 gtaacgttga tggaatatcg tcgtatccat atatacgtgc aacttcttca attaaatctt
     2221 ctttaattgt aatatcttta cgacgtgatg gtactagcac tgtaataaca tcatcattta
     2281 tttctgtatc aaaccctagt tgattaaaaa tagtaacaat atcattttgt gacaaatcaa
     2341 atccaatagt gcgattaatt ttatcagcag tgatgtcgat tggtgtaata aatgcaccaa
     2401 gttctcctga agacactcta tcttttagca cttttccgtt tgcataagtt tgtaataaat
     2461 aacatgcacg gtcgactgct tcatctacaa attcagtagc tattcctttt tcaaaacgac
     2521 tagatgattc actgcgtaaa tttaaacgtc ttgatgtatg acgaattgaa actggatcaa
     2581 aaatagcacc ttcaatcact atatttgatg tttgttcttt aacttctgaa aaatcgccac
     2641 ccataacacc agctaatgca attggagttt gtccattagt aatgacaata tcgctcgtta
     2701 ataattcacg ttctgtatca tctaatgttg tcattttttc gccttcatta gcttgacgaa
     2761 caacaatttg ttgtgaacca atcgcatctt gatcaaacat gtgcaatggt tgaccgtatt
     2821 ctaataacac ataatttgaa atgtcaacaa cattattaat aggacgtata cccgctttta
     2881 ttaagcgtgc ttgcatccaa attggcgagg gttcaattgt cacgtcgtga acaacacgtg
     2941 cactataata tggtacttta tcttcatttt caattgtcac agtcagttca tcatttgcag
     3001 ataaatcaag ctcatttgat gttgtctctg gcttagtcat ttttgtatta tataatgctg
     3061 caacttcata agcagtacct atcatactta aagcatctgc acgattcggc gttaaatcaa
     3121 attccattac ttgatcatct aaatataaag cttgtaaggc atctgttcct ggaacttggg
     3181 cttcactaaa tacataaatg cctgattcaa aacttttcgg tatatagtta cttgaaatac
     3241 caatttcttg taacgaacaa atcatacctt ctgaacgttc accgcgtaat ttggcacgct
     3301 taattttaat accaccaggc aatctgccac ctactttagc aacaatgaca tattgtcctg
     3361 catcaacgtt cggtgcacca caaacgattt gtacaggttc gtcttctccg atatcaactt
     3421 ggcaaacatt taatttatca gcatcaggat gtttctcttt tgacttaacg aagccgacaa
     3481 ctaaattttt gatatctttt gtgtagtcaa ttaaatcatc cacttcaata cctgtgcgcg
     3541 taatacgttc tgccaaatta cttacagaat catcgattgt tacatattct ttcaaccatt
     3601 catttgatat caacattaca tgtcacctct atcttctacc gctttaaatt gatctaaaaa
     3661 tctcacatca ttagtataga aatgacgaat atcttctata ccatatttca acattgcaat
     3721 acggtctggt cccataccaa atgcaaatcc agagtactct gaagaatcaa aaccagccat
     3781 ttctaataca ttaggatgta ccattccagc acctaaaatt tcaatccatc ctgtgtgttt
     3841 acacacatta caaccttttc ctttacattt aaaacatgac acatcaactt ctacagaagg
     3901 ttcagtgaat gggaagtaac ttggacgtaa acgaatttca cgatcagcac caaataattt
     3961 cttagctaac aattctaaag tacctttcaa atcactcatt ttaacgtttt tatcaacaac
     4021 taatccttcg atttgtgtaa attgatgact atgtgtcgca tcatcagagt cacgacgata
     4081 cactttacca gggcaaataa ttttaactgg accttgacca tgacgtgatt ccatcgtacg
     4141 tgcctgcact ggtgatgtat gcgtacgtaa taaaatttca tccgtaatat agaaactatc
     4201 ttgcatatca cgtgcagggt gtgatttagg taaattcagc atttcgaagt tataatgatc
     4261 ttgttcaact tcatatccat tcacaatttc ataacctaaa cctaagaata agtcttcaat
     4321 ttcttctatt gtacgtgtta atggatgctt tgaaccgatt tcaatatgac gacctggtaa
     4381 tgatacatca attgtttctt cagccaattg cttatttaat ttttctttaa ctaacatctg
     4441 ttgtctttca tctaattcat tttgaattgt ttgacgcaat tcattcactt tttgaccaaa
     4501 cgcaggttta tcttcattcg gcaaatcctt catcaatttc attagtccgc taactgaccc
     4561 ttttttacct aagtatttca ctttaacttc ttgcagtgca cgttcatcat ttgcttcatt
     4621 aatatctaca agcgcttgtt gttttaactc tgacattgtt tgttgttcag acatatgtca
     4681 ctgacctccc tattcgaact attcattttc tatttatcat atttgttgtt taactaaaaa
     4741 aagactttca tcctcttaaa aaataaggga cgaaagtcat ttccgtggta ccacccaaat
     4801 ttatgcctaa gcatacactt gtttaatatt gataacggat tgtctttccg acttaaatct
     4861 ctttaagtaa ccaaaaaggt gaaaagactt taaataattg gaataacttg cagtcacgat
     4921 tattctccct aaaacaatta tttatataaa tgtccatgcc tttttattgg tttatattca
     4981 attgtgtatg cattcacgta gaaatgttct gttaaacaat taattataat tatatcggaa
     5041 aactttcaac acggtcaacc tttcaaatga taaagtaaaa tactacctgc aatcgctaca
     5101 tttaaacttt cagctttacc ataaataggt atgattaaat tttgtgtagt atgtgctaat
     5161 aattcaggat taacaccttc tccctcatta cctaatagta atgcaaaaga atcacttgaa
     5221 gtaacttctt tgtatgccac tgcgttttca agtgctgtac cataaacagg accattaaat
     5281 tgagtaataa acgtatcgag atcttgtgtc ataactggca aatggaaaac actaccttga
     5341 ctcgctcgca acactttatc ttgataagga tctgtcgtac ccttctccat tattacagca
     5401 tccattccag cagcatccgc agttctaatt aatgtgccta aatttccagg atcttgaaca
     5461 cgatcgatga gcaatacttg ttgtgcttta gaaatatcat aatgcggctt ctcaatgatt
     5521 gcgaaaaacc cttgtggtgt cactgtacct gataaagatt cagcaacttt catgtttatt
     5581 tcaaaaactt cttgcgcgta tgcgataatt tgctgatcta atcttgccgg ttcaattgca
     5641 aataattgtg taattacaat tccactttga taagcttctt caattaaatg cacaccttca
     5701 attaaagcta atccagtttt atccctctca cgtttctttt ttagcttgtt cgcttgttta
     5761 attctattat tttgtgcaga agtaatttgt tccattgata gctcctcgct ttatttttaa
     5821 aaataaaaat attaatcatt aataagatga aaacatttga ttgtatagtt aatattaatt
     5881 aatcgctttt atcactcata atatttcaaa ttgtataaat ttcttttatc gatacta
//
LOCUS       DAKTIO010000159         5886 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.161, whole genome shotgun sequence.
ACCESSION   DAKTIO010000159 DAKTIO010000000
VERSION     DAKTIO010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5886
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.161"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            241..444
                     /locus_tag="O0293_002270"
     CDS             241..444
                     /locus_tag="O0293_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCY0281141.1"
                     /translation="MTFYNFIMGFQNDNTPFGILAEHVSEDKAFPRLEERHQVIRAYV
                     MSNYTDHQLIETTNRAISLYMAN"
     gene            complement(441..677)
                     /locus_tag="O0293_002271"
     CDS             complement(441..677)
                     /locus_tag="O0293_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281142.1"
                     /translation="MDNHNQCNYVNPQNVSLDWECFIISKSEMLLDGVPNELINTWLD
                     KDIITPFSIRNDEINFKTKDIWDALIHHNWYYSN"
     gene            902..1483
                     /locus_tag="O0293_002272"
     CDS             902..1483
                     /locus_tag="O0293_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCY0281143.1"
                     /translation="MAKTLYIMRHGQTLFNFKGLIQGFGDSPLTELGIAQAQKARSYY
                     ETKGINFDLYASSTQERASDTLENVAPNQSYQRFKGLKEWHFGIFEGESVYLFDNLYK
                     PEDLFGDRIVPFKGEARQQVEERIVKTLHDIMSQTKNNALVVSHGTIMGVFLRYCLKL
                     DEALKHNIGNCNILKFEYDNGTFKFVELIDPNL"
     gene            complement(1556..2173)
                     /locus_tag="O0293_002273"
     CDS             complement(1556..2173)
                     /locus_tag="O0293_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HCY0281144.1"
                     /translation="MVTAIIHGFILAIGLILPLGAQNVFIFNQGANQPKYRYVLPAII
                     TAGLSDSLLIIIAVVGVSIIIMSLPVLQAIIYIVGLIFLMYMAWTIWHDKPSTDGETQ
                     IMSPMKQVSFALSVSLLNPHAILDTIGVIGSSAALYSGSNKIAFTIACISVSWLWFFL
                     LAILGKMVGSIDKTGKLLTIINKISSIIIIIVALMILQKLIQLLF"
     gene            complement(2328..2822)
                     /locus_tag="O0293_002274"
     CDS             complement(2328..2822)
                     /locus_tag="O0293_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0281145.1"
                     /translation="MIEIKTLTNNDFNEYKRLVSTVNEEFTQDSHYSQTMTDTLIHDI
                     LNQGSPKCIVFGCYENETLIATAALEQIRYVGKEHKSLIKYNFVTNNDKSINSELINF
                     IINYARQNNYESLLTSIVSNNIGAKVFYSALGFDILGFEKNAIKIGNTYFDEHWLFYD
                     LINK"
     gene            complement(3221..3643)
                     /locus_tag="O0293_002275"
     CDS             complement(3221..3643)
                     /locus_tag="O0293_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCY0281146.1"
                     /translation="MAIHYETKATNVGGRKGHVYTDDRALDIDIVPPAQADGKATNPE
                     QLFAAGYASCFNGAFDLILKQNKVRDAHPEVTLTVRLEDDSDSESPKLSVSIDATIKN
                     VISQEEAEKYLQMAHEFCPYSKATQGNINVDLNVNVVD"
     gene            3791..4507
                     /locus_tag="O0293_002276"
     CDS             3791..4507
                     /locus_tag="O0293_002276"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCY0281147.1"
                     /translation="MTHVEVVATIAPQLSIEETLIQKINHRIDAIDVLELRIDQIENV
                     TVDQVAEMITKLKVMQDSFKLLVTYRTKLQGGYGQFTNDSYLNLISDLANINGIDMID
                     IEWQADIDIEKHQRIITHLQQYNKEVVISHHNFESTPPLDELQFIFFKMQKFNPEYVK
                     LAVMPHNKNDVLNLLQAMSTFSDTMDCKVVGISMSKLGLISRTAQGVFGGALTYGCIG
                     VPQAPGQIDVTDLKAQVTLY"
     gene            4590..5129
                     /locus_tag="O0293_002277"
     CDS             4590..5129
                     /locus_tag="O0293_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HCY0281148.1"
                     /translation="MELQQAIANRRSVKKFKRDMHIDDALLYQAIEKAADAPNHGMRE
                     PWRVVHVPKDRLGDMSKDISKFAFPNELDKQQCHYDAVTKLGGMLLLILKTDPRQRQN
                     DENYFAFGAYAQNLMLLLYEAGIGTCWKSPLYIYDPKVRKTLGIKKDEVLAGFLYLTD
                     LEGDMPKAPRKNRNLITLY"
     gene            complement(5279..5599)
                     /locus_tag="O0293_002278"
     CDS             complement(5279..5599)
                     /locus_tag="O0293_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCY0281149.1"
                     /translation="MQSIKSNESFKSVINSDTPVIVKFEAGWCPDCRAMDLWIDPIVE
                     QYNDYQWYTVNRDELEDVVVENEVMGIPSLLVFKNGDKIAHLHSANAKSPEQVESFLA
                     ETFK"
     gene            5743..>5886
                     /locus_tag="O0293_002279"
     CDS             5743..>5886
                     /locus_tag="O0293_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HCY0281150.1"
                     /translation="MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKT
                     IIAN"
BASE COUNT         2105 a          859 c          936 g         1986 t
ORIGIN      
        1 gcagaatgct aaagtttatt aattcaaaca agtgagcata tataggggtg tattgtatta
       61 atatgaaaag aatatttaca tactaaaaac tcttaaagtt gtataattcg ttaatatttt
      121 tagaatcaga tatcaaaaaa atgtgcaaaa tcacttttgc acatttacaa atactagttt
      181 tctgataaga ttaataataa cactttaaat agcgcttaat aaatgaaggg ggcaagtctt
      241 atgacgtttt acaatttcat catgggtttt caaaatgata acacaccatt tggtatattg
      301 gccgaacacg ttagtgaaga taaagcattc cctcgattag aagaaagaca ccaagtaatt
      361 agagcatatg tgatgtctaa ttacacagat catcaattaa ttgaaactac aaatagagct
      421 attagcttat atatggcaaa ttaatttgag tagtaccaat tatgatgtat tagtgcatcc
      481 caaatatctt ttgttttaaa gtttatttca tcatttctta tcgaaaatgg tgtaataatg
      541 tctttatcta accaagtgtt gataagttca tttggtacac catctaacaa catttcactt
      601 ttactaatta taaaacattc ccagtcaagt gaaacatttt gtggattcac ataattacat
      661 tgattatgat tatccataaa cactcactcc tttaaaattc tgtactcttc attgcgtttt
      721 accccgtcac attatctttt aaactaaaat catcattact tatgaaaaaa atgtacatca
      781 aaagcaaagg ttttcgctac cgaaaaagtt taaataatgg tttaatatat ttggtactca
      841 ttttaataaa aagagaatac attttgagct atcaatactt tttattgaag aggtgttatt
      901 gttggctaaa acgttatata taatgcgcca cggacaaact ttgtttaatt ttaagggact
      961 aattcaggga tttggagatt cgccgctaac agaacttgga attgctcaag ctcaaaaggc
     1021 acgtagttat tatgaaacta aggggataaa cttcgattta tatgcatcat caacgcaaga
     1081 acgcgcaagt gacacacttg aaaatgttgc acctaaccaa tcgtatcaac gttttaaggg
     1141 actgaaggaa tggcattttg gaatatttga aggtgagtca gtctatctat ttgataatct
     1201 atacaagcct gaggacttat tcggagatcg aattgttcct ttcaaaggag aggcaaggca
     1261 acaagttgaa gaacgcattg tgaaaacttt acatgacatt atgtctcaaa caaagaataa
     1321 tgcattagtc gtgagtcatg gaacaataat gggagtattt ttaagatatt gccttaaact
     1381 agatgaagca ttaaagcata atatcggtaa ttgtaatatc ctgaaatttg aatatgacaa
     1441 tggaacattt aaatttgttg agttaattga tccaaattta taataaataa agcgttatac
     1501 atagacacaa gtatgtcttt tgtataacgc ttttaaaata caactacata acgctttaga
     1561 ataataattg aataagtttt tgtaatatca tcagagcaac aataataatt attatgcttg
     1621 atattttatt gattattgtt aataattttc cggttttatc aattgatcca accatttttc
     1681 ctaaaattgc gagtaaaaag aaccataacc atgaaacgct aatacaagcg attgtaaacg
     1741 caattttatt gctgccacta tataatgcag cactactacc aattactcca attgtatcta
     1801 aaatagcatg tggattgagt aatgaaactg ataaagcaaa acttacttgt ttcattggag
     1861 acataatttg agtttctcca tctgttgagg gtttatcatg ccaaatggtc caagccatat
     1921 acatcaagaa aattaaacca actatataaa taattgcttg aagtacaggt aaagacataa
     1981 taatgataga tactcctacc actgcaataa taataagtaa gctgtctgac aacccggctg
     2041 taattattgc aggcaataca tatctatatt ttggttgatt agctccttgg ttaaaaataa
     2101 atacattttg tgcacctaaa ggtaaaatta gaccaatcgc taagataaat ccatgaataa
     2161 ttgcggttac cattaagttt tgacacccct aaaacataga atattctgaa aaaacttatt
     2221 gttatcatat caaataaaaa acaactactc aatagtagag tagttgtatg aaaaatagaa
     2281 cctatatgat ttgaatttat gagaaatgta gaatatataa aactgtatta cttattaatc
     2341 aaatcataaa aaagccaatg ttcatcgaaa taggtatttc cgattttaat tgcatttttc
     2401 tcaaaaccaa gaatgtcgaa tcctagtgca ctatagaaaa ctttagcacc tatgttgttt
     2461 gacacaattg atgtaagtaa agattcgtaa ttgttctgcc gtgcataatt aataatgaaa
     2521 ttaatgagct cgctattaat cgatttatcg ttattagtaa caaagttgta tttaattaat
     2581 gatttatgtt cttttccaac gtatcgaatt tgttctaagg cagctgttgc gataagtgtt
     2641 tcgttttcat aacagccaaa tacaatacat ttcggtgaac cttgatttaa aatgtcatgt
     2701 attaaggtgt cagtcattgt ttgactataa tgtgaatctt gagtgaattc ttcattgact
     2761 gtcgaaacaa gtctcttata ctcattaaaa tcattattcg ttaatgtttt aatttcaatc
     2821 attactgacc tccttaatca ttattaagaa agattttgta ctaaaaaaat ttcttaatta
     2881 catattaaaa tgtttattaa aattttgcaa tcactgattt atatgcacat tcttgtaaat
     2941 ggattatcaa tttaattata tttattttta agttcaggaa attatggaat tggttattgg
     3001 tcgacactgg acttttattt gtagacggta tgcgaagaag tcaagccagt attggaaccg
     3061 gtcacattta tgatgactaa ggtacaatca ttttcagtta aatattgcca gaaatttcaa
     3121 tcacgaatag aaaaaataac caaccaaaaa tttttggttg gcaaatttga attttatgtg
     3181 aattttatta ataacgttga ataatctctt taagttaatg ctaatctaca acatttacat
     3241 ttaaatcgac attaatattt ccttgagtcg cttttgaata tggacaaaat tcatgagcca
     3301 tttgtaaata tttttcagct tcttcttgag atataacatt tttaattgtc gcatcaattg
     3361 aaacacttaa tttaggactt tctgagtctg aatcatcttc tagtctcact gttagtgtta
     3421 cttctggatg agcatcacgc actttgtttt gctttaaaat taggtcgaaa gcaccgttga
     3481 agcaagatgc ataacctgct gcaaataatt gttcggggtt agtagcttta ccatctgctt
     3541 gagcaggcgg aacgatatca atatctaatg ctcgatcatc agtataaaca tgtcctttac
     3601 gtccgccaac attcgtcgct ttagtttcat aatgtattgc catacttata acctcctaat
     3661 ttaaaataca tttatgcttt acccattcga aaatacctta atcattttca tttatagcat
     3721 tgtttgattg aaggataaaa agttgttgtt tacaataaaa ataatgagta tctgaaatga
     3781 gggattcact atgacacatg tggaagtagt agcgactatc gcgccacaat tatctatcga
     3841 agaaacttta attcaaaaaa ttaatcatcg tattgatgca atagacgtat tagaattacg
     3901 aattgatcaa attgaaaatg tcacagttga tcaagtggca gaaatgatta caaagctgaa
     3961 ggttatgcaa gattcattca aattattagt tacgtatcgt acaaagttac aaggtggcta
     4021 tgggcaattt acaaatgact cgtatcttaa tttaatatca gacttagcaa atatcaatgg
     4081 catagatatg attgatatag aatggcaagc agatattgac attgaaaaac atcaacgaat
     4141 cattacacat ttgcaacagt ataataaaga ggtggttata tcacatcata atttcgaaag
     4201 tacgcctcca ttagatgaat tgcaatttat attttttaaa atgcaaaaat tcaacccaga
     4261 atacgttaaa ttagcagtaa tgccacataa taaaaatgat gtgttaaatt tattgcaggc
     4321 aatgtctaca ttttcagata ctatggactg caaagttgtt ggtatttcaa tgtctaaact
     4381 tggactaata agtagaacgg ctcaaggcgt ttttggtggt gcgttgactt atggttgtat
     4441 cggagtacca caagctccag gacagattga tgttactgat ttaaaagcac aagtgacttt
     4501 atactaataa aatgaattag gttgcttttg tgtaagtgct gtcttataat tgagaagtat
     4561 tatcattgtt cgtggaggag cgattttaga tggaattaca acaagcaata gctaatagaa
     4621 gaagtgtgaa aaaatttaaa agagatatgc acatagatga cgcattgcta tatcaagcaa
     4681 ttgagaaagc tgctgatgct ccaaatcacg gaatgaggga accatggaga gttgtgcatg
     4741 ttccgaaaga cagattagga gatatgagta aggatatttc taaatttgca tttcctaatg
     4801 aattagataa gcaacaatgt cattatgatg cagttacgaa actaggtggc atgttattgc
     4861 ttattttaaa aacagatcca agacaacgtc aaaatgatga aaactacttt gcatttggtg
     4921 catatgcaca aaatcttatg ttgttacttt atgaagcggg aataggtaca tgttggaaat
     4981 cgccattata tatctatgat cctaaagtaa gaaaaacact tggtataaag aaagatgaag
     5041 ttcttgctgg attcttatat ttaacggatt tagaaggaga tatgcctaaa gcaccacgta
     5101 aaaatagaaa cttaattaca ttatattaat atgtataatt atagaaacat taataaaagc
     5161 tgagtcatga attgatggac atctatcgag ttagagattt aatctaactt actagagtcg
     5221 gtacaataac agtctcagct ttttattgtg cagtatatac acatttttat tttagtattt
     5281 atttaaaagt ttctgctaaa aatgattcaa cttgttcagg tgacttagca tttgctgaat
     5341 gaaggtgtgc aattttatcg ccgtttttaa atactagcaa gctagggata cccataactt
     5401 cattttcaac aactacatct tctaattcat cacgattaac agtataccat tggtaatcat
     5461 tatattgttc tacgattggg tcaatccata aatccatagc acgacagtct gggcaccatc
     5521 ctgcctcaaa tttaacaatt acaggtgtat cgctattaat tacagattta aatgattcat
     5581 tacttttgat tgattgcatt gtaacaactc ctctagatag tttaataatt tttattatag
     5641 ctaaatttat atcataataa aaaattttag cttcaaaatg aaagcctttt gctttggaaa
     5701 atgatatatt tattttaaat acataaagga ggttgcagtc gtatgattaa attttaccaa
     5761 tataagaatt gtacaacttg taaaaaggca gcaaagtttt tagatgaata tggcgtaagt
     5821 tatgaaccaa ttgatatcgt tcaacataca cctacaataa atgaatttaa aacaataatt
     5881 gcaaat
//
LOCUS       DAKTIO010000160         5879 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.162, whole genome shotgun sequence.
ACCESSION   DAKTIO010000160 DAKTIO010000000
VERSION     DAKTIO010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.162"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..190
                     /locus_tag="O0293_002280"
     CDS             <1..190
                     /locus_tag="O0293_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HCY0281151.1"
                     /translation="SIRETKVGYFLAKIYEPFLQPFRKVIPPIGIIDISSIAAIIVLV
                     LFQKGLLQIFNWILIQLQ"
     gene            300..1079
                     /locus_tag="O0293_002281"
     CDS             300..1079
                     /locus_tag="O0293_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCY0281152.1"
                     /translation="MINIDIYQHFRQEEYELIDQLTDKCDQAEQHYAPVLTHFLDPRG
                     QYILEVICGSYEDLNVSFYGGPNAERKRAIISPNYYEPKESDFELTLMEIDYPEKFVT
                     LKHQHILGTLMSLGIEREQVGDIIVNERIQFVLTSRLESFIMLELQRIKGASVKLYTI
                     PVTDMIQSNENWKNESATVSSLRLDVVIKEMIRKSRTIAKQLIEKKRVKVNHTIVDSA
                     DFQLQANDLISIQGFGRAHITDLGGKTKKDKTHITYRTLFK"
     gene            1103..1720
                     /locus_tag="O0293_002282"
     CDS             1103..1720
                     /locus_tag="O0293_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HCY0281153.1"
                     /translation="MPFTPNEIKNKEFSRVKNGLEPTEVANFLEQLSTEIERLKEDKK
                     QLEKVIEERDTNIKSYQDVHQSVSDALIQAQKAGEETKQAAEKQAEAIIAKAEAQANQ
                     MVGDAVEKARRLAFQTEDMKRQSKVFRSRFRMLVEAQLDLLKNEDWDYLLNYDLDAEQ
                     VTLENIHHLHENDLKPDEVAANAQNNASNTPDNNQQSNDSETTKK"
     gene            1941..4694
                     /gene="ileS"
                     /locus_tag="O0293_002283"
     CDS             1941..4694
                     /gene="ileS"
                     /locus_tag="O0293_002283"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCY0281154.1"
                     /translation="MDYKETLLMPKTDFPMRGGLPNKEPQIQEKWDAEDQYHKALEKN
                     KGNETFILHDGPPYANGNLHMGHALNKILKDFIVRYKTMQGFYAPYVPGWDTHGLPIE
                     QALTKKGVDRKKMSTAEFREKCKEFALEQIELQKKDFRRLGVRGDFNDPYITLKPEYE
                     AAQIRIFGEMADKGLIYKGKKPVYWSPSSESSLAEAEIEYHDKRSASIYVAFDVKDDK
                     GVVDADAKFIIWTTTPWTIPSNVAITVHPELKYGQYNVNGEKYIIAEALSDAVAEALD
                     WDKASIKLEKEYTGKELEYVVAQHPFLDRESLVINGDHVTTDAGTGCVHTAPGHGEDD
                     YIVGQKYELPVISPIDDKGVFTEEGGQFEGMFYDKANKAVTDLLTEKGALLKLDFITH
                     SYPHDWRTKKPVIFRATPQWFASISKVRQDILDAIENTNFKVNWGKTRIYNMVRDRGE
                     WVISRQRVWGVPLPVFYAENGEIIMTKETVNHVADLFAEHGSNIWFEREAKDLLPEGF
                     THPGSPNGTFTKETDIMDVWFDSGSSHRGVLETRPELSFPADMYLEGSDQYRGWFNSS
                     ITTSVATRGVSPYKFLLSHGFVMDGEGKKMSKSLGNVIVPDQVVKQKGADIARLWVSS
                     TDYLADVRISDEILKQTSDVYRKIRNTLRFMLGNINDFNPDTDSIPESELLEVDRYLL
                     NRLREFTASTINNYENFDYLNIYQEVQNFINVELSNFYLDYGKDILYIEQRDSHIRRS
                     MQTVLYQILVDMTKLLAPILVHTAEEVWSHTPHVKEESVHLADMPKVVEVDQALLDKW
                     RTFMNLRDDVNRALETARNEKVIGKSLEAKVTIASNDKFNASEFLTSFDALHQLFIVS
                     QVKVVDKLDDQATAYEHGDIVIEHADGEKCERCWNYSEDLGAVDELTHLCPRCQQVVK
                     SLV"
     gene            4984..5781
                     /locus_tag="O0293_002284"
     CDS             4984..5781
                     /locus_tag="O0293_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0281155.1"
                     /translation="MYHNSNANFVNGITLNVRDKNELKPFYEDILGLNIINETLTSIQ
                     YEVGQNNHVITLVELQNGREPLMSEAGLFHIAIKLPQISDLANLLIHLSEYDIPVNGG
                     IQPASLSLFFEDPEGNGFKFYVDKDEAQWTRQNNLVKIDIRPLNVPRLVSHATKLLWL
                     GIPDDAIIGALHIKTIHLSEVKEYYLDYFGLEQSAYMDDYSIFLASNGYYQHLAMNDW
                     VSATKRVENFDTYGLAIVDFHYPETTHLNLQGPDGIYYRFNHIEVED"
BASE COUNT         2136 a          830 c         1120 g         1793 t
ORIGIN      
        1 aagtattaga gaaactaagg taggttattt tttagcgaaa atatatgaac ctttcttaca
       61 accatttaga aaagtaattc cacctattgg aattatcgac atatcatcaa tcgctgcaat
      121 tatcgtttta gtattattcc aaaaagggtt actccaaatc tttaattgga ttttaattca
      181 attacaataa attaagcgac ataacgaggc aagggtacat gatactttag cctcgttttt
      241 gatatgcatt tttctgaata taagggcaat agatggtatt ttataatttt tttaaggtag
      301 tgattaacat agatatttat caacacttta gacaggaaga atacgaatta attgatcagc
      361 taacggataa atgtgatcaa gcggaacagc attatgcacc agtattaacg cattttttag
      421 atccaagagg gcaatatata ttggaagtga tttgtggcag ttatgaagat ttaaacgtat
      481 ctttttatgg tggacctaat gctgaaagaa aaagagcaat catttcgccg aactattatg
      541 aacctaaaga aagcgacttt gaattaactt taatggaaat agattatcct gaaaaattcg
      601 tcactttaaa acatcaacat attttaggga cattaatgtc tttaggtatc gaacgcgaac
      661 aagttggaga tataattgtg aatgaacgaa ttcaatttgt tttgacaagt agattggaat
      721 catttattat gttagaatta caacgtatta aaggcgcatc agttaaactt tatactattc
      781 cagtaacaga tatgatacaa tctaatgaga attggaaaaa tgaaagtgca acagttagtt
      841 ctttaaggtt agatgttgtt attaaagaaa tgatacgtaa atcacgtacg attgcgaaac
      901 aactaatcga aaaaaaacgt gttaaagtga atcacactat tgttgattca gcagattttc
      961 aattacaagc aaatgattta atatccatcc aaggttttgg tagagcacac attactgact
     1021 taggtggtaa aactaaaaaa gataaaacgc acattaccta tagaacatta ttcaaatagt
     1081 aatgatttaa ggaggataac aaatgccttt tacaccaaat gaaattaaga ataaagagtt
     1141 ttcacgtgta aagaatggtt tagaacctac tgaagttgct aattttttgg agcaactaag
     1201 cactgaaatt gaacgtctta aagaagataa aaaacaactt gaaaaagtaa tcgaagagag
     1261 agatactaat attaagtctt atcaagacgt gcatcaatct gtaagtgatg ctttgataca
     1321 agctcaaaaa gctggtgaag aaactaagca agctgcagag aaacaagctg aagcgattat
     1381 agctaaggca gaagcgcaag ctaatcaaat ggttggtgac gcggtagaaa aagcacgccg
     1441 tttagcattc cagactgaag atatgaaacg tcaatcaaaa gtatttagat cgcgtttccg
     1501 tatgttagtt gaagcgcaat tagacttatt aaaaaacgaa gattgggatt acttgttgaa
     1561 ttatgattta gacgctgaac aagtgacgct tgaaaatatt catcatttgc atgaaaatga
     1621 tttaaagcca gatgaagttg cagcaaatgc acaaaataat gcatcaaata caccagacaa
     1681 taatcaacaa tccaatgatt cagaaacaac taagaagtaa gaattaaata aagacagacg
     1741 cgtaatatac atttaacttt tcacagcgaa ttaggtaatg gtgagagcct agtaaaagca
     1801 tgtatgttat atcactggct ttttaatatt taaataatgt aatgagagaa ctctaagttg
     1861 agttaataag ggtggtaccg cgagcaatcg tcccttttaa tttaacttag agttttttaa
     1921 atttttaagg agtgaaaaaa atggattaca aagaaacgtt attaatgcct aaaacagatt
     1981 tcccaatgcg aggtggttta ccaaacaagg aaccgcaaat tcaagaaaaa tgggatgcag
     2041 aagatcaata ccataaagcg ttagaaaaaa ataaaggtaa cgaaacattc attttacatg
     2101 atggcccacc atacgcgaat ggtaacttac atatgggaca tgccttgaac aaaattttaa
     2161 aagactttat tgtacgttat aaaactatgc aagggttcta tgcaccatac gtaccaggtt
     2221 gggatacaca tggtttacca attgaacaag cattaacgaa aaaaggtgtt gaccgaaaga
     2281 aaatgtcaac agctgaattc cgtgagaaat gtaaagaatt tgctttagaa caaattgaat
     2341 tacagaaaaa agattttaga cgtttaggtg ttcgtggtga ctttaatgat ccatatatta
     2401 cattaaaacc tgaatacgaa gctgcacaaa ttcgtatttt tggagaaatg gcagataaag
     2461 gtttaattta taaaggtaaa aagccagttt attggtctcc ttcaagtgag tcttcattag
     2521 cagaagcaga aattgaatat cacgataaac gttcagcatc aatttacgtt gcatttgacg
     2581 ttaaagatga caaaggtgtc gttgatgcag atgctaaatt tattatctgg acaacaacgc
     2641 catggacaat tccatcaaat gttgcgatta ccgttcatcc tgaattaaaa tatggtcaat
     2701 acaatgtaaa tggcgaaaaa tatattattg cagaagcctt gtctgacgct gtagcagaag
     2761 cactggattg ggataaagca tcaatcaaat tagaaaaaga atacacaggt aaagaattag
     2821 agtatgttgt agcacaacat ccattcttag acagagaatc gttagtgatt aatggtgatc
     2881 atgttactac agatgctggt acaggttgtg tacatacagc accaggtcac ggggaagatg
     2941 actatattgt tggtcaaaaa tatgaattgc cagtaattag tccaatcgat gataaaggtg
     3001 tatttactga agaaggcggc caatttgaag ggatgttcta tgataaagct aataaagccg
     3061 ttactgattt attaacagaa aaaggtgcac tattaaaatt agactttatt acacatagct
     3121 atccacacga ctggagaaca aaaaaacctg taatcttccg tgctacacca caatggtttg
     3181 cctcaatcag taaagtaaga caagatattt tagatgcaat cgaaaataca aacttcaaag
     3241 taaattgggg taaaacacgt atttacaata tggttcgtga ccgtggcgaa tgggttattt
     3301 ctcgtcaacg tgtgtggggt gtaccgttac cagtatttta tgctgaaaat ggcgaaatta
     3361 tcatgacgaa agaaacagtg aatcatgttg ctgatttatt tgcagaacac ggttcaaata
     3421 tttggtttga aagagaagcg aaagacttac taccagaagg atttacacat ccaggcagcc
     3481 ctaacggtac atttactaaa gaaacagaca ttatggacgt ttggtttgat tctggttcat
     3541 cacaccgtgg cgtgttggaa acaagaccgg aattaagttt cccagcggat atgtatttag
     3601 aaggtagtga ccaatatcgt ggttggttca actcttctat cacaacttca gttgctacaa
     3661 gaggagtatc accttataaa ttcttacttt ctcatggttt tgttatggac ggtgaaggta
     3721 agaaaatgag taaatcttta ggtaatgtga ttgtacctga ccaagtggtt aaacaaaaag
     3781 gtgctgatat tgcgagactt tgggtaagta gtacggacta tttagctgat gttagaattt
     3841 ctgatgaaat tttaaaacaa acatctgatg tttatcgtaa aatcagaaat acattaagat
     3901 ttatgttagg taacattaac gatttcaatc ctgacacaga tagcattcct gaatcagagt
     3961 tattagaagt ggatcgttac ttgctaaatc gtttacgtga atttactgca agtacgatta
     4021 acaactatga aaactttgac tacttaaata tttatcaaga agttcaaaac tttatcaatg
     4081 ttgagttaag taatttctat ttggattacg gtaaagatat tttatatatt gaacaacgtg
     4141 attctcatat ccgtcgtagt atgcaaacag tgttatatca aattttagtt gatatgacga
     4201 agttgttagc accaatctta gtgcatacag ctgaagaagt ttggtctcat acaccacatg
     4261 ttaaagaaga aagtgttcac ttagcagaca tgcctaaagt tgtagaagta gatcaagctt
     4321 tattggataa atggcgtaca tttatgaatt tacgtgatga tgtgaaccgt gcattagaaa
     4381 ctgctcgtaa tgaaaaagtt attggtaaat cattagaagc taaagttacg attgctagta
     4441 acgataaatt taatgcatct gaattcttaa cttcatttga tgcattacat caattattta
     4501 tcgtgtcaca agttaaagtt gtagataagt tagacgatca ggcaacagct tatgaacatg
     4561 gtgatattgt catcgaacat gcagatggtg aaaaatgtga aagatgttgg aactattcag
     4621 aggatcttgg tgctgttgat gaattgacgc atctatgtcc acgatgccaa caagttgtaa
     4681 aatcacttgt ataattgaaa ttgtataaag tactcataca gatgatataa attaaagctc
     4741 tcttcataat catgttgtag tttttgttga catgatgaag agagtttttt tgtgaataaa
     4801 aaaatgacca agttaccggt catatatgta aaaaatgtgc gatttactaa aataaaaatt
     4861 attcaggaat ggtacaaatt ctctgaggca tataaatgcg ttatagttgc tattctcaat
     4921 tatgttcgcg ataattttaa gtaaaagtaa gcacagatat tgaatttgat aggagttaat
     4981 tgaatgtatc ataacagtaa cgcaaacttt gtcaatggta tcactttaaa tgtgagagat
     5041 aagaatgaat taaagccatt ttatgaggac atattaggat taaatattat aaatgagaca
     5101 ttaacatcga tacaatatga agtaggtcaa aataatcatg tcattacact tgttgaatta
     5161 caaaatggac gtgaaccttt aatgtccgaa gcgggactgt ttcatatcgc aattaaacta
     5221 cctcaaatta gtgatttagc taatttacta attcatttaa gcgaatatga tattccagtt
     5281 aacggaggta tacagcctgc ttcgttatca ttattttttg aagacccgga aggaaacggt
     5341 tttaaatttt atgttgataa agacgaagcg caatggacga ggcaaaataa tttagtaaaa
     5401 attgatatta gaccattaaa tgtaccgaga ttagtgagtc atgcaacaaa attgttatgg
     5461 ttaggtattc cagatgacgc tattataggt gcattgcata ttaagacaat tcatttatca
     5521 gaggtaaaag agtactacct cgattatttt ggattagagc aatcggcata tatggatgat
     5581 tattcaatat ttttagcatc gaatggctat tatcaacatt tggccatgaa tgattgggta
     5641 tcagcaacga aacgtgtaga aaattttgat acgtatggat tagcaattgt tgactttcat
     5701 tatcctgaaa caacacattt aaatttacaa ggtccggatg gtatctatta tcgctttaat
     5761 catatcgaag ttgaagatta gtatatactt tgaatggacg aaccatataa tgaatcgttt
     5821 ttaatgatct ttttatacaa gttatgaagg aggctgggac attaagttct taggcaatg
//
LOCUS       DAKTIO010000161         5858 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.163, whole genome shotgun sequence.
ACCESSION   DAKTIO010000161 DAKTIO010000000
VERSION     DAKTIO010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5858
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.163"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..2395
                     /gene="mnhA1"
                     /locus_tag="O0293_002285"
     CDS             <1..2395
                     /gene="mnhA1"
                     /locus_tag="O0293_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit A"
                     /protein_id="HCY0281156.1"
                     /translation="HIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIYMLTL
                     IKTTMSGNTVMKTLNWMPHFGMNFDLYLDGLGLLFSLLISGIGSLVVLYSIGYLSKSE
                     QLGNFYCYLLLFMGAMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIYGAQK
                     SLIITVFGGLSLLGGIILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIGAFTK
                     SAQFPFYIWLPDAMEAPTPVSAYLHSATMVKAGLYLIARMTPIFAASQGWVWTVTLVG
                     LITLFWASLNATKQQDLKGILAFSTVSQLGMIMAMLGIGAISYHYQGDDSKIYAAAFT
                     AAIFHLINHATFKGALFMITGAVDHSTGTRDVKKLGGLLTIMPISFTITVITALSMAG
                     VPPFNGFLSKESFLETTFTASQANLFSVDTLGYLFPIIGIVGSVFTFVYSIKFIMHIF
                     FGQYKPEQLPKKAHEVSILMLLSPAILATLVIVFGLFPGILTNSIIEPATSSINHTVI
                     DDVEFHMFHGLTPAFLSTLVIYILGILLIVTFSYWVKLLQRQPGKLTFNYWYNRSANV
                     IPNYSEKMTNSYVTDYSRNNLVIIFGALILLTFVTIFSVPFNINFKDVSPIRIFEVCI
                     VILLLSAAFLILFAKSRLFSIIMLSAVGYAVSVLFIFFKAPDLALTQFVVESISTALF
                     LLCFYHLPNLNRYNEKRSFQLTNALIAGGVGLSVIIIGLIAYGNRHFESISKFYQEHV
                     YDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAVYTMIKLRKKRQTQGNEVKNHE"
     gene            2388..2816
                     /gene="mnhB1"
                     /locus_tag="O0293_002286"
     CDS             2388..2816
                     /gene="mnhB1"
                     /locus_tag="O0293_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit B"
                     /protein_id="HCY0281157.1"
                     /translation="MNRQQNDLILQFAAVIIFFMVMVFGFSLFLAGHYTPGGGFVGGL
                     LFASSLVIITIAFDIETMRKIFPLDFKILIGIGLVFCIATPIASWFLGKNFFTHVTFD
                     IPLFILEPVHMTTAVFFDFGVLCAVVGTVMTIIISIGENE"
     gene            2816..3157
                     /gene="mnhC1"
                     /locus_tag="O0293_002287"
     CDS             2816..3157
                     /gene="mnhC1"
                     /locus_tag="O0293_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit C"
                     /protein_id="HCY0281158.1"
                     /translation="MEIIMIFVSGILTAISVYLVLSKSLIRIVMGTTLLTHAANLFLI
                     TMGGLKHGTVPIYEANVKSYVDPIPQALILTAIVIAFATTAFFLVLAFRTYKELGTDN
                     VESMKGVPEDD"
     gene            3150..4646
                     /gene="mnhD1"
                     /locus_tag="O0293_002288"
     CDS             3150..4646
                     /gene="mnhD1"
                     /locus_tag="O0293_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit D"
                     /protein_id="HCY0281159.1"
                     /translation="MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLV
                     AAIINLANVVKHGPIRVELGSWKAPYSIVFVLDIFSALLIITSIIITAIVILYSYQTI
                     GIERERYYYYFSVLFMLIGIIGAFTTGDIFNLFVYFEVFLMSSYFLLVIGSTKIQLQE
                     TIKYVLVNVVSSSFFVMGVAILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIF
                     VFATKAGVFPMFVWLPSAYYAPPIPIIAFFGALLTKVGVYAIARTLSLFFSDNVSFSH
                     YVILFLALLTIIFGCVGAVAYANIKKIILYNVMIAVGVILVGVAMMTESGMIGAIYYT
                     LHDMLVKLALFLLIGIMIKITGTADLRQFGGLIKRYPVLGWSFFIAALSLAGIPPLSG
                     FYGKFFIVQSTFERGFYLSGVIVLLSSLVVLYSVIRIFLQGFFGQPKGYDLNNKVDVK
                     YLTTIAIVAVVITVLYGLSADYLYPMVKAGAETFYNPSTYVKAVLGGK"
     gene            4648..5127
                     /gene="mnhE1"
                     /locus_tag="O0293_002289"
     CDS             4648..5127
                     /gene="mnhE1"
                     /locus_tag="O0293_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit E"
                     /protein_id="HCY0281160.1"
                     /translation="MAVQLVLNFIIAVFWLFVTNSYTTNNFVLGFIFGLVLVYLLHRV
                     LPGRFYVITLYRIIKLVIIFLIELIKANFDVLKIIIKPSIKNEPGFFVYHTDLKKDWQ
                     IVLLSNLITLTPGTVVLGVSDDRTKIYIHAIDFSTKEQEVESIKTSLEKIVREVGEI"
     gene            5127..5420
                     /gene="mnhF1"
                     /locus_tag="O0293_002290"
     CDS             5127..5420
                     /gene="mnhF1"
                     /locus_tag="O0293_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit F"
                     /protein_id="HCY0281161.1"
                     /translation="MNHNVIIVIALIIVVISMLAMLIRVVLGPSLADRVVALDAIGLQ
                     LMAVIALFSILLNIKYMIVVIMMIGILAFLGTAVFSKFMDKGKVIEHDQNHTD"
     gene            5398..5754
                     /gene="mnhG1"
                     /locus_tag="O0293_002291"
     CDS             5398..5754
                     /gene="mnhG1"
                     /locus_tag="O0293_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit G"
                     /protein_id="HCY0281162.1"
                     /translation="MIKIILISLALIFVIIGALISALAAIGLLRLEDVYSRAHAAGKA
                     STLGAMSLLFGTFLYFIATQGFVNMQLIVAIIFVLITGPLSSHMIMKAAYNIKTPYTK
                     KTKVDEISEDLKDTKL"
BASE COUNT         1762 a          936 c         1001 g         2159 t
ORIGIN      
        1 acatattgcg gtgatattac cgttaatatt cgcactcatt attcctatcc tgtatagatt
       61 ttttaaacgt atacatttag gatggttcgt attacctgtc cctatcgtca tatttattta
      121 tatgcttaca ttaattaaaa cgacgatgtc aggtaatact gtaatgaaaa ccttaaattg
      181 gatgcctcat tttggtatga actttgattt atatcttgat ggcttaggtt tactctttag
      241 tttgcttatt tcaggaatcg gcagtttagt agtattatat tcaatcggct acttaagtaa
      301 gtcagaacaa ctagggaatt tttattgtta tttattacta tttatgggtg caatgttagg
      361 tgtcgtactt tcagataatg ttattattct atatttattc tgggagctaa cgtcattttc
      421 tagtttttta cttatttcat tctggcgcga acgacaagca tctatatatg gtgctcaaaa
      481 atcgctcata atcacagtat ttggtggtct atcattatta ggtggtatca tcttactcgc
      541 tataccaaca caatcattta gcattcagta catgattcaa catgcttctg aaattcaaaa
      601 tagcccattc ttcatttttg cgatgatttt aatcatgatt ggtgctttta caaaatctgc
      661 tcagtttcca ttttatattt ggctaccaga tgcaatggaa gcacctacac cagtcagtgc
      721 ttatcttcat tcagcaacaa tggttaaagc agggttatat ttaatcgctc gaatgacacc
      781 aatcttcgca gcatcgcaag gttgggtatg gactgtcaca ctcgttggtt taattacatt
      841 attctgggct tcgctaaacg caacaaaaca acaagacttg aaaggtatac tggcattttc
      901 aacagtatcc caattaggta tgattatggc gatgcttgga atcggtgcca ttagttatca
      961 ctatcaaggc gatgatagca aaatatatgc tgctgcattt acggctgcaa tattccattt
     1021 aatcaaccat gcaacattta aaggtgccct atttatgatt acaggtgcag tggatcattc
     1081 tactggtaca cgtgatgtta agaaattagg tggcttacta acaattatgc ctatttcatt
     1141 tacaatcact gttattacag cattaagtat ggctggtgta ccaccgttca atggtttctt
     1201 atcaaaagaa tcatttttag aaacaacatt tacagcaagc caagcaaatt tattcagtgt
     1261 tgatacatta ggctacctat tcccaatcat tggtatagtg ggcagtgtct tcacatttgt
     1321 atattcaatt aaatttataa tgcatatatt tttcggacaa tataaacctg aacaattacc
     1381 taaaaaagca cacgaagtat caatattaat gcttttatca ccagctattt tagctacatt
     1441 agtaattgta tttggtttat tccctggcat tttaactaat tcgattattg aaccagctac
     1501 atcttcgatt aatcatacgg taattgatga tgttgaattc catatgttcc atggattaac
     1561 gcctgccttt ttatcaacat tagtgattta tattttaggt atcttactaa ttgtgacatt
     1621 tagttattgg gtgaaattgt tgcaacgaca acctggtaaa ctaacattca attattggta
     1681 caacagaagt gcaaatgtta ttccaaacta ctctgaaaag atgacgaata gctatgttac
     1741 cgattattca cgtaataatt tagttatcat ttttggtgca ttaattttat taacatttgt
     1801 cactatattc tcagtacctt tcaacattaa ttttaaagac gttagtccga ttagaatttt
     1861 cgaagtttgt atcgtaattt tattattaag tgctgccttt ttaattcttt tcgctaaatc
     1921 aagattattt agcatcatta tgttaagtgc agttggatat gctgtgtctg tattgtttat
     1981 attctttaaa gcacccgact tagcattgac acagtttgtc gttgaatcta tttcaacagc
     2041 attgttctta ttatgcttct accacttacc aaatttaaat cgttataatg aaaagcgctc
     2101 attccagctt actaatgctt taattgctgg tggtgttggt ttgtccgtca ttattatcgg
     2161 tttaattgca tacggaaata gacattttga atcgatatct aagttttatc aagagcacgt
     2221 ttatgattta gctcacggta aaaacatggt aaacgtcata cttgttgatt tccgtggtat
     2281 ggatacttta ttcgagtcat ctgtgcttgg tatcgcagga ttagctgtat atacaatgat
     2341 taaattacgt aaaaagcgtc aaacgcaagg taacgaggtg aaaaatcatg aatagacaac
     2401 aaaatgatct aattttgcaa tttgctgcag ttattatctt tttcatggta atggtctttg
     2461 gtttttcact tttcttagca ggacattata cacctggtgg cggtttcgtc ggcggattat
     2521 tattcgcaag ttcacttgtt atcattacga ttgcatttga tatagaaaca atgcgtaaaa
     2581 ttttcccatt agattttaaa atcttgatag gtatcggatt agttttttgt attgcgacgc
     2641 ctatcgcaag ttggttttta ggcaaaaatt tctttacgca tgtcacattt gatataccgt
     2701 tatttatatt agaacctgtt catatgacaa cagcagtatt ctttgatttc ggtgttttat
     2761 gtgcagtagt tggtactgtc atgacaatta ttatttcgat tggagagaac gaatagtgga
     2821 aattattatg atttttgtta gtggtattct cacagcaatt agtgtctatc tcgttttgtc
     2881 taaaagtctg atacgaattg ttatgggaac tacactatta acacatgcag caaatttatt
     2941 tttaataact atgggcggac ttaaacatgg tactgttcca atttatgaag cgaacgtaaa
     3001 aagctatgtt gatcctatcc cgcaagcact tattttaaca gcaatcgtta tcgcctttgc
     3061 gacaacagcc tttttcttag tattagcatt tagaacatat aaagaattag gcacagataa
     3121 cgttgagagt atgaaaggag ttccagaaga tgattgaaag taatatgctt gtgttaacgc
     3181 tagttattcc agtaattaca gccatattac ttgtctttat tggaaagcgt cctattataa
     3241 agcgttatgt cgcactaggc ggtacattat taaccttggt cgcggcaatt atcaatttag
     3301 caaatgttgt taaacatgga ccaatacgcg tagagctagg ttcatggaaa gcaccataca
     3361 gtattgtctt tgtattagac atattcagtg ccctactaat aattactagt attattatta
     3421 cagctattgt gattttatat tcataccaaa caattggtat tgaacgtgaa agatattatt
     3481 attatttttc agtactattt atgttaatag gtatcattgg tgcattcaca actggtgaca
     3541 ttttcaacct attcgtatat ttcgaagtat ttttaatgtc atcttatttc ttattagtaa
     3601 ttggttctac aaaaattcaa ctacaagaaa caataaaata tgttcttgtt aacgttgtat
     3661 cgtcatcctt cttcgtaatg ggtgtagcga ttttatattc agtagtagga acattgaatc
     3721 tagcagatat aagcaataaa ttagctaacc tttcagcgca tgacagtggc ttagtcaata
     3781 tcgttttcat actatttatc tttgtttttg ctacaaaagc tggcgtattc ccaatgtttg
     3841 tttggttacc tagtgcatac tatgcaccac caattccaat cattgcattc tttggtgctt
     3901 tgttaacaaa agttggtgtt tatgcaatcg ctagaacatt gagcctattc tttagtgata
     3961 atgtaagctt ttcacattat gtcattttat tcttagcact attaacgatc atctttggtt
     4021 gtgtcggtgc tgttgcatat gccaacatta aaaaaatcat cctatacaat gtcatgattg
     4081 ctgtaggtgt gatcttagtc ggcgttgcaa tgatgacaga gtcaggtatg attggggcta
     4141 tttactatac attgcacgac atgttagtaa aacttgcatt attcttgctt atcggaatta
     4201 tgattaaaat tacaggcact gctgatttaa gacaatttgg tggcttaatt aaacgatatc
     4261 ctgtacttgg ttggtcattc tttatcgcag cattaagttt agctggcatt cccccactca
     4321 gtggctttta tggaaaattt tttattgtcc aatcaacatt tgaaagagga ttttatttaa
     4381 gtggtgtcat cgtcttatta tcaagtttag tcgtattata ttctgtcatt cgcatattcc
     4441 tacaaggctt tttcggtcaa ccaaaaggat atgatcttaa taacaaagtc gacgtcaaat
     4501 atttaactac gattgcaatc gttgcagttg tgataactgt tttatatgga ttgtcagcag
     4561 attacttata tccaatggta aaagctggtg cagaaacatt ctacaatcca tcaacctacg
     4621 ttaaagctgt attaggaggt aaataacatg gctgtacaat tagtattaaa ctttattatc
     4681 gcagtgtttt ggttgtttgt gacaaatagt tacacaacaa ataactttgt gttaggtttc
     4741 atctttggcc tagttcttgt ttacctctta cacagagtgt taccaggtag attttatgtc
     4801 atcacgctat atcgtattat taaattagtt attattttct taatcgaact gattaaagct
     4861 aattttgatg ttttgaaaat tattattaaa ccttcgatta aaaatgaacc aggattcttt
     4921 gtttatcaca cagatttaaa aaaagattgg caaattgtct tgctgtctaa tttaattaca
     4981 ttaacacctg gtactgtagt gcttggtgta agtgacgatc gtacaaaaat ttatattcat
     5041 gccatagatt tttcaactaa agagcaagaa gtagaaagta ttaaaacatc attagagaaa
     5101 attgtaagag aggtgggcga aatataatga atcataatgt tattatcgtt attgcattaa
     5161 tcatagttgt catttctatg ttagctatgc tcattcgcgt tgtgctaggc ccatcacttg
     5221 ccgatcgtgt tgtcgcatta gatgcgattg gtcttcaatt aatggcagtt atagcattat
     5281 tcagtatttt attaaatatt aaatacatga ttgtcgttat tatgatgatt ggtatattag
     5341 cttttttagg tactgcagta ttctctaaat ttatggacaa aggtaaggtg attgaacatg
     5401 atcaaaatca tactgattag tcttgcactt atctttgtta tcatcggtgc tttaattagc
     5461 gccctagcag ctataggatt attgagactt gaagatgtat attcacgtgc acatgctgcc
     5521 ggaaaagcat caacattagg tgcaatgtca ttactatttg gtacgttttt atattttatt
     5581 gctactcaag gttttgtaaa tatgcaatta atcgttgcga ttatctttgt attaattaca
     5641 ggtcctcttt caagtcatat gattatgaaa gcagcatata atattaaaac gccttatact
     5701 aaaaagacta aagtcgatga aatttcggaa gacttaaaag acacaaaatt gtagattata
     5761 taacaaaatc atgaatataa atcaacaaca aacagcagta agatgattcc aaattaggaa
     5821 tgattttact gctgttttct tttgacattg ttacctct
//
LOCUS       DAKTIO010000162         5835 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.164, whole genome shotgun sequence.
ACCESSION   DAKTIO010000162 DAKTIO010000000
VERSION     DAKTIO010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.164"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..628
                     /gene="sek"
                     /locus_tag="O0293_002292"
     CDS             <1..628
                     /gene="sek"
                     /locus_tag="O0293_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="staphylococcal enterotoxin type K"
                     /protein_id="HCY0281163.1"
                     /translation="FYTKKDFVDLKDVKDNDTPIANQLQFSNVSYDLISESKDFNKFS
                     NFKGKKLDVFGISYNGQCNNKYIYGGVTATNEYLDKPRNIPINIWINGNHKTISTNKV
                     STNKKFVTAQEIDVKLRKYLQEEYNIYGHNGTKKGEEYGHKSKFYSGFNIGKVTFHLN
                     NNDTFSYDLFYTGDDGLPKSFLKIYEDNKTVESEKFHLDVDISYKETI"
     gene            716..1936
                     /locus_tag="O0293_002293"
     CDS             716..1936
                     /locus_tag="O0293_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCY0281164.1"
                     /translation="MWLEKFKNKNKETKYRYYEKYKDPLTAKWRRVSVVLNKNGKQSQ
                     KEAQRLLNERIEEKLNDKKPTTLKSLTFHVACDEWFQNYIKTSGSKRTTIKTKLSKLN
                     TLKKFVDEDILINKITLSYAQQVFDEMDSKGYVYQVNKDALSIFKNVFEYTRRIYKLQ
                     DLEFLKDITLNKRIKSYDEVKAKRNKYLELNEIESIIKDINTKAQKMHSGIHKRFYLF
                     VALMTEFQALNGMRIGEMLAIQNEDIDFDNKSLNINGTIHWFHDESGGFGVKDTTKTE
                     SSYRTIGLSSRSCEILKKAILENKKDSKWNDGYLNRNFVFTNHKGNPMQTERFNKILR
                     EAAKDVGIDKEVSSHILRHSHISLLSQQGVSLKAIMDRVGHSDHRTTLSIYSHVTEQM
                     DKDMMNKLEQVKLG"
     gene            complement(2079..2900)
                     /locus_tag="O0293_002294"
     CDS             complement(2079..2900)
                     /locus_tag="O0293_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCY0281165.1"
                     /translation="MKKLFGLILVLTFAVVLAACGNGNKSGSDDKKITVGASPAPHAE
                     ILEKAKPLLEKKGYELDIKTINDYTTPNKLLDKGEIDANYFQHTPYLNTEKKDKGYKI
                     VSAGDVHLEPMAVYSKKYKSLKELPKGATVYVSNNPAEQGRFLKFFVDAGLIKIKKGV
                     KIEDAKFSDITENKKDIKFNNKQSAEFLPKIYQNEDADAVIINSNFAIEQKLNPKKDS
                     IAVESAKDNPYANLIAVKEGHQDDKKIKALIEVLQSKDIQDFINEKYNGAVILAK"
     gene            complement(2918..3613)
                     /locus_tag="O0293_002295"
     CDS             complement(2918..3613)
                     /locus_tag="O0293_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0281166.1"
                     /translation="MGKSFSEIINEMITMPNIQWPEVWTAIVETLYMTVVSTIFAFIL
                     GLILGVLLFLSAKGKSIGARLFYSIVSFIVNLFRAIPFIILILLLIPFTSLILGTISG
                     PTGALPALIIGAAPFYARLVEIAFKEIDKGVIEAAWSMGANTWTVIRKVLLPEAMPAL
                     VSGITVTAIALVGSTAVAGVIGAGGLGNLAYLTGFTRNQNDVILVSTVFILIIVFIIQ
                     FIGDWLTNKLDKR"
     gene            complement(3606..4631)
                     /locus_tag="O0293_002296"
     CDS             complement(3606..4631)
                     /locus_tag="O0293_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HCY0281167.1"
                     /translation="MIELKEVVKEYRTKNKEVLAVDHVNLSIRAGSIYGVIGFSGAGK
                     STLIRMFNHLEAPTSGEVIIDGDHIGQLSKNGLRAKRQKVSMIFQHFNLLWSRTVLKN
                     IMFPLEIAGVPRRRAKQKALELVELVGLKGREKAYPSELSGGQKQRVGIARALANDPT
                     VLLCDEATSALDPQTTDEILDLLLKIREQQNLTIVLITHEMHVIRRICDEVAVMESGK
                     VIEQGPVTQVFENPQHTVTKRFVKDDLNDDFETSLTELEPLEKDAYIVRLVFAGSTTT
                     EPIVSSLSTAYDIKINILEANIKNTKNGTVGFLVLHIPYISSVDFGKFEKELIERQVK
                     MEVLRHG"
     regulatory      complement(4720..4823)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4881..5177)
                     /locus_tag="O0293_002297"
     CDS             complement(4881..5177)
                     /locus_tag="O0293_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCY0281168.1"
                     /translation="MNNSLDIKDVTTFYEEDKHLIFGYTPTCGTCKVSERMLDIANEI
                     LQLPLLKIDLNFYPQFCKDMQIMSTPILLLMNKDKEVKRIYAFKSVTDLLENLK"
     gene            complement(5170..5556)
                     /locus_tag="O0293_002298"
     CDS             complement(5170..5556)
                     /locus_tag="O0293_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCY0281169.1"
                     /translation="MAIVNKVIIVEGKSDKKRVQQVIAEPVNIICTHGTMSIDKLDDM
                     IESLYDKQVFVLADSDDEGDRIRNWFKRYLSESEHIFIDKTYCQVANCPKQYLAHVLS
                     KHGFTCKKETPLLPNINNERLVLVNE"
BASE COUNT         2029 a          993 c          848 g         1965 t
ORIGIN      
        1 tttttataca aaaaaagact ttgtagattt aaaagatgta aaagacaatg atactcctat
       61 agctaatcaa ctacaatttt caaatgtatc ttatgattta atttcagaat caaaagattt
      121 taataaattt agtaatttca agggaaaaaa acttgatgtt tttggtatta gttataatgg
      181 ccagtgtaac aataaataca tatatggtgg agtcacagct actaacgaat atctagataa
      241 acctagaaat atccctataa atatatggat caatggaaat cacaaaacta tttctaccaa
      301 taaagtttcg acaaataaaa aatttgttac cgctcaagag attgatgtca aattaagaaa
      361 gtaccttcaa gaagaataca acatttatgg acataacggc actaaaaaag gagaagaata
      421 tggtcataaa tcaaaatttt attctgggtt taatattggt aaagtaacgt tccatttaaa
      481 taataatgac actttttcat atgatttatt ctacacagga gatgatgggt taccaaaaag
      541 ttttttaaaa atttacgaag acaataaaac tgtagagtct gagaaattcc atttggatgt
      601 cgatatttct tataaagaaa cgatataaat ctattaatta tgtcataatc attattttcc
      661 gggtagcatg cctaccctta ttattttttg ccaattttga ggagggagaa gcaaaatgtg
      721 gttagaaaaa tttaaaaata agaataaaga aactaaatat agatattacg agaagtacaa
      781 agaccctctc actgccaaat ggagacgtgt tagcgtggta cttaataaga atggtaagca
      841 atcacaaaaa gaagctcaaa gactcttaaa tgagcgtata gaagaaaaac tgaatgataa
      901 gaaacctact acacttaagt cactaacttt ccatgtcgca tgtgatgagt ggtttcagaa
      961 ttatatcaaa acgtctgggt caaaaagaac aactattaaa actaaattga gtaagctaaa
     1021 cactttgaag aaatttgtag atgaagatat tttaatcaat aaaataacac tttcatatgc
     1081 gcaacaagtt ttcgatgaaa tggatagtaa aggttatgta tatcaagtaa acaaagatgc
     1141 gttaagcata ttcaaaaatg tatttgaata cactagacgt atctataaac tgcaagattt
     1201 agaattttta aaagatataa cattaaataa aagaattaag tcttatgatg aagtgaaagc
     1261 taaacgtaat aagtacctcg aattaaatga aatagaatct atcatcaaag atattaatac
     1321 gaaggctcag aagatgcact caggtatcca taagcggttt tatttattcg ttgcactcat
     1381 gacagaattc caagccttaa atggtatgcg tattggtgaa atgttagcca ttcaaaatga
     1441 agatatagac tttgataata agagtttaaa tattaacgga acaatccatt ggtttcacga
     1501 tgaatctggt ggattcggtg taaaagatac cactaaaaca gagtctagtt atagaacaat
     1561 tggattgagt agcagaagtt gcgagatatt aaagaaagca atactggaga acaaaaagga
     1621 ttcaaaatgg aatgatggat atctaaatag aaattttgta tttactaatc ataaaggcaa
     1681 cccaatgcag actgaaagat tcaataaaat ccttagggaa gcagctaaag atgttggtat
     1741 tgataaagaa gtttcttcac atatattaag acatagccac atatcattac tctctcaaca
     1801 aggcgtgtca cttaaagcta ttatggatcg tgtaggccat tccgaccacc gtacaacact
     1861 ttccatttat tctcacgtta ctgaacagat ggataaggat atgatgaaca aacttgaaca
     1921 ggtaaaactt ggataatgcg ctcaaatctg agcgtatttt tttgtttaaa attcgataat
     1981 ttgcatatac tgcccctttt gtgccctttt ttattttcaa taccacaaaa ataacccctt
     2041 aatcgttgtt attaaagggt atttttattt aattggtatt atttagcaag aataactgca
     2101 ccattgtatt tttcattaat gaagtcttga atatctttag attgtaatac ttcaattaat
     2161 gctttgattt tcttatcatc ttgatgtcct tctttaacag caattaagtt tgcataagga
     2221 ttatctttcg cactttctac agcaatagaa tcttttttag gatttagttt ttgttcgatt
     2281 gcaaagttcg aattaatgat aacagcatca gcgtcttcat tttgataaat tttaggtaag
     2341 aattctgctg attgtttatt attaaactta atatcttttt tattctctgt aatatcacta
     2401 aacttagcat cttcaatttt tacgcctttt ttgattttaa ttaaacctgc atcaacgaag
     2461 aattttaaga aacgtccttg ttcagctgga ttattagaca catagactgt tgcacctttt
     2521 ggtaattctt ttaaactttt atacttttta gagtatacag ccataggttc taagtgaaca
     2581 tcaccggcac ttacgatttt gtaaccttta tcctttttct ctgtgtttaa atatggtgta
     2641 tgttggaaat agtttgcgtc aatttcacct ttgtctagta atttattagg tgtagtgtaa
     2701 tcgttaattg ttttaatatc tagttcataa ccttttttct ctaataatgg ttttgctttt
     2761 tctaaaattt cagcatgtgg tgctggtgaa gcacctactg ttattttctt gtcgtcactg
     2821 ccacttttgt ttccattacc gcaagctgct aatacaactg caaatgttaa tactaaaata
     2881 agaccaaata attttttcat aaaatgaaac ccccaattta tcgtttatca agtttatttg
     2941 taagccaatc cccaatgaat tggattataa atacaataat taaaataaaa actgttgata
     3001 ctaaaatgac atcattttga tttcgagtga aacctgttaa gtatgctaaa tttcctaaac
     3061 caccggcacc aattacacct gcaactgctg ttgaaccaac taaagcgatt gctgtaactg
     3121 taatgccaga cactagcgct ggcatagctt caggtaaaag gactttacga attactgtcc
     3181 aagtattagc gcccattgac caagccgctt cgatgacacc tttatcaatt tctttaaaag
     3241 caatttctac gagccttgca taaaacggtg ctgcgccaat gatcaaggct ggtaacgcac
     3301 ctgtcggacc acttatcgtt ccaagtatca aacttgtaaa tggaattaat aataaaatta
     3361 aaataataaa tggtatcgct ctaaataagt taacaatgaa agaaacgata gaataaaata
     3421 accttgcacc gatagactta cctttagcag acaagaataa taacacacct aaaataagac
     3481 caagtataaa tgcaaatata gttgagacga ctgtcatgta tagtgtttcg actattgcag
     3541 tccaaacttc tggccactga atattaggca ttgtaatcat ttcatttata atttcactaa
     3601 atgatttacc catgtcttaa cacctccatt ttaacttgtc gctcaattaa ctctttttcg
     3661 aattttccga aatctacact tgaaatatat ggaatatgca gaactaaaaa gccgactgtt
     3721 ccattttttg tatttttaat atttgcttct aaaatattaa ttttaatatc ataggcagtt
     3781 gatagactcg atacaatagg ctcggttgtt gttgaaccag cgaaaactaa tctaacgata
     3841 tatgcatctt tttctaatgg ctctaattct gttaaagatg tttcgaaatc atcatttaaa
     3901 tcgtctttca caaatcgttt tgtcacagtg tgttgcggat tttcaaaaac ctgtgtcacc
     3961 ggtccttgtt ctatcacttt accactttcc ataactgcaa cttcatcaca aatacgacga
     4021 atgacatgca tttcatgcgt aattagtaca attgttaaat tttgttgttc tctaattttt
     4081 agtagtagat ctaaaatttc atctgttgtt tgcggatcaa gtgcacttgt tgcctcatca
     4141 caaagcaaga ccgttggatc attagctaac gctcgtgcaa tcccaacacg ttgcttttgt
     4201 ccacctgata actctgatgg ataagccttt tctctacctt ttaaaccgac gagttcgaca
     4261 agttctaatg ctttttgctt agctctcctt ctagggacac ctgcaatttc aagcggaaac
     4321 ataatatttt ttaacacagt ccttgaccat aacaaattaa aatgttggaa gatcatactt
     4381 actttttgtc tttttgctct taatccattt ttggacaatt gacctatatg gtctccatct
     4441 ataataactt cacctgatgt aggcgcttct aaatgattaa acattcgaat caaagtactt
     4501 tttcctgctc cagaaaaacc aatgacgcca taaatcgatc ctgctcgaat cgataaatta
     4561 acgtgatcta cagcaaggac ttctttattt ttagtccgat attctttaac aacttctttt
     4621 aattcaatca cgttgattcc tccctgtgtt gcttaataaa ataaaaaatg ctttctcaat
     4681 atcgatagaa aaattgagaa agcaatagta gtattgtttc tctcatcttc aaaagttaaa
     4741 actttatgtg aattggcacc atttctatat aagacggttg ccgggcttcg tagggcacat
     4801 ccctccacca ctctcgataa gagtttacgc atcatttaat ttgtattaat cctaacacct
     4861 tagtaaaatt tcgtcaataa ctattttaaa ttttctaaca aatcagtcac cgatttaaat
     4921 gcataaattc gttttacttc tttatcttta ttcatcaaca ataaaatcgg cgtagacatg
     4981 atttgcatat ctttacaaaa ctgaggataa aagtttaaat ctattttcaa taatggtaac
     5041 tgcaatattt cattagcaat gtctaacatt ctttctgaaa ccttacaagt accacacgtt
     5101 ggtgtataac caaagattaa atgtttgtct tcctcataaa atgtagttac atctttgatg
     5161 tctaatgaat tattcattta ctaaaactaa cctttcatta tttatattcg gtaaaagagg
     5221 tgtttctttc ttacaagtaa agccatgttt tgaaagtaca tgcgccaaat attgtttggg
     5281 gcaattcgca acttgacagt aagttttatc aataaatata tgttcacttt cactcaaata
     5341 acgtttaaac caatttctaa ttcgatctcc ttcgtcatca gaatcggcta atacaaaaac
     5401 ttgtttatca tacagtgatt ctatcatatc atcaagctta tctatactca ttgttccatg
     5461 agtacaaata atattgactg gttctgcaat aacctgttgc accctttttt tatcagattt
     5521 tccttcaaca attatcactt tatttacaat agccatcatc atcacccttt aaaatcaata
     5581 aacatctgtc actgtatcat ttcacaaaat tggtatgaat aaaacataaa tcacaaaaaa
     5641 tttaaactag cttaatataa taattacaaa ctcaatgttt gactagctgg aacatttaac
     5701 ataagcagac aaaggctaag tcaaaaatca acatcctaaa atctacaatg ttatattaac
     5761 aatagttaac caaaagaaaa tacacctata acaaactttt caattatagc ggggccccaa
     5821 cacagaagct gatgg
//
LOCUS       DAKTIO010000163         5614 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.165, whole genome shotgun sequence.
ACCESSION   DAKTIO010000163 DAKTIO010000000
VERSION     DAKTIO010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.165"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            139..1089
                     /locus_tag="O0293_002299"
     CDS             139..1089
                     /locus_tag="O0293_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCY0281170.1"
                     /translation="MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTRIIAEHDY
                     QPNQFAQSLRARQTHLIGAIIPRMNSYAVDETIKGLAKQCQKYESQLILNYTGLNIEA
                     EIQALETLARSKVDGIVLMATDITERHIEVINKMNVPIVIVGQQHEQLHSIVHDDYKA
                     GQIIGEWIGQQGYQQVEVFSVSEKDIAVGIHRKRGLLDQLAKYQIKPNIHETNFTYVE
                     AQKDVANVLENVEQVDAVVGATDTIALAAYKYYSDKKDVMKPHQIYGFGGDPMTQLVS
                     PSIKTIHYNYFEAGQCAMEEIQQMLKKQDMPYSVTVDVNI"
     gene            1238..2722
                     /locus_tag="O0293_002300"
     CDS             1238..2722
                     /locus_tag="O0293_002300"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCY0281171.1"
                     /translation="MTEWTREERYQRIEDVDTEYFKTLKQQVDQSKFRQQFHIQPETG
                     LLNDPNGLIFYKGKYYVSHQWFPLGAVHGLKYWYNYTSDDLINFKAEGPILNPDTKYD
                     SHGVYSGSAFEYNGHLYYMYTGNHRDNHWQRHASQMIARLKEDGSVEKFPKPVISQQP
                     EGYTSHFRDPKVFKYDEKYYAIIGAQNNDQQGRLLLYNTEDIINWHYLGEINTELDDF
                     GYMWECPDYFNVDNQDVILICPQGIEPKGDQFKNIYQSGYILGKFDIEKLTYEHENFV
                     ELDNGFDFYAPQTFLDEKGRRVLIGWMGLPEIEYPTDNEGWAHCLTIPRVLNVENGQL
                     KQRPYPALEKLRHNKETALGYANKFTRKLHPYEGKQYELIIDILDNDATEVYFELRTS
                     KTSSTLIAYNKRENKITLDRSDSGLLPTNVEGTTRSTILDTPLKQLQIFVDTSSIEIF
                     CNDGERVLTSRIFPTEDALGIKTSTESGQVYLQFTKYDLKDEHK"
     gene            2719..3678
                     /locus_tag="O0293_002301"
     CDS             2719..3678
                     /locus_tag="O0293_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCY0281172.1"
                     /translation="MRRLFSIGEALIDFIPNVTNANLKDVQTFTKQIGGAPCNVACTV
                     QKLGQQAYMITQLGNDAFGDSIIETISSIGVDVSKVYRTNEANTALAFVSLTEAGERD
                     FSFYRKPSADMLFEPSFVNDIDVNENDVVHFCSVDLVDSPMRDAHYQLITKTLNANGT
                     VVFDPNVRLPLWDNAEDLRQTIHTFLPLAHIVKVSDEELEFITGIHDENEAIQSLFTG
                     NVTVVIYTKGADGAAVYLKNGINHYHSGYKVKPVDTTGAGDAFIGAVISRILATDVLN
                     LTQLFENEGEEILAFSNRVAAIVTTKYGAINSLPTLQEVEQAF"
     gene            complement(3866..3973)
                     /locus_tag="O0293_002302"
     CDS             complement(3866..3973)
                     /locus_tag="O0293_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281173.1"
                     /translation="MHCIAGFLILLTKLNMTRELKNDVTSLCIPIPITT"
     gene            complement(4045..4761)
                     /locus_tag="O0293_002303"
     CDS             complement(4045..4761)
                     /locus_tag="O0293_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0281174.1"
                     /translation="MKIFICEDDPKQRENMVTIIKNYIMIEEKPMEIALATDNPYEVL
                     EQAKNMNDIGCYFLDIQLSTDINGIKLGSEIRKHDPVGNIIFVTSHSELTYLTFVYKV
                     AAMDFIFKDDPAELRTRIIDCLETAHTRLQLLSKDNSVETIELKRGSNSVYVQYDDIM
                     FFESSTKSHRLIAHLDNRQIEFYGNLKELSQLDDRFFRCHNSFVVNRHNIESIDSKER
                     IVYFKNKEHCYASVRNVKKI"
     gene            complement(4780..>5614)
                     /locus_tag="O0293_002304"
     CDS             complement(4780..>5614)
                     /locus_tag="O0293_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCY0281175.1"
                     /translation="ISTILLLTFSLFFFYSQINSDEAKVIRQYSFIFIGITIFLSILT
                     FVISQFLLKEMKYKRNQEEIETYYEYTLKIEAINNEMRKFRHDYVNILTTLSEYIRED
                     DMPGLRDYFNKNIVPMKDNLQMNAIKLNGIENLKVREIKGLITAKILRAQEMNFPISI
                     EIPDEVSSINLNMIDLSRSIGIILDNAIEASTEIDDPIIRVAFIESENSVTFIVMNKC
                     ADDIPRIHELFQESFSTKGEGRGLGLSTLKEIADNADNVLLDTIIENGFFIQKVEIIN
                     N"
BASE COUNT         2014 a          796 c          994 g         1810 t
ORIGIN      
        1 ggttgatgtt gggacatttt gttatacaaa agttttattt tgaaatcttt ttatgaaaga
       61 agcagaaata ttatttaaag cggttacaca tatgctaaaa taaggctaag tgtcacaaat
      121 aatgataggt gaataagtat gaaaaatata tctgatattg ccaaattggc aggcgtttca
      181 aaaagtacag tatctagatt tttaaataat ggatctgtca gtaaaaaaac aagtgaaaaa
      241 ttaacaagaa ttatagcaga acatgactat caaccgaatc aatttgctca aagtttaaga
      301 gcgagacaaa cacaccttat tggtgccatt atacctagaa tgaattcata tgcagtagat
      361 gaaacaatca aaggattggc aaaacaatgc caaaaatatg aatcacaatt aattttaaat
      421 tacacaggtt taaatatcga agcagaaata caagcgcttg aaacattagc acgcagtaaa
      481 gtagatggta ttgttttaat ggctacagac ataacagaga gacatattga agtcattaat
      541 aaaatgaatg taccaatcgt tattgttggt caacaacatg aacaacttca tagtattgtg
      601 catgatgatt ataaagcagg tcaaattata ggcgaatgga ttggtcaaca gggatatcaa
      661 caagttgaag tgtttagtgt aagtgaaaaa gatattgcag ttggtataca tagaaaacgt
      721 ggtttacttg accagttagc taaataccaa attaaaccta atatacatga aacaaatttt
      781 acttatgtgg aagcacaaaa agatgttgca aatgttttgg aaaatgtgga gcaagtagat
      841 gcggttgttg gagcaactga tacgattgca ttagctgcct ataaatatta ttctgataaa
      901 aaagatgtta tgaaaccaca tcaaatatat ggttttggtg gtgacccaat gacacaatta
      961 gtgtctccat cgataaaaac aattcattat aattattttg aagctggcca atgcgcgatg
     1021 gaagagatac aacagatgct taaaaagcaa gatatgccat atagcgtcac agtagatgtt
     1081 aatatttaga cgctgtattt tttaaaataa atgtggaacc gataccatat aactataaat
     1141 ggataggtta aaagttaaag aacgtaggta aaatttgcta taatagaata taaattgtta
     1201 ttagcataaa ttataaaagg aggactgggt aaatattatg accgaatgga ctagagaaga
     1261 acgttatcaa cgaatcgagg acgttgatac tgagtatttt aaaacattaa aacaacaagt
     1321 tgatcaatca aaatttcgtc aacaatttca tatacaacca gaaacaggct tattaaatga
     1381 ccccaacgga cttatttttt ataaagggaa gtattatgtt tcacatcaat ggttcccatt
     1441 aggcgcagta catggcttaa agtattggta taactacacg agtgatgact taataaactt
     1501 taaagctgaa gggccaattt taaatccaga tactaaatat gacagccatg gtgtatatag
     1561 cggtagcgct tttgaatata acgggcattt atattatatg tacacaggaa atcatcgaga
     1621 taatcattgg caacgacatg cgagtcagat gatcgcacga ttgaaagaag acggttcagt
     1681 tgaaaagttt ccaaagccag taattagcca acaaccagaa ggatatacga gtcattttag
     1741 agatcctaaa gtttttaaat atgatgagaa atattatgca atcattggtg cacaaaataa
     1801 tgatcagcaa ggtcgattat tactttataa tactgaagat ataattaatt ggcattattt
     1861 aggtgaaata aatacagagt tggatgattt tggatatatg tgggaatgcc cagattactt
     1921 taatgtagat aatcaagatg tcatacttat ttgtccacaa ggtattgaac caaaaggcga
     1981 tcagttcaaa aatatttatc aaagtggtta tatacttgga aagtttgata ttgaaaagtt
     2041 aacatatgaa catgaaaatt ttgtcgagct tgataatggt tttgatttct atgcacctca
     2101 aacattttta gatgaaaaag gccgacgagt actaattgga tggatggggt taccggaaat
     2161 cgaatatcct actgataatg aaggatgggc ccattgctta acaattcctc gtgtattaaa
     2221 tgtagaaaat ggtcaactta agcaacgtcc atatccagcg ttggaaaaat tacgtcacaa
     2281 taaagagaca gcattaggct acgcaaataa atttactcga aaattacatc cgtatgaagg
     2341 taaacagtat gaattaatca tagatatttt ggataatgat gctaccgaag tgtactttga
     2401 attacgtaca tctaagactt cttcaacatt aattgcttat aacaagcgtg aaaataaaat
     2461 aacattagat cgcagcgaca gtggtttatt gccgacaaat gttgaaggta cgacgcgtag
     2521 tacgatatta gacacgccat taaagcaatt acaaattttt gttgatacat ctagtataga
     2581 aattttctgt aatgatggag aacgtgtttt gacatctcga attttcccga cagaagacgc
     2641 actaggtatt aaaacatcaa cagaatcagg gcaagtatat ttacaattta ctaaatatga
     2701 tttaaaggat gaacataaat gagacgtcta ttttcaatag gagaagcgtt aattgatttt
     2761 attccaaatg ttacaaatgc gaatttaaaa gatgttcaaa catttacgaa gcaaattggt
     2821 ggcgcgccat gcaatgtagc ttgtacagtt caaaagttag gacaacaagc gtatatgatt
     2881 acacaattag gtaatgatgc atttggagat agcattattg aaacgatatc aagtattggt
     2941 gtagatgtat caaaagtgta tcgcacgaat gaagcaaaca ctgcgttagc ttttgtgagt
     3001 ttgactgaag cgggcgagcg agatttttct ttttatcgta agccctctgc tgatatgtta
     3061 tttgaaccaa gttttgtgaa tgatattgat gtgaatgaaa atgatgtagt acatttttgt
     3121 tctgttgatt tggtcgatag tccgatgaga gatgcacatt atcaattgat taccaaaaca
     3181 ttaaatgcaa atggtactgt cgtatttgat cctaatgtaa gattgccatt atgggataac
     3241 gctgaagatt taagacaaac tattcatacc tttttgccat tggcgcatat tgtaaaagtt
     3301 tcagatgaag aattagaatt tattacaggt attcatgatg aaaatgaagc cattcaatca
     3361 ttatttacag gtaatgttac tgtagtcatt tatacgaagg gagcagatgg tgctgccgta
     3421 tatttgaaaa atggtattaa tcattatcat agtggctata aggtaaaacc tgttgataca
     3481 actggggcag gggatgcatt tattggtgca gttattagca ggattttagc aaccgatgtg
     3541 ttaaatttaa ctcagttatt tgaaaatgaa ggtgaggaaa ttttagcgtt tagcaatcgc
     3601 gttgctgcaa tagtgacaac gaagtatggt gctattaata gtttgccaac attacaagag
     3661 gttgaacaag cattttaaat aattcattta taaaacagtg taggaaacaa gttgaatctt
     3721 gtgctacact gttttttaaa acaaaataag attcacggag taggaaatta tagcatttta
     3781 tttatggaaa ctgtcaattt caaatttagt atattttgtt aaaaattgcg ccataggatt
     3841 gtagagtgaa atagaaattg ataaattatg tagtaatagg aataggtata caaagagacg
     3901 ttacatcatt ttttaattca cgcgtcatat ttaattttgt taataaaatt aaaaagccag
     3961 ctatacagtg catttgctag ttatcttgtt aaaatccaac aagatttaca attgaatacg
     4021 ccgttaactg actttattat cttattatat ttttttaacg tttctcaccg atgcatagca
     4081 gtgttcttta tttttaaaat agacaattcg ctctttcgaa tctatagatt caatattatg
     4141 gcgattgacg acaaagctat tatgacatct aaagaaacga tcatctaatt gactcagttc
     4201 ttttaaatta ccataaaatt caatttgacg gttatctaaa tgggcaatga gtctgtgaga
     4261 ttttgttgat gattcaaaaa acataatatc atcatattga acatacactg aattactgcc
     4321 acgttttaat tcaatcgttt caacgctatt atctttagac aacaattgta agcgtgtatg
     4381 tgcagtttct aaacagtcta taattcgagt tcttaattca gctggatcat ctttaaaaat
     4441 aaaatccatc gctgcaactt tgtagacaaa tgttaaatag gtaagttcac tgtgactcgt
     4501 aacgaaaata atgttaccaa ctgggtcatg cttacgaatt tcactgccta atttgatacc
     4561 attaatatca gttgaaagtt gaatatctaa aaagtaacag cctatgtcat tcatattttt
     4621 agcttgctca agcacctcat aaggattatc agttgcgagg gcaatttcca taggcttttc
     4681 ttctatcatt atataatttt taataatggt aaccatgttt tctctttgtt ttggatcgtc
     4741 ttcgcaaatg aaaattttca tacattcaca tccttatggc tagttgttaa taatttcaac
     4801 tttttgaata aagaaaccat tttcgataat tgtatctaat aagacattgt ctgcattatc
     4861 agcaatttct tttaaagttg atagacctaa accacgacct tcacctttag tagaaaaact
     4921 ttcttggaac aattcatgaa tgcgtggtat atcatcagcg catttattca taacaataaa
     4981 cgttactgaa ttttcacttt caataaatgc aacgcgaatg atagggtcat caatttcagt
     5041 tgatgcctca attgcattat caagaataat accaatactg cgacttaaat cgatcatatt
     5101 caagttaatg ctacttactt catcgggtat ttcgatacta atcggaaaat tcatttcttg
     5161 tgcacgtaaa attttcgcag taattaagcc tttaatttca cgtactttaa gattctcgat
     5221 accatttaat tttatagcat tcatttgtaa attgtctttc ataggtacaa tatttttatt
     5281 gaaataatca cgtaggccag gcatgtcatc ttctcgaatg tattctgaaa gtgtcgttaa
     5341 gatattgaca taatcatgac ggaacttgcg catttcgttg ttgatagctt caatcttcaa
     5401 tgtatattca taataggttt caatttcttc ttgattacgt ttatatttca tctctttaag
     5461 gagaaattga gaaataacaa atgttaatat acttaaaaat atagtgatac caataaaaat
     5521 aaaagaatac tgccttatta ctttagcttc atccgagttt atttgtgaat aaaagaaaaa
     5581 taatgaaaaa gtaagcagta agatagtcga aata
//
LOCUS       DAKTIO010000164         5591 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.166, whole genome shotgun sequence.
ACCESSION   DAKTIO010000164 DAKTIO010000000
VERSION     DAKTIO010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.166"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            321..1622
                     /locus_tag="O0293_002305"
     CDS             321..1622
                     /locus_tag="O0293_002305"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HCY0281176.1"
                     /translation="MRMIDIIEKKRDGHTLTTEEINFFIGGYVKGDIPDYQASSLAMA
                     IYFQDMNDDERAALTMAMVNSGDMIDLSDIKGVKVDKHSTGGVGDTTTLVLAPLVAAV
                     DVPVAKMSGRGLGHTGGTIDKLEAIDGFHVEIDEATFVKLVNENKVAVVGQSGNLTPA
                     DKKLYALRDVTGTVNSIPLIASSIMSKKIAAGADAIVLDVKTGSGAFMKTLEDAEALA
                     HAMVRIGNNVGRNTMAIISDMNQPLGRAIGNALELQEAIDTLKGQGPKDLTELVLTLG
                     SQMVVLANKAETLEEARALLIEAINSGAALEKFKTFIKNQGGDETVIDHPERLPQAQY
                     QIEYKAKKSGYVTELVSNDIGVASMMLGAGRLTKEDDIDLAVGIVLNKKIGDKVEEGE
                     SLLTIHSNRQDVDDVVKKLDSSITIADHVVSPTLIHKIITE"
     gene            1636..1986
                     /locus_tag="O0293_002306"
     CDS             1636..1986
                     /locus_tag="O0293_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281177.1"
                     /translation="MNILRYLLGIGFSVIGVLHFTRERQFRNIIPKCLPLRKTAVLVT
                     GIFEILFGLALLIKRPSQCLKNTINLFLLAVFPANIYMAVKKLPLGDKQLPTWQLYLR
                     LPMQFVLMGLIKKL"
     gene            complement(2138..2608)
                     /locus_tag="O0293_002307"
     CDS             complement(2138..2608)
                     /locus_tag="O0293_002307"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCY0281178.1"
                     /translation="MTKMNVESFNLDHTKVVAPFIRLAGTMEGLNGDVIHKYDIRFKQ
                     PNKEHMDMPGLHSLEHLMAENIRNHSDKVVDLSPMGCQTGFYVSFINHDNYDDVLNIV
                     EATLNDVLNATEVPACNEVQCGWAASHSLEGAKTIAQAFLDKRNEWHDVFGTGK"
     gene            2919..4103
                     /locus_tag="O0293_002308"
     CDS             2919..4103
                     /locus_tag="O0293_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCY0281179.1"
                     /translation="MSEKQQILDYIETNKYSYIEISHRIHERPELGNEEIFASRTLID
                     RLKEHDFEIETEIAGHATGFIATYDSGLDGPAIGFLAEYDALPGLGHACGHNIIGTAS
                     VLGAIGLKQVIDQIGGKVVVLGCPAEEGGENGSAKASYVKAGVIDQIDIALMIHPGNE
                     TYKTIDTLAVDVLDVKFYGKSAHASENADEALNALDAMISYFNGVAQLRQHIKKDQRV
                     HGVILDGGKAANIIPDYTHARFYTRAMTRKELDILTEKVNQIARGAAIQTGCDYEFGR
                     IQNGVNEFIKTPKLDDLFAKYAEEVGEAVIDDDFGYGSTDTGNVSHVVPTIHPHIKIG
                     SRNLVGHTHRFREAAASVHGDEALIKGAKIMALMGLELITNQDVYQDIIEEHAHLKGN
                     GK"
     gene            4103..5296
                     /locus_tag="O0293_002309"
     CDS             4103..5296
                     /locus_tag="O0293_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HCY0281180.1"
                     /translation="MTNNGNEPNLTLSDLYDKDVVYTSRPSYISNPWLKPDEHQSNFL
                     TGRELLIANQLPVIVHEASATDKLHQLFQVIGKEVPNSIYTFNNQQSYENLIKQLAHK
                     ENKKIYFQYIHDETILNQQYYALDKTLFVALNNKARIPEWTNGKFLPKRKVVKIEQFE
                     NEIKNWEFPLVIKPGDDLPTAGGYGVMICYHDADLQKAITRIKEATAETNSLIIEQKI
                     EEKANYCVQFAYSESLGIQYLGAATQLTDKYGFYNGNENTTNVPEHVIEAGRQIMENG
                     VNQGFFGVAGFDLLVDEDDNVYAIDLNFRQNGSTSMLLLANELNSGYQKFYSYHSKGD
                     NTHFFNTILKYVKEGSLYPLSYYDGDWYGEDKVKSRFGCIWHGDSKETVLENERAFLA
                     ELEHY"
BASE COUNT         1935 a          765 c         1129 g         1762 t
ORIGIN      
        1 gttcaaatta tgcaaggttt agaagcagat tcagattact aattttataa aaatatgtaa
       61 gcatgggaca tcgtaaccat atataagtat gtcgatatca gctttaagac atcataaatg
      121 ccatgatgat tctaagataa tttaataggg actttaacgt tataaccaat ctatttaaga
      181 ataataacat cgcattgaaa ttgtgatatt aaaaatgaaa tcgatagctt gctataataa
      241 ttttcgcttg tgcttttaaa aatggactag agaaagcgca gatgctttgt cctaagcttt
      301 ttaagaaaag gatgataaaa atgagaatga tagacattat tgagaaaaag cgtgacggtc
      361 atacactaac aacggaagaa attaatttct ttattggcgg ctatgttaaa ggggatattc
      421 ctgattacca agcatcaagt ttagcaatgg cgatttattt ccaagatatg aatgatgatg
      481 agcgtgcagc attaacgatg gctatggtta attctggtga tatgatagat ttgagtgata
      541 ttaaaggtgt caaagtagat aagcactcaa caggtggtgt aggagataca actacattgg
      601 ttttagcacc attagtagca gctgtagatg ttcctgttgc aaaaatgagt gggcgtggat
      661 taggtcatac aggtggtacg attgataaat tagaagcaat tgatggtttt catgttgaaa
      721 tagatgaagc aacatttgtg aaattggtta atgaaaataa agtggcagtt gtaggacaat
      781 caggaaattt aactcctgca gacaaaaaat tatatgcctt aagggatgtt actggtactg
      841 tcaattcaat accattaatt gcctcttcaa ttatgagtaa aaagattgct gctggtgcag
      901 atgcaattgt attagatgta aaaactggta gcggtgcatt tatgaaaaca ttagaagatg
      961 ctgaagcatt agcgcacgca atggtacgta ttggtaataa tgtgggacgt aatacgatgg
     1021 cgattatttc tgatatgaat cagccacttg gacgtgcgat tggcaatgcg cttgagttac
     1081 aagaagcaat tgatacgttg aaaggacaag gtccgaaaga tttaacagaa cttgtattaa
     1141 cattaggttc tcaaatggtt gtacttgcaa acaaagctga aacgcttgaa gaagcgagag
     1201 cgttgctaat tgaggcaatt aattcaggtg cagcattaga aaaattcaaa acatttatta
     1261 aaaaccaagg tggcgacgaa actgttattg accatccaga gcgtttgcca caagctcaat
     1321 atcaaattga atataaagct aaaaaatcag gttatgtgac tgaattagtc tctaacgata
     1381 taggtgtcgc ttcgatgatg ttaggagcgg gacgtttaac aaaagaggat gatattgatt
     1441 tagcggttgg tattgtttta aataaaaaaa ttggtgataa agtagaagaa ggagaatcat
     1501 tattaacgat tcacagtaat cgccaagatg tagacgatgt tgttaaaaaa ttagattcaa
     1561 gcataacaat tgctgatcat gttgtatcgc ctacattaat tcataaaatc attacagaat
     1621 aggagttgaa gctgattgaa catactaaga tatttattag gaataggttt tagtgtaata
     1681 ggtgtattgc attttacacg agaacgacaa tttagaaata tcataccgaa atgtttgcca
     1741 cttcgaaaaa cagcggttct tgttacagga atctttgaga tattatttgg acttgcactt
     1801 ttaattaaaa gaccatctca atgcttgaaa aatacaatca atctattctt gctagctgta
     1861 ttcccagcta atatctatat ggcagttaaa aagttacctt taggagataa acaattgcca
     1921 acatggcaac tatatttaag gttacctatg cagttcgtgt taatgggact aattaagaaa
     1981 ttatagtggt tctcaaataa atcggacaaa tgttaaagat tttacttttt catttgttcg
     2041 attttttgac tttgaagcat aataaaaagc agttggacaa tggcgattgt aaaatcattg
     2101 ttcgactgct tttctgatta acttgattga ctaagattta ttttcctgta ccgaaaacat
     2161 catgccattc gtttcgtttg tctagaaatg cttgagcgat agttttagca ccttctaatg
     2221 aatgacttgc tgcccagcca cattgtactt cattacaagc aggcacttca gtagcattta
     2281 gcacatcatt taaagttgct tcaacaatat ttaatacatc atcataatta tcatgattaa
     2341 taaatgatac atagaaacca gtttggcaac ccataggact taaatcaaca actttgtcac
     2401 tatgatttct aatattttca gccattaaat gttctaatga atgtagtccg ggcatatcca
     2461 tatgttcttt gtttggttgt ttgaaacgaa tgtcgtattt gtgaatgaca tctccgttta
     2521 atccttccat cgttcccgct aatctaataa atggggcaac caccttagta tgatctaaat
     2581 tgaaactttc aacattcatt tttgtcattt tgaatttcct cctattaact actcaaaact
     2641 ataaaacgct tatgatttgt tgcattataa aacttaagtt tgttgtaagt tgtgtttcta
     2701 acaatactta aattgtaaca atagggctta tgttttacaa tttgaaactg ttttataaat
     2761 ttatgaaagg cttttagtta ttagaaaatt ttgtaaataa agaaaaacta gatgacatca
     2821 tgagataaaa ccgatagaat aagtaataat tcattttgaa agaacaaaat attgtggatg
     2881 attaaaatga gtgtagggta aaagggagat agtaaaaaat gagtgaaaaa caacaaattc
     2941 tcgattatat agaaacaaat aaatatagtt atatcgaaat cagtcataga attcatgaac
     3001 gtcctgaact tggtaatgaa gaaatatttg cgtctcgaac tttaatagat cgtttgaaag
     3061 agcatgattt tgaaattgaa accgagattg ctgggcatgc aactgggttt atagcgacat
     3121 atgattcagg acttgacggg ccagctatag gttttttagc agaatacgat gctttgccag
     3181 gattgggtca tgcttgtggt cataatatca ttggaactgc aagtgttctt ggtgcaattg
     3241 gtttgaagca agtgattgac caaattggtg gtaaagtagt cgttcttgga tgtccagctg
     3301 aagaaggtgg ggaaaatggt agcgctaaag cttcttatgt caaggctggt gtgattgatc
     3361 aaatagacat tgccttaatg attcatccgg gaaatgaaac ttataaaacg attgatactt
     3421 tggcagtgga tgttttagat gttaaatttt acggaaaaag tgctcatgcc tctgaaaatg
     3481 cagatgaagc gttaaatgca ttagacgcta tgattagtta ttttaatggt gtagcacaac
     3541 tacgacaaca tattaaaaaa gatcaacgtg tgcatggtgt gattttagat ggcgggaaag
     3601 cagctaatat tattccagac tatacacatg ctcgttttta tactagagca atgacgcgta
     3661 aagaattgga tatattaaca gaaaaagtaa atcaaatcgc acgtggagct gcgatacaga
     3721 ctggttgtga ttatgaattt ggtcgaattc aaaacggtgt gaatgaattc attaaaacgc
     3781 cgaaattaga tgatttattt gctaaatatg ctgaagaagt tggtgaagca gttattgatg
     3841 atgattttgg ttatggctct acggatacag ggaacgtaag tcatgttgtg ccaacaatac
     3901 atcctcatat taaaatagga tcacgtaatt tagtaggaca tacgcataga tttagagaag
     3961 cggctgcgag tgtacatggt gatgaagcat taattaaagg cgctaaaata atggcgttga
     4021 tggggttaga gttaattaca aatcaagacg tttatcaaga cattattgaa gagcatgcgc
     4081 atttgaaagg gaatgggaag taatgacaaa taatggtaat gagccgaact taacgttaag
     4141 tgatttatat gacaaagatg ttgtctatac gtcaagacct tcgtatatat cgaatccatg
     4201 gttgaaaccg gacgaacatc aatctaattt tttaactggt agggagttat taatagcaaa
     4261 tcaattacct gttattgttc acgaagctag tgcgacagac aaattgcatc agctatttca
     4321 agttattggt aaggaagtac cgaactcaat ttatacattt aacaatcagc aatcttacga
     4381 aaacttgatc aaacaattgg cacataagga aaataaaaag atttattttc aatatattca
     4441 tgatgaaaca atcttgaatc agcaatatta tgcattagat aaaacgttat ttgttgcttt
     4501 aaataataaa gcaagaatac ctgaatggac aaatgggaag tttttaccaa aaagaaaagt
     4561 tgtaaaaatt gagcaattcg aaaacgagat taagaattgg gaattcccgt tagtaattaa
     4621 gccgggagat gatcttccga cagcaggtgg ctatggcgtt atgatttgtt atcatgatgc
     4681 agatttacaa aaagcgataa cgcgtattaa agaggcaact gctgaaacga attcgcttat
     4741 tatcgaacag aagattgaag aaaaagcgaa ttattgcgtt cagtttgcat actcggaatc
     4801 gctaggcatt caatatcttg gtgcggcaac gcaattaacg gataagtacg gattttataa
     4861 cggaaatgaa aatactacta atgtaccaga gcacgttata gaagcaggta gacaaattat
     4921 ggaaaacggt gtgaatcaag gattctttgg tgtagctggt tttgacctac tcgtcgatga
     4981 ggatgataac gtttatgcga ttgatttaaa ctttagacaa aatggttcaa cgagcatgtt
     5041 attacttgct aacgagttga attcaggata tcaaaagttt tatagttatc attcaaaagg
     5101 tgataacaca catttcttca atacgatttt gaaatatgtc aaagaaggta gtttataccc
     5161 gttatcttat tatgatggtg attggtacgg tgaagataaa gttaaatcaa ggtttggctg
     5221 tatttggcat ggtgattcaa aagaaacagt actggagaat gaacgcgcat ttttagctga
     5281 acttgaacac tattagagtt cggaacataa ggcgctacaa tgttgtgttg ccagtagttg
     5341 actgaatatg cgtttgtaac aagctttttt cgattctagt caacagtaat taaatttatg
     5401 atatggcaat actttgtaat actaatatta aatggcgact tttatttcac tatgttataa
     5461 gagttgccat tttgttgata aaggtatact aaaggttatc gttttgaaat ttttagtaac
     5521 tagatatgtt tcgtgttata gaccgaattt gtgtatacgt aaaatttaat gctattgaat
     5581 ttttaaaatg a
//
LOCUS       DAKTIO010000165         5464 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.167, whole genome shotgun sequence.
ACCESSION   DAKTIO010000165 DAKTIO010000000
VERSION     DAKTIO010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5464
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.167"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(54..1664)
                     /locus_tag="O0293_002310"
     CDS             complement(54..1664)
                     /locus_tag="O0293_002310"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HCY0281181.1"
                     /translation="MTKFIFVTGGVVSSLGKGITASSLGRLLKDRGLNVTIQKFDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKFSNVTAGKVYSHVLKKE
                     RRGDYLGGTVQVIPHITNEIKERLLLAGESTNADVVITEIGGTTGDIESLPFIEAIRQ
                     IRSDLGRENVMYVHCTLLPYIKAAGEMKTKPTQHSVKELRGLGIQPDLIVVRTEYEMT
                     QDLKDKIALFCDINKESVIECRDADSLYEIPLQLSQQNMDDIVIKRLQLNAKYETQLD
                     EWKQLLDIVNNLDGKITIGLVGKYVSLQDAYLSVVESLKHAGYPFAKDIDIRWIDSSE
                     VTDENAAEYLADVDGILVPGGFGFRASEGKISAIKYARENNVPFFGICLGMQLATVEF
                     SRNVLGLEGAHSAELDPATPYPIIDLLPEQKDIEDLGGTLRLGLYPCSIKEGTLAQDV
                     YGKAEIEERHRHRYEFNNDYREQLEANGMVISGTSPDGRLVEVVEIPTNDFFIACQFH
                     PEFLSRPNRPHPIFKSFIEASLKYQQNK"
     gene            complement(2000..2530)
                     /locus_tag="O0293_002311"
     CDS             complement(2000..2530)
                     /locus_tag="O0293_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HCY0281182.1"
                     /translation="MKIQDYTKQMVDEKSFIDMAYTLLNDKGETMNLYDIIDEFRALG
                     DYEYEEIENRVVQFYTDLNTDGRFLNVGENLWGLRDWYSVDDIEEKIAPTIQKFDILD
                     ADDEEDQNLKLLGEDEMDDDDDIPAQTDDQEELNDPEDEQVEEEINHSDIVIEEDEDE
                     LDEDEEVFEDEEDFND"
     gene            complement(2642..3502)
                     /locus_tag="O0293_002312"
     CDS             complement(2642..3502)
                     /locus_tag="O0293_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0281183.1"
                     /translation="MAKELCFEGITLKAFDEQYRSAINDFDLNERQQIYSSLPKEVID
                     DAINDADRIANVAINDKNEVVGFFVLHRYYQHEGYDTPENVVYIRSLSINEKYQGFGY
                     GTKIMMSLPQYVQGVFPDFNHLYLVVDAENDNAWNLYERAGFMHTATKEEGPIGKERL
                     YYLDLDSKHVSSLKLEEESRSEVTNVHIINLMIDGQKVGFIALEQIGERMNIAAIEVD
                     KSYRFNGIGSSALRQLPTYLRKNYDNLNVITMILFGENNDFKPLCLNSNFVEIEQTDD
                     YVVFEKYLNY"
     gene            3926..4729
                     /gene="coaW"
                     /locus_tag="O0293_002313"
     CDS             3926..4729
                     /gene="coaW"
                     /locus_tag="O0293_002313"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II pantothenate kinase"
                     /protein_id="HCY0281184.1"
                     /translation="MKVGIDAGGTLIKIVQEQDNQRTFKTELTKNIDQVVEWLNQQQI
                     EKLCLTGGNAGVIAENINIPAQIFVEFDAASQGLGILLKEQGHDLADYIFANVGTGTS
                     LHYFDGQSQRRVGGIGTGGGMIQGLGYLLSQITDYKQLTDMAQHGDRNTIDLKVRHIY
                     KDTEPPIPGDLTAANFGHVLHHLDADFTPSNKLAAVIGVVGEVVTTMAITVAREFKTE
                     NIVYIGSSFHNNALLRKVVEDYTVLRGCKPYYVENGAFSGAIGALYLEK"
     gene            complement(4859..>5464)
                     /locus_tag="O0293_002314"
     CDS             complement(4859..>5464)
                     /locus_tag="O0293_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2750 domain-containing protein"
                     /protein_id="HCY0281185.1"
                     /translation="KNKKLVRLNYKSEAYVGVWTEESVAVSFLTSRDIPFDKVVKMDV
                     DRFATYELDELFDEQDHIIMNQTMEEEGHLLNVVAVTQEVMTELDKIRIKEFVQDVAK
                     YDEVYGLTKKGSKQFILISENDSDEKKPHIMPVWSIKNRALKVRDEDFEECDLITIEG
                     SVFGEWLDELRDDHKAVAIDLKTGVVGTIVSAQKLSNELTF"
BASE COUNT         1719 a         1039 c          747 g         1959 t
ORIGIN      
        1 aaaaccggta ctttcaatgt ttaacaatga aagtaccggt tttattagca aatttattta
       61 ttttgttgat attttaatga agcttcaata aatgatttaa aaatcgggtg cggacgattt
      121 ggtctagata agaattctgg gtggaattga caagcaataa agaaatcatt tgtcggaatc
      181 tctaccactt ctactaaacg tccatctgga cttgtaccag aaatcaccat accatttgct
      241 tctaattgtt ctctatagtc attattaaat tcataacgat gacgatgtct ttcttcaatt
      301 tccgctttac cataaacatc ttgtgccaat gtgccttctt taattgaaca tggatataag
      361 cctaagcgta atgtaccacc taaatcttcg atatcttttt gttctggtaa taaatctata
      421 attgggtatg gtgttgctgg gtctaattca gctgaatgtg cgccttcaag gcctaatacg
      481 tttcttgaaa attcaactgt tgcaagttgc attcctaaac aaataccaaa gaatggtaca
      541 ttgttttctc tagcatactt aattgcacta attttacctt cacttgcacg gaaaccaaat
      601 ccacctggta ctaaaatacc gtcgacatct gcaaggtatt cggctgcatt ttcatctgtt
      661 acttcacttg aatcaatcca tctaatgtca atatctttgg caaaaggata tccagcatgt
      721 ttcaatgatt caacaactga taaatatgca tcttgtaagc taacatattt acctactaaa
      781 ccaattgtaa ttttaccatc taaattatta acgatatcta acaactgttt ccattcatca
      841 agctgtgttt catatttcgc gtttaattgt aaacgtttaa taacgatatc atccatattt
      901 tgttggctta attgtaatgg aatttcgtat aaagagtctg catcacgaca ttcaataaca
      961 ctttctttat taatgtcaca gaataatgca attttatctt ttaaatcttg tgtcatttca
     1021 tattcagttc ttacaacgat taagtctggt tgaataccta agcctcgtaa ttctttaaca
     1081 ctatgttgtg ttggcttcgt tttcatttct ccagcagctt taatataagg cagtaatgta
     1141 cagtgaacat acataacatt ttctctacct aaatcgctac gaatttgacg aatcgcttca
     1201 ataaacggta atgactcaat atcacctgtt gtaccgccaa tttcagtgat aacaacgtct
     1261 gcattcgtac tttcccctgc aagtaataaa cgttctttaa tttcatttgt aatatgcgga
     1321 ataacttgaa ctgttccgcc taagtaatca ccacgacgtt cttttttcaa tacgtgtgaa
     1381 tacactttac cggctgtcac atttgaaaac ttgtttaaat taatatcaat aaatctttcg
     1441 taatgtccta agtctaggtc agtttctgca ccatcatccg ttacgaatac ttcaccatgt
     1501 tgataaggac tcattgtacc tgggtcaaca tttaagtatg gatcgaattt ttgaattgtt
     1561 acatttagac ctctatcttt taataatcta cctagagaag atgctgtaat acccttccct
     1621 aatgatgaaa ctacgccacc tgttacaaaa ataaattttg tcatgtcttg acctcctaat
     1681 tattaaaggg aatgcttcct catcaattgt aaaaattacg taaaacataa cattaaagca
     1741 agctcattca agttgtttat ttgtcgaatt cgacaaaatt aaattctgtt tacttaccgt
     1801 ttccatactg ctatgtcact atgtatatca tttacaactt tgatttttgc gttatcattt
     1861 cttgatttac tcaattaaat aaaaaaacgc tccctctcac aaattctgta aggggagcgt
     1921 ataaatttta tacacgtgtc ctatttaaag gagcccgaat aaaattttat catctttcaa
     1981 ctaaaagtca aacaaaaaat taatcgttga agtcttcttc gtcttcaaac acttcttcgt
     2041 cttcgtctag ttcatcttca tcttcttcaa tgactatatc cgaatgattg atttcttctt
     2101 caacctgctc atcttctgga tcatttagtt cttcttgatc atctgtttga gctggaatat
     2161 catcgtcgtc atccatttca tcttcgccca ataattttaa gttttgatct tcttcatcat
     2221 ctgcatccag aatatcgaat ttttgaatag ttggtgcgat tttctcttca atatcatcta
     2281 ccgaatacca atcacgtaat ccccataaat tttctccaac atttaaaaaa cgaccatctg
     2341 tgtttaaatc cgtgtaaaat tgtacaacac gattttcaat ttcttcgtac tcataatcac
     2401 ctaacgctct aaattcatcg atgatatcat ataagttcat tgtttcgcct ttatcattca
     2461 ataatgtata agccatatca ataaatgatt tttcatcaac catttgtttt gtataatctt
     2521 gaattttcat tgcccaatac ttcctttcga aggatataat ctaaatttat acgtctgaat
     2581 tcatttatat atagtaacaa aactaaatta taaatgacaa tatcatattt cgcaatcact
     2641 gttagtaatt taaatatttt tcgaaaacga cataatcatc agtttgttcg atttcaacga
     2701 aattactatt taaacataat ggtttaaaat cattattctc tccaaacaga atcatcgtaa
     2761 tcacattaag gttgtcatag ttttttctta agtaagttgg caattgtcgc agagcacttg
     2821 aaccaatacc attaaagcga tatgatttat ccacttcaat agcagcaatg ttcatgcgtt
     2881 caccaatctg ctccaatgcg ataaagccaa ccttttggcc atcaatcatt aaattaatga
     2941 tatgtacatt ggtcacttct gaacgacttt cttcttcaag ctttaatgat gaaacatgtt
     3001 ttgaatctaa gtccaagtaa tatagtcgtt ctttgccaat tggtccttct tctttagtcg
     3061 ctgtatgcat gaatccagca cgttcgtata ggttccaagc attgtcattt tccgcatcta
     3121 ctactagata tagatgatta aaatcaggaa atacaccttg aacatattgc ggcaatgaca
     3181 tcattatttt cgtgccatat ccaaaacctt gatatttttc attaatcgat aatgaacgaa
     3241 tataaacgac attttcaggt gtatcataac cttcatgctg atagtaacga tgtaatacaa
     3301 aaaagcccac cacttcattt ttatcgttta ttgctacgtt agcaatccta tcagcatcat
     3361 taattgcatc atcaataact tctttaggta aagatgaata tatttgttgt ctttcattca
     3421 agtcaaaatc attaattgct gaacgatatt gttcatcaaa tgcttttaaa gtgatacctt
     3481 caaaacaaag ttctttagcc atatatttaa cccctatctt tttatatatg aagcatgaat
     3541 ccccatatta ttttacctat tatttcacta cgataccatt atttgctcta agttaaataa
     3601 attacttatt tatatttttt aaatttcaaa attaatgtac tattcctcat tacttatcat
     3661 tttaatcaga gacttacaaa tacactgcgt acgaactact tttaatattg cgtcactaac
     3721 taacaatcct atacaacttc aaaattccta gaaaataata tatataactt aacgtccacg
     3781 tttgtaaaac attaattgcg atacgcttac aatgttgaaa tgcaatggca tcaaatttgt
     3841 taataattac tattattact tgttttttag tcattttact atactattat ttaaggatag
     3901 acattaataa tgaggtggga atcttatgaa agttggcatt gacgctggcg gtacactaat
     3961 taaaatcgtt caagagcaag ataaccaacg tacttttaaa actgaattaa ctaaaaatat
     4021 tgatcaagtt gtggaatggt taaaccaaca gcaaattgaa aaattatgct taactggagg
     4081 taatgcaggt gtcattgctg aaaacatcaa cattcctgca caaatttttg tagagtttga
     4141 tgctgcatct caaggactag gtattttgtt aaaagagcaa ggccatgact tagcagatta
     4201 tatttttgca aatgttggta ccggtacgtc actacattat ttcgacggcc aatcacaacg
     4261 tcgtgtaggc ggtatcggta caggtggagg catgattcaa ggactcggtt atttactaag
     4321 tcaaatcact gattataaac agttaactga catggcacaa cacggtgatc gtaacactat
     4381 cgatttaaag gttagacata tttataaaga taccgaacca cccattcccg gtgatttaac
     4441 agcagctaat tttggtcatg tactacatca tttagatgct gattttacac ctagcaataa
     4501 attagcagcc gttattggtg tcgttggcga agttgttacg acaatggcca ttactgttgc
     4561 acgtgaattt aaaactgaaa atatcgttta tatcggttct tcatttcata ataacgcttt
     4621 attacgcaaa gttgtggaag attatactgt gttacgcggt tgcaaaccgt actatgttga
     4681 aaacggcgca ttttcaggtg ctattggtgc attatattta gaaaaataga aaaagaccat
     4741 ttatatgcgc gttgttttaa ataaacgcta tataaatggc cttttatttt atggtctaag
     4801 tgttttgaat acgtattgtt ccatttatta tcacttaatt acttacaact caattcaatt
     4861 aaaatgtaag ttcattagac aatttttgcg ctgaaacaat tgtaccaacc acgccagttt
     4921 ttaaatctat cgcaacggct ttatgatcat ctctaagttc atctagccat tctccgaaaa
     4981 cagaaccttc aatcgtaatt aaatcacact cttcaaaatc ttcatctcga acttttaacg
     5041 ctctgttttt aatactccat acaggcataa tatgcggctt tttttcgtcg ctatcatttt
     5101 cactaatgag aataaactgc ttactacctt ttttagttaa gccgtatact tcatcatatt
     5161 tcgctacatc ttggacaaat tctttgattc taattttatc taattccgtc atcacttctt
     5221 gtgtaacagc tacaacgttt agtagatgcc cttcttcttc cattgtttga ttcataataa
     5281 tatggtcttg ttcatcaaac aattcatcta attcataagt agcaaaacga tcaacgtcca
     5341 tttttacaac tttatcaaat ggaatatcac gacttgttaa aaatgatacg gccacacttt
     5401 cttctgtcca aacgcctaca tacgcttcac ttttataatt taggcgtact agttttttat
     5461 tttt
//
LOCUS       DAKTIO010000166         5447 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.168, whole genome shotgun sequence.
ACCESSION   DAKTIO010000166 DAKTIO010000000
VERSION     DAKTIO010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5447
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.168"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(175..1014)
                     /locus_tag="O0293_002315"
     CDS             complement(175..1014)
                     /locus_tag="O0293_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0281186.1"
                     /translation="MKKSLTVTVSSVLAFLALNNAAHAQQHGTQVKTPVQHNYVSNVQ
                     AQTQSPTTYTVVAGDSLYKIALEHHLTLNQLYSYNPGVTPLIFPGDVISLVPQNKVKQ
                     TKAVKSPVRKASQAKKVVKQPVQQASKKVVVKQAPKQAVTKTVNVAYKPAQVQKSVPT
                     VPVAHNYNKSVANRGNLYAYGNCTYYAFDRRAQLGRSIGSLWGNANNWNYAAKVAGFK
                     VDKTPEVGAIFQTAAGPYGHVGVVESVNPNGTITVSEMNYAGFNVKSSRTILNPGKYN
                     YIH"
     gene            complement(1162..1269)
                     /locus_tag="O0293_002316"
     CDS             complement(1162..1269)
                     /locus_tag="O0293_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281187.1"
                     /translation="MCLLKQHMQCAFSNITYGDFQGIELIIENVLNYMV"
     gene            complement(1423..2533)
                     /gene="prfB"
                     /locus_tag="O0293_002317"
     CDS             complement(join(1423..2460,2462..2533))
                     /gene="prfB"
                     /locus_tag="O0293_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667688.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCY0281188.1"
                     /translation="MELSEIKRNIDKYNQDLTQIRGSLDLENKETNIQEYEEMMAEPN
                     FWDNQTKAQDIIDKNNALKAIVNGYKTLQAEVDDMDATWDLLQEEFDEEMKEDLEQEV
                     INFKAKVDEYELQLLLDGPHDANNAILELHPGAGGTESQDWANMLFRMYQRYCEKKGF
                     KVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFAS
                     CDVIPDFNNDEIEIEINPDDITVDTFRASGAGGQHINKTESAIRITHHPSGIVVNNQN
                     ERSQIKNREAAMKMLKSKLYQLKLEEQAREMAEIRGEQKEIGWGSQIRSYVFHPYSMV
                     KDHRTNEETGKVDAVMDGDIGPFIESYLRQTMSHD"
     gene            complement(2702..2806)
                     /locus_tag="O0293_002318"
     CDS             complement(2702..2806)
                     /locus_tag="O0293_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281189.1"
                     /translation="MLWEESFYFTSVKYFKKCEEVVKRCYVLSFKKSV"
     gene            complement(2848..5379)
                     /gene="secA"
                     /locus_tag="O0293_002319"
     CDS             complement(2848..5379)
                     /gene="secA"
                     /locus_tag="O0293_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCY0281190.1"
                     /translation="MGFLSKILDGNNKEIKQLGKLADKVIALEEKTAILTDEEIRNKT
                     KQFQTELADIDNVKKQNDYLDKILPEAYALVREGSKRVFNMTPYKVQIMGGIAIHKGD
                     IAEMRTGEGKTLTATMPTYLNALAGRGVHVITVNEYLSSVQSEEMAELYNFLGLTVGL
                     NLNSKTTEEKREAYAQDITYSTNNELGFDYLRDNMVNYSEDRVMRPLHFAIIDEVDSI
                     LIDEARTPLIISGEAEKSTSLYTQANVFAKMLKQDEDYKYDEKTKAVHLTEQGADKAE
                     RMFKVENLYDVQNVDVISHINTALRAHVTLQRDVDYMVVDGEVLIVDQFTGRTMPGRR
                     FSEGLHQAIEAKEGVQIQNESKTMASITFQNYFRMYNKLAGMTGTAKTEEEEFRNIYN
                     MTVTQIPTNKPVQRNDKSDLIYISQKGKFDAVVEDVVEKHKAGQPVLLGTVAVETSEY
                     ISNLLKKRGIRHDVLNAKNHEREAEIVAGAGQKGAVTIATNMAGRGTDIKLGEGVEEL
                     GGLAVIGTERHESRRIDDQLRGRSGRQGDKGDSRFYLSLQDELMIRFGSERLQKMMSR
                     LGLDDSTPIESKMVSRAVESAQKRVEGNNFDARKRILEYDEVLRKQREIIYNERNSII
                     DEEDSSQVVDAMLRSTLQRSINYYINTADDEPEYQPFIDYINDIFLQEGDITEDDIKG
                     KDAEDIFEVVWAKIEAAYQSQKDILEEQMNEFERMILLRSIDSHWTDHIDTMDQLRQG
                     IHLRSYAQQNPLRDYQNEGHELFDIMMQNIEEDTCKFILKSVVQVEDNIEREKTTEFG
                     EAKHVSAEDGKEKVKPKPIVKGDQVGRNDDCPCGSGKKFKNCHGK"
BASE COUNT         1590 a         1053 c          778 g         2026 t
ORIGIN      
        1 tttcaattac ctcctccatt aattaatcat aaattatact ttctttttac atatcaatca
       61 attaaatatc atttaaatat cttctttata taactctgat taaatgatac caaaaaatcc
      121 tctcaacctg ttacttaaac aggctaagag gatagtcttg tcttgatata ttacttagtg
      181 gatgtaatta tattttcctg gatttaaaat tgttcttgaa gatttaacat taaatccagc
      241 atagttcatt tcagaaacag taattgttcc attagggttt acagattcaa caacaccaac
      301 atgtccatat ggaccagcag ctgtttggaa aatagcgcca acttctggtg ttttatctac
      361 tttaaatcct gcaacttttg ctgcgtaatt ccagttattt gcattgcccc ataaacttcc
      421 tatacttcta cctaattgtg cacgacgatc gaaagcataa tatgtgcagt ttccataagc
      481 atataagttt cctctgttag caactgattt attgtagtta tgtgcaacag gtacagttgg
      541 tactgatttt tgtacttgag caggtttgta tgctacatta actgtcttag ttactgcttg
      601 cttaggtgct tgcttaacta ctactttttt agatgcttgt tgtacaggtt gttttactac
      661 ctttttagct tggcttgctt ttcttactgg tgatttaacc gctttagttt gtttcacttt
      721 attttgaggc acaagtgaaa tcacgtcacc aggaaaaatt aaaggtgtta caccaggatt
      781 gtatgaatat aattgattca acgttaagtg atgctctaaa gcaatcttat ataatgaatc
      841 gccagcaact actgtataag ttgtcggtga ttgcgtttgt gcttgaacat ttgatacata
      901 attatgttga acaggtgttt ttacttgtgt gccatgttgt tgtgcatgtg ctgcattatt
      961 taaagctaaa aaagctaaca ctgacgaaac cgtcactgta agagattttt tcatcttgct
     1021 gtcattcctt tgctgttagt attttaagta tgcaaatact atagcacaat acattttgtc
     1081 aaaagctatt gttataacga tgtaatcaaa tggttaacaa tataaaaaga atacaacctt
     1141 ttatcatagt gtaaaatgta ttcataccat gtaattgaga acgttttcaa taattaattc
     1201 aataccttga aaatcgccat aggtaatatt actaaatgca cactgcatat gttgttttaa
     1261 caaacacaac ttttaaaaaa tatattctaa ctctatctac cgaattgtac ttaaatattc
     1321 ataaacaaat catattccaa aatctaattt acaatttatt tagctacctt taaaaaaacc
     1381 aaaaaccgac gcccttttag agcctcggtt ttaaaatata tattaatcgt gcgacattgt
     1441 ctgtcttaaa tatgattcga taaatggtcc aatgtctcca tccatcactg catcaacctt
     1501 acctgtttct tcgttcgtac gatgatcttt caccattgag tatggatgga aaacatatga
     1561 tctaatttgg cttccccagc cgatttcttt ttgttcgcca cgaatttcag ccatttcacg
     1621 tgcctgctct tccaatttta attgatataa tttagacttt aacattttca tagctgcttc
     1681 acggttttta atttgagaac gttcattttg gttattaaca actatacctg aggggtggtg
     1741 ggtaattcgt attgccgatt cagttttgtt aatatgctga ccacctgcac cagaagctct
     1801 gaatgtatca actgtaatat catccggatt gatttcaatc tctatttcat cattattaaa
     1861 atctggaata acgtcgcatg atgcaaatga tgtatgacga cgtcctgatg aatcaaatgg
     1921 agaaattcgt actagtcggt gtacaccttt ttcagctttt aaataaccat aagcattatg
     1981 ccctttgatg agcaatgtta cacttttaat ccccgcttca tccccaggta gataatcaac
     2041 agtttcaact ttaaagcctt tcttctcaca ataacgttga tacattctaa atagcatatt
     2101 agcccaatct tgagactccg tgccacctgc accaggatgt aactctagaa ttgcgttatt
     2161 ggcatcgtga ggcccatcta ataataattg caattcgtat tcatccactt tagccttaaa
     2221 attaatgacc tcttgctcta agtcttcttt catttcttca tcaaattctt cttgtaataa
     2281 atcccaagta gcatccatgt catctacttc tgcttgtagt gttttataac cattaactat
     2341 tgcttttaac gcattatttt tatctataat atcttgcgct ttcgtttggt tatcccaaaa
     2401 attaggttct gccatcattt cttcatattc ttgaatatta gtttctttgt tctctaagtc
     2461 aaagagaccc cctaatttgt gttaaatctt gattatactt atctatattt cgtttgattt
     2521 ctgataattc catagcattc gctcctattt atatttcaat tcaagtcatt gatttgcatc
     2581 ttttataatg ctaaatttta acataatttt gttaaataac aatgttaaga aatataagca
     2641 cactgacaat tagtttatgc atttattgtt aaaaatcagt acatttatca tcgacatatg
     2701 cctaaaccga ttttttaaaa ctaagtacat aacaacgttt aacaacttct tcacattttt
     2761 taaagtattt aacgcttgta aaataaaaag actcctccca taacacaaac tataggtgtt
     2821 taattggaag gagttatttt atatcattta ttttccatgg caatttttga attttttacc
     2881 actaccacat ggacaatcat cgttacgacc aacttgatcg cctttaacga ttggtttcgg
     2941 tttcactttt tctttaccat cttcagctga aacgtgcttc gcttcaccaa actctgttgt
     3001 tttttcacgt tcaatattat cttcaacttg tactacagat tttaaaatga atttacaagt
     3061 atcttcttca atattttgca tcatgatatc aaataattca tgaccttcat tttgatagtc
     3121 acgtaatgga ttttgttgtg cataagaacg taagtgaata ccttgacgta attgatccat
     3181 tgtgtcgata tgatcagtcc aatggctatc aatagaacga agtaaaatca tacgctcaaa
     3241 ctcattcatt tgttcttcta agatatcttt ttgactttga tatgctgctt caatcttagc
     3301 ccaaacgact tcgaaaatat cttcagcatc tttacctttg atatcatcct ctgtaatgtc
     3361 accttcttgt aagaagatgt cattaatgta gtcgatgaat ggttgatatt caggctcgtc
     3421 atctgctgta ttaatatagt aattgatact acgttgtaac gttgaacgta gcattgcatc
     3481 tacaacttga gagctgtctt cttcatcaat aatactattt ctttcgttat agataatttc
     3541 acgttgttta cgtaatactt catcgtattc taagatacgt ttacgcgcgt cgaagttatt
     3601 accttctaca cgtttttgtg ctgattctac agctcttgat accatttttg attcaattgg
     3661 tgtagagtca tctaaaccta gtcggctcat cattttctgt aaacgttcag aaccaaaacg
     3721 aatcattaat tcatcttgta atgataaata gaagcgacta tcccctttat caccttgacg
     3781 tccagaacga ccacgtaact ggtcatcaat acgacgagat tcatgtcgct ctgtacctat
     3841 tactgctaaa ccgcctaatt cctctacgcc ttcacctaat ttgatatctg taccacgacc
     3901 agccatgtta gtggcaatag taacggcacc tttttgtcca gcgcctgcaa caatttcagc
     3961 ttcacgttca tgatttttcg catttaacac atcatgacgg ataccacgtt ttttaagtaa
     4021 atttgaaata tattcagaag tctcaactgc aacagtacct aatagcactg gttgccctgc
     4081 cttgtgtttt tcaacaacat cttctactac tgcatcaaat ttaccttttt ggctaatgta
     4141 aattaaatca gacttatcgt tacgttgcac aggtttattt gtcggaattt gagttactgt
     4201 catgttataa atatttctaa attcttcttc ttcagtttta gctgtacctg tcatacccgc
     4261 aagtttattg tacattctga aatagttttg gaatgtaata gacgccatag ttttagattc
     4321 attttgaatt tgaacgcctt ccttcgcttc aatagcttgg tgtaaacctt ccgagaaacg
     4381 acggcctggc attgtacgtc ctgtaaattg atcgacaatt aatacttcgc catcaacaac
     4441 catatagtct acgtcacgtt gtaatgtaac gtgcgcacgt aaagctgtgt tgatatgact
     4501 aataacatca acattttgta catcatataa gttttcaact ttgaacatac gttcagcttt
     4561 atccgcacct tgttctgtta aatgtacagc tttcgttttt tcatcgtatt tataatcttc
     4621 gtcctgtttt aacattttcg caaaaacatt tgcttgtgta taaagtgacg ttgacttttc
     4681 agcttcacca gaaataatta atggcgtacg tgcctcgtcg attaaaattg agtcaacctc
     4741 atcaatgatt gcaaaatgta atggacgcat tactctatct tcagaataat tcaccatgtt
     4801 atctcgtaag taatcaaaac ctagctcatt attagtactg taagtaatgt cttgtgcgta
     4861 tgcttcacgt ttttcttctg tcgtcttact gtttaagttt aatccgacag tcaaacctaa
     4921 gaagttatat aactcagcca tttcttcact ttgaacactt gataagtatt cattgactgt
     4981 aataacgtga acacctctac cagctaatgc atttaagtat gttggcattg tcgctgttaa
     5041 tgttttacct tcacctgttc tcatctcagc gatatcacct ttatgaattg caataccacc
     5101 cataatttga actttatatg gtgtcatatt gaatacacgt ttagagcctt ctctaacaag
     5161 tgcatatgct tctggtaaaa ttttatctaa ataatcattt tgctttttga cattatcaat
     5221 gtcagctaat tctgtttgga attgtttcgt tttattacga atttcttcat cagttaaaat
     5281 tgccgttttt tcttctaaag cgattacttt atcagcaagt ttacctaact gtttaatttc
     5341 tttattattg ccatcaagaa tttttgataa aaatcccatt tcgttcgctc ctttagctaa
     5401 aaaactgttt ggcctacaac aatatatctt atcatttata gttagaa
//
LOCUS       DAKTIO010000167         5374 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.169, whole genome shotgun sequence.
ACCESSION   DAKTIO010000167 DAKTIO010000000
VERSION     DAKTIO010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.169"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            180..1301
                     /locus_tag="O0293_002320"
     CDS             180..1301
                     /locus_tag="O0293_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001662816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HCY0281191.1"
                     /translation="MAELQRGLEGVIAAETKISSIIESQLTYAGYDIDDLAENAQFEE
                     VIFLLWNYRLPNEEELAHLKGKLNQYMTLNPRVYTHFEEYVTDHVHPMTALRTSLSYI
                     AHFDPDAENESDENRYERAMRIQAKVASLVTAFARVRQDKEPLKPNPDLSYAANFLYM
                     LRGELPTDIEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIVAAVGSLKGPL
                     HGGANEQVMTMLSEIGSIENVDAYLDEKFANKDKVMGFGHRVYKDGDPRAKYLREMSR
                     QITKDAGREELFEMSVKMEKRMAEEKGLIPNVDFYSASVYHCMEIPHDLFTPIFAVSR
                     SAGWIAHILEQYKDNRIMRPRAKYIGETNRKYIPLEERK"
     gene            1350..2618
                     /gene="icd"
                     /locus_tag="O0293_002321"
     CDS             1350..2618
                     /gene="icd"
                     /locus_tag="O0293_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCY0281192.1"
                     /translation="MTAEKITQGTEGLNVPNEPIIPFIIGDGIGPDIWKAASRVIDAA
                     VEKAYNGEKRIEWKEVLAGQKAFDTTGEWLPQETLDTIKEYLIAVKGPLTTPIGGGIR
                     SLNVALRQELDLFTCLRPVRWFKGVPSPVKRPQDVDMVIFRENTEDIYAGIEFKEGTT
                     EVKKVIDFLQNEMGATNIRFPETSGIGIKPVSKEGTERLVRAAIQYAIDNNRKSVTLV
                     HKGNIMKFTEGSFKQWGYDLALSEFGDQVFTWQQYDEIVENEGRDAANAAQEKAEKEG
                     KIIIKDSIADIFLQQILTRPAEHDVVATMNLNGDYISDALAAQVGGIGIAPGANINYE
                     TGHAIFEATHGTAPKYAGLNKVNPSSVILSSVLMLEHLGWQEAADKITDSIEDTIASK
                     VVTYDFARLMDGAEEVSTSAFADELIKNLK"
     gene            3008..3712
                     /locus_tag="O0293_002322"
     CDS             3008..3712
                     /locus_tag="O0293_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0281193.1"
                     /translation="MSQKVLVVDDEHSIVTLLKYNLETAGYEVVVAFDGDEALEKVES
                     EQPDLIILDVMLPKKDGIDVCKTVRTNKNLVPILMLTAKNDEFDRVLGLELGADDYMT
                     KPFSPREVVARVKAILRRSQFVKEVEEIDDDITIGSIRIRPDYFEVYKHNELLELTPK
                     EFELLLYLIERQGRVITREHMLNTVWNYEFAGDSRIVDVHISHLRDKLEDNPKKPQLI
                     KTVRGLGYKLERPKEQ"
     gene            3712..5373
                     /locus_tag="O0293_002323"
     CDS             3712..5373
                     /locus_tag="O0293_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HCY0281194.1"
                     /translation="MKFHHRLMLLISSILIISFVTLGIIIHASITYVLSEKHEQALAT
                     DARSYIYLVQDNEIDKVKKIVKQQNIDLLITKHDKKVFSSGKLKAIPNSDSYIQKRDV
                     FIFNKKVNGYHVWIKGYNNDITEMHWTLWKYLILTCLVVLICLYFASRSFKRTLIRPI
                     QEVTYATQLLANGYYHIRVPESNVVETKALFVSTNDLARRLQKLNNEQKIQSNRLKTT
                     IENIPSAILMIDRNGKIVVANKAYYEQFNISHNIEQVGYHGYVNTEIEQLILESFKVE
                     KPIYEQLEVAINQVHAKYFDISCVPILTRSQKSLQGILVVMHDITNLKQLENLRREFV
                     ANVSHELKTPITSIKGFAETLIDGAKNDAESLDMFLNIILKESNRIESLVTDLLDLSH
                     IEQHTELDTDYMNLSDLTRRIIDNMMTQANQKNISIHTDIEKDVIVKAQESKIAQVIT
                     NLLTNAINYSYEDGDINVRVYRDDFRVIFEVQDFGIGIKLEDQQRIFERFYRVDKARS
                     RDSGGTGLGLSITKHIVEAHQGNIEVNSQVGKGSTFKVILKDYKE"
BASE COUNT         1950 a          733 c         1048 g         1643 t
ORIGIN      
        1 tcacatcttg aaaaagcttt aatggtgcta tttgtggcta ttctgtgaca tttacataga
       61 tttacaaaaa aattgttgca catataatgc cagtttttat atttcacaaa cgaaatgcgt
      121 ttagctataa tattagttga aagccatttc ataaagaaac agtaaagggg aaatttatca
      181 tggcagaatt acaaagaggt ttagaagggg ttatcgcagc ggagactaaa ataagttcaa
      241 ttattgaaag tcaattgact tatgccggct atgatattga tgatctagct gaaaatgcgc
      301 aatttgaaga agttattttc ctattatgga actatagatt gccaaacgaa gaagagcttg
      361 ctcatctaaa agggaaatta aatcaataca tgacattaaa tcctcgtgtg tatacacatt
      421 ttgaggagta tgttacagat cacgtgcatc caatgacagc attacgtacg tcattatcat
      481 atattgcaca tttcgatcct gatgctgaaa atgaatcaga tgaaaatcgt tatgaaagag
      541 caatgcgtat acaggctaaa gtagcatcat tagttacagc gtttgctcga gtaagacaag
      601 ataaagaacc acttaagcct aatcctgact taagttatgc ggcaaacttc ctatatatgt
      661 tacgtgggga attaccaaca gatatagaag tagaagcctt caataaagca cttattttac
      721 acgctgatca tgagttgaac gcatctgcat ttacggcacg ttgtgcggta tcatcattgt
      781 cagatatgta ctcaggtatt gtagcagccg taggttctct gaaagggcca ttacatggtg
      841 gtgcaaacga acaagttatg acgatgttat ctgagattgg gtcaattgaa aatgttgatg
      901 cttacttaga tgaaaaattt gctaataaag ataaagtaat gggcttcggt catcgtgtat
      961 ataaagatgg tgatcctaga gcgaaatatt taagagaaat gagccgtcaa attacgaaag
     1021 acgctggtcg tgaagaatta tttgaaatgt cagtgaaaat ggaaaaacgt atggcagaag
     1081 aaaaaggatt aattcctaat gttgattttt atagtgcgag tgtttatcac tgtatggaaa
     1141 tacctcatga cttattcacg ccaatctttg ctgtaagtcg ttctgcagga tggattgctc
     1201 atattttaga acaatataaa gataatagaa ttatgcgtcc tagagcgaaa tatattggcg
     1261 aaacgaatcg taagtatatc ccgcttgaag aaagaaaata atcaatacaa attaaaaatg
     1321 aagatgtaaa atttggaggt aaaataacta tgactgcaga aaaaattact caaggaactg
     1381 aaggattaaa cgtacctaat gaaccaatta tcccatttat tatcggtgat ggaattggac
     1441 cggatatttg gaaggcagca agccgagtta tagatgctgc tgttgagaaa gcctataatg
     1501 gcgaaaaacg cattgaatgg aaagaagtgc tagctggcca aaaagcattt gatacaactg
     1561 gtgaatggtt acctcaagaa acacttgata caattaaaga atatttaatt gctgttaaag
     1621 gacctttaac aacaccaatt ggtggtggta ttagatcatt aaatgtggct ttacgccaag
     1681 aattagattt atttacttgc ttaagaccgg tacgttggtt taaaggagta ccatcacctg
     1741 ttaaacgtcc acaagatgtt gatatggtta ttttccgtga aaatactgaa gacatttatg
     1801 ctggtattga atttaaagaa ggtacaacag aagttaaaaa ggtaattgac ttcttacaaa
     1861 acgaaatggg tgcgacaaac attcgattcc cagaaacttc aggtattggt attaaaccag
     1921 tttctaaaga aggaactgag cgattagtta gagcagctat acaatatgct atcgataata
     1981 accgtaaatc agttacttta gttcataaag gtaatattat gaaatttaca gaaggctcat
     2041 ttaagcagtg gggttacgat ttagcattat ctgaatttgg tgatcaagta ttcacttggc
     2101 aacaatatga cgaaattgtt gaaaatgaag gcagagatgc tgctaatgct gctcaagaaa
     2161 aagctgaaaa agaaggcaag attatcatta aagattctat tgctgacatt ttcttacaac
     2221 aaattttaac tcgtccagct gagcatgatg ttgtagcaac tatgaacttg aatggtgact
     2281 atatttcaga tgctttagct gcacaagttg gtggtattgg tattgcgcca ggtgcaaaca
     2341 ttaattatga aacaggtcat gctatttttg aagcaacaca tggtacagct ccaaaatatg
     2401 caggtttaaa taaagtgaat ccatcttcag taattttaag ttctgtatta atgttagaac
     2461 atttaggatg gcaagaagcg gcagataaga ttacagattc aattgaagat acaattgctt
     2521 caaaagttgt tacttatgac tttgcccgtt taatggatgg tgctgaagaa gtttctacat
     2581 cagcatttgc agatgaattg attaaaaatt taaaataagc aaaataaaat taggtgagtc
     2641 tagcaacttt aattgctagg ttcgcctttt taaatgaatt gaagttggca acaggataga
     2701 aataataaag agtcaccaat gattgattat gtagtgtttc ttacacatta gacaaagcga
     2761 atatgtacat aagcttagga ataaatgagt aaaactcatg cataggtagt gggatagaaa
     2821 tgctattttt acaaaattta tttcgttgtc ccagagtaag tcatgcaaat gatagaagga
     2881 aatataagaa gtattttatt tatatgtaaa gtaatgtaaa aaatgtgtaa agataatgtg
     2941 taggatgtta tttttataaa tagttcggtt ataatgtaaa tataattata agttagggag
     3001 gcataccatg tcgcaaaaag tgttggtagt agatgacgaa cattcaatcg taacattact
     3061 taaatataac ttagaaacag ctggttatga agttgttgtc gcatttgatg gtgatgaggc
     3121 tttagaaaag gtagaaagtg aacagccaga tttaattatt ttagatgtta tgctacctaa
     3181 aaaagatggc attgacgtat gtaagactgt aagaacaaat aaaaatttag tccctatatt
     3241 aatgttaacg gcgaaaaatg atgaatttga tcgggtatta ggtttagaat taggtgccga
     3301 tgactatatg actaagcctt tttcacctag agaagttgtt gcccgtgtga aagccatttt
     3361 aagacgttct caatttgtaa aagaagtaga agagattgac gatgatatta ccatcggatc
     3421 gatacgtatt agacccgatt attttgaagt atataagcat aatgagttat tggagctaac
     3481 accgaaagaa tttgaactct tgctttattt gatagaaaga caaggcagag tcattacgag
     3541 agagcatatg ttaaatacgg tatggaatta tgaatttgca ggagactcaa gaattgttga
     3601 tgtacatatc agtcatttaa gagataaatt agaagataat cctaagaagc cacaacttat
     3661 taaaactgtg cgtggcttag gctataaatt ggaaagacct aaagaacaat gatgaagttt
     3721 caccaccgct taatgttatt gataagcagt atattaatta tcagttttgt tacattaggc
     3781 attattattc acgcttccat cacatatgtt ttatcagaaa aacacgaaca agctctagca
     3841 acagatgcaa gatcatatat ttatttagtc caagataatg agattgataa agttaaaaaa
     3901 attgtaaaac agcaaaatat agatttattg ataactaagc atgataaaaa ggtgtttagt
     3961 agtggcaaac tgaaagctat cccaaatagt gatagctata ttcaaaaaag ggacgttttt
     4021 atttttaata agaaggttaa cggttatcat gtatggatta aaggttacaa taacgatatt
     4081 acagaaatgc attggacgtt gtggaaatat cttattttga catgtttagt tgtactcatt
     4141 tgtctgtatt ttgcaagccg tagttttaag cgtacattaa tccgtcccat tcaagaagtt
     4201 acttatgcaa cacaattgtt ggcaaatggt tattatcaca tacgtgtacc tgaaagtaat
     4261 gttgtagaaa ctaaagcgct ttttgtgtct accaatgatt tagcacgacg ccttcaaaag
     4321 cttaataatg agcaaaaaat tcaatccaac cgtttaaaaa caacgattga aaatattcca
     4381 agtgctattt taatgataga tcgaaatgga aaaatcgttg ttgcaaataa agcgtattat
     4441 gaacagttca acatctcgca taatattgaa caagtaggct atcacggcta tgttaatact
     4501 gaaattgagc aattaatttt agaaagtttt aaagttgaaa aacctattta tgaacaattg
     4561 gaagttgcaa taaaccaagt ccatgccaag tactttgata tttcatgcgt tccgatttta
     4621 actaggtctc aaaaaagttt gcaagggatt ttagttgtga tgcatgatat aacaaatctg
     4681 aaacaacttg aaaatctaag acgtgaattt gtagcaaatg tttcacatga attaaaaaca
     4741 cctattactt caattaaggg gtttgctgaa acgcttattg atggtgcaaa aaatgatgcg
     4801 gaatcattag acatgttttt aaacataatt ttaaaagaat caaatcgaat agaatcttta
     4861 gtaacagatc tgttagattt atcgcatatt gagcaacata cagagttaga tacagactat
     4921 atgaatttat cagatttaac gcggcgtatt attgataata tgatgactca agccaatcaa
     4981 aaaaatattt ccattcatac tgatattgaa aaagatgtca ttgttaaagc acaagaaagt
     5041 aaaattgctc aagttatcac gaatttgtta acgaatgcaa ttaactattc ttatgaagat
     5101 ggagatatta acgttcgtgt gtatcgagat gactttcgtg tcattttcga agtacaagat
     5161 tttggtatag gtattaaatt agaagaccaa caacgtattt ttgaaagatt ttatagagta
     5221 gataaagcac gaagtagaga ttctggtggg acaggtcttg gattatcaat tacaaagcat
     5281 atagtagagg cccaccaagg caatattgaa gtgaatagtc aagttggcaa aggctcaacg
     5341 ttcaaagtta ttctaaaaga ttataaagaa taaa
//
LOCUS       DAKTIO010000168         5236 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.170, whole genome shotgun sequence.
ACCESSION   DAKTIO010000168 DAKTIO010000000
VERSION     DAKTIO010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.170"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            165..881
                     /locus_tag="O0293_002324"
     CDS             165..881
                     /locus_tag="O0293_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCY0281195.1"
                     /translation="MGRKWNNIKEKKAQKDKNTSRIYAKFGKEIYVAAKSGEPNPESN
                     QALRLVLERAKTYSVPNHIIEKAIDKAKGAGDENFDHLRYEGFGPSGSMLIVDALTNN
                     VNRTASDVRAAFGKNGGNMGVSGSVAYMFDHVATFGIEGKSVDEILETLMEQDVDVND
                     VIDDNGLTIVYAEPDQFAVVQDALRAAGVEEFKVAEFEMLPQTDIELSEADQVTFEKL
                     IDALEDLEDVQNVFHNVDLK"
     gene            881..1354
                     /locus_tag="O0293_002325"
     CDS             881..1354
                     /locus_tag="O0293_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCY0281196.1"
                     /translation="MKSAEQWIDELQLESHPEGGFYRETIREVLKDGRRAPFSSIYFL
                     LTDDNISHFHRIDADEVWYYHAGDSLTIHMINPDGEYTTATLGTDIQNGDVLQYVVPK
                     GTIFASSIEISNTFSLVGCMCQPAFEFKQFELFKQSELITQYPHLKSVIEKYALK"
     gene            1446..1562
                     /locus_tag="O0293_002326"
     CDS             1446..1562
                     /locus_tag="O0293_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281197.1"
                     /translation="MLQLFLMKIALNDFENDKSVLFIFLKNHFYEEMCVLSN"
     gene            1707..2351
                     /locus_tag="O0293_002327"
     CDS             1707..2351
                     /locus_tag="O0293_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCY0281198.1"
                     /translation="MKVKYIDKRHWRRLIDREYTEVKVNNNRFKGIIGLVTMKKVRDP
                     LEVTVVGQNIIVADDNYKWLQILPEKKRYSITVMFDNKGNPLEYYFDINIKNITQKGN
                     ARTVDLCLDVLALPSGEYELVDEDDLMFALESEQITKKQFHEAYMIAHQIMAELENDF
                     KGFQKKIMYCFNKINAKAQKNHQKPQNKTNIEKSKQIKPKQYNQTKNHQQQKKN"
     gene            2467..3333
                     /locus_tag="O0293_002328"
     CDS             2467..3333
                     /locus_tag="O0293_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0281199.1"
                     /translation="MKIEDYRLLITLDETKTLRKAAEILYISQPAVTQRLKAIENAFG
                     VDIFIRTKKQLITTTEGTMIIEHARDMLKRERLFFDKMQAHIGEVNGTISIGCSSLIG
                     QTLLPEVLSLYNAQFPNVEIQVQVGSTEQIKANHRDYHVMITRGNKVMNLANTHLFND
                     DHYFIFPKNRRDDVTKLPFIEFQADPIYINQIKEWYNDNLEQDYHATITVDQVATCKE
                     MLISGVGVTILPEIMMKNISKEQFEFEKVEIDNEPLIRSTFMSYDPSMLQLPQVDSFV
                     NLMASFVEQPKA"
     gene            3603..4823
                     /locus_tag="O0293_002329"
     CDS             3603..4823
                     /locus_tag="O0293_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HCY0281200.1"
                     /translation="MDMFAALLQIKNYKLFVANMFLLGMGIAVTVPYLVLFATKDLGM
                     TTNQYGLLLASAAISQFTVNSIIARFSDTHHFNRKIIIILALLMGALGFSIYFFVDTI
                     WLFILLYAIFQGLFAPAMPQLYASARESINVSSSKDRAQFANTVLRSMFSLGFLFGPF
                     IGAQLIGLKGYAGLFGGTISIILFTLVLQVFFYKDLNIKHPISTQQHVEKIAPNMFKD
                     KTLLLPFIAFILLHIGQWMYTMNMPLFVTDYLKENEQHVGYLASLCAGLEVPFMIILG
                     VLSSRLQTRTLLIYGAIFGGLFYFSIGVFKNFYMMLAGQVFLAIFLAVLLGIGISYFQ
                     DILPDFPGYASTLFSNAMVIGQLGGNLLGGAMSHWVGLENVFFVSAASIMLGMILIFF
                     TKNQKITKEDVIST"
     gene            4820..>5236
                     /locus_tag="O0293_002330"
     CDS             4820..>5236
                     /locus_tag="O0293_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HCY0281201.1"
                     /translation="MTIILWLLIIAAFMLAFVGLIKPIIPSVLVLWVGFLIYQFGFHN
                     QHLSWVFYVSMALLTILILCADFLANKYFVNRFGGSKFGEYAALIGVVIGCFVLPPFG
                     IIIIPFILVFIVELIQGYSFERAVKVSIGSIVAFLTS"
BASE COUNT         1825 a          694 c          975 g         1742 t
ORIGIN      
        1 aatttgctta tcggcacatt tttaatttat acatatttta aaactaagta acagtttgaa
       61 gaaatcgtag ttcaataatg ttaattgtga aaatgtatat aaacataaaa aaatcatgta
      121 taatatatgt tgttaattaa acagttcgaa agcgagatga cattatggga cgtaaatgga
      181 ataacattaa agaaaaaaag gcccaaaaag ataaaaacac aagtagaata tatgcgaaat
      241 ttggtaagga gatttatgtt gcagcaaaat ctggtgaacc caatccagaa tctaaccaag
      301 ctttaaggtt ggtgcttgaa cgcgctaaga catattcagt gccgaatcat attattgaaa
      361 aagcaataga taaagctaag ggtgctggag acgaaaactt tgatcaccta agatatgaag
      421 gatttggccc aagcggatca atgctaattg ttgatgcgtt aacaaataat gtaaatcgta
      481 ctgcctctga tgtgcgagct gcttttggta aaaacggcgg taatatgggt gtatctggat
      541 cagttgctta tatgtttgat catgtggcaa catttggtat tgaaggaaag tctgttgacg
      601 aaatacttga aacattaatg gaacaagatg tagatgtaaa tgatgtgatt gacgataatg
      661 gattgacaat agtctatgct gaaccagatc aatttgcagt cgttcaagat gcgcttcgtg
      721 cagcaggtgt tgaagaattt aaagttgctg aatttgaaat gttacctcaa acagatattg
      781 aactttctga agcggaccaa gtaacatttg aaaaattaat cgatgcatta gaagatttag
      841 aagatgtaca aaacgtattc cataatgtgg atttgaaata atgaaatcag cagaacaatg
      901 gattgatgaa ttgcaacttg aatcacatcc tgaaggtggt ttctatagag agacaattcg
      961 agaagtattg aaagatggac gcagagcgcc gtttagtagt atttattttt tacttacaga
     1021 tgacaatatt tcgcattttc atcgaattga tgctgatgaa gtatggtact atcatgctgg
     1081 cgattctcta acaattcata tgataaatcc ggatggggaa tatacgactg caacattggg
     1141 tactgatatc caaaatggag atgtattgca atatgtagtg cctaaaggaa caatttttgc
     1201 ttcttcaatc gaaatatcaa atacttttag tttagtaggt tgtatgtgtc aaccggcatt
     1261 tgagtttaag cagtttgaat tgtttaagca atctgaatta attacgcaat atccgcatct
     1321 taaatcagta atcgaaaaat atgctttaaa ataaaagtga tcaatgaagt ggtttgaagg
     1381 ttgttaataa acctttgagt cacttcattt ttatatgtat tcttgattga atcagaatag
     1441 atttgatgct tcagctgttt ttaatgaaaa tagcattaaa tgattttgaa aacgataaga
     1501 gtgtgttatt tatatttttg aaaaatcact tttatgaaga aatgtgtgta ctatctaatt
     1561 aatattgtct attttaagta aattgaagcg agttgtagta aaataataat aaatattttc
     1621 atatgattaa caaaaactat aaaactgtat catgacatga catcatttat gtagttttat
     1681 ataataatga caaggagttg gtgactgtga aagtaaagta tatagataaa cgtcactggc
     1741 gtcgcctaat tgatagggaa tacacagagg taaaagttaa taataatagg tttaagggta
     1801 ttataggctt agtcacgatg aaaaaggttc gtgatccttt agaggtgacg gtagttggac
     1861 aaaatatcat tgtcgcagat gacaattata aatggttgca aatactacct gaaaagaaac
     1921 gttatagtat aactgtaatg tttgataata aaggcaatcc attagaatat tattttgata
     1981 taaatatcaa aaatataacg caaaaaggta atgcgcgtac agtagattta tgtttagatg
     2041 ttttagcatt accaagtggt gaatatgagt tggtagatga agatgattta atgtttgcat
     2101 tagaaagtga gcaaattaca aaaaagcaat ttcatgaagc atatatgatt gcacatcaaa
     2161 ttatggcaga gttagaaaat gattttaaag gattccaaaa gaaaatcatg tactgcttta
     2221 ataaaattaa tgcaaaggct caaaaaaatc atcaaaagcc acaaaataaa actaatattg
     2281 aaaaaagcaa acaaataaag cctaagcaat ataatcaaac taaaaatcac caacaacaaa
     2341 agaaaaacta agtaattcaa gctgcagcca taccaataaa attggtgaaa ttattcgtta
     2401 gtaatgaact catgaatgct tgtagccatg aaagttcaat aattgaataa tttatggggg
     2461 gaaattatga agattgaaga ctatcgttta ctaataacat tagacgaaac gaaaacgtta
     2521 cgtaaagcgg ctgaaatttt atatatatct caacctgctg ttacacaaag actaaaagct
     2581 attgaaaatg cttttggagt agatattttt atcagaacaa aaaaacaatt gattacaaca
     2641 actgaaggaa caatgattat tgagcatgct cgtgacatgt tgaaaagaga gcgattattt
     2701 tttgacaaaa tgcaggcaca tattggtgaa gtgaatggaa caatatcaat cgggtgttct
     2761 tctttgattg gacaaacctt acttcctgaa gttttgagcc tatataatgc ccaatttcct
     2821 aatgttgaaa tacaagtgca agttggttca actgaacaaa ttaaagcaaa tcatagagat
     2881 tatcatgtta tgataactcg tggaaataag gtaatgaatt tagctaacac acatttattt
     2941 aatgatgatc attattttat ttttccaaaa aatagacgag atgatgttac aaagttacca
     3001 tttatagagt ttcaagctga tccgatttat ataaatcaaa taaaagaatg gtataacgat
     3061 aatttagaac aagattacca tgcaactatt acagtggatc aagtagcaac ttgcaaagaa
     3121 atgttgatta gtggtgtagg tgttacaatc ttgccagaaa ttatgatgaa aaatatcagc
     3181 aaagaacaat ttgagtttga aaaagtagaa attgataatg aaccgctgat tcgttcgaca
     3241 tttatgagtt atgatccgag catgttgcaa ttgccacaag ttgattcttt tgtaaatctc
     3301 atggcgagct ttgttgaaca accaaaggcg tagttttaga ctaatttaag gtttgtattt
     3361 aattttaaac tattcggtta aattgaacgt agttggttgc taatgcacca acagcaaaag
     3421 agcccctaat taataaatta aaaggggaca aaggaataca gttggttgct aatgcaccaa
     3481 ctgcataaga gcccctaatt aataaattaa aaggggacaa aggaatacag ttggatgcta
     3541 acgcaccaac tgcataagag cccctaatta ataaattaaa aggggctcta agaagcgggg
     3601 ttatggatat gtttgcagcg ttattacaaa taaagaatta taaactcttt gttgctaata
     3661 tgtttctact aggtatgggt attgcggtta cggtcccata tcttgttctt tttgcaacta
     3721 aagatttagg tatgacaaca aatcagtatg gattacttct agcatctgca gcgattagcc
     3781 agtttacagt aaattcaatt attgctagat tttcggatac gcatcacttt aatagaaaaa
     3841 ttattattat tctcgcatta ttaatgggtg cgcttggttt ttcaatatac ttttttgtag
     3901 atacaatctg gttattcata ttactatatg cgattttcca aggattattt gcaccagcaa
     3961 tgccccaact ttacgcatct gctagagaat ctatcaatgt ttcaagctct aaagatagag
     4021 ctcaatttgc caacacagta ttacgttcaa tgttctcatt gggcttttta tttggtccat
     4081 ttattggtgc ccaattaatc ggattaaaag gctatgctgg attgtttggt ggaacaataa
     4141 gtatcatttt atttacttta gtacttcaag tgtttttcta taaggattta aacattaaac
     4201 accctattag tacgcaacaa catgttgaaa aaattgctcc taatatgttt aaagacaaaa
     4261 cgcttttatt accatttatt gcatttattt tattacacat tggacaatgg atgtatacga
     4321 tgaatatgcc tttatttgtt actgattatt taaaagaaaa tgaacaacat gtcggttatt
     4381 tagctagttt atgtgctggt ttagaagtgc catttatgat cattcttggc gttttatcat
     4441 ctagattaca gactcgaaca ttgttgattt atggagcgat ttttggtggt ttattctact
     4501 tcagcattgg ggtatttaaa aacttctata tgatgttagc aggacaggtg tttttagcta
     4561 ttttcttagc ggttctttta ggaattggta ttagttattt ccaagatatc ttaccagatt
     4621 ttccaggata cgcttcaaca ctattttcta atgcaatggt tattggacag ttaggcggta
     4681 acctattagg tggtgctatg agtcactggg taggtttgga aaatgtattt tttgtatcag
     4741 cagcatcaat catgttaggt atgatactta tattctttac taaaaatcaa aaaattacaa
     4801 aagaggatgt gatatcaaca tgacaattat tttatggcta cttatcatcg ctgccttcat
     4861 gttagcattt gttgggttga ttaagccgat tattccttct gttttagtat tatgggttgg
     4921 ctttttaatc tatcaatttg gctttcataa tcagcattta tcatgggtgt tttatgtatc
     4981 tatggcattg ctaacaatat taattttatg tgccgacttt ttagctaata aatattttgt
     5041 gaatcgcttc ggtggttcta agtttggaga gtatgcagct ttaattggtg tggttattgg
     5101 atgttttgtt ttaccgccat ttggaattat tattatacct tttattttgg tattcatagt
     5161 tgaattaata caaggctatt catttgaaag agcagttaaa gtaagtatag gttcaatcgt
     5221 agcattttta acaagt
//
LOCUS       DAKTIO010000169         5198 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.171, whole genome shotgun sequence.
ACCESSION   DAKTIO010000169 DAKTIO010000000
VERSION     DAKTIO010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.171"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(35..1573)
                     /gene="putP"
                     /locus_tag="O0293_002331"
     CDS             complement(35..1573)
                     /gene="putP"
                     /locus_tag="O0293_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCY0281202.1"
                     /translation="MLTMGTALSQQVDANWQTYIMIAVYFLILIVIGFYGYKQATGNL
                     SEYMLGGRSIGPYITALSAGASDMSGWMIMGLPGSVYSTGLSAMWITIGLTLGAYINY
                     FVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSG
                     GKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAM
                     MNLNGWGTFHDVAAMKPTNLNLFKGLSFIGIISLFSWGLGYFGQPHIIIRFMSIKSHK
                     MLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAYHIKLEDPETLFIVMSQVLFHPLVG
                     GFLLAAILAAIMSTISSQLLVTSSSLTEDFYKLIRGEEKAKTHQKEFVMIGRLSVLVV
                     AIVAIAIAWNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGMVSGALV
                     VIVWIAWIKPLAHINEIFGLYEIIPGFIVSVIVTYVVSKLTKKPGAFVETDLNKVRDI
                     VREK"
     gene            1940..2242
                     /gene="gatC"
                     /locus_tag="O0293_002332"
     CDS             1940..2242
                     /gene="gatC"
                     /locus_tag="O0293_002332"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HCY0281203.1"
                     /translation="MTKVTREEVEHIANLARLQISPEETEEMANTLESILDFAKQNDS
                     ADTEGVEPTYHVLDLQNVLREDKAIKGIPQELALKNAKETEDGQFKVPTIMNEEDA"
     gene            2244..3701
                     /gene="gatA"
                     /locus_tag="O0293_002333"
     CDS             2244..3701
                     /gene="gatA"
                     /locus_tag="O0293_002333"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HCY0281204.1"
                     /translation="MSIRYESVENLLTLIKDKKIKPSDVVKDIYDAIEETDPTIKSFL
                     ALDKENAIKKAQELDELQAKDQMDGKLFGIPMGIKDNIITNGLETTCASKMLEGFVPI
                     YESTVMEKLHNENAVLIGKLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAA
                     AVAAGLVPFSLGSDTGGSIRQPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTR
                     NVKDNAIVLEAISGADVNDSTSAPVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDV
                     KEAVQNAVETLKSLGAVVEEVSLPNTKFGIPSYYVIASSEASSNLSRFDGIRYGYHSK
                     EAHSLEELYKMSRSEGFGKEVKRRIFLGTFALSSGYYDAYYKKSQKVRTLIKNDFDKV
                     FENYDVVVGPTAPTTAFNLGEEIDDPLTMYANDLLTTPVNLAGLPGISVPCGQSNGRP
                     IGLQFIGKPFDEKTLYRVAYQYETQYNLHDVYEKL"
     gene            3714..5141
                     /gene="gatB"
                     /locus_tag="O0293_002334"
     CDS             3714..5141
                     /gene="gatB"
                     /locus_tag="O0293_002334"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HCY0281205.1"
                     /translation="MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAY
                     PGVLPVVNKRAVDWAMRAAMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGEN
                     GYIDIEVDGETKRIGITRLHMEEDAGKSTHKGEYSLVDLNRQGTPLIEIVSEPDIRSP
                     KEAYAYLEKLRSIIQYTGVSDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFN
                     YVRKGLEYEEKRQEEELLNGGEIGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIV
                     PLYIDDAWKERVRQTIPELPDERKAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHG
                     ADVKLTSNWLMGGVNEYLNKNQVELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPE
                     LAAKGGNAKQIMEDNGLVQISDEATLLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQ
                     IMKASKGQANPQLVNQLLKQELDKR"
BASE COUNT         1911 a          844 c          918 g         1525 t
ORIGIN      
        1 attagtacct ttttaatttg ttaagactta tgaattattt ttctctaacg atgtcacgaa
       61 ctttgtttaa gtcagtttca acaaatgcac caggtttttt agtaagttta cttacaacat
      121 atgtaacgat tacacttaca ataaatccag gaataatttc atataagccg aatatttcgt
      181 tgatatgtgc caatggttta atccacgcaa tccaaacgat aacgactaag gcacctgaaa
      241 ccattccact tacagcaccg gcacgtgtca aacctttcca gtaaagtgca aatagcacaa
      301 gtggactgaa cgatgcacca aatccggccc aagcgttacc tactaagttt agaattgtgt
      361 cgtttggatt ccatgcaatc gcgatggcaa caattgctac aactaataca gataatcttc
      421 caatcataac aaattctttt tggtgcgttt tagctttttc ttcaccacga attaatttat
      481 aaaagtcttc cgttagtgaa ctagatgtta caagtaattg tgaagaaatc gtgctcataa
      541 ttgcagctag aatcgcagca agtaagaaac cacctacaag aggatggaag agtacttgac
      601 tcatcacgat gaataatgtc tcaggatctt ctagtttaat atgataagca ggtacgaatg
      661 caatacctgt taaaccaaca gccacagcgc ctaataaacc aacagccatc cagctaatac
      721 ctaaacgtct agctttaggt agcatcttgt gtgatttaat agacataaac cttataatga
      781 tatgaggttg accgaaataa cctaatcccc atgaaaatag agagataatt cctataaatg
      841 ataacccttt aaataaattt aaatttgtag gtttcatagc tgctacatca tgaaacgttc
      901 cccagccgtt taaattcatc atagcaacaa tagggaccat aaccatcgca attaacataa
      961 tgacaccttg gaagaaatct gtaattgata cagctaaata tccaccaaag aaagtataga
     1021 aaatgacaat gaaagcaact aatattaaac cgaaatgata atctaatcca aaagcacttt
     1081 caaatagttt accaccagat acgaaaccag aatgtgtata taatgtaaag aatactacga
     1141 taatcaatcc agaaataatc tttaacacat tatttttatc gtttaaacga tttttaaaga
     1201 aatctggtaa tgtaattgca tctccagcta attcggtata aacacgaagt ctaggagcaa
     1261 caacaaagta atttatataa gcacctaatg ttaaaccgat tgtaatccac atagctgata
     1321 gaccagtgct atagacagaa ccaggtagcc ccataatcat ccatccactc atatctgaag
     1381 ctccagctga taatgcagta atatacggtc caatactacg tccacctaac atgtactcgc
     1441 ttaggttacc agttgcttgc ttgtaaccgt aaaagccaat aacgattagt atcaagaagt
     1501 agacggcaat cataatataa gtttgccaat tggcatctac ttgttgactt aatgctgtcc
     1561 ccattgtaag catgataaac actcccttta tttaatttat aatttagtta cttgaaaaca
     1621 ttatacataa agataaatgt ttagaaaagt aggaaaatta aatttattct ttttgaaata
     1681 cttgcatacc aatatagaaa gcgctttcgt attttttgga tatgagcaaa tttttataat
     1741 taaaattgca taatcataat attgaattgg gttgtctaat attaaaagag tgttgttttc
     1801 ataaaaaatg atttgataaa cgaatgataa tgtttataca gtttatatga aattaaagta
     1861 gcacctcata aatacttaga tttttaattg gaaatttgat acaatttagt gatgaatgac
     1921 ttaaaggagg cttttattaa tgacaaaagt aacacgtgaa gaagttgagc atatcgcgaa
     1981 tcttgcaaga cttcaaattt ctcctgaaga aacggaagaa atggccaaca cattagaaag
     2041 cattttagat tttgcaaaac aaaatgatag cgctgataca gaaggcgttg aacctacata
     2101 tcacgtttta gatttacaaa acgttttacg tgaagataaa gcaattaaag gtattccaca
     2161 agaattagct ttgaaaaatg ccaaagaaac agaagatgga caatttaaag tgcctacaat
     2221 catgaatgag gaggacgcgt aagatgagca ttcgctacga atcggttgag aatttattaa
     2281 ctttaataaa agacaaaaaa atcaaaccat ctgatgttgt taaagatata tatgatgcaa
     2341 ttgaagagac tgatccaaca attaagtctt ttctagcgct ggataaagaa aatgcaatca
     2401 aaaaagcgca agaattggat gaattacaag caaaagatca aatggatggc aaattatttg
     2461 gtattccaat gggtataaaa gataacatta ttacaaacgg attagaaaca acatgtgcaa
     2521 gtaaaatgtt agaaggtttt gtgccaattt acgaatctac tgtaatggaa aaactacata
     2581 atgaaaatgc cgttttaatc ggtaaattaa atatggatga gtttgcaatg ggtggttcaa
     2641 cagaaacatc ttatttcaaa aaaacagtta acccatttga ccataaagca gtgccaggtg
     2701 gttcatcagg tggatctgca gcagcagttg cagctggctt agtaccattt agcttaggtt
     2761 cagacacagg tggttcaatt agacaaccgg ctgcatattg tggcgttgtc ggtatgaaac
     2821 caacatacgg tcgtgtatct cgatttggat tagttgcttt tgcatcttca ttagaccaaa
     2881 ttggtccatt gactcgaaat gtaaaagata atgcaatcgt attagaagct atttctggtg
     2941 cagatgttaa tgactctaca agtgcaccag ttgatgatgt agactttaca tctgaaattg
     3001 gtaaagatat taaaggatta aaagttgcat tacctaaaga atacttaggt gaaggtgtag
     3061 ctgatgacgt aaaagaagca gttcaaaacg ctgtagaaac tttaaaatct ttaggtgctg
     3121 tcgttgagga agtatcattg ccaaatacta aatttggtat tccatcatat tacgtgattg
     3181 catcatcaga agcttcgtca aacctttctc gttttgacgg aattcgttat ggttatcatt
     3241 ctaaagaagc tcattcatta gaagaattat ataaaatgtc aagatctgaa ggtttcggta
     3301 aagaagtaaa acgtcgtatt ttcttaggta catttgcatt aagttcaggt tactatgatg
     3361 cttactataa aaaatctcaa aaagttagaa cattgattaa aaatgacttt gataaagtat
     3421 tcgaaaatta tgatgtagta gttggtccaa cagcgcctac aactgcgttt aatttaggtg
     3481 aagaaattga tgatccatta acaatgtatg ccaatgattt attaacaaca ccagtaaact
     3541 tagctggatt acctggtatt tctgttcctt gtggacaatc aaatggccga ccaatcggtt
     3601 tacagttcat tggtaaacca ttcgatgaaa aaacgttata tcgtgtcgct tatcaatatg
     3661 aaacacaata caatttacat gacgtttatg aaaaattata aggagtggaa atcatgcatt
     3721 ttgaaacagt tataggactt gaagttcacg tagagttaaa aacggactca aaaatgtttt
     3781 ctccatcacc agcgcatttt ggagcagaac ctaactcaaa tacaaatgtt atcgacttag
     3841 catatccagg tgtcttacca gttgttaata agcgtgcagt agactgggca atgcgtgctg
     3901 caatggcact aaatatggaa atcgcaacag aatctaagtt tgaccgtaag aactatttct
     3961 atccagataa tccaaaagca tatcaaattt ctcaatttga tcaaccaatt ggtgaaaatg
     4021 gatatatcga tatcgaagtc gacggtgaaa caaaacgaat cggtattact cgtcttcaca
     4081 tggaagaaga tgctggtaag tcaacacata aaggtgagta ttcattagtt gacttgaacc
     4141 gtcaaggtac accgctaatt gaaatcgtat ctgaaccaga tattcgttca cctaaagaag
     4201 catatgcata tttagaaaaa ttgcgttcaa ttattcaata cactggtgta tcagacgtta
     4261 agatggaaga gggatcttta cgttgtgatg ctaacatctc tttacgtcca tatggtcaag
     4321 aaaaatttgg tactaaagcc gaattgaaaa acttaaactc atttaactat gtacgtaaag
     4381 gtttagaata tgaagaaaaa cgccaagaag aagaattgtt aaatggtgga gaaatcggac
     4441 aagaaacacg tcgatttgat gaatctacag gtaaaacaat tttaatgcgt gttaaagaag
     4501 gttctgatga ttaccgttac ttcccagagc ctgacattgt acctttatat attgatgatg
     4561 cttggaaaga gcgtgttcgt cagacaattc ctgaattacc agatgaacgt aaagctaagt
     4621 atgtaaatga attaggttta cctgcatacg atgcacacgt attaacattg actaaagaaa
     4681 tgtcagattt ctttgaatca acaattgaac acggtgcaga tgttaaatta acatctaact
     4741 ggttaatggg tggcgtaaac gaatatttaa ataaaaatca agtagaatta ttagatacta
     4801 aattaacacc agaaaattta gcaggtatga ttaaacttat cgaagacgga acaatgagca
     4861 gtaaaattgc gaagaaagtc ttcccagagt tagcagctaa aggtggtaat gctaaacaga
     4921 ttatggaaga taatggctta gttcaaattt ctgatgaagc aacacttcta aaatttgtaa
     4981 atgaagcatt agacaataac gaacaatcag ttgaagatta caaaaatggt aaaggcaaag
     5041 ctatgggctt cttagttggt caaattatga aagcgtctaa aggtcaagct aatccacaat
     5101 tagtaaatca actattaaaa caagaattag ataaaagata atttaaatca tcaaactatg
     5161 aagatttaaa aaataaaccc ttgattgctg acttagat
//
LOCUS       DAKTIO010000170         5196 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.172, whole genome shotgun sequence.
ACCESSION   DAKTIO010000170 DAKTIO010000000
VERSION     DAKTIO010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.172"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(127..405)
                     /gene="qoxD"
                     /locus_tag="O0293_002335"
     CDS             complement(127..405)
                     /gene="qoxD"
                     /locus_tag="O0293_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit IV"
                     /protein_id="HCY0281206.1"
                     /translation="MKHTVGFIASIVLTLLAVYVTLYTSLTFHAKLTIIFGFAFVQAG
                     LQLLMFMHLTEGKDGRLQTFKVIFALVITLCFVVGTYWVMQGGHSSHL"
     gene            complement(414..1019)
                     /gene="qoxC"
                     /locus_tag="O0293_002336"
     CDS             complement(414..1019)
                     /gene="qoxC"
                     /locus_tag="O0293_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit III"
                     /protein_id="HCY0281207.1"
                     /translation="MSHDTNTIDSRTHEGELNKLGFWIFITAEFALFGTLFATLLTLQ
                     HGGDYAGKMTTELFELPLVLIMTFALLFSSYTCGIAIYYMRQEKQKLMMFWMIITLLL
                     GLVFVGFEIYEFAHYASEGVNPTIGSYWSSFFILLGTHGCHVSLGIVWAICLLIQIQR
                     RGLDKYNAPKLFIVSLYWHFLDVVWVFIFTAVYMIGMVYSG"
     gene            complement(1009..2997)
                     /gene="qoxB"
                     /locus_tag="O0293_002337"
     CDS             complement(1009..2997)
                     /gene="qoxB"
                     /locus_tag="O0293_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit I"
                     /protein_id="HCY0281208.1"
                     /translation="MNFPWDQLLVKGNWMITMAQIGAPFLVIGLIAVITYFKLWKYLY
                     KEWFTSVDHKKIGIMYLICAVLMFVRGGIDALLIRAQLTVPDNKFLESNHYNEIFSTH
                     GVIMIIFMAMPFIFGLWNIVVPLQIGARDVAFPVLNNVSFWLFFAGMILFNLSFIIGG
                     SPAAGWTNYAPLAGEFSPGPGVNYYLIAIQISGLGTLATGINFFVTILRCKTPTMKFM
                     QMPMFTVTTFITTLIVILAFPPLTVALALMTTDRIFDTAFFTVAHGGMPMLWANFFWV
                     WGHPEVYIVILPAFGIYSEIIPTFARKRLFGHQSMVWATAGIAFLSFLVWVHHFFTMG
                     NGALINSFFSISTMLIGIPTGVKLFNWLLTLYKGRITFESPMLFSLAFIPNFLLGGVT
                     GVMLAMASADYQYHNTYFLVAHFHYTLVTGVVFACLAGLIFWYPKMMGYKLNETLNKW
                     CFWFFMIGFNVCFLPQFILGLDGMPRRLYTYMPSDGWFLLNLISTIGALLMAIGFLFL
                     VVSIVYSHFKSPREATGDNWDGLGRTLEWTTASAIPPKYNFAITPDWNDYDTFVDMKE
                     HGRHYLDNHNYKDIHMPNNTPVGFWIGIFMTIGGFFLIFETVIPALICLFGIFGTMIY
                     RSFQIDHGYHIPAAEVAETEARLREARIKEREAVSHES"
     gene            complement(2997..4097)
                     /gene="qoxA"
                     /locus_tag="O0293_002338"
     CDS             complement(2997..4097)
                     /gene="qoxA"
                     /locus_tag="O0293_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit II"
                     /protein_id="HCY0281209.1"
                     /translation="MSKFKSLLLLFGTLILLSGCSNIEIFNAKGPVASSQKFLILYSI
                     VFMLVICFVVLGMFAIFIYKYSYNKNAESGKMHHNAIIETIWFVIPIIIVAALAIPTV
                     KTLYDYEKPPKSEKDPMVVYAVSAGYKWFFAYPDEHIETVNTLTIPKDRPVVFKLQAM
                     DTMTSFWIPQLGGQKYAMTGMTMNWTLEASQTGTFRGRNSNFNGEGFSRQTFKVNAVS
                     QKDYDKWVKEVKGKKTLDQDTFDKQLLPSTPNKALEFNGTHMAFVDPAADPEYIFYAY
                     KRFNFELKDPNFTSEENMFKDVSDKPLIPARKAQITNANYKRHGMKLMILGNDEPYNN
                     EFKKDESKNAKEMKKISKDAQDQDNDDHGGGH"
     gene            4399..4521
                     /locus_tag="O0293_002339"
     CDS             4399..4521
                     /locus_tag="O0293_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281210.1"
                     /translation="MIERKRKHFNKEFAKSNLSLNNEKWKFVCSKSKHFFKKSS"
     gene            complement(4669..4986)
                     /locus_tag="O0293_002340"
     CDS             complement(4669..4986)
                     /locus_tag="O0293_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HCY0281211.1"
                     /translation="MNKLLQSLSALGVSATLVTPNLNADATTNTTPQIKGANDIVIKK
                     GQDYNLLNGISAFDKEDGDLTDKIKVDGQIDTSKSGKYQIKYHVTDSDGAIKISTRYI
                     EVK"
BASE COUNT         1796 a          871 c          888 g         1641 t
ORIGIN      
        1 tgaattgatt ataatgattg tcttataaga tatagaaggg gacttaacca tttaaaaatt
       61 gaaggaaaat aaaaaccagc taacaaaagt gttagctggt taaactttac attattaaaa
      121 tgttgtttat aagtgtgaag agtgaccgcc ttgcataacc caatatgttc cgacaacgaa
      181 acaaagtgta attacaagag caaagataac tttgaatgtt tgtaaacgtc catctttacc
      241 ttcagttaaa tgcatgaaca ttaataattg aagtcctgct tggacgaatg caaagccaaa
      301 gataattgtc aacttcgcgt ggaatgttaa tgacgtgtat agtgttacgt aaactgctaa
      361 aagcgttaat acgatagatg cgataaatcc tacagtatgt ttcattattg tactcatccg
      421 ctatacacca tccctatcat atatacggca gtaaagatga aaacccaaac aacatctaag
      481 aagtgccagt ataaacttac tataaataat tttggcgcat tatatttgtc taatccgcgt
      541 cgttggattt ggattaataa acaaatggcc caaacgatac ctagcgatac gtgacaacca
      601 tgcgttccta ataggataaa gaaactagac cagtaagaac caattgttgg gttaacgcct
      661 tctgatgcat agtgtgcgaa ttcataaatt tcgaatccaa caaagactaa acctaaaagt
      721 aacgtaatga tcatccaaaa catcattaac ttttgttttt cttggcgcat gtagtaaata
      781 gcaataccac atgtgtaaga actgaataat aatgcaaacg tcattattaa aacaagaggc
      841 aattcaaata actcagtagt cattttacct gcataatcgc caccatgttg caaagttaat
      901 agtgtcgcaa atagggtacc gaataacgca aattcggctg taatgaaaat ccaaaagcca
      961 agcttattta attcgccttc atgtgtgcgt gaatcaatag tgtttgtatc atgactcatg
     1021 acttacagcc tccctttctt taatacgcgc ttctcttaat ctagcttcag tttctgcaac
     1081 ttcagcagca gggatatgat atccgtgatc gatttggaaa ctgcgataaa tcatagtacc
     1141 aaaaataccg aataaacaaa ttaatgctgg aattacagtt tcgaaaatta agaagaaacc
     1201 gccgatagtc ataaagatac caatccagaa tccaacagga gtattgtttg gcatatgaat
     1261 gtctttgtaa ttatggttgt ctaagtaatg acgaccatgt tctttcatat caacaaatgt
     1321 gtcgtagtca ttccaatctg gtgtaatggc aaagttgtat ttaggtggaa ttgctgatgc
     1381 agtagtccac tctagagtac gaccaaggcc atcccagtta tctccagttg cttcacgtgg
     1441 agatttgaag tgactgtata cgatactaac aacaaggaat aagaatccga ttgccattaa
     1501 taatgcaccg atagttgaaa ttaagtttaa taagaaccaa ccatctgatg gcatataagt
     1561 gtataaacga cgtggcatac catctaatcc aagaatgaat tgtggtaaga aacaaacgtt
     1621 aaatccgatc atgaagaacc agaagcacca tttgtttaat gtttcgttta atttgtaacc
     1681 catcatcttt ggataccaga agattaaacc agctaagcag gcaaatacaa caccagtaac
     1741 caatgtatag tggaagtgag ctactaagaa gtacgtattg tgatattgat agtcagctga
     1801 tgccattgct aacattacac ccgtaacacc acctaaaagg aagttaggga taaatgctaa
     1861 tgagaatagc attggtgact caaatgtaat acgtccttta tataatgtta ataaccagtt
     1921 aaacaatttc acaccagttg gaataccaat cagcattgtt gaaattgaga agaatgagtt
     1981 gattaacgca ccattaccca ttgtgaagaa atggtgaacc caaactaaga aactaaggaa
     2041 cgcgatgccg gcagttgccc ataccatact ttgatgtccg aataaacgct tacgagcgaa
     2101 tgtcgggata atttctgagt aaataccaaa tgctggaagg ataacgatat aaacttcagg
     2161 gtgcccccat acccagaaga agttagccca aagcattggc ataccgccat gtgcaactgt
     2221 gaagaatgct gtgtcaaata ttctatcagt tgtcattaat gctaacgcta ctgttaaagg
     2281 agggaaagca agaataacaa ttaatgtagt aataaatgtt gttactgtaa acattggcat
     2341 ttgcataaac ttcatagttg gtgttttaca tcttaaaatt gttacaaaga agttgatacc
     2401 tgtagctaag gtaccaagcc ctgaaatttg tatagctatt aagtaatagt taacacccgg
     2461 accaggactg aattcacctg ctagtggcgc atagtttgtc caaccagctg ctggtgaacc
     2521 accaataata aatgacaggt tgaataaaat catacctgca aagaatagcc agaaacttac
     2581 gttgtttaat actgggaatg caacatcacg tgctccaatt tgtaatggaa caacgatatt
     2641 ccataaacca aagataaatg gcattgccat gaagataatc atgattacac catgtgtact
     2701 aaaaatttcg ttatagtggt tagattctaa aaatttgtta tcaggtactg ttaattgcgc
     2761 acgaataagt aacgcatcaa taccaccacg gacgaacatt aatacggcac agattaaata
     2821 cataataccg attttcttat ggtctacaga tgtgaaccat tctttgtaaa gatatttcca
     2881 taatttaaag taagtaatta ctgcgattaa accaataact aagaatgggg caccaatttg
     2941 tgccattgta atcatccagt tacctttaac tagtaattga tcccatggaa aattcattaa
     3001 tgtccacctc catgatcatc attgtcttga tcttgcgcat cttttgaaat tttcttcatt
     3061 tctttcgcat ttttcgattc atctttcttg aactcattgt tatatggttc gtcatttcca
     3121 agaatcatca acttcatacc atgtcgttta tagttcgcat ttgtaatttg agctttacga
     3181 gcaggtatta atggtttgtc tgatacatct ttaaacatat tttcttcact agtgaagttt
     3241 ggatctttta attcgaaatt gaaacgttta tatgcataga agatgtattc tggatcggct
     3301 gctggatcaa caaacgccat atgtgtacca ttaaattcta aagctttatt aggtgtgctt
     3361 ggtaataatt gtttatcaaa tgtatcttga tctaacgttt tcttaccttt aacttctttc
     3421 acccatttgt cgtagtcttt ttgactaacg gcatttactt taaatgtttg acgtgagaat
     3481 ccttcaccat tgaagttaga gttacgacct ctgaacgtac cagtttgaga tgcttctaac
     3541 gtccaattca ttgtcatgcc agtcatggca tatttttgac cacctaattg tggaatccag
     3601 aaacttgtca ttgtatccat agcttgaagc ttaaatacaa caggacgatc tttagggatt
     3661 gttaatgtat taacagtctc tatatgttca tctggataag caaagaacca tttgtatcct
     3721 gcacttactg catatacaac cattggatct ttctcactct tcggtggttt ttcgtaatcg
     3781 tataaagttt taactgtagg aatagctaaa gcagcaacga ttatgatagg tattacaaac
     3841 catattgttt caatgatggc attatggtgc atcttaccag attcggcatt cttattataa
     3901 ctatacttgt aaataaaaat ggcgaacatg ccaagtacaa cgaaacaaat aacaagcatg
     3961 aagacgattg aataaagaat caagaacttc tgactacttg ctactggccc ttttgcgttg
     4021 aaaatttcta tatttgaaca accactaagt aaaattagtg tgccaaataa tagaagcaaa
     4081 gacttaaatt ttgacacttt tttgacctcc taatactaca aatgtagggc ttaacattaa
     4141 ttttaagtta ttacacaata tttacaaggg cttatgggaa aaaaattaat aaaattgtat
     4201 caaaaatgtt gataaatcaa ggtgtgacgt gggttcacac atttgttaaa attatgtgta
     4261 cattttgtga ctaatagcgt ttttgaaaaa attgcttcat atttaaaagg aaagttgaga
     4321 atagctttca attaaaggtt aattgaatga aaataaaaaa agtttgaaat tgtctaataa
     4381 attgaaaatg atggctagtt gattgaaagg aagcgcaaac attttaataa ggaatttgcc
     4441 aaaagtaatt tatcgttaaa caatgaaaag tggaaatttg tttgttcaaa atcaaaacat
     4501 ttttttaaaa agagcagtta agttttaata atttgatagg tagtcctttg gtatacagaa
     4561 aaaatacaat atttgggtta aagttgcaga tgaagttaga ataacaaata aaatggtagc
     4621 aaaacatgtt cgtttgctac cattttattt gcagtatagt gatgagggct atttaacctc
     4681 aatataccta gtggaaattt taattgcacc atctgaatca gtgacatgat atttaatttg
     4741 atatttacca gatttagatg tatcaatttg gccatcgact ttaattttat cggttaaatc
     4801 tccatcttct ttatcaaatg cacttatgcc gtttagaagg ttataatctt gacctttctt
     4861 aataacgata tcattagcgc ctttaatttg tggtgtagta ttcgtcgttg catctgcatt
     4921 taaatttggt gttactagtg tagcagaaac accgagggct gataatgact gtaatagttt
     4981 attcataatg taacctccga atgataagtc atttctgagt tagctttact ataccttaat
     5041 ttatattatt tacacatcat actatagtcc taatcttacc ctataaagaa tgaaatttaa
     5101 atataaattt ataaataatg aggaaataat tattattttg aaaatctgcc acaattgtaa
     5161 aattaataat ttagggataa tttgaaatca aatata
//
LOCUS       DAKTIO010000171         5124 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.173, whole genome shotgun sequence.
ACCESSION   DAKTIO010000171 DAKTIO010000000
VERSION     DAKTIO010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.173"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            124..1761
                     /locus_tag="O0293_002341"
     CDS             124..1761
                     /locus_tag="O0293_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCY0281212.1"
                     /translation="MNKDKQLHNDKINLSQLVLLGLGSLIGSGWLFGAWEASSIAGPA
                     AIISWVLGFLVIGTIAYNYIEIGTMFPQSGGMSNYAQYTHGSLLGFIAAWANWVSLVT
                     IIPIEAVSAVQYMSSWPWHWAKPMRYLMENGSISTYGLLAVYLIIVIFSLLNYWSVKL
                     LTSFTSLISVFKLGVPMLTIIMLMLSGFDTSNYGHSASTFMPYGSAPIFAATTASGII
                     FSFNSFQTIINMGSEIKNPEKNIARGIAISLSISAVLYIILQSTFITSMPQSMLQHSG
                     WNGINFNSPFADLAILLGINWLAILLYIEAFVSPFGTGVSFVAVTGRVLRAMEKNGHI
                     PKFLGKMNEKYHIPRVAIIFNAIISMIMVTLFRDWGTLAAVISTATLVAYLTGPTTVI
                     ALRKMGPTMTRPFRAKILKVMAPLSFVLASLAIYWAMWPTTAEVILIIILGLPIYFFY
                     EYRMNWRNTKKQIGGSLWIIVYLIVLSILSFIGSKEFKGLNMIHYPFDFIVIIIVALI
                     FYYIGTTSSFESVYFRRATRINTKMRESLNNESKSSH"
     gene            2369..3721
                     /locus_tag="O0293_002342"
     CDS             2369..3721
                     /locus_tag="O0293_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HCY0281213.1"
                     /translation="MKINTTGGQIHGITQDGLDIFLGIPYAEPPVHDNRFKHSTLKTQ
                     WSEPIDATEIQPIPPQPDNKLEDFFSSQSTTFTEHEDCLYLNIWKQHNDQTKKPVIIY
                     FYGGSFENGHGTAELYQPAHLVQNNDIIVITCNYRLGALGYLDWSYFNKDFHSNNGLS
                     DQINVIKWVHQFIESFGGDANNITLMGQSAGSMSILTLLKIPDIEPYFHKVVLLSGAL
                     RLDTLESARNKAQHFQKMMLDYLDTDDVTSLSTNDILMLMAKLKQSRGPSKGLDLIYA
                     PIKTDYIQNNYPTTKPIFACYTKDEGDIYITSEQKKLSPQRFIDIMELNDIPLKYEDV
                     QTAKQQSLAITHCYFKQPMKQFLQQLNIQDSNAQLWLAEFAWHDTSSAHYRSAYHILD
                     MVFWFGNLQILAAHQYPTTAHLKFLSRQMQNDLANFAKSGKMPWPMYHNERRYYRTYQ
                     "
     gene            complement(3783..4970)
                     /locus_tag="O0293_002343"
     CDS             complement(3783..4970)
                     /locus_tag="O0293_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0281214.1"
                     /translation="MSIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQL
                     VTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRIIS
                     SAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYT
                     FLFLIIVSIIVGFLMMIYLPKDQEIQRGPVNHETPSHENHVTSKILRPAEVAKYLIIT
                     FLVLIANSVTFVFINPLILSNGHDMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRW
                     LMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQV
                     MSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVVFTLKNNHYAKTF
                     KSS"
BASE COUNT         1725 a          933 c          837 g         1629 t
ORIGIN      
        1 taaaaaacta attttacaaa tgcttttgcg ttcttacaaa aaatgcattt gactattatt
       61 ataataagcg tataattgtc gcatattatt ttttgtattt ttggcaataa cgaaggagta
      121 tttatgaata aagacaagca attgcacaac gacaaaatca atctatccca attagtctta
      181 ttagggttag gctctttaat aggatctggt tggctatttg gtgcgtggga agcatcatca
      241 atagctggac cagcagcaat catatcatgg gttcttggat tcctagtcat tggaaccatt
      301 gcctataact acattgaaat cggcacaatg tttcctcaat caggtggcat gagtaactat
      361 gcccagtata cacatggctc attattaggc tttattgctg cttgggcgaa ttgggtgtct
      421 ttggtgacaa taatacctat cgaagctgtg tcagctgttc aatatatgag ttcttggccg
      481 tggcattggg cgaaaccaat gagatattta atggaaaatg gctctattag cacatacgga
      541 ttgctagctg tatatctcat cattgttatt ttttcattat taaactattg gtccgtaaaa
      601 cttttaacat catttacgag tttaatttct gtatttaaat taggcgtacc catgttaacc
      661 atcatcatgt tgatgctatc aggattcgac acttcaaatt acggccattc ggcaagcaca
      721 tttatgcctt acggaagtgc accgattttt gctgcaacaa cagcatcagg gattattttt
      781 tcattcaatt cattccagac aattattaat atgggttcag aaattaaaaa tcctgaaaaa
      841 aatatcgcaa gaggcatcgc tatctcactg tcaatcagtg cagtgttgta catcatttta
      901 caaagtacgt ttatcacttc tatgcctcaa tcaatgttac aacatagtgg atggaatggc
      961 atcaacttca attcaccatt tgctgattta gctatcttat taggaattaa ttggctcgca
     1021 attttactat acattgaagc ttttgtatca ccattcggta ctggcgtgtc atttgtcgcc
     1081 gttacaggtc gagttttacg agcaatggag aaaaatggac atatccctaa atttcttggg
     1141 aagatgaatg aaaaatatca tatcccacgt gtagcaatca tctttaatgc catcattagt
     1201 atgattatgg ttacattatt tagagattgg ggtacgctag cagcagttat ttctactgca
     1261 actttagtag cctatttaac tggcccaacg acagtgattg cattaagaaa aatgggacca
     1321 acaatgactc gtccatttag agcaaaaatt ttaaaagtaa tggcaccatt atcatttgta
     1381 ttagcttcat tagctatata ttgggcaatg tggccaacaa cggctgaagt tattttaatc
     1441 attatacttg gattaccaat ctacttcttc tatgaatatc gtatgaattg gcgtaataca
     1501 aagaaacaaa ttggtggtag cttatggatt attgtatatt taatcgtgct atcaatactg
     1561 tcatttatag gaagcaaaga atttaaaggc ttaaatatga ttcactatcc atttgacttt
     1621 atcgttatta ttattgtggc acttatcttc tattacatcg gtacaacgag ttcatttgaa
     1681 agcgtctatt tccgtcgcgc aacacgaatc aatacgaaga tgcgtgagtc actaaataat
     1741 gaatcaaagt catctcatta aatattttaa aatacagtgc catatatgat ttcaacacac
     1801 acacattaac caaccattga tttcaacatc ttggttggtt ttttattttg aaaatcggtt
     1861 ataaataact aacataacaa gatgatgatc aggctgggac ataaatcaat gttctatgct
     1921 ctacgaagtt atattggcag tagttgactg aacgaaaatg cgcttgtaac aagctttttt
     1981 cgattctagt caggggcccc aacacagaga atttcgaaaa gaaattctac aggcaatgca
     2041 agttggggtg ggacgacgat aaagaaatac tttttctata gaaattagta tttcttatgc
     2101 atgagtttta ctcatgtatt catattttta agtacacatt agctgtggct aatgtataag
     2161 aaccactaca taataaatca tttgtggctc tttatcattt ctgtcccact cccgtagaag
     2221 tacatcatat aatgctgaaa atggtttgag ttaaaacaga tatcaagctc gtctgattca
     2281 gtcacaaaat tgtcttgtta tacttgtcac ctatcatcta tagaccgtgg tatgattaaa
     2341 ttggggatga taaaggaggt taataaatat gaagattaat actacaggtg gtcaaattca
     2401 tggtattaca caagatggtt tagatatctt cttaggcatt ccttatgcag aaccaccagt
     2461 tcatgacaat cgctttaaac attctacgtt aaaaacacaa tggtcagagc caattgatgc
     2521 aactgaaata caacccatcc caccgcaacc agacaacaaa ttagaagatt ttttctcctc
     2581 acaatctaca acttttactg aacatgaaga ctgtttatat ctaaatattt ggaaacaaca
     2641 taatgatcag acgaagaaac ctgtcatcat ttatttttat ggtggtagtt ttgaaaatgg
     2701 tcatggtaca gccgaactct atcaaccggc acatttagta caaaataacg acattatcgt
     2761 tattacatgc aattatcgtt taggcgcatt aggatattta gactggtcat attttaataa
     2821 agattttcat tccaataatg gcctttcaga tcaaatcaat gtcataaaat gggtgcatca
     2881 atttattgaa tccttcggtg gcgacgctaa taacattact ttaatgggtc agtctgcagg
     2941 cagtatgagc attttgactt tacttaaaat acctgacatt gagccatact tccataaagt
     3001 cgttctacta agtggcgcac tacgattaga cacccttgag agtgcacgca ataaagcaca
     3061 acatttccaa aaaatgatgc tcgattattt agatacagat gatgttacat cattatcgac
     3121 aaatgatatt cttatgctga tggcgaagct aaaacaatct cgaggacctt ctaaagggct
     3181 tgatttaata tatgcgccta ttaaaacaga ttatatacaa aataattatc caacaacgaa
     3241 accaattttt gcatgttata caaaagatga aggcgatatt tatattacta gtgaacagaa
     3301 aaaattatcg ccgcaacgct ttatcgacat tatggaatta aatgatattc ctttaaaata
     3361 cgaagatgtt cagacggcga agcaacaatc tttagcgatt acacattgtt atttcaaaca
     3421 gccgatgaag caatttttac aacaactcaa tatacaagat tcaaacgcac aactatggct
     3481 tgctgaattt gcatggcatg atacttctag cgcacactat cgcagtgctt accatattct
     3541 agatatggta ttttggttcg gaaacttaca aatattagca gcacatcaat atccaacaac
     3601 tgctcacctt aaatttttaa gtcgacaaat gcaaaatgat ttagctaatt ttgctaaaag
     3661 cggtaaaatg ccttggccga tgtatcataa tgaacgtcgc tattatcgca catatcaata
     3721 acgttatcgc atctaaaaaa gcacacctga atttgatagc aggcgtgctt ttcatatgag
     3781 aattatgatg atttaaatgt tttagcataa tgattatttt tcaatgtgaa aacaacgatt
     3841 aatcctaatg cgccaatgat ggcactcgta tatgtgatag cttgaacaga aacatgtgtc
     3901 atgaccaagc ctccaataat gcctccaaca ccaataccag cgtttaaact agacatgttc
     3961 caactcatta cttggcttgt gtcgccttca acatgttgaa tcacaccgct ttgcactgct
     4021 ggattagtac tccattgcat gatattccaa ataaatagtc ctgctaacaa tagacctgaa
     4081 ccaggtaaga ttaaattcat aagtaacatc atgacgataa aaatagaaac agaaatcatt
     4141 aaccaacgct tacttgtaat tttatcggag aatataccac ctaatgatgt tccaataacg
     4201 ccagcgattc catttactag aagtgctaat gaaacgaatg acatatcatg accattagat
     4261 aaaataagtg gatttataaa gacgaatgtc actgagtttg caatcaatac taaaaacgta
     4321 ataattaaat attttgctac ttcagcaggt cttaatattt tcgaagtaac atgattttca
     4381 tgagatggtg tctcatgatt cacagggcct cgttgtattt cctgatcctt cggtaaatag
     4441 atcatcatca agaagccaac aataatactc acaataatta agaatagaaa tgtatagcgc
     4501 caacctacta aatcgccgat aaccgttcca attggtacac caaaaacatt agcaccacta
     4561 aaccctgtat agacaagtcc aatcatttta ccacgatttt taggtgctga taacatcgct
     4621 gtaatagcta atacttttac gataattagt gctgctgctg cagatgagat aattctacca
     4681 actactaata ttgaaaaatt tggcgctaca gcaataatgc cattaccaat gataaatata
     4741 agtaatgtcc ataataatac aggccttgat gaaaaacggt tcgttaattt aaccagaata
     4801 ggtccacata tcgcaaatgt taaagcgtac attgtcacta attgaccaac gacagcttct
     4861 gatacatgta agtcctgact catcaagttc ataattcctg caaccatcat ttcaaccatt
     4921 cctacgataa aaatacttaa aatgaatgta aatagtcgca tgattgacaa tttaaaactc
     4981 ctttcaaaca aaatttccga tattcatttc ccttagaata acgatttatt aaaaaatgtg
     5041 tgcaacacaa ttttatatat aaccgttcta ttatataata ttttgcttta attttctgtg
     5101 cttaattgga aaattattga aatt
//
LOCUS       DAKTIO010000172         5102 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.174, whole genome shotgun sequence.
ACCESSION   DAKTIO010000172 DAKTIO010000000
VERSION     DAKTIO010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5102
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.174"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(15..90)
                     /locus_tag="O0293_002344"
     tRNA            complement(15..90)
                     /locus_tag="O0293_002344"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(55..57),aa:Ala,seq:tgc)
     gene            complement(114..187)
                     /locus_tag="O0293_002345"
     tRNA            complement(114..187)
                     /locus_tag="O0293_002345"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(151..153),aa:Pro,seq:tgg)
     gene            complement(208..284)
                     /locus_tag="O0293_002346"
     tRNA            complement(208..284)
                     /locus_tag="O0293_002346"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(248..250),aa:Arg,seq:acg)
     gene            complement(290..378)
                     /locus_tag="O0293_002347"
     tRNA            complement(290..378)
                     /locus_tag="O0293_002347"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(342..344),aa:Leu,seq:taa)
     gene            complement(386..460)
                     /locus_tag="O0293_002348"
     tRNA            complement(386..460)
                     /locus_tag="O0293_002348"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(426..428),aa:Gly,seq:gcc)
     gene            complement(494..566)
                     /locus_tag="O0293_002349"
     tRNA            complement(494..566)
                     /locus_tag="O0293_002349"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(531..533),aa:Lys,seq:ttt)
     gene            complement(573..648)
                     /locus_tag="O0293_002350"
     tRNA            complement(573..648)
                     /locus_tag="O0293_002350"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(613..615),aa:Thr,seq:tgt)
     gene            complement(665..740)
                     /locus_tag="O0293_002351"
     tRNA            complement(665..740)
                     /locus_tag="O0293_002351"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(705..707),aa:Val,seq:tac)
     gene            complement(753..867)
                     /gene="rrf"
                     /locus_tag="O0293_002352"
     rRNA            complement(753..867)
                     /gene="rrf"
                     /locus_tag="O0293_002352"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(1425..2912)
                     /gene="lysS"
                     /locus_tag="O0293_002353"
     CDS             complement(1425..2912)
                     /gene="lysS"
                     /locus_tag="O0293_002353"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCY0281215.1"
                     /translation="MSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEW
                     DQYSKEELVEKEADSHVAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDDEF
                     DLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYR
                     QRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTH
                     HNALDATLYMRIAIELHLKRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAY
                     ADYHDIMDLTESMVRHIANEVLGSAKVQYNGETIDLESAWTRLHIVDAVKEATGVDFY
                     EVKSDEEAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTFIYGHPTEISPLA
                     KKNPEDPRFTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDE
                     DYIEALEYGMPPTGGLGIGIDRLVMLLTDSPSIRDVLLFPYMRQK"
     gene            complement(2965..3057)
                     /locus_tag="O0293_002354"
     CDS             complement(2965..3057)
                     /locus_tag="O0293_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281216.1"
                     /translation="MVNDKVLETSKEMYVEQKCLIFYKTLKENV"
     gene            complement(3451..3927)
                     /gene="folK"
                     /locus_tag="O0293_002355"
     CDS             complement(3451..3927)
                     /gene="folK"
                     /locus_tag="O0293_002355"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCY0281217.1"
                     /translation="MIQAYLGLGSNIGDRESQLNDAIKILNEYDGINVSNISPIYETA
                     PVGYTEQPNFLNLCVEIQTTLTVLQLLECCLKTEECLHRIRKERWGPRTLDVDILLYG
                     EEMIDLPKLSVPHPRMNERAFVLIPLNDIAANVVEPRSKLKVKDLVFVDDSVKRYK"
     gene            complement(3924..4289)
                     /gene="folB"
                     /locus_tag="O0293_002356"
     CDS             complement(3924..4289)
                     /gene="folB"
                     /locus_tag="O0293_002356"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HCY0281218.1"
                     /translation="MQDTIFLKGMRFYGYHGALSAENEIGQIFKVDVTLKVDLAEAGR
                     TDNVIDTVHYGEVFEEVKSIMEGKAVNLLEHLAERIANRINSQYNRVMETKVRITKEN
                     PPIPGHYDGVGIEIVRENK"
     gene            complement(4267..5070)
                     /gene="folP"
                     /locus_tag="O0293_002357"
     CDS             complement(4267..5070)
                     /gene="folP"
                     /locus_tag="O0293_002357"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCY0281219.1"
                     /translation="MTKTKIMGILNVTPDSFSDGGKFNNVESAINRVKAMIDEGADII
                     DVGGVSTRPGHEMVSLEEEMNRVLPVVEAIVGFDVKISVDTFRSEVAEACLKLGVDMI
                     NDQWAGLYDHRMFQIVAKYDAEIILMHNGNGNRDEPVVEEMLTSLLAQAHQAKIAGIP
                     SNKIWLDPGIGFAKTRNEEAEVMARLDELVATEYPVLLATSRKRFTKEMMGYDTTPVE
                     RDEVTAATTAYGIMKGVRAVRVHNVELNAKLAKGIDFLKENENARHNLS"
BASE COUNT         1563 a         1115 c          713 g         1711 t
ORIGIN      
        1 gtgtaaaaaa taaatggtgg agactagcgg gatcgaaccg ctgacctcct gcgtgcaaag
       61 caggcgctct cccagctgag ctaagccccc ataaaatgga atttaagaag taatcgggaa
      121 gacaggattc gaacctgcga ccccttggtc ccaaaccaag tgctctacca agctgagcta
      181 cttcccgtta ttcaattaat ttaaaaatgg cgcgcccgat aggagtcgaa cccataacct
      241 cttgatccgt agtcaaacgc tctatccaat tgagctacgg gcgctaaaat ggtgccgagg
      301 accggaatcg aaccggtacg gtgatctctc accgcaggat tttaagtcct gtgcgtctgc
      361 cagttccgcc accccggcaa aataatggag cagaagacgg gattcgaacc cgcgacccca
      421 accttggcaa ggttgtattc taccgctgaa ctacttctgc attattttgc taaatataaa
      481 aattaaatcg taatgagcca tagaggattc gaacctctga ccctctgatt aaaagtcaga
      541 tgctctacca gctgagctaa tggctcaaaa gatggtgccg gccagaggac ttgaaccccc
      601 aacctactga ttacaagtca gttgctctac caattgagct aggccggcta tatctaagaa
      661 taaatggtgg agaatgacgg gttcgaaccg ccgaccctct gcttgtaagg cagatgctct
      721 cccagctgag ctaattctcc gatttgtcat ttgcctggca acgttctact ctggcggaac
      781 gtaagttcga ctaccatcga cgctaaggag cttaacttct gtgttcggca tgggaacagg
      841 tgtgacctcc ttgctatagt caccagacaa aataacttac ttattcatga tacacgctta
      901 attaataagg tgcaatagtt aattttacac tttgatgtaa actttgtcac atcagctttt
      961 agtgtgtttc gtctgttttt ggcgactttt atatattact tcattcaatt gataaagtca
     1021 acacttttaa tcaacttttt tattcttcaa aactgacatt tctcgtgccc tctaattcat
     1081 aacactttta gtattctatc atacacatat gtacaaaacc atacacattc tatacaattt
     1141 taaagtactt ttatagctga ttaagataaa ttatactaat atagcaattt ttgttataag
     1201 atatctcaat gtctatcgtt aacatttctc atccgaacta ctctatatac gtatcgtcat
     1261 tttcatctat cagccaacta ccttgtttaa cagtcaaaaa tagcaccact atcgttaact
     1321 attactatca aaaacattca aacacgaact aattaccata attaagaaag ccctcgcata
     1381 cacatgttgt atacacgaga gctcttacta acaatcaacg tcatttattt ttgtctcata
     1441 taagggaata ataatacgtc tctgattgat ggagagtcag ttaataacat aaccaatctg
     1501 tcaataccga taccaagacc acctgtcgga ggcataccat attctaacgc ttcaatgtaa
     1561 tcttcatcca tttcatgcgc ttcatcatta ccttgcgctt tttcaacaag ttgcgcttca
     1621 aaacgacctt tttgatcaat aggatcattt aattcagtaa atgcatttgc atgctctcta
     1681 cctacaatga acaattcgaa acgatcagta aatctaggat cttcaggatt tttcttcgct
     1741 aaaggtgaaa tttcagtcgg atgaccatag ataaacgttg gctgaataag tgtttcttca
     1801 actttttgct caaagaattc atttaaaata tgaccatatt tcattgtatc tttaatttca
     1861 ataccatgtt ctttagctaa agctttagct tcttcatcac ttttaacttc ataaaaatct
     1921 acaccagtag cttcttttac agcatcaaca atatgcaaac gagtccaagc agattctaaa
     1981 tcgatcgttt ccccattgta ttgtactttt gcagaaccta acacttcatt ggcaatatgt
     2041 ctcaccatag attctgttaa atccataatg tcatgataat ctgcatatgc ttcatataat
     2101 tcaatcattg tgaattcagg gttatgtcta gttgatacac cttcattacg gaatactcta
     2161 ccaatttcat atactttttc aagtccaccg acaattaaac gttttaaatg caactcaata
     2221 gcaatacgca tgtataacgt tgcatctaat gcattatgat gtgttacaaa tggtctagca
     2281 gctgctccac cagcaatttg gtgcatcata ggtgtttcta cttccaagaa acctttatta
     2341 tttaaataat tacgcatttc ttgaatgatt ttactacgat taataaatgt acgagtgcta
     2401 tcttcgttcg taattaaatc taaatatctt tgacgatatc tctgttcaat atcctgtaaa
     2461 ccgtggaatt tatccggtaa tggtcgcaat gatttagtta gtagcgtgaa tttcttcgct
     2521 ttaaccgata attcgccagt atttgttttg aacattacac cttcaacacc aacgatatcg
     2581 cctaaatcag catttttcca taaatcaaat tcgtcatcgc caacttgatc tttacgaacg
     2641 taaatttgaa tttgtccagc taagtcctga acgtgtgcaa atcctgcttt acctttacca
     2701 cgcttagtca ttaatcgtcc agctatagcg acatgactat ccgcttcttt ttctaccaat
     2761 tcttctttag aatactggtc ccactcttct ttcaaatcac tagataaacc tgaacggtca
     2821 aatttagaac caaacgggtc tataccaaga tcatataatt cttgtaattt ttgacgtcga
     2881 accaacattt ggtcattcat ttcttctgac ataactttct ctcctttaac ttgttgtaat
     2941 cctttagtat tccaactggt acactcaaac attttccttt aaagttttat aaaatatcag
     3001 acatttttgc tcaacataca tctctttcga tgtttctaat actttatcat taaccattac
     3061 accttaaagt tatgatgtgg catgttttct tatattcata acatcaattt tatcactttt
     3121 ttaattttac aagagcttgt aaatgtaata aagcaatttc tgttttcgtt tatgaattac
     3181 ttattcattt aaaatacgat tatcattaat acaaccctaa gtaaatgtat aaaaattatc
     3241 ttccacaaac ttcaacaaaa gcctaaataa attacagcaa tttatcaaat attgcttact
     3301 ttgattttat gaaataactt aattctaaca catactaaat catcatatac taattcgaaa
     3361 tcaaatgcat ttagagataa tcaaaatgcg gaaacatctc caataatcaa taatctattc
     3421 ccaataaata tgaatgttct caacaataca ttatttatat ctctttacac tgtcatcgac
     3481 aaaaactaaa tctttcactt tcaatttcga acgtggttct acgacatttg ctgctatatc
     3541 atttaatggg attaaaacaa atgcacgttc attcattctc ggatgtggca ccgacagttt
     3601 tggtaaatct atcatttctt ctccatacaa caaaatatcc acatctaaag ttctaggacc
     3661 ccatcgttcc tttctaatac ggtgtaaaca ttcttctgtc ttcaaacaac attccaacag
     3721 ttgtaatact gtgagtgttg tttgaatttc aacacacaaa tttaaaaagt taggttgctc
     3781 agtataccca actggtgctg tttcataaat cggagaaata ttagatacgt taataccatc
     3841 atattcattc aaaatcttta tagcatcgtt taactggctt tctctatcac caatattact
     3901 acctaatcct aagtatgctt gaatcattta ttctccctca ctatttcgat acctactcca
     3961 tcataatgac ccggaatcgg tgggttttct ttagtgattc tcactttcgt ttccattaca
     4021 cgattatatt gtgaatttat acgatttgca atacgttcag ctagatgctc aagtaaatta
     4081 acggccttac cttccataat tgatttaacc tcttcgaaca cttcaccata atgaactgta
     4141 tcaataacat tatcagtacg cccagcttca gctaagtcta ctttcaaagt tacatccact
     4201 ttgaaaattt gccctatttc attttcagct gataaagcac catgatatcc ataaaagcgc
     4261 atacctttaa gaaagattgt gtcttgcatt ttcattctcc tttaaaaaat ctataccttt
     4321 agctaattta gcattcaact cgacattatg aacgcgtact gctctaacgc ctttcataat
     4381 accatatgca gtcgtagctg cagttacttc atctctttca accggtgttg tatcataacc
     4441 catcatctct ttagtgaaac gtttccggct tgtcgctaat aaaactggat attctgttgc
     4501 aacaagttca tccagtcttg ccataacttc ggcttcttca tttctagttt tagcgaaacc
     4561 tatacctgga tctagccaaa ttttatttga aggtatacca gctattttag cttgatgtgc
     4621 ttgtgctaac aaagatgtta acatttcttc gacaaccggt tcatcacgat taccatttcc
     4681 attatgcatt aaaataattt ccgcgtcata tttagctaca atttggaaca tacgatgatc
     4741 atacagaccc gcccattgat cattaatcat atcaacgcct aattttaaac atgcttcagc
     4801 aacctcactt cgaaatgtat cgactgaaat ttttacatca aaaccgacaa tagcttcaac
     4861 aacaggtaat actctgttca tctcttcttc taatgaaacc atttcatgac cgggtctcgt
     4921 tgaaacacct ccaacatcta taatgtcagc accttcatct atcatggctt tcactctatt
     4981 tatagctgat tcaacattat taaattttcc accatctgag aatgaatcag gtgtgacgtt
     5041 taatatgccc ataatttttg ttttagtcat gttaaccacc ctttaattat gtattgttta
     5101 ac
//
LOCUS       DAKTIO010000173         5066 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.175, whole genome shotgun sequence.
ACCESSION   DAKTIO010000173 DAKTIO010000000
VERSION     DAKTIO010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5066
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.175"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(215..1921)
                     /gene="acsA"
                     /locus_tag="O0293_002358"
     CDS             complement(215..1921)
                     /gene="acsA"
                     /locus_tag="O0293_002358"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCY0281220.1"
                     /translation="MKVEVYKGAQGKHNLKDYEETYNTFDWKDVEQAFSWSETGKMNM
                     AYECIDRHVDQGLGDKIALNYKDEHRKESYTYKDMQRLSNKAANVLSEHAEVDKGDRV
                     FIFMSRTPELYFALLGVLKIGAIVGPLFEAFMEKAVADRLENSEAKVLITNKALLPRV
                     PVDKLPNLKKIVVVDEDVEDNYIDFISLMETASDEFDIEWLKSDDGLILHYTSGSTGQ
                     PKGVLHVQQAMLVHYISGKYVLDLQEDDVYWCTADPGWVTGTSYGIFAPWLNGATNCI
                     AGGRFSPEQWYSMIEDFKVTIWYTAPTALRMLMSAGDDIVEKYDLSSLRSILSVGEPL
                     NPEVIKWAKKVYGLTVLDTWWMTETGGHMIVNYPTMDVKLGSMGKPLPGIQAAIIDDA
                     GNELPPNRMGNLAIKKGWPSMMYRIWKNPEKYKSYFIGDWYVSGDSAYKDEDGYFWFQ
                     GRVDDVIMTAGERVGPFEVESKLVEHEAVAEAGIIGKPDPVRGEIIKAFVALRKGYEP
                     TDELKEEIRIFVKEGLSAHAAPREIEFKDKLPKTRSGKIMRRVLKAWELNLDAGDLST
                     ME"
     gene            2090..2722
                     /locus_tag="O0293_002359"
     CDS             2090..2722
                     /locus_tag="O0293_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCY0281221.1"
                     /translation="MNHLKTYQSEDYYIHDKQFVIEGPLTHEDLKALTFDAHLTAFRD
                     AEDQYKALLEITTLPEGRIYVARQDQLIVGYVTFHYPDEIERWSTGKLPYLIELGAIE
                     VSINFRQLQLAEKLIQLSLSTPEFENYIVITTEYYWHWDLKNSKLDVFDYKKLMQRLM
                     ATGGLEIFATDDPEITSHPANCLMARIGKNITLEQQQAFDDIRYMNRFFF"
     gene            2747..3916
                     /locus_tag="O0293_002360"
     CDS             2747..3916
                     /locus_tag="O0293_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HCY0281222.1"
                     /translation="MQQHSSKTAYVYSDKLLQYRFHDQHPFNQMRLKLTTELLLNANL
                     LSPEQIVQPRIATDDELMLIHKYDYVEAIKHASHGIISEDEAKKYGLNDEENGQFKHM
                     HRHSATIVGGALTLADLIMSGKVLNGCHLGGGLHHAQPGRASGFCIYNDIAITAQYLA
                     KEYNQRVLIIDTDAHHGDGTQWSFYADNHVTTYSIHETGKFLFPGSGHYTERGEDIGY
                     GHTVNVPLEPYTEDASFLECFKLTVEPVVKSFKPDIILSVNGVDIHYRDPLTHLNCTL
                     HSLYEIPYFVKYLADSYTNGKVIMFGGGGYNIWRVVPRAWSHVFLSLIDQPIQSGYLP
                     LEWINKWKHYSSELLPKRWEDRLNDYTYVPRTKEISEKNKKLALHIASWYESTRQ"
     gene            complement(4014..5003)
                     /gene="ccpA"
                     /locus_tag="O0293_002361"
     CDS             complement(4014..5003)
                     /gene="ccpA"
                     /locus_tag="O0293_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HCY0281223.1"
                     /translation="MTVTIYDVAREARVSMATVSRVVNGNQNVKAETKNKVNEVIKRL
                     NYRPNAVARGLASKKTTTVGVIIPDISNIYYSQLARGLEDIATMYKYHSIISNSDNDP
                     EKEKEIFNNLLSKQVDGIIFLGGTITEEMKELINQSSVPVVVSGTNGKDAHIASVNID
                     FTEAAKEITGELIEKGAKSFALVGGEHSKKAQEDVLEGLTEVLNKNGLQLGDTLNCSG
                     AESYKEGVKAFAKMKGNLPDAILCISDEEAIGIMHSAMDAGIKVPEELQIISFNNTRL
                     VEMVRPQLSSVIQPLYDIGAVGMRLLTKYMNDEKIEEPNVVLPHRIEYRGTTK"
BASE COUNT         1604 a          980 c          744 g         1738 t
ORIGIN      
        1 atcttccaaa gatcatatta aaaataaaaa gcaatataaa gatatgccat tattgttatg
       61 tcatcatttt caaatgcttt ctacgaaatg atatgacaca agttaacagt tctttatatt
      121 gcttatcgta aagtaaacga ttatttaaac attgacatga aatatgttgt caccctttat
      181 tcttcgaaaa aaatcttcaa taacattcat gtcattattc cattgtactt aaatccccag
      241 catctaaatt taattcccaa gcttttaata cacgtctcat aattttacct gaccgtgttt
      301 taggtaattt atctttaaat tcgatttcac gtggtgctgc atgtgccgac aaaccttctt
      361 taacaaatat acgaatttct tcttttaatt cgtctgttgg ttcatatcct tttctcagtg
      421 caacaaacgc cttaattatt tcaccgcgaa ccggatcagg tttaccaata attcctgctt
      481 cggcaactgc ttcgtgttca accaatttag actcaacctc aaatggtcca actcgttcac
      541 cagctgtcat aattacatca tcaacacgtc cttggaacca gaagtaacca tcttcatctt
      601 tatatgccga atcaccagat acataccagt ctccaataaa atatgattta tatttttctg
      661 gattcttcca gatacgatac atcattgatg gccagccttt ttttatagca aggttgccca
      721 ttcgatttgg tggtaattca ttccctgcat catcgataat tgcagcttga ataccaggta
      781 atggtttgcc cattgagcca agcttgacgt ccatcgttgg atagttaaca atcatatgtc
      841 cacctgtttc tgtcatccac caagtatcta acaccgttaa accgtatact tttttcgccc
      901 attttataac ttcaggattt aaaggctcac ctactgatag aatcgaacgt aacgatgaca
      961 agtcatattt ctcaacaata tcgtcaccag cactcattaa cattcttaaa gctgttggtg
     1021 ccgtatacca aatcgtcact ttaaaatctt caatcatact ataccactgt tctggcgaaa
     1081 agcgaccacc agctatacaa tttgtagcgc catttaacca tggtgcaaaa ataccataag
     1141 atgttcctgt aacccaacct ggatctgctg tacaccaata aacatcatct tcttgtaaat
     1201 ctaatacata ttttccagaa atatagtgca ctaacattgc ttgttgaaca tgcaatacac
     1261 ctttaggttg cccagtagaa cctgatgtat aatgtaaaat caaaccatca tccgacttta
     1321 accattcaat gtcaaattca tcgctagcag tttccatcaa actaatgaag tctatgtaat
     1381 tgtcttctac atcctcatct acgacaacaa tttttttcaa gtttggtaat ttatctacag
     1441 gtactcgagg taacaatgcc ttattagtaa ttaacacttt agcttcactg ttctctaatc
     1501 tatccgcaac tgccttttcc ataaatgctt caaataacgg cccaacaatt gcaccaattt
     1561 ttaaaacacc taacaacgca aaatatagtt caggtgtacg cgacataaat ataaatactc
     1621 tgtcaccttt gtcaacttct gcatgttcag acaaaacatt cgctgcttta ttagataacc
     1681 gttgcatatc tttataagta tacgattctt ttctgtgctc atctttgtaa tttaacgcta
     1741 ttttatcccc taatccttga tctacatggc gatctatgca ttcatatgcc atgttcattt
     1801 ttccagtttc actccaagaa aatgcttgtt ctacgtcttt ccaatcaaaa gtattatatg
     1861 tttcttcata atctttaagg ttatgtttac cttgcgctcc tttataaact tcgactttca
     1921 tttgaaactc cccctttgtt atgtgaaaac gctttctttc tcaatattat acataacatt
     1981 tctaattatt caaaattttt acttttatcc tttttctagt attaattatt gtaaattttc
     2041 tatataatga atttaataca atcacgacgt cgattgagga gttgaaaata tgaatcattt
     2101 aaagacgtat caatccgaag attattacat tcatgacaag caatttgtta ttgaaggtcc
     2161 tttaacacac gaagatttga aagcgcttac tttcgatgcg catttaaccg catttagaga
     2221 tgctgaagat cagtataaag ctttgttaga aattacaaca ttaccagaag gtagaattta
     2281 tgttgctcgc caagatcaac tcattgtggg ttatgtcact ttccactatc ctgatgaaat
     2341 tgagcgctgg tctacaggta agcttccata tttaatcgaa ttgggggcaa ttgaagtcag
     2401 catcaatttt aggcaattac aacttgcaga aaagctgata caacttagcc tttctacacc
     2461 agaattcgag aattatatcg ttataactac agaatattac tggcattggg atttaaaaaa
     2521 ttcaaagtta gatgtatttg actataaaaa attaatgcag cggttaatgg caactggtgg
     2581 acttgaaata ttcgctacag atgatccaga aataacaagt catccagcta attgtttaat
     2641 ggcaagaatt ggcaaaaaca ttacattaga acagcaacaa gcgtttgatg atattcgtta
     2701 tatgaatcgg tttttctttt aatttttgga atatcggagg gattatatgc aacaacattc
     2761 atcaaaaact gcatatgttt attcagataa gttattacaa tatcgatttc atgaccaaca
     2821 tcccttcaat caaatgcgtt taaaattaac aacagagcta cttttgaatg caaatttatt
     2881 gtctccagaa caaatagtac aacctagaat tgcaacagat gacgaattaa tgttaattca
     2941 taaatatgat tacgtcgaag ctattaagca tgcttcacat ggcattatca gtgaagatga
     3001 ggctaagaaa tatggattaa atgatgaaga gaatggtcaa tttaagcata tgcaccgcca
     3061 tagtgccaca attgttggag gcgctttaac tttagcagat cttattatgt caggcaaagt
     3121 attaaatggt tgtcacttag gtggtggttt gcatcacgct caacctggtc gagcaagtgg
     3181 tttttgtata tacaatgata ttgcaattac cgcacaatac ttagctaaag aatacaatca
     3241 acgcgtttta atcatagata ccgatgcaca tcatggagat ggtacacaat ggagtttcta
     3301 tgccgataac catgttacta cttattctat ccatgaaacc ggaaaatttc ttttcccagg
     3361 ctctggtcac tatactgagc gcggtgaaga tatcggctat ggacacactg taaatgtccc
     3421 acttgaaccg tatacagaag atgcatcatt tttggagtgt tttaaattaa cagttgagcc
     3481 tgtcgtaaag agttttaaac ctgatattat tctaagcgta aatggtgtcg atatacatta
     3541 tcgtgatcca ctaactcatc taaattgtac gttacattca ttatatgaaa ttccatattt
     3601 tgtaaaatat ttagctgatt cttatacgaa tggaaaggta attatgtttg gtggcggagg
     3661 ctacaatatt tggagagtcg taccacgtgc atggagtcat gtattcttaa gtttaattga
     3721 tcaaccaatt caaagtggtt atttaccgtt agaatggatt aataaatgga aacattattc
     3781 atctgaatta ttacctaaaa gatgggaaga tcgtttaaat gattatacct atgtcccccg
     3841 cacaaaagaa attagtgaaa aaaataaaaa attagcttta catatagcga gttggtacga
     3901 atctactcgt caataagtaa cctaatctgc aacgatttta aaagataaat aaaaggcatt
     3961 cttccaacac ccatataatt tgggtcgtta gatgaatgcc taattttgtg aatttatttt
     4021 gtagttcctc ggtattcaat tctgtgaggt aaaactacat ttggttcttc tatcttttca
     4081 tcgttcatat attttgttaa taagcgcatc cctactgcac cgatatcata taatggttga
     4141 ataacactag aaagttgtgg tctaaccatc tcaactaatc gtgtattatt gaaactaata
     4201 atttgtaatt cctctggaac tttaatacca gcatccattg cactatgcat aataccaatt
     4261 gcttcttcgt cgctgataca taaaatggca tctggcaaat tgcctttcat tttggcaaaa
     4321 gcttttacgc cttctttata actttcagca ccagaacaat tcaatgtatc acctaattga
     4381 aggccatttt tatttaacac ttcagttaaa ccttctaaaa catcttcttg agctttttta
     4441 gaatgttctc cacctactaa agcaaatgat ttagcgcctt tttcaattaa ttctcccgta
     4501 atttctttcg cagcttcagt aaaatcaata ttaactgatg ctatatgtgc atccttacca
     4561 tttgttcctg atactactac aggtacagat gattgattta tcaattcttt catttcttca
     4621 gtaattgtac caccaaggaa aataatacca tcaacctgtt tacttaataa gttattaaaa
     4681 atttcttttt ccttttcagg atcgttatct gaatttgaaa taattgagtg atatttatac
     4741 attgttgcaa tatcttcaag tccacgagca agttgtgaat aatagatatt agatatatct
     4801 ggaatgatca cacctactgt tgttgtcttt ttactagcta aacctctagc aacagcattt
     4861 ggacgataat tcaaacgctt aatgacttcg ttaactttat ttttagtttc tgctttaaca
     4921 ttttggttcc cattaacaac acgcgacact gtggccatag agacacgcgc ttctcttgct
     4981 acatcatata tagtaactgt cataatttcc tccttgtaaa cgttttattc attataataa
     5041 catgttttca tttcattttc atagtt
//
LOCUS       DAKTIO010000174         5040 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.176, whole genome shotgun sequence.
ACCESSION   DAKTIO010000174 DAKTIO010000000
VERSION     DAKTIO010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.176"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            29..751
                     /gene="splB"
                     /locus_tag="O0293_002362"
     CDS             29..751
                     /gene="splB"
                     /locus_tag="O0293_002362"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplB"
                     /protein_id="HCY0281224.1"
                     /translation="MNKNVVIKSLAALTILTSVTGIGTTLVEEVQQTAKAENNVTKVK
                     DTNIFPYTGVVAFKSATGFVVGKNTILTNKHVSKNYKVGDRITAHPNSDKGNGGIYSI
                     KKIINYPGKEDVSVIQVEERAIERGPKGFNFNDNVTPFKYAAGAKAGERIKVIGYPHP
                     YKNKYVLYESTGPVMSVEGSSIVYSAHTESGNSGSPVLNSNNELVGIHFASDVKNDDN
                     RNAYGVYFTPEIKKFIAENIDK"
     gene            809..1528
                     /gene="splC"
                     /locus_tag="O0293_002363"
     CDS             809..1528
                     /gene="splC"
                     /locus_tag="O0293_002363"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplC"
                     /protein_id="HCY0281225.1"
                     /translation="MNKNIVIKSMAALAILTSVTGINAAVVEETQQIANAEKNVTQVK
                     DTNIFPYNGVVSFKDATGFVIGKNTIITNKHVSKDYKVGDRITAHPNGDKGNGGIYKI
                     KSISDYPGDEDISVMNIEEQAVERGPKGFNFNENVQAFNFAKDAKVDDKIKVIGYPLP
                     AQNSFKQFESTGTIKRIKDNILNFDAYIEPGNSGSPVLNSNNEVIGVVYGGIGKIGSE
                     YNGAVYFTPQIKDFIQKHIEQ"
     gene            1649..2368
                     /gene="splD"
                     /locus_tag="O0293_002364"
     CDS             1649..2368
                     /gene="splD"
                     /locus_tag="O0293_002364"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplD"
                     /protein_id="HCY0281226.1"
                     /translation="MNKNIIIKSIAALTILTSITGVGTTVVDGIQQTAKAENSVKLIT
                     NTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYK
                     VTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPN
                     GNKLQMYESTGKVLSVNGNIVTSDAVVQPGSSGSPILNSKREAIGVMYASDKPTGEST
                     RSFAVYFSPEIKKFIADNLDK"
     gene            complement(2317..2424)
                     /locus_tag="O0293_002365"
     CDS             complement(2317..2424)
                     /locus_tag="O0293_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281227.1"
                     /translation="MLILLLISKNHLSMYGWMIYLSKLSAINFLISGEK"
     gene            2526..3242
                     /gene="splE"
                     /locus_tag="O0293_002366"
     CDS             2526..3242
                     /gene="splE"
                     /locus_tag="O0293_002366"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplE"
                     /protein_id="HCY0281228.1"
                     /translation="MNKNIIIKSIAALTILTSVTGVGTTVVEGIQQTAKAEHNVKLIK
                     NTNVAPYNGVVSIGSGTGFIVGKNTIVTNKHVVAGMEIGAHIIAHPNGEYNNGGFYKV
                     KKIVRYSGQEDIAILHVEDKAVHPKNRNFKDYTGILKIASEAKENERISIVGYPEPYI
                     NKFQMYESTGKVLSVKGNMIITDAFVEPGNSGSAVFNSKYEVVGVHFGGNGPGNKSTK
                     GYGVYFSPEIKKFIADNTDK"
     gene            3393..4112
                     /gene="splF"
                     /locus_tag="O0293_002367"
     CDS             3393..4112
                     /gene="splF"
                     /locus_tag="O0293_002367"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplF"
                     /protein_id="HCY0281229.1"
                     /translation="MNKNIIIKSIAALTILTSITGVGTTMVEGIQQTAKAENTVKQIT
                     NTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYK
                     VTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPN
                     GNKLQMYESTGKVLSVNGNIVSSDAIIQPGSSGSPILNSKHEAIGVIYAGNKPSGEST
                     RGFAVYFSPEIKKFIADNLDK"
     gene            4177..4311
                     /locus_tag="O0293_002368"
     CDS             4177..4311
                     /locus_tag="O0293_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281230.1"
                     /translation="MHYLICIKANQRLIFTNSIVDWFYLCMNEQLFDIIKNINDFESI
                     "
     gene            4475..>5040
                     /locus_tag="O0293_002369"
     CDS             4475..>5040
                     /locus_tag="O0293_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HCY0281231.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFL"
BASE COUNT         1959 a          760 c          889 g         1432 t
ORIGIN      
        1 caaaataatt aaaagagagg aacttaaaat gaacaaaaac gtagtcatca agagtttagc
       61 agcattaaca attttaacat ctgtaacagg tattggaaca acattggttg aggaagtaca
      121 acaaactgcc aaagcagaaa ataatgtcac aaaagttaaa gatactaata tttttccata
      181 tactggtgta gttgctttta aaagtgcaac tggatttgta gttggaaaga atactatttt
      241 aacaaataaa catgtgtcga aaaattacaa agtgggcgat cgtattactg cacatccaaa
      301 tagtgataaa ggtaatggtg gtatttattc gattaaaaag attattaatt atccaggtaa
      361 agaagatgta tcagtcattc aagttgaaga gcgtgcaata gaacgtggac caaaaggctt
      421 taattttaat gataatgtaa cgccattcaa atatgcggca ggggctaaag ctggtgagcg
      481 aattaaagtg attggttatc cacacccata caaaaataaa tatgttttat atgagtcaac
      541 tggccctgtg atgtcagtag aaggtagcag tattgtatat tcagcgcata ctgaaagcgg
      601 aaactctgga tcacctgtat taaacagcaa caacgaatta gttggtattc attttgcttc
      661 tgatgtaaaa aatgatgata acagaaatgc atatggcgtc tactttacac cagaaattaa
      721 aaaattcatt gcagaaaaca tagataaata aacaaattga ctttaaacga gcgttgcaac
      781 atatctcgaa ttgtaaagga gcttgaaaat gaataaaaat atagtcatta aaagcatggc
      841 agcattagcc attctaacct cagtaactgg aataaatgct gcagtcgttg aagagacaca
      901 acaaatagca aatgcagaga agaatgttac gcaagttaaa gatacaaata tttttccata
      961 taatggcgtc gtttcattta aagatgcgac aggttttgta attggaaaaa atacaattat
     1021 caccaataaa catgtatcaa aagattataa agttggcgat agaattactg cccatccaaa
     1081 cggtgacaaa ggaaatggtg gtatatataa aattaaaagc atttctgatt atccgggtga
     1141 tgaagacatc tctgtcatga atattgaaga acaagcagtc gaacgtggac caaaaggctt
     1201 taattttaat gaaaatgtcc aagcattcaa ttttgcgaaa gatgctaaag ttgatgacaa
     1261 aattaaagtt attggttacc cattacctgc tcaaaatagt tttaaacagt ttgaatctac
     1321 aggaactata aaaagaatca aagacaatat tttaaatttt gatgcataca ttgaacccgg
     1381 gaattcagga tcaccagttc taaattctaa caatgaggtc ataggtgtgg tgtatggcgg
     1441 tattggaaaa attggttctg aatataatgg tgccgtatac tttacgcctc aaatcaaaga
     1501 ttttattcaa aagcacattg aacaataaac aaatttaaat atacaccatg agcatgtgtt
     1561 caataatttt aatgaaaaac atcggtcgaa tataacataa aaaaacgtct atatcaaaag
     1621 catcatgaat aaacagagga gcacaaaaat gaataaaaat ataatcatca aaagtattgc
     1681 ggcattgacg attttaacat caataactgg tgtcggcaca acagtggttg atggtattca
     1741 acaaacagcc aaagcagaaa atagtgtgaa attaattacc aacacgaatg ttgcaccata
     1801 cagtggtgtt acatggatgg gcgctggaac aggatttgta gttgggaatc atacaatcat
     1861 taccaataaa catgttactt atcacatgaa agtcggtgat gaaatcaaag cacatcctaa
     1921 tggtttttat aataacggtg gtggacttta taaagttact aagattgtag attatcctgg
     1981 taaagaagat attgcggtcg tacaagttga agaaaaatca acgcaaccaa aaggtagaaa
     2041 attcaaagat ttcactagca aatttaatat agcatcagaa gctaaagaaa atgaacctat
     2101 atcagtcatt ggttatccaa atcctaatgg aaataaacta caaatgtatg aatcaactgg
     2161 taaagtacta tcagtgaatg gaaatatagt gacatctgat gcggttgtcc aacctggcag
     2221 ctctggttca cctatattaa atagtaagcg agaagcaatt ggtgttatgt atgctagtga
     2281 taaaccaaca ggtgaaagta caaggtcatt tgctgtttat ttctctcctg aaattaagaa
     2341 atttattgca gataatttag ataaataaat catccatcca tacattgata aatgattttt
     2401 agaaattaac aacaaaatca acaattttaa acatctctgt gattctattt attcgaaatg
     2461 atttaaaaaa taaaacttca aaaacctaac cttatattta tacgaatact tagaggagca
     2521 caaaaatgaa taaaaatata atcatcaaaa gtattgcagc attgacgatt ttaacatcag
     2581 tgactggcgt cggcacaaca gtggttgagg gtattcaaca aacggctaaa gctgaacata
     2641 atgtgaaact aatcaaaaat actaatgtag caccatacaa tggtgtcgtt tcgataggat
     2701 ctggaacagg tttcattgtc ggtaaaaata caattgttac caacaagcat gtcgttgcag
     2761 gtatggaaat tggtgcacat attatagcgc atcccaatgg tgaatataat aatggcggat
     2821 tttataaagt taaaaaaatt gtccgttatt caggtcaaga agatattgcc attctacatg
     2881 tggaagataa agctgttcat ccaaaaaaca ggaattttaa agattacaca ggcattttaa
     2941 aaatagcatc agaagctaaa gaaaatgaac gcatttcaat tgttggctat ccagaaccat
     3001 atataaataa atttcaaatg tatgagtcaa caggaaaagt gctgtcagtt aaaggcaaca
     3061 tgattattac tgatgctttc gtagaaccag gcaactcagg ttcagctgta tttaacagta
     3121 aatacgaagt tgtaggtgtt cactttggtg gaaacggccc tggaaataaa agtacaaaag
     3181 gatatggtgt ttatttctct cctgaaatta agaaattcat tgcagataac acagataaat
     3241 aaatccttac atagataaat gattttaaaa attaacaaca aactcaacaa ttcaaatcat
     3301 ctctgtgatt ccatttattc gaaatgatta aaaaaaataa aacttcaaaa agctaacatt
     3361 ataattatac aaatacttag aggagcagaa aaatgaataa aaatataatc atcaaaagta
     3421 ttgcagcatt gacgatttta acatcaataa ctggtgtcgg cacaacaatg gttgaaggta
     3481 ttcaacaaac agccaaagcc gaaaatactg ttaaacaaat tacaaataca aatgttgcac
     3541 catacagtgg tgttacatgg atgggcgctg gaacaggatt tgtagttgga aatcatacaa
     3601 tcattaccaa taaacatgtt acctatcaca tgaaagtcgg tgatgaaatc aaagcacatc
     3661 ctaatggttt ttataataac ggtggtggac tttataaagt tactaagatt gtagattatc
     3721 ctggtaaaga agatattgcg gttgtacaag ttgaagaaaa atcaacacaa ccaaaaggta
     3781 gaaaattcaa agatttcact agtaaattta atatagcatc agaagctaaa gaaaatgaac
     3841 ctatatcagt cattggttat ccaaatccta atggaaataa actacaaatg tatgaatcaa
     3901 ctggtaaagt attatcagtg aatgggaata tagtgtcatc ggatgcaatt attcagcctg
     3961 gtagctctgg ttcacctata ttaaatagta aacacgaagc tattggtgta atctatgccg
     4021 gtaataagcc atcaggtgaa agcacaagag gatttgctgt ttatttctct cctgaaatta
     4081 agaaattcat tgcagataat ttagataaat aattaaaact tagacattca cccaatcctg
     4141 acaaaatata ctataactaa catttattaa tatatattgc attatttaat atgcatcaaa
     4201 gccaatcaac gattgatttt caccaactca attgttgatt ggttttattt atgtatgaat
     4261 gaacaacttt ttgacatcat taagaatata aatgattttg aaagcatttg aaagctacaa
     4321 catttctata aaatttttca ataacaattg cgccactaaa actcaaaatt tccaccacca
     4381 acatccaaat tatcaacatc gcaacataac caaatgttat aataaatcta ttacacaaag
     4441 agataaatta cttatgcaaa ggcggaggaa tcacatgtct attactgaaa aacaacgtca
     4501 gcaacaagct gaattacata aaaaattatg gtcgattgcg aatgatttaa gagggaacat
     4561 ggatgcgagt gaattccgta attacatttt aggcttgatt ttctatcgct tcttatctga
     4621 aaaagccgaa caagaatatg cagatgcctt gtcaggtgaa gacatcacgt atcaagaagc
     4681 atgggcagat gaagaatatc gtgaagactt aaaagcagaa ttaattgatc aagtcggtta
     4741 cttcattgag ccacaagatt tattcagtgc gatgattcgt gaaattgaaa cgcaagattt
     4801 cgatatagaa catctagcga cggcgattcg caaagttgaa acatctacat taggtgaaga
     4861 aagtgaaaat gactttatcg ggctgttcag cgatatggat ttgagttcaa cgcgactagg
     4921 taacaatgtc aaagaacgta ctgctttaat ctctaaagtc atggttaatc ttgacgactt
     4981 accattcgtt cacagtgaca tggaaattga tatgttaggt gatgcatatg aattcctaat
//
LOCUS       DAKTIO010000175         5031 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.177, whole genome shotgun sequence.
ACCESSION   DAKTIO010000175 DAKTIO010000000
VERSION     DAKTIO010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.177"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            90..713
                     /gene="gmk"
                     /locus_tag="O0293_002370"
     CDS             90..713
                     /gene="gmk"
                     /locus_tag="O0293_002370"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCY0281232.1"
                     /translation="MDNEKGLLIVLSGPSGVGKGTVRKRIFEDPSTSYKYSISMTTRQ
                     MREGEVDGVDYFFKTRDAFEALIKDDQFIEYAEYVGNYYGTPVQYVKDTMDEGHDVFL
                     EIEVEGAKQVRKKFPDALFIFLAPPSLEHLRERLVGRGTESDEKIQSRINEARKEVEM
                     MNLYDYVVVNDEVELAKNRIQCIVEAEHLKRERVEAKYRKMILEAKK"
     gene            713..931
                     /locus_tag="O0293_002371"
     CDS             713..931
                     /locus_tag="O0293_002371"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCY0281233.1"
                     /translation="MLNPPLNQLTSQIKSKYLIATTAAKRAREIDEQPETELLSEYHS
                     FKPVGRALEEIADGKIRPVISSDYYGKE"
     gene            1147..2346
                     /gene="coaBC"
                     /locus_tag="O0293_002372"
     CDS             1147..2346
                     /gene="coaBC"
                     /locus_tag="O0293_002372"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCY0281234.1"
                     /translation="MKKILLAVTGGIAAYKAIDLTSKLTQSGYEVRVMLTNHAQKFVT
                     PLAFQAISRNAVYTDTFIEENPSEIQHIALGDWADAIIVAPATANTIAKLSVGIADDL
                     VTSTLLATETPKFIAPAMNVHMYENKRTQQNINILKEDGYHFIEPGSGFLACGYVAKG
                     RMEEPLQIVSVIDAHFQNSNRLANSSFQDKRALVTAGPTIEVIDPVRFVSNRSSGKMG
                     YAIAEALRNRGAIVTLVAGPTTLEDPKDIEVIHVQSAEEMFEQVTSRFDEQDIVVKAA
                     AVSDYTPVDVLEHKMKKQDGDLSVSFKRTKDILKYLGEHKTSQYLIGFAAETEDIENY
                     AQQKLRKKNADVIISNNVGDMSIGFSSDDNELTMHFKNNEKVNIKKGKKVVLAAQILD
                     ELETRWQ"
     gene            2346..4754
                     /gene="priA"
                     /locus_tag="O0293_002373"
     CDS             2346..4754
                     /gene="priA"
                     /locus_tag="O0293_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCY0281235.1"
                     /translation="MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRT
                     IQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTSELIALSEWMGSTHVIKRISMLE
                     VMLPSAIKAKYKKAFKMKDDIELPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDD
                     IVEEKTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFL
                     TDIEDMGFSKSSLDGLIKKGYVEKYDAVVERDPFKDRVFEQESKQQLTEDQYKAYEAI
                     KAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLVPEIALTPQMVLRFK
                     RRFGDDVAVLHSGLSNGERYDEWQKIRDGRARVSVGARSSVFAPFKNLGLIIIDEEHE
                     STYKQEDYPRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKGVYHLLSLPNRVN
                     QQALPEIDIVDMREELSEGNRSMFSKDLREAIQLRLDRQEQVVLFLNRRGYASFMLCR
                     DCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGTGTQKVE
                     ELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITL
                     VGVLNADTMLNLPDFRASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQK
                     NDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKVMVASQHVHKILLQHLTEKAL
                     VLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDP
                     QMMM"
BASE COUNT         1843 a          699 c          999 g         1490 t
ORIGIN      
        1 catgcaattt caaatatgct atacaatata aagaacaatg tgatatcata tttaaataat
       61 agaagattag cttagagagg tcgtaaggca tggataatga aaaaggattg ttaatcgttt
      121 tatcaggacc atctggagta ggtaaaggta ctgttagaaa acgaatattt gaagatccaa
      181 gtacatcata taagtattct atttcaatga caacacgtca aatgcgtgaa ggtgaagttg
      241 atggcgtaga ttactttttt aaaactaggg atgcgtttga agctttaatc aaagatgacc
      301 aatttataga atatgctgaa tatgtaggca actattatgg tacaccagtt caatatgtta
      361 aagatacaat ggacgaaggt catgatgtat ttttagaaat tgaagtagaa ggtgcaaagc
      421 aagttagaaa gaaatttcca gatgcgctat ttattttctt agcacctcca agtttagaac
      481 acttgagaga gcgattagta ggtagaggaa cagaatctga tgagaaaata caaagtcgta
      541 ttaacgaagc gcgtaaagaa gttgaaatga tgaatttata cgattacgtt gtagttaatg
      601 atgaagtaga acttgcgaag aatagaattc aatgtattgt agaagctgag cacttaaaaa
      661 gagagcgcgt agaagctaag tatagaaaaa tgattttgga ggctaaaaaa taatgttaaa
      721 tccaccttta aaccaattaa cgtcacaaat taaatcaaag tatttaattg caacaactgc
      781 agcgaaaaga gcgcgtgaaa ttgatgaaca acctgaaact gaattattaa gtgaatatca
      841 ttcatttaaa ccagttggta gagcgttaga agaaattgct gacggtaaaa ttcgccctgt
      901 tatttcaagt gattattatg gtaaagaata gtttcgacca ttaaaaatat gtgaagctag
      961 acgtacatca aatacgtcaa ggcatatcca aatcaaacgc acctatgtag gtgtgttttt
     1021 ttagtttatc caatgattga tgttataata atactaaatt tgtatctata aaaaagtaat
     1081 gagcatttgt gcgcatatga tgatgtaaag cgtaaatgtc atagtgatat ttaagggaga
     1141 ttgaatatga agaaaatatt attagccgtt acaggtggca ttgcggcata taaagcaatt
     1201 gatttgacaa gtaagttaac acaatctggg tatgaagttc gcgttatgtt aacgaatcac
     1261 gcacaaaaat ttgtgacacc attagcattt caagcaataa gtcgaaatgc tgtttataca
     1321 gatactttta tagaagaaaa tccttcagaa atacagcata ttgcattagg tgattgggca
     1381 gatgcaatca ttgttgcacc tgcaacggca aatacaattg caaaattgag tgtaggtatt
     1441 gctgatgatt tggtgacatc aacgttgcta gcaacagaga caccgaaatt tattgcgcct
     1501 gctatgaatg tgcatatgta tgaaaataaa cgtacgcagc aaaatattaa tattttaaaa
     1561 gaagatgggt atcattttat cgaaccagga agcggatttc tagcatgtgg ttatgttgct
     1621 aaaggacgta tggaagaacc gcttcaaatc gtttctgtaa ttgatgctca ttttcaaaat
     1681 agtaatcgtt tagctaatag ttcatttcaa gataaacgcg cattggttac agcaggacca
     1741 actattgaag ttatcgatcc agtcagattt gtatccaatc gttcttctgg aaaaatgggc
     1801 tatgcaatag ctgaagcatt gcgaaatcga ggagctatcg tgacgttagt tgctggtcct
     1861 acaacactag aggatccaaa agatattgaa gttattcatg ttcaaagtgc tgaagaaatg
     1921 tttgaacaag tgacaagccg atttgacgaa caagatattg ttgtaaaagc agcagccgta
     1981 tctgactata caccggttga tgtattagaa cataagatga aaaagcagga tggtgatttg
     2041 tcagtatctt ttaaacgtac taaagacatt cttaaatatt taggtgaaca taaaacatca
     2101 cagtatttaa taggctttgc agcagagact gaagatattg aaaattatgc acaacaaaaa
     2161 ttacgcaaga aaaatgcaga tgtgattatt tcaaataatg ttggggatat gtctatcgga
     2221 tttagttctg atgataatga attgacaatg cattttaaaa ataatgaaaa agtaaatatc
     2281 aagaaaggaa aaaaagtagt attagctgca caaattttag atgaactaga aactaggtgg
     2341 caataatgat agcgaaagtc atagtcgatg tcgcgtcgaa gagcgttgac tataaatttg
     2401 attatataat tcccgaacaa ctcgaatctg tcatccaacc tggtgtgcgt gtaattgtac
     2461 cttttggacc aagaacgatt caaggttatg taatggaagt aacagcagaa cctgatgcac
     2521 aacttgacgt ttcgaagtta aaaaaaatca tagaagtgaa agatatacaa ccagaattaa
     2581 catcagaatt aatagcttta agtgagtgga tgggttcaac tcatgtcatt aaacgtattt
     2641 ctatgctaga agtgatgctt ccgagtgcta ttaaagcgaa gtataaaaaa gcatttaaga
     2701 tgaaagatga catagagcta ccttcagctt tattacaaaa atttgataag catggttact
     2761 attattataa agatgcgcaa aaaaataatg atattcaatt gcttatgaag ttgttaaaag
     2821 atgatatcgt tgaagaaaaa acgattctca cacaaaatat aactaaaaaa accaagcgtg
     2881 ctgttcgtgt cattgaaggg tatcatcctg atgaagtatt agctaagttg gagaaagtta
     2941 ttaaacaata cgatttgtat gcttacttgt ctgaagaaca acataaaaca atatttttaa
     3001 ctgatattga ggatatgggc ttttcaaaat ccagtttaga tggacttatc aaaaaaggtt
     3061 atgttgaaaa atatgacgcg gttgttgaaa gagacccatt taaagatcgt gttttcgaac
     3121 aagaatcaaa acagcaatta acagaagacc aatataaagc atatgaagcg attaaagcta
     3181 aaattgtaag ccaagagcaa gaaacatttt tacttcatgg tgtgacggga tcaggtaaaa
     3241 cagaagtata tttacaaacg atagaagatg ttttaagcca aggaaaacag gcgatgatgt
     3301 tagttcctga aatcgctcta acaccgcaaa tggttttacg cttcaaacgt cgatttggtg
     3361 atgacgttgc tgtattacat tctggcttat ctaatgggga acgttatgat gaatggcaaa
     3421 aaattaggga tggtcgtgcg agagtaagtg ttggtgcaag gtcaagtgtg ttcgcacctt
     3481 tcaaaaattt agggttaatc atcattgatg aagaacatga atctacatat aaacaagaag
     3541 attatccgag atatcacgct agagaaattg cccaatggcg aagtgaatat catcactgtc
     3601 cagtcatttt aggaagtgca acaccatgtc ttgaaagtta tgcacgagct gaaaaaggcg
     3661 tttatcattt gctatcatta ccaaacagag tgaaccaaca agctttacct gaaattgata
     3721 tagtagacat gcgtgaagaa ttgagtgaag gtaatcggtc aatgttttca aaagatttac
     3781 gtgaagccat acaattaaga ttagatcgac aggaacaagt tgttttattt ttaaatcgac
     3841 gtggttatgc atcgtttatg ttatgtcggg attgtggata tgtaccgcaa tgtccaaact
     3901 gtgatatttc attaacgtat cataaaacga cagacttatt aaaatgtcac tattgtggtt
     3961 accaagagac gccaccgaat caatgtccaa attgtgagag tgaacacatt cgacaagtag
     4021 gtactggtac tcagaaagtt gaagaactat tgcaacaaga atttgaagat gcgcgcataa
     4081 ttaggatgga tgtagataca acctcaaaga aaggtgcaca tgaaaagtta ttgactgaat
     4141 tcgaaaaagg taacggtgac attttactag gtactcagat gattgcgaaa ggattagatt
     4201 atccaaatat tactttagtt ggtgtgctga atgcagatac aatgttaaat ttacctgatt
     4261 ttcgggcgag cgaacgtact tatcaactat taacgcaagt ggctggtaga gctggtcgtc
     4321 atgaaaaggc aggtcaagtc atcattcaaa cgtataatcc agatcattat tcaatattgg
     4381 atgttcaaaa aaatgattat ttaacatttt atcgtcagga aatggaatat cgtaaattag
     4441 gaaagtatcc accgtattat tatttgatta atttcacaat ctcacataaa gaaatgaaga
     4501 aggttatggt agcatcgcag catgttcata aaattttatt acagcattta acagaaaaag
     4561 cgcttgtact aggtccatct ccggcagcac ttgcgagaat caacaatgaa tttagattcc
     4621 aaattttagt gaaatataaa agtgaacctg gattattaca agccattcag tttttagatg
     4681 actattacca tgaaaaattt ataaaagaaa aattagcatt gaagattgat attgatccac
     4741 agatgatgat gtaacattac taattattag aaacaagttc agtatgtacg agtatttgaa
     4801 ccagtgtttg aatatttact ttaagtacag aaaaagggca gaatatacaa ctgttaacta
     4861 tttaaattag cagtttatat tctgtctttt tatatggctt tataacttac gtgattttgg
     4921 tttgataagg aatttattag tattttcatt tacgaattct gattttaatg caagcgccat
     4981 acttactgat gaataaaagt actaatgatt aaacaaacta tttttaaatg g
//
LOCUS       DAKTIO010000176         4968 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.178, whole genome shotgun sequence.
ACCESSION   DAKTIO010000176 DAKTIO010000000
VERSION     DAKTIO010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.178"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            78..1133
                     /locus_tag="O0293_002374"
     CDS             78..1133
                     /locus_tag="O0293_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCY0281236.1"
                     /translation="MTGFSVYLGQPLDEAYIKRMIKQGYQMIFTSVQIPEEDDETKYH
                     YFTKLLNLLKHEQVTYLIDANPSILTPSFYDHLRQYDAQFMIRIDHSTSIEAIEAIMA
                     QGLKCCLNASIISRELLTSLHQQLNDFTLLSFCHNYYPRPDTGLSVDLVNKKNELIYQ
                     FNPKAQIYGFIVGSDLRGPLHKGLPTIEATRHSHPVVAAKLLQETGVSEVLVGDSLIE
                     MRQAKQLIDFCKHRHFTLCIEEVFDTTVTYLFDMCHKVRPDNPENVIRSETSRQICPH
                     SIQPQFTTQRRIGSVTVDNLNNGRYQGEMQIVRQTLSAHDNVNVVAQIIKEDLPLLSC
                     IEPNDTFDFQKTRECKK"
     gene            1133..2029
                     /gene="murQ"
                     /locus_tag="O0293_002375"
     CDS             1133..2029
                     /gene="murQ"
                     /locus_tag="O0293_002375"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCY0281237.1"
                     /translation="MENSTTEARNEATMHLDEMTVEEALITMNKEDQQVPLAVRKAIP
                     QLTKVIKKTIAQYKKGGRLIYIGAGTSGRLGVLDAAECVPTFNTDPHEIIGIIAGGQH
                     AMTMAVEGAEDHKKLAEEDLKNIDLTSKDVVIGIAASGKTPYVIGGLTFANTIGATTV
                     SISCNEHAVISEIAQYPVEVKVGPEVLTGSTRLKSGTAQKLILNMISTITMVGVGKVY
                     DNLMIDVKATNQKLIDRSVRIIQEICAITYDEAMALYQVSEHDVKVATVMGMCGISKE
                     EATRRLLNNGDIVKRAIRDRQP"
     gene            2041..3495
                     /locus_tag="O0293_002376"
     CDS             2041..3495
                     /locus_tag="O0293_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCY0281238.1"
                     /translation="MTKEQQLAERIIAAVGGMDNIDSVMNCMTRVRIKVLDENKVDDQ
                     ELRHIDGVMGVIHDERIQVVVGPGTVNKVANHMAELSGVKLGDPIPHHHNDSEKMDYK
                     SYAADKAKANKEAHKAKQKNGKLNKVLKSIANIFIPLIPAFIGAGLIGGIAAVLSNLM
                     VAGYISGAWITQLITVFNVIKDGMLAYLAIFTGINAAKEFGATPGLGGVIGGTTLLTG
                     IAGKNILMNVFTGEPLQPGQGGIIGVIFAVWILSIVEKRLHKIVPNAIDIIVTPTIAL
                     LIVGLLTIFIFMPLAGFVSDSLVSVVNGIISIGGVFSGFIIGASFLPLVMLGLHHIFT
                     PIHIEMINQSGATYLLPIAAMAGAGQVGAALALWVRCKRNTTLRNTLKGALPVGFLGI
                     GEPLIYGVTLPLGRPFLTACIGGGIGGAVIGGIGHIGAKAIGPSGVSLLPLISDNMYL
                     GYIAGLLAAYAGGFVCTYLFGTTKAMRQTDLLGD"
     gene            3498..4373
                     /locus_tag="O0293_002377"
     CDS             3498..4373
                     /locus_tag="O0293_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCY0281239.1"
                     /translation="MTNILYRIDKQLSDFTKTEKIIADYILKNPHKIIDMTVNDLADV
                     TNVSTASIVRFSRKMTHQGFQELKIAISRYLPEDIATNPHLELIENESVETLKNKMIA
                     RATNTMRFVATNIMDAQIDAICDVLKNARTIFLFGFGASSLTIGDLFQKLSRIGLNVR
                     LLHETHLLVSTFATHDDRDCMIFVTNQGSHSELQSIAQVATHYSIPIITISSTANNPV
                     AQIADYALIYGRTDENEMRMAATTSLFAQLFTVDILYYRFVALNYHAILDCITQSKMA
                     LDNYRKHLATIDFKH"
     gene            complement(4567..4848)
                     /locus_tag="O0293_002378"
     CDS             complement(4567..4848)
                     /locus_tag="O0293_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281240.1"
                     /translation="MVLITYQIILFFIISLSYYLTLNHYMAVTVGNFTSIFGMFAAIL
                     FMYYYLLYKSPEYNQRKRFKHFIHITNLIIIAFSTFVLVHLALKLFFSI"
BASE COUNT         1670 a          730 c         1038 g         1530 t
ORIGIN      
        1 tctcttatag taaaatgtaa gcgattacac aactcaacta tattttccta ataattatat
       61 tgttaaggag ggctactttg acaggctttt cagtgtattt aggacaacct ttagatgaag
      121 cgtatattaa gcgaatgatt aaacaaggtt accaaatgat ttttacatct gtacaaatac
      181 cagaagaaga tgacgagaca aaatatcatt atttcacaaa actactcaat ttattaaaac
      241 atgaacaagt gacttacctc atagatgcta atccatctat tttaacacca tctttttatg
      301 accatcttcg acaatatgat gcacaattta tgattcgtat cgatcatagt acatcaattg
      361 aggcaatcga agcgataatg gcacagggtt taaagtgctg tttgaatgca agtattattt
      421 cccgggaatt gttaacaagc ttacatcaac aattgaatga ttttacatta ctttcatttt
      481 gtcataacta ttatccaaga ccagatacgg gattatctgt tgacttggtc aataagaaaa
      541 atgaactcat ttatcaattt aatccaaagg cacaaatata tggttttatt gtagggagtg
      601 atttgcgagg tcctttgcat aaaggcttgc caacaattga agcaacgaga catagtcatc
      661 ctgtcgttgc agctaaatta ttacaagaaa ctggtgtatc tgaagtgtta gttggagact
      721 cattgattga aatgaggcag gcaaaacaac ttatagattt ttgcaagcat aggcatttca
      781 cgttatgtat tgaagaagtg tttgatacga cagtgactta ccttttcgat atgtgtcata
      841 aagtacgccc ggataatccg gaaaatgtca ttcgttcgga aacgtcaaga caaatatgtc
      901 cacattcgat tcaaccacag tttacgacgc aacgacgcat tggttcagta accgttgata
      961 atttgaataa cggacgttat caaggcgaaa tgcaaattgt gagacaaacg cttagtgcac
     1021 atgacaatgt gaatgttgtt gcacaaatta ttaaagaaga cttaccactg ttaagttgta
     1081 tcgagccgaa tgatacattt gattttcaaa aaactaggga gtgtaagaag tgatggaaaa
     1141 tagtacgacc gaagcgcgta atgaagcgac gatgcatctt gatgaaatga ctgtggaaga
     1201 ggctttaatt acgatgaata aagaagatca gcaagtcccg ttagcagttc gaaaggcaat
     1261 accacaattg acaaaagtaa ttaaaaaaac aattgcacag tataaaaagg gtggacgatt
     1321 gatttatatc ggtgcaggta caagtggaag gttgggtgtc ttagatgcag cggagtgtgt
     1381 acctacattc aatactgacc ctcatgaaat tataggtatt attgctggtg gacaacatgc
     1441 tatgacgatg gctgtagaag gtgcggaaga tcacaaaaaa ttagcggaag aagatttgaa
     1501 aaatatagat ttaacatcaa aagatgtcgt tataggaatt gccgcgagtg gcaaaacgcc
     1561 atatgttata ggcggtttaa catttgctaa cacaatcggt gctacaacag tatctatttc
     1621 atgcaatgaa catgcagtta taagtgaaat tgcgcagtat ccagtagaag ttaaagttgg
     1681 tccagaagta ttaactggtt caacacgttt aaagtctggt acagcacaaa agttaatttt
     1741 aaatatgatt tcaaccatca caatggttgg tgtcggaaaa gtttacgata acctcatgat
     1801 tgatgttaaa gcaaccaatc aaaaactgat cgaccgttca gtgcgtatta ttcaagaaat
     1861 atgtgctatc acatatgatg aagcaatggc gttatatcag gtatctgagc atgatgtgaa
     1921 agttgcgaca gttatgggta tgtgtggcat ttctaaggaa gaagcaacaa gacggttatt
     1981 aaacaatggt gacattgtta aacgagcaat cagagataga caaccttagg agggatttaa
     2041 atgaccaaag aacaacaact tgcagaacga attattgctg cagtaggtgg tatggataat
     2101 atagatagtg tcatgaactg tatgacacgt gtgcgtatta aagtattaga tgagaataaa
     2161 gtagatgacc aagaactaag gcatattgat ggtgtcatgg gtgttataca cgatgaacgc
     2221 attcaagttg tggttggacc tggtacagtc aataaagtgg ctaatcatat ggcggaatta
     2281 agtggtgtta aactaggtga cccaatacca caccatcaca atgatagtga aaaaatggac
     2341 tataaatcat atgcagctga taaagcaaag gcgaataagg aagcgcataa agcaaaacaa
     2401 aagaatggta agttgaataa agtattgaaa tcaattgcca atatctttat accgttgatt
     2461 cctgcattta ttggagctgg attaattggt ggtattgcag cagtactgag taacttaatg
     2521 gtggcaggct atatttcagg tgcttggatt acgcaactta taacagtatt taatgtcatt
     2581 aaagacggta tgttagcata cttagctatt ttcactggta ttaatgcggc taaagaattt
     2641 ggtgcgacac caggacttgg tggcgtgatt ggtggtacaa cgttattaac gggtattgct
     2701 ggtaaaaata ttttaatgaa tgtcttcact ggagaaccat tgcaacctgg acaaggtggg
     2761 attattggcg ttatttttgc cgtttggatt ttaagtattg tcgaaaagag attacataaa
     2821 attgtgccaa atgcgattga tattattgta acgccgacta ttgcattgtt gattgtagga
     2881 ctattaacta tctttatctt tatgccatta gcaggttttg tttcagacag tttagtttca
     2941 gtagttaacg gaattattag tattggtggc gtatttagtg gatttatcat tggtgcaagc
     3001 ttcctaccgt tagttatgtt agggcttcat catattttta cgccaattca tatagaaatg
     3061 attaaccaat caggtgctac ttacttattg ccaattgcag cgatggctgg tgctggacaa
     3121 gtaggtgccg cattagcact ttgggtaaga tgtaaacgca acacaacatt acgtaatact
     3181 ttaaaaggtg cattgccagt tggtttccta ggtatcggag aaccattaat ctatggtgtg
     3241 actttgccat taggtcgacc tttcttaact gcttgtattg gtggtggtat tggtggcgct
     3301 gtaataggtg gaattggaca tattggtgcc aaagcaatag gcccaagtgg tgtgtcacta
     3361 ttaccattaa tctcagataa tatgtattta ggttatattg caggattact tgctgcgtat
     3421 gctggtggat tcgtttgtac atatttattt ggaacgacaa aggcgatgcg acagacagat
     3481 ttgttgggtg attaatgatg acaaatattt tatatcgcat tgataagcag ttgagtgatt
     3541 ttacgaagac agaaaagata atcgctgatt acattttaaa gaatccacat aaaatcattg
     3601 atatgactgt gaatgatttg gcagatgtta cgaatgttag tacagcatca attgttagat
     3661 ttagtcggaa aatgacacat caaggttttc aagagctaaa gattgcgata tctcgatact
     3721 tacccgaaga tattgcaacc aatccacatt tagaattgat tgaaaatgaa tctgtagaaa
     3781 ctttgaaaaa taaaatgatt gctagagcaa cgaatacgat gcgatttgta gctactaata
     3841 ttatggatgc gcaaattgat gcaatttgtg atgtgttgaa aaatgccagg acaatatttt
     3901 tatttggatt tggcgcatcg agtttgacta ttggtgatct ttttcaaaag ttatctcgta
     3961 ttggcttaaa tgtcaggtta ttacatgaaa cgcatttact tgtgtcaaca tttgcgacgc
     4021 atgatgatag agattgcatg atttttgtga cgaatcaagg tagtcatagt gaattgcagt
     4081 caattgcaca ggtggccaca cattacagta ttcccatcat aactatatct agtacagcta
     4141 ataatccagt ggctcaaatt gcagactatg cattgattta tggcagaact gatgaaaatg
     4201 aaatgcgtat ggcggctaca acgtcactat ttgcacagtt attcacggta gatatattgt
     4261 actatcgatt tgtagcatta aattatcatg cgattctaga ttgtataacc caatcgaaaa
     4321 tggcacttga taattacagg aagcatcttg cgacgataga ttttaaacat tagatattgt
     4381 gtgcgttcta acataaagtt agttgcaacg taaacaattg aagtttttga tgtgattcag
     4441 ttgcttgatg aaaaattgtt gctacaagtt tgtaaaactg gcatagaggc tattttgaaa
     4501 atgataaagg cgtgacaaat taaagcaaag ttcagatttg agctttgctt ttttcataga
     4561 tggaaattaa atgctgaaga ataattttaa tgctaaatga actaatacga aggtgctaaa
     4621 agcaattatt atcaaattag tgatatgaat gaaatgttta aatcgtttgc gttgattgta
     4681 ttcgggactt ttatagagta ggtagtagta cataaagagt atggctgcga acatgccgaa
     4741 tattgaagtg aagttaccta cagtgactgc catgtaatga tttaaagtta aatagtaact
     4801 tagactaata ataaaaaata aaatgatttg ataggtaatt aacaccattc aagtccctcc
     4861 attaatcgta gacaaaaaat ttatcaatga tttgattatt tgtattcaaa ttttagtata
     4921 cgacttacct caaagacaaa tattaaaaga taagacaata aaattaga
//
LOCUS       DAKTIO010000177         4955 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.179, whole genome shotgun sequence.
ACCESSION   DAKTIO010000177 DAKTIO010000000
VERSION     DAKTIO010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4955
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.179"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..991)
                     /gene="dinB"
                     /locus_tag="O0293_002379"
     CDS             complement(<1..991)
                     /gene="dinB"
                     /locus_tag="O0293_002379"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCY0281241.1"
                     /translation="MTERRIIHIDMDYFFAQVEMRDNPKLKGKPVIVGGKASSRGVVS
                     TASYEARKYGVHSAMPMSQAHKLCPNGYFVTSNFGAYRETSAQIMSIFRSYTDKVEPM
                     SLDEAYLDITELVRPDLPASKIAQYIRKDILEQTHLTASAGVSYNKFLAKLASGMNKP
                     DGMTVIDYQNVHDILMTLDIGDFPGVGKASKKVMHDNGIFNGRDLYEKTEFELIRLFG
                     KRGRGLYNKARGIDHSEVKSSRVRKSVGTERTFATDVNDDEEILRKVWELSGKTAERL
                     NKLQKSAKTVTVKIKTYQFETLSKQMSLRDSVSSEEDIYNIAYLLYNDLKDPDV"
     gene            complement(1238..1768)
                     /locus_tag="O0293_002380"
     CDS             complement(1238..1768)
                     /locus_tag="O0293_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HCY0281242.1"
                     /translation="MHKIDLTTNNFQMRRFIILQLVIALFVILFTYKWALGVTAVVDQ
                     NIIINLVYGFAGFIILLILHELIHRALFLLFKKDSKPMFNIKKDRILFQTADACFNKW
                     QFSIIMLSPLILLSTGLLILIKVFGYSSLIFMFSMHTAYCFIDILLVALTISSSFKYV
                     QQDEDSIYLYHQKPTQ"
     gene            complement(1938..3299)
                     /gene="rlmD"
                     /locus_tag="O0293_002381"
     CDS             complement(1938..3299)
                     /gene="rlmD"
                     /locus_tag="O0293_002381"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCY0281243.1"
                     /translation="MQAIAKNDIKTGTVVDLTHEGHGVVKIDRFPIFIPQALINEQIE
                     YKIIKVKKNFAIGKLLNINTRSENRVAPPCIYYERCGGCQLQHLSYEAQLEMKKEQVI
                     NLFQRKAHFDNSKINDTVGMTDPWRYRNKSQIPVGKNEQNEVIMGFYRQRSHDIIDME
                     SCLIQDSQHQEVMNEVKSILKDLNVSIYQEQLKKGLMRHLVVRTGYHTDEMMIIFVTN
                     GKKWPQKNAVVEKILDAFPNVTSIKQNINDSHSNVIMGRQSITLYGKDTIIDQLTDST
                     FKISDQSFYQINSEQTEKLYNKAIEYAQLTGNEVVLDTYCGIGTIGLYMAPLAKHVYG
                     VEVVPSAIEDAQQNATINQCNNTTFVCGKAEEVILQWKAQGIKPDVVMVDPPRKGCDE
                     TFIQTLLTLEPKRIVYISCNPATQQRDALLLAEKYQLEEVTPVDMFPQTTHVETVALF
                     NLK"
     gene            complement(3380..4327)
                     /locus_tag="O0293_002382"
     CDS             complement(3380..4327)
                     /locus_tag="O0293_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCY0281244.1"
                     /translation="MRKRARIIYNPTSGKELFKRELPDALIKLEKAGYETSAYATEKI
                     GDATLEAERAMHENYDVLIAAGGDGTLNEVVNGIAEKPNRPKLGVIPMGTVNDFGRAL
                     HIPNDIMGALDVIIEGHSTKVDIGKMNNRYFINLAAGGQLTQVSYETPSKLKSIVGPF
                     AYYIKGFEMLPQMKAVDLRIEYDGNVFQGEALLFFLGLTNSMAGFEKLVPDAKLDDGY
                     FTLIIVEKSNLAELGHIMTLASRGEHTKHPKVIYEKAKAINISSFTDLQLNVDGEYGG
                     KLPANFLNLERHIDVFAPNDIVNEELINNDHVDDNLIEE"
BASE COUNT         1542 a          936 c          685 g         1792 t
ORIGIN      
        1 gtacatcagg gtcttttaaa tcgttataaa gtaaatatgc aatattataa atatcttctt
       61 cagaactaac cgaatctctt aaactcatct gtttagatag cgtttcaaat tgataagttt
      121 taattttaac cgttacagtt ttagctgact tctgtaattt atttagacgt tcagctgttt
      181 tacctgacaa ttcccatact tttcttaaaa tctcttcatc atcattcacg tctgttgcaa
      241 atgtgcgttc agtccctact gattttctta ctcttgatga tttcacttca ctatggtcaa
      301 taccgcgtgc cttgttatat aaaccccgac ctctttttcc aaacaaacgt attaattcaa
      361 attccgtttt ctcatataaa tctctaccgt taaaaatacc attatcatgc attacttttt
      421 tggaagcttt acctacgcct ggaaaatctc caatatccaa tgtcatcaaa atatcatgga
      481 cattttgata atcaatcaca gtcataccat caggtttatt cataccactc gctaatttag
      541 ctaaaaattt gttataagaa acacctgcag atgctgttaa atgtgtctgc tctagaatat
      601 cttttctaat atactgagca attttcgaag caggaaggtc tggtctcact aattctgtaa
      661 tatctaaata cgcttcatcc aatgacatcg gttctacctt atctgtataa cttcggaaaa
      721 tagacataat ctgcgcagat gtttctcggt aagcaccaaa attacttgtg acaaagtatc
      781 catttggaca taatttatgc gcttgtgaca taggcattgc tgaatggacg ccgtattttc
      841 gtgcttcata ggatgccgta gagacaacac ccctactgct tgctttacca ccaacaatga
      901 ctggtttccc tttcaatttg gggttatctc tcatttcgac ttgtgcaaaa aaatagtcca
      961 tatctatatg aataattcgt ctctcagtca agtgctcacc tccctactaa tttttacttt
     1021 tataacgcac aaaaatatct caacataatt atacgctgtg tacgattttt ttacataaat
     1081 cttgcactta gcgataacta tattgagata actacaagtt gttataaaat caattgctat
     1141 ttaagcatga tgatgaagac gattgagtaa gaaaacatag gtaatctgaa ataattcaag
     1201 caaattcatt ttgttggtat catcatatta aaatttatta ttgagtcggc ttttgatgat
     1261 acaaataaat actatcttca tcttgttgaa catatttaaa actactagat attgttaatg
     1321 caacaagcag aatatctata aagcaataag cggtatgcat actaaacata aaaataagtg
     1381 atgaataacc aaatacctta attaaaataa gcaagccagt acttaatagg attagtggtg
     1441 acagcataat aattgagaat tgccatttgt tgaagcaagc atctgctgtt tggaataaga
     1501 ttctgtcttt ttttatatta aacataggtt tgctatcttt tttaaataaa agaaataatg
     1561 ctctatggat aagttcatgt aaaatcaata aaataatgaa tccagcaaac ccatatacaa
     1621 gattgatgat gatattttga tcgacaaccg ctgtgacacc taacgcccac ttatacgtaa
     1681 ataaaatcac gaataacgca ataacaagtt gcaagataat aaaccttcgc atttgaaaat
     1741 tatttgtcgt taaatcaatt ttatgcatta ccaaccctcc cgatcatgac attcttattc
     1801 ttctttaaat atagtataca atgtcacatt taatttaaaa agttcatatc aagaaagtaa
     1861 attggctgta ataaaatttt aatatacgac ttctttcttc acttattaag gcgaaatttt
     1921 atctcaaatc atgtgcgcta tttcaaattg aataatgcca ctgtctcaac atgtgttgtt
     1981 tgtggaaaca tatctaccgg tgttacctct tcaagttgat atttttcagc taataataat
     2041 gcatcacgtt gctgtgttgc gggattacat gaaatataga caatacgctt aggttctaat
     2101 gtaagcaaag tctgaataaa cgtttcgtca cagccctttc ttggcggatc aaccattaca
     2161 acatctggtt taatcccttg tgctttccat tgtaaaataa cttcttcagc tttcccacag
     2221 acaaaagttg tattattgca ttggtttata gtcgcatttt gttgtgcgtc ttcaattgca
     2281 gaaggtacta cttcaacacc gtatacatgt tttgcaagtg gtgccatata tagccctatt
     2341 gttccaatac cacaataggt atctaataca acttcattac ctgtcaattg cgcatactca
     2401 attgctttat tatataattt ctctgtttgt tcagaattaa tttggtagaa tgactgatca
     2461 cttattttaa atgtactatc tgttaattga tcaataattg tatctttacc atatagcgtt
     2521 atagattgac gtcccataat aacattagag tggctatcat taatgttttg tttaatgctt
     2581 gtcacattag gaaatgcatc taatatcttc tcaacaacag catttttttg tggccacttt
     2641 ttaccattag ttacaaaaat aatcatcatt tcgtctgtat gatatcctgt tcttacaacc
     2701 aaatgtctca ttaaaccttt tttcaattgt tcttgataaa tacttacatt taaatctttt
     2761 aaaatagatt taacttcatt catcacttct tgatgttgtg aatcttgtat taaacaactt
     2821 tccatgtcaa taatgtcatg gcttctttga cgataaaagc ccataataac ttcattctgt
     2881 tcattcttac caactggaat ctgggacttg tttcgatatc tccaaggatc tgtcatgcca
     2941 actgtatcgt taatcttaga attatcaaaa tgcgcttttc gctgaaacaa attaatcact
     3001 tgttcctttt tcatttcaag ttgtgcttcg tatgataagt gttgaagttg gcacccacca
     3061 caacgttcat aatatataca tggcggtgca acacgattct cacttctcgt atttatatta
     3121 agcaacttac ctatcgcaaa gttctttttt actttgataa ttttatattc aatttgttca
     3181 ttaattaaag cttgtggtat gaaaatagga aagcgatcta tttttacgac accatggcct
     3241 tcatgcgtta aatcaacaac tgttcccgtt tttatgtcat ttttagctat tgcttgcaaa
     3301 attttacctc caaaatgaac aggttaggaa caaaattatg cgcttcctaa cctgccatta
     3361 tatatttcac tatttctgtt tattcttcga ttaaattgtc atcaacatga tcattattta
     3421 ttaactcttc atttacaata tcattaggtg caaagacatc tatatgacgt tctagattta
     3481 agaaattcgc tggtaattta ccaccatatt ctccatctac atttagttgt aagtctgtga
     3541 atgatgaaat attaattgcc tttgcttttt cataaataac tttaggatgc ttagtatgtt
     3601 ctcctcttga agctaaagtc ataatatgac caagttctgc aaggtttgat ttttcaacta
     3661 taattaacgt aaaatagccg tcatctaact tagcgtccgg cactaatttt tcaaatcctg
     3721 ccattgaatt tgttaaacct aaaaagaata ataatgcttc tccttggaaa acattaccat
     3781 catattcaat tcttaaatct acagctttca tttgaggtaa catttcgaaa cctttgatgt
     3841 aataagcaaa tggaccaaca atagatttca atttactcgg tgtttcataa gagacttgcg
     3901 tcaattgtcc gcctgcagct aaattaataa agtatcgatt attcatttta ccaatatcta
     3961 ctttagtaga atgaccttca atgatgacat caagtgcccc catgatgtca ttaggtatat
     4021 gcaatgcacg tccaaagtca ttaacagtac ccataggaat gacacctagc ttaggacgat
     4081 taggcttttc tgcgatacca ttaactactt catttaatgt tccatcacca cctgcagcga
     4141 ttaatacatc ataattttca tgcatagctc tttctgcttc aagtgtggca tcacctattt
     4201 tctcggttgc atatgcactc gtttcatatc ccgctttttc taattttatt aaggcatcag
     4261 gtaattctct tttaaatagc tctttacctg atgtcgggtt ataaatgatt ctagcacgtt
     4321 tcctcatatc ttatccctct acttaaaatt catatatttt aacttcatct ttgtttcgtc
     4381 taatagggag tgggacagaa ataatattta acaaaattta tttcgttcta ccccaacttg
     4441 cattgtctgt agaatttcct ttcgaaattc tctatgttgg ggccccaccc caacttgcac
     4501 attattgtaa gctgacagaa agtcagcttc tttgtttggg ggccccgcca acttgcacat
     4561 tattgtaagc tgacagaaag tcagcttcta tgttggggcc ccactagaat tgaaaaaagc
     4621 ttgttacaag cgtattttct ttcagtcaac tacagccaat ataacattgt agtgcctagg
     4681 acattgaatt tatgacccag gctcagtctt atttcatcat tcttaatatc gttaaagacc
     4741 aacttgtatc ttaaacaaat actatctcaa tatgtacaaa gcttgttatt tattcagcat
     4801 tttttgccgt tcttcattat atagacttcg tcagttatgc tattttacct ttaaaatgat
     4861 gttgtaaata taatgttgtc tttatacaaa aatgttttat acctttttat tatacagttt
     4921 gccttaatat aattaaagct aacgcattaa taaaa
//
LOCUS       DAKTIO010000178         4952 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.180, whole genome shotgun sequence.
ACCESSION   DAKTIO010000178 DAKTIO010000000
VERSION     DAKTIO010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4952
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.180"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            50..2839
                     /locus_tag="O0293_002383"
     CDS             50..2839
                     /locus_tag="O0293_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCY0281245.1"
                     /translation="MAYQSEYALENEMMNQLEQLGYERVTIRDNKQLLDNFRTILNER
                     HADKLEGNPLTDKEFQRLLTMIDGKSIFESARILRDKLPLRRDDESEIYLSFLDTKSW
                     CKNKFQVTNQVSVEDTYKARYDVTILINGLPLVQVELKRRGIDINEAFNQVKRYRKQN
                     YTGLFRYIQMFIISNGVETRYFSNNDSELLKSHMFYWSDKQNNRINTLQSFAESFMRP
                     CQLAKMISRYMIINETDRILMAMRPYQVYAVEALIQQATETGNNGYVWHTTGSGKTLT
                     SFKASQILSQQDDIKKVIFLVDRKDLDSQTEEEFNKFAKGAVDKTFNTSQLVRQLNDK
                     SLPLIVTTIQKMAKAIQGNAPLLEQYKTNKVVFIIDECHRSQFGDMHRLVKQHFKNAQ
                     YFGFTGTPRFPENSSQDGRTTADIFGRCLHTYLIRDAIHDGNVLGFSVDYINTFKNKA
                     LKAEDNSMVEAIDTEEVWLADKRVELVTRHIINNHDKYTRNRQYSSIFTVQSIHALIK
                     YYETFKRLNKKLEQPLTIAGIFTFKPNEDDRDGEVPYHSREKLEIMISDYNKKFETNF
                     STDTTNEYFNHISKNVKKGVKDSKIDILIVVNMFLTGFDSKVLNTLYVDKNLMYHDLI
                     QAYSRTNRVEKESKPFGKIVNYRDLKKETDDALRVFSQTNDTDTILMRSYEEYKKEFM
                     DAYRELKMIVPTPHMVDDIQDEEELKRFVEAYRLLAKIILRLKAFDEFEFTIDEIGMD
                     EQENEDYKSKYLAVYDQVKRATAEKNKVSILNDIDFEIEMMRNDTINVNYIMNILRQI
                     DLEDKAEQRRNQEQIRRILDHADDPTLRLKRDLIREFIDNVVPSLNKDDDIDQEYVNF
                     ESIKKEAEFKGFAGERSIDEQALKTISNDYQYSGVVNPHHLKKMIGDLPLKEKRKARK
                     AIESFVAETTEKYGV"
     gene            3044..3886
                     /locus_tag="O0293_002384"
     CDS             3044..3886
                     /locus_tag="O0293_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HCY0281246.1"
                     /translation="MDNRNMINRVFSQKILHQIAIKNKSDVVDEAYDFYIQGPKNINV
                     IQKMKSLYNYLKKSYRNEYFYKNTILNKLLLGRHSINTTTALSEMPIGKSIADFILLN
                     GKGVVYEIKTELDKLDRLDNQINDYYEVFNYVVVITNDKHLNKVMARYKDTTVGILVL
                     TTRNTLSEVQKPKENNSLLNTKAMYNFLRKEERKRVIAQNHMDVPTYNDFTEYDVLFD
                     VFKEIPMTKLHNNMISELKKRGNMKEYKDEFLAAPAEIKFLLYFAKMTKKDKNKLYHF
                     LKED"
     gene            3888..4811
                     /locus_tag="O0293_002385"
     CDS             3888..4811
                     /locus_tag="O0293_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HCY0281247.1"
                     /translation="MYYPYLRGKQNELFAIRELLEKGLIGDCIQPIIEPIKYTTTFKN
                     TLQYCGEKAFSINLVVNSKLTEEEISNETVAHLTEIITKNKSVIQKAYLGPSDEGNDR
                     LKQQFSSNSLAILTSVDDWEMFGDKNKLEMVFVPDDRHIKRKLRNIPNKGIIMDPFNK
                     LSRNVDYLDNDDEFYSDDHLYYKEDGYVAFSDYSVIGGEYVDGGFSPLAIAIHIVYFD
                     EANELRVKHFVSDSNNDRSNPGKKFFEAVDKLVTWSKNLDIKNRSYALGQFEELNENN
                     KYPGLGLIKRLSIMHHLEIMNRYLESQNENM"
     gene            4798..>4952
                     /locus_tag="O0293_002386"
     CDS             4798..>4952
                     /locus_tag="O0293_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES domain-containing protein"
                     /protein_id="HCY0281248.1"
                     /translation="MKICEKCFNNTEIVEIIANDNSKFDNCDIDNNHLGVKIFDTTKD
                     IDKLELI"
BASE COUNT         1883 a          629 c          925 g         1515 t
ORIGIN      
        1 agtttatgat atatttagag caatagaaag tgtatggaag gggatatgaa tggcatacca
       61 aagtgaatac gcattagaaa atgaaatgat gaatcaactt gaacaattgg gttacgaaag
      121 agtaacgata cgtgataata agcaattgct tgataatttt agaacgattt taaatgagcg
      181 tcatgcggac aaattagaag gcaatccctt aacagataaa gaatttcaac gtctgttaac
      241 gatgattgat ggaaaaagta ttttcgagag tgcccgtatt ttacgtgata aattaccact
      301 tagacgtgat gatgagtctg agatttattt gtcgttttta gatacgaaaa gttggtgtaa
      361 aaataagttt caagtgacga atcaagtatc tgtcgaggat acatataaag cacgttatga
      421 tgtaacgata ttaatcaacg gactacccct tgtccaagtt gaattgaaac gtcgaggtat
      481 tgatattaat gaggcgttta accaagtaaa acgttaccgc aaacaaaatt acacaggctt
      541 attccgctac atacaaatgt ttatcattag taatggtgtt gaaacgcgat acttttctaa
      601 taatgatagc gaactattga agagtcacat gttttattgg agtgataaac agaataaccg
      661 tatcaataca ttgcaatcgt ttgctgagtc atttatgaga ccttgtcaat tagctaagat
      721 gatatcgcgc tatatgatta ttaatgaaac agatagaata ctgatggcaa tgcgtccgta
      781 tcaagtgtat gcggtagaag cacttattca acaagcgact gagacaggga ataatggata
      841 tgtatggcat acaactggaa gtggtaagac gttgacttct tttaaagcga gtcagatttt
      901 atcacagcaa gatgacatta agaaagttat ctttttggtt gaccgtaaag acttggatag
      961 tcaaacagaa gaggaattta ataaatttgc taagggtgct gtagacaaaa cttttaatac
     1021 ctcgcaactg gtacgccaac taaatgataa aagtttgcca cttattgtaa cgacgattca
     1081 aaaaatggct aaagcgattc aagggaatgc ccctttatta gaacagtata aaacgaataa
     1141 agttgtattt attattgatg agtgtcatcg cagtcaattt ggtgacatgc atcgtctagt
     1201 taaacaacat ttcaaaaatg cccaatactt tggattcact ggtacgccac gttttccaga
     1261 aaatagtagt caagatggta gaacaactgc agatattttc ggtagatgct tacatacgta
     1321 tttaattaga gatgccattc atgatggtaa tgtacttggt ttctcagttg actatattaa
     1381 tacttttaaa aataaagctt taaaagcaga agataacagc atggttgaag caattgatac
     1441 ggaagaagta tggttagcgg ataaacgtgt ggaattagta acacgacata tcatcaataa
     1501 tcatgataaa tatacacgta atcgtcaata ttcaagtata tttacagtcc aaagtattca
     1561 cgcgcttatt aaatattatg agacatttaa gcgacttaac aaaaagttgg aacaaccgtt
     1621 aacgatagct ggtatattta cgtttaaacc taatgaagat gatcgtgatg gtgaagtgcc
     1681 atatcattca cgtgaaaaat tagagataat gattagtgat tataataaaa agttcgagac
     1741 gaatttttca acagacacaa ctaatgagta ttttaatcat atttcaaaaa acgttaaaaa
     1801 gggcgttaaa gatagtaaaa ttgatatctt aatcgttgtt aatatgttct taactggttt
     1861 tgatagtaaa gtactgaaca ctttatatgt tgataagaat ttaatgtatc atgatttaat
     1921 tcaagcgtat tcacgtacaa atagggttga aaaagaatca aagccatttg gtaaaattgt
     1981 aaactatcgt gacttgaaaa aagagacaga cgatgcactg agagtattct cacaaacaaa
     2041 tgatacggat acaattttaa tgcgcagtta tgaagagtat aaaaaagaat ttatggacgc
     2101 ttatcgtgag cttaaaatga ttgtgccgac accacacatg gttgatgaca ttcaagatga
     2161 agaagagcta aagcgctttg ttgaagctta tcgtttatta gctaaaataa tattacgttt
     2221 aaaagcattt gacgagtttg agtttacaat tgatgaaatt ggaatggatg aacaagagaa
     2281 tgaagactat aaaagtaaat atttagctgt gtacgatcaa gtaaaaagag cgacggctga
     2341 gaaaaataaa gtatccattt taaatgatat tgatttcgaa atagaaatga tgcgtaatga
     2401 tacgattaat gtgaattata ttatgaatat attgagacaa attgatcttg aagacaaagc
     2461 ggaacaacgt cgtaaccaag aacaaattag acgcatttta gatcatgcag atgatccgac
     2521 attgaggtta aaacgagatc taattagaga attcatcgac aatgttgtac cttctttaaa
     2581 taaggatgat gatatcgatc aagaatatgt taatttcgaa agtattaaaa aagaagcgga
     2641 gttcaaagga tttgctggag agagatctat cgatgaacaa gccctaaaaa caatttcaaa
     2701 tgactaccag tatagtggtg ttgtaaaccc acatcacctt aaaaaaatga ttggtgattt
     2761 gccattgaaa gaaaagcgta aagcaagaaa agccattgaa tctttcgtgg cagaaacaac
     2821 tgaaaaatac ggtgtgtaat gattcagccc cctcgctaga ttagtgtagg gggcattatt
     2881 attttctatc ttttgttagt taccatgtgt ttggcaacca tcatttggtt tcaatacaat
     2941 tttataatcg tatgtgtata gaaataatgt ctataggcat ttaaaatagt ttataatatt
     3001 aacaggataa gtattaaatg tatgttgaag gaggtgcgta gctatggata atagaaatat
     3061 gattaatcgt gtttttagtc aaaagatatt acatcaaatt gcaatcaaaa ataaaagtga
     3121 tgttgttgat gaggcatatg atttttatat acagggacct aaaaatatca atgtaataca
     3181 gaagatgaaa tctttatata actatcttaa aaagtcttat cgtaacgaat atttttacaa
     3241 aaacacaata cttaataaac tccttctagg acgacattct attaatacaa ctactgcact
     3301 ttctgagatg cccataggga aaagtattgc tgattttata ttgttaaacg gcaaaggcgt
     3361 tgtctatgag attaaaacag aattagataa gctggataga ttagataatc aaattaatga
     3421 ttattatgaa gtgtttaatt atgtcgtagt tattacaaat gacaaacatc tgaataaagt
     3481 tatggctaga tacaaagata caacagttgg aattttagtg ttaacaacta gaaatacact
     3541 gagtgaagtt caaaaaccta aagaaaacaa tagtctctta aacacaaaag cgatgtacaa
     3601 ctttttacga aaagaagaaa gaaaaagagt tattgcacaa aatcatatgg atgtgccaac
     3661 ttataatgat ttcacagagt atgatgtgtt atttgacgtg tttaaagaaa taccaatgac
     3721 gaaactgcat aacaatatga tttctgagtt gaaaaaaaga ggaaacatga aagaatacaa
     3781 agatgaattt ttagcagcgc cggctgaaat taagttcttg ttatatttcg caaaaatgac
     3841 aaagaaagat aaaaataaac tatatcattt tcttaaggag gattaatatg tattatcctt
     3901 atttgcgtgg gaaacaaaat gaactatttg caattagaga attgttagag aaaggtttga
     3961 ttggtgattg tattcaacct ataattgaac caattaaata tacaaccaca tttaaaaata
     4021 ctttgcaata ctgtggtgaa aaagcattct ctataaattt agtagtaaat tcaaagttaa
     4081 ctgaagaaga gattagtaac gaaactgttg cacatttaac tgaaataata acaaaaaaca
     4141 aaagtgttat tcaaaaagct tacttgggtc cttctgatga aggcaatgat aggttgaaac
     4201 agcaattttc aagtaatagt ttagctattt taacaagtgt agatgattgg gaaatgtttg
     4261 gagataaaaa taaacttgaa atggtttttg taccagatga tagacacatt aaacgtaaat
     4321 tgcgtaatat tccaaacaaa ggcatcatta tggatccttt taataaacta agtcgtaatg
     4381 ttgattattt agataatgat gacgagtttt atagcgacga tcacctttat tataaggaag
     4441 atggatacgt agcattttca gactattctg ttataggtgg agaatatgta gacggtggct
     4501 tttcgccatt agctattgcg atacatattg tctattttga tgaggctaat gagctaagag
     4561 ttaagcattt tgtctctgat tctaataatg atagatcaaa tccaggtaaa aagttttttg
     4621 aggctgtaga taaattagta acatggtcaa aaaacttaga tattaaaaat agatcttatg
     4681 cgcttggaca atttgaagaa ttaaatgaaa ataataagta tccaggatta ggtttaatta
     4741 aaaggttatc tatcatgcat cacctagaaa ttatgaatag atacttggag tctcaaaatg
     4801 aaaatatgtg aaaaatgttt taataatact gaaatcgtag aaatcattgc aaatgataat
     4861 agcaaatttg acaattgtga tattgataac aatcatcttg gtgttaaaat atttgatacg
     4921 actaaggaca tagataaatt agaacttatt ag
//
LOCUS       DAKTIO010000179         4929 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.182, whole genome shotgun sequence.
ACCESSION   DAKTIO010000179 DAKTIO010000000
VERSION     DAKTIO010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.182"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            68..1525
                     /locus_tag="O0293_002387"
     CDS             68..1525
                     /locus_tag="O0293_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500488.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCY0281249.1"
                     /translation="MKCLIRFILVLGLLISSAMVYINPTAHAEQDQTWEKIKERGELR
                     VGLSADYAPMEFEHTVNGKTEYAGVDIDLAKKIAKDNNLKLKIVNMSFDSLLGALKTG
                     KIDIIISGMTSTPERKKQVDFSDSYMMTKNIMLVKKDKVNEYKDIKDFNNKKVGAQKG
                     TEQEKIAQTEIENASITSLSRLPDVILALKSGKVEGAVVEKPVAEAYLKQNPKLGISN
                     VKFNEEEKDTVIAVPKDSPKLLSQINKTIKEVKDKGLIDKYMTNAANAMNDDSGFISK
                     YGSFFLKGIKITILISLIGVALGSILGAFVALMKLSKIKIISWIASIYIEILRGTPML
                     VQVFIVFFGITAALGLDISALVCGTIALVINSSAYIAEIIRAGINAVDKGQMEAARSL
                     GLNYRQTMKSVIMPQAIKNILPALGNEFVTLIKESSIVSTIGVGEIMFNAQVVQGISF
                     DPFTPLIVAAALYFVLTFVLTRIMNMIEGRLNASD"
     gene            1518..2240
                     /locus_tag="O0293_002388"
     CDS             1518..2240
                     /locus_tag="O0293_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCY0281250.1"
                     /translation="MIKINNLNKVFGDNEVLKDINLEINQGEVVAIIGPSGSGKSTLL
                     RCMNLLEVPTKGQVIFEGNDLTEKGTQVDKLRQKMGMVFQNFNLFPHKKVVDNIILAP
                     KLLKKDNNDELHKEALSLLDKVGLKEKADVYPNQLSGGQKQRVAIARALAMHPDVILF
                     DEPTSALDPEVVGDVLKVMKDLAKEGMTMVVVTHEMGFAKDVSDKVIFMADGVVVESG
                     TPVEIFEQPQHERTQNFLARVL"
     gene            2391..3518
                     /gene="queG"
                     /locus_tag="O0293_002389"
     CDS             2391..3518
                     /gene="queG"
                     /locus_tag="O0293_002389"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCY0281251.1"
                     /translation="MDTKQLKQDIIDYAYTIGIDSIGFTTADPFDELKQKLEAYHANG
                     YASGFEESDIALRTEPKLSLPTARSIIAIAVGYPNKLKGAPKSVRGDRRGLFARASWG
                     QDYHTIMRKRLDMLAAFIESKVPDVEIKSMVDTGVLSDRAVAERAGLGFVGRNGFVIN
                     PKLGTWTYLGEMLVSIPFEPDDPLLDSCGDCTICVDRCPTSALVGNGQLNSQKCISFL
                     TQTKGYMPDQYRYKIGNRLYGCDTCQQVCPKNRGINTEQDDIILEPEILKPRLVPLLR
                     MSNKEFKQTYGHLAGAWRGKKPIQRNAILALAHFNEVDAIPELKKVATTDERPLIRAT
                     AYWAIGQILGEEARDFINANYDQEDAEVQNEMIKGLDTRRE"
     gene            3523..3993
                     /gene="trmL"
                     /locus_tag="O0293_002390"
     CDS             3523..3993
                     /gene="trmL"
                     /locus_tag="O0293_002390"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCY0281252.1"
                     /translation="MTNHIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKM
                     LKRAGLDYWEFVNITYHDSIEAFFEVTNGEYYLLTKFGKKTYSDFDFSNHDKDFYFIF
                     GKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGLN"
     gene            4152..4751
                     /locus_tag="O0293_002391"
     CDS             4152..4751
                     /locus_tag="O0293_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HCY0281253.1"
                     /translation="MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAP
                     VLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGVPAGQVYPIAYEKDAIELIADKI
                     KTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
                     TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA"
     gene            4776..4928
                     /locus_tag="O0293_002392"
     CDS             4776..4928
                     /locus_tag="O0293_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281254.1"
                     /translation="MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKN
                     SEEDSQ"
BASE COUNT         1835 a          642 c          955 g         1497 t
ORIGIN      
        1 aaacactatt gcatcaaaat gtattaatat gtataattat gaatattaat tagggagggc
       61 tgtataaatg aagtgtttaa ttaggtttat attagtatta gggttgttaa ttagcagtgc
      121 tatggtgtat atcaatccca cagcacatgc tgaacaagat caaacatggg agaagattaa
      181 agaacgcggt gaacttagag tgggtctttc tgcagattat gcaccaatgg aatttgagca
      241 tacagttaat ggtaagactg agtatgcagg tgtagatatt gatttagcta aaaaaattgc
      301 gaaagataat aatttaaaat taaaaatcgt caatatgtca tttgatagtt tgttaggagc
      361 tcttaaaact ggaaaaattg atattattat ttccggaatg acttcaacgc ctgaacgtaa
      421 gaagcaagtt gatttttcag attcatatat gatgactaaa aatatcatgc ttgtaaagaa
      481 agataaagtt aatgaatata aagatatcaa agactttaat aataaaaaag taggggcaca
      541 aaagggaact gaacaagaaa aaatcgctca aaccgaaatt gaaaatgcat ctattacttc
      601 attaagccgt ttaccagacg ttatattagc attgaaaagt ggaaaggttg aaggtgctgt
      661 agttgaaaaa cctgtagcag aagcatattt aaaacaaaat cctaaattag gaatttccaa
      721 tgtgaaattt aatgaagaag aaaaagatac agtgatagca gtgccaaaag attcaccaaa
      781 attattgtca caaattaata aaacgattaa ggaggttaaa gataaaggat taatcgataa
      841 atatatgact aatgctgcaa atgcgatgaa tgatgacagt ggttttattt ctaagtatgg
      901 tagttttttc ttgaaaggaa ttaagattac aatattaatt tcacttatcg gtgttgcatt
      961 aggttctatt ttaggtgcat tcgttgcgtt aatgaaatta agtaaaataa aaattatttc
     1021 atggattgct tctatctata tcgaaatatt aagaggaaca ccaatgttag ttcaagtatt
     1081 tatcgtattc tttggtataa ctgctgcatt aggactagac atttcagctc ttgtttgtgg
     1141 aacaattgca ttagttatta attcctcagc ttacattgct gaaattattc gtgcaggtat
     1201 aaatgctgtt gataaaggcc aaatggaagc cgcacgtagt ctaggtttga attatagaca
     1261 aacgatgaaa agtgtaatta tgccacaagc aattaaaaat attttaccag ctttaggtaa
     1321 tgaatttgtc actttaatta aagaatcatc tattgtgtca acaattggag ttggcgaaat
     1381 tatgtttaat gcacaagtgg ttcaaggtat ttcatttgac ccattcacac cattaatagt
     1441 ggcagcagca ttatactttg tcttaacatt tgtacttaca cgcattatga acatgattga
     1501 agggagattg aatgccagtg attaaaataa acaatcttaa taaagttttt ggagataatg
     1561 aagttttaaa agatatcaat cttgaaatca atcaagggga agtagtagca ataataggtc
     1621 catctggtag tggtaaaagt acattgttaa gatgtatgaa tttattagaa gtacctacta
     1681 aaggtcaagt gatttttgaa ggcaatgact taacggaaaa agggacacaa gtagataaac
     1741 tacgtcaaaa aatgggtatg gtatttcaaa acttcaacct atttccacat aaaaaagttg
     1801 tcgataatat tattttagct cctaaattat taaagaaaga taataacgat gaattacata
     1861 aggaagcatt gtcgttatta gataaagtgg gattaaaaga aaaagcagat gtatatccga
     1921 atcaattatc aggtggtcaa aaacaaaggg tagcaattgc aagagcttta gcaatgcatc
     1981 cagatgttat tttattcgat gaaccaactt cagcattaga tcctgaggta gttggtgatg
     2041 tattaaaagt aatgaaagac ctagccaaag aaggtatgac catggtggtt gtgacacatg
     2101 aaatgggatt tgccaaagat gtaagtgaca aagtcatatt tatggcagat ggcgttgtcg
     2161 tagagtcagg cacaccagtc gaaatatttg aacaaccgca acatgaaaga acacaaaatt
     2221 tcttagcaag agtattataa caacctaacg aagcttgaat atatgatacg caccacaaag
     2281 ttatatcata tattcaagct ttttattatt aaaattatgt gaaatgaaac ctcaatatga
     2341 ataatggttg tgttaaaata acatagtatt attttatggg gggcatacga ttggatacaa
     2401 agcaattaaa gcaagatatc attgactatg catatacaat tggtatcgac agtattggat
     2461 ttactactgc cgatcccttt gatgaattga agcaaaagct agaagcatat catgcaaatg
     2521 gttatgcctc aggatttgaa gaatctgata ttgctttacg aacggagcct aaattatcct
     2581 taccaacagc aaggtcaatc atagcaattg cagttggtta tcctaacaaa ctgaaaggtg
     2641 cacctaagag tgttagaggt gatcgcagag gcttatttgc tagagcatcg tggggtcaag
     2701 attatcatac aattatgcgt aaacgattag acatgttagc tgcatttatt gaatctaaag
     2761 ttccagatgt tgaaatcaaa tctatggtag atacgggtgt attatcagat agggcagtag
     2821 cagaacgtgc aggtttagga tttgttggtc gtaatggctt tgtcatcaat cctaaactag
     2881 gaacatggac atacctcggt gaaatgttag tcagtatacc ttttgaacct gatgatccat
     2941 tattagatag ctgtggtgat tgtacaattt gtgttgatcg ttgtccaaca agtgcattag
     3001 ttggaaatgg ccaattaaat agtcagaaat gcattagttt tttaacgcaa acaaaaggct
     3061 atatgcctga ccaatatcgt tataaaattg ggaatagatt atatggttgc gatacgtgtc
     3121 aacaagtttg tccgaaaaat agaggtatta ataccgaaca agatgacatc attttggaac
     3181 cagaaatttt aaagccaaga ttagtacctt tactacgtat gtctaataaa gaattcaaac
     3241 aaacatatgg tcaccttgca ggtgcttggc gtggtaaaaa gccgatacaa cgaaatgcta
     3301 ttttagcatt ggcgcatttt aatgaagtgg atgcaattcc agaattgaaa aaagttgcaa
     3361 caacagatga aagaccgttg attcgagcaa ctgcttattg ggccattggt caaattcttg
     3421 gggaagaagc aagagatttt atcaatgcta attatgatca agaggatgca gaagttcaga
     3481 atgaaatgat aaaaggatta gacacaagga gagaatagta atatgacaaa tcatatcgtt
     3541 ttatatcaac cagaaatccc agctaatact ggtaacattg cacgtacgtg tgcaggtaca
     3601 aacacacatt tacatttaat taaacctctt ggatttagaa ctgatgataa aatgttaaaa
     3661 agagccggtt tagattattg ggaatttgtg aatattacgt atcatgatag tattgaagca
     3721 ttttttgaag tcacaaatgg cgagtattac ttattaacaa aatttggtaa aaagacttat
     3781 agtgattttg atttttcaaa tcatgacaaa gatttttact tcatttttgg aaaagaaacg
     3841 acaggattac cagactgggt gaaagagaaa tatcaagaca cagcgttaag aattcctatg
     3901 agtgaacata ttcgttcatt aaatttatca aatactgctg cgttgttaat ttatgaagcg
     3961 ttacgtcaac aagattttcc gggattgaat taaaatttac tcattttcat tataaaaatg
     4021 atgcttaagt gctatattta gagtataagc atgttaaagt taaatggttt gatatattga
     4081 aagttagggt ataataatta ttaatatcgc cgtttttatg taaaatgcaa actaagagga
     4141 gtgtagaaat catggcaatg aactttaaag tctttgacaa tagtcaactt gtagcagaat
     4201 atgctgctga tattattaga aagcaattta acaataatcc tactacaatt gcaggttttc
     4261 atttagatac agatcaagcg ccagttctag atgaattaaa gaaaaatgtt gaaaaacatg
     4321 ctgttgattt tagccaaata aatattttag attatgacga taaaaaatca tatttcgaag
     4381 cgttaggtgt accagcaggt caagtttatc caattgctta tgaaaaagat gcaatcgaat
     4441 taatcgctga taagattaaa actaaagaaa ataaagggaa attaacatta caagttgttt
     4501 ctatcgatga gcaaggtaag ttaaatgtta gtattcgtca aggactaatg gaagcaagag
     4561 aaattttctt agtagtgaca ggtgctaata aacgagatgt agttgaaaaa ttatatcaag
     4621 aaaatggtaa aacaagcttc gaaccagccg atttaaaagc acatagaatg gtaaatgtta
     4681 ttcttgataa agaagcggct gcaggtttac ctgaagatgt taaagcttac tttacgtcac
     4741 gctttgctta attaaaaatc tgtgaggtat tgattatgtc taaagacaaa gatccaaaat
     4801 taaattatca tgaagaagaa aacagtatgg taacggattt tgaagattta aaagaattag
     4861 gtaaagaaat ggaacaaatc tctgatcaaa atgatcaaga aaagaattct gaagaagaca
     4921 gtcagtaaa
//
LOCUS       DAKTIO010000180         4867 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.184, whole genome shotgun sequence.
ACCESSION   DAKTIO010000180 DAKTIO010000000
VERSION     DAKTIO010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.184"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            212..2026
                     /locus_tag="O0293_002393"
     CDS             212..2026
                     /locus_tag="O0293_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCY0281255.1"
                     /translation="MNKTKGFTKYKKMRYIPGLDGLRAIAVLGIIIYHLNKQWLTGGF
                     LGVDTFFVISGYLITSLLLKEYDDTGIIKLKSFWIRRLKRLLPAVIVLLMVVGTATLL
                     LKSDNIIRVKHDIIAAIFYVSNWWYIAKDVNYFEQFSFMPLKHLWSLAIEEQFYIFFP
                     VILVTLLLTIKKRYKIGFIFWGVSIISLGLMMFIYSINGDHSRVYFGTDTRLQTLLLG
                     VILAFLWPPFKLKNDPPKVVKYVIDSIGSLSFIVLILLFFIINDETNWIYDGGFYLIS
                     ILTLFIIASVVHPSTWIAKIFSNPVLVFIGKRSYSLYLWHFAVISFVHSYYVDGQIPV
                     YVYFIDISLTIIFAELSYRFIETPFRKEGIKALNWRPSYIPQFIRMAIVVTLLIPFML
                     ILVGAFNKYGKDIIGEKANSFDTTIEDNYLMRIAPIDNIHIDGLVSEKKKESSDVYNN
                     IKPLLIGDSVMVDIGESFKSSVPKSRIDGKVGRQLYQTLPLVKANYSQYKKSSDQVVL
                     ELGTNGDFTVKQLDDLLNQFGKAKIYLVNTRVPRIYEANVNRLLADAAKRKSNVTLID
                     WYKRSQGHSEYFAPDGVHLEYKGVLALKDEILKALKKK"
     gene            2229..4838
                     /gene="clpB"
                     /locus_tag="O0293_002394"
     CDS             2229..4838
                     /gene="clpB"
                     /locus_tag="O0293_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCY0281256.1"
                     /translation="MDINKMTYAVQSALQQAVELSQQHKLQNIEIEAILSAALNESES
                     LYKSILERANIEVDQLNKAYEDKLNTYASVEGDNIQYGQYISQQANQLITKAESYMKE
                     YEDEYISMEHILRSAMDIDQTTKHYINNKVEVIKEIIKKVRGGNHVTSQNPEVNYEAL
                     AKYGRDLVEEVRQGKMDPVIGRDEEIRNTIRILSRKTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVKKDVPESLLDKTVFELDLSALVAGAKYRGEFEERLKAVLKEVKESDGRIILFI
                     DEIHMLVGAGKTDGAMDAGNMLKPMLARGELHCIGATTLNEYREYIEKDSALERRFQK
                     VAVSEPDVEDTISILRGLKERYEVYHGVRIQDRALVAAAELSDRYITDRFLPDKAIDL
                     VDQACATIRTEMGSNPTELDQVNRRVMQLEIEESALKNESDNASKQRLQELQEELANE
                     KEKQAALQSRVESEKEKIANLQEKRAQLDESRQALEDAQTNNNLEKAAELQYGTIPQL
                     EKELRELEDNFQDEQGEDTDRMIREVVTDEEIGDIVSQWTGIPVSKLVETEREKLLHL
                     SDILHKRVVGQDKAVDLVSDAVVRARAGIKDPNRPIGSFLFLGPTGVGKTELAKSLAA
                     SLFDSEKHMIRIDMSEYMEKHAVSRLIGAPPGYIGHDEGGQLTEAVRRNPYSVILLDE
                     VEKAHTDVFNVLLQILDEGRLTDSKGRSVDFKNTIIIMTSNIGSQVLLENVKETGEIT
                     ESTEKAVMTSLNAYFKPEILNRMDDIVLFKPLSIDDMSMIVDKILTQLNIRLLEQRIS
                     IEVSDDAKAWLGQEAYEPQYGARPLKRFVQRQIETPLARMMIKEGFPEGTTIKVNLNS
                     DNNLTFNVEKIHE"
BASE COUNT         1809 a          625 c          921 g         1512 t
ORIGIN      
        1 tttttaagtt gccgagttgc attacaaatt ggatttcgaa gtaagaatca tcattggtaa
       61 tttttttaat ttaacagtat tttagtcaat atgattttga tgatgtcgta attcaataat
      121 atacatatgc atttcaatat tcttttaaac agcataacta aatttaatta agcatctaag
      181 aagaacagtt ttaaaaggga agaatgcaaa aatgaacaaa acaaagggtt ttacaaagta
      241 taagaaaatg agatatattc cagggctcga tggtttgagg gcaatcgctg ttctaggaat
      301 tattatttac cacttaaata agcaatggtt gacaggtggc tttttaggtg tggatacatt
      361 ttttgtgatc tctggttatt taattacaag cttattactc aaagagtatg atgacacagg
      421 tatcattaaa ttgaaaagct tttggatacg tcgtttaaaa cgtttattac cagcagtcat
      481 agttttatta atggttgtag ggacagcaac cttattatta aaatcagata atatcattag
      541 ggttaaacat gatattattg ctgcgatatt ttatgtatca aactggtggt atatagcaaa
      601 agatgttaat tattttgagc aattttcatt tatgccatta aagcatttat ggtctttagc
      661 aattgaagaa cagttttaca tatttttccc agttattttg gttacattat tgttaacaat
      721 taaaaagcga tacaaaatag gatttatttt ttggggagta tcaataattt ctttagggtt
      781 aatgatgttt atctatagta ttaatgggga tcattcacga gtgtattttg gtacagatac
      841 tagattacag acattgttac tgggtgttat tttagctttt ttatggccac cgtttaaatt
      901 gaaaaatgat ccacctaaag ttgtaaaata tgttattgat agcataggta gtttatcatt
      961 tatagtactt atattattat ttttcattat taatgatgag acgaattgga tatatgatgg
     1021 tggtttctat ttaatatcca tattaacgtt atttattatt gctagtgtcg ttcatccatc
     1081 tacatggata gcgaagatat tttcaaatcc agtgttagta tttatcggga aaaggtctta
     1141 tagtttatat ttatggcatt ttgcagtaat tagtttcgta catagttact atgtagacgg
     1201 acagatacct gtatatgtgt actttataga tataagttta acaattatat ttgcagagct
     1261 atcatatcgc tttatagaaa ctccatttag aaaagaaggt attaaagctt taaattggcg
     1321 accttcttat ataccacaat ttataagaat ggcaattgta gtaaccttgt taattccatt
     1381 tatgttgatt ttagtaggtg cattcaataa atatggtaaa gacattattg gagaaaaagc
     1441 gaatagcttt gataccacaa ttgaagataa ttatttaatg cggatagcac caattgataa
     1501 catacacatt gatggcttag taagtgagaa gaaaaaggaa tcttccgacg tatataataa
     1561 tattaaacct cttttaatcg gtgattcagt aatggttgat atcggtgagt catttaagtc
     1621 atcggttcct aagtctagaa ttgatggaaa agtagggcgt caattgtatc aaaccttacc
     1681 tttagttaaa gcgaattatt cacaatataa aaaatcatct gatcaagtcg tattagaatt
     1741 aggtacaaat ggcgacttta ctgtcaaaca gctcgacgat ttacttaatc aatttggaaa
     1801 agccaagatt tatttagtta atacacgtgt tccaagaatt tatgaggcaa atgtaaatcg
     1861 attattagct gacgcggcga aacgaaagtc caatgtcaca ttaattgatt ggtataagcg
     1921 atcacaagga catagtgaat attttgcacc agacggtgta catttagagt acaaaggagt
     1981 cctagcttta aaagatgaaa tattaaaagc acttaaaaag aaataacata tgtttaataa
     2041 gtctagttaa tgtgtaacgt aacattagct agattttttt attcaaaaaa atatttacaa
     2101 atattaggaa atttaagtgt aaaagagttg ataaatgatt atattgggac tataatataa
     2161 ttaaggtcaa agaaagtcaa agtcaaaagt gcaacttgac caactctaca aattagaggt
     2221 gaaattatat ggatataaat aaaatgacat atgctgttca aagtgcttta caacaagcag
     2281 ttgaactgag tcagcaacat aaattacaaa atatagaaat tgaggcaatt ttaagcgctg
     2341 ccttaaatga aagtgaaagc ttatataaaa gtattttaga acgagcaaat attgaggtag
     2401 atcaattaaa caaagcttat gaagacaaac taaacacgta tgcatctgta gaaggtgaca
     2461 atatacaata tggtcaatat attagccaac aagcaaacca attgataact aaggctgaat
     2521 catacatgaa agaatatgaa gatgaatata tttcaatgga gcatatttta cgttcggcaa
     2581 tggacattga tcaaacaaca aaacattata taaataataa agtagaagtt atcaaagaaa
     2641 ttattaaaaa agtaagaggg ggaaatcacg tgacatcaca aaatccagaa gttaattacg
     2701 aagcattagc taaatatggc cgcgacttag tagaagaagt tagacaaggt aaaatggatc
     2761 ctgttatagg aagagatgaa gaaattcgaa atacgattcg tattttaagt cgtaaaacta
     2821 aaaacaaccc tgtgctcatt ggtgaaccag gtgttggtaa aactgcaatt gttgaaggat
     2881 tagcgcaacg tatagttaag aaagatgtgc cagaatcatt attagataaa actgtttttg
     2941 agttagattt aagcgcatta gtagcgggcg ctaaatatcg tggtgaattt gaagagagat
     3001 taaaagcagt cctaaaagaa gttaaagagt ctgatggtag aattatatta tttattgatg
     3061 aaatccatat gcttgtaggt gctggtaaaa cagatggtgc catggatgca ggcaacatgc
     3121 taaaaccaat gttagcacga ggagagttac attgtattgg tgcaacaact ttaaatgaat
     3181 atcgagaata tattgaaaaa gattcggcat tagagcgtcg tttccaaaaa gtagcagtta
     3241 gtgagcctga tgttgaagat acaatttcaa ttttacgtgg tttaaaagaa cgatatgaag
     3301 tgtatcatgg tgtgcgtatt caagatagag ccttagttgc tgccgctgaa ttgtctgatc
     3361 gttacatcac tgatcgtttt ttaccagata aagcgattga tttagttgac caagcatgtg
     3421 caacaattcg tacggaaatg ggatcaaatc caactgaatt ggatcaagtt aatagacgtg
     3481 tcatgcaatt agaaattgaa gaaagcgcac ttaaaaatga atctgacaat gcgagcaaac
     3541 agagattaca agaactacaa gaagagcttg ccaatgaaaa agagaaacaa gcagcacttc
     3601 aatctcgtgt agaatcagaa aaagaaaaaa tagcaaattt acaagaaaaa cgtgcgcaac
     3661 tagatgaaag tagacaagcg ttggaagatg cacaaacaaa taacaattta gaaaaagctg
     3721 ctgaactaca atatggaaca attcctcaat tggaaaaaga acttagagaa ttagaggata
     3781 atttccaaga tgagcaaggt gaagatacag atcgaatgat tcgtgaagtt gtaacagacg
     3841 aagaaattgg cgatattgtc agccaatgga caggcatacc agtttcaaaa ttagttgaaa
     3901 cagaacgtga aaaattactt cacttaagtg acatcttgca taaacgtgtt gtaggtcaag
     3961 ataaagcggt tgacctggtt tcagatgcag tagttagagc aagagcaggt attaaagatc
     4021 caaacagacc tattggtagt ttcttattcc taggtccaac tggagtaggt aaaactgaat
     4081 tagctaaatc attagctgca tcattatttg attctgaaaa acatatgatt cgtattgata
     4141 tgagtgaata tatggaaaaa catgcagtat caagattgat aggggcacct ccaggatata
     4201 ttggacatga tgaagggggt caattaactg aagcggttcg tcgtaatcca tactcagtta
     4261 ttttattaga tgaggttgaa aaagcgcata ctgacgtctt taatgtatta ttgcaaattt
     4321 tagatgaagg ccgtttaact gattctaaag gacgtagcgt tgattttaaa aatactatta
     4381 ttattatgac aagtaatatt ggatctcaag ttttattaga aaacgtaaaa gagactggtg
     4441 aaattacaga atcaacagaa aaagctgtta tgacaagttt aaatgcatat ttcaaaccag
     4501 aaattttgaa tcgtatggat gatatcgtat tatttaaacc attatctatt gatgacatga
     4561 gtatgattgt agataaaatc ttaacgcaat taaatataag attattagaa caacgaatct
     4621 caattgaagt ttctgatgat gctaaagctt ggctaggtca agaagcttat gaacctcaat
     4681 acggtgcaag accattaaaa cgttttgtac aacgccaaat tgaaacacca ttagcacgta
     4741 tgatgattaa agagggattc ccagaaggta caacgattaa agttaattta aattcagaca
     4801 ataacttaac gtttaatgtt gaaaaaattc atgaataaaa ttgaaccaag aatgtgatga
     4861 ttaatcg
//
LOCUS       DAKTIO010000181         4847 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.185, whole genome shotgun sequence.
ACCESSION   DAKTIO010000181 DAKTIO010000000
VERSION     DAKTIO010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.185"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            49..528
                     /locus_tag="O0293_002395"
     CDS             49..528
                     /locus_tag="O0293_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281257.1"
                     /translation="MSDYTKLTKSPKSALLYYYITNGLEFILSVAVYVIFYFIWLRFE
                     WPHYLLYILLVLGALSTLKLFIKPLWQYHCRFYKVDQLSVQYRTSFFIYKEETSRIER
                     LQYLSIKSNPISKVLNLYKVGFMTAGHTIYLPMMSHDDVKIIEERTMSNLRGVESDV"
     gene            521..2104
                     /locus_tag="O0293_002396"
     CDS             521..2104
                     /locus_tag="O0293_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCY0281258.1"
                     /translation="MSEPQKLHPISYFNGIINAIKQNIVVFFIFIIFQLKDFDYTNPE
                     SYLWIGIVFVFFLISYISQIVGIMNTRYWIEDNYFILTTGIFNKKRKELNIKRIQSVD
                     MTQGVVNQIIGGVDLQIKTPSDGIVLSVISKKQGEYLERYIDQLQTELKTEVAQKSSA
                     EKNDNCNHDIEGNVEDIAEGRLDEVQNTDSMLNQKNKRIPIYQMNFKESLFMAMTSGA
                     IGVTLAALVPIYGAVRELIPWSKLNHEISQWVQIVSISVLIIILLVLIVAYIIGTIIT
                     MIRFYGFSVMLENEQLKIEYGLFNKKKVTVPTKRLQAVVERQSYIRRLFGYTAIHFII
                     TSDFENKKIEDDDNDSGSVPILPFIKRDKAYQIIQNLVPDLSYQQVNEGMPLSGFHRH
                     FLIPSIIVIAMAGIGCYFWNQLLLLFTIITIVIIGLFVIKGYLYVKHAGFKMTEEQIT
                     IQNFELLKRNIYYFKAHHIIGFDTWQHTLLKRNNLNNFSFIVAKGVGAQSINLKYARS
                     EDANKLKSWYLRGENDEGL"
     gene            2091..2582
                     /locus_tag="O0293_002397"
     CDS             2091..2582
                     /locus_tag="O0293_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCY0281259.1"
                     /translation="MRDYNFMSPHAKKVMRLSAVLFWIPIALVLLIAFNILNWLFWEI
                     LDNHLSIVSSVIIILLVAVFTLVIAPVYRYKHCRYAFEKHHLRVRNGILFLDEKVIPY
                     FRIQNIDIDVGPIMRRYRLATLTLYTAGGQAEIELIDRSEARRLKRWLNNERHLENNK
                     NEE"
     gene            2586..2945
                     /locus_tag="O0293_002398"
     CDS             2586..2945
                     /locus_tag="O0293_002398"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCY0281260.1"
                     /translation="MIHGIGVDLIEIDRIQALYSKQPKLVERILTKNEQHKFNNFTHE
                     QRKIEFLAGRFATKEAFSKALGTGLGKHVAFNDIDCYNDELGKPKIDYEGFIVHVSIS
                     HTEHYAMSQVVLEKSAF"
     gene            3011..4159
                     /gene="alr"
                     /locus_tag="O0293_002399"
     CDS             3011..4159
                     /gene="alr"
                     /locus_tag="O0293_002399"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCY0281261.1"
                     /translation="MSDKYYRSAYMNVDLNAVASNFKVFSTLHPNKTVMAVVKANAYG
                     LGSVKVARHLMENGATFFAVATLDEAIELRMHGITAKILVLGVLPAKDIDKAIQHRVA
                     LTVPSKQWLKEAIKNISGEQEKKLWLHIKLDTGMGRLGIKDTKTYQEVIEIIQQYEQL
                     VFEGVFTHFACADEPGDMTTEQYQRFKDMVNEAIKPEYIHCQNSAGSLLMDCQFCNAI
                     RPGISLYGYYPSEYVQQKVKVHLKPSVQLIANVVQTKTLQAGESVSYGATYTATDPTT
                     IALLPIGYADGYLRIMQGSFVNVNGHQCEVIGRVCMDQTIVKVPDQVKAGDSVILIDN
                     HRESPQSVEVVAEKQHTINYEVLCNLSRRLPRIYHDGDQRFVTNELLK"
     gene            4244..4414
                     /gene="mazE"
                     /locus_tag="O0293_002400"
     CDS             4244..4414
                     /gene="mazE"
                     /locus_tag="O0293_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCY0281262.1"
                     /translation="MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDC
                     NETYLSSNSTNE"
     gene            4411..4773
                     /locus_tag="O0293_002401"
     CDS             4411..4773
                     /locus_tag="O0293_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCY0281263.1"
                     /translation="MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVA
                     AITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIRTLDKKRLKEKLTYLSDDKMKEV
                     DNALMISLGLNAVAHQKN"
BASE COUNT         1711 a          660 c          932 g         1544 t
ORIGIN      
        1 gaaatagatt ataaatccgt ataatttttt atttggggag aggggtatat gtcagattat
       61 actaagttga ctaagagtcc taagtcagca ttgttgtatt actacattac gaatggctta
      121 gaatttatat tgagtgtagc tgtgtatgtc attttttatt ttatatggtt aagatttgaa
      181 tggccacatt acttacttta tattttgttg gttttaggtg ctctgagtac attaaaatta
      241 tttattaagc ctttatggca atatcattgt cgcttttata aagttgatca actaagtgta
      301 caatatcgta catcattttt tatatataaa gaagaaacga gtcgaattga acgtttacaa
      361 tacttgtcga ttaaatctaa tccaattagt aaagtgctta atttgtataa agttggattt
      421 atgactgcag gtcatactat ttatttaccg atgatgtctc atgacgatgt taaaataatc
      481 gaagagagaa ctatgtcgaa tttgagaggg gttgaaagtg atgtctgaac ctcaaaaatt
      541 acatccgatt tcatatttta atggcataat caatgctatc aaacaaaaca tagtcgtctt
      601 ttttattttc attatctttc aattgaaaga ttttgattat acgaatcccg aatcatattt
      661 atggataggt attgtatttg tattcttttt aatatcatac atttctcaaa ttgtcggaat
      721 tatgaacacg cgatattgga ttgaggataa ttatttcatt ttaacaacag gtatttttaa
      781 taaaaagcgt aaagaattaa atataaaacg tattcagtcc gttgatatga cacaaggggt
      841 cgtcaatcaa attattggtg gtgtggattt acaaatcaaa acaccaagtg atggtattgt
      901 cttaagtgtt atttcgaaaa aacaaggtga atatcttgaa cgatatattg atcaattgca
      961 aacagagttg aagacagaag tggcgcaaaa gtctagtgct gagaagaatg acaactgtaa
     1021 tcatgacatt gaaggtaatg tggaagacat cgcagaaggt cgcttagatg aagtgcagaa
     1081 cactgatagt atgcttaatc agaaaaataa acgcattcct atatatcaga tgaattttaa
     1141 agaatcacta tttatggcaa tgaccagtgg cgccattggt gtaacgttag cagctttagt
     1201 accgatttac ggggctgtaa gagagttgat cccatggtcg aagctaaatc atgaaattag
     1261 tcaatgggtt caaattgtaa gtatttcagt attaatcatc attttactag tattaatagt
     1321 ggcatatatt attggaacga ttataacaat gataagattt tatggctttt cggtgatgct
     1381 tgaaaatgag caacttaaaa ttgaatatgg tctctttaac aagaagaaag taactgtacc
     1441 aacgaaacga ttgcaagctg ttgttgaacg tcagtcatat atacgaagat tatttggcta
     1501 cacagcaata cattttatca tcacaagtga ttttgaaaat aaaaaaatcg aagatgatga
     1561 caatgattca ggtagtgtgc cgatattgcc gtttattaaa cgagacaaag catatcaaat
     1621 tatccagaat ttagtaccag atttatctta tcaacaagtt aatgaaggta tgcctttgtc
     1681 aggtttccac agacactttt taatacctag tattattgtc atagctatgg caggtatagg
     1741 ctgttatttt tggaatcaat tattattgtt atttacgatt ataacaatag tcattattgg
     1801 tttatttgtg atcaaaggat atctttatgt caaacatgcg ggttttaaaa tgacagaaga
     1861 acaaattact atccagaatt tcgagctatt gaaacgaaat atttattatt ttaaagcgca
     1921 tcacattata ggatttgaca cttggcaaca tacattactt aaacgcaaca atttaaataa
     1981 ttttagcttt attgttgcta aaggtgtggg agcccaatcg attaacttaa aatacgcaag
     2041 aagtgaagat gcaaataaat tgaaatcatg gtatttaaga ggtgaaaatg atgagggatt
     2101 ataattttat gtcaccacac gctaaaaaag tgatgaggtt aagtgctgtg ctgttttgga
     2161 tacccatagc gcttgtactt ttaatcgcat ttaatatttt gaactggcta ttttgggaga
     2221 ttttagataa ccatttatcc attgttagta gtgtgattat aatattactt gttgctgttt
     2281 tcacgcttgt tatagcgcca gtctatcgat ataagcactg cagatatgca tttgaaaaac
     2341 accatttgcg tgtacgaaac ggcattttat ttttggatga aaaggtaata ccatattttc
     2401 gtattcaaaa tattgatatc gatgttggtc ctataatgag acgctatcga ttagcaactt
     2461 tgacccttta tacagcgggt ggtcaagctg aaattgaatt aatagataga agtgaagcac
     2521 gtagattgaa acgatggtta aataacgaga gacatttaga aaacaataaa aatgaggaat
     2581 aatcaatgat acatggaatt ggtgtagatt taatcgaaat cgatcgaata caagcgttat
     2641 atagtaagca accaaaattg gttgagcgga ttttaactaa aaatgaacag cacaaattca
     2701 acaatttcac acatgagcaa cgtaaaattg aatttttagc tggcaggttt gctacaaaag
     2761 aagcgttcag taaagcatta ggcacaggct taggaaaaca tgtagctttt aacgatatag
     2821 actgttacaa cgacgaactt ggcaaaccaa agattgatta cgaagggttt atcgtacatg
     2881 ttagtatctc acacactgag cattatgcga tgagccaagt tgttttagaa aagtcagcat
     2941 tttaaatttg ttatacaatg ataattaaga tctagtttta aaacgtgtgg gaattacagg
     3001 aggaagtaat atgtcggata aatattatag atctgcgtat atgaatgtag atttaaacgc
     3061 tgttgcatca aatttcaaag tattcagtac attgcatcca aataaaacag tgatggctgt
     3121 cgttaaagcc aatgcctatg gactaggtag tgttaaagta gcacgtcatt taatggaaaa
     3181 tggcgccaca ttttttgctg tagcaacgtt agatgaagcg atagaactta gaatgcatgg
     3241 gattactgct aaaattttag tcttaggtgt gttaccagct aaagatattg ataaagcgat
     3301 acaacaccga gttgccttaa cggttccgtc taaacagtgg ttgaaagaag caattaaaaa
     3361 catttctggt gagcaagaga aaaagttatg gttgcacatt aaattagata caggaatggg
     3421 acgtttaggt attaaagata ctaaaacgta tcaagaagtg attgaaatca ttcaacaata
     3481 tgagcaactt gtatttgaag gcgtgtttac acactttgcc tgtgctgacg aaccaggaga
     3541 tatgacaact gaacaatatc aacgttttaa agatatggtc aatgaagcaa ttaaacctga
     3601 atatatacat tgtcagaact cagcaggctc tctattaatg gattgccaat tctgtaatgc
     3661 aataagacca ggaatttccc tttatggata ttatccatca gagtatgtac agcaaaaagt
     3721 taaagtacac cttaaaccaa gtgtgcaatt aattgctaat gtagttcaaa caaagacgct
     3781 acaagcgggt gagtctgtaa gttatggtgc aacttataca gctactgacc caactacaat
     3841 agcattgtta cctattggat atgcagatgg ctatttacgc ataatgcaag gtagctttgt
     3901 aaatgtaaat ggtcatcaat gcgaagttat tggtcgcgta tgtatggatc agacaattgt
     3961 taaagtgcca gatcaagtta aagctggaga ttcggtgatt ttaatagata atcatagaga
     4021 aagtccacag tcggtagagg tggtagctga aaagcaacat actattaatt atgaagtgct
     4081 ttgtaacttg tcgagacgtt tgccgcgaat ctatcatgat ggtgatcaac gttttgtaac
     4141 aaatgaattg ttaaaataat atggtcagtg catataaaat ttgttatcat tagagtaatt
     4201 aaaggtcatt taataacttt tggaatcaat tggaggttct catatgttat cttttagtca
     4261 aaatagaagt catagcttag aacaatcttt aaaagaagga tattcacaaa tggctgattt
     4321 aaatctctcc ctagcgaacg aagcttttcc gatagagtgt gaagcatgcg attgcaacga
     4381 aacatattta tcttctaatt caacgaatga atgattagac gaggagatgt ttatttagca
     4441 gatttatcac cagtacaggg atctgaacaa gggggagtca gacctgtagt cataattcaa
     4501 aatgatactg gtaataaata tagtcctaca gttattgttg cggcaataac tggtaggatt
     4561 aataaagcga aaataccgac acatgtagag attgaaaaga aaaagtataa gttggataaa
     4621 gactcagtta tattattaga acaaattcgt acacttgata aaaaacgatt gaaagaaaaa
     4681 ctgacgtact tatccgatga taaaatgaaa gaagtagata atgcactaat gattagttta
     4741 gggctgaatg cagtagctca ccagaaaaat taggcgtcta ttatatgtat ttttcagaga
     4801 taaataaaat attgatataa aagacaataa ctttataata attataa
//
LOCUS       DAKTIO010000182         4796 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.186, whole genome shotgun sequence.
ACCESSION   DAKTIO010000182 DAKTIO010000000
VERSION     DAKTIO010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.186"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..1152)
                     /locus_tag="O0293_002402"
     CDS             complement(<1..1152)
                     /locus_tag="O0293_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCY0281264.1"
                     /translation="MKTYDLIVIGFGKAGKTLAKYAASTGQHVAVIEQSPKMYGGTCI
                     NIGCIPSKTLVHDGLEGKSFEASYNRKNDVVNALNNKNYHLLADDNNIDVLDFKAQFK
                     SNTEVNLLDQHDDIVDSITAPHIIINTGATSVIPNIKGLDQAKHVFDSTGLLNISYQP
                     KHLVIVGGGYIALEFASMFANLGSKVTVLERGESFMPREDQDVVAYGITDLENKGIAL
                     HTNVETTELSSDNHHTTVHTNVDNFEADAVLLAIGRKPNTDLALENTDIELGDRGEIK
                     VNAHLQTTVPHIYAAGDVKGGLQFTYISLDDYRIIKSALYGNQSRTTDNRGSVPYTVF
                     IDPPLSRVGLTSKEAAAQHYDYTEHQLLVSAIPRHKINNDPRGLFKVVIN"
     gene            complement(1139..1579)
                     /locus_tag="O0293_002403"
     CDS             complement(1139..1579)
                     /locus_tag="O0293_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCY0281265.1"
                     /translation="MNLEFNIAVHVLAFLTKHHSEKFNSSSLAELTCLNPVQLRRVTT
                     QLVDLKMIDTIRGKDGGYLANDQSADVSLATLYKHFVLEKEHHTRLFTGDEGSHCQIA
                     RNIATTMSHYQQDEQNIIINFYNGKTIKDVIEDIQKEDLCHENI"
     gene            1637..1738
                     /locus_tag="O0293_002404"
     CDS             1637..1738
                     /locus_tag="O0293_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281266.1"
                     /translation="MCQYLCLKSVELGVVIVMIYCLKVDALTVRLHN"
     gene            2204..3613
                     /locus_tag="O0293_002405"
     CDS             2204..3613
                     /locus_tag="O0293_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCY0281267.1"
                     /translation="MSIDMYLDRSRNQASSVGNLSQTMNSNYDALEKAITQFINDDAL
                     KGKAYTSAKQFFSTVLIPLSTSMKTLSDLTKQACDNFVSRYTSEVDSISLKESELEED
                     IRSLSQQITRYENLNNNLKKHASDNQQAISSNQQIIRTLGQQKHELEEKLRKLREFNQ
                     KSPEIFKEVEEFQKIVQQGLTQAQNFWNFSTNQFNIPSGKELDWAKASHEKYLKVAMG
                     KIEHKAEKETLNKADFAVIKAYAKEHPEDDIPKSILKYINDNKDSIKRDIGLDITSTL
                     LEQGGINASKFGVFINTAGGVKGPAGPNSFVEVKRTSGNVFIENGSKFAKGGKYLGKG
                     VAGVGFGIGMYDDLANDDKTFGEALSHNGMTLAAGSAGTAVGAGLATFVLGSNPVGWV
                     ILAGLAMSTVFALGTDLIYQNNIFGLKDKVDWVGHKIDNSIDVVKKTTEKSMDSVGNA
                     VSEAKNIISNHINPMKWAW"
     gene            3617..4243
                     /locus_tag="O0293_002406"
     CDS             3617..4243
                     /locus_tag="O0293_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0281268.1"
                     /translation="MLFNIKVINKNPRYKVVQYNDEYLLIDLVSTWLVYFFPFINWFI
                     PKRYAKLSEKELENLNVDKQNKNNIFWPVTGSSFLFVVILRKYVHTFEVQLDNKILIS
                     LCFIGFIGIAAFYIYLNKKLKLKIYDDNLDNENRVILVPTFKDGSFIVFTYLLLGGCS
                     ILFLIWLMTIKPQNLLVFIMWIIITIFFFLISMGSISNKKVYAKLKKQ"
     gene            4369..>4796
                     /locus_tag="O0293_002407"
     CDS             4369..>4796
                     /locus_tag="O0293_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0281269.1"
                     /translation="MEILLCEVRVVNKNPRYRIIKYKNDYLMIDLVSTWLVLFFPFIN
                     WLIPKKYVQISREDFDNLNIVKPVKNKALWPAIGSILLFGTMFRDKIYIPDSHLEKNC
                     VIIICSVLLLSILVFYIYLNQKVKLSIYNDRSSNGKIMIFP"
BASE COUNT         1673 a          647 c          858 g         1618 t
ORIGIN      
        1 attaatgact actttaaata aacctcttgg atcattgtta attttatgac gaggtatagc
       61 acttactaaa agttgatgtt cagtgtaatc ataatgttga gcggcagctt ctttactagt
      121 taatccaaca cgtgataatg gtggatctat aaatactgta taaggcacgc tgcctctatt
      181 gtcagtcgta cgtgactgat taccatataa cgctgatttg ataattcgat aatcatctaa
      241 agatatatac gtaaattgaa gtccgccttt aacatcacct gcagcataaa tatgcggcac
      301 agttgtttga agatgagcat tgactttaat ttcgcctctg tcgcctaatt cgatatcagt
      361 attttctaaa gctaaatccg tattcggttt gcgcccgata gccaaaagta ctgcatcagc
      421 ctcaaagtta tcaacgttgg tatggactgt tgtatgatga ttgtcagatg acaattcagt
      481 cgtttcaaca tttgtatgca atgcaatgcc tttattttct aagtcagtaa taccatatgc
      541 aacgacatct tgatcttcgc gtggcataaa tgattcgcca cgttctaata ctgttacctt
      601 actacctaaa ttcgcaaaca ttgaagcaaa ttctaaggcg atataaccgc cacctacaat
      661 aacgaggtgc ttaggttgat agctaatgtt taataaacct gtcgaatcga agacgtgttt
      721 agcttgatca aggcctttaa tgttaggaat gacagaggta gcaccggtat taataatgat
      781 atgaggtgca gtaatactat cgacgatatc gtcatgttga tctaataaat tcacttcagt
      841 attagattta aactgcgctt taaaatccag tacatcaatg ttgttatcgt ctgctaataa
      901 gtggtaattt ttattgttta gcgcattgac aacatcgttt ttacggttat aacttgcttc
      961 aaaagatttg ccttctaatc catcatgtac aagtgtcttc gaaggtatac atcctatgtt
     1021 tatacaagtg cctccataca ttttcggaga ttgttcgata actgcgacgt gttgacctgt
     1081 tgatgcagcg tatttcgcta aagttttacc agctttccca aatcctatta caattaaatc
     1141 atatgttttc atgacataaa tcctcctttt gaatgtcttc aatgacatct ttgattgttt
     1201 ttccattata aaaattaata atgatattct gttcgtcttg ctgataatgt gacatggtag
     1261 ttgcaatatt acgagcaatt tgacagtgac tgccttcgtc gccagtaaat agacgtgtgt
     1321 ggtgttcttt ctctaagaca aaatgtttat ataatgttgc tagagagaca tcagcacttt
     1381 gatcatttgc taaataaccg ccatctttac ctcgtattgt gtcaatcatt tttaaatcga
     1441 caagttgagt cgtcacgcgt cgtaattgaa caggatttaa acaagttaat tctgctaatg
     1501 aactactatt gaatttttct gaatgatgct tagttaaaaa agctaataca tgcacggcaa
     1561 tgttaaattc taaattcaat gtttctcacc tcataattgt aactgtatta gttacaatta
     1621 aaaatcattt tgctcaatgt gtcaatactt atgtttgaaa agtgttgaat taggtgtggt
     1681 tattgtgatg atttattgtt tgaaagttga tgcattaacg gtgagattgc acaattgaaa
     1741 ttttaattat gtatgaactg atagatatga aatgaatatt gaaaatgaga gcaataaaat
     1801 tttgaatgat gatgttgtgt gattgtataa acttattcgt attttagata cgttttgata
     1861 tttcgcatga tgaggaaata cttttaacaa ctgatgacat attttaatag tgaatatatt
     1921 ttgagatatt aatatattta atgttaagga tgttgtcgca ataaaattaa aatattgaaa
     1981 gataattttt attgattaat tgcgaatata caaagacacc attatatcca tatgctaaaa
     2041 taagaaaaaa ggcgacatgt tatctcaatt tacctgcatc tagactcctt tacataagaa
     2101 ataatcatcg ccgagtttta aaattttaca actttgtaga tgtaaaaagc atttgatttt
     2161 aaatagtttg aaaatcatat atttttgaaa aaggagcatg ccgatgagta ttgacatgta
     2221 tttagacaga tctcgaaacc aagcttcaag tgtggggaat ttgagtcaaa caatgaattc
     2281 aaattatgat gcgttggaaa aagcaattac tcaatttatt aatgatgatg cgcttaaagg
     2341 gaaagcgtat acgtcagcta agcaattttt tagtacggtg ttaattccat tatcaacaag
     2401 tatgaaaaca ttgagtgatt taacgaagca agcttgcgat aattttgtgt cacgttatac
     2461 gagtgaggtt gatagcatat ctttaaaaga atcagagctt gaagaagata tcagatcatt
     2521 aagtcaacaa attacgcgat atgaaaattt gaataacaat ttgaaaaagc atgcttccga
     2581 taatcagcaa gccatttcat cgaaccaaca aataatacga acattaggtc aacaaaaaca
     2641 tgaattagaa gagaagctac gcaaattgcg tgagtttaat caaaaatcac cagaaatatt
     2701 taaagaagtt gaagaatttc aaaaaattgt ccaacaagga cttacccaag cgcagaattt
     2761 ttggaacttt tcaacaaatc aatttaatat tccttcaggt aaagaacttg attgggccaa
     2821 agcaagtcat gaaaaatatt tgaaagttgc tatggggaaa attgaacata aagcagagaa
     2881 agaaacttta aataaagcag actttgctgt tataaaggca tatgccaaag aacatccaga
     2941 agacgatatc ccgaaaagta tattgaaata tataaatgac aataaagaca gtattaaaag
     3001 agatatagga ttagatatta cgtcaacact tttagagcaa ggcggtataa atgcaagtaa
     3061 attcggtgta tttatcaata cagcaggtgg agtgaaaggc ccagcaggtc caaattcatt
     3121 tgtggaagtc aaacgtacat caggtaatgt gtttatagaa aatggtagta aatttgcaaa
     3181 aggcggaaaa tacctaggta aaggtgttgc tggtgtagga tttggtatag gtatgtatga
     3241 tgaccttgca aatgatgata aaacatttgg agaggcgttg tcgcataatg gtatgacgct
     3301 tgcagctgga tctgcaggga cagcagttgg agctggatta gcgacttttg ttttaggaag
     3361 taatccagta ggatgggtga ttttagcagg tttggctatg agtacagtat ttgcattagg
     3421 aacagattta atttatcaaa ataatatttt tggattaaaa gataaagtag actgggtagg
     3481 gcataaaatc gataatagta tagatgttgt taaaaaaact acagaaaaat ccatggatag
     3541 tgttgggaat gctgttagtg aagctaaaaa tattattagc aatcatataa atccaatgaa
     3601 atgggcgtgg tgagttttgc tttttaatat taaggttatt aataaaaatc cgagatataa
     3661 agtcgttcaa tataatgatg agtatttgtt aattgattta gtaagcactt ggcttgtata
     3721 cttttttcct tttattaatt ggttcattcc caaaaggtat gcaaaactta gtgagaagga
     3781 acttgaaaat ttaaatgttg ataaacaaaa taaaaataat attttctggc cagttactgg
     3841 tagttcgttt ttatttgtag ttattttacg gaaatatgta catacgtttg aagttcaatt
     3901 agataataag attttaatat ctttatgttt tataggattt ataggtattg cagcatttta
     3961 catttactta aataaaaagc ttaaactaaa gatatatgat gataatctag ataatgaaaa
     4021 tagggttata ttagtgccta cttttaaaga tggaagtttt atagttttca catatctatt
     4081 attaggaggt tgttctatat tatttttaat atggctaatg actataaaac ctcaaaatct
     4141 actggtattt attatgtgga ttattattac aatttttttc tttctaataa gcatgggctc
     4201 aattagtaat aaaaaggttt atgccaaact taaaaagcaa tagaaattac taaacttaga
     4261 ttgtagttcg taagttaagt aataaacagg aaaatagtat gttactaaaa tacaaaacac
     4321 ttataaagta tctgaaagtg tattagatat aaaaccaacg agacggaagt ggagatattg
     4381 ctttgcgagg ttagagttgt aaacaaaaat cccagatata gaattattaa atataaaaac
     4441 gactatctga tgattgattt agtaagcacc tggttagttt tgttctttcc gtttattaat
     4501 tggttaatac caaaaaaata tgtccaaatc agcagagaag attttgataa tttaaatatt
     4561 gtcaaaccag ttaaaaataa agctttatgg ccagctatag gtagcatact tttatttggc
     4621 actatgttta gagataaaat atatatacct gattctcatt tagaaaaaaa ttgcgttatt
     4681 atcatttgtt cggttttatt attaagtatt ctagtgtttt atatatattt aaatcagaaa
     4741 gtaaagttat ctatctataa tgatcggagt agcaatggaa aaattatgat ttttcc
//
LOCUS       DAKTIO010000183         4768 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.187, whole genome shotgun sequence.
ACCESSION   DAKTIO010000183 DAKTIO010000000
VERSION     DAKTIO010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.187"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            547..1587
                     /locus_tag="O0293_002408"
     CDS             547..1587
                     /locus_tag="O0293_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HCY0281270.1"
                     /translation="MQQVTSDIMTFRGSHFELGVKTGKWLQQTPLLKNREKEWKKRVP
                     RFDIDVNETYQIFQTYAPQIWEELMGLQSILKMPTRQIILNFGHYRFTDLKESGCTVF
                     QGKDFMVRNYDYHPATYDGRYLLYQPTDSGLAQIGPVSRVTGRMDGMNESGLTMGYNF
                     MHRKKPANGFVCYMIGRLILENCRNVTEAIQLLKEIPHRSSFSYILMDKSLNHAIVEV
                     TPRSIDVRYDNICTNHFEILTHENRNYTKESKERLARTISQTNDNLDMTTAFKLFNNP
                     QYEIYSKLFKSWSGTIHTSMYQPETLTAYFTLGENAPPEIIDFKSWLDGQDLNITHFT
                     GNIDTDLTFANK"
     gene            complement(1644..2486)
                     /locus_tag="O0293_002409"
     CDS             complement(1644..2486)
                     /locus_tag="O0293_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HCY0281271.1"
                     /translation="MKKFFFIGLLVFVVFFTAATIIWFSYDKNKYGTKQYDKTFKDDA
                     FDNVSINLDSTELRIKRGNQFRVKYDGDNDILINIVDKTLKISDKRSKTRGYAIDMNP
                     FHENKKTLTIEMPDKMIKRLNLSSGAGSVRISDVDLENTSIQSINGEVVIKNSNLDAL
                     DSKTNNSSTYISKSNIKNSNIKVVIGTLQIDKSQIKQSIFLNDHGDIEFKNMPSKVDA
                     KASTKQGDIRFKYDSKPEDTILKLNPGTGDSVVKNKTFTNGKVGKSDNVLEFYTIDGN
                     IKVE"
     gene            complement(2483..3040)
                     /locus_tag="O0293_002410"
     CDS             complement(2483..3040)
                     /locus_tag="O0293_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001669025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HCY0281272.1"
                     /translation="MNKITYLNELETALDALPRHLRDQKMYEYERYFYEQELNGVDEG
                     EILKKLKDPQDVAAETKARSVIDYAESKPTFENISRAVAASLSLGILSIFVILIPVSI
                     VGLFVLALFLISLLLLFCPIILLASAISRGIVDSISNVFFAISYSGLGLVFIIVIFKI
                     LEYIYRLILKYLLWYIKTVKGSVRK"
     gene            complement(3100..3624)
                     /locus_tag="O0293_002411"
     CDS             complement(3100..3624)
                     /locus_tag="O0293_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281273.1"
                     /translation="MLYLNKYKERVTSVRIQNRWVVFILFLICSFGVLIGLYQYRHTK
                     TVDLSNLEINDIKLNEKFDKKGYEVNKKIKFDRFKFYNSKAHPDLTVKVREKDNIVKG
                     IILVRDEKIHTNFDGGIGSPINNAIENLGFGYKRTKVGNDFSSVKYIDRDNHLKLNLL
                     YQDLEIKRIEFFSK"
     gene            3745..4308
                     /locus_tag="O0293_002412"
     CDS             3745..4308
                     /locus_tag="O0293_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCY0281274.1"
                     /translation="MTNLETYFKNSQPLNEYIDGMKTNQDTVLSIYQSFELPEDDSRI
                     KKIKEMNYSKVLVITEDWCGDAMMNLPILKHISEALNLEVRVFHRDDDTKLIDQYLTN
                     GKSRAIPIFVFLNDQFEQETVWGPRANEVQKFVTDVRADKLPSKDHPDYADLEKETHL
                     VISNRYKTDASFWKAVYNSILNKLENK"
     gene            complement(4374..>4768)
                     /gene="pmtD"
                     /locus_tag="O0293_002413"
     CDS             complement(4374..>4768)
                     /gene="pmtD"
                     /locus_tag="O0293_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtD"
                     /protein_id="HCY0281275.1"
                     /translation="VILIVVQASSNGKNLAFSKYVDNLWFFLIFLLFFGLFLFLITLA
                     SQKTAMIFSLGVFLVLIVPFIKPFITFIPRYGEKVLDAFDYIPFAYLTDKMISSNFDF
                     SNWQWVISLGSIVIFFILNILYVAKKDI"
BASE COUNT         1750 a          805 c          620 g         1593 t
ORIGIN      
        1 ataagcaaat gctactacac caacaccagc aactaatgtt gtaatagcaa ctaagctaaa
       61 tacgaattta aacacacatt taataaaatt ccacatctca atttcacctc tgttaaatta
      121 aatacctatc tacatttcat tatactatca tatatagaat aatcattaaa taaataccca
      181 ctttcacgaa atatattcat caaattatat agtgagcaag ctaatgatta ttttcaaaaa
      241 tgccgaggaa cctctgtttc aacgtgacat gaccttatat gctaatgtta acaatgacta
      301 ttatcgtgaa tgctaactta agattgatat ttatgataag atgattttaa gtttttaact
      361 caatatcccc accatctata aaaatatgtt ttaactgatt gataagtctt atacaccgag
      421 aaatcacctc aaacaatttt atatttcatt tagtaaaata taactttatt taacatgaca
      481 ttcaatatac atacgattac tatttattta caatatatta caactaaaaa gaaaggggcc
      541 gatgatatgc aacaagtgac atcagatatc atgactttta gaggttcaca tttcgagtta
      601 ggtgttaaaa ctggtaaatg gcttcaacaa acacctcttc taaaaaatcg agaaaaagaa
      661 tggaaaaaac gtgtcccacg tttcgatata gatgtaaatg aaacttatca aatattccaa
      721 acatacgcac ctcaaatatg ggaagaatta atgggactac aaagtatact caaaatgcca
      781 acacgccaaa ttattttaaa ctttggacat tatcgtttta ccgatttaaa agaaagtggt
      841 tgcacggtct ttcaaggcaa agacttcatg gttagaaatt atgattacca ccctgcaact
      901 tatgatggtc gctacttact ttatcaacct acagatagcg gtttagctca aattggtcct
      961 gtatcaaggg taactggaag aatggatggt atgaatgagt caggattaac tatgggctat
     1021 aactttatgc accggaagaa accagcaaat ggttttgtat gttatatgat tggtcgtcta
     1081 attcttgaaa attgccgaaa tgtaacagaa gcaatccaat tattgaaaga aataccacac
     1141 agaagctcat ttagctacat actgatggac aaatcattga accatgcaat tgttgaagtg
     1201 acaccgagat caatagatgt tcgttatgat aatatatgta caaatcattt tgaaatatta
     1261 acacatgaaa atcgtaatta tacaaaagaa tctaaagaaa gactggcgcg taccatttca
     1321 caaactaatg ataatttaga tatgactaca gcatttaagt tattcaataa tcctcagtat
     1381 gaaatttaca gtaaactatt caaaagctgg tctggcacca tccatacatc aatgtatcaa
     1441 cctgaaacat taactgcata ttttacattg ggagaaaatg cgccaccaga aataatagat
     1501 ttcaaatcat ggttagatgg tcaagacctt aatatcactc actttactgg caatatagat
     1561 accgacttaa cttttgccaa caaataaata acaccgatat tagagaaatt atgcttccta
     1621 atatcggtgc ttacatcctt tatttattca actttgatat taccatcaat cgtataaaat
     1681 tctaaaacat tgtcgctttt cccaacttta ccattagtaa atgttttatt tttaactacg
     1741 ctatcacccg ttcccggatt tagctttagt atagtgtctt caggtttact atcatactta
     1801 aaacgaatat ctccttgttt agtagaagct tttgcatcta cttttgatgg catgttttta
     1861 aattcaatgt caccatgatc gtttaaaaat atggattgtt taatttgact cttgtcgatt
     1921 tgtagcgtac caataacaac tttaatattg ctatttttaa tgttactttt actaatataa
     1981 gtagaactat tatttgtctt tgaatctagt gcgtcaagat ttgagttttt aataactact
     2041 tcaccgttaa tgctttgaat acttgtgttc tctaaatcaa catcactgat tctaacactt
     2101 cctgctccag atgatagatt taaacgttta atcattttat caggcatttc aatcgttaac
     2161 gttttcttat tctcatgaaa aggattcata tcaattgcat atcctcttgt cttagacctt
     2221 ttatcactaa tcttcaacgt cttatctact atattaatta atatatcatt gtcaccatca
     2281 tatttaactc taaattgatt cccccgtttt atacgaagtt ctgtactatc caaatttata
     2341 gatacattgt caaaagcatc gtctttgaat gttttatcat attgtttagt accatatttg
     2401 tttttatcat agctgaacca aataatggtt gctgctgtaa aaaagacaac aaacactaaa
     2461 agcccaataa aaaagaattt cttcattttc taacgcttcc tttgacagtt ttaatatacc
     2521 aaagtaaata ttttaagatt aaacgataaa tgtattctaa aatcttaaat atgacaatga
     2581 taaatactaa tcctaatcct gaatatgata tggcaaaaaa tacattacta attgagtcca
     2641 caattcctct ggatattgct gatgctaata aaataattgg acaaaacagc agcaaaagtg
     2701 atattaaaaa taatgctaat acaaataatc caactataga tactggtata aggatgacaa
     2761 aaatagatag aatgcctaaa cttaatgaag cagcaacagc tcttgaaata ttttcaaatg
     2821 ttggtttaga ttcagcataa tcaatgacac ttctagcttt tgtttcagct gcaacatctt
     2881 gtgggtcttt taacttcttt aaaatttccc cttcatcaac gccattaagt tcttgctcat
     2941 aaaaataacg ttcatattca tacatttttt gatctcttaa atgcctaggt agcgcatcaa
     3001 gtgctgtttc taattcattt aaataagtaa tcttgttcat ataggtcact catcccaata
     3061 aaattattca ttcgtttttt ataactttaa gacctaaagc tatttactaa aaaattcaat
     3121 acgtttaatt tctaaatctt gataaagtaa gtttaatttt aaatggttat ctctatcaat
     3181 atactttacc gatgagaaat cattgccaac ttttgttctt ttatatccga atccaagatt
     3241 ttcaatcgcg ttatttatcg gcgaaccaat tcccccatca aaattagtat gtatcttttc
     3301 atctcttact aatattatcc ccttaacgat gttatccttt tctctcactt taacggtaag
     3361 atcagggtga gctttactgt tataaaattt aaaacgatca aacttaattt ttttatttac
     3421 ttcatagcct tttttatcaa acttctcatt taatttaata tcgtttattt caagattaga
     3481 caaatccaca gttttcgtat gacgatattg atataaacca attaatacac caaaagaaca
     3541 gattaaaaat aatataaaca caacccagcg attttgaata cgcactgatg tcaccctttc
     3601 tttgtactta tttaaatata gcataaggtg cttcccgttt catccttaac aaaaattaac
     3661 aaagaatttg caattaattt atttcattac cttttattac tataaattgg tataatgttg
     3721 taaatgctaa aggaggtttt tacaatgaca aatttagaaa cttatttcaa aaatagccaa
     3781 cccttaaatg aatacattga tggaatgaag acaaatcaag ataccgttct cagtatctat
     3841 caatcatttg agttaccaga agatgattca cgcatcaaaa aaataaaaga gatgaattat
     3901 tctaaagtat tagtaattac tgaagactgg tgtggagatg caatgatgaa tctcccaatt
     3961 ctaaaacaca tcagtgaagc attaaattta gaagtacgcg tgttccatag agatgatgat
     4021 acaaaactga tcgatcaata tttaacaaat ggtaaatcac gtgcaattcc tatcttcgta
     4081 ttcttgaatg atcaattcga acaagaaaca gtttggggac caagagcaaa tgaagtccaa
     4141 aaatttgtaa ctgatgttcg tgctgataaa ttaccgagca aagatcatcc agactatgca
     4201 gatttagaaa aagaaacaca cttagttata tcaaaccgct ataaaactga cgcttcattt
     4261 tggaaagcag tttataattc catcctaaat aaattagaaa ataaataaat ttacaataag
     4321 acaaactttg aggttgggaa aatttaaaat tcccaacctc aaaattattt ttattaaatg
     4381 tcttttttag cgacatataa gatattcaaa atgaagaata tcactataga acctaatgaa
     4441 attacccatt gccaattgct aaaatcaaag ttagagctaa tcattttatc agttaagtaa
     4501 gcaaaaggga tataatcaaa agcatctaaa actttttcac cgtatcttgg gataaatgta
     4561 ataaaaggtt taataaacgg tacaatgagt actaaaaata cacctaatga aaatatcatt
     4621 gctgtttttt gtgatgcaag tgtgattaag aataaaaaca agccaaagaa tagtaaaaag
     4681 attaggaaga accataaatt atctacatat ttagaaaacg ccaaattttt tccattacta
     4741 gatgcctgca caacaatcaa aatcacat
//
LOCUS       DAKTIO010000184         4734 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.188, whole genome shotgun sequence.
ACCESSION   DAKTIO010000184 DAKTIO010000000
VERSION     DAKTIO010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.188"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            423..3245
                     /gene="fnbB"
                     /locus_tag="O0293_002414"
     CDS             423..3245
                     /gene="fnbB"
                     /locus_tag="O0293_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbB"
                     /protein_id="HCY0281276.1"
                     /translation="MKSNLRYGIRKHKLGAASVFLGTMIVVGMGQEKEAAASEQNNTT
                     VEESGSSATESKASETQTTTNNVNTIDETQSYSATSTEQPSQSTQVTTEEAPKTVQAP
                     KVETSRVDLPSEKVADKETTGTQVDIAQPSNVSEIKPRMKRSTDVTAVAEKEVVEETK
                     ATGTDVTNKVEVEEGSEIVGHKQDTNVVNPHNAERVTLKYKWKFGEGIKAGDYFDFTL
                     SDNVETHGISTLRKVPEIKSTDGQVMATGEIIGERKVRYTFKEYVQEKKDLTAELSLN
                     LFIDPTTVTQKGNQNVEVKLGETTVSKIFNIQYLGGVRDNWGVTANGRIDTLNKVDGK
                     FSHFAYMKPNNQSLSSVTVTGQVTKGNKPGVNNPTVKVYKHIGSDDLAESVYAKLDDV
                     SKFEDVTDNMSLDFDTNGGYSLNFNNLDQSKNYVIKYEGYYDSNASNLEFQTHLFGYY
                     NYYYTSNLTWKNGVAFYSNNAQGDGKDKLKEPIIEHSTPIELEFKSEPPVEKHELTGT
                     IEESNDSKPIDFEYHTAVEGAEGHAEGTIETEEDSIHVDFEESTHENSKHHADVVEYE
                     EDTNPGGGQVTTESNLVEFDEDSTKGIVTGAVSDHTTIEDTKEYTTESNLIELVDELP
                     EEHGQAQGPIEEITENNHHISHSGLGTENGHGNYGVIEEIEENSHVDIKSELGYEGGQ
                     NSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNNGNQSFEEDTEKDKPKYEQ
                     GGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPNYQFGGHNSVDFEEDTLPQVSGHN
                     EGQQTIEEDTTPPIVPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEVPTEPGK
                     PIPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVVPTKKAQSKKSELPETGGEESTN
                     NGMLFGGLFSILGLALLRRNKKNHKA"
     gene            3425..4291
                     /gene="galU"
                     /locus_tag="O0293_002415"
     CDS             3425..4291
                     /gene="galU"
                     /locus_tag="O0293_002415"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCY0281277.1"
                     /translation="MKKIKKAIIPAAGLGTRFLPATKAMPKEMLPILDKPTIQYIVEE
                     AARAGIEDIIIVTGRHKRAIEDHFDSQKELEMVLKEKGKSELLEKVQYSTELANIFYV
                     RQKEQKGLGHAISSARQFIGNEPFAVLLGDDIVESEVPAVKQLIDVYEETGHSVIGVQ
                     EVPEADTHRYGIIDPLTKNGRQYEVKKFVEKPAQGTAPSNLAIMGRYVLTPEIFDYLK
                     TQKEGAGNEIQLTDAIERMNNDNQVYAYDFEGERYDVGEKLGFVKTTIEYALKDDSMR
                     EELTRFIKALGL"
     gene            complement(4502..>4734)
                     /gene="sarU"
                     /locus_tag="O0293_002416"
     CDS             complement(4502..>4734)
                     /gene="sarU"
                     /locus_tag="O0293_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarU"
                     /protein_id="HCY0281278.1"
                     /translation="KNEILNLKDLFESIRFMYPQIVRSVNRLNNKGMLIKERSHADER
                     IVLIKINKIQYNTIKSIFTDTSKILKPRKFFF"
BASE COUNT         1847 a          689 c          910 g         1288 t
ORIGIN      
        1 cttgaaatac ggctgttttt gtataaataa tcattaaaat caaacaaaaa ccgaacgata
       61 taaaaattat agtgacgtta ttaatacaat gaaaggcgtt tcagtgactt gttaatgatg
      121 cattgtacag gcgattatta tgtatggttt agtatattgt tttaaattag atgaatttga
      181 atttaattta aaaagtaagt ataaaaaata caagcctgtt atatcaaggt gttcaagagc
      241 tttgtatgca atatatatgt gagtttcaaa taataaaaaa ctcattatac ataaagttaa
      301 caacaatctt aactttttat taactcgctt tttttcattg cttttaaaaa ccgaacaata
      361 tagaattgca tttattgagt tttaaaaata aatgaatttt gcatttaagg gagaatatta
      421 tagtgaaaag caatcttaga tacggcataa gaaaacacaa attgggagcg gcctcagtat
      481 tcttaggaac aatgatcgtt gttggaatgg gacaagaaaa agaagctgca gcatcggaac
      541 aaaacaatac tacagtagag gaaagtggga gttcagctac tgaaagtaaa gcaagcgaaa
      601 cacaaacaac tacaaataac gttaatacaa tagatgaaac acaatcatac agcgcgacat
      661 caactgagca accatcacaa tcaacacaag taacaacaga agaagcaccg aaaactgtgc
      721 aagcaccaaa agtagaaact tcgcgagttg atttgccatc ggaaaaagtt gctgataagg
      781 aaactacagg aactcaagtt gacatagctc aaccaagtaa cgtctcagaa attaaaccaa
      841 gaatgaaaag atcaactgac gttacagcag ttgcagagaa agaagtagtg gaagaaacta
      901 aagcgacagg tacagatgta acaaataaag tggaagtaga agaaggtagt gaaattgtag
      961 gacataaaca agatacgaat gttgtaaatc ctcataacgc agaaagagta accttgaaat
     1021 ataaatggaa atttggagaa ggaattaagg cgggagatta ttttgatttc acattaagcg
     1081 ataatgttga aactcatggt atctcaacac tgcgtaaagt tccggagata aaaagtacag
     1141 atggtcaagt tatggcgaca ggagaaataa ttggagaaag aaaagttaga tatacgttta
     1201 aagaatatgt acaagaaaag aaagatttaa ctgctgaatt atctttaaat ctatttattg
     1261 atcctacaac agtgacgcaa aaaggtaacc aaaatgttga agttaaattg ggtgagacta
     1321 cggttagcaa aatatttaat attcaatatt taggtggagt tagagataat tggggagtaa
     1381 cagctaatgg tcgaattgat actttaaata aagtagatgg gaaatttagt cattttgcgt
     1441 acatgaaacc taacaaccag tcgttaagct ctgtgacagt aactggtcaa gtaactaaag
     1501 gaaataaacc aggggttaat aatccaacag ttaaggtata taaacacatt ggttcagacg
     1561 atttagctga aagcgtatat gcaaagcttg atgatgtcag caaatttgaa gatgtgactg
     1621 ataatatgag tttagatttt gatactaatg gtggttattc tttaaacttt aataatttag
     1681 accaaagtaa aaattatgta ataaaatatg aagggtatta tgattcaaat gctagcaact
     1741 tagaatttca aacacacctt tttggatatt ataactatta ttatacaagt aatttaactt
     1801 ggaaaaatgg cgttgcattt tactctaata acgctcaagg cgacggcaaa gataaactaa
     1861 aggaacctat tatagaacat agtactccta tcgaacttga atttaaatca gagccgccag
     1921 tggagaagca tgaattgact ggtacaatcg aagaaagtaa tgattctaag ccaattgatt
     1981 ttgaatatca tacagctgtt gaaggtgcag aaggtcatgc agaaggtacc attgaaactg
     2041 aagaagattc tattcatgta gactttgaag aatcgacaca tgaaaattca aaacatcatg
     2101 ctgatgttgt tgaatatgaa gaagatacaa acccaggtgg tggtcaggtt actactgagt
     2161 ctaacctagt tgaatttgac gaagattcta caaaaggtat tgtaactggt gctgttagcg
     2221 atcatacaac aattgaagat acgaaagaat atacgactga aagtaatctg attgaactag
     2281 tagatgaact acctgaagaa catggtcaag cgcaaggacc aatcgaggaa attactgaaa
     2341 acaatcatca tatttctcat tctggtttag gaactgaaaa tggtcacggt aattatggcg
     2401 tgattgaaga aatcgaagaa aatagccacg tggatattaa gagtgaatta ggttacgaag
     2461 gtggccaaaa tagcggtaat cagtcatttg aggaagacac agaagaagat aaaccgaaat
     2521 atgaacaagg tggcaatatc gtagatatcg atttcgatag tgtacctcaa attcatggtc
     2581 aaaataatgg taaccaatca ttcgaagaag atacagagaa agacaaacct aagtatgaac
     2641 aaggtggtaa tatcattgat atcgacttcg acagtgtgcc acatattcac ggattcaata
     2701 agcacactga aattattgaa gaagatacaa ataaagataa accaaattat caattcggtg
     2761 gacacaatag tgttgacttt gaagaagata cacttccaca agtaagtggt cataatgaag
     2821 gtcaacaaac gattgaagaa gatacaacac ctccaatcgt gccaccaacg ccaccgacac
     2881 cagaagtacc aagcgagccg gaaacaccaa caccaccgac accagaagta ccaagcgagc
     2941 cggaaacacc aacaccgcca acgccagagg taccaactga acctggtaaa ccaataccac
     3001 ctgctaaaga agaacctaaa aaaccttcta aaccagtgga acaaggtaaa gtagtaacac
     3061 ctgttattga aatcaatgaa aaggttaaag cagtggtacc aactaaaaaa gcacaatcta
     3121 agaaatctga actacctgaa acaggtggag aagaatcaac aaacaacggc atgttgttcg
     3181 gcggattatt tagcatttta ggtttagcgt tattacgcag aaataaaaag aatcacaaag
     3241 cataatcaat ccaaaattga caggtttatt tcataaatta tatgaagtaa gcctgttttt
     3301 taaaattaaa acaaatttcc caagaaataa ttacatactc aatgacacta tgaaggcgtt
     3361 ctaattagtg ttaaaatgac gttgatacat agatttaata cttaggaaaa ggagcacatt
     3421 aactttgaaa aaaataaaaa aggcaatcat tcccgctgct ggtttaggga ctagattttt
     3481 accagcaact aaagcgatgc caaaggaaat gcttcctatc ttagataaac ccacaataca
     3541 atatatcgtt gaagaagctg caagagctgg aattgaagat attattatag tgacaggtcg
     3601 ccacaaacgc gcgattgaag atcattttga tagtcaaaaa gaattagaaa tggtgttaaa
     3661 agaaaaaggt aaatctgaat tactagagaa agttcagtat tcaacggaac ttgcgaatat
     3721 tttttatgta aggcagaaag aacaaaaagg tttagggcat gcgattagtt ctgcgcgtca
     3781 atttatcggc aatgaaccat ttgcggtatt attaggtgac gatattgttg aatctgaagt
     3841 gccagcagtg aaacagttga ttgacgttta tgaagaaaca gggcactcag ttataggtgt
     3901 ccaagaagtt ccggaagcag atactcatcg ttatggaatt attgacccat taactaaaaa
     3961 cggacgtcag tatgaagtga aaaagtttgt tgaaaagcca gcacaaggta cggcaccatc
     4021 aaacctagct attatgggac gttatgtatt aacgcctgaa attttcgatt atttaaaaac
     4081 gcaaaaagaa ggcgcaggta acgaaattca gttaacagat gcgattgaac gtatgaataa
     4141 tgataaccaa gtgtatgcat atgacttcga aggcgaacgt tatgacgttg gagaaaaatt
     4201 aggcttcgtt aaaactacca tagaatatgc attgaaagat gacagtatgc gagaagaatt
     4261 aacacgattt attaaagcat taggtttata acattaaaag catgatactc agtaaaattg
     4321 gaacacgaaa tggtgaacta aattgaatga gtgtcatgct taatttatgt gttgtgatat
     4381 atagtagggt attgaaataa tataaaaaca aaagaaagtt aattatgtat ttttatttat
     4441 actaaattga gaagtagtat gattgcttat ttattttatc aagaggtaaa tctgtttaaa
     4501 tttaaaagaa aaattttctt ggtttgagaa tcttggaagt atctgtgaat atgcttttaa
     4561 tagtgttata ttgtatttta tttattttga ttaacacaat cctttcatct gcatgggatc
     4621 gttctttgat tagcatacct ttattattta atctattaac tgacctaaca atttgaggat
     4681 acataaatct aatactttca aacaaatctt ttaaatttag tatctcattt tttc
//
LOCUS       DAKTIO010000185         4638 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.189, whole genome shotgun sequence.
ACCESSION   DAKTIO010000185 DAKTIO010000000
VERSION     DAKTIO010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.189"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(124..1056)
                     /gene="cysK"
                     /locus_tag="O0293_002417"
     CDS             complement(124..1056)
                     /gene="cysK"
                     /locus_tag="O0293_002417"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCY0281279.1"
                     /translation="MAQKPVDNITQIIGGTPVVKLRNVVDDNAADVYVKLEYQNPGGS
                     VKDRIALAMIEKAEREGKIKPGDTIVEPTSGNTGIGLAFVCAAKGYKAVFTMPETMSQ
                     ERRNLLKAYGAELVLTPGSEAMKGAIKKAKELKEEHGYFEPQQFENPANPEVHELTTG
                     PELLQQFEGKTIDAFLAGVGTGGTLSGVGKVLKKEYPNIEIVAIEPEASPVLSGGEPG
                     PHKLQGLGAGFIPGTLNTEIYDSIIKVGNDTAMEMSRRVAKEEGILAGISSGAAIYAA
                     IQKAKELGKGKTVVTVLPSNGERYLSTPLYSFDD"
     gene            complement(1235..2116)
                     /gene="hslO"
                     /locus_tag="O0293_002418"
     CDS             complement(1235..2116)
                     /gene="hslO"
                     /locus_tag="O0293_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCY0281280.1"
                     /translation="MTHDYIVKALAFDGEIRAYAALTTETVQEAQTRHYTWPTASAAM
                     GRTMTATAMMGAMLKGDQKLTVTVDGQGPIGRIIADANAKGEVRAYVDHPQTHFPLNE
                     QGKLDVRRAVGTNGSIMVVKDVGMKDYFSGASPIVSGELGEDFTYYYATSEQTPSSVG
                     LGVLVNPDNTIKAAGGFIIQVMPGAKDETISKLEKAISEMTPVSKLIEQGLTPEGLLN
                     EILGEDHVQILEKMPVQFECNCSHEKFLNAIKGLGEAEIQNMIKEDHGAEAVCHFCGN
                     KYKYTEEELNVLLESLA"
     gene            complement(2345..4438)
                     /gene="ftsH"
                     /locus_tag="O0293_002419"
     CDS             complement(2345..4438)
                     /gene="ftsH"
                     /locus_tag="O0293_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCY0281281.1"
                     /translation="MQKAFRNVLVIVIIGVIIFGLFSYLNGNGNMPKQLTYNQFTEKL
                     EKGDLKTLEIQPQQNVYMVSGKTKNDEDYSSTILYNNEKELQKITDAAKKQNGVKLTI
                     KEEEKQSVFVSILSTLIPVVVIALLFIFFLSQAQGGGSGGRMMNFGKSKAKMYDNNKR
                     RVRFSDVAGADEEKQELIEIVDFLKDNKKFKEMGSRIPKGVLLVGPPGTGKTLLARAV
                     AGEAGAPFFSISGSDFVEMFVGVGASRVRDLFDNAKKNAPCIIFIDEIDAVGRQRGAG
                     VGGGHDEREQTLNQLLVEMDGFGENEGIIMIAATNRPDILDPALLRPGRFDRQIQVGR
                     PDVKGREAILHVHAKNKPLDETVDLKAISQRTPGFSGADLENLLNEASLIAVREGKKK
                     IDMRDIEEATDRVIAGPAKKSRVISKKERNIVAHHEAGHTIIGMVLDEAEVVHKVTIV
                     PRGQAGGYAMMLPKQDRFLMTEQELLDKICGLLGGRVSEDINFNEVSTGASNDFERAT
                     QIARSMVTQYGMSKKLGPLQFGHSNGQVFLGKDMQGEPNYSSQIAYEIDKEVQRIVKE
                     QYERCKQILLEHKEQLILIAETLLTEETLVAEQIQSLFYEGKLPEIDYDAAKVVKDED
                     SEFNDGKFGKSYEEIRKEQLEDGQRDESEDRKEEKDIAEDKKEADKSDEKDEPAHQQA
                     PNIEKPYDPNHPDNK"
BASE COUNT         1372 a          954 c          646 g         1666 t
ORIGIN      
        1 attcactgga tatttttatc agaaaaatgt ttaatttaac gctcatattt ttaaaaatat
       61 acatggtaga aaaaaagagc aagttatctc aaaaagataa ctcactcttt taaatgacat
      121 taattagtca tcgaatgaat ataaaggtgt tgataagtag cgttcaccat tactcggcaa
      181 tactgttact actgttttac cttttcctaa ttcttttgct ttttgaatgg cagcataaat
      241 cgcagcacct gatgaaatac ctgctaaaat accttcctct ttagcaactc gacgagacat
      301 ttccatcgct gtatcatttc ctactttaat aatactgtca tagatttctg tattcaaagt
      361 gcctggaata aatccagcac ctaaaccttg taatttatgt ggacctggct caccaccgct
      421 caatactgga gaagcctcag gctctatagc aacaatttcg atgttaggat attctttttt
      481 cagaacttta cctacaccag ataacgtacc accagtacca acaccagcta ggaacgcatc
      541 gatagttttc ccttcaaatt gttgtaataa ctcaggacct gtagttaact catgaacttc
      601 agggttcgca gggttttcaa attgttgtgg ctcgaagtaa ccatgttctt ctttcaattc
      661 tttagctttt ttaattgcac ctttcatcgc ttctgatcca ggcgttaaaa ctaattccgc
      721 accgtatgct tttaataaat tacgacgctc ttggctcatt gtttcgggca tagtaaatac
      781 tgctttatat cctttagcag cacatacaaa tgctaaaccg atacctgtat taccacttgt
      841 tggttctaca attgtatcgc caggtttaat tttgccttct cgctctgctt tttcaatcat
      901 tgctaaagca attctatcct ttacagaacc acctggattt tgatattcca attttacata
      961 aacatctgct gcattgtcat ctactacatt tctcaatttg actaccggtg taccgccaat
     1021 aatttgagta atattatcta ctggtttttg tgccattaag aacactcctt tttagttagt
     1081 tgttgaaatc ttagtttatt tatcggatat agaattattt tatcaaataa attctagaaa
     1141 gcaaacaaaa tcactaagga ttatacaact atttagttct acatttcact aaaccgctaa
     1201 aaataaacat tttagcgtat tgatttaaat taaattacgc taaactttct agcaacacgt
     1261 ttaattcttc ttcagtatat ttatatttat ttccacagaa atgacatact gcttcagcac
     1321 catgatcttc tttaatcata ttttgaatct cagcctcgcc caatccttta atagcattta
     1381 aaaatttctc atgactacaa ttacattcaa attgaacagg cattttctct aaaatttgca
     1441 catggtcttc acctaagatt tcgtttagta atccttctgg cgttaatcct tgttcaatta
     1501 atttagaaac tggtgtcatt tcactaattg ctttttctaa ttttgaaatt gtttcatctt
     1561 tggcacctgg cataacttga atgataaatc ctcctgctgc tttaatcgta ttatcaggat
     1621 ttaccaatac accaagacct accgatgaag gtgtttgttc acttgtagca taataataag
     1681 taaaatcttc accaagttct cctgaaacaa ttggacttgc tccagagaaa tagtctttca
     1741 ttccaacgtc tttaacaacc ataatagatc catttgtccc taccgctcgt cttacatcaa
     1801 gtttaccttg ctcatttaat ggaaaatgag tttgtggatg gtctacataa gcacgcacct
     1861 cgcctttagc atttgcatcg gcaataattc gtccaatagg tccttggcca tctacagtga
     1921 cagttaattt ttgatcacct ttcaacattg cgcccatcat agctgttgct gtcattgttc
     1981 ttcccattgc agcagatgct gtcggccatg tataatgtct cgtttgtgct tcttgaacag
     2041 tttcagttgt caaagcagca taagccctaa tctctccatc aaatgctaat gctttaacaa
     2101 tataatcgtg tgtcattatt tcaatctcct ctattactct atatttaaaa aattacttta
     2161 cttcataaaa tgcaacaatt gtacttattc tacacccatc attctaaata atgaagtaac
     2221 ttgttttaca attattttct gctataacaa ttcaacgact taaaatctaa tacgtatttt
     2281 caaaaacgat aaaagtacct ctttctataa ctttatcata gaaagaggta ctgaatataa
     2341 tcgattattt attgtctggg tgatttggat cgtaaggttt ttcgatattt ggggcttgtt
     2401 gatgtgctgg ttcatctttt tcatcagatt tatcagcttc ttttttatcc tcagcaatat
     2461 ctttttcttc tttacgatct tcactttcgt cacgttgtcc atcttctaat tgctctttac
     2521 gaatctcttc ataagattta ccgaatttac catcattaaa ttcagaatct tcatctttaa
     2581 caactttagc tgcatcataa tcaatttcag gtaatttacc ttcgtagaat aatgattgaa
     2641 tttgttcagc aactaatgtt tcttctgtta ataatgtttc agcaattaaa attaattgtt
     2701 ctttgtgctc taataaaatt tgtttacaac gttcgtattg ttctttaacg attcgttgaa
     2761 cttctttatc aatttcatat gcgatttggc ttgaataatt aggctcacct tgcatatctt
     2821 tacctaagaa tacttgacca ttgctatgac cgaactgtaa tggtcctaat tttttactca
     2881 taccatattg cgtaaccatt gagcgtgcga tttgtgttgc acgttcgaag tcatttgaag
     2941 cacctgttga tacttcgtta aagttaatat cttctgatac acgtccacca agtaaaccac
     3001 agattttatc taataactct tgttcagtca ttaagaaacg atcttgttta ggtagcatca
     3061 ttgcataacc acctgcttgt ccacgtggaa caatagtaac tttatgcact acttctgcct
     3121 catcaagtac cataccgata attgtatgac cagcttcgtg atgagcaaca atattacgtt
     3181 ctttcttaga aataactcga gatttcttag caggtccggc tataactcta tccgttgcct
     3241 cttcgatatc tctcatgtca attttctttt taccttcacg tacagcaatt aaagatgctt
     3301 catttaataa gttctctaaa tcagcacctg agaaaccagg tgtacgttgt gaaattgctt
     3361 ttaaatcaac cgtttcatca agtggtttgt ttttagcatg aacatgaaga attgcttcac
     3421 ggcctttcac atctggacga ccaacttgaa tttgtctatc aaaacgacct ggacgtaata
     3481 aggctgggtc aaggatatca ggacggtttg tagcagctat cataatgata ccttcatttt
     3541 caccgaaacc atccatttca actaataatt ggtttagggt ttgttcacgt tcatcatgac
     3601 cgccaccaac acctgcacca cgttgacgac caacagcatc aatctcatcg ataaagatga
     3661 tacaaggcgc gtttttctta gcattatcga ataagtcacg aacacggctc gcaccaacac
     3721 caacaaacat ctctacaaag tctgaaccac taatagagaa gaatggtgcg ccagcttcac
     3781 ctgcaaccgc tctagcaagt aatgttttac cagtacctgg aggtccaaca agtaagacac
     3841 ctttaggaat cctagatccc atttctttga attttttatt atctttcaag aaatcaacaa
     3901 tttcaattaa ttcttgtttt tcttcatctg cccctgctac atcagagaaa cgaacacgac
     3961 gtttattatt atcgtacatt tttgctttag atttaccaaa gttcatcata cgaccgccac
     4021 taccgccacc ttgtgcttgg cttaggaaga aaataaataa taacgctatg actacaactg
     4081 gaattaatgt tgaaagtata ctcacaaaga cactttgttt ttcttcttct ttaatcgtta
     4141 attttacacc gttttgcttt ttagcagcat cagtaatttt ttgtaattct ttttcgttgt
     4201 tatataaaat agttgatgaa tagtcttcat catttttcgt tttaccactt accatataga
     4261 cattttgttg tggttggatt tctaaagttt taaggtcacc tttttccaac ttctcagtaa
     4321 attgattata tgtaagctgt ttcggcatat ttccattacc gtttaaatat gaaaatagac
     4381 caaaaataat aacgcctatt attacgataa ctagcacatt gcgaaaagct ttctgcatgc
     4441 gtcatttcct cctacttccc ataataaaac taacaaaaat tgtaacatat gacatgtttt
     4501 ataagctagt aattgagctt aacatcaatg tcattcacac ccgtattagt tttatcaatc
     4561 tctatatttt tcaacttttc aagacatatg tcttgtctaa aattaaatat atatgaaata
     4621 catttttcat atttttga
//
LOCUS       DAKTIO010000186         4575 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.191, whole genome shotgun sequence.
ACCESSION   DAKTIO010000186 DAKTIO010000000
VERSION     DAKTIO010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.191"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(98..619)
                     /locus_tag="O0293_002420"
     CDS             complement(98..619)
                     /locus_tag="O0293_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwhD family protein"
                     /protein_id="HCY0281282.1"
                     /translation="MEVISLSEKKGFNFNIIKNDPLDGHKGTNIGSISLDNIAPVFID
                     VANKEAFIDIGGMHARAKVEKGVKWITDKAAVEGDEAKEYWLCWVTTERNEQGPYYAG
                     LTACYLLVNKAIRRGYKSMPEHVNMMDKSMKHHIIIDQIGDENKAILKDFLMNHDEGM
                     WKHSSDALHQAFN"
     gene            complement(647..1942)
                     /locus_tag="O0293_002421"
     CDS             complement(647..1942)
                     /locus_tag="O0293_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCY0281283.1"
                     /translation="MTNAYVDLKLVEEKVFKDPIHRYIHVEDQLIWDLIKTKEFQRLR
                     RIRQLGTLYLSFHTAEHSRFGHSLGVYEIVRRLIDESFIGHDAWDNKDRPLALCAALL
                     HDLGHGPFSHSFEKIFNTDHEAYTQAIITGDTEVNAVLRKVAPEFPREVAEVINKTHH
                     NKLVISMISSQIDADRMDYLQRDAYFTGVSYGAFDMERILRLMRPSKDEVLIKESGMH
                     AVENFIMSRYQMYWQIYFHPVSRGGEVLLNNCLKRAKQLYNEGYEFKLHPHDFIPFFE
                     ETVTIEQYVELDEAVVTYYLEKWTKEDDAILSDLASRFINRDLFKYIPFDGSIITISE
                     LQELFEAGGINPDYYFVSEAFSDLPYDYDRPGSNRKPIHLLRQDGTIREISNQSLVIH
                     SITGINRQDYKLYYPREMVAKIKDKTIREAIENLINELN"
     gene            complement(1982..2458)
                     /locus_tag="O0293_002422"
     CDS             complement(1982..2458)
                     /locus_tag="O0293_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0281284.1"
                     /translation="MFKVRQATEKDVVQIRDVATKAWFNTYLNIYAATTVNHLLEASY
                     NEHHLKKRLQEQLFLVVEEGNDIVGFANFIYGEELYLSAHYVKPESQHTGYGTALLNE
                     GLSRFEDKFEGVYLEVDNKNEEAVAYYKEQGFTILRSYEPEMYGEKLDLALMYKAF"
     gene            complement(2627..3163)
                     /locus_tag="O0293_002423"
     CDS             complement(2627..3163)
                     /locus_tag="O0293_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HCY0281285.1"
                     /translation="MITVLFGGSRPNGNTAQLTKFALQDLEYQWIDVTQHQFKPIRDV
                     RHTAETITSYDDDYLPILDKILASDTIIFASPVYWYSISAPLKAFIEHWSETLQDKRY
                     PNFKAQMAEKDFRVILVGGDCPKIKAKPAITQMKYSLDFLGATLNGYIIGTAEKPGDI
                     MKDNYALARATEWNSILQ"
     gene            complement(3374..4312)
                     /locus_tag="O0293_002424"
     CDS             complement(3374..4312)
                     /locus_tag="O0293_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCY0281286.1"
                     /translation="MKQFVNLGKSDVEVFPIALGTNAVGGHNLYPNLDEEQGKDVVRQ
                     AINHGINLLDTAYIYGPERSEELVGEVVKEYPREQIKIATKGSHEFDENQEVHQNNQP
                     EYLKQQVENSLKRLQTDYIDLYYIHFPDNNTPKDQAVAALQELKEQGKIKAIGVSNFT
                     LDQLKEANKDGYVDVVQLEYNLLHRENEAVLQYCVDHQITFIPYFPLASGILAGKYDE
                     NTKFSDHRTTRRDFKPGVFEENVRRVKALESIAAAHQTSIANIVLAFYLTRPAIDVII
                     PGAKRAEQVVENIKAADIVLSDDEIQYIDELFPIED"
BASE COUNT         1442 a          873 c          619 g         1641 t
ORIGIN      
        1 tcaatctaag acattatttt cacctcacga tttacgtcgt cattgagaaa tgaatcatat
       61 ctttatagat taattgggaa attttagttt ctaatattta attaaatgct tgatgtaaag
      121 catcagaaga atgcttccac ataccttcat catggttcat taaaaagtct tttaaaatag
      181 ctttattctc gtcaccaatt tgatctatga taatatgatg tttcattgat ttatccatca
      241 tattaacatg ttcaggcata cttttataac cacgacgaat tgctttattc actaataaat
      301 agcacgctgt taaaccagcg taatatggtc cttgttcatt acgttctgtt gttacccaac
      361 acaaccaata ttctttagct tcatcgcctt caacagcagc tttatcagta atccatttca
      421 cacctttttc aactttggca cgagcatgca tgcctccaat atcaataaat gcttctttgt
      481 tagcaacatc gataaaaact ggtgcaatat tgtctaagct aattgaacca atatttgtac
      541 ctttatgacc atctagaggg tcatttttta tgatattaaa attaaagcct tttttctcag
      601 acaatgatat aacctccata tttaacgata acaattttag ccctgtttaa ttaagctcat
      661 taatcaaatt ttcaatagct tctctaattg tcttatcttt aatctttgca accatttctc
      721 taggataata taatttatag tcttggcgat taatgcctgt aatactatga atgactaatg
      781 attgattgct tatttctcta atcgtaccat cttgtcttaa taaatgaatc ggtttgcgat
      841 ttgaccccgg tcgatcatag tcatatggca aatcagaaaa tgcttcactc acaaaataat
      901 aatctggatt aataccacct gcttcaaaca gttcttgcag ttctgatatt gtaataattg
      961 agccatcaaa tggaatatat ttaaataagt ctcgattaat aaatcgactt gctaaatcac
     1021 ttaaaatagc atcatcttct tttgtccatt tttccaaata atacgtaact accgcttcat
     1081 cgagttcaac atattgttca atcgtaactg tctcttcaaa aaatggaata aaatcatgtg
     1141 gatgcaactt aaattcatag ccttcattat aaagctgttt tgcgcgtttc aaacaattat
     1201 taagcagcac ttctccacca cgactaactg ggtggaagta aatttgccaa tacatttgat
     1261 aacgactcat aataaagttt tcaactgcat gcataccact ttctttgatt agtacttcat
     1321 ctttagaagg tcgcattaat cttaaaatac gctccatatc aaaagcacca tatgatacac
     1381 ctgtgaaata cgcatcacgt tgtaaataat ccattctatc cgcatcgatt tgtgacgaaa
     1441 tcatcgaaat gaccaattta ttatgatgcg ttttattaat tacttccgca acttctcttg
     1501 gaaactcagg cgccacttta cgtaatacag cattcacctc agtatctcca gtaataatcg
     1561 cttgtgtgta tgcttcatgg tctgtattaa atattttttc aaaactatgt gaaaatggac
     1621 catgtcctaa atcatgtaat aatgcagcac ataatgccaa cggtctatct ttattgtccc
     1681 atgcatcatg accaataaat gactcatcaa ttaatcgtct aactatttca tacacaccta
     1741 aagaatgtcc aaagcgacta tgttctgctg tgtgaaaaga taggtacagt gttcctagtt
     1801 gtctaattcg acgtaacctt tggaattcct tagttttaat taaatcccat atcaattgat
     1861 cttcaacatg aatatatcga tgtatcgggt ctttaaaaac tttttcttct actaatttta
     1921 aatctacata tgcgttagtc attattcccc tccttttcgt ttaatataat atttaattta
     1981 cttaaaatgc tttgtacata agtgctaagt ctaacttttc gccatacatt tctggctcat
     2041 aagagcgtaa gattgtaaaa ccttgctctt tatagtaagc tactgcttct tcatttttat
     2101 tatctacttc taagtaaaca ccttcaaatt tatcttcaaa acgtgataat ccttcattta
     2161 acaatgctgt accataacct gtatgttgcg attctggttt aacataatga gctgataaat
     2221 ataattcttc accgtaaata aagttagcaa agccaacgat gtcattacct tcttcaacga
     2281 ctaagaataa ttgttcttga agtcttttct ttaaatgatg ttcattatat gaagcttcta
     2341 acaagtgatt aactgttgtc gcagcgtata tatttaagta tgtattaaac caagctttag
     2401 ttgcgacatc tctaatttga acaacatctt tttcagttgc ttgtcttacc ttgaacatga
     2461 ctttctcccc ttattaacaa gttttaataa cggcattata ccacaacttg ctcaatactt
     2521 aataaacaat gattgtctat tcaatttata tatttatatt ttccgttaaa attaaaaata
     2581 aaaaataacg aagcaaaaaa tcacttcgtt tagtatgagg tatgtcttat tgcaatatac
     2641 tattccactc agttgcacgt gctaaggcat agttgtcttt catgatgtca ccaggctttt
     2701 cagcagttcc aataatataa ccatttaaag tggcacctaa aaagtctaaa ctatatttca
     2761 tttgcgtaat tgctggcttc gcttttattt ttggacaatc tccaccaact aaaataactc
     2821 taaaatcctt ttcggccatt tgtgccttaa aattaggata tcgtttatct tgtaatgttt
     2881 ctgaccaatg ttcgataaat gctttcaatg gtgctgaaat gctataccaa tacactggtg
     2941 atgcaaaaat aattgtatca ctagccaata ttttatctag aatcggcaaa tagtcatcgt
     3001 catatgaagt aatagtctct gctgtatgtc tcacgtcacg tatcggttta aactgatgtt
     3061 gtgtcacgtc aatccattga tactctaaat cttgcaaagc gaattttgtt aattgtgcag
     3121 tattaccgtt tggtctactc ccaccaaaca aaacagtaat cattttagcc taacctcact
     3181 tttgattaat aaatatctgt gtttttcgtt acctaattat actatcataa gctttgccta
     3241 ccgaatagta aaacgcttac aacttttata taaatttgac gaaatttcgt catgccttat
     3301 ataacgtcgt ttgtgatacg gggctaattc atgatgaaat tagatacata tatcaccatt
     3361 aaatacaatt catttagtct tcaatcggaa acagttcatc gatatattga atctcatcat
     3421 ctgataaaac gatatctgca gctttaatat tttcaacgac ttgttctgca cgttttgcac
     3481 caggaataat cacatcgata gctggtctcg ttaaataaaa tgctaataca atgttcgcaa
     3541 ttgaagtttg atgtgctgca gctatgcttt ccaaagcttt tacgcgacgc acattttctt
     3601 caaatacacc tggtttaaaa tcacgacgtg tagtacgatg gtcactaaat ttagtgttct
     3661 catcatattt tccagctaaa ataccggatg ctaatgggaa atatggaata aatgtgattt
     3721 ggtgatcaac acaatattgt aatactgcct cattttcgcg atgcaataaa ttatattcta
     3781 actgtacaac atcaacgtaa ccatctttat ttgcttcttt aagttgatct aatgtgaaat
     3841 ttgatacacc aattgcttta atcttccctt gttccttaag ctcttgtaat gctgcaactg
     3901 cttgatcttt cggagtgttg ttatccggaa aatgaatata atataaatcg atataatcag
     3961 tttgtagacg tttcaaacta ttctcaactt gttgttttaa atattccggt tgattgttct
     4021 gatgtacttc ttgattttca tcaaattcat gagacccttt cgtagcaatt ttaatttgct
     4081 ctcgcggata ttctttaaca acttctccaa ccaattcttc tgatcgttct ggcccataaa
     4141 tatatgccgt atctaataaa ttaataccat gattaatggc ttgacgaaca acatcttttc
     4201 cttgttcttc atctaagttc ggatataaat tatgcccacc tactgcgttc gtcccaagtg
     4261 cgattggaaa cacttcaaca tcagatttac ctaagtttac aaattgcttc attagaccca
     4321 gctccttatc tttataactt ttccactatc acactatcat gatttaatac aacttccaaa
     4381 tgttagaatt agagcttgaa tttaagcggt gtaataggaa aacgttatat aaaactcagt
     4441 taaaataagt attgcttaaa ttgatatgca tatagcgcga ttgctcgttg acctttaaat
     4501 tacatgtact tttgaatcag aaattgaaaa acatttaaga acgacaatat taaagccatt
     4561 gcaaatttaa tttgg
//
LOCUS       DAKTIO010000187         4503 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.192, whole genome shotgun sequence.
ACCESSION   DAKTIO010000187 DAKTIO010000000
VERSION     DAKTIO010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.192"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            164..694
                     /locus_tag="O0293_002425"
     CDS             164..694
                     /locus_tag="O0293_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCY0281287.1"
                     /translation="MQIRQIHQHDFAQVDQLIRTAFENSEHGYGNESELVDQIRLSDT
                     YDNTLELVAVLQNEVVGHGLLSEVYLDNEAQREIGLVLAPVSVDIHHQNKGIGKRLIQ
                     ALEREAILKGYNFISVLGWPTYYANLGYQRASMYDIYPPYDGIPDEAFLIKELKVNSL
                     AGKTGTINYTSAFEKI"
     gene            957..3740
                     /gene="clfA"
                     /locus_tag="O0293_002426"
     CDS             957..3740
                     /gene="clfA"
                     /locus_tag="O0293_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfA"
                     /protein_id="HCY0281288.1"
                     /translation="MNMKKKEKHAIRKKSIGVASVLVGTLIGFGLLSSKEADASENSV
                     TQSDSASNESKSNDSSSVSAAPKTDDTNVSDTKTSSNTNNGETSVAQNPAQQETTQSS
                     STNATTEETPVTGEATTTTTNQANTPATTQSSNTNAEELVNQTSNETTSNDTNTVSSV
                     NSPQNSTNAENVSTTQDTSTEATPSNNESAPQSTDASNKDVVNQAVNTSAPRMRAFSL
                     AAVAADAPAAGTDITNQLTNVTVGIDSGTTVYPHQAGYVKLNYGFSVPNSAVKGDTFK
                     ITVPKELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVNTKDDVKATLT
                     MPAYIDPENVKKTGNVTLATGIGSTTANKTVLVDYEKYGKFYNLSIKGTIDQIDKTNN
                     TYRQTIYVNPSGDNVIAPVLTGNLKPNTDSNALIDQQNTSIKVYKVDNAADLSESYFV
                     NPENFEDVTNSVNITFPNPNQYKVEFNTPDDQITTPYIVVVNGHIDPNSKGDLALRST
                     LYGYNSNIIWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPEDSDSDPG
                     SDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSDNDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSASDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSESDSDSESDSDSDSDSDSDSDSDSDSDSDSASDSDSGSDSDSSSDSDSESDSNSD
                     SESGSNNNVVPPNSPKNGTNASNKNEAKDSKEPLPDTGSEDEANTSLIWGLLASIGSL
                     LLFRRKKENKDKK"
     gene            3961..>4503
                     /locus_tag="O0293_002427"
     CDS             3961..>4503
                     /locus_tag="O0293_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coagulase"
                     /protein_id="HCY0281289.1"
                     /translation="MKNKLLVLSLGALCVSQIWESNRASAVVSGEKNPYVSESLKLTN
                     NKNKSRTVEEYKKSLDDLIWSFPNLDNERFDNPEYKEAMKKYQQRFMAEDEALKKFFS
                     EEKKIKNGNTDNLDYLGLSHERYESVFNTLKKQSEEFLKEIEDIKKDNPELKDFNEEE
                     QLKCDLELNKLENQILMLGKT"
BASE COUNT         1662 a          696 c          898 g         1247 t
ORIGIN      
        1 aatttatgat ttgcagttta tctaaaaatt gatttaagag ggtagttgtt tattgcgaaa
       61 aatatcattc aattttaatg aaataatggc gtcattacta taaaatatta ctttatgttg
      121 taatgcattt ttctataaga tagaactaaa aggaggggca aagatgcaaa ttagacaaat
      181 acatcaacat gactttgctc aagtggacca gttaattaga acggcatttg aaaatagtga
      241 acatggttat ggtaatgaat cagagctagt agaccaaatt cgtctaagtg atacgtatga
      301 caatacctta gaattagtag ctgttcttca aaatgaagtt gtagggcacg gtttactaag
      361 tgaagtttat cttgataacg aggcacaacg ggaaattgga ttagtgttag cacctgtatc
      421 tgttgatatt catcatcaaa ataaaggtat tgggaagcga ttgattcaag cattagaacg
      481 agaagcaata ttaaaaggat ataattttat cagtgtatta ggatggccga cgtattatgc
      541 caatctagga tatcaacgcg caagtatgta cgacatttat ccaccatatg atggtatacc
      601 agacgaagcg tttttaatta aagaattaaa agtgaacagt ttagcgggaa aaacaggtac
      661 cataaattac acatctgctt ttgaaaaaat atgatttcaa gctaggatta cattaggtag
      721 agttcatatt aataataaaa aatgtttgca atcaaatcgt acgttgtcgt ttgtaattct
      781 taaaatagca ataaataaaa tgtttgttag taaagtatta ttgtggataa taaaatatcg
      841 atacaaatta attgctataa tgcaatttta gtgtataatt ccattaacag agattaaata
      901 tatctttaaa gggtatatag ttaatataaa atgacttttt aaaaagaggg aataaaatga
      961 atatgaagaa aaaagaaaaa cacgcaattc ggaaaaaatc gattggcgtg gcttcagtgc
     1021 ttgtaggtac gttaatcggt tttggactac tcagcagtaa agaagcagat gcaagtgaaa
     1081 atagtgttac gcaatctgat agcgcaagta acgaaagcaa aagtaatgat tcaagtagcg
     1141 ttagtgctgc acctaaaaca gacgacacaa acgtgagtga tactaaaaca tcgtcaaaca
     1201 ctaataatgg cgaaacgagt gtggcgcaaa atccagcaca acaggaaacg acacaatcat
     1261 catcaacaaa tgcaactacg gaagaaacgc cggtaactgg tgaagctact actacgacaa
     1321 cgaatcaagc taatacaccg gcaacaactc aatcaagcaa tacaaatgcg gaggaattag
     1381 tgaatcaaac aagtaatgaa acgacttcta atgatactaa tacagtatca tctgtaaatt
     1441 cacctcaaaa ttctacaaat gcggaaaatg tttcaacaac gcaagatact tcaactgaag
     1501 caacaccttc aaacaatgaa tcagctccac agagtacaga tgcaagtaat aaagatgtag
     1561 ttaatcaagc ggttaataca agtgcgccta gaatgagagc atttagttta gcggcagtag
     1621 ctgcagatgc accggcagct ggcacagata ttacgaatca gttgacgaat gtgacagttg
     1681 gtattgactc tggtacgact gtgtatccgc accaagcagg ttatgtcaaa ctgaattatg
     1741 gtttttcagt gcctaattct gctgttaaag gtgacacatt caaaataact gtacctaaag
     1801 aattaaactt aaatggtgta acttcaactg ctaaagtgcc accaattatg gctggagatc
     1861 aagtattggc aaatggtgta atcgatagtg atggtaatgt tatttataca tttacagact
     1921 atgtaaatac taaagatgat gtaaaagcaa ctttgaccat gcccgcttat attgaccctg
     1981 aaaatgttaa aaagacaggt aatgtgacat tggctactgg cataggtagt acaacagcaa
     2041 acaaaacagt attagtagat tatgaaaaat atggtaagtt ttataactta tctattaaag
     2101 gtacaattga ccaaatcgat aaaacaaata atacgtatcg tcagacaatt tatgtcaatc
     2161 caagtggaga taacgttatt gcgccggttt taacaggtaa tttaaaacca aatacggata
     2221 gtaatgcatt aatagatcag caaaatacaa gtattaaagt atataaagta gataatgcag
     2281 ctgatttatc tgaaagttac tttgtgaatc cagaaaactt tgaggatgtc actaatagtg
     2341 tgaatattac attcccaaat ccaaatcaat ataaagtaga gtttaatacg cctgatgatc
     2401 aaattacaac accgtatata gtagttgtta atggtcatat tgatccgaat agcaaaggtg
     2461 atttagcttt acgttcaact ttatatgggt ataactcgaa tataatttgg cgctctatgt
     2521 catgggacaa cgaagtagca tttaataacg gatcaggttc tggtgacggt atcgataaac
     2581 cagttgttcc tgaacaacct gatgagcctg gtgaaattga accaattcca gaggattcag
     2641 attctgaccc aggttcagat tctggcagcg attctaattc agatagcggt tcagattcgg
     2701 gtagtgattc tacatcagat agtggttcag attcagcgag tgattcagat tcagcaagtg
     2761 attcagactc agcaagcgat tccgactcag cgagcgattc cgactcagac aatgactcgg
     2821 attcagatag cgattctgac tcagacagtg actcagattc cgacagtgac tcagattcag
     2881 atagcgattc tgactcagac agtgactcag attcagatag cgattcagat tcagatagcg
     2941 attcagattc cgacagtgat tccgactcag acagcgattc tgactccgac agtgattccg
     3001 actcagacag cgattcagat tccgacagtg attccgactc agatagcgat tccgactcag
     3061 atagcgactc agattcagac agcgattcag attcagacag cgattcagat tcagatagcg
     3121 attcagattc cgacagtgac tcagattccg acagtgactc ggattcagat agcgattcag
     3181 attccgacag tgactcagat tccgacagtg actcagactc agacagtgat tcggattcag
     3241 cgagtgattc ggattcagat agtgattccg actccgacag tgactcggat tcagatagcg
     3301 actcagactc ggatagcgac tcggattcag atagcgattc ggactcagat agcgattcag
     3361 aatcagacag cgattcagaa tcagacagcg attcagattc agacagcgac tcagacagtg
     3421 actcagattc agatagtgac tcggattcag cgagtgattc agactcaggt agtgactccg
     3481 attcatcaag tgattccgac tcagaaagtg attcaaatag cgattccgag tcaggttcta
     3541 acaataatgt agttccgcct aattcaccta aaaatggtac taatgcttct aataaaaatg
     3601 aggctaaaga tagtaaagaa ccattaccag atacaggttc tgaagatgaa gcaaatacgt
     3661 cactaatttg gggattatta gcatcaatag gttcattact acttttcaga agaaaaaaag
     3721 aaaataaaga taagaaataa gtaataatga tattaaatta atcatatgat tcatgaagaa
     3781 gccaccttaa aaggtggctt ttttacttgg attttccaaa tatattgttt gaatataatt
     3841 aataattaat tcatcaacag ttaattattt taaaaaggta gatgttatat aatttggctt
     3901 ggcgaaaaaa tagggtgtaa ggtaggttgt taattaggga aaattaagga gaaaatacag
     3961 ttgaaaaata aattgctagt tttatcattg ggagcattat gtgtatcaca aatttgggaa
     4021 agtaatcgtg cgagtgcagt ggtttctggg gagaagaatc catatgtatc tgagtcgttg
     4081 aaactgacta ataataaaaa taaatctaga acagtagaag agtataagaa aagcttggat
     4141 gatttaatat ggtcctttcc aaacttagat aatgaaagat ttgataatcc tgaatataaa
     4201 gaagctatga aaaaatatca acagagattt atggctgaag atgaggcttt gaagaaattt
     4261 tttagtgaag agaaaaaaat aaaaaatgga aatactgata atttagatta tctaggatta
     4321 tctcatgaaa gatatgaaag tgtatttaat actttgaaaa aacaaagtga ggagttctta
     4381 aaagaaattg aagatataaa aaaagataac cctgaattga aagactttaa tgaagaggag
     4441 caattaaagt gcgacttaga attaaacaaa ttagaaaatc agatattaat gttaggtaaa
     4501 aca
//
LOCUS       DAKTIO010000188         4499 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.193, whole genome shotgun sequence.
ACCESSION   DAKTIO010000188 DAKTIO010000000
VERSION     DAKTIO010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.193"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            126..1301
                     /gene="rpsA"
                     /locus_tag="O0293_002428"
     CDS             126..1301
                     /gene="rpsA"
                     /locus_tag="O0293_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCY0281290.1"
                     /translation="MTEEFNESMINDIKEGDKVTGEVQQVEDKQVVVHINGGKFNGII
                     PISQLSTHHIDSPSEVVKEGDEVEAYVTKVEFDEENETGAYILSRRQLETEKSYSYLQ
                     EKLDNNEIIEAKVTEVVKGGLVVDVGQRGFVPASLISTDFIEDFSVFDGQTIRIKVEE
                     LDPENNRVILSRKAVEQEENDAKKDQLLQSLNEGDVIDGKVARLTQFGAFIDIGGVDG
                     LVHVSELSHEHVQTPEEVVSIGQDVKVKIKSIDRDTERISLSIKDTLPTPFENIKGQF
                     HENDVIEGVVVRLANFGAFVEIAPGVQGLVHISEIAHKHIGTPGEVLEPGQQVNVKIL
                     GIDEENERVSLSIKATLPNEDVVESDPSTTKAYLENEEEDNPTIGDMIGDKLKNLKL"
     gene            1523..2833
                     /gene="der"
                     /locus_tag="O0293_002429"
     CDS             1523..2833
                     /gene="der"
                     /locus_tag="O0293_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019167598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCY0281291.1"
                     /translation="MTKPIVAIVGRPNVGKSTIFNRIVGERVSIVEDTPGVTRDRIYS
                     SGEWLTHDFNIIDTGGIEIGDAPFQTQIRAQAEIAIDEAYVIIFMVNVREGLTQSDEM
                     VAQILYKSKKPVVLAVNKVDNMEMRTDVYDFYSLGFGEPYPISGSHGLGLGDLLDAVV
                     SHFGEEEEDPYDEDTIRLSIIGRPNVGKSSLVNAILGEDRVIVSNVAGTTRDAIDTEY
                     SYDGQDYVLIDTAGMRKKGKVYESTEKYSVLRALKAIERSNVVLVVIDAEQGIIEQDK
                     RVAGYAHEQGKAVVIVVNKWDTVEKDSKTMKKFEDEVRKEFQFLDYAQIAFVSAKERT
                     RLRTLFPYINEASENHKKRVQSSTLNEVVTGAISMNPTPTDKGRRLNVFYATQVAIEP
                     PTFVVFVNDVELMHFSYKRYLENQIRAAFGFEGTPIHIIARKRN"
     gene            2850..3848
                     /locus_tag="O0293_002430"
     CDS             2850..3848
                     /locus_tag="O0293_002430"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCY0281292.1"
                     /translation="MTKITVFGMGSFGTALANVLAENGHDVLMWGKNQDAVDELNTCH
                     TNKKYLKYAKLDVNIIATSDMTKAIQFADIYLMALPTKAMREVASQINDKLTSKKTFI
                     HVAKGIENGTFKRVSEMIEDSISPEYNAGIGVLSGPSHAEEVVVKQPTTVAASSKDKS
                     VSKLTQDLFMNDYLRVYTNDDLIGVELGGALKNIIAVASGIVAGIGYGDNAKAALMTR
                     GLAEISRLGEKLGADPMTFLGLGGIGDLIVTCTSTHSRNFTLGYKLGQGESMDQALSE
                     MNMVVEGIYTTKSVYHLAKEKNVDMPITNALYRVLFENISVKECVKDLMERDKKSE"
     gene            4019..4291
                     /locus_tag="O0293_002431"
     CDS             4019..4291
                     /locus_tag="O0293_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCY0281293.1"
                     /translation="MNKTDLINAVAEQADLTKKEAGSAVDAVFESIQNSLAKGEKVQL
                     IGFGNFEVRERAARKGRNPQTGKEIDIPASKVPAFKAGKALKDAVK"
BASE COUNT         1614 a          601 c          901 g         1383 t
ORIGIN      
        1 atatgataaa gtgacatatt tatgtatatg actatttcgc aaaatgtaat cgaggtagaa
       61 tttcttgaca attctgtcag tttataagat gttataaata tgtagtgtat aaggaggcaa
      121 acaagatgac tgaagaattc aatgaatcaa tgattaacga tattaaagaa ggtgacaaag
      181 tcactggcga ggtacaacaa gttgaagaca agcaagttgt tgttcatatc aacggtggta
      241 aatttaatgg gattattcct attagtcaat tatctacgca tcatattgat agcccaagtg
      301 aagttgtaaa agagggcgac gaagttgaag catatgtcac taaagttgag tttgatgaag
      361 aaaatgaaac tggagcttac atcttatcta gaagacaact tgaaactgag aagtcttata
      421 gttatttaca agaaaaatta gataataatg aaatcatcga agcgaaagta acagaagtag
      481 ttaaaggtgg tttggttgtt gatgtaggac aaagaggttt tgttccggct tcactaattt
      541 caacagactt cattgaggat ttctctgtgt ttgatggaca aacaattcgt attaaagttg
      601 aagaattgga tcctgaaaat aatagagtca ttttaagccg taaagcagtt gaacaagaag
      661 aaaacgatgc taaaaaagat caattattac aatctttaaa tgaaggcgat gttattgatg
      721 gtaaagtagc gcgtttaact caatttggtg catttataga cattggcggt gttgatggtt
      781 tagtgcatgt atctgaactt tctcacgaac atgttcaaac accagaagaa gtagtttcaa
      841 ttggtcaaga tgttaaagtt aaaattaaat ctattgatag agatacagaa cgtatttcat
      901 tatcaatcaa agatacgtta ccaacacctt tcgaaaatat taaaggtcaa ttccacgaaa
      961 atgatgtcat tgaaggtgtc gtagtaagat tggcaaactt tggtgcattt gttgaaattg
     1021 caccaggtgt acaaggactt gtacatattt ctgaaattgc acacaaacac attggtacgc
     1081 caggtgaagt gttagaacct ggtcaacaag taaatgttaa aatattaggt attgatgaag
     1141 agaatgaaag agtatcacta tctattaaag caacattacc aaacgaagat gttgttgaaa
     1201 gtgatccttc tacgactaag gcgtacttag aaaacgaaga agaagataat ccaacaattg
     1261 gcgatatgat tggtgataaa cttaaaaatc ttaaactata atttaatatt taatagtcaa
     1321 ctccacatgt ttatgattgc atgtggagta tttttatgta acaaaatata ctcggaatga
     1381 taacgtggac aaatttaact aagtgtttaa aaagataaag ttttaagtgc tgatttttat
     1441 cattacagta ataaactcat tttgaataca cagtctcatg tgatattatt aaaaagatat
     1501 aagaaagaga ggaagttagc ttatgactaa acctatagta gctattgtag gtaggcctaa
     1561 tgtaggtaaa tctacaattt ttaatagaat agttggagaa cgtgtttcga ttgtggaaga
     1621 cacgccaggt gtaacacgag atcgtattta ttcttcaggt gaatggttaa cacatgattt
     1681 caatattatt gatacaggtg gtattgaaat tggtgatgca ccattccaaa cacaaattag
     1741 agcgcaggca gaaatcgcca tagatgaagc gtatgttatt atttttatgg ttaacgtgcg
     1801 tgaaggattg acacaaagcg atgaaatggt cgctcaaatt ttatacaaat ctaaaaaacc
     1861 ggtcgtatta gcggttaaca aagtagataa tatggaaatg cgtacagacg tgtatgattt
     1921 ctattcatta ggatttggtg aaccgtatcc gatatcaggg tcacatggtt taggtcttgg
     1981 tgacttgtta gatgcagttg tttctcattt tggtgaagag gaagaagatc cttatgatga
     2041 agatacaatt cgactatcca ttattggacg accaaacgta ggtaaatcaa gtttagtaaa
     2101 tgctatttta ggtgaagatc gcgttatcgt ttctaatgtt gcagggacaa cgagagacgc
     2161 tattgataca gagtatagtt atgatggaca agattatgtt ttaatcgata ctgctggtat
     2221 gcgtaaaaaa ggaaaagtat atgaatcaac tgagaaatat tcagtattaa gagctttaaa
     2281 agcgattgaa cgttcaaatg ttgttttagt ggtcatagat gcagaacaag gcatcattga
     2341 acaagataaa cgtgttgcag gatatgcaca tgaacaaggt aaagcagtcg tgattgtcgt
     2401 aaataaatgg gatactgtgg aaaaagatag taaaacgatg aagaaatttg aagatgaagt
     2461 acgtaaagaa ttccaatttt tagattatgc acaaattgct tttgtgtctg ctaaagaacg
     2521 cacaagatta cgtacattat tcccttacat caatgaagca agtgaaaacc ataaaaaacg
     2581 tgttcaaagt tcaactttaa atgaagttgt tactggtgca atttccatga accctacacc
     2641 aacagacaaa ggtagacgtt tgaatgtctt ttatgcaaca caagttgcta tagaaccacc
     2701 gacatttgtt gtatttgtta atgatgtaga attaatgcat ttttcttata aacgctattt
     2761 agagaatcaa atccgtgccg cttttggttt tgaaggtaca ccaattcata ttatagctcg
     2821 aaagagaaat taacgattgg gggataacaa tgactaaaat taccgttttt ggtatgggaa
     2881 gttttgggac agcccttgcc aatgttcttg cagaaaatgg acatgatgtt ttgatgtggg
     2941 gtaaaaatca agatgctgtt gatgaattaa atacatgtca tacaaataaa aagtatttaa
     3001 aatacgcgaa attagatgtt aacatcatcg ctacttcaga tatgaccaaa gcaattcaat
     3061 ttgcagatat ttacttaatg gctttaccta ctaaagcaat gcgagaagtt gcttctcaaa
     3121 ttaatgataa gctgacctct aaaaagactt ttatacatgt tgctaaaggt attgaaaatg
     3181 ggacgtttaa acgtgtgtca gaaatgattg aagattctat ttcacctgaa tataatgcag
     3241 gtattggcgt gttgtcaggg ccaagtcatg cggaagaagt tgtagtcaag caaccaacta
     3301 cagttgctgc ttcatcaaaa gataaaagtg taagtaaatt aacgcaagat ttatttatga
     3361 atgattattt gcgtgtgtac acgaatgatg acttgattgg tgttgaactt ggtggtgcat
     3421 tgaaaaatat catcgcagta gcaagtggta tcgtagctgg aattggctac ggtgataatg
     3481 caaaagctgc attaatgact cgtggcttag cggaaattag tagattaggt gaaaagttag
     3541 gtgccgatcc tatgacattt ctaggtttag gtggtatcgg tgacttaatc gttacttgca
     3601 catcaacaca ttctcggaat ttcacattag gatataaact tggacaaggt gaatcaatgg
     3661 atcaagcatt atctgaaatg aatatggttg ttgaaggtat ttatacaact aaatcagttt
     3721 atcatttagc taaagaaaaa aatgtggata tgccaattac aaatgcatta tatagagtat
     3781 tatttgaaaa tatctcagta aaagaatgcg taaaagattt aatggagcgc gataaaaaat
     3841 ctgaataaaa tgtgtattat tacacatata cggctaataa ttacgtaaaa tgaatagaaa
     3901 tggcttaaat tcaacgtttt tgccacataa atgattgcga tttatgtaat gcttgtgtta
     3961 aagttttacc ataatgatat aaacatatca ttatcaaacc tcaggaggtg aatgtctaat
     4021 gaacaaaaca gatttaatca atgcagttgc agagcaagct gatttaacta aaaaagaagc
     4081 tggttcagca gtagatgctg tattcgaatc aatccaaaac tcacttgcta aaggtgaaaa
     4141 agtacaatta attggtttcg gtaactttga ggtacgtgaa cgtgctgcac gtaaaggtcg
     4201 taaccctcaa actggtaaag aaattgatat cccagcaagt aaagttccag cattcaaagc
     4261 tggtaaagca ttaaaagatg ctgtaaaata attttactta aaaagccctg aataagggct
     4321 ttttattttg cttttaatac ttacaacctg tacataaatt gtaatgttct tctaagtttt
     4381 taatctttgg aacattcatt gtttaagcgt tcaaaatatt acatgacata atagttttga
     4441 taaaaatagc aactacaaaa tatacatatg aatattgaga ttaattgtta gcgttgaat
//
LOCUS       DAKTIO010000189         4473 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.194, whole genome shotgun sequence.
ACCESSION   DAKTIO010000189 DAKTIO010000000
VERSION     DAKTIO010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.194"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            50..4195
                     /gene="sdrD"
                     /locus_tag="O0293_002432"
     CDS             50..4195
                     /gene="sdrD"
                     /locus_tag="O0293_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrD"
                     /protein_id="HCY0281294.1"
                     /translation="MLNRENKTAITRKGMVSNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAESTNKELNEATTSASDNQSSDKVDMQQLNQEDNTKNDNQKEMVSSQGNE
                     TTSNGNKLIEKESVQSTTGNKVEVSTAKSDEQASPKSTNEDLNTKQTISNQEALQPDL
                     QENKSVVNVQPTNEENKKVDAKTESTTLNVKSDAIKSNDETLVDNNSNSNNENNADII
                     LPKSTAPKRLNTRMRIAAVQPSSTEAKNVNDLITSNTTLTVVDADKNNKIVPAQDYLS
                     LKSQITVDDKVKSGDYFTIKYSDTVQVYGLNPEDIKNIGDIKDPNNGETIATAKHDTA
                     NNLITYTFTDYVDRFNSVQMGINYSIYMDADTIPVSKNDVEFNVTIGNTTTKTTANIQ
                     YPDYVVNEKNSIGSAFTETVSHVGNKENPGYYKQTIYVNPSENSLTNAKLKVQAYHSS
                     YPNNIGQINKDVTDIKIYQVPKGYTLNKGYDVNTKELTDVTNQYLQKITYGDNNSAVI
                     DFGNADSAYVVMVNTKFQYTNSESPTLVQMATLSSTGNKSVSTGNALGFTNNQSGGAG
                     QEVYKIGNYVWEDTNKNGVQELGEKGVGNVTVTVFDNNTNTKVGEAVTKEDGSYLIPN
                     LPNGDYRVEFSNLPKGYEVTPSKQGNNEELDSNGLSSVITVNGKDNLSADLGIYKPKY
                     NLGDYVWEDTNKNGIQDQDEKGISGVTVTLKDENGNVLKTVTTDADGKYKFTDLDNGN
                     YKVEFTTPEGYTPTTVTSGSDIEKDSNGLTTTGVINGADNMTLDSGFYKTPKYNLGNY
                     VWEDTNKDGKQDSTEKGISGVTVTLKNENGEVLQTTKTDKDGKYQFTGLENGTYKVEF
                     ETPSGYTPTQVGSGTDEGIDSNGTSTTGVIKDKDNDTIDSGFYKPTYNLGDYVWEDTN
                     KNGVQDKDEKGISGVTVTLKDENDKVLKTVTTDENGKYQFTDLNNGTYKVEFETPSGY
                     TPTSVTSGNDTEKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQ
                     DSTEKGIKDVKVTLLNEKGEVIGTTKTDENGKYCFDNLDSGKYKVIFEKPAGLTQTGT
                     NTTEDDKDADGGEVDVTITDHDDFTLDNGYYEEETSDSDSDSDSDSDSDRDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDRDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DAGKHTPVKPMSTTKDHHNKAKALPETGNENSGSNNATLFGGLFAALGSLLLFGRRKK
                     QNK"
BASE COUNT         1833 a          688 c          795 g         1157 t
ORIGIN      
        1 tttaaaacaa ttgtattatt taaaaatttg atgaattagg agtaatctaa tgctaaacag
       61 agaaaataaa acggcaataa caaggaaagg catggtatcc aatcgattaa ataaattttc
      121 gattagaaag tacacagtgg gaacagcatc aattttagta ggtacaacat taatttttgg
      181 tctggggaac caagaagcaa aggctgcaga aagtactaat aaagaattga acgaagcgac
      241 aacttcagca agtgataatc aatcgagtga taaagttgat atgcagcaac taaatcaaga
      301 agacaatact aaaaatgata atcaaaaaga aatggtatca tctcaaggta atgaaacgac
      361 ttcaaatggg aataaattaa tagaaaaaga aagtgtacaa tctaccactg gaaataaagt
      421 tgaagtttca actgccaaat cagatgagca agcttcacca aaatctacga atgaagattt
      481 aaacactaaa caaactataa gtaatcaaga agcgttacaa cctgatttgc aagagaataa
      541 atcagtggta aatgttcaac caactaatga ggaaaacaaa aaggtagatg ccaaaactga
      601 atcaactaca ttaaatgtta aaagtgatgc tatcaagagt aatgatgaaa ctcttgttga
      661 taacaatagt aattcaaata atgaaaataa tgcagatatc attttgccaa aaagtacagc
      721 acctaaacgt ttgaatacaa gaatgcgtat agcagcagta cagccatcat caacagaggc
      781 taaaaatgtt aatgatttaa tcacatcaaa tacaacatta actgtcgttg atgcagataa
      841 aaacaataaa atcgtaccag cccaagatta tttatcatta aaatcacaaa ttacagttga
      901 tgacaaagtt aaatcaggtg attatttcac aattaaatac tcagatacag tacaagtata
      961 tggattgaat ccggaagata ttaaaaatat tggtgatatt aaagatccaa ataatggtga
     1021 aacaattgcg actgcaaaac atgatactgc aaataattta attacatata catttacaga
     1081 ttatgttgat cgatttaatt ctgtacaaat gggaattaat tattcaattt atatggatgc
     1141 tgatacaatt cctgttagta aaaacgatgt tgagtttaat gttacgatag gtaatactac
     1201 aacaaaaaca actgctaaca ttcaatatcc agattatgtt gtaaatgaga aaaattcaat
     1261 tggatcagcg ttcactgaaa cagtttcaca tgttggaaat aaagaaaatc cagggtacta
     1321 taaacaaacg atttatgtaa atccatcgga aaattcttta acaaatgcca aactaaaagt
     1381 tcaagcttac cactcaagtt atcctaataa tatcgggcaa ataaataaag atgtaacaga
     1441 tataaaaata tatcaagttc ctaaaggtta tacattaaat aaaggatacg atgtgaatac
     1501 taaagagctt acagatgtaa caaatcaata cttgcagaaa attacatatg gcgacaacaa
     1561 tagcgctgtt attgattttg gaaatgcaga ttctgcttat gttgtaatgg ttaatacaaa
     1621 attccaatat acaaatagcg aaagcccaac acttgttcaa atggctactt tatcttcaac
     1681 aggtaataaa tccgtttcta ctggcaatgc tttaggattt actaataacc aaagtggcgg
     1741 agctggtcaa gaagtatata aaattggtaa ctacgtatgg gaagatacta ataaaaacgg
     1801 tgttcaagaa ttaggagaaa aaggcgttgg caatgtaact gtaactgtat ttgataataa
     1861 tacaaataca aaagtaggag aagcagttac taaagaagat gggtcatact tgattccaaa
     1921 cttacctaat ggagattacc gtgtagaatt ttcaaactta ccaaaaggtt atgaagtaac
     1981 cccttcaaaa caaggtaata acgaagaatt agattcaaac ggcttatctt cagttattac
     2041 agttaatggc aaagataact tatctgcaga cttaggtatt tacaaaccta aatacaactt
     2101 aggtgactat gtctgggaag atacaaataa aaatggtatc caagaccaag atgaaaaagg
     2161 tatatctggc gtaacggtaa cattaaaaga tgaaaacggt aacgtgttaa aaacagttac
     2221 aacagacgct gatggcaaat ataaatttac tgatttagat aatggtaatt ataaagttga
     2281 atttactaca ccagaaggct atacaccgac tacagtaaca tctggtagcg acattgaaaa
     2341 agactctaat ggtttaacaa caacaggtgt tattaatggt gctgataaca tgacattaga
     2401 tagtggattc tacaaaacac caaaatataa tttaggtaat tatgtatggg aagatacaaa
     2461 taaagatggt aagcaggatt caactgaaaa aggtatttca ggcgtaacag ttacattgaa
     2521 aaatgaaaac ggtgaagttt tacaaacaac taaaacagat aaagatggta aatatcaatt
     2581 tactggatta gaaaatggaa cttataaagt tgaattcgaa acaccatcag gttacacacc
     2641 aacacaagta ggttcaggaa ctgatgaagg tatagattca aatggtacat caacaacagg
     2701 tgtcattaaa gataaagata acgatactat tgactctggt ttctacaaac cgacttacaa
     2761 cttaggtgac tatgtatggg aagatacaaa taaaaacggt gttcaagata aagatgaaaa
     2821 gggcatttca ggtgtaacag ttacgttaaa agatgaaaac gacaaagttt taaaaacagt
     2881 tacaacagat gaaaatggta aatatcaatt cactgattta aacaatggaa cttataaagt
     2941 tgaattcgag acaccatcag gttatacacc aacttcagta acttctggaa atgatactga
     3001 aaaagattct aatggtttaa caacaacagg tgtcattaaa gatgcagata acatgacatt
     3061 agacagtggt ttctataaaa caccaaaata tagtttaggt gattatgttt ggtacgacag
     3121 taataaagac ggcaaacaag attcaactga aaaaggtatc aaagatgtta aagttacttt
     3181 attaaatgaa aaaggcgaag taattggaac aactaaaaca gatgaaaatg gtaaatactg
     3241 ctttgataat ttagatagcg gtaaatacaa agttattttt gaaaagcctg ctggcttaac
     3301 acaaacaggt acaaatacaa ctgaagatga taaagatgca gatggtggcg aagttgacgt
     3361 aacaattacg gatcatgatg atttcacact tgataatgga tactacgaag aagaaacatc
     3421 agatagcgac tcagattcgg acagcgactc agattcagac agagactcag actcagatag
     3481 tgattcagac tcggatagcg attcagattc agacagcgat tcagattcag atagcgattc
     3541 agattcagac agagactcag atagtgattc agactcagat agcgactcag attcagacag
     3601 cgactcagat tcagacagcg actcagactc agatagtgat tcagactcag atagcgactc
     3661 agattcagac agcgactcag actcagacag cgactcagac tcagatagtg actcagattc
     3721 agatagcgac tcagattcgg acagcgattc agactcagat agcgactcag attcagatag
     3781 cgattcggac tcagatagcg actcagattc agatagtgat tcagactcag atagcgactc
     3841 agattcagac agcgactcag attcggatag cgactcagat tcagacagcg actcagactc
     3901 ggatagtgat tcagactcag atagcgactc agactcagat agcgattcag attcagatag
     3961 cgactcagac tcagacagcg attcagactc agacagcgac tcagactcag atgcaggtaa
     4021 gcacacacct gttaaaccaa tgagtactac taaagaccat cacaataaag caaaagcatt
     4081 accagaaaca ggtaatgaaa atagcggctc aaataacgca acgttatttg gcggattatt
     4141 cgcagcatta ggatcattat tgttattcgg tcgtcgtaaa aaacaaaata aataatacag
     4201 cttataccag gtccgtgagg cctggttttt tatttcaaaa tttatgaaat gtatcttatg
     4261 tttgagatta aatagtgcaa ttttttaaaa aatataacat aaatcaacat aaatttaatt
     4321 catctttaca ttaaaagtac aaacgaataa gaaaagcaat taaacacagt atcaaaccat
     4381 ttaaaacaat tgatataaaa aacaaacgaa taattaaaaa gtactagaat taatttattc
     4441 aaaccaaagt gtaaaataac agtcttagat aaa
//
LOCUS       DAKTIO010000190         4467 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.195, whole genome shotgun sequence.
ACCESSION   DAKTIO010000190 DAKTIO010000000
VERSION     DAKTIO010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.195"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..96
                     /locus_tag="O0293_002433"
     CDS             <1..96
                     /locus_tag="O0293_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281295.1"
                     /translation="IIWKLKRLQKIVTSFITKHESTSIGLVENYF"
     gene            complement(194..1108)
                     /gene="rbsK"
                     /locus_tag="O0293_002434"
     CDS             complement(194..1108)
                     /gene="rbsK"
                     /locus_tag="O0293_002434"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCY0281296.1"
                     /translation="MTNKVVILGSTNVDQFLTVERYAQPGETLHVEEAQKAFGGGKGA
                     NQAIATARMQADTTFITKIGTDGVADFILEDFKVAHIDTSYIIKTAEAKTGQAFITVN
                     AEGQNTIYVYGGANMTMTPEDVINAKDAIINADFVVAQLEVPIPAIISAFEIAKAHGV
                     TTVLNPAPAKALPNELLSLIDIIVPNETEAELLSGIKVTNEQSMKDNANYFLSIGIKT
                     VLITLGKQGTYFATKNQSQHIEAYKVNAIDTTAAGDTFIGAFVSRLNKSQDNLADAID
                     FGNKASSLTVQKHGAQASIPLLEEVNQV"
     gene            complement(1136..1540)
                     /gene="rbsD"
                     /locus_tag="O0293_002435"
     CDS             complement(1136..1540)
                     /gene="rbsD"
                     /locus_tag="O0293_002435"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCY0281297.1"
                     /translation="MKKSAILNEHISKAIATIGHFDLLTINDAGMPIPNDHRRIDLAV
                     TKNLPRFIDVLATVLEEMEIQKIYLAEEIKEHNPTQLQQIKQLISSEIEIIFIPHEEM
                     KSNLAHPLNKGNIRTGETTPYSNIALESNVTF"
     gene            complement(1555..2436)
                     /gene="rbsU"
                     /locus_tag="O0293_002436"
     CDS             complement(1555..2436)
                     /gene="rbsU"
                     /locus_tag="O0293_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose transporter RbsU"
                     /protein_id="HCY0281298.1"
                     /translation="MSIVALLIGLGPLIGWGFFPTVASKFGGKPVHQIIGATVGTLIF
                     AIILAVVTSSGLPTGTNLLFALLSGAGWGFGQIITFKAFELVGSSRAMPVTTAFQLLG
                     ASLWGVFALGNWPGIGHKIIGFTALVVILIGARMTVWSERKEASNAKNLRRAVVLLLI
                     GEFGYWLYSAAPQATSIDGLTAFLPQAMGMVIVAVIYGFMNMKAENPFRNKITWLQII
                     SGFFFAFGALTYLISAQPNMNGLATGFILSQTSVVLATLTGIYFLKQHKTSKEMVITI
                     IGLVLILVAASVTVFIK"
     gene            complement(2668..3666)
                     /locus_tag="O0293_002437"
     CDS             complement(2668..3666)
                     /locus_tag="O0293_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCY0281299.1"
                     /translation="MKKVSIKDVAREAGVSVTTVSHILNHNDSRFSATTIKNVHAVSE
                     RLGYAPNKHAKQLRGSKIQTIGVILPSLTNPFFSALMQSIHDHKPSDVDLCFLTSTAT
                     DLYDNIKHLIDRGIDGLIIAQYISSPDALNNYLKKHHVPYVVLDQNDHQGYTDFVRTN
                     EYQGGQLAAQHLIELGHNHMIIVAPYDMMANMSTRVAGFVDTLRANQLPEPQIVHTEL
                     SKRGGLTIVDDIMVQSATAIFAINDELAIGILRGLIEHGISIPKDISLIGYDDIDYAA
                     YVSPPLTTVAQPITDIGKTSLTLLLQRLQHLDKSIDMIELPTTLKIRATTGYHLSN"
     gene            3817..3996
                     /locus_tag="O0293_002438"
     CDS             3817..3996
                     /locus_tag="O0293_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCY0281300.1"
                     /translation="MRRENILLRLIPFNDYDNKNFKMFIVIKNTKVIILKNKIFVFEQ
                     SIKKHNEMMYIELCD"
     gene            4046..4438
                     /locus_tag="O0293_002439"
     CDS             4046..4438
                     /locus_tag="O0293_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281301.1"
                     /translation="MQFKLKEEEIISFLELKYPEKEFEYGRLLVGQHKRDDLDVYYFG
                     DTFLMCTIISFKTFEIKETVELSYDAVNRIVLKDGWLFRKMRIETMQKVLKYGTSKLM
                     LTDFQKENYNKYIQGQKQRVIFENGHFV"
BASE COUNT         1451 a          708 c          809 g         1499 t
ORIGIN      
        1 ataatttgga agttaaaaag attacaaaaa attgtcactt catttataac taaacatgag
       61 tcaacaagca taggtttagt ggaaaattat ttctaataga tgatgtttta taaagataaa
      121 aaagcacagc catgatatac gaatgttgca tgttatatgc taaaccttca tatcatagct
      181 gtgtttgatt catttaaact tgatttactt cttctagtag aggaatagat gcttgcgcgc
      241 cgtgtttttg tacagtgagt gagctcgctt tattaccaaa atcaatagca tctgctaagt
      301 tatcttgcga cttgtttaag cgactgacaa atgcaccaat aaatgtgtcg cctgcagcag
      361 ttgtatcaat cgcatttact ttataagctt cgatgtgttg gctttgattt ttagtagcaa
      421 aatatgtacc ttgcttacct agcgtaatca aaacagtctt aatgcctata gataaaaagt
      481 aattggcatt gtctttcata gattgttcat tagttacttt aatcccagat aacaattcgg
      541 cttctgtttc gtttggcaca ataatatcga ttaatgataa taattcatta ggtaatgctt
      601 tcgctggtgc aggatttaat actgtcgtca caccatgtgc cttggcaatt tcaaatgcag
      661 atataatagc cgggatgggt acttctaatt gtgcaacgac aaagtctgca ttgattatag
      721 cgtcttttgc gttaataaca tcttcaggtg tcatcgtcat attcgcacca ccataaacat
      781 agatggtgtt ttgtccttct gcattcacag tgataaaggc ttggcccgtt tttgcttcag
      841 ctgttttgat aatatatgat gtatcaatat gagctacttt aaaatcttct aagatgaaat
      901 cagcaacgcc atcagtgcca attttagtaa taaatgttgt gtctgcttgc atgcgtgcag
      961 tggcaatagc ctggttggca cctttacctc cgccgaatgc tttttgtgct tcttcaacat
     1021 gtaatgtttc gcctggttgt gcatatcttt caactgttaa aaattgatcg acattcgttg
     1081 aacctaaaat aacaactttg ttggtcatgt gtacgctcct ttcaagttat aacttttaaa
     1141 aagtaacatt cgattctaat gcaatattag agtagggcgt tgtttcacca gtacgaatat
     1201 tacctttatt taatgggtga gctaagttac ttttcatttc ttcgtgagga atgaaaatga
     1261 tttcgatttc cgatgaaatc aattgtttaa tttgttgcaa ttgtgtaggg ttatgttctt
     1321 ttatttcttc tgctaagtat attttttgga tttccatttc ttctaacact gtagctaaga
     1381 catcaataaa gcgtggtaag tttttagtta cagctaggtc gatacgacga tgatcatttg
     1441 gaattggcat gccagcgtca ttaatcgtta ataaatcaaa atgaccaatt gtcgcgattg
     1501 cttttgaaat atgttcattt aaaatagctg attttttcat gacatctaca ctccttattt
     1561 tataaatact gtaacagaag cggctactaa aatgagtact aagccgatga ttgtaataac
     1621 catttctttt gacgttttat gttgttttaa gaaataaata ccagttaatg tagcaagcac
     1681 aacggatgtt tgagaaagaa taaatccagt tgctaaacca ttcatattag gttgtgctga
     1741 aataagatat gttaaagcac caaatgcaaa gaagaaacct gaaataattt gtaaccacgt
     1801 aattttatta cggaatggat tctctgcttt catattcata aagccataaa tgactgcaac
     1861 aattaccata cccattgctt gaggtaaaaa ggcagttagg ccatcaatag aagttgcttg
     1921 cggtgcagct gaatataacc agtatccaaa ttcaccaatt aacagaagta ccactgcacg
     1981 acgtaaattt ttggcgttac ttgcttcttt gcgttcactc caaactgtca tacgcgctcc
     2041 aattagaata acgactaaag ctgtaaatcc aatgatttta tgaccaatgc ctggccaatt
     2101 tcctaatgca aagacacccc ataaagatgc gcctaataat tggaatgctg ttgtgactgg
     2161 catggcacga gatgagccga ctaattcgaa cgctttaaat gtaatgattt gtccgaatcc
     2221 ccatcctgca cctgataata aggcgaatag caaattggtt ccagtaggta agccacttga
     2281 tgtgactacg gctaatataa tagcgaagat taacgtacct acagtagcac cgataatttg
     2341 atgtacaggt ttaccaccaa actttgaagc gactgttggg aagaagcccc agccaattaa
     2401 ggggcctaac ccgataagta atgcaacaat gctcatgtta tacacctcat gatttgtttt
     2461 tagtaaaacg ttttaccagt gccatcgtac catttttatt tgtaaaatgc atgcgttttc
     2521 ataaaaactg aaaacgttta aatcatattt aaaaattcat acattattat tttaatttct
     2581 tagtaaaata attatgatta aaaaccctca gcattaaagc aacgctaagg gcctaacaat
     2641 gatgagtata tttcggaaga tacgtagtta gtttgaaaga tgatagccag ttgttgcacg
     2701 aatttttaaa gtcgttggta attcaatcat atcaatggat ttatctaagt gctgtaatcg
     2761 ttgaagtaat aaggttaaag atgttttgcc aatatcagtt ataggttgtg ccacagtagt
     2821 taaaggtggc gagacgtacg ctgcataatc aatgtcgtca taacctatta atgagatatc
     2881 tttcgggata ctgatgccat gttcaattag tcctcgtaaa atgccaatag cgagttcatc
     2941 gttaatagcg aagattgcag tggcagattg aaccatgatg tcatcaacaa tggttagccc
     3001 accgcgctta gataattcag tatggacgat ttgtggttct ggcaattgat tcgcgcgcaa
     3061 agtatcgaca aatccagcga cacgagtcga catattcgcc atcatgtcat atggtgcaac
     3121 aattatcata tggttgtgac cgagttctat taaatgttgt gctgcaagtt gtccaccttg
     3181 atattcattt gtccgaacaa aatctgtata gccttgatgg tcattttgat ccagtacgac
     3241 ataaggtaca tgatgtttct ttagatagtt atttagggcg tccggggatg atatgtattg
     3301 tgcgataatt aatccgtcaa tacctcgatc aattaaatgt ttaatattgt catacaaatc
     3361 agttgctgta gatgttaaaa agcataaatc aacatcagat ggtttatggt catgaatact
     3421 ttgcatcagt gctgagaaaa acggatttgt taagctaggc aaaatgacgc caatagtttg
     3481 aattttactg ccgcgcaatt gttttgcatg tttattaggg gcatagccta aacgttctga
     3541 aacagcatgt acgtttttta tcgttgttgc ggaaaaacga ctatcattat gatttaaaat
     3601 atgtgacaca gttgtaactg atacaccagc ttctctagca acatctttaa ttgacacttt
     3661 tttcatatta tattccttct ctttattgtg taattaatca tacagttata tacccgatta
     3721 ttatattcat catatcatga taaacatttg atgacatatg aagacgtgaa tgacttttat
     3781 tgtctacaaa cagacgtctg aagtatacga ttaaggttga gacgagagaa tatattatta
     3841 aggttaatac cttttaatga ttatgataac aagaatttta aaatgtttat tgtgattaaa
     3901 aatacaaaag tcattatttt aaagaataaa atatttgttt ttgaacaaag tattaaaaaa
     3961 cataacgaaa tgatgtatat tgaactatgt gattgaaaaa atttttaatt aaggcttgtt
     4021 aaaacttaag agaggatgtt tttaaatgca attcaaatta aaagaagaag agattattag
     4081 ttttttagaa ttgaaatatc cagaaaaaga gttcgaatat ggtcgtttgt tagttggaca
     4141 acataaacgt gatgatttag atgtttatta ctttggtgat acgtttttaa tgtgcacgat
     4201 tatttcattc aagacatttg aaattaaaga aacagtagaa ttatcatatg atgctgttaa
     4261 tcgtattgtg ttaaaagatg gatggttatt tagaaaaatg agaatagaaa caatgcaaaa
     4321 agtgttaaaa tacggtacat ctaaattaat gttaactgat tttcaaaaag agaattataa
     4381 taaatatatt caaggtcaga aacaacgcgt gatatttgaa aatggccatt ttgtctaatt
     4441 gatagtgaat ataattagag taagagg
//
LOCUS       DAKTIO010000191         4398 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.196, whole genome shotgun sequence.
ACCESSION   DAKTIO010000191 DAKTIO010000000
VERSION     DAKTIO010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.196"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            74..1771
                     /locus_tag="O0293_002440"
     CDS             74..1771
                     /locus_tag="O0293_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HCY0281302.1"
                     /translation="MAYDGLFTKKMVESLQFLTTGRVHKINQPDNDTILMVVRQNRQN
                     HQLLLSIHPNFSRLQLTTKKYDNPFNPPMFARVFRKHLEGGIIESIKQIGNDRRIEID
                     IKSKDEIGDTIYRTVILEIMGKHSNLILVDENRKIIEGFKHLTPNTNHYRTVMPGFNY
                     EAPPTQHKINPYDITGAEVLKYIDFNAGNIAKQLLNQFEGFSPLITNEIVSRRQFITS
                     STLPEAFDEVMAETKLPPTPIFHKNHETGKEDFYFIKLNQFNDDTVTYDSLNDLLDRF
                     YDARGERERVKQRANDLVRFVQQQLYKYQNKLAKLIEEYEQSKNKDTEQLYGELITAN
                     IYRIKQGDKEVTALNYYTNEEVVIPLNPTKSPSANAQYYYKQYNRMKTRERELQHQIQ
                     LTKDNIDYFSTIEQQLHHISVHDIDEIRDELAEQGFMKQRKNQTKKKKAQIQLQHYVS
                     TDGDDIYVGKNNKQNDYLTNKKAKKTHTWLHTKDIPGSHVVIFNDAPSDTTIKEAAML
                     AGYFSKAGNSGQIPVDYTLIKNVHKPSGAKPGFVTYDNQKTLYATPDYELIQKMKQS"
     gene            complement(2034..2435)
                     /locus_tag="O0293_002441"
     CDS             complement(2034..2435)
                     /locus_tag="O0293_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0281303.1"
                     /translation="MDIPKITTFLMFNNQAEEAVKLYTSLFEDSEIITMAKYGENGPG
                     DPGTVQHSIFTLNGQVFMAIDANSGTELPMNPAISLFVTVKDTIEMERLFNGLKDEGA
                     ILMPKTNMPPYREFAWVQDKFGVSFQLALPE"
     gene            complement(2872..3084)
                     /locus_tag="O0293_002442"
     CDS             complement(2872..3084)
                     /locus_tag="O0293_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281304.1"
                     /translation="MNDKTSNDLYGKIKHCNEFINHSNDSNLSSSHDVDESSTKQKHI
                     KNKTTIDHNDDLFKHVKDILRKQEQI"
     gene            complement(3114..3725)
                     /locus_tag="O0293_002443"
     CDS             complement(3114..3725)
                     /locus_tag="O0293_002443"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCY0281305.1"
                     /translation="MAKEIAKSLLDIEAVTLSPNDLYTWSSGIKSPIYCDNRVTLGYP
                     LVRGAIRDGLINLIKEHFPEVEVISGTATAGIPHAAFIAEKLKLPMNYVRSSNKSHGK
                     QNQIEGAKSEGKKVVVIEDLISTGGSSVTAVEALKLAGAEVLGVVAIFTYGLKKADDT
                     FSNIQLPFYTLSDYNELIEVAENEGKISSEDIQTLVEWRDNLA"
     gene            complement(3725..>4398)
                     /gene="pyrF"
                     /locus_tag="O0293_002444"
     CDS             complement(3725..>4398)
                     /gene="pyrF"
                     /locus_tag="O0293_002444"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCY0281306.1"
                     /translation="ALDFESKEKVNQFLDLFDESLFVKVGMELFYQEGPQLINEIKER
                     GHDVFLDLKLHDIPNTVGKAMEGLAKLNVDLVNVHAAGGVKMMSEAIKGLRKHNQDTK
                     IIAVTQLTSTTEDMLRHEQNIQTSIEEAVLNYAKLANAAGLDGVVCSPLESRMLTEKL
                     GTSFLKVTPGIRPKGASQNDQHRITTPEEARQLGSTHIVVGRPITQSDNPVESYHKIK
                     ESWLV"
BASE COUNT         1510 a          728 c          663 g         1497 t
ORIGIN      
        1 tcaataacta tactgaaaag ttacaatcat gttaaaatga aacgaatgat atgaagaagg
       61 tggaagataa attatggctt atgatggctt atttacaaag aaaatggttg agtctctaca
      121 atttttaaca acaggacgcg ttcacaaaat caatcaacct gataatgaca cgatactaat
      181 ggttgtacgt caaaatagac aaaaccatca attgttattg tcaatccatc caaacttttc
      241 aagattacaa ttgactacta aaaaatatga taatccattt aatccaccca tgtttgcgcg
      301 tgtttttaga aaacacttag aaggtggtat tatcgaatcg attaagcaaa ttggtaatga
      361 tcgtcgcatt gaaatcgata taaagagtaa agatgaaatt ggcgatacta tttaccgcac
      421 tgtcatcctt gagattatgg gtaaacatag taacttaatt ttagtagatg aaaatcgcaa
      481 aataattgaa ggatttaaac acttaacacc aaatacgaat cactatcgta cagtaatgcc
      541 aggatttaat tatgaagcac cacctactca gcacaaaata aatccgtatg atattacagg
      601 tgcagaggtg ttgaaatata tcgattttaa cgcaggtaat attgctaaac aattattgaa
      661 tcagtttgaa ggatttagcc ctttaattac gaatgaaatc gttagtcgtc gtcaatttat
      721 tacttcatca acattaccag aagcatttga cgaagtaatg gcagaaacca agttaccacc
      781 tactcctatt tttcataaaa atcatgaaac aggtaaagag gatttctatt ttataaagtt
      841 aaatcaattt aatgatgata cagttacata cgattcatta aatgatttgc ttgatcgttt
      901 ttatgatgcg cgtggcgaac gtgaacgcgt taaacaacgt gcgaatgatt tagttcgatt
      961 tgttcaacag cagttgtaca aatatcaaaa taaattagcg aagttgattg aagaatatga
     1021 gcagtctaaa aataaagata ctgaacagtt atatggtgaa ttgatcactg ctaatatata
     1081 tcgaattaag caaggcgata aagaagtgac ggcattgaat tattatacga atgaagaagt
     1141 tgtcattcct ttaaatccta caaaatcccc atcagcaaat gctcaatatt attataaaca
     1201 atataatcgt atgaaaacga gagaacgtga attacaacat caaattcaat tgacgaaaga
     1261 caatatagat tatttttcaa caatcgaaca acaattacat catatttctg tccatgacat
     1321 tgatgaaatt agagatgaat tagcagaaca aggctttatg aaacagcgta aaaatcaaac
     1381 taagaaaaag aaagcgcaga ttcaattaca acattatgta tcaactgatg gcgacgatat
     1441 atatgttggt aagaataaca agcaaaatga ttatttaaca aataaaaaag ctaaaaaaac
     1501 tcacacatgg ttacacacaa aagatattcc tggttcacat gtcgttatat ttaatgatgc
     1561 accaagtgat acgacaatca aggaagcggc tatgttagca ggatactttt caaaagctgg
     1621 taattctgga caaatacctg ttgattatac attaattaaa aatgtgcata aaccatcagg
     1681 tgcaaagcct gggtttgtaa catatgacaa tcaaaaaact ttgtatgcta cacctgatta
     1741 tgaacttatt caaaaaatga aacaatcata atcaagttgt cgacaaataa acacctcaca
     1801 aaagaaagta gtcctttctt atggtgaggt gtttatttat tatttaatgc gttcattgaa
     1861 acgaagtatc gtgtttttaa ataacgtatc atcatttaag ttacaaatta tctttgtgat
     1921 tttaaaaatg agtgaaacta aaatgctaat gtgtacttaa aatagaaata cataggtaaa
     1981 acgcatgcat aagaaaaagt gtttctttat cattcaacgc tgtgcgaatc cttttactca
     2041 ggtaatgcta attgaaaact tactccaaac ttatcttgaa cccaagcaaa ctctctgtat
     2101 ggtggcatat tcgtttttgg cattaaaatg gcaccttcat cttttaatcc attaaatagt
     2161 cgttccattt caatagtatc ttttactgta acaaataatg aaatcgcagg attcattggt
     2221 aattctgtgc cactattagc atcaatcgcc atgaatactt gtccatttaa tgtaaatatt
     2281 gagtgttgta cagtcccggg atcaccaggt ccattttcac catacttagc cattgttata
     2341 atctcactat cttcaaataa gcttgtgtat agtttaacag cttcttcagc ttggttatta
     2401 aacattaaaa atgtcgtgat ttttggaata tccatgctat gcctcctata gattgaactt
     2461 ctataactac tattatctta acataattaa aatgacaaaa gcgcgtcgta ccgttatgtg
     2521 cacgaatgac aaacgtctta ttggcctgtt gattaaatca catagttaac gataaaaatt
     2581 acgcaagatt aaaaatatga tacatatgtt aataaatgat acttatcata ggtattaatg
     2641 ttttgactta ttgtcaaatt tgattacaat taaaattaag ttaaaggagg gacgttatgt
     2701 tacattatgt gattccagta ttagtcctat tactcgtatt cttaattggt tggattttaa
     2761 taaagttaat tatccgctaa taaatttttt atataataaa agctatagtg tttgtaatat
     2821 tcggatgcgg ttaagcagcc cctgatatta aacactatag cttttttgtt tttaaatttg
     2881 ttcttgttta cgtaatatat cctttacatg tttaaataaa tcatcattat gatctatagt
     2941 tgttttattt tttatatgtt tttgcttcgt tgaactttcg tcgacatcgt gactactaga
     3001 tagattggaa tcatttgaat gattgataaa ttcgttacaa tgttttatct tcccatataa
     3061 atcattagat gttttgtcat tcatttgtcg aattcctcct tctagtgtct atattatgct
     3121 aagttgtctc tccattcaac taatgtttgg atatcttcac tagaaatttt accttcattt
     3181 tcagctactt caattaattc attgtaatca cttaaagtgt aaaaaggtag ttgaatattg
     3241 ctaaatgtat catctgcttt tttcaaaccg taagtaaaga tagctacaac acctaatact
     3301 tctgcacctg ctagttttaa ggcttcaact gctgtgactg aagatccccc tgtcgaaatt
     3361 aaatcttcta tcacaactac ttttttacct tcacttttag caccttcgat ttgattttgc
     3421 ttaccatgac tcttatttga tgaacgaaca taattcattg gtaattttaa tttttcagca
     3481 ataaaagctg catgtggaat accagctgtt gcagtaccag aaataacttc tacttcagga
     3541 aagtgttctt taattaagtt aattaaacca tcgcggattg cgcctcgaac taaaggataa
     3601 cctaacgtaa cacggttatc acagtaaatc ggtgatttaa tacctgaact ccatgtatat
     3661 aaatcatttg gtgataatgt tacagcttca atatctaata atgattttgc aatttcttta
     3721 gccattatac taaccaactt tctttaattt tatgataact ttcgactgga ttgtcacttt
     3781 gtgtaatcgg tctaccgact acaatatgcg tcgaaccaag ctgtcttgct tcttccggtg
     3841 tcgtaatacg gtgttggtca ttttgagatg cacctttagg tctaatacct ggtgttactt
     3901 ttaaaaatga tgtacctaac ttttcagtca acatacgact ttcaagaggt gaacaaacaa
     3961 cgccatctaa accagctgca tttgctaact tggcataatt taaaacggcc tcttcaatcg
     4021 atgtttgtat attttgttcg tgtcgtaaca tgtcttctgt tgttgacgta agctgtgtta
     4081 ctgcaataat ttttgtatct tgattatgtt ttcttaatcc tttaatggcc tcagacatca
     4141 tttttacgcc accagcagca tgaacattta ccagatcaac attcaattta gctagtcctt
     4201 ccatcgcctt accaactgta ttaggaatat catgcagttt taaatctaaa aatacatcat
     4261 ggcctctttc ttttatctca ttaattaatt gaggaccttc ttgataaaaa agttccatac
     4321 ctacttttac gaataatgat tcatcaaata aatctaaaaa ttgatttact ttttcttttg
     4381 attcaaaatc taatgcaa
//
LOCUS       DAKTIO010000192         4302 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.197, whole genome shotgun sequence.
ACCESSION   DAKTIO010000192 DAKTIO010000000
VERSION     DAKTIO010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.197"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            68..1027
                     /locus_tag="O0293_002445"
     CDS             68..1027
                     /locus_tag="O0293_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002484669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCY0281307.1"
                     /translation="MSKQFFTVEENYKERFYQLPKVFFTNPNYKDLSNDAKIAYAILR
                     DRLQLSIKNNWIDTEGNIYFIYTVADLEVILNCGNKKITKIKKELENVDLLIQKRQGL
                     NKPNLLYLLKPAITKNDIYEIDKAENEVEALQDKEVSKGHVQKCQKDTSRNVKRTRLE
                     MSKGHTNDTDFIDTDFIDTESNDMNNMNDTNQHSNHSNHFSNIHDKESLKYIELQELP
                     ELIKSYINNFSYEEVKSIKSVILKAKKSFNNKYDTFYMLEDIDEELLLVLKRFKGYLV
                     KKQEKVANMEGYLMRSIIAELEEMHSTIMRRKNMENNPLSLFN"
     gene            1305..1613
                     /locus_tag="O0293_002446"
                     /pseudo
     CDS             1305..1613
                     /locus_tag="O0293_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
     gene            1613..2359
                     /locus_tag="O0293_002447"
     CDS             1613..2359
                     /locus_tag="O0293_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0281308.1"
                     /translation="MNRLNTNYFDNFYIDWQNNEISENYVEFCQKQINNLSHIVPSLK
                     KEKRKIIDTCCGCGFHSKFLIDKGHHVISFDKSEEMLNFAYKKNGILGKRQSLDEEFP
                     ENHVDLILNLNTSIGYKKNDLKNLLRNSYTSLKKGGILIIDLMNPAFFYKNFEEQMIF
                     NFKDYEVNQTIKINKIYNSIVSTWDVYKNNEYSKDLSRELKFKLYTTNDIICSSLYYN
                     FTLSNISFYGDMKGNEFHENSPRLIVKITK"
     gene            2386..>4302
                     /locus_tag="O0293_002448"
     CDS             2386..>4302
                     /locus_tag="O0293_002448"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCY0281309.1"
                     /translation="MTKKYLNTQNEISAFWNTQKIFKKSIDNRKGQESFVFYDGPPTA
                     NGLPHAGHVLGRVIKDLVARLKTMQGFYVERKAGWDTHGLPVELEVEKKIGIKGKQDI
                     EKYGIENFINECKKSVFNYEKEWRDFSKDLGYWVDMDSPYITLENNYIESVWNILSTF
                     HKKGLLYKGHKVTPYCTHDQTALSSHEVAQGYKNVKDLSAVVKFQLTNSKDTYFLSWT
                     TTPWTLPANVALAINKDLNYSKIRVENEYYILATDLINSIITEKYEIIDTFSGSNLIN
                     LKYIPPFESDGLVNAYYVVDGEFVTNSEGTGIVHIAPAHGEDDYQLVLERDLDFLNVI
                     TREGVYNDRFPELVGNKAKNSDIEIIKLLSKKQLLYKKQKYEHNYPHCWRCGNPLIYY
                     AMEGWFIKTTNFKNEIINNNNNIEWFPSHIKEGRMGNFLENMVDWNIGRNRYWGTPLN
                     VWICNDCNHEYAPSSIKDLQNNSINKIDEDIELHRPYVDNITLSCPKCNGKMSRVEEV
                     IDVWFDSGSMPFAQHHYPFDNQKIFNQHFPADFIAEGVDQTRGWFYSLLVISTILKGK
                     SSYKRALSLGHILDSNGKKMSKSKGNVINPTELINKYGADSLRWALISDSAPWNNKRF
                     SENIVAQTKSKFIDT"
BASE COUNT         1711 a          525 c          667 g         1399 t
ORIGIN      
        1 tattcatcac ttgtaaataa cgatattact taaactcctt aaatatacat ttaaaggagt
       61 ttttatcatg tctaaacaat tttttacagt agaagaaaat tataaagaac gcttttacca
      121 attacctaaa gtatttttta ctaatcctaa ttataaagat ttatcgaatg acgctaaaat
      181 tgcttacgca atattgagag atcgtttaca attatctatt aaaaataact ggatagatac
      241 agaaggtaac atatatttca tttatacagt tgctgattta gaagtgatat taaattgtgg
      301 gaacaaaaaa atcactaaaa ttaaaaaaga attagaaaat gtagatttac taatccaaaa
      361 aaggcagggt ttaaacaaac caaatctact ttatttatta aaaccagcca taactaaaaa
      421 tgatatttat gaaatagata aggctgaaaa tgaagttgag gccttacaag acaaggaagt
      481 gtcaaaagga cacgtccaga aatgtcaaaa ggacacgtct agaaatgtca aaaggacacg
      541 tctagaaatg tcaaaaggac acacaaatga tactgatttt attgatactg attttattga
      601 tactgaaagt aatgatatga ataatatgaa tgatacaaat caacattcaa atcattcaaa
      661 tcacttttca aatattcatg ataaagaatc tcttaaatat atagaacttc aagaactccc
      721 cgaattaatt aaaagttata ttaataattt cagctatgaa gaagttaaaa gtattaaatc
      781 tgttatttta aaagctaaaa aatcatttaa taacaaatac gatactttct atatgttaga
      841 agatatagat gaagaattac tattggtttt aaaacgattt aaaggttatc ttgttaaaaa
      901 acaagaaaaa gtagctaata tggaaggtta tttaatgaga agtatcattg ctgaacttga
      961 agaaatgcac tcaactatta tgagaagaaa aaatatggaa aacaatccat tatctctatt
     1021 taattagttt ttgaagttaa attttcaaaa aaaagagatt atacacctaa aaaggtgtat
     1081 aatctttacg gttctgttgc aaagttgaat ttatagtata attttaacaa aaaggagtct
     1141 tctgtatgaa ctatttggtt ctgttgcaaa gttaaaaatc atgtaatatt tatactgaaa
     1201 aacgaaaaat aattaatact aacaaaactc tttactgtta aaacaatatt tgcttttata
     1261 gttttgtata gatgtgcata taattttaac tttgcaacag aaccgaaata actattccat
     1321 ttctaacaaa taatactgat agttctaatt atatttttgt tatatttgta aaaaatccta
     1381 ttttgaaaga aaaaatgatt actgatctta gaaatgagaa gatacagact agtttccatt
     1441 atactccctt acataaattt agttattata aatcaaaaaa cttattacca attgtagagg
     1501 aggtttctaa aggtttagtt acattaccct tacataatcg tttatctatc aatgatgtaa
     1561 aaagggtctg cactattatt agtaaaagta tacaggaatg gagagagcaa taatgaatcg
     1621 gctaaataca aattactttg ataactttta tattgattgg cagaacaatg aaatatcaga
     1681 aaattatgtt gaattttgtc aaaagcaaat taataactta agtcacattg ttccttcatt
     1741 aaaaaaagag aaaagaaaaa tcatcgatac ttgttgtggc tgtggttttc attctaaatt
     1801 tttgatagat aaaggtcatc atgttattag ctttgacaaa tctgaggaaa tgttgaactt
     1861 tgcatataaa aaaaatggca ttttaggtaa aagacaatct ttagatgaag aattcccaga
     1921 aaatcacgtc gacttaattc ttaatttaaa tacttctatc ggatataaaa aaaatgattt
     1981 aaaaaatttg ttaagaaata gttatacctc cttaaaaaaa ggtggtattt taattataga
     2041 tttgatgaac cccgcctttt tctataaaaa ttttgaagaa caaatgattt tcaacttcaa
     2101 agattacgaa gttaatcaaa ctattaaaat aaataaaatt tataatagta tagtttcaac
     2161 atgggatgtt tataaaaata acgaatatag caaagacctt tcaagagaat taaaatttaa
     2221 attatatact actaatgata taatttgcag tagtttgtat tataacttta ctttatccaa
     2281 tatatctttc tatggagaca tgaaaggaaa tgaatttcat gaaaattcac caagactaat
     2341 agtaaaaata acgaaataag tgatactcta ggaggctgaa aagttttgac aaagaaatat
     2401 ttaaacaccc agaatgaaat atcagcattt tggaatactc aaaagatatt taaaaaatca
     2461 attgacaata gaaaaggaca ggaaagtttt gttttttatg acggcccccc aactgcaaat
     2521 ggccttcctc atgctggcca tgttcttgga agagtaatca aggatttagt tgcaagatta
     2581 aaaactatgc aaggttttta tgtagaaaga aaagcaggat gggataccca tggcttacca
     2641 gttgaattag aggttgaaaa aaaaattgga attaaaggaa aacaagacat tgaaaagtat
     2701 ggaatagaaa attttataaa tgaatgtaaa aaaagtgtat ttaattatga aaaagaatgg
     2761 cgggattttt ctaaagattt aggatactgg gttgacatgg actcccccta tataactctt
     2821 gagaataatt atattgaaag tgtatggaat atattatcta cattccataa aaaaggacta
     2881 ttatataagg gacataaggt gactccttat tgtacacatg atcaaaccgc tttaagttct
     2941 catgaagtag cgcaaggcta taaaaacgtt aaagatttat cagctgttgt taaatttcaa
     3001 cttacaaata gtaaagatac ttatttctta agttggacta ccactccctg gactttgcct
     3061 gcaaatgtag cattagctat aaataaagat cttaattatt caaaaattcg ggtagaaaat
     3121 gagtattata tcttagctac agatctaatt aattctataa taactgaaaa atacgaaatt
     3181 attgatacct tttcaggaag taatttaatt aatttaaaat acattcctcc ttttgaaagc
     3241 gacggtttag ttaatgcata ttacgttgtt gatggagaat ttgttactaa ctcagaagga
     3301 actggtattg ttcatatagc accagctcat ggggaagatg actaccaatt ggttttagag
     3361 cgtgatttgg atttcttaaa tgttataaca agagaaggag tatataatga taggttccct
     3421 gaattagttg gtaataaagc taaaaatagt gatatagaaa tcataaaatt attatccaaa
     3481 aaacaacttt tatataaaaa acaaaaatat gagcataatt atcctcattg ttggagatgt
     3541 ggtaatcctt tgatatatta tgcgatggaa ggttggttta ttaaaacaac taattttaag
     3601 aatgaaatta ttaacaataa taataatata gagtggtttc cttctcatat taaggaaggg
     3661 agaatgggaa atttcttaga aaatatggtt gattggaaca ttggtagaaa tagatattgg
     3721 ggaacaccat taaatgtatg gatttgcaat gattgtaatc acgaatacgc accaagtagt
     3781 attaaggatt tacaaaataa ttccatcaat aaaattgatg aagatattga gttgcataga
     3841 ccttatgttg ataatatcac tcttagttgc cctaagtgta atgggaaaat gtctcgagta
     3901 gaagaagtaa tcgatgtttg gtttgatagc ggctctatgc cgtttgctca gcatcattat
     3961 ccttttgata accagaaaat ttttaatcaa cactttccag ctgattttat tgcagaagga
     4021 gttgatcaaa cgagaggctg gttttacagt ttactagtaa tttctactat tctaaaagga
     4081 aaatcttctt ataaacgtgc tttatcttta ggacatattc tagacagtaa tggtaaaaaa
     4141 atgtctaaaa gtaaaggaaa cgttattaat ccaactgaat taattaataa gtacggagcc
     4201 gattctttaa gatgggcctt aatttcggat agtgctccat ggaataacaa aagattctca
     4261 gaaaatatag tagctcagac caaatcgaaa tttatagata cg
//
LOCUS       DAKTIO010000193         4229 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.198, whole genome shotgun sequence.
ACCESSION   DAKTIO010000193 DAKTIO010000000
VERSION     DAKTIO010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.198"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..1849)
                     /locus_tag="O0293_002449"
     CDS             complement(<1..1849)
                     /locus_tag="O0293_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation protein NorD"
                     /protein_id="HCY0281310.1"
                     /translation="MSDRFIKFNDEQLDAKQVMMLQDLARLLLKNEQTQVKIQKFPYY
                     NPVQNVLITSWFWSHRPSHIEMAGLKTDVMLAAYGYHMMDVQIVNEVVQDKTFKHPKF
                     YQQLFKLLEDMRVLNSIKVERPSTAKLIDLRLDTRISYTESQIKVYRTKTQYTDLLFL
                     YLEHAFLSQDFFDIPSIHSDLDDILVNMFLYLPNFFQNQNSEDNMYLAQRIMYQVDDI
                     LKEDMLNEYYYLPKTLYNTLASPEFDDLKRTDASQVDGQDDTSEDDDNESEKADSKSA
                     DSESKGGAYLEMELHEGQNSETLGNDEAREGDATDDMTDMMTKKGKGSNDTLNREEGD
                     AVGQSQAFQLDGVNKNVEIKWQIPEIEPQYVLEYQESKQDVQYEIKDLIQIIKKTIER
                     EQRDARFNLTKGRLQKDLINWFIDDQYKLFYKKQDLSKSFDATFTLLIDASASMHDKM
                     AETKKGVVLFHETLKALNIKHEILSFSEDAFDSDEHAQPNIINEIINYDYSTFEKDGP
                     RIMALEPQDDNRDGVAIRVASERLMRRNQHQRFLIVFSDGEPSAFNYSQDGIIDTYEA
                     VEMSRKFGIEVFNVFLSQDPITEDVEQTIHNIYGQYAIFVEGVAHLPGHL"
     gene            complement(1863..2654)
                     /locus_tag="O0293_002450"
     CDS             complement(1863..2654)
                     /locus_tag="O0293_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCY0281311.1"
                     /translation="MALKHYKNSDSTVFNDAKALFDLNKNILLKGPTGSGKTKLAETL
                     SEVVDTPMHQVNCSVDLDTESLLGFKTIKTNAEGQQEIVFVDGPVIKAMKEGHILYID
                     EINMAKPETLPVLNGVLDYRRQITNPYTGEVIKAVPGFNVIAAINEGYVGTLPMNEAL
                     KNRFVVIHVDYIDGDILKNVIKEQSLLQGDKQIEQIIKFNEDLRTMSKQGQISEEAAS
                     IRALLDLCDLITVMPVERAIKRTIIDKLEDEREQQAIYNAVELNF"
     gene            complement(2835..3038)
                     /locus_tag="O0293_002451"
     CDS             complement(2835..3038)
                     /locus_tag="O0293_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281312.1"
                     /translation="MLHETWKERTPIKKVEVINTDAKKFTVSDMLTVGKQYDVINETE
                     EYYQIIDNSGLVGGYYKTYFKEV"
     gene            complement(3067..3876)
                     /locus_tag="O0293_002452"
     CDS             complement(3067..3876)
                     /locus_tag="O0293_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0281313.1"
                     /translation="MCGLRSITLGTTNIEQTKHFMVDILGLNYEELLENSIRFGDADI
                     SPGTRLQFIQVPSEQLEESHFVGIGLRTPTDSGLEEYAEILSNKDIPFTTVKELNGNK
                     YFSLEDNNGHIFSIYSNENNYGVGLGMPSFESAVNPLHQVQGLGPVILKVNHVDITGQ
                     ILTNIFGLEVFAEYQPFDNADYHVQVFKVGTGGLGGEIHLMPVETEMTMPEYGAVDQV
                     EFETKDADFFNQAKSRLDEVEIPYQTLEQDDIESIRITENSGLSFIFNLQK"
BASE COUNT         1303 a          748 c          580 g         1598 t
ORIGIN      
        1 agagatgtcc aggtaaatgc gcaacacctt caacgaaaat agcatattga ccataaatat
       61 tatgaattgt ttgttcaaca tcttcagtta ttggatcttg actcaaaaat acattaaata
      121 cttcaatacc aaatttacgt gacatttcta cagcttcata cgtatcaata ataccatctt
      181 gactataatt aaatgcagac ggttcgccgt ctgaaaatac gattaaaaat cgttgatgtt
      241 gatttcgacg cattaatcgt tcacttgcaa ctctaatagc aacaccatca cgattatcat
      301 cttgaggttc aagtgccata atacgtgggc catctttttc aaaggttgag taatcatagt
      361 taataatttc attaatgata tttggttgtg catgctcgtc tgaatcaaag gcatcctcac
      421 tgaatgataa aatttcatgt ttaatgttca atgcttttaa cgtctcgtgg aataacacta
      481 cacctttctt cgtttcagcc attttatcat gcatacttgc tgacgcatca attaacaatg
      541 taaatgtcgc atcaaatgat ttacttaaat cttgtttttt gtaaaacaat ttatattgat
      601 cgtcgataaa ccaattaatt aaatcctttt gtaatcgccc ttttgttaaa ttaaaacgtg
      661 catctcgttg ttctcgttca atcgttttct ttataatttg aattaagtct ttgatttcat
      721 attgtacgtc ttgtttcgat tcttgatatt ctaaaacata ttgtggctca atttcaggaa
      781 tttgccattt tatttctaca tttttgttca caccatcaag ttgaaatgct tggctttgac
      841 caacagcatc gccttcttca cgatttagcg tatcgtttga tcctttgccc tttttggtca
      901 tcatatcagt catatcatct gtagcatcgc cttcacgtgc ttcatcatta cctaatgttt
      961 cactattttg tccttcgtga agctccattt ctaaataggc gccacctttt gactcactat
     1021 ctgcagattt agagtcagct ttttcacttt cgttatcatc atcttctgaa gtatcatctt
     1081 gcccatcgac ttgacttgca tcagttctct ttaaatcatc aaactctgga ctagctaatg
     1141 tattatatag tgttttaggt aagtaataat attcgtttag catatcttct tttaaaatat
     1201 catctacttg atacataatt ctttgagcca gatacatatt atcttcagaa ttttgatttt
     1261 ggaaaaaatt aggtaaatat aagaacatat ttactaaaat atcatctaaa tctgaatgta
     1321 tagatggtat atcaaagaaa tcttggctta aaaatgcatg ttctaaatac aaaaataata
     1381 aatctgtata ttgtgtttta gtacgataca ctttaatttg agattccgta tatgatatac
     1441 gtgtatctaa gcgaagatca attaatttag cagtacttgg gcgctcaact ttaatagaat
     1501 ttaatacgcg catatcttct aataatttaa aaagttgttg ataaaattta gggtgtttaa
     1561 aagttttatc ttgtactact tcatttacaa tttgtacatc catcatatga taaccgtaag
     1621 cagctaacat aacatctgtt tttaaaccag ccatttcgat atggcttggt cgatgtgacc
     1681 aaaaccaact cgtaatcaaa acattttgaa caggattata gtaagggaat ttttggattt
     1741 taacttgtgt ttgttcattt tttaataaga gtcgagctaa gtcttgcaac atcattactt
     1801 gcttggcatc taactgttcg tcattaaatt ttatgaaacg atcactcatg ttttatccct
     1861 ctttaaaagt ttagttctac agcattatat attgcttgtt gttcacgttc atcttccaat
     1921 ttatcaataa ttgtacgttt aattgcacgt tcaactggca ttacagtgat taaatcacac
     1981 aagtctaata atgcacggat actagcggct tcttcagaaa tttgtccctg cttagacata
     2041 gtacgtaaat cttcattaaa cttaataatt tgttcgattt gtttatcacc ttgtaataaa
     2101 ctttgctctt taatcacatt ttttaaaatg tccccatcaa tataatcaac gtgaataaca
     2161 acaaagcgat tttttagtgc ttcattcatt ggcaaagtac caacataacc ttcatttatc
     2221 gctgctataa cgttaaatcc tggtacagct ttgattactt caccagtgta tggattcgta
     2281 atttgacgac gataatctaa gaccccattt aatacaggca atgtttcagg tttagccata
     2341 tttatttcat caatatataa aatatgcccc tctttcatag ctttaataac tggaccatct
     2401 acaaagacaa tttcttgttg accttccgca tttgttttaa ttgttttaaa gcctaataag
     2461 ctttctgtat ctaaatcaac agaacaattg acttgatgca tgggtgtatc aacaacttca
     2521 cttaatgttt ctgccaactt tgttttccct gaacctgttg gacctttaag taaaatattt
     2581 ttatttaaat caaataatgc cttcgcatca ttgaaaactg ttgaatctga attcttataa
     2641 tgttttagtg ccattttaaa acgttctcct ttaaataaat taaaaaacta agacatggaa
     2701 taagttctcc aaatgcctta gtttactatt ctattctgat ataaaacgca atgcttagta
     2761 tgcgaattgt ttatatttta ccaaatttac taattgaaaa tctcatatta ggcttcattt
     2821 caataattat gcgtttaaac ttctttgaaa tatgttttat agtaaccgcc aactaatcca
     2881 gaattatcta ttatttgata atattcttca gtttcgttga taacatcata ttgtttgcca
     2941 accgttaaca tgtcagaaac tgtaaatttc tttgcatctg tattaatgac ttctactttc
     3001 ttgattggtg tacgttcttt ccaagtttca tgtaacataa attttgtcct cgctatcgta
     3061 aaaaaattat ttttgtaaat tgaatataaa cgataatcca ctgttttcag taattctaat
     3121 tgattcaata tcatcttgct caagcgtttg atatggtatt tccacttcat ctaagcgcga
     3181 tttcgcttga ttaaagaaat ctgcatcttt cgtttcaaac tcaacttgat cgactgcgcc
     3241 atattccggc attgtcattt cggtttctac aggcattaaa tgtatttcgc cacctagacc
     3301 acccgttcca actttgaata cttggacatg atagtcagca ttgtcgaagg gttggtattc
     3361 tgcaaatact tcaagtccga atatatttgt taaaatttga cctgtaatat ctacatgatt
     3421 cactttaaga atcactggtc ctaaaccttg cacttgatgt aacggattga ccgcactctc
     3481 aaaagaaggc atacctaaac caacgccata attattctcg tttgaatata ttgagaaaat
     3541 atgaccattg ttatcttcga gactgaaata tttattgcca tttaattctt taactgttgt
     3601 aaatggaata tccttattcg ataatatttc cgcatactcc tctaaacctg agtcagttgg
     3661 tgtacgtaat ccaataccca caaagtgaga ttcttctaat tgctcacttg gaacttgtat
     3721 aaattgaagt cttgttcctg ggcttatatc tgcatcgccg aaacgaattg agttttcaag
     3781 aagttcttca taatttaatc ctaatatgtc aaccatgaaa tgttttgtct gttctatatt
     3841 tgttgtacct aatgttatac ttctaagtcc acacattata gccacctcta tctttatctt
     3901 tactattata ttataactta ttttaatcat aaattataga atatcggtca gttgattttg
     3961 attatagatt aatatgtgaa aaatgtattt ctttaaaaga actatttcat ttaaataggt
     4021 aacataatat tgttatggtt gctattgagt gtgtcattaa tgaatatctg tatatagcaa
     4081 ttgaatattt cgttttttgt aggacatgaa aaacatatat atgtcgtgtc cataaagcga
     4141 atttacatca tgttcagatg attttaaatt aactaaaggc gcttatagaa ttcatatatt
     4201 tcatcgtttg ttattatcta aaatgcatt
//
LOCUS       DAKTIO010000194         4222 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.199, whole genome shotgun sequence.
ACCESSION   DAKTIO010000194 DAKTIO010000000
VERSION     DAKTIO010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.199"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(336..1559)
                     /locus_tag="O0293_002453"
     CDS             complement(336..1559)
                     /locus_tag="O0293_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HCY0281314.1"
                     /translation="MTTFSEKEKIQLLADIVELQTENNNEIDVCNYLTDLFDKYDIKS
                     EILKVNEHRANIVAEIGNGSPILALSGHMDVVDAGNQDNWSYPPFQLTEKDGKLYGRG
                     TTDMKGGLMALVVSLIELKEQNELPHGTIRLLATAGEEKEQEGAKLLADKGYLDDVDS
                     LIIAEPTGSGIYYAHKGSMSCKVTATGKAVHSSVPFIGDNAIDTLLEFYNLFKEKYSE
                     LKQQDTKHELDVAPMFKSLIGKEISEEDANYASGLTAVCSIINGGKQFNSVPDEASLE
                     FNVRPVPEYDNDFIESFFQNIINDVDSNKLSLDIPSNHRPVTSDKNSKLITTIKDVAS
                     SYVEQDEIFVSALVGATDASSFLGDNKDNVDLAIFGPGNPLMAHQIDEYIEKDMYLKY
                     IDIFKEASIQYLKEK"
     gene            1994..3049
                     /gene="lukH"
                     /locus_tag="O0293_002454"
     CDS             1994..3049
                     /gene="lukH"
                     /locus_tag="O0293_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit H"
                     /protein_id="HCY0281315.1"
                     /translation="MKNKKRVLIASSLSCAILLLSAATTQANSAHKDSQDQNKKEHVD
                     KSQQKDKRNVTNKDKNSTAPDDIGKNGKITKRTETVYDEKTNILQNLQFDFIDDPTYD
                     KNVLLVKKQGSIHSNLKFESHKEEKNSNWLKYPSEYHVDFQVKRNRKTEILDQLPKNK
                     ISTAKVDSTFSYSSGGKFDSTKGIGRTSSNSYSKTISYNQQNYDTIASGKNNNWHVHW
                     SVIANDLKYGGEVKNRNDELLFYRNTRIATVENPELSFASKYRYPALVRSGFNPEFLT
                     YLSNEKSNEKTQFEVTYTRNQDILKNRPGIHYAPPILEKNKDGQRLIVTYEVDWKNKT
                     VKVVDKYSDDNKPYKEG"
     gene            3071..4087
                     /gene="lukG"
                     /locus_tag="O0293_002455"
     CDS             3071..4087
                     /gene="lukG"
                     /locus_tag="O0293_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit G"
                     /protein_id="HCY0281316.1"
                     /translation="MIKQLCKNITICTLALSTTFTVLPATSFAKINSEIKQVSEKNLD
                     GDTKMYTRTATTSDSQKNITQSLQFNFLTEPNYDKETVFIKAKGTIGSGLRILDPNGY
                     WNSTLRWPGSYSVSIQNVDDNNNTNVTDFAPKNQDESREVKYTYGYKTGGDFSINRGG
                     LTGNITKESNYSETISYQQPSYRTLLDQSTSHKGVGWKVEAHLINNMGHDHTRQLTND
                     SDNRTKSEIFSLTRNGNLWAKDNFTPKDKMPVTVSEGFNPEFLAVMSHDKKDKGKSQF
                     VVHYKRSMDEFKIDWNRHGFWGYWSGENHVDKKEEKLSALYEVDWKTHNVKFVKVLND
                     NEKK"
BASE COUNT         1565 a          619 c          659 g         1379 t
ORIGIN      
        1 ttatttgata gaaatcagct tttttcaaat gtatttgata atttacagct cgttgagctg
       61 ctgttttcta gggatttatg tcccattcct tttacgagaa ggtttttacg agttttatgt
      121 aactattaga agctatcttt ttatatgacc attagtcaat atatgtatga caaatcagca
      181 caataatttc agttttacat cacttaatca atgaaaatgt gcttgttgat atttgaatat
      241 ttacattcaa acaatattca tcgctatttc tatcaactta aaattcgcaa taaaaaactg
      301 ccagcacgcg aatagggcag ctgacagcat cgttcttatt tttcttttaa atattgaatg
      361 gaagcctctt taaaaatatc aatatatttc agatacatat ctttttcaat atattcatcg
      421 atttgatgtg ccattaatgg attacctggt ccaaaaatgg ctaaatcaac attgtcctta
      481 ttatctccta agaaactaga ggcatctgtt gcacctacaa gcgctgaaac aaatatttcg
      541 tcttgttcta cataactaga agctacatct ttaatcgtag taattaattt gctattttta
      601 tcgcttgtta caggtcggtg attgcttgga atatcgagtg aaagcttatt gctatccaca
      661 tcattaatga tattttggaa aaacgattct ataaagtcgt tatcatactc aggaactggt
      721 cttacgttaa attcaagtga agcttcatct ggtacagagt taaattgttt gccgccattt
      781 ataatcgaac atacagctgt aagaccagat gcataatttg catcctcttc agaaatttct
      841 tttccaatca atgatttgaa cataggcgca acatctaatt catgtttagt atcttgttgt
      901 ttaagctctg aatatttttc tttaaataga ttataaaatt caagcagtgt atcaattgca
      961 ttgtcaccaa taaatggaac tgagctatgg acagctttac cagttgcagt tactttacat
     1021 gacatagacc ccttatgtgc ataataaatt ccagatccag ttggttcagc aataattaag
     1081 ctatcgacat cgtctaaata gcctttatca gctaataatt tggcaccttc ttgttctttc
     1141 tcttcgccag cagtagccag taatctaatc gttccatgag gcaattcatt ttgttctttt
     1201 aattcgatta gagatacgac caaagccatt aaaccgcctt tcatatctgt agtgcctcgg
     1261 ccatataatt tgccatcttt ttctgtcagt tgaaaagggg gatatgacca attatcttga
     1321 tttcctgcat caacaacatc catatgacca ctcaatgcga gtataggtga gccgttaccg
     1381 atttctgcaa cgatattggc gcggtgttca ttaactttca aaatttcaga tttaatatcg
     1441 tacttgtcga ataaatctgt taaataatta caaacgtcta tttcattatt attttcagtt
     1501 tgtagttcaa caatatctgc tagtaattga attttttctt tttcactaaa agttgtcatt
     1561 aagctcacac ctttcaaagt agtatatata tttatataaa cataattttt attcttaaac
     1621 ataatttaaa atgcagaaag tagtgtgtta tttgatttcg ttctatgaaa ttaactgtct
     1681 agtatcaacg atcttattaa cgctgatatc ggatatgctt aattttatag taaattgtat
     1741 gagaaagatg caggatatta tttagctaat aattatatat aaatttcaaa tcgatggtta
     1801 ttaactacta aataaaaata tttgctaaat taatgacttt gtacacacat tatttttaaa
     1861 gaggataaag tatttaataa tattaacaaa atcatttaat aaatagttaa atatatattc
     1921 tctgtttttg tgatattatt cacatgtcga tacatatcaa caaatatcaa tcatacgaaa
     1981 gaaggttata acaatgaaaa ataaaaaacg tgttttaata gcgtcatcat tatcatgtgc
     2041 aattttattg ttatcagcag caacgactca agcaaattca gctcataaag actctcaaga
     2101 ccaaaataag aaagaacatg ttgataagtc tcaacaaaaa gacaaacgta atgttactaa
     2161 taaagataaa aattcaacag caccggatga tattgggaaa aacggtaaaa tcacaaaacg
     2221 aactgaaaca gtatatgatg agaaaacaaa tatactccaa aatttacaat tcgactttat
     2281 cgatgatcca acttatgaca agaatgtatt acttgttaaa aaacaaggct caattcattc
     2341 aaatttaaag tttgaatctc ataaagaaga aaaaaattca aattggttaa agtatccaag
     2401 tgagtaccat gtagattttc aagtaaaaag aaatcgtaaa actgaaatat tagaccaatt
     2461 gccgaaaaat aaaatttcaa ctgcaaaagt agacagtaca ttttcatata gctcaggtgg
     2521 taaattcgat tcaacaaaag gtattggacg aacttcatca aatagctact ccaaaacgat
     2581 tagttataat cagcaaaatt atgacacaat tgccagcggt aaaaataata actggcatgt
     2641 acactggtca gttattgcga atgacttgaa gtatggtgga gaagtgaaaa atagaaatga
     2701 tgaattatta ttctatagaa atacgagaat tgctactgta gaaaaccctg aactaagctt
     2761 tgcttcaaaa tatagatacc cagcattagt aagaagtggc tttaatccag aatttttaac
     2821 ttatttatct aatgaaaagt caaatgagaa aacgcaattt gaagtaacat acacacgaaa
     2881 tcaagatatt ttgaaaaaca gacctggaat acattatgca cctccaattt tagaaaaaaa
     2941 taaagatggt caaagattaa ttgtcactta tgaagttgat tggaaaaata aaacagttaa
     3001 agtcgttgat aaatattctg atgacaataa accttataaa gaaggataat attgaaaggg
     3061 cggattacta atgattaaac aactatgtaa aaatatcaca atttgtacgt tagcactatc
     3121 gactactttc actgtattac cagctacttc atttgcaaag attaattctg aaatcaaaca
     3181 agtttctgag aagaatcttg atggtgatac taaaatgtat acacgtacag ctacaacaag
     3241 tgatagtcaa aaaaatatta ctcaaagctt acaatttaat ttcttaactg aacctaatta
     3301 tgataaagaa acagtattta ttaaagcaaa aggtacaatt ggtagtggtt tgagaatttt
     3361 agacccaaat ggttattgga atagtacatt aagatggcct ggatcttatt cagtttcaat
     3421 tcaaaatgtt gatgacaaca acaatacaaa tgtgactgac tttgcaccaa aaaatcagga
     3481 tgaatcaaga gaagttaaat atacgtatgg ttataaaaca ggtggagatt tttcgattaa
     3541 tcgtggaggc ttaactggaa atattacaaa agagagtaat tattcagaga cgattagtta
     3601 tcaacaacca tcatatcgta cattacttga tcaatctacg tcacataaag gtgtaggttg
     3661 gaaagtagaa gcacatttga taaataatat gggacatgac catacgagac aattaactaa
     3721 tgatagtgat aatagaacta aaagtgaaat tttttcttta acacgaaatg gaaatttatg
     3781 ggcgaaagat aatttcacac ctaaagacaa aatgcctgta actgtgtctg aagggtttaa
     3841 tccagaattt ttagctgtta tgtcacatga taaaaaagac aaaggtaaat cacaatttgt
     3901 tgttcattat aaaagatcaa tggatgagtt taaaatagat tggaatcgcc atggtttctg
     3961 gggctattgg tctggtgaaa accatgtaga taaaaaagaa gaaaaattat cagcattata
     4021 tgaagttgat tggaagacac ataatgtgaa gtttgtaaaa gtacttaatg ataatgaaaa
     4081 gaaataagta ataaaagttg cctgctacat agaatgtagt aggtaacttt tatttatatt
     4141 tgaatagata gatttattat gacgtgcagt gtatgaatct tgtttgagtg tagagtaaag
     4201 acttgtatta atgaaaaatt aa
//
LOCUS       DAKTIO010000195         4146 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.200, whole genome shotgun sequence.
ACCESSION   DAKTIO010000195 DAKTIO010000000
VERSION     DAKTIO010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.200"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            173..1099
                     /locus_tag="O0293_002456"
     CDS             173..1099
                     /locus_tag="O0293_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0281317.1"
                     /translation="MNIVGHHHISMYTKDAKRNKDFYTNVLGLRLVEKSVNQDNPSMY
                     HLFYGDEVGTAGTILSFFEIPNAGHKQPGTETIYRFSLLVPNQAALHYFEKRLENNGI
                     KSERLYYLGQEGVVFKDEDDLEIILLVNDSFEVPHQWQHNAYSEIPQAYQILGIGPVE
                     LRVRNAARTVEFLENVLGYRKRDNKSFDVLTLAPQGLYSDFVVIEQQGQRERPGRGYI
                     HHIAVNTPQMSDLDAIYKKLQQQPQSNSGIIDRYFFKSLYYRHNSIMYEFATEAPGFT
                     IDTPVEQLGSQLNLPDFLEAEREQIESKLHEI"
     gene            1113..2174
                     /locus_tag="O0293_002457"
     CDS             1113..2174
                     /locus_tag="O0293_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCY0281318.1"
                     /translation="MAKLEMNKNTPLEFGLYSLGDHLLNPLKGEKVSYEQRINEIIEA
                     SKLADEAGIDVFAVGESHQEHFTTQAHTVVLGAIAQATKHIKVSSSSTIISATDPVRV
                     FEDFATLDLISHGRAEIVAGRASRTGIFDLFGYDLKDYDELFEEKLGLLLELNKTERI
                     TWSGKYRPELRNMKIFPRPIDNILPIWRAVGGPPASAIKAGKQGVPMMITTLGGPAMN
                     FKGSIDAYRQAATEAGFDASPKSLPVSTASLFYTAETTQDAMREFYPHLNTGMSFIRG
                     VGYPKQQFANSSDYREALMVGSPQQIIEKILYQHELYGHQRFMAQLDFGGVPFENVMK
                     NIELIGNDIIPAIKKHLSK"
     gene            2188..2754
                     /locus_tag="O0293_002458"
     CDS             2188..2754
                     /locus_tag="O0293_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCY0281319.1"
                     /translation="MNIVLLSGSTVGSKTRIAMDDLKNELEVINEGHQIELMDLRELE
                     LEFSVGKNYLDTTGDVYKLTTSLMQADVIFIGFPIFQASIPGALKNVFDLLPVNAFRD
                     KVIGLVATAGSSKHYLIPEMHLKPILSYMKAHTMQTYVFIEEKDFSNQQIVNDDVVFR
                     LKALAQSTMRTAKVQQQVFEEENNQYDF"
     gene            complement(2814..3809)
                     /locus_tag="O0293_002459"
     CDS             complement(2814..3809)
                     /locus_tag="O0293_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCY0281320.1"
                     /translation="MASLKNKHFMIGLSLTFIVALFSFLAAKLPILDKVGALTIAILI
                     AILYRHFRGYPEQYSSGITFSSKYLLRFAIILYGLKLNIFDIIGQGSKLLAIDVGVVI
                     FSIVMMLFVNKLLHGDKNIALLLGVGTGVCGAAAIAAVAPIFKSREKDTAISIGIIAL
                     IGTIFSLIYTAIYAIFSMTTNVYGAWSGVSLHEIAHVVLAGGFGGSDALKIALLGKLG
                     RVFLLIPLTIVLILIMRFRSSESSSKGRISIPYFLIGFVIMALVNTYVTIPSALLNIL
                     NTVSTICLLMAMVALGLNVAFKDLKNRALKPLMTIIITSICLSSLAFIVVHWLYS"
BASE COUNT         1441 a          650 c          819 g         1236 t
ORIGIN      
        1 aaataaattg ctacacataa atttataatt atgcaattat tatgtcgtta tacatactag
       61 ctgtgtatct acgaatgagt acaaacatat ttttatttgc aaggggtaaa tggcatataa
      121 ctatcttttt tatgtaagct ggtataaaat ttatactaat aggagggata gtatgaatat
      181 agtagggcat catcacatat ccatgtatac aaaagatgca aaacgtaata aggattttta
      241 cacaaatgtc cttggattac gattagttga aaagtcggtt aatcaagaca atccttcaat
      301 gtatcatttg ttttatgggg acgaagtagg tacagccgga acaattttaa gcttttttga
      361 aattcccaat gcgggtcata agcagccagg tactgaaacg atttatcgat tttcattatt
      421 agtaccaaat caagcggcac ttcattattt tgaaaaacgt cttgagaata atggtattaa
      481 gtctgaacgt ttgtactatc ttggacaaga aggtgttgtc tttaaagatg aagacgactt
      541 agaaatcata ttgcttgtta atgatagttt tgaagtacca catcaatggc aacataacgc
      601 ttatagtgaa atacctcaag catatcaaat tttaggaata gggccagtcg aattaagagt
      661 tagaaatgca gcgcgtacgg tagaattttt ggaaaatgtc ttaggttatc gcaaaagaga
      721 taataaatca ttcgatgtgc tgacattagc accacaaggt ttatattcgg attttgtagt
      781 tattgagcaa cagggacaac gtgaaagacc tggacgaggt tatatccatc atattgcagt
      841 taatacacca caaatgagtg acttagatgc aatttacaag aaattacaac aacaaccaca
      901 aagtaattca ggtataattg atcgctattt ctttaaatca ttatactatc gccataattc
      961 aattatgtat gaatttgcga ctgaagcgcc tggatttact attgatacac ctgttgaaca
     1021 attaggaagt caattgaact tgcctgactt tttagaagca gaacgtgaac aaattgaaag
     1081 taagttacac gaaatataaa ggagaatgtt taatggccaa attagaaatg aataaaaata
     1141 cgcctcttga gtttggtttg tattccttag gtgatcattt attgaatcca ttgaaaggtg
     1201 aaaaagttag ttatgagcaa cgtattaatg aaattattga agcaagtaaa ttagcagatg
     1261 aagcaggtat tgatgttttt gcagttggtg aaagtcatca ggagcatttt acaacacagg
     1321 cacatacggt tgtgttaggt gcaattgccc aagcgacaaa gcatattaaa gtttcaagtt
     1381 cttcaacgat tattagtgca acagatcctg taagagtatt tgaagacttc gcgacattag
     1441 atttgatttc tcatggtaga gccgaaattg tagctggcag agcatcaaga acaggtattt
     1501 ttgacttgtt tggctatgat ttaaaagact atgatgaatt gtttgaagaa aaattaggtt
     1561 tacttttaga gttaaataaa actgagcgta ttacttggtc tggaaaatat cgtccagaac
     1621 ttagaaatat gaaaatattc ccaagaccaa tcgataatat attgccaata tggcgtgctg
     1681 ttggtggtcc acctgcaagt gctattaaag cgggaaaaca aggtgtgcca atgatgatta
     1741 caacccttgg tggcccagca atgaacttta aaggttctat agatgcttat cgtcaagcgg
     1801 caactgaagc aggtttcgat gcttcgccta agtctttacc agtaagtaca gcgagtctgt
     1861 tttatacagc tgaaacaact caggatgcta tgagagaatt ttatccacat ttgaatacag
     1921 ggatgtcatt tattcgtggt gttggttatc cgaaacagca atttgctaat tcgtcagatt
     1981 atcgagaagc gctaatggtt ggaagcccgc aacaaattat tgaaaagata ttgtatcaac
     2041 acgagttgta tggtcatcaa cgttttatgg cacagcttga ttttggcggt gtgccatttg
     2101 aaaatgttat gaagaatatt gagttaattg gcaacgacat tataccggcg attaaaaagc
     2161 atttatcaaa ataggagggg cgtcatcatg aatattgtat tattgtcagg ttccacagta
     2221 ggttctaaaa cgagaattgc tatggatgat ttaaaaaatg aactagaagt catcaatgag
     2281 ggacatcaaa tagagttgat ggatttacga gaacttgaat tagaatttag cgttggaaag
     2341 aattatctag atactacagg agatgtatat aaattaacga cgtcgttaat gcaggctgat
     2401 gtgattttta ttggttttcc aatttttcaa gcttccatcc ctggtgcttt gaaaaatgtg
     2461 tttgatctac ttccagtcaa tgcgtttcgt gacaaggtaa taggacttgt agcgacagca
     2521 ggttctagta aacattattt aattcctgaa atgcatttaa aaccaatatt gagttacatg
     2581 aaagcacata cgatgcaaac gtatgtattt attgaagaga aagatttttc aaatcaacaa
     2641 attgtcaatg atgatgttgt atttcggtta aaagcgttgg cacaatccac aatgcgaact
     2701 gccaaagtac aacaacaagt gtttgaagaa gaaaacaacc aatacgactt ttaaagtata
     2761 aaaataagac gctcggcaca ctaaatttgt aagtgtttga gcgtcttttc atattaacta
     2821 tatagccaat gaacgacgat aaaggcaagt gatgacaagc atattgaggt aataatgatt
     2881 gtcataagcg gtttaagtgc gcgattttta agatctttaa atgcaacatt taaccctaaa
     2941 gcaaccatgg ccattaataa gcaaattgtt gatacagtat ttaaaatatt tagcaatgct
     3001 gacggaatag ttacatatgt attcactaag gccataatga caaatccaat taaaaagtat
     3061 ggaatgctta ttcgaccctt gctagatgat tctgatgaac ggaaacgcat aattaaaata
     3121 agtacgatgg ttaatggaat cagtaagaat actctaccaa gtttaccaag aagtgcaatt
     3181 ttaagtgcat cactaccacc aaagccacca gctaagacaa cgtgtgcaat ttcatgaaga
     3241 ctaacaccag accaagcgcc ataaacattt gtcgtcattg aaaagatagc gtagatagct
     3301 gtatatataa gtgaaaatat cgtaccaatc aatgcgatga taccgatact aatagctgta
     3361 tccttttcac gtgatttgaa tattggagcg actgcggcaa tagcagcagc accacaaacg
     3421 cctgtgccga cacctagtaa taatgcgatg tttttgtcac catgcaacag tttgttgaca
     3481 aagagcatca ttacaatact gaaaataacg acacctacat cgatggctaa tagtttacta
     3541 ccttgaccga taatatcgaa tatattgagt ttaagtccat ataggatgat tgcaaatctt
     3601 aataaatatt tagatgaaaa cgtaatacct gagctatatt gttcaggata tcctctaaag
     3661 tgacgatata gaatagcgat taatatcgcg atagttaatg cgccaacctt atctaggatt
     3721 ggcaatttag ctgctaaaaa gctaaataat gcgactataa atgttagtga tagcccaatc
     3781 ataaaatgct tatttttcaa tgatgccatg agcagtgcct cctttaatag cattttagca
     3841 ctgttttgtc gtatttttaa atataaattt ggaatgaata ataaagtagt gattaaatta
     3901 agttgtgtga taggaaactt ggacatcaat caaagtaata ggcactacaa cgcttattgg
     3961 cggggcccca acaaagaagc tgacgaaaag tcagcttaca ataatgtgca agttggggat
     4021 gggccccaac atagagaaat tgggtccgta atttctacag acaatgcaag ttggcggggc
     4081 cccaacatag agaatttcga aaagaaattc tacaagcaat gcaagttggc ggggccccaa
     4141 cataga
//
LOCUS       DAKTIO010000196         4137 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.201, whole genome shotgun sequence.
ACCESSION   DAKTIO010000196 DAKTIO010000000
VERSION     DAKTIO010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.201"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(429..2237)
                     /gene="pepF"
                     /locus_tag="O0293_002460"
     CDS             complement(429..2237)
                     /gene="pepF"
                     /locus_tag="O0293_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCY0281321.1"
                     /translation="MSQQLSREEQERKYPEYTWDLTTIFKDDEAFEAAFKEVENELGK
                     EEQFKGHIGDSAETLYNALELEDTLGTKLEKVYVYAHLKQDQDTTNDKYTGMESRAHQ
                     LIIKFSSAWSFLVPEILQIDEDKIQSFVNSYDKLQKFAFDLKLINEKRPHILDAETEK
                     LLTEAQDALSTPSNVYGMFSNADLVFEDAIDKDGNAHPLTQGTFIKYLETDDRKLRES
                     AFRNVYKAYGAHNNTLGATLAGEVKKNVFNARTHNYKTAREKALSNNHIPENVYDNLV
                     KTVHKYLPLLHRYTELRKELLGLDDLKMYDLYTPLIKDIKFEMPYEEAKEWMLKALEP
                     MGEEYLNVVKEGLNNRWVDVYENKGKRSGGYSSGAHLTNPFILLNWSNTISDLYTLVH
                     EFGHSAHSYFSRKFQPSNSSDYTIFVAEVASTCNEALLSDYMDKHLDDEKRLLLLNQE
                     LERFRATLFRQTMFAEFEHKIHAIEEAGEPLTPTRMNEEYAKLNKLYFGDSVETDEDI
                     SKEWSRIPHFYMNYYVYQYATGYSAAQSLSHQILTEGKPAVDRYINEFLKKGSSNYPI
                     EILKNAGVDMTTPEPIEQACEVFEQKLNAFEKLMKA"
     gene            complement(2285..3270)
                     /locus_tag="O0293_002461"
                     /pseudo
     CDS             complement(2285..3270)
                     /locus_tag="O0293_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499489.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
     gene            complement(3391..4110)
                     /gene="mecA"
                     /locus_tag="O0293_002462"
     CDS             complement(3391..4110)
                     /gene="mecA"
                     /locus_tag="O0293_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HCY0281322.1"
                     /translation="MRIERVDDTTVKLFITYSDIEARGFSREDLWTNRKRGEEFFWSM
                     MDEINEEEDFVVEGPLWIQVHAFEKGVEVTISKSKNEDMMNMSDDDATDQFDEQVQEL
                     LAQTLEGEDQLEELFEQRTKEKEAQGSKRQKSSARKNTRTIIVKFNDLEDVINYAYHS
                     NPITTEFEDLLYMVDGTYYYAVYFDSHVDQEVINDSYSQLLEFAYPTDRTEVYLNDYA
                     KIIMSHNVTAQVRRYFPETTE"
BASE COUNT         1291 a          725 c          532 g         1589 t
ORIGIN      
        1 aacgaggtct ttttactgac tcttaatttt gcaacattaa attattagaa cgtaatgtta
       61 cacatcgatc taaatatatt agtgatattt ttcgaattta accagttcga aaattataca
      121 aattataaat ttggtacata atagacatat atgaatcgtt aatgtataaa aagtgcatat
      181 ttaatacatt tttatacaac ttcacttccc tcttattaaa aaagaacata aaaaaacgtc
      241 gtgtctgaat acacgacgct aaacaaaggg gatgggagaa atttttcact tcaaacaaag
      301 gggtatgttt gttatgtgat taatttcata ttcataatat aacatgctat ctctaccatt
      361 tcaacctttt aatagtactt aatttaaatt gtcacaatct gttcataatg taagcgaata
      421 caatagtact aagctttcat taatttttca aaagcgttca atttttgttc aaaaacttca
      481 caagcttgtt caattggttc aggtgttgtc atatctacac cagcattttt taatatctca
      541 attggataat ttgagctacc ttttttcaag aattcattaa tatatctatc tactgctggc
      601 ttaccttctg ttaaaatttg atgacttaag ctttgagctg cactgtaacc agttgcgtat
      661 tgatatacat aataattcat atagaagtgt ggaatacgtg accattcctt actaatatct
      721 tcatcagttt ctacagaatc accgaagtat aatttattta atttggcata ttcttcattc
      781 attctagttg gcgttaatgg ttcacctgct tcttcaattg catgaatttt atgctcaaat
      841 tctgcgaaca ttgtttgtcg gaataatgta gctctgaaac gttctaattc ttggtttaat
      901 aataataagc gtttttcatc atcaagatgt ttatccatat aatcacttaa aagtgcttcg
      961 ttacaagttg atgcaacttc agcgacaaaa atagtgtagt cactagaatt tgacggttgg
     1021 aattttctac tgaagtaact atgtgctgaa tgcccaaatt catgaactaa tgtgtataag
     1081 tctgaaatag tattagacca gttaagtaga ataaatggat tagttaaatg tgcacctgat
     1141 gaatagccac ctgaacgttt acctttattc tcatagacat cgacccaacg attgtttaag
     1201 ccttctttaa ctacatttaa atattcttca cccattggtt ctaatgcttt taacatccac
     1261 tctttagctt cttcataagg catttcaaac ttaatatctt taattaatgg tgtatataaa
     1321 tcatacattt tcaagtcatc taaacctagc aattctttgc gcaattcagt atatctatgt
     1381 agcaatggta aatatttatg tacagttttt actagattgt catatacatt ttctggaata
     1441 tgattattac tcaatgcttt ttctcttgca gttttgtaat tgtgtgtacg agcattaaat
     1501 acatttttct tcacttcacc tgctagcgta gcgccaagcg tattattatg agcaccatat
     1561 gctttatata catttctaaa agcactttct cttagtttgc gatcatctgt ttctaaatac
     1621 ttaataaatg taccttgtgt taacgggtgt gcatttccat ctttatctat cgcatcttca
     1681 aatactaaat cagcgttgct aaacataccg tatacatttg atggcgttga taacgcgtcc
     1741 tgcgcttctg ttaataactt ttcagtttca gcatctaaaa tatgaggacg tttttcatta
     1801 atcaacttca aatcaaatgc gaatttttgt aatttatcat atgaatttac aaatgattga
     1861 attttatctt catcaatttg taaaatctct ggcactaaga aactccatgc cgagctaaat
     1921 ttaataatta attgatgtgc tcttgactcc ataccagtat acttgtcgtt cgttgtatct
     1981 tggtcttgtt ttaggtgcgc gtatacatat actttttcta atttagtacc taatgtatct
     2041 tctaattcta acgcattgta taatgtctca gcactatcac caatgtgtcc tttaaattgt
     2101 tcttctttgc ctaactcatt ttcaacttct ttaaatgcag cctcaaaagc ttcatcatct
     2161 ttgaaaattg ttgttaagtc ccatgtatat tcaggatatt tacgttcctg ttcttctctc
     2221 gataattgtt gactcatatg tagtaaacct cctaataaaa tataattgat ataattttct
     2281 cattttagaa caaaatttgc acgtcattac ctttatctga acaaatattt aaataattgt
     2341 taataagttt ttctataatt gctgttttac tttctagagc aacaataaat tttttcagtt
     2401 taaaataatt aagattgtca tttactaatt tgctttttcc attctttaac cataaatcaa
     2461 cttgtaattg ccactcaata ggatgatttt taatataaat ttgctctgga aaaatataac
     2521 cataattgtg cactacttct tgatcagtta actgtaattg atacattgca cttaaagtgg
     2581 gttctaaaac agaattttgc cagcgacaat atttgatata ttgattaatt gcgaactttg
     2641 ataatttata aacagcatga taatcacaag gcgcctcatt aaacaactca ctaattttgg
     2701 catttctttt ttcagcgaca tatttgtgcc cgcctaaata ttgtatttgt tgaaacatca
     2761 ttatttgttt tttctctaaa ttgaacgtat gaagttttcg agtgaatgga ttaataaaca
     2821 tactttgaaa atgattgaac ttcactttat tatgacaata atcaacatca tttaataacc
     2881 aacttacttt atatcccatt ttctttaaac cttccgttcg ttgaagaatc tgtttataag
     2941 gaattggcga atactgtagc tcaataacat atttattatt aatcaaaata tccggaattt
     3001 gttttatttc ttttaaaatg gctctatctc tactttacat ccttgttgct gtatcatctg
     3061 tgctaaaatc aatttacttt tataatgttt aatcgtttca ttattaaaac atttttgttc
     3121 cgctaaatgt ttatgtgcaa aatgactaat tactttgagc ccacgcttta aaataacttg
     3181 cttgccacac accggacaaa aatattgtgt ctttctcaat gcagtagttg ctaatacgcg
     3241 ttccttttct tcatttaaag ctactaacat atcttcacct caaaatatta cacgtaagtt
     3301 taactactat tactttttca gcttttgaaa tcaataaaaa aagagactcc aaagagtctc
     3361 ctttcatagt gcgttgctta attaatatcg ttattcagtt gtctctggaa aataacgtcg
     3421 aacttgagct gttacgttat gactcataat tattttagca tagtcattta aataaacttc
     3481 tgttctgtct gttggataag caaattcaag caattgactg taactatcat taatgacttc
     3541 ttgatcaaca tgactatcaa aatatacagc ataataataa gtaccatcaa ccatatataa
     3601 caaatcttca aactctgtag ttattggatt gctatgatat gcataattaa taacatcttc
     3661 taaatcgtta aatttcacaa tgattgttct tgtattttta cgtgctgaag acttttgacg
     3721 tttagaacct tgagcttctt tttcttttgt tcgttgctcg aataattctt ctaattgatc
     3781 ttcaccttct aatgtttgag ctaacaattc ttgaacttgt tcatcaaatt gatcagttgc
     3841 atcatcatca gacatattca tcatatcttc atttttagat ttagaaattg tgacttcgac
     3901 acctttttca aaggcatgta cttgaatcca taatggacct tctacaacaa aatcttcttc
     3961 ttcgttaatt tcatccatca ttgaccaaaa gaattcttcg ccacgtttgc gatttgtcca
     4021 taaatcttca cgactaaatc cacgggcctc gatatcgcta tatgttataa acaattttac
     4081 agttgtatca tctactcgtt ctattctcat atcatctcac tccttacagt cgatgaa
//
LOCUS       DAKTIO010000197         4136 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.202, whole genome shotgun sequence.
ACCESSION   DAKTIO010000197 DAKTIO010000000
VERSION     DAKTIO010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.202"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..1637
                     /locus_tag="O0293_002463"
     CDS             <1..1637
                     /locus_tag="O0293_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA2/NAM7 family helicase"
                     /protein_id="HCY0281323.1"
                     /translation="SFSKIEQVINSEKTSIEEQVYHLDNETLRDNKEIEDLDNRINYI
                     VKQIETLNELIKSIKESNKGFINKLKAMFNSEEDESYKDHNKEKQQLLTQQLELEKCK
                     KNKHEDLVSKLKEKEKLIKQLTKVQLQLDELNSQLQELEAYRIESKITIPEKDFWSDN
                     NYDERQVTNLWTSDELQYRRAMLFLRAMILHKLLLIANNTTIYYAINDFKDRRKLIDA
                     NPDKVHNAWNVMHLIFPVVSTTFASFKSMYGGIPKDFIDYLFIDEAGQAIPQAAVGAL
                     YRSKKVVAVGDPIQIEPVVTLESHLIDNIRKNYHVPEYLVSKEASVQSVADNANQYGF
                     WKSDATDSNQKTWIGIPLWVHRRCLKPMFTIANQIAYNNKMVLPSNITKVGKTGWYDV
                     KGNAVQKQFVKEHGEKVVGLLADDWIEAIKEGKNEPSSFVISPFSAVQQQIKRMLKQQ
                     LPTRIDIERTKINQWVDKSIGTVHTFQGKEAQKVYFVIGTDNTQDGAVNWSCEKPNLL
                     NVAVTRAKKEFYVIGDMQRIQMKPFYETIFKERNVK"
     gene            1694..2176
                     /locus_tag="O0293_002464"
     CDS             1694..2176
                     /locus_tag="O0293_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281324.1"
                     /translation="MEKFNNWILNAISGSQTDKNETTEELKGAKFIILYAYSMLVLLA
                     LVISNIFIHILEPKLSITTQIIIVLILIEALIGLRFLKAYDVKRGKDKENKKNSKDFV
                     KLKSILVAILFTSLALTAGTVADIYGFTDLGNTRSDLIVWSIGGIIFGLVCYTMEDKR
                     "
     gene            2381..3367
                     /locus_tag="O0293_002465"
     CDS             2381..3367
                     /locus_tag="O0293_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase C"
                     /protein_id="HCY0281325.1"
                     /translation="MKKCIKTLFLSIILVVMSGWYHSAHASDSLSKSPENWMSKLDDG
                     KHLTEINIPGSHDSGSFTLKDPVKSVWAKTQDKDYLTQMKSGVRFFDIRGRASADNMI
                     SVHHGMVYLHHELGKFLDDAKYYLSAYPNETIVMSMKKDYDSDSKVTKTFEEIFREYY
                     YNNPQYQNLFYTGSNANPTLKETKGKIVLFNRMGGTYIKSGYGADTSGIQWADNATFE
                     TKINNGSLNLKVQDEYKDYYDKKVEAVKNLLAKAKTDSNKDNVYVNFLSVASGGSAFN
                     STYNYASHINPEIAKTIKANGKARTGWLIVDYAGYTWPGYDDIVSEIIDSNK"
     gene            3588..>4136
                     /locus_tag="O0293_002466"
     CDS             3588..>4136
                     /locus_tag="O0293_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0281326.1"
                     /translation="MMKRLNKLVLGIIFLFLVISITAGCGIGKEAEVKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIINSEMVIQPNNEDMVAKGMVLYMNRNTKTT
                     NGYYYVDVTKDEDEGKPHDNEKRYPVKMVDNKIIPTKEIKDEKIKKEIENFKFFVQYG
                     DFKNLKNYKDGDISYNPEVPSYS"
BASE COUNT         1624 a          502 c          775 g         1235 t
ORIGIN      
        1 agtctttctc taagattgaa caggttatta atagtgaaaa aacaagtatt gaagaacagg
       61 tatatcattt agataatgaa acgttacgag acaataaaga aatagaagat ttggataatc
      121 gaattaatta tattgttaag caaatagaaa ctttaaatga gttaattaaa tccatcaaag
      181 aaagcaacaa aggttttatt aacaaactga aagcgatgtt taattcagaa gaagatgaaa
      241 gctataaaga tcataataaa gagaagcaac aattattaac acaacagtta gagttagaga
      301 aatgtaaaaa aaacaaacat gaagaccttg ttagcaaact aaaagaaaaa gagaaattaa
      361 ttaaacaatt aactaaagta cagttgcaat tagacgagtt aaattcacag ttacaagagt
      421 tagaagcata tcgtattgag tcaaaaatta caattccaga aaaagatttt tggagtgaca
      481 acaattatga tgagcgccaa gttactaatc tgtggacgag tgacgaactt caatacagac
      541 gtgccatgct ctttttaaga gcaatgatat tgcataaatt attattgatt gctaataata
      601 caactattta ttatgcgatt aatgatttta aagatagaag gaaattaatt gatgcaaatc
      661 cagataaagt acacaacgca tggaatgtga tgcatttaat atttccagta gttagtacga
      721 cgtttgcaag ctttaaatct atgtatgggg gcataccaaa agatttcata gactacttat
      781 ttattgatga agcaggacaa gcaatacctc aagcagctgt gggagcatta tatcgttcaa
      841 aaaaagttgt agctgtaggt gatccgattc aaatagaacc ggttgtgact ttagaaagtc
      901 atttaattga taacattcgt aaaaattatc atgttccgga atatctagtt tctaaagaag
      961 cttctgtgca gtctgttgca gacaacgcca atcaatatgg tttttggaaa tctgatgcta
     1021 ctgatagtaa tcaaaaaacc tggataggca tacctttatg ggtgcacaga cgatgtttaa
     1081 aacctatgtt cacgatagct aaccaaatcg cttataataa taaaatggtg ttgccaagta
     1141 atattacaaa agtaggtaaa acaggttggt atgacgttaa aggaaacgca gttcaaaaac
     1201 aatttgtgaa agagcatggt gaaaaagtag tgggattatt agctgatgat tggattgaag
     1261 caattaagga aggtaaaaat gaaccgagct catttgtaat atcgcctttt tcagcagtac
     1321 agcaacagat taaacgtatg ttaaagcaac aactaccgac tagaattgat attgaacgta
     1381 caaaaattaa tcaatgggtc gataaatcca ttggtactgt tcatactttt caaggtaaag
     1441 aggctcagaa ggtgtatttt gtaataggta ctgataatac ccaagatggt gctgtgaact
     1501 ggtcatgcga aaaaccaaac ttgttaaacg ttgcagtgac aagagctaag aaagagtttt
     1561 atgtaattgg cgacatgcaa agaatacaga tgaaaccatt ttatgagacg atttttaaag
     1621 aaagaaatgt aaaataacat acaaaaaagt atatagagga agttatacat tttaaaagga
     1681 gcaaaattga ataatggaga aatttaacaa ctggatatta aatgcaataa gtggatctca
     1741 aacagacaag aatgaaacaa ctgaagaatt aaaaggggca aaatttatca ttttatatgc
     1801 atattcaatg ctcgttttgc ttgcgttagt aatttctaac atattcattc acattttgga
     1861 gcctaaacta tcaatcacca ctcaaatcat catcgttttg attttaattg aagcactaat
     1921 tggactgcgt ttcttgaaag cgtacgatgt taagcgtggc aaagataaag aaaataagaa
     1981 aaatagtaag gatttcgtta aactaaaatc aattttagta gcaattttat ttacatcatt
     2041 ggcgctgaca gcaggtactg tagctgatat atacggtttc actgacttag gaaatactag
     2101 aagtgattta atcgtttgga gcataggtgg tattatattt ggcctcgtat gttacacaat
     2161 ggaagataaa agataacgat aaggagctgg cgattataaa gctagctcct tttttaactt
     2221 atatatgtaa agaactatcc taagggtttt taatcatatg tcaataattt ctataataca
     2281 ttattaaaac atcaattaaa taagttttaa aattttacac atattgttat ttaaaaaaga
     2341 tgtataatta atgtattaaa tatagaaaga agttgatatt atgaaaaagt gtattaagac
     2401 tttgttttta agtatcattt tagtagtgat gagtggttgg tatcattcag cacatgcgtc
     2461 agattcgttg agtaaaagtc cagaaaattg gatgagtaaa cttgatgatg gaaaacattt
     2521 aactgagatt aatataccgg gttcacatga tagtggctca ttcactttaa aggatccagt
     2581 aaaatcagtt tgggcaaaga ctcaagataa agattacctt acccaaatga agtcgggagt
     2641 caggtttttt gatattagag gtagagcaag tgctgataat atgatttcag ttcatcacgg
     2701 catggtttat ttgcatcatg aattaggaaa atttctcgat gatgctaaat attacttgag
     2761 tgcttatcca aacgaaacaa ttgtgatgtc tatgaaaaag gactacgata gcgattctaa
     2821 agttacgaag acatttgaag aaatttttag agaatattat tataataacc cgcaatatca
     2881 gaatcttttt tacacaggaa gtaatgcgaa tcctacttta aaagaaacga aaggtaaaat
     2941 tgtcctattc aatagaatgg ggggtacgta cataaaaagt ggttatggtg ctgacacgtc
     3001 aggtattcaa tgggcagaca atgcgacatt tgaaacgaaa attaataatg gtagcttaaa
     3061 tttaaaagta caagatgagt ataaagatta ctatgataaa aaagttgaag ctgttaaaaa
     3121 tttattggct aaagctaaaa cggatagtaa caaagacaat gtatatgtga atttcttgag
     3181 tgtagcgtct ggaggcagcg catttaatag tacttataac tatgcatcac atataaatcc
     3241 tgaaattgca aaaacgatta aagcaaatgg gaaagctaga acgggttggc tgattgttga
     3301 ctatgcagga tatacgtggc ctggatatga tgatatcgta agtgaaatta tagatagtaa
     3361 taaataagga ttcaataatg atattaagac gagtatgaaa atagttagat tctaattatt
     3421 ttcactactc gtttttattt tgaaaataag taataattca acaatattat aaattgaaca
     3481 gattgtttgt gaaatttttg ataatattaa agtgaaaaag tgttataaat tgataaatat
     3541 atgtaattaa caaaaacaaa tcattttaaa aagaagagag ttgtaagatg atgaaacgat
     3601 taaacaaatt agtgttaggc attatttttc tgtttttagt cattagtatc actgctggtt
     3661 gtggcatagg taaagaagcg gaagttaaga aaagctttga aaaaacattg agtatgtacc
     3721 ctattaaaaa tctagaggat ttatacgata aggaaggcta tcgtgatgat cagtttgata
     3781 aaaatgataa aggtacatgg attataaatt ctgaaatggt tattcaacct aataatgaag
     3841 atatggtagc taaaggcatg gttctatata tgaatagaaa taccaaaaca acaaatggtt
     3901 actactatgt cgatgtgact aaggacgagg atgaaggaaa accgcacgac aatgaaaaaa
     3961 gatatccggt taaaatggtc gataataaaa tcattccaac aaaagaaatt aaagatgaaa
     4021 aaataaaaaa agaaatcgaa aactttaagt tctttgttca atatggcgac tttaaaaatt
     4081 tgaaaaatta taaagacgga gatatttcat ataatccaga ggtgccgagt tattcg
//
LOCUS       DAKTIO010000198         4068 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.203, whole genome shotgun sequence.
ACCESSION   DAKTIO010000198 DAKTIO010000000
VERSION     DAKTIO010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.203"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            110..3072
                     /locus_tag="O0293_002467"
                     /pseudo
     CDS             110..3072
                     /locus_tag="O0293_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="E domain-containing protein"
     gene            3456..>4068
                     /locus_tag="O0293_002468"
     CDS             3456..>4068
                     /locus_tag="O0293_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0281327.1"
                     /translation="MLYLEVLKNRNFTYLLIGNFLRRSCFVLFSLQIIWFTVELTNQS
                     SLKLSMMVMSQTLPFIIFGIFGGAYSDKHNKKKILYLSDLILSLIIIIIPLLAITSNL
                     NYLTLLTISTAITIINCYTDPAFRAILPEIIDEEHLATSNALIDSLQRGSNIILPALI
                     GVIVILVGNVGVFFICSILLFLGFIFNALLKYTNNNMIDRHSKE"
BASE COUNT         1639 a          576 c          794 g         1059 t
ORIGIN      
        1 tttataaaga taagtgttat attccattgt gcaactccta ttgtatttgt aaaattacat
       61 gtaaattcac tgtaagcaaa gtggaaaata tggaaaaagg agtatgcaaa tgagagataa
      121 gaaaggaccg gtaaataaaa gagtagattt tctatcaaat aaattgaata aatattcaat
      181 aagaaaattt acagttggaa cagcatctat tttaattggc tcactaatgt atttgggaac
      241 tcaacaagag gcagaagcag ctgaaaacaa tattgagaat ccaactacat taaaagataa
      301 tgtccaatca aaagaagtga agattgaaga agtaacaaac aaagacactg caccacaggg
      361 tgtagaagct aaatctgaag taacttcaaa caaagacaca atcgaacatg aaccatcagt
      421 aaaagctgaa gatatatcaa aaaaggagga tacaccaaaa gaagtagctg atgttgctga
      481 agttcagccg aaatcgtcag tcactcataa cgcagagaca cctaaggtta gaaaagctcg
      541 ttctgttgat gaaggctctt ttgatattac aagagattct aaaaatgtag ttgaatctac
      601 cccaattaca attcaaggta aagaacattt tgaaggttac ggaagtgttg atgtacaaaa
      661 aaaaccaaca gatttagggg tatcagaggt aaccaggttt aatgttggta atgaaagtaa
      721 tggtttgata ggagctttac aattaaaaaa taaaatagat tttagtaagg atttcaattt
      781 taaagttaga gtggcaaata accatcaatc aaataccaca ggtgctgatg gttgggggtt
      841 cttatttagt aaaggaaatg cagaagaata tttaactaat ggtggaatcc ttggggataa
      901 aggtctggta aattcaggcg gatttaaaat tgatactgga tacatttata caagttccat
      961 ggacaaaact gaaaagcaag ctggacaagg ttatagagga tacggagctt ttgtgaaaaa
     1021 tgacagttct ggtaattcac aaatggttgg agaaaatatt gataaatcaa aaactaattt
     1081 tttaaactat gcggacaatt caactaatac atcagatgga aagtttcatg ggcaacgttt
     1141 aaatgatgtc atcttaactt atgttgcttc aactggtaaa atgagagcag aatatgctgg
     1201 taaaacttgg gagacttcaa taacagattt aggtttatct aaaaatcagg catataattt
     1261 cttaattaca tctagtcaaa gatggggcct taatcaaggg ataaatgcaa atggctggat
     1321 gagaactgac ttgaaaggtt cagagtttac ttttacacca gaagcgccaa aaacaataac
     1381 agaattagaa aaaaagttga agagattcca ttcaagaaag aacgtaaatt taatccggat
     1441 ttagcaccag ggacagaaaa agtaacaaga gaaggacaaa aaggtgagaa gacaataacg
     1501 acaccaacac taaaaaatcc attaactgga gtaattatta gtaaaggtga accaaaagaa
     1561 gagattacaa aagatccgat taatgaatta acagaatacg gacctgaaac aatagcgcca
     1621 ggtcatcgag acgaatttga tccgaagtta ccaacaggag agaaagagga agttccaggt
     1681 aaaccaggaa ttaagaatcc agaaacagga gacgtagtta gaccgccggt cgatagcgta
     1741 acaaaatatg gacctgtaaa aggagactcg attgtagaaa aagaagagat tccattcgag
     1801 aaagaacgta aatttaatcc ggatttagca ccagggacag aaaaagtaac aagagaagga
     1861 caaaaaggtg agaagacaat aacgacgcca acactaaaaa atccattaac tggagaaatt
     1921 attagtaaag gtgaatcgaa agaagaaatc acaaaagatc cgattaatga attaacagaa
     1981 tacggaccag aaacgataac accaggtcat cgagacgaat ttgatccgaa gttaccaaca
     2041 ggagagaaag aggaagttcc aggtaaacca ggaattaaga atccagaaac aggagatgta
     2101 gttagaccac cggtcgatag cgtaacaaaa tatggacctg taaaaggaga ctcgattgta
     2161 gaaaaagaag aaattccatt cgagaaagaa cgtaaattta atcctgattt agcaccaggg
     2221 acagaaaaag taacaagaga aggacaaaaa ggtgagaaga caataacgac gccaacacta
     2281 aaaaatccat taactggaga aattattagt aaaggtgaat cgaaagaaga aatcacaaaa
     2341 gatccagtta atgaattaac agaattcggt ggcgagaaaa taccgcaagg tcataaagat
     2401 atctttgatc caaacttacc cacagatcaa acggaaaaag taccaggtaa accaggaatc
     2461 aagaatccag acacaggaaa agtgatcgaa gagccagtgg atgatgtgat taaacacgga
     2521 ccaaaaacgg gtacaccaga aacaaaaaca gtagagatac cgtttgaaac aaaacgtgag
     2581 tttaatccaa aattacaacc tggtgaagag cgagtgaaac aagaaggaca accaggaagt
     2641 aagacaatca caacaccaat cacagtgaac ccattaacag gtgaaaaagt tggcgagggt
     2701 caaccaacag aagagatcac aaaacaacca gtagataaga ttgtagagtt cggtggagag
     2761 aaaccaaaag atccaaaagg acctgaaaac ccagagaagc cgagcagacc aactcatcca
     2821 agtggcccag taaatcctaa caatccagga ttatcgaaag acagagcaaa accaaatggc
     2881 ccagttcatt caatggataa aaatgataaa gttaaaaaat ctaaaattgc taaagaatca
     2941 gtagctaatc aagagaaaaa acgagcagaa ttaccaaaaa caggtttaga aagcacgcaa
     3001 aaaggtttga tctttagtag tataattgga attgctggat taatgttatt ggctcgtaga
     3061 agaaagaatt aaaataattc ataatttaaa taatagttga tttgcattca ctatatttag
     3121 tttgttaaaa acaacctaga atatgatgag aatgatatac accccaaaag ttggattgaa
     3181 agacctaact ttaaggggtg tatttttgtt ataaatttga tttttttgat aatattttca
     3241 tatttaatat aatcataatt agtagaatat aacctctgag tagtcatttt gggggttatg
     3301 tatggtgttt caagtagtat gtatatttcg aaataattga tatttaactg actaaattat
     3361 attagaatta attgcgcttt ggataaagag agtttagaag aaggtctaaa aaagctggtg
     3421 gatgcattca attattttta tgagaggtga taaatatgtt gtatttagaa gttttgaaaa
     3481 atagaaactt tacatattta ttgattggta atttcttacg tcggagttgt tttgtgctgt
     3541 tttcgttaca aataatttgg tttactgttg aattgacaaa ccaatcatct ttaaaattat
     3601 caatgatggt tatgagtcaa acacttccat ttattatatt tggtattttc ggtggcgcat
     3661 attctgataa acataataaa aagaaaatac tttatctatc tgatcttata ctaagtttga
     3721 ttataataat tattccttta cttgcaataa catcaaattt aaattatctg acgcttctaa
     3781 ccatatctac agctatcacc attattaact gctatacgga tcctgcgttt agggcaattc
     3841 ttcctgaaat tattgatgag gagcatttag caacaagtaa tgcgttaatc gatagtttgc
     3901 aaagaggctc aaatattatt ttacctgctt taattggtgt cattgtaata ctagttggta
     3961 atgttggcgt cttttttatt tgtagtatat tgctattttt aggatttatt tttaatgcac
     4021 ttttaaaata tacaaataac aacatgattg atagacactc aaaagaag
//
LOCUS       DAKTIO010000199         4055 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.204, whole genome shotgun sequence.
ACCESSION   DAKTIO010000199 DAKTIO010000000
VERSION     DAKTIO010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.204"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            19..474
                     /locus_tag="O0293_002469"
     CDS             19..474
                     /locus_tag="O0293_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0281328.1"
                     /translation="MVKHLQDHIQFLEQFINNVNALTAKMLKDLQNEYEISLEQSNVL
                     GMLNKEPLTISEITQRQGVNKAAVSRRIKKLIDAKLVKLDKPNLNIDQRLKFITLTDK
                     GRAYLKERNAIMTDIAQDITNDLNSEDIENVRQVLEVINHRIKTYSNHK"
     gene            714..2096
                     /locus_tag="O0293_002470"
     CDS             714..2096
                     /locus_tag="O0293_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281329.1"
                     /translation="MKSCPKCGQQAQDDVQICTQCGHKFDSRQALYRKSTDEDIQTNN
                     IKMRKMVPWAIGFFILILIIILFFLLRNFNSPEAQTKILVNAIENNDKQKVATLLSTK
                     DNKVDSEEAKVYINYIKDEVGLKQFVSDLKNTVHKLNKSKTSVASYIQTRSGQNILRV
                     SKNGTRYIFFDNMSFTAPTKQPIVKPKEKTKYEFKSGGKKKMVIAEANKVTPIGNFIP
                     GTYRIPAMKSTENGDFAGHLKFDFRQSNSETVDVTEDFEEANISVTLKGDTKLNDSSK
                     KVTINDHEMAFSSSKTYGPYPQNKDITISASGKAKDKTFTTQTKTIKASDLKYNTEIT
                     LNFDSEDIEDYVEKKEKEENSLKNKLIEFFAGYSLANNAAFNQSDFDFVSSYIKKGSS
                     FYDDVKKRVSKGSLMMISSPQIIDAEKHGDKITATVRLINENGKQVDKEYELEQGSQD
                     RLQLIKTSEK"
     gene            2127..2255
                     /locus_tag="O0293_002471"
     CDS             2127..2255
                     /locus_tag="O0293_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281330.1"
                     /translation="MEYKQHPFKVFTISVNYFEGIVFCGKIYEVLRFISFYTFYKL"
     gene            2365..3573
                     /locus_tag="O0293_002472"
     CDS             2365..3573
                     /locus_tag="O0293_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HCY0281331.1"
                     /translation="MNDLTHFNKRSPIFIIILGSLTAIGALSIDMFLPGLPDIRHDFQ
                     TTTSNAQLTLSMFMIGLAFGNLFAGPISDSTGRRKPLIIAMIIFTLASLGIVFVHNIW
                     LMVALRFLQGVTGGAAAVISRAIASDMYSGNELTKFMALLMLVNGIAPVVAPTIGGII
                     LNYSVWRMVFVILTIFGFVMVIGSLLKVPESLTVTNRESSSGLKTMFKNFKILLKTPR
                     FVLPMLIQGMTFVILFTYISASPFIIQKIYGMTAIQFSWMFAGIGITLIISSQLTGYL
                     VDFIDSQKLMRGMTMIQIIGVILVTIVLLNHWNFWILAIGFIILIAPVTGVATLGFTI
                     AMDESSSGRGSSSSLLGLVQFLFGGVASPLVGVKGEDNPIPYIIIIIATAVILIILQI
                     YNMKVFKTNR"
     gene            complement(3803..>4055)
                     /locus_tag="O0293_002473"
     CDS             complement(3803..>4055)
                     /locus_tag="O0293_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HCY0281332.1"
                     /translation="FFKDILVTYPMKTLFIDYINLARDYYQQIMNDYSTTVTNKQLYV
                     TYIIGGQAGVFINWLSNGCNESPEEVADILLANTIKLQN"
BASE COUNT         1486 a          518 c          717 g         1334 t
ORIGIN      
        1 aattagggag aaataattat ggtaaaacat ttacaagacc atattcaatt tttagagcag
       61 tttataaata acgttaacgc attaactgca aaaatgttga aagatttaca aaatgaatat
      121 gaaatttcat tagagcagtc taacgtatta ggtatgttaa ataaagaacc tttgacaatt
      181 agtgaaatca cgcaaagaca aggtgtaaat aaggccgcag taagccgacg aattaaaaag
      241 ttaatcgatg ctaaattagt taagttagat aaaccaaatt taaatattga tcaacgtttg
      301 aaattcataa ccttaactga caaaggtaga gcatatttga aagaacgtaa tgcgattatg
      361 acagatattg cgcaagatat tactaatgat ttaaattctg aagatattga aaatgtaagg
      421 caagtattag aagtcatcaa tcatcgcatt aaaacatata gcaatcataa ataggttttt
      481 gaaggtgttc aaatataaga ctaaatatgt atgtattgaa caccttcttt ttagttataa
      541 ttgagagagt actttcataa ataatgtaat aaggtacaat tctaaagtaa agttaatcat
      601 ttcttgaatt gtattcttgg gataatattt gtaaaataat aatggtaaaa taatgaaata
      661 atttttgtga tatacatata acaattgaag aggaacagaa ttgaggtgaa agtatgaaat
      721 cttgcccgaa gtgcggacaa caagcacaag atgatgtaca gatatgtaca caatgtggac
      781 ataaatttga tagtcgtcaa gcattatata gaaagtcaac cgatgaagat atacaaacta
      841 ataacatcaa gatgagaaaa atggtgcctt gggcaattgg ttttttcatt ttaatcttaa
      901 ttattatatt gttcttttta ctaagaaact tcaattcacc tgaagcgcaa actaaaatat
      961 tagtgaatgc gattgagaat aatgataaac aaaaagtagc aacattatta agtactaaag
     1021 ataataaagt agattctgaa gaagcaaaag tatacattaa ctatatcaaa gatgaagttg
     1081 ggcttaagca atttgtcagc gaccttaaaa atacggtaca taaattgaat aagagtaaga
     1141 ccagcgtagc ttcttatatt caaaccagat ctggtcaaaa tatattacgt gtaagtaaaa
     1201 atggcacacg ttatatcttt ttcgataata tgagctttac tgcacctacc aagcaaccaa
     1261 ttgttaaacc gaaagaaaaa acaaaatatg agtttaaatc tggtggtaag aaaaagatgg
     1321 ttatagctga agcaaataaa gtgacgccaa taggtaattt tataccgggg acatatagaa
     1381 ttccagctat gaaatcaact gagaacggtg attttgcagg ccatttaaaa tttgatttta
     1441 gacaaagtaa ttctgaaacg gtagatgtta ctgaagattt tgaagaagca aatatatctg
     1501 taactttaaa aggcgataca aaattaaatg atagttctaa aaaagtaact ataaatgacc
     1561 atgaaatggc attttcaagt tccaaaacgt atggtccata tccacaaaat aaagatatta
     1621 ccatttcagc ttcaggtaaa gcgaaagata aaacatttac aacacagacg aagacgatta
     1681 aagctagcga tttaaaatac aatacagaga taactttgaa ttttgacagt gaagatatcg
     1741 aagactatgt tgaaaagaaa gaaaaagaag aaaacagctt gaagaacaaa ttgatagaat
     1801 tctttgctgg atattcttta gcgaataatg ctgcgtttaa tcagtcggat tttgattttg
     1861 tatcatcata tataaaaaaa ggatcatctt tttatgatga tgtaaagaag cgtgtatcta
     1921 aaggaagttt aatgatgatt agttcaccac aaattataga tgctgaaaaa catggtgata
     1981 agattaccgc aaccgtaaga ttaataaatg aaaacggtaa gcaagtagat aaagaatatg
     2041 agcttgaaca aggctcgcaa gaccgcttgc aattaatcaa gacatcagaa aaatagagta
     2101 taaaacttaa ataagatgca tgtgtcatgg aatacaaaca acatcctttc aaagtattta
     2161 caattagtgt gaattacttt gaagggattg ttttttgtgg aaaaatttat gaagtgctga
     2221 ggtttatatc attttatacc ttttataagt tgtaatgatg ttcatgaaac ttgtgactaa
     2281 tgctaatata taaaagagca tatttaaatt tagtttctgt tcgtgatagt tataaactaa
     2341 tcattatata aaggagatag tcatatgaat gatttaacgc attttaacaa gcgttcaccg
     2401 atatttatta ttattttagg atctttgacg gccattggag cattatcgat agatatgttt
     2461 ttgcctggat taccagatat tagacatgat tttcaaacaa cgacgtctaa cgcacaatta
     2521 acattatcga tgtttatgat tggtttagcg tttggtaatt tgtttgctgg tccaatttca
     2581 gattcaactg ggcgcagaaa gccgcttatt atagctatga ttatttttac attagcaagt
     2641 ttaggtattg tttttgtaca taatatttgg ctgatggttg cattaagatt tcttcaagga
     2701 gtgacaggtg gcgcagcagc agttatttca agagctatag caagtgatat gtatagtggt
     2761 aatgaactaa ctaagtttat ggcattattg atgcttgtta atggtattgc accagttgtt
     2821 gcgccaacaa tagggggcat aattttaaat tattctgtct ggcgtatggt ctttgtcatt
     2881 ctgactatct ttggcttcgt catggtcata ggttctttat taaaagtacc tgaatcatta
     2941 acagtgacaa atcgtgagtc aagtagtgga ttaaagacaa tgtttaaaaa cttcaaaata
     3001 ttattgaaga cgccacgttt tgtattacca atgctcattc aaggtatgac atttgtaata
     3061 ttatttactt atatttctgc gtctccgttt attattcaaa aaatttatgg catgactgca
     3121 atacaattta gttggatgtt tgcaggaatt ggtattacgc tcattatatc aagtcaactt
     3181 actgggtacc ttgttgattt tatagactca caaaaattga tgagaggcat gacgatgatt
     3241 cagattatcg gtgtaatttt agtgacgatt gtattgctta atcattggaa cttttggatt
     3301 ttagcgatag gatttatcat tttaatagca ccagtgacag gtgttgcgac acttggtttt
     3361 acaatagcta tggatgaaag tagtagtggt agagggagtt catcaagttt attaggttta
     3421 gtacagtttt tatttggtgg tgttgcatcg ccattagtgg gggtaaaagg tgaagataat
     3481 cctatacctt acataattat tattatagct acagcagtta tattaattat tttacagatt
     3541 tataatatga aggtatttaa aacaaatcga tgataaacat agtttcttct attatattga
     3601 attgaaaaat taaaaaacaa aacttgagct attcatagac ctatgaattt agcatcaaaa
     3661 ataaaaatgc cgagattatt atgtggaaat ctcggcattt ttatattgat agcaccgtta
     3721 catatgtact attatctatc catttggttt aactttatct agttgtttta tagcatttct
     3781 ttattagaat gaaaccgtat tattagtttt gaagtttaat cgtattagcc aataaaatgt
     3841 cagcaacttc ttcaggtgat tcattacaac cattcgaaag ccaattaata aacaccccag
     3901 cttgaccgcc tatgatatat gtaacataaa gctgtttatt ggttactgta gtactatagt
     3961 cattcataat ttgttgatag taatctctcg ctaaatttat atagtctatg aataatgttt
     4021 tcattggata ggtaactaaa atgtctttga aaaac
//
LOCUS       DAKTIO010000200         4048 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.205, whole genome shotgun sequence.
ACCESSION   DAKTIO010000200 DAKTIO010000000
VERSION     DAKTIO010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.205"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            155..958
                     /locus_tag="O0293_002474"
     CDS             155..958
                     /locus_tag="O0293_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0281333.1"
                     /translation="MKKIATATIATAGFATIAIASGNQAHASEQDNYGYNPNDPTSYS
                     YTYTIDAQGNYHYTWKGNWHPSQLNQDNGYYSYYYYNGYNNYNNYNNGYSYNNYSRYN
                     NYSNNNQSYNYNNYNSYNTNSYRTGGLGASYSTSSNNVQVTTTMAPSSNGRSISSGYT
                     SGRNLYTSGQCTYYVFDRVGGKIGSTWGNASNWANAAARAGYTVNNTPKAGAIMQTTQ
                     GAYGHVAYVESVNSNGSVRVSEMNYGYGPGVVTSRTISASQAAGYNFIH"
     gene            complement(975..1217)
                     /locus_tag="O0293_002475"
     CDS             complement(975..1217)
                     /locus_tag="O0293_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281334.1"
                     /translation="MTKLIENLKASIFVSTLTKNLSMYSRFDMPKFGCVNGCMLFILY
                     YRVNRIGENRNFEIFTNSIFFIEIKKANKALETLLA"
     gene            complement(1391..2791)
                     /gene="nhaC"
                     /locus_tag="O0293_002476"
     CDS             complement(1391..2791)
                     /gene="nhaC"
                     /locus_tag="O0293_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCY0281335.1"
                     /translation="MMKRKPTFLESISTMIVMVIVVVTGFVFFDIPIQVLLIIASAYA
                     TWIAKRVGLTWQDLEKGIAERLNTAMPAILIILAVGIIVGSWMFSGTVPALIYYGLDL
                     LNPSYFLISAFFISAVTSVATGTAWGSASTAGIALISIGNQLGIPPGMAAGAIIAGAV
                     FGDKMSPLSDTTNLAALVTKVNIFKHIHSMMWTTIPASIIGLLVWFIAGFQFKGHSND
                     KQIQTLLSELAQIYQINIWVWVPLIVIIVCLLFKMATVPAMLISSFSAIIVGTFNHHF
                     KMTDGFKATFSGFNESMIHQSHISSSVKSLLEQGGMMSMTQILVTIFCGYAFAGIVEK
                     AGCLEVLLTTISKGIHSVGSLICITVICCIALVFAAGVASIVIIMVGVLMKDLFEKYQ
                     VSRSVLSRTLEDSSTMVLPLIPWGTSGIYYTNQLHVSVEEFFIWTVPCYLCAIIAIIY
                     GFTGIGIKKSSNSRLT"
     gene            complement(2886..3968)
                     /locus_tag="O0293_002477"
     CDS             complement(2886..3968)
                     /locus_tag="O0293_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HCY0281336.1"
                     /translation="MKIAIVGSGNGAVTAAVDMVSKGHDVKLYCRNQSISKFQNAIEK
                     GGFDFNNEGDERFVKFTDISDDMEYVLKDAEIVQVIIPSSYIEYYADVMAEHVTDNQL
                     IFFNMAAAMGSIRFMNVLEDRHIETKPQLAEANTLTYGTRVDFENAAVDLSLNVRRIF
                     FSTYDRSCLNDCYDKVSSIYDHLVKEESLIKTNLENGNPEVHPGPTLLNVGRIDYAGE
                     FALYKEGITKHTVRLLHAIELERLNLGRRLGFELSTAKESRIERGYLERDKEDEPLNR
                     LFNTSPVFSQIPGPNHVESRYLTEDIAYGLVLWSSLGRVIDVPTPNIDAVIVIASTIL
                     ERDFFEEGLTVEEIGLDKLDLEKYLK"
BASE COUNT         1395 a          803 c          579 g         1271 t
ORIGIN      
        1 attacaaaac gttaacaaag cacacaagga cgctaatttc tgttatagtc tttcttgtcg
       61 tttaattacg agagataact tataacatta attttgtcac acggacatta cgaaaatatt
      121 ttagatttaa aatttttagg aggatatttt taaaatgaag aaaatcgcta cagctactat
      181 cgcaactgca ggattcgcta caatcgcaat tgcatcagga aatcaagctc atgcttctga
      241 gcaagataac tacggttata atccaaacga cccaacatca tatagctata cttacactat
      301 tgatgcacaa ggtaactacc attacacatg gaaaggtaac tggcatccaa gtcaattaaa
      361 ccaagataat ggctactaca gctattacta ctacaatggt tacaataact ataacaatta
      421 caacaacggt tatagctaca ataactacag ccgttacaac aactactcaa ataataatca
      481 atcatataac tacaataact ataatagtta caacacaaac agctaccgta ctggtggttt
      541 aggtgcaagc tacagcactt caagcaacaa tgttcaagta actacaacta tggctccatc
      601 atcaaatggc cgttcaatct caagtggtta tacttcagga cgtaacttat acacttctgg
      661 tcaatgtaca tactacgtat ttgatcgtgt aggtggtaaa atcggttcaa cttggggcaa
      721 tgcaagtaac tgggctaacg cagctgcaag agctggttac acagtgaaca atacaccaaa
      781 agctggtgca attatgcaaa caactcaagg tgcatacggt cacgttgcat acgttgaaag
      841 tgttaacagc aatggttcag taagagtttc agaaatgaac tatggttatg gcccaggtgt
      901 tgtaacttca cgtacaatct cagctagcca agctgctggt tataacttca ttcactaatt
      961 agtgatggct atgtttacgc caacaaggtt tcaagagcct tgttggcttt tttaatttct
     1021 atgaaaaaaa tcgaattggt aaaaatctca aaatttcgat tttcaccaat ccgattcact
     1081 ctgtaataaa gaataaacaa catacagcca ttgacacacc caaatttagg catgtcaaat
     1141 cggctataca ttgataaatt tttagttaaa gtacttacaa atatacttgc cttcaaattc
     1201 tcaattaatt tagtcattac aaatcatgac ataaaactta cattacatat atcaacatgt
     1261 tccgctacaa aacatattca gtatatgtat agaggatatt tattctaagt taaaaccttt
     1321 tcaaattata accgctacaa ttataaatga ttaaagtgtt tcaacatatt atatatattc
     1381 cacgctcaca ttaagttaaa cgtgaattcg atgacttttt aatacctatc cctgtaaaac
     1441 catagataat tgctataatt gcgcataaat aacatggtac tgtccatatg aaaaattctt
     1501 caacagagac atgaagttga ttcgtatagt aaatacctga tgttccccat ggtattaaag
     1561 gtaagaccat cgtacttgag tcttcaagtg ttcttgataa aacggaacga gagacttggt
     1621 atttttcgaa caaatctttc attaacacac cgaccataat aattacaatc gaagcaacac
     1681 ctgcagcgaa tacaagcgca atacaacaaa taacagtaat acatattaaa cttcctacag
     1741 aatggatgcc tttagaaata gtagttaata agacttctaa acatcctgct ttttctacaa
     1801 tacctgcaaa tgcatatccg caaaatatcg ttactaatat ttgggtcata ctcatcatac
     1861 caccctgttc taacaagctt ttcacactgg atgaaatatg agactgatgt atcattgatt
     1921 cgttaaaacc actaaatgtt gctttgaaac catctgtcat tttgaaatga tgattaaaag
     1981 tccccactat aatggcagaa aagcttgata ttagcatagc tggcactgta gccattttaa
     2041 atagcaaaca aacaatgatc acaattaagg gaacccagac ccatatgtta atttgataaa
     2101 tctgtgcaag ctctgataac aaagtttgaa tctgtttatc atttgaatgc cctttaaatt
     2161 gaaatccagc aataaaccat actaataaac ctatgattga tgcaggtatc gtcgtccaca
     2221 tcatcgaatg tatatgttta aatatattaa ctttagtaac aagcgccgct aaattagttg
     2281 tatctgataa tggtgacatt ttatcgccaa acacagcccc tgctataata gcacccgctg
     2341 ccatccctgg aggaatcccc aattgattac caatagaaat aagtgcaatc cctgcagttg
     2401 atgcagagcc ccatgctgta ccagttgcta cagatgtaac agcacttata aaaaaggctg
     2461 atattaaaaa atagcttgga ttcaataaat ctaagccata ataaatcaag gctggcactg
     2521 tgccagaaaa catccaactg cctactataa ttcctaccgc tagtataatt aaaattgcag
     2581 gcattgcagt atttaaacgt tctgcaatgc ctttttctaa atcttgccat gttaagccta
     2641 cacgttttgc aatccatgtg gcatatgctg aggcaataat taataatact tgaattggaa
     2701 tatcaaaaaa cacaaagcct gttacaacaa caataaccat tacaatcatt gtcgaaattg
     2761 attctaaaaa ggtgggtttt cttttcatca tctatcgtcc ccttcgctta ttgatgagaa
     2821 acctcttttc ttcaatttat tgcttttgca taatgctata tccttttcta tcttcaagcc
     2881 atcatttatt ttaaatattt ttctaaatca agcttatcta aaccaatttc ttcaactgtt
     2941 aagccttcct caaagaagtc tctctctaaa atggttgatg caattacaat tactgcatct
     3001 atatttggtg tcggtacatc aataacacga cctaagcttg accatagtac taaaccatat
     3061 gcaatatctt cagttaaata tctgctttct acatgatttg gtcctggaat ttgtgaaaat
     3121 actgggcttg tattaaacaa acgatttaat ggttcatctt ctttatcacg ttctaaataa
     3181 ccacgttcaa tacgtgattc tttagctgtt gataattcaa aacctaatct acggcctaaa
     3241 ttcaaacgtt ctaattcgat tgcatgaagt aatctaactg tatgtttagt aattccttct
     3301 ttatataaag cgaactcgcc agcataatca atgcgaccga cattcaataa tgttggtcct
     3361 ggatgcactt caggattacc attttcaaga tttgttttaa ttaagctttc ctcttttact
     3421 aaatgatcat aaatacttga aactttgtca taacaatcat ttagacagct tctatcatat
     3481 gttgaaaaga agatacgacg tacatttaga gataaatcaa ctgctgcatt ttcaaaatcg
     3541 acacgcgtac catacgtcaa cgtattagct tccgctagtt gtggttttgt ttcaatatgt
     3601 ctatcttcta aaacattcat aaaacgaatt gaccccattg ctgcagccat gttgaagaat
     3661 atcaactgat tatcagttac atgctctgcc attacatcag cataatactc tatgtatgaa
     3721 gatggaataa tcacttgaac aatttcagca tcttttaaaa catattccat atcatcacta
     3781 atatcagtga attttacgaa acgttcatca ccttcattat taaaatcaaa tccgcccttt
     3841 tcgattgcgt tttgaaactt acttatagat tgattacgac aatataattt aacatcgtgg
     3901 cctttgctca ccatatctac tgctgccgta actgcgccat ttcctgatcc tacaattgct
     3961 attttcataa acaaccctct ttctatcttt ggttaaaatt aaaaacaaat tatacgtaag
     4021 acaatataaa aatgatgcga attcatta
//
LOCUS       DAKTIO010000201         3978 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.206, whole genome shotgun sequence.
ACCESSION   DAKTIO010000201 DAKTIO010000000
VERSION     DAKTIO010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.206"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            23..637
                     /locus_tag="O0293_002478"
     CDS             23..637
                     /locus_tag="O0293_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HCY0281337.1"
                     /translation="MNTENNKNQNQSVKNSERRGMLKGCGGCLISFILLIILLSACSM
                     MFSNNDNSTNNQSSKTQLTQKDENKNEDKPEEKSETATDEDLQSTEEVPANENTENNQ
                     HEIDEITTKDQSDDDINTPNVAEDKSQDDLKDDLKEKQQSSNHHQSTQPKTSPSTETN
                     TQQSFANCKQLRQVYPNGVTADHPAYRPHLDRDKDKRACEPDKY"
     gene            718..1713
                     /locus_tag="O0293_002479"
     CDS             718..1713
                     /locus_tag="O0293_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCY0281338.1"
                     /translation="MSNQFKSEEERRQWEQFQAFQNQQNQQNQQYGQKKSKKGWFWGC
                     GGCLVLFILIIIGISACTAGITGNLGGNSSKETNKTHKIGETVKNGDLEVTVNSVETM
                     KSVGPSLAPTNAKGIFVVADVTIKNKGKEALTIDSSMFKLKSGDKTFEADNTGSMSAN
                     QSDNGSIENSFFLQRINPDSTAQGKIVFDVSENIANAKDKKLEVISSLFSVKKITFDL
                     SDAKKTSKAKKDKQDTEVAVASSNSDNVSYEASATTPATTSSADTDSEDSEKSSKDED
                     KQNASKSDKSSVEKSESNEETAPVEPMPHSKPTTSEAPPSQNIHNEDSMYDASTE"
     gene            1789..2415
                     /locus_tag="O0293_002480"
                     /pseudo
     CDS             1789..2415
                     /locus_tag="O0293_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500421.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2456..2800
                     /locus_tag="O0293_002481"
     CDS             2456..2800
                     /locus_tag="O0293_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3969 family protein"
                     /protein_id="HCY0281339.1"
                     /translation="MITFENIQQLEKYTLMTMHGLFNQLKLGIISIENAEHTLFTPYM
                     LETLSSLGVKDSIVDLIHKGTELEDFAAFNLSIEDTVTVCLQRTEELLKQYKNVEFND
                     KILINWRIIQEK"
     gene            2898..3449
                     /locus_tag="O0293_002482"
     CDS             2898..3449
                     /locus_tag="O0293_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281340.1"
                     /translation="MEKNEYIAKYNEYSQLLDATYSQAVAYLLSKYGAVTDDYYKEKS
                     YTRFLNGEIKSISKGKYTRASEGLYCHHISEDKFQNLSDLRFISKFKYSYDVQKKENL
                     VYCDLIEHLILHAIITKESHGQFGVAGLCQMIKPTVIDWYIGEYNPKPAWMQATKARA
                     YLPGILVEKLLIKIDDMLKGIEI"
     gene            complement(3667..>3978)
                     /locus_tag="O0293_002483"
     CDS             complement(3667..>3978)
                     /locus_tag="O0293_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCY0281341.1"
                     /translation="IARELGRYLFKNTDLMRDSNDNSLKNLHEMIYESDVNQFSVDLL
                     MPKKQIEALVYNFYEVNNINLSSGLSEKERNKLLNLISTKLEVSKVAAGLRLYNLGIH
                     I"
BASE COUNT         1585 a          629 c          651 g         1113 t
ORIGIN      
        1 taaatataga ggagatttac ctttgaatac agagaacaac aagaatcaaa accaatctgt
       61 taaaaattct gaaagacgcg gcatgttaaa aggatgcggc ggttgcctta tttcttttat
      121 tttattaata atcttattat cagcctgttc aatgatgttt agtaataatg acaattccac
      181 taataatcaa tcatcaaaaa cgcaattaac tcaaaaagat gaaaataaaa atgaagataa
      241 gcctgaggaa aaatcagaaa cagcaacaga tgaggattta caatcaaccg aagaagtacc
      301 tgcaaatgaa aatactgaaa ataatcaaca tgaaattgat gaaataacaa caaaagatca
      361 atcagacgat gatattaaca caccaaacgt tgcagaagat aaatcacaag acgacttgaa
      421 agatgattta aaagaaaagc aacaatcaag taaccatcat caatccacgc aacctaagac
      481 ctcaccatca actgaaacaa acacgcaaca atcatttgct aattgtaagc aacttagaca
      541 agtatatccg aatggtgtca ctgccgatca tccagcatat cgaccacatt tagatagaga
      601 taaagataaa cgtgcatgtg aacctgataa atattaaaca acaagcgaat tgaattcaaa
      661 ttgtatttag ctttatgcac taatcacata gtaaataatg agggagattt tttaggcatg
      721 agcaatcaat tcaaaagcga agaagagcga agacaatggg aacaattcca agctttccaa
      781 aatcaacaaa accaacagaa ccagcaatac ggacaaaaga aatctaaaaa aggatggttc
      841 tggggctgtg gtggttgtct agtattattt attttaatta tcatcggtat ttcagcttgt
      901 acagctggta ttacaggtaa ccttggcgga aatagttcta aagaaacgaa caaaacccat
      961 aaaatcggtg aaactgttaa aaatggcgac cttgaagtca ctgtaaattc agtggaaact
     1021 atgaaatctg taggaccatc tcttgcacca acaaacgcta aaggtatatt tgtcgttgct
     1081 gatgtgacga ttaaaaacaa aggtaaagaa gcgttaacaa ttgatagttc aatgtttaag
     1141 ctaaaatccg gtgataaaac atttgaagca gataatacag gttcaatgtc tgctaatcaa
     1201 agtgacaatg gtagtataga aaattcattt ttcttacagc gtataaatcc agatagcact
     1261 gctcaaggta aaattgtttt cgatgtgtca gaaaacatag ccaacgcaaa agataaaaaa
     1321 ttagaagtta tttctagttt atttagcgtc aagaagatta catttgattt atccgatgct
     1381 aaaaaaacat caaaagctaa aaaagacaag caagatacag aagtagctgt tgcgagttca
     1441 aatagcgata atgtaagtta tgaagcttcg gctactacac ctgctacaac ttctagtgcg
     1501 gatactgatt ctgaagatag cgaaaagtct agtaaagatg aggataagca gaatgcgtct
     1561 aaaagtgata aatctagtgt agaaaaaagt gaatctaatg aggaaactgc tcctgtagag
     1621 cccatgcccc atagcaaacc taccactagt gaagcaccac ctagccaaaa tattcacaat
     1681 gaagatagca tgtacgacgc ttcaacagaa taaaatttca gggtagctcg gctacccttc
     1741 ttttacggaa aaattaatta tacataatca aatcaaggag ataaaaaaat gaaattcaaa
     1801 gctatcgttg caatcacatt atcattgtca ctattaactg cctgtggtgc taatcaacat
     1861 aaagaaaata gtagtaaatc aaatgacact aataaaaaga cgcaacaaac tgacaacact
     1921 acacagtcaa atacagaaaa gtaaatgaca ccacaagaag ccgaagatat agttcgaaac
     1981 gattacaaag caagaggcgt taatgaatat caaacattaa attataaaac aaatcttgaa
     2041 cgaagcaatg aacatgaata ttatgttgaa catctagtcc gcgatgcagt tggcacacca
     2101 ttaaaacgtt gtgctattgt taatcgacac aatggcacaa ttattaatat ttttgatgat
     2161 atgtcagaaa aagacaaaga agaatttgaa gcatttaaaa agagaagccc taaatacaat
     2221 ccaggtatga acaatcatga tgaaacagat ggtgagtcag aagacattca acatcatgac
     2281 attgataata acaaagcaat tcaaaatgac ataccagatc aaaaagtcga cgataaaaat
     2341 gataaaaatg ctgttaataa agaagaaaaa catgataatg gggcaaataa ttctgaagaa
     2401 actaaagtta aataatggca tactttgatt aatcgtaatt tttaggagaa acgctatgat
     2461 aacatttgag aatatacaac aacttgagaa atacacctta atgactatgc atggtctttt
     2521 taatcaactt aaactcggca ttatttcaat cgaaaacgca gagcatacgc tttttacacc
     2581 ttatatgttg gaaacgctct cttccctagg cgtgaaagac agcattgtcg atttaattca
     2641 taaagggact gaattagaag actttgcggc atttaattta tcaattgaag acacagttac
     2701 agtctgttta caaagaactg aagaactatt aaaacaatac aaaaatgtgg aattcaatga
     2761 caaaatatta atcaattggc gtattataca agagaaatag acatataaaa gtcgaatgta
     2821 actacgtgag tattgatttt attctttgta aattacaagc atttcatatt ataaagtttg
     2881 aaaagaggta tattgaaatg gagaaaaatg aatatatagc taaatataat gaatatagtc
     2941 aattattaga cgctacatac tcgcaagctg tagcatacct tttaagtaaa tatggcgctg
     3001 taaccgatga ttattataaa gaaaaatcat acacgcgatt tttaaatgga gaaatcaaaa
     3061 gtatttcaaa aggaaaatac actagagcta gcgaaggatt atattgccat catataagcg
     3121 aggacaaatt ccaaaatcta tctgatctaa gattcatttc caaatttaag tactcatacg
     3181 acgttcaaaa gaaagaaaac ttagtgtact gtgatctaat cgagcattta attttacatg
     3241 caattattac aaaagaatcc catggccaat ttggtgtagc tggattatgt caaatgatca
     3301 aaccaacagt cattgattgg tacattggcg aatataatcc aaaaccagca tggatgcaag
     3361 ccaccaaagc acgtgcctat ttgcctggaa tattagtaga gaaattactc attaaaattg
     3421 acgatatgtt aaaaggaata gaaatataag atttccttga gtctagataa atgattaatg
     3481 tagatttatt ttttgctgtt gagattttgt tatagatgtt taaacctgta attaaatata
     3541 ttttataaaa tagaccacgc atacctatct ataaacggac aatgtttata aatgagtttg
     3601 catggtcttg aattgtatta aatttctttt ggttttaata aatcgactag attttcacaa
     3661 tatttatcaa atatgtattc ctaaattata cagccttaat ccagcagcta ctttcgaaac
     3721 ttccaactta gttgatataa ggttcaatag tttgtttcgt tctttttcag ataaaccaga
     3781 acttaaattg atattattga cttcataaaa attatagact aatgcctcta tttgcttttt
     3841 aggcataagt aagtcgactg aaaactgatt tacgtcgctt tcataaatca tttcatgtaa
     3901 attctttaga ctattatcgt tactatctct cattaagtct gtatttttaa ataaataacg
     3961 gcccaattca cgagctat
//
LOCUS       DAKTIO010000202         3970 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.207, whole genome shotgun sequence.
ACCESSION   DAKTIO010000202 DAKTIO010000000
VERSION     DAKTIO010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.207"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(474..2120)
                     /locus_tag="O0293_002484"
     CDS             complement(474..2120)
                     /locus_tag="O0293_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCY0281342.1"
                     /translation="MNSSSPENPNGKKYSPVFIYSAIVVAIVVLLGAFLPEQFNYVTN
                     NIKMWITEKLGWYYLILTTIIVFFCIFLIFSPIGKLKLGKPNDKPEFNTISWFAMLFS
                     AGMGIGLVFYGAAEPMAHFATPPTADPKTTEAYTEALRSTFFHWGFHAWAVYGVVALA
                     LAYSQFRKGEPGLLSRTLRPLLGDKVEGPIGIFIDVLSVFATIVGVAVSLGMGALQIN
                     GGLHYLFNVPNNTFVQAIIIIVVTILFIASAWSGLSKGIQYLSNLNIGLGTILMVAAL
                     IVGPTVLILNMLTSSTGSLLNTFLFNSFDTAALNPQKREWMSSWTLYYWGWWLSWSPF
                     VGVFIARVSKGRSIREFISGVLLVPAIVSFVWFSVFGVLGIETGKKHKEIFDMTPETQ
                     LFGVFNHVPFGIVLSLIALLLIASFFITSADSATFVLGMQTTFGSLNPSSMVKVVWGI
                     SQALIAFVLLLAGGGNGAEALNAIQSAAIISAFPFSFVVILMMVSFYKDANQERKFLG
                     LTLTPNKHRLQEYIKSQQEDYESDILEKRQSRRNIEKKDN"
     gene            2324..2686
                     /gene="mscL"
                     /locus_tag="O0293_002485"
     CDS             2324..2686
                     /gene="mscL"
                     /locus_tag="O0293_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCY0281343.1"
                     /translation="MLKEFKEFALKGNVLDLAIAVVMGAAFNKIISSLVENIIMPLIG
                     KIFGSVDFAKEWSFWGIKYGLFIQSVIDFIIIAFALFIFVKIANTLMKKEEAEEEAVV
                     EENVVLLTEIRDLLREKK"
     gene            complement(2757..3626)
                     /locus_tag="O0293_002486"
     CDS             complement(2757..3626)
                     /locus_tag="O0293_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCY0281344.1"
                     /translation="MEMSRLNLTDDNDIDEIIAWRGEQEELEQKRDTYKKRYHEFEME
                     IARLESLTKDKELLDSDKLKDEYELKKGKMNTLIDEYSAVHYQCQNNINKTQSIVSHI
                     NYLNQELKDQQEIFQLAEIVSGKNNKNLTLENFVLIYYLDQIIAQANLRLATMSDNRY
                     QLIRREAVSHGLSGLEIDVFDLHSNKSRHISSLSGGETFQSSLALALGLSEIVQQQSG
                     GISLESIFIDEGFGTLDQETLETALDTLLNLKSTGRMVGIISHVSELKNRIPLVLEVK
                     SDQYQSSTRFKRN"
BASE COUNT         1314 a          714 c          575 g         1367 t
ORIGIN      
        1 cacattattg taagctgact tttcgtcagc ttctgtgttg gggccctcac cccaactcgc
       61 attgcctgta gaatttcttt tcgaaattct ctgtgttggg gcccctgact agaattgaaa
      121 aaagcttgtt acaagcgcat tttcgttcag tcaactactg ccaatataac ttcgtagagc
      181 atagaatatt gatttatgtc ccagcctgag ttaattttct ataaaagtat atttaatttg
      241 cgtttatacc gtcaaacttc actttagctt tgtcaaaccc ctttctatta agttttcaga
      301 aataaaccta tcttaaaata taaaaaaatc gagaattcgt agtttaataa cgaaattctc
      361 gttcttatcc ttttgaatat actcaatttt ccacaaaaac aaacaagtag tatatctgtt
      421 ctagctacta gaatgacata ctacttgtta ttaaaatact taactaaact ttattagtta
      481 tcttttttct ctatatttct acgtgactga cgcttttcaa gaatgtcaga ttcataatct
      541 tcttgttgac tcttgatata ttcttgtaag cgatgtttat tcggagtcaa tgttaaacct
      601 aggaatttac gttcctggtt cgcatccttg tagaaactta ccatcatgag tatgacgaca
      661 aaggagaatg ggaatgcact tataattgca gcactttgaa tcgcatttaa agcttcagcg
      721 ccgttaccgc caccagctaa taaaagtaca aatgctatta aggcctgtga aattccccaa
      781 acaactttta ccatactaga tggatttaat gaaccaaatg ttgtttgcat tcctaataca
      841 aatgttgctg agtcagcaga tgtaataaag aatgatgcaa ttaataataa tgcaatcaac
      901 gataaaacaa tgccaaatgg cacatgatta aacactccaa atagctgtgt ttcaggagtc
      961 atatcaaaaa tttctttgtg tttcttacct gtctcgatgc ctaatacacc aaagacacta
     1021 aaccaaacaa aactaacaat tgctggaact agcaagacac cagaaatgaa ctctctaatt
     1081 gaacgtcctt ttgaaactcg tgcaataaac actccaacga atggactcca acttaaccac
     1141 caaccccagt aataaagtgt ccatgaagac atccattcac gtttttgagg atttaaagct
     1201 gctgtatcaa aactattaaa caagaatgtg tttagtaaac tacccgtaga gctagttaac
     1261 atatttaaaa taagaacagt tggtccaaca attaaagcag ctaccattaa aatagtacct
     1321 aaaccaatgt tcaagttact taagtattga atacctttac ttaatccaga ccatgcactt
     1381 gctataaata agatagtaac aacaatgatg ataatcgctt gtacaaacgt attgtttgga
     1441 acattgaaca agtaatgtaa accaccatta atttgtagag cacccatacc taacgaaacg
     1501 gctaccccaa cgattgtcgc aaatacagat aaaacgtcaa taaaaatccc aataggacct
     1561 tctactttat cacctaaaag aggacgtaaa gttctagata ataaacctgg ttcaccttta
     1621 cggaattgcg aatatgccaa cgctaacgca acaacaccat aaacagccca agcatggaat
     1681 ccccaatgga aaaatgttga acgtagagct tcagtataag cttcagtagt tttgggatct
     1741 gctgtaggtg gcgtagcaaa gtgcgccatc ggttcagctg caccataaaa caccaaacct
     1801 atccccatac cagcactaaa caacatagca aaccatgaaa ttgtattaaa ctcaggtttg
     1861 tcatttggtt tacctagttt aagttttcca ataggactaa aaataaggaa tatacagaag
     1921 aacacgataa tcgtagtaag aataagataa taccaaccta acttttctgt aatccacatt
     1981 ttaatattat tggtaacata gttgaattgt tcaggtaaaa atgcaccaag taatacgact
     2041 atagcaacaa caattgcact atagatgaag actggtgaat acttctttcc atttggattc
     2101 tctggtgaag aagaattcat aattaattac tcccttcaat tctatattta attttatgta
     2161 gtagaataaa aatattatct aaacatttta ttcaataact cacgcacctt tataggataa
     2221 caaaggtctt tgcgataatc aaattttctt gaataatcta ttttctaatt tttttatttg
     2281 ttgtataatt gaggttacat ttaaaaaaga gaggttgagt gtaatgttaa aagaattcaa
     2341 agagttcgcc ttaaaaggta acgtcttaga tttagcaatt gctgttgtga tgggtgcagc
     2401 tttcaacaag attatatctt cattagtaga aaatatcatt atgccattaa ttggtaaaat
     2461 tttcggatca gttgattttg ctaaagaatg gtcattctgg ggtattaaat acggtttatt
     2521 tatccaatct gttatcgact ttattatcat cgcgtttgct ttattcatct ttgttaagat
     2581 tgcaaataca ttaatgaaga aagaagaagc cgaagaagaa gcagttgtgg aagaaaatgt
     2641 tgtgttatta actgaaatca gagatttatt acgtgagaaa aaataatttt taaataaaaa
     2701 gagatgggct aaggttgttc tatacattcc aaagtaccat ctctattttt tatgttttaa
     2761 tttcttttga atcttgttga actctgatat tgatctgatt tcacttctaa aactaaaggt
     2821 attctatttt tcaattcgct cacatgtgaa ataatcccaa ccattctacc agttgattta
     2881 agatttaata aagtgtctaa cgctgtttca agcgtttctt gatctaatgt accgaatcct
     2941 tcatcaataa atattgattc tagtgaaata cctcctgatt gctgctgtac aatttcgctt
     3001 aaccctaaag ctaatgcaag cgacgattgg aaagtttctc cacctgataa cgagctaata
     3061 tgtctagact tatttgaatg caaatcaaat acatcaattt ctaggccact aagaccatga
     3121 gaaaccgctt ctcgcctaat tagttggtat cgattatctg acattgttgc taatctcaga
     3181 tttgcttgag caataatttg atctaaatag taaattaaga caaagttttc caatgtaaga
     3241 tttttgttat tcttaccact gacaatttca gccaattgaa atatttcttg ttgatccttt
     3301 aattcttgat ttaagtaatt aatatgcgaa actatagatt gtgttttatt aatattattt
     3361 tgacattgat aatgaacagc agagtattca tctatcagtg tattcatctt tccttttttt
     3421 agctcatatt catcttttaa tttatcagag tccaataact ctttatcctt agttaatgat
     3481 tctaacctag ctatttccat ttcaaattca tgataacgtt ttttataagt atcccttttc
     3541 tgctctaatt cctcttgctc gcccctccag gctattattt catctatatc attgtcatca
     3601 gttaaattca gcctcgacat ttccatttca atagactgtt cattcaattc taagtcatta
     3661 ttgtaagttt ctagttgatt ttcataatga tttagattat tttgctcaat taactttctt
     3721 tgagacaatt gcattaactt atcttcaatc tcctttgaga gttcttgatg ctgattcaca
     3781 tcttgaacat acttcttata atccacctca aaattagtga tattattata tttcgagagt
     3841 gtttcaaatt cagtgatgag atctcgacat aatttcaact cagattcatt tttatttaga
     3901 cgcaattgtt tattatgaat ttgcaacgtt agattatctt tttcttcttt catttgttct
     3961 ataaatttat
//
LOCUS       DAKTIO010000203         3923 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.208, whole genome shotgun sequence.
ACCESSION   DAKTIO010000203 DAKTIO010000000
VERSION     DAKTIO010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.208"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(105..659)
                     /locus_tag="O0293_002487"
     CDS             complement(105..659)
                     /locus_tag="O0293_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HCY0281345.1"
                     /translation="MIQDAFVALDFETANGKRTSICSVGMVKVIDSQITETFHTLVNP
                     QDYFSQQNIKIHGIQPEDVENAPTFDYVFPYMMQFIADLPVVAHNAAFDMNVLHQSIQ
                     NIGLPTPNLTYFCSYQLAKRTVDSYRYGLKHMMEFYQLDFHGHHDALNDAKACAMITF
                     RLLKNYENLTYVTNIYGKNLKDKG"
     gene            874..966
                     /locus_tag="O0293_002488"
     CDS             874..966
                     /locus_tag="O0293_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281346.1"
                     /translation="MTKNTIISLENEKTQINDSENESSDLRKAK"
     gene            complement(1048..1548)
                     /gene="ftnA"
                     /locus_tag="O0293_002489"
     CDS             complement(1048..1548)
                     /gene="ftnA"
                     /locus_tag="O0293_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-type ferritin FtnA"
                     /protein_id="HCY0281347.1"
                     /translation="MLSKNLLEALNDQMNHEYFAAHAYMAMAAYCDKESYEGFANFFI
                     QQAKEERFHGQKIYNYINDRGAHAEFRAVSAPKIDFSSILETFKDSLSQEQEVTRRFY
                     NLSEIARQDKDYATISFLNWFLDEQVEEESMFETHINYLTRIGDDSNALYLYEKELGA
                     RTFDEE"
     gene            1840..3153
                     /gene="murT"
                     /locus_tag="O0293_002490"
     CDS             1840..3153
                     /gene="murT"
                     /locus_tag="O0293_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HCY0281348.1"
                     /translation="MRQWTAIHLAKLARKASRAVGKRGTDLPGQIARKVDTDILRKLA
                     EQVDDIVFISGTNGKTTTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKT
                     KIAVIEIDEGSIPRVLKEVTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKL
                     LLNADDPFVSRLKIASDTIVYYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQ
                     IGHYHCQCGFKREQAKYEISSFDVAPFLYLNINDEKYDMKIAGDFNAYNALAAYTVLR
                     ELGLNEQTIKNGFETYTSDNGRMQYFKKERKEAMINLAKNPAGMNASLSVGEQLEGEK
                     VYVISLNDNAADGRDTSWIYDADFEKLSKQQIEAIIVTGTRAEELQLRLKLAEVEVPI
                     IVERDIYKATAKTMDYKGFTVAIPNYTSLAPMLEQLNRSFEGGQS"
     gene            3155..3886
                     /locus_tag="O0293_002491"
     CDS             3155..3886
                     /locus_tag="O0293_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCY0281349.1"
                     /translation="MHELTIYHFMSDKLNLYSDIGNIIALRQRAKKRNIKVNVVEINE
                     TEGITFDECDIFFIGGGSDREQALATKELSKIKTPLKEAIEDGMPGLTICGGYQFLGK
                     KYITPDGTELEGLGILDFYTESKTNRLTGDIVIESDTFGTIVGFENHGGRTYHDFGTL
                     GHVTFGYGNNDEDKKEGIHYKNLLGTYLHGPILPKNYEITDYLLEKACERKGIPFEPK
                     EIDNEAEIQAKQVLIDRANRQKKSR"
BASE COUNT         1407 a          535 c          697 g         1284 t
ORIGIN      
        1 aacttataaa aatatgacga tatatgactt tatataaaat aaagctccct gcaaagttta
       61 catttttaca atgcttactt ttgaagggag tattttattt agtcctagcc tttatctttt
      121 agatttttac cataaatatt agttacatat gttaaatttt cataattttt cagtagccta
      181 aaagtaatca ttgcgcatgc tttggcatca ttcaatgcat catgatgacc atgaaaatct
      241 aattgataaa actccatcat atgttttaaa ccgtatcgat acgaatcaac ggttctttta
      301 gcaagttgat aactacaaaa gtaagttaaa tttggagttg gtaaaccaat attttgaatg
      361 ctttgatgta agacgttcat atcaaatgcc gcgttatgtg cgacaacagg taaatctgca
      421 ataaattgca tcatatatgg aaatacgtag tcaaacgtag gtgcattttc aacatcttct
      481 ggttgtatgc catgaatttt aatattttgt tgtgaaaaat agtcttgcgg attcacaaga
      541 gtatgaaatg tttctgttat ttgactatca atgactttaa ccattccgac agaacaaata
      601 cttgtacgtt taccatttgc tgtttcaaaa tcaagtgcaa caaacgcatc ttgtatcatg
      661 tagtcaatcc tttcaattaa ttaaatgtat atattaatag acttttagtc atttgcttac
      721 aaacgataca taatattata cgtgaaggta taacgtgaaa tctatcattt ggatgtaact
      781 ctgattaaaa agtaaatgat gatatgcttt aaaatcgtca aattaattca acatcaaatt
      841 tagagttatt aacattagta gtttaaaaaa taaatgacta aaaatacaat aattagcctt
      901 gaaaatgaaa aaacgcagat caatgattca gaaaatgaat ctagtgatct ccgtaaagca
      961 aaataatata gacattgggt tattgtaagt gacccaaatg cctatcatgt cgtatgatat
     1021 ttatctgtct attgtagtga tgtttaatta ttcttcgtcg aatgtacgag cgccaagttc
     1081 tttttcgtaa agatataatg cattgctgtc atcgccgata cgagttaaat aattgatgtg
     1141 agtttcaaac attgattctt cttcgacttg ttcatctaag aaccagttta agaatgagat
     1201 agttgcataa tctttatctt gacgagcgat ttcagataag ttatagaaac gtcttgttac
     1261 ttcttgttct tgagataagc tgtctttgaa agtttctagt atgcttgaaa agtcaatttt
     1321 tggtgctgaa actgctctga attctgcatg tgcacctctg tcgttaatat agttataaat
     1381 cttttgtcca tggaaacgtt cttctttagc ttgttgaatg aagaagtttg caaatccttc
     1441 gtacgattct ttatcacagt atgctgccat tgccatatat gcgtgtgctg caaagtactc
     1501 atggttcatt tgatcattta atgcttctaa taaattttta cttaacattt tgatacacct
     1561 cttatttgta aattaaaatt actatgaatt aaacttagta taacaaactc attctcaatt
     1621 gtaaataata ataattataa tttaatgcct tattttagca ctctaattga gaaaatgtat
     1681 catttagcga tatttaattg agaataaata tcatttttaa ttatctaaat gagaatgatt
     1741 ataatttaga aacactgtat caatttgtga ttgagaaact tttttgaagt tgccttgtca
     1801 tgttataatg atgcgagtgg aaatttgagg aggaaacaca tgagacagtg gacggcaatc
     1861 catctagcga aattggcgcg taaagcaagt agagcagtag gtaaaagagg aacagattta
     1921 cctggacaaa tcgctagaaa agtggataca gatatattaa gaaaattagc agagcaagtt
     1981 gatgatattg tatttatcag tggaacaaat ggtaaaacaa cgacttcaaa cttaattgga
     2041 catactttaa aagcaaataa tattcaaatt atacacaata atgaaggtgc taatatggct
     2101 gcaggtataa cttctgcatt catcatgcaa tcaacaccta agactaaaat tgcggtaatc
     2161 gaaattgatg aaggttcgat tccacgtgtg ttaaaagaag ttacaccttc aatgatggta
     2221 tttactaatt tctttagaga tcaaatggat cgcttcggtg aaattgatat tatggttaat
     2281 aacattgcag agacaattag taataaaggc atcaaattat tgctaaatgc tgatgatcca
     2341 tttgtgagtc gtttgaaaat cgcaagtgat acgattgtgt actatggtat gaaagcacat
     2401 gcccatgaat ttgaacaaag tacgatgaat gaaagtagat attgtccaaa ctgtggtcgc
     2461 ttattgcaat acgattatat tcattataat caaattggtc attatcactg tcagtgtggt
     2521 ttcaaacgag agcaagcaaa atatgaaata tcaagttttg atgtggcacc gtttttatat
     2581 ttaaatatca atgatgaaaa atatgatatg aaaattgcag gtgactttaa cgcttataac
     2641 gcgttagcag catatactgt tttaagagag ctagggttaa atgaacaaac aattaaaaat
     2701 ggctttgaaa cgtatacatc agacaatggt cgtatgcagt actttaaaaa agaacgaaaa
     2761 gaagcgatga tcaatttagc taaaaatcct gcaggaatga atgcaagttt atcagttggt
     2821 gaacaattag aaggcgaaaa agtgtatgtt atttcgctaa atgataacgc tgcagatggt
     2881 cgagatactt catggattta tgatgcagat tttgaaaaat tatctaagca acaaattgaa
     2941 gctatcatcg tgacaggtac acgagcagaa gaacttcaat tgcgattgaa gttagcagag
     3001 gttgaagtac caattatagt tgagcgtgat atttataaag caacggcaaa gactatggat
     3061 tataaaggtt tcacagttgc aataccaaac tatacatcat tagcgcctat gcttgaacaa
     3121 ttaaaccgtt cgtttgaagg aggtcaatca taatatgcat gaattgacta tttatcattt
     3181 tatgtcagat aaattgaatt tatacagtga tataggaaat attattgctt taagacaacg
     3241 tgctaaaaaa cgaaatatta aagttaatgt cgtagaaatc aatgaaacag aaggtattac
     3301 ctttgatgaa tgtgatattt tctttatcgg tggtggaagt gatagagaac aagcattagc
     3361 aacaaaagaa ttaagtaaaa ttaagacacc acttaaagaa gcgattgaag atggtatgcc
     3421 gggattaacg atttgtggag gctatcaatt tttagggaaa aaatatatca cgcctgatgg
     3481 tacagaatta gaagggttag gtattttaga tttttatact gaatcaaaga caaaccgatt
     3541 aacaggagat attgttatcg aaagtgatac ttttggaact attgtaggtt ttgaaaatca
     3601 cggtggtaga acatatcatg atttcggtac acttggtcat gttacttttg gttatggtaa
     3661 taatgatgaa gataaaaaag aaggcattca ttataaaaat ttattaggta cttatttaca
     3721 tggaccaatt ttacctaaaa attacgaaat cactgattat ctgttagaaa aagcttgtga
     3781 acgtaagggt attccgtttg agcctaaaga aatagataat gaagcggaaa tacaagcgaa
     3841 acaagtatta atagacagag caaatagaca gaagaaatct cgttaactct gaacatcgca
     3901 tcaatggatt taatattgat aaa
//
LOCUS       DAKTIO010000204         3888 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.209, whole genome shotgun sequence.
ACCESSION   DAKTIO010000204 DAKTIO010000000
VERSION     DAKTIO010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.209"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(12..533)
                     /locus_tag="O0293_002492"
     CDS             complement(12..533)
                     /locus_tag="O0293_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2529 domain-containing protein"
                     /protein_id="HCY0281350.1"
                     /translation="MSKILNTQLIGIFNRLEKQSLEIQMAAQCLIQAIGGEGYVYVKG
                     YDDLQFFESFILHSDERLKSSRKLDAIKDFKEIDSTDRVLLFAPFYNDQVALDIQKLI
                     DLDIDVVLISNKPKTDDFPDHLVHFIDLSTPRPIVYTEDYDKIVQPHAMALNYVYYDI
                     YTQMIEMTRDLEL"
     gene            751..1611
                     /locus_tag="O0293_002493"
     CDS             751..1611
                     /locus_tag="O0293_002493"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002468309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HCY0281351.1"
                     /translation="MPLVSMKEMLIDAKENGYAVGQYNINNLEFTQAILEASQEENAP
                     VILGVSEGAARYMSGFYTIVKMVEGLMHDLNITIPVAIHLDHGSSFEKCKEAIDAGFT
                     SVMIDASHSPFEENVATTKKVVEYAHEKGVSVEAELGTVGGQEDDVVADGIIYADPKE
                     CQELVEKTGIDALAPALGSVHGPYKGEPKLGFKEMEEIGLSTGLPLVLHGGTGIPTKD
                     IQKAIPFGTAKINVNTENQIASAKAVRDVLNNDKEVYDPRKYLGPAREAIKETVKGKI
                     KEFGTSNRAK"
     gene            2077..3336
                     /locus_tag="O0293_002494"
     CDS             2077..3336
                     /locus_tag="O0293_002494"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCY0281352.1"
                     /translation="MAQEVIKIRGGRTLNGEVNISGAKNSAVAIIPATLLAQGHVKLE
                     GLPQISDVKTLVSLLEDLNIKASLNGTELEVDTTEIQNAALPNNKVESLRASYYMMGA
                     MLGRFKKCVIGLPGGCPLGPRPIDQHIKGFKALGAEIDESSTTSMKIEAKELKGAHIF
                     LDMVSVGATINIMLAAVYATGQTVIENAAKEPEVVDVANFLTSMGANIKGAGTSTIKI
                     NGVKELHGSEYQVIPDRIEAGTYMCIAAACGENVILNNIVPKHVETLTAKFSELGVNV
                     DVRDERIRINNNAPYQFVDIKTLVYPGFATDLQQPITPLLFMANGPSFVTDTIYPERF
                     KHVEELKRMGANIEVDEGTATIKPSTLHGAEVYASDLRAGACLIIAGLIAEGVTTIYN
                     VKHIYRGYTDIVEHLKALGADIWTETV"
     gene            3425..3760
                     /locus_tag="O0293_002495"
     CDS             3425..3760
                     /locus_tag="O0293_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0281353.1"
                     /translation="MEVCPYLEETFKILGRSWNGLIINYLSRCNDCSAHFSDMKRDLK
                     TITPRALSLKLSELAQWELVEKQIISTSPVQIIYVLTEKGKALAEALHPIEAWAQSYV
                     DLTDQRTAK"
BASE COUNT         1408 a          538 c          723 g         1219 t
ORIGIN      
        1 aatagtgact tttataattc taagtctctt gtcatttcaa tcatttgtgt ataaatgtca
       61 tagtatacat aattcaatgc catcgcatgt ggttggacaa tcttatcgta atcttcagtg
      121 tagactatag gtcttggtgt agataaatcg ataaaatgta cgagatgatc agggaaatca
      181 tctgttttag gtttgttgct tattaagacc acatcgatat ctaagtcgat aagtttttga
      241 atatctaatg caacttgatc attataaaat ggtgcgaata ataatacacg atcagttgag
      301 tcaatttctt taaagtcttt aatagcgtca agttttcggc tagattttaa acgctcatca
      361 ctatgtagaa tgaaactttc gaaaaactgt aaatcatcat aaccttttac ataaacataa
      421 ccttcgccac caattgcttg aattaaacat tgggcggcca tttgaatttc taaagattgt
      481 ttttctagcc tattaaagat acctattagt tgtgtgttta agatttttga catctttatc
      541 ctccaatcta cttataaaat attgtaatta atgactacat attatgcaac ggcttaaatt
      601 gtataaaaat gtatacgttt gcatttagta taactatcgc atttttcaaa aaatacacat
      661 ttaatctgca gtatttcaat gcattgacgc tatttttttg atataattac tttgaaaaat
      721 acgtgcgtaa gcactcaagg aggaactttc atgcctttag tttcaatgaa agaaatgtta
      781 attgatgcaa aagaaaatgg ttatgcggta ggtcaataca atattaataa cctagaattc
      841 actcaagcaa ttttagaagc gtcacaagaa gaaaatgcac ctgtaatttt aggtgtttct
      901 gaaggtgctg ctcgttacat gagcggtttc tacacaattg ttaaaatggt tgaagggtta
      961 atgcatgact taaacatcac tattcctgta gcaatccatt tagaccatgg ttcaagcttt
     1021 gaaaaatgta aagaagctat cgatgctggt ttcacatcag taatgatcga tgcttcacac
     1081 agcccattcg aagaaaacgt agcaacaact aaaaaagttg ttgaatacgc tcatgaaaaa
     1141 ggtgtttctg tagaagctga attaggtact gttggtggac aagaagatga tgttgtagca
     1201 gacggcatca tttatgctga tcctaaagaa tgtcaagaac tagttgaaaa aactggtatt
     1261 gatgcattag cgccagcatt aggttcagtt catggtccat acaaaggtga accaaaatta
     1321 ggatttaaag aaatggaaga aatcggttta tctacaggtt taccattagt attacacggt
     1381 ggtactggta tcccgactaa agatatccaa aaagcaattc catttggtac agctaaaatt
     1441 aacgtaaaca ctgaaaacca aatcgcttca gcaaaagcag ttcgtgacgt tttaaataac
     1501 gacaaagaag tttacgatcc tcgtaaatac ttaggacctg cacgtgaagc catcaaagaa
     1561 acagttaaag gtaaaattaa agagttcggt acttctaacc gcgctaaata attaatattt
     1621 agtctttaag ttattaataa cgtagggata ttaattttaa aagaagcaga caaaatggtg
     1681 tttgcttctt ttttatgtcg tataagtaat aaataaaaca gtttgatttt aaaatgaaag
     1741 cgtaaaaatg gtaaaatata tcaaaattga ttgtgatata attataagga aaatgagcaa
     1801 tttatgaaaa aagtttacgt gaaaaccgta gaattaaaac taaataattg tcaaaacaac
     1861 gtcaatattt agttgaataa tcagacttta gaccatggtc aagtggggaa gaacagcata
     1921 tattagtaaa ggtgaatgat ttgttattaa tcaatcgaaa atagaaagac aagattttaa
     1981 cgattaaaat aaactatttt acaaataaag taaaattaat ttataatgct aataatgcaa
     2041 aaaattaaaa agtaatggac aaaggagata aatgatatgg ctcaagaggt aataaaaata
     2101 agaggtggac gcacactaaa tggggaagtt aatatcagtg gcgcgaaaaa cagtgctgta
     2161 gctatcattc ctgcaacatt attagctcaa ggacatgtga aattagaagg gttaccgcaa
     2221 atctctgatg ttaaaacttt agtgagttta ttagaagatt taaatattaa ggcatcacta
     2281 aatggtacgg aattagaagt tgatacaact gaaatacaaa atgctgcatt accaaataat
     2341 aaagttgaat cattacgtgc ttcatactat atgatgggtg ccatgttagg tagatttaaa
     2401 aaatgtgtga ttggtttacc gggaggatgt ccacttgggc cacgtccaat tgatcaacat
     2461 attaaaggat ttaaagcttt aggtgctgaa attgatgaat caagtacgac atcaatgaaa
     2521 atcgaagcta aagagttaaa aggtgcacat atttttctag atatggttag cgtaggtgca
     2581 acaattaaca ttatgctagc tgcagtttat gcaacaggac aaactgtaat tgaaaatgct
     2641 gctaaagaac cggaagtagt tgatgttgct aacttcttaa caagtatggg agctaatatt
     2701 aagggagcgg gtacatcaac aattaaaatc aatggcgtca aagaattaca tggttctgag
     2761 tatcaagtta tacctgatag aattgaagca ggcacatata tgtgtatcgc tgcagcatgt
     2821 ggagaaaatg ttatattaaa taatattgtt ccgaaacatg tagaaacatt aactgctaaa
     2881 tttagtgaat taggtgtgaa tgttgatgta agagacgaaa gaattcgcat caataataat
     2941 gcgccatatc aatttgttga tattaaaaca ctagtatatc caggttttgc tactgatttg
     3001 caacagccta ttacaccatt attatttatg gcaaatggtc cttcatttgt aactgatacg
     3061 atttatccgg agcgttttaa acatgttgaa gaattaaagc gcatgggtgc aaatattgaa
     3121 gttgacgaag gcacagcaac gataaaacca tcaacattac atggtgctga agtttatgct
     3181 agcgatttaa gagcaggagc ttgtttaatt attgctggtt taattgctga gggtgtaact
     3241 acaatttaca atgttaaaca tatttataga ggttatacag atattgtaga acacttaaaa
     3301 gctttaggtg cagatatttg gacggaaact gtatagattt atcttgtttt gtggtataat
     3361 aattatactg attaaaaaat ggtgttcgtt tacattctaa atttattgat gagaggtgaa
     3421 gatgatggaa gtatgtccgt atctcgaaga aacttttaaa atacttggta gaagttggaa
     3481 tggattaatc attaattatc tctcaagatg taatgactgt tcagcacact tttccgatat
     3541 gaaaagagat ttgaaaacaa taacaccacg tgctttaagt cttaagttgt cagagcttgc
     3601 acaatgggag ttagttgaga agcaaatcat ttctacgagt ccagtacaaa ttatttatgt
     3661 gctaactgaa aaaggtaaag cgttagcaga ggctttacat ccaattgaag catgggcgca
     3721 atcatatgtc gatttaacag atcaacgtac tgctaaataa ttacatgacg tttaaacata
     3781 gcattgatta taactatttc taagtcttcg cattatttgc gatgatgtgg gaatagttat
     3841 ttttatttaa aaatataaaa aaatagatgc agcaaaattt taaagcat
//
LOCUS       DAKTIO010000205         3859 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.210, whole genome shotgun sequence.
ACCESSION   DAKTIO010000205 DAKTIO010000000
VERSION     DAKTIO010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.210"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            187..1107
                     /gene="mvk"
                     /locus_tag="O0293_002496"
     CDS             187..1107
                     /gene="mvk"
                     /locus_tag="O0293_002496"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HCY0281354.1"
                     /translation="MTRKGYGESTGKIILIGEHAVTFGEPAIAVPFNAGKIKVLIEAL
                     ESGNYSSIKSDVYDGMLYDAPDHLKSLVNRFVELNNITEPLAVTIQTNLPPSRGLGSS
                     AAVAVAFVRASYDFLGKSLTKEELIEKANWAEQIAHGKPSGIDTQTIVSGKPVWFQKG
                     HAETLKTLSLDGYMVVIDTGVKGSTRQAVEDVHKLCEDPQYMSHVKHIGKLVLRASDV
                     IEHHNFEALADIFNECHADLKALTVSHDKIEQLMKIGKENGAIAGKLTGAGRGGSMLL
                     LAKDLPTAKNIVKAVEKAGAAHTWIENLGG"
     gene            1112..2095
                     /gene="mvaD"
                     /locus_tag="O0293_002497"
     CDS             1112..2095
                     /gene="mvaD"
                     /locus_tag="O0293_002497"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HCY0281355.1"
                     /translation="MIKSGKARAHTNIALIKYWGKKDEALIIPMNNSISVTLEKFYTE
                     TKVTFNDQLTQDQFWLNGEKVSGKELEKISKYMDIVRNRAGIDWYAEIESDNFVPTAA
                     GLASSASAYAALAAACNQALDMQLSDKDLSRLARIGSGSASRSIYGGFAEWEKGYSDE
                     TSYAVPLESNHFEDDLAMIFVVINQHSKKVPSRYGMSLTRNTSRFYQYWLDHIDEDLA
                     EAKAAIQDKDFKRLGEVIEENGLRMHATNLGSTPPFTYLVQESYDVMALVHECREAGY
                     PCYFTMDAGPNVKILVEKKNKQQIIDKLLTQFDNNQIIDSDIIATGIEIIE"
     gene            2108..3184
                     /locus_tag="O0293_002498"
     CDS             2108..3184
                     /locus_tag="O0293_002498"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HCY0281356.1"
                     /translation="MIQVKAPGKLYIAGEYAVTEPGYKSVLIALDRFVTATIEETDQY
                     KGTIHSKALHHNPVTFSRDEDSIVISDPHAAKQLNYVVTAIEIFEQYAKSCDIAMKHF
                     HLTIDSNLDDSNGHKYGLGSSAAVLVSVIKVLNEFYDMKLSNLYIYKLAVIANMKLQS
                     LSSCGDIAVSVYSGWLAYSTFDHEWVKHQIEDTTVEEVLIKNWPGLHIEPLQAPENME
                     VLIGWTGSPASSPHFVSEVKRLKSDPSFYGDFLEDSHRCVEKLIHAFKTNNIKGVQKM
                     VRQNRTIIQRMDKEATVDIETEKLKYLCDIAEKYHGASKTSGAGGGDCGITIINKDVD
                     KEKIYDEWTKHGIKPLKFNIYHGQ"
     gene            3361..3702
                     /locus_tag="O0293_002499"
     CDS             3361..3702
                     /locus_tag="O0293_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HCY0281357.1"
                     /translation="MKNTFLICDECQAVNIRTLQKKLEKLDPDAEIVIGCQSYCGPGR
                     RKTFTFVNNRPLAALTEEELIEKVSQQLKKPRDPEEEERLRKRHEERKRRKVEQDRKL
                     KEKLEKRKAQQ"
BASE COUNT         1400 a          537 c          810 g         1112 t
ORIGIN      
        1 tttatactaa tcaagttaag tcaaatataa attcaaaata aagattatac ataatatgaa
       61 taatgtgaga taaagactgc aatgaaattt aacgagaatg cgtatgcaac ttagaatatg
      121 atgtctttta ctaataaata aaaataatga atcaagtata ggataaatat aaaaggtgat
      181 attgacatga caagaaaagg atatggggaa tcgacaggta agattatttt aataggagaa
      241 catgctgtta catttggaga gcctgctatt gcagtaccgt ttaacgcagg taaaatcaaa
      301 gttttaatag aagccttaga gagcgggaac tattcgtcta ttaaaagcga tgtttacgat
      361 ggtatgttat atgatgcgcc tgaccatctt aagtctttgg tgaaccgttt tgtagaatta
      421 aataatatta cagagccgct agcagtaacg atccaaacga atttaccacc atcacgtgga
      481 ttaggatcga gtgcagctgt cgcggttgct tttgttcgtg caagttatga ttttttaggg
      541 aaatcattaa cgaaagaaga actcattgaa aaggctaatt gggcagagca aattgcacat
      601 ggtaaaccaa gtggtattga tacgcaaacg attgtatcag gcaaaccagt ttggttccaa
      661 aaaggtcatg ctgaaacgtt gaaaacgtta agtttagacg gctatatggt tgttatagat
      721 actggtgtga aaggttcaac aagacaagca gtagaagatg ttcataaact ttgtgaggac
      781 cctcagtaca tgtcacatgt aaaacatatc ggtaagttag ttttacgtgc gagtgatgtg
      841 attgaacatc ataactttga agccttagcg gatattttta atgaatgtca tgcggattta
      901 aaggcgttga cagttagtca tgataaaata gaacaattaa tgaaaattgg taaagaaaat
      961 ggtgcgattg ctggaaaact tactggcgct ggtcgtggtg gaagtatgtt attgcttgcc
     1021 aaagatttac caacagcgaa aaatattgta aaagctgtag aaaaagctgg tgcagcacat
     1081 acttggattg agaatttagg aggttaatgc gttgattaaa agtggcaaag cacgtgcaca
     1141 tacgaatatt gcacttataa aatattgggg taaaaaagat gaagcactaa tcattccaat
     1201 gaataatagc atatctgtta cattagaaaa attttacact gaaacgaaag tcacttttaa
     1261 cgaccagtta acacaggatc aattttggtt gaatggtgaa aaggttagtg gcaaagaatt
     1321 agagaaaatt tcaaaatata tggatattgt cagaaataga gctggcatcg attggtatgc
     1381 agaaattgaa agcgacaatt ttgtaccaac agcagcaggg ttggcttcat cggcaagcgc
     1441 atatgcagct ttagcagcag cttgtaatca agcgctagac atgcagctgt cagataagga
     1501 tttatcgaga ttggcgcgaa ttggttcggg ttctgcgtcg cgtagtattt atggtggatt
     1561 tgcagaatgg gaaaaagggt atagtgatga gacgtcatat gccgttccac ttgaatcgaa
     1621 tcattttgaa gatgaccttg ccatgatatt tgttgtgatt aatcaacatt ctaaaaaggt
     1681 acctagtcga tatggtatgt cattgacacg aaacacatca aggttttatc aatattggtt
     1741 agatcatatt gatgaagatt tagctgaagc aaaagcagcg attcaagaca aagattttaa
     1801 acgccttggt gaagtaattg aagaaaatgg tttgcgtatg catgccacga atctaggatc
     1861 aacaccgccg ttcacatatc ttgtgcaaga aagttatgat gtcatggcgc ttgttcacga
     1921 atgccgagaa gcggggtatc cgtgttattt tacaatggat gcgggaccta atgtgaaaat
     1981 acttgtagaa aagaaaaaca agcaacagat tatagataaa ttattaacac agtttgataa
     2041 taaccaaatt attgatagtg acattattgc cacaggaatt gaaataattg agtaaggaag
     2101 agataaaatg attcaggtca aagcacccgg aaaactttat attgctggag aatatgctgt
     2161 aacagaacca ggatataaat ctgtacttat tgcgttagat cgttttgtaa ctgctactat
     2221 tgaagaaaca gaccaatata aaggtaccat tcattcaaaa gcattacatc ataacccagt
     2281 tacatttagt agagatgaag atagtattgt catttcagat ccacatgcag caaaacaatt
     2341 aaattatgtg gtcacagcta ttgaaatatt tgaacaatac gcgaaaagtt gcgatatagc
     2401 gatgaagcat tttcatctga ctattgatag taatttagat gattcaaatg gtcataaata
     2461 tggattaggt tcaagtgcag cagtacttgt gtcagttata aaagtattaa atgaatttta
     2521 tgatatgaag ttatctaatt tatacattta taaactagca gtgattgcaa atatgaagtt
     2581 acaaagttta agttcatgcg gagatattgc tgtgagtgta tatagtggat ggctagcgta
     2641 tagtactttt gatcatgaat gggttaagca tcaaattgaa gatactacgg ttgaagaagt
     2701 tttaatcaaa aactggcctg gattgcacat cgaaccatta caagcacctg aaaatatgga
     2761 agtacttatc ggttggactg gctcaccggc gtcatcacca cactttgtta gcgaagtgaa
     2821 acgtttgaaa tcagatcctt cattttacgg tgacttctta gaagattcac atcgttgtgt
     2881 tgaaaaactt attcatgctt ttaaaacaaa taacattaaa ggtgtgcaaa agatggtgcg
     2941 tcagaatcgt acaattattc aacgtatgga taaagaagct acagttgata tagaaactga
     3001 aaagctaaaa tatttgtgtg atattgctga aaagtatcac ggcgcatcta aaacatcagg
     3061 cgctggtggt ggagactgtg gtattacaat tatcaataaa gatgtagata aagaaaaaat
     3121 ttatgatgaa tggacaaaac atggtattaa accattaaaa tttaatattt atcatgggca
     3181 ataaatgatt tgatagggga gcaatcatct aatttaatta gaagataaat gctccatttt
     3241 ttattgtcag ccgtgttacg tatcttgaaa aaaatattaa aaagagtaaa atagatagag
     3301 ttaacgaaaa aattaatgaa atcgacgata tagatatgat aaagaaaggt gggtagcaat
     3361 atgaaaaata cattccttat ttgtgatgaa tgtcaggcag tcaatataag aacgttacaa
     3421 aagaagttgg aaaaattaga tcccgatgct gaaatcgtga taggttgtca atcttattgt
     3481 ggacctggac gccgaaaaac attcactttt gttaataacc gcccactggc tgcgcttact
     3541 gaagaagaat taatcgaaaa agtttctcaa caattaaaga aaccacgtga tcctgaagaa
     3601 gaagagcgtt taagaaaacg acatgaagaa cgtaaacgtc gtaaagtaga acaagataga
     3661 aagcttaaag aaaaattaga aaagcgaaaa gcacaacaat aaagcctgat ggcagcatca
     3721 ttcaatgcgt gccaccaggt ttttatgttt tgtctagaaa ttaaataaat cattaaatga
     3781 ttcggccatc gtaggatgcg tataaatatt atctcgtaat acggtatatg gaatgttttg
     3841 atcaatcgca agtttaatt
//
LOCUS       DAKTIO010000206         3838 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.211, whole genome shotgun sequence.
ACCESSION   DAKTIO010000206 DAKTIO010000000
VERSION     DAKTIO010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.211"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..1163
                     /locus_tag="O0293_002500"
     CDS             <1..1163
                     /locus_tag="O0293_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diglucosyl diacylglycerol synthase"
                     /protein_id="HCY0281358.1"
                     /translation="KKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHDLFMEA
                     HPILTSICKKWYINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIKEKPDL
                     ILLTFPTPVMSVLTEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQDFIDV
                     GIDPSTVKVTGIPIDNKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGFDTMIT
                     DILAKSANAQVVMICGKSKELKRSLTAKFKSNENVLILGYTKHMNEWMASSQLMITKP
                     GGITITEGFARCIPMIFLNPAPGQELENALYFEEKGFGKIADTPEEAIKIVASLTNGN
                     EQLTNMISTMEQDKIKYATQTICRDLLDLIGHSSQPQEIYGKVPLYARFFVK"
     gene            1141..2331
                     /locus_tag="O0293_002501"
     CDS             1141..2331
                     /locus_tag="O0293_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0281359.1"
                     /translation="MQDSSLNNYANHKNFILMLIILFLMEFARGMYILSYINFLPTVT
                     SIAVAITSLAFSIHFIADASTNFVIGFLLKKFGTKIVLTTGFILAFTSLFLVIWFPAS
                     PFVIIFSAMMLGIAVSPIWVIMLSSVEEDKRGKQMGYVYFSWLLGLLVGMVFMNLLIK
                     VHPTRFAFMMSLVVLIAWILYYFVDVKLTNYNTRPVKAQLRQIVDVTKRHLLLFPGIL
                     LQGAAIAALVPILPTYATKVINVSTIEYTVAIIIGGIGCAVSMLFLSKLIDNRSRNFM
                     YGVILSGFILYMILIFTLSMIVNIHILWIIALAIGLMYGILLPAWNTFMARFIKSDEQ
                     EETWGVFNSIQGFGSMIGPLFGGLITQFTNNLNNTFYFSALIFLVLAVFYGSYFIVNR
                     EKAK"
     gene            complement(2444..2953)
                     /locus_tag="O0293_002502"
     CDS             complement(2444..2953)
                     /locus_tag="O0293_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcG family protein"
                     /protein_id="HCY0281360.1"
                     /translation="MILGLALIPSKSFQEAVDSYRKRYDKQYSRIKPHVTIKAPFEIK
                     DGDLDSVIEQVRARINGIPAVEVHATKASSFKPTNNVIYFKVAKTDDLEELFNRFNGE
                     DFYGEAEHVFVPHFTIAQGLSSQEFEDIFGQVALAGVDHKEIIDELTLLRFDDDEDKW
                     KVIETFKLA"
     gene            complement(3147..>3838)
                     /locus_tag="O0293_002503"
     CDS             complement(3147..>3838)
                     /locus_tag="O0293_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCY0281361.1"
                     /translation="TLSIYLPESYNQLVKYNVILCFDGLDFLRFGRIQRTYESLIKEA
                     RIDDAIIVGFHYEDVDKRREEFHPQGSRSHLTIQSVGKEILPFIDSTFSTLKVGNARL
                     LVGDSLAGSIALLTALTYPTIFSRVAMLSPHSDEKVLDKLNQCANKEQLTIWHVIGLD
                     EKDFTLPTNGKRADFLTPNRELAEQIKKYNITYYYDEFDGGHQWKDWKPLLSDILLYF
                     LSKNTDDQLYE"
BASE COUNT         1275 a          635 c          587 g         1341 t
ORIGIN      
        1 ataaaaagat attgattatt actggctcat tcggtaacgg tcatatgcaa gttacacaga
       61 gtatcgttaa tcaacttaat gatatgaatc tagaccattt aagcgtcatt gagcacgatt
      121 tatttatgga agctcatcca attttgactt ctatttgtaa aaaatggtat atcaatagct
      181 ttaaatattt tagaaatatg tacaaagggt tttattacag ccgcccagat aaactagaca
      241 aatgttttta caaatactat ggacttaata agttaattaa tttattgata aaagaaaagc
      301 cagatttaat attattaacg tttcctacac cagttatgtc ggtactaact gagcaattta
      361 acattaatat tccagttgct acagtgatga cagactatcg cttacataaa aactggatta
      421 cgccgtattc aacaagatat tatgtggcaa caaaagaaac gaaacaagac ttcatagacg
      481 taggtattga tccttcaaca gttaaagtga caggtattcc tattgataac aaatttgaaa
      541 cgcctattaa tcaaaagcag tggttaatag acaacaactt agatccagat aagcaaacta
      601 ttttaatgtc agctggtgca tttggtgtat ctaaaggttt tgacacgatg attactgata
      661 tattagcgaa aagtgcaaat gcacaagtag ttatgatttg tggtaagagc aaagagctaa
      721 agcgttcttt aacagctaag tttaaatcta acgagaatgt attgattcta ggttatacca
      781 aacacatgaa tgaatggatg gcatcaagtc aacttatgat tacgaaacct ggtggtatca
      841 caataactga aggtttcgcc cgttgtattc caatgatttt cctaaatcct gcacctggtc
      901 aagagcttga aaatgccctt tactttgaag aaaaaggttt tggtaaaatc gctgatactc
      961 cagaagaagc aataaaaatt gtagcaagtt taactaacgg taatgagcaa ttaactaaca
     1021 tgattagtac aatggaacaa gataaaatta agtatgctac acagacaata tgtcgagact
     1081 tacttgattt aatcggtcat tcatcacaac cacaagagat ttatggaaag gttcctttat
     1141 atgcaagatt cttcgttaaa taattacgct aatcataaaa atttcatttt aatgcttatt
     1201 atcttatttt taatggaatt tgcgagaggc atgtatatcc taagctatat taatttcttg
     1261 ccaacagtta catccattgc tgtagcaata acgtctctag cattttcaat acattttatt
     1321 gctgatgcat ctacaaactt tgttatagga tttttactga aaaaattcgg cacaaaaatc
     1381 gttttaacga ctggatttat tttagcattt acaagtttat ttttagttat ttggtttcca
     1441 gcatcacctt ttgtaatcat atttagtgct atgatgctcg gtattgcagt tagtcctatt
     1501 tgggtcatta tgttatccag tgttgaagaa gataaacgtg gcaaacaaat gggctatgtt
     1561 tatttttcat ggttgttagg cctattggta ggtatggttt ttatgaatct acttatcaaa
     1621 gtgcacccaa cacgctttgc cttcatgatg tctttagtcg ttttaattgc atggatatta
     1681 tattattttg tcgatgtaaa gttaacaaat tataatactc gtccagttaa agctcaattg
     1741 agacaaattg ttgatgtgac aaagcgccat ttattgttgt tccctggtat tcttttacaa
     1801 ggtgccgcaa ttgctgccct agttcctata ttaccaacat atgctactaa ggttattaac
     1861 gtaagcacaa ttgaatatac tgttgcaatc attattggtg gtatcggctg tgcagtttcg
     1921 atgctatttt tatcgaaact aatagataac cgcagcagaa actttatgta tggtgttatt
     1981 ctaagcggat ttattttata catgatatta atttttactc tatctatgat tgttaatatt
     2041 cacatcttgt ggattatcgc tttagctatc ggtctaatgt atggcatctt attaccagca
     2101 tggaatacct ttatggcacg atttatcaaa tcagatgaac aagaagaaac ttggggtgtt
     2161 tttaatagta tccaaggatt cggctcaatg atagggccat tatttggcgg attaattaca
     2221 caatttacca acaatttaaa caatacattt tatttctcgg cattaatctt tttagtctta
     2281 gctgtatttt atggaagcta ctttatagta aatagagaaa aagctaagta aggttaaaac
     2341 gtattttaaa ataaagaaaa tggtcatttt tattacattc aataaaaatg accattttct
     2401 tattaattca aacatactac gattaacaat actattatgt tacttaagct aatttaaacg
     2461 tttcaataac tttccattta tcttcgtcat cgtcaaaacg taacaaagtt aattcatcga
     2521 taatttcttt atggtctacc ccagctaatg ctacttgacc aaaaatatct tcgaattctt
     2581 ggctagatag tccttgtgct attgtaaagt gtggcacaaa aacatgttca gcttctccat
     2641 agaaatcttc tccattaaag cgattaaaca attcttctaa gtcgtccgtc ttcgcaactt
     2701 taaagtaaat cacattgttc gttggtttga agctagaagc ttttgtagca tgaacttcta
     2761 ctgctggtat accattaata cgagctctaa cctgttcaat gacagaatct aaatcaccat
     2821 ctttaatttc aaatggcgct ttaattgtca catgtggttt aattcgtgaa tactgtttat
     2881 catatctttt acggtaagaa tccaccgctt cttgaaatga ctttgatgga attaatgcta
     2941 atcctaaaat catctgaatt cctcctttgt ttaacctatt gctattatat caaatttctt
     3001 tgaaaaccgt tacattaaaa ataattcatt atacatcttt tgcaatttcg tcaaatttaa
     3061 attaaattaa acagtaaaat cttttataaa ttatattata gaccagacta tatgaagaca
     3121 attcatacta aatctactaa tgtaaattat tcataaagtt gatcatctgt gtttttactt
     3181 aaaaaataca ataatatatc tgacagcaat ggtttccaat ctttccattg gtgaccacca
     3241 tcaaattcat cgtaataata agttatatta tatttcttaa tttgttcagc taattctcta
     3301 ttcggtgtta agaaatcggc acgcttacca tttgttggta aagtaaaatc tttttcatct
     3361 agaccaatga catgccaaat tgtcaattgt tctttatttg cacattgatt tagcttatct
     3421 aatacttttt catctgaatg tggacttaac attgctacac gactaaaaat cgttggatag
     3481 gtcaacgccg ttaataaggc aatactaccc gctaaactat cccctactaa taaccttgca
     3541 ttacctactt tcagtgtaga aaacgtcgag tcaataaatg gcaatatttc tttaccgact
     3601 gattgaatag ttaaatgaga acgacttcct tgtggatgaa attcctctct acgcttatca
     3661 acgtcttcat aatggaatcc aacaatgatc gcatcatcaa tacgcgcttc tttgattaac
     3721 gattcatatg tacgttgtat tctcccgaaa cgtaaaaaat ctaatccgtc aaagcaaaga
     3781 atgacattat atttaacaag ttgattataa gattctggta aataaatact taacgtga
//
LOCUS       DAKTIO010000207         3788 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.212, whole genome shotgun sequence.
ACCESSION   DAKTIO010000207 DAKTIO010000000
VERSION     DAKTIO010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.212"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            38..1039
                     /gene="argF"
                     /locus_tag="O0293_002504"
     CDS             38..1039
                     /gene="argF"
                     /locus_tag="O0293_002504"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCY0281362.1"
                     /translation="MKNLRNRSFLTLLDFSRQEVEFLLTLSEDLKRAKYIGTEKPMLK
                     NKNIALLFEKDSTRTRCAFEVAAHDQGANVTYLGPTGSQMGKKETTKDTARVLGGMYD
                     GIEYRGFSQRTVETLAEYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKDYADINFTY
                     VGDGRNNVANALMQGAAIMGMNFHLVCPKELNPTDELLNRCKNIAAENGGNILITDDI
                     DQGVKGSDVIYTDVWVSMGEPDEVWKERLELLKPYQVNKEIMDKTGNPNVIFEHCLPS
                     FHNADTKIGQQIFEKYGIREMEVTDEVFESKASVVFQEAENRMHTIKAVMVATLGEF"
     gene            1062..1994
                     /gene="arcC"
                     /locus_tag="O0293_002505"
     CDS             1062..1994
                     /gene="arcC"
                     /locus_tag="O0293_002505"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCY0281363.1"
                     /translation="MAKIVVALGGNALGKSPQEQLELVKNTAKSLVGLITKGHEIVIS
                     HGNGPQVGSINLGLNYAAEHNQGPAFPFAECGAMSQAYIGYQLQESLQNELHSIGMDK
                     QVVTLVTQVEVDENDPAFNNPSKPIGLFYNKEEAEQIQKEKGFIFVEDAGRGYRRVVP
                     SPQPISIIELESIKTLIKNDTLVIAAGGGGIPVIREQHDGFKGIDAVIDKDKTSALLG
                     ANIQCDQLIILTAIDYVYINFNTENQQPLKTTNVDELKRYIDENQFAKGSMLPKIEAA
                     ISFIENNPKGSVLITSLNELDAALEGKVGTVIKK"
     gene            2166..3722
                     /locus_tag="O0293_002506"
     CDS             2166..3722
                     /locus_tag="O0293_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HCY0281364.1"
                     /translation="MENTINESEKKKRFKLKMPGAFMILFILTVVAVIATWVIPAGAY
                     SKLSYEPSSQELKIVNPHNQVKKVPGTQQELDKMGVKIKIEQFKSGAINKPVSIPNTY
                     ERLKQHPAGPEQITSSMVEGTIEAVDIMVFILVLGGLIGVVQASGSFESGLLALTKKT
                     KGHEFMLIVFVSILMIIGGTLCGIEEEAVAFYPILVPIFIALGYDSIVSVGAIFLASS
                     VGSTFSTINPFSVVIASNAAGTTFTDGLYWRIGACIVGAIFVISYLYWYCKKIKNDPK
                     ASYSYEDKDAFEQQWSVLKDDDSAHFTLRKKIILTLFVLPFPIMVWGVMTQGWWFPVM
                     ASAFLIFTIIIMFIAGTGKSGLGEKGTVDAFVNGASSLVGVSLIIGLARGINLVLNEG
                     MISDTILHFSSSLVQHMSGPLFIIVLLFIFFCLGFIVPSSSGLAVLSMPIFAPLADTV
                     GIPRFVIVTTYQFGQYAMLFLAPTGLVMATLQMLNMRYSHWFRFVWPVVAFVLIFGGG
                     VLITQVLIYS"
BASE COUNT         1290 a          541 c          753 g         1204 t
ORIGIN      
        1 aatttaggtg taactaaaat aaaaggagtt gttattaatg aaaaatttac gaaacagaag
       61 ttttttaact ttattagact tttcacgaca agaggtagaa ttcttattaa cactctccga
      121 ggatttaaaa cgtgctaaat atattggcac tgaaaagcct atgttaaaaa ataaaaatat
      181 tgcactgtta tttgaaaaag attctacaag aacgcgatgt gcatttgaag ttgcagcgca
      241 tgatcaaggt gcaaatgtaa cttatttagg cccaactgga tcacaaatgg gtaaaaaaga
      301 aacaactaaa gatactgcac gtgtgcttgg tggaatgtat gatggcattg aataccgtgg
      361 tttttcacaa agaacagtag aaactttagc tgagtattca ggcgtaccag tgtggaatgg
      421 tttaactgat gaagatcatc ctactcaagt tcttgctgat ttcttaacag caaaagaagt
      481 cttaaaaaaa gattatgcag atattaactt tacatatgtt ggagatggtc gtaataacgt
      541 tgcaaatgca ttaatgcaag gtgctgccat tatgggtatg aacttccatt tagtttgtcc
      601 aaaagaatta aatccaacag atgaattatt aaatcgctgt aaaaatattg ccgctgaaaa
      661 tggtggcaac atattaatca cagatgatat tgaccaaggt gtaaaaggtt cggatgtaat
      721 ttacactgat gtttgggtat caatgggtga acctgatgaa gtatggaaag aacgacttga
      781 attattgaaa ccatatcaag taaataaaga aataatggat aaaactggta atccaaatgt
      841 tatttttgag cattgcttac catctttcca taatgctgat acgaaaatag gtcaacaaat
      901 ttttgaaaaa tatggtattc gagaaatgga agttacagat gaagtattcg aaagtaaagc
      961 ttcagttgta ttccaagaag ctgagaacag aatgcataca atcaaagcag tcatggttgc
     1021 tacattgggt gaattttaaa tgatataagg aagtgaatat gatggcgaaa atagtagtag
     1081 cattaggtgg taatgcttta ggaaaatcac ctcaagaaca actcgagctt gttaaaaata
     1141 ctgcgaaatc attagtagga ttaataacaa aaggacatga gattgttatt agtcatggta
     1201 atggaccaca ggttggaagc attaatttgg gacttaacta tgctgcagaa cataaccaag
     1261 gtccggcatt tccatttgct gaatgtggcg caatgagtca agcttacatc ggctatcaat
     1321 tacaagaaag cttacaaaat gaattgcatt ctattggaat ggataaacaa gtggtaacac
     1381 tagtgacaca agttgaagtt gatgaaaatg atccggcatt taacaatcct tcaaaaccaa
     1441 ttgggttatt ttacaacaaa gaagaagctg aacaaattca aaaagaaaaa ggatttatat
     1501 ttgttgaaga tgctggaaga ggatatagac gcgttgttcc ttcaccacaa cccatctcta
     1561 ttattgaatt agagagtatt aaaacactta ttaaaaatga tacactcgtt attgctgctg
     1621 gtggtggagg tataccagta attagagagc aacatgatgg ttttaaaggt attgatgcag
     1681 ttatagacaa agataaaaca agtgcgttgt tgggtgctaa tattcaatgc gatcaattga
     1741 ttattttaac agcaattgat tatgtatata ttaattttaa cactgaaaac caacagcctt
     1801 tgaaaacaac aaatgttgat gaattaaaac gatatataga cgaaaatcaa tttgcaaaag
     1861 gaagtatgtt accaaaaatt gaagcagcca tatcatttat tgaaaacaat ccaaaaggaa
     1921 gtgtgcttat aacatcatta aatgaattag atgctgcctt agagggtaaa gtaggtactg
     1981 tgattaaaaa gtaattgaat tgaaacgctt ttcaattact atatgtcaaa tgcatgattt
     2041 ttattattta tgtgcacccc ctgaaaataa tgcctctatt ttgatgcggg gtgcactttc
     2101 ttaatttata tttataaaat ctttaaggta gaaatgctag gttaaatgtc gaaggagatg
     2161 aaaccgtgga aaatacaatt aatgaaagtg aaaagaaaaa acgatttaaa ttaaaaatgc
     2221 caggtgcatt tatgatttta ttcattttaa cggttgttgc agttatagca acatgggtta
     2281 ttcctgctgg tgcatattct aaactttctt acgaaccttc atcccaagaa ctaaagatag
     2341 ttaaccctca taaccaagtg aaaaaggttc cgggtacgca acaggaacta gacaaaatgg
     2401 gggttaaaat taagattgaa caatttaaat caggtgcaat taataagccg gtatcaattc
     2461 cgaatactta tgaaagatta aagcaacatc cagctggacc agaacaaata acaagtagca
     2521 tggttgaagg tacgatagaa gcggtcgata tcatggtatt cattcttgta ctagggggac
     2581 ttattggcgt agttcaagcc agtggttctt ttgaatcggg attgttagct ttaacgaaga
     2641 aaacaaaagg gcatgaattt atgctaattg tgtttgtatc aatactaatg attatcggcg
     2701 ggacattatg tggtattgaa gaagaagctg tagcatttta tccgatttta gtccctatat
     2761 ttatagcgtt aggatacgat tctatcgttt cagttggtgc catattcctt gccagctctg
     2821 tcggtagtac attttcaact attaacccgt tctcggttgt aattgcctct aatgccgctg
     2881 gtacaacttt tacggatggc ttgtattgga gaataggtgc ttgtattgtc ggtgcgattt
     2941 ttgttattag ttatttatat tggtactgta aaaaaattaa aaacgatcct aaagcgtcat
     3001 attcttatga agacaaagat gcttttgaac agcaatggtc tgtattaaaa gatgacgata
     3061 gtgcccattt tactttgcgt aagaagataa tccttacatt atttgtacta ccatttccaa
     3121 ttatggtatg gggagttatg acgcaaggtt ggtggttccc agttatggct tcagcatttt
     3181 taatatttac aattataata atgtttattg ctgggacagg taaatctgga ttgggagaaa
     3241 aaggaactgt agatgcattt gtcaatggtg catcaagttt agtaggtgta tctttaatta
     3301 ttggtttagc tcgaggtatt aatttagtgt tgaatgaagg tatgatttca gatacaatct
     3361 tacacttttc atcatcttta gttcaacata tgagtggacc attatttatc atcgtattac
     3421 tatttatttt cttctgttta ggttttatcg tgccatcatc ttctggctta gcggtattat
     3481 caatgcctat ctttgcacca ctagctgata cagtaggtat accaagattc gtcatcgtta
     3541 cgacatatca atttggtcaa tatgcgatgt tattcttagc gccgactgga cttgttatgg
     3601 ccacactaca aatgttaaac atgcgatatt cacattggtt ccgatttgta tggccggtag
     3661 ttgcttttgt attgattttc ggtggcggag tactaattac gcaagtacta atttattcat
     3721 aatttgaaat gctatattat aaaaaatact aatggggttt tatgcatctc gtaggtttgt
     3781 agaaatac
//
LOCUS       DAKTIO010000208         3779 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.213, whole genome shotgun sequence.
ACCESSION   DAKTIO010000208 DAKTIO010000000
VERSION     DAKTIO010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.213"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            492..3014
                     /gene="mprF"
                     /locus_tag="O0293_002507"
     CDS             492..3014
                     /gene="mprF"
                     /locus_tag="O0293_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCY0281365.1"
                     /translation="MNQEVKNKIFSILKITFATALFIFVAITLYRELSGINFKDTLVE
                     FSKINRMSLVLLFIGGGASLVILSMYDVILSRALKMDISLGKVLRVSYIINALNAIVG
                     FGGFIGAGVRAMVYKNYTHDKKKLVHFISLILISMLTGLSLLSLLIVFHVFDASLILD
                     KITWVRWVLYVVSFFLPLFIIYSMVRPPDKNNRFVGLYCTLVSCVEWLAAAVVLYFCG
                     VIVDAHVSFMSFIAIFIIAALSGLVSFIPGGFGAFDLVVLLGFKTLGVPEEKVLLMLL
                     LYRFAYYFVPVIIALILSSFEFGTSAKKYIEGSKYFIPAKDVTSFLMSYQKDIIAKIP
                     SLSLAILVFFTSMIFFVNNLTIVYDALYDGNHLTYYILLAIHTSACLLLLLNVVGIYK
                     QSRRAIIFAMISILLITVATFFTYASYILITWLAIIFVLLIVAFRRARRLKRPVRMRN
                     IVAMLLFSLFILYVNHIFIAGTLYALDIYTIEMHTSVLRYYFWLTILIIAIIIGMIAW
                     LFDYQFSKVRISSKIEDCEEIINQYGGNYLSHLIYSGDKQFFTNENKTAFLMYRYKAS
                     SLVVLGDPLGDENAFDELLEAFYNYAEYLGYDVIFYQVTDQHMPLYHNFGNQFFKLGE
                     EAIIDLTQFSTSGKKRRGFRATLNKFDELNISFEIIEPPFSTEFINELQHVSDLWLDN
                     RQEMHFSVGEFNEEYLSKAPIGVMRNEENEVIAFCSLMPTYFNDAISVDLIRWLPELD
                     LPLMDGLYLHMLLWSKEQGYTKFNMGMATLSNVGQLHYSYLRERLAGRVFEHFNGLYR
                     FQGLRRYKSKYNPNWEPRFLVYRKDNSLWESLSKVMRVIRHK"
     gene            complement(3217..3726)
                     /gene="msrA"
                     /locus_tag="O0293_002508"
     CDS             complement(3217..3726)
                     /gene="msrA"
                     /locus_tag="O0293_002508"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCY0281366.1"
                     /translation="MNINTAYFAGGCFWCMTKPFDTFDGIEKVTSGYMGGHIENPTYE
                     QVKSGTSGHLETVEIQYDVALFSYNKLLEIFFSVIDPLDTGGQYQDRGPQYQTAIFYT
                     NDHQKELAETYIEQLKNTINADKAIATKILPASQFYKAEDYHQDFYKKNPERYAEEQK
                     IRQEYKNKQ"
BASE COUNT         1167 a          510 c          643 g         1459 t
ORIGIN      
        1 tagttgaata agtaataaaa aataccaatg actatatgtt tgttggtatt ttttatggtg
       61 cttataatgt tgggcagtta catttatgat tatcagcaca tgtttagctt ataaattttt
      121 acgaaagttt agccgtttat aaagcacatg cataatgaaa cgagtatttg ccacttgatt
      181 agtacttcat tattatgtcg aaaataaaaa taagtggtat ttttaatata ttaagaagca
      241 ctcataatcg gctgttaatt aataatattt ttcataagta ttgattcatc attttcttta
      301 tgttaaatat gaatcagtat agataaccat attgttctgt ttgagataga aatattttct
      361 atttacttct taatacagat atcaatatga caaaagtgtt atataaatca aaggtaaatg
      421 atatgctatt tttatggttt atatgatata ttttgattta taacagaaat aattagaatt
      481 gatgtgaaaa aatgaatcag gaagttaaaa acaaaatatt ttcaatctta aaaattacgt
      541 ttgctacagc tttatttatt tttgtagcaa tcacattgta tcgggagtta tctggtatta
      601 actttaaaga tacgttggtt gaatttagta agattaaccg tatgtcctta gtgttactat
      661 ttattggtgg tggggcatcg cttgttattc tatcaatgta tgatgtgatt ttatctagag
      721 ctttaaaaat ggatatatcc ttaggcaaag ttttaagagt aagttatatc atcaatgcat
      781 tgaatgcgat tgtaggtttc ggtggcttta ttggtgcagg cgttagagca atggtttata
      841 aaaactatac gcatgataaa aagaaattag ttcactttat atccttaata cttatttcaa
      901 tgttgacagg tttaagctta ttatcattgc taattgtatt ccatgttttc gatgcatctt
      961 taatcttaga taagattaca tgggtaagat gggtattata tgtagtgtca tttttcttac
     1021 cattattcat tatttattca atggttagac cacccgataa aaacaatcgt tttgtaggat
     1081 tgtactgcac tttagtgtcg tgtgttgaat ggttagcagc tgcagttgta ttatatttct
     1141 gtggtgtaat tgttgacgct catgtatcat tcatgtcctt tattgcaata tttatcattg
     1201 ctgcattatc aggtttagtc agctttattc ctggtggttt cggcgctttc gatttagttg
     1261 tattactagg atttaaaact ttaggtgtcc ctgaggaaaa agtattatta atgctacttc
     1321 tatatcgttt tgcgtactat tttgtaccgg taattattgc attaatttta tcatcatttg
     1381 aatttggtac atcagctaag aagtacattg agggatctaa atactttatt cctgctaaag
     1441 atgttacgtc atttttaatg tcttatcaaa aggatattat tgctaaaatt ccatcattat
     1501 cattagcaat tttagtattc tttacaagta tgatcttttt tgtaaataac ttaacgattg
     1561 tttacgatgc tttatatgat ggaaatcact taacgtatta tattctattg gcaattcata
     1621 ctagtgcttg tttattactt ttactgaatg tagttggtat ttataagcaa agtagacgtg
     1681 ccattatctt tgctatgatt tcaattttat taatcacagt ggcgacattc ttcacttacg
     1741 cttcatatat tttaataaca tggttagcta ttatttttgt tctgcttatt gtagctttcc
     1801 gtagagcacg taggttgaaa cgcccagtaa gaatgagaaa tatagttgca atgcttttat
     1861 tcagtttatt tattttatat gttaaccata tatttattgc tggaacgtta tatgcattag
     1921 atatttatac gattgaaatg catacatctg tattgcgcta ttacttctgg cttacgattt
     1981 taatcatcgc tatcatcata ggtatgattg catggttgtt tgattatcaa tttagcaaag
     2041 tacgtatttc ttctaaaatt gaagattgcg aggagattat taatcagtac ggcggtaatt
     2101 atttgagtca cttgatatat agtggtgaca agcagttttt cactaatgaa aataaaacag
     2161 catttttaat gtatcgttat aaagcaagtt cattagtggt tcttggagat ccgttaggtg
     2221 atgaaaatgc ctttgatgaa ttgttagaag cattctataa ttacgctgag tatttaggct
     2281 atgatgttat attctatcaa gttacagatc aacacatgcc tttatatcat aatttcggta
     2341 accaattttt caaattaggt gaagaagcaa ttattgattt aacgcaattt tcaacttcag
     2401 gtaaaaaacg ccgtggattt agagcgactt taaataaatt cgatgaactt aatatttcgt
     2461 tcgaaattat tgaaccaccg ttttcaactg aatttataaa tgaacttcaa catgtaagtg
     2521 atttatggct agataatcgt caggaaatgc atttctctgt tggtgaattt aatgaagaat
     2581 acttatctaa agcgccaatt ggtgtaatgc gaaatgaaga aaatgaagta attgcatttt
     2641 gtagtttaat gccaacatac tttaatgatg ccatttcagt cgatttaatt agatggttgc
     2701 cagagttaga tttaccatta atggatggtc tatacttgca tatgttactt tggagtaaag
     2761 aacaaggtta tacaaaattt aatatgggta tggcaacgtt atcgaacgtt ggtcaattgc
     2821 attattcata tttaagagaa cgacttgcag gccgtgtctt tgaacatttc aacggtctat
     2881 atcgtttcca aggattacgt cgttataaat ctaaatataa tccgaattgg gaaccacgct
     2941 ttttagttta tcgtaaagat aattcgcttt gggaatcact ttctaaagta atgcgtgtaa
     3001 tacgtcacaa ataattaaaa tccaagtgct aagaggtata cagttatgcc tgatgcgctt
     3061 ggattttttg gtttctataa agaatgattt ataaaaaacg gtacagataa gaatatttaa
     3121 gtttcctatc tgtaccgtat tttaatattg aaatgagcta aagttaagtc gcctaacttt
     3181 tattatcagt ccaatcagtt tcatcaagta taaatgttat tgcttatttt tgtattcttg
     3241 gcgtattttt tgttcttctg catagcgctc tggatttttc ttataaaaat cttggtgata
     3301 gtcttcggct ttgtaaaatt gtgacgctgg taatattttt gttgcaattg ccttatcagc
     3361 attaatcgta tttttaagct gctcgatata agtctcagcg agttcttttt gatgatcatt
     3421 agtgtagaaa atagctgttt gatattgagg accacggtct tgatattgac cacctgtatc
     3481 taatgggtca atgactgaga aaaatatttc taataactta ttgtatgaga ataatgcaac
     3541 atcatattga atttcaacag tttctaaatg accactcgta cctgatttta cttgttcgta
     3601 agtaggattt tcaatatgtc cgcccatata tccagaagtt actttttcta tgccgtcaaa
     3661 ggtgtcaaat ggtttcgtca tacaccaaaa gcaacctccg gcaaaataag ctgtattaat
     3721 attcattttt gacatccttt cattagacct tagtacgatt tattaagaaa tcacttgct
//
LOCUS       DAKTIO010000209         3750 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.214, whole genome shotgun sequence.
ACCESSION   DAKTIO010000209 DAKTIO010000000
VERSION     DAKTIO010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.214"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(134..319)
                     /locus_tag="O0293_002509"
     CDS             complement(134..319)
                     /locus_tag="O0293_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281367.1"
                     /translation="MLSNKRVEKEAGCEHKKFLKKLLTLNSKVDNMIKSSKTTQLFLK
                     KNFKSVKLTIEITIKTI"
     gene            complement(408..1472)
                     /locus_tag="O0293_002510"
     CDS             complement(408..1472)
                     /locus_tag="O0293_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HCY0281368.1"
                     /translation="MLTVDQVKELVGEIKDPIIDVPLKETEGIVEVSIKEEKEHVSVK
                     LAMAQLGGAPQLDLQMAVVNALKENGAKTVGIRFETLPEEKVNQFKPKEENKPKTIEG
                     LLSQNNPVEFIAIASGKGGVGKSTVAVNLAVALAREGKKVGLVDADIYGFSVPDMMGI
                     DEKPGIKGKEVIPVERHGVKVISMAFFVEENAPVIWRGPMLGKMLTNFFTEVKWGDIE
                     YLILDLPPGTGDVALDVHTMLPSSKEIIVTTPHPTAAFVAARAGAMAKHTDHSILGVI
                     ENMSYFESKETGNKEYVFGKGGGTKLADELNTQLLGELPLEQPSWNPKDFAPSIYQSD
                     DRLGKIYSSIAQKVIAATNK"
     gene            complement(1620..3062)
                     /gene="lmrS"
                     /locus_tag="O0293_002511"
     CDS             complement(1620..3062)
                     /gene="lmrS"
                     /locus_tag="O0293_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter LmrS"
                     /protein_id="HCY0281369.1"
                     /translation="MAKVELTTRRRNFIVAVMLISAFVAILNQTLLNTALPSIMRELN
                     INESTSQWLVTGFMLVNGVMIPLTAYLMDRIKTRPLYLAAMGTFLLGSIVAALAPNFG
                     VLMLARVIQAMGAGVLMPLMQFTLFTLFSKEHRGFAMGLAGLVIQFAPAIGPTATGLI
                     IDQASWRVPFIIILGIAILAFVFGLVSISSYNEVKYTKLDKRSVMYSTIGFGLMLYAF
                     SSAGDLGFTSPIVIGALILSMVIIYLFIRRQFNITNALLNLRVFKNRTFALCTISSMI
                     IMMSMVGPALLIPLYVQNSLSLSALLSGLVIMPGAIINGIMSVFTGKFYDKYGPRPLI
                     YTGFTILTITTIMLCFLHTDTSYTYLIVVYAIRMFSVSLLMMPINTTGINSLRNEEIS
                     HGTAIMNFGRVMAGSLGTALMVTLMSFGAKIFLSTSPSHLTATEIKQQSIAIGVDISF
                     AFVAVLVMAAYVIALFIREPKEIESNRRKF"
     gene            complement(3384..>3750)
                     /gene="sepA"
                     /locus_tag="O0293_002512"
     CDS             complement(3384..>3750)
                     /gene="sepA"
                     /locus_tag="O0293_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux transporter SepA"
                     /protein_id="HCY0281370.1"
                     /translation="GLYGLSRILIYFRLGDFTYNRSMYDNLLYYGSYIIFGYFIIFAV
                     EHLMDYFRKMLPENAYFRGATFHLISYTVATTLFYFIIHLNYVYINIDFWVIMVIIGF
                     LYVCKLQFYPESKNLNNRK"
BASE COUNT         1280 a          725 c          485 g         1260 t
ORIGIN      
        1 atagttacgt ttttggaatt aacgttgaca tattgtcatt cagttttcaa tgttcattaa
       61 tgttcaatct cttttattct acttcattta tttcttgaag tcaataactt tttgaacgat
      121 tactttattt attctatatt gtttttatag ttatttcaat ggtaagtttt acacttttga
      181 aattcttctt taaaaacaac tgcgtcgttt ttgacgactt tatcatatta tcaactttgg
      241 aatttaaagt caataacttt tttaaaaact ttttgtgttc acaacccgct tctttttcaa
      301 cgcgtttatt gcttaacaca agaacttatt ttaccagcat tccaaaacaa atcaacataa
      361 aaacgtacaa aataaaagta attttgtacg tttagcatat attataccta tttatttgta
      421 gcagctataa ctttttgtgc aatcgagcta taaattttac ctagacgatc atctgattga
      481 tatattgacg gtgcaaaatc ttttggattc caagatggtt gctctaaagg taattcccca
      541 agtaattgag tattaagttc atcagctaac ttagtaccgc cacctttgcc aaagacatat
      601 tctttattac ccgtctcttt actttcaaaa taactcatgt tttcaattac gccaagaata
      661 gaatgatccg tatgttttgc catcgcacct gcgcgagctg caacaaatgc tgctgtagga
      721 tgaggtgtcg ttacaataat ttccttactt gaaggtaaca tcgtatgaac atctaaagct
      781 acatctcctg ttccaggtgg aagatcgagt attaaatatt caatgtctcc ccatttaact
      841 tctgtaaaga aattcgtcaa cattttacct aacattggcc ctctccatat aactggcgca
      901 ttttcttcca caaaaaaggc cattgatata actttaacgc catgacgttc aactggaatt
      961 acttccttcc ctttaattcc aggcttttca tcaataccca tcatatctgg tacactaaat
     1021 ccatatatat cggcatctac taatccgact tttttccctt cacgagctaa ggcaacggct
     1081 aaatttactg caacagtaga tttaccgaca ccacctttac cggaggctat tgcaataaat
     1141 tcgactggat tattttgaga taataggcct tctatcgttt taggtttatt ttcttctttt
     1201 ggtttaaatt gatttacttt ttcttccggc aatgtttcaa atcgtatacc gaccgttttc
     1261 gcaccgtttt cttttaatgc attaacaaca gccatctgta aatctaattg cggtgcacca
     1321 cctaattgtg ccattgctaa tttaacactc acatgttctt tttcttcctt aatagaaact
     1381 tcaacaatac cttctgtttc ttttaaaggc acatctataa taggatcttt aatttctcct
     1441 accaattctt tcacttgatc taccgttaac aaagcaaatc ccccttaatc attttaagac
     1501 atctacatca tagcaaataa caatttttgc ttagtctatc actaaaagta taaaactttt
     1561 tattaaataa aaaaagcagt tagcctatta ttttaaatag accaactact ataattattt
     1621 taaaatttcc ttctattact ttctatttct ttaggttctc ttataaaaag tgctatcaca
     1681 taagctgcca taacaagcac agctacaaaa gcaaatgaga tatccacccc tatagcaatg
     1741 gattgctgtt taatttcagt tgcagttaaa tgcgatggcg atgtagataa aaatattttt
     1801 gcaccaaaac tcattaatgt aaccattaaa gctgtgccta gtgaaccagc cattacacga
     1861 ccaaagttca taatagccgt gccatgtgag atttcttcat ttctcaaaga attaattcct
     1921 gtagtattta tcggcatcat gagtaaagaa actgaaaaca ttctaattgc atatacgact
     1981 attaaatacg tataagatgt gtctgtatgc aagaaacaca acataattgt agtaattgtt
     2041 aaaattgtaa aaccagtata aatcaatggt ctaggaccat acttatcata aaatttacct
     2101 gtaaaaactg acataatacc atttattatt gcaccaggca tgataacaag tcctgataac
     2161 aaggcagata aagataaact gttttgaaca tatagcggta taagcagcgc aggtccaacc
     2221 attgacatca ttataatcat tgaactaatc gtacataatg caaatgttct atttttaaaa
     2281 acccttaaat ttaaaagtgc attagtaata ttaaattgtc tacgtataaa taaatagata
     2341 ataaccatac ttaatatcaa cgcacctatt actattggac ttgtaaatcc taaatctcct
     2401 gcactactaa atgcgtatag cattaacccg aacccaatag ttgaatacat tactgaacgc
     2461 ttatctaatt tcgtatattt cacttcattg taactcgaga ttgaaaccaa accgaaaaca
     2521 aaggcaagta tagcaattcc taaaattata ataaatggaa ctcgccaact cgcttgatca
     2581 ataattaatc ctgtagctgt aggtcctatt gctggtgcaa attgaattac taaacctgct
     2641 agtcccattg caaaacctcg atgttcttta ctgaacaatg taaataacgt aaattgcatt
     2701 aagggcataa gtacgcctgc acccatcgct tgaattacac gagctaacat taaaactcca
     2761 aaattcggag ctaaggctgc aacaatagaa cctaataaaa atgtccccat cgccgctaag
     2821 tataaaggtc tagttttaat tctatccatt agatatgccg tcagaggtat catgacgcca
     2881 ttaacaagca taaacccagt aactagccat tgcgatgtac tttcattgat atttaattct
     2941 ctcattatac taggtaacgc tgtatttaat aatgtttgat ttaaaatagc tacaaacgca
     3001 ctaatcaaca taacagcaac tatgaagttt cgtctccggg ttgttaattc aactttagcc
     3061 atcataattc ctccttttta aaacagatat acacttgtcc ctttaatacg tatagccatg
     3121 aacataatgg gagtagtact caaataataa agaagcacta cataataaat cattattggt
     3181 tcttacacat taggcacagc taatgtgtac ttaaaaacta acaatataac attgtagggc
     3241 ctaataattt attttatgtc tcagactcat acaatggtta tttaacttat ttaccacaaa
     3301 ttgagcacta agactcacta ttatcgattc ttctattgaa caaaaagaag ttggaacaca
     3361 tatgtgccct caacttcaga tatctatttt ctattattta aatttttact ttcagggtaa
     3421 aattgaagct tacaaacata taagaaccct ataataacca tgatgaccca aaaatcgata
     3481 ttgatataca catagttcag atgaataata aaataaaata acgttgttgc aactgtgtaa
     3541 gaaatcaaat gaaaagtcgc gcctctaaaa tatgcatttt caggcaacat ttttctaaaa
     3601 taatccatta aatgctcgac tgcaaatatg atgaagtacc caaagattat ataactgcca
     3661 taatataata ggttatcgta catacttcta ttatatgtaa agtcacccaa cctaaaataa
     3721 ataagtattc tacttaatcc atataaacca
//
LOCUS       DAKTIO010000210         3746 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.215, whole genome shotgun sequence.
ACCESSION   DAKTIO010000210 DAKTIO010000000
VERSION     DAKTIO010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.215"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            184..552
                     /locus_tag="O0293_002513"
     CDS             184..552
                     /locus_tag="O0293_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCY0281371.1"
                     /translation="MISNDAIRRNMAVFSMSVVSKLTDLTPRQIRYYETHELIKPERT
                     EGQKRLFSLNDLERLLEIKSLLEKGFNIKGIKQIIYDSQEHLTTDEQEIRKKMIVDAT
                     QKPIGETLPINRGDLSRFIK"
     gene            571..1911
                     /gene="glnA"
                     /locus_tag="O0293_002514"
     CDS             571..1911
                     /gene="glnA"
                     /locus_tag="O0293_002514"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HCY0281372.1"
                     /translation="MPKRTFTKDDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQL
                     EKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTD
                     GTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFD
                     LAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKL
                     VVKTIARKHNLHATFMPKPLFGVNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQF
                     TAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRI
                     EVRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPS
                     TLYTALKAMRENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY"
     gene            2404..2601
                     /locus_tag="O0293_002515"
     CDS             2404..2601
                     /locus_tag="O0293_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281373.1"
                     /translation="MNCYDEIFNTIKELIENKEISSYQINKDTGISYGNINAMRRRER
                     RIENLSLKNAKILYEYAKKVL"
     gene            3302..3514
                     /locus_tag="O0293_002516"
     CDS             3302..3514
                     /locus_tag="O0293_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281374.1"
                     /translation="MNQFKEIYNTIEKLLNDKSISNYRINQDTDVSYGGISELRSGKR
                     KVNNLTLETAEKLYNYQKQLEIMIED"
BASE COUNT         1453 a          478 c          648 g         1167 t
ORIGIN      
        1 atcagattat ttttaaaaat tgaaaaaata ttttagctgt agattgtttt atgtcgtttt
       61 agaagtcgaa atcgcagtta tatcaatgtt ttaagcttta tgttagaaaa cctgacatat
      121 ttttgaaatc ctaaaaaaat tatgataagt tattaacaag ttcaaaagta gaggagagga
      181 acaatgatat cgaatgatgc aatcagacga aatatggctg tcttctctat gagtgtagta
      241 agtaagttaa cggatttaac gccaaggcaa atacgttact atgaaacaca tgaactcatc
      301 aaacctgaaa gaacagaagg tcaaaaacgt ctgttctcac tcaatgattt ggaaagatta
      361 ctagaaatta aatcattatt agaaaaagga tttaatatca aagggattaa acaaatcatt
      421 tatgactcac aagagcattt aacaacagat gaacaagaga taagaaaaaa gatgattgta
      481 gatgccacgc aaaagcctat tggagaaact ttgccaataa atcgtggtga tttatcccga
      541 tttattaaat aaaatttgga ggattttaaa atgccaaaac gtactttcac taaagacgac
      601 attcgtaaat ttgcagaaga ggaaaatgta agatatttaa gattacaatt cactgatatt
      661 ttaggaacaa ttaaaaatgt tgaagtgcct gtaagccaat tagaaaaagt acttgataac
      721 gaaatgatgt ttgacggttc ttctatcgaa ggtttcgtac gtatcgaaga atcagatatg
      781 tacttacatc cagatttaga tacttgggta atcttcccat ggactgctgg acaaggtaaa
      841 gttgcacgtt taatttgtga tgtatataaa acagatggaa caccatttga aggggatcct
      901 cgtgcaaact taaaacgtgt attaaaagaa atggaagatt taggcttcac agactttaac
      961 ctagggcctg aaccagaatt cttcttgttt aagttggatg aaaaagggga accaacttta
     1021 gaacttaatg atgatggtgg atatttcgat ttagcaccta cagatttagg tgaaaactgt
     1081 cgtcgtgata ttgttttaga attagaggat atgggcttcg atattgaagc tagtcaccat
     1141 gaagttgccc ctggtcaaca tgaaattgac tttaaatatg cagatgctgt tacagcatgt
     1201 gataatatcc aaacatttaa attggttgtt aaaacaatcg cacgtaaaca taatttacac
     1261 gcaacattta tgcctaaacc attattcggt gtgaatggta gcggtatgca ctttaacgtt
     1321 tcattattca aaggtaaaga aaatgcattc tttgatccaa atactgaaat gggcttaacg
     1381 gaaactgcat atcaatttac agcaggtgtg cttaaaaatg cacgcggatt tactgctgta
     1441 tgtaacccgt tagtaaactc atataaacgt ttagtacctg gttatgaagc accatgttat
     1501 attgcatgga gtggtaaaaa ccgttcacca ttaatccgtg taccatcttc aagaggatta
     1561 tctactcgta tcgaagtacg ttcagtagat ccagctgcaa acccatacat ggcgttagct
     1621 gcaatcttag aagctggact agatggtatt aaaaataaat taaaagttcc agaaccagtt
     1681 aaccaaaata tttacgaaat gaaccgtgaa gaacgtgaag cagtaggcat tcaagactta
     1741 ccttcaacac tttatactgc attaaaagca atgcgtgaaa atgaagttat taaaaaagct
     1801 ttaggaaatc atatctataa tcaatttatt aattcaaaat caattgaatg ggattactac
     1861 agaactcaag tctctgaatg ggaaagagat cagtacatga agcaatatta atacgctaaa
     1921 caatgatggg aaatcagtaa tatcaagggc tagagcattt tgctttagct cttttttgat
     1981 ttggaaagat gggatttcac ttgaccactt ttgttaaaaa atggatttgg tcaagttttg
     2041 gtcaagtaaa tgttttatgt atttattttt ttaaagctgg ggaagaccat tttatatttt
     2101 agttcacatg aattttaata tatttggagg tgatgagatg aggcgataaa agaacgtcgc
     2161 gaaaacgttg agtattactt gaaaatagcc atattcatat tacttctcat cgaattcatt
     2221 tgcagaacgt tctaaggtaa gaccccgaaa agagggacct tactacgcat tattaagggg
     2281 gtaatactat atgaaaagag aaacaaaaca aaaaatatct ttttgtttat cgatcggtat
     2341 tttcatttta gttatactac tattgatatt ctaaaaaatt aaaaatttcg cctcatctaa
     2401 tttatgaatt gctatgatga aatattcaat acaatcaaag aattgataga aaacaaagag
     2461 atatcgagtt atcaaattaa taaagatact gggataagtt acggtaatat taatgctatg
     2521 cgccgtagag aaagaagaat agaaaattta agcttaaaga atgcaaagat cttatatgaa
     2581 tatgcgaaaa aggtattgta atataagcat tgagttcaat aggcatacaa taactgccca
     2641 aactagtgac tgagtgggtg cttttatagt attaaaagtt ttctaaaagt gaaagaaatc
     2701 atgtttagtg atttacttat gtttttcaaa atagtataat aattttatat aatatataga
     2761 gcttcagtat accttttgag tatatatgga gttctttctt tttatacaag atgtatatgt
     2821 ataaatggaa atatttaata cattacataa aaacctttag gtgcacttag agggatgtta
     2881 atcataaaaa aagagatttt gaaaaatata ggctattata ctttagaatt acaaattata
     2941 aatcttgcca gtggatatgg gagttcaaca tgtcacttac cccaatttgt ggaatgttaa
     3001 ataactataa agaggaaagg agaaaaaatg ataagaatgg ttaaagaaaa agaacgaata
     3061 gcaaaattaa atttcgaata gctgtgacga gcttaataat tcaagtactt aattttatca
     3121 aatcgttctt ttaaaaaggt aaagggcaaa agacctttac cacattaaat agggaggtgg
     3181 tatttatgac aagagaatta aggaaaaaat taactctcta tcttaacatt gcaactttaa
     3241 tattatttat tattaattta actagaaaaa aataaattat tctttcattt taacaaaaat
     3301 tatgaaccag tttaaagaaa tttataatac aatagaaaaa ttactaaatg ataaatcaat
     3361 atctaattat agaattaatc aagacactga tgtttcttat ggtggtataa gtgaattaag
     3421 aagcgggaaa agaaaagtga ataatttaac tttagaaaca gcggaaaaac tctataatta
     3481 ccaaaaacaa ttagaaataa tgattgaaga ttaaaataag gccatatttt tgaaatatca
     3541 tggagtgagc cttttaagtc atctaatttt aaagtatatt aaatatggat aacttaatgt
     3601 gaatataaat aaaaaattat caaataatat aatatagaat agatggagac aagaaatttt
     3661 atcaatatat aaaaaaaacg ccaatactat taggtattag tttatgaatt aactagtttg
     3721 gcaactagaa aaaaacaatg tattgc
//
LOCUS       DAKTIO010000211         3725 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.216, whole genome shotgun sequence.
ACCESSION   DAKTIO010000211 DAKTIO010000000
VERSION     DAKTIO010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.216"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            70..285
                     /locus_tag="O0293_002517"
     CDS             70..285
                     /locus_tag="O0293_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281375.1"
                     /translation="MSINIDPEKFAELVVMSNPSKFEDAEDIAKESLKLYINAYRLAE
                     RYSTIATNCYDTAEVIKELKKTDLQLK"
     gene            complement(582..2141)
                     /gene="rny"
                     /locus_tag="O0293_002518"
     CDS             complement(582..2141)
                     /gene="rny"
                     /locus_tag="O0293_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HCY0281376.1"
                     /translation="MNLLSLLLILLGIILGVVGGYVVARNLLLQKQSQARQTAEDIVN
                     QAHKEADNIKKEKLLEAKEENQILREQTEAELRERRSELQRQETRLLQKEENLERKSD
                     LLDKKDEILEQKESKIEEKQQQVDAKESSVQTLIMKHEQELERISGLTQEEAINEQLQ
                     RVEEELSQDIAVLVKEKEKEAKEKVDKTAKELLATAVQRLAADHTSESTVSVVNLPND
                     EMKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARTALVNLVSDG
                     RIHPGRIEDMVEKARKEVDDIIREAGEQATFEVNAHNMHPDLVKIVGRLNYRTSYGQN
                     VLKHSIEVAHLASMLAAELGEDETLAKRAGLLHDVGKAIDHEVEGSHVEIGVELAKKY
                     GENETVINAIHSHHGDVEPTSIISILVAAADALSAARPGARKETLENYIRRLERLETL
                     SESYDGVEKAFAIQAGREIRVIVSPEEIDDLKSYRLARDIKNQIEDELQYPGHIKVTV
                     VRETRAVEYAK"
     gene            complement(2495..3538)
                     /gene="recA"
                     /locus_tag="O0293_002519"
     CDS             complement(2495..3538)
                     /gene="recA"
                     /locus_tag="O0293_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCY0281377.1"
                     /translation="MDNDRQKALDTVIKNMEKSFGKGAVMKLGDNIGRRVSTTSTGSV
                     TLDNALGVGGYPKGRIIEIYGPESSGKTTVALHAIAEVQSNGGVAAFIDAEHALDPEY
                     AQALGVDIDNLYLSQPDHGEQGLEIAEAFVRSGAVDIVVVDSVAALTPKAEIEGEMGD
                     THVGLQARLMSQALRKLSGAISKSNTTAIFINQIREKVGVMFGNPETTPGGRALKFYS
                     SVRLEVRRAEQLKQGQEIVGNRTKIKVVKNKVAPPFRVAEVDIMYGQGISKEGELIDL
                     GVENDIVDKSGAWYSYNGERMGQGKENVKMYLKENPQIKEEIDRKLREKLGISDGDVE
                     ETEDAPKSLFDEE"
BASE COUNT         1124 a          718 c          521 g         1362 t
ORIGIN      
        1 tactttgtct gaaaacttca atatgttagt attaagacat caaaataaat aggagttgta
       61 aataaataca tgtctattaa tattgatcca gaaaaatttg cagaattagt ggttatgtct
      121 aacccatcaa aattcgaaga tgcagaggat atagctaaag aaagtttaaa gctttacatt
      181 aatgcatatc gtttagcaga gagatattca acaattgcaa caaattgtta tgatactgca
      241 gaagttataa aagaattaaa gaaaacagat ttacaattaa aataactcaa cttagaaata
      301 aatcctaatt aatattgcag aagagaagat gtgttacatc ttctcttctt ttttattgga
      361 tagaaaagca atcatcttta taggtttaca taatataaat atagtaatta cgaagcctaa
      421 aacattttta ttaaaagcaa taacttctta tttaatataa ggtccgtctt actttcaaat
      481 aactactgtt taatatagtt attaccgata aagtgctgtc taactagatt aagactacaa
      541 cttaaaaaag ctccctcact aatttgtgag ggagacaaaa attatttcgc atattctact
      601 gctctagtct ctcgaacaac tgtcaccttg atatgaccag gatattgtaa ttcatcttca
      661 atctgatttt taatatctct agccaatcga taagatttta aatcatcaat ttcttcagga
      721 gatacaatca ctcggatttc tctacctgcc tgaatcgcaa atgctttttc tacaccatca
      781 taactttctg ataacgtttc taaacgttct aatcgacgaa tataattctc taatgtttct
      841 tttcttgcac ctggacgagc cgcagacaat gcatctgcag cagcaacaag gatagatata
      901 atagatgtag gttcaacatc accatgatga gaatggattg cattaataac tgtttcattt
      961 tcaccatatt ttttcgctaa ttctacaccg atttcaacat gactaccttc tacttcatga
     1021 tcaattgctt taccaacatc atgtaaaagt ccagctcgtt tcgctaatgt ctcatcttcg
     1081 cctagctcag cagctaacat actagcaaga tgcgcaactt caattgaatg tttaagtaca
     1141 ttttgaccgt aactcgtacg atagtttaaa cgccctacaa tttttactaa gtcaggatgc
     1201 atattatgtg cgttcacttc aaatgtagct tgttcacctg cttctctaat aatatcgtct
     1261 acttcttttc tagctttttc gaccatatct tcaattctac ctggatgaat acgtccatca
     1321 gatactaagt taacaagtgc tgttctagca atttctcttc ttattggatc aaaaccagat
     1381 aatataaccg cttctggtgt gtcatcaata attaaatcaa tgccagttaa agtttcaagt
     1441 gtgcggatgt ttcgtccttc tcgtccaatg attcgacctt tcatctcatc attaggtaag
     1501 ttaactactg ataccgttga ttcacttgtg tgatctgctg ctaatctttg tactgctgta
     1561 gctaataatt cttttgctgt tttatcaact ttttctttag cttctttttc tttttcttta
     1621 acaagtactg caatatcttg tgacagttct tcctctactc tttgaagttg ctcattaata
     1681 gcttcttctt gagtgagacc ggagatgcgt tctaattctt gttcatgctt cattattaac
     1741 gtttgaacac tactctcttt tgcatctact tgttgttgtt tttcttcaat ttttgattct
     1801 ttttgctcta aaatctcatc ttttttatct aatagatcag atttgcgctc taagttttct
     1861 tctttttgaa gaagtcgggt ttcttgtctt tgaagttcgc tacgtctttc tcgtagttct
     1921 gcttcagttt gttctcttag gatttggttt tcttcttttg cctcaagtaa tttctctttt
     1981 ttgatattgt cagcttcttt atgtgcttga tttacaatat cttcggcagt ttgtctagct
     2041 tgtgattgct tttgaagcaa caaatttcgg gcaacaacat accctccaac aactcctaga
     2101 atgatcccca gcaaaatgag taggaggctt aataaattca cacaaacacc tccttttcta
     2161 gggtttgctc tttatatgaa attcaatatg actttatgaa attatatata aatcatacaa
     2221 attaattgta cggtttatag gagataagtg tcaagttatc aatttgcact tttgcactat
     2281 attgaatgct atttctacta ttttctaaag ttttgaagct cgattataaa actattgata
     2341 atgtaataat aaataaaata aaaaacctta tagtctcgat ttgtagtgta tcccataaag
     2401 ttagatatta ttctagcttt atgagatgaa tatcactttc aatcaatcct ataaggatat
     2461 ttgctaagtt taactataga tataaatttg tgtactattc ttcgtcaaat aatgactttg
     2521 gtgcatcttc tgtttcttca acatcaccat cagatatacc taatttttct ctcaatttac
     2581 gatcaatttc ttctttaatt tgtggatttt ctttcaagta cattttaaca ttttccttac
     2641 cttgacccat tcgttcgcca ttgtaagaat accatgctcc tgatttatca acgatgtcgt
     2701 tttcaacacc taaatcaata agttcaccct ctttagaaat accttgtcca tacataatat
     2761 caacttcagc tactctaaat ggtggtgcca ctttattttt aacgacttta attttagttc
     2821 tattacctac aatttcttgt ccttgtttaa gctgttctgc acgacgtact tctagtctta
     2881 ctgaactata gaattttaat gcacgtccac ctggtgtagt ctctggatta ccgaacataa
     2941 caccaacttt ttcacgaatt tggttgatga aaatagcagt tgtatttgat ttagaaatag
     3001 cacctgaaag tttacgtaac gcttgtgaca ttaaacgagc ttgtaaacca acgtgagtgt
     3061 ctcccatttc tccttcaatt tcagctttag gtgttaaagc agcaactgag tctacaacta
     3121 caatatcaac tgcaccactt ctaacaaatg cttcggcgat ttcaagacct tgttcaccat
     3181 gatccggttg cgataaatat aaattatcga tatctacgcc taatgcttga gcatattctg
     3241 gatctaaagc atgttcagca tcgataaatg ctgccacccc gccattactt tgtacttcag
     3301 caatagcgtg aagcgctact gttgtcttac cagaactttc aggaccataa atttcaataa
     3361 ttcgtccttt aggatagcca cctacaccta gcgcattatc taatgtaact gaaccagttg
     3421 atgtagttga aactcggcga cctatattgt cacccaactt cattacggca cctttaccga
     3481 aagatttctc catattttta attactgtat ctaaagcttt ttgacgatcg ttatccaaag
     3541 cgagacctcc taattgaaat tgctatcttt taattatctt accaatacta tacaaggatt
     3601 caaaataaaa tacaagtgtt ttgcgaatat ttgttcgtat aaaatcttga aatcaaaacg
     3661 caaaaacgaa caaacgtgct tatattttta tcgataatac aattaatttg ttgttctaag
     3721 ccaat
//
LOCUS       DAKTIO010000212         3718 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.217, whole genome shotgun sequence.
ACCESSION   DAKTIO010000212 DAKTIO010000000
VERSION     DAKTIO010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.217"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            364..1845
                     /locus_tag="O0293_002520"
     CDS             364..1845
                     /locus_tag="O0293_002520"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HCY0281378.1"
                     /translation="MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVG
                     YTVDSHKFCQNVADQGCKLVVVNKEQSLPANVTQVVVPDTLRVASILAHTLYDYPSHQ
                     LVTFGVTGTNGKTSIATMIHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLT
                     KKIKEAVDAGAESMTLEVSSHGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHA
                     KSLLFSQLGEDLSKEKYVVLNNDDSFSEYLRTVTPYEVFSYGIDEEAQFMAKNIQESL
                     QGVSFDFVTPFGTYPVKSPYVGKFNISNIMAAMIAVWSKGTSLETIIKAVENLEPVEG
                     RLEVLDPSLPIDLIIDYAHTADGMNKLIDAVQPFVKQKLIFLVGMAGERDLTKTPEMG
                     RVACRADYVIFTPDNPANDDPKMLTAELAKGATHQNYIEFDDRAEGIKHAIDIAEPGD
                     TVVLASKGREPYQIMPGHIKVPHRDDLIGLEAAYKKFGGGPVD"
     gene            1829..2089
                     /locus_tag="O0293_002521"
     CDS             1829..2089
                     /locus_tag="O0293_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueH family protein"
                     /protein_id="HCY0281379.1"
                     /translation="MALLINKRFIDEGKTIDVYLFEALNNQIIIAIPDWFWSYQMAMT
                     LDEETCFEAILMQLFVFKEEEEAESIASQLTDWIETYKKEKD"
     gene            2089..3651
                     /locus_tag="O0293_002522"
     CDS             2089..3651
                     /locus_tag="O0293_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCY0281380.1"
                     /translation="MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVK
                     GKKTGKFATSDWMKVEQERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMA
                     VDSAVMVIDCAKGIEPQTLKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLN
                     IETYPMNWPIGMGQSFFGIIDRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSDFE
                     QAIEELMLVEEAGEAFDNDALLSGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQ
                     TKEDVEVSPFDDSFSGFIFKIQANMDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQ
                     KITRSTSFMADDKETVNHAVAGDIIGLYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEI
                     FMKVSAKNVMKQKHFHKGIEQLVQEGAIQYYKTLHTNQIILGAVGQLQFEVFEHRMKN
                     EYNVDVVMEPVGRKIARWIENEDQITDKMNTSRSILVKDRYDDLVFLFENEFATRWFE
                     EKFPEIKLYSLL"
BASE COUNT         1332 a          504 c          744 g         1138 t
ORIGIN      
        1 ataaagacgc aaagtcgtta catttatagc aattttaatc tatagatgaa ttgatacaaa
       61 ataaaacgtt attttataaa gcaatttatt gttctatgtt ttatttgtat atttaaaatt
      121 atccagtata caattatagc atatttttgg aaacaattat gatattatac catgttacaa
      181 gatggtttta ataatttaag atgagccata attgtaaaac taattcataa taccgtatgt
      241 tttattttta atagtagaaa ttagaaaatg ctgattagta ggatataaca gtgaaattat
      301 aaatttatta acatcaacaa aacgtgtata ataaacatat tgtagaaaaa ggagcggttc
      361 agtttggatg caagtacgtt gtttaagaaa gtaaaagtaa agcgtgtatt gggttcttta
      421 gaacaacaaa tagatgatat cactactgat tcacgtacag cgagagaagg tagcattttt
      481 gtcgcttcag ttggatatac tgtagacagt cataagttct gtcaaaatgt agctgatcaa
      541 gggtgtaagt tggtagtggt caataaagaa caatcattac cagctaacgt aacacaagtg
      601 gttgtgccgg acacattaag agtagctagt attctagcac acacattata tgattatccg
      661 agtcatcagt tagtgacatt tggtgtaacg ggtacaaatg gtaaaacttc tattgcgacg
      721 atgattcatt taattcaaag aaagttacaa aaaaatagtg catatttagg aactaatggt
      781 ttccaaatta atgaaacaaa gacaaaaggt gcaaatacga caccagaaac agtttcttta
      841 actaagaaaa ttaaagaagc agttgatgca ggcgctgaat ctatgacatt agaagtatca
      901 agccatggct tagtattagg acgactgcga ggcgttgaat ttgacgttgc aatattttca
      961 aatttaacac aagaccattt agattttcat ggcacaatgg aagcatacgg acacgcgaag
     1021 tctttattgt ttagtcaatt aggtgaagat ttgtcgaaag aaaagtatgt cgtgttaaac
     1081 aatgacgatt cattttctga gtatttaaga acagtgacgc cttatgaagt atttagttat
     1141 ggaattgatg aggaagccca atttatggct aaaaatattc aagaatcttt acaaggtgtc
     1201 agctttgatt ttgtaacgcc ttttggaact tacccagtaa aatcgcctta tgttggtaag
     1261 tttaatattt ctaatattat ggcggcaatg attgcggtgt ggagtaaagg tacatcttta
     1321 gaaacgatta ttaaagctgt tgaaaattta gaacctgttg aagggcgatt agaagtttta
     1381 gatccttcgt tacctattga tttaattatc gattatgcac atacagctga tggtatgaac
     1441 aaattaatcg atgcagtaca gccttttgta aagcaaaagt tgatattttt agttggtatg
     1501 gcaggcgaac gtgatttaac taaaacgcct gaaatggggc gagttgcctg tcgtgcagat
     1561 tatgtcattt tcacaccgga taatccggca aatgatgacc cgaaaatgtt aacggcagaa
     1621 ttagccaaag gtgcaacaca tcaaaactat attgaatttg atgatcgtgc agaagggata
     1681 aaacatgcaa ttgacatagc tgagcctggg gatactgtcg ttttagcatc aaaaggaaga
     1741 gaaccatatc aaatcatgcc agggcatatt aaggtgccac atcgagatga tttaattggc
     1801 cttgaagcag cttacaaaaa gttcggtggt ggccctgttg attaataaaa gatttattga
     1861 tgaaggtaaa actattgatg tttatttatt cgaagcatta aataaccaga taatcattgc
     1921 tataccagat tggttttggt catatcagat ggcaatgaca ttagatgaag aaacttgttt
     1981 tgaagcaata ctcatgcaat tgtttgtttt taaagaagag gaagaggcag aatcgattgc
     2041 atcacaacta acagattgga tagaaacata taaaaaggag aaagactaat gaacttaaag
     2101 caagaagttg agtctagaaa gacttttgcg attatttcac atcccgatgc agggaaaaca
     2161 acgttaactg aaaaactatt gtacttcagt ggtgctattc gtgaagcggg tacagttaaa
     2221 gggaagaaga ctggtaaatt tgcgacaagt gactggatga aagttgaaca agagcgtggt
     2281 atttctgtaa ctagttcagt aatgcaattt gattacgatg attataaaat caatatctta
     2341 gatacaccag gacatgaaga cttttcagaa gatacgtata gaacattaat ggcagttgac
     2401 agtgctgtca tggtcataga ctgtgcaaaa ggtattgaac cacaaacatt gaagttattt
     2461 aaagtttgta aaatgcgtgg tattccaatc tttacattca ttaataaatt agaccgagta
     2521 ggtaaagaac catttgaatt attagatgaa atcgaagaga cattaaatat tgaaacatac
     2581 cctatgaatt ggccaattgg tatgggacaa agtttctttg gcatcattga tagaaagtct
     2641 aaaacaattg aaccatttag agatgaagaa aatatattac atttgaatga tgattttgag
     2701 ttggaagaag atcatgcaat tacaaatgat agtgattttg aacaagcgat tgaagaatta
     2761 atgttggttg aagaagcggg tgaagccttt gataatgacg cgctgttgag tggagactta
     2821 acacctgtat ttttcggttc agctttagct aactttggtg tacaaaattt cttaaatgca
     2881 tatgttgatt ttgcgccaat gccaaatgcg agacaaacaa aagaagacgt tgaagtaagc
     2941 ccgtttgatg attcattttc aggatttatc tttaaaattc aagccaacat ggaccctaaa
     3001 caccgtgata gaattgcctt tatgcgtgtc gttagtggtg catttgaacg tggtatggat
     3061 gttactttgc aacgtactaa taaaaagcaa aagatcacac gttcaacgtc atttatggca
     3121 gacgataaag aaactgtgaa tcatgctgta gcaggcgata tcattggact atatgatact
     3181 ggtaattatc aaattggaga tactttagtt ggtggaaaac aaacctacag tttccaagat
     3241 ttaccacaat ttacgccaga aatttttatg aaagtttctg ctaaaaacgt catgaaacag
     3301 aagcatttcc ataaaggtat tgaacaatta gtacaagaag gtgcgattca atactataaa
     3361 acattacaca caaaccaaat tattttaggt gctgttggtc agttacaatt tgaagttttc
     3421 gaacatagaa tgaaaaacga atataatgtt gatgttgtta tggagccagt aggccgtaaa
     3481 attgcacgtt ggattgaaaa tgaagaccaa attacagata agatgaacac atcaagatcg
     3541 attttagtga aagatagata tgacgattta gtattcttat ttgaaaatga atttgcaaca
     3601 agatggtttg aagagaaatt ccctgaaatt aaattgtata gtttacttta acagctcaat
     3661 tgtataatcg aatttgttac attaaaaata attgtttcgt tgaagaaaaa taaattgt
//
LOCUS       DAKTIO010000213         3675 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.218, whole genome shotgun sequence.
ACCESSION   DAKTIO010000213 DAKTIO010000000
VERSION     DAKTIO010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.218"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            86..1612
                     /gene="spa"
                     /locus_tag="O0293_002523"
     CDS             86..1612
                     /gene="spa"
                     /locus_tag="O0293_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal protein A"
                     /protein_id="HCY0281381.1"
                     /translation="MKKKNIYSIRKLGVGIASVTLGTLLISGGVTPAANAAQHDEAQQ
                     NAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNNFNK
                     DQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNNFNK
                     EQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLSEAKKLNESQAPKADNKFNK
                     EQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNK
                     EQQNAFYEILHLPNLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPKEEDNNKP
                     GKEDNNKPGKEDNNKPGKEDNNKPGKEDGNKPGKEDNKKPGKEDGNKPGKEDNKKPGK
                     EDGNKPGKEDGNKPGKEDGNGVHVVKPGDTVNDIAKANGTTADKIAADNKLADKNMIK
                     PGQELVVDKKQPANHADANKAQALPETGEENPFIGTTVFGGLSLALGAALLAGRRREL
                     "
     gene            complement(1941..3533)
                     /locus_tag="O0293_002524"
     CDS             complement(1941..3533)
                     /locus_tag="O0293_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCY0281382.1"
                     /translation="MTLLTVNPFDNVGLSALVAAVPIILFLLCLTVFKMKGIYAALTT
                     LVVTLIVALFVFELPARVSAGAITEGVVAGIFPIGYIVLMAVWLYKVSIKTGQFSIIQ
                     DSIASISVDQRIQLLLIGFCFNAFLEGAAGFGVPIAICAVLLIQLGFEPLKAAMLCLI
                     ANGAAGAFGAIGLPVSIIDTFNLSGGVTTLDVARYSALTLPILNFIIPFVLVFIVDGM
                     KGIKEILPVILTVSGTYTGLQLLLTIFHGPELADIIPSLATMVVLAFVCRKFKPKNIF
                     RLEASEHKIQKRTPKEIVFAWSPFVILTAFVLVWSAPFFKKLFQPGGALESLVIKLPI
                     PNTVSDLSPKGIALRLDLIGATGTAILLTVIITILITKLKWKSAGALLVEAIKELWLP
                     ILTISAILAIAKVMTYGGLTVAIGQGIAKAGAIFPLFSPVLGWIGVFMTGSVVNNNTL
                     FAPIQATVAQQISTSGSLLVAANTAGGVAAKLISPQSIAIATAAVKKVGEESALLKMT
                     LKYSIIFVAFICVWTFILTLIF"
BASE COUNT         1389 a          712 c          570 g         1004 t
ORIGIN      
        1 ttaattataa atatagattt tagtattgca atacataatt cgttatatta tgatgacttt
       61 acaaatacat acagggggta ttaatttgaa aaagaaaaac atttattcaa ttcgtaaact
      121 aggtgtaggt attgcatctg taactttagg tacattactt atatctggtg gcgtaacacc
      181 tgctgcaaat gctgcgcaac acgatgaagc tcaacaaaat gctttttatc aagtcttaaa
      241 tatgcctaac ttaaatgctg atcaacgcaa tggttttatc caaagcctta aagatgatcc
      301 aagccaaagt gctaacgttt taggtgaagc tcaaaaactt aatgactctc aagctccaaa
      361 agctgatgcg caacaaaata acttcaacaa agatcaacaa agcgccttct atgaaatctt
      421 gaacatgcct aacttaaacg aagcgcaacg taacggcttc attcaaagtc ttaaagacga
      481 cccaagccaa agcactaacg ttttaggtga agctaaaaaa ttaaacgaat ctcaagcacc
      541 gaaagctgat aacaatttca acaaagaaca acaaaatgct ttctatgaaa tcttgaatat
      601 gcctaactta aacgaagaac aacgcaatgg tttcatccaa agcttaaaag atgacccaag
      661 ccaaagtgct aacctattgt cagaagctaa aaagttaaat gaatctcaag caccgaaagc
      721 ggataacaaa ttcaacaaag aacaacaaaa tgctttctat gaaatcttac atttacctaa
      781 cttaaacgaa gaacaacgca atggtttcat ccaaagccta aaagatgacc caagccaaag
      841 cgctaacctt ttagcagaag ctaaaaagct aaatgatgct caagcaccaa aagctgacaa
      901 caaattcaac aaagaacaac aaaatgcttt ctatgaaatt ttacatttac ctaacttaac
      961 tgaagaacaa cgtaacggct tcatccaaag ccttaaagac gatccttcag tgagcaaaga
     1021 aattttagca gaagctaaaa agctaaacga tgctcaagca ccaaaagagg aagacaataa
     1081 caagcctggc aaagaagaca ataacaagcc tggcaaagaa gacaacaaca agcctggtaa
     1141 agaagacaac aacaagcctg gcaaagaaga cggcaacaag cctggtaaag aagacaacaa
     1201 aaaacctggt aaagaagatg gcaacaagcc tggtaaagaa gacaacaaaa aacctggtaa
     1261 agaagacggc aacaagcctg gcaaagaaga tggcaacaaa cctggtaaag aagatggtaa
     1321 cggagtacat gtcgttaaac ctggtgatac agtaaatgac attgcaaaag caaacggcac
     1381 tactgctgac aaaattgctg cagataacaa attagctgat aaaaacatga tcaaacctgg
     1441 tcaagaactt gttgttgata agaagcaacc agcaaaccat gcagatgcta acaaagctca
     1501 agcattacca gaaactggtg aagaaaatcc attcatcggt acaactgtat ttggtggatt
     1561 atcattagcc ttaggtgcag cgttattagc tggacgtcgt cgcgaactat aaaaacaaac
     1621 aatacacaac gatagatatc attttatcca aaccaatttt aacttatata cgttgattaa
     1681 cacattctta tttgaaatga taagaatcat ctaaatgcac gagcaacatc ttttgttgct
     1741 cagtgcattt tttattttac ttacttttct aaacaacttc tgaaacgcct caacactttc
     1801 tactctgatt acatatatga catttttagg cattaaaaaa tcgaactaga caagatgctc
     1861 attgcatttc gtactagttc gattcatgaa taattagatt taaaatgtca tttgaatcca
     1921 agtgacaaca ttatttatat ttagaatatt aacgttagta taaacgtcca aacacaaata
     1981 aaagcaacaa atataatact gtattttaac gtcattttta ataatgcaga ttcttcacca
     2041 acttttttaa cagctgcagt cgcaatggca attgattgtg gtgaaataag tttcgctgct
     2101 acaccacctg cagtgttagc tgccacaagt aatgaaccgc ttgttgaaat ttgttgtgcc
     2161 actgtcgctt gaataggtgc gaataaagta ttgttattta caactgaacc agtcataaac
     2221 acaccaatcc aacctaatac tggagagaat aatgggaaaa ttgctcccgc tttagcaata
     2281 ccttgtccaa ttgctacagt caaaccaccg tatgtcataa ctttagcaat agctaggata
     2341 gctgaaattg taaggatcgg taaccataat tctttaattg cttcgaccaa taaagcacct
     2401 gcacttttcc attttaactt cgtaattaaa attgtaataa ttactgttaa taaaatcgct
     2461 gtcccagttg caccaattaa atcgagacgc aacgcaattc ctttaggcga taaatcactc
     2521 acagtatttg gaattggcaa ttttattact aaactttcaa gtgcacctcc aggttggaat
     2581 aattttttga agaatggtgc actccatact aatacaaagg cagttaaaat tacgaacgga
     2641 ctccaagcaa agacaatttc tttaggcgtt cgtttttgaa ttttatgttc agacgcttcc
     2701 aatctgaaaa tgtttttcgg tttaaattta cgacaaacaa atgctaacac caccattgtt
     2761 gctagtgatg gaataatgtc tgctagttct ggaccatgga atattgttaa taataattgt
     2821 aatccagtat atgtaccact cactgttaaa atgacaggta aaatttcttt aatacctttc
     2881 ataccatcta caatgaatac taaaacaaat ggaataataa agtttaaaat tggaagtgtt
     2941 aatgctgagt atctcgcaac atctaatgtt gtaacgcctc cacttaagtt aaacgtatca
     3001 ataatactaa ctggtaaacc aattgcacca aaggcacccg ccgcaccatt agcaattaaa
     3061 cataacatcg ctgcttttaa tggttcaaat ccaagttgaa ttaataatac tgcacaaatc
     3121 gcaattggca caccaaatcc tgctgcacct tctaaaaatg cgttgaaaca aaatccaatt
     3181 aataatagtt ggattctttg gtccactgaa atacttgcaa tactatcttg aataatagaa
     3241 aattgtcctg ttttaataga aactttatat aaccaaactg ccattaaaac gatatatcct
     3301 attgggaaaa taccggcaac aacgccttct gtaatcgcac ctgctgatac acgcgctggt
     3361 aattcaaata caaataaagc cacaatcaat gtaacaacca aagttgtcaa tgctgcataa
     3421 atgcctttca ttttaaaaac ggttaagcat aataaaaata aaataatagg tactgctgca
     3481 actaaggctg ataatccgac attatcgaat ggatttacag taagtagtgt cataatgact
     3541 ccctctcttt atataaaata tttatcattc tgattaatct acaacctatt tcaacttata
     3601 ttttgcgatg atcacatatt taaaatgtaa cactcctata tgtgacaggc aatcgaattt
     3661 ttacaaaaag ttcac
//
LOCUS       DAKTIO010000214         3607 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.219, whole genome shotgun sequence.
ACCESSION   DAKTIO010000214 DAKTIO010000000
VERSION     DAKTIO010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.219"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(73..1209)
                     /locus_tag="O0293_002525"
     CDS             complement(73..1209)
                     /locus_tag="O0293_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HCY0281383.1"
                     /translation="MTENKSFKESHPLDDFISDKELSNTTIQKEKLTIEQQKQVDTIS
                     KQINPLDNEGLLAFGSDLQKQMSQFSHQMLDEVQSKDVGPIGDTLSDLMSKLKSVNPN
                     ELNTDKPSMLKRIFSRAKSSINEIFSRMQSVSAQVDRITIQLQKHQTHLTRDIELLDT
                     LYDKNKQYFDDLSLHIIAAQQKKLQLENEKLPQLQQQAQQSTNQMDIQQVADMQQFID
                     RLDKRIYDLQLSRQIALQTAPQIRMIQNVNQALAEKIQSSILTSIPLWKNQMAIALTL
                     MRQRNAVAAQRAVTDTTNDLLTANAEMLKQNAIETATENERGIVDLDTLKRTQRNIIE
                     TIEETLIIQQHGREERQLAEKELQQLEQDLKSHLVNIKGPNKQS"
     gene            complement(1241..1870)
                     /locus_tag="O0293_002526"
     CDS             complement(1241..1870)
                     /locus_tag="O0293_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HCY0281384.1"
                     /translation="MTVRYNISHIFGVLVGIPVAFLTSIFGMIALDVSFLIDMSIGIV
                     GFLMTYLPIQKLTSRKYLNEIGLTRKDYRYIRNQLNHTHQKLRGILKTYVNIRSIKDF
                     RQINDIYQISRSIYTTVRQRPASFYKVEGFFYSHIDNALNLVDAYTRLAKMPKKSINE
                     QQKLEQTRITLDEVKRTLIADLKRLNEDDYERLDIEMELNKLHQKHHQD"
     gene            complement(1889..2158)
                     /locus_tag="O0293_002527"
     CDS             complement(1889..2158)
                     /locus_tag="O0293_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCY0281385.1"
                     /translation="MRHIHLQVFGRVQGVGFRYFTQRIAMNYNIVGTVQNVDDYVEIY
                     AQGDDADIERFIQGVIEGASPASNVTSHQLEELELNQKLSDFRSI"
     gene            2321..2629
                     /locus_tag="O0293_002528"
     CDS             2321..2629
                     /locus_tag="O0293_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HCY0281386.1"
                     /translation="MWTVTKIRADYEGWWLFSDWPENIVEKYQYQDFDDMFKHYQQLI
                     NQCKVQFDNYVTGKYNIYAFYNNCDMNYCEDCEEDLQIFYSFIVLQNNEVYYKLPIID
                     "
     gene            2800..3000
                     /gene="cspA"
                     /locus_tag="O0293_002529"
     CDS             2800..3000
                     /gene="cspA"
                     /locus_tag="O0293_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspA"
                     /protein_id="HCY0281387.1"
                     /translation="MKQGTVKWFNAEKGFGFIEVEGENDVFVHFSAINQDGYKSLEEG
                     QAVEFEVVEGDRGPQAANVVKL"
     gene            3197..3598
                     /gene="msa"
                     /locus_tag="O0293_002530"
     CDS             3197..3598
                     /gene="msa"
                     /locus_tag="O0293_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarA expression modulator protein Msa"
                     /protein_id="HCY0281388.1"
                     /translation="MKYLILSLVANLLVFGVLSAIGLNINILAAMMIVLVIPIMISGI
                     LFFKTNIDKTYIFFNIIFIDFYYYIYNVHLMTLPKFNNYIKAEMMELEDIDVLITSKD
                     FGFDEILFYTLYLLLILIVLYYLKKQVKHKI"
BASE COUNT         1129 a          519 c          563 g         1396 t
ORIGIN      
        1 ttttttacat tgcctaagaa ctttaaggaa cttaatgtcc caagctcttt tttgttatat
       61 ctaattcgta atttatgatt gtttattcgg tcctttgatg ttcactaaat gtgactttaa
      121 atcttgttct aattgttgta attctttttc agctaattgt cgttcttcgc gaccgtgttg
      181 ttgaataatt aatgtttctt caattgtctc aataatgtta cgctgtgtac gtttcaatgt
      241 atcaagatca acaatgccac gctcattttc tgttgcagtt tcaatcgcat tttgtttcaa
      301 catttcagca tttgctgtta ataaatcatt agttgtatca gtgacagctc gttgtgcagc
      361 aactgcatta cgctgtctca ttaatgtaag cgcaatggcc atttgatttt tccatagtgg
      421 aatacttgtc aaaattgaac tttgtatctt ctcggcaagt gcttgattaa cattttgaat
      481 catacgaatt tgtggcgcag tttgcaaagc tatttgtctt gaaagctgta agtcatatat
      541 gcgtttatct agtctatcta taaattgctg catatctgca acttgttgaa tatccatttg
      601 attagtggat tgctgcgctt gctgttgcaa ttgtggtagc ttttcatttt ctaattgcaa
      661 ctttttttgc tgtgcagcaa tgatatgcaa tgataagtca tcaaagtatt gtttgttttt
      721 atcatatagc gtatctaata attcaatatc tcttgttaaa tgtgtttgat gtttctgcag
      781 ttgaatcgtt atgcgatcga cttgagcact aactgattgc attcttgaaa agatttcatt
      841 gatagacgac tttgctctgc taaaaattct ttttaacata gatggtttat cagtatttaa
      901 ctcatttgga ttaactgact ttagttttga cattagatct gacaaagtat ctccaatagg
      961 accaacatct ttactttgta cttcatccaa catttgatgt gaaaattgag acatttgttt
     1021 ctgtaaatca gaaccaaacg ctaataaacc ttcattgtct aaagggttaa tttgtttact
     1081 gattgtgtct acctgttttt gttgttcaat tgttaacttt tctttttgaa tagtagtatt
     1141 cgataattct ttatcgctta taaaatcatc tagtggatgg ctttctttga aacttttatt
     1201 ttcagtcatc aatcatctct ccaatagttg ctattttaca tcaatcttga tgatgttttt
     1261 gatgtaactt atttaattcc atttcaatat ctaaacgttc ataatcatct tcgttgagac
     1321 gctttaaatc agcgattaat gttcgtttga cctcatccaa agtaattcgt gtttgttcta
     1381 acttttgctg ttcattaatt gattttttgg gcatttttgc tagacgtgta tatgcatcaa
     1441 ccaaatttaa agcattatca atatgagaat aaaaaaagcc ttcaacttta taaaatgatg
     1501 caggtctctg tctaactgtc gtataaatag aacgtgaaat ttggtatata tcattaatct
     1561 gcctaaaatc tttaattgat cttatattga catacgtttt taaaatacct ctaagttttt
     1621 ggtgtgtatg atttaactga tttcgaatat agcgatagtc ttttctagtc aaaccaattt
     1681 cgtttaaata tttgcgtgaa gtgagttttt gtatcggtag gtatgtcatt aaaaagccaa
     1741 caataccaat agacatatca attaaaaaag atacatcaag tgcaatcatc ccaaatatgc
     1801 ttgttaaaaa cgctacagga attcccacta acaccccaaa tatatgagaa atattatatc
     1861 tcactgtcat cttcctttat ttagcatttt atattgatcg aaaatccgat aatttttgat
     1921 ttagttctaa ctcttcaagt tgatggcttg ttacatttga tgctggtgag gcaccttcaa
     1981 ttacaccttg aataaatctc tctatatctg cgtcatcccc ttgtgcatat atctctacat
     2041 agtcatctac attttgaaca gtaccgacaa tgttatagtt cattgcaatg cgttgtgtaa
     2101 aatatctaaa tccgacgcct tgaacgcgtc cgaatacttg taaatgtata tgtctcattt
     2161 ttaccacctc ataatgttat tatacgtagt tttacttaaa aaaactaata attactatag
     2221 ttactacttt gtttgtttca agtcgtcaaa cttgattttc agaggataaa ggtataaaaa
     2281 taagtataga gtttttgaag tatggaaggg gtctttaata atgtggacag ttaccaaaat
     2341 tagagccgat tatgagggat ggtggttatt cagtgactgg ccagaaaaca ttgttgaaaa
     2401 atatcaatat caagattttg atgacatgtt taagcactat caacaattga ttaatcaatg
     2461 taaagttcag ttcgataact atgtcacagg caaatataat atttatgcat tttataataa
     2521 ttgtgacatg aactattgcg aagattgtga agaggattta caaatatttt atagtttcat
     2581 tgttttacaa aataatgaag tatattataa actaccaata attgattaaa tgaatatttt
     2641 ttattattgt attgcaattt ggtaataact gcactataat acaagttaag cagatgatta
     2701 ttccatattg caaagaatat ttgagtgaaa tttgcatgta ttttttgtaa tatgcgaata
     2761 agcatattga atgaatttta gtcttggagg tttcagatta tgaaacaagg tacagttaaa
     2821 tggtttaacg ctgaaaaagg attcggcttt atcgaagttg aaggagaaaa tgacgtattc
     2881 gtacattttt cagcaattaa ccaagatggt tacaaatcat tagaagaagg tcaagctgtt
     2941 gagtttgaag tagttgaagg cgaccgcggt ccacaagctg caaacgttgt taaactataa
     3001 ttcttagatt tgaatcattg attttaacta acaccttacc aagaagtaag gtgtttttat
     3061 atgcttatta caaatactta acgcgatgag tttctcaacc aaattgagaa ctcttttttt
     3121 atatcaccaa cattgtttgc aatagaaaaa tatatttata taatataatt atggtgtaca
     3181 acgacgaggt gattttatga aatatcttat ccttagttta gttgctaatt tattagtttt
     3241 cggtgtatta tctgcaattg gattaaatat taatatttta gcagcaatga tgattgtatt
     3301 agtgattccg ataatgattt ctgggatact atttttcaaa actaatattg ataaaacata
     3361 catatttttt aacatcatat ttatcgactt ttattactat atttacaatg tacatttaat
     3421 gactttgcca aagtttaata attatattaa agcagagatg atggaattag aagatattga
     3481 tgtattaatt acaagtaaag attttggatt tgatgaaatt ctgttttata cattatattt
     3541 gttgttaatt cttatcgttt tatattattt gaaaaagcaa gtaaagcata aaatctaatt
     3601 tcatgat
//
LOCUS       DAKTIO010000215         3574 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.220, whole genome shotgun sequence.
ACCESSION   DAKTIO010000215 DAKTIO010000000
VERSION     DAKTIO010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.220"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..297
                     /locus_tag="O0293_002531"
     CDS             <1..297
                     /locus_tag="O0293_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCY0281389.1"
                     /translation="PQKVVTDQAPSTKAAMAKIIKAFKLKPDCHCTSKYLNNLIEQDH
                     RHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIMLAS"
     gene            355..732
                     /locus_tag="O0293_002532"
                     /pseudo
     CDS             355..732
                     /locus_tag="O0293_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            729..1610
                     /locus_tag="O0293_002533"
                     /pseudo
     CDS             729..1610
                     /locus_tag="O0293_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1726..2655
                     /locus_tag="O0293_002534"
     CDS             1726..2655
                     /locus_tag="O0293_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0281390.1"
                     /translation="MKNLNNIIEINNLTKQYGSQIAVNNLSITVESGQIYGFLGRNGA
                     GKTTTIRMLLGLIKPTKGNIKIFEKEFESNKTEILKNIGAMVETPGFYSNLTAKENLT
                     INAKILGIQKENAIEEVLEIVGLASEKVKLFKNYSLGMKQRLGIARALLHNPELLILD
                     EPTNGLDPIGIKEIRKLIKDLAQSRNITILISSHILSEIEQLVDKVGIIHNGVLLEEI
                     MYEDLKKKNRQFISISVSDEEKVACLLETNFGIYDYKIKENGEFKIYSHIDRQQEINR
                     LLVLNNIDVFQMRVIEDNLETYFEDLVGGGTIG"
     gene            2648..3385
                     /locus_tag="O0293_002535"
     CDS             2648..3385
                     /locus_tag="O0293_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0281391.1"
                     /translation="MDNLLRTEFIKLKRSYMFLISILGTCVAPFMVVVASFISMRTKV
                     PTPNIVFKQLFEEVSLYHMLIIGPPLYGVIIAYLFGREYIEDTLNNLLIIPISRESFL
                     VSKIILLFLWIQTLSVIAWLLTLMLGMLLHFQGLSQSLIIDSFFTFVIGGVLLWILSP
                     PIIILSIYFKNYVPTIVLTIIITLINLMTANSEHRGLFPWAAVGDISRGTLPNTYPFL
                     LSLIIIIVTSIFSVIILFSYFKKTDIS"
     gene            3399..>3574
                     /locus_tag="O0293_002536"
     CDS             3399..>3574
                     /locus_tag="O0293_002536"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499250.1"
                     /note="BacA; phosphatase activity in Escherichia coli not
                     kinase; involved in bacitracin resistance as bacitracin
                     supposedly sequesters undecaprenyl disphosphate which
                     reduces the pool of lipid carrier available to the cell;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase"
                     /protein_id="HCY0281392.1"
                     /translation="MGFIEQIKAFILGVVEGLTEFAPVSSTGHMIIVDDMWLNTADDL
                     GSASATTFKVVIQLG"
BASE COUNT         1307 a          491 c          632 g         1144 t
ORIGIN      
        1 cctcaaaagg tagttacaga tcaggcacct tcaacgaagg cagcaatggc taaaataatt
       61 aaagctttta aacttaaacc tgactgtcat tgtacatcga aatatctgaa taacctcatt
      121 gagcaagatc accgtcatat taaagtaaga aagacaagat atcaaagtat caatacggca
      181 aagaatactt taaaaggtat tgaatgtatt tacgctctat ataaaaagaa ccgtaggtct
      241 cttcagatct acggattttc cccatgccac gaaattagca tcatgctagc aagttaaacg
      301 aacactgaca tgatatatta gtgattagct atatttttta ctttgcaaca gaaccagcca
      361 tttaatatga ctgttacaat tactaagatt caagcactat tacgccgcac gtatgatttc
      421 accactcaag ttaataattt aacagtagat gattgcgagt tactacttga tgcagcaaag
      481 cttaaattta atgaagaaac catagacctg acacacactg aattgcaaat catgcatagc
      541 ttattccaga acaagggcaa attcgtcagt cgtaatgcgt taatcgaaaa atgctgggaa
      601 tcagaacact tcattgatga caatacgctt gctgtcaaca ttacacgttt acgcaaaaag
      661 ctacaaaaac taggacttac agattttatc gaaacgaaga aaaatgtcgg atatcgtgta
      721 ggtgacgtat gaatttcttg aaatcaatcc aacgtgaaat ttatattgtc atccttactt
      781 gtgctttatt ctactttata ttattcatgt ataatgtgcc gatgcaagca tatatgattg
      841 gtctaggtat tgtattgttt gtttcaatga tttactgggg tatccattac atgaacttta
      901 aaaaagaata aacactacag gaacagaacg aagatttaag aatcgaaaac cataaacttc
      961 gtaatgactt tattaactac caaagtgagt tagagtcata ttttttaatg tgggtgcatc
     1021 aaatcaagac accgattaca gcatcaaagt tactgcttga agatcctgat gaacacgtga
     1081 tttctaacgt gcgtaatgaa gtcttacaaa ttgataatta cacgaatctt gcactgaact
     1141 atttgaaact catgaaccaa aagacataca tggtctttat ggaagtgagt gtagatgaat
     1201 taattcgacc actgattaaa cgttatgcga tacagtttat tcatcatcaa acaaagttgc
     1261 attatgagaa aagtgaagcg gttatattaa cagatgccaa atgggcatca attatgattg
     1321 agcaaatatt aaacaatgca ttaaaatatg cacgtggcaa agatgtgtgg attacgtttg
     1381 acgaaaatac aaagaaatta tcaatccgag ataacggtat cggcattaat gcgagtgatt
     1441 taccgaaaat ctttgataaa ggtttctcgg gctataacgg tagaataagt gagaaatcaa
     1501 gcggtattgg tttatatatt gtcagcacaa tctcaaagcg cttaggccat aaagtaagtg
     1561 ctacatctac attaaacgat ggttcaatat tcaccattca atttaaataa ctccaatctt
     1621 tcatttttgt aagattagcg gctgtttttg taagaataga taaaggcagc cttttctaat
     1681 cgcgcgtaag ataaaggtaa caacaaacta agaaaggtgc cagaggtgaa aaatttgaat
     1741 aatataattg aaataaataa cttaacaaag caatatggaa gtcagatagc agttaataat
     1801 ttgagtataa ctgttgaaag tggacaaatt tatggttttt taggaagaaa tggtgcaggc
     1861 aaaacaacta ctataagaat gcttttaggc ttaattaaac ctacgaaagg taatattaaa
     1921 atatttgaaa aagagttcga aagtaataaa actgaaattt taaaaaatat tggtgctatg
     1981 gttgaaacgc caggttttta tagtaatttg acagctaaag agaacttaac tataaatgct
     2041 aaaatattag gtatacaaaa agaaaatgca attgaagaag tgttagaaat agtaggacta
     2101 gcttctgaaa aagtaaaatt gtttaaaaat tattctttag gtatgaagca acgtttaggg
     2161 attgcgagag ctttattgca caatcccgaa ttattgatac ttgatgagcc gacgaatgga
     2221 ttagatccta ttggcataaa agaaattaga aagttgatta aggatctggc tcaatctaga
     2281 aatataacca ttttaatttc aagtcatata ttgtcagaaa tagagcagtt agtagacaag
     2341 gtgggtatta ttcataacgg agttttgtta gaagaaatta tgtatgaaga tttaaaaaaa
     2401 aagaacagac aatttataag tatatcagta tcagatgaag aaaaagttgc atgtttgttg
     2461 gaaactaact tcggaattta tgactataaa attaaagaaa atggcgaatt taaaatttat
     2521 tcacatattg atagacaaca agaaattaat agattattag tattaaacaa tattgatgtt
     2581 ttccagatga gagtgataga agataatcta gaaacttatt tcgaagactt agtaggaggt
     2641 ggcacaattg gataatctac tccgtacaga atttattaag ttaaaaagat catatatgtt
     2701 tctaataagt attttaggta catgcgtagc accttttatg gtggttgtag cttcttttat
     2761 tagtatgcgg acgaaagtac cgactcctaa tattgtattt aaacagttat ttgaagaagt
     2821 cagtttatat catatgctaa ttattggacc tcctctatat ggggttataa tagcctattt
     2881 gtttggaaga gaatatatag aagatacttt aaataactta ttaattatac ctatatctag
     2941 agaaagtttt ttagtaagta aaataatatt attattcttg tggattcaaa cactatctgt
     3001 tattgcatgg ttgctaactt taatgctagg tatgctattg cattttcaag ggttaagcca
     3061 gagtttaata attgattcat tctttacatt tgttattggc ggagtgttat tatggatatt
     3121 atctccacca ataattattt tgtcgatata ttttaaaaat tatgttccca caattgtttt
     3181 aaccataata attactttga taaatttaat gactgctaat tctgaacata gaggattatt
     3241 tccttgggct gcagttggag acatatctag aggaacatta cctaatactt atccatttct
     3301 attatcttta attatcataa ttgttacatc tatattcagt gtaattatat tattctcata
     3361 ctttaaaaaa actgatataa gttaaaagga ggagaaaaat gggttttata gagcagatta
     3421 aagcatttat tttaggtgta gttgaagggt tgacagaatt tgctccagta tcatcaacag
     3481 gtcatatgat tattgttgat gatatgtggt taaacactgc tgacgattta ggaagtgcgt
     3541 ctgcaactac ttttaaggtg gtaatacagt tagg
//
LOCUS       DAKTIO010000216         3456 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.221, whole genome shotgun sequence.
ACCESSION   DAKTIO010000216 DAKTIO010000000
VERSION     DAKTIO010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.221"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(348..1184)
                     /locus_tag="O0293_002537"
     CDS             complement(348..1184)
                     /locus_tag="O0293_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCY0281393.1"
                     /translation="MDLASKYFNGVNWRYIDHSSGLEPMQSFAFDDTFCESVGKDISD
                     NVVRTWIHQHTVILGIHDSRLPFLKDGIDYLTNEIGYNAIVRNSGGLGVVLDQGVLNI
                     SLMFKGQTETTIDEAFTVMYLLISKMFENENVDIDTMEIEHSYCPGKFDLSIDGKKFA
                     GISQRRVRGGIAVQIYLCVEGSGSERALMMQTFYEHALKGEVTKFKYPEIEPSCMASL
                     ETLLNKTITVQDVMFLLLYAIKDLGGVLNMTPITQEEWQRYDTYFDKMIERNKKMIDQ
                     MQ"
     gene            complement(1187..2173)
                     /gene="pta"
                     /locus_tag="O0293_002538"
     CDS             complement(1187..2173)
                     /gene="pta"
                     /locus_tag="O0293_002538"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCY0281394.1"
                     /translation="MADLLNVLKDKLSGKNVKIVLPEGEDERVLTAATQLQATDYVTP
                     IVLGDETKVQSLAQKLDLDISNIELINPATSELKAELVQSFVERRKGKATEEQAQELL
                     NNVNYFGTMLVYAGKADGLVSGAAHSTGDTVRPALQIIKTKPGVSRTSGIFFMIKGDE
                     QYIFGDCAINPELDSQGLAEIAVESAKSALSFGMDPKVAMLSFSTKGSAKSDDVTKVQ
                     EAVKLAQQKAEEEKLEAIIDGEFQFDAAIVPGVAEKKAPGAKLQGDANVFVFPSLEAG
                     NIGYKIAQRLGGYDAVGPVLQGLNSPVNDLSRGCSIEDVYNLSIITAAQALQ"
     gene            2341..3093
                     /locus_tag="O0293_002539"
     CDS             2341..3093
                     /locus_tag="O0293_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent peroxidase"
                     /protein_id="HCY0281395.1"
                     /translation="MSQAAETLDGWYSLHLFYAVDWASLRIVPKDERDALVTEFQSFL
                     ENTATVRSSKSGDQAIYNITGQKADLLLWFLRPEMKSLNHIENEFNKLRIADFLIPTY
                     SYVSVIELSNYLAGKSDEDPYENPHIKARLYPELPHSDYICFYPMNKRRNETYNWYML
                     TMEERQKLMYDHGMIGRKYAGKIKQFITGSVGFDDFEWGVTLFSDDVLQFKKIVYEMR
                     FDETTARYGEFGSFFVGHIINTNEFDQFFAIS"
BASE COUNT         1114 a          625 c          499 g         1218 t
ORIGIN      
        1 ctttatcttt ttataaaata gccctacaat ttataatttt ccaccctaac tataatacta
       61 caaataataa ttggaatata tagatttact actaaagtat tagaacattt caatagaagg
      121 tcgtttcttt catagtcata cgcattatat ataccctatt ctcaatctat ttaatacgta
      181 aaacatgaaa ttttcttatt aaatttatta tttccatcat atcattactt ttaatttaat
      241 gatgttcaat tcaaatatta ggtcaataac atatttatgc tttttatgga tactttcaaa
      301 aataacagcc ccaaacgata acttgaaagg ggctgttaaa tatttaacta ttgcatttga
      361 tctatcattt tcttgtttct ttcaatcatt ttatcaaaat acgtatcgta tctttgccat
      421 tcttcttgag taattggcgt catatttaat acaccgccaa gatctttgat tgcatataat
      481 agtaaaaaca ttacatcttg aacagtaatc gttttgttaa gcaatgtctc taatgaggcc
      541 atacaagatg gttcaatttc aggatattta aatttagtca cttcaccttt taaagcatgt
      601 tcataaaatg tttgcatcat caatgcacgt tctgaaccag agccttcaac acaaagataa
      661 atttgtacag caataccgcc tctaactctt cgttgcgata tgcctgcaaa tttcttacca
      721 tcgatactta agtcaaattt tcctgggcaa taagaatgtt caatttccat cgtatcaata
      781 tcaacattct cattttcgaa cattttgcta attaagaggt acatcacagt aaacgcttca
      841 tcaatcgttg tttctgtttg tcctttgaac atcagcgata tatttaatac accttgatct
      901 agaacgacac ctaagccacc agaatttcta acaatggcat tataaccaat ctcattcgtt
      961 aaataatcaa tgccatcttt taaaaacggc aatcttgaat catgaatacc aagaataaca
     1021 gtatgttgat gaatccaagt acgcacaaca ttatctgata tatctttgcc cacactttcg
     1081 caaaatgtat catcgaatgc gaaagattgc ataggttcta atccagaaga atgatcgata
     1141 tatcgccagt tgacgccatt aaaatattta ctcgctaaat ccatcgttat tgtaaggctt
     1201 gcgctgctgt aataattgaa agattgtata catcttcaat tgagcagcca cgtgataagt
     1261 catttactgg agaatttaaa ccttgtaata ctggaccaac tgcatcatat ccacctaaac
     1321 gttgtgcaat tttgtaacca atattaccag cttctaaact tgggaataca aagacatttg
     1381 catcaccttg taatttagca cctggcgctt ttttctcagc aacacctggt acaatcgcag
     1441 catcaaattg gaattcgcca tcaatgattg cttctaattt ttcttcttca gctttttgtt
     1501 gtgctaattt gacagcttct tgaacttttg tcacgtcgtc tgatttagca gacccttttg
     1561 ttgaaaagct taacattgca acttttggat ccatgccaaa gcttaatgct gattttgcac
     1621 tttctactgc aatttctgca agtccttgtg aatcaagttc tggattgatt gcacaatcac
     1681 caaagatgta ttgttcatca cctttaatca taaagaagat acctgatgtt cttgatacac
     1741 ctggtttcgt tttgatgatt tgtaaagctg gacgcacagt gtcgcctgtt gaatgtgctg
     1801 caccactaac taaaccatct gctttaccag cataaacaag cattgtaccg aagtagttca
     1861 cattgtttaa taattcttgt gcttgttctt cagtcgcttt acctttacgt cgttcaacaa
     1921 atgattgaac taattcagct ttcaattcac ttgtcgcagg attaattaat tcaatattag
     1981 aaatatcaag atcaagtttt tgcgctaaag attgaacctt agtctcatca cctaacacga
     2041 ttggtgtaac ataatctgtt gcttgtaatt gtgttgcagc tgttagaaca cgttcgtcct
     2101 ctccttcagg taatacgatt ttaacgtttt taccagaaag tttgtctttt aatacattta
     2161 ataaatcagc cataatgtcc tcctgtaata taaatcttat taatcattca cggtataatt
     2221 atacgccatt ttatttcata atgccacgtc accaatttgt aatcataatt ttatcgttac
     2281 ccctttgctt tatgataaaa tttataaaga actgatgatt tttgaaaagg agcgataaac
     2341 atgagtcaag cagccgaaac attagatggt tggtatagtc tacatttatt ttatgcagtt
     2401 gattgggcat cattacgtat agttccaaag gacgaacgcg atgcacttgt cactgaattt
     2461 caatcatttt tagaaaatac agcaactgta agatcatcaa aatctggtga tcaagctatt
     2521 tataatataa ctggtcaaaa agcagatttg ttattatggt tcttacgtcc tgaaatgaag
     2581 tctttaaatc atattgaaaa tgaatttaac aaattgcgca ttgctgactt cctaatccct
     2641 acatattcat atgtatcagt cattgaattg agcaattatt tagctggtaa atctgatgaa
     2701 gatccttatg agaaccctca tatcaaagca agattatacc cagaattacc acattctgat
     2761 tatatttgtt tctatccaat gaacaaacgt cgtaatgaaa cttataactg gtacatgtta
     2821 actatggaag aacgccaaaa attaatgtat gaccatggta tgattggtag aaaatatgct
     2881 ggcaaaatca aacaatttat tactggttct gtagggtttg atgatttcga atggggcgta
     2941 acattgttct cagatgacgt attacaattc aaaaaaattg tatacgaaat gcgctttgat
     3001 gaaacaacag cacgatacgg tgaattcggt agtttctttg taggacatat tattaacaca
     3061 aacgaattcg atcaattctt tgcgatttct taatacattg gtacgtttat aaattaataa
     3121 aaaaattcca agcttatcgg tttaagcttg gaatttttcg tttatcttca gtatattccc
     3181 gtatacataa gacgtgattt ggtaaatagt tgaaatctgt atgtttaaac ttatatatat
     3241 gtgctaatgt attatcaata acaaagtaca ctttgctcat agcaaagtgt acccgagtag
     3301 tcttccttgg gagaacttta actactatca ctacatataa acgttaacct caatagaaat
     3361 tatacagtcg ctactctata caatttttgt aatggttaac taatattatt ttaacctatt
     3421 tgaaatattt gaaacatatt tttgtcgaat tttttt
//
LOCUS       DAKTIO010000217         3406 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.222, whole genome shotgun sequence.
ACCESSION   DAKTIO010000217 DAKTIO010000000
VERSION     DAKTIO010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.222"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(27..1154)
                     /locus_tag="O0293_002540"
     CDS             complement(27..1154)
                     /locus_tag="O0293_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCY0281396.1"
                     /translation="MKSKYEPLFDKVELPNGVELRNRFVLAPLTHISSNDDGTISDVE
                     LPYIEKRSQDVGITINAASNVSDVGKAFPGQPSIAHDSNIEGLKRLATAMKKNGAKAL
                     VQIHHGGAQALPELTPDGDVVAPSPISLKSFGQKQEHSAREMTNEEIEQAIKDFGEAT
                     RRAIEAGFDGVEIHGANHYLIHQFVSPYYNRRNDVWANQYKFPVAVIEEVLKAKEAYG
                     NKDFIVGYRLSPEEAESPGITMEITEELVNKISHMPIDYIHVSMMDTHATTREGKYAG
                     QERLPLIHKWINGRMPLIGIGSIFTADEALDAVENVGVDLVAIGRELLLDYQFVEKIK
                     DGREDEIINYFDPEREDNHHLTPNLWHQFNEGFYPLPRKDK"
     gene            complement(1516..1893)
                     /locus_tag="O0293_002541"
     CDS             complement(1516..1893)
                     /locus_tag="O0293_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCY0281397.1"
                     /translation="MNNYKIGQHIKVRVTGIQPYGAFVETPNHTEGLIHISEIMDDYV
                     HNLKKFLSEGQIVKAKILSIDDEGKLNLSLKDNDYFKNYERKKEKQSVLDEIRETEKY
                     GFQTLKERLPIWIKQSKRAIRND"
     gene            complement(2309..2902)
                     /locus_tag="O0293_002542"
     CDS             complement(2309..2902)
                     /locus_tag="O0293_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCY0281398.1"
                     /translation="MANYPQLNKEVQQGEIKVVMHTNKGDMTFKLFPNIAPKTVENFV
                     THAKNGYYDGITFHRVINDFMIQGGDPTATGMGGESIYGGAFEDEFSLNAFNLYGALS
                     MANSGPNTNGSQFFIVQMKEVPQNMLSQLADGGWPQPIVDAYGEKGGTPWLDQKHTVF
                     GQIIDGETTLEDIANTKVGPQDKPLHDVVIESIDVEE"
     gene            2966..3349
                     /locus_tag="O0293_002543"
     CDS             2966..3349
                     /locus_tag="O0293_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase-associated lipoprotein B"
                     /protein_id="HCY0281399.1"
                     /translation="MKLYRFSHKTGSYGVTIVEENGDDVLVKVEQVIKHPKQGDLHNP
                     TETEGVFFHDRKALSHFEKRYTKRSQLRDFNVDVLRYEDSLQQAITDLETKLKAQQTK
                     HAEMSLASLARLKEDYSLQYKENFK"
BASE COUNT         1068 a          627 c          486 g         1225 t
ORIGIN      
        1 tatcattagt ggtaacagtt aaacacttat ttatctttac gtggtaatgg ataaaaccct
       61 tcattaaatt gatgccataa attaggagtt aagtgatgat tatcttctct ctctggatca
      121 aagtaattaa taatttcatc ttcccgtcca tctttaattt tttcaacaaa ttgataatcc
      181 agtagtagct ctctaccaat ggctactaag tcaacaccaa cattttcaac tgcatctaaa
      241 gcttcgtcag ctgtgaaaat tgaaccaata ccgataagtg gcatacgacc atttatccat
      301 ttgtgaatta aaggcagtct ttcttgtcca gcgtatttac cttcacgtgt cgttgcatgc
      361 gtatccatca ttgaaacatg aatatagtcg attggcatat ggctaatttt attaacgagt
      421 tcctctgtaa tttccattgt gattcctgga gactccgctt cctctggaga taatctgtat
      481 ccaactataa agtctttatt gccatacgct tctttcgctt taagtacttc ttcaatcaca
      541 gcgaccggga atttatattg atttgcccat acatcatttc ttctattata gtatggtgat
      601 acaaattgat gaattaagta atgattcgcg ccatgtattt caacaccatc aaaccctgct
      661 tcaattgcac gtcgcgttgc ttcaccaaaa tccttgattg cttgttcaat ctcttcattc
      721 gtcatttctc tagcactatg ttcttgtttc tgaccaaaac tttttaaaga aattggactt
      781 ggtgctacga cgtctccatc aggtgttaat tcaggcaatg cttgtgcacc gccatgatgt
      841 atttgtacga gtgctttggc accgtttttc ttcattgctg tagctaatcg ttttagtcct
      901 tcaatattac tgtcatgcgc gattgatggc tgtcctggaa atgcttttcc gacatcactc
      961 acattactcg cagcattaat tgtaatacca acatcttgtg aacgcttttc aatataagga
     1021 agttctacat ctgaaatagt accatcatca tttgaagaaa tatgtgttaa aggggctaac
     1081 acaaatcgat ttctcaactc tactccattt ggtaattcta ctttatcaaa caatggttcg
     1141 tatttacttt tcattactta attcctcctt aaaattattg agttgactat aacatgtaat
     1201 tttttcattc tccaattatc tgtttatata gttgcaattc gtttaatact ttaagttgta
     1261 gttctcattt ctaaaataaa taaaaaaaac aaatccacat acaatattgc aaatcaacga
     1321 ttctatctaa caaagataat ggatatgtca ccttaaacca tttgtacgtg taaatagaat
     1381 caatcatatt acgttatcgt agacaaaaag agattagtat ctaataaaat ttaatagata
     1441 ctaatctctt ttatagtcta agaaactttc atttcagacc ctttgtatga ttttcggtac
     1501 gatttatctg ttcctttagt cgtttcgaat tgctcgcttt gactgtttta tccagattgg
     1561 taagcgttct ttaagtgttt gaaacccata tttttctgtt tctctgattt catctaatac
     1621 tgattgtttt tccttcttac gctcataatt tttgaagtaa tcattatcct ttaatgatag
     1681 attaagcttt ccttcatcat ctatagacaa aattttagct ttaacaattt ggccttctga
     1741 tagaaatttc ttcaaattat gaacgtagtc atccataatt tctgatatat gaatcagtcc
     1801 ttcagtatga ttaggggtct caacaaacgc accgtatggt tgaataccag ttacacgcac
     1861 cttgatatgt tggccaattt tgtagttatt caacttaata accccttatt tcgtttactt
     1921 aacactatta tcatagcaca aatcgaaatg gtggactaat taaaacatca aatcatacga
     1981 atattatttt ataaatgaat aagaattcta caaacttgga tttacaaata aaattagcaa
     2041 caaattccta tctaaatcaa tatgatatcc agctactttg ttttcaattt atttactatt
     2101 tgaatagcaa ttctctgtaa aaacataaaa agcaacctca actattatct ttaaaataca
     2161 catctattta aaaactgtgc tttcttgata aatatattga gattgctcat catattagtt
     2221 tatttatttt cgttggaata tatactaatc cattcataaa ttagctttat ccgagtttaa
     2281 aatgttggta gttaattatg tttagatatt attcttcaac atcaatagat tcaattacaa
     2341 catcatgaag tggtttatct tgtggtccca cttttgtatt tgcaatatct tctaatgtag
     2401 tttcaccatc aatgatttga ccgaatactg tatgtttttg atctaaccat ggtgtaccac
     2461 ccttttcgcc atatgcatca acgattggtt gaggccagcc accatctgca agttgactta
     2521 acatattttg aggtacttct ttcatttgaa caatgaaaaa ttgtgaacca ttagtattag
     2581 gtcctgagtt agccattgat aatgcgccat ataagttaaa tgcatttaat gaaaattcat
     2641 cttcaaaagc accgccataa atactttcgc cacccatacc agtagctgtt ggatcgccac
     2701 cttgaatcat gaagtcatta atgacacggt ggaatgtgat tccatcataa taaccatttt
     2761 ttgcatgtgt cacaaaattt tcaactgttt ttggtgcaat atttggaaat aatttgaatg
     2821 tcatgtcacc tttatttgtg tgcataacca ctttgatttc accttgttgt acttctttgt
     2881 ttaactgtgg atagttagcc atttatattc tccattcatg ttatgataca tatatattaa
     2941 catactttta agggaggaaa atagcatgaa gctatatcga ttttcacaca aaacaggtag
     3001 ctatggtgtc acgattgttg aagaaaatgg agacgatgtc cttgttaaag ttgaacaagt
     3061 tatcaaacac ccgaaacaag gtgatttaca taaccctact gaaactgaag gtgtattttt
     3121 ccatgaccgc aaagcattaa gccattttga aaaacgctat acgaaacgtt cacaattacg
     3181 cgacttcaat gttgatgtat tgcgctatga agattcactt caacaagcaa ttacagattt
     3241 agaaactaaa cttaaagctc agcaaacaaa acatgctgaa atgtctttag ctagtttagc
     3301 acgcttaaaa gaagattatt cacttcaata caaagaaaat tttaaataaa aaatgacgtg
     3361 tttcttctta tttaaatact tctgaaaata aagcacaaca aggagt
//
LOCUS       DAKTIO010000218         3406 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.223, whole genome shotgun sequence.
ACCESSION   DAKTIO010000218 DAKTIO010000000
VERSION     DAKTIO010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.223"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..382
                     /locus_tag="O0293_002544"
     CDS             <1..382
                     /locus_tag="O0293_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YisL family protein"
                     /protein_id="HCY0281400.1"
                     /translation="LHILSWVLAIILFIATYLNISKNQGRSPFFKPLHMILRLFMLLT
                     LISGFWILIQSFMNGGANHMLLTLKMLCGVAVVGLMEVSIAKRKRHEQSHTMFWITIA
                     LIIITMVLGVILPLGPISKLFGIG"
     gene            complement(553..1869)
                     /locus_tag="O0293_002545"
     CDS             complement(553..1869)
                     /locus_tag="O0293_002545"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HCY0281401.1"
                     /translation="MPKIVVVGAVAGGATCASQIRRLDKESDIIIFEKDRDMSFANCA
                     LPYVIGEVVEDRRYALAYTPEKFYDRKQITVKTYHEVIAINDERQTVSVLNRKTNEQF
                     EESYDKLILSPGASANSLGFESDITFTLRNLEDTDAIDQFIKANQVDKVLVVGAGYVS
                     LEVLENLYERGLHPTLIHRSDKINKLMDADMNQPILDELDKREIPYRLNEEINAINGN
                     EITFKSGKVEHYDMIIEGVGTHPNSKFIESSNIKLDRKGFIPVNDKFETNVPNIYAIG
                     DIATSHYRHVDLPASVPLAWGAHRAASIVAEQIAGNDTIEFKGFLGNNIVKFFDYTFA
                     SVGVKPNELKQFDYKMVEVTQGAHANYYPGNSPLHLRVYYDTSNRQILRAAAVGKEGA
                     DKRIDVLSMAMMNQLTVDELTEFEVAYAPPYSHPKDLINMIGYKAK"
     gene            complement(1921..2745)
                     /locus_tag="O0293_002546"
     CDS             complement(1921..2745)
                     /locus_tag="O0293_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCY0281402.1"
                     /translation="MQPHLICLDLDGTLLNDNKEISSYTKQVLNELQQRGHQIMIATG
                     RPYRASQMYYHELNLTTPIVNFNGAYVHHPKDKNFKTCHEILDLGIAQNIIQGLQQYQ
                     VSNIIAEVKDYVFINNHDPRLFEGFSMGNPRIQTGNLLVHLKESPTSILIEAEESKIP
                     EIKNMLTHFYADHIEHRRWGAPFPVIEIVKLGINKARGIEQVRQFLNIDRNNIIAFGD
                     EDNDIEMIEYARHGVAMENGLQELKDVANNITFNNNEDGIGRYLNDFFNLNIRYYC"
     gene            2859..3167
                     /locus_tag="O0293_002547"
     CDS             2859..3167
                     /locus_tag="O0293_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HCY0281403.1"
                     /translation="MEEALKDSILGALEMVIDPELGIDIVNLGLVYKVNVDDEGVCTV
                     DMTLTSMGCPMGPQIIDQVKTVLAEIPEIQDTEVNIVWSPPWTKDMMSRYAKIALGVS
                     "
BASE COUNT         1060 a          555 c          576 g         1215 t
ORIGIN      
        1 tttacatata ttaagttggg tattagcgat tattttattt atcgctacat acttaaacat
       61 ttcaaaaaat caaggcagat caccattttt caaaccgttg cacatgattt tacgcttatt
      121 tatgctgttg acgttaattt caggattttg gatattaatt cagtcattta tgaatggcgg
      181 ggcaaatcat atgttgctta cattgaaaat gctgtgtggt gttgcagtag ttggattgat
      241 ggaagtgtcg attgctaaaa gaaagagaca tgaacaaagt cacacaatgt tttggataac
      301 aattgcatta attatcatca caatggtatt aggtgtcatt ctaccgttag ggcctatatc
      361 aaaattattc ggtattggct aattgataag acatatagta tatgacataa ggcatggaca
      421 attattagtc caagacctta tgtctttttt gattctaaaa atgttaaaca taaaaaatgg
      481 actggatagt agtaccagtc caacataatt ctaacctaga caattaagat tagattgaat
      541 cgcatgattc atttatttag ctttgtaacc aatcatattg attaaatctt tagggtggct
      601 atatggtggt gcataagcca cttcaaactc agttaactca tctacagtta gctggttcat
      661 cattgccatc gatagtacat caatacgttt atctgcacct tcttttccta ctgcagctgc
      721 tcttaaaatc tgacggtttg aagtgtcata atatactctt aagtgtaaag gggaatttcc
      781 tgggtaataa ttcgcgtgtg caccttgagt gacttctacc attttatagt caaattgctt
      841 tagttcgttt ggtttaacgc cgacactcgc aaatgtataa tcaaagaact tcacaatatt
      901 gttgcctaag aagcctttga attcaatagt gtcatttcca gcaatttgtt cggcaacaat
      961 acttgctgca cggtgagcgc cccaagctaa aggaacacta gccggtagat cgacatgtcg
     1021 ataatgtgat gttgcaatat cgcctattgc ataaatgttt ggaacatttg tttcaaattt
     1081 atcgtttacc ggtatgaaac cttttcgatc aagtttgata tttgaacttt cgataaattt
     1141 tgaattgggg tgagtaccga caccttcaat aatcatatcg taatgttcaa cttttcctga
     1201 tttaaatgta atttcatttc cattgatagc attaatttcc tcatttaaac ggtatggaat
     1261 ctcccgctta tctaattcat caagtatagg ttgattcatg tcggcatcca ttaatttatt
     1321 tatcttatca gatcgatgaa ttaaagtagg gtgtaaacca cgttcataaa gattttcaag
     1381 aacttctaat gaaacataac ctgcacctac aaccaatact ttatcaactt gatttgcttt
     1441 gatgaattga tcgatagcat cagtgtcttc taaatttcta agtgtaaatg taatatcact
     1501 ttcaaagcca aggctatttg cacttgcacc agggcttaaa atgagtttat cgtaagattc
     1561 ttcaaattgt tcgtttgtct ttctatttaa tacagataca gtttgtcttt catcattgat
     1621 tgcaataact tcatgataag tttttactgt aatttgcttt ctatcataaa atttttcagg
     1681 tgtatacgct aaagcatatc ttctatcttc aacaacttcg ccaatgacat aaggcaatgc
     1741 acaattagca aagctcatat cacgatcttt ttcaaaaata ataatgtcac tttctttatc
     1801 taaacgtcga atttggctgg cacatgttgc accgccagcg actgctccga ctacgactat
     1861 tttgggcatc gtaacttacc tcctattgtt ttaaaatatt ataaaatgat tagttataaa
     1921 ttaacagtaa tatctaatat ttaaattaaa gaaatcattc aaatatcgac caatgccatc
     1981 ttcattattg ttgaatgtaa tattgttcgc tacatcttta agttcttgca aaccattttc
     2041 catagcaaca ccgtgacgcg cgtactcaat catttcaata tcattatctt catcaccgaa
     2101 tgcaataata ttatttcggt caatatttaa aaattgtcta acttgctcaa tgcctcttgc
     2161 tttattaata ccaagtttta caatttcaat gacagggaat ggtgcgcccc agcgtcgatg
     2221 ctcaatatga tcggcataaa aatgagtaag catatttttg atttcaggta ttttactttc
     2281 ttcggcttca attaaaattg aggtagggga ttctttcaag tggacaagta aattaccagt
     2341 ttgaattctt ggattaccca ttgaaaaacc ttcaaataat cttggatcat gattgttaat
     2401 gaaaacataa tctttcactt ctgctataat attcgatact tgatattgtt gtaatccttg
     2461 aataatgttt tgtgcgatgc ctaaatctaa aatttcatgg caagttttga agtttttatc
     2521 tttagggtga tgtacgtaag cgccattaaa attaacaatt ggtgtcgtta aatttaattc
     2581 atgataatac atttgacttg cacgataagg tctgccagtc gcaatcataa tttggtgtcc
     2641 acgttgttgt aattcattta atacttgttt agtatatgat gaaatttctt tgttatcgtt
     2701 taataatgtt ccgtctaagt ctagacatat taaatgtggt tgcatatctc tctcctcata
     2761 agttgcttag ttcatataga tacatcatag cattttattg gcgttttgtc ggatatttga
     2821 tatattatga ataatatgac attaaagagg tgatatggat ggaagaggca ttgaaagata
     2881 gtatcttagg tgcattagaa atggtaattg accctgaatt aggaattgat atcgttaatt
     2941 tgggtttagt atacaaagtg aatgttgatg atgaaggcgt atgtacagtt gatatgactt
     3001 taacatcaat gggatgtcca atgggacctc aaattattga tcaagttaaa acagtattag
     3061 cagagattcc tgaaattcag gatactgaag tgaatatcgt atggagtcca ccttggacaa
     3121 aagatatgat gtcacgttac gctaagattg cacttggtgt gagctaacat aaacttgcat
     3181 tgcctgtaga atttcttttc gaaattctct gtgtttgggc ccgcagtcaa tataacattg
     3241 tagagtataa ttcattgtga ataagctccc ttcaaagtag acattgaaaa atgaaaattt
     3301 tgaagggagc cttatttatg ttctagtttc tgattttatt ttgaaaattt atatctaact
     3361 tgtttcaaaa aatatgaatt aagacagtga cttgcacttc caagag
//
LOCUS       DAKTIO010000219         3306 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.224, whole genome shotgun sequence.
ACCESSION   DAKTIO010000219 DAKTIO010000000
VERSION     DAKTIO010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.224"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..1188
                     /locus_tag="O0293_002548"
     CDS             <1..1188
                     /locus_tag="O0293_002548"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCY0281404.1"
                     /translation="NNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVACET
                     TVTTGMALIAGEISTTTYVDIPKVVRETIKEIGYTRAKYGYDYETMAILTAIDEQSPD
                     IAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKRLS
                     DVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHAEDVTLEQIQEDIKAHV
                     IYPTVPENLINEQTKFYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAEPVSIAIDTFGTGKVSE
                     GQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEELKD
                     AVK"
     gene            1313..2221
                     /locus_tag="O0293_002549"
     CDS             1313..2221
                     /locus_tag="O0293_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HCY0281405.1"
                     /translation="MDLSSPIVIGLIIAVVIALIFFVLFLVALGSKKKVKRQTEEKYE
                     QQKQNIKKSHEEALEKERIQNKKTITKQQEDYNHMVSTKDREIDALKLFSKNHSEYVT
                     DMRLIGIRERLVKEKRIRPEDMHIMANIFLPKDGFNNIERISHLVLTRTGLYIIDSQL
                     LKGHVYNGISGGQFKDLPPMEQVFDTLDLDKSRPQTIVMDQNDDKRSLSFVNYSDQIE
                     AIKQLAEDLQKELGAKYTPTSILYFNFKNEGDVTISNYNQNSAVKVLVGAEQLDEFFN
                     KFVFHGRIQYNVEDLQQMMDKIESFN"
     gene            2433..3266
                     /locus_tag="O0293_002550"
     CDS             2433..3266
                     /locus_tag="O0293_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCY0281406.1"
                     /translation="MEVKTFYNGNTMPQIGLGTFRVENDENCMESVKYAIEQGYRSID
                     TAKVYGNEEQVGAGIRAGLESTGIAREDLFITSKLYFEDFGRENVAAAYEASLSRLGL
                     KYLDLYLVHWPGTNEAVMVDTWKGMEDLYKNNNVKNIGVSNFEPEHLEALLAQVSIKP
                     VINQVEYHPYLTQHKLKLYLAAQRIVMESWSPLMNAQILNDETIKDIAQELGKSPAQV
                     VLRWNVQHAVVTIPKSVTPNRISENFQIFDFELSDEQMTRIDGLNQDKRIGPDPKKFE
                     G"
BASE COUNT         1203 a          461 c          647 g          995 t
ORIGIN      
        1 aataacaaac gattatttac ttcagagtct gttacagaag gacacccaga taaaatcgct
       61 gaccaagtgt cagatgcaat attagatgct attttaaaag acgatccaaa tgcacgtgta
      121 gcttgtgaaa caactgttac aacaggtatg gcattgatcg ccggcgaaat ttctacaaca
      181 acatatgttg atattccgaa agttgttaga gaaacaatta aagaaattgg atacacaaga
      241 gcaaaatatg gttatgatta tgaaacaatg gcaattttaa ctgcgattga tgaacaatca
      301 cctgacattg cacaaggcgt ggataaagca ttagagtatc gtgataaaga tagcgaagaa
      361 gaaattgaag caacaggtgc aggtgaccaa ggtttaatgt ttggttacgc aacgaatgaa
      421 acagagacgt atatgcctct agcaatttat ttatcacatc aattggcaaa acgtttatca
      481 gatgtgcgta aagatggtac gttaaattat ttacgaccag acggtaaagt tcaggtcact
      541 gttgaatatg atgaaaatga taacccagta cgtattgata cgattgtcgt atcaacacaa
      601 catgctgagg atgtgacact tgaacaaatt caagaagaca ttaaagcaca tgtcatttat
      661 ccaactgttc cagaaaactt gattaatgaa caaactaaat tctacatcaa tcctacagga
      721 cgctttgtaa tcggtggacc tcaaggtgat gcaggattaa caggacgtaa aatcattgtt
      781 gatacgtacg gtggctatgc acgtcatggt ggcggatgct tcagtggtaa ggatcctaca
      841 aaagtagacc gttcagctgc atacgctgca cgttatgtgg ctaaaaatat tgttgcagca
      901 ggtttagcag atcaatgtga agtacaattg gcatatgcga ttggtgttgc agaaccagtg
      961 tcaattgcaa ttgatacatt tggaacaggt aaagtttctg aagggcaact tgtcgaagca
     1021 gttagaaaac actttgacct aagacctgca ggtattatta aaatgcttga tttgaaacaa
     1081 ccgatttaca aacaaactgc tgcttatggt cattttggac gtacagatgt cttattccca
     1141 tgggaaaaat tagacaaagt ggaagaatta aaagacgcag taaaataata agatgaattg
     1201 aatatagcta aataaaagat tttcggtata taaatgataa tatgtatcga aaatctttag
     1261 cttttctatt ataattaaag ttattcatat attaaaaagg agcgttgttg tgatggattt
     1321 atcttcaccg atagtcattg gactcattat tgcagtagtc attgcattaa tcttttttgt
     1381 ccttttttta gttgccttag gtagtaagaa aaaagttaag cgacaaacag aagaaaagta
     1441 tgaacaacaa aaacaaaaca ttaaaaagtc tcatgaagaa gctttagaga aagagcgtat
     1501 tcaaaataaa aagacaatta caaaacaaca agaagattat aatcacatgg ttagtacaaa
     1561 agatcgtgag attgacgcat taaaattgtt ctcgaaaaat catagtgaat atgtaacgga
     1621 catgagatta attggtattc gtgaaagatt agttaaagaa aaacgaattc gaccagaaga
     1681 tatgcacatt atggcaaata tctttttacc aaaggatggc tttaataata ttgaacgaat
     1741 tagtcattta gtattaacaa gaactggtct ttatattatt gattctcaat tgttaaaagg
     1801 tcatgtttat aatggtatta gtggcggtca atttaaagat ttaccaccaa tggaacaagt
     1861 atttgataca ttggatttag ataaatctag accgcaaact attgtgatgg atcaaaatga
     1921 tgacaaacgt tcattatcat ttgtaaatta ttcagatcaa attgaggcga ttaaacaatt
     1981 ggctgaagac ttacaaaaag aattaggtgc caaatataca ccaacttcaa ttttatactt
     2041 taattttaaa aatgaaggcg atgtaacgat ttcaaattat aatcaaaata gtgctgtcaa
     2101 agtgcttgtc ggtgctgaac aattagacga gttctttaac aaatttgtat tccatggtcg
     2161 tattcaatac aacgtggaag acttacaaca aatgatggat aaaattgaat cattcaatta
     2221 aatgttaggc tgaagtgatg actatatgtt gttacttcgg cttttttagt ttgtcataaa
     2281 ttaaacagaa ttacttttac aatttgatta atatttgaga gatattacta gaattttagt
     2341 ggttgattgc tctaagtgtt ttttctcatg tattcaactc agatttgtta tgataacagg
     2401 gtataataca tcaaaaaggt gaggcgatat ttatggaggt taaaacattt tataatggaa
     2461 acacgatgcc acaaattggt ttagggacat ttcgtgtgga aaatgatgaa aattgcatgg
     2521 aaagtgttaa gtatgccatt gaacaaggat atcgtagtat tgataccgca aaagtttatg
     2581 ggaatgaaga acaagtaggt gctggcattc gtgccggatt ggagtcaact ggtattgcta
     2641 gagaagactt atttattact tcaaaattat attttgagga tttcggtcgt gaaaatgtag
     2701 cagctgctta cgaagctagt ttatctagat taggtttaaa atacttagat ttgtatctag
     2761 tacattggcc aggtacgaac gaagccgtaa tggttgatac atggaaaggt atggaagatt
     2821 tatataaaaa taataatgtt aaaaatatag gtgtcagcaa ttttgaacct gaacatttgg
     2881 aagctttgtt ggcacaagtg tcaattaaac ctgtaattaa tcaagttgaa tatcatccat
     2941 atttaaccca acataaattg aaattatatt tggcagcaca acgtatcgtg atggaatctt
     3001 ggtcaccatt gatgaatgca caaattttaa atgatgagac aattaaagac attgctcaag
     3061 aattaggaaa gtcacctgcc caagttgttt taagatggaa tgtgcagcat gctgtggtta
     3121 caatccctaa atcggtgaca ccaaacagaa tctctgaaaa tttccaaata tttgatttcg
     3181 aattatcaga tgaacaaatg acgcgaattg atggtttaaa tcaagataag agaattggac
     3241 ctgatccaaa aaaatttgaa ggctagatta aaatcgctca actgatgaaa aggttagatg
     3301 aattgt
//
LOCUS       DAKTIO010000220         3294 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.226, whole genome shotgun sequence.
ACCESSION   DAKTIO010000220 DAKTIO010000000
VERSION     DAKTIO010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.226"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            134..850
                     /locus_tag="O0293_002551"
     CDS             134..850
                     /locus_tag="O0293_002551"
                     /EC_number="2.7.7.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribitol-5-phosphate cytidylyltransferase"
                     /protein_id="HCY0281407.1"
                     /translation="MKYAGILAGGIGSRMGNVPLPKQFLDLDNKPILIHTLEKFILIN
                     DFEKIIIATPQQWMTHTKDTLRKFKISDERIEVIQGGSDRNDTIMNIVKHIESTNGIN
                     DDDVIVTHDAVRPFLTHRIIKENIQAALEYGAVDTVIDAIDTIVTSKDNQTIDAIPVR
                     NEMYQGQTPQSFNINLLKESYAQLSDEQKSILSDACKIIVETNKPVRLVKGELYNIKV
                     TTPYDLKVANAIIRGGIADD"
     gene            843..1868
                     /locus_tag="O0293_002552"
     CDS             843..1868
                     /locus_tag="O0293_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCY0281408.1"
                     /translation="MINQVYQLVAPRQFEVTYNNVDIYSDYVIVRPLYMSICAADQRY
                     YTGSRDENVLSQKLPMSLIHEGVGEVVFDSKGVFNKGTKVVMVPNTPTEKDDVIAENY
                     LKSSYFRSSGHDGFMQDFVLLNHDRAVPLPDDIDLSIISYTELVTVSLHAIRRFEKKS
                     ISNKNTFGIWGDGNLGYITAILLRKLYPESKIYVFGKTDYKLSHFSFVDDVFFINKIP
                     EGLTFDHAFECVGGRGSQSAINQMIDYISPEGSIALLGVSEFPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFQDVVDLYIQYPDIVDKLALLKGQEFEIATINDLTEAFEADLSTSWGK
                     TVLKWIM"
     gene            1890..>3294
                     /locus_tag="O0293_002553"
     CDS             1890..>3294
                     /locus_tag="O0293_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCY0281409.1"
                     /translation="MVKSKIYIDKIYWERVQLFVEGHSENLDLEDSNFVLRNLTETRT
                     MKANDVKIDGNQFVCRFNVAILDNGYYLPEDKYLLVNEQELDYIAQLNPDVINDAYQN
                     LKPEQEEEYNELETQNGKINFLLQTYLKEFRKGGISKKTVYTVTPEISSDVNEFVLDV
                     VVTTPEVKSIYIVRKYKELRKYFRKQSFNTRQFIFKAIFNTTKFFHLKKGNTVLFTSD
                     SRPTMSGNFEYIYNEMLRQNLDKKYDIHTVFKANITDRRGIIDKFRLPYLLGKADYIF
                     VDDFHPLIYTVRFRRSQEVIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKAYV
                     SSETDIPFYAEAFGIKEKNVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKVIL
                     FAPTFRGSGHGTAHYPFFKIDFERLARYCEKNNAVVLFKMHPFVKNRLNIADKHKQYF
                     VDVSDFREVNDILFITDL"
BASE COUNT         1179 a          447 c          614 g         1054 t
ORIGIN      
        1 taagcaaaat atgtataatt ttaagtaata cacagacatt tagagttttg attttaaaat
       61 gagttttcaa ttggaaaatg caacgaaatt taaataatta aatatagata tagttgaatg
      121 gaggaagtat tttatgaaat acgctggtat tctagctgga ggtataggct caagaatggg
      181 taacgtacct ttacctaaac aatttttaga tttagacaac aaaccgattt taatccatac
      241 attagaaaaa tttattttaa ttaatgattt tgaaaaaatt attatcgcga cgccacaaca
      301 atggatgacg catacgaaag atacacttag aaaattcaaa atttctgatg aaagaattga
      361 agtcattcaa ggtggtagcg atcgtaacga tacaattatg aatatcgtta aacatattga
      421 atcaacaaat ggtattaacg atgacgatgt cattgtgaca catgatgcag ttagaccatt
      481 tttaacgcat cgtattatta aagaaaatat tcaagctgct ttagagtacg gtgcagtaga
      541 tacagtgatt gatgctatag atacgattgt tacatctaaa gataatcaaa cgattgatgc
      601 aattccagtg cgtaatgaaa tgtaccaagg tcaaacacct caatcgttta atattaattt
      661 attaaaagaa agctatgcac agttgagtga tgagcaaaag agtattttat ctgatgcttg
      721 taagattatt gtagaaacaa acaaaccggt tcgacttgta aaaggtgagt tatataacat
      781 taaagtaaca acaccttacg atttaaaagt agcgaatgct attattcgag gtggtattgc
      841 cgatgattaa tcaagtatat caattagttg cacctagaca atttgaagtt acgtataaca
      901 acgtagatat ttacagtgac tatgtcattg tacgtccttt atatatgtca atttgtgctg
      961 ccgatcaaag atattatact ggtagccgtg atgagaatgt cttatctcag aaattgccaa
     1021 tgtctttaat tcatgaaggt gttggtgagg tcgtatttga cagtaaaggt gtgtttaata
     1081 aaggtacaaa agtagttatg gtaccgaata cgccgacaga aaaagacgat gtcattgctg
     1141 aaaactattt aaaatcgagc tacttcagat caagtggaca tgatgggttt atgcaagatt
     1201 ttgtgttgct aaatcatgat agagctgtac cactacctga tgatattgat ttaagtatta
     1261 tttcatatac agagcttgta acagtaagtt tgcatgctat tcgtcgtttt gaaaagaaat
     1321 ctatttcaaa taaaaataca tttggtattt ggggtgatgg taacttaggt tacattacag
     1381 ccattttatt acgtaaatta tatccagagt ctaaaatata tgtctttggt aaaacagatt
     1441 ataaattgag tcacttctca tttgttgatg atgtcttctt tattaataaa atacctgaag
     1501 gcttaacatt tgatcatgca tttgagtgtg tgggtggtcg cggtagtcaa tcagccataa
     1561 atcaaatgat cgattacatt tcaccagaag gaagcattgc actgttaggt gtaagtgagt
     1621 tcccagtaga agttaataca cgtctagtat tggaaaaagg actaacgttg attggtagta
     1681 gtcgaagtgg ttcaaaagat ttccaagatg ttgtagactt atacattcaa tacccagata
     1741 ttgtagataa attagcgttg ttaaaaggtc aagaatttga aattgcaaca attaatgatc
     1801 ttacagaagc ttttgaagca gacctgtcta catcttgggg taaaacagta ttaaaatgga
     1861 ttatgtaaat agaaaaggat gaattaacgt tggttaaaag taagatatat atagataaaa
     1921 tctattggga acgtgttcag ttattcgttg aaggacatag tgaaaaccta gatttagaag
     1981 atagtaattt tgtattaaga aatttaactg agacacgtac aatgaaggcg aatgatgtca
     2041 aaatagatgg gaatcaattc gtttgtcgtt tcaatgtagc tatcttagat aatggttatt
     2101 acttacctga agataagtac ttattagtga atgagcaaga acttgattat attgcacagt
     2161 taaacccaga tgtgattaat gatgcatatc aaaatctaaa gccagaacaa gaagaagaat
     2221 acaacgaatt agaaacacaa aatggtaaaa tcaatttctt attgcagact tacctaaaag
     2281 aatttagaaa aggcggcatt tcgaagaaaa cggtttatac tgttacacct gaaatttcta
     2341 gcgatgttaa tgaatttgtc cttgatgttg ttgtaacgac tccggaagtt aaaagtattt
     2401 atatcgttcg taaatataaa gaattacgta agtatttccg caaacaatca tttaatacaa
     2461 gacaatttat ttttaaagcg atatttaata cgacgaaatt tttccacttg aaaaaaggga
     2521 atacggtgtt gttcacatca gactctagac caacgatgtc tggaaacttt gaatacatct
     2581 ataacgaaat gttacgtcaa aatttagata aaaagtatga tattcacact gtttttaaag
     2641 cgaatattac agatagacgt ggcatcatcg acaagtttag attgccatat ttacttggga
     2701 aggcagacta catctttgtt gatgactttc acccattgat ttatacagtg cgttttagac
     2761 gttctcaaga agttattcaa gtatggcatg ccgttggtgc ctttaaaaca gttggcttta
     2821 gtcgtactgg taaaaagggt ggaccattta ttgattcatt aaatcatcgt agctatacaa
     2881 aagcttatgt atcatctgaa accgatattc cattctacgc tgaagcattt ggtattaaag
     2941 agaaaaatgt agtgcctaca ggtgttccac gtactgatgt actatttgat gaagcttatg
     3001 cgacacagat caaacaagag atggaagatg aattaccaat tattaaaggt aagaaagtca
     3061 ttcttttcgc accaacattt agaggtagtg gtcatggtac agcacattac ccatttttca
     3121 aaattgattt tgaacgttta gcaagatatt gcgaaaaaaa taacgcggtt gtattattta
     3181 aaatgcatcc atttgtgaaa aatagactta atattgcaga caaacataaa caatattttg
     3241 ttgacgtttc tgactttaga gaagttaatg atatactgtt cataacagat ttat
//
LOCUS       DAKTIO010000221         3286 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.227, whole genome shotgun sequence.
ACCESSION   DAKTIO010000221 DAKTIO010000000
VERSION     DAKTIO010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.227"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            73..1383
                     /gene="sbi"
                     /locus_tag="O0293_002554"
     CDS             73..1383
                     /gene="sbi"
                     /locus_tag="O0293_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-binding protein Sbi"
                     /protein_id="HCY0281410.1"
                     /translation="MKNKYISKLLVGAATITLATMISNGEAKASENTQQTSTKHQTTQ
                     NNYVTDQQKAFYQVLHLKGITEEQRNQYIKTLREHPERAQEVFSESLKDSKNPDRRVA
                     QQNAFYNVLKNDNLTEQEKNNYIAQIKENPDRSQQVWVESVQSSKAKERQNIENADKA
                     IKDFQDNKAPHDKSAAYEANSKLPKDLRDKNNRFVEKVSIEKAIVRHDERVKSANDAI
                     SKLNEKDSIENRRLAQREVNKAPMDVKEHLQKQLDALVAQKDAEKKVAPKVEAPQIQS
                     PQIEKPKVESPKVEVPQIQSPKVEVPQSKLLGYYQSLKDSFNYGYKYLTDTYKSYKEK
                     YDTAKYYYNTYYKYKGAIDQTVLTVLGSGSKSYIQPLKVDDKNGYLAKSYAQVRNYVT
                     ESINTGKVLYTFYQNPTLVKTAIKAQETASSIKNTLSNLLSFWK"
     gene            1919..2848
                     /gene="hlgA"
                     /locus_tag="O0293_002555"
     CDS             1919..2848
                     /gene="hlgA"
                     /locus_tag="O0293_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB subunit A"
                     /protein_id="HCY0281411.1"
                     /translation="MIKNKILTATLAVGLIAPLANPFIEISKAENKIEDIGQGAEIIK
                     RTQDITSKRLAITQNIQFDFVKDKKYNKDALVVKMQGFISSRTTYSDLKKYPYIKRMI
                     WPFQYNISLKTKDSNVDLINYLPKNKIDSADVSQKLGYNIGGNFQSAPSIGGSGSFNY
                     SKTISYNQKNYVTEVESQNSKGVKWGVKANSFVTPNGQVSAYDQYLFAQDPTGPAARD
                     YFVPDNQLPPLIQSGFNPSFITTLSHERGKGDKSEFEITYGRNMDATYAYVTRHRLAV
                     DRKHDAFKNRNVTVKYEVNWKTHEVKIKSITPK"
BASE COUNT         1363 a          560 c          446 g          917 t
ORIGIN      
        1 catttgtatt ctcatgtgat aaattctaaa agcatacaat aaatttaata tgtaaaaaga
       61 aagggaatac acatgaaaaa taaatatatc tcgaagttgc tagttggggc agcaacaatt
      121 acgttagcta caatgatttc aaatggggaa gcaaaagcga gtgaaaacac gcaacaaact
      181 tcaactaagc accaaacaac tcaaaacaac tacgtaacag atcaacaaaa agctttttat
      241 caagtattac atctaaaagg tatcacagaa gaacaacgta accaatacat caaaacatta
      301 cgcgaacacc cagaacgtgc acaagaagta ttctctgaat cacttaaaga cagcaagaac
      361 ccagaccgac gtgttgcaca acaaaacgct ttttacaatg ttcttaaaaa tgataactta
      421 actgaacaag aaaaaaataa ttacattgca caaattaaag aaaaccctga tagaagccaa
      481 caagtttggg tagaatcagt acaatcttct aaagctaaag aacgtcaaaa tattgaaaat
      541 gcggataaag caattaaaga tttccaagat aacaaagcac cacacgataa atcagcagca
      601 tatgaagcta actcaaaatt acctaaagat ttacgtgata aaaacaaccg ctttgtagaa
      661 aaagtttcaa ttgaaaaagc aatcgttcgt catgatgagc gtgtgaaatc agcaaatgat
      721 gcaatctcaa aattaaatga aaaagattca attgaaaaca gacgtttagc acaacgtgaa
      781 gttaacaaag cacctatgga tgtaaaagag catttacaga aacaattaga cgcattagtt
      841 gctcaaaaag atgctgaaaa gaaagtggcg ccaaaagttg aggctcctca aattcaatca
      901 ccacaaattg aaaaacctaa agtagaatca ccaaaagttg aagtccctca aattcaatca
      961 ccaaaagttg aggttcctca atctaaatta ttaggttact accaatcatt aaaagattca
     1021 tttaactatg gttacaagta tttaacagat acttataaaa gctataaaga aaaatatgat
     1081 acagcaaagt actactataa tacgtactat aaatacaaag gtgcgattga tcaaacagta
     1141 ttaacagtac taggtagtgg ttctaaatct tacatccaac cattgaaagt tgatgataaa
     1201 aacggctact tagctaaatc atatgcacaa gtaagaaact atgtaactga gtcaatcaat
     1261 actggtaaag tattatatac tttctaccaa aacccaacat tagtaaaaac agctattaaa
     1321 gctcaagaaa ctgcatcatc aatcaaaaat acattaagta atttattatc attctggaaa
     1381 taatcaatca aaaatatctt ctctagtttt acatcatttt ttaaataatt ttcgtaacaa
     1441 accgtgatta aaaagaaccg ttgattctca atcgaatcta cggttctttt ttcattttcc
     1501 atcaattaaa tgcttcttcg ctatttgtca gcccactttt ttacctgcaa cttgttaaat
     1561 aatccttaca tcgttaacga atagttcatc atttagttga atcagctcaa ctttattaac
     1621 ttcatatttt cacaaactat tgcgcaatcc attccttttc cactacaagc accataatta
     1681 aacaacaatt caataaaata agacttgcaa agcatagtta tgtagctata taaacgcctg
     1741 cgaccaataa atcttttaaa cataacataa tgcaaaaaca tcatttaaca atgctaaaaa
     1801 tgtctcttca atacatgttg atagtaatta acttttaacg aacagttaat tcgaaaacgc
     1861 ttacaaatgg attattatat atatgaactt aaaattaaat agaaagaaag tgatttctat
     1921 gattaaaaat aaaatattaa cagcaacttt agcagttggt ttaatagccc ctttagccaa
     1981 tccatttata gaaatttcta aagcagaaaa taagatagaa gatatcggcc aaggtgcaga
     2041 aatcatcaaa agaacacaag acattactag caaacgatta gctataactc aaaacattca
     2101 atttgatttt gtaaaagata aaaaatataa caaagatgcc ctagttgtta agatgcaagg
     2161 cttcattagc tctagaacaa catattcaga cttaaaaaaa tatccatata ttaaaagaat
     2221 gatatggcca tttcaatata atatcagttt gaaaacgaaa gactctaatg ttgatttaat
     2281 taattatctt cctaaaaata aaattgattc agcagatgtt agtcagaaat taggctataa
     2341 tatcggcgga aacttccaat cagcgccatc aatcggaggc agtggctcat tcaactactc
     2401 taaaacaatt agttataatc aaaaaaacta tgttactgaa gtagaaagtc agaactctaa
     2461 aggtgttaaa tggggagtga aagcaaattc attcgttaca ccgaatggtc aagtatctgc
     2521 atatgatcaa tacttatttg cacaagaccc aactggtcca gcagcacgag actatttcgt
     2581 cccagataat caactacctc ctttaattca aagtggcttt aatccatcat ttattacaac
     2641 attgtcacac gaaagaggta aaggtgataa aagcgagttt gaaatcactt acggcagaaa
     2701 catggatgct acatatgctt acgtgacaag acatcgttta gccgttgata gaaaacatga
     2761 tgcttttaaa aaccgaaacg ttacagttaa atatgaagtg aactggaaaa cacatgaagt
     2821 aaaaattaaa agcatcacac ctaagtaaac agttcaatca tcttaaaaaa tcctgggaca
     2881 cttcatactt gtctcaggat tttttaacaa attgaatcag ccttataaca ttaaattatt
     2941 ttatcgtaca ttaaatttaa taataacaac tgatttttat aagaataaag tatcgaacca
     3001 tagtagatac acaaataata caaatgaaac aatttaactt gaaagcttaa ataaatatta
     3061 tcaagttaat aaacaattaa tttttagatg gattcatcaa aaatcgtaaa aagcacaatt
     3121 tgtattttac aaacattaat taaaaaagaa agcaagacat tcgtgcaatc ggttacctta
     3181 aattgtttac aactgtcaac aataccaagg ttttattaac tatatttctc acaaaattag
     3241 cttttagcat tccaaacaaa aaaggttaaa tcgaacggaa ttatgg
//
LOCUS       DAKTIO010000222         3280 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.228, whole genome shotgun sequence.
ACCESSION   DAKTIO010000222 DAKTIO010000000
VERSION     DAKTIO010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.228"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(162..632)
                     /locus_tag="O0293_002556"
     CDS             complement(162..632)
                     /locus_tag="O0293_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281412.1"
                     /translation="MKRKVLVLTMGVICATQLWHSNHANALVTESGANDTKQFTEIVS
                     EEKVITVEHAQINIFQSNSNSNLMEFNILTMGGKSGAMVGYSEIDSSHFTDRDKRVIR
                     RDHVKEAQSLVENYKDTQSADARMKAKQKVNTLSKPHQNYFNKQIDKVYNGLQR"
     gene            complement(967..1989)
                     /gene="emp"
                     /locus_tag="O0293_002557"
     CDS             complement(967..1989)
                     /gene="emp"
                     /locus_tag="O0293_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix protein-binding adhesin
                     Emp"
                     /protein_id="HCY0281413.1"
                     /translation="MKKKLLVLTMSTLFATQIMNSNHAKASVTESVDKKFVVPESGIN
                     KIIPAYDEFKNSPKVNVSNLTDNKNFVASEDKLNKIADSSAASKIVDKNFVVPESKLG
                     NIVPEYKEINNRVNVATNNPASQQVDKHFVAKGPEVNRFITQNKVNHHFITTQTHYKK
                     VITSYKSTHVHKHVNHAKDSINKHFIVKPSESPRYTHPSQSLIIKHHFAVPGYHAHKF
                     VTPGHASIKINHFCVVPQINSFKVIPPYGHNSHRMHVPSFQNNTTATHQNAKVNKAYD
                     YKYFYSYKVVKGVKKYFSFSQSNGYKIGKPSLNIKNVNYQYAVPSYSPTHYVPEFKGS
                     LPAPRV"
     gene            complement(2340..>3280)
                     /locus_tag="O0293_002558"
     CDS             complement(2340..>3280)
                     /locus_tag="O0293_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="coagulase"
                     /protein_id="HCY0281414.1"
                     /translation="LDLIMGYKDEERANKKAVNKRMLENKKEDLETIIDEFFSDIDKT
                     RPNNIPVLEDEKQEEKNHKNMAQLKSDTEAAKSDESKRSKRSKRSLNTQNHKPASQEV
                     SEQQKAEYDKRAEERKARFLDNQKIKKTPVVSLEYDFEHKQRIDNENDKKLVVSAPTK
                     KPTSPTTYTETTTQVPMPTVERQTQQQIIYNAPKQLAGLNGESHDFTTTHQSPTTSNH
                     THNNVVEFEETSALPGRKSGSLVGISQIDSSHLTEREKRVIKREHVREAQKLVDNYKD
                     THSYKDRINAQQKVNTLSEGHQKRFNKQINKVYNGK"
BASE COUNT          939 a          497 c          494 g         1350 t
ORIGIN      
        1 taaaaagcct acaacatgaa tcgttgcaga ctatcatatc gagacaaaga ggtttattag
       61 tgtctctata tctatgtgga atttgaaaag attatttata atatttacgg ggataaattg
      121 tcatttaaaa acgagatgta taacttataa tttactttgg attagcgttg taatccatta
      181 taaaccttat caatttgttt attgaaatag ttttgatgcg gtttgcttaa tgtgttaact
      241 ttttgtttgg ctttcatcct agcatcagca ctttgtgtat ctttatagtt ctctactaag
      301 ctttgtgctt ctttaacatg atcacgtcta ataacgcgtt tgtcacggtc tgtgaaatgt
      361 gatgagtcaa tttcactata accaaccata gctcctgatt taccgcccat tgttaatatg
      421 ttgaactcca tcaagtttga attgctatta gattgaaaaa tattaatttg agcatgttca
      481 actgttataa ctttctcttc cgatacaatt tcagtaaatt gcttagtatc atttgcgcca
      541 ctctctgtta ctaatgcgtt tgcgtgatta gaatgccata attgagttgc acaaattaca
      601 cccattgtta gtactaatac tttccttttc atttgtttat gctcccttat ttgctttgat
      661 ataaaaaccg actttttcac cctgttttct tcgccattga aaattatata ccaatatttt
      721 agaaacaata attaacttgc gatgaacaaa ctattaacaa taatcttgag tattatattt
      781 atcttaatta ataatatatt tatttaagat tatgttactt ccaactttca aagtagaaaa
      841 acggtataat ttgttgatgg gtgtttattg ataaactgca aaaaatacag cctacaacca
      901 catagattgt agactatatt taaaataata ggtatttatc atatctcgta aacttagtgc
      961 caatttttat actcgtggtg ctggtaagct acccttaaat tcaggaacgt agtgtgtagg
     1021 gctataactt ggaacagcat attgataatt tacatttttg atatttaatg atggtttccc
     1081 aattttataa ccatttgatt gtgaaaatga gaaatatttc ttcacacctt taactacttt
     1141 ataagaatag aagtatttat agtcatatgc tttatttact ttagcatttt gatgtgttgc
     1201 tgttgtgtta ttttggaaac ttggtacatg catacgatgt gaattgtgac catatggtgg
     1261 aattaccttg aaactattta tttgtggcac aacacaaaag tgattaattt taatgctagc
     1321 atgccctggt gtaacaaatt tatgcgcgtg atatccagga actgcaaaat gatgcttgat
     1381 aattaaagat tgagatggat gtgtatatct aggcgattct gatggtttaa caataaagtg
     1441 tttattaata gaatcctttg catgatttac atgtttatgt acatgtgttg atttgtatga
     1501 agtaataact ttcttgtagt gggtttgcgt agtaatgaag tggtggttta ctttgttttg
     1561 cgtaataaat ctatttactt ctgggccttt agcaacaaaa tgcttatcaa cttgttgtga
     1621 agctggattg tttgttgcta cattcacgcg attattgatt tctttgtact ctggcacaat
     1681 gtttcctaac tttgattctg gtacgacaaa gtttttatct acaattttac tagctgccga
     1741 tgaatctgca atcttattca atttatcttc agaagctaca aagtttttat tgtcagttaa
     1801 attactaaca tttacttttg gcgaattctt aaattcatcg taagctggaa taattttatt
     1861 aattcctgat tctggaacta caaatttttt gtcaacactc tctgtcactg atgctttagc
     1921 gtgatttgaa ttcataattt gtgtagcaaa tagcgtgctc atagttaaaa ctaataattt
     1981 ctttttcatc tgttatttct cctttatata gactcaatat tataaccaat ataatttccc
     2041 tgttatattc actaacagca ttatatacca gaattttcag tataataatt aacttgaagt
     2101 aaacgttgtc ttaacatttt tattgttttt cagcttaaaa ttaattattg atattgatag
     2161 ttaagcataa taattttttc gtaatataaa gtgaaaaaag taatagtcca cacctgttta
     2221 gaatgtggac tatactagat tgcatcattg aaatgatgac tttgatatta tttattgcta
     2281 gtttaaaatg ttgttatttt tacgacaaac tcattatttg ctttgcagcc atgcattaat
     2341 tatttgccat tatatacttt attgatttgt ttattaaaac gtttttgatg accttcactt
     2401 aaagtattta ctttttgttg tgcatttatt cggtctttat aactatgtgt atctttataa
     2461 ttatcaacta acttttgagc ttctctaacg tgttcacgct taattacacg cttctcacgt
     2521 tcagttagat gagaagaatc aatttgactt ataccaacca gtgatcctga ttttctacca
     2581 ggtaaagcag acgtttcttc aaattcaaca acattattat gcgtgtgatt tgaagttgtt
     2641 ggtgattgat gcgttgttgt gaaatcatga ctttcaccat ttaatccagc caattgtttt
     2701 ggtgcattat aaataatttg ttgctgagtt tgacgctcaa ctgtaggcat tggtacctgt
     2761 gtcgttgttt cagtatatgt agtcggtgat gttggtttct ttgttggtgc agaaaccaca
     2821 agtttcttgt cgttttcgtt gtcaatacgt tgtttatgct caaaatcata ttctaatgac
     2881 actacaggtg ttttcttaat tttttgatta tccaaaaatc tcgcttttct ttcttctgct
     2941 cttttatcat attcagcttt ttgttgttca gaaacttctt gagatgcagg tttgtgattt
     3001 tgagtattta aacttctttt acttctcttg cttctttttg attcatcact ttttgctgct
     3061 tcagtgtcag attttaattg agccatattt ttatgatttt tctcttcttg tttttcatct
     3121 tctaaaacag gaatattatt aggtcttgtt ttatctatat cactaaaaaa ttcatcaatt
     3181 atggtttcta agtcttcttt tttattttct aacatccttt tgttaactgc ttttttattt
     3241 gctctttctt catctttata tcccataatt aaatctaact
//
LOCUS       DAKTIO010000223         3251 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.229, whole genome shotgun sequence.
ACCESSION   DAKTIO010000223 DAKTIO010000000
VERSION     DAKTIO010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.229"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..446
                     /locus_tag="O0293_002559"
     CDS             <1..446
                     /locus_tag="O0293_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0281415.1"
                     /translation="YLSESEEIMMSNNSINFISSGNIRKAIEKNNYDKVISFIKINDS
                     NYRLSNVDTMKVTLYSNGSNYDKEALLINKDEFCPLRKITLDNKLDSQRVMEIDSLAA
                     IINLVKQGKGKALLPMTFENKRDIVQDISKIFEVNYYTYNHIMHH"
     gene            880..1482
                     /locus_tag="O0293_002560"
     CDS             880..1482
                     /locus_tag="O0293_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281416.1"
                     /translation="MLISLVIPVFALLVIGGIIWMIIEGIVHISKKNKAIDNFFNQVN
                     KVSETYKFATTFLFLILATAGISQFYLYYIVSAFLFWLVILTFGIAGIIFLMPYGLCF
                     LPFYKQKKKKQTFKKYMVYTTIGLSICLGLSLVLVHTTKIYMDEGGVRYYYGSFVMKQ
                     AGGYAYLALAVLSTLLIVAKKATNKNKEIETVDNTNITER"
     gene            1749..>3251
                     /locus_tag="O0293_002561"
     CDS             1749..>3251
                     /locus_tag="O0293_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA2/NAM7 family helicase"
                     /protein_id="HCY0281417.1"
                     /translation="MSQLLNDTLSAWLLIESLSPGEVNFTAEDILSAEHFKNGAKQAQ
                     LQSFDEYFEIWNAERFIISEEKSETGELIFKFYRHCFRYNEINLKIQDIFDDYSEIHN
                     PNGTHCYGYTFNTDKHGKVIVDSIHIPMIMSALKEIEKNKNANIEEKFNDSVEKFVQK
                     VKEILADEPINEFKLKKMDKAYDEYFSVLNSKKDGLFGHYVAIEYVKDSDLPQPEFNS
                     FFISDIEKARKSPNQTLIDYIEGVEESQHIEVDENKEMFDKFLHPSRLPDGRWPSQTE
                     FRLSLMQQLAVNQITSGNERISSVNGPPGTGKTTLLKDIFAHLVVERGKELAKLNNPK
                     DAFVKTKIHETDDKYVYLLKESIAKYKMVVASSNNGAVENISKDLPKIEEIIRNPEKC
                     KFPKYEQNYANLAHELKDFAEIAEDLIGESAWGLFSGVFGKSTNINQVLSHMLKQDAN
                     DIGFAKLLQNENNRMSYNELMSEWQSHQRAFLEELRHVEMLKEESIRAYDV"
BASE COUNT         1235 a          376 c          564 g         1076 t
ORIGIN      
        1 attatttatc tgagagcgaa gaaattatga tgtcgaataa ctcaattaat tttatttcta
       61 gtggaaatat tagaaaagct atagaaaaaa ataattatga taaggttatt tcattcataa
      121 aaattaacga ctcaaattat agacttagta atgttgatac tatgaaagta acgctttaca
      181 gtaatggaag taattatgat aaagaggctt tactaataaa taaagatgag ttttgtcctt
      241 taaggaaaat aactttagat aataagcttg attcacaacg ggtaatggaa attgattcat
      301 tggcagcaat aataaactta gttaaacaag gaaaaggaaa ggctttatta cctatgactt
      361 ttgagaataa aagagatata gtacaggaca tttctaagat atttgaagtt aactactata
      421 cttataatca tattatgcat cattaattga aagttgattg agtttaaatt acaggaacga
      481 ttttaaaagt ctagtagtta gaatgagcga tgtgacagaa aagaaattat gccttttatt
      541 ttgtaaattc gttgttttaa agtaaaggtc cttgtagtat aggaaaaagt ggatgacttc
      601 tccgccttta ccaccctttc aagcataatg ataactgcta aatattgatt atagcaccat
      661 tgcaaaacta atcattaatt gtcatattca actattttga aaagtatcaa tttcacatta
      721 tcaaattatt gagtatctca atttattgtt atacacaaac agtatttttg ttatcataga
      781 gttaataaat ataataaatt aaacaagtaa ctttacaaaa attagaatga ttcaattcaa
      841 tcataattgt ttgaaaatag aggtgagcag gtgaacgata tgttaattag tcttgtaatc
      901 ccagtttttg ctttgttagt tattggtggt attatttgga tgattataga aggtatagta
      961 catatttcaa aaaagaataa agcaattgat aactttttta accaagttaa taaagtaagt
     1021 gagacatata aattcgctac tactttttta tttctaatct tggctacggc tggtatttct
     1081 caattttatc tatattatat agtatcagcg tttctttttt ggcttgtcat acttaccttt
     1141 ggcattgcag gtatcatttt tttaatgcca tatggattat gttttctacc gttttataag
     1201 caaaaaaaga aaaaacagac atttaaaaaa tacatggttt acactacgat tggtttgtca
     1261 atttgtctag gcttatctct agttttggtt cacactacga aaatttatat ggacgaaggt
     1321 ggcgtaagat actattacgg tagttttgta atgaaacaag cgggcggtta tgcttattta
     1381 gctttagcgg tactttcaac gttgttaatt gttgcgaaaa aagctacaaa taaaaataaa
     1441 gaaatcgaaa ccgtcgacaa tacaaatata acggaaagat aattaaggga gtgctcattc
     1501 aggagtgctc ttttttgatg tccaaattta gttgcaaatg aaggcataga agaaattttt
     1561 atagtcattt attggataat gaattatggt catatcgatt aattttatta gtgaggattt
     1621 tacaaacatt tgttgaataa atatggtaat gataatatat gaatattttt gaaaaaacac
     1681 taatatgatt aaattatttg ttaaaatagc attaagtaaa aaacaaatag ggaacaaggt
     1741 gggatttcat gagtcaattg ctaaatgata cgttatcggc ttggttgtta attgaatctt
     1801 taagtccagg agaagtaaat tttacagcgg aagatatact ctcagctgaa cattttaaaa
     1861 atggtgcaaa gcaagcacaa cttcaaagtt ttgatgaata ttttgaaata tggaatgctg
     1921 aacgctttat tatatcagaa gaaaaatcag agactgggga acttatattt aaattttata
     1981 gacattgctt ccgctataat gaaattaatt tgaaaattca ggatattttt gatgattatt
     2041 ctgagattca taatccaaat gggacacact gttatggtta cacatttaac acagataaac
     2101 acggcaaagt gatagttgat tctatacata ttccgatgat tatgagtgca ttaaaagaaa
     2161 ttgaaaagaa caaaaatgcc aatatagaag aaaaatttaa tgattctgtt gaaaaatttg
     2221 ttcaaaaagt aaaagaaatt ttagcagatg aaccaattaa tgaatttaaa ttgaagaaga
     2281 tggacaaagc ttatgatgag tacttttctg tattaaattc aaagaaagat ggattatttg
     2341 gacattatgt agcaatagaa tatgtgaaag atagtgattt accacagccg gaatttaaca
     2401 gcttcttcat aagtgatatt gagaaagcaa gaaaatctcc caaccaaact ttaattgatt
     2461 acattgaagg tgtagaagaa agtcagcaca tagaagtaga tgaaaataaa gaaatgtttg
     2521 acaaattttt acatccttca cgtttgcctg atggacgatg gccatcacag actgagttta
     2581 gattgtcttt aatgcaacaa cttgctgtaa accaaattac gagtggtaat gaaagaataa
     2641 gttcagttaa tgggccacca ggtacaggta agactacttt attaaaagat atatttgctc
     2701 atctagtagt tgaaagaggt aaagagttag ctaaactaaa taatcctaaa gatgcatttg
     2761 tcaaaacaaa aattcatgaa acggatgata aatacgtata cttactaaag gaatctattg
     2821 ccaaatataa gatggtagtc gcatctagta ataatggagc tgttgaaaat atatctaaag
     2881 atttaccgaa aattgaagaa attataagaa atcccgaaaa atgtaaattc cctaaatatg
     2941 aacagaatta tgcaaattta gcacatgaat taaaagattt tgctgaaata gctgaagatt
     3001 tgattggtga aagtgcctgg ggcttatttt ctggagtttt tggtaaaagt actaatatta
     3061 accaagtatt gagtcatatg ttaaaacaag atgcgaatga tattggcttt gctaaattac
     3121 tacaaaatga gaataatcgt atgagttata acgagttaat gagtgaatgg caatcacatc
     3181 aacgtgcatt tttagaagag ttgaggcatg ttgaaatgtt aaaagaagaa tctattagag
     3241 catatgatgt t
//
LOCUS       DAKTIO010000224         3203 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.230, whole genome shotgun sequence.
ACCESSION   DAKTIO010000224 DAKTIO010000000
VERSION     DAKTIO010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.230"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            140..775
                     /locus_tag="O0293_002562"
                     /pseudo
     CDS             140..775
                     /locus_tag="O0293_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            772..1302
                     /gene="sat4"
                     /locus_tag="O0293_002563"
     CDS             772..1302
                     /gene="sat4"
                     /locus_tag="O0293_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HCY0281418.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFEKAVFWYLRF"
     gene            1395..2189
                     /locus_tag="O0293_002564"
                     /note="aph(3')-IIIa"
     CDS             1395..2189
                     /locus_tag="O0293_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCY0281419.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            2465..3036
                     /locus_tag="O0293_002565"
                     /pseudo
     CDS             2465..3036
                     /locus_tag="O0293_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
BASE COUNT         1075 a          504 c          729 g          895 t
ORIGIN      
        1 cgaacactga catgatatat tagtgattag ctatattttt tactttgcaa cagaaccatc
       61 atttttatta aaatactctt ttgtaaaatc tttctgctct tcaatgtatt taatttcttt
      121 acctctaaat ctattatagc tatttgatga ttacaataaa attgatctta ccttattgcc
      181 cttggaagag ttagataatt acctaaaggg cgataaatta ataaaggttc taattgataa
      241 agattgtaga attaaaaggg acatagttcc gactgatata gattatcatg taagaaagcc
      301 aagcgcaagg gagtatgatg attgctgcaa tgaattttgg aatgtaacac cttatgttat
      361 taaaggattg tgccgtaagg aaattttatt tgctattgat cattttaatc agattgttcg
      421 ccatgagctg ctgagaatga tatcatggaa ggtcggcatc gaaacaggct ttaaatgaag
      481 tgtaggcaag aactataagt ttattgaaag gtatatatcc gaggatttgt gggagaaact
      541 tttgtccacc taccggatgg attcctatga aaacatatgg gaagcattat ttctatgcca
      601 tcaattgttc agggcggtat ccggtgaggt ggcggaaagg cttcattatg cctatccgga
      661 gtatgatagg aatataacaa aatataccag ggacatgtat aaaaaataca ctggtaaaac
      721 cggctgcctg gatagcacat atgccgctga tatagaagag aggcgggaac agtgattaca
      781 gaaatgaaag cagggcacct gaaagatatc gataaaccca gcgaaccatt tgaggtgata
      841 ggtaagatta taccgaggta tgaaaacgag aattggacct ttacagaatt actctatgaa
      901 gcgccatatt taaaaagcta ccaagacgaa gaggatgaag aggatgagga ggcagattgc
      961 cttgaatata ttgacaatac tgataagata atatatcttt actaccaaga cgataaatgc
     1021 gtcggaaaag ttaaactgcg aaaaaattgg aaccggtacg cttatataga agatatcgcc
     1081 gtatgtaagg atttcagggg gcaaggcata ggcagcgcgc ttatcaatat atctatagaa
     1141 tgggcaaagc ataaaaactt gcatggacta atgcttgaaa cccaggacaa taaccttata
     1201 gcttgtaaat tctatcataa ttgtggtttc aaaatcggct ccgtcgatac tatgttatac
     1261 gccaactttg aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa
     1321 ttggagttcg tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag
     1381 gaaggaaata ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa
     1441 aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg
     1501 gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg
     1561 atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg
     1621 tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg
     1681 tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg
     1741 cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct
     1801 tagacagccg cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt
     1861 gcgaaaactg ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa
     1921 agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca
     1981 tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg
     2041 acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac
     2101 agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat
     2161 attatatttt actggatgaa ttgttttagt acctagattt agatgtctaa aaagctttaa
     2221 ctacaagctt tttagacatc taatcttttc tgaagtacat ccgcaactgt ccatactctg
     2281 atgttttata tcttttctaa aagttcgcta gatagagttc tatattagag atgtaaagag
     2341 ttttggtgaa gaggtttgtg agtatggctt ttataaccag ggtgcaatga gaagcactaa
     2401 cagcagctag cttaagaacg cataggatat actttttatg gaagtcacag aggaggaata
     2461 aaatttgaac aggattaaca gagtaacctc tattcttatt cagctgcaat caaaaaaata
     2521 attcctgcca aagaaattgc acagcgattt aatataagct taaggacagt ttacagagat
     2581 atccggacac ttgaagaagc cggaatacca attggatctg aggccggaaa aggatatttt
     2641 cttgtagagg gcttcctact tctgccggta atgtttacag cggcggaagt gggtgcattg
     2701 attacagcag ggaaattttt aaattgccat ggagatgaat catttataaa ggattttgat
     2761 tcagctatgt ataaaatcaa atctattttg aagcatggtg aaaaaaacta tgcacaggag
     2821 ttggagaaca gtattaacgt gtacagcaca tctggacaga aaaatacatt ggcggataac
     2881 gttattgcag caattcagac tgcaatctgc aataaaaggg taatatcaat tcaatatcct
     2941 gcatcaggag ggcaagaacc ggaaagcaga atgatagagc ctatatcact ggggttttat
     3001 gaacagaact ggtacttaat cggttttgca ggctaagaaa tgaatatagg aattttcgtg
     3061 tggatcggat taaaagcatt tgcatagaag aagagctctg tcaaagccat gatggctctt
     3121 tggaacaaat attaaaacaa atgctggttc tgttgcaaag taaaaaaata tagctaacca
     3181 ctaatttatc atgtcagtgt tcg
//
LOCUS       DAKTIO010000225         3138 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.232, whole genome shotgun sequence.
ACCESSION   DAKTIO010000225 DAKTIO010000000
VERSION     DAKTIO010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.232"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..277
                     /locus_tag="O0293_002566"
     CDS             <1..277
                     /locus_tag="O0293_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HCY0281420.1"
                     /translation="MRRFISYINSPIAITSANISGTADDILITENEAIKHMGENVRYM
                     LRSQNKTNYKTSSTIIKVTDNKIELLREGDIKFEEIKERLGTGIIYE"
     gene            270..1388
                     /locus_tag="O0293_002567"
     CDS             270..1388
                     /locus_tag="O0293_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281421.1"
                     /translation="MNKLILGIYLYRIFSRAYFYLPFLLIYFLIQGYSIIQLEILMAS
                     YGIAAFLFSLYKEKCFKICNLKDSNKLVVSEIFKIIGLLLLLYQNQYLILVVAQILLG
                     LSYSMMAGVDTAIIKRNITNEKYVQNKSNSYMFLSLLISGIIGSYLYGINIKWPIIMT
                     GIFSILTIIIIRCTLVENRELNLIGETKGKIKKFLPEEKFWILHYSFLRALILGFFIG
                     FIPINIYNDLKLNNLQFISVLTCYTVMGFVSSRYLTKYLNYKFVSEICLVIFLIIYTY
                     QSFIAVTISMIFLGISSGLTRPQTINKLSSSSNLRVMLNYAETLYFIFNIAFLLMGGY
                     LYTIGTIQYLILFISLLIFIYLIIIFYFTRREQHENKN"
     gene            1372..1887
                     /locus_tag="O0293_002568"
     CDS             1372..1887
                     /locus_tag="O0293_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HCY0281422.1"
                     /translation="MKIKTEFKGNNIPYEYAAGADVSDSINGNPIKSFPFEVIELPEG
                     TKYLAWSLIDYDAIPVCGFAWIHWSVANVSVSGNSISIKADLSRTKGDYVQGKNSFTS
                     GLLAEDFSEIENHYVGPTPPDQDHQYELTVYALDHSLNLKNGFYLNEFLKEVNQHKID
                     QTSINLIGRKI"
     gene            2198..2632
                     /locus_tag="O0293_002569"
     CDS             2198..2632
                     /locus_tag="O0293_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCY0281423.1"
                     /translation="MKLKSFITVTLALGMIATTGATVAGNEVSAAEKDKLPATQKAKE
                     MQNVPYTIAVDGIMAFNQSYLNLPKDSQLSYLDLGNKVKALLYDERGVTPEKIRNAKS
                     AVYTITWKDGSKKEVDLKKDSYTANLFDSNSIKQIDINVKTK"
     gene            complement(2889..3074)
                     /locus_tag="O0293_002570"
     CDS             complement(2889..3074)
                     /locus_tag="O0293_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HCY0281424.1"
                     /translation="MKHLVTFFWALLLMQMVNFVLNSLVGGDSLNVVNPIIMAVLFTI
                     FTAIFAAVIKPPKDSSQ"
BASE COUNT         1194 a          368 c          481 g         1095 t
ORIGIN      
        1 gatgagaaga tttatttcat atattaattc acctatagca attacttcag caaatatatc
       61 tggaactgcc gatgatattt taataactga aaatgaagca attaagcaca tgggcgaaaa
      121 tgtaagatat atgctaagaa gtcaaaataa aactaactat aaaacatcta gtacgattat
      181 taaagtgaca gataataaaa ttgaattatt aagagaagga gatataaagt ttgaagaaat
      241 aaaagaaaga ctaggtacag gtattattta tgaataagtt aatacttggg atttatttat
      301 accgaatttt ttcacgagca tacttttatt taccgttttt attaatttac tttttgattc
      361 aaggttattc cataatacaa ttagaaatat taatggcgtc ttatggcatt gcagcatttt
      421 tattctctct atacaaagag aagtgtttta aaatttgtaa cttaaaagat tctaataaat
      481 tagttgttag tgaaatattc aaaatcatcg gtttattgtt gttattatat caaaatcaat
      541 atttaatttt agtagtggca caaatattat tagggttaag ttactcaatg atggcgggtg
      601 ttgataccgc aataattaaa agaaatataa caaatgagaa atacgtacaa aataagtcaa
      661 atagctatat gttcctatca ttattaattt cagggattat aggtagttat ctttatggaa
      721 taaatattaa atggcctata ataatgactg gtatattttc aattctaaca attataatta
      781 ttcgatgcac attagttgaa aatagggaat taaatttaat aggagaaaca aagggaaaga
      841 taaagaaatt tctaccagaa gagaagtttt ggatattgca ttattctttt ttaagagcgt
      901 taatattagg attttttata ggatttattc caattaatat atataatgat ttaaaactga
      961 ataatttaca atttatttca gtattaactt gttacacagt tatgggtttt gtatcttcac
     1021 gttatttaac taaatacttg aattataagt ttgtgtcaga aatttgttta gtaatatttt
     1081 taataatata tacatatcaa agtttcatag cagttactat ttctatgata tttttaggta
     1141 tttcttcagg gttaactcgt ccacaaacta taaataaact ttctagcagt agtaacttaa
     1201 gagtgatgct taattatgca gaaacgttat attttatttt taatatcgca tttttactta
     1261 tgggtggtta cttatataca ataggaacta ttcaatactt aatattattt atttcgttat
     1321 taatttttat atatttaata ataatatttt attttacaag gagagagcaa catgaaaata
     1381 aaaactgaat ttaaagggaa caatatacca tatgaatacg cagcaggtgc agatgtgagt
     1441 gattctatta acgggaatcc aattaagtca tttccatttg aagtaattga attaccggaa
     1501 gggactaaat atcttgcttg gtctttaatt gactatgatg caattcctgt atgtggcttt
     1561 gcttggattc attggagtgt agctaatgta agtgttagtg gcaattcaat ttctataaaa
     1621 gcagatttat caagaacaaa gggcgactat gtacaaggta aaaatagctt tactagtggg
     1681 ttgttggctg aagatttttc agaaatagaa aatcactatg taggacctac accacctgat
     1741 caagatcatc aatatgaatt aacagtttat gcgttagatc attctttaaa tttgaagaat
     1801 gggttctact tgaatgaatt tttaaaagaa gtaaatcaac ataaaattga tcaaacaagt
     1861 attaacctta taggaagaaa aatttaatac taaatatctc atcaatataa aattgttcaa
     1921 ttaaaagtac aaagaaacaa aggttttaat ttatatatta ggtacggcgt tcgctataat
     1981 gcaaagaagt aattaaattt aagaaatgta aacttagtta ttgtaatgtg aatttatttg
     2041 aaaaaataga aagtattaac aattatagct tttacattaa ttaaaattta tttttaaaaa
     2101 caagtaaaca atttacatac ttataatttt tgaaactttt caatttgtgt tatattgatt
     2161 ttgtaagata ctttaactca caaaggagag agattatatg aaattaaaat catttataac
     2221 tgtaactttg gcactgggca tgatcgcaac gactggcgct actgtggcag gtaatgaggt
     2281 atctgcagca gaaaaggaca aactaccggc aactcaaaaa gctaaagaaa tgcaaaatgt
     2341 tccatataca attgcagtag atggcattat ggctttcaat caatcttact taaatttacc
     2401 aaaagatagc caattatcat atttagattt aggaaataaa gttaaagctt tgttatatga
     2461 tgaacgcggt gtaacacctg agaagattcg aaatgcaaaa tctgccgttt acacgattac
     2521 ttggaaagat ggtagtaaaa aagaagtgga tcttaagaaa gatagctaca cagcaaactt
     2581 gtttgattca aattcaatta aacaaattga tattaatgta aaaactaaat aaacacttaa
     2641 aattatcaaa ttcaacatga agcatttaat tttacagtga tgattataaa ataattgcct
     2701 tgatacaaag attactcgta aatgacatct ttgtattaag gctttttcta aatttaaaag
     2761 tgatgggtta gaggtcattg agctttaaaa tattcaaaat acaaaacatt aatggccaaa
     2821 aataaaagcc gcctttatct gggcagcttc aataataaga aagacatatt tcattttata
     2881 ctaaatagtt attgtgatga atctttcggc ggtttaatta ctgcagcaaa aattgctgtg
     2941 aaaatcgtga acaatactgc catgataatt ggattcacta catttaagct gtctccacct
     3001 actaggctat taagtacaaa gttaaccatt tgcattaata ataatgccca aaagaatgtt
     3061 acgaggtgtt tcatgtcatt ctacctccac tttaattata tatattttat tttaagtgaa
     3121 agttagaaat ttgtatag
//
LOCUS       DAKTIO010000226         3125 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.233, whole genome shotgun sequence.
ACCESSION   DAKTIO010000226 DAKTIO010000000
VERSION     DAKTIO010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.233"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(55..243)
                     /locus_tag="O0293_002571"
     CDS             complement(55..243)
                     /locus_tag="O0293_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281425.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILM
                     GINAIYLVVKVMNFKKNN"
     gene            complement(826..1821)
                     /locus_tag="O0293_002572"
     CDS             complement(826..1821)
                     /locus_tag="O0293_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HCY0281426.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADIL
                     EILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQK
                     VIEKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFM
                     RSTEVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVAN
                     VKAIKPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSY
                     GGLLKQKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
     gene            2273..2854
                     /locus_tag="O0293_002573"
     CDS             2273..2854
                     /locus_tag="O0293_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281427.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIA
                     DKNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDI
                     ENKKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVA
                     KKLAKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
BASE COUNT         1139 a          519 c          379 g         1088 t
ORIGIN      
        1 aaaaaaacag tgtgaaaaca agaagaaact cgtttcaaaa tcccctcaaa tattctaatt
       61 attctttttg aaattcataa ccttaacaac caaataaata gcatttattc ccatcaaaat
      121 aacatacgga attaatatat ataaattttc caaatgagat ataaaaatta atccaagtaa
      181 aaagaaaaca tttaaaatag ttgctataac gtaaccttca tctaacactg tgtttttatt
      241 caaaataaac actccttttt taaaaaactt tatcacaaga cttactcaaa ttataaatac
      301 ctttattttg agttttaagg gggtatttta aaggtttagg gggtatcata taggttttta
      361 acttaaaaat atatacaagc tcttaaaacg caaatatgag ccaaataaat atatggtaat
      421 ttcacaaaca aaaatttcag caaaaccagt gactaatttt ttaacactgc ccatttacat
      481 gcaaattaaa aattagcata aaatccttta aaacttccac atgtttttct aaattcctgc
      541 agggcgtttt ttatcaaaaa tcgagaattt ttaatttttc tctaaaaact ccgtaagagc
      601 ataaggagaa tttgctatca ttcaaaaatg ataggtctgg caaagccaga aattccaggg
      661 gttttaaggt attttaaagt gatatcatac aaagctgtac tgtcttattt ttgtgacaca
      721 atatattgcg tttgtcttat ttttgtgaca ctacatacag catttgtcac aaaaataaga
      781 cagtactttt ttataaaaaa tcaacaaaaa agacattagt caacactaat gtcttaaata
      841 ataattttgc ttttcgaaat tccaaattgc ggtaattgaa tgtgcttttt cttcttcgtc
      901 tgttgtttta tcttcatcac ttgcattaat caaattacca tcttcgacat cgtctaagtt
      961 taaaatttta tgtttttgtt taagcaatcc accataactt aacatacgtt ttcgatacaa
     1021 acctttttct aaatcactta caatttcttg atttttttca tcatcatcag ttaaaaaatc
     1081 agatgattta accgaatatt ttgaggtctc tttgattgcc gattcaatgt ctttatcgcc
     1141 ttttctattc ggtttgatcg ctttaacatt agcaacaggt cgatagtcaa cttgtaatgc
     1201 tctttgccat aaattaaccc attcttcttg agttatataa ttctcttttt ttctaaaata
     1261 tgcattttca acacataaca aaacatgcat gtgctgatta taactaccgt catttttatt
     1321 aacggtaact tctgttgaac gcataaatcc aacaagattt tgtttaacct ttttatatct
     1381 actcaaccta tcaaatgctt tagttagatg cttcaaactt tgttctaaag tatctccatc
     1441 tatcgcattt tttgttgaaa gtgttaaaaa caaccaacgt gcttttggct tttccttaat
     1501 tactttttca atcacttttt gagcttgata actatttttc atagcacgtc tccaattaca
     1561 aaccggacat aattttgatt tacaaaacca tgtcttatgt aacttcaaat agccttcatc
     1621 agttggctta aattctaaaa tattaccaca ttgttttaca ttaaaagcct ttttgatttt
     1681 taaaatttct agtatatctg catagcttac attatctatt ttcttttctc tccatgggcg
     1741 ttgtttccca cttttcgtca tatcttttaa cgttttatta tcttgatttt cggttatact
     1801 tctagtagta ttatattgca tataaaaaga cccccagtat tatttaagat tcgacacctt
     1861 aataatataa ctggggtttt ttattgtcaa attttccttt cacgcacaaa aacacgttta
     1921 aacactgtaa tatcaacagt tacagctata tatataaaat cgagttattt ctatatagta
     1981 tcaagacaag aagaaactcg ttcacactcg tttcaaaaaa caacctattc cttcttcttt
     2041 ttatgaccct ctaaacctaa ataaattaat gtgaaaacca atgaataaat aaaactttct
     2101 aaaaagtcaa aaggattatc aaataaaatc gaattaacaa aattaaaaat taaaaataca
     2161 gataataaaa taatcaaaaa gtaaaaaaac ttattcatat taaactcctt tgtttttata
     2221 tgttaatatt atattgaatt catttaaata acaaaacaag ggggcattta caatgaatgc
     2281 aattcaaaac agtatatcac taagaaagat tttagtagta tctttaagta ttttaaccat
     2341 actttcaatg gtcttggatt ttaatttcaa agaagctcaa gcccaaaaca aaaataacat
     2401 tgcagataaa aacgttgaaa ctttaaacga aaaagaaatt gaaaaagaac taaaatacat
     2461 atatggcaaa attattattt tagataaaga cggaacagct aaagatgtta atctagaaaa
     2521 tgccaaatct cgatatggat atgttcctga aggattccaa aaactaaaaa acgacataga
     2581 gaataaaaag aaaactagtt caatatcacc tagagcagtt ggtgggaact acaaaaatag
     2641 cggtgactgt ttctattctg aagttttaaa ctcatatgga gaacttttaa ctggaaacat
     2701 aatcacagca gtttttgaag atgttaaagc caaaaactta aagggcgtag caaaaaaatt
     2761 agctaaaatt gggataaaag gaaacctagc tggaatcgca gctactatgg tttcgaagga
     2821 tgtacaatgt aatcttaaat acgggttact ttagatacta tttattccaa ttgctttatt
     2881 gacgttgagc ctcggaaccc ttaacaatcc caaaacttgt cgaatggtcg gcttaatagc
     2941 tcacgctatg ccgacattcg tctgcaagtt tagttaaggg ttcttctcaa catcaataaa
     3001 ttttctcggc ataaatgcgt cgtctatttt ttattatctt catgataatc atatttttct
     3061 tccatctcgt caaaagcatc atctttatag aaaaacaaaa agtaagcaac aacaccaata
     3121 ggaat
//
LOCUS       DAKTIO010000227         3110 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.234, whole genome shotgun sequence.
ACCESSION   DAKTIO010000227 DAKTIO010000000
VERSION     DAKTIO010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.234"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            121..846
                     /locus_tag="O0293_002574"
     CDS             121..846
                     /locus_tag="O0293_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL14"
                     /protein_id="HCY0281428.1"
                     /translation="MKKNIMNKLVLSTALLLLETTSTQLPKTPISFSSEAKAYNISEN
                     ETNINELIKYYTQPHFSLSGKWLWQKPNGSIHATLQTWVWYSHIQVFGSESWGNINQL
                     RNKYVDIFGTKDEDTVEGYWTYDETFTGGVTPAATSSDKPYRLFLKYSDKQQTIIGGH
                     EFYKGNKPVLTLKELDFRIRQTLIKNKKLYNGEFNKGQIKITADGNNYTIDLSKELKL
                     TDTNRYVKNPRNAEIEVILEKSN"
     gene            941..1666
                     /locus_tag="O0293_002575"
     CDS             941..1666
                     /locus_tag="O0293_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL13"
                     /protein_id="HCY0281429.1"
                     /translation="MNNNITKKIILSTTLLLLGTASTQFPNTPINSSSEAKAYYINQN
                     ETNVNELTKYYSQKYLTFSNSTLWQKDNGTIHATLLQFSWYSHIQVYGPESWGNINQL
                     RNKSVDIFGIKDQETIDSFALSQETFTGGVTPAATSNDKHYKLNVTYKDKAETFTGGF
                     PVYEGNKPVLTLKELDFRIRQTLIKSKKLYNNSYNKGQIKITGADNNYTIDLSKRLPS
                     TDANRYVKKPQNAKIEVILEKSN"
     gene            1774..2490
                     /locus_tag="O0293_002576"
     CDS             1774..2490
                     /locus_tag="O0293_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL12"
                     /protein_id="HCY0281430.1"
                     /translation="MSKNITKNIILTTTLLLLGTVLPQNQKPVFSFYSEAKAYSIGQD
                     ETNINELIKYYTQPHFSFSNKWLYQYDNGNIYVELKRYSWSAHISLWGAESWGNINQL
                     KDRYVDVFGLKDKDTDQLWWSYRETFTGGVTPAAKPSDKTYNLFVQYKDKLQTIIGAH
                     KIYQGNKPVLTLKEIDFRAREALIKNKILYNENRNKGKLKITGGGNNYTIDLSKRLHS
                     DLANVYVKNPNKITVDVLFD"
     gene            complement(2935..>3110)
                     /locus_tag="O0293_002577"
     CDS             complement(2935..>3110)
                     /locus_tag="O0293_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281431.1"
                     /translation="NSLQSLFESESGYKISKNSGVPYQTVQDLRNGKTKLEDARFRTI
                     IKLYSYYVSLKEH"
BASE COUNT         1167 a          472 c          464 g         1007 t
ORIGIN      
        1 ttgcttaaat aacactacta actgtttaag tttatttaac atagttttag cttttattta
       61 attccgaatc ggtgtaatag cttatatact ttgggtaatt cacgcaaagg agattttcat
      121 atgaaaaaga acatcatgaa taaattagtt ttatcaacag cattgttact tttagaaact
      181 acatcaacac aacttcctaa aacaccaatc agtttttcat ctgaagcaaa agcctataat
      241 atcagtgaaa acgagactaa tatcaatgaa ctaatcaaat attacactca gccgcatttt
      301 tcattatctg gaaaatggtt atggcaaaag cccaatggta gcattcatgc aacattgcaa
      361 acgtgggttt ggtatagtca tattcaagtg tttggatccg agagttgggg aaacattaat
      421 cagttaagaa ataaatacgt tgatatattt ggaactaaag atgaggacac agttgaaggt
      481 tactggactt atgatgaaac atttactggt ggtgttacgc cagcagctac ttcatctgat
      541 aagccttata gactattttt aaaatatagt gataaacaac aaactatcat cggtggacat
      601 gaattttaca aaggaaataa accagtatta actttaaaag aattagattt ccgtattcgt
      661 caaacattaa taaaaaataa aaagttatat aacggagaat ttaataaagg tcaaattaag
      721 ataactgctg atggaaataa ttacacgatt gatttaagta aagagttaaa attaactgac
      781 acaaaccgtt atgttaaaaa tcctcgtaat gcagaaattg aagtcatact cgaaaaatct
      841 aactaaccta ttaccttttg taaatgcgga taatttcaat tatctaatta accccttttt
      901 ataattaaac attccaacaa tactcaaagg agaaattcga atgaacaata acatcacgaa
      961 aaaaattatt ttatcaacaa cattgttact attaggtaca gcatctacac aatttcctaa
     1021 tacacctatc aattcttcat ctgaagcgaa agcttattat ataaatcaaa acgaaactaa
     1081 cgttaatgag ttaactaaat attactcgca aaaatattta accttctcta acagtacgtt
     1141 atggcaaaaa gataacggta cgattcatgc aacgttgtta cagttttctt ggtatagtca
     1201 tattcaagtt tatggacctg aaagttgggg caatatcaac caattaagaa ataaaagcgt
     1261 tgatattttt ggcataaaag accaagaaac cattgattct tttgcattat ctcaagaaac
     1321 gtttactggt ggtgttactc ctgcagcaac atctaacgat aaacactata aactgaatgt
     1381 aacatataaa gataaagcag aaacgtttac tggcggattt ccagtttatg aaggcaataa
     1441 gcctgtttta actttaaaag aattagattt tcgtattcgt caaacattaa ttaaaagtaa
     1501 aaaattatat aataattctt ataataaagg acaaattaaa ataacaggtg cagacaataa
     1561 ctacacaata gatttaagta aaaggttgcc atcaactgat gcaaatagat atgttaaaaa
     1621 acctcaaaat gcaaaaattg aagttatcct cgaaaaatca aactaacaat aataatggag
     1681 ttaataaaaa taatcgcaaa tactatattg acttcgctca catttaaatt tcttattcct
     1741 cgtatcatga ttcctctgaa aggagatgtt ctaatgagta agaacatcac gaaaaatata
     1801 attttaacga caacattatt actattaggt actgtattac ctcaaaatca aaaaccagta
     1861 tttagttttt actctgaagc taaagcttat agcattggtc aagatgaaac taacatcaat
     1921 gaattaatta aatattacac acagcctcat ttttcatttt caaataaatg gctatatcaa
     1981 tatgataatg gaaacattta tgttgaactt aagagatatt catggtcagc acatatatct
     2041 ttatggggcg ctgaaagttg gggaaatatt aatcagttaa aagatcgtta cgtagatgtg
     2101 tttggactaa aagacaaaga tactgatcag ttatggtggt cttatagaga gacatttaca
     2161 ggtggcgtta caccagccgc aaaaccttct gataaaactt ataatctttt tgtgcaatac
     2221 aaagataaac tacaaacgat tattggtgcg cataaaatat accaaggcaa taaaccagta
     2281 ttaacattga aagaaatcga tttccgtgca cgagaagcgt taataaaaaa taaaatatta
     2341 tataacgaaa atcgtaataa aggtaagctt aagatcaccg gtggcggtaa taactacact
     2401 attgatttaa gcaaaagatt acattcagat ctagcaaatg tttatgttaa aaatcctaat
     2461 aaaataactg ttgacgtcct ctttgattag tatatgaagg tgacttatac ttcatgcact
     2521 ttaattccaa atcagattat ttaaatgata atttttaaag tgtatgatgt atataatagg
     2581 taaaattttc tatatattta aatggaattg ggagtaggaa tgtgacagaa atagtatttt
     2641 ataaaattta tttcgttgtc actccccaac ttgcacatta ttgtaagctg acttttcgtc
     2701 agcttctgtg ttggggccca ccccaacttg cattgtctgt agaatttctt tttgaaattc
     2761 tctatgttgg ggccccgcct ataattgaaa aatgcttgtt acatgggcat tttcattcgg
     2821 tcaactacta ccaatataat attgtagagc ctaagacatt gatttattat gtcttaggct
     2881 ctattccttc atttaatgat taaattatta tagcaatact ttattgtccc atgattagtg
     2941 ttcttttaat gagacatagt aactataaag tttaataatc gttctaaatc tagcatcttc
     3001 tagtttggtt ttcccatttc ttaaatcttg tactgtttga tatggaactc ctgaattttt
     3061 cgaaatttta tagcctgatt cagactcaaa caaagattga agagagttta
//
LOCUS       DAKTIO010000228         3060 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.235, whole genome shotgun sequence.
ACCESSION   DAKTIO010000228 DAKTIO010000000
VERSION     DAKTIO010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.235"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            106..1182
                     /locus_tag="O0293_002578"
     CDS             106..1182
                     /locus_tag="O0293_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCY0281432.1"
                     /translation="MNQSVKLLKHLTDVNGIAGYEMQVKEAMRNYIEPVSDQIIEDNL
                     GGIFGKKNAENGQYSIMISGHMDEVGFMVTKIDKHGFISFTPVGGWWNQVMLSQKVTI
                     TTDSGKEIRGIIGSKPPHVLTPEERKKPMEIKNMFIDIGVSSKEEAEEAGVEVGNMVT
                     PYSEFEVLANDKYLTAKAFDNRYGCALAIEVLKRLKDENIGINLYSGATVQEEVGLRG
                     AKVAANTIKPDLAIAVDVGIAYDTPGMSGQTSDSKLGGGPVVIMMDATSIAHQGLRKH
                     IKDVAKEHNIEVQWDTTPGGGTDAGSIHVANEGIPTMTIGVTLRYMHSNVSVLNVDDY
                     ENSIRLVTEIVRSLNDESYKNIMW"
     gene            1430..2092
                     /locus_tag="O0293_002579"
     CDS             1430..2092
                     /locus_tag="O0293_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0281433.1"
                     /translation="MLLEIKDLVYKASDRIILDHISLKVDKGESIAIIGPSGSGKSTF
                     QKQICNLFSPTSGELYFKGKPYNDYDPEELRQRISYLMQQSDLFGETIEDNMIFPSLA
                     RNDKFDRKRAKQLIKDVGLGHYQLSSEVENMSGGERQRIAIARQLMYTPDILLLDEST
                     SALDVNNKEKIENIIFKLADQGVAIMWITHSDDQSMRHFQKRITIVDGQISNTEELNQ
                     HE"
     gene            2085..2861
                     /gene="fetB"
                     /locus_tag="O0293_002580"
     CDS             2085..2861
                     /gene="fetB"
                     /locus_tag="O0293_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCY0281434.1"
                     /translation="MSNTALGLTALLLVIPIIISYKEGLHIIKDLIVATLRAVVQLII
                     LGFLLHYIFKINDKWLLILCVLVIIINASWNTISRASPVMHHVFWISFLAIFIGTALP
                     LAGTIATGAIQFTANEVIPIGGMLANNGLIAINLAYQNLDRAFVQDGTNIESKLSLAA
                     TPKLASKGAIRESIRLAIVPTIDSVKTYGLVSIPGMMTGLIIGGVPPLQAIKFQLLVV
                     FIHTTATIMSALIATYLSYGQFFNARHQLVARNTDVKSES"
BASE COUNT         1032 a          432 c          634 g          962 t
ORIGIN      
        1 aattttaaaa atatttaagc ctgaaaatga taaagcggta gggaacgttt ttctgaaagt
       61 tagtgataca atagttttaa gttgaaatac aggaggatga ataacatgaa tcagtcagtc
      121 aaattactta aacatttaac agatgtaaac ggcattgctg gttatgaaat gcaagttaaa
      181 gaagcaatgc gtaactatat agagcctgtc agtgatcaaa ttattgaaga taacttgggt
      241 ggcatttttg gaaagaaaaa tgctgagaat ggtcaatact caattatgat ttctggtcat
      301 atggatgaag ttggttttat ggtaacaaag attgataaac atggttttat ttcatttacg
      361 ccagttggtg gatggtggaa tcaagtcatg ctatctcaaa aagtaacgat tacaacagat
      421 tcgggcaaag aaattagagg tatcatcggt tctaaaccgc cacatgtctt aacgcctgaa
      481 gaacgtaaaa agccaatgga aatcaaaaat atgtttatag atattggtgt tagtagcaag
      541 gaagaagctg aagaagctgg cgttgaagta ggcaatatgg ttacgccata tagtgaattt
      601 gaagtgcttg caaatgataa atatttaact gcgaaagcat ttgataatcg ctatggctgt
      661 gcattagcta ttgaggtatt aaaacgttta aaagatgaaa atattggcat taacttatac
      721 agtggtgcca cagtgcaaga agaagttggt ttgcgtggtg cgaaagtggc agcgaatacg
      781 attaaaccag acttggcgat agctgtcgat gtaggtattg cttatgatac cccaggtatg
      841 tcaggtcaaa cgagcgatag taaactaggc ggtggtccag ttgtcattat gatggatgct
      901 acaagtattg ctcaccaagg tttgcgaaag catattaaag atgtagctaa ggaacataac
      961 atcgaagtac aatgggatac gacaccaggt ggaggtacag atgcgggaag tattcatgtc
     1021 gcaaatgaag gtattccaac gatgacaatc ggtgttacgc tgcgatacat gcattctaat
     1081 gtttcagtgc tcaatgtaga tgattatgaa aattctatcc gtcttgttac tgaaattgtc
     1141 cgttcattga atgatgaaag ttataaaaat atcatgtggt aatcaaatcc ataaataata
     1201 aagaatcctt ttaatatggt aggttgttaa acaattgtct aattttaatt cttagtcatt
     1261 agacagtatc catgttaata ggattttttg tttttaattt aaatgctgaa aatcaattat
     1321 gcctaaattt tgatattaca agaaaatgat tttttcttaa atgtaattgc actaaaaacc
     1381 aaaaaaacgg gaataatata cctgatatat tacatgagga gcggtgcaaa tgttgttaga
     1441 aattaaagat ttagtgtata aagcgagcga tagaatcata ctagatcata tcagtctaaa
     1501 agtagataaa ggcgagagta ttgccattat aggtccatca ggtagtggta aaagtacatt
     1561 tcaaaagcaa atatgtaatt tgtttagtcc aactagtgga gaactttatt ttaaaggtaa
     1621 accctataat gattatgacc cggaagaatt gcgtcaacga atcagttatt tgatgcagca
     1681 aagtgacttg tttggtgaaa cgattgaaga taacatgata ttcccatcac ttgcacgtaa
     1741 tgataaattt gatagaaaac gtgcaaagca attaattaaa gatgtcggtt tgggacatta
     1801 tcaattaagt tcggaagtgg aaaatatgtc gggtggtgag cggcaaagaa ttgctatagc
     1861 gcgccaactg atgtatacac cggatattct tttattagat gaatcgacca gtgcattaga
     1921 cgttaataat aaagaaaaga tagaaaatat catttttaaa ttagcagatc aaggcgtggc
     1981 aattatgtgg attacccaca gcgatgacca aagtatgcga cactttcaaa agcgtataac
     2041 aattgttgat ggtcaaattt ctaatacaga ggagttgaat caacatgagt aatacggcac
     2101 ttggattgac ggcattgctt ttagtcattc cgattatcat ttcatataaa gaaggtttac
     2161 atattattaa agatttaatt gttgcgacat tacgagcagt tgtgcaatta atcattttgg
     2221 gatttttgct gcattatatt tttaaaataa acgataaatg gctgcttatt ttatgtgtat
     2281 tggtcattat tattaatgca tcatggaata caattagtcg agcatcacca gtgatgcatc
     2341 atgtgttttg gatatcattt ctagctatct tcattggaac ggcattaccg cttgcaggta
     2401 ctattgcgac aggggccatt caatttaccg caaatgaagt tatacctatc ggcggcatgc
     2461 ttgcaaataa tggcttgatt gcaattaatt tagcttacca gaatttagat cgtgcattcg
     2521 tacaagatgg tactaatatt gaatctaaat tatcacttgc agctacacct aaattggctt
     2581 ctaaaggtgc aatacgtgaa agtattcgtt tagctatagt gccaactatt gattcggtta
     2641 aaacatatgg gcttgtgtcg attcctggta tgatgacagg cttaattatt ggtggcgtac
     2701 cacctttaca agcgattaaa tttcaattgt tagtcgtgtt tattcataca actgcgacca
     2761 ttatgtctgc tttgattgcg acatatttaa gctatggtca atttttcaat gcaagacatc
     2821 aattagtagc acgaaatact gatgttaaga gtgaatcatg atagatttta ctgcatcaga
     2881 tttaggcatt agttttaatt ggaaatgaag tgacgcgcac atatagtatc gctattcatt
     2941 agcgcagcga aaatattcat aaaggcacgc atactttgta gtcagttatc tgttctgaca
     3001 tataaagcgt gcgtgctttt ttggagttat tgttgaaact gaagtaatta tacataatta
//
LOCUS       DAKTIO010000229         3041 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.236, whole genome shotgun sequence.
ACCESSION   DAKTIO010000229 DAKTIO010000000
VERSION     DAKTIO010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.236"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(276..2885)
                     /gene="adhE"
                     /locus_tag="O0293_002581"
                     /gene_synonym="adhC"
     CDS             complement(276..2885)
                     /gene="adhE"
                     /locus_tag="O0293_002581"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCY0281435.1"
                     /translation="MLTIPEKENRGSKEQEVAIMIDALADKGKKALEALSKKSQEEID
                     HIVHQMSLAAVDQHMVLAKLAHEETGRGIYEDKAIKNLYASEYIWNSIKDNKTVGIIG
                     EDKEKGLTYVAEPIGVICGVTPTTNPTSTTIFKAMIAIKTGNPIIFAFHPSAQESSKR
                     AAEVVLEAAMKAGAPKDIIQWIEVPSIEATKQLMNHKGIALVLATGGSGMVKSAYSTG
                     KPALGVGPGNVPSYIEKTAHIKRAVNDIIGSKTFDNGMICASEQVVVIDKEIYKDVTN
                     EFKAHQAYFVKKDELQRLENAIMNEQKTGIKPDIVGKSAVEIAELAGIPVPENTKLII
                     AEISGVGSDYPLSREKLSPVLALVKAQSTKQAFQICEDTLHFGGLGHTAVIHTEDETL
                     QKDFGLRMKACRVLVNTPSAVGGIGDMYNELIPSLTLGCGSYGRNSISHNVSATDLLN
                     IKTIAKRRNNTQIFKVPAQIYFEENAIMSLTTMDKIEKVMIVCDPGMVEFGYTKTVEN
                     VLRQKTEQPQIKIFSEVEPNPSTNTVYKGLEMMVDFQPDTIIALGGGSAMDAAKAMWM
                     FFEHPETSFFGAKQKFLDIGKRTYKIGMPENATFICIPTTSGTGSEVTPFAVITDSET
                     NVKYPLADFALTPDVAIIDPQFVMSVPKSVTADTGMDVLTHAMESYVSVMASDYTRGL
                     SLQAIKLTFEYLKSSVEKGDKVSREKMHNASTLAGMAFANAFLGIAHSIAHKIGGEYG
                     IPHGRANAILLPHIIRYNAKDPQKHALFPKYEFFRADTDYADIAKFLGLKGNTTEALV
                     ESLAKAVYELGQSVGIEMNLKSQGVSEEELNESIDRMAELAFEDQCTTANPKEALISE
                     IKDIIQTSYDYKQ"
BASE COUNT          874 a          570 c          472 g         1125 t
ORIGIN      
        1 tttgaacaat taattacttt taaaccgcat tattttcgcc tttttattta tatgaaatta
       61 aaaataatta tactctaaag tagtaatagt ttgtgaaatg agtatcaatc ttaatatttt
      121 ctttatttaa aaaaatgatt gtctagtttg tatctctctg aagatttggc aataaataaa
      181 agccgataac cgtataatga ttatcgactt aaagtttatg tggcattttt tacttttgta
      241 atttcaggtg agttagatga ttattatcag atagattatt gcttataatc atatgatgtt
      301 tgaatgatat ctttgatttc actgattagt gcttctttag gattagcagt tgtacattga
      361 tcttcaaatg cgagctctgc cattctatca attgactcat ttaattcttc ttcagacaca
      421 ccttgtgatt tcaaattcat ttcaattccg actgattgac ctaattcgta gacagcttta
      481 gctaatgatt ctacgagtgc ttctgtcgta ttacctttta atcctaagaa tttggcaata
      541 tctgcataat ctgtatctgc tctgaagaac tcatatttag ggaataatgc atgtttttgc
      601 gggtctttgg cattataacg gataatatgc ggtagtaata tcgcattcgc tctaccatgc
      661 ggaataccat attcgccacc aattttatgc gcaattgagt gtgcaatgcc taagaatgca
      721 tttgcaaatg ccataccagc caaagttgat gcgttatgca ttttctctct tgaaacttta
      781 tcaccctttt caacagatga ttttaaatat tcgaacgtca atttaatcgc ttgtagactc
      841 aaacctcttg tgtagtctga agccattaca gatacatatg attccattgc atgcgttagt
      901 acatccattc ctgtatctgc tgtaacgctt tttggcacac tcatcacaaa ttgagggtca
      961 ataattgcaa cgtcaggtgt taaagcaaaa tcagccaacg gatattttac atttgtttca
     1021 ctatctgtga taactgcaaa tggtgttact tctgaacctg tacctgatgt cgtagggata
     1081 caaatgaacg tcgcattttc aggcatgcct attttataag tacgtttacc gatgtctagg
     1141 aacttttgtt tagcaccgaa gaatgatgtc tcagggtgtt caaagaacat ccacattgct
     1201 tttgcagcat ccatcgctga accaccacca agtgcaatga ttgtatccgg ttggaaatca
     1261 accatcattt ccagaccttt atatactgta ttagttgatg ggttcggttc gacttcgcta
     1321 aatattttaa tttgaggctg ttccgttttt tgtcttaata cattctcaac tgtttttgta
     1381 taaccgaatt ctaccatacc agggtcacag acaatcatca ctttttcaat cttgtccatt
     1441 gttgttagac tcatgattgc attttcttca aaataaattt gagcaggcac cttgaaaatt
     1501 tgagtattat tacgtcgttt agcaatcgtt ttaatgttta ataaatctgt cgcactaaca
     1561 ttatgtgaaa ttgagtttct accgtaggaa ccacaaccta atgttaaaga cggaatcaat
     1621 tcgttataca tatcaccaat acctccaacc gctgatggtg tatttacaag tacacgacaa
     1681 gctttcattc ttagtccaaa atctttttgt aatgtttcat cttctgtatg gataacggct
     1741 gtgtgtccta atccaccaaa atgtagtgtg tcttcacaaa tttgaaatgc ttgttttgta
     1801 gattgggctt ttactaaggc taatactgga gataattttt cacgagataa cggatagtct
     1861 gaacctacac cgctaatttc ggctatgata agttttgtat tttcggggac aggtatacct
     1921 gctaattcag ctatttcaac tgcagattta ccgacaatat caggcttaat acctgttttt
     1981 tgttcattca taattgcatt ttctaagcgt tgtaattcat cttttttaac aaagtatgct
     2041 tgatgtgctt taaattcatt agtaacatct ttataaattt ctttatcaat gactacaact
     2101 tgttcagaag cacaaatcat accattatca aatgtttttg aaccaatgat atcatttact
     2161 gcacgtttaa tgtgtgctgt tttttcaatg taagacggca cgttacctgg tcccacacct
     2221 aatgccggtt tgccagttga atatgcagac ttaaccatgc ccgaaccacc tgttgctaga
     2281 actaatgcaa tacctttgtg attcattaat tgttttgttg cttcgataga aggcacttca
     2341 atccactgaa taatatcttt aggtgcacct gccttcattg ccgcttctaa tacaacttct
     2401 gctgcacgct tcgacgattc ttgtgcactt ggatggaatg caaaaatgat tggatttcct
     2461 gtcttaattg caatcatcgc tttaaaaata gttgtcgacg taggatttgt tgttggcgta
     2521 acaccacaaa taacaccaat tggttccgct acatacgtta atcctttttc tttatcttca
     2581 ccaataatcc ctactgtctt attgtctttt attgaattcc atatatattc agaagcgtat
     2641 aaatttttaa tcgctttatc ttcgtatata cctcttccag tttcttcatg tgctaatttt
     2701 gctagcacca tatgttgatc aacagctgct aagctcattt gatgaacaat atgatcaatt
     2761 tcttcttgtg actttttaga taatgcttct aatgcttttt tccctttgtc agctagagca
     2821 tcaatcataa ttgccacttc ttgttctttc gatccacgat tttctttttc aggtatagtt
     2881 aacatataca accactcctt tatactttgt gaattatttc acaaacatta tagtacatgt
     2941 ctctcaggat ataaagaaaa ttctatacaa aaaagtttaa tttcgaatat tatttgaaca
     3001 aatatcaaat tttaaaataa atgttttcat gaaatcattg t
//
LOCUS       DAKTIO010000230         2990 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.237, whole genome shotgun sequence.
ACCESSION   DAKTIO010000230 DAKTIO010000000
VERSION     DAKTIO010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.237"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            15..2858
                     /gene="sdrC"
                     /locus_tag="O0293_002582"
     CDS             15..2858
                     /gene="sdrC"
                     /locus_tag="O0293_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrC"
                     /protein_id="HCY0281436.1"
                     /translation="MNNKKTATNRKGMIPNRLNKFSIRKYSVGTASILVGTTLIFGLS
                     GHEAKAAEHTNGELNQSKNETTAPSENKTTKKVDSRQLKDNTQTATADQPKVTMSDSA
                     TVKETSSNMQSPQNATANQSTTKTSNVTTNDKSSTTYSNETDKSNLTQAKDVSTTPKT
                     TTIKPRTLNRMAVNTVAAPQQGTNVNDKVHFSNIDIAIDKGHVNQTTGKTEFWATSSD
                     VLKLKANYTIDDSVKEGDIFTFKYGQYFRPGSVRLPSQTQNLYNAQGNIIAKGIYDST
                     TNTTTYTFTNYVDQYTNVRGSFEQVAFAKRKNATTDKTAYKMEVTLGNDTYSEEIIVD
                     YGNKKAQPLISSTNYINNEDLSRNMTAYVNQPKNTYTKQTFVTNLTGYKFNPNAKNFK
                     IYEVTDQNQFVDSFTPDTSKLKDVTDQFDVIYSNDNKTATVDLMKGQTSSNKQYIIQQ
                     VAYPDNSSTDNGKIDYTLDTDKTKYSWSNSYSNVNGSSTANGDQKKYNLGDYVWEDTN
                     KDGKQDANEKGIKGVYVILKDSNGKELDRTTTDENGKYQFTGLSNGTYSVEFSTPAGY
                     TPTTANVGTDDAVDSDGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQ
                     DSTEKGIKGVKVTLQNEKGEVIGTTETDENGKYRFDNLDSGKYKVIFEKPAGLTQTGT
                     NTTEDDKDADGGEVDVTITDHDDFTLDNGYYEEETSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDNDSDSDSDSDSDAGKHTPAKPMSTVKDQHKTAKALPETG
                     SENNNSNNGTLFGGLFAALGSLLLFGRRKKQNK"
BASE COUNT         1190 a          491 c          539 g          770 t
ORIGIN      
        1 aggagtatta ttaaatgaat aataaaaaga cagcaacaaa tagaaaaggc atgataccaa
       61 atcgattaaa caaattttcg ataagaaagt attctgtagg tactgcttca attttagtag
      121 ggacaacatt gatttttggg ttaagtggtc atgaagctaa agcggcagaa catacgaatg
      181 gagaattaaa tcaatcaaaa aatgaaacga cagccccaag tgagaataaa acaactaaaa
      241 aagttgatag tcgtcaacta aaagacaata cgcaaactgc aactgcagat cagcctaaag
      301 tgacaatgag tgatagtgca acagttaaag aaactagtag taacatgcaa tcaccacaaa
      361 acgctacagc taatcaatct actacaaaaa ctagcaatgt aacaacaaat gataaatcat
      421 caactacata tagtaatgaa actgataaaa gtaatttaac acaagcaaaa gatgtttcaa
      481 ctacacctaa aacaacgact attaaaccaa gaactttaaa tcgcatggca gtgaatactg
      541 ttgcagctcc acaacaagga acaaatgtta atgataaagt acatttttca aatattgaca
      601 ttgcgattga taaaggacat gttaatcaga ctactggtaa aactgaattt tgggcaactt
      661 caagtgatgt tttaaaatta aaagcaaatt acacaatcga tgattctgtt aaagagggcg
      721 atatatttac ttttaaatat ggtcaatatt tccgtccagg atcagtaaga ttaccttcac
      781 aaactcaaaa tttatataat gcccaaggta atattattgc aaaaggtatt tatgatagta
      841 caacaaacac aacaacatat acttttacga actatgtaga tcaatataca aatgttagag
      901 gtagctttga acaagttgca tttgcgaaac gtaaaaatgc aacaactgat aaaacagctt
      961 ataaaatgga agtaacttta ggtaatgata catatagcga agaaatcatt gtcgattatg
     1021 gtaataaaaa agcacaaccg cttatttcaa gtacaaacta tattaacaat gaagatttat
     1081 cgcgtaatat gactgcatat gtaaatcaac ctaaaaatac atatactaaa caaacgtttg
     1141 ttactaattt aactggatat aaatttaatc caaatgcaaa aaacttcaaa atttacgaag
     1201 tgacagatca aaatcaattt gtggatagtt tcacccctga tacttcaaaa cttaaagatg
     1261 ttactgatca attcgatgtt atttatagta atgataataa aacagctaca gtcgatttaa
     1321 tgaaaggcca aacaagcagc aataaacaat acatcattca acaagttgct tatccagata
     1381 atagttcaac agataatgga aaaattgatt atactttaga cactgacaaa actaaatata
     1441 gttggtcaaa tagttattca aatgtgaatg gctcatcaac tgctaatggc gaccaaaaga
     1501 aatataatct aggtgactat gtatgggaag atacaaataa agatggtaaa caagatgcca
     1561 atgaaaaagg gattaaaggt gtttatgtca ttcttaaaga tagtaacggt aaagaattag
     1621 atcgtacgac aacagatgaa aatggtaaat atcagttcac tggtttaagc aatggaactt
     1681 atagtgtaga gttttcaaca ccagccggtt atacaccgac aactgcaaat gtaggtacag
     1741 atgatgctgt agattctgat ggactaacta caacaggtgt cattaaagac gctgacaaca
     1801 tgacattaga tagtggattc tacaaaacac caaaatatag tttaggtgat tatgtttggt
     1861 acgacagtaa taaagatggt aaacaagatt cgactgaaaa aggaattaaa ggtgttaaag
     1921 ttactttgca aaacgaaaaa ggcgaagtaa ttggtacaac tgaaacagat gaaaatggta
     1981 aataccgctt tgataattta gatagtggta aatacaaagt tatctttgaa aaacctgctg
     2041 gcttaactca aacaggtaca aatacaactg aagatgataa agatgccgat ggtggcgaag
     2101 ttgatgtaac aattacggat catgatgatt tcacacttga taatggctac tacgaagaag
     2161 aaacatcaga tagcgactca gattctgaca gcgattcaga ctcagatagc gactcagatt
     2221 cagatagcga ctcagattca gacagcgatt cagacagcga ctcagactca gatagcgatt
     2281 cagattcaga cagcgactca gactcagaca gcgattcaga ctcggatagc gactcagact
     2341 cagatagcga ctcagattcg gatagcgact cagactcaga tagcgattca gattcagata
     2401 gcgattcgga ctcagacagt gattcagatt cagactcaga tagcgactca gattctgaca
     2461 gcgattcaga ctcagacagc gactcagact cagacagtga ttcagattca gacagcgact
     2521 cagattcaga tagcgactca gactcagata gcgactcaga ctcagatagc gactcagact
     2581 cggatagcga ttcagattca gacagcgact cagattcaga tagcgattcg gactcagaca
     2641 acgactcaga ttcagatagc gattcagatt cagatgcagg taaacatact ccggctaaac
     2701 caatgagtac ggttaaagat cagcataaaa cagctaaagc attaccagaa acaggtagtg
     2761 aaaataataa ttcaaataat ggcacattat tcggtggatt attcgcggca ttaggatcat
     2821 tattgttatt cggtcgtcgt aaaaaacaaa ataaataata tgattaactt aaccaggtcc
     2881 atgtggcctg gttttttctg tttagaaatt cccagatata aaacaaaaaa tctttaagga
     2941 atagatacaa tttaatttgt tacagaaatg taattgtcta tgaattagaa
//
LOCUS       DAKTIO010000231         2989 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.238, whole genome shotgun sequence.
ACCESSION   DAKTIO010000231 DAKTIO010000000
VERSION     DAKTIO010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.238"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            413..1111
                     /locus_tag="O0293_002583"
     CDS             413..1111
                     /locus_tag="O0293_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL9"
                     /protein_id="HCY0281437.1"
                     /translation="MKLTTIAKATLALGILTTGVFTAESQTGHAKVELDETQRKYYIN
                     MLHQYYSEESFEPTNISVKSEDYYGSNVLNFKQRNKAFKVFLLGDDKNKYKEKTHGLD
                     VFAVPELIDIKGGIYSVGGITKKNVRSVFGFVSNPSLQVKKVDAKNGFSINELFFIQK
                     EEVSLKELDFKIRKLLIEKYRLYKGTSDKGRIVINMKDEKKHEIDLSEKLSFERMFDV
                     MDSKQIKNIEVNLN"
     gene            1477..2160
                     /locus_tag="O0293_002584"
     CDS             1477..2160
                     /locus_tag="O0293_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL10"
                     /protein_id="HCY0281438.1"
                     /translation="MKFTALAKATLALGILTTGTLTTEVHSGHAKQNQKSVNKHDKEA
                     LYRYYTGKTMEMKNISALKHGKNNLRFKFRGIKIQVLLPGNDKSKFQQRSYEGLDVFF
                     VQEKRDKHDIFYTVGGVIQNNKTSGVVSAPILNISKEKGEDAFVKGYPYYIKKEKITL
                     KELDYKLRKHLIEKYGLYKTISKDGRVKISLKDGSFYNLDLRSKLKFKYMGEVIESKQ
                     IKDIEVNLK"
     gene            complement(2245..2346)
                     /locus_tag="O0293_002585"
     CDS             complement(2245..2346)
                     /locus_tag="O0293_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281439.1"
                     /translation="MENFDVGGGNFTFMEIGIGFMTHKPKRLNDDID"
     gene            2424..>2989
                     /locus_tag="O0293_002586"
     CDS             2424..>2989
                     /locus_tag="O0293_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HCY0281440.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPEDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFL"
BASE COUNT         1193 a          383 c          526 g          887 t
ORIGIN      
        1 caaattaaaa acatcgaagt gaatttgaaa taatcaatga tatatataga atgaaagctt
       61 aagaagcggt ttaataatcc catgtttaat gattttgata cgtgttttta taataaaaac
      121 atatcgaaca ttgactacgt tattaagctg cttttttgta cactttataa ccaatagctt
      181 aagatttaaa actaatcgga aagaacaatg attcaccaaa aaaatattta tgttgctatt
      241 aaaaatcagt taatacgaat gttaaaatac gtttgatttt cattaataat gattcaagtt
      301 tatttaaatg agcgttaatg tcagtctgtt ttgatgcacc ttataataaa gacagatagt
      361 tcaaattacg taataataac aatccaatac attaagattg gagcaaaaaa atatgaaatt
      421 aacaacgata gctaaagcaa cattagcatt aggaatatta actacaggtg tgtttacagc
      481 agaaagtcaa actggtcacg cgaaagtaga acttgatgag acacaacgca aatattatat
      541 caatatgcta catcaatact attctgaaga aagttttgaa ccaacaaaca ttagtgttaa
      601 aagcgaagat tactatggct ctaacgtttt aaactttaaa caacgaaata aagcttttaa
      661 agtattttta cttggtgacg ataaaaataa atataaagaa aaaacacatg gccttgatgt
      721 ctttgcagta cctgaattaa tagatataaa aggtggcata tatagcgttg gcggtataac
      781 aaagaaaaat gtgagatcag tgtttggatt tgtaagtaat ccaagtctac aagttaaaaa
      841 agttgatgct aaaaatggct tttcgataaa cgagttgttt tttattcaaa aggaagaagt
      901 atcattgaag gaactggact ttaaaataag aaaactctta atcgaaaaat atagattgta
      961 taaaggaacg tctgataaag gtagaattgt tatcaatatg aaagacgaaa agaagcatga
     1021 aattgattta agtgaaaaat taagttttga acgtatgttt gatgtaatgg atagtaagca
     1081 aattaaaaat attgaagtga atttgaatta gttcgagtta atagcataat agcttaagaa
     1141 gcggcttaac gacaaaatgt gaattggcat ttgtgtcctt atataaggaa ctgtgttaaa
     1201 tacattactg ttgttaagtt gtttttgtaa ttcaaagagc agaacagagt aacatcatca
     1261 gttgtagtaa acgataatcc ggtaaaacaa ctaaatgaaa taatgaaagt catttaacct
     1321 gaacattaaa atatatttgt ttttcattaa gaataattca agtatattta aatcgaggtt
     1381 aattatcgta tgaaacgatg cacgttataa taaaaatgta tgattcaaat tacgtaatga
     1441 aaacaatcca atatattaag attggagcaa ataaatatga aatttacagc attagcaaaa
     1501 gcgacattag ctttaggaat tttaacaaca ggaactttaa caacagaagt tcattcaggt
     1561 catgcaaaac aaaatcaaaa gtcagtaaat aaacatgaca aggaagcatt ataccgatac
     1621 tacactggaa agactatgga aatgaaaaat attagtgctt tgaaacatgg taaaaacaac
     1681 ttacgtttta agtttagagg tattaagatt caagttttac tgcctggaaa tgataaaagt
     1741 aaatttcaac agcgtagtta tgaggggtta gatgttttct ttgttcaaga aaaaagagat
     1801 aagcacgata tattttatac tgttggtggt gtaatacaga ataataaaac atctggagtt
     1861 gtcagtgcac caatattaaa tatttcaaaa gaaaagggtg aagatgcttt tgtgaaaggt
     1921 tacccttatt acattaaaaa agaaaaaata acactaaaag aactggatta taagttgaga
     1981 aagcatctaa ttgaaaaata cggactttat aaaacaatct caaaagatgg tagggtcaaa
     2041 attagcttga aagatggcag tttttataac cttgatttaa gatctaaatt aaaatttaaa
     2101 tatatggggg aagtcataga aagcaaacaa attaaagata ttgaagttaa cttaaagtaa
     2161 atcattacga ataattaaaa gtaattgaag cggcttaacg gtgaaatgta aattggtgcg
     2221 catagcttat acaaaaagga tgcatcaatc gatatcgtcg ttaagccgtt ttggtttgtg
     2281 tgtcatgaat cctatcccaa tctccataaa ggtaaaattt ccaccaccaa catcaaaatt
     2341 ctccacatcg caacataacc aaatgttata ataaatctat tacacaaaga gataaattac
     2401 ttattcaaag gcggaggaat cacatgtcta ttactgaaaa acaacgtcag caacaagctg
     2461 aattacataa aaaattatgg tcgattgcga atgatttaag agggaatatg gatgcgagtg
     2521 aattccgtaa ttacatttta ggcttgattt tctatcgctt cttatctgaa aaagcggaac
     2581 aagaatatgc agatgccttg tcaggtgaag acatcacgta tcaagaagca tgggcagacg
     2641 aagaataccg tgaagactta aaagcagaat taattgacca agtcggttac ttcattgagc
     2701 cagaagattt attcagtgcg atgattcgtg aaattgaaac gcaagatttc gatatcgaac
     2761 acctggcgac ggcaattcgt aaagttgaaa catcaacatt aggtgaagaa agtgaaaatg
     2821 actttatcgg tctgttcagc gatatggatt tgagttcaac gcgactaggt aacaatgtca
     2881 aagaacgtac tgctttaatc tctaaagtca tggttaatct tgacgactta ccattcgttc
     2941 acagtgacat ggaaattgat atgttaggtg atgcatatga attcctaat
//
LOCUS       DAKTIO010000232         2957 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.239, whole genome shotgun sequence.
ACCESSION   DAKTIO010000232 DAKTIO010000000
VERSION     DAKTIO010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.239"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(117..776)
                     /locus_tag="O0293_002587"
     CDS             complement(117..776)
                     /locus_tag="O0293_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281441.1"
                     /translation="MKRTDKYRDSYQYDNQNQNHRRQSEDASYRQQYAKGDPEEHPER
                     YYNGRDYRREQILEEENEKSRRSKKWLYIIIAILLIIVAIFVTRALLNNDSDKVSNDP
                     KVSQNYKKQVENQDGQINQQVDNAKENIKNNQKTDDIIKNLQNQIDNLKQQEQNKADS
                     KLTQFYQDQINKLTEANNALKNNASQGKIESMLNDINTKFDSIKSKLESLFKDDNGGA
                     N"
     gene            complement(836..1978)
                     /locus_tag="O0293_002588"
     CDS             complement(836..1978)
                     /locus_tag="O0293_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCY0281442.1"
                     /translation="MQLQKIVIAPDSFKESMTAQQVGNIIKQAFTNVYGNTLHYDIIP
                     MADGGEGTTDALMHATGATKYTVIVNDPLMRPIEACYARADEQQIAIIEMAAASGLDL
                     LEKEERNPLYTSSYGTGELIKDALNHGAKTIILGIGGSATNDGGTGMLSALGVKFTDV
                     NGDLLQMNGANLAHIAQIDITNLDSRLKEVTFKVACDVSNPLLGENGATYIYGPQKGA
                     DAKMIPKLDFAMSHYHDKIKMCTGKSVNQIPGSGAAGGMGAALLAFCETTLTKGIDVV
                     FDITDFHQRIKDADLVITGEGRMDYQTIFGKTPVGVALAAKQYHIPVIAICGSLGENY
                     QHVYDFGIDSAYSIISSPSTLEDVLQNSEQNLLNTATDIARILKLQ"
     gene            2246..2632
                     /gene="gtxA"
                     /locus_tag="O0293_002589"
     CDS             2246..2632
                     /gene="gtxA"
                     /locus_tag="O0293_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase GtxA"
                     /protein_id="HCY0281443.1"
                     /translation="MKLTQTHAEILKFIIVGGINTLNYYVVYLLLLKLLHIEYMISHI
                     TGFLVAFVISYYLNCYFVYRVKPTWRKFISFPITQIVNVSLQTVLLYVFVSWLNLPAE
                     IAPFAGLIITIPITFILSKWILKDSN"
     gene            2766..2873
                     /locus_tag="O0293_002590"
     CDS             2766..2873
                     /locus_tag="O0293_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCY0281444.1"
                     /translation="MFNLLINIMTSAISGCLVAFFAHWLRTRNNKKGDK"
BASE COUNT          890 a          496 c          516 g         1055 t
ORIGIN      
        1 ttagaataca agtttatata aaaaatccct atactcttaa tgcatcatca cgaataacgt
       61 agtgatacag ataaagtata gggaattaat gtttatcatc aaagcaggtg taataattaa
      121 ttagcgccac cattgtcatc tttaaataag ctttctaatt tagatttaat actgtcgaat
      181 tttgtattaa tatcatttaa catgctttca attttacctt ggcttgcatt gtttttaagt
      241 gcattatttg cctctgtcaa tttgttgatt tggtcttgat aaaattgagt tagcttagaa
      301 tcagctttgt tttgttcttg ctgcttcaag ttgtcgattt gattttgtaa atttttaata
      361 atgtcatcag ttttttggtt gtttttaata ttttctttag cattatctac ttgctggtta
      421 atttggccgt cttgattttc aacttgtttt ttataatttt gagagacttt agggtcatta
      481 ctaactttat cgctatcatt gttaagtaag gcgcgtgtga caaaaatagc gacaataatt
      541 aagagaatgg caataatgat atataaccat ttttttgaac ggcgggattt ctcgttttct
      601 tcttcaagaa tttgttctct tcgataatct ctaccattat agtatcgttc cgggtgttct
      661 tcaggatcgc ctttagcata ttgttgtcta tacgatgcgt cttcagattg acgacgatga
      721 ttttggtttt gattgtcgta ttgatatgaa tcacggtatt tatcagttct tttcatgggt
      781 ttcacctcat tcaagtttta actgcaaata ataaagctga tgatttactt tgacattatt
      841 gtaatttcag aatacgagca atgtcagttg cagtgtttaa taaattttgt tcgctatttt
      901 gtaggacatc ttctaaagtg ctaggtgaag agattataga ataggcacta tcaataccga
      961 aatcgtaaac atgttgataa ttttcgccta gactgccaca aatcgcgatg acaggaatat
     1021 gatattgttt tgcagctaac gcaacgccta cgggtgtttt accaaagatg gtctgataat
     1081 ccatgcgtcc ttctccagta ataacgaggt ctgcatcttt aattctttga tgaaaatctg
     1141 taatgtcaaa gacgacatca ataccttttg ttaaagttgt ctcacaaaac gctaataatg
     1201 ctgcgcccat accgccagct gcaccagaac ctggtatttg attaacggac tttcctgtgc
     1261 acatttttat cttatcatga taatgcgaca ttgcgaaatc caactttggt atcatctttg
     1321 catcagcgcc tttttgagga ccataaatat aggtagcacc attttcaccc aataaaggat
     1381 ttgaaacatc acaggccact ttaaaggtca cctcttttaa tcgcgaatct agattggtta
     1441 tatcgatttg tgcaatgtga gcaagattag caccattcat ttgtaataag tccccgttta
     1501 catcagtaaa ctttacgcct agtgcactta gcatacctgt accaccatca tttgttgcac
     1561 tgccaccaat ccctaaaata atggtcttag caccatgatt taatgcatct ttaattagtt
     1621 caccggtacc atatgatgat gtgtataaag gattacgttc ctctttttct aataaatcca
     1681 aacctgacgc tgccgccatt tcaataattg caatttgttg ttcgtctgcg cgtgcataac
     1741 atgcttcaat aggtcgcatt aaagggtcat taacgatgac tgtatactta gtggcacctg
     1801 ttgcatgcat taaagcatct gtggtacctt caccaccatc agccatcgga atgatatcat
     1861 aatgaagggt attcccataa acattagtaa aagcctgttt tataatattg ccaacttgct
     1921 gtgcggtcat actttcctta aatgagtcag gagcgatgac aattttttgt aattgcatag
     1981 tagacatcct ctcattactg ttgtcgctct tattgtatat aaaattacta gtgaactaaa
     2041 gtaatctcta tagtgctaat gagtcacgcg ataaatcagt tacattataa tggcgtataa
     2101 ggatgagggg aaagagacta aatttgctgt gaaagggtat aaagattgaa ataagcatat
     2161 ttgaagtgaa gcggattggc tcgcatattc gagtatgttc gagaacgtag ttatgcatat
     2221 attttgatgt tgaggagtta cacatatgaa actaacacaa acgcatgctg aaattttaaa
     2281 atttatcatt gttggcggca ttaatacgtt aaattattat gttgtctatt tattgctgtt
     2341 aaaattatta cacattgaat atatgattag tcatattacc ggatttctag tcgcttttgt
     2401 gatttcatat tatttgaatt gttattttgt ttacagggta aaacctactt ggagaaaatt
     2461 cattagtttt ccaattacgc agattgtcaa cgtaagctta caaacagttt tattatatgt
     2521 atttgtatcc tggctaaatt tgccagcaga aattgcacca tttgctggtt taattattac
     2581 gatacccatc acatttatat tatctaaatg gattttaaaa gatagcaatt aaagtagcgt
     2641 aaaccactaa aaaaaacttt acttaagcct taatattgcg tgaaatataa taaataaatg
     2701 atataatatg atggtcgcct aacatctcgg gcgttacttt aacgcagaga ggaggtgtat
     2761 gacggatgtt caatttatta attaacatca tgacttcagc tataagcggc tgtcttgttg
     2821 cgttttttgc acattggtta cgaacgcgca acaataaaaa aggtgacaaa taaccaacac
     2881 ctaaataaat cccctcacta tggcagtagt gaggggattt ggtgtattta gatgttcaat
     2941 ttattatgta attaatt
//
LOCUS       DAKTIO010000233         2931 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.240, whole genome shotgun sequence.
ACCESSION   DAKTIO010000233 DAKTIO010000000
VERSION     DAKTIO010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.240"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            181..1116
                     /gene="lukE"
                     /locus_tag="O0293_002591"
     CDS             181..1116
                     /gene="lukE"
                     /locus_tag="O0293_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_114304764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit E"
                     /protein_id="HCY0281445.1"
                     /translation="MFKKKMLAATLSVGLIAPLASPIQESRANTNIENIGDGAEVIKR
                     TEDVSSKKWGVTQNVQFDFVKDKKYNKDALIVKMQGFINSRTSFSDVKGSGYELTKRM
                     IWPFQYNIGLTTKDPNVSLINYLPKNKIETTDVGQTLGYNIGGNFQSAPSIGGNGSFN
                     YSKTISYTQKSYVSEVDKQNSKSVKWGVKANEFVTPDGKKSAHDRYLFVQSPNGPTGS
                     AREYFAPDNQLPPLVQSGFNPSFITTLSHEKGSSDTSEFEISYGRNLDITYATLFPRT
                     GIYAERKHNAFVNRNFVVRYEVNWKTHEIKVKGHN"
     gene            1118..2101
                     /gene="lukD"
                     /locus_tag="O0293_002592"
     CDS             1118..2101
                     /gene="lukD"
                     /locus_tag="O0293_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit D"
                     /protein_id="HCY0281446.1"
                     /translation="MKMKKLVKSSVASSIALLLLSNTVDAAQHITPVSEKKVDDKITL
                     YKTTATSDNDKLNISQILTFNFIKDKSYDKDTLVLKAAGNINSGYKKPNPKDYNYSQF
                     YWGGKYNVSVSSESNDAVNVVDYAPKNQNEEFQVQQTLGYSYGGDINISNGLSGGLNG
                     SKSFSETINYKQESYRTTIDRKTNHKSIGWGVEAHKIMNNGWGPYGRDSYDPTYGNEL
                     FLGGRQSSSNAGQNFLPTHQMPLLARGNFNPEFISVLSHKQNDTKKSKIKVTYQREMD
                     RYTNQWNRLHWVGNNYKNQNTVTFTSTYEVDWQNHTVKLIGTDSKETNPGV"
     gene            2343..2486
                     /locus_tag="O0293_002593"
     CDS             2343..2486
                     /locus_tag="O0293_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="HCY0281447.1"
                     /translation="MEKVLDLDVQVKGNNNTNDSAGDERITSHLFCSFGCEKTGSFNS
                     FCC"
BASE COUNT         1122 a          397 c          487 g          925 t
ORIGIN      
        1 tttaaaggta atgaaggttg cgccaaaatg accggttttc agcagcataa atgaataaat
       61 tgtgtcattt ttaatatttt atagcatttt attttttaat gaacagttaa ttttataaat
      121 aagtgaaata atctagaata aatttgtatt aaaaatttat atagaaagaa agtgaaactt
      181 atgtttaaga aaaaaatgtt agctgcaact ttgtcagtag gactgattgc acctttagca
      241 tctccgattc aagaatctag agcaaatact aatattgaaa atattggtga tggtgctgaa
      301 gtaatcaaac gtacggagga tgtaagtagt aagaaatggg gcgttactca aaatgtccaa
      361 ttcgactttg taaaagataa aaaatataac aaagacgctt taattgttaa aatgcaaggt
      421 tttattaatt ccagaacttc attttcagat gtgaagggta gtggatatga attaactaaa
      481 cgaatgattt ggccattcca atataatata ggactgacga ctaaagatcc aaatgttagc
      541 ttaatcaatt accttcctaa aaacaaaata gaaactactg atgttggtca aacattagga
      601 tataacattg gaggtaattt ccagtcagca ccatctatag gtggcaatgg ctcatttaat
      661 tattctaaaa caattagtta tacccaaaag agttatgtca gtgaagtaga caagcaaaac
      721 tcaaaatctg ttaaatgggg tgttaaagca aacgaatttg ttacgcctga tggaaaaaaa
      781 tctgcgcatg atagatattt attcgtacaa agtccaaatg gtccaacagg ttcagcaaga
      841 gaatattttg ctcctgataa tcaattgcca cctttagttc aaagtggctt taatccatcg
      901 tttatcacta cactatcaca tgaaaaaggt tcaagtgata cgagtgaatt tgaaatttca
      961 tatggtagaa acttagatat tacatatgcg actttattcc ctagaactgg tatttacgca
     1021 gaaagaaagc ataatgcatt tgtaaataga aactttgtag ttagatatga agttaattgg
     1081 aaaacacacg aaattaaagt gaaaggacat aattaatatg aaaatgaaaa aattagtcaa
     1141 atcatcagtt gcttcatcaa ttgcactgct tttgctatcg aatacagttg atgcagctca
     1201 acatatcaca cctgtaagcg agaaaaaagt agatgacaaa atcactttat acaaaacaac
     1261 agcaacatct gataatgata aattgaatat ttctcaaatt ttaacgttta atttcattaa
     1321 ggataaaagt tatgacaaag atacgttagt acttaaggca gccggaaaca ttaattcagg
     1381 ttataaaaag cctaatccaa aagattacaa ttactcacag ttttattggg gcggtaagta
     1441 taatgtttcg gttagttcag aatcaaatga tgctgtaaat gttgttgact atgcacctaa
     1501 aaatcaaaat gaagaattcc aagttcaaca aacattaggt tattcttatg gcggagatat
     1561 taatatatct aatggcttat caggtggatt aaatggatca aaatcatttt cagaaacgat
     1621 aaattataaa caagaaagtt acagaactac gattgataga aaaacaaatc ataaatcaat
     1681 tggctggggt gttgaggcgc acaaaattat gaataatggt tggggaccat atggtagaga
     1741 tagttatgac ccaacatatg gtaatgaact gtttttaggc ggtagacaaa gtagttcaaa
     1801 tgctggtcaa aatttcttgc caacacatca aatgccttta ttggcgcgtg gtaactttaa
     1861 cccagaattt ataagcgtac tttctcataa acaaaatgat acaaaaaaat ctaaaatcaa
     1921 agtaacttac caaagagaaa tggatagata tactaatcaa tggaatcgac tacactgggt
     1981 tggtaataac tacaaaaatc aaaatacagt aacgtttaca tctacttatg aagttgactg
     2041 gcaaaaccat actgttaaat taatcggtac ggattctaaa gaaactaatc ctggagtata
     2101 actgttagaa atagaggaag ttaaacccga aatttaagag ttaaacaaaa aatattcaca
     2161 acatattaaa aaaagaagtt acattatttg tttaaactaa caatagtacc ataaaattaa
     2221 tgacgattta caaattaata acaaataatt attattaaat tcattgattt attgtgtggt
     2281 aatgattaat atgacaattg aagtgggtct taacttcaac ttttaaatag ggggttttat
     2341 ctttggaaaa agttcttgat ttagacgtgc aagttaaagg aaataacaac actaatgatt
     2401 cagcgggtga cgaaagaata actagccatc ttttttgtag ctttggttgt gaaaagacgg
     2461 gtagttttaa cagcttctgt tgttaaggat acagcaaata gagcctgaaa gggctctatt
     2521 tgtcttttta aatatattaa gtgctaaaaa acggaaacag tttattaatc gccaggtatt
     2581 tttataatta gccttctata ttatcagttg agtgtttcaa taaattctga ctttggtgat
     2641 ggaaagttat acttttagtt gtaatatagt tttcatgact tcctgttaaa taagttagga
     2701 ttatgtaatt agcctctatc tttacatata agtaatgaca agctttggtt attatattga
     2761 ccctcttaga acatgtcatt taagaaaata atcaagtcat tgaaattata tgtttatatc
     2821 ctcatcttta aaataacttc aattattatt tgctaaattt tgtataaata gaacaaaact
     2881 gcataaatat ctattaatta atctcaagca gccatctcca aattcgtaat a
//
LOCUS       DAKTIO010000234         2922 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.241, whole genome shotgun sequence.
ACCESSION   DAKTIO010000234 DAKTIO010000000
VERSION     DAKTIO010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.241"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            30..542
                     /locus_tag="O0293_002594"
     CDS             30..542
                     /locus_tag="O0293_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281448.1"
                     /translation="MNSPKTIADNKYSEIQDDKFQLQPGDIIVTKGPVMWGFFGHCSI
                     AIDDKTILQIEGPGDKPTTQSFESFKYNYASGKNDWMKVYRCSYPGAGKKAAEWVKKN
                     YENTNHRYLVTLNLNSKKFTYCTKIIYQAYKFGVSEKSVKSYGLHIISPYAIKDNFID
                     PYKLRLVKAY"
     gene            630..914
                     /locus_tag="O0293_002595"
     CDS             630..914
                     /locus_tag="O0293_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281449.1"
                     /translation="MNELTKEQKYTIAKFYKLYIERSNKGETETVANFFGDAKDAREN
                     YFCDRDYQDFLTNCQILIQNKYLTGEVLDDNIYNISILNKTFIEFEQNFG"
     gene            976..1194
                     /locus_tag="O0293_002596"
     CDS             976..1194
                     /locus_tag="O0293_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281450.1"
                     /translation="MSEWHINNQSTKPFLIQQAEKQLSIVKPLPNGSFQILTEINLEN
                     GNISNIDHSLLVSVDPKALEISIFDAVN"
     gene            complement(1690..1890)
                     /locus_tag="O0293_002597"
     CDS             complement(1690..1890)
                     /locus_tag="O0293_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCY0281451.1"
                     /translation="MNNGTVKWFNAEKGFGFIEREDGSDVFVHFSAIAEDGYKSLEEG
                     QKVEFDIVEGDRGEQAANVVKM"
     gene            complement(2247..2900)
                     /locus_tag="O0293_002598"
     CDS             complement(2247..2900)
                     /locus_tag="O0293_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCY0281452.1"
                     /translation="MAIVSILLIGMAISNVSKGQYAKRFFYFATSCLVLTLVVVSSLS
                     SSANASQTDNGVNRSGSEDPTVYSATSTKKLHKEPATLIKAIDGDTVKLMYKGQPMTF
                     RLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQRTDKYGRGLAYI
                     YADGKMVNEALVRQGLAKVAYVYKPNNTHEQLLRKSEAQAKKEKLNIWSEDNADSGQ"
BASE COUNT         1007 a          480 c          398 g         1037 t
ORIGIN      
        1 tacttatatt attcgttgtc gtatccccaa taaactcacc aaaaacaatt gcagataata
       61 agtattcaga aattcaagat gacaaattcc aattacaacc tggtgatatt attgttacaa
      121 aaggccctgt catgtgggga ttttttggtc attgtagtat cgcgattgat gataaaacga
      181 ttttacaaat tgaggggcct ggcgacaaac caactacaca atccttcgaa tcttttaaat
      241 ataattatgc aagtggcaaa aatgattgga tgaaagttta tcgttgcagc tatcctggtg
      301 caggtaaaaa agcggctgaa tgggttaaga aaaactatga gaatacaaat catcgttatc
      361 tagttacatt aaatttgaac agcaaaaaat ttacgtattg tactaaaatt atctaccaag
      421 cttataaatt tggtgtaagt gaaaaatcag ttaaaagtta tggactacat attatttcac
      481 cctatgcaat taaagataat tttatagatc catacaaact aaggcttgtt aaagcttatt
      541 aacacagttt catcaaatag ttaaattagt ttgtgttttg caataaattg ggtatagatt
      601 acaaatgata tttcaggagg cccaatattt tgaatgaact aactaaagaa caaaagtata
      661 caatagctaa attttacaaa ttatatattg aacgttcgaa taaaggagaa acagaaacag
      721 tcgctaactt ttttggcgat gctaaagatg cacgtgaaaa ctatttttgc gatcgtgatt
      781 atcaagactt cttaactaac tgccaaattt taattcaaaa caaatactta actggtgaag
      841 ttttggacga taacatttac aatatttcta ttttaaacaa gacatttatt gaattcgaac
      901 aaaattttgg ttgaacatga atgtctcaac tataaacttt ctaaccctat tttaaagcag
      961 gagtgaaaaa catacatgag tgaatggcat attaataatc aatctacaaa acctttttta
     1021 atacaacaag cggaaaagca attgtctatt gttaaaccgt tgcctaatgg tagcttccaa
     1081 atactcacag aaataaattt ggagaatggc aatattagta acatcgatca ttctttactt
     1141 gtttcagtag accccaaagc attagaaata agtatatttg acgctgtaaa ttaaacgcaa
     1201 tatttttatt taattatttt aatgtttcgc cttttaaata aaaacaagat tactgaatta
     1261 ttatgagatt ttaaaaacaa taaatattct caataattaa tcagtcttaa taaaaaatta
     1321 acaaaaagtt gattagttaa attattaatt aagtagtaag atattatgcg atgtggtatc
     1381 ctattattgt taaacatttt gagtgtttaa cattacttct tgttatcgcc atatacagac
     1441 tttaattctc gtcattatta aagttctgta ttggcgcttt tttatattca aaatcaaaag
     1501 tcggacagat gaaaagtaaa aaacttttcc atcagttcga tttattttag aaccattata
     1561 tattgttaaa tctccaacta aatttattgg acaaaaaaat aacggctagt gcttttacca
     1621 caccaactgt tatcgattta aagcataaaa aaacagccct atagtaagga ctgtttgaat
     1681 aagttaaaat tacattttaa ctacgtttgc agcttgctcg ccacggtcgc cttcaacgat
     1741 gtcgaattca actttttggc cttcttctaa tgatttgtat ccatcttcag cgattgctga
     1801 gaagtgtacg aatacgtcgc taccatcttc tctttcgatg aaaccaaaac ctttttctgc
     1861 attaaaccat ttaactgtac cgttattcat attgaatacc tccgtcgtgc ttttgcacta
     1921 aatattttgt aacaaattca taaatgaaaa ggagaatatt ctatataaat aactcacatt
     1981 tttcttcacg ctctttatta cttaactttc attatacact tttaaatata gaagtaaagc
     2041 attattttat tttctgtgat aatacacatt gaactatata gtaacattcc attttaatac
     2101 taaatttaaa atttttattt tctaatatta aatacactta cttttgatac tatttacttt
     2161 tttaattctg aattgataat aaatcatatc gtgatctaaa aattataaaa gtgccactag
     2221 cagcagtgac acttttacaa tgagcattat tgacctgaat cagcgttgtc ttcgctccaa
     2281 atatttaatt tctctttttt tgcttgtgct tcactttttc ttaaaagttg ttcatgtgta
     2341 ttgttaggtt tataaacata agcaacttta gccaagcctt gacgaactaa agcttcgttt
     2401 accatttttc catcagcata aatatacgct aagccacgtc catatttatc agttctttga
     2461 cctttgtcaa actcgacttc aattttcttt gcattttcta ccattttttt cgtaaatgca
     2521 cttgcttcag gaccatattt ctctacacct tttttaggat gctttgtttc aggtgtatca
     2581 accaataata gtctgaatgt cattggttga cctttgtaca ttaatttaac cgtatcacca
     2641 tcaatcgctt taattaatgt cgcaggttct ttatgtaatt ttttagttga agttgcacta
     2701 tatactgttg gatcttcaga accacttcta tttacgccat tatctgtttg tgatgcattt
     2761 gctgagctac ttagacttga aactacaact aaagttaaca ctaaacaact agtagcgaaa
     2821 tagaaaaacc tctttgcgta ttgccctttc gaaacattac tgatagccat ccctataagt
     2881 aatattgaaa cgattgccat acatatgcca gcacttaata ag
//
LOCUS       DAKTIO010000235         2830 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.242, whole genome shotgun sequence.
ACCESSION   DAKTIO010000235 DAKTIO010000000
VERSION     DAKTIO010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.242"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..794)
                     /locus_tag="O0293_002599"
     CDS             complement(<1..794)
                     /locus_tag="O0293_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCY0281453.1"
                     /translation="MLNKVWFRTGIALIMLFILIKLFMEVHEVFTPIATIIGSVFLPF
                     LISGFLFYICLPFQNLLEKVGFPRWASITTIMLALFAIIGLIVAFVAPIIISNINNLI
                     SQTPALQKEAEQIIKFALAQMDKLPEDVTSRITNMVKSMGDGVTNILSNSLQYITSLI
                     STIFLLIMVPFFLIYMLKDHEKFIPAVAKFFKGERKVFFVDLLTDLNFTLKSYIQGQV
                     TVSVILGIFLYIGYSIIDLPYIPLLVLFAGVANLIPFLGSWLSFAPA"
     gene            1136..2704
                     /locus_tag="O0293_002600"
     CDS             1136..2704
                     /locus_tag="O0293_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCY0281454.1"
                     /translation="MIEKLVTFLNEVVWSKPLVYGLLITGVLFTLRMRFFQVRHFKEM
                     IRLMFQGEKSPNGISSFQAIAMSLAGRVGTGNIVGVSTAIFIGGPGAVFWMWITAFLG
                     ASSAFIESTLGQIFKRVENNEYRGGPAYYIEYGIGGKFGKIYGIIFAIVTIISVGLLL
                     PGVQSNAIASSMHNAIHVPQWLMGGIVVVILGLIIFGGVRSIANVATAVVPFMAIIYI
                     LMAVIIICINIQEVPALFALIFKSAFGLQSAFGGIVGAMIEIGVKRGLYSNEAGQGTG
                     PHAAAAAEVSHPSKQGLVQAFSVYIDTLFVCTATALIILISGTYNVTDGTVNANGTPH
                     LIKDGGIYVENATGKDYSGTAMYAQAGIDKAFHGSGYQFDPTFSGVGSYFIAFALFFF
                     AFTTILSYYYITETNVAYLTRNQNNQVSSIFINIARVIILFATFYGAVKTADVAWAFG
                     DLGVGLMAWLNIIAIWILHKPAVNALKDYEIQKKRLGNGYNAVYQPDPNKLPNAVFWL
                     KTYPERLKQARAKK"
BASE COUNT          925 a          392 c          526 g          987 t
ORIGIN      
        1 gcaggtgcaa atgataacca tgaacctaag aaaggaatta agtttgcgac accagcaaat
       61 agtactaata aaggaatata tggtaagtca ataattgaat aaccgatata taagaatata
      121 cctaaaataa cactgacagt tacttgacct tgaatgtaag attttaatgt aaagtttaaa
      181 tcagttaata aatctacgaa aaatacttta cgttcacctt tgaaaaattt agcaacagct
      241 gggataaatt tttcatggtc ttttaacata taaattaaga agaatggaac cataatcaat
      301 aagaagatgg ttgaaattaa tgatgtaatg tactgtaatg aattagataa aatattagta
      361 acgccatcac ccattgattt aaccatattt gtaattctac ttgttacatc ttcaggtaat
      421 ttatccattt gagctagtgc gaatttaata atttgctctg cttctttttg tagggcaggt
      481 gtctgactaa ttaaattgtt gatatttgaa atgatgattg gtgcaacaaa cgcaacaatt
      541 aagccgataa tagcaaacaa agctaacatg attgttgtta tactagccca tcttggaaaa
      601 ccgacttttt caagtaagtt ttgaaaaggt agacagatat aaaataaaaa accactaatt
      661 aaaaatggaa gaaatacaga accgatgatt gtagctattg gagtaaatac ttcgtgcact
      721 tccataaata gtttgatgag aatgaacagc ataatcagag cgatgccagt tcggaaccaa
      781 accttgttta acataaacga tgttcctcct atttttctaa acttagttat tatttagtat
      841 atcctaaata gcagtgctta aaaaggttaa tattttttaa atatacttaa attaaccatt
      901 cactttctat tttaagatat ttactttcaa tagtttgtga gacttttcaa tgactcgtaa
      961 gtctgcaatt ggatcaaaca gcaccagttt atatgaataa cttattttaa tattaaacgt
     1021 gtttaaatag tgaaatgatc atattaatat ttataagaat attctgaaaa atatattgaa
     1081 gtgtctcgtg cttaatcgta taatattttt atgtttaaac aataggggga atcttatgat
     1141 tgaaaaatta gtaacctttt taaatgaggt tgtttggagt aagccattag tttatggttt
     1201 gctaattact ggtgtgctat ttacattgcg tatgcgattt tttcaagtta gacattttaa
     1261 agaaatgatt cgattaatgt ttcaaggaga gaagtctcct aatggtattt caagttttca
     1321 agcgatagcc atgtctttag caggcagggt tggtacaggt aatattgtcg gtgtatctac
     1381 tgcaatattt ataggaggac ctggtgcagt attttggatg tggattactg cgtttttagg
     1441 tgcaagtagt gcttttattg aatctacact tggtcaaata ttcaagagag ttgaaaataa
     1501 tgaataccgt ggtggaccag cgtattatat tgaatatggt attggtggta aatttggtaa
     1561 aatttacgga attatctttg ctattgttac gattatctca gtaggtctat tgcttcctgg
     1621 tgtgcaatct aacgctatag caagttctat gcataatgcg attcatgttc cacaatggtt
     1681 aatgggtggt attgttgtag ttattttggg attaattatt tttggtggtg tacgtagtat
     1741 tgccaatgtt gcaacagccg ttgtaccatt tatggcaatt atttacatac tgatggctgt
     1801 cattatcatt tgtatcaata tacaagaagt gccagcgtta tttgcattaa ttttcaaatc
     1861 agcatttgga ttacaatctg cttttggtgg tatcgttggc gcaatgatag agattggtgt
     1921 taaacgtgga ttatattcaa atgaggctgg tcaaggtaca ggtccacacg cagcagcggc
     1981 agcagaagta tcacatccaa gtaaacaagg tctagtacaa gcattttcag tttatattga
     2041 tacattattt gtatgtactg caactgctct gattatactt atttctggta catataatgt
     2101 gactgatggt acggttaatg cgaatggcac accgcattta attaaagatg gcggtattta
     2161 tgttgaaaat gcaacaggta aagattattc aggtactgcg atgtatgcac aagccggcat
     2221 tgataaagcg ttccatggca gtggttatca atttgatcct actttctctg gcgtaggttc
     2281 gtactttatt gcatttgctt tattcttctt tgcatttact acaattttgt cgtactacta
     2341 cattacagaa acaaatgttg cttatttaac gcgtaatcaa aataatcaag tttcatcgat
     2401 atttattaat attgctcgtg tgattatttt gttcgctaca ttttacggtg cagttaaaac
     2461 agctgatgta gcatgggcat tcggtgattt aggtgtaggt ctaatggctt ggttaaatat
     2521 cattgcgatt tggattttac ataagcctgc cgtaaatgct ttaaaagatt atgaaattca
     2581 aaagaaacgt ttaggcaacg gttataatgc agtttatcaa cctgatccga ataaattacc
     2641 taatgctgtc ttttggttga agacgtatcc agaacgttta aaacaagcac gtgccaaaaa
     2701 gtaatctact tttgtttata gtatatgtag tgatcatttg ataaaaaaga aaagtattga
     2761 gaattttagg tgctcagaaa tttgaatttt aaaaatatag tgtctcttgg tacaataaca
     2821 atacaactac
//
LOCUS       DAKTIO010000236         2696 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.243, whole genome shotgun sequence.
ACCESSION   DAKTIO010000236 DAKTIO010000000
VERSION     DAKTIO010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.243"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..338)
                     /gene="deoC"
                     /locus_tag="O0293_002601"
     CDS             complement(<1..338)
                     /gene="deoC"
                     /locus_tag="O0293_002601"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCY0281455.1"
                     /translation="MNSAKLIDHTLLKPESTRTQIDQIIDEAKAYHFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVV"
     gene            653..1363
                     /gene="deoD"
                     /locus_tag="O0293_002602"
     CDS             653..1363
                     /gene="deoD"
                     /locus_tag="O0293_002602"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCY0281456.1"
                     /translation="MTKGTPHIQPNGVKIAKTVLMPGDPLRAKYIADNFLENVEQFND
                     VRNMFGYTGTYKGKEVSVMGSGMGIPSIGIYSYELYNFFDVDTIIRIGSCGALQENVN
                     LYDVIIAQAASTNSNYVDQYNIPGHFAPIADFELVTKAKNVADQIGATTHVGNVLSSD
                     TFYNADPTFNDAWKKMGILGIEMESAGLYLNAIHAGKKALGIFTVSDHILRDEATTPE
                     ERQNSFTQMMEIALEIAE"
     gene            complement(1484..1927)
                     /locus_tag="O0293_002603"
     CDS             complement(1484..1927)
                     /locus_tag="O0293_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HCY0281457.1"
                     /translation="MSNQQDVVKELNQQVANWTVAYTKLHNFHWYVKGPNFFSLHVKF
                     EELYNEASQYVDELAERILAVGGNPVGTLTECLEQSIVKEAAKGYSAEQMVEELSQDF
                     TNISKQLENAIEIAGNAGDDVSEDMFIGMQTSVDKHNWMFKSYLS"
     gene            complement(2127..2540)
                     /locus_tag="O0293_002604"
     CDS             complement(2127..2540)
                     /locus_tag="O0293_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HCY0281458.1"
                     /translation="MPIVYYDGNCIYCYNYVIWLIQHDLPRNYQFATLKGEVGQQFFK
                     QHPEAANKNSVILQKGDRIYFESQAIIKLITALPNRTKLLGVGLWLVPKPVRNFGYRM
                     FANNRNRMWKTTWHQPNDYEKSFFLDDNAKVKLTD"
BASE COUNT          839 a          464 c          401 g          992 t
ORIGIN      
        1 accactgctt caatgtcttg ttgtacatca tcaaaacgtc catcttttaa tgcgccgatg
       61 ttgatgacca tgtcaatttc atctgcacca ttttgaatcg catcttctgt ttcaaatgct
      121 ttcgtcgcag ttgtcgatgc acctaatggg aatcctatta ccgtacaaac taacacctct
      181 gaatcagcta gtcgctctgc tgcatattta acatgcgttg gattcacaca tacagattta
      241 aaatggtatg ctttcgcttc atcgatgatt tgatcgattt gcgtacgtgt tgactcaggc
      301 ttcaataaag tgtgatcaat caattttgca ctattcattt tctatctcct cctttatggt
      361 tgattataaa aatacggttg taaattagtt gattgagcgt caggttcatt taaatatcag
      421 gttagatgtt cgctttttat gtaaccgcat acatatacta ttacattaat tcatttccca
      481 taaacaaaca atacaattga acgtgatatc ttcattatga acgatgactt gacaacaagc
      541 taatcaggat tatattttta taattcttta attctatagt acaaaaattc gcaaaaaagg
      601 gaaacaaatg ttatcttaaa attattaatg aatattaagg agaagataac aaatgacaaa
      661 aggtacacca catattcaac caaatggagt aaaaattgct aaaacagtat taatgcctgg
      721 cgatccgcta cgtgcaaaat atattgctga taatttttta gaaaatgttg aacaatttaa
      781 cgatgtacgt aacatgtttg gttacactgg tacatataaa ggtaaagaag tttctgtaat
      841 gggttctggt atgggtattc caagtattgg tatttactca tatgagttat acaacttctt
      901 tgatgtagat acaatcattc gtatcggttc ttgtggcgca ttacaagaaa atgttaactt
      961 atacgatgtt attattgcac aagctgcatc aactaattca aattatgtag atcaatacaa
     1021 tattccaggt catttcgcgc ctatcgctga cttcgagtta gtaactaaag ctaaaaatgt
     1081 cgctgaccaa atcggtgcta ctacacacgt aggtaacgta ttatcttctg atacatttta
     1141 caatgccgat ccaacattca atgatgcttg gaaaaaaatg ggtattttag gtatcgaaat
     1201 ggaatcagct ggtttatatt taaatgcgat tcatgctggt aaaaaagcac ttggtatttt
     1261 cacagtaagt gatcatattt tacgtgacga agctactaca cctgaagaac gtcaaaattc
     1321 atttacacaa atgatggaaa tcgctttaga aatcgcagag taacttattt aaattgactt
     1381 taattgctct ttaacaatgc gattaaactc aaaaagccaa cacattctgg gcgtatcccc
     1441 atttatgtgt tggcttttat ttatattatt acttatctgt agattagctt aagtaagatt
     1501 taaacatcca attatgttta tctactgatg tttgcatacc tataaacata tcttctgata
     1561 catcatcgcc agcattacca gcaatttcga ttgcgttttc taattgtttt gagatatttg
     1621 tgaagtcttg tgataattct tcaaccattt gttctgcaga gtaacctttc gcagcttctt
     1681 taacaattga ttgctctaag cattcagtta atgtacctac agggtttcct cctaccgcta
     1741 aaattctttc agctaattcg tctacatatt ggcttgcttc attatataat tcttcaaatt
     1801 taacgtgtaa tgagaagaag ttaggccctt tcacatacca gtggaaattg tgtagctttg
     1861 tgtaagctac tgtccagttt gctacttgtt gattcaattc ttttacaaca tcttgttgat
     1921 tactcatatt taatacactc cttaaaattg tctacgtctt gcttgtttat attaattatt
     1981 atattataat aattctaatc tgtaaagtgt ttttgctctc ttttttataa ttattctaat
     2041 ctaatcgtaa agtgttccgc tatttaaatg cctaaatttt aattcatcat ctaatatatc
     2101 cattatcaat tagcaagtat gttttatcaa tcagtaagtt ttactttcgc attatcatct
     2161 aaaaagaacg atttttcata atcatttggt tggtgccatg ttgttttcca cattctattt
     2221 ctattattag caaacattcg ataaccgaaa ttacggactg gtttaggtac aagccataaa
     2281 cctacgccca ataatttagt tcgattaggt agtgctgtga ttagtttaat aatcgcctgc
     2341 gattcaaaat atatacgatc acctttctgt aaaatcacac tatttttatt tgccgcttct
     2401 ggatgttgtt taaaaaattg ttggccaact tcacctttta acgtggcaaa ttgataattt
     2461 cttggtaggt catgttgaat taaccaaatg acatagttat aacaatatat acagtttcca
     2521 tcataatata cgattggcat aatttattcg ctcctttaat agtacttata gtcatttttc
     2581 ctaatttaaa aacatttaaa caaggttgtc acttgataag tcctcgcgac acgaaattgc
     2641 cataaaattt atttttcagt tttatattta ttgtataatg tattttttat ttactt
//
LOCUS       DAKTIO010000237         2695 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.244, whole genome shotgun sequence.
ACCESSION   DAKTIO010000237 DAKTIO010000000
VERSION     DAKTIO010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.244"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(16..924)
                     /locus_tag="O0293_002605"
     CDS             complement(16..924)
                     /locus_tag="O0293_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0281459.1"
                     /translation="MIKLIQISNINKSFNKRCVLKNISFDIEQGKCIALIGKNGAGKS
                     TLIDILIGNVNANSGEIFDKDKLLQSENRSIMFQKTMFPDQLKVIEIINLYQSFYENP
                     LPLEEIIELTKFDSSQLNQFVNKLSGGQQRLLDFVLSLIGQPQLILLDEPTSTMDIEI
                     REYFWSIIENLKEDNRTILYTSHYIEEVERMSDKIILIENGEIILNDSTSHIRTNQQS
                     QITLSDEYIRKLKLDKDDLVIQKNHNGTIKIITSNVNDTILYLQQLHINLDDIEIQKV
                     SIVDSYFNNKKQRGSNYDTKLLENRI"
     gene            complement(1099..2580)
                     /gene="cls"
                     /locus_tag="O0293_002606"
     CDS             complement(1099..2580)
                     /gene="cls"
                     /locus_tag="O0293_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCY0281460.1"
                     /translation="MRFTFSNDLGTLFTIILAIGFIINLVLAFIIIFLERNRRTASST
                     WAWLFVLFVLPLIGFILYLFFGRTVSARKLNKNNGNVLTDFDGLLKQQIESFDKGNYG
                     TDNKQVQKHHDLVRMLLMDQDGFLTENNKVDHFIDGNDLYDQVLKDIKNAKEYIHLEY
                     YTFALDGLGKRILHALEEKLKQGLEVKILYDDVGSKNVKMANFDHFKSLGGEVEAFFA
                     SKLPLLNFRMNNRNHRKIIVIDGQLGYVGGFNIGDEYLGLGKLGYWRDTHLRIQGDAV
                     DALQLRFILDWNSQAHRPQFEYDVKYFPKKNGPLGNSPIQIAASGPASDWHQIEYGYT
                     KMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSGVDVHLMIPCKPDHPLVYWATFSNA
                     SDLLSSGVKIYTYENGFIHSKMCLIDDEIVSVGTANMDFRSFELNFEVNAFVYDENLA
                     KDLRVAYEHDITKSKQLTKESYANRPLSVKFKESLAKLVSPIL"
BASE COUNT          909 a          440 c          336 g         1010 t
ORIGIN      
        1 ttacgcatta taactttaaa ttcgattttc aagtaactta gtatcataat tagatcccct
       61 ttgcttttta ttgttgaagt atgaatcaac aattgagact ttttgtattt caatatcatc
      121 caaattaata tgaagttgtt gaagatataa aatcgtatca tttacatttg aagtaataat
      181 tttgatagtg ccattatgat ttttttgaat aactaaatca tctttatcta gttttaactt
      241 tcttatatat tcatcggata acgtaatctg agattgctga ttggttctaa tatgtgacgt
      301 tgaatcatta agtattattt ctccattttc aatgagaata attttgtctg acatacgttc
      361 gacttcttca atatagtgcg atgtatagag tatcgttcga ttatcttctt ttaaattttc
      421 aataattgac caaaaatatt ctctaatttc tatatccata gtcgatgttg gttcatctaa
      481 taagatcaat tgtggttgtc cgattaaaga taatacaaaa tcgagtaatc gttgttgacc
      541 accagaaagt ttatttacaa attggttcag ttgactagaa tcaaatttcg tcagttctat
      601 tatttcttcc aatggtaatg gattttcgta aaatgattga tataagttga taatctcaat
      661 aacttttaat tgatctggaa acatcgtttt ttggaacatt atactgcgat tttcactttg
      721 taataacttg tctttatcaa atatctcacc agaattagca ttaacattac caattaatat
      781 atcaattaac gttgactttc cagcaccatt ttttccaatt aaagcgatac atttaccttg
      841 ttcaatatcg aacgaaatat tttttagaac acatctttta ttaaatgact tgttgatatt
      901 agatatttga atcaatttaa tcacctctat ttttttctta atttaatatt agtaaattta
      961 ttagatttaa aatagaacac tttgtcatag atttgaaatg acaaatgtca ttattagatt
     1021 tacataatat atttatcgtt attttaattt tgggcaaaat aaaaagagcc tctataatcg
     1081 agactcctta caaataaatt ataaaattgg cgaaactaat tttgctaacg attctttgaa
     1141 tttaacagac agcggtctat tggcatatga ttctttggtt agttgttttg attttgtaat
     1201 atcatgttca taagccaccc ttaaatcttt agcaagattt tcatcatata caaaggcatt
     1261 tacttcaaaa tttaattcaa aacttctaaa gtccatattt gctgtgccca ctgatacgat
     1321 ttcatcatca attaagcaca ttttagaatg tataaatcca ttttcatacg tataaatttt
     1381 aacaccactt gataataagt cagaggcatt tgaaaatgtc gcccaatata ctaatggatg
     1441 atctggctta catggaatca ttaaatgtac atctacacct gatttagcag caattttaat
     1501 ggcatttata tatgaattat ccggaatgaa atatggtgat tgtaaatata cagatttctt
     1561 tgcactcata atcatttttg tataaccgta ttcaatttga tgccagtcac tagccgggcc
     1621 acttgcagct atttgaattg gtgaattgcc caatggtccg ttctttttag ggaaatactt
     1681 aacatcatat tcaaattgtg gacggtgcgc ttgcgaattc cagtctaaaa taaatcgcaa
     1741 ctgcagtgca tcaaccgcat ccccttgtat acgtaaatgc gtatctctcc aatatcctaa
     1801 ttttcctaat cctagatatt catcaccaat gttaaatcct ccgacataac ctagttgacc
     1861 atcgattacg atgatttttc tatgatttct attattcatt ctgaaattca ataacggtaa
     1921 ttttgaagca aaaaatgctt caacttctcc acctaacgat ttaaaatgat caaaatttgc
     1981 catcttaaca tttttagatc caacatcatc atataatatt tttacttcta gaccttgttt
     2041 caatttttct tctaaagcat gtaaaattct tttacctaaa ccatctaaag cgaaagtata
     2101 gtactctaaa tggatatatt cttttgcatt tttaatatct tttaaaactt gatcatataa
     2161 atcatttcca tcaatgaaat gatcaacttt attattttca gttaaaaaac catcttgatc
     2221 catcaaaagc atacgtacta aatcatgatg tttttgaact tgtttgttat cagtaccata
     2281 attaccttta tcaaagcttt ctatttgttg ttttaaaagt ccatcgaaat ccgttaacac
     2341 gttaccattg tttttattca atttgcgtgc cgaaacggtt ctaccaaaaa acaagtaaag
     2401 aataaaacca atcaatggta agacaaaaag tacaaatagc catgcccaag ttgaactcgc
     2461 tgtacgccta tttctttcta aaaagataat aataaaagcc aatactaaat taatgatgaa
     2521 tccaatggct aaaataatag taaataacgt tcctaaatcg tttgaaaatg taaatcgcat
     2581 agtcttttct cctataaaga aaggcacaaa aaaacatttt gcacctttca cgtcatatta
     2641 tttattcaca gataaagtta aaattgcatt gaattcttct tcattatttg ggaat
//
LOCUS       DAKTIO010000238         2681 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.245, whole genome shotgun sequence.
ACCESSION   DAKTIO010000238 DAKTIO010000000
VERSION     DAKTIO010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.245"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            123..236
                     /locus_tag="O0293_002607"
     CDS             123..236
                     /locus_tag="O0293_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281461.1"
                     /translation="MYFKNSSLNYIPTTFGGEPKETLFYKVSNTLCYVNNS"
     gene            complement(240..323)
                     /locus_tag="O0293_002608"
     tRNA            complement(240..323)
                     /locus_tag="O0293_002608"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Leu,seq:caa)
     gene            complement(362..436)
                     /locus_tag="O0293_002609"
     tRNA            complement(362..436)
                     /locus_tag="O0293_002609"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(401..403),aa:Gly,seq:tcc)
     gene            complement(540..614)
                     /locus_tag="O0293_002610"
     tRNA            complement(540..614)
                     /locus_tag="O0293_002610"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(579..581),aa:Gly,seq:tcc)
     gene            complement(622..695)
                     /locus_tag="O0293_002611"
     tRNA            complement(622..695)
                     /locus_tag="O0293_002611"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(661..663),aa:Cys,seq:gca)
     gene            complement(701..772)
                     /locus_tag="O0293_002612"
     tRNA            complement(701..772)
                     /locus_tag="O0293_002612"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(738..740),aa:Gln,seq:ttg)
     gene            complement(784..856)
                     /locus_tag="O0293_002613"
     tRNA            complement(784..856)
                     /locus_tag="O0293_002613"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(821..823),aa:His,seq:gtg)
     gene            complement(859..932)
                     /locus_tag="O0293_002614"
     tRNA            complement(859..932)
                     /locus_tag="O0293_002614"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(898..900),aa:Trp,seq:cca)
     gene            complement(948..1028)
                     /locus_tag="O0293_002615"
     tRNA            complement(948..1028)
                     /locus_tag="O0293_002615"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(992..994),aa:Tyr,seq:gta)
     gene            complement(1034..1109)
                     /locus_tag="O0293_002616"
     tRNA            complement(1034..1109)
                     /locus_tag="O0293_002616"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1074..1076),aa:Thr,seq:tgt)
     gene            complement(1117..1189)
                     /locus_tag="O0293_002617"
     tRNA            complement(1117..1189)
                     /locus_tag="O0293_002617"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1154..1156),aa:Phe,seq:gaa)
     gene            complement(1208..1283)
                     /locus_tag="O0293_002618"
     tRNA            complement(1208..1283)
                     /locus_tag="O0293_002618"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1248..1250),aa:Asp,seq:gtc)
     gene            complement(1293..1366)
                     /locus_tag="O0293_002619"
     tRNA            complement(1293..1366)
                     /locus_tag="O0293_002619"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1330..1332),aa:Met,seq:cat)
     gene            complement(1381..1473)
                     /locus_tag="O0293_002620"
     tRNA            complement(1381..1473)
                     /locus_tag="O0293_002620"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1435..1437),aa:Ser,seq:tga)
     gene            complement(1512..1587)
                     /locus_tag="O0293_002621"
     tRNA            complement(1512..1587)
                     /locus_tag="O0293_002621"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1552..1554),aa:Asp,seq:gtc)
     gene            complement(1598..1687)
                     /locus_tag="O0293_002622"
     tRNA            complement(1598..1687)
                     /locus_tag="O0293_002622"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1649..1651),aa:Ser,seq:tga)
     gene            complement(1697..1770)
                     /locus_tag="O0293_002623"
     tRNA            complement(1697..1770)
                     /locus_tag="O0293_002623"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1734..1736),aa:Ile,seq:cat)
     gene            complement(1799..1872)
                     /locus_tag="O0293_002624"
     tRNA            complement(1799..1872)
                     /locus_tag="O0293_002624"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1836..1838),aa:Met,seq:cat)
     gene            complement(1894..1969)
                     /locus_tag="O0293_002625"
     tRNA            complement(1894..1969)
                     /locus_tag="O0293_002625"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1934..1936),aa:Ala,seq:tgc)
     gene            complement(1987..2060)
                     /locus_tag="O0293_002626"
     tRNA            complement(1987..2060)
                     /locus_tag="O0293_002626"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2024..2026),aa:Pro,seq:tgg)
     gene            complement(2070..2143)
                     /locus_tag="O0293_002627"
     tRNA            complement(2070..2143)
                     /locus_tag="O0293_002627"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2107..2109),aa:Arg,seq:acg)
     gene            complement(2162..2250)
                     /locus_tag="O0293_002628"
     tRNA            complement(2162..2250)
                     /locus_tag="O0293_002628"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2214..2216),aa:Leu,seq:taa)
     gene            complement(2261..2335)
                     /locus_tag="O0293_002629"
     tRNA            complement(2261..2335)
                     /locus_tag="O0293_002629"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2301..2303),aa:Gly,seq:gcc)
     gene            complement(2339..2420)
                     /locus_tag="O0293_002630"
     tRNA            complement(2339..2420)
                     /locus_tag="O0293_002630"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2384..2386),aa:Leu,seq:tag)
     gene            complement(2424..2499)
                     /locus_tag="O0293_002631"
     tRNA            complement(2424..2499)
                     /locus_tag="O0293_002631"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2464..2466),aa:Lys,seq:ttt)
     gene            complement(2505..2580)
                     /locus_tag="O0293_002632"
     tRNA            complement(2505..2580)
                     /locus_tag="O0293_002632"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2545..2547),aa:Thr,seq:tgt)
     gene            complement(2597..2672)
                     /locus_tag="O0293_002633"
     tRNA            complement(2597..2672)
                     /locus_tag="O0293_002633"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2637..2639),aa:Val,seq:tac)
BASE COUNT          768 a          678 c          619 g          616 t
ORIGIN      
        1 tacagaaatt tcaacaactt taaagcacgt ataatgatga ttttcagctt gtacaaagga
       61 gaaaaaagaa gacaaccaag cccaataatg gactggccgc ctaataataa aaactctaaa
      121 agttgtattt taaaaatagt tctttaaatt atatacccac cacatttggt ggagaaccaa
      181 aagaaacctt gttttacaag gtttctaata cgttatgtta tgtaaataac agttaattat
      241 accggtggtc ggggtcgaac cgacactcca caagtggaac gggattttga gtcccgcgcg
      301 tctgccaatt ccgccacacc ggcttaatgg taaacaaaaa acttcccttt ggaagcaatt
      361 atggagcgga agataggatt tacacctata cctcgttccg ggaaggaacg tgttctaaaa
      421 gttgaactac tcccgcaaat attaaattat ggagcggaag ataggattta cacctatacc
      481 tcattccagg aaggaatgta ttctaagagt tgaaatactc ccgcattatt attaaattat
      541 ggagcggaag ataggatttg cacctatacc tcgttccggg aaggaacgtg ttctaaaagt
      601 tgaactactc ccgcataaac ctggaggcgg caaccggatt tgaaccggtg ataaaggttt
      661 tgcagacctc tgccttacca cttggctatg ccgccaataa ctgggctagc tggattcgaa
      721 ccaacgagtg acggagtcaa agtccgttgc cttaccgctt ggctatagcc cattaataat
      781 aagggcggct gaaggggatc gaaccctcga atgtcggaac cacaatccga tgtgttaacc
      841 acttcaccac agccgccatg gcaggggcag taggaatcga acccacacca aaggttttgg
      901 agacctctat tctaccgttg aactatgccc ctattaaaaa taataaatgg aggggggcag
      961 attcgaactg ccgaacccga aggagcggat ttacagtccg ccgcgtttag ccacttcgct
     1021 acccctccat aaatggtgcc ggccagagga cttgaacccc caacctactg attacaagtc
     1081 agttgctcta ccaattgagc taggccggct aagaaatggt tcaggacaga gtcgaactgc
     1141 cgacacatgg agcttcaatc cattgctcta ccaactgagc tactgaacca taataaaaat
     1201 gtaatgatgg cggtctcgac gggaatcgaa cccgcgatct cctgcgtgac aggcaggcgt
     1261 gttaaccgct acactacgag acctataaaa tattgcggga ggcggatttg aaccaccgac
     1321 cttcgggtta tgagcccgac gagctaccga actgctccat cccgcgataa taaaaaataa
     1381 tggcggagga agagggattc gaacccccgc ggcccgttaa ggccctgtcg gttttcaaga
     1441 ccgatccctt cagccggact tgggtattcc tccattatta taggtaaatc gctattaatt
     1501 ataaaattaa atggcggtct cgacgggaat cgaacccgcg atctcctgcg tgacaggcag
     1561 gcgtgttaac cgctacacta cgagaccatt agtaaaacgg aggaagaggg attcgaaccc
     1621 ccgcgagccg ttaagcccct gtcggttttc aagaccgatc ccttcagccg gacttgggta
     1681 ttcctccaaa attatatgga ccttgcagga ctcgaacctg cgaccgaacg gttatgagcc
     1741 gttagctcta accaactgag ctaaaggtcc taaatataat tttacaacta ataaatagtg
     1801 gcggtggagg ggatcgaacc cccgacctca cgggtatgaa ccgtacgctc tagccagctg
     1861 agctacaccg ccttatatag tttgtaaata atatggtgga gactagcggg atcgaaccgc
     1921 tgacctcctg cgtgcaaagc aggcgctctc ccagctgagc taagccccca taataattac
     1981 agtatatcgg gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta
     2041 ccaagctgag ctacttcccg tataattaac gcgcccgata ggagtcgaac ccataacctc
     2101 ttgatccgta gtcaaacgct ctatccaatt gagctacggg cgcatatgtt tttattgaaa
     2161 atggtgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
     2221 ctgtgcgtct gccagttccg ccaccccggc actataaaaa tggagcagaa gacgggattc
     2281 gaacccgcga ccccaacctt ggcaaggttg tattctaccg ctgaactact tctgcatatg
     2341 cgggtgaagg gagtcgaacc cccacgccgt aaggcgctag atcctaagtc tagtgcgtct
     2401 gccaattccg ccacacccgc aaatggtgag ccatagagga ttcgaacctc tgaccctctg
     2461 attaaaagtc agatgctcta ccaactgagc taatggctct tccatggtgc cggccagagg
     2521 acttgaaccc ccaacctact gattacaagt cagttgctct accaattgag ctaggccggc
     2581 aatatgtaag aataaatggt ggagaatgac gggttcgaac cgccgaccct ctgcttgtaa
     2641 ggcagatgct ctcccagctg agctaattct ccgatttaaa a
//
LOCUS       DAKTIO010000239         2670 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.246, whole genome shotgun sequence.
ACCESSION   DAKTIO010000239 DAKTIO010000000
VERSION     DAKTIO010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.246"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            186..893
                     /gene="deoD"
                     /locus_tag="O0293_002634"
     CDS             186..893
                     /gene="deoD"
                     /locus_tag="O0293_002634"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCY0281462.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            900..2252
                     /gene="tet(38)"
                     /locus_tag="O0293_002635"
     CDS             900..2252
                     /gene="tet(38)"
                     /locus_tag="O0293_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HCY0281463.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFPLL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVSLNRWTQSEK
                     "
     gene            2333..>2670
                     /gene="deoC"
                     /locus_tag="O0293_002636"
     CDS             2333..>2670
                     /gene="deoC"
                     /locus_tag="O0293_002636"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCY0281464.1"
                     /translation="MKFEKYIDHTLLKPESTRTQIDQIIDEAKAYNFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVV"
BASE COUNT          868 a          394 c          498 g          910 t
ORIGIN      
        1 aaattataaa aattaaatat agataggctt tgaaaattag ttttatacaa ggttagtagc
       61 tgtaactgta aaatgttctt aatattgtca aaatgtaatg cttgaaagcg cttttaaaaa
      121 atattattat atacatggtt agacaaatag acaaatcact atacaaatat tgggaggaat
      181 attttatgaa atcaacacca cacattaaac caatgaatga cgtcgaaatt gcagaaacgg
      241 ttctattgcc aggagatccg ttaagagcta agttcattgc agaaacttat ttggatgatg
      301 tggaacagtt caatacagtg cgaaacatgt ttggttttac cggaacatat aaaggtaaaa
      361 aagtttctgt catgggttca ggtatgggta tgccatctat tggcatttac tcttatgaat
      421 taattcatac atttggttgt aaaaaattaa ttcgcgttgg ctcttgtggc gcgatgcaag
      481 aaaacattga tttatatgat gtgattattg cacaaggtgc ctctactgat tcaaattacg
      541 ttcaacaata tcaattacca ggtcattttg cgccaattgc ttcttatcaa ttattagaaa
      601 aagcagttga aacagcacgt gacaaaggtg tacgtcatca tgtaggtaat gtgttatcaa
      661 gtgatatttt ctataacgcg gatacaacag cgagtgaacg ttggatgcgt atgggtattt
      721 taggtgtaga aatggaatca gctgcattat acatgaatgc aatttacgct ggtgtcgaag
      781 cattaggtgt gttcacagtg agcgatcatt taattcatga aacgtcaaca acacctgagg
      841 aaagggaacg tgcatttaca gatatgattg aaattgcact gtcattggtg tagatgatta
      901 tgaatgttga atattctaaa ataaagaaag cagtacctat tttattattc ttatttgtat
      961 tcagtttggt tatagacaac tcatttaaat tgatttctgt agccattgct gatgacttaa
     1021 acatatctgt aacgacagta agttggcaag cgacattagc cggtttagta attggtattg
     1081 gcgctgtagt atacgcttca ttatctgatg ccattagtat acgcacacta tttatttatg
     1141 gcgtgatatt aatcattatc ggatcaatta ttggttacat tttccaacat caattcccat
     1201 tacttttagt tggacgtatt attcaaactg ccggtttagc tgctgcagag acattatatg
     1261 tgatatatgt tgcaaagtat ctttctaaag aggaccagaa gacttacctt ggcttaagta
     1321 cgagcagtta ttccttgtca ttagttatcg gtacattatc aggtggattt atttctacgt
     1381 atttacactg gacaaatatg tttttaattg cattaatcgt agtatttacg ttgccattcc
     1441 tatttaaatt attaccaaaa gaaaataata cgaataaagc tcatttagat tttgttggct
     1501 taattctagt ggcaactatt gctacaacag tcatgctgtt tattacgaac tttaattggt
     1561 tatatatgat tggtgcctta attgcgatta tcgtttttgc gctatatatt aaaaatgcgc
     1621 aacgtccatt agtaaataaa tcatttttcc aaaataaacg ttatgcttca tttttattta
     1681 tagtatttgt aatgtatgct atccaattgg gttatatttt tacgttccca ttcataatgg
     1741 agcaaattta tcatctgcaa ctagacacaa catcactgtt attagtaccg ggttatatag
     1801 tagcagtcat tgttggtgca ctaagtggta aaatcggcga atatctgaat tcaaaacaag
     1861 cgattatcac agcaattatt ttaatagcac tgagcttgat tttacctgca tttgcagtag
     1921 gtaatcacat ttcaatcttc gtcatttcta tgatattctt tgcaggtagc tttgctttaa
     1981 tgtatgcacc tttacttaac gaagccatta aaacaataga tcttaatatg acaggtgtgg
     2041 ctattggttt ttataattta attattaatg tggcggtatc tgtaggtatt gcgattgctg
     2101 cggctctaat cgattttaaa gcattaaatt tcccaggcaa tgatgcatta agttcacatt
     2161 tcggtattat tttaattatt ttaggtttaa tgagtattgt cggattagtt ttattcgtca
     2221 gcttaaatcg ttggacacaa tctgaaaaat aaatagatat aaattcgcga gatatattcg
     2281 tatttatagt aaaattaaat aaagagatta tataacacga ggagtagtaa gtatgaaatt
     2341 tgagaaatat atagatcaca ctttattgaa gcctgagtca acacgtacgc aaatcgatca
     2401 aatcatcgat gaagcgaaag catacaattt taaatctgta tgtgtgaatc caacacatgt
     2461 taaatatgca gcagagcgac tagctgattc agaggtgctc gtttgtacgg taataggatt
     2521 cccattaggt gcgtcgacaa ctgcaacgaa agcatttgaa acagaagatg caattcaaaa
     2581 tggtgcagat gaaattgaca tggtcatcaa catcggcgca ttaaaagatg gacgttttga
     2641 tgatgtacaa caagacattg aagcagtggt
//
LOCUS       DAKTIO010000240         2651 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.247, whole genome shotgun sequence.
ACCESSION   DAKTIO010000240 DAKTIO010000000
VERSION     DAKTIO010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.247"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            1..762
                     /locus_tag="O0293_002637"
                     /pseudo
     CDS             1..762
                     /locus_tag="O0293_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501248.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            759..2083
                     /locus_tag="O0293_002638"
                     /pseudo
     CDS             759..2083
                     /locus_tag="O0293_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2083..2409
                     /locus_tag="O0293_002639"
     CDS             2083..2409
                     /locus_tag="O0293_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281465.1"
                     /translation="MFTTYKNINELENAYDEERKQLNDAFNQIDELRHQTRKKCEQMY
                     DHFLYLKHKMNYSEDAMIRMTRIIESFDRETNQRIRHHEMKLEDYKDELRREYLKQSD
                     RIEGDE"
     gene            2412..>2651
                     /locus_tag="O0293_002640"
     CDS             2412..>2651
                     /locus_tag="O0293_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281466.1"
                     /translation="MGLDFSGLPDLAVLEQMKEKEQISEVIAPEHVRMHHDHQNKLKS
                     DEKILLDQMVSHFKKFEDDFKNAAQGAWVKNATDEL"
BASE COUNT         1087 a          360 c          500 g          704 t
ORIGIN      
        1 gctcggtata tgtttggtat tactcattat attgattgga ggttgtgtca ttatgacaaa
       61 aacaaatggt cgaaacgctc aaattaaaga aaatttcaac aaaacattaa gtgtatatcc
      121 aaccaagaat ctcgatgatt tttacgataa ggaaggtttt cgagatcaag aatttgataa
      181 aagagataaa gggacttgga ttatttattc tgaaatggtt atcgaaccaa aagggaaaaa
      241 tatggaatcg agaggaatgg tgctctatat caatcgcaat actagaacaa cgaagggtaa
      301 ttttattgtc accgaaataa ctgaagatag taaaggatat tcacgtagta aagaaaaaaa
      361 tatcctgtca agatggaaaa taatcgaatt attccaacaa agcctatacc ggatgacaag
      421 ttaaaaaaag agattgaaaa ctttaagttc tttgtacaat atggaaattt taaagatttt
      481 aaagattata aaaatggtga tatttcatat aatcctaatg tgccaagtta ttctgcaaag
      541 tatcaattga ataatgatga ctataatgtt caacagttaa gaaaacgata tcatattcca
      601 accaaacaag cgcccgaatt aaaattgaaa ggatccggca atttaaaagg ctcatccgta
      661 ggatctaagg atctagaatt tacgtttgta gaaaatcaag aagagaatat ctatttttca
      721 gattcggtcg aatttacacc tagcgaggat gataaatcat gagtcaaacg gaatatcaaa
      781 taaaatctgg caatataaaa ggtaactctg aagaaacaag tacagtatct aatataagtt
      841 atgaaataga aaacgcaaat aacagtggtt taaaacaaaa taaaattgat aaacaaatta
      901 aaaagttaca agaaaaaaat aaattcccta aaaatctttc atatcttaaa agttatacgg
      961 accccaaaac aggcacgact acaagcgcct ttttaaataa agacactggc aaagttactt
     1021 taggtatgac aggtactaat gtacacaaag acgcaatatt aaaacaaaca tttggtgttc
     1081 cttcttatca aggatatata gatgtgagtg aaacgctaaa ggatattggg gccgatgtca
     1141 atattggcct tcattccgtc acagataaag atccacatta taaaaatacc caagacttta
     1201 tcaaaaatat caaaaaagac tatgatattg atattattac cggacattcg ctgggcggta
     1261 gagatgcgat gattttaggt atgagtaatg atattaaaca tatcgttgtg tataatccag
     1321 ctccattagc gatcaaagat gtgagcggcc tttatgccga tcaagaagaa cttaaaaaat
     1381 tgattgaaaa gtacgatggt cacattgtaa gatttgtgtc tgatgaagac gaattagatg
     1441 caggtgtccg caatcattta tatgaaactg ctggagaaaa aatagtactt aaaaatggag
     1501 aaggccatgc aatgagtggt attttaatga gcagaacaca ggctataatc ttagctgaat
     1561 taaacaaagt taaaggctac caagacgaaa ataataaagc attaaaatcc gttcgtaaac
     1621 aaacgaggca tagattacat aaagtagaga cgttaagagc gaattggatt caaacaacgg
     1681 gtggatcact ctcttcctcc caacaacaat tattagaagc tttaacagca ctaaccattg
     1741 ccgaaggctt aaatcaatta gtgaatgaag aaagccaaca tttgaaaaaa tgtatcacgc
     1801 gatggcacat aaatttggag acaactggaa aaaagcgcaa gaagttggaa atgaaattgg
     1861 tgaaaaatta acctctgaag aggttataga tgaattaaga aaaggtggcg cgtatgaaag
     1921 taaacttgaa acagatccca aaagaaaaat tgatgataag ataaagaaat taaatgatgt
     1981 ttataaaaat tgtaatggct atatcgcaaa aattaaacag agtatcgaag caattgtttc
     2041 taatgaccaa atgttagcga gccagattga tgggatgatg taatgtttac tacgtataaa
     2101 aatattaatg aacttgaaaa tgcctatgat gaagaaagaa aacaattgaa tgatgcattc
     2161 aatcaaattg atgaattaag acatcaaaca cgcaagaaat gtgaacaaat gtatgatcat
     2221 ttcttatatc tcaaacataa aatgaattat agtgaagacg ctatgatcag gatgacacgt
     2281 attatagaat ctttcgatag agaaacgaat caacgtatcc gacatcacga aatgaaatta
     2341 gaagattata aagatgagtt aagaagagaa tatctaaaac aatctgacag aattgaagga
     2401 gatgaataag cgtgggactt gattttagtg gtttaccaga tttagcagta ttggaacaaa
     2461 tgaaggaaaa agaacagatt agtgaggtta ttgcgcctga acatgttcgt atgcatcatg
     2521 atcatcaaaa taagctgaaa agtgatgaga aaatattact tgaccaaatg gtaagtcatt
     2581 tcaaaaaatt tgaagatgat tttaaaaatg cggcacaagg ggcttgggtg aaaaatgcca
     2641 cagacgaatt a
//
LOCUS       DAKTIO010000241         2629 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.248, whole genome shotgun sequence.
ACCESSION   DAKTIO010000241 DAKTIO010000000
VERSION     DAKTIO010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.248"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(136..705)
                     /locus_tag="O0293_002641"
     CDS             complement(136..705)
                     /locus_tag="O0293_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HCY0281467.1"
                     /translation="MYSQNIYVIRKGDMVIRPAFDDDDQRNGSEIIRFDKTRIQNPFK
                     VQKIIERSCKFYGNTYLGKKAETNRITGISSKPPILLTPLFPTYFFPTHSDRQNENIW
                     LNMHYIESIKELKNRKCKVTFINNESIILHVSYHSLWHQYNNSIFYYYMVDKQSRMIS
                     KNPDQPIDYNKATLNVFEALTRYSLFEDK"
     gene            915..1133
                     /locus_tag="O0293_002642"
     CDS             915..1133
                     /locus_tag="O0293_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HCY0281468.1"
                     /translation="MKYNTNVKHTTLEAFVTTVNDLGIELIINEALREVRKRQLIELI
                     DDALVNKDEAAFNQYTAEYKNLEAFLGE"
     gene            complement(1214..2200)
                     /locus_tag="O0293_002643"
     CDS             complement(1214..2200)
                     /locus_tag="O0293_002643"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCY0281469.1"
                     /translation="MKFISNNNITDPTLNLAMEEYVLKNLPAEESYFLFYINRPSIIV
                     GKNQNTIEEVNQTYIDAHNIDVVRRISGGGAVYHDTGNLNFSFITDDDGNSFHNFQKF
                     TEPIVQALQSLGVNAELTGRNDIQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQ
                     NALKVNPAKIKSKGIKSVRKRVANIQEFLNDPLEIEEFKKIILKTIFGETEVEEYKLT
                     DEDWENIEKLSNDKYRTWEWNYGRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIF
                     GDFFGVGDVTDLENALVGCLHDFEHIEEALSEYDLYHYFGDIDRHELIRLMS"
     gene            2399..2575
                     /locus_tag="O0293_002644"
     CDS             2399..2575
                     /locus_tag="O0293_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkvS family protein"
                     /protein_id="HCY0281470.1"
                     /translation="MTVAEVGNIVEFMDGLRGRVEKINDNSVIVDLTIMENFNDLDLP
                     EKTVINHKRYKIVE"
BASE COUNT          897 a          389 c          364 g          979 t
ORIGIN      
        1 tgtatgtgtt tgaatatcaa gcacaaatat tattataaca agattaagtt aatttgcaat
       61 agatacctta aaaaaatatg cagaacattt acactatatt tataaaacat tccgcatatt
      121 ttaaaaataa acaatttatt tatcttcaaa taaagaatag cgtgtcaatg cttcaaacac
      181 attcaatgtg gctttattat aatctattgg ttggtcggga ttttttgata tcatgcgaga
      241 ttgtttatct accatgtaat agtaaaaaat ggaattgtta tattgatgcc ataaactgtg
      301 gtatgaaaca tgaagaatga ttgattcatt attaataaat gtcactttac atttacgatt
      361 ttttaattct ttaatacttt cgatataatg catatttaac caaatatttt cattttgtct
      421 gtcagaatgt gttgggaaaa aataagttgg aaataatggt gttagtaaaa taggtggttt
      481 actagaaatg ccagtaatgc ggtttgtctc tgctttcttg ccaagataag tattaccata
      541 aaatttgcaa gagcgttcaa tgattttctg gactttaaaa ggattttgaa tacgcgtttt
      601 gtcaaaccga attatttcac taccgtttct ttgatcgtca tcatcaaatg ctggtcgaat
      661 aaccatgtct cctttgcgta tcacataaat attttgagaa tacataagca aaacctcgct
      721 ttattaagtt ttaaaccatt aatataaaaa tacaattaat gatataaaga tattttataa
      781 taaaattctg agttgtcaag ttattatttt ggtgaaaata tacaataatt tttaactaat
      841 agtcagaata ttcattgatt tcaaaattag aaaaatgtat acttattata aattaaagaa
      901 aggtagtggt ttctatgaaa tacaatacta atgttaaaca tacaacttta gaagcgtttg
      961 tcacaactgt caatgatttg ggtattgaat taattatcaa tgaagcactt cgagaggtaa
     1021 gaaaacgaca gctcatagaa cttatagatg acgcactcgt caataaagat gaagcagcat
     1081 ttaatcaata tacggcagaa tacaaaaatt tggaggcatt tctcggtgaa taacattgat
     1141 tcgaattcca taacgcaatc ataaaaggac tattcttaac atctcataca tgtaagaata
     1201 gtcctttaat cgtttatgac attaatctaa ttaattcatg tctatctatg tcaccgaaat
     1261 aatgatataa atcatattct gatagtgctt cttctatgtg ctcaaagtcg tgtagacagc
     1321 caactaatgc attttcaaga tcagtgacat ctccgacacc aaagaaatca ccgaatattt
     1381 ttgcatgttc gattttacct cgtttaacat caaacttaat ttgtacaaat cctttttcaa
     1441 atttttcctc gcgttcaaag ttatatttag ggtttctacc ataattccat tcccaagttc
     1501 tatatttgtc gttacttaac ttttcaatat tttcccaatc ttcatccgtt aatttatatt
     1561 cttctacttc agtttctcca aagatagttt tcaagatgat ttttttaaat tcttcaattt
     1621 ctaaagggtc atttaaaaat tcttgaatgt ttgctacccg tttacgaaca gatttaatac
     1681 cttttgattt aatcttagct ggattaactt ttagtgcatt ctgaacttcg tctaaatcac
     1741 tatttaacat caacgtacca tgactaaaca ttctattttt aactttaacc atagcattcc
     1801 ctgagatttt cgcttgtcca acttgaatat cgttacgacc agttaattca gcatttacgc
     1861 ctaaagattg taatgcttga acaattggtt cagtgaattt ttggaaattg tggaaactgt
     1921 taccatcatc atctgttata aaactaaagt ttaaattgcc agtatcatga taaacagcgc
     1981 caccaccaga aattcttctt actacatcga tgttgtgagc atcgatatat gtctgattta
     2041 cttcctctat cgtattttga ttctttccaa caatgataga tggtctattt atgtaaaata
     2101 aaaagtaact ttcttctgct ggtaaatttt ttaaaacata ttcttccatt gctaagttta
     2161 aagttggatc tgtaatatta ttattactaa tgaatttcat tacaatctct cccttatatc
     2221 tatatatatt ctctacttat ttatgcctta actttgctca aaatgccaat tatatactca
     2281 caaatcattc taaatatcgg cttaaagtta cattttatta tttttgattg ttaatagttt
     2341 atttgttaag tgaattttag tacaatataa tttagttatg atttaaggag gacttaaaat
     2401 gactgttgca gaagtgggta acattgttga gtttatggat ggattaagag gtcgtgttga
     2461 aaaaatcaac gataactctg ttattgttga cttaacaatt atggaaaatt ttaatgacct
     2521 tgatttaccg gaaaaaactg ttatcaatca taaacgatat aagattgttg aataagaagg
     2581 taagttataa tgaataaaat ctcgaaggct ttaacttggt ttattataa
//
LOCUS       DAKTIO010000242         2616 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.249, whole genome shotgun sequence.
ACCESSION   DAKTIO010000242 DAKTIO010000000
VERSION     DAKTIO010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.249"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(78..269)
                     /locus_tag="O0293_002645"
     CDS             complement(78..269)
                     /locus_tag="O0293_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0281471.1"
                     /translation="MPEQLSVRKWRLVRDLKQQEVADILGVNAKTVGHWEKDDTNLSN
                     VTVYALAKLYDIEVDQIKV"
     gene            430..759
                     /locus_tag="O0293_002646"
     CDS             430..759
                     /locus_tag="O0293_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0281472.1"
                     /translation="MENNKVRKILSENLQELMNDKNIDQRELAEAIGVSQPTVSNWIQ
                     QTKYPRIKRIQQLADYFNVPKSRITESKKDIHQETIAAHFDKEGLTEEEIEEVNRFIE
                     WVRNRDK"
     gene            772..1233
                     /locus_tag="O0293_002647"
     CDS             772..1233
                     /locus_tag="O0293_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCY0281473.1"
                     /translation="MGLYEELCINNEKIKIEETDQLPNFQPGCYMNGKIYIRRNLSEV
                     RKAEVLYEQLAHHKLTYGNILDQTKWINRKFENYARRHGFTSAVPLHEIVEAHNYGVR
                     NLYELSEYLQLSESYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI"
     gene            1251..1685
                     /locus_tag="O0293_002648"
     CDS             1251..1685
                     /locus_tag="O0293_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281474.1"
                     /translation="MKKVIGLLLVSTLALTACGEKEKPKKEENKKSQTQNHKDSKPKT
                     QQEKMKKVEDKNPPNNSIQNNSNNQNQSQNNQLNNNSDPSNNTPANINENDSQNTNLN
                     DEYVVSPGWTKDEQAKAFEEYKKGKEEEARAGASAVPGANIN"
     gene            1714..2109
                     /locus_tag="O0293_002649"
     CDS             1714..2109
                     /locus_tag="O0293_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281475.1"
                     /translation="METNKTIDLMNYVEFPKRYTEAKGKLVAQPITTINSARRVENED
                     MTVCYILDQDDDVMDFIFDRDIITVYCPENGTATDEYFCEIIFNSDDTFTLKRLSNYV
                     TIKDRSYPMSKINDVNITGKVVRLFRDFK"
     gene            2199..2345
                     /locus_tag="O0293_002650"
     CDS             2199..2345
                     /locus_tag="O0293_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281476.1"
                     /translation="MNGGENFMVDKNKKQETTRSNPLNKSFEKSGASEKLKSTLSEKA
                     KKKD"
     gene            complement(2342..>2616)
                     /locus_tag="O0293_002651"
     CDS             complement(2342..>2616)
                     /locus_tag="O0293_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCY0281477.1"
                     /translation="CISLLDKRDKLFNNNGKPVFIVIKDFENRIIEEGELKTYNSAGS
                     DFDLLEVERQDFKVSDLPSNDELYIKHTLVDLKQQIKLDLYLMNEY"
BASE COUNT         1049 a          396 c          419 g          752 t
ORIGIN      
        1 ttcctccttt taagttgttt atgtccattt gttacattac ctaaaagtta taaaaagtta
       61 tacgaaggtc aaaaaattta gacctttatc tggtctacct caatatcata taactttgct
      121 aaagcgtaaa ctgtaacatt acttaaatta gtgtcatcct tttcccaatg accaactgtc
      181 tttgcattga cgcccaatat atctgctact tcttgctgtt tcaagtccct tacaagtctc
      241 cattttctta cggataattg ttccggcatg tcgtccacct ccctttcaca cgtataactt
      301 tatattactt tgtattactt gtcaacagat aaagttatat ttcttcataa aaagttataa
      361 aaagtattgt aaagtaatgt cggttatggt aagttaatat tacattaagt aatattaaag
      421 gagacaacta tggagaataa taaagtcaga aaaattttat ctgaaaacct tcaagaactt
      481 atgaatgata aaaatattga tcagagagaa cttgctgaag ctattggagt ttctcaacct
      541 acagtctcca attggattca acaaactaaa tatccacgaa ttaaaagaat tcaacaactt
      601 gcagattact tcaatgtacc gaaatcaaga attactgaat caaaaaaaga tatacatcaa
      661 gaaacaattg ctgctcattt tgataaggag ggattaactg aagaagagat tgaagaagta
      721 aatagattca ttgaatgggt tagaaataga gacaaataaa gggtgtttat attgggatta
      781 tacgaagaac tttgcataaa taacgaaaaa ataaagatag aagaaactga ccagcttcca
      841 aatttccaac ctggatgcta tatgaacgga aaaatttata taaggcgtaa tttatcagaa
      901 gtacgtaaag cagaagtgtt atatgagcaa cttgcccacc acaagttgac gtatggcaac
      961 attttagacc aaacaaaatg gattaataga aaatttgaaa attatgcacg tagacatggt
     1021 tttacttcag ctgtaccgct acatgaaatt gtagaagctc acaactacgg tgttcgtaat
     1081 ttgtacgagt tgtcagagta tctgcagttg agtgaatcat acatactaga agctatagaa
     1141 caatataaaa agatatatgg tattggaact cactatggcg agtattctat tacgtttgag
     1201 ccgttgagag tttttaaata taaggaaata taaacaaagg agagatacat atgaaaaaag
     1261 taatcggact gctactagta agtacattag ctttaacagc ttgtggtgaa aaagaaaaac
     1321 caaaaaaaga agaaaataaa aagtcacaaa cacaaaatca caaagatagc aaaccaaaaa
     1381 cgcaacaaga aaaaatgaaa aaagttgaag ataaaaatcc acctaataat agcatacaaa
     1441 ataattcaaa caatcaaaac caatcacaaa acaatcaact taataataat tcagatccat
     1501 ctaataatac tcctgcaaat ataaatgaaa acgattcaca aaatactaat ttaaatgatg
     1561 agtatgtcgt ttcgcctggc tggactaaag atgaacaggc taaagctttt gaagagtaca
     1621 aaaaaggaaa agaagaggaa gcaagagctg gtgctagcgc agtaccagga gccaatatta
     1681 actaataaaa caatataaga aagaagagct aatatggaaa caaataaaac aatcgattta
     1741 atgaattatg tggaatttcc aaaaagatac acagaggcaa aaggcaaatt agttgcacaa
     1801 ccaataacta ctataaatag cgcaagaaga gttgaaaatg aagatatgac tgtttgctac
     1861 attttagatc aggatgatga tgtaatggac tttatctttg acagagatat aattactgtt
     1921 tactgtcctg aaaacggaac tgcgactgat gaatattttt gtgaaattat atttaactca
     1981 gatgacacat ttaccctaaa gcgattatct aattacgtta ccattaaaga tagaagctac
     2041 ccaatgtcaa aaataaatga cgtaaacatt acgggcaaag tcgtcagatt atttagagat
     2101 tttaaataaa cttggcttta attacgatta aaagtaccta tatagcgtga cgagaaaaag
     2161 gattaaaaaa aatcaaaaaa cgcctactag tgtagacgtt gaatggtggt gagaatttta
     2221 tggtagataa aaacaaaaaa caagaaacta ctcgtagtaa cccattaaac aaaagttttg
     2281 aaaagtcagg cgccagcgaa aaattaaaaa gcactttatc agaaaaagct aagaaaaaag
     2341 attagtattc attcattaaa tataaatcca atttaatttg ttgtttaagg tctacaagcg
     2401 tatgtttaat atacaattca tcgtttgacg gtaaatcaga tactttgaaa tcttgtcgct
     2461 caacttctag taaatcgaaa tcgctaccag ctgaattata ggttttaagt tcaccctctt
     2521 caatgattct gttttcaaag tcttttataa ctataaatac tggtttaccg ttgttattaa
     2581 acaacttgtc tcttttgtct aataagctta tacaat
//
LOCUS       DAKTIO010000243         2578 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.250, whole genome shotgun sequence.
ACCESSION   DAKTIO010000243 DAKTIO010000000
VERSION     DAKTIO010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.250"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(6..620)
                     /locus_tag="O0293_002652"
     CDS             complement(6..620)
                     /locus_tag="O0293_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCY0281478.1"
                     /translation="MSQWKRISLLIVFTLVFGIIAFFHESRLGKWIDNEVYEFVYSSE
                     SFITTSIMLGATKVGEVWAMLCISLLLVAYLMLKRHKIEALFFALTMALSGILNPALK
                     NIFDRERPTLLRLIDITGFSFPSGHAMGSTAYFGSGIYLLNRLNQGNSKGILIGLCAA
                     MILLISISRVYLGVHYPTDIIAGIIGGLFCIILSTLLLRNKLIN"
     gene            complement(637..1707)
                     /locus_tag="O0293_002653"
     CDS             complement(637..1707)
                     /locus_tag="O0293_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCY0281479.1"
                     /translation="MTKIAFTGGGTVGHVSVNLSLIPTALSQGYEALYIGSKNGIERE
                     MIESQLPEIKYYPISSGKLRRYISLENAKDVFKVLKGILDARKVLKKEKPDLLFSKGG
                     FVSVPVVIAAKSLNIPTLIHESDLTPGLANKIALKFAKKIYTTFEETLNYLPKEKADF
                     IGATIREDLKNGNAHNGYQLTGFNENKKVLLVMGGSLGSKKLNSIIRENLDALLQQYQ
                     VIHLTGKGLKDAQVKKSGYIQYEFVKEDLTDLLAITDTVISRAGSNAIYEFLTLRIPM
                     LLVPLGLDQSRGDQIDNANHFADKGYAKAIDEEQLTAQILLQELNEMEQERTRIINNM
                     KSYEQSYTKEALFDKMIKDALN"
     gene            complement(1719..2228)
                     /locus_tag="O0293_002654"
     CDS             complement(1719..2228)
                     /locus_tag="O0293_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0281480.1"
                     /translation="MIRLGKMSDLDQILNLVEEAKELMKEHDNEQWDDQYPLLEHFEE
                     DIAKDYLYVLEENDKIYGFIVVDQDQAEWYDDIDWPVNREGAFVIHRLTGSKEYKGAA
                     TELFNYVIDVVKARGAEVILTDTFALNKPAQGLFAKFGFHKVGEQLMEYPPYDKGEPF
                     YAYYKNLKE"
BASE COUNT          844 a          461 c          331 g          942 t
ORIGIN      
        1 actatttaat ttattaattt atttctaagt aataacgttg ataaaataat gcaaaataat
       61 ccaccaataa tgccggcaat aatatctgtt ggataatgta cacctagata tacacgtgat
      121 atggaaatca ataaaatcat agctgcacat aaccctataa gaataccttt tgaattacct
      181 tgatttaatc gatttaatag atagatacca cttccaaaat atgcagttga tcccatagca
      241 tgaccgctag gaaaactaaa tcctgttata tcaattaaac gcagcaatgt aggtctttct
      301 ctatcgaata tattttttaa tgctggattc aaaattccag ataatgccat tgttaatgca
      361 aaaaataatg cttcaatttt gtggcgcttt aacatgagat atgccacaag aagtaatgaa
      421 atacataaca ttgcccagac ttcacctact ttagtagccc caagcatgat agatgtcgta
      481 ataaagctct ctgatgaata tacaaactca taaacttcat tatcaatcca tttcccaagt
      541 cttgattcgt ggaaaaacgc gataattcca aaaaccaatg taaaaacgat gagcaaagag
      601 atacgtttcc attgactcat aaagcattac cccccattaa ttcaatgcgt ctttaatcat
      661 cttatcaaat aaagcttctt tcgtataact ttgttcatac gatttcatat tattgataat
      721 tcgagttctt tcctgttcca tttcatttag ttcttgtaat aaaatttgtg ctgttaattg
      781 ttcttcatca atcgctttag catatccttt atcagcaaaa tgatttgcat tgtcaatttg
      841 gtcgcctcgg gattgatcta aacctaatgg tactaataac attggtatac gtaatgttaa
      901 gaactcataa atcgcatttg atccagctct acttattact gtatccgtaa ttgctaataa
      961 atctgttaaa tcctctttaa caaattcata ttgtatatat cctgattttt taacttgagc
     1021 atcttttaat cctttaccag ttaaatgtat cacttgatat tgttgtaata atgcatctaa
     1081 gttttcgcga ataatgctat ttaatttttt acttcctaag cttccaccca taacgagtaa
     1141 aactttttta ttttcattaa agcctgttaa ttgataacca ttatgtgcat taccattttt
     1201 taaatcttct cgaattgttg ctccaataaa atcagctttc tctttaggta agtagtttag
     1261 cgtttcttca aatgttgtat atattttctt ggcaaattta agtgctatct tattcgctaa
     1321 tcctggtgtt aagtcagatt catgaataag agttggtata tttaatgatt tggctgcaat
     1381 aacaacaggc acagatacaa atccaccttt tgaaaataat agatcaggtt tttctttttt
     1441 caaaacttta cgagcatcaa gaataccttt caatacttta aatacgtctt tggcattttc
     1501 taaagaaata tatcttctta atttaccact cgaaatagga taatacttaa tttctggtag
     1561 ttgtgattca atcatttctc tttcaatacc atttttagaa ccaatataaa gcgcttcata
     1621 accttgtgat aatgcagttg gaattaaact taaatttact gatacgtgtc caactgttcc
     1681 ccctccggta aatgcgattt tcgtcattaa tattacctct attcttttaa atttttataa
     1741 tatgcataaa atggttcacc tttatcatat ggcggatatt ccattaattg ttcaccgacc
     1801 ttatgaaatc caaatttggc aaataaacct tgtgcaggtt tgtttaacgc aaaggtgtcc
     1861 gttaaaataa cttctgcacc acgtgcttta actacatcaa taacataatt gaataattct
     1921 gtagcagctc ctttatattc tttcgaacca gttaatcgat gaataacaaa ggcgccttct
     1981 ctatttactg gccagtcaat gtcatcatac cattctgctt ggtcttggtc gacaacaata
     2041 aagccataaa ttttgtcatt ttcctctaat acgtacaaat aatctttagc aatatcttct
     2101 tcaaaatgtt ctaaaagtgg gtactgatcg tcccattgct cgttgtcgtg ttctttcatt
     2161 aattcttttg cttcttctac tagatttaag atttgatcta aatctgacat tttacctaga
     2221 cggatcatgt acgtcactcc ttatatcatt atgttaaaca tgtttttcag tacatgttaa
     2281 tcttacacac tattaacatg ctttaaacct ttattatgaa tgaaaaatag taattatcac
     2341 actatgggat aaaaataacc aataaaagta tcatatacca attaattgat tgttaaaact
     2401 agtgaataat actatttaaa aagtatatgc tcattgcata ttaaaccatg aattgttatc
     2461 agcgatgata gaaattgcgt tatttacatc atttgtaaat gtctaattta tatcttttag
     2521 agttagacgt caataaaaat aaacacgcct cctaaattgc tatctttgca aatttagg
//
LOCUS       DAKTIO010000244         2571 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.251, whole genome shotgun sequence.
ACCESSION   DAKTIO010000244 DAKTIO010000000
VERSION     DAKTIO010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.251"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..584)
                     /locus_tag="O0293_002655"
     CDS             complement(<1..584)
                     /locus_tag="O0293_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0281481.1"
                     /translation="MTDLLKIKNFRLFFLAEIISAFGVGISTVGANWYLIDKTNDSQL
                     LGIMLALNVLSGFLASPIIGGLADKYNRRNIILITYLLQVILYLLIVIALVMIGFETY
                     LVIGFAIVNGIGWTTYMATSRSLVKQILKPDQYTDANSLLEISLQTGMFIAGGLSGIL
                     YKINGFTLIIAMTIMMFLISIFMLFRLHVDKPTHS"
     gene            complement(577..1127)
                     /locus_tag="O0293_002656"
                     /pseudo
     CDS             complement(577..1127)
                     /locus_tag="O0293_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498649.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
     gene            1240..2100
                     /locus_tag="O0293_002657"
     CDS             1240..2100
                     /locus_tag="O0293_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0281482.1"
                     /translation="MNLNILKSFLVTSETKNLTKASELLNYSQSTVSTHIEKLERQLD
                     VKLFYRKKYGMELTEEGLAYVKYAKVILDSNSEYEREIKGLYNKKVNISINMQESQYL
                     YRYYNKISEWLAEHPYVNLKFKSAHSNFYIKEEIANFKSDISLITDEKIINSNLTAIP
                     ITEERLIFVTNKEMKDFKLNDIQNHTLLVTEKGCSYREQLEMILYKYSFSTKQMIEFL
                     GIESLKKHLKNSGGIALLPEFIVADELENKSLFQIDVDVDIPNLETTLIINPESNKQV
                     LESFVKDVFL"
     gene            complement(2455..>2571)
                     /locus_tag="O0293_002658"
     CDS             complement(2455..>2571)
                     /locus_tag="O0293_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4865 family protein"
                     /protein_id="HCY0281483.1"
                     /translation="IIIYNPDKWKFVKYSFIENLPKSLNPNIKIYDVLYISQ"
BASE COUNT          952 a          351 c          376 g          892 t
ORIGIN      
        1 gaatgagttg gtttatctac atgcaatcta aataacataa aaatgctgat tagaaacatc
       61 attatagtca tcgctataat tagagtgaat ccatttattt tatataatat tcctgataat
      121 ccacctgcaa taaacatacc tgtttgcaaa ctaatctcta atagagaatt tgcatctgta
      181 tattgatctg gttttaatat ctgtttaact aaacttctag atgttgccat atacgtagtc
      241 caacctatcc cattaacaat cgcaaatcca atcactaaat aggtctcaaa tcctatcatt
      301 acgagtgcta tgacaatgag taaatataat attacctgaa gaagataggt gattaaaata
      361 atatttcgtc tattatattt atcggctagt ccacctatta taggagaagc taagaaaccg
      421 gataatacat ttaaagctaa cataatacct aatagttggg agtcgttagt tttgtcgatt
      481 aaataccaat tagccccaac tgtactaatt ccaactccga atgctgaaat tatttctgct
      541 aaaaagaaca atctgaagtt tttaattttc aacaaatcag tcataaatat cacctttttt
      601 caatgcaaga tttatatttg cgagctcttc tataaattga gagtcgttta aaatatcgaa
      661 tggtttattg ccttcgccga tcaaaaatct atagttagta atattcataa actcaaatat
      721 taatttgaat tggttgacta aaggttgtgc tttggtatgc ggattgtcgc cacctatgat
      781 taaaatcaaa tacttttttt gtgacatgat ttctttgaag ttgtctattt gcgtatctcg
      841 caatgactcg gtccatctat caatgaagag ttttaaagac gcactcatgg agtaccaata
      901 gagtggtgtt gaaaaaataa taatatctga ttctaaaact ttgattaaaa tttgttcgta
      961 atcatcatta tgaaagttgg cacctttgct atgacgatta tctgtaacct tttcaatgtt
     1021 gctttgatat aaattcacaa agttgacttt taaattcttc aatagattct ctactgcgat
     1081 agctgaattg ccacctttct actacttcca aataaacaag taatcatagt cataactcct
     1141 ttcgatttac ctttataata atatttaata tattattatt aaatcagaat tcttagaatt
     1201 caggattcga taaaagggaa ccctaaagga ggtactactt tgaacttaaa cattttaaaa
     1261 tcatttttag taactagtga aacaaagaac cttactaaag catcagagtt acttaactat
     1321 tcacagtcaa ctgtatctac acatattgaa aaattagaaa ggcaattaga tgttaaatta
     1381 ttttatagaa aaaaatatgg tatggaacta acggaagaag gcttagcata cgttaaatac
     1441 gctaaagtga ttttagatag taatagcgaa tacgagagag aaataaaagg actttacaat
     1501 aagaaggtaa atataagtat taacatgcaa gaaagtcagt atttgtatcg ctactataat
     1561 aagattagtg aatggttagc tgaacaccca tatgtaaact tgaagtttaa atccgcacat
     1621 tctaatttct atattaaaga agaaattgct aatttcaaat cggatattag ccttatcaca
     1681 gacgaaaaga ttattaatag taacttaact gctattccta taactgaaga acgtttaata
     1741 tttgttacta acaaagagat gaaagatttc aaattaaacg atattcaaaa ccatacatta
     1801 cttgttactg aaaaagggtg tagttacaga gaacagttag aaatgatttt atataaatat
     1861 agcttttcaa caaaacaaat gattgaattt ctaggaattg agtcattaaa aaaacacttg
     1921 aaaaattcag gtgggattgc gctattgccg gaatttattg ttgcagatga attagaaaat
     1981 aaaagtttgt ttcaaataga cgtagatgtc gatattccga atttagaaac aacattgata
     2041 attaatcctg aatcgaataa gcaagtactt gaatcttttg taaaagatgt ttttttataa
     2101 ttattggtga aaacgtgtag ttatggtgaa actcaaagat aataatttaa atgagatgtt
     2161 aatgaaaaag taattcaata taaaaacagg tgatttatat ctttaataag gattatttct
     2221 agttgaaatt tcaaattgcg ggcattcatt ttcaaaatag taaagaattt aatagatgaa
     2281 aacctttgaa tcatactagt tagtatggat tgattaatat tttaacctgc taaatgagta
     2341 gcttatgtat tttaaatagc tgtcatctag caggttcttt attattaata agttcagata
     2401 aattatttta gtctgttttc taatgttgat aataataact ttaaatctgt actttcattg
     2461 tgagatatat agtacatcat aaatttttat atttggattg agactctttg gaagattttc
     2521 aataaatgaa tatttcacaa acttccactt atcaggatta taaattataa t
//
LOCUS       DAKTIO010000245         2551 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.252, whole genome shotgun sequence.
ACCESSION   DAKTIO010000245 DAKTIO010000000
VERSION     DAKTIO010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.252"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            82..1152
                     /locus_tag="O0293_002659"
     CDS             82..1152
                     /locus_tag="O0293_002659"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCY0281484.1"
                     /translation="MTKENICIVFGGKSAEHEVSILTAQNVLNAIDKDKYHVDIIYIT
                     NDGDWRKQNNITAEIKSTDELHLENGEALEISQLLKESSSGQPYDAVFPLLHGPNGED
                     GTIQGLFEVLDVPYVGNGVLSAASSMDKLVMKQLFEHRGLPQLPYISFLRSEYEKYEH
                     NILKLVNDKLNYPVFVKPANLGSSVGISKCNNEAELKEGIKEAFQFDRKLVIEQGVNA
                     REIEVAVLGNDYPEATWPGEVVKDVAFYDYKSKYKDGKVQLQIPADLDEDVQLTLRNM
                     ALEAFKATDCSGLVRADFFVTEDNQIYINETNAMPGFTAFSMYPKLWENMGLSYPELI
                     TKLIELAKERHQDKQKNKYKID"
     gene            1167..2525
                     /locus_tag="O0293_002660"
     CDS             1167..2525
                     /locus_tag="O0293_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCY0281485.1"
                     /translation="MINVTLKQIQSWIPCEIEDQFLNQEINGVTIDSRAISKNMLFIP
                     FKGENVDGHRFVSKALQDGAGAAFYQRGTPIDENVSGPIIWVEDTLTALQQLAQAYLR
                     HVNPKVIAVTGSNGKTTTKDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEI
                     SILEMGMSGFHEIEFLSNLAQPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDN
                     GTFIYDGDEPLLKPHVKEVENAKCISIGVATDNALVCSVDDRDTTGISFTINNKEHYD
                     LPILGKHNMKNATIAIAVGHELGLIYNTIYQNLKNVSLTGMRMEQHTLENDITVINDA
                     YNASPTSMRAAIDTLSTLTGRRILILGDVLELGENSKEMHIGVGNYLEEKHIDVLYTF
                     GNEAKYIYDSGQQHVEKAQHFNSKDDMIEVLINDLKAHDRVLVKGSRGMKLEEVVNAL
                     IS"
BASE COUNT          939 a          342 c          505 g          765 t
ORIGIN      
        1 aaatcaatta acaataggac taaatcaata ttaatttata ttaaggtagc aaaccctgat
       61 atatcattgg aggaaaacga aatgacaaaa gaaaatattt gtatcgtttt tggagggaaa
      121 agtgcagaac acgaagtatc gattctgaca gcacaaaatg tattaaatgc aatagataaa
      181 gacaaatatc atgttgatat catttatatt accaatgatg gtgattggag aaagcaaaat
      241 aatattacag ctgaaattaa atctactgat gagcttcatt tagaaaatgg agaggcgctt
      301 gagatttcac agctattgaa agaaagtagt tcaggacaac catacgatgc agtattccca
      361 ttattacatg gtcctaatgg tgaagatggc acgattcaag ggctttttga agttttggat
      421 gtaccatatg taggaaatgg tgtattgtca gctgcaagtt ctatggacaa acttgtaatg
      481 aaacaattat ttgaacatcg agggttacca cagttacctt atattagttt cttacgttct
      541 gaatatgaaa aatatgaaca taacatttta aaattagtaa atgataaatt aaattaccca
      601 gtctttgtta aacctgctaa cttagggtca agtgtaggta tcagtaaatg taataatgaa
      661 gcggaactta aagaaggtat taaagaagca ttccaatttg accgtaagct tgttatagaa
      721 caaggcgtta acgcacgtga aattgaagta gcagttttag gaaatgacta tcctgaagcg
      781 acatggccag gtgaagtcgt aaaagatgtc gcgttttacg attacaaatc aaaatataaa
      841 gatggtaagg ttcaattaca aattccagct gacttagacg aagatgttca attaacgctt
      901 agaaatatgg cattagaggc attcaaagcg acagattgtt ctggtttagt ccgtgctgat
      961 ttctttgtaa cagaagacaa ccaaatatat attaatgaaa caaatgcaat gcctggattt
     1021 acggctttca gtatgtatcc aaagttatgg gaaaatatgg gcttatctta tccagaattg
     1081 attacaaaac ttatcgagct tgctaaagaa cgtcaccagg ataaacagaa aaataaatac
     1141 aaaattgact aactgaggtt gttattatga ttaatgttac attaaagcaa attcaatcat
     1201 ggattccttg tgaaattgaa gatcaatttt taaatcaaga gataaatgga gtcacaattg
     1261 attcacgagc aatttctaaa aatatgttat ttataccatt taaaggtgaa aatgttgacg
     1321 gtcatcgctt tgtctctaaa gcattacaag atggtgctgg ggctgctttt tatcaaagag
     1381 ggacacctat agatgaaaat gtaagcgggc ctattatatg ggttgaagac acattaacgg
     1441 cattacaaca attggcacaa gcttacttga gacatgtaaa ccctaaagta attgccgtca
     1501 cagggtctaa tggtaaaaca acgactaaag atatgattga aagtgtattg cataccgaat
     1561 ttaaagttaa gaaaacgcaa ggtaattaca ataatgaaat tggtttacct ttaactattt
     1621 tggaattaga taatgatact gaaatatcaa tattggagat ggggatgtca ggtttccatg
     1681 aaattgaatt tctgtcaaac ctcgctcaac cagatattgc agttataact aatattggtg
     1741 agtcacatat gcaagattta ggttcgcgcg aggggattgc taaagctaaa tctgaaatta
     1801 caataggtct aaaagataat ggtacgttta tatatgatgg cgatgaacca ttattgaaac
     1861 cacatgttaa agaagttgaa aatgcaaaat gtattagtat tggtgttgct actgataatg
     1921 cattagtttg ttctgttgat gatagagata ctacaggtat ttcatttacg attaataata
     1981 aagaacatta cgatctgcca atattaggaa agcataatat gaaaaatgcg acgattgcca
     2041 ttgcggttgg tcatgaatta ggtttgatat ataacacaat ctatcaaaat ttaaaaaatg
     2101 tcagcttaac tggtatgcgt atggaacaac atacattaga aaatgatatt actgtgataa
     2161 atgatgccta taatgcaagt cctacaagta tgagagcagc tattgataca ctgagtactt
     2221 tgacagggcg tcgcattcta attttaggag atgttttaga attaggtgaa aatagcaaag
     2281 aaatgcatat cggtgtaggt aattatttag aagaaaagca tatagatgtg ttgtatacgt
     2341 ttggtaatga agcgaagtat atttatgatt cgggccagca acatgtcgaa aaagcacaac
     2401 acttcaattc taaagacgat atgatagaag ttttaataaa cgatttaaaa gcgcatgacc
     2461 gtgtattagt taaaggatca cgtggtatga aattagaaga agtggtaaat gctttaattt
     2521 catagagatt agtcgaggga ccttttactt a
//
LOCUS       DAKTIO010000246         2516 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.253, whole genome shotgun sequence.
ACCESSION   DAKTIO010000246 DAKTIO010000000
VERSION     DAKTIO010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.253"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            595..1506
                     /gene="rapZ"
                     /locus_tag="O0293_002661"
     CDS             595..1506
                     /gene="rapZ"
                     /locus_tag="O0293_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCY0281486.1"
                     /translation="MDNNEKEKSKSELLVVTGLSGAGKSLVIQCLEDMGYFCVDNLPP
                     VLLPKFVELMEQGNPSLRKVAIAIDLRGKELFNSLVAVVDKVKSESDVIIDVMFLEAS
                     TEKLISRYKETRRAHPLMEQGKRSLINAINDEREHLSQIRSIANFVIDTTKLSPKELK
                     ERIRRYYEDEEFETFTINVTSFGFKHGIQMDADLVFDVRFLPNPYYVVDLRPLTGLDK
                     DVYNYVMKWKETEIFFEKLTDLLDFMIPGYKKEGKSQLVIAIGCTGGQHRSVALAERL
                     GNYLNEVFEYNVYVHHRDAHIESGEKK"
     gene            1503..2498
                     /locus_tag="O0293_002662"
     CDS             1503..2498
                     /locus_tag="O0293_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HCY0281487.1"
                     /translation="MRQIKVVLIGGGTGLSVMARGLREFPIDITAIVTVADNGGSTGK
                     IRDEMDIPAPGDIRNVIAALSDSESVLSQLFQYRFEENQISGHSLGNLLIAGMTNITN
                     DFGHAIKALSKILNIKGRVIPSTNTSVQLNAVMEDGEIVFGETNIPKKHKKIDRVFLE
                     PNDVQPMEEAIDALREADLIVLGPGSLYTSVISNLCVNGISDALIHSDAPKLYVSNVM
                     TQPGETDGYSVKDHIDAIHRQAGQPFIDYVICSTQTFNAQVLKKYEEKHSKPVEVNKA
                     ELEKESINVKTSSNLVEISENHLVRHNTKVLSTMIYDIALELISTIPFVPSDKRK"
BASE COUNT          868 a          364 c          491 g          793 t
ORIGIN      
        1 ggaatacatg agtaaaactc atgcataaga aatactaatt tctatagaaa aagtattact
       61 ttatcgttgt cccaccccaa cttgcacatt attgtaagct gactttccgc cagcttctgt
      121 gttggggccc cgccaacttg cacattattg taagctgact tttcgtcagc ttctgtgttg
      181 gggccccgcc aacttgcatt gtctgtagaa attgggaatc caatttctct atgttggggc
      241 ccacacccca actcgcattg cctgtagaat ttcttttcga aattctctgt gttggggccc
      301 acaccccaac tcgcattgcc tgtagaattt cttttcgaaa ttctctgtgt tggggcccct
      361 gactagagtt gaaaaaagct tgttgcaagc gcattttcat tcagtcaact actagcaata
      421 taatattata gaccctagga cattgattta tgtcccaagc tccttttaaa tgatgtatat
      481 ttttagaaat ttaatctaga catagttgga aataaatata aaacatcgtt gcttaatttt
      541 gtcatagaac atttaaatta acatcatgaa attcgttttg gcggtgaaaa aataatggat
      601 aataatgaaa aagaaaaaag taaaagtgaa ctattagttg taacaggttt atctggcgca
      661 ggtaaatctt tggttattca atgtttagaa gacatgggat atttttgtgt agataatcta
      721 ccaccagtgt tattgcctaa atttgtagag ttgatggaac aaggaaatcc atccttaaga
      781 aaagtggcaa ttgcaattga tttaagaggt aaggaactat ttaattcatt agttgcagta
      841 gtggataaag tcaaaagtga aagtgacgtc atcattgatg ttatgttttt agaagcaagt
      901 actgaaaaat taatttcaag atataaggaa acgcgtcgtg cacatccttt gatggaacaa
      961 ggtaaaagat cgttaatcaa tgcaattaat gatgagcgag agcatttgtc tcaaattaga
     1021 agtatagcta attttgttat agatactaca aagttatcac ctaaagaatt aaaagaacgc
     1081 attcgtcgat actatgaaga tgaagagttt gaaactttta caattaatgt cacaagtttc
     1141 ggttttaaac atgggattca gatggatgca gatttagtat ttgatgtacg atttttacca
     1201 aatccatatt atgtagtaga tttaagacct ttaacaggat tagataaaga cgtttataat
     1261 tatgttatga aatggaaaga gacggagatt ttctttgaaa aattaactga tttgttagat
     1321 tttatgatac ccgggtataa aaaagaaggg aaatctcaat tagtaattgc catcggttgt
     1381 acgggtggac aacatcgatc tgtagcatta gcagaacgac taggtaatta tctaaatgaa
     1441 gtatttgaat ataatgttta tgtgcatcat agggacgcac atattgaaag tggcgagaaa
     1501 aaatgagaca aataaaagtt gtacttatcg gtggtggcac tggcttatca gttatggcta
     1561 ggggattaag agaattccca attgatatta cggcgattgt aacagttgct gataatggtg
     1621 ggagtacagg gaaaatcaga gatgaaatgg atataccagc accaggagac atcagaaatg
     1681 tgattgcagc tttaagtgat tctgagtcag ttttaagcca actttttcag tatcgctttg
     1741 aagaaaatca aattagcggt cactcattag gtaatttatt aatcgcaggt atgactaata
     1801 ttacgaatga tttcggacat gccattaaag cattaagtaa aattttaaat attaaaggta
     1861 gagtcattcc atctacaaat acaagtgtgc aattaaatgc tgttatggaa gatggagaaa
     1921 ttgtttttgg agaaacaaat attcctaaaa aacataaaaa aattgatcgt gtgtttttag
     1981 aacctaacga tgtgcaacca atggaagaag caatcgatgc tttaagggaa gcagatttaa
     2041 tcgttcttgg accagggtca ttatatacga gcgttatttc taacttatgt gtgaatggta
     2101 tttcagatgc gttaattcat tctgatgcgc ctaagctata tgtttctaat gtgatgacgc
     2161 aacctgggga aacagatggt tatagcgtga aagatcatat cgatgcgatt catagacaag
     2221 ctggacaacc gtttattgat tatgtcattt gtagtacaca aactttcaat gctcaagttt
     2281 tgaaaaaata tgaagaaaaa cattctaaac cagttgaagt taataaggct gaacttgaaa
     2341 aagaaagcat aaatgtaaaa acatcttcaa atttagttga aatttctgaa aatcatttag
     2401 taagacataa tactaaagtg ttatcgacaa tgatttatga catagcttta gaattaatta
     2461 gtactattcc tttcgtacca agtgataaac gtaaataata tagaacgtaa tcatat
//
LOCUS       DAKTIO010000247         2507 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.254, whole genome shotgun sequence.
ACCESSION   DAKTIO010000247 DAKTIO010000000
VERSION     DAKTIO010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.254"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..510
                     /locus_tag="O0293_002663"
     CDS             <1..510
                     /locus_tag="O0293_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCY0281488.1"
                     /translation="YWYLYTGIMLIAGISYVFYQRDIESKRLLTSIGVGIITAIILIM
                     LQLLFSLITSNLSYSSLIKELARTGVNWKWQMLVTLLFVIPCHELYMRTVLQKELTHF
                     SIPKWVAILITAICSSSLFIYLDNWWIVTFIFVAQVILSLSYEYTRRIATTSVAQIVA
                     IILLLIFNA"
     gene            743..888
                     /locus_tag="O0293_002664"
                     /pseudo
     CDS             743..888
                     /locus_tag="O0293_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
     gene            1427..1528
                     /locus_tag="O0293_002665"
     CDS             1427..1528
                     /locus_tag="O0293_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281489.1"
                     /translation="MKFAANKLTLTIFRKFCINNEKEIWIKYEEVFL"
     gene            1538..1810
                     /locus_tag="O0293_002666"
     CDS             1538..1810
                     /locus_tag="O0293_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HCY0281490.1"
                     /translation="MFGTILYLTLALGLSTAAYASTEYAEGGTWSHGVGSKYVWSYYY
                     HGHKGHGATAIGKYRSFSGYTRAGVKAKASATKHNWWVNRAYYNIY"
     gene            1854..>2507
                     /locus_tag="O0293_002667"
     CDS             1854..>2507
                     /locus_tag="O0293_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HCY0281491.1"
                     /translation="MKWFKLILDVTIFILIAILLFVYTYKENEEILPDTKYPIAVTDW
                     NKKYSKNEIYKRIDQFAKNENVAIYKSTSNYTNKNVDKDIYVFNKSKAATITPFNAKY
                     NIHYLSDDEVLKKDIKGSYFVKDKNFDVSKFINFLKEYGVTAESYKIDHMMIAVGVIK
                     QMNIVVPLSSLLIVYFIYYIFEKNINFKAYAIKYLNGFTLRKIIFENFSKKCTYWVTL
                     "
BASE COUNT          908 a          309 c          396 g          894 t
ORIGIN      
        1 tactggtatt tatatacagg tataatgcta attgctggta tcagttatgt attttatcaa
       61 agagatattg aatctaagcg gttgcttaca tcaattggtg ttggtattat tacggcaatt
      121 attttaatta tgcttcaact tttattctca cttataactt ctaatttaag ttatagttca
      181 ttaattaaag aattagcaag aacaggtgtc aattggaagt ggcaaatgtt agtaacttta
      241 ctttttgtca ttccatgtca cgagttatat atgagaactg ttttacaaaa agaattaaca
      301 catttttcaa taccgaaatg ggtagctata ttaataactg caatttgttc tagttcatta
      361 tttatttatt tagataattg gtggatagta acatttatat ttgttgcaca agtgatatta
      421 tcattaagtt atgagtatac tcgacgtata gcaacaacat ccgttgcaca aattgtagct
      481 attatattgt tattaatttt taatgcttaa ataagctttt cttagacgta tgtgttctaa
      541 gaaaagcttt tttgcttaag aatagcaata cgaagattcg aggcatgctt cattatagtg
      601 gtaatatttt ttaacagcaa gcggttatta ttgagattag gttttagtca gtttttatta
      661 ctaaatattt tataaaaatg gtataatatt tttgagcaag ttgaattgat ggtggctatc
      721 tgagtaaagg ggggtggtgc ctatggcatt acttaacact tttgaaagga aaagcctatt
      781 gtgatatcta ttgcaaacgc attacattta atgttaagtt tcggtatgtt tatcgtcact
      841 ttcattggta tagtagtagc aataataaat ttaagcaata aaaaataacc atcatttcaa
      901 aactttgaca tagaatgatg gttaaagaaa atctcattta gtcaccgtct ttttaacggg
      961 ctcattaggc gacatgttcc tgcatgtcgt ctttttacat ttttatagta acatatcttt
     1021 tatttaattg tctattttta ttagatgtgt aacaaaattt tgaagtattt tccgtatatt
     1081 gcctgctaaa catgtcagat ttgaaaggaa aatcatactg tggtagaaat caattatgat
     1141 tatatggtag tacttataaa taagtgattt ctattgaata tgtaatgaag ttcatatatt
     1201 aatataactt tcattgatat agaagtcgca atgattaatt taaacaataa aaaacaacca
     1261 tcatttccaa actttgacaa cgaatgatgg ttaaagaaaa tctcatttag tcaccgtctt
     1321 tttaacgggc tcaataggcg acatgttcct gcatatcgtc tatttataca ctgcacaatt
     1381 taataataat acaattatac tttgatgtcc atttaacata aagtggttga aatttgcagc
     1441 aaataaatta accttgacta ttttcagaaa attctgtata aataacgaaa aagagatatg
     1501 gattaaatat gaagaggtgt ttttatagaa gaataaaatt tttggcacta tattatattt
     1561 aactttagca cttggattat caacagcagc ttatgcatct acagaatacg cagaaggagg
     1621 cacttggagt catggtgtcg gcagtaagta tgtttggtcg tattattatc atggtcataa
     1681 aggacatggt gcaacagcta ttggaaaata tagatcattt agtggttata caagagctgg
     1741 tgtaaaagca aaagcatcag ctactaaaca taattggtgg gtcaatagag cgtattataa
     1801 catttattaa atactaaaaa atcatagaaa ataaacaacg caagaggtac aacatgaaat
     1861 ggttcaaatt aatacttgat gttacaatct tcattctgat tgctatattg ttatttgttt
     1921 atacatataa agaaaacgaa gaaatattgc ctgatactaa ataccctata gcggtaactg
     1981 actggaataa aaaatatagt aaaaatgaga tttataaacg tatagatcaa ttcgctaaaa
     2041 atgagaacgt agcaatctat aaatcaactt caaattatac aaacaaaaac gtagataaag
     2101 atatatatgt atttaataaa tcaaaagcag caactatcac tccttttaat gctaaatata
     2161 acattcatta tttaagtgac gacgaagtat taaaaaaaga tatcaaaggg agttattttg
     2221 taaaagacaa aaattttgac gtgtctaaat tcataaattt tttaaaagaa tatggtgtta
     2281 ctgctgaatc atacaaaata gatcatatga tgattgccgt tggtgtcatt aaacaaatga
     2341 atatagtagt tccattatcc tcacttttaa tcgtttattt tatttattat attttcgaaa
     2401 agaacattaa tttcaaagcg tatgcgatta agtatttaaa tggttttaca ttaagaaaaa
     2461 taattttcga aaatttttca aagaaatgca cgtattgggt aacgtta
//
LOCUS       DAKTIO010000248         2480 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.255, whole genome shotgun sequence.
ACCESSION   DAKTIO010000248 DAKTIO010000000
VERSION     DAKTIO010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.255"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(102..203)
                     /locus_tag="O0293_002668"
     CDS             complement(102..203)
                     /locus_tag="O0293_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281492.1"
                     /translation="MQGFKEKHQELKKALCQIGLMRSISEVKQLNIA"
     gene            complement(326..421)
                     /gene="pepA1"
                     /locus_tag="O0293_002669"
     CDS             complement(326..421)
                     /gene="pepA1"
                     /locus_tag="O0293_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin
                     PepA1"
                     /protein_id="HCY0281493.1"
                     /translation="MMLIFVHIIAPVISGCAIAFFSYWLSRRNTK"
     gene            624..767
                     /locus_tag="O0293_002670"
     CDS             624..767
                     /locus_tag="O0293_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCY0281494.1"
                     /translation="MTRERRSFSSEFKLQMVRLYKNGKPRNEIIREYDFTPSTFVNGG
                     YKM"
     gene            1371..1754
                     /locus_tag="O0293_002671"
     CDS             1371..1754
                     /locus_tag="O0293_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281495.1"
                     /translation="MSNQNLFDELEKKGYKLEDIFTKEEIKKYKAEDQLRAGKTQYVE
                     TGKDTATLYLSSAYTKTIAALGAGAISVISALTGGLVGAGVGGFLGSIAASNIDTSKG
                     IYIKLKTKKNAAGEYVLTGEKWGYQ"
     gene            1765..1941
                     /locus_tag="O0293_002672"
     CDS             1765..1941
                     /locus_tag="O0293_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281496.1"
                     /translation="MDRLKYSLKVGILALLLFCTLNYLVPMQSNAFSIIIYSAIFAVL
                     LMLLVYIFLGILKK"
     gene            1943..2128
                     /locus_tag="O0293_002673"
     CDS             1943..2128
                     /locus_tag="O0293_002673"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281497.1"
                     /translation="MKQISIGYEEIYGDRIFHSHFLKEHEVKNWKTVNQIIEKEHLDK
                     NEYEAEPPLFFFSGSLD"
     gene            2242..>2480
                     /locus_tag="O0293_002674"
     CDS             2242..>2480
                     /locus_tag="O0293_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281498.1"
                     /translation="MFTPIVINLLNDVGSIFLVRVGNQLKVIEKVHYEDILESKIVRL
                     FQAFIKDKMDQLFITSEHFAIDIDKLLFQLNLNVR"
BASE COUNT          931 a          319 c          430 g          800 t
ORIGIN      
        1 attaattaaa cgtacatctt ttggttatgg aagttacaat catttgcgca atcgaatatt
       61 attatgttca aaactttacg ctccaaaaag taaaaaggaa gttaagcaat gtttagttgc
      121 ttaacttcgg atattgaacg catcagtcca atttgacata gagccttttt tagttcttga
      181 tgtttctctt taaaaccttg catattttac aaaaagaaaa attagcagta taattaagac
      241 aacgaaaata agtatttact tatacaccaa tcccctcact atttgcggta gtgaggggat
      301 ttttattggt gcggctatat gtcacctatt ttgtattgcg tctacttagc caataagaaa
      361 aaaacgcaat ggcacagcca ctgatgactg gtgctatgat gtgaacgaaa ataagcatca
      421 ccttatacac ctcctctctg cgtctaaatt gacgcctgag agataggcga ctctactatt
      481 atatcatcgg caaatataca agcacagtca cttgcttctg ataagttata tgattctagc
      541 tgatagattg aatcgtctac acttaattgg acaaattcta tgagaataga tattgttaat
      601 ttaagaaagt aggcgatttt attatgacaa gagaaagaag atcatttagt tcagagttta
      661 agttacaaat ggttagatta tataaaaatg gtaagcctag gaatgaaatt atacgcgagt
      721 atgatttcac accttcgacg tttgtaaatg gcggttataa aatgtaggaa aatggataaa
      781 gcaacatcaa aacacgggta cattcaatca ccaagataac ttatcggatg aagaaaaaga
      841 gctgattaaa ttacgcaaag aagttcaaca tttaaaaatg gagaacgata ttttaaagca
      901 agtagcgctg attatggggc aaaaatagaa gtcattcaaa agaatgcaca tcaatattca
      961 gtatcagcaa tgtgtaaagt cctgataata ctaagaagta cctattatga ttctataaaa
     1021 agaaaagata ataaaatcac taaagatgat tcaaacatag aacatgccgt cataaatatt
     1081 tttaattcta atagaaaagt ctttggtaca agacgaatta aaaatcattt aaatgacaag
     1141 ggtctcactg tatctggaca aaagataggt cgatcatgaa aaaatctagt ttctgtttat
     1201 acgaaatcta aatacaaaaa tcatctaaaa gaaactaatg aaaaacgaat taaaaatctt
     1261 tattattagc tgctggtgta ttatttgtta gtccaatttc attatcttat aattcagatg
     1321 tagctcatgc tgaagataag ttagaccatt ctcaagcaaa ggtaatatat ttgagtaacc
     1381 aaaatttatt tgatgaactt gagaaaaaag gttataaact ggaagatata tttacaaaag
     1441 aagaaataaa aaaatataaa gctgaagacc aattgagagc gggtaaaact caatatgtag
     1501 aaacaggtaa agatactgca acattatatc tttcttctgc atatacaaaa acaatagctg
     1561 ctttaggtgc gggggctatt agcgttattt ctgcactaac aggaggttta gttggcgcag
     1621 gtgttggtgg tttccttgga tctatagctg catcgaatat tgatactagt aagggaatat
     1681 atataaaatt aaaaactaaa aagaatgcag ccggggaata cgttctgaca ggagaaaaat
     1741 ggggatatca gtaagggtga ttttatggat agattaaaat attcacttaa agttggaatt
     1801 ttagcattat tattattttg tactttaaat tatttagttc caatgcaaag caatgctttt
     1861 tcaataatta tatattcggc aatttttgct gtgttactta tgcttttagt ttatatattt
     1921 ttaggaattt taaagaaatg acatgaaaca aattagcatt ggctatgaag aaatctatgg
     1981 ggatagaatt tttcatagcc attttttaaa agagcatgaa gtaaaaaatt ggaaaaccgt
     2041 caatcaaata attgaaaaag aacacttgga caaaaatgaa tatgaagcgg aaccgccgtt
     2101 atttttcttt tcaggatcgt tagattagat tcggttgtat agtataatga aaaatattaa
     2161 aataaattta taaaaaaggt attttatttt gaaatcatgt cccttcgtta catatagtag
     2221 tagaggggtt ttattttaat aatgtttaca cctatagtta taaatttatt aaatgatgta
     2281 ggttcgattt ttttagttag ggtggggaat caattgaaag ttattgagaa agttcattat
     2341 gaagatattt tagaatcaaa aatagttcga ttatttcagg catttataaa ggacaaaatg
     2401 gatcaacttt ttattacttc tgaacacttt gctattgaca tagacaagct attatttcaa
     2461 cttaatttaa atgttagata
//
LOCUS       DAKTIO010000249         2460 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.256, whole genome shotgun sequence.
ACCESSION   DAKTIO010000249 DAKTIO010000000
VERSION     DAKTIO010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.256"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..196)
                     /locus_tag="O0293_002675"
     CDS             complement(<1..196)
                     /locus_tag="O0293_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCY0281499.1"
                     /translation="MNKPFIAIEGPIGVGKSSLAHKLSQTLDFYEEKEIITENPFLSD
                     FYEDISKWSFQTEMFFLCNRY"
     gene            263..733
                     /locus_tag="O0293_002676"
     CDS             263..733
                     /locus_tag="O0293_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HCY0281500.1"
                     /translation="MTNDIYFMTLAIEEAKKAAQLGEVPIGAIITKDDEVIARAHNLR
                     ETLQQPTAHAEHIAIERAAKVLGSWRLEGCTLYVTLEPCVMCAGTIVMSRIPRVVYGA
                     DDPKGGCSGSLMNLLQQSNFNHRAIVDKGVLKEACSTLLTTFFKNLRANKKSTN"
     gene            880..1749
                     /locus_tag="O0293_002677"
     CDS             880..1749
                     /locus_tag="O0293_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCY0281501.1"
                     /translation="MIKLIATDMDGTLLNAAHEISQPNIDAIKYAQEQGITVVIATGR
                     AFYEAQAPVADTDLTVPYICLNGAEVRDETFNVMSTSHLNKSLVHKITNVLKDAGIYY
                     QVYTSRAIYTEDPQRDLDIYIDIAERAGQHANVERIKNGIQRRIDNGTLKVVDNYDAI
                     ENIPGELIMKILAFDGNLEKIDKASKILAESPNLAISSSSRGNIEITHSDAQKGIALE
                     TIAERLGIEMKEVMSIGDNLNDLSMLEKVGYPVAMENGAEEVKKIAKYVTDTNENSGV
                     GKAIMKLLREQQV"
     gene            1770..2336
                     /locus_tag="O0293_002678"
     CDS             1770..2336
                     /locus_tag="O0293_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCY0281502.1"
                     /translation="MKGLIIIGSAQVNSHTSALARYLTEHFKTHDIEAEIFDLAEKPL
                     NQLDFSGTTPSIDEIKQNMKDLKEKAMAADFLILGTPNYHGSYSGILKNALDHLNMDY
                     FKMKPVGLIGNSGGIVSSEPLSHLRVIVRSLLGIAVPTQIATHDSDFAKNEDGSYYLN
                     DSEFQLRARLFVDQIVSFVNNSPYEHLK"
BASE COUNT          931 a          335 c          448 g          746 t
ORIGIN      
        1 tatatctatt gcataaaaag aacatttcag tttgaaaact ccatttagag atatcttcat
       61 aaaagtctga taaaaatgga ttttctgtga tgatttcttt ttcttcataa aaatctaaag
      121 tttgacttaa tttgtgtgca agtgaagatt tacctacgcc aataggacct tcaattgcta
      181 taaaaggttt gttcatattc acatctccaa atgttaaaat agacattgag tattgtacca
      241 taagtgaggt atctggacat aaatgacaaa tgatatatat tttatgacat tagcgattga
      301 agaagctaaa aaagcagctc aactaggcga agtacctata ggtgctatca tcactaaaga
      361 tgatgaagtt atcgctagag cacataattt aagagaaaca ctacaacaac caacggcgca
      421 tgctgaacat attgcaattg aacgtgcagc caaagtgtta ggtagttggc gtttagaagg
      481 ttgcacatta tatgtaacct tagaaccatg tgtcatgtgc gcaggaacaa ttgtaatgag
      541 tcgcattcca agagtcgtct atggcgcaga tgatcctaaa ggtggttgta gtggcagttt
      601 aatgaattta ttgcaacaat ctaattttaa tcatcgtgca attgttgata aaggtgtact
      661 taaagaagca tgtagcacat tattaacaac attttttaaa aacttaagag ccaataagaa
      721 atccaccaat tagacgatag tacatactaa ttaaaatttg ttagaattac attatatgat
      781 aaattaatga caatataaat gttaatagaa aagtatgtgc gctgagtata tattcttatt
      841 taagaacaag attttaaatt tacgaaacga ggtaacataa tgataaaact aatagccact
      901 gatatggatg gcacgctact taatgcagca catgaaattt ctcaacctaa tattgatgcg
      961 attaaatacg ctcaagaaca agggataacg gttgttatcg cgacaggtcg agcattttat
     1021 gaagcacaag caccagttgc tgacacagat ttaacagtac catatatttg tttgaatggt
     1081 gctgaagtac gtgatgaaac tttcaatgta atgagcactt cacaccttaa taaatcgtta
     1141 gtacacaaaa ttacaaatgt tttaaaagat gcaggtattt attatcaagt atacacgagt
     1201 cgtgcgattt atactgaaga tccacaaaga gatttagaca tttacataga tattgctgag
     1261 cgtgcaggtc aacatgcaaa cgttgagcgt attaaaaatg gtattcaaag acgcatagat
     1321 aatggtacgt tgaaagttgt tgataattat gatgctattg aaaacatacc tggtgaatta
     1381 attatgaaaa tattagcatt tgatggaaat ttagaaaaaa ttgacaaagc tagtaaaatt
     1441 ttagctgaat ctccgaattt agctatatca tcatcttcga gaggaaatat agaaataacg
     1501 cattcagatg cacaaaaagg tattgcgcta gaaacaattg ccgaaagatt agggattgaa
     1561 atgaaagaag tcatgtcaat aggtgacaat ttaaatgact tatcaatgtt agagaaagtt
     1621 ggctatccag ttgcgatgga aaatggtgca gaagaagtta aaaaaatagc gaaatatgtc
     1681 acagatacga atgaaaatag tggtgttgga aaagctatta tgaaattatt acgtgaacaa
     1741 caagtttaat aaaaaaagag gggtcaaata tgaaaggatt aattattatt ggcagtgcac
     1801 aagtgaattc acatacaagt gcactagcaa gatacttaac tgagcatttt aaaacacatg
     1861 atattgaagc ggaaatattc gatttagcag aaaaaccgtt aaatcaatta gatttttcag
     1921 gaacaacacc gtctattgat gaaatcaaac aaaatatgaa agatttaaaa gagaaagcaa
     1981 tggcggcgga ctttttaata ttaggaacgc caaactatca tggttcatat tctggaatat
     2041 tgaaaaatgc attagatcat ctaaatatgg attattttaa aatgaaacct gtaggcttaa
     2101 taggaaatag tggtggtatt gttagttcag agccattgtc acatttaaga gtaatcgtca
     2161 gaagtttact aggcattgct gtaccaactc aaatagcaac acatgattct gattttgcta
     2221 aaaatgaaga tggttcatat tacttaaatg atagtgaatt ccaattacga gcaagattat
     2281 ttgtcgatca aattgtatct tttgtgaata atagtccata tgaacattta aaataatatt
     2341 aaaaaatatg taaatataca ttaaaaatag gtatgtagct taaggtattt cattgagaat
     2401 gcttttaagt cacatgccta ttttttgttc taatgcacca atagtaagcc tttgatatta
//
LOCUS       DAKTIO010000250         2451 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.257, whole genome shotgun sequence.
ACCESSION   DAKTIO010000250 DAKTIO010000000
VERSION     DAKTIO010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.257"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(82..642)
                     /gene="icaR"
                     /locus_tag="O0293_002679"
     CDS             complement(82..642)
                     /gene="icaR"
                     /locus_tag="O0293_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ica operon transcriptional regulator IcaR"
                     /protein_id="HCY0281503.1"
                     /translation="MKDKIIDNAITLFSEKGYDGTTLDDIAKSVNIKKASLYYHFDSK
                     KSIYEQSVKCCFDYLNNIIMMNQNKSNYSIDALYQFLFEFIFDIEERYIRMYVQLSNT
                     PEEFSGNIYGQIQDLNQSLSKEIAKFYDESKIKMTKEDFQNLILLFLESWYLKASFSQ
                     KFGAVEESKSQFKDEVYSLLNIFLKK"
     gene            806..2044
                     /gene="pgaC"
                     /locus_tag="O0293_002680"
     CDS             806..2044
                     /gene="pgaC"
                     /locus_tag="O0293_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HCY0281504.1"
                     /translation="MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVD
                     ELEGITFLLACYNESETIEDTLSNVLALKYEKKEIIIINDGSSDNTAELIYKIKENND
                     FIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAV
                     TGNPRIRNKSSILGKIQTIEYASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGY
                     WDTDMITEDIAVSWKLHLRGYRIKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLL
                     RDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGYLFITANFLDYTFMTYSFSIFL
                     LSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKAL
                     KRKKGGYATWSSPDRGNTQR"
     gene            2008..2313
                     /gene="icaD"
                     /locus_tag="O0293_002681"
     CDS             2008..2313
                     /gene="icaD"
                     /locus_tag="O0293_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular adhesion protein D"
                     /protein_id="HCY0281505.1"
                     /translation="MVKPRQREYPTLKSSLNIVRETALIAISCVFWIYCLVVLLVYIG
                     TIFEIHDESINTIRVALNIENTEILDIFETMGIFAIIIFVFFTISILIQKWQRGRES"
     gene            2310..>2451
                     /locus_tag="O0293_002682"
     CDS             2310..>2451
                     /locus_tag="O0293_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intercellular adhesin biosynthesis
                     polysaccharide N-deacetylase"
                     /protein_id="HCY0281506.1"
                     /translation="MKYRKFIILVLSILIILPVSTLDGHHIANADDDSPKKLKYKENS
                     ALA"
BASE COUNT          834 a          373 c          386 g          858 t
ORIGIN      
        1 caactttata tagatttaaa aaaataatct catgtctttt tttacaaaag taagttaatt
       61 attacaaact agtaacaaaa attatttctt caaaaatata tttagtagcg aatacacttc
      121 atctttgaat tgacttttac tttcttccac tgctccaaat ttttgcgaaa aggatgcttt
      181 caaataccaa ctttcaagaa acagcaatat taaattctga aagtcttctt ttgtcatctt
      241 tatctttgat tcatcataga attttgctat ctctttactt aatgattgat ttaaatcttg
      301 tatttgtccg taaatatttc cagaaaattc ctcaggcgta ttagataatt gaacgtacat
      361 tctaatatac ctttcttcga tgtcgaaaat aaactcaaat aagaattgat ataaagcatc
      421 aattgaatag ttcgatttat tttgattcat cataataata ttattaaggt aatcaaaaca
      481 acatttaaca ctttgttcgt aaatactttt tttcgagtca aaatggtaat ataaactcgc
      541 tttctttata tttacacttt tagctatatc atcaagtgtt gtaccgtcat accccttctc
      601 tgaaaataag gttattgcgt tatcaataat cttatccttc aatttttata accccctact
      661 gaaaattaat cacactatgt tacaggaaaa ttaagttgca attacaaata tttccgttta
      721 attataacaa caatctattg caaattaaaa tactatcaat taccatatgg cttacaacct
      781 aactaacgaa aggtaggtaa agaaattgca attttttaac tttttgcttt tttatcctgt
      841 atttatgtct atttactgga ttgtcggttc aatttatttc tattttacca gagaaattag
      901 atattcattg aacaagaagc ctgacataaa tgtggatgaa ttagaaggca ttacattttt
      961 acttgcctgt tataacgaaa gtgaaacgat tgaagatacg ttgtctaatg ttcttgcact
     1021 caaatacgag aagaaagaaa ttattatcat taatgatgga agttcagata atacagcaga
     1081 actcatctat aaaatcaaag aaaataatga ctttattttc gtcgatttac aagaaaacag
     1141 aggtaaagcc aacgcactca atcaaggcat taaacaggct tcatatgatt atgtaatgtg
     1201 cttggatgca gatactatcg ttgatcaaga tgcaccatat tatatgattg agaatttcaa
     1261 acatgatcca aaacttggtg cagttacagg taatcctaga attcgaaata agagttctat
     1321 tttaggtaaa attcaaacga tagaatatgc aagtttaatt ggctgtatta agcgaagtca
     1381 gacacttgct ggcgcagtca atactatttc gggtgtcttc actctattta aaaaaagtgc
     1441 agttgtcgac gttggctact gggatactga tatgattacc gaagatattg cagtttcttg
     1501 gaaattgcat ttacgtggat atcgtattaa gtatgaaccg cttgccatgt gttggatgtt
     1561 ggttccagaa acattgggag gtctttggaa gcaacgcgtg agatgggctc aagggggaca
     1621 cgaagtatta ctacgagact tttttagcac aatgaaaacg aaaaggtttc ctttatatat
     1681 tttgatgttt gagcaaatca tctcgatttt atgggtatat atagtgcttc tatatttagg
     1741 ctatttgttc ataacagcaa acttcttaga ctatacattt atgacatata gtttttcaat
     1801 atttctacta tcatcattta ctatgacttt tataaacgtt attcaattta cagtcgcact
     1861 ctttattgat agtcgctacg agaaaaagaa tatggctgga ctcatatttg taagttggta
     1921 tccgacagta tactggatta ttaacgcagc agtagttctt gtcgcatttc caaaagcatt
     1981 aaaacgtaag aaaggtggtt acgcaacatg gtcaagccca gacagaggga atacccaacg
     2041 ctaaaatcat cgctaaatat tgtaagagaa acagcactta tcgctatatc ttgtgtcttt
     2101 tggatatatt gtttagttgt tctactcgtt tatattggta ctatatttga aattcatgac
     2161 gaaagtatca atacaatacg tgttgcttta aacattgaaa atactgaaat tttagatata
     2221 tttgaaacta tgggcatttt cgcgattatc atttttgtat tttttacaat tagcatattg
     2281 attcaaaaat ggcagagagg aagagaatcg tgaagtatag aaaatttata attttagtgt
     2341 tgagtatctt gatcatattg cctgtaagca cactggatgg tcatcatatt gcaaatgcag
     2401 atgacgattc acctaaaaaa ctgaaatata aagaaaatag tgctctggca t
//
LOCUS       DAKTIO010000251         2441 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.258, whole genome shotgun sequence.
ACCESSION   DAKTIO010000251 DAKTIO010000000
VERSION     DAKTIO010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.258"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            19..936
                     /gene="truB"
                     /locus_tag="O0293_002683"
     CDS             19..936
                     /gene="truB"
                     /locus_tag="O0293_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCY0281507.1"
                     /translation="MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAG
                     VLPVCIGNATRVSDYVMDMGKAYEATVSIGRSTTTEDQTGDTLETKGVHSADFNKDDI
                     DRLLESFKGIIEQIPPMYSSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDF
                     KENECHFKIRVICGKGTYIRTLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQI
                     KELHEQDSLQNKLFPLEYGLKGLPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVF
                     IDDDSEKVLAIYMVHPTKESEIKPKKVFN"
     gene            951..1922
                     /gene="ribF"
                     /locus_tag="O0293_002684"
     CDS             951..1922
                     /gene="ribF"
                     /locus_tag="O0293_002684"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCY0281508.1"
                     /translation="MKVIEVTHPIQSKQYITEDVAMAFGFFDGMHKGHDKVFDILNEI
                     AEARSLKKAVMTFDPHPSVVLNPKRKRTTYLTPLSDKIEKISQHDIDYCIVVNFSSRF
                     ANVSVEDFVENYIIKNNVKEVIAGFDFTFGKFGKGNMTVLQEYDAFNTTIVSKQEIEN
                     EKISTTSIRQDLINGELQKANDALGYIYSIKGTVVQGEKRGRTIGFPTANIQPSDDYL
                     LPRKGVYAVSIEIGTENKLYRGVANIGVKPTFHDPNKAEVVIEVNIFDFEDNIYGERV
                     TVNWHHFLRPEIKFDGIDPLVKQMNDDKSRAKYLLAVDFGDEVAYNI"
     gene            2037..2306
                     /gene="rpsO"
                     /locus_tag="O0293_002685"
     CDS             2037..2306
                     /gene="rpsO"
                     /locus_tag="O0293_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCY0281509.1"
                     /translation="MAISQERKNEIIKEYRVHETDTGSPEVQIAVLTAEINAVNEHLR
                     THKKDHHSRRGLLKMVGRRRHLLNYLRSKDIQRYRELIKSLGIRR"
BASE COUNT          910 a          332 c          464 g          735 t
ORIGIN      
        1 aaaaaagaag gtgactatat gtataatggg atattaccag tatataaaga gcgcggttta
       61 acaagtcatg acgttgtatt caaattgcgt aaaatattaa aaactaaaaa aataggtcac
      121 acgggtacgc ttgatcccga agttgcaggc gtgttaccgg tatgtatagg taatgcaacg
      181 agagttagtg attatgttat ggatatgggc aaagcttatg aagcaactgt atcgatagga
      241 agaagtacaa cgactgaaga tcaaacgggt gatacattgg aaacaaaagg tgtacactca
      301 gcagatttta ataaggacga tattgaccga ttgttagaaa gttttaaagg tatcattgaa
      361 caaattccgc cgatgtactc atccgtcaaa gtaaatggta aaaaattata tgaatatgcg
      421 cgtaataatg aaacagttga aagaccaaag cgtaaagtta atattaaaga cattgggcgt
      481 atatctgaat tagattttaa agaaaatgag tgtcatttta aaatacgcgt catctgtggt
      541 aaaggtacat atattagaac gctagcaact gatattggtg tgaaattagg ctttccggca
      601 catatgtcga aattaacacg aatcgagtct ggtggatttg tgttgaaaga tagccttaca
      661 ttagaacaaa taaaagaact tcatgagcag gattcattgc aaaataaatt gtttccttta
      721 gaatatggat taaagggttt gccaagcatt aaaattaaag attcgcacat aaaaaaacgt
      781 attttaaatg ggcagaaatt taataaaaat gaatttgata acaaaattaa agaccaaatt
      841 gtatttattg atgatgattc agaaaaagta ttagcaattt atatggtaca ccctacaaaa
      901 gaatcagaaa ttaaacctaa aaaagtcttt aattaaagga gatagaattt atgaaagtca
      961 tagaagtgac acatcctata caatctaaac agtatattac agaggatgtt gcaatggcat
     1021 tcggattttt cgatggcatg cataaaggtc atgacaaagt ctttgatata ttaaacgaaa
     1081 tagctgaggc acgcagttta aaaaaagcgg tgatgacatt tgatccgcat ccgtctgtcg
     1141 tgttgaatcc taaaagaaaa cgaacaacgt atttaacgcc actttcagat aaaatcgaaa
     1201 aaattagcca acatgatatt gattattgta tagtggttaa tttttcatct aggtttgcta
     1261 atgtgagcgt agaagatttt gttgaaaatt atataattaa aaataatgta aaagaagtca
     1321 ttgctggttt tgattttact tttggtaaat ttggaaaagg taatatgact gtacttcaag
     1381 aatatgatgc gtttaatacg acaattgtga gtaaacaaga aattgaaaat gaaaaaattt
     1441 ctacaacttc tattcgtcaa gatttaatca atggtgagtt gcaaaaagcg aatgatgctt
     1501 taggctatat atattctatt aaaggcactg tagtgcaagg tgaaaaaagg ggaagaacta
     1561 ttggcttccc aacagctaac attcaaccta gtgatgatta tttgttacct cgtaaaggtg
     1621 tttatgctgt tagtattgaa atcggcactg aaaataaatt atatcgaggg gtagctaaca
     1681 taggtgtaaa gccaacattt catgatccta acaaagcaga agttgtcatc gaagtgaata
     1741 tctttgactt tgaggataat atttatggtg aacgagtgac cgtgaattgg catcatttct
     1801 tacgtcctga gattaaattt gatggtatcg acccattagt taaacaaatg aacgatgata
     1861 aatcgcgtgc taaatattta ttagcagttg attttggtga tgaagtagct tataatatct
     1921 agagttgcgt atagttatat aaacaatcta taccacacct ttttcttagt aggtcgaatc
     1981 tccaacgcct aactcggatt aaggagtatt caaacatttt aaggaggaaa ttgattatgg
     2041 caatttcaca agaacgtaaa aacgaaatca ttaaagaata ccgtgtacac gaaactgata
     2101 ctggttcacc agaagtacaa atcgctgtac ttactgcaga aatcaacgca gtaaacgaac
     2161 acttacgtac acacaaaaaa gaccaccatt cacgtcgtgg attattaaaa atggtaggtc
     2221 gtcgtagaca tttattaaac tacttacgta gtaaagatat tcaacgttac cgtgaattaa
     2281 ttaaatcact tggtatccgt cgttaatctt aatataacgt ctttgaggtt ggggcatatt
     2341 tatgttccaa ccttaattta tattaaaaaa gctttttaca aatattaaca tttattatat
     2401 gttaagctaa tattgagtga ataataaggt tacaatgaga t
//
LOCUS       DAKTIO010000252         2426 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.259, whole genome shotgun sequence.
ACCESSION   DAKTIO010000252 DAKTIO010000000
VERSION     DAKTIO010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.259"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..422
                     /locus_tag="O0293_002686"
     CDS             <1..422
                     /locus_tag="O0293_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HCY0281510.1"
                     /translation="YQYTGFTKEAINKTQNSGYENEYFYISAIPYNLAEYRDYFEPLL
                     NKSDSEFSKELSNVKKQLKDKSKVSVTTTLFSKKKNYTKKSNSENVIKMAEEIKKDKE
                     IPNGIELSIKFSDNKINTVKPNFNGESTSEYGVFDQE"
     gene            621..740
                     /locus_tag="O0293_002687"
     CDS             621..740
                     /locus_tag="O0293_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281511.1"
                     /translation="MTIFTQLSDRIKKAISKINQANNIPNNARKSPMYHSPIT"
     regulatory      823..957
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1019..1564
                     /locus_tag="O0293_002688"
     CDS             1019..1564
                     /locus_tag="O0293_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCY0281512.1"
                     /translation="MQQNKRLITISMLSAIAFVLTFIKFPIPFLPPYLTLDFSDVPSL
                     LATFTFGPVAGIVVALVKNLLNYLFSMGDPVGPFANFLAGASFLLTAYAIYKNKRSTK
                     SLITGLIIATIVMTIVLSILNYFVLLPLYGMIFNLADIANNLKVIIVSGIIPFNIIKG
                     IVISIVFILLYRRLANFLKRI"
     gene            complement(1670..1918)
                     /locus_tag="O0293_002689"
     CDS             complement(1670..1918)
                     /locus_tag="O0293_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HCY0281513.1"
                     /translation="MAKYTIVDMDTCIACGACGAAAPDIYDYDDEGIAFVILDDNQGT
                     AEVPEELYEDMEDAIDGCPTDSIKIADESFDGDALKFE"
     gene            2026..>2426
                     /locus_tag="O0293_002690"
     CDS             2026..>2426
                     /locus_tag="O0293_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281514.1"
                     /translation="MQHIIKTALQQTFNYKTNKSIYNILVGKKSHQTFFDACSQQQLS
                     LYHSLPLLKYPSFELFLEKINEFNAEMEIMLHPRYTFESMGQTFQAIQLLVQTMSNTK
                     QHVFHFVPISQNNKIQETVKIVYNYIKENQL"
BASE COUNT          896 a          366 c          366 g          798 t
ORIGIN      
        1 aatatcaata tacaggattt acaaaagaag caattaataa gacgcaaaat agtggttatg
       61 aaaatgaata tttttatatt tcggccatac cttataattt agctgagtat agagactatt
      121 ttgaaccatt gttaaacaaa agtgacagtg aattttcaaa agaattgtca aatgttaaga
      181 agcaattaaa agataagtct aaagtttcgg taactactac tctatttagt aaaaaaaaga
      241 actatactaa aaaaagtaac agtgaaaatg taataaaaat ggcagaagaa ataaaaaaag
      301 ataaagagat accaaacggt atagagctta gtataaaatt ttcggacaat aaaataaata
      361 cggttaaacc aaattttaac ggtgaaagca cttcagaata tggtgtgttt gatcaagaat
      421 aaaattaatg atgaaaattt aacggagaat agtgtatatt gagtagatca agaataaaaa
      481 gataattcta ctattgttgt gaaggcaaat aagtagaaga ttttaagtgt aatttctggt
      541 gatttaaata ataatataaa tggaagtact gatataaaac tttttaacct actagattct
      601 tataatttgc tttccatttt atgacgattt ttactcaatt gagtgataga atcaaaaaag
      661 ccatctcaaa aattaatcaa gcaaacaaca ttccaaacaa tgctcgcaaa tcaccaatgt
      721 atcactctcc aattacgtaa ctatgattta atttaagcat agttattgag gttttgtgat
      781 atatagtata aaattaatga gaattaaatt taataatgta aaattcatct tcggggtcgg
      841 gtgtaattcc caaccggcag taaataaagc ctgcgacctg ctagtatgta tcatattagt
      901 ggctgatcta gtgagattct agagccgaca gtatagtctg gatgggagaa gatggaggtt
      961 ttttgttgtg caataatcct cctattctta cgagatgaat ggaaggagaa aattgaatat
     1021 gcaacaaaat aaacgtctta tcacaataag tatgttgagc gcgattgcgt ttgtgttaac
     1081 ttttatcaag tttcctatac catttttgcc accatactta actttagatt ttagtgatgt
     1141 accgtcacta ctagctacat ttacgtttgg accagttgcc ggtatcgtag ttgcactggt
     1201 taaaaattta ttgaactact tatttagtat gggcgatcca gttggaccat ttgctaactt
     1261 tttagcaggc gcaagtttct tattaactgc ttacgccatc tataaaaata aacgttcaac
     1321 aaaatctttg attactggat taatcattgc aacaatcgtt atgactatcg tgttgagtat
     1381 tttgaactat ttcgttctat tacctttgta cggtatgata tttaacttag ctgatatcgc
     1441 aaataatctt aaagtaatca ttgtttcagg aattatacca ttcaatatta ttaaaggtat
     1501 cgttatttct attgtattta ttttactata tagaaggctt gcgaatttct tgaaaagaat
     1561 ttaatcaaat taaagcaaaa taatatacac ataataataa aaagcaggtg actatcaata
     1621 aacgatagct tgcctgcttt ttctatagaa catttgtcta aaaaatcaat tattcaaatt
     1681 ttaaagcgtc cccatcaaat gattcgtctg caattttaat agaatctgta gggcatccat
     1741 caattgcatc ttccatatct tcatataatt cctcaggtac ttctgcagta ccttggttat
     1801 cgtcaaggat tacgaaagca ataccttcgt cgtcgtaatc atatatatct ggcgctgctg
     1861 caccgcatgc accacatgca atacaagtat ccatatcaac gattgtatat tttgccaatg
     1921 tcttcgcctc ctttgataaa aatgctaaaa tagtaatgtg actaaaattt tagacagcat
     1981 catttttatt ttcaaattat ccgttttaca gagtgagggt taaatttgca acacattata
     2041 aaaacagcat tacaacaaac atttaactat aaaacaaata aaagtattta caatatctta
     2101 gttggtaaga aatctcacca aacctttttt gacgcttgta gtcaacaaca gttgtcatta
     2161 tatcacagtt taccactatt aaaatatccg tcttttgagc tatttttaga aaaaatcaat
     2221 gaatttaatg ctgaaatgga aatcatgttg catcctagat atacatttga aagcatgggg
     2281 caaacatttc aagcaattca actattagtg caaaccatgt ctaataccaa acaacatgtt
     2341 tttcattttg taccaatctc tcaaaataat aagatacaag aaactgtaaa gattgtctat
     2401 aactacatta aagaaaacca attaca
//
LOCUS       DAKTIO010000253         2403 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.260, whole genome shotgun sequence.
ACCESSION   DAKTIO010000253 DAKTIO010000000
VERSION     DAKTIO010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.260"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            325..558
                     /locus_tag="O0293_002691"
     CDS             325..558
                     /locus_tag="O0293_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281515.1"
                     /translation="MVFNDWEINVVYSGEHEVVTNEKSLFVIDEFYDVAIAIYYLDGK
                     LKVAHVNYGSDFTIDVANKVFELNIQNPNVDEH"
     gene            1180..1365
                     /locus_tag="O0293_002692"
     CDS             1180..1365
                     /locus_tag="O0293_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281516.1"
                     /translation="MNKHINKKAAKLLEDYKKVQQRKSKETDRGCLKSVIMLIVLFFL
                     IIFGIVACSTNVNFFFQ"
     gene            complement(2018..2368)
                     /gene="scb"
                     /locus_tag="O0293_002693"
     CDS             complement(2018..2368)
                     /gene="scb"
                     /locus_tag="O0293_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-B"
                     /protein_id="HCY0281517.1"
                     /translation="MKFKKYILTGTLALLLSSTGIATIEGNKADASSLDKYLTESQFH
                     DKRIAEELRTLLNKSNVYALAAGSLNPYYKRTIMMNEYRAKAALKKNDFVSMADAKVA
                     LEKIYKEIDEIINR"
BASE COUNT          849 a          325 c          329 g          900 t
ORIGIN      
        1 gacttaaaaa ttagactaaa catgacacta tatgaggtaa tttttaaaat aagaaatttg
       61 aattccatgt tgtcaaaccc tttatataaa agttttgaaa ttgttcaaaa ctttttgaac
      121 acattatgtg ttaatatatt tgcagaatta ttttaaaatc gttttaaaac tatactgaaa
      181 taagaaattt aaaagtataa ttagtacata taattgaaaa agatttctag aaatacaatt
      241 aatgttcatc tagatattgt acgaagttgt atgaataagt taaacttaag ttgaatcaat
      301 ttaaatgttc aaggaggtta acaaatggtt tttaatgatt gggaaattaa cgttgtatac
      361 tcaggagagc atgaggttgt aacaaacgaa aagagtttat ttgtcattga cgaattttac
      421 gatgttgcaa tcgcaatcta ttatttagat ggaaaattaa aagtagctca tgtcaactat
      481 ggttctgatt ttacaattga cgtagctaat aaagtttttg aattgaatat tcaaaatcca
      541 aatgttgatg agcattaaat attaaaaagc atttagaatc ataaagagca tactatatat
      601 agccttttac atgtaaagca aatgaaaatt agataatcta attatttcaa cattaaacaa
      661 tatatcatta taaagaagtt tttaaaatag aagtataagg tggtgaacaa aaattaacat
      721 tgtgtataac attatagtat atatagcatg catgattaat ttcggattaa ttatagtccc
      781 attattaaca acaaatgatg cggattttgt atggacttct tcatcattaa tatatatgat
      841 tcttcttacg tcgattatcc taatagttta tattaaaact aaagatagct tacagttaac
      901 gatatttata cttaatatag ccttaattat tctatactgt gcaccattac tattcattta
      961 tgtattctag tgcttcatta atattttaat gcatcagttt aagtaaattc catttggaaa
     1021 ataaaaagca aatatcatca tcaaattaac atatttaacg ttatactttc ataaaatttg
     1081 aaacaattga acatccttac tttagcttta tatctttatt tcaagtcatg ttttcaacta
     1141 ttttaatgaa ttaaacatat attataagga gtcaacatca tgaataaaca tataaataaa
     1201 aaagccgcaa aactacttga agattataaa aaggttcaac aaagaaaatc gaaagaaact
     1261 gatagaggtt gtttgaaaag tgtcatcatg cttatcgtct tatttttctt aattattttt
     1321 ggtattgtgg catgttctac aaacgtaaac tttttcttcc agtaaaagag tgagatagaa
     1381 atgataaaga accactaatg atttattatg tagtggttct ttatcattag ctgtagctaa
     1441 tgtgtactta aaaaatgaat acatgagtaa agctcatgca taagaaatac taattgctaa
     1501 agaaaaagta tttctttatc gatgtcctac cccaacttgc attgtctgta gaaattgggg
     1561 ttccaatttc tctatgttgg ggccccgcca acttgcacat ttttgtaagc tgacttttcg
     1621 ccagcttcta tattggggcc cccgccaact tgcattgttt gttggaattg gtgatcctat
     1681 ttctctctgt tgtggcccca cgagtattga aaaaacttgt tataagctta ttttcattta
     1741 gtcaactact accaatatag cactagaact ggaaatgata atttaatatt gtacactttt
     1801 gattgtttaa acatgtagat attttaaaaa ataggagagc aaagtaaata attgatatag
     1861 ttattttgag agtaatccta ggaactattg tatttatatt tgtctcccct acttttaaat
     1921 gtcattcatt atacataagc attttgatat agaatttatc acatatgcaa attgaaaaca
     1981 ggttaagacc attttttgtc tcaacctgtt ttatttatta tctatttata atttcatcaa
     2041 tttctttgta tattttttct aatgcaactt tagcatcagc cattgatacg aaatcatttt
     2101 tcttaagtgc cgctttagct ctatattcat tcatcataat cgtacgttta taatatggat
     2161 ttaagcttcc tgcagctaat gcatatacat tcgatttgtt aagtaaagtt cttaattctt
     2221 ctgctatgcg tttatcatga aactgacttt cagttaaata tttgtccaga ctacttgcat
     2281 ctgctttatt cccttctata gttgctatcc cagttgatga taaaagtaat gctaatgttc
     2341 ctgttaatat atatttttta aatttcatta taagctctcc ttaaaattga tacttactga
     2401 gta
//
LOCUS       DAKTIO010000254         2337 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.262, whole genome shotgun sequence.
ACCESSION   DAKTIO010000254 DAKTIO010000000
VERSION     DAKTIO010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.262"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            6..398
                     /locus_tag="O0293_002694"
     CDS             6..398
                     /locus_tag="O0293_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0281518.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            399..1838
                     /locus_tag="O0293_002695"
                     /note="aph(2'')-Ia"
     CDS             399..1838
                     /locus_tag="O0293_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HCY0281519.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(1968..>2337)
                     /locus_tag="O0293_002696"
     CDS             complement(1968..>2337)
                     /locus_tag="O0293_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCY0281520.1"
                     /translation="KNSKSFREAVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACA
                     SLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAV
                     LMDLHDEWIYSSRKYINFDK"
BASE COUNT          923 a          210 c          401 g          803 t
ORIGIN      
        1 agtttatgga agaagtttta attgatgata atatggtttt tgatattgat aatttaaaag
       61 gatttcttaa tgataccagt tcatttgggt ttatagctaa agaaaataat aaaattatag
      121 gatttgcata ttgctataca cttttaagac ctgatggaaa aacaatgttt tatttacact
      181 caataggaat gttacctaac tatcaagaca aaggttatgg ttcaaaatta ttatctttta
      241 ttaaggaata ttctaaagag attggttgtt ctgaaatgtt tttaataact gataaaggta
      301 atcctagagc ttgccatgta tatgaaaaat taggtggtaa aaatgattat aaagatgaaa
      361 tagtatatgt atatgattat gaaaaaggtg ataaataaat gaatatagtt gaaaatgaaa
      421 tatgtataag aactttaata gatgatgatt ttcctttgat gttaaaatgg ttaactgatg
      481 aaagagtatt agaattttat ggtggtagag ataaaaaata tacattagaa tcattaaaaa
      541 aacattatac agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg
      601 ttcctattgg atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt
      661 atccaaaaac tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt
      721 ggagtaaagg aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa
      781 gaaatgctaa tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat
      841 accaaaaatc tggttttaga attattgaag atttgccaga acatgaatta cacgagggca
      901 aaaaagaaga ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg
      961 caatgaaata tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa
     1021 tcggtagtgg ttatgatagt gtggcatatt tagttaataa tgaatacatt tttaaaacaa
     1081 aatttagtac taataagaaa aaaggttatg caaaagaaaa agcaatatat aattttttaa
     1141 atacaaattt agaaactaat gtaaaaattc ctaatattga atattcgtat attagtgatg
     1201 aattatctat actaggttat aaagaaatta aaggaacttt tttaacacca gaaatttatt
     1261 ctactatgtc agaagaagaa caaaatttgt taaaacgaga tattgccagt tttttaagac
     1321 aaatgcacgg tttagattat acagatatta gtgaatgtac tattgataat aaacaaaatg
     1381 tattagaaga gtatatattg ttgcgtgaaa ctatttataa tgatttaact gatatagaaa
     1441 aagattatat agaaagtttt atggaaagac taaatgcaac aacagttttt gagggtaaaa
     1501 agtgtttatg ccataatgat tttagttgta atcatctatt gttagatggc aataatagat
     1561 taactggaat aattgatttt ggagattctg gaattataga tgaatattgt gattttatat
     1621 acttacttga agatagtgaa gaagaaatag gaacaaattt tggagaagat atattaagaa
     1681 tgtatggaaa tatagatatt gagaaagcaa aagaatatca agatatagtt gaagaatatt
     1741 atcctattga aactattgtt tatggaatta aaaatattaa acaggaattt atcgaaaatg
     1801 gtagaaaaga aatttataaa aggacttata aagattgatt atataatata tgaaaagcta
     1861 ttataaaaga cattagtatt aaatagttta aaaaaatgaa aaataataaa ggaagtgagt
     1921 caagtccaga ctcctgtgta aaatgctata caatgttttt accatttcta cttatcaaaa
     1981 ttgatgtatt ttcttgaaga ataaatccat tcatcatgta ggtccataag aacggctcca
     2041 attaagcgat tggctgatgt ttgattgggg aagatgcgaa taatcttttc tcttctgcgt
     2101 acttcttgat tcagtcgttc aattagattg gtactcttta gtcgattgtg ggaatttcct
     2161 tgtacggtat attgaaaggc gtcttcgaat ccatcatcca atgatgcgca agcttttgaa
     2221 tattttggtt gatcgatata atcatgaatc aatcgatttt tagcctcacg cgctaagtta
     2281 atatctgtga acttaaaaat tcctttaaca gcttctctga aagattttga atttttt
//
LOCUS       DAKTIO010000255         2320 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.263, whole genome shotgun sequence.
ACCESSION   DAKTIO010000255 DAKTIO010000000
VERSION     DAKTIO010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.263"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            158..1147
                     /gene="trpS"
                     /locus_tag="O0293_002697"
     CDS             158..1147
                     /gene="trpS"
                     /locus_tag="O0293_002697"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCY0281521.1"
                     /translation="METLFSGIQPSGIPTIGNYIGALKQFVDVQNDYDCYFCIVDQHA
                     ITMPQDRLKLRKQTRQLAAIYLASGIDPDKATLFIQSEVPAHVQAGWMLTTIASVGEL
                     ERMTQYKDKAQKAVEGIPAGLLTYPPLMAADIVLYNTNIVPVGDDQKQHIELTRNLVD
                     RFNSRYNDVLVKPEIRMPKVGGRVMSLQDPTRKMSKSDDNAKNFISLLDEPNVAAKKI
                     KSAVTDSDGIIKFDRDNKPGITNLISIYAGLTDMPIKDIEAKYEGEGYGKFKGDLAEI
                     VKAFLVEFQEKYESFYNSDKLDDILDQGRDKAHKVSFKTVKKMEKAMGLGRKR"
     gene            complement(1189..2070)
                     /locus_tag="O0293_002698"
     CDS             complement(1189..2070)
                     /locus_tag="O0293_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0281522.1"
                     /translation="MQNKSKSPFKIAFSKFIHNKIAMLSVIFLLIITIVSIIAPLIAP
                     FPVNQQDLLNIKGEMTAQNILGTDSGGRDNFSRLLYAGRISLSIGITSTIGMLLIGIT
                     VGVISGYFGGIVDTLLMRITEFVMLFPFLIFAIVLNAALGDKIKNPYGSAIILVLVII
                     VLSWGGIARLVRGKVLQEKENEYFLAAKSIGTPTYKIILKHLLPNILSVVIVQATLLF
                     AGMIVVESGLSFLGFGISKAIPSWGNMLSDAQEGDVISGKPWIWMPPAIMITLTILSI
                     NFVGEGIKDAFNPRGRR"
     gene            complement(2082..>2320)
                     /locus_tag="O0293_002699"
     CDS             complement(2082..>2320)
                     /locus_tag="O0293_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCY0281523.1"
                     /translation="IPIITFLGADIVSILGGAVITETIFSYNGIGKLFLESVIGQDYP
                     LMMALTLFFSFLGLLGNLISDITYGFIDPRIRSN"
BASE COUNT          856 a          391 c          350 g          723 t
ORIGIN      
        1 aacctaattt ttcagataag tttctacact tataaaaaag attattaatc cctttgttag
       61 tagtaagtta tacgtatatt ctaacacatc ttacattttt aagaaatact gttataatga
      121 taattattaa aatattacta agaaagtagg catttaaatg gagacattat tttcaggcat
      181 ccaacctagt ggaattccta ctattggaaa ttatattggc gcactaaaac aatttgttga
      241 tgtgcaaaat gactatgatt gttatttctg tatcgtagat caacatgcaa ttacaatgcc
      301 acaagatcgt ttaaaattac gtaaacagac cagacaatta gcagcgattt atttagcttc
      361 tggtatagat ccagacaaag caacattgtt catacaatct gaagtccctg cacacgtaca
      421 agcaggatgg atgttaacta cgattgcttc tgttggagaa ttagagcgta tgacgcaata
      481 caaagataaa gctcagaaag cagttgaagg tatacctgct ggtctattaa catatccacc
      541 tttaatggca gctgatattg ttctttacaa tactaatatc gttccagttg gagatgacca
      601 aaagcagcat atcgaattga ctcgtaacct tgtagataga tttaatagtc gctataatga
      661 tgtgcttgtg aaacctgaaa ttcgtatgcc taaagttggt ggacgtgtca tgagtttaca
      721 agacccaaca agaaaaatga gtaagagtga tgataatgct aaaaacttca tttcattatt
      781 agacgagccg aatgttgcag ctaaaaaaat taaaagcgca gtaactgatt cagatggtat
      841 tattaaattt gatcgtgaca acaagccagg tataacaaat ttaatttcaa tatacgctgg
      901 attaacagac atgccaatta aagatattga ggcaaaatat gagggcgaag gttatggtaa
      961 atttaaaggt gaccttgctg aaatagttaa agcattttta gtagaattcc aagaaaaata
     1021 cgaaagtttc tataactcag ataaacttga tgatatttta gatcaaggta gagataaagc
     1081 acacaaagtt tcatttaaaa ctgtcaaaaa aatggaaaaa gcgatgggtt taggacgtaa
     1141 gagataaaat aaagaggtac tagaattaac tagtgcctct tttattattt aacgtctacc
     1201 tctaggatta aaagcatctt taatcccttc ccctacaaag tttatactta atatagttaa
     1261 tgtaatcatt atagcaggtg gcatccatat ccacggttta ccacttataa catccccttc
     1321 ttgagcatca ctcaacatat taccccaaga tggtattgct ttactaattc cgaatcctaa
     1381 aaagctcaat cctgattcca ctacaatcat accggcaaat aacaatgttg cttgtacgat
     1441 aactacactt aatatattcg gcaaaagatg tttcaaaata attttatatg tgggtgtacc
     1501 aattgatttt gctgccaaaa agtattcatt ttctttttct tgaagtactt taccacgaac
     1561 aagtcttgca atacctcccc aacttaatac gataataact agaacaagaa ttatggcaga
     1621 tccataagga tttttaattt tatctccaag tgcagcattt aatacaattg caaatattaa
     1681 aaatggaaat aacataacaa attcggttat tctcattaat aatgtatcaa caatacctcc
     1741 aaaataacca gaaatcactc caactgtaat tccaatcaaa agcattccta ttgtagatgt
     1801 aattccaatg gataatgaaa tacgacctgc atataacaaa cgactaaagt tatccctacc
     1861 accagagtct gtaccaagaa tgttttgtgc tgtcatttca ccttttatat ttaataaatc
     1921 ttgttggttc actggaaaag gagctattaa tggcgctata attgatacaa tagttatgat
     1981 taataaaaaa ataaccgata acattgcaat tttattatga ataaatttag aaaatgcaat
     2041 tttaaaaggc gattttgact tattttgcat tctaatcccc cctagttact tctaattctt
     2101 ggatctataa atccataagt aatatcagaa atcaaattac ccagtaaacc taaaaatgag
     2161 aaaaataacg ttaatgccat cattaatgga tagtcttggc ctattaccga ttctaaaaat
     2221 aatttaccga taccgttata tgaaaagata gtctccgtaa tcacggctcc acctaaaata
     2281 cttacaatat cagcacctaa aaatgtaata ataggtatta
//
LOCUS       DAKTIO010000256         2311 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.264, whole genome shotgun sequence.
ACCESSION   DAKTIO010000256 DAKTIO010000000
VERSION     DAKTIO010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.264"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            484..798
                     /locus_tag="O0293_002700"
     CDS             484..798
                     /locus_tag="O0293_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCY0281524.1"
                     /translation="MTYKELATFLKVLSDSSRLEILDLLSCGELCACDLLAHFQFSQP
                     TLSYHMKALVKTNLVTTRKIGNKHLYQLNHNIFESVINNLSKVHTSNQRCICHNLKTG
                     EC"
     gene            798..2090
                     /gene="arsB"
                     /locus_tag="O0293_002701"
     CDS             798..2090
                     /gene="arsB"
                     /locus_tag="O0293_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter membrane subunit
                     ArsB"
                     /protein_id="HCY0281525.1"
                     /translation="MMTTLATLIFLVTLLFVLWQPKGLDIGITALTGAFIAVITGVVS
                     FSDVFEVTGIVWNATLTFVSVILISLILDKVGLFEWSAIHMLHASKGNGLKMFVYIIL
                     LGAIVAAFFANDGAALILTPIVLAMVKNIGFSKRAIFPFIIASGFIADTTSLPLIVSN
                     LVNIISADYFHVGFVRYFSRMIIPNLFSLLASIIVLWLYFRKAIPKTFDDNNIKHPKD
                     AINDLKLFKISWIVLVILLFGYLISEFTKIPVSIFTGIIAFIFLMLARKSNAVNIKQV
                     IKGAPWNIVLFSIGMYIVVFGLRNAGITLILAKILEYISNYGLFSTILGMGFISAFLS
                     SIMNNMPTVLIDAIAIGQSNVHGMLKEGLIYANVIGSDLGPKITPIGSLATLLWLHVL
                     TQKDVKISWGTYFKTGIIITIPVLFITLIGLYLTLIIF"
     gene            2108..>2311
                     /locus_tag="O0293_002702"
     CDS             2108..>2311
                     /locus_tag="O0293_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HCY0281526.1"
                     /translation="MTKKTIYFICTGNSCRSQMAEGWAKQILADDWNVYSAGIETHGV
                     NPKAIEAMKEVGIDISNHTSDLID"
BASE COUNT          756 a          349 c          359 g          847 t
ORIGIN      
        1 aactattacc tataattata gcaatttggt atttccaata aaatttatta aggtaagcta
       61 ttatttcact ttcacaacca ttaaaccatt taaaactttt catttaatat caaccttagt
      121 gtttgtgtta aaatattttt aacgaggtga attaaattga aatttatagc aaagatatta
      181 atctcattag taattctatt tatgattttt agtcgtaaaa acaaagcttc ttaatcaata
      241 cccttgatta caaaataaat tcaactaagg tgattggcca tacactcaag atatggctaa
      301 tcaccttttt caatgcagaa caaagagtat attttgcaaa atcaatatct ataatttatc
      361 tttaataccc ttttaatact aaaaccaaat ttaaattaca tagtcatatc gtgtaatata
      421 ccaaaggaac atagacattc atctatatag atgataatct atttaacgtc taggaggtat
      481 gttatgacgt ataaagaact agcaacattt ttaaaagttt tatcagattc aagcagatta
      541 gaaatactag atttactttc ttgtggagag ttatgcgctt gtgatttgtt agcacatttt
      601 caattctctc aacctacact tagctatcat atgaaagcat tagtaaaaac caacttagtt
      661 acgacacgaa aaatcggaaa taaacattta taccagctca atcataatat ttttgagtcc
      721 gtaattaata acttgtctaa ggttcatacc tctaaccaac gatgtatttg tcataacctt
      781 aagactggtg aatgctaatg atgacaactt tagcgacact catttttcta gtaactttat
      841 tatttgtatt atggcaacct aaaggcctag atattggcat taccgcacta actggtgcct
      901 ttattgctgt tattactggt gttgtaagtt tttccgatgt tttcgaagta acaggtattg
      961 tttggaatgc tactttgact tttgtctcag tcattcttat ttcattaata ttagataaag
     1021 ttggattatt cgaatggtca gctattcaca tgcttcatgc ttcaaaaggc aatggtttaa
     1081 aaatgttcgt ttatatcata ttattgggtg ccattgttgc tgcatttttc gcaaatgatg
     1141 gcgcagcgtt aatcttaacg cctattgtat tagcgatggt taaaaatata ggttttagta
     1201 agcgggccat attccctttt attattgcga gtggttttat agctgacaca acttctttac
     1261 ctttaatcgt gagcaatcta gtgaatatta tatctgctga ttattttcat gtaggattcg
     1321 ttcgatattt tagtagaatg attataccta atttattctc gcttttagca agtattatag
     1381 tattgtggtt atattttaga aaagcgatac ctaaaacgtt tgatgataat aatataaagc
     1441 atcctaaaga tgccattaat gatttaaagc tatttaaaat ttcatggatt gttctagtta
     1501 tattactttt cggctatcta atcagtgaat ttactaaaat tccggtatca attttcactg
     1561 gaatcattgc ttttattttt ctaatgttgg ctcgtaaatc aaatgctgta aatattaagc
     1621 aagtcattaa gggcgcacct tggaatatag tattattttc aattggtatg tatatcgtcg
     1681 tattcggctt aagaaatgct ggcattactt taatattggc taaaatatta gaatatattt
     1741 ccaattacgg tctatttagc actattttgg gaatgggctt catttcagcg tttttatcat
     1801 caataatgaa taatatgcct acagttttaa tagatgcgat tgctattggt caatcaaatg
     1861 tccatggcat gttaaaagaa ggcctaattt atgcgaatgt tatcggttct gatttaggtc
     1921 caaaaattac accgataggc tctttagcta cattactgtg gttacacgtc ttaacacaaa
     1981 aagatgttaa gatttcttgg ggcacatact ttaaaactgg tatcatcatt acaattccag
     2041 tactatttat aaccctcata gggttgtatc taacacttat catattttaa ttaattagga
     2101 gattgttatg actaaaaaaa caatttattt tatatgtaca ggcaactcat gtcgaagtca
     2161 aatggctgaa ggttgggcta aacaaatctt agcggatgat tggaatgtat attctgctgg
     2221 tatcgaaaca cacggtgtta atcccaaagc gatagaagct atgaaagaag taggcattga
     2281 tatatcaaat catacatcag atttaatcga t
//
LOCUS       DAKTIO010000257         2310 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.265, whole genome shotgun sequence.
ACCESSION   DAKTIO010000257 DAKTIO010000000
VERSION     DAKTIO010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.265"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..1142
                     /locus_tag="O0293_002703"
     CDS             <1..1142
                     /locus_tag="O0293_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HCY0281527.1"
                     /translation="MIKGKQHFKTIRFINTVCKSILLVLIANVMIENTSVIKDLNKIK
                     ETEKYWNVLDDYYTIEFAPYHETKQSLIDNMVRSEQLVKASEAENNAILFKPKGDSVD
                     NDNFSPDEGNVILVNNQFWSIYYKQFQPDIPIKNQKNNVEVIIPQKFHAMRNEINQAY
                     HSWFEFVQNKNNKENKLSIQFINKNDCRIFSFDARDSRHLSFIEAPIIVNVQASDLSN
                     DFYYAMISQGGYLFKNYDALVKNIEKYHLDGEISGITNYKDSVMEMYHENNLKLTVLN
                     FSQIIIAIILIIIILFDVKYYFEQHRKLLVIKKLYGYSTLRANYQYLLINNIVVIFIG
                     ILTNVILHSHYIMMLFATILVVQILLQICSLYYHGRRFNEVIKEF"
     gene            1145..1465
                     /locus_tag="O0293_002704"
     CDS             1145..1465
                     /locus_tag="O0293_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCY0281528.1"
                     /translation="MKFIIAILLGLILSITIAFTIIHHPILDRFNPFLKTEYSYAKVP
                     KGTQQYVNITAYSERGEKLDYKLTFNGFSPSRTYVEIKHKGQYVISITYVEKEDTPKE
                     VRQE"
     gene            1462..2103
                     /locus_tag="O0293_002705"
     CDS             1462..2103
                     /locus_tag="O0293_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0281529.1"
                     /translation="MIELKDLTIQKGNTHILKKLNLKFQCGKSYALIGKSGCGKSTLL
                     NTIAGLEKTGKQYVYFNGQLEQFKSNFYRDKLGYLFQNYGLIDNLTVNENLDIGLAYK
                     KISKKEKEQIKIRYIEQFGLSNSLKRKVHTLSGGEQQRVALIRMMLKDPIVMLADEPT
                     GALDPKTGQMIIQSLFGLVDENKVLILATHDMAIANQCDEIIDLEQYRKVASM"
BASE COUNT          915 a          280 c          374 g          741 t
ORIGIN      
        1 atatgattaa aggtaagcag cactttaaaa caattcgttt tattaataca gtttgtaaaa
       61 gtattctctt agtactaata gctaatgtaa tgatagaaaa tactagtgtt attaaagatt
      121 tgaataaaat aaaagaaact gagaaatatt ggaatgtatt agatgattat tacacgattg
      181 aatttgcacc ttatcacgaa acaaaacaaa gtttgattga taatatggtg cgatcagaac
      241 aattagtaaa ggctagtgaa gcagaaaata atgcgatttt attcaaacca aagggtgact
      301 ccgttgacaa tgacaacttt tcgcctgatg aggggaatgt aatattagta aataatcagt
      361 tctggtcgat ttattacaag cagtttcaac ctgatattcc gataaaaaat caaaaaaata
      421 atgtcgaagt aattattcca caaaagtttc atgcaatgcg taatgaaatc aatcaagcat
      481 atcattcatg gtttgaattt gtacaaaata aaaataataa agagaataag ttatctatac
      541 agtttatcaa caaaaatgat tgtcgaattt tttcatttga tgcacgagat agtcgccatt
      601 tgtcatttat agaggcgcca atcattgtga atgttcaggc atcagattta tcgaatgatt
      661 tttattatgc catgatcagt caaggcgggt atttattcaa aaattatgac gcgctagtaa
      721 aaaatattga aaagtatcat cttgatgggg aaatcagtgg aataaccaat tataaagata
      781 gcgtgatgga aatgtatcat gaaaacaatt tgaaattaac agtactcaac ttttcacaaa
      841 tcattatcgc aatcatttta ataattatta ttttatttga tgtgaaatat tattttgaac
      901 agcatcgaaa attactcgta atcaaaaagc tatatggtta ttcaacatta agagccaatt
      961 accaatactt attaataaat aatatagttg ttatttttat tggaatattg acgaatgtaa
     1021 ttttacattc tcactatata atgatgttat ttgcaacgat tcttgtcgtt caaatattat
     1081 tgcagatttg cagcctatac tatcatggcc gacgttttaa tgaagttatc aaggagtttt
     1141 aactatgaaa tttatcattg caatattatt aggattaata ttatccatta ctattgcttt
     1201 tacaatcata catcatccta tacttgatcg ttttaatcct ttcttaaaaa cggagtatag
     1261 ttatgccaaa gtgccaaaag gtacgcaaca atatgttaat attacggctt atagtgaaag
     1321 aggggaaaag cttgattata aattaacatt taatggattt tcacctagta gaacgtatgt
     1381 cgaaataaag cataaagggc aatatgtcat atcgatcaca tatgttgaaa aagaggatac
     1441 accaaaagag gtaagacaag aatgatagaa ttaaaagatt taaccataca aaaaggtaat
     1501 acacatattt tgaagaaact taatttgaaa tttcaatgtg gaaaatcata tgcacttatt
     1561 ggtaaaagtg gatgcggtaa atctacatta ttaaatacta ttgctggact tgaaaaaaca
     1621 ggaaaacaat atgtctattt taatggtcaa ttagaacaat ttaaatctaa tttttacaga
     1681 gataaattag gatatttatt tcaaaattat ggattaatcg ataatttgac agtaaatgaa
     1741 aatttagata ttggattagc atataaaaaa ataagtaaga aagaaaaaga acaaataaag
     1801 atacgttata tagaacagtt tggtctgtca aacagtttaa aaagaaaagt tcatacgcta
     1861 agtggaggtg aacaacaacg tgtcgcttta attagaatga tgttaaaaga tccgattgtt
     1921 atgttagctg atgaaccaac gggtgcgtta gatcctaaaa caggacagat gattattcaa
     1981 tcattatttg gtttggtcga tgaaaataaa gtgctgattt tagcaacaca tgatatggct
     2041 attgcaaatc aatgtgacga aataatagat ttagaacagt atagaaaagt agcatctatg
     2101 tgatatccaa aagataaaat taaaagccaa tcaatgggaa atgttgactt caacactccg
     2161 tttgattggc ttttatactg tgttactaag ttatgaaagt aacaacggca aactgaaaaa
     2221 aagcaatata ataatattta atacgattag taataaatgt aattgatttt tcgttttaat
     2281 atagcaaaaa acaatgacta aagataacaa
//
LOCUS       DAKTIO010000258         2237 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.266, whole genome shotgun sequence.
ACCESSION   DAKTIO010000258 DAKTIO010000000
VERSION     DAKTIO010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.266"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            181..543
                     /locus_tag="O0293_002706"
     CDS             181..543
                     /locus_tag="O0293_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YojF family protein"
                     /protein_id="HCY0281530.1"
                     /translation="MLEPIKEQEVIDLLASFEHKPVYLHVETTNGAYANHFDQRVFNA
                     GTFLRNIQITYTHAQLKGGNKEPYRIGLKLSNGGWVYVQGLTHFEVNEHDEFLIAGFN
                     YEGQLAAALQISERPFNL"
     gene            557..1222
                     /gene="bshB2"
                     /locus_tag="O0293_002707"
     CDS             557..1222
                     /gene="bshB2"
                     /locus_tag="O0293_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB2"
                     /protein_id="HCY0281531.1"
                     /translation="MTDERHVLVIFPHPDDETFSSAGTLANYIQKGIPVTYACLTLGQ
                     MGRNLGNPPFATRESLPSIRERELEEACKVIGITDLRKMGLRDKTVEFEPYEHIDGMI
                     KSLIDDTNPSLIISFYPGYAVHPDHEATADAVIRTVERMPKEERPRLTLVAFSNDATE
                     ALGEPDIQNDITDFKELKIKAFEAHASQTGPFLKQLASPEIDGKQHSFLTVEPYWTYH
                     FKS"
     gene            1235..2113
                     /locus_tag="O0293_002708"
     CDS             1235..2113
                     /locus_tag="O0293_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HCY0281532.1"
                     /translation="MTEFDLSTREGRWKHFGSVDPIEGTKPTTKNEMTDLQSTHKDFL
                     FEIEEVGIKNLVYPVLVDQYQTAGTFSFSTSLTKDEKGINMSRIIESVEKHYDNGIEL
                     EFNTLYQVLRTLQTNMKQNAAGVDVSGKWFFDRYSPTTNIKAVGNADVTYGLAIDGDK
                     VTRKELTIEATVTTLCPCSKEISEYSAHNQRGVVTVKTYINKDQDIVDDYKNKILDAM
                     EANASSILYPILKRPDEKRVTERAYENPRFVEDLIRLIAADLVEFDWLDGFDIECRNE
                     ESIHQHDAFAKLKYRK"
BASE COUNT          790 a          365 c          401 g          681 t
ORIGIN      
        1 tcaaattcca acaaattttt gatttttaaa agtaaaaaac taatgctgta tgattacgta
       61 atcctgagta attacccttc atgaaaatta atttataaat aattgagcaa tcgtatacaa
      121 ttatcgaata gtttttagta aaatagaaag ataaacacat tgatagtgta aggagtgaac
      181 gtgttggaac caattaaaga acaagaagtt atagatttac ttgcttcttt tgaacataaa
      241 cctgtgtatc tgcatgttga aacgacaaat ggggcctatg cgaaccattt cgatcaacgt
      301 gtttttaatg ctggtacttt tttaagaaac atacaaataa catatacgca tgcacaacta
      361 aaaggtggta ataaagaacc ttatcgcatt ggtcttaaac tatcaaatgg tggttgggtt
      421 tatgtacaag gtctaacgca ttttgaggtc aatgagcatg atgaattttt aattgcaggt
      481 tttaattacg aaggtcaact cgctgcagca cttcaaatta gtgagcgacc atttaattta
      541 tagaggaggc atcaacatga ctgacgaaag acatgtatta gtaatatttc cacatcctga
      601 tgatgaaacc ttctcatctg caggcacatt agcaaattat atccaaaaag gcatacctgt
      661 aacatatgca tgtttaaccc taggacaaat gggcagaaat ttaggtaatc ccccatttgc
      721 aacacgcgaa tcattaccaa gtattcgtga gcgtgaacta gaagaagctt gtaaagttat
      781 cggtattact gatttaagaa aaatgggttt acgtgacaaa actgtcgaat ttgaacccta
      841 tgaacatata gatggtatga ttaaatcttt aatcgatgat acaaatccat cattgattat
      901 ttcattttat cctggatatg cagtacatcc tgatcacgaa gcaacagcag acgctgtaat
      961 tcgaacggtt gaacgcatgc ctaaagaaga acgccctcgt ttaactttag ttgcgttcag
     1021 taatgatgca accgaagctc ttggagaacc tgatattcaa aatgatatta cagattttaa
     1081 agaacttaaa attaaggcat ttgaagcaca tgcttctcaa acaggtccat ttttaaaaca
     1141 attagcaagt cctgaaatcg atggtaaaca acacagcttt ttaacagtcg aaccatattg
     1201 gacatatcac tttaaatctt aacggaggaa aaaaatgact gaatttgatt tatcgacacg
     1261 agaaggtcgt tggaaacact ttggttccgt agatcctatt gaaggcacga aacctactac
     1321 taagaacgaa atgacagatt tacaaagtac acataaagat tttttatttg aaatagaaga
     1381 agttggtatt aaaaacttag tgtaccctgt acttgttgac cagtatcaaa cagctggcac
     1441 atttagtttt tcaacaagtt taacaaaaga cgaaaaaggt attaatatga gccgtataat
     1501 cgaaagtgtt gaaaagcact atgataatgg tattgaactt gaatttaata cactttatca
     1561 ggtgttacgc actttacaaa cgaatatgaa acaaaatgcg gcaggcgttg atgtttctgg
     1621 gaaatggttc tttgatcgct atagtccaac cacaaatatt aaagcagttg gtaatgccga
     1681 tgtcacttat ggattagcaa ttgacggtga taaagttaca cgtaaagaac ttactattga
     1741 agcaactgtc acaacgctat gcccttgttc aaaagaaata agtgagtatt cagcgcacaa
     1801 ccaacgtggt gttgtcactg ttaaaacata tattaataaa gatcaagaca tagttgatga
     1861 ctataaaaat aaaattttag atgcaatgga agctaatgca agttcaattc tataccctat
     1921 tttaaaacgt cctgatgaaa aacgtgtgac agagcgtgca tatgaaaatc cacgttttgt
     1981 cgaagactta attcgtttaa ttgccgcgga cttagttgaa tttgattggt tagacggttt
     2041 cgatatcgaa tgtcgtaatg aagagtctat ccatcaacat gatgctttcg ctaaattaaa
     2101 gtatcgcaaa taatatcata tgattaattc aaacgcgtaa cattagttgc gcgtttttat
     2161 tataccttct agagttgtat atctctttta taaatcttct catttgatgt gcctgagtca
     2221 taaatcaata tcctaag
//
LOCUS       DAKTIO010000259         2232 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.267, whole genome shotgun sequence.
ACCESSION   DAKTIO010000259 DAKTIO010000000
VERSION     DAKTIO010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.267"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            288..1490
                     /locus_tag="O0293_002709"
     CDS             288..1490
                     /locus_tag="O0293_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCY0281533.1"
                     /translation="MCNDTLELLRIKDENIKYINQEIDVIIKGKKATVVNAVLTYKPS
                     ACYCCGVKNEGQIHKHGKRVSRITLLKTQGYNTYLNLAKQRFKCLECNGTFTAKTSIV
                     DESCFISRCVTQKVIEEATKVKTEIDTAEDNCISPSTVSRIRTKAANSLRIKPFNCLP
                     EHIAMDEFKSVKNVTGSMSFIFIDNDTHDVIDILENRTTRFLRAYFERFDLKNRQQVK
                     TVTIDMYEPYVRLFRDLFPNAAIIFDRFHIVQHLNRELNKYRVQVMNEYRNKKGPDYT
                     IFKNNWKVLLMDTSKTIFSKYRWNKSFKAYKRSSDIVEFMLSKDDILRHSYELVQGLR
                     KDLRLCNWPKFINRLNSVSKKSVSKGVWKVVKYYRKHQRMLRNTIYYPAFNNGAIEGI
                     NNKIKLIK"
     gene            1626..1730
                     /locus_tag="O0293_002710"
     CDS             1626..1730
                     /locus_tag="O0293_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281534.1"
                     /translation="MYFKNSSLNYIPTTFGGEPKKKHFPKMESASSEP"
     gene            complement(1721..1793)
                     /locus_tag="O0293_002711"
     tRNA            complement(1721..1793)
                     /locus_tag="O0293_002711"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1758..1760),aa:Lys,seq:ttt)
     gene            complement(1797..1871)
                     /locus_tag="O0293_002712"
     tRNA            complement(1797..1871)
                     /locus_tag="O0293_002712"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1837..1839),aa:Gln,seq:ttg)
     gene            complement(1881..1964)
                     /locus_tag="O0293_002713"
     tRNA            complement(1881..1964)
                     /locus_tag="O0293_002713"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1928..1930),aa:Tyr,seq:gta)
     gene            complement(1994..2069)
                     /locus_tag="O0293_002714"
     tRNA            complement(1994..2069)
                     /locus_tag="O0293_002714"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2034..2036),aa:Val,seq:tac)
     gene            complement(2077..2148)
                     /locus_tag="O0293_002715"
     tRNA            complement(2077..2148)
                     /locus_tag="O0293_002715"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2113..2115),aa:Glu,seq:ttc)
     gene            complement(2150..2224)
                     /locus_tag="O0293_002716"
     tRNA            complement(2150..2224)
                     /locus_tag="O0293_002716"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2190..2192),aa:Asn,seq:gtt)
BASE COUNT          807 a          386 c          399 g          640 t
ORIGIN      
        1 ttaaaatagc catattacta ttaagataga taaattgaat cgacttggta taaagcacaa
       61 taaaatagca cttttacaaa tatattatta gaaaagttga aacaaatata taaccccaga
      121 taatataatc attatcatta atgtaatacc aaaaaaagaa gttaaacagg ttcttcatct
      181 tttatggtgg gaaggtaaaa cttcctgctt tttttaatac acaaaaagcg caattgcctc
      241 tataatttaa agtgaccaaa cccaaactaa aggagacaag tgcgcctatg tgtaatgata
      301 ccttagaatt actaagaata aaagatgaaa atataaaata tataaaccaa gaaattgacg
      361 tcattatcaa aggaaaaaaa gcaacagtgg ttaatgctgt actaacgtat aagccttcgg
      421 cctgttattg ttgtggagtt aaaaatgaag gacaaattca taaacatggt aagcgtgttt
      481 ctcgtattac tttacttaaa actcaagggt ataacacata cctcaactta gctaaacaac
      541 gttttaaatg cttagaatgc aatggcactt ttactgctaa aacgtcaatt gttgatgagt
      601 cgtgttttat ctcaagatgt gttactcaaa aagttataga agaagctact aaagttaaaa
      661 cagagattga tactgcagaa gataactgta tctctccatc tactgtaagt cgtattagaa
      721 ctaaagcggc taattcatta cgaattaaac cctttaattg tttgccagaa cacatcgcta
      781 tggatgaatt taaaagcgtt aaaaatgtaa ctggatcaat gagtttcatt tttatagata
      841 atgatactca tgatgttata gatattttag aaaatagaac tacaagattc ttgcgtgcct
      901 atttcgagcg attcgattta aaaaatcgac aacaagttaa gacggttact attgacatgt
      961 atgaacccta tgtccgatta tttcgcgacc tatttcctaa tgcagctatt atttttgaca
     1021 gattccatat cgttcaacat ttaaatagag aacttaataa gtatcgtgta caagttatga
     1081 atgaataccg taataaaaaa ggacctgatt atacaatttt taagaataac tggaaagtcc
     1141 tattgatgga tactagtaaa accatattta gtaaatacag atggaataaa tcttttaagg
     1201 cttataaacg ctcatctgac attgtagaat tcatgctttc aaaagacgat atactacgac
     1261 actcctacga acttgtccaa ggattacgaa aagacctaag gttatgtaat tggcctaaat
     1321 ttattaatcg tttaaattca gttagtaaaa agtctgtgag taagggtgta tggaaagtgg
     1381 ttaaatatta tagaaaacat caaaggatgt taagaaatac aatttattac ccagcattta
     1441 ataatggtgc tatagaagga attaataata agataaaatt aatcaagtga atttcttttg
     1501 gttacagaaa tttcaacaac tttaaagcac gtataatgat gattttcagc ttgtacaaag
     1561 gagaaaaaaa gaagacaacc aagcccaata atggactggc cgcctaataa taaaagctct
     1621 aaaagttgta ttttaaaaat agttctttaa attatatacc caccacattt ggtggagaac
     1681 ctaaaaaaaa gcacttccca aaaatggaaa gtgcaagtag tgagccatag aggattcgaa
     1741 cctctgaccc tctgattaaa agtcagatgc tctaccaact gagctaatgg ctctaatggc
     1801 tgggctagct ggattcgaac caacgagtga cggagtcaaa gtccgttgcc ttaccgcttg
     1861 gctatagccc aatatataga tggtggaggg gggcagattc gaactgccga acccgaagga
     1921 gcggatttac agtccgccgc gtttagccac ttcgctaccc ctccagctta ttcatataat
     1981 ttaataatca aaatggtgga gaatgacggg ttcgaaccgc cgaccctctg cttgtaaggc
     2041 agatgctctc ccagctgagc taattctcca aaataatgac tcctacggga ctcgaacccg
     2101 tgttaccgcc gtgaaagggc ggtgtcttaa ccgcttgacc aaggagccat ggctccacag
     2161 gtaggactcg aacctacgac cgatcggtta acagccgata gctctaccac tgagctactg
     2221 tggattaata tt
//
LOCUS       DAKTIO010000260         2231 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.268, whole genome shotgun sequence.
ACCESSION   DAKTIO010000260 DAKTIO010000000
VERSION     DAKTIO010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.268"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..1370
                     /locus_tag="O0293_002717"
     CDS             <1..1370
                     /locus_tag="O0293_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HCY0281535.1"
                     /translation="IQLLAFAFIIMFILNPLVVYDIGFQFSFIISFFIMLLFPFLQQL
                     SKLQSLFIITFIAQLASFIVAIPSFHQLQWVGFLSNLIFVPYYSIILFPLSILFFITS
                     HFIVGLTPLNYLVDLSFNFHDWLLDLFTRIKQSHFSVPKFNDWIFIVFIISVYYIFWL
                     LAKRKYILVTFWTIIILTLLITFPTNSHHKITMLNVGQGDSILYEGGKNQNVLIDTGG
                     KVIDDTKQPSYSISKYHILPTLNERGINELEYLILTHPHNDHIGEVEYIISHIKIKHI
                     VIYNKGYSSNTLMLLSKLSHKYNIKLMDVRQVSSFKLGDSSFLFFDSFIPNSRDKNEY
                     SIITMITYQNKKVLLMGDASKNNESLLLKKYNLPEIDILKVGHHGSKTSSSKEFIEMI
                     KPKISLISSGKNNMYHLPNIEVVKRLQRIRSRIYNSQQNGQVTIDLDDNLKVDSSSYG
                     NASGL"
     gene            1427..>2231
                     /gene="holA"
                     /locus_tag="O0293_002718"
     CDS             1427..>2231
                     /gene="holA"
                     /locus_tag="O0293_002718"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCY0281536.1"
                     /translation="MSDNIVAIYGDVPELVEKQSAEIISQFLKSDRDDFNFVKYNLYE
                     TEIAPIVEETLTLPFFSDKKAILVKNAYIFTGEKAPKDMAHNVDQLIEFIEKYDGENL
                     IVFEIYQNKLDERKKLTKTLKKHARLKKIEQMSEEEIKKWIQSKLNENFKDIKRDALD
                     LFIELTGINFNIVSQEIEKLILFLGDRPTINKQDVNQIINRSLEQNVFLLTEYIQKRK
                     KEQAIHLVKDLITMKEEPIKLLALITSNYRLFYQCKILSQKGYSGQQIAK"
BASE COUNT          846 a          263 c          354 g          768 t
ORIGIN      
        1 gtattcagct attagcattt gcatttataa ttatgtttat tttaaatcca ctagttgttt
       61 atgatattgg atttcaattt tcattcatca tttcattttt tattatgcta ctttttcctt
      121 ttttacagca attgtcaaag ttacaatcat tattcataat tacgtttatt gcacaattag
      181 cttcatttat cgttgccatt ccaagctttc atcaacttca atgggtggga tttttatcta
      241 atttgatttt tgtaccgtac tattcgatta tattgtttcc gctatctatt ttattcttta
      301 ttacaagtca ttttattgtg ggattaacgc cgctaaatta cttggttgac ctaagtttta
      361 attttcatga ctggttacta gacctattca caagaatcaa gcaatcacat ttttctgttc
      421 ccaagtttaa tgattggata tttatagtat ttataatttc tgtttattac atattttggt
      481 tattggctaa acgtaaatat atattggtta cgttttggac tataattatt ctgacattat
      541 taataacgtt tccaacaaat tcacatcaca aaattacaat gttaaatgtg gggcagggag
      601 acagtatttt atatgaaggc ggtaagaacc aaaatgtctt gattgataca ggtgggaaag
      661 tgattgatga tactaaacaa cctagttatt caatttctaa atatcatatt ttaccaacgc
      721 taaatgaaag agggataaat gaattagagt atctaatttt aacacatcca cacaatgacc
      781 atattggtga agtggaatat attattagtc atattaaaat taaacatata gtgatataca
      841 ataagggata tagtagtaat acattgatgt tattatcgaa attaagccat aagtacaaca
      901 ttaaacttat ggatgtaaga caagttagta gttttaaact tggagatagt agttttctat
      961 tttttgatag ttttattcca aatagccgag ataaaaatga gtattcgatt attactatga
     1021 ttacatatca aaataaaaaa gttttattaa tgggcgatgc tagtaaaaat aatgaatctt
     1081 tactactaaa aaaatataac ttgccggaga ttgatatttt aaaagtagga catcatggga
     1141 gcaagacaag tagttctaaa gaatttatag agatgattaa gcctaaaata agtttgattt
     1201 cttctgggaa gaacaatatg tatcatcttc ctaatataga agttgttaaa cgattgcaaa
     1261 ggattcgcag tcgcatttac aatagtcaac aaaacggtca agttacaatt gacttagatg
     1321 ataatttaaa agttgattca agctcttatg gaaatgcaag tggtttatag atacagaaac
     1381 atgatataat ctattgatgt gtcaaacgaa tgaaagggtg taatgaatga gcgacaatat
     1441 tgtagctatt tatggagatg tgcctgaatt ggttgaaaaa caaagtgcag aaatcatatc
     1501 acaatttttg aaaagtgata gagatgactt taactttgtg aaatataatt tatacgaaac
     1561 agagattgca ccaattgttg aagaaacatt aacattgcct ttcttttcag ataaaaaagc
     1621 aattttggtt aaaaatgcat atatatttac aggtgaaaaa gcgccaaaag atatggctca
     1681 taatgtagat caattaatag aatttattga aaaatatgat ggcgaaaatt tgattgtctt
     1741 tgagatatat caaaataaac ttgatgaaag aaaaaagtta actaaaactc taaaaaagca
     1801 tgcaaggctt aaaaaaatag agcaaatgtc tgaagaagaa ataaaaaaat ggattcaaag
     1861 taaattaaat gagaatttca aagatatcaa aagagatgca ttagatttat ttattgagtt
     1921 gacaggtatt aactttaata ttgtctcaca agagatagaa aagttgattt tatttttagg
     1981 cgatagacca acaattaata agcaggatgt taaccaaatt attaatagaa gtttagagca
     2041 aaatgtattt ttactgactg aatacattca gaaaagaaag aaagaacaag caattcattt
     2101 agtaaaagat ttaataacta tgaaagaaga accaattaaa ttacttgcac taattacaag
     2161 taattaccga ttattttatc aatgtaagat tctgagtcaa aaaggatata gtggacagca
     2221 aattgctaaa a
//
LOCUS       DAKTIO010000261         2206 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.269, whole genome shotgun sequence.
ACCESSION   DAKTIO010000261 DAKTIO010000000
VERSION     DAKTIO010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.269"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..772
                     /locus_tag="O0293_002719"
     CDS             <1..772
                     /locus_tag="O0293_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0281537.1"
                     /translation="SIHYSTLGEGYPIVLIHTVLDNYSVFNKLAAELAKSFQVVLIDL
                     RGHGYSDKPRHIEIKDFSDDIVELLKYLYIEEVAFVCHEMGGIIGADISVRYPEFTSS
                     LTLVNPTSIEGELPEERLFRKYAHIIRNWDPEKQDKFLNKRKYYRPRKMNRFLKHVVD
                     TNEISTKEEIQAVKEVFKNADISQTYRNVVVPTKIIAGEFGERTTRLEAKEVADLIQN
                     ADFEVYQESSAFPFVEEQERFVEDTAAFINKHHDEKHV"
     gene            908..1414
                     /locus_tag="O0293_002720"
     CDS             908..1414
                     /locus_tag="O0293_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281538.1"
                     /translation="MKKLLTASIIACSVVMGVGLVNTSAEAASGNSIDTVKQLIKGDQ
                     SLENVKIGESIKDVLTKYKNPMYSYNEDGTEHYYEFHTKKGMLLVTTDGKKNNGKVTH
                     ISMMYNDANGPTYQAVKNYVGKAVTHTEYSKVAGNFGYIEKGKTTYQFASAPKDKNIK
                     LYRIDLEK"
     gene            1576..>2206
                     /locus_tag="O0293_002721"
     CDS             1576..>2206
                     /locus_tag="O0293_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281539.1"
                     /translation="MRINDKILLENIEDYFNHKGLSPHLIDDIKEKVITDIKNSEKKD
                     QDYIEYKRKSPAQIILMIQRNLFALQMNPVIFFIINFILISYLYDKQYVQFQAITGMS
                     LFYCLVIFPMTIVVYLRVSQKNYLRSNKIEMIMGTIIAIISLLLIILQAFNITWGVIP
                     ITNFGHQFFFFIGIILVIAGIFYKRLEFSGIGLLFCQKTVDAMIHNPQSA"
BASE COUNT          826 a          283 c          372 g          725 t
ORIGIN      
        1 atcgatacat tacagtacat taggtgaagg ctatcctatc gtattgattc atactgtact
       61 tgataattat tctgtgttta ataaattagc agcagaatta gcaaaatcat ttcaagttgt
      121 gttaattgat ttacgtggac atggctattc tgataaacct cgtcacattg aaataaaaga
      181 tttttctgat gacattgttg aattacttaa atatttatat attgaagaag ttgcatttgt
      241 atgccatgaa atgggtggaa tcattggtgc ggatatttca gtacgttatc ctgaatttac
      301 atcatcactt acgttggtaa atccaacatc tattgaaggt gaattaccgg aagaacgttt
      361 atttagaaaa tatgcccata ttattcgaaa ctgggatcct gaaaaacaag ataaattttt
      421 aaataagcgt aagtattatc gtccgagaaa aatgaatcga ttcctcaaac atgtcgtaga
      481 tacaaatgaa atatcaacta aagaagaaat tcaagcagtt aaagaggtat tcaaaaacgc
      541 tgatatttct caaacttata gaaatgtcgt agtaccgaca aaaattattg caggagaatt
      601 cggtgaaaga acaacaagat tagaagctaa agaagtagct gatttaatcc aaaatgcgga
      661 ctttgaagta tatcaagaat caagtgcatt cccatttgtt gaagagcaag aaagattcgt
      721 cgaagataca gctgcattta tcaacaaaca tcacgatgaa aagcatgttt aatttttagt
      781 gataagtgaa atataaaagt agtaattatt atttgtaata taattgtaat atgactgttg
      841 ttttagaaat gattgttgta taatggttgt gagcacaaga cgatataaat aaaaggagag
      901 agtgcttatg aaaaagctac taacggcaag tataattgca tgttctgttg taatgggagt
      961 aggcttagtg aacactagtg ccgaagcagc aagtggcaac tctattgata ctgttaaaca
     1021 attaattaag ggtgatcagt cattagaaaa tgtgaaaatt ggcgaatcta ttaaagatgt
     1081 tttaactaag tacaaaaatc cgatgtattc ttacaatgaa gatggaactg aacattatta
     1141 cgaattccat actaaaaaag gtatgttatt agtaactact gatggtaaga aaaacaatgg
     1201 taaagtaact catatttcaa tgatgtataa tgatgctaat ggtccaacat atcaagctgt
     1261 taaaaattat gttggcaaag cagtaacaca tacggaatat agcaaagttg ctggtaattt
     1321 tggatatatt gaaaaaggca aaacgactta tcaatttgcc tcagcaccaa aagataaaaa
     1381 cataaaatta tatcgtattg atttggaaaa ataataatta aattaatcgt gcttaaccga
     1441 caagttaaat cgcaatactt gtcggttatt ttattacatg gcataataca gtgaatatta
     1501 atttttatga tttgcgaagg ggaaattaaa ccaatacaat aaataaagat gtttcatttt
     1561 aaaggagtac taccaatgcg tattaacgat aaaattttac tagaaaatat agaagattac
     1621 tttaatcata aaggtttatc accgcatttg attgatgata ttaaagagaa agtaattact
     1681 gatataaaaa attctgaaaa gaaagatcaa gattatattg aatataaaag aaaatctcca
     1741 gcacaaatca tattaatgat tcaaaggaat ttgtttgctt tacaaatgaa tccagttatt
     1801 ttctttatta taaacttcat tctcatatcc tatttatatg ataaacagta tgttcagttt
     1861 caagctatta ctggaatgag tctattttat tgtttagtga tttttccaat gactattgtt
     1921 gtatacctaa gggtgtctca aaagaattac ttgcgtagta ataaaataga aatgattatg
     1981 ggtacaatta tcgctatcat atccttgtta ttaattatat tacaagcatt taatattact
     2041 tggggcgtta taccaattac aaattttgga catcaatttt tctttttcat tggtattatt
     2101 ttagtaattg ccggcatatt ttataagcga cttgagtttt cgggaatcgg gttattattt
     2161 tgtcaaaaaa ccgtcgatgc aatgattcat aatccacaat cagccc
//
LOCUS       DAKTIO010000262         2198 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.270, whole genome shotgun sequence.
ACCESSION   DAKTIO010000262 DAKTIO010000000
VERSION     DAKTIO010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.270"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(733..1932)
                     /locus_tag="O0293_002722"
     CDS             complement(733..1932)
                     /locus_tag="O0293_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCY0281540.1"
                     /translation="MSNTQKKNVPELRFPGFEGEWEEKKLGNLTTKIGSGKTPKGGSE
                     NYTNKGIPFLRSQNIRNGKLNLNDLVYISKDIDDEMKNSRTYYGDVLLNITGASIGRT
                     AINSIVETHANLNQHVCIIRLKKEYYYNFFGQYLLSRKGKRKIFLAQSGGSREGLNFK
                     EIANLKIFTPTIFEEQQKIGKFFSKLDRQIELEEQKLELLQQQKKGYMQKIFTQELRF
                     KDENGEEYPEWENKFIKDIFIFENNRRKPITSSLREKGLYPYYGATGIIDYVKDYLFN
                     NEERLLIGEDGAKWGQFETSSFIANGQYWVNNHAHVVKSNDHNLFFMNYYLNFKELRA
                     FVTGNAPAKLTHANLCNINLKIPCLTEQDKVSALLKSIDNKMNNQMNRIELLKERKKG
                     LLQKMFI"
     gene            complement(1925..>2198)
                     /locus_tag="O0293_002723"
     CDS             complement(1925..>2198)
                     /locus_tag="O0293_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HCY0281541.1"
                     /translation="HLSDAQVERIIDTYKRKETIDKYSYSATLQEIADNDYNLNIPRY
                     VDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAYLKELGVLKDE"
BASE COUNT          622 a          368 c          282 g          926 t
ORIGIN      
        1 cactattcat ttgaacatgg tctaaaacga ataggacatt gtaaatgatt aaatagtctg
       61 tctctagttg atgtatatgg tcatttgtta tgcagataat aattaatcta attaattttt
      121 acgttttagt tacttggttg tgattgaggc tttttgtatc tagttgtaat attctccttt
      181 tttagaatca taataggtac ttcttgaaat ttttagaact ttgcacattg ctgatactga
      241 atattgatac gcattttgtt gaaagacttc attttttgtc tcaaaattag cactgcttgc
      301 tttaaatcat cattttacat tatcaatcga tgtttttctt tgcgtaattt tattaactct
      361 ttttcatcac ttgttaaaat gtcttgatga ttgaatgact cagtattttg gttttgtttt
      421 atctaatttg agaaagttaa tgatgttaga tcatattctc gtataatttc gtttctaagc
      481 ttaccatttt cataaagttt aattatttta atttaaatta tttactaaaa gctcttcagt
      541 ctcttgtcat aataaaatcg cctaccctct tagtttaaca atgtctattc tcatagaatt
      601 tgtccaacta aatgtagacg attcactaaa cttctaaaaa aataaacccc aaattataat
      661 agtctcaata ttaatatatt acaatttatt cataggattt ttatcatttc aattaatagg
      721 ttgttatcag ggttaaataa acattttttg taatagtcct tttttacgtt cttttaataa
      781 ctcaattcta ttcatttgat tattcatttt attgtctata gattttaaca atgcacttac
      841 tttatcttgt tcagtgagac aaggtatttt aagatttata ttgcataagt tcgcatgagt
      901 taatttagct ggtgcattac ctgtgacaaa tgctcgtagt tctttaaaat ttaaataata
      961 attcataaaa aacaaattat gatcattact tttaactaca tgcgcatgat tatttaccca
     1021 gtattgccca ttagcaataa agctactcgt ctcaaactgc ccccattttg caccatcttc
     1081 tcctattagt aatcgttctt cattattgaa taaataatct tttacgtaat caataattcc
     1141 agttgcacca tagtaagggt ataacccctt ttctcttaat gaagaagtaa ttggttttct
     1201 tctattattt tcaaagataa agatgtcttt tatgaatttg ttttcccact ctggatattc
     1261 ttcaccattc tcatctttga atcgcaattc ttgtgtgaag attttctgca tatagccttt
     1321 tttctgttgt tgaagcaatt caagtttttg ttcttctaat tcaatttgtc ggtcgagttt
     1381 gctgaagaac ttgcctattt tttgctgttc ttcaaatata gttggggtga agatttttaa
     1441 attagcaatt tctttgaagt ttagaccttc tcgactacct ccactttgtg caaggaaaat
     1501 tttcctttta ccttttcttg ataatagata ctgtccaaaa aaattataat aatactcttt
     1561 tttcaatcta ataatacata catgttgatt taaattagca tgcgtttcaa ctatcgaatt
     1621 aatggctgtt ctacctattg atgctcctgt aatatttaaa agaacatcac catagtacgt
     1681 tctactattt ttcatctcat catctatatc tttactaata taaactaagt cattaagatt
     1741 taatttacca tttctaatat tttgactcct taaaaatggt atgcctttgt ttgtatagtt
     1801 ttcacttcca cctttgggag tctttccact acctattttg gtagtaagat tccctaactt
     1861 cttctcttcc cattcgcctt caaatcctgg gaatctcaat tctggcacat ttttcttttg
     1921 tgtattactc atctttcaac accccaagtt ctttcaggta tgcattgatt tcttgctcaa
     1981 tttctgcgat ttctttatcg atatttttca aatcttgttg gacttgatct aaatcaatcg
     2041 gtgcttcttc ttcgaatgta tcgacatatc tcggtatatt taggttgtaa tcgttatcgg
     2101 caatctcttg tagtgtcgcg ctgtagctat atttatcaat tgtttcctta cgcttatatg
     2161 tgtctataat acgttcgact tgggcatcgc ttaaatga
//
LOCUS       DAKTIO010000263         2187 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.271, whole genome shotgun sequence.
ACCESSION   DAKTIO010000263 DAKTIO010000000
VERSION     DAKTIO010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.271"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            351..1253
                     /locus_tag="O0293_002724"
     CDS             351..1253
                     /locus_tag="O0293_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCY0281542.1"
                     /translation="MKQYLITGGTGMVGSQLVNEIKKSDSHITILTRHDQISNDKKIS
                     YVNWAKSGWEHKVPQNIDVVINLAGATLNKRWTPEYKQTLMLSRIQSTQALYELFKSR
                     NKAPKALFNASATGYYPPDLFMSYTEVYKTLPFDFLSDIVYQWERFAQQFEQLGTRVV
                     IGRFGIILSNEGGALQTMKLPYEYYIGGKLGSGQQWYSWIHINDLIQAILFLINNESA
                     SGPFNLTAPIPERQNLFGYTLARAMHKPHETWAPSLAMRLILGQMSTVVLDTQKVLPN
                     KIQALGFQFKYSNLKMALEDLIKE"
     gene            complement(1496..2083)
                     /gene="clpP"
                     /locus_tag="O0293_002725"
     CDS             complement(1496..2083)
                     /gene="clpP"
                     /locus_tag="O0293_002725"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCY0281543.1"
                     /translation="MNLIPTVIETTNRGERAYDIYSRLLKDRIIMLGSQIDDNVANSI
                     VSQLLFLQAQDSEKDIYLYINSPGGSVTAGFAIYDTIQHIKPDVQTICIGMAASMGSF
                     LLAAGAKGKRFALPNAEVMIHQPLGGAQGQATEIEIAANHILKTREKLNRILSERTGQ
                     SIEKIQKDTDRDNFLTAEEAKEYGLIDEVMVPETK"
BASE COUNT          735 a          356 c          340 g          756 t
ORIGIN      
        1 ttatattact atgtattctt aaatttgttt aaaaaattaa tgctttaaat tgatgtataa
       61 tgggaataag aaataaataa aaacgacccg cacgattaac gtacgggtcc actactaaag
      121 ggagtcaaat ttttaccttc gtttgtatca tgcagcgttt tacacatact tttaagagat
      181 gtttattcgt tatcgaaggt acacctttat tataacttat atcattttta ttaaaataca
      241 attcaaaaaa agttattgac atattattgt ctaaataatg tataaccttt gaacaacaat
      301 aatgattatt aaatagtaac taaatacaaa attacatggg gtgaatgata atgaaacaat
      361 acttaattac tggtgggact ggtatggttg gatctcaatt agttaatgaa attaaaaaat
      421 cagattcaca tatcacgata ttaacgcgac acgaccaaat ttcaaatgat aagaaaattt
      481 catatgtcaa ctgggctaaa tctgggtggg aacacaaagt tcctcaaaat atcgatgtgg
      541 tcatcaactt agcaggtgct acattgaata aacgatggac accggaatat aaacaaacgc
      601 taatgctaag tagaattcaa tctacgcaag ctttatatga attgtttaaa tcacgtaata
      661 aagcacctaa agctttattt aatgctagtg ccactggtta ctatcctcct gatttattta
      721 tgagttacac agaagtttat aaaactttac cttttgattt cttatcagat attgtgtatc
      781 aatgggaacg tttcgcacaa caatttgaac aattaggtac acgagttgtc atcggtcgtt
      841 tcggtataat tttatcgaat gaaggcggtg cgttacaaac aatgaaacta ccatacgaat
      901 attacattgg tggtaaatta ggttctggtc aacaatggta ttcatggatt catatcaatg
      961 atttaattca agctatttta tttttaataa ataacgagtc agctagtggt ccgtttaatt
     1021 taactgcacc tatacctgaa cgtcaaaatt tatttggcta cactttagca agagctatgc
     1081 ataagcctca tgaaacttgg gcaccaagtc ttgcaatgcg tctcatactt ggtcaaatgt
     1141 caacagtagt attggatact caaaaagtat tacctaataa aattcaagca ttgggattcc
     1201 aatttaaata tagtaattta aaaatggcac ttgaagattt aattaaagaa taatcaatac
     1261 cattaatgag cattagaaac aacatatgta ctaaatgtaa tgtctagagc gacttacaaa
     1321 caaaggattg aaatgttcct ttcaatgtct actttgaagg gaacatttca tatagttacg
     1381 gttcaactag cttcattcaa tcatatttcc gctcaaagta tagtaaacaa attattgaca
     1441 ccttggttta ctcatacaaa aagagcagac agcttagtct actctttact ttgaattatt
     1501 ttgtttcagg taccatcact tcatcaatta agccatattc tttagcttct tctgcagtta
     1561 agaagttatc acgatctgtg tctttttgta ttttttcaat actttgacca gtacgctctg
     1621 ataaaatgcg gtttaatttt tcacgtgttt ttaaaatgtg atttgcagca atttcgattt
     1681 cagttgcttg tccttgagca ccacctaatg gttggtgaat cattacttct gcatttggta
     1741 acgcgaaacg tttacctttt gcaccagctg ctaataagaa tgatcccatt gatgcagcca
     1801 taccgataca aattgtttga acatcaggtt taatgtgttg aattgtatca taaatcgcaa
     1861 aaccagctgt tacacttcca cctggtgaat taatgtataa ataaatatct ttctctgagt
     1921 cttgcgcttg taagaataat aactgtgata cgattgaatt tgctacgttg tcatcaattt
     1981 gtgaacctaa cataataata cggtctttta ataaacgtga gtatatatca tatgcacgtt
     2041 caccgcggtt tgttgtttca ataactgtag gaattaaatt catctatatt tcctccttgt
     2101 aataactgtt actcattatt ttacatatta agtcaaattt ggtcaaacac tttgacttta
     2161 aatagttaca ttgtttgacg cttttct
//
LOCUS       DAKTIO010000264         2162 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.272, whole genome shotgun sequence.
ACCESSION   DAKTIO010000264 DAKTIO010000000
VERSION     DAKTIO010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.272"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            45..680
                     /gene="sdpA"
                     /locus_tag="O0293_002726"
     CDS             45..680
                     /gene="sdpA"
                     /locus_tag="O0293_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpA"
                     /protein_id="HCY0281544.1"
                     /translation="MQKFKDFFYDDLSVTRGNYFLTLMAAFFITIILFIGIVVSEVHL
                     LYSMLIVLVGLILLRLFKINLFSFKKLTLSQVIYIIGGALLIYGLDNLYLYFHDVPAN
                     EQQLEQAIRNTPFYISIFTVTIIPAIVEEIVFRGMIIRVIFRKHLFLGLIVSSLVFAS
                     LHESDTWIGYLPYLYSGLIFGLIYIKTKRLEVVIFMHFLNNLLALLFIIWR"
     gene            complement(1120..1833)
                     /locus_tag="O0293_002727"
     CDS             complement(1120..1833)
                     /locus_tag="O0293_002727"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCY0281545.1"
                     /translation="MAKLNIEVFADGADIEEMKAAYKNKQVDGFTTNPSLMAKAGVTD
                     YKAFAEEAVKEIPDASISFEVFADDLETMEKEAAILKQYGENVFVKIPIVNTKGESTI
                     PLIKKLSADNVRLNVTAVYTIEQVKEITEAVTEGVPTYVSVFAGRIADTGVDPLPLMK
                     EAVKVTHSKDGVKLLWASCRELFNVIQADEIGADIITCPADVVKKVNTNLGRDINELS
                     VDTVKGFAKDIQSSGLSIL"
BASE COUNT          684 a          347 c          277 g          854 t
ORIGIN      
        1 ttgcattcaa aataatttta aaaaatgaaa agaaaggatt tagcatgcaa aaattcaaag
       61 acttttttta cgatgattta tcggttacac gaggaaatta ttttttaact ttaatggcag
      121 cattttttat tactatcatt ttatttatcg gcatagttgt cagtgaagta catttacttt
      181 atagcatgct aattgtatta gtaggtttaa ttctattgag gctattcaaa atcaatttat
      241 tctcttttaa aaaattaaca ttgtctcaag ttatttatat tataggcggt gcactattaa
      301 tttatgggtt agataatctt tatttatatt ttcatgacgt accggcaaat gaacaacaat
      361 tagagcaagc aatacgaaat acaccattct atatttctat tttcactgtt accatcatcc
      421 ccgctattgt ggaagaaatt gtttttcgcg gtatgataat aagggttatc ttcagaaaac
      481 acttgttttt agggttaatt gtgtctagtt tagtttttgc atcattacac gaatctgaca
      541 cttggattgg ttatttacct tacttatatt ctggtttgat ttttggccta atttatataa
      601 aaacaaaacg attagaagtt gtaatattca tgcacttctt aaataacttg ttagctctgc
      661 tctttataat atggagataa ttttgagata acctttcata ttattttcaa attatctatc
      721 tgatatttat tttattgaac ttgagtcacc acttaaactc aaaaatacgt gtcatcaagt
      781 gcaaaataat ttagaactaa tacaaacata aaaactttta ctttacgctt aaaatcaaaa
      841 gtcacatacc tagatataag tacaacttgt atattttgta ctctaatagg tatgtggctt
      901 aaataatatt taattcttga gttgcatata gtaactgttt ttattcattt tctaaaatat
      961 gcaagactaa tttccttttc tactcctgtt gaatcaattt agactttttt ataactcgag
     1021 cttttactta gctgaatttt gacttcttct tcattaataa ctatgatgga aaaaccccca
     1081 taagcacaat gcctatgagg gtttaaatat atttatatat tatagaatag aaagacctga
     1141 agattgaata tctttcgcaa agcctttaac tgtatctact gataattcgt taatatcgcg
     1201 acctaagttt gtattcactt ttttcacaac atctgctggg catgtaataa tatctgcacc
     1261 aatttcatca gcttgaatca cattgaataa ttcgcggcaa cttgcccata ataatttaac
     1321 gccgtcttta ctatgcgtaa cttttacagc ctctttcatt aatggtaatg gatctacgcc
     1381 tgtatctgca atacgtcctg caaatactga aacatatgtt ggcacacctt cagttactgc
     1441 ttcagttatt tctttaactt gttcaattgt gtaaacagcc gtaacgttta atctcacatt
     1501 gtcagctgaa agttttttaa ttaaaggaat cgttgattca ccttttgtat ttacaatagg
     1561 aattttaaca aatacatttt cgccatattg ttttaaaatt gctgcttctt tttccatagt
     1621 ttctaaatcg tctgcaaata cttcaaatga aattgaagca tctggaattt ctttcacagc
     1681 ttcttcagca aaagctttgt aatctgttac gcccgcttta gccattaaac taggatttgt
     1741 tgtaaaacca tccacttgtt tgtttttata agctgctttc atttcttcaa tatctgcacc
     1801 gtccgcaaat acttctatat ttagtttagc catataatat agcctccttg attcttatta
     1861 aaattttaac aacatctgca tgtctttttc ttacaaccat ttgtaaaaaa tgatttttat
     1921 ttctttgtta tataaaacat ttatatcacg cttttataat atatagctaa ttttttgcct
     1981 atatttttta aaaatgactt tagcaaacat tgtgtctaca agttaataaa actattgttt
     2041 tatgattgtt tatgatacga tatgtaaatg ataatcatcc aggaggtcta gcatgtctcg
     2101 ttcaaaaaaa tacttttact tatctagctt aatgattatt ttaagctttt tctttaatac
     2161 aa
//
LOCUS       DAKTIO010000265         2151 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.273, whole genome shotgun sequence.
ACCESSION   DAKTIO010000265 DAKTIO010000000
VERSION     DAKTIO010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.273"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            37..828
                     /gene="pgeF"
                     /locus_tag="O0293_002728"
     CDS             37..828
                     /gene="pgeF"
                     /locus_tag="O0293_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HCY0281546.1"
                     /translation="MNDNFKKQPHHLIYEELLQQGITLGITTRGDGLSDYPKNAFNMA
                     RYIDDRPYNITQHQLQLAEEIAFDRKNWVFPIQTHENKVACITKDDIGTNIDTLTDAL
                     HGIDAMYTYDSNVLLTMCYADCVPVYFYSTKHHFIALAHAGWRGTYTEIVKEVLKHVN
                     FDLKDLHVVIGPSTSSSYEINDDIKNKFETLPIDSANYIETRGRDRHGIDLKKANAAL
                     LIYYGVPKENIYTTAYATSEHLELFFSYRLEKGQTGRMLAFIGQQ"
     gene            852..1520
                     /locus_tag="O0293_002729"
     CDS             852..1520
                     /locus_tag="O0293_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HCY0281547.1"
                     /translation="MKDNLQQISTQINDKSEKNNFSTKPNVIAVTKYVTIERAKEAYE
                     AGIRHFGENRLEGFFQKKEALPSDAVIHFIGSLQSRKVKDVINDVDYFHALDRLSLAK
                     EINKRAEHKIKCFLQVNVSGEASKHGIALEDVDQFIDDLKKYDKIEIVGLMTMAPLTD
                     DEAYIRSLFKQLRLKKEEIQRLNLEYAPCDELSMGMSNDYLIAVEEGATFVRIGTKLV
                     GEEE"
     gene            1517..2080
                     /locus_tag="O0293_002730"
     CDS             1517..2080
                     /locus_tag="O0293_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HCY0281548.1"
                     /translation="MSHLALKDLFSGFFVIDDEEEVEVPDKQQQVNEAPAKEQSQQTT
                     KQNAIKSVPQKSASRYTTTSEERNNRMSNYSKNNSRNVVTMNNATPNNASQESSKMCL
                     FEPRVFSDTQDIADELKNRRATLVNLQRIDKVSAKRIIDFLSGTVYAIGGDIQRVGTD
                     IFLCTPDNVEVAGSITDHIENMEHSFD"
BASE COUNT          804 a          300 c          408 g          639 t
ORIGIN      
        1 cgaagataat ttaaatgaaa tgatggtgac gatcgagtga atgataattt taaaaagcaa
       61 ccgcatcatt taatatatga agagttatta caacaaggta ttactctagg tattacaact
      121 agaggagatg gtttaagtga ctatcctaaa aatgctttta atatggcgag atatattgat
      181 gatcgcccat ataatattac tcaacatcaa ttgcaattag ctgaagaaat tgcgtttgat
      241 agaaaaaatt gggtgtttcc cattcaaaca catgaaaata aagtcgcttg tattacaaag
      301 gatgatatag gcacaaatat agacacttta actgatgcgc ttcatggtat tgatgcgatg
      361 tacacatatg atagtaatgt cttattaacg atgtgttatg cagactgtgt accagtatat
      421 ttttatagta caaaacatca ttttattgca ttggcgcatg caggttggcg tggtacctat
      481 actgaaattg taaaagaagt gctaaaacat gtgaactttg atttgaaaga cttacatgtc
      541 gttattggac catctacatc atcaagttat gaaattaatg atgatattaa aaataaattt
      601 gaaacattgc caattgatag tgccaactat attgaaacta gaggacgaga tcgtcatggt
      661 attgatttga aaaaagccaa tgctgcatta ttaatttatt atggtgttcc taaagaaaat
      721 atttatacga cagcgtatgc tacatctgaa catttagaat tatttttctc ttatcgatta
      781 gaaaaaggtc aaacaggacg catgttagca ttcattggtc aacagtaaac aaggaggaga
      841 tatgtttgcg tgtgaaagat aatttacaac aaatctcaac acaaattaat gacaaaagtg
      901 aaaaaaataa tttttcaaca aaaccaaacg tgattgcagt tacaaaatat gttacaatag
      961 agcgagctaa agaagcgtat gaggctggaa taagacattt tggtgagaat agattggaag
     1021 gcttttttca aaagaaagaa gcattaccat cagatgcggt gatccatttt ataggatcat
     1081 tacaatctcg aaaagttaag gacgttataa acgacgtaga ttatttccat gctttagatc
     1141 gattgagctt agccaaagaa attaacaaac gtgcagaaca taaaattaaa tgtttcttgc
     1201 aagtgaacgt ttcgggagaa gcttctaaac atggtattgc tttagaagat gttgatcagt
     1261 ttatagatga tcttaaaaaa tatgacaaaa tcgaaattgt aggtttaatg acgatggcac
     1321 cattgacaga tgatgaagca tatattagat cgttatttaa acagttacgt ttgaaaaaag
     1381 aagaaataca acgactcaat ttagaatatg cgccttgtga tgaattatca atgggaatga
     1441 gtaatgacta tcttattgca gttgaagaag gtgcgacgtt tgttagaatt gggactaaac
     1501 ttgtaggaga agaggagtga gccacttggc tttaaaagat ttatttagtg gattttttgt
     1561 aatagatgat gaagaggaag tagaagtacc tgacaaacaa caacaggtaa atgaagcgcc
     1621 agcaaaagag cagtcacaac aaacaacaaa acaaaacgca atcaaatcag tccctcaaaa
     1681 atctgcatca agatatacaa caacgtcaga agaaaggaat aaccgtatgt ctaattattc
     1741 aaaaaataat tcacgtaatg ttgtaactat gaacaatgct acaccaaaca atgcatcaca
     1801 agaaagttca aaaatgtgtt tattcgaacc acgtgttttt tcagatacac aagatattgc
     1861 tgatgagctt aaaaaccgcc gtgcgacact tgtcaattta caacgtattg ataaagtatc
     1921 agcgaaaaga attattgatt ttttaagcgg tactgtttat gcaatcggtg gagatatcca
     1981 acgtgtaggt actgatattt tcttatgtac gcctgataat gtggaagtag ctggaagcat
     2041 tacagaccat attgaaaata tggaacattc attcgactaa ggagttaaca tatggatata
     2101 aatgtgctag ctacaatatt taaattcatc ctttttgttg ttgaaattta t
//
LOCUS       DAKTIO010000266         2142 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.274, whole genome shotgun sequence.
ACCESSION   DAKTIO010000266 DAKTIO010000000
VERSION     DAKTIO010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.274"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            5..952
                     /gene="hlgC"
                     /locus_tag="O0293_002731"
     CDS             5..952
                     /gene="hlgC"
                     /locus_tag="O0293_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgCB subunit C"
                     /protein_id="HCY0281549.1"
                     /translation="MLKNKILTTTLSVSLLAPLANPLLENAKAANDTEDIGKGSDIEI
                     IKRTEDKTSNKWGVTQNIQFDFVKDKKYNKDALILKMQGFISSRTTYYNYKKTNHVKA
                     MRWPFQYNIGLKTNDKYVSLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGGNGSF
                     NYSKSISYTQQNYVSEVEQQNSKSVLWGVKANSFATESGQKSAFDSDLFVGYKPHSKD
                     PRDYFVPDSELPPLVQSGFNPSFIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTH
                     YGNSYLDGHRVHNAFVNRNYTVKYEVNWKTHEIKVKGQN"
     gene            954..1931
                     /gene="hlgB"
                     /locus_tag="O0293_002732"
     CDS             954..1931
                     /gene="hlgB"
                     /locus_tag="O0293_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB/HlgCB subunit
                     B"
                     /protein_id="HCY0281550.1"
                     /translation="MKMNKLVKSSVATSMALLLLSGTANAEGKITPVSVKKVDDKVTL
                     YKTTATADSDKFKISQILTFNFIKDKSYDKDTLVLKATGNINSGFVKPNPNDYDFSKL
                     YWGAKYNVSISSQSNDSVNVVDYAPKNQNEEFQVQNTLGYTFGGDISISNGLSGGLNG
                     NTAFSETINYKQESYRTTLSRNTNYKNVGWGVEAHKIMNNGWGPYGRDSFHPTYGNEL
                     FLAGRQSSAYAGQNFIAQHQMPLLSRSNFNPEFLSVLSHRQDGAKKSKITVTYQREMD
                     LYQIRWNGFYWAGANYKNFKTRTFKSTYEIDWENHKVKLLDTKETENNK"
     gene            complement(1983..>2142)
                     /locus_tag="O0293_002733"
     CDS             complement(1983..>2142)
                     /locus_tag="O0293_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCY0281551.1"
                     /translation="LGSLVSRLLLSIILNLPVWVVLLNAIPGVIFTLIVAIPLYLTLR
                     KRMAVLLR"
BASE COUNT          833 a          347 c          350 g          612 t
ORIGIN      
        1 acttatgctt aaaaataaaa tattaactac aactttatct gtgagcttac ttgcccctct
       61 tgccaatccg ttattagaaa atgctaaagc tgctaacgat actgaagaca tcggtaaagg
      121 aagcgatata gaaattatca aaaggacaga agataaaaca agtaataaat ggggcgtgac
      181 tcaaaatatt caatttgatt ttgtaaagga taaaaaatat aacaaagatg ctttgatatt
      241 aaagatgcaa ggattcatta gctctagaac aacatattac aactataaaa aaactaatca
      301 tgttaaagct atgcgatggc cattccaata taatattggt ttaaaaacaa atgataaata
      361 tgtttcttta attaattatt tacctaaaaa taaaattgaa tctacaaacg tgagtcagac
      421 attaggatac aatatcggtg gtaatttcca atcagcccca tcactcggtg gtaatggatc
      481 atttaactat tctaaatcga ttagctatac acaacaaaat tatgtaagtg aagtagaaca
      541 acaaaactca aaaagtgttt tatggggcgt caaagcgaat tcattcgcca ctgaatcagg
      601 tcaaaaatca gcctttgata gcgatttatt tgtaggctac aaacctcata gtaaagatcc
      661 tagagattat ttcgttccag acagtgagtt accacctctt gtacaaagtg gatttaaccc
      721 ttcatttatc gccacagtat ctcatgaaaa aggttcaagc gatacaagcg aatttgaaat
      781 tacttacgga agaaacatgg atgtcactca tgccattaaa agatcaacgc attatggcaa
      841 cagttattta gacggacata gagtccataa tgcatttgta aatagaaact atactgtgaa
      901 atacgaggtc aattggaaga ctcatgaaat caaggtgaaa ggacagaatt gatatgaaaa
      961 tgaataaatt agtcaaatca tccgttgcta catctatggc attattatta ctttctggta
     1021 ctgctaatgc tgaaggtaaa ataacaccag tcagcgtaaa aaaagtcgat gacaaagtta
     1081 ctttatacaa aacaacagcc acagcagatt ctgataaatt taaaatttca cagattttaa
     1141 catttaattt catcaaagat aaaagttatg ataaagatac tttagtactt aaagctactg
     1201 ggaatattaa ctcaggcttt gtgaaaccta atcctaatga ctatgacttt tcaaaattat
     1261 attggggagc taaatacaat gtatctataa gctcacaatc taatgattca gtaaacgtcg
     1321 ttgattatgc accaaaaaat caaaatgaag agtttcaagt tcaaaatact ttaggctata
     1381 catttggtgg tgacattagt atctctaatg gtttatctgg tggacttaat ggaaatacag
     1441 ctttttctga aacaattaat tataaacaag aaagttacag aacaacatta agtcgcaaca
     1501 caaattataa aaatgttggc tggggagttg aagcacataa aattatgaat aatggttggg
     1561 gaccttatgg aagagatagc ttccacccaa catatggtaa tgaactcttc ttagctggca
     1621 gacaaagcag tgcatacgct ggccaaaact tcatagcgca acaccaaatg ccattattat
     1681 ctagaagtaa cttcaatcca gaatttttaa gcgtactatc acacagacaa gatggcgcta
     1741 aaaaatctaa aattacagta acttatcaac gtgaaatgga tttataccaa attcgttgga
     1801 atggcttcta ctgggcaggc gcaaattata aaaactttaa aactagaaca tttaaatcaa
     1861 catatgaaat tgattgggaa aatcacaaag tgaaattgtt agatacaaaa gaaactgaaa
     1921 acaataaata gctagtaaaa cacggtcgcc aacagtaatt gtgacgaccg tgttttgatt
     1981 tattatctta gtaatactgc cattcttttt ctcaatgtga gatataaagg aatagctaca
     2041 attaaagtga atattacgcc tggaatcgcg tttaacaaca ctacccacac aggtaaattt
     2101 aaaataatag ataatagtag cctagatacc aaactgccta at
//
LOCUS       DAKTIO010000267         2139 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.275, whole genome shotgun sequence.
ACCESSION   DAKTIO010000267 DAKTIO010000000
VERSION     DAKTIO010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.275"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            151..1188
                     /locus_tag="O0293_002734"
     CDS             151..1188
                     /locus_tag="O0293_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCY0281552.1"
                     /translation="MKTRCYDGKKWQYEFKYEGKRYRKKGFRTKREANSAGLDKLNEL
                     RSGFNIDNYITLEEYFENWIKTYKQPVVKENTYRHYRNALQHIQKHKIGKMELSKINR
                     QVYQKFINDYSKEHAKETIRKTNGAIRSALDDALYDGLIFKNPAYKVNYKAGKPTKSE
                     QEKFISVTEYEILKDHVRKKRTRSSLALFIMICTGCRVSGARNIKIEHINQVKNTIFI
                     DERKTDTSPRYISIAKSDMKHIMDVISTFAISYDGYIFKEAGSIINLHAINNALKSAC
                     RVNNISIITSHALRHTHCSYLLAKGVSIHYISKRLGHKNIAITTSVYSHLLEEKFNEE
                     DKKTTKILESM"
     gene            1239..2069
                     /gene="sph"
                     /locus_tag="O0293_002735"
                     /pseudo
     CDS             1239..2069
                     /gene="sph"
                     /locus_tag="O0293_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
BASE COUNT          825 a          329 c          365 g          620 t
ORIGIN      
        1 tatatattcg ttaccatttt tggtaattaa ccatgtaatc ttataactat aagtggcgtc
       61 tgtattttat acaggcgtct ttttttatac aattttcatg ggtagcccgc ctacccttat
      121 tattttttgc caattttgag gagggaacgc atgaaaacac gttgttacga tggtaaaaaa
      181 tggcaatatg aatttaagta tgaaggaaaa agataccgta agaaaggttt tagaacaaag
      241 cgtgaagcta attctgctgg actagacaag ttaaatgagt taagaagtgg ttttaatata
      301 gataactata taactcttga agaatacttc gaaaattgga ttaaaacgta taaacaacct
      361 gttgttaaag aaaataccta ccgtcattat agaaatgcat tacaacatat acaaaaacat
      421 aaaataggta aaatggagtt atcaaagata aatagacaag tttatcagaa attcataaac
      481 gattattcaa aagaacacgc aaaagaaact ataagaaaaa caaacggtgc tattcggtca
      541 gctttagatg acgcattata tgatgggctt atttttaaaa atcccgctta taaagttaat
      601 tataaagccg gaaaacctac gaagtcagaa caagaaaaat tcatctcggt aactgaatat
      661 gaaatactaa aagatcacgt cagaaagaag agaactcgtt catcattagc gctattcata
      721 atgatttgta cgggttgtcg tgtcagtggt gcaagaaata taaagattga gcatatcaac
      781 caagtgaaaa acactatatt tattgacgag cgaaaaaccg atacttcccc tagatatatc
      841 agtatcgcta aatctgatat gaaacacatt atggacgtca taagtacatt tgcaattagc
      901 tatgatggtt acattttcaa agaagccgga tctataatta accttcatgc tatcaataat
      961 gctttgaaat cagcctgtag agtcaataat atatcaatta ttacatcgca cgcattaaga
     1021 cacactcatt gttcttattt actagcaaaa ggtgtatcta tacattacat ttctaaaaga
     1081 ttaggtcata aaaatatagc aataactaca tccgtgtatt ctcatttgtt agaagaaaaa
     1141 tttaatgaag aggacaaaaa aacaactaaa attttagaaa gtatgtaatt tagggaccca
     1201 ttagggactc caaacccaat aaatactgtt gttacaaggt ttctatgtat ccaaactggg
     1261 ggcaatataa acgcgctgat ttaatcggac aatcttctta tattaaaaat aatgatgtcg
     1321 taatattcaa tgaagcattt gataatggtg catcagacaa attattaagt aatgtgaaaa
     1381 aagaatatcc ttatcaaaca cctgtactcg gccgttctca atcagggtgg gacaaaactg
     1441 aaggtagcta ctcatcaact gttgcagaag atggtggcgt agcgattgta agtaaatatc
     1501 ctattaaaga aaaaatccag catgttttca aaagcggttg tggattcgat aatgatagca
     1561 acaaaggctt tgtttataca aaaatagaga aaaatggtaa gaacgttcac gttatcggta
     1621 cacatacaca atctgaagat tcacgttgtg gtgctggaca tgatcgaaaa attagagctg
     1681 aacaaatgaa agaaatcagt gactttgtta aaaagaaaaa tatccctaaa gatgaaacgg
     1741 tatatatagg tggcgacctt aatgtcaata aaggcactcc agagttcaaa gatatgctta
     1801 aaaacttgaa tgtaaatgat gttctatatg caggtcataa tagcacatgg gaccctcaat
     1861 caaattcaat tgcgaaatat aattacccta atggtaaacc agaacattta gactatatat
     1921 ttacagataa agatcataaa caaccaaaac aattagtcaa tgaagttgtg actgaaaaac
     1981 ctaagccatg ggatgtatat gcgttcccat attactacgt ttacaatgat ttttcagatc
     2041 attacccaat caaagcctat agtaaatagt gctcaactaa ctaataactt gcttcgttct
     2101 aaaaggacga agcgagttat attgttaaaa tttgaattg
//
LOCUS       DAKTIO010000268         2120 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.276, whole genome shotgun sequence.
ACCESSION   DAKTIO010000268 DAKTIO010000000
VERSION     DAKTIO010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.276"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..67
                     /locus_tag="O0293_002736"
     CDS             <1..67
                     /locus_tag="O0293_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCY0281553.1"
                     /translation="FLVFFYALDLGITALKNLLFR"
     gene            80..628
                     /gene="nusG"
                     /locus_tag="O0293_002737"
     CDS             80..628
                     /gene="nusG"
                     /locus_tag="O0293_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCY0281554.1"
                     /translation="MSEEVGAKRWYAVHTYSGYENKVKKNLEKRVESMNMTEQIFRVV
                     IPEEEETQVKDGKAKTTVKKTFPGYVLVELIMTDESWYVVRNTPGVTGFVGSAGAGSK
                     PNPLLPEEVRFILKQMGLKEKTIDVELEVGEQVRIKSGPFANQVGEVQEIETDKFKLT
                     VLVDMFGRETPVEVEFDQIEKL"
     gene            809..1231
                     /gene="rplK"
                     /locus_tag="O0293_002738"
     CDS             809..1231
                     /gene="rplK"
                     /locus_tag="O0293_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012664221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCY0281555.1"
                     /translation="MAKKVDKVVKLQIPAGKANPAPPVGPALGQAGVNIMGFCKEFNA
                     RTQDQAGLIIPVEISVYEDRSFTFITKTPPAPVLLKKAAGIEKGSGEPNKTKVATVTK
                     DQVREIANSKMQDLNAADEEAAMRIIEGTARSMGIVVE"
     gene            1439..>2120
                     /gene="rplA"
                     /locus_tag="O0293_002739"
     CDS             1439..>2120
                     /gene="rplA"
                     /locus_tag="O0293_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCY0281556.1"
                     /translation="MAKKGKKYQEAASKVDRTQHYSVEEAIKLAKETSIANFDASVEV
                     AFRLGIDTRKNDQQIRGAVVLPNGTGKSQSVLVFAKGDKIAEAEAAGADYVGEAEYVQ
                     KIQQGWFDFDVVVATPDMMGEVGKLGRVLGPKGLMPNPKTGTVTMDVKKAVEEIKAGK
                     VEYRAEKAGIVHASIGKVSFTDEQLIENFNTLQDVLAKAKPSSAKGTYFKSVAVTTTM
                     GPGVKIDTA"
BASE COUNT          781 a          316 c          439 g          584 t
ORIGIN      
        1 cttcttagtc tttttctatg ccttagattt aggaattaca gcattgaaaa atttattatt
       61 tcgttagagg agtgaagaca tgtctgaaga agttggcgca aagcgttggt atgcagtgca
      121 tacatattct ggatatgaaa ataaagttaa aaagaattta gaaaaaagag tagaatctat
      181 gaatatgact gaacaaatct ttagagtagt cataccggaa gaagaagaaa ctcaagtaaa
      241 agatggcaaa gctaaaacga ctgttaaaaa aacattccct ggatatgttt tagtggaatt
      301 aatcatgaca gatgaatcat ggtatgtggt aagaaataca ccaggcgtta ctggttttgt
      361 aggttctgca ggtgcagggt ctaagccaaa tccattgtta ccagaagaag ttcgcttcat
      421 cttaaaacaa atgggtctta aagaaaagac tatcgatgtt gaactcgaag ttggcgagca
      481 agttcgtatt aaatcaggtc catttgcgaa tcaagttggt gaagttcaag aaattgaaac
      541 agataagttt aagctaacag tattagtaga tatgtttggc cgagaaacac cagtagaagt
      601 tgaattcgat caaatagaaa agctttaatt aacaattaaa gttattaaac taaccaaaag
      661 ataaaaaaga gtattgattt tttaattaga aaagtgttaa aattatgtgg tcgcgctttt
      721 agagcgccca tttcgtcacg aaatgttaag agtgggaggg caaaactgag ccctgtgacc
      781 acatcacgat atcaaggagg tgcacatcgt ggctaaaaaa gtagataaag ttgttaaatt
      841 acaaattcct gcaggtaaag cgaatccagc accaccagtt ggtccagcat taggtcaagc
      901 aggtgtgaac atcatgggat tctgtaaaga gttcaatgca cgtactcaag atcaagcagg
      961 tttaattatt ccggtagaaa tcagtgttta tgaagatcgt tcatttacat ttattacaaa
     1021 aactccaccg gctccagtat tacttaaaaa agcagctggt attgaaaaag gttcaggcga
     1081 accaaacaaa actaaagttg ctacagtaac taaagatcaa gtacgcgaaa ttgctaacag
     1141 caaaatgcaa gacttaaacg ctgctgacga agaagcagct atgcgtatta tcgaaggtac
     1201 tgcacgtagt atgggtatcg ttgtagaata attttacgaa tattaaattt gattacatga
     1261 tttaaacgat gaagcagata acagagataa taatgatgaa ttataaatat aatctgaatg
     1321 actagattaa tgattgattt attcataaga ttaattcttc tgttgtctgc tcttaacttg
     1381 catatagcaa gtaatgtggg aggaaattcc gctaaaacca ctaaaggagg aactataaat
     1441 ggctaaaaaa ggtaaaaagt atcaagaagc agctagtaaa gttgaccgta ctcagcacta
     1501 cagtgttgaa gaagcaatta aattagctaa agaaacaagc attgctaact ttgacgcttc
     1561 tgttgaagtt gcattccgtt taggaattga tacacgtaaa aatgaccaac aaatccgtgg
     1621 tgcagttgta ttaccaaacg gaactggtaa atcacaaagt gtattagtat tcgctaaagg
     1681 tgacaaaatt gctgaagctg aagcagcagg tgctgactat gtaggtgaag cagaatacgt
     1741 tcaaaaaatc caacaaggtt ggttcgactt cgatgtagta gttgctacac cagacatgat
     1801 gggtgaagtt ggtaaattag gtcgtgtatt aggaccaaaa ggtttaatgc caaaccctaa
     1861 aactggaact gtaacaatgg atgttaaaaa agctgttgaa gaaatcaaag ctggtaaagt
     1921 agaatatcgt gctgaaaaag ctggtatcgt acatgcatca attggtaaag tttcatttac
     1981 tgatgaacaa ttaattgaaa acttcaatac tttacaagat gtattagcta aagctaaacc
     2041 atcatctgct aaaggtacat acttcaaatc tgttgctgta actacaacaa tgggtcctgg
     2101 agttaaaatt gatactgcaa
//
LOCUS       DAKTIO010000269         2113 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.277, whole genome shotgun sequence.
ACCESSION   DAKTIO010000269 DAKTIO010000000
VERSION     DAKTIO010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.277"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..192
                     /locus_tag="O0293_002740"
     CDS             <1..192
                     /locus_tag="O0293_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0281557.1"
                     /translation="VLFSYFFCGGLSIMFLDVLISLSIQNIIVFIAWVIMTMLFFFIN
                     MSSIIDKKIHVIYLRSYKY"
     gene            280..897
                     /locus_tag="O0293_002741"
     CDS             280..897
                     /locus_tag="O0293_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0281558.1"
                     /translation="MLCETEIINKNPKYRVIKYDDEYLMVDVIRTWLVYFFPFINWFI
                     PKRCAKISREEYEKLNTVKPVKNKNFWPVVGGTILLGATSRKYIHLLNIQLEKRSVIF
                     ICFVVFLCILIFFVLLNRKLKLKVFDNKKEEQKIILVPTLKNAVLILYGYLLIGGMSI
                     LALSMLLTLENQNLITFIAWGMGLMLFFLMNITLIVNKTVKVIKR"
     gene            972..1601
                     /locus_tag="O0293_002742"
     CDS             972..1601
                     /locus_tag="O0293_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HCY0281559.1"
                     /translation="MLCESRVINQNPKYRIIKYNNEYFMVDLVSTWIAYFLPMINWFI
                     PKKYAKISREEFESLNIVKPAKNNTFWPVAGFAVLLTTLTRKYIYLLNIHLEKEIVIL
                     TCCMILLGVFALFIYINTKLKLHIFDKNKSNNEKIILIPTFKNICLSLFAYILFGGLS
                     TMALSMLVTSSPQNMIEFLALIGMTACFFLLNMSSVLDKKIHVILKTNK"
     gene            1658..1774
                     /locus_tag="O0293_002743"
     CDS             1658..1774
                     /locus_tag="O0293_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281560.1"
                     /translation="MILNRGAILEQGNKTAKVIKSELITNYIMLATLQIKNN"
     gene            1797..>2113
                     /locus_tag="O0293_002744"
     CDS             1797..>2113
                     /locus_tag="O0293_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0281561.1"
                     /translation="MLCESKIINKNPKYRIIKYNDEYLMIDIISTWISLFFPFINWFI
                     PKRYVKISREEFENLNIVKPAKKNVFWPVAGSSALLGVALRKYTHLLDIQLDKKLVIA
                     ICC"
BASE COUNT          781 a          244 c          328 g          760 t
ORIGIN      
        1 gtactatttt cttatttttt ctgtggtgga ttatcaatca tgttcttaga tgttttgatt
       61 agtttatcta tccaaaatat tatagtattt attgcttggg ttattatgac aatgctattt
      121 ttctttataa atatgtcttc aataatagac aaaaagattc atgttatata tttaaggtca
      181 tataaatatt aattttgata caaaagggca caagtgtttt gagaaacaga aacgataata
      241 tagctgtgtg aatttgttat taataggaag tgataattat tgctttgtga aaccgaaatt
      301 atcaataaaa atccgaaata cagagttatt aagtatgatg atgagtattt aatggtcgat
      361 gtaataagaa cttggcttgt atactttttt ccatttatta attggttcat tccaaaaagg
      421 tgcgccaaaa ttagtagaga agagtatgaa aaactgaaca ctgttaagcc agttaaaaat
      481 aaaaattttt ggcctgttgt aggaggaaca atcctactag gtgctacttc cagaaagtac
      541 atacacttac ttaatattca attagaaaaa agatcggtta tatttatatg cttcgttgta
      601 tttctatgta ttttaatctt ttttgtgctt ctgaatcgaa aattaaaatt gaaagttttc
      661 gataataaaa aagaagaaca aaaaataatt ttagtaccaa cattaaaaaa tgctgttctt
      721 atattatatg gctatttgtt gattggaggc atgtcaatat tagctttaag catgttgctg
      781 acattagaaa accagaattt aataactttt atagcttggg gtatggggtt aatgttgttt
      841 ttcttgatga atataacact cattgtcaat aaaacagtca aagttataaa aaggtgaata
      901 aataactaat tatattgtat tggccgttct tcaaataaaa aataatttga ataaggtgga
      961 agtgataaac agtgctttgc gaatctagag tcattaatca aaaccctaaa tatagaatta
     1021 ttaaatacaa taatgaatat ttcatggttg atttagtaag tacttggatt gcttattttt
     1081 tgcccatgat taattggttt attcccaaaa agtacgcgaa aattagtaga gaagaatttg
     1141 aaagtttaaa tattgtcaaa cccgctaaaa ataatacttt ctggcctgtt gcaggatttg
     1201 cagtgttatt aacaacctta acaagaaaat atatctattt gcttaacatc catttagaaa
     1261 aagaaatagt tatattaaca tgctgtatga tacttctagg tgttttcgca ttgtttatat
     1321 atataaatac aaaattgaag ttacatattt ttgataaaaa taaaagtaat aacgaaaaga
     1381 tcatattaat acctacattt aaaaatattt gtttatcctt atttgcttat atattatttg
     1441 gtggattgtc aacaatggct ctgagtatgt tagtaacttc atcccctcaa aatatgatag
     1501 aatttcttgc tttaattggc atgactgcat gcttctttct actgaatatg tcatcggttc
     1561 tagataaaaa aattcatgtt attttaaaaa caaataagta gtaaaattga ttaacttagg
     1621 tagtatcgga tacttaaatg ttggttcata aaaagcaatg attttaaatc gaggagctat
     1681 cttagaacag ggaaataaaa cagccaaagt tataaaaagt gaattaataa ctaattatat
     1741 tatgttagcc acgcttcaaa taaaaaataa ttagaataag gtgggattga taatcaatgc
     1801 tatgcgaatc taaaatcatc aataaaaacc ccaaatatag aattattaaa tataatgatg
     1861 agtacttaat gatcgatata ataagcactt ggattagttt attttttcct tttattaatt
     1921 ggttcatccc caaaagatac gtcaaaatta gtagagaaga gtttgaaaat ttaaatattg
     1981 tgaaacctgc taaaaagaat gttttttggc cagttgcagg tagctctgct ttgctgggag
     2041 ttgcattaag aaaatataca catttacttg acattcaact tgataaaaaa ttagttattg
     2101 ccatatgttg cat
//
LOCUS       DAKTIO010000270         2096 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.278, whole genome shotgun sequence.
ACCESSION   DAKTIO010000270 DAKTIO010000000
VERSION     DAKTIO010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.278"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            198..425
                     /locus_tag="O0293_002745"
     CDS             198..425
                     /locus_tag="O0293_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281562.1"
                     /translation="MTHLTKVLDTLTGICVVLLFSKYFVAYANMVFDWNLRWYLLENI
                     PHLPIILFILMFIFGVPSEMIKDRQRKNNGV"
     gene            complement(730..1677)
                     /locus_tag="O0293_002746"
     CDS             complement(730..1677)
                     /locus_tag="O0293_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HCY0281563.1"
                     /translation="MRLQKAPLVTSGLVLGLLGLGNLLKDLSLTLNAVCGIFAFLIWI
                     HLLCTMIKYFNNVKEQLNSPLVSSVFTTFFMSGFLGTTYLNTFFSNITFINSLITPIW
                     ILCLVGIMTHMIIFSIKYLKDFSLKNVYPSWTVLFIGIAIAGLTAPVSGYFFIGQLTV
                     IYGFVATCIVLPIVFKRLKAFPLQTSIKPNTSTICAPFSLVAAAYVIAFPKANAFIVI
                     IFLLLAQIFYFYIIIQLPKLLKEPFSPVFSAFTFPLVISATALKNSLPVLMFPDIWKG
                     LLFIEVLLATVIVLRVFIGYLHFFLKKEKQDKFLRNASQ"
     gene            1926..>2096
                     /locus_tag="O0293_002747"
     CDS             1926..>2096
                     /locus_tag="O0293_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCY0281564.1"
                     /translation="MRNQIQKLLDSDLSSLHISKQTGVPQSTIHRMRKNERSLDNMSL
                     KNAELLYKFANSI"
BASE COUNT          796 a          258 c          353 g          689 t
ORIGIN      
        1 gacggtaatg ataacactag taagtaattg atacaatgct ctatttaata atgatatttt
       61 taaaatttgt ttttaatgta atgttagatc tatggtatat tatatttatc gtggtaaata
      121 tgtatttgct gtaaacagtt tttgtaacac atgatacata tggttgtgaa aatagcatat
      181 ataaaggatg gctataaatg acacatttga caaaggtttt agatacacta actggaatat
      241 gcgtagtatt attatttagt aaatattttg tggcgtatgc aaatatggtg tttgattgga
      301 atttaagatg gtatttgcta gaaaacatac cacatttgcc aattatatta tttattctga
      361 tgtttatttt cggagtacct tctgaaatga taaaagatag gcaaaggaaa aataacggtg
      421 tttaatttat cgatatttag aggtgataaa atttgtcaac tattacaaag actaaaataa
      481 aaatctttat taattatttt attggtatat tagccttggc gtttgctgct tatatattat
      541 ttaaataaag tttagagtga aacgtgttta tgaactagac gttctagtat agttactata
      601 gctttagtca gaaggtatca ttgataagat catattaaat caaagaggca ttgatataca
      661 ctaaaaagag gcaagattac ctgcctcttt ttagttatta aatatacgtg ttaattcttg
      721 gtaatagtgt tactgagacg cattacgaag aaatttatct tgtttttcct tttttaaaaa
      781 gaagtgaaga tatcctataa agactctaag tactattaca gtggctaata acacttcgat
      841 aaacaaaaga cctttccaaa tgtctggaaa cataagtaca ggcaaactgt tctttaaagc
      901 agttgctgag attactaaag ggaatgtgaa agctgaaaat acgggcgaaa aaggttcttt
      961 tagtaattta ggcaattgta taatgatata aaaataaaat atttgagcta atagtaaaaa
     1021 tataattacg ataaaagcat tcgccttagg aaaagctata acatatgctg cagcgactaa
     1081 agaaaatggt gcacaaattg tcgatgtgtt cggtttgatt gacgtctgca atggaaatgc
     1141 ttttaatcgc ttgaaaacta taggtaagac aatacaagta gctacaaagc catatattac
     1201 tgttaattga cctatgaaaa aatatccgct aacgggtgcc gtcaatcctg cgatagcaat
     1261 accaataaaa agtacagtcc acgaaggata aacattttta agtgaaaaat cttttaaata
     1321 ttttattgaa aaaataatca tatgcgtcat aattcccaca aggcataaaa tccaaatagg
     1381 cgttattaag ctattgataa aagttatgtt actaaaaaat gtatttaaat aagtagtacc
     1441 taaaaagcca gacatgaaaa atgttgtgaa cactgatgaa actagaggac tgtttaattg
     1501 ttctttcaca ttattaaaat atttgatcat agtacataaa aggtgaatcc aaatcaagaa
     1561 agcaaagatt ccgcaaacag cgtttaaagt aagagataag tcttttaata gattacccag
     1621 gcctaataat cctaagacta gtcctgacgt tactagaggt gctttttgaa gtctcatgat
     1681 ttacaacctt tcttatgtga ttttcttcac taattatatc atgatagcta tggccaatta
     1741 ataagaagag tgtgtactat tacgttatta gagtatgtat attggattga tagacacata
     1801 attgacattt aaatctcaaa attaatgata tttgaggtat gctttgcaac ctaaatattg
     1861 gaatatgtgg aaaagtaatt atttaattta aatgatgatt gaagatttac aagggggtgt
     1921 acaaaatgag aaatcaaatt caaaaactat tagacagtga tttgagcagt ttacatatat
     1981 cgaaacaaac aggagttcca caaagcacaa tacacagaat gagaaaaaat gaaagatcat
     2041 tagacaatat gtcattgaaa aacgctgaac tactttataa atttgccaat agtata
//
LOCUS       DAKTIO010000271         2035 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.279, whole genome shotgun sequence.
ACCESSION   DAKTIO010000271 DAKTIO010000000
VERSION     DAKTIO010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.279"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            115..1644
                     /gene="aur"
                     /locus_tag="O0293_002748"
     CDS             115..1644
                     /gene="aur"
                     /locus_tag="O0293_002748"
                     /EC_number="3.4.24.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloproteinase aureolysin"
                     /protein_id="HCY0281565.1"
                     /translation="MRKFSRYAFTSMAALTLLSTLSPAALAIDSKNKPANSDIKFEVT
                     QKSDAVKALKELPKSENVKNIYQDYAVTDVKTDKKGFTHYTLQPSVDGVHAPDKEVKV
                     HADKSGKVVLINGDTDAKKVKPTNKVTLSKDDAADKAFKAVKIDKNKAKNLKDKVIKE
                     NKVEIDGDSNKYVYNVELITVTPEISHWKVKIDAQTGEILEKMNLVKEAAETGKGKGV
                     LGDTKDININSIDGGFSLEDLTHQGKLSAFSFNDQTGQATLITNEDENFVKDEQRAGV
                     DANYYAKQTYDYYKDTFGRESYDNQGSPIVSLTHVNNYGGQDNRNNAAWIGDKMIYGD
                     GDGRTFTSLSGANDVVAHELTHGVTQETANLEYKDQSGALNESFSDVFGYFVDDEDFL
                     MGEDVYTPGKEGDALRSMSNPEQFGQPAHMKDYVFTEKDNGGVHTNSGIPNKAAYNVI
                     QAIGKSKSEQIYYRALTEYLTSNSNFKDCKDALYQAAKDLYDEQTAEQVYEAWNEVGV
                     E"
BASE COUNT          811 a          279 c          391 g          554 t
ORIGIN      
        1 aaaatcaacg aacaaaaagt atagaaataa atagaaataa tcatttactt ttctgaaaaa
       61 ttaaattaat attttattta taagctgttt ttaagatttc aggaggaatg aaatgtgagg
      121 aaattttcaa gatatgcatt tacaagtatg gcagcattaa ccttgttgag cactttatca
      181 ccagcagcat tagcgattga ttcaaaaaat aaaccagcta attctgatat taaatttgag
      241 gtgactcaaa agagtgatgc ggtcaaagca ttaaaagaat tgcctaaatc cgaaaatgta
      301 aaaaatattt atcaagatta cgctgttact gatgtaaaaa ctgataaaaa aggatttacg
      361 cattatacat tgcaaccgag tgttgatggt gttcatgcac ctgacaaaga agtgaaagta
      421 cacgcagaca aatcaggaaa agtcgtttta atcaatgggg atactgatgc gaagaaagta
      481 aagccaacga ataaagtgac attaagtaaa gatgacgcag ccgacaaagc atttaaagca
      541 gttaagattg ataagaataa agcgaaaaat cttaaagata aagtcattaa agaaaacaaa
      601 gttgaaatcg atggtgacag taataaatac gtttataatg ttgagttaat tacagtgaca
      661 ccagaaattt cacattggaa agttaaaatt gatgctcaaa ctggcgaaat tttagaaaaa
      721 atgaacttag ttaaagaagc tgcagaaact ggtaaaggaa aaggtgtact tggcgataca
      781 aaagatatca atatcaatag tattgacggt ggatttagcc tagaagattt aacgcatcaa
      841 ggtaaattat cagcatttag ctttaatgat caaacaggtc aagcaacatt gattactaat
      901 gaagatgaaa acttcgtaaa agatgagcaa cgtgctggcg tagatgcaaa ttattacgct
      961 aaacaaacat atgattatta caaagacaca tttggtcgtg aatcatatga caaccaaggt
     1021 agtccaattg tttcattaac gcatgttaat aactacggtg gtcaagataa cagaaataat
     1081 gccgcatgga tcggtgacaa aatgatctat ggtgatggtg atggtcgcac attcacaagt
     1141 ttatcgggtg caaatgacgt agtagcacac gaattaacac acggtgtgac acaagagaca
     1201 gcgaacttag aatataagga ccagtcaggc gctctaaatg aaagcttttc agatgttttt
     1261 ggatactttg tagatgacga ggatttctta atgggtgaag atgtctacac acctggaaaa
     1321 gagggagacg ctttacgcag catgtcaaac ccagaacaat ttggtcaacc agctcatatg
     1381 aaagactatg tattcactga aaaagataat ggtggcgtac atacgaattc tggaattcca
     1441 aataaagcag cttataacgt gattcaagca atagggaaat ctaaatcaga acaaatttac
     1501 taccgagcat taacggaata cttaacaagt aattcaaact tcaaagattg taaagatgca
     1561 ttataccaag cggctaaaga tttatatgac gagcaaacag ctgaacaggt gtatgaagca
     1621 tggaatgaag taggcgtgga gtaaaaatat ataaacaaga agaagtaatg ttaaacactt
     1681 ataaataatt aaattttaaa tacagcttat atatggggta tacctacatt gataattgac
     1741 tttgtaggta taccctctct ttgtgattag atgagaggag tgtgagggtt gtctgccgaa
     1801 agactactcg gcagtctaaa atcattacaa gtagtagata tgtgataatt aaatgctgac
     1861 ttagaataca aaattcattt taaaagttgt cacaaaaaat ttacatgtat ttttattata
     1921 ttttgcaaaa caaagtgtta aattataaat gaaacatgca tgaatttatt ttttaataca
     1981 agaaacgtaa ctaccaaagg agtttacaat atgaagaaaa gtaaacgatt agaaa
//
LOCUS       DAKTIO010000272         2025 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.280, whole genome shotgun sequence.
ACCESSION   DAKTIO010000272 DAKTIO010000000
VERSION     DAKTIO010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.280"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            45..1190
                     /locus_tag="O0293_002749"
     CDS             45..1190
                     /locus_tag="O0293_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide dioxygenase"
                     /protein_id="HCY0281566.1"
                     /translation="MLTEQEKDIIKQTVPLLKEKGTEITSIFYPKMFKAHPELLNMFN
                     QTNQKRGMQSSALAQAVMAAAVNIDNLSVIKPVIMPVAYKHCALQVYAEHYPIVGKNL
                     LKAIQDVTGLEENDPVIQAWAKAYGVIADVFIQIEKEIYDQMMWIGFKPFKITNIKQE
                     SEDIKSFTVETEEYDFSEFTPGQYITVDVSSDKLPYRAKRHYSIVSGEKNHLTFGVKR
                     DVTTEHEGEVSTILHDEIKEGDMINLAAPVGGFVLENTTEPQLFLGSGIGVTPLVAMY
                     EAASAKGLDTQMVQVAENEQHLPFKDNFNSIASHHDNAKLYTHLKDKQGYIGAEELQV
                     FLANKPEIYICGGTKFLQSMIEALKSLNYDMDRVHYETFIPRLSVAV"
     gene            1216..1389
                     /locus_tag="O0293_002750"
     CDS             1216..1389
                     /locus_tag="O0293_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281567.1"
                     /translation="MKLNQRYVKVFALYFVSIVTANIIVKNNNLIKTLIQTIAGYTVF
                     AVGLKYLTKRKNK"
     gene            1546..1902
                     /locus_tag="O0293_002751"
     CDS             1546..1902
                     /locus_tag="O0293_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCY0281568.1"
                     /translation="MTVDIGRIYDNKDNTDAIRILVDRVWPRGISKRTANLDYWLKDI
                     APSTELRQWFQHDPKLFGAFKEKYEKELRDQDAQKDAFEKLKDIVNQHNHVLLLYAAK
                     DTKHNQAVVLQQLLNT"
BASE COUNT          742 a          326 c          346 g          611 t
ORIGIN      
        1 ttttagtaga ataatcaaaa tatacagatt aaaggaggag tatcatgctt acagaacaag
       61 agaaagacat tatcaaacaa acggtgcctt tacttaaaga gaaagggaca gaaattacgt
      121 caatctttta tccaaaaatg tttaaagcgc atcctgaact tttaaacatg tttaatcaaa
      181 cgaaccaaaa acgaggcatg caatcttcag cattagcaca agctgtaatg gccgcagcgg
      241 ttaatatcga taacttaagt gttattaaac cagtcattat gccagtcgca tataaacact
      301 gcgcactaca agtttatgct gaacattatc caattgtggg gaaaaattta ttaaaagcca
      361 ttcaagacgt gacaggatta gaagaaaatg accctgtcat tcaagcttgg gcaaaagcat
      421 atggcgttat tgcggatgtg ttcatccaaa ttgaaaaaga aatttatgat caaatgatgt
      481 ggattggttt taaaccattc aaaattacca atattaaaca agaatctgaa gacattaaat
      541 catttacagt tgaaactgaa gaatatgact ttagtgaatt tacaccaggc caatacatca
      601 cagttgatgt ttctagtgat aaacttccat atagagctaa acgtcactat tctatcgtat
      661 caggtgaaaa aaaccattta acttttggcg ttaaacgtga tgtcacaaca gaacatgaag
      721 gcgaagtttc aacaatttta cacgatgaga ttaaagaagg cgatatgatt aatttagctg
      781 cgcctgtagg tggattcgta ttagagaata cgactgaacc acaacttttc ttaggttcag
      841 gtattggtgt tacaccttta gtagctatgt atgaagctgc ctctgccaaa ggtttagata
      901 cacagatggt tcaagttgct gaaaatgaac aacatttacc tttcaaagac aacttcaaca
      961 gtatcgcaag ccatcatgac aacgctaaat tatatacaca cttaaaagat aaacaaggct
     1021 atattggtgc tgaagaatta caagtatttt tagcaaataa acctgaaatt tatatctgtg
     1081 gtggtacaaa attcttacaa tctatgatcg aagcacttaa atctttaaat tacgatatgg
     1141 atcgcgtaca ctacgaaaca tttattccaa gactaagcgt tgcagtataa ctgagaaaag
     1201 aggtttagtg ctcatatgaa acttaatcaa cgttacgtaa aagtatttgc attatatttc
     1261 gtaagtattg ttactgcaaa tattattgtt aaaaataata atttaattaa aacattgata
     1321 caaaccatag ccgggtacac ggtctttgca gttggtttga agtatttaac taaacgtaaa
     1381 aataaatgac atctatacca aaacagctat gactttactt tgtcatggct gttttttaat
     1441 tgggagtagg acagagatga tattttcgca aaatttattt ggtcgtccca ccacaacatg
     1501 cattgatgta tgctcactga atttcataag aaaggagttc acaagatgac cgtagatatt
     1561 ggacggattt atgacaataa agataatacc gacgctattc gtatcctagt cgatagagtc
     1621 tggccgagag gtatttcgaa aagaactgct aacctagatt attggttaaa agacattgcc
     1681 ccttctactg agttgcgaca atggttccaa catgatccta aactttttgg agcttttaaa
     1741 gaaaaatatg aaaaagaatt acgtgatcag gatgcgcaaa aagatgcttt tgaaaaatta
     1801 aaggatattg taaatcagca taatcatgtt ctattgttat atgcagcaaa agatactaaa
     1861 cataaccaag ctgtagtact acagcagttg ctcaatactt agttatatat gtttatcgtg
     1921 aatcactata aatgtcgcta acttcatttc taatatttat ttttaaaaag cataattact
     1981 acaattaatt gaactttaat aattactaaa cttgaacaac atttt
//
LOCUS       DAKTIO010000273         1987 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.282, whole genome shotgun sequence.
ACCESSION   DAKTIO010000273 DAKTIO010000000
VERSION     DAKTIO010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.282"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..165)
                     /locus_tag="O0293_002752"
     CDS             complement(<1..165)
                     /locus_tag="O0293_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281569.1"
                     /translation="MDYAHLNLEHFFARNDDLDVIRDRADFVMINNFTNEMMYRDGQI
                     EGTIDLNQYYY"
     gene            364..834
                     /gene="sigS"
                     /locus_tag="O0293_002753"
     CDS             364..834
                     /gene="sigS"
                     /locus_tag="O0293_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigS"
                     /protein_id="HCY0281570.1"
                     /translation="MKFNDVYNKHHKIIHHLLKKYNISYNYDEYYQLLLIKMWQLSQI
                     YKPSSKQSLSSFLFTRLNFYLIDLFRQQNQLKDVILCENNSPTLTEQPTYFNEHDLRL
                     QDIFKLLNQRERLWLKLYLEGYKQFEIAEIMSLSLSTIKLIKMSVKRKCQHNFN"
     gene            947..1390
                     /locus_tag="O0293_002754"
     CDS             947..1390
                     /locus_tag="O0293_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HCY0281571.1"
                     /translation="MQDNSTKYLLYIQTATSNHLETNCVFLHCDYILKVPINKLVSYY
                     AKLHLSSQSVLIETAKNILNINKLVPIYINPKTILFPLKHKRAPIQIYINAHYIVGMT
                     AIENSTLIHFQEGIQLEVDEPFSLVSKKCHESLALKHFIENTISN"
     gene            complement(1377..1820)
                     /locus_tag="O0293_002755"
     CDS             complement(1377..1820)
                     /locus_tag="O0293_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281572.1"
                     /translation="MNKASFDKKVKKQLWFLNKKEKQELDQRLSSISDDDSVNLNKPV
                     TFANAYLRQNVFRNKETKSYSMFVTLVVMMFAYVALLGLFLFGLITSLSGVQFFVSPK
                     VDLSTTVVILTIIGAILLMFASIYFIKIVTSYFTKKLLEIKFNSK"
BASE COUNT          737 a          327 c          222 g          701 t
ORIGIN      
        1 ataatagtac tgatttaaat caatcgtgcc ttcaatttga ccatcacgat acatcatttc
       61 attagtgaag ttatttatca tcacgaaatc agcgcgatct cttataacat ctaaatcgtc
      121 gtttcgtgca aaaaaatgtt ctaaatttaa atgtgcgtaa tccattgtta cctcccgatt
      181 acaaattttg ttctatctac tatttagcaa agtaataata catcatatca cattaaatta
      241 attacttatg tcttatattt aacacttaat ttagcgatta cgcaaatgaa tgatttttct
      301 aaatatttta ttctaacttt atccggaaat atttgttata cgtataactt ggagaatgac
      361 tacttgaaat ttaatgacgt atacaacaaa caccacaaaa tcatacacca tcttttaaaa
      421 aaatataata ttagctataa ttatgatgag tattatcaac tactcttgat aaaaatgtgg
      481 caattgagtc agatatataa accctcaagc aagcaatctt tatcctcttt tttattcact
      541 cgattaaatt tttaccttat cgatttattc agacaacaaa atcaattaaa agatgtcatt
      601 ttatgtgaga ataattcacc aacattaact gaacaaccaa catactttaa tgaacatgac
      661 cttcgtttac aagatatctt caagctttta aatcaaagag aaagactatg gctcaaacta
      721 taccttgaag gatacaagca atttgaaatt gctgaaatca tgtcattatc gctttcaacg
      781 attaaattaa ttaagatgtc cgttaagcgt aaatgccaac ataattttaa ttagtttatt
      841 gtgaaatttc ggtcgatgtg ttaatgatgt tgaaacaccc taacatcttc atataaattt
      901 attttaacca ttaaattaac aacttattaa acgaggagta ctatatttgc aagacaattc
      961 tactaaatat ctactttata tccaaactgc tacttcaaac catctcgaaa caaattgtgt
     1021 ctttttacat tgcgattaca ttcttaaagt tccaattaac aaactcgttt catattatgc
     1081 gaaattgcat ttatcatcac aaagtgtgct aattgagact gcaaaaaaca tactaaatat
     1141 taataaactg gttcctattt acatcaaccc aaaaactata ctttttccgt taaaacataa
     1201 acgtgcaccg atacaaattt atatcaatgc acattatatt gttggtatga ctgccataga
     1261 gaattcaaca ttaatacatt ttcaagaagg cattcagcta gaagttgatg agccattttc
     1321 tctagtttca aaaaaatgtc atgaaagttt agctttgaag cacttcattg aaaatactat
     1381 ttcgaattaa atttaatttc taaaagtttc ttagtaaagt atgatgtcac aatttttata
     1441 aaatatatgc tagcaaacat taataaaatc gctccaataa ttgttaaaat aacaacagtt
     1501 gtagataaat ctaccttagg actaacgaaa aattgtacgc cagatagact tgtaattagc
     1561 ccaaataaaa acaaacctaa taaagcaaca taagcaaaca tcatcactac taatgtaaca
     1621 aacatactat aactttttgt ttctttattt cgaaatacat tttgtcttaa gtaagcatta
     1681 gcaaaggtaa caggcttatt taagttaacg ctgtcatcat cagatataga agacaatcgt
     1741 tgatctaatt cttgtttctc cttcttgttc aaaaaccata attgcttttt aactttttta
     1801 tcaaatgatg ctttattcat aattaaggat ttctctcctt tataatattg acgtaatttt
     1861 aaacattagc gtcaattgaa agtgtatttt atatatttta cagaaatata tgtatcaaat
     1921 ctacaaaaat ccatattaca atttcattta tggtgatttg acgctaaaat acagtctgct
     1981 aattttt
//
LOCUS       DAKTIO010000274         1974 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.283, whole genome shotgun sequence.
ACCESSION   DAKTIO010000274 DAKTIO010000000
VERSION     DAKTIO010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.283"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..164
                     /locus_tag="O0293_002756"
     CDS             <1..164
                     /locus_tag="O0293_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF600 family protein"
                     /protein_id="HCY0281573.1"
                     /translation="FDYIDWINSEFGQVGRQNYYKYRKFGILPETEYEINKVKEIEQY
                     VKEQEEAEQ"
     gene            175..675
                     /locus_tag="O0293_002757"
     CDS             175..675
                     /locus_tag="O0293_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0281574.1"
                     /translation="MNFEEKLSEMYNKIANEINEMIPVEWEKVYVIAYVDDGGGQVIF
                     YYTKPRNDELYYYSSIVEDYNVLEEIFDDLWMELYRSFKKLRNIFKEESLEPWTSCEF
                     DFTKEDKLKVSFDYIDWKNTEFDQLGQENYYNYRKFGIIPEMEYEMEEVKQIEQYIKE
                     QEEAEQ"
     gene            686..1181
                     /locus_tag="O0293_002758"
                     /pseudo
     CDS             686..1181
                     /locus_tag="O0293_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498867.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
     gene            1192..1683
                     /locus_tag="O0293_002759"
     CDS             1192..1683
                     /locus_tag="O0293_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0281575.1"
                     /translation="MTFEEKLSEMYSEIANKIISMIPVEWEKVYAMAYIDEECGEVFY
                     NYTEPSSDELFYYTSVIKKYNLLKSSFMDSVYELHDQFEELREVFIEEGLEPWTSCEF
                     DFTKEDKLKVSFDYIDWINTEFDQLGRENYYMYKKFGVLPETEYEMEEVKEIEQYIKE
                     QEE"
     gene            1893..>1974
                     /locus_tag="O0293_002760"
     CDS             1893..>1974
                     /locus_tag="O0293_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 domain-containing protein"
                     /protein_id="HCY0281576.1"
                     /translation="MTTKENIDILRKPGAQALSLASLFMIL"
BASE COUNT          817 a          183 c          381 g          593 t
ORIGIN      
        1 catttgatta tattgattgg ataaattcag aatttggtca agtaggtcga caaaattact
       61 ataagtatag aaaatttgga attttaccag aaacggaata tgaaattaat aaagttaaag
      121 aaatcgagca atatgttaaa gagcaagaag aagctgaaca atagaggcga taacatgaat
      181 ttcgaagaaa aattaagtga aatgtacaat aaaattgcaa atgagatcaa tgaaatgata
      241 ccagtagagt gggaaaaggt atatgtaatt gcatatgtgg acgatggagg tgggcaagtt
      301 atattttatt ataccaagcc cagaaatgat gagctgtatt attattcgag tatcgttgaa
      361 gattataatg ttttagaaga aatctttgat gatttatgga tggaacttta tagatcattt
      421 aaaaaattaa gaaatatatt taaagaagaa agtcttgaac catggacatc atgtgaattt
      481 gactttacaa aagaagataa attaaaagtt tcatttgatt atattgattg gaagaataca
      541 gaatttgatc aattaggcca agaaaactac tataactata gaaaattcgg aattatacca
      601 gaaatggaat atgaaatgga agaggttaaa caaatcgagc aatatattaa agagcaagaa
      661 gaagctgaac aatagaggcg ataacatgat tttcgaagaa aaactaaatg aaatgtacaa
      721 cgagattgcg aataaaatta gtagcatgat accagtagaa tgggaaaagg tatatacaat
      781 ggcttatata gatgatggag gaggtgaagt attctttaat tatactaaac agcgatgaat
      841 tgaattatta caccgatata cctaaggagt ataacatttc tgtgcaagta tttgatgatt
      901 tatggatgga tttatatgat ttgtttgagg aattaagaaa tttatttaaa gaagaaggac
      961 tagaaccatg gacatcatgc gaatttgatt ttacaagaga aggtaaatta aaagtttcat
     1021 ttgattatat tgattggata aattcagaat ttggtcaagt aggtcgacaa aattactata
     1081 agtatagaaa atttggaatt ttaccagaaa cggaatatga aattaataaa gttaaagaaa
     1141 tcgagcaata tattaaagag caagatgaag ctgaactata ggggcgataa tatgactttc
     1201 gaagaaaaat taagtgaaat gtatagcgag attgcgaata agattattag catgataccg
     1261 gtagagtggg aaaaagtata tgcaatggca tatatcgatg aagaatgtgg agaagtgttt
     1321 tacaattata ctgaaccaag cagtgatgaa ttattttact atacaagtgt gataaagaag
     1381 tataatttat tgaaatcaag ctttatggat tcagtatatg agttgcatga tcaatttgag
     1441 gaactaagag aagtatttat agaagaaggt cttgaaccat ggacatcatg cgaatttgat
     1501 tttacaaaag aagataaatt aaaagtttca tttgattata ttgattggat aaatacagag
     1561 tttgatcaat taggccgtga aaattattat atgtacaaaa aatttggtgt tttaccagaa
     1621 acggaatatg aaatggagga ggttaaagaa atcgagcaat atattaaaga gcaagaagaa
     1681 tagacaatct taatgtaaga ttaaacaata aagctttgtt tagtcttttt agcgtttaag
     1741 taaaaaacaa tagagaccat aaagttgtca tcaaataata atataaagat aattttaggc
     1801 ttttatactt ttaatcgaaa agaatctaag tacttataac tttaaatact taatatgcta
     1861 ttacaaaatc aatgtatagg agataggtaa atatgactac taaggaaaat atagacattc
     1921 ttcgaaagcc aggtgcacaa gccttaagtt tagcatcatt atttatgata cttt
//
LOCUS       DAKTIO010000275         1958 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.284, whole genome shotgun sequence.
ACCESSION   DAKTIO010000275 DAKTIO010000000
VERSION     DAKTIO010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.284"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(409..894)
                     /gene="lnu(A)"
                     /locus_tag="O0293_002761"
     CDS             complement(409..894)
                     /gene="lnu(A)"
                     /locus_tag="O0293_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011645015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(A)"
                     /protein_id="HCY0281577.1"
                     /translation="MKNNNVTEKDLFYILDLFEHMKVTYWLDGGWGVDVLTGKQQREH
                     RDIDIDFDAQHTQKVIQKLEDIGYKIEVDWMPSRMELKHEEYGYLDIHPINLNDDGSI
                     TQANPEGGNYVFQNDWFSETNYKGRKIPCISKEAQLLFHSGYDLTEKDHFDIKNLKSI
                     T"
     gene            1147..>1958
                     /locus_tag="O0293_002762"
     CDS             1147..>1958
                     /locus_tag="O0293_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002499696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HCY0281578.1"
                     /translation="MKKYTEKKQRNQVFQNFIKRHIGENQMDLVENCNTFLSFVTNRR
                     MDKKKLYKSNPCKNRFCPMCAWRKARKDALGLSLMMQHIKQAEDKQFIFLTLTTPNVT
                     SEHLESEIKHYNQSFRRLSNRKHFKSIAKGYVRKLEITYNKKRDDYNPHFHVLIAVNK
                     SYFKDTKAYITQKEWLEMWRDVTGNSEITQVHIQKVKQNNNKELYEMAKYSGKDNDYL
                     INQKVFDAFYKALKGKQVLVYSGLFKDARKKLKNGELDYLKEVDPTEYIYQI"
BASE COUNT          726 a          268 c          304 g          660 t
ORIGIN      
        1 ttatttggct ctcatttgcg ttttaagagc caatataagt ttttgggtat aaaactatat
       61 aaataactct aatttttcaa aataccgcct taaaattaaa aataaaggta ttctgatttg
      121 taataaatta aaatataaaa aaagagccac catttaaaat ttaggcagct cttactcact
      181 ttttggatga taaaagtgga aaattaattc gagaaaaaac agaaaggata ttttaattat
      241 aacacggcat ttatgccgag aaaatttatt gatgttgaga agaaccctta actaaacttg
      301 tagacgaatg tcggtatagc gtgagctatt aaaccgacca ttcgacaagt tttgggattg
      361 ttaagggttc cgaggctcaa cgtcaataaa gcaattggaa taaagcaatt atgttattga
      421 ttttaaattt tttatatcaa aatggtcttt ttctgttaaa tcataaccag aatgaaaaag
      481 aagttgagct tcttttgaaa tacatggtat ttttcgacct ttgtaattag tttctgaaaa
      541 ccagtcattt tggaaaacat aattaccacc ttctgggttt gcttgggtaa ttgatccatc
      601 atcatttaga tttataggat gaatatctaa atacccatat tcttcatgct taagttccat
      661 acgtgaaggc atccaatcaa cttctatttt gtatcctata tcttctaatt tttgtataac
      721 tttttgagtg tgttgagcgt caaaatctat atctatatct ctgtgttctc tttgttgttt
      781 tccagttaat acatctaccc cccagccacc atctaaccaa taagttactt tcatgtgttc
      841 aaataaatct aaaatataaa ataaatcttt ttctgttaca ttattatttt tcattttatc
      901 cctcctaact tgggaatttg aaacaagttg aaaacgagtt tcttcttgtc ttgataataa
      961 gggtaactat tacaagctgt gatagttacc cttattccat tgatatgact ggttttaagc
     1021 acaaaaaaag ttgccttttc cagcatacta ttgtatcgtt ttaggtgtct aagctaaacg
     1081 aaaaatagaa aggagaaaaa gcaacgtctt ttcgtatttc aaacaaagtt gttttatgtg
     1141 aattatatga aaaagtatac tgagaaaaaa caaagaaatc aagtatttca aaactttatt
     1201 aaacgacata ttggggaaaa tcaaatggat ttagttgaga attgtaatac atttttgtct
     1261 tttgtgacga atagaagaat ggacaaaaag aaattatata aatctaatcc atgtaaaaat
     1321 cgtttttgtc cgatgtgtgc atggcgaaag gcgagaaaag atgcattagg tttgtcattg
     1381 atgatgcagc atattaagca agcagaagat aagcaattca tctttttaac acttacaacg
     1441 ccaaatgtaa caagtgaaca tttggaaagt gaaataaaac attataatca atcattcaga
     1501 cgattaagta atcgtaaaca ttttaagtct atagctaaag gttatgttag aaaattagaa
     1561 attacttata acaaaaagcg tgatgattat aacccacatt ttcatgtatt gattgccgtt
     1621 aataaatcat attttaaaga tactaaagct tatattactc aaaaagaatg gctagaaatg
     1681 tggcgtgatg tcacaggaaa ttctgaaatt acacaagtac atatacaaaa agttaagcaa
     1741 aataataata aagaacttta cgaaatggct aaatattctg gtaaggataa tgactattta
     1801 attaatcaaa aagtgttcga cgcattttat aaagcgttaa aaggaaaaca agttcttgtt
     1861 tattctggac tgtttaaaga cgcaagaaag aaattaaaaa atggagaatt agattactta
     1921 aaagaagttg atccgaccga atatatttat caaatttt
//
LOCUS       DAKTIO010000276         1950 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.285, whole genome shotgun sequence.
ACCESSION   DAKTIO010000276 DAKTIO010000000
VERSION     DAKTIO010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.285"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(134..1153)
                     /locus_tag="O0293_002763"
     CDS             complement(134..1153)
                     /locus_tag="O0293_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCY0281579.1"
                     /translation="MTGEQFTQIKRPVSRLTEKVLGWLCWVMLLVLTVITMFIALVSF
                     SNNTSIANLENTLNNNAFIQQLLAGNGYNTTQFVIWLQNGIWAIIVYFIVCLLISFLA
                     LISMNIRILSGFLFLISAIVTIPLVLLIVTLIIPILFFIIAMMLFIRKDKVEMVAPQY
                     YEEYNGPIYDYREPVYERPQPKDDYYDVPKYEKELDKSNTVYDQEQERDKYDQFPKRA
                     VESEYNHDERTEEEPSVLSRQAKYKQKSTEELGIEDDGYYAEPEVDPKELKAQQKREK
                     AEIKAKKKEKRKAYNQRMKERRKNQPSAVSQRRMNFEERRQIYNNDISEERNSSEVKD
                     KKEQE"
     gene            complement(1227..>1950)
                     /locus_tag="O0293_002764"
     CDS             complement(1227..>1950)
                     /locus_tag="O0293_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HCY0281580.1"
                     /translation="DSDYMNMSFDRGNKYSLPYFFGTVGILYNKEKYPNESFDSWKSL
                     YNPKFKNQILLVDGAREIIGMSLNKLGYNLNDRNSHHLKEAERDLTKLAPQVRGVVGD
                     EITMMLQQNEGNIAVVWSGVAAPLVQEGDKYNYVIPKEGSNLWFDNMVIPKTAQNKEG
                     AYKFMNFLLDAKNNKQNTEFVGYATPNKAARQLLPKEIKDDHRFYPTKKEQERLEVYK
                     DLGPEVLSEYNENFLNFKMSLK"
BASE COUNT          603 a          358 c          255 g          734 t
ORIGIN      
        1 gtaatgagaa atcgtcattt aaaattatga cacctaaaca ctttaattaa acgttatcga
       61 taattaacct catataaaaa cccagattaa taagcaaaag acctctgctt actaatctgg
      121 gtatcatcaa tatttattct tgctcttttt tgtccttaac ttcacttgaa ttgcgttctt
      181 cagaaatatc attgttgtaa atttgacgtc gctcttcaaa attcattcga cgttgactaa
      241 ctgcgctagg ttgatttttc ctacgttctt tcattcgttg gttataagct ttacgttttt
      301 cttttttctt agctttaatt tcagcttttt ctcgcttttg ttgtgctttc aattcttttg
      361 gatcaacttc aggttctgca taataaccat catcttcaat acctagttct tcagtacttt
      421 tttgtttgta tttagcctgt ctggataata ctgatggttc ttcctcagtg cgctcatcat
      481 gattatattc actttcaact gcacgtttag gaaattgatc atatttatct ctttcctgtt
      541 cttgatcata tacagtattt gatttatcca attctttttc atatttaggc acatcataat
      601 aatcatcttt cggttggggg cgctcataca caggttcacg ataatcataa atcggtccgt
      661 tatactcttc ataatattgt ggcgcaacca tttcaacttt atcttttctt ataaatagca
      721 tcatcgcaat aataaagaat aatatcggaa tgattaaagt aacgataagt aaaactaaag
      781 gaatcgttac aattgctgat attaagaata agaaacctga caagattctt atattcatag
      841 atatgagagc taaaaatgaa atcaacaaac aaacaataaa gtaaacgata atagcccata
      901 taccattttg taaccatatt acaaattgtg ttgtattata gccatttcca gctaataatt
      961 gctggataaa tgcattattg tttaatgtat tttcaagatt agcaatcgat gtgttattac
     1021 tgaatgaaac aagtgctata aacatcgtaa tgacagtaag cactaataac atcacccaac
     1081 ataaccaacc tagaactttt tcagttaatc tacttactgg acgtttaatt tgagtaaatt
     1141 gttctccagt cattcgttac aactccttat agtacttatc ccgttattat aactaaatat
     1201 acagtaaata actactattt atgattttat tttaatgaca ttttgaaatt caaaaagttt
     1261 tcattgtatt cacttaaaac ttcaggtcct aaatctttat aaacttcaag gcgttcttgc
     1321 tctttcttag tcggataaaa acgatggtcg tctttaatct ctttaggcaa caattgtcga
     1381 gcagccttgt ttggcgttgc atagcctacg aattctgtat tttgcttgtt atttttagca
     1441 tctaataaaa aattcataaa tttatatgca ccctctttat tttgtgccgt ttttggaatt
     1501 accatattgt cgaaccataa attcgatcct tctttaggaa taacataatt atatttatcc
     1561 ccttcttgca ctagaggtgc tgcaacacca ctccaaacaa ccgctatgtt accttcattt
     1621 tgttgaagca tcatggtaat ttcatcacct acgacacctc ttacttgtgg tgctagtttg
     1681 gttaaatctc gctctgcttc ttttaaatgg tgcgaattac ggtcattaag attataccca
     1741 agtttattca aactcatgcc tataatctct ctagcaccgt caactagtaa aatttggttt
     1801 ttaaatttag gattatacaa tgacttccaa ctatcaaatg attcatttgg atacttttct
     1861 ttattatata aaatacctac agttccaaag aaataaggta aagaatattt attgcctcta
     1921 tcaaatgaca tattcatata atctgaatct
//
LOCUS       DAKTIO010000277         1927 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.286, whole genome shotgun sequence.
ACCESSION   DAKTIO010000277 DAKTIO010000000
VERSION     DAKTIO010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.286"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            109..261
                     /locus_tag="O0293_002765"
     CDS             109..261
                     /locus_tag="O0293_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HCY0281581.1"
                     /translation="MKSKSKQPPNKYVEAFKPYLLTLLYLAIFITLYLIYGSGDTHNN
                     FIYNEF"
     gene            277..1734
                     /gene="dltA"
                     /locus_tag="O0293_002766"
     CDS             277..1734
                     /gene="dltA"
                     /locus_tag="O0293_002766"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HCY0281582.1"
                     /translation="MTDIINKLQAFADANPQSIAVRHTTDELTYQQLMDESSKLAHRL
                     QGSKKPMILFGHMSPYMIVGMIGAIKAGCGYVPVDTSIPEDRIKMIINKVQPEFVFNT
                     TDESFESLEGEVFTIEDIKTSQDPVIFDSQIKDNDTVYTIFTSGSTGEPKGVQIEYAS
                     LVQFTEWMLELNKSGNEQQWLNQAPFSFDLSVMAIYPCLASGGTLNLVDKNMINKPKL
                     LNEMLTATPINIWVSTPSFMEMCLLLPTLNEEQYGSLNEFFFCGEILPHRAAKALVNR
                     FPSATIYNTYGPTEATVAVTSIQITQEILDQYPTLPVGVERPGARLSTTDEGELVIEG
                     QSVSLGYLKNDQKTAEVFNFDDGIRTYHTGDKAKFENGQWFIQGRIDFQIKLNGYRME
                     LEEIETQLRQSEFVKEAIVVPVYKNDKVIHLIGAIVPTTEVTDNAEMTKNIKNDLKSR
                     LPEYMIPRKFEWMEQLPLTSNGKIDRKKIAEVING"
     gene            1731..>1927
                     /locus_tag="O0293_002767"
     CDS             1731..>1927
                     /locus_tag="O0293_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HCY0281583.1"
                     /translation="MIPYGDFTFFLIALIALLPVIILGFLGKRSYIYNGVVTAFMIVL
                     IFSSDKHNLFDQKYLSVQLIS"
BASE COUNT          696 a          283 c          340 g          608 t
ORIGIN      
        1 cattatgaat atcgatataa tgaattgtaa aaaacaaatt ttaaaacgta ttttatattg
       61 aacggtcttt ataaaggcgt ttttactaag aactttaaga ggtgcaatat gaaatctaaa
      121 agtaaacagc cacctaataa atatgttgaa gcattcaaac catatttatt aacactattg
      181 tatttggcaa tatttattac tttatattta atttatggca gtggcgacac acacaataac
      241 ttcatttata atgagttcta atgagggaga cttaatatga cagatattat taacaagctg
      301 caagcgtttg cggatgcaaa tccacaaagc attgctgtta gacacacaac tgatgaatta
      361 acttatcaac agttaatgga tgagtctagt aaattagcac atcgattaca aggtagtaag
      421 aaaccgatga ttttattcgg tcacatgtca ccatatatga ttgttgggat gattggtgcc
      481 attaaagcag gatgtggata tgtacctgta gacacttcaa ttcctgaaga ccgtattaaa
      541 atgattatta acaaggttca accagagttt gtatttaata cgactgatga atcatttgaa
      601 agtttagaag gcgaagtatt tacaatagaa gatattaaaa catctcaaga cccagtaatt
      661 tttgatagtc agattaaaga taacgacaca gtatacacaa tctttacatc tggttctact
      721 ggggaaccta aaggtgttca aattgaatat gcaagtttag ttcaatttac tgagtggatg
      781 ttagaactta ataaatcagg aaatgaacaa caatggctta accaagcgcc attttcattt
      841 gatttatctg taatggctat ttatccatgt ttagcatcag gcggtacatt aaatcttgta
      901 gataaaaaca tgattaataa acctaaatta ttaaatgaaa tgctaacagc aacaccgatt
      961 aacatttggg tatcaacacc atcatttatg gaaatgtgtt tattattacc aacgcttaat
     1021 gaagaacaat atggtagtct taacgaattc ttcttctgtg gtgaaattct acctcacaga
     1081 gcagcaaaag cgttagtaaa ccgtttccca agtgcgacga tttacaacac atatggtcca
     1141 actgaagcta cggtagcagt tacaagtatt caaattacac aagaaatctt agatcaatat
     1201 ccgacattac ctgttggcgt tgaaagacca ggcgcaagat tatctactac agatgaaggt
     1261 gaacttgtta tcgaaggtca aagtgtaagt ttaggatact taaaaaatga ccaaaaaaca
     1321 gctgaagtat ttaatttcga tgacggtatt cgtacatatc acactggtga taaagcgaag
     1381 tttgaaaatg gtcaatggtt cattcaaggt cgtattgatt tccaaatcaa attgaatggc
     1441 tacagaatgg aattagaaga aattgaaaca caattacgcc agtctgagtt cgtaaaagaa
     1501 gcgattgttg tacctgtata taaaaatgat aaagttattc atttaattgg tgcaattgtg
     1561 ccaacgactg aagttacgga caatgcagaa atgactaaaa atattaaaaa tgacttgaaa
     1621 tcacgcttac cagagtatat gattcctaga aagtttgaat ggatggaaca attgccattg
     1681 acttcaaatg gtaaaattga cagaaagaaa attgcagagg taattaacgg atgattccat
     1741 atggtgattt tacattcttc ttaattgctt taattgcatt attaccagtc attatacttg
     1801 gatttttagg taagcgaagt tacatttata atggcgtagt tacagcattt atgattgtgt
     1861 taatcttttc ttctgataaa cataatctgt ttgaccaaaa gtatttaagt gttcaattaa
     1921 ttagttt
//
LOCUS       DAKTIO010000278         1921 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.287, whole genome shotgun sequence.
ACCESSION   DAKTIO010000278 DAKTIO010000000
VERSION     DAKTIO010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.287"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            114..1025
                     /locus_tag="O0293_002768"
     CDS             114..1025
                     /locus_tag="O0293_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-hydroxamate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCY0281584.1"
                     /translation="MKRLIGILLCNLFILTACSASVDKTSNSTKTTDYKIENGETLKV
                     PEKPKRVAVLTGFYVGDFIKLGIKPIAVSDITKDSSILKPYLKGVDYIGENDVERVAK
                     AKPDLIVVDAMDKNIKKYQKIAPTIPYTYNKYNHKEILKEIGKLTNNEDKAKKWIEEW
                     DDKTRKDKKEIQSKIGQATASVFEPDEKQIYIYNSTWGRGLDIVHDAFGMPMTKQYKD
                     KLQEDKKGYASISKENISKYAGDYIFLSKPSYGKFDFEKTHTWQNIEAVKKGHVISYK
                     AEDYWFTDPITLEHLRSKLKKEILNKK"
     gene            1087..1866
                     /locus_tag="O0293_002769"
     CDS             1087..1866
                     /locus_tag="O0293_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0281585.1"
                     /translation="MKQLVGIPESMLIPLIARAKEYENEKPIIKDALSKKIFDGLDDM
                     YKNVTCDDMSQIGISIRTVIIDCVTKRLIKDNKDLIVVNIGCGLDTRFQRFNKEKISW
                     IDLDVPESIEIRKTFFKESNSYKMISKSMLDYSWIDDVKNYKFFNSKSNILFIIEGVL
                     MYFDESVMTQLLDTIIKKMGDHNLTFAIEFCSKTIANNTKRHQSVSKLSSQPVFKYGY
                     NDLKKLNEILPNTIRVIHEYNYFDYYKNRWGLFGYCRFIPY"
BASE COUNT          813 a          196 c          318 g          594 t
ORIGIN      
        1 cataaatgaa aatagaaatg ttactgatgt gtattactaa caggcgttga ctaattcttt
       61 taatatttta taattgaaaa tgaatatcaa ttgaaaaaca aaaggatgat attatgaaaa
      121 gattaattgg aattttatta tgtaatttat ttattttgac tgcgtgttca gcatctgtcg
      181 ataaaacaag taactcgaca aagactacag actataaaat tgaaaatggt gaaacactga
      241 aagtaccaga gaaacctaaa agagttgctg tattaactgg attttatgtt ggtgatttta
      301 taaagttagg aatcaaacca attgctgttt cagatataac taaagattct tcaattttaa
      361 aaccttattt aaaaggggtt gattatattg gagaaaatga tgttgaaaga gttgctaaag
      421 caaaaccaga tttaattgtt gtagatgcta tggataaaaa tataaaaaaa taccaaaaaa
      481 tagcgccaac aatcccatat acatacaata aatacaatca taaagaaata ttaaaagaaa
      541 taggcaagtt gactaataat gaagataaag ctaaaaaatg gattgaagag tgggacgata
      601 aaactagaaa agataaaaaa gaaattcaaa gtaaaattgg tcaagcaaca gcatctgtgt
      661 ttgaaccaga tgaaaagcaa atatacatat ataactctac atggggtcgt ggtttggata
      721 ttgttcatga tgcattcggt atgccaatga caaagcaata taaagataaa ttacaagaag
      781 ataaaaaagg ctatgcttcc atttcaaaag aaaatattag taaatatgct ggtgattata
      841 tatttttaag taaaccttcg tacggaaaat ttgattttga aaaaacacat acatggcaga
      901 atattgaagc tgtaaaaaaa ggacatgtaa tttcatataa agcagaagat tattggttca
      961 cagatcctat tacattagaa catttgagaa gtaaattaaa aaaagaaatt ttaaataaaa
     1021 aataatagaa ataagttgta aaaattttct tatgcattgg tactaatgtt tttaaggagt
     1081 gattaaatga agcaactggt tggaattccc gaatcaatgt taattccttt gatagctcga
     1141 gcaaaagagt acgaaaacga aaaaccaata ataaaagacg cactatcaaa aaaaatattt
     1201 gatggtttag atgatatgta caaaaatgtt acatgtgatg acatgtctca aattggaatt
     1261 agtatacgta ctgtgataat agattgtgtt actaagaggc ttatcaagga taataaagat
     1321 ttaatcgtgg tcaatatagg ttgtggctta gatacaaggt ttcaaagatt taataaagaa
     1381 aaaatatcat ggatagattt agatgtacca gaatcaatag aaatacgaaa aacatttttt
     1441 aaagaaagta atagttataa gatgatatct aaatctatgc tagattacag ttggattgat
     1501 gatgtcaaaa attataaatt ttttaatagt aagtcaaata tattgtttat tattgaaggt
     1561 gtattgatgt attttgatga gagtgtaatg actcaattat tggacactat tatcaaaaag
     1621 atgggagatc ataatttgac atttgcgatt gaattttgct caaaaacaat tgcgaataat
     1681 acaaagagac atcaatcggt atcaaagtta tcctcacaac ctgtttttaa atatggttac
     1741 aatgatttaa aaaaattgaa tgaaatttta ccaaatacaa taagagtaat acatgaatat
     1801 aattattttg actattataa gaatcgatgg ggcttatttg gatattgtag atttatacct
     1861 tattaaaaaa aaaggttaaa caataaaata gtacttatga aatataaagt tccaaagaga
     1921 c
//
LOCUS       DAKTIO010000279         1906 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.288, whole genome shotgun sequence.
ACCESSION   DAKTIO010000279 DAKTIO010000000
VERSION     DAKTIO010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.288"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(158..730)
                     /gene="raiA"
                     /locus_tag="O0293_002770"
     CDS             complement(158..730)
                     /gene="raiA"
                     /locus_tag="O0293_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCY0281586.1"
                     /translation="MIRFEIHGDNLTITDAIRNYIEEKIGKLERYFNDVPNAVAHVKV
                     KTYSNSATKIEVTIPLKNVTLRAEERNDDLYAGIDLINNKLERQVRKYKTRINRKSRD
                     RGDQEVFVAELQEMQETQVDNDAYDDNEIEIIRSKEFSLKPMDSEEAVLQMNLLGHDF
                     FVFTDRETDGTSIVYRRKDGKYGLIQTSEQ"
     gene            complement(791..1465)
                     /locus_tag="O0293_002771"
     CDS             complement(791..1465)
                     /locus_tag="O0293_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HCY0281587.1"
                     /translation="MNNCLSCGAKLYENITIYNLFKKPNRLCDRCKENWDNIKLDIKA
                     RRCSRCLKHLNQDEAYCLDCKFLSAHFNLMEQLYCQFQYDGLMKEMIHQYKFLKDYYL
                     CELLAHLIEIPQTSYDYIVPIPSSPAHDLSRTFNPVEAVLKAKGIRFDKILKMSNRPK
                     QSHLTKKERLADENPFIIDTELDLNGKEILLVDDIYTTGLTIHRAGCKLYAKNIRKFK
                     VFAFAR"
     gene            complement(1458..>1906)
                     /locus_tag="O0293_002772"
     CDS             complement(1458..>1906)
                     /locus_tag="O0293_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA/RNA helicase"
                     /protein_id="HCY0281588.1"
                     /translation="QKMLYRILQDQINNQRYTLVFFNNIETMIKTFSVYKQKITKLTY
                     VHSEDVFRFEKVEQLRNGHFDVIFTTTILERGFTMANLDVVVIDAHQYTQEALIQIAG
                     RVGRKLECPTGKVLFFHEGVSMNMIQAKKEIQRMNKLALKRGWIDE"
BASE COUNT          597 a          348 c          239 g          722 t
ORIGIN      
        1 ttattaactt aacattcaaa tatatacatc cattaacatt agcatagtca ctatgtttca
       61 ttcaacaaat tacattatcg aactatgaaa tagtcataat ttgcttttgg agtataaaaa
      121 agcacttgtg caaaaacaca agtgctttaa acttaattta ttgttcacta gtttgaatca
      181 agccatattt accgtcttta cggcggtaaa cgatacttgt tccatcagtt tctctgtctg
      241 tgaatacaaa gaagtcatga cctaatagat tcatttgtaa taccgcttct tctgaatcca
      301 ttggttttaa gctgaattct tttgaacgaa taatttctat ctcgttatca tcgtaagcgt
      361 cattatcaac ttgtgtttct tgcatttctt gtaattcggc aacaaacact tcttgatctc
      421 ctcgatcacg gctcttacga ttaatacgtg ttttatattt tcgaacttgt ctttcaagtt
      481 tattattaat taaatcaata cctgcgtata aatcatcgtt tcgctcttca gctcttaacg
      541 taacattttt caatggaatt gttacttcaa ttttagtagc tgaatttgaa taagttttaa
      601 ctttaacatg cgccactgca tttggtacgt cattaaaata acgttccaac ttaccaattt
      661 tttcctcaat atagttgcga atagcatctg tgatagtgag gttatctcca tgaatttcaa
      721 atctaatcat agtaatctct ccttaaacct ctttattggt aactctttat tatattttaa
      781 catttttacg ctatcgtgca aacgcaaaca ctttgaattt tctgatattt ttagcatata
      841 atttacaccc tgcacgatga attgttaatc cagttgtata aatatcgtca acgagtaata
      901 tttccttacc atttaaatct aattccgtat caataataaa tggattttca tctgccagac
      961 gctctttctt agttaaatga gactgttttg gtctatttga catctttaaa atcttatcaa
     1021 agcgaatccc tttagctttt agtactgctt ctaccgggtt aaatgttcta gataaatcat
     1081 gtgccggcga agaaggaatt ggcacaatat agtcataaga tgtttgtggt atttcaatca
     1141 aatgtgccaa taattcacat aaataatagt ctttcaaaaa tttatactga tgtatcatct
     1201 ctttcattaa accgtcatat tgaaattgac aatataattg ttccattaaa ttaaagtgtg
     1261 ccgatagaaa cttgcagtct aaacaatacg cttcatcttg atttaagtgt tttaagcacc
     1321 ttgaacatcg ccttgcttta atatcaagtt taatattgtc ccaattctct ttgcatctgt
     1381 cacataatct attaggtttc ttgaacaaat tataaatggt tatattttca tataacttag
     1441 caccacaact caaacaatta ttcatcaatc caacctcttt ttaatgctaa tttgttcatc
     1501 ctttgaatct cttttttagc ttgaatcata ttcatactta ccccttcatg aaaaaacaat
     1561 acttttccag taggacattc taattttcgt ccaacacgtc cagcaatttg tattaaagcc
     1621 tcttgagtat attgatgtgc atcgataaca acaacatcca aatttgccat tgtaaatcca
     1681 cgttctaata ttgtcgtagt aaaaatgaca tcgaaatgtc cattccttaa ttgttcaact
     1741 ttttcaaagc gaaaaacatc ctcgctatgg acgtatgtta atttagtaat tttctgctta
     1801 taaaccgaaa atgttttaat cattgtttct atattgttaa aaaacaccag tgtataacgt
     1861 tgattattaa tttgatcttg taaaattcgg tataacattt tctgaa
//
LOCUS       DAKTIO010000280         1900 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.289, whole genome shotgun sequence.
ACCESSION   DAKTIO010000280 DAKTIO010000000
VERSION     DAKTIO010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.289"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..600)
                     /locus_tag="O0293_002773"
     CDS             complement(<1..600)
                     /locus_tag="O0293_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCY0281589.1"
                     /translation="MSQNTNHSYYHQNQHAQSISKVWLYFMYYWIIFGIGCYLGQFLP
                     LSWRQPLSFGLLIIILATLVFERARRFGLIISHIYAVVIGLLSYATFTTYLQNLGPDI
                     FYKNIALAIFAFIAFGIIGYFFVGDASSIGKYLFVTLITLIIASLIGIFLQNPIFYTI
                     ITVVSLLLFLLYTLYDFNRLKRGDYSPREMGFNLFINLLN"
     gene            961..1758
                     /locus_tag="O0293_002774"
     CDS             961..1758
                     /locus_tag="O0293_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCY0281590.1"
                     /translation="MKKLAFAITATSGAAAFLTHHDAQASTQHTVQSGESLWSIAQKY
                     NTSVESIKQNNQLDNNLVFPGQVISVGGSDAQNTSNTSPQAGSASSHTVQAGESLNII
                     ASRYGVSVDQLMAANNLRGYLIMPNQTLQIPNGGSGGTTPTATTGSNGNASSFNHQNL
                     YTAGQCTWYVFDRRAQAGSPISTYWSDAKYWAGNAANDGYQVNNTPSVGSIMQSTPGP
                     YGHVAYVERVNGDGSILISEMNYTYGPYNMNYRTIPASEVSSYAFIH"
BASE COUNT          708 a          322 c          273 g          597 t
ORIGIN      
        1 attcaacaaa ttaataaata gattaaatcc catttctctt ggtgaatagt cacctctttt
       61 taaacgatta aaatcataca aagtataaag tagaaataac aacagactaa cgacggtaat
      121 aatagtgtaa aaaataggat tttgaagaaa aataccaatt agactcgcaa taattaatgt
      181 tattaatgta acgaataaat atttgcctat actcgatgca tctccaacga agaaataacc
      241 aataatacca aatgctataa atgcaaaaat tgctaatgcg atatttttat agaaaatatc
      301 tggtcctaaa ttttgtaaat acgtggtaaa cgttgcgtat gacaataagc caatcactac
      361 agcgtaaata tgtgaaataa ttaaaccgaa ccgtctcgct ctttcaaaaa caagtgttgc
      421 taaaataata atcagtaatc caaatgacaa gggttgtcgc caacttaatg gtaaaaactg
      481 acctagatag catcctatgc caaatataat ccaataatac ataaaataaa gccacacttt
      541 acttattgat tgagcatgct ggttttgatg ataatatgaa tgatttgtat tttgcgacaa
      601 aataattcct ccttctaccg tcgttttatg cgtcaaattt tacagtaaat tttgcttcat
      661 caaaagaaat aacctttaac aagtatagta attttacatt ttacaatgtt acaaaatata
      721 attttttata attagttaaa atcactaaaa cgcttttata cactatcaaa tcagcattta
      781 taaaaatatg aaccgatatc ctaaaatgtt aataatatta caagataata acaaaccaca
      841 caaagctact tatttttgat aatatggaaa tcgtaatata aaacgaaaac ttaatttact
      901 atataaattg tcttaataat ttttaaaagt agtaaaacat aattttaagg aggagtccct
      961 ttgaaaaaat tagcatttgc aataacagca acatctggtg cagctgcatt tttaacgcat
     1021 catgatgcac aagcttctac acaacataca gtacaatctg gtgaatcatt atggagtatt
     1081 gctcaaaaat acaacacttc agtagagagt attaaacaaa ataaccaatt agataacaac
     1141 ttggtattcc ctggtcaagt tatctcagta ggtggaagtg atgcacaaaa tacgtcaaac
     1201 acttctccac aagctggttc agcatcatct catactgtac aagctggtga atcattaaat
     1261 atcattgcta gcagatatgg tgtttcagtt gatcaattaa tggcagccaa taacttacgt
     1321 ggttatttaa ttatgcctaa ccaaacatta caaattccta atggtggatc aggtggtaca
     1381 acaccaacag ctacaacagg tagcaatggc aatgcatcat cttttaatca ccaaaattta
     1441 tacactgctg gtcaatgtac atggtacgta tttgaccgtc gtgctcaagc tggtagtcca
     1501 attagcacat attggtcaga cgctaagtat tgggctggta acgcagctaa tgatggttac
     1561 caagtaaaca acacaccatc agttggttca attatgcaaa gcacacctgg tccatatggt
     1621 catgttgctt atgttgaacg tgtcaatggt gatggtagta tcttgatttc tgaaatgaat
     1681 tacacatatg gtccatacaa tatgaactac cgtacaattc cagcttcaga agtttctagc
     1741 tatgcattca tccattaatt aaataaattg tactgatata tactagcaat tcacatcatg
     1801 tgagattgct agttttttat ttttgaaaaa aattttcatt ttggtacaaa aaattatctc
     1861 acccttccct atcatacata tttatatttt gtatgaatgg
//
LOCUS       DAKTIO010000281         1857 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.290, whole genome shotgun sequence.
ACCESSION   DAKTIO010000281 DAKTIO010000000
VERSION     DAKTIO010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.290"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            184..1674
                     /locus_tag="O0293_002775"
     CDS             184..1674
                     /locus_tag="O0293_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HCY0281591.1"
                     /translation="MHRESKYIEYKKSRKGLSNDIWSTYSAFANTEGGTIYLGIEEKK
                     IEDKKVFVSVGVEDPEKMIEDFWNALYGRSKVSQNILSNKDVKIVNIENKACIEIHVP
                     EAPYSKKPIYVDNKKDLVYKRVDDADRIATEEEYKFMIVNSQDDIDTELLDNYDMSDL
                     NHESIENYRKLLLKNTNDERYANMSQLDLMIDLGAYRKDRSSKDKQYKMTTACLLFFG
                     KYNAISDRFPGFQLDYFKKTNYLDTDWKDRISSGDLGNEDLNVYSFFEKVLIKLTDNI
                     EESFSLNDGLTRQNYARDLKVAIREALVNTLMHAYYDTKQSIKIVNCEDFIEFYNPGN
                     MRINKEDFIHGGHSKDRNSILSTLFRRVGYSEKAGSGGPRIFDVVNRHKLKTPEIELT
                     DMDTNVVLWKQDLMKEFEKYPELDKKVIKYIIDYGSISKGEALKMENMTEYQFRNILK
                     KLKDDNLIKKEGEGPATKYVLIESKEADILRTKKVIKSLESFFRNK"
BASE COUNT          750 a          183 c          369 g          555 t
ORIGIN      
        1 cgtgaaataa tcagaggaat cgtttgaaat catcgtagta atcgcgcgaa aatcgcgtga
       61 aataatcaga ggaatcgttt gaaatcatcg tagtaatcgc gcgaaaatcg cgtgaaatac
      121 tatggtagac ggtttgagta aattaatgga gtattttaat atttatggtt tggaggaatt
      181 gcgttgcata gagaaagtaa atatatagag tataagaaat cacgaaaagg attatctaat
      241 gatatttggt ctacgtatag tgcttttgca aatactgaag gtggtactat atatttagga
      301 attgaagaaa aaaagatcga ggacaaaaaa gtctttgttt cagttggtgt tgaagatcca
      361 gagaaaatga ttgaagattt ttggaatgca ctatatggaa gaagtaaagt tagtcaaaat
      421 attttatcaa ataaagatgt taaaattgtt aatattgaaa ataaagcgtg cattgaaatt
      481 catgtaccag aagcgcctta ttcgaagaaa ccgatatatg tagataataa aaaagattta
      541 gtatataaaa gagttgatga tgctgataga attgcgactg aagaagagta taaattcatg
      601 attgtaaatt ctcaagacga tatagataca gaattattag ataactatga catgtctgat
      661 ttaaatcacg aatctatcga aaattatagg aaacttctat taaaaaatac taatgatgag
      721 agatatgcga atatgagcca actggattta atgatagatt taggagcata tagaaaagat
      781 agaagttcga aagacaaaca gtataaaatg actacagcat gtttattatt ctttggtaag
      841 tataatgcga ttagtgatag attcccagga tttcaattag attattttaa gaaaacaaat
      901 tacctagata ctgattggaa agatagaata tcaagtggag atttaggtaa tgaagattta
      961 aacgtgtata gtttttttga aaaagtattg ataaaattaa ctgataacat tgaggaatca
     1021 tttagcctaa atgatggttt gactagacaa aattatgcaa gagatttaaa agtagcaatt
     1081 cgcgaagcac tggttaatac attaatgcat gcgtattatg atactaagca aagtattaaa
     1141 atagttaatt gtgaagattt tatagagttt tataatccgg gtaatatgag aataaataaa
     1201 gaagatttta ttcatggagg gcattcaaag gacagaaata gtatattatc gacgcttttc
     1261 agaagagtag gatattcaga aaaagctgga tctggaggac caaggatatt cgatgtagtt
     1321 aatagacata agcttaaaac gcctgaaata gaattaacgg acatggacac taatgtagta
     1381 ctttggaaac aagatttaat gaaggagttt gaaaaatatc ctgagttaga caaaaaagta
     1441 ataaagtata ttattgacta tggatcaata agtaagggtg aagccttaaa aatggaaaat
     1501 atgacagaat atcagtttag aaatatttta aaaaaactaa aagatgataa cttgataaaa
     1561 aaagaaggtg aaggtccggc tactaaatat gtgttaatag aatcaaaaga agctgatata
     1621 ttgcgaacta aaaaagtaat taaaagttta gagtctttct ttaggaataa ataaaaaaac
     1681 aagagatagg tgcgaagtgt ttggattata caaatgcttc gcatcttata tttattaata
     1741 aatatcatag aaaaccgtat cattaaccga tacgcagaga tgcggttttt tagacacttc
     1801 ataaagggat tttgaacgta tcagaacata tgaggtttat aggaattgct gttatgt
//
LOCUS       DAKTIO010000282         1856 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.291, whole genome shotgun sequence.
ACCESSION   DAKTIO010000282 DAKTIO010000000
VERSION     DAKTIO010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.291"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(192..1127)
                     /gene="trxB"
                     /locus_tag="O0293_002776"
     CDS             complement(192..1127)
                     /gene="trxB"
                     /locus_tag="O0293_002776"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCY0281592.1"
                     /translation="MTEIDFDIAIIGAGPAGMTAAVYASRANLKTVMIERGIPGGQMA
                     NTEEVENFPGFEMITGPDLSTKMFEHAKKFGAVYQYGDIKSVEDKGEYKVINFGNKEL
                     TAKAVIIATGAEYKKIGVPGEQELGGRGVSYCAVCDGAFFKNKRLFVIGGGDSAVEEG
                     TFLTKFADKVTIVHRRDELRAQRILQDRAFKNDKIDFIWSHTLKSINEKDGKVGSVTL
                     TSTKDGSEETHEADGVFIYIGMKPLTAPFKDLGITNDVGYIVTKDDMTTSVPGIFAAG
                     DVRDKGLRQIVTATGDGSIAAQSAAEYIEHLNDQA"
     gene            complement(1194..>1856)
                     /locus_tag="O0293_002777"
     CDS             complement(1194..>1856)
                     /locus_tag="O0293_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281593.1"
                     /translation="YRASQQLLYPLYKKGKFLSIQMYNALAYNYYYLGEEDESHYYWD
                     KLKQISKVEIGHAPWVIENSKEVFDQHILPLLQSDDSHYRLYGIFLLDQLNGKEIVMT
                     ESIWQVLENLNNYEKLYLTYLVQGLTLNKLDFIHRGLLTLYHNELFVSENDVMVAWIN
                     QGELIIAEKVDLTDVEPYIGAFIYLYFKNQPRNVTKKQITTWLGITQYKLNKMIEFLL
                     SI"
BASE COUNT          595 a          367 c          238 g          656 t
ORIGIN      
        1 aagttataag tttaatataa catataaaaa aactagaact cattgaaacg cacaaatgat
       61 acgtactaag ttctagtttt ctatataatt tgctttaatt taaaagtaag acattatttt
      121 aacgctgtga tgacactatt aaaataaata tccaaataat ttacagctca acatcttaat
      181 tcgacttcga attaagcttg atcgtttaaa tgttcaatat attccgctgc actttgcgct
      241 gcaatactac catcgccagt agcagtgaca atttggcgta aacctttgtc gcgaacatct
      301 cctgctgcaa aaatacctgg tactgatgtt gtcatatcat cttttgttac aatataacca
      361 acatcatttg taatacctaa gtctttaaat ggcgctgtta atggtttcat accaatatag
      421 atgaatacac catcagcctc gtgtgtttct tctgaaccat cttttgtaga cgttaatgtc
      481 acagaaccca ctttgccgtc tttttcatta attgatttca aagtatgact ccaaataaag
      541 tcgattttat catttttgaa tgctctatct tgtaaaatac gctgtgcacg taactcatca
      601 cgacggtgaa cgattgttac tttgtcagca aatttagtta agaatgttcc ctcttctact
      661 gctgaatcac caccaccgat aacgaatagg cgtttatttt taaagaatgc accatcacat
      721 actgcacaat aacttacacc gcgtccacca agttcttgtt cacccggaac accaattttc
      781 ttgtattctg cacctgtagc aataataacc gctttcgctg ttaattcttt attaccaaag
      841 ttaatcactt tatattcgcc tttatcttct acagatttaa tatctccata ttgataaact
      901 gcaccaaact ttttagcgtg ttcaaacatt tttgtagata aatctggacc tgtaatcatt
      961 tcgaaaccag ggaagttctc tacttcttct gtattagcca tttgaccgcc tggaatacct
     1021 ctttcaatca taactgtttt taaattagca cgtgatgcgt atactgcagc agtcatacca
     1081 gctggacctg caccgataat tgctatatca aaatctattt cagtcatttt attaacgcct
     1141 cctcattatt aatcattatg cgcattatat aataaatcta acttttcata aatctatatg
     1201 ctcaagagaa attcaatcat tttgttcagt ttatattgtg ttatgcctaa ccatgttgta
     1261 atttgcttct ttgtaacgtt tcgaggttga tttttaaaat acaaataaat aaacgcaccg
     1321 atatatggct caacatcagt taaatctact ttttcagcaa ttatgagttc accttgatta
     1381 atccatgcaa ccattacatc attttcactt acaaataatt cattatggta aagcgttaat
     1441 aagccgcgat gaatgaagtc taatttattg agcgttaaac cttgaactaa atacgttaaa
     1501 tacaatttct cataattatt tagattttcc aaaacctgcc aaatactttc cgtcatcaca
     1561 atttctttac catttaattg atccaataaa aaaataccat ataaacgata atgactgtca
     1621 tcactttgaa gtaatggcaa aatatgttgg tcaaaaactt ctttgctatt ttcaattacc
     1681 caaggcgcat gtccaatttc cactttagaa atttgcttca atttatccca gtagtaatga
     1741 ctttcgtctt cttcacctaa ataataataa ttatatgcta aagcattgta catttgaatt
     1801 gataaaaatt tccctttttt atataaaggg tacaacaatt gttgtgatgc acgata
//
LOCUS       DAKTIO010000283         1846 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.292, whole genome shotgun sequence.
ACCESSION   DAKTIO010000283 DAKTIO010000000
VERSION     DAKTIO010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.292"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..299)
                     /locus_tag="O0293_002778"
     CDS             complement(<1..299)
                     /locus_tag="O0293_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCY0281594.1"
                     /translation="MKTNILNLDNELENTEYVEGKPYVQYGIKYERNQALRNEAIKIH
                     GTTCKVCGFDFKAKYGDLGEGFIEIHHLKPMFSIKREIKVNPQKDLVPLCSNCHK"
     gene            complement(296..433)
                     /locus_tag="O0293_002779"
     CDS             complement(296..433)
                     /locus_tag="O0293_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001791883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281595.1"
                     /translation="MELKLLNKLDDDQLDLEFLQDNGLKGNVQGPMTLKEPLKSYIQK
                     S"
     gene            489..1807
                     /gene="cstB"
                     /locus_tag="O0293_002780"
                     /pseudo
     CDS             489..1807
                     /gene="cstB"
                     /locus_tag="O0293_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002488497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
BASE COUNT          634 a          302 c          279 g          631 t
ORIGIN      
        1 attttatggc aattagaaca tagtgggact aaatcttttt gtggatttac ttttatttct
       61 ctttttattg aaaacattgg ttttaaatga tgaatttcaa taaaaccctc acctaaatcg
      121 ccatacttag ctttaaaatc aaatccacat actttacatg tagttccatg aattttaata
      181 gcttcatttc ttaatgcctg atttctttca tatttaatac cgtactgtac atatggttta
      241 ccttcgacat attcagtgtt ttctaactca ttatctaaat ttaaaatatt cgttttcaag
      301 acttttgtat ataacttttt aaaggttcct ttaaagtcat aggaccttga acattacctt
      361 ttaacccatt atcttgtaaa aattctaaat ctaattggtc atcatcaagt ttgtttaata
      421 actttaattc catgtaatta ccatagttaa tataattatc acccttgata acaaattgac
      481 tatcatcaat ttatgataaa catttatctc aagcatctta tttaatcggt tgtcaaaaaa
      541 ctggagaagc catgatcatt gatcctattt gtgacttatc ttcatatatt cgagttgccg
      601 atgaagagag tttaaccatt actcatgcag ctgaaacaca tatacatgca aattttgttt
      661 caggaattag agatgttgct ataaagttaa atgctagtat ttatgtatcg ggcgaaagtg
      721 atgacatgtt aggttataaa aatatgccta accacactca ttttgttcaa cataatgatg
      781 atatttatgt aggaaatata aaattaaaag tgcttcatac acctggtcac acgccagaaa
      841 gtataagttt tttacttact gacgaaggtg ctggagcaca agttccaatg ggactattca
      901 gtggtgattt tatttttgta ggagatatcg gtagacctga tttactagaa aaagctgtta
      961 aagtagaagg attatctgaa ataggcgcaa aacaaatgtt taaatctatt gaaagtatta
     1021 aagacttgcc agactacatt caaatttggc ctggtcatgg cgctggtagt ccttgtggta
     1081 aatctttagg tgctattcca acatctacgc ttggctatga aaaacaaacg aactgggctt
     1141 tttctgaaaa taatgaagct acctttatcg ataaactcat ttctgaccaa cctgcaccac
     1201 cacatcattt tgcacaaatg aaaaaaatta atcaattcgg tatgaattta tatcaacctt
     1261 atacggttta tccagctaca agtacaaaca gattaacttt tgatctccgc agtaaggagg
     1321 cttatcatgg tggacatatt gaaggtacaa tcaatattcc atatgataaa aatttcatca
     1381 atcaaattgg ctggtatcta aactataatc aagaaattaa cttgattgga gactatcacc
     1441 ttgtttcaaa agcaacacat aacatacttt acaacttatt ggatatgata atgtgtctgg
     1501 atatcagtta ccacaagctc aaattcaaac acaatctgtt catagtaaag acataactgg
     1561 taatgaagca catgtcttag atgtacgtaa tgataatgaa tggaataatg gccacttatc
     1621 ccaagcggtt catgtgccac acggcaaact tttagaaaca gatttacctt tcaatagaaa
     1681 cgatgttatt tatgtagact gtcagtctgg cattagaagt tctatagcta ttggtatttt
     1741 agaacacaaa ggttatcaca acattattaa tgtaaacgaa ggttacaaag atatatacct
     1801 ttcttaataa ttatatttat actaatgaag aaggactata agtagt
//
LOCUS       DAKTIO010000284         1833 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.293, whole genome shotgun sequence.
ACCESSION   DAKTIO010000284 DAKTIO010000000
VERSION     DAKTIO010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.293"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            154..276
                     /locus_tag="O0293_002781"
     CDS             154..276
                     /locus_tag="O0293_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281596.1"
                     /translation="MKVIFYCPKRADDILKASMEYQSTDYKRRYISWKTMIAMK"
     gene            252..350
                     /locus_tag="O0293_002782"
                     /pseudo
     CDS             252..350
                     /locus_tag="O0293_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429703.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="head morphogenesis protein"
     gene            659..796
                     /locus_tag="O0293_002783"
     CDS             659..796
                     /locus_tag="O0293_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281597.1"
                     /translation="MILIMLDSQLDGNVTAKELSESFKELVEEFESIEKAKKNSNSKD
                     K"
     gene            858..1118
                     /locus_tag="O0293_002784"
                     /pseudo
     CDS             858..1118
                     /locus_tag="O0293_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282612.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            1167..1515
                     /locus_tag="O0293_002785"
                     /pseudo
     CDS             1167..1515
                     /locus_tag="O0293_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456552.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1526..1720
                     /locus_tag="O0293_002786"
     CDS             1526..1720
                     /locus_tag="O0293_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281598.1"
                     /translation="MRIEDAYRKDVIITLLNNEEYEGFVTDYENEFESETGNLVVDIQ
                     TDFAVYSFDETEIKSIRLLK"
BASE COUNT          767 a          196 c          314 g          556 t
ORIGIN      
        1 aaaaatctag tttagaaaaa aatacaagag ataattattt tattcatgcc atagacagaa
       61 tatacataaa tactagtaaa gggctatttc ctgaatctga actcgtagca tggggataaa
      121 gaaagtttac tttaaaaatt ttaaccttca aatgtgaagg ttatttttta ttgcccaaaa
      181 cgtgctgatg acattttaaa agcaagtatg gaatatcagt cgacagacta taaacggagg
      241 tatatctcat ggaaaacaat gatagcaatg aagtagctta tttgttaaaa gatggtaaat
      301 ttacaaaagt atatgtcaat caagatagtg tatcttttgt gccagggtaa caagcaaccg
      361 aattattatt taacagtaaa ccgaattcaa ttgttatgtt acataaccat cctggacagt
      421 caggattttc agaatatgat ttatttacat tttttaagca tccatcaata aaatcaatga
      481 caatcgttac taataaggga caagtaaaat ttattaccaa gtcaaatagg tttcatggta
      541 aaatagtgag taaattttgt gctaaatatt ttacgcatat taatatcatt aatgatagcc
      601 atattgaaaa attattgaaa aaactttata gtataaatat gattaaatat aaagtgagat
      661 gattttaatt atgttagatt cacaattaga tggaaatgta actgcaaaag aattgagtga
      721 atcttttaaa gaattagttg aagaatttga aagtatagaa aaagcgaaaa agaattcaaa
      781 ttcaaaagat aaatagcatc ctttttagac agataaagag aaagtggtgc tatttttata
      841 cgctttttta actatttact gaaaatgcag aagcacaaaa gttaacatat ttaaaagaac
      901 ttggtgaaga tggcgaatat aaatatgttg ccaaaataga tagtaaaaca tctaaattat
      961 gtcattcact taacggaaaa atatttaaag ttaaagatat gataccaggt gtgaatgcgc
     1021 cacctatgca tccttggtgt agaagtacca cagtgccaca tgttggcaat tggcgggaca
     1081 agttctttaa agagcgtgaa ggtaaatatc aagtagaagt aaaagaagca taattacagg
     1141 aaaaagctaa aaaccagatg aaagaaatga ttaaaaatgg taaaataaaa atagaaataa
     1201 attgcggaaa aataaaatag atatatgtta ggtcatcacc tatataatga aaataaaaaa
     1261 agagccattt taaataataa gaaattgcct agctatacaa tactttctat agatttattg
     1321 aatgaattgt taagagaaaa aatgtcaaca ggcaatctaa tattaagtga tgagcgattt
     1381 gatttgaaag agattattaa ttttaatcaa attattggaa aaatacatat cgaaaatgtg
     1441 tatattgaaa ccagaaaggg aaaagtgcat tattcgaaga caggtgctca tatagtacct
     1501 tatattgata agtaggtgaa aagtattgag aattgaagat gcatatcgta aagatgttat
     1561 tatcacgctt ttgaataatg aagaatacga agggtttgta actgactatg aaaatgaatt
     1621 cgagagcgaa acaggaaatc ttgttgtaga tatacagact gattttgctg tttattcgtt
     1681 tgatgaaact gagataaaaa gtattagatt attaaaataa ttacaaaata gcatcctttc
     1741 tacaaaaagc taaaaaagaa atgcttgaaa tgataagtaa tggtaaaatg acgatgaaat
     1801 tgaataatgt taagcaaaag cgtcatattt tat
//
LOCUS       DAKTIO010000285         1831 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.294, whole genome shotgun sequence.
ACCESSION   DAKTIO010000285 DAKTIO010000000
VERSION     DAKTIO010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.294"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            158..883
                     /gene="sasD"
                     /locus_tag="O0293_002787"
     CDS             158..883
                     /gene="sasD"
                     /locus_tag="O0293_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070002951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasD"
                     /protein_id="HCY0281599.1"
                     /translation="MKKLATVGSLIVTSTLVFSSMPFQNAHADTTSMNVSNKQSQNVQ
                     NHRPYGGVVPQGMTQAQYTELEKALPQLSAGSNMQDYNMKLYDATQNIADKYNVIITT
                     NVGVFKPHAVRDMNGHALPLTKDGNFYQTNVDANGVNHGGSEMVQNKTGHMSQQGHMN
                     QNTHMNQQPHMQQGHMQSSNHQMMSPKANMHSSNHQMNQSNKKVLPAAGESMTSSILT
                     ASIAALLLVSGLFLAFRRRSTNK"
     gene            complement(1075..1830)
                     /locus_tag="O0293_002788"
     CDS             complement(1075..1830)
                     /locus_tag="O0293_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCY0281600.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLARV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
BASE COUNT          636 a          301 c          318 g          576 t
ORIGIN      
        1 tattgacatt aaataaattg acaaaataag taattattgt gaatctaaag tgaaattttt
       61 ataaaaaaat gtaatgattc aaaaattttg ttgcatttct tttgtaatcg tatgataatg
      121 taaatgtaat caaattgtaa tataagggga caagacaatg aaaaaattag caacagtagg
      181 ttctttaatt gtaacaagca ctttagtatt ctcaagtatg ccttttcaaa atgcgcatgc
      241 cgacacaact tcaatgaatg tgtcgaataa acaaagccaa aatgtacaaa atcatcgtcc
      301 ttatggcgga gtagtaccac aaggaatgac gcaagcacaa tatactgaat tagagaaagc
      361 tttaccccaa ttaagcgctg gcagtaatat gcaagactat aatatgaaat tgtatgatgc
      421 gacgcaaaat attgctgata aatacaatgt gataattaca actaatgtag gggtatttaa
      481 accacatgct gttagagata tgaatggcca tgcgttacct ttaacaaaag atggcaattt
      541 ttatcaaacg aatgtagatg caaatggtgt taatcatggt ggtagtgaaa tggtgcaaaa
      601 taaaacaggt catatgagtc aacaaggcca tatgaatcag aacacacaca tgaaccaaca
      661 gccacacatg caacaaggtc atatgcaatc atcaaaccat caaatgatga gtccaaaagc
      721 aaatatgcat tcatcaaatc atcaaatgaa ccaaagtaac aaaaaagttt taccagctgc
      781 tggtgaaagt atgacatcaa gtattcttac tgcaagtatt gccgcactac tattagtatc
      841 tgggttattc ttagcattta gacgacgttc aacaaataaa taaacataat acgattaata
      901 atagaaaaat cgtgtgatta tctgagcgag cctaggacat aaatcaatgt cctaggctcg
      961 ctaatgttat attggcagta gttgactgaa tgaaattgcg cttgtaacaa gcttttccat
     1021 ttcttaccaa ctccctaaat agtcatcaaa aaatttctta tattttaata atttttaata
     1081 atccgattgt cttatacgtg tcagtgttaa ttcagatatt tcctgtggaa tataccactt
     1141 attaatcata attggataag gtgtttgtgc gtacagtgtt tcaataatca gccaacaatg
     1201 tgtatcacca tcaaacacgt gactatgatt tttgaagtgg ggcgctttgg taatagacat
     1261 ttttaaatct gattgatatg cattgctata aatcgtttgc tcaacgaatg tcttcatgtc
     1321 gtcttcgttt tgtgtattca ctttaaatgt gtcaatgaca tttaacggta taaaggtaaa
     1381 gcaaaatgca tcagcttgct tagaatgatt gtcctttttt tgataatagc gttccattgc
     1441 aatgacggca gaaggatggt ttgcaaacaa atgatttgta tattcacttt ctaaatcaac
     1501 acgataatta attgatgaca tagatacgcg agctagcaat atttgatcaa gtggatgctt
     1561 aaattgatcc atacttgaag cgtgttgggc atttgtttgt ggaataacaa agtgtccctt
     1621 ccctcttgta ctctctacga tgccatcttc ggctaacaat tttatagctt ggcgcaaagt
     1681 catacgactg acatcaaagc gcgcacaaag ttccttttca gtaggtaatg catggccact
     1741 cggatatttt cctatttgaa tttctttata taacgtatta taaatcgtta aaaattttgg
     1801 ttgtgtttgc gtcacgtaga caacctccat a
//
LOCUS       DAKTIO010000286         1811 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.295, whole genome shotgun sequence.
ACCESSION   DAKTIO010000286 DAKTIO010000000
VERSION     DAKTIO010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.295"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            163..1368
                     /locus_tag="O0293_002789"
     CDS             163..1368
                     /locus_tag="O0293_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCY0281601.1"
                     /translation="MWIEKFKNKNNETKYRYYEKYKDPYTDKWKRVSVVLNKNTKQSQ
                     KEAMFRLEEKIKEKLNNKSSSELKTLTFHALLDEWLEYHIKTSGSKLTTLNNIKIRIR
                     NIKRYSSENLLLNKLDTKYMQIFINKLSDIYSQNQVTRQLGDMKGAIKYAVKFYNYPN
                     EYLLTNVKIPKRRKTIEDIEKDESKMYNYLEMNQVLQIRDHILNDNKLHKRNRILIAS
                     ILEVQALTGMRIGELQALQEKDIDLLNKTINITGTIHRIKYEEGFGYKDTTKTISSKR
                     SISINSRTVEIFKKIILENKMLKRWNSSYVDRGFIFTTKKGNPLCNNQIAGVLKKTTK
                     ALNMNKKVTTHTFRHTHITLLVEMNVSLKAIMKRVGHVDEKTTIRIYTHVTEKMDREL
                     TQKLENIPS"
     gene            complement(1411..>1811)
                     /locus_tag="O0293_002790"
     CDS             complement(1411..>1811)
                     /locus_tag="O0293_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281602.1"
                     /translation="KIADYVQGIYDEYASKIVDVIKYLINTIPKIRKNLRHAIEMLNV
                     KKKEFLSLIPNVTCNYIKTKLEELDNTLGKWEPFLNDLKAVSPILDNHLDDIVKNMKP
                     LIVQMIFEPSHYDDMFISRKALTPVFSSVL"
BASE COUNT          734 a          277 c          257 g          543 t
ORIGIN      
        1 ctcctgtaag aagaagcgcc cctagaaatt gagtcatctt atcacctact ttttatttta
       61 ttataacaca tttagtacct agtactaaat tacgggtagc ccgactaccc ttattatttt
      121 ttaatatttt atagaacata cgttcttgca ggaggtataa acatgtggat tgaaaaattt
      181 aaaaacaaaa ataacgaaac taaatacaga tactacgaga agtataaaga tccatacaca
      241 gataaatgga agcgcgtaag tgttgtgttg aacaagaata caaaacaatc tcaaaaagaa
      301 gcaatgtttc gtttagaaga aaaaataaaa gaaaaactga acaacaagtc gtcaagcgaa
      361 ttaaaaactt tgacttttca cgcgctatta gatgaatggc ttgaatatca tataaaaaca
      421 tcaggttcaa agttgactac tcttaataat ataaaaataa gaattagaaa cattaaacga
      481 tacagctctg agaacttgct tttaaacaaa ctagatacaa aatatatgca gatatttatt
      541 aataaattat cagatatcta ttctcaaaat caagtaaccc gtcaactcgg agatatgaaa
      601 ggagctatta aatatgcagt taaattttac aattatccaa atgaatattt gttaactaat
      661 gtcaaaattc ctaaaagaag aaaaacaata gaggatatcg aaaaagatga atctaaaatg
      721 tacaactatt tagaaatgaa ccaagtccta cagatacgtg atcatatact aaatgataat
      781 aagttacaca agcgaaatcg cattttaatt gccagcatct tagaagtaca ggctttaact
      841 ggtatgcgca taggagaact acaagcactg caggaaaaag atatagattt attaaacaaa
      901 actatcaata taacaggtac aattcaccgc attaaatacg aggaaggatt cggatacaaa
      961 gacactacaa agactataag ttcaaaaaga agtatcagca tcaattctag aaccgtagaa
     1021 atttttaaaa agataatact ggaaaacaaa atgttgaaaa gatggaattc gagctatgtt
     1081 gacagagggt tcatattcac aacaaaaaaa gggaatcctt tatgtaataa tcaaatcgcc
     1141 ggtgtgctta agaaaactac aaaagcttta aatatgaata agaaagttac cacgcacaca
     1201 tttagacata cacacataac tttattagta gaaatgaatg tttctttaaa agcaattatg
     1261 aaaagggtag gacatgtaga tgaaaaaaca accattcgca tatatactca tgtaactgaa
     1321 aaaatggata gagaactaac tcaaaaactc gaaaacattc caagttagct taaatccgcc
     1381 ctttttttgc ccttatattt tttacaagct ttataaaacg cttgagaaca ctggcgttaa
     1441 agcttttctt gaaataaaca tatcatcata atgtgatggt tcaaatatca tttgtacaat
     1501 caaaggcttc atgttcttaa caatatcatc taaatggtta tctaaaattg gtgacactgc
     1561 ttttaaatca ttaagaaaag gctcccattt gcctaaagta ttatctaatt cttctaattt
     1621 agttttaata taattacaag ttacattagg aatcagggac aaaaattctt tcttttttac
     1681 atttaacatt tcaattgcat gtcttaaatt cttacgtatt ttgggaattg tattaatcaa
     1741 atattttatt acatcgacaa ttttcgatgc atattcatca tatatacctt gaacatagtc
     1801 tgctattttt t
//
LOCUS       DAKTIO010000287         1791 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.297, whole genome shotgun sequence.
ACCESSION   DAKTIO010000287 DAKTIO010000000
VERSION     DAKTIO010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.297"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            308..862
                     /locus_tag="O0293_002791"
     CDS             308..862
                     /locus_tag="O0293_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HCY0281603.1"
                     /translation="MNKKLLTKTLIASALVLTTVGSGFHSSSNYNGINNVAKAEQTTD
                     NELWKNVRDALKEANIIDKTDNENVKVTYKLANGGENSIEGTANLNELSNSNNKPINS
                     DSVKSIDLSRKNPNGDRFDANDAWKKLTDKLKEKQIVKDGDTVTIHSKDEKDPKISAK
                     VGEVYNDNKRLMLFKKDIDKITIN"
     gene            complement(1097..>1791)
                     /locus_tag="O0293_002792"
     CDS             complement(1097..>1791)
                     /locus_tag="O0293_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HCY0281604.1"
                     /translation="HIKSRISYYFSEKYREPHSYLYFKNYSSDTSKTDSIVKMVATFS
                     SVMSNRKNKPLKHYINTHNGVPLWILVNYLTLGNVSKMYSNLDDDLRLEVAKDYKRKL
                     ERDYKTRVQITPSDVDSILQQAHMFRNVCAHEERLYDYKIDRAKSRANIFANYNKIYD
                     KEYVPTMNGSYVFDLLISLCLFLNKHDYIKLMKNMDKLISNYSHSFYTITIDDLYTKM
                     NFPDQTKILDML"
BASE COUNT          687 a          247 c          245 g          612 t
ORIGIN      
        1 atgtaattat aacataaaaa aatagacaag taccgaagta cctgcctaaa taacaacaag
       61 attaacatgt gaataatgga aataaaaagt cagcccgaag gctaacttac gaatagatga
      121 aaatttgaac acattgctgt gtctaaaatg attatagcat aaataacgaa tatttccagc
      181 tcaaaattaa tatattgtaa taataatatt ttatatcttt gttaataatt atttaattga
      241 tttacataaa taataattgt aaaattactt tgtaatcgat tgcaaataaa ttataggaga
      301 aaataaaatg aataaaaaac tattaacaaa aacattgata gcaagtgctt tagttttaac
      361 aacagtaggt tcaggttttc attcttcttc aaattataat ggtattaata acgttgcaaa
      421 agctgagcaa acgacagata acgaattgtg gaaaaatgta agagacgctt taaaagaagc
      481 gaatattatc gataaaacag ataatgaaaa tgtcaaagtg acttataaat tagcaaatgg
      541 cggtgaaaac agtattgaag gtaccgccaa tttaaatgaa cttagcaatt ctaataacaa
      601 accgatcaat agcgatagtg taaaaagcat tgatttgtca aggaaaaatc ccaatggaga
      661 tagatttgac gcaaatgatg catggaaaaa attaactgat aaattaaaag aaaagcagat
      721 tgtgaaagac ggtgatacag taactattca tagtaaagac gaaaaagacc ctaaaatctc
      781 agctaaagtc ggtgaagtct ataatgataa caaacgattg atgttgttta aaaaggatat
      841 agataaaatc actataaact agttatagat aaagacaggt tactttttta atgtaacttg
      901 tcttttttta agaggcagta tctcaaatat taatttataa tttctaaatc tatatctata
      961 ctttatctat ttctaaatct atatctatac tttatctatt tctatataca cactcatccc
     1021 tcactgcaac acagggcgtt tctcagcgtc ttaaaataaa aaaacgccac tcgtaagtga
     1081 cattaaaaaa tatcttttat aacatatcca gtatttttgt ttgatctgga aaattcattt
     1141 ttgtatatag gtcatctata gtaattgtat agaaagaatg tgaataatta cttattagtt
     1201 tatccatatt tttcatcaat tttatgtaat catgtttatt caaaaataga catagtgaaa
     1261 tcaacaaatc gaatacataa ctaccattca ttgtaggaac gtattcttta tcgtatattt
     1321 tgttataatt ggcgaatata ttagctctac ttttagccct gtctatttta taatcataca
     1381 atctttcttc atgcgcacac acgttacgaa acatatgtgc ttgttgtagt atactgtcta
     1441 catctgatgg agttatttga acacgtgttt tataatctct ttccaatttc cttttatagt
     1501 ctttagcaac ttccaatcga agatcatcat ccaaattgga atacattttt gaaacattac
     1561 ctaaagttaa ataattcacc aatatccata gtggcactcc attatgagta ttaatataat
     1621 gttttaatgg tttatttttt ctattactca taaccgagct aaatgtagca accattttca
     1681 cgatgctatc tgtcttactt gtgtcagatg aataattttt aaagtataaa tatgaatgtg
     1741 gttctctata tttttcgcta aaataatatg aaattcttga tttaatatga g
//
LOCUS       DAKTIO010000288         1782 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.298, whole genome shotgun sequence.
ACCESSION   DAKTIO010000288 DAKTIO010000000
VERSION     DAKTIO010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.298"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            142..255
                     /locus_tag="O0293_002793"
                     /pseudo
     CDS             142..255
                     /locus_tag="O0293_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
     gene            612..1112
                     /locus_tag="O0293_002794"
     CDS             612..1112
                     /locus_tag="O0293_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0281605.1"
                     /translation="MTFEEKLSEIYNKIANEISGMIPVEWDQVFTIAYVNDRGGEIVF
                     NYTKPGSDELNYYTYIPREYNVSEKVFYDLWTDLYRLFKKLRNAFKEEDLEPWTSCEF
                     DFTRDGKLNVVFDYVDWMNSEFGPIAKENYYMYKKFGVLPETEYEINKVKEIDQYVKE
                     QDEAEL"
     gene            1124..1624
                     /locus_tag="O0293_002795"
     CDS             1124..1624
                     /locus_tag="O0293_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0281606.1"
                     /translation="MTFEEKLSQMYNKIASEISGMIPVEWEQVFTIAYVTDQAGEVIF
                     NYTKPGSDELNYYSDIPKDCNVSKDIFKNSWFKVYRMFDELRETFKEEELEPWTSCEF
                     DFTRDGKLNVSFDYIDWVNSEFGPMGREHYYMYKKFGIWPEKEYAINWVEKIKDYVKE
                     QDEAEL"
     gene            1635..>1782
                     /locus_tag="O0293_002796"
     CDS             1635..>1782
                     /locus_tag="O0293_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF600 family protein"
                     /protein_id="HCY0281607.1"
                     /translation="MTFEGKLSQMYNEIANEISGMIPVEWEQVFTIAYVTDQAGEVIF
                     NYTKP"
BASE COUNT          689 a          168 c          345 g          580 t
ORIGIN      
        1 aattaaacac tgaagatgat gactaaataa ttttataaaa tactgttgga gtatacttaa
       61 ataagaaaat caacttcaac gtatatcacc atattatttt atactcaatg atgtgaaaga
      121 atttgagtgg attgatatta atgggatttg attattagaa ttagaaaagg aacgtttgct
      181 tttgttgtaa taaatctatt atttttcaat tgttggttat ttattctgaa tataatttta
      241 tcaagattat tataattgta ttacatatag gtgctggtaa attttattta cgaaatggca
      301 aaaaagtata tactattaat atcttttttg ctataattat aaatagttaa ataaatagaa
      361 agacaaaata atgagtagga gttttagggc tattatttac aaataatata atactgcaaa
      421 gactcgaaat tatttagaat tccagttatc gtaacaatag ttgcagttat tttagttcca
      481 ttagtgtatt taggtttaaa atgagtaata ttaatttgaa gaagcattaa gcgacgtaga
      541 ggtgttgttt agtgcttttt atttaacgga aatgaaacca atatctattt tgaaaatata
      601 ggggcgataa catgactttc gaagaaaaac taagtgaaat atacaacaag attgcgaatg
      661 agattagtgg gatgatacca gtagaatggg accaagtatt tacgatagct tatgtaaatg
      721 atagaggtgg agagatcgtt tttaattata ctaaaccagg tagcgatgaa ttgaattatt
      781 acacatatat ccctagagag tataatgtct ctgaaaaagt attttatgat ttgtggacgg
      841 atttatatag attgtttaag aagttaagaa acgcatttaa agaagaagat ttagaaccat
      901 ggacatcatg tgaatttgac tttacaagag atggtaaatt gaatgttgta tttgattatg
      961 ttgattggat gaattcagaa tttggtccaa tagcaaaaga aaattattat atgtacaaaa
     1021 agtttggtgt tttaccagaa acggaatatg aaattaataa agttaaagaa atcgatcaat
     1081 atgttaaaga gcaagatgaa gctgaactat agggggagat aacatgactt tcgaagagaa
     1141 actaagtcaa atgtacaata aaattgcaag tgagatcagt ggaatgatac cagtagaatg
     1201 ggagcaagta tttacaatag cttatgtaac tgatcaagct ggggaagtca tttttaatta
     1261 tactaaacca ggtagtgatg aattaaatta ttattcagac atacctaaag attgcaatgt
     1321 ctcaaaagat atttttaaga attcatggtt taaagtttat cgaatgtttg atgagttaag
     1381 agaaactttt aaagaagaag agcttgaacc atggacatca tgcgaatttg actttacaag
     1441 agatggcaaa ttgaatgtat cttttgatta tattgattgg gtgaattcag aatttggacc
     1501 aatgggaaga gaacattatt atatgtataa aaaatttgga atttggcctg aaaaagaata
     1561 tgccataaat tgggttgaaa aaataaaaga ttatgttaaa gagcaagatg aagctgaact
     1621 ataggggaga taatatgact ttcgaaggaa aactaagtca aatgtacaac gagattgcga
     1681 atgagattag tgggatgata ccggtagaat gggagcaagt atttacaata gcttatgtaa
     1741 ctgatcaagc tggagaagtc atctttaatt atactaaacc ag
//
LOCUS       DAKTIO010000289         1725 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.299, whole genome shotgun sequence.
ACCESSION   DAKTIO010000289 DAKTIO010000000
VERSION     DAKTIO010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.299"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..646
                     /locus_tag="O0293_002797"
     CDS             <1..646
                     /locus_tag="O0293_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HCY0281608.1"
                     /translation="FSFYNAGIEKIYGSPIIERNERMIRLSDAFTNALSNQTFNMFLE
                     DLIELSKYNNEKYQKGKNGLILYNKYSREDFSKIFNWNKNGSSVIMGYMIKSQEMPIF
                     ITYDKHEDISDSTKYEDKFLSQDELKWFTKSNRTLESKEVQKILSHRAKGIKMYIFVQ
                     KKDDDGIYFYYLGTAGYIEGSEKQDKMPNGSNVVTMDLALDKAVRDDIYRYLTN"
     gene            952..1725
                     /locus_tag="O0293_002798"
     CDS             952..1725
                     /locus_tag="O0293_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0281609.1"
                     /translation="MIHSKRLRLWLYLVLLAVFIGACGMKKEESSKDKQIKENFNKTL
                     SLYPTKNLEDFYDKEGFRDQEFEKGDKGTWIIHSKMTIETNGKNMESRGLVLYVDRNT
                     RTTKGEFIMRELWEDKKGYSRSKEKEYPVKMEHNKIIPTKPIADDKLRKEIENFKFFV
                     QYGDFKDINDYKDGDISYNPNVPSYSAKYQLSNDDYNVKQLRKRYDIPTKKAPKLLIK
                     GDGDLKGSSIGHKNLEFTFIENKEENIYFTDSINFKPTE"
BASE COUNT          731 a          188 c          296 g          510 t
ORIGIN      
        1 tttttcattt tacaacgcag gaattgaaaa aatctatgga agtcccatta tagaacgtaa
       61 tgaaagaatg ataagactaa gcgatgcatt tactaatgct ctaagtaatc aaacatttaa
      121 tatgttttta gaagatctta ttgaattatc aaagtataac aatgaaaaat accaaaaagg
      181 taaaaatgga ctgattttat ataataaata ttcaagagaa gattttagta aaatatttaa
      241 ttggaataaa aatggttcgt ctgtaatcat gggatacatg ataaaaagcc aagaaatgcc
      301 aatatttatc acttatgata agcatgagga tataagtgat agcacaaaat atgaagataa
      361 atttttaagt caagatgaat taaaatggtt tactaaatct aatagaacgc tagaatcaaa
      421 agaagttcag aaaattttgt cccatagagc aaaaggaatt aaaatgtata tttttgtaca
      481 gaaaaaagat gatgatggta tatattttta ttatttagga actgctggat atatagaagg
      541 ttcagaaaag caagataaaa tgccgaatgg ttcaaacgta gtaacgatgg atttagcact
      601 ggataaagct gtaagagatg atatatatcg ttatctaaca aattaatgta aaccgaaaaa
      661 tgaatgttag taaagttgta tttaaacatc aattttaata agtaaataca ttgagtttca
      721 gtgttattat tttaaatata agcgcaattt atcttaatta attagttggt taggccaata
      781 aaaattataa agatatttgc gctttttaaa taatttgtga tgttgatatg tgaaaaatac
      841 aactaacaat taaaatccac gaggatttaa tcataaaatt tcctctaata ttattaatat
      901 tggaatttga acttaacttt gcatcaaaaa taaaaatggg gatgtgaaat gatgattcac
      961 tcaaaaaggt tgaggctctg gctgtattta gtattattag ctgtttttat aggggcttgc
     1021 ggaatgaaaa aggaagaaag cagtaaagat aaacaaatta aagaaaactt caacaaaacg
     1081 ttgagcctgt atccaactaa aaatcttgaa gacttttatg ataaggaagg ctttcgagat
     1141 caagagtttg aaaagggcga caaaggcact tggataattc actctaaaat gacaatagaa
     1201 acaaatggaa agaatatgga atccagagga ttggtcctgt atgtagaccg caataccaga
     1261 acgacaaaag gtgaatttat tatgagggaa ttatgggaag ataaaaaagg atactcacgt
     1321 agtaaagaaa aagaatatcc agtaaaaatg gaacataata aaattatccc aaccaaaccg
     1381 atagctgatg ataaattaag aaaagaaatc gaaaacttta aattctttgt acaatacggt
     1441 gactttaaag atattaatga ttataaagat ggtgacattt catataatcc gaatgtgcca
     1501 agttattcag caaagtatca attaagtaat gatgactaca atgtgaagca acttagaaag
     1561 aggtatgata taccaactaa gaaagcacct aaattattaa taaaaggcga tggtgattta
     1621 aaaggatcat ctataggaca caaaaattta gagtttacat ttatagaaaa taaagaagaa
     1681 aacatctatt ttacggatag tattaatttc aaacctactg aatag
//
LOCUS       DAKTIO010000290         1707 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.300, whole genome shotgun sequence.
ACCESSION   DAKTIO010000290 DAKTIO010000000
VERSION     DAKTIO010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.300"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..522)
                     /locus_tag="O0293_002799"
     CDS             complement(<1..522)
                     /locus_tag="O0293_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCY0281610.1"
                     /translation="MYNFDYSKLPIKNIQKIFPIAGGYVNLSFSVDASNKKYFLKLQP
                     NTKSNFFDYELSSLKELTDKNIPVPQIINKGELDNNSFLLLEFIENGHAYPESYRKLG
                     KIVANMHKNINSLNLFGFSHNFNGGTIEFTNKWTSSWGDLFIKSRMDKLCSEIYKKRL
                     FSISDLLLYEDIRK"
     gene            complement(592..>1707)
                     /locus_tag="O0293_002800"
     CDS             complement(592..>1707)
                     /locus_tag="O0293_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HCY0281611.1"
                     /translation="DHYNPNNEITKSRNTLDNWALSRLNTLIKESNIYVNNYDFTSAA
                     RLINEYTNTISNWYIRRSRGRFWEQGISNDKKDAYNTLYEILTTLSRLVAPFVPFISE
                     KIHYNLTGKSVHLQDYPQYKESFINQALEDEMHTVIKIVELSRQARKNADLKIKQPLS
                     KMVIKPNSQLNLSFLPNYYSIIKDELNIKNIELTDNINDYITYELKLNFSSVGPKLGN
                     KTKNIQTLIDSLSEYDKKSLIESNNFKSLSSDAELTKDDFIIKTLPKDSYQLSEDNDC
                     VILLDKNLSPELIREGHARELIRLIQQLRKKKNLPINQRIDIYIGVTGELLESIKTNK
                     NMFKENLLIKNIHLNVIDEYENTIHFNNKEIKISLLY"
BASE COUNT          561 a          235 c          216 g          695 t
ORIGIN      
        1 ttttcgtata tcttcatata ataaaagatc cgaaattgaa aataaacgtt tcttgtatat
       61 ttcagaacat agcttatcca tcctactttt aataaataaa tccccccaag aagaagtcca
      121 tttattagtg aattcaatcg ttcctccatt gaaattatga ctaaagccaa atagatttag
      181 tgaatttata tttttatgca tgtttgcaac tattttacct agttttctat agctttcagg
      241 ataagcatgg ccattttcaa tgaactctaa cagtaaaaaa gaattattgt ctaattctcc
      301 tttattaata atttgaggaa caggaatatt tttatcagtt aattctttta aactggataa
      361 ctcataatca aaaaaatttg atttagtatt aggttgtagc tttaaaaaat attttttgtt
      421 ggaagcatca actgaaaaag atagattcac atatccccct gcaataggaa atattttttg
      481 aatattttta attggtaatt tagaataatc aaaattatac atagtttatc accttttgct
      541 tggctttgat aactcatggc cactctacta aaatagagtg gccatgtttg tttaatataa
      601 taaggaaatt tttatttctt tattattaaa atgaatagta ttttcatatt catctataac
      661 atttaagtgt atatttttaa tcagcaaatt ttctttaaac atatttttat tggtttttat
      721 tgattctaat aattccccag ttacaccgat ataaatatca atacgttgat ttattggtaa
      781 atttttcttt tttcttaatt gttgaattaa tctaatgagc tctctagcat gtccttcgcg
      841 aattaattca ggagataaat ttttatctaa taatataacg cagtcattat cttcactgag
      901 ttgataacta tccttaggta aggttttaat tataaaatca tccttagtta actcagcatc
      961 agaagataaa cttttgaagt tattagactc aattaaactt tttttatcat actctgatag
     1021 ggagtctatc aatgtttgaa tatttttcgt tttgttccct agttttggtc ccacagaaga
     1081 aaaattcaat ttaagctcat aggtaatata gtcattaata ttatcagtta attcaatgtt
     1141 ttttatattt aattcgtctt ttattattga atagtaatta ggtaaaaaac ttaagtttaa
     1201 ttgactatta ggtttaatca ccattttcga taaaggttgc ttaattttta aatctgcatt
     1261 tttgcgagcc tgtctagata attctacaat ttttataacg gtatgcattt catcttccaa
     1321 tgcttgatta ataaaacttt ctttatattg tggataatct tgtaaatgca cactttttcc
     1381 agtcaaatta taatggattt tttcagatat aaatggaaca aatggagcca ctagtcttga
     1441 taaagttgtt aaaatttcat aaagcgtatt gtacgcatct tttttatcgt tagaaattcc
     1501 ttgttcccaa aatcgtcctc tcgatctccg aatataccaa ttacttattg tattggtata
     1561 ttcgttaatt aatctggctg cggaagtgaa atcgtaatta tttacataaa tattactttc
     1621 ttttattaag gtgtttaagc gagaaagagc ccaattatct aatgtatttc tactttttgt
     1681 aatttcatta ttaggattat agtgatc
//
LOCUS       DAKTIO010000291         1637 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.301, whole genome shotgun sequence.
ACCESSION   DAKTIO010000291 DAKTIO010000000
VERSION     DAKTIO010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.301"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            259..453
                     /locus_tag="O0293_002801"
     CDS             259..453
                     /locus_tag="O0293_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCY0281612.1"
                     /translation="MADESKFEQAKGNVKETVGNVTDNKNLENEGKEDKASGKAKEFV
                     ENAKEKATDFIDKVKGNKGE"
     gene            complement(686..1537)
                     /locus_tag="O0293_002802"
     CDS             complement(686..1537)
                     /locus_tag="O0293_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HCY0281613.1"
                     /translation="MQQFKWINILKGFAMGTSDLVPGVSGGTIALLLGIYNQFIASIS
                     GIFSRRFWPSFTFLIPIIIGMLLAMGSLSNLFNYLLSQHHIPTMFFFGGLIIGIVPYL
                     LKISNYKTSFTTKHYMMVIAGIAILIVITLMNNGDKHAGETLTLSTSLIIKYFIAGMC
                     ASSAMLLPGISGSFMLLVFGVYGTVMLAISEVVKLNFAGLPILLAVGFGVLAGFIISS
                     KIIQYFLTHHKLMTFALIIGFVVGSLFAVFPGLPTNIVMWFVSLVVFIIGFIVSLTLG
                     RITAENE"
BASE COUNT          652 a          250 c          245 g          490 t
ORIGIN      
        1 agtaaaatcc tatatgcatg cgtgctactc atttattctt atttttaatt tcacatggct
       61 agtgactaat acatcataaa tcattagtga ttctttatta tttcagtccc actccctcaa
      121 gttaatgatg aatctatttc gctagttata aaatgacgtc aaatcaatat caaaaattat
      181 ttaagtaaaa tgtttagata atttttcagt gggtaagtat tatatataac atttaattat
      241 tccgaggagg catttattat ggcagacgaa agtaaatttg aacaagcaaa aggtaatgtt
      301 aaagaaacag taggtaatgt tactgataat aaaaatttag aaaacgaagg taaagaagat
      361 aaagcttctg gtaaagcgaa agaattcgtt gaaaatgcaa aagaaaaagc aactgatttt
      421 attgataaag taaaaggtaa caaaggcgag taattttact ttttattcct aatacactgc
      481 acatgccact tagtcggtat aaccatcggc taggtggttt ttgtattaaa aaagtggata
      541 ccaaaattta ttaataatta ttttaatgtt agaaaaaaac taaataaaaa ctcgctaatg
      601 atatccaata atatgtatac aaaacgagac atatattgca tatgattaac gagatactga
      661 aaatatttta tcacccctaa aatgattatt cattttcagc ggtaattcga cctaaagtca
      721 aacttacaat aaaaccgatg ataaatacta ctaatgaaac gaaccacatc acgatattag
      781 ttggtaaacc tggaaatact gcaaagaggg agccaacaac aaaaccaatg attaatgcaa
      841 aagtcattag tttatgatgt gttaggaaat actggataat tttgcttgaa ataatgaatc
      901 cagcaagcac gccaaatccg actgcaagta atataggaag acctgcaaag ttaagtttaa
      961 caacttcaga tattgctagc atgaccgtac catagacgcc aaatactaat aacataaatg
     1021 accctgaaat acctgggagt aacatagcac tagatgcaca catacctgca ataaaatatt
     1081 taataataag actagttgat agagtaagtg tttctccagc atgtttatca ccattattca
     1141 ttaatgtaat aacaattaag atagcgatac cagctataac catcatgtaa tgtttagttg
     1201 taaatgacgt tttatagtta gaaattttca ataaatatgg aacgatacca atgattaatc
     1261 caccaaagaa aaacatagtt ggaatatggt gttggcttaa taaataatta aaaagattac
     1321 ttagtgatcc cattgccagt aacattccaa ttataatggg gattaaaaat gtaaaacttg
     1381 gccaaaaacg tcgtgagaat atgccgctaa ttgaagcgat aaattgattg taaataccta
     1441 acaataatgc gatagtccca ccgctaacac caggtaccaa gtcactcgtt cccatagcaa
     1501 aaccttttag aatattaatc catttaaatt gttgcatgaa taactccttt caaacgattg
     1561 gaataaaatc ataaatagca tcataccata ttacaaatgt cctagtgaaa tgataacata
     1621 ttttaaattc ataaaat
//
LOCUS       DAKTIO010000292         1615 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.302, whole genome shotgun sequence.
ACCESSION   DAKTIO010000292 DAKTIO010000000
VERSION     DAKTIO010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.302"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(221..1525)
                     /gene="eno"
                     /locus_tag="O0293_002803"
     CDS             complement(221..1525)
                     /gene="eno"
                     /locus_tag="O0293_002803"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCY0281614.1"
                     /translation="MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTG
                     EHEAVELRDGDKSRYLGKGVTKAVENVNEIIAPEIIEGEFSVLDQVSIDKMMIALDGT
                     PNKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDA
                     PIAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTE
                     DAVETIIQAIEAAGYKPGEEVFLGFDCASSEFYENGVYDYSKFEGEHGAKRTAAEQVD
                     YLEQLVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQLVGDDLFVTNTEILAKGIEN
                     GIGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDELFETAKYDGIKSFYNLDK"
BASE COUNT          490 a          309 c          246 g          570 t
ORIGIN      
        1 ttaagaatac aagttaaata tgtgttatta gttacctcat gattattagt ctgttatgat
       61 taatacttca aaataaaaaa cctaccacag tctcaactct gtggcaggct ataaatttaa
      121 gcgtgaactt atttaagcgt gaactcaaaa tctttattta atttataccg tcataataat
      181 cctcaactaa aattatgtca gcatttgatt ataaagaaaa ttatttatct aagttataga
      241 atgatttgat accgtcatat ttagcagttt caaataattc atcttcgata cgtaataatt
      301 gattgtattt agcaatacgg tcagtacgtg ataatgaacc agttttaatt tgaccagcgt
      361 ttgtagcaac agcaatatca gcaattgttg tatcttctgt ttcacctgaa cggtgagaaa
      421 ctactgctgt gtaaccagct ttttgagcca tttcgattgc atcaaatgtt tcagttaatg
      481 taccgatttg gttaacttta attaagattg agttaccaat tccgttttca ataccttttg
      541 ctaaaatttc agtgtttgtt acgaataaat cgtcacctac taattgtaca cggtcaccga
      601 tacgttctgt aagttgtttc caaccatccc agtcgttttc gtccataccg tcttcaattg
      661 taatgatagg atatttgtct actaattgtt ctaagtagtc aacttgttct gcagctgtac
      721 gttttgcacc gtgttcgcct tcgaacttac tgtagtcata tacaccattt tcatagaatt
      781 ctgatgatgc acagtcaaat cctaagaata cttcttcacc tggtttgtaa ccagctgctt
      841 cgattgcttg gataattgtt tcaacagcat cttcagtacc ttcaaattta ggagcgaaac
      901 caccttcgtc acctactgca gtttctaaac cacgtttgct taaaattgat tttaagttgt
      961 ggaaaatttc agtaccccaa cgtaatgatt ctttgaacgt tgtagcacct acaggtaaaa
     1021 tcatgaattc ttggaatgca attggagcat ctgagtgaga accaccatta acgatgttca
     1081 tcattggtac tggtaactgc ttaccattaa atccacctaa atatttgtaa agtggttgac
     1141 ctaataagtc agctgctgca cgtgctactg cgatagatac acctaaaata gcatttgcac
     1201 ctaatttacc tttgtttgga gtaccgtcta atgcgatcat cattttatca atagatactt
     1261 gatctaatac tgaaaattca ccttcaataa tttctggtgc gatgatttca ttaacgtttt
     1321 caactgcttt agtaacacct ttacctaaat aacgtgattt gtctccatca cgtaattcaa
     1381 cagcttcgtg ttcaccagtt gaagcacctg atggtactaa tgcacgacca aatgcgccac
     1441 tttcagttaa tacttctact tcaacagttg ggttaccacg agagtctaag acttcgcgag
     1501 cgtaaacatc tgtaataatt ggcatgttta taatctcctt taaatagtta ttctataatt
     1561 attatagctt tattctttca tttaacaaaa tgatattact attattttca aaaaa
//
LOCUS       DAKTIO010000293         1598 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.303, whole genome shotgun sequence.
ACCESSION   DAKTIO010000293 DAKTIO010000000
VERSION     DAKTIO010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.303"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(623..1408)
                     /locus_tag="O0293_002804"
     CDS             complement(623..1408)
                     /locus_tag="O0293_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HCY0281615.1"
                     /translation="MKVQIYQLPIVFGDSSKNETQITQWFEKNMNAEVDVVVLPEMWN
                     NGYDLEHLNEKADNNLGQSFSFIKHLAEKYKVDIVAGSVSNIRNNQIFNTAFSVNKSG
                     QLINEYDKVHLVPMLREHEFLTAGEYVAEPFQLSDGTYVTQLICYDLRFPELLRYPAR
                     SGAKIAFYVAQWPMSRLQHWHSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINP
                     NGDLVGELNESADILTVDLNLNEVEQQRENIPVFKSIKLDLYK"
BASE COUNT          560 a          268 c          231 g          539 t
ORIGIN      
        1 cagtgaacaa attcactaaa aaataagatg aataattaat tactttcatt gtaaatttgt
       61 tatcttcgta tagtactaaa agtatgagtt attaagccat cccaacttaa taaccatgta
      121 aaattagcaa gtgagtaaca tttgctagta gagttagttt ccttggactc agtgctatgt
      181 atttttctta attatcatta cagataatta tttctagcat gtaagctatc gtaaacaaca
      241 tcgatttatc attatttgat aaataaaatt tttttcataa ttaataacat ccccaaaaat
      301 agattgaaaa aataactgta aaacattccc ttaataataa gtatggtcgt gagcccctcc
      361 caagctcgcg gccttttttg taatgaagaa gggatgagtt aatcatcatt atgagacccg
      421 ccgttaaaat atatgaataa gtctaatgtt ggaaaaggtc aaaaaattaa tcaatttaat
      481 taagaaaatc attcatttgc aaagggcgaa atgggttctt actgagttat ctattataaa
      541 aaaataaaca tagacttatg aaaaatctct cataaatcta tgtttagtca tgacatgtgt
      601 taaatattat ttcgggcgct tcttatttat acaaatctaa tttaatactt ttaaatacag
      661 gtatattttc gcgttgctgt tctacttcat ttaagtttaa atctacagtc aaaatatctg
      721 cggattcatt taattctcca actaaatctc catttgggtt tataactatc gaatgaccag
      781 catattctgt gttaccatcg aatccagtgc tattagttcc aatgacaaac atattatttt
      841 caattgcacg tgcctttagt aatgaatgcc aatgttgaag acgtgacata ggccattgcg
      901 ccacataaaa tgcaatttta gcaccactac gagcaggata tcttaataat tctggaaaac
      961 gtaaatcata acagataagt tgggtcacat aagtaccgtc agacaattga aagggttcag
     1021 ctacgtattc gccagcggtt aaaaattcat gctctcttaa cataggaact aaatgaactt
     1081 tgtcgtattc attaatcagc tggccacttt tattcacact aaaagctgta ttaaatattt
     1141 gattgtttct aatgttagaa actgacccag ctacgatatc gactttatat ttttcagcta
     1201 aatgtttaat aaatgaaaaa ctttgtccta gattattatc tgctttttca tttaaatgct
     1261 ctaaatcata gccattattc cacatttcag gtaaaacgac tacatctact tcagcattca
     1321 tatttttttc gaaccattgc gttatttgag tttcattttt agaactatct ccaaaaacaa
     1381 tcggtaattg ataaatttgg actttcataa catcacatcc ttgatagatc ttatatataa
     1441 cttactaaaa gttatgttga aacgcaaaaa acgagcacaa gacataaaat caaagtccta
     1501 ggctctacaa agttatattg acagtagttg atggggcccc aacatagaga aattggaaca
     1561 ccaatttcta cagacaatgc aagttggggt gggctcta
//
LOCUS       DAKTIO010000294         1591 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.304, whole genome shotgun sequence.
ACCESSION   DAKTIO010000294 DAKTIO010000000
VERSION     DAKTIO010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.304"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..158)
                     /locus_tag="O0293_002805"
     CDS             complement(<1..158)
                     /locus_tag="O0293_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin A"
                     /protein_id="HCY0281616.1"
                     /translation="MEQIELFSIDKFKCNSEAKYYLNIIEGEWHPQDLNDSPLKFILS
                     TSDDSDYI"
     gene            complement(189..1355)
                     /gene="scpA"
                     /locus_tag="O0293_002806"
     CDS             complement(189..1355)
                     /gene="scpA"
                     /locus_tag="O0293_002806"
                     /EC_number="3.4.22.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain A"
                     /protein_id="HCY0281617.1"
                     /translation="MKRNFPKLIALSLIFSLSVTPIANAESNSNIKAKDKKHVQVNVE
                     DKSVPTDVRNLAQKDYLSYVTSLDKIYNKEKASYTLGEPFKIYKFNKKSDGNYYFPVL
                     NTEGNIDYIVTISPKITKYSSSSSKYTINVSPFLSKVLNQYKDQQITILTNSKGYYVV
                     TQNHKAKLVLKTPRLEDKKLKKTESIPTGNNVTQLKQKASVTMPTSQFKSNNYTYKEQ
                     YINKLENFKIRETQGNNGWCAGYTMSELLNATYNTNKYHAEAVMRFLHPNLQGQRFQF
                     TGLTPREMIYFGQTQGRSPQLLNRMTTYNEVDNLTKNNKGIAVLGSRVESRNGMHAGH
                     AMAVVGNAKLDNGQEVIIIWNPWDNGFMTQDAKNNVIPVSNGDHYRWYSSIYGY"
BASE COUNT          515 a          237 c          217 g          622 t
ORIGIN      
        1 caaatgtaat cagaatcgtc tgaggtactg agaataaatt ttaaaggact atcatttaaa
       61 tcttgaggat gccattctcc ctcaataata ttaagataat acttagcctc tgaattacat
      121 ttgaatttat caatactaaa taattcaatt tgttccataa tattatttac ctttctaaaa
      181 tacaaatttt aataaccata aatagatgaa taccatcgat aatggtcgcc attggatact
      241 ggaataacat tgtttttagc atcttgagtc ataaaaccat tatcccatgg attccatata
      301 attataacct cttgtccatt atctaattta gcgttcccaa caactgccat ggcatgccct
      361 gcgtgcatac catttcttga ttctactcta ctacctaaaa cagcaattcc tttattattt
      421 ttagtaagat tgtcaacttc attatatgta gtcattctat taagaagttg tggacttctt
      481 ccctgagttt gtccaaaata aatcatctct cttggcgtta aaccagtaaa ttggaatcgt
      541 tgtccttgta agtttgggtg taaaaatctc atcacagctt ctgcatgata tttgttagta
      601 ttataagtcg catttagtaa ttcagacatc gtatagcctg cacaccaacc attgttacct
      661 tgagtttctc ttatcttgaa attctcaagt ttatttatat attgctcttt gtaagtataa
      721 ttattacttt taaattgact agttggcata gtgacagaag ctttttgctt tagttgcgtt
      781 acattattgc cagtaggtat actctcagtc ttttttaact ttttatcttc tagacgtggt
      841 gtttttagta ctagtttagc tttatgattt tgagtaacca catagtaacc ttttgagttt
      901 gtcaaaatag taatttgttg atctttatat tgatttaaga ctttagataa aaatggagaa
      961 acattgattg tgtatttaga agatgaacta gaatacttag tgatttttgg tgaaattgta
     1021 actatataat cgatattacc ttcagtgttc aaaactggaa aataataatt accatcactc
     1081 tttttgttga atttataaat tttaaaaggt tcaccaagtg tatacgaggc tttctcttta
     1141 ttatagattt tatcaagtga agtaacataa gaaagatagt ctttttgcgc taaattacgt
     1201 acgtcagtag gaacactctt gtcctcgaca ttaacttgca catgcttctt atcttttgct
     1261 ttgatatttg aattgctctc agcgtttgca ataggggtaa cacttaaaga aaatattaaa
     1321 cttaaagcta ttaattttgg aaagtttctt ttcatataaa aactccttta ttttattata
     1381 aaataattat attaaattta gttaagagtt tcaatataaa attttaatct atgtttaaaa
     1441 aataattaat ttttacacat tatcagttta tgtaataatt tagtagctta tttactaaat
     1501 tgcaaacatc tcattcaata attaatatga tcgaaagttt catgaaaata tattagtcga
     1561 ttttgaaaga agcgtagtca atttatgtct t
//
LOCUS       DAKTIO010000295         1571 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.305, whole genome shotgun sequence.
ACCESSION   DAKTIO010000295 DAKTIO010000000
VERSION     DAKTIO010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.305"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..519
                     /locus_tag="O0293_002807"
     CDS             <1..519
                     /locus_tag="O0293_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HCY0281618.1"
                     /translation="SEGFSIIGLILDVVIMGTLIFYYFIGARFVDHDLSNFLMFMDTG
                     SYIFMYFAIKCLWVKPKVVNYLIAKELGESKEVIEEQELDLQTSKIRGIYFFIISVVM
                     LIITKLRMQPELQADAISMNPVFIFVGVIIILIWLVLDIYRKKKYGIFLFKTIVPLVV
                     TTWIIIATIILS"
     gene            complement(662..1555)
                     /locus_tag="O0293_002808"
     CDS             complement(662..1555)
                     /locus_tag="O0293_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0281619.1"
                     /translation="MKKTILLTMTTLTLFSMSPNSAQAYTNDSKTLEEAKKAHPNAQF
                     KVNKDTGAYTYTYDKNNTPNNNHQNQSRTNDNHQHANQRDLNNNQYHSSLSGQYTHIN
                     DAIDSHTPPQTSPSNPLTPAIPNVEDNDDELNNAFSKDNKGLITGIDLDELYDELQIA
                     EFNDKAKTADGKPLALGNGKIIDQPLITSKNNLYTAGQCTWYVFDKRAKDGHTISTFW
                     GDAKNWAGQASSNGFKVDRHPTRGSILQTVNGPFGHVAYVEKVNIDGSILISEMNWIG
                     EYIVSSRTISASEVSSYNYIH"
BASE COUNT          439 a          227 c          309 g          596 t
ORIGIN      
        1 agtgaaggtt tttcaataat aggtttaata ttagatgttg ttataatggg gacattgatt
       61 ttttactatt ttataggcgc tcgctttgtt gatcatgatt taagcaactt tttaatgttt
      121 atggatactg gatcatatat ttttatgtac tttgcaatta agtgtttatg ggtgaaacct
      181 aaggtagtaa attatttgat tgcaaaagaa ttgggtgaat ctaaagaggt cattgaagag
      241 caggaattag atttacagac atcaaagata agaggtatct actttttcat tatttcagta
      301 gtgatgttaa ttatcactaa attaagaatg caacctgagt tacaggcaga tgctatatcg
      361 atgaatcctg tatttatttt tgttggtgtt ataatcattt taatttggct agtactggat
      421 atatatcgca agaaaaaata cggtatattc ttattcaaga cgatagtgcc actagttgtt
      481 acgacttgga ttattatagc tacaattata ctttcgtaaa cgattttgat taatggggaa
      541 tgtagagaac atttctttgt aattttataa tcgaataaca tacaaaataa agcgaccaat
      601 tttgcagtgt tacgaattgt aaactgcgaa ctggtcgctt tttattgatg tcatgattaa
      661 tttaatggat gtaattatat gatgaaactt ctgaagcaga gatggttctt gatgaaacga
      721 tatattcacc aatccagttc atttctgaaa ttagaatact tccatcaata ttaacttttt
      781 caacgtaggc tacatgacca aatggaccat ttactgtttg taaaattgat cctcgtgttg
      841 ggtgtctatc tactttgaag ccattgcttg aagcttggcc tgcccagttt ttagcatctc
      901 cccaaaatgt actaatcgtg tgtccatctt tggcacgttt atcaaagaca taccatgtac
      961 attgtccagc agtatataag ttgttcttac ttgtgataag aggctgatca atgattttac
     1021 cgttacctaa tgctaaaggt ttaccgtcag cggtctttgc tttgtcatta aattcggcga
     1081 tttgtaattc gtcatacaat tcgtctaaat cgatgcctgt aataagccct ttgttatctt
     1141 ttgaaaaagc gttatttaat tcatcgtcat tgtcttcgac attcggtatt gctggtgtca
     1201 aaggattgct tggtgacgtt tgaggcggtg tgtgtgaatc aattgcgtca ttaatgtgcg
     1261 tatactgacc acttaatgaa gaatggtact gattgttgtt aagatcacgt tgatttgcgt
     1321 gttgatgatt gtcgtttgta cgtgactggt tttgatgatt gttgtttggc gtgttgtttt
     1381 tgtcatatgt ataagtatac gcgccggtgt ctttattcac tttgaactgt gcgtttgggt
     1441 gtgctttctt tgcttcttct aatgttttgc tatcattcgt atatgcttga gccgagttag
     1501 gcgacatact aaataaagta agagttgtca tcgtcagtaa aattgttttc ttcataataa
     1561 ccatttaatc c
//
LOCUS       DAKTIO010000296         1541 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.306, whole genome shotgun sequence.
ACCESSION   DAKTIO010000296 DAKTIO010000000
VERSION     DAKTIO010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.306"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..685
                     /locus_tag="O0293_002809"
     CDS             <1..685
                     /locus_tag="O0293_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HCY0281620.1"
                     /translation="IIMDGNGRWAKKRKMPRIKGHYEGMQTIKKITRVASDIGVKYLT
                     LYAFSTENWSRPESEVNYIMNLPVNFLKTFLPELIEKNVKVETIGFTDKLPKSTIEAI
                     NNAKEKTANNTGLKLIFAINYGGRAELVHSIKNMFDELHQQGLNSDIIDETYINNHLM
                     TKDYPDPELLIRTSGEQRISNFLIWQVSYSEFIFNQKLWPDFDEDELIKCIKIYQSRQ
                     RRFGGLSEE"
     gene            692..1474
                     /locus_tag="O0293_002810"
     CDS             692..1474
                     /locus_tag="O0293_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCY0281621.1"
                     /translation="MKVRTLTAIIALIVFLPILLKGGLVLMIFANILALIALKELLNM
                     NMIKFVSVPGLISAVGLIIIMLPQHAGPWVQVIQLKSLIAMSFIVLSYTVLSKNRFSF
                     MDAAFCLMSVAYVGIGFMFFYETRSEGLHYILYAFLIVWLTDTGAYLFGKMMGKHKLW
                     PVISPNKTIEGFIGGLFCSLIVPLAMLYFVDFNMNVWILLGVTLILSLFGQLGDLVES
                     GFKRHFGVKDSGRILPGHGGILDRFDSFMFVLPLLNILLIQS"
BASE COUNT          522 a          192 c          296 g          531 t
ORIGIN      
        1 tattattatg gatggtaatg ggcgatgggc taagaagcga aaaatgccta gaattaaagg
       61 tcattacgaa ggtatgcaaa caataaaaaa aattactagg gtagctagtg atattggtgt
      121 taagtactta actttatacg ccttttccac tgaaaattgg tcaagacctg aaagtgaagt
      181 aaattatatt atgaatttgc ctgtcaattt cttaaagaca ttcttaccgg aactaattga
      241 aaaaaatgtc aaagttgaaa caattggatt tactgataag ttgccaaaat caacgataga
      301 agcaattaat aatgctaaag aaaagacagc taataatacc ggcttaaaat taatatttgc
      361 aattaattat ggtggcagag cagaacttgt tcatagtatt aaaaatatgt ttgacgagct
      421 tcatcaacaa ggtttaaata gtgatatcat agatgaaaca tatataaaca atcatttaat
      481 gacaaaagac tatcctgatc cagagttgtt aattcgtact tcaggagaac aaagaataag
      541 taatttcttg atttggcaag tttcgtatag tgaatttatc tttaatcaaa aattatggcc
      601 tgactttgac gaagatgaat taattaaatg tataaaaatt tatcagtcac gtcaaagacg
      661 ctttggcgga ttgagtgagg agtagtatag tatgaaagtt agaacgctga cagctattat
      721 tgccttaatc gtattcttgc ctatcttgtt aaaaggcggc cttgtgttaa tgatatttgc
      781 taatatatta gcattgattg cattaaaaga attgttgaat atgaatatga ttaaatttgt
      841 ttcagttcct ggtttaatta gtgcagttgg tcttatcatc attatgttgc cacaacatgc
      901 agggccatgg gtacaagtaa ttcaattaaa aagtttaatt gcaatgagct ttattgtatt
      961 aagttatact gtcttatcta aaaacagatt tagttttatg gatgctgcat tttgcttaat
     1021 gtctgtggct tatgtaggca ttggttttat gttcttttat gaaacgagat cagaaggatt
     1081 acattacata ttatatgcct ttttaattgt ttggcttaca gatacagggg cttacttgtt
     1141 tggtaaaatg atgggtaaac ataagctttg gccagtaata agtccgaata aaacaatcga
     1201 aggattcata ggtggcttgt tctgtagttt gatagtacca cttgcaatgt tatattttgt
     1261 agatttcaat atgaatgtat ggatattact tggagtgaca ttgattttaa gtttatttgg
     1321 tcaattaggt gatttagtgg aatcaggatt taagcgtcat ttcggcgtta aagactcagg
     1381 tcgaatacta cctggacacg gtggtatttt agaccgattt gacagcttta tgtttgtgtt
     1441 accattatta aatattttat taatacaatc ttaatgctga gaacaaatca ataaacgtaa
     1501 agaggagttg ctgagataat ttaatgaatc tcagaactcc t
//
LOCUS       DAKTIO010000297         1529 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.307, whole genome shotgun sequence.
ACCESSION   DAKTIO010000297 DAKTIO010000000
VERSION     DAKTIO010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.307"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            42..221
                     /locus_tag="O0293_002811"
     CDS             42..221
                     /locus_tag="O0293_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281622.1"
                     /translation="MKFKTTKECKSNNIFKRSQEINNRESEKGCLWGISMLILLFLLI
                     LFGITACSSSIHFIN"
     gene            532..792
                     /locus_tag="O0293_002812"
     CDS             532..792
                     /locus_tag="O0293_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281623.1"
                     /translation="MCIVIYLIYSLIVYFICIINFFIMAAPFLMTNADYVWTPMTTVT
                     LFILSLIIFLIFIKTKDVVHLTIFILNLLFSILYCLPILFYL"
     gene            complement(845..1195)
                     /gene="scn"
                     /locus_tag="O0293_002813"
     CDS             complement(845..1195)
                     /gene="scn"
                     /locus_tag="O0293_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-A"
                     /protein_id="HCY0281624.1"
                     /translation="MKIRKSILAGTLAIVLASPLVTNLDKNEAQASTSLPTSNEYQNE
                     KLANELKSLLDELNVNELATGSLNTYYKRTIKISGQKAMYALKSKDFKKMSEAKYQLQ
                     KIYNEIDEALKSKY"
BASE COUNT          512 a          203 c          201 g          613 t
ORIGIN      
        1 aattcattgt aaacttttag acaaaaggaa gtaataaaaa cgtgaaattt aaaacaacta
       61 aagaatgtaa aagtaataat atctttaaaa gaagtcaaga aattaataat agagaaagtg
      121 aaaagggttg tttatggggc attagcatgt tgattctact tttcttattg attctgtttg
      181 gaataactgc ttgttcatca agcattcact ttattaatta gattttttta cttggaggta
      241 tcatgtgaag aaccatacaa atataattaa tatcttatta gttatagtca actcattaac
      301 tcattttcta actctaaaca cctcattttt taataattca gcatcggatt tctgttttat
      361 cataggggct atatttttct taatcggaat ttttgttgca atatacggta tgaagcgagc
      421 aacatattgg ttaaacttat tgattttatt taccaatatt ttttattttc tacacttctg
      481 tgtgttactt ttgttaaaat atataggatt taaattattt atttatgaag ggtgtgtatt
      541 gttatttatc taatttatag tctaattgtc tatttcatct gcattataaa ttttttcata
      601 atggctgcac cattcttaat gactaatgct gattatgtat ggacgcctat gactacagtt
      661 acgttattta ttttgagttt gattattttc ttgatattta taaaaacaaa agatgtcgtt
      721 catttaacaa ttttcatatt aaacttactt ttttcaatac tttattgttt gcctatactg
      781 ttttatttat gaacactttt atattatcta gaaaattaaa accacccgta aaagggtggt
      841 ttttttaata tttacttttt agtgcttcgt caatttcgtt ataaatcttt tgaagttgat
      901 attttgcttc tgacattttc ttaaagtctt ttgacttaag agcatacatt gctttttgac
      961 ctgaaatttt tatagttcgc ttataataag tgtttaaact tccagtagct aattcattaa
     1021 catttagttc atctaataac gattttaatt cattagctaa cttttcgttt tgatattcat
     1081 tcgatgttgg caagcttgtg ctagcttgtg cctcattttt atctagatta gttactagtg
     1141 gtgatgctaa aacgattgct aaagttcccg caagtataga ttttctaatt ttcatttttt
     1201 attctcctta gtaattagta ctaatttatt ttaatgactg actaaaaaaa agtacattaa
     1261 ttattcatta actatataga tcttcttatt aaatgaaaat taataaatta tcaaattgac
     1321 ataccttatt gtatttatag aaaaacaaaa aaaggtaagc accgaaatgc ttacctactt
     1381 cccataaaca atataacaca tatacattga tttggaaagc gcaaaaataa atttaatata
     1441 ctagcccgaa ggggagcaat acataaaaaa tgaaaggcgc tccttgaaaa cgcccaaggt
     1501 aatattaaca taaaatggct actattgca
//
LOCUS       DAKTIO010000298         1509 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.308, whole genome shotgun sequence.
ACCESSION   DAKTIO010000298 DAKTIO010000000
VERSION     DAKTIO010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.308"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            217..828
                     /locus_tag="O0293_002814"
     CDS             217..828
                     /locus_tag="O0293_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HCY0281625.1"
                     /translation="MEFLLLIVVAGLYYIIYLTAVMYSEKIVVLPIIIYAIVFVIIGI
                     TYIFIGDSYDQLTNFNVILYMGSLFYAWMAIRNLWNRPLLLKYKNITDSSSGIVNKSE
                     YNSVESLRINIEIAKYKGIISLIVAIVLTVLMTLKSTPQITAETRDLSISFFILSLFI
                     IIIFAVWDLFIRVRKGAFAFVVIRPILFSCWLFILNMILSRLL"
     gene            964..1182
                     /locus_tag="O0293_002815"
     CDS             964..1182
                     /locus_tag="O0293_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281626.1"
                     /translation="MENQKQGNGLKIATWVFIVLTVVTPLFGIGSIVCSINYKKYDAE
                     KGSKLLQIAIIVTIIAFVLNLLAYLGLR"
     gene            1314..>1509
                     /locus_tag="O0293_002816"
     CDS             1314..>1509
                     /locus_tag="O0293_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0281627.1"
                     /translation="MTFEEKLSEMYSEIANKISSMIPVEWEKVYAMAYIDDGGGEVFY
                     YYTEPGSNELYYYTSVLNKYD"
BASE COUNT          552 a          147 c          268 g          542 t
ORIGIN      
        1 ggcatttcaa tttattatgc aatacagtag attctatatt tttgaattaa acactgaaga
       61 tgatgactaa acaattttat aaattctgtg aagtatactt atatataaga taatcaactt
      121 caacgtatat caccatatta ttttatactc aatgatgtga atggatttaa gtggattgat
      181 attaaagtaa aagttatgga atattgaggt gtgaatatgg agttcttatt attaattgtc
      241 gtagccggac tgtattatat tatatattta actgctgtga tgtattctga aaaaatagta
      301 gtattgccta taatcatcta tgccattgtg tttgtaataa ttggtatcac ttatatcttt
      361 ataggcgaca gctatgatca attaacaaat ttcaatgtga ttttgtatat ggggagtttg
      421 ttttatgcat ggatggctat tagaaatctt tggaacagac cactattact aaaatataag
      481 aacattacag atagttcaag tggaatagtt aataaatctg aatacaattc agttgaaagc
      541 ttacgtataa atattgaaat agctaagtat aaagggatta tttctttgat agtagctata
      601 gtactaacgg tattaatgac attaaagtca acacctcaaa tcactgcaga aacacgtgac
      661 ttaagtatct catttttcat actcagctta tttatcatta ttatatttgc agtttgggat
      721 ttgtttatta gagttagaaa aggagcgttt gcttttgttg taataaggcc aatattattt
      781 agttgttggt tatttatttt gaatatgatt ttatcaagat tattataatt gtattacata
      841 taggtgtggg taaattttta tttatgaaat gataaaaaag tatatactat taatatcttt
      901 tttactataa ttataaatag ttaaataaat tgaaagacaa aataatgagt aggagttaat
      961 gagatggaaa accaaaaaca aggcaatggc ttaaaaattg caacatgggt atttattgta
     1021 ttaacagtag ttacaccgct atttggtatt ggaagtattg tttgtagtat taattataaa
     1081 aaatacgatg ctgaaaaagg ttcgaagtta ttgcaaattg caattatcgt aacaataatt
     1141 gcttttgttt taaatttatt agcatattta ggtttaagat aagtaaaacg aatttgaaga
     1201 agcattaagc gacatttggg tgttgtttaa tgctttttat ttaacggaaa taagtgtaga
     1261 gaataaatta atagtttaat caagagatat ttcgaacaca taggggcgat aacatgactt
     1321 tcgaagaaaa attaagtgaa atgtatagcg agattgcgaa taagattagc agcatgatac
     1381 cggtagagtg ggaaaaggta tatgcaatgg catatataga tgacggagga ggagaagtgt
     1441 tctactatta cacagaacct ggaagtaatg aattatacta ctatactagt gtattaaata
     1501 aatatgata
//
LOCUS       DAKTIO010000299         1504 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.309, whole genome shotgun sequence.
ACCESSION   DAKTIO010000299 DAKTIO010000000
VERSION     DAKTIO010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.309"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..139
                     /locus_tag="O0293_002817"
     CDS             <1..139
                     /locus_tag="O0293_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HCY0281628.1"
                     /translation="VNCPSLPTNVKKEHWGFDDPAGKPWSEFQRVRDEIKIAIENFKS
                     R"
     gene            complement(434..1288)
                     /locus_tag="O0293_002818"
     CDS             complement(434..1288)
                     /locus_tag="O0293_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HCY0281629.1"
                     /translation="MNKHKKGSIFGIIGLVVIFAVVSFLFFSMISDQIFFKHVKSDIK
                     IEKLNVTLNDAAKKQINNYTSQQVSNKKNDAWRDASATEIKSAMDSGTFIDNEKQKYQ
                     FLDLSKYQGIDKNRIKRMLVDRPTLLKHTDDFLKAAKDKHVNEVYLISHALLETGAVK
                     SELANGVEIDGKKYYNFYGVGALDKDPIKTGAEYAKKHGWDTPEKAISGGADFIHKHF
                     LSSTDQNTLYSMRWNPKNPGEHQYATDIKWAESNATIIADFYKNMKTEGKYFKYFVYK
                     DDSKHLNK"
BASE COUNT          485 a          255 c          208 g          556 t
ORIGIN      
        1 cgtaaattgc ccttctttac caacaaatgt taagaaagaa cattggggat ttgatgatcc
       61 tgcaggcaag ccttggtcag agttccaacg tgtaagagat gaaattaaaa tcgcaattga
      121 aaatttcaaa tcacgatgag agtgggacag aaatgataaa aaaacactaa tgatttatta
      181 tgtagtggtt ctttatcatt agctataact aatgtgtact taaaaatagg aatacatgag
      241 taaaactcat gcataagaaa tactaatttc taaagaaaaa gtaattcttt atcgatgtcc
      301 tgccccactt gcattggtgg gattatcaat caatgtgttt tctctaatgc aaaaagacgc
      361 tttctatcaa aacaagatag aaagcgtctt tttagtatgt ccgagtcata aaaacaactc
      421 gtagcttatc aaattactta ttcaaatgtt tactgtcatc tttatacaca aagtatttga
      481 agtattttcc ttcagtcttc atgttcttat aaaagtcagc gataattgtt gcattacttt
      541 ctgcccactt aatatctgta gcatattgat gttctcctgg attttttgga ttccatctca
      601 tactatacaa tgtattttga tctgtgcttg ataagaagtg cttatgaatg aaatcagcac
      661 cgcctgaaat agctttttca ggtgtatccc aaccatgctt tttagcatat tctgcacctg
      721 ttttaattgg gtctttatca agggctccta ctccatagaa attgtagtac tttttgccat
      781 caatttcgac tccattagct aattcacttt taactgcgcc agtttctaat aatgcatgtg
      841 aaattaaata aacttcgtta acgtgcttat ctttagcagc ttttaagaaa tcatccgtat
      901 gtttcaataa cgttggtcta tctactaaca tacgtttaat tctattttta tcaatccctt
      961 gatactttga taaatctaaa aattgatatt tttgcttttc attatcgata aaagtaccgc
     1021 tatccattgc acttttaatt tcagttgcag atgcatctct ccatgcatca ttctttttat
     1081 ttgatacctg ttgactcgta taattattta tttgtttctt tgctgcatcg tttaatgtaa
     1141 catttaactt ttcaatctta atgtcggatt taacatgttt gaaaaatatc tgatcggata
     1201 tcattgagaa aaataaaaat gagacaacag caaatatgac aacaagtcct attattccaa
     1261 aaatagaacc tttcttgtgt ttattcatat ccacacctct taggtcattg ttgttataca
     1321 ttcagtttga ctacgataat aaagtttaac attattaaaa ccgtttaaca attttatcta
     1381 tgcattgtta catattcata aataaacagt aatgtaaatg aaacgtttta aaccatttat
     1441 caacattact ttacgttgat ctacgaatca taaatgtttt atctttttgt ataacctttg
     1501 aaaa
//
LOCUS       DAKTIO010000300         1503 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.310, whole genome shotgun sequence.
ACCESSION   DAKTIO010000300 DAKTIO010000000
VERSION     DAKTIO010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.310"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..575)
                     /locus_tag="O0293_002819"
     CDS             complement(<1..575)
                     /locus_tag="O0293_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0281630.1"
                     /translation="MNLDWYYTFVILAKTLNYRLASEEINLTIPSIHKQIKNLEQHLN
                     VKLFETYKNQIILTEDGHTFLPIAQSFIEQYESGIKHIQLKKTMFQSKLNVVVSSYIA
                     TFIMPKFLKSFFNEHPFIDVSLHVKNENIEKDINNHTYDIGISRNQPKLREVHSEKVC
                     EGKIVLIAPNKENNHLLTEASLFEKYKIISD"
     gene            685..>1503
                     /locus_tag="O0293_002820"
     CDS             685..>1503
                     /locus_tag="O0293_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCY0281631.1"
                     /translation="MIRIQDNTIRDGMQQSNVRKSLIIKKEVLKQINKLNINSVEVGM
                     CTTIEDEFNIHQFRDILSPEKELVVLTRLNEKEIKKIVKLKIHNLVVKILLPISDLHI
                     KEKLNFSNKYYIQKIKDCLDILKKDKKGVDICFEDGTRTSREKLKEYMEIISKYQVRT
                     VTFADTVGCSTPLEYGDIFNYFVKKYSNIIFSAHCHNDLGLATANTLAAILNGAKQIE
                     TTFLRIGERAGNAPIEEIITILTKKQIESTEFTLPDVYKTSINISKILDFQISEN"
BASE COUNT          578 a          179 c          232 g          514 t
ORIGIN      
        1 ttatcactta ttattttgta tttttcaaat aaagatgcct cagttagaag atgattattc
       61 tctttattgg gagcaatcaa tacaatttta ccttcgcata ctttttcaga atgaacttct
      121 cttagtttag gttgatttct gctaatcccg atgtcatacg tatgattatt aatatctttt
      181 tcaatatttt catttttgac atgaagagaa acatcgataa aaggatgctc gttaaagaaa
      241 gattttaaaa atttgggcat aatgaatgtc gcgatatatg aagacacgac aacatttaat
      301 ttcgattgaa acatcgtttt tttaagttgg atatgtttga tgccactttc atattgttca
      361 ataaaacttt gcgcaattgg aagaaatgta tgaccatctt cagtcaagat aatttgattt
      421 ttataagttt caaatagttt cacattgaga tgctgttcta aatttttaat ttgcttatgt
      481 atagaaggta tagtgagatt aatttcttca ctagctaatc gatagtttaa cgtcttcgct
      541 aaaatgacaa atgtatagta ccaatctaaa ttcatgaact tacctctttc aaaaaagtta
      601 ataattcatt agataatatt taatttttat tgcataaaaa taatattaat attatatcag
      661 aggttatcga gggggtgaag taatatgatt cgaattcaag ataatacaat aagggatggt
      721 atgcaacaaa gtaatgttcg aaaaagtcta attataaaaa aagaagtatt gaaacaaatt
      781 aacaagttaa atataaattc tgttgaagta ggcatgtgta caactatcga ggatgaattt
      841 aatattcatc aattcagaga cattttaagt cctgaaaaag aattagtagt attgactagg
      901 cttaatgaaa aagaaataaa aaaaatagtc aaattgaaaa ttcataattt agtggtaaaa
      961 atactattgc caatatctga cttgcatata aaagaaaagc ttaatttttc aaataaatat
     1021 tatattcaga aaatcaaaga ctgcttggat atattaaaga aagataaaaa aggagtagat
     1081 atttgttttg aagatggaac aaggacttct agagaaaaat tgaaagaata catggaaatt
     1141 atttcaaaat atcaagttag aacagttaca tttgcggaca ctgtaggatg ttcgacacca
     1201 ttagaatacg gagatatttt taattacttt gtaaaaaaat attctaacat aattttttct
     1261 gctcattgtc ataacgatct agggttggct actgcaaata cattagctgc aattttaaat
     1321 ggtgcaaagc aaatagaaac tacatttttg agaattggtg agagagcggg taatgctcct
     1381 attgaggaaa taattactat tttgacaaaa aaacaaatag aaagtacgga attcacttta
     1441 cccgacgtat ataaaactag tattaatatt tctaaaattc tcgattttca aatatcagaa
     1501 aac
//
LOCUS       DAKTIO010000301         1479 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.311, whole genome shotgun sequence.
ACCESSION   DAKTIO010000301 DAKTIO010000000
VERSION     DAKTIO010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.311"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            118..1461
                     /gene="brnQ"
                     /locus_tag="O0293_002821"
     CDS             118..1461
                     /gene="brnQ"
                     /locus_tag="O0293_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCY0281632.1"
                     /translation="MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFV
                     LTGIGLPLLGVIVGALDKEGYIGALNKISPKFSILFLIIIYLTIGPLFAIPRTASTSF
                     EMTITPIIHSNSSIALFIFTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMII
                     KGYLDFSGNSAGKGNEALYHSNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGI
                     TKTNQIFKQTLTAGLIAAVALIFIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLT
                     TMASTGFGSFGKYLLGIIVALACLTTACGLIVAVSEYFHRIVPKVSYKAFVLVFILMS
                     FIIANQGLNAVISMSIPVLSIVYPVAITVVLLILIAKFIPTKRISQQIPVIIVFILSI
                     FSVISKLGWLKINFIESLPLRAYSLEWFPVAIIATILGYLVGIFVKQDPIKYQQE"
BASE COUNT          462 a          225 c          241 g          551 t
ORIGIN      
        1 aacacatgtg ttttgtttct gctatgtaca acatatttca tatagactct ttattatttt
       61 ttgtgctaaa atatttactt gtaaattatt tcgtgtttta aaaaaaggag taactctatg
      121 aataaaaata catgggtcat tgggtttacg ctcttcgcta tgttttttgg cgcaggcaat
      181 ctaatttttc ctcctaatct cggattggat agtggtcaat ttttctggcc cgccatttta
      241 gcgtttgttc taactgggat tggtttacca ttattaggtg tgattgtagg tgcacttgat
      301 aaagaaggat atattggcgc attaaataaa atttcaccta aattttcaat attgttctta
      361 atcatcattt atttgactat aggaccactt tttgcaatac ctagaactgc atctacatct
      421 tttgaaatga caattacacc aattatacat agcaatagta gtatcgcttt atttatattt
      481 acgattatct acttcatagt cgttttgtat atttgtttaa atccatctaa gttaatcgat
      541 cgtattggtt cattattaac accattatta ttgattacta ttttagcgat gattattaaa
      601 ggatacttag actttagcgg taatagtgct ggaaagggca atgaagcact atatcattct
      661 aatttttcaa gttttgctga aggctttaca caaggctatt taacaatgga tgccattgca
      721 gcaattgctt tttcaatgat tgttgttaat gcagtaaaac taacaggcat tactaaaaca
      781 aatcaaatat tcaaacaaac tttgactgct ggtttaattg cagccgtagc tttaattttc
      841 atatatattt cattaggtta tattggtaat catatgccag taagtgacat gacgttagat
      901 caattgaaat ccaaagatcg aaacattggg acatatttat taacgacaat ggcttcaaca
      961 ggatttggtt cattcggaaa atatttattg ggcatcattg tggcgctggc atgtctaact
     1021 acagcatgcg ggcttattgt tgcagtttct gaatatttcc atagaatcgt acctaaagta
     1081 tcatacaaag catttgtatt agttttcatt ttaatgagtt ttattattgc taaccaaggt
     1141 ttaaatgctg ttatctcaat gtcaattccg gtattaagca ttgtataccc agtagcaata
     1201 actgttgtat tattaatttt aattgccaaa ttcataccga caaaacgcat ttcacaacaa
     1261 attccagtta ttatcgtatt tatattgtcg attttcagtg ttattagtaa gttaggttgg
     1321 ctgaaaatta actttataga atcattgcct ctaagagcgt attctttaga gtggttccca
     1381 gtagcaatta ttgcaacgat attaggctat ctagtcggca tatttgtaaa acaagatcca
     1441 attaaatatc aacaggaata acgaataata taaaagagg
//
LOCUS       DAKTIO010000302         1478 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.312, whole genome shotgun sequence.
ACCESSION   DAKTIO010000302 DAKTIO010000000
VERSION     DAKTIO010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.312"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            219..725
                     /locus_tag="O0293_002822"
     CDS             219..725
                     /locus_tag="O0293_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281633.1"
                     /translation="MIIKILTILLLLCILSYLVTNRKKPFLFLKTLFMGVVFIFIGYI
                     SLAISAVIIYGIIQFITIDFGSFFLMGIILILISSIFQLFIVRLLFRKKNVDLTEVVV
                     LEHLIQWFLVYFAIYQAVNEKMDINDINIDNFQSVFFDVSNLNLVILPTLIISWVTIF
                     NYRMRSYK"
     gene            984..>1478
                     /locus_tag="O0293_002823"
     CDS             984..>1478
                     /locus_tag="O0293_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding protein"
                     /protein_id="HCY0281634.1"
                     /translation="MKNKLIAKSLLTLAAIGITTTTIASTADASEGYGPREKKPVSIN
                     HNIVEYNDGTFKYQSRPKFNSTPKYIKFKHDYNILEFNDGTFEYGARPQFNKPAAKTD
                     ATIKKEQKLIQAQNLVREFEKTHTVSAHRKAQKAVNLVSFEYKVKKMVLQERIDNVLK
                     QGLVK"
BASE COUNT          559 a          176 c          230 g          513 t
ORIGIN      
        1 attaatatat aagttaaaaa ttatactatt taattgaatt atgcattttc gaagtaaaca
       61 taattcaaca cttacatttt taaatagata aaatagtgta aaatcacgtg ccaagattac
      121 tatagagtag gcattgagta ttattggcaa tcacttaagt gattgttatt tttcgtggtc
      181 aataataaag atataaataa atatattgag gtcaaacgat gataattaaa attttaacaa
      241 ttctattact actttgtata ttgagctatt tggttacaaa tagaaagaag ccttttctgt
      301 tcttaaagac actctttatg ggtgtggtat ttatctttat aggatatatt tcactggcaa
      361 tatctgccgt aattatttat ggtattattc aatttatcac aattgatttt ggtagttttt
      421 tcttaatggg tattatattg atcttgattt caagtatatt ccaattattt atagttagat
      481 tactttttag aaaaaagaat gtcgatttga cagaggttgt cgttttagag catttaattc
      541 aatggttctt agtttacttt gcgatctatc aagcagtaaa tgaaaaaatg gacattaatg
      601 atattaatat cgacaatttc caatctgtct tttttgacgt gtctaatttg aatttagtaa
      661 ttctaccaac gttaatcatt agctgggtca caatatttaa ctatagaatg agaagttaca
      721 aataaaatct atgagattat accttcagac accaacattc aaatggtgtc ttttttgttg
      781 tgtggtttta tttttgaaat tcgaaaaagt agaggcatga attttttgac tagtgtataa
      841 gtgctgatga gtcacaagat agatagctat attttgtcta tattataaag tgtttatagt
      901 taattaataa ttagttaatt tcaaaagttg tataaatagg ataacttaat aaatgtaaga
      961 taataatttg gaggataatt aacatgaaaa ataaattgat agcaaaatct ttattaacat
     1021 tagcggcaat aggtattact acaactacaa ttgcgtcaac agcagatgcg agcgaaggat
     1081 acggtccaag agaaaagaaa ccagtgagta ttaatcacaa tatcgtagag tacaatgatg
     1141 gtacttttaa atatcaatct agaccaaaat ttaactcaac acctaaatat attaaattca
     1201 aacatgacta taatatttta gaatttaacg atggtacatt cgaatatggt gcacgtccac
     1261 aatttaataa accagcagcg aaaactgatg caactattaa aaaagaacaa aaattgattc
     1321 aagctcaaaa tcttgtgaga gaatttgaaa aaacacatac tgtcagtgca cacagaaaag
     1381 cacaaaaggc agtcaactta gtttcgtttg aatacaaagt gaagaaaatg gtcttacaag
     1441 agcgaattga taatgtatta aaacaaggat tagttaaa
//
LOCUS       DAKTIO010000303         1469 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.314, whole genome shotgun sequence.
ACCESSION   DAKTIO010000303 DAKTIO010000000
VERSION     DAKTIO010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.314"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..185
                     /locus_tag="O0293_002824"
     CDS             <1..185
                     /locus_tag="O0293_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCY0281635.1"
                     /translation="DKLLVKTLFIDEIKRQLTYYDETIATNNADRLYFLKQHTLVTEL
                     KYDEIENVYRIKGFKK"
     gene            204..1442
                     /locus_tag="O0293_002825"
     CDS             204..1442
                     /locus_tag="O0293_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCY0281636.1"
                     /translation="MKDISKIVADVESTLAPYFKEIEETAYINQEKVLNAFHHVKATE
                     SDLQGSTGYGYDDFGRDHLEEIYAQAFKAEDAIVRPQIISGTHAITIALQSLLKHGDE
                     LIYITGSPYDTLLEVIGVNGNGIESLMEHGVSYKDIALKEGKIDIESVLDGVSERTKV
                     IAIQRSKGYDQRPSIPLDEIEKVITRLKNVHPNILIFVDNCYGEFVERREPIECGADL
                     IAGSLIKNPGGGLAKIGGYIAGRKDLIERCGYRLTAPGIGKEAGASLNALLEMYQGFF
                     LAPHVVSQSLKGALFTSLFLEKMNMNTTPKYYEKRTDLIQTVKFETKEQMISFCQSIQ
                     HASPINAHFSPEPSYMPGYEDDVIMAAGTFIQGSSIELSADGPIRPPYEAYVQGGLTY
                     EHVKIAVTRAVNQLKEQGLI"
BASE COUNT          536 a          198 c          292 g          443 t
ORIGIN      
        1 gtgataaatt acttgttaag acgttattta ttgatgaaat caaaaggcaa ttaacttatt
       61 atgatgagac aattgcgacg aataatgcag atcgattata ttttctaaaa caacatacat
      121 tagtgactga acttaaatat gatgaaattg aaaatgttta tcgtataaaa ggatttaaaa
      181 aataataaaa ggacgaaatt caaatgaaag atataagtaa gatagtagct gacgtcgaat
      241 caacgttagc accatatttt aaagaaattg aagaaacagc atatattaat caagaaaaag
      301 tattaaatgc atttcatcat gtcaaagcaa ccgaaagtga tctacaagga tcaacaggat
      361 acgggtatga tgactttgga cgtgatcatt tagaagaaat atatgcgcag gcatttaaag
      421 cagaagatgc aattgttcgt ccgcaaatta tttcaggtac gcatgcgatt actattgcat
      481 tacaaagttt attaaaacat ggtgatgaat taatttatat aacgggtagt ccatatgaca
      541 ctttacttga agtcattggc gtaaacggaa atggtattga aagtttaatg gagcatggcg
      601 tatcgtataa agatattgca cttaaagaag gtaagatcga tattgaaagt gtgttagatg
      661 gggtttctga gcgcaccaaa gtaatagcga ttcaacgttc gaaaggctat gatcaaagac
      721 cttcaattcc gctagatgaa attgaaaagg taattactag gttgaaaaac gtgcatccta
      781 atattttaat atttgtggat aactgttatg gggaatttgt tgaaagacgt gaacctatag
      841 aatgtggtgc cgatttaata gcaggatcat taattaaaaa ccctggcggt ggtttagcta
      901 agattggtgg atacattgct ggtagaaaag atttaattga acgatgtggt tatagattga
      961 cagcacctgg tattggtaaa gaagcgggtg catcattaaa tgcattgctt gaaatgtatc
     1021 aaggtttctt tttagcacca cacgttgtca gtcagagtct taaaggtgca ttgtttacta
     1081 gtttattttt agaaaaaatg aatatgaaca caacgccgaa gtactacgaa aaacgaactg
     1141 atttaattca aacagttaaa tttgaaacga aagaacaaat gatttcattt tgtcaaagta
     1201 ttcaacacgc atccccaatt aatgcacatt ttagtccaga acctagttat atgcctggtt
     1261 acgaagatga tgttattatg gcagctggta cgtttattca aggttcatcg attgaattat
     1321 ctgcagatgg acctattcgt cctccttatg aagcatatgt tcaaggagga ttaacatatg
     1381 aacacgttaa aattgctgtg acaagagctg ttaatcagtt gaaagaacaa ggacttatat
     1441 aatagattaa atttcaccct aaatgataa
//
LOCUS       DAKTIO010000304         1455 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.315, whole genome shotgun sequence.
ACCESSION   DAKTIO010000304 DAKTIO010000000
VERSION     DAKTIO010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.315"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            487..591
                     /locus_tag="O0293_002826"
     CDS             487..591
                     /locus_tag="O0293_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281637.1"
                     /translation="MFKVNYSILSYYPEYNIAVSWQRLREGKTIKNKI"
     gene            720..971
                     /locus_tag="O0293_002827"
     CDS             720..971
                     /locus_tag="O0293_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281638.1"
                     /translation="MLTLIKLKEDEQVIIYEYIPEDDISNGKGSVTFNKKDAEVIDFS
                     LSEIENEEYFMLYRNKSFSVVRDFIEKQEFPENYKIAWY"
     gene            1047..1295
                     /locus_tag="O0293_002828"
                     /pseudo
     CDS             1047..1295
                     /locus_tag="O0293_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
BASE COUNT          577 a          165 c          248 g          465 t
ORIGIN      
        1 gctatgttta tttgatttaa ctattaataa aaatcatatg gtgaatggat attataataa
       61 ttaaaataca aaaatagtag attccaattt gtgaaaaagg agtatgtcgt ttcatttaga
      121 atttattgca cgaaattagc atcgaataag ggaaaataag tgttaagttt taaatgataa
      181 aaaagattgg aatggatcgt cttgaaatgc tcccttcaaa gttttcattt tttcaatgtc
      241 gacttcgaag ggggcatttt cattaaattg ttatagcttt ttatatttgt ataatgaaca
      301 tataagttta agaaggtgcg agtgaaggaa ataaaaaagc tcaaatgtac caaattgtta
      361 atcttaataa atctctactt tataaagatt gaatggacat tcgagcgtta atcagtcagg
      421 agggactttc cctcctacaa tttaataata atacttgctt caccactata caaggagtga
      481 gttgttatgt tcaaagtgaa ttattcgatt ttaagttatt atccagaata taatatcgca
      541 gtaagttggc aacgtttaag agaaggaaaa acaataaaaa acaagattta atactgctgc
      601 gtcatgaggc gcttgaacat tatttgatga ataagtataa tttcaactat gattatgcac
      661 ataaaattgt atcaaaaaaa tacgattatt caatttttat aaaaaagaag gtggattaaa
      721 tgcttacatt aataaaattg aaagaagatg aacaggttat aatatatgaa tatatacctg
      781 aagatgatat aagtaacggt aaaggttcag taacttttaa taaaaaagat gcagaggtta
      841 tagatttctc attatctgaa atagaaaatg aagaatattt tatgttatat cgtaataagt
      901 ctttttctgt agtaagagac tttatcgaga agcaagaatt tcccgaaaat tataaaatag
      961 catggtatta aagcatcctt tctacacaga taaagagaaa gcgatgctat ttttatatgc
     1021 ttttttcatt attcgttgaa aaagaagagt cacaaaagtt aacatattta aaagaacttg
     1081 gtgaagatgg cgaatataaa tatgttgcca aaatagatag taaaacatct aaattatgtc
     1141 attcactcaa cggaaaaata tttaaagtta aagatatgat accaggtgtg aatgcgccac
     1201 ctatgcatcc ttggtgtaga agtaccacag tgccacatgt cggcaattgg cgagacaagt
     1261 tctttaaaga gcgtgaaggt aaatatcaag tagaaggttg ttttattgaa tcaggtgcac
     1321 ttaataataa aagtgatgaa tacggtatca aaaggaacag acatgctcaa atttattata
     1381 attcagttag aaatcgagat aaacagatag agatttctaa aattgctaaa aatactaata
     1441 taaataaaaa tttaa
//
LOCUS       DAKTIO010000305         1448 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.316, whole genome shotgun sequence.
ACCESSION   DAKTIO010000305 DAKTIO010000000
VERSION     DAKTIO010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.316"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            307..708
                     /locus_tag="O0293_002829"
     CDS             307..708
                     /locus_tag="O0293_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl peptide receptor-like 1 inhibitory
                     protein"
                     /protein_id="HCY0281639.1"
                     /translation="MKKNITKTIIASTVIAAGLLTQTNDAKAFFSYEWKGLEIAKNLA
                     DQAKKDDERIDKLMKESDKNLTPYKAETVNDLYLIVKKLSQGDVKKAVVRIKDGGPRD
                     YYTFDLTRPLEENRKNIKVVKNGEIDSITWY"
     gene            668..760
                     /locus_tag="O0293_002830"
     CDS             668..760
                     /locus_tag="O0293_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281640.1"
                     /translation="MLKTVKSTRLLGIKKHTELNSCTPNVRKQC"
     gene            complement(1077..1406)
                     /gene="ecb"
                     /locus_tag="O0293_002831"
     CDS             complement(1077..1406)
                     /gene="ecb"
                     /locus_tag="O0293_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Ecb"
                     /protein_id="HCY0281641.1"
                     /translation="MKKNFIGKSILSIAAISLTVSTFAGESHAQTKNVEAAKKYDQYQ
                     TNFKKQVNKKVVDAQKAVNFFKRTRTVATHRKAQRAVNLIHFQHSYEKKKLQRQIDLV
                     LKYNTLK"
BASE COUNT          514 a          217 c          194 g          523 t
ORIGIN      
        1 ttgtctatcc aacttagcaa acaaaatgta gagaaatgta aatatcgttt tagcaataaa
       61 aattattcaa tcatagttga gtgacgttta ctactattat tatactatca acaaattcaa
      121 taatacctca aataaaaata ataccatcaa attcatcatt attctttttc gattgcatta
      181 aaccttatta atttgcactt aattttaatt aatgaagtgt taactggtaa atgtatgaaa
      241 ccgtttgtaa aatagcagtg tactcgcatt agtaagtaca aatttatttt acagggagaa
      301 cttattatga aaaaaaatat cacaaaaact attatcgcat ctacagtaat cgctgcaggt
      361 cttttaactc aaactaatga tgctaaagct ttctttagtt atgaatggaa aggtttagaa
      421 atcgcaaaaa atttagcaga tcaagctaaa aaggacgatg aacgtattga taagttaatg
      481 aaagagtctg ataaaaatct aactccttac aaagctgaaa ctgttaatga tctgtacctt
      541 attgttaaaa aattaagcca aggtgatgtg aagaaagcag ttgtcagaat taaagatggt
      601 ggtcctagag attactatac ttttgactta actcgtcctt tagaagagaa cagaaaaaat
      661 attaaagttg ttaaaaacgg tgaaatcgac tcgattactt ggtattaaaa aacatactga
      721 attaaatagt tgtacgccaa acgttagaaa acaatgctaa cgtattggcg tgctttttta
      781 ttttaagtaa cttccaattt atttagcatc tttacgactg tttaataaag cacgtatgat
      841 taacacggtt gccactatat ccgttacaat ttttatgatt gttaatacat tcgatttcct
      901 tttcacaata gccacctcgc ttgttcaaaa cataaacaac tattgcattc acttttaagt
      961 aattatttat attatttatc ccaataagct caccattcaa ataaacacaa tattataaaa
     1021 taattatacc tttggatata gcaaaaagcc acactctata gcatggcttc tatcaattat
     1081 tttaaagtat tatattttaa aactagatcg atttgtcttt gtaatttttt cttttcataa
     1141 ctgtgttgga aatgaattaa attaacagct ctttgtgctt tacggtgtgt tgcaacagtt
     1201 cttgtacgtt tgaaaaagtt tacagctttt tgtgcatcca caactttttt atttacttgt
     1261 tttttaaagt ttgtttgata ctgatcatat tttttagcag cttcaacgtt tttagtttgt
     1321 gcatgagatt caccggcaaa cgttgatacc gttaaactaa tagcagctat gcttaaaatt
     1381 gatttcccaa taaaattctt tttcataatt aataacacct atcctcaaat atttattttg
     1441 tatatatt
//
LOCUS       DAKTIO010000306         1446 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.317, whole genome shotgun sequence.
ACCESSION   DAKTIO010000306 DAKTIO010000000
VERSION     DAKTIO010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.317"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            65..1009
                     /gene="whiA"
                     /locus_tag="O0293_002832"
     CDS             65..1009
                     /gene="whiA"
                     /locus_tag="O0293_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCY0281642.1"
                     /translation="MSFASEMKNELTRIDVDEMNAKAELSALIRMNGALSLSNQQFVI
                     NVQTENATTARRIYSLIKRVFNVEVEILVRKKMKLKKNNIYICRTKMKAKEILDELGI
                     LKDGIFTHEIDHSMIQDDEMRRSYLRGAFLAGGSVNNPETSSYHLEIFSQNESHAEGL
                     TKLMNSYELNAKHLERKKGSITYLKEAEKISDFLSLIGGYQALLKFEDVRIVRDMRNS
                     VNRLVNCETANLNKTVSAAMKQVESIKLIDKEIGIENLPDRLREIARIRVEHQEISLK
                     ELGEMVSTGPISKSGVNHRLRKLNDLADKIRNGEQIEL"
BASE COUNT          558 a          185 c          281 g          422 t
ORIGIN      
        1 aaatgaaaat agacagtggt tctaaggtga atcatgtttt aaataagaaa ggaatgactg
       61 tacgatgagc tttgcatcag aaatgaaaaa tgaattaact agaatagacg tcgatgaaat
      121 gaatgcaaaa gcagagctca gtgcactgat tcgaatgaat ggtgcactta gtctttcaaa
      181 tcaacaattt gttataaatg ttcaaacgga aaatgcaaca acggcaagac gtatttattc
      241 gttgattaaa cgtgtcttta atgtggaagt tgaaatatta gtccgtaaaa aaatgaaact
      301 taaaaaaaat aatatttata tttgtcgtac aaagatgaaa gcgaaagaaa ttcttgatga
      361 attaggaatt ttaaaagacg gcatttttac gcatgaaatt gatcattcaa tgattcaaga
      421 tgacgaaatg agacgcagtt acttgagagg agcttttctg gcaggtggct cagtgaataa
      481 ccctgaaaca tcttcgtacc atttggaaat tttttctcaa aatgagagtc atgcagaagg
      541 cttaacgaaa ctaatgaata gttatgagtt gaatgccaaa catttagagc gaaaaaaagg
      601 aagtattacg tatttaaaag aagcggaaaa gatttcggat tttcttagtt tgataggtgg
      661 ctatcaagcg ttattaaaat ttgaagacgt acgtattgta agagatatgc gtaattctgt
      721 taaccgactc gttaattgtg aaacggccaa tctaaataaa acagttagtg ctgcgatgaa
      781 acaagttgag agcattaaat tgattgataa agaaattggt attgaaaatt taccagacag
      841 gttgagagag attgctagaa ttcgagtaga acatcaagaa atttcgttga aagagcttgg
      901 agaaatggta tcaactggtc caatttcaaa atcaggtgta aatcaccgat taagaaaact
      961 gaatgattta gccgataaga ttagaaatgg tgaacaaata gaattataag taagaaggtg
     1021 tttttggtag tgaattatca aaaacacttt ttttatgatt aaaaagtgtt taatgaataa
     1081 tagtcgacta actattaaat tgagctggaa agtttaatga aggaaattca ataaccaagt
     1141 gatgcctaat tttctcatta aatagcatca tatgtatcaa tttggtcata agaaaatatg
     1201 aatgtgataa ttaattgagt ccctgaaagt ccctgatcat agacaaaaac aaaatcagca
     1261 taaataagaa tcccgacatt gcgggattct ctgtattgaa aagtattgta ttttattaaa
     1321 acagcctcct tgaagggaat tgaaccccta tcttaagaac cggaatctta cgtgttatcc
     1381 attacaccac aaggagcaaa tatgtaatca tatctctgta ctatgagaag taaaataagt
     1441 acaatc
//
LOCUS       DAKTIO010000307         1442 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.318, whole genome shotgun sequence.
ACCESSION   DAKTIO010000307 DAKTIO010000000
VERSION     DAKTIO010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.318"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..70)
                     /locus_tag="O0293_002833"
     CDS             complement(<1..70)
                     /locus_tag="O0293_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance protein SepA"
                     /protein_id="HCY0281643.1"
                     /translation="MIVNYLKHKFYNLLTTMIVLFIF"
     gene            complement(169..>1442)
                     /locus_tag="O0293_002834"
     CDS             complement(169..>1442)
                     /locus_tag="O0293_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0281644.1"
                     /translation="IISLALPTIKQDLNAGNLISLIFTAYFIALVIANPIVGELLSRF
                     KIIYVAIAGLLLFSIGSFMCGLSTNFTMLIISRVIQGFGSGVLMSLSQIVPKLAFEIP
                     LRYKIMGIVGSVWGISSIIGPLLGGGILEFATWHWLFYINIPIAIIAIILVIWTFHFP
                     EEETVAKSKFDTKGLTLFYVFIGLIMFALLNQQLLLLNFLSFILAIVVAMCLFKVEKH
                     VSSPFLPVVEFNRSITLVFITDLLTAICLMGFNLYIPVYLQEQLGLSPLQSGLVIFPL
                     SVAWITLNFNLHRIEAKLSRKVIYLLSFTLLLVSSIIISFGIKLPVLIAFVLILAGLS
                     FGYIYTKDSVIVQEETSPLQMKKMMSFYGLTKNLGASIGSTIMGYLYAIQSGIFGPNL
                     HNVLSAVAVISIGLIVLWVVFFKEQSSQSKE"
BASE COUNT          563 a          246 c          185 g          448 t
ORIGIN      
        1 caaaaataaa gagaacaatc attgtagtta acaagttata aaacttatgt ttaagataat
       61 tcacaatcat tggcctcctt attaataaaa tattgaacga tttatctagt atctaggaaa
      121 tttattattt cttactttaa ttacactaca tcaattttaa tcataattct attcttttga
      181 ttgagatgac tgttctttaa aaaatacgac ccataaaaca ataagcccaa tgctaattac
      241 agcaacagca cttaacacat tgtgtaagtt tggaccaaag attcctgatt gtatcgcata
      301 aagatatccc attattgttg aacctattga tgccccaaga tttttagtta atccataaaa
      361 tgacatcatt ttcttcattt gtaatgggct agtttcctct tggacaatca cactatcttt
      421 cgtataaata tatccaaaac ttaatcctgc caaaattaac acaaatgcta taagtaccgg
      481 caatttaata ccaaatgaaa tgataatact acttactagt agcaatgtaa atgatagtaa
      541 gtaaataact ttccttgata gttttgcttc aattcgatgt aaattaaaat tcaatgtaat
      601 ccaagctaca gataaaggaa aaataaccaa tccactttgc aatggagata atcctagttg
      661 ttcttgaagg tagactggaa tatataaatt gaatcccatt aaacaaatag ctgttagaag
      721 gtcagttata aaaactaaag taatcgaacg attaaattca accacaggta aaaatggtga
      781 agaaacatgt ttttctactt taaataaaca cattgccaca acgatagcta agataaaact
      841 caagaaattt aaaagtaaca gctgttggtt taataaagca aacataatca atccaataaa
      901 tacataaaat agcgtgagcc ctttagtatc aaattttgat ttagcaactg tttcttcctc
      961 tggaaaatga aatgtccata taactaatat aatggcaatg atcgcaatcg gtatatttat
     1021 gtagaataac caatgccatg ttgcaaattc taatatacca ccacctagaa gtggcccaat
     1081 gatactcgaa ataccccaaa cactgccaac tatgcccata attttatatc gtaacggaat
     1141 ttcaaatgct aactttggaa caatttgcga taatgacatc aatacacctg atccaaaacc
     1201 ttgtatcacg cgtgatataa ttagcatagt aaaattagta cttaaaccac acataaaact
     1261 acctatacta aataaaagta atcctgcaat tgccacataa ataattttaa atctcgaaag
     1321 taattcgccc actataggat tggcaattac taatgcgata aaatacgctg tgaaaattaa
     1381 tgaaattaaa tttcctgcat ttaaatcttg tttaattgta ggtaatgcaa gcgaaataat
     1441 ag
//
LOCUS       DAKTIO010000308         1441 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.319, whole genome shotgun sequence.
ACCESSION   DAKTIO010000308 DAKTIO010000000
VERSION     DAKTIO010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.319"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            70..1431
                     /locus_tag="O0293_002835"
     CDS             70..1431
                     /locus_tag="O0293_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0281645.1"
                     /translation="MAEKLQRELSNRHIQLIAIGGAIGTGLFLGAGQTIALTGPSILL
                     TYIIIGFMLFMFMRGLGEIIIQNTEFKSFADVTNTYIGPFAGFVTGWTYWFCWIITGM
                     AEVTAVAKYVSFWFPEIPNWISALFCVLLLMSFNLLSARLFGELEFWFSIIKIATIIG
                     LIVVGFVMILFAFKTQFGHASFTNLYEHGIFAKGASGFFMSFQMALFSFVGIEMIGVT
                     AGETKDPVKTIPKAINSVPIRILIFYVGALAVIMSIIPWQQVDPDNSPFVKLFALIGI
                     PFAAGLINFVVLTAAASSCNSGIFSNSRMLFGLSSQQQAPPNFSKTNKYGVPHVAIFA
                     SSALLLVAALLNYIFPDATKVFTYVTTISTVLFLVVWGLIIIAYINYSRKNPDLHKNA
                     TYKLLGGKYMGYLIFVFFIFVFGLLFINVDTRRAIYFIPIWFILLAFMYLRYKRIAAK
                     SNK"
BASE COUNT          437 a          236 c          258 g          510 t
ORIGIN      
        1 ccgaaaataa ttgtatatat ctcaatataa gtgcataata aacatatcta tatttaaggg
       61 agatgtatta tggctgaaaa attacaaagg gaactgagca atcgccacat acaattaatt
      121 gcaattggcg gtgcaattgg tacaggctta ttcttaggtg ctggtcaaac gattgcatta
      181 accggccctt caattctatt aacatacatc attataggat ttatgttatt tatgtttatg
      241 cgaggtttag gagaaatcat tatacagaat actgaattta aatctttcgc agatgtaacc
      301 aatacatata ttgggccttt tgcaggattt gttaccggat ggacatactg gttctgttgg
      361 attattacag gtatggctga agtaacggct gtggcaaaat atgttagctt ttggttccca
      421 gaaattccaa actggataag tgcactattt tgtgtactgt tattaatgtc attcaaccta
      481 cttagcgcaa gacttttcgg agaattagaa ttttggttct ctatcattaa aatagcgaca
      541 attattggtt taatagtagt tggtttcgtc atgattctat ttgcatttaa aactcaattc
      601 gggcatgcca gtttcacaaa tctatatgaa cacggcatat tcgctaaagg tgcttctgga
      661 ttctttatgt ctttccaaat ggcactattc tcatttgtag gaattgaaat gattggtgtt
      721 acagctgggg aaacaaaaga tccagttaaa acaattccaa aagcaattaa cagtgtaccc
      781 attagaattt taatatttta cgttggggcg ttagcggtta tcatgtctat tatcccttgg
      841 cagcaagttg atcctgataa cagtccattc gtaaaattat tcgcattgat cggaattccg
      901 tttgctgcgg gcttgattaa ttttgtagta ttaaccgctg ctgcttcatc atgtaacagt
      961 ggtatattct caaatagccg tatgcttttc ggtttatcaa gtcaacaaca agcacctccg
     1021 aacttttcta agacgaataa atatggcgtt ccacatgttg caatctttgc ttcatcagca
     1081 ttattacttg tggcagcatt actaaactat attttcccag atgcgacaaa agtatttacg
     1141 tatgtgacta ccatctctac agtgttattt ttagttgtat ggggtctgat tatcattgca
     1201 tatatcaatt atagtcgtaa aaacccagat ctacataaaa atgctacgta caaactatta
     1261 ggtggcaaat atatgggcta cttaatattt gtattcttca tttttgtgtt cgggttatta
     1321 tttattaatg ttgatacaag acgtgcaatt tattttattc cgatttggtt tatactttta
     1381 gcatttatgt acttaagata taaacgtatc gctgctaaat caaataaata acaacaagtt
     1441 t
//
LOCUS       DAKTIO010000309         1438 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.320, whole genome shotgun sequence.
ACCESSION   DAKTIO010000309 DAKTIO010000000
VERSION     DAKTIO010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.320"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(107..1132)
                     /locus_tag="O0293_002836"
     CDS             complement(107..1132)
                     /locus_tag="O0293_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HCY0281646.1"
                     /translation="MISFENDYLEGAHEKVLKRLVDTNLVQASGYGFDQFTAQAIEKI
                     KDTIDCPNATIRFLVGGTQTNQVVINSMLESYEGVISADTGHVAVHEGGAIEYSGHKV
                     ITIPSKEGKVSASDVETYMETFKSDFKKDHMVFPGMVYISHPTEYGTLYSKSELEELC
                     KVCKQYQLPLFMDGARLGYGLMSDQSDMTIKDIAKYCDVFYIGGTKIGALCGEAIVFT
                     KNNEPKQFTTRIKHHGALLAKGRLTGIQFLELFTDNLYFNISRHAIEMANKMKDGFKN
                     KGYRLYFDSPTNQQFFILSNEKIAELEQKVKFAVWEKYDDQHRVVRFATSWATTEENL
                     NKLLELI"
BASE COUNT          477 a          259 c          194 g          508 t
ORIGIN      
        1 tcatcaacgg ctacaaatga gcagtttcat atataaataa acacatctac tcaactgatc
       61 ttaattaatt aataaatgat ttaccagatg acatataaca gcattattag attaattcta
      121 atagtttatt taaattttct tcggttgtcg cccaactggt tgcgaatcta acaacacgat
      181 gttgatcatc gtatttttcc caaacagcaa atttaacttt ttgttctaac tctgctattt
      241 tctcgttact taaaataaaa aattgttgat tggttggaga atcaaagtaa agacgatagc
      301 ctttattttt aaacccgtct ttcatcttat ttgccatttc gatagcatgt ctgcttatat
      361 taaaatataa attgtccgta aataattcta aaaattgtat gcctgttaac cgtccttttg
      421 ctaaaagggc accgtgatgc ttgattcgag tggtaaattg tttcggttca ttatttttcg
      481 taaaaacaat ggcttccccg cataatgcac ctatcttcgt accacctata taaaatacat
      541 cacaatattt agcgatgtct ttaatagtca tatctgattg gtcactcatc aatccatacc
      601 ctaatcgtgc accatccata aataatggaa gctgatattg cttacatact ttgcataact
      661 cttccaattc tgatttagag tataatgtgc catattctgt aggatgagaa atatatacca
      721 ttcctgggaa taccatatgg tcctttttaa aatcactttt aaatgtctcc atgtaagttt
      781 caacatctga agcactaact tttccttcct tagagggtat agtaattact ttatgtccac
      841 tatattcaat tgcaccgccc tcatgcacag caacatgacc agtgtctgct gaaatgaccc
      901 cttcgtaact ttctaacatt gaattaataa caacctgatt ggtttgtgtt ccacctacta
      961 aaaaacgaat tgtagcattt ggacagtcaa ttgtatcttt aatcttttca attgcctgag
     1021 ctgtgaattg atcaaagcca tatcccgaag cttgtacaag atttgtatct actaatcgtt
     1081 ttaatacttt ttcatgagca ccttctaaat aatcattttc gaatgaaatc actacatttc
     1141 cccctaaaac taatatcaac attttaataa gataaaccaa tttcaaaact agttcgatat
     1201 ttaaaatgta ttatgaatgg ttaaagtttg tatcgcatta tcgcgaagtt gaataaatat
     1261 attatacatc aaacaataca ttatgaaaat taattaattt tttatcacag cgacttttca
     1321 tgctggaaca catgtattaa tgattgaatt ttactgttaa aagttctatg atttaggaaa
     1381 tagagaaaag aggggattta aatggaacaa attaaactta aaacttttac agctgaga
//
LOCUS       DAKTIO010000310         1437 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.321, whole genome shotgun sequence.
ACCESSION   DAKTIO010000310 DAKTIO010000000
VERSION     DAKTIO010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.321"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..1335)
                     /locus_tag="O0293_002837"
     CDS             complement(<1..1335)
                     /locus_tag="O0293_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281647.1"
                     /translation="MKTIQLHKTTLILIAILLFYTFMGILLPLMHDDLQWFSNYNTDI
                     LKVGFASLNGRYIGNIFEIIAVHVSWLRWLSYGLISMGIIWMIMHITRCKAWTSYYLL
                     AFSLMLILPSAIYADTYGWFAGFYNYATSTLISLFIIYYCINAIIYKEKQPVSVTVLF
                     YVLCFFGQLFMENVTLFHCIILILAFLYEFTANRTLNYKLLFSFMIATIGTIIMFSNP
                     NYRKILFEGSEYQQVSNNQGIFSKFAEMISTSLPYGVIFSQIIILSMIAALIIYLLLR
                     SDRYVHLTILKRRIIMIGFITLPLYYLLFYNQFLLNKNTDIGLVGFVNVIVCGYFAFS
                     IFVGIYLSINDRKTQITLYSLLIAICVSASTLVIVTPIEPGNFLIVYTIHVIILIILL
                     KEFRKYKSINVNFIKGASLVLAVIYLSAFIYVHYEHEKRVQLLKQQIKDHPRQ"
BASE COUNT          564 a          216 c          189 g          468 t
ORIGIN      
        1 ttgtcgtgga tgatctttaa tttgttgttt taataactga actctttttt catgttcata
       61 atgaacataa ataaatgcac taagatatat cacagctaaa actagtgatg cacctttaat
      121 aaaattaaca ttgattgact tatatttccg aaattctttt aataaaataa ttaaaatgat
      181 tacatgtatc gtataaacaa tcaaaaaatt acctggttct attggagtaa caatgactag
      241 tgtcgacgcc gaaacacata ttgcaataag taaggaatat aaagtgattt gtgttttacg
      301 atcattaata gacaaataaa tacctacaaa tatcgaaaac gcaaagtaac cacatacaat
      361 tacgttcaca aaaccaacca atccaatatc tgtattttta tttaataaaa actgattgta
      421 aaatagtaaa taataaagcg gtaaagtgat aaatcctatc ataatgatac gacgctttaa
      481 tattgttaaa tgtacatacc gatcacttct aagcagtaga taaataataa gcgctgcaat
      541 catacttaaa attataatct gactgaaaat gacaccatat ggaagtgacg ttgatatcat
      601 ttctgcaaat tttgaaaaga taccttgatt atttgaaact tgttgatatt ctgatccttc
      661 aaataaaatt ttgcgataat ttggattgga aaacataatg attgtaccta tagtagcaat
      721 cataaatgaa aacaataatt tataattaag tgttcgattt gcagtaaatt catacagaaa
      781 agctaaaatt aaaatgatac agtggaataa tgtcacattc tccataaata attgaccgaa
      841 gaagcataaa acatagaata gtacagtaac acttaccggc tgcttttctt tatatataat
      901 tgcattaata caataataaa taataaagag tgaaattagt gttgatgtcg cataattata
      961 aaatcctgca aaccagccat atgtatctgc ataaatagca cttggtaaaa ttaacattaa
     1021 agaaaatgct aacaaataat agcttgtcca cgctttacaa cgtgtaatgt gcataatcat
     1081 ccaaatgatg cccatactaa tgaggccata tgaaagccaa cgtaaccagc ttacatgtac
     1141 agctataatt tcaaagatat ttccgatata gcgaccatta agtgatgcaa atcctacttt
     1201 taaaatatca gtattataat tgctaaacca ttgtaaatca tcgtgcatga gtggtagtaa
     1261 gatacccata aaagtataaa acaataatat cgcaattaat atcaaagttg tcttgtgtaa
     1321 ttgaattgtt ttcactttgc taatcctcaa atctagttaa attttcctca acttgtaggt
     1381 cgaaaaatta attcaatatt tttaatgtat ttctaatttt cacctatgca tgtttcc
//
LOCUS       DAKTIO010000311         1433 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.322, whole genome shotgun sequence.
ACCESSION   DAKTIO010000311 DAKTIO010000000
VERSION     DAKTIO010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.322"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..427)
                     /locus_tag="O0293_002838"
     CDS             complement(<1..427)
                     /locus_tag="O0293_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0281648.1"
                     /translation="MLLCDVRVIYKNPKYKVIQHNGEYLLVDLVSTWFVYFFPFINWF
                     IPKKYAIISEEEFENLNVVKPNKNNVFWSVIGSSVLFGVTLRKYIHVFDVQLDKLVVM
                     ILCALALICVIVFYFNLNRKLKLKVFDTNIEKNKRVILIP"
     gene            complement(880..1433)
                     /locus_tag="O0293_002839"
                     /pseudo
     CDS             complement(880..1433)
                     /locus_tag="O0293_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837136.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF443 family protein"
BASE COUNT          526 a          247 c          149 g          511 t
ORIGIN      
        1 ttggtattaa tataactctc ttattttttt caatatttgt atcaaacact tttaacttaa
       61 gctttctatt taagttaaaa taaaaaacta taacacaaat taaagcgaga gcacacaata
      121 tcattacaac tagcttatct aattgaacat caaaaacatg tatgtatttc cttaaagtaa
      181 ctccaaacaa aaccgaactt cctataactg accagaaaac attattttta tttggtttaa
      241 caacatttaa attttcaaat tcttcttcgc taattatcgc gtactttttt ggaatgaacc
      301 aattaatgaa aggaaaaaag tacacgaacc aagtgcttac taaatcgact aataagtatt
      361 caccgttatg ttgaatgact ttgtatttcg gatttttata aatgactctg acatcgcaaa
      421 gcaacattta ccactcccgt ttcattagtt ttaaatgatc cccaatactt tttgtacctt
      481 caccaactat cgttccaaca ctatctacag ctttttctgt ggtattttta aacagcgtct
      541 atttaatgtc ttatccaatt agttttatct cttaatccaa atttattagt atgaaaagtc
      601 caatttttaa gaattgtcta aacagattcc gcaacaaaac cagctaaaac cgtccatact
      661 attggattaa aaacgccaat gttataagag ctatcataca aaataacacg ctctatattt
      721 catttttaaa ttaaaacagt tttttccttt atcaatcaca atgggcattt tcacttggta
      781 tattcaaaca ttactatcta ttttcctgat ttaagatatg attatatttt tttgaaccac
      841 cgtttaaata tgcgatacta ccaatttcga tcatttttac tactcgttta ttcttaaaat
      901 aacatgaaca tttttattac ctatcgaagc catattcact aaaaagaaaa acatcgtgat
      961 aataacccaa gttaaaaata ttattatatt ttgtgactct gtcgtcaaaa gaatggttaa
     1021 agtaaatatt gaagcgcttc ctaaaaaaaa gtacgcaaaa actattaaca atccttgttt
     1081 gaaagtcggt attaatacaa ctctattctt attaaccacg ttggtattaa aaatttttaa
     1141 tgttaatttt ttatttagat aaaaataaaa aattaacatc cctattaaca tgataaaaaa
     1201 tacagtgatt gctagttgtt tttcaaactg aacattaaag aagttaccgt actttctcaa
     1261 tataattcca aatagaactg aacttcctgc aaccggccaa aaaacattat ttttaacagg
     1321 ctcgactata tttaaccttt aaaattcatt ttcgctaatt tttgcgtatt ttttgggcaa
     1381 aaaccaatta atcataggga aaaaataagt aatccaagta cttactaaat cga
//
LOCUS       DAKTIO010000312         1421 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.323, whole genome shotgun sequence.
ACCESSION   DAKTIO010000312 DAKTIO010000000
VERSION     DAKTIO010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.323"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..>1421
                     /locus_tag="O0293_002840"
     CDS             <1..>1421
                     /locus_tag="O0293_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR04141 family sporadically distributed
                     protein"
                     /protein_id="HCY0281649.1"
                     /translation="SDSNELKLISKVYIRKKDKKLLDWQLLIKNVYLDTEEDDNLFSE
                     SGHHFDAILFLKEDTTLQNNVYIIPFGQAYHDINNLIDYDFGIDFAERAIKNEDIVNK
                     NVNFFQQNRLKEIVNYRRNSVDYVRPSESYISVQGHPQNPQIFGKTMTCGTSISLRVP
                     NRKQQFIDKISVIIKEINAIINLPQKISEFPRIVTLKDLNKIEVLDTLLLKKLSNSST
                     TENISIDISRFLELSNMILLLDDMLDVNIYIYINSFKNNTLETFDATDPEVDYITEIG
                     DYLLKYDVNSINDVRIEVIDNLGHSNNMLLKTILHAEVEMEDGKKYLLQNGKWGYFNR
                     EFFDLLNDHLNEIEIRYNTLTPTGLVFKEGEEGYIKEIVGRLPEEYLMLHKKFIKPIN
                     KNFIVKGNGIELADLYNIKNKELFTIKRGINTSLSLYSLEQNIIAINALKYPESYNFE
                     ELIEAIPDNSENIFNDIQRSTNF"
BASE COUNT          603 a          156 c          213 g          449 t
ORIGIN      
        1 tagtgattca aatgaattaa aattaattag taaagtttat atacgtaaaa aagacaaaaa
       61 actattagat tggcaattat taataaagaa tgtataccta gatactgaag aagatgacaa
      121 tttattttca gaatccggtc atcattttga tgcaatatta tttctcaaag aagatacaac
      181 attacaaaat aatgtatata ttataccttt tggacaagca tatcatgata taaataattt
      241 gattgattat gacttcggaa ttgattttgc agaaagagca atcaaaaatg aagacatagt
      301 taataaaaat gttaattttt ttcaacaaaa caggcttaaa gagattgtta attatagaag
      361 gaatagtgta gattacgtta gaccttcaga atcttatata tcagtccaag gacatccaca
      421 gaatcctcaa atttttggaa aaacaatgac ttgtggtaca agtatttcat tgcgtgtacc
      481 gaatagaaag cagcaattca tagataaaat tagtgtgata atcaaagaaa taaacgctat
      541 tattaatctt cctcaaaaaa ttagtgaatt tcctagaata gtaactttaa aagacttgaa
      601 taaaatagaa gtattagata ctttattgct aaaaaaacta tcgaattctt caactacaga
      661 aaatatatct atagatatat caagattttt agaactaagt aatatgatac tcttgttaga
      721 tgatatgctc gacgtcaata tatatatata tataaattct tttaaaaata atacattaga
      781 gacatttgat gcgactgatc ctgaagttga ttacatcact gaaataggag attacttatt
      841 aaaatatgac gttaattcta taaatgatgt cagaattgaa gtgatagata atttggggca
      901 ttcaaataat atgctactaa aaacgatact acatgcagaa gttgaaatgg aagatggaaa
      961 gaaatattta ttacaaaatg ggaaatgggg ttattttaat agagaatttt ttgacctttt
     1021 gaatgatcat ttgaatgaga tagaaattag gtataacaca ctaactccca caggtttagt
     1081 atttaaagaa ggagaagaag gatatataaa agaaatagta ggaagattgc cagaagaata
     1141 tttaatgtta cataaaaaat tcataaaacc aataaataag aattttatag taaaaggaaa
     1201 tggaatagag ttggcagact tatacaatat taaaaacaaa gagcttttca cgattaaaag
     1261 aggaattaat acatctttat ctctttatag tctagaacag aatataatag caattaacgc
     1321 cttaaaatat ccagaatcat ataattttga agaattaata gaagctattc ctgataactc
     1381 ggaaaatata tttaatgata tacaacggag tacaaatttt a
//
LOCUS       DAKTIO010000313         1420 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.324, whole genome shotgun sequence.
ACCESSION   DAKTIO010000313 DAKTIO010000000
VERSION     DAKTIO010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.324"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..173)
                     /locus_tag="O0293_002841"
     CDS             complement(<1..173)
                     /locus_tag="O0293_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCY0281650.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHH
                     STVYRWVQEYAPI"
     gene            complement(444..539)
                     /locus_tag="O0293_002842"
     CDS             complement(444..539)
                     /locus_tag="O0293_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCY0281651.1"
                     /translation="MLEILVHITTTVISGCIIALFTHWLRNRNNK"
     gene            704..1369
                     /locus_tag="O0293_002843"
     CDS             704..1369
                     /locus_tag="O0293_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001568510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257-2 family transposase"
                     /protein_id="HCY0281652.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHH
                     STVYRWVQEYAPVLYQIWKKKHKKAYYKWRVDETYIKIKGQWCYLYRAIDADGHTLDI
                     WLRKKRDHQSAYAFIKRLIKQFGKPQMIITDQAPSTKVAMAKIIKVFKLKPNCHCTSK
                     YLNNFIEQDHRHIKVRKTRYQSVNTAKNTLKGIECIYGLYKKTVGLFRSTDFHHATKL
                     VIC"
BASE COUNT          491 a          239 c          262 g          428 t
ORIGIN      
        1 aaaattgggg catattcttg aacccaacgg tagaccgttg aatgatgaac gttgacacca
       61 cgttccctta atatttcaga tatatcacga taactcaatg catatcttag atagtagcca
      121 acggctacag tgataacatc tttgttaaat tgtttatatc tgaaatagtt catacagaag
      181 actccttttt gttaaaatta tactacaaaa tcaactttgc aacagaacca gttaaacaat
      241 gttatgccaa gttacaatgt ttttatccgt aaagacgagt tagattatag atagaaataa
      301 tattcaaaag agaaatgaat gaggactttg catttctcca ctcaccgatg ttataattta
      361 gttaacaagg aaactagcat agatgcacca atcccctcac tacggcaata gtgaggggat
      421 tttttaggtg tggctatggt cgcctattta ttattgcgat tacgtagcca atgcgtaaat
      481 aacgcaataa tacaaccact gatgactgtg gtcgtgatgt gaacaaggat ttctagcata
      541 gattagcacc tcctctctgc gtcggaattg acgcctgaga gatagacgac ttgtatatta
      601 tatcatctac gaatatacga tgataggtag cttctgataa gtgatctggt tctgttgcaa
      661 agttgatttt gtagtataat tttaacaaaa aggagtatgc tatatgaact atttcagata
      721 taaacaattt aacaaggacg tcatcactgt agccgttggc tactatctaa gatatgcgct
      781 tagttatcgt gatatatctg aaatactaag agaacgtggt gtcaacgttc atcattcaac
      841 ggtctaccgt tgggttcaag aatatgctcc cgttttgtat caaatttgga agaaaaaaca
      901 taaaaaagcg tattataagt ggcgtgttga tgagacatat atcaaaatta aaggacagtg
      961 gtgttatctg tatcgcgcga ttgatgcaga tggacataca ttagatattt ggttacgcaa
     1021 aaaacgagat catcaatcag catatgcgtt tattaagcgc cttattaaac aattcggtaa
     1081 acctcaaatg ataattacag atcaagcacc ttcaacgaag gtagccatgg ctaaaataat
     1141 taaagtgttt aagttgaaac ctaactgtca ttgcacatct aaatatctca ataacttcat
     1201 tgaacaagat catcgtcaca ttaaggtgag aaagacaaga tatcaaagtg tcaatacggc
     1261 aaaaaataca ctcaaaggca ttgaatgtat ttacggatta tataaaaaaa ccgtaggtct
     1321 cttcagatct acggattttc accatgccac gaaattagtt atatgttagc tggttaggcg
     1381 aagactgaca caataaaatg gtgattagct atatttttct
//
LOCUS       DAKTIO010000314         1408 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.325, whole genome shotgun sequence.
ACCESSION   DAKTIO010000314 DAKTIO010000000
VERSION     DAKTIO010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.325"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            629..1336
                     /gene="splA"
                     /locus_tag="O0293_002844"
     CDS             629..1336
                     /gene="splA"
                     /locus_tag="O0293_002844"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplA"
                     /protein_id="HCY0281653.1"
                     /translation="MNKNVMVKGLTALTILTSLGFAENISNQPHSIAKAEKNVKEITD
                     ATKEPYNSVVAFVGGTGVVVGKNTIVTNKHIAKSNDIFKNRVSAHHSSKGKGGGNYDV
                     KDIVEYPGKEDLAIVHVHETSTEGLNFNKNVSYTKFADGAKVKDRISVIGYPKGAQTK
                     YKMFESTGTINHISGTFMEFDAYAQPGNSGSPVLNSKHELIGILYAGSGKDESEKNFG
                     VYFTPQLKEFIQNNIEK"
BASE COUNT          569 a          190 c          211 g          438 t
ORIGIN      
        1 tcagaacaaa aaagacatgc atcaacatgt cagtgaaact aatccatatt atcttaacaa
       61 cgaaattgaa tcgaacctga aaaacgtatt tacaaatcgc aactactcta tccaaggcaa
      121 aatcgcaaaa tctgatcccc gaaataacat atctcgaaaa aaattccata attcaatcat
      181 cttcaatatt taacaataca ttactagttc caaatatact catacataca tgtttcattg
      241 attgactgca tattgtcata aataataggg gaactaatcg ctaataatgt tgttaaacac
      301 aatacatagt aaagtgaaag attataaata catgaattgt tcaccattcc gccatatatt
      361 ttcaatcacc taatttccga ataaatatta caattttaat tcaataatta atatttaaga
      421 ccaaaataat cctaaaaagt caattttaat tgcgtgatat tgagttttaa gcatatttaa
      481 ataaatgagt caattcatgt aaaataattt aataaaacgt taataagtgg ttaattaata
      541 tttaattaat atttttattt aaaatgtata tagtaaatgt gtatctagat acattaacta
      601 taaaaataaa tttggaagga ggtaaaatat gaataaaaat gtaatggtta aaggtttaac
      661 tgctttaact attttaacat ctcttggttt tgctgaaaat atttctaatc agcctcattc
      721 aattgccaaa gcagaaaaga atgtcaaaga aattaccgat gcaactaagg aaccatacaa
      781 ttcagtggta gcatttgtgg gtggtactgg tgtagttgtt ggtaaaaata caatcgtaac
      841 taacaaacat atcgctaaaa gtaatgatat ttttaaaaat agagtatcag cacatcattc
      901 gagtaaaggt aaaggcggag gaaactacga cgttaaagac attgtagaat atcccggaaa
      961 agaagacctt gcgatagttc atgttcatga aacaagtaca gaaggtttga attttaataa
     1021 gaacgttagt tatacaaaat ttgcagacgg agcaaaagtg aaagatagaa tttctgttat
     1081 tggttatcca aagggtgcac aaacaaaata taaaatgttt gaatcgacag gaacgattaa
     1141 ccatatcagt ggaacgttta tggaatttga tgcgtatgca caaccaggta attcaggatc
     1201 tcctgtattg aattctaaac atgaactgat tggtatttta tatgcaggta gtggaaaaga
     1261 tgaatctgaa aagaatttcg gtgtttattt cacaccacaa ttaaaagaat ttattcaaaa
     1321 taatattgaa aaataagtat catcaattca ttcgtgaagt tgatttttta aagagtgatt
     1381 aagaaaacgg ttacataatt aactaaat
//
LOCUS       DAKTIO010000315         1385 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.326, whole genome shotgun sequence.
ACCESSION   DAKTIO010000315 DAKTIO010000000
VERSION     DAKTIO010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.326"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            107..292
                     /locus_tag="O0293_002845"
     CDS             107..292
                     /locus_tag="O0293_002845"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HCY0281654.1"
                     /translation="MPIVNVKLLEGRSDEQLKNLVSEVTDAVEKTTGANRQAIHVVIE
                     EMKPNHYGVAGVRKSDQ"
     gene            complement(380..1363)
                     /locus_tag="O0293_002846"
     CDS             complement(380..1363)
                     /locus_tag="O0293_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCY0281655.1"
                     /translation="MDKETNDNEYRRQSEHRTSAPKRKKKKKIRKLPIILLIVVILLI
                     ALVVYIVHSYNSGVEYAKKHAKDVKVHQFNGPVKNDGKISILVLGADKAQGGQSRTDS
                     IMVVQYDFINKKMKMMSVMRDIYADIPGYGKHKINSAYALGGPELLRKTLDKNLGINP
                     EYYAVVDFTGFEKMIDELMPEGVPINVEKDMSKNIGVSLKKGNHRLNGKELLGYARFR
                     HDPEGDFGRVRRQQQVMQTLKKEMVNFRTVVKLPKVAGILRGYVNTNIPDSGIFQTGL
                     SFGIRGEKDVKSLTVPIKNSYEDVNTNTDGSALQINKNTNKQAIKDFLDED"
BASE COUNT          428 a          252 c          204 g          501 t
ORIGIN      
        1 gtgttctatt tcaactattt tagaacaaat gttctgttat tttgcaaatg aatttttaag
       61 tattacatga tatactcaaa ttaataatga ttaggaggtt tggatgatgc caatcgtcaa
      121 tgtaaaatta ttagaaggtc gttcggatga acaattaaaa aatttagtta gcgaagtaac
      181 tgacgccgta gaaaaaacaa cgggggcaaa tagacaagca attcacgttg ttatagaaga
      241 aatgaaacca aaccattatg gtgtggctgg cgtaagaaag tcagatcaat aattcttcat
      301 aagatgcatg ccaattaatt ctttgaaaac gaacaaggcg acttctatct gagtatgata
      361 gaaatcgcct tgtttatttt taatcttcat ctaaaaagtc tttaatagct tgtttatttg
      421 tgtttttatt aatctgtaat gcactaccat cagtatttgt attgacatct tcgtatgagt
      481 tcttgattgg cacagtcaat gacttaacat ctttttcacc tcggatacca aaactcaaac
      541 ctgtttggaa aatccctgaa tcaggaatgt ttgtattcac atagcctctt aaaatacctg
      601 caacttttgg taatttaaca actgttctaa aattaaccat ttcttttttc aatgtttgca
      661 tcacttgttg ctgacgtcgc acgcgtccga agtcaccttc agggtcgtga cggaatcttg
      721 cataaccaag taattcttta ccattcaacc tatggttacc ctttttcaaa gatacaccaa
      781 tatttttcga catatctttt tcgacattaa ttggtacacc ttcaggcatt aattcatcaa
      841 tcattttctc aaatccagta aaatcaacta ctgcataata ttcaggatta attcctaaat
      901 ttttatcaag tgtttttcta agtagctctg gaccacctaa agcgtatgct gaattaattt
      961 tgtgttttcc atatcctgga atatctgcat aaatatcacg catgacagac atcattttca
     1021 tctttttatt gataaagtca tattgaacaa ccatgataga atctgttctt gattgtccac
     1081 cttgtgcttt atctgcaccg agtacaagaa tagaaatttt accatcattt tttactggtc
     1141 cattaaattg atgtacttta acatctttcg catgtttctt ggcatattct acaccgctat
     1201 tgtaactatg tacaatatat acaactaatg cgataagtaa aattacaaca atcagaagaa
     1261 tgataggtaa tttcctaatt ttcttcttct tttttcgctt aggcgccgaa gtgcgatgtt
     1321 cactttgacg tctatattcg ttgtcattag tttctttatc catatttacc taccttatat
     1381 cttca
//
LOCUS       DAKTIO010000316         1366 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.327, whole genome shotgun sequence.
ACCESSION   DAKTIO010000316 DAKTIO010000000
VERSION     DAKTIO010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.327"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..174
                     /locus_tag="O0293_002847"
     CDS             <1..174
                     /locus_tag="O0293_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HCY0281656.1"
                     /translation="KRYDIPTSKAPKLLLKGSGNLKGSSVGYKDIEFTFVEKKEENIY
                     FSDSLDYKKSGDV"
     gene            226..996
                     /locus_tag="O0293_002848"
     CDS             226..996
                     /locus_tag="O0293_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0281657.1"
                     /translation="MIKRVNKLVLGISLLFLVISITAGCGMGKEAEIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIVNSQMAIQNKGEALKIKGMLLKIDRNTRSA
                     KGFYYTNEIKTEKYEVAQDNQKKYPVKMINNKFISTEEVKEENIKKEIENFKFFAQYS
                     NFKDLMNYKDGDISYNPEVPSYSAQYQLTNDDYNVKQLRKRYDIPTNKAPKLLLKGTG
                     NLKGSSVGYKKIEFTFLENKNENIYFTDSLHLEPSEDK"
     gene            1060..>1366
                     /locus_tag="O0293_002849"
     CDS             1060..>1366
                     /locus_tag="O0293_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HCY0281658.1"
                     /translation="MMKRLNKLVLGIIFLFLVISITAGCGIGKEAKIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDEFDKNDKGTWIIGSEMATQNKGEALKVKGMVLYMNRNTKTT
                     "
BASE COUNT          578 a          138 c          263 g          387 t
ORIGIN      
        1 aaaagatatg atataccgac gagtaaagct ccaaagttat tgttaaaagg ttcagggaat
       61 ttaaaaggct catcagttgg atataaagat attgaattta cgtttgtaga gaaaaaagaa
      121 gaaaatatat actttagtga tagcttagat tataaaaaaa gcggagatgt ataatcatgg
      181 ctcaatcaga atatgaaatc aatcccggaa aaagagagtg atgaaatgat aaaacgtgta
      241 aataaattag tgcttggtat tagtcttctg tttttagtca ttagtatcac tgctggttgt
      301 ggcatgggta aagaagcgga aataaagaaa agttttgaaa aaacattgag tatgtatccg
      361 attaaaaatc tagaggattt atacgataaa gaaggatatc gtgatgatca atttgataaa
      421 aatgataagg gaacatggat tgtaaattct caaatggcaa ttcaaaataa aggagaagct
      481 ctaaaaataa aaggcatgct tttgaagata gatagaaata caagaagtgc aaaaggattt
      541 tactatacta atgaaataaa gacggagaaa tacgaagtag ctcaggataa tcaaaaaaaa
      601 tatccagtta aaatgattaa taataaattc atttctactg aggaagttaa agaagaaaac
      661 ataaaaaaag aaatcgaaaa ctttaagttt tttgcgcaat atagcaattt taaagattta
      721 atgaattata aagatggaga tatatcatat aatccagagg tgccgagtta ttcagctcaa
      781 tatcaattaa ctaatgatga ttataatgta aaacaattac gtaaaagata tgacatacca
      841 acaaataaag cgccgaagct gttgttgaaa ggtacaggga atttaaaagg ttcatcagtt
      901 ggatataaaa aaattgaatt tactttttta gagaataaaa atgaaaatat ttactttact
      961 gatagtctac atcttgaacc gagcgaggat aaataatcat cttttaacca gaatatgaaa
     1021 tcaatctagg acaacctaat aaaaggagag agttgtaaga tgatgaaacg attaaacaaa
     1081 ttagtgttag gcattatttt tctgttttta gtcattagta tcactgctgg ttgtggcata
     1141 ggtaaagaag cgaaaataaa gaaaagtttt gagaagacat tgagtatgta tccgattaaa
     1201 aatctagagg atttatacga taaagaaggt tatcgtgatg acgaatttga taaaaatgat
     1261 aaaggtacat ggattattgg ttctgaaatg gcaactcaaa ataaggggga agctctgaaa
     1321 gttaaaggta tggtcttgta tatgaataga aataccaaaa caacaa
//
LOCUS       DAKTIO010000317         1355 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.329, whole genome shotgun sequence.
ACCESSION   DAKTIO010000317 DAKTIO010000000
VERSION     DAKTIO010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.329"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..1318
                     /locus_tag="O0293_002850"
     rRNA            <1..1318
                     /locus_tag="O0293_002850"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          358 a          302 c          399 g          296 t
ORIGIN      
        1 cactgccgag aaaagcctct agatagaaaa taggtgcccg taccgcaaac cgacacaggt
       61 agtcaagatg agaattctaa ggtgagcgag cgaactctcg ttaaggaact cggcaaaatg
      121 accccgtaac ttcgggagaa ggggtgctct ttagggttaa cgcccagaag agccgcagtg
      181 aataggccca agcgactgtt tatcaaaaac acaggtctct gctaaaccgt aaggtgatgt
      241 ataggggctg acgcctgccc ggtgctggaa ggttaagagg agtggttagc ttctgcgaag
      301 ctacgaatcg aagccccagt aaacggcggc cgtaactata acggtcctaa ggtagcgaaa
      361 ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa cgatttgggc actgtctcaa
      421 cgagagactc ggtgaaatca tagtacctgt gaagatgcag gttacccgcg acaggacgga
      481 aagaccccgt ggagctttac tgtagcctga tattgaaatt cggcacagct tgtacaggat
      541 aggtaggagc ctttgaaacg tgagcgctag cttacgtgga ggcgctggtg ggatactacc
      601 ctagctgtgt tggctttcta acccgcacca cttatcgtgg tgggagacag tgtcaggcgg
      661 gcagtttgac tggggcggtc gcctcctaaa aggtaacgga ggcgctcaaa ggttccctca
      721 gaatggttgg aaatcattca tagagtgtaa aggcataagg gagcttgact gcgagaccta
      781 caagtcgagc agggtcgaaa gacggactta gtgatccggt ggttccgcat ggaagggcca
      841 tcgctcaacg gataaaagct accccgggga taacaggctt atctccccca agagttcaca
      901 tcgacgggga ggtttggcac ctcgatgtcg gctcatcgca tcctggggct gtagtcggtc
      961 ccaagggttg ggctgttcgc ccattaaagc ggtacgcgag ctgggttcag aacgtcgtga
     1021 gacagttcgg tccctatccg tcgtgggcgt aggaaatttg agaggagctg tccttagtac
     1081 gagaggaccg ggatggacat acctctggtg taccagttgt cgtgccaacg gcatagctgg
     1141 gtagctatgt gtggacggga taagtgctga aagcatctaa gcatgaagcc cccctcaaga
     1201 tgagatttcc caacttcggt tataagatcc ctcaaagatg atgaggttaa taggttcgag
     1261 gtggaagcat ggtgacatgt ggagctgacg aatactaatc gatcgaagac ttaatcaaaa
     1321 taaatgtttt gcgaagcaaa atcactttta cttac
//
LOCUS       DAKTIO010000318         1349 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.330, whole genome shotgun sequence.
ACCESSION   DAKTIO010000318 DAKTIO010000000
VERSION     DAKTIO010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.330"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(98..>1349)
                     /locus_tag="O0293_002851"
     CDS             complement(98..>1349)
                     /locus_tag="O0293_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCY0281659.1"
                     /translation="HLLEDRDVLCIDQKSFFASVSCIEKGLDPLETKLAVVADTKRQG
                     SVILAATPKLKELGIKTGSRLFEIPHRNDIYIINPSMRKYLNVSVAISKIALRYIPPE
                     DLHQYSIDEFFMDVTDSYHRFSSTVHAFCERLKREIYEETGIYCTVGIGSNMLLSKIA
                     MDVEAKHSQNGIAEWRYQDVPTKLWPIQPLRDFWGINRRTEAKLNKRGIFTIGDLAKY
                     PYKFLKKEFGILGVDMHLHANGIDQSKVREKHKISNPSICKSQILMRDYHFDEAKVVM
                     QELIEDVASRVRARKKVARTIHFAFGYSDEGGVHKQYTLKDPTNLEKDIYKVVMHFAD
                     KLCNKQALYRTLSISLSQFINEDERQLSLFEDEYQRKRDECLAKTIDQLHLKYGKGIV
                     SKAVSFTEAGTKHGRLGLMAGHKM"
BASE COUNT          411 a          268 c          178 g          492 t
ORIGIN      
        1 tattattgtg aaaatctatt gtgatatatg aaatatttat ctttagagac ttattttata
       61 cgaatcacag ttatatatta cttaaaccgt atagtcatta cattttatgt ccagccatta
      121 aacctaatct gccgtgtttt gtacctgctt ctgtaaacga tactgctttg gacacaatac
      181 ctttgccgta tttcaaatgt aattggtcta tcgttttagc tagacattcg tcacgtttgc
      241 gttggtattc atcttcaaac agacttaact gtcgctcatc ctcattaata aattgactca
      301 aagatatact tagcgtacga tatagtgctt gtttattaca taatttatct gcgaaatgca
      361 ttactacttt ataaatatct ttttctaagt ttgttggatc tttcaaagta tattgcttat
      421 gtacaccgcc ttcatcacta tagccaaagg caaaatgtat cgttcttgcc actttttttc
      481 ttgctcgaac tctgctagca acatcttcaa ttaactcttg cattactact tttgcttcat
      541 caaaatgata atctctcatt aatatttgac ttttgcatat cgatggattg ctgatcttgt
      601 gcttttcacg tactttactc tgatctatcc cattcgcatg tagatgcata tcaacaccta
      661 aaataccgaa ctcttttttt aaaaatttat atggatattt cgctaaatct cctatagtaa
      721 aaattcctct tttattcaat ttggcttctg ttcgacgatt aataccccaa aaatctcgca
      781 agggctgaat tggccataat ttcgttggta catcttgata tcgccattca gctataccat
      841 tttgactatg cttcgcttca acatccatag caattttact taataacata ttagaaccaa
      901 tgcccacagt acaataaatg cctgtttctt cataaatttc acgtttaagt ctttcgcaaa
      961 atgcatgtac tgtagaacta aatctatgat agctatcagt aacatccata aaaaattcgt
     1021 caatactata ttggtgtaaa tcttcaggtg gaatataacg caatgcaatc ttagaaatag
     1081 caactgaaac attaagatat ttacgcatac ttggattgat aatgtaaata tcatttctat
     1141 gtggtatttc aaacaatcgc gaccctgtct tgatgcctaa ttcttttaat ttaggtgtcg
     1201 cagccaatat tacagaaccc tgacgcttag tatctgcaac aacagctagc tttgtttcta
     1261 atggatctag ccccttttca atacaagaaa cactcgcaaa aaaacttttt tggtcaatac
     1321 atagaacatc cctatcttct aataaatga
//
LOCUS       DAKTIO010000319         1345 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.331, whole genome shotgun sequence.
ACCESSION   DAKTIO010000319 DAKTIO010000000
VERSION     DAKTIO010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.331"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(204..557)
                     /locus_tag="O0293_002852"
     CDS             complement(204..557)
                     /locus_tag="O0293_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HCY0281660.1"
                     /translation="MISIILVMIGGGFGAIARSAITDYFNHKFTSKLPIATLIVNLVG
                     SFLIGLTIGLSISISWFPAFFVTGFLGGLTTFSTLAKELTLMMTPKFNINLFLNYSLL
                     QFIIGFIACYIGYHI"
     gene            complement(554..919)
                     /gene="crcB"
                     /locus_tag="O0293_002853"
     CDS             complement(554..919)
                     /gene="crcB"
                     /locus_tag="O0293_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCY0281661.1"
                     /translation="MQYVYIFIGGALGALLRYLISFLNTDGGFPIGTLIANLTGAFVM
                     GLLTALTIAFFSNHPTLKKAITTGFLGALTTFSTFQLELIHMFDHQQFITLLLYAVTS
                     YVFGILLCYVGIKLGGDLS"
     gene            complement(1175..1324)
                     /locus_tag="O0293_002854"
     CDS             complement(1175..1324)
                     /locus_tag="O0293_002854"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281662.1"
                     /translation="MSIIFADRSIKSLKPDADWIRIASKSLPWIILIVILVHIFSIVR
                     TFGFI"
BASE COUNT          506 a          197 c          216 g          426 t
ORIGIN      
        1 ttttttaggg atttatgtcc cactcccttt attttcgact agtttggaga atttattgac
       61 attcacatta tttaaacggc aacaaagatt attgaaattt ggtaggcatt atatggtgct
      121 taaaaatttg catgaaaatt aaaaaatgct tcgttcagga aggtgtcgta atttacctat
      181 ttgctgaatg aagcatttta tttttaaata tgatagccaa tataacaagc tataaatcca
      241 atgatgaatt gtaaaagtga ataattgaga aaaaggttaa tattaaattt tggcgtcatc
      301 attagtgtaa gttctttggc taacgttgag aaagttgtta agccacctaa aaaaccggta
      361 acaaagaacg cagggaacca tgagattgaa attgataagc ctatagttaa tccaattaaa
      421 aaactaccaa ctagatttac tatcaatgtt gcgataggta actttgaagt aaatttatga
      481 ttaaaataat cagtaatggc acttctagca attgcgccaa aaccgccgcc aatcatgact
      541 aaaatgattg atatcatgat aaatcaccac ctagttttat accgacgtaa cataacaaaa
      601 taccaaagac ataacttgtt acagcatata gtagtaaagt tataaattgt tgatgatcaa
      661 acatatgtat taattctaat tgaaatgttg aaaaagtcgt taaagcacca agaaaaccag
      721 tcgtaatagc tttttttagg gtcggatggt ttgaaaaaaa tgcaattgtt aaggctgtta
      781 gcaatcccat tacaaaggca ccagtcaaat tggctatcag tgttccgatt ggaaaacctc
      841 cgtcagtatt cagaaaagaa atgaggtaac gtaataaagc gcctaaagca ccaccgataa
      901 aaatatatac atattgcatt tggttcacct cgaaaagaag tagtttgaat ttaaaaaaga
      961 ggttttggca acgcgacgac aaaaattgtc gatgcattat caaacctcat tatatgttat
     1021 atcttgttgt ataactatag cgattagatg catagttatg atttcgaaaa tctaatattt
     1081 tttatacgca acaacgttat caaattgttt tactcattat agcatgatac attgtattgt
     1141 tttgtattaa ggctacaatg acatattaac ttttttaaat aaaaccgaat gtacgaacaa
     1201 ttgaaaagat atgtactaaa ataacaatta gaataatcca aggcaaactt ttactcgcaa
     1261 ttctaatcca atctgcatca ggctttagtg atttaattga acgatctgca aaaattatag
     1321 acaaaattag tacaattgag ttaat
//
LOCUS       DAKTIO010000320         1312 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.332, whole genome shotgun sequence.
ACCESSION   DAKTIO010000320 DAKTIO010000000
VERSION     DAKTIO010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.332"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(110..958)
                     /locus_tag="O0293_002855"
     CDS             complement(110..958)
                     /locus_tag="O0293_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCY0281663.1"
                     /translation="MNHIEISKDVKIPVLGFGVFQIPQEQTAEAVKEAIKAGYRHIDT
                     AQSYLNETEVGQGIEASGIDRSELFITTKVWIENVNYEDTIKSIERSLQRLNLDYLDL
                     VLIHQPYNDVYGSWRALEELKENGKIKAIGVSNFGVDRIVDLGIHNQIQPQVNQIEIN
                     PFHQQEEQVAALQQENVVVEAWAPFAEGKNQLFQNQLLQAIADKYNKSIAQVILRWLV
                     ERDIVVLAKSVNPERMAQNLDIFDFELTEEDKQQIATLEESNSQFFSHADPEMIKALT
                     SRELDV"
     gene            complement(1028..>1312)
                     /locus_tag="O0293_002856"
     CDS             complement(1028..>1312)
                     /locus_tag="O0293_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCY0281664.1"
                     /translation="NWIYLVKNLRNAGVSIESLIEFCRLAQLPKNENIQAQQKQILNK
                     QLEELNENLKTIHDVRDLLQYKIDNYDNHIAKINASDNYDDNVERLWERK"
BASE COUNT          378 a          237 c          187 g          510 t
ORIGIN      
        1 ttgcttgctc ctttcacttt ttaagagtaa aaaataacct gaaacataga ctttaacatc
       61 caagtttcag gttagtgtaa tgtaaatgat ttactttgtt atattatgat taaacatcta
      121 attctctgct tgtaagtgct ttaatcatct ctggatcagc atgtgagaaa aattggctat
      181 tactttcttc taatgtcgca atttgttgtt tatcttcttc tgtaagttca aaatcaaata
      241 tatctaagtt ttgagccata cgttctggat tcactgattt tgcgagtaca acaatgtcac
      301 gttctactaa ccagcgtaaa ataacttgtg caattgattt gttgtattta tctgcaatgg
      361 cctgcaacaa ttgattttgg aataattgat ttttaccttc tgcaaatggt gcccaagctt
      421 ctaccacaac attttcttgt tgaagtgctg caacttgttc ttcttgttga tggaatggat
      481 taatttctat ttggttcact tgtggttgaa tttgattgtg aatacctaaa tctacaattc
      541 gatcaacgcc gaagttagat acaccgattg ctttaatttt accattttct tttaactctt
      601 ctaatgcacg ccatgagcca tatacgtcat tgtaaggttg gtgaataagc actagatcaa
      661 gatagtccaa atttaatcgt tgtaatgatc gttcgattga tttgatagta tcttcatagt
      721 taacattttc aatccatact ttagtcgtaa tgaatagttc acttctatca atacctgaag
      781 cttcaatacc ttgacctact tcagtttcat ttaaataact ttgtgctgta tcaatatgtc
      841 tataacccgc tttaattgct tctttaaccg cttctgctgt ttgttcttga ggaatttgaa
      901 atactccgaa acctaatact ggtatcttta catctttact tatttcaata tgattcataa
      961 aatgaacctc ctttatcttg tatgtccact ataaaccatg gagcgaactc tatagcaagc
     1021 cataggttta ctttctctcc caaagacgtt caacattgtc atcataatta tcactagcat
     1081 tgattttagc aatatgatta tcataattat caattttata ttgtagtaaa tctctcacat
     1141 catgaattgt ctttaagttt tcatttaatt cttcgagttg cttatttaaa atttgctttt
     1201 gctgtgcttg aatattttca tttttaggaa gctgcgctaa cctgcaaaat tcgataagcg
     1261 attcaatact gacgcctgca tttcgcaaat ttttcactaa ataaatccaa tt
//
LOCUS       DAKTIO010000321         1308 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.333, whole genome shotgun sequence.
ACCESSION   DAKTIO010000321 DAKTIO010000000
VERSION     DAKTIO010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.333"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            230..>1308
                     /locus_tag="O0293_002857"
     CDS             230..>1308
                     /locus_tag="O0293_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0281665.1"
                     /translation="MAKSCLHILTNNEYATTRCQDGIVLFWPIDGEIELQKFRKSKII
                     EDDIYIINHLDVFSIKNNKKTIMLYLSSDWFAELGFTFFNYHYTAKLIKSSYNLKCLL
                     LKLTYRYLDNQPLNDADIRKLQDIIKIIAKEASMDKKIAQNQYRYAYYGDLRDELEYI
                     YQNVNQRLTLKSVADKLFVSKSNLSSQFHLLMGMGFKKYIDTLKIGKSIEILLTTDST
                     ISNISEHLGFSSSSTYSKMFKSYMDITPNEYRNLSKYNKCLMLKPEPLVGKMVQEVKE
                     IILNYIEHYKNHLTDVIHIDEDKFETPKLFQTVIQINTYTEMKLVFLEGIFKTLLNKS
                     SQVVFFIMPSILKSKNTMSEEEKFT"
BASE COUNT          512 a          155 c          196 g          445 t
ORIGIN      
        1 aaaatactaa aaagtagtgt gttttcaatt ggatagttta attaaactga ctacatctat
       61 ttttgtaata tttaaatgat tgatatataa gtttaaaaat taaccattat atagttttat
      121 ttatttttga tttggtattc gtattgtgtt aaatttacgt ttaattaaat aagttaaata
      181 ttatagggaa tgataaaagt ggtacttaaa tatagaaaga ggtaaagtta tggcaaaatc
      241 atgcttgcat atacttacta ataatgaata tgcgacaacg cgttgccaag atggcatagt
      301 cttattttgg ccaattgacg gggaaatcga actacaaaaa tttcgtaaaa gtaaaataat
      361 tgaagatgat atatatatta ttaatcatct ggatgtattt agtattaaga ataataaaaa
      421 aacgatcatg ttgtatttga gtagcgattg gtttgcggaa ttaggcttta ctttctttaa
      481 ttaccactat acagcaaagt tgattaaatc atcctataat ttgaaatgtc tactattaaa
      541 attgacatat cgataccttg ataatcagcc tcttaatgac gctgatatta gaaaattaca
      601 ggatattatt aaaatcattg caaaagaagc aagtatggat aaaaagattg cacaaaatca
      661 atatcgatat gcgtattatg gtgatttgcg tgatgagctc gaatatattt atcaaaatgt
      721 aaatcaacga ttgacattaa aaagtgtcgc tgataaatta tttgtctcaa agtcaaattt
      781 gtcatcacaa ttccacttac ttatgggcat gggttttaaa aaatatattg atactttgaa
      841 aattggtaaa tcgattgaaa ttctacttac tactgatagt actattagca acataagtga
      901 acatttaggt tttagtagta gctccactta ctctaaaatg tttaaaagtt atatggatat
      961 aacaccgaat gaatatcgta atttatcaaa atataataaa tgtttaatgc taaagccaga
     1021 accactagta ggcaaaatgg tgcaagaagt aaaagaaatc atattgaatt atattgaaca
     1081 ttataaaaac cacctaactg atgttataca tattgatgaa gacaaatttg aaacacctaa
     1141 attgtttcaa acggttattc aaataaatac ttatacagaa atgaaattag ttttcttaga
     1201 aggaatcttt aaaaccttat tgaataagag cagtcaagtt gtctttttca tcatgccatc
     1261 gattctaaaa agtaaaaata ccatgtccga agaagaaaaa ttcacaat
//
LOCUS       DAKTIO010000322         1298 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.334, whole genome shotgun sequence.
ACCESSION   DAKTIO010000322 DAKTIO010000000
VERSION     DAKTIO010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.334"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            72..842
                     /gene="fabI"
                     /locus_tag="O0293_002858"
     CDS             72..842
                     /gene="fabI"
                     /locus_tag="O0293_002858"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCY0281666.1"
                     /translation="MLNLENKTYVIMGIANKRSIAFGVAKVLDQLGAKLVFTYRKERS
                     RKELEKLLEQLNQPEAHLYQIDVQSDEEVINGFEQIGKDVGNIDGVYHSIAFANMEDL
                     RGRFSETSREGFLLAQDISSYSLTIVAHEAKKLMPEGGSIVATTYLGGEFAVQNYNVM
                     GVAKASLEANVKYLALDLGPDNIRVNAISAGPIRTLSAKGVGGFNTILKEIEERAPLK
                     RNVDQVEVGKTAAYLLSDLSSGVTGENIHVDSGFHAIK"
     gene            complement(1037..>1298)
                     /locus_tag="O0293_002859"
     CDS             complement(1037..>1298)
                     /locus_tag="O0293_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCY0281667.1"
                     /translation="TFIWVCIITLIAHQLEGNIITPNVMGKSLSIHPLTIIVVILAAG
                     DLGGFTLVLIAVPLYAVLKTVVSNIFKYRQRIIDKANSNVKD"
BASE COUNT          447 a          189 c          237 g          425 t
ORIGIN      
        1 ataaaaagtt agttaaaact gggattagat attctatccg ttaaattaat tattataagg
       61 agttatctta catgttaaat cttgaaaaca aaacatatgt catcatggga atcgctaata
      121 agcgtagtat tgcttttggt gtcgctaaag ttttagatca attaggtgct aaattagtat
      181 ttacttaccg taaagaacgt agccgtaaag agcttgaaaa attattagaa caattaaatc
      241 aaccagaagc gcacttatat caaattgatg ttcaaagcga tgaagaggtt attaatggtt
      301 ttgagcaaat tggtaaagat gttggcaata ttgatggtgt atatcattca atcgcatttg
      361 ctaatatgga agacttacgc ggacgctttt ctgaaacttc acgtgaaggc ttcttgttag
      421 ctcaagacat tagttcttac tcattaacaa ttgtggctca tgaagctaaa aaattaatgc
      481 cagaaggtgg tagcattgtt gcaacaacat atttaggtgg cgaattcgca gttcaaaact
      541 ataatgtgat gggtgttgct aaagcgagct tagaagcaaa tgttaaatat ttagcattag
      601 acttaggtcc agataatatt cgcgttaatg caatttcagc tggtccaatc cgtacattaa
      661 gtgcaaaagg tgtgggtggt ttcaatacaa ttcttaaaga aatcgaagag cgtgcacctt
      721 taaaacgtaa tgttgatcaa gtagaagtag gtaaaactgc ggcttactta ttaagtgatt
      781 tatcaagtgg cgttacaggt gaaaatattc atgtagatag cggattccac gcaattaaat
      841 aatatcattc aacagctttg ttcacgttat tatatatgtg agcaaagctt ttttgctttt
      901 ataataatcg gactgatgga aaattatttg atatttcatt tgactgattt tttatgcaca
      961 aaaaaatcgc ctcaaaagct gtagtttcaa cttttgatgc gatagtatct tatattctta
     1021 aaagacatcc acagaattag tccttaacat tactgtttgc tttatcaata atgcgttggc
     1081 ggtatttgaa aatattacta acaaccgttt taagtacagc atataatggc actgcaatca
     1141 gaactaatgt aaagccacct aaatctcctg ctgctaaaat aacaacgata attgttaaag
     1201 gatggatact taaagattta cccattacat ttggtgtaat gatattacct tcaagttgat
     1261 gtgcaattaa tgtaatgata caaacccata taaatgta
//
LOCUS       DAKTIO010000323         1293 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.335, whole genome shotgun sequence.
ACCESSION   DAKTIO010000323 DAKTIO010000000
VERSION     DAKTIO010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.335"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            266..1225
                     /gene="hyl"
                     /locus_tag="O0293_002860"
     CDS             266..1225
                     /gene="hyl"
                     /locus_tag="O0293_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hemolysin"
                     /protein_id="HCY0281668.1"
                     /translation="MKTRIVSSVTTTLLLGSILMNPVANAADSDINIKTGTTDIGSNT
                     TVKTGDLVTYDKENGMHKKVFYSFIDDKNHNKKLLVIRTKGTIAGQYRVYSEEGANKS
                     GLAWPSAFKVQLQLPDNEVAQISDYYPRNSIDTKEYMSTLTYGFNGNVTGDDTGKIGG
                     LIGANVSIGHTLKYVQPDFKTILESPTDKKVGWKVIFNNMVNQNWGPYDRDSWNPVYG
                     NQLFMKTRNGSMKAADNFLDPNKASSLLSSGFSPDFATVITMDRKASKQQTNIDVIYE
                     RVRDDYQLHWTSTNWKGTNTKDKWIDRSSERYKIDWEKEEMTN"
BASE COUNT          495 a          196 c          209 g          393 t
ORIGIN      
        1 atgtcttttc cttgtttcat aaagaattta ttcaactttg actaaccctc gaaattgaaa
       61 tgcttccttt caaattttaa ataaaagtta aaaacatatt cttaaaataa tcactcatca
      121 tcactcagta atttatcagt tgctacatta aatatactaa tataaacgat tataaatatt
      181 tgatatgtct caactgcaat attctaaatt gacatattga ttattgtttc ctcaaatcgt
      241 tttaaaaaat agaaggatga tgaaaatgaa aacacgtata gtcagctcag taacaacaac
      301 actattgcta ggttccatat taatgaatcc tgtcgctaat gccgcagatt ctgatattaa
      361 tattaaaacc ggtactacag atattggaag caatactaca gtaaaaacag gtgatttagt
      421 cacttatgat aaagaaaatg gcatgcacaa aaaagtattt tatagtttta tcgatgataa
      481 aaatcataat aaaaaactgc tagttattag aacgaaaggt accattgctg gtcaatatag
      541 agtttatagc gaagaaggtg ctaacaaaag tggtttagcc tggccttcag cctttaaggt
      601 acagttgcaa ctacctgata atgaagtagc tcaaatatct gattactatc caagaaattc
      661 gattgataca aaagagtata tgagtacttt aacttatgga ttcaacggta atgttactgg
      721 tgatgataca ggaaaaattg gcggccttat tggtgcaaat gtttcgattg gtcatacact
      781 gaaatatgtt caacctgatt tcaaaacaat tttagagagc ccaactgata aaaaagtagg
      841 ctggaaagtg atatttaaca atatggtgaa tcaaaattgg ggaccatatg atagagattc
      901 ttggaacccg gtatatggca atcaactttt catgaaaact agaaatggct ctatgaaagc
      961 agcagataac ttccttgatc ctaacaaagc aagttctcta ttatcttcag ggttttcacc
     1021 agacttcgct acagttatta ctatggatag aaaagcatcc aaacaacaaa caaatataga
     1081 tgtaatatac gaacgagttc gtgatgacta ccaattgcac tggacttcaa caaattggaa
     1141 aggtaccaat actaaagata aatggataga tcgttcttca gaaagatata aaatcgattg
     1201 ggaaaaagaa gaaatgacaa attaatgtaa attatttgtt catgtacaaa taaatataat
     1261 ttataacttt agccgataac ttcagaaatg atg
//
LOCUS       DAKTIO010000324         1277 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.336, whole genome shotgun sequence.
ACCESSION   DAKTIO010000324 DAKTIO010000000
VERSION     DAKTIO010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.336"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            35..133
                     /locus_tag="O0293_002861"
     CDS             35..133
                     /locus_tag="O0293_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCY0281669.1"
                     /translation="MIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     gene            293..1063
                     /gene="aadD1"
                     /locus_tag="O0293_002862"
     CDS             293..1063
                     /gene="aadD1"
                     /locus_tag="O0293_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-nucleotidyltransferase
                     ANT(4')-Ia"
                     /protein_id="HCY0281670.1"
                     /translation="MRIVNGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLG
                     RQTDGPYSDIEMMCVMSTEEAEFSHEWTTGEWKVEVNFDSEEILLDYASQVESDWPLT
                     HGQFFSILPIYDSGGYLEKVYQTAKSVEAQTFHDAICALIVEELFEYAGKWRNIRVQG
                     PTTFLPSLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQSDLPSGYDHLCQFVMSG
                     QLSDSEKLLESLENFWNGIQEWTERHGYIVDVSKRIPF"
BASE COUNT          414 a          184 c          302 g          377 t
ORIGIN      
        1 aaaattaaac cttgatgaca cagaagaagg cgatttgatt catacagatg atgacgaaaa
       61 agccgatgaa gatggatttt ctattattgc aatgtggaat tgggaacgga aaaattattt
      121 tattaaagag tagttcaaca aacgggccag tttgttgaag attagatgct ataattgtta
      181 ttaaaaggat tgaaggatgc ttaggaagac gagttattaa tagctgaata agaacggtgc
      241 tctccaaata ttcttattta gaaaagcaaa tctaaaatta tctgaaaagg gaatgagaat
      301 agtgaatgga ccaataataa tgactagaga agaaagaatg aagattgttc atgaaattaa
      361 ggaacgaata ttggataaat atggggatga tgttaaggct attggtgttt atggctctct
      421 tggtcgtcag actgatgggc cctattcgga tattgagatg atgtgtgtca tgtcaacaga
      481 ggaagcagag ttcagccatg aatggacaac cggtgagtgg aaggtggaag tgaattttga
      541 tagcgaagag attctactag attatgcatc tcaggtggaa tcagattggc cgcttacaca
      601 tggtcaattt ttctctattt tgccgattta tgattcaggt ggatacttag agaaagtgta
      661 tcaaactgct aaatcggtag aagcccaaac gttccacgat gcgatttgtg cccttatcgt
      721 agaagagctg tttgaatatg caggcaaatg gcgtaatatt cgtgtgcaag gaccgacaac
      781 atttctacca tccttgactg tacaggtagc aatggcaggt gccatgttga ttggtctgca
      841 tcatcgcatc tgttatacga cgagcgcttc ggtcttaact gaagcagtta agcaatcaga
      901 tcttccttca ggttatgacc atctgtgcca gttcgtaatg tctggtcaac tttccgactc
      961 tgagaaactt ctggaatcgc tagagaattt ctggaatggg attcaggagt ggacagaacg
     1021 acacggatat atagtggatg tgtcaaaacg cataccattt tgaacgatga cctctaataa
     1081 ttgttaatca tgttggttac gtatttatta acttctccta gtattagtaa ttatcatggc
     1141 tgtcatggcg cattaacgga ataaagggtg tgcttaaatc gggccatttt gcgtaataag
     1201 aaaaaggatt aattatgagc gaattgaatt aataataagg taatagattt acattagaaa
     1261 atgaaagggg attttat
//
LOCUS       DAKTIO010000325         1271 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.337, whole genome shotgun sequence.
ACCESSION   DAKTIO010000325 DAKTIO010000000
VERSION     DAKTIO010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.337"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            145..1113
                     /locus_tag="O0293_002863"
     CDS             145..1113
                     /locus_tag="O0293_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCY0281671.1"
                     /translation="MLLNLLLIFILIVICPLIFNYTLTLNLTCYILVVISIFLSLIYS
                     IMTYTFPWSMFVTFIIISIIMLFKHRYLFSHTSSRLFIKKLLSFSYKFVLYTSALLFI
                     STIQSPIVKGLSMWLAILCLSLLLTFICYLVWTVSYRHRSYNKEVDIIIVLGAGIFTE
                     FVTPMLAARLDRALDIYQQQASATKIIVSGGQGPDEPISEALAMQRYLIAHGVDKTDI
                     LMESHSINTYTNFLYSKQIINNLYHEAVKIVCVTSQFHVLRALRLAQKLGLSIDGVGS
                     HTPYHFFLHVLIKDYLGVMYQYRLLLTLYCSSSWFICLYAAFIYNS"
BASE COUNT          413 a          208 c          169 g          481 t
ORIGIN      
        1 gtatcgcaca tctaaaaaac acaaaaatat tgacccatat ataatataac aaattaagat
       61 ttacatgttg ctgctttctc cttgctttaa taaattaaat catctacatt tatatttaat
      121 atttattata agcgaggtgt cagtatgtta ttgaatttat tattaatatt tatacttatc
      181 gttatttgtc cgttaatttt caactatact ctgacgttaa atttaacatg ctacatctta
      241 gttgtcatat cgatattttt gtcactcatc tatagcatca tgacctatac tttcccttgg
      301 tctatgtttg taacatttat aataatcagt attatcatgc tattcaaaca tagatattta
      361 ttttctcata cttctagtcg cttatttatt aaaaaattat tatctttttc atataaattt
      421 gtactctaca catcagcact tctatttata agtacgattc agtcacctat tgtgaaaggt
      481 ttgtctatgt ggttagctat attatgtctt tccctgttac taacctttat ttgttatctc
      541 gtttggacag tatcatatag acatagatca tacaacaaag aagttgatat cataattgtg
      601 ttaggtgctg gtatttttac cgaatttgtc acaccaatgc ttgcagcgcg tttagatcgt
      661 gcacttgata tctatcaaca acaagcatca gcaacaaaga tcattgtatc tggcggacaa
      721 ggaccagacg aaccaatttc tgaagcttta gctatgcaac gttatttgat tgcacatggt
      781 gttgataaaa cagatatact aatggaatct cactctataa atacatacac aaacttttta
      841 tattctaaac aaatcatcaa caatttgtat catgaagcag ttaaaattgt ttgtgtaaca
      901 agtcagtttc atgtattgcg cgccttacga ctagcccaaa aattaggttt atctatcgat
      961 ggtgtcggaa gtcatacacc atatcacttt ttcttacatg tgctcatcaa agattatcta
     1021 ggcgtaatgt atcagtatag attgttatta acgttatatt gttcaagtag ttggtttata
     1081 tgtctttacg ccgcttttat ttataatagt taatccacaa acaaactatt attataggtt
     1141 gtttctaata ttgcgtcaaa ctatctttaa atattcgtta atccttgatt agacaattgt
     1201 tatattgaaa ataaataaac taaagagtct gggacattaa tccctaaaaa aaacagcagt
     1261 aagataattt t
//
LOCUS       DAKTIO010000326         1246 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.338, whole genome shotgun sequence.
ACCESSION   DAKTIO010000326 DAKTIO010000000
VERSION     DAKTIO010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.338"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..>1246
                     /locus_tag="O0293_002864"
     CDS             <1..>1246
                     /locus_tag="O0293_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HCY0281672.1"
                     /translation="IENVYKILNSDYYKKQDSDKLSKAAIDGMVKELKDPYSEYLTKE
                     QTKSFNEGVSGDFVGIGAEMQKKNDQIMVTSPMKGSPAERAGIRPKDVITKVNGKSIK
                     GKALDEVVKDVRGKENTEVTLTVQRGSEEKDVKIKREKIHVKSVEYKKKGKVGVITIN
                     KFQNDTSGELKDAVLKAHKDGLKKIVLDLRNNPGGLLDEAVKMANIFIDKGKTVVKLE
                     KGKDTEAIQTSNDALKEAKDMDISILVNEGSASASEVFTGALKDYNKAKVYGSKTFGK
                     GVVQTTREFKDGSLLKYTEMKWLTPDGHYIHGKGIKPDVTIDTPKYQSLNVIPNTKTF
                     KVGDDDKNIKTIKIGLSALGYKVDNESTQFDKALENQVKAFQQANKLEVTGEFNKETN
                     NKFTELLVEKANKHDDVLDKLIN"
BASE COUNT          505 a          145 c          238 g          358 t
ORIGIN      
        1 attgaaaatg tgtataaaat cttaaatagt gattattaca aaaaacagga ctctgacaag
       61 ttaagtaaag ctgcaattga tggcatggtc aaagaattaa aagatcctta ttctgaatat
      121 ttaacaaaag aacaaacgaa atcctttaat gaaggtgttt caggtgattt tgtaggtatt
      181 ggtgcagaaa tgcaaaagaa aaatgatcaa attatggtta ctagtcctat gaagggatct
      241 ccagcagaac gtgctggcat tcgtcctaaa gatgtcatta ctaaagtaaa tggaaaatca
      301 attaaaggta aagcattaga tgaagttgtc aaagatgttc gtggtaaaga aaacactgaa
      361 gtcactttaa ctgttcaacg aggtagtgaa gaaaaagacg ttaagattaa acgtgaaaaa
      421 attcatgtta aaagtgttga gtataagaaa aaaggtaaag ttggagttat tactattaat
      481 aaattccaga atgatacatc aggtgaattg aaagatgcag ttctaaaagc tcacaaagat
      541 ggtttgaaaa agattgtttt agatttaaga aataatccag gtggactact agatgaagct
      601 gttaaaatgg caaatatttt tatcgataaa ggaaaaactg ttgttaaact agaaaaaggt
      661 aaagatactg aagcaattca aacttctaat gatgcgttaa aagaagcgaa agacatggat
      721 atatccatct tagtgaatga aggttcagct agcgcttctg aagtgtttac tggtgcgcta
      781 aaagactata ataaagctaa agtttatggg tcaaaaacat tcggcaaagg tgtcgtacaa
      841 actacaagag agtttaagga tggttcattg ttaaaatata ctgaaatgaa atggttaacg
      901 ccagatggtc attatattca cggtaaaggc attaaaccag acgttactat tgacacacct
      961 aaatatcaat ctttaaatgt cattcctaat acgaaaacat ttaaagttgg agacgatgat
     1021 aaaaatatta aaactattaa aattggttta tcagctttag gttataaagt tgataatgaa
     1081 tcaacgcaat ttgataaagc tttagaaaat caagttaaag ctttccaaca agcgaataaa
     1141 cttgaggtaa ctggtgaatt taataaagaa acgaataata aatttactga gttattagtt
     1201 gaaaaagcta ataaacatga tgatgttctc gataagttga ttaata
//
LOCUS       DAKTIO010000327         1193 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.339, whole genome shotgun sequence.
ACCESSION   DAKTIO010000327 DAKTIO010000000
VERSION     DAKTIO010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.339"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            48..533
                     /locus_tag="O0293_002865"
     CDS             48..533
                     /locus_tag="O0293_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCY0281673.1"
                     /translation="MRKFLSKTHHHTNPLWRVYRLVKFSKVFKNVIIIEFSKFIPSMV
                     LKRHIYKQLLNINIGNQSSIAYKVMLDIFYPELITIGSNSVIGYNVTILTHEALVDEF
                     RYGPVTIGSNTLIGANATILPGITIGDNVKVAAGTVVSKDIPDNGFAYGNPMYIKMIR
                     R"
     gene            541..>1193
                     /locus_tag="O0293_002866"
     CDS             541..>1193
                     /locus_tag="O0293_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCY0281674.1"
                     /translation="MAQKNNNVIPMTFDDAFYRKMAKQKFKQREYKRAAEYFEKVLEL
                     SPDDMEIQIDYAQCLVQLGIAKKAEHLFYDNIIYNRHLEDSFYELSQLNIEVNEPNKA
                     FLFGINYVIVSDDQDYRDELDQMFDVKYQSEEQIELEAQLFVVQILFQYLFSQGRLKD
                     AKNYVLHQPQEVQDHRVVRNLLAMCYLYLGEYDTAKALYEALLQEDSTDIYALCHYT"
BASE COUNT          425 a          157 c          218 g          393 t
ORIGIN      
        1 agttggggcg cttccatggc caacaaaaaa agtgaagtag tgatagtttg aggaaatttt
       61 tatcaaaaac acatcatcat acaaaccctt tatggcgtgt ataccgtctt gttaaatttt
      121 cgaaagtttt taagaatgta attatcattg aattttcgaa atttattcca agtatggtac
      181 tgaaaagaca tatatataaa caacttttaa atattaatat cggtaatcaa tcgtcgatag
      241 cttataaagt aatgttagat attttttacc cagaactgat tacgattggt agtaacagtg
      301 ttattggtta caatgtaaca attttgacgc atgaagcatt agttgatgaa tttcgttatg
      361 gaccagtgac gataggatct aacactttga ttggtgcaaa tgctaccatt ttacccggta
      421 taacgattgg tgacaatgta aaagttgcag ctggtacggt tgtttcaaaa gatataccgg
      481 ataatggatt tgcatatggc aaccctatgt atataaaaat gattaggagg tgacaatttt
      541 atggcgcaaa agaataataa tgtaattcca atgacttttg atgatgcatt ttatcgtaaa
      601 atggctaaac agaagtttaa acaaagagaa tataaacgag ctgctgaata ctttgaaaaa
      661 gtgttagaat tgtcacctga tgatatggaa attcaaattg attatgcaca atgtctagtg
      721 caacttggta ttgctaaaaa agcagaacat ttattttatg acaatattat ttataatagg
      781 catctagaag atagctttta tgaattgagt cagctcaaca ttgaagttaa cgaaccaaac
      841 aaggcattct tgtttggtat taattatgtt attgttagcg acgaccaaga ttatagagat
      901 gaattagatc aaatgtttga tgtgaaatat caaagtgaag aacaaattga acttgaagct
      961 caattgtttg tagttcaaat actattccaa tatctttttt ctcaaggtcg attaaaagat
     1021 gcaaagaatt atgtcttaca tcaaccacaa gaagttcaag atcatcgtgt agtacgtaat
     1081 ttattggcaa tgtgttattt atatctcggt gaatatgata cggctaaagc attgtacgaa
     1141 gcactattac aagaggatag tacagatata tatgcattat gccattatac ttt
//
LOCUS       DAKTIO010000328         1193 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.340, whole genome shotgun sequence.
ACCESSION   DAKTIO010000328 DAKTIO010000000
VERSION     DAKTIO010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.340"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..218)
                     /locus_tag="O0293_002867"
     CDS             complement(<1..218)
                     /locus_tag="O0293_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0281675.1"
                     /translation="MKREVILKLQQFIIERENADKRRQYKKENEELDLSLTQFHIIEL
                     IDNNDKVNNKFLSEMLNVSKPAITKSIK"
     gene            652..>1193
                     /locus_tag="O0293_002868"
     CDS             652..>1193
                     /locus_tag="O0293_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0281676.1"
                     /translation="MDVLTIEHLTKKIGNKTILEDVSFKLKRGQIVGLVGANGAGKTT
                     LMKVILGYSSFQSGNFNVINSKDSKSNIGALIENPGIYPFMSGYENLKLLNESKNTQD
                     IDKIVSQLHMDEYIHKKAKTYSLGMKQKLGIAIAFLNKPQFIILDEPMNGLDPKAVRD
                     VRELIVQKAQEGVTFLISSH"
BASE COUNT          415 a          184 c          159 g          435 t
ORIGIN      
        1 ttttttatcg atttagtaat tgctggtttt gatacattta acatttcaga caaaaattta
       61 ttattcactt tatcattatt atcaatcaac tctatgatat gaaattgtgt tagagataaa
      121 tctagttcct cattttcctt tttatactgt cttcttttat cggcattttc tctttctata
      181 ataaattgtt gtaattttaa aataacttct ctcttcatat ccactattat tcctcctctt
      241 cattagttaa ccggttaact atattattgt tgtcactccc atttttgtca aatattgttg
      301 taattaacat atatttactt agttatgcac catttattcc gcatttcaca aatccaccta
      361 taatacaaac tatcatttta aataagcaga atttaccctg tcttccacac aaattgtttt
      421 cgacacttta atgtgcctac tatccaaaaa agttactcat aacaaattgc gcattttaat
      481 aaatgtagca atgccatttg catgctgcaa aaatactcgt attcatattt atgttttata
      541 ttattaaagt tttttaaatc catattttta taacacttgc tatgtgataa aattaatttt
      601 atatataaaa tcttaagatt cagattattt aatagcaaag gagatagtga tatggatgtt
      661 ttaacaatag aacatttaac aaagaagata ggcaacaaaa cgattctcga agatgtatca
      721 tttaagctga aacgcggaca aatagttggt ctcgttggag cgaatggtgc aggtaaaaca
      781 actttaatga aagttatatt aggttactct agtttccaaa gcgggaattt taatgttatt
      841 aacagcaagg acagcaaaag caatatcggc gcattgattg aaaatccagg aatatatcct
      901 tttatgtctg gatatgaaaa cttgaagtta ttgaatgaat caaaaaacac tcaagatatc
      961 gataaaattg tctcacaact tcatatggat gaatacattc ataaaaaagc taaaacgtat
     1021 tctcttggta tgaaacaaaa attaggaatt gctatagcat ttttaaataa acctcaattc
     1081 attatcttag atgaaccaat gaatggctta gatccaaaag ctgtgcgaga tgtacgtgaa
     1141 ttgattgtcc aaaaagcgca agaaggtgtt actttcttaa tttcgagtca tat
//
LOCUS       DAKTIO010000329         1172 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.341, whole genome shotgun sequence.
ACCESSION   DAKTIO010000329 DAKTIO010000000
VERSION     DAKTIO010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.341"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            6..365
                     /locus_tag="O0293_002869"
                     /pseudo
     CDS             6..365
                     /locus_tag="O0293_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(456..>1172)
                     /gene="sdpB"
                     /locus_tag="O0293_002870"
     CDS             complement(456..>1172)
                     /gene="sdpB"
                     /locus_tag="O0293_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpB"
                     /protein_id="HCY0281677.1"
                     /translation="YTFKDIAWRDLLIIPIIGVIFYALTIAAYIGPILFGYYEIDSNL
                     IFVATITQALAYTIGIGAFYLFHLKVMPSRLRAGFAYIKKYWLRLSITYIIALILIYT
                     YEYMTQFLPKHLQYSETANELELNKMFEVPAFLPVAFLLIVIVGPIVEEIVFRHILIG
                     ELGKKFNFIAMSIVSVFLFAFIHVTDAKSPFEFGPYLILSIILVFTYLKSGRNLGSTI
                     ALHIANNFVSFVISVISIYA"
BASE COUNT          445 a          200 c          154 g          373 t
ORIGIN      
        1 aaaaaataaa acactttgcc caacttacac taccaataga aactgctgtt agaattcctc
       61 aaaatgatat ttcgcgatat gttaatgaaa ttgttaaaaa gatagctgat agcgaattcg
      121 atgaattcag acatcatcgt ggcgcaacat cctatcatct aaaaatgatg ttaaaaatca
      181 cctcatattc atatactcaa tctgaatttt ctggccgtag aatagaaaaa ttacttcata
      241 acagtattcg aatgatgtgg ttagctcaag atcaaacacc ttcttataaa actattaatc
      301 tttttagagt gaatcctaat actgatgcgc taattgaatc tttatttatt cagtttcata
      361 ataaaatgca tatcaaaaaa gctgatttct atcaaataat taatagaaat cagctttttt
      421 cattgcctaa aaacttaatg tcccgacctc tttatctacg cataaatact tattactgat
      481 ataacgaaag aaacaaaatt atttgctata tgtaatgcaa ttgttgaacc taggtttctt
      541 ccagatttta aataagtgaa aactaatatg atggatagta tgagatatgg accaaactca
      601 aacggcgact ttgcatcagt cacatgaata aatgcaaata agaacaccga aacaatactc
      661 atagctataa aattaaactt cttacctaat tctccaatta aaatatgtct aaatacgatt
      721 tcttcaacta ttggacctac aatcacaatt aataagaatg ctacaggtaa aaatgcaggc
      781 acttcaaaca ttttatttag ctcaagttca ttggctgttt cactatattg caaatgttta
      841 ggtagaaact gtgtcatata ttcatatgta taaattaaga tgagagcaat aatatacgtt
      901 attgacaatc taagccaata ttttttgata tacgcaaaac cagctcgaag ccttgatggc
      961 atcactttta aatgaaataa ataaaatgcg ccaatcccaa tcgtatatgc taaagcttgt
     1021 gtgatagtcg ctacaaatat cagattacta tcgatttcat aataaccaaa caaaattggt
     1081 cctatgtaag ctgcaattgt gagtgcataa aatataacac ctataattgg aattataagc
     1141 aaatctctcc atgctatatc tttaaacgtg ta
//
LOCUS       DAKTIO010000330         1170 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.342, whole genome shotgun sequence.
ACCESSION   DAKTIO010000330 DAKTIO010000000
VERSION     DAKTIO010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.342"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            43..921
                     /locus_tag="O0293_002871"
     CDS             43..921
                     /locus_tag="O0293_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HCY0281678.1"
                     /translation="MLNIQDVSHLSKKEQKAYNRFVESVENGNLPVLPCIEMDLKEMQ
                     EETLNQSKIGGMPFLKYFKDIPLDENNVPMVLLAQINLDDLPEQQELFPVKEGILQFW
                     ISSEDQMYGMSENLKGNNINSRLVYIKEPITDLSLENIQAHLKSLDADNEDIPFSGAF
                     SIEFRLSKQTITCTDYKYDEDVLALWNKVNPSFALKSMFGGYDELMEPVCNTFTAKEP
                     FNQLGGYPYFDQIDPRTNDQELKMYDRVLLQIDSTRDGNSSIIWGDLGIANILVKSTD
                     LEAMKFDDYMYSWDCS"
BASE COUNT          406 a          170 c          225 g          369 t
ORIGIN      
        1 tgaatttagc tattttaaaa ataaaaattg ggggaagtta atatgctaaa cattcaagac
       61 gttagtcatc tttctaaaaa ggagcaaaaa gcatataacc gtttcgtaga atctgtagaa
      121 aacggtaatt taccagtact accatgtatt gaaatggatc taaaagagat gcaagaagaa
      181 acattaaacc agagtaagat tggtggaatg ccatttttaa aatattttaa agatatacca
      241 ttagatgaaa ataatgtacc aatggtattg ctagcacaga ttaatttgga tgatcttcca
      301 gaacaacaag aattatttcc tgtaaaagaa gggatattgc agttttggat tagttcagaa
      361 gatcaaatgt atggaatgtc tgaaaattta aagggaaaca atataaactc aaggcttgtt
      421 tatataaaag agccaattac agatttatca ctcgaaaata ttcaagcgca tttgaagtca
      481 ttagatgctg ataatgagga tatcccgttc agtggagcat tttctataga atttagattg
      541 tcgaaacaaa ctattacatg tactgattat aagtacgatg aggacgtgct tgcattgtgg
      601 aataaagtca atccatcctt cgcgctaaaa tcaatgtttg gtggttatga tgaattgatg
      661 gaacctgtgt gtaacacatt tactgctaag gaaccattta atcaacttgg tggttatcca
      721 tattttgacc aaatagatcc aagaacgaac gatcaagaac tgaaaatgta tgatagagtc
      781 ttactgcaaa ttgattctac aagagatggt aattcttcga ttatatgggg tgatttaggt
      841 attgccaata tcttagtgaa atctactgac cttgaggcta tgaagtttga tgattacatg
      901 tattcatggg attgcagcta agagaatcat aaaaatatat tactttaata agtgtagtat
      961 ctatagatca atctagggag tgggacataa atgatatttt cgcaaaattt atttcgtcgt
     1021 cccaccccaa cttgcattgt ctgtagaaat tgggaatcca atttctcttt gttggggccc
     1081 atccccaact tgcattgcct gtagaatttc ttttcgaaat tctctttgtt ggggcccctg
     1141 actagaattg aaaaaagctt gttacaagcg
//
LOCUS       DAKTIO010000331         1160 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.343, whole genome shotgun sequence.
ACCESSION   DAKTIO010000331 DAKTIO010000000
VERSION     DAKTIO010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.343"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..>1160
                     /gene="fmhC"
                     /locus_tag="O0293_002872"
     CDS             <1..>1160
                     /gene="fmhC"
                     /locus_tag="O0293_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhC"
                     /protein_id="HCY0281679.1"
                     /translation="HYTQSIELYNYRNKINHEAHIVGVKNDKNEVIAACLLTEARIFK
                     FYKYFYSHRGPLLDYFDAKLVCYFFKELSKFIYKNRGVFILVDPYLIENLRDANGRII
                     KNYNNSVIVKMLGKIGYLHQGYTTGYSNKSQIRWISVLDLKDKDENQLLKEMEYQTRR
                     NIKKTIEIGVKVEDLSIEETNRFYKLFQMAEEKHGFHFMNEDYFKRMQEIYKDKAMLK
                     IACINLNEYQDKLKIQLLKIENEMMTVNRALNENPNSKKNKSKLNQLNMQLSSINNRI
                     SKTEELIFEDGPVLDLAAALFICTDDEVYYLSSGSNPKYNQYMGAYHLQWHMIKYAKS
                     HNINRYNFYGITGVFSNEADDFGVQQFKKGFNAHVEELIGDFIKPVRPILYK"
BASE COUNT          479 a          111 c          188 g          382 t
ORIGIN      
        1 cattatacac agtctataga attatataat tatagaaata aaataaatca tgaagcacat
       61 attgtgggag tgaagaatga taaaaatgaa gttatagctg catgtttatt aacagaggca
      121 cgaattttta aattctacaa atatttctac tctcatagag gtcctttact tgattatttc
      181 gatgctaaat tagtttgtta cttttttaaa gaattatcta aattcattta taaaaataga
      241 ggagtattta ttcttgttga tccatattta atagagaatt taagagatgc aaatggtagg
      301 ataataaaga attataataa ttcagtgata gtaaagatgc tagggaaaat tgggtatctc
      361 catcaaggtt atacaacagg atattcaaat aaaagtcaaa ttaggtggat ttctgtattg
      421 gatttaaaag ataaagatga gaatcaactt ttaaaagaaa tggaatacca aactagaaga
      481 aatataaaaa agactattga gattggtgtt aaggttgaag atttatctat tgaagaaaca
      541 aatcgatttt ataaattgtt tcaaatggct gaagaaaaac atggttttca tttcatgaat
      601 gaagattatt ttaaacgaat gcaagaaata tataaagata aggcaatgtt aaagatagct
      661 tgtataaatc ttaatgaata tcaagataaa ttaaaaatac aattattgaa aatcgaaaat
      721 gaaatgatga ctgtgaacag agcattaaat gaaaatccaa attctaaaaa aaataaatca
      781 aaattaaatc agttaaatat gcaattatct agtattaata atagaattag taaaaccgaa
      841 gaactaatat ttgaagatgg acctgttttg gatttagctg ctgctttatt tatatgtact
      901 gatgatgaag tttattatct atcaagtgga tcaaatccga aatataatca gtatatgggt
      961 gcatatcatc tacaatggca tatgataaaa tatgcaaaat cacataatat taataggtat
     1021 aatttttatg gaataacagg cgtctttagt aatgaggcgg atgattttgg tgttcaacaa
     1081 tttaaaaagg gttttaatgc acatgttgaa gaattaattg gtgatttcat caaaccagta
     1141 agaccaattc tatataaatt
//
LOCUS       DAKTIO010000332         1157 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.344, whole genome shotgun sequence.
ACCESSION   DAKTIO010000332 DAKTIO010000000
VERSION     DAKTIO010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.344"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            112..>1157
                     /locus_tag="O0293_002873"
     CDS             112..>1157
                     /locus_tag="O0293_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HCY0281680.1"
                     /translation="MSNANNADNKQFFSKILNSIGAGVVIALVPNALLGEVLKIFKSG
                     NEILELTYQLVILIQSFMAFIIGVLAAHQFKFNGAGAAIVGTSAMIGSGAVVYSNNSF
                     MLKGIGDIINTSLVVIIACLIYMVLQNKLGSFELIILPVLVPIVSGGIGLITLPYIRK
                     ITQAIGNVIHSFTDLNPLLMSILISVAFSLLMVTPISLVAIATAISLNGLGSGAANLG
                     IVAACVTFLFGSLRVNSIGVNAVLLIGAAKMMIPVYLKNLIISIPLTINGIITGIIAY
                     VLQVKGTPLSAGFGYTGLVGPINAFNRMSGDPTMNIILLALGYFVIPFVSAFIVHELC
                     KKFIPIYSNDIYKF"
BASE COUNT          356 a          145 c          209 g          447 t
ORIGIN      
        1 gctttattca atttgtggat tgttatattt agattaaatt agccaactaa tcattgttaa
       61 agttaatttt ctataatatg atgagtttaa tgttcaaatt ggaggtcgtt tatgagtaac
      121 gcaaataatg ctgataataa acagtttttc agtaagattt taaattctat tggtgctggt
      181 gttgttattg cacttgttcc gaatgcttta ttaggtgaag tattaaaaat atttaaaagt
      241 ggtaatgaaa ttttagaatt aacgtatcag ttagtgatac taattcaatc atttatggca
      301 tttattattg gggttttagc tgctcatcaa tttaaattta atggtgcagg tgctgctata
      361 gtaggtacat cagctatgat tggttcaggt gctgttgtat atagcaataa tagttttatg
      421 ttaaaaggta taggtgacat tataaatacg agtttggttg ttattattgc atgtcttata
      481 tatatggtat tacaaaacaa acttggatct tttgaattaa ttatattacc agttttagta
      541 cctatcgtta gtgggggtat cggtctaatc acattacctt acattcgaaa aattactcaa
      601 gcaattggaa atgtaattca ttcttttaca gacttaaatc cgttgttaat gtctatactt
      661 attagtgtgg cattttcatt attaatggta acaccaatat cgttagtagc gattgcaact
      721 gcgattagtt taaacggact aggaagtgga gcagcaaacc taggtatagt ggctgcctgt
      781 gttactttct tatttggctc attgcgtgtc aattctattg gtgtaaatgc agttttactc
      841 ataggagctg caaaaatgat gatcccagtt tacttgaaaa atttaattat ttcgatacca
      901 ttgactataa atggaataat tactggaatc attgcttacg ttcttcaggt taaaggaaca
      961 ccattgtctg ctggatttgg atatacagga ttagttggcc caataaatgc atttaaccga
     1021 atgtcaggtg atccaacaat gaatattatt ttattagcac ttggttattt tgtcatacca
     1081 tttgtatcgg catttattgt tcatgaattg tgtaaaaaat tcattcctat atattctaat
     1141 gatatttata aatttga
//
LOCUS       DAKTIO010000333         1121 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.345, whole genome shotgun sequence.
ACCESSION   DAKTIO010000333 DAKTIO010000000
VERSION     DAKTIO010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.345"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..759
                     /locus_tag="O0293_002874"
     CDS             <1..759
                     /locus_tag="O0293_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0281681.1"
                     /translation="LVLLIILIVGYVIMTKTNGRNAQIKDTFNQTLKLYPTKNLDDFY
                     DKEGFRDQEFKKGDKGTWIVNSEMVIEPKGKDMETRGMVLYINRNTRTTKGYYFISEM
                     TDDSNGRPKDDEKRYPVKMEHNKIIPTKPLPNDKLKKEIENFKFFVQYGNFKDINDYK
                     DGDISYNPNVPSYSAKYQLNNDDYNVQQLRKRYDIPTKQAPKLLLKGDGDLKGSSVGS
                     RSLEFTFVENKEENIYFTDSVQYTPSEDTRYESN"
     gene            complement(763..885)
                     /locus_tag="O0293_002875"
     CDS             complement(763..885)
                     /locus_tag="O0293_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281682.1"
                     /translation="MSYIIKFTFKFFEMATYISQLTLDIELVSSELLVILPGFI"
BASE COUNT          459 a          150 c          200 g          312 t
ORIGIN      
        1 ttggtattac tcattatatt gattgtaggt tatgtcatta tgacaaaaac aaatggtcga
       61 aacgcccaaa ttaaagacac atttaatcaa acattaaaat tatatccaac caaaaatctc
      121 gatgattttt acgataaaga aggctttcga gatcaagaat ttaaaaaggg tgataaaggt
      181 acttggatag ttaattctga aatggtaatc gagccaaaag gtaaggatat ggaaacgaga
      241 ggaatggtgc tctatatcaa tcgcaatact agaaccacaa aagggtatta ttttataagt
      301 gaaatgacag atgacagtaa cggcagacca aaggatgatg aaaaaaggta tccggtaaaa
      361 atggaacata ataaaatcat accaacgaag ccactaccga atgacaagtt aaaaaaagag
      421 attgaaaact ttaagttctt tgtacaatat ggcaacttta aagatattaa tgattataaa
      481 gatggtgata tttcatataa tcctaatgta ccaagttatt cggcaaaata tcaattgaat
      541 aatgatgatt ataatgtcca acagttaaga aaaagatatg atattccaac caaacaagcg
      601 ccgaaactat tattgaaagg cgatggagat ttaaaaggtt catccgtagg ttctagaagt
      661 cttgaattta cctttgtcga aaataaagaa gaaaacattt attttacaga ttctgtacaa
      721 tatactccaa gtgaggatac aaggtatgag tcaaactgaa tatcaaataa aacctggtaa
      781 tataacaagt aactctgaag aaacaagttc gatatctaaa gtgagctgtg aaatataggt
      841 agccatttca aaaaatttaa aggtgaattt gataatgtag ctcaaggaga ttgggttaaa
      901 aaggcgaaga atgaactgga tgatattagt aagaaattaa aaaatattca aagaacggaa
      961 gtttaatagc ttatatgatt cttggagcta agacagcatg cgttcattca tgccattatt
     1021 aatataagca ccgcaacaaa aaagcttcta atgtgataca ggaacctcat attccgtatc
     1081 atgttagaag cttttaatgt ctaaagaaca tctacatttt a
//
LOCUS       DAKTIO010000334         1117 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.346, whole genome shotgun sequence.
ACCESSION   DAKTIO010000334 DAKTIO010000000
VERSION     DAKTIO010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.346"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(119..1117)
                     /locus_tag="O0293_002876"
     CDS             complement(119..1117)
                     /locus_tag="O0293_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HCY0281683.1"
                     /translation="MIIVTIAVVIFSLIFDFINGFHDTANAVATAVSTRALTPKTAIL
                     MAAVMNFIGALTFTGVAGTITKDIVDPFKLENGLVVVLAAILAAIIWNLATWFYGIPS
                     SSSHALIGSIAGAAIASEGSFGVLHYQGFTKIIIVLIVSPIIAFCVGFLMYSIFKVIF
                     KNANLTRANRNFRFFQIFTAALQSFSHGTNDAQKSMGIITLALIVANVQNDGSVEPQL
                     WVKFACATAMGLGTAIGGWKIIKTVGGNIMKIRPANGAAADLSSALTIFVASSLHFPL
                     STTHVVSSSILGVGASNRAKGVKWSTAQRMIITWVITLPISALLAGLLFYILNLFF"
BASE COUNT          382 a          214 c          188 g          333 t
ORIGIN      
        1 tttgtcttgg tcattctacg taaattctat aaaatatgtt atctacttac ataaaaaact
       61 gaacttcaat accaccatat gtttgtgata ctgaagttca gtttagtttt attttcaatt
      121 agaaaaataa gttaagtata tagaatagta aacctgctaa caatgctgaa ataggtaatg
      181 taatcaccca tgtaatgatc attcgttgcg cagtgctcca ttttacacct ttagctcggt
      241 tagaagcacc aacacctaag attgatgatg acacaacgtg agttgttgat aatgggaaat
      301 gtagcgatga tgcaacaaaa attgttaatg cagatgataa atcggccgca gcaccatttg
      361 ctggacgtat tttcataata ttaccaccta cagttttgat aattttccag ccaccaattg
      421 cagtaccaag ccccattgct gtcgcacagg caaattttac ccataactgt ggttcaacac
      481 tgccatcatt ctgtacatta gcgacaatca atgccaacgt aataataccc attgattttt
      541 gcgcatcatt cgtaccgtga gagaatgatt gtaacgctgc tgtgaaaatt tggaaaaatc
      601 taaagttacg attcgctctt gttaaatttg catttttaaa gataacttta aaaattgaat
      661 acatcaagaa accaacacaa aatgcgataa tcggtgaaac gattaataca ataataattt
      721 ttgtgaaacc ttggtaatgt aacactccaa atgagccttc agatgcgatt gctgcacccg
      781 caattgaacc tataagtgca tgtgaagacg aacttggaat tccgtaaaac caagtagcta
      841 aattccaaat aatagccgca agtattgcag ctaacacaac aactaatcca ttttccaatt
      901 taaatggatc gacaatgtct ttagtaatgg tgcctgcaac gcccgtaaat gttaaagcac
      961 ctataaagtt catcactgct gccattaaaa ttgccgtttt aggcgttaac gctctagtag
     1021 atacagcagt agctactgca ttggctgtat catggaatcc attgataaag tcaaatatca
     1081 gcgagaaaat aactacagct atagtgacga tgattat
//
LOCUS       DAKTIO010000335         1108 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.347, whole genome shotgun sequence.
ACCESSION   DAKTIO010000335 DAKTIO010000000
VERSION     DAKTIO010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.347"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..382)
                     /locus_tag="O0293_002877"
     CDS             complement(<1..382)
                     /locus_tag="O0293_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281684.1"
                     /translation="MLGKYWIQLLIATVIISLISIKAFPLAIGALYLPVIFKVIKLQL
                     NLSKGLIDDVNAQTFIKSNQSGIVISVICCLLITAILYYTLDGFYASLTGVLGTLVAL
                     NPYTTIVSAVLYILTAIATVEATKT"
BASE COUNT          399 a          184 c          175 g          350 t
ORIGIN      
        1 ttgttttagt agcttcaacc gtagcaatag ctgtaagtat atataataca gcactaacaa
       61 ttgtcgtata tggatttaga gcaacaagcg tacctaaaac tcctgttaaa ctcgcataaa
      121 acccatcaag tgtataatac aatattgctg ttattaataa acaacaaatt acactgatta
      181 caatacctga ttgattactt ttaatgaatg tttgcgcatt aacatcatca attaatcctt
      241 ttgataaatt gagttgtaat tttattactt tgaaaataac aggtaaatat aatgccccaa
      301 ttgccaatgg aaaagcttta attgatatta aacttataat aactgttgct atcaataatt
      361 gaatccagta ttttcctaac ataaatatat aaatctcctc taatttcatt cttcaatagc
      421 atatcataat cttggcatat taagaaacgc ggtttaatga tttcattaaa aatattactg
      481 atagatgact tctttcaatt atgtctggag taattaatta tcaattccgt ttaaatggtg
      541 ttttaatatt taaaattgaa cttttgatat attactatgt ctggtacaca aatcaatgtt
      601 ttatgcttta caaagttata ttggcagtag ttgactgcag tccacaacat agaggcttcg
      661 gaatgtcagc ttctatttca tgcaagttgg tggagctcca acatagtgga attggattcc
      721 caatttctac agacattgca aattggggaa acgggccaca aactcagaaa ctggtggaaa
      781 gtcagcttaa aataacatgc aagttggcga ggccccaaaa tagtgagatc ggatttctaa
      841 tttctacaga cattgcaaat tagggaaacg ggccacaaac tcagaagttg gtggaaagtc
      901 agcttaaaat aacatgcaag ttggcggggc cacaacatag aaaaattgga tcctcaattt
      961 ctacaaacaa tgtaagttgg ggaaacagcc ccaacactga aactagcaga aagtcagctt
     1021 ctatgaatat aataaaaaag ctaggtaaca aaatgctacc taacttcata ttcaagataa
     1081 tcaatcctat ttgatatatg ttctatac
//
LOCUS       DAKTIO010000336         1104 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.348, whole genome shotgun sequence.
ACCESSION   DAKTIO010000336 DAKTIO010000000
VERSION     DAKTIO010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.348"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(1..901)
                     /locus_tag="O0293_002878"
                     /pseudo
     CDS             complement(1..901)
                     /locus_tag="O0293_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440612.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
BASE COUNT          402 a          197 c          126 g          379 t
ORIGIN      
        1 accaaaagtc gatttgtttt tgcaattggt tttcatttcc tcttaaagat attttcatta
       61 aatctgtcaa atcaatagac gctatatttt tcaacttatc tctatattta tttttagtac
      121 gtctttctaa atttccccat tcctcttctt cgtgagttaa taaatgaagc attgctcgtt
      181 cttgtatatt ttcaatcatt tttaaattcg gttttaaaat atgcaaatca tcaaaacaat
      241 ctttccaaca atcaaccata tctcgtttta attcaatttc cacacgccat agaaatgttg
      301 aatcaatttc aacatctgca ttatctttac gttcttgttt ttattataaa tccgaataaa
      361 cctatcacta ttacgcacac caaaatattt tgtttctggt tttacattac gtccataaaa
      421 tatagttttc tttaccgact tatctgacaa tgcataatag tcatttaaat caaattcaaa
      481 atcaaaagcc aaatctaatc tcgtaaaact aacatcgtcc aaataactga tgatattttg
      541 ttttaaccaa agcacttcat catgcgaaag cttattagga ttaaattcaa cgcgcatact
      601 acgtctattc caaagagttg cttttatttt gtcatattca atataaactt tttctttaag
      661 agctttagct ttaaagtttg tttgtaaaat atcccaaagc cgaatttcag gattagtact
      721 cataaaatgt gaaagtctct ctgcgttaga catgctaaga ttcccaacaa tcgttatagc
      781 gtcaaaagac aattttggaa tagctagtga catcctatgt cgatttaacc ggctattacc
      841 ggatattaga gtatccagtt ttacaaatgg atgaaacgaa attcaaaaca ctaaaaaata
      901 tgttccacta acagcaaaaa aataccatta tgttcctact aaaaaactaa aaatactgga
      961 gaacaaatgt caggatataa cttaggatac tatgtaataa aaatttacaa taaaaaaaca
     1021 ggaaaacaaa tttcaagtaa aagaataccc atacaaagag gataaaataa aaaacctcga
     1081 actgaaatga tgatcttttc agct
//
LOCUS       DAKTIO010000337         1092 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.349, whole genome shotgun sequence.
ACCESSION   DAKTIO010000337 DAKTIO010000000
VERSION     DAKTIO010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.349"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            77..568
                     /locus_tag="O0293_002879"
     CDS             77..568
                     /locus_tag="O0293_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCY0281685.1"
                     /translation="MVSDFESNDKITEVELLMHYNPKVINSKIKAMRSQIESLYHLNM
                     NHVITNDNDMLVSVSYPLDKLVLYIIEEKDKLEYYMKTAQDRLNLFKNIIKNYSENEQ
                     QDVMRYMLSSGKVKNEGVIERLKVDIYKVESRKRQERQNQREELHRIEFNKHLEQVKK
                     ELS"
     gene            568..720
                     /locus_tag="O0293_002880"
                     /pseudo
     CDS             568..720
                     /locus_tag="O0293_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
BASE COUNT          435 a          118 c          205 g          334 t
ORIGIN      
        1 gaaaggggga taacttgatt tatccctttt tcagatactt ataaatatta aattcgatat
       61 gtaaaggtgt ggttatttgg tatctgactt tgaaagcaat gataaaatca ctgaagtaga
      121 acttttaatg cattacaatc cgaaagtcat taatagcaaa attaaagcga tgagatcaca
      181 aatagaaagc ttatatcact tgaacatgaa ccatgtgatc acgaatgata acgatatgct
      241 ggtatctgta tcatatccat tagataaatt agtactttat attatagaag agaaagataa
      301 acttgaatat tacatgaaaa cagctcagga taggctaaat ttgtttaaaa atatcatcaa
      361 aaactattct gaaaatgaac aacaggatgt tatgaggtac atgttaagta gtgggaaagt
      421 taaaaatgaa ggagtcattg aacgtttaaa agtagatatt tataaagttg aaagtagaaa
      481 gcgtcaagaa cgacaaaacc agcgagaaga attacatcga atagagttta ataagcattt
      541 agaacaagtt aaaaaagaac tttcatagat aagcatgata ttaacgggaa tattcctata
      601 ttcattaata ttggtgaatg ggacggagat gatgaggaat tagataaggc agtgcaagat
      661 gtatctaacg ctaaccataa tcatactgtg attgtggatg atataccgtt agaggattga
      721 agaaaatgaa gctatgctat ttataaatta atactaatta gtttgatacc atagcttatt
      781 tactgagaaa gtagacttaa atgtaacaac accagtgttt attgataata ttggtgggtt
      841 tgaggagtag caataaaata aaggaggtaa ttgtgtaaaa tatctctttt tgttatttct
      901 tatttattta caaccgataa aattaaatgt attatatata taacgatcta gccataactc
      961 tattcgggtt atggctactt ttataggggt aaatttatga agccatttga aagtcataat
     1021 aaacaattga aaattctaag aagaagagga atggaagtac cgagtagtgc taaaagagat
     1081 ttagaaaatg aa
//
LOCUS       DAKTIO010000338         1088 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.350, whole genome shotgun sequence.
ACCESSION   DAKTIO010000338 DAKTIO010000000
VERSION     DAKTIO010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.350"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            221..487
                     /gene="zapA"
                     /locus_tag="O0293_002881"
     CDS             221..487
                     /gene="zapA"
                     /locus_tag="O0293_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCY0281686.1"
                     /translation="MTQFKNKVNVSINDQLFTIVGEDNPEHIRYVAHLVDDKIKELGY
                     KAAGLDTSRKAILTAVNIMHEKVLLEEENRRLKQQIHKLQQREQ"
     gene            488..1009
                     /locus_tag="O0293_002882"
     CDS             488..1009
                     /locus_tag="O0293_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HCY0281687.1"
                     /translation="MVIDFIIIIFFVYFVIVGFRRGFWLSMIHLSATIVSLWIASQFY
                     KSIVERLIVFIPYPKTTAFNTTFAFHFNHLQNRFEAIVAFLMITLFCKFILYLIIVTF
                     DKIIAYQNIHIFSRAMGMIVGVFMTIIVLHFTLYLLALYPNEALQHQLKISIVSHSLI
                     FHIPYLSAFTINL"
BASE COUNT          389 a          141 c          167 g          391 t
ORIGIN      
        1 aaatgttata gtgacacaat ttaaacatga taaagtattg aatagaaaat tctttacatt
       61 atttcattat aaaaagaaca ttaagtcatc tatagagatt gtgttgttgt tttatcataa
      121 ttgcaatata acaatattag tatcaaattc agacaaacta tccagtcata ttttataacg
      181 tgaatttaga ttttaaaaaa aggatcagga gaaattataa atgacacagt ttaaaaacaa
      241 ggtaaatgta tcaattaatg atcagctttt tacaattgtt ggggaagata acccagagca
      301 catacgatat gtagcacatt tagttgatga taaaataaaa gaattagggt ataaagcagc
      361 aggtttagat acttcaagaa aagcaatact aactgctgtg aatattatgc atgaaaaagt
      421 actactagaa gaagaaaatc gacgtttgaa acaacaaatt cacaaattgc agcagcgtga
      481 gcaataaatg gtcattgatt ttatcataat cattttcttt gtgtattttg tcatcgttgg
      541 attcagacga ggtttttggt tatctatgat acatttgagt gcaacgattg tatcattgtg
      601 gattgccagt caattttaca aatctattgt agaaagatta attgtattta ttccatatcc
      661 taaaacaaca gcatttaata caacttttgc gtttcatttt aatcatctac aaaatcgatt
      721 tgaagcgatt gtagcttttt taatgattac attgttttgt aagttcattt tatatctaat
      781 tatcgtaact tttgataaaa taatagcgta tcaaaacatt catattttca gtcgtgcaat
      841 gggaatgata gttggtgtgt ttatgacgat aattgtctta cactttacgt tatatctatt
      901 ggcattatat cctaacgaag cattacaaca tcagcttaaa atatctattg tgagtcattc
      961 attgattttt cacatcccat atttatcggc tttcaccatt aatttataaa ttatcataag
     1021 aggtcaggac aaaggtatat attaatgata cgtcctggtc ttctttttat ggagtgttat
     1081 tatgacaa
//
LOCUS       DAKTIO010000339         1083 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.351, whole genome shotgun sequence.
ACCESSION   DAKTIO010000339 DAKTIO010000000
VERSION     DAKTIO010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.351"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(71..979)
                     /gene="rocF"
                     /locus_tag="O0293_002883"
     CDS             complement(71..979)
                     /gene="rocF"
                     /locus_tag="O0293_002883"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HCY0281688.1"
                     /translation="MTKTKAIDIIGAPSTFGQRKLGVDLGPTAIRYAGLISRLKQLDL
                     DVYDKGDIKVPAVNIEKFHSEQKGLRNYDEIIDVNQKLNKEVSASIENNRFPLVLGGD
                     HSIAVGSVSAISKHYNNLGVIWYDAHGDLNIPEESPSGNIHGMPLRILTGEGPKELLE
                     LNSNVIKPENIVLIGMRDLDKGERQFIKDHNIKTFTMSDIDKLGIKEVIENTIEYLKS
                     RNVDGVHLSLDVDALDPLETPGTGTRVLGGLSYRESHFALELLHQSHLISSMDLVEVN
                     PLIDSNNHTAEQAVSLVGTFFGETLL"
BASE COUNT          357 a          207 c          127 g          392 t
ORIGIN      
        1 aatcatttta gtgaattaaa aaaagtagtg caaaaagcaa aatatacttt atacactaca
       61 aatcatttat ttataataaa gtttcaccaa aaaatgttcc aactaatgaa accgcttgtt
      121 cagcagtatg attattactg tcaatcaatg gatttacttc aactaaatcc attgaggaaa
      181 ttaaatgtga ttgatgcagt aattccaatg caaaatggct ttctctataa ctaagaccac
      241 ccaaaactct agtaccagtg cctggcgttt caagcggatc taaagcatca acatctaaag
      301 ataaatgaac gccatcaaca ttgcgtgact tcaaatattc tattgtattt tcaattactt
      361 cctttatccc caatttatca atatctgaca tagtaaatgt tttaatatta tgatctttga
      421 taaattgtct ttcaccttta tctaaatctc tcataccaat tagtacgatg ttttctggct
      481 tgattacatt actatttaat tctaaaagtt ctttgggacc ttcgcctgtc aaaatcctta
      541 gaggcatacc atgaatattt ccacttggtg actcttcagg tatatttaaa tcaccatgtg
      601 catcatacca aataacacct aaattattat aatgtttact tattgctgat actgaaccta
      661 ccgcaataga atgatctcca ccaagaacta gaggaaatct gttattttca attgatgctg
      721 aaacctcttt atttaatttt tgattaacat ctataatttc atcataattt cttaatcctt
      781 tttgttcact atgaaatttt tcaatgttca cagcaggtac cttaatatcc cccttgtcat
      841 atacatcaag gtctaattgc tttaatcttg aaattaatcc agcatatcta attgctgttg
      901 gtcctaaatc aacacctaat tttctttgtc caaatgttga tggtgcacct ataatatcaa
      961 ttgcttttgt ctttgtcata agcgtccccc tttgctctat gtaattaaag aataatgtat
     1021 gcgcttacca ttatcaagca atagctacac atataatctg tttatcttat tacttcataa
     1081 aaa
//
LOCUS       DAKTIO010000340         1068 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.352, whole genome shotgun sequence.
ACCESSION   DAKTIO010000340 DAKTIO010000000
VERSION     DAKTIO010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.352"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            137..1057
                     /gene="rnz"
                     /locus_tag="O0293_002884"
     CDS             137..1057
                     /gene="rnz"
                     /locus_tag="O0293_002884"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCY0281689.1"
                     /translation="MEVTFFGTSAGLPTKERNTQAIALNLEPYSNSIWLFDVGEGTQH
                     QILHHAIKLGKVTHIFITHMHGDHIFGLPGLLSSRSFQGGEQKPLTLVGPKGIKAYVE
                     MSMNLSESHLNYPITYIEIDDHLTYHHDGFTVEAHLLNHGIPSYGYRVMAPETTGTIN
                     VEALKNIGLEPGPKYQEVKSHDTFEHNGQVYQSKDFRGESKQGPVVAIFGDTKPCSNE
                     RVISRDADVMVHEATYIDGEKHLANNYHHSHIEDVFALIKEANVKRTLITHLSNRYNT
                     EDINEIYQTLIQNEDTPNFNFVKDFDSFKI"
BASE COUNT          395 a          151 c          191 g          331 t
ORIGIN      
        1 tagttatgtc tacgttaaag ttagaaaaat taactagcaa agacgaattt ttaacagatt
       61 ttgattcaag tataaattaa aactaaattg atacaaattt tatgataaaa tgaattgaag
      121 aaaaggaggg gcatatatgg aagttacatt ttttggaacg agtgcaggtt tgcctacaaa
      181 agagagaaat acacaagcaa tcgccttaaa tttagaacca tattccaatt ccatatggct
      241 tttcgacgtt ggtgaaggta cacagcacca aattttacat catgcaatta aattaggaaa
      301 agtgacacat atatttatta ctcatatgca tggcgatcat atttttggtt tgccaggatt
      361 actttctagt cgttcttttc agggcggtga acagaagccg cttacattgg ttggaccaaa
      421 aggaattaaa gcatatgtgg aaatgtctat gaatttatca gagtcacatt taaactatcc
      481 aattacatat attgaaatcg atgatcatct gacatatcat catgatggtt ttactgtaga
      541 ggcgcattta cttaatcatg gtattccatc atatggatat agagtaatgg cacctgaaac
      601 tacaggtaca atcaatgttg aagcgttaaa aaacattggt cttgaaccag gtcctaaata
      661 tcaagaagta aaatctcatg atacatttga acataatggt caagtttatc aatcaaaaga
      721 ttttagaggt gagtccaaac agggtccagt tgtagcaatt tttggtgata caaaaccatg
      781 ttctaatgaa cgtgtgattt ctagagatgc tgatgtgatg gtacatgaag cgacctatat
      841 agatggtgaa aagcatttag ccaacaatta tcatcatagc catattgaag acgtatttgc
      901 attgataaaa gaagccaatg ttaaacgtac gttgattaca catttgagca atcgctataa
      961 tacagaagat ataaatgaaa tttatcaaac attgatacaa aatgaagata cacctaattt
     1021 taattttgta aaagattttg atagttttaa aatctaatga caaagtaa
//
LOCUS       DAKTIO010000341         1051 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.353, whole genome shotgun sequence.
ACCESSION   DAKTIO010000341 DAKTIO010000000
VERSION     DAKTIO010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.353"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            60..629
                     /gene="agrB"
                     /locus_tag="O0293_002885"
     CDS             60..629
                     /gene="agrB"
                     /locus_tag="O0293_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator AgrB"
                     /protein_id="HCY0281690.1"
                     /translation="MNYFDNKIDQFATYLQKRNNLDHIQFLQVRLGMQVLAKNIGKLI
                     VMYTIAYILNIFLFTLITNLTFYLIRRHAHGAHAPSSFWCYVESIILFILLPLVIVNF
                     HINFLIMIILTVISLGVISVYAPAATKKKPIPVRLIKRKKYYAIIVSLTLFIITLIIK
                     EPFAQFIQLGIIIEAITLLPIFFIKEDLK"
     gene            626..766
                     /locus_tag="O0293_002886"
     CDS             626..766
                     /locus_tag="O0293_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HCY0281691.1"
                     /translation="MNTLFNLFFDFITGILKNIGNIAAYSTCDFIMDEVEVPKELTQL
                     HE"
BASE COUNT          361 a          130 c          145 g          415 t
ORIGIN      
        1 atataatgat aaaagattgt actaaatcgt ataatgacag tgaggagagt ggtgtaaaat
       61 tgaattattt tgataataaa attgaccagt ttgccacgta tcttcaaaag agaaataact
      121 tagatcatat tcaatttttg caagtacgat tagggatgca ggtcttagct aaaaatatag
      181 gtaaattaat tgttatgtat actattgcct atattttaaa catttttctg tttacgttaa
      241 ttacgaattt aacattttat ttaataagaa gacatgcaca tggtgcacat gcaccttctt
      301 ctttttggtg ttatgtagaa agtattatac tatttatact tttaccttta gtaatagtaa
      361 attttcatat taacttttta attatgatta ttttaacagt tatttcttta ggtgtaatct
      421 cagtatatgc tcctgcagca actaaaaaga agcccattcc tgtgcgactt attaaacgaa
      481 aaaaatatta tgcgattatt gttagtttaa cccttttcat tatcacactt atcatcaaag
      541 agccatttgc ccaattcatt caattaggca tcataataga agctattaca ttattaccta
      601 ttttctttat taaggaggac ttaaaatgaa tacattattt aacttatttt ttgattttat
      661 tactgggatt ttaaaaaaca ttggtaacat cgcagcttat agtacttgtg acttcataat
      721 ggatgaagtt gaagtaccaa aagaattaac acaattacac gaataattta aatagagagt
      781 gtgatagtag gtggaattat taaatagtta taattttgtt ttattcgtat taactcaaat
      841 gatattaatg tttacaatac cagctataat tagtggtatt aagtacagta aacttgatta
      901 ttttttcatc atagtaattt cgacattatc gttatttcta tttaaaatgt ttgatagcgc
      961 gtccttaatc atattaactt catttattat tataatgtat tttgtcaaaa tcaaatggta
     1021 ttctattttg ttgattatga cttcgcagat t
//
LOCUS       DAKTIO010000342         1033 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.354, whole genome shotgun sequence.
ACCESSION   DAKTIO010000342 DAKTIO010000000
VERSION     DAKTIO010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.354"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..111
                     /locus_tag="O0293_002887"
     CDS             <1..111
                     /locus_tag="O0293_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499191.1"
                     /note="subunit E of antiporter complex involved in
                     resistance to high concentrations of Na+, K+, Li+ and/or
                     alkali; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HCY0281692.1"
                     /translation="DSKKFFIHSIDVSEKEKDSLLRSIKHYEDLILEVSR"
     gene            108..410
                     /gene="mnhF2"
                     /locus_tag="O0293_002888"
     CDS             108..410
                     /gene="mnhF2"
                     /locus_tag="O0293_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit F"
                     /protein_id="HCY0281693.1"
                     /translation="MIQTITHIMIISSLIIFGIALIICLFRLIKGPTTADRVVTFDTT
                     SAVVMSIVGVLSVLMGTVSFLDSIMLIAIISFVSSVSISRFIGGGHVFNGNNKRNL"
     gene            385..822
                     /gene="mnhG2"
                     /locus_tag="O0293_002889"
     CDS             385..822
                     /gene="mnhG2"
                     /locus_tag="O0293_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit G"
                     /protein_id="HCY0281694.1"
                     /translation="MEITKEIFSLIAAVMLLLGSFIALISAIGIVKFQDVFLRSHAAT
                     KSSTLSVLLTLIGVLIYFIVNTGFFSVRLLLSLVFINLTSPVGMHLVARAAYRNGAYM
                     YRKNDAHTHASILLSSNEQNSTEALQLRAEKREEHRKKWYQND"
BASE COUNT          331 a          140 c          186 g          376 t
ORIGIN      
        1 gactctaaaa agttttttat tcatagtatc gacgtgtcag aaaaagaaaa agatagtttg
       61 ttaagaagta ttaagcatta tgaagactta atattggagg tgtcgcgatg atacaaacaa
      121 taacacatat tatgattatt agttcactca ttatttttgg aattgcatta atcatctgtt
      181 tatttagatt aatcaaggga cctacaacag cagatcgtgt cgttacattt gatacaacaa
      241 gtgctgtcgt aatgtcaatt gtgggtgtgt taagtgtact tatgggcacc gtttctttct
      301 tagattcaat catgctcatt gccattatat cttttgtaag ttctgtttca atatcacgct
      361 ttattggtgg ggggcatgtg tttaatggaa ataacaaaag aaatctttag tcttattgct
      421 gctgtgatgt tgttgttagg tagttttatt gctcttatta gtgcaatagg tatcgtgaaa
      481 ttccaagatg ttttcttaag aagtcacgct gcgacaaaaa gttcaacttt atccgtgtta
      541 ttaactttaa tcggtgtatt aatttatttt attgtgaata caggattttt cagtgtgcgt
      601 ttattactgt cacttgtttt tattaattta acttcaccag tcggcatgca cttagtcgct
      661 cgcgctgctt atcgcaacgg cgcttatatg tatcgaaaaa atgatgctca cacacatgca
      721 tcaatattat taagttcaaa tgaacaaaac tctacagaag cattacaatt acgtgctgaa
      781 aaacgagaag agcatcgtaa gaaatggtat caaaacgatt gaagtgttgg ctaaattgtt
      841 aagcataaca atgcttttga aaatctgttt tcaaaattaa tcattaaaag agtactttga
      901 aattggttag tcgattttaa ggtgctcatt ttttgtatta taagttaatg ttgttatgtg
      961 aaagcaagtt gtttatttat acaaacaatt agtgataaaa taaaattaaa taattgcaaa
     1021 tttaagtact gca
//
LOCUS       DAKTIO010000343         1027 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.355, whole genome shotgun sequence.
ACCESSION   DAKTIO010000343 DAKTIO010000000
VERSION     DAKTIO010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.355"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            19..>1027
                     /locus_tag="O0293_002890"
     CDS             19..>1027
                     /locus_tag="O0293_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HCY0281695.1"
                     /translation="MSIAIIAVGSELLLGQIANTNGQFLSKVFNEIGQNVLEHKVIGD
                     NKKRLESSVRHALEKYDTVILTGGLGPTKDDLTKHTVAQIVGKDLVIDEPSLKYIESY
                     FEEQGQEMTPNNKQQALVIEGSTVLTNHHGMAPGMMVNFENKQIILLPGPPKEMQPMV
                     KNELLSHFINHNRIIHSELLRFAGIGESKVETILIDLIDKQTNPTIAPLAGSHEVYIR
                     LTANADSKEQAQSLIQPVKQEILDRIGEYYYGSDDTLIEQAVIKKIHEPFVIYDGITN
                     GALYHRLKEVDLNDVLKGMINHNENFVDINKPIEQQLKDAVQFVNKLFNVSSAIILLE
                     YD"
BASE COUNT          387 a          137 c          189 g          314 t
ORIGIN      
        1 gatacaaggg ggatataaat gtcaattgcc attattgctg taggctcaga actattgcta
       61 ggtcaaatcg ctaataccaa cggacaattt ctatctaaag tatttaatga aattggacaa
      121 aatgtattag aacataaagt tattggagat aataaaaaac gtttagaatc aagtgtacgt
      181 catgcgctag aaaaatatga tactgttatt ttaacaggtg gcttaggtcc tacgaaagat
      241 gacttaacga agcatacagt ggcccagatt gttggtaaag atttagttat tgatgagcct
      301 tctttaaaat atattgaaag ctattttgag gaacaaggac aagaaatgac acctaataat
      361 aaacaacagg ctttagtaat tgaaggttca actgtattaa caaatcatca tggcatggct
      421 ccaggaatga tggtgaattt tgaaaacaaa caaattattt tattaccagg tccaccgaaa
      481 gaaatgcaac caatggtgaa aaatgaattg ttgtcacatt ttataaacca taatcgaatt
      541 atacattctg aactattaag atttgcggga ataggtgaat ctaaagtaga aacaatatta
      601 atagatctta tcgataaaca gactaatcct acgattgcgc ctttggcggg aagtcatgaa
      661 gtatatatta gattgactgc aaatgccgac tcaaaagaac aagcacaatc attgattcaa
      721 cctgttaaac aagaaattct tgatcgtatt ggagaatatt attatggttc agatgacaca
      781 ttaattgagc aagctgtaat aaagaaaatt catgaacctt ttgtaatata tgatggtatt
      841 actaatggtg ctttatatca tcgattgaaa gaagtggatt taaacgatgt tctaaagggt
      901 atgattaatc acaatgaaaa ctttgttgat attaataaac ctattgagca gcaattaaaa
      961 gatgcagtgc aatttgttaa taaattgttt aatgtgtcat cagcaattat tctattagag
     1021 tatgatg
//
LOCUS       DAKTIO010000344         1009 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.356, whole genome shotgun sequence.
ACCESSION   DAKTIO010000344 DAKTIO010000000
VERSION     DAKTIO010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.356"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..>1009)
                     /locus_tag="O0293_002891"
     rRNA            complement(<1..>1009)
                     /locus_tag="O0293_002891"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          230 a          298 c          196 g          285 t
ORIGIN      
        1 taaaacgatt ataggtggta caggaatatc aacctgttat ccatcgccta cgcctgtcgg
       61 cctcagctta ggacccgact aacccagagc ggacgagcct tcctctggaa accttagtca
      121 atcggtggac gggattctca cccgtctttc gctactcaca ccggcattct cacttctaag
      181 cgctccacat gtccttacga tcatgcttca acgcccttag aacgctctcc taccattgtc
      241 caaaggacaa tccacagctt cggtaatatg tttagccccg gtacattttc ggcgcagtgt
      301 cactcgacta gtgagctatt acgcactctt taaatgatgg ctgcttctaa gccaacatcc
      361 tagttgtctg ggcaacgcca catccttttc cacttaacat atattttggg accttagctg
      421 gtggtctggg ctgtttccct ttcgaacacg gaccttatca cccatgttct gactcccaag
      481 ttaaattaat tggcattcgg agtttgtctg aattcggtaa cccgagaggg gcccctcgtc
      541 caaacagtgc tctacctcca ataatcatca cttgaggcta gccctaaagc tatttcggag
      601 agaaccagct atctccaggt tcgattggaa tttctccgct accctcagtt catccgctca
      661 cttttcaacg taagtcggtt cggtcctcca ttcagtgtta cctgaacttc aacctgacca
      721 agggtagatc acctggtttc gggtctacga ccaaatacta aacgccctat tcagactcgc
      781 tttcgctacg gctccacatt tactgcttaa ccttgcatca aatcgtaact cgccggttca
      841 ttctacaaaa ggcacgccat cacccattaa cgggctctga ctacttgtaa gcacacggtt
      901 tcaggttcta tttcactccc cttccggggt gcttttcacc tttccctcac ggtactggtt
      961 cactatcggt cactagagag tatttagcct taggagatgg tcctcccag
//
LOCUS       DAKTIO010000345          978 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.357, whole genome shotgun sequence.
ACCESSION   DAKTIO010000345 DAKTIO010000000
VERSION     DAKTIO010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.357"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..694
                     /gene="sarS"
                     /locus_tag="O0293_002892"
     CDS             <1..694
                     /gene="sarS"
                     /locus_tag="O0293_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarS"
                     /protein_id="HCY0281696.1"
                     /translation="RFKKKVKPEVDMTIKEFILLTYLFHQQENTLPFKKIVSDLCYKQ
                     SDLVQHIKVLVKHSYISKVRSKIDERNTYISISEEQREKIAERVTLFDQIIKQFNLAD
                     QSESQMIPKDSKEFLNLMMYTMYFKNIIKKHLTLSFVEFTILAIITSQNKNIVLLKDL
                     IETIHHKYPQTVRALNNLKKQGYLIKERSTEDERKILIHMDDAQQDHAEQLLAQVNQL
                     LADKDHLHLVFE"
BASE COUNT          375 a          155 c          118 g          330 t
ORIGIN      
        1 tcgttttaag aaaaaagtca agcctgaagt cgatatgact ataaaagaat ttatattact
       61 gacttattta tttcatcaac aagaaaacac acttccattt aagaagattg tttcagattt
      121 atgttataaa caatcggatt tagtacagca tataaaagta cttgtgaaac attcatatat
      181 tagtaaagtt cgaagtaaaa ttgatgagcg taatacttac atttcaatat ctgaagaaca
      241 acgagagaaa attgcagaac gtgttacatt gtttgatcaa atcattaaac aatttaacct
      301 tgcagatcaa agtgaatcac agatgatacc aaaagatagt aaagaatttc taaacttgat
      361 gatgtataca atgtatttca agaatattat caaaaaacat ctaacattaa gttttgtaga
      421 attcacaatt ctagctatta tcacttctca aaataaaaac atcgttcttc ttaaagattt
      481 aattgaaaca atccaccata aataccctca aactgttaga gctctcaata atttaaaaaa
      541 gcaaggctat ctaataaaag aacgctcaac tgaagatgaa agaaaaattt taattcatat
      601 ggatgacgcg cagcaagacc atgctgaaca attattagct caagtgaatc aattattagc
      661 agataaagat catttacatc ttgtttttga ataatatctc tattacgcaa gtgtgctgta
      721 ttctaaagtg cacttgtgtt ttctattttt taataaaacc tcagcacata atgaacaact
      781 ttctattttc tatatcactt aaaaccattt ccgaaattaa acctcagcac attcaaagcc
      841 ccactttatt cttaaaaata ttttttaact catatgtatt aaaccgcttt cattataaaa
      901 aatatctcta tattttatct gtttttatta atcgaaatag cgtgattttg cggttttaag
      961 ccttttactt cctgaata
//
LOCUS       DAKTIO010000346          960 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.358, whole genome shotgun sequence.
ACCESSION   DAKTIO010000346 DAKTIO010000000
VERSION     DAKTIO010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.358"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            56..367
                     /locus_tag="O0293_002893"
     CDS             56..367
                     /locus_tag="O0293_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCY0281697.1"
                     /translation="MFKFLKKTQAFTLIEMLLVLLIISLLLILIIPNIAKQTAHIQST
                     GCNAQVKMVNSQIEAYALKHNRNPSSIEDLIADGFIKEAQKTCKSGETITISNGEAVA
                     N"
     gene            345..791
                     /locus_tag="O0293_002894"
     CDS             345..791
                     /locus_tag="O0293_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HCY0281698.1"
                     /translation="MEKQLQIRKQSAFTMIEMLVVMMLISIFLLLTMTSKGLSNLRVI
                     DDEANIISFITELNYIKSQAIANQGYINVRFYENSDTIKVIENNNIRFLKLKVGKIIN
                     VAKVDIIAFDKKGNINKFGSITIYNNNSIYRIIFHIEKGRIRYEKL"
     gene            778..>960
                     /locus_tag="O0293_002895"
     CDS             778..>960
                     /locus_tag="O0293_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGE"
                     /protein_id="HCY0281699.1"
                     /translation="MKSYKCKGSFLIDSMAGFLLIGLITLLLIPMMNQMQASINHKLQ
                     TIDASKVILTTVSKINK"
BASE COUNT          389 a          108 c          152 g          311 t
ORIGIN      
        1 attatgttac caatgtttca aatgatgcaa agtataaaat aatggaggtt aatatatgtt
       61 taaatttctt aagaaaactc aagcgtttac attgatagag atgctattag tgttattaat
      121 catcagttta ttattaattt taatcattcc aaatattgct aaacaaactg ctcacataca
      181 atcaacaggt tgtaatgcac aggtaaaaat ggttaatagt caaattgaag cgtatgcatt
      241 gaaacataat agaaatccat cgtctattga agacttaatt gcagatggtt ttataaaaga
      301 agcacaaaag acatgtaaat caggagagac aatcacaatt agtaatggag aagcagttgc
      361 aaattagaaa gcagtcagca tttactatga ttgagatgct tgtggtaatg atgttaatca
      421 gtatatttct acttttgaca atgacatcta aaggattaag caatcttaga gtaatagatg
      481 atgaggcaaa tatcatttct tttattactg aattgaatta tattaagtcg caagctatag
      541 caaatcaagg atatatcaat gttagatttt atgaaaacag tgacactatt aaagtaatag
      601 agaataataa tatacgattt ctaaaattaa aagtaggcaa aataattaat gttgcaaaag
      661 ttgatattat tgcctttgat aaaaaaggga atatcaataa atttggtagc ataacaattt
      721 acaataacaa ttcaatttat agaataatat tccatattga aaaaggaaga attcgttatg
      781 aaaagctata agtgtaaagg ttcattctta atagatagta tggctggatt tttgctaatt
      841 ggattgatta cattactatt gataccaatg atgaatcaaa tgcaagcgag tataaaccat
      901 aaactacaaa caattgatgc ttctaaagta attttgacga ctgtatctaa aattaataaa
//
LOCUS       DAKTIO010000347          937 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.360, whole genome shotgun sequence.
ACCESSION   DAKTIO010000347 DAKTIO010000000
VERSION     DAKTIO010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.360"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            57..>937
                     /locus_tag="O0293_002896"
     CDS             57..>937
                     /locus_tag="O0293_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HCY0281700.1"
                     /translation="MKKKLGMLLLVPAVTLSLAACGNDDGKDKDGKVTIKTTVYPLQS
                     FAEQIGGKHVKVSSIYPAGTDLHSYEPTQKDILSASKSDLFMYTGDNLDPVAKKVAST
                     IKDKDKKLSLEDKLDKAKLLTDQHEHGEEHEHEGHDHEKEEHHHHHGGYDPHVWLDPK
                     INQTFAKEIKDELVKKDPKHKDDYEKNYKKLNDDLKKIDNDMKQVTKDKQGNAVFISH
                     ESIGYLADCYGFVQKGIQNMNAEDPSQKELTKIVKEIRDSNAKYILYEDNVANKVTET
                     IRKETDAKPLKFYNMES"
BASE COUNT          388 a          126 c          176 g          247 t
ORIGIN      
        1 aagtagcatg agtatataat tttaaaacgt aaagattact atttaggagg gtgactatga
       61 aaaagaaatt aggtatgtta cttcttgtac cagccgtaac tttatcatta gccgcatgtg
      121 ggaatgatga tggaaaagat aaagatggca aggtaacaat taaaacgaca gtttatccat
      181 tgcaatcatt tgcagagcaa attggtggaa aacacgtgaa ggtatcatca atctatccag
      241 cagggacaga tttacatagc tatgaaccaa cacaaaaaga tatattaagt gcaagcaagt
      301 cagacttgtt tatgtataca ggggataatt tagatccggt tgctaagaaa gttgcatcta
      361 ctatcaaaga taaagataaa aaactgtctt tagaggataa attagataaa gcaaagcttt
      421 taactgatca acacgagcat ggtgaagagc atgaacatga gggacatgat catgagaaag
      481 aagaacatca tcatcatcat ggtggatatg atccacacgt atggttagat cctaaaatta
      541 accaaacttt cgctaaagaa attaaagatg aattagtgaa aaaagatcca aaacataaag
      601 atgactatga gaaaaactac aaaaaattaa acgacgatct taagaaaatt gataacgata
      661 tgaagcaagt tacaaaagat aagcaaggta atgcagtatt catttcacat gaatcaattg
      721 gatacttagc tgattgttat ggttttgttc aaaaaggtat tcaaaacatg aatgctgaag
      781 atccatcaca aaaagaatta actaaaattg ttaaagaaat tagagatagc aatgctaaat
      841 atattcttta cgaagataat gttgcgaata aagtgactga aacaattcgt aaagaaacag
      901 atgcgaagcc tttaaaattc tacaacatgg agtcttt
//
LOCUS       DAKTIO010000348          928 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.361, whole genome shotgun sequence.
ACCESSION   DAKTIO010000348 DAKTIO010000000
VERSION     DAKTIO010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.361"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            40..855
                     /gene="proC"
                     /locus_tag="O0293_002897"
     CDS             40..855
                     /gene="proC"
                     /locus_tag="O0293_002897"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCY0281701.1"
                     /translation="MKLVFYGAGNMAQAIFTGIINSSNLDANDIYLTNKSNEQALKAF
                     AEKLGVNYSYDDATLLKDADYVFLGTKPHDFDALATRIKPHITKDNCFISIMAGIPID
                     YIKQQLECQNPVARIMPNTNAQVGHSVTGISFSNNFDPKSKDEINDLVKAFGSVIEVS
                     EDHLHQVTAITGSGPAFLYHVFEQYVKAGTKLGLEKEQVEESIRNLIIGTSKMIERSD
                     LSMAQLRKNITSKGGTTQAGLDTLSQYDLVSIFEDCLNAAVDRSIELSNIEDQ"
BASE COUNT          339 a          155 c          155 g          279 t
ORIGIN      
        1 tataacatac aaatcaaaaa ctaaagggat gtgacgttaa tgaaactcgt attttatgga
       61 gctggtaata tggcacaagc tatatttaca ggaattatta actcaagcaa cttagatgcc
      121 aatgatatat atttaacaaa taaatctaat gaacaagctt taaaagcatt cgctgaaaaa
      181 ctaggtgtta actatagtta tgatgatgcg acattattaa aagatgcaga ttatgtattt
      241 ttaggtacca aaccacatga ttttgatgct ctagcaacac gcatcaaacc acatattaca
      301 aaagacaatt gcttcatttc aattatggca ggtattccga ttgattatat taaacaacaa
      361 ttagaatgcc aaaatccagt tgctagaatt atgccaaaca caaatgcgca agttggacac
      421 tctgttactg gcattagttt ttcaaacaac tttgacccta aatctaaaga tgaaattaac
      481 gatttagtta aagcatttgg ttctgtaatt gaagtatcag aagatcattt acatcaagta
      541 acagctatca ccggaagcgg cccagcattt ttatatcatg tattcgagca atatgttaaa
      601 gctggtacga aacttggtct agaaaaagaa caagttgaag aatctatacg caaccttatt
      661 ataggtacaa gtaagatgat tgaacgttca gatttgagca tggctcaatt aagaaaaaat
      721 attacctcta aaggtggtac gacacaagct ggccttgata cattgtcaca atatgattta
      781 gtatctattt tcgaagattg tctaaacgct gccgtcgacc gtagtattga actttctaat
      841 atagaagacc aataaaaaca aacccgccaa cacatgtatg catcatcgca agcactgtgt
      901 ttgacgggtt atttttataa tttattgt
//
LOCUS       DAKTIO010000349          928 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.362, whole genome shotgun sequence.
ACCESSION   DAKTIO010000349 DAKTIO010000000
VERSION     DAKTIO010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.362"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..615)
                     /locus_tag="O0293_002898"
     CDS             complement(<1..615)
                     /locus_tag="O0293_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HCY0281702.1"
                     /translation="MNTIYEPSSICMIRTPLLSVEFFNLFLNTEQIKYSDLQLNAQMK
                     ESILTTTFNLYCTLQEINFDGDNKKVRDAKESLLKYLIRMSTRPTPFGLLSGINLGHF
                     VNEPTRLKVGNSIQKYVKVDGEWLYKLVSYIESIDEYYQNLKVIWNSKAHIINDRIYL
                     NEQSAIYLNNNKDTSFSIKNSELLVFIKTTVTNNNITFSNLAEKI"
     gene            complement(680..823)
                     /locus_tag="O0293_002899"
     CDS             complement(680..823)
                     /locus_tag="O0293_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="HCY0281703.1"
                     /translation="MEKVLDLDVQVKANNNSNDSAGDERITSHSLCTPGCAKTGSFNS
                     FCC"
BASE COUNT          313 a          149 c          113 g          353 t
ORIGIN      
        1 tatcttttca gctaaatttg aaaaagtaat attattattc gtcacagtgg ttttgatgaa
       61 tacgagtaat tcgctatttt taatggaaaa agatgtatct ttattgttgt ttaaataaat
      121 agcactttgt tcatttaaat atattcgatc attgataata tgtgctttac tattccaaat
      181 aactttcaaa ttttgataat attcatcaat actctcaata taactaacca gtttatacaa
      241 ccattcgcca tcgactttca catatttttg aatactattt ccaactttaa gacgtgttgg
      301 ttcatttaca aaatgcccca aattaatacc acttaataat ccaaatggag ttggacgtgt
      361 tgacattcta attaaatatt ttaataaact ttctttagca tctctaacct tcttgttatc
      421 gccatcaaaa tttatttctt gtaaagtaca atataagtta aatgttgttg ttaggattga
      481 ttctttcatc tgagcattta attgtaagtc gctatattta atttgttcag tatttaaaaa
      541 taaattgaaa aattcaactg ataataaagg tgttctaatc atacaaatac ttgacggttc
      601 gtaaatagtg ttcatttgta agcacctcac atatttgaat agacaaatag agccctttca
      661 ggctctattt gctgtaatct taacagcaga agctattaaa actaccagtc ttagcacaac
      721 caggagtaca taaactatga cttgtaatac gttcgtcacc tgctgaatca tttgagttat
      781 tgtttgcttt aacttgcacg tctaaatcaa gaactttttc catagataaa acctcctatt
      841 attagttgaa gttaaggcct acttcaattg tcatattatc ccttttcaca caacaaatca
      901 ataaattata taattatatt ttgtgaat
//
LOCUS       DAKTIO010000350          906 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.363, whole genome shotgun sequence.
ACCESSION   DAKTIO010000350 DAKTIO010000000
VERSION     DAKTIO010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.363"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..311
                     /locus_tag="O0293_002900"
     CDS             <1..311
                     /locus_tag="O0293_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HCY0281704.1"
                     /translation="KYYEPLIHKNDKEFKEGMQRARKELNYTANTNTVATLFSTNDER
                     NRKEKINNVIDLSEKIERTKDMPIKNTITTQLGNKLIGTKKARFDDKKVVSFGAFEDE
                     "
     gene            304..510
                     /locus_tag="O0293_002901"
     CDS             304..510
                     /locus_tag="O0293_002901"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281705.1"
                     /translation="MNKINDRDLTELSSYRVYQDINKDNDFTVNEKRFKQADVFEDLY
                     REKLKDTNKLREYNYLQNETFKSA"
     gene            525..>906
                     /locus_tag="O0293_002902"
     CDS             525..>906
                     /locus_tag="O0293_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HCY0281706.1"
                     /translation="MLKKAKFILMATILLSGCSTTNNESNKETKSVPEEMDASKYVGQ
                     GFQPPAEKDAIEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVIGSGDGVEVFVHCDDH
                     DIVFNASIPFDKSIIDSDSSLRSKD"
BASE COUNT          393 a          108 c          169 g          236 t
ORIGIN      
        1 gaaaatatta cgaaccattg atacataaaa atgataaaga gtttaaagaa ggtatgcaac
       61 gagctagaaa agagctaaac tatactgcta atacaaatac tgtagcaacg ttgtttagta
      121 cgaatgatga aaggaataga aaagaaaaga taaataatgt aatagattta tccgagaaaa
      181 ttgaaagaac aaaagatatg ccaatcaaga atactataac tactcaatta ggaaataaac
      241 ttattggcac aaaaaaagct cgttttgatg ataagaaagt agtgtcgttt ggagcatttg
      301 aagatgaata aaataaatga tagagattta acagaattga gtagctatag ggtttatcaa
      361 gacatcaata aagataatga ctttacagtt aacgaaaaac gatttaagca ggcagatgta
      421 tttgaagatt tatatagaga gaaactaaaa gacacaaata aattaagaga gtataattat
      481 ttacaaaatg aaacttttaa aagcgcataa ataggtgatg agatatgctt aaaaaagcaa
      541 aatttatctt aatggcaacg atactactat caggatgttc aactaccaat aacgaatcca
      601 acaaagaaac aaaatctgta ccagaagaaa tggatgcttc aaaatatgta ggacaaggat
      661 tccaaccacc tgcagaaaaa gatgcgattg aatttgcaaa gaagcataaa gataaaattg
      721 ctaagcgagg cgaacaattt tttatggata acttcggtct aaaagttaaa gctacaaatg
      781 ttataggtag tggcgatggt gtagaagtat tcgtgcattg tgatgaccac gacatcgtat
      841 ttaatgcgag tattccattt gataaatcaa ttattgacag tgatagctca ttaagaagta
      901 aggata
//
LOCUS       DAKTIO010000351          897 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.364, whole genome shotgun sequence.
ACCESSION   DAKTIO010000351 DAKTIO010000000
VERSION     DAKTIO010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.364"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(31..684)
                     /locus_tag="O0293_002903"
     CDS             complement(31..684)
                     /locus_tag="O0293_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HCY0281707.1"
                     /translation="MELKVDDFVKNIKRPYLTVLGVFVVAVSLFFDAIMFFYAKLYDK
                     LPMYLLVFMAFTAVILIMMYIQEKNENYKVEKRYVVRYLTLNVIVGYTLPLLFVSIYV
                     FGVVGFGFDVFNYCLGIILMLFISWLGLFLFYKNEFDSENPNPAVNAIAIIIKLFAFG
                     GIFYISLIVPVTQQEEIFIGLSIFINIIVDALLVRSYFNYALYKSIKKDIENEGKTM"
BASE COUNT          359 a          154 c           76 g          308 t
ORIGIN      
        1 tagactccta ttatttttta tgtctttcct ttacattgtc ttaccttcat tttcgatatc
       61 tttcttaata ctcttatata acgcgtaatt aaaatatgac ctaacaagaa gagcatcaac
      121 aattatattg atgaatatac ttaaccctat aaaaatctct tcttgctgtg ttacaggaac
      181 tattagactt atataaaata ttcctccaaa agcaaataat tttataataa ttgctatggc
      241 attaactgca ggattaggat tttcactatc aaattcgttt ttataaaata aaaataaacc
      301 taaccaagaa ataaataaca tcaagataat acctagacaa taattgaaaa catcaaatcc
      361 aaaaccgact acaccaaaaa cgtaaataga tacaaaaagc aatggcaaag tatatcccac
      421 aataacgtta agtgtgagat atctaaccac atatcttttt tcaactttgt aattttcatt
      481 tttctcttgt atgtacatca taatcaaaat tacagctgta aaggccataa acaccagtaa
      541 atacataggc aatttatcat atagtttagc gtagaaaaac ataatcgcgt caaaaaataa
      601 tgaaactgca actacaaata ctcccaatac agtcaagtat ggtcttttta tattctttac
      661 aaaatcatcg actttcaatt ccataaattc ataatctccc caatcttact tcaatatttt
      721 tataagttaa ataaatcttc ttcgccaaaa aaatgcatat tattaagtta accaacattt
      781 ttcaagaatc attatgtaat caaactattt tttgtagttt attagatttc caataactta
      841 ttaaacatta ttataactaa aattctatta atttaaacaa aatttaaaat aaaccat
//
LOCUS       DAKTIO010000352          873 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.366, whole genome shotgun sequence.
ACCESSION   DAKTIO010000352 DAKTIO010000000
VERSION     DAKTIO010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.366"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            33..860
                     /locus_tag="O0293_002904"
     CDS             33..860
                     /locus_tag="O0293_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0281708.1"
                     /translation="MWKWEAENDAKGVVVIAHNILEHTGRYAYVITMLRRNGYHVIMG
                     DLPGQGQTSRANKGQIENFQTYHESLLDWLKIANEYKIPTYVLGVGLGGLILLNLLEK
                     VELPIEGMMLISPMLELQKNGKNRKDKLVSNIGKISKDTRFNVGVEPKDLTRNLEIVE
                     ETVNDGLMLKKATYHWYNTINETMKDTMAHIHDIQPMPTLLMYGTKDLIVDTRAIDEF
                     KEKYQTPELYFKAWQGFYHEVHNEPERDEVMRYILTFLNNSVNTMGFIVEDDEIVEI"
BASE COUNT          330 a          102 c          179 g          262 t
ORIGIN      
        1 ttgaagatta atgaaaaagg ggcagtataa taatgtggaa gtgggaagct gaaaatgatg
       61 cgaaaggcgt cgttgttatt gctcataata tattagaaca tacgggaaga tatgcatatg
      121 ttattacgat gttaagaaga aatggctatc atgtgattat gggagatttg cctggtcaag
      181 gtcaaacttc aagagctaat aaaggacaaa ttgaaaactt tcaaacgtat cacgaaagtt
      241 tgctagactg gttaaagata gctaatgaat ataaaattcc tacttatgta ttaggggtag
      301 gacttggcgg tttgatttta ttaaatttac ttgaaaaagt agaattgcca atcgagggga
      361 tgatgttaat ttctcctatg ctagaacttc aaaagaatgg aaaaaatcgt aaagataaac
      421 ttgtttcaaa tataggtaaa atttcaaaag acacacgttt taatgttggt gtagaaccaa
      481 aagatttaac acgtaattta gaaattgttg aagaaacagt taacgatggt ttaatgttaa
      541 aaaaagcaac gtatcattgg tataatacaa ttaatgagac gatgaaagat acgatggctc
      601 atattcatga cattcaacca atgccaacgc tattaatgta tggaacgaag gatttaattg
      661 ttgatactag ggcaattgat gagtttaaag aaaaatatca aacacctgaa ttatatttta
      721 aagcttggca aggtttttac cacgaggttc acaatgagcc agaacgtgat gaagtaatgc
      781 gttatattct gactttctta aataacagcg tcaatacaat gggctttatt gttgaagatg
      841 acgaaattgt agaaatttaa tattctaaaa agg
//
LOCUS       DAKTIO010000353          871 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.367, whole genome shotgun sequence.
ACCESSION   DAKTIO010000353 DAKTIO010000000
VERSION     DAKTIO010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.367"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            377..751
                     /locus_tag="O0293_002905"
     CDS             377..751
                     /locus_tag="O0293_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HCY0281709.1"
                     /translation="MAITKINDCFELLSMVTYADKLKSLIKKEFSISFEEFAVLTYIS
                     ENKEKEYYLKDIINHLNYKQPQVVKAVKILSQEDYFDKKRNEHDERTVLILVNAQQRK
                     KIESLLSRVNKRITEANNEIEL"
BASE COUNT          349 a          111 c          115 g          296 t
ORIGIN      
        1 aagaatctct ttagacacac attgaatatc gaaacattta attgcgctaa atcgtttcat
       61 taaataaatt accttgtatt gtcgattaaa ttaaggtaaa ttataaaaaa tgctgatatt
      121 tttgactaaa ccaaatgcta acccagaaat acaatcactg tgtctaatga ataatttgtt
      181 ttataaacac ttttttgttt acttctcatt tttaattagt tataattaac taaataatag
      241 agcattaaat atatttaata aaacttattt aatgcaaaat tatgactaac atatctataa
      301 taaataaaga ttagatatca atatattatc gggcaaatgt atcgagcaag atgcatcaaa
      361 tagggaggtt ttaaacatgg caattacaaa aatcaatgat tgctttgagt tgttatcaat
      421 ggtcacttat gctgacaaat taaaaagttt aattaaaaag gaattttcaa ttagctttga
      481 agaattcgct gtattgacat acatcagcga aaacaaagag aaagaatact atcttaaaga
      541 tattattaat catttaaact acaaacaacc acaagttgtt aaagcagtta aaattttatc
      601 tcaagaagat tacttcgata aaaaacgtaa tgagcatgat gaaagaactg tattaattct
      661 tgttaatgca caacaacgta aaaaaatcga atcattattg agtcgagtaa ataaacgaat
      721 cactgaagca aacaacgaaa ttgaactata attttgttta gcgcaatttg gtgaagtttg
      781 atagatgata cattctatta aacttccttt ttttatgctc tttttaccta attgttaaga
      841 ggttttgcac taatggcact taatataagg a
//
LOCUS       DAKTIO010000354          866 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.368, whole genome shotgun sequence.
ACCESSION   DAKTIO010000354 DAKTIO010000000
VERSION     DAKTIO010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.368"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..187
                     /locus_tag="O0293_002906"
     CDS             <1..187
                     /locus_tag="O0293_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0281710.1"
                     /translation="SEGKLKVSFDYIDWINTEFDQLGRENYYMYKKFGVLPEMEYEME
                     EIKEIDQYIKEQDEAEI"
     gene            198..686
                     /locus_tag="O0293_002907"
     CDS             198..686
                     /locus_tag="O0293_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin YezG family protein"
                     /protein_id="HCY0281711.1"
                     /translation="MTFEEKLSQMYNEIANEISGMIPVEWEKVYTIAYVDDEGGEVVF
                     NYTKPGSEDLNYYSDIPKDCNVSKDIFKNSWFKVYRMFDELRETFKKEDLEPWTSCEF
                     DFTRKGNLKVSFDYIDWIKLGFGPSGKENYYMYKKFGILPDMEYEMEEIRAVEKYVKE
                     QE"
BASE COUNT          358 a           83 c          167 g          258 t
ORIGIN      
        1 aagcgaaggt aaattaaaag tttcatttga ttatatagat tggataaata cagagtttga
       61 tcaattaggc cgtgaaaatt attatatgta taaaaaattt ggggttttac cagaaatgga
      121 atatgaaatg gaagaaatta aagaaatcga tcaatatatt aaagagcaag atgaagctga
      181 aatatagggg agataacatg actttcgaag aaaagctaag tcaaatgtac aatgaaattg
      241 caaatgaaat cagtggaatg ataccagtag aatgggaaaa agtatataca attgcctacg
      301 tagatgatga aggtggagag gttgttttta attatactaa accaggaagt gaagatttga
      361 attattattc agatattcct aaagattgca atgtctcaaa agatattttt aagaattcat
      421 ggtttaaagt ttatcgaatg tttgatgagt taagagaaac ttttaaaaaa gaagatttag
      481 aaccgtggac atcatgtgaa tttgacttta caagaaaggg aaatttaaaa gtatcatttg
      541 attatataga ttggattaaa ttaggttttg gcccatcagg aaaggaaaac tactatatgt
      601 acaaaaaatt tggtatttta ccagatatgg aatatgaaat ggaagaaatt cgagcagtag
      661 agaagtatgt taaagagcaa gagtagcaga catgttataa aagactgtgc aaaatcaccc
      721 tcgttttaca tttgattcaa agaagaaggt aaaagataag attatttgca acttaaaaag
      781 tcaattagct tatcggtatc catacatcat ggataaatga gttcaactaa ttaacaaatc
      841 acgatataaa tttaaaattg tcatta
//
LOCUS       DAKTIO010000355          858 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.369, whole genome shotgun sequence.
ACCESSION   DAKTIO010000355 DAKTIO010000000
VERSION     DAKTIO010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.369"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..616
                     /gene="icaB"
                     /locus_tag="O0293_002908"
     CDS             <1..616
                     /gene="icaB"
                     /locus_tag="O0293_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="intercellular adhesin biosynthesis
                     polysaccharide N-deacetylase"
                     /protein_id="HCY0281712.1"
                     /translation="KWLKSHDAKFLTLKEFLYYKKKGKFPKRSVWINFDDMDETIYEN
                     AYPILKKYKIPATGFIITGHVGEENFHNLDMISKKELKEMYKTGLWEFETHTHDLHNL
                     SKNNKSKLMKASEATIIKDLNKSEKYLTKNFKKSQKTIAYPYGLMNDDKLPVIKKAGL
                     KYGFSLEEKAVTPNSNDYYIPRILISDDAFEHLIKRWDGFHEKD"
     gene            603..>858
                     /locus_tag="O0293_002909"
     CDS             603..>858
                     /locus_tag="O0293_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine export
                     protein"
                     /protein_id="HCY0281713.1"
                     /translation="MKKIRLELVYLRAIICAIIIITHLLTQITLKHENMEGGSLVLQF
                     YIRNIVIFGTPCFIILSQLLTTLNYQKVTYRYLTTRVKYIL"
BASE COUNT          339 a          123 c          130 g          266 t
ORIGIN      
        1 aaaatggcta aaatcacatg atgctaaatt tttaaccttg aaagaatttt tatattacaa
       61 gaaaaaaggt aagtttccaa aacgaagtgt atggattaac tttgatgata tggatgaaac
      121 tatttatgaa aatgcttatc caatcttaaa aaaatataaa ataccggcaa ctgggtttat
      181 tatcacaggt catgttgggg aagaaaactt tcacaacctc gatatgatta gtaaaaaaga
      241 actaaaagaa atgtataaaa ctgggttatg ggaatttgaa acacataccc acgatttgca
      301 taacttatct aaaaataata agtcaaaatt aatgaaagct tctgaagcta caatcataaa
      361 agatttaaac aaaagtgaaa aatatctaac taaaaacttt aaaaagtcgc agaaaactat
      421 agcctatcct tatggcttga tgaatgacga taaattaccg gtaatcaaaa aagctgggtt
      481 aaaatacggt ttttcattag aggaaaaagc agtcactccg aactccaatg attattacat
      541 ccctagaata ttaattagtg atgatgcttt tgagcattta attaagagat gggacggatt
      601 ccatgaaaaa gattagactt gaactcgtat atttacgtgc tattatatgt gcaattatta
      661 ttatcacaca tttacttaca caaattactt taaaacatga aaatatggag ggtggatcct
      721 tagtgttaca attttacatt cgtaatattg tgatttttgg tacaccttgc tttattatct
      781 tgtcacagtt actgacaacc ttgaattacc aaaaagtcac ctatagatac ttaactacac
      841 gcgtaaaata tatactta
//
LOCUS       DAKTIO010000356          826 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.372, whole genome shotgun sequence.
ACCESSION   DAKTIO010000356 DAKTIO010000000
VERSION     DAKTIO010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.372"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            213..368
                     /locus_tag="O0293_002910"
     CDS             213..368
                     /locus_tag="O0293_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281714.1"
                     /translation="MMVKKLLKAIKQNFLYEQSNITFKMIVFGYKRLSKMLFQFEINH
                     INFLWEG"
     gene            376..816
                     /locus_tag="O0293_002911"
                     /pseudo
     CDS             376..816
                     /locus_tag="O0293_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499366.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          314 a           80 c          146 g          286 t
ORIGIN      
        1 tgtaatatat cgaaagattt aaatgtaaaa ttttgatttg ttaagaaatt acgtttgtaa
       61 aaataaaaaa atcaacttat ttgtatgaga taaatatgta ttgaagaaga cgtgttatta
      121 atttggaaaa tactggtcaa agatgggagc tcttaaaagc gttattgtat tttttagtca
      181 atacaaatag attgccgtaa taataatcgt acttgatggt taaaaaatta cttaaggcta
      241 taaagcaaaa ctttttatat gagcagtcga atataacgtt taaaatgatt gtttttggat
      301 ataaacgatt aagtaaaatg ctttttcagt ttgaaattaa tcatataaat ttcttatggg
      361 agggttgata tcttaatgat taacattatt tcagctatag gatctattgg aacatttatt
      421 atggctttat tttattttgt atcagtttca gttcaacttt atcaaatgaa aattagcttt
      481 ctgccagctt taggttttaa ccaaatttta ttagaaaggg aggaggatca acttaatata
      541 atgaattcgg caacagaaga gcatcatcat aaagattata ttaaactata taatttaggt
      601 ggcggtgctg ctaaaaaaat tgcaatagag gttttattgg ggaaggataa agtcattcag
      661 aaaaaatacg tgcatatttt acctagtaaa gaagggtaca tgttaccaat taataaaaat
      721 gtgtacgaag aattagaaag aacgattgag aacaatggtc atgaagctga tttgaatgta
      781 cgtatgactt attatcataa tgtaagtcgc aaacaatagg aagtta
//
LOCUS       DAKTIO010000357          819 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.373, whole genome shotgun sequence.
ACCESSION   DAKTIO010000357 DAKTIO010000000
VERSION     DAKTIO010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.373"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
BASE COUNT          199 a          196 c          126 g          298 t
ORIGIN      
        1 ttcgatttaa aagatattag acataaaatc taaaaacagc agtaagatga tttatgatta
       61 aaaactatct tactgctgtt cactttttat aatacttctg aatgtcttca cttatacttc
      121 tagtcacaga tttaaataat caaaagtgca cattattaaa atatcaattt cacactcaat
      181 gcggctcatc gcattcattt cttgtctagc aacgttctac tctagcggaa cgtaagttag
      241 ctaccatcct cgctaagaac ctttcttgac ttgtgacaat cgcttgcttc tttcctctcc
      301 ttcggctctc gcttactcat ttagctctac taaactcgtt gcgctctttt ctcgtttcgt
      361 cagattcaaa cgttttcact tcgccaagcc atttttcttt gtgtttactt tttattttga
      421 cgttttagac ataaaaaaag agacctcacg gtctcaactt gcctggcaac gttctactct
      481 agcggaacgt aagttggcta ccatcgtcgc taaagacctt tcttgacttg tgacaatcgc
      541 ttgcttcttt cctcttcttc ggctctcgct tactcattta gctctactaa actcgttgcg
      601 ctcttttctc gtttcgtcag attcaaacgt tttcacttcg ccaagccatt tttctttgtg
      661 tttgcttttt attttgacgt tttagacata aaaaaagaga ccttgcggtc tcaatgcggc
      721 tcatcgcatc cattttttgc ctggcaacgt tctactctag cggaacgtaa gttcgactac
      781 catcgacgct aaggagctta acttctgtgt tcggcatgg
//
LOCUS       DAKTIO010000358          807 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.374, whole genome shotgun sequence.
ACCESSION   DAKTIO010000358 DAKTIO010000000
VERSION     DAKTIO010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.374"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(89..469)
                     /gene="gcvH"
                     /locus_tag="O0293_002912"
     CDS             complement(89..469)
                     /gene="gcvH"
                     /locus_tag="O0293_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCY0281715.1"
                     /translation="MAVPNELKYSKEHEWVKVEGNVAIIGITEYAQSELGDIVFVELP
                     ETDDEINEGDTFGSVESVKTVSELYAPISGKVVEVNEELEDSPEFVNESPYEKAWMVK
                     VEISDESQIEALLTAEKYSEMIGE"
     gene            complement(627..>807)
                     /locus_tag="O0293_002913"
     CDS             complement(627..>807)
                     /locus_tag="O0293_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HCY0281716.1"
                     /translation="HGAKYRELDLKNKLQTLSDDEKLELLSSDGMLVKRPLAVMGDKI
                     TLGFKEDQYKETWLA"
BASE COUNT          241 a          166 c           88 g          312 t
ORIGIN      
        1 tagtacctaa taatttttct ttcatatcat ttattttctc agactaaaaa taaactcctc
       61 aatcttagat taaggagtta cacggtgatt attcaccaat catttctgaa tatttttcag
      121 ctgttaataa agcttcaatc tgactttcat cactaatttc tacttttacc atccatgctt
      181 tttcgtatgg agattcattt acaaattcgg gactatcttc tagttcttcg ttgacttcaa
      241 ctactttacc agagattggt gcatataatt ctgatacagt tttaactgat tctacgctac
      301 caaacgtatc cccttcatta atttcatcat ctgtttctgg taattcaacg aaaacaatat
      361 cacctaactc gctttgtgcg tattctgtga ttccaattat tgctacatta ccttcaactt
      421 taacccattc atgctctttt gaatatttca attcatttgg tactgccaag agaatcccct
      481 cctaattaat ctttacatca ttaatgatgc cataaggcta gatgctattc aactatattt
      541 ataaaaatta acaattgtat agcttatttc attttacatg taatgaatct aaatatcgag
      601 atatttagta aacgcttaca tttcacttac gctaaccaag tctctttata ttgatcttct
      661 ttaaatccta atgttatctt atcgcccatt actgctagag gacgctttac taacatacca
      721 tcagatgata acaactctaa cttttcatca tctgataaag tttgtaattt atttttcaaa
      781 tcaagctcac gatatttcgc gccgtgt
//
LOCUS       DAKTIO010000359          800 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.375, whole genome shotgun sequence.
ACCESSION   DAKTIO010000359 DAKTIO010000000
VERSION     DAKTIO010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.375"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            57..656
                     /locus_tag="O0293_002914"
     CDS             57..656
                     /locus_tag="O0293_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCY0281717.1"
                     /translation="MAFKLPNLPYAYDALEPYIDQRTMEFHHDKHHNTYVTKLNATVE
                     GTELEHQSLADMIANLDKVPEAMRMSVRNNGGGHFNHSLFWEILSPNSEEKGGVIDDI
                     KAQWGTLDEFKNEFANKATTLFGSGWTWLVVNDGKLEIVTTPNQDNPLTEGKTPILLF
                     DVWEHAYYLKYQNKRPDYMTAFWNIVNWKKVDELYQAAK"
BASE COUNT          305 a          121 c          146 g          228 t
ORIGIN      
        1 aacacacccg aaattaatta ttataaaagt atattcataa aaggaggaat atacttatgg
       61 catttaaatt accaaattta ccatatgcat atgatgcatt ggaaccatat atagatcaaa
      121 gaacaatgga gtttcatcac gacaaacatc acaatacgta cgtgacgaaa ttaaacgcaa
      181 cagttgaagg aacagagtta gagcatcaat cactagcgga tatgattgct aacttagaca
      241 aggtaccgga agcgatgagg atgtcagtcc gtaataatgg cggtggtcat tttaaccatt
      301 cattattctg ggaaatacta tcacctaatt ctgaagaaaa aggtggcgta atagatgaca
      361 tcaaagcgca gtggggcact ttagatgaat ttaaaaatga atttgcaaat aaagcaacaa
      421 cattatttgg atcaggttgg acttggttag ttgttaatga tggcaaatta gaaattgtga
      481 caacgccaaa ccaagataat ccattaacag aaggcaaaac accaatctta ctatttgatg
      541 tttgggagca tgcctactat ctgaaatatc aaaataaacg tccagactat atgactgcat
      601 tttggaacat tgttaactgg aaaaaagttg atgaattata ccaagcagca aaataatata
      661 acttaatata attgaggtgg agcatctaca aggtgttcca cttttttgta cttaatatct
      721 ttacttattt gtttttgtgt aatgggatag cacgtaaaag tggaaaaata ataagataaa
      781 tttcaactcg aaatacctta
//
LOCUS       DAKTIO010000360          783 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.376, whole genome shotgun sequence.
ACCESSION   DAKTIO010000360 DAKTIO010000000
VERSION     DAKTIO010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.376"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            319..768
                     /locus_tag="O0293_002915"
     CDS             319..768
                     /locus_tag="O0293_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-inhibiting protein CHIPS"
                     /protein_id="HCY0281718.1"
                     /translation="MKKKLATTVLALSFLTAGISTHHHSAKAFTFEPFPTNEEIESNK
                     KMLEKEKAYKESFKNSGLPTTLGKLDERLRNYLKKGTKNSAQFEKMVILTENKGYYTV
                     YLNTPLAEDRKNVELLGKMYKTYFFKKGESKSSYVINGPGKTNEYAY"
BASE COUNT          334 a           94 c          106 g          249 t
ORIGIN      
        1 tatagcacaa aaaaactagc ccgaaggcta gcttatgaat agatgaaaat ttgaacacat
       61 tgctgtgtct aagataaata tagcacatat taattactat taatagaaaa attatagcgt
      121 tttttagatt atttcaattt atcgagacca aattcatcaa aacactaatt gaattttaat
      181 ttttagttaa aaataattaa tcttttatta atttcaagtt aattaatata tttcgtcgat
      241 tagattaaac tcattttagg aatttcgcaa aactaactat aaatttaaat atagaattta
      301 aggagaatta acatcattat gaaaaagaaa ttagcaacaa cagttttagc attaagtttt
      361 ttaacggcag gaatcagtac acaccatcat tcagcgaaag cttttacttt tgaaccgttt
      421 cctacaaatg aagaaataga atcaaataag aaaatgttag agaaagaaaa agcttataaa
      481 gaatcattta aaaatagtgg tcttcctaca acgctaggaa aattagatga acgtttgaga
      541 aattatttaa agaaaggcac aaaaaattct gctcaatttg aaaaaatggt tattttaact
      601 gaaaataaag gttactatac agtatatctg aatacaccac ttgctgaaga tagaaaaaat
      661 gttgagttac taggtaaaat gtataaaaca tacttcttta aaaaaggaga gtctaaatca
      721 tcttatgtaa ttaatggtcc tggaaaaact aatgaatatg catactaata gtagttacat
      781 aaa
//
LOCUS       DAKTIO010000361          775 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.377, whole genome shotgun sequence.
ACCESSION   DAKTIO010000361 DAKTIO010000000
VERSION     DAKTIO010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.377"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..113
                     /locus_tag="O0293_002916"
     CDS             <1..113
                     /locus_tag="O0293_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HCY0281719.1"
                     /translation="INNVTAFTANIYYKSIIKITITVKVGTNVQTKTIYV"
     gene            210..356
                     /locus_tag="O0293_002917"
     CDS             210..356
                     /locus_tag="O0293_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281720.1"
                     /translation="MMLTAIYFSIFTFYISQYSLKLKTLYNIETDYNYKIVLLLKEGK
                     THES"
     gene            346..>775
                     /locus_tag="O0293_002918"
     CDS             346..>775
                     /locus_tag="O0293_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HCY0281721.1"
                     /translation="MNHDKSPIILIGFMGTGKSTIGKYVADEQNLSFIDIDSYIEEKY
                     KLTIPEIFCKHGEQYFRNLEFTCLQECINTADIIATGGGIIESEEAFNFLKNQKNIIW
                     LDCNIDIIYSRINDDPHRPNANNKTIKQLNDLYCSRNLRYN"
BASE COUNT          300 a           86 c          120 g          269 t
ORIGIN      
        1 tgattaataa tgttacagca tttactgcaa atatctacta taaatccatt attaaaataa
       61 cgataacagt taaagtcggt acaaatgtgc agactaaaac tatttatgta taacttacta
      121 gtatttaaat gagtactatt aaaaaggatg tgaaataatt tattaatttt gatttatata
      181 aagaaaaagg atatgttacg ccgttcttat tgatgctaac agcaatttat ttttccattt
      241 tcacttttta tatcagtcaa tatagtttaa aattaaagac attatataat attgaaactg
      301 actataacta taagattgtt ttgttattga aagaagggaa aacgcatgaa tcatgataaa
      361 tcaccaataa ttttaattgg tttcatgggt accggtaaat ctacgattgg taaatacgtt
      421 gcagatgagc aaaatttatc atttattgat atagattcat atatcgaaga gaagtataag
      481 ttaacaatac cagaaatatt ttgtaaacat ggtgaacaat atttcaggaa tttagagttc
      541 acatgtttgc aagaatgtat taacactgca gatataattg ctactggtgg tggtattatt
      601 gagagtgaag aggcatttaa ttttttgaaa aatcaaaaaa acattatttg gttagattgt
      661 aatattgata ttatatatag tcgaatcaat gatgacccac atcgacctaa tgcaaataat
      721 aagacaatca agcagttaaa tgacttgtat tgctcgcgga atttaagata taatg
//
LOCUS       DAKTIO010000362          768 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.378, whole genome shotgun sequence.
ACCESSION   DAKTIO010000362 DAKTIO010000000
VERSION     DAKTIO010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.378"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            74..700
                     /locus_tag="O0293_002919"
     CDS             74..700
                     /locus_tag="O0293_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCY0281722.1"
                     /translation="MGLFKKDKKAMTFDNAMEERRSIYNLKDSISISDDELESMIAHA
                     VKHVPSSFNSQSTRIVLLLNDNNNKFWDNTKAILKEVMGENRDFEPTEQKIDNFKHSY
                     GTILFYEDQDVVSGLQEQMPNYYDNFAIWSTQTNAMHQFAIWTALATKGIGASLQHYN
                     PLVDEMTSNEFNIPKSWKLIAQMPFGDIREAAGEKTFNPVEDRFVIKK"
BASE COUNT          294 a          105 c          126 g          243 t
ORIGIN      
        1 tgctttacaa gtacgagtat aaattttata cttagtctat taagtattct ttactaaaga
       61 aggagcaata attatgggct tatttaaaaa agataaaaaa gcaatgactt ttgacaatgc
      121 aatggaagaa agacgttcaa tatataattt gaaagattcg atatcaatta gcgatgacga
      181 attagaatca atgattgcac atgcagttaa acatgttcct tcatcattta attctcaatc
      241 aactagaatt gttctcttgt taaatgacaa caataataaa ttttgggata acacaaaagc
      301 aattttaaaa gaagtcatgg gagagaatcg tgatttcgag ccaactgaac aaaaaattga
      361 taacttcaaa cattcatacg gtacaatttt gttttatgaa gatcaagatg ttgtaagtgg
      421 tttacaagaa caaatgccaa actactatga taattttgca atatggtcaa ctcaaacaaa
      481 tgccatgcat caatttgcaa tttggactgc attagctact aaaggcatag gtgcttcatt
      541 acaacactat aatccacttg ttgatgagat gacttcaaat gaatttaata ttcctaaaag
      601 ttggaaactt attgctcaaa tgccatttgg tgatattcgt gaagctgctg gcgaaaaaac
      661 atttaatcca gtggaagatc gtttcgtaat aaaaaaataa tcaatatatt gttaggaatg
      721 ggacagaaat gataaagaat cattagttat tcattatgta gtagttct
//
LOCUS       DAKTIO010000363          764 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.379, whole genome shotgun sequence.
ACCESSION   DAKTIO010000363 DAKTIO010000000
VERSION     DAKTIO010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.379"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(1..132)
                     /locus_tag="O0293_002920"
                     /pseudo
     CDS             complement(1..132)
                     /locus_tag="O0293_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458050.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(436..690)
                     /locus_tag="O0293_002921"
     CDS             complement(436..690)
                     /locus_tag="O0293_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281723.1"
                     /translation="MLIKIRGGNMKRDFFEKWSFILNLILFCAFLFLVVLMFYKDVDL
                     IYIFCTLLISLLPLLNIIKRIGWEINQSSMIYEVILAVID"
BASE COUNT          250 a          136 c          101 g          277 t
ORIGIN      
        1 ttgacgtaat ataatcccta tatctttttt tatttttcca tatatcgctt gtcttctgta
       61 tttaggtgca aagacaatat gttacttaca attccatttc gtatgtgcta aactgtttgt
      121 gtcagatgac attaaatagc atctcctcgt gttgattatt ttggttggct gaccaatatt
      181 tactctaaca tgtagagatg catttttttg acaatggtag aaccttttct ggggagtggg
      241 acagaaatga tattttcgca aaatttattt cgccgtccca ccccaacttg cattgtctgt
      301 agaaattggg aatccaattt ctctttgttg gggccccgcc ccaactcgca ttgcctgtag
      361 aatttctttt cgaaattctc tgtgttgggg cccctgacta gaattgaaaa aagcttatta
      421 caagcgcatt ttcgttcagt caattactgc caatataact tcgtagatca tagaagattg
      481 atttatttcc cagcctattc tttttataat atttagcaaa ggtaataatg atattaataa
      541 tgtacaaaaa atataaatca aatcgacatc cttataaaac atcagaacca ctaaaaacaa
      601 aaaagcacaa aataaaatta aatttaaaat aaacgaccac ttttcaaaaa aatctctttt
      661 catatttcca cccctaattt taataagcat tattttatat tctcttttaa gtttattatt
      721 caaaaggaaa acagaaatat ctttcaatat tattataaac attt
//
LOCUS       DAKTIO010000364          736 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.380, whole genome shotgun sequence.
ACCESSION   DAKTIO010000364 DAKTIO010000000
VERSION     DAKTIO010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.380"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            220..>736
                     /locus_tag="O0293_002922"
     CDS             220..>736
                     /locus_tag="O0293_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkyA family protein"
                     /protein_id="HCY0281724.1"
                     /translation="MKFGKTIAVVLASSVLLAGCTTDKKEIKAYLKQVDKIKDDEEPI
                     KTVGKKIAELDEKKKKLTEDVNSKDTAVRGKAVKDLIKNADDRLKEFEKEEDAIKKSE
                     QDFKKAKSHVDNIDNDVKRKEVKQLDDVLKEKYKLHSDYAKAYKKAVNSEKTLFKYLN
                     QNDATQQGVNEK"
BASE COUNT          324 a           73 c          132 g          207 t
ORIGIN      
        1 agatatgcta attgcttaaa aaatgattaa agtgttggtt tcaagcaatg atactttaga
       61 aatttattta tcatcttgac tttaaaaatt atattataaa tgacgtaact gtcaacagat
      121 atacttagta atgaagatgt gtaatgtaat tgtttaaaat tgatttccaa gcagatttta
      181 tttatcattt aatttaaata gcaagtggag gtacaagtaa tgaaatttgg aaaaacaatc
      241 gcagtagtat tagcatctag tgtcttgctt gcaggatgta ctacggataa aaaagaaatt
      301 aaggcatatt taaagcaagt ggataaaatt aaagatgatg aagaaccaat taaaactgtt
      361 ggtaagaaaa ttgctgaatt agatgagaaa aagaaaaaat taactgaaga tgtcaatagt
      421 aaagatacag cagttcgcgg taaagcagta aaggatttaa ttaaaaatgc cgatgatcgt
      481 ctaaaggaat ttgaaaaaga agaagacgca attaagaagt ctgaacaaga ctttaagaaa
      541 gcaaaaagtc acgttgataa cattgataat gatgttaaac gtaaagaagt aaaacaatta
      601 gatgatgtat taaaagaaaa atataagtta cacagtgatt acgcgaaagc atataaaaag
      661 gctgtaaact cagagaaaac attatttaaa tatttaaatc aaaatgacgc gacacaacaa
      721 ggtgttaacg aaaaat
//
LOCUS       DAKTIO010000365          733 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.381, whole genome shotgun sequence.
ACCESSION   DAKTIO010000365 DAKTIO010000000
VERSION     DAKTIO010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.381"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            151..>733
                     /locus_tag="O0293_002923"
     CDS             151..>733
                     /locus_tag="O0293_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HCY0281725.1"
                     /translation="MFSKKKDKFMVQLEEMVFNLDRAAIEFGKMDFNTHLDLKAYSDN
                     IKTYESHGDELVHQVITDLNQTFITPIEREDILSLCDAIDDVLDAIEETAAMFEMYSI
                     EYTDEYMAEFVDNIQKAVAEMKLAVGLLVDKKLSHMRIHSINIKEFETNCDGILRQSI
                     KHIFNSETDPITLIKIKDIYESMEEIADKCQIVA"
BASE COUNT          285 a           87 c          119 g          242 t
ORIGIN      
        1 atttttgaaa ttaatatcag tacactaaaa ttatactgac gattgataat aacaaatttg
       61 tatcattagt ttgtaaattc acttgtctta atttgaaaca aattataact gaatgtgatt
      121 ggtgacaatc gcttaaatgg aggattttaa atgtttagta agaaaaaaga taagtttatg
      181 gttcaattag aagagatggt tttcaatctg gatcgtgctg ctattgaatt cggtaaaatg
      241 gatttcaata cacatttaga tttaaaagca tactcagaca acattaaaac ttatgagtca
      301 catggtgacg aattagtaca tcaagtaatt actgatttaa atcaaacatt tatcacacca
      361 attgaacgtg aagatatttt atcattatgt gatgcaattg atgatgtttt agatgcaatt
      421 gaagaaacgg cagctatgtt tgaaatgtat tcaatcgaat acacagatga atatatggct
      481 gagtttgttg ataacattca aaaagcagtt gcagaaatga aacttgctgt cggcttatta
      541 gtcgataaaa aattatcaca tatgcgtatt cattcaatta atattaaaga atttgaaaca
      601 aactgtgatg gtattttaag acagtcaatt aaacatattt tcaatagcga aacagatcca
      661 atcactttaa ttaaaataaa agatatttat gaaagcatgg aagaaatcgc tgataaatgt
      721 caaatcgtag caa
//
LOCUS       DAKTIO010000366          726 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.383, whole genome shotgun sequence.
ACCESSION   DAKTIO010000366 DAKTIO010000000
VERSION     DAKTIO010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.383"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            77..697
                     /locus_tag="O0293_002924"
     CDS             77..697
                     /locus_tag="O0293_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281726.1"
                     /translation="MKFTEVEVIEHLVKAYKEAGKPTYPHENLYRGRNHSISGIGEDL
                     LGAYLISRLEGVQIFIDQPLSMIDKSLSTRYPDLLICEDNEIKNILEVKMDLGYQRKD
                     FIDYCRKKEEWISNIVGKQCVLSRKREDKIPMNIADDIKFHVVIYSENNGPKRFDEEI
                     MPIVNETCPHIEVYVLTSGQHPNLVNVNLEGININKDEFEILVNAL"
BASE COUNT          271 a           78 c          142 g          235 t
ORIGIN      
        1 ttgtatttat gctttcaaat gtgtgatata ctttgtttgt tgaaatatag agtatctata
       61 gatagggtga ttgagtatga aattcactga agtggaagtt atcgaacatc ttgtaaaggc
      121 atataaagaa gcaggaaagc ctacttatcc tcatgaaaat ttatatcgag gacgtaatca
      181 tagtatttca ggtattggag aagacttgct gggtgcttat ttgattagta gattggaagg
      241 tgtccaaata tttattgatc agcctttatc tatgattgat aaatctttaa gtacaagata
      301 tccggattta ttaatttgtg aagataatga aattaaaaat atactagaag ttaaaatgga
      361 cttaggatat caaagaaaag attttataga ttattgccga aagaaagaag aatggatttc
      421 aaatatcgta ggaaaacagt gtgtattgtc tagaaagaga gaagacaaaa ttcctatgaa
      481 tatagctgat gatattaaat ttcatgttgt gatttacagt gaaaacaatg gaccgaagcg
      541 gtttgatgaa gaaatcatgc ctatcgttaa tgaaacatgt ccacatattg aagtatatgt
      601 cctaacaagc ggtcaacacc ctaatttagt aaatgttaat cttgaaggta ttaatattaa
      661 taaagatgaa tttgaaatat tagtaaatgc gttataaaaa aatagagcat cctccacgtt
      721 atggag
//
LOCUS       DAKTIO010000367          724 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.384, whole genome shotgun sequence.
ACCESSION   DAKTIO010000367 DAKTIO010000000
VERSION     DAKTIO010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.384"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            65..190
                     /locus_tag="O0293_002925"
     CDS             65..190
                     /locus_tag="O0293_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281727.1"
                     /translation="MRYIHFSIITLITGIIMHITMYFVPFESRKMPLFMTIIFTI"
     gene            452..724
                     /locus_tag="O0293_002926"
                     /pseudo
     CDS             452..724
                     /locus_tag="O0293_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500926.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          286 a           73 c          118 g          247 t
ORIGIN      
        1 tataattaga tgattaatat gtcttggaat taattattta ataagttgta tagcaggtgg
       61 cgatatgaga tatatacact tttctattat tacattaatt acaggtatca ttatgcatat
      121 tacaatgtac tttgtaccat ttgaatctcg gaaaatgcca ctatttatga cgataatttt
      181 tacaatctaa aagcagtaaa gactcataat ataaccaaaa acattaataa aagtttgtta
      241 aatgataagg ggatgagtat tggtgaattt actcaaaagg tttaagggaa aaatcctacc
      301 tggagagatt caaaaactaa atggactatt tccaaaaata aagggcaatc acatggtggt
      361 tcttattgga agttaataaa taacaaagga aaaagaatag cttctttaac taaagaagga
      421 aaaatcttaa gggaataagg tgttgtaaat tatgtgcttt gatataaata atttggatat
      481 aaaaaagttg aattttagaa aggtaaaaaa cgcaattcat ttaagtttaa gtggcgataa
      541 ataccagttt ttggaggacg gtaaaactat caataatact tatttttagc tgtatatgat
      601 aatgcaataa acatttttga agatttgttt tcgccttctg attctataaa tttggttcat
      661 gtagtatatg tttatagtga accttataag aaaacaaatg ttataaaaaa atttacatcg
      721 ttaa
//
LOCUS       DAKTIO010000368          697 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.385, whole genome shotgun sequence.
ACCESSION   DAKTIO010000368 DAKTIO010000000
VERSION     DAKTIO010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.385"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(245..436)
                     /locus_tag="O0293_002927"
     CDS             complement(245..436)
                     /locus_tag="O0293_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LSM domain protein"
                     /protein_id="HCY0281728.1"
                     /translation="MNLQSYIGKLVKLTLTNNKILIGKVIDFDDKVDNFDGYNSIEID
                     TGRITYDISENKIKTIVLQ"
     gene            complement(443..>697)
                     /locus_tag="O0293_002928"
     CDS             complement(443..>697)
                     /locus_tag="O0293_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281729.1"
                     /translation="KNNSSLLPSYTIIDSNESEKMTKKEFIDIPVLFDDEGNFRIKQV
                     IDYKKIIGKSYVNGKYIETKLGKVHYSKTGFHVVPYIKKE"
BASE COUNT          229 a           92 c           84 g          292 t
ORIGIN      
        1 gatgtttagt ttaaatggaa tgcactattt aagtttttaa tatggagatg tttgtgactt
       61 ttcgatgatt aagattttta taggtgtgca tcatttcaaa taaactttgt gtttaaaatt
      121 gagcttagga aatcgatagg tttagatgag gatattgttg aagttatgtg tcttgtatcc
      181 ttagttgtta taaaagcgca aaaaatagca ccgctttctc tttatctgtg tagaaaggat
      241 gctattattg taaaacaata gttttaattt tattttctga tatatcatat gtaattctac
      301 ctgtatcaat ttctatcgaa ttataaccat caaaattatc aactttatca tcaaagtcta
      361 tcacttttcc aattaatatt ttattattag taagcgttaa tttgactaat ttgcctatgt
      421 atgactgtaa attcatattt aatcactcct ttttaatata cggaactaca tgaaaaccag
      481 ttttagaata atgaacctta cctaatttcg tttcaatata tttaccattc acataagatt
      541 tacctattat ctttttataa tctataactt gttttattct aaaattccct tcatcatcaa
      601 acaaaactgg aatatcaata aattcttttt tagtcatctt ttcactttca ttagaatcta
      661 ttatagtata acttggtaat aaagatgaat tattttt
//
LOCUS       DAKTIO010000369          696 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.386, whole genome shotgun sequence.
ACCESSION   DAKTIO010000369 DAKTIO010000000
VERSION     DAKTIO010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.386"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..371)
                     /locus_tag="O0293_002929"
     CDS             complement(<1..371)
                     /locus_tag="O0293_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4865 family protein"
                     /protein_id="HCY0281730.1"
                     /translation="MHAMQYHVKLPSDYNMEIIRDRVRLNGYKTDGFKNLIIKAYLIS
                     QTTTNCITNTYSPLYLWRSSEGMTEFIFNGFYDNVISSFGWQNINIGVIYSMNITDSV
                     KHSLYALEEYIDIFPTLSLKE"
     gene            462..>696
                     /locus_tag="O0293_002930"
     CDS             462..>696
                     /locus_tag="O0293_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0281731.1"
                     /translation="MIKLNFNDLEIFITVCEEASINKAAIKLRYAQSNISQRISKLEN
                     ELGVVLLFRNQKGAKATKAGEEFLAYSKKVLRDT"
BASE COUNT          268 a           95 c           97 g          236 t
ORIGIN      
        1 atttctttta gagataaagt agggaaaatg tcgatatatt cttcaagcgc ataaagacta
       61 tgtttaacag aatcggttat gttcattgaa tatatcacac caatatttat attttgccat
      121 ccaaaagaag aaataacatt atcataaaat ccattgaata taaattcagt catcccctct
      181 gaacttctcc ataaatataa tggtgaataa gtatttgtaa tgcaattagt agttgtttga
      241 cttattaaat aagcttttat aattaaattt ttaaatccat cagtcttata accatttaat
      301 cgaactctat ctctaattat ttccatattg taatcactag gcaacttaac atgatattgc
      361 attgcatgca tttagcatac ccccttttat aaaaaggata gcaataataa gtaaaatctc
      421 atattatcca attgtgatat agttatcata aaaagtgata ggtgattaaa ttgaacttta
      481 atgatttgga aatttttata actgtatgtg aagaagcatc tatcaataaa gctgcaatta
      541 aacttagata tgcacaatct aatatatctc aaagaattag caagcttgaa aatgaattag
      601 gtgtagtttt gctttttaga aatcaaaaag gtgctaaggc aactaaagca ggcgaagaat
      661 tcttagcgta tagcaaaaaa gtattaagag atacag
//
LOCUS       DAKTIO010000370          696 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.387, whole genome shotgun sequence.
ACCESSION   DAKTIO010000370 DAKTIO010000000
VERSION     DAKTIO010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.387"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..487)
                     /locus_tag="O0293_002931"
     CDS             complement(<1..487)
                     /locus_tag="O0293_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0281732.1"
                     /translation="MNKVTINPQIQLTYQIEGKGDPIILLHGLDGNLAGFEDLQHQLA
                     SSYKVLTYDLRGHGKSSKSESYDLNDHVEDLKILMEKLNIHEAHILGHDLGGVVAKLF
                     TDKYAYRVKSLTTIASKKDDLIHSFTQLLIQYQDDIAGFNKSEAYILLFSKLFRNQEK
                     TM"
BASE COUNT          242 a          120 c           77 g          257 t
ORIGIN      
        1 tcatcgtctt ctcttgattt ctaaacaatt tagaaaataa aagaatatac gcttcagact
       61 tattaaaacc cgctatatca tcttgatatt gtattaacaa ttgagtaaag ctgtgtatta
      121 agtcatcttt cttcgatgca atggtagtta atgattttac acgataagca tatttatctg
      181 taaataactt agcaactacc ccacctaaat catgtcctag aatatgtgcc tcatgaatat
      241 ttaacttctc cattagaatt tttaaatcct caacgtgatc gtttaaatcg tatgattcac
      301 ttttagaaga cttgccatga cctcttaaat cgtaagtaag tactttatat gatgatgcta
      361 gttgatgttg caaatcttca aatccagcta aattaccatc caatccatga agtaatatta
      421 taggatcccc tttaccttca atttgataag ttaattggat ttgaggatta attgtgactt
      481 tattcattgt gacacctccc tcaataaact atatacccaa ccttcattct atatacgctt
      541 ccattttata aacaattttt ttaaattatt tataaattac caaaagttat tccaacaata
      601 aaaaaactat tcatctcaat taatacaatt aatcaagtga atagttttat catatttata
      661 atcttgtacg tgaagataat ctaattgtga aatata
//
LOCUS       DAKTIO010000371          694 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.389, whole genome shotgun sequence.
ACCESSION   DAKTIO010000371 DAKTIO010000000
VERSION     DAKTIO010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.389"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(237..632)
                     /gene="spxA"
                     /locus_tag="O0293_002932"
     CDS             complement(237..632)
                     /gene="spxA"
                     /locus_tag="O0293_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCY0281733.1"
                     /translation="MVTLFTSPSCTSCRKAKAWLQEHDIPYTERNIFSEHLTIDEIKQ
                     ILKMTEDGTDEIISTRSKTYQKLNVDIDSLPLQDLYSIIQDNPGLLRRPIILDNKRLQ
                     VGYNEDEIRRFLPRKVRTFQLQEAQRMVD"
BASE COUNT          242 a          103 c           99 g          250 t
ORIGIN      
        1 ctatttaaat gatttataca gttaaagcat taaagcactt tcttaattta attttacctt
       61 atcatagcaa atgtttcatc caaaacactc taatatagag ctatattaac aattgcacat
      121 ttgatttaaa agatactaag caattattgt aaaattaaaa ccaaaaaagc aacactatag
      181 aaatagtgtt gcttgacata tcttttagta aagtatgaca catagttaaa tggttattag
      241 tcaaccatac gttgtgcttc ttgtaattgg aacgtacgaa cttttctagg taagaaacgt
      301 cgaatctcgt cctcattata accaacttgt agtcgtttat tatctaaaat aattggacga
      361 cgtaataagc caggattatc ttgaatgatt gaatataagt cttgtaatgg tagtgaatca
      421 atatcaacat ttaatttttg gtatgtttta gaacgtgtag aaatgatttc atcagtaccg
      481 tcttcagtca tttttaatat ttgcttaatt tcatcaattg ttaaatgttc agaaaaaata
      541 ttacgctccg tatacggaat gtcatgttct tgtaaccatg ctttcgcttt acggcaagat
      601 gtgcaacttg gtgaagtaaa taatgttacc atacatctca ctctcctatt tgaatgaata
      661 aaattcattg cttaaaattt agttatagat caag
//
LOCUS       DAKTIO010000372          691 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.390, whole genome shotgun sequence.
ACCESSION   DAKTIO010000372 DAKTIO010000000
VERSION     DAKTIO010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.390"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(73..651)
                     /gene="pgsA"
                     /locus_tag="O0293_002933"
     CDS             complement(73..651)
                     /gene="pgsA"
                     /locus_tag="O0293_002933"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCY0281734.1"
                     /translation="MNIPNQITVFRVVLIPVFILFALVDFGFGNVSFLGGYEIRIELL
                     ISGFIFILASLSDFVDGYLARKWNLVTNMGKFLDPLADKLLVASALIVLVQLGLTNSV
                     VAIIIIAREFAVTGLRLLQIEQGFVSAAGQLGKIKTAVTMVAITWLLLGDPLATLIGL
                     SLGQILLYIGVIFTILSGIEYFYKGRDVFKQK"
BASE COUNT          271 a          130 c           73 g          217 t
ORIGIN      
        1 tggctaattt ctaaaattgg gattaaaaaa actagattcc atatgaaaat gaaatctagt
       61 ataaacaaat atttattttt gtttaaaaac atctctacct ttataaaagt attcaatacc
      121 agataagata gtaaaaataa cgccaatgta taataaaatt tgtcctaatg acaaaccaat
      181 caatgttgcc aatggatcac ctaataacaa ccaagtaatt gctaccatag taactgctgt
      241 tttaatttta cctaattgac cagctgcact tacgaatcct tgttcaattt gtagtaaacg
      301 taaaccagtt acggcaaatt ctctggcaat aataatgatt gctactacag aatttgttag
      361 tcctagttgc acaagtacaa ttaaagcact tgcaactaat aatttatccg ctaatggatc
      421 caaaaatttc cccatatttg taactaaatt ccattttcta gctaaataac catcaacaaa
      481 atcgctaagg gaagccaata taaaaataaa accactgatt aataactcaa ttcttatttc
      541 atatcctcct agaaatgaca cattgccaaa tccaaaatca actaacgcaa acaatataaa
      601 aactggtatt aacactactc taaaaaccgt aatctggttc ggaatattca ttatacatcc
      661 tcatttctca ctaatttatt tctgttaaaa t
//
LOCUS       DAKTIO010000373          663 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.391, whole genome shotgun sequence.
ACCESSION   DAKTIO010000373 DAKTIO010000000
VERSION     DAKTIO010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.391"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            70..612
                     /locus_tag="O0293_002934"
     CDS             70..612
                     /locus_tag="O0293_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCY0281735.1"
                     /translation="MVRESIPKEGENIKIQSYKHDGKIHRVWSETTILKGTDHVVIGG
                     NDHTLVTESDGRTWITREPAIVYFHSEYWFNVICMFREDGIYYYCNLSSPFVCDEEAL
                     KYIDYDLDIKVYPNGKYHLLDEDEYEQHMNQMNYPHDIDIILRRNVDILQQWIEQKKG
                     PFAPDFIKVWKERYKKIRQY"
BASE COUNT          247 a           78 c          129 g          209 t
ORIGIN      
        1 ttatataata ctatttctga aatatgctat aatttataat ggttaaatta acagaaggtg
       61 gtgtggagta tggtcagaga atccatacct aaagaagggg aaaacattaa aattcagagt
      121 tataaacatg atggtaagat tcatcgcgtt tggtctgaaa caacaatatt aaagggtact
      181 gatcatgttg tcattggtgg aaatgatcat acgttagtga ctgaaagtga cggaagaact
      241 tggataacac gtgaaccggc aattgtttac tttcactcag aatattggtt taatgttatc
      301 tgcatgttta gagaagatgg aatttattac tattgcaatt tgtcatcacc atttgtttgt
      361 gatgaagagg cattaaaata tatagactat gatttagata ttaaagtata tccaaacgga
      421 aagtatcatt tattagatga agatgaatat gagcaacata tgaatcaaat gaattatccg
      481 catgatattg atattatttt aagacgcaac gtggacattc ttcaacaatg gatagaacag
      541 aagaagggcc cattcgcgcc agattttatc aaagtttgga aagaacgtta caaaaaaatt
      601 agacagtatt aagcaaataa agtgtaaata ctgattaaat catttatatt gacttagtga
      661 tgt
//
LOCUS       DAKTIO010000374          652 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.392, whole genome shotgun sequence.
ACCESSION   DAKTIO010000374 DAKTIO010000000
VERSION     DAKTIO010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.392"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..>652
                     /locus_tag="O0293_002935"
     CDS             <1..>652
                     /locus_tag="O0293_002935"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCY0281736.1"
                     /translation="GKKAGEFYTPQQVSKILAKIVTDGKDKLRHVYDPTCGSGSLLLR
                     VGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYENFDIRNDDTLENPAFLGNTFDAV
                     IANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAFIQHMVHYLDDEGTMAVVLPH
                     GVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCILVFKKCRQQDDNVL"
BASE COUNT          209 a          121 c          138 g          184 t
ORIGIN      
        1 ggtaaaaaag caggcgagtt ctatacacca caacaagtat ctaagatact ggcgaagatt
       61 gtcacagacg gtaaagataa attacgtcac gtgtatgacc caacatgtgg ttcaggttca
      121 ctgttgttac gtgttggtaa agaaacacaa gtgtatcgtt atttcggtca agaacgtaac
      181 aatactacat acaacttagc acgcatgaat atgttattac atgatgtgcg ttatgagaac
      241 ttcgatatcc gtaatgatga cacattggaa aacccagcct ttttaggcaa tacatttgat
      301 gcggttattg cgaacccacc gtatagtgcg aaatggactg cagattcaaa gtttgaaaat
      361 gacgaacgat tcagtggtta cggcaaactt gcgcctaagt ctaaagcaga ctttgccttt
      421 attcaacaca tggtacatta cctagacgat gaaggtacca tggccgttgt actcccacat
      481 ggtgtattat tccgaggtgc tgcagaaggt gtcattcgtc gttatttaat tgaagaaaag
      541 aactacttag aagctgtgat tggtttgcca gcgaatattt tctatgggac aagtattcca
      601 acatgtattt tagtatttaa aaaatgtcgc caacaagacg acaacgtact at
//
LOCUS       DAKTIO010000375          643 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.393, whole genome shotgun sequence.
ACCESSION   DAKTIO010000375 DAKTIO010000000
VERSION     DAKTIO010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.393"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..>643)
                     /locus_tag="O0293_002936"
     CDS             complement(<1..>643)
                     /locus_tag="O0293_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0281737.1"
                     /translation="STFIAPIVFILLMVAQGYIATKYNEIFTPQESFTSAYNGFSWFA
                     FLLIIQASTIISMEFHYGTIKNLLYREYSRTTMIVSKIITLFIISLIYFVITIIASIV
                     IGSLFFNDLNIFESSGNQLSLLNQLLLVSLGTFVGVWLVLSLTLLLSSATNSTGVAIA
                     VGIVFYFASSILAVIQTALLEKIDWLKWNPINMMNIMLQTVEKGFSKSTKLEL"
BASE COUNT          260 a           93 c           87 g          203 t
ORIGIN      
        1 tgaagttcta attttgtcga cttactaaag cctttttcaa ctgtttgaag cataatattc
       61 atcatattaa taggattcca ctttagccag tctatttttt ctaaaagtgc cgtttgaata
      121 actgctaaaa tagaacttgc aaaataaaaa acaataccta cagcaatggc tactcccgtt
      181 gaatttgttg cagatgataa tagcaacgtt aagcttaaaa ctaaccaaac gccaacaaat
      241 gtgcctaaac taactaataa taattgattc aataaagata attgattacc gctactttca
      301 aatatattta aatcattaaa gaataaagac ccaataacaa ttgaagcaat aattgtaata
      361 acaaaataaa ttaaagaaat aataaataat gtgatgattt tgctaacaat catagttgtt
      421 cttgaatatt cacgatagag taaattttta atcgtaccgt aatgaaattc cattgaaatg
      481 attgtacttg cttgaataat taataaaaat gcaaaccatg aaaaaccatt ataagcagat
      541 gtgaaagatt cctgtggcgt aaaaatttca ttgtattttg tagcaatata accttgagca
      601 accattagta gaataaagac aataggtgcg ataaaagttg att
//
LOCUS       DAKTIO010000376          609 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.394, whole genome shotgun sequence.
ACCESSION   DAKTIO010000376 DAKTIO010000000
VERSION     DAKTIO010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.394"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            161..>609
                     /locus_tag="O0293_002937"
     CDS             161..>609
                     /locus_tag="O0293_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenyl diphosphate synthase component 1"
                     /protein_id="HCY0281738.1"
                     /translation="METTVSKLERQIEERLKGVSEYESININHRLGKLLDLYDIPDVA
                     KVACLTIDTSMRHLDDITYNHLSKHSILIGDLISAHFYTLLAEINDLSFQNEISKAIV
                     EINELKSSLHHQALNDYEISQAIVKIETLFPYITLSHFGINIDESEI"
BASE COUNT          238 a           74 c           91 g          206 t
ORIGIN      
        1 aaatgaagta aaggttatta tgagaattac aaaagctaca taaattactg ttagtttaaa
       61 ttgaaattta aaaatgataa gtaatcttaa tgcttcaaaa gattttaatt tcttacatat
      121 aatgttaata tgaatagtga taatatcgtg aggtgtagac atggaaacaa ctgttagcaa
      181 attggaaaga caaatagaag aaagattaaa gggcgtatca gaatatgaat ccattaatat
      241 taatcatcgg ttaggtaagt tacttgattt atatgatatt ccagatgttg ccaaagtggc
      301 atgcctcaca attgacacat caatgagaca ccttgatgac attacatata atcatttgtc
      361 taaacattcc attttgattg gagatttaat tagtgcacat ttttatacgc tactagcaga
      421 aatcaatgat ttatcatttc aaaatgaaat tagtaaagca attgttgaaa ttaatgaatt
      481 gaaatcttca ttacatcatc aagctttaaa tgattatgaa atttctcaag caatagtaaa
      541 aattgaaaca cttttcccat atattacgtt atctcatttc ggtatcaata ttgatgagtc
      601 ggaaatata
//
LOCUS       DAKTIO010000377          598 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.397, whole genome shotgun sequence.
ACCESSION   DAKTIO010000377 DAKTIO010000000
VERSION     DAKTIO010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.397"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(13..513)
                     /locus_tag="O0293_002938"
                     /pseudo
     CDS             complement(13..513)
                     /locus_tag="O0293_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532440.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          190 a          101 c           98 g          209 t
ORIGIN      
        1 ttttataaat ctttagattg tcaaattaag ctggacaagt ttatcatctt cgatgaaact
       61 cataaatact tccaatggtg ttctataacc taaagatttt ctaggatgat gattgatttt
      121 ttgagcaaca ctgatgatat gatcatccgt tactaaattg aaatctagtt ctttatctaa
      181 tccactgcgt ctaagtattc cgttagaatg ttcatttaaa cctcgctgtg atggcgtgcc
      241 tggatcagca aaaaatatag atatatcgtg acagttacat aatgttttcc agtttgagaa
      301 ctccttacca caatcaaaag taattgattt aaatatatga gaaggtaaag atgaaaacat
      361 tgagtgtaga gcgttttcta tatcagttgc cttacgacct aagggtcgta aggtgataat
      421 catcttggat actctttcta ccaaagtaat gattgcacta ccatgacgac gaccaacaat
      481 agtatctcct tcaagatgtc caaactcctt atgttttctt tggtaggaaa tacatattga
      541 gtttagcaca taaggcttat ttatttacct ttttcttcac gacgttttaa ataatagg
//
LOCUS       DAKTIO010000378          584 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.399, whole genome shotgun sequence.
ACCESSION   DAKTIO010000378 DAKTIO010000000
VERSION     DAKTIO010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.399"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            13..147
                     /locus_tag="O0293_002939"
     CDS             13..147
                     /locus_tag="O0293_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001806232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281739.1"
                     /translation="MKDSNIISFIYSRHDRILYVNLVGNRFKNNISMTKATSNIGRGF
                     "
     gene            complement(286..477)
                     /locus_tag="O0293_002940"
     CDS             complement(286..477)
                     /locus_tag="O0293_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281740.1"
                     /translation="MRKEIEALIFSDVSSYDIYVNTGVNQGLIGDIKDGYLTIDSMPY
                     IDAERLYHFAMERKSLVTN"
BASE COUNT          197 a          104 c           92 g          191 t
ORIGIN      
        1 aacaagttga aaatgaagga ttctaatatc atttcgttta tatatagcag acatgataga
       61 attttatatg taaatcttgt aggtaatcgt tttaaaaata atataagtat gactaaagcc
      121 acatccaata taggacgtgg cttttagtat cgttccagat ttgtggggag ttagggttta
      181 cttaattaaa ggggtctgcc ctcaattgct caccaacgag ggcaagttac atcaatcaca
      241 cttcaattgc cgccgactag gggtagtaat cattggcaat aagaactagt tagtgactaa
      301 cgatttacgt tccatagcaa agtgatacaa acgctcagca tcaatgtaag gcatagaatc
      361 aatagttagg taaccatctt tgatgtcacc aattaatcct tgatttacac cagtgttcac
      421 gtaaatatca tagctactta cgtctgagaa aataagcgct tcaatctctt ttctcataat
      481 catcactcca atgtttatat agtatttata taaactctct cgtctctctc tatttattaa
      541 cttcttacaa gtctaatatt acatgaattt ccaaataaat aaag
//
LOCUS       DAKTIO010000379          571 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.401, whole genome shotgun sequence.
ACCESSION   DAKTIO010000379 DAKTIO010000000
VERSION     DAKTIO010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.401"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..>571
                     /locus_tag="O0293_002941"
     CDS             <1..>571
                     /locus_tag="O0293_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 1"
                     /protein_id="HCY0281741.1"
                     /translation="QVFYDEKGYPYISRNINANNGAVGKTYVLVNKKEFKNNLALCVY
                     YLEKLIKDSKDSIMICDGPGSFPKMFNTNHKNAQKYGVIHVNHHENFDDTGAFKKSEK
                     YIIENANKINGVIVLTEAQRLDILNQFDVENIFTISNFVKIHNAPKHFQTEKIVGHIS
                     RMVPTKRIDLLIEVAELVVKKDNAVKFHI"
BASE COUNT          228 a           58 c           96 g          189 t
ORIGIN      
        1 atcaagtatt ttatgatgaa aagggatacc catatatttc aaggaatatt aatgctaata
       61 atggtgctgt aggtaaaact tatgtgttag ttaataaaaa agaatttaaa aacaatttag
      121 cactgtgtgt ttactattta gaaaaactaa taaaagattc taaagatagt attatgattt
      181 gtgatggacc agggagtttt ccaaaaatgt ttaatacaaa tcataaaaat gctcagaaat
      241 atggcgttat tcatgttaat catcatgaaa atttcgatga tacgggtgca tttaaaaaaa
      301 gtgagaaata tattattgag aatgcgaata aaattaacgg tgtaattgta ttaacagagg
      361 cacaaagatt agatattctt aatcaatttg atgtagaaaa tattttcact attagcaatt
      421 ttgttaagat acataatgct ccaaaacatt ttcaaactga aaaaatcgta ggtcatattt
      481 ctagaatggt accaacgaag cgaattgatt tgcttattga agtggctgag ttagtcgtaa
      541 aaaaagataa tgctgttaaa tttcatatat a
//
LOCUS       DAKTIO010000380          569 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.402, whole genome shotgun sequence.
ACCESSION   DAKTIO010000380 DAKTIO010000000
VERSION     DAKTIO010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.402"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
BASE COUNT          249 a           66 c           70 g          184 t
ORIGIN      
        1 aaataacaat aacaaatttc aaccgataag ataaaatgtc ttgtcggttt ttaatataac
       61 aagtgatatt ttggaaatgc caaaaatata tagattgttt atataaaatg atttaaaatg
      121 ttttataaca acatatttta tgcaacacat aaaatgggta actaacatca cgaaatcatt
      181 gtatttgttg ttacactgat gaagcgatgt cacaaagaaa tgtcgttatc aaacgaagcc
      241 cgaaatacat actgatactc gaaataatgt aacgataaat aacaatgata acgattgatt
      301 actacgcaaa aatatatgta aattgacgat ttaaatgcaa ttcgtatatt ttgccaatga
      361 tttacacatt aaaatgtttg aaaattgtca caattcagtc taatttaaaa ggaagttaat
      421 tacaaaatta aaaaatcatg catcgtgtca tttagatatt gaaaaagatg agtcaaattt
      481 gtttcatata ttttccaata acaaattatt atatctaaat atacaatcag aaatatatga
      541 atagaaatat caataagaaa aataatatg
//
LOCUS       DAKTIO010000381          567 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.403, whole genome shotgun sequence.
ACCESSION   DAKTIO010000381 DAKTIO010000000
VERSION     DAKTIO010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.403"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
BASE COUNT          208 a          105 c           62 g          192 t
ORIGIN      
        1 aactatccat ctttaataag aaatgaacta gtgttttaat ctattacatt aaaaaaacta
       61 cctaaacagg tagtttttta tttattttag gccgattaaa tgtcttatgt ctttaattaa
      121 gtaaaatgca caagtacacc tttaaaaaga tttactcaaa actaaactat acctagttaa
      181 ccgcttgtga ttaagaaatt ttaaatgtgg tttcttttaa ttatgtcaac cacccataag
      241 gattttaatc aagcactcat tcattcctgc actcgcaata tgcattactt atagaagtgg
      301 atgcactctc atattatggc aataccataa ctcccgcacc ataatgattt aaataccatt
      361 atgaatctca attacaaatt agcacataaa aaagacgcct cccataagtc tagtcaccaa
      421 atggaaaacg tctcgtaatt aaaatatatt tgcgtgcatt ctaatttgta cttagaatac
      481 atcatactcg ttcataatgt aattttctaa ttagtcaaaa ctatatacag ttttacatca
      541 ttcccggcat tatgcactaa taaaaac
//
LOCUS       DAKTIO010000382          557 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.404, whole genome shotgun sequence.
ACCESSION   DAKTIO010000382 DAKTIO010000000
VERSION     DAKTIO010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.404"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            175..531
                     /gene="sarT"
                     /locus_tag="O0293_002942"
     CDS             175..531
                     /gene="sarT"
                     /locus_tag="O0293_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarT"
                     /protein_id="HCY0281742.1"
                     /translation="MNDLKSKSNIKLMKRVLTTYELRKYLKKYFCLTLDNYLVLAYLD
                     VFKNDEGKYFMRDIISYIGIDQSRIVKSVKDLSKKGYLNKCRDPHDSRNVIIVVSVKQ
                     HNYIKNLLSEININET"
BASE COUNT          237 a           49 c           82 g          189 t
ORIGIN      
        1 atgacaaatt catttattat ccaaaaataa aaacaattga acaaaatcaa aatatagtgt
       61 tatgattcat ttgtgtttat agctagcacc acaaactaaa tatatttgga gttattacta
      121 gttaaagaca aatattattt gacgtgacaa ttaaaagtaa gggatgaaga cttgatgaat
      181 gatttgaaaa gcaagagcaa tattaaatta atgaaaagag tattaactac atatgagctg
      241 cgtaaatatt taaaaaaata tttttgtctt acattagata attatttagt attagcatat
      301 ttagatgtat ttaaaaatga tgaaggtaaa tattttatga gagacattat aagttatatc
      361 ggtatagacc aatctagaat tgttaaaagc gtaaaagatt tatcaaaaaa gggttacttg
      421 aataaatgta gagacccaca tgatagtaga aatgtaatca ttgttgtttc tgtgaaacaa
      481 cacaattata ttaaaaatct tttgagtgaa ataaatatta atgaaacata aagtatgttt
      541 cgagatttta atatctt
//
LOCUS       DAKTIO010000383          554 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.405, whole genome shotgun sequence.
ACCESSION   DAKTIO010000383 DAKTIO010000000
VERSION     DAKTIO010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.405"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            1..554
                     /locus_tag="O0293_002943"
                     /pseudo
     CDS             1..554
                     /locus_tag="O0293_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
BASE COUNT          220 a           65 c           85 g          184 t
ORIGIN      
        1 atgttgaaaa tcataaactg cttaataata aagaacgaga tctaagtaat agtgctccat
       61 taatttataa gacattaagt gcgctgtatc acaactttga tggctttgga ttaaacattt
      121 ttgacaatca tcaagcattt aatgcgatgc atctatatga caaaaatgga tttaaaacaa
      181 cactaggtct tatattagaa aagtttatcg aatatgtctc gaaaccaaaa tacgaaaaca
      241 gttattattc tatttttgat atagagaatt attattgtct tgttatttat gattggcgag
      301 tgatagagag cgagacaatt atgagtaatt ttgaggatag tcaagtttat ataaatttta
      361 aaaacaatgt tttaaacgat aaatatctaa ttgtaataga aacattggac aaaatagtgg
      421 caacattaat catttgattt ctaaagaatt aagagataaa tatgaatgga accctagttt
      481 actatctaaa attgacaact accttaaacc agcaatagag attaaagagc ataattttag
      541 tgataattcc ttga
//
LOCUS       DAKTIO010000384          544 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.406, whole genome shotgun sequence.
ACCESSION   DAKTIO010000384 DAKTIO010000000
VERSION     DAKTIO010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.406"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            123..>544
                     /locus_tag="O0293_002944"
     CDS             123..>544
                     /locus_tag="O0293_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCY0281743.1"
                     /translation="MYKNYNLIQLTLPIETSVRIPQIDISRYVNEIVETIPDSEFDKF
                     RHHRGATSYHPKMMLKITLYAYTQSVFSGRRIEKLLHDSIRMMWLAQNQTPSYKTINR
                     IRVNPNTDALIESLFIQFHSQCLKQNLIDGTKVEANAN"
BASE COUNT          200 a           88 c           74 g          182 t
ORIGIN      
        1 aaaaaataag acactttgcc aaacttgcac ataaatgttt aattcaataa tttgaatttt
       61 ctgtgttggg tcccttcgta taatttaata aataccacta aactaaatta atgaagtgcc
      121 ttatgtataa aaattataac ttgatccaac ttacactacc aatagaaact tctgttagaa
      181 ttcctcaaat tgatatttcg cgatatgtta atgaaattgt tgaaacgata cctgatagcg
      241 aattcgataa attcagacat catcgtggcg caacatccta tcatccaaaa atgatgttaa
      301 aaatcacctt atatgcatat actcaatctg tattttctgg tcgtagaata gagaaattac
      361 ttcatgacag tattcgaatg atgtggttag ctcaaaatca aacaccttct tataaaacta
      421 ttaatcgtat tagagtgaat cctaatactg atgcgttaat tgaatcttta tttatccagt
      481 ttcatagtca atgtttaaag caaaatctta ttgatggtac aaaagtagaa gctaatgcca
      541 atag
//
LOCUS       DAKTIO010000385          544 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.407, whole genome shotgun sequence.
ACCESSION   DAKTIO010000385 DAKTIO010000000
VERSION     DAKTIO010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.407"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..>544
                     /locus_tag="O0293_002945"
     CDS             <1..>544
                     /locus_tag="O0293_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCY0281744.1"
                     /translation="HILMRDYARQTIEYISLSKDISNINLEKIRPPYKSNWYKKEYSN
                     LNIDDYIKSLKNKLDSHLHFSIDKIKNSMTTEYGRGTGAYGDFGRYVFGYAVRNWVKG
                     FKSDQDLSNIALMRIFEMGYDAKLHGEFDMWVNRYDNFNNSIERISKKYQWIAYYEIL
                     AKLVDKFPDVQYSGLWDDYIR"
BASE COUNT          216 a           50 c           96 g          182 t
ORIGIN      
        1 catattttaa tgagagatta tgctaggcaa acaattgaat atattagttt atcaaaagat
       61 atatctaata taaatttaga aaaaattagg ccaccttata aaagtaattg gtataaaaaa
      121 gaatattcga atttaaatat agacgattac ataaaatctt taaaaaacaa attagatagc
      181 catttacatt tttctatcga taaaattaaa aactcaatga caacggaata tggaagagga
      241 actggggcat atggcgattt tggcagatat gtatttggtt atgctgtacg aaactgggta
      301 aagggtttta aaagtgatca agacttaagt aatatagcat taatgaggat ttttgaaatg
      361 ggttatgatg caaaattaca tggtgaattt gatatgtggg ttaatcgtta tgataacttt
      421 aataattcaa ttgaaagaat tagcaaaaaa tatcaatgga ttgcttacta tgaaatttta
      481 gctaaacttg tagataaatt tcctgatgtt caatattctg gactatggga tgattatatt
      541 agag
//
LOCUS       DAKTIO010000386          539 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.409, whole genome shotgun sequence.
ACCESSION   DAKTIO010000386 DAKTIO010000000
VERSION     DAKTIO010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.409"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(201..305)
                     /locus_tag="O0293_002946"
     CDS             complement(201..305)
                     /locus_tag="O0293_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281745.1"
                     /translation="MLNIKAIKPSEKGIKVLSHQFKQVSKTQLNYFFN"
BASE COUNT          181 a           76 c           53 g          229 t
ORIGIN      
        1 tgaaattata ttattacttc tacaaatttc aaattgccgt aattgaacgt atatttcttc
       61 ttcaactatt atttcatctt tagcataatc tatatataaa attttatgct attatttaaa
      121 taattcgcta taacttaaca tacgttttcg atataaacct tgttctaaat ctcaataatt
      181 ttttgctgtt ttcatcgtca ttagttaaaa aaataattta actgagtttt tgagacctgt
      241 ttaaattgat gactcaaaac ctttatcccc ttttcactcg gtttaattgc tttaatattt
      301 aacacagtct cattgtaatt ttgctactaa gtttgaaata tttcgattcg aatgtgaaac
      361 atcatttatt attactttcg acagctagaa aaatattgtt aaaccaaaac tataattacc
      421 acttttatat agaactatat ataaatttta actttgagac agaactgcta gtcaggttta
      481 tgaatatatt tctttagttt acttgatata cttattggta aatcattatt tatttgaag
//
LOCUS       DAKTIO010000387          531 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.410, whole genome shotgun sequence.
ACCESSION   DAKTIO010000387 DAKTIO010000000
VERSION     DAKTIO010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.410"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
BASE COUNT          170 a           84 c           81 g          196 t
ORIGIN      
        1 gaaaagacac tgatgataga caacaaaatt gataacataa ttttccattt actatcagaa
       61 gcaaatccgc tgagtatctt tatccaattt gtctgattgt tcatcgtata tgtaactcct
      121 ttcaattaat aatctaaatt aagccgctta tattatttat ttcactggat gatatacata
      181 atataaattt gttatttgtt aaaaattaat acttattaca agtacatcat atattagttg
      241 ataacgatta tcaatgtcgc gtggatttgt gacacatttc ttttaaaaat tcacaaggtt
      301 atggggcaga aatgataaag agccactaat gatttattat gtagtggttc tgggagtggg
      361 acagaaatga tattttcaca aaatttattt cgtcgtccca ccccaacttg cattgtctct
      421 agaaattggg aatccaattt ctctttgttg ggtccctgaa tatagccttg tagagtctag
      481 tacattgatt tgtatcccaa tgtccctata attgattatt cgctttatct a
//
LOCUS       DAKTIO010000388          531 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.411, whole genome shotgun sequence.
ACCESSION   DAKTIO010000388 DAKTIO010000000
VERSION     DAKTIO010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.411"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(77..385)
                     /locus_tag="O0293_002947"
     CDS             complement(77..385)
                     /locus_tag="O0293_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HCY0281746.1"
                     /translation="MTEHNHDSQLEINNEEELLTLFDEEGNEVLYRKVLEFYHPEFKK
                     EYVILAEEGAQSDEDDMIELVPMINEPDESGDGGKLVPIETDEEWDMIEEVVNTEMEE
                     "
     gene            complement(400..>531)
                     /gene="ruvX"
                     /locus_tag="O0293_002948"
     CDS             complement(400..>531)
                     /gene="ruvX"
                     /locus_tag="O0293_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCY0281747.1"
                     /translation="ERLSTMAAERSLLEADVSRQKRKQVIDKMAAVFILQGYLDSLH"
BASE COUNT          150 a          108 c           61 g          212 t
ORIGIN      
        1 tatttataca ttgcttgact taaaaaatac tcttgcctcc ccatctttaa ggttagcaag
       61 agtaaaatct ttttaattat tcttccattt cagtatttac aacttcttca atcatgtccc
      121 attcttcatc agtttcgatt ggtactaact taccaccgtc acctgactca tctggttcat
      181 tgatcattgg tacaagctca atcatatcgt cttcatctga ttgagcacct tcttcagcta
      241 agataacata ctcttttttg aattcaggat gataaaattc taaaactttt cggtataaaa
      301 cttcatttcc ctcttcatcg aataaagtta ataattcttc ttcgttatta atttctagtt
      361 gtgaatcatg attatgttca gtcatagtaa aatctccttt taatgtagtg aatctaaata
      421 gccttgtaaa ataaataccg ctgccatttt atcaatcact tgttttcttt tttgtcttga
      481 aacatctgct tctaataatg atcgttcagc agccattgtg cttaatcttt c
//
LOCUS       DAKTIO010000389          514 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.414, whole genome shotgun sequence.
ACCESSION   DAKTIO010000389 DAKTIO010000000
VERSION     DAKTIO010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.414"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            213..>514
                     /locus_tag="O0293_002949"
     CDS             213..>514
                     /locus_tag="O0293_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0281748.1"
                     /translation="MKKLLLPLIIMLLVLAACGNQGEKNNKAETKSYKMDDGKTVDIP
                     KDPKRIAVVAPTYAGGLKKLGANIVAVNQQVDQSKVLKDKFKGVTKIGDGDVEKVA"
BASE COUNT          198 a           55 c          100 g          161 t
ORIGIN      
        1 ttttaaaagg taaatcgatc gttgattggc ataaagagat gaaatttgac gttcggattt
       61 accctttttt tgtggggatt tatagggatg ttaagatatt ttgttaaatt ttttgcgaaa
      121 ataaaaaata aataacacta gctattgtaa atgtatatgc aatcttgtat aatttttgaa
      181 tgattgataa tcattttcaa taggaggaaa ttatgaaaaa actattatta ccattaataa
      241 ttatgttatt agtgttagct gcgtgtggga accaaggtga aaaaaataac aaagctgaaa
      301 ctaaatctta taaaatggac gatggcaaaa cggtagatat tccgaaagac cctaaacgca
      361 ttgcagtagt tgcgccaaca tatgctggtg gacttaaaaa attaggtgca aacattgtag
      421 ctgtaaatca acaagtcgat caaagcaaag tattaaaaga taaatttaaa ggtgttacaa
      481 aaattggtga tggcgatgta gaaaaagttg ctaa
//
LOCUS       DAKTIO010000390          506 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.415, whole genome shotgun sequence.
ACCESSION   DAKTIO010000390 DAKTIO010000000
VERSION     DAKTIO010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.415"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            271..506
                     /locus_tag="O0293_002950"
                     /pseudo
     CDS             271..506
                     /locus_tag="O0293_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          204 a           74 c           68 g          160 t
ORIGIN      
        1 tgtaatttta ctttgaaata ctttaaaaaa ataagacact ttgcccaact tgcacataaa
       61 tgtaaaattc aataaaataa attttctgtg ttggatccct tcgtataatt taataaatac
      121 tactaaacta aattaacgag gtgccttatg tataaaaatt ataacatgac ccaacttaca
      181 ctaccaatag aaacttctgt tagaattcct caaaatgata atacgcgata tgttaatgaa
      241 attgttgaaa cgatatctga tagcgaattc gatgaattta tatgtcctaa taacaaaaga
      301 ataggtttta aaagatatgc ataccgtaat gatagatata gttttaaacg tgacttcaag
      361 ctatatgaat gtgatgactg ttcatcatgt tctttgagac atcaatgcat gaagccaaat
      421 tcgaaatcca ataagaaaat tatgaagaat tataattggg aatactttaa agcccaaatt
      481 aatcaaaagc tttctgaacc agaaac
//
LOCUS       DAKTIO010000391          493 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.417, whole genome shotgun sequence.
ACCESSION   DAKTIO010000391 DAKTIO010000000
VERSION     DAKTIO010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.417"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..>493)
                     /locus_tag="O0293_002951"
     rRNA            complement(<1..>493)
                     /locus_tag="O0293_002951"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          105 a          148 c          102 g          138 t
ORIGIN      
        1 tacacatgga attccacttt cctcttctgc actcaagttt tccagtttcc aatgaccctc
       61 cacggttgag ccgtgggctt tcacatcaga cttaaaaaac cgcctacgcg cgctttacgc
      121 ccaataattc cggataacgc ttgccaccta cgtattaccg cggctgctgg cacgtagtta
      181 gccgtggctt tctgattagg taccgtcaag atgtgcacag ttacttacac atatgttctt
      241 ccctaataac agagttttac gatccgaaga ccttcatcac tcacgcggcg ttgctccgtc
      301 aggctttcgc ccattgcgga agattcccta ctgctgcctc ccgtaggagt ctggaccgtg
      361 tctcagttcc agtgtggccg atcaccctct caggtcggct atgcatcgtt gccttggtaa
      421 gccgttacct taccaactag ctaatgcagc gcggatccat ctataagtga cagcaagacc
      481 gtctttcact ttt
//
LOCUS       DAKTIO010000392          490 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.418, whole genome shotgun sequence.
ACCESSION   DAKTIO010000392 DAKTIO010000000
VERSION     DAKTIO010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.418"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            13..263
                     /locus_tag="O0293_002952"
                     /pseudo
     CDS             13..263
                     /locus_tag="O0293_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187510.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
BASE COUNT          199 a           62 c           68 g          161 t
ORIGIN      
        1 aaaggagtga taatgatggt gaaaaaaaca aaatccaatt cactaaaaaa agttgcaaca
       61 cttgcattag caaatttatt attagttggt gcacttactg acaatagtgc caaagccgaa
      121 tctaagaaag atgatactga tttgaagtta gttagtcata acgtttatat gttatcgacc
      181 gttttgtatc caaactggag acttttaaca taaaattact tatcattcaa aaagtaaaac
      241 agcataatat caaggtttat aactttatca ttatcaataa tacctcatat aaaataaaat
      301 tttagggact ttttagggac tttaaattta aaattacaag tttaatagaa acatcaaaat
      361 aatcacatgt ttgtgtggaa tgtacacccc aaaagctaga ctgaaaaatc tattttttga
      421 ggtgtatttt tataggtaaa tataataaat tagagtagac aactcagaat tccaatttta
      481 taataatatt
//
LOCUS       DAKTIO010000393          490 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.419, whole genome shotgun sequence.
ACCESSION   DAKTIO010000393 DAKTIO010000000
VERSION     DAKTIO010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.419"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            117..>490
                     /locus_tag="O0293_002953"
     CDS             117..>490
                     /locus_tag="O0293_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HCY0281749.1"
                     /translation="MTTEMNDVTNHQPNRKSNDENIMAMLIYLLSLFTSIIGPLIIWL
                     LKKDESKLVDRAGKNYLNYTISYIIWSIVLVVITLIGVFLIATDIDFIIIIGFIITFI
                     GILSIFAFSILSFVFTIIACVK"
BASE COUNT          155 a           69 c           66 g          200 t
ORIGIN      
        1 cgccgacgtc caccaattat ttttattttt aataattgtt taataaacat gtttattttt
       61 gattatattt tgttatagtt aaagataata ttacaatttt caaaggagtg tctaatatga
      121 caactgaaat gaacgacgta acaaatcatc aaccgaatcg caaatccaat gatgagaaca
      181 ttatggcgat gcttatctat ttattaagtt tattcacatc tattattggt ccactgataa
      241 tatggttatt gaaaaaagat gaatcgaaac tagttgatcg tgctggaaaa aattatctta
      301 actatacgat ttcttatatt atttggtcta tcgttttagt cgttatcact ttaataggcg
      361 ttttcctaat tgctacagat attgatttca ttattattat aggtttcatc attacgttta
      421 tcggtatttt atccatattc gctttctcaa tattatcttt tgtattcaca attattgcgt
      481 gtgtcaaata
//
LOCUS       DAKTIO010000394          481 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.421, whole genome shotgun sequence.
ACCESSION   DAKTIO010000394 DAKTIO010000000
VERSION     DAKTIO010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.421"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..161
                     /locus_tag="O0293_002954"
     CDS             <1..161
                     /locus_tag="O0293_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0281750.1"
                     /translation="IMALSMLLTLNPQNIIGFIGWLVMTAGFFLLNMSSIIDKKIYVL
                     SKTNTVEK"
     gene            127..372
                     /locus_tag="O0293_002955"
     CDS             127..372
                     /locus_tag="O0293_002955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281751.1"
                     /translation="MYYLKLTRWKNDGLAGFTAGSISAILVYWTNQKNEFGIKDKNDW
                     IGHKLDVGIDAVEKSAEKTVDGVENVMVKLQKVFLII"
BASE COUNT          186 a           57 c           87 g          151 t
ORIGIN      
        1 caataatggc tctaagtatg ttattaactt taaatcctca aaatataata ggctttattg
       61 gttggttggt aatgactgca ggtttctttc tgttaaacat gtcatcgatt attgacaaaa
      121 aaatttatgt attatctaaa actaacacgg tggaaaaatg atggtttagc tggatttact
      181 gcaggttcta tttcggcaat acttgtatat tggaccaatc aaaaaaatga atttggaata
      241 aaagataaaa acgattggat aggacataaa ctagacgttg gtatagatgc tgtagaaaaa
      301 tctgcagaaa aaacagtaga tggtgttgaa aatgtcatgg tgaagcttca aaaagtattt
      361 ctaatcatat aagccctaag aaatggagct ggtaaatgtt gctatgcgaa tctaaaatca
      421 tcaataaaaa cccaaaatat agaattatta aatataatga tgaatactta atggtcgata
      481 t
//
LOCUS       DAKTIO010000395          476 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.422, whole genome shotgun sequence.
ACCESSION   DAKTIO010000395 DAKTIO010000000
VERSION     DAKTIO010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.422"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            65..>476
                     /locus_tag="O0293_002956"
     CDS             65..>476
                     /locus_tag="O0293_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281752.1"
                     /translation="MKSTAQLTKENNVKSLRLSNTDREIFENYMTYMRSDFRVNPHDT
                     ELIINRILKQLLSAEQHGLLALDFFNHDPKAHAIKELKAMPNETFKNIFKYIYQHIVL
                     LIGIVSFLKGFLGFFMEKNGSNLYFVSFPFSVVVG"
BASE COUNT          178 a           70 c           67 g          161 t
ORIGIN      
        1 tactataaaa tcatttccat tttaatttgg aattaaacat ttttaaagga gcgcttaagt
       61 aacaatgaaa agtactgcgc aattaacaaa agaaaataat gttaaatcat taagattaag
      121 caatactgat agagaaatct tcgagaatta tatgacttat atgcgctctg actttcgcgt
      181 caaccctcat gatacagagt taattattaa ccgtatactc aaacaattat taagcgcaga
      241 acaacatggt ttattagcct tagatttttt caatcacgac ccaaaagcac atgcaataaa
      301 agaactaaaa gcgatgccta atgaaacatt taaaaatatt tttaaatata tctatcaaca
      361 catcgttcta ttaattggga ttgttagttt tctcaaagga tttttaggat ttttcatgga
      421 aaaaaatgga agtaatttat attttgtttc attcccgttt tcagtagtcg ttggat
//
LOCUS       DAKTIO010000396          469 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.423, whole genome shotgun sequence.
ACCESSION   DAKTIO010000396 DAKTIO010000000
VERSION     DAKTIO010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.423"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..>469
                     /locus_tag="O0293_002957"
     CDS             <1..>469
                     /locus_tag="O0293_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCY0281753.1"
                     /translation="SILIIIGAITFFLLEQFNTMQHMGLVEKIGNSFFQSVTTRTAGF
                     NSIDIASINKSTALMLMLLMFIGGAPLSAAGGIKITTFAVAFIFVLNYIRKENNVSVF
                     NKEISDKHIKLSIVTINISFLFISIITFILSIINPNISLIKLLFEVVSAFGTVG"
BASE COUNT          163 a           72 c           66 g          168 t
ORIGIN      
        1 ctagtatcct aataattata ggagctatta cattcttttt attagaacag tttaatacta
       61 tgcaacatat gggactagtt gaaaaaatcg gaaattcttt tttccaatca gtaacaacac
      121 gaacagcggg ttttaacagt atagatatag caagcattaa caaatctacc gcattaatgt
      181 taatgctact tatgtttatt ggtggtgccc ctctcagtgc agctggagga attaaaataa
      241 ctacttttgc agttgcgttt atttttgtac taaattatat acgtaaagaa aataatgttt
      301 cagtattcaa taaagaaata tctgacaaac atataaaact atctattgtt accattaata
      361 tctcatttct atttatcagc atcattactt ttatattatc gataattaat ccgaacatat
      421 cattaatcaa gttattattc gaagtggttt ctgcattcgg aacagtagg
//
LOCUS       DAKTIO010000397          465 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.424, whole genome shotgun sequence.
ACCESSION   DAKTIO010000397 DAKTIO010000000
VERSION     DAKTIO010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.424"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..244)
                     /locus_tag="O0293_002958"
     CDS             complement(<1..244)
                     /locus_tag="O0293_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCY0281754.1"
                     /translation="MSSDLKQTPLYQNYVDRGAKIVEFGGWAMPVQFSSIKEEHNAVR
                     YEIGLFDVSHMGEIEVTGKDASQFVQYLLSNDTDNLT"
     regulatory      complement(319..416)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(403..>465)
                     /locus_tag="O0293_002959"
     CDS             complement(403..>465)
                     /locus_tag="O0293_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HCY0281755.1"
                     /translation="LSISEIYYELLNLIKASDQY"
BASE COUNT          151 a           98 c           56 g          160 t
ORIGIN      
        1 tagttaaatt atcagtatca tttgataata aatattgcac aaactgacta gcatctttac
       61 ctgttacttc aatttcaccc atatgactaa catcaaacag gccaatttcg tatcgaacag
      121 cattatgctc ctctttaata cttgaaaatt gaacaggcat cgcccatcct ccgaattcca
      181 caatttttgc acctctatca acataatttt gatataaagg tgtttgtttt aaatcacttg
      241 acattgaaat acccccttaa aattataaaa acaggatagc aatcgctacc ctgtttataa
      301 atcaccaatt caggaacgcg ttacagtatt atccttttgc ctgagagttt cgtaataaat
      361 tacttgcacc ttcggcgata gagtcttaac gctctattct ctctaatact gatcactcgc
      421 ttttattaaa tttagcaatt cataatatat ttctgaaatt gatag
//
LOCUS       DAKTIO010000398          461 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.425, whole genome shotgun sequence.
ACCESSION   DAKTIO010000398 DAKTIO010000000
VERSION     DAKTIO010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.425"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(84..191)
                     /locus_tag="O0293_002960"
                     /pseudo
     CDS             complement(84..191)
                     /locus_tag="O0293_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649608.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
BASE COUNT          175 a           70 c           49 g          167 t
ORIGIN      
        1 gactcagttt ttaatattta caactaaagc gaaaaattat tttgaacact ttatgaaatt
       61 tttatttcaa acttctatta accctattca gtaggtttga aattaatact atcagaaaaa
      121 tacacatttt cttctttatt tcttataaag ttaaattcta gttctttaga acctacggat
      181 gagcctttta atcgccatca ccttcataca gtttgaagtc atatttacca attgtaaatg
      241 acgactagat gatatatagt tcaagaaaca tcaaaattcg ttaaaacata atcaaatttt
      301 attatgttca aaactttatt ttccaaaaaa taaaaaagaa gttaaacaaa gattggttgc
      361 ttaacttcca ttattgaatt cattagtccg atttgacata gatcccgaaa aataatggct
      421 aatagccact tatagtcgaa attatgaact atataattca t
//
LOCUS       DAKTIO010000399          439 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.426, whole genome shotgun sequence.
ACCESSION   DAKTIO010000399 DAKTIO010000000
VERSION     DAKTIO010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.426"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..>439
                     /locus_tag="O0293_002961"
     CDS             <1..>439
                     /locus_tag="O0293_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0281756.1"
                     /translation="KPKTVSSNNKSNSNSSTLNYLKTLENRGWDFDGSYGWQCFDLVN
                     VYWNHLYGHGLKGYGAKDIPYANNFNSEAKIYHNTPTFKAEPGDLVVFSGRFGGGYGH
                     TAIVLNGDYDGKLMKFQSLDQNWNNGGWRKAEVAHKVVHNYEND"
BASE COUNT          165 a           45 c           94 g          135 t
ORIGIN      
        1 aaaccaaaaa cagtgtcttc aaataataaa agtaatagta atagcagtac attaaattat
       61 ttgaaaacat tagagaatag aggatgggat ttcgacggta gttatggatg gcaatgtttc
      121 gatttagtta atgtatattg gaatcatctt tatggtcatg gattaaaagg atatggagct
      181 aaagatatac catatgcaaa taattttaat agtgaagcta aaatttatca caacacacca
      241 actttcaaag ctgaacctgg ggacttagtg gtttttagtg gaagatttgg tggaggatat
      301 ggtcatacag ctattgtctt aaatggtgat tatgatggaa aattaatgaa gttccaaagt
      361 ttagatcaaa actggaataa tggtggatgg cgtaaagcag aggttgcaca taaagttgtt
      421 cataattatg aaaatgata
//
LOCUS       DAKTIO010000400          436 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.427, whole genome shotgun sequence.
ACCESSION   DAKTIO010000400 DAKTIO010000000
VERSION     DAKTIO010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.427"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(92..>436)
                     /locus_tag="O0293_002962"
     CDS             complement(92..>436)
                     /locus_tag="O0293_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HCY0281757.1"
                     /translation="RYSATTIAKDLKELNIYRVPIDCETWIYKAINNQTEQEMREKFR
                     HYCEHEVLSSIINGSYIIVKTSPGFAQGINYFIDQLNIEEILGTVSGNDTTLILTASN
                     DMAEYVYAKLFK"
BASE COUNT          134 a           73 c           65 g          164 t
ORIGIN      
        1 cataaaaaaa cgcgtacaca agttaatatt tggtaactaa cttgagtaca cgtgatacga
       61 aacaaatttt tattattcat ttgatacatg tctatttaaa taattttgca tatacgtatt
      121 ctgccatatc atttgaggca gttaagatta atgtagtgtc atttccactc accgtaccta
      181 atatctcttc tatatttagc tgatcgataa aatagtttat gccttgggcg aaaccaggtg
      241 aggttttgac gataatgtat gaaccattga tgattgaact tagaacttca tgttcacaat
      301 agtgtctaaa cttttctctc atctcttgtt ctgtttgatt attaatagct ttataaatcc
      361 atgtctcaca atcgataggg acacgatata tatttagttc cttcaagtct ttagcaattg
      421 ttgttgcact atatct
//
LOCUS       DAKTIO010000401          425 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.428, whole genome shotgun sequence.
ACCESSION   DAKTIO010000401 DAKTIO010000000
VERSION     DAKTIO010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.428"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            121..>425
                     /locus_tag="O0293_002963"
     CDS             121..>425
                     /locus_tag="O0293_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCY0281758.1"
                     /translation="MYKNYNMTPTYTTNRNFVRIPQNDISRYVNEIVETIPDSEFDEF
                     RHHRGATSYHPKMMLKIILYAYTQSVFSGRRIEKLLHDSIRMMWLAQDQTLSYKTINR
                     "
BASE COUNT          159 a           72 c           57 g          137 t
ORIGIN      
        1 aaaataagac atttttccca acttgcacat aaatgtaaaa ttcaataaaa tgaattttct
       61 gtgttgggtc ccttcgtata atttaataaa tactactaaa ctaaattaaa gaggtgcctt
      121 atgtataaaa attataacat gaccccaact tacactacca atagaaactt cgttagaatt
      181 cctcaaaatg atatttcgcg atatgttaat gaaattgttg aaacgatacc tgatagcgaa
      241 ttcgatgaat tcagacatca tcgtggtgca acatcctatc atccaaaaat gatgttaaaa
      301 atcatcttat atgcatatac tcaatctgta ttttctggtc gtagaataga gaaattactt
      361 catgacagta ttcgaatgat gtggttagcc caagatcaaa cactttctta taaaactatt
      421 aatcg
//
LOCUS       DAKTIO010000402          422 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.429, whole genome shotgun sequence.
ACCESSION   DAKTIO010000402 DAKTIO010000000
VERSION     DAKTIO010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.429"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            2..>422
                     /locus_tag="O0293_002964"
     CDS             2..>422
                     /locus_tag="O0293_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HCY0281759.1"
                     /translation="MKKIFMMVHELDVNKGGMTSSMFNRSKEFYDADIPADIVTFDYK
                     GNYDEIIKALKKQGKMDRRTKMYNVFEYFKQISNNKHFKSNKLLYKHISERLKNTIEI
                     EESKGISRYFDITTGTYIAYIRKSKSEKVIDFFKDNKR"
BASE COUNT          181 a           37 c           71 g          133 t
ORIGIN      
        1 aatgaaaaaa atatttatga tggtacatga gttagatgtg aataaaggtg gtatgacctc
       61 ttcgatgttc aatagaagta aagagtttta tgatgcggac atacctgctg atattgttac
      121 tttcgattac aaaggaaact atgatgaaat tattaaagct ttgaaaaaac aaggtaaaat
      181 ggatcgaaga acgaaaatgt ataatgtatt tgagtatttt aaacaaattt caaataataa
      241 acattttaag tctaataaat tgttatataa acatatttca gaaagactaa aaaatacgat
      301 tgaaattgaa gagagtaaag gtatttcaag atattttgat ataacgactg gtacatatat
      361 tgcctacatt agaaaaagta aatctgaaaa agtgattgat ttctttaaag ataataaacg
      421 aa
//
LOCUS       DAKTIO010000403          412 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.430, whole genome shotgun sequence.
ACCESSION   DAKTIO010000403 DAKTIO010000000
VERSION     DAKTIO010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.430"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            148..>412
                     /locus_tag="O0293_002965"
     CDS             148..>412
                     /locus_tag="O0293_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0281760.1"
                     /translation="MLCETENINKNPKYRIIKYKDEYLMIDLVSTWLALFFPMINWLI
                     PKKYVKISEKDFETLNIVKTAKINSFWPVAGSTVLFGVMLRRYS"
BASE COUNT          158 a           52 c           69 g          133 t
ORIGIN      
        1 cgcgttataa tgaaaaataa ttgattacat ttaaaatatt ctaaatgttg tcgacacaat
       61 ccttttaaga cgctagtaga atttaaatga cttctaatgt atatgaaagt gtatcaatat
      121 aaaaccaatt gaaaagaagt ggagacattg ctttgtgaaa ctgaaaatat taataagaat
      181 cccaaatata gaattatcaa atacaaagat gaatatttga tgattgattt agtaagtaca
      241 tggttagcac tctttttccc aatgattaat tggctgattc caaaaaagta cgtcaaaatc
      301 agcgaaaaag attttgaaac tttaaacatt gtgaagacag ctaaaatcaa ttctttttgg
      361 ccagtggcag gaagtacggt cttatttggt gttatgttaa gaaggtattc cc
//
LOCUS       DAKTIO010000404          408 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.431, whole genome shotgun sequence.
ACCESSION   DAKTIO010000404 DAKTIO010000000
VERSION     DAKTIO010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.431"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
BASE COUNT          137 a           75 c           42 g          154 t
ORIGIN      
        1 atatttatgt ctaaagataa tctcaatgtt gttcacgtca ataaaattat ccctaggttt
       61 ttaaaaattg tacatgttta aacaatcaaa agtgtacatt attaaattat catttccagt
      121 tagatttaga aaacattcac accacgcatg gaccaacgta ttcgtcttca ttcattttag
      181 tctgatgcca actcaccaaa tgatttcccc ttttatcagt taacacatat ttttaaactc
      241 atccttcttc aaccttatac tttaaacttt ttaccactat aaaaaagaag atataactcg
      301 tatactggtc agataaggtc gatgccttaa tgccatatac atgttatatc ttctatatta
      361 attattgaac aataacaccc ttcgtattgt aaggtttatt ttgtttta
//
LOCUS       DAKTIO010000405          400 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.432, whole genome shotgun sequence.
ACCESSION   DAKTIO010000405 DAKTIO010000000
VERSION     DAKTIO010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.432"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..378)
                     /gene="sarU"
                     /locus_tag="O0293_002966"
     CDS             complement(<1..378)
                     /gene="sarU"
                     /locus_tag="O0293_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarU"
                     /protein_id="HCY0281761.1"
                     /translation="MDYQTFEKVNKFINVEAYIFFLTQELKQQYKLSLKELLILAYFY
                     YKNEHSISLKEIIGDILYKQSDVVKNIKSLSKKGFINKSRNEADERRIFVSVTPIQRK
                     KIACVINELDKIIKGFNKERDYIK"
BASE COUNT          121 a           61 c           46 g          172 t
ORIGIN      
        1 ttttatgtag tctctttcct tattaaatcc tttaattatt ttatctaact cattaataac
       61 acaagcaatc tttttacgtt gtattggagt aactgaaaca aaaatacggc gttcatctgc
      121 ttcgtttcta gacttattta taaatccttt tttagatagt gacttaatgt tctttacaac
      181 atcagattgt ttgtaaagta tgtcaccaat gatttctttt agtgaaatac tgtgttcatt
      241 tttgtaataa aaatatgcta atatcaacaa ttcttttaat gataatttat attgttgctt
      301 taactcttga gtgagaaaaa atatgtacgc ttctacattt atgaatttat tgaccttttc
      361 gaaagtttga tagtccacct tacattctcc tactattttc
//
LOCUS       DAKTIO010000406          384 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.433, whole genome shotgun sequence.
ACCESSION   DAKTIO010000406 DAKTIO010000000
VERSION     DAKTIO010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.433"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
BASE COUNT          119 a           61 c           70 g          134 t
ORIGIN      
        1 gcagaggcca tatatctggc tgttggtctc acaggagaca tcttccttgt catcactcat
       61 atacatatat cttgataaca tagagttgtt atagtcgcta cgccactcat actagttact
      121 gggtggttgt ttttatgtta tattataaat gatcaaacca caccacctat taatttagga
      181 gtgtggttgt tttcaagggc aaaaaaaggg cataattttg aaacaaaggg caagggtatg
      241 aataataaat tatcttgaaa attttataaa tgcatatata acaatgtttt gattgccctt
      301 gaacttcctt gataatctgg ctaattattc ctgctaaata aatcatatta atggctcagt
      361 aacgtgatgt tgctgggctt ttta
//
LOCUS       DAKTIO010000407          383 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.435, whole genome shotgun sequence.
ACCESSION   DAKTIO010000407 DAKTIO010000000
VERSION     DAKTIO010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.435"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(109..>383)
                     /gene="isaB"
                     /locus_tag="O0293_002967"
     CDS             complement(109..>383)
                     /gene="isaB"
                     /locus_tag="O0293_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="immunodominant staphylococcal antigen IsaB"
                     /protein_id="HCY0281762.1"
                     /translation="EKYDQTFKGVSAKGNEASQLQFVVKNNISLKDIQKAYGKDLKKE
                     NGKTKEADSGIFYYQNAKKTLGIWFVVDHNRVVEVTVGHTPYKTSK"
BASE COUNT          114 a           71 c           44 g          154 t
ORIGIN      
        1 tttatcaatt tcaaacaact atgtaagacg caataatcgt tattatttaa taaaaaagac
       61 tagaatgcgg tatcactcct cacactctag ccttaatatg ccattatttt atttacttgt
      121 tttgtatggt gtatgtccaa ctgttacttc gacaactcta ttatgatcaa cgacaaacca
      181 gatgcctaat gtctttttag cattttgata gtaaaaaata ccgctatcag cttcctttgt
      241 tttaccattt tcttttttca agtccttgcc ataagctttt tggatatctt ttaatgaaat
      301 attattttta actacaaatt gcaattgact tgcttcgttt ccttttgcag aaacaccttt
      361 aaaagtttga tcatatttct cta
//
LOCUS       DAKTIO010000408          367 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.437, whole genome shotgun sequence.
ACCESSION   DAKTIO010000408 DAKTIO010000000
VERSION     DAKTIO010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.437"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(195..367)
                     /locus_tag="O0293_002968"
                     /pseudo
     CDS             complement(195..367)
                     /locus_tag="O0293_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT          119 a           58 c           39 g          151 t
ORIGIN      
        1 aaattaatta gttagcctta aatttaacaa attaaatcaa accgaatgag gctaaattgg
       61 ttatttttca agatatgatt tgtataaatc aaaacatttc ttacaaatca aatgtgtata
      121 aaatgtgata tacattttca accttaaaaa agttctaaaa aaagatgagc atctactgtc
      181 tcatctttca gttctttttt acgggtctgt tttctaattt gagcacaatc ttcgatttct
      241 ttatctaaat gactaccaat taaatctatt tcttctattg ttaaatcgct atctccatct
      301 tcttttatct ctggtattat tttttcttca actaagtcac gatatagtgt ttttgaattc
      361 tcgttca
//
LOCUS       DAKTIO010000409          365 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.438, whole genome shotgun sequence.
ACCESSION   DAKTIO010000409 DAKTIO010000000
VERSION     DAKTIO010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.438"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(238..352)
                     /gene="rrf"
                     /locus_tag="O0293_002969"
     rRNA            complement(238..352)
                     /gene="rrf"
                     /locus_tag="O0293_002969"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          100 a           74 c           62 g          129 t
ORIGIN      
        1 taagatgtta tattcattgt taatttaaca catagtaaga aaaacagtca taaattgatt
       61 tctaattgaa atcatcttat gactgctttt tattatactt tacatttctc gtttcgtcag
      121 attcaaacgt tttcacttcg ccaagccatc tttctttgtg tttgctttta ttttgacgtt
      181 ttagacataa aaaaagagac cttgcggtct caatgcggct catcgcatcc actttttgcc
      241 tggcaacgtt ctactctagc ggaacgtaag ttcgactacc atcgacgcta aggagcttaa
      301 cttctgtgtt cggcatggga acaggtgtga cctccttgct atagtcacca gacatatgaa
      361 tgtaa
//
LOCUS       DAKTIO010000410          348 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.439, whole genome shotgun sequence.
ACCESSION   DAKTIO010000410 DAKTIO010000000
VERSION     DAKTIO010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.439"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
BASE COUNT          110 a           65 c           46 g          127 t
ORIGIN      
        1 taataataaa actagatacc atcgtactct atcatggctt tcttataatc gagtagaagc
       61 atcatcatta cttgattatt tgctctttac aacaccgagc gtgcccgtac tcggtaattc
      121 aataccttgc gtaacccgtc actgtgagtt gggttaatga taataaagcc cacacctttt
      181 aaaaagatgt gggtaattta tataattttt atttacattt ttaacttata aaaaaagcgc
      241 ctatgtcatg atttaccatc acataggcgc ttatcaataa attattactt attactttcc
      301 atttcatcta atttatgcgg atttcctgta attagatgac aacttatt
//
LOCUS       DAKTIO010000411          346 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.440, whole genome shotgun sequence.
ACCESSION   DAKTIO010000411 DAKTIO010000000
VERSION     DAKTIO010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.440"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..241
                     /locus_tag="O0293_002970"
     CDS             <1..241
                     /locus_tag="O0293_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281763.1"
                     /translation="TIQLQCFNNSNWIWIFTYLAIILLIVGFFYVFFIPQSLLAFGPY
                     IQVSNWVFIIFSFIVMPIGLRIERNISKKHSHTFL"
BASE COUNT          122 a           50 c           40 g          134 t
ORIGIN      
        1 aacaatacaa ctgcagtgtt ttaacaattc aaattggata tggatattca cctatttagc
       61 aatcattcta ctaattgtag gatttttcta tgtattcttt attccacaat cattacttgc
      121 ctttggacca tatattcagg taagcaattg ggtatttatt atattttcat ttatagttat
      181 gcctattgga ttacgcattg aaagaaatat ttcgaaaaaa cattctcata catttttata
      241 aacaaaaagc ttattacatc atatgcgaat ttggtactgt taatgataaa atcactaaat
      301 tacagttacc gaaaaacata ttttgcaata agctttttat taatta
//
LOCUS       DAKTIO010000412          342 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.441, whole genome shotgun sequence.
ACCESSION   DAKTIO010000412 DAKTIO010000000
VERSION     DAKTIO010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.441"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..223
                     /locus_tag="O0293_002971"
     CDS             <1..223
                     /locus_tag="O0293_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0281764.1"
                     /translation="LEPWTSCEFDFTRDGKLNVSFDYIDWINTEFDQLGRQNYYMYKK
                     FGVIPEMEYEMEEVKEIEQYIKEQEEAEQ"
     gene            234..>342
                     /locus_tag="O0293_002972"
     CDS             234..>342
                     /locus_tag="O0293_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF600 family protein"
                     /protein_id="HCY0281765.1"
                     /translation="MTFEEKISKLYNEIANEISSMIPVEWEKVYTMAYID"
BASE COUNT          141 a           33 c           75 g           93 t
ORIGIN      
        1 gcttgaacca tggacatcat gcgaatttga ctttacaaga gatggcaaat tgaatgtatc
       61 ttttgattat atagattgga taaatacaga gtttgatcaa ttgggccgtc aaaattatta
      121 tatgtacaaa aaatttgggg ttataccaga aatggaatat gaaatggaag aagttaaaga
      181 aatcgaacaa tatattaaag agcaagaaga agctgaacaa tagaggtgat aacatgactt
      241 tcgaagagaa aataagcaaa ttatataatg agattgcgaa tgagattagc agtatgatac
      301 cggtagagtg ggaaaaagta tatacaatgg cttatataga tg
//
LOCUS       DAKTIO010000413          341 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.442, whole genome shotgun sequence.
ACCESSION   DAKTIO010000413 DAKTIO010000000
VERSION     DAKTIO010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.442"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..142
                     /locus_tag="O0293_002973"
     CDS             <1..142
                     /locus_tag="O0293_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281766.1"
                     /translation="EKGSITLSKEAAIIFAIAKFKPFKNRIKNNPQKTNPFLKLHENK
                     KS"
BASE COUNT          145 a           70 c           32 g           94 t
ORIGIN      
        1 agaaaaaggt tctattactt tgtcaaaaga agcagctatc atatttgcaa tcgcaaaatt
       61 caaaccattt aagaacagaa ttaaaaataa cccacaaaaa acaaatccat ttcttaaatt
      121 acatgaaaac aaaaaatctt aatcacttta tttatagcat ttctaatctc agaaatgcta
      181 taaaggacaa cgccctcttt cataactata taagtcaaga aaccaagaca tttcaaacat
      241 ctttaacccc ttcaatagca cattccctaa aacataaagc aataacaatg aacgatcaaa
      301 attccttacg tacttatgat gtgtcagtga atcgtctaca c
//
LOCUS       DAKTIO010000414          340 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.443, whole genome shotgun sequence.
ACCESSION   DAKTIO010000414 DAKTIO010000000
VERSION     DAKTIO010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.443"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            108..190
                     /locus_tag="O0293_002974"
     tRNA            108..190
                     /locus_tag="O0293_002974"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:141..143,aa:Leu,seq:gag)
     gene            213..286
                     /locus_tag="O0293_002975"
     tRNA            213..286
                     /locus_tag="O0293_002975"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:245..247,aa:Gly,seq:tcc)
BASE COUNT          115 a           59 c           68 g           98 t
ORIGIN      
        1 aattaattta atgtaaaaaa acgattaaaa gttattgaca aatattttta aacataatat
       61 aataaataac gttctgaaaa acgaagatgt tttaaatgcc tatatccgcg gtcgtggcgg
      121 aatggcagac gcgctaggtt gagggcctag tgggagaaat cccgtggagg ttcaagtcct
      181 ctcggccgca tcaaaattct taatttaaat aagcgggtgt agtttaatgg caaaacctca
      241 gccttccaag ctgatgttgt gggttcgatt cccatcaccc gctccataga taattttaat
      301 gaacattgaa aactgaatga caatatgtca acgttaattc
//
LOCUS       DAKTIO010000415          331 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.446, whole genome shotgun sequence.
ACCESSION   DAKTIO010000415 DAKTIO010000000
VERSION     DAKTIO010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.446"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            20..>331
                     /locus_tag="O0293_002976"
     CDS             20..>331
                     /locus_tag="O0293_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCY0281767.1"
                     /translation="MLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDL
                     IRLSKTFLIESRDLTTVDFEFFSRDILDDFKGVDRNDIEIRQHRIILHKGEEMIEYKL
                     IN"
BASE COUNT          127 a           42 c           51 g          111 t
ORIGIN      
        1 caaagtgacc aacaaatttg tgctatttca aaaaatacca cttcttatca aaagacatgt
       61 atacagtatt aatgtcaaag ctttttcgct cattgaaatg ttagtagcga tgatggttat
      121 aagtataact ttactaattg ttccagactt aattagactt agtaaaactt ttctaattga
      181 aagtagggat ttaacaactg tagatttcga atttttctca agagatattc tagatgattt
      241 taaaggagta gatagaaacg atattgaaat taggcaacac cgtatcattt tacataaagg
      301 tgaagaaatg atcgaataca aattaattaa t
//
LOCUS       DAKTIO010000416          320 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.447, whole genome shotgun sequence.
ACCESSION   DAKTIO010000416 DAKTIO010000000
VERSION     DAKTIO010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.447"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            35..>320
                     /gene="sarX"
                     /locus_tag="O0293_002977"
     CDS             35..>320
                     /gene="sarX"
                     /locus_tag="O0293_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarX"
                     /protein_id="HCY0281768.1"
                     /translation="MNTEKLETLLGFYKQYKALSEYIDKKYKLSLNDLAVLDLTMKHC
                     KDEKVLMQSFLKTAMDELDLSRTKLLVSIRRLIEKERLSKVRSSKDERKIY"
BASE COUNT          130 a           32 c           55 g          103 t
ORIGIN      
        1 agcattgtct ttatatattt gggggtgcaa cattttgaat actgagaaat tagaaacatt
       61 gcttggcttc tataaacaat ataaagcatt atctgaatat attgataaaa aatataagtt
      121 gtcgctaaat gatttagcag tcttagattt aacgatgaag cattgcaaag atgaaaaagt
      181 acttatgcaa tcatttttaa aaactgcaat ggatgagcta gatttaagta ggacaaaatt
      241 attagtttct ataagaagac taattgaaaa agaaagactt agtaaagtta gatcatctaa
      301 agatgagcgt aaaatttata
//
LOCUS       DAKTIO010000417          315 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.449, whole genome shotgun sequence.
ACCESSION   DAKTIO010000417 DAKTIO010000000
VERSION     DAKTIO010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.449"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..>315)
                     /locus_tag="O0293_002978"
     CDS             complement(<1..>315)
                     /locus_tag="O0293_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCY0281769.1"
                     /translation="FALGITSGRYSYSLTDYTVQIFNYLMLAIPSFIAGVFAIFIFSF
                     ELQWFPFQGSVDINLKEGTFEYYMSKIYHTFLPAFTLGLLSTAGYIQYLRNDIIENSK
                     KD"
BASE COUNT          135 a           46 c           39 g           95 t
ORIGIN      
        1 taatcttttt tagaattttc aataatatca ttacgtaaat attgaatata accagcagta
       61 gataataaac ctaaagtgaa tgcaggtaaa aatgtgtgat atattttact catataatat
      121 tcaaaagtac cttctttaag gttaatatca acagaacctt gaaacgggaa ccattgtaat
      181 tcaaaagaaa aaataaaaat tgcaaatact cccgcaataa aagatggaat ggctaacatc
      241 aaataattaa atatttgcac agtataatcc gtcaaactgt aagaatatct acctgacgtt
      301 attcctaaag caaat
//
LOCUS       DAKTIO010000418          303 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.450, whole genome shotgun sequence.
ACCESSION   DAKTIO010000418 DAKTIO010000000
VERSION     DAKTIO010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.450"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
BASE COUNT          120 a           36 c           48 g           99 t
ORIGIN      
        1 aattaataat aattgtattt aaatgcaatg ctgttgttct ttcatactta caagcaagtc
       61 ataagaaatg agaaattaaa ttcttatatg atgaataaat gatgactgca gaagaaattg
      121 ctaaaaaagg agtggagtga ggcattctac tgttcatatt gttaacaaat atcaaaatga
      181 taggaacttg aagaatctaa gactgttaaa atcgagacaa taagaaactc gttccaaatt
      241 tgaatcctat aatgaattcg tctcatgaag aagttattta cttttcatta gactgattta
      301 cta
//
LOCUS       DAKTIO010000419          300 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.451, whole genome shotgun sequence.
ACCESSION   DAKTIO010000419 DAKTIO010000000
VERSION     DAKTIO010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.451"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            18..161
                     /gene="rpmG"
                     /locus_tag="O0293_002979"
     CDS             18..161
                     /gene="rpmG"
                     /locus_tag="O0293_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002451211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCY0281770.1"
                     /translation="MRKIPLNCEACGNRNYNVPKQEGSATRLTLKKYCPKCNAHTIHK
                     ESK"
     gene            217..>300
                     /gene="secE"
                     /locus_tag="O0293_002980"
     CDS             217..>300
                     /gene="secE"
                     /locus_tag="O0293_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCY0281771.1"
                     /translation="MAKKESFFKGVKSEMEKTSWPTKEELFK"
BASE COUNT          127 a           36 c           59 g           78 t
ORIGIN      
        1 aataaaggtg aattattgtg agaaaaatac ctttaaattg tgaagcttgt ggcaatagaa
       61 attataatgt tcctaagcaa gaaggctcgg caacaagatt aaccttaaag aaatattgtc
      121 caaaatgtaa cgcgcacaca attcataaag aatcgaaata aatacattcg aaataatact
      181 ttgataatat gttcaaagga tttggaggtt gagcagatgg ctaaaaaaga aagtttcttt
      241 aaaggcgtta agtctgaaat ggaaaaaaca agttggccga cgaaagaaga gctatttaaa
//
LOCUS       DAKTIO010000420          299 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.452, whole genome shotgun sequence.
ACCESSION   DAKTIO010000420 DAKTIO010000000
VERSION     DAKTIO010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.452"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(139..227)
                     /locus_tag="O0293_002981"
     tRNA            complement(139..227)
                     /locus_tag="O0293_002981"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(190..192),aa:Ser,seq:gct)
BASE COUNT          100 a           66 c           57 g           76 t
ORIGIN      
        1 aaaaaccttt cttagagaag attatctagg aaaggttttt agaattttaa aattgatata
       61 aaagaacagt gaaattctca ttcattatat ataagtaaac aaaaaagcta aaatggtata
      121 taccatttta gcaagtaacg gaaacggagg gattcgaacc ctcgcgccgc tttcgcgacc
      181 tacaccctta gcaggggcgc ctcttcagcc aacttgagta cgtttccaat ggctccacag
      241 gtaggactcg aacctacgac cgatcggtta acagccgata gctctaccac tgagctact
//
LOCUS       DAKTIO010000421          299 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.453, whole genome shotgun sequence.
ACCESSION   DAKTIO010000421 DAKTIO010000000
VERSION     DAKTIO010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.453"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            147..>299
                     /locus_tag="O0293_002982"
     CDS             147..>299
                     /locus_tag="O0293_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCY0281772.1"
                     /translation="MDDKQHTSSSDDERAEIATSNQDQETNSSKRVHLKRWQFISILI
                     GTILITA"
BASE COUNT          122 a           48 c           43 g           86 t
ORIGIN      
        1 ttatcctggt tttaatttaa aataaactaa gttgtgacta aaaattaatc aattatagtg
       61 aaatatggtg cgctatcttg cataaattga tatgattaac tacacagaat ttaaaagtac
      121 ataatacata ataaggaagt gatacaatgg atgataagca acacacatct tcatccgatg
      181 atgaacgcgc tgaaattgca acaagcaatc aagaccaaga aactaattca tcgaaacgcg
      241 ttcacttaaa acgttggcaa ttcatatcaa tattaattgg tacaatccta ataacagct
//
LOCUS       DAKTIO010000422          285 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.455, whole genome shotgun sequence.
ACCESSION   DAKTIO010000422 DAKTIO010000000
VERSION     DAKTIO010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.455"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..>285
                     /locus_tag="O0293_002983"
     CDS             <1..>285
                     /locus_tag="O0293_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281773.1"
                     /translation="GYKKYNEYFTNRDDTTLKITNLYIGQSFGTEGNRNVIDRIGKGH
                     EKLQEIMTYQPENRKTVLIFYKIQPDNIYITNQYGKEAVSRLKRILEQSI"
BASE COUNT          122 a           30 c           49 g           84 t
ORIGIN      
        1 tggggtataa aaaatataat gaatatttta caaacagaga tgatactaca ttaaaaatta
       61 ctaatttata tattgggcaa tcttttggta cagagggtaa tagaaatgtc attgatagaa
      121 tagggaaagg tcatgaaaaa ttacaagaaa ttatgactta tcagccagaa aatagaaaga
      181 ctgtcttaat attttacaag atacagcctg acaatattta tattacaaac caatatggta
      241 aagaggctgt gtctcgatta aaaagaattt tagagcaaag tatac
//
LOCUS       DAKTIO010000423          278 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.456, whole genome shotgun sequence.
ACCESSION   DAKTIO010000423 DAKTIO010000000
VERSION     DAKTIO010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.456"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(24..>278)
                     /locus_tag="O0293_002984"
     rRNA            complement(24..>278)
                     /locus_tag="O0293_002984"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           69 a           76 c           63 g           70 t
ORIGIN      
        1 agatgttccg aatatatcct tagaaaggag gtgatccagc cgcaccttcc gatacggcta
       61 ccttgttacg acttcacccc aatcatttgt cccaccttcg acggctagct cctaaaaggt
      121 tactccaccg gcttcgggtg ttacaaactc tcgtggtgtg acgggcggtg tgtacaagac
      181 ccgggaacgt attcaccgta gcatgctgat ctacgattac tagcgattcc agcttcatgt
      241 agtcgagttg cagactacaa tccgaactga gaacaact
//
LOCUS       DAKTIO010000424          272 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.denovo.458, whole genome shotgun sequence.
ACCESSION   DAKTIO010000424 DAKTIO010000000
VERSION     DAKTIO010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.denovo.458"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..>272
                     /locus_tag="O0293_002985"
     rRNA            <1..>272
                     /locus_tag="O0293_002985"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           71 a           64 c           77 g           60 t
ORIGIN      
        1 gcgactttct ggtctgtaac tgacgctgat gtgcgaaagc gtggggatca aacaggatta
       61 gataccctgg tagtccacgc cgtaaacgat gagtgctaag tgttaggggg tttccgcccc
      121 ttagtgctgc agctaacgca ttaagcactc cgcctgggga gtacgaccgc aaggttgaaa
      181 ctcaaaggaa ttgacgggga cccgcacaag cggtggagca tgtggtttaa ttcgaagcaa
      241 cgcgaagaac cttaccaaat cttgacatcc tt
//
LOCUS       DAKTIO010000425         6868 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.guided.141, whole genome shotgun sequence.
ACCESSION   DAKTIO010000425 DAKTIO010000000
VERSION     DAKTIO010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6868
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.guided.141"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            245..430
                     /locus_tag="O0293_002986"
     CDS             245..430
                     /locus_tag="O0293_002986"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281774.1"
                     /translation="MNKLEKWSLVFIIFTFLSSLALLYFKYNFSSVMPVLSLSILTIL
                     ILLTSIITVVSVNDEDT"
     gene            complement(653..1231)
                     /locus_tag="O0293_002987"
     CDS             complement(653..1231)
                     /locus_tag="O0293_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCY0281775.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNG
                     TPIKTIAEQWQVSRTTIYRYLNKLEAKENKNK"
     gene            complement(1495..1875)
                     /gene="blaI"
                     /locus_tag="O0293_002988"
     CDS             complement(1495..1875)
                     /gene="blaI"
                     /locus_tag="O0293_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HCY0281776.1"
                     /translation="MTNKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            complement(1865..3622)
                     /gene="blaR1"
                     /locus_tag="O0293_002989"
     CDS             complement(1865..3622)
                     /gene="blaR1"
                     /locus_tag="O0293_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HCY0281777.1"
                     /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTML
                     AGLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFTTDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIV
                     IRKAEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFN
                     IFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTPFSN
                     KNILSHSFNGKKSLLKSRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSL
                     TDYNYKKPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYK
                     IYLALFGLDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIP
                     KNYTAAQLKQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQ
                     LSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGK
                     NAELISEKILKGMGVLNDQ"
     gene            3729..4574
                     /gene="blaZ"
                     /locus_tag="O0293_002990"
     CDS             3729..4574
                     /gene="blaZ"
                     /locus_tag="O0293_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HCY0281778.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALN
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
     gene            complement(5100..5519)
                     /locus_tag="O0293_002991"
     CDS             complement(5100..5519)
                     /locus_tag="O0293_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0281779.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLIN
                     SNLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWP
                     TLEKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
     gene            5643..6614
                     /locus_tag="O0293_002992"
     CDS             5643..6614
                     /locus_tag="O0293_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCY0281780.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKD
                     MLALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEY
                     AQVPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGG
                     VGSVAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLD
                     TVGSNVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIR
                     EDIWSKLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
     gene            complement(6786..>6868)
                     /locus_tag="O0293_002993"
     CDS             complement(6786..>6868)
                     /locus_tag="O0293_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCY0281781.1"
                     /translation="FVHIIAPVISGCAVAYYTYWLSKRNK"
BASE COUNT         2400 a          945 c          950 g         2573 t
ORIGIN      
        1 gttaaaatta tactataaat tcaactttgc aacagaacct catttactat atgggcatct
       61 ctttcattat taatacaaag ctttgtatct ccgatattag gcaaaggtgt aaacgaatta
      121 ggtgatacta taggattcaa cattatatta atcgtttctt taataatgat tatcttctta
      181 ttttttataa atcgtagtaa aaatatctcc tatgattata taaaagttca aagaggtgaa
      241 gccaatgaat aaattagaaa aatggtcttt ggtatttatt atttttacat ttctctcttc
      301 attagcactt ttatatttca aatataattt tagttcggtt atgccagtcc taagtctttc
      361 tattttaact atactaatat tgttgacgag tattattact gtagtttcag ttaatgatga
      421 agatacttaa aacgttcatt agtgaaacag gtattatatt aaaaaagcgt acgaatttgt
      481 acgctttttt cttgtgttat tattaaaatc tcataacagg aaaatattat gagattttag
      541 acgctgtcag aacgtctctt ttacatcgag tgacattgaa tcaaagtctt tgtatgttaa
      601 tgatatataa aattatttag tagattcatt atagtaagct agattagttg ctctacttgt
      661 ttttattctc ttttgcctct aatttattga gataacgata aatagttgta cgtgaaactt
      721 gccactgctc tgcaattgtt ttaattggcg taccattatc ataaagtgtc ttaagtaagt
      781 ttaaatcttg tttatttaat ttttcaggtc tacctccata tcggcctcta gcacgtgctg
      841 caattcgacc ggctgatgat cgctctaaaa ttaaattacg ttcaaactct gcaaatgctg
      901 cgaataaatg aaataataat tgtcctgtag aacttgattt atccatcgtt atattttctt
      961 ctaaactatg aaaactcaca cctcgttcgt taagttcatt aactaacgtg attaaatcct
     1021 ccatgttccg tcctagtcta tctaatctcc aaacaacaat cgtatctcct gacctcgcaa
     1081 attcaattgc tttatctaaa ccaggtcgct tactttttga accactcata tggtcattaa
     1141 atatcttttc acaaccatat gtatttaatc gatcctcctg taaattcaaa ttttgtaatc
     1201 cagttgaaac tcttgcataa cctattttca aaatacagac ctcctatata cacctttagt
     1261 gtaccataac ttgattacga ggtcttttgt gaacatagat tttttgttgg gtttttgaac
     1321 acataattct atgtaatact tacttattta gacaaaaaac aagagtgtac agaaaatggt
     1381 tattttctgg acactctcat ctttaataat atattttaaa tgtcattaac aaaactaatt
     1441 taataagagt caagtatggt ttacatatgt attatgtttg aaacatacct ctaattactt
     1501 tttactaata tcatttaaaa tgtctcgcaa ttcttcaatt tctttgttat ttaattcttc
     1561 atttttcgca aaattcagca ctaaactttt catatcccct ccatacagtt tattaagaaa
     1621 ggttttagca gttttcattt taatatcgtc ttctttaata tttgatgagt aaaaataaat
     1681 attttctgat ttgtatcgtt ttataatctc ttttttatat agtcttgtga ttaatgttct
     1741 aatcgtttta tcactaactt ctttatattt ttgaatttca actacaattt cattagctga
     1801 tactgatttt ttatcccata ttatattcat aacatcccat tcagccatag atatttcaac
     1861 ttgcttattg gtcatttaaa acacccattc cttttaatat tttttcactt attaattcag
     1921 catttttccc agacgggttt ccatctgata aatgtgtagc aaaataatac ttatcatgat
     1981 ttgtaattac gtaacctaca aaccacccat tattatactt cccgtttact atacctgtac
     2041 ctgttttccc atacagttca tacttttcat ttttcttaat caataatgaa gaagataatt
     2101 gattctttgc ttttttacta aaatggttat tttgttccat catattttta aaaactatta
     2161 cttgttcaag attagatatt ttcaaactat cttccatcca atagctttta taacttccca
     2221 aattttcatt accataattt aattgcttga gttgagccgc agtatagttc tttggtattt
     2281 gattactaat acgttcgaag taccaattaa ctgaattttg cattgctgta tttaaatctt
     2341 gttccttatt ccaagactcg aaaagataat gtttatgatt ccatgacata cgtgaatttt
     2401 tatcacttat aatatgacgg tcaagtccaa acagtgctaa ataaattttg taagttgaat
     2461 caggagaata tctttttcta ctttcttttt cattataaat atagtatttg tctttcttca
     2521 tgctgtacat aacgaaagat ccactatttg aaccaaaatt tttactttca tctaaaattt
     2581 ggtaatcgct ttgtaagggc tttttataat tataatcagt taaggattgc cccattaaaa
     2641 actggctttg aattatcatg ataaaaaaag taaaaataca aataaaaatt agaattaatt
     2701 ttgactgctt tttcagatcg gcttctttta tattaattaa tctacttttg agtaatgatt
     2761 ttttaccatt aaatgaatgg cttaatatat ttttgttgga aaatggggtt tttaatacgg
     2821 agtccataat agattccgca taagttttaa attcattttt gttaatatta ttaaggacaa
     2881 atctatcggc ttctacttca ttgtcatgaa ttatctttct ttttacaata tgtactaatg
     2941 gattgtaact cataataata gagaagatat taaagataat taaatgtaaa gtatctctat
     3001 ttttagcatg agcatattca tgcagtatta tatattttag tcttttatca attacacttt
     3061 taaaatatga actaggaatc aaaataatat atttcccata ccaaaaagtt attggagatt
     3121 gaatagcctc tgcttttcga atcacaatat tttttttata ttgatggttg aaaagtatcg
     3181 tatctatttt atttttttca ttttcgttta gataaagtga ctgtttcttt aaatatttaa
     3241 gatataataa ggctttcaaa aatttaaaac ttaaaataat aactaaaact atccaaataa
     3301 ctgtgcagat attatcaatt gaatcccaat taaacttatg gatatctgtt gtgaactctt
     3361 gaataggttt ggtggtattt atgttatggt tcaaatcgtg tgacttactt tcaactgtgg
     3421 gcgcttgatt attcacatta ttaaatttta taaaagagaa tttaattggg ataaaaggaa
     3481 ttaatccagc aagcatagtt agataccaaa ctttataatt taaactataa ttaaaatagc
     3541 gttttaatat gtacctaaaa aatactaaca atagaaatat aaaacaaaag cttactacgc
     3601 tcattattag taatttagcc attttgacac cttctttcaa atatttataa taaacaattg
     3661 acatcaatat tacaattgta atattattga tttataaaaa ttacaactgt aatatcggag
     3721 ggtttatttt gaaaaagtta atatttttaa ttgtaattgc tttagtttta agtgcatgta
     3781 attcaaacag ttcacatgcc aaagagttaa atgatttaga aaaaaaatat aatgctcata
     3841 ttggtgttta tgctttaaat actaaaagtg gtaaggaagt aaaatttaat tcagataaga
     3901 gatttgccta tgcttcgact tcaaaagcga taaatagtgc tattttgtta gaacaagtac
     3961 cttataataa gttaaataaa aaagtacata ttaacaaaga tgatatagtt gcttattctc
     4021 ctattttaga aaaatatgta ggaaaagata tcactttaaa agaacttatt gaggcttcaa
     4081 tgacatatag tgataataca gcaaacaata aaattataaa agaaatcggt ggaatcaaaa
     4141 aagttaaaca acgtctaaaa gaactaggag ataaagtaac aaatccagtt agatatgaga
     4201 tagaattaaa ttactattca ccaaagagca aaaaagatac ttcaacgcct gctgctttcg
     4261 gcaagacttt aaataaactt atcgcaaatg gaaaattaag caaaaaaaat aaaaatttct
     4321 tacttgattt aatgtttaat aataaaaacg gagacacttt aattaaagat ggtgttccaa
     4381 aagactataa ggttgctgat aaaagtggtc aagcaataac atatgcttct agaaatgatg
     4441 ttgcttttgt ttatcctaag ggccaatctg aacctattgt tttagtcatt tttacgaata
     4501 aagacaataa aagtgataag ccaaatgata agttgataag tgaaaccgcc aagagtgtaa
     4561 tgaaggaatt ttaatattct aaatgcataa taaatactga taacatctta tagtttgtat
     4621 tatattttgt attatcgttg acatgtataa ttttgatatc aaaaactgat tttcccttta
     4681 ttattttcga gatttatttt cttaattctc tttaacaaac tagaaatatt gtatatacaa
     4741 aaaatcataa ataatagatg aatagtttaa ttataggtgt tcatcaatcg aaaaagcaac
     4801 gtatcttatt taaagtgcgt tgcttttttc tcatttataa ggttaaataa ttctcatata
     4861 tcaagcaaag tgacaatagc attttacaag tattttttat tgttcgatta gggtaaacct
     4921 aaataataca taaattatat tacaatatgt ttattataga ggctaaagta tcttagaaat
     4981 ttggatagta ggtttttcgg gaataaaagt atagttggct ttaatatata attaagctaa
     5041 gctatcattt atatttactc gattataatt cgcacttcaa cttttttata acgtaaaaat
     5101 taaaacgtag ttttatttat ctttagtaat atctttttaa acactacagt ttcttcatta
     5161 gttaaattag cgaagtaatg attattaagt tgataggctt tcttttctaa tgttggccat
     5221 aagtcttttg ctttatttgt actgtttatt agcacagttc ttctattttc attatcagtt
     5281 tgacgtgtta ttagatcctt tttggacatc ttatctaata ttccagatat tgttgaaggt
     5341 tctaaattta aagtattacc aatcgcttta ggtgtattca aattactatt aatcaagcaa
     5401 ttcaaaacac catattctgc tggagtaata ttatagggac ttaataaact tttaaaatgt
     5461 ttaaaaactc tattctgaga agtactcaac aaataattta tacaattatc taattccaca
     5521 actaaaacct ctcttaaatc attagtattg actcgcttat catatcatga taagttttgt
     5581 aggaaaatag ttttgcaaca aaaacatact tctacaaaac aattgtgaaa gaaggctaat
     5641 aaatgttcaa tgcggtagta gtgagaaaaa gtgcatcaaa tgttagttat aaagtagaaa
     5701 aagttgatga atctcattta tcgaagggtg atgtattagt aaaagtggta tactcttcaa
     5761 taaattataa ggatatgcta gctttacaat ataaaggcgg tgtaattaga gattatccta
     5821 tgattcctgg tatagatttt gcaggtattg ttgaatcaag ttcgaatgat aagtttaaag
     5881 aaggcgataa tgtattagtt actggttatg atgttggcgt gacacataca ggtggatttt
     5941 cagaatatgc acaagtgcct ggagagtgga ttgttcctat tccccaagaa atgtctatta
     6001 aagagtctat ggtatatggc acagccggtt ttacggcagc attatcaatt tttgaactag
     6061 aaaaatatgg aatgaatatc aaatcacaac ctgaaatttt agtgtctggg gctacaggtg
     6121 gtgtgggcag tgttgctata caaattttac ataaaattgg atatgaaaat atcactgcta
     6181 tcgtaagaaa agaaaagcaa atagaaatcg caaaaaaatt aggtgcaaaa caagtaataa
     6241 taattgaaga tgataataaa ccgcttaaaa aaggtatttt tgattatttc ttagatacag
     6301 tggggagtaa tgtaacatta tacggtttaa aacgtctaaa ttatcaaggt gttgctactg
     6361 tatgtggaaa tgtagcagga aattcactta acgctaatat attaccgttt attcttagag
     6421 gtattaaatt aattggcatt gattctgtat atgttgatca taatatccgt gaagatatat
     6481 ggagtaagtt agcaaatgaa tggaatatag gaaattcact tctatacaat gaaattggat
     6541 ttgaagaatt ctataaaacg atagatcagc ttttaaaagg acaacattta ggaagaacta
     6601 ttttaaaagt ataagtgtga gtattcatta atacatttac tattaattca atcattttgc
     6661 atttctctac taaccagtgt tataatttat ataacgaaaa tgattaacaa ttaatgcacc
     6721 aatccccaca ctattgcagt agtgtgggga ttttttcgat gctggcttat aaagtcgcct
     6781 attaattatt tgttgcgttt actaagccaa taagtataat acgcaacagc acagccactg
     6841 atgactggtg ctatgatgtg aacgaaaa
//
LOCUS       DAKTIO010000426         6589 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.guided.145, whole genome shotgun sequence.
ACCESSION   DAKTIO010000426 DAKTIO010000000
VERSION     DAKTIO010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6589
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.guided.145"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            414..1880
                     /gene="msr(A)"
                     /locus_tag="O0293_002994"
     CDS             414..1880
                     /gene="msr(A)"
                     /locus_tag="O0293_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(A)"
                     /protein_id="HCY0281782.1"
                     /translation="MEQYTIKFNQINHKLTDLRSLNIGHLYAYQFEKIALIGGNGTGK
                     TTLLNMIAQKTKPESGTVETVGEIQYFEQLNMDVENDFNTLDGSLMSELHIPMHTTDS
                     MSGGEKAKYKLANVISNYSPILLLDEPTNHLDKIGKDYLKNILKYYYGTLIIVSHDRA
                     LIDQIADTIWDIQEDGTIRVFKGNYTQYQNQYEQEQLEQQRQYEQYISEKQRLSQASK
                     AKRNQAQQMAQASSKQKNKSIAPDRLSASKQKGTVEKAAQKQAKHIEKRMEHLEEVEK
                     PQSYHEFNFPQNKIYDIHNNYPIIAQNLTLVKGSQKLLTQVRFQIPYGKNIALVGANG
                     VGKTTLLEAIYHQIEGIDCSPKVQMAYYRQLAYEDMRDVSLLQYLMDETDSSESFSRA
                     ILNNLGLNEALDRSCNVLSGGERTKLSLAVLFSTKANMLILDEPTNFLDIKTLEALEM
                     FMNKYPGIILFTSHDTRFVKHVSDKKWELTGQSLHDIT"
     gene            1979..2878
                     /locus_tag="O0293_002995"
     CDS             1979..2878
                     /locus_tag="O0293_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(C) family macrolide 2'-phosphotransferase"
                     /protein_id="HCY0281783.1"
                     /translation="MTRHNEIIKCAEKYQLHIQPQTISLNESGLDFQVAFGKDKHGVE
                     WVLRLPRRPDVYKRTKPEKQTVDFLQKNVSFEIPKWKVHAKDLIAYPKLTGKPAATID
                     PEIQNYVWEIEHKPLPENFINTLAETLVDLHNIPEENINVQHINIKTIQEIKNDFQRR
                     MNKVKETYGVSDELWNRWKQWLENDELWPRHATMIHGYLHPGHIMVDNQANVTGLIDW
                     TEATHSDPSMDFIGHHRVFDDEGLEQLITAYGKAGGEIWPRMKEHIIELNAVFPMFIA
                     EFAMESGESAYETMALKELGMKE"
     gene            3364..3972
                     /locus_tag="O0293_002996"
     CDS             3364..3972
                     /locus_tag="O0293_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCY0281784.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRP
                     VFQEALNFVRMGDRFVVESIDRLGRNYDEIIETVNYLKEKDVQLMITSLPMMNEVIGN
                     PLLDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQ
                     KRVIYHRVVEMLEEGKAISKIAKEVNITRQTVYRIKHDKGLS"
     gene            4218..4556
                     /locus_tag="O0293_002997"
     CDS             4218..4556
                     /locus_tag="O0293_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281785.1"
                     /translation="MNLIPFIIILIFSCLWTAITNKYLPSETLDKKGQQDRYDERQRK
                     MFIEILAKSFIWIVYCMLFTLVLKFTGLSPSIEGSWFSQYPEIFFIIVALFLMLFNYY
                     TTNKKYTSKG"
     gene            4561..4746
                     /locus_tag="O0293_002998"
     CDS             4561..4746
                     /locus_tag="O0293_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0281786.1"
                     /translation="MKNRIREYRKQKGISQETLSKELKVSRQTINAIENNKYDPTLTL
                     AFKLARLFDTTVDELFS"
     gene            complement(4973..5716)
                     /locus_tag="O0293_002999"
     CDS             complement(4973..5716)
                     /locus_tag="O0293_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCY0281787.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDI
                     EKIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEK
                     QESRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTST
                     NTRQNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPA
                     NLNDNDSDIEEKSEETEPKKGFWSRLFGS"
     gene            6142..>6589
                     /locus_tag="O0293_003000"
     CDS             6142..>6589
                     /locus_tag="O0293_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCY0281788.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILR
                     DRLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGL
                     NKPNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENR"
BASE COUNT         2505 a          940 c         1018 g         2126 t
ORIGIN      
        1 tttatgagta cactctaaat taaggagatg tataatcaat ctaaaattgt atttttatac
       61 ttttgttata taatctttct acttagagat atattgtaaa aaaaccaaca aaatgttata
      121 ttaacattac tattttatcg atgaaggagg attaaaatga cagcttctat gagactcaaa
      181 taaacctttt tgaatacaaa actaaattaa ttcaaaaagg cttgtccgca atacacactc
      241 ataggatgac ttatgtcatt ttttatcctt cgataggaat aataaacgct ttcaaatact
      301 aaaacatcat aataaatttt taagtgtttg aaagtgtaat gtgaatgaga cgggagtagc
      361 ctgtctcatt tttttgcgta cacgtccttt aaatatacaa ggagtgtata aatatggaac
      421 aatatacaat taaatttaac caaatcaatc ataaattgac agatttacga tcacttaaca
      481 tcggtcatct ttacgcttac caatttgaaa aaatagcact tattgggggt aatggtactg
      541 gcaaaaccac attactaaat atgattgctc aaaaaacaaa accagaatct ggaacagttg
      601 aaacggttgg cgaaattcaa tattttgaac agcttaacat ggatgtggaa aatgatttta
      661 acacgttaga cggtagttta atgagtgaac tccatatacc tatgcataca accgacagta
      721 tgagtggtgg tgaaaaagca aaatataaat tagctaatgt catatcaaat tatagtccga
      781 tattactttt agatgaacct acaaatcact tggataaaat tggtaaagat tatctgaaaa
      841 atattttaaa atattactat ggtactttaa ttatagtaag tcatgataga gcacttatag
      901 accaaattgc tgacacaatt tgggatatac aagaagatgg cacaataaga gtgtttaaag
      961 gtaattacac acagtatcaa aatcaatatg aacaagaaca gttagaacaa caacgtcaat
     1021 atgaacagta tataagtgaa aaacaaagat tgtcccaagc cagtaaagct aaacgaaatc
     1081 aagcgcaaca aatggcacaa gcatcatcaa aacaaaaaaa caaaagtata gcaccagatc
     1141 gtttaagtgc atcaaaacaa aaaggcacgg ttgagaaggc tgctcaaaaa caagctaagc
     1201 atattgaaaa aagaatggaa catttggaag aagttgaaaa accacaaagt tatcatgaat
     1261 tcaatttccc acaaaataaa atttatgata tccataataa ttatccaatc attgcacaaa
     1321 atctaacatt ggttaaagga agtcaaaaac tgctaacaca agtacgattc caaataccat
     1381 atggcaaaaa tatagcgctc gtaggtgcaa atggtgtagg taagacaact ttacttgaag
     1441 ctatttacca ccaaatagag ggaattgatt gttctcctaa agtgcaaatg gcatactatc
     1501 gtcaacttgc ttatgaagac atgcgtgacg tttcattatt gcaatattta atggatgaaa
     1561 cggattcatc agaatcattc agtagagcta ttttaaataa cttgggttta aatgaagcac
     1621 ttgatcgttc ttgtaatgtt ttgagtggtg gggaaagaac gaaattatcg ttagcagtat
     1681 tattttcaac gaaagcgaat atgttaattt tggatgaacc aactaatttt ttagatatta
     1741 aaacattaga agcattagaa atgtttatga ataaatatcc tggaatcatt ttgtttacat
     1801 cacatgatac aaggtttgtt aaacatgtat cagataaaaa atgggaatta acaggacaat
     1861 ctcttcatga tataacttaa aaactataca ctaatttata ttgaatatta ggttcattta
     1921 tatcaatata aaaaatacgt tcacaagaaa aatataatag aaataaggat ccaataaaat
     1981 gactcgacat aatgaaatta ttaaatgtgc agaaaaatat caattacaca tccaacctca
     2041 aacaatctca ttgaatgaat cgggacttga ttttcaagtt gcatttggaa aagataaaca
     2101 tggagtagaa tgggttttga gactaccaag aagacctgac gtttataaac gaacaaaacc
     2161 cgaaaaacaa acggtagact tcttacagaa gaatgtttca tttgaaatac cgaagtggaa
     2221 agtacatgca aaagacctta ttgcttaccc aaaacttaca ggtaaacccg cagccacaat
     2281 agatccagaa atacaaaatt atgtatggga aattgaacac aaaccattac cagaaaactt
     2341 tattaacaca ttagctgaaa cactcgtaga tttacacaac ataccagaag aaaacattaa
     2401 cgttcagcat ataaatatca aaaccataca agaaataaaa aatgactttc aaagaagaat
     2461 gaataaagtt aaagaaactt atggcgtatc agatgaatta tggaacagat ggaaacaatg
     2521 gttagaaaac gacgaactat ggcctcgaca tgcgaccatg atacatgggt acttacatcc
     2581 aggacatata atggtagata accaagcaaa cgtcacaggt ctcatagact ggactgaagc
     2641 aacccactcc gacccatcaa tggactttat aggacaccat cgtgtattcg acgacgaagg
     2701 attagagcaa cttataacag catacggtaa agctggaggt gaaatatggc cacgaatgaa
     2761 agagcatata atagaactca atgcagtatt cccaatgttt atcgctgagt ttgctatgga
     2821 atcaggagaa tcggcgtatg aaacgatggc attgaaagag ttaggtatga aagagtagaa
     2881 cttatattaa aagaggccca ttttaaagac gaaggcaatc ctgatgaaat tttaaaactg
     2941 cgtttaatta gtgatggtct ttggttttct ataatgtatt aaatatctgg acatggaaca
     3001 aatgcaacaa atggaaagaa tcgttttcga aatttctaac tccttaaatc aggaggataa
     3061 ataaacggtc attttcattg aaagcactta tataaatttc atcaatttta atacctaccg
     3121 taaatacatt tcataagtat taaacagatg attaaaattt ttagttaatt taaatattta
     3181 tgatagagat gaccttcaaa tgaacggttc tgttacaaag aaaaaatcta tatttatttt
     3241 tagtaagttt taattataga tatgtacact ttgagtacac cctaaccata caaaaagaat
     3301 tgttgaaatt ataacaatta aaacgtatga ttagggtgta tatttattat ggagtgaaga
     3361 tgaatgatta taggttatgc gagagtatca tcgatagatc aaaatttaga aagacaattg
     3421 gataatttaa agacgtttgg tgtggagaaa atctttacag agaaacagtc gggaaagtca
     3481 gtggaaaata gacctgtatt tcaagaagca ctcaattttg tcagaatggg agatcgattc
     3541 gttgtggaat ctattgatcg tttaggacgt aactatgatg aaattattga aactgtgaat
     3601 tatttaaaag aaaaagacgt tcagttaatg attaccagct tacctatgat gaatgaagta
     3661 attggcaatc cattactgga taagtttatg aaagatttaa tcattcaaat attagcaatg
     3721 gtttcagaac aagagagaaa tgaaagtaaa cgtcgacaag cgcaagggat tcaagttgcg
     3781 aaggaaaaag gcgtatataa agggcgccct ttactttatt caccaaatgc taaagaccca
     3841 cagaaacgtg ttatttatca tcgcgtggtg gaaatgttag aagaaggcaa agcgattagt
     3901 aagattgcga aagaagttaa tatcacaaga caaacagttt atagaattaa acatgataag
     3961 ggattatctt gataaattga ttgtcaatgc aaatgagcgt ctctcacagt gtctcaaatt
     4021 ccataacata ttattgaatt gagattttaa taattataca agaaaaaaga gtacgagttc
     4081 gcagctttct atatagtaat tgttttacta ataaggtgtt ttaaaatata ttataatgta
     4141 aaaagtattt gacatttgta tcaaaaatat ttacactcat aaatgtcaaa tactttttac
     4201 atttatgagg tgttataatg aatttaattc cttttattat tattttaatt ttttcttgtt
     4261 tatggacggc tataactaac aaatatttac cttcagaaac attagataaa aaaggacaac
     4321 aagatcgata cgatgaaaga caacgtaaaa tgtttattga aatactggct aaaagtttta
     4381 tttggattgt atattgtatg ttatttacac tagtgcttaa atttacaggt ttgtcaccaa
     4441 gtattgaagg atcttggttt tctcaatatc cagaaatttt ctttataatt gtagcactat
     4501 ttcttatgct gtttaattac tatactacaa ataaaaaata tacatctaaa ggttaaatta
     4561 atgaaaaata gaattagaga atatagaaaa caaaaaggaa tttctcaaga aactttaagt
     4621 aaagaattaa aagtgtctag gcaaactatt aatgcaatag aaaacaataa atatgatcct
     4681 acattgacat tagcatttaa gttggcaaga ttgtttgata caactgtgga tgaattattt
     4741 tcttaaccat tcatttatca caaaaagaga gtgcgaattc gcactctctt tttgtgtagt
     4801 agtactatta aattttgtct caataacgaa cgtttataac gcaatcatga tgagagtatt
     4861 aatacagtta attatttgaa atactttagt ttacataatc gatgattacc agaagttctc
     4921 actatataaa agacactaaa attttataac ttttagtgcc taatcaatta ttttagctac
     4981 caaacaaacg actccaaaac ccctttttag gttccgtttc ttcactcttt tcttctatat
     5041 cactatcatt gtcatttaga ttagctggaa cgtcttcttt ttttgcttct tcaccttgtg
     5101 tactttcgtc gctttgactt ccagatatat ccttatattg aatctctgtt tctttagttt
     5161 cttcctgatc tctgttaata tttacaggat tagtagtata tgttttttct tgtgcatcaa
     5221 cgttttgtct agtgttagta gatgtatcaa agggataatt aaggtgtttt tctttttcta
     5281 attgattttc taatttttga atctttttat tactctctaa tgccagaact tgctgatttt
     5341 caagcaattt tgatctttct tgcaactctt ttctgtaaaa ttcaattgtt gtttcatgac
     5401 gactttcttg tttttcaatt tgattattta aagtatcaat ctgattttta aggatttcta
     5461 ctatttgagc atcgcttttc gattcaaaat taatattatc cgaccctttt ttttgtttgt
     5521 cttcagtagt actttcaaaa ccatatttct ttttattttc atttactctt tgaatgattt
     5581 tttctatatc ggtatcttct ttaataaacg aagtgttttc ctgttttatt gtctctatat
     5641 tcaatcgctt aatattatta aaaatcgttt gcttactaac ccctaattct tcactcaact
     5701 ttttcacact tttcatatca tcaacccttt ataaaatgtt tataaaccct tgataaaata
     5761 ttataagctt ataatgctta ataataaagt gattaataaa taaaaataaa catccatcaa
     5821 cctttttatc aagggtttat gaaaggttta tgatatttat caagggttta taaacccttg
     5881 ataaagctat attttccctt ttataagttt atttaaagtg ctataataaa cttaatataa
     5941 caaaaacccc aactactggt aatagtcggg gtttggtaat aaaacgttta gaaactgttt
     6001 atttatataa ttttcgatac ttgtattata taatgaataa tatcttagtg caagcaccta
     6061 aatcattaaa taaatgtaaa gctaaataaa aacgttttat tacctcttac caaactaaaa
     6121 tatggaaaga ggttttttgt catgcaaaat caatatttta cagtgcaaga aaattacaaa
     6181 gaaagatttt atcaaatccc taaagtattc tttaccagtg aaaattataa aaatctaact
     6241 aatgatatga aaatagccta tgctatctta cgtgatcgct taaatttatc tattaaaaat
     6301 agctgggttg atgaagatgg taacatttac tttgtttatt caaatgaaaa gctaatggaa
     6361 atcttaaatt gtaaaaaaga aaagttaaca aaaataaaaa aagggttaga aaacgatggt
     6421 ctactcattc aaaaacgacg tggtttaaat aaaccaaata ttctatattt aatgaaacct
     6481 attgtcacag aacgtgatat ttacaaaata gaaaaagaag aaaacgatgt tgaaccttac
     6541 ggcgagaagg aagttcgaaa atcgaacgtc cagaagttcg aaaatcgaa
//
LOCUS       DAKTIO010000427         4935 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.guided.181, whole genome shotgun sequence.
ACCESSION   DAKTIO010000427 DAKTIO010000000
VERSION     DAKTIO010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.guided.181"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..254
                     /locus_tag="O0293_003001"
     CDS             <1..254
                     /locus_tag="O0293_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HCY0281789.1"
                     /translation="VFGFMKAILGFTRMSVRGLNKVKRELGFVLMALNIRKVVAQRAE
                     NNQKIYKKDNFYIISIEIVFFHLSKNFMSRTHYDLFFYC"
     gene            complement(245..505)
                     /locus_tag="O0293_003002"
     CDS             complement(245..505)
                     /locus_tag="O0293_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HCY0281790.1"
                     /translation="MYVVYRASHETAKEALGDKELRAIAHELTKTVKDNMSVDWSKRD
                     SAKAKMRVQVRRLLKKYGYPPDLQKMAVEQVVEQAELMASQQ"
     gene            complement(477..1463)
                     /locus_tag="O0293_003003"
     CDS             complement(477..1463)
                     /locus_tag="O0293_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer MecR1"
                     /protein_id="HCY0281791.1"
                     /translation="MLSSFLMLSIISSLLTICVIFLVRMLYIKYTQNIMSHKIWLLVL
                     VSTLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVTKDLAINVNQFET
                     SNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERLKVCQSKMQFYKK
                     HITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHELSHVKSHDLIFNQ
                     LYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRYGESILKCSILKS
                     QHINNVAAQYLLGFNSNIKERVKYIALYDSMPKPNRNKRIVAYIVCSISSFT"
     gene            1560..3569
                     /gene="mecA"
                     /locus_tag="O0293_003004"
     CDS             1560..3569
                     /gene="mecA"
                     /locus_tag="O0293_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase MecA"
                     /protein_id="HCY0281792.1"
                     /translation="MMKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFK
                     QVYKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKI
                     KTNYGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNN
                     VELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTV
                     KKMDEYLSDFAKKFHLTTNETESRNYPLGKATSHLLGYVGPINSEELKQKEYKGYKDD
                     AVIGKKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQ
                     KSIYNNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPL
                     LNKFQITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV
                     NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKN
                     LDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISK
                     ENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKD
                     NPNMMMAINVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
     gene            complement(3615..4043)
                     /locus_tag="O0293_003005"
     CDS             complement(3615..4043)
                     /locus_tag="O0293_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HCY0281793.1"
                     /translation="MKYDDFIVGETFKTKSLHITEEEIIQFATTFDPQYMHIDKEKAE
                     QSRFKGIIASGMHTLSISFKLWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAE
                     ITNKKSIKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
     gene            complement(4140..4883)
                     /locus_tag="O0293_003006"
     CDS             complement(4140..4883)
                     /locus_tag="O0293_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HCY0281794.1"
                     /translation="MLTVYGHRGLPSKAPENTIASFKAASEVEGINWLELDVAITKDE
                     QLIIIHDDYLERTTNMSGEITELNYDEIKDASAGSWFGEKFKDEHLPTFDDVVKIANE
                     YNMNLNVELKGITGPNGLALSKSMVKQVEEQLTNLNQNQEVLISSFNVVLVKLAEEIM
                     PQYNRAVIFHTTSFREDWRTLLDYCNAKIVNTEDAKLTKAKVKMVKEAGYELNVWTVN
                     KPARANQLANWGVDGIFTDNADKMVHLSQ"
BASE COUNT         1763 a          780 c          740 g         1652 t
ORIGIN      
        1 ctgtttttgg atttatgaag gctattttgg gtttcactcg gatgtctgtc cgaggactca
       61 ataaagtcaa aagagaactt ggttttgtat taatggcact taatataaga aaagtagtag
      121 ctcaacgagc tgaaaataat caaaaaattt ataaaaaaga caatttctat attatttcaa
      181 tagaaattgt cttttttcac ttatccaaga actttatgtc ccggactcat tatgatttat
      241 ttttttattg ctgacttgcc attaattctg cttgctctac aacttgttcc acagccattt
      301 tttgaagatc tggtggatag ccatatttct ttaataggcg tctaacttga actctcattt
      361 tagctttagc actgtctcgt ttagaccaat caacactcat gttatcctta actgttttag
      421 ttaactcatg tgcaatggct cttaactctt tatcgcccaa agcttcttta gctgtttcat
      481 gtgaagctcg atatactaca tacaatatac gcaacaatac gcttgtttcg attaggttta
      541 ggcattgaat cataaagtgc aatatactta acacgttctt taatatttga attaaaacct
      601 agtaaatatt gtgctgccac attatttatg tgctgagatt ttaaaataga gcattttaat
      661 atcgattcac cataacgtat atgttcatgg cgattcaaaa tttttaaaac gtttctatca
      721 catacttttt cacagtcatt gtccatcatt gttttactta tatatagtgc aggattaaac
      781 cagaatatca ttttaaaaac aacataaagc tggttgaata ttaagtcatg acttttcaca
      841 tgtgatagtt catgtagaat aatatattca atttctttgt cattcatggt ttcgactacg
      901 acagttggta gtacaatttg ggatttcact aaaccaaata ccatcggatt atcaatgttt
      961 gaactataac taattgttat atgctttttg tagaactgca tcttactttg acatacttta
     1021 agtcgttcat taagatatga cgattccaat gacgaacttt taataacatc aatttgtcgg
     1081 aatgccttaa tcatataaaa taagcacaac aaactaccaa atacccatat caaaagaatc
     1141 atatacgtta tatttgaggt ctcaaactga ttaacattaa ttgctaagtc tttcgtaaca
     1201 gatgattgtt gaccatctaa catatgacta accgaagaag tcgtgtcaga tacatttcga
     1261 ttcatcatat cttttgaaaa tgtaaaattc gatattttgt aaaatggtat taatggaatt
     1321 aacgtggaga cgagcactaa taaccaaatc ttatgtgaca taatattttg agtatatttt
     1381 atatagagca ttctcactaa aaaaattaca catatcgtga gcaatgaact gattatactt
     1441 aacattaaaa aagatgataa caccttctac acctccatat cacaaaaatt ataacattat
     1501 tttgacataa atactacatt tgtaatatac tacaaatgta gtcttatata aggagtatat
     1561 tgatgaaaaa gataaaaatt gttccactta ttttaatagt tgtagttgtc gggtttggta
     1621 tatattttta tgcttcaaaa gataaagaaa ttaataatac tattgatgca attgaagata
     1681 aaaatttcaa acaagtttat aaagatagca gttatatttc taaaagcgat aatggtgaag
     1741 tagaaatgac tgaacgtccg ataaaaatat ataatagttt aggcgttaaa gatataaaca
     1801 ttcaggatcg taaaataaaa aaagtatcta aaaataaaaa acgagtagat gctcaatata
     1861 aaattaaaac aaactacggt aacattgatc gcaacgttca atttaatttt gttaaagaag
     1921 atggtatgtg gaagttagat tgggatcata gcgtcattat tccaggaatg cagaaagacc
     1981 aaagcataca tattgaaaat ttaaaatcag aacgtggtaa aattttagac cgaaacaatg
     2041 tggaattggc caatacagga acagcatatg agataggcat cgttccaaag aatgtatcta
     2101 aaaaagatta taaagcaatc gctaaagaac taagtatttc tgaagactat atcaaacaac
     2161 aaatggatca aaattgggta caagatgata ccttcgttcc acttaaaacc gttaaaaaaa
     2221 tggatgaata tttaagtgat ttcgcaaaaa aatttcatct tacaactaat gaaacagaaa
     2281 gtcgtaacta tcctctagga aaagcgactt cacatctatt aggttatgtt ggtcccatta
     2341 actctgaaga attaaaacaa aaagaatata aaggctataa agatgatgca gttattggta
     2401 aaaagggact cgaaaaactt tacgataaaa agctccaaca tgaagatggc tatcgtgtca
     2461 caatcgttga cgataatagc aatacaatcg cacatacatt aatagagaaa aagaaaaaag
     2521 atggcaaaga tattcaacta actattgatg ctaaagttca aaagagtatt tataacaaca
     2581 tgaaaaatga ttatggctca ggtactgcta tccaccctca aacaggtgaa ttattagcac
     2641 ttgtaagcac accttcatat gacgtctatc catttatgta tggcatgagt aacgaagaat
     2701 ataataaatt aaccgaagat aaaaaagaac ctctgctcaa caagttccag attacaactt
     2761 caccaggttc aactcaaaaa atattaacag caatgattgg gttaaataac aaaacattag
     2821 acgataaaac aagttataaa atcgatggta aaggttggca aaaagataaa tcttggggtg
     2881 gttacaacgt tacaagatat gaagtggtaa atggtaatat cgacttaaaa caagcaatag
     2941 aatcatcaga taacattttc tttgctagag tagcactcga attaggcagt aagaaatttg
     3001 aaaaaggcat gaaaaaacta ggtgttggtg aagatatacc aagtgattat ccattttata
     3061 atgctcaaat ttcaaacaaa aatttagata atgaaatatt attagctgat tcaggttacg
     3121 gacaaggtga aatactgatt aacccagtac agatcctttc aatctatagc gcattagaaa
     3181 ataatggcaa tattaacgca cctcacttat taaaagacac gaaaaacaaa gtttggaaga
     3241 aaaatattat ttccaaagaa aatatcaatc tattaactga tggtatgcaa caagtcgtaa
     3301 ataaaacaca taaagaagat atttatagat cttatgcaaa cttaattggc aaatccggta
     3361 ctgcagaact caaaatgaaa caaggagaaa ctggcagaca aattgggtgg tttatatcat
     3421 atgataaaga taatccaaac atgatgatgg ctattaatgt taaagatgta caagataaag
     3481 gaatggctag ctacaatgcc aaaatctcag gtaaagtgta tgatgagcta tatgagaacg
     3541 gtaataaaaa atacgatata gatgaataac aaaacagtga agcaatccgt aacgatggtt
     3601 gcttcactgt tttattatga attattaata agtgctgtta cttctccctt aaatacaatt
     3661 tcttcatttt cattgtatgt tgaaagtgac actgtaacga gtccattttc tttttttatg
     3721 gatttcttat ttgtaatttc agcgataacg tacaatgtat tacctgggta tacaggttta
     3781 ataaatttaa cgttattcat ttgtgttcct gctacaactt cttctccgta tttaccttct
     3841 tctacccata atttaaatga tattgaaagt gtatgcatgc cagatgcaat gataccttta
     3901 aatctacttt gttctgcttt ttctttatct atatgcatat attgaggatc aaaagttgtt
     3961 gcaaattgga taatttcttc ttctgtaata tgaaggcttt ttgttttgaa tgtttctcct
     4021 actataaaat catcgtattt catatatgtc tctctttctt attcaaatta attttttagt
     4081 atgtaacatg ttaaaggtaa gtctaccgtc actgaaacgt aagactcacc tctaactttc
     4141 tattgagaca aatgcaccat tttatctgca ttgtctgtaa agataccatc aactccccaa
     4201 ttagcaagtt ggtttgcacg tgctggtttg tttacagtcc atacgttcaa ttcataaccc
     4261 gcttctttta ccatttttac ttttgcttta gtaagtttgg catcttcagt gtttactatt
     4321 ttagcattac agtaatctaa aagtgttctc cagtcttcac gaaacgaagt tgtatggaat
     4381 ataactgctc tgttatattg tggcatgatt tcttctgcaa gtttaacaag cacaacatta
     4441 aagcttgaaa tgagcacttc ttgattctga tttaagtttg ttaattgttc ttccacttgc
     4501 ttaaccatac ttttagaaag tgctagtcca ttcggtccag taataccttt taattctaca
     4561 tttaaattca tattatattc atttgctatt tttactacat catcgaaagt tggcaaatgt
     4621 tcatctttga atttttcacc aaaccaagat cctgcagaag catctttaat ttcatcataa
     4681 ttcaattcag ttatttcccc ggacatattt gtagtccgtt ctaaataatc atcatgaatg
     4741 ataatcagtt gttcatcttt tgtaattgca acatctaact ccaaccagtt tataccttct
     4801 acttctgaag cagctttaaa tgatgcaatt gtattttccg gagctttact aggtaatcct
     4861 ctatgtccat atacagttag catattacct ctccttgcat ttttattttt ttaattaacg
     4921 taactgtatt atcac
//
LOCUS       DAKTIO010000428         4919 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.guided.183, whole genome shotgun sequence.
ACCESSION   DAKTIO010000428 DAKTIO010000000
VERSION     DAKTIO010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.guided.183"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(286..1524)
                     /locus_tag="O0293_003007"
     CDS             complement(286..1524)
                     /locus_tag="O0293_003007"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HCY0281795.1"
                     /translation="MNDLIINHIAELILPRSTDKPLKGKELDELNVVKNGTVVIKDGK
                     IVYAGTHTDDYDATETIDASGKVVSPALVDAHTHLTFGGSREHEMSLKRQGKSYLEIL
                     EMGGGILSTVNATRETSEDDLFKKAEHDLLTMIKHGVLAVESKSGYGLDRENELKQLK
                     VSNRLAEKYDLDMKHTFLGPHAVPKEASSNEAFLEEMIALLPEVKQYADFADIFCETG
                     VFTIEQSQHYMQKAKEAGFKVKIHADEIDPLGGLELAIDEQAISADHLVASSDKGKEK
                     LRNSDTVAVLLPATTFYLGKEDYADARGMLDNNGAIALATDYNPGSSVTNNLQLVMAI
                     AALKLKLSPNEVWNAVTVNAAKAIDINAGTINTGDKANLVIWDAPNHEYIPYHFGINH
                     AEKVIKDGKVIVDNTVSFKA"
     gene            complement(1524..3185)
                     /gene="hutU"
                     /locus_tag="O0293_003008"
     CDS             complement(1524..3185)
                     /gene="hutU"
                     /locus_tag="O0293_003008"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HCY0281796.1"
                     /translation="MRKIQAKKGLSIECKGWEQEAVLRMLYNNLDPEVAERPEDLVVY
                     GGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEAPRVLISNSVLVP
                     EWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAELGNQHFNGDLAGT
                     VTLTAGLGGMGGAQPLAITMNHGVAICVDVDETRVDKRIDTKYCDVKTADLDEALKLA
                     EEAKERGEGLSIGLVGNAVDIHQAILEKGFKIDIITDQTSAHDPLNGYVPQGYSVEEA
                     KVLREKDPKKYVELSQASMAKHVELMLEFQKRGAVAFDYGNNIRQVAFNNGVKNAFDF
                     PGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKLFPENEKLLRWLDLAEEK
                     ISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLDAGSVASPNRETE
                     SMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGMVVVADGSERAER
                     RLERVLTTDPGMGVARHVDAGYDIAIQTAKEKGIHIPMIDKAGDK"
     gene            3345..4229
                     /locus_tag="O0293_003009"
     CDS             3345..4229
                     /locus_tag="O0293_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0281797.1"
                     /translation="MKIIQLEYFLAIVKYNSFTKAAQFLHISQPSLTATIKKMEADLG
                     YDLFTRSTKDIKITEKGIQFYRYASELVQQYRSTMEKMYDLSVTSEPRIKIGTLESTN
                     QWIANLIRKHHSDYPEQQYRLYEIHDKHQSIEQLLNFNIHLAITNEKITHEDIRSIPL
                     YEESYILLAPKETFKNQNWVDVENLPLILPNKNSQVRKHLDDYFNRRNIRPNVVVETD
                     RFESAVGFVHLGLGYAIIPRFYYQSFHTSNLEYKKIRPNLGRKIYINYHKKRKHSEQV
                     HTFVQQCQDYLYGLLEAL"
     gene            4351..4770
                     /locus_tag="O0293_003010"
     CDS             4351..4770
                     /locus_tag="O0293_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB1/FosB3 family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HCY0281798.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
BASE COUNT         1573 a         1004 c          703 g         1639 t
ORIGIN      
        1 taatcccatc ggtcattccc cttccttaag tcaatgatat gttgtctacc ctacgatgat
       61 catctttgac tattaaacga tgatttcaca acaatgtact cttgttaatt gctttcgtta
      121 atgatagaca gttgtttaat aatatcgtaa cactgttgtc aaactattct aacttttata
      181 attgagactc tatacaaaaa cgtgttctcg aatatacttg tttttacaaa ccacaaaaag
      241 ctctaaacat tagtttaaac caatgcttag agctttctaa ttattttatg ctttaaaaga
      301 tactgtgtta tctacgatga ccttaccgtc tttaataact ttttctgcgt gattgatacc
      361 aaaatgatat ggaatatatt catgatttgg tgcatcccaa attactaaat tagccttatc
      421 acctgtgtta attgtacccg cgttaatgtc tattgcttta gcagcattga ccgtaacagc
      481 attccaaact tcattaggtg atagctttaa tttcaaggct gcaatcgcca taacaagttg
      541 taagttgttt gtgacactac taccagggtt ataatcagtt gctaatgcaa tcgcaccgtt
      601 attgtcaagc atgcctcttg catctgcata atcttcttta cctaaataga acgtcgttgc
      661 aggtaagagg acagctacag tatcactatt tcgcaacttt tcttttcctt tatcactaga
      721 agctactaag tggtctgctg atattgcttg ttcatcaatt gctaattcca gtccgcctaa
      781 cggatcaatt tcatccgcat gtattttcac tttaaaacct gcttctttgg ctttttgcat
      841 ataatgttgc gattgttcta ttgtaaatac acctgtttca cagaaaatat ccgcaaagtc
      901 tgcatattgt tttacttccg gaagtaacgc aatcatttct tctaaaaatg cctcatttga
      961 acttgcctct ttaggtacag catgaggccc taggaaagta tgtttcatgt ctaaatcata
     1021 tttctcagct aaacgattag acactttcaa ttgcttcagt tcattttctc tatctaatcc
     1081 ataaccactc ttactttcaa ctgcaagcac gccgtgttta atcatagtaa gcaaatcatg
     1141 ctctgctttt ttaaacaagt catcttcgga tgtttctcta gtagcattaa cggtagataa
     1201 tatgccacca cccatttcta atatttcaag gtaagactta ccttgacgtt ttaatgacat
     1261 ctcatgttct cgagatccac caaatgttaa atgggtatgt gcatctacta atgctgggga
     1321 cactaccttc ccactagcat caatcgtctc agtcgcatcg tagtcatctg tatgtgttcc
     1381 agcatataca attttgccat ctttaatgac aactgtacca tttttcacaa catttaattc
     1441 atctaattcc ttacccttca aaggtttatc tgttgatctc ggtaaaatta attctgctat
     1501 atgattaatt attaaatcat tcattactta tcacctgctt tatcaatcat tggaatatga
     1561 ataccttttt ctttagctgt ttgaatagcg atgtcatagc cagcatcaac atgtcgggca
     1621 acacccatac ctgggtcagt cgtcaataca cgttccaatc ttctttcagc acgctctgat
     1681 ccatctgcta caacaaccat acccgcatga agtgaatatc ccatgccaac accgccaccg
     1741 tgatggaatg aaatccatga accacctgca gctgtgttaa tgagtgcatt caatacagcc
     1801 caatcaccaa ccgcgtcact accatctttc atactttctg tttcacggtt aggactagca
     1861 actgaaccag catctaaatg gtctcgtcca ataacaattg gtgctgaaat ttcaccgtca
     1921 cgtacaagac gatttaaagc taagcccatt ttcgctcttt ctccatagcc taaccaagca
     1981 atacgtgatg gtagtccttg atatgaaatt ttttcttcag ctaaatcaag ccatcttaat
     2041 aacttttcat tttctgggaa aagtttgcgc atttcttcat ccgcacgctc gatatctttt
     2101 ggatcaccac tcaacgcagc aaagcggaat ggccctttac cttcacagaa taatggtcta
     2161 atgtaagctg gtacaaagcc tgggaagtca aaagcatttt tcactccgtt attgaaggct
     2221 acttgacgaa tattgttacc ataatcaaat gctacagcgc cacgtttttg gaattcaagc
     2281 attaattcaa catgctttgc cattgaagct tgtgacagtt caacatattt tttcggatct
     2341 ttttcacgca atactttcgc ttcttctaca gagtatcctt gtggcacata tccatttagc
     2401 ggatcatgtg cacttgtttg gtcagtaata atgtcaattt taaatccttt ttctagaatc
     2461 gcttgatgga tgtctacagc atttccaact aacccgattg ataatccttc tccacgttct
     2521 ttcgcctctt ctgctaattt taatgcttca tctaaatcag ctgttttaac atcacagtat
     2581 ttcgtatcaa ttcgcttatc aacacgtgtt tcatcaacat ccacgcaaat tgctacccca
     2641 tgattcatag taattgctaa cggttgcgca ccacccatac cacctaaacc tgctgtcagt
     2701 gtaacagtgc ctgctaaatc tccattaaag tgttgattac ctagctcggc aaatgtctca
     2761 taagtacctt gcacaatacc ttgagaacca atatatatcc aactaccggc tgtcatctgt
     2821 ccatacatga ttaaaccttt tttatctaat tcattaaaat gatcccagtt tgcccattca
     2881 ggcactaata ctgaatttga aattaataca cgtggcgctt cttcatgtgt tttaaataca
     2941 gcaactggct ttcctgattg tactaacatt gtctcatctg attctaattc tcgtaacgtt
     3001 ttctctattg cttcaaaagc ttcccaatta cgtgctgctt ttccaatacc accataaaca
     3061 actaaatctt ctggtctttc agcaacttct gggtctaaat tgttgtataa cattctaagt
     3121 actgcttctt gttcccaacc tttacactca atactcaaac ctttttttgc ttgaattttt
     3181 ctcataaaat tcgctcctgt tcttttaaga agttaattcc actaaattta aaacgcttac
     3241 attattatct tcaatattca ttatagtatg ttaaaatata gccaacaaat ataaataaac
     3301 taattatcca tagcttgaat ctataaataa aaggagcaaa acacatgaaa attattcagt
     3361 tagaatactt cttggctatc gtgaaatata atagttttac taaagctgca caatttttac
     3421 atattagcca gccatcttta actgctacga ttaaaaaaat ggaagcagat ttaggttatg
     3481 acttatttac acgttcaaca aaagacatca agattaccga aaaaggaata cagttttatc
     3541 gttatgcgag cgaattagtt caacaatatc gatccacgat ggaaaaaatg tatgatttaa
     3601 gcgttacatc agaaccaagg ataaaaattg ggactcttga atctacgaat caatggattg
     3661 cgaatttaat tcgaaagcac cattccgact accctgaaca gcaatatcgt ttatatgaaa
     3721 tacatgataa acatcaatct atagagcaat tactgaattt taatattcat ttagctataa
     3781 caaatgaaaa aataacccac gaagatataa gatccattcc tttatatgag gaatcttaca
     3841 ttttattagc acccaaggaa acatttaaaa atcaaaattg ggtagatgtt gaaaatttgc
     3901 cactcatatt accaaacaaa aattctcaag tgcgcaaaca cttagatgac tattttaata
     3961 gaagaaatat tcgtccaaat gtcgttgtag aaacagatcg attcgaatca gcagttggat
     4021 ttgttcatct cggcttaggt tacgctatca ttccgagatt ttattaccaa tcatttcaca
     4081 cgtctaattt agaatataaa aaaattcgtc caaacttagg ccgaaaaatt tatatcaatt
     4141 accataaaaa acgcaaacac tccgaacaag tacatacatt cgtacaacaa tgccaagatt
     4201 atttatatgg acttttagag gctctttaac ttaagttatt agagcctctt atgcagttgc
     4261 gcagatcatc gtataaaaat taatgacgtc atttcaaaaa tcgatacaaa aataatttat
     4321 tataaaaatt ctaagaaaga agtgaagcag atgttaaaat ctattaatca tatatgcttt
     4381 tcagtcagaa atttaaacga ttcaatacat ttttatagag atattttact tgggaaattg
     4441 ctattgactg gtaaaaaaac tgcttatttt gagcttgcag gcctatggat tgctttaaat
     4501 gaagaaaaag atataccacg taatgaaatt cacttttcat atacacatat agctttcact
     4561 atagatgaca gcgaatttaa atattggcat cagaggttaa aagataataa cgtgaatatt
     4621 ttagaaggaa gagttagaga tattagagat agacaatcaa tttactttac cgaccctgat
     4681 ggtcataagc tagaattaca tactggcaca cttgagaaca gattaaatta ttataaagag
     4741 gctaaaccac atatgacatt ttacaaataa ggtgtcatta taaaaaggcc tcttgaactc
     4801 cgttaaaatt ttaattaatt attatataat aagagaactt ttcaaacaat acagttgtta
     4861 tttttgctat ttcaacaaac ataaataagc agtaagatga ctacaactta agagtcttc
//
LOCUS       DAKTIO010000429         4581 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.guided.190, whole genome shotgun sequence.
ACCESSION   DAKTIO010000429 DAKTIO010000000
VERSION     DAKTIO010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.guided.190"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            41..349
                     /locus_tag="O0293_003011"
                     /pseudo
     CDS             41..349
                     /locus_tag="O0293_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
     gene            349..1095
                     /locus_tag="O0293_003012"
     CDS             349..1095
                     /locus_tag="O0293_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0281799.1"
                     /translation="MNRLNTNYFDNFYIDWQNNEISENYVEFCQKQINNLSHIVPSLK
                     KEKRKIIDTCCGCGFHSKFLIDKGHHVISFDKSEEMLNFAYKKNGILGKRQSLDEEFP
                     ENHVDLILNLNTSIGYKKNDLKNLLRNSYTSLKKGGILIIDLMNPAFFYKNFEEQMIF
                     NFKDYEVNQTIKINKIYNSIVSTWDVYKNNEYSKDLSRELKFKLYTTNDIICSSLYYN
                     FTLSNISFYGDMKGNEFHENSPRLIVKITK"
     gene            1122..4196
                     /gene="mupA"
                     /locus_tag="O0293_003013"
     CDS             1122..4196
                     /gene="mupA"
                     /locus_tag="O0293_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mupirocin-resistant isoleucine--tRNA ligase
                     MupA"
                     /protein_id="HCY0281800.1"
                     /translation="MTKKYLNTQNEISAFWNTQKIFKKSIDNRKGQESFVFYDGPPTA
                     NGLPHAGHVLGRVIKDLVARLKTMQGFYVERKAGWDTHGLPVELEVEKKIGIKGKQDI
                     EKYGIENFINECKKSVFNYEKEWRDFSKDLGYWVDMDSPYITLENNYIESVWNILSTF
                     HKKGLLYKGHKVTPYCTHDQTALSSHEVAQGYKNVKDLSAVVKFQLTNSKDTYFLSWT
                     TTPWTLPANVALAINKDLNYSKIRVENEYYILATDLINSIITEKYEIIDTFSGSNLIN
                     LKYIPPFESDGLVNAYYVVDGEFVTNSEGTGIVHIAPAHGEDDYQLVLERDLDFLNVI
                     TREGVYNDRFPELVGNKAKNSDIEIIKLLSKKQLLYKKQKYEHNYPHCWRCGNPLIYY
                     AMEGWFIKTTNFKNEIINNNNNIEWFPSHIKEGRMGNFLENMVDWNIGRNRYWGTPLN
                     VWICNDCNHEYAPSSIKDLQNNSINKIDEDIELHRPYVDNITLSCPKCNGKMSRVEEV
                     IDVWFDSGSMPFAQHHYPFDNQKIFNQHFPADFIAEGVDQTRGWFYSLLVISTILKGK
                     SSYKRALSLGHILDSNGKKMSKSKGNVINPTELINKYGADSLRWALISDSAPWNNKRF
                     SENIVAQTKSKFIDTLDNIYKFYNMYNKIDHYNPNNEITKSRNTLDNWALSRLNTLIK
                     ESNIYVNNYDFTSAARLINEYTNTISNWYIRRSRGRFWEQGISNDKKDAYNTLYEILT
                     TLSRLVAPFVPFISEKIHYNLTGKSVHLQDYPQYKESFINQALEDEMHTVIKIVELSR
                     QARKNADLKIKQPLSKMVIKPNSQLNLSFLPNYYSIIKDELNIKNIELTDNINDYITY
                     ELKLNFSSVGPKLGNKTKNIQTLIDSLSEYDKKSLIESNNFKSLSSDAELTKDDFIIK
                     TLPKDSYQLSEDNDCVILLDKNLSPELIREGHARELIRLIQQLRKKKNLPINQRIDIY
                     IGVTGELLESIKTNKNMFKENLLIKNIHLNVIDEYENTIHFNNKEIKISLLY"
     gene            4266..>4581
                     /locus_tag="O0293_003014"
     CDS             4266..>4581
                     /locus_tag="O0293_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCY0281801.1"
                     /translation="MYNFDYSKLPIKNIQKIFPIAGGYVNLSFSVDASNKKYFLKLQP
                     NTKSNFFDYELSSLKELTDKNIPVPQIINKGELDNNSFLLLEFIENGHAYPESYRKLG
                     KIV"
BASE COUNT         1812 a          571 c          702 g         1496 t
ORIGIN      
        1 tgtatagatg tgcatataat tttaactttg caacagaacc gaaataacta ttccatttct
       61 aacaaataat actgatagtt ctaattatat ttttgttata tttgtaaaaa atcctatttt
      121 gaaagaaaaa atgattactg atcttagaaa tgagaagata cagactagtt tccattatac
      181 tcccttacat aaatttagtt attataaatc aaaaaactta ttaccaattg tagaggaggt
      241 ttctaaaggt ttagttacat tacccttaca taatcgttta tctatcaatg atgtaaaaag
      301 ggtctgcact attattagta aaagtataca ggaatggaga gagcaataat gaatcggcta
      361 aatacaaatt actttgataa cttttatatt gattggcaga acaatgaaat atcagaaaat
      421 tatgttgaat tttgtcaaaa gcaaattaat aacttaagtc acattgttcc ttcattaaaa
      481 aaagagaaaa gaaaaatcat cgatacttgt tgtggctgtg gttttcattc taaatttttg
      541 atagataaag gtcatcatgt tattagcttt gacaaatctg aggaaatgtt gaactttgca
      601 tataaaaaaa atggcatttt aggtaaaaga caatctttag atgaagaatt cccagaaaat
      661 cacgtcgact taattcttaa tttaaatact tctatcggat ataaaaaaaa tgatttaaaa
      721 aatttgttaa gaaatagtta tacctcctta aaaaaaggtg gtattttaat tatagatttg
      781 atgaaccccg cctttttcta taaaaatttt gaagaacaaa tgattttcaa cttcaaagat
      841 tacgaagtta atcaaactat taaaataaat aaaatttata atagtatagt ttcaacatgg
      901 gatgtttata aaaataacga atatagcaaa gacctttcaa gagaattaaa atttaaatta
      961 tatactacta atgatataat ttgcagtagt ttgtattata actttacttt atccaatata
     1021 tctttctatg gagacatgaa aggaaatgaa tttcatgaaa attcaccaag actaatagta
     1081 aaaataacga aataagtgat actctaggag gctgaaaagt tttgacaaag aaatatttaa
     1141 acacccagaa tgaaatatca gcattttgga atactcaaaa gatatttaaa aaatcaattg
     1201 acaatagaaa aggacaggaa agttttgttt tttatgacgg ccccccaact gcaaatggcc
     1261 ttcctcatgc tggccatgtt cttggaagag taatcaagga tttagttgca agattaaaaa
     1321 ctatgcaagg tttttatgta gaaagaaaag caggatggga tacccatggc ttaccagttg
     1381 aattagaggt tgaaaaaaaa attggaatta aaggaaaaca agacattgaa aagtatggaa
     1441 tagaaaattt tataaatgaa tgtaaaaaaa gtgtatttaa ttatgaaaaa gaatggcggg
     1501 atttttctaa agatttagga tactgggttg acatggactc cccctatata actcttgaga
     1561 ataattatat tgaaagtgta tggaatatat tatctacatt ccataaaaaa ggactattat
     1621 ataagggaca taaggtgact ccttattgta cacatgatca aaccgcttta agttctcatg
     1681 aagtagcgca aggctataaa aacgttaaag atttatcagc tgttgttaaa tttcaactta
     1741 caaatagtaa agatacttat ttcttaagtt ggactaccac tccctggact ttgcctgcaa
     1801 atgtagcatt agctataaat aaagatctta attattcaaa aattcgggta gaaaatgagt
     1861 attatatctt agctacagat ctaattaatt ctataataac tgaaaaatac gaaattattg
     1921 ataccttttc aggaagtaat ttaattaatt taaaatacat tcctcctttt gaaagcgacg
     1981 gtttagttaa tgcatattac gttgttgatg gagaatttgt tactaactca gaaggaactg
     2041 gtattgttca tatagcacca gctcatgggg aagatgacta ccaattggtt ttagagcgtg
     2101 atttggattt cttaaatgtt ataacaagag aaggagtata taatgatagg ttccctgaat
     2161 tagttggtaa taaagctaaa aatagtgata tagaaatcat aaaattatta tccaaaaaac
     2221 aacttttata taaaaaacaa aaatatgagc ataattatcc tcattgttgg agatgtggta
     2281 atcctttgat atattatgcg atggaaggtt ggtttattaa aacaactaat tttaagaatg
     2341 aaattattaa caataataat aatatagagt ggtttccttc tcatattaag gaagggagaa
     2401 tgggaaattt cttagaaaat atggttgatt ggaacattgg tagaaataga tattggggaa
     2461 caccattaaa tgtatggatt tgcaatgatt gtaatcacga atacgcacca agtagtatta
     2521 aggatttaca aaataattcc atcaataaaa ttgatgaaga tattgagttg catagacctt
     2581 atgttgataa tatcactctt agttgcccta agtgtaatgg gaaaatgtct cgagtagaag
     2641 aagtaatcga tgtttggttt gatagcggct ctatgccgtt tgctcagcat cattatcctt
     2701 ttgataacca gaaaattttt aatcaacact ttccagctga ttttattgca gaaggagttg
     2761 atcaaacgag aggctggttt tacagtttac tagtaatttc tactattcta aaaggaaaat
     2821 cttcttataa acgtgcttta tctttaggac atattctaga cagtaatggt aaaaaaatgt
     2881 ctaaaagtaa aggaaacgtt attaatccaa ctgaattaat taataagtac ggagccgatt
     2941 ctttaagatg ggccttaatt tcggatagtg ctccatggaa taacaaaaga ttctcagaaa
     3001 atatagtagc tcagaccaaa tcgaaattta tagatacgct tgataatatt tataaatttt
     3061 ataatatgta taataaaata gatcactata atcctaataa tgaaattaca aaaagtagaa
     3121 atacattaga taattgggct ctttctcgct taaacacctt aataaaagaa agtaatattt
     3181 atgtaaataa ttacgatttc acttccgcag ccagattaat taacgaatat accaatacaa
     3241 taagtaattg gtatattcgg agatcgagag gacgattttg ggaacaagga atttctaacg
     3301 ataaaaaaga tgcgtacaat acgctttatg aaattttaac aactttatca agactagtgg
     3361 ctccatttgt tccatttata tctgaaaaaa tccattataa tttgactgga aaaagtgtgc
     3421 atttacaaga ttatccacaa tataaagaaa gttttattaa tcaagcattg gaagatgaaa
     3481 tgcataccgt tataaaaatt gtagaattat ctagacaggc tcgcaaaaat gcagatttaa
     3541 aaattaagca acctttatcg aaaatggtga ttaaacctaa tagtcaatta aacttaagtt
     3601 ttttacctaa ttactattca ataataaaag acgaattaaa tataaaaaac attgaattaa
     3661 ctgataatat taatgactat attacctatg agcttaaatt gaatttttct tctgtgggac
     3721 caaaactagg gaacaaaacg aaaaatattc aaacattgat agactcccta tcagagtatg
     3781 ataaaaaaag tttaattgag tctaataact tcaaaagttt atcttctgat gctgagttaa
     3841 ctaaggatga ttttataatt aaaaccttac ctaaggatag ttatcaactc agtgaagata
     3901 atgactgcgt tatattatta gataaaaatt tatctcctga attaattcgc gaaggacatg
     3961 ctagagagct cattagatta attcaacaat taagaaaaaa gaaaaattta ccaataaatc
     4021 aacgtattga tatttatatc ggtgtaactg gggaattatt agaatcaata aaaaccaata
     4081 aaaatatgtt taaagaaaat ttgctgatta aaaatataca cttaaatgtt atagatgaat
     4141 atgaaaatac tattcatttt aataataaag aaataaaaat ttccttatta tattaaacaa
     4201 acatggccac tctattttag tagagtggcc atgagttatc aaagccaagc aaaaggtgat
     4261 aaactatgta taattttgat tattctaaat taccaattaa aaatattcaa aaaatatttc
     4321 ctattgcagg gggatatgtg aatctatctt tttcagttga tgcttccaac aaaaaatatt
     4381 ttttaaagct acaacctaat actaaatcaa atttttttga ttatgagtta tccagtttaa
     4441 aagaattaac tgataaaaat attcctgttc ctcaaattat taataaagga gaattagaca
     4501 ataattcttt tttactgtta gagttcattg aaaatggcca tgcttatcct gaaagctata
     4561 gaaaactagg taaaatagtt g
//
LOCUS       DAKTIO010000430         3299 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.guided.225, whole genome shotgun sequence.
ACCESSION   DAKTIO010000430 DAKTIO010000000
VERSION     DAKTIO010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.guided.225"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            39..416
                     /locus_tag="O0293_003015"
                     /pseudo
     CDS             39..416
                     /locus_tag="O0293_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            413..1294
                     /locus_tag="O0293_003016"
                     /pseudo
     CDS             413..1294
                     /locus_tag="O0293_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1410..2339
                     /locus_tag="O0293_003017"
     CDS             1410..2339
                     /locus_tag="O0293_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0281802.1"
                     /translation="MKNLNNIIEINNLTKQYGSQIAVNNLSITVESGQIYGFLGRNGA
                     GKTTTIRMLLGLIKPTKGNIKIFEKEFESNKTEILKNIGAMVETPGFYSNLTAKENLT
                     INAKILGIQKENAIEEVLEIVGLASEKVKLFKNYSLGMKQRLGIARALLHNPELLILD
                     EPTNGLDPIGIKEIRKLIKDLAQSRNITILISSHILSEIEQLVDKVGIIHNGVLLEEI
                     MYEDLKKKNRQFISISVSDEEKVACLLETNFGIYDYKIKENGEFKIYSHIDRQQEINR
                     LLVLNNIDVFQMRVIEDNLETYFEDLVGGGTIG"
     gene            2332..3069
                     /locus_tag="O0293_003018"
     CDS             2332..3069
                     /locus_tag="O0293_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0281803.1"
                     /translation="MDNLLRTEFIKLKRSYMFLISILGTCVAPFMVVVASFISMRTKV
                     PTPNIVFKQLFEEVSLYHMLIIGPPLYGVIIAYLFGREYIEDTLNNLLIIPISRESFL
                     VSKIILLFLWIQTLSVIAWLLTLMLGMLLHFQGLSQSLIIDSFFTFVIGGVLLWILSP
                     PIIILSIYFKNYVPTIVLTIIITLINLMTANSEHRGLFPWAAVGDISRGTLPNTYPFL
                     LSLIIIIVTSIFSVIILFSYFKKTDIS"
     gene            3083..3286
                     /locus_tag="O0293_003019"
     CDS             3083..3286
                     /locus_tag="O0293_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008406263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0281804.1"
                     /translation="MGFIEQIKAFILGVVEGLTEFAPVSSTGHMIIVDDMWLNTADDL
                     GSASATTFKVVIQLGGSVAKLNL"
BASE COUNT         1200 a          433 c          587 g         1079 t
ORIGIN      
        1 attagtgatt agctatattt tttactttgc aacagaacca gccatttaat atgactgtta
       61 caattactaa gattcaagca ctattacgcc gcacgtatga tttcaccact caagttaata
      121 atttaacagt agatgattgc gagttactac ttgatgcagc aaagcttaaa tttaatgaag
      181 aaaccataga cctgacacac actgaattgc aaatcatgca tagcttattc cagaacaagg
      241 gcaaattcgt cagtcgtaat gcgttaatcg aaaaatgctg ggaatcagaa cacttcattg
      301 atgacaatac gcttgctgtc aacattacac gtttacgcaa aaagctacaa aaactaggac
      361 ttacagattt tatcgaaacg aagaaaaatg tcggatatcg tgtaggtgac gtatgaattt
      421 cttgaaatca atccaacgtg aaatttatat tgtcatcctt acttgtgctt tattctactt
      481 tatattattc atgtataatg tgccgatgca agcatatatg attggtctag gtattgtatt
      541 gtttgtttca atgatttact ggggtatcca ttacatgaac tttaaaaaag aataaacact
      601 acaggaacag aacgaagatt taagaatcga aaaccataaa cttcgtaatg actttattaa
      661 ctaccaaagt gagttagagt catatttttt aatgtgggtg catcaaatca agacaccgat
      721 tacagcatca aagttactgc ttgaagatcc tgatgaacac gtgatttcta acgtgcgtaa
      781 tgaagtctta caaattgata attacacgaa tcttgcactg aactatttga aactcatgaa
      841 ccaaaagaca tacatggtct ttatggaagt gagtgtagat gaattaattc gaccactgat
      901 taaacgttat gcgatacagt ttattcatca tcaaacaaag ttgcattatg agaaaagtga
      961 agcggttata ttaacagatg ccaaatgggc atcaattatg attgagcaaa tattaaacaa
     1021 tgcattaaaa tatgcacgtg gcaaagatgt gtggattacg tttgacgaaa atacaaagaa
     1081 attatcaatc cgagataacg gtatcggcat taatgcgagt gatttaccga aaatctttga
     1141 taaaggtttc tcgggctata acggtagaat aagtgagaaa tcaagcggta ttggtttata
     1201 tattgtcagc acaatctcaa agcgcttagg ccataaagta agtgctacat ctacattaaa
     1261 cgatggttca atattcacca ttcaatttaa ataactccaa tctttcattt ttgtaagatt
     1321 agcggctgtt tttgtaagaa tagataaagg cagccttttc taatcgcgcg taagataaag
     1381 gtaacaacaa actaagaaag gtgccagagg tgaaaaattt gaataatata attgaaataa
     1441 ataacttaac aaagcaatat ggaagtcaga tagcagttaa taatttgagt ataactgttg
     1501 aaagtggaca aatttatggt tttttaggaa gaaatggtgc aggcaaaaca actactataa
     1561 gaatgctttt aggcttaatt aaacctacga aaggtaatat taaaatattt gaaaaagagt
     1621 tcgaaagtaa taaaactgaa attttaaaaa atattggtgc tatggttgaa acgccaggtt
     1681 tttatagtaa tttgacagct aaagagaact taactataaa tgctaaaata ttaggtatac
     1741 aaaaagaaaa tgcaattgaa gaagtgttag aaatagtagg actagcttct gaaaaagtaa
     1801 aattgtttaa aaattattct ttaggtatga agcaacgttt agggattgcg agagctttat
     1861 tgcacaatcc cgaattattg atacttgatg agccgacgaa tggattagat cctattggca
     1921 taaaagaaat tagaaagttg attaaggatc tggctcaatc tagaaatata accattttaa
     1981 tttcaagtca tatattgtca gaaatagagc agttagtaga caaggtgggt attattcata
     2041 acggagtttt gttagaagaa attatgtatg aagatttaaa aaaaaagaac agacaattta
     2101 taagtatatc agtatcagat gaagaaaaag ttgcatgttt gttggaaact aacttcggaa
     2161 tttatgacta taaaattaaa gaaaatggcg aatttaaaat ttattcacat attgatagac
     2221 aacaagaaat taatagatta ttagtattaa acaatattga tgttttccag atgagagtga
     2281 tagaagataa tctagaaact tatttcgaag acttagtagg aggtggcaca attggataat
     2341 ctactccgta cagaatttat taagttaaaa agatcatata tgtttctaat aagtatttta
     2401 ggtacatgcg tagcaccttt tatggtggtt gtagcttctt ttattagtat gcggacgaaa
     2461 gtaccgactc ctaatattgt atttaaacag ttatttgaag aagtcagttt atatcatatg
     2521 ctaattattg gacctcctct atatggggtt ataatagcct atttgtttgg aagagaatat
     2581 atagaagata ctttaaataa cttattaatt atacctatat ctagagaaag ttttttagta
     2641 agtaaaataa tattattatt cttgtggatt caaacactat ctgttattgc atggttgcta
     2701 actttaatgc taggtatgct attgcatttt caagggttaa gccagagttt aataattgat
     2761 tcattcttta catttgttat tggcggagtg ttattatgga tattatctcc accaataatt
     2821 attttgtcga tatattttaa aaattatgtt cccacaattg ttttaaccat aataattact
     2881 ttgataaatt taatgactgc taattctgaa catagaggat tatttccttg ggctgcagtt
     2941 ggagacatat ctagaggaac attacctaat acttatccat ttctattatc tttaattatc
     3001 ataattgtta catctatatt cagtgtaatt atattattct catactttaa aaaaactgat
     3061 ataagttaaa aggaggagaa aaatgggttt tatagagcag attaaagcat ttattttagg
     3121 tgtagttgaa gggttgacag aatttgctcc agtatcatca acaggtcata tgattattgt
     3181 tgatgatatg tggttaaaca ctgctgacga tttaggaagt gcgtctgcaa ctacttttaa
     3241 ggtggtaata cagttaggag gttctgttgc aaagttgaat ttataatata attttaaca
//
LOCUS       DAKTIO010000431         3167 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.guided.231, whole genome shotgun sequence.
ACCESSION   DAKTIO010000431 DAKTIO010000000
VERSION     DAKTIO010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.guided.231"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            122..757
                     /locus_tag="O0293_003020"
                     /pseudo
     CDS             122..757
                     /locus_tag="O0293_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            754..1284
                     /gene="sat4"
                     /locus_tag="O0293_003021"
     CDS             754..1284
                     /gene="sat4"
                     /locus_tag="O0293_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HCY0281805.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFEKAVFWYLRF"
     gene            1377..2171
                     /locus_tag="O0293_003022"
                     /note="aph(3')-IIIa"
     CDS             1377..2171
                     /locus_tag="O0293_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCY0281806.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            2447..3018
                     /locus_tag="O0293_003023"
                     /pseudo
     CDS             2447..3018
                     /locus_tag="O0293_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
BASE COUNT         1065 a          497 c          722 g          883 t
ORIGIN      
        1 attagtgatt agctatattt tttactttgc aacagaacca tcatttttat taaaatactc
       61 ttttgtaaaa tctttctgct cttcaatgta tttaatttct ttacctctaa atctattata
      121 gctatttgat gattacaata aaattgatct taccttattg cccttggaag agttagataa
      181 ttacctaaag ggcgataaat taataaaggt tctaattgat aaagattgta gaattaaaag
      241 ggacatagtt ccgactgata tagattatca tgtaagaaag ccaagcgcaa gggagtatga
      301 tgattgctgc aatgaatttt ggaatgtaac accttatgtt attaaaggat tgtgccgtaa
      361 ggaaatttta tttgctattg atcattttaa tcagattgtt cgccatgagc tgctgagaat
      421 gatatcatgg aaggtcggca tcgaaacagg ctttaaatga agtgtaggca agaactataa
      481 gtttattgaa aggtatatat ccgaggattt gtgggagaaa cttttgtcca cctaccggat
      541 ggattcctat gaaaacatat gggaagcatt atttctatgc catcaattgt tcagggcggt
      601 atccggtgag gtggcggaaa ggcttcatta tgcctatccg gagtatgata ggaatataac
      661 aaaatatacc agggacatgt ataaaaaata cactggtaaa accggctgcc tggatagcac
      721 atatgccgct gatatagaag agaggcggga acagtgatta cagaaatgaa agcagggcac
      781 ctgaaagata tcgataaacc cagcgaacca tttgaggtga taggtaagat tataccgagg
      841 tatgaaaacg agaattggac ctttacagaa ttactctatg aagcgccata tttaaaaagc
      901 taccaagacg aagaggatga agaggatgag gaggcagatt gccttgaata tattgacaat
      961 actgataaga taatatatct ttactaccaa gacgataaat gcgtcggaaa agttaaactg
     1021 cgaaaaaatt ggaaccggta cgcttatata gaagatatcg ccgtatgtaa ggatttcagg
     1081 gggcaaggca taggcagcgc gcttatcaat atatctatag aatgggcaaa gcataaaaac
     1141 ttgcatggac taatgcttga aacccaggac aataacctta tagcttgtaa attctatcat
     1201 aattgtggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt tgaaaaagct
     1261 gttttctggt atttaaggtt ttagaatgca aggaacagtg aattggagtt cgtcttgtta
     1321 taattagctt cttggggtat ctttaaatac tgtagaaaag aggaaggaaa taataaatgg
     1381 ctaaaatgag aatatcaccg gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag
     1441 atacggaagg aatgtctcct gctaaggtat ataagctggt gggagaaaat gaaaacctat
     1501 atttaaaaat gacggacagc cggtataaag ggaccaccta tgatgtggaa cgggaaaagg
     1561 acatgatgct atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc
     1621 atgatggctg gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt
     1681 atgaagatga acaaagccct gaaaagatta tcgagctgta tgcggagtgc atcaggctct
     1741 ttcactccat cgacatatcg gattgtccct atacgaatag cttagacagc cgcttagccg
     1801 aattggatta cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag
     1861 acactccatt taaagatccg cgcgagctgt atgatttttt aaagacggaa aagcccgaag
     1921 aggaacttgt cttttcccac ggcgacctgg gagacagcaa catctttgtg aaagatggca
     1981 aagtaagtgg ctttattgat cttgggagaa gcggcagggc ggacaagtgg tatgacattg
     2041 ccttctgcgt ccggtcgatc agggaggata tcggggaaga acagtatgtc gagctatttt
     2101 ttgacttact ggggatcaag cctgattggg agaaaataaa atattatatt ttactggatg
     2161 aattgtttta gtacctagat ttagatgtct aaaaagcttt aactacaagc tttttagaca
     2221 tctaatcttt tctgaagtac atccgcaact gtccatactc tgatgtttta tatcttttct
     2281 aaaagttcgc tagatagagt tctatattag agatgtaaag agttttggtg aagaggtttg
     2341 tgagtatggc ttttataacc agggtgcaat gagaagcact aacagcagct agcttaagaa
     2401 cgcataggat atacttttta tggaagtcac agaggaggaa taaaatttga acaggattaa
     2461 cagagtaacc tctattctta ttcagctgca atcaaaaaaa taattcctgc caaagaaatt
     2521 gcacagcgat ttaatataag cttaaggaca gtttacagag atatccggac acttgaagaa
     2581 gccggaatac caattggatc tgaggccgga aaaggatatt ttcttgtaga gggcttccta
     2641 cttctgccgg taatgtttac agcggcggaa gtgggtgcat tgattacagc agggaaattt
     2701 ttaaattgcc atggagatga atcatttata aaggattttg attcagctat gtataaaatc
     2761 aaatctattt tgaagcatgg tgaaaaaaac tatgcacagg agttggagaa cagtattaac
     2821 gtgtacagca catctggaca gaaaaataca ttggcggata acgttattgc agcaattcag
     2881 actgcaatct gcaataaaag ggtaatatca attcaatatc ctgcatcagg agggcaagaa
     2941 ccggaaagca gaatgataga gcctatatca ctggggtttt atgaacagaa ctggtactta
     3001 atcggttttg caggctaaga aatgaatata ggaattttcg tgtggatcgg attaaaagca
     3061 tttgcataga agaagagctc tgtcaaagcc atgatggctc tttggaacaa atattaaaac
     3121 aaatgctggt tctgttgcaa agtaaaaaaa tatagctaac cactaat
//
LOCUS       DAKTIO010000432         2399 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.guided.261, whole genome shotgun sequence.
ACCESSION   DAKTIO010000432 DAKTIO010000000
VERSION     DAKTIO010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.guided.261"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            192..899
                     /gene="deoD"
                     /locus_tag="O0293_003024"
     CDS             192..899
                     /gene="deoD"
                     /locus_tag="O0293_003024"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCY0281807.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            906..2258
                     /gene="tet(38)"
                     /locus_tag="O0293_003025"
     CDS             906..2258
                     /gene="tet(38)"
                     /locus_tag="O0293_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HCY0281808.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFPLL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVSLNRWTQSEK
                     "
BASE COUNT          774 a          347 c          436 g          842 t
ORIGIN      
        1 ttggaaaaat tataaaaatt aaatatagat aggctttgaa aattagtttt atacaaggtt
       61 agtagctgta actgtaaaat gttcttaata ttgtcaaaat gtaatgcttg aaagcgcttt
      121 taaaaaatat tattatatac atggttagac aaatagacaa atcactatac aaatattggg
      181 aggaatattt tatgaaatca acaccacaca ttaaaccaat gaatgacgtc gaaattgcag
      241 aaacggttct attgccagga gatccgttaa gagctaagtt cattgcagaa acttatttgg
      301 atgatgtgga acagttcaat acagtgcgaa acatgtttgg ttttaccgga acatataaag
      361 gtaaaaaagt ttctgtcatg ggttcaggta tgggtatgcc atctattggc atttactctt
      421 atgaattaat tcatacattt ggttgtaaaa aattaattcg cgttggctct tgtggcgcga
      481 tgcaagaaaa cattgattta tatgatgtga ttattgcaca aggtgcctct actgattcaa
      541 attacgttca acaatatcaa ttaccaggtc attttgcgcc aattgcttct tatcaattat
      601 tagaaaaagc agttgaaaca gcacgtgaca aaggtgtacg tcatcatgta ggtaatgtgt
      661 tatcaagtga tattttctat aacgcggata caacagcgag tgaacgttgg atgcgtatgg
      721 gtattttagg tgtagaaatg gaatcagctg cattatacat gaatgcaatt tacgctggtg
      781 tcgaagcatt aggtgtgttc acagtgagcg atcatttaat tcatgaaacg tcaacaacac
      841 ctgaggaaag ggaacgtgca tttacagata tgattgaaat tgcactgtca ttggtgtaga
      901 tgattatgaa tgttgaatat tctaaaataa agaaagcagt acctatttta ttattcttat
      961 ttgtattcag tttggttata gacaactcat ttaaattgat ttctgtagcc attgctgatg
     1021 acttaaacat atctgtaacg acagtaagtt ggcaagcgac attagccggt ttagtaattg
     1081 gtattggcgc tgtagtatac gcttcattat ctgatgccat tagtatacgc acactattta
     1141 tttatggcgt gatattaatc attatcggat caattattgg ttacattttc caacatcaat
     1201 tcccattact tttagttgga cgtattattc aaactgccgg tttagctgct gcagagacat
     1261 tatatgtgat atatgttgca aagtatcttt ctaaagagga ccagaagact taccttggct
     1321 taagtacgag cagttattcc ttgtcattag ttatcggtac attatcaggt ggatttattt
     1381 ctacgtattt acactggaca aatatgtttt taattgcatt aatcgtagta tttacgttgc
     1441 cattcctatt taaattatta ccaaaagaaa ataatacgaa taaagctcat ttagattttg
     1501 ttggcttaat tctagtggca actattgcta caacagtcat gctgtttatt acgaacttta
     1561 attggttata tatgattggt gccttaattg cgattatcgt ttttgcgcta tatattaaaa
     1621 atgcgcaacg tccattagta aataaatcat ttttccaaaa taaacgttat gcttcatttt
     1681 tatttatagt atttgtaatg tatgctatcc aattgggtta tatttttacg ttcccattca
     1741 taatggagca aatttatcat ctgcaactag acacaacatc actgttatta gtaccgggtt
     1801 atatagtagc agtcattgtt ggtgcactaa gtggtaaaat cggcgaatat ctgaattcaa
     1861 aacaagcgat tatcacagca attattttaa tagcactgag cttgatttta cctgcatttg
     1921 cagtaggtaa tcacatttca atcttcgtca tttctatgat attctttgca ggtagctttg
     1981 ctttaatgta tgcaccttta cttaacgaag ccattaaaac aatagatctt aatatgacag
     2041 gtgtggctat tggtttttat aatttaatta ttaatgtggc ggtatctgta ggtattgcga
     2101 ttgctgcggc tctaatcgat tttaaagcat taaatttccc aggcaatgat gcattaagtt
     2161 cacatttcgg tattatttta attattttag gtttaatgag tattgtcgga ttagttttat
     2221 tcgtcagctt aaatcgttgg acacaatctg aaaaataaat agatataaat tcgcgagata
     2281 tattcgtatt tatagtaaaa ttaaataaag agattatata acacgaggag tagtaagtat
     2341 gaaatttgag aaatatatag atcacacttt attgaagcct gagtcaacac gtacgcaaa
//
LOCUS       DAKTIO010000433         1998 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.guided.281, whole genome shotgun sequence.
ACCESSION   DAKTIO010000433 DAKTIO010000000
VERSION     DAKTIO010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.guided.281"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            47..439
                     /locus_tag="O0293_003026"
     CDS             47..439
                     /locus_tag="O0293_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0281809.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            440..1879
                     /locus_tag="O0293_003027"
                     /note="aph(2'')-Ia"
     CDS             440..1879
                     /locus_tag="O0293_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HCY0281810.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          831 a          153 c          343 g          671 t
ORIGIN      
        1 aacattgtat agcattttac acaggagtct ggacttgact gagtttatgg aagaagtttt
       61 aattgatgat aatatggttt ttgatattga taatttaaaa ggatttctta atgataccag
      121 ttcatttggg tttatagcta aagaaaataa taaaattata ggatttgcat attgctatac
      181 acttttaaga cctgatggaa aaacaatgtt ttatttacac tcaataggaa tgttacctaa
      241 ctatcaagac aaaggttatg gttcaaaatt attatctttt attaaggaat attctaaaga
      301 gattggttgt tctgaaatgt ttttaataac tgataaaggt aatcctagag cttgccatgt
      361 atatgaaaaa ttaggtggta aaaatgatta taaagatgaa atagtatatg tatatgatta
      421 tgaaaaaggt gataaataaa tgaatatagt tgaaaatgaa atatgtataa gaactttaat
      481 agatgatgat tttcctttga tgttaaaatg gttaactgat gaaagagtat tagaatttta
      541 tggtggtaga gataaaaaat atacattaga atcattaaaa aaacattata cagagccttg
      601 ggaagatgaa gtttttagag taattattga atataacaat gttcctattg gatatggaca
      661 aatatataaa atgtatgatg agttatatac tgattatcat tatccaaaaa ctgatgagat
      721 agtctatggt atggatcaat ttataggaga gccaaattat tggagtaaag gaattggtac
      781 aagatatatt aaattgattt ttgaattttt gaaaaaagaa agaaatgcta atgcagttat
      841 tttagaccct cataaaaata atccaagagc aataagggca taccaaaaat ctggttttag
      901 aattattgaa gatttgccag aacatgaatt acacgagggc aaaaaagaag attgttattt
      961 aatggaatat agatatgatg ataatgccac aaatgttaag gcaatgaaat atttaattga
     1021 gcattacttt gataatttca aagtagatag tattgaaata atcggtagtg gttatgatag
     1081 tgtggcatat ttagttaata atgaatacat ttttaaaaca aaatttagta ctaataagaa
     1141 aaaaggttat gcaaaagaaa aagcaatata taatttttta aatacaaatt tagaaactaa
     1201 tgtaaaaatt cctaatattg aatattcgta tattagtgat gaattatcta tactaggtta
     1261 taaagaaatt aaaggaactt ttttaacacc agaaatttat tctactatgt cagaagaaga
     1321 acaaaatttg ttaaaacgag atattgccag ttttttaaga caaatgcacg gtttagatta
     1381 tacagatatt agtgaatgta ctattgataa taaacaaaat gtattagaag agtatatatt
     1441 gttgcgtgaa actatttata atgatttaac tgatatagaa aaagattata tagaaagttt
     1501 tatggaaaga ctaaatgcaa caacagtttt tgagggtaaa aagtgtttat gccataatga
     1561 ttttagttgt aatcatctat tgttagatgg caataataga ttaactggaa taattgattt
     1621 tggagattct ggaattatag atgaatattg tgattttata tacttacttg aagatagtga
     1681 agaagaaata ggaacaaatt ttggagaaga tatattaaga atgtatggaa atatagatat
     1741 tgagaaagca aaagaatatc aagatatagt tgaagaatat tatcctattg aaactattgt
     1801 ttatggaatt aaaaatatta aacaggaatt tatcgaaaat ggtagaaaag aaatttataa
     1861 aaggacttat aaagattgat tatataatat atgaaaagct attataaaag acattagtat
     1921 taaatagttt aaaaaaatga aaaataataa aggaagtgag tcaagtccag actcctgtgt
     1981 aaaatgctat acaatgtt
//
LOCUS       DAKTIO010000434         1802 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.guided.296, whole genome shotgun sequence.
ACCESSION   DAKTIO010000434 DAKTIO010000000
VERSION     DAKTIO010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.guided.296"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..665
                     /locus_tag="O0293_003028"
     CDS             <1..665
                     /locus_tag="O0293_003028"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCY0281811.1"
                     /translation="YKGPDMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQF
                     GDLGNVYGKQWRDWVDKDGNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPP
                     CHTMFQFYVQDGKLSCQLYQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTF
                     GDAHIYSNHIDAIQTQLARESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPI
                     AV"
     gene            865..1344
                     /locus_tag="O0293_003029"
     CDS             865..1344
                     /locus_tag="O0293_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCY0281812.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            1359..>1802
                     /gene="fakB2"
                     /locus_tag="O0293_003030"
     CDS             1359..>1802
                     /gene="fakB2"
                     /locus_tag="O0293_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HCY0281813.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNH"
BASE COUNT          651 a          278 c          319 g          554 t
ORIGIN      
        1 agtataaagg tccagatatg acagatttcg ggcatcgtgc attgagtgat cctgaattta
       61 acgaacaata taaagaacaa atgaaacaat ttaagcaacg tattcttgaa gatgatacat
      121 ttgcgaagca attcggggat ttaggaaatg tttatggtaa acaatggcga gattgggttg
      181 ataaagatgg taatcatttt gatcaactta aaacagtaat tgaacaaatt aagcataatc
      241 cagattcaag gcgacacatc gtatctgcat ggaatccaac agaaattgat acaatggcac
      301 ttccgccttg tcataccatg ttccagtttt atgtccaaga tggtaagtta agttgccagt
      361 tataccaacg tagcgcagat atctttttag gtgtgccatt taatatcgca agctacgctt
      421 tattgacaca ccttattgcc aaagaatgtg gacttgaagt gggtgaattt gtgcatacat
      481 ttggagatgc acatatttat tcaaatcata ttgatgcgat tcaaacacaa ttagcacgtg
      541 aaagcttcaa tcctccaaca ttaaaaatta acagtgacaa gtctattttc gacataaatt
      601 atgaagattt ggaaattgtt gactatgaat cacatccagc aataaaagct ccaatagcag
      661 tgtagtcatt gcatagttag ctaaccatat agacatcaaa atgacatcat agtattttca
      721 agtgcaaaaa agtacttttt tgtgttaaac gttttcataa attatgcaaa atcattattt
      781 ctatcacact ttatgataaa aattgtgtta aattaaagat aacttagtaa taaaaaatga
      841 aatgatagaa gaaggaggat aattatgact ttatccattc tagttgcaca tgacttgcaa
      901 cgagtaattg gttttgaaaa tcaattacct tggcacctac caaatgattt gaagcatgtt
      961 aaaaaattat caacaggtca tactttagta atgggtcgta agacatttga atcgattggt
     1021 aaaccactac cgaatcgtcg aaatgttgta cttacttcag atacaagttt caacgtagag
     1081 ggcgttgatg taattcactc tattgaagat atttaccaac taccgggcca tgttttcata
     1141 tttggagggc aaacattatt tgaagaaatg attgataaag tggacgacat gtatattact
     1201 gttattgaag gtaaattccg tggtgatacg ttctttccac cttatacatt tgaagactgg
     1261 gaagttgcct cttcagttga aggtaaacta gatgagaaaa atacaattcc acataccttt
     1321 ctacatttaa ttcgtaaaaa ataaggggga aaacgaccat gacaaaacag attatagtaa
     1381 cagactcaac atccgattta tctaaagaat acttagaagc aaacaacatt catgtaattc
     1441 ctttaagttt aactattgaa ggagcttcat acgttgacca agtagatatt acatcagaag
     1501 aatttattaa tcatattgaa aatgatgaag atgtaaagac aagtcagcca gccataggtg
     1561 aatttatatc tgcttatgaa gaactaggaa aagatggctc tgaaatcata agtattcatc
     1621 tttcttcagg attaagtggt acatataaca ctgcttacca agcaagtcaa atggtagatg
     1681 ctaatgtaac tgttattgat tcaaaatcta tttcttttgg tttagggtat caaatacaac
     1741 acctagtaga gcttgtaaaa gaaggtgtct caacttctga aatagttaaa aagttaaatc
     1801 at
//
LOCUS       DAKTIO010000435         1356 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.guided.328, whole genome shotgun sequence.
ACCESSION   DAKTIO010000435 DAKTIO010000000
VERSION     DAKTIO010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.guided.328"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            73..171
                     /locus_tag="O0293_003031"
     CDS             73..171
                     /locus_tag="O0293_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCY0281814.1"
                     /translation="MIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     gene            331..1101
                     /gene="aadD1"
                     /locus_tag="O0293_003032"
     CDS             331..1101
                     /gene="aadD1"
                     /locus_tag="O0293_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-nucleotidyltransferase
                     ANT(4')-Ia"
                     /protein_id="HCY0281815.1"
                     /translation="MRIVNGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLG
                     RQTDGPYSDIEMMCVMSTEEAEFSHEWTTGEWKVEVNFDSEEILLDYASQVESDWPLT
                     HGQFFSILPIYDSGGYLEKVYQTAKSVEAQTFHDAICALIVEELFEYAGKWRNIRVQG
                     PTTFLPSLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQSDLPSGYDHLCQFVMSG
                     QLSDSEKLLESLENFWNGIQEWTERHGYIVDVSKRIPF"
BASE COUNT          449 a          197 c          311 g          399 t
ORIGIN      
        1 taaaattata ctataaattc aactttgcaa cagaaccaaa aattaaacct tgatgacaca
       61 gaagaaggcg atttgattca tacagatgat gacgaaaaag ccgatgaaga tggattttct
      121 attattgcaa tgtggaattg ggaacggaaa aattatttta ttaaagagta gttcaacaaa
      181 cgggccagtt tgttgaagat tagatgctat aattgttatt aaaaggattg aaggatgctt
      241 aggaagacga gttattaata gctgaataag aacggtgctc tccaaatatt cttatttaga
      301 aaagcaaatc taaaattatc tgaaaaggga atgagaatag tgaatggacc aataataatg
      361 actagagaag aaagaatgaa gattgttcat gaaattaagg aacgaatatt ggataaatat
      421 ggggatgatg ttaaggctat tggtgtttat ggctctcttg gtcgtcagac tgatgggccc
      481 tattcggata ttgagatgat gtgtgtcatg tcaacagagg aagcagagtt cagccatgaa
      541 tggacaaccg gtgagtggaa ggtggaagtg aattttgata gcgaagagat tctactagat
      601 tatgcatctc aggtggaatc agattggccg cttacacatg gtcaattttt ctctattttg
      661 ccgatttatg attcaggtgg atacttagag aaagtgtatc aaactgctaa atcggtagaa
      721 gcccaaacgt tccacgatgc gatttgtgcc cttatcgtag aagagctgtt tgaatatgca
      781 ggcaaatggc gtaatattcg tgtgcaagga ccgacaacat ttctaccatc cttgactgta
      841 caggtagcaa tggcaggtgc catgttgatt ggtctgcatc atcgcatctg ttatacgacg
      901 agcgcttcgg tcttaactga agcagttaag caatcagatc ttccttcagg ttatgaccat
      961 ctgtgccagt tcgtaatgtc tggtcaactt tccgactctg agaaacttct ggaatcgcta
     1021 gagaatttct ggaatgggat tcaggagtgg acagaacgac acggatatat agtggatgtg
     1081 tcaaaacgca taccattttg aacgatgacc tctaataatt gttaatcatg ttggttacgt
     1141 atttattaac ttctcctagt attagtaatt atcatggctg tcatggcgca ttaacggaat
     1201 aaagggtgtg cttaaatcgg gccattttgc gtaataagaa aaaggattaa ttatgagcga
     1261 attgaattaa taataaggta atagatttac attagaaaat gaaaggggat tttatgggtt
     1321 ctgttgcaaa gtaaaaaaat atagctaacc actaat
//
LOCUS       DAKTIO010000436          844 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3401A
            SAMN04868224-rid16188433.guided.370, whole genome shotgun sequence.
ACCESSION   DAKTIO010000436 DAKTIO010000000
VERSION     DAKTIO010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868224
            Sequence Read Archive: SRR5415243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516816.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 17:58:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,033
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868224-rid16188433.guided.370"
                     /isolate="3401A"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            318..803
                     /gene="lnu(A)"
                     /locus_tag="O0293_003033"
     CDS             318..803
                     /gene="lnu(A)"
                     /locus_tag="O0293_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011645015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(A)"
                     /protein_id="HCY0281816.1"
                     /translation="MKNNNVTEKDLFYILDLFEHMKVTYWLDGGWGVDVLTGKQQREH
                     RDIDIDFDAQHTQKVIQKLEDIGYKIEVDWMPSRMELKHEEYGYLDIHPINLNDDGSI
                     TQANPEGGNYVFQNDWFSETNYKGRKIPCISKEAQLLFHSGYDLTEKDHFDIKNLKSI
                     T"
BASE COUNT          314 a          116 c          134 g          280 t
ORIGIN      
        1 atatgtcgtt taataaagtt ttgaaatact tgatttcttt gttttttctc agtatacttt
       61 ttcatataat tcacataaaa caactttgtt tgaaatacga aaagacgttg ctttttctcc
      121 tttctatttt tcgtttagct tagacaccta aaacgataca atagtatgct ggaaaaggca
      181 actttttttg tgcttaaaac cagtcatatc aatggaataa gggtaactat cacagcttgt
      241 aatagttacc cttattatca agacaagaag aaactcgttt tcaacttgtt tcaaattccc
      301 aagttaggag ggataaaatg aaaaataata atgtaacaga aaaagattta ttttatattt
      361 tagatttatt tgaacacatg aaagtaactt attggttaga tggtggctgg ggggtagatg
      421 tattaactgg aaaacaacaa agagaacaca gagatataga tatagatttt gacgctcaac
      481 acactcaaaa agttatacaa aaattagaag atataggata caaaatagaa gttgattgga
      541 tgccttcacg tatggaactt aagcatgaag aatatgggta tttagatatt catcctataa
      601 atctaaatga tgatggatca attacccaag caaacccaga aggtggtaat tatgttttcc
      661 aaaatgactg gttttcagaa actaattaca aaggtcgaaa aataccatgt atttcaaaag
      721 aagctcaact tctttttcat tctggttatg atttaacaga aaaagaccat tttgatataa
      781 aaaatttaaa atcaataaca taattgcttt attccaattg ctttattgac gttgagcctc
      841 ggaa
//
